LOCUS       DAOOQP010000001        66411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_447_length_66411_cov_6.534963, whole genome shotgun sequence.
ACCESSION   DAOOQP010000001 DAOOQP010000000
VERSION     DAOOQP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 66411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_66411_cov_6.534963"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLK75_00005"
     CDS             <1..848
                     /locus_tag="PLK75_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY37659.1"
                     /translation="MLSELISKEKNNVSVNFDKPESVIEVITDETRLFQVMLNLLNNA
                     VNFTEKGRIDFGVKIMSEYLQFYVKDTGIGIAKELHQKIFERFYRHSGSLSKTHRGTG
                     LGLTICKSLIELMGGNIMVDSELGKGTCMSFTIPYLKPVTSVEPGTFRVVAANPDFAD
                     YKILVVEDDQASLEYLLAVLRKIGFRFINIAHNGNTAVEAAIRETYDVILMDIQLPDI
                     SGIEATKRILKHDPAARIIAQTAYAMQNDKDTCTREGCIDYIAKPIKINELSLVLSKH
                     LVKSL"
     gene            complement(869..3028)
                     /locus_tag="PLK75_00010"
     CDS             complement(869..3028)
                     /locus_tag="PLK75_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY37660.1"
                     /translation="MKKFAFLLVSLVILISGTLRADEGMWLLSLIGKNYDDMKAKGFQ
                     LTPDDIYNINNACIKDAIVGLSREGRPFWHFCSGEIISDQGLILTNHHCGYGMIQAHS
                     SIEHDYLKDGFWAYSKDQELPNEGYCAEILIRIEDVTSRVMKSVTSEMGEADRNAAIK
                     EASSEIIKEAEKGTYYSAQVSEMFHNNQYFLLVYEIYKDIRLVGAPPSSMGKFGGDTD
                     NWMWPRHTADFSMFRIYTGPDGKPAAYAKENIPLKPRYSLPISIAGVKEGDFAMILGF
                     PGTTDRYLTSYGLQETIDITNTYRYNIRTVKLDILRNEMKNNPEVRIQYASKYASSSN
                     YWKYSHEQNIALQKLNTIENKRAIENEFVAWTNADNNRKAKYGNVLNNIKNIYESRKA
                     DAYNRQFILEALLQGAEIPLFAYQISNQIGRLNENSTAADKEKIKTSIHNFYKNFDAN
                     TDKKLVAAMFSYYASNVDPMNYPTVFLTIIKKYKGNFDKFAADMFLKSVFSDEQKLLA
                     LVDAPNAKILDRDPAVIAGNSIYTKYQEINQSYRDKSSGLEREIRLFNEGLMEMNSDK
                     MFYPDANSTIRLTYGNVLGYRPADAINYKYYTTLEGVMQKEDPNNDEFIVPEKLKELY
                     KNNDFGRYTNAEGKLVTGFITNNDITGGNSGSPVLNGKGELIGTAFDGNSEAMSGDID
                     FEENLQRCINLDVRYTLFIIDKYANAQNLINEMNIVE"
     gene            3125..4006
                     /locus_tag="PLK75_00015"
     CDS             3125..4006
                     /locus_tag="PLK75_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37661.1"
                     /translation="MKVVFILFFCFVFSIMGFSQPEPVLTQNDLMQTKIERSALQYMK
                     INLLRTYIMEVEKNELTGRKALVSEVYYDKNACPESIVYFDSAGNRSQYTHYLYNSDL
                     YKTEEIFFNSDSTLIGGVAIECDSSGITKAFIVYNKNAEITSEYKFLVDPVKNIIVAQ
                     ISNKKNNKQNYKYYHITGGMKDGRIASVENFNENGILNDSISYHYNQAGQLSFKQYFD
                     SDRKAMQETWFFYNDEGAVEKTQEINILTSQQRSFSYAYDEYGNRTSLIESDKNNKII
                     SYYRFEYFSRVLPPEKR"
     gene            complement(4017..4910)
                     /locus_tag="PLK75_00020"
     CDS             complement(4017..4910)
                     /locus_tag="PLK75_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOY37662.1"
                     /translation="MKKWFNYFIYISIIFLIVFLVRADYFVVPVVRNVFHLVLAFVFV
                     FIGFFLDAYSWYKALHLFNCKQVKYLDCVASEGLSIFGKYIPGKIWVILGRSAYISKR
                     YDLNEKEVASISLNVQFVSLWSALLLGILGLAFIQVSWYIVLGSIALLIIFSLILFTP
                     LFHRLFTVIMMKLLRKRFDIPQLTISSVLFVLPFSVLCWLTWCLGFWFLAISLVPEHI
                     PLFAGLFYALGASLGMIAIILPGGLGMREGLLSLTLIAAGLASDFSVTVSVSSRLWYL
                     IGEGFIFFTGLIISRFYKLRK"
     gene            complement(4926..6023)
                     /locus_tag="PLK75_00025"
     CDS             complement(4926..6023)
                     /locus_tag="PLK75_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY37663.1"
                     /translation="MLFNKTIAVVVPAYNEETQIVQVIESMPDFVDRIVVVNDLSTDN
                     TADVVKTYIDTDSGNYYNPRIAEKTVTPNRYNEADVVLQQIFKDEIRYFTPSAIQNTQ
                     PENDRIILINHLKNGGVGAAIARGYKWCKDNEIDCTAVMAGDGQMDPSELEMICKPVV
                     AENLDYVKGNRLMHKSAKFVIPKQRHFGNSVLSILTKLSSGYWRVSDTQTGYTAISLD
                     ALKSIKLYNIYPKYGMPNDMLVKLNIASSSIREIEIKPVYRVGEVSKMKVNKVIPRII
                     WLLFRSFFTRLVRKYFLRDFHPLFLFYMLAFLLSIANIPFLINIVYNMFTTDVTPISY
                     LIIFIFLFISSLQSFFFAMWMDIQDNERLYK"
     gene            6247..7479
                     /locus_tag="PLK75_00030"
     CDS             6247..7479
                     /locus_tag="PLK75_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOY37664.1"
                     /translation="MDLLQIKQRFGIIGNAHALNRAIEVAVQVASTDLSVLITGESGV
                     GKEILPQVIHYESKRKHNSYIAVNCGAIPEGTIDSELFGHIKGSFTTAINDRKGYFEE
                     ADKGSIFLDEVAELPLSTQVRLLRVLESGEFIKVGSSKVQKTDVRVIAATNVNIMNAV
                     QEGRFREDLYYRLNTVPLHLPPLRERGEDIALLFRKFAADFAEKYRMPPIRIDDDAKD
                     LLLKYPWPGNIRQLKNITEQISVIEQNRLIDAVILSRYLPAIYDNRLPALLSKVDEKK
                     FNSEREILYNILFDMKKDMNDLKKLVNEIIAQTPHLDLQEHVHDITGEFVQDSDAEIR
                     IEPRVVVANTIQNLQHKNFEDTSEIVEENLSINDKEVELIQKALEKNSGKRKLAAKEL
                     GISERTLYRKIKEYNIDA"
     gene            7479..7997
                     /locus_tag="PLK75_00035"
     CDS             7479..7997
                     /locus_tag="PLK75_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOY37665.1"
                     /translation="MKQTQPIFQIIMVAAIVAVFASCKGSYSFTGASISPDVKTIQIS
                     YFPNRASNVNPSLSSTLTEALKDRFLSQTSLTIVNSQGDLTIEGEIADYRVTPQAYQG
                     NETAALNRLTINVRVKFTNTKDPSKSYDTQFSRYADFESSKNLASVESQLITEIVDQL
                     TEDIFNKAVVNW"
     gene            8001..8756
                     /locus_tag="PLK75_00040"
     CDS             8001..8756
                     /locus_tag="PLK75_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37666.1"
                     /translation="MNKEAYRSYLQKPALMDFNSLSDMDALLKEFPYFQTAWLLYAKN
                     LQLIDDVRLGSQVKAAALHTPDRAMLYRLLHEVHEKKSEKYGCQFAKTSSIEKNIIID
                     SANKTDISQIIISDGLLDFGFMRIEFAPESKFSASLPAEQSTVFESNTTEPDVKDSRI
                     KNKLLIDRFISDKPKIIPKETEIALNTDLPDIERGENEELISETLAQIYVKQGYFEKA
                     LRTYEKLSLKYPEKSIYFATQIKKIEELLDKTL"
     gene            8759..8980
                     /gene="secG"
                     /locus_tag="PLK75_00045"
                     /pseudo
     CDS             8759..8980
                     /gene="secG"
                     /locus_tag="PLK75_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
     gene            9126..10898
                     /locus_tag="PLK75_00050"
     CDS             9126..10898
                     /locus_tag="PLK75_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37667.1"
                     /translation="MVLNNIKSTLNNRKTEFIIFTFFVLLNSSFLLCCNNIHSLDGPQ
                     HLYNSNVICELLQGNNTVSEYFSLNRVPVGYLTGPVILSALNALFPANIALKILVFIC
                     IAGLAYSFRYMVYSINKVTTYTTLLIIPFTLQTFLGMGYFTFSLSLAFYFICIGYVIR
                     NFDSLRFKNLSILGFLLLMTYFTHMVVFSFTMGTIALFVVFRFLREMIQRNQSVIKRY
                     LIIAMKIAIASLPAVVLAVIYAYHVFKLNTPIPNPVITTTELYRNVFIMKNLIAFDLT
                     AEAIMGKTIFFVLIAAFSYVLIVLLHSVITKGKQLFRSGNGKSDRLFFFCVAVLFFIM
                     YVFMPNVVGTGAVSMRVLVLFYLVFVLFLALQKYSPVFLVLMLGVIFYSSVQRSYIAS
                     DSLKSLSRDVDEIMEMNDYMEPNSTYFAMNYSTYWQHSHIDCYIGVDKPLINLANPQC
                     LGHFPVVWENKLPCLYLGNHASHELGVFWNSAGIDRTIHIIDYFVVWGYGSFINEKSS
                     LKTTIDNYYELVAVSSRGNAALYRFKLGSLIDESLEKMKKSPEWLAQLQRKAQEKQIP
                     LHNAMLLDIFYLIDYYNLQAKDTN"
     gene            10941..11918
                     /locus_tag="PLK75_00055"
     CDS             10941..11918
                     /locus_tag="PLK75_00055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOY37668.1"
                     /translation="MIIRFSSIGDIVLTTPVVRCIKQQIEGAEVHYLTKPQYKFLLEE
                     NPYIDKIHVLGRFSETLSQLYDEHFDYIIDLHNNLRSLRFKHRLKVIDFSFHKLNREK
                     LLLTWFKLDKLPDIHIVDRYFATVSLFDVENDGKGLDYFLPENDPKKVHPLLEDLPLV
                     YFVVAIGGQHETKKLPPYKLAELCRLLQYRTILIGGNQDSEAASEIVEMTEGADVTNL
                     CGLISVNDSAALIRHAAVVITHDTGMMHIASAFRKIVFSIWGNTIPKFGMYPYMPDER
                     SKIFQVEGLPCRPCSKLGRKRCPKKHFRCMTEQNVKTVALEANKIAFQC"
     gene            12131..12201
                     /locus_tag="PLK75_00060"
     tRNA            12131..12201
                     /locus_tag="PLK75_00060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12163..12165,aa:Cys,seq:gca)
     gene            complement(12236..13411)
                     /locus_tag="PLK75_00065"
     CDS             complement(12236..13411)
                     /locus_tag="PLK75_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOY37669.1"
                     /translation="MPANKSEIWSKSSCYSFEVLDNYVAGKLPKEASHAIESHLVDCD
                     VCSDVVEGLQAMPHNHDVKKEILAINREIDKKILHKRNRNVLGISTYSLRFAAAAVLV
                     TFAVCVVIFMNYDSKQALYKPSDLSKNSLSKNKEQSDDKLRSETSANEELAQNIPGQK
                     IDAPIKEAEPYPASASESRNYAEPEEKTVFDELFNIDEDDHDASGSGDASEEHSIADS
                     NNKQVNGNAGGFAYSDNTTATGKTQEKQHQRQESSALDKKDSDRESAPPSATHDLVVV
                     ESDALIMEEPISDYFSVEVKPEYPGGEAAMLKFISDNIAYPIVAKENRIQGTVYVSFV
                     IDENGWVTTPKVIRSVDPLLDQEAVRVISMLPQWSPGKQFGVPVKVSYVIPLIFKLD"
     gene            complement(13398..13982)
                     /locus_tag="PLK75_00070"
     CDS             complement(13398..13982)
                     /locus_tag="PLK75_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY37670.1"
                     /translation="MFGIKLNKNQPDEVLIALYQQNNKPEIIGELFKRYTSLIYSISY
                     KYLKDSDKCKDMAMDVFELLIEKLKTHQVVNFKSWLHTVVRNECLMMLREETRSYSFD
                     DTLKNGSNNFMEEDPFLHLDYVEREKKLVCLEICIEKLNIEQKTCVELFYLKQMSYVD
                     ISDETGYDLKKVKSYIQNGKRNLKILIENYNAGK"
     gene            14102..14530
                     /locus_tag="PLK75_00075"
     CDS             14102..14530
                     /locus_tag="PLK75_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOY37671.1"
                     /translation="MQAPEERIHKFIGKHHVFTLATTVAGQPWCTSCFYAYWQNRVSL
                     VFTSEQHTRHVNEFTKNKQVAGTIHLETKIVGMIRGIQFIGEVVRPDEAETARLIYLK
                     RFPFAVFMDSEFWVLRIDSIKMTDNRLGFGKKIHWTRDEN"
     gene            14520..15389
                     /locus_tag="PLK75_00080"
     CDS             14520..15389
                     /locus_tag="PLK75_00080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY37672.1"
                     /translation="MKININQIFNDYELIDCGDGEKLERFGNKIIIRPEVSATGKPFL
                     TKAEWQSLADAIYLDNIGTGGKWSFFNTPFDSWEVMYSVCGKPLKIRLQLSSFKHVGV
                     FPEQAINWNFIAGLEGLFGRKLQILNLFGYTGVASLVAAGNGHRVTHVDALKQVVQRG
                     KTMMVENNLDGIRWITDDVLRFVAKEQKRGNSYEGVILDPPAIGKGPGGEIWKLEKHL
                     EPLLAEIRKILGNRSFVIINLYTQIINQQDANKLVSDYFPDFVTETSDRLLGESRFGS
                     SVDYGIVVRLFRK"
     gene            complement(15403..16527)
                     /locus_tag="PLK75_00085"
     CDS             complement(15403..16527)
                     /locus_tag="PLK75_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOY37673.1"
                     /translation="MKFQEIIGQEEVKKYLIQTVTENRVSHAQLFYGPPSSGKLALAV
                     AYAQYICCANKSATDSCGECASCRKYAKLIHPDLHFVFPVISAQGKSSISDTYIESWR
                     LNFLSNPYITLNEWLECIGAENKQGSIYAEEAREILHKLNLKTFEAEYKVMIIWLPER
                     MNNSTANKLLKMLEEPPDKTLFVLVADNHEEILPTILSRTLPVKIPRIDNKSLSEALV
                     SKFGINTSEAREICNMSEGNYLEAVNHINTSEELVFYFEKFVALMRQAYSRNIYDLVK
                     WVGEIAGTGREKQKSLMEYSLRMIRGNFLINNSLPEMSFLVKRERDFSERFSAYITPN
                     NVEKIAEAFEKAAYHIERNGNARIILTDLCFTMMALLKAR"
     gene            16716..17903
                     /locus_tag="PLK75_00090"
                     /pseudo
     CDS             16716..17903
                     /locus_tag="PLK75_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
     gene            17895..19100
                     /locus_tag="PLK75_00095"
     CDS             17895..19100
                     /locus_tag="PLK75_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOY37674.1"
                     /translation="MKESFSVDDYNFNRKRVLMRVDFNVPLDKEFNITDDTRIVTAIP
                     TIRKILADNGSVILMTHLGRPKGGFEDKYSIRHIVPYLCKKLELDVKFPGESIGPEVK
                     KMADELKPREVMILENLRFHPEEEKNDENFAKQLAELGDVYIMNAFGTAHRAHASTYM
                     VAKFFPEDKMLGYLVESEINHIERALHKGEKPFTAIVGGSKVSTKIDIIKALMDKVDN
                     LIIGGGIAFTFIKAMGGSIGNSLVEDDYIGVAKEILDKAILTNVKLYLPTDCLIADKF
                     DNEAHIRHVKINEIPDGWSGLDIGVKSAETFSKVIERSRTILWNGPMGVFEMDHFSNG
                     TIKIALASSRSTDHGAYSLVGGGDSIAALNKYGLAHKISYVSTAGGALLEYIEGKELP
                     SLKAIRTKI"
     gene            complement(19179..19637)
                     /locus_tag="PLK75_00100"
     CDS             complement(19179..19637)
                     /locus_tag="PLK75_00100"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOY37675.1"
                     /translation="MGKKSKSTGLIMIIIAVLFFTADIFSIFQVADYYKHSQQATGVV
                     TNIFQTSTSGPHGHNPSFSYSIEFTASNGNQYVAVTESRNFELGDEVMVTYDSRNPLI
                     SFASFWDIWIFVIICSLGSIIFLLIGYSKLKSAKPIESTISYQAKLTKTP"
     gene            complement(19802..21994)
                     /locus_tag="PLK75_00105"
     CDS             complement(19802..21994)
                     /locus_tag="PLK75_00105"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY37676.1"
                     /translation="MKKLMFFFFILLLSGSLFAYPDEGMWIPGYMSKMNYADMQKLGC
                     ELTPEQIYSINNSSVKDAIFQLLGEGGYGFCTGEIVSSKGLLFTNHHCGYEAIATLSS
                     LENNYLDNGYWARNFDEEISVPGVSVSRVVRIEHVTDKVFAGITEDTPAEDREKMIAK
                     AIDKIETEAIEGNHYNANVKEMYKGGEYYLFVYEVFGDVRFVGAPPSSIGKFGGDTDN
                     WMWPRHTGDFSIFRVYMSPDGKPTEGYDKNNVPYAPIHYLPISIKGVKEGDFTMIMGY
                     PGETERYLSAAGMEYKRDYYDPILVTLLGTMLDIMKVDMNNNPEIKLAMADVYANYAN
                     GRKLFDGEMQTLKTTDAIAQRRSFEEGFMQWVNADEKRKGKYGEAFENLTANYKTYGE
                     ATKAMYYLAFGLLQGSSRAMDVQSYMALASYLEDPKNNQEMIDLTVDQLKIEMEENFK
                     KYFPATDRKVFEAMLKLYIKDIKPDERNSVFADFIFKNYKATSEDASIEKFVADVYTR
                     SIFTDKTRMEAFLNKPNLKLLQKDPMYNFVETIFNGVMLVQMSYIGIMDEVDSAEKLY
                     IEAIRAYQTDRDFYPDANSTLRLTYGSVQDYDPRDGVKYHWLTYADGIIEKMDNSDPE
                     FVVDPKLEQLIRAKDYGRYADETGNLPVCFLSDNDITGGNSGSPIINGKGELIGLAFD
                     GNWEWLCSNLIFSTSLQRTINVDSRYVLWVIDKFAGAQNIINELDIRQ"
     gene            complement(22141..23133)
                     /locus_tag="PLK75_00110"
     CDS             complement(22141..23133)
                     /locus_tag="PLK75_00110"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37677.1"
                     /translation="MKKLSLLFVIGFFAAFNAMSQCTPDEGCEDVLDPGQICPMVFDT
                     GYVDVPYTQVITFIPPETYNYNGYDLTVQKIVINSVTGLPDGLDWDTNAEQFIPTNPI
                     TRYCGIIFGTPTTEGEFPFQFSATVTVLLYGSVPVDVPVTQETLGYDAKVVIRPQNQA
                     PPVAEFTANPTTSEGGVPIAFTDASFNATSWEWTFEGANEPGSTEQNPTATWNSMGVY
                     DVTLTVTNGNCTPPKTSTFIAENYITITTNVFVNPNFTKICTVYPNPANGIIHVEGLG
                     ISAVEVYNTIGQIVFRSEPNADKAVLDLSHLQKGNYMIVVKTSKGNAHTSLTLN"
     gene            complement(23219..25687)
                     /locus_tag="PLK75_00115"
     CDS             complement(23219..25687)
                     /locus_tag="PLK75_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY37678.1"
                     /translation="MIKSLLSTTLFILFSTTLLLSQTAKITGTIKDNSNNEFLYGVNV
                     IVTGANDTVNVKTGTTTDFDGKYTLDLSAGTYTITYQYIGFHRERMKITLKAGEVKTL
                     DLSLKPSAELIDEVVVSAGKFEQKLSDVTVTMEVIKPTMIQNTNTISLETSVNQITGV
                     DVSDQQLSIRSSSGWSYGAGTRVIMLMDDLPIVSADVGDIKWNYLPVENISQIEIVKG
                     ASSALFGSSALSGVINVRSAYPTDKPETRVSYFIGVYDSPKTESYKWWDEDFYFNDSL
                     FYQHIPLRNGLYYWIKNPMYSGLNLFHSQQFGNLDITMGGSHFVDEGYRQKEYEIRSR
                     ANVNWRYRSKKIEGLAFGMNTNFMAVDKSDFFFWHSDTLPLNTPEVDKLFAPTQAYRV
                     NIDPFIYYYKPNGSKYKIRTRYFGTRQYIPNDTAKNSNGDLYYIEYTYSTNLGEKGNV
                     TAGGVNGYSEVHSNLFGDHFHNNFALYGQLDWKFGRLKASVGLRGEYYKVDTAQTESL
                     VRFKNFEIPFKPVFRAGLNYQLFEYTFLRASWGQGYRFPTISEKYTASDLGSMVKILP
                     NPYLQPENGWSAEIGVKQGIKLWEGWKGFVDLSFFMQEIYNMIEFTIGKYDPVTLLPV
                     SLYESGDFGFQARNMADARILGSELSIVTSGKLGPLKTTVMLGYAYNYPVNIAGADST
                     ASTDNPILKYRYVHSFKGDFDFEAKRLTFGFNWILNSPMKNVDRLFCDERDPSTLTTD
                     QYNLYQFYYFMSNAALLPGYWDYRIDHANQPYVSFDARLGFKFSEIIRISLLVKNLFN
                     SEYVGRPGDLRPMRRYELMLTAKF"
     gene            complement(25847..30145)
                     /locus_tag="PLK75_00120"
     CDS             complement(25847..30145)
                     /locus_tag="PLK75_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY37679.1"
                     /translation="MSKKIQIALVIFLIFAGISGVKAQTNSNILIHNIHYYSYDQDMW
                     GAGGAFNLDFDYELFHLEFSENPTLDFIEDVPLLGPVGIAWEAGFWAYLSSTFSIHGF
                     TLGSIDVNYPVEITLDFPDHYSFDHGETVTVFTSYEVEDGWYLDTHFPTVGIIALDLE
                     YGFGVYMNIIVCMFDCETIPILPTLSIPIDPDYTDPLPHDSIAIFYLNGYTGEVVYPC
                     IDEITGLPMLCEDDILPIVIPDWFGIGLTGEIDLPYVETEDYLDPDTQCLTAYGQDEW
                     IWFNLNILTFLSWIAGFIPPPEGPAIQEIIGIINGGTITYEIMPGVNAIIDYYLLHLD
                     LHMSSYMTQEFSFCPTIWVTLGFPTPLVYSVVDPDNGNTVVDQGTAQDITFEVNNDLN
                     ITYPCYDWDSMPVTVAYHFDNDFNNHTWDSIAFDFIVSVFYAHVTIPFDFPIKSAEMP
                     EFELPPIVQEYKDVGYSQTLRYASPEINIISPPQIDLTSQKSSNVIPEPEIKSDSMPL
                     KDIEFEIGPLFEQSFPLGYIPLTWYDQTWEMEGFLLDTVCPGTWLYPLPELDMDINAQ
                     DVLCFGDTSGVITVTAIFSSGPFTFTYSWGSEHTHMSPIDSLIVPSGYYFVTVTDVYG
                     CSVSGEINIADAFPPIVTYLHADDVLCAGEPTGNLYSYVTGGAPPYTYLWEPSGSTDP
                     NPVGVYAGWHYLTVTDSVGCTHLDSVFIDEPDEPLEMTWEYGNVTCYGYTNGFIDLTV
                     TGGTPPYYYYWSNNQMTEDLYNLADGAYTITLVDSHGCDIEHTFVIIMPPPLEVELII
                     QDVLCYGENTGWVNAVVTGGTPPYTYLWSNGATVQDLFNQYAGIYSITVTDANDCEAY
                     AIGRIDQPDLPLTAYIDPTDVRCFGEENGIADLTVFGGTPPYYYAWSNGEISEDILDL
                     EPGMYSVTVFDENDCVAYAQVQIYQSEAPMAGTITGTNVRCNGGSDGNVYINVTGGTP
                     PYHYAWSNGSWEEDQFNVEAGIYRVTVTDDRYCHYIMSYEVTEPEPYVIETYPDPQIC
                     YGMIATLGLNLVTGNTPPYVVLWSNGATGMTTNVHPLSDSTFTVMVVDSNNCVSNTLP
                     ITVHVYDPIAMTVDAESEHVCPGEFVDFDVNISGGGVSGNYVMINDSMYYLPISLQVF
                     NDTTFEFVIFDSCNYQFVRIEKEITTYPLPPLSISADKYNGCAPLTVSFRENSEDIGQ
                     RYIWNFDDGDFENLSFDKYPVHTFFNATTYHVRLEITSAEGCKRDSTIGIVVFPVPEA
                     EFVSSSTSVHMASPAVSFTNFTEGGFFFNWDFGDGTSTTVTNPQHEFSFPGVYHVTLS
                     TESLYGCVDSTGVDITVTNETMIYAPTAFTPNHDLINETFRIIGDGIDRQTFQMEIYN
                     RWGERIFVTNDYEVGWDGMSHDKECPEGIYTWIISFEDPFGNIYTQSGQLSLLR"
     gene            complement(30147..30686)
                     /locus_tag="PLK75_00125"
     CDS             complement(30147..30686)
                     /locus_tag="PLK75_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37680.1"
                     /translation="MLRVIHTIVGFFVVVVSFAQVPLNLTTNGELQGTGDAYITTPGE
                     SVLIPNTHVKIIPPEHFMYSAELQGFVHGATSSTMQISEIIGTSYIMVSKSLTPEYVE
                     SQNFRYIEKRDITTNDGHDGCVFILGFTNKGVDYERMMFLAGDYNNTIWINVSYPAVS
                     RNLLFDIFNNSLLTAQFIK"
     gene            30864..32039
                     /locus_tag="PLK75_00130"
     CDS             30864..32039
                     /locus_tag="PLK75_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37681.1"
                     /translation="MKRTLLLLPVLFAVVISCGSGKNNKSAAGKNDNPVKSGDTIVID
                     YPKDTVFDQIASLLSGENASSFNDTFAQSQYWKDYKISIDTPWQRIENERLSIIYNWS
                     DSVIKPLINDTSLLFYPFSGPDFVHSYYLFPNANEYVFIAMEKLGTIPALHKSSFEQL
                     KAYLDATNFAIRDIYKRSYFITGNMEQDLRKQQVDGVLPVLLVFLHKTGHEIYDFGYY
                     RLNNDAKTFTKIDKPTNSLDQVECIRFLILKKGDNKLKTFTYFYADISDSGFAKTKTL
                     KKYLDNMRSCNTFIKSASYLSHYGTFSNIRDLTLTKSSAVLEDDTGVPFRYFTSDKWN
                     YKLFGVYVKPIADFPSSYLYQKDLAAAYDSVGAQALPFSLGYHWRTGEQNWLLFYRK"
     gene            complement(32045..33169)
                     /gene="hemW"
                     /locus_tag="PLK75_00135"
     CDS             complement(32045..33169)
                     /gene="hemW"
                     /locus_tag="PLK75_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOY37682.1"
                     /translation="MPGIYIHIPFCRRKCVYCDFYSVAQTGKTDDLLNGILLEAARNK
                     RFFSSTPVSTLYIGGGSPGMLTAEQISSFVNQLGEIYDLRWLQEFTVELNPEDAIPEY
                     LETLRSSGVNRISIGIQSTHNRHLLFLGRRHNSEIALQSLKNTRNAGFSNISADIIFG
                     IPDLSQNELAESIAILTEAGLTHISAYLLTAEGNTLLVRRLREGKTRLPDEEICNRQY
                     SLLCDTLRTYGYDHYEISNFCKPGYRSQHNSSYWDNSIYLGLGPSAHSYDGNKRYYNP
                     PDLNKYLYSIPKNQSVTVCENLENSDKINEHIMTRLRTSDGLNLHEFENLFGKSEVAG
                     LLENSKKFIQIGSVKNNRGIIIIPEPYWLISDSIISELFV"
     gene            33290..34054
                     /locus_tag="PLK75_00140"
     CDS             33290..34054
                     /locus_tag="PLK75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOY37683.1"
                     /translation="MKIITFNLNGIRSAQAKGLMNWLKIENPDVLCVQETKAQPEQID
                     FHEFQEMGYSVYFHSAQKPGYSGVALFSRVAFDNVTYGIGVKEFDSEGRFIRADFNNT
                     VLINSYFPSGTMGDERQEVKMKYLDAFYNYTEMLKKVQPNLIVCGDFNICHKEIDISK
                     PENKKGVSGFLPEERAWVSKFIDLGFVDVFREFDKSPGKYTWWSYRAGARKKNLGWRI
                     DYHMVTVPLKSKLSASSILSDIAMSDHCPVVMEIAL"
     gene            34110..34694
                     /locus_tag="PLK75_00145"
     CDS             34110..34694
                     /locus_tag="PLK75_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOY37684.1"
                     /translation="MSSTNYFAHETAIIDEGCQIGHGTKIWHFSHIMSNCKIGENCNI
                     GQNVVVSPEVVLGNNVKVQNNVSIYTGVVCEDDVFLGPSMVFTNVTNPRSAVNRRGQY
                     AKTLVKTGASIGANATIVCGHDIGRFAFIGAGAVVTKHVPDYALVIGNPARHAGWMSE
                     YGHKLQFDENGIAICPESKEKYVLKEGIVEKISM"
     gene            34694..35659
                     /locus_tag="PLK75_00150"
     CDS             34694..35659
                     /locus_tag="PLK75_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY37685.1"
                     /translation="MEPSVKNFALVGVAGYIAPRHVKAIKETGNKLVACLDKFDCVGY
                     LDSFFPEADFFYEFERFERHIDKLKYTDRKIDYVSICSPNYLHDSHIRFALWNRAHAI
                     CEKPLVLNPWNIEALMKLEQETNCNIYNILQLRLHPAIIALKSEIENGPKDKIYDIDL
                     AYITSRGKWYDYSWKGNSEKSGGVATNIGVHFFDMLLWIFGGIEKSRVHISETHKAAG
                     FLQLKNARVRWFLSIDYNDIPEAIRSKGQRTYRSITVGNREIEFSEGFTDLHTLSYRN
                     ILEGNGFRVAEAMPCIDAAYQIRNAKPIGLVDEYHPLCAVKNHIF"
     gene            35724..37013
                     /locus_tag="PLK75_00155"
     CDS             35724..37013
                     /locus_tag="PLK75_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOY37686.1"
                     /translation="MYQSIINKQKKISLIGLGYVGLPIALEFAKHVPVVGFDIKPERI
                     DLMKKGIDPSNELPKEAFAGCDIMFTANPEDLRDCHFHIIAVPTPIDDHNLPDLTPVI
                     KASETVGRILKKGDYVVYESTVYPGCTEEDCVPVLEKMSGLKFVEDFKVGYSPERINP
                     GDKEHTLTSIKKVVSGCDTESAENIAKVYEIVIKAGVHRASSIKVAESAKIIENTQRD
                     VNIALMNELSMIFNIMGINTYEVLEAAGTKWNFLKFVPGLVGGHCIGVDPYYLLHKAK
                     ELGYHARMINSGRAINDGMGAYVAEQTVKKIIAMGKEITNSRVLVMGVTFKEDVTDIR
                     NSRVVDILNELSKYNVKYEVVDCHANHHEVEEEYNLKMVEKPEGKYDAVIVAVNHKEY
                     KDLDENYFSSILKDRGLIMDIKGMYRGKIKNIAHWTL"
     gene            37143..38186
                     /gene="galE"
                     /locus_tag="PLK75_00160"
     CDS             37143..38186
                     /gene="galE"
                     /locus_tag="PLK75_00160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOY37687.1"
                     /translation="MKRPVVLVTGGLGYIGSHTVVELQQSGFDVIIFDNLSNSFIEVL
                     DGIEKITSVRPGFFKGDVTNKKDLEALFDTYDAIESVIHFAALKAVGESVENPLKYYH
                     NNVYGMMNLLQASIKHRVKNFVFSSSCTVYGQPRILPVDETAPIQPESPYGNTKWICE
                     EMLRQVISSGVAMKAISLRYFNPIGAHPSAEIGELPQGVPNNLMPYLTQTALGIRKEL
                     VVFGNNYNTADGTCIRDYIDINDLSNAHVVALAFLSNGSAKPYDVINLGTGLGVSVLE
                     MIRAFEESCHVSIKFRIGDRRGGDVEKVWANADKALQVLGWKTRISLHETMKNAWYWE
                     RRFRNGETAIKPL"
     gene            38265..39320
                     /gene="rfbB"
                     /locus_tag="PLK75_00165"
     CDS             38265..39320
                     /gene="rfbB"
                     /locus_tag="PLK75_00165"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOY37688.1"
                     /translation="MKKTIIITGGAGFIGSHVVRLFVTKYPDYRIVNLDKLTYAGNLA
                     NLKDIENLPNYRFERADIVDGDDMIKIFEKYQPSGVIHLAAESHVDRSISNPTEFIFT
                     NIVGTVNLLNAARHIWQGNFEGRRFYHISTDEVYGSLGDEGFFTEETAYDPRSPYSAS
                     KASSDHLVRAYHHTFGLPVVVSNCSNNYGPNHFPEKLIPLAINNIKNMKPIPVYGKGE
                     NVRDWLYVLDHARAIDTIFHQGKNGETYNIGGNNEWKNIDLIRLLCQIMDKKLNRTPG
                     TGESLITFVKDRAGHDLRYAIDSTKLQRELGWKPSLQFEEGLERTVDWYLANEEWLSN
                     IVSGDYMQYYVQQYVKR"
     gene            39325..39663
                     /locus_tag="PLK75_00170"
     CDS             39325..39663
                     /locus_tag="PLK75_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HOY37689.1"
                     /translation="MTEEIFSTLPFAVTACNTEGIIVYMNEKSKATFEKDSNVSLLGK
                     SLFDCHSEASSAKIKELLQTGGTNAYTIEKNGQKKLIYQCPWFKEGMVAGLVEISLPI
                     PLEMPHFVRG"
     gene            39856..40206
                     /locus_tag="PLK75_00175"
     CDS             39856..40206
                     /locus_tag="PLK75_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37690.1"
                     /translation="MIWYGVYGPPDYTHYLEKFIGIRIPNTSYVWIKSYTPKGNISVY
                     PNPATDILYINMPEDMGETEIEIVDFTGKTIFKTRVWSSDYNINTSLFKSGFYVIICK
                     KDGILYKTPFLVSR"
     gene            complement(40260..40835)
                     /locus_tag="PLK75_00180"
     CDS             complement(40260..40835)
                     /locus_tag="PLK75_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY37691.1"
                     /translation="MCPKSKIQFEEIRRERRGLIMDTALRLFAEKGYHQTSISQIARE
                     AGMSKGLMYNYFDSKEALLGEIFQEGFNQAYENFDQNQDGILQKEEFVDFVYRIKSLI
                     DGNHNFWQLLVNLILQPGTLTSLGDFKLKGADDIDTLLREYFIALGYEDPEFEKTYCI
                     SVLQGAFIQYIFDHEKYPIDKVIERIVKLYA"
     gene            40936..43755
                     /locus_tag="PLK75_00185"
     CDS             40936..43755
                     /locus_tag="PLK75_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOY37692.1"
                     /translation="MKKSSYFLLLSIFILSFFSDVCAQAPVVVPAEHPGMVGLWTFDN
                     QSSLMQATTGSDLETHGTVNAISGPVAGDNAINIGVGSYLTCYHNIAGNGGGSEVNEY
                     TMVYDFKIPATGVWYCFYQANESNSNDGELFINPSGSIGRSTDGPGYSVYQVSPGEWY
                     RMVVSVDLGNYYKVYLDGALILAGGSLGVDGSYSLYPADDENNVLFFADNDGEDNSFD
                     IALTAIYNYSVNQAEVNTLGGYGHVIQPVLTGILPYLQTPTPNSMYISWHSDQTASTT
                     VQYGISEALGLSQTGSYEDISGNTWHTVKLTGLIPETQYFYKCISGSEESELYTFKTP
                     ALGVPQNGHFRFIIVGDSRTDVAKTTEIAYAAKNKAIELFGDDIYNQINLVCHVGDIV
                     SSGTEISQYKNEYFGPYACLSNVIPFMVSIGNHENEASNYYDYMKYEDFSDYTSFLLE
                     EKFYSFYLSDIQFIMLNGNGLVVNSIQTGWLEDKLSQSDASPTTDMVFAFVHQPGHSE
                     LWPDGNTAYVQEDVLDALKQNKKVQLLSYGHSHCYERGVIESQALDAIGDFYIMLSGG
                     AGSGLDRWGMYPNQQDYPEIQLALDHYCFSIVDVDLVNKSWDLYSYSLGNLDKPLDVV
                     LVDQYHRKLLQDAPLAPIALSPQINSNLLPLLVASEMQGADSLMTSQFQLTSNPGNYG
                     SPLLDVKRDWINVYWDSGAPDYLPIDLNQGIDLRRLQISAPLTAGNTYGWRVRYRDHN
                     LRWSPWSAEKTFTAHSDLQAYTDFSADVTEGISPLTVHFSDLSLPVVSAWAWDFENDG
                     TVDSNEQDPAHIYTQEGVYTVSLTTANGTETKDYYINAETNSLEEIINKGSDNVQVRP
                     NPFYISTAIDFTLKTAGKVVISVLDAKGNTIRVLENTVFNAGKHTVMWNAGDGSANRL
                     SPGKYFVKVTGAGIDAVKSVILIDK"
     gene            43757..44569
                     /locus_tag="PLK75_00190"
     CDS             43757..44569
                     /locus_tag="PLK75_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOY37693.1"
                     /translation="MKQLAVALALCMLAFYVTAGHPDSLPDGKVLSYYENGKIRSVTH
                     YKNGQLHGKFESYYANGTIKEKGLYKKNIQEGKWIKWYENGNLKSVAEFTSGKINGEY
                     RFYYESGVLQFIEKYDMNKKNGDWFYYYDTGVLKETQKYQNHKKIGTWYYYYPDGKLQ
                     GEILYDLSKKTGVWKEWDENGVLRLETPYNSNMMQGVKKKYNEKGVLIEETTYDKNLK
                     SGLWVTYYSNGNPEVKGYYKNGEYKDGEWQYFDENGNLKKTETYKAPSIIKK"
     gene            complement(44576..45493)
                     /locus_tag="PLK75_00195"
     CDS             complement(44576..45493)
                     /locus_tag="PLK75_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY37694.1"
                     /translation="MKQQKRVLITGCGGMLGESMYKVFCEKYQEVKATDIDLNESWLS
                     YLDVREFSQCVKIFDEYKPEIVLHLAALTDLEYCETNHENSWCTNALGTENIAFLAEK
                     YGCTMVYISTAGIFGGEQEYFTDFDTPNPLSHYAKGKYYGERFVLERVSKCFVFRPGW
                     MMGGGPRKDKKFINKIYKQIVAGKTELHVVDDKLGTPTYTLDFANAISKVLETGYYGL
                     YNQVCDGSCSRYDVAVELINILGLSDKIKITKVESDYFEKEYFAPRPYSEKLVNLKLN
                     KRGINYMRDWKICLQEYALQYIEDFKKRQ"
     gene            complement(45504..46592)
                     /locus_tag="PLK75_00200"
     CDS             complement(45504..46592)
                     /locus_tag="PLK75_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY37695.1"
                     /translation="MTRIKKILVICPAPEKVYPSQRLKYEQYFPHWQEAGYSITIAPF
                     MTHRLWAVLYKNGYFFTKFILTLWGYMRRIADVFRAPFYDLVYIHLWVTPFGGSMFEW
                     LVKLANPHIVYDIDDAVFKGHKSKMNWFIAFLKGKKKIFTLMKIAKHVIVCTKYLEEE
                     ALKHNKNVTDISSTFDTNRFIPLKEYEKRDVVTLGWTGSHSTMAFLHLLDEAIQEVAR
                     QRKIILRVICDRDFELEGVQIENIRWTEQNEVADLHGMQIGLYPTPKEEWVLGKSGLK
                     GLTYQSCGIPCVATAFGSNLENFEQGVTGFLADTTEEWVKYILLLIDDEKLRERIGKA
                     GRQNVMNNFSVEANKPKYLHILDSVVKQ"
     gene            complement(46579..47802)
                     /locus_tag="PLK75_00205"
     CDS             complement(46579..47802)
                     /locus_tag="PLK75_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37696.1"
                     /translation="MKNVLYISYDGMTDPLGQSQVIPYLQQLALCGYNITILSTEKPE
                     KFAQNQQFIRQLLNSSGIGWEIVVYTKNPPVLSTVIDIFKLRRKVKKLMQTMRFDIVH
                     CRSYISPMAGLMLKKKQGAKFLFDMRGFWADERVDGGLWNLKNPVYKTVYRYFKRKEK
                     QYLEAADHIVSLTHNAKSEMQQWDFVKFDAGNISVIPCCADFDFFRIPDTTQRNNSRK
                     KLGFTENELVLCYLGSLGTWYMVQEMFDFFSEVLHQYPNARFLIISGDWLNQEAQKAG
                     IEPGKITLISAKRSEVVGLLAAADIGISFIKPSYSKKSSSPTKQGEMLAMGIPMIVNK
                     GVGDVDSIVEETQTGIVIPDTTQEAYRMAAMQIPELLQKNKEDIRNRAAKILDLKVGA
                     ASYKNIYNTLTGDPN"
     gene            48020..49762
                     /locus_tag="PLK75_00210"
     CDS             48020..49762
                     /locus_tag="PLK75_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37697.1"
                     /translation="MRSLILISLVILGTMGIAQENTANYWYFGYQCGISFATGVPKGV
                     TGGQITGLEGCATVGDMQGNLLFYTDGQVVYDRNHNIMQNGTGLMGHYSSTQSSIIVK
                     CPGTENYYYLFTTDGAENHLEGGWRYSIVDMSLNGGLGAVTGVKNILLEGPVAEKQTA
                     IMHSNNVSVWVISHRWNSNEFVAYEITQEGISPVPVVSAIGAVHEGGPSGDPNLNGWT
                     NATGLLRANKEGDKIALAIQITGIFEVFDFDKSTGVVSNCKTSSAYNPVYGVEFSPDG
                     TKLYATVNVYQNSRLYQFNLNQVDPFSNSVLIASESDNPIKSLQMGPNGKIYITRYNY
                     GYVSAINNPNATGTACGYISNAVYLMGKTGRNGLPNIFYYKGFRFFTGSEQTISICEG
                     DSIYLEGAYQHEAGTFTDTLQTALDWDSIVTTYLELLPAPEIPVITEFNGVLYCTPAD
                     NYQWYRNGTPIAGADNQLYQPFVSGYYTVEVVWGNGCHSVSEGFNFIVQSIVSASDEK
                     RMIYPNPCGTSFSTDLEEQYSLKIFDIHGKECFTRENLQGLVFHDCSVLESGLYFVEI
                     QTHDTVIVEKMMKE"
     gene            complement(49769..51592)
                     /locus_tag="PLK75_00215"
     CDS             complement(49769..51592)
                     /locus_tag="PLK75_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37698.1"
                     /translation="MKKQLLILGILLSTTMAFAQFGGGSGTEEDPYRIYSKEHLEELQ
                     NSLSITVDYTGTHFSLMNDITDSLRTPIGRLNLDFNGYFHGHGHTIRVALDYRNDSYY
                     YTALFMILSPEAYVDSITVEGYIYAMCGIVNQNKGTVNSLINKTEYNYQNLNFLGTTP
                     NASGICRSNVTGAIVENCTNYATIHGYYLGGICCASAGSIINCRNYGNIKIYNSQGLN
                     NLNAGGICYNNSGSISGCKNFGSFEFTGQDLIVGGGGICGHANGPITNCQNYANLSGK
                     IDNLGGIAGSVIESAISNCSNYGIIDGRNNIGGIVGDNFGWSSSINNCFNSGNINGLQ
                     NVAGIFGNRNDDTITNCLNVGLCYGSAITCLDTTGLLTNNYYDKQMCLSKGIDSTDIS
                     GSAEGKLTTQLTGTSPELQAMLGDGWSYAEGRYPIPLGLENDSLALVAATPVYLHFDS
                     EEAYNHVDSVSKNFTVTTGISTGSTSVAFDWDETNGHISFSDENVTLLSLGVENLSVS
                     LGDYSKNVRINIVDIETSVPEQTIEKALRLYPNPAGTVCFIEDNKNIYGIIFFDLSGK
                     VVLDITEFLTDGINIESLMPGIYVVKIKTENGFAFTKLVVE"
     gene            complement(51796..52164)
                     /locus_tag="PLK75_00220"
     CDS             complement(51796..52164)
                     /locus_tag="PLK75_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY37699.1"
                     /translation="MERKPSQNFQDLVVWQKAHQLVLEVYRYSESFPKEEMFGLTSQL
                     RRAIISVAANIVEGFKKRGKPDKMRFINIAQGSLEECRYYFILAQDLKYGNSKHLEVL
                     AEEVSKLMESYNNSIKSSCS"
     gene            complement(52351..53439)
                     /locus_tag="PLK75_00225"
     CDS             complement(52351..53439)
                     /locus_tag="PLK75_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37700.1"
                     /translation="MKKKLLILSLISLIYTTSQSQNLLFWGEFVDGLIVRSNIDGTEI
                     DTLCTGQVGVCRVRTDDTQKKVYWAASYDNKIRRINYDGSELEDVINTANNINVVEIN
                     PHNGRLYFSENYQNTIKSCKTDGSDLQTVITNAGLVMGIGIDTVRELIFWADMAAHEL
                     HRANLDGSEDTVILETNLNVFDICLDIKYLYVYFTERTGEKVWRVSYTGTNLLEIHQG
                     SNVIGAITINDECNQIYWVERENGFIVTSTTMGAERQELIFWENSQISGIDVGNFDFS
                     NVEEDTFSLPDGVIYPNPAGDYINLNLNGIDADKIEIYDITGKLISNDNLSSEQTQFF
                     IGNLKSGLYVLKVYDGNTNIATLKFNKQ"
     gene            complement(53644..53850)
                     /locus_tag="PLK75_00230"
     CDS             complement(53644..53850)
                     /locus_tag="PLK75_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY37701.1"
                     /translation="MINYIREERKIRKLSQEELAKAMNVSRQTINAIEAGRYLPSLPL
                     AFKLSQYFEKTVNELFVNEVFDVE"
     gene            complement(53852..54286)
                     /locus_tag="PLK75_00235"
     CDS             complement(53852..54286)
                     /locus_tag="PLK75_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37702.1"
                     /translation="MFKSRIVFLPFVFRFIGLAIVLIALAMAILKLTNGTPFGTFFRF
                     DADIRVYAIHAILILGFFMIAFSKDHIEDELVQHFRTKAVFFSIMLHAVFFFVFTFTS
                     ITLQWINFPAIILMNSIIFVYILAFYILKIRENYRNSNNRYE"
     gene            complement(54367..57060)
                     /locus_tag="PLK75_00240"
     CDS             complement(54367..57060)
                     /locus_tag="PLK75_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37703.1"
                     /translation="MKHIFLIVSLIYTTSFVQAQCSNPYAGPDDNTCGVAYQLHAENT
                     TTGEWTAFHNATPLVVTFVGTAHASDPVAWVLIPFYSGLFFDVDFVWTDNSGPCADTV
                     RIRFAREPIAGAGPCDYVETCGNTYTFSADTVGMGWAYGTWISQLMSNFDNVHDPNAT
                     VTINPYGCFGDSASMQAQFLWVLNNFGCTSIDTINVHFYQVPTANAGYNYTICGLEGN
                     LHAFYSLEESSNYSPSGNWSVFSGPNLDVSFYAQNSNQTIVTVSEQGTYEFIWTETNT
                     NNPECLSKDTVSIQFLQIPVVDAGQDFNVCGNTTTLHATGTGYQGSWFNTPGANFESQ
                     TTPVTIVTSQYGPKMFFWQEANGICYDRDTVIVTFYPHPSAEILSTPADFDTLACGLV
                     YSQLMAEPASLGQSGHWVDTSNPFTMFNASSQPDFDTVTVTQYGYHDFYWIVETLVFE
                     EEPGFCADTAGPFTVNFLEMPVAHAGNDTMFCSLIGHLNAEPSEISGTWLYNCNGIII
                     EDNHNPVSPIITPQYSQECQFIWFLENESCNSSDTVVIKLVNHEIVQACSNQVVSSCT
                     PAYLNLETSETEYSILWEPEAGLNDATIENPTALPLVSTMYYVSITELLTGCVDIDSV
                     LVTVIPDDTISLCMVTVNSENKNLILWEKPVSDIIDSFQVFRESDGIYNLIGLSPYNA
                     LGEFTDVTSQPELQSYRYKISAMYNCGQETDMSDEHKTVWLNREIMSPTSWHLQIEPY
                     EGTEYAAIQIFRGTDPENLQWLTTLPADATEYTDNDVPTGSYVYYQAKIEITTPCNLA
                     KTGNSILSNIATNDPGFYAVSPVEFTQLTLSPNPALKTVSVSFDSPNAKLSICDLNGK
                     KLIVKNDFTGGEINVENLNAGVYVVKLETDSGIVMRKLIVE"
     gene            complement(57101..58516)
                     /locus_tag="PLK75_00245"
     CDS             complement(57101..58516)
                     /locus_tag="PLK75_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37704.1"
                     /translation="MKKQLAFVAMCLLFSVCTQGQTQIATSHCIDATANKNQRKIVRD
                     TLDNVYVVYHDSLDGNMVVKGLFLNRETETWSEPVLICNGKNPTFAISRGCKFFLIYE
                     TNDIVPRVAYRSSMDFSTWTEELIISEGSIPCRFPIADVDSAGNVHVFWQKIYNPSLY
                     DHLLEYARLVEDTVQKTELIEGVSDCAMACHLQYGNNTVYFAIGIGYDVDMSDAFTLL
                     YRSTNQMETWGDLPLQGVMFGTFATISYNTEYWYDMYHHDCTVKILYTDYDQQLVEKN
                     YIEQYNTNDERIIQSGPIDYHCIDDILPPLGYSYLFLRNNTLYHAFSYGYDTSPILET
                     IGNNPFNPSICYKHFRATVVDFIWMQEADEGYSIYYMRDDKFDPLGVVPIKELPIHLT
                     AYPNPFANSIRIEAVSPEPELEFRIYDINARIVHSEKLMTDGSGKYTFSWTPENEKPG
                     EYVVACFAGEHKAAGKIFFKP"
     gene            complement(58558..60891)
                     /locus_tag="PLK75_00250"
     CDS             complement(58558..60891)
                     /locus_tag="PLK75_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37705.1"
                     /translation="MSENSIITTCSGTLYDSGGANNSCQPGEHFIKTIYSGQANSHVL
                     INIEMIDIYMFDTLRIYDGPDTNSPPLQSNYAPVEENRSFISTGNCITIEWVSTYSYD
                     ADFAIGISCKYECIAYTNALFDAAGQLLPNGSYIDVCHNSTLEVSVESNFQSGQNGNY
                     PQSATTTEYTWLVYNDTMFLIDLFNEYSGMNLTDIAIPFDNPGVYMILLKSSDFNTCQ
                     SNNLPVLFVRVPPDPIFEITGHEEAACEGQEVQLTGYISPVTAVANDNDHIINNCIYG
                     CYEYYQHFPFYVSTSSNITTLQEPDDLSSLEVKMEHSAAGDLEMLLMCPSGKTVRIFD
                     ESTCGGKNYGIPTDDDNCVPGVGWTYYWSDEASISLQDIENTSYQNIPAGSYRPVQTF
                     DDLYGCPVNGEWQLIIMDNRCGDDGFIFSANIHLKGEYTDFLEIPEYPQNQWSSQSSD
                     NSFWTGYCLDGNSGNTTSFTPNFTGGGFPYVFHITDNFGCVYDTTIILFILDKLDPQC
                     CPLPEPHICYVTSSGLYGNLIRWGMPQAALDSVYIYREEDGEYHQVAACDYAQAQFTD
                     YSAQPWAASSNYKISFINNCSEETVLSSNHRSVYLQVNSPNESTCQLIWNEYFGLDYS
                     VTEIYRGISPYHLELLTSVNNDVGTFTDDNLPEGAEYCYYRLGFNTTADCSGAKDDVM
                     IFSNIATNNPGFYGMGSNASPLQFEIYPNPTSGNVSIQFDTPKASLSICDLNGKVLIA
                     LNDFAGGEINIEHLKAGVYVVKLETDGGVGVRKLIVE"
     gene            61168..66147
                     /locus_tag="PLK75_00255"
     CDS             61168..66147
                     /locus_tag="PLK75_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOY37706.1"
                     /translation="MFRILLLPLCLIPSLLMAQFTDDFSDGNFSANPEWTGNTDKFQI
                     DASNRLQLNEIEYTGSAYLSTASQAVNEASWEFLLQFDFNPSSSNYARIYTISSGSDF
                     TGSLNGYFIRVGYTDDDISFFRQDGAAASLLIDGTNGRLNSDAVTVRIRLERDGLGNW
                     SLFSDTLGGTNYYAEGVCFDNTYNASNYFGVRCTFTTTRKDKFFFDDFVVTGTPYTDN
                     DPPVFQSVTVISENSVRVVFNEDLSQETALQFANYSVDHEIGNPVSASFFNDNHAIVD
                     LGFANDFISGTEYTLQYSGVEDVAGNVVVSGSGTFIYLITQPGMVVINEIMADPNPVV
                     DLPEAEYIEIYNTLQVPVNLTGWRLIVNNYVRTFPAVEIGPESYLLLCSTTNVELFGT
                     EIPVAGISSFPAIPNDGATLKLFDADSVEVNQVTYDVTWYHDATKDDGGWSLEKIDPL
                     NNCSGATNWIASEDPSGGTPGFINSVNAVNTDNTPPEVAMVQLSGTTEIVINFSEPVD
                     SITATDPQNYMISHGYDSPDNVETDATDPAVVHLFVQTAFSEGIVYSVSIEGISDLCG
                     NTMLPSQHDFSIYTPAVNDIVINEIMADPTPVVGTPEAEYIEFYNTTENPVTLTGWSL
                     VVGTYIRPIPAAQVEAHGYIVLCSTGNTNLFNTGVNVVGIASFPAVPNSGQTLKILDP
                     NGNNINIVSYQDTWYQDPSKDDGGWSLEKIDPLNNCVEMANWRASVNPLGGTPGAINS
                     VYASNPDNTPPHIQALQVINNAELLLEFSEKVDSNTILISNFLVEQLSVHPTQVLLNS
                     DSKTVSLTFADPFEENTIYTLEVSNVADLCGNAMETVFSEFNIYFPVVNDIVINEIMA
                     DPSPVVGLPEAEYIEIYNTTANVITLTGWKLIVGNYVKEFPSAQIGANGYMVLCSPAN
                     AVLFESGINTLGITSFPALPNDGKTVKILDADSVEINQVSYDISWYQNSDKIEGGWSL
                     EKIDPLNHCGGMSNWISSVAEIGGTPGAVNSVMAENPDSSPPTVVSVQALTGNELLVT
                     FSEFTDTILAVNPANYQISPDYNNPEYVYVDPNDLRQVHIFVPYSFTEIVVYTLTISN
                     ISDLCGNVMATSNINFSIYNPQPYDVLISEIMADPDPVVGLPNAEYIELHNVTDMPVN
                     LAGWKLFSGSSSCVLPAYTIQAGAYITICHTNNSLLFAQYSNVLGVSGFPALPNSGAV
                     LSLVTDNNMNIHAVEYSDIWYNDNFKKAGGWSLEMIDPSNPCEGEDNWIASTSNRGGT
                     PSEMNSVIAENIDVTDPFAESAEIVNADTVRVYFSETMRISSVLPVLNYLVDHSVGNP
                     VWVYPEPPLFNSVLLKFNTVFEPGVVYQLQFADTLRDCRGNDIVQTFEVLFGIADSIG
                     QGDLVINEILFNPYPDGYDFVEFYNNSGKLLDLRNVWLSQRDDSGNPGEPDRASERSH
                     VILPDDYIVITDNPADIATRYYVPSPQKIIYSDNMPSLPDSDGNLIVFDRAMNIIDEF
                     SYDADMHFELLADDDGVSLERIDFVEPTQAASNWHSASGDVGYATPGYQNSQYRPSSV
                     DAINSLVPDPRVFSPDNDGYNDRLNILYQFENAGTVATIAIYDSKGRFITYLAKNESL
                     SAQGVLFWDGLDTRNQICPIGIYIIYAEMYDATGKTWNEKITCVLSIKK"
BASE COUNT        19656 a        13268 c        13672 g        19815 t
ORIGIN      
        1 atatgctttc ggagctgata tcaaaagaaa agaacaatgt tagtgtaaac tttgataaac
       61 ccgaaagtgt aattgaagtt attaccgacg aaacccgctt gtttcaggta atgctgaatt
      121 tgttgaataa cgcggtaaat tttacagaaa aaggacgtat tgattttggt gtaaaaatca
      181 tgagcgaata tttacagttt tatgttaaag atactggaat tggtattgca aaagaacttc
      241 accaaaaaat atttgaacgt ttttaccggc attcggggtc cttgtcaaaa actcaccgtg
      301 gcacaggatt gggactcacc atttgtaaaa gccttattga attgatggga gggaatataa
      361 tggtggattc tgaactcggg aaaggcactt gcatgagctt tactattcca tatctgaaac
      421 cggtaacttc tgtggaaccc ggcacgtttc gggtagttgc tgctaacccc gactttgcag
      481 attacaagat tcttgtggtt gaagacgatc aggcaagcct ggaatacttg cttgctgtat
      541 tacgaaaaat aggattcaga tttataaata ttgcacacaa tgggaatact gccgtagaag
      601 ctgctattcg ggaaacatac gatgtaattc taatggacat tcaattgcct gatattagcg
      661 gaattgaagc aactaaaagg attttgaaac atgaccctgc agcaagaata attgcgcaaa
      721 ccgcatatgc aatgcaaaac gataaggata cctgcacaag ggaaggttgt atcgactaca
      781 ttgctaaacc cattaagata aatgagctta gccttgtttt atcgaaacac cttgttaaat
      841 cgttgtaata ttaagtagag catattattt attcaacaat attcatctcg ttgataagat
      901 tctgagcatt ggcatattta tcgataataa ataatgtgta acgcacatca agattaatgc
      961 atcgctgaag attttcttca aaatcaatat ctcccgacat ggcttcgctg ttaccgtcga
     1021 aagctgttcc aataagctct cccttaccat tgagaacagg tgatccggaa tttccaccag
     1081 tgatatcgtt gttggtaata aaaccggtta ccagcttgcc ttcggcattg gtatacctgc
     1141 caaaatcgtt gtttttataa agttctttaa gtttttcggg aacaataaac tcatcattat
     1201 tcgggtcttc tttctgcatt acaccttcaa gggtagtgta gtacttatag ttaattgcat
     1261 ctgcgggcct gtacccaaga acattaccat aggtcagcct aatggttgag ttggcatcag
     1321 ggtaaaacat tttatcggag ttcatttcca tgagtccttc gttgaagagc cgtatttctc
     1381 tttcgagtcc gctgctctta tcgcggtacg attgatttat ttcctgatat tttgtataaa
     1441 tggagttccc tgcaattaca gcagggtccc tatccaggat ttttgcgttg ggagcatcca
     1501 ccaaagcaag gagcttctgt tcgtcggaaa ataccgattt caaaaacata tcggcagcaa
     1561 atttatcgaa attaccctta tactttttaa taatggtaag aaaaacagtt ggatagttca
     1621 ttgggtcaac attggaagca taatatgaga acatggctgc aaccagtttt ttatcagtat
     1681 tggcatcgaa atttttataa aagttatgaa tactggtttt gattttttcc ttatcggctg
     1741 cagtactgtt ttcatttagc ctaccaatct ggttgcttat ctgatatgca aataatggta
     1801 tttcggcacc ttgtaacagt gcttcgagaa taaactgccg gttgtaagca tcggctttac
     1861 gcgattcata aatgttttta atattgttca atacattccc gtacttagct ttacggttgt
     1921 tatcagcatt tgtccaagct acaaattcat tttcaatcgc ccttttgttt tcgatagtat
     1981 taagtttctg cagggcaatg ttttgctcat ggctgtactt ccagtagttc gaactgctgg
     2041 catatttcga agcatactgt attcgcactt cggggttgtt tttcatctcg ttgcggagaa
     2101 tgtccagctt tacagtgcgt atattgtacc tgtaagtatt agtgatgtca attgtttcct
     2161 gaagaccgta agaagttaag taccgatcgg ttgttccagg aaacccaaga atcattgcaa
     2221 aatcgccttc tttcactccg gcaatgctaa taggcaagga atatcttggt ttaagcggaa
     2281 tgttttcttt tgcataggca gcaggttttc catcgggtcc ggtataaata cgaaacatcg
     2341 aaaagtcagc tgtgtgcctt ggccacatcc agttatcggt atctccgcca aattttccca
     2401 tcgatgacgg tggagcacca acaaggcgaa tatctttgta aatttcgtat accagcagaa
     2461 aatactgatt attatgaaac atttccgaaa cctgagcact atagtacgtt cctttttcag
     2521 cttctttaat aatttcgctg gaagcttcct ttattgctgc gttcctgtcc gcctcaccca
     2581 tctccgatgt tacgctcttc attacacggc ttgtaacgtc ttcgatacga attaatattt
     2641 cggcacaata accttcgttt ggtaattcct gatctttgct atatgcccaa aacccgtctt
     2701 tcagataatc gtgttctatg gatgaatgcg cttgaatcat accgtatccg caatggtgat
     2761 ttgtaagaat cagcccctgg tcggaaatga tttcgccact gcaaaaatgc cagaaaggcc
     2821 tgccttcacg gcttaaccca actattgcgt ctttaataca ggcattatta atgttataga
     2881 tatcatccgg tgtgagttga aaccccttgg ctttcatatc gtcataattt ttcccgatga
     2941 gcgacaaaag ccacataccc tcgtcggcac gtaatgttcc cgaaatcagt attacaaggc
     3001 ttaccagcaa aaaagcaaat tttttcattg ttttgtgttt tgtaaataat gaatcaaaaa
     3061 taatgtctaa atctattaaa acaaacgatt atctttggtt taagattttt ttaagttatt
     3121 ctcaatgaaa gttgtcttta ttttattttt ctgctttgtg ttttcaataa tgggtttctc
     3181 acaaccggaa ccggtactta ctcaaaacga cttgatgcaa accaagattg agcgaagtgc
     3241 tttacaatat atgaaaatta atttattgag gacgtatatt atggaggttg aaaaaaatga
     3301 gcttaccggt agaaaagctt tggtatcgga ggtatattac gacaaaaacg catgccctga
     3361 atccattgta tatttcgata gcgctggcaa caggtcgcaa tacactcatt atttatataa
     3421 ctctgattta tacaaaacgg aagaaatctt ttttaacagc gatagcaccc tcatcggtgg
     3481 cgtagccatc gaatgcgata gttctggaat taccaaagcg tttattgttt acaacaagaa
     3541 cgcagaaatt acttcggaat ataaattcct ggtagatcct gtaaagaata ttattgttgc
     3601 ccaaataagc aataagaaga ataacaagca aaactacaag tattatcata taaccggagg
     3661 catgaaagat ggccggattg cttcggttga aaacttcaat gaaaatggaa tattaaacga
     3721 cagtataagc taccattaca atcaggcggg tcaattgtcg ttcaagcaat atttcgattc
     3781 cgacaggaaa gcaatgcagg aaacatggtt cttttataac gatgaaggag ccgtggaaaa
     3841 aacacaggag attaatattc tgacttcaca acagcgatct ttttcttatg catacgatga
     3901 gtatggcaac cgcacaagct tgattgaaag cgataaaaac aataaaataa tcagttatta
     3961 ccgttttgaa tacttttcca gagttctccc tccagaaaaa agatgataat tcgtggttat
     4021 ttcctgagtt tataaaatct ggaaattata agtcctgtga agaaaataaa accttctcca
     4081 atcaaatacc acagccgtga acttaccgaa acggttaccg aaaaatcgga agccagcccg
     4141 gcagcaatca aggtcagcga cagcaggcct tcacgcattc caagtccacc gggcagtata
     4201 atggcaatca ttccgagtga agcacccaaa gcatagaata agcctgcaaa taatgggata
     4261 tgctcgggaa caagagaaat tgccaaaaac caaaagccca aacaccatgt gagccaacac
     4321 aaaactgaga aaggtaagac aaacaggacc gagctaattg tgagttgagg tatgtcgaaa
     4381 cgctttcgta acagtttcat catgataact gtaaacaacc tgtgaaataa cggcgtaaaa
     4441 agaatgagac tgaaaattat aagtaaggca atactcccaa gaacaatata ccaagagacc
     4501 tgtataaatg ccaggcccaa tataccaagt aaaagagcgc tccataatga aacaaactgt
     4561 acgttaagcg aaatggatgc cacctccttt tcattcaaat cgtatctttt ggaaatataa
     4621 gccgaccgcc caagtattac ccaaatttta ccgggaatat atttgccgaa aatcgacaac
     4681 ccctcgcttg ctacacaatc caaatacttt acttgtttgc agttaaataa atgtaaggct
     4741 ttgtaccaac tgtaggcatc cagaaaaaac cctataaaaa caaaaacaaa tgccagaacg
     4801 agatgaaata cattccttac aaccggcacg acaaaataat cggccctgac aaggaataca
     4861 atcagaaaaa tgattgatat ataaataaag taattaaacc attttttcat aatccagcct
     4921 ggttactact tatataagcg ctcattatct tgtatatcca tccacatggc aaaaaagaac
     4981 gactgtaatg acgaaataaa aagaaaaata aaaataatca ggtaacttat gggggtaaca
     5041 tcggtagtga acatgttgta aacaatgtta atcagaaaag gtatgtttgc tattgaaagc
     5101 aaaaaagcca gcatgtagaa taagaaaagg gggtgaaaat cacggagaaa gtattttcgt
     5161 accaagcgcg taaaaaaaga acgaaaaaga agccatataa ttctaggaat taccttgtta
     5221 accttcattt tagaaacttc ccccacacga taaaccggct taatttcaat ttcgcggata
     5281 ctggatgacg caatgttaag ttttacaagc atatcgttag gcatgccgta cttggggtaa
     5341 atattgtaca gttttatcga tttcagcgca tcaagcgata tggcagtata accggtttgg
     5401 gtatcagaaa cgcgccaata tccgctagat aattttgtta gtatcgaaag taccgaattc
     5461 ccgaaatgcc gttgcttcgg aatcacgaat ttcgcacttt tatgcatcaa tcgatttcct
     5521 tttacatagt cgaggttttc ggcaacgaca ggtttgcaaa tcatttccag ttctgacgga
     5581 tccatttgtc cgtcgccagc catcaccgca gtgcaatcaa tttcattgtc tttgcaccat
     5641 ttatatcccc ttgcaattgc tgctccaacg cctccgtttt ttaaatggtt gattagaatg
     5701 atgcggtcat tttcaggttg tgtattttga attgctgagg gagtgaaata tcgaatctca
     5761 tctttgaaaa tttgctgcaa tacaacgtct gcttcattgt agcggtttgg ggttactgtt
     5821 ttttccgcga tacggggatt ataataattg cccgaatcgg tatcgatata tgtttttacc
     5881 acatcggccg tattgtcggt tgacaggtcg ttgactacaa caattctgtc gacaaaatcg
     5941 ggcatgcttt cgatgacctg aacaatttgt gtctcttcgt tatatgcagg tactacaaca
     6001 gcaatggttt tattaaatag cattttttat tttgtttatg caaatgtgct tattatttac
     6061 gtcctgtgca aatagcattt ggctgacaac ttgtgtgagg catataaaat aaccgctcgg
     6121 caattgatac ggaaagttta tttacatgag gcaaatttaa tagataagca aatgctcatg
     6181 gcaaaacaaa ttcttcaatc atatttgctt aatattcctt acatttgcgg ttgtaaaaat
     6241 gctaatatgg acctgttgca gataaaacaa cgttttggaa ttattggaaa cgcacacgct
     6301 ctgaatcggg cgattgaggt ggctgttcag gtggcatcta ccgatttatc ggtgcttatt
     6361 accggagaaa gcggtgtggg caaagaaatt ctccctcagg taattcatta cgaaagcaaa
     6421 cgtaaacaca actcctatat agctgtgaac tgtggggcta ttccagaagg gacaatcgac
     6481 tcagaactgt ttggtcatat taaaggatct tttactactg caatcaacga ccggaagggt
     6541 tattttgaag aagccgacaa gggatctatt tttcttgatg aagttgccga attgcctctt
     6601 tcaacacaag tgcggcttct cagggttctt gagtcgggag agtttattaa agtgggttcg
     6661 tcaaaagttc agaaaaccga tgtcagggtt attgctgcaa ccaatgtgaa tatcatgaat
     6721 gctgttcagg aaggtcgctt cagggaagat ttgtactacc gtctcaacac agttcctcta
     6781 catttacccc cactcagaga aagaggagaa gacattgcat tgttgtttcg gaaatttgca
     6841 gccgattttg ccgagaaata tcgcatgccc ccaatacgca tcgacgatga tgcaaaagat
     6901 ttgttattaa agtatccatg gccggggaat attcgccaac tgaaaaatat caccgaacaa
     6961 atttcggtaa ttgaacaaaa ccgattgatt gatgcggtta ttctttccag atatttgcca
     7021 gctatctacg ataaccgttt gccggcacta ttgtcgaaag tagatgagaa aaaatttaac
     7081 tccgaaagag aaattctcta caatattctt ttcgatatga aaaaggatat gaacgatcta
     7141 aaaaagctgg taaatgaaat tattgcacaa acaccccacc tcgaccttca ggagcatgtg
     7201 catgatatca caggggagtt tgtgcaagat tccgatgctg aaatcagaat tgaaccccgt
     7261 gtagttgtgg ccaacacaat tcagaatttg caacataaaa attttgaaga taccagcgaa
     7321 attgttgaag agaatctatc aatcaacgat aaagaggttg aacttattca aaaagccctt
     7381 gaaaaaaact cgggaaagcg aaaacttgct gcaaaagagc tcggaatttc cgaaagaaca
     7441 ttatacagga aaattaagga atacaatatc gatgcctgat gaaacaaacg caacccatat
     7501 ttcaaattat tatggttgca gcaattgttg cagtttttgc ttcatgcaaa gggagttact
     7561 cgtttacagg tgcaagtatt tcgcccgatg ttaaaacaat acagatttcc tatttcccca
     7621 acagggcttc caatgttaat ccgtcgttga gcagcactct taccgaggct ttaaaggaca
     7681 gatttctgtc gcaaacaagc ctaacgatag tgaactcaca gggagacctt actattgaag
     7741 gtgaaattgc cgactatcgg gtaacacctc aggcatatca gggcaacgaa acagccgcat
     7801 taaaccggct tacaattaat gtcagggtga aatttaccaa tacaaaagac ccatctaaaa
     7861 gctacgatac gcagttttcg aggtatgccg attttgaaag ctcaaagaac ctggcaagtg
     7921 tcgaatcgca actgattacc gaaattgtgg atcagcttac tgaggatatt ttcaacaaag
     7981 cggtggtaaa ctggtaatgt atgaataaag aagcttacag atcgtacctg cagaaaccgg
     8041 cgctaatgga ttttaacagc ctgagcgata tggacgcttt gttgaaggag ttcccgtatt
     8101 ttcaaacggc atggttgctg tacgcaaaaa atcttcaact gattgacgat gtgagattgg
     8161 gaagccaggt gaaagctgct gcactgcaca ctcccgacag agccatgctt tacagattac
     8221 ttcatgaggt tcacgaaaaa aaatcagaaa aatacggatg tcaatttgct aaaacctctt
     8281 ctatagaaaa aaacatcatc attgactctg caaacaagac cgatatttcg cagataataa
     8341 tctccgatgg gttgctcgat tttggcttta tgcgaattga attcgctccg gaaagtaaat
     8401 tttctgcatc gttacctgcg gaacaaagca ctgtttttga gagcaacacc actgaacccg
     8461 atgtaaaaga tagtcgcata aaaaataaat tgctcatcga tagatttatt tcagataaac
     8521 ctaaaattat tccgaaggaa accgaaatag cgttgaatac tgatcttcct gatattgaaa
     8581 gaggcgaaaa cgaagagctg atatctgaaa cacttgccca gatttatgtt aaacagggct
     8641 attttgagaa ggcattgcgc acatatgaaa aattatcttt gaaatatccg gaaaaaagca
     8701 tttattttgc aactcaaatt aaaaagattg aagaactgtt agataaaact ttataaacat
     8761 gtacacattt cttaccgttt taataattgt tatttgtatt cttctgatac tcattgttct
     8821 ggttcagaat tcaaaaggag gtggacttgc ttcaaatttc ggacaaggta accagattat
     8881 gggggtgaaa aaaacagccg attttcttga aaaagctacc tgggtacttg ccagtagttt
     8941 gctggttatt tgtataatcg ccagtataac cgtgccacgt aagccaaaag ccggagaaac
     9001 gaagaaacaa actactacag aactacctgc atcaaaaggc agcaaatagt gtgaataatt
     9061 ttatggcagc cacttcgctt tgctgtaatt gttacatttg ctgatacgta attcaagcaa
     9121 atgttatggt tcttaataat ataaaaagta ctttaaataa tcggaaaacc gaatttatta
     9181 tatttacttt ctttgtatta ctcaatagtt cttttctgct ctgctgcaac aacattcact
     9241 ctctcgacgg tccccagcat ctgtataact caaatgtaat ctgcgaactg ctgcaaggaa
     9301 acaacacggt ttccgaatat ttcagcctca atcgcgtgcc cgtgggatac ctaacaggac
     9361 ctgtaatact gtcggcgtta aacgcgttgt ttccggcaaa tattgcgctt aaaattctgg
     9421 tttttatttg tattgctggt cttgcgtact catttcgtta catggtttat tcaattaata
     9481 aggttacaac ctacactact ttgttaatta tcccgttcac acttcaaact tttctgggaa
     9541 tgggctattt tacattctca ctctctcttg ctttttactt tatatgcatt gggtatgtaa
     9601 tccgcaactt tgattcattg cgttttaaga acctgtctat acttggtttt ctgctcctga
     9661 tgacatattt tactcacatg gtggtgtttt cttttaccat gggaaccata gccttatttg
     9721 ttgtttttag gtttttacgt gaaatgattc aacgtaatca aagcgtgata aaaagatacc
     9781 ttattatcgc catgaaaatt gcaatagcat cgctgccggc tgttgtgctt gctgttatat
     9841 acgcctacca tgtatttaaa ttgaatacac caatcccaaa ccctgttatt actactactg
     9901 aattgtatcg taatgtattt attatgaaga acctgattgc atttgactta acagccgaag
     9961 caatcatggg caaaaccata ttttttgttc ttattgcagc cttcagttat gtattgattg
    10021 tgcttttaca ttctgttatt acgaaaggca aacaattatt ccggagtgga aatggaaaat
    10081 ctgaccgatt gttcttcttc tgcgtggcgg tcttgttttt tataatgtat gtttttatgc
    10141 cgaatgttgt tggcaccgga gctgttagta tgagagtgct cgtgctattt tatctggttt
    10201 ttgttttgtt tttggcatta cagaagtatt cccctgtgtt tttagtactc atgctaggtg
    10261 taatattcta ctcttctgtt cagcgcagtt atattgcttc cgacagcctt aaatcactaa
    10321 gcagggatgt tgacgaaatc atggaaatga atgattatat ggaacccaat tccacatatt
    10381 ttgcaatgaa ttattctaca tattggcaac atagtcatat cgactgttac attggtgtcg
    10441 acaagccact gatcaacctt gccaacccgc aatgcctcgg acatttcccg gtggtgtggg
    10501 aaaataaact gccctgctta tatcttggca atcacgcatc acacgaacta ggcgtgttct
    10561 ggaattctgc cggaatagat aggaccatac atatcattga ttattttgtg gtatggggat
    10621 atggctcatt tataaatgaa aaaagctcac taaaaacaac tatcgacaat tactatgaat
    10681 tggttgctgt aagcagtcgg gggaatgcag ctctttatag atttaagttg ggttccctta
    10741 ttgatgaatc gcttgaaaaa atgaaaaaat caccggaatg gctggcacag ttgcagagaa
    10801 aagcccaaga aaagcaaatt ccattacaca acgcaatgct gttggatata ttttatttga
    10861 tagattatta caacttacaa gcaaaagata ctaactgata ttcaataggt taagtgatgg
    10921 atagcaacaa tattaaattc ttgattatac ggttcagcag tatcggcgat attgtgctta
    10981 ccactcccgt tgttcgctgt attaagcagc agatagaggg tgccgaagtc cattatctaa
    11041 ccaaaccaca atataaattt cttctggagg aaaaccctta catcgataaa attcatgtac
    11101 tcgggcgttt ttccgaaacc ctcagccaat tgtacgatga acatttcgat tatataatag
    11161 atttacataa taacctgaga agcttgcgat tcaaacaccg gctgaaagtg attgattttt
    11221 cgttccataa attaaatcgc gaaaaactgc tgctgacttg gtttaaactt gataagctgc
    11281 cagacattca tattgttgac aggtattttg caacagtctc tttattcgat gttgagaacg
    11341 acggcaaagg gctcgattat tttttgccgg aaaatgaccc taagaaagta cacccattac
    11401 tcgaagatct tcctttggtt tattttgttg ttgctattgg cggacagcat gaaacaaaga
    11461 aattgcctcc atacaagctt gccgaacttt gccggttgct gcaatatcgt acaatattga
    11521 ttggtggcaa tcaagattcg gaagctgcat cagaaatagt tgaaatgaca gagggtgccg
    11581 atgttacaaa tctttgtgga cttattagtg tgaacgactc ggctgcacta atcaggcatg
    11641 ctgctgttgt aataacccac gataccggaa tgatgcatat tgcctcggca tttcgaaaaa
    11701 ttgttttttc gatttggggt aacacaatac ccaaatttgg gatgtatccc tatatgcccg
    11761 atgaacgatc gaaaattttt caggtggagg gtctgccctg tcgtccttgt tcaaagctgg
    11821 gccgtaaacg atgcccgaaa aaacattttc ggtgtatgac cgagcaaaat gtgaaaacgg
    11881 tagctctgga agcaaataaa atagcttttc aatgttaaaa acctgtccac ggtgtaatcg
    11941 gagatttcaa tgcacaggcg attataaatg caagtgtatg aacgtaaaga caaatccgga
    12001 aaaacttgcc gaaatacgta aaatgtacaa gtcatgcctt tgcctaaagt gcttgcaagc
    12061 gttaagctca aacgaaaatg agaatgattt gcataaataa tatttatttg tatttttgca
    12121 tcacccaaag ggtaacttgg ccgagtggct aggcatcggt ctgcaaaacc gggtacggcg
    12181 gttcgaatcc gccagttacc tcagaaaaaa cctgaaatgc ttcaggtttt tttgtttaat
    12241 caagtttgaa tatcagtgga attacatacg atactttcac cggaactccg aattgttttc
    12301 ccgggctcca ttgtggaagc atactgataa cgcgtactgc ttcctgatcg agcaatggat
    12361 ctacacttct gataactttt ggagttgtta cccagccgtt ttcatcgatt acaaagctta
    12421 catataccgt tccctgaatt ctattttcct tggctactat ggggtaagca atattgtcag
    12481 aaataaactt cagcattgcg gcttctcctc cgggatattc cggttttact tcaacactaa
    12541 aataatcgga aataggttct tccataatca aagcatcaga ttcaacaact actaaatcat
    12601 gtgttgccga gggtggagca ctctcacgat ccgagtcttt tttatcgaga gcacttgatt
    12661 cctgtctttg gtgctgcttt tcctgagttt ttccagttgc ggtcgtatta tcgctatatg
    12721 caaatccccc cgcattcccg ttaacctgct tgttgttcga atcggcaatg ctatgttcct
    12781 cgctggcatc accggacccg ctcgcatcgt ggtcgtcttc atcaatgtta aataactcat
    12841 cgaatacagt tttctcttcg ggttcggcat agtttcttga ttcggaagca gaggcagggt
    12901 agggttcggc ttctttaatt ggcgcatcaa tcttttggcc aggtatgttc tgggctaact
    12961 cttcatttgc agaagtttca ctgcgaagct tatcatcaga ttgttccttg ttctttgata
    13021 aagagttttt cgaaaggtcc gatggtttat ataaggcttg ttttgaatcg tagttcatga
    13081 aaattaccac acatacggca aaggttacaa gaactgctgc tgctgcaaac cggagagagt
    13141 aagtgctaat acccagaaca ttgcggtttc gcttgtggag tattttttta tcaatctcac
    13201 gattgatagc gagaatttct ttcttcacat cgtgattatg cggcattgcc tgcaagcctt
    13261 caaccacgtc ggagcaaacg tcgcagtcaa ccagatgcga ctcaatagcg tgcgatgctt
    13321 ctttcgggag ttttcctgca acataattgt cgagaacctc gaagctgtag cagctgcttt
    13381 tcgaccatat ttcggattta tttgccggca ttgtaatttt caatcaatat tttcaaattt
    13441 cgtttaccat tttgaatata actttttacc ttttttaaat cgtatcccgt ttcatcgctt
    13501 atatcaacat acgacatctg cttcagataa aacaactcga cacaggtttt ttgttcaata
    13561 tttagttttt cgatacatat ttcgagacat acaagttttt tttctctttc cacgtaatca
    13621 agatgcaaaa atgggtcttc ttccataaaa ttatttgatc catttttcaa tgtatcatca
    13681 aacgaataag agcgggtctc ttcgcgaagc atcattaaac attcatttcg aacaacggtg
    13741 tgcaaccacg atttgaaatt gacaacctga tgggttttaa gtttctcgat aagcaattca
    13801 aaaacatcca ttgccatatc tttgcattta tcgctatctt ttaagtactt gtagcttatt
    13861 gaataaatca acgatgtata tcttttgaac aattccccga taatttcagg cttgttgttt
    13921 tgctgataca aagcgatcag cacttcgtcg ggttgatttt tatttagttt aataccaaac
    13981 acacacttat atattgcagg ctaatttaag tattatatcc ctgatttctg agggaaaatc
    14041 ataaattatt taacagagat tgtcagttat ttatttcatg gaaattcact aattttgaaa
    14101 tatgcaagct cctgaagaac gaatacataa atttatcgga aaacaccatg tctttacgct
    14161 tgcgaccaca gttgcagggc aaccttggtg tacttcatgt ttttacgctt actggcaaaa
    14221 tcgggtaagc cttgttttta cttcggagca acacacaagg catgtgaatg aatttacgaa
    14281 aaacaagcag gtggcaggaa ccattcatct cgaaacaaaa attgttggca tgatcagggg
    14341 aattcaattt atcggagaag ttgtcaggcc tgatgaggcc gaaacggcca ggctaattta
    14401 cctaaaaaga ttcccttttg ctgtatttat ggattcagaa ttttgggttt tacgcattga
    14461 ttctattaaa atgaccgaca accgactcgg ttttggtaag aaaatacatt ggacacgaga
    14521 tgaaaattaa cataaatcaa atattcaacg attatgaatt gatcgattgt ggcgatggag
    14581 aaaaattgga gcgtttcggc aacaaaataa ttataagacc cgaagtgtct gctaccggaa
    14641 aaccgtttct tacaaaagct gaatggcaat ccttagcgga tgctatttac cttgataata
    14701 ttggcactgg tggtaaatgg agctttttta atacgccgtt tgattcatgg gaggttatgt
    14761 attcagtttg cggaaaacct ttaaaaatca gattgcagtt aagtagtttt aagcatgttg
    14821 gtgtgttccc cgaacaggca ataaactgga attttattgc aggattagaa ggattattcg
    14881 gaaggaaact tcaaatttta aacctttttg gctacaccgg agtggcaagt cttgtggcag
    14941 ctggcaacgg acacagggta acccatgtag atgcgctgaa acaggtggtg cagcgaggaa
    15001 agacaatgat ggtagaaaat aatcttgatg gtatcagatg gattaccgat gatgtgcttc
    15061 gatttgttgc aaaagagcag aagaggggga attcctacga aggcgttata ctcgatccgc
    15121 cggcaatagg aaagggccct ggtggtgaaa tatggaaact tgaaaaacat cttgaaccat
    15181 tgttggctga aatacgcaag attttaggca atcgtagttt tgtaattatt aacctgtata
    15241 cgcaaataat taatcaacag gatgccaata aattagtttc agattatttc ccggattttg
    15301 ttaccgaaac cagcgacaga ttactcggtg aaagtcgttt tggcagcagt gttgattatg
    15361 gtattgtggt aagattattt aggaaatagt tttacatggc ttttagcggg cttttaacag
    15421 tgccatcatc gtaaagcaca aatcggtgag tattattctt gcgtttccgt tcctttcgat
    15481 atgataagca gctttttcaa atgcctcagc aattttttcc acattatttg gggtgatata
    15541 ggctgaaaat cgttccgaaa aatccctctc ccgttttaca aggaacgaca tttcggggag
    15601 tgaattattg attagaaaat tcccccgaat catgcgcagt gaatactcca tcaggctttt
    15661 ctgcttctca cgaccggtcc ctgcaatttc tcccacccac ttcaccaggt catatatgtt
    15721 gcgggaataa gcttgtcgca tcagtgcaac aaatttttcg aaatagaaga ctagctcttc
    15781 tgaagtgtta atatgattta cagcttccag atagtttcct tccgacatat tgcatatctc
    15841 acgggcttca gaagtattta tgccgaactt ggaaaccagt gcttccgaaa ggcttttatt
    15901 atcgattctg ggaattttta cagggagagt ccttgataaa atggttggaa gtatttcttc
    15961 gtgattatcg gctaccaaaa caaatagggt tttatcggga ggttcttcca gcattttaag
    16021 cagtttattt gctgtggagt tattcattcg ttccggtagc catattatca ttaccttata
    16081 ttccgcttcg aaagttttca agtttagttt gtgaagaatc tcgcgagcct cttcagcgta
    16141 aatactgccc tgtttgtttt cggctccgat gcactccaac cactcgttca gggttatgta
    16201 agggttcgat agaaaattga ggcgccacga ctcaatatat gtgtcgctga tactggattt
    16261 gccctgcgcc gatataacgg ggaatacaaa atgaaggtcg gggtgaatga gttttgcata
    16321 ttttctgcaa cttgcacatt caccgcatga atcagttgct gatttgtttg cacaacaaat
    16381 gtattgggca taagctacgg caagtgctaa tttcccacta ctgggcggac catagaacaa
    16441 ctgagcgtgg ctgacgcgat tttctgtaac tgtttgtatt aaatattttt ttacctcttc
    16501 ctgtcctata atttcttgaa atttcatgtg cgaatcttgt ttgtgctaaa ttacattaaa
    16561 gagatgaaac aaaaataatg tttgcacttt ttgtgaaaaa tagcgcgcta aacataaata
    16621 ttacatggtt attttcggaa tatattacta tttttgaata gatgtaatag cagtccattt
    16681 gtaaaaatta aaaaaacttt aaacccacca ttgatatgta caatcttgat aattacaact
    16741 ttacaaacaa aaaggttttg ctgagagtcg attttaacgt gccaataaat gcaggggtta
    16801 ctccaaccga tgatatgcgt ataaaaacag cattgcctac aattactaaa ctgcttagcg
    16861 aaggtgcagc aataattatt cttacacacc gtgggcagcc gcagggtgta aggactgaaa
    16921 gacacagcct tgtgcatatt ttaccattgg taaaaaaact aattggccgc gatgttaagc
    16981 ttgctgatga tgtaaatagc gatgaaacag ccgagatggc tcgcaccctt aagcctggtg
    17041 aaattatgat gcttgagaat ctgcgttttt ataaagaaga agaagccgga gatgatgaat
    17101 ttgcaaagaa actggcctct ttgggtgatg tgtatgtgaa tgatgctttt ggtaccgcac
    17161 accgcaacca tgcatccacc gcagttattg ccaagtattt cccatacgat aaaatgtttg
    17221 gctacctgat tgagagtgaa atgaaaaacc tcgacagggt tatgaaagaa gctgcccatc
    17281 cttttactct tatgcttggt ggagataaag ttactactaa aataaatatt gtggatgcgc
    17341 tgttgcccaa gattgataat ttaatacttg gcggaggtat ttgctttact ttttttaagg
    17401 caatgggtat aaacgttggt aattcgcttg ttgaagaggc atatgtggag gtcgcaaaaa
    17461 aaattctgga gcatgcaaag cagcaaaatg taagagtata tttacccgaa gatgtgctta
    17521 ttgcagacgg ccggagtaat gatgccaata ttgagcattg cgagataacc gaaataaaac
    17581 ccaattggat tggacttgat ataggcatta agtctgcaga taattttgca aaagttattg
    17641 aagactctaa aacactgctg tgggatggcc ctatgggagt ttacgaaatg cctcattttt
    17701 cgtatggtac ccaaaaaatt gcgctttccg taggtagaca aaccgacaag ggtttgtact
    17761 cagtggttgg aggcggcgat actattgctg cactgaataa gtacagtttg gctcataaaa
    17821 tcagctacct ttcgtcggca ggaggcgccc ttcttgagta tatcgagggt aaaaaccttc
    17881 ccggcatacg atcgataaag gagtcttttt cggtggatga ttacaatttt aacaggaaaa
    17941 gggttttaat gcgggtcgat tttaatgtgc cactcgataa ggagtttaat attaccgacg
    18001 atacccggat tgttacagcg attccgacca ttcgaaaaat tcttgcagat aatggctctg
    18061 tgattctaat gacccatttg ggcagaccca aaggcggatt tgaggataaa tattctattc
    18121 ggcacattgt tccgtattta tgtaaaaaac ttgaacttga tgtgaaattt cctggagaaa
    18181 gtattggtcc tgaagtgaaa aaaatggcag atgagctgaa accccgcgaa gtgatgattc
    18241 tcgaaaacct gcgttttcat cccgaagagg agaaaaacga tgaaaacttt gcgaaacagt
    18301 tggccgaact gggagatgtg tatattatga atgcttttgg aacagcccac agggcacatg
    18361 catccaccta tatggtggca aagtttttcc cggaagataa aatgctaggt tatttggtgg
    18421 aaagcgaaat taatcatatc gaaagagcct tgcataaggg agaaaaaccc tttaccgcaa
    18481 ttgtaggagg gtcgaaggta tcgacaaaga tcgatattat taaagcttta atggataagg
    18541 tcgataattt gatcattggt ggcggtattg cattcacatt tattaaagct atgggaggaa
    18601 gcataggtaa ttcattggta gaagatgact atattggtgt agccaaagag attctcgaca
    18661 aagccattct tacaaacgtg aagctctatc ttccaaccga ttgcctgatc gccgataaat
    18721 tcgacaatga ggctcacatt cgacatgtga aaattaatga aatcccagat ggctggtcag
    18781 gtcttgatat tggagttaaa tcagctgaaa cattttcgaa agtgattgag cgaagtagaa
    18841 ccatattgtg gaatggcccg atgggcgttt ttgagatgga tcattttagc aatggcacaa
    18901 taaagattgc tttagcatct tcgcgttcca ccgaccatgg agcttactcg ctcgttggtg
    18961 gtggcgatag tattgctgca ttaaacaaat atggccttgc tcataaaatc agttatgttt
    19021 caactgccgg aggtgcactt ttagaatata tcgaaggaaa agagctcccc agcctcaaag
    19081 caataagaac aaaaatttaa agaaataaat tctgtataaa atttttccgt gaaaacataa
    19141 gcggaattct agagaataag tcaagtttca acgagcagtt atggcgtttt tgtgagtttt
    19201 gcctgatatg atattgtgct ctcaataggt ttagctgact ttagtttaga gtaacctatt
    19261 aacaggaaaa taatacttcc caaactacag attatgacaa aaatccaaat atcccaaaac
    19321 gaggcaaatg aaatcaatgg attacgagaa tcataagtta ccataacttc atcacccagt
    19381 tcaaagttcc tgctttcagt aacagcaaca tactggttcc cattcgaggc tgtaaattct
    19441 attgaatatg aaaaactagg gttgtgcccg tgagggccac ttgttgaggt ctgaaaaatg
    19501 tttgtaacaa ctccggtcgc ttgttgggaa tgtttgtaat aatctgcaac ctgaaatatt
    19561 gaaaaaatat ctgcggtaaa aaataataca gcaataataa tcattattaa cccggtactc
    19621 ttactttttt tccccataac aacctccgaa cgtataattc aaatttataa aggaaattga
    19681 attttgatag aggtttctat caaaagttta agaaaaataa tactcaataa aattttaaac
    19741 ctcgcaagcg agaataaaaa aggcaggaat cggatcctgc ctttcaaata ttaatctaat
    19801 attattgtct gatatccaac tcgttaataa tattctgtgc gcctgcaaat ttgtcgataa
    19861 cccaaagcac gtagcgggag tccacattaa tagtacgctg caacgaagtg ctgaatatga
    19921 gattactaca cagccattcc caatttccgt cgaatgcaag tcctataagc tctccttttc
    19981 cgttaataat aggactcccc gaattaccac cggtaatgtc gttatcgctt aggaagcaaa
    20041 caggcaggtt tcctgtttca tcggcatagc gaccatagtc tttggctcgg attaactgct
    20101 cgagtttcgg atcaactaca aattcaggat cgctgttgtc cattttctca ataataccat
    20161 cggcataggt aagccaatga tattttacac catcacgggg atcgtagtct tgcacacttc
    20221 cataagtcaa acgtaatgtg gaattggcat caggataaaa atccctgtcg gtctgataag
    20281 ccctgatggc ttctatataa agcttttcgg ctgaatcaac ttcatccata atgccaatat
    20341 aactcatctg aaccaacatt accccattga aaatggtttc tacgaaatta tacattgggt
    20401 ctttctgcag taactttagg ttgggcttat tcagaaacgc ttccattcgg gttttatcgg
    20461 taaaaatact tctggtatac acgtcggcaa caaatttttc gatagaagca tcttctgagg
    20521 tggctttgta gtttttgaaa atgaagtcgg caaaaacgct attacgttca tcgggtttaa
    20581 tatctttaat gtagagtttt agcattgctt cgaaaacttt tctatcggta gcagggaagt
    20641 attttttaaa attttcttcc atttcgattt ttaactgatc aaccgttaaa tcaatcatct
    20701 cctgattgtt ctttggatct tcgaggtagc tggccaatgc catatagctt tgaacatcca
    20761 tagccctgct gcttccctgc aataaaccaa atgcaaggta gtacattgct ttggttgcct
    20821 ccccgtaggt tttgtagttg gctgtcaggt tttcgaaagc ttcgccatac ttacctttgc
    20881 gtttttcgtc tgcgttaacc cactgcataa aaccttcttc aaatgaacgc ctttgtgcta
    20941 tagcatcggt ggtttttagt gtttgcattt ctccgtcgaa aagcttacgg ccgttagcat
    21001 aatttgcata cacatctgcc atggcaagct ttatttcagg attattgttc atgtcaactt
    21061 tcataatatc gagcattgta cccagtaatg tcaccaaaat cggatcataa taatcgcgtt
    21121 tatattccat accggctgcg gaaagatatc tttcggtttc tccgggatat cccattatca
    21181 tggtgaaatc gccctcttta actcctttga tcgatattgg aagataatgt atgggagcat
    21241 agggaacatt gttcttgtcg taaccttcgg ttggctttcc atccggcgac atatatactc
    21301 tgaaaataga aaagtcgccc gtgtggcggg gccacatcca gttgtcagtg tcaccgccaa
    21361 acttgccaat cgaagacggg ggggcaccaa cgaaacgtac atcgccaaaa acttcataaa
    21421 cgaaaagata atattctccg cctttataca tttctttcac attggcatta tagtggttgc
    21481 cttcgatagc ttctgtttca attttatcga tagctttggc aatcattttt tcacgatctt
    21541 cagcaggggt atcctcagta ataccggcaa aaaccttgtc ggttacatgt tcaattcgca
    21601 ccacccgaga aaccgaaact ccggggacgg atatctcctc atcgaaattg cgggcccagt
    21661 atccgttgtc gagatagttg ttttcgaggc tgcttagtgt tgcaatagcc tcgtaaccgc
    21721 aatggtgatt tgtgaagaga aggcctttgg aagatacaat ttctcctgta cagaacccat
    21781 aaccaccttc acccagtaat tgaaaaatag catctttcac agaactgttg ttgatgctgt
    21841 atatctgttc gggtgtaagc tcacagccca gtttttgcat atcagcataa ttcattttac
    21901 tcatatagcc gggtatccac attccttcat cgggatacgc aaaaagactg cccgaaagta
    21961 ggagaatgaa aaagaaaaac attagttttt tcatagactt tgtattattt gtttatgata
    22021 caaaaaaagc ataattattt gataactggg attttttatc ttaaaatgtt cttaaagcca
    22081 aaataaaaaa aactgccata ttgtagaata tggcagtttt aatagagata atgtcaaaat
    22141 ttagttgagg gttaaactgg tatgggcatt acctttcgaa gttttaacaa cgatcatgta
    22201 attgcctttc tgtaaatgac ttagatcaag aacagcttta tcagcattgg gctctgaacg
    22261 aaatacaatc tgcccgattg tattgtaaac ctctacagcg cttataccaa gcccttcaac
    22321 atgaataatc ccatttgccg ggttggggta tacggtgcat attttagtga aattcggatt
    22381 tacaaataca ttggttgtaa ttgtaatata attttctgca atgaaagttg aggtttttgg
    22441 cggagtacag tttccattag taacagtcag ggttacatca tatacaccca ttgaattcca
    22501 tgttgctgtt ggattttgtt cggtgctgcc gggttcgttt gctccttcaa aggtccattc
    22561 ccatgaagtt gcgttaaaac tggcatctgt aaaagcaatc ggaactccac cttctgaagt
    22621 ggtcggattt gcagtgaatt cagccacagg tggtgcctga ttctgcggac ggataacaac
    22681 ttttgcgtcg tatcccagtg tttcttgtgt aacaggaacg tcaacaggaa cagaaccata
    22741 aagcaatact gttacagttg cagaaaactg aaatggaaac tcgccctctg ttgttggggt
    22801 gccaaaaata attccacaat aacgcgtaat cgggtttgtt ggaataaatt gttctgcgtt
    22861 ggtgtcccag tcaagaccat caggaagacc tgttacgctg ttgattacga ttttctgaac
    22921 agtcagatcg tagccattgt aattgtaggt ttcaggtggg atgaacgtaa ttacctgtgt
    22981 gtagggaacg tcaacatatc ctgtgtcaaa taccattggg cagatctgac ccgggtcgag
    23041 aacatcttcg catccttcgt cgggagtaca ttgcgacatt gcattaaagg ctgcaaaaaa
    23101 gccaataaca aacagtagag atagtttttt cataatttta aaatttagtt tcacgtcaaa
    23161 aataaacctt tttatatata aattgaaatt tgggtcgctt tgttggtata atttcacatt
    23221 agaatttagc ggtaagcatc agttcatacc ttctcatagg ccttaaatca ccgggtctgc
    23281 caacatattc cgaattgaac agattcttta cgagtagtga tatacggatg atttcactaa
    23341 atttgaatcc caatctggca tcaaaagaaa catacggttg gttggcatga tcaatcctgt
    23401 aatcccaata tccgggaagc agcgcagcat tgctcataaa atagtaaaac tgatataagt
    23461 tatactgatc tgttgttaag gttgaaggat ctctttcatc acagaacaag cggtcgacat
    23521 ttttcatagg gctgttgaga atccagttaa atccaaacgt aaggcgtttt gcttcgaagt
    23581 cgaaatctcc tttgaatgaa tgaacatatc tatatttaag gattggattg tcagttgatg
    23641 cagtagagtc ggcgccggct atgttaacgg ggtaattata tgcatatcca agcataactg
    23701 tagttttaag cgggcccaat ttcccgctgg taacaatgga taattcacta cctagaattc
    23761 tggcatctgc catattccgt gcctggaagc caaaatcacc ggattcgtac agcgatacag
    23821 gaagcagggt aacaggatcg tacttgccaa tggtaaattc aatcatatta taaatttcct
    23881 gcatgaagaa cgacaaatcg acaaagcctt tccagccttc ccaaagttta attccttgtt
    23941 ttacaccaat ttctgcactc catccgtttt cgggttgtag gtatggattg ggaagaattt
    24001 ttaccatcga gcctaaatca gatgcagtgt atttttccga tatagtcggg aatctatagc
    24061 cctgtcccca cgatgctcgt aaaaatgtat attcaaaaag ctgatagtta agacctgctc
    24121 tgaacaccgg tttaaaaggt atttcaaagt tcttgaaccg aactaacgat tctgtttggg
    24181 cggtatcaac tttgtaatat tccccgcgta acccaaccga ggctttcagt ctgccaaact
    24241 tccagtcgag ctgcccatac aatgcaaagt tgttatggaa atgatcacca aataagttgg
    24301 aatgcacctc tgaatagcca ttaacaccgc cggcagttac gttccccttt tcaccaaggt
    24361 tggtactata ggtatattca atataatata agtccccgtt actgtttttg gctgtatcgt
    24421 tcggaatata ctgtcgggta ccgaaatatc gtgttctgat tttatatttc gacccattgg
    24481 gtttgtaata ataaataaac gggtcaatat ttacacgata tgcttgtgtg ggggcaaaca
    24541 gtttatcgac ttcgggagta tttagtggca gtgtatcgga atgccagaaa aagaaatccg
    24601 acttatcgac agccataaaa tttgtattca tgccaaaagc aagaccttcg atttttttac
    24661 ttctataacg ccagtttaca ttagccctgc tgcggatttc gtactccttc tggcgatatc
    24721 cctcgtcaac aaagtggcta ccacccatgg taatgtcgag gtttccaaat tgttgtgagt
    24781 gaaacaggtt taatccggaa tacatcgggt ttttaatcca ataatacaac ccgttccgta
    24841 aaggaatatg ctggtaaaat aaagagtcgt taaaataaaa atcttcgtcc caccatttgt
    24901 aactttcagt tttcgggcta tcgtaaacac caataaaata gcttacccta gtttcaggtt
    24961 tgtcagtcgg ataagcagat cttacgttaa ttacaccact caatgcggaa gatccaaaca
    25021 aggctgatga tgctcctttt acgatttcaa tctgagaaat gttttctaca ggcaggtaat
    25081 tccatttgat gtcgcccacg tcagccgaaa ctattggcaa gtcgtccatt agcataatta
    25141 cccttgttcc ggcaccgtag ctccaccccg aactgcttcg gatactcaat tgctggtcgg
    25201 aaacatccac acccgtaatc tgatttacag aggtttcaag actaattgtg tttgtgtttt
    25261 gaatcatggt aggtttgatt acctccatgg taacagtaac atccgaaagt ttttgctcga
    25321 atttacccgc actcaccact acttcatcga tgagttctgc acttggtttc aggcttaagt
    25381 cgagtgtttt tacttcgcct gctttaagag taatcttcat tctctcccgg tgaaacccga
    25441 tatactgata tgtaattgta taagttcccg cagagagatc taaagtgtat ttgccatcga
    25501 aatcggtagt agtaccggtt ttaacgttaa cagtgtcgtt agctcctgtc acaatcacat
    25561 ttacaccata aagaaattcg ttatttgaat tgtcttttat ggtaccagtg atttttgccg
    25621 tttgcgagag gaggagggtt gtggagaata aaatgaatag agttgtcgat aataacgatt
    25681 ttatcataaa catttcagtt ggttaatgtg ggcaaatata tattttattt tgatatctga
    25741 taccttgtaa ggtcttgccg aaaagtgaac atatcaacaa ccggtgttca aaataaaagg
    25801 cgcttgcaat taacaaacgc cttctatgat caaaggatta atttcgttac cgcagcaatg
    25861 atagttggcc gctttgagtg tagatattcc caaaaggatc ttcaaacgaa attatccagg
    25921 tgtaaatacc ttcggggcac tctttgtcgt gcgacatacc atcccagcca acctcgtaat
    25981 cgtttgtaac aaatattcgt tcaccccagc ggttgtatat ctccatttgg aaagtttgtc
    26041 tgtcgatacc gtctccaata attctgaatg tttcatttat caggtcatgg tttggagtaa
    26101 aggctgtggg tgcataaatc attgtttcgt tggtaactgt gatgtcaaca cccgttgaat
    26161 caacgcatcc atatagtgat tcagtagata aggtaacatg atatactccc gggaacgaaa
    26221 attcatgttg tggattggta acggtggttg aggtgccgtc gccaaaatcc cagttaaaga
    26281 agaagcctcc ttcggtaaag ttggtgaacg atacagcagg acttgccatg tgtacgctgg
    26341 ttgaagaaga tacaaattca gcttcaggaa cggggaaaac aactatcccg attgttgaat
    26401 cacgtttgca tccttcagca cttgttattt caaggcgaac atgataggtt gtggcattaa
    26461 agaaagtgtg tacaggatat ttatcgaacg aaaggttctc aaaatcacca tcatcaaaat
    26521 tccaaatata ccgttgccca atgtcttcgc tgttctcgcg gaaagaaacc gtaagcggag
    26581 cgcagccatt gtatttgtcg gctgaaattg aaagcggtgg taaaggatac gtggttattt
    26641 ctttctcaat ccgaacaaac tgataattgc aagaatcgaa aattacaaac tcaaaagtag
    26701 tgtcgttgaa cacctgcaac gatatcggca aatagtacat cgagtcgttt atcataacat
    26761 aatttcccga aacaccgcca ccgcttatgt taacatcgaa atcaacaaat tctccggggc
    26821 acacatgttc tgattctgca tcaacggtca ttgcaatagg atcgtacaca tgaacggtga
    26881 taggcaatgt gttgctgacg cagttatttg aatcgacaac cataactgtg aatgtgctat
    26941 cggatagtgg gtggacattg gtagtcatac cggttgcacc attgctccac agtactacat
    27001 agggaggggt atttcctgta acaagattca atcccaatgt tgcaatcata ccatagcaaa
    27061 tctgtgggtc cggataagtt tcaatcacat agggttcggg ttcagtaact tcatacgaca
    27121 taatataatg gcaatacctg tcgtctgtaa ctgttaccct gtaaatgccg gcttcaacat
    27181 taaactggtc ttcttcccag ctgccgttcg accatgcata atggtatgga ggagtacctc
    27241 cggttacatt gatgtacaca tttccgtcgg aaccaccatt gcatcttaca ttggtaccgg
    27301 taattgttcc agccatggga gcttcagact gataaatctg aacctgagcg taagcaacgc
    27361 aatcgttttc atcaaacaca gtaacactat acattccggg ctctaagtcc agaatatctt
    27421 cgctgatttc gccgttactc caggcataat aatatggagg tgttcccccg aatacggtga
    27481 gatcggcaat accgttttct tctccgaaac atcttacgtc agttggatca atatatgccg
    27541 ttaacggaag gtcgggttga tcgatacgac caatggcata cgcttcacaa tcgttagcat
    27601 cggtaactgt aatagagtag attcctgcat attgattgaa taaatcctga accgtagctc
    27661 cattgctcca aaggtaggtg taaggtggag ttcctccggt aacaactgca ttaacccaac
    27721 ctgtattctc gccgtagcag agtacatcct gaataataag ttcaacttca agaggtggag
    27781 gcataataat tacaaaagtg tgttcaatat cgcagccatg agaatcaaca agggttattg
    27841 tataagcccc gtcggcaaga ttgtacaggt cttcggtcat ctgattattt gaccagtagt
    27901 aatagtaagg tggtgttcct ccggttactg taaggtcaat aaagccgttg gtatatccat
    27961 aacaggtcac gttaccatat tcccatgtca tttcgagggg ttcatcgggt tcgtcgatga
    28021 ataccgaatc gagatgtgta cagcctacgg aatctgttac tgtaagataa tgccaaccgg
    28081 cataaacacc aacagggttg gggtcggtgg aaccagatgg ttcccataaa tatgtgtatg
    28141 gaggtgcacc tccggtaaca tatgaataca ggtttccggt tggttcgccg gcacaaagca
    28201 catcatcggc atgcaaatag gttacaatgg gtggaaaagc atcggcgatg tttatttcac
    28261 ccgaaacgga gcacccgtaa acatcggtaa cggtaacaaa ataatatcct gaaggaacta
    28321 tcagggaatc aatcggactc atatgggtat gttctgatcc ccatgaatag gtaaaggtaa
    28381 agggaccgga actgaaaatt gcagtgacag taattacacc cgaagtatcg ccgaagcaga
    28441 gaacatcctg tgcattaata tccatgtcaa gttcaggtaa gggatacagc caggttcccg
    28501 ggcatacggt gtcaagaagg aagccttcca tttcccaggt ttgatcatac caggtaagcg
    28561 ggatataccc caagggaaaa ctttgctcga aaagcgggcc gatttcaaat tcaatatctt
    28621 tcaaaggcat tgaatcggac ttgatctcag gttccggaat aacattactg cttttttgtg
    28681 aggttaggtc aatttgaggc ggagaaataa tattgatttc gggtgatgca taccgtagag
    28741 tttgagaata accaacatct ttatattcct gaactattgg gggtaattca aattcgggca
    28801 tttctgccga ctttatcgga aaatcgaaag gtatggttac gtgagcataa aataccgaaa
    28861 caataaagtc gaatgcaata ctgtcccatg tgtggttatt gaaatcatta tcgaagtgat
    28921 atgctacggt tacaggcatt gaatcccagt cgtaacacgg atatgtgatg ttaaggtcat
    28981 tgtttacttc aaaagtaatg tcctgtgcgg ttccctgatc gaccaccgta ttgccgttgt
    29041 cggggtcgac caccgaatag acaagcggtg ttgggaaccc gagcgtaacc catatggttg
    29101 ggcaaaatga aaattcctgc gtcatataac tagacatatg caaatcgaga tgaagcaaat
    29161 agtagtcgat aattgcattc actcctggca taatttcgta ggttattgtg ccaccgttta
    29221 tgattccgat aatttcctga attgcaggcc cctcaggagg tggaataaaa ccggctatcc
    29281 atgaaagaaa agtaagaata ttcaggttga accaaatcca ctcatcttgt ccgtaagcgg
    29341 tcaagcactg ggtgtcgggg tcaaggtaat cttcggtttc aacgtaaggc aggtcaattt
    29401 caccggtaag cccaattcca aaccagtcgg gaattacaat tggcaggatg tcgtcttcgc
    29461 aaagcatggg cagtcctgta atttcgtcga tgcaaggata aaccacttct ccggtgtatc
    29521 cgttcaggta gaaaattgct atagaatcgt gaggtagcgg atcggtataa tccgggtcaa
    29581 tggggatgct taatgtaggc aaaataggaa tggtctcgca gtcgaacata catactatta
    29641 tgttcatata tacaccaaac ccatattcaa ggtcgagggc aataatacca accgttggga
    29701 agtgtgtgtc gagataccat ccgtcttcca cttcatacga ggtaaatacg gtaacggttt
    29761 caccgtggtc gaagctgtaa tggtcgggaa aatccagggt tatttctacg ggatagttca
    29821 cgtcaattga tcccaaggta aatccgtgta tgctaaatgt tgaactcagg taagcccaaa
    29881 atccggcttc ccatgcaatc ccaactggcc caagtaaagg aacgtcttca atgaaatcta
    29941 gagtagggtt ttcgctgaat tcaaggtgaa atagttcata atcgaaatca agattaaatg
    30001 ccccaccggc tccccacata tcctggtcgt aagaataata gtgaatattg tgaattagaa
    30061 tattcgaatt tgtttgtgct tttactcctg aaatccccgc aaagatcaga aatattacca
    30121 gagctatttg tattttttta ctcatattat ttaataaatt gagctgttaa aagactattg
    30181 ttgaaaatat caaaaagtaa gtttcgagaa acggcaggat agcttacatt aatccaaatc
    30241 gtattattat agtcgcctgc aagaaacatc attctttcgt aatcaacgcc tttattggta
    30301 aatcccagaa tgaaaacaca accatcgtgg ccatcgttgg tggtaatgtc acgcttttcg
    30361 atatacctga aattctgact ttcgacgtat tctggggtta ggcttttaga aaccataata
    30421 taacttgttc cgataatttc ggatatctgc atggtggaag atgttgcacc atgaacaaat
    30481 ccctgaagct cagctgagta cataaaatgc tcaggtggta ttattttgac atgtgtgttt
    30541 gggattaaca cactctcacc cggtgtagtt atatatgcat caccggttcc ttgcaattca
    30601 ccatttgttg tcaggttcaa cgggacctgt gcaaatgata ctactacaac aaaaaaacct
    30661 acaatcgtgt gtattaccct taacatagta ttttcagact tcaaatataa tataattaat
    30721 tcaaattata gtaattcatt gacgaaaact tcgttggaaa gttgcttgta agatataagc
    30781 atttacaaat ttgaaaatat tttgtgcctt cgcttatgtg tatgtaagtt tttgtatttt
    30841 tgacctccaa aaaaattaat attatgaaga gaactctttt gttattgccg gtattatttg
    30901 cagtagttat tagttgtggc tcaggtaaga acaataaatc ggctgcaggt aaaaatgata
    30961 accctgttaa atcgggcgat acaattgtaa tcgattatcc aaaagataca gtttttgatc
    31021 agattgcttc gttgctttcg ggagaaaatg cctcaagttt taatgatacg tttgcgcaat
    31081 cacaatactg gaaagattat aaaattagta ttgatactcc atggcagcgc atcgaaaatg
    31141 aaagactctc cataatttac aattggtctg attcagttat caaacctctt attaacgata
    31201 ccagcctgtt gttttatcca ttttcgggcc ccgattttgt tcattcttac tatctgtttc
    31261 ccaatgccaa tgaatatgtg tttatagcca tggaaaagct gggaacaatc cccgcccttc
    31321 ataaaagttc ttttgaacag ctgaaagcat atctcgatgc cacaaatttt gctatcagag
    31381 acatctacaa acgcagttac tttattactg gaaacatgga acaagatctg agaaagcagc
    31441 aggtcgacgg ggtactgccc gtattactcg tatttcttca taaaaccggc cacgaaattt
    31501 acgattttgg gtattatcga ttaaacaacg atgctaaaac tttcaccaaa atcgacaaac
    31561 ctaccaatag cctcgatcag gtagaatgta tccgtttttt aattctgaaa aaaggggata
    31621 ataaactcaa aaccttcacc tatttttacg ccgatatttc tgacagtgga tttgccaaaa
    31681 caaaaacact gaaaaaatac ctcgataaca tgcggtcgtg taatacattt attaaatcgg
    31741 cttcgtacct gagccattat ggcacatttt ctaatattcg ggatctcaca ctaactaaaa
    31801 gcagtgccgt actcgaagat gatacgggag ttcccttcag atattttaca tcagataagt
    31861 ggaattataa gttgtttggt gtttatgtga agcctattgc tgatttcccc agctcatacc
    31921 tgtatcagaa agaccttgct gctgcttacg actctgttgg ggctcaggca ttacctttct
    31981 cccttgggta ccactggaga acgggagagc aaaactggtt gctgttttac cgaaaataac
    32041 agtttcatac aaacagctca gaaataatac tgtccgaaat caaccagtat ggttccggta
    32101 tgataattat tccacgattg ttctttacag aacctatctg aataaatttc tttgagtttt
    32161 ccaataagcc ggcgacttcg ctctttccaa acaaattctc aaattcatgc aaattcaagc
    32221 catcggaggt tcttaaccga gtcatgatgt gttcatttat tttatcggaa ttctcgaggt
    32281 tttcgcaaac tgtcacagac tgatttttag gtattgaata aagatacttg tttaggtcgg
    32341 ggggattata ataccttttg tttccgtcgt atgaatgtgc cgaaggcccc aggcctagat
    32401 atatactatt gtcccaatag gagctgttgt gttggcttcg gtatccaggt ttgcagaaat
    32461 tagaaatctc gtaatgatcg tatccgtacg tccttagggt gtcgcataat aaactatatt
    32521 gccggttaca gatttcttcg tcaggaagcc tggttttccc ctccctcaat cggcgtacca
    32581 acaatgtatt cccttctgca gtaagcaaat acgctgaaat atgggtaaga ccggcctcag
    32641 tcagaatagc aatgctctca gccagttcgt tctggcttag gtcgggtatg ccgaaaataa
    32701 tatctgctga aatgttgctg aatccggcat ttcgggtatt ttttaacgat tgaagtgcaa
    32761 tctcggaatt gtgccgccgt cctagaaaaa gcaaatgcct gttgtgggtc gattgtatgc
    32821 caatgctgat gcgattaacc cccgaacttc gcagggtttc cagatattca ggtatggcat
    32881 cttctgggtt tagttccacg gtgaactcct gtaaccagcg caaatcgtaa atttctccca
    32941 attggtttac aaatgagctg atttgctctg cagtaagcat tccagggctt ccaccaccga
    33001 tgtaaagggt gctcacaggt gtagagctaa aaaatcgctt attccgggct gcttccagta
    33061 aaataccgtt gagtaaatca tcggtttttc cggtttgcgc cacggaataa aagtcgcaat
    33121 agacacattt gcgcctgcaa aatggtatat ggatgtagat gccgggcaat ttcaaagtat
    33181 tttgggcaaa gttattaaaa gcgaaagaaa aaaataaatg acgaatgggt tatataattt
    33241 tgctaacctg aaacaataca ctatttttag caaataaata tcaaaaataa tgaaaatcat
    33301 cacttttaac ctgaatggga tacgttcggc gcaggcaaag ggtctgatga actggcttaa
    33361 aatcgaaaat cctgatgtgt tgtgcgtgca agaaaccaaa gcgcaacccg aacagatcga
    33421 tttccacgaa tttcaagaaa tgggatattc tgtgtatttt cattcggcac aaaaacccgg
    33481 atacagcggc gttgccctgt tttcgagagt agctttcgat aacgtaacgt acggaattgg
    33541 tgtaaaggaa tttgattcgg agggacgttt tattcgtgct gacttcaata atacagtatt
    33601 gattaattcg tatttcccct ccggaactat gggcgacgag cgtcaggaag tgaaaatgaa
    33661 atatcttgat gcattttata actacaccga aatgctgaaa aaagttcagc ccaacctgat
    33721 tgtttgcggt gattttaata tttgtcataa agagattgat atcagtaaac ctgaaaacaa
    33781 aaaaggggta agtggttttt tgccggaaga aagggcttgg gtaagcaagt tcatcgattt
    33841 gggatttgtg gatgttttca gggaattcga caaaagtcct ggtaaatata catggtggag
    33901 ttatcgcgcc ggtgcccgaa agaaaaacct tggctggcgc atcgactacc atatggtaac
    33961 cgttccattg aaaagcaagc tttccgctag tagtatactg agcgatatcg cgatgtccga
    34021 tcactgcccg gtggtcatgg aaatagcact ctgaaagata ttatttgaat gaaaattttt
    34081 ctacttttgc atcttgatat cactcaacta tgagctcaac aaattatttt gcgcacgaaa
    34141 ccgcaattat cgatgaaggt tgtcagatag gtcatggaac aaaaatttgg catttttcac
    34201 acatcatgag taattgcaaa attggagaga attgcaatat tggccaaaac gtggttgttt
    34261 cacccgaagt tgttcttggt aataatgtga aggttcagaa caatgtatca atctataccg
    34321 gagtagtttg cgaagatgat gtgtttttag gcccctccat ggtttttaca aatgttacca
    34381 atcctcgaag cgccgtaaac cgcagggggc agtatgccaa aaccctggta aagactggtg
    34441 cgagcattgg agccaatgct actattgttt gcggacatga tattggtcgt tttgcattta
    34501 tcggagctgg tgcagtcgtt accaaacatg ttcccgatta tgcgctggta atagggaacc
    34561 ctgctcggca cgccggatgg atgagtgaat atggtcataa gttgcagttc gatgaaaatg
    34621 gaatagccat ttgtccggaa agcaaggaga aatacgtatt gaaagagggt attgttgaaa
    34681 aaatatcaat gtaatggaac catcagttaa aaattttgca ttggtcggcg tagccggcta
    34741 tattgcgcca cggcacgtga aagcgataaa ggaaacaggg aacaagcttg tggcatgtct
    34801 cgataaattc gattgtgttg gatacctcga ttcatttttt ccggaggcag atttctttta
    34861 tgaatttgaa cggtttgaac gacacatcga taagcttaaa tacaccgacc gcaaaataga
    34921 ctatgtaagc atttgttcgc caaattatct ccacgactct cacatcaggt ttgctctttg
    34981 gaacagagcc catgccatat gcgaaaaacc actggtgctg aacccatgga atatcgaagc
    35041 gcttatgaag ttagaacagg agacaaattg caatatttat aatattctac aattgcgctt
    35101 gcatccagca attattgcac tgaagagtga aattgaaaat ggaccgaaag ataaaattta
    35161 tgatatcgat ctcgcttaca ttacaagcag agggaaatgg tatgactaca gctggaaagg
    35221 gaactctgag aaatcgggtg gagttgcaac caacattggt gtacatttct tcgatatgct
    35281 cttatggata ttcggaggaa tcgaaaagag cagggtacat ataagcgaaa cccacaaagc
    35341 tgctggtttt ctacaattga aaaatgccag agtacgctgg tttttaagca tcgactacaa
    35401 tgatattcct gaggcaatac ggagcaaagg acaacgaacc taccgttcaa ttacagtggg
    35461 taacagggaa atcgaattca gtgaaggatt taccgattta cataccctga gctataggaa
    35521 catactggaa ggtaatggat ttagagtggc tgaagccatg ccatgtatcg atgcggcata
    35581 tcaaatccgg aatgcaaaac ccataggttt ggttgatgaa tatcacccgt tatgtgctgt
    35641 aaaaaatcat attttttaat gtgaaataat attacatttg tcgcgcttaa cacctcaata
    35701 ataatttatt aaaaccattt tttatgtatc agagcataat taataaacaa aagaaaattt
    35761 ccttaattgg tttgggctat gttggtctac cgattgcact agaatttgca aagcatgttc
    35821 ccgtagtggg cttcgatatt aaacccgaga gaattgattt gatgaaaaag ggaatcgacc
    35881 cgagtaacga actcccaaaa gaagctttcg caggctgcga tattatgttt accgcaaacc
    35941 cagaagattt gagagattgc catttccaca tcatagctgt tccaacgccg attgacgacc
    36001 ataatcttcc cgatttgacc ccggtaataa aagccagcga aacggttggg agaattctta
    36061 aaaagggcga ttatgttgtg tatgaatcca cggtttatcc gggttgcacc gaagaagatt
    36121 gtgtaccggt acttgaaaaa atgagtggat tgaaatttgt tgaagatttt aaagttgggt
    36181 attctcctga acgcatcaat cccggtgata aagaacatac tctgacctcg atcaaaaaag
    36241 tggtttcagg ttgcgatact gaatctgccg aaaatatcgc gaaagtttat gaaatagtca
    36301 ttaaagcagg tgttcaccgg gcttcgagta taaaagttgc cgaatcggca aaaatcatcg
    36361 aaaacacaca gcgcgatgtg aacattgctt taatgaatga actgtcgatg atatttaaca
    36421 ttatgggtat aaatacctac gaagtgctgg aagctgccgg aacaaaatgg aatttcctca
    36481 aatttgttcc aggtttagtt ggcggacatt gtataggagt tgacccttat tacctgttgc
    36541 ataaagcaaa agaacttggt taccatgcac gcatgatcaa cagtggcagg gcaatcaacg
    36601 atggcatggg agcatatgtg gctgaacaaa ccgtgaagaa aattattgcc atgggaaaag
    36661 agattacaaa ctccagagtt ttggtcatgg gcgtaacttt taaagaagat gtaaccgata
    36721 tccgaaattc aagagttgtt gatattctca acgaactcag taaatacaac gtaaaatacg
    36781 aagttgttga ttgtcacgcc aatcatcatg aggttgaaga agaatataat cttaagatgg
    36841 tggaaaaacc cgaaggtaaa tacgacgctg tgattgttgc tgtaaatcac aaggaatata
    36901 aggatctcga cgaaaactac ttcagcagca ttctgaaaga tcgaggactg ataatggata
    36961 taaaaggtat gtacagggga aaaattaaaa atattgccca ctggaccctc tagataaaca
    37021 aaattgcaat tctaagggct gactcaacaa tcagcccttt ttttagttta atcgtgtaca
    37081 atttttttgg tattcaacaa taaacctgta cttttaatcg ataaaaaatt aaattagttt
    37141 agatgaaaag accggttgtt ctggttacgg gaggtttggg gtatattggg tctcacacag
    37201 ttgttgagct tcagcagtca ggctttgatg tgattatttt cgataacctt tccaattcct
    37261 ttattgaagt gttggatgga attgaaaaaa ttacgtcggt gaggccggga ttttttaaag
    37321 gagatgtcac gaataaaaaa gaccttgagg cattattcga cacctatgat gctattgaat
    37381 ctgtcattca tttcgctgct ttaaaagcag ttggtgagtc ggtggaaaat ccactgaagt
    37441 attatcacaa caatgtgtac ggaatgatga acctgctgca ggcatcaata aagcacaggg
    37501 tgaagaactt tgttttttct tcgagttgta cggtgtatgg gcaaccacgg atattacccg
    37561 tcgacgaaac ggctcccata caaccagaat ctccatatgg aaatacaaag tggatttgcg
    37621 aagaaatgct tcggcaggta atttcatcag gggtggcaat gaaagccata tcactcagat
    37681 acttcaaccc catcggagct catccgtctg ccgaaatcgg cgaactgccc cagggggtac
    37741 ccaacaattt gatgccgtac cttacccaga cggcactcgg tatcagaaag gagctcgtgg
    37801 ttttcggaaa caattacaat actgcagatg gtacttgtat acgcgattat atcgacatca
    37861 acgatttgtc gaatgctcac gtggttgcac ttgcgttcct ttcaaatggt tctgcaaagc
    37921 cgtatgatgt tattaacctg ggaaccgggc tcggtgtttc tgttctcgaa atgatcagag
    37981 cattcgaaga aagttgccac gtcagcatta agtttcgtat cggagataga cggggcggag
    38041 acgtggaaaa agtttgggca aatgccgata aggctttgca ggtattgggt tggaaaacgc
    38101 gtatttcgct tcatgaaact atgaaaaatg catggtattg ggaaagacga ttccggaatg
    38161 gtgaaaccgc aataaaaccc ttataaatca gtttctgttt catccgatga attacttaat
    38221 tatcttatta tttttgccac aatataaata gtgtagaaaa gaatatgaag aaaaccatca
    38281 ttattaccgg aggagccgga ttcataggtt cacatgttgt gcgattgttc gtcacaaaat
    38341 atcccgatta tcgtattgta aacctcgata aactgactta cgcgggcaat ctggccaacc
    38401 ttaaggacat cgaaaacttg ccaaactatc gctttgagcg tgccgatatt gtggatggtg
    38461 atgatatgat aaagatattt gaaaaatatc aacccagcgg tgtaattcat ttagccgctg
    38521 agagtcacgt tgaccgcagt atttcgaatc ccacggaatt tatttttacc aatattgtgg
    38581 gcacggttaa tttgctgaat gctgcccgac atatctggca gggaaacttt gaaggccgtc
    38641 gtttttatca catttcgacc gacgaggtgt atggctcgct gggtgatgaa ggttttttta
    38701 ctgaagaaac agcctacgat cctcgctctc cctattcagc atcaaaagcc agcagcgatc
    38761 acctggtgcg tgcctatcat catacttttg gtttgcctgt ggtggtttcg aattgttcaa
    38821 ataattatgg tcccaatcat tttcccgaaa agttaatccc cctggccatc aataacatca
    38881 aaaacatgaa acccattccc gtgtatggta agggcgaaaa tgtgcgcgac tggctttatg
    38941 tgctcgacca tgcccgtgct attgatacca ttttccatca ggggaaaaac ggggaaacct
    39001 ataatattgg cggtaacaac gagtggaaaa atattgacct tatcaggttg ctgtgccaaa
    39061 taatggacaa aaaattgaat cgcaccccgg gcaccggcga atcgttgatt actttcgtga
    39121 aagaccgtgc cggacacgac ctgcgctatg ccatcgattc caccaaactg cagcgcgaac
    39181 ttggatggaa gccttctctt cagtttgaag aaggtttgga acgcaccgtt gactggtatc
    39241 ttgcaaacga agagtggctc agcaacattg tgtcgggcga ctacatgcaa tactacgtgc
    39301 aacagtacgt taaacgataa attaatgacc gaagaaatat tcagtacctt accatttgcg
    39361 gttacggcat gtaataccga ggggattatt gtttacatga acgaaaaatc gaaagcaaca
    39421 ttcgagaaag acagtaatgt ttcgttgctt ggcaaatcgc tgttcgactg ccacagcgaa
    39481 gcttcatcgg caaaaataaa ggagctgttg caaacaggtg gcaccaatgc atacaccatc
    39541 gaaaagaatg gtcagaaaaa gctgatttac caatgcccgt ggtttaagga ggggatggtg
    39601 gccggtttgg ttgaaatctc gctaccgata ccacttgaga tgccgcattt tgtaagggga
    39661 taaaacgaaa taaaatatat tacgtttatt gatacttctt tgtgcaaagt tgatatttgt
    39721 tttgaagcag gaacaataaa tcaatttccc gggttaaaac cactatacaa ggattactca
    39781 tactgtcacg acactttttc tgtttttgaa ccaggggggt catatgtctt cttttattct
    39841 gaggatagtt taattatgat ttggtatgga gtttatgggc cacctgatta tacgcattat
    39901 cttgagaagt ttattggaat ccgcatccca aatacttctt atgtttggat taaaagctat
    39961 actcccaaag gaaatatttc tgtttaccct aacccagcta ccgatatact gtacataaac
    40021 atgcccgaag acatgggaga aacagaaatt gaaatagtcg attttaccgg aaaaactata
    40081 tttaaaactc gtgtttggtc atcggattac aacatcaata cttccttgtt caaatctgga
    40141 ttttatgtga ttatttgcaa aaaggatggc attttatata aaactccctt tttagtaagc
    40201 aggtaatttt tttgaataaa gagtttgaat gtatagacta atttctggca gatctaatat
    40261 tatgcgtaaa gcttaacaat tcgttcaatt actttatcaa tcggatattt ttcgtgatcg
    40321 aaaatatact ggataaatgc tccctgcaaa actgaaatac aataggtttt ttcaaactcc
    40381 ggatcttcgt aacccaacgc aataaaatac tcgcgaagca atgtatcgat atcgtcggct
    40441 cccttgagtt taaaatcgcc aagtgatgtt aacgtgccgg gttgcagtat taaattaacc
    40501 agtagttgcc agaagttatg attgccatca atcaatgatt taattcgata cacaaaatca
    40561 acaaactctt ctttttgtaa aatgccatcc tggttctggt cgaaattttc gtatgcctga
    40621 ttaaaacctt cctgaaaaat ttcacccagc agtgcttctt tgctgtcgaa ataattgtac
    40681 atcagtccct tcgacatacc tgcttcacga gcaatctgac tgatagaagt ttgatggtat
    40741 cctttttctg caaaaagacg caatgctgta tccatgatca atccccgtct ttctcgcctg
    40801 atttcttcga attgtatttt gcttttagga cacatgtttt ttattgaatg aacagtcggt
    40861 caaaaataaa gaaaaaattc attatgacat aaattgattg ataaaaatgc attaatttgt
    40921 gtaaaattct ttcaaatgaa aaaatcaagc tactttctct tgctttcaat atttatttta
    40981 agctttttct cagatgtttg cgcccaagct ccggttgttg ttccggcaga acaccccgga
    41041 atggttggcc tttggacctt cgacaaccaa tcatcgctaa tgcaggcaac aactggaagc
    41101 gaccttgaaa cacatggtac tgtaaatgca atatctggcc ctgttgccgg tgataatgca
    41161 ataaatattg gtgttggtag ctatctgacc tgttaccata atattgcagg taacggaggt
    41221 ggatcagaag taaatgaata tacgatggtg tacgatttta aaatacctgc tactggcgtt
    41281 tggtattgtt tttatcaagc caacgagagt aatagcaacg atggtgaatt gttcatcaat
    41341 ccttcgggca gtataggtcg atctaccgac gggcccggtt attcagtcta tcaggtaagc
    41401 cccggagaat ggtaccgtat ggttgtttca gtagatctgg gcaattatta caaagtttat
    41461 cttgatggcg cccttattct tgcgggtgga agcttgggtg ttgacgggtc atattcgttg
    41521 tatcctgccg acgatgaaaa caatgttctt tttttcgcag ataacgacgg tgaagacaac
    41581 agttttgata ttgctctaac ggctatttat aattattcgg taaatcaggc cgaagtaaac
    41641 acgctgggtg gctacggtca tgttattcag ccagtgctta ccgggattct tccgtatctg
    41701 caaacaccca caccaaacag tatgtatatc agttggcata gcgaccagac cgcttcaacc
    41761 actgtacagt acggaatctc tgaggcttta ggtctttctc agaccggttc ttatgaggat
    41821 ataagcggaa atacctggca cacggtgaaa cttaccggtc tcatccccga aacacaatat
    41881 ttttataaat gtattagtgg ttcggaagag tcggaattat atacttttaa aaccccggca
    41941 ttgggtgtgc cgcagaatgg ccatttccgg tttattattg tgggcgacag cagaaccgat
    42001 gtagctaaaa ccacagagat agcctatgca gcaaaaaata aagccataga acttttcgga
    42061 gatgatattt acaaccagat taatttggtc tgccatgtgg gcgatatagt gtcgagtggt
    42121 acagaaattt ctcaatacaa gaacgaatac ttcggtccct atgcttgctt gtcgaatgtg
    42181 attcctttta tggtttctat cggaaatcac gagaatgaag caagcaatta ttatgactat
    42241 atgaaatatg aagattttag cgattatacc agttttttac tggaagaaaa attctactcg
    42301 ttttacctga gcgatattca gtttattatg cttaatggta acggattggt tgtaaacagc
    42361 atacaaacag gttggctcga agataaacta agtcagtctg atgcctcgcc aacaaccgat
    42421 atggtttttg cttttgtcca ccaacccgga cacagtgaac tttggcctga tggaaatact
    42481 gcctatgttc aggaagatgt gcttgatgca cttaaacaga ataaaaaagt tcagttgctt
    42541 tcgtatggtc actcacattg ttacgaaaga ggcgttattg aatcacaagc cctcgatgcc
    42601 ataggcgatt tctatattat gctttcaggg ggtgccggtt ccggcctcga cagatgggga
    42661 atgtatccga atcagcagga ttaccccgaa attcagttgg cattggatca ttattgcttt
    42721 agtattgttg atgtggattt ggtaaataaa tcgtgggatt tgtattcata tagtttgggc
    42781 aacctcgaca aaccgttgga cgtggttctc gtagatcaat atcatcgtaa attgctgcag
    42841 gacgctccac tggctccaat tgccttgtct ccacaaataa attcaaacct gcttccattg
    42901 cttgttgcat ctgaaatgca aggtgctgat agcctgatga catcgcagtt tcagcttaca
    42961 tctaatccgg gcaattacgg aagtccgttg cttgacgtta aacgcgattg gataaatgtg
    43021 tactgggact ccggtgctcc cgattatttg cccatcgatt tgaatcaggg tatcgattta
    43081 cgcagattgc agatttctgc cccgcttact gcagggaata cgtacggttg gagagtgcgt
    43141 taccgtgacc acaacctgcg atggagccca tggtcggctg aaaaaacttt tacagctcat
    43201 agcgaccttc aggcatatac cgatttctct gctgatgtga cagaaggaat aagcccactc
    43261 accgttcatt tttccgattt atcgcttcca gtcgtaagtg catgggcatg ggacttcgaa
    43321 aatgatggca cggtcgatag taatgaacag gatcctgctc acatttatac ccaggaagga
    43381 gtgtatacgg taagcctgac taccgccaat ggaacggaga ccaaagatta ttacattaat
    43441 gccgaaacca attcgcttga ggaaattatt aataaaggaa gcgataatgt tcaggtccgc
    43501 ccaaatcctt tctatatcag taccgcaatt gacttcacac tgaaaactgc cgggaaagta
    43561 gtaatcagtg ttttagatgc aaagggtaac acaatccgtg ttctcgaaaa caccgtattc
    43621 aatgccggta aacataccgt aatgtggaat gccggagatg ggtctgcaaa caggttatcg
    43681 ccgggaaaat attttgttaa agtaactggg gcgggtatcg atgcagtgaa aagtgtaatt
    43741 ttaatcgata aataatatga agcaattagc tgttgctctg gcattatgca tgctggcatt
    43801 ttatgtaaca gcaggccacc ccgattcgtt gccagacgga aaggtgctta gctattacga
    43861 gaatggcaaa attcgttcgg ttactcatta taaaaatggt cagcttcatg gcaaatttga
    43921 gtcatactat gcgaatggca ctattaagga aaaaggactg tataagaaaa acattcagga
    43981 gggtaaatgg attaaatggt acgaaaacgg taacctcaaa agtgtagcag aatttacctc
    44041 cggaaagata aacggtgaat accggtttta ttacgaaagt ggagtgctgc agtttatcga
    44101 aaaatacgat atgaataaaa agaacggcga ttggttttac tattacgata caggcgttct
    44161 gaaagaaacg cagaaatacc agaatcataa aaaaattgga acatggtatt attattatcc
    44221 tgatggtaag ctgcagggtg aaattttgta cgacttgagt aagaaaaccg gtgtttggaa
    44281 agaatgggac gaaaacggtg tcttacggct ggagacaccg tataatagta acatgatgca
    44341 gggagtaaaa aagaaataca atgaaaaagg tgttttaatt gaggaaacca cgtacgacaa
    44401 aaacctgaaa tccggattgt gggttactta ctattccaat ggaaatcccg aagtgaaagg
    44461 atattataaa aatggtgaat acaaagacgg cgaatggcaa tattttgatg aaaacggaaa
    44521 tttgaaaaaa accgaaactt acaaagctcc ctcaataata aagaaatgat ggtagctatt
    44581 gccgtttctt aaaatcttca atatactgaa gtgcatactc ctgaaggcaa attttccagt
    44641 cgcgcatata gttaataccc cgtttgttga gttttaggtt taccagtttt tcggaatacg
    44701 gacgcggagc aaaatactct ttttcgaaat aatccgattc aacctttgtt attttaattt
    44761 tatcggaaag ccccagtata tttatgagtt caacagctac gtcgtaacgg ctgcaagagc
    44821 catcgcatac ctgattgtat aaaccgtaat aaccggtttc gagaactttc gatatagcgt
    44881 ttgcaaagtc gagtgtatat gtgggtgtgc cgagtttgtc gtcaaccaca tgcagttcgg
    44941 ttttcccggc aacgatttgc ttataaatct tatttataaa tttcttatcc ttacggggtc
    45001 ctccacccat catccatccc ggcctgaata cgaaacactt cgaaaccctt tccagaacaa
    45061 acctctcccc gtagtatttg ccttttgcat aatgactgag tgggtttgga gtatcgaaat
    45121 cagtaaaata ctcctgttcg ccaccaaaaa taccagctgt ggaaatatac accatggtgc
    45181 aaccgtattt ttcggctagg aaggcaatgt tttctgttcc aagggcattg gtacaccatg
    45241 agttttcgtg atttgtttca caatactcca aatcggtaag ggcagcaaga tgcagcacta
    45301 tttcgggctt gtattcatcg aaaattttta cacactggct gaattcgcgc acgtcgagat
    45361 acgacaacca cgattcgttc aggtcgatat cggtggcctt tacttcctga tatttttcgc
    45421 agaacacttt gtacatcgat tctccaagca tccctccgca tccggtaatc agaactcttt
    45481 tctgttgttt cataaaagaa ttattattgt tttacaacgc tgtctaagat atgtaaatat
    45541 ttaggtttat ttgcttctac cgagaaatta ttcatcacat tttgcctccc ggcttttcca
    45601 atacgttccc ggagtttttc atcatcgatt agcagcagaa tatacttcac ccattcttcc
    45661 gtggtatctg ccagaaaacc ggtaacaccc tgctcaaaat tttcgaggtt cgacccaaaa
    45721 gctgtagcaa cgcagggtat tccgcacgat tggtaagtca atcctttcaa tccgcttttc
    45781 cctagaaccc attcttcttt gggagtagga tacaagccaa tttgcattcc atgtaagtcg
    45841 gcaacttcgt tttgttcagt ccagcggata ttttctattt gtaccccttc gagttcgaag
    45901 tcgcggtcac agataacgcg taaaataatc ttgcgttgtc gcgcaacttc ctgaattgcc
    45961 tcatcgagta agtgcaaaaa tgccatcgta ctatggcttc ctgtccatcc taaagtaacc
    46021 acatcgcgct tttcgtactc tttcagcggt ataaaccggt tggtatcgaa tgttgatgaa
    46081 atatcggtga cgtttttgtt gtgttttaag gcttcttcct caagatattt tgtacagaca
    46141 atcacatgtt ttgcaatttt catcagggta aatattttct ttttcccttt taaaaatgca
    46201 ataaaccagt tcattttact tttatgccct ttgaaaaccg catcatcgat gtcgtaaact
    46261 atgtggggat ttgccagttt taccagccac tcgaacattg accctccaaa aggcgttacc
    46321 caaagatgaa tatataccag atcgtagaaa ggggctctga aaacatcagc gatacgcctc
    46381 atatatcccc atagtgtcag tatgaatttc gtgaaaaaat atccgttttt atacaataca
    46441 gcccaaagcc tgtgcgtcat gaaaggagca attgtaatgg aataaccggc ttcctgccag
    46501 tgcggaaagt actgctcata cttaagcctc tggctgggat acaccttttc gggtgcagga
    46561 caaataacca gtattttctt aattcgggtc accggtaagg gtattataaa tgtttttata
    46621 agaagcagct ccaactttca aatctaatat ttttgctgct ctgttgcgaa tgtcttcttt
    46681 gtttttttgc aataactccg gaatttgcat tgctgccatg cgatatgcct cttgggttgt
    46741 atcgggaatt acaattccgg tttgggtttc ttcgacaata ctgtcaacat ccccgacacc
    46801 tttgttcaca atcatcggaa ttcccatagc cagcatttcg ccttgctttg tcgggctgct
    46861 gctttttttg gaataagaag gttttataaa cgaaatgccg atatcagctg cagcaagcaa
    46921 accgacaact tcgctgcgtt ttgcggaaat caaggttatt ttaccgggtt ctattccagc
    46981 tttctgggct tcctgattca gccagtctcc cgatattatc aaaaaacggg cattggggta
    47041 ctgatgaagc acttccgaaa agaaatcgaa catttcctgc accatgtacc atgtacccaa
    47101 cgaccccagg taacaaagca cgagttcatt ttcggtaaaa cccaactttt tccgggagtt
    47161 attgcgttgc gttgtatcgg gaatacgaaa aaaatcgaaa tcagcacagc atggaattac
    47221 cgatatgttt cctgcgtcga actttacaaa atcccactgc tgcatttcgc ttttggcatt
    47281 gtgtgtgagc gaaacaatat gatcagcggc ctcgagatac tgcttttctt ttcttttgaa
    47341 ataacggtaa acggttttgt acacaggatt tttcagattc cacaacccac cgtcaacccg
    47401 ctcgtcagcc cagaaaccac gcatgtcgaa caaaaattta gctccctgct ttttctttag
    47461 catcaaaccc gccattggtg aaatgtaact ccggcaatgc acaatatcga aacgcatggt
    47521 ttgcataagc ttttttactt ttcgacgaag cttgaatata tcgatgaccg tggagagaac
    47581 cggtgggttt tttgtgtaca caacgatttc ccatccaatt cccgatgaat tgagcaattg
    47641 cctgataaat tgttggttct gtgcaaactt ctcaggtttt tctgtactca atatagtaat
    47701 attataccca cacaaagcca gttgctgcaa atagggtatt acctgcgact gccccagggg
    47761 gtcggtcata ccgtcgtatg aaatataaag cacgtttttc accggcagaa agtaaaactg
    47821 caaagttggg aaaataatcc tgaattcctg caaactgagt cgctttatta accaatcata
    47881 aactcaacaa gcaaatgcat cctatttata aactgtcgga agcagctgta attatttcga
    47941 gagaaatttt ttaatgaatc ttgcgattaa taacgaaaaa attttatatt tgtataatac
    48001 attctataca tatatcaata tgaggtcttt aatacttata tcgcttgtta tactgggcac
    48061 tatgggaata gctcaggaaa acacagccaa ttactggtat tttgggtatc aatgcggaat
    48121 tagttttgct acgggtgtac caaagggtgt tacgggtggg cagattaccg gtctggaagg
    48181 gtgtgccaca gtgggagata tgcaggggaa tctgttattt tataccgacg gtcaggtcgt
    48241 gtacgatcgg aatcacaata tcatgcagaa cggaaccgga ttgatgggac attatagttc
    48301 aacgcagtct tctattattg tcaaatgtcc gggaaccgaa aactattatt atctgtttac
    48361 caccgatggt gccgaaaatc atctggaggg aggctggcgt tattctattg ttgatatgag
    48421 cctgaatggt ggacttggtg cagttacggg agtaaaaaac attcttttgg aaggaccggt
    48481 tgccgaaaag cagacggcta ttatgcactc gaacaatgtg agtgtatggg taatttctca
    48541 tcgctggaat tcgaacgagt ttgttgctta cgaaatcact caggagggaa ttagtccggt
    48601 tccggttgta agtgcaattg gtgcagtgca tgaaggtgga ccgtcaggcg acccgaatct
    48661 taatggctgg accaatgcca cagggctttt aagagcaaat aaagagggtg ataaaattgc
    48721 gctggcaatt cagattacag gtatttttga agttttcgat ttcgataaat cgacaggcgt
    48781 tgtatcgaac tgtaagacct cttctgcata taaccccgtg tatggtgttg agttttcgcc
    48841 ggatggaacc aaattatatg ctacggtaaa tgtgtaccag aattcacgtt tataccagtt
    48901 taacctgaat caggtagatc cgttttcgaa ttcggtattg attgcatcag aatcggacaa
    48961 tccaataaaa agccttcaga tgggtcccaa tggcaaaatt tatattacac gatacaatta
    49021 tggatatgtt tctgcaatca acaatccaaa tgcaaccggg acagcttgcg gatatatttc
    49081 aaatgcggtg tatctaatgg gaaaaacggg gagaaatggt cttccgaata ttttctacta
    49141 taaaggattc cgttttttca caggctccga gcaaaccatt tctatctgcg aaggcgatag
    49201 tatttatctt gagggagctt atcagcacga agcgggtact tttaccgata cactacaaac
    49261 agcgctcgat tgggacagca ttgttaccac atacctcgaa ctacttccgg ctcccgaaat
    49321 acccgttatt acggaattca acggagtttt gtattgtact cctgcagaca actaccaatg
    49381 gtaccgcaac ggtaccccga ttgccggtgc cgacaatcaa ctgtaccaac cctttgtttc
    49441 cggatattat acggttgaag tcgtttgggg aaacggctgc cacagtgtct cagagggttt
    49501 taactttatt gttcagagca tagtttcagc ctccgacgaa aagagaatga tatatcccaa
    49561 tccctgtgga acgagttttt ctactgattt ggaagaacag tattccctga aaatcttcga
    49621 tattcacggc aaggaatgtt ttacacggga aaacctgcag ggcttggttt ttcatgattg
    49681 ctcagtgctt gaaagcggat tgtattttgt cgagattcaa acccatgaca ccgtaattgt
    49741 agaaaaaatg atgaaagaat aaaccggttt attctaccac caacttcgta aaagcgaatc
    49801 cgttttctgt tttaatcttt acaacataga tgcccggcat taaactctca atgtttattc
    49861 cgtctgttaa aaactctgta atatccaaca cgacttttcc actcaggtcg aaaaaaataa
    49921 tcccataaat gttcttattg tcctcaataa aacaaacagt ccctgccgga tttggatata
    49981 gccttaacgc cttttcaata gtctgttccg gaacgcttgt ttcaatatct acaatattga
    50041 ttcgaacatt tttgctgtaa tcgcctaaac tgacgcttag attttctact cccagactta
    50101 aaagtgtaac attttcatcg ctgaaactta tgtgcccgtt tgtttcgtcc caatcgaatg
    50161 ccacggatgt tgagcctgta gaaatgcccg tggtcactgt aaagtttttg cttacactat
    50221 caacatgatt gtaggcttct tcgctgtcga aatgaaggta aacaggtgtt gctgcaacca
    50281 atgccaaact atcgttttcc aaacccaacg gaattgggta acgcccctct gcatacgacc
    50341 agccatcgcc gagcattgcc tgaagttcgg ggcttgtacc tgttagttgg gtagtgagtt
    50401 tgccctcggc tgaacctgaa atatccgtac tatcaatgcc tttgctgagg cacatttgtt
    50461 tgtcgtagta gttgttggtt aatagccctg ttgtatctag acatgttatt gccgaaccat
    50521 aacagagacc tacatttaaa caatttgtaa ttgtatcgtc atttcgattt ccgaaaattc
    50581 cggcaacatt ttgaagtccg ttaatatttc cactattaaa acagttgttt attgacgaac
    50641 tccaaccaaa attgtctcca actattcctc cgatattgtt acgaccatca ataattccat
    50701 aatttgaaca attgctgatt gctgattcga taaccgatcc tgctatacct ccaagattat
    50761 ctattttgcc cgatagattt gcataattct gacaattagt aataggcccg tttgcgtgcc
    50821 cgcaaatgcc accgccgccc acgattaaat cctgaccggt aaactcaaag gaaccgaaat
    50881 tcttgcatcc tgaaatactt ccggaattat tatagcagat tccacctgca tttaaattgt
    50941 tcaatccctg tgagttatag atttttatat tcccgtaatt ccggcaattg attatactcc
    51001 ctgctgatgc acagcaaatc ccccctaaat agtacccgtg tattgtggca taatttgtac
    51061 aattttcaac gatagcaccg gtaacattcg atctgcaaat tccacttgca ttaggggttg
    51121 ttccaagaaa attaagattc tgataattat attctgtttt attaatgagt gagttaactg
    51181 ttcctttgtt ttgattaact atgccgcaca tggcataaat gtatccttca actgtaattg
    51241 aatcaacata tgcctctggg ctaagtatca taaacaaggc ggtataataa tagctgtcgt
    51301 ttctgtagtc aagagctacc ctgatggtat gaccatgccc atgaaaataa ccgttaaagt
    51361 ccagatttaa ccttccaatt ggcgtcctca gcgaatcggt aatatcgttc atcaacgaaa
    51421 agtgtgtgcc tgtgtaatca acggttatcg aaagactgtt ttgtaactct tcaaggtgtt
    51481 ctttactgta aatgcggtag gggtcttcct cggtaccgga gcctccgccg aactgggcga
    51541 aagccatggt tgttgaaagg agtatgccga ggatgagaag ttgttttttc attttctttg
    51601 tttttaggaa tacagaattc aggagacaga atacagaagg gggcagttgt caattcgaaa
    51661 gcaagtgcct ccggcttgat attgccagat tctctcggat cggcaggctt ttctcgcccc
    51721 acgaaacgct ccttctgtat tctgaattct gtcttcttca ttctgtattc tgcattctta
    51781 ttttgtaact ttattttagg aacatgaact ttttatcgaa ttattatagc tttccatcaa
    51841 cttactgact tcttcagcca gtacttcgag atgtttgcta ttaccgtatt ttaaatcctg
    51901 agcaagaata aaataatagc ggcactcttc aagcgaaccc tgagcgatat ttatgaatcg
    51961 cattttgtcg ggttttccac gttttttaaa gccctcgaca atattagcag caaccgaaat
    52021 aattgccctg cgcagttgag atgttagccc aaacatttct tcttttggga atgattcgct
    52081 ataacggtaa acctccagaa ccaattgatg cgctttttgc caaactacta aatcctgaaa
    52141 gttttgtgag ggttttcgtt ccatggtatt gttttttatt gtgtcatttt ttttggaata
    52201 cagaattcag gagacagaag acagaagggg gcggcattca attcgaaggc aagtgtctcc
    52261 ggcttgatat tgccagactc tctcggaaca gcaggtttcc ctagctccac gaaacgcccc
    52321 ttctgtattc tgaattctgt cttctgcatt ctattgttta ttaaatttca atgttgcgat
    52381 gtttgtattg ccgtcataaa ccttcaacac atacaatcca gactttaggt ttcctataaa
    52441 aaactgagtt tgttccgagc ttagattgtc atttgaaatc aattttcctg taatatcata
    52501 aatctcaatt ttgtctgcat ctattccgtt caaatttaag tttatgtaat cccctgccgg
    52561 atttggataa ataaccccat caggtaaaga aaatgtgtct tcttcaacat tactgaaatc
    52621 gaaatttcct acgtcaattc ccgaaatctg actgttctcc cagaaaatca actcttgcct
    52681 ttcggctccc atagtagtgg aggttacaat aaatccgttt tcgcgttcta cccaatatat
    52741 ttgattgcat tcatcattaa ttgtgattgc accaattacg ttgctgccct gatgtatttc
    52801 gagcaggttc gtgcctgtat agctcacacg ccatactttt tcaccggtgc gctcggtaaa
    52861 atacacatac aaatatttta tatcgaggca aatatcgaag acattgaggt tggtttcgag
    52921 tattacggta tcttcgctgc cgtcgaggtt ggcacgatgt aattcatgag cagccatatc
    52981 tgcccagaaa atcagctctc taacggtatc gattccaatc cccattacaa gccctgcatt
    53041 ggtaattact gtctgtaaat cggacccatc cgttttacag cttttaatgg tgttttggta
    53101 gttttcggag aaatataatc gtccgttgtg agggtttatc tccactacat ttatgttgtt
    53161 tgcagtattt atcacatctt cgagttcact gccatcgtaa tttattcttc tgattttgtt
    53221 atcataactg gcagcccagt acactttttt ttgcgtatca tcggtgcgca cccgacaaac
    53281 gccaacttgt ccggtgcaaa gggtgtcaat ctccgtcccg tcgatgttgc ttctaacaat
    53341 aagcccatcg acgaattcac cccagaacaa cagattttgc gattggcttg tggtataaat
    53401 cagtgagatc aggctgagga tgagaagttt ttttttcatt ttcttatgtt ttttggaata
    53461 cagaattcag gagacagaat acagaagggg gtggtattca atccgaaaac acttgcctcc
    53521 agcttggtat tgccagattc tctcggagca gcaggctttt ctcgccccac gaaacgcccc
    53581 ttctgtcttc tgttttctgt attctgcatt cttccttctg tcttctgcat tcttgtattt
    53641 aatttattca acatcaaaca cttcatttac aaataattcg tttactgttt tttcaaaata
    53701 ttgcgatagt ttaaatgcta agggtaacga tggcaaatat ctgccagctt caatggcatt
    53761 gatggtttgc ctcgaaacgt tcatggcttt ggcaagctct tcctgactga gttttcgtat
    53821 ttttcgttct tcgcgtatat agtttatcat gttattcgta gcgattgttt gagtttctat
    53881 agttttcgcg aattttcaga atgtaaaagg ccagtatata cacaaaaatt atcgaattca
    53941 tgagaataat tgccggaaag ttaatccatt gcagtgtaat actcgtaaag gtaaatacaa
    54001 agaaaaacac tgcatgtaac atgatactga aaaacaccgc cttagttcga aaatgctgca
    54061 caagctcgtc ttcgatgtgg tctttcgaaa atgctatcat aaaaaatcca agtataagaa
    54121 tggcgtgtat ggcataaacg cgaatgtcgg cgtcaaatct aaaaaatgta ccgaacggtg
    54181 ttccattggt aagtttcaaa atggccatcg caagcgcaat cagcacaatg gctagcccga
    54241 taaatcgaaa aacgaatgga agaaaaacga tacgggattt gaacatatta aatgaatata
    54301 aactttttac aaatgtaaag ttaactttac atattaacaa atttatttta gcctgaatcc
    54361 gaaattctat tcaacaatca gttttcgcat tacaatgcca gaatctgttt ccaacttcac
    54421 cacatacaca cccgcgttca gattttcgac attgatttcg ccgccggtaa agtcattttt
    54481 cacaatcaac tttttcccgt tcaggtcgca tatgctcaac ttggcattgg gggagtcgaa
    54541 cgaaactgag actgttttaa gagcagggtt tggggatagg gtcaattgcg tgaattcaac
    54601 cggcgatacc gcataaaacc cggggtcatt ggtagcaata ttcgacaaaa ttgagtttcc
    54661 tgtttttgcc agattacatg gcgttgttat ttctattttg gcctgataat acacataact
    54721 gccggtaggc acatcgttat cggtgtattc ggtagcatcg gcaggcagtg tcgtcagcca
    54781 ttgcaggttt tccgggtcgg ttcctctgaa tatctgtata gcagcatatt cggtaccctc
    54841 atacggctca atttgtaggt gccacgacgt aggactcatt atttccctgt tgagccatac
    54901 cgttttgtgt tcatcgctca tgtcggtttc ctgtccacaa ttatacatgg ccgatatttt
    54961 atatcggtac gattgcagtt cgggttgcga cgtaacatcg gtaaattctc caagagcatt
    55021 atagggactg agtcctatca ggttgtatat gccatcgctt tcgcggaaaa cctgaaaaga
    55081 atcaataata tctgaaacag gcttttccca aagtatgaga tttttatttt cgctgttcac
    55141 tgttaccatg cataacgata tcgtatcatc gggtataact gtaacaagga ctgaatcgat
    55201 atcgacacag ccggtcagaa gctctgtaat ggatacataa tacattgtgc tgaccaaggg
    55261 taacgctgtt ggattttcga tggtagcatc atttaagccc gcttccggtt cccacagaat
    55321 tgaatattcg gtttcactgg tttcaagatt cagataagca ggagtgcacg acgatacaac
    55381 ctgattgctg catgcctgaa caatctcatg gttcactaac tttatcacaa ccgtgtcgct
    55441 gctattgcag ctttcgtttt ccaggaacca tataaactga cattcctgag aatactgagg
    55501 tgtaattatt ggggaaacag ggttgtgatt gtcttcaata attatcccat tgcaattata
    55561 taaccaagta cctgaaatct cagaaggttc tgcgttcaag tgcccaatta atgaacaaaa
    55621 catggtatcg ttgccggcat gggcaacagg catttccagg aagttcactg taaatggccc
    55681 agccgtatcg gcacaaaatc caggctcttc ttcaaataca agggtttcaa caatccagta
    55741 aaagtcgtga tatccgtatt gggttaccgt aaccgtatcg aaatcgggct gacttgatgc
    55801 attaaacatt gtgaatgggt tgctggtgtc tacccaatga cccgattgcc ctaagctggc
    55861 aggttcggcc attaactggc tgtaaaccaa tccacaagcc aaagtgtcga aatcggcagg
    55921 ggttgacaat atttcagcac ttggatgagg ataaaaagtt acaattactg tatctctgtc
    55981 ataacatatt ccattggctt cctgccagaa aaacatcttt ggtccgtatt gtgatgttac
    56041 aatggttacg ggagtcgtct gactctcaaa attcgcaccc ggtgtgttaa accaactacc
    56101 ctggtatccg gttccggtgg catgcagggt tgttgtattt ccgcaaacat tgaaatcctg
    56161 tccggcatca accaccggaa tttgcagaaa ctgaatgcta acagtgtctt tcgacaagca
    56221 ttcaggatta tttgtatttg tttctgtcca gataaattca tatgtccctt gctcggaaac
    56281 cgtcactata gtttgattac tgttttgagc ataaaaagaa acatctaaat tcggtcctga
    56341 aaaaacactc cagtttcccg atgggctgta attgctgctt tcttcgaggc tgtaaaatgc
    56401 atgcaggttc ccttcaagac cacaaattgt ataattatat ccggcattgg cggttggcac
    56461 ctgataaaag tggacattaa ttgtatcgat gctagtacaa ccgaagttgt tcagtaccca
    56521 aagaaattga gcctgcatgc tcgctgaatc accgaaacag ccatatggat ttatagtaac
    56581 tgttgcatta gggtcgtgta cattgtcgaa gtttgacatt aactgactga tccatgtgcc
    56641 atatgcccaa cccatcccca cagtgtctgc cgaaaaagta taggtattcc cacaggtttc
    56701 gacgtaatcg catggcccag cacctgcaat cggctcacgt gcaaaccgga ttcttacggt
    56761 atcagcgcat gggccgctat tgtcggtcca cacaaaatca acatcaaaaa acaacccaga
    56821 gtaaaatgga attaataccc aggccacagg atctgatgca tgggcagtac cgacaaatgt
    56881 taccaccagc ggggtggcat tatgaaaagc tgtccattct ccggtagtgg tgttttcggc
    56941 atgaagctgg tatgccacac cacaagtatt atcatcaggc ccagcatagg ggttgctgca
    57001 ttgtgcttgc acaaaggagg tcgtgtatat caggctaaca attaagaaaa tgtgtttcat
    57061 aggtcgtttt ttttagtttc taattctgca ttctgcattc ttatggttta aaaaatattt
    57121 tcccagcagc tttatgttct ccggcaaaac aggcaactac gtactcaccc ggtttttcat
    57181 tttcgggcgt ccagctaaaa gtatattttc ctgaaccgtc agtcataagt ttttccgaat
    57241 gaacaatgcg tgcatttatg tcgtatatgc ggaattctag ttcaggctca ggactgacgg
    57301 cttcaatgcg gatgctattg gcgaacgggt tggggtaagc agtcagatgg attggtaatt
    57361 cctttattgg cacaactccc agtggatcga atttgtcgtc gcgcatataa tagattgaat
    57421 atccttcgtc ggcttcctgc atccatataa agtccacaac ggttgctctg aaatgcttgt
    57481 aacaaatcga aggattgaac ggattatttc cgatggtttc aagaatcgga cttgtgtcgt
    57541 atccatacga aaatgcgtgg tacagagtgt tgtttcttaa aaacaagtag ctatatccca
    57601 gggggggtaa aatgtcgtct atacaatgat aatcaattgg ccccgattga attatccgtt
    57661 catcattggt attgtattgt tctatataat ttttttcaac caattgttga tcatagtcgg
    57721 tatatagaat ttttaccgta cagtcatgat ggtacatatc ataccaatat tctgtattat
    57781 agctgattgt tgcaaaagtg ccgaacataa ctccttgtaa cggtaaatcg ccccaggttt
    57841 ccatctggtt agtactacgg tacaaaagag taaacgcgtc actcatatca acatcataac
    57901 ctatacctat tgcaaaatat actgtattgt tgccatattg taaatggcat gccatggcac
    57961 aatcacttac accttctatc aactcagttt tctgaacggt atcttcaacg agccgtgcgt
    58021 attccagcaa atgatcgtaa agtgaagggt tgtatatttt ttgccaaaac acatggacat
    58081 tgcccgcaga gtccacgtct gcaataggga aacgacaggg aatagaaccc tcactgataa
    58141 ttaattcttc tgtccaagta ctgaaatcca tagaagaacg gtaagccacc ctagggacaa
    58201 tatcattggt ttcataaatc agaaaaaact tacagccgcg gctaatagca aatgtggggt
    58261 ttttcccgtt gcaaattaac actggttcgc tccatgtctc tgtttcacgg ttcagaaaaa
    58321 gtccctttac caccatattt ccatcgaggc tgtcgtgata taccacatac acattatcga
    58381 gtgtatcgcg aacaattttg cgctggtttt tattggctgt agcgtcaata caatggctgg
    58441 tagcaatctg ggtttgccct tgcgtacaaa ccgaaaacaa caggcacatt gccacgaaag
    58501 cgagttgctt cttcatagac ctttatattt agttttatac cccatgaaat tactttccta
    58561 ttctacaatt aatttcctta caccaacccc cccgtccgtt tccagcttca ccacatatac
    58621 gcccgctttc aggtgttcaa tattgatctc gccgccggca aaatcgttca acgcaatcaa
    58681 aacttttccg ttcaggtcgc agatgctcag actggccttg ggggtatcga attgaatgga
    58741 tacgttccct gaagttgggt tgggatatat ctcgaactgt aatggtgaag cattgctgcc
    58801 cattccgtag aatccggggt tgttggtggc aatattcgaa aagatcatta catcgtcttt
    58861 tgcccctgag caatctgccg tggtattaaa accaagtcgg taatagcaat attctgctcc
    58921 ttcgggtaag ttatcatcag taaacgtacc gacatcgtta ttcaccgatg taagcaattc
    58981 caagtggtaa ggcgaaatgc cacggtatat ttccgttacc gaataatcca gcccaaagta
    59041 ctcattccat ataagctgac atgtgctttc gttaggtgag tttacctgaa gatacacact
    59101 gcgatggttg ctactgagaa ctgtttcttc ggagcagttg ttgataaaag agattttata
    59161 atttgacgat gctgcccatg gttgtgccga ataatcggta aactgtgctt gggcataatc
    59221 gcatgctgca acttgatgat attctccatc ttcctcacgg taaatatata cggaatctaa
    59281 ggcagcttga ggcattcccc aacgaatgag gttgccatac aaccccgatg aagtaacata
    59341 acagatatgg ggttccggaa gtgggcagca ttgcggatca agtttatcga ggataaataa
    59401 aattattgtt gtatcgtaaa cacaaccaaa attatcggtt atgtgaaata cgtatggaaa
    59461 tccgccacca gtaaagttgg gggtaaaact tgtagtattc ccggagtttc catccagaca
    59521 ataacctgtc caaaaagagt tgtcactgct ttgcgacgac cactgattct gcggatattc
    59581 cggtatttcc aagaagtcgg tgtattcccc cttaaggtgg atgtttgccg aaaatataaa
    59641 gccatcatct ccgcacctat tgtccattat aatcaattgc cattcaccgt ttaccggaca
    59701 gccatataaa tcgtcgaagg tctgcaccgg acgatagcta cccgcaggaa tattctgata
    59761 cgaagtgttt tcgatgtctt gaagggaaat gcttgcctca tccgaccagt aataggtcca
    59821 tcccactccg ggaacacagt tgtcatcatc tgtcggtatt ccgtaatttt tcccgccaca
    59881 ggtactttca tcaaaaatac gtacggtttt accactcgga cacataagta acatttctaa
    59941 atctcctgct gcactgtgtt ccatttttac ctctaaagag cttaaatcat cgggttcctg
    60001 caatgtggtt atgttcgaac tggtacttac ataaaacggg aaatgctgat aatattcata
    60061 acaaccataa atacaattat ttattatatg gtcgttatcg tttgcaaccg ctgtaacagg
    60121 ggaaatatag cctgtaagct gaacttcctg cccctcgcaa gcagcctcct catggcccgt
    60181 gatttcgaaa atcggatcgg gcggaactct gacaaagaga acaggcaggt tgttagattg
    60241 acaagtatta aaatcgctgc ttttcaagag aatcatgtac acaccgggat tgtcaaaagg
    60301 aatagcaatg tcagtcaaat tcattcctga atactcattg aataggtcga ttaggaacat
    60361 cgtgtcgtta taaaccaacc atgtgtattc tgttgtcgtt gcggattgtg ggtaatttcc
    60421 gttttgcccc gactgaaaat tactttcaac agaaacctca agggttgaat tatggcatac
    60481 atcaatgtaa ctcccattgg gcaataattg acctgctgca tcgaaaagcg catttgtgta
    60541 agcaatgcat tcgtatttac atgaaatacc aattgcaaaa tcagcatcat aagaatatgt
    60601 ggagacccat tcaattgtaa tacaattacc cgttgatata aacgatctgt tttcttcgac
    60661 cggtgcataa ttcgattgca gcggtggaga gttcgtgtcg ggtccgtcgt atattctcaa
    60721 cgtatcaaac atataaatat ctatcatttc gatatttatc aatacatgcg aattggcttg
    60781 ccccgaataa attgttttaa tgaaatgctc tccaggctgg catgaattat tagcccctcc
    60841 cgaatcgtat aaggttccag aacaggttgt aatgattgaa ttttcgctca ttagatatgt
    60901 ttgtgcaaaa ataggcgtac atcctataat tgataataaa atagaaaata cccgagtttt
    60961 catatgcacc atttatgcta ataaatataa aagagaacta ttaaaaagac acataaatat
    61021 acgatttagt tgcattgcga ttacaaaaag tttaaatatt tatctgaagc aaacaaactt
    61081 tcttgtttcg cagagggtag tatggcatga aataattgta ataaatcgca aaatttatgt
    61141 taagtttgtt gaaataaaat agttacaatg ttccgtattt tacttttgcc gttgtgcctc
    61201 atcccttcgt tgctgatggc gcaatttacc gatgattttt ctgacggaaa tttttctgcc
    61261 aatccagaat ggacaggtaa taccgataaa tttcagattg atgcttcaaa ccggttgcag
    61321 cttaacgaaa ttgaatatac cggctcagca tatttatcaa cagcttctca ggcagtgaac
    61381 gaagcttcgt gggaattcct gctgcaattc gattttaatc cgtcgtcgtc gaattatgcc
    61441 agaatttata caattagctc aggtagtgat tttaccggca gcctgaacgg gtactttata
    61501 agggttggat ataccgacga tgatataagc tttttcagac aggatggtgc tgcggctagc
    61561 ttattgattg atggaacgaa tggccggtta aacagcgatg ctgtaacagt gagaatacgc
    61621 ctcgaacgag atgggctggg aaactggagc cttttcagcg atacattggg gggcacaaat
    61681 tattatgccg aaggagtttg cttcgataac acctacaatg caagcaatta ttttggtgtg
    61741 cgctgtacgt ttactactac gcgaaaagat aaatttttct tcgacgattt tgtggtaacc
    61801 ggaactcctt ataccgacaa tgatccaccg gtatttcaat cggttacggt aatctccgaa
    61861 aattcagtgc gggtggtttt caacgaagat ctttctcaag aaacagcgct gcaattcgct
    61921 aattattctg ttgaccacga aatcgggaac cccgtgtccg cttcattttt caatgataat
    61981 cacgcgattg tggatcttgg ttttgcaaat gattttatat cgggaacaga atatacgttg
    62041 caatattccg gagttgaaga tgttgcagga aatgtagtgg tttcagggag tggaactttt
    62101 atatacctga ttactcaacc cggcatggtg gtaattaacg aaatcatggc cgaccccaat
    62161 ccggttgtag acctccccga ggctgagtat atcgagattt acaatacgtt gcaagtgcct
    62221 gtaaatttaa caggatggag gctgatagtc aacaattacg tcagaacatt ccctgcggtt
    62281 gaaataggcc ctgaatcgta tctgctgctg tgtagcacta ccaatgttga attgtttgga
    62341 actgaaatac cggttgctgg aatttcttcg tttcctgcta tccccaacga tggagccacc
    62401 ctgaaacttt tcgatgccga cagtgttgaa gtgaatcagg ttacctatga tgtaacatgg
    62461 tatcacgatg caaccaaaga tgatggcgga tggtcgctcg aaaagataga cccacttaat
    62521 aactgcagcg gtgcaacgaa ttggattgca tcggaagacc cctctggcgg aacacccgga
    62581 tttattaact cggtgaatgc ggtgaacacc gataacactc caccagaggt tgcgatggta
    62641 caactttcgg ggacaaccga aatcgtgata aacttttcgg aacccgtcga ttccattact
    62701 gcaaccgatc ctcaaaacta catgataagc catggttacg actctcccga taatgttgaa
    62761 acggatgcaa ccgaccctgc tgtcgttcat ctttttgtgc agactgcttt cagcgagggc
    62821 atagtgtatt ctgtcagtat agaaggaatc tctgacctct gcggtaatac aatgcttcct
    62881 tcgcaacacg atttcagtat ttacacgccg gctgtaaacg atatcgtcat caacgaaatt
    62941 atggccgatc caacacctgt ggtaggcact ccggaagcag aatatattga attctacaat
    63001 acaaccgaaa atcccgtgac tctgaccggt tggtcgctgg ttgtgggaac ttatatcagg
    63061 cctattcctg ctgcacaggt tgaagcgcac ggttatattg ttctttgcag cacgggaaat
    63121 accaatcttt ttaatacggg tgttaatgtt gtaggaatag cgtctttccc tgcagtaccc
    63181 aatagtgggc aaacgctgaa aattcttgac ccgaacggaa acaatattaa cattgtaagc
    63241 tatcaggata cctggtatca ggatccttca aaggatgacg gtggctggtc gctcgagaaa
    63301 attgatccat tgaataattg cgttgaaatg gcgaattggc gggcatcggt taaccctttg
    63361 ggcggaactc cgggtgcaat aaattcagtt tatgcttcaa atcccgataa cacaccgcct
    63421 cacatacaag cgctgcaagt gataaacaac gcagaattgc ttcttgagtt ttccgaaaaa
    63481 gtggacagca atacaatact gatttcaaat tttttggttg agcaattatc ggttcatcca
    63541 acgcaagtct tactcaattc agatagtaaa acggtaagcc tgacttttgc cgatccgttc
    63601 gaagaaaaca ccatatatac ccttgaagtg agtaatgtgg cagatttatg tgggaatgcg
    63661 atggaaacag ttttctccga gtttaatatt tacttccctg tagtcaacga tattgtgatt
    63721 aacgaaatca tggcagaccc ttcaccagta gttggtcttc cggaagctga gtacatcgaa
    63781 atatacaata ctaccgcaaa tgtgattact cttacaggct ggaagttgat tgtggggaat
    63841 tacgtaaaag aatttccttc tgcgcaaatt ggggcaaacg gttatatggt tctttgcagc
    63901 ccggcaaatg ctgtattatt tgaatccgga ataaataccc ttggaatcac ttctttccct
    63961 gcattgccaa acgatggtaa aaccgttaaa atactggatg ccgatagcgt cgaaataaat
    64021 caggtcagct acgacatttc ctggtatcag aattccgaca aaatcgaagg gggttggtcg
    64081 ctcgagaaaa tcgacccact gaaccattgc ggaggtatga gtaactggat ttcctctgtt
    64141 gcagaaatcg gtggcacccc gggtgcagtc aactcggtga tggccgaaaa ccctgatagt
    64201 tcaccaccta cggtagtttc tgttcaggct cttacaggca acgaactatt ggtaacattt
    64261 tcagaattta ccgatacgat tttagctgtt aatccagcaa actatcaaat atcgcccgac
    64321 tacaacaacc ccgaatatgt gtatgtcgac cccaacgatt taagacaagt acatattttt
    64381 gtaccctatt ctttcaccga aattgttgta tatacgctta ctatcagcaa tatatccgac
    64441 ttgtgcggga atgttatggc cacctcaaac attaacttca gcatttataa cccacaacca
    64501 tacgatgtgc taatttccga aattatggcg gatcccgacc ctgtggttgg acttccaaat
    64561 gccgaatata ttgaattaca taacgttacc gatatgcccg ttaacttggc agggtggaag
    64621 ctattctccg gaagctcctc atgtgttttg cctgcttaca caatacaggc gggtgcttac
    64681 attaccattt gtcacacaaa caacagcctg ctttttgcac agtacagcaa tgtactcggg
    64741 gtttcgggat ttcccgccct gcccaattca ggagcagttc tttctctggt taccgacaat
    64801 aacatgaata ttcacgctgt tgaatattcc gatatctggt acaacgataa tttcaagaaa
    64861 gccggggggt ggtcgctcga aatgatagac ccttccaacc cctgcgaggg agaagataac
    64921 tggattgcca gtaccagcaa cagaggaggc acaccatcgg aaatgaattc agtaattgct
    64981 gaaaacattg atgtaacaga cccgttcgcc gaaagtgccg aaattgtgaa tgccgacacc
    65041 gttagggtat attttagcga aaccatgcgt atttcgtcgg tgttgcccgt tctgaattac
    65101 ttggtcgatc atagtgtggg taaccctgtt tgggtgtacc ccgaacctcc attgttcaac
    65161 tcagtgctac tgaaatttaa tactgtcttt gaacccgggg ttgtatatca gctacagttt
    65221 gccgatacct tgcgcgattg cagaggaaac gacattgttc agacttttga agtgcttttt
    65281 ggtattgccg actctattgg gcaaggcgac cttgttatta acgaaatcct gtttaatccc
    65341 tatcctgatg gatacgattt tgtagaattc tacaacaact cagggaagct tctcgattta
    65401 cgtaatgtat ggctatcgca gcgagatgac tcaggaaatc cgggtgagcc tgacagggca
    65461 agcgaacgaa gccatgtaat cttgcccgac gattatatag taattaccga taatccggca
    65521 gatattgcaa caagatatta cgtgccttca ccccagaaaa ttatttattc cgataatatg
    65581 ccctccttac ccgatagtga tggaaacctg attgtgtttg accgtgcaat gaatattatc
    65641 gacgagttca gctatgacgc cgacatgcat tttgaactgc ttgccgacga cgatggggtt
    65701 tcgctggagc gcatcgattt tgtagaacct acgcaagctg catctaattg gcattccgcg
    65761 tccggcgatg tgggttatgc aacacctgga tatcaaaaca gtcagtaccg cccttcttcg
    65821 gttgatgcaa ttaacagcct tgtacccgat cccagggtgt tttcacccga taatgatggc
    65881 tacaacgacc ggttgaatat tctgtatcag ttcgaaaatg caggtacagt cgccacaata
    65941 gcaatatacg actccaaagg cagatttatt acctacctgg caaaaaacga aagcctctca
    66001 gcacaaggtg ttttgttttg ggatggcctt gacacccgca atcagatttg cccgatagga
    66061 atttatatta tctacgccga gatgtacgat gccacaggca aaacatggaa cgaaaaaatt
    66121 acctgcgtgt tgagcattaa aaaatagaaa atgcctctgg aaagcttcta ataatatcca
    66181 aagaattata agttgttggt acaataaaat agatagaaat aattttccat ttttaattaa
    66241 atagtgtttt taattttgat ttatatatgt aattttgttt ttaataatat taagaacaag
    66301 acttttcgat tttttgtatc ctttcttgaa attagaatta atttatatat taaaactact
    66361 agtgatgcgt taagtttaaa aaatgtttta taataattta aatatcaata a
//
LOCUS       DAOOQP010000002        63586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_485_length_63586_cov_6.089736, whole genome shotgun sequence.
ACCESSION   DAOOQP010000002 DAOOQP010000000
VERSION     DAOOQP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 63586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_485_length_63586_cov_6.089736"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1475)
                     /locus_tag="PLK75_00260"
     CDS             complement(375..1475)
                     /locus_tag="PLK75_00260"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HOY37707.1"
                     /translation="MTNTSTIIEFARRNKGVLQNHLADGSLRLPVALEILEKHQLNAL
                     YLLSIETPIDLCSFFKSGLLQLESIINNPQYTHYTIKKKRGGNRHIYAPNKKLKAIQK
                     RLNYFLQAYYLMIKPEEVHGFVVNPHYLGSHCNIVENAKAHTGKKHLLNIDLKDFFPR
                     ITAKQVLDLFSSPVFGYSDQVATAITLLCTYEGRLPAGAPSSPVISNFICLNLDADLK
                     AFCATHNLEYTRYADDLTFSSNAAIETNTLLDIISLIQKNNFEINSNKLRIRPANRRQ
                     IVTGLTVNEKVNVNRKMLKKIRAMLHDLSTNGVETATQKHFKITGKVKAAHEAKFLQR
                     LEGYINFVGQVRGKSDPLYIGYKNQFEQVFEN"
     gene            complement(1503..1646)
                     /locus_tag="PLK75_00265"
     CDS             complement(1503..1646)
                     /locus_tag="PLK75_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37708.1"
                     /translation="MMSKESTTWWLCSCNPYSVEGMNFDYLRGLTPLFTSFALVNSQR
                     VKR"
     gene            complement(1682..2776)
                     /locus_tag="PLK75_00270"
     CDS             complement(1682..2776)
                     /locus_tag="PLK75_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOY37709.1"
                     /translation="MEENLKHQIEKIIASPTSNEREILLRLKQLIYEKEFQNIVVRES
                     ENISGLVEENLTRLLRGVKDYNIIKSGFRDFDITFGGFSQGELVIIGGRPSMGKTQLL
                     INLSLNMAQEHPVLYFTFDLPKPTLVNRFLAAVSGIAAGNIIQQDLSAEQKGKLTAAG
                     KTIAACNIFINDSCSNSVTQLKKMCEKHIRENGVKVIILDYLQLMSSSKYKNNREVEI
                     SYISRELKNIARELNVCVIASSQMSRAVEVRTGEKKPHLSDLRESGAIEQDADKVIFI
                     YRPEYYGMLEDREGNCTEGIAEIIMAKNRNGGTGSIQLMIDKDFTRFMNFTGYKNNFE
                     FSKNRLRSLLSPDSKSCDESGSDSSFDSPF"
     gene            complement(2907..4772)
                     /locus_tag="PLK75_00275"
     CDS             complement(2907..4772)
                     /locus_tag="PLK75_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37710.1"
                     /translation="MVNNIFALFLLFLASPVFCQQWIVREVGTCPNDKTYGICVGNGR
                     NDGINRIYVTTRGEPSDAAIYEWTWSGTTWVMTSTIATGLKNLITIAVGEGRNDNVNR
                     LYAVEWGGTTSRVFEYSWNGTAWVSSLIQTTAKPMLSVTIGDARGDGTTRVYVDGWII
                     HREYTWNGSGWNIHNVSLGHGTEGPTTIAKGRNDGQVRYYVSGNHVKEYTWTGAGYTD
                     STSISAADGWPETVIVTDLRNDHQNRVLTQDNSGIYEYTWNGTVWLQNKISGRFGRSF
                     LFGARPKSDCKLYVYSTDVDSAFREYRFNEQTQMYNFSTIDAATGATALVDAGDGRND
                     DTIRIYTPRYANGKIYEITNVQPYVLEIPPEITGNTMLCPESSGEVSTGIYETYQWYR
                     RYYGSSETLPIEGAVNQNLTIDYYNFAACYLSVEVGYNGESYLSEEFFVDGYTFLPPT
                     IETTGEFTIGTNGETIICEGDEVVFTLNNPYTINIIWYKNGEMIQGEHSQQLTVNSPG
                     LYYVEGAPDECPDYIQGPGVVMEVVFCDPNSIFTGTHSGGVTLYPNPASDILYVLIPE
                     PSDEISFAINDVSGRSILSGKLNSKTTIELSGVVPGLYYLKLGKGSNTIYKFIKE"
     gene            4811..5200
                     /locus_tag="PLK75_00280"
     CDS             4811..5200
                     /locus_tag="PLK75_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37711.1"
                     /translation="MTLIKTGKYFFIFPGFFLILFYSLGCESTYIRMGDEGTHTAPAD
                     TVKLSTHVQPVLYNKCASCHFAGTTLDLESADIYNSLISGNFVDTASPENSRLFSEPD
                     PGHADDYLTVDEHTLIVRWIQQGAKNN"
     gene            5206..6156
                     /locus_tag="PLK75_00285"
     CDS             5206..6156
                     /locus_tag="PLK75_00285"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HOY37712.1"
                     /translation="MVKNILSKFAIIVGIVLYCGLVMGQTDTLPNSAKIHKPVINPWS
                     GSLIINNQTTELSPKNGLDFVIHHRFTSVENGLSDLFGLYSASNIRLGLQYSITNNFM
                     VGFGSEKDNKCQEFFLKYRFLTQTRDGVIPVSVAAYASAAINAREKAYFGTDYRFANR
                     MSYFTELLISRKWYERLSTQLSVSYGHINKVESYRVVSGDTISETVTYYPKFYNDALG
                     LGIAARFKVIGSWTLVGEFEYPMALGKGGRHGNSSIPRKLKPKPDAALGFEVSTMTHV
                     FQLFVSSYRAIVPQYNLITNTYDFTEKSGIMPGFNIIVKF"
     gene            6166..6582
                     /locus_tag="PLK75_00290"
     CDS             6166..6582
                     /locus_tag="PLK75_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOY37713.1"
                     /translation="MKNKNLSKFLIVTVLSAGLMSFLAVELVKDPWNVPAQYKTMKNP
                     VKKEATSIADGKALFEATCATCHGKTGKADGVKSKNLTIEMFDLSAADYQSKFSDGEI
                     YYQSFIGRYRWHDFTKVIPDESDRWSVVNYIRSLKK"
     gene            6807..7184
                     /locus_tag="PLK75_00295"
     CDS             6807..7184
                     /locus_tag="PLK75_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY37714.1"
                     /translation="MKRVTGIGGIFFKCKEPEKLKQWYQTHLGFDINEFGAMFAWRED
                     VDSSKTGYTIWSPFNESTDYFEPSTKDFMINYRVENLEALVKELNREGVTVLDSIETY
                     DFGKFVHILDLEGNKIELWEPTN"
     gene            complement(7194..8213)
                     /locus_tag="PLK75_00300"
     CDS             complement(7194..8213)
                     /locus_tag="PLK75_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOY37715.1"
                     /translation="MSSLFQRLLAGMLQREYIRKGRQPGKHEHIAELQRRQIDAKHFL
                     INCAFIAAGVLVAAFGLKGFLMPSSFIDGGITGISLIISNVSSISVSLLILILNIPFM
                     LLALSTVGRQFAVKSIIAITLLAIAVHFIPYPVVTHDKLLVAAFGGFFLGLGIGLAIR
                     GGAVLDGTEVMAVYLNKKFSLTVGTVILIFNVFIFLSAAYILSVETALYAILTYFAAS
                     KTVDFIVDGIEEYIGVSIITDKPEEVRLAIIENLGRGCTLYMAKNGFAPRGELLKDTE
                     VVYTVITRLELSKLKNEIDKIDIKAFVIMNPIFDAKGGMVKKKPLRKLRQQGKREEEI
                     TKNGR"
     gene            complement(8223..8789)
                     /locus_tag="PLK75_00305"
     CDS             complement(8223..8789)
                     /locus_tag="PLK75_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37716.1"
                     /translation="MKYSFFMLVIVISAILISCGVNKKDKTSESNSNKNDSEFVLTRE
                     TPADFVHKDLSDYGFPLTVMVPPDADVYTSKLITDEGTSDQIVIQLNAASDVRMNIRN
                     SKNTLAQYREDAENDIFKEFDRYVLENDSQVVYKATHDKNDSCFYLVCVVTGANSPFA
                     IISDEFHEYGFNDIQKLYSIANSVKPIK"
     gene            8909..9499
                     /locus_tag="PLK75_00310"
     CDS             8909..9499
                     /locus_tag="PLK75_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HOY37717.1"
                     /translation="MTRIGRQMPHFEEIDLNSISAEFSADRKYRYVLTAHYRNSLLRS
                     DANEQIAVVLKNPSSADAFKSDATIRKVETFVHKHFEKAKTLVILNIFALRATDASDV
                     QQCFLQYGYDYITGDENNQYIAKVLKQSDAIICAWGGNSGIDKTVYNKRIAEVKSLLK
                     SCGTKCYEVKSPAATNEPLHGLMWGYNYECVLFTLY"
     gene            9510..9980
                     /locus_tag="PLK75_00315"
     CDS             9510..9980
                     /locus_tag="PLK75_00315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOY37718.1"
                     /translation="MTELKNPFTRQHDFNCFGCSPDNSNGLHLQFAAEGDKVICEWAV
                     PAGFEGWPGVVHGGITATMMDEIAGWYIFAILGVACMTVNLNIHYEKPLYSRNGKIRA
                     EAVCIERHKRIAKIRVDVSNSDGSLAAWGELKYYCFTEEESKSKMNFPGKEAFR"
     gene            complement(9985..11898)
                     /locus_tag="PLK75_00320"
     CDS             complement(9985..11898)
                     /locus_tag="PLK75_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOY37719.1"
                     /translation="MSDYKNILTKYWGYTKFRPLQEEIIRSVAAGNDTLGLLPTGGGK
                     SIIFQVYSLSVNGLCLVVTPLIALMKDQVENLRKKDIRAAAIYSGMSRDEIDFTFMNV
                     INGGYKFLYVSPERLQTQSFTERIPYLNVNLITVDEAHCISQWGYDFRPSYLEIAKIR
                     TSFPGIPVLALTATATREVVADIQKQLLFPKENVFTQSFERKNLTYTVVQTENKLKHL
                     FKIITNTPGTGIVYVRNRKGTKELALMLQEYKLSADYYHAGLSPEIRSMKQDAWQNDK
                     TSVMVCTNAFGMGIDKPDVRYVIHMDLPDSPEAYFQEAGRAGRDGNDSVAYLLFSPAD
                     ETAVKQRISRSFPEISSIIRVYEALHNYFGLPLGAGRSMSFEFDMFDFAKKFSLDVIT
                     IFNSLKTLQLEGYLEYNDALNNPSKIHFPVKRDELYNFQVEKKEYDAFIKLMLRNYTG
                     VFNDYVNFSEEYLAKKAGTTVDTIYTYLINLQKLNIIKYIPRKNKPVVVLLSERIELQ
                     SVYINKRNYELQKERYITRMNAMLQYANSTNKCRSQILLAYFGQTDAERCGKCDVCKR
                     RNELSLSKYEFDLILSEIKEHIQQKPIFLNDLIDEINHDDNKVIKVIQWLFDNEKIHY
                     NEKQKLTWNQRGK"
     gene            11995..13473
                     /gene="guaB"
                     /locus_tag="PLK75_00325"
     CDS             11995..13473
                     /gene="guaB"
                     /locus_tag="PLK75_00325"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOY37720.1"
                     /translation="MSYVSDKIIGEGLTFDDLLLVPGYSEVLPYMVNVASRFSRNITL
                     NTPIITAAMDTVTEYQMSIAIAREGGIGVIHKNMSIEEQARQVRSVKRAENAMISDPI
                     IINDQATVGDALNLMKEFHIGGIPVVDKNHILKGIVTNRDLRFQNNPSKPIREVMTSE
                     NLITTSQNINHEEAKEILQRYRIEKLPVVDKDYKLVGLITYKDITKAADSPNSCKDEQ
                     GRLRVAAAVSIAANTLDRMDALVAANVDAMVIDTAHGHSKGVIDMLKVARKRYPNIDI
                     VVGNIATGEAAKELVKYGADAVKVGIGPGSICTTRIIAGVGVPQVSAIFDVAQALKGT
                     GVPLIADGGIRFSGDIVKAIAAGADSIMAGGLFAGTDESPGETIIYNGRKYKSYRGMG
                     SIEAMQMGSKDRYFQDMEDDIKKLVPEGISGRVPFKGTVGEVIYQLIGGLRSGMGYCG
                     CKDIDEMHRNARFVKITHSGMIEGHPHDVTITREAPNYTREN"
     gene            13496..15082
                     /locus_tag="PLK75_00330"
     CDS             13496..15082
                     /locus_tag="PLK75_00330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY37721.1"
                     /translation="MKKAFIFLIVLFAACSQIVAQEDVLLEIDNHKIDVNEFLHIYQK
                     NNNDANSMTFDAMMDYMDLFINFKLKVIEAESLGLDTTKEFKVELNGYRQQLAQPYLT
                     DKTVEDELILEAYERMKQDVNVSHILIKVDQNALPEDTLKAYNKVMGVYERLKKGEDF
                     TTLAIAVSEDESVKYNNGDLGWRTVFGLVYEFETEMYKTDIGKFSQPFRTRFGYHILK
                     VNGKRPAKGKYKVAHIMLTIPKDATPEDKVKAEARLNEVQAKINAGDDFAALAREYSE
                     DRQTAGNGGVLGWIDVGGKMIRSFEDAVFGLTEINQVSEPLKTSYGWHFIKLLEKQEI
                     KPFEEVRSELKSKISNSARASRSREVVIERLKKEYNFTLNQKAAAAFYKLVTDSIFAG
                     SWDATEALKLDNVLCEFGDKKLYQKDFADFLDKMNRKQVPQPIVVFVDNALKSFIDKQ
                     IIEYEQSRLEMKYPQFKYLMQEYHDGILLFELTDQLVLQRQLLILRGFRYFTKTTRII
                     TCGLFGMRFININVILQKML"
     gene            15022..15456
                     /locus_tag="PLK75_00335"
     CDS             15022..15456
                     /locus_tag="PLK75_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37722.1"
                     /translation="MWPVRYEIHQYKCNSPKNAVKVEKLLKKGADFDQIVPKLSKKDS
                     TAVVAGTNGKFVKLQSAETDNIISKSGLSETPGKTAVFLTDETTVVMATVIAPEHKKL
                     EEAKGLITADYQNYLETDWIKKLRSKYKILLHEDVLKKVAKN"
     gene            15473..16342
                     /locus_tag="PLK75_00340"
     CDS             15473..16342
                     /locus_tag="PLK75_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37723.1"
                     /translation="MRYTFFQSFCVVSLIAVVMISCGGNDDTKLNVVARVGEKQLLQE
                     DLEFLIPNSFSKEDSLKFAESYINDWVFEQLLYAEALNSITDTASINKQLEEYRQSLY
                     NYYFERQLLESKIDTVVGHAQISEYYSQHLYEYVLDQPVIKLHSLVFDANKIDYIAEV
                     RMIKNTEIDEIESLFEFCERVGGNIMLIDRWIRMSDFYRIFPCEGNLTLEKIEKGGYY
                     ECFNDSLRYLIIIDDIIPVGSYCPQEVLTDDIRKIILQKRSKDFMSIYKKQLFIQAEN
                     SGRLFIKNKKNED"
     gene            16332..17687
                     /locus_tag="PLK75_00345"
     CDS             16332..17687
                     /locus_tag="PLK75_00345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY37724.1"
                     /translation="MKIKIAFFMIILLGTATVRAQEVIDQVVAVVGNSPILKSDIEGQ
                     YIQMMSQNYYSSSVDLKCEILEEYLFQKLLLNQAMIDSIEVTPKEVDTELNRRLGIFI
                     NQMGSEEKLEEYYNKSINEIRDEFRSIIKDQLLTQKMQSKLTTDIKISPSQVRQYFNE
                     IPADSLPIIPASYEFSQIVVYPDLNDEQKQISYDKINELRERIVKGDSFATLAILYSQ
                     DPGSAAKGGELGFVSRTDLVPEFAEVAFSLNNTEVVSPIVETEFGYHILQLIERKGEL
                     VNVRHILIIPEISDVDVKKAKKDLEDVYKFILSDSLSFEKAAAAYSDDEDSKSNGGKA
                     INPYTGNSKFLTDQIDANSLQILNQLTPGQTSSVFETRDLRGKKVYKIVRLDRRIDEH
                     VANLNDDFKEISEVALQQEQFKRIQEWISHQIDITYIFIDDSYANCTFKYADWNKNSK
                     K"
     gene            17692..18150
                     /locus_tag="PLK75_00350"
     CDS             17692..18150
                     /locus_tag="PLK75_00350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY37725.1"
                     /translation="MKKAEIKTEKGVLKVELYEKETPGTVENFVKLAQQGYYNGLRFH
                     RVIPDFVIQGGCPFSKNENDRRVGTGGPGYTIKCETSAPKQFHERGVLSMAHAGRNTG
                     GSQFFICHNRKNTQHLDGNHTCFGKVYEGDEIIDEIEQGDLIESITIIEE"
     gene            complement(18163..18795)
                     /locus_tag="PLK75_00355"
     CDS             complement(18163..18795)
                     /locus_tag="PLK75_00355"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOY37726.1"
                     /translation="MRSVLLTTSYFPSVQYCSKLVHFTDIYLEYYDNYDRQTYRNRCN
                     ILSANGAICLSIPVIKPAVDRQKTGEVLIDYSYNWQRNHFQAIKSAYRKSAYYDYYID
                     ELESFFKIQEKSLFMQNYKILLKLLEIIGIDRNIRITKEYAESNPSFIDLRYTMQPKI
                     QKNSEDPHFFPQPYHQVYPEKFRFMPNLSVLDLIFNEGPLCLQFLEESYK"
     gene            complement(18798..19670)
                     /locus_tag="PLK75_00360"
     CDS             complement(18798..19670)
                     /locus_tag="PLK75_00360"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOY37727.1"
                     /translation="MKRWWIIAVILGMLILVSIAFFKIAIIQHPDVPFSNKSEWVIIR
                     KTAHFPRFYNALLPGEMSIENGDLIAFYDPRVNDTDPKDREILVRRVLGLPGNIVEIK
                     NKVVYINQKPFDEWYPLYFEYRLTTSNRDINELTGKMEVWGIVTITKGKAYNLTTSVE
                     NASAIASAQGVVNIRTLTTPEGEESTNYFPQSDFYNWNKDFFGPVAVPQQHVTHYLNY
                     RNIALSEQIIRFSENNTLEISQHGIKINDTVCEKYTFNKNYYFVMSDDRDHGADSRTF
                     GFVPQDLIIGKIVY"
     gene            complement(19671..21278)
                     /gene="lepB"
                     /locus_tag="PLK75_00365"
     CDS             complement(19671..21278)
                     /gene="lepB"
                     /locus_tag="PLK75_00365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOY37728.1"
                     /translation="MAISKQTKSKSLLGSRTKATKEKRKGLWAKFAKTKTGAFLTNRY
                     FAFGLVTVLFVLWVVWLGNYWWLIGELLIIDIYITRFVRWAFWKPRKDKKMSTLKRKT
                     LEWVDALIFAVVAASFIRIFFFEAFTIPTSSMEKTLLIGDYLFVSKVAYGPRVPNTPI
                     SFPFVHHTLPLTKKTKSFVTWIQNDYRRLAGWGNIERDDIVVFNYPDGDTVVVQNQGQ
                     GYIDIILVKAENMKRRDLAKGKPVLSDKEYYKIARKELWNDPSVDIIVRPVDKRENYI
                     KRCVAIPGDTLEVREGEVYVNRVKQKNIAGKQFKYIVTLKPMEYGNFQGVTGIDANEL
                     KSLGVNYEDMSVVIKTANGYDTENYLKAMENGLAITNQYLIPLTEENVGKVRAMYNVQ
                     SVVKDINEKGNWNIRIFPHSSSYQWNEDWFGPLWIPKKGATMSIDTSNIAPYERVIGY
                     YEHNTLKIDGAKIFINGKESKTYTFKMDYYWMMGDNRHNSADSRFWGFVPEDHVVGKA
                     WVIWFSKDKETGKIRWDRVFDKIHTVD"
     gene            complement(21283..22002)
                     /gene="dapB"
                     /locus_tag="PLK75_00370"
     CDS             complement(21283..22002)
                     /gene="dapB"
                     /locus_tag="PLK75_00370"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOY37729.1"
                     /translation="MKLALIGYGKMGKEVEALALSQGHEVPLIFDANNIASLNADCLR
                     KVDAIIEFTSPQSAPANIALCFETGVPVICGSTGWHDKLPEISELCRAKNACLFYASN
                     FSIGMNIFMEINRQLATIISDFPAYSAHIDETHHIHKLDKPSGTAISIANDIIARHNR
                     YVKWKLNSGDDISDLPVFSFREGEITGDHHVVWQSDIDDISIRHSAKNRKGFALGAIM
                     AAQYCIGKKGIFGMHDLLTKR"
     gene            complement(22056..23009)
                     /gene="fmt"
                     /locus_tag="PLK75_00375"
     CDS             complement(22056..23009)
                     /gene="fmt"
                     /locus_tag="PLK75_00375"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOY37730.1"
                     /translation="MRIVYMGTPEFAVAPLNAIVNAGHDVAAVVTVPDKPAGRGLAMS
                     ESAVKKYSIKNNLCILQPNRLKENDFINQLQNLHADVFVVVAFRMLPTEVWKIPPKGC
                     INLHASLLPQYRGAAPINHAIINGEEVSGVTTFFINENIDTGNIIFKREVRIESCDNA
                     GTLHDKLMSAGSTLLVDTLKAIEQNTVTPIPQSFVILGEKTLKTAPKIFRENCKIHWN
                     MPAEAIYNFIRGLSPYPGAFSHLTDKHGNSKICKIFESTFSIEQHKHYPGTIITDNNN
                     YIQVAVQNGFINLLNIQTEGKKRMNTPDFLRGNSLYGLKFE"
     gene            complement(23020..24264)
                     /locus_tag="PLK75_00380"
     CDS             complement(23020..24264)
                     /locus_tag="PLK75_00380"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOY37731.1"
                     /translation="MSGNQPNDFSEKLNTIEEAIADIRSGKLLIVVDDYDRENEGDFI
                     VAAEAITPEKVNFMAKVGRGLICTPLTEKRCKELDLELMVGKNTSLHETPFTISVDLI
                     GQGCTTGISAADRAKTIAALVNPATKSSDLGRPGHIFPLMARGNGVLQRSGHTEAVID
                     LTRLAGLTPGGALVEIMNDDGTMARLPQLIEIAKKYNIKIVTIQDLITYRLRTESLVE
                     EGVKVSLPTEFGVFNLIPFKQKGTNLEHIALVKGSWRPEEPVLLRMHSSCATGDIFGS
                     LRCDCGPQLHKSMMMIEKEGKGALVYLNQEGRGIGLFNKIAAYKLQEDGYDTVEANLH
                     LGFKADQRDYGVGAQIIRCLGINNIKLITNNPQKRAGLEGYGLQIVENIPLEIEPNKF
                     NLFYLKTKKSKLGHDLHFKGNQ"
     gene            complement(24233..25399)
                     /locus_tag="PLK75_00385"
     CDS             complement(24233..25399)
                     /locus_tag="PLK75_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37732.1"
                     /translation="MIALQVIFWFCVLAVFHSYVLYPLLLRILSSKKRAEAKYSNQTQ
                     PFVSILMSAYNEEDIIEQKVQSVFNTHYPADRIEFWIGSDCSSDNTNSILQIMASKDP
                     RIHFRTFSTRQGKAMIINQLADYANGDILILTDANVLFTPETIPELIKPMTDNKIGLV
                     DSNMVNTNLKKEGISMQEKSYISREVAIKNMESNLWGTMMGPFGGCYGLRKELFVPVP
                     SNLLVDDFFICMHVLRQKYKAVNNIEAVVYEDVSNDLGIEFRRKIRIATGDFQNLRIF
                     AGLLFSGTKGLAFSFLSHKVLRWLGPFFLIIALTCLTVLAFFNTFYLVLACLYAFSFI
                     IPLIDLFFKQNGIHNTYLRFITHFYAMNLALFIGFFKAIIGVKNNVWKPTKRFQ"
     gene            complement(25396..26601)
                     /locus_tag="PLK75_00390"
     CDS             complement(25396..26601)
                     /locus_tag="PLK75_00390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY37733.1"
                     /translation="MKILSLSNKMPYPPKDGGTIGIYNIVDSLGSVGNEVHVLAINTP
                     KHYISHDQIPDEVRKKLHLESVFLKTDISFFGLIRNLLFSRLPYMASRFYSAQYEEKL
                     IDLLQQHNYDIVQFEGLYMCVYIDVVKNNSNALISLRSHNIEHEIWQRTANNEKNYVK
                     RKYLQLMTKRIVELKKRTINKYDLLIPITERDEAVFNRLGNVKPSMIMPACIDIPQFS
                     AGEIGSNPEQLFYIGALDWVPNQEGLLWFINNCWSILKTKLPEVSLHIAGRNASASFL
                     KKVLREGVTYQGEVPDAKEFMKSYGIMIVPLLSGSGMRIKIIEGMALGKPIVTTPIGA
                     EGIPATHRENICIADNEYSFVNEIERLITDKNLCAKISQKAISFIRENYDNIVLAKKL
                     SEFYKTHST"
     gene            complement(26645..28198)
                     /locus_tag="PLK75_00395"
     CDS             complement(26645..28198)
                     /locus_tag="PLK75_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOY37734.1"
                     /translation="MLKRLHLYMLGKFAGPLVLTFLISIFLLLMQFLWKYIDELVGKG
                     LETGVILELLFYASLTLVPLALPLSILLASLMTFGNLGEQYELFAMKSGGISLIRIMY
                     PLVVLTAGIAVGAFFFTNNILPYTNLKMRSLLFSIQQQRPEINLQNGIFTEAVDGFSI
                     KVENKDKNQVLHNIMIYDHRAKNGNKNVTTAESGSIKITSNKKHMILSLTNGTRYEEL
                     EFNPKNKQQSSTYPHQRVSFVQETMYIELDGLGLDRNNEDLFKNGFEMLNIRQLQSSI
                     DSLSNCLLNRKRDIANNLLLYSMFKGKKNSNPESGQTPKITNAPIIVRKFDSLTQRNR
                     SMAGIIKNEQEVKAVNFDSLYNSLSQSQKNKVVDFALNYSRSAETYISNTGDELEYST
                     KWIRRHSIEWHRKFTVALACIILFFVGAPLGAIIRKGGFGTPVVVSVFVFLLYYVVSI
                     TFEKMARELAITPFLGMWFSAFVMLPIGFYLTLKAMNDSLIVSNEQYNRLNNMFKFKR
                     KFKKKGEQS"
     gene            complement(28258..28647)
                     /locus_tag="PLK75_00400"
     CDS             complement(28258..28647)
                     /locus_tag="PLK75_00400"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOY37735.1"
                     /translation="MAENKPKIKIELEYPVNCSRRILFERLSSTAGLSEWFADDVKLM
                     GDVYDFIWDKQSHPAQLIAIKDLKQVRFRWNESPADHYFEFRLVSEELSGSISLVVTD
                     FVTEHEKAEAIHLWDTLIADLKRIIGT"
     gene            28744..28817
                     /locus_tag="PLK75_00405"
     tRNA            28744..28817
                     /locus_tag="PLK75_00405"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28778..28780,aa:Met,seq:cat)
     gene            28921..29793
                     /locus_tag="PLK75_00410"
     CDS             28921..29793
                     /locus_tag="PLK75_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37736.1"
                     /translation="MALYTRYGSLEKEDDDIDIIQTISELKNLLKDVKHIKTGDLKST
                     YIKQITRIAKEFCKQKKVPFKNLCEDSMLVISSESSYFETAIEADLIWRPEYSLELYL
                     NFQLNNYKGKKLNDKRTFVNLISRNVCKRMEKRSPFNVNDRLKIIAKWALDKKNELGE
                     PPSLKMLDFTKDVKLDLFNDIKDYFIEKQRKRLLKLLNGEPIRGKIVFIGLASSLIDV
                     FYRLHNEDCIASSKTNTANWIAQHFRYKTDAIHDFDRDNVYNGMKTKYSNRRPNNSSK
                     IEISFLKPIDSIKR"
     gene            29889..30275
                     /locus_tag="PLK75_00415"
     CDS             29889..30275
                     /locus_tag="PLK75_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY37737.1"
                     /translation="MPEPDSFTKIFNSFEDRLTSIVQEAVFKAMSDYYETHPIPKAEA
                     TLEDDLTDVKGIAAYTKYSVFTIYAYVEQSKIPYYRQPNCRRLIFSKKEVLEWIKGEK
                     NTTKEELVAEARNIKIHVRKNKPSNK"
     gene            30284..30667
                     /locus_tag="PLK75_00420"
     CDS             30284..30667
                     /locus_tag="PLK75_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37738.1"
                     /translation="MKKLIKMKQDPYTGDWFVPRRSNQKFETPENKNAYHNKRKKEQK
                     QEDDLLVNPILKNRNILMELMKDKKIGRFHKQFLLGKGFDLKIIYKMRQEGNNFIYLI
                     SGFELIIDNNHIKIINHASNSNNRH"
     gene            32546..32755
                     /locus_tag="PLK75_00425"
     CDS             32546..32755
                     /locus_tag="PLK75_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOY37739.1"
                     /translation="MNACLREIADLCVISKNLECHLARHNFATTVVLNNDLPIETVSK
                     MLVHTSLNVTCIYAAASTTRKMARI"
     gene            complement(32817..33527)
                     /locus_tag="PLK75_00430"
     CDS             complement(32817..33527)
                     /locus_tag="PLK75_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOY37740.1"
                     /translation="MQLKPIMPFMLTLMVLLSGCNNSKKSQKVVVPAGDSVYGIDISN
                     HQGEINWSKVKDWEGQPIHFIYIKATEGSTYVDKRYEANVKGAKKQGFLVGSYHYFRT
                     TSNPEEQFKNFKRNVAKDAQDLIPLVDVEENKNWNSATFHKNFKKFLVMVEKHYGKKP
                     LIYTVNSFYNKNLSGKYSDYQFLIGRYGENSPNMRDKHDWTIWQFSESGTIEGIPKPV
                     DIDLLNPKFTMETLMLNE"
     gene            complement(33583..34134)
                     /locus_tag="PLK75_00435"
     CDS             complement(33583..34134)
                     /locus_tag="PLK75_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37741.1"
                     /translation="MEEISQPGFRDDDGETYDWLFKYGELYGDILLLNWADFSEKSKS
                     KYAVIYQFCDTIMELCLGFDLRYDVGTKKYYIYPSPIIPAVGVNELNEQCQNANKELF
                     AITKTKNVQNSVYVEWLHIYEKVYDKLYEHFIEKVQLPKDLSIETPDIISFFENYISN
                     AILLKELSKYKKPCTTDDLLKKI"
     gene            complement(34424..34843)
                     /locus_tag="PLK75_00440"
                     /pseudo
     CDS             complement(34424..34843)
                     /locus_tag="PLK75_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(34963..35511)
                     /locus_tag="PLK75_00445"
     CDS             complement(34963..35511)
                     /locus_tag="PLK75_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37742.1"
                     /translation="MEFDYAGYLDRCKTSEDVLFKDAYIAWVKNNGSLSLQNEKFYKN
                     YLTKFAPIPYRVPVELKDEFDWDLLLQLIVSSPITHYIFFHEPSKFPKYYDDLPELEI
                     FYSDDINEKRMVSELWSFQIKHMIEVNLALLIQYQYFNIALEKEKRIIDRQYEERITE
                     YIVKREVAYSKYIRRRDKMFGF"
     gene            complement(35685..36941)
                     /locus_tag="PLK75_00450"
     CDS             complement(35685..36941)
                     /locus_tag="PLK75_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOY37743.1"
                     /translation="MNKKIDDSGYGIALEEYLRHQIYTALQGDVVDDILRIAKIRKNE
                     NYWRNVLEGHSFKVEKQISSKLYALFHEVKNELGFGDSVDFYISGNANVNAFALSGVE
                     ENEPHIININSSLIQLMTDDELRFVIGHEMGHLINKNTELMRLINFIFPEPENAPTLL
                     GNKIRLWNQLSELIADRYGFMAMPDIDVCISAFFKMSSGLDSGRVDLHVEAFIEENNK
                     RLEYFKNSTGLNYASHPINPIRVKALELFSQSEKCNPGKKGALTNEEIETNMDELIDI
                     LLKLRSSEIDVHLTYFIASAGLIIAGCDKDIADKEIDFIIQNLAEFRIFPKSFLEGIA
                     KSGEAMSVFQQSIELILKINPAERERMLQYMIEIVMADNELKNVEVKTIFEIGAKMLG
                     YSVTEVAQIFAGIIQHRFVPSYQSLA"
     assembly_gap    37678..37777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     repeat_region   37850..38770
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=37989..38023
                     /rpt_unit_seq="gtttcagtagccatcttgcaggctaatagctaatc"
     gene            39400..40593
                     /locus_tag="PLK75_00455"
     CDS             39400..40593
                     /locus_tag="PLK75_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOY37744.1"
                     /translation="MEAQQKIFISYSSKDQVQANALCSFLEGSGLNCWIAPRDIVPGN
                     IYGEKIVEAINNCDAVLLFFTENSNRSAHVANEIDIAFNANKRIIVYKLGNFSESPAL
                     MYYLRRTQWIVARNAISDNTLILKTCQNNPCNTPKTLDFSNICNKIATQISQFAKQKN
                     HESETSFTRSFYSFNKKLISFEKNDWVSVVFVIVFIVLLIRIFVPVIVFDHSSIRTWP
                     KLVSFFYHTRNLLTFKAIGILATIVTGILIPPLLILIRKLLKNKFSLVLLGVLMIISL
                     LFAEITPVFLFRNYHLSLGFNDFRYFSLATEMLFLTPLILVIGCGPFVLHYLYRQIEE
                     SNLAFDRKMEYLKTVIHNVTHCENDPERIISELIQEKVNTSGNENEIIRFSVAKIKKQ
                     FEEIK"
     gene            40624..41748
                     /locus_tag="PLK75_00460"
     CDS             40624..41748
                     /locus_tag="PLK75_00460"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOY37745.1"
                     /translation="MHDVFISYSRSDSVEVNSLCHAIEEAGISYWLDTADIPLGTSFP
                     AEISRAIDSSKLLVFVSSVHSNKSPYVTSEVRYALESGKMIIPFKIDSENYSDDLKLF
                     LVTTNYFAAYPPPVSKYMTEFVDRLKLLIDSTDNNRPVLKRIIDENDPDVPALLEIYH
                     NCFENDRCVADEFILSNLFADNNTNHKAYLFVLKKMNRNIGMADVSYFCDSKLLFVSY
                     IAVHHFRYPGEKLIYTHNIIEGIQNYFQQQHLEIDALLFETEEERIYKFFNRVFNNRF
                     HKKIYKICIDYTQPTLVSDSDPGVAGGNKIHLVYMPCQDSGERTSIKKETVITLIKQV
                     YFEIYLHIRDNTYEERYKYLDELIREYCNHLPEDILLEWE"
     gene            41987..42985
                     /locus_tag="PLK75_00465"
     CDS             41987..42985
                     /locus_tag="PLK75_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY37746.1"
                     /translation="MRFKLYFFSIILSGFLASYNCMDLYGQNAQEFVTCIVLDKTASM
                     VGKPDGSNIWADVQNYTLNMIDAISINTTVIVFEFDGKCYGPKTFTISQQADKGAIKT
                     YVKSVKPDGQSTAIYNALDVAWQYMQNNYSNHNKIIYLITDGRDNASQMSFSSVLSKF
                     SANKGEYDHLYYIDLRDMADANIKNLVSQTESVSITKGFVKTITVQPIFQEIPVTLDK
                     SNKIEQRFAISGGVLPESFSFNCQVVSPNNINADINPSGNIGQQNLTKIDDGKYSFQF
                     GLDILQGQLVDSAEFKISLHSNSINEYLITFQPETFTILLKNKDVAKVKAKNGGWR"
     gene            43015..43902
                     /locus_tag="PLK75_00470"
     CDS             43015..43902
                     /locus_tag="PLK75_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37747.1"
                     /translation="MKKLNLLLFMVISFGGFANNAIQCTDSITRQIELEFNKEAIEND
                     AFITWELIGNWQEFTITINQGVLEENSFTVKAKDYKDFAKGFDGIRICIKPNKHTKEG
                     TYQLSMKVSDISENLEFSKEELDLIIPEFYYQAPPPRPLWQYLVFGLLIAFLLFNIIW
                     FALVKPNKYRKMNGTLIFDDGAAIKLNNCYKYCIYTAMTAPKLTKNGFLSNLYFGKVG
                     KYGIINFPDYLRNENTNIIIKQVKDRNKWKNAVISHTNLQLISEYKKLYHEQDYVFQD
                     SINPKAQISFTYDNIKHQL"
     gene            43933..46878
                     /locus_tag="PLK75_00475"
     CDS             43933..46878
                     /locus_tag="PLK75_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HOY37748.1"
                     /translation="MKTKLSRTVFIGLGGTGAQTIVRLKANFMENFGKIPPMIKLLAI
                     DTDSRLKISYSTTSGKEYMLDEHEFKHLMVEDVVSYINNNPKTCGFFPKDLIKAKTSI
                     YKGAGQFRAFGRLPLIREFDTNFKVFLENTIKNVRNWIGDRDSDFEVDKTGTNVFVVF
                     SLAGGTGAGTFIDFPLMIKGSGILETKEKIIGVGVLPDVYSSFGLHAINTKPNAIAGI
                     TEYEFLANNVMGEMLINPNSPELLINTPTAKYKVDYNYLYDSFILVNNYSTTNQQYDK
                     PGEIADLISKSLFLVAGSAGGENESTLDNIFTNAKVSEESYGKPSRYLGIGCAELVLN
                     RERIADYCSFLLAGQLCQLISREIGKQDYDHAVQDRVSGWNIQEDQNQDDVITKLLSN
                     KLKNRFINLSEFDDGAAQTIKAKRTAWIESFTVSIKDEIYSDEGSLNQLKGDKLKNLT
                     DYIYSKVSEPGGVAFSNNFILVLKGRLLAMIEEMRTESAAFDKKKEQVIRGYDEKLAA
                     ISASEKISKFNVFKNKSRKIEEACEDFVESVNREALYISEIERRNAAVSFFQSLIDRI
                     NSMQNQIVNFQSRLSKIITIISKNIQTIRSRNPQEPFTVHLTPHIIQNIKVNANEDDL
                     ERFIKNNLPASFIFTEQTEIELLNIFLKFCQKEHITQENKKLNILDALNELEKSKVEE
                     ILDDLKDAIGILRTIDKNYDKITVANKYVIGVSNSNHISLINHKNSQESFEKKTEVAS
                     KTEIQTIEGSTVSKSIPDDLGHILDRKFSENGKAPMFANTGDESKIIITAFESTVPAF
                     VVNNFRNYKQEMDNSTGISPIFRFANKYWGDIILKTNYSIFPVDTDDTLKIWSLGLLV
                     SYVEKMQLIGKQKNRDYFVAVRSMGSNITELDSFRPKAFEIFNQKQLGLELMVEIKDK
                     IRNNLSTYKSLINELIEDKTDKKYIDEIANHQLDKKTLNSNSHNQTRELILKELDFIK
                     SIGNLSEII"
     gene            46925..50674
                     /locus_tag="PLK75_00480"
     CDS             46925..50674
                     /locus_tag="PLK75_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOY37749.1"
                     /translation="MGEELISQFRRSILTDFQREYSTKLQTKIETLFIESAGIVNEQK
                     QTLFDLSELKDESATEVVWNKLISEETKILNVISDAIDSISNVRDDLALNEVQLTEIG
                     ESINIIVLLPTYKTRESVWLSYLLKVIFPQLGITNYNYFVFGVLPELDKDYKSNENEY
                     LCRCFACLTECNEILNEKMDLINSFTLLSDKNEDNTAVENYSELIPLLKELFLFVFYN
                     ESPFNHNRQSPIPVYREKRAFFSALGLSAVVYKRDRLLEQFVHNIIVKVFEQFLEINR
                     KPINREGFYIEADQLIKNLAWEKKESLFEGPEAYQSFSNTYNLSANSISNLSTDLMLK
                     SLNANKEEHESYNGYYGGTYLNRVLLEKDKSLNKTKEKIVETIANYLKEGSQGFSKAL
                     ALLFLLMGKEDDSVFEGNSHVEPADLLYIKAKVAEYYCSFLTESELIPILDFKNKEKE
                     LKRKIEQAEKLRCKIENLENNIYNFQEVKEDLSKQTGELLYFTISGQKVNITGIKKEE
                     EPIPDTMKAYIPEITDKPLPSIVDLRPYLTPVENQLQVGSCVANACVGAYEYLVKRAT
                     SKNKDFSRLFAYYNARNTDGTGNVTDEGAIQSNCIKSMMELGVCLESTWGYNVDKVNT
                     RPDVKCYEEATNYRVIDAEYFGIDIDLMKKCLSEGFPIIIGLNLYNSFQAVGNKGIVP
                     LPDETEEQAENHGRHAMLCVGYSDADKYFIVRNSWGTGWGDAGYCYIPYEYMGNSNFH
                     TGSIVFLVKALTDLDFKKIDFTKNIAKEKGSLFNFSYDLLELQHSKEKYFAIKQELEN
                     LVPEFENLQRRLIDQRELVLDYQFREEVELNQIKELGKSQEEIISEILNKDQLLNTLN
                     DRKSELKQKRKRFILRRFIVVPIVIVLFAVLAYVVVGKFLSGVWLISFALILPILLIS
                     VLSVFFIVYGIVNYIALKRKIINELNKLQEQILQEEEIRRSFIEKLINNYNQYFEVKR
                     TNKLFELLLDFISDVNDYLNISKDKLLQWLVILKQQKEKSEKFLTDNEITDNGATILQ
                     YACTTNDILNLVNRVDTLKEFYNDGKRPLHEFVLDNNGRIMNDEGIDIFKGAAISFAQ
                     GKTEYELMKGFNILDFVFKSEFISKVFKTIDMTHDLKTNVKMYALYSKPFIKVTSKYD
                     TISIDHSIELLWPDIEDHSFDKIRDIVESIYAIKPKIFKYSNSTESALIFIQILTFFP
                     AYAVESLEKAARVFLKLPPAERNKYFIKPKFGAQSYMPELDNELH"
     gene            50698..51075
                     /locus_tag="PLK75_00485"
     CDS             50698..51075
                     /locus_tag="PLK75_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37750.1"
                     /translation="MSNLVKIVMIIIVAFGFSSCTNSTKTKKIEKFHAFIEEVGQNHE
                     SYTREDWTKADSTCKEFTDYFDQPEIKKILTQEEAEEIAKLKGNYKGYRFKPKLDETI
                     DDVIDKASEVGKELEGVIETIKK"
     gene            51086..51589
                     /locus_tag="PLK75_00490"
     CDS             51086..51589
                     /locus_tag="PLK75_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37751.1"
                     /translation="MDNIFTFSNFLESIEKKNPELYAHLMGKYTKKIRLTDIPEIKEL
                     VEEFDCTNLYFGSLPISIDDTLLLVRLIAASISCDYELSYDNEVHHELLTITVGLGVE
                     TVNSELSELLKSQLEQLFIILLSQQLELADEIKLERRNRLADWEIKRAMNTSKRYYNV
                     FFERNYD"
     gene            51657..53072
                     /locus_tag="PLK75_00495"
     CDS             51657..53072
                     /locus_tag="PLK75_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY37752.1"
                     /translation="MRLYYGLKYRYDNINELRFSELARLLFYSMSNSVTDVQRFISKL
                     NKFDPANLETYCSGLDLEVYTIKENLRYSAHFTNRMVERLQNWIDNIGCTRQEIEDFI
                     SYYKIRSDKLVGLRMISKSQYNSPADINTMLGQYIIGQERAQKILSSAIYIHLLRCGL
                     VKPLISAGYTTQNSGLVPLLPNPNIVLIGSTGTGKTYILKTIASVFRLPFLKVDCASL
                     TSTGYYGNGLNEHLYTFLNKCQESKQDINQAIIFFDEFDKLSEMHYARSGGSLGGVEM
                     QQEFLNIIEDKEILLYPPKGSQNEGKSLNLSNCMFVFGGSFYGIERIVEKRLGKHQRI
                     GFKMHEKNTNDDAPVLSHVNPHDLVEFGILPELVGRINHVVSLEEHNLNTIISIIKNS
                     AESPLKAYENYFEMHHDTLVIEEDVYELIAEKILEANTGARAIYGVLNELLKDLLYHS
                     PNLIEEKLVVDKAYFNKVFPK"
     gene            53239..54621
                     /locus_tag="PLK75_00500"
     CDS             53239..54621
                     /locus_tag="PLK75_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37753.1"
                     /translation="MNPNEIKIIDDSGVEQLIDFELRQCSQIVPFREVKAQIKKMFLD
                     CYNHVETLFGDHAPRLITVRLEQEKTNLQSVIMASFRPTISTVMELKFSFHYHTVQLV
                     CADLNDKTDENTGKIKRIILHELIHAADLKNLNQFHLSHTAKSAQPGNIPSQMIFGET
                     FGSGQYNYTSHYLMLHFIQKFRAEGVAILGERLLNQHKYSQEIGEFIKYFASDFHYVI
                     GKSRDIQFFEKNIDKVTVENLNIICERAYMYGDAVMFSLIAQKHGKDFKFLDLSRGFD
                     NFIIPVFTYSDAIEFMKLCINFDMSDYINGMLRLTDNNGNTLIDHNKFYNYCGFIQNE
                     TTGSINEFCLNISKTALHYNKEAFISTLKDVLGSKMQKEEIIELIAEFENTTPADNIS
                     KDIKVLASQLVEKINTEDSEIVIWALTYLLDDEDMIHDELDFVGLQDDWFVLKAAAYI
                     ISGNEKSNQL"
     gene            complement(54612..54755)
                     /locus_tag="PLK75_00505"
     CDS             complement(54612..54755)
                     /locus_tag="PLK75_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37754.1"
                     /translation="MDKSGIIVKIEALIHTYDPNTIEGKKRIEALLEYKRFVEMPFAT
                     TYN"
     gene            complement(55432..57093)
                     /locus_tag="PLK75_00510"
     CDS             complement(55432..57093)
                     /locus_tag="PLK75_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37755.1"
                     /translation="MSKIKITPAQISRLFNKFLLLDDTLRDNLRKYYEYREGNPLNQS
                     GNAAISSEILNLTHVKQTYNTDQYFRRVCYYVNLNLLSGITIQPEPDDRDITRLLKLL
                     ALDFIRLYKDQPLTNTVKLLSETLTDKNSNHIALQEPQPIKKQTIALTPLFYWQKPES
                     FIGRKRYLDTLSLHIDSNKYTIINGIKGIGKTSLVQYYVKLKASNFDHMMWINVTKSI
                     KLSLIEAMSRSVAIPQFIAPEINNEAELYQCFTSASEILLQYSGNNLMIIDNAESAAQ
                     ILDFIGSFEGLNIAWKVIFISSSRIATPIPVRLEKPDPGDAYDMLKSYYLEDIDAGEK
                     QQALQFIESNYQGIHTLLKNLSFNPLLIELIARVDIADELLDLCDIYQIIKGVSSIKH
                     NRLQKPVITRTKDNLPVALSPEECTPMCYIKALFCRDLIHLNDDQKKMLMVFAVLPSQ
                     YIATEDLKLISPLGNNRITDLLHSIKGWLKFDRDTSSFYMNELYQFAIREILQPDKTF
                     TNSIIVKIDALIHTCDPNTIEGKKRIEALLEYKRFVEILTCPTIN"
     gene            57193..58191
                     /locus_tag="PLK75_00515"
     CDS             57193..58191
                     /locus_tag="PLK75_00515"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOY37756.1"
                     /translation="MKTSARTHNFTMFVLISGTLVLSGCCKQKSTPENYKPPIFSENG
                     RLYMNANPEVDMFSVRADSVFGLYSNDSIGLFIKDKRYGYLNVNNSGVVIPAKYDLAW
                     QFNDSGSVAAVVAGGKMGFIGRNGNYLVEPRFDFDTAYFNDYDFTYSDDYCYIPGQKG
                     KFGIINRRFQVVVSPIYDNIEIDGNYFIIWDAGKAGIADTSGNIILKPEFDNIDVIRM
                     GFVIYNEPKNEKYVLDFDGITTVASGVFDFVYSIRSSRSRSDEYYDDENIYVADSIPV
                     FYSLNYAKFEISSLQGVIHRQSGKIVCPAKWDDVFMLSEDVLVGVCDDYYFIIDIK"
     gene            58203..59705
                     /locus_tag="PLK75_00520"
     CDS             58203..59705
                     /locus_tag="PLK75_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY37757.1"
                     /translation="MEKRTKYFGLEYPTQIHLPEDVKYTLAKLLFASPTAGIEEVKLV
                     LENEKLVSAVENLIFALDFEIVSVGQPDEDGLEFMSRKETEKLMKKKIKKWISHFGSP
                     TENEILDLANDKKIHNKEFWLEVYEKMKRVKINTIPEIESYLKQYVKGQDEAVRTIAM
                     MVFEHRLRLSEHIDLPNNAVCMIGSTGTGKTWLASKAGELLNVKPYRINCAELVPSGI
                     VGMSLEKHLTQMYMLYNGNNKKMSRSMIHFDEIDKISYRYHISDAFKATLQLEMLRFF
                     NKGEKLNFNTRSYYLSESTNIETGSMLLVFSGAFNGIEEIITQRLIQENDGKLFLVDT
                     DNMIQYCKTDDLVNYGFIPELAARLNNICPLQPLTTTGIREILSNAQESELSRHMNKC
                     KALNLKVRFSDDAIALMAENIVKKKIGARGIAEVLTPLLSHYYFNADQYKGKEIVIDG
                     NAVRRINFHRNYKTLAKAFEQKTPLNEIMEQFKASADEVLDLYLKWKEIN"
     gene            59725..60783
                     /locus_tag="PLK75_00525"
     CDS             59725..60783
                     /locus_tag="PLK75_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37758.1"
                     /translation="MLRFDYYDYYRTLSEYEYSEGREIFEIWKQAGGSFDPANDKVMD
                     YLNCFTPIPFRQPSFDFDQKTIDLLFKLVVASTSCTYLFDLKESKIPELVIFNTNICL
                     GNESFKLSECTEDIIEVLIFVLLHEIALILASKYDDVMEDKIKQYKEKCMKLLLPKYT
                     NSNNQAIQLKRGFQFNYSEFIESIERSDNADNVKLYRMWIANHGSKNIEKEKFHSYLN
                     EFEYFPFSVPPDLISSFDWELFLKLVAGSTSISYDFVFEPSIARNETEVYPELYLYLE
                     GESENICKKVSELEPNQVFRLFEIYCMEFIQFQYMGSLFMPNSTLYRNDFKKRIELYK
                     QKRNEAQMIADRRSCFFE"
     gene            complement(60818..61627)
                     /locus_tag="PLK75_00530"
     CDS             complement(60818..61627)
                     /locus_tag="PLK75_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY37759.1"
                     /translation="MKTYNVLGVNDELIGKCSLQQKKILNEYFLQIQSKLDYPIKNEF
                     FEILDSKTKEKSGFIKPRDLVHRDGNWHGAIHFHIYCKENNKTSLVFQIRNSNKDTFP
                     RMIDTAAAGHYLPGEELQDGLREVEEEIGLKIELQNVINLGKHRFYFFQEEKNIKNCE
                     VQDVLLFQSNFPINKYKLQKEELHGIIKINTDDIIRLFKMECNVIDCEEAYIFDETDN
                     LVKRELSITRNDFVPCIYDHYFLKTAFITKMISNNMHVVENPYDYDVLTYF"
     gene            complement(61851..62018)
                     /locus_tag="PLK75_00535"
     CDS             complement(61851..62018)
                     /locus_tag="PLK75_00535"
                     /inference="COORDINATES: protein motif:HMM:NF020006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HOY37760.1"
                     /translation="MKEYKVITHYVTPEFEQLVAHALAEGWQLVGGVSLNLVEKGDGE
                     YQMIFAQALAR"
     gene            62221..63558
                     /locus_tag="PLK75_00540"
     CDS             62221..63558
                     /locus_tag="PLK75_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37761.1"
                     /translation="MLHNELISHRRYTDFLKCSLKLLPQNGMPQAKIATHVFEAARAE
                     RRFLKTLFNTLNSQTVCEYVPMYKDIQQRHAQTIDAIKRGCDYIFNAFFVCDNKVASI
                     NLLRKVAEECSNSVCYQVVLVTEADEITRDHLVELHWNYSIASDFITQPLQSAGFLNR
                     RLSLQLVDLGESLGFYNKLITDADASFSAYENNNNLICTELSPWCYSCIKRYICIPTL
                     IKQRPPSILSAVTPSRSRFLRENNIHNIDMLNALSDDALMALNFDVFDIRNIREQCQR
                     ISERKPVFRVHLKKDYFGNVVFVSLQLARQNEVLFPRKILFSHGAEAFEITLHYDADT
                     KSIGVDADEVDRFNSLFKEKIVTHGLDALWLKRLSTAHGIYNTHIIDLADFVAQFVHM
                     PLLGLELNELKYLLNPGDMVLHSIGNIRGAILSPYERLILVKELVNYFVKYMY"
BASE COUNT        19760 a        11783 c        12403 g        19540 t
ORIGIN      
        1 tagtaaaata ttttttttca taaactcaat cttccatatt taatgcacca caacgagtga
       61 ataaccgtaa ctgtggtgga tattttttaa ttagacatag ggttgataat tcactaaatt
      121 gttgaaaatc aataataaat ccgtttgtaa gcaatttgtc ccgtttttcc atgatttttt
      181 tgcccaaatt ttcggtgaac tccattttca aaactacgaa tgttaatttc aatatccaaa
      241 ttattcatta accattttat tattgcagct ttaaaaattg gtaattctcc tgagaaccac
      301 tgcaacggac tgttgtaaaa aaaatgtgaa atcaacaaca aatgactgtc agttagcttg
      361 ttgtatgccc tgctttagtt ttcgaaaacc tgctcaaatt gattcttgta cccgatatac
      421 aatgggtcgg attttccccg tacctgaccg acgaaattga tatatccttc gagccgctga
      481 agaaattttg cctcgtgagc ggctttcact ttgccggtta ttttaaagtg tttttgggtt
      541 gcggtttcaa caccattggt gctcaggtca tgaagcattg cgcggatttt ctttagcatc
      601 tttcggttta catttacttt ttcgttcact gtaagccctg taactatctg cctccggttt
      661 gccggtcgta ttctcagttt gttgctgtta atttcgaaat tgtttttttg aatcaaacta
      721 atgatatcaa gcaaggtatt tgtttcgatt gcagcgttcg atgaaaacgt taaatcatcg
      781 gcatagcgcg tatactccag attgtgggtt gcgcaaaacg ccttgaggtc ggcatccaga
      841 ttcaaacaga tgaaattaga aattacgggt gagctcggtg cacctgccgg tagccttcct
      901 tcgtaagtgc aaagcagcgt gattgcggtt gcaacttgat cgctatatcc aaaaacggga
      961 gaggagaaca agtcaagaac ctgctttgcc gtaatacgcg gaaaaaaatc cttcaggtca
     1021 atattcagta aatgtttctt gcccgtgtgc gctttggcat tttcaacaat attgcaatgt
     1081 gatcccaagt agtgcgggtt tacaacaaag ccatggactt cttctggttt aatcatcaga
     1141 taatacgcct gaagaaaata attcaaccgc ttctgaatgg cttttagttt tttattggga
     1201 gcatatatat gtctgttgcc accacgtttt ttcttaatgg tatagtgtgt gtattgcggg
     1261 ttattaataa tggattccaa ttgaagtaac cctgatttaa aaaaactgca caaatcaatc
     1321 ggtgtttcta tcgaaagcag atacagcgca ttcaactgat gcttctcaag tatctccagt
     1381 gcaaccggca atctcaatga accatcggca aggtggtttt ggagtacacc tttatttctt
     1441 ctggcgaatt caattatggt tgatgtgttg gtcatgctta aaaaaaattg agccgttgca
     1501 aattatcttt tgactcgctg actatttacg agggcgaacg aagtgaacaa cggagtgaga
     1561 ccacgtaaat agtcgaagtt cattccttcc acactgtaag ggttgcagga acacaaccac
     1621 catgtcgtac tttcttttga catcataatt ttttaaaaca acggctcaat aatgtgtatt
     1681 tttaaaatgg tgaatcaaaa ctggaatctg aaccggactc atcacaggat ttactgtcgg
     1741 gggatagcaa gctgcgtaat ctgtttttcg aaaactcgaa attgttttta tagccggtaa
     1801 aattcataaa ccgcgtaaag tctttatcta tcataagttg aatcgaccct gtgccgccat
     1861 tacggttttt tgccatgatt atttctgcaa tcccttctgt gcagttgcct tctctgtctt
     1921 ccaacatgcc ataatactca ggcctgtaaa taaatattac cttatcggca tcctgctcaa
     1981 tggctccact ctctctcaga tccgacaaat gtggtttttt ttcgccggtt ctcacttcaa
     2041 cagcacggct catctgactg gatgcaatta cgcacacatt cagttctctg gcaatgtttt
     2101 ttaattcccg gctgatataa ctgatttcga cctcgcggtt attcttgtat tttgaagaac
     2161 tcatcagttg caaataatca agtataatca ctttaacacc attttcacgg atgtgttttt
     2221 cacacatttt tttaagctgg gtcaccgagt tgctgcaact gtcgtttata aaaatgttac
     2281 acgctgcgat tgtttttccg gctgctgtca attttccttt ctgctcagcg ctcaaatcct
     2341 gctgtataat atttcccgct gctatccccg aaactgcagc aagaaaacgg ttaaccagag
     2401 tgggttttgg cagatcgaag gtgaaatata acacgggatg ctcttgcgcc atattcagcg
     2461 aaagattaat aagcaactgc gttttcccca tcgacggtct gcctccgata attaccaatt
     2521 ctccctgcga aaatcctccg aacgtaatat caaaatcacg gaaaccgctt ttaataatgt
     2581 tataatcctt tacgccccgc agcaaccggg taaggttttc ttcaacaagc cctgagatgt
     2641 tttccgattc tctgacgact atattttgaa actctttctc ataaatcagt tgcttaagtc
     2701 gcaacaaaat ttcacgctca ttgctcgttg gggaggcaat gattttttcg atttggtgtt
     2761 ttaaattttc ttccatggtg taaaattaat gcaataccac agtcgattct cggttgttga
     2821 aaactatttt ttagaatatt atttaatagt tcagaaagcc tcccgtggaa atgtgtatta
     2881 aagaacgata aaaagagatg gtgaaattat tccttaataa acttatatat cgtattcgag
     2941 cctttcccca gtttaagata atataatccc ggtactacac cggaaagctc aattgttgtt
     3001 tttgagttta gcttcccgga taaaatactg cgcccggaaa cgtcgttgat ggcgaatgat
     3061 atttcatcgg agggttctgg aattaaaaca tagagaatgt cgctggcagg attgggataa
     3121 agagttacac cccctgaatg ggttcctgtg aatatactgt ttgggtcgca gaacaccacc
     3181 tccatcacta cccccggtcc ctgaatatag tcgggacact catctggagc cccctcaaca
     3241 taatagagcc ccggagaatt taccgtaagt tgttgagaat gctctccctg tatcatttca
     3301 ccgtttttgt accaaataat attaattgta tatgggttgt taagggtgaa aacaacttca
     3361 tcgccttcgc atataatggt ttctccgttg gtgccaatag taaactcacc agtggtttca
     3421 atggtaggag gtaaaaaagt atagccgtcc acaaagaatt cttccgacaa ataactctct
     3481 ccgttataac ctacttctac cgatagatag caggctgcaa aattataata gtcaatcgtc
     3541 agattctggt taacagcacc ttcaatgggt aaggtctccg aagagccata atatcttcgg
     3601 taccactgat atgtctcata aattccggtg ctcacttctc cgctgctttc agggcacagc
     3661 atagtattac ccgtaatttc agggggtatt tccagaacat aaggttgcac attggtaatt
     3721 tcatagattt taccattagc gtatcgtggg gtatatattc ttatggtatc gtcgtttctg
     3781 ccatcgccgg catcaaccag tgctgttgcg cctgtggccg catcaatagt actgaaattg
     3841 tacatctgag tctgctcatt aaagcggtat tctctgaaag ctgaatcaac atcggtgctg
     3901 taaacataca gtttacaatc gcttttgggt cttgctccga aaagaaaact gcgtccgaac
     3961 cgaccgctta tcttgttttg aagccataca gttccgttcc atgtatattc ataaatacca
     4021 ctgttatcct gcgtgagaac gcggttctgg tggtcgtttc gcaaatcggt tacgattacc
     4081 gtttctggcc agccatcggc ggccgatatt gatgttgaat cggtgtatcc ggctccggtc
     4141 catgtgtatt cctttacatg gtttcccgaa acgtaatatc tgacctgacc atcgtttcgt
     4201 cctttggcaa ttgttgtagg tccttctgtt ccatgcccta aacttacatt atgaatattc
     4261 catccgcttc cgttccaggt atattcccga tgaataatcc acccatctac gtaaacacgg
     4321 gttgtgccat cgcctcttgc atcaccaatg gtaaccgata gcatgggctt ggctgtggtt
     4381 tgtatcagcg acgaaaccca ggcagttccg ttccatgaat attcaaatac cctgctggtg
     4441 gtgccgcccc attccacagc atacaggcgg ttgacattat cgtttcgccc ttcaccaaca
     4501 gcaattgtaa tcaggttttt aagccctgtg gctatggtac ttgtcataac ccaggtcgtt
     4561 cctgaccatg tccattcata aattgctgca tcggaaggct caccacgcgt ggtcacataa
     4621 atacggttga taccatcgtt tctgccattg ccaacgcaaa tgccataggt tttatcgttg
     4681 ggacaggttc ctacttcgcg cactatccac tgctggcaaa aaaccggaga agccaaaaac
     4741 aataaaaaaa gagcgaaaat gttgtttacc atttatttac atatatttgc gcaaatttaa
     4801 tttttcgttt atgactttaa ttaaaacagg aaaatatttc ttcatttttc cgggtttttt
     4861 tctaatatta ttttactcac tgggttgcga aagtacgtac attcgaatgg gggatgaagg
     4921 gactcatact gcaccagctg atacagttaa gctctcaact catgtacaac cggttttata
     4981 caacaagtgc gcttcatgcc attttgccgg aaccacgctt gaccttgaat ctgccgacat
     5041 ttacaatagt cttataagcg gaaattttgt cgataccgct tcacccgaaa acagccggct
     5101 tttttcagaa cccgatcccg ggcatgctga cgattatctt acggttgatg aacatacgct
     5161 aatagtgcgt tggattcaac agggagctaa aaacaattaa cctatatggt aaaaaatatc
     5221 ctttcaaaat ttgcaataat tgtaggtatt gtgctgtatt gcgggttggt tatggggcaa
     5281 accgatacac ttccgaatag cgcaaagatt cacaaacctg taattaatcc atggtcagga
     5341 tcattgatca ttaataatca aaccactgaa ttgtctccca aaaatggcct ggattttgta
     5401 attcatcacc ggtttacgtc ggtagaaaac ggattgagcg atttattcgg gctctacagt
     5461 gcgtcgaaca tcaggttggg gttgcaatac agtattacga acaattttat ggttggattc
     5521 ggttctgaaa aggataacaa atgtcaggag tttttcctga aataccggtt tcttacgcaa
     5581 acgcgcgacg gcgttatccc tgtttcggtt gctgcctatg caagtgctgc tatcaatgcc
     5641 cgcgagaaag cgtattttgg cactgattac cgatttgcca accggatgtc gtattttact
     5701 gaactgttga tttcaagaaa atggtatgaa cggctttcaa ctcagttgtc ggttagctat
     5761 gggcacatta ataaagtgga aagctaccgt gtggtatcgg gcgataccat atctgaaacc
     5821 gttacatatt acccaaaatt ctacaacgat gcactcgggt tgggtattgc agcccgcttt
     5881 aaagtgatag gctcatggac acttgtgggt gagtttgaat accccatggc attgggaaaa
     5941 ggtggcagac acggaaatag cagcattccc cgtaaactta aaccaaaacc tgacgcagct
     6001 ctgggcttcg aagtgagtac gatgacccat gtttttcaac tttttgtgtc ttcatacagg
     6061 gcaattgttc cacaatacaa cttgattacc aatacttacg attttaccga gaaaagtggt
     6121 attatgcccg gatttaatat tattgtcaaa ttttgaattt gttttatgaa aaataaaaat
     6181 ctatcgaagt ttttaatagt aactgtttta tcggcaggat tgatgtcatt cctagccgtg
     6241 gagttggtca aagacccatg gaatgttcct gcacaatata aaaccatgaa aaatccggtg
     6301 aaaaaagaag caaccagcat tgctgatggt aaagcactgt ttgaagctac ttgcgccacc
     6361 tgccacggga aaacaggtaa agctgacggg gtgaagtcga aaaaccttac aattgaaatg
     6421 ttcgatttgt cggcagccga ttaccaatcg aaattctctg atggagaaat atattatcag
     6481 tcttttattg gccgctatcg ctggcacgat tttacaaaag taattccgga tgaatcggat
     6541 cgctggtcgg tagtgaatta tattcgaagt ctgaaaaagt aattttttaa tttttttttt
     6601 ctgatgattt ttccttgctg atacttttat taatgcagaa cagggcgctc cagaccggga
     6661 ttgaataatt tgaaaacaat tatgaccata aaacaattca ataaatgaaa aaaaaggtat
     6721 tactactttt agctctagcg gcatcattcg gtgctggttt cgcagttaac gcgatattta
     6781 cgcaaccaac tgaaaaacaa atacaaatga aacgtgtaac cggaatcggg ggcatttttt
     6841 ttaagtgcaa agaacccgaa aaacttaaac aatggtatca gactcacctt ggatttgaca
     6901 ttaatgaatt tggggcaatg tttgcgtggc gtgaggatgt cgattcatcg aaaacaggat
     6961 ataccatctg gagtcctttt aacgagagca ccgattattt tgaaccttcc accaaggact
     7021 ttatgattaa ctatcgtgtc gaaaaccttg aagcactggt aaaagagcta aatagggaag
     7081 gtgttacagt actcgatagc atcgaaacct acgattttgg caagtttgtt catattctcg
     7141 acctagaagg aaacaaaatc gaattgtggg agcccaccaa ttaaacccag atgttatcgt
     7201 ccgttttttg taatttcttc ttcgcgcttt ccctgctgtc gtaacttccg aaggggtttc
     7261 ttttttacca taccaccttt ggcatcgaaa atagggttca taattacgaa tgcttttata
     7321 tcgattttat cgatttcgtt tttcagtttc gaaagctcaa gacgggtaat tactgtgtac
     7381 acaacttccg tatctttcag caattcgcca cgcggagcaa aaccattctt tgccatatag
     7441 agagtgcagc cccttcccag attttcgata atggcaagcc tcacttcttc gggtttgtcg
     7501 gtaattatcg aaaccccgat atattcttca ataccatcaa caatgaaatc aacggtcttc
     7561 gaagctgcaa aatacgtgag aatggcgtac agtgctgttt ctacagaaag tatataagcg
     7621 gctgaaagaa aaatgaatac attgaaaatg agtattaccg tacccaccgt aagggagaat
     7681 tttttattca gatacacagc catcacttcg gttccatcca acacagcccc gcctctgatg
     7741 gcaagtccga ttcccagacc taagaagaat cctccgaaag cagcaaccag aagtttgtcg
     7801 tgagtaacca cagggtaggg gataaaatgc acggctattg caagcagtgt aatggctatg
     7861 atgcttttca ccgcaaattg tctgcctaca gtcgacaacg ccagtaacat gaaaggaatg
     7921 tttaatatca gtatcagtaa cgataccgaa atactgctta cgttgctgat gataagcgaa
     7981 ataccggtta taccgccgtc gataaatgag ctgggcatca ggaacccttt aagtccgaag
     8041 gcagctacaa gcactcctgc agcaataaat gcgcagttga taagaaaatg ctttgcatcg
     8101 atttggcgtc gctgcaactc ggcaatatgt tcgtgtttac cgggttgcct gcctttacgg
     8161 atgtattccc gttgcaacat gcctgccagt aaccgttgaa acaatgaact catcttgcgc
     8221 tgctatttga tgggttttac cgaatttgca atggaataca gtttttgaat atcattgaaa
     8281 ccgtattcgt gaaattcgtc agaaataatg gcaaacggac tgttggcacc tgtaacgaca
     8341 caaaccagat aaaaacagct gtcattttta tcgtgcgttg ccttatatac aacctgactg
     8401 tcgttttcaa gcacgtaccg gtcaaattct ttaaaaatat cattttctgc atcttcgcgg
     8461 tattgtgcaa gtgtattttt actgttgcgt atattcattc tcacgtcgga agctgcattt
     8521 aactgtatca ctatctgatc gctggtgccc tcatcggtaa taagcttaga agtgtagacg
     8581 tctgcatcgg gtggcaccat cacagtgagc ggaaacccat aatccgaaag gtctttatga
     8641 acgaaatcag ccggggtttc acgggtaagc acaaactctg aatcattttt gttactgttg
     8701 ctttcgctgg ttttgtcttt tttatttaca ccacacgaaa tcagtattgc ggaaatgaca
     8761 attactaaca tgaaaaatga atatttcatt tgcatatatt ttatgcgtta aaactactaa
     8821 ataacaggtt atgttgaaag ataaattcga agttaattgc caaattatta ataatggttt
     8881 gttacttttg taaggaaaag ttcaatatat gacacgtatc ggtcgccaga tgcctcattt
     8941 tgaggaaatt gatctgaata gtatatctgc agaattttct gccgaccgaa aatacaggta
     9001 cgtgcttacg gcgcattacc gcaattcgct gttgcgaagc gatgccaatg aacagattgc
     9061 ggttgtgctg aagaatccga gttctgccga tgcattcaaa tcagacgcta ccatccgtaa
     9121 ggtagaaaca tttgtacaca aacatttcga aaaagctaaa acgctggtta ttcttaatat
     9181 ttttgcattg cgtgccaccg atgcatccga tgttcagcaa tgttttctgc aatatggcta
     9241 tgattatatc accggtgatg aaaacaacca atacattgca aaagtgctga agcaatccga
     9301 tgcaataatt tgtgcatggg gaggcaacag cggtatcgat aaaacagtgt acaataagcg
     9361 tattgccgaa gtgaaaagcc tgttgaaatc ttgtggcacg aaatgctatg aggttaaatc
     9421 gccggcagct acaaacgaac ccctgcacgg acttatgtgg ggctacaatt acgagtgtgt
     9481 cttatttacg ctatattaaa tctgtagtaa tgactgaact gaagaatcct ttcacacggc
     9541 agcacgattt caattgtttc ggctgttctc ccgacaacag taacggattg cacctgcagt
     9601 ttgctgctga aggcgataag gttatttgcg aatgggctgt tcctgccggt ttcgaggggt
     9661 ggccaggagt agtgcatggc ggtattaccg caactatgat ggacgaaatt gccggttggt
     9721 atatatttgc cattctcggg gttgcttgta tgacagtaaa cctgaacata cattacgaaa
     9781 aaccattgta cagccgtaac ggaaaaatca gagctgaggc tgtctgtatc gaacgacaca
     9841 agcgcatagc aaagattcgt gtagatgtca gtaattccga tggctcactg gcagcctggg
     9901 gtgagctaaa atattattgt ttcaccgagg aggaatcgaa atcgaaaatg aattttccgg
     9961 gaaaagaagc gttcaggtaa caagttattt accacgctga ttccatgtga gtttttgctt
    10021 ttcgttgtag tgtatctttt cgttatcgaa aagccattgt attaccttga tcactttgtt
    10081 gtcgtcgtga ttgatctcat cgatgaggtc gttcagaaaa attggttttt gctgtatgtg
    10141 ctcttttatt tcactcaata tcaggtcgaa ctcatacttg ctcagcgaca gctcgttgcg
    10201 gcgcttacat acatcacatt tcccgcagcg ctcggcgtcg gtttgtccga agtaggcgag
    10261 taatatctga ctgcggcatt tgttggttga attggcatac tgaagcatgg cgttcatcct
    10321 ggtaatataa cgttctttct gcaattcata attgcgcttg ttgatgtaaa ccgattgcag
    10381 ttcgattcgc tccgagagca gcactactac cggtttgttc ttgcgaggta tgtatttgat
    10441 gatattcagt ttttgcaggt tgatcagata ggtgtaaata gtatcgaccg tggtacccgc
    10501 tttttttgcc agatattcct cgctgaaatt cacataatcg ttgaatacac ctgtataatt
    10561 tcgaagcatg agctttataa aagcatcgta ttcttttttc tctacctgaa aattatacaa
    10621 ttcgtctctt tttacaggaa agtgaatttt cgaaggattg ttcaatgcat cgttatactc
    10681 aaggtatcct tcgagctgca gcgtttttag gctgttaaaa atggtgatta catcgagtga
    10741 aaacttcttg gcaaagtcga acatatcgaa ttcgaacgac atgcttctgc ctgcaccaag
    10801 cggcaacccg aagtaattat gcaaagcttc atacaccctt attatgcttg atatttcggg
    10861 aaacgaccgg ctgatgcgct gctttacagc ggtttcatcg gcaggactaa agagtaagta
    10921 cgccaccgaa tcgttcccat cgcgtcctgc ccgcccggct tcctgaaaat aggcttcggg
    10981 tgagtcaggt aagtccatgt gtatcacata tcttacatcg ggcttgtcga tacccatgcc
    11041 aaatgcattg gtgcaaacca tcacactggt tttatcgttt tgccaggcat cttgtttcat
    11101 gctgcggatt tcggggctta atccggcatg atagtaatca gcagacaatt tatactcctg
    11161 cagcataagg gcaagttctt ttgtcccctt acgatttctt acgtaaacaa taccggttcc
    11221 gggtgtattg gtgataattt taaatagatg ctttagctta ttttcggttt gcactacggt
    11281 gtatgtcagg tttttacgtt caaaactctg cgtgaataca ttttctttcg gaaagagcag
    11341 ttgcttttga atgtcggcaa ctacctctcg cgttgcagtt gctgttaatg ccaacaccgg
    11401 aatgccggga aaagatgtac ggatttttgc aatttcgaga tacgatggtc tgaagtcgta
    11461 gccccattgc gatatgcaat gtgcttcgtc cacggtgatg aggttaacat tcaggtaggg
    11521 aattctttcg gtgaaacttt gcgtttgcag gcgttcaggc gatacataca gaaatttgta
    11581 tcccccgttg atcacattca taaaggtaaa atcaatctca tcgcggctca tgccggaata
    11641 gatagctgca gcgcgtatgt ctttttttcg aaggttttct acctgatctt tcatgagcgc
    11701 aatcaatggt gtaactacaa ggcataaccc gtttaccgaa agtgaatata cctgaaaaat
    11761 tattgacttt ccgcctcctg tcggaagcaa gccaagcgtg tcgttgccgg cagcaaccga
    11821 acgaatgatt tcttcctgca gtggcctgaa tttggtatat ccccaatatt tggttaatat
    11881 gtttttataa tcgctcatgc ggaagattaa taatctttaa aacaaattta acattctaac
    11941 gctgtgataa caacattttt tttaatttcg tgaaatttta atgaaagttc acttatgtca
    12001 tatgtttcag ataaaattat tggagaaggc ctgaccttcg acgatttgtt gctggttccc
    12061 ggttacagcg aagtgttgcc gtacatggta aacgtggcgt cgcgtttctc ccggaatatt
    12121 acattgaata cgccgataat tactgcagct atggacactg taactgaata ccagatgtcg
    12181 attgccattg ccagagaagg tggtattggt gttatccata aaaacatgag catcgaagag
    12241 caggcccgtc aggtgaggtc ggttaaaaga gctgaaaatg ccatgatttc tgaccctatc
    12301 attattaatg atcaggcaac agtgggtgat gcactcaacc taatgaagga atttcatatc
    12361 ggtggcattc ctgttgtcga taaaaatcac atacttaaag gcattgttac taaccgcgac
    12421 ctacgcttcc agaataaccc atcgaaaccc attcgtgagg taatgacttc cgaaaatcta
    12481 attacaacaa gtcagaacat caatcacgaa gaagccaaag aaatattgca gcgttaccgc
    12541 atcgaaaaac ttccggtggt tgataaagat tataaacttg taggtcttat aacctacaag
    12601 gacataacca aagctgctga cagcccaaat tcatgcaagg atgaacaggg gcgcctcagg
    12661 gtagcggctg cggtgagtat tgctgcgaat acgcttgatc gtatggatgc tcttgtagct
    12721 gccaatgtgg atgcaatggt tatcgatact gctcatggtc attcgaaagg cgtgatcgat
    12781 atgctgaaag tagcccgcaa gcgctacccc aatattgaca ttgtggtggg aaatatagcc
    12841 accggcgaag ctgcaaaaga gcttgtaaag tatggtgctg atgcggttaa agtaggtatt
    12901 ggtccaggat ctatatgcac cacccgcatt attgccggtg ttggcgttcc gcaggtttca
    12961 gccattttcg atgtggctca ggcgcttaaa ggcacaggcg tgccattaat agccgatggt
    13021 ggcattcgct tttcaggcga cattgtaaaa gctattgctg ccggagcaga tagcattatg
    13081 gcaggcggat tgtttgccgg taccgatgaa tcgccgggag aaactattat ttacaacggt
    13141 agaaaataca aatcctatcg tggtatgggc tctatcgaag cgatgcagat gggttcgaaa
    13201 gatcgttact ttcaggatat ggaagacgat attaaaaaac tggtacctga gggtatttca
    13261 ggtcgcgtgc cttttaaagg tacggttggc gaagtgattt atcagcttat cggcggatta
    13321 cgttcaggta tgggctactg cggctgcaaa gatattgacg aaatgcaccg gaatgcccgt
    13381 tttgtaaaaa taacccattc aggaatgatt gaagggcacc cgcatgatgt aactatcacc
    13441 cgtgaggcgc ctaattatac aagagaaaat tagtaataaa aaaagaaaca gtattatgaa
    13501 aaaagcattc atttttttaa ttgttttgtt tgctgcatgt agccagattg ttgcacagga
    13561 agatgtgttg ctcgaaatcg acaatcataa gatcgatgta aatgagtttc tgcacatcta
    13621 ccaaaagaat aacaacgatg ccaattcgat gacttttgat gcaatgatgg actatatgga
    13681 tctgttcatt aacttcaagc ttaaagtaat cgaagctgaa agtctggggc tcgacactac
    13741 caaagaattt aaagttgagc tcaatggata ccgtcagcag cttgctcagc cttacctgac
    13801 tgataaaacc gttgaggatg aattaatcct ggaagcttat gaaaggatga agcaagacgt
    13861 taatgtaagc catatcttga ttaaagttga tcagaatgca ttgccggaag acacactaaa
    13921 agcgtataac aaagtgatgg gcgtttacga gaggctgaag aaaggcgagg attttacaac
    13981 acttgccatt gccgtatcgg aagacgaatc ggtgaaatac aacaatggtg accttgggtg
    14041 gagaactgtt tttggactgg tatatgaatt tgaaacagag atgtataaga ccgatatcgg
    14101 aaaattttcg cagccctttc gtacccgttt tgggtatcat attttaaagg taaacgggaa
    14161 aagaccagcc aagggcaaat acaaagttgc tcatattatg cttaccatac ccaaagatgc
    14221 aactccggag gataaagtta aagcggaagc aagacttaat gaagtccagg ctaagataaa
    14281 tgcaggtgat gattttgcag cattggcgcg tgaatacagc gaagacaggc aaaccgcagg
    14341 caatggtggt gttttgggtt ggattgacgt tggtggaaaa atgattcgct ctttcgaaga
    14401 tgctgttttt ggtcttactg agatcaatca ggtttcggaa ccattgaaga cttcgtatgg
    14461 ctggcatttt attaagttac tcgaaaaaca ggaaattaaa ccttttgagg aagttcgctc
    14521 tgaattgaaa tcgaaaatat cgaattcggc aagggcatcc cgcagcaggg aagtggtgat
    14581 agagcgcctt aaaaaggagt ataattttac attgaatcag aaagcggcag ctgcgtttta
    14641 taaactggta accgattcta tttttgccgg cagctgggat gctaccgaag cattaaagct
    14701 tgacaacgtg ttgtgtgagt ttggtgataa aaagctatat cagaaagatt tcgccgattt
    14761 tctcgataaa atgaatcgca aacaggtgcc gcagccaata gttgtctttg ttgataatgc
    14821 tcttaaaagt tttatcgata aacaaattat tgaatatgaa caatcccggc tcgaaatgaa
    14881 atacccccaa ttcaaatacc tcatgcagga atatcacgat ggaatcctgt tgtttgagct
    14941 gacagatcaa ctggtgttac aaaggcagtt actgatactg cggggcttca ggtattttac
    15001 gaaaacaaca aggataatta catgtggcct gttcggtatg agattcatca atataaatgt
    15061 aattctccaa aaaatgctgt aaaggttgaa aagctgttaa agaaaggcgc tgattttgat
    15121 cagattgtac ccaagctcag taaaaaggac tcaacagcag ttgtagcggg cactaatggc
    15181 aaatttgtta aattacaatc agcagaaaca gataatatta tttcgaaaag tggtttgtcg
    15241 gaaacacctg gaaaaacagc agtgttttta acagatgaaa ctacggttgt aatggcaaca
    15301 gttatagccc cagaacataa aaaactggaa gaagccaaag gcctcattac tgcagattat
    15361 caaaactatc tcgaaaccga ctggattaaa aaattaagat cgaaatataa gattctactg
    15421 catgaagacg ttcttaaaaa agtagccaag aattagtctt ctgccgtaat taatgagata
    15481 tacattcttt cagagctttt gtgtggtatc cctaattgcg gtagtaatga tatcgtgtgg
    15541 cggaaacgac gatacaaagc tgaatgtagt ggcccgggtg ggtgaaaagc aattactgca
    15601 ggaggatttg gaatttctta ttcctaacag cttttcgaag gaagacagcc taaaatttgc
    15661 agaatcctat atcaacgatt gggtattcga acaattactt tatgcagaag cgttgaattc
    15721 tattaccgac acagcctcaa taaacaagca gctggaagaa tacaggcagt cgctttacaa
    15781 ctactatttt gagcgacagc tcctcgaatc gaaaattgac acggttgtgg gacatgccca
    15841 gatttcggaa tactattcgc aacatcttta tgaatatgtt ctcgaccagc ccgtaattaa
    15901 actgcattcg ttggttttcg acgcaaataa aattgattac atagctgagg taaggatgat
    15961 taaaaatact gaaatagatg aaattgaaag cttgtttgag ttttgtgaac gtgttggggg
    16021 caatatcatg ttgattgaca gatggataag aatgtcggat ttctacagaa tttttccatg
    16081 tgaaggtaac cttactctcg aaaaaataga aaagggaggt tattacgaat gctttaacga
    16141 tagtttacgc tatctgataa ttattgatga tatcatccct gttggctctt actgcccaca
    16201 ggaggtgctt accgatgaca tcagaaagat tatactgcag aagcgctcga aagactttat
    16261 gtcaatctac aaaaaacaac tttttataca ggctgaaaac agcgggcgtt tatttattaa
    16321 aaacaaaaaa aatgaagatt aaaattgcat ttttcatgat tatattgctg ggcactgcaa
    16381 ctgtcagagc tcaggaagtt atcgatcagg ttgtggctgt tgtaggcaat agcccgatcc
    16441 tgaaatcgga tatcgaaggt cagtacatac agatgatgag tcagaactat tattcgagta
    16501 gtgtagatct gaaatgcgaa atccttgaag aatacctgtt tcagaaattg ttgctgaatc
    16561 aggccatgat cgacagcatt gaagttacac caaaagaagt tgatactgaa ctcaaccggc
    16621 gacttgggat ttttattaat cagatgggat cagaagagaa gcttgaagaa tactacaaca
    16681 aatctattaa cgaaattcgc gatgaatttc gtagtattat taaagaccag ctgttaacac
    16741 aaaaaatgca aagcaaactt actaccgaca taaaaatcag cccatcgcaa gtgcggcagt
    16801 atttcaatga aatccccgcc gatagcctgc caattattcc tgccagctat gaattcagcc
    16861 agattgtggt gtaccccgat ttgaatgatg agcagaaaca gataagctac gacaagataa
    16921 atgaactgag ggagcgcatt gtgaaaggag atagttttgc tactctggcc attttgtatt
    16981 cgcaggaccc gggctccgct gctaagggcg gtgagttggg ctttgtaagc cggacagacc
    17041 ttgttcctga atttgctgaa gtcgcattta gcctgaataa tactgaggtg gtttccccta
    17101 ttgtggaaac agaattcgga tatcatatcc ttcaactaat tgaacgtaaa ggagaattgg
    17161 tgaatgttcg ccatattcta attatccctg aaatttcgga tgtagatgta aaaaaggcaa
    17221 aaaaggatct tgaagacgtg tataaattca ttctcagcga ttcgttatca tttgagaaag
    17281 ctgctgcagc ctatagcgat gatgaagatt cgaaatcaaa cggaggaaag gcaatcaacc
    17341 cttataccgg aaattcaaaa ttccttaccg accagattga cgcaaactcg ttgcagatat
    17401 taaaccaact aactccagga cagacgagtt cagttttcga aacacgcgac ctgcgcggaa
    17461 aaaaagtgta caaaatcgtg cgccttgata gaagaattga tgaacatgtc gcaaacctta
    17521 acgacgattt taaggaaata tcagaagttg ccctgcagca ggaacagttt aagagaatac
    17581 aggagtggat atcgcatcag attgatatca catacatatt tattgatgac tcttatgcaa
    17641 attgcacatt caaatatgcc gattggaaca aaaattcaaa aaaataaacc aatgaaaaaa
    17701 gctgaaatta aaactgaaaa aggggtactg aaagttgaac tatacgaaaa ggaaacaccg
    17761 ggtactgttg agaattttgt aaaacttgca cagcagggtt attataatgg cttgcgcttt
    17821 catcgggtta tacctgattt tgtaatacag ggcggatgtc ctttttcgaa aaatgaaaac
    17881 gatcgcaggg ttgggacagg tggtcccggt tacacaatta aatgcgaaac aagtgctccg
    17941 aaacagttcc acgaaagggg tgtgctttca atggctcatg ccggacgtaa taccggcgga
    18001 tcgcagtttt tcatttgcca caaccggaaa aatacccagc acctcgacgg aaaccacact
    18061 tgctttggaa aggtgtatga aggcgatgaa attatcgatg aaattgagca gggcgatttg
    18121 attgaatcaa tcaccattat cgaagaatag ccggcggtat tattatttat agctttcttc
    18181 caaaaactga aggcagagtg gcccttcatt gaaaattaaa tcaagcactg aaagattggg
    18241 cataaaccga aatttttcgg gatatacctg atggtagggt tgaggaaaga agtgaggatc
    18301 ctctgaattt ttttgaattt ttggttgcat agtatagcgc agatcaataa acgatggatt
    18361 actttccgca tattcttttg ttatccgtat attccggtca ataccaatga tttctagtaa
    18421 tttaagtagt attttatagt tttgcataaa caggcttttt tcctgaattt taaaaaagct
    18481 ttccagttca tcaatatagt agtcgtagta agcggattta cgatatgctg attttattgc
    18541 ctgaaagtgg tttctctgcc agttgtagct ataatctatg agaacttccc ctgttttttg
    18601 ccggtccacg gccggtttta taacaggaat gcttaagcag attgctccgt tagctgatag
    18661 tatattgcag cgatttctgt atgtttgccg gtcgtaatta tcataatatt caagataaat
    18721 atcggtgaaa tgtaccagct tactgcaata ttgcaccgaa gggaaatagg atgtggtgag
    18781 caggacgctt ctcatcttca gtaaacaatt ttaccaatga taagatcttg gggtacaaat
    18841 ccaaaagtgc gcgagtcggc accgtgatct ctgtcgtcgg acattacaaa gtagtaattc
    18901 ttattaaatg tgtatttttc gcagacagta tcgtttattt ttattccatg ttgcgatatt
    18961 tcaagggtat tgttttccga aaaccggata atttgttcac tcaatgcaat atttcggtaa
    19021 ttcagataat gagttacatg ctgctgaggt actgccacag gaccgaaaaa atctttattc
    19081 cagttataga agtcgctttg agggaagtag tttgtcgact cttcgccctc aggggttgtt
    19141 agtgtacgaa tattcacaac gccttgagcc gaagcaattg cagacgcatt ttctacagaa
    19201 gtagtcaggt tataggcttt acctttggta attgtcacta ttccccatac ttccatttta
    19261 ccggttagtt cgttgatatc cctattgctg gttgtaagtc ggtattcaaa atataaggga
    19321 taccattcat caaatggctt ttgattgatg tagacaactt tatttttaat ttcaacaatg
    19381 ttcccgggaa gtcccaacac ccttcgtact aaaatttcac ggtctttagg gtcggtgtcg
    19441 ttaacacgag ggtcgtaaaa agctatcaaa tctccatttt caatactcat ttcaccgggg
    19501 agcaatgcgt tatagaaacg gggaaagtga gcagttttcc taattatcac ccattccgat
    19561 ttattggaga aaggcacatc gggatgttgg ataatcgcta ttttgaaaaa tgcaatactt
    19621 accaatatca acatccccaa aataactgct attatccacc atcttttcat ttagtcgacc
    19681 gtgtgaattt tatcaaatac cctgtcccaa cgtatttttc cggtctcttt atctttcgag
    19741 aaccaaatta cccaggcttt acccaccaca tgatcttcgg gtacaaaacc ccagaagcga
    19801 gagtcggccg aattatgcct gttgtcgccc atcatccagt agtaatccat tttaaaggta
    19861 taggttttcg attctttccc attgataaat atttttgcgc catcaatttt cagtgtgttg
    19921 tgctcataat acccgattac cctttcgtac ggtgcgatat tcgatgtgtc gatgctcata
    19981 gtggcacctt tctttggtat ccaaagtggc ccgaaccagt cttcgttcca ttgatatgat
    20041 gagctgtgag gaaatattct gatattccaa ttgccttttt cattaatatc ttttaccaca
    20101 gattgaacat tgtacatagc ccttactttg ccgacatttt cttcagtaag aggtatcaaa
    20161 tattgattgg ttattgcaag accattttcc atggctttca gataattttc tgtatcgtag
    20221 ccattagcgg ttttaatcac aacagacata tcttcgtagt taactccaag gctttttagc
    20281 tcatttgcgt cgatgcctgt aactccctga aaatttccgt attccatggg tttcagtgtt
    20341 acgatatatt tgaattgctt tccggctatg ttcttttgct taaccctgtt aacatatact
    20401 tcgccttcgc gtacttcgag cgtatctccg ggaattgcga cacaacgctt aatatagttt
    20461 tctcttttgt cgacaggtcg tacaattata tcaaccgaag ggtcattcca cagctctttc
    20521 cttgctattt tataatactc tttatcggat aatactggtt tccctttcgc taaatcccgt
    20581 cttttcatat tttcagcttt caccagaatg atgtcaatgt atccttgtcc ctgattctga
    20641 accactaccg tatctccatc aggatagtta aaaaccacaa tatcatcacg ttcaatattt
    20701 ccccaaccgg ctaacctgcg gtagtcgttc tgaatccagg taacaaacga tttggttttt
    20761 tttgtcaagg gaagcgtatg gtgtacaaac ggaaaagaaa tcggagtgtt tggaaccctt
    20821 ggtccatagg caactttgct aacgaaaaga taatcgccga taagcagagt tttttccatg
    20881 gaacttgttg gaatggtgaa ggcctcgaaa aagaaaatac gtatgaacga ggctgcaaca
    20941 actgcaaaaa tcaatgcatc aacccactca agagtttttc gcttcagtgt gctcattttt
    21001 ttatcttttc gaggcttcca gaaagcccag cgtacgaaac gggtgatata aatatcaatg
    21061 attaacagtt ccccgatgag ccaccagtaa ttacccaacc atactaccca taaaacgaac
    21121 agcacagtta caagtccaaa agcaaaatag cggtttgtaa gaaaagcacc tgttttcgtt
    21181 ttggcaaatt ttgcccaaag acctttccgt ttttcctttg tggctttagt tcttgatcct
    21241 aacaatgatt tcgattttgt ttgttttgaa attgccatta ttttatcttt ttgttagtaa
    21301 gtcgtgcata ccaaaaatgc cttttttccc gatacagtat tgagcagcca taattgcacc
    21361 aagggcaaag ccttttcggt ttttagcaga atgacggatg ctgatatcat caatatcact
    21421 ttgccaaaca acatgatggt ctcctgtaat ttctccttct ctgaacgaaa aaacaggcaa
    21481 atccgatatg tcgtcgcctg aatttaactt ccatttaaca taacggttgt ggcgggcaat
    21541 gatatcgttt gctattgaaa tggctgtgcc acttggctta tcaagcttgt gtatgtgatg
    21601 ggtttcatca atatgggcac tatatgccgg aaagtcagaa ataattgttg caagttggcg
    21661 gtttatttcc atgaaaatgt tcattccgat actaaaattg gatgcataaa aaaggcaagc
    21721 attctttgcc ctgcacaatt ccgatatttc agggagtttg tcgtgccacc cggttgaacc
    21781 gcatatcacc ggaactccgg tttcaaaaca cagtgcaatg ttggcgggag ccgattgggg
    21841 cgaagtgaac tcaataattg catcaacctt acgcagacaa tctgcgttca atgacgcgat
    21901 gttattggca tcgaaaatca aaggaacttc atgcccctga cttaaagcca gtgcttcaac
    21961 ttctttaccc attttaccgt aaccgatgag tgccagtttc attgtctgta atgttttttg
    22021 taaaaataga aaaacatatc gtaaaacatg gagcattact caaactttag cccatataaa
    22081 ctattccctc tgagaaaatc gggggtattc attctttttt ttccttctgt ttgaatgttt
    22141 agcagattta taaatccatt ttgcacggca acttgtatat aattgttgtt atcagtgata
    22201 atggttcccg gataatgttt gtgttgttca atgctaaatg tggattcgaa tattttacaa
    22261 atcttcgaat tcccatgttt gtcagttaaa tgtgaaaacg ctcccggata aggactcagc
    22321 cctctgataa aattatatat tgcctccgca ggcatattcc aatgtatttt acagttttct
    22381 ctgaaaattt tcggtgctgt ttttagtgtt ttttcaccca gtatcacgaa actttgaggg
    22441 atgggtgtta ccgtgttttg ttcaattgct ttcaacgtgt caacaagaag tgtggagcct
    22501 gctgacatga gtttatcatg caaagtaccg gcattgtcgc aactttcaat tctaacttct
    22561 cttttaaaga taatattacc ggtatcgata ttttcgttta tgaaaaaagt tgtaacaccg
    22621 cttacttctt ctccattaat gatggcatgg ttaatgggag cggcccctct gtactgcggc
    22681 agtagtgaag catgaaggtt tatgcagcct ttgggtggta tcttccaaac ctcggtgggt
    22741 agcatcctga aagccaccac aacaaaaaca tcagcatgaa ggttttgtaa ttgattaata
    22801 aaatcatttt ctttcaaacg attcggttgc aaaatgcaga ggttattttt gatcgaatat
    22861 ttttttacgg cagactcact catagcaagc cctcttcctg ccggtttgtc gggtaccgta
    22921 acaaccgcag caacatcatg gccggcgttt actatcgcat tgaggggggc gacagcgaat
    22981 tcgggtgtac ccatataaac aattctcatg ctgcgtatgt tattgattgc ctttgaaatg
    23041 caagtcgtgt ccaagttttg acttcttggt cttcaggtaa aaaagattga atttgttggg
    23101 ttcaatttcc agaggaatgt tttcgactat ttgtaatcca tagccttcga ggccggctct
    23161 tttttgtgga ttattggtaa tcagtttaat attatttatg cccagacacc ggataatctg
    23221 agctccaacc ccatagtccc tctgatcggc tttaaatccc agatgaagat ttgcttccac
    23281 agtatcgtaa ccatcctctt gtaatttgta ggcagcaatt ttattaaaca gtccaatgcc
    23341 tcttccttcc tgattcaaat ataccaaagc tccttttcct tctttttcaa tcatcatcat
    23401 cgatttgtga agctgcggac cacagtcgca acgcaacgac ccgaaaatgt cgccggtggc
    23461 acaagaggag tgcatacgta acaacacggg ctcctcaggt ctccagctgc ctttcacaag
    23521 agcgatatgc tcaaggtttg ttcccttttg cttgaacgga atcagattaa aaacgccaaa
    23581 ttcagtagga aggctaactt ttacgccctc ttccacaaga ctttctgttc tgagccggta
    23641 cgtaatcagg tcctgaattg ttacaatttt aatgttatat ttttttgcta tctcaataag
    23701 ttgaggtaac ctcgccatgg ttccgtcatc gttcattatt tctaccaaag ctccaccggg
    23761 ggtgagccca gcaagtcgtg taaggtcaat taccgcttcg gtatgtccgg aacgttgtaa
    23821 tactccgttt cccctggcca ttagcggaaa aatgtgcccg gggcgcccga ggtccgacga
    23881 ttttgttgca gggttaacta atgccgcaat ggttttagct ctgtctgctg ccgatatacc
    23941 cgtggtacac ccttgtccta ttaaatcaac actaattgta aaaggtgttt catgaagtga
    24001 agtattcttt cccaccataa gctcaaggtc cagctcttta cagcgttttt cggtgagagg
    24061 tgtacaaata aggccacggc ccacttttgc cataaaattt actttttcgg gtgttatggc
    24121 ttcggcagcg acgataaaat cgccttcgtt ttcgcgatcg tagtcatcaa caacaatcag
    24181 aagttttccg cttcttatat cagcaatggc ttcttcaatt gtattcagtt tttcactgaa
    24241 atcgtttggt tggtttccag acattgtttt taactcctat aattgcttta aaaaatccta
    24301 tgaacaaggc aaggttcatt gcgtaaaagt gagtaatgaa ccgcaaataa gtattatgta
    24361 tcccattctg tttaaagaat aaatctatca ggggtattat aaaactgaat gcatacaagc
    24421 aggcaagcac caaataaaaa gtattgaaaa atgcgagtac tgttaggcat gtcaacgcaa
    24481 tgatcaaaaa gaacggaccc agccatcgca gaactttatg tgacaaaaaa ctgaaagcca
    24541 ggcctttggt tcccgagaac aacaatcctg caaaaattct aaggttttga aaatcaccgg
    24601 ttgctattct gattttacgg cgaaattcta tacccaaatc gtttgaaaca tcttcgtaaa
    24661 caaccgcctc aatattattt actgctttgt atttttggcg aagcacatgc atacaaataa
    24721 agaaatcgtc aaccagaagg ttagagggca caggcacgaa aagttccttc cttagcccat
    24781 agcatcctcc aaaaggcccc atcatggttc cccatagatt gctttccata tttttaatag
    24841 caacctcacg cgaaatatat gatttctcct gcatgctgat gccttctttt ttgaggtttg
    24901 tgttcaccat atttgagtcc accagtccga ttttattatc agtcatcggt ttaatgagtt
    24961 caggaatggt ttcaggagtg aaaagaacat tggcatcggt aagaatgagt atgtcaccgt
    25021 tggcataatc ggccagttga ttgataatca tagctttccc ctgacgggtt gagaatgtgc
    25081 gaaaatgaat gcgcggatcc ttagaggcca tgatttgcag aatactgttt gtgttgtcgg
    25141 agctgcagtc agagccaatc cagaattcaa ttctgtcagc agggtagtgg gtgttgaata
    25201 cggattgaac cttttgttcg ataatgtcct cttcgttata agccgacata agaatgctta
    25261 cgaaaggttg tgtctgattt gaatattttg cctctgctct tttttttgag ctcagaatcc
    25321 gtaacagtaa tggatataaa acgtaggagt gaaaaaccgc cagtacgcaa aaccagaata
    25381 tgacctgaag ggcaatcatg tactatgggt cttataaaat tcggatagtt tttttgccag
    25441 aacaatattg tcgtagtttt cgcggataaa actgattgcc ttttggctta tcttggcgca
    25501 gagattttta tctgtaatta acctttcaat ttcattaacg aacgagtatt cattatcggc
    25561 aatgcaaatg ttttcgcggt gcgttgcagg tatgccttct gctccgattg gtgtggtaac
    25621 aattggcttc ccgagtgcca tgccttcaat gattttaatt cgcattccag aacctgaaag
    25681 caggggtaca atcattatac catacgactt cataaactct tttgcgtcag gtacttcgcc
    25741 ttggtaggta accccctcac gcaaaacttt tttaagaaac gacgcagaag catttctccc
    25801 tgcaatatgc aaacttacct ctggtagttt cgttttaaga atggaccagc aattattaat
    25861 gaaccaaagt aaaccttcct gattgggtac ccagtcgagt gcgccaatat aaaacaattg
    25921 ttcaggattg gaaccgattt ctcctgcact aaattgaggt atatcgatgc aagccggcat
    25981 aatcatcgac ggttttacat tccccagccg gttgaaaact gcttcatctc tttcggtaat
    26041 aggtattaaa agatcatatt tattaatggt tctttttttt agctcaacaa ttctcttggt
    26101 catgagctgc aggtactttc tcttcacata atttttttcg ttattggcag tgcgttgcca
    26161 tatttcgtgt tcaatgttat gtgaacgtaa cgaaatcaat gcgttgctgt tgttttttac
    26221 cacatcaata taaacgcaca tatacaagcc ttcgaattgc acaatgtcgt aattgtgttg
    26281 ctgaagaaga tctatcagtt tttcttcata ttgggctgaa tagaacctag atgccatgta
    26341 gggcaacctg gagaatagca ggttcctgat taagccaaag aaagagatat ctgtttttag
    26401 aaagacgctt tctaaatgaa gtttttttct tacttcatcg ggaatctggt catggcttat
    26461 gtagtgtttt ggtgtattaa ttgccagtac atgcacttcg ttaccgacag agcccagtga
    26521 atcgacaatg ttataaatgc caatagtacc accgtctttg ggcgggtacg gcattttatt
    26581 cgatagactg agtattttca taaccacgac gtactaaaca aaataatttg cgaatttaat
    26641 aaaattagga ttgctctcct ttctttttaa atttgcgttt aaatttaaac atgttattca
    26701 gtcggttata ttgctcattg ctgacaataa gagagtcgtt catggccttt aaggtcaggt
    26761 aaaacccaat cggaagcatg acaaaagccg aaaaccacat ccctaaaaac ggtgtaattg
    26821 ccagctcacg tgccattttc tcgaaagtaa tggaaactac ataatataaa agaaaaacaa
    26881 aaaccgatac aactaccggg gttccaaatc cccctttccg tattattgcc cccaaaggtg
    26941 ctcctacaaa aaacaagata atgcatgcca gagctactgt gaatttccga tgccactcaa
    27001 tgctatgcct tcttatccat tttgttgaat attccaactc atctcccgta ttactgatat
    27061 aagtttctgc cgagcgactg tagttcagcg caaagtcaac aactttattt ttttgcgatt
    27121 ggcttaggct attgtacagg ctatcgaaat taactgcttt tacttcttgt tcatttttaa
    27181 ttatacctgc catgctgcgg ttgcgttgcg ttaaactgtc gaatttgcga acaataatcg
    27241 gtgcattagt aatttttggg gtttgtccgg attcagggtt ggaattcttt tttcctttaa
    27301 acatgctgta tagcaacagg ttgtttgcta tatcgcgttt cctgttcaac aggcagttgc
    27361 tgagcgagtc gatagagctt tgcagctgcc tgatgttaag catttcgaac ccatttttaa
    27421 agaggtcttc attattcctg tccagcccca ggccgtcaag ctcaatgtac atggtttcct
    27481 gaacaaaaga aacccgctgg tgtggatagg ttgaagactg ttgtttgttt ttgggattga
    27541 attcaagctc ctcataacgt gttccgttgg tgaggctcag tatcatatgc tttttgttcg
    27601 atgttatttt aatagaaccg ctttctgcag tggtaacatt tttattacca ttttttgcac
    27661 gatgatcata aatcatgatg ttatgcaata cctggttttt gtctttgttt tccactttga
    27721 tactgaagcc atcaacagct tcagtaaata ttccgttctg aagatttatt tcaggtcgtt
    27781 gttgctgtat gctgaaaagc aatgacctca ttttaaggtt tgtgtaggga agaatgttat
    27841 tagtaaagaa aaatgctccg acagctatac ctgcagttaa aacaaccagt ggatacataa
    27901 tacggataag agaaatcccg cccgacttca tggcaaataa ctcatattgc tctcccagat
    27961 taccgaaagt cattagcgag gcaagtaata tcgatagtgg cagagccaaa ggcaccaaag
    28021 ttaacgatgc atagaacaac aactccagaa ttacaccagt ctccaatcct ttccctacaa
    28081 gttcatcgat atatttccaa aggaattgca tcaggagtag aaaaatgctg atgagaaaag
    28141 ttagcacaag aggtcctgca aacttaccca gcatatataa atgcaaacgc ttcagcatat
    28201 taaataagta ttctgcaaaa aattaatgca aacatagtga taaaagcaga aaaaatacta
    28261 tgtgccgata attctcttaa ggtcggcaat cagggtatcc caaaggtgaa tggcttcagc
    28321 tttttcatgt tcagtgacaa aatcagtaac aaccagcgaa atgctaccgc taagttcttc
    28381 cgaaaccagg cggaattcga aatagtgatc agcagggctt tcgttccacc tgaaccgcac
    28441 ctgtttcagg tcttttattg caatcaattg tgcaggatgc gattgcttat cccaaataaa
    28501 atcatacaca tctcccatta acttaacatc atctgcaaac cattccgata gtccggcggt
    28561 ggaacttaat ctctcaaaca atatgcgtct ggagcaattc acaggatatt caagctcgat
    28621 tttaatcttt ggtttgttct ctgccataac gtaataaact cctttttgca atataggaaa
    28681 ataaaaaaca gtttccaagt attgaaaatt taaatttttg ttatttttgc caacctgaaa
    28741 taaggcgagg tagctcagct ggttagagcg catgattcat aatcatgagg tcggcggttc
    28801 aatcccgcct ctcgctacag gtgatgtcaa gggttgcaga gttttaagca acctttaatt
    28861 ttaaaaatta acaaacaaat taacaaacat tttactgtat ataatacaat aaaacaatct
    28921 atggctttat atactagata tggatcacta gagaaagaag atgatgatat tgatattatt
    28981 caaacaattt cagaattaaa aaatttactt aaagatgtaa agcatattaa aaccggagat
    29041 ctaaagtcaa cttacattaa acaaataacc cgcatagcaa aagagttctg taaacaaaag
    29101 aaagtcccct tcaaaaattt atgtgaagat agcatgctag ttattagttc agaaagttct
    29161 tattttgaaa ctgctattga agctgaccta atatggcgcc ctgaatatag tctcgaactg
    29221 tacctgaact ttcagttgaa taactataag ggtaaaaagc taaatgataa gagaacattt
    29281 gtcaatttga tttctcgcaa tgtgtgtaaa cgaatggaaa aacgtagtcc atttaatgtc
    29341 aatgatagac ttaaaattat tgcgaaatgg gctttagata agaagaatga attaggagaa
    29401 ccaccaagtc taaaaatgtt agatttcaca aaagatgtca agttggatct gtttaatgat
    29461 ataaaagatt attttataga aaagcagaga aaaagactct tgaaattgtt gaatggagaa
    29521 ccaattagag gtaaaatcgt gtttattggt ttggcaagtt ctttaattga tgtattctac
    29581 aggttacata atgaagattg tatagcctca agtaaaacaa atacagcaaa ttggattgct
    29641 cagcacttcc gatataaaac agatgcaatt catgattttg atcgcgataa tgtttataat
    29701 gggatgaaaa cgaaatattc aaatcgtcgt ccaaataatt caagcaaaat tgaaatttca
    29761 tttctcaaac ccattgatag tattaagaga taggaaatct ctcttttttc tttagaattc
    29821 catgttccat atttcaattg gaacattctg cctgcaatat ctttatagct gattttagag
    29881 cacagattat gccagagccc gattcattta caaaaatatt caactcgttt gaggatagat
    29941 taacttccat cgttcaggag gccgttttca aagccatgtc cgattattat gaaacccatc
    30001 ctataccaaa ggcagaagct accctggaag atgatttaac agatgttaaa ggtatagcag
    30061 cttatacgaa atactccgta tttactatct acgcctacgt agaacagtcc aaaatcccct
    30121 actatagaca gcctaactgc cgtcgcctaa tattttcaaa gaaggaagtg ttggaatgga
    30181 tcaagggtga aaaaaatacg accaaggaag aattggttgc tgaagccaga aatattaaaa
    30241 ttcatgtcag aaaaaataaa cccagtaata aataatacaa tttatgaaaa aattaattaa
    30301 aatgaaacaa gacccttaca ccggagactg gtttgttcca agaagatcga atcagaaatt
    30361 tgaaactcct gaaaacaaaa atgcatatca caataaaaga aaaaaagagc aaaagcaaga
    30421 agacgatctt cttgttaatc ctatcttgaa aaatagaaat attcttatgg aattaatgaa
    30481 agacaaaaaa attggaagat ttcacaaaca attcttatta ggaaaggggt ttgaccttaa
    30541 aataatttat aaaatgcgtc aagaaggtaa caattttatt tatttgattt ccggttttga
    30601 attgattatt gacaataacc acattaaaat tattaatcat gcaagcaaca gcaataacag
    30661 acattaaaat tgttaattat ggatcaggta tagagaaatt agtgagagaa aacagtgcac
    30721 tttcacaaga taacaaaagc ctaagagtat tggtaattgg aatctctgtt attagtctaa
    30781 tatgtttagg ctacatcgtt tattcgataa ttaaagaaga acaggaaaag cgtaatagaa
    30841 atattttgaa taattctgta aaataaaagc gcttaatatc tttgttttac actatgagta
    30901 agaaaatgct attcttcaaa tagaggtcta tcggaatatt ttttacaatc aggttcgttg
    30961 cagccatgtt gcgtagctta atttgcttaa tccgatgggt gtaaatttgt ttgattccta
    31021 aaaacataca tttggtactt aacaagttag ttttcagttt gcatcgaacg gattttagta
    31081 aatatccgta ttgtttgacc ggtaataccg attgccctgt caatacttag tatttctgac
    31141 agctttgccc tcctattctc tataagcgta tagcaaagtt ccaatgtttt cgtttgctgt
    31201 actaagccgg atggcagtag ttctcaacac gttctgtccg gttaaaaacc aaaataaacc
    31261 gtgactgcga taagctgata taagaaatct gtgcttttac gtatcgacaa cttaatgact
    31321 aaagctcagc acaagtgcct tactaagccc tgtgttgttg ttgtttatta ccggctactc
    31381 tcaactcacc ctggctcggc tctggtaagt gcagctaaac cacatttgta ccgtacctcc
    31441 ggatatttgc aaagtttcga taaaacaatc ctcgatattt acaatttatc atttcataaa
    31501 agactgaatt atcaatatgt aatagattgt attaatgtgc aagattatgt atttgttgca
    31561 catttataca acataatgaa tggagcgatt taagcgaaaa tcaaaaacca caagaaaaca
    31621 gctttttttt gcactaaaaa tttggagtag agtaaaatcg ggtgaaagtt atttatgcgt
    31681 gtaaacgctg aatatactga ttaataaaaa tgaatttacc ataacaatct tcatctcacg
    31741 catcccatgc aattacatgc attcacccgc aaagtttttt catcgtttca tgtcatctat
    31801 tttttcaaat tattaggatt gtcgttgttg tcacaattgc actatttgct atccttttta
    31861 ataaagattt gccgtatatg gcaataatga aagttatata ttgcacaagt gtacaagaga
    31921 atgaatttga atcattttat gaagtctgaa catgttggac aaaattcccc aaatgtgcaa
    31981 gattatgtat ttgttgcaaa tttatacaac acaatgaatg tagcgattga agcgaaaatc
    32041 aaaaaaccac aaaaagtagt ttttttgcaa aaccaatttg acgtagcact atttggtcat
    32101 aaaatgattt aatacagaat atgttgatta ataaaaatga atttaccatg acaattttca
    32161 tctcacgcat cccatgcaat tacatacatt cacccgcaaa gttttttcat cgtttcatgt
    32221 catctgtttt ttcaaattat taggattgcc gttgttgtta caattgcact ataagtcatt
    32281 cttttaataa agatttgcga tgtgcgatat agagcaaaat tacatttgta taaatgtgta
    32341 agatgttgtt ttgtaagctc cccaaaactt accccctatt tttcaataat gaaagacaaa
    32401 aaacaacatc tgagattgat ttatcatagg catttttgtg tcagttggat ataccgctca
    32461 aattgaccac ctttcgcagt ttaaaattga cctcccccaa tgtgtgcaga acaacttgat
    32521 actaccggtg aactcgtacc agaaaatgaa tgcctgcctg agagagattg ccgacttgtg
    32581 cgttatcagc aaaaaccttg agtgccacct cgcccggcac aactttgcca ccactgtagt
    32641 cctcaacaac gacttaccca tcgaaaccgt ctcgaaaatg ctcgtccata cctcactcaa
    32701 cgtcacatgc atctatgctg ccgcctccac gacaagaaaa atggccagga tatgaatcat
    32761 ttggacggga tgtatcagaa gtaggggtga tgtgtgtgtt atatataaca tccatcttat
    32821 tcattcaaca ttaatgtttc catagtaaat ttcggattca ataagtcgat gtcgacgggc
    32881 tttgggattc cttctattgt gccggattcc gaaaattgcc atattgtcca atcatgcttg
    32941 tctctcatgt tgggactgtt ttcgccatat cggcctatta aaaactgata gtcgctgtac
    33001 tttcccgata aatttttatt atagaagcta tttacagtgt aaattaaagg ttttttgcca
    33061 tagtgttttt ccaccataac taaaaacttc ttaaagttct tgtgaaaggt tgccgaattc
    33121 caattcttgt tttcttcaac gtcgaccaat ggtattaaat cctgagcatc tttagccaca
    33181 tttcttttga agtttttgaa ttgttcttcc ggattcgaag ttgttctgaa gtaatgatag
    33241 ctgccaacca ggaaaccctg tttttttgca cctttcacat ttgcctcata gcgcttatca
    33301 acataggtac tgccttcggt tgccttaata taaatgaaat gtatcggttg cccttcccag
    33361 tcttttactt tcgaccaatt gatttccccc tgatgattgc ttatatcaat cccgtaaaca
    33421 gaatcaccgg caggcacaac caccttttga cttttttttg aattgttgca acccgaaagc
    33481 aaaaccatga gagtcaacat aaatggcatt ataggtttta attgcatcac aacgtatttt
    33541 tatgtagcat acagcaatac cggaatatct atcaatagcc gtttatattt ttttcaataa
    33601 atcatctgta gtacacggtt ttttatactt actcaattcc tttaataaaa tagcgttgga
    33661 aatataattt tcgaaaaagc ttataatatc cggtgtttca atgctaaggt ctttaggtaa
    33721 ttgcaccttt tcaataaaat gttcataaag cttatcataa accttttcat atatgtgcaa
    33781 ccattcgaca tagaccgaat tttgcacatt tttagttttt gtaatagcga ataattcttt
    33841 gtttgcgttc tggcactgtt catttagttc gttaacccct acagcaggta taatcggcga
    33901 cggataaatg tagtattttt ttgtgcccac atcatatctg agatcaaaac ccaaacagag
    33961 ttccattatt gtatcacaga actgataaat cactgcgtat ttgcttttag atttctccga
    34021 aaaatctgcc caatttaata aaaggatatc cccatacagc tctccgtatt taaataacca
    34081 atcataagtt tcaccgtcat catctctgaa acccggttgc gaaatctctt ccatagctgt
    34141 ttttgcagcc ttgtaatagt attcgtgtgt tttgctcatc ttttttaata tttgtcgaaa
    34201 ttagccttta cctaattaag tcctctaaac gataaaatta aacaatttgt acatgttttt
    34261 ttccactgta ttgtaggctt cccattttta gtacagttca aaaatagaat aaatgattca
    34321 tatttcaata actttctgtt tctagaaggg ctttgtggag cgaaggcgta gccgaagcgg
    34381 aacaaagccc ttctagttgc tgcttttttc ctttaatatt ccgatacatt accggtggca
    34441 atccacctaa agaatcgtga ggcctgaaat ggttatagtc ttgcaaccag gtttctgtta
    34501 tctcccttac ctcgttaagg ttttcaaaca aatacgcatc aagaacatgc tttctgtaag
    34561 ttttattaaa tcgttcaaca tatgcatttt gagtaggctt tccgggttga atatacttaa
    34621 actctatttc attcatttcg ctccattgct gtgtgatttt tgctacaaat tcaggcccat
    34681 tgtccatcct gaccttttca ggcttgccat gtcggtttat taagtgccgg agtatccaga
    34741 tcactcgact gctctttaac gaatagtcgg tttctatata aagtatttcc ctgttgtagt
    34801 catcaattac attgaagctt cgaaattttc tgccattaat taatgcaccc gaagttgttt
    34861 ttgaaagaat tccaatgtat acatgaaaaa aagaatgatt aacgctgcga tgttgaagaa
    34921 ctgaagtata gagaacttgc attatctcaa aaaaaacatt tattaaaagc caaacatctt
    34981 gtcgcgtctt cggatatact tgctgtaggc aacttcacgc ttaacaatat actctgtaat
    35041 tctctcttca tattggcggt caataattcg cttctctttt tccaatgcaa tgttaaaata
    35101 ttggtattga atcaacaatg ccaaattaac ctcgatcata tgcttaattt gaaaagacca
    35161 aagttccgaa accatacgtt tctcattgat gtcgtcagaa taaaaaatct ccaattcggg
    35221 caaatcatcg taatatttgg ggaatttcga cggctcatga aaaaaaatat aatgagtaat
    35281 aggagaagaa acaatcaatt ggagaagtaa gtcccaatcg aattcatcct taagttcgac
    35341 gggaactcta taaggaatgg gtgcaaattt cgtgagataa tttttgtaga atttttcatt
    35401 ttgtaacgac aaagacccgt tgttttttac ccaggcaata tatgcgtctt tgaacaatac
    35461 atcctcgctc gttttacatc tatctaaata ccctgcataa tcaaattcca tgtcgaaata
    35521 tgtctttact attttatact aacctatgta attacttgtg atattttctg gtttcttaca
    35581 atgcctatca tagtatggta ttgttaatga tttcttccga tgaaagaacc tcgattttgc
    35641 gatatcaaca gcaataatta ccaaaacgga attttcgtaa ttgcttaagc caacgattga
    35701 tagctgggaa caaaacgatg ttgaattatt ccggcgaaaa tttgagccac ttcggttact
    35761 gaatacccca acattttagc cccaatttcg aaaatcgttt tcacttcaac attctttagc
    35821 tcgttatcgg ccataacaat ttcaatcatg tactgcagca ttctttcgcg ctcggcaggg
    35881 ttgattttta gtattaattc gatggattgc tgaaaaaccg acatagcttc tccgcttttg
    35941 gcaatacctt cgagaaaact tttagggaag attcggaatt cagccagatt ttgaattatg
    36001 aaatcaattt ccttatcagc tatgtcttta tcgcaacccg caattatgag acctgcactg
    36061 gcaataaaat aggttaaatg aacatcaatt tccgaacttc tgagtttgag caaaatgtca
    36121 atcagctcgt ccatgtttgt ttcaatctct tcattggtta aagcaccttt tttaccgggg
    36181 ttgcatttct ctgactgaga aaacaattcg agggctttta ccctgatggg attgatgggg
    36241 tgggaggcat aattcaatcc ggtgctgttt ttaaaatact ctaacctttt attgttttct
    36301 tcgataaatg cctcaacgtg caaatcaaca cggcccgaat caagtcccga cgacatttta
    36361 aagaaagccg atatgcagac atcaatatca ggcattgcca taaaaccata cctgtcggca
    36421 atgagctccg ataactgatt ccataaccgg attttattac ccagcaaggt tggggcattt
    36481 tcgggttccg ggaaaataaa gttgatcaaa cgcattaatt cagtgttctt atttatcaga
    36541 tgacccattt cgtgacctat aacaaaccgg agttcatcgt cggtcatgag ttgtatcaac
    36601 gatgaattga tattaataat gtgaggttcg ttttcttcta ctcccgaaag ggcaaatgca
    36661 ttaacattgg cattccccga aatatagaaa tcgaccgaat cgccaaaccc aagttcattt
    36721 tttacctcat ggaacaaggc atacaatttc gaagatattt gtttttcaac cttgaaactg
    36781 tgcccttcca gaacatttcg ccaatagttt tcattttttc ttattttagc aattctcaaa
    36841 atatcgtcaa caacatcgcc ttgtaaagcg gtgtaaattt gatgtcgcag atactcttcg
    36901 agggcaatgc catacccgct atcgtcaatt ttcttattca taataatttg gtgtaaaagt
    36961 atatcagcaa atataaataa ttgtaaataa aacattccta tttgctgtta attatatttt
    37021 attttgccag aactgggtat ttcattacga atcgttcagg tttacgatac gaataaaaac
    37081 gaaacaccag atttgaatcg atgataatgt taccaagctg agtggcgata atacaaaaat
    37141 aaccgcacca ttaacttaca gaagagctca gcggacaccg agcgaaatcg aggtgcccgc
    37201 attgcgttgc aataattcaa acgtatcagc ggtttataac cctctgtcct cactccgtcg
    37261 aatcgagcct tatcgagata acaccctcac tctgttttat gtagccatct ttcaggctta
    37321 gcggtcaccg agcgtagtcg aggtgcccgc tcttcagaag ccatttcgca ggctcattca
    37381 taatcaaaaa aatggcaaat tctaaaattg atttttcgac gtttcagtag ccatcttgca
    37441 ggctaatagc tatggttcga ctacgctcac caaccgcccg cctccagatt ggcaaatgtc
    37501 tggttttttc agtcgcaaca aaagtctctg tactcactca cacactcact ctgttccagg
    37561 tagccatctt gcaggctaat agctaatcta tttagttttt tgttcatcag acaactatga
    37621 atgtttcagt agccatcttg caggctaata gctatggttc gactacgctc accaaccnnn
    37681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc cagattggca aatgtctggt
    37801 tttttcagtc gcaacaaaag tctctgtact caatcacaca ctcactctgt tcttggcagc
    37861 catcttgcag gctattttgc aaacgcttgc ggtcgccgag cggagtcgag gtgcccgctc
    37921 ttcagtagcc atcttgcagg ctaatagcta atcacgctta ccactggagt aacaatcagt
    37981 ctgcccaagt ttcagtagcc atcttgcagg ctaatagcta atcatttgcc gcttgaccaa
    38041 aatttaggtt tcgcctgttt cagtagccat cttgcaggct aatagctaat cagggacggc
    38101 atcatgccga attgagcatg aagcagtttc agtagccatc ttgcaggcta atagctaatc
    38161 aatgacctgg aggaggcagt aatgcctcaa cgagtttcag tagccatctt gcaggctaat
    38221 agctaatcat gatggatgta acgcatgtaa tgaaataatt cgtttcagta gccatcttgc
    38281 aggctaatag ctaatctaaa aattgaaaaa ttcgctgacg gcttaacagt ttcagtagcc
    38341 atcttgcagg ctaatagcta atcattatga gcataccaaa gtagctctga aagcaagttt
    38401 cagtagccat cttgcaggct aatagctaat cttgaaaagt acgaaccaac gggagatgaa
    38461 cccgtgtttc agtagccatc ttgcaggcta atagctaatc tgtttcactt aattaaaaaa
    38521 taaaatgatt aaagtttcag tagccatctt gcaggctaat agctaatctt tcattcttat
    38581 atactaaatg agggttcgaa gtttcagtag ccatcttgca ggctaatagc taatcaggat
    38641 actttaaaaa agaaccgttt tcggaaaagt ttcagtagcc atcttgcagg ctaatagcta
    38701 atcgttgaac aaataattga cagagccagt aatgggtttc agtagccatc ttgcaggcta
    38761 atagctaatc aatttgattc tcaacacgtt ggcaaatata caacaataat ttgaattatg
    38821 caaaacgtca aagaacccat tgataatacc acaaatttat aaataaattc tgaggatttt
    38881 cgcaaatcta ttgtttttat atgataaatt ttactgtttt taagtttaaa aattgcgtaa
    38941 gtacaagtaa atcaataaaa taagccctta aaaaattttc gcaaatctct tgaaaaaatt
    39001 ttggattttg agatttgcga aaatcgggcg tttttaggca aaaatgagta tgtgttaagt
    39061 gtcagattat aaggtgatta cgaaaacagt aaaagctgct aaaagtggtc attttcgcaa
    39121 acctcggcgc gattttgtga aaaccggaga tttgcgaaaa ttttatttca tgtggatttg
    39181 ttgtaaaaaa tcgtaatgtg ttaataatca gcatgtaaga cgttttttcg attttcgcaa
    39241 atcgttttca gctgtgcagg agggtttttc catcggcaag gaactgggtt ggagtcaatt
    39301 tgaataaatt tacttttata gtaataaaga tggaattata agaacaaaat atatattttt
    39361 aaggcaatta tctgcttgcg aaaaagtaat agttaatgca tggaggcgca acaaaaaata
    39421 ttcatctcct actcgagcaa agatcaggtt caggcaaatg cgttatgttc gttcctcgaa
    39481 gggtcggggc taaattgctg gattgctccc agagatattg tgcccggcaa tatatatggc
    39541 gaaaaaattg ttgaagccat caataattgt gatgctgtgc tgttgttttt caccgagaac
    39601 tcaaaccggt cggcacatgt ggctaacgaa attgacattg cttttaatgc aaacaaacgt
    39661 attattgtat ataagctggg aaattttagt gaatctccgg cgttgatgta ttatttacga
    39721 agaacacaat ggatcgttgc ccggaatgca ataagcgaca atacattaat tcttaaaaca
    39781 tgccagaaca acccctgcaa cacgccaaaa actctcgatt tctccaatat ctgcaataaa
    39841 atagcgactc agataagcca atttgcaaag caaaaaaatc atgagtcaga aacaagtttt
    39901 acccgaagtt tttacagttt caacaaaaaa ctgatttcat tcgagaaaaa tgattgggtt
    39961 tcggttgtct tcgtaattgt ttttattgtg ttactcatcc gaatattcgt accggtaata
    40021 gtatttgacc atagttctat aagaacatgg ccaaaattgg tttcgttttt ctatcacacc
    40081 cgcaatcttt taactttcaa agcaattgga atacttgcca ctattgtaac aggcattctt
    40141 ataccgccat tgttaattct gattcgaaaa ttgcttaaaa ataaattcag ccttgtacta
    40201 ctcggggtcc taatgattat cagtcttctt tttgctgaaa taaccccggt gtttcttttc
    40261 agaaactacc atttgagtct tggctttaac gattttagat atttttcgtt ggctaccgaa
    40321 atgttgtttt taaccccctt gattcttgta attggttgtg gtccctttgt cttgcattat
    40381 ttgtacaggc aaattgaaga gtctaacctg gctttcgatc ggaaaatgga gtatctgaaa
    40441 accgtgattc ataatgtaac ccattgcgaa aatgacccgg aacggataat atcggaactt
    40501 attcaagaaa aagtcaatac ttcggggaat gaaaatgaaa ttataaggtt ttctgtagca
    40561 aaaattaaaa agcaatttga agagataaaa tgatggttgg tataatataa atccctcccg
    40621 aaaatgcacg atgtttttat ttcatacagt cgcagcgatt cagtcgaagt gaattcgctt
    40681 tgccatgcaa tagaagaagc cggaataagt tattggctcg atactgccga cattccactg
    40741 gggacttcgt ttcctgccga aatttcgaga gcgattgatt cttcgaaatt gcttgtgttt
    40801 gtttcttcgg ttcattcgaa taaatcgcct tacgttacca gcgaagtgcg ttatgcactc
    40861 gaaagtggta aaatgataat accctttaaa attgattcag aaaactatag cgacgacctt
    40921 aagttgtttt tagtgacgac aaattatttt gctgcatatc cgcctccggt ttcgaaatat
    40981 atgacagagt ttgtagatag attaaaactt ctgattgatt cgaccgacaa caataggcca
    41041 gtgctaaaac gtataattga tgaaaacgat ccggatgtgc ctgctttact cgaaatatac
    41101 cataattgtt tcgaaaacga cagatgcgtt gctgatgaat ttatactgag caacctattt
    41161 gccgacaaca acactaatca taaagcgtat ttgtttgttc tgaaaaaaat gaacagaaac
    41221 attggcatgg ctgacgtaag ttatttttgc gatagcaaat tgctttttgt ttcgtacatt
    41281 gccgttcatc atttccgata tccaggtgaa aaactgatat acacccataa cattattgag
    41341 ggaatacaga attatttcca acaacagcac ctcgaaattg atgcgctact ttttgaaacg
    41401 gaagaagaac gcatttacaa attcttcaac agggttttta ataatcggtt tcacaagaaa
    41461 atttataaaa tttgtatcga ttatacccag ccgactctcg tttccgatag cgatccggga
    41521 gtagcgggag ggaataaaat acatttggtt tatatgcctt gccaagattc tggtgagcgt
    41581 acctcgataa aaaaagaaac ggtaattaca cttattaagc aggtttattt tgaaatatat
    41641 ctacatatta gagataatac gtatgaagaa cggtacaagt atctcgatga actgatccgc
    41701 gagtattgca atcatctgcc cgaagatatt cttcttgagt gggagtagtg ttgttgaaaa
    41761 caaaatattt aataaaacga caaagacacg ttttaagtta tgctttatat aaatgactgt
    41821 taatcagata aatgttttaa tttattcaaa ttacattttg tgcttcttaa ttatcataaa
    41881 aaaaacagtt aatctataat aaaataattg attaaaatac attgatattc attttgattt
    41941 ttttattttt ggaaataatt aagtgcaaat aaataaaaca taacctatgc gatttaaact
    42001 ttactttttt agcatcatac ttagtggttt tcttgcatct tataactgta tggatttata
    42061 tggtcaaaat gcacaagaat tcgtaacatg tattgttctc gataaaacgg cttcgatggt
    42121 tggcaaaccg gatggttcaa atatttgggc tgatgttcag aactataccc tgaacatgat
    42181 tgatgcaatt tcgattaaca caacagttat cgttttcgag tttgatggga aatgctatgg
    42241 cccaaaaaca tttacaataa gtcaacaagc tgacaaggga gcaatcaaga cctatgtaaa
    42301 aagtgttaaa cctgatgggc aaagcactgc gatttataac gctcttgacg tagcatggca
    42361 atatatgcaa aacaattact ccaaccataa taaaatcata tatttaataa ctgatggtcg
    42421 cgacaatgca agccaaatga gtttttcatc ggtgctatca aagttttcgg cgaataaagg
    42481 agaatatgat catttatact atatcgattt aagggatatg gccgatgcaa acattaaaaa
    42541 cctggtttcg caaaccgaat cggtaagtat aaccaaggga tttgttaaaa ctattacagt
    42601 acaacccatc tttcaggaaa taccggtgac acttgataaa tcaaataaaa ttgagcagcg
    42661 atttgcaata agtggaggtg ttttaccaga gtccttttcg ttcaattgcc aggtcgtaag
    42721 cccgaataat attaatgctg atataaatcc gtccggaaat atcggacagc aaaatcttac
    42781 caaaattgat gatggtaagt attccttcca atttggtcta gatattttac aagggcaact
    42841 ggtagattct gctgaattca aaattagcct ccatagcaat tcaattaacg agtatcttat
    42901 aacctttcaa ccggaaacat ttactattct attgaaaaac aaggatgtgg caaaagttaa
    42961 agctaaaaat gggggatgga gatagtttgt acgaatctat taacacataa gaatatgaag
    43021 aaactgaact tactgttatt catggtgatt agctttgggg gatttgcaaa caatgctatt
    43081 caatgcactg attctatcac aaggcaaatt gaactcgagt ttaataagga agcaattgaa
    43141 aacgatgctt ttataacctg ggaattaatt ggtaattggc aggagttcac cattacaatt
    43201 aatcaggggg tactcgaaga gaattctttt accgtaaagg caaaagatta caaagatttt
    43261 gctaaaggtt tcgatggcat tcgaatctgc ataaagccga acaagcatac gaaagagggg
    43321 acataccaac tctcgatgaa agtttcggat ataagcgaaa atttagaatt ttcgaaggaa
    43381 gaactggact tgataattcc ggaattctac tatcaggcac cccctccgag accattatgg
    43441 caatacctcg tttttggtct gttgattgcc tttttacttt ttaatattat ttggtttgct
    43501 ttggtgaaac ctaataaata tcgaaaaatg aatggtacat tgatttttga tgatggtgcc
    43561 gcaataaagc tgaacaattg ctataaatat tgtatttaca cagccatgac agctcctaaa
    43621 ttgacaaaaa atggattttt atcaaatctt tattttggaa aggttgggaa atatggaatt
    43681 attaatttcc cggattattt aagaaatgaa aacaccaata ttattatcaa acaagttaaa
    43741 gaccggaata aatggaaaaa cgcagtaatc agccatacaa acctgcagtt aatttctgaa
    43801 tacaagaaat tgtatcatga acaggattat gtttttcagg acagcattaa tccaaaggct
    43861 caaatttctt ttacatacga caacattaaa catcaactgt aatcaaagta ataaaaatca
    43921 acattaataa ctatgaaaac aaaactttct agaactgtat ttattggatt gggtggtacc
    43981 ggagcgcaaa ctatagtgcg tttaaaagcc aatttcatgg aaaactttgg aaagatacct
    44041 ccaatgatta aactattggc tattgatacc gacagtcgct taaagatatc ttattcaaca
    44101 acatcaggca aggagtacat gcttgacgag catgaattta aacatttaat ggttgaggat
    44161 gttgtaagct atattaataa caacccaaaa acatgtggtt tttttccaaa ggatttgata
    44221 aaagctaaaa cttcaatata taaaggcgcc ggacaatttc gtgcttttgg aaggttgcct
    44281 ttgattagag aatttgacac caattttaag gttttccttg aaaataccat taaaaatgta
    44341 aggaactgga taggggatag agattctgat tttgaggttg ataaaaccgg aacaaatgtt
    44401 tttgttgttt tttcgttggc cggaggtacc ggtgccggta cttttattga tttcccttta
    44461 atgataaaag gttcaggaat attagaaaca aaggaaaaaa ttattggggt gggtgttttg
    44521 cctgatgtgt attccagctt cggattgcac gctataaata ccaagcctaa tgcaatagct
    44581 ggtattacgg aatatgaatt tctggcgaat aatgtgatgg gagagatgct aataaatcca
    44641 aactcaccgg aattactcat aaacactcca actgcaaaat ataaggttga ttataattat
    44701 ttgtacgact catttattct tgtaaacaac tatagcacta caaatcaaca atatgataag
    44761 ccgggtgaaa ttgctgattt aatttcgaaa tcactgtttt tagttgctgg ttctgcaggc
    44821 ggagaaaatg aaagcactct tgataacatc tttacaaacg caaaagtttc agaagagagt
    44881 tacggaaaac ctagtcgcta cttgggcatt ggatgtgctg aattggtttt gaatagggaa
    44941 cgtattgctg attattgttc atttctcctt gcagggcagt tatgtcaatt gatttctcgg
    45001 gaaattggta agcaagatta tgaccatgct gttcaggata gggtttcggg atggaatata
    45061 caagaggatc aaaaccaaga cgatgttatt actaagctac taagcaataa gttaaaaaac
    45121 agattcatta atttgagtga attcgatgat ggggctgcac aaaccataaa agcaaaaagg
    45181 actgcatgga ttgagtcgtt taccgtaagt ataaaagatg aaatctattc tgatgaagga
    45241 agccttaatc aactgaaagg cgataaattg aaaaatttga ctgattatat atattcaaaa
    45301 gtatctgagc cggggggcgt tgctttttcg aataacttca tacttgtttt aaagggacga
    45361 cttttggcaa tgattgaaga aatgagaacc gagtctgccg cgttcgacaa aaagaaagaa
    45421 caagttatta ggggatatga cgaaaagtta gctgctattt cggcttctga aaaaatttcg
    45481 aagtttaatg tatttaaaaa taaatcgaga aagattgaag aagcctgtga ggactttgta
    45541 gagtcggtca acagagaggc tttatatatc tctgaaattg aaagacgtaa tgcagccgtt
    45601 tcgttctttc agtcattgat tgacagaatt aattcaatgc aaaatcaaat tgttaatttt
    45661 cagagtcgtc taagcaaaat aataaccatt atctcaaaaa acattcaaac tattaggagc
    45721 cgaaacccac aggaaccttt tacggtgcat ttgacccctc atattattca aaatatcaaa
    45781 gtgaacgcca atgaggacga tcttgaaaga tttataaaaa acaatttgcc tgcatcattt
    45841 atttttactg aacaaaccga gatagaactg ttaaatatct ttttaaaatt ctgccaaaaa
    45901 gagcatatta ctcaggaaaa taaaaaactc aacattcttg atgcactcaa tgaattagaa
    45961 aagagcaagg ttgaagaaat attggatgac ttaaaagatg ctattggtat tctgagaaca
    46021 attgataaga attatgataa aattactgtt gcgaacaaat atgtaattgg cgttagcaat
    46081 agcaatcata ttagtttgat taatcataaa aattcgcaag agtcgtttga gaagaaaaca
    46141 gaagttgcca gtaaaaccga aattcaaacc attgaaggaa gtactgtttc aaaatcaatt
    46201 cccgatgacc tagggcatat attagacaga aaatttagcg agaatgggaa agcccctatg
    46261 tttgccaata ccggcgacga aagtaaaatt attattactg catttgaatc gactgttccg
    46321 gcatttgtcg ttaataattt tagaaactat aaacaagaaa tggacaatag tacaggtatt
    46381 tcgcccattt ttcgttttgc aaataaatat tggggcgaca ttattcttaa gacaaattat
    46441 tcaatctttc cggttgatac cgacgacaca cttaaaatat ggtcactcgg attgctggtg
    46501 tcttacgttg agaaaatgca gttaataggt aaacaaaaga atcgtgatta ttttgttgca
    46561 gtgcggtcta tgggtagcaa tattactgaa ttagattctt tcaggccaaa agcatttgaa
    46621 atatttaacc aaaaacaatt aggattggaa ctaatggttg aaatcaaaga taaaataagg
    46681 aacaatttga gtacctacaa atcattaatt aatgaattaa tagaggacaa aacagataaa
    46741 aaatatattg atgaaattgc aaatcatcaa ttggataaaa agaccttaaa cagcaatagt
    46801 cacaaccaaa ctcgtgaatt aatattaaaa gaacttgatt ttattaaaag catcggaaat
    46861 ctttcggaaa taatctagtt gtgatatgtt cataaacaga accttaatta tcagttcttc
    46921 atcattgggt gaagaactga tttctcaatt ccggcgatct attttaactg attttcagag
    46981 ggagtattct acaaaacttc aaactaagat agaaacactc tttattgagt cagcaggtat
    47041 tgttaatgag caaaaacaga ccttgtttga tttgagtgaa ttgaaagatg aatcggcaac
    47101 tgaagttgtc tggaataagc tgatttcgga agaaaccaaa attttgaacg ttatttcaga
    47161 tgcaattgat tctattagta atgttcgaga cgaccttgct ctaaacgaag tgcaactcac
    47221 tgaaattgga gaatctatca atattattgt gttgctacca acttataaaa cgcgtgaatc
    47281 agtttggtta agctatttac ttaaagtaat attcccccaa ttaggcatta cgaattataa
    47341 ttacttcgta tttggggttt tgccagagtt agacaaggat tataaatcca acgaaaacga
    47401 gtatttatgt cgatgctttg cctgcctgac agaatgtaat gagattttaa atgaaaagat
    47461 ggatttgatt aattctttca cgctcttatc tgataaaaat gaggacaata ctgctgttga
    47521 aaattattcg gagttaatcc cattattgaa agaattgttc ctctttgtat tttataacga
    47581 gagtcctttc aatcataata ggcaatcacc cattccggtt tatcgggaaa aaagagcctt
    47641 ttttagtgct cttgggctta gtgctgttgt gtataaacgt gacagattgc tggaacaatt
    47701 tgtacataac attattgtca aggtttttga gcagttctta gaaattaata gaaaacccat
    47761 taatcgcgag ggtttttata tagaagcaga tcagcttatt aaaaacttag catgggagaa
    47821 aaaagaaagc ctttttgaag gccccgaagc atatcaatct ttttcgaata catataatct
    47881 ttcagccaat agcatttcaa atctatcaac agaccttatg ttgaagtcgc tgaatgctaa
    47941 taaagaggag cacgaaagtt acaatggtta ctatggaggg acatatttaa atcgagtact
    48001 tttagaaaag gataaaagtt taaacaaaac caaggaaaaa atagtagaaa caatagcgaa
    48061 ttatcttaaa gaaggctcgc aagggttttc aaaagcactt gctttattgt ttttattaat
    48121 gggaaaagaa gacgattcgg tattcgaagg gaatagccat gttgagccag cagatttact
    48181 ctatataaaa gctaaggttg cagaatatta ttgtagcttt ctgactgaaa gcgaattaat
    48241 tccaattctg gattttaaaa ataaagaaaa ggaactaaaa cgaaaaattg aacaagcgga
    48301 gaaactcagg tgtaaaattg aaaacctgga aaacaatatt tataattttc aagaagttaa
    48361 agaagatcta tcaaaacaaa caggtgaatt actttatttt actatttccg ggcaaaaagt
    48421 aaatattaca ggaataaaaa aagaagaaga acccattcct gatactatga aagcgtatat
    48481 accagaaata acggataaac cattgcctag tatcgtggat ttaagaccct atttaactcc
    48541 tgtagaaaac caattgcagg ttgggagctg tgttgcaaat gcatgtgtgg gcgcgtatga
    48601 atacttagta aaaagagcaa cttcgaagaa taaagatttt agcaggttgt ttgcatatta
    48661 taatgcacga aacacggatg ggactggaaa tgtaactgat gaaggtgcaa ttcagtcaaa
    48721 ttgtataaaa tccatgatgg aactaggtgt atgtcttgag tcaacgtggg gttataatgt
    48781 cgataaggtc aacacacgac ccgatgttaa atgttatgag gaagccacaa attatcgtgt
    48841 aattgatgca gagtatttcg gaatagatat agatttaatg aaaaaatgtc tatctgaggg
    48901 tttccccatt attatcggat tgaatctata taattcgttt caggctgttg gtaataaagg
    48961 gattgttcct cttccggatg agacggaaga gcaggcagag aaccatggaa gacatgccat
    49021 gttatgtgtc ggctattccg atgcagataa atacttcatt gttcgaaatt catggggaac
    49081 tggttggggt gatgcaggat actgttatat cccatacgaa tatatgggaa atagtaattt
    49141 tcacacaggt tccattgttt ttcttgtgaa agccttgacg gatttggatt ttaagaaaat
    49201 agattttacc aaaaatattg caaaggaaaa aggctcccta ttcaatttta gttatgatct
    49261 attagaatta caacattcaa aagaaaagta ttttgcaata aagcaggaac tggaaaactt
    49321 agtgcctgaa tttgaaaact tacagcgtag attaatagat cagcgtgagc tagtacttga
    49381 ttatcaattt agggaagaag tcgaattgaa ccaaataaaa gaattaggca aatcacaaga
    49441 ggaaataatt tcagaaattt taaataagga tcagttgtta aacactttaa atgaccgaaa
    49501 atctgagtta aaacaaaagc ggaagcgatt tattttaaga cgttttattg tagttcccat
    49561 tgtaatagtt ttgtttgctg tgcttgcata tgttgttgtt ggtaagtttt tgagtggcgt
    49621 ttggttaatt agttttgctc ttatattacc catattgttg attagtgttt tgagtgtttt
    49681 ctttattgtt tatggaattg taaattatat agcattaaag agaaaaatca ttaatgaatt
    49741 aaataaactg caagagcaaa tactgcagga agaagaaata cgacggtctt ttattgagaa
    49801 gttgattaat aattacaatc aatatttcga ggttaaaaga acgaataagt tattcgaatt
    49861 attattggat tttatttctg atgtcaacga ttatcttaat atatctaaag acaaactctt
    49921 gcaatggtta gtgattttaa agcaacaaaa agaaaaatca gaaaagtttt taactgataa
    49981 tgagatcact gataatggtg caaccatttt gcagtatgct tgtacaacaa atgatatttt
    50041 gaatttggtt aatcgagttg atacattaaa ggagttttat aatgatggta agcgcccgct
    50101 gcatgaattt gttcttgata ataatggtcg aataatgaac gacgagggaa tagatatctt
    50161 taagggtgct gccatatcct ttgcgcaagg gaagacagaa tatgaattaa tgaagggttt
    50221 taatatttta gattttgttt ttaaatcgga gtttataagc aaagttttta aaacaatcga
    50281 catgacacat gatctgaaaa ccaacgtaaa aatgtatgca ttatactcaa agccatttat
    50341 taaggttaca tctaaatacg acacaataag tattgatcat agtattgaat tattatggcc
    50401 agatattgaa gaccattctt ttgataaaat tagggatatt gttgaatcta tttatgcaat
    50461 taaaccgaaa attttcaagt attcaaacag tacagaatct gcattaatat tcattcaaat
    50521 tttgacattt tttccggcat atgctgttga atcgcttgaa aaagctgcca gagtattttt
    50581 gaaattacca cctgcagaac gtaataaata ttttatcaaa cccaaatttg gtgcacaatc
    50641 atatatgcct gaattggaca atgaattgca ttagcgtaag taataaaatt tctaaatatg
    50701 agtaatttag ttaaaatagt gatgataata attgtggcgt ttggattttc atcctgcacc
    50761 aatagcacga aaacaaagaa aattgagaag ttccatgctt tcatcgagga ggttggtcaa
    50821 aatcatgaat catacacccg tgaagactgg acaaaagccg attcaacatg caaggagttt
    50881 accgattact tcgatcaacc tgaaataaaa aagattctta cccaggagga ggcagaagaa
    50941 attgcaaaac ttaaaggcaa ttataaaggc tatcggttta agccaaagct cgatgaaacc
    51001 atagatgatg ttatcgacaa agcatcggaa gtgggaaaag aactggaagg agtgattgaa
    51061 acaataaaaa aatagcaaat actacatgga taatatcttt acattcagca attttcttga
    51121 atcaatcgag aaaaaaaatc ctgagttgta tgcccactta atggggaagt acacaaaaaa
    51181 aatcagactt accgatattc ctgaaataaa ggaattggtc gaagaatttg attgtacaaa
    51241 tctatatttt ggaagtttgc cgatatcaat cgacgacaca ttactccttg tacgcttaat
    51301 tgctgcctct ataagctgcg attacgaatt gagttacgat aacgaagtgc accatgaatt
    51361 gcttacgatt acggttgggc ttggagtaga aactgtgaat agtgaattga gcgaactgct
    51421 taaaagccaa ctcgaacagt tatttattat attgttatcg caacaactcg agttagctga
    51481 tgaaataaaa cttgaacgaa gaaatcgtct tgcagattgg gaaataaaaa gagcaatgaa
    51541 caccagcaaa cgttattata atgtgttttt tgaaagaaat tacgattagt tcagctatat
    51601 catttcataa ttacggagtc atttaatacc aggttataaa aacaaattca gaaaagatga
    51661 gactatatta cggattaaaa tacagatatg ataacatcaa tgaactccgc ttttcggagc
    51721 tggcccgatt actattttat tcgatgagca attcagtaac agatgttcag cgctttatat
    51781 ccaaacttaa taaattcgat ccggcaaacc ttgaaactta ttgctcggga ttagatctgg
    51841 aggtttatac aattaaggaa aacctacgat attctgcgca cttcacaaat cgaatggtgg
    51901 aacggttgca gaattggatc gacaatatag gttgtacgcg gcaggagatt gaggatttta
    51961 tcagttacta taaaattcgg tcagataaac tcgtagggct tcggatgatc agtaaatcgc
    52021 aatacaacag tcctgcggat attaatacta tgcttggcca atatattatt ggacaagaaa
    52081 gagcacaaaa aattctcagt tcagccatct acattcattt gcttcgctgt ggtctggtaa
    52141 agccgcttat atcggccggg tacactactc agaattcagg ccttgttcca ctgcttccca
    52201 accccaatat tgtgctgatt gggtcaaccg gtaccggtaa aacctatatt ctaaagacta
    52261 tagccagcgt tttccgtctg ccttttctta aagtggattg cgcatcgctt acttctaccg
    52321 ggtattacgg aaacggtctg aatgagcatt tatatacatt cctgaataaa tgtcaggaaa
    52381 gtaagcagga cattaatcaa gccattattt tctttgatga atttgacaag cttagcgaaa
    52441 tgcattatgc ccgcagtggc ggatcgttgg gtggcgttga aatgcaacag gaatttctga
    52501 atataatcga ggacaaagaa atattgttat acccacccaa gggatcgcag aacgaaggaa
    52561 aatcgctcaa cctgagtaat tgcatgtttg tttttggggg gagcttctac ggaatcgaac
    52621 gaattgttga aaagcgcctt ggaaaacatc aaaggatcgg ttttaaaatg catgaaaaaa
    52681 atactaatga tgatgctcct gtattgagtc atgtcaaccc tcacgatttg gttgaatttg
    52741 gaattttgcc cgaactggtg ggtagaatta atcatgttgt cagtctcgaa gaacataacc
    52801 tcaatacaat aataagtata ataaagaatt ctgccgaatc gccgcttaaa gcgtacgaaa
    52861 actatttcga gatgcaccac gacacactgg ttatcgaaga agatgtatat gagttaatag
    52921 cggaaaaaat tctagaggcg aacacgggag caagggcaat atacggtgtg ctgaacgagt
    52981 tgcttaagga cctcctgtat cattcaccca atttaatcga agaaaaactg gtcgttgata
    53041 aagcgtattt taataaggtg ttcccaaaat aagatgtatt tttgaaaaat aactaaaacg
    53101 cattttgttt acacaattca ttattaacgg cttaatcacg cgaagcatct atgctcttat
    53161 agcccttggt ttttcgctcg tgatcactaa acaacttcat ttttcaaatg tgcaaagggt
    53221 tatttaaaca acaaatctat gaacccaaac gaaataaaaa tcatcgacga ttcaggcgtg
    53281 gagcagctaa ttgattttga attacgacaa tgttcccaaa tcgttccttt cagagaagta
    53341 aaagcgcaga taaaaaaaat gtttctcgat tgttataacc acgtggaaac attatttggc
    53401 gaccatgcgc cccggctaat aaccgtaaga ttggagcagg aaaaaacaaa tttgcagtca
    53461 gtaattatgg cgtcgttccg ccccaccatc tccactgtga tggaactgaa gtttagtttt
    53521 cattatcaca ctgttcaact tgtttgtgcc gatttgaatg ataaaactga tgagaatacc
    53581 ggaaaaatta aaaggattat tctgcatgaa ctcattcatg ctgctgactt aaagaattta
    53641 aatcaatttc acttatctca cacagcaaaa tcagcacaac ccgggaatat tccttcacaa
    53701 atgatatttg gggaaacatt cggtagcggt cagtataatt atacaagcca ttacctaatg
    53761 ttacatttta ttcaaaaatt tcgtgccgaa ggggttgcta tattgggcga aagactgttg
    53821 aaccaacata aatacagcca agagattggg gaatttataa aatactttgc ttccgatttc
    53881 cactatgtta ttggaaagtc ccgtgatata caatttttcg aaaagaatat cgataaggta
    53941 actgtagaaa acctcaatat tatatgtgaa cgagcctaca tgtatggtga tgccgttatg
    54001 tttagcctga ttgcacaaaa acatggtaaa gatttcaagt ttttggattt gtcaagaggt
    54061 ttcgataatt tcattatccc tgtgtttact tattccgatg ctattgagtt tatgaaatta
    54121 tgtatcaatt tcgatatgtc cgattacatt aatggtatgc ttcgtttgac ggataacaat
    54181 gggaatacct tgattgacca caataagttc tataattatt gtggtttcat tcaaaacgaa
    54241 acaacgggga gcattaacga attttgtctg aatatttcga aaaccgcgtt gcattacaac
    54301 aaggaagcgt ttatttccac attaaaagat gtgctgggaa gtaaaatgca aaaagaagag
    54361 attatagaat tgatagctga atttgaaaac acaacacctg ccgataacat ctcgaaagat
    54421 ataaaagttc ttgcatcgca gttggttgaa aaaattaaca ctgaagattc cgaaatcgtg
    54481 atatgggcat taacttatct gctcgacgat gaagatatga ttcacgatga acttgatttt
    54541 gtcgggctcc aggatgattg gtttgttttg aaagcagccg cttatataat tagtggaaac
    54601 gaaaaatcga atcagttgta agtagtggca aatggcatct ctacaaatcg cttgtattcc
    54661 agcagtgctt caatgcgctt tttaccttcg atagtgtttg ggtcgtatgt atgaatcaat
    54721 gcctctattt ttacaattat tcctgacttg tccaatttag tgcgtttgag acaaactttt
    54781 tacctgtgac aactaaagtg attagatcat ctactcattt tttctcatac tttttccttg
    54841 atgaaaaagt aacaaaaaat catgacaaaa acatgcttct cccctctcta gccgtttaat
    54901 gaaaaaacta acaaagtagt gttttgtcag cacaggccct tctccttagg ccgacaaacc
    54961 aaagcgtcga gaagcaaacc agggtcgggt ctgcgggtag ttcgcttctc tagccctttg
    55021 ttgttttttc tattaaacgg ctattcacgc ccgggccggc tcgctttttt gtcgggcctg
    55081 cccgcgtcgc gttgccagca gtggactgat agcttgggtt gtctgtggaa atggcaatgc
    55141 gattgacaac acagattttc atgcatatcg taacgccgag gggggagtga acgagtgcgc
    55201 gggaagcagt atgagtattc cataaggctg gatgtgcgat acttcggctc cgctcagcat
    55261 cccagggtaa tgctgctttc ttgctcgttg tgcccttttg gggttgcttt tttggagctg
    55321 tgcttagcga tgccgaaaca caaactaaaa agtgataaaa aatttggtta gtgcggtaag
    55381 tataataata tttaatcaac tcacgttgat tatcagtcat ttataacctc actaatttat
    55441 tgtgggacaa gtcagaatct caacaaatcg cttgtattcc agcagtgctt caatgcgctt
    55501 tttaccttcg atggtgtttg ggtcgcatgt atgaatcaat gcgtcgattt ttacaattat
    55561 tgaattggta aaggttttgt ctggctgtag aatttcgcgt atggcaaatt gatatagctc
    55621 attcatataa aatgacgaag tatcgcggtc gaattttaac cagcctttaa tagaatgcaa
    55681 taaatcagtt attctgttat tccccaatgg actaattaat ttcagatctt cggtagcgat
    55741 atattgcgat ggcaaaacag caaaaaccat aagcattttt ttctggtcat cgttcaagtg
    55801 aattaagtcg cgacaaaaca gtgctttaat atagcacatg ggagtgcatt cttcgggaga
    55861 aagtgctaca ggtaaattgt cttttgtgcg agttataacc ggtttttgca gtctgttgtg
    55921 cttaatcgac gacacccctt taataatctg gtaaatatcg cataagtcaa gtaattcgtc
    55981 tgcaatatca acacgggcaa tcaattcgat tagcaaggga ttaaaggaaa gatttttcaa
    56041 caatgtgtgg ataccctgat aattgctttc aatgaattgc aaggcttgtt gtttttctcc
    56101 ggcatcaatg tcctcgagat aatacgattt taacatgtcg taagcatcgc cggggtcagg
    56161 cttctccagc cgaaccggta tcggtgtggc aatacgtgaa ctgctgataa atattacctt
    56221 ccatgcaata ttcagccctt cgaaacttcc gataaaatca agtatttgag ctgcgctttc
    56281 ggcattgtca ataatcatca agttattccc actatattgc agcagtattt ctgaagcaga
    56341 tgtaaagcat tgatacagtt ctgcctcgtt attaatttcc ggtgcaataa attggggaat
    56401 ggcaaccgac cggctcatgg cttcgatcaa cgacagtttt atcgactttg taacatttat
    56461 ccacatcatg tgatcgaaat tcgatgcttt cagctttaca tagtattgca caagactggt
    56521 tttcccaatg cctttaattc cattgataat tgtgtacttg ttgctatcga tatgcagtga
    56581 taaagtgtct aaatatcgtt ttcgcccgat aaaactttcc ggtttttgcc agtagaacaa
    56641 tggggtaagc gcgatagttt gctttttaat tggctgcggc tcctgcaatg caatatgatt
    56701 gctgtttttg tccgttaatg tctcgctcag cagtttaacc gtattggtaa gtggttgatc
    56761 tttgtaaagc ctgataaaat ctagggcaag gagtttaagc aaccgggtta tatcacgatc
    56821 gtcgggttcg ggttgtattg taatccccga caataaattc aagttcacat aatagcaaac
    56881 acgacgaaaa tactgatcgg tattataggt ttgcttcaca tgggtgaggt tcagtatttc
    56941 ggaactgatg gcagcatttc ccgattggtt caaagggttc ccctcacggt attcgtagta
    57001 tttcctcaga ttgtcgcgaa gcgtatcgtc aagcagcagg aacttgttaa acaatctgct
    57061 gatctgggca ggtgtgattt ttattttgga catctacgaa ttcgaatttt ccggtaatct
    57121 ctgtaatctc tgaaataaat acgattgtta taacaaatct tgcacttttg taaaaacaca
    57181 aaagcaaaaa taatgaaaac ttcagcacgt acacacaatt tcaccatgtt tgttttaata
    57241 tcggggacct tagtgctatc aggctgctgc aagcagaaga gcactcccga aaattacaaa
    57301 ccaccgatat tttccgaaaa tggaaggctt tacatgaacg caaaccctga agttgatatg
    57361 ttctcagtcc gggcagatag tgtatttggg ctatattcaa acgattctat cggtcttttt
    57421 ataaaagaca aacgctatgg ttatctgaac gtgaacaatt cgggggtagt tatccctgca
    57481 aaatatgatc ttgcctggca atttaacgat tcgggtagcg ttgctgctgt ggtagcagga
    57541 ggcaagatgg gttttattgg cagaaacgga aattacttgg tagaaccacg tttcgatttc
    57601 gatacagcct attttaatga ttatgatttc acatacagcg atgactattg ttacatcccc
    57661 gggcaaaagg gcaaattcgg aataattaac cgtcgctttc aggtagttgt ttcaccgatt
    57721 tacgacaata ttgaaatcga tggcaattat tttattatct gggacgcagg gaaagccgga
    57781 attgccgata cttcaggcaa tatcattctt aaacccgaat tcgataatat cgatgtcatt
    57841 cgcatgggtt ttgtgattta taatgaaccc aaaaatgaaa aatatgtcct tgacttcgat
    57901 ggcataacaa cggttgcttc tggcgttttc gattttgttt actctatccg gagtagtcgg
    57961 agtcgttccg acgagtatta cgatgacgaa aatatatatg ttgctgatag cattcccgta
    58021 ttctatagcc tcaattatgc aaaattcgaa atcagtagcc tacagggggt tattcacagg
    58081 cagtcgggca agatagtttg ccccgcaaaa tgggacgatg tatttatgtt gtcggaagat
    58141 gtgctggttg gggtttgcga tgattattat tttatcattg acattaagta attgaaatta
    58201 ccatggaaaa aagaacaaaa tattttggac tggaataccc aacccagata catttacctg
    58261 aagatgtaaa gtacacattg gcaaaacttc tgtttgcgag tccaacggca gggatcgaag
    58321 aggtaaagtt ggtgcttgaa aacgaaaaac tagtttcagc agttgaaaat ctgatatttg
    58381 ccctcgattt tgaaattgtt tctgttgggc agcctgatga agatggacta gaatttatga
    58441 gcaggaaaga aacagagaaa ctgatgaaaa agaaaataaa aaaatggatc tcacattttg
    58501 ggagccctac cgaaaatgaa atacttgatc tggcgaatga taaaaaaatt cataataaag
    58561 aattctggct agaggtatat gaaaaaatga aacgggttaa aatcaataca attcccgaaa
    58621 tagagagcta tctgaagcag tacgtaaaag gtcaggatga agctgtacgc accatcgcaa
    58681 tgatggtttt tgagcatcgg cttcgcttgt cggagcatat tgacctgccc aacaacgctg
    58741 tttgcatgat aggcagtacg ggaacaggaa aaacctggct tgcctcaaaa gcaggtgagc
    58801 tgcttaatgt taaaccttat cgtatcaatt gtgctgaatt ggtgccttcc ggaatcgtgg
    58861 ggatgtcgct tgagaaacac ctcactcaga tgtatatgct ttacaatggt aataataaga
    58921 aaatgtccag aagcatgatc cattttgacg aaatcgacaa aatttcgtac cgatatcata
    58981 tatccgatgc gtttaaagca acactgcagc tcgaaatgct tcgatttttc aataaaggag
    59041 aaaaactgaa tttcaatacc agaagctatt atttatctga atcgaccaat attgaaaccg
    59101 gttcgatgtt gcttgtgttt tcgggagcgt tcaatggaat tgaggaaata attacacagc
    59161 ggctgattca ggaaaacgat ggcaaattat ttttggtcga taccgacaat atgattcaat
    59221 attgtaaaac cgatgacctg gttaattatg gatttattcc tgaacttgcc gccagattga
    59281 acaatatttg tccgttgcag cctcttacaa cgactggtat tagagaaata cttagcaatg
    59341 ctcaggaatc ggagttaagt aggcatatga ataaatgcaa agcattgaac cttaaagtta
    59401 ggttttcgga tgatgccatt gcactaatgg cagaaaatat tgtaaaaaag aaaattggtg
    59461 ccaggggcat tgctgaagtg cttaccccct tgctttcgca ttattatttt aatgccgatc
    59521 aatataaagg caaagaaatt gttatcgatg gcaatgctgt gagacgaatt aattttcatc
    59581 gcaattacaa aaccttggcc aaggcttttg aacaaaaaac gccgctgaac gaaatcatgg
    59641 agcagttcaa agcaagtgcc gatgaggtac ttgacttgta tctgaaatgg aaagagataa
    59701 attaaaacct aaatagaata acttatgtta cgatttgatt attacgatta ttacagaaca
    59761 ttatcggaat acgaatactc agaagggcgt gaaatattcg aaatctggaa acaggcgggc
    59821 ggaagttttg acccagccaa cgacaaagta atggactacc tgaattgctt taccccgata
    59881 ccgtttaggc agccatcgtt tgattttgac cagaaaacaa ttgacttgtt atttaaactt
    59941 gtggttgcat caaccagttg tacatacttg tttgatttga aagaaagcaa aatcccggaa
    60001 ttggtaatat ttaatacgaa tatttgcttg ggaaatgaga gttttaagct atcggaatgt
    60061 accgaagata ttatagaagt acttatattt gttttgctac atgaaattgc cttgatatta
    60121 gcatcgaaat acgatgatgt catggaagat aaaataaagc aatataagga aaaatgtatg
    60181 aaattacttc tccctaagta tactaactca aacaatcagg caattcaact caaaagagga
    60241 tttcagttta attactctga gtttattgaa agtattgaac gaagcgataa tgcagataat
    60301 gtaaaactct atcgtatgtg gattgccaac catggttcaa aaaacataga gaaagaaaaa
    60361 tttcacagtt atctcaacga gtttgagtat ttccccttta gtgttcctcc tgatttgatt
    60421 tcttctttcg attgggaatt atttttaaaa ctggtagccg gttcaacatc gatcagttat
    60481 gattttgtgt ttgaaccctc aatagcccga aatgagacag aagtttatcc tgaattatat
    60541 ctttatttgg agggtgaatc cgaaaatata tgtaaaaagg tgtcggaact tgaaccgaat
    60601 caggttttca ggcttttcga aatatattgt atggagttta tccaatttca atatatgggg
    60661 agccttttca tgccaaattc aacattgtat aggaatgatt ttaagaagag gatcgaactc
    60721 tataaacaaa agcgcaacga agcgcaaatg attgctgatc gcaggagctg tttttttgag
    60781 taaaaacttt tcgaattagt tgaaaatggc atttatgcta gaagtaagtc aatacatcat
    60841 aatcgtaagg gttttcaact acatgcatat tattgctgat cattttagta ataaaagctg
    60901 ttttcaagaa ataatgatca taaatacatg gaacgaaatc gttacgggta atggataatt
    60961 cccttttaac aagattatct gtttcgtcaa agatataggc ttcttcgcaa tcaataacat
    61021 tacactccat cttaaatagt cgaatgatat catctgtatt gatttttatt attccatgca
    61081 gctcctcttt ttgcaatttg tacttattga taggaaaatt gctctgaaat aataatacat
    61141 cctgaacttc acagttttta atattttttt cttcttggaa aaaataaaat ctgtgctttc
    61201 caaggtttat aacattttgc aattcgattt taagtcctat ttcttcttca acctcgcgta
    61261 acccatcctg caattcttct ccaggtaggt aatgacctgc tgcagctgta tcaatcattc
    61321 taggaaacgt atctttatta gagttacgta tttggaaaac aaggctggtt ttattatttt
    61381 ctttacaata aatatggaaa tgaattgcac catgccagtt cccatcccgg tgaacaaggt
    61441 ctcttggttt aatgaaaccc gatttctctt ttgttttact atcgagtatt tcgaaaaatt
    61501 cattttttat tggataatct aatttagatt gaatttgcag gaagtattca tttaatattt
    61561 ttttttgttg taatgaacat tttccaatca attcatcatt gacacctaaa acattataag
    61621 ttttcatagt ataccttgtg tttaagtaag acatatttta aaaaatatca ttttgaactt
    61681 ttgtggtata taagtaatgg tatcaacaag atattttact aagtgaaaac actttaacca
    61741 gtttattgat acctgtatga taattcaaat gaaattcatt ataatattac ttcaatttgt
    61801 aacactcaat tcctgagtcc tctttttcta cgctgatctc acaacaagcg ctagcgagct
    61861 aatgcctgtg caaaaatcat ttgatattcg ccatcgcctt tttcaactaa attcagcgaa
    61921 acaccgccaa ccaattgcca tccctctgcc aaagcatgag caacgagttg ttcaaattca
    61981 ggtgttacat aatgcgtaat tactttatat tccttcataa tgcttttgtt taattcatat
    62041 tttataccgt aacacttttt aaatcaaacc aagaacttaa aaaaaaacga attacaattt
    62101 taactcattc ccaatgggat taacaaattt gcaatacaca tcaaatctac aaaatatatt
    62161 gcaatgtgaa tcaattattc taattttgaa tagaactaaa aactgatagg cctacacagt
    62221 atgttgcaca atgaactgat ttcgcacaga cgctatactg actttttaaa gtgcagcctg
    62281 aagctgctgc ctcaaaatgg gatgcctcaa gccaaaattg caacgcatgt ttttgaagct
    62341 gcccgtgctg aacgcaggtt cctgaaaacg ctgttcaata ccttaaattc gcagactgtg
    62401 tgcgaatatg ttcccatgta taaagatata caacagcggc atgcccaaac tattgatgca
    62461 attaaacgtg gttgcgatta tatctttaat gcattttttg tttgcgacaa caaagttgca
    62521 tcaatcaatc tattgcgaaa agtggctgag gaatgcagca attcagtttg ttaccaggta
    62581 gtactggtaa ccgaagccga tgaaattaca cgcgaccatt tggttgaact gcactggaac
    62641 tacagcattg catccgactt tattacacaa ccattgcaaa gtgcaggatt cttgaatcgt
    62701 cggctaagcc ttcaacttgt tgatctgggc gaatctcttg gattttataa taagctgata
    62761 accgatgccg atgcatcatt ttcagcttac gaaaacaata acaaccttat atgtactgaa
    62821 ctcagcccat ggtgctattc atgtataaaa cgctacatct gtattccaac ccttataaag
    62881 caaagacctc ccagcatact ctctgcggtt accccttcgc gcagcaggtt tttgagagaa
    62941 aataatattc acaatatcga tatgctcaat gcattaagcg atgatgctct gatggcgtta
    63001 aattttgatg tattcgatat cagaaatatc agggagcaat gtcaacgtat aagcgaaaga
    63061 aaacctgtgt tcagagtgca tttaaaaaag gactatttcg gaaatgttgt ttttgtaagc
    63121 ttacagttgg cgcggcaaaa tgaggtgttg ttcccgcgta agattttgtt cagccatggt
    63181 gcagaagcat tcgaaattac tttacattac gatgctgata caaaaagcat aggagttgat
    63241 gctgatgaag tggacagatt caatagtttg tttaaggaga aaattgtgac tcatggtctc
    63301 gatgctttgt ggctgaaacg cctatcaacg gctcacggta tttataatac acacattatc
    63361 gatctggctg attttgttgc tcaatttgtt catatgccgt tgttgggact tgaactgaac
    63421 gaactgaaat atctgttgaa tcccggagat atggttctcc atagtattgg caatatccga
    63481 ggtgctattt tatcgccata cgaaaggttg attttggtga aagaactggt taattatttt
    63541 gtgaaataca tgtattagtg gataactacg aacaaatagt gcagca
//
LOCUS       DAOOQP010000003        63000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_495_length_63000_cov_6.238780, whole genome shotgun sequence.
ACCESSION   DAOOQP010000003 DAOOQP010000000
VERSION     DAOOQP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 63000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63000
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_63000_cov_6.238780"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLK75_00545"
     CDS             <1..539
                     /locus_tag="PLK75_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY37762.1"
                     /translation="NGMEFLEILRKTPADIVLMDIEMPVMNGIEATEKAISQFPGLKI
                     IALTMFTDDDYIQSMMDAGVKGFLIKNVNKETLDRAIKTVYMGGNYYSEELFNFFTKQ
                     ISKEEPVSKTDFKLTRREKEILQLLAEGLSNKQIADKLFVSERTIVGHKSNLLLKTGC
                     KSTISLLSYAIKNKLVVI"
     gene            725..1309
                     /locus_tag="PLK75_00550"
     CDS             725..1309
                     /locus_tag="PLK75_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HOY37763.1"
                     /translation="MKILVLLMVVLCECISSAIAQTNFYDQLADSAYVLTKQNVRYDP
                     SYVVIAYPNGDVPANKGVCTDVIVRAYRKLGIDLQKEVHVDMVANFDQYPKIWGLSKP
                     DPNIDHRRVQNLMTFFSRKGTVLKNSIVPEDYSPGDIVCWNLDGKATHIGIVSKRRSI
                     DGKRNLIIHNIGEGQILEDCLFKWKIIGHYRYKK"
     gene            1764..2261
                     /locus_tag="PLK75_00555"
     CDS             1764..2261
                     /locus_tag="PLK75_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY37764.1"
                     /translation="MKTTKKVLIVDDDIDVITVLETILKKEGYTVISANDKVEGKKLA
                     LAERPDLAILDVMMTTHYEGFELAKDLIEDPKLAGMKVLMQSSIDLITTTNSSVQAMA
                     REYRKDPSYRELQVLLIKDILSNNAGVDYRAENGESIWLPVDGFLRKPVDAARLLPEI
                     NRVLS"
     gene            2365..2835
                     /gene="nuoE"
                     /locus_tag="PLK75_00560"
     CDS             2365..2835
                     /gene="nuoE"
                     /locus_tag="PLK75_00560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOY37765.1"
                     /translation="MKKNVEAILNEHPNAGRDSLIPILQEIQNEEGYISENAVIKVGE
                     YLQIPSSKIFGVATFYNQFRFRPLGKFHIQVCRGTACHVLGSKTVLEELEKKLKIKAG
                     QTTSDGKFSIEVVACIGACGLAPVISINGEFYAKVTTKAINDIIDSYKNQEASI"
     gene            2832..4784
                     /locus_tag="PLK75_00565"
     CDS             2832..4784
                     /locus_tag="PLK75_00565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOY37766.1"
                     /translation="MTATKFVNKDFLTKEVLKKFDKGQPSAETAKRLATIRNEKNQKP
                     VIFIGAGTCGLGAGAGKTIVATKKYLLNTGIDADIIETGCIGLCAAEPLLDVQLPGFN
                     RISFRNVTEDKVESILNSVFSKKLPSEPALVQFRSEGSNVWDNVSFMEDHPFFGPQIR
                     LVLKNCGIINPESIEEYIARGGYTGFLNIIHHYKPVEVCDLIEKSGLRGRGGGGFPTG
                     KKWRFAFNTEADQKYLICNADEGDPGAFMDRAVIEGDPHRLIEGMAVAAYAIGATKAY
                     VYIRAEYPLAIKRLQIALQDARAFGLLGKNIFGTGIDFDMKLKMGAGAFVCGEETALI
                     ASIEGKRGMPSPRPPFPAVSGLFRKPTIINNVETLSNIPEIISRGADWFASIGTTTSK
                     GTKVFALSGKVALTGLVEIVMGTSIRDIIFTIGGGIKNNKKFKAVQIGGPSGGCITEQ
                     NLDIQIDYESLINAGAMMGSGGLVVMDEDNCMVDVAKFFMDFIQRESCGKCIPCREGT
                     KRMLEILESITHKPTNSNQYDALERFKGILQLERLGKVIKDTSLCGLGQTAPNPVLST
                     LKWFREEYEAHIFERKCPSGVCTELRTFRIDVEKCTGCTVCAKKCPTNAIIGTAKSPH
                     FIIDSKCIGCGSCEQVCKFGAVILND"
     gene            4804..6528
                     /locus_tag="PLK75_00570"
     CDS             4804..6528
                     /locus_tag="PLK75_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOY37767.1"
                     /translation="MDLRIEVNKQVLEANKGETILDVLNRNGIKIPTLCHMKEAFPTG
                     ACRMCVVENEKTGKLITACSYPVEEGMKIKTHSSRVIEARKTIVELLLSNHPDDCLYC
                     VRNQNCELQNLSEELRVRERKIRGVKNNCYIDNSSASIVRDPDKCILCGRCVFVCSQV
                     IGVSAIDFVNRGSKSVIGTAFNRGLNTSSCVNCGQCIMVCPTGALTEKNHFGEIMEAL
                     KNPEKTVVVQYAPAITVSIAESFGMKVGKDLNGKMNAALRKIGFDKVFDTSFSADLTI
                     MEESAELIDRISNGGVLPMITSCCPAWIKYAEEFASDFLPNLSSCKSPQQMMGAVIKS
                     HYAKTAGIAPENIYSVSIMPCTAKKFEAQREEMTNKGITDVDAVLTTREFVQLIKMFG
                     IDMDKIEPESTDSPLGARTTAGKIFGATGGVMEAAIRTAYFKLTGKELLTFKVPEVRG
                     FKDRKEATLKIGDLDVTVAVVNGLANAAVLLDEIRNGRKDVHFIEIMACPGGCIGGGG
                     QKIGSSVEEKMAKMKALYDIDEHEAIKVSHKNPEVIELYKEFLGEPLGHKSHELLHTK
                     YSKRDVLK"
     gene            6556..8547
                     /locus_tag="PLK75_00575"
     CDS             6556..8547
                     /locus_tag="PLK75_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOY37768.1"
                     /translation="MSHPRRNKLVFTVKNRCRVCYTCVRECPAKAIKIVNGQAEVISE
                     RCIGCGNCVKVCSQNAKVFLDTKKEVYALIQSSKKTVACIAPSFPAEFTEINDYRIIV
                     GMIKALGFDKVVEVSFGADLIANEYAKIIDNSKSTCISSDCPAIVYYIEHYYPHLVTS
                     LAPLVSPMVATARVVKKEYGDVNVVFIGPCIAKKAESYEIDEAITFKELRELFLENNI
                     SSSSVLPVNFDPPLGGKGAIFPVSSGLLQTANKNEDLTSGKIIVASGSKSFRELIDEY
                     DEGLIRNSHLELLCCDGCIMGPGMSKQGKKYSRRECISNYVKNKLTELDTAEWEKNIE
                     KHSHLDLSKAFAPLDRRNGKPSEESISNVLKTMGKVDPNDFLNCGACGYNTCREHAIA
                     IIEGLAENEMCLPYSISKLHKYIQELNISNSKLKNAQRALKQSEKLANMGQLSAGIAH
                     ELNNPLGIITMYSNILKDESAGDDPIRKDLELIVEQADRCKKIVSGLLNFARQNQVNL
                     SETNIETLCKHSFESIVNTGNIVFEFESNLKNPMAMIDADQMMQVLTNLEKNAIEAMP
                     KGGTLKITLNGDQNSFEINISDTGIGIEKSNIDKIFTPFFTTKPLGKGTGLGLPLIYG
                     IVKMHRGQIEMTSNADPGKGPTGTNFKIVLPRLINNKQK"
     gene            8554..8949
                     /locus_tag="PLK75_00580"
     CDS             8554..8949
                     /locus_tag="PLK75_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY37769.1"
                     /translation="MTNKMKTIAIVDDDIDYLTILKMQVERMGFNVVFAENQKDGEKL
                     LETTKPDLAIFDLMMENQDSGFILSYKLKKMYPDVPVIIATAVTAATGIEFGIETQED
                     RSWIKADKYLEKGLRPDQLHREINKLLKL"
     gene            8962..10080
                     /locus_tag="PLK75_00585"
     CDS             8962..10080
                     /locus_tag="PLK75_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY37770.1"
                     /translation="METLKVLVVDDEPGIRSGIERILRNYTVGFPFMESDFSFELIEV
                     ETGEKAMETLAKDRIDIILLDNKLPGIQGIEVLENLNKMQYDAVVMMITSYASLDLAV
                     KATNNGAYNFVPKPFTPQELKSAMENITKHLYLKRMTRKMNDAGKEIRFQFLSVLSHE
                     LKSPINAIEGYLKIMQEKQVGDNIDDYQVMIDRSITRIKGMRNLIMDLLDLTKLESGK
                     KSREIKPTDMYELAKLTMQSIEPLAIQRNVQIQFDADENTYFDADADEIEIIFNNLLS
                     NAVKYNKEGGRVYFTIKALKDEIKISVQDTGIGMNDEELSKIFHDFVRIKNKKTIDIT
                     GSGLGLSIMKKMVDLYGGNIDVTSVPDEGSTFTVTLPR"
     gene            10206..11012
                     /locus_tag="PLK75_00590"
     CDS             10206..11012
                     /locus_tag="PLK75_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOY37771.1"
                     /translation="MELFVRKYGNAPQHLIILHGMYGSGDNWTSIARNLSDGFTVHLP
                     DLRNHGRSPHSPDHTYELLAEDVYEFVTGQRIERFYLAGHSMGGKTAMFFARQHPEML
                     IKMAAIDISPRTYHQLNQLSEQTIFHLNLISYLIRCNVEKAHNYAEAASILSEGVPSQ
                     RMVQSLLKNLEKTGDGMMWRINIEALKDNIYAMGDGLDPDDFIDRKIAVPTLFLRGLK
                     SNYLPVEDEKLIHFIFSNARIVNVPEASHWMHTEQPEAIVTELRHFFSED"
     gene            11084..12181
                     /locus_tag="PLK75_00595"
     CDS             11084..12181
                     /locus_tag="PLK75_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOY37772.1"
                     /translation="MILHSMLKIKLTLLIIAGFLLTVNTTFSQSASDTAIVYFTKEIS
                     PESVMKIFEYVKDNVKGKVGIKVHFGEDGNEYFVPASMVEPLCKAYNATLIETNVAYT
                     GRRQKTETHIELAKDHGFTFAPIDILDEGGTLSLPIEGGRHFKTALIGKNFENYEIII
                     YFTHFKGHGRAGFGGCIKNASMGMATPEGKRDMHFNDFPKPESKNCMGCGACVSECPA
                     NAITLNPLTIDKEKCIGCGKCISTCPYGAITKPDNAEQTIRFLEGLVEYAKPVIEHTN
                     SIFFNIIINVSPSCDCSRNPKQPFVADIGILVSTDIVAIEAAAHDLVDKAHNCDDAFL
                     KANSVSGKHQIDYAEQLGMGTKNYKLVNIDE"
     gene            12725..14296
                     /locus_tag="PLK75_00600"
     CDS             12725..14296
                     /locus_tag="PLK75_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37773.1"
                     /translation="MKKAIFLILTILSITVYAQETIKFNYNYPLEWTYNCYSMVVQNN
                     YYYLSGTIGITTDECDSGIIGYHINKINDQGIRDTIVIYSKCHHKNYLGWQGSMVNYN
                     DTIIVVGLKYIEANLNKVFIVKLNPDLDTIADFIFMDDTLTKRPLSITVDDEGNYIFC
                     GQTDSSYNEIANPAPDETYTKSYLYKVSPSGETIWKKSYPITNCSSGCWSFFRKVIST
                     YDKGYIAIGTSHVNVHKNIIVKADSLGNQEWVRFYGNPNYENPYLMDIVETADSCIVV
                     CGAYAYDEIGGGLYPYDGWLIKLDKDGNEKWNRKYRDHVLTGMSDWRDTIYSFYMGIY
                     ERENGDLITISATHSDENGVYIADKFRLRCLDSGGNIRWDRVIDSVGGQTGILQAQSV
                     KPTADGGIAVAGSAEIFYMDGTQWTSDQRIFLIKTDSLGNDVETMVKPVAAQPISKFE
                     LVCYPNPASSEFWVDLPEDDNGDVLEIYATNGSLVYEQPATPGLNRIDLCGVKPGMFL
                     VKLRNANVFGKVVVE"
     gene            complement(14635..17250)
                     /gene="mutS"
                     /locus_tag="PLK75_00605"
     CDS             complement(14635..17250)
                     /gene="mutS"
                     /locus_tag="PLK75_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOY37774.1"
                     /translation="MSAPVKNDETPLMKQYYAIKAKHPDAILLFRVGDFYETFEDDAR
                     RSSAILGITLTKRANGAASYVDLAGFPYHALDTYLPKLVRAGLRVAICEQLEDPKLTK
                     TIVKRGITELVTPGVALNDQILENKENNFLASVFIDKKYFGLALLDISTGEFLLSQGD
                     ADYIDKLLQSFRPKEVLYERQFKDQFVEQFGSKYTIFPLDEWVFTQENANERLLNHFK
                     VKNLKGFGVHEMVHGVIAAGAILNYLNITEHRNIGHISSLSRIDRDQYVWLDKFTVRN
                     LELLMPMSEGGKSLLDILDRTTSPMGGRLLRRWIAMPLVNRQKINDRHDVVECFITDS
                     DLSDKLKSCISSTGDLERLISKVAALRVNPREMVQLRQSLLAATNAKSLCEKSGNAIL
                     KRIADQINPCTDICNRLGRELQEDAPNLIAKGKVIAAGVSAELDELRSITTSGKDYLL
                     SMQEREIENTGISSLRIGFNNVFGYYFEVRNTHKDKVPAGWMRKQTLVSAERYITEEL
                     KEYESKILGAEEKIIGIETRLFNDLVLSMGDYISTIQLNANLIAQLDCLISYATVAIQ
                     NHYCRPSMNDSLIVDIKEGRHPVIEKLLLPGDVYITNDVYLDPEVQQMMMITGPNMSG
                     KSALLRQTALICLMAQAGSYVPVLSASLGIIDKIFTRVGASDNISMGESTFMVEMNEA
                     ASILNNLSDRSLVLLDEIGRGTSTYDGVSIAWAIAEFIHENPRARAKTLFATHYHELN
                     EMEKTFSRIRNYNVSVKESGGRVLFMRKLKPGGSEHSFGIHVAEMAGMPRSVVERAKQ
                     ILVQLEGSRDQTHISKPTEKIAKSREGFQLSFFQLEDPVLKQIRDEIKNLDINGLTPL
                     EALNKLNEIKKLSGL"
     gene            17403..17942
                     /locus_tag="PLK75_00610"
     CDS             17403..17942
                     /locus_tag="PLK75_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOY37775.1"
                     /translation="MKNDTMNVLDIIIIIPLVWFGFKGLKNGLIKEAGSVAALVLGIF
                     GALEFSGFTEKYVHNFTSVSAQYIPLIAFAVTFVAILIVVYLISGLLDKFVNAVKLGW
                     LNKLGGIVFGVLKIAFIISALFYMLHQVNTQKKWISESYLNTSLLYKPIAAIIPTVYP
                     YIPDIEINKHEQTKEDEKK"
     gene            18041..18493
                     /gene="rimP"
                     /locus_tag="PLK75_00615"
     CDS             18041..18493
                     /gene="rimP"
                     /locus_tag="PLK75_00615"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOY37776.1"
                     /translation="MIEKKDIEKLATGFLGETQFLVDVQVTQGNAIRVFVDDLNGLDI
                     DACKLLSRKIESALNRDVEDFELEVSSPGLGKAFKVMQQYVKCTGKMLEVTLKNGIKT
                     EGILREVNAENIILETIRKKTKKDPAPVPEATSILFSEIKTANEKLIF"
     gene            18499..19746
                     /gene="nusA"
                     /locus_tag="PLK75_00620"
     CDS             18499..19746
                     /gene="nusA"
                     /locus_tag="PLK75_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOY37777.1"
                     /translation="MTKKMEHNNLIETFSEFKELKNIDRPTMISVLESVFKATLVKLF
                     GSDENFNIIINVDQGDFEIWRTRVVVEDNEITDPNKEIKLSEAQKVDDDYEVGEDFID
                     EVKLYDFGRRAILALRQNLQAKIAELEKDNIFNKYKDRIGEIISGEVYQIWKKEILIL
                     DDEGNELILPKTEQIPTDFFRKSDTVRAVIANVELRNNTPRITLSRTSELFLERLFEQ
                     EVPEIFDGLITIKKIVRIPGERAKIAVESYDERVDPVGACVGMKGSRIHGIVRELRNE
                     NIDVINYTANTQLLIQRALSPAKISSIKLNNELKKADVYLKPDQVSLAIGKGGLNIKL
                     ASQLVGYTIDVYREMNEDEEDVDLDEFADEIDGWIIDQLKAIGCDTAKSVLELDIAYL
                     EKRTDLEEETIKEVIAILKSEFE"
     gene            19791..22673
                     /gene="infB"
                     /locus_tag="PLK75_00625"
     CDS             19791..22673
                     /gene="infB"
                     /locus_tag="PLK75_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOY37778.1"
                     /translation="MDTENKPQRLAKIAAELNVGISSIVDFLAKKGHKIDSNPNSKVP
                     FELMPLLRKEFSDSVSLKEQSNDISQRTKERKAVLTEVHSEPVKPEHPKHEVEDKELL
                     IKDSSGLEGPKIVGKINLDELNKPVKKPVEPAKTTTAEPEAKKVEPAKISDSETEKKA
                     DDSKTEKKSPKIVGTIDLDSLNQKTRPDKKKKSDKKTETPEPVADEPTEAPVAEEQPI
                     VVETPVAKEDEPNNFIETKYDKLTGPSVIGKINIDDFVKKEEDIRKKRELEKVERKQE
                     REKEREQKKKRKRIRKETVKVDVTKNKEQNKFKPKETGTKKKPQKEIKPEVSDELVQK
                     QIKETLARLTSTKQKSKTSKYRREKRDIVSQRIQAEIEETEAQKNILKVTEFVSVNEL
                     ASMIQLPVTEVIATCMNLGLFVSINQRLDAETMALVADEFGYKVEFVSAEVLSDISDE
                     VDDEEELQPRPPIVTVMGHVDHGKTKLLDYIRHTNVVAGEAGGITQHIGAYSVVLDSG
                     KQITFLDTPGHEAFTAMRARGAQITDIAIIVVAADDDVMPQTVEAINHASAAGVPMVF
                     AINKIDKPNANPEKIKEQLANMNYLIEEWGGKYQSQDISAKQGLNVDQLLEKVLLEAE
                     LLDLKANPNKRAIGTVVESALDKGKGYTSTILVQSGTLHIGDIILAGSYTGRVKAMFN
                     ERGLKIDTAGPSKPVLVLGISGAPQAGDKFNVMETEHEAKAIANKRLQLQREQGLRTQ
                     KYITLDEIGRRLALGNFHEIKLIVKGDVDGSIEALSDSLIKLSNEEVQVSVIHKAVGQ
                     ISESDVMLAAASDAIIIGFQVRPSIGARRLAEKEQIDIRLYSIIYDAINEIKDAMEGM
                     LSPEIKEKILGAAIIKEVFKITKVGTVAGCIVTEGKILRTARIRLIRDGIVVYTGELG
                     SLKRFKDDAKEVVSGLECGLNIENYNDMKVDDVVEAFEEFEIKKKL"
     gene            22703..23686
                     /gene="dusB"
                     /locus_tag="PLK75_00630"
     CDS             22703..23686
                     /gene="dusB"
                     /locus_tag="PLK75_00630"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOY37779.1"
                     /translation="MFIGSLNLGDKPLLLAPMEDVTDPSFRFLCKHFGADMVFTEFIS
                     SDGLLHEGRKSVEKLNISESERPIGIQIYGHLPESMVEAALIAEAANPDTIDINFGCP
                     VKKIANRGAGSGMMRYPELMADITARVVQAVKKPVTVKTRLGWDENSKNIVDIALRLQ
                     DAGIQALTIHGRTRSQMYKGEADWTLIGEVKNHPAIHIPIIGNGDIHDGPSAKAAFDT
                     YDVDGIMIGRATVGHPRIFAEIKHFLEHGTELPPLNLNETVELATLHFTKSLEYKKLP
                     RAIFEMRRHFCTYFKGIPDFKETRMKLVTSTNPDEIFALLQYIRQRFGEGR"
     gene            23883..24737
                     /gene="rhuM"
                     /locus_tag="PLK75_00635"
     CDS             23883..24737
                     /gene="rhuM"
                     /locus_tag="PLK75_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOY37780.1"
                     /translation="MTNEIIIYQYNEQSVRLEVRIEEDTVWLTQAQMAELFQTTPQNI
                     TIHLRNIYQEGELNKEGTCKDYLHVQNEGARKVKRTIQLYNLDVIISAGYRVKSKRGT
                     QFRIWANQVLKEYLLRGYAMNNRMSRIEDRVHSLMKKVDDIDLQIKTSLPPTQGIYFD
                     GQIFDAWVFVSGLVKSANHSLVLIDNYVDESVLNLFLKRNASVSVRIYTAHITKTLQT
                     DLEKHNKQYAPIEILSYTKAHDRFLIIDNQTVYHMGASLKDLGKKLFAFSKMNLNAQE
                     ILKTIQAI"
     gene            24734..26368
                     /locus_tag="PLK75_00640"
     CDS             24734..26368
                     /locus_tag="PLK75_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37781.1"
                     /translation="MMKKAIILLKAAAFSIMLLCIVFQSKAQTWDTIPGLDIHSMALN
                     FTNYNETLIIAGAFRDDLPYTHSNCVIGWDSINLYSFQGITDYGSAAYATMVYKNQLY
                     LGGMFGSPSNDTDIEKFAVWNGSSWEQVGNAIMSPQGRVTHLLEYRDTLYVGGDYGAI
                     NYNPNLWDLVKWDGSDFYSCRPYAATCFGLAVYNDVLYSGIYGAFINNDGVPVEGFTM
                     YGENNWTSVFDRDGVMFPGCVNSLLTDTINNDLYIGCVQVYRYDGEYFHQIAQMNGQV
                     NRHAMQMYHGDLYIGGMFTQVDGIPAKHLAKWDGEQWSEPGGGVSNSIESMYVYKDEL
                     YVGGYFTQVGGYLPAYGVARYYEPEVEDCHWFRPRIQTAGYQDTFYITQSQPYVFVNF
                     VNNNAYAESWEWDFGDGFSGSGSKDIEHGYYQPGEYTVLVEVTQDGCTKTAEKLIVIE
                     DHTGGITSKTGNDEITLFPNPCYDKIWVPYFCPAGETYRVEVCDITGRVLISDQLKAG
                     NNVYPVFTSMLSPGTYLIRIANEKGSVKTKRFVKQQ"
     gene            26370..27986
                     /locus_tag="PLK75_00645"
     CDS             26370..27986
                     /locus_tag="PLK75_00645"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY37782.1"
                     /translation="MKKIIVLFKTTIISLMLLCSLLQGKAQTWDTIIGMALNARTYCF
                     IDFHDTLILAGNYSNGMPLTNSNSVIGWDGINVYSYPGNIQSTPAKALGLYNGRLYIG
                     GSFDDITNPDDISKLAVWNGTEWERVGTAVVGAGTIYYFQVYRDTLYIGGNYGINFNS
                     INDGYLVKWNDTAFYSCSTYGSASFGTHPTGIAVYNDALYAGLYSCFSVGGGQYVCSL
                     GKYNGQTWANVYDKNGNQFQSGVSSLLTDTINNDLYIAGISVFRYDGESFHLIGTAGS
                     QVNGHAMQMYHGDLYIGGMFSQVDGIPANHLAKWDGEQWSEPEGGVSNSIESMYVYKD
                     ELYVGGYFDSVGGYLPAYGVARYYEPAAEDCHWFRPRIQTAGYQDTFYITQSQPYIFI
                     DFVNNNAYAESWEWDFGDGFNGSGSKDIEHGYYQPGEYTVQVEVTQDGCTKIAEKLIV
                     IEDHTGGVANKAGTGAITVYPNPGSGLVVIKTNNGSAIGRIRISSTLGKQMYKAEISN
                     PEISIDISQWNAGTYLVECKEHGVMKLVVE"
     gene            complement(28261..29097)
                     /locus_tag="PLK75_00650"
     CDS             complement(28261..29097)
                     /locus_tag="PLK75_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="HOY37783.1"
                     /translation="MVAFCMMLLFNQMVFCQLITELPYNKTCFLTTHNSFNCSRNGFL
                     LPNQKYGIQQQLNDGVRALMLDVYEVKGRLYVYHSIQMLGKEPFAEVLKEVEIFLEVN
                     RNAVITLLLENYAPAYLIQSDLHKTGLSKFQYYADSIREWPTIQELIDDNTRLLVFND
                     VNDTMNRQARIFTMWDFFTETHFSVNRMCDFKCSTNRGKSDNPLFVLNHFINGGLGIG
                     SRTQSKKANGMNVILHRCFDCKRLSDKYPNFIVVDFYHLGNPLQAVDSINLLKVQELK
                     SR"
     gene            complement(29286..30518)
                     /locus_tag="PLK75_00655"
     CDS             complement(29286..30518)
                     /locus_tag="PLK75_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOY37784.1"
                     /translation="MSQSFENINIERDIVFAIEDGFDFMGFGIDNPEPLRMDVYFPSG
                     DNMKARPLVICLFGGAFLAGSKERPDMIAWCDSLAHYGYVAAAINYRLGFNPLTSGKG
                     FGADEGVIRALYRSTQDCRAAVRYFINQHERYHIDTTCIFLLGNSAGAITAINTAFMT
                     GDERSADTYETGYGANNHDLGCLDCTGGFRKNKCAVAGVVSLWGAALNPEIIDKDEEI
                     PVLFIHGTADEVVPYDKDYALNFTLGKDLNVFLYGSKSLKEHCDKLGWTHTVLDPYLG
                     QPHCFYACGNIIISELNYDTFPCEYWWPVFYQTIRFLGENNKYCDKKYLPSLENTIEF
                     TINDLSEKGSFELIFKGNLLMNNRLTLYNAIGDVVLTQVIQSDKTQFSLAGKPAGIYI
                     VEIKNHRYSMTQKFTLKK"
     gene            30767..31552
                     /locus_tag="PLK75_00660"
     CDS             30767..31552
                     /locus_tag="PLK75_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY37785.1"
                     /translation="MYKLFRNITITLIFVAGVINFASAQLIRMNNRMVIAGIVVDAET
                     EASMPYVNVYVKHTRTGTITDTAGYFVLFVNIGDTIIFSSIGYEKLHVLAIDTLGDIS
                     RPAWVRLKRKVYELQTVDIIALRRYEQFKYEFTTMVLPENDYTHAARNFPVRPPELDY
                     YTRQGASGFGLIFSPISALYDAFSKEGKERRKLEEIEEQDALKKEVAKKYNPAMVMRI
                     TGLNEPDAIAFMNWCQLPDRLILNATEYQIIEIIMMQFDKYNK"
     gene            31645..32691
                     /locus_tag="PLK75_00665"
     CDS             31645..32691
                     /locus_tag="PLK75_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY37786.1"
                     /translation="MPVLFKPQTTKQVIILLLINMLTISFSIPGMSQSSDTSPVVYTH
                     TVRQSMHARMVKGIARLFLPKHNLEKKLKQKNFVSEPAQIPRTVLRNFIIDEYDICGR
                     KVYCISPKNESCGKSILFLHGGAYINNIFRQHFQFAGKIATLTGCRVIIPDYPLAPSA
                     SYSDAYIMLTAVYASLLRETANESIIFMGDSAGGGLALAFAQYLSQNKTPLPSQLILL
                     CPWLDVSMTNPEIQSILKKDPILRTPGLVIAGKVWAGSCDTKNPMISPIYGDLANLPH
                     ISMFIGTYDILYADCNKLSTKLQSCNVHTRMFVFPKMFHDWMLYPNLREADAAINIIQ
                     GLITQGPRYDTESK"
     gene            32977..33432
                     /gene="rplM"
                     /locus_tag="PLK75_00670"
     CDS             32977..33432
                     /gene="rplM"
                     /locus_tag="PLK75_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOY37787.1"
                     /translation="MNTLSFKTLSANRSTVQKEWLLVDAEGQTLGRLASGVAYLLRGK
                     HKPDFTPHVDCGANVVVINAEKIVFTGKKLTDKEYISHSGYPGGQKIQTADELMAKYP
                     ERIIENAVKGMLPKNKLANQLFRNLHVCVGSEHPHAAQKPKKIDINEIK"
     gene            33438..33824
                     /gene="rpsI"
                     /locus_tag="PLK75_00675"
     CDS             33438..33824
                     /gene="rpsI"
                     /locus_tag="PLK75_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOY37788.1"
                     /translation="MDTVNAIGRRKAAIARIYLSDGKGNITINDRKIEEYFPLLQYQY
                     LAKQALLVTGLLEKFDIKANLEGGGIKGQAEAVRLGIARAIIKADAEQRPILKSNGFL
                     TRDARVVERKKPGRKKARKRFQFSKR"
     gene            33934..34812
                     /gene="rpsB"
                     /locus_tag="PLK75_00680"
     CDS             33934..34812
                     /gene="rpsB"
                     /locus_tag="PLK75_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOY37789.1"
                     /translation="MPRISFDELLNAGVHFGHLRRKWNPHMAPYIFMERNGIHIIDLY
                     KTSAKIDEAANALKQIAKSGRKILFVATKKQAKDIVAEEVKNIGMPYVTERWPGGMLT
                     NFPTIRRAVKKMTSIDKMITDGTFDTLSKREKLQVTRQRAKLEKNLGSIADLSRLPSA
                     LFVVDVMKENISVREAQRLSIPVFAIVDTNSDPNGIDFPIPANDDATKSIQLIVGTMC
                     NAIKEGLEERNQEKDKLMDRKADDEIEEDEDDEIIENKSTGNLIVEEEEEVAKTAPKK
                     TLGRKKISKPIIKTEE"
     gene            34834..35661
                     /gene="tsf"
                     /locus_tag="PLK75_00685"
     CDS             34834..35661
                     /gene="tsf"
                     /locus_tag="PLK75_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOY37790.1"
                     /translation="MALITAADVSKLRKITGAGMMDCKKALTEADGNFERAQELIRER
                     GLAIANKRADREASEGAVLSCSANDKKAGVVIALNCETDFVAKNQDFIALANDILKLA
                     VEKLPADLDALKSTKLGNKTVEEIVLERSGITGEKMELAYYDFVKANYVAYYIHMGNK
                     LATIAGFNQPVDEQVGKDVAMQIAAMDPVAVDKDDVPQHIIEKELEIGREQARNDGKP
                     ENMLDKIALGKLNKFFKDSTLLNQEFVKDSKKTIAQYLHEQDKNLTVTAFKRFGLKN"
     gene            35735..36451
                     /gene="pyrH"
                     /locus_tag="PLK75_00690"
     CDS             35735..36451
                     /gene="pyrH"
                     /locus_tag="PLK75_00690"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOY37791.1"
                     /translation="MSAPEYKRVLLKLSGESLQGSQTSGIDEKKLMEYAVQIREIQTL
                     GIEVGIVIGGGNIFRGITGTQKGFDRVTGDQMGMLATVINSLSLHNALTSMGAKVKLF
                     TALTIESVGDRFSKPKAVEAMKNGHICIFAGGTGNPFFSTDTASALRAVETDCDVLLK
                     GTRVDGVYTADPEKDPTAVKYDTLTFGEAYEKGLKIMDLTAFTLCRENKLKVIVFDMD
                     TPGNLKKLLSGNKIGTLVSI"
     gene            36470..37036
                     /gene="frr"
                     /locus_tag="PLK75_00695"
     CDS             36470..37036
                     /gene="frr"
                     /locus_tag="PLK75_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOY37792.1"
                     /translation="MNTDDVQFYLESAKEEMNGAIEHLNREFYKIRAGKASPAMLESV
                     KVDYYGTISPLSQVANLSSPDAKTIFIQPWDRGVIEAIEKAIQAANLGFNPSNNGEVI
                     RIIVPPLTEERRKQLVKQVKNEGEAAKVTIRNHRRDFIEEFKKLKKDGLAEDLAKDAE
                     AKIHKMHEDYIRKIDEIMLRKEKEIMTV"
     gene            37116..38726
                     /locus_tag="PLK75_00700"
     CDS             37116..38726
                     /locus_tag="PLK75_00700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOY37793.1"
                     /translation="MKKSVLLFIALVVSLPIFSQDKSDWVAGYPDGCTSITAGKMATT
                     DGSVFTSHTDDSHRTRSEIIIVLGQDYSTGSKMPLYKRIACDTTAMPSYQNIRIGEIP
                     QAKHTYHYINTAYPCMNEFQLGIGESTFGGRDCLQSDAGLIDCQRLCQLILERCKTAR
                     EAIILTDALTKEYGWNDYGECLTISDKNEVWHLEIVGPGKGEVGAIWAAQRVPDDHIS
                     CNCNASRILEIDLNNKEYFMASDNIFTMARDSAWWNPEKEVFRFAYAYAPESRKSLAS
                     RRREWRIFDLLAPSLHLDPNDENYPFSVKPDSLVSIDKLIVVFQDYYEGTPYDMTRCI
                     TTTDKDGKTTISPLANPFMPYDELSLHNVSGGWYSTNHDGSIQFLGERTIARWYTMYG
                     TLIQSRSWLPDEIGGVAWIALDNIATSVYVPLYCSITDLPQSYKTPGRTKGYTLQSAW
                     WVFNRLGTLAAQRWGDMRHDVDAAWKPWQKELIRNQNNIDKFAQNMKSTKKRVEFLTQ
                     YSNDQANKAVTKAIELGDFLWTKYDEKF"
     gene            complement(38738..39322)
                     /locus_tag="PLK75_00705"
     CDS             complement(38738..39322)
                     /locus_tag="PLK75_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY37794.1"
                     /translation="MMVKKTHLIALMFVAAIIFAGCNKTPHASFVTDKAVYNAGETVV
                     LTNTSVDSHHYVWTFPDNQISTETNATYVIPNTSSGLLYFKLEAFSRKGDLTDEIIKT
                     VNVLPKLGSAMFWKSEHYDDITVTFYGPTQHISENFDLAPECGNANCANFYDIPIGTY
                     AYEATDGVFNWTGELEILENACSKKKIIYNEADE"
     gene            complement(39451..40521)
                     /locus_tag="PLK75_00710"
     CDS             complement(39451..40521)
                     /locus_tag="PLK75_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOY37795.1"
                     /translation="MKTFEPTDNPLLIAGPCSAETEKQLLETAQALASIPDVKVFRAG
                     VWKPRSRPNSFQGRGAEALPWLKMVQDNFNLPVAVEVAEPEHIEMLYKYGIKIVWFGA
                     RTVSNPFSVQKLADALKGSNFSVMVKNPVYPDIELWSGTVERLLDNQLEDIAAIHRGF
                     FPFEKSELRNTPMWEIPIEFRRRFPEIPMICDPSHIAGNKKYISDIAQRAFDLNFAGL
                     MVEVHISPKAALSDSRQQLSPAEFFALIGSLTARNVQSEDADFKNHLEDLREKIDVID
                     FQLLELLSHRMKYVDEIGEYKKKNNVAVLQLKRWGRILESRLEQSNKAGLSKEFIKQL
                     LELIHKESIARQTEIMKRTNRQ"
     gene            40686..41705
                     /gene="fbp"
                     /locus_tag="PLK75_00715"
     CDS             40686..41705
                     /gene="fbp"
                     /locus_tag="PLK75_00715"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOY37796.1"
                     /translation="MAAFRVLTLNEVIIQKQSAFPYAKGELSRLLSHIGLAAKIVNKQ
                     VNKAGLVNILGEANKINVQGESQQKLDVFADDVFKKAMQASGIVCGIASEENDEIIIF
                     DDDLSLEGNYVVCIDPLDGSSNIDVNVSIGSIFSIYRRLSPVGSKAELRDFLQPGIKQ
                     IAAGYVIYGSSTMLVYTTGLGTTGFTYDASIGEFCLSTTYMVTPKSGKIYSINEGNYV
                     KFPQGVKQYIKYCQEDDKETGRPYTSRYIGSLVADFHRNLIKGGIYIYPTTASSPNGK
                     LRLLYECNPIAFLAEQAGGAATDGRGKRIMEIIPTSIHQRTPFFTGSKNMVEKAEQLM
                     REFGD"
     gene            41710..42804
                     /locus_tag="PLK75_00720"
     CDS             41710..42804
                     /locus_tag="PLK75_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37797.1"
                     /translation="MTFAAFIRFKLVFVFISCIYISIAVAQSPGVKKLHLLDAVLTET
                     SGLIYTNNMLLSHTDSGGEPCLYEINIHDGHILNTYCYNTIHNYDWEDIATDGNAIYI
                     GDFGNNSGSRNPLKIYRIALDDIGNPEATIDSISFRFADQEDYTPMFRKTDYDCEALF
                     AAGGRLYVFTKNWNNKKTKMYSIPANPGDYSAEPILELDVNGLITGAAFNSNTQVLAL
                     IAYEKTFPFNPLMFLIRFSGNFDHIINYSRITLQINNMQTEGITWADDHTLLITNESF
                     LKTKSHLMKSDLNTCTLLPFIKDSLPDPEKLLETGYNFNLTISDKNGNKVFQGKLRKF
                     YRNQDQWLGVKKPARIEWHAQKASGWFMWQ"
     gene            42847..46215
                     /gene="mfd"
                     /locus_tag="PLK75_00725"
     CDS             42847..46215
                     /gene="mfd"
                     /locus_tag="PLK75_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOY37798.1"
                     /translation="MSAFPENIYTNDTRIQQIAGITETGQGNHISLCGLAGSASAYCL
                     AALFKKTGSRICIILDDKEKAAYFYDDMVLLSGKDNVLFYPSSYKRSLEYEKTDSTNI
                     VLKTEALERLSNSSKPYIVVSYPAAISEKVLKKSDLKKNTMHIGSNDQLSMDFVIELL
                     FEFGFTKEDFVYEPGCFAVRGSIIDVFSYGSEFAFRIDFFGDTVESIRAFDTGTQLST
                     QKLRSINIVPNIHDFGQEQERISFFEYLGTKNLICFENKNAFLNQIEKVFDAAILKSK
                     TDKGLSDELAYPERFLVAHNEMTRLLTEFNTIEFRNVRDESVSLSVEFNTSAQPVFGK
                     EFDLLGEDIIRRKEAGYTVNILSENPKQHQRLREIFEVRWGDIGFETPQFTVHEGFID
                     HDHKVCIYTDHQIFERYHRFKVKDSFSRKDAISYKELQSINPGDFVVHIDSGIGRFGG
                     LTTIENNGKLQEVVTIHYADNDVLFVNIHSLHRISKYRSKDDTSPSIHRLGSGHWKRL
                     KEKTKQQLKDIARELIVLYAKRSEQHGFAFSADNYLQNELESSFIYEDTPDQIKVTRE
                     VKNDMEKTMPMDRLVCGDVGFGKTEIAIRAAFKAVCDGKQVALLVPTTILALQHYQTF
                     RERLEKFPVTTSYISRLRSPSEIKSSLKDLKEGKLDILIGTHRLVASDVEFKDLGLLI
                     IDEEQKFGVAVKEKLKKLKLNVDTLTLTATPIPRTLQFSLMGARDLSVLSTPPPNRQP
                     IVTELHTFNHSIIKDAIQYEVNRNGQVFFVNNRIDNIYNIQATIKKLCPEVSAIVAHG
                     QMEGKQLEKIILDFMRGDFDVLISTSIIENGVDIPNANTIIINNANNFGLSDLHQMRG
                     RVGRSNRKAFCYMLAPPEELLTPEARRRLKAIEEYNELGSGFNIALQDLDIRGAGDIF
                     GAEQSGFIANIGFETYNRIISEALTELKDTEFNDQFGNQDPEKTGIADLHEYEVADCS
                     IETDFEVLLPSDYISSTSERINLYRQLDEIKTDEELEVFRNNLIDRFGKLPQQAIELL
                     DVVTLRRMAMQLGFIKIIIKTNLMIAYFSFDQQSAYYSQKTFASILQYIQNHHKKMFL
                     KEHNGKLSLRFEGISNIQEAVQRIQPLADWVNQA"
     gene            complement(46224..47048)
                     /locus_tag="PLK75_00730"
     CDS             complement(46224..47048)
                     /locus_tag="PLK75_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOY37799.1"
                     /translation="MKKFTSFLVLAGFSIIIACNKPVQPVQENKQESKNTGEHLHMAL
                     LWYQRSAEMTACSYQAYNVAKSALLNNIKNDTSKLPKAVILDIDETLLDNSPFLAERS
                     VRGEDFSPEAWAEWSEMEKATALPGAVDFIKFALDNGVEVFYVSNRLTGELDWTLKNM
                     ASLGFPEVNKANFLLKSTTSDKKMRRDSISEKYSVVLFVGDNLCDFSNEFDDRSKNFG
                     KDAVENNIDLFGTKYIIIPNPTYGTWEKPLYDKSETPKPEQRKKMLISYEDLKNDK"
     gene            47145..50603
                     /locus_tag="PLK75_00735"
     CDS             47145..50603
                     /locus_tag="PLK75_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOY37800.1"
                     /translation="MKNPRRLFLVFLFLYIGFNAYPNPGDTTLVQCFSFDSIYARRGI
                     FLFPPQGTSYEKVLMYYTIKCDPLTPWDSYNCGEWDYTTFTHIYEHTGDYDSTLYFHP
                     YFTVNGMQPYSFSYLNSPLAQPFPRREYFWQHTNTISMNLADIGSGSIPVETPFGNAE
                     NDAKHLVLFKAAELTEGGLNAGNITGLRFYAMPTGADVQRLRVRIKPVAIDDIGGFNY
                     LFTGFSTVFDRNVSIDEEGWFSIDFLNYFAWDGVSNILVEFSYSGISGSLPLAGEELL
                     YNCCLTSEAPDNAIRFNGPDYIEVPATSVADLNQKISIAFWLNGDHAIQPQNDMLLEA
                     CDASKNRKLCIHLPWGDGSVYWDAGNNGAGYDRINKSASPDEYKGKWNFWVFTKDAST
                     GVMQVYLNGVLWLSGTGKTALLNNISTFVLGSNVDKNPEYCYDGMLDEFMVFNTALNQ
                     TDIENIMYHPLEETSALWNNLILYYPFDEGSGSLLTDASQYQHNTNMMGMPDWISYHG
                     INRYKYFTAGMLRPNTIFEQAEYESTLDSLTVFDTLYSPQVQVITYANQDNYTIVATD
                     TSLYYPARFLTAYDVDGVALDSVYFSDATIYNASLEYYSQPFEVINTYQVQNFVTPYG
                     IGLNLGSTGFTWVYDVTDYISLLHDSVDISAHNTQELLDLKFALIEGTPSRNVIEFSQ
                     LWRGNYSHHGIASDEQLPGVKILANPNASMFTIRTRTTGHGMEGDGNCSEFCPTYHKL
                     YVDGAQRYEWLNWKECSTNPIYPQGGTWIFDRAGWCPGNFADTYDWDITDWVTPGDSV
                     LIDYGMEQYPGSNGEGNYQIAVQAFQYSAPNFLNDAAIDVIVAPTDADEFVRYNPVCS
                     NPVIRIKNNGATTLNSLEINYGISGNTTHQFIWTGNLDFLETEEVILPALSWLDFTGD
                     SQSFEVTVTNPNNTEDEYEPNNHSVSHFVLADVYDQVIMLVVKTNNYGSQTYYDVTDS
                     EGNILVSRDNLDNNTYYYDTLDFDPGCYVIHIYDRDEGFIGQDGLNFWYWANTPYDDG
                     TGFAKLRVPGGAYVKHFNADFGSFLTYQFIYPDASYILNEKAKKDYFSLYPNPCSELV
                     NVEFYEVPGTSDYISIFDVSGRQVKQIHHMSNDICIDVSDLQKGMYVLKYISGNITST
                     RSLIVQ"
     gene            complement(50612..51829)
                     /locus_tag="PLK75_00740"
     CDS             complement(50612..51829)
                     /locus_tag="PLK75_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOY37801.1"
                     /translation="MSSQNQPHQILTHYNLMPQEEVLEVQNTERRFTIGIPCDDSGLE
                     WRVPLAPLAVEQLSAHGFDIIIENESGRNANFSDTSYADHGALITNEKEAIFKCDIVL
                     KVGPFTVEECKMLKGNQMIVTSLHASTQPDEYFHILMQKKLTAIAFEMIQDRYARFPI
                     VRSMSEIAGRSSILIASEYLSNVHKGKGEMLGGITGVSPSEVVILGAGTAGTYAASTA
                     LGLGAHVKVFDHSVYRLDRLQQQLGIQVFTSTIQPSVLENALKTADVAIGAMRITNDT
                     NMMLVTEEMVMKMKKNSVIIDISIDQGGCFETSKLTNHKTPVFKKHGVLHYCVPNIPS
                     RVARTATYALSNILAPKLLGFHTSGSINRFLKSYPGMRNGVYIFNGILTNESIGKKIG
                     LFSRDIDLLLAAL"
     gene            complement(51955..54084)
                     /gene="rnr"
                     /locus_tag="PLK75_00745"
     CDS             complement(51955..54084)
                     /gene="rnr"
                     /locus_tag="PLK75_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOY37802.1"
                     /translation="MSSKFNKNNIPNQHSAKILLQQVLNVFAGSPQKLFNYKQMAARL
                     KVRDLLEKEEVKKVLTELLKSKNITEVYEGKYKYIMPKTYVTGKVDVSRRGYAYVLSE
                     EAPEPVFISPRNLHNALHGDTVKIQMYASRKSKQVEGEVIQIIERARTQFAGIIERNK
                     KYAFFMPDDLRIPYDIYIPLGNLNGAKSGEKVIVSITEWHPESKNPTGTVVKVLGKPG
                     DNEAEINAIMVEFGLPYEFSNEMIREAEKIDGSIPVSEIKKRRDFRETLTFTIDPEDA
                     KDFDDAISYKVLDNGSVEIGVHIADVTHYVNPATVIDTEAQERATSVYLVDRVVPMLP
                     ERLSNEICSLQPHVDKLCFSAVFILDSTAKILDEWYGKTVINSNRRFNYEEVQKIIDT
                     GQGDLSKEIAAVNSLAHLLREQRFRKGAFNFESTEVKFNLDEKGKPLGVYFKEPKESN
                     WLIEEFMLLANRKVAELLSKTGKVSVYRIHDEPNPEKMESFSGFIKKFGYSIETKNAV
                     NLGKSINGLIEKVQGKPEQNVIQSLAIRSMAKAEYSTENIGHYGLAFTHYTHFTSPIR
                     RYPDMMVHRLLENHLTQGRTGVQDNYKWLCKHSSDMEQRAVQAERASVKYKQAEYLSD
                     KIGLPFEGVISGVTDWGFFVELVENKCEGLVHIRTLIDDFYFFDEDNFCIIGKTTGRK
                     FTLGDTVKVAITGVNLSKKQIDMELLR"
     gene            54181..54768
                     /locus_tag="PLK75_00750"
     CDS             54181..54768
                     /locus_tag="PLK75_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37803.1"
                     /translation="MKTTTMIKTLAVVAIIGSTVAFSGCKGKKEASNPGKGAKEVVIP
                     CKNEGRSDKEYFRADNSSTSSDMSLSREKALTLAKQRLASLINTKIKSVTDRYVNETE
                     VGKNSDFEQKFENLTREVVNQTLSDVAIVCEKTFQEADGKYTTYIAIEVNKDALLNGV
                     SNKVSKDDKLRVDYDKMKYEKIFNEEMEKLEQENQ"
     gene            54902..55840
                     /locus_tag="PLK75_00755"
     CDS             54902..55840
                     /locus_tag="PLK75_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOY37804.1"
                     /translation="MQIILSQISMLGLLAVIGAVALKTRLLQEETMNGIVKLITKLTL
                     PLLIFSTFAGNNINGEILKNSSVIIIVSMLSIVMFYLFAGMSARILHLNVENTALHKA
                     SAMFGNVVFLGFPVINAIFPGGEGLIYATMYQLGQNTLIWTWGVYILNKGADKTSKEN
                     WKHLINPVTIAFAAGLLFMAMPFRIPEIILKPISDIGHTTSYISMIYVGAVLVKANPL
                     RVFVNLRSYLVSVNKLVLTPLIILLLFKYAGLSQLFHVSTTALGVMVMLSGMPCMILI
                     SVLAKDLGLNDRQSVENIFLSTILSFGTLSLLFWLL"
     gene            complement(55837..56640)
                     /locus_tag="PLK75_00760"
     CDS             complement(55837..56640)
                     /locus_tag="PLK75_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOY37805.1"
                     /translation="MPRRKIRRNKKKNNFLVGVFIFLLLLIAAATVYLVFFSGDNTRV
                     KKERYTGDTSFQSYVMHFKDYTVFGIDVSEYQASIDWKKVASEKSMYFVFIRATAGNS
                     KTDRNFITNWYAAGEQKLIRGAYHYYRPNENSTGQAQRFIETVTLQKGDLPPVLDIEQ
                     YSHVQNKNSLKTGLLNWLEIVEKHYQQTPIIYTSPNIYRSMFNGDKRFEKYPVWISSL
                     NVSKEPSHIAAQWKFWQFSHEGKVAGIKGKVDIDLFVGTYNDLVNFTIH"
     gene            complement(56650..57627)
                     /locus_tag="PLK75_00765"
     CDS             complement(56650..57627)
                     /locus_tag="PLK75_00765"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOY37806.1"
                     /translation="MKIAVVGATGLVGQKMLKVLAEHNFPVTTLIPVASERSAGKNIL
                     FGGKEYPVCCLQEGLAMQPHIALFSAGKDVSLEWAPRFVEKGCTVIDNSSAWRMHDDI
                     RLVVPEVNATLLDKKPQIIANPNCSTIQLVAVLYPLHKLYGLKRVIVSTYQSVSGSGY
                     KGVKQLLGERAGSTLHDAYPHQIDLNCIPHGGNFLANSYTDEEMKLVNESRKILSLPQ
                     LALTGTVVRVPVIGGHSESVNIEFQEKPDINQIRRTLSETQGIVVKDIPTANIYPMPI
                     WAEDRDEVFVGRIRKDESHEYAINLWIVADNLRKGAATNAVQIAEYLIK"
     gene            complement(57745..60675)
                     /locus_tag="PLK75_00770"
     CDS             complement(57745..60675)
                     /locus_tag="PLK75_00770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY37807.1"
                     /translation="MKKLWIIALIPLVLFTSCNNKGYKTKTAKDANGYTYEYVTNDPV
                     NARIYTLGNGLKVYMAVNKDEPRIQTAIAVKAGAKNDPRATTGLAHYFEHMMFKGTDE
                     IGTLDWGKESALIQQISDLFEKHAAETNPEKKLAIYRQIDSLSQEASKYAIANEYDKI
                     CTMMGATGTNAWTSYEETVYVNNIPSNESERWVKMESERFRDLVLRLFHTELETVFEE
                     FNMSQDNDYRKSYYKVMSELFKKHPYGVPVIGIGEHLKNPSMVNIMKFKADYYVPNNI
                     AICLSGDFDPEEMIKTIDTYWGDWKPNENVPQFTSPKEDPITAVKEFSVTGPDQESMS
                     ISFRTEGLHNDQDLFLTLIAEILSNGEAGLIDLNLVQKQKVLRAGAYVNNMNDYGVFN
                     LSASPRQGQKLEELKTLLLGEIENIKKGNFDEWLLEAIINQRKLSQIQAYESNYAVYE
                     FVDQFISGRSREDILKYNDRLAKVTKQEIVDFAKTFFADNYVVVYKRNGANDNTYHVE
                     KPPITALNINRTTESEYVTDLKKMNTKDIEPSFVDYKNLIKTQKVGTAIDFSYIKNET
                     NELFDLYYIVDIGKENDAWLPVAINYLEYLGTSDKTAEDLKKEWYKLGLSFGVSTGDD
                     QSYVYLSGLDENLEKGIELLEKILAGAKVDKEAYANYVDDIIKKRNDAKLNKNTILQS
                     RMMNFSKYGQLNPTTDLIPEADLRAMDPQQLVDLIHQMLGYKHKIFYYGPREQSKMAE
                     LIQKYHKTDATLKETPPRKVYPEKDYTAPTVYFVNYDMVQTLIALVSKDTKYDPLQIP
                     IIRMFNEYYGGSMSSIVFQEIRESKGLAYSSYAGYRTPSEKGRSNYVFGYLSTQPDKM
                     TEALDALTGLLNELAMSEQLFGTSKDAIIKQINSERIIKSDIYWNWQALQKLGIDHDY
                     RKEMYADIQKFTLNDVKSFFDGHIKDKKYDILVVGPKDKINFTLLKKYGKVEELTLEQ
                     LFGY"
     gene            60814..62871
                     /locus_tag="PLK75_00775"
     CDS             60814..62871
                     /locus_tag="PLK75_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOY37808.1"
                     /translation="MKTSNIITIMAIIPLCILSCHQPEKKTATKEVEPFNYVAEQFAD
                     IRILRYQVPGFEKLGIKEKKFLYCLQEAAYWGREIFYDQNYKHNLCIKRTLEAIVENY
                     QGDKNSATWVRFMEYTKRFWFSNGIHHHYSNMKFLPGFSEDFFREQVLSVKPELLPLS
                     EGETPEMLVEKLIPILFSPSVAPVRSESDTRKDLIASSATNYYEGLSQQEVERYYKTR
                     TNKKDSTPISYGLNSKMIKKNGAITEQVYKHEGMYGAAIEKIMYWLTEAKKYCDNAEQ
                     TVVIDKLIEFYRTGSLETFDEYSILWVKNSNVKVDFTNGFIEVYADPLGYRGAWQSVV
                     YVLDEDLTRKFANISAKAQWFEDNSPIMPEHKRSEVVGVSYKVINAVAEAGDCSPSTP
                     IGVNLPNSNWIRSQYGSKSVSLGNIETAYDEASKTSGVLDEFYLPDQKKRIEDFGALS
                     GKIHTALHEVIGHGSGKIMPGVGTPKETLKSYSSALEETRADLVALYYIMDPVVIDLG
                     LMDTIATAYAEYDNYIINGMLKQLVRIEPGQQIVQAHMRCRSLISHWCYEKGKGENVI
                     EKVYENGKTYYKINNYARLRELFGELLREVQRIKSEGDYEAGMKLVETYGAPVDRKLH
                     KEVLDRYAKLNVAPYNGFIQPQMEAVYKNGEWVDVNISYPDSFEKQMMFYAKEYKILP
                     TYN"
BASE COUNT        19261 a        13017 c        13475 g        17247 t
ORIGIN      
        1 cgaacggaat ggagtttctt gaaatcctcc gaaaaactcc agccgatatt gttctcatgg
       61 atatcgaaat gccggtgatg aacgggatcg aagccaccga gaaagccatc agtcagtttc
      121 ctgggttgaa aatcattgcc cttacaatgt ttaccgacga cgactacatt caaagtatga
      181 tggatgcagg agtaaaaggc tttctcatta aaaatgtaaa caaagaaact ctcgacaggg
      241 caataaagac ggtgtatatg ggcggaaatt attattcaga agaattgttc aactttttta
      301 ccaaacaaat cagcaaagaa gaacctgtat cgaaaaccga ttttaaactt acccgccgcg
      361 aaaaagagat actgcaactg cttgcggaag gcctaagcaa caaacaaatt gccgataaac
      421 tgttcgtaag cgaacgcacc attgtggggc acaaatcgaa cctgctgctg aaaactgggt
      481 gtaaatctac tataagtctt ctctcctacg caattaaaaa caaactagtg gtgatataat
      541 taatcaaaat aatttttcat ttatttctca gatttatagt aagtatctta acctcatttc
      601 aagagattta aaaacgatga ttttctgatt gaaatcacaa taatttaaac ccaaatttgt
      661 taatacgata gcgttaaata aaataaatca atttgacact gaacggttaa acccatcttt
      721 tttaatgaaa atactagtac tacttatggt tgttctgtgc gaatgtatct catcagcaat
      781 agcacaaaca aatttttacg accaacttgc tgattcggct tatgttttaa caaagcagaa
      841 tgttagatac gatccatcct atgttgtgat tgcctaccca aacggagatg ttcctgcgaa
      901 taaaggtgta tgtaccgatg ttattgttcg tgcctaccgc aaattgggga tagacctaca
      961 gaaagaggtt catgtcgata tggtggcaaa ttttgatcag taccccaaaa tttggggact
     1021 tagtaaacca gacccgaaca tcgatcacag aagagtgcaa aacctcatga catttttttc
     1081 gagaaaagga acggttctta aaaacagcat agttcctgaa gactattctc ccggtgatat
     1141 tgtttgctgg aatttggatg gtaaagccac acacattggt atagtatcta aaaggagatc
     1201 aatcgatggg aaacggaatt taattattca taacatcggc gaagggcaaa tattggaaga
     1261 ttgcttgttt aaatggaaaa ttataggaca ttaccgctat aaaaagtaac agaattcatt
     1321 aaatcatttt acgtcagata aattctgtgc atgaaaaact gatacgctgt aagaaaaaat
     1381 ataagcctca aaatcaaaat gtattaaata ccaaatgagg ctacatccat tgattaatga
     1441 tttcattaat aattaatcgg ataattgggg aataaatcgt gcacccgtcc taaaaaggaa
     1501 cctacttaat acatctacaa ccattactat caaaggaatt taactgtttt cagcaagcta
     1561 ttattattta ttaatacaat tatttcgcgc taaatacttc ctaaaagtac tggtttttac
     1621 tcaaaaccct tctcattatt acatccgaaa atcagtaata agctacttta aaatttgcat
     1681 atcgcgctaa tacttttacc atagaaattg aagcgaaaaa aacggaaaca ataagttagg
     1741 taaaccaata aaaaattaaa gtcatgaaaa caacaaaaaa agttttaatc gtagacgatg
     1801 acatcgacgt aatcacagtg ctggaaacca tcttaaaaaa agaaggttac acagtaattt
     1861 cggcaaacga caaggtagaa ggtaaaaaac tggctctggc cgaaagaccc gaccttgcaa
     1921 ttcttgacgt gatgatgacc acacattacg aaggttttga acttgccaaa gatcttatcg
     1981 aagatccaaa acttgccgga atgaaagtac tgatgcaatc atcgatagat ctgattacaa
     2041 ccaccaattc gagtgttcag gcaatggcac gcgaataccg caaagacccc agctacaggg
     2101 aactgcaggt gctgctgata aaagacatct tgtcgaacaa cgcaggcgtt gactacaggg
     2161 ctgaaaacgg cgaatcgatt tggcttccgg ttgacggatt cctgagaaaa ccggttgatg
     2221 cagccagatt gctgcctgaa atcaacaggg ttttatcgta gaagaacttc catccggggc
     2281 agacacaact tggtaagcag caactgctgc ttatcatggc cccatataag cagagattca
     2341 ttcaaagatt aaaaccagat agaaatgaaa aaaaatgttg aagcaatatt gaacgaacac
     2401 cccaatgcag gccgcgatag tttaattccc atcctacagg aaatacagaa cgaagaaggc
     2461 tatatttcgg aaaatgcagt tattaaagtt ggagaatatt tgcaaatacc ttccagtaaa
     2521 atatttggtg ttgcaacatt ctacaatcaa ttccggttcc gtcctttagg taaattccac
     2581 attcaggttt gccggggaac agcttgccat gtgctggggt cgaaaacggt tctcgaagaa
     2641 cttgaaaaga aattaaaaat aaaagccgga caaaccacaa gcgatggcaa attcagcatc
     2701 gaagttgttg catgtatagg agcttgcgga ttggcaccgg taatctcaat aaacggtgaa
     2761 ttttatgcca aggtaaccac gaaggcaatt aacgacatta tcgattcata taaaaatcag
     2821 gaggcttcaa tatgacggca acaaaatttg tgaacaaaga tttccttacc aaggaagtgc
     2881 ttaaaaagtt cgataaaggt cagccctcag cagaaactgc aaaacggctt gccacaatac
     2941 gaaacgaaaa gaatcagaaa ccggtgatct tcattggggc gggaacatgt gggctaggtg
     3001 ccggagcagg taaaaccata gtagcaacaa aaaaatatct gcttaacaca ggaatcgacg
     3061 ccgatattat agaaacaggt tgcatcgggt tgtgtgctgc agaacccctg cttgatgtgc
     3121 agcttccggg attcaataga atttcgttcc gaaatgttac tgaagataaa gtagaatcaa
     3181 tactgaattc ggttttttca aaaaaactcc cttcagaacc cgccctggtt caattcagat
     3241 cggagggctc caatgtttgg gacaatgtct cgttcatgga agaccatcca ttcttcggtc
     3301 cacaaataag actggttctg aaaaactgcg gtataatcaa tcctgaaagt attgaagaat
     3361 atatagcacg tggtggttat accggttttc tgaatattat tcatcattac aaacccgttg
     3421 aggtctgcga tttaatagaa aaaagcggtt tgcggggccg tggcggaggc gggttcccaa
     3481 caggtaaaaa atggagattc gcttttaata ccgaagccga tcaaaagtac ttaatctgca
     3541 atgctgacga aggcgatccg ggtgcgttta tggaccgcgc ggtaatcgag ggcgatccac
     3601 atcgtttgat tgaaggtatg gcagtagcag cttatgctat cggagcaacg aaagcatacg
     3661 tttatatccg tgcagaatat cctctggcaa tcaagaggct gcaaattgct cttcaggatg
     3721 ctagggcatt cgggttactc ggtaagaata ttttcggtac cggaatcgac ttcgacatga
     3781 aattaaaaat gggtgccggt gcatttgttt gcggcgaaga aactgctttg atagcttcta
     3841 tcgaaggaaa gcgcggtatg cctagtccgc gacctccatt ccctgcagta agcggtttat
     3901 tccgtaagcc tactattatt aataatgtgg aaaccttgtc gaatatcccc gaaattatat
     3961 ctcgtggcgc cgattggttt gcttctatcg gaactactac cagcaaaggc actaaggtat
     4021 ttgctctttc agggaaagtt gcactgaccg gacttgttga aattgtaatg ggaacctcta
     4081 ttcgcgatat aatatttacc ataggaggag ggataaaaaa caataaaaaa tttaaagccg
     4141 tacaaattgg cggcccttcg ggcggttgca tcaccgagca aaaccttgat atacagatcg
     4201 attacgaatc gcttattaac gccggtgcca tgatgggttc cggagggctc gttgttatgg
     4261 acgaagacaa ctgcatggtg gatgtggcaa aattttttat ggatttcatc cagcgcgaaa
     4321 gctgcgggaa atgtatcccc tgccgtgaag gaactaaaag aatgcttgaa atacttgaaa
     4381 gtattaccca taaaccgacc aattcaaatc aatacgatgc cctcgaaaga ttcaaaggaa
     4441 ttcttcaact ggaacgtctc ggaaaagtta ttaaagacac ttcactgtgc ggactcggtc
     4501 aaacggctcc caacccggtg ttgagtacct taaaatggtt cagggaagag tacgaagctc
     4561 atatttttga aaggaaatgt ccttcgggtg tttgtactga attgagaact ttccgcattg
     4621 atgttgaaaa atgtacgggt tgcacagtat gtgcaaaaaa atgcccgact aacgccataa
     4681 ttggaacagc caaatctcct cactttatta ttgacagtaa gtgtatcggg tgcggcagtt
     4741 gcgaacaggt ttgtaagttc ggagctgtta ttttaaatga ctaatcacta aaaaattcac
     4801 aaaatggacc tcaggataga agttaataaa caagtgcttg aagcaaacaa aggtgaaaca
     4861 atcctggatg tgctgaacag aaacggtata aaaataccaa cactgtgcca tatgaaggaa
     4921 gcttttccta ccggtgcctg tcgtatgtgc gttgtagaga acgaaaaaac cggaaaactc
     4981 atcacagcgt gttcatatcc cgttgaagaa ggaatgaaaa ttaaaacaca ttcttctcgg
     5041 gttattgaag caagaaaaac tatcgttgaa ttgcttttat cgaaccaccc cgacgattgc
     5101 ctttactgtg taagaaatca gaactgcgaa cttcagaacc tttccgaaga gctaagggta
     5161 cgcgaacgca aaatccgtgg tgtaaaaaat aattgctata tcgataattc cagtgcaagt
     5221 atagtacgtg accctgacaa atgcatctta tgcggaaggt gtgtattcgt ttgcagccag
     5281 gtaatcggag tgtcggctat tgactttgta aaccgcggaa gcaaatcggt tataggaaca
     5341 gcattcaaca gagggctgaa cacatcgagc tgcgtgaatt gcggacaatg tatcatggtt
     5401 tgccccacgg gtgctcttac ggagaaaaat cactttggag agattatgga agcattgaaa
     5461 aaccccgaaa aaacggtagt agttcaatat gctcccgcaa taactgtttc tattgcagaa
     5521 tcgtttggaa tgaaggttgg gaaagacctc aatggtaaaa tgaacgcagc tttgcgcaaa
     5581 attgggttcg acaaagtttt cgacaccagc ttttcggcag acctcaccat aatggaagag
     5641 tcagccgagc tgatcgacag aatttcgaac ggaggcgtgc tgccgatgat taccagttgc
     5701 tgccccgcat ggattaaata tgccgaagaa tttgcatccg actttttacc aaacctttca
     5761 agctgcaaat caccgcagca aatgatgggt gcagttatta aaagtcatta tgctaaaacc
     5821 gctggcatag cacctgaaaa catatattcg gtgtcgataa tgccttgcac tgcaaaaaag
     5881 ttcgaagcac agcgcgaaga aatgactaac aaaggaatta ccgatgttga tgctgtgtta
     5941 accacccgcg aatttgttca gctaataaaa atgttcggca tcgatatgga taagattgaa
     6001 ccggaatcaa ccgattctcc tctgggggca agaaccactg ccggaaaaat attcggtgct
     6061 accggaggtg tgatggaagc tgctattcgt acagcctact tcaaattaac cggaaaagaa
     6121 ctccttactt ttaaagtacc cgaagtaaga gggtttaaag acagaaaaga agccaccctt
     6181 aagataggtg atctcgacgt tacagtggcc gtagtaaatg gacttgcaaa tgcagcagta
     6241 ctactcgatg aaatacgtaa cggaagaaaa gacgttcact ttatcgaaat aatggcttgc
     6301 ccaggcggct gtatcggtgg tggaggtcaa aaaataggat caagtgtgga agaaaaaatg
     6361 gccaaaatga aagccctgta tgatatcgac gaacatgaag cgataaaagt ttcgcacaaa
     6421 aacccggaag taattgaact ttataaggag tttttgggtg aacctctagg ccataagagc
     6481 cacgaactat tgcataccaa gtattcgaaa agggatgttt tgaaataatt tccagaataa
     6541 aatgattaca aagccatgag ccacccacgt agaaataaac tcgtatttac agtaaaaaat
     6601 cgctgtcggg tttgctatac ctgcgtaaga gagtgccctg ctaaagcaat aaaaattgtt
     6661 aacggtcagg cagaagtaat cagcgagcgg tgtattggat gtggaaattg tgtaaaagtt
     6721 tgcagccaga atgctaaagt ctttctcgat actaaaaaag aggtatacgc attaatacag
     6781 tcctcaaaaa aaaccgtagc atgtattgct ccgagttttc ctgctgagtt taccgaaata
     6841 aacgattacc ggatcatcgt tggaatgata aaagcattgg gcttcgacaa agtagtcgaa
     6901 gtttctttcg gggccgatct tattgccaac gaatatgcta aaataattga caatagcaag
     6961 tctacctgca tttcatccga ttgtccggca atcgtgtatt acattgagca ctattacccc
     7021 catctcgtaa catcgcttgc cccgttggtg tcgcccatgg ttgccacagc tagagttgtg
     7081 aaaaaggaat atggagatgt gaatgttgtg ttcatcgggc cctgcatcgc caaaaaagct
     7141 gaatcgtacg aaattgatga agctataact tttaaggaat tgcgtgaatt gtttttggaa
     7201 aataatatat catccagttc ggttttgcca gttaacttcg acccacccct tggagggaaa
     7261 ggtgcaatat tccctgttag cagtggattg ttgcaaacag caaataaaaa cgaagatctt
     7321 acatccggaa aaataattgt tgccagcgga agtaaaagct tcagggagct gattgatgag
     7381 tacgatgaag ggctgatacg aaacagccat ctggagctac tctgctgcga cgggtgtata
     7441 atgggccccg gaatgtcgaa acagggcaag aaatattccc gacgggagtg cataagtaat
     7501 tacgttaaaa acaaattgac agaacttgat actgctgaat gggagaaaaa catcgaaaag
     7561 catagccatc ttgatttatc gaaggctttt gcacctctcg accgaaggaa cgggaaaccg
     7621 tcggaagaaa gcatcagtaa cgtattaaaa acaatgggga aggtcgatcc gaacgatttt
     7681 ttaaattgtg gtgcctgtgg ttataatacc tgtagagaac atgccattgc tataattgaa
     7741 ggactcgccg aaaatgaaat gtgcctgcct tattcaatca gtaaattaca taaatatatt
     7801 caggaattaa acatttcgaa ttcgaagctt aaaaatgccc aaagggcttt aaaacaatcg
     7861 gaaaaactcg caaacatggg gcagctttca gctggtatcg cacatgaact caacaacccg
     7921 ctaggtataa ttaccatgta cagcaacatt cttaaggatg aatccgctgg agatgatcca
     7981 atacgcaaag accttgagct cattgtggaa caagctgatc ggtgcaagaa aatagtaagc
     8041 ggccttttga attttgcccg tcagaatcag gtaaacctca gcgaaaccaa tattgaaacg
     8101 ctatgcaaac atagttttga atcgattgta aacacaggaa acattgtctt cgagttcgaa
     8161 tcgaatctga aaaaccctat ggccatgatt gatgctgatc aaatgatgca ggttcttacc
     8221 aatctcgaga aaaatgctat tgaagctatg cccaaaggcg gaacgctgaa aataaccctc
     8281 aacggggatc agaattcgtt tgagataaac atcagcgata caggtattgg tatcgaaaaa
     8341 agcaatatcg acaaaatatt tactccgttt ttcacaacca aaccgctcgg gaaaggaaca
     8401 ggcttggggt tgccattgat ttacggaatt gtgaaaatgc accgagggca gattgaaatg
     8461 acctcaaacg cagaccctgg caaagggcct actggaacaa attttaaaat tgtattacca
     8521 agattaatta ataacaaaca aaagtagatt gccatgacaa acaaaatgaa aaccattgcc
     8581 attgttgatg atgatatcga ttatttaacg atactcaaaa tgcaggttga gagaatggga
     8641 tttaatgtgg tttttgctga aaaccaaaaa gatggtgaaa aattactgga aaccacaaaa
     8701 cccgatctgg ccattttcga cctgatgatg gaaaatcagg atagtgggtt tattctcagt
     8761 tacaaattaa aaaaaatgta tcccgatgta ccggtgatta ttgcaactgc agtaaccgct
     8821 gccacaggca tcgaatttgg gatcgaaact caggaagaca gaagctggat aaaggctgac
     8881 aaatatcttg agaaaggcct ccggcccgac cagttacacc gcgaaataaa taagttgctg
     8941 aaattataaa aaccaaccgt tatggaaacg cttaaggtat tagttgttga cgacgaacct
     9001 ggaatacgtt ccggaattga aagaattctc cgaaactaca ctgttggctt ccctttcatg
     9061 gaaagcgact tttcattcga attaatcgaa gtggagacgg gcgaaaaagc gatggaaaca
     9121 ttggcaaaag atcgtatcga tattattttg ctcgacaata agttgcccgg cattcagggc
     9181 attgaggtac tggagaatct caataagatg caatacgacg cagttgtaat gatgataaca
     9241 tcgtatgcat cgctcgacct ggctgtaaaa gctaccaaca acggtgctta taactttgtt
     9301 cccaaaccct tcaccccgca ggagttgaag tcggctatgg aaaatataac aaaacacctg
     9361 tacctgaagc gcatgacacg taagatgaat gatgccggaa aagaaatccg ctttcagttt
     9421 ttatcagtac tatcgcacga actaaaatca ccaatcaatg ctatcgaagg atatttaaag
     9481 ataatgcagg aaaaacaggt gggcgacaac atcgatgatt atcaggtgat gatcgacagg
     9541 tcgattacgc gtattaaagg catgcgaaac ctaataatgg atttacttga cctgacaaag
     9601 cttgagtcag gaaaaaaatc aagggaaatt aaaccaactg acatgtatga gcttgccaag
     9661 ctgacaatgc aaagtattga gccattagca attcagcgaa acgtacaaat ccagttcgat
     9721 gccgatgaaa acacctattt tgacgctgat gctgatgaaa tagaaataat tttcaacaac
     9781 ctgctttcga atgctgtgaa atacaacaag gaaggtggca gggtgtattt cacaataaaa
     9841 gcacttaaag atgaaataaa aataagcgtt caagacaccg gtatcggtat gaacgacgaa
     9901 gaattgtcga aaatttttca tgatttcgta agaataaaaa acaaaaaaac tatcgacatt
     9961 accggtagcg ggttaggctt gtcgataatg aaaaaaatgg tcgatttata cggtggtaac
    10021 atcgatgtta caagtgttcc cgatgagggg agtactttta ctgttacctt gccaagataa
    10081 caacaaaata cttttgttgg aatttccagt atctatgata aacaaaattg tccttatttt
    10141 cgttacaaca attttatgca tcttatagca atgtcgtaat cgatgtaatt tgtatttttg
    10201 gcttaatgga attatttgta cgcaaatatg gcaatgcccc ccaacacctg atcattcttc
    10261 acgggatgta tggcagtggc gataactgga caagtattgc cagaaaccta tccgacgggt
    10321 ttacggtgca cctgcccgac cttcgtaacc acggtcgttc tccgcacagc cccgatcaca
    10381 cctatgaatt gctggctgaa gatgtatatg aatttgttac cggtcagcgc atcgagcgat
    10441 tttatcttgc cggtcattcg atgggtggaa aaactgccat gttttttgcc cggcagcatc
    10501 cggaaatgct tataaaaatg gcagccatcg acatatcgcc ccgcacttac catcaattga
    10561 accaactgtc ggagcagaca atttttcatc tcaatcttat aagctatctc atccgctgca
    10621 acgtggaaaa agcacacaac tatgccgaag ctgcaagcat tctcagcgaa ggtgtaccga
    10681 gccagcgcat ggtgcaatcg ctgctgaaaa atctcgaaaa aaccggggat ggaatgatgt
    10741 ggcgtattaa tattgaggcg cttaaagaca atatttacgc tatgggagat ggtcttgatc
    10801 ccgacgactt tatcgataga aaaatcgcag ttcccacatt gtttttgcga gggcttaaat
    10861 caaactatct tccagtcgaa gatgaaaagc tgatccactt tattttcagc aatgcccgca
    10921 ttgtgaacgt tcccgaagca agtcactgga tgcacaccga acagcccgaa gccattgtta
    10981 cagaactccg gcattttttc agtgaagatt gattatcagc agaaacatta ctataaccaa
    11041 aaaaagctat ttttgcggca taaaaacctg taatacaata cctatgatac ttcacagcat
    11101 gctcaaaatt aaactaactc tcctgataat agcaggattc cttctaaccg taaatactac
    11161 tttctcgcaa tctgccagcg acactgccat tgtgtatttc acaaaagaaa tttcgcccga
    11221 aagtgtgatg aaaatttttg aatatgtgaa agataacgtc aaagggaaag tggggataaa
    11281 agtacatttt ggtgaagacg gcaacgagta ttttgtacct gcaagcatgg tggagccttt
    11341 atgcaaagcc tataatgcca cactgataga gacgaatgta gcctataccg ggcgcagaca
    11401 gaaaaccgaa acccacattg aactggcaaa agatcatggc tttacattcg cacccatcga
    11461 cattctcgac gaaggcggca cgctgagttt gccaatcgaa ggaggcagac attttaaaac
    11521 cgccctgatt ggaaagaatt tcgaaaacta cgaaatcata atttatttca cacactttaa
    11581 gggacatggc agagcaggtt tcggaggatg catcaaaaac gcatcgatgg gaatggctac
    11641 tcccgaaggc aaacgcgata tgcacttcaa cgattttccg aaacctgaaa gcaaaaactg
    11701 catgggctgc ggtgcctgtg taagtgaatg tcctgcaaat gccattaccc tcaacccgct
    11761 taccatcgat aaagagaagt gtatcggctg cgggaaatgc atttccacct gcccctatgg
    11821 tgccattacc aagcctgaca atgccgaaca aacaattcgt tttctggaag gtctggttga
    11881 atatgccaag ccggtgattg agcacaccaa ttccattttc ttcaatatta ttatcaatgt
    11941 atcgccaagt tgcgactgtt cacggaaccc aaagcaaccc tttgttgccg acatcgggat
    12001 actggtttcc accgatattg ttgccattga agccgctgcc cacgaccttg tcgataaggc
    12061 acacaactgc gacgatgcct tcctgaaagc caattccgta agtggtaaac atcagatcga
    12121 ctatgccgaa cagctcggca tgggaacgaa aaactataaa ctggtgaata tcgacgagta
    12181 agcaccctgt tgagttcatt atttaaaaaa caattaaatc acattccctg cttggtgtat
    12241 gctaagaaac caattgtaac gtactatatt tgttttcgga caatagcacg gaactataca
    12301 caactcgttc agcacattta attgtgcact taacaattgc gataaagcat ttcgggattt
    12361 accatgttgt gatacaatca aaatatttgt accgaaatca ctatgtaaga aataattgat
    12421 cctctttcta aaggatattc taaaaactgt aattttgtat taatacattc gggaaaatga
    12481 attatactgc ctgcattgaa atgccacgaa aaatgattgt ttttgataag tatgtaaaaa
    12541 gattcaacgc aagctgactt caatctattc ctgaaaaaga tgttttattt aattctataa
    12601 tatttttacg tatttgaaaa cgaaattgtt gaattgcatt gaaaatacaa gaataaataa
    12661 gtcctcgttg caaacgagga ccattattag cattgctcat gctgcgattt gtatcggaat
    12721 aactatgaaa aaagcaatat ttctgatttt aacaatatta tcgataactg tatatgcaca
    12781 ggaaactatt aaatttaatt ataattaccc cttggaatgg acatataact gctactcgat
    12841 ggttgtgcag aataattatt attacttatc gggtactata gggattacga ctgatgagtg
    12901 cgattctgga attatcggat atcatatcaa taaaataaat gatcagggaa taagggacac
    12961 tattgtgata tacagtaaat gccatcacaa aaattatctt ggttggcagg gatcgatggt
    13021 aaattataac gatacaatta ttgttgtcgg attaaaatat attgaagcca atttgaacaa
    13081 agtatttatt gtaaaattaa acccagatct tgatacaatt gctgatttta tttttatgga
    13141 cgataccctg accaaaaggc cactaagtat tacggttgat gatgaaggta attatatttt
    13201 ttgtggtcaa accgacagta gctataatga aattgcaaat cctgcgccgg acgaaaccta
    13261 tacaaaatca tatttgtaca aggtatcgcc atcgggagaa acaatctgga aaaagtcgta
    13321 cccaataacc aattgtagca gcggttgctg gtcgtttttt aggaaagtta tcagcactta
    13381 tgacaaagga tacattgcga taggcacaag tcatgttaat gtacataaaa acataattgt
    13441 aaaagcagat agccttggca atcaggaatg ggtaaggttt tacggcaacc cgaattacga
    13501 aaatccatac ctgatggaca ttgttgaaac tgccgactcg tgcattgtgg tatgtggggc
    13561 ctacgcttac gatgaaatag gcggcggctt atacccctac gacgggtggc ttatcaaatt
    13621 agataaggat gggaacgaga aatggaatag gaaatacagg gatcatgtgc ttacagggat
    13681 gtcggactgg agagatacaa tttacagctt ttatatgggg atttatgaac gcgaaaacgg
    13741 ggacttgatt accatatcgg caacccacag cgacgaaaac ggggtatata ttgcggataa
    13801 gttcaggttg aggtgtttgg atagcggagg aaacatacgg tgggatagag tgattgacag
    13861 cgttggaggc cagactggaa tattgcaagc tcaaagtgtg aaaccaactg ctgatggtgg
    13921 gattgctgta gccggttctg ctgaaatatt ttatatggat ggcacacaat ggacaagcga
    13981 ccaacgcata ttcctgataa aaaccgacag tctgggcaat gatgtggaaa ccatggtgaa
    14041 gcctgttgcg gcacagccca taagtaaatt tgagttggtg tgctacccca acccggcaag
    14101 cagtgagttt tgggttgatt tgccagagga tgacaacggg gatgtgttgg agatatatgc
    14161 taccaacggc agtttggtgt atgagcagcc agcaacaccg ggtttaaacc ggattgattt
    14221 gtgcggggta aagccgggaa tgtttttggt gaaattgaga aatgcgaatg tttttgggaa
    14281 agtggtggtg gaatgagtgc gtcgtttctc cgcccgtcat tgcgagggtt gaaaacccga
    14341 agcaatctgt atttcgacga gcttcccaca ggtacttctt tgacggaatt aatacagaca
    14401 tttcaaaagg ttgtgttgta acgtcttcgt ggatgtggat tgcttcactg cgttcgcaat
    14461 gacgtttgag agcccgtcat tgcgagggtt gaaaacccga agcaatctgt acttaggcgg
    14521 gcgatgatag attcgccttc aggtacggag attgcttcgc tgcgctcgca atgacgtttg
    14581 agaaaggtaa aaatgaaatt taagatctta aaaaattaaa acttcctcag catttcacaa
    14641 cccgcttaac tttttaattt cgttgagttt gttgagtgct tcgagtggag tgagtccgtt
    14701 gatgtcgaga tttttaattt cgtcgcgtat ttgtttcagc acggggtctt cgagctgaaa
    14761 aaagctgagt tgaaagcctt cacggctttt ggcaattttt tccgtaggct tgcttatatg
    14821 ggtctggtcg cggctgcctt cgagctgaac cagaatttgc tttgctcgtt caaccaccga
    14881 gcgcggcata cctgccattt cggctacatg aattccaaaa ctatgttcac tgccaccggg
    14941 tttgagcttt cgcatgaaca gcactctgcc cccgctttct ttcaccgaca cattgtagtt
    15001 tctgatacgt gaaaaagttt tttccatttc attcagttca tgataatggg ttgcaaacaa
    15061 tgtttttgcc cttgcccgcg gattctcgtg aataaactcg gcaattgccc atgcaatact
    15121 caccccatcg taggtactgg ttccccttcc gatttcgtcg agcagcacca gactgcggtc
    15181 ggaaaggttg ttaagaatgc tggcagcctc attcatttcg accataaaag ttgattcacc
    15241 catcgaaata ttatcggaag ctcctacgcg ggtaaatatt ttgtcgataa tacccagcga
    15301 tgctgacaac accggcacat agctaccggc ctgcgccatt aggcatatca aagccgtttg
    15361 cctcagtaat gccgacttac ccgacatatt cggtcctgta atcatcatca tttgctgaac
    15421 ttcagggtcg agatacacat cgtttgtgat atatacatca ccgggcagca gcaacttttc
    15481 gatcacgggg tgacgccctt ctttaatgtc gacaatcaga ctatcgttca tcgacgggcg
    15541 gcaataatga ttttgtatcg ctacggttgc atacgaaata aggcagtcga gctgtgcaat
    15601 caggttggca ttcagttgta tggtgctgat gtagtcgccc atgcttaaca caaggtcgtt
    15661 gaacaaccgc gtttcgattc cgataatttt ttcttcggca cccagaattt tgctttcgta
    15721 ttccttgagt tcttcggtaa tatagcgttc agcgcttaca agggtctgct tgcgcatcca
    15781 gccggcaggc actttatctt tatgggtatt tcgcacctcg aaataatagc cgaacacatt
    15841 gttgaaccca atacgcagcg acgatatgcc ggtgttttcg atttcgcgct cctgcatcga
    15901 aagcagataa tccttccccg atgtggtaat actgcgcagt tcatcaagct ctgccgatac
    15961 tccggcagca ataactttac ccttggcaat caggttcggg gcgtcttcct gcaattcccg
    16021 acccagtcgg ttgcaaatat cggtgcaggg atttatctgg tcggcaatcc gcttcaaaat
    16081 ggcatttcca cttttttcgc aaaggctttt tgcattggta gctgccaaga gcgactgcct
    16141 cagctgcacc atttcgcgtg ggttcacccg caatgcagct actttcgaaa taagtctttc
    16201 gaggtcgccg gtactcgaaa tacatgattt cagtttgtcg gaaaggtcgc tgtcggtaat
    16261 aaaacactcc acaacatcgt ggcggtcgtt aattttttgt cggttcacca aaggcatagc
    16321 aatccagcga cgcagcaaac gcccacccat cgggctggta gtgcggtcga gaatatcgag
    16381 aagcgatttt ccgccttcac tcataggcat aagcagttcc aggttgcgga cagtaaattt
    16441 atcgagccac acatactggt cgcgatcgat acgtgaaagg ctgcttatgt gcccgatgtt
    16501 gcgatgctcg gttatattca gataattcag tatggctcct gcagcaatca ctccatgcac
    16561 catttcgtgt accccgaatc ccttgaggtt tttcacctta aaatgattga gtagcctttc
    16621 gtttgcattt tcctgagtaa aaacccattc gtcaagcgga aatatggtgt actttgaacc
    16681 gaactgctct acaaactggt ctttgaattg cctttcgtac agcacttctt tgggacggaa
    16741 actctgcaac agtttatcga tataatcggc atcgccctga gaaagcagga attctcccgt
    16801 tgatatatcg agtagagcca gtccaaagta ttttttatcg ataaaaacac ttgccagaaa
    16861 gttgttttcc ttgttttcga gaatctgatc gttcagggcg acacccgggg taaccagttc
    16921 ggtaattccc cgctttacaa tagttttggt aagcttcggg tcttccagct gctcgcagat
    16981 agccacccgc aatccagcgc gcaccagttt gggcagatag gtgtcgagcg catggtaggg
    17041 gaaacccgcc agatcgacat acgacgcagc accgttggca cgttttgtaa gtgtaatgcc
    17101 cagaatagct gaactgcggc gtgcatcatc ttcaaatgtt tcatagaagt cgcccaccct
    17161 gaacagcaat attgcatcgg ggtgtttggc cttgatcgca taatactgct tcatcaaggg
    17221 agtttcgtca tttttaacgg gtgcactcat cggtaaaaca ttcggttcaa aattatttaa
    17281 ttaatgcaga aattccgacc aatgttgaaa tcttttaaaa accaaaggga ctttaagtgt
    17341 ttgtcgcata gaaaaccatt tgcagcaatt ggcacagtgc ctgagtttta ttataattgc
    17401 atttgaaaaa cgatacaatg aacgtcctcg acattattat catcattccg ttggtttggt
    17461 tcggatttaa aggacttaaa aacggactta tcaaagaagc aggaagtgtt gctgcattgg
    17521 tgttaggcat attcggtgct ctggagtttt cgggcttcac cgaaaaatac gtccacaatt
    17581 ttacctcggt atcagcgcag tatattccgt taattgcatt tgctgttacc ttcgtggcaa
    17641 tactgattgt agtttattta atttccggac tgctcgataa atttgtaaac gccgttaaac
    17701 ttggatggtt gaacaagctt ggcggtattg tttttggggt actcaaaatt gcatttatca
    17761 taagcgctct gttctacatg ctgcaccagg tgaatacaca gaaaaaatgg atcagcgaaa
    17821 gctatctgaa tacatcgctt ctctacaaac cgattgccgc aatcataccc actgtatatc
    17881 cgtatatacc cgacattgag attaataaac atgagcaaac caaagaagat gaaaagaaat
    17941 agcacatttt tttgttttta aaaaaaacta ttatactttt gcagcgtgaa gataaggaaa
    18001 tagtgacagg ggacaatgtc ccctgtttta ttagaaaaat atgatcgaga aaaaagacat
    18061 agaaaaactt gcgaccggat ttctgggtga aacccagttt cttgttgatg tacaggtaac
    18121 acaggggaat gcaatacggg tatttgttga cgacctgaac ggtcttgata ttgatgcatg
    18181 taaactatta agccgtaaaa ttgagtcagc gctcaaccgt gatgtggaag attttgaact
    18241 cgaagtttcg tcgccggggc tgggaaaggc ttttaaggtg atgcagcaat atgtgaaatg
    18301 tacaggaaaa atgctggagg tcaccctgaa aaatggtata aaaaccgaag gtattctcag
    18361 ggaggtaaat gcagaaaata ttattctgga aacgattaga aaaaagacaa aaaaagatcc
    18421 ggcaccggta cccgaagcaa cgtcaatact gttttcagaa attaaaacag ccaacgaaaa
    18481 attaatcttt taaacataat aacaaaaaaa atggagcaca acaatctgat cgaaaccttc
    18541 tcagaattca aggaactgaa gaacatcgac cgtcccacca tgatcagcgt tctggaaagc
    18601 gtttttaaag ctacgctggt aaagcttttc ggcagcgacg aaaactttaa tatcattatc
    18661 aatgttgacc agggtgactt tgagatttgg agaacccgtg tagttgtaga agacaacgaa
    18721 atcaccgatc cgaacaaaga aattaaactc tctgaagcac aaaaagtgga cgacgattac
    18781 gaagttggtg aagattttat cgatgaagta aaactatacg atttcggaag aagggctatt
    18841 cttgcgttgc gtcaaaacct gcaggcaaaa attgccgaac tcgaaaaaga caatatcttc
    18901 aacaaataca aagaccgcat cggtgaaatt atttctggtg aagtgtatca gatttggaaa
    18961 aaagagattc tgatcctcga cgacgaaggc aacgaactga tattgcccaa aacggagcag
    19021 atacctaccg acttcttccg taaatcggac accgtacgtg ctgttattgc taatgttgaa
    19081 ctgaggaaca ataccccccg catcaccttg tcaagaacat cggagttatt cctagagcgt
    19141 ctgttcgagc aggaagttcc tgaaatcttc gacggactga ttaccattaa aaaaattgta
    19201 cgcataccgg gcgaaagagc caaaatagcc gtggaatcat acgatgaacg ggttgatccg
    19261 gttggagcct gcgtgggaat gaaaggaagc cgtattcatg gcatcgtgag ggaattgcgc
    19321 aacgaaaaca tcgatgtaat caattacaca gccaacacac agctgttaat acaacgagct
    19381 ctgagtcctg ctaaaataag cagtattaag ttgaacaatg aactcaagaa agcggatgtt
    19441 tacctgaaac ccgatcaggt ttcgcttgct atcggcaaag gcggactaaa cataaaactg
    19501 gcaagtcagt tggtaggcta cacaatcgat gtgtaccgcg aaatgaatga ggacgaagaa
    19561 gacgttgacc tcgacgaatt cgctgatgaa attgatggct ggattatcga ccagctgaaa
    19621 gctattggtt gcgatactgc aaagagcgtt ctggagctcg atattgcata cctcgaaaag
    19681 cgcaccgacc tcgaagaaga aacaataaaa gaagttattg ccatattaaa atcggaattt
    19741 gaatagtaat aagtacctga aaaataaata tttacagata attaacacct atggatacag
    19801 agaacaagcc acaaagatta gcaaaaattg ctgctgaact caatgtcggg atcagctcca
    19861 ttgttgattt tttggctaaa aaggggcata aaatcgactc gaatcccaat tcaaaagttc
    19921 cgtttgagct catgcctttg ttgcgcaaag aattcagcga ctccgtaagc ttaaaggagc
    19981 aatcgaacga tatttcgcag cgcacaaagg aaagaaaagc cgtgcttacc gaagtacata
    20041 gtgaaccggt gaaaccggag cacccgaaac atgaagttga agataaagaa ttgcttataa
    20101 aggattcctc tggtttggaa ggtccaaaaa ttgttggtaa aatcaatctc gatgaattga
    20161 acaaaccggt aaagaaacct gttgaacctg ctaaaacgac tactgctgaa cctgaagcaa
    20221 aaaaagtgga gcctgcaaaa atatcagatt cagaaacaga gaaaaaagca gatgatagca
    20281 aaacagagaa aaaaagtcct aaaatagtcg gaacaatcga tctggattcg ttaaaccaaa
    20341 aaacgcgtcc ggataaaaag aaaaaatccg ataaaaaaac cgaaacaccc gagcctgttg
    20401 ctgacgagcc tacagaagcg cctgttgccg aggaacagcc catagttgtt gaaacacctg
    20461 ttgcaaaaga agatgaaccg aataatttca tagaaaccaa atacgataag cttaccgggc
    20521 ccagcgtaat tggcaaaata aatatcgacg actttgttaa aaaggaagaa gatatccgca
    20581 aaaaaagaga gcttgagaaa gtagaacgca aacaggaacg cgaaaaagaa cgtgaacaaa
    20641 agaaaaaaag gaaacgtatc cgcaaagaga ctgttaaggt tgatgtaact aaaaataaag
    20701 agcaaaataa gtttaaaccc aaagaaaccg gtactaagaa aaaaccacaa aaagagataa
    20761 aacccgaggt ttcggacgaa ctggttcaga aacagataaa agaaactctt gcccggttga
    20821 cctcgaccaa gcaaaaatcg aagacatcga aatatcgccg cgaaaaacgc gacatcgtta
    20881 gtcagcgtat tcaggccgaa attgaagaaa ccgaagcaca gaagaatatt cttaaggtta
    20941 ccgaatttgt ttcggtgaac gagctggcta gcatgataca gcttccggtt accgaagtta
    21001 ttgccacctg catgaatttg ggcttgtttg tatcgataaa tcagcgtctc gatgccgaaa
    21061 ccatggcttt ggttgctgac gaatttggct acaaagttga attcgtaagt gccgaagtac
    21121 taagcgatat ttctgacgaa gtggatgatg aagaagagct gcaaccccgt ccgcctattg
    21181 taacggttat gggccatgtc gatcacggga aaaccaaact cctcgactat attaggcaca
    21241 ctaacgtggt tgccggtgaa gccggtggta taacccagca tattggtgct tacagtgtag
    21301 tactcgacag tggaaaacaa attaccttcc tcgacacacc gggtcacgaa gcctttaccg
    21361 ccatgcgtgc ccgcggtgct cagataaccg acatcgccat tattgtggta gctgccgacg
    21421 atgatgtaat gcctcagacc gttgaagcca ttaaccatgc ttcggctgct ggtgttccaa
    21481 tggtttttgc catcaataaa attgataaac ccaatgccaa tccggaaaaa atcaaagagc
    21541 aactggccaa tatgaattat ctgattgaag aatggggtgg aaaatatcaa tctcaggata
    21601 tcagtgcaaa acaaggactt aacgttgacc aattgttaga aaaagtattg ctcgaagcag
    21661 aacttcttga tctgaaagca aatcccaata aaagggcaat cggaactgta gtggaatcgg
    21721 cactcgataa agggaaagga tacacttcga ccattcttgt acaatcgggt acacttcata
    21781 tcggtgatat cattttagca ggaagttata ccggaagagt aaaggctatg tttaatgaac
    21841 gtggtctgaa aattgacact gccggacctt ccaaaccggt gttggtgctc ggtatcagtg
    21901 gtgcacccca ggcaggcgat aaattcaatg taatggaaac agagcacgaa gccaaggcaa
    21961 tagccaataa acgtttgcaa cttcaacgcg aacaaggtct ccggacgcaa aaatacatta
    22021 ccctcgatga aattggtcgt cgtctggcat tgggcaactt ccacgaaatt aagcttatcg
    22081 ttaaaggtga tgtggacggt tctattgaag ccctcagcga ttcgttgata aaactttcga
    22141 atgaggaagt tcaggtaagc gtgatccata aagctgtagg gcagatttcc gagtcggatg
    22201 ttatgcttgc tgctgcttcc gatgccatta taatcggctt ccaggtacgt ccttcaatag
    22261 gtgcgcgccg ccttgccgaa aaagagcaaa tagacatcag gctttactct atcatttatg
    22321 atgccatcaa cgagataaaa gatgctatgg aaggcatgct ttctcccgaa attaaggaaa
    22381 aaattcttgg cgctgccata attaaagaag tgttcaaaat caccaaagtt gggactgttg
    22441 ccggatgtat tgtaactgag ggcaaaatcc tgcggactgc aagaatacgt cttatacgcg
    22501 acggtattgt tgtgtacacc ggagaactcg gttcactgaa acgattcaag gatgatgcaa
    22561 aagaagtggt ttccgggctg gagtgcggtt tgaacatcga gaattacaat gatatgaaag
    22621 tggatgatgt ggtcgaagct ttcgaagaat tcgaaatcaa gaaaaagctg tagatccgca
    22681 ctgcgaaact caatacacta ttgtgtttat aggctctcta aatctgggcg ataagccgct
    22741 gctgctggct cctatggagg atgttaccga tccttcgttc cgctttttgt gcaagcattt
    22801 tggtgccgat atggttttta ccgaatttat ttcgtcggac ggattgctcc acgaaggccg
    22861 taaaagcgtt gaaaaactga atatcagcga aagcgaacga cccattggaa ttcagattta
    22921 cgggcatctt cccgaatcga tggtggaagc cgccttaatt gctgaagcag ccaatccaga
    22981 caccattgat attaacttcg gctgtcccgt taaaaaaatt gccaaccgtg gagccggcag
    23041 tggcatgatg cgctaccccg aacttatggc cgatatcacc gcccgtgttg tgcaagctgt
    23101 gaaaaaaccg gttaccgtga aaacacgcct cggctgggac gagaacagca aaaacattgt
    23161 tgacattgca ttaaggcttc aggatgccgg aattcaggct ttgaccattc acggtcgcac
    23221 gcgttcgcag atgtacaagg gcgaagcaga ctggacgctg ataggggagg tgaaaaatca
    23281 cccggccatt cacattccaa ttatcggtaa tggcgacatc catgacgggc catcggcaaa
    23341 agcggctttc gatacctacg atgttgatgg aataatgatt ggaagggcaa ccgtaggaca
    23401 tccgcgcatt tttgccgaaa taaaacactt tttagagcac ggcaccgaac tgcccccgct
    23461 caatctgaac gaaaccgttg aactggccac cctgcatttt accaaatcgc tggaatataa
    23521 gaaactgccc cgagccattt tcgaaatgcg ccggcacttt tgcacctact tcaagggaat
    23581 tcccgatttt aaagaaaccc gcatgaaact ggtaacctca accaaccctg atgaaatttt
    23641 tgccctgctg caatacatca gacaacgctt tggcgaagga agatgattgt ttttcaaaga
    23701 atacagaagg cagaagacag aagacagaag gggcggctgg caatgaaaca gcattcttct
    23761 ccattcgatt tccccggagc agcagttttc tatcgcccaa cgaaaagccc cttctgtatt
    23821 ctgtctcctg tattccacaa taaaaatagc tttttcatca atcaattcac tatatttgct
    23881 acatgactaa cgagataatc atttatcagt acaacgaaca gtcggtacgg ctcgaagtga
    23941 ggattgagga ggataccgtg tggcttacgc aagcgcaaat ggcggagctt tttcaaacaa
    24001 ctccccagaa tattactatt catttacgga atatttatca ggaaggtgaa ttgaacaagg
    24061 aaggaacttg taaggattac ttacatgttc aaaacgaagg cgctcgcaag gtgaaaagaa
    24121 ctattcaact ttacaatctc gatgtgatca tttcggcagg atacagggtg aagtcaaaac
    24181 gtggaaccca gtttcgaata tgggcaaatc aggtactgaa agaatatttg ctcagaggat
    24241 atgccatgaa caaccgtatg agcaggattg aggatcgtgt tcattcgttg atgaaaaaag
    24301 tcgacgacat cgaccttcag ataaaaacca gcctgccgcc cacccagggc atttacttcg
    24361 acgggcaaat ttttgatgca tgggtgtttg tttcgggttt ggtgaaatcg gcaaaccatt
    24421 cgctggtgct tatcgacaat tatgtggatg agtcggtgct caaccttttc ctgaaacgta
    24481 atgccagcgt gagtgttcgc atttataccg cccacatcac caaaaccctg caaaccgacc
    24541 tcgaaaagca caacaagcaa tatgcgccaa tcgaaatact cagctacacc aaagcccacg
    24601 atcgttttct gattatcgat aaccaaactg tgtatcacat gggcgcctcg ctaaaagatt
    24661 taggcaaaaa actctttgct ttctcgaaaa tgaacctgaa tgctcaggaa atattaaaaa
    24721 caattcaggc catatgatga agaaagctat tatcctattg aaagcggcag ccttcagtat
    24781 aatgctgctg tgcattgtgt ttcaaagcaa agcccaaaca tgggatacaa ttcctggctt
    24841 agatattcat tctatggctt taaattttac taactataat gagacgttaa ttatcgcagg
    24901 tgcttttaga gatgatttgc catacaccca cagtaattgt gtaataggtt gggattcaat
    24961 aaatttatat tcttttcaag gaattacaga ttatggttct gctgcatatg cgactatggt
    25021 ttacaaaaac cagctttatt taggaggtat gtttggaagc cctagtaatg atactgatat
    25081 agaaaagttt gctgtatgga atgggtcaag ctgggagcag gtaggcaatg ccataatgag
    25141 ccctcagggc agggttacac atttattgga atatagagat acattgtatg ttggcggtga
    25201 ctatggagcc attaattata atcctaattt gtgggattta gttaaatggg atgggagcga
    25261 tttttattca tgcaggccct atgctgcaac ttgctttgga ttggctgtct ataatgatgt
    25321 actctattcg ggcatatatg gagcatttat aaacaatgac ggagttcccg ttgaggggtt
    25381 cacaatgtac ggtgagaata actggacttc ggttttcgat agagatgggg ttatgttccc
    25441 tggctgtgtt aacagcctgc tcactgatac cattaacaac gatttatata ttgggtgtgt
    25501 tcaggtttat cgttacgatg gggaatactt tcatcaaatt gcacaaatga atggacaagt
    25561 taatagacat gcaatgcaga tgtatcatgg agacttgtat attggaggca tgtttactca
    25621 ggtggatggc attccagcca aacatctggc caaatgggac ggggagcaat ggtcggaacc
    25681 cgggggggga gtaagcaatt caattgagtc catgtatgtt tataaagatg agctgtatgt
    25741 aggcggatac ttcacacagg taggtgggta tttaccggct tatggcgtgg caaggtatta
    25801 tgagcctgag gtggaagact gccactggtt tcgcccacgt attcaaacag caggctatca
    25861 ggacacgttt tacatcactc aaagccagcc atacgtattt gtaaactttg taaacaacaa
    25921 tgcatatgcc gagagctggg agtgggattt tggagacggt ttcagtggta gcggtagcaa
    25981 agacatagag catggctact atcaacccgg tgaatatacg gtgctggtgg aggtaacaca
    26041 ggatggttgc actaaaacag cagaaaaact catcgtaata gaagaccaca caggaggcat
    26101 taccagtaaa actgggaatg acgagataac actattccca aacccgtgct acgataaaat
    26161 ttgggtgccg tatttttgcc cggcaggaga aacctaccga gtagaagttt gtgatataac
    26221 aggccgtgtg cttataagcg accagcttaa agcaggtaac aacgtgtatc cggtgttcac
    26281 atctatgcta agtccgggaa catacctgat tcgaattgcc aatgaaaaag gaagtgtaaa
    26341 aaccaaacgc tttgtaaaac agcaataata tgaagaaaat catagtttta tttaaaacaa
    26401 caatcataag cttaatgctg ttgtgcagcc ttttgcaagg caaggctcaa acatgggata
    26461 caattattgg aatggcacta aacgcacgta cttattgctt cattgacttt cacgacactt
    26521 taatattggc gggtaactat tcaaatggaa tgcctttgac taatagtaat tctgtcattg
    26581 gatgggatgg gattaatgta tattcctatc ctggtaatat ccaatctaca cctgccaaag
    26641 ccttgggact ttacaacggt cgtttatata ttgggggttc attcgatgat attaccaacc
    26701 ctgatgacat ctcaaaatta gccgtttgga atggtacgga atgggaacgg gtgggcactg
    26761 ctgtggtggg tgccggaacg atatactatt ttcaggtgta ccgcgataca ttgtatattg
    26821 gaggtaatta tggcataaat tttaatagta ttaatgatgg ctatttggtt aaatggaacg
    26881 atactgcgtt ttatagctgt tctacttatg gaagtgcttc atttggaact catcctacag
    26941 gcatagcggt ttataacgat gctttatatg ccggtttata ctcatgcttt tcggtagggg
    27001 gtggccagta tgtttgtagt ctggggaaat ataacgggca gacctgggca aatgtttatg
    27061 ataaaaatgg caatcaattt cagtcgggag ttagtagttt gcttaccgat accataaaca
    27121 acgatttgta tattgcaggt atttctgttt ttcgctacga cggagagtct tttcacctta
    27181 ttgggacagc aggttctcag gtgaatggtc atgcgatgca gatgtatcat ggagacttgt
    27241 acattggcgg catgttttct caggtagatg gcattccagc caaccatcta gccaaatggg
    27301 atggggagca atggtcggaa ccagaggggg gggtaagcaa ttcaattgag tccatgtatg
    27361 tttataaaga tgagctgtat gtaggcgggt atttcgactc tgtaggtggg tatttaccgg
    27421 cttatggagt agcaaggtat tacgagcctg cggcggaaga ctgccactgg ttccgcccgc
    27481 gcattcaaac ggcaggctac caggatacgt tttacatcac ccaaagccag ccatacatat
    27541 ttatagactt tgtaaacaac aatgcctacg ccgagagctg ggagtgggat tttggagacg
    27601 gtttcaacgg tagtggcagc aaagacatag agcatggcta ctaccaaccc ggggaataca
    27661 cagtgcaggt tgaggtaacg caggacggat gcaccaaaat cgccgaaaag ctgattgtga
    27721 tagaagacca cactgggggc gttgccaata aagcgggtac cggggcaatt accgtatatc
    27781 ccaacccggg cagcggactg gttgtaataa aaaccaacaa cggcagcgcc ataggaagaa
    27841 ttcgcatcag cagtacatta ggaaagcaaa tgtacaaagc cgaaatcagc aatccagaaa
    27901 tcagcataga cataagtcaa tggaatgcag gaacttattt ggtggaatgc aaagaacacg
    27961 gcgtgatgaa gctggtggtg gaatgagttg gcagaacaca gaagacagaa ttcagaatac
    28021 aggagacaga atactgaagg ggcaactggc aattatcaaa cattcttctc tggctggatt
    28081 tttagaacct tctcggagca gcagatttct ttagcacaac gaaaagcccc ttctgtattc
    28141 tgtctcctgt attctgaatt ccccttaaaa aaatagcatt cttctccaga acccctgcct
    28201 atgtttcgat taactcaaca tgacagggga ctcagcatgt aagggcggtt tttaggatta
    28261 tcacctactc tttagctcct gtacttttaa taaattaatg ctgtcgactg cctgtaacgg
    28321 atttccgaga tggtaaaaat caaccacaat gaaattcgga tatttatccg acaatctttt
    28381 acaatcaaaa caccggtgca aaattacatt catgccattc gcttttttcg attgagttct
    28441 gctaccaatt cctaagccac cgttaatgaa atgattgaga acgaacagcg gattatcgga
    28501 ctttcccctg ttagtggaac acttaaaatc gcacatccga tttaccgaaa aatgtgtttc
    28561 agtgaaaaag tcccacatag taaaaatcct tgcctgccgg ttcatggtgt catttacatc
    28621 attgaaaacc aacagtctgg tgttatcatc tattaattcc tgaattgtcg gccattccct
    28681 gatggagtcg gcataatact ggaatttact cagccctgtt ttatgtaaat ccgactgaat
    28741 aaggtaggcc ggagcatagt tttcgagaag cagggtaatc acagcattcc tgtttacttc
    28801 gagaaatatt tctacttcct ttaaaacctc agcaaatggc tctttgccaa gcatttgtat
    28861 gctgtgatac acatataacc ggcctttcac ttcatacaca tcaagcatta gggcacgaac
    28921 gccgtcattt agttgctgct gaataccata cttctggttt ggaagcaaaa atccgttacg
    28981 tgagcaattg aaagagttat gtgtcgtaag aaagcatgtc ttattatagg gtagttctgt
    29041 tattaattga caaaatacca tttgattgaa tagcaacatc atgcaaaatg ccaccataac
    29101 tagacctttt acaggacttc gcttgagggc agatttaaaa atattgcacc aatgttgctt
    29161 aaccatactt gtaaataggt tatcaacgta aatgctcttt atcaccggta tttgtcagcg
    29221 ttagttttcg attaaatttt agtctaatta ttataaacgg tggtgcaaaa taattaatgc
    29281 acattttatt tttttagcgt aaatttctgc gtcattgaat atcggtggtt tttaatttcg
    29341 acaatatata tccctgcggg ttttccggca agcgaaaatt gtgttttatc cgattgtata
    29401 acctgtgtga gcaccacatc gcctatggca ttgtataaag ttaacctatt attcatcaac
    29461 aggttacctt taaatataag ctcaaaactt cccttttcag aaaggtcatt tatagtaaat
    29521 tcaatcgtat tttcgagtga aggcaggtat tttttgtcgc aatacttatt gttttctccc
    29581 agaaaacgga tggtttgata aaacacaggc caccagtatt cgcacgggaa tgtatcgtag
    29641 ttgagttcgc tgataatgat attgccgcag gcatagaaac aatgtggctg ccccagataa
    29701 gggtcgagca cagtatgcgt ccacccaagt ttatcgcaat gctctttcaa cgattttgaa
    29761 ccatacaaaa acacattgag gtcttttccg agagtgaaat tcaatgcata atccttatcg
    29821 tagggcacca cctcgtcggc agtgccatga ataaataaca ccggaatttc ctcatcctta
    29881 tcgatgattt caggatttag ggcggcaccc caaaggctaa caactcctgc tacagcgcat
    29941 ttatttttac gaaagcctcc tgtacagtcc aagcatccca aatcgtggtt gttggcgcca
    30001 tatccggttt catatgtatc ggccgatctc tcatcaccgg tcataaatgc tgtattaatt
    30061 gcagtaattg ctccggcact gttccccaaa aggaatatac atgttgtatc gatatgatat
    30121 ctttcatgct gattgataaa atatcgcact gcggccctgc aatcttgtgt agaacgatac
    30181 aatgccctaa taacaccttc gtcggcaccg aaaccttttc cgctagtaag cggattaaag
    30241 ccaagccggt aattaattgc tgcagcaaca taaccataat gcgcaagact gtcgcaccat
    30301 gcaatcatat caggacgctc ttttgaccca gcaagaaatg cccctccaaa taaacaaatt
    30361 acaagcggtc gtgctttcat gttatcgcct gaagggaagt aaacatccat tcttaacggt
    30421 tcagggttat caatcccgaa ccccataaaa tcgaaaccat cttcaatggc aaaaacgatg
    30481 tctctttcga tgttgatatt ttcgaatgac tgcgacacaa accgatgctg cgcttttaaa
    30541 accagagaaa taaaaatgat tgcaacgaaa agcaatatct tatgaaaaaa acaacaacac
    30601 atttttcgca attaaaccac aaatttaaaa tttaatcgtt atagtgtttg tttaccatat
    30661 ttttacagaa gaaattaaac agcacaccat cattttttta agttgcagaa tttttatgca
    30721 ggaggccgca ataaacaagc ttatttttta atttatttgc attagcatgt acaaattatt
    30781 tcgaaatatt accattacac tgatttttgt tgcaggagtc attaattttg cttcagctca
    30841 gctcatcagg atgaacaacc gtatggtaat agccggaatt gttgttgatg ctgaaacaga
    30901 agcttcgatg ccttatgtaa atgtttatgt taaacatacc cggactggta caattaccga
    30961 tactgcggga tattttgtac tttttgtcaa tattggcgat accatcattt tttcatcgat
    31021 aggttacgaa aagcttcatg tgctcgccat cgacacattg ggcgacatca gccggcccgc
    31081 ctgggtaagg ctgaaacgaa aagtttacga attgcaaact gttgatataa ttgcacttcg
    31141 gagatatgaa caattcaagt atgaatttac tacaatggta ctgcccgaaa atgattacac
    31201 acatgccgcc cgcaattttc cggttagacc tccggagctg gactactata cccgccaggg
    31261 ggcatcgggg ttcggactga ttttcagccc tatctctgca ctttacgacg ccttcagcaa
    31321 agaaggcaaa gaaaggcgaa agctggagga aatcgaggaa caggatgctc taaaaaaaga
    31381 agttgcaaaa aaatacaacc ctgcaatggt gatgcgcatc accgggttga atgaaccgga
    31441 tgccattgcc tttatgaatt ggtgtcagct tccagacagg ctgattttaa atgcaactga
    31501 atatcagatt atcgaaatta tcatgatgca gttcgataaa tacaataaat aaactattct
    31561 atctttgata attgtcaaga tattattatc attgttatac ttttttcacc catggttctg
    31621 taatgtgcaa ttataacata taaaatgcct gtacttttta aaccccaaac aacaaaacag
    31681 gtaattattt tactgctaat caacatgttg actatttctt tttcaattcc cggaatgtcg
    31741 cagtcttccg atacttctcc cgtggtatat acccatacgg tgaggcaaag catgcatgcc
    31801 cgcatggtaa aaggaatagc acggcttttt ttaccgaaac acaatctgga gaaaaaactg
    31861 aagcaaaaga actttgtttc ggaacccgca cagatacccc gaactgtgct tcgtaatttt
    31921 ataattgatg aatatgatat ttgtggccga aaggtgtatt gcatttctcc aaaaaatgaa
    31981 tcctgtggga aaagcattct ctttcttcat ggtggggcat atatcaacaa tattttcagg
    32041 cagcattttc agtttgctgg aaaaattgca actctcaccg gttgccgggt gattatcccc
    32101 gattaccctc ttgcaccatc ggcttcatat agcgatgcgt atattatgct aactgctgtg
    32161 tatgcaagct tgttgcgaga aacggcaaac gaaagcataa ttttcatggg cgattcagcc
    32221 ggtggtggac tggcactagc ttttgcccag tacctgagcc agaataaaac tccactacct
    32281 tcacaactga tactactatg cccttggctt gacgtaagca tgacaaatcc agaaattcaa
    32341 agtatcctaa aaaaagatcc gatacttaga actcccgggc tagtaatagc aggaaaagta
    32401 tgggcaggtt cgtgcgacac aaaaaaccca atgattagtc cgatttacgg tgatttagcg
    32461 aatttacctc acattagcat gttcattggt acatacgaca ttttgtatgc cgattgtaac
    32521 aaactatcca ctaaattaca aagttgcaat gtgcataccc gaatgtttgt atttccaaaa
    32581 atgtttcacg actggatgtt gtacccaaac cttcgcgaag ccgatgcagc cattaatata
    32641 attcaaggtc ttatcacaca gggtcccagg tatgatactg aaagtaaatg aatacctccc
    32701 tgaataaatg cttagaaatc aaattgtttc aaattttctc agaagtttac ccctggtcat
    32761 tttcacttga tattgtcata agaaagattc actgaagcaa tatggaaagc gcagatcgtc
    32821 acaagaaaag attattcatt tgatttacaa caacttaacc cgttgaaata aaatatttgt
    32881 aaaattgttg atttttcaaa ataattatat ctttgcagtc cgtttggtag aactacctaa
    32941 tagggcaaaa ttagtagcaa tttaaaattt tattgagtga acactttgag ttttaaaaca
    33001 ttgtcagcca acagatcgac tgttcagaaa gaatggttgc tggttgatgc agaaggtcag
    33061 acccttggaa ggttggcttc gggagttgca tatttgttac gcggcaaaca taagcccgat
    33121 tttactcctc atgttgattg tggcgcaaat gttgtggtta tcaatgccga aaaaatcgtt
    33181 ttcaccggca agaaactaac cgacaaagaa tacattagcc attcaggata tccgggcggg
    33241 caaaaaattc agacggcaga tgagctcatg gcgaaatacc ctgaaagaat catcgaaaat
    33301 gccgtaaaag gtatgctgcc taagaataag ctggcaaatc agctattccg caacttacac
    33361 gtatgtgtag gaagcgaaca tcctcatgca gcccagaaac caaaaaaaat tgatatcaac
    33421 gaaattaaat agccaacatg gatacagtaa atgcgatagg cagaagaaaa gccgcaattg
    33481 caagaatata tcttagcgat ggaaaaggta atattactat taatgaccga aaaattgaag
    33541 agtatttccc tttactccaa tatcagtatc tcgccaaaca agcattactg gttaccggac
    33601 tactcgaaaa attcgacata aaagccaatc tcgaaggcgg cggtatcaaa ggtcaggccg
    33661 aagctgtacg ccttggcatt gcccgagcca ttatcaaggc cgacgccgag caacgcccca
    33721 tcctcaaatc gaatggtttt ctcacgcgtg atgcccgtgt ggttgagcgc aaaaaaccgg
    33781 gacgcaaaaa agcccgtaag agattccagt tcagcaagcg ttagtattga tagcttatca
    33841 ggtttagaat ctaaattatc aagactatcc tatttgcgat actacttggt aattgcttaa
    33901 aataaaagaa cgtaaactta aaattaaatt aaaatgccaa gaatcagttt tgatgaatta
    33961 ttgaatgcag gtgtgcattt cggacacctc cgtcgtaaat ggaatcccca catggctccc
    34021 tatattttta tggagcggaa tggtattcac attattgacc tgtacaaaac cagtgcaaaa
    34081 atcgacgagg ctgccaacgc actcaagcaa attgccaaat cgggaaggaa aatacttttc
    34141 gttgctacca agaaacaggc taaagacatt gtggctgaag aagtaaaaaa catcgggatg
    34201 ccttatgtta cagaacgttg gcccggtgga atgctcacaa atttccccac catcaggagg
    34261 gctgttaaaa agatgacttc catcgacaaa atgattaccg acggaacttt cgacaccctt
    34321 tcaaaacgcg aaaaactgca ggtaacccgt cagcgtgcaa aactcgagaa aaaccttgga
    34381 agtattgccg acttgtcgcg cctcccttct gccctttttg tggtcgatgt aatgaaagag
    34441 aacatttcgg ttcgtgaagc acagcgtctg agcatacccg tatttgccat tgttgacaca
    34501 aattccgatc ctaatggtat tgattttcca atcccagcca atgacgatgc taccaaatcg
    34561 atacaactga ttgttggtac tatgtgcaat gcaattaaag aaggtttgga agaaagaaat
    34621 caggaaaaag acaaactgat ggacagaaaa gccgatgatg aaatcgaaga agatgaagat
    34681 gatgaaatca tcgaaaacaa atctaccggc aatctcattg ttgaagaaga agaggaagtt
    34741 gctaagactg ctccgaaaaa aacattagga cgcaaaaaaa ttagtaaacc aatcatcaaa
    34801 acagaagaat aaactattaa aataaataat aaaatggcat taattacagc agcagacgta
    34861 agtaagttac gtaaaataac cggtgcaggt atgatggatt gtaaaaaagc tcttaccgaa
    34921 gccgacggaa atttcgaaag ggctcaggag ctcatccgcg aacggggact tgcaatagcc
    34981 aataaaagag ccgatcgtga agcctcggaa ggagctgttt tgtcttgcag cgctaacgat
    35041 aaaaaagccg gtgttgttat tgcactgaac tgtgaaacag atttcgttgc aaaaaatcag
    35101 gactttatag ctcttgcaaa tgatattcta aaacttgcag tcgaaaaact tcctgcggat
    35161 ctcgatgcac tgaaatctac taaacttggt aataaaactg ttgaggaaat tgtactggaa
    35221 cgcagtggta ttaccggcga aaaaatggag ctcgcttact acgatttcgt aaaagccaat
    35281 tatgttgctt actatatcca tatgggcaat aaactggcta ctattgcagg ttttaatcaa
    35341 cctgttgatg agcaagtcgg gaaagacgta gcaatgcaaa tcgctgctat ggatccggta
    35401 gccgttgata aagacgacgt tcctcagcat ataattgaga aagaactcga aataggccgt
    35461 gaacaagcac gtaacgacgg aaaacccgaa aatatgcttg ataaaattgc acttggtaaa
    35521 ctgaataaat tcttcaaaga ctccacactg ttgaatcagg aatttgtaaa ggatagtaaa
    35581 aaaactattg cacagtatct gcatgaacag gataaaaatc tcacagttac agcgtttaaa
    35641 cgctttggac taaagaacta atcatattga ttttaattta aaagacctga aattcaggtc
    35701 ttttttttat ttttgcgaca ataaaagcaa caacatgtct gcacctgaat acaaaagagt
    35761 actgttaaaa ctttccggcg aatcgcttca gggaagtcaa acatctggta tcgatgaaaa
    35821 aaaactgatg gaatatgctg ttcagattcg cgaaattcaa acacttggaa tcgaagtcgg
    35881 gatagtaata ggcggtggta atatcttcag gggaattacc ggaacccaaa aaggcttcga
    35941 ccgtgttacg ggagatcaga tgggaatgct tgccacagtt atcaattctc tgtcgttaca
    36001 caatgcacta acctcgatgg gtgcaaaagt aaaactattt actgcactta caattgagtc
    36061 agttggggat cgtttctcaa aacccaaagc tgtcgaagca atgaaaaatg gccatatatg
    36121 catttttgcc ggaggaaccg gcaatccgtt tttcagcacc gatacggctt ctgcattacg
    36181 tgctgttgaa acagactgcg atgttttgct taagggcacc agggttgacg gtgtttatac
    36241 tgccgatcct gaaaaagacc cgaccgcggt gaaatacgac accctcactt tcggggaagc
    36301 ctacgaaaaa ggtctgaaga ttatggacct aaccgccttt accctttgcc gtgaaaacaa
    36361 actgaaagta attgttttcg acatggacac tcctggtaat ctgaaaaagt tgctttcagg
    36421 aaataaaata ggcacattag taagcattta atttaaaaac cgactattta tgaatacaga
    36481 cgatgttcag ttttatctag aatcggcaaa agaagaaatg aatggtgcaa tcgagcatct
    36541 gaatcgtgaa ttttataaaa taagagcagg gaaagcttca cccgcaatgc tcgaaagtgt
    36601 taaggtcgat tattacggta ccattagtcc gctttcgcag gttgccaact tgtcgagtcc
    36661 tgacgcaaaa actatcttca ttcaaccctg ggacagggga gttatcgaag ctattgagaa
    36721 agcaattcag gcggcaaacc ttggatttaa tccttcgaac aatggagagg tgatacgcat
    36781 tattgttcca cccctaaccg aagaaagaag gaaacaactt gtgaagcagg taaaaaacga
    36841 aggagaagct gccaaggtaa ccattagaaa tcaccgcaga gattttatcg aggagttcaa
    36901 aaaacttaaa aaagacggac ttgctgaaga tttggcaaaa gatgcggaag ccaaaattca
    36961 caaaatgcat gaagactata tccgcaaaat cgatgagatc atgctccgga aagaaaaaga
    37021 gattatgacc gtataagctc tgttttcggt gcgtattaat tgtattatca actatgccaa
    37081 ttaaaaccgg ttcacatgaa ccaaatatgt atcacatgaa aaaatcagta ttactattca
    37141 tcgcacttgt tgtcagccta ccaatatttt ctcaggacaa atcggactgg gttgcaggtt
    37201 atcccgatgg gtgtaccagt attactgctg gtaaaatggc gaccaccgat ggttcggtat
    37261 ttacttccca cactgacgat agccatcgca cacgctccga aatcattatc gttttagggc
    37321 aggactactc aacgggaagt aaaatgcctt tgtataagcg aattgcttgc gataccactg
    37381 caatgccatc gtatcagaat ataagaattg gcgagatccc tcaggctaaa catacatatc
    37441 attacataaa cacggcgtat ccatgtatga atgagtttca gcttggaatt ggagaatcga
    37501 cttttggtgg tcgcgattgt ttacagtcgg atgccgggct tattgactgc cagcgtttgt
    37561 gtcagctgat tctggagcgg tgcaaaactg cccgtgaagc cataatactt acagacgctc
    37621 tcacaaaaga atatggatgg aacgactatg gtgaatgcct tactatttcc gataaaaatg
    37681 aagtctggca tctcgaaatt gttgggcccg gtaagggcga agtaggcgca atatgggccg
    37741 cacagcgcgt tcccgacgac cacatttcat gcaactgcaa tgcaagtcgt attctggaaa
    37801 tagacttaaa taacaaagaa tatttcatgg cttcagataa cattttcaca atggctcgcg
    37861 acagtgcctg gtggaaccca gagaaagaag tattccggtt tgcctatgcc tatgctccag
    37921 aatcgcgtaa atcacttgcc agtcgccgta gggagtggcg tattttcgac ttacttgccc
    37981 cttcgttaca tcttgatcca aacgatgaaa attatccctt ttcggtgaaa cccgattctt
    38041 tggtgtcgat tgacaagttg attgtcgttt ttcaggatta ttatgaggga accccgtacg
    38101 atatgacaag atgcattacc actaccgata aagatggaaa aacaacgatt tccccacttg
    38161 ccaatccttt catgccatac gacgaactat cgctacataa tgtgagcgga ggctggtata
    38221 gtacaaatca tgacgggagc atacaatttc ttggtgaaag aaccattgcc cgctggtata
    38281 caatgtacgg aactctgatt caatcgcgtt cgtggctgcc agacgaaatc ggaggcgtag
    38341 catggatagc cctcgacaac attgccacct cggtatatgt tccattatac tgttcaataa
    38401 ccgaccttcc acaatcgtac aagactccgg gccggacaaa aggctacact ttgcaatctg
    38461 cttggtgggt gtttaaccga cttggtactc ttgcagccca acgttgggga gacatgagac
    38521 acgatgttga tgcagcttgg aaaccctggc aaaaagaact catcaggaat caaaacaata
    38581 ttgataagtt cgcgcaaaac atgaaatcaa caaaaaaaag ggttgaattc ctgacgcagt
    38641 attcaaacga tcaggcaaac aaagccgtga caaaagctat tgagcttggc gattttctgt
    38701 ggacaaaata cgacgaaaaa ttctaacctg ctgagaatca ttcatcagcc tcattgtaaa
    38761 taattttctt tttactacat gcattttcaa ggatttcgag ttctcctgtc cagttaaaaa
    38821 cgccatcggt tgcttcgtaa gcgtaggtgc cgatagggat atcgtaaaaa ttcgcacagt
    38881 ttgcattccc gcactcagga gcaagatcga aattttcgga aatatgctga gttggaccat
    38941 aaaatgttac cgttatatca tcgtaatgtt cacttttcca aaacattgcg ctacccaatt
    39001 tgggtaggac attcactgtt ttaattattt cgtcggtaag gtcaccctta cgcgaaaaag
    39061 cttcaagttt aaagtacagg agccccgatg aagtattggg gattacatac gtcgcattgg
    39121 tttcagtact tatctgattg tcgggaaatg tccatacata atgatgtgaa tcgaccgatg
    39181 tatttgttag cactacagtt tctccggcgt tgtaaacggc cttatcggta acaaaacttg
    39241 cgtggggggt tttgttgcat cctgcaaaaa ttattgcagc tacaaacatc aatgcgatta
    39301 aatgtgtctt ttttaccatc ataatttacc cattatcagt atgtaagtac atgtatttca
    39361 gacacaaaaa taatagttgt aattcaatga taaaataagg ttttgaaaaa ctattgaaca
    39421 atttttcgat ggtaatcaac aataatctgg ctactgcctg tttgtccgtt tcatgatttc
    39481 tgtttggcgg gcaatggatt ctttgtgtat aagttcgagc aattgcttga tgaactcttt
    39541 cgaaagtccg gctttattgg attgctccag acgggactcc aaaattctgc cccagcgttt
    39601 taactgcagt acggccacat tgtttttctt tttgtactct ccaatctcat caacatattt
    39661 catccgatgc gacagcagct caagtagttg gaaatcgatt acatcaattt tttcacgaag
    39721 gtcttcaagg tgatttttga aatcggcatc ctccgactga acattgcgag ccgtaagaga
    39781 accgatcaga gcaaaaaact ctgcgggact cagctgctgc ctactgtcgc taagggctgc
    39841 ttttggactg atatgcactt caaccataag tccggcaaaa ttaagatcga aagcacgttg
    39901 tgcaatgtcg ctgatgtact tcttattacc ggcaatatga cttgggtcgc aaatcatagg
    39961 gatttcagga aatctccgac gaaactctat aggaatctcc cacattggag tgttgcgcaa
    40021 ttcacttttt tcgaatggga aaaatccacg gtgaatggca gcaatatctt ccaattgatt
    40081 gtcgagcaag cgctccactg taccactcca gagctcaata tcagggtaca cagggttttt
    40141 taccattaca ctaaaattgc tgcctttcaa agcgtctgca agtttttgaa ccgaaaacgg
    40201 attcgataca gtgcgggcac caaaccaaac aatctttatc ccgtacttat acagcatttc
    40261 gatgtgttca ggctcagcaa cttctactgc cacaggcagg ttaaaattat cttgcaccat
    40321 tttcaaccat ggcaatgcct ctgctcccct gccctgaaag ctgttcggtc gcgaacgggg
    40381 tttccaaacc cctgccctga aaacttttac atcaggtatt gaggcaagtg cctgagccgt
    40441 ttcaagcaac tgtttttcgg tttcagcact gcaagggcct gctattagca aggggttatc
    40501 ggtaggttca aacgttttca caatgcaaaa atacctaaaa taatcgaaaa agggtgcaga
    40561 agttttcgct ggtagttccg atgttgttta aaaacttaat accacaaatt ttcatttttt
    40621 tagcttgtta ttattctata gctttgtttt ttgaattatt tttacataat caacagtttt
    40681 aatttatggc agcttttcgg gttctaacat taaatgaagt gataattcag aaacagtcgg
    40741 cattcccgta tgccaagggt gagttatcac gactactgag tcacattggt ctggcggcaa
    40801 aaattgtaaa taaacaggtt aacaaagccg ggctggttaa cattttgggc gaagccaaca
    40861 aaataaatgt tcagggcgaa tcgcagcaaa agcttgacgt atttgccgat gatgtgttca
    40921 aaaaggcaat gcaggcatcg ggaattgtat gcggaatagc ttcggaagaa aacgatgaaa
    40981 ttatcatctt cgatgacgac ctttcgcttg agggtaacta cgtggtttgt atagatccgc
    41041 tcgacgggag ttccaacatc gatgtgaacg tttccattgg ttctatattt tcgatttatc
    41101 gtcgactttc cccggtaggg tccaaagccg aactccgcga ttttctgcaa cccggcatca
    41161 agcagatagc tgccggatat gtaatctatg gttcatccac aatgcttgtg tacactacag
    41221 ggcttggaac aaccggtttt acttacgatg catcaattgg agaattttgc ctttctacta
    41281 cgtatatggt taccccgaaa agcggtaaaa tatattccat caatgaaggt aactatgtta
    41341 agtttccgca gggtgttaag caatacatca aatattgcca ggaagacgat aaagaaacag
    41401 gaagacctta cacttcaaga tatattggct ctctggttgc cgattttcat cgcaacctca
    41461 tcaagggagg aatttatatt taccccacta ctgccagcag ccccaatggg aaactacgac
    41521 ttctatatga atgcaatcca attgcttttc tggcagaaca agccggcggt gctgccaccg
    41581 acggcagggg gaaacgtatc atggaaatta ttccgacgag tattcaccag cgcacaccgt
    41641 tttttaccgg ttcgaaaaat atggtggaaa aagcagagca actgatgcgt gaattcgggg
    41701 actaacattg tgaccttcgc tgctttcata aggtttaaac ttgtatttgt atttatttca
    41761 tgtatttata taagcattgc tgtggcgcaa tcgcccggtg ttaagaaact gcacttattg
    41821 gatgctgtac ttaccgaaac ttccgggtta atatatacca acaacatgtt gttgtcgcat
    41881 accgattcgg gaggggaacc gtgtctttac gaaatcaaca ttcatgatgg ccatattctg
    41941 aatacctatt gttataatac aattcataac tacgactggg aggatatcgc cacagacgga
    42001 aacgctatat atatcggaga ttttgggaat aactcaggct ccagaaatcc cttaaaaatt
    42061 tacagaatcg cattagatga tataggcaat cctgaggcta ccatcgattc aataagcttt
    42121 cgatttgccg atcaggaaga ctacacgcca atgtttcgca aaaccgacta cgattgcgag
    42181 gctctatttg ctgcaggtgg taggctttat gtgtttacaa aaaactggaa caacaaaaaa
    42241 accaaaatgt attccatccc cgccaacccc ggagactata gtgcagagcc aatacttgaa
    42301 cttgatgtga acgggttaat cactggtgcg gcgttcaata gcaatacaca ggttttagct
    42361 ctgattgctt acgaaaaaac ctttcctttc aatcctttga tgtttcttat tcgtttcagc
    42421 ggaaatttcg atcacattat caactacagc agaattaccc ttcaaattaa caatatgcag
    42481 acagaaggaa ttacctgggc cgacgaccac acactactga ttaccaatga gagtttttta
    42541 aaaacaaagt cgcacctgat gaaatcagat ttaaacacct gcactttact gccatttata
    42601 aaagacagct tgccagaccc tgaaaaactg ttggaaacag gatacaattt caacctgacc
    42661 atttctgata aaaatggtaa caaggttttt caggggaagc tacgaaaatt ctaccgcaat
    42721 caggaccaat ggcttggtgt gaagaagccg gcaagaattg agtggcatgc ccaaaaagca
    42781 agtggctggt ttatgtggca ataatcaatt cctcacaata ataacaatca catcagttcc
    42841 gagacattgt cagcatttcc tgaaaatatt tacacaaacg atacccgtat tcagcaaata
    42901 gcaggtatta cagaaaccgg acagggcaat catatatcat tatgcggatt ggcgggctcg
    42961 gcatccgcct attgtcttgc agcacttttc aaaaaaaccg gaagccgaat atgcattatc
    43021 ctcgacgaca aagaaaaagc agcctatttt tacgatgaca tggtgctgct ttcgggcaag
    43081 gataatgtgt tgttttaccc ttcttcctat aagcgctcgt tagagtacga aaaaaccgac
    43141 agcacaaaca tcgttctcaa aaccgaggca cttgagcgct tgagcaacag tagtaagccc
    43201 tatatcgttg tgagctatcc tgcagctata tccgaaaaag tgctaaaaaa atcagatctt
    43261 aaaaaaaaca caatgcacat tggttctaat gaccagcttt caatggattt cgttattgaa
    43321 ttgttgtttg aattcggttt tacaaaagaa gattttgtgt atgaacccgg atgctttgca
    43381 gtaagaggta gcattatcga tgtgttttcg tatggcagtg aattcgcttt ccgaatcgat
    43441 tttttcggag atacagtgga aagcattcgt gcattcgata ccggcactca attgtcgacg
    43501 caaaagctac gcagtattaa tattgttccg aatattcatg acttcgggca ggaacaggag
    43561 cgtatcagtt ttttcgaata cctgggcaca aaaaacctaa tatgtttcga gaacaaaaat
    43621 gcctttctga atcagattga aaaagttttt gatgcagcca tacttaaatc gaaaaccgat
    43681 aaaggtttat cggatgaatt agcctatcct gagcgatttc ttgttgcaca taatgagatg
    43741 accagattgc ttacggaatt caatactata gaattcagaa atgtccgcga tgaatcggta
    43801 agtttatcag ttgaattcaa cacctcggca caacccgttt ttggtaaaga attcgatctg
    43861 ctcggcgaag atattatccg acgaaaagaa gctgggtaca ccgtaaatat tctctcggaa
    43921 aaccccaagc aacaccagcg actcagagaa atattcgaag taagatgggg cgatatcggg
    43981 ttcgaaaccc cacagtttac ggtgcatgaa ggttttatcg accatgatca caaagtctgt
    44041 atctacaccg accatcagat ttttgagcgc tatcaccgtt ttaaggttaa agattcgttc
    44101 tcgcgaaagg atgcaatttc atataaagag cttcaatcga tcaacccggg cgactttgtg
    44161 gtgcatatcg acagtgggat tggcagattc ggcggattga caaccattga aaataacggc
    44221 aagctgcagg aagtagtgac aatacattat gccgataatg atgtgttgtt tgttaacatc
    44281 cattcgctgc atcgcatttc aaaatatcgg tcgaaagacg atacgtcacc ttcgattcat
    44341 cgcctagggt cgggtcactg gaaacgactc aaggaaaaaa ccaaacagca gcttaaagac
    44401 attgccagag aattgattgt attatatgcc aaacggtcgg agcagcatgg ctttgctttc
    44461 tctgccgaca attatctgca gaacgaactg gagtcttcgt ttatttacga agatactccc
    44521 gaccagataa aagttacccg tgaagtaaaa aacgatatgg aaaaaaccat gcccatggac
    44581 aggcttgtgt gcggcgatgt gggctttggg aaaacagaaa ttgcaataag ggcagctttt
    44641 aaagcagtgt gcgacggaaa gcaggtggca ttgcttgtcc ccacaacaat tctggctttg
    44701 cagcattacc agacattccg ggaaagactc gaaaaatttc cggttaccac atcgtatatc
    44761 agccgattgc ggagcccttc cgaaattaaa tcctcgctga aagacctaaa ggaagggaag
    44821 cttgatatac tcatcggtac acaccggctt gtggcttcgg atgttgagtt taaagacctt
    44881 gggcttctta ttatcgatga agagcaaaag ttcggtgtag ctgtaaaaga aaaacttaaa
    44941 aagttgaagc tcaatgtcga taccctgacc ctgacagcaa cacctattcc tcgcacactg
    45001 cagttttcgc tgatgggagc gcgcgatctg tcggtgctga gcacacctcc accaaaccgg
    45061 caaccgattg taacagaatt gcacacattc aatcacagca ttattaagga cgccatccaa
    45121 tacgaagtga accgcaacgg tcaggtgttt ttcgtaaaca accgtattga caatatttat
    45181 aatattcagg ctaccataaa aaaactatgc cccgaagtta gtgccattgt tgcgcacgga
    45241 caaatggaag gcaaacaact cgaaaaaatc attctcgatt ttatgcgggg cgatttcgat
    45301 gtactgattt ccacttccat aatcgaaaac ggcgttgaca ttcccaacgc aaatacaatc
    45361 atcatcaaca acgccaataa tttcgggtta agcgatctgc atcagatgcg cggacgcgtc
    45421 gggcgttcca accggaaagc gttctgctat atgcttgctc ctcccgaaga attgcttacc
    45481 cccgaagccc gcaggcggct gaaagccatt gaagaataca acgaactggg aagtggtttc
    45541 aacattgccc tgcaggatct cgacatacgt ggagctggcg atattttcgg ggcagagcaa
    45601 tccggtttta ttgccaatat tggattcgaa acctataacc gcatcatcag tgaggctctc
    45661 acagagctaa aagataccga gtttaatgac cagtttggca atcaggatcc agaaaaaaca
    45721 ggaattgccg atctgcatga atatgaagtt gccgactgca gtatagaaac tgattttgaa
    45781 gtactccttc catccgatta tatttcaagc acttcggagc gaataaatct ctaccgccag
    45841 ctcgatgaga taaaaaccga cgaagaactc gaagttttcc gtaataacct gattgatcgc
    45901 ttcggaaaac ttccacaaca ggcgattgag ctgctcgacg tggtaacact ccggagaatg
    45961 gccatgcaac tggggtttat taaaatcatc attaaaacca acctgatgat tgcatacttc
    46021 tcattcgacc agcaatcggc gtattattct caaaaaacct ttgcgtcaat acttcaatac
    46081 attcagaacc atcacaaaaa aatgttttta aaagaacaca acggaaaact cagcctgcgt
    46141 ttcgaaggaa tttccaacat acaggaagcg gtacaaagaa tacaaccatt agccgattgg
    46201 gtaaatcagg cgtagtgtgt tggttattta tcgtttttca aatcttcgta actgatcaac
    46261 attttcttgc gttgctccgg tttcggagtt tctgatttat catacaaagg tttttcccat
    46321 gtgccatagg ttgggtttgg aataattatg tatttcgttc cgaataagtc gatattattc
    46381 tcaaccgcat ctttgccaaa attctttgac cggtcgtcga actcattgct gaaatcgcac
    46441 aggttgtcgc ccacaaaaag caccactgaa tatttttcag aaattgaatc acgacgcatt
    46501 tttttatcgc tagtggtgct tttcaaaaga aaatttgcct tattgacttc cgggaaaccc
    46561 aatgatgcca tgttcttcag cgtccagtcc aattcacctg tcaaacgatt cgacacataa
    46621 aacacctcaa caccattatc taacgcaaat tttataaaat caacagctcc gggaagagca
    46681 gtggcttttt ccatttcact ccattcagcc caggcttcag gtgaaaaatc ctctcccctt
    46741 acactccgct ctgccaaaaa aggactatta tcgagtagtg tttcgtcaat atcgagtatt
    46801 accgctttgg gaagcttcga agtatcattc ttgatattgt tgagcaaggc tgattttgcc
    46861 acattatatg cctgataact gcaggctgtc atttctgctg aacgctgata ccatagcaga
    46921 gccatgtgca aatgctcccc tgtgtttttc gattcctgtt tgttttcctg aacaggttgc
    46981 acaggtttgt tgcaggcaat aataatgctg aagcctgcca atactaaaaa tgatgtaaat
    47041 ttcttcattg tgtgttggtt ttaataacac aaaactaatt attcgcggag attattgcaa
    47101 catggttttg atgtgtatat ttgctgaagc aaaataataa tcccatgaaa aacccccgcc
    47161 gactttttct tgtctttttg tttttataca ttggtttcaa tgcctatccg aatcccggcg
    47221 acacaacact ggtgcagtgt ttctctttcg acagtattta tgctcgccgg ggaatattcc
    47281 tttttccgcc gcagggaaca agctacgaaa aagtgctgat gtactatact atcaaatgcg
    47341 acccgctaac tccatgggat agttataact gcggagaatg ggactatact actttcacac
    47401 atatttatga acataccgga gattacgatt ccacactata cttccatccg tatttcacag
    47461 taaacggaat gcagccatac agcttttcat acctgaatag tccattagcc caaccttttc
    47521 cacgccgtga atatttctgg cagcatacca ataccatttc aatgaatttg gctgatattg
    47581 ggtccggatc gattcccgtg gaaactcctt tcggaaatgc agagaacgat gcaaaacacc
    47641 ttgtactttt caaagccgct gagctcacag aaggcggatt aaatgcaggt aatattaccg
    47701 gattgcggtt ttatgcaatg ccgacgggtg cggatgtgca acgtctgcgg gtacgtataa
    47761 agcctgttgc aatcgatgat atcggcgggt tcaattacct gtttaccgga ttttctacag
    47821 ttttcgatag aaatgtttcg attgacgaag aagggtggtt ctcaattgat tttctgaatt
    47881 actttgcatg ggacggagta tcaaatattc tggttgaatt ttcgtattca ggtataagcg
    47941 gatcgctgcc ccttgcagga gaagaactac tttacaactg ctgcctgact tccgaagcac
    48001 ccgacaatgc tataaggttc aatggccccg attatatcga agttcccgcg actagtgtgg
    48061 cagacctgaa ccagaaaata agcatcgcat tctggctgaa tggagatcat gccattcagc
    48121 ctcagaacga tatgctgctc gaagcctgcg atgcctcgaa aaaccgtaaa ctgtgtattc
    48181 atctgccatg gggcgatggt agtgtgtatt gggatgcggg caacaacgga gccggatacg
    48241 acagaattaa caaatctgct tcccccgacg aatataaagg caagtggaac ttctgggttt
    48301 ttactaaaga cgcttcaacc ggagtaatgc aagtgtatct gaatggagta ttatggttgt
    48361 cgggaacggg aaaaactgcc ttgctcaaca atataagcac tttcgtacta ggttcaaatg
    48421 ttgacaaaaa ccctgaatat tgttacgatg gtatgctgga tgaatttatg gtttttaata
    48481 cggcactcaa ccaaactgat attgaaaaca taatgtatca tcccttagaa gaaaccagtg
    48541 ctctgtggaa taacctgatt ttgtactatc cgttcgacga aggatcggga agtctgctta
    48601 ccgatgcctc tcaatatcaa cacaatacca atatgatggg tatgcctgac tggatcagtt
    48661 accatgggat caaccgatac aagtatttta ccgccggcat gctgcgcccc aataccattt
    48721 tcgaacaagc cgagtatgaa tcaacacttg attccctaac ggtatttgac acattgtatt
    48781 cacctcaggt tcaggttatt acctatgcca atcaggataa ttacaccata gttgccaccg
    48841 atacaagttt gtattacccg gccagattcc tcactgcgta cgatgtcgac ggcgttgccc
    48901 tcgattctgt gtacttctcc gacgctacta tttataatgc gtcgctcgaa tattactctc
    48961 agcctttcga agtgataaat acctatcagg tacaaaattt tgtgactcct tacgggattg
    49021 gtttaaacct tggctcgact ggttttacgt gggtttacga tgttaccgac tacatttcgt
    49081 tattgcacga ttcggttgac atcagtgcac acaatacaca ggaattgctt gatttaaagt
    49141 ttgctctgat cgaaggcact ccatcgagaa atgttatcga attcagtcag ttgtggcggg
    49201 gtaactacag tcatcatggc attgcctccg acgagcaact cccgggagta aaaatactcg
    49261 caaaccccaa tgcttcgatg ttcaccatca ggacaagaac cacagggcac ggaatggaag
    49321 gtgacggaaa ctgctcggaa ttctgcccga cctaccataa actctatgtt gacggagcac
    49381 aacgttacga atggcttaac tggaaagaat gttcaacaaa tccaatttat ccacagggag
    49441 gtacgtggat attcgatagg gctggatggt gcccgggtaa ttttgccgac acatacgact
    49501 gggatatcac cgattgggta actccgggcg atagcgtact gatcgactat ggaatggaac
    49561 agtaccccgg aagcaacggg gagggcaatt atcagatagc tgttcaggca tttcagtact
    49621 ccgctcccaa ttttctgaac gatgccgcca ttgatgtgat tgtagcacca acagatgctg
    49681 acgaattcgt gcggtacaat ccggtgtgca gtaatcccgt aatccggata aaaaacaatg
    49741 gagcaactac tctgaatagt ctggaaataa actatggcat ttcaggtaac acaacacacc
    49801 agtttatctg gactggaaat cttgacttcc ttgaaactga ggaagtaata cttcctgcac
    49861 tcagctggct cgattttacc ggagatagtc agagttttga ggtaacagta accaatccta
    49921 ataatacgga agatgaatat gagccgaaca atcatagtgt gtcacatttt gttctggctg
    49981 atgtttacga tcaggtaatt atgctggtgg taaaaacaaa caattatggc agccagacat
    50041 attacgacgt taccgacagt gaggggaata ttcttgtttc gcgcgacaat ctcgacaaca
    50101 atacctatta ctacgatact cttgattttg acccgggctg ctatgttatt cacatttacg
    50161 acagagacga gggttttatc gggcaagatg gactgaattt ctggtattgg gcaaatacgc
    50221 cctacgatga tggaaccggt tttgcaaaac tccgcgttcc gggcggggct tatgtgaagc
    50281 atttcaatgc tgatttcgga agctttttaa cctatcagtt tatttatccc gatgccagtt
    50341 atatactcaa cgaaaaagca aagaaagatt atttttcgct gtatccgaac ccgtgcagcg
    50401 aactagtaaa tgttgaattt tatgaagttc ctggaaccag cgattatatc tcgatttttg
    50461 atgtatcggg ccgtcaggtg aaacaaatac atcatatgag caacgacatt tgcattgatg
    50521 ttagcgattt acaaaagggg atgtatgtgt tgaaatatat ttctggaaat atcacttcaa
    50581 cccgtagcct gatagtgcag taaacaaatg actacaaagc tgcgagcagc aaatcgatat
    50641 ccctcgaaaa taatcctatt ttttttccaa tcgattcgtt ggtaagtatt ccattgaaaa
    50701 tatatactcc gttgcgcatt ccaggatagg atttcagaaa ccggttaatg cttcccgatg
    50761 tgtgaaatcc gagcagtttt ggtgccagaa tattgcttag tgcataggtg gcagttcttg
    50821 ccacgcgcga agggatattg ggaacacaat aatgcagcac tccgtgtttt ttaaagaccg
    50881 gggttttatg attcgtaagc ttgctggttt cgaagcaccc cccctgatcg atgctgatat
    50941 cgataatgac cgagtttttc ttcattttca taaccatttc ttcggtaaca agcatcatat
    51001 tcgtatcgtt ggtaatacgc attgcaccaa tggcaacatc tgctgttttc agtgcattct
    51061 caagtaccga aggttgtatg gttgaagtaa atacctgtat tcccaattgc tgttgcaagc
    51121 ggtcgagcct atacaccgaa tgatcgaaca ctttaacgtg ggccccaaga cccagggcag
    51181 tgctggcagc ataggttcct gcagttcctg ctccgagtat cactacttcc gaaggagaaa
    51241 caccggtaat tccacccagc atctcgcctt tacccttgtg cacattgctc aggtattccg
    51301 atgcaattaa aatagacgat ctgcctgcaa tttcgctcat agagcgcaca atcggaaaac
    51361 gggcataccg gtcctggatc atttcgaatg caatggcagt aagctttttt tgcatcagaa
    51421 tatgaaaata ctcatcgggc tgagtgctgg catgcagtga tgtaacaatc atctgattac
    51481 ctttaagcat tttacattct tctacggtaa aagggccaac cttaaggaca atatcgcatt
    51541 taaaaatagc ttctttctca tttgttatca aggcaccatg atcagcatag ctggtgtcgg
    51601 aaaaatttgc atttctgccg gattcgttct caatgatgat atcaaaacca tgggctgaaa
    51661 gctgttccac cgctaaaggg gctaaaggaa cgcgccattc cagaccagaa tcatcgcagg
    51721 gaattccgat ggtaaatcgg cgttctgtat tttgcacctc tagcacttct tcctgaggca
    51781 tcaggttata atgagtgagg atttggtgag gctggttttg tgatgacata gcgcttgggt
    51841 ttgtaaaaac ataaagttac aacataattt tcaaattttc ggattaaaat ttattgataa
    51901 aacgtaaacc tgaaagaaaa aattgtcaaa aaaatacagt aaagcacagg tatactaccg
    51961 cagcagctcc atgtcgattt gtttcttcga tagattaaca cctgtgatag ctactttcac
    52021 cgtgtcaccc agtgtaaact ttcgccctgt ggttttcccg ataatacaga aattatcctc
    52081 atcgaaaaag tagaaatcat cgatgagggt tcgtatgtga accaacccct cgcatttatt
    52141 ctcgaccagc tccacaaaaa agccccagtc ggtaacacct gagatcaccc cttcgaaagg
    52201 aaggccaatc ttatcgctga gatattcggc ttgtttatat tttaccgatg ctctttcggc
    52261 ctgcactgcg cgctgttcca tatcagaact gtgcttacac agccatttat agttatcctg
    52321 tacgcctgtt ctgccttgtg ttaaatggtt ttcgagtaag cggtgtacca tcatgtcggg
    52381 gtaacggcgt atgggcgaag taaagtgggt atagtgggta aatgcaagcc cgtaatgacc
    52441 gatgttttcg gtggagtatt cagcctttgc catagaccga atggcaaggc tctgaataac
    52501 attttgttcg ggtttgccct gaactttttc gattagtccg ttgattgatt tacccaggtt
    52561 tactgcattt tttgtctcta tcgaatatcc aaacttttta ataaaacccg aaaagctttc
    52621 cattttttct ggattgggtt catcgtgaat acgatatacc gagaccttac ccgttttact
    52681 cagcagttcg gcaactttac ggttggcaag cagcataaat tcttcgataa gccagttgct
    52741 ttctttgggc tccttaaaat atacacccaa gggcttgcct ttttcatcga gattaaactt
    52801 cacttcagtc gattcgaaat tgaaggctcc tttgcgaaaa cgctgctccc tcaataagtg
    52861 agcgaggctg ttcactgctg caatctcttt acttaaatcg ccttgtccgg tgtcaataat
    52921 tttctgcact tcttcgtaat taaagcgcct attggagttt atcactgttt tgccatacca
    52981 ttcatcaagt attttcgccg tgctgtcaag aataaaaacc gccgaaaaac acagtttatc
    53041 gacgtgtggc tgcagcgaac agatttcgtt cgacaaccgt tcgggaagca taggaaccac
    53101 cctgtcgacc aaatacactg aagtagcacg ctcctgcgct tcagtgtcaa taacagtagc
    53161 cggattaacg taatgggtga catcggcaat atgtacgcct atttccacac ttccgttgtc
    53221 gagcactttg tacgaaatgg catcgtcaaa atctttggca tcttcagggt cgatcgtgaa
    53281 ggtgagggtt tctctgaaat cgcgccgctt ctttatctct gaaaccggta tgctgccatc
    53341 aattttttcc gcttccctga tcatttcgtt gctgaattcg tagggtaacc caaactcaac
    53401 catgatggca ttgatctcag cctcgttatc accgggtttt cctagtacct tcactaccgt
    53461 tccggttgga ttcttacttt cagggtgcca ttctgtaata ctaacgataa ctttctcgcc
    53521 cgatttggca ccgttcaggt tgcccagagg gatgtagatg tcgtaaggta tccgtaaatc
    53581 gtcgggcata aagaatgcat attttttatt ccgttcaata atgccagcaa actgagtacg
    53641 tgctctctcg atgatctgta tcacttcgcc ctccacctgc ttgcttttac gcgatgcata
    53701 catctgtatt ttcactgtgt cgccgtgcag ggcattgtgt aaattccgcg gagaaataaa
    53761 caccggctcc ggggcttctt ccgaaagcac ataggcgtaa ccacgcctcg atacgtcaac
    53821 cttacctgta acataagttt tgggcatgat gtatttatat ttcccttcgt agacttcggt
    53881 gatgttcttc gactttagca attcggtcag aacttttttc acctcttctt tctcaagcag
    53941 atctcggacc ttcagccgtg ctgccatttg tttgtagttg aacaactttt gcggacttcc
    54001 ggcaaacaca ttcagcacct gctgcaataa tattttagcg gagtgttggt ttggtatgtt
    54061 gtttttatta aatttgctac tcatattgtt tggcattaaa attgtaaaaa taaaagctgt
    54121 ctaagataaa ctataatatt acacacttta ataataaaaa ccaaaacgaa aacaactact
    54181 atgaaaacga ctactatgat taaaactttg gctgttgtag caataattgg ctcaacagtt
    54241 gcattttcgg gatgcaaagg caaaaaagaa gcttccaatc cgggaaaagg ggcaaaagaa
    54301 gtggtaatcc cctgcaaaaa cgaaggcaga tcggataaag aatatttccg tgccgacaat
    54361 tcatcaacat cttccgacat gagcctgtcg cgtgaaaaag cccttacttt ggcaaagcaa
    54421 cgtctggcaa gcctcatcaa caccaagatc aaatcggtta ccgaccggta tgtgaatgaa
    54481 acagaggttg gtaaaaattc cgatttcgag cagaaatttg aaaatcttac tcgtgaagtg
    54541 gtaaaccaaa cgctttcgga tgttgctatt gtatgcgaaa aaactttcca ggaagccgat
    54601 ggcaaataca caacctatat tgcaattgaa gtaaacaaag atgcgctgct gaacggtgta
    54661 agcaataaag tttcgaaaga cgacaaactc cgtgttgatt acgataaaat gaaatacgaa
    54721 aaaatcttca acgaagaaat ggaaaaactt gagcaagaaa atcaataact gaaataatgt
    54781 tttttgaaaa gccggtaata attcaccggc ttttttatta tgtttatttg caataagatt
    54841 gcctttacat tattatcacc aatagtttaa taaggtatat attaatttct acttttgcac
    54901 aatgcaaatc attctcagtc agataagtat gctcggattg ctcgccgtaa taggtgcagt
    54961 tgctcttaaa acgcggttac tgcaagagga aactatgaat ggcattgtca agctcattac
    55021 caagctcacc ctgcctttgc tgattttttc gacctttgca ggaaacaaca tcaacggtga
    55081 aattctgaaa aacagttctg tgatcatcat tgtgagcatg ctcagcattg tgatgtttta
    55141 cctttttgca ggaatgagtg cccgcattct gcatctgaat gtcgaaaata cggcactaca
    55201 caaagccagc gccatgtttg gcaacgttgt attcttagga ttccccgtga ttaatgctat
    55261 attccctggc ggtgaaggac taatatatgc caccatgtac cagctcggtc aaaacacgct
    55321 gatatggacc tggggggttt acattcttaa caaaggtgct gataaaacct ctaaggaaaa
    55381 ctggaaacat cttatcaacc cggtaactat tgcttttgct gcaggcttgc tatttatggc
    55441 tatgccattc cggattcctg aaattattct caaacccata tccgacatag gacacacaac
    55501 gagttatatt tcgatgattt atgtaggtgc ggtactggtg aaggccaatc cattgcgggt
    55561 ttttgtaaat ctgaggtcgt atctggttag cgtaaacaaa ctggttctta ccccactgat
    55621 tatactgcta ttgttcaaat atgcagggtt aagccagttg ttccatgtta gcacaaccgc
    55681 tctgggagta atggtaatgc tctcgggaat gccttgcatg atactgatat ctgtgcttgc
    55741 aaaagacctc gggttgaacg acaggcagtc ggtggaaaat attttcctaa gtaccatact
    55801 ttcgtttggt acactgagtt tgttgttctg gctgctttag tgtatggtaa aattcaccag
    55861 atcgttgtaa gttcctacaa acaggtcaat atcaacctta ccttttatcc cggcaacctt
    55921 gccttcatgc gaaaactgcc agaacttcca ttgcgctgca atatgcgagg gctctttcga
    55981 tacgtttaat gacgatatcc ataccggata tttttcgaac cgtttatcac cattgaacat
    56041 actgcggtag atattcggag atgtgtaaat aataggtgtc tgctgataat gtttttctac
    56101 tatttcgagc cagttgagca gtccggtttt gaggctgttt ttgttttgca catggctgta
    56161 ttgttctata tcgagcacag gaggcaaatc gcctttctgc agagtaacag tttcgatgaa
    56221 ccgttgtgcc tgaccggtag agttttcgtt gggacgataa taatgatatg cccccctgat
    56281 tagtttttgt tcgccggcag cataccagtt ggtgataaaa tttctgtcgg ttttgctatt
    56341 tcctgcagta gccctgatga agacaaaata catacttttt tcggaggcca ctttcttcca
    56401 gtcgatactt gcctgatact cagatacatc tatgccaaat accgtgtagt ctttaaaatg
    56461 cattacatac gattgaaaag atgtatcgcc ggtatagcgc tcctttttca ctcttgtgtt
    56521 gtctcccgaa aaaaacacca gatatactgt tgctgcagca attaataaca gaagaaaaat
    56581 aaaaaccccg accagaaaat tgttcttttt cttatttctt ctgatctttc gtcggggcat
    56641 attgcaatgt tatttaataa gatattcagc aatctgaaca gcgttggtag cagccccttt
    56701 gcgcaggtta tcggctacga tccacagatt gatggcgtat tcgtgcgact cgtctttccg
    56761 gattcggccc acaaaaactt catcccgatc ttctgcccat ataggcatgg gataaatatt
    56821 ggcagtgggg atatctttca ccacaatacc ctgtgtttcg gaaagtgtac ggcgaatttg
    56881 gttgatgtcg ggtttttctt gaaattcgat atttacactt tcggaatgtc ctccgattac
    56941 cggtacgcgc acaactgttc ccgttaaggc aagttgcgga aggctgagaa ttttccggct
    57001 ttcgtttacg agtttcattt cttcatcggt gtatgaattt gccagaaaat tgccaccgtg
    57061 ggggatgcaa ttcaggtcga tctgatgcgg gtaagcatca tgtaaagtcg atccggctcg
    57121 ctcaccgagc aattgtttta cccctttgta acccgaaccc gaaaccgact gatacgttga
    57181 cactattacc cgtttaagtc cgtaaagttt atgcagcgga tacaacactg ccacgagctg
    57241 aatggtggaa cagttgggat tggcaataat ttgaggtttt ttatccagaa gcgttgcatt
    57301 tacttccggc acaacaaggc gaatgtcatc atgcatcctc catgccgacg aattatcaat
    57361 caccgtacag cctttttcca caaaacgtgg agcccactcg agcgacacat ccttccctgc
    57421 cgaaaacagg gctatatgtg gttgcatggc aaggccttcc tgcaaacagc aaacaggata
    57481 ttctttaccg ccaaatagga tattctttcc tgctgaacgt tccgaagcca caggtattag
    57541 tgtagtaacc gggaaattat gttctgcaag aactttcagc attttctgcc ctaccaagcc
    57601 tgttgcacca actactgcaa ttttcatgtg ttattatcat taataaatca gacatataat
    57661 cgcttttgca aaattatgag atccttgcaa aattcaatca ttccatatca attcaacccg
    57721 acagaatatg attccataaa cggattaata accaaacaac tgttccagtg ttaattcttc
    57781 gactttacca tattttttca gcagggtaaa attgatctta tctttcggac ctactaccaa
    57841 aatatcgtat tttttgtctt taatatgtcc gtcgaaaaag ctttttacat cattcaatgt
    57901 aaatttctga atatcggcat acatttcctt acggtaatcg tgatcgatac ccagtttctg
    57961 taacgcttgc caattccagt aaatatccga tttaataatc cgttcgctat tgatttgctt
    58021 aatgatggca tctttgctgg tgccaaacaa ttgttccgac attgccaatt cgttcagcaa
    58081 tccggtaaga gcatcgagag cctcggtcat tttatcgggc tgggtactca ggtagccgaa
    58141 aacataattc gatcttcctt tttcgcttgg agtgcggtag ccggcatagc ttgaataagc
    58201 aagccctttg gattccctga tctcctgaaa tactattgaa gacatgcttc caccatagta
    58261 ttcgttgaac attctgataa taggtatctg aagcgggtcg tattttgtat ccttgctaac
    58321 gagggcaatg agggtttgaa ccatatcata atttacaaag tacaccgtcg gggcggtata
    58381 gtctttttcg ggatacacct tgcggggcgg agtttcctta agagtagcgt ctgttttatg
    58441 gtatttctga atgagctcgg ccattttact ttgttcgcgg ggtccatagt aaaatatttt
    58501 atgtttatac ccgagcatct gatgaatcag gtcaaccagt tgttgcggat ccattgccct
    58561 gaggtctgct tccggaataa gatcggtagt cgggttcagt tgtccgtatt tcgaaaagtt
    58621 catcatccgg ctctgaagga tagtattctt attgagcttg gcatcgttgc gtttttttat
    58681 gatatcatca acatagtttg cataggcttc tttatcaacc tttgcgcctg caagaatttt
    58741 ctccagcaat tcgatgcctt tttcgaggtt ctcgtcaaga ccgctaagat atacatacga
    58801 ctgatcgtcg ccggttgaaa caccaaagct tagcccgagt ttgtaccatt cttttttcag
    58861 atcttcggca gttttatcgg aagtaccgag atactcgaga taattaatcg caacaggcaa
    58921 ccatgcatcg ttttctttac ctatatcaac tatataatac aagtcgaaca actcgttggt
    58981 ttcgtttttt atatagctaa aatcgatggc tgtgccaact ttttgtgttt ttatcaggtt
    59041 tttataatca acaaacgaag gttctatgtc ttttgtattc atctttttga ggtcggtaac
    59101 atattccgac tcagtagtac ggttgatatt aagtgccgta atgggtggtt tttctacgtg
    59161 atatgtatta tcgttggcac cattgcgttt atacacaacc acataattat cggcgaaaaa
    59221 ggtttttgcg aaatcaacga tttcctgttt ggttaccttg gcaagcctgt cgttatattt
    59281 aagaatatcc tcacggctgc gtcccgaaat aaactgatcg acaaattcat acaccgcgta
    59341 gttactttcg tatgcctgta tctgactgag tttgcgctgg ttgataattg cttcaaggag
    59401 ccattcatcg aagttgcctt ttttaatgtt ttcgatttca ccgagtaaca gcgtcttcag
    59461 ttcttcgagt ttctgtccct gacgcggact ggcactcagg ttaaaaacgc cgtagtcgtt
    59521 catattgttc acataggctc cagctctgag cactttctgc ttctgtacca gattcagatc
    59581 tataagtcca gcttcaccat tgcttagaat ttctgcaatc agagtcagaa ataaatcctg
    59641 atcgttatgc aaaccttcgg ttctgaacga aatgctcatc gattcctgat cgggaccagt
    59701 aacagagaat tccttaacag cggtaatcgg gtcttcttta ggagaagtaa attgaggtac
    59761 attttcattg ggtttccaat cgccccaata tgtatcgata gtttttatca tttcttcggg
    59821 atcgaaatca ccactcaggc aaatagcaat attattggga acataataat cggctttgaa
    59881 tttcatgata ttcaccatgg atgggttttt cagatgctct ccgataccaa ttacaggaac
    59941 tccgtagggg tgttttttaa ataattccga catcacttta tagtaagatt tacgataatc
    60001 gttatcctgg ctcatattaa actcttcgaa aaccgtttcg agctcggtgt gaaataaacg
    60061 cagcaccaag tctctgaagc gttctgattc cattttcacc cagcgttccg attcgttcga
    60121 aggtatgttg ttcacatata cggtttcttc gtacgacgtc catgcattgg ttcccgtagc
    60181 acccatcatc gtacagattt tatcgtattc attggcgatg gcatatttcg atgcttcctg
    60241 cgacagactg tcgatttgcc tgtagatggc cagtttcttt tccggattgg tttcggcagc
    60301 atgtttttcg aacagatcgg atatctgttg tatcagtgct gattcttttc cccagtcgag
    60361 tgttccaatt tcatcggtac ccttgaacat catgtgttca aaatagtgag ccagccctgt
    60421 agtggcacgg ggatcgttct ttgctcctgc tttaacggca atcgccgtct gtatgcgggg
    60481 ctcatcctta ttcaccgcca tatatacttt taatccgttg ccaagagtgt atattctcgc
    60541 atttaccgga tcgttggtta catattcgta agtgtaaccg ttggcatctt ttgcagtttt
    60601 ggttttatac cctttgttgt tgcatgatgt aaaaagcact aatggaatca gtgcaataat
    60661 ccaaagtttt ttcataaatg atttttttaa atttgggtta aatttacaaa ttgttttaat
    60721 cggttttaat atgaaaaatt atatcagtga aaaacaattc ataaaatttt attaacgtaa
    60781 gtcatatcaa tgcatttgta acagtgaaat agcatgaaaa ctagtaacat cattaccatt
    60841 atggcaataa tacctttgtg catactatcg tgccatcaac ccgaaaagaa aactgcgacc
    60901 aaagaggttg aacctttcaa ctatgttgcc gaacaatttg ccgatattcg gattctgcgg
    60961 tatcaggttc ccgggtttga aaaactcggt attaaggaaa aaaagtttct ctactgtctg
    61021 caggaagcag catactgggg tcgggaaatt ttttacgatc agaactataa acacaacttg
    61081 tgtataaaac gcaccctcga agcaattgtc gaaaattatc agggcgataa gaattcagca
    61141 acatgggtaa ggtttatgga atacaccaaa cgattctggt tttcgaacgg aatacaccac
    61201 cactacagca acatgaaatt tcttcccggt ttcagcgaag atttcttcag ggaacaggtg
    61261 ctttctgtta aacctgaatt gttgcccctt tccgagggag aaacccccga aatgcttgtg
    61321 gaaaaactta tcccaatatt gtttagccca tcggttgctc cggtaaggtc ggagtcggac
    61381 acacgcaaag acctgattgc ctcctcagcg acaaactatt acgaaggcct ctcgcaacaa
    61441 gaagttgaaa ggtattataa aacacgaact aataaaaagg acagtactcc catttcgtat
    61501 ggtctcaact caaaaatgat taaaaagaat ggagccatca ccgagcaagt gtataaacac
    61561 gaagggatgt acggcgctgc tatcgaaaaa attatgtatt ggctgaccga agcaaaaaaa
    61621 tactgcgaca atgcagaaca aaccgttgta attgataaac tcatagaatt ctaccggaca
    61681 ggcagcctcg aaacttttga tgagtactca atactatggg tgaaaaacag taatgtcaag
    61741 gtcgatttta ccaacgggtt tattgaggta tatgccgatc cgctggggta tcgcggggca
    61801 tggcaaagtg tggtgtatgt tctcgacgaa gaccttacac ggaaatttgc caacatttcg
    61861 gcaaaagccc agtggttcga agacaattcg cccattatgc ccgaacacaa acgcagcgaa
    61921 gtggtaggcg tcagctataa agtaattaat gccgttgccg aagccggcga ttgcagtccc
    61981 tcaacgccca ttggagtgaa tctgccgaac tccaactgga tacgttcgca atacggttcc
    62041 aaatcggtat cgctgggaaa catcgaaacc gcctacgatg aagcctcgaa aacatcgggc
    62101 gttctcgatg agttttattt gcccgatcaa aagaaaagaa ttgaagactt tggtgctctt
    62161 tcagggaaaa tacacactgc cttgcacgag gttattggtc atggtagcgg aaaaatcatg
    62221 ccaggcgtag gcacacccaa agaaaccctg aaaagctaca gttctgccct tgaagaaaca
    62281 cgtgccgacc tggtagccct gtattatatt atggatccgg ttgtgattga ccttggtctg
    62341 atggatacca ttgccaccgc atacgccgaa tacgataatt atattataaa cgggatgctg
    62401 aagcaactgg tgcggattga gccgggtcag caaatcgtgc aggcacacat gcgatgccgt
    62461 tcgctaatca gccattggtg ctacgaaaag gggaaaggtg aaaacgtgat cgaaaaagtg
    62521 tatgaaaacg ggaagaccta ttataaaata aacaactatg cccggttgcg cgaactcttc
    62581 ggagaactgc tacgcgaagt gcaacgcatt aaatcggagg gcgattacga agccggaatg
    62641 aaattggtcg aaacctatgg tgcgcccgtc gaccgcaagt tacacaagga agttctcgac
    62701 cggtatgcca agctcaacgt ggctccctac aacgggttta ttcaacccca gatggaggcg
    62761 gtttataaaa acggagaatg ggtggatgtg aacataagtt accccgacag cttcgagaaa
    62821 caaatgatgt tttatgcaaa ggaatataaa atccttccga cgtataatta ggttttatct
    62881 taattactag ctaaaattat ctattaaatg atttaacctt tctctaataa ttcaaacttc
    62941 aacattacta gtgatgcgtt aagtttaaaa aatgttttat aataatttaa atatcaataa
//
LOCUS       DAOOQP010000004        61530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_525_length_61530_cov_6.922651, whole genome shotgun sequence.
ACCESSION   DAOOQP010000004 DAOOQP010000000
VERSION     DAOOQP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 61530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_61530_cov_6.922651"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="PLK75_00780"
     CDS             complement(<1..2313)
                     /locus_tag="PLK75_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY37809.1"
                     /translation="MQLINNTEGSIFQKLFYKHPAPAIWGHWVLCGDKKLYQIDDTNE
                     SFETLSGISRELLHGLFINRKNKSISPKAEKIIQALQIAVENRTGILKPGYYCSEEKC
                     YRIDFTSIDENFFMYSFSDISVEKEQINESESFFELIPDLLSVIGDSQQLLKINSAWE
                     RLLGYKKSEILERKFTDNLHPDDVNEWHASLNSIKQHTGISSFTNRIFCKNGEYRFFE
                     WRVFAYGNKLFTAARDITNRIEKNTRLEHINSFSNDLLQLYSENENIDALLRKLIEYS
                     GATFCALNQFNESTKEYSTVSVIGDQGVIKAVENILGFRILGRRWQYNPLSDTPFNKK
                     ESFSYKSFLEIPRFIVPGAISRKLQCAIPVGNIAAIRIKNQNSLLADIIFFTPRGVEI
                     KLFTEYEVYLRQITLFLKGISTEQAFRRSEAQLRQIIDMVPHFIFAKDIYGKFILANK
                     AVASAYGTTPEDLIGKTDSDFVKDKKQVNHFRSDDLKVIKSGKELFIKEEVLRTENQP
                     DKVLQTIKIPFTNYNDTDKAVLGVSTDITERVLAEKKVLNSEARLRTIIEMAADTILL
                     GSADGRIVGANDQAEMLTGYTKEEITKMRIEDLFAVEELSRVPMRYDLLMEGKSVRSE
                     RLLKRKDAQLIPIEMNTRVMPDRSLHSILRDIEEIKRVQNELIAAKEKAEENDRLKSA
                     FLSNMSHEIRTPMNAIVGFSNLLTDPGTPNDKKTYYSEIIDNNCKSLLRIIDDVLDIS
                     KIESGQMKIVNASCNVNAVLEKLHEAFTIFI"
     gene            complement(2333..5062)
                     /locus_tag="PLK75_00785"
     CDS             complement(2333..5062)
                     /locus_tag="PLK75_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY37810.1"
                     /translation="MNAKPNTDDLLKRKEIFETTLQSIGDAIICTDTKGNISFMNRIA
                     EKLTGYMLEEVFEQPISDCFNIVNAFTREAAENPIEKVLTTGSVVGLANHTVLISRNG
                     TEYHIADSGAPIISDDKQIIGVVLVFRDVSKQYLAEEKLKQSEGRFRSLFENMKEGVA
                     IHDLVFDTKGAPSNYRIIKANPAYQVHTGISLPAGYNKLANEIYQTPDPPYLNEFSQV
                     ALTGKSCFFETYFAPLKKYFQISVVAIKPNSFATIFEDITPTRVMINELTESEERFRK
                     IFEESAEATLILSNNKFMECNKAALKLLKFKSKKSLLGKSPALISPVYQEDGLLSEDK
                     ATEMMNIAFTKGSHKFGWTHLCADGSTVECEIILTSIIYKGSTMLHTVIRDDSERKHA
                     MRRLSMSEEKYRTLYETMAQGVIYCDNSGEIISANLSAKRIFEEDNLEGRSITSGSLG
                     WTTSHLNGQPFKMEDYPVLKAFKTGRRVKNITMGISGGNLSRQKWLNINVIPNKDKIT
                     KNVKSVYVTLEDITDLIVYEKALLLEKQRAEESDKLKTAFLANMSHEIRTPMNAIMGF
                     SQLMLNSGMHESSRQYYSEIINNSCVSLLRIIDDILDISKIESGQLTIVNSTVDINNM
                     FDNIVSVFAEMISEKRGNAVRLIYKKPSQTVLVKTDETRLLQVLNNLIENAVKFTETG
                     YIKIGFHINESMLEFFVEDTGIGIDKKLHDKIFERFWQAEIARTGPPRGTGLGLSICR
                     KLVELMGGKIGVKSIKGKGSKFTFTIPFLQAVEVCVEQNKAGKINKQRRKLRLLVVED
                     DLINSQYLKEILKRYDNCESEFAFDGTEAINMALKNHFDVILLDIQLPGTNGYEVARI
                     IRKHNTEVIIIAQTAFAMPEDKQKCISSGCNSYISKPINFRDLFDMLDGYAYI"
     gene            complement(5640..6560)
                     /locus_tag="PLK75_00790"
     CDS             complement(5640..6560)
                     /locus_tag="PLK75_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOY37811.1"
                     /translation="MKNLSDIEHIREFDNLDLIAQQVVEGFITGLHKSPFHGFSVEFA
                     EHRLYNAGESTKHIDWKLFARSEKLFVKRYEEETNLRGQIIIDVSSSMLFPYKSKEAN
                     KLLYSVYCAASLIYLLRKQRDAVGLSLFSDEVIFHSEPRLSQVHVQVMYNQLRLLMNS
                     ENIPMKKTTNTATCLHQLAETMHKRSLVVIFSDMFGSNDPELLFPALQHLRYAKHEVI
                     LFHVTDHKLEQQFQFSNRPYKFVDLETGGNIKLNPNEIRDTYVKNVEEYVNELKLKCG
                     QYQIDFVEADINSDFSDVLTAYLLKRQKYF"
     gene            complement(6588..7001)
                     /locus_tag="PLK75_00795"
     CDS             complement(6588..7001)
                     /locus_tag="PLK75_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37812.1"
                     /translation="MKEFHVESKVVKVPQHDEKIFNFLSDFRNFNSFIPPDLEEWTSD
                     AESCSFKAKGQRISLIFAAKEPFKTIKITSKAENPFEFFFWIQLKAVDSSSTAIKLTI
                     EAELNVIMRNMLEKPAKQMLDKLADALCNLSYQTT"
     gene            complement(7014..7646)
                     /gene="pyrE"
                     /locus_tag="PLK75_00800"
     CDS             complement(7014..7646)
                     /gene="pyrE"
                     /locus_tag="PLK75_00800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOY37813.1"
                     /translation="MQNIAKQIAEQLLQIKAIKLDPTNHFTWASGWYSPIYCDNRKTL
                     SFPEVRTFIRDSFVKIIGMNFGDFDVVAGVATGAIAQGALVADALKKPFVYVRSKPKD
                     HGLENLIEGYLEKGKKVLVVEDLISTGGSSLGAVKAVRAENCEVVGMVAIFSYGFDVA
                     KTSFENENVKLVALSNYDVLIEHAIETGYIKAEQLKTLQEWRVNPSVWKK"
     gene            7653..8282
                     /locus_tag="PLK75_00805"
     CDS             7653..8282
                     /locus_tag="PLK75_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY37814.1"
                     /translation="MEKIYFKNRFISICESPVNRKGAMNINYQTVHDLNIIIYKFLAN
                     NHISSLHIFGKKPKKILKKIKTVFHYIKAAGGVVVNETDEILLIRRRGKWDLPKGKKE
                     KKEDIESTAIREVCEECGLNPELLKIENFIDNAFHIYEEGPDFILKRTSWFVMKYSGT
                     EVLIPQTEEDITKAKWVKPDELSRYLKKTFLNITVILEKYLKSTGVIID"
     gene            complement(8288..9688)
                     /locus_tag="PLK75_00810"
     CDS             complement(8288..9688)
                     /locus_tag="PLK75_00810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOY37815.1"
                     /translation="MMRNIAFLLFFSVFTTNLYAGICKVEGTAPAYAGKKIVFQKFYD
                     RITMLEQDLFTVTVDSKGYFSGQCELDKTCMVFAETGIYFAYFYMEPGCNYDIILPEY
                     IEKTKADELNPFFEPESVHIGIKNMKKTDLNYLIMDFDYFYDEYVDESMFEIYVKGME
                     SDVDTFINEIDSHFSYKGNPYFNTYKKFKYVGLRYLAYQRNRPEVAFKNLTFDSVYYE
                     NPAYMEIFGELYSNFFDNQLNELIGPRLFWEVNYGHSITNIKLLLSYWLELQNPRLKE
                     LAILKGIHDAFYNDNYAWTPLLLCLDSLCIVTAYPEHRVIGQNIADKVLTMAEGTVAP
                     HFALPDTLGDTVKLSNYRNQFVYLNFINTDSYTCQQQLELLKTIYEKHNQYFKVVSVV
                     VDSDVAKAKRYLNAQKYTWDFVFTGGDKTIPEYYKIVAYPSYFLVSPSGFLIYSPAPG
                     PLEGFEKAFFSLINDD"
     gene            9918..11522
                     /locus_tag="PLK75_00815"
     CDS             9918..11522
                     /locus_tag="PLK75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOY37816.1"
                     /translation="MGALSGGPEDKEPPVAVFADPPNASVNFAATQVKIRFNEYIILK
                     NVQQALIISPPIDPKPEVLVSGKNLIINIEPGALRENTTYNLNFGDAVADNNEGNPLK
                     GFTYSFSTGNLIDSLMVSGTLVDALTLKPIEGASVVLYKEGNDSLFFTTIPQYLSVTD
                     ANGKFSLQYLAEANYLIYAISDANYNYYFDQPSEPIAFTDAIIYPTVSLATVPGDTAK
                     QQPVYAPANVLLKMFTEDYQKQYIKSAERKEPGKVQIIFNMPNRTIPEISLPDDVEFL
                     ISTSATNDTLTLWLTYENMSLHDSLNIYCSYYDKTTDGVLVYDTIRITKPLKNENSLL
                     QFSGSRHKELYSDYLLNFSHPIVSFDSSKIVLQNVESPEKRIDFTIASAIKPEQMLLK
                     ANLAEGEEYVLHCDEGAFSDCYGGSTKSDSIRFTVKTEADYGSLIISIPTAPETCFAE
                     LLVQNKVKYSVRIADNRLAFMNISPGKYTIRIITDSNSNGVWDTGNLLLNRQPEAVYY
                     YNSEYEVRSKWQHELEWQQKFNEQGQ"
     gene            11519..12832
                     /locus_tag="PLK75_00820"
     CDS             11519..12832
                     /locus_tag="PLK75_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37817.1"
                     /translation="MKKVLIISYYWPPAGGIGVHRCLKFAKYLPLFGWEPVVYAPSNA
                     RYEYTDTTNFRHINADLTVIKAPIVEPFGAFRFLSGRKKAETTNPVYVRDRKRSLVDK
                     AGIWIRGNLFVPDARMLWIRPSVKRLKKYCRENQIDALLTDGPPHTNTVIALRLSQTL
                     GIPWLADFQDPWTQVDYYSMLYIGKCADRKYHRLEQESFATAKKITIASPTWARELES
                     IGAKQVDPIFWGYDDDDFPEALPPYSEYFSIVHAGQLGYDRFPATFLKVLADLCNENP
                     QFRDKMRLELAGTVDYSIAQRIGELGLNSNYKPLGKMSFPDAVKLTLSAHLLLLPLNI
                     ADNAKGRIPGKLFENIKARRPILCLGPSGSDSGNIIAETLSGESFEYDNEEGIRNYII
                     RIFEKYQNNCNVLPPYDITAYSVKNQVKRLATYLDQISNSNITKP"
     gene            12829..14274
                     /locus_tag="PLK75_00825"
     CDS             12829..14274
                     /locus_tag="PLK75_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOY37818.1"
                     /translation="MKSYRPAFAIVTVLFFMWGFITCMNDILIPFVKAVFELTRAQSM
                     LIQMAFFSAYFFGSLIYFIISTTTGDPIQKIGYKNGIVAGLIISAIGTGMLYPAAGLR
                     VYGLFLAALFILGLGFTLLQIAANPYVAILGEEKTASGRLNLSQGFNSFGTTIAPIIG
                     GYLVFHLFSGKGLSLLNAQGNPITLADGSTVSAMGVQIPYLIFTFLFVVLALVFIFIK
                     LPQFSDETHVERGLGALRYRHLLFGMFAIFFYVGGEVSIGSIMINYLHELKGFAEMDA
                     KSYLALYWGGLMIGRFIGAYAFGQQKISAITFLGMLGISILGFGVIYTAIYLEGGKSL
                     HLFLPYIAYLALNILAFYFGRTRPGLTLSIFAGIALLLIGTSMFTHTDLAMWSILGIG
                     LFNSIMWSNIFTLAIKGLGKYTSQGSSLLVMMILGGALLPFITGHVADILGGYQKALF
                     VPAISYLYLMWYGLKGSKQKNTIQDQSKYSA"
     gene            14352..14927
                     /locus_tag="PLK75_00830"
     CDS             14352..14927
                     /locus_tag="PLK75_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37819.1"
                     /translation="MLYLHYSDSVKQVCGDSVLSNYLVDEKLREYASNLDSVSFFIID
                     ENECFITSVKGIPLCTSCGIAANRVMQTAYRLAFIENFIIQGSCWDFVNAVFTGITSD
                     SIQKKDIYSEKKAGPYTTVDELQPGDWVYHVNYEYNRIEHSAIFIGWADYEKGLAIVL
                     DYVGLNRRQPAKFGEHNLNSVYAIFRMEKVD"
     gene            15009..16553
                     /locus_tag="PLK75_00835"
     CDS             15009..16553
                     /locus_tag="PLK75_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOY37820.1"
                     /translation="MDKIKSIKETNKADTSANGSLIPEFIPDSSLNFNFWAEVTGAGD
                     TVIADSLFKGFNMDYSDDTVSIFCFRGSCRRNAPSRGTVTGKPTGLELAWVFTTESDT
                     TKTKYGVWGGGAGWTGQPLLLCWTNEQKQKLGINDSQFLNNDNAFELIIGSLCGNIYF
                     LNASTGRPTREHLSIGSPIKGTVSVDPRKNGLLYVGQGIQTGEKFGAYVFDMFSGKEI
                     YHIPGNDTYSYRDWGAFDSNPLVDKESGTVFWPAENGVIYRFVADSLKQIKQLTRFHY
                     RHDRIFRQGIESSMAVIGNYGFLADNSGTLLCLNLLTMKPVWNIDNFDDTDASLLIDE
                     ESPGQYYLYTGHEIDKLAPVSASYFRKIDAKTGHEIWRISRECHGDDLNGQVNSGGML
                     SSPALGKHKGNNLVYCLYSRTDSLNRSELVAVNKTTGQEVFSVQLDNYSWSSPVDFYD
                     TEGNIYLFFADVKGKFYIIDGLTGELLVTKTTQFSFEASPVILNNKIYIASRGKSILC
                     FIIKSL"
     gene            16643..18310
                     /locus_tag="PLK75_00840"
     CDS             16643..18310
                     /locus_tag="PLK75_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37821.1"
                     /translation="MKNLLFAIFMSITLISQAQTPVAPAGTGTENDPYQITTLNNLYW
                     MSVTPTSWDKYFEQTDNIDATSTATWNSGAGWDPIGYVNGESDYLAFTGYYNGNNWLI
                     GDLYINRPTELAVGFFSIIENAHISHMGIYNASTDITGGEVTGMLAGFNLSSTIIDCF
                     GVGHIYGVSDLGGLIGRNETGTEYLYDCSFDGVVVGTGDNIGGLIGKNKGTVRNCNTF
                     ASITGTSNLGALIGENNGDFAVVDSCRAGGAIYVLGNEPTVNAGGLIGENKENAVVSY
                     CFSHTQIQGTENSENLGGLVGLNDNGSIIKYCFSTYFVSGGGSLGGLVGSNSYSTISN
                     CYSRSNVTGTQNNVGGVVGRNTGTVEKSYGTGVVTSSGGCFGGVAGSNEDGGEVFACF
                     WDTQTSGVAEGCGYNGGGMLDIVGKTTVEMKTQTTFTNAGWDFYGESANGTEDNWFIT
                     QYTNDSYPCFKWYTDFVGIRETSNDKNTFSVFPNPASDIIFVTVFSGETEITKLSIYT
                     ITGSLLYSAPHYQSNEPIDISNLKTGSYFIELKSAESKSTIKLIVNR"
     gene            18469..20328
                     /locus_tag="PLK75_00845"
     CDS             18469..20328
                     /locus_tag="PLK75_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOY37822.1"
                     /translation="MRFRGLAYLPAILFWWLICFQLYRLVFFVIITSCSGNFAEGIYH
                     GFVTDLAAAAYLMVLPGTLWFISNFIKTQNRGFFKIILITQIVLALFVAFIEVVSLPL
                     YPEWGTTFNLRALVSAESPGEAFTTFVDFLSWKWLLLFFAEAGVAITGFYFIQNYFYY
                     RLANKSVRIIYPIIIALLLVLGIRGGVGKKLITVGSVFYTHHQSDNYLAVNKTFYFIS
                     TVKQKKHLDIIKTDIQKEQLNATYRKLYIEGKASDTLRVFDTPRPNIVLIVLEGCPAE
                     VFSPLGGSPGIAPCFEKLSSEGLLFTQFYASGFRTDQGLLSILSGLPALPYLNIMTDP
                     KIAEALPSLMQVLGEKGYNTAFIYGGDASFSKISDYMKSNNTATIIDKASFNKHQCRI
                     EWGAPDDELFIMAGQQIKKYSEPFFACILTQSTHTPFEIEGQHKFPGENIPEKYKSSV
                     YFTDSCIGSFIERSRQTSWFNNTIFIITADHGSLYLGTHDFNDHERFRIPLLIFGTPL
                     AEQYRGKTISRVGASFDIPATILAQLNIENHDFPFSKNLFHKKMFDHAFWATDQIMGW
                     ITNDKKIVFNFSNSDIYSTEGDTVHNAESVEDAIRFYKLVAEYALLKKTPSVR"
     gene            20435..21379
                     /locus_tag="PLK75_00850"
     CDS             20435..21379
                     /locus_tag="PLK75_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOY37823.1"
                     /translation="MNDKNLIAGIDIGGTGTKIGIVDFDGKIIAQALIDTREFSTMEL
                     FVSACIKKISSLSNLHRLAGIGIGAPNGNYYSGCVENAPNLPWRGKLELAGEFTKQSG
                     LKAILTNDANAAALGEMMFGGAKGIGHFFYITLGTGVGSGIVADGKLIYGHDGMAGEL
                     GHTIAVKGGRMCGCGRKGCLEMYASVRGLVLTARELISQYPGQSALESKIDFSAKDIS
                     DAAKNGDAIALKTFDITAEILAISLANASAVTSPSHIFIFGGLAKSGELLMVPLKKYY
                     EQSLLNVYQNKVCLSVSELPDSDAAILGAAALIRNYFM"
     gene            21449..21841
                     /gene="rsfS"
                     /locus_tag="PLK75_00855"
     CDS             21449..21841
                     /gene="rsfS"
                     /locus_tag="PLK75_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOY37824.1"
                     /translation="MITTRLTEDETLLQSINMAILDKKGEDIINVDLRNTTGSVTNYF
                     VICNANSITQVQAIAEHVERKVRTELKEKAWHSEGYENCVWVLLDYVSVVVHIFEKKN
                     REFYKLENLWADAVFSHIGNDGKIALVP"
     gene            21885..23963
                     /gene="ftsH"
                     /locus_tag="PLK75_00860"
     CDS             21885..23963
                     /gene="ftsH"
                     /locus_tag="PLK75_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOY37825.1"
                     /translation="MSENRKGFDPQKSETPPGEKQKPKISGYWIYIVITILLIVFTFW
                     DFGPSAKETNRNVFEKQMLANNDVAKIVIINKEKVEVYIKPDKLSQEKYKEVSGSGMG
                     SPGKKGPHYYFMIGDVETFERQLAEVQASLPPENKITAEYRTERNYFGEILGWLLPII
                     ILVALWVFLFRRMSGGGGSGGNQIFNIGKSKAQLFDKEKHVNTNFNDVAGLDEAKVEI
                     MEIVDFLKNPKRYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFV
                     EMFVGVGASRVRDLFKQAKQKAPCIVFIDEIDAIGRARGRNPNFNSNDERENTLNQLL
                     TEMDGFASNSGVIILAATNRADVLDKALLRAGRFDRQIHVELPDLHEREAIFKVHVRP
                     IKLDKDVLLDFLAKQTPGFSGADIANVCNEAALIAARKNNKTVTRQDFLDAVDRIIGG
                     LEKKNKIISVNEKKTIAYHEAGHASVGWLLEHAHPLLKVTIIPRGKALGAAWYLPEER
                     QITTTEQMLDEICSTLGGRAAEEVFFGKVSTGALNDLERVTKQAFAMVAYFGMNDKIG
                     NLSYYDSTGQTEFSFSKPFSEKTAESIDEEVKLIVEQQYERAKELLTLHKEGIEKLAE
                     KLLEKEVIFGDDLEAIFGKKRGKEREIEEKPDVQTSGSPIDALQSESGQEGKLSETDT
                     SKHDDTEELG"
     gene            23938..24162
                     /locus_tag="PLK75_00865"
     CDS             23938..24162
                     /locus_tag="PLK75_00865"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOY37826.1"
                     /translation="MTTQKNWVKVYSTGVEYLAELLKQVLFDNGIEAVVVDKKDNAYL
                     FGWIEVYVSDDDYENSVKIVEDFEKNTSIK"
     gene            24137..24985
                     /locus_tag="PLK75_00870"
     CDS             24137..24985
                     /locus_tag="PLK75_00870"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOY37827.1"
                     /translation="MKKIPALNSMPQRALSGAVYVGMVVFCLWFSEYSMISLFLFLLF
                     LLHYEFNTLINRKKYKLPAIVTSVISISLLLATFIYEKTNGSTELFYIPLLLFVLLPL
                     LYILIDKEHDLRTLGVFLLSVIYFAAPLSLAVLISFRHTEEPVVTTSYQAFPVLSLFV
                     FLWVYDTFAYLAGTIFGKTPLIERISPKKTWEGVIGGVVMTLLLSLACSLIFNIFPIS
                     HAVAISLIVVIFGTLGDLFESWIKRSVAIKDFGNIMPGHGGALDRLDSFLFSVPAMWV
                     FYSLFG"
     gene            complement(24989..29983)
                     /locus_tag="PLK75_00875"
     CDS             complement(24989..29983)
                     /locus_tag="PLK75_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY37828.1"
                     /translation="MLHCSFLFSQQFNNEWINYDQQYFRIAVHTDGVYKISYNQLISA
                     GIPVDQIDPRNIQLFHEGEEQYIYIKGEGTSGIFDPSGYIEFYGKRNRAGIDSMAYTF
                     SEDIVNHDYSYFNDTASYFLTWNNTTNNRRMIVEDDTDFTQYTSNLIPYLWCIIRQNY
                     VSTFVKGSIRCIFGDGEGWFDNEILRKDHPDGDATVNKSLTVPYIYASGPSATLETAV
                     SGFAASAPENFFPHHLIVKLNDSPVIETQFLGYDYAVGTASIALSSLSTGLNLNFSAN
                     DVTLTSVPDQMVVSYIQLTYPRTLNAGNQSYFEFTVPAGSGGKQLLQLNNFISTDGND
                     VVFYDLDSHRRIKVVKQGTVHKALIPENGSERRCIMCGDNSLKQVDGIYKVSDNNKFI
                     NYHALYPQATYLIITHKSLMGSAQQYAAYRSGRGENVLIADVDQLYNQFAYGVQKHPI
                     GIRNFVFYLVNNNTVQPEYLFLFGKSVHCPDTRNDPNAYAYCLVPSMGYPSSDNLFTA
                     PFDGTGYEPLLATGRICARTNEEALIYLNKVIQYENNEPAEWMKNVLHFGGGANESEQ
                     TYFAGFLRNYEAIIEDSLFGGYVSTFLKNSSAPVQITQSDSVRNLINNGVSLMTFFGH
                     ASANGFDQSIDDPANYENQGKYPFVLANSCYAGDIHLYSNTCTSEEWVLIQDKGAIAF
                     LASVGEGLPSYLNFYSTELYNKVSRDYYGSPIGLQLIKAIQAAEVVFLTNVRMEITCH
                     EFTLHGDPIIAINAFELPDLTVKNSDIAFLPNEINTTIDSFDVQIVVSNIGRTTSSGF
                     TVNVNRTYPDGTMTEYVLGVDGCLYRDTINIRLPVNKIIGPGLNKLDVFTDAMNTVEE
                     FNENNNLTSISFLIRSSDLFPVFPYEFAIIPEDHVRLIASTGDPFLGDAQYIFQIDTS
                     DTFNSLVGLPLLQQTLSSSGGLLYWDVPMVLNDSTVYYWRIARNHTITDSIIWKESSF
                     IYIPHKEGWSQAHFFQFKNDRYQFIDYNRVNRAFDFVTVPRSLMVYNQRYVSPQTYSD
                     VRFTIDGGLNNGLGDHGCCGNQPAVIVAVIDPVTLLAYQSDYSNYGHRNYPQCGSSGR
                     VNYYFIFSSGWGTEYYSEGIQNMNNMILSIPNGHYVLFYSWTNAYFEQWEENVLSNFE
                     ALGSTQIRDLTNDQAYIFFCRKGSPSSAKESYSTAQNPYCTLQADLFTDFNYGYILSK
                     TIGPASAWTSFEWLQHSLESPSYDTAIVCIEGIRSDGTVDILIDSIKPDNYLIPDISN
                     TIAATEYPYLQMKFYSRDDLDKTPAQLDKWQLYFDEAPETAINVRDGYHFCCDTINEG
                     DEIVFAIATRNVSTVDMDSLLVKFWLQDKNNQIIPIATRRLRPHPAGDIIIDTIRLET
                     HGMSGLNSVWVEYNPINDLLGYYDQIEQHHFNNIAQKYFYVLSDEANPLLDVSFDGIH
                     IMDGDIVSATPEILITLKDENKYLALNDTSLFRVYLTDNENGTETRIFFNAVSSWYNM
                     EWVPAQLPDNSCKIIFRPVFNSDGTYQLRVQATDISGNESGDFDYLISFKIVTESAIT
                     NLLNYPNPFSTSTRFVFELTGSEIPDDFTIEIFTVTGKLVKVIDLFELGGVHIGRNIT
                     TYAWDGTDMFGDRLANGVYFYRVKARINGNSVDHRQSAADNYFKNDTGKMYILR"
     gene            complement(30081..31157)
                     /locus_tag="PLK75_00880"
     CDS             complement(30081..31157)
                     /locus_tag="PLK75_00880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOY37829.1"
                     /translation="MLLRTKLVILLAVLSNVVFSQTAPKYSNEFLSIGVGARSLAMGN
                     SVIASVGDVASLYWNPAGLVSLDRKIEIGAMHSEYFAGLSKYDFLGGAYKIDDNSALG
                     VSMIRFGVDDIPNTLDLIDGDGNIRYDRISSFSVADYAFLLSYSRTSPVQGLNYGANF
                     KVIRRIAGDFGGAWGFGFDIGAQYAMKNWLFGAQARDITSTFNAWSYNREIFEEVFEQ
                     TGNEIPENGLEITLPRLLLGAGYKFYIKNKFLIYPELGLDVTFDGKRNTLIKTDVFSV
                     DPHLGLEASYNNLVFLRLGVSNFQNLPDFDDTSTMSWQPNAGIGIHYKGFRIDYALTD
                     IGNQSIALYSHVFSLSYRFDLKKQ"
     gene            31242..31958
                     /locus_tag="PLK75_00885"
     CDS             31242..31958
                     /locus_tag="PLK75_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HOY37830.1"
                     /translation="MKTLKSLIPNLITSLNLCFGAAAIVFAFARQPETAFWCIIIAAG
                     CDFADGFSARLLNAYSEFGKQLDSLSDLISFGVAPAALVFVYFTDTADNGFPSWIAFN
                     AFTVALFSAYRLARFNISPPSSDFRGLAVPASALYLISLICFGAGLPKPISEVVLSAP
                     AVHIQVLIINLLMISSIPMFSLKFKNFNFTENLIRFIFLSVGVVLIAIFGIPGIWFVM
                     VFYIILSLATAIYHSKKTQL"
     gene            31955..32209
                     /gene="purS"
                     /locus_tag="PLK75_00890"
     CDS             31955..32209
                     /gene="purS"
                     /locus_tag="PLK75_00890"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOY37831.1"
                     /translation="MKFLANIDVMPLKALLDPQGKAVNNSLKKTGFESIENVRIGKHI
                     SFEIEATDQKSAEKTVDEVCRKVLTNPIMEGFTFVVVKTE"
     assembly_gap    32356..32455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32459..32534
                     /locus_tag="PLK75_00895"
     tRNA            32459..32534
                     /locus_tag="PLK75_00895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32494..32496,aa:His,seq:gtg)
     gene            complement(32572..34041)
                     /locus_tag="PLK75_00900"
     CDS             complement(32572..34041)
                     /locus_tag="PLK75_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37832.1"
                     /translation="MNAELKDRLKFRRQFIFVNKELSQFSHWEIIKTGKYFLYVHPDL
                     NYSVIEKAGKKAVLLGYIIDYRYPELSNEEIIGKYLDNATGKDSFIKNTNHAGGRWVL
                     YYSDINDEIMFNDATGMRQIFHFIGGDVTLASTEMLLSVATGEQKSAFIQKEFVQKII
                     NPDATWFPNTSTNFENIKCLISNTLYDIKNKKVSRFWPIERMELNYKDLIQNLHEILS
                     KTPNAIQKRYPLLALGTTSGSDSRISVGYFMHNCSKEFIMYTIQNRKLTDESPDIRIP
                     AALGRHLGIPHWVIRQTEEVDKDFEEAYRNNNANFKPTYYEMAYNLLAGYPQEYLSVQ
                     SVMNEIARQRYAYVPYNADLHDVRNFTDYGHLEFAGEEFKKWIVEPRQVYKDLKLQVE
                     DLFFMEQRMGRWNSANRSDWDLVQEVFEPWNSRIIWLIAMSIHRRHKQHTKNHVYRDL
                     FDLSWKAFNEVEINRGHQINYTRYRTKKLLSKIKRELLG"
     gene            complement(34063..35523)
                     /locus_tag="PLK75_00905"
     CDS             complement(34063..35523)
                     /locus_tag="PLK75_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY37833.1"
                     /translation="MANLKSVFKNTFFYSFSSIILRASSIIFFPIFSSYLTKADYGIM
                     AITQMIVTIIISTSCLQLPKGITRYLYEKDAEQENYEKRLMGSSFLIAILFNLILVAL
                     LIPFGSRLLSPVLNEIRFFPYMAYALATVPFMIVYEQYKGLLMAKHIGAKVFKLDMSF
                     FGSNIALNLLLVVVFKMDALGIILSNLICSFSFALYAILVYYRKTVLRFDFRLMKPVF
                     KFSIPLIPFFLAGFLLTSVDSIYLNRTNGVEISGIYYIALTFSTIFSMFKESFNFAFT
                     PWFYENFKENNHDYIRKLLNIIFWGAGFFCIGVSIFGYEILSLLSSNPELLEAWKYIP
                     LSTIGLLVIFIGQVYTMFVYHGKKNSKYLFISNFAGFAVNLGLCFAFTSFFNAYWALI
                     ARNMGFLVLSLIQIIIAMRSTEYRFDSLNVILVVLVCGSLSFIHYLPVNYVLLIFIKL
                     FIIGGFTFLLYRMLVNMKIAPVAMARDYISKLKTRK"
     gene            35635..36786
                     /locus_tag="PLK75_00910"
     CDS             35635..36786
                     /locus_tag="PLK75_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37834.1"
                     /translation="MKRVYHRGFLILSKLLLWVGYVCLPFIKLKKINSSNYAVLPYTP
                     ENWPGGKDRMAAWKPYFAEISAAYSIFWAWQEDEMQIILEYGGSENVVPAYKVYTKIL
                     IRRFRLLFSLRNYKSVWVQRAFVPSFPFRNAYFERLASFIHPYIVYDFYDADYIHNKN
                     LVDQTARLAFRVSVASKHLYNYFSNLNPATFFLRYAIIADHFIEKPTNNDAQTIHIGW
                     MGSPDNAKQLIEISAALSKLEEEFEHIVFSFTCRKLPKLPFARLERHSWGDEGFSYNT
                     WLAGLDIGIVPYMHPTETVKAKIAMKSLEFMGNAVPMVVSPYMHSDMLVHGRDVYIAS
                     TTDDWYEMLKALIISKEKRTAMGKAAKLIFDTYHSFRNNFEQLKQILEP"
     gene            complement(36843..37601)
                     /locus_tag="PLK75_00915"
     CDS             complement(36843..37601)
                     /locus_tag="PLK75_00915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY37835.1"
                     /translation="MEEINYNKVSKMYKVRSTLAGDHSVLIGSNDEVSLRSDILLDYL
                     TKRNLLWYLNPQPSDIILDFGCGIGRLSFLLQSKCKRMIGVDPSEELIEIASRRNNCN
                     NVEFRKIDFVYAPIELEAHVDKIFTVGVMYHISEEDLILRLKNFGALLSENGRLVFIE
                     HVSPTDRILGGVGVQRSMNTWMRLLTESGFSVKLSKPIIRMPSFSIAYWKKLKLTRNW
                     LLPCFFWLEKLSINRKPRLAEYYYYIFVCEKIRN"
     gene            complement(37589..38617)
                     /locus_tag="PLK75_00920"
     CDS             complement(37589..38617)
                     /locus_tag="PLK75_00920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY37836.1"
                     /translation="MNTSELKDTSIHITEMAPLQSEKWNAICNVCSNFFQTTYYDEVN
                     AGFKNKPVYFQVFKNEVLVAGIKIYTYQSGKPFLKSISRQAHAFGEPVINCNIENNDE
                     VLGLLAIEVLKYLNTEGYNYFKSKPVYGGYNLIFDSAINKKKPISFACLSLSNNLDTL
                     WDAIYKTHRRYILKAQKNGLEFAVEDNFDDFYRLLIETYAGQSIKPPDRRYLYDLYST
                     ALKYNAAEICFVKDKGEYLSAIMVIKFGKRVEYMHGGMHRNMLGSGHMIHWHMMMRYK
                     AMGFETAGFGQVADADPDNMKMGTVSDFKLKFGCSIEPSWQNEVVLHATRYKIYSGFN
                     NIRNIWKK"
     gene            38699..39874
                     /locus_tag="PLK75_00925"
     CDS             38699..39874
                     /locus_tag="PLK75_00925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37837.1"
                     /translation="MKKVCVLLTSSIHKDFRVIKTIQVLSRYCKVDLFYLRPSDSDKE
                     LFNENTRLFALKQRTGKKELFLRHTFFYNEFNYFFFDIIRHEIRYDYVWANDLPALKP
                     ALFLKKKFNAKLVYDSHEIYIETLNQFFPEKSHGIKKIVFGALLKLMRFYGSRAERAM
                     VEQTDIFITVSDSVKEYFAALFNRNDIAVVLNCPSLHQETYESVDLKSMLGFDNQVFL
                     LLYQGVMSYGRGLPLMIEAQKYTDQNIRFVLMGDGMLKNDLIELTNRLKLNEKVRFID
                     RVPTSKLLTYTRAADGGIILQETDKNLSKKLGIANKLFEYMHAGIPFVATNAPENNAV
                     ASKYDICIIVENNVESIADGINRLQHLNRGNVKEEALKASQYYNWEKQEETIKKLIV"
     gene            39946..40782
                     /locus_tag="PLK75_00930"
     CDS             39946..40782
                     /locus_tag="PLK75_00930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY37838.1"
                     /translation="MEIFEIKEKNIRICAKHLSIGTGVEFGKNINISVKETFSIGDMS
                     YIGPNTEMRGRNIVIGKYFYTSGGLRVGGGGRQHPGANLTVGDRCTFCNNLLNVCEPI
                     VIGNDVGLSEDVSLITHGFWLSALDGYPAEFRGITLHDGVIVGYRTIIMFGVEIGERI
                     VVGANSTVTRSLTKHGVYVGSPARFIKEIVPLTHEEKVKKTEHIISEYLKVAEYHELA
                     PKIEIQYPVVSVNNKFFVNFETLEFNGEEDMVTDDFRDYVRKWGIRIFTGRNMVSNFK
                     FD"
     gene            40784..41422
                     /locus_tag="PLK75_00935"
     CDS             40784..41422
                     /locus_tag="PLK75_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOY37839.1"
                     /translation="MINFQNKKILVLAPHTDDGELGCGGTIAKMLDEGAEVFYIAFST
                     AEQSLPLGFAPGTLRKEVEKATGKLGIKPENLIVFSYEVRKLNYVRQEILEELVKVKK
                     SHRFDVVFMPSLHDIHQDHTTVANEGLRAFKTTSILGYELIWNNLSFDTTCFIRLDEK
                     HIQAKISSLQEYHSQAGRDYTSEAFIRSLACTRGIQIGATYAEAFEVVRWII"
     gene            41430..42590
                     /locus_tag="PLK75_00940"
     CDS             41430..42590
                     /locus_tag="PLK75_00940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37840.1"
                     /translation="MRVLILADTEYTNDPRIIRQAASLQNNSFSVVVLCLKMKGENNT
                     GEIQGVTYQRFYDSQIHDIKAVTYHQQICDSILEQFEFDCVHCHNESMLNIAVMLKKR
                     KPVPIIYDSHELFHAWPLNVSSKSFSIRIKSIVIRRWAIRREKHNSRYIDYCITVCDS
                     LADNLWKYFKLRERPLVVRNIPQFTEIKSKPNILREYFNITEQTKILVFIGIHIYPKT
                     LNLEQVIDEVKNRENLALVFITTFNEGKSAIENYAVSVGADNVYFHEAIPPGQIPNYL
                     SSADVGLVPTWNKKDLSYWYALDNKLFEYMIAEIPVLATQQPEYTNIVDQYCIGVCVN
                     PDIKGSYAEGFNQITRNYSLYHENIVKSKQILNWENEEKKFLYFYQNLNFNA"
     gene            42583..43275
                     /locus_tag="PLK75_00945"
     CDS             42583..43275
                     /locus_tag="PLK75_00945"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOY37841.1"
                     /translation="MPKVISIHQPNYLPWLGFFYKMYKSDYFVFYDNIQIEHRSQQAY
                     VNRTKIKTNHGELWLTCPIHIKTGKLIQDVELVKTINWRKKHLKTLYYNYNRCKYFTQ
                     VYPGIEHLITHGSNNLCEFNIAIINWIIEYLDLPVKVLRASDIPVNETNPTLRLIKMI
                     EFLGADVYFSGNGARLYQQEELFEEHGIELRYTNFRQGDYLQCYSGFIPRLSVIDALF
                     NLGKETSSLLKQ"
     gene            43286..44320
                     /locus_tag="PLK75_00950"
     CDS             43286..44320
                     /locus_tag="PLK75_00950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY37842.1"
                     /translation="MITGYSIKVSSGKPSDRKRWDQIATENAQFWASTCFDQIQDIYH
                     KTPYYFEMFIDNTTMGGIKVYVSNYKRLKYISNRIFREAFIQGEIILDKKFSIDHLQV
                     KKIFESFLIYWFRKQNIVSVKVSPFNGNNELMPVFGKIQLEKRFNLLHINLNQPMDEI
                     FDKFHPKHRNLIRKAEKTGIRITEDYKPEMFYEQLLKQTYSGQEHKPTDKRFILAFCE
                     SAKNYGYLKMYFAWHNDTLLSGALVLVFGDYGDYSFGGNVKNNLGAGILLQWHIMKDL
                     KIRGVATYSLGQTADGYDTENEKFSVGISKFKRRFGGKELPVWSGCFVFKPILKAMKE
                     GLLKVFLNPK"
     gene            44328..45593
                     /locus_tag="PLK75_00955"
     CDS             44328..45593
                     /locus_tag="PLK75_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37843.1"
                     /translation="MKKILILAHDFPPYNSIGGQRPYSWYKYFFKHGLYPVVITRFWS
                     SDVINTEDFYKASSFHSISTEETETGTVIRIPVTPNLRDRFILKFGYRHSIIRKFLSF
                     FYMFAELIIKYFDPKSALFRQADTFLKTNHADIIIATGEPFILFKYASILSKKYNIPW
                     IADYRDCWSNNQNLGKIEKFVYSLFEKRFVKSSKCIVTVSNGYVNKIKDASRHPDIRV
                     VYNGYWTELIDEPCNKLKKDYFELLYTGTIYPYQNLEMFISGFRNFITNQPDAKISIS
                     FIGLEFFPEQVKRLQNLIVGIENYFATTPRMQLSDSFASMKNADAFLMLASKKRFQLY
                     AKVFEYIAIKKPVLMVVDDGNEVSRFLRDVNIGYFTNSEEDVCLHLTNLYNMWLNNEL
                     PMYDYSAEKFSRQVQAEKYATIIKQILLQ"
     gene            45629..46711
                     /gene="gmd"
                     /locus_tag="PLK75_00960"
     CDS             45629..46711
                     /gene="gmd"
                     /locus_tag="PLK75_00960"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOY37844.1"
                     /translation="MPKVALITGITGQDGAYLAELLLNKGYVVHGIKRRSSLFNTQRI
                     DHLYQDPHIENKNFILHYGDLTDSTNLIRIVQESQPDEIYNLAAQSHVKVSFETPEYT
                     ANSDALGALRLLEAIRILKLEKKTRFYQASTSELYGLVQEIPQTEKTPFYPRSPYAAA
                     KLYAYWITVNYREAYGIFASNGILFNHESPMRGETFVTRKITRAATRIAMGLQDRLFL
                     GNLDSQRDWGHALDYVKAMYLIMQHGKPDDFVVATGVTTKIRDFVIMAFAELGISLEF
                     RGIGVHEKAYVAECYNPAYAVKKGQCVLEIDPGYFRPTEVELLIGDATKARTELGWIP
                     EYSLDLLVKEMIAHDLKLLKNNQNYF"
     gene            46713..47642
                     /locus_tag="PLK75_00965"
     CDS             46713..47642
                     /locus_tag="PLK75_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOY37845.1"
                     /translation="MDETSKIYVAGHTGMVGSAIMRQLRSKGYSNLCYKDITELDLTN
                     QQQVLEFFSAEKPEYVILAAAKVGGIVANNTYRAQFIYENLMIQNNIIHQSYLHGIKK
                     LLFLGSSCIYPKLAPQPLTEDYLLTGELEPTNEPYAIAKIAGIKMCEAYRDQYGCNFI
                     SVMPTNLYGPNDNFDLNNSHVLPAMLRKFHEAKLNNSEQVVLWGTGSPLREFMHVDDM
                     AAACIFLLEKYNGRQFVNIGVGEDISIKELANVICKITGFNGTIVWDTTKPDGTPRKL
                     LNVNKLHSMGFRHSIALEQGIESVYKNVFLSNS"
     gene            47639..48916
                     /locus_tag="PLK75_00970"
     CDS             47639..48916
                     /locus_tag="PLK75_00970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37846.1"
                     /translation="MKKVLLLAYDFPPFNSVGAFRPYAWMKYFHEFGVYPFVVCRNWD
                     EIGHQPSLYTKPSLQKEAIVETTPVYTLIKAPYRGNFRDRLIYSHGIKSGLIRKLLSL
                     IYSIWEFHSLRCDARSEIFYEAEKVISNNKIDFIIASGEPFVLFRYAHLLSKAHNIPW
                     IADYRDAWTTNFNMYAFNPVERFIKAKIIRRHEKMIVSSAHLITTVSEGLVTNLRSIH
                     FNKEIKIIMNGYFHEDFEELKKQTSQNKKMVIAYSGSLYPYQRLEVFLDALFTLIEIS
                     GELPVEVKFYGMKLHPERKKSIIEKHPQFEKYCSFIPVLPRRELLEKLVEADVLLLLA
                     NEKIDGSCAKVFEYIVLQKPILLSVNDHATLETLLKSNKNAYLCENTAEVANALRSLL
                     REKTETGQIQSFEFDYFAYSRRNQTEKLAALLQ"
     gene            complement(48934..50388)
                     /locus_tag="PLK75_00975"
     CDS             complement(48934..50388)
                     /locus_tag="PLK75_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37847.1"
                     /translation="MPYTFMLKKYSKAIVVIVLTALLVRVVFVLFGAELYYGKQNFFE
                     TGDTYSYTQCAKNLAEHGTFRGILDDPKSVAARLPAYPLLMTAVYLISGNDVDLMYQI
                     MIWIQIVLDVASVFILYQLCYFISKSYRLGIVTALIYALYPFAIIWVPLISSETMGIF
                     TMLLGLMFLARKRNKTDFLFGGVFIALSALCRPQNLIFIPVFVFVYLLYNIRNLKFAL
                     LPITLALIGFSGVYGSWITRNYILLDKFIPFSDSRGGMETFSKDVVACRKYIYSLQSD
                     WNPQFFQIVRNEKVEWPEIAFVSKEDSLMLLRAEYLAKNCGYGFSYWREYWNKDNAQI
                     PIEESCCDSIVYYFSKLRTNQIKAHPFHYKIIIPLQNLRKALFKTSMVNEKKGIFGLA
                     ITMLFLYRSLLILIGFIGVYLMLRKKNLYGLFIFLFFAVVYYYICFTVRNIEMRYFLN
                     NDILMLVPAAWVIVNIYKWFAIKRHSRKPEQFAV"
     gene            complement(50427..51854)
                     /locus_tag="PLK75_00980"
     CDS             complement(50427..51854)
                     /locus_tag="PLK75_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37848.1"
                     /translation="MFNIEGFRRTFDFYYTGWDFTFLLLLLIAIWAFGMFIRQRNIKE
                     HPYYKYYPLGLMVKVLGGVLFCLVYTGFYNGGDTTLYYWSAKALVNLAQHDFDSYLKI
                     LLGDRSPEALSAFNSITEFPFYTRDPKAFAVVRFTSIFVFLGGKSYLWTTIFLNIFLF
                     GGFWKFYKVLCSLYPHLSKTLAYGLFLIPSVVFWGSGILKDSYTMAATLWFAAGFYNA
                     IIKFDRKRLVGNLFYIVFSVFILISLKPYILMTLILGMTIWFSFGYIQKIKSKFIRLF
                     VFPVILIVAALGGTVAMTRFSSAAGEFYSSPEAMMERAAIVQNDLTQDYYGGNSFNIG
                     SFDASYAGILSKAPIAIVSGFFRPFPWEARSPFNMLSALENTFVFALLMFAIFGRGIK
                     NFGKQLTGNHFVIFALTFSITLSYIVGLTTANFGALVRYKIPYMPFIFTALLITYNNF
                     RAERRLEREKPEVESVKSDVSAPVV"
     gene            complement(51866..52981)
                     /gene="rffA"
                     /locus_tag="PLK75_00985"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(51866..52981)
                     /gene="rffA"
                     /locus_tag="PLK75_00985"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOY37849.1"
                     /translation="MIPFNKPHITGKEVHYIYDAVASGKISGNGKYTKKCQSFFEEIW
                     GFKKTLMTTSCTDALEMAALLLDIKPGDEVIVPAYTFVSTALAFVRQGAKIVFADSRP
                     DHPGIDETGIEKLITPRTKVIVPVHYAGVACDMDKIMNLADKYKLWVVEDAAQAIDSY
                     YKGQPLGRIGHLGCFSFHETKNIQCGEGGMLAINDERFMKRAEIIWEKGTNRAEFFRG
                     EVNKYGWVDIGSSFLPSEVISAFLWAQIEDYKNIQEKRKAIWAYYYDTLFNYQTNKFS
                     LPVIPDYATNNAHMFYIICNSEAERIRITGNLKTNDVLAVTHYLALNKSKFYSKIESP
                     KDLVNCDRYESCLLRLPLYFDLDNSNLQRICKVINQS"
     gene            complement(53005..53667)
                     /locus_tag="PLK75_00990"
     CDS             complement(53005..53667)
                     /locus_tag="PLK75_00990"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37850.1"
                     /translation="MKKRLIIVGAHGSGEIAMTVFEDINQQTGEWEIAGFINDVVPVG
                     NYLGKHKVIGNTESILDFVNNGYYIHYTYHFNAKMKNRRVIDFEKLNIPLEANATGIH
                     PQAYINPTSKIGCGSLILPYAATSAYAEVKNFIHVYTNGFIGHNTIVEDYATVAAHGV
                     LGARIRIGTGAHVGLNSTVREDIKVGDYSIIGMSSVVVTDVEPFSIVAGNPAKLIKKI
                     QE"
     gene            complement(53664..54587)
                     /locus_tag="PLK75_00995"
     CDS             complement(53664..54587)
                     /locus_tag="PLK75_00995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY37851.1"
                     /translation="MNTFSVIVPVYNSEHTLKPLTERLVAVFNNLRSEFEIIFVNDCS
                     NDNSLQVLKEIRTIFGKNIIIVDLYRNFGQQNALMCGFQFCSGNFVVTIDDDLQNPPE
                     EIPLLVEKINQGYDAVFGCYEKKKDKFHKNLGSRILRKLNHAIFNVKGDLRFSSYRII
                     KKDIIDQIKSLRTPYPYISGMILETTSKVTNQIIKHEARQHGKSGYSLKKLVNLSFNL
                     LINYSSIPLKIFGYLGLFVSVISLIISGVFVIKQLFLGNAPAGWTSLIVLISFYNTLI
                     LILFFVFGEYIKRILREISNYRPYIIKDVIK"
     gene            complement(54589..55365)
                     /locus_tag="PLK75_01000"
     CDS             complement(54589..55365)
                     /locus_tag="PLK75_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOY37852.1"
                     /translation="MDAEKNIIQEQEMHFIAMAETLKKKFTSRRINFYACSSVIEAQK
                     TLTSLVEAFKSNTPPTQVGFSDSVTLHQMNAYDTVGKIPGLSINNPLERNNQGIYTIF
                     GDQPDGKLNLPKEEYYALMEKLWQKMRETLLSDIFIIGANAITMNGQIVSTDGTGNRV
                     GGMIFGPRKVIIVVGRNKITRNLEDAIRRNRDIAAPLNYHRHNIKHHNRFDNPCMKTG
                     YCSDCNSPRRGCLKTVIIDGEMEGYKDRTHLILINQDLGL"
     gene            complement(55378..56670)
                     /locus_tag="PLK75_01005"
     CDS             complement(55378..56670)
                     /locus_tag="PLK75_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37853.1"
                     /translation="MLLYLLKYYNQISIWKRLDSPEIRRRQIIRFNAIFQHAKTHSAF
                     YKDLYQKHQVFNLEIKSMKDIERLPVIDKSVLRNYSLQDIATVDVSNPRKFNIRSTSG
                     STGEPFRIAYNKREDFTNQVRHFWSLRQYGYQPLKKVLIVTRYEPDAVYQYEKDLKWF
                     RTIQKLLPFFRRQIVSVYEPIEKIVEKIQKEKPYILWSTPSMLQIISYKMQEKGIKLN
                     TPYVCFTSETVFDKQAQLFYDTLGKNLVNYYGCMESPTIGFDINMENKFRLFPNSAMF
                     EFIDHRTENNQHLATPVLTNLINFTMPIIRYNTKDIAYVEDHPDFGVKYLGKFIGRLD
                     DIFEFPDGTMFAHHHAHEMFMDFHECEHFKFVQYPDKSITLQLKVKNSVDKNEIKAKA
                     LQRWQKRYPDKIISIEFVDEFPIDTQSGKTRNMERIKN"
     gene            complement(56670..57560)
                     /locus_tag="PLK75_01010"
     CDS             complement(56670..57560)
                     /locus_tag="PLK75_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOY37854.1"
                     /translation="MNHYLLRSLMFVPGHNERLLSSASRSEADVLLLDIEDSVQPVAN
                     KALARTMVKQWVSEGKFKNHLVFPRINDRESGHLLKDVEALSIEGVDGFMYPKAKTGQ
                     DIYFFDKLLETIEYEKGLPVGKFKIIPLIETAAAVLNAQDICKASDRVIAIAFGCEDF
                     ISDLEGIHDPEHESLFTARAMIAMAARAVGVIPVDTVHINVHDLDDLEINLRLAKKLG
                     FEGMLILNPKEIALAHKYFSPSEQEVFDAKEMLRLSEEAEKEGKGVALMNGKFIGPPM
                     VIAAKKVLVKHTKIMNKHHV"
     gene            complement(57839..59125)
                     /locus_tag="PLK75_01015"
     CDS             complement(57839..59125)
                     /locus_tag="PLK75_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY37855.1"
                     /translation="MNFQFKNTVTPERKKSLINFGYYASTTMIGEALPFLLLPLLTIY
                     LSPVDYGYINNFRALFLISNALIGGGLSSTISKFFFNRDDEYKRVQMFNLFLILLIIA
                     GSGVLLLFVISFFTEIQLIPEPLFVIIPAISFFFIGFEFYTVRLRMTKQAKLFAGINI
                     SRSVIDLSMSLLLVIAFHMNWAGRVTGTIVSIIIMGLLAILLLWKKNNIRVLPSKQHR
                     EDILKNSLSLWPHALATVIIIESGKIIINTYLNKAEVGLFSVGSTIAMVIFFLGLSFI
                     NTWSPIAYERLAKQSFRKSRDFIVKSQIFIAISLFILSIVLSFVSPWIISLMTTPAFA
                     KAAVVIPWLSFAMSFRVLFTANSSFFVHYGKQRYMSYSTVLGAVVFVPLSIFLTQTYG
                     IVGCAASICITYFLMEIVCLSLFISTYKKLALQNLK"
     gene            59222..60748
                     /locus_tag="PLK75_01020"
     CDS             59222..60748
                     /locus_tag="PLK75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37856.1"
                     /translation="MKAIYNTCVSDPFLKVAIQLCEKYNIEPIYWIGYHNDASEQDVQ
                     ITFPDIIYHRYYDAWTGILPEKTRHFADETPEDIDFINSHCFEEINALSIMDRMDPDR
                     FSFPFAERQMLYRKYIRYWTGFIKSKKPDVLISAIYPHRIYDYPLYALCRHFGIKIIS
                     FYPVSIPSRIIPINSIDNFGTKIKEKYRELISSPNADLSLSKDIEGYFQKLRGAYDTA
                     SPYYIKINANRDKQTSNVFKAAWVFLKNPKFYNEKNTKILFHGIELLFKQKNKDVEDS
                     KRYPFIAYMRILLKKRLMQKRLRKFYDSIAQKPDLTQKYIYFAMHYQPEASTTPIGGV
                     FTEQLMAIRQLSAAIPDNWKIYVKENFYQFVNYSHGHLGRIKKHYTDALSIPKVVLVP
                     TSIPSFELADNAMAVSTITGTVGIESVVRGKPVIVFGLTWVEDFDGVLKITSNKDAER
                     IMPFIQSFVHDEHKVNCYIKAFDMASVYAYHYPGKKLRFNMDEELCVKNLIESIAMQV
                     "
     gene            complement(60745..>61530)
                     /gene="pseI"
                     /locus_tag="PLK75_01025"
     CDS             complement(60745..>61530)
                     /gene="pseI"
                     /locus_tag="PLK75_01025"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HOY37857.1"
                     /translation="QEAAMPYEWQPKLKKVAEDFGMLFFSSPFDLEAVDFLESIGVGM
                     YKIASMEITDIPLISYIASCQKPIIISTGVAEPDDIELALQTCYLRGNHQVSLLKCTS
                     EYPAPVEKANLLTIPDMINKYKVPVGVSDHTMGSIVPVVAVSLGAVIVEKHFILERKL
                     GGPDSGFSMEPHEFKEMVNTVRLAEKAKGEISYVVSEKDKSRRRSLFVVKDIGKGETL
                     TSENIRSIRPGAGLHPKFIEEIIGKKARTNIQKGSALSMDDVD"
BASE COUNT        18662 a        12215 c        12167 g        18386 t
ORIGIN      
        1 aatgaagatt gtgaatgctt cgtgcaattt ttcaagcaca gcattaacat tacacgaagc
       61 attcacaatc ttcatttgtc ctgattcaat tttagaaata tcgagaacat cgtcgataat
      121 acgcagcagg cttttgcaat tattgtcaat aatttcggag taataggttt ttttatcatt
      181 cggagtgccg ggatcggtca ataaattcga aaatccgaca attgcattca ttggggttcg
      241 tatttcgtgg ctcatatttg ataaaaatgc cgatttgagt ctatcgtttt cttcggcttt
      301 ttctttagca gcaatcaatt cattctgaac tcgtttaatt tcctcaatat ccctgagtat
      361 agaatgaagg cttcggtctg gcattaccct ggtgttcatt tcaataggaa taagctgtgc
      421 atctttccgc tttaaaagac gctctgacct aacgctttta ccttccatca gtaaatcgta
      481 tcgcattggt acacgcgata gttcttcaac agcaaacagg tcttcaatac gcattttagt
      541 gatttcctct ttggtatacc ctgtcagcat ttcagcctga tcgtttgcac ccacaattct
      601 tccgtctgcc gacccgagca atattgtatc agcagccatt tcgattatcg tccttagtcg
      661 agcttcgcta tttaacactt ttttctctgc cagaacgcgt tcagttatgt cggttgaaac
      721 tcccaaaact gccttatcgg tatcattata gtttgtaaaa gggattttta tagtctgaag
      781 gactttatcc ggttggtttt ctgttcttag cacctcttct ttaataaata gttctttccc
      841 gctttttatt actttcaggt cgtcggagcg aaaatgattc acctgttttt tgtccttcac
      901 aaaatcggaa tctgttttgc caattaaatc ttccggagtg gttccgtatg ctgacgcaac
      961 cgccttgttg gcaagaatga atttaccgta aatatctttt gcaaatataa aatggggtac
     1021 catatcaatt atctgccgta actgtgcttc acttcttcta aaagcttgtt ccgtagaaat
     1081 tccctttaaa aacaatgtaa tctgacgtag atacacttca tactccgtaa atagctttat
     1141 ttcaactccg cgtggtgtaa aaaagattat gtcggcaagc agtgaattct ggtttttaat
     1201 gcgtatagct gcaatattcc cgacagggat agcacattgt aattttcggc taattgcccc
     1261 tggaacaata aatcgaggta tttcgaggaa agatttatat gaaaaggact cctttttatt
     1321 aaacggtgta tcggaaagtg ggttatactg ccaacggcgg cctaaaattc tgaatccaag
     1381 aatgttttca acagctttta ttaccccttg atcacctatt accgaaactg tagagtattc
     1441 ctttgttgac tcattaaact ggtttaatgc acaaaacgta gcaccgctgt attcaatgag
     1501 ttttcgcaac aaggcgtcga tgttttcatt ttctgaatac agttgcaaca ggtcgttcga
     1561 aaaggaatta atgtgctcga gacgtgtgtt cttttctatc ctgttagtaa tatcacgtgc
     1621 tgcagtaaac aatttgttac cataggcaaa aacacgccat tcaaagaatc ggtactcgcc
     1681 gtttttacaa aaaatgcgat tggtaaaact gctgattccc gtatgctgtt taatggaatt
     1741 caagcttgca tgccattcat tcacatcgtc gggatgcaaa ttatctgtga attttcgttc
     1801 gagaatttcg cttttcttat accccaacaa cctttcccat gcagaattga tttttaatag
     1861 ctgctgcgaa tcccctatta cactaagcag atcgggtata agttcaaaga agctttccga
     1921 ttcattgatt tgttcctttt cgactgagat gtcggagaat gagtacataa aaaaattttc
     1981 atcaatgctg gtaaagtcta ttcgatagca tttttcttca gaacagtaat agcctggttt
     2041 caaaattcca gtacggtttt cgacagcaat ttgcagtgcc tgaattattt tctctgcctt
     2101 aggcgaaatt gatttatttt tccggttaat aaataaacca tgtaataatt ccctactgat
     2161 acctgaaagt gtttcgaaag attcattagt atcatcgatt tgatacagtt ttttatcccc
     2221 acagagaacc caatgtcccc aaatagccgg tgctggatgt ttatagaaaa gtttttgaaa
     2281 aatactccct tccgtgttat taattaattg cattattacg aatgtttaga tattaaatat
     2341 aggcatagcc atcaagcata tcgaaaaggt cgcgaaagtt tatgggtttc gatatatagc
     2401 tgttacagcc cgatgatata catttctgct tatcttcggg catcgcaaaa gctgtttgtg
     2461 caataataat cacctcggtg ttatgctttc ggattatacg agcaacttca taaccatttg
     2521 ttccgggaag ctgtatatcg agaagtatta catcgaaatg attcttcaat gccatattaa
     2581 tggcttcggt accatcaaat gcaaattccg attcacagtt atcgtaacgc ttcagaattt
     2641 ctttcaaata ctgagaatta attaaatcat cttcaacaac tagcaaccga agctttcttc
     2701 gttgtttatt tattttcccg gctttattct gttcaacgca aacctcaacc gcctgaagaa
     2761 atggtattgt aaatgtaaat ttgctgcctt tgcccttgat tgattttacg ccaatttttc
     2821 cacccatgag ttcaacaagc ttcctacaaa tcgacaatcc aagccctgtt cctctggggg
     2881 gaccggttcg tgcaatctcg gcctgccaaa atcgctcaaa tatcttgtcg tgcagtttct
     2941 tatcaatgcc aattccagta tcttcaacaa aaaactcaag cattgattca tttatatgaa
     3001 accctatttt tatatacccg gtttccgtga atttaacagc gttttcaatc aaattattca
     3061 aaacctgcaa caaccttgtt tcatcggttt ttactaaaac tgtctgtgaa ggttttttgt
     3121 aaatcagtcg tacagcatta cctcttttct cggaaatcat ttcggcaaaa actgatacaa
     3181 tattatcgaa catgttgttg atatcgactg tagaattcac aatggtaagc tgccctgact
     3241 ctattttcga gatatccagt atatcatcga ttattctcaa cagactgaca cacgaattat
     3301 tgattatttc ggagtaatac tgtctgctcg attcatgcat tcctgaattc agcattagtt
     3361 gtgagaaccc cattattgca ttcatgggcg tacgaatttc gtggctcata tttgcaagaa
     3421 aggcggtttt taacttgtcg ctttcttcgg ctcgttgttt ttccaacaaa agtgcctttt
     3481 cataaacaat aaggtcggta atgtcttcga gtgttacata caccgatttc acatttttgg
     3541 tgattttgtc tttatttgga attacgttaa tattgagcca tttttgcctt gaaaggttac
     3601 cccccgaaat acccatagtt atgtttttta ctcttcgacc cgttttaaat gcttttaaca
     3661 cagggtaatc ttccatttta aacggctgtc cgttaagatg agaggttgtc caccccaacg
     3721 aacccgaagt aatagacctg ccttctaagt tatcctcttc aaatattcgt tttgccgaca
     3781 aatttgctga gatgatttct ccggaattgt cgcagtatat cactccctga gccatagttt
     3841 cgtaaagagt acggtatttt tcttcactca tgctcaaacg tctcattgca tgttttcttt
     3901 cggaatcgtc gcggataact gtatgcaaca tggtcgaacc cttgtaaata atgctggtca
     3961 gaattatttc acattcaaca gttgagccat cagcacacaa atgggtccag ccaaatttat
     4021 ggctgccctt tgtaaatgca atattcatca tttccgttgc cttatcctcg cttaacagac
     4081 catcttcctg ataaacaggc gatattaaag ccggtgattt gcccaacaac gatttcttcg
     4141 atttgaattt gagtagcttt aaagcagcct tattgcattc cataaattta ttgttgctca
     4201 gaataagcgt tgcttcggct gactcctcaa aaattttccg gaatcgttct tcactttctg
     4261 ttaattcgtt gatcataacc ctggttgggg ttatatcttc gaaaattgtt gcaaaactat
     4321 tcggtttgat agcaacaacc gaaatctgaa aatatttttt taatggagca aaataggtct
     4381 cgaaaaagca tgatttccct gtgagagcaa cctgtgagaa ttcgtttagg taaggagggt
     4441 ccggtgtttg atatatttca ttcgccagct tattgtaccc tgcaggaagc gaaatgcctg
     4501 tgtgaacctg atatgctggg ttggctttta ttatacggta attggagggt gctccttttg
     4561 tatcgaaaac caaatcatga attgccacac cttctttcat attttcgaaa aggctgcgaa
     4621 aacgaccttc gctctgtttg agcttttctt cggcaagata ttgtttgctc acatccctga
     4681 aaaccagcac aactccaata atttgtttat catccgaaat tatcggagca ccggaatcgg
     4741 cgatatggta ttcggtgcca ttgcgcgaaa ttaaaaccgt gtggttggcc aaaccaacta
     4801 cacttccggt agtaagcact ttttcaatcg gattttcagc agcttccctt gtgaacgcat
     4861 tcacaatatt aaaacaatct gatatgggtt gttcaaacac ctcctccagc atataccctg
     4921 taagcttttc agcaatccgg ttcataaagg aaatattacc tttagtatca gtgcagataa
     4981 tggcatcgcc tatcgactgc aatgtggtct caaatatttc tttgcgtttg agaaggtcgt
     5041 cggtgtttgg ttttgcattc attccaacat ggtttgggtt agagcataaa attacagata
     5101 aaagcaatgc aaatcaagaa atttgacaga atcacactga gggttaatta ttggggcaat
     5161 aaaagcaatt taaaatgtcg cgatgtttgg aatcagaatg caggaggcag aatacagaat
     5221 tcagaagggg acatttcgtt gtgcgataga ggcatgatgt tacgagagag aaccaaacct
     5281 ccagcaagag aataatgctg ttgaattgcc agtcgcccct tctgtattct gcattctgta
     5341 ttcacaaaag taataatacg ccattggttg taattgagcg gagcaaagtg gtaatttatg
     5401 ctgaggcacc cgaagtatcg aagggtgact gctgaagacg gtttttcatc cccctgccta
     5461 tgcttcgatt aatcttccgc tacgctccag tactcagcat gacaggggaa ttgtcaccct
     5521 gagggctaat tattggggca ataaaagcaa tttaaaatgt cgcgatgttt ggaatcagaa
     5581 tacaggagac agaatgtaat aatacgtctt tggtagcaat ataaaaaaca gggttgctgt
     5641 taaaaatact tctgcctctt aagtaaatat gccgtaagca catcggagaa atcgctgtta
     5701 atatcggctt ccacaaaatc gatttggtat tggccgcatt tgagctttag ctcattaaca
     5761 tactcctcaa cgtttttcac gtaagtgtct ctaatttcgt tggggttgag tttaatattg
     5821 ccacctgttt ccaggtcaac aaatttataa ggtcggttgc tgaactggaa ttgctgctcc
     5881 agcttgtgat cggtaacatg aaacagaatc acctcatgct ttgcatatct caggtgttgc
     5941 aatgcaggga acaacaactc ggggtcgttg cttccaaaca tatccgaaaa aatcaccacc
     6001 agcgaacgct tatgcattgt ttcggcaagc tgatggaggc aggttgctgt attggtcgtt
     6061 ttcttcatgg gtatattttc gctgttcatc agcaaccgta gttggttgta catcacctga
     6121 acatgaacct gcgataaacg gggttcggag tgaaagatca cctcatcgct gaataggctt
     6181 aagcctaccg cgtcgcgttg ttttcgcaac aaatagatga gtgatgccgc acaatacaca
     6241 gagtacagca gtttgtttgc ctccttgctt ttataaggaa acagcatcga cgacgacaca
     6301 tcgataatga tttgcccgcg aagattggtt tcctcctcgt agcgttttac aaatagtttt
     6361 tcgcttcggg caaaaagctt ccagtcgata tgttttgtcg attcaccggc attatacagc
     6421 ctgtgctctg caaactcaac cgaaaaacca tgaaagggac ttttgtgcaa tcctgtgata
     6481 aacccttcca ccacctgctg tgcaatcaga tcgaggttat cgaactccct aatgtgttct
     6541 atgtcggata agtttttcac gcgatattaa taacggtgct tacgggttta tgttgtctga
     6601 tacgaaagat tgcacaacgc atcagccagt ttatcgagca tttgttttgc cggcttttca
     6661 agcatattac gcataattac gttgagttct gcttcaatgg ttagttttat tgctgtggat
     6721 gagctatcga cagccttgag ctgaatccag aaaaaaaact caaacgggtt ttcggccttc
     6781 gaagtgatct tgatggtttt gaatggttct ttcgctgcaa aaatcagaga aatacgttga
     6841 cctttggctt taaaggaaca actttctgcg tctgaagtcc attcttccag atcgggtgga
     6901 atgaaactat tgaaattcct aaaatccgat aaaaaattaa atattttttc atcgtgttgc
     6961 ggaactttaa ccaccttgct ttctacatga aactctttca tagtgcatga aacttatttt
     7021 ttccacactg aaggattcac cctccattcc tgcagtgttt tcaactgttc tgccttaata
     7081 tagccggttt caatggcatg ctctatcagc acatcgtaat tgctcagggc aaccagtttc
     7141 acgttttcgt tttcgaagct tgtttttgcc acatcgaatc catacgaaaa aattgccacc
     7201 atgccaacca cctcgcaatt ttcggctctc accgctttta ctgcgcccag actgcttcct
     7261 cctgtcgata ttaaatcttc gacaaccagc acttttttgc ctttttcgag atagccctct
     7321 atcagatttt ccaatccgtg atctttgggt ttagaccgta cgtaaacaaa tggttttttc
     7381 aaggcatcgg ctactaaggc cccctgagca atagctccgg ttgctactcc cgctaccaca
     7441 tcgaaatcgc caaaattcat tccgatgatc ttcacaaaag aatctctgat gaatgtacga
     7501 acttcgggaa atgataaggt tttgcggttg tcgcaataaa taggggaata ccaccccgat
     7561 gcccatgtaa agtgatttgt agggtcaagt tttattgctt taatttgcaa taattgttct
     7621 gcgatttgtt ttgctatgtt ttgcataata caatggaaaa aatttatttc aaaaatcggt
     7681 ttataagtat ctgtgaatca ccggttaacc gcaaaggggc catgaacatc aactaccaaa
     7741 ccgttcacga cctaaatata attatttata agtttttggc aaacaatcat atttcgagct
     7801 tgcacatatt tggaaaaaag ccaaaaaaaa tacttaaaaa gataaaaacc gtttttcatt
     7861 acataaaagc tgccggaggc gtagtagtaa acgaaaccga tgaaatcctg ctcatcaggc
     7921 gtcgtggtaa atgggatctg ccaaaaggta aaaaagaaaa aaaagaagat attgaatcta
     7981 ccgccatccg agaggtttgc gaagaatgtg gattgaatcc cgaattattg aaaattgaaa
     8041 actttatcga caacgctttt cacatctatg aagagggacc cgatttcatt ttaaagcgca
     8101 cctcctggtt tgttatgaaa tattcaggca ccgaagttct aattccccag accgaagaag
     8161 atatcacaaa ggcaaaatgg gtaaaaccag acgagctatc gcgttacctg aagaaaacct
     8221 ttttaaacat tactgtaatt cttgaaaaat acctgaaatc aacaggggtt attattgact
     8281 aatacaatta atcgtcgttt atcaggctga aaaatgcttt ctcaaaacct tcgaggggac
     8341 ccggagccgg tgaataaata aggaaacccg aaggactaac caaaaaataa ctgggatatg
     8401 ccacaatttt gtaatattcc ggaatggttt tatcaccacc ggtaaaaaca aaatcccagg
     8461 tatatttctg ggcattcaaa tagcgtttgg ctttggcaac atccgaatcg accaccaccg
     8521 ataccacctt gaaatactga ttgtgttttt cgtaaatggt tttcagtaat tcgagctgtt
     8581 gctgacaggt gtatgaatcg gtattgataa aattcaaata cacaaattga ttccggtaat
     8641 tcgatagttt aacagtgtcg ccaagcgtat caggcagtgc aaaatgcgga gccaccgtgc
     8701 cctctgccat ggtgagcact ttgtcggcga tgttttgccc aatgaccctg tgttcgggat
     8761 atgctgtaac gatgcataat gaatcgagac aaagcagcaa tggggtccac gcataattat
     8821 cattataaaa agcatcgtgt atccctttca gaattgccag ctccttaaga cgggggttct
     8881 gcagctcaag ccagtacgaa agcaagagtt taatgtttgt gatgctatgc ccgtagttta
     8941 cttcccagaa caacctggga ccaattaact cattcagttg gttatcgaaa aaattgctgt
     9001 acagctctcc gaaaatctcc atatatgccg gattttcgta gtacacacta tcgaaagtga
     9061 ggtttttaaa tgccacttcc ggtctgttgc gttgatatgc caagtaacgc aatccaacat
     9121 atttgaattt tttatatgta ttaaaatacg ggttgccttt ataggaaaaa tgcgagtcga
     9181 tttcattaat gaacgtgtca acatccgact ccatgccttt tacataaatt tcaaacatcg
     9241 attcatcgac atattcatcg tagaaataat cgaagtccat aatgaggtaa ttcaaatcgg
     9301 tttttttcat gttttttatt ccgatatgca ccgattcggg ttcgaaaaag gggtttaact
     9361 catctgcctt ggttttttcg atatattcag ggagtataat atcgtaattg caaccaggtt
     9421 ccatataaaa atatgcaaaa taaatccctg tttcggcaaa aaccatacag gttttatcga
     9481 gttcacattg ccctgagaaa taccccttgc tgtcgacggt aactgtaaat aggtcttgtt
     9541 ccagcatggt gatgcggtcg tagaattttt gaaatacaat tttttttccg gcataggcag
     9601 gagccgtacc ctcaaccttg caaataccgg catacagatt tgttgtaaaa acagaaaaga
     9661 atagtaaaaa ggcaatattt ctcatcatac tcagaaccaa ttgcacaaaa tgaataaata
     9721 gttttgcgta aatatacttt attaacaacc tgattttatg ttttattgta aaaaatccga
     9781 aattcatcca taacaattaa tcatatcatt tttttatgct ttcttcgtat gtttgtacca
     9841 aattttagta attgaaagtc atacgtaata taattcttac tgccgtcgcc tttgtgtcta
     9901 tcatatcctg tgccaaaatg ggcgcgctga gcggcggacc cgaagacaaa gagcctccgg
     9961 ttgcagtatt tgccgatcca ccgaatgctt cggtaaattt tgcagcaaca caggttaaaa
    10021 tcaggttcaa cgagtatatt attttaaaaa atgtacagca ggcattaatt atttcacccc
    10081 ccatcgaccc caaacctgaa gtattggttt cggggaaaaa cctgataatc aatattgaac
    10141 ccggagcatt gcgggagaac acaacctata atcttaactt tggtgatgcg gttgccgata
    10201 ataacgaagg aaatcccctg aaaggtttta cctattcttt cagcacaggt aatctgattg
    10261 attcattaat ggtgtcggga acactggttg atgcgttgac gttgaaaccg attgaaggtg
    10321 cttccgttgt actttacaaa gaagggaacg acagtctatt tttcaccacc attccccagt
    10381 atctctcggt taccgatgcg aacggaaaat tctcgcttca atatcttgcc gaagcaaact
    10441 acctgattta tgccataagc gatgccaact acaattatta tttcgatcag ccatcggaac
    10501 ccattgcttt caccgatgca ataatttatc ccacagtatc gcttgcaact gttcccggcg
    10561 acacggcaaa gcagcagccg gtatatgctc cggcaaatgt attgctaaag atgtttaccg
    10621 aagattatca gaagcaatac attaaatcgg cagaaagaaa agaacccggt aaggttcaga
    10681 tcattttcaa tatgccaaac cgtacgatac ccgaaatttc tttgcccgat gacgtggaat
    10741 tcctgatttc tacttcggca actaacgata cacttaccct atggttaacc tatgaaaaca
    10801 tgtccctgca tgatagcctg aatatatatt gctcctacta cgataaaacc accgatggag
    10861 ttcttgttta cgatacaata cgcattacca agcccttgaa aaatgaaaat tcattgctac
    10921 agttttccgg atccaggcat aaggagctgt attccgatta tctgttgaac ttttcgcatc
    10981 cgatagtttc gttcgattcc tcaaaaatag tattacagaa cgtagaatca cctgaaaaac
    11041 gaattgactt tacaatagca tcagccatta aaccggagca aatgctgtta aaagcaaacc
    11101 ttgccgaagg agaagaatat gtgctgcatt gtgacgaagg agcattctcc gattgttatg
    11161 gtggcagcac gaaatccgat tcaattcgtt tcaccgtaaa aaccgaagcc gattatggca
    11221 gcctgatcat tagtattccc accgcccctg aaacatgctt tgctgaattg ctggttcaaa
    11281 acaaagtaaa atattctgtg cgtattgccg acaacaggct ggctttcatg aacatttctc
    11341 ccgggaaata caccatcaga attattacgg acagtaattc aaacggagtc tgggataccg
    11401 gcaatctgct actgaaccgg cagccagaag ctgtgtatta ctacaacagc gaatatgagg
    11461 tgcgaagcaa gtggcaacat gaactggaat ggcagcagaa atttaacgaa caggggcaat
    11521 gaaaaaagtt ctgatcatca gctactattg gcccccggca ggcggtatcg gagtgcaccg
    11581 atgtctgaaa tttgccaaat atcttccgct gtttggttgg gagcccgttg tgtatgctcc
    11641 atcgaatgca cggtatgaat acaccgacac aactaatttc aggcatataa acgctgatct
    11701 tacagtaatt aaagccccta ttgttgaacc attcggtgca tttcgttttt taagcggacg
    11761 aaaaaaagct gaaacaacaa accctgtgta tgtgcgcgat cgaaagcgga gcctggtcga
    11821 taaggcagga atatggatac ggggtaattt atttgttccc gatgcacgca tgctttggat
    11881 acgcccctcc gtgaaacgac taaaaaaata ctgtcgcgaa aatcagatcg atgcattgct
    11941 taccgacggt ccgcctcaca ccaatacggt gattgcactt aggctttcgc agactcttgg
    12001 aattccgtgg cttgccgatt ttcaggatcc ctggacacag gtcgattatt atagcatgct
    12061 ctatattggc aagtgtgctg accgcaaata ccatcgctta gagcaggaaa gctttgccac
    12121 agcaaaaaaa atcaccattg ccagccctac ctgggcacgc gagctcgaaa gtatcggggc
    12181 aaaacaagtt gatccaattt tctggggtta cgacgatgac gatttccctg aggcactgcc
    12241 tccgtacagc gaatatttca gcattgttca cgccggacaa ctgggatacg accgatttcc
    12301 tgcaactttt ctgaaagtac tggcagactt atgtaacgaa aacccacagt tccgtgataa
    12361 aatgcgtctc gaacttgccg gaacggtaga ttacagcatt gcacaacgca taggggagct
    12421 tggactcaac agcaactaca agccattggg taaaatgagc ttccctgatg cggtaaaact
    12481 taccctgagt gctcatttac tcttgcttcc gctcaacata gccgacaatg ccaaaggaag
    12541 gattcccgga aaacttttcg aaaacatcaa ggccaggcgc ccgatacttt gccttggtcc
    12601 gtcaggtagc gacagcggaa acattattgc agaaaccctg tcgggcgaaa gctttgaata
    12661 tgataacgaa gaaggtatcc gtaactacat tatccgaatt ttcgagaaat atcagaacaa
    12721 ctgcaatgtg cttccaccat acgatataac agcgtattcg gtgaaaaatc aggtaaagcg
    12781 ccttgccaca tatctcgacc aaatcagtaa cagtaacatt acaaaaccat gaaatcgtac
    12841 cgccccgcat ttgctattgt caccgtatta ttttttatgt ggggcttcat tacctgcatg
    12901 aacgatattc tcattccatt tgttaaagca gtgttcgagc ttacccgcgc tcaaagcatg
    12961 ttgattcaga tggcattttt cagtgcttat tttttcggtt cgctgattta cttcataata
    13021 tcaactacga ccggagatcc catacagaaa atcggttata aaaacggcat agttgcaggg
    13081 ctaataatat cggcaattgg tacggggatg ctatatcctg ctgcagggct gagggtttac
    13141 ggattgtttc ttgcagcact ttttattttg gggttggggt ttacattact acagattgca
    13201 gcaaacccgt atgttgccat tctgggtgaa gaaaaaaccg cttcgggcag gcttaacctg
    13261 tcgcaggggt tcaattcctt cggtaccacc atagctccga tcatcggtgg atatctggtg
    13321 tttcatctgt tttcggggaa agggctttcg ctactcaatg cacagggaaa cccaataaca
    13381 cttgccgatg gcagcacagt ttcggcaatg ggagtgcaga taccttacct gatttttaca
    13441 ttcctgtttg ttgtgcttgc attggtcttt atttttatta aactccctca attctccgac
    13501 gaaacccatg ttgaaagagg gcttggagca ttgcgctacc ggcacctttt attcgggatg
    13561 tttgcaattt tcttttatgt gggaggcgaa gtgagcatag gaagcatcat gattaattac
    13621 cttcacgaac tgaaaggctt tgccgagatg gatgcgaaat catatctggc attgtactgg
    13681 ggcggactaa tgattgggcg gtttatcggt gcttatgcct ttggacaaca aaaaatatcg
    13741 gcaatcactt tccttggtat gttgggcatt tcgatcctgg ggttcggagt gatatacaca
    13801 gcaatttatc ttgaaggtgg caaaagcctg caccttttcc ttccgtatat tgcctatctg
    13861 gctctcaaca ttcttgcgtt ttatttcgga cgaaccagac caggacttac cctcagcatt
    13921 ttcgccggta tagctctttt attgatagga accagtatgt ttacccatac cgatctggca
    13981 atgtggagca ttttgggcat cgggctgttt aattcaataa tgtggtcaaa tatttttaca
    14041 ctagccatta aaggattagg taaatatacc agtcagggct cttcgcttct ggtaatgatg
    14101 attttgggag gtgcgcttct gccttttata accgggcatg ttgctgatat actgggagga
    14161 tatcagaaag ccttgttcgt tccagccatt tcgtacctct acctgatgtg gtacggatta
    14221 aaagggagta agcaaaaaaa cacaatacag gatcagagca agtattctgc gtgaacagca
    14281 aaagacacat agcgagaata ctatttataa gtatatcttt catactactg cactacagtg
    14341 cttttccaca aatgctctat ttacattaca gcgatagtgt taagcaggtt tgcggcgatt
    14401 cggttctcag taattatctg gtagatgaaa aacttcgcga atacgcaagt aatctagaca
    14461 gtgtttcttt ttttattatt gatgaaaacg aatgctttat cacatcggta aaggggattc
    14521 cgctctgtac gtcatgcggt attgctgcaa acagggtaat gcagacagcc tatcggctgg
    14581 ccttcatcga gaacttcatt attcagggat cctgctggga ctttgtcaac gctgttttta
    14641 caggtattac ttcagattca attcagaaaa aagatattta ttccgaaaaa aaagcaggcc
    14701 cttataccac tgtagatgag ttgcaacccg gtgattgggt gtatcatgtc aattatgagt
    14761 ataaccgtat tgaacacagt gctattttta taggctgggc cgattacgaa aaaggcttgg
    14821 caattgtgct cgactatgtg ggattgaaca ggcggcaacc ggcaaaattc ggtgaacata
    14881 acctgaattc ggtgtacgct atttttagaa tggaaaaagt tgattagttt ttattgaata
    14941 tgttgaaata taagggaata gtttccatgt gcctcatctt gtctgcagca atttcctgca
    15001 actggggcat ggataagatt aaatcgatca aagaaaccaa taaagcggat acttccgcaa
    15061 atggcagcct gattccggaa tttatccctg attcgagcct gaattttaac ttctgggctg
    15121 aagtaaccgg agcaggtgat acagtcatcg ccgattcatt gtttaaaggg ttcaatatgg
    15181 attattccga tgatactgtc agcatttttt gttttagagg aagttgccga cgcaatgccc
    15241 catcgagggg aacggttacg ggaaaaccca ccggattgga gcttgcatgg gtttttacaa
    15301 cagaatcaga tactaccaaa accaagtatg gtgtatgggg tggtggagca ggatggactg
    15361 ggcagcctct gttgttatgc tggacaaatg aacaaaagca aaaactggga ataaacgact
    15421 cccaatttct aaacaacgac aatgcttttg agctgatcat cggatcactt tgtggaaata
    15481 tatacttctt aaatgcatcc accggcagac ctacccgtga acatctctcc atcggaagcc
    15541 ctattaaagg tactgtctcg gttgacccca gaaaaaacgg attactttat gtggggcagg
    15601 gaatacaaac cggtgaaaaa tttggtgcat acgtgtttga tatgttcagc gggaaagaaa
    15661 tttatcatat cccgggtaat gacacatatt cttaccgcga ttggggggcc ttcgacagca
    15721 atccattggt cgataaagag agcggaacgg ttttctggcc tgccgaaaat ggggtaattt
    15781 atcgtttcgt tgctgatagc ctcaaacaaa taaaacaact cacaaggttt cactatcgcc
    15841 acgaccggat attccggcaa ggaattgagt cgagcatggc tgttattggt aactacggtt
    15901 tcttggctga taacagcggc acattgcttt gcctgaacct actcacaatg aagcctgtat
    15961 ggaatatcga taattttgac gacaccgacg cctccctgct tattgacgaa gagagcccgg
    16021 ggcaatatta tttgtacaca ggccatgaaa tcgataaact tgctccggtg agtgcctcgt
    16081 atttcagaaa aatcgatgca aaaaccggac acgaaatatg gcgaatcagc cgcgaatgcc
    16141 acggagacga cctcaacgga caggtcaaca gcggcggaat gctttcgagt ccagcattgg
    16201 gaaagcacaa gggaaacaat ctggtgtatt gcctgtattc aagaacagat tcgctcaatc
    16261 gttcggaatt ggttgctgta aataaaacaa cggggcagga agttttctcg gtacaattag
    16321 acaactactc atggtcgtcg ccggttgatt tctatgatac cgaaggcaat atttacctgt
    16381 ttttcgccga cgttaagggt aaattctata ttatcgacgg acttaccgga gaattgcttg
    16441 taactaaaac cacccaattc tcatttgaag cttctcctgt aatattaaac aataaaatat
    16501 acattgcctc ccgcggaaag agtattttgt gcttcataat caaaagccta taaatccggg
    16561 gattttatct taagcatttt tcccatttgt accatgttat taaatttatt atatttactg
    16621 aaaatattta acccataatt tcatgaaaaa tttactcttc gccattttta tgagtattac
    16681 cttaatttca caggctcaaa ctccggtagc acctgcaggt actgggacag aaaacgatcc
    16741 ttatcagatt acaacgctga acaacctcta ctggatgtcg gtaaccccaa catcatggga
    16801 taaatatttt gagcagactg ataatataga tgcaacttca acagctacct ggaattcagg
    16861 agcaggatgg gatccgattg gctatgttaa cggcgaatcg gattatttgg catttacagg
    16921 ctattataat gggaataact ggttaatagg agatttatat ataaatcgcc caactgaatt
    16981 agctgttggt tttttctcta ttatcgaaaa cgctcacatt tcacatatgg gcatttataa
    17041 tgcatcaaca gacataacag gaggggaagt gactggcatg ctggcaggct ttaatctgtc
    17101 atcaaccata attgattgtt ttggtgtggg gcatatttat ggggttagtg atctcggggg
    17161 attgattggc agaaatgaaa ccgggacaga atatctgtat gattgtagct ttgatggtgt
    17221 tgttgtcgga accggagata atatcggggg gctaattggt aaaaataaag gaactgttag
    17281 aaactgtaat acatttgcaa gtataaccgg aacctctaat ttaggcgcat taattggtga
    17341 aaataacgga gattttgccg ttgtggacag ctgcagagcc ggtggagcaa tttacgttct
    17401 tggaaacgaa ccaacggtaa acgccggtgg tttaatcgga gaaaataagg aaaatgctgt
    17461 tgttagctac tgctttagcc atacacaaat tcagggtacc gaaaatagtg aaaatttggg
    17521 aggcctagta ggattgaacg ataacggatc cataatcaaa tattgtttct ctacatattt
    17581 tgtttcagga ggtggtagtc ttggggggct ggttggttca aacagctatt ctacaataag
    17641 caattgttat tcgaggagta atgttaccgg aacacagaac aatgttggag gagtggttgg
    17701 tcgcaatacg gggactgttg aaaaaagtta tggaaccgga gttgtcacca gctcaggggg
    17761 ttgttttgga ggagtcgctg gaagtaacga agacggtgga gaagtttttg catgtttctg
    17821 ggatacgcaa acatcgggcg tggcagaagg gtgcggctac aatggcggag gtatgttaga
    17881 tatagttgga aaaactacag ttgaaatgaa aacccagacc actttcacaa atgcagggtg
    17941 ggatttttat ggagaatcag ccaacggaac cgaagataac tggtttataa cccagtatac
    18001 aaatgatagc tatccttgtt ttaaatggta cactgatttt gtaggtattc gcgaaaccag
    18061 caatgataaa aatacctttt ctgtttttcc caaccctgct agcgatatca ttttcgttac
    18121 agttttttcc ggtgaaacag aaattactaa gctatctatt tataccatca cgggtagttt
    18181 gctctattcg gctcctcatt atcaaagcaa cgagcctata gatatcagca acctaaaaac
    18241 cggtagttat tttattgaat taaaatctgc cgaatcaaaa tcaacaatta agctgattgt
    18301 caacaggtaa taaatcatca cctatgacaa ttgggtatta ttaaaaacct ctattagctt
    18361 atattgagca aataaatgca attttaaagt gtaataattc ggtcaaattt ttctgatttg
    18421 gattataatt attttcaata cttttgaatt gctaacttca gttttacaat gcgttttcgc
    18481 ggactcgcat atttacccgc aatcttgttc tggtggctga tatgctttca gctctaccga
    18541 ctggtgttct ttgtaatcat cacttcttgc tcgggaaatt ttgctgaagg catttaccat
    18601 ggatttgtca ccgacttagc agcagcggca tatctgatgg ttttgcccgg aacactgtgg
    18661 tttatttcaa actttatcaa gacacaaaac aggggctttt ttaaaattat tcttatcact
    18721 caaattgttc tcgctctatt tgtagctttt atcgaagttg taagcctgcc cttatacccc
    18781 gaatggggaa caacattcaa cttacgtgcg ctggtaagtg ccgaatcgcc gggagaagcc
    18841 tttactactt ttgtggattt tttaagctgg aaatggcttt tacttttttt tgctgaagca
    18901 ggtgtggcta taaccggttt ttattttata cagaattatt tttattaccg attggcaaat
    18961 aaatctgtaa gaattattta tcccattata attgccctat tactagtttt aggtattcgt
    19021 gggggcgtcg ggaaaaaact cattactgta ggcagtgttt tctataccca tcatcaatcg
    19081 gataactatt tggctgtgaa caaaacattt tattttattt cgacggtcaa gcagaaaaaa
    19141 caccttgata tcattaaaac agatattcag aaagaacaat taaatgctac ctatagaaag
    19201 ctgtacattg aagggaaagc atctgatacg ctcagggttt tcgacacccc ccgacccaac
    19261 attgtgctta tcgtactcga aggttgtccc gccgaagtat tttcaccact tggtggaagc
    19321 ccgggaattg ctccctgttt cgaaaaactt agttccgaag gactgctttt cacgcagttt
    19381 tatgcatcgg ggtttcgtac cgaccagggt ttgttgagta ttttaagcgg attgccggcg
    19441 ctaccgtatc tgaatataat gactgatcca aaaattgctg aagcactgcc atcgctaatg
    19501 caggtattgg gagagaaagg ttataatacg gcctttattt atggcgggga tgccagcttc
    19561 tcaaaaatta gtgattatat gaaaagcaac aacactgcca caatcatcga caaggcttcg
    19621 ttcaacaaac atcaatgccg gattgagtgg ggtgctcccg atgacgagtt gtttattatg
    19681 gccgggcaac aaataaaaaa atatagcgaa ccgttttttg catgtattct tacgcaaagc
    19741 acccatactc cattcgaaat agaaggccag cacaaattcc caggtgaaaa tatcccggaa
    19801 aagtataaaa gcagtgtgta ttttaccgac tcatgcattg gctcttttat tgaaagaagc
    19861 aggcaaacat catggttcaa caacaccata tttatcatca cagccgatca cgggagttta
    19921 tatcttggaa ctcatgactt caacgatcat gaacgtttca ggattccgtt gctgattttt
    19981 gggactcccc ttgccgagca atatagggga aaaactattt cacgggttgg agcaagcttc
    20041 gatattccgg ccacaatact tgcccaactc aatattgaaa accatgattt tcctttcagc
    20101 aaaaatttgt tccacaaaaa aatgtttgac catgcatttt gggcaaccga tcaaatcatg
    20161 ggttggatta caaatgataa aaaaatcgtt ttcaattttt cgaatagcga catttacagt
    20221 accgaaggtg ataccgttca taatgcagag agtgtagagg atgcaatcag gttttacaaa
    20281 ctagtggcag aatatgcctt gctcaaaaaa actccatcgg tacgctgaac aaaaaggcat
    20341 ttcaatctgt tgacattttg ccatctggta aaaatgaaac atattttgaa aaaagttgtt
    20401 tcattcatta cttttgacgc aaaaaacgat agtcatgaat gacaaaaacc taattgccgg
    20461 aatcgacatc ggtggaaccg gcactaaaat cgggattgtg gatttcgatg gtaaaataat
    20521 tgctcaggca cttatcgaca cccgggagtt ttccaccatg gaattgtttg tatcggcatg
    20581 catcaaaaaa ataagcagcc ttagtaattt acacagactg gcaggaattg gcattggggc
    20641 acccaatggc aattactata gcggatgtgt tgaaaatgcc ccaaatctgc catggagggg
    20701 taaactggaa ttggcgggtg agttcacaaa acaatcagga ttaaaagcca ttctcaccaa
    20761 cgatgccaat gcagcagcac tgggtgaaat gatgttcgga ggagctaaag gtatcgggca
    20821 ttttttctac ataactctcg gtacgggtgt aggcagcggg attgttgccg atggaaaatt
    20881 aatttacgga cacgacggaa tggcagggga actgggacat accattgctg taaaaggagg
    20941 aagaatgtgc ggatgcggca gaaagggatg tcttgagatg tacgcttctg taagaggact
    21001 ggtattaacc gcgcgggaac taatttcgca atatcccgga cagtcagcgc ttgaatcaaa
    21061 gattgatttt tcggcaaaag atatatcgga tgctgcaaaa aacggagatg ctattgcact
    21121 taaaaccttt gatatcactg ccgaaatact tgcgatttcg cttgccaacg cctctgcagt
    21181 aacatcccct tcgcatattt tcatttttgg gggtcttgca aagtcgggag aattattaat
    21241 ggtacccctc aaaaaatact acgaacagag tcttctgaac gtttatcaaa ataaagtatg
    21301 cctttctgtt tcggaattgc ccgattcaga tgctgcaata cttggggcgg cagcactaat
    21361 cagaaattac tttatgtaag cccaaactct aaggtaaaaa taaagttgaa tgtcttaatt
    21421 tactatattt gcagattaat aaaatactat gattaccacc cgtttaaccg aagacgaaac
    21481 gctgttgcag tcgataaata tggctattct cgataaaaaa ggcgaggata taattaatgt
    21541 tgatttgagg aacaccacgg gctctgttac aaattatttt gtgatatgta atgcaaattc
    21601 tattactcag gtacaagcta ttgcagagca cgttgaaaga aaagtgagga ctgagttaaa
    21661 agaaaaagca tggcatagcg aaggttacga aaactgtgtg tgggtattac tcgattatgt
    21721 aagcgtggtg gttcatatat ttgaaaagaa aaatcgtgag ttttacaaac tggaaaattt
    21781 atgggctgat gccgtttttt cacacatcgg taatgatggt aaaattgcgc tggttccgta
    21841 atattttaat agttattctc tgaattaaat tacttagaaa taatatgtcg gaaaacagaa
    21901 aaggatttga tcctcaaaaa tctgaaacac ccccagggga aaagcaaaaa cccaaaatta
    21961 gcgggtattg gatttatatt gttattacca ttctgctcat agtatttact ttttgggatt
    22021 ttggtccctc ggcaaaggaa accaacagaa atgtattcga aaagcagatg cttgccaata
    22081 atgatgttgc caaaattgta attatcaaca aagaaaaagt tgaggtttat attaaaccgg
    22141 acaaactcag tcaggaaaag tataaggaag tttcgggaag tggaatgggc tcaccgggga
    22201 aaaaggggcc acattattat tttatgattg gtgatgttga aacatttgaa aggcagcttg
    22261 cagaggttca ggcatcgctc ccacccgaaa ataaaatcac tgctgagtat agaactgaaa
    22321 gaaactattt tggtgaaatt ttgggctggc tgctgcccat aataattctg gtggctctgt
    22381 gggtatttct tttccgtaga atgagcggag gtggtggttc tggaggaaat cagatattta
    22441 atatcggcaa gtcaaaagca caattgttcg ataaagaaaa gcacgttaac accaacttca
    22501 acgacgtagc cggtcttgat gaggctaaag ttgaaattat ggaaattgtt gactttctga
    22561 aaaacccgaa acgatatacc gaactgggcg gaaaaattcc caagggggca ttattggtgg
    22621 gccctccggg aactgggaaa acccttttgg caaaagctgt tgctggtgaa gccaatgttc
    22681 cctttttctc tatgtcgggt tccgatttcg tagaaatgtt tgtcggtgta ggagcatcaa
    22741 gggttcgcga tttattcaaa caagccaagc aaaaggcccc atgcatagtg tttatcgatg
    22801 aaattgatgc catcgggcgc gcccggggga gaaatccgaa ctttaattct aacgatgaac
    22861 gcgaaaacac cctgaatcag ttgcttaccg aaatggacgg attcgcatca aacagcggcg
    22921 tcattattct ggctgcaacc aatcgcgccg atgtgcttga taaagcactg ttgcgtgccg
    22981 gaagattcga cagacaaata catgttgaat tgccagattt gcatgaaaga gaggctattt
    23041 ttaaagttca tgtaaggcca atcaaactcg acaaagatgt tttgcttgat tttctggcca
    23101 aacagacacc cggattttcg ggtgccgata tagccaatgt ttgtaatgaa gcagccctta
    23161 ttgctgcaag gaaaaacaat aaaaccgtga ccaggcagga ttttctcgat gcagttgacc
    23221 ggattatcgg tggattggaa aagaaaaaca agattatcag tgtgaacgaa aagaaaacca
    23281 ttgcttatca cgaagcaggt catgccagtg tgggctggtt gctggaacat gcgcacccgt
    23341 tactcaaagt tacaataatt cccaggggaa aagcacttgg agctgcatgg tacctgccgg
    23401 aagagcgaca aataaccact acggagcaaa tgctcgatga aatatgctct acacttggcg
    23461 gcagggctgc cgaagaagta tttttcggta aagtttcaac tggagctttg aacgaccttg
    23521 aaagggttac aaaacaagca tttgcaatgg tggcttattt cggcatgaac gataaaatcg
    23581 gaaatctttc gtattacgac tctaccggac aaaccgaatt cagtttcagt aaaccattca
    23641 gcgaaaaaac ggcagaatct atcgatgagg aagtgaagct aattgttgaa caacaatatg
    23701 aaagagcaaa agaattgctt accttacata aggagggcat tgaaaaactt gcggaaaagc
    23761 tgcttgagaa ggaagtgatt ttcggcgatg accttgaggc cattttcggt aagaaaagag
    23821 gtaaagaaag agagattgaa gaaaaaccag atgttcagac ctccggatct cctattgatg
    23881 ctttgcagtc tgaatcgggt caggagggta aactttccga aactgataca agcaagcatg
    23941 acgacacaga agaactgggt taaagtgtac tccaccgggg tggagtacct tgccgaattg
    24001 cttaagcagg tgttgttcga taatggcata gaagccgttg ttgtagataa gaaagacaat
    24061 gcgtatctgt ttgggtggat tgaggtgtat gtttcagatg atgattacga aaattccgtc
    24121 aaaatagttg aagattttga aaaaaatacc agcattaaat agtatgcccc aacgcgctct
    24181 cagtggagca gtgtatgtag gcatggtggt gttttgcctt tggttttcgg agtattctat
    24241 gatttcgtta tttctgtttt tactgttttt actgcactat gaattcaaca cccttattaa
    24301 caggaaaaaa tacaagctac ccgcgatagt tacgagcgtt atatcaataa gcctgttgct
    24361 tgcaacattc atttacgaaa aaaccaatgg cagtactgaa ttgttctata ttccactgtt
    24421 attatttgtt ctattacctc tcttgtatat tcttatcgat aaagaacacg atttgcgcac
    24481 actgggggtt tttcttctgt cagttattta ttttgctgcg cccctatcgc ttgctgtatt
    24541 aattagtttc cggcataccg aagaaccggt agttacaact tcttatcagg cgtttccagt
    24601 tttatcgttg tttgtgtttt tatgggttta cgatacattt gcttacctcg ccggaacaat
    24661 atttggtaaa acaccactga ttgagcgaat ttcaccaaaa aaaacatggg aaggtgttat
    24721 tgggggcgtg gtaatgacct tacttctgtc gttggcttgt tctttaattt ttaatatttt
    24781 cccaatatca catgcagtag caatttcgct gattgtcgta attttcggta ctcttggcga
    24841 cttgtttgaa tcgtggatta aacgaagcgt tgcaattaag gatttcggga acattatgcc
    24901 cggtcacggt ggtgcgttag accgacttga ttcgttttta ttttcggtcc ctgcaatgtg
    24961 ggtattttac agtttattcg gctgaagatt atcgtaaaat atacatttta cccgtatcat
    25021 tcttaaagta attatcggca gccgactgtc ggtggtcaac agaatttccg ttaatccttg
    25081 cctttacacg gtagaaatat actccatttg caagcctgtc tccgaacata tcggttccat
    25141 cccaggcata ggtagttatg ttacggccaa tgtgcacacc cccaagctca aataaatcga
    25201 ttactttaac aagtttaccc gtaactgtaa atatttcgat ggtaaaatcg tcggggattt
    25261 ccgacccggt tagttcaaac acaaaccgcg ttgatgtact gaagggattg ggataattca
    25321 ataggttggt aatagctgat tcagtaacaa ttttaaaaga tattagatag tcgaaatcgc
    25381 ctgattcatt tcccgaaata tcggttgcct gaacgcgtaa ttgatatgtt ccatcgctgt
    25441 tgaaaaccgg tctgaaaatt attttacagc tgttgtcggg aagttgcgct ggcacccatt
    25501 ccatattgta ccaactgcta acagcattaa agaatattct tgtttccgtt ccgttttcat
    25561 tatcggttaa atacacgcga aacaacgatg tatcgttcaa cgctaaatat ttgttctcat
    25621 ccttaagagt tatcagaatt tcaggggtag ccgaaacaat atcgccatcc atgatatgta
    25681 taccatcgaa gctaacatca agaaggggat tggcttcatc gctgaggaca taaaagtact
    25741 tctgcgcaat attgttaaaa tgatgctgtt ctatctgatc gtaataaccg agtaggtcat
    25801 taatcggatt atattcaacc catacactgt tcaatccact cattccatgg gtttccaatc
    25861 taatggtatc aattattata tctccggcag gatgtggccg aagccgcctt gttgcaatgg
    25921 gaatgatctg gttattttta tcctgcagcc aaaattttac cagtaggcta tccatatcga
    25981 ccgtgctcac atttcgcgtt gcgatggcaa aaacaatctc gtcgccttcg ttaatcgtgt
    26041 cgcagcagaa atgataacca tcacgcacat ttattgcagt ttcgggagcc tcatcaaaat
    26101 ataattgcca cttatcgagt tgcgccggag ttttatccaa atcatcccga ctgtagaatt
    26161 tcatctgaag atatgggtat tcggtggcag caatcgtatt gctaatgtca gggataagat
    26221 aattatccgg tttaatacta tctataagaa tatcaaccgt accatcgctt ctaatccctt
    26281 caatacaaac aatggcagta tcatagcttg gcgattcaag gctgtgttga agccactcaa
    26341 agcttgtcca cgctgacgcc ggaccgatag ttttcgataa aatataacca taattgaaat
    26401 cggtaaataa atcggcctgc aatgtgcaat acgggttttg ggccgtactg tacgattctt
    26461 ttgcagatga agggcttcct ttgcggcaga agaaaatata tgcctgatcg ttggtaaggt
    26521 cgcgtatttg ggttgaacca agggcttcaa aattacttag aacattttct tcccattgtt
    26581 cgaaatacgc atttgtccat gaataaaaca gtacataatg cccgttggga atactgagaa
    26641 tcatgttgtt catattctgg attccttcgg aataatactc tgttccccag ccagagctga
    26701 aaataaaata ataatttacc cttccactgg aaccgcattg cggataattg cgatgtccgt
    26761 aattggaata atccgactga tatgccagta gtgtaaccgg atcaattaca gccactatta
    26821 ctgcgggttg atttccacag catccgtgat caccgaggcc attgttaagc cctccatcga
    26881 tagtaaatct cacatcggag taggtttgtg gactaacata acgctggtta taaaccatca
    26941 ggctgcgtgg tacggtaaca aaatcgaagg ccctgttaac cctgttataa tcgataaatt
    27001 gatatcggtc gtttttaaac tgaaagaaat gcgcctgact ccagccttct ttatgcggta
    27061 tataaataaa ggagctttct ttccagataa tgctgtcggt aattgtatga tttcttgcaa
    27121 tgcgccagta gtaaacggtg ctgtcgttca gaaccatggg tacgtcccaa tataacaagc
    27181 ctcccgagga actcagtgtt tgctgcaata aaggcaaccc aaccaagcta ttaaaggtgt
    27241 cggaagtgtc gatctgaaat atatactgag catctccaag aaatgggtct ccggtagaag
    27301 caatcaggcg tacgtggtct tcggggatta ttgcgaattc atagggaaaa accggaaaaa
    27361 gatcgcttga ccttattaga aagctaatac ttgttaggtt attgttttcg ttgaattctt
    27421 ctacagtatt cattgcatcg gtaaaaacat caagtttgtt taatcccggc cctataattt
    27481 tattcaccgg aagccggatg tttatcgtat ccctatataa acatccatca actccgagta
    27541 catattcagt catagttcca tcgggatatg tacggttgac attcactgta aatccggaag
    27601 atgtcgtgcg cccaatattg gaaacgacta tctgaacatc gaaactgtcg atagtggtat
    27661 taatttcgtt gggtaaaaat gcaatatcgg agttttttac agtaaggtcg ggcaattcga
    27721 atgcgtttat cgcaattata gggtcaccat gtagtgtgaa ttcgtggcag gtaatttcca
    27781 ttctcacgtt tgtgagaaat acgacctctg ctgcctgaat ggcttttatc agctgcaaac
    27841 caatcgggct tccgtaataa tcgcgtgaga ccttattgta taattctgta gaataaaaat
    27901 taagatacga tgggaggcct tcgccaactg acgcaagaaa tgcaatagcg cctttatcct
    27961 gtatcaatac ccattcctcg cttgtgcagg tgttactgta taagtgaata tctcctgcat
    28021 aacaactgtt ggctaaaaca aacgggtact taccctgatt ctcgtagttt gccggatcgt
    28081 cgatactttg atcaaaacca ttcgctgaag catgcccaaa aaaagtcatc aggctcactc
    28141 cattgtttat cagatttcgt actgaatcgc tttgggttat ctgaaccggg gcagagctgt
    28201 tctttaaaaa agtacttaca tatccgccga atagagagtc ctcgataatt gcctcataat
    28261 tgcgtaaaaa tcccgcaaaa taggtctgtt ccgactcatt ggctcctcct ccgaaatgta
    28321 ggacattctt catccattcg gcaggttcat tgttttcgta ttgtattact ttattcaaat
    28381 agatcaatgc ttcttcgttg gttctggcac atattctacc ggttgccaac agtggttcat
    28441 agcccgtacc gtcgaaaggt gctgtgaata aattgtcgct tgatggatat cccatgctgg
    28501 gaaccaaaca ataagcatac gcattggggt cgttacgggt atcggggcaa tggaccgatt
    28561 tcccaaacaa aaaaagatat tcaggttgta ctgtgttatt gttcaccaga taaaaaacaa
    28621 aatttctgat tccaatcggg tgtttctgaa ccccgtatgc aaattgattg tacagctggt
    28681 caacgtctgc tatcagtaca ttctcaccgc gtccggaccg ataggccgca tattgttgcg
    28741 cagagcccat caacgattta tgagtgatta taagataggt cgcctggggg tataacgcgt
    28801 ggtaattgat aaatttatta ttatcagaca ctttgtaaat accatcaact tgttttaatg
    28861 agttatcgcc gcacataata cagcgccttt cagagccatt ttcgggaatg agagctttat
    28921 gaactgttcc ctgttttact acttttattc gccgatgaga atccaaatca tagaaaacaa
    28981 cgtcgtttcc gtcggttgaa ataaaattat ttaactgaag gagttgtttc cctccacttc
    29041 cggcaggcac tgtaaattcg aaataggatt ggttcccggc attgagtgtg cgcggatagg
    29101 taagctgaat gtaagaaaca accatctgat cgggaaccga agttagggta acatcgttag
    29161 ccgaaaaatt caggttcaaa cctgttgata agcttgaaag agctattgaa gccgttccaa
    29221 ctgcataatc ataacccaga aattgggttt cgataacggg tgagtcgttt aactttacaa
    29281 ttaaatgatg aggaaaaaaa ttctcaggag cacttgcagc aaaacccgat actgcagttt
    29341 cgagggtagc gctgggcccg gatgcataga tatagggaac ggttaatgac ttatttaccg
    29401 tggcatcacc atcggggtga tctttacgca atatttcatt gtcgaaccaa ccctctccat
    29461 caccaaaaat acagcgaatt gagcctttta caaatgtgga aacataattt tgccggataa
    29521 tacaccagag gtatggaatc aggttcgaag tgtattgggt aaaatcggta tcatcttcaa
    29581 cgatcattct acggttattc gttgtgttgt tccatgttaa aaaataagat gcagtatcgt
    29641 taaaatagct ataatcatga tttacgatat cctccgaaaa ggtataggcc atagaatcaa
    29701 tacccgctct gttacgtttt ccgtaaaatt ctatataccc gcttggatcg aaaatacccg
    29761 atgtcccctc tcctttaatg tagatatatt gttcttctcc ttcatggaac aattgaatat
    29821 tcctggggtc aatctgatcg accggaatac cggcagatat cagctgatta taagagatct
    29881 tgtaaacacc atcggtgtga accgcaatgc ggaaatattg ctgatcgtaa tttatccatt
    29941 cgttgttgaa ttgctgcgaa aacaaaaagc tgcaatgcaa catcaaaaca gataatatga
    30001 caattttgat tttcaccgat ttaatttcca ctaagttata acattaaacc gttccattga
    30061 aattaaattc gataagctgg tcattgtttt ttaaggtcga accgatagct taacgaaaac
    30121 acatgagaat acagtgctat actttggttc ccgatatcgg ttagtgcata atcgattctg
    30181 aatcctttat aatggattcc aatgcccgca ttgggttgcc acgacatggt gctggtatca
    30241 tcgaaatcgg gcaggttttg aaaattggaa actcccagtc gtaaaaagac cagattgtta
    30301 tatgatgctt cgagaccaag gtgtgggtca acgctgaaaa catcggtttt tataagtgta
    30361 tttcttttcc cgtcgaatgt tacgtctaat ccgagttccg ggtaaatcag gaatttgttt
    30421 ttaatataaa atttataccc tgcaccaagt aataatcgtg gaagagtaat ttcaagccca
    30481 ttttcgggta tttcgtttcc ggtttgttca aacacttctt caaaaatttc gcggttgtaa
    30541 ctccacgcat taaaagtgga agttatatcg cgggcctgtg caccaaaaag ccagttcttc
    30601 atggcatact gtgccccgat atcaaaacca aaaccccatg caccgccaaa atcaccggca
    30661 attctgcgga ttaccttaaa attggctcca taattcaatc cttgcacagg agatgttctg
    30721 gagtagctta acaagaaagc ataatcagct accgaaaacg atgaaatcct gtcgtagcgt
    30781 atattaccgt ctccgtcaat cagatcgagg gtattgggaa tatcatcgac accaaaacgt
    30841 atcatcgata ctcccagggc actattatca tcaattttat aggctcctcc cagaaaatcg
    30901 tatttcgata atccggcaaa atattctgag tgcatggcac caatttcgat ttttctatcg
    30961 agtgatacca atccggccgg attccagtag agagaagcaa catcgcccac tgaggctatt
    31021 accgagtttc ccatagctaa ggacctcgct cccacaccga ttgacaaaaa ttcgttgcta
    31081 tatttggggg ctgtctgaga aaaaacaaca ttgcttaaaa cagccaacag aattactaat
    31141 tttgtacgca aaagcatgaa cttataattt atgtgtaaaa ttaattattt tatattaaac
    31201 ttcgtaaact tcaatttatt gcttaccgaa aagtaaagat aatgaaaacc ttaaaaagtc
    31261 tgataccaaa tctaataacc agtttaaacc tctgttttgg tgcggcagca attgttttcg
    31321 cttttgcacg tcagcccgaa acagcgtttt ggtgcattat tattgctgcc ggttgtgatt
    31381 ttgctgatgg cttctccgca agactattga atgcttatag cgaatttggc aagcagcttg
    31441 attcgttgag tgacctgata agtttcggtg tagcaccggc agcacttgtt tttgtatatt
    31501 tcaccgatac cgctgataat gggtttccga gctggattgc attcaatgct tttacggtgg
    31561 ctttgttttc ggcataccgg cttgcgcggt ttaatatttc accaccatca tcagatttcc
    31621 gtgggttggc agttcctgca agtgcattgt atctgataag cctgatatgt tttggggccg
    31681 gtttaccaaa accgatttct gaagttgtac tttctgctcc cgctgtgcat attcaggtgc
    31741 ttattattaa tctgctgatg atttcatcca ttcccatgtt ttcgctgaag tttaaaaatt
    31801 tcaattttac cgaaaacctg attcgtttta tatttctttc tgttggggta gttcttattg
    31861 ccattttcgg gatacccggg atatggttcg ttatggtttt ttatataatt ttgtcgttgg
    31921 caacagccat atatcattca aaaaaaactc aattatgaaa tttcttgcaa acatcgacgt
    31981 aatgcctctg aaagcactac tcgaccctca gggcaaagca gttaacaaca gcctgaaaaa
    32041 aaccggtttc gaaagcattg aaaacgtaag gataggcaaa catatttctt tcgaaattga
    32101 agccactgac cagaaaagtg ctgaaaaaac ggttgatgaa gtttgtcgca aagtacttac
    32161 caaccccatt atggaagggt tcacatttgt tgttgttaaa acagaataga atatttgaaa
    32221 ataccacaaa ttcgttaagg actgaaatta tttgattagt atttgcagaa tctaaaaaaa
    32281 atataacttt gcagtcctca tacggtggtt gtagctcagt tggttagagc gccagattgt
    32341 ggctctggag gtcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntacgg
    32461 tggttgtagc tcagttggtt agagcgccag attgtggctc tggaggtcgc gagttcgaat
    32521 ctcgtcttcc acccaaaaac gttttcgaaa gaaggcgttt ttttataggt tttatccgag
    32581 tagttcacgt tttattttcg acagtaattt tttggtgcgg tacctggtat agttaatctg
    32641 atgtcccctg tttatttcca cttcattgaa ggctttccag ctaaggtcga ataaatcgcg
    32701 atacacatgg ttcttggtat gttgcttatg tcggcgatgt atcgacatgg caataagcca
    32761 aattattcgg gaattccatg gctcaaaaac ctcttgcacc aaatcccaat ccgaccggtt
    32821 tgccgaattc catcgcccca tgcgttgctc catgaagaat aagtcttcca cctgcaattt
    32881 caaatcttta taaacctgcc gtggttccac aatccacttt ttaaattcct ctccggcaaa
    32941 ctccaaatga ccatagtcgg taaaatttct tacatcgtgc aaatcggcat tatatggtac
    33001 ataagcatat cgttgacgtg caatctcatt cattacactt tgcaccgata agtattcctg
    33061 ggggtatcct gcaagtaggt tataggccat ttcgtaatac gtgggtttga agtttgcatt
    33121 gttgtttctg tatgcttctt caaagtcttt gtccacttct tctgtttgcc tgattaccca
    33181 atggggtatt ccaagatgtc ggcccaatgc agcaggaatg cgtatatctg gagactcatc
    33241 cgtcaatttc cggttctgaa tagtgtacat tataaattcc ttactgcagt tgtgcataaa
    33301 gtatcccacc gatatccggc tgtctgaccc cgaagtggtc ccaagcgcca gtaacgggta
    33361 acgtttctgt attgcattgg gggttttcga caatatttcg tgaagatttt gaatcaggtc
    33421 tttataattg agttccatgc gctcaatggg ccagaatctt gatacttttt tattttttat
    33481 atcgtatagc gtattggaaa taaggcattt aatgttttcg aaattggttg atgtattggg
    33541 aaaccaggtt gcatcggggt tgatgatttt ttgcacaaat tctttctgta taaatgccga
    33601 tttttgttct cccgttgcca ccgataacag catttcggtg gaagccagag taacgtcccc
    33661 accgataaaa tgaaaaatct gcctcatccc tgtagcatca ttgaacatta tttcatcgtt
    33721 gatatcagaa taatataaaa cccaccttcc ccctgcatga ttggtgtttt ttataaaact
    33781 gtctttaccg gtcgcattat caagatattt cccgattatt tcttcgttcg ataattccgg
    33841 atatcggtaa tcaataatat atccaagtaa aacagctttt ttgcctgctt tttcaataac
    33901 tgagtaattc agatcggggt gaacatataa aaaatattta ccggtcttaa ttatttccca
    33961 atgcgaaaat tgagacagct ctttgttgac aaaaataaac tgccttctga attttaaccg
    34021 atcttttaat tctgcattca tgtattgcaa cagtatatga gtttatttcc gtgttttcag
    34081 tttcgaaata taatctctgg ccatggcaac cggagcaatt ttcatattta ccaacatacg
    34141 atataataaa aacgtaaacc ctccgataat aaaaagctta ataaatataa gcaacacata
    34201 gttcaccggc aaataatgaa taaacgacaa tgagccgcaa accaaaacga ccaatattac
    34261 gtttaagcta tcgaatctat actctgtgct ccgcatggca atgataatct gtatcaagct
    34321 caacactaag aaccccatat tcctggctat taaagcccag taggcattaa aaaaacttgt
    34381 aaaagcaaaa cataatccca aattaacagc aaaaccagca aaattactta tgaaaaggta
    34441 tttagaattt ttcttaccgt ggtacacaaa cattgtatac acctggccaa tgaatattac
    34501 aagtaatcca attgttgaaa gtggaatata tttccatgcc tcaagtaatt cggggttgct
    34561 cgacagaaga cttaatattt cgtatccaaa tatagaaacc cctatgcaaa agaaacctgc
    34621 accccagaaa atgatattca acaattttcg gatataatcg tggttatttt ctttgaaatt
    34681 ttcgtagaac caaggggtaa aagcaaaatt gaacgactcc ttaaacatcg agaagatggt
    34741 cgaaaaagtt agcgcaatat aataaatgcc actaatttca acaccattcg tcctgttcag
    34801 ataaatggag tcgacactcg tgagtaaaaa acccgcaagg aaaaatggaa taagggggat
    34861 gctgaactta aacaccggtt tcatgaggcg aaaatcgaag cgcagcactg ttttacggta
    34921 atataccaga attgcgtaca gtgcaaaact aaaggagcaa atcagattgc tcagaattat
    34981 ccccagcgca tccattttaa aaacaaccac aaggagcagg ttcaaggcaa tatttgaacc
    35041 aaaaaacgac atatcgagct tgaatacttt cgctccaatg tgctttgcca tcaataaacc
    35101 tttatattgt tcgtacacaa tcataaatgg aactgtggcg agtgcatagg ccatataggg
    35161 gaaaaatcga atttcgttaa gcacagggct taacaatcga cttccgaaag gtattaataa
    35221 tgccacaagt atcaggttga acagtatggc aatgagaaaa ctcgaaccca taagacgctt
    35281 ttcgtaattt tcctgttcag catctttttc gtaaagatat cgggtaatcc ctttaggcaa
    35341 ctgaaggcat gaagtactta ttattatggt aacaatcatc tgggttattg ccataatgcc
    35401 gtaatcggct ttggtaaggt acgatgaaaa tattgggaag aatataatgg aggaggctct
    35461 taaaataatg cttgagaaag agtagaaaaa cgtattctta aaaacacttt ttaaattagc
    35521 cattctccgt attttttgca attgtagtct cttttaggca tatttcaaaa aaaattagta
    35581 gttcagaatt attctgtgat taatacctaa atttgtggtg caacaaaaaa aagcatgaag
    35641 cgggtttatc atcggggttt tttaatatta tcaaagctcc ttttatgggt aggatatgtg
    35701 tgtttaccgt ttattaaact aaaaaaaata aattcaagta attatgcagt gttgccttac
    35761 actcctgaaa actggcccgg tggaaaagac cgtatggcag catggaaacc ctattttgca
    35821 gaaatttcag ccgcatattc aattttctgg gcatggcagg aagatgaaat gcagataatt
    35881 ctggaatacg gtggatctga gaatgttgtt ccggcctata aagtgtacac taaaatactt
    35941 attcgcagat ttcgtttact attctcgtta agaaactata aatcggtgtg ggtgcaacgt
    36001 gcatttgttc catcatttcc tttcagaaat gcctacttcg agaggcttgc atcttttatt
    36061 catccataca tagtgtacga cttttacgat gccgattata ttcataataa aaatctggtg
    36121 gatcagacag caaggcttgc tttcagggtt tcggttgcca gcaagcatct ttacaattac
    36181 ttcagcaatc tgaatcctgc gactttcttt ttacgatatg cgataattgc agatcacttc
    36241 atcgagaagc caacaaacaa cgatgcgcaa actattcata tcggctggat gggaagtccc
    36301 gacaatgcaa aacaactgat tgagatatcg gcagcattat ccaagcttga ggaggagttt
    36361 gagcacattg ttttctcgtt tacatgccga aaattgccaa aacttccttt tgcgcgactc
    36421 gagagacatt cctggggcga cgagggattt tcgtataaca catggttggc cggattagac
    36481 atcggtattg ttccttatat gcatcctacc gaaaccgtga aagccaaaat agcaatgaaa
    36541 agtctggaat ttatgggcaa tgcagttccc atggttgttt ctccctacat gcactctgat
    36601 atgcttgttc atggcagaga tgtgtatatt gcctcaacaa cagacgattg gtacgaaatg
    36661 ctgaaagcct tgattatttc aaaggaaaaa aggactgcta tgggaaaagc agcaaaactt
    36721 atttttgata cttaccatag ttttcggaac aactttgaac agctgaaaca aattttagaa
    36781 ccctaagtta gaatacttgc gcattaaacc ttacgtttgc ttacattatg gcagatgtat
    36841 gcctagtttc ggattttttc acaaacaaaa atgtaataat aatattcagc caaccgcggc
    36901 ttcctgttaa tactcaattt ttcgagccag aaaaaacagg gtaatagcca gtttcgtgtt
    36961 aacttcaatt ttttccaata agcaatactg aatgaaggca tccgaataat cggcttcgac
    37021 agctttaccg aaaacccgct ctctgtgagc aagcgcatcc aagtgttcat gcttcgctgg
    37081 acccccactc cacctaaaat cctgtcggtg gggctaacgt gttcgataaa aacaagtctt
    37141 ccattttcag aaagtagcgc tccgaagttt tttaatcgca atatcaggtc ttcctctgaa
    37201 atgtgataca ttacacccac ggtaaatatt ttatcgacat gcgcctccaa ttcaatggga
    37261 gcgtacacaa aatcgatctt acgaaattca acattattgc aattattccg cctgctggca
    37321 atttctatca attcttcaga tggatccact ccaatcattc gcttacattt tgactgcagc
    37381 aggaaactta agcgccctat accgcatccg aaatcaagaa ttatatcgct tggttgaggg
    37441 ttcaaatacc acaacaaatt tctttttgta agatagtcga gcaatatgtc tgatctgagc
    37501 gatacttcat cgttgctgcc gataagaacc gaatggtctc ctgcgagcgt cgatcgtact
    37561 ttgtacatct tgctgacttt gttataattt atttcttcca tatgtttctg atgttattaa
    37621 atcctgaata tattttgtac ctggtggcat gtaacaccac ttcattttgc caagaaggct
    37681 cgatgctgca gccgaattta agtttaaaat cggaaactgt acccattttc atattatcgg
    37741 ggtcagcatc agccacctgg ccaaaacctg cagtttcgaa acccatggct ttatagcgca
    37801 tcatcatgtg ccaatgaatc atatgtccgc ttccaagcat atttcgatgc attccaccat
    37861 gcatgtattc cactcttttc ccgaatttaa ttaccatgat agctgagaga tactctcctt
    37921 tgtccttaac aaagcatatt tctgctgcat tatatttcag tgccgtggaa tacaaatcgt
    37981 acagatagcg cctatcggga ggtttaatac tttgcccggc ataggtttcg ataagtaagc
    38041 ggtagaaatc atcaaaatta tcctccacag caaactccag tccgtttttc tgtgctttca
    38101 gaatataccg tcggtgggtt ttataaattg catcccaaag tgtatcgaga ttattactaa
    38161 gcgaaagaca ggcaaatgat attggtttct ttttgttgat tgctgaatca aaaatgaggt
    38221 tataaccacc gtaaacaggt ttcgatttaa aataattata gccctcagtg tttaaatatt
    38281 tcaacacttc tatggctaaa agtcctaaaa cttcgtcatt attttcgata ttgcaattta
    38341 tgacgggttc accaaaggca tgtgcctgac ggctgataga cttcagaaat ggttttcctg
    38401 attgataggt atatatttta attcctgcca cgagcacctc gtttttgaag acttgaaaat
    38461 acaccggttt atttttaaaa cctgcattta cctcatcata ataggttgtc tggaaaaaat
    38521 tagagcatac attgcagata gcgttccatt tttctgactg caacggagcc atctcggtaa
    38581 tatgaatgga agtatctttc aactcagatg tattcaatgc gtaaaattag caaaatatac
    38641 accgcattcc gataatatca agccgaattg attaaatttg cttttatttc gttgatttat
    38701 gaagaaagta tgcgtacttc ttacaagttc aattcataaa gacttcaggg ttataaaaac
    38761 cattcaggta ttaagccggt actgtaaagt tgatttattt tatctgcgcc ccagcgacag
    38821 cgataaagag ctctttaacg aaaacacccg gctatttgct cttaaacaac ggactggaaa
    38881 gaaagaactg tttttaaggc atactttctt ttacaacgag tttaattatt tttttttcga
    38941 cataatccgt catgaaatca gatacgacta tgtttgggca aacgatttac ctgcacttaa
    39001 accagcttta tttctgaaaa agaaattcaa cgcaaaattg gtttacgatt ctcatgaaat
    39061 ctatattgaa acactcaatc agttttttcc tgaaaaatcg catggcataa aaaaaatcgt
    39121 tttcggtgcc ttactgaagc tgatgcgatt ctatggcagc agggccgaaa gggctatggt
    39181 ggagcaaacc gacatattta tcactgtaag cgattcggtg aaggaatatt ttgctgcatt
    39241 attcaatcgt aacgatattg cggttgtttt aaactgcccg tcgctacatc aggaaaccta
    39301 tgaatctgta gatttgaaaa gtatgctggg tttcgacaat caggtgtttc tattgcttta
    39361 tcagggagtc atgtcatatg gtagaggcct gcccttgatg atagaggctc agaaatatac
    39421 tgaccaaaac atacggtttg ttctcatggg agacgggatg ctcaaaaatg atctgattga
    39481 actgacaaat cggctgaaac ttaacgaaaa agtccggttt atcgatcgcg ttccaacaag
    39541 caaactccta acctatacac gggctgccga tggaggaatc atacttcagg agaccgataa
    39601 gaacctgagt aagaagctgg gaatagccaa caaactattt gaatacatgc atgcaggaat
    39661 tccttttgtg gcgacaaatg ctccggaaaa caatgctgtt gcatcaaaat atgatatctg
    39721 tattattgtt gaaaacaatg tagaaagcat tgcagacgga ataaacaggt tacagcattt
    39781 aaacagggga aatgtaaagg aagaggctct gaaagcatcg caatattaca attgggaaaa
    39841 acaggaggaa actattaaaa agctgattgt ttgatggcct gctttcacaa ctgggtttta
    39901 atattcatta tttctttcta atattgtcaa gataaaccac tacatatgga aatttttgag
    39961 atcaaggaaa aaaacatccg gatttgtgcc aaacacctta gcattggaac cggtgtggaa
    40021 ttcggaaaaa atataaacat ctctgtaaaa gagacgtttt caataggcga tatgagttat
    40081 atcgggccga ataccgaaat gcggggcaga aatattgtaa tcggaaaata tttttacaca
    40141 tccggcggac tacgggtggg tggcggtggt cgtcagcacc cgggggcaaa tctcaccgtc
    40201 ggcgacaggt gtactttttg caataacctg ctaaatgtat gtgaacccat tgttatcggc
    40261 aacgatgtcg gtttgtcgga agatgtttcg cttattacac acggcttctg gctgagtgct
    40321 ctcgacggtt atccggcaga attcagggga ataaccctgc atgacggagt aatcgttggg
    40381 taccggacca tcataatgtt tggtgttgag attggcgagc gtattgtggt tggggcaaac
    40441 tctacagtaa cacgaagtct tacaaagcat ggcgtgtatg ttgggtctcc tgctcggttt
    40501 attaaggaaa tcgttccttt aacacacgaa gaaaaagtaa agaaaactga gcatattatt
    40561 tcggaatacc taaaagttgc cgaatatcat gaacttgcgc caaagataga aatacagtat
    40621 ccagtggtat cggtgaacaa caagtttttt gttaatttcg aaactctcga attcaatggg
    40681 gaagaagata tggtaaccga cgacttccgc gattatgtcc gcaaatgggg gattcgaatt
    40741 ttcaccggca ggaatatggt aagtaatttt aaattcgatt gaaatgatta attttcagaa
    40801 taaaaaaatt ctggtgctgg cgccgcacac cgacgacggc gaattgggtt gcgggggcac
    40861 tattgcgaaa atgcttgacg aaggggccga agtattttat attgcatttt ctactgcgga
    40921 gcaatcgctt cctctgggtt ttgctccggg gactctccga aaagaagttg aaaaagccac
    40981 agggaagctg gggattaaac cagaaaatct gattgtattt tcgtatgaag ttcgtaaatt
    41041 gaattatgta aggcaggaaa tactggaaga acttgtaaaa gttaaaaaat ctcatcgctt
    41101 cgatgttgtt ttcatgccca gccttcacga tatacaccag gatcacacca ctgttgccaa
    41161 cgaagggctt cgggcattca aaacaacaag tatacttgga tatgagttga tatggaacaa
    41221 tctttcgttc gacaccactt gtttcattcg tctcgacgaa aaacatattc aggcaaaaat
    41281 atcatccctg caggaatatc attcgcaagc aggtcgcgat tatacaagcg aggcttttat
    41341 aagatcgctg gcctgtacca gaggcattca gattggcgct acatatgcag aagctttcga
    41401 agtagttcgt tggataattt gaaaataaaa tgagagtact tattcttgca gatacggaat
    41461 acactaacga tccaaggatt atcaggcagg ctgcaagcct tcagaataat tctttcagcg
    41521 ttgtggtgct gtgtctgaaa atgaagggag agaacaatac cggagaaatt cagggcgtta
    41581 cataccaacg attctacgac tcacagatac acgatataaa agcggttacc taccatcagc
    41641 aaatttgcga ctccattctt gagcagtttg aatttgattg tgtgcactgc cacaacgagt
    41701 cgatgctgaa tattgccgta atgctgaaaa aacgaaaacc tgttccaatc atttacgact
    41761 ctcatgaatt atttcatgca tggcctttga atgtttcatc caaaagtttt tcaatcagaa
    41821 ttaaatctat tgtaattagg cgctgggcaa tccgacgcga aaaacacaac agccgttata
    41881 tcgattattg tataaccgta tgtgattcac ttgccgataa tctgtggaaa tattttaaac
    41941 ttcgtgaacg ccctctggta gttcgcaaca taccccagtt taccgaaata aagtcgaaac
    42001 ccaacattct cagagaatac tttaatatta ccgaacaaac aaaaatcttg gtattcattg
    42061 gcatccacat ctaccctaaa accctgaatc tggaacaggt aatcgatgag gttaaaaata
    42121 gggaaaatct ggctctggtg ttcatcacca cctttaacga agggaaaagt gcaattgaaa
    42181 actacgctgt ttcggttggt gctgacaatg tatattttca cgaagccatc ccacctggcc
    42241 aaattccaaa ctatttatct tcggccgatg taggtttggt acctacatgg aataaaaaag
    42301 atttatcgta ctggtacgct ctcgataaca aactgtttga atatatgatt gctgaaattc
    42361 cggtgcttgc cacacaacag ccagaatata caaacatcgt tgatcaatat tgtattggcg
    42421 tgtgtgtaaa ccccgatata aagggttctt atgcggaagg attcaaccaa ataacaagaa
    42481 attattcgct ttatcatgaa aacatcgtta aatcaaagca aatattaaat tgggagaatg
    42541 aagaaaagaa atttttatac ttttaccaaa atttaaattt taatgcctaa ggttattagc
    42601 atacatcaac ctaattactt accttggtta ggtttttttt ataagatgta taagtcggat
    42661 tacttcgtat tctacgataa tatccagata gaacatcgga gccaacaggc atatgtaaac
    42721 cggaccaaaa ttaaaaccaa tcatggggaa ttgtggttaa catgcccgat tcatattaaa
    42781 acagggaagc taattcaaga tgtagaactt gttaaaacca taaactggcg caaaaagcat
    42841 ttaaagactt tatattacaa ttacaatcgc tgcaaatatt ttacacaagt ttatcccgga
    42901 attgagcatt tgattaccca tggcagcaat aatctttgtg aatttaatat tgcaattatt
    42961 aactggatta ttgaatatct ggatttaccc gttaaagtgc tgcgggcttc cgacataccg
    43021 gttaacgaaa ccaaccccac cctacgactt ataaaaatga ttgagttttt gggtgccgat
    43081 gtatatttca gcggcaatgg tgccaggctg tatcagcagg aagaactgtt tgaagagcat
    43141 ggaatagagt tacggtacac caatttcagg cagggtgatt accttcagtg ctacagcggg
    43201 tttatcccca gacttagtgt aattgatgca ttgtttaacc ttggtaaaga aacctcatca
    43261 ttgcttaagc aataaactgc taccaatgat aacgggttat tcaataaaag tttcatcggg
    43321 caaaccttcc gacagaaaac gatgggatca gattgccacc gaaaatgccc agttttgggc
    43381 aagcacctgt ttcgatcaaa ttcaggatat ctatcataaa actccctact attttgagat
    43441 gtttattgat aatactacca tggggggtat taaagtatac gtgtcaaact acaaaaggct
    43501 gaagtacatc tcaaatcgca tcttcagaga agcattcatt cagggcgaaa tcattctcga
    43561 caaaaaattt tcgattgatc atcttcaggt taaaaaaatt tttgaatcat ttctgatata
    43621 ttggttcaga aaacaaaata tcgtttcggt aaaggtttct cccttcaacg gcaacaatga
    43681 attaatgcct gtttttggaa aaattcagct tgaaaaacgg tttaatctgc ttcatatcaa
    43741 tttaaatcaa ccgatggatg aaatatttga taaatttcat ccaaaacatc gaaatctaat
    43801 tagaaaggcc gaaaaaaccg gtattcgaat tactgaagat tataaaccag aaatgtttta
    43861 tgagcagttg ctaaaacaga cgtattcggg tcaagaacat aagccgacgg ataaacgttt
    43921 tatactggcc ttttgcgaat cggcaaaaaa ttacggatat cttaaaatgt actttgcctg
    43981 gcacaacgac accttacttt caggagcatt ggttttggtt tttggagact atggcgatta
    44041 tagcttcggt ggtaatgtga agaacaatct cggcgcgggg atcctattgc aatggcacat
    44101 tatgaaagat ctcaagatac ggggagtggc tacatacagt ttggggcaaa ctgccgatgg
    44161 ttacgacaca gaaaatgaaa aattttcggt aggaatatca aagtttaaac gtcggtttgg
    44221 cggaaaagag cttccggtat ggtcgggttg ctttgttttt aaaccaatat taaaagcaat
    44281 gaaagaaggt ctattaaaag tatttttgaa cccaaaataa tgctgcaatg aaaaaaatac
    44341 ttattctggc ccacgatttt ccgccctata attcaatagg gggacaacgt ccatatagct
    44401 ggtataagta ttttttcaaa catggattat acccggtggt catcacaaga ttctggagct
    44461 ccgacgttat taatacggaa gatttttata aggcttcctc ctttcattcc ataagcactg
    44521 aggaaaccga aacaggaaca gtaatacgca ttccggtaac tccgaatctg cgagaccgct
    44581 tcatattaaa attcgggtac cgccactcga ttattagaaa gttcctttcg tttttttata
    44641 tgtttgctga attgattatt aaatactttg atccaaaatc tgccctgttt cgtcaggcag
    44701 ataccttttt aaaaaccaat catgccgata taattattgc cacaggcgag cctttcatat
    44761 tatttaaata cgcatccatt ctttcgaaaa aatacaatat cccatggatt gctgactacc
    44821 gcgattgctg gagcaacaac cagaatctcg gaaaaattga aaaatttgtt tacagtttat
    44881 tcgaaaaaag atttgtcaaa tcgagtaaat gcattgtcac agtaagcaat ggctatgtta
    44941 ataaaataaa agacgcatcc agacatcctg atattagggt tgtttacaac ggttactgga
    45001 cagagttaat tgatgagccc tgtaacaaac tcaaaaaaga ctattttgaa ctgctataca
    45061 ctggtacaat ttatccgtat caaaatcttg aaatgtttat ttcgggattt cgcaatttta
    45121 ttaccaacca acctgatgca aaaatcagca ttagttttat tggtttagag ttttttccgg
    45181 agcaggtaaa aagacttcaa aatctgattg ttggcatcga aaattacttt gcgacaacac
    45241 ccagaatgca actcagcgat agctttgctt caatgaaaaa tgccgatgca tttctcatgc
    45301 ttgcatcaaa aaaacgattc cagttgtacg ccaaagtttt tgaatacatc gccataaaaa
    45361 agccggtgtt aatggttgtg gatgatggaa atgaggtttc tcgcttttta cgtgatgtaa
    45421 atatcgggta ctttacaaat tcggaagaag acgtttgttt gcatttaact aatctttata
    45481 atatgtggct taacaacgaa ctgcctatgt atgattattc cgccgagaaa ttttcacgtc
    45541 aggttcaagc cgagaaatat gccacgatta taaaacagat acttctgcaa taaaattttt
    45601 gttaatttta taaattaaat attaaactat gcccaaagta gctctgataa cgggaataac
    45661 aggacaagac ggtgcctatc ttgcagaatt attgctaaat aaaggatatg ttgttcatgg
    45721 cataaagcgt cgtagttctc tattcaatac ccaacggata gatcatttat atcaggaccc
    45781 ccatatcgaa aataaaaatt tcattcttca ttatggcgac ctcaccgatt caacaaacct
    45841 gattcgtatt gttcaggaat cgcaacccga tgaaatatat aatctggcag cacaaagtca
    45901 tgttaaggtc agcttcgaaa cacctgaata cactgcaaat tccgacgcat tgggagcatt
    45961 gagactgctt gaagccatca gaatattgaa acttgagaaa aaaacacgtt tttatcaggc
    46021 ttcaacatca gaattatatg ggctagtaca ggaaattccg caaaccgaaa aaacaccgtt
    46081 ttatccccgt agtccatatg cagctgcaaa attgtatgcc tactggataa cggtaaatta
    46141 tcgcgaagcg tacggaattt tcgcttccaa tggaatttta tttaatcacg aatcgccaat
    46201 gaggggcgaa acttttgtta cccgaaaaat tacccgggct gcaacaagaa tagccatggg
    46261 cttacaggac agattatttc tgggaaacct tgattcacaa cgcgactggg ggcatgccct
    46321 cgattatgta aaggcaatgt atctgattat gcagcatggt aaaccggatg attttgtagt
    46381 ggctaccgga gtaactacta aaatacgcga ttttgttatt atggcttttg ccgaattggg
    46441 aatttcgctt gaattccgtg gaatcggagt ccacgaaaaa gcttatgttg cagaatgtta
    46501 caatcccgca tatgctgtta aaaaagggca gtgtgttctc gaaattgacc cgggttattt
    46561 cagacccact gaagttgaac tattgatagg agatgcaacc aaagcacgta cagaacttgg
    46621 ctggataccg gaatattcac tcgatctgct ggtaaaggaa atgattgctc acgatctgaa
    46681 attactgaaa aacaaccaga actattttta aaatggatga aacttcaaaa atatatgtag
    46741 ccggccacac gggtatggta ggatctgcta tcatgcgtca gcttagaagt aagggttaca
    46801 gtaatctgtg ttacaaagac atcactgagc tagaccttac aaatcagcaa caagtacttg
    46861 agtttttctc cgcagaaaaa ccggaatatg tgatactggc tgctgccaag gtaggtggaa
    46921 ttgtggccaa caatacttac cgagcccagt ttatctacga aaatttgatg atccagaaca
    46981 atattattca tcagagttat ttacatggta ttaaaaaact cttgttcctg ggcagtagtt
    47041 gtatatatcc caaattagca ccacaaccac ttaccgaaga ttatctgctt accggtgaac
    47101 tggagcccac aaacgaaccg tatgcaatag ccaaaattgc cggtatcaag atgtgcgaag
    47161 catatcgcga ccaatatggc tgtaatttca tttcggttat gccaaccaat ttatatggcc
    47221 caaacgataa tttcgacctc aataattcgc atgttttacc ggctatgcta cgcaagtttc
    47281 atgaagccaa attaaacaat tcggaacagg ttgttttgtg gggaacgggg tctccgctgc
    47341 gtgaatttat gcacgtcgac gatatggcag cggcatgcat ttttctgttg gaaaaataca
    47401 acggccggca atttgttaat attggtgtgg gtgaagatat cagcattaaa gagcttgcaa
    47461 atgtgatttg taagattacc ggttttaatg gcactattgt ctgggataca acaaaacctg
    47521 atggcactcc gcgaaaacta ttaaatgtta acaagttaca ttctatggga tttcgtcatt
    47581 ccatcgcatt agaacaaggc attgaaagtg tttacaaaaa cgttttccta agcaattcat
    47641 gaaaaaagta ctgcttttgg catacgattt tccaccattc aactccgttg gggcttttcg
    47701 cccttatgca tggatgaagt attttcatga gtttggggtt tatccatttg tggtttgccg
    47761 aaactgggat gaaattggcc accagccatc gctctataca aaaccgtccc ttcagaaaga
    47821 agccattgtt gaaaccacac ctgtatatac gcttattaaa gctccataca gaggtaactt
    47881 cagggacagg ttaatttaca gtcatggaat taaaagcggt ttaatacgga aattattaag
    47941 ccttatttat tccatttggg agtttcactc gttgcgttgc gatgcaagaa gtgaaatatt
    48001 ttacgaagct gaaaaggtaa tttcaaataa taaaattgat tttatcatag cttccggaga
    48061 gccatttgtt cttttcagat atgctcattt actttcgaag gcgcacaata ttccttggat
    48121 tgccgattat cgcgatgcct ggacaacaaa tttcaacatg tacgctttta atccggtaga
    48181 gcggtttatc aaggccaaaa tcatacggcg gcatgaaaaa atgattgtga gttcagcaca
    48241 tttgatcact acagtatcgg aggggttggt tacaaatttg cgcagcattc attttaataa
    48301 agaaatcaaa attattatga atggatattt tcatgaagat ttcgaagagc taaaaaaaca
    48361 aacatcccaa aataaaaaaa tggtaattgc atactcggga agtctgtatc cctatcaacg
    48421 gcttgaagtt tttctggatg ctttgtttac tcttatcgaa atatcgggag agctacctgt
    48481 agaagttaag ttttatggta tgaaattaca tcctgaacgt aaaaaatcga taatagagaa
    48541 acaccctcaa tttgaaaaat attgttcttt tattccggtg ctaccgcgcc gggaacttct
    48601 cgaaaagttg gtcgaagcag atgttttgct tttgctggca aatgaaaaaa tcgatggttc
    48661 ctgcgctaag gtctttgagt atatagtttt acaaaaacct attcttcttt cggtcaacga
    48721 ccatgcaact ctcgaaacgc tattgaaaag caacaagaat gcatatctgt gcgaaaatac
    48781 tgcggaagtt gccaatgcat tacgttcact actgcgtgaa aaaaccgaaa ccggacaaat
    48841 acagtcattt gaattcgatt attttgcata tagccgcaga aaccaaaccg aaaaattagc
    48901 ggctctgctt caataaaaca ggtataaaaa tgactaaact gcaaactgtt ctggtttgcg
    48961 ggaatgtcgc ttgatagcaa accatttata gatatttaca atcacccatg cggcaggaac
    49021 aagcataagt atatcgttat tcaggaaata gcgcatttcg atatttcgaa ctgtaaagca
    49081 gatgtaatag tacactacag caaaaaacag aaaaatgaac aatccgtaaa ggtttttctt
    49141 tcgtaacatt aaatacactc cgataaatcc aataagaatc agcaacgacc ggtacaaaaa
    49201 gagcattgtt atagccaacc cgaaaatccc ctttttttcg ttgaccatgc tggtcttaaa
    49261 aagtgctttt ctcaaatttt gcaatgggat aattatctta tagtgaaaag gatgggcctt
    49321 aatctgattt gtcctaagtt tactaaaata atatacaata gaatcgcaac atgattcttc
    49381 tattggtatt tgggcattgt ctttgttcca gtattcacgc cagtagctga agccataacc
    49441 gcaatttttt gcgagatatt cagctcttag caacattaat gaatcttctt tcgacacaaa
    49501 agctatttcg ggccactcaa ctttttcgtt gcggacaatc tgaaaaaact gaggattcca
    49561 gtcgctttgc aaagaataga tatatttacg acaagcaacc acatcttttg aaaaggtttc
    49621 catgccaccc cgggaatcgg aaaatggaat aaatttatcg agcaggatat agtttctggt
    49681 tatccatgat ccgtatacac cggaaaaacc aattaaagca agggttattg gcaacagtgc
    49741 aaatttaaga ttacgaatat tgtagagcag atatacaaaa acaaatacag gaataaatat
    49801 caggttttga ggccggcaaa gtgcacttaa cgcaataaaa acacctccga ataaaaaatc
    49861 agttttattt cttttgcgcg ctagaaacat taacccaagc agcatggtaa aaattcccat
    49921 tgtttcggat gaaataagag gcacccatat tattgcaaaa gggtacagtg catatattaa
    49981 ggcggtaaca ataccaagcc tgtagctttt cgaaatgaag taacaaagtt gatacaaaat
    50041 aaatacgctg gcaacatcga gtactatctg tatccaaatc attatctgat acattaagtc
    50101 aacatcgttc ccggatatca gataaacggc cgtcatcaac aatgggtatg cgggtaatct
    50161 tgctgcaacc gatttaggat catccaaaat accacggaat gttccatgtt cagccaggtt
    50221 ttttgcacac tgcgtatatg aatacgtgtc tcctgtttcg aagaaattct gttttccgta
    50281 atatagttct gcaccgaaca atacaaaaac caccctgaca aggagtgctg tcagcacaat
    50341 cacaacaatg gctttagagt atttcttaag catgaatgtg taaggcattt aacttagtaa
    50401 cacaaatata taataaaaaa acaatattac acaaccggtg ccgaaacatc ggattttacc
    50461 gattctacct ctggcttttc acgctcaagc cttcgttctg ctctgaaatt attatacgtt
    50521 attagcaatg ctgtaaaaat gaatggcata tagggaattt tgtatcttac caaagctcca
    50581 aaattggcag tagtaaggcc gacgatatac gataaggtaa tcgaaaaagt gagtgcaaaa
    50641 attacaaagt ggttgccggt aagttgcttt ccaaaatttt tgatacctct gccaaaaatt
    50701 gcaaacatca gcaacgcaaa tacaaaggtg ttttccaaag ccgacaacat gttgaacgga
    50761 ctgcgtgcct cccatgggaa tggcctgaaa aaacctgaca caatagcgat gggggctttg
    50821 cttaaaattc cagcatatga tgcatcaaag ctacctatat tgaaactatt accgccataa
    50881 taatcttggg taaggtcgtt ttgaacaatg gctgcacgtt ccatcatcgc ctccggtgag
    50941 ctataaaact ctcctgctgc agatgaaaat cgtgtcattg caacagtgcc ccccaaagcg
    51001 gcaacaatta aaataacagg aaatacaaat agtcgtataa acttgctttt aattttttgt
    51061 atgtatccga acgagaacca aatcgtcatg cctaaaataa gtgtcatcaa tatatatggc
    51121 tttaacgata ttagaataaa aacagaaaaa acaatgtaaa aaagattccc caccaatcgc
    51181 ttacgatcga attttattat tgcattgtag aagcctgccg caaaccacaa ggtagctgcc
    51241 atggtgtatg agtcttttaa aataccgcta ccccaaaata ctaccgaagg tatcagaaaa
    51301 agtccataag ccaacgtttt cgataaatgc ggatagagag agcaaagaac tttataaaac
    51361 ttccaaaaac cgccgaacaa aaatatattt aagaagattg ttgtccataa ataagatttt
    51421 ccccctaaaa aaacaaatat tgatgtgaaa cggacaaccg caaatgcttt gggatcgcgg
    51481 gtgtaaaacg gaaactcagt tatagaatta aaggccgaaa gagcttccgg gcttcggtct
    51541 cccaataaaa tttttaaata tgaatcaaaa tcgtgttgtg ctaaatttac caaagcctta
    51601 gcactccaat aatacaatgt tgtatcgcct ccattataaa aaccggtata aacaaggcag
    51661 aaaagcactc ccccaagcac ttttaccatc agtcccagag gatagtattt gtaatatggg
    51721 tgttctttta tattcctttg ccttatgaac ataccaaagg cccatatcgc tatcagcaag
    51781 agtaaaagaa aagtaaaatc ccaaccagta tagtagaaat caaacgttct tctgaagcct
    51841 tctatattaa acatttacaa caagcttacg attggttaat aactttacaa attctttgaa
    51901 ggttgctatt atcaagatca aaatacaaag ggagtcgaag caggcaagac tcatatctgt
    51961 cgcagttcac taagtctttt ggggattcaa tcttgctgta gaatttactt ttattcagtg
    52021 caagataatg ggtaacggca agtacatcgt ttgttttcaa attccctgta atacgtattc
    52081 gttcagcttc ggaattacaa ataatgtaaa acatatgggc attgttggtt gcataatcag
    52141 gaattaccgg taacgaaaac ttgttggtct gataattaaa aagagtatcg taataatacg
    52201 cccaaattgc tttccttttt tcctgaatgt ttttataatc ttcaatctgt gcccaaagaa
    52261 atgcagaaat aacttcggaa ggcaaaaacg atgaccctat gtcgacccag ccatatttgt
    52321 tgacttctcc tctgaaaaat tctgcccggt tggttccttt ttcccaaata atttctgccc
    52381 gcttcataaa cctttcgtcg ttgatggcaa gcatcccacc ttcgccacac tgtatattct
    52441 ttgtttcgtg aaaggagaag catcccaaat gtccaatacg gccaaggggt tgccctttgt
    52501 agtaagaatc gattgcctgt gcggcatctt caacaaccca taatttatat ttatctgcca
    52561 ggttcatgat tttatccata tcacaggcaa caccggcata atgcacagga actattactt
    52621 ttgttcttgg agtaatgagt ttttcaattc cggtttcgtc gataccagga tgatcgggtc
    52681 tgctatcagc aaaaacaatt tttgcaccct gcctaacaaa agcaagtgct gtgcttacaa
    52741 atgtataggc gggtacaata acttcgtccc ctggtttaat atcaagcaaa agcgcagcca
    52801 tttcgagagc atcggtacac gaagtggtca tcagagtttt cttgaaaccc cagatttctt
    52861 caaaaaatga ttggcatttt tttgtgtatt ttccattccc tgatatttta cccgatgcca
    52921 ctgcatcgta aatgtagtgc acttcttttc cggtaatgtg gggtttatta aatggtatca
    52981 tctgaatttg ctgtaagtta taagttattc ctgtattttt tttatcagtt tggccgggtt
    53041 ccctgcaacg atgctgaaag gttctacatc agttacaact acagagctca ttccaatgat
    53101 actataatct ccaaccttga tatcttcacg aacggtactg ttcagtccga catgggctcc
    53161 tgttccaatc cgtatcctcg cacctaaaac tccatgggcg gcaacagtag cataatcttc
    53221 tacaatagta ttgtgaccaa taaagccatt ggtgtacaca tgaataaaat ttttaacttc
    53281 agcataggcc gatgttgccg catagggaag tattaaagaa ccgcagccaa ttttcgatgt
    53341 cgggttaata tatgcctggg ggtgaatgcc tgttgcattg gcttcaagcg gaatgttaag
    53401 cttttcgaaa tcaattaccc gccggttttt catttttgca ttgaaatgat aggtataatg
    53461 gatgtaatag ccattattta caaaatcaag aatcgactca gtgttcccaa ttactttatg
    53521 ctttcccaaa taattgccaa ccggaacaac atcgttaata aaaccggcaa tttcccattc
    53581 accggtttgt tgattaatgt cttcaaaaac cgtcatggca atttcgccgc taccatgagc
    53641 tccaacaatt attaatcgct ttttcattta attacatctt taataatata cggtctgtaa
    53701 tttgaaattt cccgtaaaat acgcttaata tactccccaa aaacaaaaaa caaaatcaga
    53761 ataagtgtat tataaaacga tattaaaaca attaaactgg tccatcctgc aggtgcattg
    53821 cctaaaaata actgcttaat aacaaagaca ccactgatta tcaatgaaat aaccgaaaca
    53881 aataacccaa gatatccgaa aattttcagt ggtatcgagg aatagtttat cagaagatta
    53941 aacgacaggt taacaagttt ttttaggcta taccctgatt tcccgtgttg ccgtgcttcg
    54001 tgtttgatta tctgatttgt gaccttcgaa gtggtttcta atatcatacc cgatatgtat
    54061 gggtatgggg ttcgtaatga ttttatctga tcaataatgt cttttttaat aatccgataa
    54121 ctcgaaaatc ttaaatcgcc tttaacattg aaaatggcat ggtttagttt tcgtaagatt
    54181 cgacttccaa gatttttgtg aaatttgtcc tttttctttt cgtagcatcc aaataccgca
    54241 tcatagccct gatttatttt ttcaaccaaa aggggaattt cctccggagg attttgtaaa
    54301 tcgtcgtcaa tagtaaccac aaagtttccg gaacaaaatt gaaacccgca catcaaggca
    54361 ttttgctggc caaagttgcg gtacaaatcg acaataatga tgttcttccc aaatattgtt
    54421 ctgatttctt tcaatacttg taagctgttg tcgttgctac aatcattcac aaaaataatt
    54481 tcgaattcac tccgcagatt attaaaaact gccaccaatc gctctgtgag tggtttaaga
    54541 gtatgttcac tattataaac gggcactatt actgagaaag tattcatatt acaggccaag
    54601 atcttggtta attaatataa ggtgggtccg gtctttatat ccctccattt cgccatcaat
    54661 tattactgtt ttcaaacaac cccttcttgg ggagttgcag tcggagcaat agcctgtttt
    54721 catacaggga ttgtcgaaac gattgtgatg tttaatatta tgacggtggt aatttagcgg
    54781 tgctgcaatg tccctgttac ggcgaattgc gtcttcaaga ttgcgggtaa ttttatttcg
    54841 ccctacaaca ataatcactt ttctaggtcc aaaaatcata cccccgaccc tgtttccggt
    54901 tccatctgtc gaaacaatct gcccgttcat ggtgatggca tttgctccaa taataaaaat
    54961 atcggatagc aaggtttccc gcattttctg ccatagtttc tccattaatg cgtaatattc
    55021 ttctttagga agattcagct ttccatcggg ttggtcgcca aaaatggtat atataccctg
    55081 attatttcgt tccagtggat tgttaatgct taaaccgggt attttgccga cagtatcgta
    55141 agcattcatt tgatgtagtg taacactatc ggaaaaccca acctgagtag gaggtgtatt
    55201 gcttttgaat gcttctacaa gcgatgtaag tgttttctgc gcttctataa cactactaca
    55261 tgcataaaaa ttgatacgac gtgacgtaaa tttcttttta agtgtttccg ccatcgcgat
    55321 aaaatgcatt tcctgttcct gaataatatt tttttctgcg tccataatct gctaaagtta
    55381 atttttaatt ctttccatat tccttgtctt gccgctttgt gtatcaatcg ggaattcatc
    55441 gacaaactca atgctgataa ttttatcggg atagcgtttt tgccaccgct gcaatgcttt
    55501 tgccttaatt tcatttttgt cgaccgagtt tttaaccttt aattgcaacg taatactctt
    55561 atcggggtac tgcacaaatt taaagtgttc acattcatgg aaatccataa acatttcgtg
    55621 ggcatgatga tgggcaaaca tcgttccatc aggaaattca aaaatatcat ccaaacggcc
    55681 aataaactta ccaaggtatt taactccgaa atcaggatga tcttcaacat acgcaatatc
    55741 cttagtattg tagcgtataa ttggcattgt gaagttaatc agattggtaa gtactggagt
    55801 ggcaaggtgc tgattatttt cagtacgatg atcgataaat tcaaacatgg ctgagtttgg
    55861 aaagagacga aatttatttt ccatatttat gtcaaagcca atagtgggtg attccataca
    55921 gccgtaatag ttcaccaaat tcttacccaa agtatcgtaa aaaagctgag cctgcttgtc
    55981 gaataccgtt tcagatgtaa aacaaacata aggcgtattg agttttattc ctttttcctg
    56041 catcttataa gaaattatct gaagcatcga aggtgtcgac catagaatat atggtttttc
    56101 tttttgtatt ttttcgacta ttttttcaat tggctcatac accgatacaa tttgccttct
    56161 aaaaaacggt aatagttttt gaatagtcct gaaccatttc agatcttttt cgtactgata
    56221 tacagcatct ggttcataac gggttacaat aaggactttt ttcaatggtt gataaccata
    56281 ttgcctcaac gaccaaaaat gccgcacctg attggtgaaa tcttcccttt tattgtaggc
    56341 aatcctgaaa ggttcccctg ttgaaccact tgtacttcga atgttgaatt tccttgggtt
    56401 tgaaacatcg acggtggcaa tatcctgcaa agaataatta cgtaaaaccg atttatcaat
    56461 caccggaagt ctttcaatat ccttcatcga tttaatttcg agattaaaaa cctgatgctt
    56521 ttgatataag tctttataaa atgcagaatg cgtttttgcg tgctggaaaa ttgcattaaa
    56581 gcgaattatc tgacgtcgcc tgatttcggg tgaatcaagt cttttccaga ttgatatctg
    56641 gttgtaatat ttaagcagat acagcaacat cagacatggt gtttattcat gatttttgtg
    56701 tgcttcacca gtactttttt agctgcaatt accataggag gtccaataaa tttaccattc
    56761 attaatgcca ctcctttgcc ctctttttcg gcttcttccg acaaccggag catttctttg
    56821 gcatcaaaaa cttcctgctc tgatggcgag aaatatttat gtgccagtgc tatttccttc
    56881 ggattaagta taagcattcc ttcgaagcca agtttttttg caagtctgag attaatttcg
    56941 aggtcatcga gatcatgaac attaatatgc acggtgtcaa ccggaattac tccaacagcc
    57001 cgtgcggcca tggcaatcat ggcgcgagct gtaaacaggc tttcgtgttc cgggtcgtgt
    57061 atgccttcca aatccgaaat aaaatcctcg cagccaaaag caatggcaat gaccctgtct
    57121 gatgctttgc atatatcttg tgcattaagt actgctgctg cggtttcgat tagtggtata
    57181 attttaaatt tacctaccgg aagtcccttt tcatattcaa tcgtttccag aagcttatcg
    57241 aaaaaatata tatcctgtcc tgttttcgct tttggataca taaaaccatc aactccctca
    57301 atgctcaaag cttctacatc tttaagcagg tgtccacttt cccgatcgtt gatccgggga
    57361 aaaacaaggt gattcttgaa tttaccttca ctaacccatt gctttaccat tgtgcgagcc
    57421 agagctttat tagcgactgg ctgcacagaa tcttcaatgt cgagaagcaa tacgtcggct
    57481 tcactacgcg aagcactgct cagcaatcgt tcgttgtggc cgggaacaaa catcagactt
    57541 cttaaaagat aatgattcat attgtttttc gtttttaggg gaacatcggg ttttataaaa
    57601 actcaattga taagggaaat atattaacaa ccgtgatatt ctgattaaat tattttcaat
    57661 gcattgcata ccgacaatag tttgcttttt tatgaactgt ttcagacgca aaacacaaag
    57721 ataatctatc ggagcaatca aaaaaacatt ttcctgaata ttcatacgat cctgttttac
    57781 atgctttgcc ttaccggttt gggcatacag agtttttagt aacgaaattc ttcagaagct
    57841 atttcaggtt ttgcaatgca agctttttat aggttgaaat gaatagggac aggcaaacaa
    57901 tttccatcag gaaataagta atgcaaatgg aagcagcaca acccacaata ccataagtct
    57961 gagtgagaaa aatactgagt ggaacaaaaa ccactgcacc cagtactgta gaataactca
    58021 tgtatctttg tttaccgtaa tgcacaaaaa aactgctgtt ggcagtaaat aataccctaa
    58081 aactcatggc aaacgaaagc catggaataa caaccgctgc ttttgcaaat gcaggggtgg
    58141 tcatcagcga tattatccat ggggaaacaa aactaagtac aatgctaaga ataaacagtg
    58201 aaatagcaat aaaaatttgt gatttaacaa taaaatccct cgattttcga aagctttgtt
    58261 ttgcaagacg ttcgtaagca attggtgacc aagtattaat gaatgaaagt cctagaaaaa
    58321 aaattaccat agctatagtg gaacccaccg aaaacaaacc gacttcggct ttattcagat
    58381 aagtgttaat aataattttc ccgctttcaa ttataatgac tgttgccaga gcatgaggcc
    58441 acaacgaaag tgagtttttt agaatatctt ccctgtgttg tttcgatggt aaaacccgaa
    58501 tgttattctt tttccacaat aaaagaatgg ctaacaatcc cataattata atactgacaa
    58561 tcgttccggt aacacgtccg gcccagttca tatgaaaagc gattaccaac aatagtgaca
    58621 tactcagatc aataacactc cgcgaaatat tgattccagc aaataatttg gcttgttttg
    58681 tcattcgaag ccgcactgtg taaaattcga atccaataaa aaagaacgaa attgcaggta
    58741 ttattacgaa taaaggctcg ggaatcagtt gtatttcggt aaaaaaacta ataacgaaca
    58801 acaacaaaac cccactgccg gcaataatca gaagtattaa aaacagatta aacatttgaa
    58861 ctcttttgta ttcatcgtct ctgttgaaaa agaactttga tattgtactg cttaaccccc
    58921 ctccgataag tgcatttgaa atcaggaaca acgccctgaa attattgata tatccataat
    58981 cgacaggtga aagataaatg gtaagcagcg gtagtaacaa aaaaggcaat gcttcgccaa
    59041 tcattgtggt tgaagcgtaa tacccaaaat ttatcaggct tttttttctt tcgggtgtaa
    59101 ccgtattttt aaactgaaaa ttcaaaataa ttgccttaat tgaatacctt tgcggtcaaa
    59161 gatagaaact aattgatttt attgtatcaa tatttctgaa gaaacttatc tgcaagccat
    59221 catgaaagca atttacaata cctgcgttag cgaccctttt ctgaaagtag cgatacagct
    59281 ttgcgaaaaa tacaatattg aacccatcta ctggatcgga tatcacaacg atgcttcgga
    59341 acaagatgtg caaatcactt ttccggatat aatatatcat cgctattatg atgcatggac
    59401 cggtattctg cctgaaaaaa cccgacactt tgctgatgaa actccagagg atatcgactt
    59461 tattaattca cattgtttcg aagaaattaa tgcgttgtcg attatggatc ggatggatcc
    59521 cgaccgtttt agttttcctt ttgcagagcg acaaatgctg tatcgtaaat atatcagata
    59581 ctggacagga tttatcaaat caaagaaacc cgatgtactg ataagtgcaa tatatcctca
    59641 tcgaatctat gattatcctt tatatgcatt gtgcaggcat ttcggaatta aaataatctc
    59701 attctatccg gtttccattc cgagtcgtat tattccgata aacagtattg ataattttgg
    59761 tacaaagatt aaagaaaaat atagagaact aatttcctct ccgaacgcag atctatcgct
    59821 gtcgaaagat atagaaggat attttcagaa gttacgtggt gcctacgata cggcatcgcc
    59881 atattacata aaaatcaacg caaaccgcga caagcagaca tcaaatgttt ttaaagctgc
    59941 ctgggttttt ctgaaaaatc caaaattcta caacgaaaaa aacacaaaaa ttctgtttca
    60001 tggtatcgaa ttactcttca agcagaaaaa taaagatgtt gaagacagca aacgatatcc
    60061 gtttattgct tatatgcgta ttctactcaa gaaacgattg atgcagaaac gacttcgcaa
    60121 attctatgat tctattgcgc agaagccgga tctcacccaa aagtacatct attttgcaat
    60181 gcactaccag cccgaagctt caactactcc aatcgggggt gtgtttaccg agcagctaat
    60241 ggctatcagg caattatcag cagccattcc cgataactgg aaaatatatg taaaagaaaa
    60301 tttttatcag tttgtcaatt attcacatgg ccatctggga agaatcaaga aacactatac
    60361 cgatgcattg agcataccaa aagtggtgtt ggtaccaacc agcattccct cgtttgaact
    60421 agccgataat gcaatggcag tttcgaccat tacgggtacg gttggcatag aaagtgttgt
    60481 gcgcggtaaa ccagtaatcg tgttcgggtt aacctgggtc gaagattttg acggagtact
    60541 caaaataacc agtaataaag acgccgaacg catcatgcct tttatacaat cgtttgtaca
    60601 tgatgaacac aaggtaaatt gctacataaa agctttcgat atggcttcgg tttacgcata
    60661 tcattatccc gggaaaaaac tgaggtttaa tatggatgag gagctatgcg taaagaacct
    60721 aattgaatcg attgcaatgc aagtttagtc aacatcatcc attgaaagtg cgctaccttt
    60781 ttgaatattt gttctggctt tttttccgat aatttcttcg ataaacttag gatgtaaacc
    60841 tgctcccggt ctgatggacc ttatattttc acttgtcagc gtttcgcctt tccctatatc
    60901 tttaacaaca aacagcgaac gtcgccggct tttatctttt tcactcacca cataacttat
    60961 ttcgcctttc gctttttcgg caaggcgcac agtgttaacc atttctttaa attcatgggg
    61021 ttccatcgaa aaccctgaat cggggcctcc aagcttccgt tcaagaatga aatgcttttc
    61081 tacaattacc gctcccagcg aaacagcaac cactggaact atgctcccca ttgtgtgatc
    61141 ggatactccg acaggcactt tatatttatt gatcatatcg ggaatggtca gtaaattggc
    61201 tttttcaaca ggggcaggat attccgatgt acatttcagt aacgaaacct gatggtttcc
    61261 cctgagatag catgtttgta atgcaagttc aatatcgtcg ggttctgcta cgcctgttga
    61321 aattattatt ggtttttgac atgatgcaat atatgatatc aaaggaatat cggtaatttc
    61381 catggatgca attttgtaca taccaacccc aatgctctcc agaaaatcaa cagcctctaa
    61441 gtcgaatgga gatgaaaaaa acagcattcc aaaatcttca gcaacttttt taagtttggg
    61501 ttgccattcg tatggcatag ctgcctcctg
//
LOCUS       DAOOQP010000005        61195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_537_length_61195_cov_5.482270, whole genome shotgun sequence.
ACCESSION   DAOOQP010000005 DAOOQP010000000
VERSION     DAOOQP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 61195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61195
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_537_length_61195_cov_5.482270"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLK75_01030"
     CDS             complement(<1..310)
                     /locus_tag="PLK75_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOY37858.1"
                     /translation="MDNSIIAAIDIGTTKIVAITGKRNSENKYEILGFGTVPSKGIKR
                     GSVLNIEEAIETIRLAVEKASEISGIKLSEVYVGIAGQHIRSMPARTTVSFGSPDHEI
                     V"
     gene            complement(321..1115)
                     /locus_tag="PLK75_01035"
     CDS             complement(321..1115)
                     /locus_tag="PLK75_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37859.1"
                     /translation="MKNKIIKILKWSLLIAYLIVVMSFVDKMRTKALCSSLRITVNSE
                     SEFIDSADVNKLLTNNKLFYDSIPLSHINFELIEKTIKNHSAVRDVQVYSSYEGKLIV
                     SVVQRVPIVRVIPKYTSLNNGFYIDDQGKIMPLSEKYTAHVPVITGEITPQFISSIDN
                     DSVKNRNVIETYHFTLYDLYHFVSYIHHNELWSPQFEQININANYEVELVPRVGNHII
                     VLGDLTDYEYKLFKLEAMYYEGFKHTDWNSYKTINLKYANQIVCTK"
     gene            complement(1105..2490)
                     /gene="murC"
                     /locus_tag="PLK75_01040"
     CDS             complement(1105..2490)
                     /gene="murC"
                     /locus_tag="PLK75_01040"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOY37860.1"
                     /translation="MKDQSNIAYYFIGIGGIGMSALAHYFSKQGYFVAGYDRSRSEIT
                     DALADDGISMCFDEDLESIPAQCHDKFCCRVVFTPAVPPSHKQLEYFRSNGFEVLKRS
                     EILAEIAALKTCIAVAGTHGKTSISTLAAWILNQGKLKAGAFLGGISGNFQSNLVLHK
                     NSELVVVEADEYDRSFLKLFPDIALISYIDADHLDIYGNAQNMENAYNQFANQTKHNG
                     TVILKNDIRNKVVLTGGQKLLTYSFSDVNSDFYANNITITHSRCHFDLHTPFGVISEI
                     VFPYAGRHHVENAIASSAIALCAGADAGHVRNALSCFKGVKRRFEYIINTDKLVFIDD
                     YAHHPREIAATIEAARLLFPEWKITGIFQPHLYTRTRDFANDFAESLSKLDRLILLDI
                     YPARELPIEGVSSEIILEKVTIADKITCTKSTLPGLVEKCIKTEVFITMGAGDIDKMV
                     QPIKNILLNEK"
     gene            complement(2474..3586)
                     /gene="murG"
                     /locus_tag="PLK75_01045"
     CDS             complement(2474..3586)
                     /gene="murG"
                     /locus_tag="PLK75_01045"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOY37861.1"
                     /translation="MSPKKVIISGGGTGGHIFPAISIANALKVIESDIEILFVGALGR
                     MEMEKVPKAGYPIVGLPVMGFPRKLSLKMLKFFTGLLKSTQQARKIVDEFKPNAVVGV
                     GGYASGPLLRVASRRGIPCLIQEQNSYPGITNRMLAKKVRCICVAYEGLDKYFPAEKL
                     ILTGNPVRQDLIELDDKKREAVEFFGLDPELPVVLSLGGSLGAYTINEAIGNNISVFE
                     NQKIQVLWQTGKSYIGKAEEVIRTGKSNYIKAFDFIYRMDLAFTVADIVVSRAGASTI
                     SELCVIGKPAIFVPSPNVSEDHQTKNAMALVNKDAALMVKDIEASYELAPAILSLVGN
                     KELQNKLSENIQKMAILNSAETIAKKVIEIADEGSK"
     gene            complement(3606..4871)
                     /locus_tag="PLK75_01050"
     CDS             complement(3606..4871)
                     /locus_tag="PLK75_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOY37862.1"
                     /translation="MLEFIKRNIKGDRVIWTILIILSFYSLLAVYSASGTLAYKYFGG
                     NATHYLLRQGALLFVGFLIIFFVHRIPYRYFSKLSQFAYYLAVFLLLVTLLWGISLNQ
                     ASRWIVLPGGITFQTSDFAKVALVMYLARMLAVHEDDIGTFKGMLRYLMLPILIICGL
                     IFPAGISTTLLLFGISMLLLFIGQVKFKYILSVLGIALAGLGLFVLIIQVSPIESRLG
                     TAQKRIEDFMNPESEGNFQANQSKIAVASGGFLGKGPGNSVQRNVLPHPYSDFIFAII
                     IEEYGFFLGGLVLIALYIFLFYRVVIIIRKVDRKFGAFLVAGLGLLLVFQALSHMAVA
                     VNIFPVTGQTLPFVSMGGTSILFLSLAMGVILSVSREVNELEEAKKKEINESPDPPAV
                     TEDSSVPEEDTETREETVAAGVIKELNIK"
     gene            complement(4871..6214)
                     /gene="murD"
                     /locus_tag="PLK75_01055"
     CDS             complement(4871..6214)
                     /gene="murD"
                     /locus_tag="PLK75_01055"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOY37863.1"
                     /translation="MKKKIVILGAGESGVGSAVLALAKGYDVFVSDMSMIAPKYKIML
                     QDYNIGFEEGKHSETLIYSASEVIKSPGIPDKAPIIKELHARNIPVISEIEFASRYTS
                     AIKICITGSNGKTTTTMLTYHILKKAGFNVGLAGNVGQSFAFQVATCNYDYYVLELSS
                     FQLDGMFDFKAELAILLNITPDHLDRYEYKFQNYVDSKFRIMQNQTENDVFIYCSDDQ
                     VIINELEKYNTPARLYPFSLNDTSITNGAYTNNEQINLMDNSKTAMTMTIHDLAIQGK
                     HNVYNSMAASIAAHVLDIRKDIIRESLMDFKNVEHRLEPVIKVHGVEFINDSKATNVN
                     SAWYALECMKSPVVWIVGGVDKGNDYSGLVDLVKQKVKAIVCLGVDNSKIHAAFKEYV
                     PQMVDTKSMEEAVNAAYHLASNGDSVLLSPACASFDLFSNYEDRGYQFKKIVRSL"
     gene            complement(6217..7473)
                     /gene="mraY"
                     /locus_tag="PLK75_01060"
     CDS             complement(6217..7473)
                     /gene="mraY"
                     /locus_tag="PLK75_01060"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOY37864.1"
                     /translation="MLYYLFHYLDQLNVPGAGVIEYISFRAAMTVITSLVISMLFGKK
                     IIRKLQRLQIGEEIRDLGLEGQMQKKGTPTMGGVIILLAIILPVLLWAKLDNTYVILM
                     LATTIWLGGIGFIDDYIKVFKKNKKGLPGKFKIMGQVIIGLVIGLTLYFSDDVVIRVK
                     SQTPATADQQIIGDDITISNCEEGDVLYHDIKSTRTSIPFVKDNEFNYVWFVNFLNEN
                     VKYKFAWGIFVVITVLIITLLSNGANLTDGLDGLAAGSAAVIGATLGVLAYVSGNHIA
                     ADYLNIMYIPNTGELVVYMAAFIGATIGFLWYNSFPAQVFMGDTGSLAIGGIIAVFAI
                     IIKKELLLPILCGVFIMESLSVIMQVAYFKYTKRKYGEGRRIFLMSPLHHHYQKKGYP
                     EAKIVARFWIVGLLLAVITIITLKLR"
     gene            complement(7473..8933)
                     /locus_tag="PLK75_01065"
     CDS             complement(7473..8933)
                     /locus_tag="PLK75_01065"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOY37865.1"
                     /translation="MKRIEDLCKYVAVKQIVANGVESFANVVFDSRIVMQDDLFVAVK
                     GTVSDGHSFIQDVCAKGAAAIVCEKLPEDLNNNVCWILVNDASLALAEIAAFYYDFPS
                     EKLKLVGVTGTNGKTTIATTLYKLTLKLGYKAGLLSTVRNMICADEVLATHTTPDALQ
                     LNKLLREMVRQGCEYCFMEVSSHSVVQNRIAALKFTGGIFTNITHDHLDYHKTFDQYI
                     KAKKAFFDMLPSSSFAITNNDDKNGEVMLQNTSAARSSYSLDRVADFKAKIIESHFDG
                     MLLSIDGHEIWTHFVGRFNASNLLAVYGAAIKLGFQREEILIVLSTLHAVDGRFQTIK
                     SSSGIIAIVDYAHTPDALKNVLTTIQQIRSKDESIITVVGAGGNRDKTKRPEMAKVAS
                     LLSDRLILTSDNPRNEVPEQIISDMEQGLSESAKANTLKIVDRKEAIRTACILAKKGD
                     VILVAGKGHENYQEIMGVKHPFDDREIVTEIFKTLK"
     gene            complement(8930..11029)
                     /locus_tag="PLK75_01070"
     CDS             complement(8930..11029)
                     /locus_tag="PLK75_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOY37866.1"
                     /translation="MTPRTEILWRIKVIYFIILFFGLFIFVKIFRLYFTIDETVITNA
                     SKSVTRVVSIPPNRGDICACDGRVLATSVPYYEVGIDPNSNGIEKEVFDKEIDSLCIC
                     LANLLPVKTREQYFNEITHSRDNGNRYYRINGKVTYDQLKAIKTFPILRYGKIEGGLI
                     INEYTERELPFGMLAKRTIGKVDSDKRKGVGLEMAYENDLRGVEGKTVKRKIAGNQWM
                     PIRDANEVAPKDGYDLITTIDINIQDVTETALERKLRYHNAHHGCAIVMEVSTGKIRA
                     MANLTRDENGNYYESFNYAIGEARDPGSTFKLASMIVALEDGCVSPTDTIDTEDGTHN
                     FHGKEMKDSHEGGYGKITIQEAFELSSNVGISKMIYENFYHRKSDFVDKLYEMRLNQP
                     LGVEISGEAIPTIRRPEQGWSKISLTQMSIGYELLMTPLQMLTFYNAVANDGKMVKPR
                     FAECLTYRGHIVKNFQTEVLVEKICSDKTLQAVQDMLAGVVLRGTAKNLINDNYSIAG
                     KTGTAQTDYGKPGGPLQYQASFVGYFPAENPKYSCIVVINQPDISTGYYANSVAGPVF
                     KEIADRIHAMDTIFSVKNDMKYDTIIPMAKSGYKSEIQKVYFLLGFDKKQSVVTKGEW
                     AKPFVENRAVKYYPRNYTNKNLVPDVRGMGLRDAMMVLEEFGLKVVVEGRGKVIQQEP
                     QPNQSFIKGQQIKIILG"
     gene            complement(11026..11400)
                     /locus_tag="PLK75_01075"
     CDS             complement(11026..11400)
                     /locus_tag="PLK75_01075"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOY37867.1"
                     /translation="MAKTAENKKKKGQVRKTLQGLMSGSILATDFVRRQFPFLVMLAV
                     LALIYIANRYHAERVFIESEKLKKEIVELRSEKIATQSVLMRKSRRGEVMRMLKENGS
                     ELNESSIPPMKIFYKSEKKKNK"
     gene            complement(11403..12299)
                     /gene="rsmH"
                     /locus_tag="PLK75_01080"
     CDS             complement(11403..12299)
                     /gene="rsmH"
                     /locus_tag="PLK75_01080"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOY37868.1"
                     /translation="MYHQPVMLNESIAGLKIRPEGIYVDATFGGGGHSRRILEEIGEN
                     GILYAIDQDEDAAQNLPDDKRLVFIHGNFRYLQNYLDYFGIDKVDGIIADLGVSSHHF
                     DEECRGFSFRSESKIDMRMNGKAVKDAAYVLNNYGEKELATIFRIYGEIDFAAKLARD
                     IVANRDAMDMSSTSVVVSLAEKYIPPVRRNKLLAQVFQALRIEVNDEVNALADFLNTC
                     TGVVAKEGRLVVISYHSLEDRLVKNVIRSGNIEGVEQHDLYGKIEGPWEPVTKKPIAA
                     SEEEINMNSKSRAAKLRIATRK"
     gene            complement(12305..12772)
                     /locus_tag="PLK75_01085"
     CDS             complement(12305..12772)
                     /locus_tag="PLK75_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mraZ"
                     /protein_id="HOY37869.1"
                     /translation="MFVGEYSCKLDNKGRVLFPAALKRQLPADQQDRFVLKRDVYEEC
                     LVLYPILEWEKQNRAIRKKLNPFNREHALFLREFHRETAEILVDSSNRLLIPKRLLEC
                     LGENQTELVFAGQDGKIEIWSSKMYYSKKTGESDFANLAERILGGDFFYQNED"
     gene            12951..13787
                     /locus_tag="PLK75_01090"
     CDS             12951..13787
                     /locus_tag="PLK75_01090"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HOY37870.1"
                     /translation="MSEKDVLKIDVEKVIDNKNPKLRKKLPKFLINYLKKIVHQEELN
                     EFFKESHGKTGLDFVDIILSYYNIGIDAEGVENIPDKGRFVFAANHPIGGIESIAFIK
                     VVSEKYKELKFPVNDILMELKPMASIFIPINKHGGQSKFAIKELEDYFAGNGQILYFP
                     AGMCSRRIKGRIQDPEWKKTFITKAIQHKRDIIPVYIAGRNSNFFYNLSNFRTFARIK
                     TNLEMLYLVDETFRQFNSTIRLKFGKPISYTHFNDSQTHQQWAENLKEIVYQLGGIQL
                     KH"
     gene            13866..14846
                     /locus_tag="PLK75_01095"
     CDS             13866..14846
                     /locus_tag="PLK75_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY37871.1"
                     /translation="MESVIPPVDRKLLLKELADGKLLRESNMGKTEIYVFSHEDSPNL
                     MKELGRVREITFRDAGGGTGLSCDIDTYDISETPYLQLIVWDPEELEIIGGYRFIHGA
                     SVLDNPIEKLATSPLFSFSDLFLKEYLPYTIELGRSFVQPKYQQGNQARKGLFALDNL
                     WDGIGALTIEYPDIKYFFGKVTMYKSYDVEARNHLLAFMNLYFPDKQHLVSPIAPIEV
                     DFKKYQKAFRGNSYRNDYKTLSNIIRKSGERIPPLINTYMNISSSMKTFGTVDNPHFG
                     AVEETGILVNISDIYSSKKDRHILTYKRISKLSSIISSKLKMKVKQKRKL"
     gene            complement(14841..15170)
                     /locus_tag="PLK75_01100"
     CDS             complement(14841..15170)
                     /locus_tag="PLK75_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37872.1"
                     /translation="MKIVISEKTTDMSHLMELLRSIEGVKGLTRFGRNSVRVSGSKYE
                     GARVFLKKNRIVILADFSSGASHRAFNLIIFIGGIIIPYMIYGIFFYPRIYRFKKHIR
                     NNLIIML"
     gene            15256..16695
                     /locus_tag="PLK75_01105"
     CDS             15256..16695
                     /locus_tag="PLK75_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY37873.1"
                     /translation="MKKVALFILVIIGFSVLNAQNLVPNASFENAWTCPSYFYTEAVG
                     DIIPMWHNPSRGTPDYFNRCSDSIVSVPLNFAGTLEAHDGNGYMGLILRETFEPELRR
                     DGVSREYIQVKIASALKAGHLYCFTMQYALSSRSPFAVDALGVAITKTKISSRDARQI
                     IQMPQITNIPGHFMQNKTGWAELCGIYRARGNEQYLTIGNFLNDDKTSFIPMNDADAD
                     SNFVYAYYYIDNVKLIEIEDEFQCGCQDDFSVGSDYLSEKYDRKTGFNTRKPDNSDNN
                     GNLADNRNGGNTTLINGNAVDNGNNPSGNNGNNAENAGDSDKNYSNIGNNSDENKGTD
                     VSGNYTAGNSNENLSDNTKDLDNFDIASAGIGDAFRMSRIYFEFNSAELLDPSFDQMN
                     KLLVLLKENPRLRIEIRGHTDNVGTKQYNRKLSIARAAAVYNYLIDAGIEKTRMKYRG
                     FGNDVPVATNDTEEGRQLNRRVEILIVEL"
     gene            16706..18493
                     /locus_tag="PLK75_01110"
     CDS             16706..18493
                     /locus_tag="PLK75_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOY37874.1"
                     /translation="MKFSRSISFKISLLVVCLVVLSIFMIALFSYIQTSKAIIDRTFG
                     QLNSVKWEKSRRIENFFGERTNELKLFASSKPVAQLCELMESENLKTGENLKTSYENE
                     AYFSNYLLLHPDILKLFIINNNNRLIETSAQQRVFISASPSDPLKYISDTIFNSTFSQ
                     PVYYTDIIQDSANNPVLFVLLPLDVPGNKFIIGFVINASAISDIMYENNPFNGMGKSG
                     EAYLVGSDTLMRSQSRFVPNSVLKLKAKTDAVINALGGNDNTGIINDYRGIKVLSSYG
                     IISINNIRWAIMAEIDYKEAIIPIDDYRNNMIFLGMLSALLFTAFIVFVTHTIIKPVV
                     KLKNAALEIAKGNYDTVIENHSSDEVGQLTLAFNRMAQQLDEQRKNLEKERISRLSEM
                     IDWQENERQRLSRELHDSLGQVLLSIKMRIQRAKGKTDNDNAIIDETNMLLAETVQEV
                     RNISNDLMPSVLISYGLQGCLKNLCENILARADIEADFKTNIESLLPSKIQTYLYRII
                     QEAVNNIIKHSKATRVSIVLMNYDSGIELTVSDNGCGFGQDILSQNRGQGLRNITERV
                     ELLGGSCTIDSHNGQGTQIFVNIPNKPHE"
     gene            18486..19139
                     /locus_tag="PLK75_01115"
     CDS             18486..19139
                     /locus_tag="PLK75_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY37875.1"
                     /translation="MNKPISIFLLDDHKLFRDGIRAVLSDEPNISITGEAGSSVELFR
                     LLENTHPDILIADIELPGMSGIEIAANLHQTRPEIKVLVISMHQNEEYVMNACRAGAM
                     GFLPKETGKEELVTAINRIAGGDTYYNADITGKIIRKISDHTRNTEVNPLTELSKREI
                     EILKFFAEGYSNLEIAEKLFISIRTVESHKNHIMQKLMVKSQVDLVKIAIRYKLVNL"
     gene            19272..20048
                     /locus_tag="PLK75_01120"
     CDS             19272..20048
                     /locus_tag="PLK75_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37876.1"
                     /translation="MKKLFLSLVVVSMTIMFNITAVNAQESPFHVGVDIWSRYVWRGT
                     DFGSSPSVQPCIEYSTHGFTIGSWGAYTTSLNAPAQEADLYLAYTFANDMFKVIVTDY
                     FFPTDGGLNQYFNYKKGETGHYFEGGIAFNGTEKLPLKFFAGTMFYGNDLDYAGDNRF
                     STYLELSYNPTIKGTDFSVFLGANLTAASDDDLNYIDPVTLQPAPVSGFYGDKAGICR
                     VGISAAKKIQITDSFELPLAVMLQSNPMKGNLFLTAGFTF"
     gene            20086..21321
                     /locus_tag="PLK75_01125"
     CDS             20086..21321
                     /locus_tag="PLK75_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOY37877.1"
                     /translation="MILNAIFDSGTTTFMILCTSLVMLMTPGLAFFYGGMAGKRNILG
                     IMMQTFVSLGLTTVMWIGFGYSLCFSGGQGGVIGNFDMAFLSNVPFDQAFDPINGKNY
                     PTYIFVAYQMMFAIITPALITGAFVNRVTFKSYLIFLVLWQVFVYYPFVHMVWGGGLL
                     AEWGVKDFAGGIVVHCTAGFAALASVFYVGKRRDRQSPPNSIPLVAIGTGLLWFGWYG
                     FNAGSELTVDQVTTLAFLNTDTAASFAAVTWLAIEWIHTGKPKFIGLLTGAVAGLATI
                     TPAAGFVPLWAAVLIGISAGAVCYLAVSLKNKLGWDDALDVWGVHGIGGLLGTVLLGV
                     FAMKSFGGQSGLIEGDYAFFGKELVSIVIAAAYAFIFTYLMLTIINFITKVRVSEEQE
                     VVGLDESLHGEKAYDEGAL"
     gene            21457..23775
                     /locus_tag="PLK75_01130"
     CDS             21457..23775
                     /locus_tag="PLK75_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOY37878.1"
                     /translation="MLGKQVSHGHTTVATGLILLLLAWSCSQVKHVPADKYLLANVKI
                     KCNNSAIDPDDLESTLKQKPNRQTFGIFKFHLLMYNIAKTGKERKWKNKIANVVGEPP
                     VVYDETLRKRTMQNINYFLNYKGYYHATVSDTVKFKRKKARLTININTGKPHIINNIS
                     YDIQDTEVKRIVEADVTSCLLLTGAPLDFDVIEAEQARLVKLMKKNGYYDFTINNIHF
                     YADTNSVPLNADITIAIRHSFTGENDTTGKFEKYRIGEVYIFTEYDQKKYLLERDKYL
                     HSLILSEFEGYHFYSPGNLKIRPLAIIQTMFIKKGDYYDISNIEKTHSHLSGLKQFKL
                     INIDLVADSADSKTLNCYIYLTSLNKQSYSVEIEGTNSSGNLGAAGIITYQNRNLFKG
                     AENLSVKGSLALQTLTQVEGIENRFLNTFETSGEISLNVPKLILPFYRNDEFVKNKGP
                     KTQFSVSTSYQKRPDYTRLLSNATIGYYWKGGENNYLTHFFNPIELYQVRIWDFNPEF
                     MAGIENLFIRYSYENQFISVISYDLLYTNQNLNKLKNFWYLWWNVETAGNLMEGIFEL
                     SGQTPVEGSYQLFGTEFAQFVKTDIDLRFYQVFSKKHSLVYRAYFGIGIPYGNSKSNG
                     LPFIKKYFTGGANDIRAWQVRTLGPGSYNSTSLINQMADMKLMLNAEYRFDIASFLEG
                     AIFLDAGNIWAIDKDDDREGALFTPGTFYKEIALGTGIGARFDFSFFIIRFDFGVPLY
                     DPKYPIGERWLNTFSTFEFSDLTFNFGIGYPF"
     gene            23859..24752
                     /locus_tag="PLK75_01135"
     CDS             23859..24752
                     /locus_tag="PLK75_01135"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY37879.1"
                     /translation="MIKKLSVFIFLCIGISSFPRTITDTSETVILKVLSWNIQMLPNG
                     FDIFSESLRKLQHVRVDWIIEHCLAQDYDIIVFQEVFDVQMKHKIRKHLKSEYPFQVN
                     TRTKFGRFTSNGILIVSKLPIKYIDHVIYNRGVHADAWASKGCTLVEGEKDGHKFQLA
                     GTHLQAGGSKKAKFHRRSQYKDIAHLIEKNKKDRIPVLVAGDMNTGNDETEDYRHMLE
                     VMKVFDFPVNDTCPFTIDSNNSWNFNSRSRQLDYILVQARNTVTRIYNQYILRLTRDF
                     SGKATDYSDHYGIVAEIELGN"
     gene            complement(24773..26272)
                     /gene="glpK"
                     /locus_tag="PLK75_01140"
     CDS             complement(24773..26272)
                     /gene="glpK"
                     /locus_tag="PLK75_01140"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOY37880.1"
                     /translation="MGDNKTYILSFDQGTTSSRTVLFDERFRIVGIEQQEIQQFYPRP
                     GWVEQDPVEILEAQLFVLRKLIHRLGIEPSSIASLGITNQRETTVVWNRHSGKPVYNA
                     IIWQDRRTADYCNYLKSIGMEEIIKQKTGLVIDSYFSATKLRWILESVDGVRKSAENG
                     DLLFGTIDTWLIWNLTGGKRHITDVSNASRTMMFDINKLCWDNELLDIFDIPSAMLPE
                     VVASSGTFGIAKTGLTGKGNIEIGGIAGDQQAALFGQACFDEGMVKNTYGTGCFMLLN
                     TGSKPCNSQNGLLSTVAWKVNDSVVYAAEGSVFIAGAAVKWLRDGIHLIRDAAETNTI
                     SQSLSDSGDIFVVPAFAGLGAPYWDMKARGAILGITQGVTEKHIVRATLESLAYQTRD
                     VLEAMQQSAGVTIKILKADGGASANNYLMQFQSDMLQIDVVRPEIIESTALGAAYLAA
                     ISAGMCDLKSISEMWQQAHVFSPSMPEEKREALYRKWKKAVSRCMNWED"
     gene            complement(26262..27194)
                     /locus_tag="PLK75_01145"
     CDS             complement(26262..27194)
                     /locus_tag="PLK75_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY37881.1"
                     /translation="MSYEKKSFTTSDGIAVFYHKWIPDTSYIRASIVIAHGMAEHGFR
                     YCNFASALNANGFVVYAPDHRGHGATAGSPEKTGYFADTKGWCKVTSDIREMICIARA
                     ENPGKPSYLLGHSMGSFIARTYICLHGNQIDGVILSGTGQNPSLILYAGSFIAWIIGK
                     IKGKKHRSRFLDNLSFGSFNKGYSTPFQWLSRDSAIVEQYIADPYCGQVFTAGFFSDL
                     FHGLLFIGKRKNLDNMPKQLPVFFISGDNDPVGAKGKGVSKVHKAFLDCGMTDVRFTL
                     YEGARHEVLNETNRNEVYDDVIKWLRRKILYHGR"
     gene            complement(27201..28307)
                     /locus_tag="PLK75_01150"
     CDS             complement(27201..28307)
                     /locus_tag="PLK75_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOY37882.1"
                     /translation="MEEDVVFAGFDTLQMFMKECLMAAGVPADDAETVSDVLIEADKF
                     GIDSHGINRLKPIYIDRIRDGILNPVTKIDVVKDTFTTAVLDANNGMGHVVSKAAMKM
                     AIEKARVYGMGMVAVRNSSHYGIAGYYGMMAAAENMIGITGTNARPSIAPTFGIDNML
                     GTNPLTFVVPSDEDFPFFLDCATSVSQRGKIEFYAKNGKQLPKGWVIDRSGESKTDPA
                     EVLTDLTKGNAALTPLGGIGEETGGYKGYGYATVVEILSAALQGGVFMNALTGFDNGK
                     KVPYRLGHFFIAINIGHFTEVSEFKSTTGNILRELRNSAKMPGAERIYTAGEKEYIHL
                     QERSLSGVPLNKAMRMEIRALAEMFKLSGYEHLY"
     gene            28463..30103
                     /locus_tag="PLK75_01155"
     CDS             28463..30103
                     /locus_tag="PLK75_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37883.1"
                     /translation="MYTSTAQSGDALLDKYAHYRARLFDDFVVVSPDVEDFGVNIPAT
                     DRVLDTLGKPVYISWGDGNCNFNHYLGFLATEYRLLKNNGRDYQETYEMLIYALLAIE
                     RLDLYSEYTLRLHENKSKLVDGDTIREYIEYPSDINGFLIRDDVSLGFWYRYSTHFNI
                     GFGKINKTKDGTNTYQSIFKMGVVPKEEMSQDNIIRMLHALAMVKMLVDDEPQYMVSV
                     NFINTLIPEYLASKQILADDSIYISRWVEDLANRLIGQMQHPYPEKSLCLKPCCGMAK
                     VSSNHFLSLISTPWYITNPVTNELVAEGSGEDMGLWINSYGVAEAGNAITSTQNHHLG
                     KSAYGVNPYLFKALLFKQMRLLPGGSFPLPKSIDDYMLRDLAVIADINWNRKSKNLFY
                     ALRDKRKRLTYEHQLLILNILHPEKYKSLYSPGTRYFEEDKLYYRSLLEAAPAAGPTS
                     VQSNPEYSELWNSSSRMIWPGKGAPDPTNRWDFAGLDYLYLHNLYRLVFCPDNYSLPE
                     NRMKPQDKKTYLNRNSAAPGYDAEFFFSAPKAVRHKKE"
     gene            30198..32153
                     /locus_tag="PLK75_01160"
     CDS             30198..32153
                     /locus_tag="PLK75_01160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY37884.1"
                     /translation="MKLSVIIVNYNVKHFLEQCLHSVFSAIGSIDAEVTVVDNNSVDG
                     SCALVKDKFPTVKLITNKKNYGFSYANNQAIKQAQGEYILLLNPDTVVQENTFTAVIG
                     FMDSHSDAGGLGVKMIDGKGRFLPESKRGLPTPAVAFYKIFGLSKLFPKSERFGRYHL
                     SNLDKEKIHKVDVLSGAFMLLRHSVLKTIGLLDEDYFMYGEDIDLSYRITKAGYNNYY
                     FPETTIIHYKGESTKKGSINYVMVFYKAMIIFARKHFNRKNARMFSFLINMAIYFRAF
                     LAIAHRFLLRLVAPALDAILIFAGYYFLTPQWSLLKFGEIDYYPPEFFHYAVPAYIIT
                     WLITLLFTGGYDRPLRVINIFKGLAIGSVIILLYYSLLPEHLRFSRALILLGTAYAAI
                     AIPLMRALLHFTGIRAFRIDMQKLKKVVIAGNKEEIVRVSALLNQTGLNNQVVGFVST
                     MQTETNGFFMGSIAQIDEIVRIYKINEVIFCAKDFPSQQIIKYMLLLAEQKIDYKIAG
                     PDGVTVIGSNSVHTAGDIYSINLNSIGSPKNKRKKRLFDVAMSIAIALIAPILLFLVK
                     SPMRLLKNLIFCFFGLKSFVGYYKNAGVAGLPYLKPGILKPLDPGVETVSQQTAESAN
                     ILYARDYNVLRDFRIIIRSLNKLDREA"
     gene            32143..32676
                     /locus_tag="PLK75_01165"
     CDS             32143..32676
                     /locus_tag="PLK75_01165"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOY37885.1"
                     /translation="MKLENNRIRLRAPEPEDLELLYHWENDTGMWVVSETLEPFSRFQ
                     LKEYISQCNRDIYTAKEQRLMIETIESPHCVGIIDMFQLDPFHRKAALGILIGNEYRK
                     QHYASDALEIYCGYAFGYLQLHQLYCEIIASNTVSIQLFEKSGFRCVARLKDWRRIGS
                     DYTDVFLYQRLQPDNNR"
     gene            complement(32684..33172)
                     /gene="ispF"
                     /locus_tag="PLK75_01170"
     CDS             complement(32684..33172)
                     /gene="ispF"
                     /locus_tag="PLK75_01170"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOY37886.1"
                     /translation="MHNVRVGFGYDVHQLAPGLTLTLGGVNIPHSKGCVAHSDGDVLI
                     HAVCDALLGAAGKRDIGFHFPDTDSKYKGIDSRHLLAEVYNIITEAGYETGNIDVTVC
                     LQKPKIAQYTDDMCKVLASEMHVDIGCISIKATTTEKLGFVGREEGIAVYAVALIFKR
                     EI"
     gene            complement(33184..34446)
                     /gene="porV"
                     /locus_tag="PLK75_01175"
     CDS             complement(33184..34446)
                     /gene="porV"
                     /locus_tag="PLK75_01175"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOY37887.1"
                     /translation="MKKVRFFILLTSVFALNYSYAQNEPDVDIWGRTDNVPIITTAVP
                     FLLIAPDSRAAGMGDAGVAVTPDANSLHWNAAKYAFCEKELGVSLSYTPWLRQLVPDM
                     NLAYLSAYKQIDAENTIAASLRYFSLGGITFTDINGGTIRDFTPNEFSIDFAYSRLLG
                     KNFSGAVALRYIHSNLTGGVSNNSGEDTHAGNSGAADVSFFYNSENIDLGNMKTNLMW
                     GLNISNIGAKISYTSNEKRNFLPANFRTGVAYLMNINEYNSIVVAVDLNKLLVPSPPS
                     YLLDSTGANVYDNNGNIVIAYGKNPEISVPAAIFQSWYDAAGIAEPFRKDETTPNVFK
                     EELSEIAWSFGMEYWYNKRFALRAGYFNEHQYKGNRKYFSVGAGLRLNVFGLDFSYLI
                     PTAQQHPLQNTLRFTLTFDIDGLSDENN"
     gene            complement(34541..38362)
                     /gene="porU"
                     /locus_tag="PLK75_01180"
     CDS             complement(34541..38362)
                     /gene="porU"
                     /locus_tag="PLK75_01180"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOY37888.1"
                     /translation="MLPRMSYKLLLLSVIGLLIGVYAFSQSVEFSDSIRWNNPIFSLN
                     NENKPQIILSFSKSGQKNEHFLPVYYKKIRTQSEISVKNPKIVNAVYEALPDSVLNFL
                     TDVDKINEEINIEVFTSCFRKEYAVSFELIPLRRNKITGRIEKLVSFTLQTTAVAKPE
                     LRNMRSYASSSKLSSGKWYKIKLDASGIYKLTYEQLTQMGFSNFSTLGVFGFGKMLPK
                     KNNEVRYDDLPERDMMKVDANANSVFDAGDYILFYADGPDRIYYDNSSQSFKHEKHDY
                     SEYAYYFISDRGTWKSPQVINSEATFNAVVNSYNEVKFLEKDSLNVRGMGRTWFWRHF
                     DFYTSYQFTQNFPNLITSEPLDIDIYLAARSSLSSNFKIYIANTLVNTTLIGYVSGNY
                     SSTYAKEEIASISHLAAGPDISYRIDYIKSTSDSEGWLNYIRASGRCSLTMSSDFLIF
                     RDINSVDPGSIAQFVLGNTNANTVVWDITDPVNAVQINPVLAGTQTKFNRNSETLHEY
                     IAFNPSGSFQSPVTEGDGLGLIANQNLHALANPDLIIVTYPGFRAQAQQLADLHTLND
                     GMSCIVVDPAIIYNEFSSGCPDVSAIRDFVKMFYDRAGSDDEIPENLLLFGDGSFDNK
                     APSGVNGNFVPTYQTTNSFSPSGAFVSDDFYVMLDPNEGTVNGYDDSDIGIGRIPAKS
                     VSEAQTVVNKIIAYISSAYKGSWRNTVTFVGDDAEDYDIHQIHCNEIADIVRANYPVY
                     NVDKIFLDSYEQVSTVQGMRYPEVNEAIDNRIQKGTLIFNYTGHGNTKTLAHEVVVDL
                     SQINSWVNNNNYAIFFTATCEFSRFDDFELVSAGEQVLLNPHGGGIALFTTSRLVQAG
                     ANTSLNKAFYNYFFVKDADLKPYTMGQIIIRTKNELNSDTNKRNFVLLGDPAMRPALP
                     QYNVVTTSINGINVSSFADTVGARSKVIVTGYIQDEDGNKMSGFNGLLFPVVYDKNME
                     FQTRGNDGKPPLEFSEQMNILFKGQTSVVNGEFTFSFIVPVDIAYFYGNGKISYYAHD
                     NNVDAHGNYSEIMIGGSSDSNIADNEGPEIELYMNNEDFIIGGITDENPNLLAFVSDS
                     SGINTVGSGIGHDILAVLDENTSHSIVLNEYYESNLDDYTSGKILYPFSNLELGPHTL
                     SLKVWDVLNNSSVAYTDFMVASSSELVIDHVFNYPNPFSTNTQFYFEHNQPNVPMDVL
                     IQVFTVAGKLVKTIEVVVVSDGFRSQPISWNALDDYGDKIGKGVYIYKISVKAPNGNI
                     ARQIEKLFIIN"
     gene            38494..39480
                     /locus_tag="PLK75_01185"
     CDS             38494..39480
                     /locus_tag="PLK75_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY37889.1"
                     /translation="MIRRILFILSILLTGIYGAVLGQDAHFSQFYANPLHLNPAFTGS
                     ERCPRVSLGYRNQWPALGPTYVTYSASYDQHVNFMQGGLGLHILSDVQGDGTIKTNII
                     SGMYSYTLPVSRNFAIAGGFQASFVMKTIKWDFIFPDMIHPLYGPIYTTQENPDLVND
                     NKNYVDFSVGLIGFTERTFFGVAVHHLTEPSESFLRGSDAVLPRKFTAHFGTDIPINN
                     KKFRRGELSLSPQLLFHQQGQFQQFNWGIYVNRKAIVAGFWLRQNFNFQYDSFIMLVG
                     FRQDNIRFAYSYDFTVSRLRNSTYGSHEVSFAYIFPCHEKAAKKRVISCPSF"
     gene            39516..41504
                     /locus_tag="PLK75_01190"
     CDS             39516..41504
                     /locus_tag="PLK75_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY37890.1"
                     /translation="MKTNLFTWSFIILLMVAFVSCKKEVSNTTGRNYNDPKHGGFENH
                     PEYEQETGPGLVFIEGGSYAMGRTEQDVIFDWNNAPRRVTVSSFYMDETEIRNIDYLE
                     YLYWLRYVYGQEKYFIYNEALPDTLVWRQRLAFNEPMVELYLRHPAYEDYPVVGVSWV
                     QASKYSDWRTDRVNEVILVREGIFVDNLVPRQGVNQFNLDSYLFSSSSYDITLELPVS
                     KDNKNKGNTNHDGKVDNLYRPADYFDTDNKNKQQQNVTETRRNVRTEDGIFLPRYRLP
                     TEAEWEFAALAHIGNTYNERIYNRRLYPWNGHYIRNDSRKGDERGQIMANSMRGRGDN
                     MGVAGALNDKADISAPVKSYWPNEYGLYCMAGNVNEWVMDVYRDMTYDDMEEFRPYRG
                     NVYDDYNITIDPENGEVTLTPEASNYFNYVGQIQRKPVSDEACFNRENYTTADNRNYL
                     DGDLESLIPGGAANYWNSTVDYKPDTLKVHNPIDSIDLTKLPKGSSYRKTGNDIEIFL
                     PNKIVIRVDQNNVPTYTLPNGYRITQDSGKQVVILPDGSKVLPGGVIEFTDGERLLPR
                     DIFAYTGYMYKDGNWGRDGEWGAGQYDKMNPKGNKGDNMKSLTTDRSRVFKGGSWNDR
                     AYWMVPANRRFLDEKMARADLGFRCAMVRVGSPVQGKK"
     gene            41557..42849
                     /gene="murF"
                     /locus_tag="PLK75_01195"
     CDS             41557..42849
                     /gene="murF"
                     /locus_tag="PLK75_01195"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOY37891.1"
                     /translation="MVTTEQLYKIFLKTRSVCTDSRRVTPGSVFFALSGENFDGNNFA
                     AQAIEMGCAYAVVDKPAVALNDKYLVVNNVLQALQDLARHHRKQLRIPVIAITGTNGK
                     TTTKELISAILSKKFRVNATRGNMNNHIGVPLTILSMNHTTDMGVVEMGANHPGEIAL
                     LCDIAQPNYGLITNIGKAHLEGFGSVEAIIKTKAELYDYLMGHEGIIFLNNENPILKK
                     LVGSYPSVNYGVSDESFCYGKITYKEIYVGVDWICGQNTGLAYSFLIGDYNFENIISA
                     IAIGNYFNIAGSIIDKTVSTYTPSNNRSQWIKGRRNQLILDYYNANPTSMMAAITNFN
                     NYDSEKKCLILGDMLELGDVSEKEHQNIIEYINAYPYSDVFLVGNTFGRFKDSYNFHF
                     FETTEMLAGYLSKNPPTGYLILLKGSRGMKLEKCVDYL"
     gene            42954..43892
                     /locus_tag="PLK75_01200"
     CDS             42954..43892
                     /locus_tag="PLK75_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HOY37892.1"
                     /translation="MKNFLLSGVLLVFCFVAAAQTTLPNMNFEDWQENHYSSWYPSNF
                     YWECLPTTIWSSGNAASTQVNIFPTYRTTDAQSGSYAACLETKEIFTQTASGSLFTGW
                     FEPAMLSSEAHFGVPFTGKPTAFRGWYKYTPANQSGGTIDTCSIYAVLSRWNGSERVE
                     IARAELYTYTTVSSYTYFDLPFNYYNSLIPDTIAVIFSSSKHGDIFQGGIGSKLYIDN
                     IDLYYDLSSVQPNHAGVNEVHILFDSYGKNISVERNYVKPASIELFDMTGKKLYSYSI
                     EGLKREMNFDGLRTGIYVCRYSDNAGLVKTVKIYFK"
     gene            43897..44637
                     /locus_tag="PLK75_01205"
     CDS             43897..44637
                     /locus_tag="PLK75_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY37893.1"
                     /translation="MQKITLLAVLVYLSASAFSQTISFGPKLGMVAGSPVPYSFIPEG
                     AKGAPKPGRNVGLFVKFDFTENYSLIYEVSMTRKRTTFETPLDSVAYVDRIQHPLFPD
                     VVFEVETFFNGTAVGCFDNYYLEQSLTHQMRITEKMNGLFGLYTAWLQKSNTYAKGIG
                     RVGFSPDIIEQELDYSSKMRQWDYGLKAGIGYEASKHMDIDLKLSYGFESVFCDSFSM
                     LNYTVNNTFVELSLNYKFFILRQLNSQI"
     assembly_gap    44695..44794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(45005..46345)
                     /gene="trkA"
                     /locus_tag="PLK75_01210"
     CDS             complement(45005..46345)
                     /gene="trkA"
                     /locus_tag="PLK75_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOY37894.1"
                     /translation="MKIIIAGAGEVGAYLAKMLVKEDHDIILIDNNEKRLKEVESDAD
                     LLTVTGSCTSIRTLIEAGVKTADLFIAVTVTEQNNTIAAILAKKLGARKTIARIDSME
                     YLEPKYKPHLLGLGIDKLIYPEYIAAKEVNGLIKQTGTSEIFDFSGGKLTLFVVKLDS
                     DAPIVNKTLIEADAMTKSTDYRVVAISRNNKTVIPNGNDVLMVNDLVYVITNPSGIVS
                     ILKYAGKKKLEISNIMILGGSRIGRMVAMRMQSHLNIKMVDIDSDKCEMLANELNKCL
                     IINGDGRSLEFLEEQGIQDMDAFLAVTGNSETNILSCIMAKRMGVKKTIAEVENIDFI
                     DVATQMGIDTIVNKKMSAASYIYSSTLNAEVSSVKCITGSDAEVLEFVVQPGAKITGD
                     IVRNINFPKKAIIGGVVRDDKSFIANGNTLIKANDKVVVFALPEAILQIEDFFN"
     gene            complement(46525..48636)
                     /locus_tag="PLK75_01215"
     CDS             complement(46525..48636)
                     /locus_tag="PLK75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOY37895.1"
                     /translation="MNFLKQYPFVRLLIPFIAGIILAGYFTIPCIFGIVAITVLLLVL
                     IALYALKEKPPESVFTFTIFLFICLSALTLTGLKSETANDSRLSGYKGQIIGKVSSDP
                     QVSAKTVKLDIALEAVKMVDTWYEGQGSIMVFVPADSAAMLLGVGDKLILSPELKQFE
                     NQGNPEEFDYKKYMWYQYIQYTDYIKSDEWKKIEEPPSGSLFVLAANIRNSVTESFRK
                     CGLSGDELAVATALTLGNKSYLSDEIRQSYSVSGGMHVLAVSGLHVGVVYLVISQLLG
                     FIKSKRRQWIKTLLIILLIWGYALITGLSPSVVRAALMFSLFASAGLFRKNANSFNIL
                     AAAAFFSLLADPLVLHQIGFQLSYAAVFSILYFYPKIYGLVEVRSRLADKVWSLLSVS
                     VAAQLGTIPFTLFYFNQFPSYFLLTNLIIIPLVTVAIYLAIAIAMFSWIGFLGSALGW
                     VFSKVIFLMNSSVSFIESLPGSVIDGIYISNTQFVILLFLIALVVLFLEFRRSGYLFA
                     TLSLIVVFVAIGITKSLHTINQRKIIVYNLRNTTAINIIDGRDNILFLGLDPISEKSR
                     KYTMANYWNTIGAGKEKTICLQDMLAPRLLSNIIQIGNRRVFVKNNFIGFYDTKIYIP
                     GATYAKSPETLNDIAVDLVIITDEASCDVYELSRQLKFDQLVIDASVSDNRRKLLLQQ
                     AADIGLSAFDVKNKGAFIIDK"
     gene            complement(48646..49182)
                     /locus_tag="PLK75_01220"
     CDS             complement(48646..49182)
                     /locus_tag="PLK75_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY37896.1"
                     /translation="MKNTYIILAVMVMLVVSCKPKNKPEQSSEALRAELSADIDKTEK
                     ELYVSKNFDTQKAKQMIGKYVNYSRKFPNDTTSAKYLIKASEIALSLNQPHNAVEYLT
                     TVEKSGMQCNDYPLAIFYLGYTYHNYLNDTAKARHYYELFIQKYPDHSLSDDAEASIK
                     FLGLSDEELIDAFEKMNK"
     gene            complement(49257..49808)
                     /gene="def"
                     /locus_tag="PLK75_01225"
     CDS             complement(49257..49808)
                     /gene="def"
                     /locus_tag="PLK75_01225"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOY37897.1"
                     /translation="MIYPIYVIGHPVLRRKTELVVKNSPEIQKFIDDMFETMAESDGV
                     GLAAPQVGKSLRIFVVDTTPVAKDNVELMNFKRVFINPQIVEKSTETVLWEEGCLSIP
                     GIHENVKRYTRLTMEYYDRDFNFVREELDGFKAIVVQHEYDHLDGVLFTDKISPIRKK
                     FLKNKILNISKGKVSTFYKIVTP"
     gene            complement(49805..50230)
                     /gene="ruvX"
                     /locus_tag="PLK75_01230"
     CDS             complement(49805..50230)
                     /gene="ruvX"
                     /locus_tag="PLK75_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOY37898.1"
                     /translation="MPRWIALDIGKKRTGVAVTDKLGIIATALGFTETGKLSGFLQDY
                     LAKESVDLIIVGYPRNMNNLPSDAVKYIEPVVTRLKKLFADVHFVYYDERFTSQISFQ
                     AMIDGGVKKMKRRNKGLVDQISAVLILQAYMDYVKNTKL"
     gene            50315..52687
                     /locus_tag="PLK75_01235"
     CDS             50315..52687
                     /locus_tag="PLK75_01235"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY37899.1"
                     /translation="MNKILLFPTFLLASILLSNVSFTQTTISTYTNVPLQALIADTLV
                     NGCTQVSNITVNSNGAWGYFRKNNSQFPFSSGVIIASGAISNAQGPNSSSSMGNSINS
                     GSDPDLVEIAGGGSIYDCTKIEFNFIPADDTVEFRYVFGSEEFPEYANSNFNDVFGFF
                     LSGPGISGPYLNNAINIALLPNGQTVTINNVHNYNYYTATPGSQSNSAGSYYGAVQYD
                     GNTIVLTARAIVQPCQTYHIKLAVGDRGDSAYDSGVFFEANSFVSGTMIEGINHSQFG
                     GESDLWEGCENYYVISRAPGSSSALPVTVNLSVGGNSTATAGVDYTTLPTVVTIPAGQ
                     MSDTIFYNAFNDAIEEGHETIILNFYTTCPCGNMPSVIADTIWIYDAQKIKGGIQDVQ
                     TNYCGVAPPSSLTLYASCNLDTSQVSNVYYHWSTGSNTNSTTITPQPGATTYYVTMSD
                     DCGNEVFDEITILVSSMQISNQVTTPVTCHDACNGTYVVNCVNNFPPFTYTLVNADYV
                     WFPDSIHSQTTGSFNHLCPGTYLVTITDDIGCQITTEFTLNNLPPINYSVGITTGDQN
                     YCTSPGPITLTAEANQSSATFEWFNGSGNSSISVTPTVGVNNYWVRIYDACQNMFEDN
                     VTITLSQMFATTSSTDDTGDCEGTAWVTVSNGISPYSFYWPDIDEFGYLQEDLCHGTY
                     AVNITDNIGCQLTKQATVLLNVSVEETGLTQNVIYPNPNSGSFVVNMSDFGNNIYEIV
                     IFDVLGKCIYKANTENGKNVYIRNLAPGTYYLNVTSASEIKFSEKLVIIE"
     gene            52786..54513
                     /locus_tag="PLK75_01240"
     CDS             52786..54513
                     /locus_tag="PLK75_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOY37900.1"
                     /translation="MAKEIKFSLVYRDMWQSSGKYQPRLDQLLKIAPVIIDMGCFSRI
                     ETNGGAFEQVNLLYGENPNDVVRQWVKPFKEAGIQTHMLERALNGIRMYPVPADVRQL
                     MYKVKKAQGVDIARSFCGLNDPRNLELSIKYAKEAGMISQAALSITHSELHTTDHYMA
                     VVDEAIKYGADEICLKDMAGIGRPTEIGRLISAIKSSYPNVVVQYHGHAGPGFSVASM
                     LEAAKAGVDYIDTAMEPLAWGTSHPDVITIQSMLKDAGFKVPEINMKAYMHARSLNQE
                     FMDEFLGLFINPKNHLMSSLLIGSGLPGGMMGSMMADLEGVHQGINQSLAAANKPQLS
                     LDDLLVALFEEVQYVWPKLGYPPLVTPFSQYVKNVALVNVINLLQDKPRWQLFDKNTW
                     DMILGKTGKLPGPLDSEIIAIAEAQNLKFYDGIPQEAYPDALDQYREEMKKNGWEVGE
                     NEEELFELAMHDRQYRDYKSGIAKKRFEEELENAVANSKSASAGELIAIKAPEKGKIL
                     FGVSFNNGKRNPDKGDIFKNDERICYLETITNYETISCPANLKINKIFFKHGDVVQKD
                     ETILLGEPL"
     gene            54647..56308
                     /locus_tag="PLK75_01245"
     CDS             54647..56308
                     /locus_tag="PLK75_01245"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOY37901.1"
                     /translation="MAKKNISSVHERISKEIASHSNLIVNASRKTFIKDSVDNKECVV
                     SKYGTLATWTPIDSTGRSPKDTYAVKRASSEMHIDWTSPNNLPIEEDTFNMLLDDALT
                     FAKESDRLYVTDRVIGADSKYALPVKLITDKALPGLFADNMFRPVPKDISKSCFANKP
                     YFLLALPYRKLDHTKYTGRLRKLADGTTSDLVVAMDFDRGIGIVIGTAYLGSIKKLMF
                     TVMNYYLPFEGVLPLHCSANEGKDGKSALILGLSGTGKTTLSADPSRALLGDDEHGWS
                     EDGIANFENGCYAKMININPKKEPDIYKAVLHQADYLKHGAIIENAMMYPNGEFDFDD
                     ERLTPNSRASYPLTSIKNIKKSSTAGHPSTILFLTADAYGVIPPVSKLDKSQAMLWFL
                     MGYTSKVAGTERGVVEPSATFSRFFGQPFMPGLPSVYTKMLGEKMEKFNTSVYLINTG
                     WTAGKFGVGHRIDLVLTRKMVNAALNGDLEKVDYIYDKLFHLSIPVSCPGVPSEILNP
                     INTWKDPEDFRKTANELAQKFSKTFDDSFGKGHIDPLIRKNCPGK"
     gene            56428..58470
                     /gene="ppk1"
                     /locus_tag="PLK75_01250"
     CDS             56428..58470
                     /gene="ppk1"
                     /locus_tag="PLK75_01250"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOY37902.1"
                     /translation="MKTTFTNREISWLAFNNRVLQEADSEEVPLYERIRFLGIFSNNL
                     DEFYRVRVATLTRLYKLKEKDFPEKAIIYKDLLDEINDINAHNQNEVSRILQNIQDRL
                     EEKNVFLISEKELSPEQGSFIRQFFKSYVRPNLFPVILKNMQHPSFLKDSSIYLAIIL
                     KRHGFAEKTTYAVVEIPVKEVDRFIVLPQAGNKHYIIRLDDVIRYCLNDIFGVFGFDQ
                     FQAFTFKLTRDAELDIDEDISKSFVELISNSLKARETGNPVRFIYDKEMPSSLLNALM
                     KALKIRKRDTVMQGGRYHNTKDFMNFPHVSEKVLSYEPIVPLPNKFIQPGKKITSSIR
                     ERDILLHYPYQPYQYIIDLLREASIDPKVRAIKMTLYRLANPSKVVNALVNASANGKQ
                     VTVFMEFQARFDESNNIYYSSKLQEAGVRIIKTIPGFKVHCKMILIKRIEGDKEKLYA
                     NISTGNFNEATSRIYSDNTLLTCDPRITSDVEKVFELFERSYKPMRFQHLVISPFSTR
                     NFFLSLINNEIKNAKAGKPAWAIIKLNSLADEQLSRKLLQAAEAGVALSLIIRGICIL
                     NPEGHKNIEIISIVDKFLEHSRVLVFCNGGKNKYFISSADWMIRNLDNRIEVTTPIYN
                     PDIQQELLDMLKIQLSDNTKARIIDNTLRSKMKINDQPKVRSQIDMYNYLKKKHLS"
     gene            58467..59351
                     /locus_tag="PLK75_01255"
     CDS             58467..59351
                     /locus_tag="PLK75_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37903.1"
                     /translation="MIFATIDIGSNAGRLLIAHVYEKFRVLFSSKIALVRVPLRLGMD
                     VFEKGYISEDKIQDLIKALQAYKLIMEIYKPVAFDACTTSAMREAANREDVLQRVIDA
                     TGINLRVIDGIEEAQLISEANNIYINRIYKDTLYIDVGGGSTECSLFSGERFITSKSF
                     NIGTIRLMFDKVKSSEWDDLKQWLESMPVRESQLNCICTGGNISAITKLFGNTDHTID
                     YPQLIHACEELEKYSYEERMVKYSFRPDRADVIVPASKIFNNIMKWGCINRLIAPQLG
                     LADGLAIELYKNHLKSGL"
     gene            complement(59334..60215)
                     /locus_tag="PLK75_01260"
     CDS             complement(59334..60215)
                     /locus_tag="PLK75_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOY37904.1"
                     /translation="MNEKDVKSLFLLLEDPDPKVYEAIRSSIFEHLDEVKPYLEQSWV
                     LSSDILYQERIQLIHDEIEAKKYVFQLQKWAGAENPQLFEALLIVERALFPEYDTVEL
                     PDAFRKIRHNIWMELTNSSTAIEKIKTMNQVLYNVFCLSKPVENVVKREHLCLSHVIS
                     QKTGDPLMQCLLYASLAQNLGMPVFVIALPDLFVLAYKDAFIAELTFATEKHYDVLFY
                     IYPWEQGALFGRSVIDKYLEEKQLSPAPEFYTPADNVKTVHTYLAVLRNSFSLGKNTE
                     KQQQIIRQMMNVLEPRL"
     gene            complement(60212..>61195)
                     /locus_tag="PLK75_01265"
     CDS             complement(60212..>61195)
                     /locus_tag="PLK75_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOY37905.1"
                     /translation="LVRVPVGATALDFAYEIHTDIGNTAIAAKINHQLRPLSQELRSG
                     DQVEIITSQQQLPKREWLSFVNTSKAKGKIKNALKETRRFNIEKGQQLFIQKVRELEI
                     QLDHELFKKLRDNYDVPNKEEFYRRIGQGIILLDDIEKHLSNKKPSIFKKYFRLQLSK
                     PKNQTGDVIKKGKVIDVDPELFEENFTIAQCCSPIPGDDVVGYLNPNNSVIVHRKNCA
                     TAINLMATQGDKIVSVHWKSQKLMSFAVRVQIRGIDETGMVFKITQVISSEANVNMKE
                     LHFVTKDGVFTGNIKMFVHDTSDLEQLIQKILRIKGVNQVVRIEENELNLQ"
BASE COUNT        17670 a        13602 c        12503 g        17320 t
ORIGIN      
        1 tgactatttc gtggtcgggg ctcccaaatg atacggtagt tctcgcaggc atgcttcgaa
       61 tgtgttgtcc ggctatccca acatacactt cgctaagctt aattcctgaa atttcacttg
      121 ctttttctac ggcaagcctg atagtttcga tagcctcctc aatatttaac accgaacccc
      181 gttttattcc ttttgaagga accgtcccaa atccaaggat ttcgtactta ttttcagagt
      241 ttctttttcc ggtaatggca acaatttttg ttgttccgat gtcgattgct gctataattg
      301 aattatccat aaacatagct ttattttgtg caaacaattt gatttgcgta tttgaggttt
      361 attgttttgt aggagttcca gtcggtgtgc ttaaatcctt cataatacat tgcttcaagt
      421 ttaaaaagct tgtactcata atcggttaaa tcgcccaaaa ctataatatg atttccaact
      481 ctgggaacca attcaacttc gtaatttgca ttgatattaa tttgctcgaa ttgggggctc
      541 cataactcgt tatgatgtat atacgatacg aaatgatata aatcgtataa agtaaaatga
      601 taggtttcaa tcacattgcg gttcttcaca ctgtcgttgt cgatgctgga gataaactga
      661 ggtgttattt cgccggtaat tactggtaca tgagcagtgt atttttccga taagggcatt
      721 atttttccct gatcgtcgat ataaaaacca ttattgagag aggtatattt agggataacg
      781 cgtactattg gaacacgctg aaccaccgaa acaatcagct taccctcata ggatgaatat
      841 acttgtacat ctcgcaccgc agaatggttt tttatggttt tttcgatcaa ctcaaagtta
      901 atatgactta atggaatact atcataaaac aacttattgt tagtcaacaa tttattaaca
      961 tcagcactgt cgataaattc gctctcacta ttgacagtta tacgaagcga actgcacagt
     1021 gctttggttc tcattttatc gacaaaactc ataaccacaa tcagatacgc aatcagtagg
     1081 ctccatttca atattttaat aatcttattt ttcattgagc aggatgtttt ttatgggttg
     1141 caccattttg tcgatatcac cggctcccat agtaataaaa acctctgttt tgatgcattt
     1201 ttcaaccaaa cccggtaatg tgcttttggt acaggttatt ttatcggcga tagtaacttt
     1261 ttcgagtatt atttctgatg acacaccttc aatgggcaat tcccttgcag ggtatatgtc
     1321 caacaatatc agcctatcaa gtttcgatag tgattctgca aaatcgttcg caaaatcgcg
     1381 tgtacgtgtg taaaggtggg gttgaaaaat gcctgtaatc ttccattcag gaaatagcaa
     1441 tctggccgct tcgattgtag cagcaatttc gcgtggatga tgcgcataat cgtcgataaa
     1501 aaccagcttg tctgtattaa taatatactc gaagcgtcgc ttaacacctt tgaagcatga
     1561 gagagcgttt cgtacatgtc cggcatcggc acctgcgcaa agtgcaattg ccgatgacgc
     1621 aatcgcattt tccacatgat gccgcccggc ataagggaac acaatttcgc tgattactcc
     1681 gaatggagtg tgcaaatcga aatggcagcg actgtgagta atcgttatat tgtttgcgta
     1741 aaaatcggag ttcacgtcgc tgaaagaata agtcagcaat ttttgcccac ccgtaagaac
     1801 cactttatta cggatatcat tcttcagaat aaccgtaccg ttgtgtttag tctgattggc
     1861 aaactggtta tacgcgtttt ccatattctg agcattgcca taaatgtcga ggtgatcagc
     1921 atcgatatac gatatcaggg caatgtcggg gaatagcttc aaaaatgacc tatcgtattc
     1981 atcagcctca acaacaacga gttctgaatt tttatgaagc accagattcg attgaaagtt
     2041 accggaaata ccccccagaa aagcaccagc tttcagtttt ccctgattaa gtatccaggc
     2101 agccagggta gaaattgaag tttttccatg tgttcctgca actgcaatgc aagttttgag
     2161 agcagctatt tcggcaagta tttctgagcg cttcagtact tcgaaaccat tacttctgaa
     2221 atactcaagt tgtttgtgcg aagggggcac tgcgggtgta aataccaccc tgcagcagaa
     2281 cttatcatgg cactgtgcag gtatcgattc gaggtcctcg tcaaaacaca tcgatatacc
     2341 atcatccgcg agtgcatcgg taatctctga ccgggagcgg tcgtagccgg ccacaaaata
     2401 accctgctta gagaaataat gtgccagggc actcatacca atgcctccga taccaataaa
     2461 ataatatgca atattacttt gatccttcat ctgcaatttc aattactttt ttagcaatag
     2521 tttcggctga gttaagaatt gccatttttt gtatgttctc cgacaatttg ttttgaagtt
     2581 ctttgttccc aacaagcgat aaaatcgcag gtgctaattc ataggatgcc tcaatatctt
     2641 ttaccatcag cgccgcatct ttgtttacca aagccattgc gttttttgtc tggtgatctt
     2701 ctgaaacatt cggtgatggc acaaaaatcg caggttttcc gataacgcac aactcggaaa
     2761 tagtacttgc acctgctctc gacaccacaa tatcggccac agtaaaagca agatccattc
     2821 tgtagataaa gtcaaatgcc ttgatataat tactttttcc tgttcggata acttcttcag
     2881 ctttaccgat atacgatttc cctgtttgcc ataatacctg aattttttga ttttcaaata
     2941 cagatatatt attcccaata gcttcgttga tagtatatgc accaagactt ccgcccaacg
     3001 aaagtacgac aggtaattcc gggtccagcc caaagaattc aacagcttca cgctttttat
     3061 catcgagttc aattaagtcc tgccgaacag gatttccggt taaaattaat ttttcagcag
     3121 gaaaatattt atcaagacct tcataagcaa cacaaatgca acgcactttc ttagcgagca
     3181 tccggtttgt aattccagga taagaattct gctcctgaat gaggcaggga atacctcgcc
     3241 gtgaagccac cctgagaaga gggccactgg catatcctcc cacacctacc actgcattgg
     3301 gtttaaattc atcaacaatt ttccgtgctt gctgcgtgct tttcaatagt cctgtaaaaa
     3361 acttgagcat tttcaacgaa agcttgcgtg ggaaacccat aacaggcaag ccaacaatcg
     3421 ggtatccagc tttggggacc ttttccattt ccatcctgcc caatgcacca acaaataata
     3481 tttcaatatc actttcgata actttcagcg cattggcaat tgaaatagcc gggaaaatat
     3541 gtccaccggt tcctcctccg ctaattatta ctttttttgg gctcatttct gtcttggtaa
     3601 aatgctcatt taatattcag ttcttttata acacccgctg ccacagtctc ctcccgtgtt
     3661 tctgtgtctt cttccggcac agaactatct tctgtcacgg ccggggggtc ggggctctcg
     3721 ttgatttctt tcttttttgc ttcttccagt tcgtttactt ctctgctcac actaagtatt
     3781 acccccattg caaggcttaa gaataatatt gacgttcccc ccatacttac gaatggtaat
     3841 gtctgaccgg ttacagggaa aatattgaca gccaccgcca tatgcgacaa tgcctgaaag
     3901 acaagcagta aacccaaacc cgcgacaaga aatgctccga actttctgtc aaccttacgt
     3961 attataatta caaccctgta gaatagaaaa atatagaggg caataagcac caaacctcct
     4021 aaaaagaacc catattcttc gatgatgatt gcaaaaataa aatcggagta cgggtgtggt
     4081 aatacatttc gttgtactga atttccggga ccttttccca gaaatcctcc cgatgcaacg
     4141 gcaatttttg actggtttgc ctgaaaattg ccctcacttt cggggttcat aaagtcttcg
     4201 atacgttttt gcgctgttcc caggcggctt tcaataggac tcacctgaat tatcagtaca
     4261 aaaagaccca atccggccaa agcaataccc agcaccgaca gaatgtattt gaattttacc
     4321 tgcccaataa agagtaatag catcgaaata ccaaagagta agagggttgt acttattcct
     4381 gccggaaaaa ttaaaccgca tataattaaa attggcaaca tcaggtacct tagcatacct
     4441 ttaaaggttc caatatcatc ttcgtgtaca gccagcattc gtgccagata cattacaagc
     4501 gcaaccttgg caaaatcgct tgtttgaaag gttattcctc cgggcagaac tatccacctg
     4561 ctcgcctgat ttaggcttat tccccaaagc aaggttacaa gaagcaaaaa tacagcgagg
     4621 tagtatgcaa attgcgaaag cttgctaaaa tatcgatagg gtatccgatg tacgaaaaat
     4681 attatcagga aaccgacaaa taacaatgca ccctgccgca ataagtagtg agtggcattg
     4741 cccccgaaat acttatatgc aagggttccg gaggcactgt aaacagccag aagggaatag
     4801 aacgacaata taatgagtat cgtccagata accctgtcgc ctttaatatt tctttttata
     4861 aactctaaca ttacaagctc ctgacaattt ttttaaactg ataacctctg tcttcgtaat
     4921 tgctgaacaa gtcgaaactg gcacaagccg gcgacagtag aacactatcc ccgttggaag
     4981 ccagatgata tgctgcattc actgcttctt ccatcgactt ggtatcaacc atttgtggca
     5041 catattcctt aaaagctgca tgaatttttg aattatccac acccaggcaa acaatggcct
     5101 taactttttg cttcacaaga tcaaccaagc ctgaatagtc gttgcccttg tcaacacctc
     5161 ctacaatcca tactaccggt gatttcatgc actccagtgc ataccaggca gagtttacgt
     5221 tagttgcctt tgaatcgttg ataaactcaa caccatgcac tttgattacc ggttcaagcc
     5281 ggtgttcaac atttttaaaa tccataaggc tttcgcgaat aatgtctttt cttatatcca
     5341 gtacatgggc agcaatggat gcagccattg aattgtaaac attgtgtttg ccttgaatag
     5401 ctaaatcgtg tattgtcata gtcattgcgg tttttgaatt atccattagg ttgatttgtt
     5461 cgttattggt atatgcgccg tttgttatcg atgtgtcgtt cagcgaaaag gggtataacc
     5521 gtgccggagt gttgtatttt tcaagttcat taataataac ctgatcgtcg gaacaataaa
     5581 tgaaaacatc gttttccgtc tgattttgca taatccggaa cttggaatcg acatagttct
     5641 gaaatttata ttcgtaccgg tcgaggtgat cgggtgtaat attgagcaga attgccagtt
     5701 cggctttaaa atcaaacata ccgtcaagtt gaaaactact caactccaat acataatagt
     5761 cgtagttaca cgtagccacc tgaaatgcaa aactttgtcc cacatttccg gcaagaccca
     5821 cattaaaacc tgcctttttc agaatgtggt aagtgagcat ggtggttgtg gttttaccgt
     5881 tgctgcccgt aatgcagatt tttatggcac tggtgtaacg cgatgcaaac tctatttcgg
     5941 aaattaccgg gatatttctg gcatgcaatt ccttaattat cggtgctttg tcgggaatcc
     6001 ctgggctctt gataacctcg gaagccgaat agatgagggt ttcgctgtgc tttccctctt
     6061 cgaagccaat gttataatcc tgcagcatga ttttgtattt gggtgcaatc atactcatat
     6121 cggaaacaaa aacatcgtat cctttggcca atgccaacac agctgagcca accccactct
     6181 cacctgcacc cagtattaca atcttttttt tcaatgttag cgtagtttaa gtgtgattat
     6241 ggttattaca gccagcaaca gccccacaat ccagaacctt gcaacgatct ttgcttcggg
     6301 ataccccttt ttctggtaat ggtgatgcag cggcgacatg aggaaaattc ttcttccttc
     6361 gccatatttt cgttttgtgt atttaaaata ggcaacctgc attatcaccg aaagactttc
     6421 cataatgaat acaccgcaaa gtatcgggag aagcaactct ttcttaataa taattgcaaa
     6481 cacggctata attccaccga tggccagact accagtgtcg cccatgaaca cttgtgccgg
     6541 gaatgaattg taccacagga agccaatggt tgcaccaata aatgcagcca tgtaaacaac
     6601 caactcccct gtgttgggga tatacataat attcagataa tcggcagcaa tatggttccc
     6661 cgaaacatag gccaacactc cgagagtggc accgataacg gcagctgagc ctgcagccaa
     6721 gccatcaagt ccatcggtaa ggtttgctcc attggaaagc agggttatta taagcaccgt
     6781 gataaccaca aaaatgcccc atgcaaattt gtatttcaca ttttcgttta aaaaatttac
     6841 gaaccatacg taattgaatt cattatcctt cacaaaaggg atagaagttc tggtactttt
     6901 aatatcatga tacagcacat cgccttcttc gcaattcgat atagtgatat catcgccaat
     6961 aatttgctgg tcggcagtgg caggggtctg cgatttaacc cgtataacta catcatcgct
     7021 gaaatataac gtcaggccga taaccaaacc aataattacc tgacccataa ttttaaactt
     7081 acccgggagt cccttcttat tctttttgaa aacctttata taatcatcaa taaagccaat
     7141 tcctccaagc catattgtag ttgccagcat caaaatcaca taggtattgt cgagctttgc
     7201 ccaaagcaga accggaagta taattgccag aaggatgatg actccaccca tggtaggggt
     7261 tccctttttc tgcatttgac cttccagacc caggtcgcgg atttcctcac ctatttgcaa
     7321 acgctgcaat ttgcgtatga ttttcttgcc aaacaacatc gaaatgacca gcgaagtaat
     7381 taccgtcatc gctgccctaa atgagatata ctctattacg ccggcacccg gaacgttgag
     7441 ctgatcgaga taatggaaca aatagtacaa cattatttta gcgttttaaa tatttcagtt
     7501 acaatttctc tgtcatcaaa agggtgtttt acacccatta tttcctgata attctcatga
     7561 cctttaccgg caacaagaat gacatcccct tttttggcga ggatacatgc agtgcgaatg
     7621 gcttcttttc tatccacaat tttcagcgta ttggcctttg ccgactcgct taaaccttgt
     7681 tccatatcgc tgataatttg ttcgggaact tcgtttctag gattatcgga tgtaagtatc
     7741 agcctgtcgc ttaaaagaga agccactttg gccatttcgg gccgtttcgt tttatcgcgg
     7801 ttaccacctg caccaacaac ggttataatg ctttcatctt tgcttcgtat ctgttgaatg
     7861 gttgtaagca cgttcttaag agcatcagga gtatgggcat aatcgacaat ggcaataata
     7921 ccggaagagg acttaattgt ctgaaaacga ccatcaacag cgtggagtgt gctaagcaca
     7981 atcaggattt cttcgcgctg aaagccaagt tttattgctg ctccatacac tgcaagcagg
     8041 ttgcttgcgt tgaaacgtcc tacaaaatgt gtccatattt catgcccatc gatagaaagt
     8101 aacatcccgt cgaaatgact ttcgataatt tttgccttaa agtcggctac cctgtctaat
     8161 gaatacgacg acctggcagc agaagtattc tgcagcatta cttctccgtt tttatcatca
     8221 ttgttggtaa tagcaaatga tgacgagggt agcatgtcga aaaaagcttt tttagccttg
     8281 atgtattgat cgaaggtttt atggtagtcg agatgatcgt gggtaatatt tgtaaaaatt
     8341 ccaccggtaa acttcagtgc agcaattctg ttctgtacga ccgaatgtga gctcacttcc
     8401 ataaaacagt attcgcaacc ctgtcgaacc atttccctaa gcagtttatt taactgtaaa
     8461 gcatcaggtg ttgtatgcgt agccagaact tcgtcagcgc aaatcatatt tcttaccgtg
     8521 ctgagcaatc cggccttgta acccaatttc agggtaagct tatataacgt agtggcaatg
     8581 gtggtttttc cgtttgttcc cgtaacgccg accaatttca gtttctccga agggaaatcg
     8641 taatagaatg ctgctatttc ggcaagtgca agtgatgcat cgtttaccag tatccaacat
     8701 acattattat ttaaatcttc tggcaacttt tcgcaaacga ttgcagctgc tcccttagca
     8761 caaacatcct gaataaaact atgcccatcg gaaacagttc ccttaacagc aacaaacaag
     8821 tcgtcctgca ttacaatgcg tgaatcgaaa acaacatttg caaagctttc aacaccattg
     8881 gcaacaattt gttttaccgc aacatattta cataggtcct ctattcgttt catcccagaa
     8941 tgattttaat ttgctgtcct ttaataaagc tctgattggg ttgcggttcc tgttgaatca
     9001 ccttacctct gccttcaaca acaaccttaa gtccgaattc ctcaagcacc atcattgcat
     9061 cgcgtaagcc cattccacgt acatcaggta ctagattctt attggtatag ttacgcggat
     9121 aatacttaac tgcccgattc tcgacaaatg gctttgccca ttctcctttc gttacaaccg
     9181 actgcttctt gtcgaaaccc aagagaaagt aaaccttctg tatctctgac ttatacccgc
     9241 ttttggccat aggtataatg gtgtcgtatt tcatgtcgtt cttcactgaa aaaatagtat
     9301 ccattgcatg aattctatca gcaatttcct taaatacagg gcccgcaaca gaatttgcat
     9361 aatagcccgt tgaaatatca ggttgattaa ttaccacaat gcatgagtac ttcgggtttt
     9421 cggcagggaa ataccctaca aatgaagcct ggtactgaag aggtcctccc ggcttaccat
     9481 aatcggtctg ggctgttcct gttttcccgg caatgctgta attatcgtta atcaggtttt
     9541 tggctgttcc ccgtaaaaca acaccagcaa gcatatcctg cactgcttgc aatgttttat
     9601 cgctgcaaat tttttctacc agaacttcgg tttgaaaatt tttaacaata tgaccgcgat
     9661 atgtcaaaca ctctgcaaaa cgtggtttca ccatttttcc gtcatttgcc actgcattat
     9721 aaaatgtaag catctgcagg ggtgtcatca gcagttcgta cccgatcgac atctgcgtta
     9781 acgaaatctt tgaccatccc tgttccggcc ggcgaatagt gggtatagcc tcaccgctga
     9841 tttccactcc caggggctgg ttcaatctca tttcgtatag tttatcaaca aaatcgcttt
     9901 tgcggtggta aaaattttcg taaatcattt tcgagatacc cacgttcgat gatagttcaa
     9961 aggcttcctg aatggtaatt ttaccatatc ctccttcatg agaatctttc atctctttgc
    10021 catggaagtt atgtgtcccg tcttcagtat caatagtgtc ggtggggctt acgcatccat
    10081 cttcgagcgc aacaatcata gaagcaagtt taaatgttga tcccgggtca cgggcttcgc
    10141 caattgcata attgaacgat tcgtagtaat tgccattttc atcgcgggta aggtttgcca
    10201 tagcacggat tttacctgtt gaaacttcca ttactattgc acaaccgtgg tgggcattat
    10261 gataacgtaa cttacgttcc agtgcggttt cggtaacatc ctgaatatta atatcaatgg
    10321 ttgtaataag gtcgtagcca tctttaggag ccacctcatt ggcatcgcga ataggcatcc
    10381 attggtttcc ggcaattttc ctttttaccg ttttaccttc tacacctctc aagtcattct
    10441 catatgccat ttcgagtcca accccctttc tcttgtcgga atcaacttta ccaattgtgc
    10501 gctttgccaa catgccaaat ggcagctctc tttcagtata ttcatttata atgaggccgc
    10561 cttcaatctt accgtatcga agtattggaa aggtctttat agcttttagc tgatcgtaag
    10621 ttaccttacc gttgatgcgg taatatcggt tgccattatc acggctgtgg gtaatttcgt
    10681 taaagtattg ttcccttgtc ttaacaggta gcaagtttgc caggcatata caaaggctgt
    10741 cgatttcttt gtcgaacact tccttttcga ttccattgct attgggatca attccaactt
    10801 cgtagtaggg cactgaggtt gcgaggactc ttccgtcgca tgcgcatata tcaccgcgat
    10861 tcggtggaat ggaaactacc ctggttacac ttttcgatgc gttagtaatt accgtttcat
    10921 ctatagtaaa atatagccgg aatattttta caaaaataaa cagcccgaaa aacaggatga
    10981 tgaaataaat cactttaatc cgccataata tttcagttct cggggtcact tattcttctt
    11041 tttttcggat ttataaaaaa ttttcattgg tggtatcgac gattcgttta actctgaacc
    11101 gttctcctta agcattctca ttacttcacc gcgcctgctt ttccgcatca gaaccgactg
    11161 ggtggcaatt ttttcagagc gtagttcaac gatttccttt ttcagtttct cactttcgat
    11221 aaaaaccctt tcggcatgat agcggtttgc aatgtaaata agagcaagca ctgcaagcat
    11281 taccagaaaa ggaaactgcc tacgcacaaa atccgtagcc aggattgagc cgctcataag
    11341 tccctgtaag gttttacgca cctgaccttt cttcttttta ttttcagcag tctttgccat
    11401 ggttattttc tagttgctat gcggagcttt gcagcccggg acttactgtt catgttaatc
    11461 tcttcttctg atgcggctat tggttttttg gtaacaggct cccatggtcc ttctattttt
    11521 ccgtaaagat catgctgctc aactccttca atattaccag agcggattac atttttcacc
    11581 aatctgtcct ccagtgaatg ataagaaatc accaccagtc tgccttcttt ggcaaccacc
    11641 ccggtgcagg tattcaaaaa atcagcaagc gcattcactt catcgttaac ctcaatgcgt
    11701 aaagcctgaa acacctgagc caataactta ttcctccgta caggcggtat atacttttcg
    11761 gccaacgaaa caacaactga agtacttgac atatccatgg catctctgtt tgcaacaata
    11821 tctctggcca gttttgcagc aaaatctatt tctccgtaaa tgcgaaaaat ggttgcgagt
    11881 tctttctcgc catagttatt caaaacataa gcagcatctt tcactgcctt accattcatt
    11941 ctcatgtcga tttttgattc agaccgaaac gaaaaaccac ggcactcttc atcgaaatga
    12001 tgtgatgaaa caccaaggtc cgcaatgata ccgtccactt tatctatccc gaaataatcg
    12061 agataattct gcaaatagcg aaaattacca tgaataaaaa ccagcctttt atcgtcaggc
    12121 agattttgtg ctgcatcctc atcctgatcg atggcataca gtattccatt ttcacctatt
    12181 tcctctaata ttcgtcggct gtgcccaccc ccaccgaaag ttgcatcgac ataaatccct
    12241 tcaggtctga ttttcagtcc ggcaatgctt tcattcaaca tgacaggctg gtgatacatt
    12301 tgttctaatc ctcattttga taaaaaaagt ctccacccag aatgcgctcg gcaagattcg
    12361 caaaatcaga ttctccggtt ttcttggaat aatacatctt actgctccaa atttcaatct
    12421 taccatcctg acccgcaaaa acaagctctg tttgattttc acccaaacac tcaagcaagc
    12481 gttttggaat taataggcgg ttgctggaat ctacaaggat ctcagcagtt tctctatgaa
    12541 attcgcgtaa gaataaagca tgctcccgat taaacggatt cagtttttta cgaatcgccc
    12601 gattttgctt ttcccattct aaaattggat acaacaccag acattcctca tacacatcgc
    12661 gtttcagaac aaaacgatcc tgctgatcgg caggcaattg ccttttaagg gctgcaggaa
    12721 acaataccct tcccttatta tcaagcttgc aactgtattc tccaacaaac ataattccgg
    12781 tattaatttg ttgctaagat atataaatac tcccacttac atattctttt ctcccacttt
    12841 ttcccacatt gttgaaaact tttacactca ataattctat tctattgatt atctgacaat
    12901 tatgatgttt gattttttta tatttttgac aaaaattaac aaatggggat atgtcggaaa
    12961 aagatgtgct aaaaattgac gttgagaaag ttatcgacaa caaaaatccg aagttgcgga
    13021 aaaagcttcc taaatttctg ataaattacc ttaaaaagat tgtccatcag gaggaattga
    13081 atgagttttt caaagaatca cacggaaaaa cgggtcttga ttttgtggat ataatattat
    13141 cgtactacaa cataggcatt gatgctgagg gagtagaaaa tatacctgat aaaggaagat
    13201 ttgtctttgc tgcgaaccat ccgataggcg gtatcgaaag catagccttt atcaaagtgg
    13261 tttcagaaaa atacaaagaa ttaaaatttc cggtgaatga tattttgatg gaactaaaac
    13321 ccatggcaag catatttata cccatcaata aacacggagg ccaaagcaaa tttgcaataa
    13381 aagagcttga agattatttc gcaggaaacg gacagatttt atattttccg gcaggaatgt
    13441 gttcgcggcg tatcaagggg aggattcagg accctgaatg gaaaaagaca tttattacaa
    13501 aagccattca gcacaagagg gatataatac cagtgtatat tgccggcaga aacagtaatt
    13561 ttttctacaa tctttccaat ttcagaacat ttgcaaggat taaaacgaat ttagaaatgt
    13621 tatacttggt ggatgaaaca ttccgacagt ttaacagcac cattcgcttg aaattcggaa
    13681 aaccaatcag ttatacgcat tttaatgatt cacaaaccca ccaacaatgg gcagaaaatc
    13741 ttaaagaaat agtctaccaa ttgggtggaa tacagctgaa acactgattt ttttatcacc
    13801 gttgctattt ttttagataa aatacccaat tagtattaat tttgcggtga aaatcattac
    13861 cgaaaatgga atccgttatc ccgcccgttg atagaaaatt gctacttaaa gaacttgccg
    13921 acggaaagct actgcgggag tcgaatatgg gcaaaactga aatttacgtt ttctcgcacg
    13981 aagactctcc caacctgatg aaagagcttg gaagggttag ggaaattact ttccgtgatg
    14041 cgggaggcgg gacggggtta tcgtgcgaca tagatactta cgatatttcg gaaacaccat
    14101 atctacagct gattgtttgg gacccggagg agcttgaaat tatcggtgga tatcgtttta
    14161 ttcatggcgc aagcgttctt gataatccta tcgaaaaact ggctacttca ccattgtttt
    14221 cgttttcaga tctctttctg aaagaatatt taccctatac aatcgaactt gggagatcgt
    14281 ttgtgcaacc caaataccaa cagggaaatc aggcgcggaa agggcttttt gcgctcgaca
    14341 atctgtggga tggcattggt gcgcttacca ttgaataccc cgatataaag tatttcttcg
    14401 gcaaggtaac catgtataaa agttatgatg tggaagcccg aaaccacctg ctggctttta
    14461 tgaacctgta ttttcccgac aagcagcacc tggtaagtcc catagcaccc attgaagtag
    14521 attttaaaaa atatcagaaa gcattcaggg gaaatagcta ccgtaacgat tataaaacac
    14581 ttagcaacat tatacggaaa tcgggtgaaa gaattcctcc ccttataaac acctacatga
    14641 acatttcgtc atcgatgaaa acctttggca ccgttgacaa tccacatttc ggagcggttg
    14701 aagaaaccgg catcctcgtt aacattagcg atatatattc atcgaaaaaa gatcgtcaca
    14761 tactaactta caagcgaatt tcgaaacttt cttccattat ttcgagtaag ctgaaaatga
    14821 aagttaaaca aaaaaggaaa ttatagcatt ataatcaggt tgttgcgaat gtgtttttta
    14881 aacctgtaaa ttcttggata aaaaaatatt ccgtaaatca tgtaaggaat aataattcct
    14941 ccgataaaaa taattagatt aaaagctcta tggcttgccc ctgatgaaaa atcagcaaga
    15001 atcacgattc tgttcttttt aaggaacacc cttgctcctt cgtatttgga acccgatact
    15061 ctaacactgt ttctgccgaa acgtgtcaat ccctttacac cttctatact tctgagcaat
    15121 tccattaaat ggctcatatc ggttgttttc tccgaaataa ctatcttcat aatcgcagca
    15181 agtttttcag tttaaaaata aaacataata atcattcaaa caattagttt tataattttg
    15241 aatgaattta taaaaatgaa aaaagtcgca ttattcattc ttgtcattat tggcttctct
    15301 gttttaaatg cacagaatct ggtccccaat gccagtttcg aaaatgcatg gacatgccct
    15361 tcgtattttt atactgaggc tgtaggcgat atcattccaa tgtggcacaa ccccagcaga
    15421 ggcactcccg actattttaa ccggtgttcc gacagtattg tttccgttcc gctcaacttt
    15481 gcaggcacac tcgaagcaca tgatggaaac ggatacatgg ggcttattct ccgtgaaact
    15541 ttcgaacctg agctgcggcg tgacggtgta tctcgggaat atattcaggt aaaaattgct
    15601 tcggccctca aggcagggca tctatattgc ttcacaatgc aatacgcact ctcatcaagg
    15661 tcgccatttg ctgtcgatgc ccttggcgtt gccattacaa agacaaagat atcatcgcga
    15721 gatgcaagac aaataattca gatgcctcag ataactaata ttccgggaca tttcatgcag
    15781 aataaaactg gatgggctga attatgtggc atttacagag cccgtggaaa cgagcaatat
    15841 cttactatcg gcaacttcct gaatgatgat aaaactagtt ttataccaat gaatgatgct
    15901 gatgctgact cgaattttgt ttatgcatat tactacattg ataatgtgaa acttatcgaa
    15961 attgaggatg aatttcagtg tgggtgtcag gatgattttt cggttggcag cgattatctt
    16021 tccgaaaaat acgaccgaaa aacagggttt aacacacgca aaccggataa ctctgataac
    16081 aacgggaacc tcgccgataa tcggaatggc ggaaacacaa ccctaattaa cgggaatgca
    16141 gtagataacg gaaataatcc aagcggtaat aacgggaaca atgctgaaaa tgccggagat
    16201 agcgataaga attatagtaa tattggcaat aattctgacg aaaacaaagg aaccgatgta
    16261 agtggcaact acactgcagg caattcgaat gaaaacctgt cggataacac caaagatctt
    16321 gacaatttcg acattgcttc agcaggtatc ggcgatgctt ttcgtatgag ccggatttat
    16381 tttgaattca actctgctga attgctcgac ccctcattcg accaaatgaa taagttgttg
    16441 gttcttctaa aagaaaatcc acgcctgcgc attgagatac gcggtcatac cgataacgta
    16501 ggtacaaagc aatacaaccg aaaactttcc attgcaaggg cagcagcggt ttacaactat
    16561 ctgattgatg ccggtatcga aaaaacacgt atgaaatacc gtggtttcgg caacgatgtt
    16621 cctgtggcaa caaacgacac ggaggaaggc cgacagctca accggagggt agagatttta
    16681 attgtggaac tgtagtgttt taaccatgaa attcagcaga tcaatctcct tcaaaatttc
    16741 gctgctcgtg gtgtgtctgg tcgtgctctc tatatttatg attgctttat tctcctacat
    16801 ccaaaccagt aaagccatta tcgatagaac tttcggacaa ctcaactcgg tgaaatggga
    16861 aaaaagccgc cgtatcgaga acttttttgg tgaacgcacc aatgaactta agctttttgc
    16921 ttcctcgaaa cctgttgctc aattgtgcga attgatggaa agcgaaaacc tgaaaacagg
    16981 cgaaaacctg aaaaccagct acgagaacga agcatacttt tcaaactatc tgttgctgca
    17041 tcccgatatt ttaaaattgt ttatcataaa taacaacaac cggttgattg aaacatcggc
    17101 acaacaacgg gtttttatta gtgcatcgcc atcagaccca ctgaaataca tttcggatac
    17161 catttttaac agtacattca gccaacctgt gtattacacc gacataattc aggattctgc
    17221 aaataatccc gtattgtttg tattgctgcc tctcgacgtg cccggcaata aattcataat
    17281 cggattcgta attaatgcgt cggctatttc ggatatcatg tacgaaaaca acccattcaa
    17341 cggcatggga aaatcgggtg aagcgtatct ggtgggcagc gatactctca tgaggagtca
    17401 gtcgaggttt gtccccaatt cagtactgaa actaaaggct aaaaccgatg cggttattaa
    17461 tgccctcggc ggcaacgaca acacgggaat aataaacgac taccgcggaa ttaaggtgct
    17521 cagctcgtat ggtatcattt cgataaacaa tattcgttgg gcaattatgg cagaaatcga
    17581 ctataaagaa gcgattattc ccattgatga ctaccgcaac aacatgatat ttttgggtat
    17641 gctgtcggca ttgcttttca cggcattcat agtctttgta acccatacca tcattaaacc
    17701 ggtggttaaa ctcaaaaatg cagccctcga aatcgccaaa ggtaactacg atactgtgat
    17761 cgaaaaccat agctccgacg aggtgggtca gctaacactt gcctttaacc gcatggcaca
    17821 gcaactggat gaacaacgca aaaatcttga aaaggaacgc atcagccgtt tatcggagat
    17881 gatagactgg caggaaaacg agaggcaacg cctgtctcgc gaattgcacg acagtctggg
    17941 tcaggtttta ctaagtatta aaatgcgtat tcagcgtgcc aaaggaaaga ccgacaacga
    18001 caacgccata atcgacgaaa ccaatatgct gcttgccgaa accgtgcagg aagtacgtaa
    18061 tatctcgaac gacctgatgc catcggtact gatatcctac ggattgcagg gttgtttgaa
    18121 aaatctctgt gagaatatcc tggcacgagc cgatatcgaa gccgatttta aaaccaatat
    18181 cgaaagccta ctcccttcga aaatacagac ctatctgtac cgaattatac aggaagccgt
    18241 caacaacatt atcaaacatt ctaaagccac ccgtgtgagt atcgtgctga tgaattacga
    18301 ttcaggcatt gaactcacgg taagcgacaa cggttgcggt ttcgggcaag acattctgtc
    18361 ccaaaatcgt ggacagggac tgcgtaacat taccgaaagg gtggaacttc tcggtggcag
    18421 ttgcaccatt gattcccata atggtcaagg aactcagata tttgtaaaca taccgaataa
    18481 accccatgaa taaacccatt agtatttttt tgctcgacga ccataagcta ttccgcgatg
    18541 gaatcagggc tgtgttgtcc gacgaaccta acatcagcat tacgggtgaa gccggaagtt
    18601 ctgtcgaact gttccgcttg ctcgaaaata cacatccgga tatcctgata gccgacatcg
    18661 aactgcccgg tatgtcgggc atcgaaattg ccgcaaacct gcaccagacc agacccgaaa
    18721 taaaagtact ggtaatttcg atgcaccaga acgaggagta tgtgatgaat gcatgccgcg
    18781 ctggtgccat gggctttcta cccaaagaaa cgggtaaaga agagctggtt accgccatca
    18841 accgcattgc cggaggtgac acctattata atgccgacat tacagggaaa atcatacgca
    18901 aaatatcgga tcacacccgc aataccgaag tcaatccgct aaccgaactt tcgaaacgtg
    18961 aaatcgaaat tctgaagttt tttgccgaag gctattcaaa cctcgaaatt gctgagaaac
    19021 tctttatcag catacgcacc gttgaatcac acaaaaacca tatcatgcaa aaactgatgg
    19081 taaaatcaca ggttgatctg gttaaaattg ccatacgcta caagttggtg aacctgtaat
    19141 tcttttacgt gaatcactga aaaatatttc agcgaagcac ggtctctgat tcagaattcc
    19201 tcatacttca aaacagtttg attaacacaa atttgtgttg gttaaaattt gtttcacgta
    19261 atctatattt tatgaaaaaa ctttttctaa gtctcgttgt cgttagcatg acgataatgt
    19321 ttaacatcac tgctgtcaac gctcaggaat caccttttca tgttggcgtt gatatctgga
    19381 gtcgctatgt ttggaggggt accgattttg gttcatctcc cagtgtgcaa ccctgcatcg
    19441 aatattcgac tcatggcttc acaatcggca gttggggcgc atataccacc agcctgaacg
    19501 cacccgcaca ggaagccgat ttgtatcttg cttacacctt tgccaacgac atgtttaaag
    19561 taatcgtaac cgattatttt ttcccgacag atggagggtt gaaccaatat ttcaattaca
    19621 aaaaaggaga aaccggtcac tatttcgaag ggggtattgc ttttaacgga accgagaaac
    19681 tgcctctgaa gtttttcgcc ggaaccatgt tctatggcaa cgacctcgac tatgcaggcg
    19741 acaatcgctt ctcaacctat cttgagctgt cgtacaaccc cacgattaag ggaactgatt
    19801 tctctgtgtt tcttggtgca aacctcactg cggcatccga tgatgacctg aattacatcg
    19861 acccagtaac actgcaaccg gctccggtta gcggttttta tggcgataag gccggaatat
    19921 gcagggtggg tatcagtgct gcaaaaaaaa ttcagataac cgacagtttc gaacttcccc
    19981 ttgcggtgat gctccaaagc aatcccatga agggcaactt gtttcttaca gccggattca
    20041 cattttagtt caccaattac ttaattaaaa ttacaaggag gatttatgat tttaaatgca
    20101 attttcgact caggaacaac aacgttcatg attctgtgca ccagcctggt aatgcttatg
    20161 actccgggac tggcattttt ctatggcggt atggccggta agcgaaatat tctgggcatt
    20221 atgatgcaaa cctttgtatc gctgggctta accaccgtga tgtggattgg attcggttat
    20281 tcgctttgtt tcagtggagg tcagggtggt gtaatcggta acttcgacat ggctttttta
    20341 agcaatgtac ccttcgatca ggcatttgac cccatcaatg ggaaaaatta tccgacctac
    20401 atttttgtag cctaccagat gatgtttgcg atcatcactc ctgcccttat taccggtgca
    20461 tttgtgaacc gtgtaacctt taaatcgtac ctgattttcc tcgtgctatg gcaggtgttt
    20521 gtttactatc cctttgtaca tatggtatgg ggcggtggtt tacttgccga atggggtgta
    20581 aaagacttcg caggaggtat agtagtgcat tgtacagcag gttttgcagc attggcttcg
    20641 gttttctatg tggggaaacg tcgcgacagg caatctcctc ccaactcaat tcctttggta
    20701 gccatcggaa ccggtttgct ttggtttggc tggtacggat tcaacgcagg gtctgaactc
    20761 acggttgacc aggttacaac gctggcattc ctcaacaccg atacagccgc ttcgtttgct
    20821 gctgttacgt ggcttgccat cgaatggata cacacaggga aaccaaaatt catcggctta
    20881 cttaccgggg ctgttgccgg tttggcaacc attacccctg ctgctggatt cgtaccgcta
    20941 tgggctgctg tactgattgg tatcagcgcg ggtgccgtat gctatctggc agtatcgctg
    21001 aaaaacaaac tgggctggga tgacgcgctc gacgtttggg gtgttcacgg tattggtggt
    21061 ttgcttggga ccgtacttct tggtgttttt gccatgaaat cattcggcgg tcaatcgggg
    21121 cttatcgaag gcgactatgc tttcttcggg aaagaactcg tatcgattgt aattgccgct
    21181 gcctatgcgt ttatatttac ttacctgatg cttacaataa ttaactttat taccaaagtg
    21241 cgtgtttccg aagaacagga agtagtaggc ctggatgaat cgttgcatgg agagaaagcc
    21301 tacgacgaag gtgccctgta gtaccatcgg tttttgtatt ttacaaaata gccccgtgac
    21361 tggggctatt tttttcaata atacatcgtt aacaaccttg tgaagaaacc ctgatgtaaa
    21421 ataaatttct cattatattt gtggtaatat acacctatgt tgggaaaaca ggtttcgcac
    21481 ggtcacacta ctgttgcaac gggattaata cttctgctgc ttgcatggtc atgcagtcaa
    21541 gtgaaacatg tacctgctga caagtatctg cttgcaaatg ttaaaatcaa atgcaacaat
    21601 tctgctattg atcccgacga cctggagtca actttaaaac agaaacccaa ccggcaaact
    21661 ttcggaatat ttaagttcca tctgttgatg tacaatattg caaaaacggg aaaagagcgc
    21721 aagtggaaaa acaaaattgc caatgttgtg ggagaacctc ctgtggttta cgacgaaacc
    21781 ttgcgtaaac ggactatgca aaacatcaat tactttctga attataaagg gtactatcat
    21841 gcaacagtga gtgatactgt gaaattcaaa aggaaaaaag ccagacttac aattaatata
    21901 aacactggaa agcctcacat tattaacaat atcagctacg acatacagga tactgaggta
    21961 aaaagaatcg tagaagcgga tgtaacttca tgtctgttgc ttaccggcgc ccccctcgat
    22021 ttcgatgtga tagaggccga acaggcacgt cttgttaaac tgatgaaaaa gaacggttat
    22081 tacgatttta ccatcaacaa catacatttc tatgccgaca ccaatagcgt tcctttgaat
    22141 gcggacatta ccattgcaat aaggcatagt tttacaggag aaaacgatac caccggaaaa
    22201 ttcgaaaaat acaggattgg cgaagtatat atttttaccg aatacgatca aaaaaaatat
    22261 cttctcgaac gggacaaata cctgcattcg ctgattctta gcgagtttga agggtatcat
    22321 ttctacagtc cgggaaacct taaaattcgg ccactggcaa taatacaaac aatgttcatt
    22381 aaaaaaggtg attattacga tatttcgaac atcgaaaaaa cacacagcca tttatccgga
    22441 ttaaaacagt tcaaattaat aaatattgat cttgtggcag acagcgctga ttctaaaacg
    22501 ctgaattgtt acatatacct tacatcactg aacaagcaat cttatagtgt ggaaatcgaa
    22561 ggaaccaact cttcagggaa cctcggagca gcggggatta ttacatacca aaaccgcaat
    22621 ctgtttaaag gtgctgaaaa tctttcggta aaagggtcgc ttgccctgca aacgcttact
    22681 caggtggaag gtatcgaaaa ccggttcctg aacacttttg aaacttccgg agaaatatcg
    22741 ctgaatgtac ctaaactcat acttccattt taccgcaacg atgagtttgt taaaaataaa
    22801 ggtcccaaaa cccaattttc cgtgtcgaca agttatcaga aacgccccga ctatacgcga
    22861 ttactttcaa acgcaactat aggttattac tggaaaggtg gtgaaaacaa ttatctgacg
    22921 cattttttca acccgattga attgtatcag gtacgtattt gggatttcaa tcctgaattt
    22981 atggctggaa tcgaaaactt attcattcgc tacagttacg aaaatcaatt catttctgta
    23041 atcagttacg atttactcta caccaaccaa aacctaaaca aattaaagaa cttctggtat
    23101 ttgtggtgga acgtcgaaac tgcaggaaat ctgatggaag ggattttcga gttgtcgggg
    23161 caaacgcctg tagaaggatc gtatcaattg ttcggaactg agtttgcaca atttgtaaaa
    23221 acagatatcg atttaaggtt ttatcaggta ttcagtaaga aacacagcct tgtttaccgt
    23281 gcctattttg gtattggcat accttacggg aacagtaaaa gtaatggcct acctttcatt
    23341 aaaaagtatt tcacgggtgg cgccaacgac atcagggcat ggcaggtcag aactcttgga
    23401 cccggatcat acaacagcac ttccctgatc aatcaaatgg cagatatgaa attgatgtta
    23461 aatgcagaat acaggttcga tattgcatca tttcttgaag gagccatttt tctggatgca
    23521 ggaaatattt gggctattga taaagacgac gacagagaag gtgccttatt tacacccgga
    23581 acattttata aagaaattgc actgggcaca ggcattggcg cccgcttcga cttttcattc
    23641 tttataattc ggttcgattt cggggtacca ctatacgacc ctaaatatcc tatcggggaa
    23701 aggtggctga atacgtttag cacttttgag ttcagcgacc tgacctttaa ctttggtatc
    23761 ggctatccat tttagtcgat tccttcttaa taatacttct atggcctgtc cttagtaaaa
    23821 taaagataat tttatttgcc atataaatca aaatgcgaat gattaaaaaa ctcagtgttt
    23881 tcatttttct ctgcattggt atcagcagct tcccacgcac catcacggac acatcagaga
    23941 ccgtcatcct taaagtactc tcatggaata ttcagatgct gcctaatggg tttgatattt
    24001 tttcggagtc tctgcgtaaa ttacaacatg tacgggttga ctggataatt gaacattgcc
    24061 ttgcgcaaga ctatgatatt attgttttcc aggaagtatt cgatgtgcag atgaagcata
    24121 aaatacgcaa acacctgaag tcggaatacc cttttcaggt aaatacgcgt acaaaattcg
    24181 gtcgattcac gagcaatggc attctgattg tcagtaaact acccatcaaa tatatcgacc
    24241 acgtgatata caacaggggt gttcatgcgg atgcatgggc atcgaaaggc tgtacgctgg
    24301 ttgaaggaga gaaggacggt cacaaatttc aacttgcagg cacacacttg caggccggtg
    24361 ggtcaaaaaa agccaaattt caccgcagaa gtcagtataa agatatcgcc cacctgattg
    24421 aaaaaaacaa aaaagaccgc attccggtgc tggttgccgg ggatatgaat accggcaatg
    24481 atgaaaccga agattatagg cacatgctgg aagtgatgaa agtattcgac tttcctgtta
    24541 acgacacctg tccgtttacc atcgacagca acaattcgtg gaatttcaat tccaggagca
    24601 ggcaacttga ctatatcctt gttcaggcac gcaacactgt gacccggata tacaatcagt
    24661 atattttacg tttaacgcgc gatttttcgg gaaaagcaac cgactattcc gaccattatg
    24721 gtatagttgc tgaaattgag ttgggtaact gatcaagaaa ttttggcttt cttcaatctt
    24781 cccagttcat acaacgcgaa actgctttct tccatttacg gtacaacgct tctcgtttct
    24841 cttccggcat agaggggctg aacacatgtg cctgttgcca catttccgat atacttttca
    24901 aatcgcacat tcctgctgaa atggcagcaa ggtaagcagc gccaagtgca gttgattcaa
    24961 ttatctccgg tcgcaccaca tcaatctgca gcatatcact ctggaattgc atcagataat
    25021 tgttagcact tgctccgcca tcagctttga gtatttttat cgtaacccct gccgactgct
    25081 gcatggcttc aagcacatcg cgggtttgat aggcaagcga ctccagcgta gcccttacaa
    25141 tgtgtttttc ggtcacgccc tgagtaattc caagaatagc gccccgggct ttcatatccc
    25201 aatatggtgc acccaagcct gcgaaagccg gtaccacaaa tatatcaccg ctatcgctta
    25261 acgactgtga tatggtattg gtttcggcgg cgtcacggat aaggtgaatt ccatcgcgca
    25321 accatttaac agctgcacct gcgataaata cgctaccttc tgcagcataa acaacggaat
    25381 cgttaacttt ccatgccaca gttgaaagaa gtccattttg tgaattacag ggtttactcc
    25441 ctgtattcag caacataaag caacctgttc cataggtatt tttaaccatt ccttcatcaa
    25501 aacatgcctg cccaaacaag gctgcctgct ggtctcctgc aatcccgcca atttcaatat
    25561 ttcctttacc ggtaagtccg gttttcgcaa taccaaatgt accgctcgaa gcaactactt
    25621 caggcaacat agcagaaggg atatcgaaaa tatcaagcaa ttcattgtcc caacacaact
    25681 tgtttatgtc gaacatcatg gtcctcgaag cattgcttac atcagtaata tgccttttac
    25741 ctcctgtaag attccaaatc aaccaagtat caatggtacc aaaaagcagg tcgccgtttt
    25801 cagcagattt cctgacaccg tcaacacttt cgagaatcca ccggagcttg gttgcagaaa
    25861 aataagaatc aatcaccagt cctgtttttt gtttgatgat ctcttccatt cctattgatt
    25921 tcagataatt gcagtagtca gcagttctcc tgtcttgcca gatgatggca ttataaacag
    25981 gttttccgct gtgtcggttc caaacaaccg tggtttcacg ctggtttgtt attccaagag
    26041 atgcaatgga tgaaggttct atccccaaac gatggatgag cttccgtaaa acaaacagtt
    26101 gcgcttcgag tatttctacc gggtcctgtt ccacccaccc gggtctggga taaaattgct
    26161 gtatttcctg ttgctctatt ccaacaatcc ggaaccgttc gtcgaaaagc accgttctgg
    26221 aactggttgt accctgatcg aatgagagta tataagtttt gttatcgccc atgatacaga
    26281 atttttcgtc ggagccactt tataacatca tcatacacct cgttgcgatt ggtttcgttg
    26341 agcacctcat gccttgcccc ttcgtacagt gtaaaccgca catcggtcat tccgcaatcg
    26401 agaaaagcct tgtgaacttt cgaaactccc ttgcccttag cgcctaccgg gtcgttgtcg
    26461 cctgatataa aaaacacagg aagctgcttg ggcatattat caaggttttt tcttttacca
    26521 ataaataaaa gcccatggaa caggtcgctg aaaaatccgg cagtaaaaac ctgtccgcaa
    26581 tatgggtcgg caatatattg ttccacaatg gcactatcgc gcgaaagcca ctgaaatgga
    26641 gtactgtagc ccttgttaaa gctgccgaac gacaaattat cgaggaatct ggaacggtgc
    26701 tttttccctt taatttttcc gattatccag gcaataaaac tacctgcata aagaataagg
    26761 cttgggttct gccctgttcc cgacaatatt actccatcaa tttggttgcc atgcagacaa
    26821 atataggttc tggcgatgaa agaacccatg ctgtgcccca gaagatacga aggtttccct
    26881 gggttttcgg cgcgtgcaat acatatcatt tcgcgaatat cactggtaac tttacaccac
    26941 cctttggtat cggcaaaata ccctgttttt tcaggggaac cagcagttgc tccatgcccc
    27001 cgatggtcgg gggcatacac cacaaaacca ttggcattca atgctgaagc aaaattacag
    27061 taccgaaatc catgctctgc catcccatgg gcaatcacaa tagaagccct aatataactg
    27121 gtatccggaa tccacttatg gtaaaaaacc gcaattccgt cggaggttgt aaacgatttt
    27181 ttttcgtagc tcatatcgaa ttaatataaa tgttcgtaac ctgaaagctt aaacatttca
    27241 gcaagggcac gtatctccat gcgcatggcc ttgttcagtg gcacaccgct caacgaccgt
    27301 tcctgaagat ggatatattc tttttctcct gccgtataaa ttctttctgc tcccggcatt
    27361 tttgccgagt ttcgcaactc tctcagaata tttccggtag tagatttaaa ctcgcttacc
    27421 tcagtaaaat gcccgatatt gattgcaata aagaaatgcc ccaatctgta aggcactttc
    27481 tttccgttgt cgaaaccagt gagagcattc ataaatacac cgccctgcaa tgcagccgaa
    27541 agaatttcga caacagttgc gtagccgtaa cctttgtacc cacccgtttc ctctccaatt
    27601 ccacccaatg gtgtgagggc tgcattgcct ttggtcagat cggttaacac ttctgcaggg
    27661 tcggttttac tttctccgct cctgtcgatg acccatcctt tgggtagctg tttgccgttt
    27721 ttggcataaa attcaatttt tccgcgctga cttactgagg ttgcgcaatc gagaaaaaat
    27781 ggaaaatcct catcagaggg aacaacaaaa gtaagcgggt tggttcctag catattatct
    27841 ataccaaaag tgggggctat cgaaggcctg gcgttagtac ccgtaattcc gatcatattt
    27901 tcggctgctg ccatcattcc gtaatatccg gcaataccat aatgtgagct gttacgcaca
    27961 gctaccatac ccattccgta aacccgggct ttttcgattg ccattttcat agccgccttc
    28021 gaaaccacat gacccatgcc attgttagcg tcaagcaccg ctgtggtgaa agtgtccttg
    28081 actacatcaa ttttggtaac cggattaagt attccatcac gtatgcggtc gatatatatg
    28141 ggctttaggc ggttgattcc atgggaatcg ataccaaatt tatcagcttc gatgagaaca
    28201 tccgacactg tttcggcatc atcagcaggc acacctgcag ccataaggca ctctttcata
    28261 aacatttgca gggtatcgaa acctgcaaaa actacatctt cttccatcgc atattggttt
    28321 atgcgaatgt aataaataag ttgcctcgat ttatttatta actccaaatt aaaattaaaa
    28381 cataaaaatt aattttgcag taaaccatac gaggctgaaa aacaaagctt tcatattaac
    28441 agcagtattt tgcgttctgt ttgtgtacac ttcaaccgca caatcaggcg atgctctgct
    28501 ggataaatac gcccattaca gggcaagatt attcgacgat tttgtagtgg ttagtcctga
    28561 tgtcgaagat tttggtgtga atatacctgc taccgaccgc gtgctcgata cactggggaa
    28621 acctgtttat atcagctggg gcgatgggaa ctgtaatttc aaccactatc tgggttttct
    28681 tgccacggaa taccgccttc ttaaaaacaa tgggcgggat tatcaggaga cctatgagat
    28741 gttgatatac gctttgcttg ccatagaacg cctcgacctg tactcggaat acacacttcg
    28801 cctgcacgaa aacaaatcga agcttgttga cggcgacaca atacgcgaat atattgaata
    28861 tccctccgat atcaacggct ttctgatacg cgatgatgta agcctggggt tctggtaccg
    28921 ttattcgaca cactttaaca ttggttttgg caaaataaat aagactaaag acggtacaaa
    28981 tacctatcag tcgattttca aaatgggcgt tgttccaaaa gaagaaatga gtcaggataa
    29041 cattatccgt atgcttcacg cacttgccat ggttaaaatg cttgttgatg acgaacccca
    29101 gtatatggtc agtgttaatt tcatcaacac gcttatcccg gagtacttag cgagtaaaca
    29161 gatacttgct gacgacagca tttacatttc gcgctgggtt gaagatctgg ccaatcggct
    29221 tatcggacaa atgcaacacc cctaccccga aaaatcgctg tgcctgaagc cctgttgcgg
    29281 aatggcaaaa gtgagctcca accatttttt atcgctgata tccactccat ggtatatcac
    29341 caatcctgtt accaacgaac tggtggcaga aggttcgggc gaggatatgg gcttatggat
    29401 taattcatat ggcgttgctg aggcggggaa tgcgattaca agtactcaga accatcatct
    29461 tggaaagtcg gcctatggcg tcaatcctta tctatttaaa gctttattat tcaagcagat
    29521 gcgcttgctt cccggaggca gttttccgct gccaaaaagc atcgatgatt atatgctccg
    29581 cgatttggca gtaatagctg atatcaactg gaaccgaaaa tcaaaaaatc tgttctacgc
    29641 tcttcgcgac aaaaggaaac gtttaaccta tgaacatcag ttgctaatac ttaatattct
    29701 gcatcctgaa aaatacaagt cgttgtattc accgggtacc cgatatttcg aggaggacaa
    29761 gctgtactat cgcagtttgc ttgaagcagc tcctgccgca ggccctactt ccgttcagag
    29821 taacccggag tactccgaat tatggaacag ttcgtcgcga atgatttggc ccggtaaagg
    29881 agctcctgat cctaccaacc gctgggattt tgcaggactc gattacctgt atctgcataa
    29941 tctttaccga ctggtttttt gccctgacaa ctattcatta cccgaaaacc gcatgaaacc
    30001 acaggataaa aagacctacc tcaaccgcaa ttctgctgca ccgggttacg atgccgagtt
    30061 tttcttcagt gccccgaaag cagtccggca caaaaaggaa taggaaaact aaacattgtc
    30121 aaattttatt gattgaaacc tgccttttcg gtgcgtaatg tcgcctaaat ttatatcttt
    30181 aagccctgtt ttttattatg aaactgtcgg taataattgt caactacaat gtaaagcact
    30241 ttctggaaca gtgccttcat tcggtttttt ctgccattgg aagtatcgat gcagaggtta
    30301 ctgtggttga taacaattcg gttgacggct cttgtgcttt ggtgaaagat aaattcccta
    30361 cagttaagct tattaccaac aaaaaaaatt acggtttttc ttacgccaac aatcaggcta
    30421 tcaagcaagc gcagggtgaa tatatattgc tgcttaatcc cgacacagtg gttcaggaaa
    30481 acactttcac ggcagtgatt gggtttatgg attcccatag cgatgcagga gggcttggcg
    30541 tgaagatgat cgacggtaaa ggtcgctttc ttccagaatc gaaacgggga ttgcccacgc
    30601 cagctgttgc cttctataaa atattcgggt tatcgaaact atttccaaaa tcggaacgtt
    30661 tcgggcgata tcacctatca aatctcgaca aagaaaaaat acacaaagtg gatgtcctaa
    30721 gcggtgcatt catgctgttg cgacacagtg ttcttaaaac catcgggctg ctcgacgaag
    30781 attatttcat gtacggagaa gacatcgacc tgtcgtaccg aatcaccaaa gcgggataca
    30841 acaactatta tttcccggag accaccatta ttcactacaa aggagaaagt actaaaaaag
    30901 gaagcatcaa ctatgttatg gttttctata aagccatgat catattcgcc agaaagcatt
    30961 tcaacagaaa aaatgcccgg atgttttcgt ttctcataaa catggctata tatttccgtg
    31021 catttcttgc tatagcacac cggttcctat tgaggcttgt tgctcctgca ctcgacgcga
    31081 ttcttatatt tgcaggatat tactttctta caccgcaatg gagtctgctc aagttcgggg
    31141 aaattgatta ctatcccccg gagtttttcc actatgctgt tcccgcatat attatcacat
    31201 ggctgataac cttacttttc acaggaggct acgaccgacc gcttagggta atcaatattt
    31261 ttaagggact tgctataggc tctgttatca ttttactgta ttattctctt ctgccggaac
    31321 acctgcgttt ttcgagggca cttattctgc ttggcacagc ttacgccgcc attgctattc
    31381 cacttatgag agcccttctt cacttcacag gtatacgtgc attccgtatc gacatgcaga
    31441 agctgaaaaa agttgttatt gcagggaaca aagaagaaat tgtaagggtg agtgcactgc
    31501 tgaatcagac cgggctgaac aatcaggtgg ttggttttgt tagcaccatg cagacagaaa
    31561 ccaacggatt tttcatgggt agtatcgccc aaatcgacga aattgtacgc atttacaaaa
    31621 taaacgaagt aattttttgt gcgaaagatt ttccttcgca gcaaataatt aagtacatgc
    31681 tgttacttgc cgaacagaaa atcgattaca agatcgccgg acccgacggg gttaccgtaa
    31741 ttggcagcaa cagcgttcat accgccggcg acatttactc aatcaactta aacagcattg
    31801 gcagtccgaa aaacaagcgt aaaaaaaggc tgttcgatgt ggcgatgtca atagccattg
    31861 cgctcattgc tcccatactg ctttttttgg taaaaagccc catgcggctg ctaaagaatc
    31921 tgattttttg tttcttcgga ctaaagagct ttgtcggtta ctataaaaat gccggagtcg
    31981 cagggttgcc ttatctgaaa cccggtattc ttaaacccct cgatccgggg gtagaaacag
    32041 tgagccaaca aaccgccgaa agtgccaata ttttgtacgc ccgcgattac aacgtactgc
    32101 gcgatttccg cataattatc cgttccctaa ataaactcga tcgtgaagct tgaaaataac
    32161 cgaattcgac tccgtgctcc cgaacccgaa gacctggagt tgttgtatca ctgggaaaac
    32221 gataccggta tgtgggtggt aagtgaaacc ctggaaccgt tttcgcgatt tcagcttaag
    32281 gaatatatca gtcagtgcaa ccgcgatata tacactgcaa aagagcagcg cctgatgatt
    32341 gaaaccattg aatcgcccca ctgcgttggg attatcgata tgttccagct tgatccgttt
    32401 caccgaaaag cagcattggg catattaata ggcaacgaat accgtaagca gcactatgct
    32461 tccgatgcgc ttgagattta ttgcggctat gcttttgggt atcttcagct acatcagctc
    32521 tattgcgaaa ttattgcctc aaacacggta agtattcaat tgtttgagaa atcggggttt
    32581 cgctgcgtgg caaggttaaa agactggagg cgcattgggt cagactacac cgatgttttt
    32641 ctttatcagc gattacaacc cgacaacaac cggtaatcac acattatatt tcccttttaa
    32701 aaattaaggc aaccgcatac acggcaatgc cttcttcgcg acctacaaag cccagttttt
    32761 cggtagtggt ggcttttatg ctgatgcagc cgatatctac atgcatctcc gatgccaata
    32821 ctttgcacat atcatcggtg tactgagcaa tttttggttt ctgaaggcat accgtaacgt
    32881 cgatattgcc cgtttcgtaa cccgcttcgg taattatatt ataaacctct gccagaaggt
    32941 gccgactgtc gataccctta tatttggagt cggtatcggg aaagtgaaaa ccgatatctc
    33001 tttttccggc ggcaccaagc agtgcatcgc aaacggcatg aataagaaca tcaccgtcgg
    33061 aatgagcgac acagcctttt gaatgaggaa tatttacacc gccgagcgta agtgtaagac
    33121 cgggtgcaag ctgatgcaca tcataaccga acccgactct tacattgtgc atttttactg
    33181 cgcttagtta ttttcgtcgg agagtccgtc gatatcgaag gtaagggtaa aacggagtgt
    33241 attttgtaat gggtgttgct gtgctgttgg tatgaggtac gaaaaatcga gtccgaatac
    33301 attcagcctc agaccggcac ccacactgaa atatttacgg tttcccttgt actgatgctc
    33361 gttgaaataa ccggcgcgca atgcaaagcg tttgttgtac cagtattcca taccaaagct
    33421 ccatgcaatt tccgacagct cttctttgaa cacattcggg gtggtttcat cttttctgaa
    33481 tggttctgca attccagcag catcgtacca gctttgaaat atagcggcag gtaccgaaat
    33541 ctccgggttt ttcccataag caattacaat attgccgtta ttatcgtaaa catttgctcc
    33601 ggtagaatca agcagatacg aaggcggact tggaacgagc agtttattca aatcaacggc
    33661 aaccacaata ctgttgtatt cgttaatgtt cataaggtat gcaacaccgg tgcggaaatt
    33721 tgcaggcaga aaattgcgtt tttcattgga tgtgtacgaa attttagctc ctatgttcga
    33781 aatgttcaaa ccccacatga ggttggtttt catgtttccc agatcaatat tctcgctgtt
    33841 gtagaaaaac gatacatcgg ctgcgcccga attacctgca tgggtgtctt cgcccgaatt
    33901 attcgacaca ccgcccgtaa ggttgctatg tatgtaacgc aacgctacag ctccggaaaa
    33961 gttttttcca agcaaccttg aataagcaaa gtcgatggaa aactcattgg gcgtgaaatc
    34021 gcggatggtt ccaccgttta tatcagtgaa agtgatacct ccgagcgaga aataacgaag
    34081 cgaagctgca atggtgtttt ctgcatcaat ttgtttgtat gcggacaaat acgcgaggtt
    34141 catatcaggc accagctgac gcagccatgg agtgtacgat aacgacacac ccagttcctt
    34201 ttcgcagaaa gcatattttg ccgcattcca gtgcagcgaa tttgcatcgg gggtaacagc
    34261 aacaccggca tcacccatac ctgcagcccg gctgtcgggg gcaatcagca gaaatggaac
    34321 agcagtggta ataatgggaa cattatcggt acgaccccag atatctacat caggctcatt
    34381 ttgagcataa gaataattta atgcaaagac gcttgtcaac aatatgaaaa atcttacttt
    34441 cttcattttt aaacaattat ttatcatgaa acaaatataa gcattaaaca ataatgccaa
    34501 tcaaattatt gtgtttttta tatttttaat ttaacaaatc tcaattaata ataaacaact
    34561 tttcgatttg ccgtgcaata tttccgttgg gagctttcac actgatttta taaatgtaca
    34621 ctcctttacc aattttgtcg ccgtaatcgt cgagggcatt ccagcttatg ggctggctgc
    34681 ggaaaccatc ggaaacaact actacttcga tggttttcac cagtttgcct gcaactgtaa
    34741 acacctgtat cagcacgtcc atggggacat tgggttggtt gtgttcgaaa taaaattggg
    34801 tgttcgttga aaacgggttc ggataattga acacatggtc gataaccaat tcactgctgc
    34861 tggcaaccat aaaatcggta taagcaactg aggaattgtt aagcacatcc caaaccttaa
    34921 ggctgagcgt atggggtcct aattccaggt tggagaacgg atacagtatt ttcccggaag
    34981 tatagtcgtc gagattcgac tcatagtatt cgttcagcac aatggaatgg ctggtatttt
    35041 catcgagaac ggcaagtata tcgtgaccga tgccgctacc cacggtgttg atacccgagc
    35101 tgtcggaaac aaaagccaac aggttggggt tttcgtcggt aattccaccg ataataaaat
    35161 cttcgttatt catatataat tcaatctccg gaccttcgtt gtcggcaata ttgctgtcgg
    35221 agcttccacc aatcatgatt tcggaataat tcccgtgggc atcaacattg ttgtcgtggg
    35281 cataataact tattttgcca ttcccataga agtaagcgat atcaaccgga acgataaagc
    35341 tgaatgtaaa ttcgccgttc acaacactgg tttgtccttt aaaaaggatg ttcatttgct
    35401 cggagaactc caggggtggt tttccatcgt tgccgcgcgt ctgaaactcc atatttttat
    35461 cgtacacaac agggaacagc aaaccattaa aacccgacat tttatttcca tcttcatcct
    35521 gaatatagcc tgtaacaata accttgctgc gagccccaac cgtatcggcg aagctgctca
    35581 cattaatacc gttgatagaa gtagtaacca cattgtattg cggcagggcc ggacgcatgg
    35641 ccgggtcgcc gagcaacaca aaatttcgtt tatttgtatc gctgttcaac tcgtttttgg
    35701 ttcggatgat aatctgcccc atggtatagg gtttcaggtc ggcatctttc acaaagaaat
    35761 aattataaaa tgctttgttg agcgatgtgt tggcaccggc ctgcaccaaa cggctggtag
    35821 tgaacaatgc tatgccgcca ccgtgtgggt tgagcaacac ctgttcgcct gccgacacca
    35881 gttcgaaatc gtcgaaacgc gaaaattcgc aggtagcagt aaaaaatatt gcgtaattgt
    35941 tgttgttgac ccatgaatta atctgcgaca aatcaacaac cacctcatgc gcaagcgttt
    36001 tggtgtttcc atgaccggta taattgaata tcagcgtacc cttttgtatc ctgttatcga
    36061 ttgcctcgtt cacctcaggg tagcgcattc cctgaacggt gctaacctgt tcgtatgagt
    36121 cgagaaaaat cttatcgaca ttatataccg gatagtttgc cctgacaata tcggcaattt
    36181 cgttacaatg aatctggtgg atgtcgtaat cttcggcatc atcaccgaca aatgtaaccg
    36241 tgttgcgcca ggaccccttg taggccgatg aaatgtatgc aataatttta tttacaaccg
    36301 tttgtgcttc gctgaccgat tttgcaggaa tccttcctat accaatatcg gagtcgtcgt
    36361 agccattcac agttccttcg ttggggtcga gcatcacata gaagtcgtcg ctcacaaacg
    36421 caccactggg cgagaacgaa ttggtggtct gataggtagg aacaaaattg ccattaaccc
    36481 ctgagggagc tttgttgtcg aagctgccat cgccgaacaa caacaagttt tcgggaattt
    36541 cgtcatcgct gccagccctg tcgtaaaaca ttttaacgaa atcgcgaatg gcagacacat
    36601 cggggcatcc cgacgaaaat tcattataaa ttattgcggg atcaaccaca atacaactca
    36661 tcccgtcgtt caaggtatgg aggtcggcaa gctgctgggc ctgtgccctg aaacccggat
    36721 aggtaacaat aataaggtcg ggattagcca gggcatgcag gttctgattt gcaatcagtc
    36781 ccagtccgtc gccttcggtt accggacttt ggaagctgcc gctggggtta aacgcaatgt
    36841 attcgtgcag ggtttctgaa ttgcggttaa attttgtttg cgttccggca agtacagggt
    36901 ttatctgaac cgcattcacg gggtcggtaa tatcccaaac cacagtattg gcgttggtat
    36961 ttccgagaac aaactgagct atactgccgg ggtcgaccga gttgatatcg cggaaaataa
    37021 gaaaatcgga cgacatggta agagaacaac ggcccgaagc cctgatataa ttgagccaac
    37081 cctcggaatc gctggtagat ttaatatagt cgatccgata actgatatcg ggtcccgctg
    37141 caaggtgcga tatagaggca atttcttcct tggcatacgt ggagctgtag tttccgctta
    37201 catatccaat cagtgtggtg tttaccagtg tgtttgcaat ataaatttta aaattgctgc
    37261 ttaacgatga acgcgctgcc aggtaaatgt cgatatcgag cggctcactg gtaataaggt
    37321 tcggaaaatt ctgagtgaac tgataggatg tatagaagtc gaagtgacgc cagaaccagg
    37381 ttctgcccat gccccgcaca ttgagagagt ccttttcgag aaactttacc tcgttgtagg
    37441 agttcaccac tgcattgaaa gttgcttcgg agttaattac ctgagggctt ttccaggtgc
    37501 cgcggtctga aataaaataa tatgcatact ccgaataatc gtgcttttcg tgtttaaatg
    37561 actgcgaact gttatcataa taaatgcggt cggggccgtc ggcataaaag aggatgtaat
    37621 cgccggcatc gaacaccgag tttgcgttag catccacctt catcatatct ctttcgggca
    37681 ggtcgtcgta gcggacttcg ttattctttt taggaagcat ttttccaaac ccgaacactc
    37741 cgagtgttga aaaattgcta aaacccattt gcgtcaattg ttcataagtc agtttataaa
    37801 taccggaagc atccagcttg attttatacc atttcccgct gctcaatttc gacgacgaag
    37861 cgtaagagcg catatttcta agttcaggct tagcaacagc cgtagtttgc aaggtaaacg
    37921 atacaagctt ctcaatgcgg ccggtaattt tattcctgcg taagggtatc aattcaaaag
    37981 ataccgcata ttccttgcga aagcaagagg taaaaacctc tatgttaatt tcttcattaa
    38041 ttttatcaac atctgttaaa aagttgagca ccgaatcggg taacgcttcg taaacagcgt
    38101 taacaatttt tggattttta acagaaattt cgctttgggt tctgattttt ttgtagtaaa
    38161 cgggaagaaa atgttcattt ttttgccctg atttagaaaa acttaaaata atttgcggtt
    38221 tattttcgtt atttaaacta aaaataggat tgttccatcg aattgaatcg gaaaattcaa
    38281 cagactgtga aaatgcgtaa acaccaatta acaagccaat tacagataaa agcaacaact
    38341 tatacgacat acgtggtaac attaaaaaac aaattttaaa aaaagatttc gtcatttttt
    38401 caaaaacgcc aaatttatac taatattgta gttgatattt tttttgaaat aaatttattg
    38461 tataattgca aattgacgtt caattgttat agcatgataa gacgtatact ttttatctta
    38521 agtattttgc tgacagggat ttacggagct gttctcggac aggatgccca tttttcgcaa
    38581 ttctatgcca acccattgca tctgaaccct gcattcaccg gatcggagag atgtccccgc
    38641 gtttcgctcg ggtacaggaa tcaatggcca gccttagggc caacatacgt aacttacagt
    38701 gcgtcgtacg accaacatgt aaatttcatg cagggcggtc tgggtttgca catattaagc
    38761 gatgtgcagg gcgacggcac gataaaaacc aatatcataa gcgggatgta ttcttacacc
    38821 ttgcccgttt cgagaaattt tgccattgcc ggcggtttcc aggcatcgtt tgtgatgaaa
    38881 accattaaat gggactttat tttcccggat atgatacatc cgttgtatgg tcctatctac
    38941 actactcagg aaaaccccga tcttgtaaat gacaataaaa attatgttga tttttcggtt
    39001 ggtctgattg gttttaccga aagaactttc ttcggtgtag ccgttcatca ccttaccgag
    39061 ccttcggaat cgttcctccg tggcagcgac gctgtgttgc cccgtaaatt taccgcacat
    39121 tttggaaccg acatacctat aaataataag aaattccgcc gtggcgaact ttcgttgtca
    39181 ccgcagctgt tgtttcacca gcaaggtcag ttccagcagt tcaactgggg tatctatgtg
    39241 aaccgcaagg ctatcgttgc cggattctgg ctccgtcaga atttcaactt ccagtacgat
    39301 tccttcatta tgcttgtcgg attccgtcag gacaacatcc gctttgccta cagctacgac
    39361 ttcactgtgt cgcgtttgcg caactctact tacgggtcgc acgaggtttc ttttgcatat
    39421 attttcccat gtcacgaaaa agctgcgaag aaacgtgtaa taagttgccc ctcattttag
    39481 ttattattga tagaaatgtt taatatttta taaatatgaa aactaacttg tttacatggt
    39541 catttattat tttactcatg gtagcatttg tttcctgcaa aaaagaggtt tcgaatacta
    39601 cagggcgtaa ttataatgac cccaaacacg gaggattcga aaaccatccg gaatatgaac
    39661 aggaaacagg ccccggtctg gtgtttatcg aaggtggttc ttacgccatg gggcgcacag
    39721 aacaagacgt tatcttcgac tggaacaatg caccccgccg cgttaccgtt tcttcattct
    39781 atatggacga aaccgagata aggaacatcg attatctcga atacctctac tggctacggt
    39841 atgtttacgg tcaggaaaaa tactttatat ataatgaagc cctgcccgac accctggtgt
    39901 ggagacagcg tctggctttc aatgaaccca tggtggaact ctatctgcgc caccctgcat
    39961 acgaagatta tcccgtggta ggtgttagct gggtgcaggc ctctaagtac agcgactgga
    40021 gaaccgaccg tgtgaatgaa gtaattcttg ttcgcgaagg catcttcgtc gacaatctcg
    40081 ttccacgtca gggagtgaat cagtttaatc ttgactcata ccttttcagc tcctcaagct
    40141 acgatattac ccttgaattg cctgtttcaa aagacaataa aaacaaaggc aatacgaatc
    40201 acgacggtaa agttgacaat ctttacagac ccgccgatta tttcgatacc gacaataaaa
    40261 acaaacaaca gcaaaatgtt accgaaacac gcagaaacgt gagaacggaa gacggcattt
    40321 ttctgccgcg ctaccgactc ccgaccgaag ccgaatggga atttgcagcc cttgcacata
    40381 tcggtaacac ttacaacgaa cgtatttaca accgcagact ctatccctgg aacggccatt
    40441 atatccgcaa cgacagccgg aagggcgatg aacgcggaca gatcatggca aactcgatgc
    40501 gcggacgtgg cgacaacatg ggggtagccg gtgcgctgaa cgacaaagcc gacatctcag
    40561 ctcccgttaa atcgtactgg cccaatgaat acggtctgta ttgcatggcc ggtaatgtga
    40621 acgaatgggt aatggacgtt taccgcgaca tgacatacga cgacatggag gaattcaggc
    40681 cttaccgtgg gaatgtgtac gatgattaca acattactat tgaccctgaa aacggtgaag
    40741 taaccctcac tcccgaggcc tcaaactact tcaactatgt aggtcagatt caacgcaaac
    40801 ccgttagcga tgaagcctgc tttaatcgcg aaaactatac tactgccgac aaccgcaact
    40861 acctcgacgg cgaccttgaa tcacttatcc cgggcggtgc tgctaactac tggaattcaa
    40921 cggtagacta taaacccgat acactgaaag tgcacaatcc aattgacagt atcgacctca
    40981 ccaaacttcc gaaaggaagc agttaccgca aaacaggaaa cgatattgaa atattcctgc
    41041 ccaataaaat cgtcataagg gttgaccaga ataatgttcc tacctacacc ctgcctaatg
    41101 gttacagaat aactcaggac agcgggaaac aggttgttat tctgcctgac ggatcaaaag
    41161 tattgcccgg aggtgttatc gaattcaccg acggagagcg attgctgcca cgcgatattt
    41221 ttgcctacac cggatatatg tacaaggatg gaaactgggg gcgcgatgga gaatggggtg
    41281 caggacaata tgataaaatg aatccgaaag gcaacaaagg cgacaatatg aaatcgctga
    41341 ctaccgaccg ctcgcgagtg ttcaaaggtg ggtcgtggaa cgatagagca tattggatgg
    41401 tgcctgcgaa ccgacgattc ctcgatgaaa aaatggcccg agccgacctt ggcttccgtt
    41461 gtgccatggt gagggtagga tcgcctgtgc agggaaaaaa ataggatggt attaaaaact
    41521 aaataaagcc cctcttatgg ggcttttcat tttataatgg taacgactga acaactttac
    41581 aaaatttttc tcaaaacacg ttctgtttgc accgacagcc gaagggtaac tcccggatcg
    41641 gttttctttg ccttgtcggg cgaaaacttc gacggaaaca attttgccgc acaggccatc
    41701 gaaatgggct gcgcttatgc cgttgtcgat aaacctgcag tggcattgaa cgacaaatat
    41761 ctggtagtga acaacgtgct ccaggcattg caggatcttg cacggcatca ccgtaagcaa
    41821 ctccgaatac ccgtgattgc cattacaggt accaacggta aaaccaccac caaagagctg
    41881 atatctgcca tactatcgaa aaaattcagg gtgaatgcaa cccgcggaaa tatgaacaac
    41941 catattggtg ttccgctcac cattctttcg atgaaccaca ctaccgacat gggtgtggtt
    42001 gaaatgggtg caaaccaccc gggcgaaata gctctgcttt gcgatattgc ccagccaaac
    42061 tacggactga tcaccaatat cggcaaggcc cacctcgaag gattcggctc agtagaggca
    42121 attattaaaa caaaagccga actctacgac tacctcatgg gtcatgaagg aataattttt
    42181 ctgaacaacg aaaacccgat actgaaaaaa ctcgtgggca gttacccaag cgtaaactac
    42241 ggcgtttctg acgaatcgtt ctgctacgga aaaattacct ataaagaaat atatgtgggc
    42301 gttgactgga tatgcggaca gaatacaggc ttggcctact cattccttat tggcgactac
    42361 aatttcgaga acatcatttc ggcaattgcc atcggcaact acttcaacat tgccggaagc
    42421 attatcgata aaaccgtgag cacctatacc cccagcaaca accgttcgca gtggattaaa
    42481 ggaaggcgca accaattgat tctcgattac tacaatgcca accccaccag catgatggcg
    42541 gccatcacca acttcaacaa ttacgacagc gagaaaaaat gccttatcct cggcgacatg
    42601 ctcgaactgg gagatgtgtc ggaaaaagag caccaaaata ttattgaata tatcaacgca
    42661 tacccttaca gcgacgtttt tctggttggc aacacttttg gccggtttaa agacagttac
    42721 aattttcatt ttttcgaaac taccgaaatg cttgccggct atctttcaaa aaaccctccc
    42781 accggctacc tcatactgct gaaaggatcc agaggcatga aacttgaaaa atgcgtcgat
    42841 tacctctagt ttattaaaac tggcttgtta atcattcctc aatttctgtt gctaaacatt
    42901 gtaaaaaatt tttatttttg cttcttgttt aacttttaaa tttcgattga acgatgaaga
    42961 attttttact ttcaggtgta ttgcttgttt tttgttttgt agctgcagca caaacaaccc
    43021 tgcctaacat gaattttgaa gattggcagg aaaatcatta cagtagttgg tatccttcca
    43081 atttttactg ggagtgtctt cccacaacaa tatggtcatc gggaaatgca gcttctacac
    43141 aggtaaatat tttccccact taccgcacta ccgatgccca aagtggcagc tatgccgcct
    43201 gccttgaaac taaagaaatt ttcacccaga cagcctccgg aagtctgttc accggctggt
    43261 ttgaacccgc tatgttaagt tcagaagccc atttcggtgt tcctttcacc ggcaagccca
    43321 ccgcattccg cggctggtac aaatacactc ccgccaacca gagcggtggc accatcgata
    43381 cctgttcgat ttatgccgta ctttcgcgct ggaatggcag cgagagggta gaaattgccc
    43441 gcgccgagtt gtacacttac accacggtga gcagctatac gtatttcgat ttaccgttca
    43501 attattacaa ctcgttgata cccgatacga ttgccgtgat attttcgtcg agcaagcatg
    43561 gcgatatatt tcagggaggt atcggcagca aactgtacat cgacaacatc gatttgtact
    43621 acgatttgtc gtcggtgcaa ccaaaccacg ccggagtaaa cgaagtgcat atcctcttcg
    43681 attcgtatgg taaaaacatt tcggtagaaa ggaattacgt gaagcccgca agcatagaat
    43741 tgtttgatat gaccggcaaa aagctgtatt cctatagcat tgagggactg aaaagagaga
    43801 tgaatttcga cggattgcgc accggaattt acgtttgtcg ttacagcgac aatgccgggt
    43861 tggttaaaac cgtaaagatt tattttaaat aagataatgc aaaaaattac ccttctcgct
    43921 gttttagtgt atctgagtgc cagtgcattc agccagacca tttcgttcgg gcctaaactg
    43981 ggaatggtag ccggttcgcc ggtgccctac agttttattc ccgaaggggc taaaggtgct
    44041 cccaagccag gtcggaatgt aggcttgttt gtaaaattcg attttacgga gaactatagc
    44101 ctgatttatg aggtttccat gacgcgcaag cgcaccacct tcgaaactcc gcttgacagt
    44161 gtagcctatg tcgatcgcat tcagcacccc ttgtttcctg acgtagtatt cgaagtggag
    44221 acttttttca atggaactgc agtgggatgt ttcgacaact attatctgga acagtcactc
    44281 acgcatcaga tgcgcattac cgaaaaaatg aacgggttgt tcggacttta cacggcatgg
    44341 ttgcagaaga gcaataccta tgcaaaagga atcggcaggg ttgggttcag tccggatatt
    44401 attgagcagg aactcgatta ttcatcaaaa atgcggcaat gggactacgg tttgaaagcc
    44461 ggcatcggct atgaggcaag caagcacatg gatattgacc tgaaactgag ttatggtttc
    44521 gagtcggttt tctgcgatag cttctctatg ctcaactaca ctgtaaataa cacatttgta
    44581 gagctttcgc tcaattacaa gttttttata ttacgccagc tgaattcaca gatttagaag
    44641 gctcttccca aacgtcattg cgaacgcagc gaagcacctg tgctgagcga agtgnnnnnn
    44701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctaaca ttcgcatgac gggctcttcc
    44821 caaacgtcat tgcgaacgca gcgaagcact tgtgctgagc gaagtgaagc acttgtgctg
    44881 agcgaagtga agcaatccac caatacagag tcatgatttt tatagcccgt agaagtacct
    44941 gtgcccgtcg aagcacagat tgcttcatgt tcgtacctca cattcgtatg acgggctttc
    45001 aaacctaatt aaagaaatct tcgatttgca atatagcttc ggggagtgca aacaccacca
    45061 ccttatcgtt ggctttgatc agggtgtttc cgtttgctat aaaactttta tcatcgcgca
    45121 caacaccacc gataatggct ttcttcggga agttgatgtt ccgcacaata tctccggtaa
    45181 tttttgcacc cggctgaacc acaaattcga gtacctcagc atcgctgccg gtaatacatt
    45241 taaccgaaga aacttctgca ttaagtgtag atgagtaaat atagcttgcg gcactcattt
    45301 ttttattgac gattgtatcg atccccatct gggttgccac atcaatgaag tcgatgtttt
    45361 ccacttcggc aatggtcttt ttcacaccca tcctttttgc cataatgcat gacagaatgt
    45421 tggtttcgga atttccggta accgcaagaa aggcatccat atcctgaatt ccctgttctt
    45481 cgagaaactc aaggcttctg ccgtcgccgt ttattatcag gcacttattt aactcgttgg
    45541 caagcatctc gcacttatcg gaatcaatat ccaccatttt aatgttgagg tgcgattgca
    45601 ttctcattgc caccatacgt ccgatacggc tcccacccag tatcatgatg ttcgaaattt
    45661 cgagtttttt cttccctgca tatttcaaaa tgcttacgat tccactgggg ttggtgataa
    45721 catacacaag gtcgtttacc atcagtacat catttccgtt agggatgacg gttttattat
    45781 tgcgcgaaat tgccaccaca cggtaatcgg tcgatttggt catggcatca gcttcaataa
    45841 gggttttatt aacaataggc gcatcggaat cgagtttaac cacaaacagg gttagttttc
    45901 cccctgagaa gtcgaagatc tcggaggttc ctgtttgttt tatcaaccca ttaacctctt
    45961 tggcagcaat atattccgga taaatcagtt tgtcaatacc caatcccagc aagtggggtt
    46021 tatatttggg ttccaaatac tccatgctat cgatacgcgc aatggttttg cgtgctccca
    46081 gttttttagc aagaatggca gcaatggtgt tgttttgctc ggtaacagta actgcgataa
    46141 aaaggtcagc ggttttcacc ccggcttcga tcaaggttcg aatcgaagtg caggacccgg
    46201 taacagtgag cagatcggca tcggattcaa cttctttgag ccgtttttcg ttattatcaa
    46261 ttaaaataat atcgtgatct tctttcacca gcattttcgc cagataggcc cctacttcac
    46321 ctgcacctgc tataataatt ttcatctgta tagattttta tttgtcagac ggcattttgc
    46381 aaaaattaag attcattttg actggtgata tttttaacat gcccgacttc atattattca
    46441 atttttcacg cttactttct gatttgcaat tatatcaata atcataacaa aaccgaccgg
    46501 ccaatgaccc tgcaaaatta ggcattattt gtcaataata aaagcaccct tgttttttac
    46561 atcaaaagca ctcaggccga tatctgctgc ctgctgcaac aacagcttcc tccggttgtc
    46621 gcttacagat gcatcaataa ccagttggtc aaatttcaac tgtctgctaa gttcatacac
    46681 atcgcaactt gcttcatcgg tgataattac taaatcgacc gcaatatcat ttagggtttc
    46741 aggtgatttt gcatatgtgg ctcccggtat atatattttt gtatcgtaga agccgataaa
    46801 attgtttttt acaaaaaccc ggcggtttcc aatctgtatg atattactca gcaagcgggg
    46861 tgccaacata tcctgcaagc atattgtttt ctcctttcct gctccaatag tattccagta
    46921 gttagccata gtatatttac ggctcttttc actgatagga tccagcccaa gaaaaagaat
    46981 attatctctt ccatcgataa tgttaatcgc agtagtgttg cgtaaattat atacaataat
    47041 tttacgctga ttgattgtat gcaggctttt tgtaatacct atcgcaacga aaacaacgat
    47101 gagcgagagc gtggcaaaaa gatatcccga tctgcggaat tcgaggaaca gcaccaccaa
    47161 tgcaatcaga aaaagaagga taacgaactg tgtgttgctg atataaattc catcaataac
    47221 cgaacccgga aggctttcga taaatgaaac agagctgttc atgagaaaaa taactttact
    47281 gaaaacccat ccaagcgcac tacccagaaa accaatccac gagaacatgg caatagcaat
    47341 ggctaagtat attgctactg taacaagggg aataataata aggtttgtaa gtaaaaagta
    47401 actcgggaac tggttaaaat aaaacaatgt gaaaggaatg gtgccaagct gggctgccac
    47461 cgacaccgac aacagcgacc ataccttgtc ggccagccgg ctgcgtactt ccacaagccc
    47521 gtaaattttc ggatagaagt ataaaataga gaaaacggct gcatacgaaa gctgaaagcc
    47581 aatctggtgc agcaccaatg ggtcggctag gagtgagaag aatgctgccg cagccagtat
    47641 attgaaagaa tttgcgtttt tccgaaacaa tcctgcactg gcaaaaagcg aaaacataag
    47701 tgctgcccta actacagaag gcgaaagtcc ggtaataagg gcataccccc atatcagcaa
    47761 aattatcagc agggtcttaa tccactgcct acgcttgctt ttaatgaagc cgagcaactg
    47821 agatattacc agatatacca cacccacatg caaacccgac acagcaagca catgcattcc
    47881 tcccgaaacc gaatagctct gcctgatttc atcgctgagg tatgatttat ttcccaaagt
    47941 aagggctgta gccacagcaa gttcatcgcc actgagtccg catttacgaa agctttcggt
    48001 tacagagtta cggatatttg ctgcaagaac aaacaatgac ccgctcggtg gttcttcaat
    48061 tttcttccat tcatctgatt ttatataatc ggtgtattga atatattgat accacatgta
    48121 ttttttgtaa tcgaattctt cggggttacc ctgattctca aattgtttca gttccggtga
    48181 caaaataagc ttgtcgccca ccccaagaag cattgctgca gagtcggcag ggacaaacac
    48241 cataatactc ccctgccctt cgtaccatgt atcaaccatt ttcacagctt cgagtgcaat
    48301 atcgagcttt accgttttcg cgcttacctg tgggtcggag ctaaccttgc caataatctg
    48361 ccctttgtaa ccggaaagcc ttgaatcgtt ggcagtttct gattttagtc ccgtaagcgt
    48421 gagagcgctc agacatataa atagaaatat ggtaaaagta aaaacacttt cgggtggctt
    48481 ttctttcagg gcataaagcg cgatcaatac aagcagcaag actgtaatag caacaatgcc
    48541 gaatatacat ggaatggtaa aatatccggc gagaataatt cccgcaataa acggaatcaa
    48601 caaccttaca aaaggatatt gcttcaggaa attcaagcag tcaaattatt tattcatttt
    48661 ctcaaaagcg tcaatcagct cttcgtctga taatccgagg aacttaatgg aggcttcggc
    48721 atcgtcggaa agactgtgat ccgggtactt ctgaataaac agctcataat aatgccttgc
    48781 tttggctgtg tcgttcaggt aattatgata ggtataaccg agatagaata tagccaaagg
    48841 ataatcattg cactgcatcc ccgatttttc gacagtagta agatattcta ccgcattatg
    48901 cggctggttc aggctaagtg caatttccga cgctttaatc aggtattttg ccgatgtcgt
    48961 atcgttaggg aatttgcggg aataattaac gtatttccca atcatttgtt ttgctttttg
    49021 agtatcgaag tttttcgaca catacagctc tttctcggtt ttatcgatat cggcactgag
    49081 ttccgctctg agtgcttcgc tgctttgttc gggtttgttc ttcggtttgc aggaaacgac
    49141 caacatgacc atgactgcaa gaatgatata cgtatttttc atagtacact gtattttatt
    49201 cgtaacaaga gtataaacca cacaagctca ttggcaaaaa taaacaaacc gtggttttac
    49261 ggagtaacaa ttttataaaa agttgatact tttcctttgg agatattcag gattttattt
    49321 ttgagaaatt tcttgcgtat cggggaaatt ttgtcggtaa aaagcacccc gtcaaggtgg
    49381 tcatattcgt gttgcaccac aatggctttg aagccatcga gttcttcccg tacaaaatta
    49441 aaatctctat cgtagtattc cattgtaagc cgtgtgtatc gtttcacgtt ttcgtggatt
    49501 ccgggaatgc tcaagcaacc ttcttcccat agaacagttt cggtgctttt ttccactatc
    49561 tgcggattaa taaaaacccg cttaaaattc ataagctcaa cattgtcttt agccacaggc
    49621 gtcgtgtcga ccacaaaaat acgcagagac ttgccaactt gaggggcggc aagtccaacc
    49681 ccatccgatt cggccatagt ttcgaacata tcgtcgataa atttctgtat ctccggagag
    49741 tttttcacta ccagctcggt tttccttcgt aacaccggat gccctataac atatatcggg
    49801 taaatcatag ttttgtattt ttcacataat ccatatacgc ctgcaaaatc aatacagcgc
    49861 tgatctgatc gaccagccct ttatttcgtc gcttcatttt tttcacaccc ccgtcaatca
    49921 ttgcctgaaa cgaaatctga gaagtaaatc gttcatcgta gtaaacaaaa tgtacatctg
    49981 caaaaagttt ttttaacctg gtcactacag gttcaatata tttaaccgca tccgatggca
    50041 aattgttcat attacgcgga tacccgacaa ttatcaagtc aactgactcc tttgccaaat
    50101 aatcctgcag aaacccggaa agctttcccg tttcggtgaa acccaaggct gtggcaatta
    50161 taccaagctt atcggttacc gcaacacctg tgcgtttttt acctatatca agtgcaatcc
    50221 agcggggcat ttttttttgc aaagttaaat ttttgttgca acagattaaa agttttcgta
    50281 actttaagcg tttttcaact aattgtaatt cggcatgaac aaaattctac tttttcccac
    50341 atttttactg gcaagtattc ttctgagcaa tgtatcattc acccaaacta caatttccac
    50401 ctataccaac gtaccattgc aggctttgat agccgacaca ttggtgaacg ggtgcactca
    50461 ggtgtcgaac atcactgtga attcaaatgg ggcatggggt tatttcagga aaaacaacag
    50521 ccagttcccg ttttcttcgg gcgtcatcat tgcatcgggg gctatatcga atgctcaggg
    50581 acccaattca agcagcagta tgggtaactc catcaattcg ggaagcgacc ctgatcttgt
    50641 tgaaattgcc ggcggcggct ccatttacga ctgtacaaaa attgagttca attttattcc
    50701 cgctgacgac acggttgagt tccggtatgt ttttggctcg gaagagtttc ctgaatacgc
    50761 caactcgaat tttaacgatg tatttgggtt tttcctttcg ggacccggaa tcagcggacc
    50821 ctatctgaac aatgctatca acatagcctt gctgcccaac gggcaaacag taaccattaa
    50881 caatgtacac aactacaact actatactgc tactcccgga tcgcagagca actctgcagg
    50941 gtcgtattac ggagccgttc agtatgatgg aaatactata gtattaacag ccagggcaat
    51001 tgtacagcct tgtcagactt accatataaa acttgccgta ggcgaccgtg gcgactcggc
    51061 ttacgacagc ggtgtattct tcgaggcaaa cagtttcgtt tccggcacca tgatcgaagg
    51121 cataaaccac agccagtttg gaggagaatc tgatttgtgg gaaggttgtg agaactatta
    51181 tgtaataagc agggcgcccg gctcaagttc cgctttgccg gttaccgtta acctttcggt
    51241 aggcggaaac tctactgcaa ctgccggtgt tgattacacc acattgccta ctgtagttac
    51301 aattcctgca gggcaaatgt cggataccat tttctacaat gctttcaacg atgctattga
    51361 agaaggtcac gaaaccatta tactgaactt ctacacaacc tgcccctgtg ggaatatgcc
    51421 ttcggttatt gccgatacca tctggattta tgatgcacag aagataaaag ggggtattca
    51481 ggatgtacag accaactatt gcggtgttgc tccgccaagt tctttaacat tgtacgcaag
    51541 ttgcaacctc gacacttctc aggtttccaa tgtatattat cactggagta caggttcgaa
    51601 taccaattct acaaccatta ccccccaacc gggagccact acttactatg ttacgatgtc
    51661 cgacgattgc gggaacgaag ttttcgacga aattacaata ctggtatcga gtatgcagat
    51721 atcgaaccag gttacaaccc ctgtaacctg tcacgatgca tgcaacggca cctatgtggt
    51781 taactgtgtc aacaacttcc ctcctttcac ctatacgctg gtaaatgccg actatgtttg
    51841 gttccccgac agtattcact cacaaaccac gggatctttc aatcatttat gcccgggtac
    51901 ttatctggtt accatcaccg atgatatcgg atgccagatt acaacagaat tcaccttgaa
    51961 caacctgcct ccgatcaatt actcggtggg tattaccaca ggtgatcaga attattgcac
    52021 cagccccggc cccattaccc ttacggcaga agccaatcag tcatcggcaa cttttgaatg
    52081 gttcaacggt tccggcaaca gcagcatttc ggtgacccca accgttggtg taaacaatta
    52141 ctgggtacga atctacgatg cttgccagaa tatgttcgag gataatgtta ctattactct
    52201 gtcacaaatg tttgcaacca cttcatctac cgacgacacc ggtgattgtg aagggaccgc
    52261 ctgggtgacc gtttcaaacg gcatcagtcc gtatagcttc tactggcccg acatcgatga
    52321 attcggctat ttgcaggaag atctttgcca tggcacctac gcagttaaca tcaccgacaa
    52381 tatcggctgt cagctgacaa aacaggctac cgtgcttctc aatgtttcgg tagaagaaac
    52441 cggcctaacc caaaatgtaa tttatccgaa tccaaacagt ggcagttttg tggtgaatat
    52501 gagcgatttt ggtaacaata tttacgaaat tgtaattttt gacgttctgg gcaagtgcat
    52561 ctataaagca aataccgaaa acggcaaaaa cgtttatatc agaaatcttg ctcccggaac
    52621 atattacctg aatgtaactt cggcttccga aataaaattc tccgaaaagc ttgtcatcat
    52681 cgaatgatag tgctaagcaa ttaggtgttt tacattataa atttttggtt caacataaac
    52741 aattggtatt gatttgtgtt gtacttttgc accaaaattt cctttatggc aaaagagatt
    52801 aaattcagcc ttgtctatcg cgacatgtgg caatcgagtg gtaagtacca gccccgtctg
    52861 gaccagttat taaaaatagc acccgttatt attgatatgg gttgtttcag cagaattgaa
    52921 accaacggag gtgccttcga acaggtaaat cttctgtatg gcgaaaaccc caatgatgtg
    52981 gttcgtcagt gggtaaaacc ctttaaagaa gcaggtattc agacgcacat gctcgaaaga
    53041 gccctgaacg gaattagaat gtatccggtt ccggccgatg tacgccagct gatgtataaa
    53101 gttaagaaag ctcagggcgt tgatattgcc cgttcttttt gcggactgaa cgatccgaga
    53161 aatctggagt tatcgataaa atatgccaag gaagcaggaa tgatatcgca ggcagcgctc
    53221 agtatcaccc attctgaact gcatactacc gatcattata tggctgtggt tgatgaagca
    53281 ataaaatatg gtgccgacga aatttgtttg aaagatatgg ctggaattgg cagacccaca
    53341 gaaatcggca gacttataag cgcgataaaa tcgtcatatc ccaatgtagt tgttcagtac
    53401 catggtcacg caggccccgg attttcagtg gcttcgatgc tcgaagctgc caaagcagga
    53461 gttgattaca tcgacacagc catggaacct cttgcctggg ggacatcgca tcctgatgta
    53521 attaccattc agtcgatgct gaaggatgcc ggtttcaaag tacctgagat caatatgaaa
    53581 gcatacatgc atgcacggtc gctgaatcag gaattcatgg atgagttttt gggtctcttt
    53641 ataaacccca aaaaccacct aatgtcgtcg ctgcttatcg gaagtggtct gcccggcggc
    53701 atgatgggaa gcatgatggc tgaccttgag ggcgttcatc aggggataaa ccagtcgctt
    53761 gctgctgcaa ataagcccca actctcgctc gacgatcttc tggtggcatt attcgaagaa
    53821 gttcagtacg tatggccaaa acttgggtat cctccactgg taactccatt ctcgcaatac
    53881 gtaaaaaatg tagctctcgt aaatgttatc aatttgttgc aggataaacc cagatggcag
    53941 ttgttcgaca agaatacctg ggatatgatt cttggaaaaa caggaaaact tcccggaccg
    54001 cttgattctg aaattatagc cattgctgaa gcacagaacc tgaaattcta cgatggaatc
    54061 cctcaggagg cctatcccga tgcccttgac caatacaggg aagaaatgaa gaaaaacggc
    54121 tgggaagtgg gcgaaaacga agaggaactc tttgagttgg caatgcacga caggcaatac
    54181 cgcgattata aatcagggat tgcaaaaaaa cgtttcgaag aagaactcga aaacgctgtc
    54241 gctaacagca aatcggcatc agccggtgaa cttattgcca taaaagctcc cgaaaaagga
    54301 aaaatactct ttggcgtatc gttcaataac ggcaaacgca atcctgataa aggagatatt
    54361 tttaaaaatg atgaaagaat ctgttatctc gagaccataa ccaactacga aaccatcagt
    54421 tgtccagcca atcttaagat taacaaaatt ttctttaaac acggtgacgt tgttcagaaa
    54481 gacgaaacta tccttttggg cgaaccgtta taaacacaaa aacacattaa taacaataag
    54541 ggggatgtgc atgcatcctt ttttttcggc tatgtaacat attttaaaag gtgcacccta
    54601 tagtgttaat tcatattttt gttcttcgaa tttaatcata acccatatgg caaagaaaaa
    54661 tatttcatca gttcacgaaa ggatttcaaa agaaatcgca tctcacagca atctcattgt
    54721 aaatgcatct cggaagactt ttatcaagga ttcggttgac aataaagaat gtgttgtttc
    54781 gaaatatggc acactggcaa catggacacc cattgattca acaggaagaa gtcctaagga
    54841 tacctatgca gtaaaacggg cttcaagcga aatgcatatc gactggactt cgcccaacaa
    54901 tcttcctatt gaagaagaca catttaatat gttgcttgac gatgccctta catttgcaaa
    54961 agaaagcgac aggctttatg tgactgacag ggttattggc gcggattcta aatatgcatt
    55021 accggtaaaa cttatcaccg ataaggcatt gcccggatta tttgccgata acatgttccg
    55081 ccctgttccc aaggatatca gcaaaagctg ttttgccaac aagccgtatt ttctgcttgc
    55141 actgccctac aggaaactgg atcatacaaa atataccgga agactacgta aacttgccga
    55201 tggaaccact tctgaccttg ttgttgctat ggatttcgac aggggtatcg gcattgtaat
    55261 cggcactgct tatctcggga gcattaagaa acttatgttc accgtgatga actattacct
    55321 accttttgaa ggagtattac cattgcattg ttctgcaaac gaaggcaagg atgggaaatc
    55381 ggctttaatt ctcggcttgt cgggaaccgg taaaactaca ctttcggcag atccatcgcg
    55441 cgctttgctt ggcgacgatg aacacggttg gagtgaagac ggcatcgcaa actttgagaa
    55501 tggatgctat gccaaaatga tcaatatcaa cccgaagaaa gaacctgata tttacaaggc
    55561 ggttctgcat caggccgatt atctgaagca tggggcaatc atcgaaaatg ccatgatgta
    55621 ccctaacggt gaattcgatt tcgatgacga acgccttacg cccaatagcc gtgcatcgta
    55681 tccgcttaca tcgataaaaa acattaaaaa gagctcgaca gccgggcacc caagcaccat
    55741 acttttttta actgccgatg catacggggt aatacctccc gtttcgaagc ttgataaatc
    55801 acaggcaatg ttgtggtttc tgatgggtta cacctcaaaa gttgcaggca ccgaacgtgg
    55861 tgttgtggaa ccctcagcta ctttctcccg gttctttggt caacctttta tgccgggatt
    55921 acccagcgtt tacactaaaa tgcttggaga aaaaatggag aaattcaaca cttcggtata
    55981 tcttatcaat accggatgga cagcaggtaa atttggtgtt ggccatcgta tcgacctcgt
    56041 acttaccaga aaaatggtga atgctgcact caacggagac ctcgaaaaag tagattacat
    56101 ctacgacaaa ctcttccacc tcagcattcc tgtttcatgt ccgggcgttc cttcagaaat
    56161 tctgaacccg ataaatacat ggaaagatcc tgaagacttc agaaaaactg caaatgaact
    56221 tgcccagaag ttttcgaaaa ccttcgacga ttcgtttggc aaaggccata tcgatcctct
    56281 aatcagaaaa aattgcccgg ggaaataagc aactgtctgt tatagtaaaa agccggggtt
    56341 tacaaccggc tttttttcgt agccatagtc ttcgaaaatt atctatattt gcatagaaac
    56401 gcgttgagca acaattaaat tttgttcatg aaaactacct tcaccaacag ggaaatcagc
    56461 tggctggctt ttaacaacag agtgcttcag gaagccgatt ccgaagaagt gcctttgtat
    56521 gaacgcatac gctttcttgg aattttttcg aataaccttg atgaattcta ccgcgtaaga
    56581 gtagcaacct taacccggct atataaacta aaagaaaagg atttcccgga gaaagcaatt
    56641 atttataaag acttgctcga tgagataaac gatatcaatg cccacaacca gaacgaagta
    56701 agtcgaatat tgcagaacat tcaagatcgg cttgaagaaa aaaacgtatt tctcatcagc
    56761 gaaaaagagc ttagtccaga acaaggctct ttcatcaggc agttttttaa aagttatgtc
    56821 cgccctaacc tgtttccagt tatcctgaaa aacatgcaac atccgagttt tttaaaggat
    56881 agctcaattt acctagcaat tatactgaaa aggcatggtt ttgccgaaaa aaccacctat
    56941 gcagtagttg aaattccggt aaaagaagtt gacaggttta ttgtattacc ccaagctgga
    57001 aacaagcatt acatcatcag actcgatgat gtgatcagat actgtctcaa cgacattttc
    57061 ggtgttttcg ggttcgacca gtttcaggct tttacgttca agctgacgag agacgcagaa
    57121 ctcgacattg atgaagacat atcaaagagt tttgtagaac ttatttcaaa tagtctgaaa
    57181 gcgcgtgaaa caggcaatcc cgtgaggttt atttacgata aggaaatgcc aagctccctg
    57241 ctgaatgcat tgatgaaagc actgaagata agaaaacgag atacagttat gcagggcggg
    57301 agatatcaca acaccaaaga ttttatgaac ttcccgcacg tatctgaaaa ggttctctca
    57361 tatgaaccca ttgtgccgct tccgaacaag ttcatacaac ccggaaaaaa aattacaagc
    57421 tctatccgtg aaagagacat tttactgcat tatccttacc agccctatca gtacataatc
    57481 gatttgctca gagaagcatc catcgacccc aaggtgaggg ctattaaaat gacattatac
    57541 cgacttgcaa acccatcgaa agttgtgaat gcgcttgtca acgcttctgc aaatggcaag
    57601 caggttactg tatttatgga atttcaggcg cgcttcgatg agtcgaacaa catatattac
    57661 tcgtcaaagc ttcaggaagc tggtgttcgc atcattaaaa ccattcccgg atttaaggtg
    57721 cactgcaaga tgattctcat aaaaagaatc gaaggagata aagaaaaact atatgcaaat
    57781 atcagtacgg ggaacttcaa tgaagctaca tccaggattt actccgacaa cacgctgctc
    57841 acctgtgacc caagaataac tagcgatgtt gaaaaagtat ttgagttgtt tgagagatcg
    57901 tacaaaccca tgcgattcca gcacctggtc atcagccctt tcagtacccg aaatttcttt
    57961 ttaagtttaa taaacaacga aattaaaaac gcaaaagccg gcaagccagc ctgggcaatt
    58021 atcaagctta acagccttgc tgatgaacaa ctttccagga aactcctgca ggcagccgaa
    58081 gctggggtgg ccttaagctt aataattcgt ggtatttgca ttttaaatcc ggaaggtcat
    58141 aaaaacatcg aaatcatcag cattgtcgat aagtttctcg aacattcacg tgtacttgtg
    58201 ttttgcaacg gcggcaaaaa taaatacttc ataagttctg ccgactggat gatccgcaat
    58261 ctcgacaaca ggattgaagt tacaacaccc atatacaacc ctgatattca gcaagaactg
    58321 ttggatatgt taaaaattca gctttccgat aatacaaaag caagaattat tgataatacc
    58381 ctgcgttcga aaatgaagat caacgatcag ccaaaagtca ggtctcagat agatatgtat
    58441 aattatttaa agaagaaaca cctttcatga tattcgctac aattgacatc ggttccaatg
    58501 ccggcaggct gcttatagct cacgtttacg aaaaattcag agtactgttc agcagtaaaa
    58561 ttgctctggt gagggttcct ttgcgcttag gtatggatgt ttttgaaaag ggctacattt
    58621 cggaagataa aatacaggat cttataaaag ctttgcaggc atataagctg attatggaaa
    58681 tatacaagcc cgttgctttt gatgcctgta caacgtctgc aatgcgtgag gccgctaacc
    58741 gcgaagacgt gctgcagcgt gttatcgatg caaccggaat aaacctgagg gttatcgacg
    58801 ggattgaaga ggcacaactt ataagtgaag cgaataacat ttatattaac cgcatttata
    58861 aagatacgct gtacattgac gtgggaggtg gaagcacgga atgctctctg ttcagcggtg
    58921 aaaggtttat tacttcaaaa tcatttaata taggtaccat taggctcatg ttcgacaagg
    58981 tgaaatcgtc ggaatgggat gatttgaagc agtggcttga atcgatgccg gtgcgggaaa
    59041 gtcagttgaa ttgcatttgc acaggaggga atatcagtgc cataacaaag cttttcggca
    59101 acaccgatca taccattgat tacccccaac ttattcatgc ctgcgaagaa ctcgagaagt
    59161 acagctatga ggaaagaatg gtgaaataca gtttcagacc tgaccgggcc gatgtgatag
    59221 tgcctgcctc aaaaatattt aacaatatca tgaaatgggg gtgtataaat cgcctcattg
    59281 cgccacaatt gggacttgct gacggccttg caatcgagct gtataaaaat cacctaaagt
    59341 cggggctcta aaacattcat catctggcga ataatctgtt gctgtttttc ggtgttttta
    59401 cccagcgaaa agctattacg aagtactgcc aaataggtat gcacagtttt tacattatct
    59461 gctggagtgt aaaactccgg tgcaggactc aattgttttt cttcgaggta cttatcgata
    59521 acggagcgcc cgaacaaagc cccctgctcc cacgggtaga tatagaaaag tacatcgtag
    59581 tgcttttcgg tagcaaaagt aagctcagca ataaacgcat ctttgtaggc gagcacaaac
    59641 aaatcaggca gcgcaataac aaacacaggc ataccaaggt tttgtgccaa agatgcatat
    59701 aacaggcact gcatcagagg gtcgcccgtt ttttgagata taacatggct caggcagagg
    59761 tgctcgcgtt taacaacatt ttcaaccggt ttgctgaggc aaaaaacatt gtaaagcacc
    59821 tgattcattg ttttgatttt ttcgatagca gtactgctgt tggtaagctc catccagata
    59881 ttgtgcctga ttttacggaa ggcatcaggc aactccactg tatcgtattc cgggaaaaga
    59941 gcgcgttcaa caatcagcaa ggcttcgaac aactgaggat tttcggctcc ggcccatttc
    60001 tgcaactgga agacatattt ctttgcctca atttcatcgt gtattagctg tattcgttcc
    60061 tgatacaaga tatcgctgct aagcacccag ctttgctcaa ggtatggttt cacttcgtcg
    60121 agatgctcga aaatgctgct gcggatagct tcgtacacct ttgggtcagg gtcttccagc
    60181 agtaaaaaaa gcgattttac gtccttttca ttcattgcaa gttcaattca ttttcttcga
    60241 ttcgaaccac ctgattaact ccttttattc tcagtatttt ttgtatcagt tgttccaggt
    60301 cagaagtgtc gtgaacaaac attttaatat ttccggtaaa cactccgtct tttgtaacaa
    60361 aatgcaattc cttcatattt acattggctt ctgaagaaat cacctgagta attttaaaca
    60421 ccattcctgt ttcatcgatg cctctaattt gaacgcgcac cgcaaacgac atcagctttt
    60481 ggctcttcca gtgtaccgat acaattttat cgccctgcgt agccatcagg ttaattgccg
    60541 tagcgcagtt tttgcggtgc acaatcaccg aattatttgg atttaggtaa cctactacat
    60601 catcgcccgg gatagggctg caacattggg caatagtgaa attttcctcg aacaactccg
    60661 ggtccacatc aattaccttt cctttcttta ttacatcgcc cgtctgattt ttaggtttac
    60721 tcaactgaag ccggaaatac tttttaaaaa tgctgggttt tttattacta agatgcttct
    60781 cgatatcatc gagcagaata ataccctgcc ctatgcgccg ataaaactcc tctttattgg
    60841 gaacatcata attatcgcga agtttcttga acaattcgtg gtcgagctgt atttcgagct
    60901 ccctgacttt ttgaataaac aattgctgcc ctttttcaat attaaatcgc cgggtttctt
    60961 tcagggcgtt cttaatttta ccttttgctt ttgatgtatt cacaaaagaa agccactcac
    61021 gttttggtaa ttgctgttgt gaggtgataa tctccacctg gtcgccgcta cgaagctcct
    61081 gagataaggg tctcagctga tggttaattt tagcggcaat agcagtattg ccgatatcgg
    61141 tatgaatttc ataggcaaaa tcgagggcgg ttgcacctac aggaacccgt accag
//
LOCUS       DAOOQP010000006        49987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_761_length_49987_cov_6.622026, whole genome shotgun sequence.
ACCESSION   DAOOQP010000006 DAOOQP010000000
VERSION     DAOOQP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 49987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_49987_cov_6.622026"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLK75_01270"
     CDS             <1..656
                     /locus_tag="PLK75_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOY37906.1"
                     /translation="KENTTIINGVGDKKLIEARVNQIKAQIESTTSDYDREKLQERLA
                     KLAGGVAVICVGAASEVEMKEKKDRYDDALHATRAAVEEGIVPGGGVAYIRAIEALSN
                     MKGENEDENTGIAIVKRALEEPLRQIVENAGIEGSIIVQKVKEGKDDYGFNARTEKYE
                     NLFKSGVIDPTKVTRVALVNAASIAGMLLTTECIIAEIPEEHPAPQPGMGGGMPGMM"
     gene            complement(767..2335)
                     /locus_tag="PLK75_01275"
     CDS             complement(767..2335)
                     /locus_tag="PLK75_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37907.1"
                     /translation="MRKTLFFFVAIAMPLITISQERFYNLYEGLVIKQMFIINGHYIS
                     FGVENLSETVILMPSFNIIEASGELTESFYYLDTTDGYTFNGNRPCILIEDTLFIAGA
                     AVDYYKAYTLEPRIMKYNLGMRTMDTIISYNHCVQTTNPQKATLDLIDTTSLGFVITG
                     SYVYNSQNSFPLVIFTNRTGDTLGVKQYPFLPANPYRRRVFPYQTIAVPAGGYLMSCQ
                     DEIGDVVNWPDRQRAWFVRLDNEGNELWRRTTATQDTICYHPFAFVMPDGNYLITWSD
                     PYLYHAINEENSSLWLARMDDNGNISQKQKIQDYISDLPNRYFWVNDYYQDSLGNIYL
                     TGEAVWSRYFGFILKINPQGEVEWYRDYECFPENSVNTPWTKFYSITPTHDGGFIMGG
                     EYYSPPSSMFPAGVQKGLAVKVDACGCLEEGCNPDCHNGYATQTIRAMQAAVYPNPAS
                     DVVTVSLPVKGSSARIELFDATGCQWVDVARECSETTTGSQVKLNVAGLPAGVYVLNV
                     WVGGKLCTGKVVRE"
     gene            complement(2344..6549)
                     /locus_tag="PLK75_01280"
     CDS             complement(2344..6549)
                     /locus_tag="PLK75_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37908.1"
                     /translation="MKKFLLIIVFVTTIVSGLQAQTINFNLLVDKDTTINVNTKFLGK
                     CSAASVTATVTLHSDTSLVRIIMVDALGKRRLLLEAYPLICSDSVVHFTAYYDETKYM
                     PYNTNPDILEFYIEDAECNFSSMAFSNKIVLNYIVLAQQYKQTIESQKVDQINENLEI
                     KGLPWRARVTKMNTADYSKKAQYWGGDKYFIDGYDYYAGGIFCEYSQWGEIINENIGE
                     KSTIDTLPPVKAKSFDWRKAHGANLNTSPYFDENPDYGIINIENTAYLVNENNGWLTS
                     VKDMHTWGSCPNGCYLFAPIGSLEAVINLYFNQHLDYNISEQHLADCFNVYSPTSDCI
                     NGGKTDSLDNFINTQNYLFVDESNFGWNDSMTGYCCDISTATFKIDINEMITIGDSEN
                     EVKRALVIYGPLSAKPKSNHIATLVGYKQIQAFDTLNINYGWRVEVDTINHLFIPDTL
                     VIYPGNKLIGRTMWIFKESASLDRGVMGYFNVLGKLNSMPTSNDAFAFETPIADLMNE
                     TLEPLYFDGDGDGYYNWGIGEKPAGCPGPDLRDCNDNNPNQHHYNAYLQCVCACDSLE
                     AWKSDVPIYITSNTILKDTIITRPIIVQGSNVMLTLQGDIQCTENVYIQSEIGTTIEV
                     ENANIFNGCDTLWQGFRITANLDPTLGITGRSLVMIKQSYIEDAKYGICSDAGAIIRA
                     NNSCFKYNFCDVLIVPLQMSDYYDQVLGNNTSVFENCHFSTDNEQHYEELAPNVILVN
                     TKGVQFKNCRFIDDGVDNGLFNEDNRMGIWTYKSGFRVDEACYFGNLGSAIRAYNGSY
                     LPIDIEDNLFETCHYGVYLSQSHGARVVNNIMRFVDCDSTDAEVLPYCVYLDNSYDFQ
                     LEGNTLYVFQAGASDVINGVIVNDVYSNQEKIYRNNIQHMRVSIEAIGLNRSSSAPTG
                     LQIRCNNFQYNDEDVFVTNNLDNPYPPGYPVGIAENQGSMLNPAGNKFTHWGTGNAYI
                     NMINTSDHNINYFYHSTSTNIRFYPDSIVGYVNRVPTGVYFTDSTCLDNTDTTGNDTI
                     IILPELRMLSGGIDEIDNTLEALTDGGNTPLTIAEIVLASDLNAWQTYLGLMDKSPYL
                     SNEALKEVALKEDALTKPMVRDVLVANPQASKDPEIQKILDERIEALPQYMIDQINSG
                     LTQISPKEYLEMQRAELSAELSEKTSRQLRYYLRHPELYNDGDVEELLSISQEPQYGM
                     KLAAYYGGKGDYGKAIEALNAIESNNVILMTEAENLAGLYELLEEIESDSVALNRLGV
                     ERINALLDIEASGGQAGAYARGLLMLNGASDYKEPVYLPDVMANRKLKTNHRLPEKEF
                     MKVYPNPADKYITVEYSIATEGINVVLKVVNTESKTFFAKKLESPEDIILIDIRNFSQ
                     GNYIVFIEVQGKIVDSKVVSVTK"
     gene            complement(6662..8608)
                     /locus_tag="PLK75_01285"
     CDS             complement(6662..8608)
                     /locus_tag="PLK75_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOY37909.1"
                     /translation="MIAINNISLMFGNFELFSNIGFMVNGRDRIGLVGKNGAGKTTLL
                     KIMNGQIQPTSGSVSIPSGIRIGYLPQHLNVSDSGRSVFEEAEEAFTEIKELEREIKT
                     LQEALSSRNDYHSSEYEKLIQSLSLANDKYHLLKQDNTEASVVKTLKGLGFTDAELNR
                     KTAEFSGGWRMRIELAKILLLQPDVLLLDEPTNHLDIIAIQWLEEFLKQYPGAVLIIS
                     HDRTFLDNITTRTIELSLGKLYDYKVPYSQFVALREERLKQQKAAYNNQMKMITETQE
                     FIDRFRYKATKANQVQSRIKQLEKIELVNFDEIDTKSIHFRFPQSPRSGSIVFETISL
                     GKEYSGHIVLNEIDFSMERGDKIALVGRNGEGKSTFSKIVAGELDYSGTLKKGNNLHI
                     GYYAQNQEEMLDGELTVLETLDYVAIGDIRSRLREILGSFLFRGDDIDKKVKVLSGGE
                     KSRLALARLILEPYNLIILDEPTNHLDMQSKDILRDALMKYDGSLLIVSHDRYFLNGL
                     VNKVYEVMGTKIKEETGGINAYIKRYRKQIQMQLFSYSKPPTKNKEENTEQKQIYIER
                     KELEKTIRKIRNEHHNHEKQIELLEQQINDIEIFINSPDKYKEPRGDVYLDYEKLKKR
                     LESEMHLWEKTGVELIRFESIKQQ"
     gene            8710..10092
                     /gene="fumC"
                     /locus_tag="PLK75_01290"
     CDS             8710..10092
                     /gene="fumC"
                     /locus_tag="PLK75_01290"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOY37910.1"
                     /translation="MTHRIEHDSLGNIEVPSDCLWGAQTQRSLNNFKIGSQLMPFQVI
                     ESLAIIKKASAIANNSMNLLSEEKKNLIIQVCDEIIAGKHMAEFPLSVWQTGSGTQTN
                     MNLNEVISNRAQELSEISSTEKGKKLHPNDDVNMSQSSNDVFPAAMHIAAYTVLVNTT
                     LPGLKQLAASLKKISNQHKRAVKVGRTHWMDATPITVGQEFSGYVALIESAAGRIKNA
                     ASRLLFLPLGATAVGTGLNAPKGFDAKVCSEIATLTGFPFKPSRNKFEALSAHDALVE
                     VHAALKQAAVSITKIANDIRYMASGPRCGLNELLLPENEPGSSIMPGKVNPTQVEAIG
                     MVCCQIMGNDTVVTVAAMQGSFELNVYKPVIIRNTLESAVLLGDSCRSFAMHCVDGIR
                     VNNDKLKYYLSQTLMLNTALSKVIGYEKAAQIAQIAMRENLGLKDAAIKSGYVSEADY
                     NRFVDVNKMI"
     gene            10136..12001
                     /gene="asnB"
                     /locus_tag="PLK75_01295"
     CDS             10136..12001
                     /gene="asnB"
                     /locus_tag="PLK75_01295"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY37911.1"
                     /translation="MCGIAGFYTNADTFASRDELDKMVAALVHRGPDASGVYFSNTVG
                     LGHRRLSILDLSQDANQPMESHSMRYVCVYNGEVYNFRSIAKDLNIDFKTTTDTEVIL
                     EAFAEWGVTFINKLNGMFALAIFDKQEHKLYLFRDRIGVKPIYYYWDGRNFAFASELK
                     SLLQIKSVRDSRQIDFQSISHYLHLGYIPEPGSIYKNIYKFPSGNFGILSETGLRIES
                     YWLIEGAVRRQLIEDEVECKQRLKDLIESSVSMRLISDVPFGTFLSGGVDSSLVTAVA
                     SKYSAKKLNTFSVGFYDSKYNESTYARRVAEYLGTNHHEMVVTELDAQHMVLDLLNYY
                     DEPFADSSAIPTMLISKFARENVTMVLSGDGGDELFHGYGAYRWARRLNYMPTRLARQ
                     LIGFVLANSGNNKYQRAAHLFRYQERKHLKSHIFSQEQYLFSLAEINKMLLHDIKIET
                     TVDQDYSYFVRKLTPAEDQAIFDLKYYLKDDLLVKVDRASMKYSLETRVPLLDYRIVE
                     LALNISPKLKIQGSVQKYLLKEVLYEYLPREYFDRPKWGFSIPLSKWLSKDLSYLIDE
                     HLNEWMIRKHKLVNFEETNKLIKSFRSGTDYLYNRIWQLIILHRFMEKDKNIPIL"
     gene            complement(12018..13454)
                     /locus_tag="PLK75_01300"
     CDS             complement(12018..13454)
                     /locus_tag="PLK75_01300"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37912.1"
                     /translation="MKRGYKDILWFVLLLILSSSLIIDGSGLFKFNFKVLEGFTSKHS
                     LPAFSAKTWIDESFQDSLSTYIDNNFKLRPPMLRAYNEYKYRVFRKTNAAEVVLGKNN
                     QCLQTGYILEHTGDYFVGNNLIEERCKRIKYLQDTFQQIGITMFVVFAPGKASFMPEN
                     IPDRYLRSKYDENNYTCFVKNCDKYNIRTLDLQQYFDEIKDTVSYPLFPTNGIHWSEY
                     GMYLALDTFLNYTAKISSKKIPELTISGLKKTNKPQGEDYDVGKIMNLMLPLPEKTLA
                     YPQFSKSDTTRPELDILIVGDSFMFHWLKYKLGEYLFRHYNLWYYNVMVYPEFYQKEL
                     YNYSVNIKEELLKRDVIVLECTETFMHTAFWNFEDMAFKLFNPDYTPDAVFYYQNDIT
                     KDRKQFISIFEKAKKSKRRLNDVLYEEAVYRAKTMPLGSVEYYVYSIKNSEQWLDLMK
                     KQAREQGKHLDEIIMANAKWMTENDDYE"
     gene            complement(13454..14875)
                     /locus_tag="PLK75_01305"
     CDS             complement(13454..14875)
                     /locus_tag="PLK75_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOY37913.1"
                     /translation="MVFSSSIFLLYFFPIFLLLYYLSDRKYKNLVALVASLIFYSWGA
                     PLFIFVVLGSIFIDFYLIKAMHASGGKKRHWLITASVVLNIGLLVYFKYMNFFVENVN
                     VILGALGANSLHWTKIALPIGISFFSFQKFTYGIDVYRGKHKPLDSVWDLCLYILLFP
                     QLIAGPIVRYSEIADQLHNRHDYETVDNRLLGFYRFVIGLAKKVLIANVMGAEADRIF
                     AMQPIDMSTGTAWIGILAYTFQIYFDFSGYSDMAIGIGRMIGFRFPENFNNPYISQNI
                     TEFWRRWHITLGRWMRDYLYIPLGGNRVNNKWRLYFNLWVVFLISGFWHGASWTFIAW
                     GAFHGLFLILDRLFLLKLSAKIGKYPSIIFTFLVAIIGWVLFRSETLGFAFAYIGRLF
                     AFDFSTFEYFDREFWVMLAVALIFSFLTAFRFGFNLEKKILYSEYTKSGYFIMAIVAM
                     VLLFVCTASISASGFNPFIYFRF"
     gene            complement(15096..15695)
                     /locus_tag="PLK75_01310"
     CDS             complement(15096..15695)
                     /locus_tag="PLK75_01310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOY37914.1"
                     /translation="MLLRCMHHDLIEAGCDEAGRGCLSGPVFAAAVVLPQNFRNEMLN
                     DSKKLNHKQRSILRQIIENEALAFAVANVSNQRIDEINILNASIECMHKALQKLTIQP
                     EHILIDGNRFKKYGNIPYTCIVKGDGIYMPIAAASVLAKTYRDDFMEKLHTDFPQYNW
                     KQNKGYPTIKHRRAIEQFGPTTWHRMSFKLTDTQLKIVF"
     gene            complement(15697..16818)
                     /locus_tag="PLK75_01315"
     CDS             complement(15697..16818)
                     /locus_tag="PLK75_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOY37915.1"
                     /translation="MNIAVNMRLVIQDKMEGIGWFAYETMSRLAKNHPEHNFIFIFDR
                     PVHPSLLFPKNVRIIIKGPPTRHPLLWYMWFEWIIPAILRNTRADLFISPDGYASLKT
                     KVPTICVIHDINFIYHPEYLPSLAARYFTYFIPKFAKKASRIGTVSEYSRMDISKSFG
                     VSEERIDVYYNGANDAYRPMNPVLQQEIRNKYTRGKPYFIFVGALSPRKNVPGLLKSF
                     DRYCEMGGESSLVIVGDALHKTEEITECYNGMKFAKRVHFVGRLKVDELSKVMSSAIS
                     LVFIPFFEGFGIPLVEAMYCDTPVLCSNVTSLPEVVEDAALMSDPNDYESIASNMLLI
                     ETDSILRNKLIENGRLQRAKFSWELSAERVWMSIEKLTR"
     gene            complement(16818..17567)
                     /gene="kdsB"
                     /locus_tag="PLK75_01320"
     CDS             complement(16818..17567)
                     /gene="kdsB"
                     /locus_tag="PLK75_01320"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOY37916.1"
                     /translation="MKFIAIIPARYGSKRFPGKPLALINGKIMVQRVYEQALKAFEFV
                     YVATEDTRIFDAVNSFGGNVVMTSKKHRSGTDRCAEAIRIISDTLKTSFDVVVNIQGD
                     EPFIQIEQLNEIKKCFRSKSTQIATLIKPINNQDDIFNPNKPKVVINQKNEAIYFSRS
                     PIPFIRGVKELRWITNHQYYKHIGLYAYRSGVLTEITKLLPSPLELAESLEQLRWIEY
                     GYKIKTAFTEHESISVDTPKDLEQIRQIGLI"
     gene            17651..18739
                     /locus_tag="PLK75_01325"
     CDS             17651..18739
                     /locus_tag="PLK75_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOY37917.1"
                     /translation="MTNDSHNKMQRQMSPTRIILPMLIGLAVVAFFIIRDFKPGMFSD
                     VSFNTGVGTMLLLACVMMFFRDFGYVLRIRYLSDKKLSWKQSINIIILWEFSSAVTPS
                     AVGGTSVATYFLWKEGFSAGKSASIVMATSFLDELYFTLMFPILFLIFSHTGLFPLDE
                     NGNLNSSFLVFAGVGYGIKLLWTLVMATALFVKPVAMKNILNTLFKLRILRKWKEAVA
                     HAGEDIVAASHELRHKTIGFWIRSFLATLFSWTSRYWVLNFLILALAFGLPYFDAHTL
                     FSVGEHFLIFARQLVMWIMMLVMPTPGGSGFVEAVFTSYMADFIPIVGFVSAMALLWR
                     FVTYYPYLILGAIVAPRWFAKSRTKIGN"
     gene            18770..19210
                     /gene="rpiB"
                     /locus_tag="PLK75_01330"
     CDS             18770..19210
                     /gene="rpiB"
                     /locus_tag="PLK75_01330"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOY37918.1"
                     /translation="MEKTKIGLACDHAGFAVKDFLHGYLEGNNYEVIDYGCFSEDSCD
                     YPDFAHKLALGFENGECVAGIAICGSGNGINMALNKHRYIRAALCWNEEIAKLARLHN
                     DANICAIPGRFVDAMLAANIVDVFLSTPFEGGRHQRRIDKIPVH"
     gene            19254..19790
                     /locus_tag="PLK75_01335"
     CDS             19254..19790
                     /locus_tag="PLK75_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37919.1"
                     /translation="MKEVNQTGCCPPFDPKPWNNNTHHWNNKRFVKDHVITEHWVPVN
                     FGEVVQRIMEKMDAAGAQSPGWMGLSDHTSEKNMDIYVESDREVPGIENVSISATIFS
                     KVYDGSFDKTQLWCDDFNKEAQKRGFQIKKSYMWYTTCPKCAEAYGNNVMQVGVCRPV
                     LRLRQGYYAYLRLWAGYF"
     gene            complement(19891..20565)
                     /locus_tag="PLK75_01340"
     CDS             complement(19891..20565)
                     /locus_tag="PLK75_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37920.1"
                     /translation="MKKIVLFLALCQISIFVFSQNFKIGAYFCPSNQVASYLKGSEFL
                     KSDRKMFQSWEYGLFFDYKFNETYYFKASIGYSQNHLSLPNSLISSFQNNDIPLGIIL
                     KANVLKIKNLSIYIENGLSIAFCNSYHYNGVIGASTSSLTGTISDYNFKNDWSFGFIN
                     GVEISYLVKSKFEINVIVNYYSGINKVWENNRISINEDGVMNNYTISSNGSSFNVGVG
                     LGYCFY"
     gene            complement(20578..22161)
                     /locus_tag="PLK75_01345"
     CDS             complement(20578..22161)
                     /locus_tag="PLK75_01345"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY37921.1"
                     /translation="MKKSKGLLLKMFLFSLIISVVSCQKEDSSRETSNLRNTSAYDIE
                     SLMKEEIVIEMLNVLKPYSFKVNNKGIFEFDKISDLDSVLNILSRYANKLDDPKDIYP
                     NDPVLFAFEYHYGFKSLRNAIDQEVIYLENLDMLTEFNDPDNDKIVGDDFRSILTPNR
                     ELIVEDIICVYLDSITVGIHNYNYDALRDVHGAYLKSGVSGIAELCLQRDIVTLPADM
                     DLRTMVIDFYYVNINNNSLSYRFTPIVNYTNENPNHFSYYWQFDDGQSSTEREPTHTF
                     AASGEFSVKLTVTYGGSGLSTEKDILVGSCNANFTSLSDQDIVGKYYFTNTSTASIGN
                     IVSYDWYFGDNTPHVTTQSPSHAYSTDGTYIVKLVISTDAGCTDSFESTLHVSGSGDC
                     CVMYDKEKDTTRTYASGTRKAKLVIKIVNVWPVHGFASKTINYRVKNNGSLALEKADK
                     ITTTIAGFVYATNCTDYAAVDQGEVKYNKNKATYTYYQTHTPLRVRKNGLFSGYYVKD
                     NDGTSTGNNALSLHQKNCN"
     gene            complement(22366..23265)
                     /locus_tag="PLK75_01350"
     CDS             complement(22366..23265)
                     /locus_tag="PLK75_01350"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY37922.1"
                     /translation="MNIFVEIKDSFTKGTILTKLIYTNLLVFFLLISTLAVLYLPNLN
                     LSEFKSFFELPPSLLGFITHPWTLITYMFTHFSFLHILMNMLFLYWFGKVFLQYLTER
                     QMLGLYLLGGLAGGLAFVLSYNTIPVLGNNPAITPATGASASVMAIIFAIVSLVPKHD
                     VYVPILGKIKLLYIGLTAVAIDIISIPLQNTGGHIGHLGGAFIGIIFSLQYRKGKDIV
                     SWLLKITDGIIIIFSKKKKIRVTSRNKNKASYSKYDDMEYNARKKADQDEINAILDKV
                     ARSGYGSLSASEKEKLFKMSSKK"
     gene            complement(23265..24011)
                     /locus_tag="PLK75_01355"
     CDS             complement(23265..24011)
                     /locus_tag="PLK75_01355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY37923.1"
                     /translation="MNQYSPGGFFSLPPVVKNILIINIILFLATYILQRTGINLNDYL
                     AIYSIKSSHFQPYQLITHMFMHGSIAHIFFNMFALWMFGRVLEDIWGPKRFFIYYFVT
                     GLGAAFLHLLVNYIEITMLINKMDPVAVDVVLSQGADLLQGGQNYADTLMGKLNLMIN
                     IPTVGASGAIFGLLLAFGVMFPNAIIYIFFALPLKAKYFVMIYGGLELYLGFANRAGD
                     NVAHFAHLGGMLFGLIFLLIWKKHRFNRWN"
     gene            complement(24021..25841)
                     /gene="mutL"
                     /locus_tag="PLK75_01360"
     CDS             complement(24021..25841)
                     /gene="mutL"
                     /locus_tag="PLK75_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOY37924.1"
                     /translation="MPDIIRLLPDAVANQIAAGEVIQRPASVVKELMENALDAGATDI
                     QVIIKDSGKTLIQVIDNGCGMSAPDARMCFERHATSKIRNANDLFSIRTMGFRGEAMA
                     SVAAVAEVELKTKRQEDETGSELHISASKLMSQELCNCSPGTSIAVRNLFFNVPARRK
                     FLKSDQAEFRHIVEEFQRIAIPCCDIRLKLVHNGTDILQLEPSNLRQRIVSVFGKAIN
                     QHLIPINIETSIVSVEGFIGKPDKTRKTPDEQYFFVNKRFMLHPYFRKAVMLPYEGII
                     MQSTYPSFFIFLQVDPSHIDINIHPTKTEIKFDEEKAVFQILNASVKESLGKFNIVAP
                     LAFEEDITKDVHLTSRTEFRLPEIKLNPDYNPFDKPNRQKEYYDSFNKNFAETRKPPA
                     NWEMLFDTEKKQPQGEPEKGIESVSVSEDVQFLQIKDKYIITPVKSGLMIIDQGRAHE
                     RILYEEFLSHMVDKKATCQKSLFPEQIELSATDRALLTEVMEDLCNIGFDISFLGKST
                     IAIHGVPSALTDINPCLIFKEFFQTFNDMGQQLKLSYNEKLAYSIAKTTSLHSPKQLS
                     SLEQKDLFFRLMLCKNHNFTPDGKKIMEIVAIDEIEKKLN"
     gene            complement(25844..26155)
                     /locus_tag="PLK75_01365"
     CDS             complement(25844..26155)
                     /locus_tag="PLK75_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37925.1"
                     /translation="MAFSFFKLPGHSVFEYQPRYFDLEKERINKRRRQLKLERGEDIT
                     DPDNPGSSIKGSYSSLFQRNSKYKKQSAVRILIRLAFLAVLIYLLMQVDFSSFLNFIN
                     N"
     gene            26382..27797
                     /locus_tag="PLK75_01370"
     CDS             26382..27797
                     /locus_tag="PLK75_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37926.1"
                     /translation="MKALGVTLIIFILLNSYLFAQHTTELYKVFGSPYSSTINCICTD
                     MDGSTYVGGEFTKSLLMGPYKFKDKWGGFIAKVDDQNQVIWMKRADMKFRNMELIDSS
                     LIVFAQNSQTVNFAGTELQYRGQNFNSVLASISINTGDVKWIKEISGEGDVYAADMTI
                     CKKDIFITGSFRVGMALDKYTLKQTHEKNNFIARLTAEGKVIWLNKVSGGDSFITGAT
                     ISAIAANQNGDFVISGEIAGSADFAGYCFDVAEFNSKSENTTSEGCVYYAYFTGDGKC
                     IRVNKAITEAEVTDIALFGDQVYLCGYYSGAYLLQRPIAASIFGDTVVLNSVQSPTGS
                     LLETWFVISLRDNEPLWALTTKGKNSSRALSLVVDDQGSCYAGGYFYDHIILPDGSEV
                     NAINAEFSAGALVMKIDRSGKLLWTHTASSPTGINKFFDVSLNHSLVVCGEISGSAVF
                     DKSLIQSRGKHQNGFTYKFDL"
     gene            27810..29642
                     /locus_tag="PLK75_01375"
     CDS             27810..29642
                     /locus_tag="PLK75_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOY37927.1"
                     /translation="MNSLNDFIHILEAENQLIRVQSPISPNLQITEIADRIMKSPDGG
                     KAILFENTGTAFKVLINAFGSEKRMGMALGYENADQAAFALASVLQILTREKESFWNK
                     LKMVPVLTKIASAIPTLIKKRGKCQEVIQTNPDLDILPILKCWPFDGGKFITLPIVIT
                     KHPETGVRNSGMYRMQVLSKNTTGMHWHLHKGGAAHYEVYKKMKMRMPVAVALGGDPV
                     YTYAAVAPAPDDIDEFMLAGLLRKRKVKFVSCITQNIEVPADSDIVIEGYIDTEEDFC
                     NEGPFGDHTGMYSLPDAYPVFHVTAITHRKDAIYPATIVGVPPMEDRYFALATEKLFF
                     PALNFLYKEIVGFHLPWFGVAHNLVLVKIRKRYPGHAFKIMHGLLGNGQMMFSKTIIC
                     VDEDLDFNKPETWLNRILQNIQPAKDVETGSGPTDVLDHAAYRFAYSGKLLIDASNLV
                     ESDMENPVHTKNPDLPNYSVVSLGTCLLAFPQANTKPDLADFAERLSHQTASGEYKVV
                     VITEKAIIDLSPELKAWYILNNFDPGHDSLVVNNCLFVNATIKTTAKDGFSRNWPNIV
                     SMDDKTIADIDALWPLIFPDSPMISPSLQYKKLIVNKGASITSK"
     gene            29647..30303
                     /locus_tag="PLK75_01380"
     CDS             29647..30303
                     /locus_tag="PLK75_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37928.1"
                     /translation="MSNSYESVLNHIELHGYEFRFVEYFKNGFRLFKKYPFGFLSYGI
                     NISLVIYFSGFLGIPGSIISNLLQPVFLAGIYIVANDLLREQTPGYTRFFSGFEQFAK
                     LFVAQIVTFSLVFLGLLLLVLPGIYLAVCYSFTVFFIVFMKYDYRKAMDWSRKIVRIN
                     FWPVLGFMLVIGLINVLGALAFGIGLAFTLPFTACVMFSAFEDVISTAAEKTGGVGEY
                     "
     gene            complement(30310..30834)
                     /locus_tag="PLK75_01385"
     CDS             complement(30310..30834)
                     /locus_tag="PLK75_01385"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOY37929.1"
                     /translation="MTLNNNDTRIYLIGFMGCGKTTIGKKLAKRLHYTFVDLDEEIEK
                     TVKTTIPDIFKNQGEEEFRRLESILLKQFAACKNFVMATGGGTPCYGENIDVMNGNGI
                     SIYIKLPAISLIQRIKKSKKNRPLIFGKNDDELEFFVKQALLKREAFYCKATYVVEGL
                     RTDPEKIAELLSIR"
     gene            complement(30821..33592)
                     /locus_tag="PLK75_01390"
     CDS             complement(30821..33592)
                     /locus_tag="PLK75_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOY37930.1"
                     /translation="MSEDQEISGFDDVEETSENGIEEHVADIQYTRLTGMYQDWFLDY
                     ASYVILERAVPHVYDGLKPVQRRILHAMKDLDDGRYNKVANIIGHTMKYHPHGDASIG
                     DALVQLGQKDLLVDTQGNWGNVLTGDAAAAPRYIEARLSKFALEVVFNPKTTFWKNSY
                     DGRNKEPITLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDACIATLKNESFELYP
                     DFLTGGMIDVSKYNDGIRGGKVRVRAHIEKLDKKTLVITDIPFGETTNSVIDSILLAN
                     EKEKIKIKKIDDNTSSNVEIVIQLPPAVSSDKTIDALYAFTKCEVSISPNSCVIEEDK
                     PRFMGVSDILQASVYNTVKLLEQELLIRMRELEEQWHMLSLEKWFIEKRIYKDKAYEN
                     SQTNDEAIEFIGTKARASQQLFRREITNDDLLRLLEIKMKRIIRYNAEKAEEEMTSIE
                     GQIEEVKHNLEHLTVYAINYYTHIKAKYGKGRERKTEIRNFDIINTSNVAVANEKLYV
                     NRKEGFVGTGMKKEEFVCECSDIDDIIVFKRDGKYIVTKVSEKAFFGKDIIHLDVFRK
                     NDTRTIYNVVYRDGRFGNYFIKRFAVTGVNRDKEYDITKGELGSKLVYFTSNPNGEAE
                     IIKIYLKPKPKIRKLIFEADFKEIAIKGRNSLGNIITKNDIHKIVLKEEGVSTLGGRK
                     IWFDDEVLRLNADGRGIYLGEFHGNDKIIVVYKKGTYCLTNFDLSNHYDEAILLIEKY
                     NPDKIWTALYYDAPQGFYYIKRFLFEDTTKDQFYIPEDEGTKLIMLSDDRFPQVEIIF
                     GGKQASRPKEIIDVDNFIAVKGIKARGKRISNYEVEKTDFIEPLQKEIPAENLDSDIE
                     PDEFDQDETPDEIEFEIIDTTSIEEETVTRDLPKPKKAKPSTKPAKSDSSKDKEPESD
                     PKQMSLDFE"
     gene            complement(33629..35464)
                     /locus_tag="PLK75_01395"
     CDS             complement(33629..35464)
                     /locus_tag="PLK75_01395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOY37931.1"
                     /translation="MVANYSEETIKTLDWREHIRKRPGMYIGKLGDGSSRDDGIYVLL
                     KEIIDNSVDEYAMGFGSKIDIEVEESTVRVRDYGRGIPLGKVIDVVSKMNTGAKYDSK
                     VFKKAVGLNGVGIKAVNALSSDFRVVAYREGEKKEAVYSRGILINDAPIEKTDEPAGT
                     LIVFSPDIELFPSFRFRTETIENMLKNYVFLNTGLSIYLNGQRFFSRNGLVDLLNDNM
                     NSECLYPIVCLKGEDIELAFTHSNKYSEEYFSFVNGQHTTQGGTHLSAFREAYAKTIK
                     EFFRKDFDSSDIRQSIVAAISIKVEDPIFESQTKTKLGSRDMAPDGISINKFVNDFIK
                     KELDNYLHKNPETADAILRKIIASEKERKAIQDIKKISRERSKKVNLHNKKLRDCRVH
                     YNTDEERRFESTIFITEGDSASGSITKSRDVNTQAVFSLKGKPLNTFGLSKKIVYENE
                     EFNLLQAALNIEDGIENLRYRNVVIATDADVDGMHIRLLLITFFLQFFPEVIRNGHLF
                     ILQTPLFRVRNKQETLYCYDESEKQRAIAKLKGNPEITRFKGLGEISPDEFKNFINNN
                     IRLDPVNLSKADMINDLLEFYMGKNTNQRQLFIIENLRIEEDAIV"
     gene            35646..36581
                     /gene="rsgA"
                     /locus_tag="PLK75_01400"
     CDS             35646..36581
                     /gene="rsgA"
                     /locus_tag="PLK75_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOY37932.1"
                     /translation="MLQGLVIKSFGKTHYVKTSLGILKCSLKGALRIKGYRSSNLVAV
                     GDRVSVEQENQGEGSIAEIFDRKNYIIRRSTNLSREVQIIAANIDLAILMVTLKEPTT
                     STVFIDRFLVSAEAYQIPVLLVFNKIDSCKGNDIEKLAEMMAAYDMAGYQLVSVSVTK
                     RINIDKIREAVEGKTSVLCGLSGVGKSSLINVLDPSFCLPVSDISETHLSGKHTTSFA
                     QMLELNFGAYIIDTPGIRGFGIVDIKKDELYHFFPEIFKFSKSCQFYNCLHLNEPGCA
                     VLSAVADGNIAESRYRSYVSMYEDENKKFRKNKFL"
     gene            36690..37805
                     /gene="lpxK"
                     /locus_tag="PLK75_01405"
     CDS             36690..37805
                     /gene="lpxK"
                     /locus_tag="PLK75_01405"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOY37933.1"
                     /translation="MKQPKNIVVLIMLWPFAVLYRFITALRNFMYDNNILPSVEFDIP
                     VISIGNISVGGTGKTPHTEFIIGMLREKYRIAVLSRGYKRKTSGYVEVSETSSYLQVG
                     DEPLQMKRKYPETVVAVCEKRVKGIKILMEKYPDLNLIVLDDAFQHRRVRPGLSVLLN
                     NFYHPLSSDHLLPYGRLRESPSGVLRAHVIIVTKCPENMKAIERRIMLKEMDPQPYQY
                     LFFTRFGYHPPRTVFSTPIELDITDLKKYHVLAVSGIAFAKDFIDYLNSTFASVDHVE
                     FADHHDFRKRDIEKIEARFNAIAGYKLIVTTEKDSIRLLECGFMPDALKRYFFYIPIS
                     VILQGSEEDKKQFDNQFFTYVSNNKRYSSLYKGTNLR"
     gene            37750..38559
                     /locus_tag="PLK75_01410"
     CDS             37750..38559
                     /locus_tag="PLK75_01410"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOY37934.1"
                     /translation="MFQTIKDTAAFIKAQISGNPEVGIVLGTGLGGLVSEIEIEKIIS
                     YNEIPNFPVSTVEGHSGQLIFGKLSGKFVIAMKGRFHYYEGYALSQVVLPIRVMAQLG
                     IKNLFLSNASGGVNPDFEIGDLMVINDHINLIPNPLIGKHEPEFGVRFPDMGQAYNPT
                     LIHRAGEVAKALGIKLMYGVYTATTGPTYETPAEYRYFRIIGSDAVGMSTVPEVIVAN
                     QMGIPCFAMSIITDLGVPGKIVTITHEDVQKVAAGAEKKMTQIFKKLIADL"
     gene            complement(38637..41213)
                     /locus_tag="PLK75_01415"
     CDS             complement(38637..41213)
                     /locus_tag="PLK75_01415"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOY37935.1"
                     /translation="MLLVEPQTETKTVTENLRTYTDEEAFLATLDYFKGDDLAARVWI
                     NKYALKDSDGNLYELTPDDMHRRLARELHRIEMKYKNPLGEEEIYQLLKDFKYVVPQG
                     GPMSGIGNNMQIVSLSNCFVIGNDKDADSYGAILKVDQEQVQLMKRRGGVGHDLSHLR
                     PKGTPVKNSALTSTGIVPFMERYSNTTREVAQDGRRGALMLSISVKHPDAEDFIDAKM
                     TPGKVTGANVSVKIHDDFMKAVVDDKPYIQQFPIDKPNPKFQKEINAKKLWDKIIANA
                     WKSAEPGILFWDTITRESVADCYGDKGYRTVSTNPCGEIPLCPYDSCRLVAINLYSYV
                     ENVFSTEARFNFDKFRDHVQKAQRLMDDIIDLELEKIDSIIEKINNDPEDFEIKRVER
                     KLWDNIRHKASEGRRTGLGITAEGDMLAALNLRYGSSEANDFAVEVQKILAVEAYRSS
                     VKMAEERGCFAIYDPQREMTNPFINRLRQADPEMVETMETCGRRNISLLTIAPTGTTS
                     LMTQTTSGIEPVFRAAYRRRRKVNPNDPNARIDYTDEQGDKWEEYNVFHHKFITWLRV
                     NGYDFNEVKEYDEKHINEIVAKSPYHKATAADVNYLNKVRMQGDIQKWVDHSISVTIN
                     MPENVTEETVAECYLEAWKSGCKGITVYREGSRDGILIAGKKKEKVVRQEGPLKRPKV
                     LDADIVRFQNNKEKWIAFIGLLDGCPYEIFTGRVEEDVLNIPSSIEKGQIVKIRFEDG
                     TSRYDFQYKNKYNYSTTFEGLSHQFDKNYWNYAKLISSVLRYGMPIPKVVELVGSLEL
                     DCDSINTWKNGVERALRKYIPNGTRASEAGVECPGCGDKDTLVYQDGCVICRSCGYSK
                     CG"
     gene            complement(41613..42851)
                     /locus_tag="PLK75_01420"
     CDS             complement(41613..42851)
                     /locus_tag="PLK75_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOY37936.1"
                     /translation="MLIVYRIFTGLYFLAVRIAALFNPKAKLMIKGRKNWEALLRKNT
                     DTQHSWIWFHCASLGEFEQGRPLIEKIKQMHPHKKILLSFFSPSGYEMRKNYAYADYV
                     CYLPFDTRKNAAEFFSIIRPEALILVKYELWYYFINKAAESKTKVFLISAIFRKKHWL
                     LRFKTFFAKKILLTFDWIFVQDNQSLQLLQNFECSNCSVEGDTRFDRVIQIVSEPYSN
                     TLIEIFKGNSMCLIAGSTWPADEVILSQTFRSYAGRLKLIVVPHEIHRKHIDDILSLW
                     GKNCLVYSETDNAALGNADVMVIDKMGLLSRLYRYADIAYIGGGFGKGIHNTIEAAAY
                     GVPVFFGPAFRKFNEAVGLIHCGAAFCVHNSTDLTNGIAPFLLNKNAAEIPGKIAADF
                     VFSNKGSVDRIYDKITGFLF"
     gene            complement(42859..43608)
                     /locus_tag="PLK75_01425"
     CDS             complement(42859..43608)
                     /locus_tag="PLK75_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37937.1"
                     /translation="MQRFLTASIFLLVSIPLFAQIKDSTINIWQVEINYSFQVPGGDL
                     AKKYGVNSTIGAGLTYKTRNNFMFGGEISYLFGTKIKRDTTILDALRTSNRQIINEYG
                     EYATVMFTETGFYAGLKAGKLFPIGKPNSNSGIIFQMGGGLLQHHIRIENKDNNVPSI
                     LGDYKKGYDRLCNGFAAKTFLGYQYLDNKSHFNFYVGIEFYQAWTKSRRDFNFDTMSR
                     DDVLYHDYLYSLRAGWILPLYYRNPNEFYYY"
     gene            43803..47201
                     /locus_tag="PLK75_01430"
     CDS             43803..47201
                     /locus_tag="PLK75_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37938.1"
                     /translation="MAQESINLKKTQCIISDTVQLDSLSIVDGSVVLYDENNNIIPDS
                     CYRIEYAEARIIPHKSIVGKQVTVVYRSFSFSFVKPYSHKKREHFLSSSLLGDEETDI
                     FSYQSNADGFFNSTELNRKGSIARGISFGNNQDAILNSNLNLQLSGKLSDDIEILAAI
                     SDNNIPIQPDGNAQQLQEFDKVFITVFSKRFSLTAGDFEEKKPQGYFLNYNKKVQGIK
                     FSLIDYNISKNITITSSLGGSVAKGKYRRQTIQQIEGIQGPYKLTGANNETYIIILAG
                     TEKVYINGELMQRGQENDYTIDYNTGELTFTAKLPVTKDTRIIAEFEYSERSYTRFLI
                     TSNHNLQTSKGNFWFNVYSEQDNKNQPYDQNLTYSEKLLLSQIGDNLSDAVVPNFDSV
                     GFSATEIRYRMVDTIVGAVQYDSVFVYSNDESLAVYRLGFSYVGEANGNYIKSISSAN
                     GRVFQWVAPINGVMQGNYEPVILLVTPKKKQAFSLGGKYRLSKSTVLSAETAISINDQ
                     NLFSKYDKSDDTGYAAKFQLSQRIFSTTSDSLFFSARYQFADKNFDAVENYREAEFAR
                     DWNLNDANALHFEQLAGINLNYAHKNKAHAAYSFDLLNRPGIYAGYNNKAFGGIDTGR
                     WQFNAEASFLKTSDTIFTTSFLRQKASVSRNFKFFKIGAGEEQEYNAWRYNQTDSLAG
                     NSFRWFQYRFFMQTPDSSKNGFLLAYRNRTDALPYLGNFSNSTGSHDFDLELKLLKSY
                     SQSLSVLSNFRMLNIYNDSVTQAEPQDNLTGRVEYSFKILKGAVSSATFYEIGSAMER
                     KTEFSYLEVAPGQGVYMWTDYNNNGVQELDEFEVAYFQDQANYIRYFTTTTDYIKTYT
                     NQFSQMLNIRPAQKWKNLTGFAGFMTRFSDQFAYKISSKNTADQVATYANPFANNVSD
                     TSLVSLNNTIRNEFSFNKGNPKFGFDYIFLNNRNKLLLVNGFDSRILLRHEVMLRFSI
                     RNTFQFNNLIAMGNKGYDSQYFVSRNYDIRFLENTASVYFQPNIQNRIELKYKYKNKS
                     NLTGAESLVSHDAGFEYRLSSVKRGALSFNVNYILMLFDGSSNSSVGYEMLEGLLPGN
                     NLTWQLSFQHQLANGLQINLSYNGRAAEDAKTVHTGGVQLRAFF"
     gene            complement(47184..47561)
                     /locus_tag="PLK75_01435"
     CDS             complement(47184..47561)
                     /locus_tag="PLK75_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOY37939.1"
                     /translation="MNIEELREYCLNKPGVTEHLPFDEVTLVFKVQGKMFALLPTDHE
                     LSITLKCIPEYAEELRERFDSIVPAFHMNKTHWNTIYMEQGAPEKLVKELIDLSYQLV
                     YDNLPRKLRVLKGEYAQLLKKCP"
     gene            47688..48563
                     /gene="era"
                     /locus_tag="PLK75_01440"
     CDS             47688..48563
                     /gene="era"
                     /locus_tag="PLK75_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOY37940.1"
                     /translation="MHRSGFVNIIGNPNVGKSTLMNCMVGERISIITSKSQTTRHRIL
                     GIVNGDDFQIVYSDTPGIIRPNYRMQERMMSFVSTAISDADIIIYVTDILDKSEKNSE
                     FVERVKSAQIPVIVVVNKVDLVSPAETDSVIAEWKSVFPERDVVAVSALTGYQVDLLF
                     QKIRDLLPEGPKWFPDDQLTDKTERFIVSEIIREKILLNYKKEIPYSVEVEIESFKET
                     EKIINISAVINVARESQKGIVIGHKGEALKKVGTAARIDTETFFGKPVFLEMFVRVKK
                     DWRNSDRMLDSFGYR"
     gene            48578..49882
                     /gene="der"
                     /locus_tag="PLK75_01445"
     CDS             48578..49882
                     /gene="der"
                     /locus_tag="PLK75_01445"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOY37941.1"
                     /translation="MAGIVAIVGRPNVGKSTLFNRFTEARDAIVDDISGVTRDRIYGL
                     CLWNGVDFTVVDTGGYVESANDVFELEIAKQVRIAVSEADVVLFMVDVETGITDLDQN
                     VADMLRKSRKPVIPVVNKVDNHLRQQDTYIFYQLGLGELFCISSISGQGTGELLDAVV
                     KAIPPGEKSNDNDLPRISIIGRPNVGKSSLVNALTGEERNIVTNIAGTTRDAIDTKFN
                     KFGYNFILTDTAGLRKKEKVTDNLEFYSVMRAVKAIDNSDVCLLMIDAVAGIESQDIN
                     IMNLVLRKRKGLVLLVNKWDLMQKDTNTAKSYQENIFRKTAPFTDFPVLFISALQLQR
                     VHKVLETAMQVFENRTRKITTSKLNEVVTEAVNAFHPPTSRGLQIKIKYAVQLNNPTP
                     AFAFFANHPQYIKEPYRRYLENKIRENFMFSGVPIQIYFREK"
BASE COUNT        14599 a        10061 c         9892 g        15435 t
ORIGIN      
        1 acaaggaaaa cacaaccatt atcaatggtg ttggcgataa aaaactcatc gaagcccgcg
       61 ttaatcagat taaagctcag attgaatcaa caacttctga ctacgatcgc gaaaaacttc
      121 aggaacggtt ggcaaaactt gccggtggtg tagctgttat ttgcgtaggt gctgccagcg
      181 aggttgaaat gaaggaaaag aaagaccgct acgatgatgc attgcacgct acccgtgctg
      241 cagttgaaga aggaattgta cccggtggtg gagtggctta tatccgtgca atcgaagcat
      301 tgtcgaatat gaaaggtgaa aacgaagatg aaaacaccgg tattgccatc gtaaaaagag
      361 cgcttgagga gcctttgcgt caaatagttg aaaatgcagg aattgaaggt tccatcattg
      421 ttcagaaagt gaaagaaggg aaagatgatt atgggtttaa tgcccgcacc gaaaaatatg
      481 aaaatttgtt taaatcgggt gtaatcgatc caactaaagt taccagagtt gctcttgtta
      541 atgcagcatc aatcgcagga atgttgctta ctaccgaatg tattattgct gaaatccccg
      601 aagaacaccc tgctccccaa ccgggaatgg gcggtggtat gccggggatg atgtaattgg
      661 tattacctaa attgaaaaaa gctgtcggat ccgacagctt ttttgttgaa acgattcctc
      721 tactttgttg tttttaatac gtggagttag tgaaatattt tttctatcac tccctcacca
      781 ccttacccgt acacagtttc cccccaaccc aaacattcag cacatacacc ccggcaggca
      841 gcccggcaac attcagtttc acctgcgaac ccgttgtcgt ctcgctgcat tcccgcgcta
      901 catcaaccca ctggcaaccc gtggcatcaa acaattcaat tctcgcagag ctacccttta
      961 ccggcaggct tacagttacc acatcacttg ccgggttcgg gtaaaccgct gcctgcatgg
     1021 cccgtatcgt ttgcgtagca tacccgttgt ggcaatcggg gttgcagcct tcctcaaggc
     1081 agccgcatgc atccacctta accgccaaac ccttttgcac tcccgccgga aacatactgc
     1141 tcgggggact gtaatactcc ccacccataa taaacccacc gtcgtgagta ggagttatgc
     1201 tataaaattt cgtccagggc gtattcacag aattttcggg aaagcactca taatctctgt
     1261 accactccac ctcgccctgc gggtttattt taagtataaa tccaaagtat ctagaccaca
     1321 ctgcctcgcc tgtaaggtat atatttccta agctatcttg gtaataatcg tttacccaaa
     1381 aatatctgtt tggtaggtca cttatataat cttgaatttt ttgtttctgg cttatattcc
     1441 cgttgtcgtc catccgtgca agccacaaac tgctattttc ttcattaatt gcatgatata
     1501 aatatggatc actccacgta atcagataat taccatcggg cataacaaat gcaaatggat
     1561 gataacaaat agtgtcttgt gtggctgtag tccttctcca caactcgttc ccctcattgt
     1621 ctaacctaac aaaccaggcc ctttgcctgt ctggccaatt gacaacatcg ccaatctcat
     1681 cctgacaact catcaaatag cctccggctg gcacagctat agtttggtac gggaacaccc
     1741 gccgccgata tggattggca ggcaaaaacg ggtattgctt cacacccagc gtatcgcctg
     1801 tcctgttcgt gaaaatcacc agtggaaaag agttttgact gttatagaca tacgatcctg
     1861 taataacaaa gcccaaagat gttgtatcaa ttaaatctaa tgttgctttt tgaggattgg
     1921 tggtttgtac acaatgatta taactaatta tggtatccat agttcgcatt cctaagttat
     1981 atttcattat tcttggttct aatgtatatg ctttgtaata atccacagcc gctcctgcta
     2041 taaacaaagt gtcttcaatt aaaatacatg ggcgattccc attaaatgta tagccatctg
     2101 ttgtgtccaa ataataaaac gattccgtca actcaccact tgcttcaata atattaaatg
     2161 atggcatcag gataaccgtt tcagaaagat tttctactcc aaacgatata taatggccat
     2221 tgattatgaa catttgtttt atgaccaatc cttcatacaa attgtaaaat ctttcctgag
     2281 atatggttat tagcggcatg gcaattgcca cgaaaaagaa cagtgttttt ctcatggctt
     2341 aaattattta gtaactgaaa caaccttact atcaacaatc tttccctgaa cttctatgaa
     2401 aactatataa tttccctgac taaagttcct tatatcaatc agtattatat cttcagggct
     2461 ttccagcttc ttagcaaaaa atgttttgct ctcggtgttc acaaccttga gtactacgtt
     2521 tatgccctct gttgcaatgc tgtattctac cgtaatatac ttatctgccg gattgggata
     2581 aactttcata aattcctttt ctggtagtcg gtggttcgtt ttcaactttc gattcgccat
     2641 cacatcgggc aaatacaccg gttctttata atcactggct ccgttaagca tcagcaatcc
     2701 tcgtgcataa gccccagctt gcccaccgga tgcctcaata tcgagcaatg cgtttatccg
     2761 ctcaaccccc aaacggttca acgctacgct gtcgctttct atttcctcaa gaagttcata
     2821 caagcctgca agattttctg cttcggtcat caatatcaca ttgttgcttt ctatcgcgtt
     2881 caatgcctca atggctttac catagtcgcc ctttccgcca taataagctg ccagcttcat
     2941 gccgtattgg ggttcctggc ttatactaag caactcctca acatcgccat cattatatag
     3001 ttccgggtgt cttaggtaat agcgcagttg ccgcgatgtt ttctcgctca gctccgcgct
     3061 cagctccgcc cgctgcattt ccagatactc tttcggcgaa atctgcgtca gcccgctgtt
     3121 tatctggtct atcatatact gcggcaatgc ctcgatgcgc tcatccaaaa tcttttgtat
     3181 ttcgggatcc ttagatgcct gcggattggc taccagtaca tcgcgcacca tcggttttgt
     3241 tagggcatct tctttcagtg caacctcttt cagcgcctca ttgctcaggt aaggcgattt
     3301 atccatcagc cccagatagg tctgccatgc gttcaaatcg ctggcaagca caatctcggc
     3361 tatggtcagc ggcgtgttgc ctccgtcggt cagtgcttcc agcgtgttgt caatctcgtc
     3421 aatcccccca ctcaacatcc gcagttcagg cagtataatt atcgtatcgt tgcctgtggt
     3481 atcggtgttg tccagacagg tactgtctgt aaaataaacg ccagtaggaa ccctattcac
     3541 ataccctaca atcgaatcgg ggtaaaaacg aatgttagtc gatgtgctat gataaaaata
     3601 attaatgtta tggtcagaag tgtttatcat attaatgtat gcattccctg tgccccaatg
     3661 cgtaaacttg ttccccgcag ggtttaacat gctcccctgg ttttcggcta tccctacggg
     3721 gtatcctgga ggataagggt tgtcgagatt gttggtaaca aacacatctt catcgttata
     3781 ttgaaaattg ttacacctaa tctgtaaacc tgttggtgcg ctcgagcttc tgttcaaccc
     3841 aattgcttca atgctcaccc gcatgtgctg tatattattg cgatatatct tctcctgatt
     3901 gctgtaaaca tcgttcacaa tcaccccgtt aataacatct gaagctcctg cttgaaaaac
     3961 atacaatgtg ttgccttcta actgaaaatc gtaactattg tctagataca cacaataggg
     4021 caaaacctct gcatcggtcg agtcgcagtc aacaaaacgc ataatattgt ttaccaccct
     4081 ggcgccgtgc gattgtgata gatacacccc gtaatggcag gtttcaaaca ggttatcttc
     4141 aatgtcaata ggtaaatagc tgccgttgta cgcacgtatt gccgaaccca aattaccaaa
     4201 ataacaggct tcatccaccc taaaacccga cttatatgtc caaatcccca tcctattgtc
     4261 ttcattaaat aaaccattat caaccccatc atcaataaac cggcaatttt taaactgcac
     4321 ccctttcgta tttaccaata tcacattcgg ggcaagctct tcatagtgct gttcgttgtc
     4381 tgtcgaaaaa tgacaatttt caaacaccga tgtattgttt cccaaaacct gatcataata
     4441 atcagacatt tgtagtggaa caattagaac gtcgcaaaag ttgtatttaa aacaagaatt
     4501 attagcccta ataatagcac cggcatcact acaaattcca tattttgcat cttcaatgta
     4561 cgattgtttt atcataacta aagaacgccc tgtaatacca agtgttgggt ctaaatttgc
     4621 agttatccta aacccttgcc acaaagtatc gcaaccatta aaaatgttgg cattctcaac
     4681 ctctatggtg gtccctattt ccgattgaat atatacattc tctgtgcatt gaatatcccc
     4741 ctgcaaagta agcatcacat tcgatccctg aacaattatt gggcgtgtta ttatcgtatc
     4801 tttcagtatc gtattgcttg ttatatatat aggtacatcc gacttccaag cctcaagcga
     4861 gtcacatgcg cacacacact gcaaatatgc attataatgg tgttggttag gattgttgtc
     4921 gttgcaatcg cgcaaatcag gccccggaca acccgctggt ttttctccaa taccccagtt
     4981 gtaatatcca tcaccgtcac catcaaaata taaaggctct aaagtttcat tcataaggtc
     5041 tgcaattggt gtttcaaaag caaatgcatc gtttgaagta ggcattgaat ttaatttccc
     5101 taatacatta aaataaccca taacacctct atctaaactg gctgattctt tgaaaatcca
     5161 catggtacga ccaataagct tattacctgg atatattact agtgtatcag gaataaataa
     5221 atgatttatt gtatcaactt ctaccctcca accgtagttt atattaagtg tatcaaaggc
     5281 ttgtatttgt ttatacccaa ccaaagtagc tatgtgattg cttttaggtt ttgcagataa
     5341 agggccgtaa attaccaaag cccttttaac ttcattttca ctatctccta ttgtaatcat
     5401 ttcattaata tctatcttaa aagttgccgt tgaaatatcg cagcaataac ccgtcataga
     5461 atcattccaa ccaaagttgc tttcatcaac aaacagataa ttttgtgtat tgataaaatt
     5521 gtccaagcta tctgttttcc caccattaat acaatcgcta gttggcgaat aaacattaaa
     5581 acaatcggct aagtgttgct ctgatatatt ataatcgagg tgctgattaa aatatagatt
     5641 aattactgct tcaagagagc caatcggagc aaataaataa caaccattgg ggcaagaacc
     5701 ccatgtatgc atgtctttta cggaagtaag ccaaccattg ttttcattta ccaaatacgc
     5761 tgtattttca atgtttatta ttccataatc tggattttcg tcaaaataag gtgaagtatt
     5821 cagatttgct ccatgagctt ttcgccaatc aaaacttttt gcttttaccg gaggcaacgt
     5881 gtcgatagta cttttttcac caatgttttc attgataatt tcaccccatt gcgaatattc
     5941 gcagaaaata cctccggcat agtagtcgta tccatcaata aaatatttat cgcctcccca
     6001 atattgcgct tttttagaat aatctgccgt attcattttt gttacccttg ccctccaagg
     6061 caatcccttt atttcaaggt tttcatttat ctggtcaact ttctgacttt caatagtttg
     6121 cttgtattgt tgcgctaaaa caatgtaatt tagaacaatt ttatttgaaa atgccattga
     6181 ggaaaaatta cattctgcat cctctatata aaactccaaa atatcgggat ttgtgttata
     6241 cggcatatat ttggtttcgt cataataagc ggtaaaatga accacagaat cactacaaat
     6301 taatggatag gcttctaaaa gtagtcttcg ttttcccaat gcatctacca ttataattcg
     6361 taccaaactc gtatcggaat gtaaggttac ggttgctgta acgctagctg cactacattt
     6421 acctaaaaat ttagtattca cgttgatagt agtatcttta tcgaccaata gattgaaatt
     6481 aatagtttgc gcctgcaaac ccgatacaat cgttgttacg aaaacaataa ttaataaaaa
     6541 ctttttcatt ggtaaaaaat ttactgaggt aattatatgt tcacaagtta aacaaaatat
     6601 tatccttaat tgtaatttat tttaatcaat aaagtgtaaa aatgcagaca acagtatgag
     6661 tttattgttg ttttattgat tcaaagcgta ttaattcaac acctgttttc tcccataagt
     6721 gcatttcgct ttccaaacgc ttttttagtt tttcgtaatc aagataaaca tctccacgag
     6781 gttctttgta tttgtccgga ctgttgataa aaatctctat atcattaatt tgttgttcta
     6841 aaagttcaat ctgcttttca tgattatggt gctcgtttcg aatttttctt atcgtttttt
     6901 ccagttcctt acgttcgatg taaatttgtt tttgttctgt gttttcttct ttattttttg
     6961 tgggaggctt gctgtatgaa aaaagctgca tttgaatttg ctttctgtat cgttttatat
     7021 atgcattaat gcccccggtt tcttctttaa tttttgtccc cattacctcg taaaccttat
     7081 tcaccagtcc gtttaaaaaa tacctgtcgt gcgacacaat aagaagagaa ccgtcgtatt
     7141 tcattaaagc atcccgcaga atgtctttcg attgcatatc gagatgattt gtgggttcgt
     7201 cgagaataat taagttgtat ggttcaagta tcagcctagc caaagcaagt cgcgattttt
     7261 cgccacccga tagcacctta acctttttat cgatatcgtc gccccgaaac agaaacgagc
     7321 ctaatatttc tcgtaaacga gaacgaatat ctccaatggc aacatagtcg agggtttcga
     7381 gtacagtcaa ctcaccatcc aacatttctt cctgattttg agcatagtaa ccgatgtgca
     7441 ggttgttccc tttttttagc gttcctgagt aatcgagttc tcctgcaaca attttcgaga
     7501 aagtagattt tccctctccg tttctaccca ctaatgcaat cttgtcgccg cgttccatcg
     7561 aaaaatcaat ctcgttcagt acaatgtgcc cggagtattc tttcccaagg ctaatggttt
     7621 cgaatactat cgacccggaa cgaggtgatt gaggaaatcg gaaatgaata cttttagtat
     7681 ctatttcatc gaagtttacc agttcaattt tctccagctg ttttatcctc gattgtacct
     7741 gatttgcctt ggttgcttta tagcgaaacc tgtcgatgaa ttcctgagtt tcggtaatca
     7801 ttttcatttg gttgttgtag gccgctttct gctgcttcaa cctttcttcc cttaatgcaa
     7861 caaactggct ataaggaact ttgtagtcgt aaagttttcc aagcgataat tcaatggtac
     7921 gggttgtgat attatcgaga aaagttcggt cgtgcgaaat tatcagaaca gcccccggat
     7981 actgttttaa aaactcttca agccattgga tggcaataat atcaaggtga tttgtgggct
     8041 catcgaggag cagtacatcg ggttgcaaca aaagaatttt tgccagttca atacgcatcc
     8101 gccaaccacc actgaattct gcagttttgc gattgagttc agcgtcagtg aacccaagtc
     8161 cctttaatgt ttttactacg ctagcctcgg tattgtcctg ttttaataga tgatacttgt
     8221 cgttagctaa cgataaactc tgaatgagct tttcgtattc cgaactgtga tagtcgtttc
     8281 ttgaagaaag agcttcttgt aaggttttaa tctctctttc caactctttt atttcggtga
     8341 aagcctcttc agcttcttca aacaccgacc ttccgctgtc ggatacattt agatgctgag
     8401 gcaaataacc gattctgatg ccagatggta tgctgacact accggaagta ggttgaattt
     8461 gtccgttcat gattttcagc agagttgttt tcccagctcc attttttcca acaaggccaa
     8521 tacggtctct gccgttaacc ataaagccaa tattagagaa gagttcaaaa ttcccaaaca
     8581 tcagacttat gttattaatc gcaatcattt ttatgggcac aaatataatg attacaccaa
     8641 atatcaaagc ttttcgaaac agagtgtgct ataaaataat tactttcgtt catcttaatt
     8701 tcgatgcata tgacacacag gattgaacac gattcattgg gcaatattga agtgccttcc
     8761 gattgcttgt ggggagcgca aacgcagcgg tcgttgaaca atttcaaaat cggaagtcag
     8821 ctcatgcctt ttcaggtgat agaatcactt gcaatcatta aaaaagcttc ggctattgca
     8881 aataattcta tgaatcttct cagtgaagaa aagaaaaacc tcatcattca ggtttgtgat
     8941 gaaattattg caggtaagca tatggctgaa tttccgttgt ctgtttggca gaccggaagc
     9001 ggtacgcaaa ccaacatgaa tctgaacgaa gtaatttcga acagggctca ggaattgtcg
     9061 gaaataagtt caacagaaaa gggaaagaag ctgcacccta acgatgatgt aaacatgagc
     9121 cagtcgtcga acgatgtttt tccggcagca atgcatatag cagcctatac cgtactggta
     9181 aacacgacgc ttcccggttt gaaacaactc gcggcttcat tgaaaaaaat atcaaaccaa
     9241 cacaaacgtg cagtcaaagt tgggcgtacc cattggatgg atgcaacacc aattactgta
     9301 gggcaggagt tctcgggcta tgtagccctt attgaaagcg ctgcagggcg aataaagaat
     9361 gctgcatcac gcttgctttt tctcccgttg ggagccacgg ctgtgggtac cggtttgaat
     9421 gctccgaaag gtttcgatgc gaaggtatgt tccgaaatcg caacactgac agggttccca
     9481 ttcaaacctt cacgcaataa atttgaagct ctttcggctc atgatgcttt ggttgaagtg
     9541 catgcagcac taaagcaggc tgcagtcagc ataacaaaaa ttgctaacga catcagatat
     9601 atggcttctg gtcctcgttg cggattaaat gaactgttgc tccccgaaaa tgagcccggt
     9661 tcttcgataa tgcctggaaa agttaatcct actcaggtag aagctatagg tatggtttgc
     9721 tgccagataa tgggaaatga tactgttgtt actgttgctg ccatgcaggg cagctttgaa
     9781 ctaaatgtgt acaagcctgt tataataaga aatacccttg aatccgctgt tttacttggc
     9841 gactcctgcc ggtcatttgc tatgcattgt gtagatggta taagagtaaa caatgacaaa
     9901 ttgaagtact acctttcgca gacattaatg ctaaacactg cgctgagcaa agtgattggt
     9961 tatgaaaaag cagcacaaat cgctcagata gcgatgcgtg aaaaccttgg acttaaagat
    10021 gctgcaataa aatcgggcta tgtctctgaa gccgattata acagatttgt tgatgtaaat
    10081 aagatgattt aataaaatag aaatatacta tatttgacaa ttaattcttc agtatatgtg
    10141 cggtattgcc ggcttctaca caaacgcaga cactttcgcc tcgagagatg agcttgataa
    10201 aatggttgca gctttggtgc atcggggtcc tgatgccagt ggcgtttatt tttcgaatac
    10261 tgtcgggttg ggacaccggc gtttgagtat tctcgattta tcgcaggatg ccaatcagcc
    10321 aatggaatct cattcgatgc gctatgtctg tgtatataat ggagaagtgt ataatttcag
    10381 aagcattgcc aaagatctta atattgattt taaaaccaca accgataccg aagttattct
    10441 cgaagctttt gctgaatggg gggttacttt tattaacaaa ctgaatggaa tgtttgccct
    10501 ggctattttc gataaacagg agcacaaact ctatttgttt cgtgatcgca ttggtgtaaa
    10561 accaatatac tactattggg atggacggaa ttttgctttc gcatctgagt taaaatctct
    10621 tcttcagatt aagtcggtaa gagacagcag gcaaatcgat tttcagtcga tttcacatta
    10681 tctacatttg gggtatattc ctgaaccggg atctatctat aaaaatattt ataaatttcc
    10741 ttcagggaat tttggaattt tatcggaaac cggcttgaga attgagtcgt attggttgat
    10801 agaaggagcg gtgcgccgac aattaattga ggatgaagta gagtgcaaac aaaggctcaa
    10861 agacctgatt gaatcgtcgg tttcgatgcg gttgatcagc gatgttcctt ttggcacctt
    10921 tttaagcggg ggagttgact ccagtcttgt aactgccgta gcaagtaagt actcagcaaa
    10981 aaaactgaat acattcagcg ttggatttta cgattctaaa tacaatgaat cgacctatgc
    11041 acgtagggtt gcagagtatc ttggtaccaa tcatcacgaa atggttgtaa ccgagctgga
    11101 tgcacagcat atggtactcg acctgctgaa ttattacgac gagccttttg ccgattcttc
    11161 ggcaattcca actatgctga tatcgaaatt tgctcgcgaa aatgttacca tggtgctgtc
    11221 gggcgatggg ggcgatgagc tttttcatgg gtacggcgca taccggtggg caaggagatt
    11281 gaattatatg cccacaagac ttgcccgcca attaataggt tttgtacttg ccaactcggg
    11341 taataataaa taccagcgcg ctgcacattt attcaggtat caggaacgca aacacctgaa
    11401 aagccatatt ttttcgcagg aacaatattt gttttccctt gccgaaatta acaaaatgct
    11461 actccatgat ataaaaattg agactacagt ggatcaggat tattcatatt tcgtccgcaa
    11521 gcttactcca gccgaagatc aggctatttt cgatctgaag tattacctca aggacgatct
    11581 gttggttaaa gttgacaggg cctcgatgaa atacagcctc gaaaccagag ttccattact
    11641 cgattaccgg attgtggagc ttgcactaaa tatttctcca aaactgaaaa tacaaggcag
    11701 tgttcaaaaa taccttctaa aggaagtgct ttacgagtac ttgccacgtg aatatttcga
    11761 cagacccaaa tggggtttct caataccttt aagcaaatgg ctgagtaaag atttatcgta
    11821 cttaattgat gaacatttga atgaatggat gataaggaag cataaacttg tcaatttcga
    11881 ggaaacaaat aagctgataa aatcattccg gtcgggcacc gattatttgt acaatagaat
    11941 ttggcaactt attatattgc acaggtttat ggaaaaagac aaaaacatac caattctgta
    12001 gtttaagaag atgtttttta ctcatagtca tcattttcag tcatccattt tgcatttgcc
    12061 ataataattt cgtcaaggtg tttgccttgt tcccttgctt gctttttcat aagatccagc
    12121 cactgttcac tgttctttat gctataaacg taatattcaa ccgatccgag aggcattgtt
    12181 ttggctcggt atacagcttc ttcgtagaga acgtcattta accggcgttt gcttttcttt
    12241 gctttttcga aaatactgat aaactgtttg cggtcttttg ttatatcgtt ctgataatag
    12301 aaaacagcgt caggagtata atcaggatta aatagtttaa aagccatatc ttcaaaattc
    12361 caaaatgcag tatgcataaa tgtctctgta cattcaagca caatcacatc acgcttaaga
    12421 agttcctctt tgatgttaac actgtaatta tagagttctt tctggtagaa ctctggataa
    12481 accatcacat tataatacca taaattataa tgtcggaata agtattcacc gagcttatac
    12541 ttcagccaat gaaacataaa actatcgcca acaataagaa tatcaagctc tgggcgtgta
    12601 gtatcgcttt tcgaaaactg cggatatgcc aaagtttttt caggcaaggg gagcatgagg
    12661 ttcatgattt ttcccacatc ataatcctca ccttggggct tatttgtctt ttttaatccc
    12721 gatatagtaa gttcaggtat tttttttgat gatatttttg ctgtataatt taaaaatgta
    12781 tccaaagcaa gatacattcc gtattcactc caatggattc catttgttgg gaaaagagga
    12841 tatgacacgg tgtctttgat ttcgtcaaag tattgttgca gatcaagtgt cctgatatta
    12901 tacttgtcgc agtttttaac aaagcatgta tagttatttt catcgtattt cgagcgaaga
    12961 taccggtcag gtatattttc gggcataaag gatgctttgc ctggtgcaaa aaccacaaac
    13021 atggtaatac ctatttgttg aaaagtatcc tgaaggtatt tgattctttt gcatctttct
    13081 tcaattaaat tattgcccac gaaataatct ccggtatgtt cgagaatata acccgtttgc
    13141 agacattgat tgttttttcc caatacaacc tcagcagcat tggttttacg aaatacacgg
    13201 tatttgtatt cgttatacgc tctcagcatg ggagggcgaa gtttgaaatt gttgtcgata
    13261 taggtactta agctgtcttg aaatgattca tcaatccatg ttttagcgct gaatgccggt
    13321 aatgaatgtt tacttgtaaa tccttcaagt actttgaagt tgaatttaaa caggccactt
    13381 ccatcgatta tcagtgatga tgataatata agtaataaaa cgaaccaaag aatatcttta
    13441 tatccccgtt tcatcagaat ctgaagtaaa tgaaaggatt aaacccggaa gcggaaatgg
    13501 acgctgtaca aacaaataaa agaaccattg caacaattgc catgatgaaa tatccggatt
    13561 ttgtgtattc ggaatataga attttctttt cgaggttgaa cccaaaacgg aacgctgtta
    13621 ggaatgagaa aatcaatgct acagccagca ttacccagaa ttctctgtcg aaatactcaa
    13681 aagtgctgaa atcgaacgca aagagccgcc cgatatatgc aaaagcaaac ccaagcgttt
    13741 cgctgcgaaa caaaacccaa ccaattattg caacaagaaa ggtaaaaatt atgcttgggt
    13801 actttccgat tttagccgat aattttaaca gaaacaaacg gtcgagaatt aaaaataaac
    13861 cgtgaaatgc accccatgca ataaaagtcc aggaagctcc atgccagaat cctgatatta
    13921 ggaacacaac ccacaaatta aaatacaggc gccacttatt gttaacacga ttgcccccta
    13981 ggggaatata caaataatcg cgcatccagc gtcccagagt gatgtgccag cgacgccaga
    14041 attctgtaat gttttgacta atatacgggt tgttgaaatt ctcggggaat ctgaacccga
    14101 tcatgcgccc aattcctatt gccatatcgg aatagcccga gaagtcgaaa taaatctgaa
    14161 aagtgtatgc gagaatccca atccaagcgg tgcccgttga catgtcgatg ggttgcatgg
    14221 cgaatattct atcggcttca gcgcccatta cattggcaat cagcactttc tttgccaatc
    14281 caataacaaa acgataaaat ccaagcaaac gattgtcaac ggtttcatag tcgtggcggt
    14341 tgtgaagctg atcggcaatt tcgctgtacc gaacaatggg ccctgcaatt agctgtggga
    14401 aaagaagtat atataagcac agatcccaga cgctatcaag aggcttgtgc ttcccccgat
    14461 atacgtcaat cccgtaggtg aatttctgaa aagagaaaaa agatatcccg attggcaggg
    14521 caatctttgt ccaatgcaat gaattcgcac ccaatgctcc taaaataaca ttgacatttt
    14581 caacaaaaaa attcatgtac ttaaaataca cgagcaatcc aatattcaga accaccgatg
    14641 cagtaatcag ccagtgtcgc tttttcccac ccgaggcatg cattgcctta atcagataaa
    14701 agtcaataaa aattgaacca agtacaacaa atatgaataa tggagctccc caggaataaa
    14761 aaatcaaact ggcaacaagg gcaaccagat tcttatactt tctatccgat aaataatata
    14821 ataataagaa aatgggaaaa aaatagagaa ggaatatgct gctgctaaat accactttcc
    14881 gttaattata ttgttattat tcctgagaat tttaatgggt aaaaataggg aaatgttcag
    14941 aaaaaacaag tataaaaaaa taggctaaat gagtaaaaat gcaactattt ctggagttgg
    15001 cgggtactat tcgggctttg ttgcactgga gggaaatgaa acgcatgcat gaatttatgg
    15061 gcatttaaat tattaacata caattttacc cttgattaaa aaacaatttt caactgtgta
    15121 tctgtaagtt taaaactcat gcgatgccat gtggttggcc cgaactgttc gatagcccgc
    15181 ctatgtttta ttgttggata acctttgttt tgcttccagt tgtattgcgg aaaatctgta
    15241 tgcaattttt ccataaagtc gtcgcggtat gtttttgcaa gtaccgaagc agcagcaatc
    15301 ggcatgtata ttccgtctcc ttttacaatg caggtgtatg gaatgttgcc gtatttttta
    15361 aaccggttcc cgtcaataag gatatgctcc ggctggatag tgagtttctg tagtgcttta
    15421 tgcatgcatt cgattgatgc atttaaaatg ttgatctcgt caattcgttg gttcgataca
    15481 ttagctacgg caaaggccag agcctcattt tcgattattt ggcgaagaat actgcgttgt
    15541 ttatggttta gctttttcga gtcgttcaac atttcgttgc ggaaattctg aggtaaaact
    15601 actgcagcag caaacaccgg tcctgacaaa catccgcgcc cggcctcatc gcaaccggcc
    15661 tcaatcaaat catgatgcat gcatcttagt agcatgttat cgagttagtt tttcgatgga
    15721 catccaaact ctttcggcgg ataattccca tgagaatttg gctctctgca gccggccatt
    15781 ttcgataagc ttgtttctca atatcgagtc ggtttcgata agaagcatat tactagcaat
    15841 gctttcgtaa tcgtttgggt cgctcatgag cgctgcatct tccactactt ccggcaatga
    15901 tgttacattg ctgcataaaa cgggagtatc gcaatacatt gcttctacga gcggtatacc
    15961 aaacccttcg aaaaatggaa taaacaccag cgaaatggcc gatgacatta ctttactgag
    16021 ttcgtccacc ttgagccttc caacgaaatg aacccttttt gcaaacttca ttccattata
    16081 gcattctgtt atttcttcag ttttatgcaa tgcgtctccc acaataacca ggctgctttc
    16141 accacccatt tcgcagtaac ggtcaaacga cttaagtaaa cccggaacat tttttctcgg
    16201 gctcaaggct ccaacaaaaa tgaaataagg cttccctcga gtatatttat tacggatctc
    16261 ctgctgcaaa acggggttca ttggtcgata agcgtcgttc gctccattgt aataaacatc
    16321 gattcgctct tcgctcactc cgaacgattt tgaaatatcc attcttgaat attcggaaac
    16381 cgtgccaatt cgagatgctt tttttgcaaa ctttggaatg aagtatgtaa agtaacgggc
    16441 agctaagcta ggtaaatatt cagggtgata aataaaattg atatcgtgaa taacacatat
    16501 tgtgggtact tttgttttca gcgaagcata gccatcgggt gaaataaata aatcagctcg
    16561 tgtgtttcgt aaaatagcgg gtattatcca ttcaaaccac atgtaccaaa gcaaaggatg
    16621 acgtgttgga ggccctttta taattatgcg tacattctta ggaaaaagca atgaaggatg
    16681 cacaggcctg tcgaaaataa agataaagtt gtgctcagga tggttctttg ccaatcgcga
    16741 catggtttcg taagcaaacc aaccaattcc ctccatcttg tcctgaatga caagtcgcat
    16801 atttacagca atgttcatca gatgaggcct atttgtcgga tttgctccag atcttttgga
    16861 gtatcaaccg atatactttc gtgttcggta aaagcagtct tgattttata cccgtattca
    16921 atccagcgaa gttgctccaa cgactcggca agctcaaggg gggaaggcag cagtttggtt
    16981 atttcggtaa gcactccact acggtaggca tacagtccga tgtgtttgta atactgatgg
    17041 ttggttatcc atcgcaattc ttttactcct ctgataaatg gaatgggaga tcggctgaaa
    17101 tatatggctt cattcttttg gttgataacc acttttggtt tgttgggatt gaaaatatcg
    17161 tcctgattgt tgatgggctt aataagtgta gcaatctggg ttgacttgct tcgaaagcat
    17221 ttttttattt cattcagttg ttcaatctga ataaaaggct catctccctg tatgttaacc
    17281 accacatcga atgatgtttt taacgtatcg ctgataatac ggattgcttc agcgcacctg
    17341 tcagttcctg aacgatgttt tttcgaagtc atcaccacat tcccgccaaa tgaattcact
    17401 gcatcaaaaa ttctagtgtc ctcggtagcg acatatacaa attcgaatgc tttaagtgcc
    17461 tgctcataaa cgcgttgaac cataattttc ccatttatca aagcgagagg cttgccggga
    17521 aagcgcttgc tcccatatcg ggcaggaatt attgcaataa acttcattac agttaaatat
    17581 ttgcaataaa aatacataat tttagcaatt caatccatat tgaattgttt ttaatgaaac
    17641 acgtatattt atgacgaacg acagccataa taaaatgcaa aggcaaatga gtcctaccag
    17701 aatcatttta cccatgctga tagggcttgc cgtagtggct tttttcatta tccgcgattt
    17761 taaaccagga atgttttctg acgtttcttt taatacaggg gtcggaacaa tgctgctatt
    17821 ggcatgtgtt atgatgtttt ttcgcgattt tggctacgta ttgcgaatac gatatttgtc
    17881 cgataaaaaa ctctcctgga aacaaagcat taatattata attttatggg agttttcttc
    17941 cgcagttaca ccatcggctg tgggaggaac ttctgtggca acgtattttt tgtggaaaga
    18001 gggtttttcg gcgggaaaaa gtgcttccat tgttatggca acttcattcc tcgacgaatt
    18061 gtactttaca cttatgttcc cgatactttt cctgattttt tctcacacag ggcttttccc
    18121 ccttgatgaa aacggtaatt tgaacagcag ttttcttgta tttgctggag tgggttatgg
    18181 tataaagtta ctttggacat tggttatggc aacagcactc tttgtaaagc ctgttgctat
    18241 gaaaaatatt ttaaatacat tgtttaaact cagaattctc cgaaaatgga aagaagctgt
    18301 agcgcatgct ggcgaagata ttgttgctgc atcgcatgag ctgagacata aaacaatagg
    18361 gttttggatt cgttcttttt tagcaacttt gttctcatgg acatcacggt attgggtgtt
    18421 aaatttttta attcttgctt tggcattcgg attaccatat ttcgatgcgc acaccctttt
    18481 ttcagtcggg gagcatttcc tcatttttgc acgacaactg gtaatgtgga ttatgatgtt
    18541 ggtaatgcct acgcccggtg gcagtggttt tgttgaggct gtgtttacca gttatatggc
    18601 cgatttcata ccgattgtgg ggtttgtttc tgcaatggct ttgctgtggc gatttgtaac
    18661 ctactatcca tatttgattc ttggggcaat tgttgctccc cgctggtttg caaaatcaag
    18721 gactaaaatt ggtaattaac gaatacttgt ttgtgaatat ttattactta tggaaaaaac
    18781 aaaaattgga cttgcctgcg atcatgcagg ttttgcagtg aaagattttt tgcatggata
    18841 ccttgaggga aataattacg aggtgattga ctatggatgt ttttcggaag atagctgcga
    18901 ttaccccgat tttgcccaca aactggcttt gggtttcgaa aatggggaat gtgttgccgg
    18961 tattgctatc tgtggaagcg gaaacggcat taatatggca ttgaataagc accggtacat
    19021 ccgggctgct ttgtgctgga acgaagaaat tgcaaaactg gcgcgacttc acaacgatgc
    19081 caacatttgt gctatacccg gcaggtttgt cgacgcgatg cttgctgcaa atattgtaga
    19141 tgttttcctt tctacaccct tcgaaggagg acgtcaccag cgccgaattg ataaaatccc
    19201 ggtgcattga tttcttagaa aaaaatctcc caatatcggt ttaaaaaaat catatgaaag
    19261 aagttaatca aactggctgt tgtccccctt tcgacccgaa accatggaac aacaatactc
    19321 atcattggaa taacaagcgt tttgtaaaag accatgtaat tactgagcac tgggtgccag
    19381 taaacttcgg agaggttgtg caacgcatta tggaaaaaat ggatgctgca ggagcgcagt
    19441 cacccggatg gatgggcctt tctgatcata cttccgaaaa aaacatggat atttatgttg
    19501 aaagcgatcg cgaagttccg ggaattgaaa atgtgagcat tagtgctacc atattcagta
    19561 aggtgtatga tgggagtttt gataaaacac agttgtggtg cgacgatttt aacaaggaag
    19621 cacaaaagag aggctttcag ataaagaaat cgtatatgtg gtacactacc tgccctaagt
    19681 gtgcagaagc atatggtaat aacgtgatgc aagtaggcgt atgtagacct gtgttgcgcc
    19741 tgcggcaggg ctattacgct tacttgcggt tatgggctgg ttatttttaa atggttactc
    19801 ctaatatgcg aaactcaaac gattgtaaaa ccgagtatcg ttttgacgac aacaatagga
    19861 ttgcccaaat ggcataaaga ataatgctgg ctaatagaaa caataaccca atcccacacc
    19921 aacattaaaa ctcgatccat tactagaaat ggtataatta ttcatcactc catcttcgtt
    19981 aattgaaatt ctgttattct cccaaacttt atttatccct gaataataat taacgataac
    20041 atttatttca aacttagatt ttactaaata tgaaatttca actccattga taaatccaaa
    20101 agaccaatca tttttgaaat tataatctga aatagtacct gttaagctgc ttgtagaagc
    20161 accaattaca ccattataat ggtacgaatt gcagaatgct attgaaagac cattttcaat
    20221 atagatgctt aagtttttga ttttcaaaac atttgctttt aaaataatgc cgagtggaat
    20281 gtcattgttt tgaaaagatg aaattaaaga atttggcaaa gacagatggt tttgactata
    20341 accaatgcta gctttaaaat aatatgtttc gttaaattta tagtcaaaga aaagaccata
    20401 ttcccaactt tgaaacatct ttctatctga ttttaagaat tcagagcctt tcaaatagga
    20461 ggcaacttga ttactgggac aaaaatatgc cccgatttta aagttttgag aaaaaacgaa
    20521 aatgctgatt tggcataatg cgagaaataa tactattttt ttcatattta taaagtttta
    20581 attgcaattc ttctgatgta atgagagagc attgttacct gtacttgttc catcattgtc
    20641 ttttacataa taaccagaaa ataaaccatt tttccttact cttaatggag tgtgtgtttg
    20701 ataataagta tatgttgctt tatttttatt atattttact tctccttgat caacagcggc
    20761 ataatctgta cagtttgttg cataaacaaa tccggcaata gtcgttgtta ttttatcggc
    20821 tttctctaat gctaagcttc cattgttttt taccctataa tttattgttt tgcttgcaaa
    20881 cccatgcaca ggccaaacat ttacaatttt aattaccaat tttgcttttc gtgtgccgct
    20941 cgcataagtc cttgttgtgt ctttttcttt atcatacatg acacaacaat ctcctgaacc
    21001 agatacgtga agtgttgatt caaaactatc tgtgcatcca gcatcagtag aaataactaa
    21061 ttttacaata taagttccat ctgtagaata agcatgtgat ggactttgtg tggttacatg
    21121 aggagtgtta tcaccaaaat accaatcata agaaacaata ttgccaatgg aggcagtaga
    21181 tgtattagta aaatagtact ttccaacaat atcttgatca gaaagagatg tgaaatttgc
    21241 gttgcaactt ccaactaaaa tatctttctc tgtggaaagc ccgctccccc cataggtgac
    21301 tgttagtttc acactaaact caccagatgc tgcaaaagta tgagttggtt ctctctctgt
    21361 tgaagactga ccatcatcga attgccaata ataagaaaaa tgatttggat tttcattagt
    21421 ataattaaca ataggtgtaa agcggtatga caatgaatta ttattaatat ttacataata
    21481 aaaatctatt accattgttc gcaaatccat gtctgctggt agcgttacaa tatcccgttg
    21541 taagcatagt tctgcaattc cagagacccc agacttcaaa tatgccccat ggacatctct
    21601 tagagcgtcg taattataat tatgaattcc tactgttata gaatctaaat aaacacaaat
    21661 aatatcttct actattagtt cgcgatttgg ggttaaaatt gaacgaaagt catcgccaac
    21721 aattttgtca ttatcaggat cattaaactc agttaacata tcaaggtttt ctagatagat
    21781 tacttcttga tctattgcat ttctgagaga tttgaaacca taatgatact cgaaagcaaa
    21841 taatacagga tcgtttgggt aaatgtcttt ggggtcatca agtttatttg catatcgtga
    21901 tagtatgttc aaaactgagt ctaaatctga aattttatca aattcaaaga tacctttgtt
    21961 gtttaccttg aaagagtatg gttttaatac attcaacatt tctattacaa tttcttcttt
    22021 cattaggctc tcaatatcat aagcactagt gtttcttaga ttgcttgtct ctctacttga
    22081 atcttctttt tggcatgata ctacagaaat aattaaacta aacaagaaca tttttaataa
    22141 taatccttta ctttttttca ttctattaga gtattaagtt actgcctact tcgatttatg
    22201 gtctcattct gcaaattccg acccgctatg ttcaaatttt tcgcagtcag cattgattct
    22261 tatttattcc gaaaaagtcg gtgtctttac aatgcatttt tctttcttaa cttgcccata
    22321 actatgtagt aattttagga ataatagcag aaaactaaga cgtgcctatt ttttactgct
    22381 cattttaaac aatttttctt tttccgaagc actcaggctt ccgtaacccg accgggcaac
    22441 tttatcgaga atggcattaa tttcatcttg atccgctttt ttacgagcat tatattccat
    22501 atcatcatac ttcgagtatg aggctttgtt tttattgcgg cttgttactc taattttctt
    22561 tttctttgaa aagataatta taatgccatc tgttattttc aaaagccatg aaactatatc
    22621 cttgcctttg cggtattgca gcgagaagat aatccctata aaagcccctc caaggtgtcc
    22681 gatatgccct ccggtatttt gcagcggaat gctgatgata tcgatagcta ctgccgttag
    22741 accaatatat agcagtttaa tctttccgag tatgggaaca taaacgtcat gtttgggtac
    22801 aagtgaaact attgcgaata ttatagccat taccgatgcc gacgcacctg tggctggcgt
    22861 tatagcgggg ttgtttccca atacaggaat agtgttatac gacaatacaa atgcaagtcc
    22921 accggccagg cctcctagca gatataaacc aagcatttgt ctttcggtga gatactgtag
    22981 aaaaacttta ccaaaccaat ataagaacaa catattcatt aaaatatgta aaaaactgaa
    23041 atgggtaaac atgtatgtga ttaaagtcca tggatgggta atgaacccta gtaacgaagg
    23101 aggtaattca aaaaaggatt tgaattccga taaattcaaa ttcggaagat acaagaccgc
    23161 taacgtcgaa atcaacaaaa agaacaccag cagattggtg tatatcaatt ttgtaaggat
    23221 agtccctttc gtgaaggaat cttttatttc aacaaaaata ttcatcagtt ccagcgatta
    23281 aaccgatgtt tcttccaaat tagcagaaat atcagaccaa ataacatccc cccaaggtgt
    23341 gcaaaatgag ctacattgtc accggctctg ttggcaaaac ccagatataa ttccaaacca
    23401 ccgtaaatca ttacgaaata tttggcttta agcgggagag caaaaaatat ataaataatt
    23461 gcatttggga acattacacc aaaagccagc aaaagcccga aaatggcacc cgatgctccg
    23521 actgttggaa tgttaatcat aaggtttaat ttgcccatta aagtgtcggc atagttttgt
    23581 cccccctgca gcaggtcggc tccctgactt aataccacat caactgccac gggatccatt
    23641 ttgttaatta gcattgtaat ttcgatgtag tttaccagca gatgaaggaa agcggctccc
    23701 agaccggtaa cgaaataata aataaaaaat cgttttggtc cccagatgtc ttccagcacc
    23761 ctcccaaaca tccataatgc aaacatattg aaaaaaatat gtgctatact cccatgcatg
    23821 aacatatgag tgattaactg gtaaggttga aaatgcgagc tttttatact gtatattgcc
    23881 agataatcgt tcaggttgat acccgttcgt tgaagtatat aggtagcaag aaagaggatt
    23941 atatttataa tcaaaatatt tttaactact ggcgggaggc taaaaaaacc acccggactg
    24001 tattgattca tagcctataa ttagtttaat tttttttcta tctcatcaat ggcaacaatt
    24061 tccataattt tttttccgtc aggagtaaag ttgtgatttt tgcacaacat aagtcgaaag
    24121 aataaatctt tttgctctaa agaactcagt tgcttgggag aatgcaatga agttgtcttt
    24181 gcaattgaat acgccagttt ttcgttgtaa ctcagcttca gttgttgccc catgtcgtta
    24241 aacgtttgaa aaaattcctt aaaaatcagg catggattaa tatcggtaag tgctgaaggt
    24301 actccatgaa tagctattgt gcttttccca aggaaactta tgtcgaatcc gatattacaa
    24361 aggtcttcca ttacttcggt aagcaaagcc ctgtcggttg ctgataattc gatttgttca
    24421 gggaataggc ttttctgaca ggtggctttt ttgtcgacca tatgcgatag aaactcctca
    24481 tacagaattc gttcgtgcgc cctgccttga tcgataatca taagtcccga tttcacggga
    24541 gtaataatgt atttatcttt tatctgtaaa aactgaacat cttccgaaac tgaaacgctt
    24601 tcaatacctt tttctggttc gccctgaggc tgtttttttt cggtatcaaa cagcatttcc
    24661 caattggcag ggggtttacg cgtttctgca aaatttttat tgaacgaatc gtaatactct
    24721 ttttgccggt ttggtttatc gaaaggatta taatcgggat tgagctttat ttcgggaagt
    24781 ctgaattcgg tacgtgaagt aagatgaacg tcttttgtga tgtcttcttc aaatgcaaga
    24841 ggagcaacaa tattgaattt tcccagcgac tccttaacgg aagcattcaa aatttgaaaa
    24901 accgcttttt cttcatcaaa cttgatttcg gtttttgtag gatggatatt aatatcgatg
    24961 tgggaagggt caacctgcag gaaaatgaaa aacgaaggat atgtcgactg cattataata
    25021 ccttcgtatg gcaacataac tgctttccgg aaatatgggt gcagcataaa acgcttattt
    25081 acaaaaaagt actgctcatc gggcgttttt ctcgttttat cgggtttgcc aataaaacct
    25141 tctactgaaa caatactggt ttcgatgttt attggaataa ggtgttgatt gatagctttc
    25201 ccaaaaaccg aaacaatccg ttgccgtaag tttgaaggct caagttgtaa aatatccgtt
    25261 ccattgtgca ctagctttaa acggatatca caacagggaa tggcaatccg ctggaattct
    25321 tccacaatat gacggaactc tgcctgatcc gacttaagga acttgcgacg cgccggaaca
    25381 ttaaaaaaca agtttcgcac agctatgctg gttcccggcg aacagttgca caactcctga
    25441 gacatcagct ttgaagctga aatgtgtaat tctgatcccg tttcgtcttc ctgacgtttt
    25501 gttttaagct caacttcggc aacagcagcc actgaagcca ttgcttcacc acgaaatccc
    25561 atggttcgga ttgaaaacaa atcattggca tttctgattt ttgaagtggc gtggcgttcg
    25621 aaacacattc gggcatcagg agccgacatc ccgcaaccat tatcgataac ttgtatcagg
    25681 gttttcccgg aatcctttat tataacttgt atatcggttg ctccagcatc gagggcgttt
    25741 tccatcagct ccttcaccac agacgcaggc cgttgaatta cttcgccggc agcaatctga
    25801 ttggcaacgg catcaggcaa aagtctgata atatcaggca tgtttagtta tttataaaat
    25861 tcagaaatga agagaaatca acctgcatga gcaagtaaat taaaaccgca agaaatgcaa
    25921 gccggatgag aatgcgaaca gcagattgct tcttatattt cgaattcctc tgaaataacg
    25981 aactatagct ccccttaatg ctgctgcccg gattatcagg atcggttatg tcttcacctc
    26041 tttccagttt aagctgccgc cgccgtttat taatcctctc cttttcaagg tcaaaatacc
    26101 tgggttgata ttcaaatact gaatgaccgg gtaatttaaa aaaactgaat gccatacaaa
    26161 acaaatgttt atgcaaattt attaaaaata tctcaatttt atcgatttca cctgagcata
    26221 atcaatgata gctttgtaac gtattgattg tattttgatt gcatccatat cggaatatta
    26281 gtttaccgaa agccagcatt gtttttggcc caatgcttgc aaaaataatt tcgagttact
    26341 gagatttttt gcaactttgc aatcttataa aactgatatt catgaaagcg cttggtgtta
    26401 cattaattat ttttatcctt ctgaattctt atttgtttgc acagcataca acagagctat
    26461 ataaggtttt tggaagtccg tattcaagca ctattaattg catatgcacc gatatggatg
    26521 gaagcactta tgtgggaggg gaattcacca aaagtctgct tatgggtcca tataagttca
    26581 aggacaaatg gggaggtttc attgctaaag ttgatgacca gaatcaggtg atatggatga
    26641 agcgtgctga tatgaaattc cggaatatgg aattaatcga ctcatctctc attgtgtttg
    26701 cacaaaattc gcaaacagta aattttgccg gcactgaatt gcaatatagg ggacagaact
    26761 tcaactctgt tcttgcaagc atttcgatta atacaggcga tgttaagtgg attaaggaaa
    26821 tttccggaga gggagatgta tatgcggctg atatgacaat atgcaaaaaa gacatattta
    26881 ttactggctc atttcgtgtt ggaatggcac tcgataaata cacacttaag cagacccacg
    26941 aaaaaaataa tttcattgca aggcttacag ccgaaggtaa agtaatatgg ttgaacaaag
    27001 tttcaggggg tgatagtttt attaccggtg caaccatctc cgctattgca gcaaaccaaa
    27061 acggcgactt tgtaatttca ggtgaaattg ccgggtcggc tgattttgcc ggctattgct
    27121 tcgatgtagc tgaatttaat tcaaaatcgg aaaacacaac ttcagaaggt tgcgtatatt
    27181 atgcttattt tacgggagat gggaaatgca ttcgggtgaa taaggcaatt actgaggctg
    27241 aagttacgga tatcgcttta ttcggcgacc aggtctatct ttgtggttat tactccggag
    27301 cctatttgtt acagcgcccg atagctgctt caattttcgg agatacagtg gtgttaaatt
    27361 ctgtgcaaag cccaacaggt agtttgctgg aaacatggtt tgtgatttcg cttcgcgata
    27421 atgagccatt atgggcttta actaccaaag ggaaaaattc gagccgagcc ttgagcctgg
    27481 ttgtggacga tcagggtagc tgttatgcgg gtggttattt ctacgatcat attattttac
    27541 ccgatggctc ggaagtaaat gcgatcaatg ctgagttcag cgctggggct ttggtcatga
    27601 aaattgacag aagcggaaaa ttgctttgga cacataccgc cagctcacct acagggatta
    27661 ataaattctt cgatgtgtcg ctcaatcata gccttgttgt ttgtggcgaa atcagcggta
    27721 gtgcagtttt cgataagtca ttgatccagt cgcgaggcaa acaccagaat ggatttactt
    27781 ataaatttga tttataaaaa caggtagtat tgaattccct gaacgatttt attcatattc
    27841 ttgaagctga aaaccagctt ataagagtgc agtccccgat aagccctaat ctgcaaatta
    27901 ccgaaattgc cgacagaata atgaaatcgc cagatggtgg taaggctatc ctgtttgaaa
    27961 atacaggaac tgcatttaag gtgttgataa acgcttttgg ctcagaaaaa aggatgggca
    28021 tggctttggg ttacgaaaat gccgatcaag ctgcatttgc attggcttcg gtactgcaga
    28081 ttcttacaag agagaaagaa tcattttgga ataagttgaa aatggtacct gtattaacga
    28141 aaattgcttc tgcaattcca acacttataa aaaagcgggg aaaatgtcag gaggtaatac
    28201 aaactaaccc cgacctggac atattaccca tattaaaatg ctggcccttc gatggcggca
    28261 agttcataac actcccaata gtaatcacaa aacaccctga aacaggtgtt cgcaattcag
    28321 ggatgtatcg tatgcaagtg ctgagtaaaa ataccacagg gatgcactgg cacttgcaca
    28381 aaggtggcgc ggcgcattac gaagtgtata aaaaaatgaa aatgagaatg ccggttgcgg
    28441 tggctcttgg cggggacccc gtttacacct atgctgcagt tgctccggcg cctgacgata
    28501 tagatgaatt tatgcttgca ggccttttgc gaaaacgcaa ggtgaaattt gtttcctgta
    28561 tcacacagaa tattgaagtt cctgccgatt ccgatatagt tatagaaggc tacatcgata
    28621 cagaggagga tttttgcaac gagggccctt ttggcgatca tacaggaatg tattcgttac
    28681 ccgatgcata tcctgttttt catgttaccg ctattacgca ccgaaaagac gccatatacc
    28741 cggctaccat tgtcggtgtt ccacctatgg aagacaggta ctttgcgctg gcaaccgaaa
    28801 aattattttt cccggcactt aactttcttt acaaagaaat tgtgggtttt cacctcccat
    28861 ggtttggtgt tgcgcataat cttgtattgg taaaaattag aaaacgatac cctggtcatg
    28921 catttaaaat catgcatgga ttgctcggta acggacaaat gatgttctcg aaaaccatta
    28981 tctgtgttga tgaagatttg gattttaata aacccgaaac ttggttaaat cgaatcctgc
    29041 agaacattca gccggcaaag gatgttgaaa ccggctcagg ccccacagat gtgctcgatc
    29101 atgctgccta caggtttgca tatagcggga aattgctcat tgatgcatcg aatttggtgg
    29161 aatcggatat ggaaaatcca gtacatacaa aaaaccctga tttacctaac tattctgttg
    29221 tttcgctggg aacttgtctt ctggcatttc ctcaggccaa tacaaaacca gatctcgccg
    29281 atttcgccga aagactatct caccaaacag cttcaggtga atacaaagta gttgtaatta
    29341 ccgagaaggc cataatagat ctgtcgcctg agctgaaagc atggtacata ttgaacaact
    29401 ttgaccccgg ccatgatagc ctggttgtaa ataactgctt gtttgtgaat gctaccataa
    29461 aaacaactgc gaaggatgga ttcagcagaa actggcccaa tattgtaagc atggacgaca
    29521 aaacaatagc cgatattgat gctttgtggc cactgatttt ccctgatagt ccaatgattt
    29581 caccttcgtt acaatataaa aaactgattg tgaacaaagg ggcttcaatt acaagcaagt
    29641 aacgatatga gcaattcgta cgaatccgtc ttaaatcaca ttgaattaca cgggtatgaa
    29701 ttcagatttg tagagtattt caaaaacgga ttcaggttat ttaaaaaata cccatttggt
    29761 tttttaagct acggaattaa tatttcattg gtaatatatt tttcgggttt tttgggcatc
    29821 cctgggagta taatatcgaa cctgctgcag ccggtttttt tggcaggtat ttatattgtg
    29881 gccaatgatt tgttacgtga gcagacaccc ggatatacac gattttttag tggatttgag
    29941 caatttgcaa aattgtttgt cgcgcaaatt gttacgttct ccttggtatt ccttggtttg
    30001 ttgttactcg ttttaccggg aatttatctg gcagtttgtt attcctttac ggtatttttt
    30061 attgttttca tgaaatacga ttaccgaaaa gctatggatt ggagccgaaa aattgtaaga
    30121 atcaattttt ggccggtact tggatttatg ctcgttatag gcctgattaa tgttttggga
    30181 gcattggctt ttggaatagg attggccttc actttgcctt ttaccgcctg cgtaatgttt
    30241 tcggcattcg aggatgttat ttcaactgca gcagagaaaa cgggcggagt gggagaatac
    30301 tgattgatat tatctgatgc tgagtaattc agcaattttt tcgggatcgg ttcgtaatcc
    30361 ttcaacaaca taggttgctt tgcaataaaa tgcttctctc ttcaacaaag cttgtttaac
    30421 aaaaaactca agctcatcat cattttttcc gaatattaag ggcctgtttt tttttgattt
    30481 ttttattcgc tgaataaggc tgatggcagg gagtttaatg tatatgctga taccgtttcc
    30541 gttcattaca tcaatgtttt caccatagca gggggtcccg ccaccagttg ccatgacaaa
    30601 atttttacac gcagcaaatt gttttagtaa tatcgattcg agcctgcgga actcttcttc
    30661 tccctgattc ttaaaaatat caggaattgt cgtttttaca gttttttcaa tttcttcatc
    30721 aagatcgaca aaagtgtaat gaagcctttt tgccaatttt ttgccaatgg ttgttttgcc
    30781 acaacccata aatcctatta aataaattct ggtatcgttg ttattcaaag tcaaggctca
    30841 tttgtttggg gtcgctttca ggctctttgt ctttgcttga atctgatttt gcaggctttg
    30901 tggatggctt tgcttttttc ggttttggca aatcacgtgt aacagtctct tcttcaattg
    30961 aagtggtgtc aattatttca aattctatct catcaggagt ttcatcctga tcgaattcgt
    31021 caggttcgat gtcagaatca aggttttcag caggaatttc tttctgaaga ggttcaataa
    31081 aatctgtttt ctcaacttcg taattagaga ttcgtttccc ccgggcttta atacctttta
    31141 cagctataaa attgtctacg tcaataattt ccttcggacg cgaagcttgt tttcccccga
    31201 aaattatttc aacctgtggg aagcgatcat cgcttagcat tattagtttt gtgccttcat
    31261 cttccggaat gtagaactgg tctttggtgg tatcttcaaa cagaaatcgt tttatatagt
    31321 aaaatccttg cggtgcgtca taatagagcg ctgtccatat tttatcggga ttatattttt
    31381 caataagcag tattgcttca tcgtagtggt tcgacaagtc gaaattggta agacaatatg
    31441 tgcctttttt gtaaacgact ataattttat catttccatg aaactctccc agataaatgc
    31501 cccgcccatc tgcattgagt cggagaactt cgtcgtcgaa ccagattttg cgaccgccca
    31561 gcgttgaaac cccttcttct ttcaaaacaa ttttatgtat gtcgtttttc gtaatgatgt
    31621 ttcctagtga atttcggcct ttaattgcaa tttctttaaa atcggcttca aaaatcagct
    31681 tacggatttt aggtttgggt tttagatata ttttaattat ctcagcttct ccattaggat
    31741 tacttgtgaa atataccagt ttcgacccca attcgccttt tgtaatatcg tactctttat
    31801 cgcggttaac gccggtaacg gcaaaacgtt taatgaagta attcccgaac ctaccatccc
    31861 tgtaaaccac attgtatatt gtgcgggtat cgtttttccg gaacacatct aagtgaatga
    31921 tgtctttgcc aaaaaacgct ttttcactaa ctttggtaac aatatactta ccatcacgtt
    31981 taaatacaat aatgtcgtcg atgtcggagc attcgcaaac aaactcctct tttttcattc
    32041 cggtacccac aaacccctct ttacggttta catataactt ttcgtttgca acagcaacat
    32101 tcgaagtatt tataatgtcg aagtttctga tctctgtttt tctctctcga cccttcccgt
    32161 actttgcttt tatgtgggtg taataattta ttgcataaac agtaaggtgt tcaaggttat
    32221 gttttacctc ctcaatctgc ccttcaatag aagtcatttc ctcttcggct ttttcggcat
    32281 tatacctgat tatgcgcttc attttaattt cgagcaatct cagtaaatca tcgttggtga
    32341 tttcacgcct gaaaagttgc tgcgatgccc gggcttttgt gccaataaat tctatggcct
    32401 catcgttggt ttgtgagttt tcgtatgctt tgtctttata gattcttttt tcgatgaacc
    32461 atttttccaa tgatagcata tgccattgtt cctcaagctc ccgcatgcga atgagcagtt
    32521 cttgttcgag caacttaaca gtgttatata ccgaagcctg aagtatatcg cttactccca
    32581 taaaacgggg cttgtcttcc tcaattacac atgaattcgg agaaattgaa acctcgcatt
    32641 ttgtgaatgc atacaatgca tcaatggttt tgtcgctcga cactgcggga ggaagttgta
    32701 taactatttc cacattcgac gaagtgttgt cgtcgatctt cttgattttg attttttctt
    32761 tttcgttggc aagtaaaata ctgtcgataa cagagtttgt ggtttcgcca aaaggaatgt
    32821 cggtaattac cagcgttttt ttatcaagct tctctatgtg tgctcgcact ctcactttcc
    32881 cacccctgat tccatcgtta tatttcgaaa catcgatcat tcctccggta aggaaatcgg
    32941 ggtacagttc aaagctttca ttttttaatg ttgcaataca tgcatcaata agctcgttga
    33001 aattgtgggg aagtattttc gatgccagcc ctacagctat cccttcaaca ccttgtgcaa
    33061 gcagaagcgg aaattttacc ggcagagtaa tgggttcttt attgcggcca tcgtacgagt
    33121 tcttccagaa agtggttttg gggttgaaga caacttccag tgcaaatttc gaaaggcgcg
    33181 cctcaatata cctgggagca gcggcagcat ctccggtaag tacgtttccc cagttcccct
    33241 gagtatcgac tagcaagtct ttctggccca gttgcaccaa agcatcccct atggatgcgt
    33301 cgccatgggg atgatatttc atagtatgcc cgatgatatt ggctactttg ttgtaacgac
    33361 catcatcgag gtctttcatt gcatgcaaaa tgcgccgttg tacgggcttc aacccatcgt
    33421 aaacatgcgg tacagcgcgt tccagtatta cataagaagc ataatcgaga aaccaatcct
    33481 gatacatgcc cgtaagcctg gtgtactgaa tatcggcaac atgctcctcg ataccatttt
    33541 cgctggtttc ttcaacatca tcaaaccccg atatttcctg gtcttcgctc atctttttat
    33601 tagcatgttt ttagcaatca gcgtttatct aaacaatggc atcttcttct attctcaggt
    33661 tttcgataat aaacagctgc cgttgattgg tgttttttcc catatagaac tcaagcagat
    33721 cgtttatcat atctgcttta ctcaggttaa ccggatcgag gcgaatgttg ttattaataa
    33781 aattcttgaa ctcatcggga gaaatctcac ccaggccttt gaatcgtgtt atttcagggt
    33841 tgcctttaag tttggcaata gccctttgct tctcactttc atcgtaacaa tacagcgttt
    33901 cctgcttatt gcgaacacgg aacagtggtg tctgtagaat gaacagatga ccattacgta
    33961 taacttcagg gaaaaactga aggaaaaagg ttatgagcag taacctgata tgcataccat
    34021 cgacatcagc atcagtagca ataactacat tgcggtatcg cagattttcg attccgtctt
    34081 ctatattgag tgccgcttgc aacaagttaa attcttcgtt ttcataaaca atttttttac
    34141 tcagtccaaa agtgttcaga ggtttgccct ttaaactaaa aacagcctga gtgttaacgt
    34201 ctctcgattt agttattgac ccgctggctg aatcaccttc agtaataaat attgttgatt
    34261 caaagcggcg ttcttcatcg gtattgtagt gaacacgaca atcacggagt tttttattat
    34321 gaaggttaac tttttttgaa cgttctctcg atatcttttt aatatcctga atggcttttc
    34381 tctctttttc cgaagcaatt attttccgaa gaatggcgtc ggcagtttcc ggatttttgt
    34441 gaagataatt gtcaagctct ttttttatga agtcgttgac aaatttatta atacttattc
    34501 cgtcgggagc catgtcgcgc gatcccagtt ttgttttggt ctgcgattca aaaattggat
    34561 cttccacttt gatgctgatg gctgcaacaa tcgactgccg tatatcggag ctgtcaaaat
    34621 ctttccggaa gaattcctta atagtttttg cataggcttc acgaaaagcc gaaaggtgcg
    34681 ttcctccctg agtggtatgc tgcccattta caaaactgaa atattcttca ctatatttgt
    34741 ttgaatgagt aaatgccagt tcaatatctt ctcctttcag gcaaacaata gggtaaaggc
    34801 actcactgtt catgttgtcg ttcaggagat cgactagccc attccgcgaa aagaatcgtt
    34861 gtccgttgag gtatattgat aatccggtat tcaggaatac atagttcttc agcatgttct
    34921 cgatggtctc ggtcctgaaa cgaaacgaag gaaacagctc aatgtcgggt gagaaaacaa
    34981 tcagtgttcc tgcgggttca tcagttttct caataggcgc atcgtttata agaatgcctc
    35041 ttgaatatac cgcttctttt ttttctcctt ctctgtaagc gaccactctg aaatcggaag
    35101 aaagtgcatt cacggcttta atacctactc cgttcaaccc gacggctttt ttgaaaacct
    35161 ttgaatcgta ttttgccccg gtattcattt tcgacacaac gtcgattact ttgcccagcg
    35221 gtattcccct gccatagtca cgaacacgta ctgttgattc ctcaacttca atatcgattt
    35281 tgcttccaaa ccccattgca tattcatcaa ccgaattgtc aatgatttct tttaataaaa
    35341 catagatacc atcatcgcgc gatgacccgt ctcccagttt tccgatatac attccaggac
    35401 gctttcgaat atgttcgcgc cagtcaagtg tttttatcgt ttcttccgaa tagtttgcta
    35461 ccatgtacac acattgtttc acaagaacaa caaaaataat aattattaat tgtattcatt
    35521 gcagcgcaat gaatagatat taacaaaaaa aggttaataa tatttattgc cagtaacttg
    35581 ttcggacgaa ccttttcaaa aagctaaatg tctttatttt ttataatttt gccgcaactt
    35641 acactatgct tcaaggcctt gtaataaaat cgtttggtaa aacccattat gtgaaaacat
    35701 cacttgggat tctgaagtgt agtctaaaag gagctttgcg cattaaggga taccggtcgt
    35761 cgaacctggt ggcagttggg gatagggtgt cagtagagca ggaaaaccag ggagagggtt
    35821 ccattgcaga aatctttgac cggaaaaatt acattatcag gcgttctacc aatctttcga
    35881 gagaggtgca aataattgcg gccaacatcg atttggccat attgatggta accctgaaag
    35941 agcccaccac ttcaacagtt tttatcgaca gatttctggt ttcggctgag gcttatcaaa
    36001 ttcctgtgtt gctcgttttc aacaaaattg attcttgcaa gggtaacgat atcgaaaaac
    36061 tggccgaaat gatggctgca tacgatatgg ccggctatca acttgtatct gtttctgtta
    36121 caaaaaggat aaatattgat aaaattcgag aagcagttga gggtaaaacc agcgttttgt
    36181 gcggcctaag cggagtaggg aaatcatctc tcattaatgt ccttgatcca tcgttctgtc
    36241 tgcccgtttc tgatatttcg gaaacccacc tttcggggaa gcatactaca tcctttgcac
    36301 aaatgctcga actgaacttc ggtgcctata tcatcgatac accgggaatt cgtggttttg
    36361 gtatcgttga cataaaaaaa gatgaactgt accatttttt tcctgagatt tttaaatttt
    36421 ctaaatcctg tcagttttat aattgccttc atctcaacga acccggctgt gctgtgctga
    36481 gtgctgtggc tgatggcaat attgccgaat cgcgctatcg gtcgtatgta agtatgtatg
    36541 aagatgaaaa taagaaattc agaaaaaaca aatttctgta aaacaaagct tgtttttact
    36601 gcgcagagct atcaccggaa aatgtattta ttggcttcag gttttgaaaa tcagttcaaa
    36661 ataattattt tcgggaatta cgaaaaagaa tgaagcaacc caaaaatatt gttgttctaa
    36721 ttatgctttg gccttttgca gtcttataca ggtttattac ggcattgcgc aattttatgt
    36781 acgacaataa tattcttccc tcggtggaat tcgacattcc ggttatatcg attgggaata
    36841 tatcggtagg cggaaccgga aaaactcctc acaccgaatt tatcatcggg atgctcaggg
    36901 aaaaataccg tattgccgtg ctgagcaggg gctataaaag aaaaacaagc ggatatgttg
    36961 aagtttccga gacctcgtcg tacttacaag ttggtgacga accgctgcaa atgaaacgca
    37021 aatatccgga aaccgttgtg gctgtttgcg aaaaaagggt aaaaggcatt aaaatattaa
    37081 tggagaaata tccagatttg aaccttattg tgctcgacga tgcatttcag caccgaagag
    37141 taaggccggg cctttcggta ttgctgaata acttttacca tccgctttca tcagaccatc
    37201 tgctgcctta tggcagactg cgcgaaagcc cttcgggagt actcagggct catgtgatta
    37261 ttgtaacaaa atgccccgaa aacatgaaag ccattgaaag gcgtatcatg cttaaagaaa
    37321 tggacccaca accctaccag tacctgtttt ttacccgctt cggatatcat ccaccccgga
    37381 ctgttttcag cacaccgatt gaattggata ttaccgattt aaaaaagtat catgtcctgg
    37441 cagtgtcagg gattgctttt gccaaagatt ttatcgatta tctcaactca acatttgcat
    37501 ccgttgatca cgtcgagttt gccgatcatc atgatttccg caaacgggat atcgaaaaaa
    37561 tcgaagcgcg attcaatgca attgcagggt ataagctgat tgttacaacc gagaaagata
    37621 gcatcaggct ccttgagtgc ggcttcatgc ccgatgcctt gaagcgatac ttcttttata
    37681 ttccgatttc tgtaattttg caaggtagcg aagaagataa aaaacaattc gataatcaat
    37741 tttttacata tgtttcaaac aataaaagat acagcagcct ttataaaggc acaaatctca
    37801 ggtaatcctg aagttggcat tgtgcttggt accggacttg gtgggctcgt ttcggagata
    37861 gagattgaaa aaatcatttc ttacaatgaa attccgaatt ttccggtttc aactgtcgaa
    37921 ggtcattctg ggcagttgat ttttggaaaa ctttccggaa aatttgtgat tgctatgaaa
    37981 ggcagatttc attactacga agggtatgct ttatcacagg ttgtattgcc aatacgggtt
    38041 atggctcagc tgggtattaa gaatttgttt ctcagtaacg ccagtggggg agttaacccg
    38101 gattttgaaa tcggagatct gatggttatt aacgaccaca ttaatttgat tcccaatccg
    38161 ttgataggga aacatgaacc agaattcggt gtgcgatttc ccgatatggg acaggcttat
    38221 aatcccacac ttattcatcg cgccggtgag gtggcaaaag cacttggaat aaagcttatg
    38281 tatggagtat atactgctac aaccggccct acttacgaaa cgccggcaga atacaggtat
    38341 ttcagaatta ttggttccga tgctgtgggt atgtcaacgg ttccagaggt tattgttgcc
    38401 aatcaaatgg gaattccttg ctttgccatg tcaatcatta ccgatctcgg tgtacctggt
    38461 aaaatagtta caattactca tgaagatgtt caaaaggtag ctgccggtgc agagaaaaag
    38521 atgacacaga tttttaaaaa actaattgcc gatttataaa ctggggtaaa aaaaagctct
    38581 caggcgagag ctataaaaca aagttgtttt ttattgtgta ggcataagaa aggccattaa
    38641 ccacacttgg aatatccgca actccggcaa attacacagc catcctgata aacaagagtg
    38701 tctttatcgc cgcagcccgg acattcaact cctgcttccg aggcgcgtgt gccattggga
    38761 atgtacttgc gcaaagcgcg ttctacccca tttttccaag tgttgatgct gtcgcaatcg
    38821 agctcaagag agccaacaag ttcaacaact tttggaatgg gcattccgta acggagcaca
    38881 ctgctgatca gttttgcata gttccagtag tttttatcaa actggtgcga caagccctca
    38941 aaagttgttg agtaattgta cttgtttttg tactgaaaat cgtagcgcga tgttccatct
    39001 tcgaaacgta ttttaacaat ttgccctttt tcgattgatg aaggtatgtt tagcacgtct
    39061 tcttctaccc tgcccgtaaa tatttcgtaa ggacagccat caagcaaacc aatgaatgca
    39121 atccattttt ctttattgtt ttggaaacgt actatatcgg catcgagtac ctttggacgt
    39181 ttcagagggc cttcttgccg tactactttt tcttttttct ttccggcaat aagaattccg
    39241 tctctcgatc cttcgcggta aaccgtaata cccttacatc ccgatttcca tgcctcaagg
    39301 tagcattcag caacggtttc ttctgtaacg ttttcaggca tattaatagt aacgctgatg
    39361 ctatgatcca cccatttttg aatgtcgccc tgcatacgaa ccttattcag atagttaaca
    39421 tcggctgcag tggctttatg gtaaggcgat tttgcaacaa tttcgttaat gtgtttttcg
    39481 tcgtattctt tcacttcatt gaagtcgtaa ccatttactc ttagccaggt gatgaattta
    39541 tgatggaata cgttatactc ttcccattta tcaccctgct catcggtata gtcgatacgt
    39601 gcattggggt cgttgggatt tactttgcgg cgacgacgat aggcggcacg aaaaaccggt
    39661 tcaatacccg atgtggtttg tgtcattaaa ctggtagtcc ccgtaggtgc aatggtaagc
    39721 agcgatatgt tgcgtctgcc gcaggtttcc atcgtttcta ccatttcggg gtcggcttga
    39781 cgaagccgat tgataaatgg attcgtcatt tcccgttgtg ggtcgtagat tgcaaagcat
    39841 ccacgctctt ccgccatttt tacagaagag cgataggctt caacagccag aattttctgt
    39901 acctcaacgg caaaatcatt tgcttctgat gaaccgtacc gcagattaag tgcagccagc
    39961 atgtctcctt cggctgtaat ccccagacct gtacggcgac cttccgaagc tttatgcctg
    40021 atattatccc aaagcttacg ctcaacacgc ttgatttcga aatcttccgg atcgttgttg
    40081 attttttcga tgatactgtc aattttttcc agttcgaggt cgatgatatc atccattaga
    40141 cgttgtgctt tttgcacatg atcgcgaaac ttatcgaagt tgaagcgtgc ttcggtggag
    40201 aaaacatttt ctacatagct gtaaagattg atagcaacca ggcggcagct gtcgtaagga
    40261 cacaagggta tttctccgca cgggtttgta gaaactgttc gatacccctt gtcgccataa
    40321 caatcggcaa ccgattcacg ggttattgta tcccagaata aaatccccgg ttctgccgat
    40381 ttccatgcgt tggcaataat tttatcccac agtttttttg cattgatttc tttttgaaac
    40441 ttgggattgg gtttgtcaat cggaaactgc tggatataag gtttgtcatc gacaacggct
    40501 ttcatgaagt cgtcgtgtat ctttaccgat acattggctc cggtaacttt gcccggtgtc
    40561 attttggcat ctataaagtc ttcggcatca gggtgtttca ccgaaatgct gagcataagt
    40621 gcgcctcttc tgccgtcttg agcaacttcg cgggtggtgt ttgaatatcg ttccataaag
    40681 ggaacgattc cggtggaagt taaggcagag ttcttcacag gggttccttt ggggcgcaaa
    40741 tgcgataggt cgtgtcctac gccacctctt cgcttcatta actgaacctg ttcctgatca
    40801 acttttagga ttgctccgta tgaatcggcg tctttgtcat ttccgattac aaaacaattc
    40861 gaaagcgaaa caatttgcat attgtttcct attcctgaca tcgggcctcc ctgagggaca
    40921 acatacttga aatccttgag aagctgataa atctcttctt cgcctaacgg atttttgtac
    40981 ttcatctcga tgcgatgcaa ttcccgtgca agacgacggt gcatgtcgtc gggtgtaagt
    41041 tcatacaaat tgccgtccga atccttcagc gcgtacttgt taatccacac tcgggcagcc
    41101 agatcgtctc ctttgaaata atcgagagtg gctaaaaatg cttcttcgtc ggtgtaagtt
    41161 cttaggtttt cagtcaccgt ttttgtctca gtttgtggtt ctaccaatag cataaaggtt
    41221 aatattttta aaagttctta aatctttgat atggttgcgt gtttttgcca cactaaaata
    41281 aataatgaat aaaatcgata acatttgttt ttttaacatc aatattaaca taaattgttg
    41341 ctgggaataa ctatcagaaa atcaagaaga tagtaaaaca caagctttac gaaaaaaaaa
    41401 gaaattaacc tctaaattac ttgtaaatgc ttgaaaatag gcactattca caagcaaatt
    41461 ctaatttata aacaattatc tgtttaatta ttttagacaa acctattgac aatgcaaatg
    41521 tttgaaattc agatggttga atgatttggt gagaataatt ttcaacaatc attttgggat
    41581 aaaaaaaaca aaaaataggc taaaaacatc atttaaaaca aaaaaccagt tattttatca
    41641 taaatccggt cgacagaacc cttatttgaa aaaacaaaat ctgcagcaat ttttccgggg
    41701 atttctgcag cgtttttatt tagtaaaaat ggagcaatcc cattagtaag gtctgttgaa
    41761 ttatgaacgc agaatgccgc accgcaatga atcaaaccaa cagcttcatt aaactttctg
    41821 aatgccgggc cgaaaaaaac aggcacacca taagcggcag cttcaatcgt attgtgaata
    41881 ccctttccga aacctccacc gatgtatgca atatctgcgt agcggtacaa tcgtgaaagc
    41941 agtcccattt tgtcgattac cattacatct gcattgccta aggcggcatt atctgtttca
    42001 gagtaaacaa ggcaattttt tccccaaagc gataatatat catctatatg tttacggtgt
    42061 atttcgtgtg gcacaacaat gagctttaac ctaccggcat agctccggaa agtctgcgat
    42121 aatattactt catcggcagg ccatgtgctg ccggcaatca aacacatgga attgcctttg
    42181 aagatttcaa ttaaagtatt tgaatagggt tccgatacaa tctgtattac cctgtcgaaa
    42241 cgggtatcgc cttccacgga acaattgctg cactcaaaat tttgaagaag ctgtaaagat
    42301 tgattgtcct gaacaaatat ccaatcaaat gttagaagaa ttttttttgc aaaaaaggtt
    42361 ttaaaacgta atagccaatg cttctttcgg aaaattgcgg aaataagaaa tactttagtt
    42421 ttactttcag cggctttgtt gatgaaataa taccaaagtt cgtacttaac aagaattaag
    42481 gcttccgggc gaatgatgct aaagaattca gcagcatttt ttctggtatc gaacggaaga
    42541 taacaaacat aatcggcata agcataattt ttgcgcattt cgtaacccga gggtgagaaa
    42601 aagctcaaca atattttctt atggggatgc atttgtttta ttttttcgat aagcggcctt
    42661 ccctgttcaa attctccgag cgatgcacaa tgaaaccata tccaggaatg ttgtgtgtcg
    42721 gtgtttttcc gaagcagtgc ctcccagttt ttacggcctt ttatcattag ctttgctttc
    42781 ggattgaata aagcagcaat tctaacagcc agaaaataca gtcctgtaaa tatccggtaa
    42841 acaatcagca tactttaatt aataatagta aaactcatta gggtttcgat aatacagggg
    42901 taaaatccag ccagccctta acgaatacaa ataatcgtga tacagtacat cgtcacgact
    42961 catggtatcg aaattaaaat cgcgtcttga tttagtccat gcctgataaa actctattcc
    43021 aacatagaaa ttaaaatgcg atttattatc gaggtactgg tagcccagaa atgttttggc
    43081 ggcaaatccg ttgcaaagtc tgtcgtatcc ttttttgtaa tcgcccagaa tgcttggaac
    43141 attattatct ttattctcga ttcggatatg gtgctgcagt aagccgcctc ccatctgaaa
    43201 aataatcccg gaattgctgt tgggcttacc tatgggaaaa agttttccgg ctttcagtcc
    43261 ggcataaaac cccgtttcgg taaacattac cgtggcgtat tcgccatact catttattat
    43321 ctgccggttt gaggttcgga gtgcatcgag tattgtggta tctcttttaa tttttgtccc
    43381 gaacagatag cttatttcac caccaaacat aaaattgttt cgggttttgt aagttagacc
    43441 tgcaccaatg gttgaattta cgccatattt ctttgcaagg tctccaccgg gaacctgaaa
    43501 cgaataattt atctcaacct gccagatgtt aatcgtggaa tcctttatct gagcaaataa
    43561 cgggatactt actaaaagaa aaatactggc tgtcagaaat cgttgcatag gcataatgga
    43621 cgggtcaaaa atactatttt ttcaatattg tcgggagcaa gtggttttaa tatttcagaa
    43681 tatgctaaaa tcaaattaat ttcgttctat ttcgcaaatg ttgtactttt atcttaaact
    43741 catacaagct gaagttatta tttctgatag cggtttttat tcttttttgc acacacaatg
    43801 tagtggcaca ggaaagtatt aaccttaaaa aaacacagtg tattattagc gatacggttc
    43861 agctcgacag cttgagtatt gttgatggaa gcgttgtgtt gtatgatgaa aataataata
    43921 ttattcctga tagctgctat agaatcgaat acgctgaagc cagaatcatt ccgcataaaa
    43981 gtattgtcgg aaaacaggta acggttgttt atcgtagttt ttcgttttca tttgttaaac
    44041 cctacagtca taaaaaacgc gagcactttc tgagcagttc tttactcggg gacgaagaaa
    44101 ccgatatttt tagttatcaa tcgaatgctg acggtttttt taatagcact gagctgaacc
    44161 gaaaggggag tattgcacgg ggtataagtt ttggcaacaa tcaggatgca atactgaact
    44221 caaacctgaa tcttcaattg tcaggaaaac tcagcgatga tatcgaaata ctcgccgcaa
    44281 tttccgataa caatatcccg attcaacccg atggaaatgc acaacaacta caggaattcg
    44341 ataaggtatt cataactgtt ttcagcaaaa ggtttagcct gacagcaggc gattttgaag
    44401 aaaaaaagcc acagggatat ttcctgaact ataacaagaa agttcagggc attaaatttt
    44461 cattgatcga ctacaatatt tcgaagaata tcactattac ttcaagcctt ggaggttcgg
    44521 tggcgaaagg aaaataccgg cgacaaacaa ttcagcagat cgagggtatt cagggcccgt
    44581 ataagttgac cggagcaaac aacgagacct atattattat tcttgcaggt accgaaaaag
    44641 tatatatcaa cggtgagttg atgcagcggg gccaggaaaa cgactatacc atcgattaca
    44701 ataccggtga gctgacattc actgccaaac ttccggttac caaagataca cgcatcattg
    44761 ccgagttcga atattccgaa agaagctata cgcgtttttt aataacaagt aatcataatt
    44821 tacaaacctc aaaaggaaac ttctggttca atgtgtactc agaacaagat aataaaaacc
    44881 agccctacga ccaaaatcta acctattccg aaaaactgct tctttcacag attggcgata
    44941 acctgtcgga tgctgttgtt cccaattttg acagcgttgg attcagtgcc acggaaatac
    45001 gatacagaat ggttgatact attgtcggtg ctgttcagta cgattcggtg tttgtgtatt
    45061 ccaacgatga aagtctggcc gtatatcgac tgggtttctc gtatgtgggg gaggcaaacg
    45121 gcaattatat aaaaagcatt tcttcagcca acggcagggt attccagtgg gttgcaccca
    45181 taaacggagt gatgcagggt aattacgaac cggtgatact actggttact ccgaagaaaa
    45241 aacaagcatt cagccttgga gggaaatata gactttcgaa atcaacagtg ttatcagccg
    45301 aaacagccat cagtattaac gatcagaatc tgttttccaa atacgataaa tcggatgata
    45361 ctggctatgc ggcaaagttc caactcagtc aacgaatttt ttcaactact tccgacagcc
    45421 tgttttttag tgccagatac cagtttgccg ataaaaattt tgatgccgtg gaaaactacc
    45481 gtgaagcgga gtttgcgcgc gactggaacc tgaacgatgc aaatgccctg cactttgaac
    45541 aattggcagg aattaatctg aactatgccc ataaaaataa agcacacgca gcctacagtt
    45601 tcgaccttct gaatcggccc ggaatatatg ctgggtacaa caataaagct tttggcggaa
    45661 tcgataccgg aagatggcag tttaatgctg aggcttcgtt tctgaaaacc tcggatacta
    45721 tttttaccac atccttttta cgtcaaaagg catctgtatc acgaaatttc aaattcttta
    45781 aaattggagc aggggaagaa caggagtaca atgcatggag atataaccaa accgattctc
    45841 ttgcaggtaa cagtttccgt tggtttcagt ataggttttt tatgcagact cccgattcgt
    45901 cgaaaaacgg atttctttta gcgtatcgca acagaaccga tgctttgccc tacttaggca
    45961 actttagtaa tagcacaggt tcacacgatt tcgaccttga actgaaattg ctgaaaagct
    46021 atagtcaatc gttgagcgtt ttaagtaatt tcagaatgct gaatatttat aacgattcgg
    46081 ttacgcaggc agaaccccag gataacctca ccggcagggt tgagtacagt tttaaaattc
    46141 tgaaaggagc tgtaagctcg gcaactttct atgaaatcgg atctgccatg gaacggaaaa
    46201 cagagttttc gtatttagaa gttgctccgg gtcagggagt atatatgtgg actgattata
    46261 ataataatgg tgtgcaggaa cttgatgaat ttgaagttgc ctatttccag gatcaggcca
    46321 attatatcag gtattttaca acaaccaccg attatattaa aacctatacc aatcagttta
    46381 gccagatgtt aaatataagg cctgcccaga aatggaaaaa ccttaccggt tttgccggtt
    46441 ttatgacccg tttttccgat cagttcgcat ataaaataag cagtaaaaac accgcagacc
    46501 aggtagcaac ctacgcaaac ccgtttgcaa acaatgtaag cgacacttct cttgtgtcgt
    46561 taaataacac catccgtaac gagttttcgt ttaataaggg caaccccaaa ttcggatttg
    46621 attatatttt tctgaacaat aggaataagt tgctgctggt aaatggtttc gacagtcgta
    46681 ttttgttgag gcacgaagtt atgcttcggt tttccataag aaataccttt cagttcaata
    46741 acctgattgc catgggtaat aaaggatacg attctcagta ttttgtaagc agaaactatg
    46801 atataagatt tctcgaaaac acggcgtcgg tatattttca gcccaatatt caaaaccgaa
    46861 tagagcttaa gtacaaatac aaaaacaaat cgaatcttac cggagcagaa tctcttgtaa
    46921 gccatgatgc aggctttgaa taccgattga gttcggtaaa gcgtggggca ttgagtttca
    46981 atgtcaatta cattctcatg ctgttcgatg gcagcagcaa cagttcggta ggttacgaaa
    47041 tgcttgaggg cttattgccg ggaaacaacc tcacatggca gctgagtttt cagcatcagc
    47101 tcgccaatgg tttgcagata aacctcagtt ataacggtcg tgctgccgaa gatgctaaaa
    47161 cagtacacac agggggagtt cagttacggg cattttttta gcaattgagc gtattctcct
    47221 ttcagcactc ttagtttccg gggaagatta tcgtaaacca actgatacga taggtcgatg
    47281 agttccttaa ccagcttttc aggagcaccc tgttccatgt atattgtatt ccaatgcgtt
    47341 ttattcatgt gaaatgccgg aacaatagag tcgaaacgtt ccctcagctc ttcggcgtac
    47401 tcaggaatgc atttaagggt aatgctgagt tcgtgatcag tcggaagtaa tgcaaacatt
    47461 ttcccttgga ctttgaaaac caaagttact tcatcgaacg gcagatgttc ggtaacgccc
    47521 ggtttgttca gacaatattc cctgagttct tcaatattca ttatagcgtt ttatctgatg
    47581 caaaattaat aaatggaatg aaaatgcctt tcagtctgcc aattatataa attaccggaa
    47641 ttgccggaac ttcgttatat ttgcaacgca ttttcaaaag aaatactatg catcggtcgg
    47701 gttttgttaa tattataggt aacccaaatg ttgggaaatc aacgctgatg aattgcatgg
    47761 tgggcgaacg tatttccatt attacctcaa aatcgcaaac taccaggcat cgcattcttg
    47821 gcattgttaa cggcgacgat tttcagattg tatattccga tacacccggc attatcaggc
    47881 ctaattaccg catgcaggaa agaatgatgt catttgtaag tactgcaatt tcggatgcgg
    47941 atattattat ttatgttacc gacattttgg ataaatcaga aaaaaacagc gagtttgttg
    48001 aaagggttaa atccgcacaa attcctgtaa ttgtggttgt caacaaagtt gatctggtgt
    48061 cgccggccga aaccgattca gtaattgctg aatggaaatc ggtttttcct gaacgcgatg
    48121 tagtggcggt gtctgcatta accggttatc aggttgactt gctttttcag aaaatacggg
    48181 atttactacc cgaagggccc aaatggttcc ccgacgatca acttaccgat aaaaccgaac
    48241 ggttcattgt ttcggaaatc atcagagaaa aaattttgtt gaactacaag aaagaaatac
    48301 cctattcagt tgaggttgaa attgaatcgt tcaaagaaac agaaaagatt attaatatat
    48361 cagcggtaat taatgttgcc cgtgaaagcc agaaaggaat tgtcatcgga cataagggag
    48421 aagcacttaa aaaagtaggg acagcagccc gtatcgatac agagactttt ttcgggaaac
    48481 cggtctttct ggaaatgttc gtcagggtga aaaaagactg gcgaaacagc gatagaatgc
    48541 tcgatagttt tggttacaga tagaaaattc ggatattatg gcaggaattg ttgcaatagt
    48601 tggaagacct aatgtcggta aatcgacatt attcaaccgg ttcactgaag cccgcgatgc
    48661 tattgtcgac gacatcagtg gagttacccg tgacaggatt tatggattat gcttatggaa
    48721 tggagtcgac tttacggttg tagataccgg tgggtatgtc gaaagtgcca atgatgtgtt
    48781 tgaattggaa attgccaaac aggtacgcat tgcagtaagc gaagccgatg ttgttttgtt
    48841 catggttgat gtggaaaccg gaatcaccga cctcgatcag aatgttgcag atatgctccg
    48901 gaaaagccgc aaaccggtta ttcccgtagt gaataaagtg gataatcacc tccgtcaaca
    48961 ggatacctat atattctacc agctggggtt gggcgagctg ttttgtattt catcaatcag
    49021 cggacaggga acaggagagc tccttgatgc tgttgtaaaa gcaatccctc cgggtgagaa
    49081 gtcgaatgat aatgatttgc ccagaatatc catcattggt cgtccgaatg tgggtaaatc
    49141 gtcgctcgta aatgcgctta ccggcgaaga acgcaacata gttaccaata ttgccggaac
    49201 cacccgcgat gccatcgaca ccaaattcaa taaattcgga tataatttta ttcttaccga
    49261 tacagctggt ctccgaaaaa aggaaaaagt taccgataac ctcgagtttt actcggttat
    49321 gcgggctgta aaagccatcg acaactccga tgtatgcctg ttaatgattg atgctgtcgc
    49381 cggaatagaa tcgcaggata tcaatatcat gaaccttgtg ctgcgaaagc gtaaaggatt
    49441 ggtattgctt gtaaacaaat gggaccttat gcaaaaggat accaatacgg caaaaagcta
    49501 tcaggaaaat atcttcagga aaacggcacc ttttaccgat ttcccggtgc tttttatttc
    49561 ggcgctgcaa ttacaacgcg tgcataaggt tcttgaaaca gccatgcagg ttttcgaaaa
    49621 ccgtacccgc aaaataacaa cctcaaaact caacgaagtg gtaaccgagg cagtgaatgc
    49681 ttttcatcct cctacttcac ggggcttgca gattaaaata aaatatgctg tgcaattgaa
    49741 taaccccaca ccggcatttg catttttcgc aaatcatccg cagtacataa aagaaccgta
    49801 tcgcaggtat ctcgaaaaca aaatccgcga aaactttatg ttttcaggag ttccaatcca
    49861 gatatatttc agggagaaat aaaatagcag atgttattgg aaaggttgtg gtggaatgag
    49921 cgcatcgttt tacctcccgt cattgcgagg gttgaaaacc cgaagcaatc tgtatttcga
    49981 cgggtgg
//
LOCUS       DAOOQP010000007        47539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_832_length_47539_cov_6.685241, whole genome shotgun sequence.
ACCESSION   DAOOQP010000007 DAOOQP010000000
VERSION     DAOOQP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47539
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_47539_cov_6.685241"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLK75_01450"
     rRNA            <1..301
                     /locus_tag="PLK75_01450"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            371..481
                     /gene="rrf"
                     /locus_tag="PLK75_01455"
     rRNA            371..481
                     /gene="rrf"
                     /locus_tag="PLK75_01455"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(522..1355)
                     /locus_tag="PLK75_01460"
     CDS             complement(522..1355)
                     /locus_tag="PLK75_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37942.1"
                     /translation="MRTSFFILLLLLLWLLAGCNFERNNPPTAEQTVQAMREYVIAAG
                     IFSDSFEESDNAARDADNQLGSLKQFKTGFPVITIEPFDQHTWPKTITVDWGSENYLC
                     QDNRYRRGKIIIMASDYYRNENSVLTINFDNFYQNNHKVEGNETVTNTGRNTDGFIVH
                     TVVVSNGKITTPENKYIYFTQNSERTWIEGESTITEPCDDVYLIAGCQTGISSDSIHY
                     EINVEESLNALVCCKWFRSGLLHVTIEDLPLIEIDYGETDCNNQAVVSFNGVDYPIEM
                     Q"
     gene            complement(1361..1843)
                     /locus_tag="PLK75_01465"
     CDS             complement(1361..1843)
                     /locus_tag="PLK75_01465"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOY37943.1"
                     /translation="MSFSIIVAIGKNFEIGANNDLLWHISGDLKRFKSITTGHTIIMG
                     KKTYESLPVRPLPNRRSIVLSDIENDSIPGVVVASSIEEVKKLCNASDENFIIGGGMV
                     YRQFLPLAEKLYLTLVHRSYTADVYFPEINYNDWNCISAEEHPEHDPPFSYKIFTRKK
                     "
     gene            complement(1862..2656)
                     /locus_tag="PLK75_01470"
     CDS             complement(1862..2656)
                     /locus_tag="PLK75_01470"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOY37944.1"
                     /translation="MKAYLDLLSHVLQHGVTKNDRTGTGTISVFGYQMRFDLSEGFPL
                     LTTKKLHLKSIIYELLWFLQGDTNIKYLKDHGVSIWDEWADENGNLGHIYGYQWRSWP
                     SAEGKVIDQISELINSLKNNPDSRRHIVSAWNVGDLENMALPPCHAMFQFYVSGDKLS
                     CQLYQRSADIFIGVPFNISSYALLTLMVAQVCGYRPGEFIHTLGDAHIYLNHINQVKL
                     QLEREPRTLPTMEILNNRTSIFDFQFEDFLLSNYNPHPHIKGQIAV"
     gene            complement(2929..5370)
                     /locus_tag="PLK75_01475"
     CDS             complement(2929..5370)
                     /locus_tag="PLK75_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY37945.1"
                     /translation="MKLYFLITVALLHLFFSTVSNAQEGPRGNAPADGIIFGTIIDNK
                     SNEPIEYANVSIYSLRDSTLVSGGISNKKGVFQITSLRYGKYYVDIDFIGYKKIRVND
                     VFINPENNNVSLGKIKLSPAVEMLNEVEIEAEGPSVEYKIDRKVITVDKNLSAAGGTA
                     VDVLENTPSVQTDIDGNVSIRGNSNFLVLIDGRPSPVQGSDALQQIPAGSIQSIEIIT
                     NPSAKYDPDGVGGIINVILKKDKRTGINGLFSLSYGSFNTLGGDFLVNMRTEKFNFFV
                     GGNYNNRLNKSSGNSEQRTYASDTLFVYKESQNNRGREDGKLRGGFDFFITPNDVITL
                     SGNIGKDGMHSGADEYFTSYWLRDNMVSDSISYNNVNSGQHNGLYYSADLNFQHKFLK
                     KGHELTAYTYFDYDDNDENDQYTQYFSATDTNSYRTREFSISRNLRGQIDYTLPLFEK
                     GKLEAGYQVKQKWSSNNYDYQSLIVDDWVDSLTNNYSFDQNIQSGYLTFSHEVGKFGY
                     MLGLRSEYTNRTIHQAAVIVPFDSVYKAFNVFPTLHLSYQLPWEMQVMTSYSRRINRP
                     WDFFLDPYPEVTDAYNIRQGNPMLAPESTNSAELNLQKRFGNNFVSIEGFYRYTTNKF
                     ERIKKVSPDNSEIMISTWENIGKDMSLGGELMGNVNLTKWWNLNLSGEAYYYEIVADA
                     SGNVNSNNTFTWGARMNNTFKIKKTGTTFQLSGFLRGNSITSQGTTKGSGMVNIALRQ
                     DFLDRSLSVTFNLRDIFNTMNHEFISETESFYSYTYMNRKSPTFNITITYKLNDFTKR
                     KDKTSEYGQDEGDEM"
     gene            complement(5451..5687)
                     /locus_tag="PLK75_01480"
     CDS             complement(5451..5687)
                     /locus_tag="PLK75_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37946.1"
                     /translation="MKVTLLTPEKTLFEGQATFVEVPGAKGRFQMLDHHAPIVSILSA
                     GKVRIVLPDNSEKLFDIQGGVIELKNDIITILAE"
     gene            complement(5690..7207)
                     /gene="atpD"
                     /locus_tag="PLK75_01485"
     CDS             complement(5690..7207)
                     /gene="atpD"
                     /locus_tag="PLK75_01485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOY37947.1"
                     /translation="MEQIIGEIVQVIGPVVDISFDRSGSDLPNIFTALEIQREDGNVL
                     VVECQQHIGEHTIRAIAMDSTDGLSRGMKAIATGTTIKMPVGDQVKGRLMNVVGKEID
                     GMQALDNRQGYPIHRDPPKFEQLTTSSEVLYTGIKVIDLIEPYAKGGKIGLFGGAGVG
                     KTVLIQELINNIAVGYNGLSVFAGVGERTREGNDLLREMIGANIVKYGSDFIKSMEDG
                     SWDLSKVDSEAIKDSKLSMVFGQMNEPPGARARVALSGLTIAESFRDGDEKSGGRDIL
                     FFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGEMQERITSTKHGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKIAGLGIYPAVDPLDSSSRILTPEIVGQEHY
                     DTAQRVIQILQRYTELQDIIAILGMDELSEDDKIIVHRARRIQRFLSQPFHVAEAFTN
                     IKGAFVKIEDTIRGFKMILDGEVDQYPENAFLLVGKIEEVIEKGEKMLAAAKELN"
     gene            complement(7306..8277)
                     /locus_tag="PLK75_01490"
     CDS             complement(7306..8277)
                     /locus_tag="PLK75_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOY37948.1"
                     /translation="MHSFEELHKIINSYFQQYEWAKAPENLYKPIEYSLSLGGKRLRP
                     ILTLMSCELFSDNYNKAIDAAAAIEIFHNFTLLHDDIMDKASLRRGIPTVHARWNANI
                     ALLSGDAMSIIAYRHISRISYKMNECLDIFNDTALKICEGQQIDMDFEERADVTVDEY
                     LHMITYKTAVLIACSLKIGSLVGGATSDEAEKMYDLGLKLGMAFQLQDDLLDVYGDEK
                     IFGKNIGGDIVANKKTYLLISALNADSKSTRKQLTTAMNEIFLANDDKISKVTDIYNQ
                     LGIKELTLKTITAYFSEAIGILNSIPIKENKKIVLRDLILSLKSRSF"
     gene            complement(8277..9251)
                     /locus_tag="PLK75_01495"
     CDS             complement(8277..9251)
                     /locus_tag="PLK75_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY37949.1"
                     /translation="MYTTASRHSFKRKILITGGAGFIGSALAEKLASDNENLVVVVDD
                     LSTGRIEKIPTGYDNLRFIKCDVNEYRDIVEVMLSYQFHYVFHYAAVVGVHRTQEHPV
                     NVLKDIRGLENILRISKNTGVKRVFYASSSEVYGEPVELPQNAHTTPLNSRLPYAIVK
                     NLGEAFLRSYQREYGLNFTIFRFFNTYGPKQSKDFVISKFIYNALHNHDITIYGDGSQ
                     TRTFCYIDDNIESTVLIASKDMYINDVINIGTDTETPILELADTVIKLTGSSSKIVFL
                     PPLAEGDMTRRCPDNKGMREVLNRDLISLEEGMKRILEQGLFEFKLQH"
     gene            complement(9274..11670)
                     /locus_tag="PLK75_01500"
     CDS             complement(9274..11670)
                     /locus_tag="PLK75_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOY37950.1"
                     /translation="MFWTKIAGFILRNRLPLLIAITAITGVMIWQTTRVRMDYHYANL
                     LSEKDPIYLDNKEFKATFGEEANAIFIGIKDEKFFEAGHFNNFTRLCNDLKKIPNVKS
                     VLSIPQAVNIRQITVETGDSKKRQFEIYPLFSDSTYTQQQLDSLKTVFMNLPFYNGLL
                     YNHEKHVFLLSVSLKNEILDSPLRIPAVKQIEKTVEEYSTANNIPVFISGHPFIRTKL
                     MTMIRSEIKIFIVLAALISAIILFLFFRNIKIILISIVVVGTAVVWATGLMGLFHYKI
                     TVLTSMIPPLLIVIGIPNIIYLLNKYHTEVRANKNKILALQRAIRRKGTAVFLSNLTT
                     AVGFATFIITNSRILWEFGLIASLGVLFSFVLSLIVIPVVSSYLPVPSEKYTRHLDNK
                     ITTWVIRKAELITNSHRKPLYIIIASVMLLSVLGIINIRQTGYLMDDVPHKKKMYVDL
                     KYLESCFHGVSPLEIMIKTQDTLSGNQFITQIQKIDSLQISLLKHKELSRSVSVADAL
                     KFLNQAYSQGKSVAYRLPDNPKTYETIFKRLPQKFDFNNQFIASFIDSSKTITRVSLN
                     VEDVGTKKMKVLIPFIKKEVDQIFPPEKYHTVITGSTILYFTGTTYLTKNLFSSLSLA
                     FIVIAVLLYWMFRSVRMTLLGLIPNMIPMILTAAIMGAFNIPLKPSTIMVFGIAFGIS
                     VDGTIHFVNKFRYEYIALNKKIGVAVNEAIQEVGISLVYNATILILGFSIFIVSGFGG
                     TKALGILVSIALFASMICNLLLLPSLLLSLEKFYDRKTIGSVGIDAYDEETLSETNIN
                     "
     gene            complement(11822..14044)
                     /locus_tag="PLK75_01505"
     CDS             complement(11822..14044)
                     /locus_tag="PLK75_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY37951.1"
                     /translation="MLVFQVILVVLCILAITDLTVGVANDAVNFLNPAIGSKASKIKI
                     LFVLVSIGVLIGATFSSGMMEVARKGIFNPNNFYFNEVMFIFLAVMLTDVLLLDAYNT
                     LGLPTSTTVSLVFALLGASVSMALIKMNIQPDALSISEYINTSKALAIITGILISIVI
                     AFTVGAFIQWLARLIFSFNYEAKIKYFGAIYGGIAITVIVYFMLIKGLHGASFMNEEI
                     NTFISTHTLEILLYSFVGFTILFEILYLLFNINILKIIVLTGTITLAMAFAGNDLVNF
                     IGVPMAGWSSYQFFTESGVANPESFTMGALSGPVRVNTFILIAAGVIMVLAIWLSKKS
                     RSVIKTSVDLSRQSEGHERFGSSLIAREIVRMGVTSAQTFGKIVPSGLKKRIAKQFEP
                     RVYSSAEIEQRPAFDMLRASVSLIVASILIALGTSLKLPLSTTYVTFMVAMATSFADK
                     SWGRESAVYRITGVISVIGGWFITALLAFLLSSIILLIVYFGGLIAVIALVAVAVFFF
                     IRSNVIHKKREETESEKSQTISNSFIEETKLKLQEITGDISNCYTDAIDAVCKFKHKK
                     LKKRFRLSSEIEEKVQQYKNDIYPTLSCSNINFENSGIFYVQMIDYLNESANCLKRII
                     DPAFRHVDNNHKELTPAQIEGLTQISNPISFYFTEIINLIQKNSFSESEFESISVTYG
                     NINSVIENVRSEQIKRIRSKEDPTRSSMLVIAMLLETQNLITDIQKMLKAYIDFRKSF
                     "
     gene            complement(14156..16372)
                     /locus_tag="PLK75_01510"
     CDS             complement(14156..16372)
                     /locus_tag="PLK75_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY37952.1"
                     /translation="MVLLYVILVVLILLAVASLVVGVSNDAVNFLNPAVGAMTASTKL
                     LFLLVSVGVVLGATFSNGMMEVARKGIFNPDNFYLTEVMFIFLSVMLTNVILLDAFNT
                     LGLPTSTTVSLVFALLGGSIAMALIKMNTQPDALNISEYINTSKAMAIIFGILFSIVV
                     AFTIGAIAQWIARVLFSFNYEPRMKYLGAIFGGISITIILYFMIIKGLGGSSFMTPEL
                     NEWLNKNTWLFLLYFFVASSVVFEILYLLFKINILKVVVLAGTLSLAMAFAGNDLVNF
                     IGVPLAGLDSLSTFRESGCNDPSSFTMTGLSEPMKANVLYLIAAGLIMVLALWFSKKA
                     RNVIKTTVNLSRQTEGHERFGSSLLARQIVRMAVSTGQAVNKVMPAILVRKIEKQFEP
                     KVYSAADSKERPAFDMLRASVSLVIASILIALGTSMKLPLSTTYVTFMVSMATSFSDK
                     AWGRESAVYRITGVLSVIGGWFITALISFVLAGILLVAVYFGGLVAIIILILLTAFFF
                     IKSNLVHRKNEEKELEYQHTVSNSFTEETKLRVCELSELITGCLIDSINGVCDFKLKK
                     LKKRNKLSKEIDQMTKAYKNDIYATLSKAGISFENSGVYYVQMIDFLRESSGVLVKIT
                     EPAFEHVSNNHKELTEKQIEGLKRILSPMEFYFVEYNNMLQNNTLENFAELSATSANI
                     ENIIEKVRSEQIKRIRSKEDPTRSSILVINILQQCQNLLGDMQKMLKAYINFRKSF"
     gene            complement(16484..17209)
                     /gene="lptB"
                     /locus_tag="PLK75_01515"
     CDS             complement(16484..17209)
                     /gene="lptB"
                     /locus_tag="PLK75_01515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOY37953.1"
                     /translation="MKLYTENLYKRYGKRMVVKDVSVEVNQGEIVGLLGPNGAGKTTT
                     FYMIVGLIRPYEGKIFVDDKEITGDPVYKRARLGLGYLAQEASVFRKMSVEDNILSIL
                     EMSKLSKREQKEKTVRLLNEFGLTKIRKSKGYQLSGGERRRTEIARALAIDPKFILLD
                     EPFAGVDPIAVEDIQQIVMHLKNRNIGVLITDHNVQETLSITDRAYLLFEGSILKSGT
                     AEELSEDEQVRKVYLGQNFELRR"
     gene            17415..18839
                     /locus_tag="PLK75_01520"
     CDS             17415..18839
                     /locus_tag="PLK75_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY37954.1"
                     /translation="MLRLIFAFLFLSVAVPIVAQKQWTLKDCIDYALENNLEISQQSL
                     YIDMYRNNLLSSKLSVLPSLNAGVNQNFSFGRSVDSYTNEFSEENISSSNASISSSVT
                     LFNGLQQYNTIRKSKADLEAGLADMETARNNLSLSVTSAYLQILYSVEMLETATLQME
                     VTRSQLERTIKLVNAGSLPVQSQLEVEAQLASEEMSMVTAQNQLDMALLNMVQLLELN
                     DVADFSIVKPDFSGVIGHPALMTVDEIFNEAVMNMPEIKSADMKLKSSEYQLAIARGS
                     RSPQLSVSASYGTGYSNARKMIDDITPASPVLTGYAVDAYGNYSDVYQYSFDYSYITR
                     PFSDQIRDNASASVSFGLSIPIFNGWYTNTRISNAKIDVLQAQMQFSIAERQLYKVIQ
                     QAHSDVRAALKKYDAAVKAYHASETSFQYTQQKFDLGLVTSLDFNTAKTNLNKLKSEM
                     INAKYDMIFKMKILDFYRGVEIKL"
     gene            18887..20236
                     /locus_tag="PLK75_01525"
     CDS             18887..20236
                     /locus_tag="PLK75_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY37955.1"
                     /translation="MKTKRKKLYRWLILGLIVLIVLLIVARKAGWMGGEEINKVSVDK
                     VAKRTIVETVTANGKIQPEMEVKISPDVSGEIVELDVKEGQQVKKGQFLLKIKPDLYE
                     SAVDRSIAALNGAKANLSNSRARLEQTQAQFNQTELAFNRNQKLFDQGAISQAEYENS
                     LASYQMGMADLNAARENVKAAEFTVMSAEATVKEANENLYKTSIYAPVDGTISLLNVE
                     LGERVVGTEMMAGTELLRIANLDVMEVTVDVNENDIVKISINDTADIEVDAYLNKKFL
                     GVVTEIANSATSTGLSADQVTNFKVKIRILQTSYADLINANNKFPFRPGMSATVDIRT
                     NAAINVICVPLMAVTTRPDSASALQVKPGAKDDDDRSTSDITLKEVVFVYKDGKAVMK
                     NVVTGLQDSFFIEIKDGLTENEEVISAPNSLISKMLKDGMTVTKVDKNALFSETKPK"
     gene            20237..20950
                     /gene="tsaB"
                     /locus_tag="PLK75_01530"
     CDS             20237..20950
                     /gene="tsaB"
                     /locus_tag="PLK75_01530"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOY37956.1"
                     /translation="MPVILSIETSASTCSVAIANDGRNISVKENHVDKSHASILNVLI
                     REAVAESGIPMGKIDAVAVSKGPGSYTGLRIGVSSAKGICYALNKPLIAVDTLKIMAI
                     MASQVFLQKLDEELLICPMIDARRDEVYTALFNSRTEKLNETTAVIISPDFFGDIDPE
                     IAILCCGSGAPKCNEIVKRKNVVFSDSIFPSAEFMCQPALDLYSKACFEDVAYFEPYY
                     LKDFIATVPKNKIPGIIAR"
     gene            21042..21311
                     /locus_tag="PLK75_01535"
     CDS             21042..21311
                     /locus_tag="PLK75_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOY37957.1"
                     /translation="MKENVHPKNYRLVAFKDMSNEQVFITRSAAQTKETIDIDGVQYP
                     LFKLEISNRSHPFYTGKMKLVDTAGRVDKYRSRYQKHIDKNNQNS"
     gene            21402..22574
                     /locus_tag="PLK75_01540"
     CDS             21402..22574
                     /locus_tag="PLK75_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOY37958.1"
                     /translation="MKNYILSDAGLHKNLLPITFTRPVSEIRSGILTNTQRWQHFLGE
                     KCSFLTEIYLQKKFPASTGQANIWINGGVVADKHIAERVLNLQQGSLMCNGVLIAAVC
                     VGTPPVIFDNYPEPVQVYDGNVIVIQQVYDIFGLAAKCIDIDFELLAGGRVSNKLSKT
                     NTVIGDNPVFLEEGAIAEASVFNTTNGPIYLGKHCEVMEGSMIRGPFCLLDHSVLKMG
                     AKIYGPTIIGPHSKIGGELNNVVILGLSNKAHDGFLGNSVIGEWCNLGADTNNSNLKN
                     NYAMVKLWNYEKKSFLNTGLQFCGLIMGDHSKCGINTMFNTGTVVGVSANIFGSGFPR
                     NYIPSFSWGGASGFVQFGYEKAIEVAGLVMKRRNMELDNTEKEIFKYLFDNNDHKF"
     gene            22657..25116
                     /gene="lon"
                     /locus_tag="PLK75_01545"
     CDS             22657..25116
                     /gene="lon"
                     /locus_tag="PLK75_01545"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOY37959.1"
                     /translation="MNELNDFLINALSLSGIENDGEVIPILSIDDDKSEENDSDSYPE
                     KLPILPLRNTVLFPGVVMPLTVGRSKSFQLVKEVYASNKMLGTVSQLEASIEDPEIDD
                     LHSTGTLAKILKIIEMPDETISVIIQGKKRIEINEIVEKEPYFMGYYKILPDLKPAGS
                     DKEFSAIVSTLKDLSLKIIKLSNSIPKEVAFAIKNIDNSSFLINFICSNSDIQPIDKQ
                     HLLETDDLKERAIHLMELLTRQIQMLELKNDIQAKVKKDLDQQQKEYLLHQQMKTIQE
                     ELGGNPIDQEIKEMTEQAKKQKWNDEVKTHFEKELSKLQNLNPAAAEFSVQINYLHTL
                     LDLPWGEYTADNFDLKNAATVLDQDHYGLEEVKKRILEHLAVLKLKGDLKSPILCLVG
                     PPGVGKTSLGKSIARALGRKYARMSLGGLHDESEIRGHRKTYIGAMPGRIIYNLKKVK
                     SSNPVFVLDEIDKVGKDFRGDPASALLEVLDPEQNSEFHDNFIEIEYDLSKVMFIATA
                     NTTNTISPALLDRMELIDVNGYIVNEKIEIAQKHLIPKQLEAHGLTKDHVCFDNKTLE
                     YIIENYTRESGVRTLDKTLAKLIRQIALKVAVEETYNPKMDAQAVENLLGSKKYFKEK
                     YQDNETAGVVTGLAWTATGGEILFIESSLSVGKGTVTLTGNLGEVMKESAIIAHEYVK
                     AHSELLDIPSGVFEKWNIHVHVPEGAIPKDGPSAGVAMAVSIASAYTQRKVKKSIAMT
                     GEITLRGKVLPVGGVKEKILAAKRAGISAILLSNENKKDVEEIKPMYIEGLRFIYVDT
                     ILDVFKAALTLQKVKNPKNIV"
     gene            complement(25122..26453)
                     /locus_tag="PLK75_01550"
     CDS             complement(25122..26453)
                     /locus_tag="PLK75_01550"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOY37960.1"
                     /translation="MKTLIKNASLVNEGEIYVANVIIQDDVILKISRKEESGHFDEVI
                     DATGLLLFPGVIDDQVHFRDPGLTTKGDIFSESRAAAAGGITSFMDMPNTIPQTTTME
                     ALHNKHIIAAHSSLINYSFYLGATNDNTAEIEKTDPLIVCGIKMFMGSSTGNMLVHSN
                     EAVSAVFAHAPVLVAVHCEEDSIISRNLAKYKALYGDNIPMHHHADIRSEEACYASSA
                     RAVELATKHNARLHILHLSTEKEISLMQQIPLNKKTITGEVCVHHLWFSKNDYNRLGS
                     RIKWNPSIKTENDRLSLIQGLNSGYIDIVATDHAPHTIAEKLSHYSQCPSGAPMVQHS
                     LVVMLEMAAKHNFSYQQVADYMCHKPAALFQIQKRGYIRESYYADLVLVNPNAPTTVS
                     PGNILYKCGWSPLENQTFSHGIVSTWVNGKKVFDKGKIIEVQAAMPLRFER"
     gene            complement(26468..28903)
                     /gene="priA"
                     /locus_tag="PLK75_01555"
     CDS             complement(26468..28903)
                     /gene="priA"
                     /locus_tag="PLK75_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOY37961.1"
                     /translation="MKYAQIIIPVALNGSFTYIVPDSLCSIAEQGSSVVVNFGKSKLY
                     TGIISGFTEHPDSQLKLKEILSCSSKPVTNMAQMELWNWVAQYYCCSIGEVMKAALPA
                     GIRPDSESRYIKNDTFSDYHSLPEKQRIVLNYLSPEKPKGIQEIIRNTGIRNPIPDLN
                     KLAERGAVQCVQFLSGVYKPLYRKFISLNFISAEQAHSVLDSLKRAPKQAQMLAFLIE
                     QAGEEYGSFQAELSEVLTSTGLDAQTVIALSKKNIISFVLKETSRFQQTENTSLLKEL
                     SAVQLNCLKEIKAVFNENKVCLLHGVNSSGKTEIYLHLIDEAVRNNHTALYLLPEIAI
                     TTQIVHRIKSVFGNIVGVYHSKYSDSERVEVWKKIQDGQLKVIIGVRSSVFLPLLNPG
                     IVIVDEEHENTYKQSDNSPRYNARDIAVVMAEKCGAKVLLASATPSVESYFNAISGKY
                     GLALLSTRYGNVEMPRIVIVDTLQARKRKSMQLHFHPLLIEEVKGALHKSEQVILFQN
                     RRGFSPYIECNECGWVATCKNCNVSLTFHKRRQQMLCHYCGYSTGLVRNCPDCQSTSI
                     KTMGFGTEMVESEARIFFPEARIERLDYDSVSTRAKYEKLIADFENRDIDILIGTQMI
                     SKGLDFDNVSIVGILNADNMLNFPDFRAFERSYQLMSQVSGRAGRREKRGKVIIQTSM
                     PEHDVIKYVVEGNYDGFARHELTERNTYRYPPFYRFINLKIRHRNYSTLNSAAISLKN
                     TLEQLLPNRVQGPHEPIVNKVQKWFIMNVIIRFEAGISVRKTKDFILSSIEAIKKHPE
                     YKGVDISIDVDPL"
     gene            complement(28934..29914)
                     /gene="pfkA"
                     /locus_tag="PLK75_01560"
     CDS             complement(28934..29914)
                     /gene="pfkA"
                     /locus_tag="PLK75_01560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOY37962.1"
                     /translation="MTKFKRIGVLTSGGDAPGMNAAIRAVVRSAIYYGMSVTGIRRGY
                     AGMILGDFMEMTSRDVGNIIQRGGTILRTARSDEFRTKEGRYIAYRNLVEAGIEALIA
                     IGGDGTFKGAKLFTEEYNYPIIGLPGTIDNDLYGTDMTIGYDTAINTAIQAIDKIRDT
                     ASSHDRLFFVEVMGRDAGFIALRSAIGCGAEDVLVPEVETNMDEVCANLANSVQKTKN
                     SGVVIVSEGDDAGGAMKIAEIVKQRLPDMDIRVSILGHLQRGGAPSAFDRYLASKLGL
                     AAVEALLDDQRSVMVGLIKNETALIPFNQAIKHHKDINRKMLEMLYILNT"
     gene            complement(30065..30484)
                     /locus_tag="PLK75_01565"
     CDS             complement(30065..30484)
                     /locus_tag="PLK75_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HOY37963.1"
                     /translation="MDCSVFTDKIIIPSDTMLRNALKATYPLWRNIEDYIMTKQTGLQ
                     TEWAFPGAKYGWSYRILDKKRVIVYLLPRDSFFMAAFNIGAKAFEAIMGTDVSATIKT
                     ELSEAKVYAEGRGIRIPVHDSAIINDILTLVEIKLMF"
     gene            30606..32141
                     /locus_tag="PLK75_01570"
     CDS             30606..32141
                     /locus_tag="PLK75_01570"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOY37964.1"
                     /translation="MNGEEFFKTLISHCKEYGFIFQSSEIYDGLSAVYDYGQNGAQLK
                     NNIRDYWWRAMTQMNEEVVGIDAAIFMHPTVWKASGHVDAFNDPLIDNKDSKKRYRAD
                     VLIEDYLAKLEDKVNKEVEKALKRFGESFNEKLFRETNPRILEYQQKSDEVRAAMTTC
                     LEENDLAGIKRLIEELGIVCPISGSRNWTDVRQFNLMFDTKMGSVAEGADTIYLRPET
                     AQGIFVNFLNVMKTGRMKLPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQYFVKPG
                     SELEWFEKWREKRMKWHHSFDFDTAKYRWHKHDKLAHYANAAYDIEYAFPFGFKEIEG
                     IHSRTDFDLMRHQEFSGKKMNYFDSETGESFVPYVVETSIGLDRTFLMVLSAAYTEEQ
                     VGNEMRIVLKIPAPLAPVKAAVMPLVKKDGLPEIARGIIDGLKFDVVCQYDEKDSIGR
                     RYRRQDAVGTPFCITVDHQTTTDNTVTIRYRDSMEQERIPIAKIGDIVREKADIRNLL
                     KQL"
     gene            complement(32254..32556)
                     /locus_tag="PLK75_01575"
     CDS             complement(32254..32556)
                     /locus_tag="PLK75_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37965.1"
                     /translation="MKMIYISCNVSTTHIITELLDAAGIDEYQISDDVAARVFTGKHR
                     MNTSVWPGYNNIIFVPLCDDKADEITNKLRQYNASVQNTDELVSVGSWSLDDYFND"
     gene            complement(32558..35656)
                     /locus_tag="PLK75_01580"
     CDS             complement(32558..35656)
                     /locus_tag="PLK75_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY37966.1"
                     /translation="MGLPKLTVQNPVTTAMVFIALVIFGVISWISLPRDLMPDIELPA
                     ITVITIYPGASAEDVESQVTNPLEVVLSGTENLKKLSSSSRENVSFISMQFDWGTDVS
                     DAVNSARDLMELAKSKLPVDAMSPYILKINSSMIPVVVYTIQADISYNGLEKIAYEQI
                     IDPLKKVPGVGSAFIIGQPSREISINVDPVKLKAYNLNITQIATILQAENITIPGGNV
                     RFDKYDFAVRIPGEVKNTQDIGEIAITSFNNRIIRMKDIAEITDGYREKDEKAYSDGK
                     TAVGLFIQKQTDANTYEVYTAIVEEMAGIQKNLPADVKTNIVFNTAEIIKEVTSNLSS
                     TIWYAGLFVLIVVLIFLREWRNSLIIILSIPVSMVFAFITMKLLGYTMNIFSLISLIV
                     AIGMVVDNSIVVLENINKHIENGARPKQAAIFATGEMGNAITASTLTTICVFIPLMFT
                     GGLVGVLFKQLAILVSVTMAASLLTALTLTPMLSSLLLKNQAQHRQKRSRLFRSSENI
                     FNSIENGYRSSLKYVVRFKYLFISFALLLFLLTIFIFTRLGNNYIPDLDAGDIIATIE
                     LEVGTNVDETERVAKKVEKIFNDEIPEMVSQYTIVGQSESNLLTGIGFKEGKNRATIS
                     AHLCLPQYRQRTAIEIAEVIRLRLAEIPEIENYQVIGGSMLQNMLLGNSKPVEIKISG
                     KSFEKINETALYFVELLKSDQRFSNVESSIDLGKLEYVVDIDKDKASLMGLNTAMIAL
                     QIRNGIYGAEAGTYKEEGDDYKIVVRFGKDYRNSIEDLKKINLSTLMGSQVALSNVAE
                     IKESYGPLEIKHETQSRFVTVGAELNGITLSEAAQIVREMISKTDADHAVNIAISGEV
                     LDKEESYSNLQWIFIIGLVLVFMVMASQFESLKDPFIILFAIPFAIIGVVLAFVITGI
                     QLNIVSFVGVIMLLGIVVNNGIVLVDYTNLLRARGWSITTAALDAGKSRLRPVLMTSL
                     TTILAMIPMLFSKSMGSELWIPLAITMIGGLTVSLLITLYLIPCIYLSLNQKALKNEK
                     NR"
     gene            complement(35659..36696)
                     /locus_tag="PLK75_01585"
     CDS             complement(35659..36696)
                     /locus_tag="PLK75_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY37967.1"
                     /translation="MKKFAIYLFALILLIISCKRDDKQPQKVENNPVVKTTIAVEKQF
                     SPELEYSGTFFPNREANLGASMPGKIEKFFYPEGTFVTSGTLLVEMSAELLTQALIEY
                     DAIKKDFERVERLRAKGSISEIEYDHLKAKLEASEVKTEMLHKNTSVVAPFDGIIVEY
                     LVEEGENYFFNLNFDPGYSNTSGILRLMQINPLVVKIQVNEKELQQLKSGQKVSIMCD
                     AVSGNGLTGFVKYIKPMLSTVTRTAEVEIEVPNPGNRLKPGMFARVLLHTEGSSAVFV
                     PVDAIYRQPGTPDDFIFVVHSGVAHQMAVKRIAIIDQWVAVSGISKGDTIVTQGKNKL
                     KDNQQVIIKNN"
     gene            complement(36709..38190)
                     /locus_tag="PLK75_01590"
     CDS             complement(36709..38190)
                     /locus_tag="PLK75_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY37968.1"
                     /translation="MTYFKRIALVQLLIWFIANPLHSQTDTLYLNLGQCRDMAFKHNS
                     KIKIAECRTQAATEMRKASFTKYFGEFGFMGQYQYSNRQISLFNQDLFLPVVPFWAID
                     ENTGTLREDILENPLLNGIVSNPLTGEIFYDSQGNPAFWLYGYLPSDQLKFGTHQNFM
                     FGPSLLQPIYLGGKIRNMVKVAEAGEEIMKSKQLLDEDDVIVLTEEAYWRCVSVQEQK
                     ALAYKYRELLNHLVGDLENLYAEGIITYNDVLKAKLKLNEAELSCLKADNGLALSQMA
                     LLQIIGLPLNTQISLDGNLDKLPVLPNPSGSYETALQNREELHILDNTMNMAIAQKNI
                     ALSRFLPNIMASANYLFVNPNPYNAFEKEFGSDWSLGVTVQIPLFHWGDRLHTMRASK
                     KIIEISQLMKQDAEQMIQLELEQAYFVYTETMKKYDLCEKALIQSKENLRMANDAYQE
                     GMLTLADLLEAQVLWQQAYSELIQAKTDLKLQEINYRKKTGQL"
     gene            complement(38195..38776)
                     /locus_tag="PLK75_01595"
     CDS             complement(38195..38776)
                     /locus_tag="PLK75_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY37969.1"
                     /translation="MNRSDKVRENLIKSARQMFARFGFEKTTMNEIAIEARKGKSSLY
                     YYFTSKEEVFQAVVEYEADMLREKLAAAFREPENLLDKMRNYIVVRFNEIIKLGNLYQ
                     ALRNDFLNHLEFVQKARLKYDKLELEFFTLVLKQGCENGVFTIENPEHVAKTFHLTIK
                     AVELPLLISYEQEVFEKQLNTIIDIVFFGLVKR"
     gene            complement(38970..40841)
                     /locus_tag="PLK75_01600"
     CDS             complement(38970..40841)
                     /locus_tag="PLK75_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOY37970.1"
                     /translation="MNINFLRSITPRWVIFLIDLMICLFSIVFAYYLRFNFKVPAYEI
                     ARFVYVLPMVLSIRAISFIVSRSYAGIVRHTSTRDIQRIILVSAISSAILVILNVITY
                     YLQKRFLIPMSVIIIDFITTAFILSFSRLLVKAVFLEFNNPRRKAFSVAVFGTEELAL
                     ITQRTIDRDTESRDKVVAFFDFGSKMTGNKIEGIRVFDISDLETQIKKQFISTLIIAK
                     KQIPVEIKQKITDICLDNDVEIKVIPDVSKWINGELSLHQIQNIKIEDLLQRPEIKMD
                     KKHIRKQLLDKTILITGAAGSIGSEIVRQLTNYNPKHLVLFDQAESQLYDIELELEEK
                     LGFHNFTIVIGDITNAELVERVFTSFNPEFVYHAAAYKHVPMMEKNPGAAILNNVTGT
                     RLLADASLKFKVLKFVMVSTDKAVNPTNVMGASKRIAEIYIQSLNQFKKCKFITTRFG
                     NVLGSNGSVIPRFKRQIESGGPITVTHPEITRFFMTIPEACQLVLEAGSMGEGGEIFI
                     FDMGESIKIVNLAKRMIQLSGLKLGEDIQIRFTGLRPGEKLYEELLATKENTVPTYHP
                     RIMIAKVREYDFNTIQDHIETLVQMIDNSENMALVKKMKEIVPEFISQNSPYEVLDH"
     gene            41008..42066
                     /locus_tag="PLK75_01605"
     CDS             41008..42066
                     /locus_tag="PLK75_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37971.1"
                     /translation="MIRLKIIGLLLLFGLLSSCGIRVKYDEADKKCRNAPDVVLKKFD
                     GGVFSIEIPESWKVITAGNCETFSFCAYDSIKPANRVFLLGEIGPVYQHIEKKREDFQ
                     SIQDMGLDIGYFDMPVVLPFTPECYFENLYLMFETEWAKAMKPICPDFHNTIIINSET
                     ITPVMSDGISKVLRAIHSSNDTIYQTLILLNLAPFYEEMGKTKKGLGKAYIVLGVNAE
                     FNDFEALLPLMLQIAASFHMNENYIQNCSVGASAFAMEQMKLSKSLTPYVTIMRDDWN
                     ARDKSMKYIILKSCDSLLNKIRIFDPNTGTPYLVSASFFDKYINSKEEFEMNKLKILS
                     VSDTALWLKTPLDSSYIR"
     gene            complement(42077..42802)
                     /locus_tag="PLK75_01610"
     CDS             complement(42077..42802)
                     /locus_tag="PLK75_01610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY37972.1"
                     /translation="MIIDYNKIALNQVLFIDIETVPAVPVYEELSESFRKHWERKSSF
                     FRNADESASDVYQRAGIYAEFGKIICISAGFITERNGDKHFRVKSFSGDTEKELLTEF
                     SAMLDSFAPKGDKYFCGHNIREFDIPYISRRMLVNRIPLPEMLNNAGKKPWDMKLLDT
                     MELWKFGDFKNYTSLELLANLFGIPTPKDDIDGSMVADVYYNEKNLNRIVTYCEKDVL
                     TVAQLLLSYRLMPLIEEANIIKA"
     gene            complement(42806..43417)
                     /gene="udk"
                     /locus_tag="PLK75_01615"
     CDS             complement(42806..43417)
                     /gene="udk"
                     /locus_tag="PLK75_01615"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOY37973.1"
                     /translation="MLIIGIAGGTGSGKTTVVKKIMERFSEDEVSVIPQDSYYRDNSD
                     LPLEKRQEMNFDHPDAIEWSLLIDHIKRLKRGETVMQPMYSYLTCTRLEDVIPLSPHK
                     VVIIEGILILGNKELRDLMNLRVFVDADADDRLSRVIQRDIIERGRSVNKVLERYEKT
                     VKPSHLQFIEPTKRYADLIVPQGGNNSVAIELLTHFIEIKLKN"
     gene            43477..44454
                     /locus_tag="PLK75_01620"
     CDS             43477..44454
                     /locus_tag="PLK75_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOY37974.1"
                     /translation="MLYPLKFRPIYKQRIWGGNRLASLFNKKSAPANCGESWEISGLP
                     ENISVVTNGYLKGNNLQELIEVYMGDLVGEKVFDTFGLDFPLLIKLIDATEDLSFQVH
                     PGDDYAAENLESYGKSELWYVLHAAEGAKLVSGFNRTITRETLLEKIGNHSLPEVACY
                     ENAQYGDVFYIPAGRMHSLGKNIVLAEIQQASDITYRIWDFDRFDANGKKRELHIEQA
                     LDVLDFSKTVDPRTLYSKLPDKGSSIITTPHFSCNIVCLFNNLERSYADKDSFVVIIC
                     VLGEVNLHYNNEKYNLKKGETILIPAEISDITYEPDGYTEFLEIFVLME"
     gene            44554..45783
                     /locus_tag="PLK75_01625"
     CDS             44554..45783
                     /locus_tag="PLK75_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOY37975.1"
                     /translation="MRTLVLLIVFFSVAIVFSACKKQNNEIVDLKLTTQQKNMVVSGN
                     HFAFNMLREVSESETAPKNIMISPLSINFALAMTANGAKNNTLSQMLTTMDFEGYEME
                     DFNEYFRYLKSELVALDPRVELSIANSIWYRNNFTVLQSFLDVNQNYYDAEVASLDFS
                     SGDALNTINDWVNDETHGKIERILEEIGPDMVMFLINAVYFYGEWKYEFEESATYDQP
                     FYLQGGGQIDVSMMHQEAEMKYYFSDEVAIAELPYGQGNFVMDVILPPIEQTAAELVS
                     TLDSEKWTQWQNGLYTAKVSLGMPRFEFSYEKTLNDMLIALGMNDLFDSGLADLSGIN
                     GTGGLFVSEVKHKTFISVDEKGTEAAAVTSVGVELTSVDPGVVYLTLDRPFVFIIREV
                     TTGAILFSGIVSNPVAE"
     gene            complement(45788..46669)
                     /locus_tag="PLK75_01630"
     CDS             complement(45788..46669)
                     /locus_tag="PLK75_01630"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOY37976.1"
                     /translation="MTIEIDRHSGFCPGVTNAIKMAELELEQNGVLYCLGDIVHNTIE
                     VERLKKKGLIIIDYNRFQNLKDATVLIRAHGEPPETYNTAKKNNILLIDASCKVVLNL
                     QRKIRQAEANGEKGIQIVIYGKKQHAEVNGLVGQTHSKAIVVSEMQDLNLIDFSKPVF
                     FFSQTTQSPEKYRDIIVEAKNRATRILGHHRNIEFLNSICPLVAKREPLIQKFATEHS
                     IVLFVSDPKSSNGKMLYNLCSETNPRSYFVSGPEDIKPEWFKADDHVGICGATSTPLW
                     LMEEVASCITNLAENIQ"
     gene            complement(46659..47375)
                     /gene="cmk"
                     /locus_tag="PLK75_01635"
     CDS             complement(46659..47375)
                     /gene="cmk"
                     /locus_tag="PLK75_01635"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOY37977.1"
                     /translation="MIKKKNIIIAIDGHSSCGKSTLARKLAEKLNYTYIDSGAMYRAV
                     TLYAIENELINASGFDENKLIKALPKIHIRFLKLGCNKPRTILNNIDVEEKIRSLEVS
                     AAVSKVSAVAEIRKHLVKLQRKLSENGGVVMDGRDIGTVVFPNAELKIFLTASAEVRA
                     KRRFKELTDNGIQVSFTEVLENVISRDYLDQSRTESPLRMADDAILLDNSDINAEETL
                     MVALAMVDVVMNKQLDINDN"
BASE COUNT        13768 a         9587 c         9668 g        14516 t
ORIGIN      
        1 cagaacgtcg tgagacagtt cggtccctat ctgtcgtggg cgtaggagat ttgagagggc
       61 ctgacactag tacgagagga ccgtgttgga caaaccactg gtgtacctgt tgtggcgcca
      121 gctgcattgc agggtagcta cgtttggaag agataagtgc tgaaagcatc taagtacgaa
      181 gctcgcctca agatgagatc tcccttaagg gtcgtcagag actatgacgt tgataggctg
      241 taggtgtaaa ggcagtaatg tcaaagccga gcagtactaa ttacccgaac gcttccagaa
      301 aaaaagatct ccatatctga aaatcttaaa tccgattaat taactacagc tacagacgtt
      361 aaaccccatt ccaggtggct atggcggcgg ggcaacacct cttcccatcc cgaacagaga
      421 agttaagccc gcctgcgccg atggtactgc gtaacagcgg aagagtaggt cgctgccgga
      481 tttcttttaa agcccttcat tcttttgaag ggctttttta tttattgcat ctctattgga
      541 taatcaactc cgttgaaact taccaccgct tgattgttgc agtcagtttc tccataatcg
      601 atttcaatca gaggcaaatc ttcaatcgtt acatgaagaa gccctgaccg aaaccacttg
      661 cagcaaacca acgcatttaa agattcttcc acattaattt cgtagtgtat gctatcggag
      721 ctgataccgg tttggcaccc agcaatcaaa tatacatcgt cgcagggctc ggttatagtg
      781 ctttcccctt caatccatgt cctttctgaa ttctgagtaa aataaatata tttgttttca
      841 ggagtagtaa tcttcccatt tgataccaca accgtatgca caataaagcc atcggtattt
      901 cttcctgtat tggtaactgt ttcgttacct tccactttgt gattgttctg atagaaatta
      961 tcgaaattta tcgtaagtac cgagttttcg ttcctgtaat aatcgcttgc catgataatg
     1021 attttccctc tgcggtaacg attgtcctga cacaggtaat tttctgaacc ccagtcaact
     1081 gtaatggttt taggccatgt gtgttgatcg aatggttcaa tggttatcac aggaaagccc
     1141 gttttgaatt gcttcaatga ccctagctgg ttgtcggcat ctcttgctgc attgtccgat
     1201 tcttcaaatg aatcggaaaa tatacctgca gcaattacgt attccctcat agcctgtacg
     1261 gtttgctcag cagtgggagg gttgtttctt tcgaaattgc atcctgccag caaccagagc
     1321 aacaacaaca gaagaataaa aaaactggtt ctcatgggtg ttattttttg cgggtgaata
     1381 ttttatatga aaaaggaggg tcatgttccg gatgctcttc cgccgatata caattccaat
     1441 cattgtaatt tatttcagga aagtaaacgt cggctgtata actccggtga acaagagtga
     1501 ggtacaattt ttcggcaagg ggtagaaact gccggtaaac cataccacca ccaatgatga
     1561 aattctcatc gcttgcattg caaagttttt ttacttcttc gatagaagaa gccactacta
     1621 cccctggaat gctgtcgttt tcgatatccg aaagaacaat acttcttcgg ttcggtagcg
     1681 gacgtaccgg taacgattca taggtttttt ttcccataat tatcgtgtgc ccggttgtta
     1741 ttgatttgaa cctcttaagg tcgcccgaaa tatgccagag taaatcgttg tttgctccga
     1801 tttcgaaatt tttccctata gcgacaatta ttgaaaatga cattgatatt aagttgttag
     1861 gttacacagc aatttgcccc tttatatgcg gatgcgggtt gtagttcgat aacaaaaaat
     1921 cttcgaattg aaaatcaaaa atacttgttc ggttgtttag gatttccata gtgggtagcg
     1981 ttctgggttc gcgctccagc tggagcttca cctgattaat gtgatttaaa tatatgtgtg
     2041 catctcccag agtgtgaata aactcgccgg ggcggtagcc gcatacctgt gccaccataa
     2101 gcgtaagcag cgcatacgaa gaaatattaa acggcactcc gataaaaata tctgcactgc
     2161 gctgatagag ctgacatgat aatttatcgc cggaaacata gaattggaac atggcatggc
     2221 agggcggaag cgccatgttt tccaaatcac ccacattcca tgcgcttaca atatggcgtc
     2281 gcgaatcggg attattttta agagaattta taagctctga tatctgatcg ataaccttgc
     2341 cttcggccga aggccacgat ctccactgat atccgtatat atgtccgagg tttccatttt
     2401 catcagccca ttcgtcccaa atactcactc cgtggtcttt gaggtattta atattggtat
     2461 cgccttgcaa aaaccatagc aattcgtaaa ttattgactt taggtgcagc ttttttgtgg
     2521 ttaacaatgg aaatccttcg cttaaatcaa agcgcatttg atagccaaat acactgatag
     2581 taccggtgcc tgttctgtcg tttttagtta cgccatgctg caacacatgg ctgagtaaat
     2641 caagataagc tttcatttgc tttagttaaa agtctaaata tagaccttat tataaaactt
     2701 tgccatgctc tgaaaatcaa tttttcaaaa agatatttac aattcaactc atctaatatt
     2761 tttgctatcc gagcttttct tgagattcgg attctaacgc attacattac cgataagtga
     2821 aaaatgaatg gtgatactga ttcacattca aaccgagtga ttaaaattat attcatttac
     2881 tgtctccgat tcccgcactg gttaatctgg actaaaatgc atcggggctt acatttcgtc
     2941 gccttcgtcc tggccgtatt cagaagtttt atctttacgc tttgtgaaat cgttgagctt
     3001 ataagtaatt gtaatattaa atgttggtga tttacgattc atataggtgt aggaatagaa
     3061 actttcagtt tctgaaataa attcatggtt catggtatta aaaatatctc tcaggttaaa
     3121 ggtaaccgaa aggctgcgat ccagaaagtc ctgacgcaat gctatgttta ccattccgct
     3181 gcctttggtt gtaccctgcg aggttattga attgccccta aggaagccac ttagctgaaa
     3241 agtggtaccg gtttttttaa ttttaaacgt attgttcatc ctggctcccc atgtaaatgt
     3301 gttattgctg tttacgttgc ctgaagcatc ggcaacaatt tcgtaatagt atgcctctcc
     3361 gctcagattc aggttccacc acttcgtaag attcacattc cccataagct ctcctccgag
     3421 cgacatgtct ttcccaatgt tttcccaggt actaatcatt atttcgctgt tatcggggct
     3481 aactttctta attctttcaa atttgttggt tgtgtacctg tagaagcctt caattgacac
     3541 gaaattgttc ccaaatcgtt tctggaggtt gagctcggca gagtttgtgc tttctggagc
     3601 cagcatagga ttcccctggc gaatattata tgcatcggtt acttctgggt aagggtcaag
     3661 gaagaaatcc caaggtcggt tgattcttcg gctgtaactg gtcattacct gcatttccca
     3721 gggtaactga tacgataagt gcagtgttgg gaagacatta aaagctttat acaccgaatc
     3781 gaaaggtaca attacggctg cctggtgtat tgttctgttt gtgtattccg agcgtagccc
     3841 gagcatatat ccgaattttc ccacttcatg cgaaaatgtc aggtatcccg attgaatatt
     3901 ctggtcgaag gagtagttat ttgtaaggct gtcaacccaa tcgtccacaa tcaaactctg
     3961 gtaatcatag ttgttgctgc tccatttttg tttaacctga tagccggctt cgagtttgcc
     4021 tttttcgaag agcggaaggg tataatcgat ctgaccccta agatttctag aaatcgaaaa
     4081 ttctcttgtc ctgtaactgt tagtgtcggt tgcactaaaa tattgcgtgt actgatcgtt
     4141 ttcgtcgttg tcgtcgtaat caaaataagt ataggcggtt agctcatgcc cttttttaag
     4201 gaatttatgc tgaaaattca aatctgctga gtagtatagt ccattatgct gccctgaatt
     4261 tacattattg taggaaatgc tgtcgctaac catgttatcg cgcaaccagt atgatgtaaa
     4321 atattcatca gcaccactat gcataccgtc ttttccaata tttcccgaaa gggttattac
     4381 gtcgtttgga gtaataaaga aatcgaagcc cccacggagt tttccatcct ctcggccacg
     4441 gttgttctgc gattctttat acacaaaaag ggtatcactg gcataagttc tctgttcgct
     4501 attacccgag cttttgttta accggttatt atagttgccg ccaacaaaaa aattaaattt
     4561 ctctgtacgc atattcacaa gaaaatctcc tcccagagta ttgaacgacc cataggagag
     4621 cgagaataat ccattgattc cggtacgttt gtctttttta agaattacat ttattattcc
     4681 gcccacgccg tctgggtcat acttggcaga ggggttagtg atgatttcga tgctttgtat
     4741 tgaaccggcc ggaatttgct gcaaagcatc gcttccctgc acaggactcg gcctcccgtc
     4801 gattaaaacg aggaaattcg agtttccccg aatgctaaca tttccgtcaa tatcggtttg
     4861 caccgatgga gtgttttcga gaacatccac agcggttcct ccggcagcac tcaggttttt
     4921 atcgacggta atcacctttc ggtcaatttt atattcaaca cttgggcctt cagcctctat
     4981 ctctacttca ttaagcattt caacagcagg gctgagttta attttcccaa gtgaaacatt
     5041 gttgttttca ggatttataa aaacatcgtt gactctgatt tttttatatc cgataaaatc
     5101 gatatcaaca taatacttcc cataacgcag cgatgttatc tgaaaaacac cttttttgtt
     5161 actaattcca ccacttacca gcgttgagtc gcgaagtgag taaatactga cattagcata
     5221 ttcaattggc tcattcgact tgttgtcgat tatagtacca aaaatgattc cgtcggcggg
     5281 agcatttcca cgtgggcctt cctgtgcatt cgatactgtg gaaaaaaaca aatgtaataa
     5341 agccacagta attaaaaagt acaacttcat acagcaacga tttataaaag tattagatga
     5401 aatttggaga aaaaggttta atttcagaat aaagtagttt ataacaggta ttattctgcc
     5461 agaattgtga ttatatcgtt tttcagctca ataacccctc cctgtatgtc gaacaatttt
     5521 tccgaattat cgggaagtac gattcgcacc ttgcctgcac taagaatcga aacaatcgga
     5581 gcatgatgat cgagcatctg aaaccgccct tttgcaccgg gaacttcaac aaaggttgct
     5641 tgtccttcga agagggtttt ttcgggtgta agtaatgtaa ccttcatttt taattcaatt
     5701 ctttagcagc agcaagcatt ttttctccct tttcgatgac ttcttcaatt ttcccaacca
     5761 gtaagaatgc attttcaggg tactgatcca cctcaccatc gagaatcatt ttgaaacctc
     5821 tgatagtgtc ttcaatcttt acaaaagcac ctttaatgtt tgtaaatgcc tcagccacat
     5881 ggaaaggttg cgagagaaaa cgctgaatac ggcgggcgcg gtgcacgata attttatcgt
     5941 cttccgaaag ctcatccatt cctaaaattg cgatgatatc ctgcaattcg gtatagcgct
     6001 gcaatatctg aattacacgt tgggcagtgt catagtgttc ttgtccaaca atttcgggag
     6061 tcaatatacg cgaagaagaa tcgagcgggt caacagcggg gtaaatccca agacctgcga
     6121 ttttacggct gagaacggtt gttgcgtcaa gatgtgcaaa tgttgttgcc ggagcggggt
     6181 cggtgaggtc gtcggcaggt acataaaccg cctgaaccga tgtgattgac ccgtgctttg
     6241 tactggtgat acgctcctgc atttcgccca tctcggttgc aagtgtcggc tggtatccca
     6301 cagctgatgg catacgtcca agcagagcag aaacttctga cccggcctga gtgaaacgga
     6361 aaatgttgtc gacgaagaac aaaatatctc tcccgccgga tttttcgtct ccgtcgcgga
     6421 agctttcagc aatggtaagt cccgatagtg caacgcgggc acgggcaccg ggaggctcat
     6481 tcatctggcc aaataccatg ctcagtttcg aatcttttat tgcttccgaa tcaactttcg
     6541 aaaggtccca gctaccatct tccatcgatt tgataaaatc gctgccatat tttacaatgt
     6601 tggctccaat catttcgcga agcaagtcgt taccttcacg agtcctttcg cctactccgg
     6661 caaaaaccga gaggccgttg tatccaacgg caatattgtt gattaactcc tgaatcagaa
     6721 ctgtttttcc tacaccggca ccaccaaata gtccgatttt tccacctttt gcatagggct
     6781 caataaggtc gataacttta atacccgtgt ataatacttc ggatgatgtg gtgagttgtt
     6841 caaactttgg ggggtcgcgg tgaatgggat acccttggcg gttgtcgagg gcttgcattc
     6901 catcgatttc ttttccaaca acattcatta atcgcccttt aacctgatcg cctaccggca
     6961 tttttattgt agtgccagta gctattgctt tcataccacg cgataatcca tcggtactat
     7021 ccatggcaat agctctgata gtgtgttcgc caatatgttg ctggcattct acaacaagaa
     7081 catttccgtc ttccctctga atttcgaggg ccgtgaaaat attgggcaaa tcgcttcctg
     7141 aacgatcgaa gcttatgtca acaacgggtc caattacctg tacaatttca ccaattatct
     7201 gctccatttt agtgtttttt atgaatgaaa caaatgtaaa ataaaaatat tgctttgtaa
     7261 ataccaaagg aatattaatt caattttaaa aacattcagg attgcctaaa agctccgcga
     7321 cttcaagctt aaaattaaat cacgaagaac aatttttttg ttttccttaa tggggatgct
     7381 gtttaggata ccgatagctt cggaaaaata cgcagtgatt gtctttagtg ttagttcttt
     7441 tattcccaat tggttgtata tatctgtaac tttcgatatt ttatcatcat tggcaagaaa
     7501 tatttcattc attgccgttg ttagttgctt tcgtgttgac ttgctgtcgg cgttcaaggc
     7561 agaaatcagc aaataagttt ttttatttgc aacaatatcg ccaccgatgt ttttaccaaa
     7621 gattttttca tcgccataca catcaagcaa atcatcctgc aattgaaatg ccatgcctaa
     7681 tttaagacca agatcgtaca ttttttcagc ctcatctgaa gttgctccgc ctaccaaaga
     7741 cccgatttta aggctgcatg ctattaatac tgctgtttta tacgtgatca tatgcagata
     7801 ttcatcaaca gtaacatcgg ctctctcttc aaagtccata tcaatctgtt gtccttcaca
     7861 tattttaaga gctgtatcgt tgaatatgtc gaggcactca ttcattttat atgagattct
     7921 cgaaatatgc ctgtatgcaa ttatcgacat tgcatctccc gaaagcagtg caatgtttgc
     7981 gttccagcgg gcatgcactg taggtattcc cctgcgcagc gatgctttgt ccataatgtc
     8041 atcatgtagc agggtgaaat tatgaaagat ctcaattgca gcagctgcat caatagcttt
     8101 gttgtagttg tcggaaaaca gctcacacga catgagggtt aaaatagggc gcaatctttt
     8161 accaccaagc gaaagagaat attctatggg tttgtagaga ttttcgggtg cctttgccca
     8221 ttcgtactgt tggaaatagg agttaattat tttatgaagc tcttcaaagg agtgcatcag
     8281 tgctgtagct taaattcaaa aaggccttgt tcgagaattc gtttcattcc ttcttcaagg
     8341 gagattaaat cacgattgag aacttcgcgc atccctttgt tgtcggggca gcgtcttgtc
     8401 atatctcctt ctgccagcgg aggaaggaat acaattttcg aactcgatcc ggtaagcttg
     8461 ataaccgtat ctgcaagttc aaggattggg gtttctgtat ctgttccaat gttaatcaca
     8521 tcgtttatgt acatgtcctt gcttgcaatc agcactgtag actcaatgtt atcatcaatg
     8581 tagcaaaatg tacgcgtttg cgatccgtca ccgtagatag tgatatcgtg attgtgcaat
     8641 gcgttgtaaa taaacttcga tataacaaaa tccttgctct gcttggggcc gtatgtattg
     8701 aaaaatctga aaattgtaaa attcaaaccg tattcacgtt gatacgaccg caggaatgct
     8761 tctccgaggt ttttcactat ggcatacgga agccgcgaat ttaaaggtgt agtgtgtgca
     8821 ttttgcggaa gttcaacagg ttctccgtac acttcggatg atgaagcgta aaatactctt
     8881 tttacccccg tgtttttact gattcttaat atgttttcca ggcctctgat gtctttaagt
     8941 acattgacag ggtgctcctg tgtgcgatga accccaacga ctgcggcata atgaaataca
     9001 taatggaatt gatacgatag catcacttca acaatatcgc gatattcgtt tacatcgcat
     9061 tttataaaac gaaggttgtc atatccggta ggtattttct caattctgcc cgttgataaa
     9121 tcatcgacaa caacgaccag gttttcattg tcggaggcca gtttttctgc taaagcacta
     9181 ccgataaaac cggctccacc ggtaatgagt atcttccttt tgaaggagtg acgtgatgcg
     9241 gttgtgtaca tgatatgatt tatttattga taattagttg atgttggttt ccgataatgt
     9301 ttcttcgtca taagcatcaa ttccgacaga acctattgtc tttctgtcgt aaaatttttc
     9361 taaagataat aataatgatg gtaataataa aagattgcaa atcattgatg caaataatgc
     9421 aattgaaaca agaattccca atgcttttgt tcccccgaat cctgaaacaa tgaaaatgct
     9481 gaagccaaga atcagtattg ttgcattgta cacaaggctg atgccgacct cctgaatggc
     9541 ttcgtttaca gcaactccga tttttttatt cagagcaatg tattcgtaac gaaatttgtt
     9601 tacaaaatga attgtgccat caactgaaat accaaatgct attccgaaaa ccataattgt
     9661 tgaaggcttg agtggaatgt tgaatgctcc cattatagct gctgtcagta tcattggtat
     9721 catgttaggg ataaggccaa gaagtgtcat acgtacagag cggaacatcc agtaaagcaa
     9781 aacggcaatt acaataaaag ccaacgaaag actcgaaaac aaattcttag taaggtaggt
     9841 ggtgcctgtg aaatatagga ttgtactacc cgtgataacc gtatgatatt tttccggtgg
     9901 aaaaatttgg tctacctcct ttttaataaa aggtatcaat actttcatct tttttgttcc
     9961 cacatcttca acattcaggc ttacgcgggt gattgttttt gatgaatcga taaatgaagc
    10021 aataaactgg ttattgaaat cgaatttttg aggaagacgt ttaaaaattg tctcgtaggt
    10081 tttcgggtta tccggtaacc ggtaagcaac acttttccct tgcgaatagg cttgattcag
    10141 aaattttaga gcatctgcaa ccgaaacgga tctcgataac tctttgtgtt ttaataagga
    10201 tatttgaaga gaatcgattt tttgaatctg agtaataaac tgatttcccg acaaagtgtc
    10261 ctgagtttta atcataattt ccagcggact aactccgtga aaacatgatt ccagatattt
    10321 aaggtcaaca tacatttttt tcttatgagg tacgtcgtcc attaagtagc ctgtctgcct
    10381 gatgtttatt atacccagca cactcagcag cattacggat gcgataataa tatagagtgg
    10441 tttacgatgg gaattggtaa ttaattccgc tttacgaata acccaagtag ttattttgtt
    10501 gtcaaggtgc cgtgtgtatt tttcggatgg cacgggcaag tagctggaaa ctacaggaat
    10561 tactattaac gacaaaacaa agctgaacaa tactcccaaa gatgcaatta acccgaactc
    10621 ccaaagaatg cgactatttg taatgatgaa agtcgcaaac ccaacagctg ttgtaaggtt
    10681 cgagagaaat actgcagtgc ctttacgccg gatagccctt tgtaaggcaa gaattttgtt
    10741 tttatttgcc ctgacctcgg tatgatactt attcaataaa tatataatgt tgggaatccc
    10801 gattacaatg agcaaaggtg gaatcattga agtaagtacg gtaattttat agtgaaacaa
    10861 tcccatcaga cccgttgccc aaacaacggc agtaccgaca accactatcg aaattaatat
    10921 gattttaata ttgcggaaaa ataagaacag gataatcgca gaaattaatg cagcaagtac
    10981 aatgaaaatt ttaatttcgc tgcgtatcat tgtcatcagt ttggtcctaa tgaaaggatg
    11041 gccagaaatg aataccggga tattatttgc agttgaatat tcttcaacgg ttttttcaat
    11101 ttgctttacc gcaggaatac gcaatgggct atcgagaatt tcgtttttaa gtgaaaccga
    11161 tagcagaaat acatgttttt cgtgattata cagtaatccg ttgtaaaaag gcaggttcat
    11221 aaaaacagtt ttcaggctgt cgagttgctg ttgtgtgtag gtagagtcgg aaaacagcgg
    11281 gtatatttcg aattgtcttt tttttgaatc accggtttca actgtaattt gacgtatgtt
    11341 aacagcctgt ggtatgctta atactgattt tacattgggg atttttttca gatcgttgca
    11401 taaacgtgtg aaattattga agtgacctgc ttcgaaaaat ttttcatcct tgataccaat
    11461 aaatattgca ttggcctctt ctccaaaagt tgctttaaat tctttgttat caagataaat
    11521 tgggtccttt tcagaaagaa gatttgcata gtggtaatcc atcctcactc ttgtggtttg
    11581 ccaaatcata acacctgtaa tagcagtaat tgctataagc aggggtaatc ggttacgaag
    11641 gataaatccg gctatttttg tccaaaacaa agcatagaaa tttgtgcaaa attagccttt
    11701 acttatcatt ttccaaaagt ttttaaatct cggaatatct tgcttaaatg aatcacagca
    11761 ccaccagtat taatgccaca attccacaga tcctgtttcg gttttattgg atgtttctaa
    11821 atcagaagga ttttcgaaaa tcgatatacg ctttgagcat tttttgaata tctgtaatta
    11881 aattttgtgt ttcgagcaac attgcaataa ccagcatact gcttcgggtc ggatcttctt
    11941 tactgcgaat acgtttgatt tgttcgctac gcacgttttc tattactgaa ttaatatttc
    12001 cataggttac tgaaatcgat tcgaattcgc tttcgctgaa cgaatttttc tgaataaggt
    12061 taattatttc tgtgaaataa aaacttatag gattggaaat ttgtgttagt ccttcaatct
    12121 gtgcaggggt gagttcttta tggttattat cgacgtgccg gaatgcggga tcaatgattc
    12181 gtttcaggca attcgccgat tcgttaaggt aatcaatcat ttgaacatag aaaattccgg
    12241 aattttcgaa attaatgtta gagcagctaa gcgttggata gatatcattt ttatattgct
    12301 gtactttctc ctcaatttca gatgatagcc tgaatctttt cttcagtttt ttatgcttga
    12361 atttacaaac cgcatcaatt gcatcggtgt agcaattaga aatgtcacct gttatttcct
    12421 gcagttttag ttttgtttct tcgatgaatg agttcgagat ggtctgactt ttttctgact
    12481 cggtttcttc gcgttttttg tgaataacat ttgaccgtat aaagaagaat acagctactg
    12541 ctaccaaagc aattacagct attaatccgc cgaaataaac aatcaatagt attatagaag
    12601 aaagtaagaa ggcaagcagt gctgtgataa accaacctcc aataaccgat attacaccgg
    12661 ttatccggta cacagcactt tcgcgacccc acgatttgtc ggcaaacgaa gtggccatag
    12721 caaccataaa tgttacatac gttgtggaaa gtggaagttt caacgatgtg ccaagagcaa
    12781 taagaatact tgccacaata aggctgacag atgccctaag catgtcgaaa gcaggacgtt
    12841 gttcaatttc agcagaactg tatactctgg gttcaaattg tttagcgata cgctttttta
    12901 atccagatgg tacaatcttg ccaaaggttt gcgctgaggt tactcccatt ctcacaattt
    12961 cgcgggcaat caggctgctg ccgaaacgtt cgtgcccttc actctgacgg ctcaggtcaa
    13021 cagaagtctt tatgaccgac cttgattttt tcgagagcca aattgccagc accatgatta
    13081 ccccggctgc tataagaata aatgtattta cacgaaccgg gcccgaaagg gctcccattg
    13141 tgaagctttc cggatttgca acccccgatt cagtgaaaaa ctgatatgac gaccagcccg
    13201 ccataggcac tccgataaag ttcaccagat cgtttccggc aaatgccatg gccagtgtta
    13261 tcgtgcctgt aagcactatg attttcaaaa tattgatatt gaaaagaagg tataagattt
    13321 cgaatagtat ggtaaacccc acaaagctat aaaggagaat ttccagagtg tgagtactga
    13381 taaacgtatt aatttcttca ttcataaacg atgcgccatg caatccctta ataagcataa
    13441 aatacacgat tactgttatg gcgattccgc catagatagc accgaaatat ttaatttttg
    13501 cttcatagtt gaacgaaaaa atcaatctgg ctaaccattg tataaaagcc ccgacagtga
    13561 atgcaattac tatcgaaata agaatgccgg taattattgc cagtgctttc gaagtattga
    13621 tgtattcact tatcgataat gcatcgggtt gaatgttcat tttaataagc gccatgctaa
    13681 cggaagcccc caatagtgca aatacaagag ataccgtggt tgatgtgggt agcccaaggg
    13741 tgttgtaagc atcgagcagt aacacatcgg tcagcattac cgccagaaaa ataaacatca
    13801 cttcgttgaa ataaaaattg tttgggttga atattccttt tcgtgcaact tccatcattc
    13861 cgctcgaaaa tgttgctcca ataagtacac cgatacttac taaaacaaac aatattttaa
    13921 ttttggaggc tttcgagcca atggcggggt taagaaaatt tactgcatca ttagcgacac
    13981 caacggttaa atcggttatt gccaaaatac acaatacaac gagaattacc tgaaaaacca
    14041 acataacaaa taaaattatt tactgtgcaa gaatacaatc cgactctaat tttaaggtta
    14101 cgttatacag caccaatatt aaattaatgt taagttttta tgggaaaact tttcctcaaa
    14161 acgattttcg aaaattgata tatgctttga gcattttttg catatcccca agcaaattct
    14221 gacactgctg aagaatattg attactaaaa tactggaacg ggttgggtct tctttcgaac
    14281 ggatacgttt tatttgttcg cttctcactt tttcaataat attttcgata ttggcactgg
    14341 ttgcagaaag ttcagcgaaa ttttccagag tattgttctg cagcatgttg ttgtactcca
    14401 caaagtagaa ctccatagga ctgagtatac gttttaaccc ttctatttgt ttttcggtaa
    14461 gctctttatg attgttgctt acatgttcaa aggctggctc tgttattttt acaagaacac
    14521 ctgacgattc ccttaagaag tcgatcatct gaacgtagta aactccggaa ttttcgaaac
    14581 tgatacctgc tttactcagg gttgcgtata tgtcgttttt ataggctttt gtcatctggt
    14641 cgatttcttt cgaaagcttg tttcttttct ttagcttttt aagtttgaaa tcgcatactc
    14701 cgtttataga atcaatgaga cagcctgtaa ttaattcaga aagttcacaa acacgaagtt
    14761 tggtttcttc ggtaaatgaa tttgaaaccg tatgttgata ttcgagttct ttttcttcgt
    14821 tttttcggtg caccaaattt gacttgatga agaaaaacgc tgttaataaa ataaggataa
    14881 ttatggcaac aaggcctccg aaataaaccg ccacaagcag aattcctgca agaacgaatg
    14941 aaatgagagc ggttataaac cagcctccta ttaccgaaag aactccggtt attcgataaa
    15001 ctgcactttc gcgcccccag gccttatcgg aaaacgaagt tgccatcgac accataaaag
    15061 ttacatacgt ggtcgacaag ggtaatttca tcgaagtccc tagcgcgatg agaatacttg
    15121 ctattactag gctgactgat gccctgagca tgtcaaaagc agggcgttct tttgagtcag
    15181 ccgcactata aacctttggt tcaaattgct tttcaatttt tctgaccagt attgccggca
    15241 tcactttgtt tacagcctgc cctgttgaaa cggccattct aactatttgc cgggcaagca
    15301 gactgcttcc gaagcgctcg tgaccttcgg tttgcctgct aagatttaca gtggttttaa
    15361 taacatttcg tgcttttttc gaaaaccata aagccagtac catgattaaa ccggcagcaa
    15421 tcaggtataa cacgtttgcc ttcattggtt ccgataaccc ggtcatagtg aaagagctag
    15481 gatcgttgca acccgattct cggaaagtgc ttaatgaatc gagccctgca aggggaacgc
    15541 caataaaatt cacaaggtcg ttccctgcaa aagccattgc cagcgataag gttcctgcaa
    15601 gaacaacaac tttgagaatg ttgattttaa acaacaaata tagaatttcg aaaactacac
    15661 tgcttgcaac aaagaaataa agcagaaaca accatgtgtt tttatttaac cattcgttga
    15721 gttcaggcgt catgaacgaa gacccgccaa gcccttttat tatcatgaaa tatagtatga
    15781 tagttattga aataccccca aatattgccc ccagatactt cattctcggt tcgtagttaa
    15841 acgaaaacaa aactctggca atccattgtg caatagcacc aatagtaaat gccacaacta
    15901 tcgaaaataa aataccgaaa atgattgcca ttgcttttga agtattaatg tattccgata
    15961 tattcaaagc gtccggctga gtattcattt ttataagtgc catggcaata gaaccgccaa
    16021 gaagagcaaa tactagcgaa actgttgttg aagttggcag gcccagggta ttgaaagcgt
    16081 caagtaaaat tacgtttgtg agcattaccg aaaggaaaat aaacatcacc tcggttaagt
    16141 agaaattgtc gggattaaaa atacctttgc gggcaacttc catcattccg ttcgagaaag
    16201 tggcgcccag aactactccg acacttacca gcagaaaaag tagcttagta cttgctgtca
    16261 ttgcaccgac tgcaggattt aaaaaattta cggcatcgtt actcacgcca acaaccaaac
    16321 tggctactgc cagtaaaatc aacacaacga gaattacata caacaaaacc atgaccaaat
    16381 aatttgtcga taaaactatt caattaacaa ttccttaacg ttacgttaat gttaagttta
    16441 cctgcaaaat aaatctgaag tatattgatt aattatgaaa cacttatcgg cgcagttcga
    16501 aattctgtcc aagatatacc tttcggacct gttcgtcttc cgaaagctct tcggctgttc
    16561 ccgacttcag tatgctccct tcaaacagga ggtaagccct gtcggtaatt gaaagcgttt
    16621 cttgaacatt atgatccgta ataagtacgc ctatgtttct gtttttcaag tgcataacga
    16681 tttgctgaat atcttcaacc gcaatggggt cgactcctgc aaaaggctcg tcaaggagta
    16741 taaatttcgg gtcaattgcc agagcccggg ctatttcggt gcgtctgcgt tctccgcccg
    16801 aaagttggta ccccttgctt ttacgtattt ttgttaaacc aaactcattc aataacctta
    16861 cagttttttc tttttgttcc cgttttgaga gtttactcat ctcaagtatc gaaagaatat
    16921 tgtcttcaac gctcattttc ctgaaaaccg aagcctcctg agcaagatac ccaaggccaa
    16981 gccgggctct tttatataca gggtctcctg taatttcttt atcgtcaaca aaaatttttc
    17041 cttcgtaagg tcttattaag ccaacgatca tgtaaaaagt tgtcgtcttt cctgcaccat
    17101 tgggcccgag caatccgacg atttcaccct gattaacttc aaccgaaaca tctttaacaa
    17161 ccatccgttt gccgtacctt ttatagagat tttcggtgta tagtttcatg atgcacagtg
    17221 tttttttgat gttgcaaata tagtgtattg tttattatac ccaaatcccc atatgatttc
    17281 ctgattcggg ttggattcag ggagattcaa atcggtataa aattgaagtt gaaaatctta
    17341 ataaatactt aatcttatga attgcgaaat atcaactgcg gtttttgatt atttttaccg
    17401 ataaaataac agcaatgctc cgtttaatat ttgcctttct atttttatcg gttgccgtac
    17461 caattgttgc acaaaaacaa tggacactta aggattgtat cgattacgcg ctggagaaca
    17521 atctggaaat cagtcagcaa tcgctctata ttgatatgta cagaaacaat ctgctgtcgt
    17581 cgaaactttc ggtattacct tcgttaaacg ccggagtgaa tcagaatttt tcgtttgggc
    17641 gcagtgttga ttcctatacc aatgagttct cagaagagaa tatttcttct tcaaatgcaa
    17701 gtatttcaag ctcagtaacc ttgttcaacg gtttacagca gtacaacact attcgtaaaa
    17761 gcaaggccga tctcgaagcc ggattggccg acatggaaac agcgcgaaat aacctttcac
    17821 tgagtgtaac ctcggcctat ctgcaaatat tatattctgt agaaatgctt gaaacagcta
    17881 cccttcagat ggaagtaacc cggtctcagc tcgaacgaac catcaagctt gtgaatgcag
    17941 gtagtttgcc tgtacagagc caactggaag ttgaagctca gcttgcatcc gaagaaatga
    18001 gcatggttac cgctcaaaat cagctggata tggctttgct gaacatggta cagcttctgg
    18061 agctaaatga tgtagcagat ttctcgattg ttaagcccga tttctcaggt gtaatcggcc
    18121 accctgcatt gatgacggtt gacgaaatat ttaatgaagc ggttatgaat atgccggaaa
    18181 ttaagagtgc ggatatgaaa ctgaaaagct cagaatatca attggctata gcacggggct
    18241 cgagaagccc gcaattatct gtttcggcga gctacggaac gggttattca aatgcccgta
    18301 aaatgattga tgatatcaca cccgcttcac cggttcttac aggttatgcc gttgatgctt
    18361 atggtaacta cagtgatgtt taccaataca gttttgatta ttcatatatt acgagaccct
    18421 ttagcgatca gatccgcgac aatgccagtg catccgttag tttcgggctg agcataccca
    18481 tttttaatgg atggtacaca aacaccagaa taagtaatgc taaaattgat gttttgcagg
    18541 cgcaaatgca gttcagtatt gcagaacgcc aactatataa agtaattcaa caggcccatt
    18601 ccgacgtcag agcagccctg aaaaaatatg atgctgcagt aaaagcttac catgccagtg
    18661 aaacttcatt ccagtacacc cagcaaaagt tcgatttagg tctggttacc tcactcgatt
    18721 ttaatacagc aaaaaccaac cttaataaac ttaagtcgga aatgattaat gccaaatatg
    18781 atatgatttt caaaatgaaa atactagatt tctacagggg tgtcgaaata aaattgtaaa
    18841 aattttacat aatagcaata taactacttt ttaatcagtg ttgcaaatga aaacgaaaag
    18901 aaagaaatta taccgttggc tgattcttgg cctgatagtt ctgatagtat tattgattgt
    18961 tgcacgcaaa gcgggttgga tgggtggaga agaaattaat aaagtaagcg tcgataaggt
    19021 ggctaagagg actattgttg aaacagtaac cgccaatggt aagatacagc cggaaatgga
    19081 agtgaaaatc agtccggatg tttcgggcga aattgtggag cttgatgtca aagagggtca
    19141 gcaagttaaa aagggacagt ttctgttaaa gattaaaccg gacttgtacg aatcggctgt
    19201 cgatagaagt attgctgcac tgaacggtgc aaaggcaaac ctttcgaata gcagagcaag
    19261 gctggagcaa acacaggcac agttcaatca aactgaactg gctttcaatc gtaatcaaaa
    19321 attattcgat cagggggcta tatctcaggc tgaatatgag aattcacttg catcttatca
    19381 aatgggtatg gccgacctga atgcagcgcg cgaaaatgtg aaagctgcag aatttactgt
    19441 gatgagtgct gaggctactg taaaggaagc aaacgagaac ctgtacaaaa catcaattta
    19501 tgctcctgtt gacggtacaa tttcgttact aaatgttgaa ttgggcgaaa gggtagtagg
    19561 aaccgaaatg atggcaggca ccgaattgct tcgtattgca aacctcgatg taatggaagt
    19621 tacggttgat gtaaacgaaa atgatattgt aaaaattagc atcaacgata cagccgatat
    19681 cgaagtggat gcatacctga ataaaaaatt cctcggtgtg gtaaccgaaa tagcaaactc
    19741 agcaacctca acagggttgt cggctgatca ggtgacaaat ttcaaggtaa aaattcgaat
    19801 tcttcaaacc tcgtatgccg atttaattaa tgcaaacaat aaatttcctt tccgaccggg
    19861 aatgtctgct actgtcgata taagaaccaa tgcagcaatt aatgttattt gtgttccact
    19921 tatggctgta acaacgcgcc ccgattcagc atcagcatta caggtaaaac cgggtgcaaa
    19981 agatgacgat gataggtcaa ccagcgatat tacacttaag gaggttgttt ttgtttataa
    20041 agatggtaaa gcggtaatga aaaatgtagt aaccggtctt caggactcct tttttataga
    20101 aataaaagat ggcctaaccg agaatgaaga ggtgatttcg gctccgaata gtctgatatc
    20161 gaagatgctc aaagatggca tgactgtaac aaaagttgat aaaaacgcat tattcagcga
    20221 aacaaaaccc aaataaatgc cggtaatttt gtccattgaa acatctgcca gtacttgttc
    20281 tgtggccata gcaaacgatg gcagaaacat ttctgtaaag gaaaaccatg tcgataaatc
    20341 gcatgcttca atattaaatg tattaatcag ggaggcagtc gctgaatccg gaattccgat
    20401 ggggaagatt gatgctgttg ccgttagcaa aggtccgggc tcatatacag gtctccgtat
    20461 tggggtttca tcagcgaaag gaatttgtta tgcacttaat aaaccgctca ttgcagtaga
    20521 tacattgaag ataatggcaa taatggcatc tcaggttttt ttgcaaaaac tggatgaaga
    20581 gttgttgata tgcccaatga ttgatgcccg gcgcgatgaa gtgtatactg cattgtttaa
    20641 ttcccggact gaaaaactta atgaaactac tgcagtaata atcagtccgg attttttcgg
    20701 cgatattgat ccagaaattg ccattctgtg ctgcgggagt ggcgccccga agtgcaacga
    20761 aattgtaaaa cgaaagaacg tggttttttc cgacagcatt ttcccgtcgg cagagtttat
    20821 gtgtcaacct gcgctggact tatattcaaa agcatgcttc gaagatgttg cttattttga
    20881 accatattac ctcaaagact ttattgctac agttccaaag aataaaattc cagggataat
    20941 agccagataa tccataaaaa ttttatttag tgtattgatt tttcggaaaa atgtttacat
    21001 ttgcaacccg atttaacaag ggcaacaaaa atattagtac aatgaaagaa aatgtgcatc
    21061 ctaaaaatta ccgactggta gcgttcaaag atatgtcgaa cgaacaagtg tttattaccc
    21121 gttctgctgc gcagacaaaa gaaactatcg atatcgacgg tgttcagtac ccgctgttta
    21181 aactggagat ttcaaacaga tcgcatccgt tttataccgg aaaaatgaag cttgttgata
    21241 ccgcaggacg tgttgataag tatcgcagtc ggtaccagaa acatatcgac aaaaacaatc
    21301 agaatagcta agctctgggt tttaaaatat tttcagcgat gccctgttgt aatgcagggc
    21361 atttttttta tttttatagg ttgtatttat aataatctta catgaaaaat tacattcttt
    21421 ccgatgcagg cttgcataag aatttacttc caataacatt taccagacct gtgtccgaaa
    21481 ttagatcggg aattctgaca aatactcagc gttggcaaca ttttttggga gaaaagtgct
    21541 ccttcctaac cgaaatctat cttcagaaaa aattcccggc gagcacaggt caagcaaata
    21601 tctggattaa tggcggagtg gttgctgata aacacattgc tgaacgggta ctcaatctgc
    21661 agcaaggatc actaatgtgc aatggtgttc ttatagctgc agtttgtgtt ggaactccac
    21721 cggttatttt cgacaattac ccagagcctg ttcaggtgta cgatgggaat gtgattgtaa
    21781 tacaacaggt atatgatatt tttgggttag ccgcgaagtg tattgatatt gattttgaat
    21841 tactcgctgg aggaagagta tcgaataagc tgagtaaaac caatacggtt ataggagata
    21901 acccggtgtt tctcgaggaa ggggctattg ccgaagcttc ggtttttaat accacaaacg
    21961 gacccattta tcttggaaag cactgcgaag ttatggaggg gagcatgatt cgtggccctt
    22021 tctgcttgct tgaccattcc gttttaaaaa tgggagctaa aatttatggg cctaccatta
    22081 taggacccca ttcaaaaata ggaggagaat tgaacaatgt ggtgatttta gggttatcga
    22141 ataaagcaca tgatggtttt ctcggaaata gtgtaatagg agaatggtgt aatttgggtg
    22201 ccgatactaa taattcgaac ctgaagaata attatgccat ggtgaaactc tggaattacg
    22261 agaaaaaaag ttttttgaat acaggattgc aattttgtgg gttaattatg ggagatcatt
    22321 ccaaatgcgg aataaataca atgttcaaca ccggaactgt ggtaggtgtg agtgcaaata
    22381 tttttggttc aggattcccg cgaaactaca ttccatcctt tagctgggga ggtgcttcag
    22441 gatttgtaca gtttggatat gaaaaagcca ttgaagttgc cggattggtt atgaaacgaa
    22501 gaaatatgga gttggataat accgagaaag aaatatttaa gtacctgttc gacaataatg
    22561 atcacaaatt ctgaccccgt tgttgtgtgg cataacaatt gccatctaag gtaattattt
    22621 aggttttatt gaattttgtc attttggcag ttaattatga atgaattaaa cgatttttta
    22681 ataaacgcac tttcgctatc gggtatagaa aacgatggtg aagttatccc aattttaagt
    22741 attgatgatg ataaatccga agaaaatgat tcggattcgt acccggaaaa attgccaata
    22801 ctccccctgc ggaatactgt gttgtttcct ggtgtggtaa tgcccctcac agtgggcaga
    22861 agcaaatcgt ttcaactggt aaaagaggtg tacgcttcaa ataaaatgtt gggcacagtt
    22921 tcgcagttgg aagcttctat tgaagacccc gaaattgacg atttgcacag tactgggact
    22981 cttgcaaaaa ttttgaagat tatcgaaatg cccgatgaaa ctatttcggt tattatacag
    23041 ggtaaaaagc ggattgaaat caacgaaata gttgaaaagg aaccttactt tatgggatat
    23101 tataaaattc ttcccgacct taaaccggct ggctccgaca aagaattttc ggcaattgta
    23161 agtactttga aagacctgtc gcttaaaatc attaagctca gcaattcaat accaaaagaa
    23221 gtggcttttg ctattaaaaa tatcgataac tcttcgtttc tgattaattt tatttgctct
    23281 aacagcgata tacagcctat cgacaaacaa catctgctcg aaaccgatga cctaaaagag
    23341 agggctattc acctgatgga acttctaacg aggcaaatac aaatgcttga actcaaaaac
    23401 gacattcagg caaaagttaa aaaagacctc gaccagcaac aaaaggagta tttgctgcat
    23461 cagcagatga aaaccataca ggaagagctc ggagggaatc ccatcgatca ggaaataaaa
    23521 gaaatgacag agcaggctaa gaaacaaaaa tggaacgatg aagttaaaac gcatttcgaa
    23581 aaggagctca gtaaactgca aaacctgaat cctgccgctg ctgaattttc ggttcagata
    23641 aattaccttc acaccctgct cgatttgcca tggggagagt atactgccga taacttcgat
    23701 cttaaaaatg ctgctactgt tcttgatcag gatcattatg gcctcgaaga agtgaaaaaa
    23761 cgtattttgg aacatcttgc cgtgcttaaa ctgaaaggag atcttaaatc tcccattctt
    23821 tgtcttgtag gccctcccgg agttggtaaa acttctcttg gcaaatcgat agcacgtgct
    23881 cttggcagaa aatatgcacg gatgtcgctt ggtgggcttc atgacgaatc cgaaatcaga
    23941 ggacatcgca aaacttatat aggggctatg ccgggacgta tcatttataa cctgaaaaaa
    24001 gtgaaatcgt cgaatcctgt tttcgttctt gatgaaattg ataaagttgg caaagatttt
    24061 aggggcgacc ccgcttcagc attgctcgaa gtgcttgatc ctgaacagaa ctccgaattc
    24121 catgataact ttatcgaaat tgagtacgac ctttcgaaag taatgtttat tgcaactgcc
    24181 aacactacca atacaatcag cccggcactc ctcgacagaa tggaactcat tgatgtaaac
    24241 ggctacattg tgaatgaaaa aatcgaaatt gctcaaaaac atctgatacc aaagcaactc
    24301 gaagcgcatg gtttaacaaa agaccatgtc tgtttcgata ataaaacact tgaatatatt
    24361 atagaaaact atacccgtga atcgggtgtg cgcaccctcg acaaaacgct ggcaaaactt
    24421 atcaggcaaa ttgccctgaa agttgctgtc gaagaaacct acaacccgaa aatggatgcc
    24481 caggctgttg aaaatttgct tggcagtaaa aaatacttta aagaaaaata tcaggataat
    24541 gaaactgccg gtgtggttac cggattggcg tggacagcca ctggaggcga aatactattt
    24601 attgaatcca gcctgtcggt tggtaaagga accgtaacac ttaccgggaa tctgggcgaa
    24661 gtaatgaaag agtcggcaat tatagcccat gaatatgtta aagctcattc agaacttctt
    24721 gatatacctt caggtgtgtt cgaaaaatgg aatattcatg ttcatgtacc cgaaggagcg
    24781 attccaaaag atggaccttc agccggagtt gcaatggctg tttcgattgc atcggcttac
    24841 acacagcgta aagtgaaaaa gagcattgca atgacaggtg aaatcactct cagaggtaag
    24901 gtattacctg taggaggtgt taaagaaaaa attcttgcgg ctaagcgtgc cgggatttca
    24961 gctattttac tttcaaatga gaataaaaaa gacgttgaag aaataaaacc gatgtatatc
    25021 gaaggcctgc gcttcattta tgtcgataca atactggatg tttttaaagc tgcccttact
    25081 ctgcaaaagg ttaagaaccc caaaaacatc gtataagcgg gttatctttc gaaccgaagt
    25141 ggcattgctg cctgaacttc gataattttt cctttatcaa acactttctt cccattcacc
    25201 caggtgctta caatgccatg tgaaaaagtt tgattttcga gaggagacca tccacatttg
    25261 tacagaatat ttccggggct gacagttgtt ggtgcatttg ggtttaccag cacgaggtca
    25321 gcataatacg actccctgat atatcctctt ttttgaatct gaaacaatgc tgcaggttta
    25381 tggcacatgt aatcagcaac ttgctgatat gaaaaattgt gttttgcagc catttcaagc
    25441 attacaacca gtgaatgctg caccataggg gctcctgaag ggcattgtga gtagtggctt
    25501 agcttctcgg caatggtatg aggggcatga tcggttgcta caatatcgat gtaccccgaa
    25561 ttcaggcctt gtattaatga aagtctgtcg ttttctgttt taatcgatgg attccatttt
    25621 attcgcgacc ccagtctgtt ataatcattt ttgctgaacc acaaatgatg tacacatact
    25681 tcaccggtaa tcgttttttt attcagtggt atttgctgca ttagcgagat ttctttttct
    25741 gtgctcaggt gcagaatgtg cagcctggca ttgtgctttg tagctagctc aacggctctt
    25801 gccgaagaag cgtaacaagc ttcctcgcta cgtatatcag catgatgatg catggggata
    25861 ttgtcgccgt ataaggcttt gtattttgca aggttccggc tgataatact gtcttcttcg
    25921 caatgtacgg caaccagcac aggggcatga gcaaaaacag ccgaaactgc ctcgttggag
    25981 tgcaccaaca tattacccgt cgacgatccc ataaacattt taataccgca aacaatcaag
    26041 ggatcagttt tttcgatttc agcggtatta tcgttggtag caccaagata aaacgagtag
    26101 ttaatcaacg agctgtgtgc agcaataatg tgcttattgt gcagggcttc catggtagtg
    26161 gtttgaggga tggtgttggg catatccatg aacgatgtta taccccctgc tgctgcagct
    26221 cgggattccg aaaaaatgtc acctttcgta gttaacccgg ggtctctgaa gtgaacctga
    26281 tcatcgataa caccggggaa gagtagtaga cctgttgcat caattacctc gtcaaaatga
    26341 ccggactctt ctttgcggct gattttaaga attacatcat cttgtataat tacattggca
    26401 acataaattt caccttcgtt taccagactt gcattcttaa ttaatgtctt catagtttct
    26461 ttattaatta tagtgggtca acatctatcg atatatctac acctttgtac tctgggtgct
    26521 ttttaattgc ttctatacta cttaaaataa agtcctttgt ttttctcact gaaattcctg
    26581 cttcgaaacg gataataaca ttcataataa accatttctg tactttgttg acgatgggct
    26641 cgtgaggacc ctgaacgcgg tttgggagca attgttccag tgtgttctta agagaaatgg
    26701 cagcggaatt gagagttgag taattgcgat gtcttatttt taaattgatg aatctgtaaa
    26761 atggtggata tcggtaagta tttctttctg ttagctcatg ccgggcaaac ccgtcataat
    26821 ttccttcaac cacatattta attacatcat gctcgggcat tgatgtttgt atgatgactt
    26881 tgcccctttt ttctcttcgc cctgccctgc cgctcacctg cgacattagc tgataactgc
    26941 gctcaaacgc tctgaaatct ggaaaattca gcatattgtc cgcattgagg atgcccacta
    27001 tcgacacatt gtcaaaatcg agccccttcg aaatcatttg cgttccaatc agaatatcaa
    27061 tatctctgtt ttcgaagtca gcaattaatt tttcgtattt tgcgcgtgtg ctgacggagt
    27121 catagtcaag gcgttcaatt cttgcctccg gaaaaaatat acgggcttca ctttcgacca
    27181 tttccgtgcc aaagcccatg gtttttatgg atgtactttg acaatcggga cagttcctga
    27241 ctaatcctgt gctatagcca cagtaatgac aaagcatctg ctgacggcgc ttatgaaatg
    27301 tgaggcttac gttacagttc ttacaggttg ctacccagcc gcattcatta cattcaatgt
    27361 aaggagaaaa gccccttctg ttctgaaaaa gtattacctg ttcactttta tggagtgctc
    27421 ctttaacttc ctcaataagc aatggatgaa aatgcaactg catcgatttt ctttttctag
    27481 cctgcagcgt atccacaatt acaatccgtg gcatttccac attcccgtag cgcgttgaaa
    27541 gtaaagcaag tccatattta ccgcttatcg cattaaaata actttcaacg gaaggagttg
    27601 cagatgcaag caatactttt gccccgcatt tttcagccat tacaactgct atatcacgag
    27661 cgttgtatcg tggtgaattg tctgattgct tataggtgtt ctcatgttct tcgtccacaa
    27721 ttacaatgcc cgggtttagc agtggtaaaa ataccgacga ccttacccca ataattactt
    27781 taagttgacc atcctgaatt tttttccaga cttcaacccg ctccgaatcg ctgtatttac
    27841 tgtgatatac cccgacaatg ttaccaaaaa ccgattttat acgatgcact atttgggtag
    27901 taatggcaat ttcaggtagc agatacaggg ctgtgtggtt gtttctgaca gcttcatcaa
    27961 taagatgaag atagatttcg gttttccccg aggaatttac tccatgcaac aggcagacct
    28021 tgttttcatt aaaaacagcc ttgatttctt tcaaacagtt gagttgtacc gcacttaact
    28081 cttttaacaa acttgtgttt tcggtctgtt gaaatctgct tgtttctttc agaacgaagg
    28141 aaatgatgtt ttttttcgag agagcaatta ctgtctgcgc atcaaggcct gttgatgtta
    28201 aaacttcgga gagctctgcc tggaaagaac cgtactcttc accggcttgt tcaatgagaa
    28261 aggcaagcat ttgcgcctgc tttggtgcgc gtttcagtga gtcgagcacg ctgtgggctt
    28321 gttcggcgga aataaaattt agggatataa atttccgata caagggctta tatacacccg
    28381 acaagaactg aacgcattga acggcaccac gctctgccag tttgttaagg tctgggatag
    28441 ggttcctgat gccagtgttg cgaataattt cttgaattcc tttgggtttc tcaggggaaa
    28501 ggtagttcaa aacaattctt tgtttttcgg gtagcgaatg gtagtcgctg aatgtgtcat
    28561 tttttatgta acggctttca ctgtcaggtc gtatacccgc gggcagcgct gctttcatta
    28621 cctcccctat tgaacagcaa tagtattgtg caacccaatt ccatagttcc atctgtgcca
    28681 tgttggttac aggttttgag gagcatgaga gtatttcttt cagttttaac tggctatcgg
    28741 ggtgttcggt aaaccctgaa attataccgg tatagagttt ggatttacca aaattaacca
    28801 ccaccgaaga cccctgttca gcaatggagc ataggctatc gggaactata taggtaaaac
    28861 tcccgttgag tgctacaggt ataattattt gggcatattt cataaattta gcgactgacc
    28921 gtatgccttc ttatcaagtg tttaaaatat aaagcatttc cagcattttc cggtttatgt
    28981 ctttatgatg ttttattgcc tgattaaagg gtataagagc ggtttcattt tttatcagac
    29041 caaccatcac actgcgctga tcgtcgagta atgcttctac ggctgcaagc ccaagtttgc
    29101 tggcaagata cctgtcgaaa gccgaaggag cgcctcctct ttgaaggtga ccaagtatag
    29161 aaactctgat atccatatca ggcaaacgct gtttaacaat ttctgcaatt ttcatcgcac
    29221 ctccggcatc atctccctcc gaaacgataa ccactccgct gtttttagtt ttttgcacgc
    29281 tgttggcgag gttggcacaa acttcatcca tgttggtctc aacctcagga acaagaacat
    29341 cttcagcccc gcaaccaatg gcactgcgca gggcaatgaa gccggcatcg cggcccatta
    29401 cttccacaaa aaataaacgg tcgtgggaac ttgctgtatc tcttatttta tcaatagcct
    29461 gaatggctgt attaatggct gtatcataac caattgtcat atcagtacca tacagatcat
    29521 tatcaatggt ccccggaagt ccgattatcg gataattgta ttcttctgta aataatttgg
    29581 ctcctttgaa agtaccatct ccaccaatag caatcaaggc ctcaatacca gcttccacga
    29641 ggtttctgta tgcgatatag cgaccttctt tagtcctgaa ttcatcactg cgggcggtcc
    29701 ggagaatagt tccaccccgc tgtattatat tgcccacatc gcgtgatgtc atttccatga
    29761 agtcacccag aatcattcct gcatacccgc gccttatgcc ggttacgctc ataccataat
    29821 aaattgccga acgaacgact gctctgatgg ctgcgttcat accaggagca tcgcctcccg
    29881 aagtgagaac cccgatacgc ttaaattttg tcatagagat tcaaattaat ttatgctaag
    29941 ttaatttgta tattgttata ttatcgtggt gttgaaaaat aatttttaaa acctaaagaa
    30001 aaattaactt agtttctatc tgtgtaaata aaaaaagtcg aacgaaaaat gaattaaacc
    30061 agaattaaaa catcagtttt atttccacca aagttaaaat atcattaata atcgcggaat
    30121 catgaacagg gatgcgaata cctctgcctt cggcatacac ttttgcttcc gacaactctg
    30181 ttttaattgt cgcggaaacg tctgtaccca taatggcttc aaatgctttg gccccgatgt
    30241 taaaggccgc catgaaaaag gaatcacggg gtaacaagta aacaattact ctctttttat
    30301 cgaggatacg atagctccac ccgtactttg ccccggggaa agcccattcg gtttgcaaac
    30361 ccgtttgctt ggtcataata taatcttcta tgtttctcca caacgggtaa gtcgctttaa
    30421 gagcattcct aagcatggta tcgctgggaa tgattatttt atcggtaaaa acgctgcaat
    30481 ccatagcatt cggatttctg ttagcaataa ttaattttag tcagacagca caaaattacg
    30541 aaaacaaaag cagaattttt ttaaaccttt ttcattgcta tttttgtgca attaaaacaa
    30601 acacaatgaa tggagaagaa ttttttaaaa ccttgatttc gcactgcaag gagtatggtt
    30661 ttatatttca atcaagcgaa atttacgacg gactgagtgc tgtgtatgac tatggacaga
    30721 atggagcaca gctgaaaaac aatatacgtg actactggtg gcgggcaatg acacagatga
    30781 acgaagaggt ggtaggaata gatgcagcca tttttatgca tcctacggta tggaaagctt
    30841 cggggcatgt cgacgcgttc aacgatccgc tcatcgacaa caaagattct aaaaaacgct
    30901 accgtgccga cgtactcatc gaagattatc ttgcaaaact cgaagataaa gtcaataagg
    30961 aagtagagaa ggcattgaaa agatttggcg aaagcttcaa cgaaaaactt ttccgtgaaa
    31021 caaatccgcg aattcttgag tatcagcaaa aatctgatga agtgagggct gccatgacaa
    31081 cctgtcttga agagaatgac ctcgcaggaa taaaaaggct aatcgaagaa ttaggaattg
    31141 tatgccccat ttcgggttca agaaactgga cagatgtaag gcagtttaac ctcatgttcg
    31201 atactaaaat gggatcagtt gccgaaggtg ccgatacaat ttatcttcgt cccgaaacag
    31261 ctcagggtat ttttgttaac tttctgaacg tgatgaaaac cgggagaatg aagcttcctt
    31321 ttggaattgc acaaacaggt aaggcattcc gcaacgaaat tgttgcacga caatttatat
    31381 tccgcatgcg tgaattcgag cagatggaaa tgcagtattt tgttaaaccc ggctctgaac
    31441 tcgagtggtt tgaaaaatgg cgcgaaaaac gtatgaaatg gcatcattca tttgatttcg
    31501 atactgcaaa atatcgctgg cacaagcacg ataaactggc gcattacgcc aatgcagcct
    31561 atgatattga atatgccttt ccgtttggct ttaaagaaat tgaaggtatt cattcccgta
    31621 ccgatttcga tttgatgagg catcaggaat tttcggggaa gaagatgaac tatttcgatt
    31681 cggaaacagg tgaaagcttt gttccttatg tggttgaaac gtctattgga ctcgacagaa
    31741 cattccttat ggtgctgagt gcagcttata ccgaagagca ggtaggaaac gaaatgcgta
    31801 ttgttcttaa aattcctgcc ccgttggcac cggttaaggc agccgttatg cctctggtaa
    31861 aaaaggacgg acttcccgaa attgcacgcg gtataattga tggacttaaa tttgatgtgg
    31921 tttgccagta cgatgaaaaa gattcgattg gtcgccgtta ccgtcgtcag gatgccgttg
    31981 gtacaccgtt ctgtattacc gtcgatcatc agacaactac tgacaatact gtaactatcc
    32041 gctaccgcga cagtatggaa caggaaagaa tacccattgc gaaaatcggt gacattgtac
    32101 gagaaaaagc tgatatccgc aatttgctca agcagttata ggatgataga gatgtctaaa
    32161 taattaaaag tgggaaaaaa taagtttttc ttattgtttt accttactgt tgattttatt
    32221 taaactagag aacagtgccg gttttttata aaatcaatca ttaaaataat cgtcgagact
    32281 ccaactgcct acactgacca attcatcggt attttgaacg gaggcgttgt attgccggag
    32341 tttattagtg atttcatcgg ctttgtcatc gcagagtggt acaaaaataa tattgttata
    32401 tccgggccac accgaagtat tcatacgatg ttttccggtg aaaacccggg cagcaacatc
    32461 gtcggaaatc tgatactcat caatacctgc tgcgtcgagc agttctgtta ttatatgggt
    32521 agtgcttacg ttgcacgata tgtaaatcat tttcatatta tctgtttttt tcgtttttca
    32581 gtgctttctg attcagcgat aaatatatgc agggtataag gtacaaggta atcagcaacg
    32641 aaacagtcaa ccctccgatc atggtaatag caagcggaat ccacaattcc gaccccatgc
    32701 ttttactgaa aagcatcgga atcattgcca gaatggtggt gagcgatgtc atcagcaccg
    32761 gacgcaatct cgacttgccc gcatcaagag cagctgttgt gatcgaccat ccacgtgccc
    32821 taagtaggtt ggtataatca acaagcacaa tcccattgtt cactactatc ccgagaagca
    32881 tgattactcc cacgaaactt acaatattca gttgtatgcc ggtaattaca aatgccagaa
    32941 ccacaccgat gatagcgaat ggaatggcaa ataaaatgat gaaaggatcc ttaagcgatt
    33001 caaactgcga tgccattacc ataaaaacca ataccagtcc gataataaaa atccactgga
    33061 ggttggaata cgactcttct ttatcgagaa cttcaccact gatggcaata tttacagcat
    33121 ggtcggcatc agtttttgat atcatctccc ttacaatttg agcagcctcg ctcagggtga
    33181 taccgttcaa ttcggctccc acggtaacaa aacgcgattg tgtttcgtgt ttaatttcaa
    33241 gcggaccata gctctctttg atttcggcaa cattcgataa agcgacctgc gaacccatta
    33301 gggttgaaag gtttattttt ttcaggtctt caatgctgtt acggtaatct tttccgaaac
    33361 gtaccactat tttataatcg tcgccttctt ctttataagt tccggcttct gccccgtaaa
    33421 tcccattacg tatctgcaag gcaatcattg cagtgttgag ccccatcaat gaggctttat
    33481 ctttatcgat atcaactaca tattctagtt ttccaagatc aattgaagat tccacgttac
    33541 tgaacctctg gtcgcttttc agcaattcca cgaagtataa tgctgtttca ttgatttttt
    33601 caaaactttt tccggagatt tttatttcaa cgggtttgct gttgcctagt agcatgtttt
    33661 gcaacattga acctcctatt acctgataat tctcgatttc aggtatttcg gcaagccgca
    33721 aacggataac ttcagcaatt tcgattgctg tacgttgcct gtattggggt aaacaaaggt
    33781 gagcgcttat tgtggctctg tttttccctt ccttgaaccc gatgccagta agcagattcg
    33841 attccgattg acctacaatg gtatattgcg ataccatttc gggtatttcg tcgttaaata
    33901 ttttttcaac ttttttggct acgcgctccg tttcatcaac attcgtacca acctcaagct
    33961 caatggtggc aattatatcg cctgcatcca aatcggggat atagttgttt cccagacggg
    34021 tgaatatgaa tattgtgagt agaaacagca atagggcaaa gcttataaat aagtatttaa
    34081 accgtaccac atatttcagg ctgcttcggt aaccgttttc aattgaattg aagatgttct
    34141 cactactcct gaacagacgg cttctctttt gtcggtgctg tgcctggttt ttcaataata
    34201 acgaagatag catcggggta agggtgagcg cggttaacaa agaagcagcc attgttaccg
    34261 aaaccagtat tgccaactgt ttaaaaagta ctccgacgag accaccggtg aacatgagcg
    34321 gtataaaaac acatattgtt gtaagtgttg atgcggttat ggcgttcccc atttctccgg
    34381 tggcaaaaat ggcggcctgt ttggggcgcg ctccgttttc gatgtgcttg tttatgtttt
    34441 caagtacaac aatggagttg tccacaacca ttccaatagc tacgatcaac gatataagcg
    34501 aaaatatgtt cattgtgtaa ccaagcaact tcatggtgat aaatgcaaac accatcgaaa
    34561 ccggaatcga tagaataata atcaggctgt tgcgccattc cctgagaaaa attaatacta
    34621 caattaaaac aaatagccct gcataccata tagtggagct taaattagag gtgacttcct
    34681 ttatgatttc agcagtattg aacacaatat ttgttttaac atcagccgga agattcttct
    34741 gaattccagc catctcttct acaatggcgg tataaacctc atatgtgttg gcatcggtct
    34801 gcttctgaat aaataaccca acggccgttt ttccatcgct atatgctttt tcgtccttct
    34861 cacgataccc atcagttatc tcagcaatat ctttcatccg gataattcta ttgttgaacg
    34921 aggtgatggc aatttcccct atgtcttgcg tgttcttaac ttcgcctggg atacgaaccg
    34981 caaaatcgta cttgtcgaac ctgacatttc cgccgggtat cgtaatgttc tcggcctgca
    35041 ggatggttgc aatctgtgta atgttgaggt tatatgcttt cagttttaca gggtcaacat
    35101 tgatacttat ttcgcgggac ggctgaccga ttatgaaagc agatcctaca ccgggtactt
    35161 tttttagggg gtcgatgatt tgctcatacg cgatcttttc gagtccgttg tagcttatgt
    35221 cagcctgaat agtataaacc acaacaggaa tcattgagct gttaatttta agtatatacg
    35281 ggctcatcgc atctaccggg agtttgcttt ttgcaagctc catcaggtcg cgggcagagt
    35341 ttactgcatc gcttacatcg gtgccccaat cgaattgcat cgaaatgaac gaaacatttt
    35401 cgcgcgatga tgaggataat tttttcaggt tttctgttcc gcttaacact acttcaaggg
    35461 gattggtaac ctgcgactca acatcttctg cagaagctcc tggataaata gtaataacag
    35521 taattgcagg aagctcgatg tcaggcatca ggtcacgtgg aagagaaatc catgaaatga
    35581 caccaaaaat aaccagcgca ataaaaacca ttgcagtggt taccgggttc tgaacggtta
    35641 gtttgggcag tcccatgatt agttgttttt aataatcacc tgttgattgt ctttcagctt
    35701 attcttgccc tgagtgacaa ttgtatctcc tttggatatg cccgaaactg ctacccactg
    35761 atcgataatt gcaattcttt tcaccgccat ttggtgtgca acgcccgaat gtaccacgaa
    35821 aataaaatca tcaggagtgc cgggctgcct ataaatggca tcaactggaa caaataccgc
    35881 agaactccct tcggtgtgca acagtacgcg tgcaaacatt ccgggtttaa gccggttccc
    35941 cggattaggc acctcaatct caacttctgc agtacgggta actgtagaga gcattggttt
    36001 aatgtatttc acaaatcctg tgagtccgtt acccgaaact gcatcgcaca tgatgctgac
    36061 tttctgtcca ctcttcaact gttgcaactc tttctcattg acctgtattt ttacaaccaa
    36121 cgggttgatc tgcatcagac gtaaaatccc tgacgtattt gagtaaccgg gatcaaaatt
    36181 caggttgaag aaatagtttt cgccttcttc tacaagatat tcaacaataa taccatcgaa
    36241 aggagcgact acggatgtgt ttttgtgtag catttcggtt ttcacttccg atgcttccag
    36301 cttggctttc aggtgatcgt attcaatttc actgatactt cctttcgctc tgagacgttc
    36361 aacccgttca aagtcttttt ttattgcatc atattcaatg agtgcctggg taagcaattc
    36421 agccgacatt tccacaagca gtgtaccgga agtaacaaaa gtgccttcag ggtaaaaaaa
    36481 cttctcgatt ttcccaggca ttgaagcacc aagattggct tcacggttcg gaaagaacgt
    36541 gccggagtac tccagctccg gcgaaaactg tttttcaacg gcaatagtag ttttaacaac
    36601 cggattgttt tcaacttttt gtggttgttt gtcatctctt ttacaagaga taattagcaa
    36661 aattaatgca aacagatata tcgcgaattt tttcatatac tgagtgtttt ataattgccc
    36721 ggtttttttt cggtaattga tctcctgtaa ttttaggtct gtttttgcct gaataagctc
    36781 agagtaagct tgttgccata aaacctgagc ttcgagcagg tcggctaggg taagcattcc
    36841 ttcctgatag gcatcattag ccatgcgaag gttttctttg gactggatca gtgctttttc
    36901 gcacaggtcg tattttttca tggtttcggt gtacacaaaa tatgcttgct ccaattctag
    36961 ctgtatcatc tgttcagcat cttgcttcat tagttgcgat atctctatta ttttttttga
    37021 tgcgcgcata gtatgcagcc tgtcgcccca gtgaaataga ggtatctgca cggtaactcc
    37081 caacgaccag tcgctgccaa attctttttc gaaagcgttg tagggattgg ggttgacgaa
    37141 aaggtaattt gctgaagcca taatattggg tagaaaccgc gaaagtgcga tgtttttctg
    37201 agcaattgcc atattcattg tattgtcgag aatatgtagc tcttctctgt tctgcaaagc
    37261 cgtttcatag ctcccagaag gattgggtag cacaggaagc ttgtcgagat taccgtcaag
    37321 cgaaatctgt gtattcaacg gtaaacctat tatctgtagc aatgccatct gcgacaatgc
    37381 caggccgttg tcggctttca gacagcttaa ctctgcttcg ttcaatttta attttgcttt
    37441 taatacatca ttgtaggtaa ttatcccttc agcataaagg ttttcgaggt ctccaacaag
    37501 atgattaagc agttcacggt atttgtatgc caatgccttt tgctcctgta cacttacaca
    37561 ccgccagtat gcttcttctg taagaacaat tacatcatcc tcatcgagaa gttgcttcga
    37621 tttcataatt tcttctcccg cttcggcaac ttttaccatg tttcttattt taccccccag
    37681 ataaatgggt tgaagcaatg acggcccgaa catgaaattt tgatgtgttc cgaatttcag
    37741 ttgatcagag ggcaaatagc catacaacca gaaggcggga ttaccctggc tgtcgtaaaa
    37801 tatttcacct gtaagagggt tgcttacaat tccgttcaga agtgggttct cgagaatgtc
    37861 ctcgcgcagt gttcccgtgt tttcatcaat tgcccaaaag ggaaccacag gcaaaaaaag
    37921 atcctgattg aacaagctta tctgacggtt actgtactga tattgcccca taaaaccaaa
    37981 ctcaccgaag tattttgtga agcttgcttt tcgcatttcc gtggcagctt gtgtgcggca
    38041 ttctgctatt ttaattttac tattgtgctt gaacgccata tcgcggcatt gcccgagatt
    38101 taaatataag gtgtcggttt gagaatgcag cgggtttgca ataaaccata ttagcaactg
    38161 caccagagca atacgtttaa aataagtcat acattcatct ttttaccaaa ccgaaaaaaa
    38221 cgatgtcaat aattgtattc aactgctttt cgaatacttc ctgttcatag gaaatgagta
    38281 atggaagctc taccgcttta atagttaaat gaaatgtttt ggcaacatgc tctgggtttt
    38341 cgattgtgaa aactccgttt tcacaacctt gtttgagcac aagggtaaaa aattcaagct
    38401 caagtttatc gtatttcaac cgcgcctttt gtacaaattc gaggtgattc agaaaatcgt
    38461 tgcgcaatgc ctgatataaa ttgcccagtt taataatctc gttaaaccgc accactatat
    38521 aatttctcat tttatcgagc aaattttcgg gctcgcggaa cgccgcggct aatttttcac
    38581 gcaacatatc ggcctcatac tccaccactg cctggaacac ttcttcctta ctggtgaaat
    38641 agtagtagag tgagcttttg cctttacggg cttcaatggc tatttcgttc atggtggttt
    38701 tttcgaaccc gaatcgtgca aacatttgtc ttgctgattt tattagattc tctctgactt
    38761 tatcgcttct attcatactt ttcgactcaa atggtattaa aagtcaaaat taatacaaat
    38821 attcgaattt tcgactcaaa aaatctaaaa agtcgaaatt tcagaaaatt ttattttggg
    38881 ctaatttttc cttaacatgg agttaatgca aaatcaatct gccattgtca aatgctgatt
    38941 tttgttatcc ggtatcaact aaaaaaaatt tagtgatcaa gcacttcgta aggtgaattc
    39001 tggctgataa attctggtac aatttctttc atttttttta caagtgccat attttcgcta
    39061 ttgtcaatca tttgtactaa tgtttcgatg tggtcttgta tggtattgaa atcgtattcc
    39121 cttacttttg caatcattat tcgggggtgg taggtgggta cggtattttc tttggttgca
    39181 agcagttctt cgtacagttt ttcacccggg cggaggccgg taaagcgtat ctgaatatct
    39241 tcgcccagtt taagacccga aagctgaatc atccttttgg caagatttac aatttttata
    39301 gattcgccca tgtcaaaaat aaagatttcg ccgccttcac ccatactgcc ggcttcaaga
    39361 accagttgac atgcttcggg tatggtcatg aagaatcgtg tgatttcggg atgagtaacc
    39421 gtaataggtc ctcccgactc aatctgtctt ttaaatcgtg gtattaccga accatttgag
    39481 ccaagcacgt tcccaaatct tgtagtaatg aatttgcatt ttttaaactg attcagcgat
    39541 tgaatataaa tttcggcaat acgttttgag gcgcccatca cgttggtagg gttcaccgct
    39601 ttgtcggtgg ataccattac aaatttaagg actttgaatt tcagcgatgc atcggccaga
    39661 agccgtgtcc ctgtaacgtt gtttaaaatt gcagcgcccg gattcttttc catcatcgga
    39721 acatgcttat aggcggctgc atgatatacg aactcagggt tgaagctggt aaatactctt
    39781 tcaaccaact cggcgttggt tatatcgcca attacaattg taaaattatg aaagccaagt
    39841 ttttcttcaa gctccagttc aatgtcgtaa agctgcgatt cggcttgatc aaaaaggacg
    39901 agatgttttg gattgtaatt ggtaagctgt cgcactattt cgcttccaat cgacccggct
    39961 gcaccggtaa tcagtatggt tttgtcgaga agttgcttac gaatatgctt tttatccatt
    40021 ttgatttccg gacgttgcag caaatcttcg attttaatat tctgaatctg gtgaaggctc
    40081 aattccccgt taatccattt agaaacatcc ggtattactt tgatttcaac atcgttgtcg
    40141 aggcagatat cggttatttt ttgttttatt tctaccggaa tttgcttttt tgcaataatc
    40201 agggtactga taaactgctt ctttatttgg gtttccagat cgcttatatc aaaaacccta
    40261 atcccttcga ttttatttcc cgtcatttta gaaccgaaat cgaaaaaagc aactacttta
    40321 tcgcgtgatt cggtatctcg gtcaatggtg cgctgtgtta ttagggcaag ttcttcggtt
    40381 ccaaaaacag ccacagaaaa tgcctttcgc ctgggattgt tgaactcaag aaaaacagct
    40441 ttgactaaaa ggcgtgaaaa gcttaggata aaagcggttg ttataaaatc gataataatt
    40501 accgacatgg gtatgagaaa cctcttttgc agataataag tgatgacatt taatattacc
    40561 agaattgcac tgctgatagc tgaaacaaga ataatgcgtt gtatgtcgcg ggtactggtg
    40621 tgccgaacta taccagcata tgagcgggaa acaataaagc tgattgcccg gatgctgaga
    40681 accattggta aaacatacac aaaacgggca atttcgtaag caggtacttt aaagttgaac
    40741 ctaaggtaat aggcaaaaac aatcgaaaat aagcatatca tcaggtcgat taaaaaaata
    40801 acccaccttg gtgttatact tctcagaaaa ttaatattca tagctgaatt ttaatttgtt
    40861 ctatccttac ctggttcatg cttttgatgc tctgaaatta ttatcagcaa atgtagaaaa
    40921 aaatcaatga ttacactttg ccttttgaat gattcatttt tattgagcta tctaaattat
    40981 tttttaattt tatgaaattt tcgtcgcatg atcaggctta aaatcatagg tttgcttctt
    41041 ctttttggtc tcctttcttc ctgtggtatc agagtgaagt atgacgaagc ggataagaaa
    41101 tgccggaatg cacccgatgt ggtacttaaa aagtttgatg ggggtgtttt ctccatcgaa
    41161 ataccggaat cgtggaaagt aataactgcc ggaaattgtg aaactttttc gttttgtgct
    41221 tacgatagta tcaagcctgc caatcgtgtt tttttgctgg gcgaaatagg cccggtgtat
    41281 cagcatatcg aaaaaaagcg tgaggatttt cagtcgatac aggatatggg gttagatata
    41341 ggttatttcg atatgcctgt tgtgctgccc tttacaccgg agtgttattt tgagaatctt
    41401 tacctaatgt tcgaaacaga atgggcaaaa gcaatgaaac ctatttgccc tgattttcat
    41461 aatacaatta ttattaattc cgaaacaata acacctgtta tgtccgatgg tatttccaag
    41521 gtgttgagag caatacattc atcgaacgac acgatatacc agacacttat tttactgaat
    41581 ttggcaccgt tctacgaaga gatgggaaaa acgaagaaag gacttggcaa ggcttatatt
    41641 gttcttggcg tgaatgctga attcaacgat tttgaggcat tactgccgtt aatgttacaa
    41701 attgccgcta gttttcacat gaacgaaaac tatattcaaa attgcagtgt tggtgcgtcg
    41761 gcatttgcaa tggaacagat gaaattgtcc aaatcgctta ctccttatgt gacaataatg
    41821 cgtgacgact ggaatgcaag ggataagtcg atgaagtaca ttattttgaa aagttgtgat
    41881 tcccttttga ataaaatcag aattttcgat ccaaataccg gaacacctta ccttgtttca
    41941 gcttcttttt tcgataaata catcaactca aaagaggagt ttgaaatgaa caaactgaaa
    42001 attctgtcgg ttagcgatac cgctctttgg ttaaaaacac cgcttgactc aagctatatc
    42061 cgataatcac ttattatcag gcttttatta tgttggcctc ttcaattaaa ggcatcaacc
    42121 tgtaagaaag tagtagctgt gcaaccgtga gaacatcttt ttcgcaatag gtaactatgc
    42181 ggttcagatt cttttcgttg tagtaaacat ctgccaccat gcttccatca atatcatctt
    42241 tcggggtggg tatgccaaac aggttagcaa gtaattcgag cgaagtataa ttcttaaaat
    42301 ctccgaattt ccatagttcc atagtgtcga gaagcttcat gtcccatggc tttttaccgg
    42361 cattgttcag catttcaggc aatggtattc gattcacaag cattcttctc gaaatatacg
    42421 gtatatcaaa ttcacggata ttatgtccgc agaaatattt atcacctttg ggagcaaagc
    42481 tatcgagcat ggcagaaaac tcggtaagca gttctttttc ggtatcacca gaaaacgact
    42541 ttaccctaaa atgcttgtcg ccgttgcgtt ctgttataaa gccagcagag atgcaaatta
    42601 tttttccgaa ttcagcataa atcccggctc tttgatagac atcagaagcc gattcgtcag
    42661 catttcgaaa aaacgatgat ttacgctccc agtgcttcct gaaactttcg ctaagttctt
    42721 cgtaaaccgg aactgccgga accgtttcga tatcaataaa aagcacctga ttcagggcaa
    42781 tcttgttgta atcaattatc ataacttagt tttttaattt tatttctata aagtgagtga
    42841 gcaattcaat ggctacgcta ttgtttcctc cttgcggcac aataaggtca gcatagcgct
    42901 ttgtgggctc aataaattga aggtgagaag gtttaaccgt tttttcatac ctttccagta
    42961 ctttgtttac cgacctgcca cgttcaataa tatcacgctg gattaccctg ctcaaccggt
    43021 cgtcggcatc ggcgtccaca aatactctca ggttcatcag atcgcgaagt tctttgttcc
    43081 cgagtataag gatgccttca ataattacca ctttatgcgg acttagcggt ataacatctt
    43141 ccagcctggt gcatgtaaga taagagtaca taggttgcat aaccgtttca ccacgtttca
    43201 gtcgttttat gtggtcgatg agtaagctcc attcgatagc atcggggtgg tcgaaattca
    43261 tttcctgtct tttctccaac ggaagatcag aattatcgcg gtaatatgaa tcttgtggaa
    43321 ttacagaaac ttcatcttct gaaaaacgct ccattatttt ttttactact gttgttttcc
    43381 cggagcctgt gccaccggca attccaatta taagcatatc aaaagaaatt tatcgtattt
    43441 ttgaacaata aaaataaaga atttacttga gattttatgt tatacccttt aaaattcaga
    43501 cctatttata aacagcgcat ctggggtgga aatcgcctcg cttctttgtt taataaaaaa
    43561 agtgccccgg ctaattgtgg tgaatcctgg gaaatcagcg gattaccaga gaatatttcg
    43621 gtggttacta atggatatct taaagggaat aatcttcagg aactcatcga agtgtatatg
    43681 ggcgatctgg tgggtgaaaa ggttttcgat actttcggac tcgatttccc attgttgatt
    43741 aaactgattg atgctaccga agatttatcg tttcaggttc accctggtga tgattatgct
    43801 gccgagaatc tggaaagcta tggcaaatcg gaattatggt atgttttaca tgcagcagag
    43861 ggagccaaac tggtatcagg ctttaaccgg accatcactc gcgaaacgct gcttgaaaaa
    43921 ataggcaacc attccttgcc tgaagttgcc tgctacgaaa atgcccagta tggtgatgtt
    43981 ttctatattc ccgcaggcag aatgcatagc cttggaaaaa acattgttct tgccgaaata
    44041 cagcaggcaa gcgatattac ttatcgtatc tgggactttg accgattcga cgcaaatggt
    44101 aaaaagcgtg aactgcatat tgaacaggct cttgacgtcc ttgatttttc aaaaacagtt
    44161 gatcccagaa cattgtattc aaagttgccc gataaaggtt catcaataat cacaacaccg
    44221 catttttcgt gcaacattgt atgccttttc aataacctgg aacgtagcta tgccgataaa
    44281 gacagctttg ttgttattat ttgtgttctt ggagaggtga acctgcacta taacaacgaa
    44341 aaatataacc tgaagaaagg agaaacaatt ctcattcctg ctgaaattag cgatattacc
    44401 tatgaaccgg atggatatac tgagtttctt gagatatttg tactaatgga ataattcgtg
    44461 gcagttgctc ttttgatgat actaatttta taagcatacc gttattaatt agttatattt
    44521 gaaagtagta ttattaacta agattccata acaatgagaa ctttagtcct gttaattgtg
    44581 tttttcagtg ttgccatcgt tttttcggca tgtaaaaaac aaaacaacga aattgttgac
    44641 ctgaagctta cgacacaaca aaaaaacatg gtggtttcgg gcaatcattt tgcatttaat
    44701 atgctgaggg aagtatcaga atcggaaact gcacccaaaa acatcatgat atcgccgctg
    44761 agcattaatt ttgcacttgc aatgactgcc aacggcgcaa aaaacaatac gctcagccag
    44821 atgctgacca ctatggattt tgaaggttac gaaatggagg atttcaacga atatttcaga
    44881 tacctgaagt cggaacttgt tgccctagat ccgcgcgttg agctttctat tgcaaattct
    44941 atatggtatc gtaataattt taccgtgctg caatcatttc ttgatgtaaa ccagaattat
    45001 tacgatgccg aggttgcctc tctcgatttc agttcaggtg atgcgctaaa tacaatcaat
    45061 gattgggtca acgatgaaac ccatgggaaa atcgaaagga ttctcgaaga aataggtccc
    45121 gatatggtaa tgtttcttat caacgcagtt tatttttacg gggagtggaa atatgagttt
    45181 gaagaatcgg ctacatacga tcagccattt tatctgcagg gtgggggaca gattgatgtt
    45241 tccatgatgc atcaggaagc ggaaatgaag tattattttt ctgatgaggt tgcgatcgcg
    45301 gagctacctt acggacaggg gaatttcgtt atggatgtaa tattgccacc aatagaacag
    45361 acggcagcag aattggtatc aacgcttgat tcagaaaaat ggactcaatg gcagaacgga
    45421 ctgtatactg caaaggtttc gcttggtatg ccgcggtttg aattcagcta cgaaaaaacg
    45481 ctgaatgata tgctaattgc actgggtatg aacgatcttt tcgacagtgg gttagctgat
    45541 ttatcaggaa ttaatggtac cggaggactg tttgtaagcg aagtaaagca caaaaccttt
    45601 atcagtgtcg acgaaaaagg cacagaagcg gcagcagtta caagtgtggg agttgagctt
    45661 acttcagttg atccaggcgt ggtttatcta actcttgacc gaccatttgt gtttatcatc
    45721 agggaggtta caactggggc aattctcttt tcaggaattg tttcgaatcc ggtagcggaa
    45781 tagattttta ttgaatattt tcagccaaat tggtaataca gctggccact tcttccatca
    45841 accataaagg tgtgctggtg gctccgcata ttcctacatg gtcatctgcc ttgaaccatt
    45901 cgggttttat atcttcggga cctgatacaa aataactcct ggggtttgtt tccgagcaaa
    45961 gattatacag catttttcca tttgagcttt tggggtcact tacaaacaac acaatgctat
    46021 gttcagttgc aaatttttgt ataagaggct cccgttttgc cacaagcggg catatactgt
    46081 tcagaaactc gatattacgg tgatgcccca gaattcgagt cgccctgttt tttgcctcaa
    46141 caataatatc tcgatacttc tcggggcttt gtgtggtttg tgagaaaaaa aataccggct
    46201 tgctgaaatc aataagatta aggtcttgca tttcgcttac aacaatagct ttactatggg
    46261 tttgtccgac caatccattc acttcggcat gttgtttttt accatagatt acaatttgaa
    46321 tacctttttc accgtttgcc tctgcttgtc tgatttttcg ctgaaggttc agtaccactt
    46381 tgcaggaagc atcgataagt aatatgttat ttttttttgc agtattatat gtttcgggag
    46441 gttcgccatg agcccggatt aaaacggttg catctttcag gttctggaat cggttgtaat
    46501 caataataat taatcctttc tttttcaggc gctctacctc aatagtattg tgcacaatgt
    46561 cacccagaca atacagcact ccgttctgct caagttcgag ctctgccatt ttgatggcat
    46621 tggttacacc cgggcagaaa cctgaatgcc ggtcaatttc aattgtcatt gatatcgagt
    46681 tgtttattca taacaacatc aaccatggca agtgctacca tcagagtttc ttctgcattg
    46741 atatcgctgt tgtcgagcaa tatggcatca tcagccattc gaagcggact ttccgttcgc
    46801 gactggtcca gataatcacg gctaattaca ttctccagca cttcagtaaa acttacctga
    46861 atcccattat cggtaagctc cttgaagcgc cgttttgctc ttacttctgc tgaggctgtg
    46921 agaaaaattt tcaattcggc gtttggaaat acaaccgtac caatgtcgcg gccatccatt
    46981 accacaccgc cgttctccga aagcttacgc tgcaatttaa cgaggtgttt tctgatttcg
    47041 gctacagcgc ttaccttgct aacagccgcc gaaacctcaa gactgcgtat cttttcttca
    47101 acatcgatat tgttcaaaat agtccttggc ttattgcaac ctaatttgag aaaacggatg
    47161 tgtattttgg gcaatgcttt aatgagcttg ttttcgtcaa atccggaagc attgattaat
    47221 tcgttttcaa tagcatacag agttacagcc cgatacatcg caccgctgtc gatataggtg
    47281 tagttgagtt tttcggctag tttacgcgcc aatgtgcttt ttccgcatga agaatgtccg
    47341 tcaatggcaa taatgatatt ttttttcttg atcaaaacag tggaaagcaa tatttaggaa
    47401 ccattcttct tataaaaaga gttgagatta gttgatagcg aaatcatatg cgatttaccc
    47461 gaaaaatgat aattggcaaa gctataaccc agattaaatt tcgatatttt cacacctgca
    47521 cccaccgaga atcctagca
//
LOCUS       DAOOQP010000008        47396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_836_length_47396_cov_5.317336, whole genome shotgun sequence.
ACCESSION   DAOOQP010000008 DAOOQP010000000
VERSION     DAOOQP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47396
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_47396_cov_5.317336"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="PLK75_01640"
     CDS             <1..1297
                     /locus_tag="PLK75_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37978.1"
                     /translation="NYGQWPVNDGSGDILVDDLIFDYTPVQGNSYRVTGVLDFSFAEW
                     KIQPRSAEDIVDLGVNTQPTLYINSPENGSTIYTASTNFGFTVSNFTLGTDGKVEYIL
                     DSQTPVYVTTSPSAITGLTNGEHTIVAQLVDMSENPLNPAVTATITFTVNLSGPTLTS
                     IYDIQYTDQASGASPLENQTVSIKGVVSAVFNGTPYGEGYYLQQGSGAWNGLFIYDLN
                     NTPALGDSVLVTGKVSEYYEMTELTNITLFQVTGIGGTIANPTVVSTLDANTEMYESV
                     LLKVMNAECTAVQNTYGQWTVDDGTGALLCQDNGAFSFEETLGTHYNIIGVTIYSYEE
                     TSLNYRIPSDIVESTSIETNLSNSTLIFPNPANNLITISANEKLTNLNIVSAEGKTVM
                     TISPDSRDVTIDISQLSSGFYFIHLEGQYQKGVKKLIVE"
     gene            1398..1841
                     /locus_tag="PLK75_01645"
     CDS             1398..1841
                     /locus_tag="PLK75_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOY37979.1"
                     /translation="MKKKYMLIFFSVVLVFSTSCTKFEEGAFISLRSAEFNIMGTWKI
                     SEVLENDLVNDSLLNEEANNLLVFADSSSFTFQVLRNGNIAQNLAGTWQYNSDNQHLS
                     FKTDADSTFQVYRIIRLTNKEMWLVDECQDQTRSGSSIEKRYQKQ"
     gene            1858..2304
                     /locus_tag="PLK75_01650"
     CDS             1858..2304
                     /locus_tag="PLK75_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37980.1"
                     /translation="MRKIASILLIAAAAVFVFNSCQPYEEGPSISLLSKTTRLVGDWT
                     IDTWLANDTEQDISSTQPRMSFVKDGSGSMTINVFNFAVTVDLEWEFVGDDGIRYRVK
                     NQNDAWEDWTEYTILRLSNDELFIERTYLPEGSEANVTERIELSKN"
     gene            2388..4415
                     /locus_tag="PLK75_01655"
     CDS             2388..4415
                     /locus_tag="PLK75_01655"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOY37981.1"
                     /translation="MTKDDFKKQILEGIPKTLPSMKHPNPQLNHAPKRKEILSEIEKK
                     LAIRNALRYFRKEWHPVLAQEFKEELEKYGRIYMYRFLPDYEIFARDISWYPHKSEQA
                     AAIMLMISNNLDNAVAQHPQELITYGGNGAVFQNWAQYLLTMQYLACMTDEQTLVMYS
                     GHPMGLFPSHREAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGSYMYIGPQ
                     GIVHGTTITVMNAVRKINKTNTSSEGVLFVSSGLGGMSGAQPKAGNIAGVVSIVAEIN
                     PKAAGKRHAQGWVDEITSDIDHAIDRALEYKKTNRVRSIAYLGNIVDLWERFAERNIP
                     VDLGSDQTSLHNPWAGGYYPAGLSFEESNIMMSENPEKFKEHVQKSLVRQIRAINKLS
                     EKGMYFFDYGNAFLLESSRAGAEVMAADGISFRYPSYVQDIMGPMCFDYGFGPFRWVC
                     CSSRPEDLDLTDSLAKEVLLGLSQKAPIEISQQMHDNIKWITEAKKNDLVVGSQARIL
                     YADAIGRIEIAKAFNKAIRNGTISAPVVLGRDHHDVSGTDSPFRETSNIYDGSRFTAD
                     MAIQNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMVLDGSAEADRRLEMMLYWDVN
                     NGISRRCWARNEGAIFAAERAMKTNKNLSITLPYIADDSIIDSLFNQNNRK"
     gene            4412..6469
                     /locus_tag="PLK75_01660"
     CDS             4412..6469
                     /locus_tag="PLK75_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37982.1"
                     /translation="MNKPIVISLIAIVMITVFVNKQSVAAVSSKYISDMTVAKVINEL
                     KAKYPEQDFRIERGVNQVAALWFQTDGSETDFTEFCLANYIGNSDELDVVFSKLERNL
                     EVLYGSFNHIVIGLMEPLHLDMGSIHPIDEMFGAYSPGAHLSDDFFSNKIAFIVMLNF
                     PAYTLTEKKAMGTNWTPKQWAHARMGDMFTQRIPAELILNVSTATTNADTYISEYNIM
                     MGNLRNNKNESLFPVDMKLISHWGLRDELKSNYNNTNGFEKQMIIYNVMLHIINQSIP
                     AMVINNADVIWNPISNTVWKNNQEIAVQREPDTRYQHLLRVFESMKAIDEFTPMYPTY
                     IERAFDGGMELSLEETEALFVSYISSPVIKDVAKLIQKRLGRKLQPFDIWYDGFKARS
                     SINEDELSAITRKRYPNREAFAKELPLILVKLGWDKTTASWISSKISVDPSRGAGHAW
                     GAESKEDVAHLRTRIGANGMDYKGYNIAIHEFGHCVEQTITLHKVPYYMLHGVPNTAF
                     TEAVAFMFQAKDLQLLGVENTADAKEIEALTALDNCWSVYEIMGVSLVDQRVWKWMYQ
                     HPQATPEELRIAVTTIAIDVWNQYYAPVFGVKDSPILAIYSHMIDAPLYLSNYPVGHI
                     IEFQIEDYVEGKDFASEITRMFTQGRIIPQVWMKNAVGSEIDAKPCLDDAAKAVDFLK
                     KKK"
     gene            6706..7122
                     /locus_tag="PLK75_01665"
     CDS             6706..7122
                     /locus_tag="PLK75_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY37983.1"
                     /translation="MLQLDRYKNKFTGKTILIADDVEDVLFYYKALLQFTGIRIMEAR
                     SGNEVISICRHKPDIDLIMLDIQMPNGDGMYTLERLKEMHSRAAVVAQTAHALPTDKI
                     KYMKLGFDDYICKPLRSETLFQILEDHLTVTESERK"
     gene            7174..7764
                     /locus_tag="PLK75_01670"
     CDS             7174..7764
                     /locus_tag="PLK75_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOY37984.1"
                     /translation="MDDKRIFSLIKQGNEDAFNKLFRAYYSKLCHYAVMLGCCGEDAE
                     EIVQELFTKLWMNRQKTEIDISAKSYLYKSTRNAVINLVEHQRIKQKYAGEEILAKTE
                     FFDVNPLLVAELQEHIQSAINKLSEGRKQIFILSRSDGLSYQEIAEKLNISPKTVENQ
                     MGHALKQLREELKDYLPMILVSGLAYFFCEWGYLCT"
     gene            7779..8756
                     /locus_tag="PLK75_01675"
     CDS             7779..8756
                     /locus_tag="PLK75_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOY37985.1"
                     /translation="MPEINTYDYELLAAFIVGTLTLEQQKAVQERISSEPDFDKLYRQ
                     LLFIYSAYPAINTFDTEKALRKVHEIAGKKKSKIHITPRTLFYSAAAAVIILIALTLA
                     NSLFLTGNKQINYILVASTDSVHVTSLSDGSTATLNKNSILRYESGFENSELRRVELE
                     GEAFFEVKHDATKPFVVVTQAAEVLVTGTAFSVNDFSGKEVVVWVEQGSVSVTSATSN
                     QTIHLNPGEKAICNKTTGSISKTYGSGDETFWKSRFLSFTSTPLSDACITLEKNYGCS
                     IEITGSKLHELTITASFENEEPEHILEVIAITLGLQLEASNNHYRLYAE"
     gene            8746..10572
                     /locus_tag="PLK75_01680"
     CDS             8746..10572
                     /locus_tag="PLK75_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37986.1"
                     /translation="MPNRFTARIQLLILILLTPAFQSFSQNAYLSKKIDIDLKNVSQA
                     KALSEIEIRGGCRFSYGQNVIKADSMVSLYARQQSIQHILITLLGPSIEMIESGNHII
                     LRRKNEPKPLFTGVVVNEKTKYAVANAKISENNRSKTRTDRNGKFSVMLSEYPCFIRV
                     SANGYRDTVFQVTETYKNVSVSLIPDSKVPEVLFKAPSLAPEQLGTGSGSSGDMKSAF
                     EIRPLHDSMTYQSLEKKDFQISVLPFVGSDFAASRDYKYGTSLNLPCGYTGGVSGVEI
                     GLLMNIVRQNVIGFQFGGLLNVVNGSVSGTQISIGSNLVFNNFSGIQAAGVYNYCRNE
                     FNGVQFSLIHNRLSGASGGVQFSLISNLTEADFSGTQVSGVCTINRGNYVASQVALGA
                     NVTDGTCGVLQLSVFSNTADTVEGVQLALVNRCKKCHGVQVGLINVSEDNDGIAIGVL
                     SVVKNGYRAVELSYSELNNVNFCFKSGLRKFYNIINTGISPQSGNWRMNLGYGIGSSV
                     EFGKRWMGNCDLVINHVSEKNSFVKDLNLWLLLKPQIGVNIANTCVLFTGASLNLSIS
                     RFRNPYTNEFLTELSPEYSIFSKEHNNTKYSMWVGYSIGIRF"
     gene            10644..11312
                     /locus_tag="PLK75_01685"
     CDS             10644..11312
                     /locus_tag="PLK75_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37987.1"
                     /translation="MRKIIFTFGFLAAITAAYAQNDSLNRSRNNSEIKTLFDSDGITS
                     HGIYAGCSMYYTQIAGRNAFVGGIKGAWVIDHVFEVGGELNGFLSEPLPDVTLSNSYY
                     LYSGAYGGLLFATNLFPQKAINLAIPISIGAGGVAYVGNYYYFSEDYEYYPESYYAYF
                     YVQPGLELQFNMTQHFRLAIHGSYRYTSNIYLEYSGGTGQIIGNNNLMNGFNAGITLK
                     IGKF"
     gene            11327..12169
                     /locus_tag="PLK75_01690"
     CDS             11327..12169
                     /locus_tag="PLK75_01690"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOY37988.1"
                     /translation="MKRIVLISVTVALLAGMTACYFPHDYIRGVGQLTSIEKDTRGVN
                     GIEINDIIDVVLVKSDTAKIIIEAQENIIDLVSTEVDNGICKVEFVRGYNVRPTLPVT
                     VYIYTNIISSIEIDGSGDLTTNGIFDTLGNLEIDINGSGNVDFAWLEANDIYLEIDGS
                     GDINVTGNGQNMSTTIDGSGNLHMGTELAGTSFRINGSGDIFISGWSASNILHIDGSG
                     DFYGFDLETETTSVFIDGSGNAEVWATENLLVEIDGSGDVVYRGNPNINSGGSGSGNL
                     INGN"
     gene            complement(12335..12991)
                     /locus_tag="PLK75_01695"
     CDS             complement(12335..12991)
                     /locus_tag="PLK75_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOY37989.1"
                     /translation="MNLQRIFGFLNELQQNNNREWFRQNEAAYRQSVSLFEKFVQQLV
                     IGINEFDPSIGHPEAKDCIFRIYRDVRFSHNKEPYKTNFGAYIAEGGKKSIKAGYYVH
                     LQPGASFAGGGIYMPAPEILKAIRSEIYYNTEEFKRIMGAKEFTTYFSGIYGDKLKTC
                     PKDFPKDFPDASLLVYKDYTVIHSFSDHEMFSENITDEILKVFRAQHPFNDFLNNALE
                     "
     gene            13138..14538
                     /locus_tag="PLK75_01700"
     CDS             13138..14538
                     /locus_tag="PLK75_01700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY37990.1"
                     /translation="MQIKFIGAAQEVTGSKHLITTKSGKKILLDCGMFQGKGLDTDRM
                     NRELGFDPKEIDHVILSHAHIDHSGLIPYIYRLGFSGSVVCTSATRNLCAFMLPDSGF
                     IQEHDMKWFNKKRAKQGLPPLEAIYKKDDAVQCMKLFIAVDYDRKFRIDENITVRFTN
                     SGHMLGSAVVNLEIKENGKITRIAYTGDIGRPYNRIVKPPAPFPQADILITEATYGDR
                     THNTQNESEETLLNIVIDTCVRKGGKLIIPSFSVGRTQEIVYSLNNFYNEGKLPAIDV
                     YVDSPLSVNITEVFRIHPECFNDDVLEVMETDDNPFGFRNLKYIKNVAESKKLNTTEK
                     PCVIISASGMMEAGRVKHHLANSISNPRNTVLAVGYCAPETLGAKILRGDKVVSIFGT
                     EYPVLADVYRLESFSGHGDYKEMAQFIKCQQPEKLQQIILVHGEYDAQQHYSEYLHSM
                     GYKNILIPQKGVEITV"
     gene            14545..15708
                     /locus_tag="PLK75_01705"
     CDS             14545..15708
                     /locus_tag="PLK75_01705"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY37991.1"
                     /translation="MKISLVVVILSMLFLVSCKNNEIPSMNIVKEGDVGWNSKEACTI
                     EYCTGGDTLILKAAIKCRGGVSSKYFKHSYSLELDDKFSVAGLPRDDDWILNANYIDK
                     TFMRHKISYDLFREMSPNNAASQCAYVNLLLNGKPMGLYVLMQEINAGMLDLDRKDPM
                     AMLFKDPPVFYADKITSVQDSLNYYQQKYPKIQQCDKTSTIEEFKNFLFFSSDSAFAQ
                     NVSKWVDIDNVIDWHIVLLFSNNSDGIMKNFYLYKPDSHTPFRFAIWDYDHSYGRDGD
                     NEPHHANQELNWQRCILLKRLYQIPATGYTQMLKDRWAYLRKHDIISVAHFKELMDEN
                     DKAISQAVINNFALWPVNEKWYFDANTYQQETALMLKFVEERIPMLDAYIEKL"
     gene            complement(15766..16650)
                     /locus_tag="PLK75_01710"
     CDS             complement(15766..16650)
                     /locus_tag="PLK75_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37992.1"
                     /translation="MRLSSVTVMNRPSSTLKTFWVSIILVFGYCNINAQMFIAGDTLN
                     PEITYVNIPDTILPIVIKGFFNFDIDIDSDNINDIRFHREHSSSPSYSSETFSVQSLN
                     SVQFVTQPGSSDAEPLLLGTEIDNALHWNDNYDGACFYYYFDSHIPPPWGPSSTSHGT
                     CTLPDTYIGFRKIYASDTLYGWFFLDLPDPYRIKSYAIDKKLGSGIPNQASTTNTFII
                     FPNPASDFIDIMNNNKDFNTFHLSIINSTGQIVLKTNIELVDKCSFDLRNYTDGLYII
                     ILQNENLKFSSKFVIRKL"
     gene            complement(16657..16998)
                     /locus_tag="PLK75_01715"
     CDS             complement(16657..16998)
                     /locus_tag="PLK75_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37993.1"
                     /translation="MNTIKLAVVLIVIGIFTVSCEGPIYFNVTVVDKITQQPIDSVLV
                     KVIVKVGDKEKSAYNLQGYTDTAGNFIRDEMIGYGLSLRQWDFYMEYEKEGYIKKTEI
                     NHTDGLVELEQ"
     gene            complement(17243..19345)
                     /locus_tag="PLK75_01720"
     CDS             complement(17243..19345)
                     /locus_tag="PLK75_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY37994.1"
                     /translation="MRSIALLTLLQFFFMLQIFSQNDQWQTFSRADVVYAIAEDGNYC
                     WIGTSTGLVRVDQTTHAKVHYNKSNSPLPDNIVKSIAVDNQSNKWIGTCYGGVARYDG
                     VNWETFTTDNSPLPDNSINTIFCDNNNGVWIGTNYGGIAYLNNGTWIIYNQANSDLPD
                     NSVQYITSDNGGQLGIATLGGGIATFDGVTFTVFNDNNSAPINNSLTIAFEAETMWVG
                     TLDNGIAVFDGTSWVNYNEDNSILVQNTIRSIEILKNGDVWIGSDYGLYIINDDTWIY
                     CTTCNSGIPAGALYDICQTSDEKIWVGSSLQGLKIYNDPYWNPVPISESSIASNYQYR
                     VCVDNAGVVWTGARDGEINGFYSDQCTYLYDLGEWINALAVDNNGNLWAAATGAYSFV
                     QNNTNYYNESNSGLFDNNIRSFAFDSQNNLWCGSLHGGLQMFNGNQWVSYNTENSEIP
                     GNWVMDVAVDNNNTIWMTSQHNGLVKFDGQQWTQYNESNSNIPGNYTDYIAIDANNVI
                     WVAGEGLSRFDGTSWTNYNTSNSGLPENTITCLGIDRKGNIWAGTYEHGIAKFNYNTN
                     EWVVYNAANSGLPLNSVTDIAFDASNNKWITTFGGGLSKLISNENTEDIENISQTLKE
                     IRIYPNPVTDLLHVETSNEAQIAVVEVYSIAGVRLMTINPAGYNCVLNMDNWDSGTYL
                     LKIKTANRVFTERFVKSE"
     gene            complement(19662..19928)
                     /locus_tag="PLK75_01725"
     CDS             complement(19662..19928)
                     /locus_tag="PLK75_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY37995.1"
                     /translation="MNTKKLENLVTKTGVGLLFFLTLVGIVFVFDVAFNLDLFSNAMK
                     QAAGILLGIVCIFVVSAVLVSLMLNVSRIANSIEAMANKQVDKM"
     gene            complement(20482..22026)
                     /locus_tag="PLK75_01730"
     CDS             complement(20482..22026)
                     /locus_tag="PLK75_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY37996.1"
                     /translation="MRTSKLCKSGIMLLVAVALRCVEVAAQDQITFTWHATGEKIIYK
                     TQATNAEEFYVNWGDGSAIDTETGLGYDGGAYTNVQLTHNYAEEGEYTVTISAGENCR
                     FYYFDCSGFYNSETSTIVNNQISSLQITDCNELEQLYCYYNQLTTLDVSTCSSLWLVY
                     CSENLLTSVTCSPAVQRFYCHVNQLSDLDLSTYTELTMLMCSHNQLTELNLNSCPNLQ
                     ALSCDYNQLSTIDISSCPNLQEFCCNFNLLQTLDVSNSQNLQTFICNNNQIHTLNTSG
                     CTSLHILSCGNNVLTELDLTDYTSLTEVSCTGNELSALNINGCSSSLRVYCNENKLQL
                     SDLFEISELIDDANNKWLGTQYISTTADTGDELFADQSVFSDIFTNYSVNPDGYHTVV
                     EGKLIFNAAGDYTVVMTNDAIIAYPDFSTQVIAEITVNSGVNINSHSLSDMLLFPNPT
                     HDYFMIDPTGVATVKLYDMLGKEVLSQNANGKTEINISHLPNGMYSVQIISAERIVAN
                     SKILKY"
     gene            22546..23637
                     /locus_tag="PLK75_01735"
     CDS             22546..23637
                     /locus_tag="PLK75_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOY37997.1"
                     /translation="MKNSFRILLVVLTTALAFTIGCKDPEEETEATFNLKITDARVPQ
                     AATKDGDAPVLISDQLTKCEVTYADIELKKDDGEYISLMTSPTTVDLRDFQGTLHSLL
                     SISIPVGSYTHLKVTVSGVSTTYQNNNYTASVDGGATVTLNNIPGTFTEAQGVPDVFS
                     AGAISYEVPLAFTLINSEDVESVQLQFDVDGSVYIVPFTYQSFTWNFAALHTYFSLGY
                     VFEQGIQQIRHSPPLGITIVSANDVDYYGIHTFVDFDAHGGTINSHTSQHVFRGSDGS
                     LLVDAEAMTINNNVLSPNTVNATGESDIRADETFHYTAILNNLAGQGYTLLSGHTYYF
                     SLRKTWNITTNGTTYDLTRICEPIPVTIP"
     gene            complement(23653..24765)
                     /locus_tag="PLK75_01740"
     CDS             complement(23653..24765)
                     /locus_tag="PLK75_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY37998.1"
                     /translation="MRIILFILQKEFRQIFRNRTMLPIIFVMPLIQTLVLVYAATLEI
                     KNAPVSIIDLDHSQLSKQIISKFDASPFFTVNDGQYSHDYIAGELKNDNSKAVIIIPA
                     GFEKDLKTGAATGLQMNINAIDGQGAGLISIYAERIITAAVNQAAGTKQEAVIKPAAV
                     DIRPRFLYNETLNFKYFMLPGILTILVTMIGSFLTALNLIREKEMGTIEQINVSPVGK
                     FQFLIGKILPFWVIGLFDLSLGIGIGMLLFGLPFAGSVLTLFVFTCVYLFTAIGLGIL
                     VTDFARTQQQVMFTIFFFFLLFILMSGVFTSVESMPHWAQEVNRINPVFYFMKGIRMI
                     MLKGSTLADIATVIGSLGALGTVIMAVAVWQYRKTT"
     gene            complement(24765..25871)
                     /locus_tag="PLK75_01745"
     CDS             complement(24765..25871)
                     /locus_tag="PLK75_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY37999.1"
                     /translation="MKRFAGFLIKEFIHIFRDIRTMMILFAIPVFQILIFGYVIKNEI
                     QDVKVGVLDFSGSPHAVEMINRIDASADFEVVSRYYSYPEAEEAFRRSEVKEVIVIGS
                     GFDKDLHSSSGATIQLMLDASDPNMANLINSYTTAMIMKYIAGLNNNGISLHRPSVET
                     RMWYNENLKSSYMFVPGTMALILMLITAMMSSISIVREKENGTMEVLLASPLNRWQII
                     FGKVTPYILISVVNACSVIAIGNLVFGVPIHGSLWLLFGETLLFIFLALCFGIFISSA
                     VKSQMMAMFISMFVLMLPTLLLSGFIFPIENMPVVLQWLSVIIPAKYFLLIIKMIMLK
                     GGGLADVWKPTLVLLGMSLFFLGMSIKKFKARLE"
     gene            complement(25878..26612)
                     /locus_tag="PLK75_01750"
     CDS             complement(25878..26612)
                     /locus_tag="PLK75_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY38000.1"
                     /translation="MKTDREIIIKAENLVKKFGNFIANDNLNFEVYKGEIFGFLGANG
                     AGKTTAIKILCGLSYPTSGNIMVAGFDVYRQREKIKKNIGYMSQKFSLYEDLTIRENI
                     RFFAGIYGLSRDYIRKRTEELLEELRITHLGDSLIASLPLGWRQKLAFSVAVFHQPRI
                     VFLDEPTSGVDPVTRRQFWDMIGKAAAGGITVFITTHYMDEAEYCDRVSIMVDGRIDA
                     LDTPVNLKAAFGVERVEDVFLKLARK"
     gene            complement(26609..27517)
                     /locus_tag="PLK75_01755"
     CDS             complement(26609..27517)
                     /locus_tag="PLK75_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY38001.1"
                     /translation="MAYIEVAEVSKNYKKVEALKNLSFSIQQNEFFGLIGPDGAGKTT
                     LLRIMASLLLPDTGSVTLNGLDIKVDYRSVRKILGYMPGRFSLYQDLSVEENLNFFAT
                     VFGTTIHENYHLIRDIYVQIEPFRTRRAGKLSGGMKQKLALCCALIHKPEILILDEPT
                     TGVDAVSRKEFWEMLKRLQSQGLTIIVSTPYMEDAGKCDRVALMQGGAMMAIGSTASI
                     SSSFGDALFVVTDSDAFRCLESLRQIPEVLSAYAFGKEIHVAAPHGKLSVDEIANHLN
                     SRGFENFNVEQGEASIEDVFLQLMTK"
     gene            complement(27520..28425)
                     /locus_tag="PLK75_01760"
     CDS             complement(27520..28425)
                     /locus_tag="PLK75_01760"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOY38002.1"
                     /translation="MKNNATIVMLCFGAAAITMLISCGGNGEKADAYGNFETESTVIS
                     AETSGKIIFMPVEDGKQIRKGDTIAVVDTAILVLQRKQLITQKDAVRVKFDNIINQVA
                     VAEEQLSSLLKEQQRVADLLKNNVATQKQYDDITSQVNVSRKQIESLKSQNAGVFAEL
                     DVIDTQIAILNEQLQRSVIISPLNGVVLEKYMQANEMAVQGKPICEIADMSTLTLRAY
                     VSAVQLDNIALGDTVEVLYDKTVTEKATCRGTIAWISDEAEFTPKIIQTHEERVNLVY
                     AVKIYVPNTGELKIGMPAEVNFNNK"
     gene            complement(28415..29695)
                     /locus_tag="PLK75_01765"
     CDS             complement(28415..29695)
                     /locus_tag="PLK75_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY38003.1"
                     /translation="MKKLLIIGLFIAVLFPTMGQKNVLSLDSCLNAAERNNPAIRLFG
                     LTEENYIFKEKSLKTSWYPSLTLNAQATWQNEVTNIDIDLPIMPQSLTDISKDQYRIS
                     LDLRQNIWDGGVTAAMLDYERAAVVSETEKIKKDMFHFKEQIVDLYFAILKLQSQQEV
                     LMVKKWQLLLKVEDLNKAAASGVVLKETIDMLNAEELVVEQKIIELDYEIAASKKMLG
                     RLMDVPISEDAMLLLPSPELSLQTGITTSDETYLHAMQNQLSSSDKMLKTSLMPKVFA
                     FSQLGYGRPGLNMLSNEFEPFVIVGAKMVWTPWDWGKSKSDRQQIQVQQSIIDTQIEQ
                     INYLQQSQLDAQMQRIEKCEKLMEKDEEIITLKHNITEAVSLRLNCGTATSTDYLNAL
                     NDEQAAQGNYNLHIIMLAEAKVKYNLLKGIMYEK"
     gene            complement(29692..30312)
                     /locus_tag="PLK75_01770"
     CDS             complement(29692..30312)
                     /locus_tag="PLK75_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY38004.1"
                     /translation="MKSLEINESAEQRIYDAAVAVFLERGYDGARMQEIADRAGINKS
                     LLHYYYRSKDRLFKKVFADTVGAMFESISGVFQEEIPFEEKLKKFCTAYLDFLIQRPQ
                     LPLFLLTEIKLHPQLIAETMTDLKLKARFALLKSLVHQSAEKNEIHVQSEVHLMINII
                     SMLIFPVVARNMITMLLDLQPEEYEMLLNERKDRVPEFVLKSIFAS"
     gene            30542..32506
                     /locus_tag="PLK75_01775"
     CDS             30542..32506
                     /locus_tag="PLK75_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY38005.1"
                     /translation="MAIPVVASAQDKKLKKADEIFATGEYYRALELYQGLIRKVKDKR
                     VKQELYFKSGECYFYINNFKKARANYKKAVKHPELGVDAYYKLGLTSKLTGDYEQAIL
                     DFKEALSIYPGDSLSQQGLESCELSVKWMQERSRFEAEKFKEANSRENDFCPTVVENN
                     GYPHLYFSSTRKEAMGKKTSGITGEKFSDLFVVKFGKQGKWSKVNALDSINTIYDEGS
                     ATFVNDGRDLFYTSCVKEKGKKLGCQVYKARKESIEMGNNIDVQWMNPERVDILPDSI
                     SFGHPAFSPDGTIMYFSSRKDGGFGGADLWYSEQEGSTWSKPKNMGPIINTKGDELFP
                     FMRADGVLYFSSDRHPSMGGLDIFKAYKDENKKWVVVNMKPPFNSNGNDFGLYYFAGE
                     EKGYFTSDRKGSQKEDIYSFVKPILKIEFKGQVFDLDTKQIADSAIITLYCINGPTKS
                     DTISLAEEGRFQFELKENSEYSFSVEKEGYFRGRGRLRTDTLDFDYTFEEMIYIENYN
                     KTVEIPNIEFEFGKYSITEASKSSLNSLIKLMQDNANLVIELSAHTDMVGTDESNMEL
                     SIKRIESVKQYLIEKGINSERIKGVGYGESKPKQIRTYTPEHPWLQIGTVLDETYILS
                     LDKEKQEIANQMNRRIEFRVISNKYEPGLD"
     gene            32654..33424
                     /locus_tag="PLK75_01780"
     CDS             32654..33424
                     /locus_tag="PLK75_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38006.1"
                     /translation="MKTLATILLSFIMILLWTYPSSAQVKNGYHDKLAALYEEGEYLD
                     CAFKAERMLMKDEYLSDPEVYLYLAISYNKIYLMTKEDPTIIQDEPEYAKAYDNALKY
                     AADSKKKDKKAKEFFPDNNDLLEEIVYTGLPIAEQYIFEKKYSKALALYRKFLKLIDN
                     LHISFIKGVMDLYTLDKATANEEFKKVFAAIEQNSLTTNDNTKYLMKTGFLMYYDFLL
                     QEDSVANADSAKHVLRYAVRLFPEDREINQRFFPSSKQ"
     gene            33430..34179
                     /locus_tag="PLK75_01785"
     CDS             33430..34179
                     /locus_tag="PLK75_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOY38007.1"
                     /translation="MIGIIGGTGLERPDFLQFTSELEINTAFGSPSSPVKLAEFGGKK
                     IAFISRHGENHNITPTHVNNAANILALQQTGCNCIIATTACGSLREEIGRGDLVIVDQ
                     FIDFTRHRRVSFYDSFDNGEMKHTAMAEPFDYNLRKMLAEAARQSGINVHTSGTVITI
                     EGPRFSTRAESNMFRIWGADIINMSTAPEAILANEAAIPYAVVALSTDYDCWKTNEEP
                     VSIEEVIHTFRTNIPRLIQLLSTFISGYIER"
     gene            34198..34974
                     /locus_tag="PLK75_01790"
     CDS             34198..34974
                     /locus_tag="PLK75_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOY38008.1"
                     /translation="MKIIRKDMHESGNLTAYEVIRIIGSRPLFLNEHLDRLQRSLFDI
                     DKDCILPVTLRGEFIAFVQKTGVVDGNIRIDAWFTETTQYRFYQIPHHYPTDQEFCNG
                     VSAVTLIAERNNPESKIWNKELRSNANSLIESSGVWEVLLVNQKGNITEGSRSNFFAI
                     SGSTIVSAPKEQILGGITRKYVLEAIRSADNVDYIEQDIPLHSIKNFDAAFITGTSIK
                     VLPLKSIDGQYLNVNNLILSQIISNFDHIMQRELNRNYEM"
     gene            34964..35557
                     /locus_tag="PLK75_01795"
     CDS             34964..35557
                     /locus_tag="PLK75_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HOY38009.1"
                     /translation="MKCNKTSIVVVLLVLAFCTGATAQDWKNCYDYPGIKISYISDTV
                     KNVYKVRVSSLSSITMNELYKRLLDVKKYPDYVTNCIYAETLKKYSDSCFIYYTITEL
                     PFPLKKRDIVVKLTSKKSAGAFTIVTEVFNNFLEEKQGYDRVKGYKAIWFVEETNAGL
                     EIKLSMELSVPQIVPAWLRYQIIVKGPVETYLRFTHH"
     gene            35628..35867
                     /gene="yidD"
                     /locus_tag="PLK75_01800"
                     /pseudo
     CDS             35628..35867
                     /gene="yidD"
                     /locus_tag="PLK75_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006043716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            36012..36800
                     /locus_tag="PLK75_01805"
     CDS             36012..36800
                     /locus_tag="PLK75_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOY38010.1"
                     /translation="MLSGCHKNTEQPPPPTATAYDIILVIGQSNTHYGEGYDTDLDCP
                     ESGIWQLGRFGANNFKIIEASEPLEHYTSQPGFIGFALSFAKYYKKTFLHTTRQVLII
                     PCGKGGTGFTDKSWNPGDPLYEDAVSRTNFIIRNYPNSKLVAILWHQGERDEFNQNYK
                     EALDSMIIRIRMDISGCSASTPFILGGMVPYWTNKAQSRVEINNIIAGTPNRIQNTGY
                     ADPRKPYIIEKPDNSYSEIHFDAAGQRELGRRYFVVFSSMVRPQ"
     gene            36910..38529
                     /locus_tag="PLK75_01810"
     CDS             36910..38529
                     /locus_tag="PLK75_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOY38011.1"
                     /translation="MKFQFFMFATCIATGLFAQTQVNPKIMEVTVFSNAAEIKSIASV
                     NAGAGITSIVCLDISGNMQVNSLKLNPSEGVEITGFSVESYYLLPETLIPEYKRMNDS
                     ITMLNNTIITLRNKQEAYQAEKNLLMANQSIGGDQNGVTLQELKSISDFYRERLLAIN
                     NELSKLTDEISKLEKQMSEISNRMTIASYKHESSRKKVYIDISSESAAKVEIELRYLV
                     GKCGWAATYDLIAEDINMPVKLKYKANVLNNSGIDWNNVQLILSTLDPLKGASAPVLT
                     PWYLDFTADEYNYQDQYRYSRSSNEIEYKSEQMTPGQIMDPNQAYDNISVSELSTEFI
                     INRKYSIPSDQKPFKIDITEYDLNAEYKYVTIPKLETKAYLLARITGWEKYELIDGDM
                     NVYYSNSYIGVSRLNTNTVNDTLDLSLGRDNKIMVTRIKMEDFSSKSFLGNNRKESNT
                     YQIQIKNNKGTPVNIEVVDQIPVSQESEIEVGVNEISAGAYNPITGLVKWNTELKPAT
                     SQKFTISYWVKYPKNKPVQMSRKRAVAAPRF"
     gene            38654..39514
                     /gene="lgt"
                     /locus_tag="PLK75_01815"
     CDS             38654..39514
                     /gene="lgt"
                     /locus_tag="PLK75_01815"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOY38012.1"
                     /translation="MNLLLQVVWDVDPIMVDLGLFAVRYYSLLFALGFVISYFIIKKI
                     FKKEGTPIEELDRLLIYVVVGGLVGARIIHCVFYEADYFLSGFFPFLEIFFPVSFSPE
                     FEFIGYQGLASHGGALGIIIAILIFKNKSKNKSFFYLIDRVAIPTGFAGACIRLGNLF
                     NSEIYGEKTSLPWGFVFVKNNESEPMHPTQLYEATFYIITSIVLLLLYRVERFRNARG
                     FLLGMFLMMVFGSRLLIEFIKHIQVGFEENLALNMGQMLSIPFILAGGFLVWYSLKMD
                     GHTVDVKKNN"
     gene            39574..40653
                     /locus_tag="PLK75_01820"
     CDS             39574..40653
                     /locus_tag="PLK75_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOY38013.1"
                     /translation="MWRLTKELFKRYIRAILLLTGVFLLILLGILAIEFGAGHSTIHN
                     YFEAIWYGFVTIAGVGYGDYTPVTTGGRILGMLLIIGSLLLVGIFTGQITNEIRKIME
                     DKKMGIYGTKMTGHFVIIGWDSFASNVAEWVLDAGKDLAIITNNPADIDLIYNRFGNK
                     KVFALFSDFDKPESFDLVNIDKSASVFINFKEDSETLVHLINIRKKYPRLNYVASLNN
                     SQLKDTFHAAGITFSVSNNEVASKLVASYIFEPDVANFTEDIMASANMNDDYDMVEYQ
                     VFSNNPYANKDYYEAFFDLKTKYNCVLLGISRKIGEKYTIIKNPKKGEIIRNADFLIM
                     LADGSTKPQIEQLFGTKEGRCVLKS"
     gene            complement(40650..40829)
                     /locus_tag="PLK75_01825"
     CDS             complement(40650..40829)
                     /locus_tag="PLK75_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38014.1"
                     /translation="MIQHSTLLVLYNNSAEPEKIVIDCNQIADFNLGNELKNIDFEPD
                     EKVIDNLILFLKNKD"
     gene            40940..41695
                     /locus_tag="PLK75_01830"
     CDS             40940..41695
                     /locus_tag="PLK75_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38015.1"
                     /translation="MRNWIGEHINAILGTILAHLIIVFIALVLKINTSVVHSETPIII
                     DTELLAEMQNIPEENSSEVNDMVNVDEYLSKLTSASSNYSGMNRSANDNLHDSKNLSV
                     DQIRQMYEEEILREKYGDDYESRYEESDYTYTTPYAIPDKYRAENTQGQQIDNTNKPC
                     LVKAVLDDPTRATRYLHIPVFTCQGSGVIEIAIVISPEGKVINSKLLSTNTSNDQDCL
                     VEAALTASRKSRFGIITSKQNQNGKIIYNFVNQ"
     gene            41757..43379
                     /locus_tag="PLK75_01835"
     CDS             41757..43379
                     /locus_tag="PLK75_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY38016.1"
                     /translation="MIQEKLVSYFDNSIRENWNKTALRDYDNGEISYAKTGNTILKLH
                     DVLRRSGIVEGDKIAVIGKNSANWACVYIAAVSYGAVIVPLLQDFTAKEVYNLVNHSE
                     SKLLFASDFIFNKLNLQEFTLLKSIYQLDNFELLFDADQKSIRFNNALPEVSQADFAL
                     HIPENSLLAAILYTSGTSGNPKGVMLAHNALAANIRYARANMPLQPGDRIVSFLPLAH
                     SYGCAFEFLYPFSIGCTITFLGRLPAPNIVVKAFNEIKPRLILSVPLVIEKVYKTKIK
                     PKLSKPGIKLMLKIPGLRQIVYRKIRAGLSASFGNEFMEVVIGGAKLNREVEIFLRKI
                     NFNFSIGYGMTECGPLITYDGWKTTRTFSAGKVIDCLEMKIDSDNPSKRVGEILLRGE
                     NVMLGYYKNKDETEKTFTEDGWMKTGDLGLIDEDGYLYIKGRSKSMILGASGKNIYPE
                     EVEAKLQNLPLVSECIVVGRQNQIVAMVYPDHAIIEERALNKEQLETIFSNYQKLVNK
                     DLPGYMQIQKIVLREEEFEKTPKKSIKRYLYE"
     gene            complement(43389..43802)
                     /locus_tag="PLK75_01840"
     CDS             complement(43389..43802)
                     /locus_tag="PLK75_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38017.1"
                     /translation="MRKIYLILTMFALSLMALAQQNRPDQIPYQRFDSLKYTDGITQI
                     NVYVHLDCGRCKDAVELYETAGIPFKIIDLDNEENNVVLDKKITASISVADRGKGYSV
                     RYPVIEINNDVYFCIENHYYFNQALVRYFERQKKK"
     gene            complement(43842..46280)
                     /locus_tag="PLK75_01845"
     CDS             complement(43842..46280)
                     /locus_tag="PLK75_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38018.1"
                     /translation="MKRACFFSLLFIISLFSYGQYATLVGYVIDDENHAVQFANVSVS
                     PGGLGTATDKNGFFTLQIPANKEYTITITFIGYQKYSESITLSPASVTEKNIVLHKEV
                     NILPSIDVADKNERRTNLIRLNPVIASNLPTANGMEDVIKTLPGVVSNNELSSQYSVR
                     GGNFDENLVYVNDIEIYRPLLIRSGQQEGMSFINSDMVSSVLFSSGGFEARYGDKISS
                     VLDITYRKPVEWKASLTASFLGVNAYFEGLSKDRRFRHITGFRYKTTKYLLGSMDTEA
                     LFDPRFTDFQTYLSYDLTEKFEISFLGNYSNNYYGFIPEDRETSWGTLDEALKIKMYF
                     EGQEVDKFVTLTGALTGKYKLNKNVDMKFIASAFRTSEEETYDILGQYYLNELDKELG
                     SDNLGDSISNIGVGSFLNHARNYLDGYVISFSYKGDYRHNDNLMSWGACYKHEIFDYS
                     VKEWQMIDSAGYSLPYTDSLVMLFFTDTSVVGMASERVEAYFQNTYSFKAGEGEIGLN
                     AGARGSYWSFNNQALISPRMVVSYDPKWKRDFVFRFSTGLYQQPPFFKEIRRLDGTIN
                     EDIKAQSSIQAVISSDYQFLAWNRPFKLVTEVYYKYLYNIIPYDVDNVRIKYYGENMA
                     HGYAVGIEAKINGEFVKGTESWLSMSLMKTMEDIDNDFYTVESQSGQTDTIFPGYIRR
                     PTDQRFNFAIYFHDYLPNNPTWQAHLNLLFGTGFSFGPPNSQRFQATGKIPPYRRVDL
                     GISKQLIGNKPLSESNPFRFIKDFWITLEVFNLLDINNTISHIWVSDIYGRMYAVPNY
                     LTGRRLNLKIKINF"
     gene            complement(46339..>47396)
                     /locus_tag="PLK75_01850"
     CDS             complement(46339..>47396)
                     /locus_tag="PLK75_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOY38019.1"
                     /translation="IYTFFNNRIALVSFWMLMLLIGFFAPNSFEFVFIQITAGMVVLF
                     SLRHIQRRFHLFVSVLLVLVSYVVAYSGFTIINGIGFSEINWETYAWFGGNAFFLLTL
                     FPLMYLYERIFGFISDVTLLELSDTNNPALRTLSEKAPGTFQHSMQVANLAESVIRKI
                     GGSALLVRTGALYHDIGKTEIPEYFIENQHGVNIHDNLTFDESASIIINHVNKGLELA
                     KKYRIPSTIVSFIATHHGTTMTKYFYNSYINQHNAINIDPAKFTYPGPKPETIENAVI
                     MMADAVEASSRSLKSYTRETIAALVNKIIDGQVAEGQFEKVNLTFRNISETKEIFIEK
                     LMNIYHTRIEYPEINKA"
BASE COUNT        14561 a         9823 c         9611 g        13401 t
ORIGIN      
        1 aaattatggt cagtggccgg tgaacgatgg ctcaggagat attctcgttg atgacctgat
       61 atttgattac acacccgttc aaggtaattc gtacagggtt accggcgtgt tggatttttc
      121 tttcgctgaa tggaaaattc aacccagatc ggcagaggat attgttgacc ttggtgtaaa
      181 cacacaacct actttataca taaactctcc cgaaaacggt tcaaccattt acacagcttc
      241 taccaatttt ggctttaccg tttctaactt tacactgggc actgatggta aggtagagta
      301 tatactcgat agtcaaacac cggtttatgt aaccacttcg ccctcagcaa ttaccggact
      361 tactaacggt gaacacacta ttgttgcgca attggttgat atgtcggaaa acccgctaaa
      421 cccagctgtt acagctacga taactttcac agttaaccta tcaggaccaa cattgacgtc
      481 aatttacgac attcagtata ccgatcaggc ttcgggtgct tctcctctgg aaaaccagac
      541 tgtatcgatt aaaggcgttg taagtgctgt tttcaatgga acaccttatg gcgaaggata
      601 ctatttgcag caaggcagcg gagcatggaa cggactcttt atttatgatt tgaataacac
      661 ccctgctctt ggagattcgg tgcttgtaac gggcaaggtt tcggaatatt atgaaatgac
      721 cgaactcaca aatattaccc ttttccaagt aaccggaata ggcggcacaa tagctaaccc
      781 aacggtggta agcacactcg acgccaacac cgaaatgtat gaaagtgtgc ttttaaaagt
      841 gatgaatgct gagtgcactg cagtgcaaaa cacatacggt caatggactg ttgacgacgg
      901 aacaggtgca ttattatgtc aggacaatgg agcatttagc tttgaagaaa cgctgggaac
      961 ccattataat attatcggtg ttaccatata ttcttacgaa gaaacatcgc ttaattaccg
     1021 cattccaagc gacattgttg aatccacttc tattgagaca aacctgagca acagtacact
     1081 gatattcccc aatcctgcaa acaacctaat cacgatttcg gcaaacgaaa aactgaccaa
     1141 tctgaatatt gtcagcgctg aagggaaaac cgttatgaca atatcgcccg actccagaga
     1201 cgttaccatt gacatcagtc aattatcatc cggattctat tttattcatc tcgaagggca
     1261 gtaccaaaaa ggagtgaaaa agctaatcgt tgaataacta aaataaccca ctgcaaaatc
     1321 ccgcctgaaa acgggatttt gttttttcgg gaaattgttt tataattgca caaccttaaa
     1381 ttgaaaatta atattcaatg aagaaaaaat acatgcttat ctttttttcg gttgtattgg
     1441 ttttctctac ctcctgtaca aaatttgaag aaggtgcttt cataagccta cgttccgccg
     1501 aatttaatat aatggggacc tggaaaataa gcgaggttct ggaaaatgac cttgttaacg
     1561 actctctttt aaatgaagaa gctaacaatc tacttgtttt tgccgactct tcgtctttca
     1621 cttttcaggt attgcggaat gggaatattg cccagaactt agcgggaacg tggcagtata
     1681 attcagataa tcagcactta agctttaaaa ctgatgctga ttcaacattt caggtttacc
     1741 gtataattcg gcttaccaac aaagagatgt ggctggttga tgaatgtcag gaccaaacac
     1801 gtagtgggtc aagtattgaa aagagatatc agaaacaata actaaaacta agctaatatg
     1861 agaaaaattg caagtattct tttaattgct gccgcagcag tatttgtgtt caactcctgt
     1921 caaccttatg aagaaggccc ttcaatcagt ttgctgtcga aaacaacccg gcttgtcgga
     1981 gactggacaa ttgacacgtg gctggctaat gacacagaac aggatatcag ctcaacacaa
     2041 cccagaatga gcttcgttaa agatggctca ggcagtatga caataaacgt gttcaatttt
     2101 gccgtaacag ttgatttgga atgggaattt gtaggagacg atgggatcag gtatcgcgtt
     2161 aaaaaccaga acgatgcatg ggaagactgg acagaataca ccattctcag actttcgaac
     2221 gatgaattat ttattgagag gacctatctc cccgaaggct cagaagcgaa tgtaacagaa
     2281 agaattgagc tttcaaaaaa ctgataaatc attaacatga tataaaaaac ctgtgacaaa
     2341 tccacaggtt tttttatttt tactccagta aaaccgacaa tcaaatgatg acaaaagacg
     2401 attttaaaaa gcagatactg gaaggtattc ctaaaacttt accttctatg aaacatccca
     2461 acccgcaact caatcatgca cccaaaagga aagaaatact gagtgaaatc gaaaagaagc
     2521 tggctataag aaatgcctta cgttatttta gaaaagaatg gcacccagtt cttgcacagg
     2581 agtttaagga agaactcgaa aaatacggaa gaatatacat gtaccgtttt ttgcccgatt
     2641 acgaaatatt tgcacgtgat atcagctggt accctcacaa atcggaacag gctgcagcca
     2701 taatgctaat gataagtaat aaccttgata acgcagttgc acagcacccc caggaactga
     2761 taacctatgg tgggaatgga gccgtttttc agaactgggc acagtacctc ttgactatgc
     2821 aatatctcgc ctgtatgact gatgaacaaa cattggtcat gtattcgggg catccaatgg
     2881 gccttttccc ttcgcacagg gaagccccgc gcgtagttgt taccaacggg atgatgatac
     2941 ccaattattc gaaacccgat gactgggaaa aattcaatgc tctgggagtt acacagtacg
     3001 ggcaaatgac cgcaggttcg tatatgtaca ttggcccgca gggaattgtt cacggaacca
     3061 ccattacggt aatgaatgct gtacgcaaaa tcaacaaaac aaacacgtca agtgaaggcg
     3121 tactgttcgt ttcttcgggt cttggaggga tgagcggcgc tcagcctaaa gccggtaata
     3181 ttgccggagt ggtgagcatt gttgccgaaa ttaatccaaa agcagccggt aaacgtcatg
     3241 ctcaaggttg ggtagatgaa attacatccg atatcgacca tgcaattgac agggcattgg
     3301 aatataaaaa aaccaacagg gttcggtcga ttgcatatct cggaaatatt gtcgatttat
     3361 gggaaagatt cgccgaacgc aacattccgg ttgatcttgg ctcagaccag acatcactgc
     3421 acaatccatg ggcaggtggc tattaccccg ccgggttaag tttcgaggag tcaaatatta
     3481 tgatgtcgga aaacccggaa aagtttaagg agcatgtgca gaaaagtctt gtaaggcaaa
     3541 ttcgtgcaat aaacaagctc tccgaaaaag gcatgtactt cttcgattac ggaaacgcat
     3601 ttctgttgga aagctcacgc gcaggtgctg aggttatggc tgctgatgga atatcgttcc
     3661 gctatccgtc atatgttcag gacattatgg gacccatgtg ttttgactac gggttcgggc
     3721 ctttccgatg ggtatgctgc tcatcgagac ccgaagacct cgacctgaca gactcactgg
     3781 ctaaggaagt tctattgggt ttatcgcaaa aagcaccaat agaaatctca cagcagatgc
     3841 acgataacat caaatggatt accgaggcca agaagaacga tttggttgta ggttcacagg
     3901 cacgaatact ttatgccgat gcaatcggac gtatcgaaat tgcaaaagca ttcaacaaag
     3961 ccattcgcaa tggaacaatc tctgctccgg tagtacttgg gcgcgaccat catgacgttt
     4021 caggaaccga ttcgcccttc agagaaactt caaacattta cgatgggagc cgcttcacag
     4081 ccgatatggc gattcaaaat gttatcgggg attcatttcg tggtgccacc tgggtatcga
     4141 tacacaacgg cggtggtgta ggctggggag aagtaataaa cggcgggttc ggaatggtgc
     4201 ttgatggtag tgctgaagcc gaccgcagac tcgaaatgat gctgtactgg gatgtaaaca
     4261 acggtatcag caggcgttgc tgggcacgca acgaaggagc tattttcgct gctgaaagag
     4321 caatgaagac aaataaaaac ctaagcatca ctttgcccta tattgccgat gacagcataa
     4381 tagattcact gtttaaccaa aataatcgaa aatgaataaa cctattgtaa tttctctgat
     4441 agcaattgtt atgattacgg tttttgtaaa caaacaatcc gttgctgcag taagcagcaa
     4501 gtatatttct gacatgaccg ttgcaaaggt catcaacgaa ctgaaagcca aatatccgga
     4561 acaggatttc cgtattgaac gcggggttaa tcaggttgcc gcgctttggt ttcagaccga
     4621 tggatcggaa acggatttca ctgaattttg tctggccaat tatattggta acagcgatga
     4681 actcgatgtt gttttcagca aactggagcg caacctcgaa gtgctatacg gctcgttcaa
     4741 tcatattgta atcgggctga tggagccctt gcatctcgac atgggaagca tccatccgat
     4801 tgatgaaatg ttcggtgcct attcaccggg ggcacatctt tccgatgact ttttcagcaa
     4861 taaaatcgct tttattgtaa tgctgaactt ccctgcttat actttaaccg agaaaaaagc
     4921 aatggggaca aactggactc ccaaacagtg ggcgcacgcc cgcatgggtg acatgttcac
     4981 acagcggatt ccggcagaac tgatattaaa tgtttcaaca gcaacaacca acgccgacac
     5041 atacatttcg gaatataata tcatgatggg aaaccttcgt aacaataaaa acgaaagcct
     5101 ttttccagtc gatatgaaac tgatatcgca ttggggactg cgcgatgagc ttaaatcgaa
     5161 ttacaataat accaacgggt tcgaaaaaca aatgatcatt tacaatgtga tgctccatat
     5221 tatcaaccaa tcgatcccgg ctatggttat caacaatgcc gatgtaatct ggaatccgat
     5281 ttcgaatacc gtatggaaaa acaatcaaga aattgcagtg cagcgcgaac cggatacaag
     5341 ataccagcat ttgcttcggg ttttcgaatc gatgaaagct attgatgagt ttacccctat
     5401 gtatccgact tacatcgaaa gagcgtttga tggtgggatg gaattatcac tcgaagaaac
     5461 cgaagcatta tttgtcagtt atatttcatc gccggtgatt aaagacgttg ccaaactgat
     5521 acagaaaagg ctcgggcgga aactgcaacc gttcgacatt tggtacgacg gcttcaaagc
     5581 acgcagcagt attaacgagg atgaattatc ggcaataacg cgcaaacgtt atcccaacag
     5641 ggaagctttc gcgaaagaat tacccctcat tttggttaag ttgggttggg ataaaacaac
     5701 tgcatcgtgg attagttcca aaatttcggt tgacccgtcg cggggtgcag ggcatgcctg
     5761 gggtgcagaa agcaaagaag atgttgcgca cttgcgtaca cgtataggtg caaatggcat
     5821 ggactacaaa ggatacaaca ttgctataca cgaatttggt cattgcgttg agcaaaccat
     5881 taccctgcat aaagtaccat actatatgct tcatggcgtt ccaaacacag cctttaccga
     5941 agccgttgca tttatgtttc aagctaaaga cctgcaatta cttggcgttg aaaacacagc
     6001 agatgcaaag gaaattgagg ctttaacagc cctcgacaat tgctggtcgg tttacgaaat
     6061 tatgggggtc agccttgttg accaacgcgt ttggaaatgg atgtatcagc accctcaagc
     6121 tacacccgaa gagcttcgca ttgccgttac tacaattgct atagacgttt ggaatcaata
     6181 ctatgcaccg gttttcggtg taaaagattc tccaattctg gcgatttatt ctcatatgat
     6241 cgatgcccca ctctacctat cgaattaccc agtaggtcat atcatcgaat tccagattga
     6301 agattatgtt gaaggaaaag attttgcttc cgaaatcacc agaatgttta cccagggcag
     6361 aataattcct caggtatgga tgaagaacgc cgtaggcagc gaaattgatg caaaaccctg
     6421 cctcgatgat gcagcgaaag cagtagattt tttgaaaaag aaaaaataaa taaacctcac
     6481 attttcgtat ttaagagcga aacattctcg ataaaaaaaa ataatataaa atcgctttaa
     6541 tcaaattcag ggttttatct taactccttt aaatattttt gtaaccatct gtaatacaat
     6601 ttattatagt atttaagcaa caaaaaggaa tgagttgaga acaaccatca aaaaaatgac
     6661 tatatttgtt tgagctgaat aaaaagtgtt aacaagttta gtaaaatgtt acaactcgac
     6721 agatataaaa ataaatttac agggaagaca attcttattg ctgacgatgt tgaggatgtc
     6781 ttgttttact ataaagcact gcttcagttc acaggtattc gtattatgga agcccgcagc
     6841 ggtaacgaag tgatttctat ttgccgtcac aaacccgata ttgatttaat aatgcttgat
     6901 attcagatgc caaacggaga cggaatgtac acgctggagc ggctgaaaga gatgcattca
     6961 cgggcagcag ttgttgctca gactgcacat gccttaccaa ccgataagat taaatacatg
     7021 aagcttggtt ttgatgatta catctgtaag cctctgcgtt cagagacact ctttcagatt
     7081 cttgaggacc atctcactgt aacggaaagc gaacggaaat aaattattcc agagatttat
     7141 tacttttgtt attaattaca accgcaattc agggtggacg acaaacgtat tttcagcctt
     7201 attaaacaag ggaatgagga tgctttcaac aaattgttca gggcatatta ttcaaaacta
     7261 tgccattatg ctgtaatgct gggttgctgc ggcgaagatg ctgaagaaat tgtacaggag
     7321 ttgttcacaa agctctggat gaaccggcaa aaaaccgaaa ttgatatttc agcaaaatcg
     7381 tatttgtaca aatcgacccg caatgctgta attaatctcg ttgaacatca acggataaaa
     7441 caaaaatatg ctggtgaaga aatacttgcg aaaaccgagt ttttcgatgt aaacccattg
     7501 ctagttgccg aactccagga gcacatccaa agcgccatca acaaactgag tgagggtcgt
     7561 aagcaaatat tcattctaag tcgcagcgat gggttgagct accaagaaat tgccgaaaaa
     7621 ctgaacatct cacccaaaac ggttgagaat cagatggggc acgcactaaa acaactcaga
     7681 gaagaattaa aagattattt acccatgatt ttagtatctg gtttggcata ttttttttgt
     7741 gaatgggggt atttatgtac ttaactgtca tatagaatat gcctgaaatc aacacatacg
     7801 attacgagct tctggcagca tttattgttg gcacactcac cctggagcaa caaaaagcgg
     7861 tgcaggaacg tatttcatca gagcctgatt ttgataaatt ataccgccaa ttgctgttca
     7921 tttactcagc gtatccggct ataaatactt tcgataccga aaaagcacta cgaaaagttc
     7981 acgaaatagc cggaaagaaa aaatcaaaaa ttcatattac accaagaaca cttttttatt
     8041 cggcagctgc agcagttatc attcttattg cactaaccct tgcaaatagt ctctttctta
     8101 ctggcaataa gcaaatcaat tatatcttag tcgcttcaac cgacagcgta catgtcacca
     8161 gtctttcgga tggcagcaca gctacattga ataaaaattc aatccttcgt tatgagtcag
     8221 gttttgaaaa ctcggaattg cgcagggttg aactcgaagg agaagcattt ttcgaagtta
     8281 aacacgatgc aaccaagcca tttgtggttg ttactcaggc agcggaagta cttgttaccg
     8341 gaacagcatt ctcggtaaac gatttctcgg gaaaagaggt tgtggtatgg gtagagcagg
     8401 gttctgtgag cgttacttct gctacaagca atcaaaccat tcacctgaat ccaggagaaa
     8461 aagccatttg taacaaaact accggcagta tttcgaaaac ttatggctca ggagatgaaa
     8521 cattctggaa aagccgtttc ctctcgttca cttctacccc actatcagat gcctgcatca
     8581 cattagaaaa aaattatggc tgtagcatcg aaataacggg cagcaaactg catgaactaa
     8641 ccataaccgc atcattcgaa aatgaagaac cggagcacat actcgaggtt attgccatca
     8701 ccctgggttt acaactcgaa gcatcaaaca atcattatcg tctgtatgcc gaatagattt
     8761 accgcaagga tccaacttct tatcctgata ttactaactc cggcatttca atcgttttct
     8821 caaaatgcat atctgagcaa aaaaatagat atcgatctga aaaacgtaag tcaggcaaaa
     8881 gcactcagtg aaattgagat caggggtgga tgcaggtttt cttatgggca gaatgtaatc
     8941 aaagccgatt ctatggtaag cttgtatgcc aggcagcaaa gcattcagca tattcttatc
     9001 acgttactgg gaccatcaat agaaatgatt gaatcgggaa atcacataat tcttcgacga
     9061 aaaaacgagc ccaagcctct gtttaccggg gttgtggtaa atgaaaaaac aaagtatgca
     9121 gttgcgaatg caaaaatttc ggagaacaac cgaagtaaaa cccgtaccga ccgaaatgga
     9181 aaatttagtg taatgctgag cgaatatccc tgctttatca gggtttctgc caacggatac
     9241 agggacacgg tatttcaggt gacggaaacg tataaaaacg tatcagtaag tttaatccct
     9301 gattccaaag ttcctgaggt gcttttcaaa gcaccatcac tggcacctga gcaactcgga
     9361 acgggcagtg gcagttccgg tgacatgaaa agtgcattcg aaattcgccc tttgcacgat
     9421 agtatgactt accagtcgct tgagaaaaaa gactttcaga tttcggtact tccatttgtc
     9481 ggcagcgatt ttgcagcatc ccgtgattat aaatacggca catcgcttaa tctaccctgc
     9541 ggatacaccg gaggagtttc gggggtagaa atcggattac tcatgaacat cgtgaggcag
     9601 aatgttatcg gctttcagtt cggaggtctg ttgaacgttg taaacggtag tgtaagcggt
     9661 acccagatca gtatcgggtc aaatctggta ttcaacaatt tttcgggaat tcaggcagca
     9721 ggagtgtata attactgccg taatgaattc aatggtgtgc aattttcgct tatacataat
     9781 aggctttccg gagcttcggg cggtgtgcag ttttcgctga tcagcaattt aacggaagcc
     9841 gatttttccg gtacacaagt atcaggggtc tgcacaataa accgcgggaa ttacgtggct
     9901 agtcaggtag ccttaggggc aaatgttaca gatggaacct gcggggtgct tcagttatcg
     9961 gtattttcga atacagctga tactgttgaa ggggtgcagt tggctttggt gaatagatgt
    10021 aaaaaatgtc acggtgttca ggttgggctc attaatgtgt cggaagataa cgacggaatt
    10081 gccatcggtg tacttagtgt agtaaaaaat gggtacagag cagtggaatt gagctacagc
    10141 gaattgaata acgtaaattt ttgttttaaa agcgggttga gaaaatttta taatattata
    10201 aacacaggca tcagcccaca atccggtaac tggcgcatga atctcggtta cggcatcggg
    10261 tcttctgttg aattcggaaa gcggtggatg ggaaattgcg atctggtgat aaaccatgtt
    10321 tccgaaaaaa acagctttgt taaggaccta aatctgtggt tgctcctcaa accacaaatc
    10381 ggtgtaaata ttgccaatac atgcgtgctg tttaccggag catcgctgaa cctttcaata
    10441 agcagattcc gcaatcctta cactaacgaa tttctcactg agctatcgcc tgaatattcc
    10501 atatttagta aagagcataa caatacgaag tattcgatgt gggtaggtta tagcattggc
    10561 atacgatttt aatttttttt taatttaata ataggggtaa ttgtcgacaa agctgtcata
    10621 gtgatgtaaa taataaaaat aacatgagaa aaataatttt tacattcgga tttctcgcag
    10681 ccattactgc tgcatatgca caaaacgaca gcctgaacag aagcaggaat aattcagaaa
    10741 taaaaaccct tttcgactct gatggtatta cttcgcacgg gatttatgcc ggctgcagca
    10801 tgtactacac ccagattgca ggacgaaacg cgtttgttgg cggaataaaa ggtgcatggg
    10861 tgattgacca tgtttttgaa gtgggtggag agctcaatgg ttttttatcg gagccattac
    10921 ccgatgtaac cctcagtaat tcatactacc tctattccgg agcttacggt ggtttacttt
    10981 tcgcgaccaa tctgtttcca cagaaagcca ttaatttggc gataccaatt agcatcggtg
    11041 ccggcggtgt agcttatgtc ggcaattatt actacttttc ggaagactac gagtactatc
    11101 ccgaaagtta ttatgcctat ttctatgttc aacccggact ggaactgcaa tttaatatga
    11161 cacagcattt caggcttgca atacatggca gttatcgcta cacatcgaac atttatctcg
    11221 aatattcggg cggtaccgga cagataattg gcaacaacaa cctgatgaac ggattcaatg
    11281 caggtattac attaaaaatt ggtaaatttt aaaaaaacat aagaacatga aacgtatagt
    11341 cctaatttca gttactgtag ctctactggc cggaatgacg gcatgttatt tcccgcatga
    11401 ttacatccgc ggtgtcgggc aacttacaag catcgaaaaa gatacccgag gagttaatgg
    11461 aatcgagata aacgatatta tcgatgtggt tctcgtaaaa agcgatactg ccaagattat
    11521 tattgaagct caggaaaata ttatcgattt ggtaagtacc gaagtcgata acggaatctg
    11581 caaggttgag tttgtgagag ggtacaatgt gagaccaaca ctaccggtaa ccgtctatat
    11641 ttatacaaat attatcagca gcatcgaaat tgatggttct ggcgacttga caacaaacgg
    11701 tattttcgac actcttggca atcttgaaat agacattaat ggttcgggta atgttgattt
    11761 tgcatggctc gaagccaacg acatctatct cgaaattgac ggttcaggtg atataaatgt
    11821 caccgggaat ggacaaaata tgagtaccac tatcgatggc agcggaaacc tgcacatggg
    11881 taccgaactg gccggcacat cgtttcgcat caacggaagc ggcgatatat tcatcagtgg
    11941 ctggtcggct tcgaatattc tgcatattga tggttccggc gatttctacg gtttcgacct
    12001 tgaaaccgaa acaacaagcg tatttatcga tggcagcggt aatgccgagg tgtgggcaac
    12061 cgaaaatctg ctggttgaaa tcgatggcag cggggatgtg gtttaccgtg gcaatcccaa
    12121 tattaattca ggtggtagcg gaagcggaaa cctgataaac gggaattaaa acgtaaaccc
    12181 gtcattgcaa ggggtggaaa cccaaagcaa tctgcatcta cgtgcagtga attaaccgcc
    12241 tccgtgggtg tagattgctt cgttgcacta ccaatgatat tgcaaagtga ctcaacaagc
    12301 agtaagcgtc cacgcttgct gcatttatca aattctattc cagcgcattg ttcagaaaat
    12361 cattaaaggg atgttgtgcc ctgaacacct taaggatttc atccgtgatg ttttcagaaa
    12421 acatctcatg atcggaaaat gaatgaatca cagtataatc tttgtaaacc agtaaagagg
    12481 catccggaaa gtctttcggg aagtctttcg ggcaagtctt aagtttatcg ccataaatcc
    12541 cgctgaaata tgtagtaaac tcctttgctc ccataattct tttgaattcc tccgtattgt
    12601 aataaatttc actgcgaatg gctttcagta tctcaggcgc aggcatataa atacccccgc
    12661 ctgcaaacga tgctcccggc tgcagatgca cataatatcc tgctttaatg ctttttttac
    12721 cgccttcagc aatatatgct ccgaagttcg ttttatacgg ctctttgttg tgcgagaacc
    12781 tcacgtcgcg ataaatacga aaaatacaat ccttcgcttc gggatgccct atcgatggat
    12841 cgaattcgtt aataccgatt accagttgct gcacaaactt ttcgaacagt gaaaccgact
    12901 gcctgtatgc tgcttcattc tgcctgaacc actcgcggtt gttgttttgc tgaagctcat
    12961 tcagaaaacc gaaaatgcgt tgtaaattca tttttcttat cgattaatgc catgctaaat
    13021 taactaaaac cacaggcaaa tgtttacata gtaccttatt atttattccg atattctgtt
    13081 tttctattga taaaatgcaa tagtctgcta actttgtatg atacaaaacc attcaatatg
    13141 caaatcaaat tcatcggtgc agcacaggag gttacaggca gcaaacacct gattaccaca
    13201 aaaagtggta aaaaaatatt actcgattgc gggatgtttc agggaaaagg tctcgacacc
    13261 gaccggatga accgagaact gggattcgat ccgaaagaga tcgatcatgt gatactgagt
    13321 catgcccaca tcgaccattc cgggctgatt ccgtatattt accggctggg cttcagcggt
    13381 tcggtggttt gcaccagtgc cacgcggaac ttatgtgcat ttatgctgcc cgattcgggt
    13441 ttcattcagg aacacgatat gaaatggttc aacaagaaac gggcaaaaca gggtctgccg
    13501 ccattggaag cgatatataa aaaagacgat gccgtgcagt gtatgaaact gtttatcgcc
    13561 gttgattacg accgaaagtt ccgtatcgat gaaaacatta cggtgcggtt caccaacagc
    13621 ggtcacatgc ttggcagcgc agtagtgaat ctcgagataa aagaaaacgg taaaataacc
    13681 cgcattgcct ataccggcga tatagggcgc ccttataacc gtatagtaaa gccccctgcc
    13741 ccattccctc aggcagacat actcattacc gaagccactt acggcgatcg tacccacaac
    13801 acccagaacg agtcggaaga aaccctgcta aacattgtga ttgacacctg cgtgcgtaaa
    13861 ggcggtaagc tgattattcc ttctttcagc gtgggacgta cccaggaaat tgtgtactcc
    13921 cttaataact tttacaacga aggcaaattg ccggccatcg acgtgtatgt cgacagcccc
    13981 ttgtcggtaa atataaccga ggttttccgc attcatccgg aatgtttcaa cgacgacgta
    14041 ctcgaggtaa tggaaactga tgataaccca ttcggctttc gtaatctgaa atatataaaa
    14101 aacgtagcag agtcgaaaaa actgaatacc accgaaaaac cctgtgtcat catctcagca
    14161 tcgggtatga tggaagccgg gcgtgtgaag caccatctgg ccaacagtat cagcaatcca
    14221 cgcaacaccg ttctggccgt aggatattgt gcacccgaaa cgctgggggc taaaattctt
    14281 cgtggcgaca aagtggtttc gatatttggt accgaatacc cggtacttgc tgatgtgtac
    14341 cggctggagt cgttcagcgg ccacggcgac tacaaagaaa tggcgcaatt tataaaatgc
    14401 cagcaacccg aaaaacttca gcagataatt ctggtgcatg gcgaatacga tgctcagcaa
    14461 cattacagcg aatatctgca cagcatggga tataagaata tactcatacc tcaaaaaggc
    14521 gttgaaatta ccgtgtagaa gcagatgaaa attagccttg ttgttgttat actttcgatg
    14581 ttgtttttgg tttcatgcaa aaacaacgaa atcccttcga tgaacatcgt aaaagaaggc
    14641 gatgtagggt ggaattcgaa agaagcatgc acaattgagt attgcaccgg tggcgatacc
    14701 ctgatactga aagcagcaat caagtgccgt ggcggagtgt cgagtaaata tttcaaacac
    14761 tcctattcgc ttgaactgga tgataaattt agtgtggccg gattgccgcg cgacgacgac
    14821 tggatattaa atgccaacta catcgacaaa acgtttatgc ggcataaaat aagctatgac
    14881 cttttcaggg aaatgtcgcc aaacaatgct gcctcgcagt gtgcgtatgt gaacctgctg
    14941 ctcaacggca aaccaatggg gctgtatgta ctgatgcagg aaataaatgc ggggatgctt
    15001 gacctcgaca ggaaagaccc tatggcaatg ctgttcaaag atccgcctgt tttttatgcc
    15061 gacaagatta cttcagtgca ggatagcctg aactactatc agcagaaata tcccaaaata
    15121 caacaatgcg ataaaacatc aaccatcgag gagtttaaaa actttttatt cttcagcagc
    15181 gactcggcat ttgctcagaa tgtttcgaag tgggttgaca ttgacaatgt gatcgactgg
    15241 catattgtac tattgttttc gaataacagc gatggtatta tgaagaattt ttacctgtat
    15301 aaacccgact cacacactcc atttcgtttt gccatctggg attacgacca ttcgtacgga
    15361 agagacggag ataacgagcc gcatcatgcg aatcaggaac tgaactggca gcgttgcatc
    15421 ctgctgaaac gcctgtacca gattcccgcg acaggctata cccaaatgct taaagaccgc
    15481 tgggcatacc tgcgaaagca cgacatcatc agtgtggcac acttcaaaga gctgatggat
    15541 gaaaacgaca aggctatcag tcaggcggtt ataaacaact ttgcactatg gcccgtaaac
    15601 gaaaaatggt acttcgatgc caatacctac caacaggaaa ccgctttaat gctgaaattt
    15661 gttgaagaaa gaatccccat gctcgatgcc tatatcgaaa agctttaaac ttaaccggtc
    15721 agaacgcact atcaaaataa ccgggggtta atacaggcct gattatcata gtttcctgat
    15781 tacaaatttg ctggaaaatt ttaaattttc attctgaaga ataatgatgt acaatccatc
    15841 ggtataatta cgcaagtcaa acgaacattt atccaccaat tcgatatttg tctttagaac
    15901 tatttgaccg gttgaattaa taattgacaa atggaatgta ttaaagtctt tgttattatt
    15961 cataatatca atgaaatcgc tagcagggtt tgggaagata atgaaagtat ttgtcgttga
    16021 agcttggttc ggaatgccac tgcccaattt cttatcgatc gcatatgatt ttatcctgta
    16081 tgggtctggt aaatcaagga aaaaccaacc atacaatgtg tcagaagcat atatcttcct
    16141 gaatccgata tacgtgtcgg gaagtgtaca agttccgtgc gatgtacttg atggacccca
    16201 gggtggagga atatgggagt cgaaatagta ataaaaacac gcaccatcgt agttatcgtt
    16261 ccaatgtaat gcattatcaa tctctgtccc gagcaataat ggttctgcat cggaagaacc
    16321 gggttgggta acaaattgca cagaatttaa tgattgtaca gaaaatgttt ctgaactata
    16381 agatggcgaa gagctgtgct ctctatgaaa acgaatatca ttaatattgt cagaatcaat
    16441 gtcgatatca aaattaaaaa agccttttat aactattggc aaaatcgtat cagggatatt
    16501 tacataagta atttccggat tcaatgtatc acccgcaata aacatctgtg catttatatt
    16561 gcaataacca aaaacaagga taatgctaac ccaaaatgtt tttagcgtag atgatggcct
    16621 attcataacg gttacagagc ttaatctcac attacactat tgttccaact caaccaatcc
    16681 atcggtatgg tttatttcag tttttttgat atacccctcc ttttcgtatt ccatataaaa
    16741 atcccattgt cgcaagctca gaccataacc aatcatctca tccctgataa aatttccggc
    16801 agtgtcggta tagccttgta agttataggc acttttttct ttgtctccga cctttactat
    16861 aactttaaca agtacactgt cgatgggttg ctgtgttatt ttatcaacca cagtaacatt
    16921 gaaataaatc ggaccttcgc aagagaccgt gaaaattccg ataacgataa gtaccaccgc
    16981 cagtttaatt gtattcattg tagttttagg tatttgccta agcttgctca taaggttttc
    17041 ttcgtttttt caaattcgac cacaattttt tttgcctttt cataattatc gtctgcaaca
    17101 attactcgta cgccccccac ccgacctcca tggtttgccg acttcattgc ataaaaatat
    17161 aaaagaagca tcagctttca aaagaaagcg ggattttttt ggtggaatgc aacatctgct
    17221 acccgtcgtc aacggaatgc agctactcgc tttttacaaa tctttctgta aacacgcgat
    17281 ttgcagtttt tattttaagc agataggtgc cggaatccca attgtccata ttcaacacac
    17341 agttataacc cgcggggttg atcgtcatca gccggacacc agctatgctg tatacctcaa
    17401 ccacagctat ctgtgcctca ttgctagttt ctacatgcag caggtctgtt actgggtttg
    17461 gatagatacg tatctctttt agtgtttgac tgatattttc gatgtcttcc gtattttcgt
    17521 tgctaataag tttcgataaa ccgccgccaa aggttgtgat ccatttattg ttcgatgcat
    17581 caaaagctat atcggtaacg ctattcaaag gcaaacctga attggctgca ttataaacaa
    17641 cccattcgtt cgtgttataa ttaaacttag ctataccgtg ttcgtaggtg ccagcccaaa
    17701 tattcccctt acggtcaatg ccgaggcatg taatggtgtt ttcgggcaaa ccggaattgg
    17761 aagtattgta gtttgtccag gaagttccat caaatcgtga aagtccttca ccggcaaccc
    17821 agataacatt gttcgcatca attgcaatat aatcggtgta gttgcccgga atatttgaat
    17881 tactctcatt gtattgtgtc cactgttgcc cgtcgaattt taccagacca ttatgctggc
    17941 ttgtcatcca gatagtattg ttattgtcaa cagcaacatc cattacccag ttgcccggga
    18001 tttcggagtt ttctgtatta tacgaaaccc actggttgcc gttgaacatt tgcaaaccgc
    18061 catgcaatga accacaccac aggttgtttt gagagtcaaa tgcaaacgac ctgatattgt
    18121 tatcgaagag accggaattc gactcgttat aataattcgt attgttctga acgaatgaat
    18181 aggctccagt agctgctgcc cacaggttgc cgttattgtc aaccgccagt gcattgatcc
    18241 actcacccag atcatataaa tatgtgcatt gatcgctgta aaaaccattt atttctccgt
    18301 cccttgctcc tgtccacaca acgcctgcat tgtcgacaca tacgcggtac tgataatttg
    18361 atgcaatgct tgattcagaa ataggtacag ggttccaata cggatcgtta tagattttaa
    18421 gtccctgaag actggaacct acccatattt tctcatccga agtttgacaa atatcatata
    18481 atgcacctgc aggaatacct gaattacatg tggtacagta gatccaggta tcatcgttta
    18541 tgatatacaa gccataatcg gatcctatcc aaacatcacc gttttttaaa atctctatgc
    18601 ttcgtatggt attttggaca agtatactat tgtcctcatt gtaattaacc catgaagtgc
    18661 cgtcgaatac agctattcca ttatccaaag taccaaccca catcgtttca gcctcaaagg
    18721 ctattgtcaa agagttgttt attggagcac tattgttgtc attgaaaact gtaaacgtaa
    18781 caccatcaaa tgttgcaata cctcctccca aggtagcaat cccaagctgt cccccgttat
    18841 ctgatgttat atactgaaca gagttatcag gcaaatctga attggcttga ttataaataa
    18901 tccatgtacc attattcaga taggcaattc cgccatagtt tgttccaatc caaacaccat
    18961 tattgttatc gcaaaatatg gtattaatgc tattgtccgg caatggggag ttgtccgtag
    19021 tgaatgtctc ccaattcact ccgtcgtacc tggcaacccc accgtaacag gttccgatcc
    19081 atttgttcga ttggttatcg acagctattg atttcacaat attatcggga agcggactat
    19141 tcgatttatt atagtgcact tttgcatggg tagtctgatc gacccgcacc agtccagtac
    19201 ttgtccctat ccaacagtag ttgccatcct cagcgatagc atacactacg tctgctcttg
    19261 agaatgtctg ccattggtcg ttttgtgaaa aaatctgcag catgaaaaag aactgcagca
    19321 atgttaatag tgctattgac ctcattttag attgattgtt acatttaata taaaagaaaa
    19381 cacaatgtaa taatttttat tgtacgattt accaaaaaag taaaacattt ttgatatggg
    19441 atattacagg tgacgcatta gcttttggga caaatgttta ccggaaatac tccctcgtcg
    19501 caagcatggc gaggatgggg gcaattttaa ttatcaaggc tttatcaaat ctatgttagg
    19561 actactgtta tcgcaaaaaa aactgcacat atccattatt gttatttatg cggcatggaa
    19621 aatgcttctg attactgcaa taaacctatt ctattattcg tttacatttt atcaacttgt
    19681 ttgtttgcca tagcttctat ggaattcgca attcttgaca cattcaacat caggcttacc
    19741 aatacagcac ttaccacaaa aatgcaaaca attcctagta atatgccggc agcctgcttc
    19801 attgcgttgc taaatagatc caaattgaat gcaacatcaa aaacgaaaac aatgcctaca
    19861 agtgttaaaa agaacagtag tcctactccc gttttcgtta ctaaattttc cagttttttc
    19921 gtgttcatgt ctcttatttt tattcatatc tgtttaacat ttcaaattga gtctgctttc
    19981 cttagctttc agaacaaacc gttttaaacg tgggttccgg tctccgcttg tacattagct
    20041 gagcaatcgc taagcttcga taaacgccat taaaaaacaa cattttatct ttcaagcatt
    20101 cgttcgattt gttgcaataa aactttcctg caagatatcg ttatccaccc caattacaaa
    20161 caaaagatac gccttatttc tctaccaaat atataaaaat ttacttgaat aaaggctttg
    20221 tccggtaaat catacacaat tattctacat aaaaacaagc aaagaagctt aattgtctat
    20281 ttaaattaga ggcgtcaact ttcgaaagaa agcgtgattt tttcggcact accaaataag
    20341 aataccggaa ttatcgataa aatggggtaa tagtcaaaat aaatcgcatt gcaaatgcat
    20401 acccaaaata cgccctcgct aacgagggca aagatagtgg tgttaattta cgctttctcc
    20461 gttttcctgt tattgtaata gttaatattt cagaatcttg ctatttgcaa caattctttc
    20521 tgcggaaatg atttgtacac tgtacatccc attcggtaag tggctaatgt ttatttcggt
    20581 tttgccgttt gcgttttgag ataggacttc cttgccaagc atgtcgtaga gtttcacagt
    20641 agcaaccccg gtggggtcaa tcataaagta gtcgtgggtg gggttgggaa acagtagcat
    20701 atccgacaaa ctatggctat tgatattaac accggagtta acggtaattt ctgcaataac
    20761 ctgtgtcgaa aaatcgggat atgcaattat cgcgtcattg gtcatgacaa ccgtataatc
    20821 gcctgctgcg ttaaaaataa gcttaccttc aaccacggta tggtagccat cgggatttac
    20881 cgaataattt gtaaaaatat cgctaaatac cgattgatcc gcaaagagct catctcctgt
    20941 atctgcggtc gtagatatgt actgtgtccc cagccatttg ttgttcgcat catcaatcaa
    21001 ctccgatatt tcgaacaaat ccgataactg aagtttgttt tcgttacaat atacgcgcag
    21061 cgaagaagaa catccattaa tattcaaagc actcaactca tttccggtac aacttacctc
    21121 ggttaatgag gtatagtcag ttaaatctag ttcggttaat acattgttgc cgcaacttaa
    21181 aatatgtaaa cttgtacatc cgcttgtgtt taaagtatgt atctgattat tgttgcatat
    21241 aaatgtttgc aaattctggc tattgctcac atctaaagtt tgcaacagat tgaagttgca
    21301 acaaaattcc tgcaaattcg gacaagagct gatatcaata gttgaaagct ggttgtaatc
    21361 gcatgataat gcttgcaaat tagggcaact attcaggttt agctctgtta actggttgtg
    21421 agagcacatc agcattgtta attcagtgta agtgcttaaa tccagatccg atagctgatt
    21481 aacatggcag taaaaccttt gtactgctgg gctgcatgtt acacttgtta acaggttctc
    21541 ggagcaatat acaagccata aggatgagca ggtgctcacg tctaatgttg tcaattggtt
    21601 atagtaacaa tataattgct ctaactcatt acaatcagtt atttgcaagc tgcttatctg
    21661 attgttgaca attgtactgg tttctgaatt gtaaaaaccg gagcaatcaa aatagtaaaa
    21721 ccggcaattc tctccggctg aaatggttac ggtatattcg ccttcttcgg catagttatg
    21781 ggttaattgc acattggtat atgcaccgcc atcatatccc aatccggttt cagtatcgat
    21841 tgcactgcca tcgccccagt ttacataaaa ctcttccgca ttggtagcct gagttttgta
    21901 gatgattttt tctccagttg catgccaggt aaatgtaatc tggtcctgtg cagcaacttc
    21961 aacacaacgt aatgccactg ctactaacag cattattcct gatttgcaca atttgctcgt
    22021 tctcattcgg ttaagtttta attgttatat cgcaagtact tctcggttca acaaattctt
    22081 aaaatagttc taccacgggt tcactatgtc cgggtcaatt ttttaccgct catataaatg
    22141 tgaatattac caataaaccg gtcacctctc ttcaatacaa aaaacatgta gaaaatcctc
    22201 tcatttatgg tttttatcaa atatcaaagc tttgagtaca aaacaacgct tacaagtgta
    22261 aaatcagatg cagtggaagt tttcaatcgc taaattatat aatacaacat agcttgcaaa
    22321 gaaaaaagca tgatttttgt tatattttaa aaataaatta gaacaagatt ctattactta
    22381 taaatcaata ctttagcagt tgtaatatgt tacctaccat taaaaatagt gtgctcagag
    22441 tccaatattt caatcagatg caataacaga cagcatttta tttggtgtac tttaggtatt
    22501 ttcaattatg atttataaat ttggttttta aataaaccca atattatgaa aaactcattc
    22561 cgtattctgt tggttgtgct cacgactgct ttagccttta ccatcggttg taaagatcct
    22621 gaagaagaaa ccgaagccac gttcaatctg aaaatcaccg atgcccgtgt gcctcaggca
    22681 gctacgaaag atggagatgc acctgtgctt atcagcgatc agctcaccaa atgtgaggtt
    22741 acctatgccg atatcgaact aaaaaaagac gatggagagt acatttcgct catgacttcg
    22801 cctactactg ttgacctgag agattttcag gggacactgc acagtcttct ttcgataagc
    22861 ataccggttg gcagctatac gcatctgaaa gtaaccgttt cgggagtttc gacaacctat
    22921 cagaataaca actacacagc cagtgtcgac ggcggtgcca cagttaccct taacaatatt
    22981 ccgggtactt tcaccgaggc acaaggtgtt cccgatgtat tttcggcagg agccatcagc
    23041 tacgaggttc cgctggcatt cacgctgatt aactccgaag acgttgaatc agttcagttg
    23101 cagttcgatg tagatggttc tgtatatatc gtacccttca cctatcagtc gtttacctgg
    23161 aattttgcag ccctgcatac ctattttagt ctgggttatg ttttcgagca aggcatacaa
    23221 caaatacgcc attctccccc gctgggtatt accattgttt ctgcaaacga tgttgattat
    23281 tacggcatac atacttttgt agatttcgac gctcacggag gcaccatcaa ttcgcacaca
    23341 tcacaacacg ttttccgtgg cagcgacggc tccctgctag ttgatgccga agcaatgact
    23401 atcaacaaca atgtgctttc cccaaacaca gtgaatgcta ccggagaaag cgacatccgt
    23461 gccgatgaaa catttcatta tacagccatt ctgaataatc ttgccgggca gggatatact
    23521 cttctatcag gacacaccta ttattttagt ctgcgtaaaa cgtggaatat caccaccaat
    23581 ggtacaacct atgatttgac cagaatttgt gagcccattc cggtaactat tccataacta
    23641 tcaattacgt cattaggtgg ttttgcggta ttgccatact gccacagcca taatcactgt
    23701 gcccaaagct cccaaagagc ctattacggt tgcgatatca gccagagtag atccttttag
    23761 cataatcata cggatgcctt tcataaaata gaaaaccgga ttgatgcggt tcacttcctg
    23821 tgcccaatgt ggcatgcttt ccaccgaagt gaacaccccg ctcatcagaa taaagagcag
    23881 gaagaagaag aaaatggtaa acatcacctg ctgctgggtg cgggcaaaat cggtcaccag
    23941 gattcccagt ccgatggcag taaacagata cacgcaggta aacacaaaca aggttagcac
    24001 cgaacctgcg aaaggtaagc caaataagag catgccaatg ccaatgccca gacttaggtc
    24061 gaacaacccg ataacccaaa atggcaatat tttcccgata agaaactgaa attttcccac
    24121 cggactcacg ttaatctgct caatggttcc catttccttc tcgcggatga ggttgagggc
    24181 tgtgagaaaa ctacctatca tggtaaccag tatggtgaga atgccgggaa gcatgaaata
    24241 cttgaagttc agggtttcgt tgtatagaaa ccttggacgg atgtcgacag cagccggctt
    24301 aatcaccgcc tcctgctttg ttcctgcagc ctgattcacg gctgcggtta taatgcgctc
    24361 agcataaata ctaatcagtc cggcaccctg tccgtcgatg gcattgatat tcatttgcaa
    24421 gcctgtggca gcacccgttt taaggtcttt ttcaaaacca gcggggataa tgatcactgc
    24481 tttactgttg tcgtttttca gctcaccggc aatataatcg tgcgaatatt gtccgtcatt
    24541 gactgtgaaa aacggcgacg catcaaattt agatatgatt tgcttcgaaa gctgcgaatg
    24601 atcgagatcg ataatgctta caggggcatt ttttatttca agagtggcgg cgtaaacgag
    24661 tacaagcgtc tggatgagcg gcatcacaaa aatgatgggt aacatggtgc gattcctgaa
    24721 aatctgccgg aactccttct gcagtatgaa tagaattata cgcattactc cagccttgcc
    24781 ttaaattttt taatactcat tccgaggaaa aacagcgaca taccgagaag caccagggtg
    24841 ggtttccata catccgccaa ccctccgcct ttcagcataa tcattttaat aataagcaga
    24901 aaatattttg caggtataat cacactcaac cactgcaaca ccaccggcat attctcgata
    24961 gggaaaataa aaccagataa caataatgtg ggtaacatca gtacaaacat cgaaataaac
    25021 atggccatca tctggctctt cactgcactg ctgataaaaa tgccaaagca cagcgccaga
    25081 aaaatgaaca gcagtgtttc accgaacaac aaccacaggc tcccatgaat tggcactcca
    25141 aacactaggt ttccgattgc aattaccgaa catgcattta caacagatat gagaatatag
    25201 ggtgtaactt tcccgaaaat aatctgccat cggtttagcg gtgaagcgag caagacttcc
    25261 atagtgccat tctccttttc gcgaacaatc gaaatagagc tcatcatggc tgtaataagc
    25321 atcagtatca gtgccatggt tcccggtacg aacatataag aacttttcag gttttcgttg
    25381 taccacatac gcgtttcgac tgaaggccgg tgcaggctaa taccgttgtt attcaacccc
    25441 gcgatatatt tcattatcat cgcagtggtg tagctgttaa taaggtttgc catattgggg
    25501 tctgatgcat caagcatcag ttgtattgta gcccctgagg atgaatgcaa atccttatca
    25561 aaacccgaac cgataacgat tacctctttt acttcggacc tacgaaatgc ttcttcggcc
    25621 tcaggatatg aataatatct ggataccact tcgaaatcgg ctgaagcatc gatacggtta
    25681 atcatttcaa cagcatgggg gctgcccgag aaatcgagaa cacctacttt tacatcctga
    25741 atttcgtttt ttattacata cccgaatatt agtatttgga atacaggaat agcaaagagt
    25801 atcatcattg tccgtatgtc gcggaagatg tggataaact ccttgattaa aaatcctgcg
    25861 aagcgtttca tagcaattta ttttcgggcc agttttaaaa acacatcctc caccctttca
    25921 acaccaaatg ctgccttcag gtttacaggg gtatcgaggg catcaattcg tccgtccacc
    25981 ataatcgaca ccctgtcgca gtattctgct tcgtccatat aatgcgttgt aataaaaacg
    26041 gttataccac ctgcggcagc tttaccaatc atatcccaga attggcggcg tgttacgggg
    26101 tcgactccac tggtgggttc gtcgagaaaa acaatacgcg gttggtgaaa cacagccacc
    26161 gaaaaagcga gtttctgcct ccatcccagc ggtaacgacg ctatcaggct atcgccaaga
    26221 tgagtaatgc gcagttcttc gagcagttct tctgttcgct tgcggatata gtcgcggctg
    26281 agtccgtata ttccggcaaa aaaacggata ttttcgcgaa tggtcaggtc ttcgtacaac
    26341 gaaaatttct gactcatata accgatattt ttcttgatct tctctcgctg cctgtacaca
    26401 tcgaaacccg ctaccataat gttacccgaa gtgggatacg acaaaccaca caatattttt
    26461 atcgcggtgg ttttaccggc accgttagcg cccagaaaac caaaaatctc gcctttatac
    26521 acctcaaaat tcagattatc gttggcaata aagtttccaa actttttcac caggttttcg
    26581 gcttttatga tgatttctcg atctgttttc attttgtcat cagttggagg aacacatctt
    26641 cgattgatgc ttcaccctgc tcaacattaa aattttcaaa gccacgcgaa ttcagatggt
    26701 tcgctatttc gtccacacta agttttccgt gcggtgcagc cacatgtatt tcttttccga
    26761 aagcatatgc cgacaatacc tccggtatct gccgcagcga ttccagacac cggaacgcat
    26821 cagaatcggt taccacaaaa agcgcatcac caaacgacga tgaaatcgac gctgtgctgc
    26881 caatagccat catggcacca ccctgcatca gggctaccct atcgcatttt ccggcatctt
    26941 ccatataggg tgtcgaaaca atgatggtaa gaccctgact ttggaggcgc ttgagcattt
    27001 cccagaactc cttgcgtgaa acagcatcta cgccggtagt aggctcgtcg aggataagta
    27061 tttcgggttt atgtatcagc gcacaacata gtgcaagttt ctgcttcatt ccaccggaga
    27121 gctttccggc acgacgggta cggaatggct ctatctgtac ataaatatct cgtataagat
    27181 ggtagttttc gtgaatggtg gtgccaaaaa cagtagcaaa aaaattcaga ttttcctcaa
    27241 cactgaggtc ctgatacaac gaaaaacggc cgggcatgta acccagaatt tttctaaccg
    27301 atctgtaatc gactttaata tccagcccat tgagtgtaac actgcctgta tcggggagta
    27361 acaaactggc cataatacga agaagcgtgg ttttgccggc gccgtcaggt ccgatcaaac
    27421 cgaaaaactc gttctgctga atactgaaac tcagattttt aagagcctct accttcttat
    27481 agtttttcga tacctctgca acttcgatgt atgccatact tatttattat taaaatttac
    27541 ctcagcaggc atgcctattt tcagttctcc tgtattggga acgtatattt ttactgcgta
    27601 aacaagattt accctttctt cgtgggtctg gataattttt ggcgtgaatt cagcttcatc
    27661 actgatccaa gcaatagtac ccctgcaagt cgctttttcg gttacagttt tatcgtagag
    27721 cacttccact gtatcgccca atgctatatt atcgagttgc actgcactta cataggcacg
    27781 aagggtaagg gtactcatat cggctatttc gcaaattggt ttaccctgaa cagccatttc
    27841 atttgcctgc atatattttt caagaacaac accattcaat ggactgataa ttaccgaccg
    27901 ctgcagttgt tcattgagaa tggcaatctg tgtatcgatt acatcgagct ctgcaaacac
    27961 gcctgcattt tgcgatttca ggctttctat ctgcttacgg cttacattta cctgagatgt
    28021 aatgtcgtcg tactgtttct gggttgctac attgttcttc agcaagtcgg ctacacgctg
    28081 ctgctccttc aaaagactgc tcagctgttc ttcggcaaca gccacctgat tgattatatt
    28141 atcgaattta acccgaacgg catctttctg ggttattagc tgttttcgct gtaatactag
    28201 aatagcagtg tcgacaactg caatggtgtc gcctttgcga atctgcttcc cgtcttctac
    28261 aggcataaaa atgattttgc ccgatgtttc ggcagaaatc accgtacttt cggtttcgaa
    28321 attgccatag gcatcggctt tctcgccatt gccaccacac gatatcagca tagtaattgc
    28381 cgccgctcca aaacaaagca tgactattgt tgcgttattt ttcatacatg attcctttta
    28441 aaagattata tttaactttc gcttccgcaa gcataataat gtgcaggtta taattgccct
    28501 gagcagcttg ctcatcgttc aatgcattca aatagtcggt ggaagttgct gttccacagt
    28561 taagtcgcaa tgaaacggct tcggtaatat tgtgcttcag ggtaatgatt tcttcgtctt
    28621 tttccataag tttttcgcac ttttctatcc gttgcatttg ggcatcaagt tgtgactgct
    28681 gcaggtagtt gatctgttca atctgtgtat caatgatgct ctgttgcact tgtatctgtt
    28741 gcctgtccga tttggattta ccccagtccc atggtgtcca taccatcttt gcaccaacaa
    28801 tcacaaaagg ttcaaattcg tttgaaagca tattcagtcc tggccggcca taaccaagct
    28861 gtgagaaagc aaacaccttt ggcataagcg aagttttaag cattttatcg ctcgacgata
    28921 attgattttg catcgcatga agataagttt catcactcgt ggtaataccc gtctgcaacg
    28981 acaattccgg ggaaggtagc aatagcatag catcttcaga aatgggaaca tccatcaatc
    29041 gaccaagcat tttttttgaa gcagcaattt cataatcaag ttcaatgatt ttctgctcta
    29101 ccacaagttc ttctgcattc agcatatcga tagtttcctt taacaccaca cctgaggctg
    29161 ctgctttatt taggtcttcg actttcagca gcaactgcca ttttttcacc atcaacactt
    29221 cctgctgact ttgcagcttc aggatggcaa aatagaggtc aactatctgt tctttaaaat
    29281 ggaacatgtc ctttttaatc ttttcggttt cgctgacaac tgcagctctt tcgtagtcaa
    29341 gcattgcagc tgtcacacca ccatcccata tattctgcct caggtcgaga ctaattctgt
    29401 actgatcttt cgaaatatcc gtcaaggact ggggcataat gggtaagtcg atgtcaatgt
    29461 ttgtaacttc gttttgccat gttgcctgag catttagtgt aagcgaagga taccagcttg
    29521 ttttcaggct tttctccttg aaaatatagt tttcttcggt caaaccgaaa agtcgtattg
    29581 cagggttgtt tctttcagca gcattcaggc atgaatcaag gctcaacacg tttttctgtc
    29641 ccatcgttgg gaaaagcacg gcaataaaca acccgataat caatagtttt ttcatgaggc
    29701 aaagattgat tttaatacaa attcgggaac acggtctttt ctttcattaa gcaacatttc
    29761 gtattcttct ggttgcaagt ccagaagcat agtaatcatg tttcgggcca caaccgggaa
    29821 aataagcatc gaaataatat taatcatcag atgcacttcg ctttgcacat gaatctcgtt
    29881 tttttcggcc gattgatgaa ccagcgattt gagcagcgca aaacgggcct tgagcttcaa
    29941 atccgtcatt gtttcagcta tcagttgcgg gtggagtttt atctcggtaa ggagaaataa
    30001 aggcagttgt ggacgttgaa tcagaaaatc aaggtaggcc gtacagaatt tttttagctt
    30061 ttcttcgaaa gggatttcct cctgaaatac acccgaaatg ctttcaaaca ttgcccctac
    30121 cgtatcggca aatactttct tgaacaggcg gtctttactt cggtaatagt aatgcagcag
    30181 cgatttatta atccccgccc tgtcggcaat ttcctgcata cgagcgccat cgtagcccct
    30241 ttccaggaat accgcgacag ctgcatcata tattcgttgc tcggcacttt cgtttatttc
    30301 caagcttttc atcgcattaa ccaaattatt taactatttc gtttaaccat taggtcaaat
    30361 ttataaaata attactataa caacaaaatg tttttaacat tatttttaac aattgaagaa
    30421 gttcattaat tttgaaccca aaaaaataaa acataatttt ttatgtataa tttcgttaca
    30481 agcaattagg atggtagtaa acaagcatat ttttgttttt accctgataa taacgtttat
    30541 catggcaata cctgttgttg ccagtgctca ggataaaaaa cttaaaaaag ccgatgaaat
    30601 atttgctacc ggggagtatt accgtgcact ggagttatat cagggtctta tccgtaaggt
    30661 taaagacaag cgggtgaagc aggaattgta ttttaagtcg ggagaatgct atttttacat
    30721 caataatttc aagaaagcaa gggccaatta caaaaaagcg gtgaagcatc cagagttggg
    30781 agttgatgcc tattacaaat tgggcttaac ttcgaaactt accggggatt acgaacaagc
    30841 gatacttgat tttaaagaag ccctgagtat ttaccctggc gattcgttgt cgcaacaagg
    30901 gctggagtcg tgtgaactgt cggtaaagtg gatgcaggag cgttcgcggt ttgaagccga
    30961 aaaatttaag gaagccaata gtcgtgaaaa cgatttttgc cctacggttg ttgaaaataa
    31021 cgggtatcct cacctctatt tctcttcaac gcgaaaagaa gccatgggaa agaagaccag
    31081 tggaattacc ggggagaagt tttccgattt atttgtggta aaatttggca aacagggaaa
    31141 atggagcaaa gtgaatgctc ttgattcgat aaataccatt tacgatgaag ggtcagccac
    31201 ttttgttaac gatggcagag atcttttcta tacctcctgc gtaaaagaaa aaggaaagaa
    31261 actcggatgt caggtttata aagcccggaa agaatccatt gagatgggta acaacatcga
    31321 cgtacaatgg atgaatccgg agcgtgtaga tatacttccc gacagtattt cgttcggaca
    31381 tccggctttt agtcccgacg gtaccatcat gtacttttct tcgcgtaaag atggtggttt
    31441 tggcggtgcc gatttgtggt attcggagca ggaaggcagc acctggagca aaccaaaaaa
    31501 tatggggccc ataataaata ccaaaggtga cgaacttttt cctttcatga gagctgacgg
    31561 agtgttatat ttcagtagcg accggcatcc gagcatggga ggactggata tttttaaagc
    31621 ctataaagac gaaaataaaa aatgggttgt agttaacatg aaaccccctt tcaacagcaa
    31681 cgggaatgat tttgggcttt attattttgc tggtgaagag aaagggtatt ttacttccga
    31741 tcgaaaaggt tcacaaaaag aggatattta ttcatttgta aaacccatac ttaaaattga
    31801 attcaagggg caggttttcg acctcgacac caagcaaatt gccgattcag cgattattac
    31861 cctttactgc ataaacggcc cgacaaagtc cgatacaata agccttgcag aagaaggaag
    31921 attccagttc gagttaaagg aaaactccga gtattcgttt tctgttgaga aagaaggcta
    31981 ttttcgtgga agaggcaggt tacgtaccga cactcttgac tttgattaca cattcgaaga
    32041 aatgatttat atcgaaaatt ataacaaaac cgtcgagatt ccgaatattg aatttgagtt
    32101 tggcaaatac tccattactg aagcatcgaa aagctcactg aacagtctta taaaacttat
    32161 gcaggataat gccaatttgg ttattgagct ttcagcgcac accgatatgg ttggaaccga
    32221 tgaatcgaat atggagctta gcatcaagcg tattgaaagt gtaaaacagt atttaataga
    32281 aaaaggtatt aatagcgaaa gaatcaaagg agtaggttac ggggaatcaa agccaaaaca
    32341 aataagaacc tatacaccag aacatccatg gctgcaaata ggaacagtgc ttgacgaaac
    32401 ttatatttta tcgctcgaca aggaaaaaca ggaaattgct aaccaaatga accgtcgtat
    32461 tgaattccgt gtaatatcga ataaatatga acccggtctt gattaaaatg ttggtatgat
    32521 tttagcaata acacactagt taataattaa atataattac aactttgaac cagattgagg
    32581 cgattaaaaa gatgataaaa gattttctct ttgcaaccaa gctgtcaatc gaaaacttat
    32641 aaggaaacct aaaatgaaaa cgctggcaac tatattactg agttttatta tgattttgct
    32701 gtggacatac ccctcctcag cccaggtaaa aaacggttat cacgataaac ttgctgcctt
    32761 atatgaagaa ggcgaatatc tcgattgtgc tttcaaagct gaaagaatgc ttatgaaaga
    32821 tgaatatctg agcgaccctg aagtgtacct atatcttgcc atcagttaca acaagatata
    32881 tttaatgact aaggaggacc caacaattat tcaggatgaa ccagagtatg cgaaagcgta
    32941 tgataacgca ctaaagtatg cagctgattc aaaaaagaaa gataaaaaag ccaaagaatt
    33001 ctttcccgac aacaacgatt tgctggaaga aatagtttat accggtttac ccattgccga
    33061 gcagtatata tttgaaaaga aatactccaa agcccttgcc ctgtaccgca agtttcttaa
    33121 gttgattgat aatctccata tcagctttat aaaaggagtt atggacttat ataccttgga
    33181 caaagcaact gcaaacgaag aattcaaaaa agtatttgct gccatcgagc agaacagcct
    33241 taccactaat gacaatacaa aatatctgat gaaaacaggg ttcctgatgt actacgattt
    33301 tctgttgcaa gaagactctg ttgcaaacgc tgatagcgca aagcatgtat tgcgttacgc
    33361 agtacgactc ttccccgaag accgtgaaat aaaccaacgc tttttcccaa gttcaaaaca
    33421 atagaattta tgattggaat aatcggtggg acaggattag aaaggcctga ttttttacaa
    33481 tttacaagcg aactggaaat caatactgct tttggaagcc cctcatcacc ggtaaaatta
    33541 gccgaatttg ggggcaaaaa aatagcattt atttcgaggc atggcgaaaa ccataacatt
    33601 accccaaccc atgtgaataa tgcggcaaac atcctcgccc ttcagcagac aggatgcaac
    33661 tgtattattg ctaccacagc gtgtggtagc ctgcgtgaag aaattggaag aggagatttg
    33721 gtgattgttg accagttcat tgattttacc cgtcaccggc gggttagctt ttacgactca
    33781 ttcgacaacg gtgaaatgaa acatacagcc atggcagagc ccttcgatta caatttaagg
    33841 aaaatgctag ccgaagccgc acggcaaagc ggtatcaatg tgcacacttc tggaacagtt
    33901 attactattg aaggcccacg tttttcgact cgagctgagt cgaatatgtt tcgcatctgg
    33961 ggagccgata ttattaatat gagcacggca cccgaagcaa ttcttgcaaa cgaggcagcc
    34021 attccttatg cagttgtagc actttcaacg gattacgatt gctggaaaac caatgaagaa
    34081 cccgtgagca tcgaagaggt aatacacact ttccggacaa atatacccag acttattcag
    34141 cttctttcaa cttttatttc cggttatatt gagcggtagc ttgttcttca taatattttg
    34201 aaaataattc gtaaagacat gcatgaatcc gggaacttaa ctgcatacga agtaatcaga
    34261 atcatcggat ccagacctct tttcctgaat gagcatctcg ataggcttca gagatcgctt
    34321 ttcgatatag ataaagattg tattcttccg gttacattgc gcggtgagtt tattgctttt
    34381 gtgcaaaaaa ctggtgtagt tgacgggaat atcagaattg atgcatggtt caccgaaaca
    34441 acgcaatacc gtttttatca gattccacat cactatccta cggatcaaga gttctgcaac
    34501 ggagtttcag cagttaccct tattgcagag cgcaataacc cagaaagtaa aatatggaac
    34561 aaagaattgc ggagcaacgc gaactcatta atagaatctt caggtgtatg ggaagtattg
    34621 cttgttaatc agaagggcaa cattactgaa ggcagtcgct caaatttctt tgcaattagc
    34681 ggatcaacca ttgtttcggc tcccaaagag caaattctgg gaggcattac aagaaaatat
    34741 gtactcgaag caatacgtag tgctgataat gttgactata tagaacagga catcccttta
    34801 cacagcatta aaaactttga tgccgcattt atcaccggta cttcaattaa agtgttacct
    34861 ctgaaaagta tcgacggaca atacttaaat gtaaataatt tgattttatc gcaaataatt
    34921 tccaattttg accatataat gcaacgggag ttaaaccgaa attatgaaat gtaacaaaac
    34981 atcgattgtt gttgtattgc ttgttttggc attctgcaca ggtgctacag ctcaggactg
    35041 gaaaaattgc tacgattatc ccggaattaa aatcagctac atcagcgata cggttaaaaa
    35101 tgtgtataag gtcagagttt catctttgtc gtcaatcaca atgaatgagc tctataaacg
    35161 cctactagat gtaaaaaaat accccgacta tgtaacaaac tgcatttatg ccgaaacatt
    35221 gaagaaatat tccgacagtt gcttcattta ctacaccata acagagctcc ctttcccact
    35281 aaagaaaaga gatattgtcg taaagctcac gtcaaagaaa tcagcgggtg cttttactat
    35341 tgttacagaa gtttttaata atttcctcga agaaaagcag ggctacgaca gggttaaagg
    35401 ttacaaagcc atatggtttg ttgaagaaac aaatgcaggt ctcgaaatta aacttagcat
    35461 ggaattaagc gtaccacaaa tagtaccggc atggctcagg tatcagatta ttgtgaaagg
    35521 accggtagag acctatctca ggttcacaca ccattaattt ctattaaact gcaaagtatc
    35581 aattattaca atcaaatatt tccttggctt ttacgtatga gtaaaatatg attaaactgt
    35641 tatccatatt ccgaagtgca atcatatggc cattcacaat attgataaaa atatatcaag
    35701 taatcatttc tcccttgttg cccaagtcgt gtcgacatta tcccacctgc tcggcatata
    35761 caatacaggc actaaaaaag cacggtttaa ttgccggagg agtgctttca acatggcgaa
    35821 tcctgcgctg caacccatgg ggtacacacg ggtatgaccc tgttcccgac aaaattatct
    35881 tcagacgcca gaggcatatc gacaaattaa ataactcatt acaagaagat agttttaagc
    35941 agcctgatat taagcgcaac aataatcagg tggggcagat gctgacaata tacctgatag
    36001 caggagccgg catgttgtcc ggttgccata agaataccga acaaccccct cccccgactg
    36061 caaccgctta cgacattatt ttggttattg gtcagtcgaa tacgcattac ggagaaggct
    36121 acgacacgga tttggattgc ccggaaagcg gcatctggca acttgggcga tttggcgcaa
    36181 acaacttcaa gattattgaa gcctcagaac ctttggaaca ctataccagc caacccggct
    36241 tcatcgggtt cgccttaagt ttcgccaaat actacaaaaa aacgttttta cataccaccc
    36301 gacaagttct gataattccc tgtggcaaag gaggaaccgg attcaccgac aaatcatgga
    36361 accctggtga ccccctctat gaggatgcag tatcgcggac aaattttatc atcagaaact
    36421 acccgaactc gaaactagtc gctattcttt ggcatcaagg ggaaagagac gaattcaacc
    36481 agaactacaa agaagctctt gattcgatga taatccggat tagaatggat atttccggct
    36541 gtagtgcttc tacacccttt attttgggag gcatggtacc ctactggaca aataaagccc
    36601 aaagcagggt cgaaataaac aacattattg ccggaactcc gaaccggatt caaaataccg
    36661 gttatgccga tccacgaaaa ccatatatca tcgaaaaacc agataattct tacagcgaaa
    36721 tacatttcga tgcagcaggg caaagagaac taggcagaag gtattttgtt gtatttagct
    36781 caatggtaag gcctcagtaa ttcgtaaaaa tatcattttc aaccctgatt attacaattg
    36841 gatacaactt tgtcaggaaa aaaatacact atatttgtaa ataaccaaaa cgccacaaac
    36901 accatgaaaa tgaaatttca gttttttatg tttgctacct gtattgccac aggattattt
    36961 gcccagactc aggtaaatcc caaaatcatg gaggtaactg ttttttcaaa tgccgccgaa
    37021 ataaagtcga ttgcttcggt aaatgccggt gcgggaataa ccagtatagt atgtttggac
    37081 atctcgggga atatgcaggt gaacagttta aagcttaacc cttcggaagg tgtcgaaatc
    37141 acagggttta gcgttgaatc gtattatttg ctaccggaaa ccctaatacc ggaatacaag
    37201 agaatgaatg attccattac aatgttgaat aacactatta ttacactacg taataagcaa
    37261 gaggcgtatc aggctgagaa aaacctgctc atggcaaatc agtcgatagg aggcgaccaa
    37321 aatggtgtta ccctgcagga attaaaaagc atctccgatt tctatcgcga aagattgttg
    37381 gcaattaata acgagctttc gaaacttact gatgaaatat cgaagctgga aaagcaaatg
    37441 tcggagataa gcaatcgcat gacaattgca tcgtataagc atgaatcatc gcgcaaaaaa
    37501 gtgtacattg atatcagctc agaatcggca gctaaagttg aaatcgaact ccggtatctg
    37561 gttggcaaat gcggctgggc tgcaacatac gacctgattg cagaagatat caatatgccc
    37621 gtaaaactga aatacaaagc caatgttctg aacaattcag gaatcgactg gaacaatgtt
    37681 cagctcattc tctccacact cgacccatta aaaggagcat cagcccctgt gctaacaccc
    37741 tggtatctcg actttacggc agatgaatac aattatcagg accagtaccg ctatagccgg
    37801 tcatcgaacg aaatagaata caagagcgaa caaatgaccc ccgggcaaat tatggatccc
    37861 aatcaggctt atgataacat cagcgtttcg gagctttcaa ccgaattcat aatcaacagg
    37921 aaatacagta tccccagtga tcagaaacca tttaaaatcg acattacaga atacgacctc
    37981 aatgcagaat acaagtatgt aaccattccc aaactcgaaa ccaaagccta tttgcttgcc
    38041 cgcattaccg gttgggagaa atatgaacta atcgacggtg atatgaatgt ttattactca
    38101 aactcataca ttggagtcag ccggctaaat accaatacag taaatgatac cctcgattta
    38161 tcgcttggtc gcgacaataa aattatggtt acccgtataa aaatggaaga tttcagcagc
    38221 aaatcgttcc ttggcaataa ccgaaaagaa tcgaacactt accaaataca aattaagaat
    38281 aacaagggta ctcctgtaaa cattgaggtt gttgatcaga tacccgtgag tcaggaaagt
    38341 gaaattgaag ttggtgtgaa cgaaatatca gcaggagcct ataatccgat aaccggactc
    38401 gtaaagtgga acaccgagtt aaaacccgcc acatcgcaga aatttaccat ttcgtactgg
    38461 gttaaatatc ccaaaaacaa gccggtgcaa atgagccgga aacgtgctgt agctgccccg
    38521 cgtttctagc ctgaccggta agaatgcgaa acatactttg atgcaagaaa aatctcagca
    38581 catattaaat attattcctg aacagggtat atcataacgt gaattatata tttttgagca
    38641 aaatttttaa aagatgaatt tattgcttca ggttgtttgg gatgttgacc ccattatggt
    38701 tgacctgggc ctatttgcgg taagatatta tagtctgcta tttgctcttg gctttgttat
    38761 cagttatttc attattaaaa aaatatttaa aaaagaagga actcccattg aagagctcga
    38821 cagactattg atttacgtgg tagtcggtgg gctggtcggg gcaagaatta tacattgtgt
    38881 cttttatgaa gccgattact ttttatcagg attttttcca tttctcgaga tattcttccc
    38941 tgtaagcttt tcacctgaat tcgaatttat cggataccag ggattggcaa gtcatggtgg
    39001 ggcgttgggc attatcattg ccatactaat tttcaaaaac aaatcgaaga ataagagttt
    39061 tttctatctg atcgacaggg tggccatacc tacaggtttt gctggagcat gtatcagact
    39121 tgggaattta ttcaacagcg aaatatatgg agaaaaaaca tcactgccct ggggatttgt
    39181 ctttgtaaaa aataatgaaa gtgagcctat gcatcctacc cagctttatg aagccacatt
    39241 ctatataata acatccatag ttttgctttt gctttacaga gtagagcgat tccggaatgc
    39301 aaggggtttt ctgctcggaa tgtttctgat gatggtattt gggagccggt tacttattga
    39361 gttcataaaa cacattcagg ttgggttcga agagaatttg gctttgaaca tggggcaaat
    39421 gctcagtatc ccgtttattc ttgcaggcgg ctttctggta tggtactcct taaaaatgga
    39481 tggtcatact gttgatgtaa aaaaaaataa ttaaaatctg ttgtgctaat cccatcagat
    39541 taatttacat ttgcatataa acaaggctat tttatgtggc gactcacgaa agagcttttt
    39601 aaaaggtaca ttcgtgcaat tttactgcta acaggggtat ttctgctcat tctgctgggc
    39661 atccttgcca ttgagttcgg agccggacac tccacaatac acaattattt cgaagctatt
    39721 tggtatggct ttgttaccat tgccggtgtt ggatatggcg attatacgcc agtaacaacc
    39781 ggaggcagaa ttcttgggat gctgctcatc attggcagct tattgcttgt tgggattttt
    39841 accggtcaga taacaaacga aatcagaaaa attatggaag acaaaaaaat gggtatttac
    39901 ggtaccaaaa tgaccggcca ttttgtaatt atcggctggg acagttttgc ctcaaatgtt
    39961 gccgaatggg tcctcgatgc cggcaaagac ctcgcaatta ttaccaataa tccggccgac
    40021 atcgacctga tatacaatcg ttttggaaat aaaaaagtgt ttgcattgtt tagcgatttc
    40081 gataagcccg aaagcttcga tctggtaaac attgataaaa gtgcttctgt ttttataaat
    40141 ttcaaggaag attccgaaac attggttcat ctgattaata ttcgaaaaaa atatccacgg
    40201 ctgaattatg ttgcctcact caacaactca caactcaaag acacttttca tgctgcaggt
    40261 attacgttta gcgtatcgaa caacgaagta gcctcgaagc ttgtcgccag ttatattttt
    40321 gaacccgatg tggcaaactt caccgaagat ataatggcct ctgcaaacat gaacgacgat
    40381 tacgacatgg ttgaatatca ggttttttcg aacaatccgt atgccaataa agattattat
    40441 gaagcttttt tcgacctcaa aactaaatac aattgtgtat tacttggcat cagtcgcaaa
    40501 ataggggaaa aatataccat aattaaaaac ccgaagaaag gcgaaataat caggaatgcg
    40561 gattttctga ttatgcttgc cgacggcagc acaaaaccac agattgagca gttgtttggc
    40621 actaaagaag ggcggtgcgt actaaaaagc taatccttgt ttttaaggaa caaaataaga
    40681 ttgtcgataa ctttttcatc aggttcaaaa tcaatatttt ttaactcatt acccaaattg
    40741 aagtctgcaa tctgattaca atcaataaca attttttccg gctcggctga attgttgtat
    40801 aataccagta gcgtagaatg ttgaatcata ggcagtttat tttagttata acgctgtgat
    40861 tttgttatta ttatattatg gataaagaaa aaattatcat cttccaaaat gatttaactt
    40921 tgcattcgtt attgaaagaa tgagaaactg gattggagaa catataaatg ctatactagg
    40981 aacaattcta gcccatctca ttattgtttt catcgccctt gtacttaaaa tcaataccag
    41041 tgtagtgcat agcgaaacgc ccataataat tgatacagag ctacttgccg aaatgcagaa
    41101 cattccggaa gagaacagtt ccgaggtaaa cgacatggta aatgttgatg agtatttatc
    41161 gaaactaact tccgccagca gtaattatag tggaatgaac aggagtgcaa acgacaacct
    41221 gcacgatagt aaaaacctaa gcgttgacca aatcaggcaa atgtatgaag aagaaattct
    41281 cagggaaaaa tatggagatg attatgaatc gcgatacgag gaatcggatt atacctatac
    41341 aactccttat gctattcctg ataaatatcg cgccgaaaac acacaggggc agcaaatcga
    41401 caatacaaac aaaccttgcc tagtgaaagc tgtactcgat gaccctacac gtgcgaccag
    41461 atacttgcac atacccgttt ttacgtgtca gggcagcggg gtaatcgaaa ttgcgattgt
    41521 tatttctccc gaaggcaagg ttatcaattc gaaactgctc agtacgaata ccagtaatga
    41581 tcaggattgc cttgttgaag ctgcgctcac tgcttcccga aaaagtcgtt ttggcattat
    41641 aacatcaaaa cagaatcaga acggcaaaat tatctacaac tttgtaaatc aataattgta
    41701 aaattttaga atttctattt cagaattatt aattttacga ttaaaacaat accacaatga
    41761 tacaagaaaa gctggtttca tatttcgata acagcatacg cgaaaactgg aataaaacag
    41821 cgcttcgcga ttacgacaac ggcgaaataa gttatgctaa aaccggtaac accattttga
    41881 aacttcacga tgttttacgt agatcaggaa tagttgaagg tgacaagatt gctgtaattg
    41941 gtaaaaattc agccaactgg gcttgcgtat atattgccgc tgtaagttat ggtgctgtta
    42001 ttgtacctct gctacaggat ttcaccgcaa aagaggttta caaccttgta aatcattccg
    42061 agtcgaaatt actgtttgcc tcggatttca ttttcaacaa attgaacctt caggagttta
    42121 cgcttttaaa gtctatttat cagctcgaca attttgagct attattcgat gcagatcaaa
    42181 aatcaattcg ttttaacaat gcacttcccg aggtttctca ggctgatttt gctttacata
    42241 ttcccgaaaa ctctttattg gcggccatat tatatacttc tggaacttcg ggcaacccca
    42301 aaggtgtaat gcttgcccac aatgcactgg cagcaaatat acgttatgca agggcaaaca
    42361 tgccgcttca gcccggcgat agaattgttt cgttccttcc ccttgcacac agttacggat
    42421 gtgcttttga gtttctgtac cccttctcaa taggatgcac catcacattc cttggtcggc
    42481 tacctgctcc aaacattgtt gttaaagcgt tcaacgagat aaaaccgcgg ctcattctta
    42541 gcgttcccct ggtaatagaa aaagtttata aaaccaaaat caagcctaag ctttcgaaac
    42601 cgggtattaa gttgatgctt aaaatccccg gcctcaggca aattgtttac cggaaaatca
    42661 gagctggact ttctgcatca tttggaaatg aatttatgga ggtggtcatt ggtggggcca
    42721 aattgaaccg tgaagtggaa atattccttc ggaaaatcaa tttcaacttc tctattggat
    42781 atggtatgac agaatgtggg cctctcatta cttatgatgg atggaaaacc acacgaacct
    42841 tttctgccgg aaaagtaatt gactgcctcg aaatgaaaat tgattccgat aacccgtcga
    42901 agcgtgttgg tgaaatattg cttcgtggag aaaatgtaat gctcggctat tacaaaaata
    42961 aggatgaaac cgaaaaaaca tttaccgagg acggatggat gaaaaccgga gatttggggc
    43021 ttattgatga agatggatat ctttatataa aaggcagaag caaaagcatg attctgggcg
    43081 cttcaggcaa gaatatttac cccgaagaag ttgaggctaa gctgcagaat ctacctttgg
    43141 ttagcgagtg tattgttgtt ggcaggcaaa atcaaattgt ggcaatggta tatcccgatc
    43201 atgccattat tgaggaacgt gctttaaaca aagagcaact tgaaacaatt ttcagcaatt
    43261 atcagaaact ggtgaacaaa gacttgcccg gatatatgca aatacaaaaa attgtgctac
    43321 gcgaggagga atttgaaaaa acacctaaaa aaagcattaa gcgttatttg tatgaataaa
    43381 taagggtttt attttttctt ttgtctttcg aaatagcgca ccagtgcctg attgaaataa
    43441 taatgatttt cgatgcagaa atacacatca ttgtttattt ctataacagg ataccttact
    43501 gagtacccct tacccctgtc ggcaaccgaa attgatgcag taattttttt atcgagtact
    43561 acattgttct cttcattatc gaggtcgatt attttaaaag gaatcccggc ggtttcgtaa
    43621 agctcaacag catctttaca cctgccacaa tcgagatgta cataaacatt gatttgtgta
    43681 attccgtctg tatattttaa tgaatcgaaa cgctgatagg gtatttgatc gggccggttc
    43741 tgttgtgcaa gtgccatcaa cgagagtgca aacattgtaa gaatcaggta aatctttctc
    43801 atatttattt ttttttgcaa tttacaaaaa tcgaaccaaa atcagaagtt gattttaatt
    43861 ttcagattta gtctgcggcc tgtaagataa ttgggcactg catacattct tccgtaaata
    43921 tccgaaaccc agatatgcga aatggtgttg ttaatatcga gtaggttgaa cacttcgaga
    43981 gtaatccaaa aatctttaat aaaccgaaat ggatttgatt ctgacagtgg cttgtttccg
    44041 atgagttgct ttgatatacc aaggtcaacc ctgcgatagg gtggaatttt tccggttgcc
    44101 tgaaagcgtt gcgaattagg aggaccaaaa gaaaagccag taccaaaaag caaattcaga
    44161 tgggcttgcc acgtagggtt attcggaagg taatcgtgga agtaaatggc aaaattaaat
    44221 cgttggtcag tcggacgacg tatgtatccc ggaaaaatgg tgtcggtctg accggattgg
    44281 ctttcaacag tatagaagtc attatcgata tcttccatgg tcttcatcag cgacatactc
    44341 aaccacgatt ctgtgccttt aacaaattct ccgttaatct tggcttctat tcccacagca
    44401 tacccatgag ccatgttttc gccataatat ttaatcctca cattatctac atcataagga
    44461 ataatattat acagatattt gtaatatact tcggtaacca gtttaaacgg ccggttccag
    44521 gcaagaaact gatagtcgct gctaataaca gcctgaatcg atgattgagc tttaatatcc
    44581 tcatttattg tgccatcaag ccgacgtatt tccttaaaaa acggaggctg ttgataaagt
    44641 cctgtcgaaa aacggaacac aaagtccctt ttccatttag ggtcgtatga aaccaccatt
    44701 cgtggactaa ttaaagcctg attattaaag ctccagtacg aaccacgagc acctgcattc
    44761 agtccaattt cgccttcgcc ggctttaaag ctataggtat tctgaaaata agcttctacc
    44821 ctttcggaag ccatcccaac aaccgatgta tcggtaaaga acagcataac caggctatcg
    44881 gtatagggta gtgaatatcc tgcagaatca atcatctgcc actctttcac agaataatca
    44941 aaaatctcat gtttgtagca ggcgccccaa ctcattaaat tatcgttatg ccggtaatcg
    45001 cccttgtacg aaaaggaaat aacataacca tcgaggtaat ttcgtgcatg attgagaaac
    45061 gatcccaccc cgatattcga tatactgtct cccagattat cagaccccaa ctctttatcc
    45121 aattcgttta aataatattg ccccagaatg tcgtaagttt cttcttcgct ggtgcggaat
    45181 gccgaagcaa taaacttcat gtccacgttt ttattcagct tatatttacc ggtcagtgct
    45241 cctgtaagcg taacaaattt atccacctcc tggccctcga aatacatttt tatttttaat
    45301 gcttcatcga gcgtacccca cgacgtttcg cggtcttcag gaataaatcc gtaataatta
    45361 ttactataat tacctaaaaa gctgatttca aatttctcgg tcagatcata cgaaagatag
    45421 gtctgaaaat cggtaaaacg cgggtcaaac agggcttctg tatccatgct acccaacaga
    45481 tactttgttg ttttatatct gaatcccgtg atgtgcctga atctgcgatc tttcgaaagt
    45541 ccctcaaagt aagcatttac accgagaaaa ctggccgtaa gcgaagcctt ccattcaacc
    45601 ggtttacgat aggtaatatc gagaaccgac gaaattttat cgccgtaacg ggcttcaaaa
    45661 ccacccgaag agaaaagcac cgacgacacc atatcggaat tgataaaact cattccttcc
    45721 tgttgtcctg accttattaa tagtggccgg tagatttcaa tgtcgttgac atacacaaga
    45781 ttctcgtcaa aatttcctcc acgaaccgaa tattgtgagc ttagttcatt attcgaaact
    45841 acgcctggaa gggttttaat aacatcttcc ataccgttgg ctgttggcag gttcgacgct
    45901 attacaggat tcaggcgtat caggttggtg cgtctttcgt ttttatctgc tacatcaatg
    45961 cttggtaata tatttacttc tttatggagt acaatatttt tttcggtaac cgatgcagga
    46021 ctcagggtaa ttgattcgga atatttctga taaccaataa aggtaatagt aatagtatac
    46081 tctttatttg caggtatttg cagggtaaaa aagccgtttt tatcggttgc cgttcctaag
    46141 ccgcccggtg aaacactaac attggcaaat tgcacagcat ggttttcatc gtcaattaca
    46201 taaccaacca acgtagcata ttggccatag ctaaaaagcg aaataataaa caataacgaa
    46261 aaaaaacaag cgcgtttcat caaaatatcc tgttaggtat ataacaacat cacgaaagaa
    46321 aaattgtgtt tttcattatc aggccttatt tatttcagga tattcaatcc gtgtatggta
    46381 aatattcatt aatttttcga taaatatttc ttttgtttca gagatattcc ggaatgttag
    46441 gttcactttt tcgaactgac cttcggcaac ctgcccgtca atgattttat ttacaagagc
    46501 tgcaatagtt tctcgcgtgt acgatttcaa cgaccttgat gatgcttcca cagcatctgc
    46561 catcatgatg actgcatttt cgattgtctc aggttttggg cccggatagg tgaatttggc
    46621 aggatcgata ttgattgcgt tatgctgatt gatatagctg ttataaaaat atttggtcat
    46681 tgttgttcca tgatgcgtgg caataaatga aacaattgtt gaaggtatgc gatacttttt
    46741 agccagctca aggcctttat tcacatgatt gataatgatt gatgcactct catcgaaagt
    46801 aaggttatcg tgaatattca cgccatgttg gttttcgata aagtattcag gaatctcggt
    46861 tttaccaata tcatgatata aagcacccgt gcgaacgagt aatgcactgc caccaatttt
    46921 ccttattaca ctttcggcta ggtttgctac ctgcatcgag tgctggaatg tgccgggagc
    46981 cttttcggaa agggttctga gggccggatt atttgtatcg gataactcaa gcaaagttac
    47041 atcggagata aagccaaaaa tacgctcata taaatacatg agcgggaata gtgtcaacag
    47101 aaagaatgca ttcccgccaa accacgcata ggtttcccag tttatttcgc tgaacccaat
    47161 gccattaata attgtaaacc cggaatatgc cacaacatac gaaaccaata ccagtaatac
    47221 cgagacaaac aggtgaaacc tcctttgtat atgccgtaaa ctaaacaaaa ccaccatacc
    47281 ggcagttatc tgaataaata caaactcgaa gctattcggt gcaaaaaagc caatcagcag
    47341 catcagcatc cagaagctta ccaaagcaat tctgttgtta aagaaagtat aaataa
//
LOCUS       DAOOQP010000009        45838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_882_length_45838_cov_5.900574, whole genome shotgun sequence.
ACCESSION   DAOOQP010000009 DAOOQP010000000
VERSION     DAOOQP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 45838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_45838_cov_5.900574"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLK75_01855"
     CDS             <1..495
                     /locus_tag="PLK75_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY38020.1"
                     /translation="CLTTQYPDNVHVIELKRKVEAKMRVANGNPAPEIALPDTNGSIM
                     KLSDLKGNIVLIDFWASWCKPCRQESPIMVALYEKYNKSGLVILGVSLDKDLNSWLDA
                     IQKDKLTWYHVSDLKGWDSVVCDDYSIGGIPYTAIIDKDGKILATGLRGETLEKKLEE
                     IFGF"
     gene            complement(602..1657)
                     /locus_tag="PLK75_01860"
     CDS             complement(602..1657)
                     /locus_tag="PLK75_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOY38021.1"
                     /translation="MSKIDPKFGKQLKSYGAFDYNKCYNCGTCTAVCSLADEKDAFPR
                     SMVRSSAVGNSDEISASLKPWLCYYCGECSEHCPQTANPGELMMSLRRWLMGKYDWTG
                     LAGMFYRSAAATIIAMIVVLAGVIGFAAIQDFDTESIMHFGHYFEMFAIGGVLLVILM
                     PNMIRMWYFTVIKPGKKIPFKIYLQSLGDFFLHMFTQKRALGCDDNKGRWFMHFILVL
                     GYMALLMTTVFLDWFGTEYLLVNIFGYAVSITVFTITFLFVNARIRKKKELSKHSQPS
                     DWFFVIWLFLMGFTAFAVRLFIDLGLIESNLWLYLFHLTILAQWALIIVPFGKWTHFL
                     YRSFAMYFHKLINAKTA"
     gene            complement(1650..1934)
                     /locus_tag="PLK75_01865"
     CDS             complement(1650..1934)
                     /locus_tag="PLK75_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38022.1"
                     /translation="MITENKQTYKILRETHKVSDKVKDNLKQFTRDKKQILDQLKEGE
                     KSIAELCTATQMKKDDVVFLLMTLLKYGFVKISRVDDMDEYYYYKLKNNE"
     gene            complement(1947..3653)
                     /locus_tag="PLK75_01870"
     CDS             complement(1947..3653)
                     /locus_tag="PLK75_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY38023.1"
                     /translation="MKKKLGIYICACGGNISDYVDIEKVKEAIKDEDAVILAKTTMFA
                     CGDTNQKDMDADIKANDLSGVVVASCSPKLHLTTFRNVSERAGLNPYTYVHANIREQA
                     SWAHSDDKTGATEKAIQIVKAAIAKARLAEALNPITIKSERSVAVVGAGVAGMKAALS
                     LAGMNDKVYLIEKSKEIGGKLKTMDVISPEKQSGSDLLKQLEAEIANNSRIELFTETM
                     LAESSGSIGNFTLKLNSPAGNREVTVGSVLLATGFDTYTPEEGEFGYHSHSNVITLPE
                     FKAILQNSSGKQLVVNDKEINSVAFIYCVGSRQVKGDNKHCSRFCCTAAMQTSLDATA
                     KFSNLQCYHFTRGVRSYGKQELIYKATNDKGDIYVQFLDKKPPVVEASGDKLSVKAVD
                     LLLEKREHEAEVDLVVLVTGMVPDKDETIGNIFKLPKGRDHFFNEIHMKLRPVETVID
                     GVTIAGACQGPKNVAESVNSAIAAATKAHSIIAKGELQLEPIVAYVDKETCNWCNACT
                     NACPFSAISTIDFNGKKVAEISKSNCKGCGMCLPVCPSDSIELISFTNKEMESMIDVL
                     AQ"
     gene            complement(3670..4965)
                     /locus_tag="PLK75_01875"
     CDS             complement(3670..4965)
                     /locus_tag="PLK75_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOY38024.1"
                     /translation="MQKEPEILNFDALVVGGGIAGGETALNLANNGYKVALVEKELSI
                     GGKMILLSKVFPTLDCAACITTPKVSEISRHPNISIFTETDVLSIEKLGEKNFEARVT
                     RRPRYVVEADCTACQLCEEACPVVVRDEYQYNLVGRKAAFIPFSICSPKVAAIDIDNC
                     TLCGACEKVCPPGCIDFTQKPREYVLKAKTTIVATGFKLFDPLKISRYSYGSAKNVIT
                     SMQMERQLAPTRPFHTILRPGDGKVPDKLAYVLCAGSRDDSVGNPICSQICCMYSIKQ
                     AQLLMGALPMADITIYYIDIRAFGKGYDEFYEQAVSMGVRFVKGKIGKITEKPNGNLS
                     LRYEDIATGKLKEAEHDMVVLSVGVIANAEAGNLFEGKKLELDVLNYVAQPSPLLSPS
                     KTNIEGVFVAGTATGPKDIPDSILSAGAAAGEAISYLNQ"
     gene            complement(4969..5364)
                     /locus_tag="PLK75_01880"
     CDS             complement(4969..5364)
                     /locus_tag="PLK75_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOY38025.1"
                     /translation="MAKKSAKILVFSTEKISDPAIDMAGLLKLHYPPQVYTIAMRCSS
                     SIKPKWIMRAFDKGFDGVFIAADGSDCAYGESCTEKTGHLVEQTHNLMKEQNIDPARL
                     RMAAICSVCSESFVKQVKSFNEYLLKSQE"
     gene            complement(5376..5633)
                     /locus_tag="PLK75_01885"
     CDS             complement(5376..5633)
                     /locus_tag="PLK75_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOY38026.1"
                     /translation="MTNEELKNLVVAKTVDARGTACPGPLLEAKKALGTIEEGEIMEI
                     LSADEGTRVDIPKWCNKQGHDYLGTLEENGYYKIYMRKNEI"
     gene            complement(5789..6355)
                     /locus_tag="PLK75_01890"
     CDS             complement(5789..6355)
                     /locus_tag="PLK75_01890"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOY38027.1"
                     /translation="MNEELQQQLSQMQAQLDKMGKKLAKLENNRKDQLSIAIVSGDLD
                     KIMAGMIISLAAAAMDTKVKIFFSFWALSALRDKNKSAKGKDFISKMFGIMLPKGKNK
                     LKLSNMNMAGMGPMMIKYLMKKHNVLSLDEMFKQAGELGIEIIVCEMSMGLMGFKKEE
                     FIDYPHLTYAGAATFVTDAGESAAQLFI"
     gene            complement(6580..7137)
                     /locus_tag="PLK75_01895"
     CDS             complement(6580..7137)
                     /locus_tag="PLK75_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY38028.1"
                     /translation="MKSKLLLITIALVAITIKAQSQASGTFTDSRDGKTYKTITIGSQ
                     TWMAENLNYITEAVAFCYDDSFPNCDKYGRLYNWSTAKNACPNGWHLPTKNEFDTLIS
                     HVGNYGAKGYKALKVGGRSGFDGLLGGYCTELYEYNHIEYCGYWWLSSESNEEKSWYF
                     YIMHGSATRYFTSKNDFLSVRCIKD"
     gene            complement(7208..7669)
                     /locus_tag="PLK75_01900"
     CDS             complement(7208..7669)
                     /locus_tag="PLK75_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38029.1"
                     /translation="MKKMILLLAVAGILISCNQNPSETSSIQKAPIDSLIENWQNAWN
                     NNDSAAIRNLFADEVVLIDDELVVLNTSDLSKNWIGPFHRYVKNMKVEKLQEWSSIDR
                     AGYTGNYGLDIVVNDSVIGHPHGVFTINWVKTSEGKWQITTANINAFSDVQ"
     gene            complement(7740..9077)
                     /locus_tag="PLK75_01905"
     CDS             complement(7740..9077)
                     /locus_tag="PLK75_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY38030.1"
                     /translation="MKTIRNHIIIVASIIAFAMFYSCEKVDIGDKPAPWILLENWSYS
                     QHKYIVSEDTVVKPYSNTDQYWNGEIMFLVAAPNGIKEISIKKNGVELPIIALNPNTV
                     NYSSGQIISLHFETDLDSAIVDIMVIDLAGLTTSKRVSVFYSVSGFRPGKPIANFIVY
                     DTEVYTGEEVYFANTTINGASYFWDFGDNTTSTAKNPYHVYETPGNYTVTLTATNNYG
                     DSTVTKTDYIHVSLNPEWAMDADGNIYHSVKIGDQYWLVENLRTTKYNNGDPIPNVID
                     NSTWYSLNTGAFCYYNNYAANADTYGVMYNWYAVSDPRGIAPEGWHVASYDEWTTMQN
                     YLISHYYNYDNTNFENKIAKAMASTTLWDNSTVPGTPGNTDYSSFRNKSGFTAYPGGM
                     RNADLGGVFDGMGMMTWWRTSDCYSFAIFNEVVSLYSTNSAYVNGEYIRCVKD"
     gene            complement(9096..10073)
                     /locus_tag="PLK75_01910"
     CDS             complement(9096..10073)
                     /locus_tag="PLK75_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38031.1"
                     /translation="MKKIIFVIISVIAWAISLNAQDQRQHFGLVPPPTNRNFSFVVAA
                     SWFTMAGSYGETLEFSLHYFKPRIALDVYYERAIQDWFRGALSDENEYQLLDNFFSRL
                     EFDGALTFSSKVSRKGSFFYRGRDYEGGNAYDVYSYGEGNAATYNQIRFGVTNMQLAF
                     PTDGLDTVPVCHYPYPTQLQSYYYSYSTQNILSSSRYTMFHLGIGTTKVFNVGSPGTR
                     EGTDRIGMRKMYIDIVYAPFINYRFLTEKGNEVFPGYIPTQYIQKPGFSIGIEMNRLN
                     AFSGYWMFEIGMLPGIRPYHESMGSSFLLYFSLKAGLETGWPLFNRNYE"
     gene            complement(10099..10704)
                     /locus_tag="PLK75_01915"
     CDS             complement(10099..10704)
                     /locus_tag="PLK75_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY38032.1"
                     /translation="MKKAILLLALAMSVSFGFCQEFGSFTDTRDGKVYKTVIINHVTW
                     LAENLAFAPDSGNYFAYEYKDKHVKKFGYLYDWETAMTSCPEGWHLPDAIEWNLLIMN
                     NGGANAGAKLKASGAFYYDENIEYSYNESGLGILPAGFYNSEKDEFGNLKWTSILWSS
                     TTVFEDNEMAYSYCFTFSTNLVEQYTSWKTNGYSVRCIKNY"
     gene            complement(10802..11632)
                     /locus_tag="PLK75_01920"
     CDS             complement(10802..11632)
                     /locus_tag="PLK75_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOY38033.1"
                     /translation="MKKIYFILFISCLAASLTKAQTVTTDSIESVILNRKVATTVILP
                     AGYDTTQVYPVMYLLHWWGGNNDSFLETNLPSELKNRQIIVVTPDAGTNWYVNSFSNP
                     TAKYESFMTEELFTYIDKKYKTDPSWQGIGGFSMGGYGALLVGLKHPGRFTFIADMCG
                     AINAPFYDIPLTPESPLNFIMSSVREAFGDENSYQSRGTDVFEIVSNMKKGSNQFVYM
                     AMATKDEFDFIVPQHQKLKKELDKKKIKYHYIEIEGGHFDGIVFSTCLPEMLDQFTKK
                     "
     gene            complement(11637..12260)
                     /locus_tag="PLK75_01925"
     CDS             complement(11637..12260)
                     /locus_tag="PLK75_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY38034.1"
                     /translation="MISVSIVEDDHDLRKSVENIVGQAIDCLLVKGYANAEAFLHDLE
                     VNTPEVVIMDIELPGMNGISCVEAAKSKFPEIQFVMFTIFDDDESVFQSLCSGATGYL
                     LKNSRPEQIISAIAEIHSGGSPMSPQIARRVIEQFSLKTRISNEIHVLSQREREILDL
                     LSQGFRYKEIAAKVFISIETVRTHIRNIYEKLQVQSRTEAINKIYKH"
     gene            complement(12335..15334)
                     /locus_tag="PLK75_01930"
     CDS             complement(12335..15334)
                     /locus_tag="PLK75_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY38035.1"
                     /translation="MNRMKFNVLFIILIIKSVTLNAQQYQFWEFGADQGLKSNDVTKI
                     IQDSRGYLWATTMGNGIYRFNGNTFDNYTEKDGLGDNNTGSIVELPDGRLMILSLKCI
                     SIYDGLKFTNYTSKDGLALPGQWASTAFDKKGRLWIALWNADETRQLLYFENNRFHDI
                     TPVNPFLNAPESLLLSVVKNGDGDIIVNTLGNMYIVNDTVLNEYNPGVPAEVSENWLN
                     LFYETADHTQFFLSFDAKIQKLSIFTLQNGVITYLDMPAEVGYWFLSGVCEDHENNLW
                     VPSEFGLWRVSSNGEVKHFNEQNGLPIELVFWVTEDNENNIWLATRGLGLIKFMGEKF
                     ICLNEKDGLKSSKVYFNFKDSQGNIWFSTPTDQVCKFNGSEILYIDAPDNRETLGFFE
                     TNEHQVIVLTYQGLFDANSSNGYQLNNMFDLESDVIPYIAYNDGKYIWFSANGSSLIR
                     FDGKTSAVVSAEEIGAEAGTILSITSDNIGNVWFAANAGYIKWDGKMYSYTTYPKNSI
                     SPDDIAVDKWNRVWIALYGGLLLVDTHGQRLLTTEDGLSSGYYVSLGFDNKGNMYAGY
                     NQGFDLISFTQDGSVSSIKYYGKGDGFIGSSLGAQNINTDKKGNLYFATTQGLIRLDP
                     SQLTPNTKPPIVNIRKIKLFYKDVNWESSEFRKFCDSVSPWFRLPVNLCLPYDKNHVT
                     FEFEAICFTNPDAVKYQWMLQGLEKDWSPVSEQTKAEYANLEPGDYTFLVKACNNDGV
                     WNNQPLAYRFTIKPPFWQTWWFIALAIAAVVTILAVIIRFILYLKYRKKIEELNKLKE
                     IEKIRSALSKDIHDGAGASLSKISLLSESLKSEIKDSQSSLNKLTQISLLSRRVIDDF
                     REIIWSTNPKYDNISSLLAYIRNYCNNFFEDSGIECDINFPSVHEEINISPLLRQNFF
                     LILKEALHNIVKHASATSCFVEISLTENQIRLQITDNGIGFDLEQAGVFSTGIGNMNK
                     RATACGGSITFDSAKNKGTTVTLIVPL"
     gene            15537..16214
                     /locus_tag="PLK75_01935"
     CDS             15537..16214
                     /locus_tag="PLK75_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY38036.1"
                     /translation="MNHNCSFCEHKSAAAHKLNNDELSALSANCLTVKFKKGDNIIRQ
                     GTYSTNVAYLKSGLVKIHMEGPYHTQVVRIVKPRNYLGLPTTFGDKINQYSVTAIEFS
                     EVCFIDITTFRDLLNNSPAFSNQILIELCRGELESYQKCVNRTQKQVRAKVADVLLDF
                     CENIYKSNSFQLNITQEDIGNLVDATRESVSRIWNEFIKDDIIRVDGKTIEIVNIANL
                     KNISRKG"
     gene            16221..17459
                     /locus_tag="PLK75_01940"
     CDS             16221..17459
                     /locus_tag="PLK75_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38037.1"
                     /translation="MTVHFTSGIRSKWMQLTVYDKKHPVVLILLVAIPVLLWFGYLNI
                     MNWTDGSISNGIPGLKFWLDSERYIDGATSIAHGMPLQGREIQFTGYMLLIALVQSLH
                     LPLSVMAVIQIAIALLAAVALYDSCRLLSGSRIAGLFAAALFLCNPFIARWHIYIMTE
                     SLYTSLIVLCFWSLLRLLIRKKPVDYIVSAIFLISAMFVRPNGWILLPVFFIVYLYSF
                     NLKKWVFIISVFATIVAFTLTMAKVPVFRSSVQITTPVENLQKGITVWGHGELSLKMP
                     QDKDIDKTQWTSGWKYIARHPWVSFKLAAARAGYSLIHIRPYHSTAYTWHVALWVFPA
                     YLLALIAFIGYRRKTAVYVALMIILGHLLVVALSYAEHDSRFDIYILPVFYIFAGLGL
                     LELLKKVSSLISPKVDQAKL"
     gene            17553..18422
                     /locus_tag="PLK75_01945"
     CDS             17553..18422
                     /locus_tag="PLK75_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38038.1"
                     /translation="MRKIFDRLIVFVGSLEIKKDQLSFDLSILFSIVFYQILTKVAGK
                     TVTQMLSPAATLFLCMFFLLILSMSCGYLHMLYARQRRIWGYLTILVTVFLMMFPLTV
                     MSRIADAGDLGMIATFLTGTGFVMGRIIAPDKMALTIKRYVLLLLLVPASVGLIFWMI
                     SFTGDEGYKGVLICVLGFAAVAGLGFIIHKLFSRSETALNNFFLSPNAKGIAKFLIYF
                     FWCVAAFAICTWQEVLTDAPQDSGFYRIFILTLSGIIPYRILWAFIPPRKLATTIIAT
                     VVLVINMLFVYMG"
     gene            complement(18460..19155)
                     /locus_tag="PLK75_01950"
     CDS             complement(18460..19155)
                     /locus_tag="PLK75_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOY38039.1"
                     /translation="MKKKKLVVLTGAGISAESGISTFRDSGGLWENHKIEEVASPEGW
                     KKNKALVLDFYNQRRKNVARAQPNEAHKILVQLEKIYNVQIITQNIDDLHERAGSTWV
                     LHLHGEIKKARSTANPEIVVDINGTELNVGDLCPLGSQLRPHIVWFGEAVPLIEDAVE
                     IVRTADIFLVVGTGLQVYPAASLIEYIPEHCLRFLIDPNNVFIPSFYRFKVIKENATI
                     GMQLFTSLPDIKL"
     gene            19332..19796
                     /locus_tag="PLK75_01955"
     CDS             19332..19796
                     /locus_tag="PLK75_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOY38040.1"
                     /translation="MNMFLEGILAISGQPGLYKQVSQSKNSIIVESIETGKRFPTFSA
                     AKISALQDVAIYTSDKEVHLAEVFSNAYNAMEQFPNISPKSADKELYAYFEVILPDYD
                     REKVYLSDIRKVVSWFTLLHKANIITRENLDAYFEEVKNAEAAENEAEPAEE"
     gene            complement(19813..20577)
                     /locus_tag="PLK75_01960"
     CDS             complement(19813..20577)
                     /locus_tag="PLK75_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOY38041.1"
                     /translation="MKKLMRFLVRNVPRPVLIQFSYLFAAMVAPFYKGKNYECPICGN
                     RFRKMLPYGNKGADNRLCPRCLSLERHRLLWIYLRDYTDMFNKTYTVLHIAPEQSLRR
                     RFKKMEKLSYTTADMVSPIAELHFDVMHIPLPDNSYDVVFCNHVLEHVENDIDAMKEI
                     YRILKPKGWAIMQVPINQSLETTFEDKNITTPAEREKVFGQYDHVRWHGKDYPDRLKS
                     AGFRVEIFDPVTALGQDVMGLYRLDNDEKLFIAFKY"
     gene            complement(20574..23819)
                     /locus_tag="PLK75_01965"
     CDS             complement(20574..23819)
                     /locus_tag="PLK75_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOY38042.1"
                     /translation="MSLLHVYSASAGAGKTHRLTGDCLRLLVNQPDAYRHILAVTFTN
                     KASEEMKERILNELYNLSSGNNSDFLAELCHGNPPAQEKIVRKKAEAVLNMILHDYGR
                     FLYMTIDSFFQRILRNFMREMNLNSNFELELDSGSIVNQVVDQMLDDTANDEDLLEWV
                     LEVVTRKMDVSGSWDFRSELVELFNELFKEKFAYIEKIYRLKKLCRNDVKKLVSLVTS
                     IENKHENFIKKIGSEAEEILIKHNLTPENFHQKNNGPAGFLLKLRNGFKEPGVYFLNG
                     IDNEDKFIGKAPNPEAVLRKVYPLLNALSKKYLDWCNTSRKEFFTAKQIRNNIYLLGL
                     IVDGKRRLADYLNENDRFFIGDTPLFLAEMSENNDAPFIFEKAGIMLKHYLIDEFQDT
                     SIKQWEAFLPLLKECISDGNNYGLVVGDVKQSIFRWRNGSWKILAEDINTQFSVKREN
                     LSANWRSLENIVTFNNTIFGKVPEMIQNHFNSQINTDEKCSNHKLLTSITSNYAEVSQ
                     NVKAGPGGHVRVKFFTDEPKQGTDWELSSMQETIERIDSLLLQNFNPGNIAILVRTHT
                     EAKRAAALILEHQSKEKPDERLRYPVVSSESLVLSSNPAIIVITGIMRYILEPGNTIA
                     LYTACLNYLIHFTGEEDKITNISAHSGIQTLTDVFPDDFGTLIDHSAGTDLIALAESI
                     IAMCKLNEFSDCIPFIDSFVDCAADFSRSKGTGLSGFLEWWESDGSRKSLQMPDSDNA
                     LRILTIHQSKGLGFDVVFLPFCEFYLEPKEHYPIIWADTSGHMYDAVPFIPLKYNAEL
                     GNTDFCNEYFEEKLNCYLDKLNLAYVAFTRAKKAMYIHCPSRRNDNIESSVSGFLQTL
                     LQGKFSSDDGFSNDLRKHYDVSTNCFEVGHTQAIEESSKRCSIEAIHPVVKNKKQAVT
                     HGDYTRLLEAEENLPVEQGILYHKIFENIRNTGDIEKAINQVHAKNLLPASEKPALTK
                     KIAEIISQPKISDWFTGYTDLHRENWILAPDGSIHKPDRVMSFDDHIAVVDYKFGNKR
                     SAEHINQVFDYMQLISLMGKTCVKGYVWYVFGRQLIEINMKKEITLTELIA"
     gene            complement(23819..24115)
                     /locus_tag="PLK75_01970"
     CDS             complement(23819..24115)
                     /locus_tag="PLK75_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38043.1"
                     /translation="MKFILIPLFVAIVLLTSCGVKESCEVNHTGEICVSNYSGRITEV
                     YVNDVFAFTLANDASNCVAKPVGEYSVRFISLADERIIPVTVTECETNNLTVSF"
     gene            24202..24912
                     /locus_tag="PLK75_01975"
     CDS             24202..24912
                     /locus_tag="PLK75_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HOY38044.1"
                     /translation="MKYLLLSILLLPVFLLNSCDVDDPPDDPDDNDTINTTAIVNGSF
                     ELWTMESQNNVEFETPGGGYWASLNNLVFLGGPLVATKTTNSHTGSYALHLEAKAWGD
                     DWVIPGIIAAGIFDSNQTIGENLVMGRAITVEPTSISMYYRYLPADNDTAVIYSSLTK
                     YNQNLQRRDTIAEAYLTIVDVANEYTHLVLPFEYRSALHDHDTINIIFLTSISGDKMK
                     AHEGSILEVDDIELIVTE"
     gene            24912..25655
                     /locus_tag="PLK75_01980"
     CDS             24912..25655
                     /locus_tag="PLK75_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY38045.1"
                     /translation="MKYILTYLIFFIISVSVFSQPVVILAGGNFGGAIPDTKVGNSTG
                     SGGIGFIVGAGIDFKAGDRFYISPSLSYVARRFSYESHQTKDTVVEIEFQGSTAHIPT
                     YYTANVFGNVFTHNIDLSVNVNYLIRERFLLNAGASGKYAFAGNDNTHIVVTIGEGGI
                     IDDIITDQNNWNKINRFDVGFDLGASFLLSDDFTLLVSGERSLTRFYNAGYMKTESGG
                     PVSFYYTAMIIKLIYSVNLHNPKFPASSI"
     gene            complement(25670..26242)
                     /locus_tag="PLK75_01985"
     CDS             complement(25670..26242)
                     /locus_tag="PLK75_01985"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOY38046.1"
                     /translation="MLNIAIFGPPGAGKGTQAQMLVKKYHLEYISTGDILRKEIANKT
                     PLGLEAQQIIEKGLLVSDDVMARIIEAFIAKNIHCEGMLFDGYPRTSAQATILENLLQ
                     KNKMKLAGIIGLEVEQQILIERMLKRAEIEKRSDDTPEAIEKRLQEYHNKTFPVIDFY
                     KIKHLYYPVDGMGTVDEVHLRIVNSIEMMQ"
     gene            complement(26253..26807)
                     /gene="hpt"
                     /locus_tag="PLK75_01990"
     CDS             complement(26253..26807)
                     /gene="hpt"
                     /locus_tag="PLK75_01990"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOY38047.1"
                     /translation="MTYKNQKSIKLHDKEFVAFIDNLKMEKAIKELADRMSTELSEEQ
                     IVFLCILNGSFMFAGELFKHFENVCHISFVKLSSYSGTATTGDVKEIIGLNESIEGKT
                     VVVVEDIVDTGETIEKLYHTLMKKQPKQVKIATMLFKPDAYRKDIPIDYIAINIPNKF
                     VVGFGLDYNGYGRNLKHLYQIIEP"
     gene            26859..27368
                     /gene="purE"
                     /locus_tag="PLK75_01995"
     CDS             26859..27368
                     /gene="purE"
                     /locus_tag="PLK75_01995"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOY38048.1"
                     /translation="MKPIVSIIMGSTSDWGVMQKAASALNDFKIPFEINALSAHRTPF
                     EVEKFATTAKERGVKVIIAGAGMAAHLPGVVAAYTTVPVIGVPIKSSFDGLDALLAIV
                     QMPPGIPVATVAVDGAYNAAVLALQMLAITEENFAKTLAEFKNNLKDKIVAANNDLAK
                     FTFDYRVTE"
     gene            27365..28180
                     /locus_tag="PLK75_02000"
     CDS             27365..28180
                     /locus_tag="PLK75_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38049.1"
                     /translation="MKKIISLISFFILATGAYADGDLLTAGARYAGMAGSSVVLPDTW
                     SVFGNQAGLAYLQNPSVGLSYENRFLLPETSYGALAFQMPLLAGNIGLGINHFGLTEY
                     HENKFALSYAQQLFRRISMGVQFDYIFLHQPEDYGNLHAFSFELGLIAKPNDKIVIGI
                     HAANPMPVKILNTENQYLPTVLKVGVGYVFSKSVITTFEAEQNLQLEKPVFRLGCEYN
                     SERHYSIRAGIATNPVLFSLGFGYNLKGLQLDIAYSYHQVLGSTPHISVGYAF"
     gene            28170..30248
                     /locus_tag="PLK75_02005"
     CDS             28170..30248
                     /locus_tag="PLK75_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOY38050.1"
                     /translation="MRFKIVAILLLIMPVAMCSFAQNNEMQSLSDIIEDIAEYSDEDL
                     DYSSLYENLFIFYKDPLDLNKTDIEELEKLQFLTFFQVRSILNYRDKFDGFKSIYELQ
                     FVDGIDQQTLEYLLYFVTVAEKPGAKEVDMSKSLKYGRHVLFVRTQFTLQEKAGYITV
                     PDSVLAVDPDKSRYLGSPYKLYTRYRFNYKDKMYWGITAEKDAGEEFFRGSQPYGFDY
                     YSAHFQINKLGPVRTAIIGDYQVEFGQGLTLWSGMGFGKSSSPNNVIKKGRGITRYGS
                     TNENEFLRGEAAAFKIGNFTITEFFSYKSIDASTDADSTYDDDEIVSSFLNTGYHRTP
                     NEIATKDGIKELIAGGNVNYRGKWLAVGATVAAVKYSAPFTGNGRLYNLFSFSGTGMM
                     NAGINYVSNFGKFSIFGETAISDNLALATLNGMSVIMAPEVSLSVLHRYYEPDYHAIY
                     ASPFSEGNKPSNESGFYIGSTIYPRKRWRIDLYADLWKYPWLRYGVDAPSDGQEYFAQ
                     INYSPYRNVDMFFRAKYETKQENNPITEYGVQDIIDYSSLKLRYQLSVSPGYGFQLKT
                     RLEWNRYQQDVAEPENGFLLCQDVGYTFRKFPLDVTARVAVFNSETYNARFYVWEPDV
                     LYAFSIPVYYDKGNQLLLLLKYSFGEHLDCWFKIANTFYWNKTSVSSGLEEIEGNKKT
                     EVKFQIRYNF"
     gene            30250..30729
                     /locus_tag="PLK75_02010"
     CDS             30250..30729
                     /locus_tag="PLK75_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38051.1"
                     /translation="MLIYSAKEHEKFLREIVIEPPGKMLEAGFTYHAVILMAQSIEIF
                     GAYFDNKPFRSIGQSLNRFALGIDNLFPYAYHRANARNQLYKQLRALFVHSFLPGTNL
                     QIKEQGRTEEHLEQKNATIIIIPAVLHNDVLHAGIKLIKKLNDNQINPKKVSTSLFS"
     gene            complement(30722..31519)
                     /locus_tag="PLK75_02015"
     CDS             complement(30722..31519)
                     /locus_tag="PLK75_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY38052.1"
                     /translation="MEKCILVPWDFTEISNYALEHAYAIGKSSKRPIYLVHVASKSSK
                     VPELLKQLQEIAEQFAAGKDIKVNTAVITGNLFKAIYKFGLENNAYLGVMGTHGIKSL
                     DKAIKLVKKFVKIPFILVQSPVIFGEYDRICIPMDATKKSRAKFQWVRYLSTLFESKA
                     FVIYPTVSDTFRIKTLNNNLRFAEKIFEKDLIDYEMRRVDVGSHYPDAIYDYIREVEA
                     DLVLMMTNKYREYIKNLKKHENKDLYKKIPVMCINIRTDIQKLGGFN"
     gene            complement(31614..33332)
                     /gene="recJ"
                     /locus_tag="PLK75_02020"
     CDS             complement(31614..33332)
                     /gene="recJ"
                     /locus_tag="PLK75_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOY38053.1"
                     /translation="MDKKWVLKEKAAEEKVLRLMKELNVEATIAELLIQRGIETYDAA
                     RVFFRPELSDLHDPFLMKDMDKAVERLNEAIKKNEKILIYGDYDVDGTTAVALVYSYL
                     RKYHNRIGYYVPDRYSEGYGVSIQGIDHAAEENYNLIITLDCGIKATEKARYAQGKGI
                     DVIICDHHNPGEELPCAVAVLDPKRSDCPYPYKELSGCGVGFKFMQAYIIHNGLNIQE
                     LYSFLDLVVVSIGSDIVPITGENRVLAYYGLQKLNSDPCKGLKSIINISGLNNKHIQI
                     HDIVFKLGPRINAAGRMESGSMAVDLLVAELDHFADKIGNDINNFNTNRKELDQKITE
                     EALRMIASDPDSERKKTTVVFSKDWHKGVIGIVASRLTETYYRPTIVMTLSNGMITGS
                     ARSVTGYNLYSAVESCSDLLENFGGHMYAAGLSLKPENFEEFRDRFENYVATTIKADQ
                     LIPTIEIDAELKIKDITPKFYRILNQFAPFGPNNMMPLFLSTHVYDNGSLKKVGKQEE
                     HLKVKVVQELGANLCIEGIGFSLGEKYSDVFDFKPFDVCYHIFENHFMGQTFLQLMIK
                     DIRKHE"
     gene            33464..35191
                     /gene="lysS"
                     /locus_tag="PLK75_02025"
     CDS             33464..35191
                     /gene="lysS"
                     /locus_tag="PLK75_02025"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOY38054.1"
                     /translation="MNIIGLSEQEIVRRNALNELRSLNINPYPAAEYKVNISAGTILS
                     TFSDENPEELRDVSLAGRIMSRRIMGSASFAELQDDTGRIQLYFNRDAICPSEDKTMY
                     NTVFKRLLDIGDIIGVKGYVFRTKMGEISVHVEEFTLLCKSVMPLPIVKEKDGQVFDA
                     FTDPDLRYRNRSVDMIVNPHVKEVFRKRTLLTNTMRNFLNSFGFLEVETPVLQPVYGG
                     AAARPFKTHHNTLDQTLYLRIANELYLKRLIVGGFDGVYEFSKDFRNEGMDRFHNPEF
                     TQMELYVAYKDYEWMMNLVEEMVENIAMALHGTPELTVGDNTISFKRPWKRYTMFEAI
                     QHFTGVDISKMDENELRKTAISLGAEINGSMGKGKLVDAIFGECCESKLIQPTFITDY
                     PVEMSPLAKKHRSKEGLVERFEAICNGKEICNAYSELNDPIDQRERFEQQLLLAKRGD
                     EEAMMLDEDFLKAIEVGMPPTAGLGIGIDRLCMIMTNSVSIQDVIFFPQMRPERKVVV
                     DDTDAFIALGIPEEWIQIVQKSGFASVDLLKQANPNKLHAQLCGWNKKHKLGLKNPTP
                     DEVKAWISK"
     gene            35255..36526
                     /locus_tag="PLK75_02030"
     CDS             35255..36526
                     /locus_tag="PLK75_02030"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOY38055.1"
                     /translation="MKIEYRFLEPFVSTEQIREYTQRAVVALQTIKKRNGAGNDFLGW
                     LDLPMQYNPSFAGEIASVVNDLKHLETVVVVGIGGSYLGARAVIDALAKPFEKQQPEI
                     IYAGNHLSQPYHEALLRYLETRSFGIIVISKSGTTTEPAIAFRLLRSLLISKEGLEKA
                     SRHIVAITDKSKGALRMMADREHYKTFVIEDNVGGRYSVLSPVGLIPIALAGISTDEL
                     MAGASAATTDTMLENVVNPAVQYAAIRNCLLDSGKTTEIMVTYEPCLQYIAEWWKQLY
                     GESEGKQHKGLFPASVQFTTDLHSMGQYIQDGKRMMFETVLSVAKTQGSLVIPHDDND
                     FDKLNYLADKSLSYVNDIARMATAQAHTDGGVPVLCIELPDLNAYHVGYLLYMLEVAC
                     AISGYMLGVNPFDQPGVEAYKKNMFRMLGKE"
     gene            36663..38666
                     /locus_tag="PLK75_02035"
     CDS             36663..38666
                     /locus_tag="PLK75_02035"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38056.1"
                     /translation="MKNRILLMLLFVCSFYSILAQQSCSEAVEISFLEYSTCGQIAIT
                     SADLADAAPSEDYPSPECGNFSTTTNDLWYKIVTPLDVNELAFHVINPGMLSDPYTFF
                     RPALAVYSGSPGNLELLQCFYDEGGPFSNGEIRFSLITNLTPGQTIYLRVWDMNNNPF
                     PFFIAASVRTEIQEYNCNTPEILSDGGCNILAPEGTIDAPEKCGWNTSDNTCYFSFVV
                     NPSDPQPVTIGANYVFCFENGGTYEYPDNTVLQMAIYSWNGYDCSWLGGSPQSMNPND
                     STYWGCNSGTGVVSISALLDPGLYVLALDGYRGLSGTSLCTFEFSSNIANQFQCHNDT
                     IICYFSPPVNLGENLPQGGVWEPSQFVNDGIFNPAEPGDGIWEIIYSLGGYTCSSTIS
                     VASYPVNISDIEICLVSVDENNKNRILWEKPDTDSLQAFHIYKDNGVAYDLIGSVPYE
                     HESYFVDYNSEPQTQSFRYKIAGYSVCEFESELSDYHQTILLNMVEESGTWHLSWTPY
                     IGAEVGSINVLRGTNPSDLITIASFDAETTEFTDTDPPAGFVYYQIEVILTVHCNVEK
                     SVSVVRSNISTNDPDYFNTGIGMGVRDISNKICPNPAGNSFSLCTGSMVEDVFIFNIH
                     GEKVRDIRNYKNQIIDVSELEKGIYFVRIIGNEEVGMGKLIIE"
     gene            complement(38841..41264)
                     /locus_tag="PLK75_02040"
     CDS             complement(38841..41264)
                     /locus_tag="PLK75_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38057.1"
                     /translation="MKHVFTPASEISLFLILLICGNSFSQTTDGQPHIEPYPVPEIYY
                     TLNAPELPEKVLNYKLPYFPQQFYNQANVPSCGQASAIYYCMTYEFNRIKNQAADPSN
                     TFSTLYTYFFLNYGNNYFGASSFDSWDIVMSQGHPVLSDFPEPFPHPGSPNGSRIPAW
                     MSGYEKYYRAMKNRISGYYSLDVSTDEGLRLLQHYLHDHLRQESSGGIAINYGAPDYG
                     PSFQDTALQSTGFVNISECIGNTYSHSMTVVGYYPNTFYDFNQDGLITDTLDLNNDGI
                     VNFRDNEKIFWVMANSYVGSYRLYLYKYDMLADCHDGKVYFPVPDTTYQPELTAKIRM
                     SHSLRGLIKISIGISEDIESLQPEHIIDFPIFNFQGGLLPMQGDDYLTDADTLELGLD
                     LNPLLKYIQNPGISKIFIIIDNASHVNGQLKSFSIIRYDGDTLFEYAALQHDTLIAAK
                     EVFKLGFNVDLPSHYDSNLLNIQYSGGLQTEINTEFQTQLTATGGYPPYRFYLYREDE
                     YLQELIDAPYDTEPDENSVYLSESQVSAGWPVNFAGEIFDTISIGRQAEIYFSTEEPW
                     FHEAYPYEYQPQISYSDMEFCHYYPNSESFQRRISLSDSCLTIFQYKFREVYFKTLLY
                     KNGNIQFGYISNVTDWFHSSYVKTRCYTYYSKLLPRSCSSEIQSTLFTWNPYYGPFSL
                     EPTGLLTMQPSSAAGLYPVYIMVIDSSGNKAYKRIQVEALENSIPENNSAQNMRLFPN
                     PANNLLNIKLTDYENGYFSIFDLNGRCVMCGDLQPNINISELQDGVYILEIKTQTYTK
                     RTKFIKQNQ"
     gene            complement(41318..43438)
                     /locus_tag="PLK75_02045"
     CDS             complement(41318..43438)
                     /locus_tag="PLK75_02045"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOY38058.1"
                     /translation="MRALLLFIGSMLLLIPTSYGQWVWQNPTPTGESIYAMIFVDSHT
                     GYASGGAGHIIKTTNGGFSWYSIGSVGDDYIMSLSFLNENKGFAFGNEGNLYYTIDGG
                     NEWTTLETGFENQNFVAGHFFNSMLGVIACWNGKILRTTDGGNSWSVAFSTMDKNIWD
                     MQFVDQSTGYVCCDGGYVYKTTNGGANWTAKPTGSSEFLSSLFFTDANTGYACGFLGT
                     MMKTTDGGNNWTAQTVGEDINFTESYLRKCFFTDANHGWVGNDFGEIFITSDGGTTWE
                     LQDITNNQMINAICATSVGTLFVANMLGEIYRSNNSGASWILMTDKLTEEYCVLYNVE
                     FYDDNIGTAVGNDGNLLHTANGGNTWELDKFSDDLDFTDVEYLGENNIVISTNYGQVM
                     LSDDGGETWETVVLMNNFLITSIFFTNELIGYAAGSNGHVFKTTNGGHNWAEQNSATD
                     SYLQDIFFTNDTVGYAVGSYHTTIKTSDGGSTWQNMGVFEDNGSLYSVAFVNNDTGFV
                     CGYIAENFNSQTGEFTAVPVIKKTYDGGVNWQLVDGISSQSAWFNDIMFNEGDTGYVA
                     GQSGAFLMSVDAGETWETIPRFTYQDLNAIAETPVGSLIVVGHNTAILKNRYAPKMVH
                     IEEPVPTEKVNVYPNPANNLLSISLTDYENAYFSIFDLKGRCVMCGDLQPNITISELQ
                     DGVYILEIKTQTYTKRTKFIKQNQ"
     regulatory      43593..43799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            43918..45165
                     /locus_tag="PLK75_02050"
     CDS             43918..45165
                     /locus_tag="PLK75_02050"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOY38059.1"
                     /translation="MKKKVLFTALLLLSVGTNAVLHAQQNVDFEELTLDPESHWNGSD
                     GSGMFTTGGWNFINVYDDVYGSWMGWSYTNETDNTTYDWQNMYSSASGEGLFGSENYA
                     TAYVMSDWMNNNEPIPCVVQRSDNQPVEMFGTWICLNANASLYMAYDDFYSNQNHYLK
                     LIVNGHMSEVLPAMVTQEYILADYRFENDEYDFKYDNWHFISLMGLGSAYKLEFTLVS
                     DDMGEYGLVTPGYFCIDNFNSEYFLSYLPDMFVETPEIITIDEGQSVDLTALAGGGIQ
                     PYTFEWQYNESLSSTSGQTVTASPAETTTYYVTVSDVMPNSPGHTVEKSITVVVNHVG
                     IDSKQLNTVNVHPNPCSDVVTVSNVPAGSEVQIYDLQGRIVTSVIAQSSSVQIPVSQL
                     AAGVYQVISIAGEVRSIHKLVIR"
     gene            45169..>45838
                     /locus_tag="PLK75_02055"
     CDS             45169..>45838
                     /locus_tag="PLK75_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38060.1"
                     /translation="MLKVKNIILISFLISSSLLKAQVYDNYQNAVNVDDSRIVGWVSQ
                     CEVERGYINISDPEQTYTQGDVTSNRAFAGTEQDACGAANGLSTVSLGDGGSATVQFQ
                     NPIRDGEGYDFVVFENGFFNPPGETSAAFIELAFVEVSSDGTHFFRFQTLSEMPYLTQ
                     NNSFAAIDWTLFHGFAGVYAHPYGVAFDVADISDAPFLNKQRITYIRIVDVVGCIQPQ
                     YASYD"
BASE COUNT        12720 a         9432 c         9583 g        14103 t
ORIGIN      
        1 tgcctcacaa ctcaataccc cgataatgtt catgtaattg agctcaaacg taaagttgaa
       61 gcaaaaatgc gtgtagccaa cggaaatcct gcccccgaaa ttgcattgcc tgatacgaat
      121 ggcagcatta tgaaattatc agacctgaaa ggtaacattg tcctcatcga tttctgggca
      181 agctggtgta agccatgccg gcaagaaagc ccgattatgg ttgctcttta cgagaaatat
      241 aataaatcag gcctggtaat tttgggtgtt agtcttgata aagatttaaa cagttggctc
      301 gatgctattc aaaaggataa acttacatgg taccatgtga gcgaccttaa aggatgggat
      361 agtgttgttt gtgatgatta ttccattggc gggattccgt ataccgcgat aatcgataaa
      421 gatggtaaaa tattggctac aggtttacgt ggcgaaactc ttgagaaaaa actcgaagag
      481 atttttggct tttaatattg gcacactcaa ttatggtcaa aagaccttat tttctttgag
      541 taccctattg ccctagctta tcgaaggata gaaagttcaa agggcattac tattactcat
      601 ttcaggctgt ttttgcattt attagtttat gaaaatacat ggcaaacgac cggtacaaaa
      661 agtgtgtcca tttaccaaag ggaacaatga tgagcgccca ttgggcaagt attgtcaggt
      721 gaaacaaata cagccacaaa ttgctctcga taagccccag gtcgatgaac agacgtacag
      781 caaaggcggt aaaacccatc aggaaaagcc agattacaaa gaaccaatcg gatggttgcg
      841 agtgtttgct cagttctttc tttttacgga tacgtgcatt tacaaacaaa aaggtaatgg
      901 taaatacagt tatactcacc gcatatccaa aaatattcac cagcaaatat tcggttccaa
      961 accagtcgag aaatacagtg gtcatgagca gtgccatata acccaagacc agaataaagt
     1021 gcatgaacca gcgccctttg ttatcgtcgc aacctaaagc gcgtttttgg gtaaacatgt
     1081 gcaggaaaaa atcacccagc gattgcaggt aaattttaaa aggaattttc ttaccgggtt
     1141 ttatcaccgt gaaataccac atcctgatca tgttgggcat aagaattacc agcaatacgc
     1201 caccaatggc aaacatttca aagtaatgac caaagtgcat tatgctttcg gtgtcgaagt
     1261 cctgaatggc agcaaagcca attacaccgg ccagcacaac aatcatggca ataatggtag
     1321 ctgctgccga acggtagaac atgccggcca ggcctgtcca gtcgtatttt cccatcagcc
     1381 agcggcgcag cgacatcatg agttcgcccg ggttggcggt ttgcggacag tgctcggaac
     1441 attcaccaca gtaataacac agccagggtt tcagcgaggc cgaaatttcg tcagaattac
     1501 caacggcact gctgcgcacc atacttcgcg gaaaagcatc tttttcgtcg gctaatgaac
     1561 aaactgccgt gcaagtgccg caattgtagc atttattata atcgaatgct ccgtaacttt
     1621 tcagttgttt tccgaatttg gggtctatct tactcattgt tcttcagttt gtaatagtag
     1681 tattcgtcca tatcatcaac cctcgatatt ttcacaaagc catatttcag taatgtcatc
     1741 agcagaaaca ccacatcgtc ttttttcatt tgcgtggcag tgcacagttc agcgatgctt
     1801 ttttcgccct ctttcaactg gtcgagaatc tgctttttgt cgcgggtaaa ctgtttaaga
     1861 ttgtctttta ccttgtcgga aaccttgtgt gtttcgcgga gtattttata ggtctgtttg
     1921 ttttcggtaa tcatagctgt ttcggtttat tgtgctaata catcaatcat gctttccatt
     1981 tctttgttgg taaacgatat caactcgatg gagtcggacg ggcaaacggg caggcacatt
     2041 ccacaacctt tgcagttgct tttgcttatt tcagccactt ttttgccgtt gaaatcaatc
     2101 gtgcttatcg cactgaacgg acaagcgttt gtgcaggcat tgcaccagtt gcaggtttct
     2161 ttatccacat aggccactat gggttccagt tgtaattcgc ctttggcaat aatggagtgc
     2221 gctttggtgg cggctgcaat ggccgagttc accgattcgg caacattttt aggtccctgg
     2281 caagctccgg caatggtaac accgtcgatt acggtttcaa ccggacgcag tttcatgtgt
     2341 atttcgttga agaaatggtc gcggcctttg ggtaatttaa aaatattgcc gatagtttcg
     2401 tccttatcgg gaaccatacc ggtaaccagc accacgaggt caacttcggc ttcgtgttca
     2461 cgtttttcga gcaataaatc aaccgctttc acgctgagtt tatcgccgga agcttccacc
     2521 acaggcggtt tcttatcgag aaactgcacg tagatatcgc ctttatcgtt ggttgctttg
     2581 taaataagct cttgttttcc gtaactgcga acaccccgtg tgaagtgata gcattgcagg
     2641 ttgctgaatt tggctgttgc atcgagcgag gtttgcatgg cagcagtgca gcaaaaacgc
     2701 gaacaatgct tgttgtcgcc tttcacctgt cgactgccca cgcaatagat aaatgctacc
     2761 gaattaattt ctttatcgtt taccacgagt tgttttccac tactgttttg tagtattgct
     2821 ttaaactcag gtaaggtaat tacgttggaa tgactgtgat agccaaattc tccttcttcg
     2881 ggggtgtagg tgtcgaaacc ggtggccagc aaaacagaac ctaccgtaac ttcgcgattt
     2941 ccggccggcg agttgagttt cagtgtgaaa ttaccaatac tgccactgct ttcggcaagc
     3001 atggtttcgg tgaatagttc aatgcggctg ttgttggcta tttcagcttc gagttgtttc
     3061 agtaagtcgc tgcccgattg tttttcgggg ctgataacgt ccattgtttt cagtttgccg
     3121 ccaatttctt tcgatttttc gatgagatac actttgtcgt tcatgccggc aaggcttagt
     3181 gcagctttca tcccggcaac accggcaccc actacggcca ccgagcgttc cgattttatt
     3241 gtaatggggt tgagggcttc agccagacgt gctttggcaa tggcggcctt tactatctga
     3301 atggcttttt cggtagcccc tgttttgtcg tcgctgtgtg cccacgaagc ttgttccctg
     3361 atgttggcat gcacgtaggt gtaggggttt agtccggcgc gttcgcttac gttacgaaat
     3421 gtagtaagat gcagtttggg cgaacacgaa gccaccacca caccgctcag gtcgttggcc
     3481 ttaatgtcgg catccatgtc tttttggttg gtatcgccac aggcaaacat ggtggttttg
     3541 gccagaatta ccgcatcttc gtctttaatg gcttctttaa ccttctcgat atcaacatag
     3601 tcggaaatat ttccgccaca tgcacatata tatataccta gtttcttctt cattgtttcg
     3661 tgttgcatat tactggttta aatagcttat ggcttcgccg gcggcagcac cggccgacag
     3721 aatggaatcg ggaatgtctt tgggaccggt ggctgtgccg gcaacaaaca cgccttcgat
     3781 atttgttttc gatggactaa gcaatgggct gggttgtgcc acatagttga gcacgtcgag
     3841 ttcaagcttt ttaccttcga aaagattacc ggcttcagca ttggcgatta cacctaccga
     3901 aagcactacc atatcgtgtt cggcttcctt gagttttccg gttgctatat cttcgtagcg
     3961 caacgaaaga tttccgttgg gtttttcggt gattttgcca atttttcctt ttacaaaccg
     4021 aacacccatg ctaactgcct gttcgtaaaa ttcatcgtaa cccttgccaa atgcacgaat
     4081 atcgatgtaa taaatcgtaa tatcggccat gggcagtgca cccatcagca actgagcctg
     4141 cttgatgctg tacatgcagc atatttgcga gcaaatgggg ttgcccaccg aatcgtcgcg
     4201 cgaaccggca caaagaacat aggccagttt gtcgggcact tttccgtcac cgggacgcaa
     4261 tatggtgtga aacggacgcg tgggagccag ttgtcgttcc atttgcatcg aggtaatcac
     4321 attttttgca cttccatacg aataacgcga aattttcagc ggatcaaaca gtttgaaacc
     4381 ggttgcaaca atggttgttt ttgctttcag cacatactcg cggggtttct gcgtgaagtc
     4441 gatacaaccc ggaggacata ctttttcgca ggctccgcaa agcgtacaat tatcaatgtc
     4501 gatggcagca accttggggc tgcaaatgct gaacgggata aaagcggctt tacgaccgac
     4561 cagattgtac tgatattcgt cgcgcaccac tacagggcat gcttcttcgc acaactggca
     4621 agctgtgcaa tcggcctcga ctacgtatcg cggacgacgg gttacccgtg cttcgaaatt
     4681 cttttcaccc agcttttcga tggagagcac atcggtttcg gtaaaaatac tgatgttggg
     4741 gtgcctagat atttccgaca cctttggggt agtgatacag gctgcgcagt cgagggtggg
     4801 aaacaccttg ctcagcaata tcattttgcc accaatcgat agttcttttt ccacgagggc
     4861 caccttgtag ccgttgttgg ccagattcaa tgcggtttcg ccgccggcaa taccgccgcc
     4921 aaccaccaat gcatcgaaat tcaggatttc aggctctttt tgcataaatt attcctgcga
     4981 ttttaataaa tactcgttaa aacttttcac ctgtttcacg aacgattcgc tgcacaccga
     5041 gcaaatggct gccatgcgca ggcgtgcagg gtcgatgttt tgttctttca tcaggttgtg
     5101 ggtttgctct accagatgtc cggttttttc ggtgcaactt tcgccatagg cacagtcgct
     5161 gccatcggcg gcaataaaca caccgtcgaa acctttgtcg aaagcccgca ttatccactt
     5221 tggcttaatg ctgctcgagc aacgcattgc tatggtatag acctgcggtg gataatgcag
     5281 tttaagcaga cccgccatgt cgatggcagg gtctgatatt ttttcggtag agaaaaccag
     5341 gattttcgcg ctttttttag ccatgttccg tggtgttaga tttcgttttt acgcatgtaa
     5401 attttgtagt aaccgttttc ttccaatgta cccagataat cgtgtccctg tttgttgcac
     5461 catttgggaa tgtcgacacg ggttccttcg tcggcagata gtatttccat gatttcgcct
     5521 tcttcaatgg ttccaagggc tttttttgct tcgagtagtg gtccggggca ggcagttccg
     5581 cgtgcgtcaa ccgtttttgc taccactaag tttttcagtt cttcgtttgt cattgtttta
     5641 gagttttatg gttaaaaatt ttgtgtttta atatttcaat tattgaaacc ggtcgccgag
     5701 cggagtcgag gtgccggtgt tataatttct acacttccac cttgccgcca cctcggctcc
     5761 gctcggtggc cggtttggtg ttcggcaatt aaataaacaa ctgcgctgcg ctttcgccgg
     5821 catctgtaac aaatgtagcg gctccagcat aggtcagatg cggatagtcg ataaattctt
     5881 ctttcttgaa ccccatcagg cccatcgaca tttcgcaaac gatgatttca atgccgagtt
     5941 caccggcttg tttgaacatc tcgtcgagcg acagtacgtt gtgttttttc atcagatatt
     6001 taatcatcat agggcccata ccggccatgt tcatgttcga cagtttcagc ttgttcttgc
     6061 cttttggcag cataatgcca aacattttag agatgaaatc tttacccttt gctgatttat
     6121 tcttgtcgcg caaagctgat aatgcccaaa acgagaagaa aattttcact ttggtgtcca
     6181 tggctgcagc ggcgagcgaa ataatcattc cggccataat tttatcgagg tctcccgaaa
     6241 caatggcaat cgaaagctgg tctttgcggt tgttttcgag ttttgcaagt ttcttgccca
     6301 ttttgtcgag ctgagcctgc atctggctga gttgttgctg aagttcttcg ttcatattct
     6361 taattgttaa tgtgttacaa tttaattaag aacaaagtaa gaattttaac tgatataaaa
     6421 tccctttttc ttattgattt gtgttttaat taagtgtgat atatatcaca cttgagtaat
     6481 gatattttta tcaagaattc ttaaagactt ggatgaggaa aattagtgac aacaaatgaa
     6541 atttcaaaat tgaaattttc ccaactctta atttctaatt tagtccttaa tacaacgaac
     6601 actgaggaaa tcgtttttac tggtgaaata acgtgtggca gagccatgca ttatgtaaaa
     6661 gtaccatgat ttttcttcat tgctctcaga agataaccac caatagccac agtactcaat
     6721 atggttgtac tcataaagct ctgtacaata accgcccaaa agaccatcaa aaccacttct
     6781 acccccaact ttaagcgcct tatagccttt agcaccataa ttgccaacat ggcttatcaa
     6841 agtatcaaat tcgttttttg ttggcaaatg ccatccattc gggcaagcat tttttgcagt
     6901 gctccaattg tacaatcttc cgtatttgtc acaatttgga aagctatcat cgtagcaaaa
     6961 cgcaacagct tcagtaatgt aattcaggtt ttctgccatc caggtttgtg accctatggt
     7021 aatggtttta taggttttac catcgcgaga atcggtgaat gtacccgaag cttgcgattg
     7081 tgctttaatt gtaattgcaa ctaaggcaat cgtgattaat aatagtttac ttttcatagt
     7141 tgtcaacttt taagtactgt aacatcaggt tttttgaacg gttaatttgg attctaacat
     7201 attctagtta ttgcacgtcg ctgaaagcat taatattggc agtagtaatt tgccatttcc
     7261 cttccgatgt cttaacccag tttatcgtaa atactccatg cggatgtccg attaccgagt
     7321 cgttcacgac aatatcgaga ccgtaattac ctgtgtaacc ggctctgtcg atactcgacc
     7381 attcctgtag cttttcaact ttcatgtttt ttacgtatcg gtgaaacgga ccgatccagt
     7441 tttttgacag gtctgatgta ttcagcacca ccagttcatc atcaatgagt accacctcgt
     7501 cggcgaacag gtttctgatg gctgccgaat cgttgttgtt ccacgcattt tgccagtttt
     7561 cgataagcga gtcaatgggt gctttttgaa tcgaagatgt ttcgcttgga ttttggttgc
     7621 aggaaatcaa tattccagct acggcaagta aaagaatcat ttttttcatt gttttgtttt
     7681 ttaagttaat aattatggat tatttgttgg tttaataaaa ttgctttttt cattctcatt
     7741 taatctttta cacaacgtat gtattcacca tttacatagg cagaatttgt tgaatacaat
     7801 gaaaccacct cattgaatat tgcaaaactg tagcaatcgg atgttctcca ccacgtcatc
     7861 attcccattc catcaaacac cccaccaagg tcagcattgc gcataccccc ggggtaggca
     7921 gtaaaaccgc ttttgtttct gaatgaactg taatcggtgt taccgggtgt gccgggtacg
     7981 gttgaattat cccacaatgt ggttgatgcc atggcttttg cgattttatt ctcgaaattt
     8041 gtattgtcgt aattgtaata gtggctaatc agatagttct gcatggtagt ccattcatcg
     8101 tagcttgcaa catgccagcc ttcgggtgct atgccccttg ggtcgctaac tgcgtaccag
     8161 ttatacatta ccccgtaagt atctgcgtta gcagcatagt tgttgtaata gcaaaatgca
     8221 cctgtgttta gactgtacca ggttgaatta tcaataacat tgggaattgg gtcgccattg
     8281 ttatatttgg ttgtcctgag gttttcaacc agccagtatt gatcgccgat ttttacactg
     8341 tggtatatat ttccatcagc atccattgcc cattcggggt tgaggcttac gtgtatatag
     8401 tcggtttttg tgacggtgga gtctccgtaa ttatttgttg ctgttagcgt tactgtgtag
     8461 ttgccgggag tttcgtacac atgataagga ttctttgcgg ttgacgtggt gttgtcgcca
     8521 aaatcccaaa aatacgatgc gccattgatt gtggtatttg caaaatagac ttcttcgccg
     8581 gtataaactt cggtgtcata tacaatgaaa ttggcgatag gttttccggg gcggaatcct
     8641 gatacggagt agaagacact tacgcgcttt gaagttgtca gccctgcaag atcaattacc
     8701 atgatatcaa ctattgccga atcgagatcg gtttcgaaat gcaggcttat aatttggccg
     8761 ctcgagtaat tcaccgtatt cgggtttaaa gcgatgatgg gcaattcgac accatttttt
     8821 ttaatagaaa tttctttaat gccgttgggg gcagctacga gaaacataat ttcgccattc
     8881 caatattgat cagtgttgct atagggttta accacagtgt cttcagaaac tatgtactta
     8941 tgttgactgt agctccaatt ctcaagcaga atccatggcg caggcttatc acctatgtca
     9001 actttctcac atgaatagaa cattgcaaac gctattatgg aggcaacaat aattatgtgg
     9061 tttctaatgg ttttcatgat gattatgttt ttaaattatt cataattacg gttaaacaat
     9121 ggccatccgg tttcgagtcc ggctttcaaa gagaaataca gtagaaatga acttcccatg
     9181 ctttcatgat aggggcgtat tcccggaagc atcccaatct cgaacatcca ataccccgaa
     9241 aacgcattca gacggttcat ttcgatccct atgctgaaac cgggcttctg aatatattgt
     9301 gttggaatgt aacctggaaa aacctcattt cctttttcgg tgagaaaacg gtagttaatg
     9361 aaaggtgcat acactatatc aatgtacatt ttacgcattc cgatccggtc ggttccttca
     9421 cgggtgccgg gcgatcctac attaaatact tttgtggtac caatgccaag atgaaacatg
     9481 gtgtaccggc ttgaactgag gatattttgc gtactatagc tgtaataata tgattgcaat
     9541 tgtgttgggt atgggtaatg gcatacgggc acagtgtcga gaccgtcagt cgggaatgcc
     9601 agttgcatat tggttactcc gaaacggatc tggttataag tagccgcgtt tccttcgcca
     9661 taagagtaca catcgtacgc atttcctcct tcgtaatctc tgcccctgta aaagaaagag
     9721 ccttttctgc ttactttcga cgagaatgtc aaagctccgt caaattccaa gcgactgaaa
     9781 aagttgtcga gcagctggta ttcgttttca tcgctgaggg caccacggaa ccaatcctgt
     9841 atggcgcgct cgtaatagac atccaaagca atgcggggtt tgaaatagtg cagcgaaaac
     9901 tccagcgttt ctccataact gcctgccatc gtaaaccacg aggctgccac cacaaacgag
     9961 aagtttctgt tggtgggagg aggaaccagc ccgaaatgtt gccgttggtc ctgtgcattc
    10021 agagaaatcg cccaagcaat aactgaaatt atgacgaaaa tgatcttttt catgattcac
    10081 atttttattg tttcagaatt agtaattttt aatgcagcga acactataac catttgtttt
    10141 ccatgaagtg tattgttcaa ccaaatttgt tgagaatgta aaacaataag aatatgccat
    10201 ttcattgtct tcaaatacgg tggttgatga ccatagaatt gacgtccact tgagattgcc
    10261 aaactcatct ttttcgctat tataaaaacc tgcaggtaat attcccaagc cagattcatt
    10321 ataggagtat tcaatatttt catcataata aaatgctcct gaggctttca gttttgcacc
    10381 ggcattagcg cctccattat tcataataag taagttccat tcgatagcat ctggcaggtg
    10441 ccagccttca gggcaagacg tcattgcagt ttcccaatcg tataagtacc caaacttttt
    10501 cacgtgtttg tccttgtatt cataagcaaa ataatttccg ctgtctggag caaaagccag
    10561 gttttcggca agccaggtaa catgattaat gataacggtt ttgtacactt tgccgtcgcg
    10621 ggtgtcggtg aagcttccaa actcctggca gaacccgaac gatacagaca tagcaagggc
    10681 taacaaaaga attgcttttt tcattttttt ctggtgttat tgcttacttt gttaaggcgt
    10741 cagcactcct ttaattacaa tcaaatggtt ttctgctttg cagtggaaag aatattttat
    10801 ctcacttttt agtgaactga tcgagcattt cgggtaaaca agtgctaaaa acaatcccgt
    10861 cgaaatggcc gccttcaatt tcgatgtagt gatatttgat ttttttcttg tcgagctctt
    10921 tctttagctt ttggtgctgc ggtacaatga agtcgaattc gtcttttgtt gccattgcca
    10981 tatatacaaa ctgattgctc ccttttttca tgttgctgac aatttcgaaa acatcggttc
    11041 cccgcgattg gtaactgttt tcatcgccaa aagcttcgcg cacactgctc atgataaaat
    11101 tcaggggcga ttcaggtgta agcggaatat cgtagaatgg tgcattgatg gctccacaca
    11161 tgtctgcaat aaaagtaaac cgtcccgggt gcttcaaccc gacaagaaga gctccgtagc
    11221 cacccatcga aaagccacct atgccttgcc acgacgggtc ggttttgtat ttcttgtcga
    11281 tgtacgtaaa caattcttcg gtcatgaaac tttcgtattt agccgtagga ttcgagaaag
    11341 aattaacata ccagtttgta ccggcatctg gagttactac aataatctgc ctgtttttca
    11401 gttcagatgg caaatttgtc tcaagaaaac tatcgttgtt gcctccccac cagtgcaaca
    11461 gatacattac gggataaacc tgcgtagtat cgtaaccagc cggcagaatc actgtggtgg
    11521 ccactttccg gtttaaaatg acagattcaa ttgaatctgt tgtaactgtc tgcgctttag
    11581 taaggctggc agccaaacag ctaatgaata atataaagta aatctttttc atgggcttag
    11641 tgtttataaa ttttatttat ggcttcagtt ctcgactgaa cctggagttt ttcgtaaata
    11701 ttccgaatat gtgtccggac agtttcgatg ctgataaaaa cttttgcagc tatttcctta
    11761 tagcggaacc cctgtgaaag cagatccaga atttcgcgtt cacgttggct taaaacatgt
    11821 atttcatttg aaattctggt tttaagactg aattgttcaa ttaccctacg ggcaatttgc
    11881 ggtgacatgg gtgaacctcc tgaatgtatt tcagcaatag ccgaaattat ttgttcaggc
    11941 cgggagtttt tcagaagata ccctgttgcg cctgaacaca aggattgaaa caccgactca
    12001 tcgtcatcga aaatggtgaa catcacaaat tgtatttcgg gaaattttga ttttgctgcc
    12061 tcaacacatg atattccgtt catgcccgga agttctatat ccattattac cacttctggt
    12121 gtattgactt ccaaatcatg caaaaaagcc tcagcattag catacccttt taccagcaga
    12181 cagtcaattg cttgcccgac aatattttcg accgattttc tcagatcgtg gtcgtcttca
    12241 actatgctaa ctgaaatcat cgcttttttt attacaaatg tacgctcact aaaaactgaa
    12301 tacaatacta ctttagtggt aatttttatt tggtttatag cggaactatt aatgttactg
    12361 ttgtcccttt gttcttggcg gaatcgaatg taatgctacc cccgcaggct gtagcccgct
    12421 tattcatgtt cccgatgccg gtgctgaaaa ctcctgcttg ttctaagtca aacccgatac
    12481 cattatccgt aatttgcaat ctaatttgat tttcagtcag gctgatttca acaaaacacg
    12541 atgttgccga agcgtgcttt acaatattgt gcaatgcttc tttcaggata agaaaaaagt
    12601 tctgccttaa taaaggcgaa atgtttattt cctcatgcac cgaagggaaa tttatatcac
    12661 attctattcc tgaatcttcg aagaaattgt tacagtaatt gcgaatatac gccagcagcg
    12721 agctgatgtt gtcgtatttc ggattagtcg accagatgat ttccctgaaa tcgtcgataa
    12781 cccgccgcga aagcaacgaa atctgagtta gcttattcag agaactctga ctgtctttaa
    12841 tttcgctttt caacgattcg ctgagcaacg atatcttcga cagcgatgct ccggcaccat
    12901 cgtgaatgtc tttcgacaat gctgatcgta ttttctcaat ttcttttagc ttgtttagct
    12961 cttcaatttt ctttcggtat ttcaggtaca gaataaatct gataattaca gccagaattg
    13021 taacgactgc ggcaattgcc agtgcgataa accaccatgt ttgccaaaat ggaggcttaa
    13081 tggtaaaacg ataggcgagg ggttgattat tccaaacacc atcgttgtta catgccttga
    13141 ctaaaaatgt gtagtctccg ggttcaagat ttgcatattc cgccttggtt tgctccgata
    13201 caggcgacca atctttttct aacccttgca gcatccattg atacttcaca gcatctggat
    13261 tagtaaaaca tatagcttca aattcgaaag taacatggtt cttatcgtat ggcaggcaaa
    13321 gatttacagg taaacgaaac caaggactta ctgaatcgca gaatttgcgg aattccgaac
    13381 tttcccagtt tacatcttta taaaagagtt ttatttttcg gatatttact atgggcggtt
    13441 ttgtattggg tgttaactga gatggatcca gcctgatcaa tccctgggtg gtcgcgaaat
    13501 ataaattccc ctttttgtcg gtattaatat tttgtgcacc aagcgatgac cctataaatc
    13561 cgtcaccttt cccgtaatat ttaattgatg aaaccgaacc atcctgtgta aacgaaatca
    13621 aatcaaaacc ctgattgtag ccggcgtaca tatttccctt gttgtcgaag ccaagagaaa
    13681 catagtaacc tgatgaaagt ccgtcttcag tagtcaataa ccgctgtccg tgggtatcga
    13741 caagcagaag accaccgtac aaagcaatcc agacacggtt ccatttgtca acagcgatat
    13801 catcagggct tatactgttc ttgggatagg ttgtatagga gtacattttt ccatcccatt
    13861 taatatatcc ggcatttgca gcaaaccata cattcccgat attgtcgctt gtaatactca
    13921 gtatagtccc tgcttcggca cctatttctt cagcacttac tacagcagag gtttttccat
    13981 cgaatcgtat cagggaactg ccattggctg aaaaccaaat gtatttgcca tcattatacg
    14041 cgatatacgg aattacatcg ctttccagat caaacatgtt gttcaattga taaccattac
    14101 tgctgttagc atcaaaaagg ccctgatatg taagcactat tacctgatgc tcgttggttt
    14161 cgaaaaatcc caaagtttca cgattgtcgg gtgcatcaat atatagaatt tcggagccgt
    14221 taaatttgca aacctgatct gttggagtag agaaccagat gttaccctgt gagtctttaa
    14281 aattaaagta aactttcgat gatttcaatc catctttttc gttaaggcat atgaattttt
    14341 cccccataaa ttttattagc ccgagccccc ttgtagccaa ccaaatgtta ttttcgttat
    14401 cctccgtgac ccaaaaaaca agctcgatgg gcaaaccgtt ttgctcatta aaatgcttga
    14461 cctcaccatt gctgcttact cgccataatc caaactctga aggaacccag aggttgtttt
    14521 catgatcctc acaaaccccc gataaaaacc aatacccaac ttcagcaggc atatcgagat
    14581 aggtgattac tccattttga agggtgaaaa tagaaagctt ctggatttta gcgtcaaacg
    14641 acagaaaaaa ctgggtgtga tcggcagttt cataaaacag atttaaccaa ttctccgata
    14701 cttcagccgg gaccccggga ttgtattcat tcagcacggt gtcgtttact atgtacatgt
    14761 tccccaaggt atttactatg atatccccgt ctccattttt tacgaccgaa agcaataacg
    14821 attcaggtgc atttagaaaa gggttcacgg gagtgatatc atgaaaccgg ttgttttcga
    14881 aatacaataa ctgtcgggtt tcatcagcat tccataaagc aatccagagt cgcccttttt
    14941 tatcgaaggc agtggaagcc cattgcccgg gtaatgcaag accatctttt gatgtgtagt
    15001 tggtaaattt tagtccgtcg taaatactga tgcatttcag gcttagaatc attaacctgc
    15061 cgtcgggcaa ttcgactatg ctacctgtat tgttatcacc aaggccgtct ttttcggtat
    15121 agttgtcaaa tgtattccca ttgaacctgt aaattccatt gcccatagtg gtagcccaaa
    15181 gatatccccg gctgtcctga attatttttg taacatcatt cgacttcagc ccttgatcgg
    15241 cgccaaactc ccaaaactga tattgctggg cgtttaaagt tacagattta attatcagaa
    15301 ttataaataa aacattgaac ttcatgcgat tcatgccctg aaatattatt ttttgtcgaa
    15361 gataatgata ttatttgttg aattctaata tataaaggct aagttgtttt ttaaacttta
    15421 aaatgtgtat gaatatcact gattaatatc attttgcctg ttgattaata ctatagatag
    15481 catattcgtt tgtattgcta attaattttt gtattttgtc tcgataattc aaagagatga
    15541 accataactg cagtttttgc gaacataaat cggctgctgc tcacaaactg aataatgatg
    15601 aactgtctgc tttgtcagcc aactgtctta cggtgaagtt taaaaaagga gacaatatca
    15661 ttcgtcaggg cacatattca accaatgtag cctacctgaa aagcgggctc gtgaaaattc
    15721 atatggaagg accctatcac acgcaggtag tgcgtattgt gaaaccccgc aattatcttg
    15781 gcctgcccac tactttcggc gataaaatca atcagtactc ggttactgcg attgagtttt
    15841 cagaagtgtg ctttatcgat attaccacat tcagggattt actcaacaac agcccggcat
    15901 tctccaacca aatactgata gagctttgca ggggtgaact cgaatcgtac cagaaatgcg
    15961 taaaccgcac tcaaaagcag gttcgcgcta aagttgccga tgtattgcta gatttctgcg
    16021 aaaatattta taaatcaaat tcatttcagt tgaatatcac tcaggaagat attggtaacc
    16081 ttgttgatgc cacaagagaa agtgtatcaa ggatttggaa tgagtttata aaggatgata
    16141 tcatcagggt tgatggaaaa accatagaaa ttgtcaatat tgctaacctg aaaaatatca
    16201 gccgtaaagg ttaaagccca atgacagttc attttacatc aggtatccga agcaaatgga
    16261 tgcagcttac agtgtacgac aaaaaacacc ctgttgttct aatactgctt gttgcaattc
    16321 cagtactgtt gtggtttggt tatctgaata ttatgaactg gaccgatgga agcatttcga
    16381 atggcattcc cggattaaaa ttctggctcg acagtgaacg atatatcgat ggagcgacca
    16441 gtattgcaca tggtatgccg ttgcagggaa gggaaataca gttcacaggt tatatgctcc
    16501 tgattgcatt ggttcaaagc ctgcaccttc ctttatcggt tatggcagta atccagattg
    16561 ctattgcatt gctggcagcg gtggcgctgt acgattcgtg tcggttgctt tcggggtcgc
    16621 gcattgcggg attgtttgct gcagcattgt ttttgtgcaa cccatttata gcgagatggc
    16681 atatttatat aatgaccgaa tcgctttaca catctctgat agtgctttgc ttctggtcgc
    16741 tgttgcggct gcttataagg aaaaaacccg ttgattatat tgtgtcagca atttttctga
    16801 tttctgccat gtttgtgcga ccgaacggct ggatattgtt acctgtgttc ttcattgtgt
    16861 atttatactc tttcaatctc aaaaaatggg tgttcataat atcggttttc gcaacaattg
    16921 tggcatttac gctgacaatg gcgaaagttc cggttttcag aagctctgtt cagattacta
    16981 ctcctgttga aaacctgcaa aaaggtatta ctgtttgggg tcacggtgag ttgagtttaa
    17041 aaatgcctca ggataaggat attgataaaa cccaatggac ttcgggttgg aagtacattg
    17101 ctaggcatcc atgggtgagc ttcaaactgg ctgctgcaag agctggatat tccctaattc
    17161 acatcagacc ttaccacagt actgcttata catggcatgt ggctttgtgg gtatttccgg
    17221 catatttgct tgcactgatc gcgtttattg gctatcgtag aaaaacagct gtttatgttg
    17281 ctttaatgat cattttagga cacttgctgg tagtagcact tagctatgct gagcacgact
    17341 cgaggttcga catttatata ttgccggttt tttatatttt tgcaggactt ggtctattgg
    17401 agctactgaa aaaagtaagt tctttaattt ccccgaaagt cgaccaagct aaattataaa
    17461 ttgctcacgt aagaaggctt cagataaatc gcagtaaata tattgttttt gagtactaaa
    17521 atctactttt gtatttcata gcccttgtta atatgagaaa aatatttgac agattaatag
    17581 tttttgttgg aagccttgaa attaaaaaag atcaattgtc gtttgacttg agcatcctgt
    17641 ttagtatcgt tttttatcaa atccttacaa aagttgcggg aaaaactgta acacagatgt
    17701 taagcccagc agcaaccttg ttcttgtgta tgttttttct actgatttta tcgatgagtt
    17761 gcgggtattt gcacatgctt tatgcccgac aaaggcggat atggggttat ttaactatac
    17821 tcgttacggt attcctcatg atgttcccac tgacggttat gagtagaatt gctgatgccg
    17881 gcgatttggg gatgatagca acattcttga cgggaactgg ttttgtcatg gggcgcatca
    17941 tagcccccga taaaatggca ttaaccatta agcgatatgt tttgttgctg ctgcttgttc
    18001 ctgcttcggt cggtttgatt ttctggatga taagtttcac aggagacgag ggttataaag
    18061 gagtactgat atgtgttttg ggctttgccg cagtggctgg cttgggattt attatccaca
    18121 aattattctc ccgaagcgag acagctttaa acaatttctt tttatcgccc aatgcgaaag
    18181 gtattgcaaa gttcctgatt tattttttct ggtgcgtggc agcctttgct atatgcactt
    18241 ggcaggaagt acttactgat gcaccacagg attccggatt ctaccggata tttatcctaa
    18301 cactttcagg tattattccc taccgcattc tttgggcttt tatcccaccc cgaaagctgg
    18361 cgacaaccat tattgcaaca gttgtgctgg ttattaacat gctttttgtt tacatggggt
    18421 gacataaatt aaataaagac ttaaatacta cttgcggttt tacagtttaa tatcgggcag
    18481 cgacgtaaac agttgcatcc ctatagttgc attttcttta atcactttaa aacggtaaaa
    18541 cgaaggaata aatacattat ttggatcgat aagaaatcgc aggcaatgct ctgggatgta
    18601 ttcaatcaat gaggctgccg gatacacctg cagaccggtt ccaaccacca gaaatatatc
    18661 ggcagttctc acgatctcaa cggcatcttc tatcaatgga acagcttctc caaaccatac
    18721 aatgtgtggt cgcagctgag agcccagcgg acagaggtca cctacattca attcagtccc
    18781 attgatatcg accacaattt cgggatttgc cgtgctgcgc gcttttttta tttcaccgtg
    18841 gagatgtaaa acccatgtac tgcctgctct ttcgtgcaaa tcatcgatgt tttgagttat
    18901 tatttgaaca ttataaattt tttcgagctg taccagaatt ttatgcgctt cgttgggctg
    18961 cgcccgagct acattcttac gccgctgatt ataaaaatcc agcacaaggg ctttgttttt
    19021 tttccaccct tcaggtgatg ctacttcttc gattttatgg ttttcccaaa ggcctcccga
    19081 atctctgaaa gtactgattc cgctctctgc gcttatgcct gcacctgtta gtacaaccag
    19141 ttttttcttt ttcatctgat atgaatttaa tagtgtaaaa atagcatgaa acccgattat
    19201 ataagaaaaa aatgactcaa atatgctcat gcaaatccgt atttagcgta ataatcgaat
    19261 ttgcggcatt aatcttgcag atgggttgta attttaaaag caaatagtat ttttgtcgaa
    19321 ctaattattt aatgaatatg tttctagaag gaattcttgc catatcggga caaccgggtt
    19381 tgtacaaaca ggtatctcaa tcgaaaaaca gcattatcgt ggagtcgatt gaaacaggaa
    19441 aacgctttcc gacattttct gcagcaaaaa tcagtgcttt gcaggatgtt gccatctaca
    19501 cttccgacaa agaagttcat ttggcagagg ttttttccaa cgcttataat gccatggagc
    19561 aatttcccaa tatcagtccg aaatcggccg ataaggaatt atatgcttat tttgaagtga
    19621 ttttaccgga ttacgaccgt gaaaaagtat atctttcaga tatccgcaaa gttgtaagct
    19681 ggtttacatt attgcataaa gccaacataa ttacccgtga aaaccttgat gcatattttg
    19741 aggaagttaa aaatgccgaa gctgctgaaa atgaagcaga accagccgag gagtaattct
    19801 ttgttttatc gattagtatt tgaatgcgat aaaaagtttt tcatcattat cgaggcgata
    19861 taaacccatt acatcttgcc ctaatgcggt tacggggtca aaaatctcaa ctctgaatcc
    19921 tgccgatttt agtctgtcgg gataatcttt accatgccat cttacgtggt cgtactgacc
    19981 gaatactttt tccctttctg ccggtgtggt tatattttta tcttcgaaag ttgtttcaag
    20041 cgattgattt ataggtacct gcataattgc ccatcccttg ggttttagaa tgcggtaaat
    20101 ttctttcatg gcatcaatat cgttttcaac atgttcaagc acatgattgc aaaaaaccac
    20161 atcgtatgaa ttgtcgggca aaggaatatg cattacatcg aaatgaagct ctgcaatagg
    20221 cgaaaccata tcagcagtag tgtagctgag tttttccatt tttttaaacc tcctccttag
    20281 cgattgctcc ggtgcaatgt gcagtaccgt atatgtttta ttgaacatgt cagtatagtc
    20341 tcgaagatat atccataata agcggtgtcg ctccagcgaa aggcaacggg ggcacaaacg
    20401 attgtcggca cctttattac cgtagggtag catcttgcgg aaacgattcc cacaaatcgg
    20461 gcattcgtaa tttttccctt tgtaaaatgg cgctaccatt gccgcaaaaa gataggagaa
    20521 ctgtatcaac acaggccggg gaacattcct aactaaaaag cgcatcagct tcttcatgca
    20581 atcagttctg ttaatgtaat ttcttttttc atattgattt ctatcagttg cctgccgaaa
    20641 acataccata cataaccctt gacacaggtt ttccccataa ggcttataag ttgcatataa
    20701 tcgaacacct gattaatatg ttccgcacta cgtttattac caaatttata gtcaaccaca
    20761 gcaatatggt cgtcgaaact cattaccctg tcaggtttat gaatacttcc atccggagca
    20821 agaatccagt tttcgcggtg caaatcagtg tacccagtga accagtcaga aattttaggc
    20881 tgggaaataa tttcggcaat tttttttgta agtgctggct tttctgatgc cggaagtaaa
    20941 ttctttgcat gtacctggtt tattgctttt tcaatatcac cggtattgcg gatgttttcg
    21001 aaaattttgt gataaagaat gccctgttct accggcaggt tttcttcagc ttcaagcaga
    21061 cgggtgtaat cgccatgggt tacggcctgc tttttatttt taactaccgg atgaattgct
    21121 tcaatgctgc agcgtttcga tgattcttca atggcctggg tatgtccaac ttcgaaacaa
    21181 ttggtagaaa catcgtaatg ttttcgcaaa tcgttggaaa acccatcgtc tgaggaaaac
    21241 tttccctgaa gcaatgtttg taaaaatccg gaaacactgg actcaatatt atcgtttctc
    21301 cgagaagggc aatgtatata cattgccttt ttggcacggg taaaagctac gtaagccagg
    21361 tttaatttgt cgagataaca attaagcttt tcttcaaagt attcattgca gaagtcggta
    21421 ttacctaact ctgcgttgta tttcaggggg atgaacggaa cagcatcgta catgtgaccg
    21481 gaggtgtctg cccaaattat tggataatgc tccttgggct ccagataaaa ctcgcaaaaa
    21541 ggtaaaaaca ctacatcaaa tccaagtcct ttcgattggt gtatggttaa tatacgcagt
    21601 gcattgtccg aatctggcat ctgaagcgat tttcttgagc catctgattc ccaccattcc
    21661 aaaaaaccgg ataaacccgt tccttttgat cgcgaaaagt ccgctgcaca atcaacaaaa
    21721 ctatcgataa aaggaataca atcagaaaac tcgtttaatt tgcacatggc gataatgctt
    21781 tctgccagtg caattaaatc ggttccggca gaatggtcga tcagcgttcc aaaatcgtcg
    21841 ggaaaaacat ctgtaagggt ctgaatgcca gaatgtgcgc tgatgttcgt aatcttgtct
    21901 tcctcgcctg taaagtgaat caggtaattc aaacaagctg tgtacaatgc tatcgtattc
    21961 ccgggttcga gaatataacg cattattcct gttattacaa ttatggcagg gttagaagaa
    22021 agaaccagcg attcactcga taccacagga tatcttaggc gttcatctgg tttttccttc
    22081 gactgatgtt caagaataag agcagcagca cgtttagctt cggtatgagt gcgaacaagt
    22141 atggcaatat tcccggggtt gaagttttgc agcagaaggc tgtcgattct ttcaatggtt
    22201 tcctgcatcg acgaaagctc ccaatcggtc ccttgtttag gttcatcagt gaagaattta
    22261 acacgtacat gtccgccagg cccggctttc acattttggg aaacttctgc ataattgctg
    22321 gtaattgatg taagtagctt gtggttactg catttctcat cagtgttaat ctgactgttg
    22381 aaatggtttt gtatcatctc aggtactttg ccgaaaatag tattgttgaa agtgacaata
    22441 ttttcaaggc ttcgccagtt ggcggataga ttctcccttt tcacgctgaa ctgtgtgttg
    22501 atgtcctctg caagaatttt ccagctgcca ttgcgccagc ggaatatcga ttgcttaaca
    22561 tcacctacca ccaacccata gttgttgcca tccgaaatac attctttaag gagcggaaga
    22621 aaagcctccc actgtttaat acttgtgtcc tgaaactcat caatcagata gtgtttcagc
    22681 attatacccg ctttttcgaa aataaaagga gcatcgttgt tttccgacat ctccgctaaa
    22741 aacaaagggg tatcgccgat aaaaaaacga tcgttctcat tcaggtagtc tgcaaggcgt
    22801 cgtttgccgt caacgataag ccccagcagg taaatgttgt ttcttatttg tttggctgta
    22861 aaaaactcct ttcgtgaagt attgcaccaa tccaggtatt ttttactcaa tgcgttaagc
    22921 aatggataaa cttttcgcaa taccgcttca ggattaggag cttttccgat aaatttatct
    22981 tcattatcga ttccatttaa aaaatataca ccgggttctt tgaaaccatt tcgtagttta
    23041 agcagaaaac ctgccggccc attgtttttt tgatgaaaat tctctggtgt aagattgtgc
    23101 ttaatcagaa tttcttcagc ctctgaccct atttttttaa taaagttttc gtgtttgttt
    23161 tcaatagaag ttaccagaga taccagcttt tttacatcat tgcgacagag tttttttaat
    23221 cggtaaattt tttcgatata tgcaaatttc tctttaaaca actcattgaa taactcaact
    23281 agctccgacc ggaagtccca gctacccgaa acgtccattt ttcgggttac cacttcgagt
    23341 acccactcga gcaaatcttc atcgtttgct gtatcatcaa gcatttggtc aacaacctga
    23401 tttacaattg acccggaatc gagctccagt tcaaaatttg aattcaggtt catttctctc
    23461 atgaaatttc ttagaatacg ctggaaaaaa ctgtcgattg tcatgtaaag aaatctccca
    23521 taatcgtgca gtatcatatt cagcacagct tcagcctttt tccttacaat tttctcctgt
    23581 gcaggagggt tcccatgaca aagctcagca aggaaatcgg aattatttcc cgatgaaaga
    23641 ttgtacagct cattcaggat tctttccttc atttcttcgc ttgccttatt ggtaaaagtt
    23701 actgcaagta tatgcctgta tgcatcgggc tgatttacca gtaatcgaag gcaatcgcct
    23761 gttaggcggt gggtcttgcc agcaccggct gatgctgagt atacatgcag cagcgacatc
    23821 agaaactcac tgttaaattg ttggtttcac attcggtaac agtgacagga ataatccttt
    23881 catcagctag acttataaaa cgcacactgt attctcctac gggtttggcg acacaattgg
    23941 atgcatcgtt ggcgagggta aaagcaaaaa catcatttac atagacttct gtaattcttc
    24001 cactgtagtt acttacgcaa atttcacctg tgtggttaac ttcgcagctt tcttttacac
    24061 cgcatgaggt aagcaataca atggctacaa aaagaggtat aagaataaac ttcatttctt
    24121 tagtttttcc aaatatacaa actttagcct atctttgaac aaaatgttct aagctttgtt
    24181 ctgaacaaaa ctatttttct tatgaagtat ctgttattat caatactact tttacctgta
    24241 tttctgctga attcatgtga cgttgatgac ccacctgatg atccggacga taacgacaca
    24301 ataaacacta cagctatagt gaacggaagt tttgaacttt ggactatgga atcgcagaat
    24361 aatgttgaat tcgaaacacc cggtggcggt tactgggcaa gtttgaataa ccttgttttc
    24421 ttaggaggtc ctttggttgc taccaaaaca acaaactccc ataccggaag ttatgcattg
    24481 catctcgaag caaaagcctg gggcgacgac tgggtgattc ccggtataat tgctgccgga
    24541 atattcgaca gtaatcagac tattggtgaa aatcttgtta tgggaagagc cataactgtt
    24601 gaaccgactt caataagtat gtattaccga tatcttcctg ccgacaacga tacagcggtt
    24661 atttattcca gccttacaaa atacaaccag aaccttcagc gtcgcgacac cattgctgag
    24721 gcttatctga caattgtgga tgtggcaaac gaatatacac atttagtatt gccatttgaa
    24781 taccgcagcg cattgcacga tcacgataca ataaatatta ttttccttac cagtatctcg
    24841 ggcgataaaa tgaaggctca cgaagggagc atactcgaag tggatgatat tgaacttata
    24901 gttactgaat aatgaaatat attcttactt accttatttt tttcattatt tctgtttcag
    24961 ttttttcaca gccagttgtg atacttgccg gtgggaattt cggaggtgct attcctgaca
    25021 ctaaggtagg caattcaacg ggatccggtg gcatcggttt tatagtgggt gccggaattg
    25081 actttaaggc aggcgatagg ttttatatca gtccatcgtt gtcgtatgtt gcacggaggt
    25141 tttcttacga gtctcatcag accaaagaca ctgttgtaga aattgaattt cagggttcca
    25201 cagcgcatat accaacctat tacacagcga atgtttttgg taacgtcttt actcataaca
    25261 ttgatctttc ggtaaatgtg aactacctga tacgcgagag atttctgcta aatgccggtg
    25321 catcgggaaa atatgctttt gccggaaacg acaataccca tattgttgtt acaattggcg
    25381 aaggtggtat tattgatgat atcattaccg accagaataa ctggaataaa attaatcggt
    25441 ttgatgttgg ttttgacctt ggtgccagct ttttactttc cgatgatttt actttattgg
    25501 tttcgggtga aaggtcgctg acacggtttt ataatgcggg ttatatgaaa acggaatcag
    25561 gaggccctgt atcattttat tacacagcca tgataatcaa acttatttat tcggtaaact
    25621 tacataaccc gaaatttcca gcaagcagta tttgagtctt gagccgtgtt tactgcatca
    25681 tttcgatgct gtttacaatt cgcagatgaa cttcatccac agtgcccata ccatctaccg
    25741 ggtagtacaa atgttttatc ttataaaaat caattacagg aaatgttttg ttgtgatatt
    25801 cctgcaagcg tttttcaata gcttcgggcg tgtcgtcgct tcttttctcg atttcagcac
    25861 gcttgagcat tctttcaata agtatttgtt gctcaacttc gaggccaata atccctgcga
    25921 gtttcatttt gttcttctgt aacaggttct ccagaattgt tgcctgtgct gatgtccgtg
    25981 ggtaaccatc gaataacatc ccttcgcaat gaatattttt tgcaataaaa gcttcaatta
    26041 tacgcgccat aacatcatcg cttaccaaca atcctttttc aatgatttgc tgcgcttcga
    26101 gccccaatgg tgttttattg gcaatttctt tgcgaagaat atctccggtt gatatatatt
    26161 ccagatgata cttttttaca agcatttgag cctgagttcc tttccctgct ccaggagggc
    26221 caaaaatggc aatgtttaac atgttaatta ttttaaggtt caataatttg atacaagtgt
    26281 ttcagatttc ttccatatcc gttataatcg agtccgaaac caaccacgaa tttattcgga
    26341 atattaatcg caatgtaatc gatagggata tccttgcggt atgcatccgg tttaaacagc
    26401 atggtagcaa ttttaacttg tttcggctgt tttttcatca gtgtatggta tagtttttcg
    26461 atggtttctc cggtatctac aatatcttca accacaacta ctgttttccc ttcaatcgat
    26521 tcgttcagtc caattatttc ttttacatcc cctgttgtgg cagtacctga atacgatgaa
    26581 agctttacaa acgaaatgtg gcaaacattt tcgaagtgct tgaacagctc tccggcaaac
    26641 ataaacgaac catttaatat gcataaaaac acaatctgtt cttcggaaag ttcagtgctc
    26701 atacggtcag ccagttcttt tattgctttt tccatcttta ggttgtcgat gaaagcaacg
    26761 aattctttat cgtgtagttt tatagatttt tgatttttat atgtcatatt atattatttt
    26821 taaccttaca aatatagtga ttacatttaa aatttaatat gaaaccaata gtaagtatta
    26881 tcatgggaag tacctccgac tggggggtaa tgcagaaagc tgcttccgct ctcaacgatt
    26941 ttaaaattcc cttcgaaata aatgctttat cggcacatcg cacaccattt gaggtggaaa
    27001 aatttgccac cacagccaaa gaaagaggag taaaggttat cattgccggt gccggtatgg
    27061 ctgctcattt gcccggtgtt gtggctgcct ataccacagt gccggtaatc ggtgtcccca
    27121 ttaaaagctc atttgatggg ctggatgcgt tgctggcaat agttcagatg cctccgggaa
    27181 ttccggttgc cacggttgct gttgacgggg cgtataatgc tgctgtgttg gcattacaaa
    27241 tgttggcaat taccgaagaa aactttgcaa aaacacttgc tgaatttaaa aataacctga
    27301 aggacaaaat tgttgctgcc aataatgatt tggcaaagtt cacatttgat tacagggtaa
    27361 ctgaatgaaa aaaattattt cactgatctc gttttttatt ttggcgaccg gtgcttatgc
    27421 cgatggcgac ttgctcactg ccggtgcccg ctatgcagga atggctggtt catctgttgt
    27481 attacccgat acatggtcgg tttttggaaa tcaggcaggc ttggcctacc ttcagaatcc
    27541 atctgtgggc ttatcttacg aaaacaggtt cctactacct gaaacaagtt atggtgcttt
    27601 ggcatttcag atgccgcttc tagctggaaa catcggcttg ggcattaatc atttcggact
    27661 tacggaatat cacgaaaaca aatttgcact ttcgtatgcg cagcaattgt tccgccgaat
    27721 ttcgatgggt gtgcagttcg attatatttt tctgcatcaa cccgaagact atgggaacct
    27781 gcatgctttt tcttttgaac tgggtttaat agccaaacct aatgataaaa ttgttattgg
    27841 gattcatgct gctaatccga tgccggtaaa aattttaaac accgagaatc aatatttgcc
    27901 aacagtatta aaagttggtg taggttatgt gttttctaag agcgtaatta ctactttcga
    27961 ggcagagcag aacctgcaac tcgaaaagcc agtattcaga ctgggttgcg aatacaattc
    28021 ggagcgtcat tattccattc gtgcaggtat tgctacaaac ccggtgcttt tttcactggg
    28081 gtttggatac aacctaaaag gtttgcagct ggatatagcg tattcctacc atcaggtgct
    28141 ggggtcaaca ccacacattt ccgtcggcta tgcgttttaa aattgttgcc atactcctgc
    28201 tgataatgcc tgttgcaatg tgttcgtttg cgcaaaacaa cgaaatgcaa tcgctttctg
    28261 atattataga agacatcgcg gagtatagcg acgaagacct tgattatagc agcctgtacg
    28321 agaatttgtt tattttttac aaagaccctc tcgatttgaa taaaaccgat attgaagagc
    28381 ttgaaaaact tcagtttctt actttttttc aggtgaggag cattctgaat tatcgcgata
    28441 aatttgatgg cttcaaatcg atttacgaat tacagtttgt tgatggaatc gaccagcaaa
    28501 cacttgaata tctcctttat tttgttactg ttgcagaaaa gcccggcgca aaggaagtgg
    28561 atatgagtaa atcgcttaag tatggcagac acgttttatt tgtcaggact cagtttactc
    28621 ttcaggaaaa agccggttac ataacggttc ctgacagcgt gcttgctgtt gatcctgaca
    28681 agtcgagata tttgggtagc ccttataaac tgtatacccg ataccgattc aattataagg
    28741 ataaaatgta ttggggtatt acagctgaga aggatgcagg tgaggagttc tttagggggt
    28801 ctcaacccta tggtttcgac tattattcag cccatttcca aataaataag ctgggtccgg
    28861 ttagaaccgc catcatcggg gactatcagg tggaattcgg tcagggactt accttatggt
    28921 cgggtatggg gttcggtaaa agttcaagtc ctaacaacgt cattaaaaaa ggcaggggaa
    28981 taacgcgtta tggctcaacg aatgaaaatg aatttttgcg cggagaagcc gctgcattta
    29041 aaatcggtaa ttttaccatt actgaattct tttcatataa atctattgac gcaagtaccg
    29101 atgccgactc tacctatgat gatgacgaaa ttgtttcgag ttttttgaat acgggatatc
    29161 accgtactcc caatgagatt gcaactaaag acgggattaa ggagctaatc gctggaggaa
    29221 atgttaatta ccgcggcaaa tggcttgcag taggagcaac ggttgcagca gtgaaatatt
    29281 cagccccttt tacgggaaac ggtaggttgt ataatttgtt ttctttttcg ggcacaggta
    29341 tgatgaacgc gggtatcaat tatgtctcga attttggtaa attttcaatt ttcggagaaa
    29401 ctgcaattag cgataatctg gctttggcaa ccctgaatgg gatgagtgta ataatggctc
    29461 ctgaggttag tctgagtgtt ctgcacagat attatgagcc cgattaccat gctatttatg
    29521 cttctccttt ttccgaaggc aataaaccat cgaatgaatc tgggttttat atcggatcta
    29581 ccatttaccc cagaaaacgg tggagaattg acctctatgc tgatttatgg aagtatccat
    29641 ggctgcgtta tggtgttgat gctccttctg atggtcagga atactttgca cagatcaact
    29701 attctcctta ccggaatgta gatatgtttt tcagggcgaa atacgaaacg aaacaggaga
    29761 acaacccgat tacagaatat ggagttcagg atatcattga ttattcatca cttaaactac
    29821 gctatcagct atccgtttct cccggatatg gattccaact gaaaacacgt ttggaatgga
    29881 accggtacca acaagatgtt gctgaacctg aaaacggatt tttgctgtgt caggatgtgg
    29941 gttatacttt cagaaagttc ccgctcgatg ttacggcaag agtagcggtt tttaactccg
    30001 aaacttataa cgcaagattt tacgtctggg agcctgatgt attgtatgcg ttttcaatac
    30061 ccgtatatta cgataaaggg aatcagctct tattattgct aaaatatagt tttggggaac
    30121 acttagattg ctggtttaaa atagccaaca ctttctactg gaataaaacc agtgtaagtt
    30181 cagggcttga agaaattgag gggaacaaga aaacagaagt caaatttcag atacgataca
    30241 atttttaata tgttgattta ttcggcaaag gagcatgaaa aattccttag ggagatcgta
    30301 attgaaccgc ccggaaaaat gcttgaagcg ggttttacgt atcatgctgt tattcttatg
    30361 gcgcaaagta tcgagatttt tggagcctac ttcgataata agccttttcg atctattggt
    30421 cagtcgttaa accgttttgc tcttggtatt gacaacttgt ttccctatgc ttatcacagg
    30481 gctaatgcta gaaatcaatt atacaagcaa ttacgtgcat tgtttgtgca cagttttttg
    30541 cccggaacca atttacagat aaaagaacag ggaaggactg aagagcattt ggagcagaaa
    30601 aacgctacca taataattat ccccgcagta ctgcacaacg atgtgcttca tgcggggata
    30661 aaactgataa aaaaactgaa tgacaatcag ataaacccta aaaaagtatc aaccagtctt
    30721 tttagttgaa gcctccaagt ttctgaatat ctgtgcggat gttaatgcac atcacaggaa
    30781 tctttttgta aagatcctta ttttcatgct ttttcaggtt tttaatgtat tcacggtatt
    30841 tatttgtcat cataagcacc aggtctgcct ctacctctct gatgtaatcg taaattgcat
    30901 cggggtaatg cgaccctaca tccacacgcc tcatttcata gtcaatcagg tctttttcaa
    30961 atatcttctc tgcaaaacgt aaattgttat tcagggtttt aatacgaaat gtatcggaaa
    31021 ccgtagggta aataacaaac gctttgcttt cgaacaaagt gctcagataa cgtacccatt
    31081 ggaattttgc ccgcgacttt tttgtagcat ccatgggaat acaaatgcgg tcgtattctc
    31141 cgaagataac cggactttga accaaaataa atgggatttt aacaaatttt ttgaccaatt
    31201 ttatggcttt atcaagcgat ttgatcccat gagttcccat cacacccaaa tatgcattgt
    31261 tttcgagccc gaatttgtaa atggctttaa acaagttgcc tgtaatgacg gcagtattca
    31321 ctttaatatc tttccctgcc gcaaattgtt ctgctatttc ctgcaattgt ttcagcaact
    31381 caggaacttt agaagatttg ctggcaacat gaaccagata aattggccgc ttcgatgatt
    31441 tgccaatagc ataggcatgc tctagtgcat aattggaaat ttcagtgaaa tcccatggta
    31501 ctaaaatgca tttttccata tattttaaaa ttattggtta gctaaaaata gaataacgta
    31561 aaattttgaa aaaggttgcg tttagaagcc taaaaatgta tttattataa aaattattca
    31621 tgtttacgaa tatctttaat cataagttga aggaatgttt gtcccataaa atgattttca
    31681 aaaatgtgat agcaaacatc aaaaggttta aaatcaaaaa catcggagta tttttctccc
    31741 aaactaaaac caatcccttc tatacataaa tttgcgccta gttcctgaac aacttttacc
    31801 ttgaggtgct cttcctgttt tccaactttt ttcaagctac cattatcata cacatgagtt
    31861 gaaagaaaca ggggcatcat gttattgggc ccgaatggag caaactgatt cagaattctg
    31921 taaaacttcg gagttatatc ttttattttg agctcggcat caatttcaat ggttggtatt
    31981 aattgatctg cttttatggt agtagcaacg taattttcga aacggtcacg gaattcttca
    32041 aaattttccg gtttgaggct taacccggcg gcatacatat gtccgccgaa attttccagt
    32101 aaatcgctgc aagattccac ggccgaatag aggttatatc cagttaccga tcgcgccgac
    32161 ccggtaatca taccattcga caaggtcatt acaatagtag gacggtagta ggtttcggta
    32221 aggcgcgaag caacaatccc gattacacct ttgtgccagt ctttcgaaaa caccacggtg
    32281 gttttttttc tttcagaatc agggtcagat gcaatcattc tcagagcttc ttcagtaatt
    32341 ttctgatcga gttcttttcg gtttgtattg aagttattaa tgtcgttacc gattttgtcg
    32401 gcaaaatgat cgagctcggc taccaacaag tcaaccgcca tgcttcccga ttccattctg
    32461 cctgcagcgt ttatgcgtgg accgagtttg aaaactatat catgtatctg aatatgtttg
    32521 ttattcaatc cactgatatt gataatcgat tttaatcctt tgcacggatc agaattcaac
    32581 ttttgtaaac cataataggc aagaactctg ttttcgcctg taataggaac aatatcggaa
    32641 ccgatactta caaccaccaa atcaagaaaa ctgtaaagct cctgaatatt caaaccgttg
    32701 tgaatgatat atgcctgcat gaacttaaac ccgacgccgc atcccgaaag ctccttgtac
    32761 ggataagggc aatcgctcct tttggggtcc aaaaccgcta cggcacaggg caattcttcg
    32821 ccagggttgt gatgatcgca aataattaca tcaatacctt taccttgcgc ataccgggcc
    32881 ttttcagttg ctttaattcc gcaatcgagt gtgataataa gattgtaatt ttcttcagca
    32941 gcatgatcta ttccctgaat agatactcca taaccttcgc tgtagcggtc gggcacataa
    33001 tagccgatac ggttgtgata ttttctcaaa tagctgtata caagcgcaac tgcagttgtg
    33061 ccgtcaacat cgtaatcgcc gtaaatgagt atcttttcgt tttttttgat tgcttcgttc
    33121 agtctttcaa ccgctttatc catatctttc ataagaaacg gatcgtgcaa atcgctgagc
    33181 tctggtctga aaaataccct ggcagcatcg taggtttcaa tacctctttg tatgagcaat
    33241 tctgcaattg tggcttctac attgagttct ttcattagcc ttaaaacctt ctcctcagcc
    33301 gctttctctt taagaaccca ttttttatcc atgctcgtac caactttaac gtaagataca
    33361 aaattaataa aatttcggtg ctttttattt atttttttga gaataacata aatgagagta
    33421 ttatttataa aactatattt gtcgcacaca aaattgcatg gttatgaaca taattggatt
    33481 gagcgagcag gaaatagtaa gacgcaacgc actcaacgaa cttaggagtt tgaacattaa
    33541 tccttaccct gctgcggagt ataaagttaa tatcagtgcc ggtactattc tgagcacttt
    33601 ttctgatgag aatcccgaag aattgaggga tgtttctttg gcaggacgta ttatgtcgcg
    33661 ccgtattatg ggaagcgcat cttttgctga gttgcaggac gacaccggcc gtattcagtt
    33721 gtatttcaac cgtgatgcaa tttgcccgag tgaggataaa actatgtata acaccgtatt
    33781 caaacgctta cttgatattg gcgatatcat cggagtaaaa ggctatgttt tccgcacaaa
    33841 aatgggagag atcagtgttc atgtcgaaga gttcacattg ctttgcaaat cggtgatgcc
    33901 cttacccatt gtaaaagaga aagatggtca ggttttcgac gcatttaccg atcccgacct
    33961 gcgttaccgg aaccgctcgg ttgacatgat tgtgaaccct catgtgaaag aggtgtttcg
    34021 taaaagaact ttgcttacca ataccatgcg taattttctg aacagctttg gttttctcga
    34081 agtggaaacg cctgtgttgc agccagttta cggtggtgca gcagcccgcc cgttcaagac
    34141 ccaccacaat accctcgatc agaccttata cctccgcatt gctaatgaac tgtatctcaa
    34201 acgcctcatt gttggcggtt tcgatggtgt gtatgaattc tctaaagatt ttcgaaatga
    34261 gggaatggat aggtttcata accctgaatt cactcagatg gaattgtatg tggcttataa
    34321 agactacgaa tggatgatga atctcgttga agagatggtc gaaaatattg ctatggcctt
    34381 gcacggtacg cccgaattga cagttggaga taataccata agcttcaaac gcccgtggaa
    34441 acgctacact atgttcgaag caatacagca ttttactggt gttgatattt ctaaaatgga
    34501 tgaaaatgag ttacggaaaa cagccatttc actgggagct gaaataaacg gaagcatggg
    34561 gaaaggcaaa ctggtagatg caatttttgg agaatgctgt gagtcgaaac ttattcagcc
    34621 tacatttatt acggattatc cggtagaaat gtcgccattg gcaaaaaaac accgttcgaa
    34681 agagggtttg gtagaacgct tcgaagccat ttgcaacggc aaggaaattt gcaatgctta
    34741 ttcggagttg aacgacccga tcgatcagcg cgaacggttc gagcagcagt tgcttctggc
    34801 aaaacggggc gatgaggagg caatgatgct cgatgaagat tttctgaagg ctatcgaagt
    34861 aggaatgcca cctactgcag gtcttggaat tggaatcgac cgcctgtgta tgatcatgac
    34921 caattcggta tcaatccagg atgtgatatt ttttccacaa atgcgccccg aacggaaggt
    34981 tgtggtcgat gatactgatg cattcatagc gttgggaatt ccggaagaat ggatacaaat
    35041 agtacagaaa tcaggttttg cttcggttga tttgctgaaa caggcaaacc caaacaaatt
    35101 gcatgctcag ctgtgtggtt ggaataaaaa gcataaactt ggactaaaga accctacgcc
    35161 tgacgaagtt aaagcatgga tatcgaaata aaccaacaat gccggaacaa aacaccggca
    35221 ttttttttat atttacaaaa aaatctcaaa tcaaatgaaa attgaatacc ggtttctcga
    35281 gccttttgtt tcaaccgagc agataaggga atatacacaa cgggcagttg tcgccctgca
    35341 aaccataaaa aaacgtaacg gtgctggaaa tgattttttg ggatggctcg atctccccat
    35401 gcagtataat ccttcttttg caggtgaaat tgcctccgtt gttaacgatt taaagcatct
    35461 cgaaacggtg gttgttgttg gaatcggtgg ttcctatctt ggagccaggg cagtgatcga
    35521 tgccttagcg aaacctttcg aaaagcagca acccgagatc atttatgccg gaaatcacct
    35581 gagccagcca tatcatgaag cattactccg gtatcttgaa acccgctctt ttggtattat
    35641 cgttatttcg aaatcgggga ctaccaccga acctgccatt gcttttcggc tgcttcgcag
    35701 tttgcttatc agtaaagaag gtcttgaaaa agcatcccgc cacatcgtgg caatcaccga
    35761 taaaagcaaa ggagcactga gaatgatggc tgaccgtgaa cattacaaaa cttttgttat
    35821 agaggataat gttggggggc gatattctgt actgtcgccg gtagggctaa ttcctattgc
    35881 actcgcaggt atttcgaccg acgaactcat ggccggagca agtgcagcta ccacagatac
    35941 aatgctcgaa aatgttgtaa atcctgcagt gcaatatgct gccattcgga attgtttgct
    36001 cgattcagga aaaactaccg aaatcatggt aacctacgaa ccatgcctgc aatacattgc
    36061 cgagtggtgg aagcaattat atggcgaaag tgaaggcaaa cagcacaaag gtttgttccc
    36121 tgcttcagta cagttcacaa ccgacctcca ctcaatgggt cagtacattc aggacggaaa
    36181 gcgaatgatg ttcgaaactg tgctttctgt cgcaaaaact caaggttcac tggttattcc
    36241 tcacgatgat aacgattttg ataagctgaa ttatctggca gataagagcc tcagttatgt
    36301 gaatgacatt gcacgaatgg caacggctca ggcgcatacc gacggtggag tacccgtact
    36361 ctgcatcgaa ttgcccgacc tgaatgcata ccacgttgga tatttgctat atatgttaga
    36421 agtggcgtgt gccatcagtg gctacatgct gggggtgaac cctttcgatc agccgggtgt
    36481 tgaagcttat aagaaaaaca tgttccggat gctgggaaaa gaataaagaa tcatttattg
    36541 ttatacaacc gaataaaact tacatgataa atcgttgatc atcgcaaata tttaatatat
    36601 ttccggcaac taataccatt tatcaattgt ctttgtaagt acaaatggtg aaattataat
    36661 ttatgaaaaa cagaatttta ttgatgttac tttttgtatg cagcttttat agcatactgg
    36721 cgcaacaatc gtgtagtgaa gcagtagaaa tcagttttct ggaatattca acctgtggac
    36781 agattgctat aacaagtgct gatctggctg atgctgcacc ttctgaggat tatccgagtc
    36841 cggagtgtgg taattttagt acgacaacaa acgacttgtg gtataaaatt gtaactccat
    36901 tggatgtgaa cgaactggct tttcatgtta ttaatccggg gatgctttcc gacccatata
    36961 ctttttttcg gcctgctctt gcggtatatt caggctcacc aggaaatctt gagctactgc
    37021 aatgtttcta tgatgaagga ggacctttta gtaatggcga aatccgtttt tctctgataa
    37081 ccaacctaac gccgggacaa acaatttatt tgagagtctg ggatatgaat aataacccct
    37141 ttccgttttt tatcgctgct tcagtgcgaa ccgaaattca ggaatacaat tgcaacacac
    37201 ctgaaattct atcggatggc ggatgtaata ttctggcacc agagggaacg attgatgctc
    37261 cggagaaatg tggatggaat acttccgaca acacatgtta tttcagtttt gttgtgaatc
    37321 cgtcagatcc acaacccgtt actattgggg ctaattatgt tttttgcttc gaaaatggtg
    37381 gaacttacga ataccccgat aacactgtat tgcaaatggc aatctattca tggaatggct
    37441 acgattgcag ttggttgggt ggtagtcccc agtcgatgaa cccaaacgac tctacgtatt
    37501 ggggatgcaa cagtggtacc ggtgttgtta gtatttccgc attgctggat ccaggattat
    37561 acgtgcttgc cttagatggt tatcgtggtc tttcaggtac atctctgtgc acttttgaat
    37621 tctcttcaaa tattgccaat cagtttcaat gccataacga tacaataatt tgctatttca
    37681 gtccaccggt gaatcttgga gaaaacctgc cacagggagg cgtctgggaa ccttcacagt
    37741 ttgtaaacga tggcattttt aatcctgcgg agcccggtga tggtatttgg gagattatct
    37801 attcattggg tgggtacact tgttcatcaa ccatttccgt tgcgagttat cctgttaata
    37861 tttctgatat cgaaatttgt ctggtatcgg ttgatgaaaa caacaaaaac aggattttat
    37921 gggaaaaacc ggatactgat tcacttcagg cgtttcacat atacaaggat aacggtgtag
    37981 cttacgacct gataggcagt gtaccttatg aacatgaaag ttactttgtc gattacaatt
    38041 cagagcccca aacacagtcg ttcagatata aaattgctgg gtatagtgtt tgtgagtttg
    38101 aatcggagct cagcgattat catcagacaa ttctccttaa catggtcgag gaatccggaa
    38161 catggcattt gtcgtggact ccgtacattg gtgcagaggt cggaagcata aatgtgctga
    38221 ggggtaccaa tccgtctgac ctgattacga ttgcaagttt tgatgcagaa acaaccgaat
    38281 tcaccgatac tgatccacct gcaggatttg tttactacca gattgaagtt attcttacgg
    38341 ttcattgcaa tgtagaaaaa tccgtatcag tcgtacgatc gaatatttcg accaatgacc
    38401 ctgattattt caataccggt attggcatgg gcgtacgtga tatttcaaat aaaatttgcc
    38461 ccaatccggc aggtaacagt ttttcccttt gcacagggtc aatggttgag gatgttttca
    38521 tttttaacat acacggtgaa aaggtcaggg atattaggaa ttataaaaac cagataatag
    38581 acgtttctga actcgaaaaa ggaatttact ttgtcaggat tatcggtaac gaagaagttg
    38641 gtatgggcaa acttattata gagtagttgg ctttctagga tatcgaaaaa ttgatttaga
    38701 gcaggcaaac aggtttgatt cattataatt tcataataga aattatagta ctacaaaccc
    38761 gaacgattcg ttatgatcag caaataacat tttggacatc taaacaccgg aatatagcca
    38821 agttcttttg cagatggggt ctattgattt tgcttaataa actttgttcg ttttgtatag
    38881 gtttgagtct taatctcaag aatataaact ccatcttgca attcgctgat attgatgttc
    38941 ggctgtaaat caccgcacat tacacaccgg ccgttcaggt cgaaaatact gaaatatcca
    39001 ttttcataat cagtaagctt gatgttcagc aaattattgg cagggttggg gaatagcctc
    39061 atgttttgag cactattgtt ttccggaatc gaattttcca gcgcttcaac ttgaattcgt
    39121 ttataggctt tgttccctga gctatcgatc accattatat aaaccggata caatcctgct
    39181 gccgatgaag gctgcattgt cagtaatccc gtaggctcta gtgagaaagg tccgtagtag
    39241 gggttccatg taaacaatgt ggattgtatt tcggaagagc aggagcgggg aagcagtttg
    39301 ctgtaatacg tataacagcg cgtctttacg taggaagaat ggaaccagtc ggttacgttg
    39361 cttatgtatc cgaattgtat attcccgttt ttgtaaagca aagttttaaa ataaacttct
    39421 ctgaatttat attgaaaaat agtcaggcag ctatcgctca gacttattct cctctgaaag
    39481 ctttcggaat tcggataata atggcaaaac tccatatcgg aatacgaaat ctgaggctga
    39541 tactcatagg gatatgcttc atggaaccat ggttcttctg tagaaaaata aatttcagcc
    39601 tgacgaccaa ttgaaattgt atcgaaaatt tccccggcaa aattaaccgg ccaaccggca
    39661 gatacctgcg actcacttag gtaaacagaa ttttcatcgg gctctgtatc gtatggtgcg
    39721 tcaattaatt cctgcaaata ttcatcttca cgatacagat aaaaacgata aggagggtag
    39781 ccccctgttg ctgtgagctg ggtttgaaat tctgtattta tttctgtttg caaaccaccg
    39841 gaatactgaa tattcaataa attgctgtcg taatgggagg gaagatcaac gttgaaaccc
    39901 aatttaaaaa cttcttttgc ggcaatcagt gtgtcgtgct gtaatgcagc atactcaaac
    39961 aaagtatcac catcataccg aattattgaa aatgacttga gctggccgtt cacatggctg
    40021 gcgttatcga taataatgaa gatttttgaa attccggggt tttgaatgta tttaagcaat
    40081 gggttgagat cgagcccgag ttcaagagtg tcggcatcag tgagataatc atcaccctgc
    40141 atgggtaaaa gtcctccttg aaaattgaaa ataggaaaat cgataatgtg ttcgggttgt
    40201 aatgattcaa tatcttcgga aatgccaatg ctaattttta ttaaaccccg taatgaatga
    40261 ctcatacgga ttttagccgt aagttcaggt tggtaggtgg tgtccggtac aggaaaatat
    40321 acctttccgt cgtgacaatc ggcaagcata tcgtatttat acagatacaa ccggtaactg
    40381 ccaacataag agtttgccat tacccaaaaa atcttttcgt tgtctctgaa attaacaatg
    40441 ccatcattat tcaaatccag ggtatcggtg attagtccat cctggttaaa atcgtaaaac
    40501 gtgttcgggt agtagccgac cactgtcata ctatggctat acgtgttgcc aatgcattcg
    40561 cttatgttca cgaatccggt gctttgcaat gcggtatcct gaaacgaagg tccgtaatca
    40621 ggtgctccat aattaatggc gatacctccc gaagattctt gtcgcaggtg gtcgtgaaga
    40681 tagtgctgca acagtctgag cccttcatca gtgcttacat cgaggctata atatccggaa
    40741 atacggttct tcattgcacg ataatatttt tcgtaaccgc tcatccaagc cggtatgcga
    40801 ctaccgttag ggctacccgg atgaggaaaa ggttcgggaa agtcgctgag tacagggtgc
    40861 ccctgactca taacaatatc ccatgaatcg aaagaacttg ctccgaaata gttgttgccg
    40921 taattcagaa agaaataggt ataaagggtc gaaaatgtgt tggaagggtc ggcagcctga
    40981 tttttaatac gattgaattc ataggtcatg cagtaataaa ttgccgaagc ctgaccgcaa
    41041 cttggcacat tagcctgatt ataaaattgc tgcgggaaat agggcagctt gtaattcagc
    41101 accttttcgg gaagttcggg cgcatttaag gtataatata tttccgggac aggatagggt
    41161 tctatgtgcg gctgaccatc tgtggtttga gaaaaagaat tgccacaaat cagcaatatg
    41221 agaaacagtg aaatttcaga tgctggtgta aatacgtgct tcatttcggt cgttctaatg
    41281 tattctgttc agccaaatag tattgtgtta aggtagccta ttgattttgc ttaataaact
    41341 ttgttcgttt tgtataggtt tgagtcttaa tctcaagaat ataaactcca tcttgcaatt
    41401 cgctgatagt gatgttcggc tgtaagtcac cgcacattac acaccgacct ttcaggtcga
    41461 aaatactgaa atatgcattt tcataatcag ttaggctgat gctcagcaaa ttattggcag
    41521 ggttaggata aacattgact ttctctgttg gaacaggttc ttcaatgtgt accattttag
    41581 gtgcgtaccg gttttttagt atcgcagtgt tatgaccaac aacaatcagg cttccgacag
    41641 gggtttctgc aatagcgtta agatcttgat aggtaaaccg cggtatggtt tcccaggttt
    41701 caccggcatc aaccgacatt aaaaaagctc cgctctgtcc tgccacatat cctgtgtcgc
    41761 cttcgttaaa catgatgtcg ttgaaccaag cactttgcga agatattccg tcaacaagct
    41821 gccagtttac ccctccgtca taggtttttt taatcaccgg aactgcagtg aattctccgg
    41881 tttggctatt gaagttttct gctatataac cgcaaacaaa tcctgtgtcg ttatttacga
    41941 aagccacaga atacaaactg ccgttatcct caaaaactcc catattctgc catgtgctac
    42001 cgccatctga agtttttata gtggtatgat acgaccctac ggcatagccc acagtatcgt
    42061 ttgtaaagaa tatatcctgc agataactgt cggttgccga gttctgctct gcccagttat
    42121 gccctccgtt ggtggtttta aaaacgtgtc cgttggagcc tgctgcataa ccgattagct
    42181 cattggtaaa gaaaatacta gtaatcagaa aattattcat cagcacaaca gtttcccatg
    42241 tttcaccccc atcatccgaa agcattacct gaccgtagtt ggtgcttatt actatgttgt
    42301 tttccccaag atattccaca tcagtaaaat cgagatcatc gctgaattta tcaagctccc
    42361 aggtgtttcc tccatttgct gtgtgcagta aatttccatc attacctact gctgttccga
    42421 tattgtcgtc gtaaaattca acattgtaaa gcacacaata ttcttcggtg agtttgtcgg
    42481 tcattagtat ccacgatgct ccggagttat tcgaccggta aatttcccct aacatatttg
    42541 caacaaataa agtgcctacc gaagtggcac atattgcatt aatcatctgg ttgttcgtga
    42601 tatcctgcaa ctcccaggtt gttcccccat cactggtaat aaaaatttca ccaaagtcgt
    42661 ttccgaccca gccatgattt gcatcggtga aaaaacattt cctcaaatac gactcggtga
    42721 aattaatgtc ttctcctacg gtttgtgctg tccagttgtt tccaccgtct gtagttttca
    42781 tcatggttcc gagaaaacca caggcatatc ccgtatttgc atcggtgaaa aacagggaac
    42841 tcagaaactc ggaagaacct gtaggtttgg ctgtccagtt tgctcctccg ttggtggttt
    42901 tgtacacata tcctccatcg caacaaacat atccggtact ctggtcaaca aactgcatat
    42961 cccaaatgtt tttatccatg gtactgaatg caacactcca tgagttacca ccatcagtag
    43021 ttctcaggat tttcccgttc cagcaggcga ttacccctag catggaattg aaaaagtgtc
    43081 cggcgacaaa attctggttc tcaaagccgg tttccagtgt tgtccattca ttgcccccat
    43141 cgattgtata gtaaagattc ccttcatttc cgaacgcaaa acctttattt tcattaagaa
    43201 aagatagcga cataatataa tcatcaccga cacttccaat ggaataccac gaaaacccac
    43261 cgtttgtggt tttaatgata tgcccggcac caccggatgc atagcccgtg tgggaatcaa
    43321 caaaaatcat tgcgtatatt gattctccgg tgggagtggg gttttgccat acccactgcc
    43381 cataagaagt aggtattaat agcaacatgc ttccaataaa aagtaaaagc gctctcattg
    43441 ctgaagtttt ctgcaattta accaaataat atttaaaata caaacatgta attaaagcga
    43501 tattttttac aattaaattt aatagagtgg aataatacat tttattgttt ataaaaatat
    43561 taactgtgaa ttacattttc ttttcaaatt actatatttg ttgccgtaat ggtttccgtc
    43621 gggtcaaccg gcggatgaaa agggaacagg gtgaaagacc ctgacagtac ccgctgctgt
    43681 aagctcttta aaaaccgttg ccgaacatgc cactgtcgta aatcaggatc tatacctgag
    43741 atgggaaggc cggtaacgca gagtaagtca gaagacctgc catcgcaaac aatgttttta
    43801 aggctttcgg gtataaaagc caaggacgga aaaatttccc cctgatgccc aaagacttac
    43861 aaacagtaac acgtggtgta atgttatgta gtgtttaatt gaaaaatggt tggaattatg
    43921 aaaaaaaaag tactatttac agcattatta ttgctgtcgg tgggcacaaa tgcagtattg
    43981 cacgcccagc aaaatgtcga ttttgaagaa cttacacttg atcccgaaag ccactggaac
    44041 ggctccgacg ggtcaggcat gtttactaca ggcggatgga attttataaa tgtttacgac
    44101 gatgtttacg gctcgtggat gggctggtca tacaccaacg aaaccgacaa tactacctac
    44161 gactggcaaa atatgtacag tagcgcatct ggagagggat tgtttggttc cgaaaattat
    44221 gcaacggcct atgtaatgag cgattggatg aataacaatg aaccaattcc ttgtgtggtg
    44281 cagcgaagcg ataaccaacc cgttgaaatg ttcggcacct ggatttgcct caatgcgaat
    44341 gcgtcgctat atatggccta cgatgatttc tacagcaatc agaaccatta cctaaagctt
    44401 attgtgaacg gacatatgtc tgaagttctg ccggcaatgg taacgcagga atatattctt
    44461 gcagattacc gcttcgaaaa cgatgaatat gattttaaat atgacaattg gcattttatt
    44521 agtctgatgg gcctcggaag tgcgtataag cttgaattta cacttgtgtc agacgatatg
    44581 ggtgaatatg gcttggtaac cccgggatat ttctgtatag ataatttcaa ttctgaatac
    44641 tttctttcgt atctcccgga tatgtttgtt gaaacgcccg aaattattac catcgacgaa
    44701 ggacaatcgg ttgacctgac agcactggcc ggtgggggaa ttcagcctta cacctttgag
    44761 tggcagtata acgagagcct gagttcgact tcaggtcaaa cggtaacagc cagtcctgct
    44821 gaaacgacca catattatgt tactgtatcg gatgttatgc ccaattcacc cggtcatacc
    44881 gtagaaaaat ccattactgt cgttgtaaat catgtgggta tcgatagcaa acaactgaat
    44941 acggtaaatg ttcacccgaa tccttgtagc gatgtcgtta cggtttctaa tgttcctgct
    45001 ggttctgagg ttcagatata tgacttgcag ggtcgaattg ttacttcagt aatagcgcaa
    45061 agcagctctg ttcagattcc ggtatcgcaa ttggcagcag gtgtgtacca agtaatttca
    45121 atcgcagggg aagttcgcag tattcataaa ctggttatca ggtaggttat gctgaaggtg
    45181 aaaaacataa tcctgatatc atttttgata tcttcctcgt tgcttaaggc acaggtttac
    45241 gacaattatc agaatgcagt gaatgtggac gattcacgca ttgtaggctg ggtaagtcaa
    45301 tgcgaagtag agcgtggtta tattaatatt tccgacccgg agcagaccta tactcagggg
    45361 gatgtgacat cgaaccgtgc atttgccgga acagagcagg atgcctgcgg agctgccaat
    45421 gggttgtcaa cggtcagcct tggcgatggc ggtagcgcaa cagtgcagtt ccagaacccc
    45481 attcgcgatg gcgaaggcta cgattttgtg gttttcgaaa atgggttttt caacccgccc
    45541 ggcgaaacct cagctgcatt tatcgaactg gcatttgtag aggtcagctc cgatggtacc
    45601 cactttttca gatttcaaac cctgtcggag atgccttatc taacgcagaa caacagcttt
    45661 gccgccatcg actggacgtt gtttcatggc ttcgccggtg tgtatgcaca cccctatgga
    45721 gtggctttcg atgtggctga tatttccgat gccccatttc tgaacaagca acgaattacg
    45781 tacattagaa ttgtggatgt ggtgggttgc attcagccgc aatatgcaag ctacgata
//
LOCUS       DAOOQP010000010        42995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1010_length_42995_cov_5.674220, whole genome shotgun sequence.
ACCESSION   DAOOQP010000010 DAOOQP010000000
VERSION     DAOOQP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 42995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_42995_cov_5.674220"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLK75_02060"
     CDS             complement(<1..966)
                     /locus_tag="PLK75_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38061.1"
                     /translation="MKQIPFRDLFSTRNIILLLILIVSFASFIRVGDCNFLFWDDDML
                     ILKNPYVRYLTWESITHNFAQERFTALTLSIYSFMYKLWGNNPAPYHWLNLIIHLANI
                     VLVFKLMQHFFKNIYSVILVSLLFALHPLRVESVAWVSELKGLLCTFFILSSVLFYIK
                     YLQSNRKWFLFALAAFLAILSSFSKIQGIITPLLWLLLDIYFRRKDYSLSIVEKLVML
                     IFPLFTDNISIMATLLLVITVAFLFKDRIDQVKKMYIYIGSIVITLSGIFILFLNFGS
                     SFTNFWNNQPENQIVFSFTERILLAGFSLWFYFRSFFYPFPMSAFH"
     gene            1296..1739
                     /locus_tag="PLK75_02065"
     CDS             1296..1739
                     /locus_tag="PLK75_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38062.1"
                     /translation="MKLKAISASVILIFLITLSLKSEAQSVSEWLPVGLSVSGNNIQK
                     NVEALYGVSVCNGVDVILLKFINNNEYEVRVEWTNGIFTDQGEWVTDPLRTELNSIVI
                     PEGSEVFGDCENAAGSLLLVKISDFLSVPGSLNKYRALEFHVYTD"
     gene            1751..>3872
                     /locus_tag="PLK75_02070"
     CDS             1751..>3872
                     /locus_tag="PLK75_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOY38063.1"
                     /translation="MKNIYKILLSGFFLLIVGYSGVYGQTATTTVYFSGFQACGGCTV
                     CGSDYWCTNTPGSYCGDTPPCKTLSFTDPVPAGHVVTSVTANYYTGSCEGASFTSSIN
                     GFSLPIAYDGATGCWCDDLPCMMTTSVTGNFPCGLPGYIYGGSNSFNLCSSGPMCINR
                     AELIFTYVDPDVITPSITASGPLSFCMPGSVTLDAGTGYSAYHWNTGSTSQTIVATTA
                     GVYTVTVTSTTGCTSGSSSVTVTAAPNVTPTFTQLGPYCKGQIPGVLPTTSLNGITGT
                     WSPSTINTNNFGTTVHTFTPNAGQCAITTTMTVVVSPNTTPTFTQLGPYCAGQTPGTL
                     PTTSTNSVAGTWSPATVNTSTPGTFTHTFTPNSGQCAEIVTMDIVVYANPTANATIHG
                     NPSCNGSSDGSIVLNVSGGTSPYTFSWSNGASSQDLFDIPAGTYSVLVTDAHGCTASS
                     SASLVEPNPIVASVGSVTPSICNTPGSATVSATGGTPPYTFTWPASAGGYVGPTHSSL
                     SSGTYVVTASDQYGCSSSATVTVPQTGSVSASVGSITNVSCYGANNGSVQVNITAGTP
                     DFTISWGSGSISTNLTSYTFTNLPAGTFGLTITDANGCVTSLSGITISEPQALVVQLN
                     SITHVTCNGGSNGSASIGVTGGTPPYSYAWTPSTLSGANPTGLSAGNYSVTVSDAHNC
                     TGTISFTIQETSDFVIVKDRENAPC"
     assembly_gap    3873..3882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3883..5709
                     /locus_tag="PLK75_02075"
     CDS             <3883..5709
                     /locus_tag="PLK75_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY38064.1"
                     /translation="GETGSATISVLSGGTPPYSILWSNGSSQFTNGSIPANTPTGFTV
                     TDSQGCSFADTVIITQPDLLTTSISGTDALCGGSNTGSATITVTGGGTPPYFYHWSNN
                     SVNPTINYLVAGTYSVTVTDFNGCSSVQSITITDPDDLLLSIAGTPIACGVSAGSATA
                     TASGGIPPYTFHWSNGDQGPNATNLSDGIYYCTVTDNNSCTATSSVNIGITGVLNPLI
                     TVVQNITCNGAANGILSADITAAQPPYSFHWSNNSNQPIISNLQPGNYIVTVYDDWGC
                     TGTATGIITEPPAITINAVIQDIRCTGVSDGSISVTVTGGTPDYIVEWSNGSHDYTIS
                     NLSAGTCSVVIKDSNSCQRTQSFVINEPTSGVNVSAAVTDVSCFGGSDGSIAMFATGG
                     TLPYSFSCSNANFEINQQLVANLPAGTYIISVVDDYGCVDDSTVMIIEPSVIIASFVS
                     TDPSCTGNNDGSIDLNASGGTEPYVYNWLGVNYNLPYFNGLLEGEYYFTIIDNRGCEL
                     SLGPIILTDNPINCIRIPNAFSPNNDGVNDTWEIENLHMFPEAVVQVFNRWGQELYNN
                     TIQEGDWDGYYNGKLLPTGVYLYVIVLFNGTDPYTGAVTLVK"
     gene            6009..7004
                     /locus_tag="PLK75_02080"
     CDS             6009..7004
                     /locus_tag="PLK75_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY38065.1"
                     /translation="MTQTVNVQELNELIRQESAFVDIINFEVQKVIVGQKHLLEGLMI
                     GMLSDGHILLEGVPGLAKTLAINTLANSIQAGFRRIQFTPDLLPADLIGTMIYSQKNE
                     EFIVKKGPIFTNFILADEINRAPAKVQSALLEAMQERQVTIGDKTYPLEKPFLVMATM
                     NPIEQEGTYPLPEAQVDRFMLKIVIDYPKFEEERLIIRENLAQDFPKATSVVKPADII
                     KARKLVREVYLDEKIEKYILDIVFATRFPDQYKLEKFKPLIEYGGSPRASINLAMASK
                     SFAFIKRRGFVIPEDVRAVCHDVLRHRIGLTYEAEAENITTEEIISEVLNTVEVP"
     gene            7079..7942
                     /locus_tag="PLK75_02085"
     CDS             7079..7942
                     /locus_tag="PLK75_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOY38066.1"
                     /translation="METAELLKRVRRIEIKTKGLSNQIFAGEYHSAFKGKGMAFSEVR
                     EYMYGDPIKHIDWHVTARFNHPYVKVFEEEREMTAILLIDVSGSGQFGTHTQLKQYLA
                     AEIAAVISFSVIQNNDKIGVVFFSDNVEKFIPPKKGKKHILHIIRELLNFEPQNKQTN
                     ISEALRFLTNAIKKRSTTFIISDFISPDYSDALKIASRKHDVVALRLFDRREAELTDI
                     GLVQVCDNETNEIKWIDTSSARVRKQYNSWWLRHDKICRETLTKCGVDFVSIATGDDY
                     IKPLISLFKNR"
     gene            7945..9342
                     /locus_tag="PLK75_02090"
     CDS             7945..9342
                     /locus_tag="PLK75_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38067.1"
                     /translation="MTRFPLIILIAIVSLWGISQAVAQQASVLTRLSADSLIIGEQIQ
                     FTIQYSGDNPDDVVFPVYNDTLTKGIFIVKQGDIIEGKTGSQPLFSRDYVITAFDTGT
                     CYIPVVKLQVVFGTDTLELFSDSIKLLVKPYLLIDTVPRDTVFAENFGIVVFGKNNFA
                     NEISMAIPDSIARSVTEDQLKAMVDSVREQYIQQFASELYRTIGFRNDEDVKIISAGS
                     DKTIFIVDNNSIVETHRIPGANDTVFIHQYDTVRKGQALFVTLKIMDISDELFKTPLT
                     WAEFWWRVKMFLKHNWWWIALSLAAMALILYYFVFRKKGIKNIFRRIPPPEPPHIVAL
                     RELNRIRDEKKWQNGKTKEYYTELTDVLRKYFEGRFGVNAMEMTSDEILNAMSGFSVI
                     TETYMNQLKQVLITADAVKFAKAEPFFHENDMHLKNAFDIVEGTVEEQQPDKVKIQEA
                     YIEVESEKGEESKNV"
     gene            9335..10330
                     /locus_tag="PLK75_02095"
     CDS             9335..10330
                     /locus_tag="PLK75_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY38068.1"
                     /translation="MFNDIRFLHIEYFWLLSAIIPMSIWYVYRIRKSHPSLNISSSVA
                     FSGMASGLKVRMRHIMFVLRMVAFAAAVAVLARPQSENAWQTSTTEGIDIMLAIDISG
                     SMLAEDLKPNRLEAAKDVAIGFINARQNDRIGLVIFGGESFTQCPLTTDKSVLVNLFK
                     EIRNDLLEDGTAIGSGLATSVSRLKESDAKSKVIILLTDGVNNSGAVAPVTAAEIAKT
                     FGVRVYTIGVGRKGTAPYPVQTAFGTQYRNMEVKIDEDVLKEIAKTTGGQYFRAEDNE
                     MLKKIYQDIDQLEKTKLQISDFSKPEEKFFWFAVIALAALLCELILRFTIYKPMP"
     gene            10343..11374
                     /locus_tag="PLK75_02100"
     CDS             10343..11374
                     /locus_tag="PLK75_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY38069.1"
                     /translation="MFQFHSSIYLWLLWIVPVLVALWILSRYIRKKQLQRFGDNEIIK
                     KLMPAVSTRFRILRNVVLLLAITMLVLALARPRFGSSLLEVKRNGIEMIVAIDVSNSM
                     LTRDVQPNRLERTKKSVERLIADLNNDKIGLIVFAGDSYMQLPVTNDYASARMFLQTI
                     NPSLAPKQGTAIGSAIDLAINSFSSDNQTGKVLVIFTDGENHEDDPIASAKAADEKGI
                     VIHTVGMGLPKGQPVPSPDGNGFMKDKNGNVVMSKLDESTLKSIAATAHGIYVRTSET
                     NSGLDLISDEINKMQQTKIASQKYADYDEKFTYPAFLAMLLLVIELFIPGKKSLKTRR
                     IDLFNVDKK"
     gene            11377..12057
                     /locus_tag="PLK75_02105"
     CDS             11377..12057
                     /locus_tag="PLK75_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38070.1"
                     /translation="MTKWIFYIVFAIISINSVYAQAERKHIREGNKHYEKKEYQDAEI
                     AYKKATDLQPEMYQIRGNMGAASFRQQKYEDASEAFTGYMLAARSVSEQAKAWYNLGT
                     ARLQEGDYVKSIDAFQKALILNPKDDDSRYNLSIAQYMLSKQRQQGGGTGSEQNQQQQ
                     NKPKEQQNGDNNENKQKQNKQSDPEKISKGDAERILKALEQDEKDLQKKVQEQKVKTQ
                     KINVEKDW"
     gene            12140..12640
                     /locus_tag="PLK75_02110"
     CDS             12140..12640
                     /locus_tag="PLK75_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HOY38071.1"
                     /translation="MMQNNPKPVTLIEHTGTVSPDIFRRMHRDFTNILDHLEFAISSQ
                     KKVFSIFDELLTNVQKHSWHEHDYEIEFRVELLEKSITVHSVNPVEPLQLVPLKKRVD
                     ALSQCDATHLKAGYFSTISNETPLGNGAGLGLITVFRKSSAVITSFSTTGKGVPIFFM
                     QAVIHL"
     gene            12647..14446
                     /gene="uvrC"
                     /locus_tag="PLK75_02115"
     CDS             12647..14446
                     /gene="uvrC"
                     /locus_tag="PLK75_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOY38072.1"
                     /translation="MVTNEIKNEIASLPAKPGVYKFIDSSGTIIYIGKAKNLKKRVSS
                     YFNKTQENNKTRILVNKIAGIQHTVVDNESEALLLENNLIKQWQPRYNLLLKDDKTYP
                     WICITGELFPRIFYTRTKEGAKNEYFGPYASVHTVKTLLQLIRQLYKIRTCRLLLNQD
                     SIQNGRFQVCLEYHIGNCNAPCVGLQTEKAYSEDIKLARDIIKGNLQRVTDYLKNQML
                     DFAKDYKFEEANSIKQKIAIIESYKAKSTVVSNKIKRIDVYGLAEDKDSVYISFLKIV
                     DGAVVHSHNAEMRAKFGETKQELLVSYAFEMWSNSGEKAPEAVVPFLPEFKIEGLTWT
                     VPRQGDKFKLLELAGRNAKIYMLERHRIIEKKDPEIAIERKLKALQLDLHLREIPDYF
                     ECFDNSNFQGSNPVASCVVFRNAKPSKKEYRHFNIKTVEGPDDFASMQEIVFRRYKRI
                     MDQNEELPKLIIVDGGKGQLSAAVEILKKLGVYGKLAVIGIAKRLEEIYFPEDSVPAY
                     LDKNSESLRLIRFARDEAHRFAVSFHRKKRSSEFLISEFEQIQGIGEKSILLLIDKFK
                     SLSEVKKANQEDIISLVGKSKAEKIASYFAMKK"
     gene            complement(14473..17526)
                     /gene="secDF"
                     /locus_tag="PLK75_02120"
     CDS             complement(14473..17526)
                     /gene="secDF"
                     /locus_tag="PLK75_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOY38073.1"
                     /translation="MRNKGAIILVAVLLTLICVYYLSFTVRTYQIENRASKYAEAKYS
                     GMKLKLDDLEKSHKLDSIKKHYLDSLTKSGESVLWMFKKYTYNEIKERQLNLGLDLKG
                     GMNVTIEVSAADIVRALSNNRSDTVLNKALQIAVDKQVSSNESFVDIFAKAYKEVSKG
                     KSLANIFITSGNAKISISSTDAQVITYLKEEYTAAIGNANEVLRKRIDHFGVVQPNIQ
                     EDFAVKGRFHIELPGIKDPERVRKLLQGTALLEFWETFENKEVYKSLEKIDEYLAKRG
                     QPDTTNVEAATDTVQNTAPVPNQNVIAGDDVTETPDTNAAVKPATAANDKKESDKALA
                     KSQHPLFVILNPSASKDGQLMEGPVVGRAVATDTAEINAIFRDEKVKAFLNKDLVLAW
                     SFKPLENSDDVFELIALRRVKNGAVLNGDVITNAREDFGQTSAYAEVSMTMNADGSNE
                     WARITRDNKERCIAIVLDGYVYSYPRVQNEIKGGRSSITGNFTINEAKDLANVLKSGK
                     MPAPANILSEEIVGPSLGQKAIDSGLNSFIIAFIIVMLYMVVYYGRPGLTANLALLAN
                     LFFIFGVLASFGAVLTLPGIAGIVLTIGMAVDANILIFERIREEMRAGKALSLAVKDG
                     YKNAYSAIIDGNLTTIIVAIILFNFGVGPIKGFATTLIIGILSSLFSAIFITRLVFDT
                     KIKRNKKIIFETPISKNAFQNVKIKFIEKRKIAYVISGILIAISIISLVTRGLNPGID
                     FTGGRTYIVKFDNVVNPQEVAKNLKLTFTEAPEVKTFGAGSQVKITTKYLISSSDKTT
                     DAKIDSLLYVGLKPMLPTNISQQEFLASYRQSSQKVGPAVAQDVLSKSFIAVLISLLG
                     IFIYILFRFKKWQYSLGAVASLVHDSLIIIGAFSLFYSIAPFSMEVDQAFIAAILTVI
                     GYSINDTVVVFDRIREYIREFPKRNLKETMNQAINSTLSRTMGTSLTTLLVLLAIFIF
                     GGEVIRGFVFAILLGIFVGTYSSIFVATPIAYQFMKKKQKDAETK"
     gene            complement(17602..18063)
                     /locus_tag="PLK75_02125"
     CDS             complement(17602..18063)
                     /locus_tag="PLK75_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOY38074.1"
                     /translation="MKQLKKNQIKVFSVPLIICELDKQGIHLFAQCRIGRKKRIVLID
                     TGASQSVFDKNSEVFSDNEFNKITEENASSGINSSISEMYVSTIKSFALGGFVLRNYF
                     SVFIPFDHINSMYHTIGKHKITGIIGGDILLKYRAEISYLDKELRLHLNIE"
     gene            18186..18719
                     /locus_tag="PLK75_02130"
     CDS             18186..18719
                     /locus_tag="PLK75_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOY38075.1"
                     /translation="MRNKIILLSIAVMMITGVSMAQKADCNNSKKEQATLYGIQDLTP
                     EQEKAIQTIKKDMHSVQKNLKAERDIKEAELNKLLIQDKADLKAIDAKIDEITAIRAQ
                     MEKNRVHANMNIRQLLNEDQKMQFDHKTMGQNKARCAEGEGQMPENKPCGHNDHQNSG
                     EKAPGNHKECPHSQDKK"
     gene            18843..19634
                     /locus_tag="PLK75_02135"
     CDS             18843..19634
                     /locus_tag="PLK75_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38076.1"
                     /translation="MKTIWVFYVLSLIVVPSYSQWVFQKEIPFSLGNVGVDILGNLYV
                     FDSYQIKKTDSNGKVIFSYANKNLGEITGADVTDPMRIMLFYGDFNYLVFLDNSLSLL
                     RDPVSLDGLNLNRAEVVCASQKGGFWVFDSGDLRLKYFDSNLKQLNTSSAIDVDVSND
                     KSISLKEFTDYVCLVFEKNGCFVFDKFGNFVFRIQEQGFSAPYVNNGYLYYILKNELV
                     EHNLKLHNETKIALPVDNSIQVLFNGSLLYVRQGDKLQIYFLVEN"
     gene            19692..20306
                     /locus_tag="PLK75_02140"
     CDS             19692..20306
                     /locus_tag="PLK75_02140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOY38077.1"
                     /translation="MHIAIAGNIGSGKTTLTRLLAKHYSWTPHFEDVEDNPYLTDFYD
                     DMQRWSFNLQIYFLNSRFNQILDIRKSGKTVIQDRTIYEDAYIFAPNLHAMGLMTSRD
                     FDNYFSLFNLMSSLVMPPDLLIYLRASVPTLVQQIQQRGRKYENTIRLDYLQRLNERY
                     EAWINGYKLGKLLIVDVDHTDFTAVQEDLRDVINKIDAEIHGLF"
     gene            complement(20935..21927)
                     /locus_tag="PLK75_02145"
     CDS             complement(20935..21927)
                     /locus_tag="PLK75_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOY38078.1"
                     /translation="MKKILAMGNALVDMMTQLPNDSILSDLALPKGSMQLVDATQSKQ
                     IQEFTKSMVRTRVSGGSAANTIRSIANLKAPCGYLGKVSNDELGIFFRNEMNALGINT
                     LLQHSATPTGLAVALVSPDSERTFATYLGAAAELTPEELSKESMAGFDYLHIEGYLAF
                     NHSLIKKAMEIARANNIIVSMDMASYNVVEINRDFLLDVIKNDVDIVFANEEEARALT
                     GMGPEDAVHQIAEMCRIAIVKTGADGSLIKSQGQLSKIPARKTSPVDTTGAGDNYASG
                     FLYGHANGWSLKKCGQLGTVMASRVIEVIGTTMSSETWEWINAEKECIAASGND"
     gene            21993..22066
                     /locus_tag="PLK75_02150"
     tRNA            21993..22066
                     /locus_tag="PLK75_02150"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22027..22029,aa:Asn,seq:gtt)
     gene            complement(22225..22581)
                     /locus_tag="PLK75_02155"
     CDS             complement(22225..22581)
                     /locus_tag="PLK75_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38079.1"
                     /translation="MKNAALLILVFALFACSGSPRKPQSNNTDTTKTSEVKPVNEYTV
                     DMYNKIEVGMNLADVKNILLKDGVLLTQASSTANQQGAGKISAKLYTWTNKDGSYIEI
                     TFVEGIVVTKNKVNLN"
     gene            complement(22703..23704)
                     /locus_tag="PLK75_02160"
     CDS             complement(22703..23704)
                     /locus_tag="PLK75_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOY38080.1"
                     /translation="MVTISVALVLVVLELSFIFTGYKSTYLEKRYKLYYASHYAAEDK
                     NWFHVWHRNHYLESSEYRYYRSINSLGLSDIEHPVEKDSSEYRIIALGDSFTEGDGAD
                     ADSTWLKFLERGLQKYNYPLHLRFMNAGVCGSDPYFEYILLREKLLQYKPDLVIVCIN
                     STDILDVMLRGDMARFQPDGSVKYNHAPWFEPIYASSHLSRIVFEALGYDEFLTGDRN
                     SGDLSTRTEKQIIQCLYDFKKLADENGFRLLVAFQPLSWEVVNNSSSLQKVINATKQA
                     HIDYCDLMEYYTVVEKMDSATVLNYFWSKDGHHNSTGYGAFGRGIEKHILLMELFSK"
     gene            complement(23971..24852)
                     /locus_tag="PLK75_02165"
     CDS             complement(23971..24852)
                     /locus_tag="PLK75_02165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOY38081.1"
                     /translation="MGRLFFTLKIIILSPIVIVFTGILLTVICCRTPKTVSVITEYSP
                     EQKALWQKQKGSINEINRLFAENNQDYFTDIQRDTIKPDNIFRIAVIGDSWIWGDGIY
                     YKYTWSHLLEDKILEKYKNTELISWGLCGWSTLHEYRYFINNGFRYHSDLLIVGVVEN
                     DPSLSYDDQPAEFKKWQEGLYSDFNLKGYENLILDFVDSVNSNGTEILFVLTPLSADS
                     TRMRWNNKIVDIMTRNNLNYLNLYPAFANKFKGLPPEVLFASPVNGHPGLRLNKFFAN
                     EVFNYLKRNNYLIKKVS"
     gene            25022..26209
                     /locus_tag="PLK75_02170"
     CDS             25022..26209
                     /locus_tag="PLK75_02170"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOY38082.1"
                     /translation="MEATYSRIKALSEKYSDYTAKNLSELVKIKSLSLQEKDVQLALK
                     RQMEEAGFDEVFIDGLGNVIGRIGNGKKVLAIDGHMDTVDIGNTANWSFDPLGGEIKN
                     GFVHGRGTVDQEGGPAAFVTAGRVLKELGFNRDVTVYFVGSVMEEDCDGLCWKYIIEE
                     DKIKPDFVISTEPTNLNIYRGHRGRMEMHVHFYGVSSHGSAPERGKNAIYMASRVALE
                     IEKLNEKLAYDEFLGRGSVTISEIISGSPSLCAVADYARVHLDRRLTWGETKESAVKE
                     IEALVQGMNAKVEVLNYYETAYTGLKYGMEKYYPTWKMPEDHEIVVTGVKAYEKLFEK
                     KPTIDKWTFSTNGIMTNGVYGIPTIGFGPGNEVLAHAPDEKVAVSDLVVAAAFYAAFA
                     FEM"
     gene            26229..27419
                     /gene="ygeW"
                     /locus_tag="PLK75_02175"
     CDS             26229..27419
                     /gene="ygeW"
                     /locus_tag="PLK75_02175"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOY38083.1"
                     /translation="MKLIEKIEALRLLKSGLYQKDFLLTWEKTEEEIRLILEVASIMK
                     EMRSQNISLRVFDSGLAISNFRDNSTRTRFSFASASNMLGLAVQDLDEEKSQIAHGET
                     VRETANMISFMSDFIGIRDDIFLGEGNRYMREVGAALDEGFSQGVLPVRPGIVNLQCD
                     IDHPTQAMADLMHLKHYFGSLDKLKGKKIVMSWAYSPSYGKPLSVPQGIIGLMSRFGM
                     DIVLAHPEGYGLIPNVVERAKTQAQQSGGSFSVMHSMDEAMKGADIVYPKSWAPYHIM
                     QQRTELLKNADKNGLKELEQVCLANNAVYKNWEYDATKMKSTKNEDALYMHCLPADIS
                     GVSCKEGEVNGDVFEKYRIRTYIEAGFKPYIIAAMMFTNRFENPAEALRKLVERNRLR
                     VGAL"
     gene            complement(27432..28808)
                     /gene="hydA"
                     /locus_tag="PLK75_02180"
     CDS             complement(27432..28808)
                     /gene="hydA"
                     /locus_tag="PLK75_02180"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOY38084.1"
                     /translation="MDILIKNGNIITSEYSVVNDVYIHEGKIHSIGKNLKHSATTRIV
                     DASGKFVFPGGVDPHVHMCLPTPAGPSSDDFRTGSIAALYGGTTTLIDFVTPVKGESL
                     PNALEKRKREAENSLIDYSFHVSPIEWHSGTRNEISDCFSNGITSFKVYLAYRKTVGL
                     CDADFLKVLRTVGELGGIVTAHCELGEEIERLRDYYFSVNKTEPLYHYLSRPAEMESD
                     SVKKAIALAQEAGCSFYVVHVSTKKSLFHIEEAKKKGQQVYAETCPHYLLLNNPPITG
                     SFASAAPYIISPPLREPCDNEALWHAINKNVVSTIGTDHCPFNMEQKRAGANDFRSIP
                     NGAGSIEYRLPLLYTYGVLGKKISINKMVDIFSTMPAKIFGLFPQKGCIAIGSDADIV
                     IWNPDGSHSISLLNQHQHCDINIYNGLSVSGHAEYIVKGGEIAVNNDILFSSISRGRF
                     LKRNRPEL"
     gene            complement(28914..30410)
                     /locus_tag="PLK75_02185"
     CDS             complement(28914..30410)
                     /locus_tag="PLK75_02185"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY38085.1"
                     /translation="MRSVIISFICILTAALLSSCYKEEVIYDLQPDNSLELSTILRFN
                     GKDCVFDSGRNLLRYPVNADSIVHFEPFTEFQDYSEIYFANRQLSRNTINNIGTVHIN
                     QEYPIIVKTGDFTKEITLVFTNLPIVQITTISGFFDEPKTLARLTIHYPEPERPSYSS
                     YIGIEYRGVTAQLFPKKSYGFAILGSMNIKDRRSCQLFSMRLLENWILDAMYIDKGRL
                     RNKVSFEIWRNMGENTQHDGLQSELVELVINNSHQGLYCLNENITAELLQFSQPDAVL
                     YKAYDWANGAVTFDIPPEMPSTIQYWDGWEQKFPDPELKTNWMPLYKLRDFVVNSSDA
                     EFCDSIADLIDMDNFIDYFLFLNLVTALDNVGKNTFLAQTGNTDKFFIIPWDIDASWG
                     LFWDGTYIGYTNRLTNNLYNRLINLNPDNFRERLKTRWVSLRNSNFTETEILSLFERD
                     FEKISKSDIISLENSIWSLNLNIEEERNFIQTWIPARLNYLDAYISGL"
     gene            complement(30415..31095)
                     /locus_tag="PLK75_02190"
     CDS             complement(30415..31095)
                     /locus_tag="PLK75_02190"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOY38086.1"
                     /translation="MKHIALFSFALLISSLLISQEIAVVRDAGLWAGIGVGKRITKDF
                     QAEFNQELRLYHNCTKIDDCISDLGIDYSINRNFSIGANGRYTYNIKRDEVIENDLRY
                     GFDMNYKARLGLRFKLFCRARYSKEYFDAFNKISQEKLTETDFRYRLKLEYQYNGMHS
                     LYTSGEIFRTTDKLRDPYFSKIRCFAGDEIRIQSGVLNLAIGYELQNKISYRTSFVFM
                     AISYTLKL"
     gene            complement(31235..33427)
                     /locus_tag="PLK75_02195"
     CDS             complement(31235..33427)
                     /locus_tag="PLK75_02195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOY38087.1"
                     /translation="MESYLFWIVPIASVLALGFAYFFYRQMMRADEGTELMKTIASHV
                     RKGAMAYLKQQYKVVLIVFAVISVLFAFLAYGLEIQNPFVPFAFLTGGFFSGLAGFFG
                     MKTATYASARAANAARKSLDSGLKLAFRSGAVMGLVVVGLGLLDISIWFYILQLVYPP
                     SADPNNLLIITTTTLTFGMGASTQALFARVGGGIFTKAADVGADLVGKVEAGIPEDDP
                     RNPATIADNVGDNVGDVAGMGADLYESYCGAILSTAALGAAGFIYLGAETAYKAVIAP
                     MLIAAVGIVLSIIGIFIVRTKEGASQKKLLRALGNGVNASAIMIAIASYFILRLLELP
                     NYAGIWWSMIVGLIAGIGIGQATEYFTSSSYKPTRRISAASKTGHATVIIAGIGTGMI
                     STIFPVLIVGAAIMLAFGFSANWEMTNFGMGLYGIAIAAVGMLSTLGITLATDAYGPI
                     ADNAGGNAEMSGLEHEVRKRTDALDTLGNTTAATGKGFAIGSAALTALALLASFIEEV
                     RQGLIHFHDNPVLPNLNENVKASEAGMIDIIQSFEVHLMNPKVLVGAFIGAVAAFLFC
                     GLTMNAVGRAAQKMVDEVRRQFREIAGILEGKATPDYARCVSISTKAAQREMMFPALI
                     AISIPILVGIVFGVAGVMGLLLGSISSGFILAVFMANAGGAWDNAKKYIEEGNFGGKG
                     SDCHKAAVTGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGVTLAFSF"
     gene            complement(33559..34338)
                     /locus_tag="PLK75_02200"
     CDS             complement(33559..34338)
                     /locus_tag="PLK75_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOY38088.1"
                     /translation="MLSKAQIARIKSLADKKNRDIEGLFVAEGTKIIKDALESGVNIK
                     YLIATDDWIKSNESGLAVTNLEITPVSSETMKKISSFRSPAQVLAVFEKPAKSDPPLH
                     EAEITLALDGIQDPGNFGTIIRLCEWFGITTIICSESTVDAYNPKVIQSSMGACLRVN
                     IYYSDLSLYIKRYKHQTKNPVYGTFLSGNPLGVSKLSQQCLIILGNEGNGISQRLEIL
                     IDTKINIPAYHSPNMQMESLNVAVAAGIICYEFRKRGLPLL"
     gene            complement(34345..35406)
                     /locus_tag="PLK75_02205"
     CDS             complement(34345..35406)
                     /locus_tag="PLK75_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOY38089.1"
                     /translation="MSRFNDYFNFSRREKRGLIVLCAIIILLISSQAIMHRISFNKRY
                     DFSAFESEIDAFTRRNQNSPNDSALELFYFNPNTIDENGLIKLGINKNTAHNIINYRN
                     KGGKFRKPADFARMYGLGPEQFEKLEPYLVFENSVQSSREQAPQVNYFYFDPNTASEE
                     ELMSLGIKKYMAANIVKYRSKGGRFKEPSDLLSIYSFDSACYNNLEPWIKIEANQNKP
                     IENLHIELNTATAEDLIKIKGIGNAYASRIISFREKLGGFAHVEQLREVYGMTEELFT
                     SVKPHVYADASKIDCINLNDCDFKTLISHPYLEKTDVIAILNYRTMVVRINSTEELLK
                     QKVISSETYTRIKDYFCTN"
     gene            35714..36169
                     /locus_tag="PLK75_02210"
     CDS             35714..36169
                     /locus_tag="PLK75_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOY38090.1"
                     /translation="MDKIRLDAMDKRILELLIKNSKTPVVDIAKKCKISAAAIHKRIN
                     KLEEVGVIGGYRLVVNPQILDFKTQAFIGIILERANMFAGVTEKMREIPEVVELHFTT
                     GQYGILAKAYCRDNKHLQDVLVKIHNITGVSNTDTMISIEQLLDRSIPL"
     gene            complement(36173..36964)
                     /locus_tag="PLK75_02215"
     CDS             complement(36173..36964)
                     /locus_tag="PLK75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOY38091.1"
                     /translation="MRKLLLNNKFIFGLIALNAVVIFALSYDAIHEKAAIFLTATDIL
                     LTLIFVAEILVKIREFGINKFFKSTWNVFDFLIISISFISVFFYFIDIEAADLSYVIV
                     IRVARVFKTFLFIRFIPNINGLLGGIRRAFRASLLVLISLFVYNFILGIFSCHIYKNI
                     LPECFGDPITSIYSIFRIFTIEGWYEIPNAIAANTSPAIGVISTIFFVGILISGGVLG
                     ISLLNSIFVDAMVADNTKELEAKVDELNQKISKLSDQIEKLSQKI"
     gene            37048..38067
                     /locus_tag="PLK75_02220"
     CDS             37048..38067
                     /locus_tag="PLK75_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOY38092.1"
                     /translation="MSAIIKNRIEYIDVIRGIAIFLMIAANSAPYIMNEPYNFAFRVF
                     SSLAAPLFIALSGYMLGLHSTNLKTWRTVYKGGLIVLTGVLVDVFIWDLMPFVSYDVL
                     YLIGICVMLYPLISLMNRVQNIAWIVAVFVVSFISQFVFNYQIRNTAFQFDWNNLGIV
                     FHQSKFLLFDGWFPIFPWAGIFLLGHFAGKYTILLKRKQKLYVLIISIAVLLFAVFLL
                     FLQNRPTREGYCELFYPADVFYCLTAVSLLVIFAIIYKIFNTRIWEPLRWLGRSSLFF
                     YVLHLIIIRFFIEKLHLLFGEHLFITFIVFYIFTFIVGFLLYKLKKTGVWQKLPFILR
                     FLLGS"
     gene            38022..38984
                     /locus_tag="PLK75_02225"
     CDS             38022..38984
                     /locus_tag="PLK75_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38093.1"
                     /translation="MAKAPLYIAILAGILICFSPGRLYAQKDTVKVGVFINSLHDFNF
                     YDKSYKIDFWLWLLYSNAELSFDEAIEIRPSKEFDLSNTILDSTAGLQWLSMKVRAEI
                     VYHWQLDHYPFDNQMLNVVIESADADTSSMVFIPDFENSKIDPNFKLDEWDIDTVIVN
                     NAISTYETTYGDQELTGQSSYPAINIAVYLNRKNSWLSLFKLITGVVVAFFISCLVFL
                     IKPTNVDPRFGLCVGGLFAAVGNKYIIEGTVPSTNTITMLDNIHNLTYVLILLIILVS
                     IFSLKLFEKGTEKSIRQSQRVDRISLFVIIGIYLLAMAYLIVVR"
     gene            39006..39740
                     /locus_tag="PLK75_02230"
     CDS             39006..39740
                     /locus_tag="PLK75_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38094.1"
                     /translation="MRKIVLIVGVLISLMLWCSNSSAQFIQHNYIEVGKTNASGGIYL
                     DAASLSAIRYKHIHAELGFLFPLTEFDKFLSTFYASAGVDFKIKNLPVEIHALFTDKL
                     VSEFISELQFGIIAGVKTKHVHVFLGNNTRLFNLTRNAIEQSRNDNYRKLQIVEKLNL
                     MYSLSYLVFPQENKWNIIASVLNYDYFLIHQETNPFVSVRFTYNPRENIGLYSEIVYK
                     SAGFNNIQVNYFGVNLLTGILWEIDF"
     gene            39722..40582
                     /locus_tag="PLK75_02235"
     CDS             39722..40582
                     /locus_tag="PLK75_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOY38095.1"
                     /translation="MGNRFLKIGLFAVFFTGALLMSSCIGDADFTGFFRSSVRVNERF
                     EQSLEWNTQHAPRRIVCENNNYRLFFASDIHIGGTENFQKILNDSKSAETDGLFLVGD
                     IVSGKEEDYNILVESLARESIENYFIQVGNHELYFNGWKWFRQYFGTSVYPVEIVTPL
                     AKDLVICLDSGGGTLGPLQLEWLEELLRNVRQNYRHCIVLHHCNFFQSDFSTSATPLS
                     EELYELMNLFFTYSVDMVIMGHVHISSEENFGPTRYICLDAALDGAKNASYLRLDCSD
                     DHLVYQYISL"
     gene            complement(40607..41179)
                     /locus_tag="PLK75_02240"
     CDS             complement(40607..41179)
                     /locus_tag="PLK75_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38096.1"
                     /translation="MSRTKTIIKITFIFSALISCGGITQPMKEVTGPESVSIEPAADK
                     TVLDSSCNDNIVYLKYYNDRFKYSINYPDFLTPLGEPDNNDGQAFRSNSGEMELLVFG
                     NNLTKGTFQEGYEDIVKVGFYADPEYLINFSHFEGNRYEIRGKNRKDSFYLTSAFYGS
                     FSVTVFITVPHSKEAEHASIIRNIQQSVNY"
     gene            complement(41203..41973)
                     /gene="rsmA"
                     /locus_tag="PLK75_02245"
     CDS             complement(41203..41973)
                     /gene="rsmA"
                     /locus_tag="PLK75_02245"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOY38097.1"
                     /translation="MTDIRAKKRLGQHFLIDKNIARKITDSLLNREALPIIEVGAGTG
                     VLSEYLSEKNVPVTLIDIDPEAVAVLKQKYSHKEIKIILGDFLKLDFKQNFEPKVCII
                     GNFPYNISSQIFFNLIANRDSIDEVVCMVQKEVAQRIAAKSGSKTYGILSVLLQTWFD
                     IEYLFTVNETVFVPPPKVKSAVIRLRRNNTILLGCDESAFIKVVKAAFNQRRKTLRNA
                     LKTLGKDSREIPDEILDNRAEKLEISQFVLITDILFAS"
     gene            42073..>42995
                     /locus_tag="PLK75_02250"
     CDS             42073..>42995
                     /locus_tag="PLK75_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOY38098.1"
                     /translation="MNKTLKNSIQFVLFLGFGVLLFWLVYRDTNWSDLVAGLKNVNYW
                     WFIPIVVAGLFSHVSRAIRWNMMIEPLGYKTRFWNTFFAVMTGYFANLAIPRLGEVAR
                     CGVVSKYDKVPISKVLGTMITERVIDVLSLLLVVVIAIFVESGFFSDFLQQNPGIGEK
                     VDSLLNPLPIIIAIAAVALFIVLFFLILKGKFDKIRLFLKLKTLARNLYDGLLSVRKV
                     RKKFWFLFHSIFIWILYYLMMAFAFPAFGFTSHIGPSAALMVFVAGSFGMIAPAPNGM
                     GAYHFMVIQSLLIFGIVKEDAAVFAFVVHGL"
BASE COUNT        12949 a         8260 c         8799 g        12977 t
ORIGIN      
        1 atgaaaagca gacataggaa aggggtagaa aaaactgcgg aaataaaacc acagcgaaaa
       61 ccccgccagt aatattcgtt ctgtaaaact gaaaacaatt tgattttccg gttgattgtt
      121 ccagaaatta gtaaagctgc tcccaaaatt taaaaataga ataaatattc ctgaaagggt
      181 tataacaatt gatcctatat aaatatacat ctttttaact tgatcaatcc tgtctttaaa
      241 cagaaatgcg actgtaataa caagaagcag ggtcgccata atgcttatgt tatctgtaaa
      301 gagaggaaaa atcagcatta caagtttttc aacgatggac agggagtaat ctttccttcg
      361 gaagtaaata tcaagcagca gccataaaag cggtgtgatt attccctgaa tttttgaaaa
      421 agaagataga atagcaagaa aagctgccaa tgcaaataaa aaccactttc ggttgctttg
      481 caggtatttt atgtaaaaca acacggaaga caatatgaag aaagtgcaaa gtagcccttt
      541 gagctccgac acccatgcaa ccgactcaac cctgaggggg tgcaaggcaa acagcaagct
      601 aaccaatata acggaatata tgtttttaaa gaaatgctgc attaatttga aaaccagcac
      661 aatgttggca aggtgtatta tcaagtttag ccagtgataa ggcgcaggat tattgcccca
      721 tagcttatac atgaatgagt atatgctgag ggtcaaagct gtaaaccttt cctgtgcaaa
      781 gttatgagta atgctttccc aggttagata ccttacatac ggatttttta atatcagcat
      841 atcgtcatcc cagaataaaa aattgcagtc acctacccga atgaacgatg caaaactgac
      901 aatcagaatt aggagcagaa ttatattccg ggttgaaaaa agatctctga acggaatttg
      961 tttcattata aagcaatgtc ttcaaagcaa ttataacaaa tataaaataa tacttttaca
     1021 tatttgaaca gaaacctgtt tttatacgaa gtttttataa ataaatttgg tgcttgctta
     1081 aatgttgacg cagttattag tataaatagt atgcttagaa tagctttaaa tcacgtaata
     1141 agtatttaaa ataaataaat ataaatataa tgaattgtag ttttgatatc ttgaaaaacc
     1201 gaattaatga ttcatgtttt catgaatttc tgaaaaaatt tgtaaattgt tacgatttta
     1261 aatagtatat ttgttataat tcaatttttg caaacatgaa attaaaagcc attagtgcat
     1321 cagttatcct gatttttctg ataactctgt ctttaaaaag tgaagcgcag tcggttagtg
     1381 aatggcttcc tgtaggcttg tcggtaagcg gtaataatat tcagaaaaac gttgaagcat
     1441 tgtatggagt atctgtttgc aatggcgtag atgtgattct actgaaattt attaacaata
     1501 atgaatacga agttagggtt gaatggacca acggaatttt tacagaccag ggagaatggg
     1561 tcaccgaccc attgcgaact gaattgaact ccattgttat tcctgagggt tctgaggttt
     1621 ttggtgattg cgaaaatgct gcaggaagcc ttttacttgt taaaatttct gattttttat
     1681 cggtgcccgg tagtttaaat aaatatcgtg cacttgagtt tcatgtctat actgattaat
     1741 tactcggcct atgaaaaata tttacaagat tcttttatcg ggcttttttc tgctgatagt
     1801 cggctattcg ggcgtttatg gacaaacagc caccaccact gtatatttct cgggttttca
     1861 ggcttgcgga ggatgtacgg tttgcggttc cgactactgg tgcactaaca cacccggaag
     1921 ttattgtggt gataccccac catgcaaaac tcttagcttt accgaccctg ttccggcagg
     1981 tcacgtggta accagtgtta cggctaacta ttatacggga agttgtgaag gagcatcatt
     2041 tacctcctcc atcaacggtt tttcgctacc cattgcttat gatggagcta ccggatgctg
     2101 gtgcgatgat ttaccttgta tgatgactac ttcagttaca ggaaatttcc cctgtgggct
     2161 gccgggttat atttatggag gcagtaattc gtttaatctt tgttcaagcg gcccaatgtg
     2221 catcaacaga gctgaactta tttttactta tgttgatccg gatgttatta ctccttccat
     2281 tactgcttca ggaccactgt ctttctgtat gcccggaagt gttactctcg atgcgggaac
     2341 aggatattca gcatatcact ggaataccgg gtcaacttct caaaccattg ttgcaactac
     2401 tgctggggtg tacactgtta ctgtgaccag taccacaggg tgtaccagcg gttcaagttc
     2461 tgttactgtg accgcggctc ccaatgttac accaacattt actcaactgg gtccgtattg
     2521 taaagggcaa attcccggcg tattacccac tacatcgctg aatggtatta ccggcacatg
     2581 gagtccttct accatcaata caaataattt tggaactact gtacatactt ttacacccaa
     2641 tgccggtcaa tgtgcaatca caactactat gaccgtggtt gttagcccga atacaacacc
     2701 cacctttacg cagttgggtc cctattgtgc cgggcaaact cccggtactt taccgacaac
     2761 ttcaaccaat agtgttgctg gaacatggtc gcctgccacg gtaaatactt caactccggg
     2821 gacattcacc catacattta cccctaactc cggacagtgt gctgagatcg ttactatgga
     2881 tattgttgtg tatgcaaacc cgacggcgaa tgcaactatt cacgggaacc cttcttgcaa
     2941 cggtagctcc gatggcagca ttgtgctgaa tgtaagcgga ggcacctctc cgtacacatt
     3001 ttcatggagc aacggggcat cttcgcaaga tctgttcgac atccctgctg gtacctactc
     3061 ggttctggtt accgatgcgc acggttgtac tgcgtcatct tctgcaagtc tggttgaacc
     3121 caatcctatt gttgcttcgg taggatcagt tactccaagc atttgtaata ctccaggaag
     3181 tgccaccgtt tcagcaacgg gtggaacgcc cccctataca tttacttggc cagcaagtgc
     3241 gggtggttat gtaggcccaa cacattccag tctatcatca ggaacctatg tagttacagc
     3301 tagcgaccaa tatggctgta gcagcagtgc aacagttacc gttcctcaaa ccgggagtgt
     3361 cagtgccagt gtgggcagca ttacgaacgt aagttgttat ggtgcaaata atggcagtgt
     3421 tcaggtgaat attacagcgg gtacacccga tttcactata agctggggaa gtggaagcat
     3481 cagtacaaat ctcacatcat atactttcac gaacctgcct gctggaacct ttggacttac
     3541 aataaccgat gcaaatggtt gtgtaacgag tttaagcgga attacaattt ccgaaccaca
     3601 ggctttggtg gttcaattaa actctattac gcatgttacc tgcaatggtg gcagcaacgg
     3661 atcagcaagt attggggtaa cgggtggaac tcctccgtat agttatgcat ggactccatc
     3721 tacgctctcg ggtgcgaacc ctacaggact ttctgctgga aattacagtg taactgttag
     3781 cgatgctcac aattgcacag gtaccatttc ttttacaatt caggaaacca gcgactttgt
     3841 tattgttaaa gacagagaaa atgcaccctg ctnnnnnnnn nnggtgaaac tggttctgct
     3901 acaatttctg tactaagcgg gggcactccc ccgtattcca ttctctggtc taatggttca
     3961 tcgcagttta ccaatggaag tattccggca aatacaccga ccggattcac agtaaccgat
     4021 tctcagggat gctcttttgc tgacacagtg attattacac agcccgattt gctcaccact
     4081 tcaatcagtg gaaccgatgc attatgtggt ggttcgaata ccggaagtgc tacaattact
     4141 gtaacaggtg gaggtacgcc accatatttt tatcattggt cgaacaacag cgtaaaccct
     4201 acaatcaatt atcttgtggc tggaacatac tcagtaacag taaccgactt taacggttgt
     4261 agttcagttc agtcaattac tataaccgac cccgacgatc ttctgctatc aattgccgga
     4321 acgccaattg cttgtggtgt aagcgccgga agtgcaacag cgactgcatc aggaggtatt
     4381 cctccatata ctttccattg gtcgaatggt gatcagggtc caaatgctac caatctctcc
     4441 gatggcatat actattgtac agttaccgat aataattcat gcaccgctac ctcatcagta
     4501 aatatcggta tcacaggggt attaaatccg ctgataaccg ttgtgcagaa tatcacatgc
     4561 aatggggctg ctaatggaat ccttagtgct gatattacag ctgcccagcc gccctactct
     4621 ttccattggt cgaacaattc aaatcaacca ataatctcaa accttcagcc tggaaattat
     4681 attgtaaccg tttacgacga ttggggttgt acaggcacag caacgggtat tattacagaa
     4741 ccgcctgcca taaccattaa tgctgtaata caggatatcc ggtgtaccgg agtttccgat
     4801 gggtctattt ctgttacagt taccggagga acccctgatt atatagttga atggtcgaat
     4861 ggttctcacg actatacaat tagcaattta tcagccggaa cgtgttctgt tgttataaaa
     4921 gatagtaata gttgccagcg aacacaatcg tttgtaataa atgaaccgac ctcaggggtc
     4981 aatgtcagtg cagctgttac cgacgtaagt tgttttggag gcagcgacgg gagtattgct
     5041 atgtttgcca ctggaggaac actgccttac tctttctctt gtagtaatgc aaacttcgaa
     5101 ataaatcaac agttggttgc caatctgcct gccggcacct atattatctc tgttgtcgat
     5161 gactatggtt gtgtggatga ttccactgta atgataattg aaccatcggt aattattgct
     5221 tcatttgttt ctaccgatcc ttcctgcaca gggaataatg atggctccat cgacctgaat
     5281 gcttcgggag gcactgaacc ctatgtctac aactggttgg gtgtaaatta taacctgcca
     5341 tatttcaatg gtttactgga gggagaatat tattttacca ttatagataa ccgaggatgc
     5401 gagttgtcgt tgggaccaat aatattaact gataatccaa tcaattgtat cagaatcccg
     5461 aatgcttttt cgccgaataa cgacggtgtg aacgatacct gggaaataga aaacctgcat
     5521 atgttccctg aagcagttgt tcaggtattt aaccgctggg ggcaggagtt gtataacaac
     5581 acgattcagg aaggtgattg ggatggctat tacaatggaa aacttcttcc taccggagtt
     5641 tacctgtatg taattgtttt gtttaacgga accgacccat atacaggagc tgttacactg
     5701 gtgaaataga agtacgctga tagagttggc tatatttacc ctaggttaga aatccccgat
     5761 cggcttctaa ggaatgcagt cttataatta ttggtcacca ataccggaaa atgcaatcgc
     5821 tgagctttgg gttagcatca ataatcaaat aatttcatgt atttcattag ttgtcaaaaa
     5881 cgcctttaac agagttttaa cagcttttaa caataatata agcattacca cagcatcttg
     5941 gttgtattat ttcgttttac tttataattt cgtgattcaa tttaataaac actttaatat
     6001 tcatatatat gacacagacc gtgaatgtac aggaattgaa cgaactgatc cgacaggaaa
     6061 gtgcatttgt cgatatcatt aattttgagg ttcaaaaggt aattgttggg caaaagcacc
     6121 tgctcgaagg attgatgatt ggtatgctca gcgatggtca cattcttctg gaaggtgtgc
     6181 caggtttggc aaagacactg gcaatcaata cacttgctaa tagtattcag gccgggttcc
     6241 gccgtattca gtttaccccc gacctgttgc ctgccgatct tatagggact atgatatata
     6301 gtcagaagaa cgaagaattc attgtgaaga aaggtccgat ttttacaaat tttatccttg
     6361 ccgatgaaat taacagggct cccgctaaag tgcagtcagc attgctcgaa gccatgcagg
     6421 agcggcaggt tactattggt gataaaacct atcccctcga aaagccattt cttgttatgg
     6481 ctacaatgaa tcccattgaa caggaaggta cgtatccgct tcctgaagct caggtggatc
     6541 ggtttatgct taaaatcgtg attgattacc caaaatttga ggaggagcga ctgataattc
     6601 gggaaaacct cgctcaggat ttcccgaaag caacaagcgt agtaaagcct gctgatatta
     6661 tcaaagcacg gaaactggta cgggaagtat atcttgatga gaaaattgaa aaatatattc
     6721 tcgatattgt gtttgcaaca cggttccccg atcagtacaa attagagaaa ttcaaacccc
     6781 taattgaata cggaggctcg ccccgtgcaa gcataaatct tgccatggcc tcaaagtcat
     6841 ttgcttttat taaaagacgc gggtttgtga taccggaaga tgtgcgtgcg gtttgtcatg
     6901 atgttctgcg gcatagaatt ggccttactt acgaagctga agccgaaaat attacaacgg
     6961 aagagattat cagcgaagta ttaaatacag ttgaagtgcc atagaaatgg atgtaaattt
     7021 gagaaaatcg gtatgataac gggttgggta attgtatcca taatgaaaga gatactcaat
     7081 ggagacagca gaattattaa aacgggtaag aagaattgag ataaaaacaa agggtttgtc
     7141 gaatcaaatc tttgcagggg agtaccacag tgcttttaaa ggaaaaggta tggcctttag
     7201 cgaagttcgt gaatatatgt acggagaccc catcaagcat atcgactggc atgttaccgc
     7261 ccgattcaac cacccttatg taaaagtgtt tgaagaggaa cgcgagatga ctgcaatttt
     7321 gcttatcgat gttagtggtt ccgggcagtt tggaacccat actcaactga agcaatatct
     7381 ggccgcagaa attgcagctg ttatttcttt ctctgtaatt caaaacaacg acaaaattgg
     7441 tgtggttttt tttagcgata acgttgaaaa attcatacca cccaaaaaag gtaagaaaca
     7501 cattttgcat attatcaggg aattactgaa ttttgagccg caaaacaagc agacgaatat
     7561 ttcagaagca ttgcgatttc ttaccaatgc aattaaaaaa cgaagcacaa cctttatcat
     7621 ttccgacttt atttctcctg attactccga tgccttaaaa attgcatcac gcaaacacga
     7681 tgttgttgcg ctccgcttgt ttgatcgacg agaagcagag cttaccgata ttggtctggt
     7741 gcaggtatgc gataacgaaa ccaatgaaat aaaatggatt gatacttctt cagccagagt
     7801 aaggaaacag tataatagct ggtggttaag gcatgataaa atctgccgcg aaactcttac
     7861 caagtgcggt gtcgattttg taagtattgc aacgggcgac gattatatta aaccattaat
     7921 ttcactgttc aaaaacaggt aaagatgact cgatttccgc tcataattct aatagcgatt
     7981 gtttcccttt ggggaataag ccaagccgtt gcacagcagg ccagtgtgtt gacgcgacta
     8041 tctgctgaca gcctgattat cggagaacag attcaattca ctattcagta ttcaggagat
     8101 aatcccgatg atgttgtttt tccggtttat aacgatacac tcacaaaagg aatattcatt
     8161 gtgaaacaag gcgatataat tgaaggtaaa accggatctc agccactatt tagccgcgat
     8221 tatgtcatta cagctttcga tacaggaact tgctatattc cggtggtgaa acttcaggtt
     8281 gtttttggaa ccgacacgct tgaattattt tccgacagta taaaactgct tgtgaaacca
     8341 tacctgctta tcgacacagt cccaagagat actgtgtttg cggaaaattt cggaattgtg
     8401 gttttcggaa aaaataactt cgcaaacgaa atttcgatgg ctattcccga ctcgattgcc
     8461 aggtcggtaa ccgaagatca actgaaagca atggttgatt ctgtgcgcga acaatatatc
     8521 cagcaatttg cttctgaatt gtatcgcaca attggtttca gaaatgatga agatgtaaaa
     8581 ataatcagtg ccggttccga caaaactatt tttattgttg ataataacag tattgttgaa
     8641 acccatcgta ttccgggagc caatgacaca gtttttattc atcagtacga tactgtgcgt
     8701 aaagggcaag cattgttcgt tacgctgaaa attatggata tcagcgatga gcttttcaaa
     8761 accccactta cctgggctga gttctggtgg agagtgaaaa tgtttctgaa acataactgg
     8821 tggtggattg cattgtcgct ggcagcaatg gcattaatac tatattattt tgttttcaga
     8881 aagaaaggta ttaaaaatat tttcagaagg attccacctc cagagcctcc tcatatcgtg
     8941 gcattgcgcg aattaaacag aatacgcgat gaaaagaaat ggcaaaacgg caaaaccaag
     9001 gaatactaca ccgaactgac agatgtgttg cgaaaatact ttgaaggcag atttggggta
     9061 aatgctatgg aaatgacctc cgatgaaatt ttaaatgcaa tgtcgggatt ttcagtaatt
     9121 actgaaactt acatgaacca gttaaaacaa gttctcatta cggctgatgc ggtgaaattt
     9181 gcaaaagcag agcctttctt ccatgaaaac gacatgcatc ttaaaaatgc ttttgatatt
     9241 gttgaaggta cagttgaaga gcagcaaccc gataaggtaa agattcagga ggcatatatt
     9301 gaagtagaat ctgaaaaagg ggaggaatca aaaaatgttt aacgatattc gttttttaca
     9361 tattgaatac ttttggcttt tgtcggcaat tataccaatg agcatttggt acgtgtatcg
     9421 tatccggaaa agccatccaa gcctgaacat ttcatcttcg gtggcttttt caggaatggc
     9481 ttctggatta aaagtgcgga tgcgacatat aatgtttgtg ttgcggatgg ttgcctttgc
     9541 tgcagcagtt gctgtgttgg cacgacctca aagcgaaaac gcttggcaaa caagcactac
     9601 tgagggtatt gatattatgc ttgctattga tataagcgga agtatgctgg cagaagattt
     9661 aaaacccaac cggttggaag cagcaaagga tgtggctatt ggctttatta atgcacgcca
     9721 aaacgacaga ataggtctgg ttatttttgg aggagaaagc tttacacagt gtccacttac
     9781 aaccgataaa tcggttcttg taaacttatt taaagaaata agaaacgacc ttttagaaga
     9841 tggtacagcc attggttcag ggttggctac atcagtaagc agactgaaag aaagcgatgc
     9901 aaaaagtaaa gttattattc ttcttaccga tggggtaaac aatagcgggg ctgttgctcc
     9961 tgttaccgct gccgaaatag caaaaacatt cggcgttcgg gtttatacaa ttggtgtagg
    10021 acgaaaaggc accgctccat accctgttca aaccgccttt ggaacgcagt atcgcaatat
    10081 ggaagtcaaa attgatgaag atgtgttgaa agaaattgcc aagaccacgg gtggtcagta
    10141 ttttagggct gaagacaacg aaatgcttaa gaaaatttat caagatattg atcaactcga
    10201 aaaaacgaag ttacagataa gtgattttag taaacctgaa gaaaagtttt tctggttcgc
    10261 tgtaattgcc cttgctgcat tactatgtga gctgatattg cgttttacaa tttacaaacc
    10321 aatgccataa ggagagaata ttatgtttca gtttcatagc agcatatatt tatggttatt
    10381 gtggatagtg ccagtacttg tagcattatg gatactttca cgctatatca ggaaaaagca
    10441 attgcagcgc tttggtgaca atgaaattat aaaaaagctc atgccggcag tgtcgacaag
    10501 atttcgcatt ctacgcaatg tggtcttact tctggcaatt accatgttgg tgctcgcttt
    10561 ggcacggcct cggtttggaa gtagtctgct cgaggtgaaa aggaatggta tagagatgat
    10621 tgtagccatc gacgtttcga atagtatgct taccagagat gttcagccta accggctcga
    10681 gcgcacaaaa aaatcggtgg agcgtttgat tgctgattta aataatgata aaataggcct
    10741 aattgttttt gcaggtgatt cttatatgca attgcctgtt accaacgatt atgcatcagc
    10801 cagaatgttt cttcagacca ttaatccttc gctggcacca aagcaaggta cagcaatagg
    10861 ctcagccatc gaccttgcaa ttaattcgtt tagctccgat aaccaaaccg gtaaggtact
    10921 tgttattttt actgatggcg aaaatcatga agacgacccc attgcttcag caaaagctgc
    10981 cgatgaaaag ggtattgtaa ttcacaccgt tggaatgggt ctgccaaaag ggcaacctgt
    11041 gccctctccc gatgggaatg gttttatgaa agacaaaaac ggaaacgtgg ttatgtcgaa
    11101 actcgatgag agtacactta aaagcattgc tgctactgca catggaattt atgtgcgaac
    11161 cagtgagacc aattccgggc tcgatttaat ttccgatgaa ataaacaaaa tgcaacagac
    11221 aaaaattgct tctcaaaaat atgccgatta tgatgagaag tttacttatc ctgcttttct
    11281 ggctatgttg ctgcttgtga ttgaactatt tattcctgga aagaaaagct taaaaacaag
    11341 gaggattgat ttgtttaatg ttgataaaaa atgaaaatga ctaagtggat tttttatatt
    11401 gtatttgcca ttatatcaat aaactctgtt tatgcacagg cagagcgaaa gcatatcaga
    11461 gaaggaaaca aacattacga gaaaaaggaa tatcaggatg ccgaaattgc ttacaaaaaa
    11521 gcgacagacc tgcaacctga aatgtatcag ataaggggaa atatgggtgc tgcttcattc
    11581 cgccagcaaa aatacgaaga tgcatcagag gcttttacag gttatatgct tgctgcaagg
    11641 tctgtcagtg agcaggcaaa ggcatggtat aatcttggta ctgcaaggtt gcaggagggc
    11701 gattacgtaa aaagcatcga tgcctttcag aaagcactga ttttaaaccc aaaggatgat
    11761 gacagccgat acaatttatc tattgcacag tatatgcttt cgaaacagcg gcaacaaggg
    11821 ggtgggaccg gctctgaaca aaatcagcag caacaaaaca aaccaaaaga gcagcaaaac
    11881 ggcgataata acgaaaataa acagaaacaa aacaaacaat ctgaccctga aaaaatttcg
    11941 aaaggggatg ccgaacggat tttaaaggct ttggaacaag acgaaaaaga cctccagaaa
    12001 aaagttcagg aacaaaaagt gaaaactcag aaaattaatg ttgaaaaaga ctggtaagca
    12061 acttttactt tgctgatttg cagctttctt cgattttagt aaatttacat tctctctttt
    12121 attgagacga aatgtattaa tgatgcagaa taatccaaaa cctgttaccc tcatagaaca
    12181 taccggaacc gttagtcccg atattttcag acggatgcat cgagacttta caaacattct
    12241 ggaccatttg gagtttgcta tctcttcaca gaaaaaggtg ttttcaattt ttgatgaatt
    12301 attaacgaat gtccaaaaac actcctggca tgaacatgat tatgaaattg agttcagggt
    12361 agagttattg gagaaatcaa tcactgttca ttctgtcaat cccgttgagc ctttacaact
    12421 ggttccgctt aaaaagaggg ttgatgcact ttcgcagtgc gacgcaaccc atctgaaagc
    12481 aggatacttc tcaaccattt caaacgaaac tcctttgggt aatggtgcag gtctgggctt
    12541 gataactgtt ttccgcaaaa gcagtgctgt tattacctct ttcagtacaa ccggcaaggg
    12601 tgtgccgatt ttttttatgc aagcagttat tcatttgtaa ttgaagatgg ttacgaacga
    12661 gattaaaaac gagatagcat cgcttccggc aaaacccgga gtgtacaagt ttatcgattc
    12721 ttcagggacc ataatttata ttggtaaagc taaaaatctg aaaaaaagag ttagttccta
    12781 tttcaataaa acacaggaaa ataacaaaac gcgtatcctc gtcaacaaga ttgccggtat
    12841 acaacacact gtggtcgata acgaaagtga agcactactc cttgaaaata atcttatcaa
    12901 acagtggcaa ccccggtata atttgttact gaaggacgat aagacctatc catggatttg
    12961 tattacaggg gagttgtttc caaggatatt ctatacacgc accaaagaag gagcaaaaaa
    13021 cgaatatttt ggcccgtatg catcggtaca tactgttaag acattgttgc agctgataag
    13081 gcagttatat aagatcagaa cctgcaggtt attgttgaat caggattcca ttcagaacgg
    13141 taggtttcag gtatgccttg agtaccatat tggtaattgc aatgcacctt gtgtgggact
    13201 ccaaacggaa aaagcatata gcgaagatat taaacttgcc agagatatta ttaagggaaa
    13261 cctgcaacgg gtaactgatt atttaaaaaa tcaaatgctc gattttgcaa aagactataa
    13321 gtttgaagag gcaaacagca ttaaacaaaa aattgcaatc atcgagtcct ataaagccaa
    13381 atctacggtt gtcagcaata aaatcaaacg catcgacgta tatggtttgg ctgaggataa
    13441 ggattctgtt tatattagtt ttttaaagat agtggatggg gctgttgtgc actcgcataa
    13501 tgccgaaatg cgggcaaaat ttggtgaaac aaagcaagag ttgcttgttt cgtatgcatt
    13561 tgaaatgtgg agcaactccg gcgaaaaggc tcctgaagcc gttgttcctt ttttgcctga
    13621 atttaaaatt gaagggctca cttggactgt tccccggcag ggcgataaat tcaaattgct
    13681 tgaactcgca ggcaggaatg ctaaaatata tatgctcgaa aggcacagaa taatagagaa
    13741 aaaggacccc gaaattgcta ttgaacgtaa actgaaagca ttacagttag atttacactt
    13801 acgcgaaata cccgattatt tcgagtgctt cgataattcg aactttcagg gttcaaaccc
    13861 ggtagcatcg tgtgttgtat tccggaatgc aaagccatcg aaaaaggaat accgccattt
    13921 taatataaaa acggttgaag gtcccgacga ttttgcatcg atgcaggaaa ttgtatttcg
    13981 taggtataag aggataatgg atcagaatga agaacttcca aaacttatta ttgtggatgg
    14041 tgggaaaggg caactgagtg ccgctgtaga gatactgaaa aaacttggtg tatacggcaa
    14101 acttgctgtt attggaattg caaaacgact tgaagaaatc tattttccgg aagactctgt
    14161 acctgcatac ctcgacaaaa attcagaatc gctgcgactt atacgttttg caagagacga
    14221 agcccatagg tttgccgtaa gttttcatcg caaaaagcgg tcatcagagt ttttaatatc
    14281 agaatttgag cagatacaag gcattggaga gaaatcgata ctcttgctaa ttgataaatt
    14341 caaatccttg agcgaagtaa aaaaagccaa tcaagaagac attatttcct tggttggtaa
    14401 gagtaaagca gaaaaaattg catcgtactt tgcaatgaag aaataaaaaa gaggcgtttc
    14461 cgcctcttaa ttttatttgg tttcagcgtc tttttgtttt ttcttcataa attggtatgc
    14521 aattggtgtt gcaacaaaaa tcgaagaata ggttcctaca aaaataccca gcagtattgc
    14581 aaatacaaaa cctctgatta cttcaccacc gaaaataaag atagccaaca ataccagtaa
    14641 agttgtgaga gaagttccca tggtacggct taaggtgctg ttgatggcct gattcatggt
    14701 ttctttcagg ttccgttttg ggaactcccg gatgtattcc ctgattctgt cgaaaactac
    14761 caccgtatcg ttgatggaat acccaattac agtgaggata gctgcaataa atgcttgatc
    14821 tacctccatc gagaacgggg caatggaata aaacaacgag aacgctccaa tgataatcaa
    14881 tgaatcgtgt accaatgatg caactgcgcc cagcgaatat tgccactttt taaacctgaa
    14941 cagaatgtaa atgaaaatac ccaaaagcga aattaaaact gcaatgaacg atttcgacaa
    15001 aacatcctga gccacagcag gtcctacttt ttgtgagctt tgtcggtagg aggcaaggaa
    15061 ttcttgttga ctaatatttg ttggtagcat cggcttcaac ccaacataca gtaaggagtc
    15121 aatttttgca tcggtagttt tatcgctcga agaaattaaa tattttgttg taattttaac
    15181 ctgactgcct gcaccaaatg ttttaacttc gggtgcttcg gtaaatgtca gctttaggtt
    15241 tttagccact tcctgaggat ttacaacatt gtcgaatttt acaatatagg ttctgccacc
    15301 ggtaaaatca atgcccgggt taagtccgcg ggttacaagc gaaataatac tgatggcaat
    15361 aagaataccg gaaataacat aagcaatctt tctcttttcg ataaacttaa ttttaacatt
    15421 ctgaaaagca tttttactaa taggtgtctc gaaaataatt ttcttgtttc gtttgatttt
    15481 tgtgtcaaaa acaagacgag ttataaagat agcagagaac aacgaagaca gaataccaat
    15541 aattagtgtg gttgcaaacc ccttaatagg gccaactcca aaattaaaga gaataattgc
    15601 gacaataatg gttgttaagt tcccgtcgat aattgcagaa taggcattct tataaccgtc
    15661 tttcacagca agtgaaagag ctttccctgc acgcatctct tcgcggatac gctcaaaaat
    15721 aaggatattt gcatccactg ccataccaat ggtgagcaca atacctgcaa tacccggcaa
    15781 agtgagcact gcaccgaatg aagcaagaac cccaaatatg aagaaaaggt tggcaagaag
    15841 agccaggttg gctgttaatc cgggtcttcc gtaataaacc accatataga gcattacaat
    15901 tatgaaagct attatgaaag aattcagacc ggagtcgatg gctttttgtc ccagcgaagg
    15961 tccaacaatt tcttccgaaa gaatatttgc cggtgcaggc atctttcctg atttgagaac
    16021 gttggcaagg tcctttgctt cgttgattgt gaaatttcct gtaatcgagc tgcgtccacc
    16081 ttttatttcg ttttgcactc ggggatagga gtacacatat ccatcgagaa caatggcaat
    16141 acatctttct ttattatcac gggtaattct tgcccattca ttcgaaccat cggcgttcat
    16201 cgtcatgcta acttcggcat aagcactcgt ttgcccgaag tcttcccgtg cattggtaat
    16261 tacatctcca ttaagaacag ctccattttt tacgcgtcga agtgcaatca gttcaaatac
    16321 atcgtctgaa ttttcgaggg gtttaaatga ccaggcaagc accaaatctt tattgaggaa
    16381 tgctttcact ttttcatctc tgaaaatagc gtttatttca gcagtatcgg ttgctactgc
    16441 tcttcctaca accggccctt ccattaattg tccgtctttc gatgccgatg ggtttaaaat
    16501 aacaaaaagt gggtgctgcg atttagcaag agctttgtcg ctttcctttt tgtcattggc
    16561 agcggtagcc ggtttcactg cagcattagt gtcgggggtt tcggtaacat catccccagc
    16621 aataacattc tggtttggaa caggtgcagt gttttggact gtatcggttg ctgcctcaac
    16681 attggttgta tcgggctgac ctcgtttggc aagatattca tcaatttttt cgagcgattt
    16741 gtacacttct ttattctcga atgtttccca gaactccagt aaggcagttc cctgcagaag
    16801 cttgcgaacg cgttcaggat ctttaatacc gggtagttcg atgtgaaaac ggcctttaac
    16861 tgcaaaatct tcctgaatgt ttggctgtac tacaccgaaa tggtcgatac gtttacgcag
    16921 aacttcgttg gcattgccta tagctgcagt gtattcctct ttaagataag taattacctg
    16981 agcatcggtt gatgatatcg aaatcttagc gtttcccgaa gtaatgaaaa tattggcaag
    17041 tgattttccc ttcgaaactt ccttgtatgc ttttgcaaaa atatcaacga agctttcgtt
    17101 gctcgacacc tgtttgtcaa cagcaatttg aagtgccttg ttcaacacgg tatcgctacg
    17161 gttattcgat agtgcacgta caatatcggc tgccgatact tcgatagtta cgttcatacc
    17221 gcctttaagg tcaagaccca ggtttaactg cctttcctta atttcgttgt aagtatattt
    17281 cttaaacatc cacagcacac tttcgccact ctttgtgagc gaatcgaggt aatgcttctt
    17341 aatactatcg agcttgtgcg atttttccaa atcatcaagt ttcagcttca tgcccgaata
    17401 tttagcttcg gcatatttgc ttgcccgatt ctctatctgg taggttctta cggtaaaaga
    17461 aagatagtaa acacaaatca gagtaagtaa aactgctaca agaataatag ctcctttatt
    17521 acgcatttca gtaaaattta acgtttatac tttttaaaat tagcttgcaa atatagatta
    17581 ttttttagaa aatacgataa cttattcaat atttagatgc aagcgaagtt ccttgtccaa
    17641 ataacttatt tcggcccggt attttagcag tatatcaccg ccgattatac ccgtgatttt
    17701 atgctttccg atggtatgat acatggaatt gatgtggtcg aatggaataa aaacagagaa
    17761 gtaattccgt aacacaaagc caccgagagc aaaactcttt attgttgaca catacatttc
    17821 gctgatactc gagttaatac ccgacgacgc attctcttct gtaattttat tgaattcgtt
    17881 atcagaaaaa acttcgctgt ttttatcaaa aaccgattgc gatgctccag tatcgataag
    17941 tacaattctt ttttttcttc caatgcggca ttgggcaaac agatgtatcc cttgtttgtc
    18001 gagctcacag attatcagtg gtacgctgaa aacctttatt tggttttttt taagttgttt
    18061 caattccggt aatatttaat ggttttctgt ccttaatagt tttaattttt caaacttaac
    18121 taaaaatgct ttaaacgaat tggatttcag attgtcaaaa caacataatt ttaaaactta
    18181 cttatatgag aaacaagatt attttattga gcattgccgt aatgatgatt accggtgtgt
    18241 caatggcgca gaaagccgac tgcaataatt cgaagaagga acaagctaca ctgtacggta
    18301 ttcaggacct tacacccgaa caggaaaagg ctattcaaac aattaaaaag gatatgcatt
    18361 ccgtacaaaa aaatctgaaa gctgaacgtg atattaagga agcagagctc aataagctgt
    18421 taatacagga taaggccgac cttaaggcaa ttgatgctaa aattgatgag attacagcta
    18481 ttcgtgcgca aatggaaaaa aacagagttc acgctaatat gaatatcaga caattgctga
    18541 atgaggatca gaagatgcag ttcgaccata aaactatggg acagaataaa gcaagatgcg
    18601 ccgaagggga aggacagatg cctgaaaata aaccatgtgg acacaatgac catcaaaaca
    18661 gcggggaaaa ggctccggga aaccataaag agtgccccca ttctcaggat aaaaaataat
    18721 agttgaagaa ccttgtatta ctgcttttgt gattttttcg caaaggcagt tttttgtttt
    18781 attcaaattt aacagtagat ttggattgat tttttacaga atttcatcaa aaaagaggcg
    18841 gaatgaaaac tatatgggtg ttttatgtac taagtctaat tgttgttcct tcttacagtc
    18901 agtgggtttt tcagaaagaa attccttttt ctttgggcaa cgttggcgtt gatatattag
    18961 gcaatttgta tgtttttgat tcttatcaga taaaaaaaac cgatagtaat ggtaaggtaa
    19021 ttttcagcta cgccaataaa aacctgggtg aaattaccgg tgccgatgtt accgacccaa
    19081 tgcgtattat gttgttttac ggggatttta attatttggt tttcctcgat aattccttgt
    19141 cgcttttacg tgatcccgtt tcgctcgacg gattgaatct gaacagagcc gaggtggttt
    19201 gtgcttcgca gaaaggtggt ttttgggttt tcgactctgg cgatctccgg cttaaatatt
    19261 ttgacagcaa tctgaaacag ttgaacacaa gcagtgctat tgatgtagat gtttcaaacg
    19321 ataaatccat ttccctgaaa gaattcaccg attatgtatg ccttgttttc gaaaaaaacg
    19381 ggtgttttgt tttcgataaa ttcggaaact tcgtatttcg gatacaggaa caaggctttt
    19441 ctgctcctta tgtaaacaat gggtatttgt attatatctt gaaaaatgaa ttggtcgagc
    19501 ataacctgaa gttgcacaat gagacaaaaa ttgcgcttcc ggttgataac agtattcagg
    19561 ttttattcaa tggatcgttg ctttatgttc ggcaaggcga caaactgcaa atttattttt
    19621 tagttgaaaa ctaattcatt gttaataaaa caaaaaatct tattttagtg aattaagaaa
    19681 aacaacagtt tatgcacatt gcaatagcag gaaacattgg ctcgggaaag acaaccttaa
    19741 cacggttgct tgccaaacat tattcatgga cgcctcattt cgaggatgtt gaggacaatc
    19801 cgtacctaac cgacttttat gacgatatgc aacgatggtc gtttaatttg cagatttatt
    19861 ttttaaatag caggtttaac cagattctcg acattagaaa gtccggtaaa acagtaattc
    19921 aggatcgcac catatatgag gatgcctata tttttgcacc caacctgcat gcaatggggc
    19981 ttatgacctc aagagatttc gataattatt tttcgctgtt caatctgatg agctctttgg
    20041 taatgccgcc cgatttactg atttacctca gggcatcggt tccgacttta gtgcagcaaa
    20101 ttcagcagcg agggcgaaaa tacgaaaata ccatccgact cgattaccta cagcgcctga
    20161 acgaacgata tgaagcctgg attaatggat ataagctagg gaagttgctt attgtggatg
    20221 ttgatcatac cgatttcacg gctgtgcagg aagacttgcg cgatgttatc aataaaatcg
    20281 acgcggaaat acacggtctt ttttaggatt tcgtttatag gcttttaaaa gcagcagagc
    20341 actacggatt aatcattacc tgatgcagca atgtatcatc cccgtggcgt aaagttattc
    20401 atgaaaatcc gcgttcctaa tagtattttc aacctcaaag gccacctaag atgacaatcc
    20461 actgaaggca tcgcaacgcg ggcggccccg acaaaaatgc gagccagcca aggcgtgaat
    20521 agccgtttaa tagcaaaaaa caacaaaggg caagagaagc aaacaatcta tagacccgaa
    20581 caaggtttgc ttcacgcagc tttgttttgt cgggcaaagg agaaaggcca gtgctgacaa
    20641 aacactactt tgttagtttt ttcattaaac gggtagagct gggaggagca tatttggctg
    20701 gtgagcgcag ccgaaccatg tcttgacttt tgggttactt tttgtgtcaa gacaaaaagt
    20761 aagagaaaga attatttatt gattttaaca tctgtatctt taacagtggt aactagtttt
    20821 ttgctcaaaa aagctgcaca aaatggaaaa gtcaagaata aaatcgacgc ggaaagacac
    20881 ggtctttttt aggatttcgt ttataggctt ttaaaagcag tagagcacta gggattaatc
    20941 attacctgat gcagcaatgc attccttttc agcgtttatc cactcccatg tttcagaact
    21001 catagtagtt ccgataactt ctattaccct tgacgccatt actgttccca actgtccgca
    21061 ttttttcagg ctccagccgt tggcatgacc atataaaaat ccggaagcat agttatcgcc
    21121 ggcacctgta gtatctacgg gtgatgtttt gcgggcagga attttcgata attgtccttg
    21181 acttttaata agcgacccat cagcacccgt tttaacaatg gcaatacgac acatttcggc
    21241 aatttgatgc acagcatctt caggtcccat tccggtaagt gcacgggctt cttcttcatt
    21301 ggcaaataca atatcaacat cgttttttat tacatcaagt aaaaaatcac gattgatttc
    21361 aaccacatta tagcttgcca tatccatcga aacaataata ttgttagccc gtgcaatttc
    21421 cattgccttt tttatcaggc tgtgattaaa tgcaaggtag ccttcaatat gaagataatc
    21481 gaaacctgcc atactttctt tgctcagctc ctcaggggtt aattcggctg cagcacccag
    21541 ataggttgca aacgttcgct cggaatcggg acttaccaaa gcaacagcaa gccctgttgg
    21601 cgttgcactg tgctgcaata gtgtgttgat gcccaatgca ttcatttcat ttctgaaaaa
    21661 tatacccaat tcatcgttgc tcaccttccc aagatagcca cagggtgctt tcaggttggc
    21721 aatgcttcga atggtgtttg ctgccgatcc accgctaact cttgtgcgaa ccatggattt
    21781 agtaaattcc tgaatctgtt ttgattgcgt tgcatcaaca agctgcatgc tacccttcgg
    21841 aagtgccaaa tccgacaata tgctgtcgtt aggaagctgt gtcatcatgt cgaccagcgc
    21901 atttcccatg gcaagtattt ttttcatgat aaaattttaa aattattttg caaatataga
    21961 aaaacctctt acttttgcca cacttaaaat attcctcctt agctcagttg gttagagcac
    22021 ctgactgtta atcagggggt cctaagttca agtcttagag ggggagcata aacagagtga
    22081 gagtgagagc gagtacagag tacgagagat cactctcatt ctgtttatat aaacaaccct
    22141 atttaaaagt taaccttaaa ttgcaatgaa aatgcaagcc gccatgcgtt ttggcagcat
    22201 cctgtcgcca tacggttatg aagcttaatt caggttaacc ttattcttgg taaccacaat
    22261 cccttcaaca aaggtaattt cgatgtagga gccatcctta tttgtccatg tatagagttt
    22321 tgcactgatc ttccctgcgc cttgctggtt tgcggtgctt gaggcctgtg tcaggagtac
    22381 accgtccttc agaagtatat tttttacgtc agccaaattc atcccaactt ctattttatt
    22441 gtacatatca actgtgtact cgtttacggg tttaacctca cttgtttttg ttgtatcggt
    22501 gttgttgctt tgtggtttac ggggagaacc ggaacatgcg aacagggcaa aaacgagaat
    22561 taaaagggct gcatttttca tgatgtttag gtttttcaat tagaatgttt acttcgattg
    22621 gtattacttg ttcaaatagt tatgacagaa tgaaatttga catttaacat aaggtaaaaa
    22681 taacaaatta atacaaaata aatcattttg aaaataattc catcagcaga atgtgttttt
    22741 caattcctct cccaaaagca ccatatcctg tactattgtg atgcccgtct ttgctccaga
    22801 aataattcaa aacagttgcg ctatccattt tttctaccac agtatagtat tccattaggt
    22861 cgcaatagtc gatatgcgct tgtttggttg cattaataac tttctgtaac gaagagctgt
    22921 tgttcaccac ttcccatgat aatggctgaa aagctaccaa caatcgaaac ccgttttcat
    22981 ctgctaattt cttaaaatcg tataagcact gaataatctg tttttcagtc ctagtgctta
    23041 aatctcctga attacggtcc cctgttaaaa actcatcgta tcctaatgct tcaaaaacaa
    23101 ttcgggaaag atgagatgaa gcgtaaatcg gctcaaacca aggtgcatga ttatatttta
    23161 cacttccatc aggttgaaac cgggccatgt caccacgtag cattacatcg agtatatctg
    23221 tgctgttgat acatacaatc acaaggtcgg gcttgtattg taaaagcttt tccctgagca
    23281 gtatatattc aaaatacggg tcgctgccac aaacacctgc attcataaat cgaagatgca
    23341 atgggtaatt gtatttttgc agacctcttt ccagaaattt aagccatgta gaatccgcat
    23401 cggcaccgtc tccttctgtg aaagaatctc ccaacgcgat aatacgatat tcactactat
    23461 ctttttcaac tggatgctca atatctgata gtccaagtga attgatgctt cggtaatagc
    23521 gatattcact cgattcgaga tagtgattcc ggtgccatac atgaaaccag tttttatctt
    23581 ctgcagcata gtgcgatgcg taatatagct tatatctttt ttcaagatag gtgctcttgt
    23641 atcctgtgaa aatgaaagat aattctaaaa ccactaaaac aagggcaact gatatggtga
    23701 ccaaaaaaag gttttgttta ttgatatttt tacgtaataa tctgctggtc agaaatataa
    23761 gataataaag gaaaatgctg ataccaataa ttaatataac accagaactg atgaaatagc
    23821 attttgtgaa aacgaaaaat agaatatgaa cagcaactaa taacaaaaag tataaccata
    23881 aaaatacttt tctataaagt agattttgag ggcttcctct tttcgcaata tttttgatta
    23941 cactgattaa gttcataaaa ctgaaagttt ctagctgacc ttttttatta aataattatt
    24001 gcgcttcaaa tagttaaata cttcatttgc aaaaaactta ttcaaacgca accccgggtg
    24061 tccgtttaca ggagacgcga acaatacttc aggtggcagt cctttgaact tatttgcaaa
    24121 tgctggatac agattaagat aattcaggtt attccgcgtc attatatcta ctatcttatt
    24181 attccatctc atgcgagtgc tgtcagcaga taggggagtc aatacaaata atatttccgt
    24241 accattgctg ttaacactat caacaaaatc aaggattagg ttttcgtagc ctttcaaatt
    24301 aaaatcggag tagaggcctt cctgccattt cttaaattca gccggttggt cgtcgtaact
    24361 taatgaaggg tcgttttcaa ctaccccgac aataagcaaa tcggaatgat agcgaaatcc
    24421 gttattaatg aaatatctgt attcgtgaag ggtactccaa ccacataaac cccatgatat
    24481 tagttcggtg tttttgtatt tttccagtat tttatcttca agcaaatggc tccatgtata
    24541 tttataataa attccatcgc cccatatcca agaatctcca attactgcaa ttctgaaaat
    24601 attatcaggt tttattgtat ctctttgaat atcggtgaag taatcctggt tgttctcagc
    24661 aaataaacgg ttgatttcat taattgagcc tttttgtttt tgccataagg ccttttgttc
    24721 agggctgtat tcagtaatca cactgaccgt tttgggagta cggcaacata tcactgtaag
    24781 aagaatacct gtaaatacaa ttacaatagg gctaagaata attattttta atgtaaaaaa
    24841 gagcctgccc ataatccgtc cttagttttt gtccaaaggt aataaaacaa tatttgtccg
    24901 cccataatca ggcaatatta aagaggtaat aaattcttga agttgaattt ttaatgtttg
    24961 aaatattttt aaatcgtttt aaaataaact aaatttactt ttgtgaaaat actttaacaa
    25021 tatggaagcg acgtattcta gaatcaaagc attatcagag aaatacagcg attatactgc
    25081 aaaaaactta tcggaattgg ttaaaattaa atcactcagt ttgcaggaaa aagatgttca
    25141 gctggcgctg aaacggcaga tggaggaagc gggtttcgat gaggttttca tcgacggatt
    25201 gggaaatgta atcgggcgta ttggtaacgg aaaaaaagtg ttggcaattg atggtcatat
    25261 ggatacggtg gatataggaa atactgccaa ttggagcttt gatcccctcg gcggagagat
    25321 taaaaacgga tttgttcatg gaagaggcac tgtcgatcag gaaggtggac ctgcagcatt
    25381 tgttactgcg ggcagagttt taaaagaact gggttttaac cgtgatgtga cagtgtattt
    25441 cgtgggcagt gttatggagg aagattgtga tgggctttgc tggaagtata ttatagaaga
    25501 ggataaaata aaaccagatt ttgtgataag cactgaacct acaaacctga atatatacag
    25561 agggcatcgg ggaagaatgg agatgcatgt acacttttat ggtgtttcgt cgcatggttc
    25621 ggcacctgaa cgtgggaaaa atgcaattta tatggcctcg cgtgttgccc ttgaaattga
    25681 gaaattaaat gagaagcttg catacgatga attcttaggc cggggaagcg ttactatttc
    25741 tgaaataatt tccgggagcc cttcgctatg cgctgtagcc gattatgcac gggttcatct
    25801 cgacagaaga ttgacctggg gcgaaaccaa agagtcggca gttaaggaaa ttgaagctct
    25861 cgttcaggga atgaatgcca aggttgaagt gctgaactat tatgaaactg catatacagg
    25921 cctgaaatat ggtatggaaa aatattatcc gacatggaaa atgcctgaag atcacgaaat
    25981 agtggttacc ggtgtgaaag cgtatgaaaa actttttgaa aaaaaaccga ccattgataa
    26041 atggaccttt tccacgaatg gcattatgac caatggcgtg tatggcattc caactattgg
    26101 ctttggaccc gggaacgaag ttttggctca cgccccagat gaaaaagtcg cggtgtcgga
    26161 tctggtagtt gcggctgctt tttatgctgc ttttgcattc gaaatgtaac aatcaaaaac
    26221 tgaacaacat gaaactaatt gagaaaattg aagccttacg attgctaaaa tccggactgt
    26281 atcaaaagga tttcttgttg acctgggaga aaacggaaga ggaaatcaga ctaatcctcg
    26341 aagtggcatc catcatgaaa gaaatgcgat ctcagaatat ttcgctgcgg gtttttgatt
    26401 ccggactggc aatatcgaat ttcagagata actccacaag gacaaggttt tccttcgcct
    26461 ccgcaagtaa tatgcttgga ctggctgttc aggacctgga tgaggaaaaa tcgcagattg
    26521 cacatggtga aacagtgcgt gagacagcga acatgatatc gtttatgtct gatttcatag
    26581 gaatacgcga tgatattttc ctgggtgaag ggaacagata tatgagggaa gtcggtgctg
    26641 ctctggacga agggttctcg cagggggtgc tgccagtgcg gccgggtatt gttaaccttc
    26701 aatgcgatat cgaccatcca acccaagcaa tggcagacct gatgcatttg aagcattatt
    26761 ttggctccct cgataaactc aaaggtaaaa aaattgtgat gagttgggcg tatagtccca
    26821 gttatggcaa acctttatcg gttccccaag gtattatcgg actgatgtcg aggtttggaa
    26881 tggacattgt acttgctcat cccgaaggat acggattaat acccaatgtg gtagagcgtg
    26941 caaagactca ggctcagcaa agcggaggca gtttcagcgt aatgcattct atggacgaag
    27001 ccatgaaagg agcagatatc gtttacccca aaagctgggc tccataccat attatgcagc
    27061 aaagaacaga gctgttgaaa aatgctgaca aaaacgggtt gaaggagctt gagcaggtgt
    27121 gccttgccaa caatgccgta tataaaaatt gggaatacga tgcaactaaa atgaaatcca
    27181 ccaaaaacga agatgcattg tacatgcact gcctccctgc cgatataagc ggagtttcgt
    27241 gtaaagaagg tgaagttaac ggagatgttt tcgaaaagta ccgcataaga acttacattg
    27301 aagctggatt caaaccgtat ataattgctg ccatgatgtt tacaaatcgt tttgaaaatc
    27361 ctgccgaagc attgcgcaaa ttggttgaac gaaaccgctt gcgtgttggt gctttgtagc
    27421 ggttaataaa atcacagttc gggtctgttt cgctttaaaa atctaccgcg gcttatgcta
    27481 gaaaacagta tgtcgttgtt aacggcaatt tcacctcctt tcactatata ctctgcatgc
    27541 cccgaaaccg aaagtccgtt atagatgttt atgtcgcaat gctgatgctg atttaataga
    27601 gaaattgaat ggctgccatc aggattccag atcacgatat cagcatcgga accgattgca
    27661 atgcaacctt tctgcggaaa caacccgaat atctttgccg gcattgtgct gaaaatatcc
    27721 accattttat tgatgcttat cttcttcccc agcaccccgt atgtatatag taacggcaaa
    27781 cggtattcga tgctgccggc cccgttgggt atgctcctga aatcgtttgc cccggctctt
    27841 ttttgttcca tattgaatgg gcaatgatcg gttccgatag tgcttacaac atttttgtta
    27901 atggcatgcc atagtgcctc attatcacat ggctcacgga gcggaggact aataatataa
    27961 ggtgctgctg acgcgaaact gcctgtaatc ggcgggttat tcagcagtaa ataatgtgga
    28021 caggtttcag cataaacctg ttgtcctttt tttttggctt cttcaatgtg aaaaagcgat
    28081 ttttttgtag atacatgcac tacgtaaaaa ctacagcccg cctcctgagc aagagcaatc
    28141 gcctttttta ctgaatccga ctccatttcg gcaggcctgg ataggtaatg atagagcggt
    28201 tctgtcttgt ttacagagaa ataataatca cgaagccttt cgatttcttc accaagttca
    28261 cagtgcgcgg ttactatgcc ccccaattct ccaactgtgc gaagcacttt cagaaaatca
    28321 gcatcacaaa gaccgacggt ttttcggtat gcaagatata ctttgaaaga agtaatccca
    28381 ttgctgaagc aatcggaaat ctcatttctg gtcccgctgt gccattctat cggacttaca
    28441 tggaacgaat aatcaatcag cgaattttct gcctcgcgtt ttcgcttttc cagtgcattc
    28501 ggcagacttt ctccttttac aggagtgaca aaatcgatta gggttgttgt tccaccgtac
    28561 aatgctgcaa tactgccggt acgaaaatcg tcggatgaag gccctgcagg agtaggcaaa
    28621 cacatatgca catgtggatc aacacctccg ggaaatacga attttcctga agcatcaact
    28681 attcgtgtgg ttgctgaatg cttcaggttt ttgccgatag aatgtatttt accctcatgt
    28741 atataaacat cattcacaac cgaatattcc gaagttataa tgttgccatt cttaatgagt
    28801 atatccatat gaattgctaa taatacaaag ttactgttta ttgctctatt taacacgaaa
    28861 tactttatca gtggataata aagttgttaa tttgtctctt tgcttggcta tgactacaat
    28921 ccgctgatgt atgcatcaag gtaattgagc cgtgcaggta tccatgtctg gataaaattg
    28981 cgttcttctt caatgtttag gtttaagctc cagatactgt tctcgagtga tatgatgtcg
    29041 gattttgaga ttttttcaaa atctctttca aacaacgaca aaatttcggt ttcagtgaaa
    29101 ttagagtttc gaaggctaac ccatcttgtt ttaagtcttt ctctaaaatt gtcagggttt
    29161 aagttgataa gcctgttgta aagattgttg gtgagccggt ttgtgtaacc aatatacgtg
    29221 ccgtcccaga aaagccccca gcttgcatct atatcccaag gtataataaa gaatttatca
    29281 gtattccctg tttgtgccag aaatgtattc ttgccaacat tgtcgagggc tgttaccaga
    29341 ttgagaaata aaaaatagtc gataaaatta tccatatcaa tcaggtcggc tatactatcg
    29401 cagaattcag catcggaaga gtttacaaca aaatcgcgca atttataaag cggcatccag
    29461 tttgttttta attcgggatc cggaaatttt tgttcccatc cgtcccagta ttgtattgtt
    29521 gagggcattt cgggaggaat atcgaaagtt actgctccgt ttgcccaatc gtaagcctta
    29581 taaagtactg catctggctg cgaaaattgc agaagttctg cagtgatatt ttcgttgaga
    29641 caatatagcc cctgatggct attgttaatt accagctcaa ccagttccga ttgcagacca
    29701 tcgtgctggg tattctcacc catgttacgc cagatttcga aagaaacctt atttcggagc
    29761 ctacccttgt cgatgtacat ggcatcaaga atccagtttt cgagtaacct cattgaaaaa
    29821 agctgacatg atctgcgatc tttgatgttc atgctaccca gtattgcaaa accatacgat
    29881 tttttgggaa ataattgtgc tgttacacca cggtattcta tcccgatata gctgctgtaa
    29941 cttggtcttt ccggctcagg gtagtgaatc gtcaaccgag cgagcgtctt tggttcgtcg
    30001 aaaaacccac tgatagttgt gatttgaaca attggtaaat ttgtaaaaac cagggtaatt
    30061 tccttggtaa aatcaccggt ctttacaata atagggtact cctggtttat gtgaaccgtc
    30121 ccaatgttat ttatggtatt tcggctcaat tgcctgttag caaaataaat ttcggaataa
    30181 tcctgaaatt cggtaaacgg ctcaaagtga acaattgaat ctgcattgac aggataacgc
    30241 aacaaatttc tgcctgaatc gaacacgcaa tcttttccgt taaaacgcaa aatggtggac
    30301 agttcaagag agttgtccgg ttgcaagtcg tatatcactt cttccttgta acaggaactc
    30361 agcaatgctg ctgtcagaat acagataaag ctgataatga cggacctcat tatgctatag
    30421 ttttagagtg tacgaaattg ccataaaaac aaatgatgtt ctgtagctta ttttgttctg
    30481 taattcatat cctatggcaa ggttgagtac gcctgattga atacggattt cgtcacctgc
    30541 aaaacacctg attttgctga aatagggatc cctgagtttg tcggtggtac ggaagatttc
    30601 acccgatgtg tacaaagaat gcatgccatt atattggtat tcaagcttca aacgataccg
    30661 aaaatcggtt tcggttaatt tttcctggct aattttgtta aacgcatcaa agtactcctt
    30721 cgaataccgt gcgcgacaaa ataacttaaa acgtaagcca aggcgtgctt tataattcat
    30781 atcgaagcca tatcgcaaat cattctcaat tacttcgtct ctcttaatat tatatgtata
    30841 acgtccgttt gccccaatgc tgaaattccg gttaattgag taatcgatac ccaaatccga
    30901 tatgcaatca tcaatttttg tacaattatg atacaggcga agctcctgat tgaactctgc
    30961 ctgaaagtcc tttgtgattc tttttcccac cccgatacct gcccataacc ctgcatctct
    31021 aaccacagca atttcctgcg atatcaacaa actcgaaatc aggagagcga aggaaaaaag
    31081 agcaatatgt ttcatacagg taaaagaagg cttgtattac ataacaccaa tgcaacaaag
    31141 atattataat taaatgtatg caataacctc cattaaaaaa aagaggttga tctgtgtgac
    31201 caacctctca aatttataaa gcataaatat cgaattagaa cgaaaaagcc agtgttactc
    31261 cagccattac aatagaaacc atactcatta atttgataag gatatttaag ctgggtcctg
    31321 atgtatcttt aaaaggatca ccaacggtgt cgcccgtaac tgctgccttg tggcaatcgg
    31381 aaccttttcc gccaaaattt ccttcttcga tatatttttt tgcattatcc catgctcctc
    31441 cggcgttggc cataaataca gccaaaataa aacctgatga aatgcttccc agcaataacc
    31501 ccattactcc tgctacaccg aaaactattc caacaagaat tgggatactg atagctatta
    31561 gtgcagggaa catcatttct cgctgtgcag cttttgttga tatagaaaca catctggcat
    31621 aatcgggagt tgctttgcct tcgagaattc ctgcgatttc gcggaattgc cgccttactt
    31681 cgtcaaccat tttttgagca gcacgtccaa cggcattcat agtaagtcca cagaaaagaa
    31741 atgcggcgac agcaccaata aaagcaccca caagtacttt ggggttcatc agatgaactt
    31801 cgaatgattg aataatatca atcattcctg cttcgctggc tttaacattc tcattaaggt
    31861 taggtaatac agggttatca tgaaaatgaa taagaccttg tcgcacctct tcaataaaag
    31921 aagccagaag agcgagggct gttaaagctg ccgagccgat agcaaaacct tttccggtag
    31981 ctgcggtagt atttccaagg gtatcaagtg catcggtacg tttacggact tcatgttcca
    32041 gtccgctcat ttcggcattg ccaccagcat tgtcggcaat tggaccatag gcatcggttg
    32101 ccagtgtgat tccaagtgtg gataacatcc ccacagcagc aattgcaata ccatataatc
    32161 ccataccaaa atttgtcatt tcccaatttg ctgaaaaccc aaatgcaagc ataatggctg
    32221 ctcctacaat gagcacggga aatattgttg atatcatgcc ggttccgatt cctgcaatta
    32281 ttacggttgc atgaccggtt tttgatgcag cagagatgcg gcgtgtaggt ttataagatg
    32341 atgaggtaaa atactcggta gcctgaccaa ttcctatccc ggctataagt ccgacaatca
    32401 tcgaccacca gatacctgca tagtttggta attcaagtaa gcgaaggata aaataagagg
    32461 ctatggctat cattattgca gatgcattga cgccattacc cagtgctcgt aataattttt
    32521 tctgagaggc gccttctttg gttcttacga tgaaaattcc aatgatggat aaaacaattc
    32581 cgacagcagc aattagcata ggagcaatta ctgccttgta ggctgtttct gcaccgagat
    32641 agatgaatcc tgctgcaccc aaagcagcag tggatagtat tgcaccgcaa tacgattcgt
    32701 aaaggtcagc acccatacct gcaacatcgc ccacattatc acccacatta tcggcaatag
    32761 tagcaggatt gcgagggtcg tcttcaggaa taccggcttc aactttacca acaaggtcag
    32821 ctccaacatc agctgctttt gtgaagatgc caccaccgac tcttgcaaac agtgcttgtg
    32881 ttgaagctcc cataccaaat gtaagggttg tggttgtgat aattagaaga ttattcggat
    32941 cggctgatgg tggataaacc aattgcagaa tgtaaaacca aatcgagata tcgagcaatc
    33001 caagcccaac aactaccaga cccattacag caccacttct gaatgccagc ttcaagccgg
    33061 agtccaaaga ttttcttgct gcattggcgg cgcgtgccga cgcataagtg gcagttttca
    33121 tgccaaaaaa acctgccaaa cctgagaaaa accctcctgt tagaaaagca aagggaacaa
    33181 acggattttg aatttcaagc ccatatgcta aaaatgcaaa cagtacggaa attactgcga
    33241 aaacaatgag cacaacttta tattgctgtt ttaaatatgc catagctcct tttcttacgt
    33301 gggaagcaat ggtcttcatt aattcggtgc cttcatctgc acgcatcatt tgccggtaga
    33361 aaaaataagc aaaacccagc gccaataccg aagcaatagg tacaatccaa aataaatatg
    33421 attccatagt ttattattta aattaagggg caaatgtaaa aattaggtgt gatactcaca
    33481 aatataaaat gcaagtaata gtcaaaaaga tttacccgat tattcaggta agttgtaatt
    33541 aatcaactta ttgcattctt ataaaagcgg taagcctctt ttccggaatt cgtaacaaat
    33601 tattccggca gcaacagcaa cattaagcga ttccatctgc atgtttggtg agtggtaagc
    33661 cggaatattg atttttgtat caatcagaat ttctagccgt tgcgaaatcc catttccttc
    33721 gttacccagt ataattaaac actgttgaga aagctttgac acgcccagag ggttgcctga
    33781 aagaaatgtc ccatacacgg gattttttgt ttgatgctta tatcttttaa tgtacagact
    33841 taggtcggaa tagtatatgt taacccgcag gcaggcgccc atactcgact gaatcacctt
    33901 tggattgtag gcgtcaactg tgctctcgga acaaataatt gtagtaatgc caaaccattc
    33961 acataatcgt attattgtcc cgaaattgcc cggatcctga atgccatcaa gagcaagagt
    34021 gatttctgct tcatgtaaag gtgggtccga ttttgcaggt ttctcgaaaa cagccagcac
    34081 ctgtgcaggt gatctgaaac tgcttatttt tttcattgtt tcagatgata ccggtgttat
    34141 ttccaagttg gttactgcca agccagattc attggattta atccagtcat ccgttgcaat
    34201 gaggtatttg atatttacac cggattccaa agcatcttta atgatttttg taccttcagc
    34261 aacaaataat ccttcaatgt cgcgattctt tttatcggcc agcgatttta tgcgtgctat
    34321 ttgtgctttt gaaagcattt ggaattagtt tgtacaaaaa taatctttaa tccgagtgta
    34381 ggtttcgctg cttattactt tttgttttaa aagctcttcc gtagagttaa ttctaaccac
    34441 cattgtacgg tagttcagaa tggcaattac atcagttttc tctaggtatg ggtgtgaaat
    34501 cagtgtttta aagtcacaat cattgagatt gatacaatcg atttttgatg catccgcata
    34561 cacatgaggt tttaccgaag taaatagctc ctcagtcatg ccatatactt ctctgagttg
    34621 ttctacatgt gcgaaacctc ccagtttttc cctaaaactg ataatgcggc ttgcataggc
    34681 attcccgata cctttgattt taatcaggtc ttccgcagta gctgtgttaa gctcaatgtg
    34741 cagattctca atcggtttgt tttggttggc ttcaattttt atccagggtt ccagattatt
    34801 gtaacatgcc gaatcgaaac tatagattga aagaagatca gatggttctt taaaccggcc
    34861 tcctttgctt ctgtatttta caatatttgc agccatatat ttttttatac caagcgacat
    34921 tagctcttct tcgcttgccg tatttgggtc aaaataaaaa tagtttacct gcggagcttg
    34981 ttctctgctt gattgaacac tgttttcaaa taccagataa ggctcaagtt tttcaaattg
    35041 ttcgggtccc agcccgtaca ttctggcaaa atcggcaggt tttcgaaatt tcccgccttt
    35101 gttacggtag ttgataatat tgtgagctgt gtttttatta attcccagtt taattaaccc
    35161 gttttcatca atagtattgg ggttgaaata gaataattct aaagccgaat cattcggtga
    35221 gttttgattg cgtcgcgtaa acgcatcgat ttccgattca aaagccgaaa aatcatatct
    35281 tttattaaat gatatgcgat gcataattgc ctgcgatgaa atcagcaata ttataattgc
    35341 acagagtact atgagcccac gcttttcgcg ccgcgaaaaa ttaaagtaat cattaaaacg
    35401 actcatttta tcaataactt ttattcacca gttttttaca tgtgtctaat tttgttggca
    35461 atcttttccg gacgaaatgc aattgattac aaaactaaaa ttaatactaa aatctttaaa
    35521 ttgaaaaata tcacataaaa ttgccattat atttcatgtt aaaatgaaca atttctagaa
    35581 ccgttgttaa atcaacggtg tgttgttaag taattaaatg attgatttat ttatttgtaa
    35641 aaaattggaa tattggtaaa taaaatgtat ttttgtgtta aatatagtta ataaagataa
    35701 ttaaattttg gttatggata aaattagatt ggatgcgatg gataaaagaa tcctcgaact
    35761 cctgataaaa aactccaaaa cacctgtggt tgatattgct aaaaaatgca aaatttcggc
    35821 tgcagcaatt cataaacgta ttaataagct ggaagaagtt ggggtaatcg ggggctacag
    35881 gcttgttgta aaccctcaga tacttgattt taaaacacag gcttttatag gaattattct
    35941 cgaaagagcc aatatgtttg caggggttac cgaaaaaatg agagagatcc ctgaagttgt
    36001 tgagcttcat ttcaccacgg gacagtacgg tattttagcc aaggcatatt gccgcgacaa
    36061 taagcacctc caggatgtac tcgtgaagat tcacaatatt acaggagttt cgaataccga
    36121 tacaatgata agcattgagc agttgctcga tcggagtata cccttgtagc agttaaattt
    36181 tttgcgataa tttttctatc tggtcactaa gtttcgaaat tttttggttc agttcatcga
    36241 ccttagcttc cagttcttta gtattgtcag caaccatagc gtcaacaaat atcgaattca
    36301 ggagcgaaat accaagtact ccgcccgata ttaatattcc aacaaaaaat attgttgata
    36361 tcactccgat tgctgggctg gtattggcag caatagcatt tggaatttcg taccatcctt
    36421 caatggtaaa aattctgaaa attgaataaa ttgatgtaat agggtcacca aaacactccg
    36481 gtagaatgtt cttgtaaata tggcatgaga atattccaag tatgaaatta tacacaaaaa
    36541 gcgaaatcaa caccagcagc gatgcgcgga acgctcttct tataccaccc aacaaaccat
    36601 tgatattggg aatgaatctg atgaaaagaa aggttttaaa tacccgcgca acccgtataa
    36661 caataacata acttaaatcg gctgcttcta tgtcaataaa ataaaagaaa acagaaatga
    36721 acgagatgct aattattaga aaatcgaaaa cattccaggt ggacttaaaa aatttattaa
    36781 taccgaattc ccttattttg acaagtattt cggcaacaaa tatcagcgtt agcaaaatat
    36841 ctgtagctgt tagaaatata gccgcttttt cgtgaattgc atcgtacgat aaagcgaaaa
    36901 ttaccacagc attcaaggca atcagcccga aaatgaactt attgtttagc agaagctttc
    36961 tcatgttaac ccaatgttta cctgcacttt ttgcagaact gaaaaacaaa caataaaaat
    37021 agtatttttg atttcataaa tacaaacatg agtgcaatta tcaagaaccg gatagaatat
    37081 attgatgtta tcaggggaat tgcaattttt ttgatgattg cagcgaattc tgctccgtat
    37141 attatgaacg aaccctataa ttttgccttt agggttttca gctctttggc agctccattg
    37201 ttcatagcct tgtcgggata tatgcttggt ctgcatagca cgaaccttaa gacctggaga
    37261 actgtttaca aaggcggatt aatagtactt accggtgtgc tggttgatgt ctttatctgg
    37321 gatctgatgc cctttgtttc gtacgatgtt ttgtatctta tcggtatttg tgttatgctg
    37381 tatccattga tatcattgat gaatagagtg caaaacattg catggattgt agctgtattt
    37441 gttgtttcat ttatttcgca atttgttttt aattaccaga ttcgtaatac cgcatttcaa
    37501 ttcgattgga ataaccttgg catagtattt caccagtcga agtttctgtt attcgacgga
    37561 tggtttccaa tttttccgtg ggcaggtatc ttcttattgg gtcattttgc cgggaaatac
    37621 acaattctat tgaagcgtaa acaaaaatta tatgttttga ttatttcaat tgcggtgtta
    37681 ttatttgccg tgtttttatt atttcttcag aatcgcccca cccgtgaagg ctattgcgaa
    37741 ttgttttatc ctgccgatgt tttttattgc ctgactgcag tttcacttct ggttattttc
    37801 gcaataattt ataaaatatt caatacaaga atatgggagc cactgaggtg gcttggaaga
    37861 agcagcttgt ttttttacgt gcttcacctg ataatcatta ggttctttat cgaaaagctt
    37921 cacctgttgt ttggtgagca tttattcatt acttttattg tgttttacat ttttacgttt
    37981 attgtcgggt tcttattata taaactgaaa aagactggtg tatggcaaaa gctccccttt
    38041 atattgcgat tcttgctggg atcctgattt gcttttctcc gggtaggctc tatgcacaaa
    38101 aagacaccgt aaaggttgga gtttttatta attcgctgca cgatttcaat ttctacgaca
    38161 aaagctacaa aatcgatttc tggctgtggc ttctatatag taatgctgaa ctgagtttcg
    38221 atgaagctat tgaaatccgc ccatcgaagg aattcgacct gagtaataca atacttgact
    38281 caactgcagg tttacagtgg ttaagcatga aagttcgtgc agaaattgtt tatcattggc
    38341 agctcgacca ttatccattc gataatcaga tgctgaacgt agtaattgag tcggctgatg
    38401 ccgataccag cagtatggtg tttatacccg atttcgaaaa cagtaaaatc gacccgaatt
    38461 ttaaactcga cgaatgggat attgacactg tgatagtgaa caatgccatt tcgacttacg
    38521 aaactaccta tggcgatcag gagcttaccg gacagagttc ttaccctgca ataaatattg
    38581 ccgtgtattt gaacagaaaa aactcatggc tttcactgtt taaactgatt acaggggtag
    38641 tggtggcttt ctttatttcg tgcctcgttt ttcttatcaa acccacgaat gttgaccctc
    38701 gttttggtct ctgtgttggt ggtttattcg cagctgttgg gaataaatat ataatcgagg
    38761 gtacggtacc cagtaccaac acaataacca tgctcgacaa cattcacaat cttacatatg
    38821 tgctgatatt actgattatt ctagtgtcta tcttttcatt aaaactattt gagaaaggta
    38881 cagaaaaatc aattaggcaa tcacagcgtg tcgacaggat cagcttgttt gtaataattg
    38941 gaatttattt gctggcaatg gcttacctga ttgttgtcag gtaaaatatt attaatgttt
    39001 ttaaaatgag aaaaatagtg ctgattgttg gtgtattaat atcgctgatg ctgtggtgca
    39061 gcaacagcag tgcccagttt atccagcata attatattga agtcgggaaa accaatgctt
    39121 caggtgggat atatttggat gctgcatcat tatctgcaat tcggtataaa catattcatg
    39181 ctgagttggg ttttctattc ccacttaccg agttcgacaa attcctgagt acattctatg
    39241 caagtgcagg ggtcgatttc aaaataaaaa acctgcctgt tgaaatacat gcattgttta
    39301 cagataaact tgtatcggaa tttatcagcg aattgcagtt cggcataatt gccggcgtta
    39361 aaacaaaaca tgtccacgta tttcttggaa acaacacccg tttatttaac cttacgcgta
    39421 atgcaattga gcaatccagg aacgataatt accgaaagct gcaaattgtt gaaaagctaa
    39481 acttgatgta ttcgcttagc tatcttgttt tccctcagga aaataaatgg aatatcattg
    39541 cttcggtact gaattacgat tatttcctca ttcatcagga gacaaaccca tttgtaagtg
    39601 taaggtttac ctataatcca cgagaaaata taggtttgta tagcgagatc gtgtataaaa
    39661 gtgccggatt taataacatt caggtgaact attttggtgt aaatctctta acgggaatac
    39721 tatgggaaat cgatttttga aaattggatt gttcgcagtt ttttttactg gtgcattgct
    39781 aatgtcatca tgtattggcg atgccgattt tacgggattt tttcgtagct cagtacgtgt
    39841 taatgaacgg tttgagcagt cgcttgaatg gaatacgcag cacgcgccgc gccgcattgt
    39901 ctgtgagaac aataactata ggttattttt tgcttctgac atccatattg gaggcactga
    39961 aaactttcag aagatattaa atgacagtaa aagcgctgaa acggatggat tatttcttgt
    40021 cggtgatatt gtttcaggta aggaagaaga ctataatata cttgtagagt cgcttgcccg
    40081 cgaaagtatt gagaattatt ttatacaggt tggaaatcat gaattgtatt ttaacggctg
    40141 gaaatggttc cggcaatatt tcggtacatc ggtataccct gtggaaatag tcactccttt
    40201 ggcaaaagac cttgtaatat gccttgactc aggaggtggt accctaggtc ctttacagct
    40261 ggaatggctc gaagaattac ttcgaaatgt acgccagaat taccggcatt gcattgtttt
    40321 acatcactgt aattttttcc aaagtgattt cagtacttca gcaacacctt tatcagaaga
    40381 actatacgaa ttaatgaatc ttttctttac ctattcggtc gatatggtaa ttatgggtca
    40441 tgttcacatc agttccgagg aaaattttgg cccgactcgc tatatctgcc ttgatgcagc
    40501 tcttgacgga gccaaaaatg catcatacct gaggcttgat tgtagcgatg accatttggt
    40561 gtatcaatac atttcattgt aggaatcatt ggaatactgt ttacacttaa taattgaccg
    40621 attgctgtat gtttcgtatt attgaagcgt gctcggcttc ttttgaatga ggaaccgtaa
    40681 taaaaacagt aactgagaaa ctaccgtaga aagcagatgt aagataaaag ctatctttcc
    40741 ggttcttgcc tcttatttca taacgattgc cttcgaagtg agaaaaattt atcaaatatt
    40801 ccggatcggc gtaaaatcca acttttacta tgtcctcata accttcctga aatgttccct
    40861 ttgtcagatt atttccaaaa accagtaatt ccatttcacc gctattggat cgaaatgcct
    40921 gcccatcgtt gttgtccggc tctcccagtg gtgtcagaaa atcgggataa tttatgctgt
    40981 atttgaagcg gtcattatag tatttcagat atacgatatt gtcgttgcaa ctgctatcta
    41041 aaacagtttt atcagcggct ggctcaatag aaactgattc aggtcccgtt acctctttca
    41101 ttggttgggt tatgcctccg cacgatatca aggctgaaaa aatgaatgtt attttaatga
    41161 tggtttttgt tctgctcatt tcaaaattca tgagaatagt cgtcaagaag caaatagaat
    41221 gtcggtaatg agaacgaact ggctgatttc aagtttctcg gcacgattat cgagtatttc
    41281 gtcgggtatt tcacggctat ctttacccag agtttttaaa gcattccgaa gagtctttct
    41341 cctttgatta aacgcagctt tcacaacttt gataaacgcc gactcgtcgc agcccaacag
    41401 gattgtattg tttcttcgca atctgatgac ggccgatttt acttttggag gcgggacgaa
    41461 aacggtttcg tttaccgtaa aaaggtattc aatatcaaac caggtttgca ggagaacgct
    41521 tagtatccca tacgttttgc taccggactt ggctgcaatg cgttgtgcta cttctttctg
    41581 aaccatacac actacttcat caatcgaatc acggtttgca atgaggttaa aaaaaatctg
    41641 acttgatatg ttgtaaggga aattacctat gatgcatact ttaggttcga agttttgctt
    41701 gaaatcgagt tttaagaaat caccaagaat tatttttatt tctttatggc tatatttctg
    41761 ctttaatact gcaactgctt ccgggtcgat atcgattaaa gttacgggca cgtttttttc
    41821 ggaaagatat tcgctcagaa ccccggtgcc ggccccaacc tcaataattg gtaaagcttc
    41881 tcggttcagt aagctgtcgg taattttcct tgcaatattt ttatcgataa ggaaatgctg
    41941 cccaagtctt tttttggctc tgatatctgt aatcatatct gcaaaaatac gaatactttt
    42001 atgcaattta cacaaatggt aaagacgggt tttttaaaaa agttatcttt gtcgttctta
    42061 ttcgattagg ttatgaacaa aacgctaaag aatagtattc aatttgtttt attcctgggg
    42121 tttggcgtat tgcttttctg gcttgtttac cgcgatacca actggagcga cttggtagca
    42181 ggactgaaga atgtaaatta ttggtggttc atacccattg ttgttgcagg ccttttcagc
    42241 cacgtcagcc gtgcaatacg ttggaatatg atgattgaac ctctgggtta taaaacccgt
    42301 ttctggaaca cattttttgc agttatgaca gggtattttg cgaatcttgc cattccgagg
    42361 cttggtgaag ttgcccgctg tggagtagtt agcaaatacg ataaggtgcc aatttcgaaa
    42421 gtgctgggta caatgattac agagcgtgtg attgatgtat taagcttatt gctggtagtt
    42481 gtgatagcaa tttttgttga gagtggattt ttttcagact tccttcaaca aaatcctggg
    42541 attggtgaaa aagttgacag tttgttaaac cctcttccaa taattattgc cattgcagca
    42601 gttgcccttt ttattgtttt gttttttctt atactcaaag gcaaattcga caaaataagg
    42661 ctctttttaa agctgaaaac tttagcacga aacctatatg atgggctttt aagtgtgcgt
    42721 aaggtcagaa agaaattctg gtttcttttt cacagtattt ttatctggat tttgtattac
    42781 cttatgatgg cgtttgcttt tccagcattt ggatttacct cgcacattgg tccttcggct
    42841 gccctcatgg tttttgtagc aggtagtttt ggtatgattg cccctgctcc caatggtatg
    42901 ggagcctatc attttatggt aatccaatcg ttgctgattt tcgggattgt taaagaggat
    42961 gcggctgttt ttgcttttgt agttcacggg ctgca
//
LOCUS       DAOOQP010000011        42170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1043_length_42170_cov_5.298374, whole genome shotgun sequence.
ACCESSION   DAOOQP010000011 DAOOQP010000000
VERSION     DAOOQP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 42170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1043_length_42170_cov_5.298374"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="PLK75_02255"
     CDS             <1..1779
                     /locus_tag="PLK75_02255"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY38099.1"
                     /translation="TNSPTSRAWSFPGGTPTSSSATNPTITYNTPGTYNVSLTVTNTY
                     GTDSEVKTGYITVTAAGTAPVANFSGTPTTVVAGNTVAFTDLSTNSPTSRAWSFPGGT
                     PTSSSATNPTITYNTPGTYNVSLTVTNAYGNDSEVKNGYITVTAAGTAPVANFSGTPT
                     TVVAGNTVAFTDLSTNSPTSRAWSFPGGTPTSSSATNPTITYNTPGTYNVSLTVTNAY
                     GNDSEVKNGYITVTTTGTAPVANFVGTPTTVDVGNTVAFTDLSTNSPTSRSWSFPGGT
                     PTSSSATNPTITYNTPGTYNVSLTVTNAYGNDSEVKNGYITVVQPSTNFSLDFEACSD
                     FQVDNFSPWVTLDVDGIPTTNLTGVSFMHSNYTGSFIAFNHTSTNPDASTEYAAHGGE
                     RCGICMPLNSGSNNDWLISPQITLQNGASFAFWAKSASYLGMGTFNVLISTTTNNPAS
                     FSALPQGSNRQGPTVWGEFVYTLSAYAGQTVYVAIQVTSTNGYGLMLDDITLNSSTDI
                     SGQDVFDFMLYPNPNEGSFMIDAGDYNGDLAIEVYNINGQKINVNQSVNGKMLFVDLQ
                     DAANGFYFVRVKSENGIRTFKVSVIR"
     gene            complement(1850..5518)
                     /locus_tag="PLK75_02260"
     CDS             complement(1850..5518)
                     /locus_tag="PLK75_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY38100.1"
                     /translation="MIKVFVNNTEYEAIKGETILDLANRIGIKIPTLCHDPRLAPYSS
                     CFVCVVEIEGMKNLQPSCSTRVMDGMKISTENQRVKIARKTAIDLIMSNHYADCVGPC
                     KIACPAHVDVQGYISLIEKRQYSEAIKLIKEKNPLPAICGRVCVRPCEFNCRRNFLDE
                     GAGVGVDYLKRFAADADLNSDNHYKAPVAPSTGKKIAIIGAGPGGLSAAYWLQQKGHQ
                     CDIYEAMPKPGGWLRYGIPEYRLPNDILDQEITTITELGVRIFCNKKLGENLSFAEIR
                     DEYDSFVLTIGSQKGTLLGTEGEDAEGVYSGIDFLRNMEVTGQKADFSGKTVMVVGGG
                     NTAMDCCRTSIRCKAKKVYVVYRRTEKEMPANPIEIHESKIEGVEYLFLTNPVKVNKT
                     PDGKVESVRLIKMALGEPDASGRRRPVEMPGSEYDMKTDFILAAIGQKTDVNFLEDIN
                     KNLTNGALKITRWGDIEADAISLQTGVPGMFAAGDGVSGPATIIEAVAQAQIASRSCH
                     QYLMGETISKTPDEFVSRKDNFRKLNETEYKGHFEKQHRQEMPVLEAAKRFNFKEVEL
                     GYSSENAALDETQRCLECGCSEFFSCDLQKYATEYGSEQKKYSGSYNNIPVDFRHKLI
                     EIDNNKCILCARCVRICHEMAGANAIGLMKRGFETVVAPAMGESLAKTSCDACGLCIS
                     TCPTGAITENFPFKPGPVKTDSFETISPLGGLGEKITIHHKNDFIFRVTGAKGIVNPD
                     RSIDPLSKFGYRFFNQSQRISRPLLMRNGAFEPISYEEAFSKIVERIKSVEPAENAFF
                     AGARLTNEEQYLVQKLARAGVKTNNIQSFHYLFGGDGHIFNSVKNVSIHELEQAATVY
                     LFSSQLNYDNASIGYVLNKARSAKKTRVELFTTTHNPKLEHKVDKTTRIASLYHFIKA
                     VNYYLLSNGLENGMYIGHHCNDFDLYKAALLKEKYEDLVTKAGSKSDVEKFALAYNTQ
                     MNSVIVFAEKEISGNTATEIRNLAMITGKQGKLASGILSLKEKNNSQGLIDMGVNCCL
                     APGTTDYNSAMIRNTLEKLWGVPVAEPNEKCTGDLLLSGKLKNLFIFGEDPVGCAIDK
                     KHIQAMLKQSDFIVVNDYIITETAAMADLILPASTPFESGGSFTNSHRIIQNFDAGMK
                     PLVDITNAGQINALMLRFGVKNPVSNSEIMAEFSALMDGKFLQQKFCYTAGDNYQRVF
                     AYGCDIVTKYYYEEVAVK"
     gene            complement(5532..7310)
                     /locus_tag="PLK75_02265"
     CDS             complement(5532..7310)
                     /locus_tag="PLK75_02265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOY38101.1"
                     /translation="MEKVKVTVGLGSCGIAAGAGKTYNKLQELQKDEIYNFDLAKTSC
                     IGMCFNEPLVEINNGHETWLYGAVDEKRIVEILEYHTKHNQPLLSYVVFNDKLDLPGK
                     AYFDGQVKIALRNCGYIDPENIEEAEKNGAYTAMKRIASEKITGDSVIQMVLDSGLRG
                     RGGAGFPTGKKWQFAKAYSGDEKYVICNADEGDPGAFMDRSLLEGDPHAVLEGMIISA
                     YSIGATDGVIYCRAEYPLAIIRLNIALEQAREKGYLGKNAFGIQGFNFDIHVKEGAGA
                     FVCGEETALIASVEGKRGMPNKRPPFPAESGLWSKPTNINNVETWANVPWIINNGPEK
                     YAAYGTEKSKGTKVFALTGKIKNGGLVEVPMGITVNEIIYKLGGGIIGDKKFKAVQLG
                     GPSGGCIPAHLGDTLIDYDSLQATGAIMGSGGMVVMDETTCMVDIARFFLDFTVKESC
                     GKCTFCRIGTKRMLEILERICDGKGEDGDIEKLEDLGKRIIENSLCALGQTAPNPVLS
                     TIRYFRDEYESHIYKKKCPAKVCKKLITYQIDETKCTGCTICAVKCPTKAITGEKKKP
                     HFIHQDKCIQCGSCYTNCNFNAISLT"
     gene            complement(7316..7849)
                     /gene="nuoE"
                     /locus_tag="PLK75_02270"
     CDS             complement(7316..7849)
                     /gene="nuoE"
                     /locus_tag="PLK75_02270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOY38102.1"
                     /translation="MRIKRIYADHSQDKQLDALVITNELDEVIGKYKNKKGSIIPILQ
                     STQAIFGYLPRAAFDKIAKETGLEVSDMYGVATFYTQFRLNPVGKNIIKVCHGTACHV
                     QNANAVGEALAEELHIKDGETTPDGLFTLESVACLGCCSLAPVMMVNNNTYGKLDGKS
                     AVKVIREISKKAKTNSN"
     gene            complement(7927..9276)
                     /gene="gdhA"
                     /locus_tag="PLK75_02275"
     CDS             complement(7927..9276)
                     /gene="gdhA"
                     /locus_tag="PLK75_02275"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOY38103.1"
                     /translation="MSKYQQIVDDFMAGVIAKNPAEPEFHQAVREVVESLTPYVEENP
                     KYKNAKILERMVEPERIIIFRIPWMDDKGEIKVNRGYRIEMNSAIGPYKGGMRFHPTV
                     TLGVLKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDAEVMRFCQSLMMELFRHIG
                     PDTDVPAGDIGVGGREIGYMFGMYKKLRNEFTGVLTGKGLEWGGSLIRPEATGYGCVY
                     FAQEMLKTRGLSFEGKMVAVSGSGNVAQYAVEKVNELGGKVVTLSDSEGFIYDPKGID
                     DEKLAYVMHLKNVKRGRIKEYAEKYGCEYHANQRPWIKKCDVALPCATQNEINGAEAE
                     TLVNNGCICVSEGANMPSTPDAIEIYLKNKILFGPGKAANAGGVSTSGLEMSQNSMRL
                     SWTREEVDARLHQIMKDIHATCVKYGTKADGFVNYVDGANIGGFVKVADAMIAHGVV"
     gene            9688..12645
                     /locus_tag="PLK75_02280"
     CDS             9688..12645
                     /locus_tag="PLK75_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOY38104.1"
                     /translation="MKLSIAFLLVLCSSYLYATTYYVTTTADAGNGSLRQAITSANGG
                     PNNTIAFNIPSTDTGFDEQTGVFTIHVSTLLPAIQMINMTIDATTQPGNTNIYGPEIC
                     LMGNESLNYGLLIPTYGNVIKGFIINGFKCGVLIYKTAYLSCNNNTVSDCYLGVNYDG
                     SMSAPNEIGIAIYDDATGNTIRDNLISGNTLAGIGIQSSNTNYIYGNRIGCDRNVSFA
                     IPNYYGVAIDSSSNNIVGGTTMQHRNYISGNTYAGVAINSVLSHNNIVKGNFIGVNSS
                     AIAPSDTLSNYYGVAINESSNNIIGGSTTSERNIISGNLEAGVAILGTSATDNIIIGN
                     YIGPNADGTDSIANGNGILLNGANGTIIGNTSAGGGNVISGNHLSGISMAYSGTENNI
                     VKGNLIGTDKTGMNALSNHTGIYLFSNANNNIIGGTGEGERNIISGNYELGISMEAVS
                     GNIVAGNFIGPDISGTGTFFMSGDISIQGNGLYFNSNSTGNSAVGNLISGNRVYGLIY
                     YGNSSDNSCENNFIGVDITGNTALPNATGICVDGAANHNPITGNVLSGNLAYGIFIVT
                     TGTYYNELKGNKIGTNADGTAAVPNQIGLIIGGGAKQNIIGGSLPEHRNIISGNTFEG
                     IEVADITTMYNTISGNYIGTDITGSQAIPNFNGIGFATRPSHNTINNNVISGNENIGV
                     ILYEFSDSNTVVSNYIGTMADGVTALGNGFAGVVIENSNHNSIGQVAMGNTIAFNGTA
                     GLVVADENSFGNTFSANLIYSNNSMEIDLFPAGVATNDAGDSDSGANMQMNYPEITQA
                     AYDPVEGIALIEGTIDNTVNGSAGGIRIEVFRSNESNPFQHGGADLYLGSGYANASGE
                     WHVWAPNLTDSDKITATATDTEGNTSEFSLNSDVFVASPEILYRTNEFTIFPNPVNEN
                     VWISSDKWGNYPYRFTLTTSDGRLVWTKECLRIDKSIMLELGENVIPSGMYFLSIYKD
                     GIITTTKKIAVIK"
     gene            12642..14687
                     /locus_tag="PLK75_02285"
     CDS             12642..14687
                     /locus_tag="PLK75_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOY38105.1"
                     /translation="MKFYCAYIKTLVLFIVLLIGNQAIANTFIVTNTADSGNGSLRWA
                     IQNCNIYPGSHTILFNIPESDGNYNEAQGVWRIDLTSNLPMLNHSNVLIDGHSQATYS
                     GDKNPYGPEIVLDGGNNDWADYGINIYNASGITIQGLIIGGFVVGIQMTGENAFNNTI
                     SGNYIGCNYNATESNGNTHGIYILSGPHNNIIGGNTPVARNILSGNNHTGMRVVNASY
                     NTIQGNYVGLNRTGTAALHNYDGISIEGISQYNLIGGYTPEERNYVSGNMNYGIPAFG
                     IGCYYNIIAGNYVGTDITGNNAIPNTYGVLFDDGACYNTLGGITEGAGNLLSGNSGYG
                     VFIYNFGTMKDTVRGNLIGTNAEGTAAVPNANGIVIDGPSYQHTIDNNVISGNLQMGI
                     DIHIAGSDSNIVINNKIGTDISGMNPLGNLIDGIRIAEGPDNNIIGRPGQGNIIAYNG
                     GNGVTIMTAAEQANTISGNSIYANEGLGIDLFPEGITANDEGDSDSGPNSNMNFPVIT
                     QISISTNVQLWGHVDSPGADEITVEFFKSDYYPSGTGQGREYIGSCSTDASGNFSYSI
                     AGGLSGAIYICTTATDGSGNTSEFSANKLCVQTGIDQHVEVTEPVSIYPNPAATSFRF
                     NNFEIGRRFLIVNNSGQVMGSGIWKGDNIPVSYLLPGSYYVLPTDVAAHVILLIIMR"
     gene            14823..16175
                     /locus_tag="PLK75_02290"
     CDS             14823..16175
                     /locus_tag="PLK75_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOY38106.1"
                     /translation="MKIHLIACGGAVMHNLAIALHKKGYTVTGSDDEIFEPARSNLAK
                     YRLLPEAWGWFPDKITSDLHAVILGMHARRDNPELVKALQSGVKVYSFPEYLYEQTKN
                     KTRIVVGGSHGKTSVTSMIMHVLNRCGMHFDYMVGAKIDGFDTMVNIEEANEIAIFEG
                     DEYLTSCIDPRPKFHWYKPHIAVLTGIAWDHINVFPTFDDYIRQFSMFVDIIDETGCL
                     IYFKNDKNLQTLVEEKTRLRCMPYYELDSEISDNKTIIKLRNNTYETKLFGKHNMQNI
                     NAARLVCNEIGINNEQFFAALSDFKGAAKRLQLVAENKSSAFYIDFAHAPSKLKATTE
                     AVKQRYSGRKLVACIELHTFSSLSKSFLPQYNGSMEMADTAIVYYNTHVLEHKNLENI
                     DPEMVKQAFGNDVLVFTDSLLLQKFLLQTDWDNTNLLMMSSGNFDGINFDMFAKQIIR
                     "
     gene            16189..16683
                     /locus_tag="PLK75_02295"
     CDS             16189..16683
                     /locus_tag="PLK75_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOY38107.1"
                     /translation="MLLKIITLLNHHFPYKAILAIILVGMLTGAQSCKYQEITIGTPN
                     GLRVKDISLEKVNLQIDIPINNPNNFSFNISKTELEVYIAGVKIGTITKFEKTKIDAR
                     SNEVYPVGFSFKPTDILGNSIKLVSELSKRNTEVEVKGKIRVSKFIIAKNIKVHSKET
                     VKIY"
     gene            16706..17149
                     /locus_tag="PLK75_02300"
     CDS             16706..17149
                     /locus_tag="PLK75_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38108.1"
                     /translation="MRTLIIGLITFLFSGTLFSQSVTPVDIPYFNVGAYGGKIQIHQS
                     DKIENFYETYVGINKKRNGFYGYRVKIFADVGPTARKEANDIKVRFNSSYPEYKAYLI
                     YNEPNFEVHCGNFRTRIEAMALLSKIKNAYPGSFIIYEIIEFPNL"
     gene            17158..17976
                     /locus_tag="PLK75_02305"
     CDS             17158..17976
                     /locus_tag="PLK75_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOY38109.1"
                     /translation="MPDISNLIQKAAEIRRDVIKSLNKAGSGHLGGALGLADVFTVLY
                     FYELRHNSGNAEWHARDRVVLSIGHVAPVLYTTLAHAGYFHESELGTLRKEGSRLQGH
                     PSLQHKLPGVETSSGSLGQGLSIAVGMALAMKIEKSIRNIYCILGDGELQEGQIWEAA
                     MSASHHKLDNLLAIVDRNEVQIDGSTRAVMDLEPLTQKWESFGWNVIVCRGNDISDLI
                     RTFDQIRNIRNKPSVIIARTTMGKGIRSIEGDYTWHGKVPDNEQCSAFLKELGF"
     gene            18118..18516
                     /locus_tag="PLK75_02310"
     CDS             18118..18516
                     /locus_tag="PLK75_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38110.1"
                     /translation="MKADYICPKCHGHLKVKTSIILLTKNSKGDSALVLLSPEIGDYS
                     FTIHPSVKIAEGEHTNFICPICYENLNTDDLDRNLAKLIRIDENNHRSEVVFSQIKGE
                     KCTFKINEGGIEAFGDDTRNYLNHFGLQSE"
     gene            complement(18519..19559)
                     /gene="selD"
                     /locus_tag="PLK75_02315"
     CDS             complement(18519..19559)
                     /gene="selD"
                     /locus_tag="PLK75_02315"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOY38111.1"
                     /translation="MKTDLLSFCESGGCSAKISAKDLEGILAQIPMAVHRDLLVGAST
                     GDDAAIFRLNSETALIFTTDFFPPVCSDAYTFGKIAAVNALSDIYAMGGKPLMALNLV
                     MFPAVGIELTELHEILKGGNDAAAAADCIITGGHTISDSIPKYGLAVVGLVHPGNIIT
                     NAAAEPGDDLILTKPLGTGIALAAHKTGIAETTVLDEAIRSMCLLNKTGSEIMQKYHI
                     RAATDVTGFGLAGHALKMAIASNVSMKIYTESLPYFTGINQFVERGCIPGAVFRNMKS
                     TENEIFIPWRFDYFYNVIIHDAQTSGGLLISCPAKNTKQMILDFQTDPRFGKTAVIGK
                     VLARQPKRIYLE"
     gene            complement(19561..19779)
                     /locus_tag="PLK75_02320"
     CDS             complement(19561..19779)
                     /locus_tag="PLK75_02320"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOY38112.1"
                     /translation="MHEKYYILFRSTRDVILAEKLAKSQFFQFTIVPVPKHISAECGL
                     CINADVRTAIQLYEFLSEHGLKCEKLEM"
     gene            complement(19772..20899)
                     /locus_tag="PLK75_02325"
     CDS             complement(19772..20899)
                     /locus_tag="PLK75_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY38113.1"
                     /translation="MNVYFDNSATSWPKPPEVAQAMTSFLHELGTSYGRGAYKRVLMS
                     TGIVEECRDALAAIMHTELRENIVFTLNATHAINTVLKTLPLKGKKVLVSPMEHNAVV
                     RPLQYMKDNALITDFGIIPALKNGVIDIQALQTMLSADIGLVCINHASNVNGVVQDIA
                     AIKRISGNIPMLVDASQSLGSYDVFLDDWKVDYLCFTGHKALMGPTGTGGFFVREPAT
                     MNTFIHGGTGSNSASFQMPDFLPDKFEAGTHNIVGIAGLLAAINHKPTPNHSIIELED
                     LISKISELQGYRLYTSNHPGSHSAVFSLVHSKLSSSELSERLFQQSGIETRSGLLCAP
                     LAHTTLQTFPQGTCRISISPYHTRHDFDYLYTALKKIAAHA"
     gene            complement(20901..21497)
                     /gene="yedF"
                     /locus_tag="PLK75_02330"
     CDS             complement(20901..21497)
                     /gene="yedF"
                     /locus_tag="PLK75_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOY38114.1"
                     /translation="MITINARGLACPQPLILAKQALDKAAVGESICISTDSETSMQNL
                     MTFLRDLKAQPVCAKKDDTFFVTAKKSATQTNSSETSCSINSYRSHVVAVTSETMGSG
                     PEDLGRILMKAFINTLNEAENLPSHLVFYNSGVLLTHCESDVLGSLKALENKGVYIHI
                     CGTCADYFQIRDKIGIGQVSNMYTIVEVLSKATSIIRP"
     gene            complement(21494..22525)
                     /gene="mnmH"
                     /locus_tag="PLK75_02335"
     CDS             complement(21494..22525)
                     /gene="mnmH"
                     /locus_tag="PLK75_02335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOY38115.1"
                     /translation="MQNEIDINEFLLLAEQMIVLDVRTPLEFEAGHIPGAVNFPLMSN
                     EERVIIGTIYKQSGKFSAVLKAYQLLGPEMSNMLKTAQKLLPGKKAIIYCWRGGMRSE
                     SMTWLLHSAGFEVKRLSGGYKAYRNYIRQLFAQVNKLCIVGGKTGTGKTEILFELKQR
                     GEQVVDLEGIASHKGSAFGHIGMKPQSTNEQFENNLAEVIKCFDKKKRIWIEDESKSI
                     GKNFIPDELWQKMFQSPLFFVESDKEFRLDRLVRDYSEADNSALHEALGKISEKLGGD
                     NFKKATKALVEGDLKLFAELTLVYYDKAYLHSLGKHPADTIHRIDVQRLSASEVANQL
                     INKANILGL"
     gene            complement(22614..23984)
                     /locus_tag="PLK75_02340"
     CDS             complement(22614..23984)
                     /locus_tag="PLK75_02340"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOY38116.1"
                     /translation="MSTTKNYIEANKERFLSELFGLIRIPSISSLSEHKNDMIRAAEY
                     WKQMLLQAGADKADLYNTDGNPVVFGEKYVSPKLPTILVYAHYDVMPVDPVDKWETKP
                     FEPVIKDGKIWARGADDDKGQGFMHAKAFEIMVKTGTLHCNVKFMIEGEEEIGSPSLT
                     KWCAENKEMLGADVILVSDTGMIGPGIPTITTGLRGLSYWEVEVTGPCRDLHSGLFGG
                     AVANPVNVLCNMIAQMQDENGKVTIPGFYDDVLEVSRKERDLYALAPYDEEEYKKALK
                     VKALKGEKGYTTTERTGIRPSFDLCGIWGGYTGEGAKTVLPSKAYAKVSTRLVPNQDH
                     HKISELFAKHFKSIAPDYVSVKVTPLHGGQGYACPVDHPAYKAAEKALFDTFGKQAVP
                     FRSGGSIPIISTFEHVLGIKSILLGFGLESDAIHSPNENFPLEHFFKGIETIPLFYKY
                     YAEMVK"
     gene            complement(24000..24635)
                     /locus_tag="PLK75_02345"
     CDS             complement(24000..24635)
                     /locus_tag="PLK75_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38117.1"
                     /translation="MPWLLKYYILLIAVGALPVLLNAQVQPRDTRPTDGLSKWDKMFP
                     QIKVGDKVFKTGSNWFTAGVGYGYATNLRKQQTNFALGYHHRYHAMYFNLGYHYSSDQ
                     FFLKRPMVRLNDVHLGAGLRIEKIHFNFAFFIGPTWSFGSLYNYTDPYGTVYYTYFHT
                     LGAHTELQFTWKFFYDLGIGTSLYGSFNKQYQVVGVQLHFYFSSAFKPPRK"
     gene            complement(24683..25273)
                     /locus_tag="PLK75_02350"
     CDS             complement(24683..25273)
                     /locus_tag="PLK75_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOY38118.1"
                     /translation="MIEIEKTLVSFDIIDKRFACNPGLCKGICCVQGDSGAPLEDSEK
                     YEIEQCLPEVTSILTAEARNRIQNSGVAVIDTEGDLVTNTLHGKGACVFAIRNSQGIT
                     ICALELVWTQGKTQFRKPLSCHLYPVRITRYKYYDAVNYHTWDICKSAIETGENKGIP
                     LYVFLKDAFIRKYGEEWYNQLVYAAENIKTAVSLKK"
     gene            complement(25278..27713)
                     /locus_tag="PLK75_02355"
     CDS             complement(25278..27713)
                     /locus_tag="PLK75_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOY38119.1"
                     /translation="MYYPEDFEHKIGFRRIREMLAHRCKGTTAKELCGTGLVLADIDE
                     IKRKLAATDEMKTILLLHSELSITEYPDISHLLKQLCVDGIILAQEEFVDLFNVLSQS
                     KNVLNYFKKANKDTCRNLYSFVEGVVFPQFVYDRIDFVFSRDGRIKDKASKTLFEVRS
                     SLISNKKLISSKLNNLLETFHKEGWLSSDLSATIVNGRTVLPIETTHKRKIQGLIHDE
                     SASGKTSYIEPAEVVGMNNRQRELELEEQREIRRILKELTADIIPYKSDISGLFGMLV
                     ILDFIRAKAILAIEMDAIRPEVHDDMNIELHEAKNPLLLMAFKNENRRVIPLNLSLNE
                     KQRIMLISGPNAGGKSVAMKTVALLQYMTQCGMLIPCGGNTSMGMAKKMFIEMGDEQS
                     IENDLSTYSSHLLNMKFFLKNACERTLIFIDELGSGTEPVQGGAIAEAILKQLLEKKC
                     CGIVTTHYTNLKAFASATEGIENGAMLIDNNLMKPTFQFQAGLPGNSYALEIADRIGL
                     PQAIIIDAKEKAGTGLTKLESLMRKTLRDRRYYERKRLVVRQQEKQLEQKMHEYEEKI
                     RKLNAERKKILEESKIKAGQILKDMNSQIESTIRIIREINADKEKTRKLRQELESYVA
                     EKNDEFLKIDSDSPVEEEFKQYANKELEFVDIDPEEIHIGSKVRLAGHETIGEVVDMG
                     SENAVVQFDSVITSVAKSKLEKVPDNETSPKTRISAGSLYQNINELRSTFKPSIDVRG
                     KRAEEALSEITVFVDTAIVLGVSQLKILHGKGNGVLRKAIREQLKSVKEVAYFSDEDI
                     RFGGDGITVVQMK"
     gene            27761..28180
                     /locus_tag="PLK75_02360"
     CDS             27761..28180
                     /locus_tag="PLK75_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY38120.1"
                     /translation="MRKITLLLIITGIVNLLYSQNDTIDSQNMDINYTREASYPGGMN
                     KLIGDIWNSMEFTQEAIDKKIDETLMVSFDVNRDSTISGIILLNNIGYGVDEEFIRVL
                     GKYKFLPALAQGNPIKQNVIISIPIRTGPNSKKKSNN"
     gene            28413..29147
                     /locus_tag="PLK75_02365"
     CDS             28413..29147
                     /locus_tag="PLK75_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY38121.1"
                     /translation="MLKRLTIILLFICIILQVSGQRRHPENLPKLDQQLAHFGYSLGL
                     NSMSFTIRPSAEYFLMDTVYAIETGHFVGFNINMIANLRLLEYLDVRFLPGLIFGQRN
                     LEYKILEDSIFRRHTMMIESTFLDFPIVFKYKAARLNNFRPFVIAGGAFRVDLAAQRK
                     IDPEEMPKIKLSPLDTYYEIGLGADFFLEYFMFGIELKGSFGIFNMVKYDNTEYTRAY
                     SRLNSKMLILSFHFEGGKIDKIKWWD"
     gene            29152..30708
                     /locus_tag="PLK75_02370"
     CDS             29152..30708
                     /locus_tag="PLK75_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOY38122.1"
                     /translation="MNPRVVEIQMQLPPEEAYNIGTIKSAVAKESGIDISRISQIRIS
                     KRSIDARRQVKVQFTVSYTLDGELLPELAGQWEFKDVRVSDKQVIIAGSGPAGLFAAL
                     RLLEAGICPVIIERGKAVDDRNKDIALLNRNEHFDPESNYAFGEGGAGTYSDGKLYTR
                     STKRGDSNRILQLLHHFGAGETVFTDTHAHLGTDQLPKIIKAIRQEIENHGGRFIYST
                     RITDLVIKNGKVCGITTHLGESIVCDALLWSTGHSASDNYMMLQQHGIAMQEKGFAMG
                     IRIEHPQELINEIQYKKYSHSTFLSPATYFISQQVENRGVYSFCMCPGGQIVNASTAT
                     DSMVVNGMSNSKRNSRFANSGLVVEIRPEDVPGNDIFRLLHYQQELERLARTNGGPPM
                     KAPAQALADFAAGNISKNLPQTSYLPGVISSPVHFWLPAAISLRLQQSLRYFGKFMHG
                     FVSNEAIALGVESRTSSPVRILRNNETMQSVSLEAFFPCGEGAGYAGGIVSSAVDGDM
                     AAQKIAGYLN"
     gene            30715..31392
                     /gene="trmD"
                     /locus_tag="PLK75_02375"
     CDS             30715..31392
                     /gene="trmD"
                     /locus_tag="PLK75_02375"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOY38123.1"
                     /translation="MRIDIITVLPELLESPLNHSIISRAVKKGLAEIHVHDLREYGIG
                     KYRQVDDYVYGGEAGMVLMIEPVFKMIEKLKSDRSYDAIIYTSPDGKPWKQKTANHFS
                     TFSNILILCGHYKGIDHRIREHLITHEFSIGDYVLTGGEFAAIVFVDSMVRLLPGAIS
                     DEQSALSDSFQDDLLAPPVYTRPAEFNGWKVPDILLSGNPTEIEKWKDQQALERTQQL
                     RPDLLED"
     gene            31548..32762
                     /locus_tag="PLK75_02380"
     CDS             31548..32762
                     /locus_tag="PLK75_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38124.1"
                     /translation="MKVRLLVFIAVLAVLWGCNHENEPQIYTVSGKVQKGPFTTGTNI
                     ILNELTTSLAQTGRAFTTTMSTNDGSFSLGNIELESELALVTATGYYFNELYGELSPA
                     TLTLSAITNLSANGTVNINVMTHVIRARTEVLVSTGMSFRDASEQAQSEFLAFLNAAD
                     SGSQSFDDLDISQNNSYNASLLAFSVILQRYTNKALEKPQLTAELTQLLANISTDFAP
                     DGQITNQAIIDTLLYNISNLNLYDIRENIAQHFAGLGITADIPDFETYVDKFQELHSH
                     YLYQDFYYPTNASPEPIVFPHDEVPNLLALNVVNFTSQATYCIAAVVPLYSSLRIQIT
                     FTSNPSGFTVTPGTHGWHTERDGNVLNMYSQRRNTLMSYMVGFEGTGSALIEYFENGE
                     TSPSFTKTITWQ"
     gene            complement(33043..33873)
                     /gene="ispE"
                     /locus_tag="PLK75_02385"
     CDS             complement(33043..33873)
                     /gene="ispE"
                     /locus_tag="PLK75_02385"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOY38125.1"
                     /translation="MICYPNAKINIGLHINCKRPDGYHDIETLMLPVPLCDILEILPV
                     GNAKVPFEMGVSGIPLDCDSGDNLCRKAFDMLCLHHKLPPVKVHLHKIIPSGAGLGGG
                     SSDAAFTIRMLNDMFALKLTHEQMISYASSIGSDCAFFIENTAAVASGRGEILVDYPY
                     DISEYFLVLVLPDVHVSTAEAYKNCAPYLREKSIVTIASRPIHEWKNTLINDFERSVF
                     NAYPVLEEIKKELYSMGAVYASMSGSGSAMYGLYTDLPETGKLKSQYKLYVFRNNKQI
                     "
     gene            33951..35129
                     /locus_tag="PLK75_02390"
     CDS             33951..35129
                     /locus_tag="PLK75_02390"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOY38126.1"
                     /translation="MPSSVIRYYFKEDYSFMQSTLRLLAWFGSIGHLLFYFVLLYLVG
                     YWESLPVRIAASALFLSLLLLPKLRKFNTFHIVYYEFVYTLVFPVMFLFFLVQNNLNA
                     YWAASVLFSAVLYGFLTHPPKALLMYPLSIAVTLLVLVKLNMLNQPVSEVILIHFPAY
                     FIMILIGILQTQIRYANTKAEKENARSESLLRNILPEKIINTLKNNPENIAEKYEETS
                     ILFADIAGFTPFTEKSKPEEVVEFLNDIFTRFDALSEKYKIEKIKTIGDAYMVVSGAP
                     VACANHAEVIADMALDMLAEIKEYNASHSSSISIRIGINSGPVVAGVIGKKKFAYDIW
                     GDTVNTASRMESNGIPGKIQITGATAALLNGKFLVSFRGELDIKGKGVMKTYFLEGKN
                     "
     gene            complement(35152..36408)
                     /locus_tag="PLK75_02395"
     CDS             complement(35152..36408)
                     /locus_tag="PLK75_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY38127.1"
                     /translation="MNWLRHAYIIFVPIILLTNNVLLVAQNTVLLKGSIYDIETGNPV
                     NGVNIYNINLRNGATTAASGAYSIRVNEGDTLVFSHVGYVKQQQVITSGVFTMDVAFE
                     PSVTILPAAEVRPVVKISYGMMLDVGDYAFIGDSILYYGYCYRYDKNRNPWLVLVNGQ
                     GDTVATFCAGADGRLFRDCMGNLHFLTAATAFQLSFDTDSIVLLYPSPKKEFLEIVQP
                     CIIQSGSKFLFSEYSDRNQILMYYYADTLSQSYEMLRTIVDEVKHEMFVFHGVFLSMG
                     NCNGPSDADLRFEDMMFDSVFAPAIKLRDTIALINYTDSVIEWYNNDFHFLGKVGIQF
                     QNNRYIENQILTDEVTGNVYAVYLKNGKTKLRQIYIHSGTLGKEISIPDFAWVGNLKV
                     HNGQLYFLYREKFTGDFQALYKMGLQ"
     gene            complement(36454..37521)
                     /locus_tag="PLK75_02400"
     CDS             complement(36454..37521)
                     /locus_tag="PLK75_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38128.1"
                     /translation="MNNKYYHTEIIESYLKGTMNADEKAAFEKALTEDEFLKDAIAGF
                     QQSGALPEDVEIISMPSKVKPTLKRLTKVLLYSGIAAAVVAVFIIIHVLSGKAVPPKN
                     TNHGNELANNTGETRFVIPVVDTVAPWVEAETIYTGEMLQLPKESSHSESIEPLFVNK
                     SMVIQQTTKEPDIVDLYRFRSNHPYTYICGLKVVDYRFEIRRQPNSNNIPANKVQADY
                     LNPIEFAGADATYTYVAFLEQSLGFYKQGRYQEAIENFIIIEEHFPGDLNAIFYKAMC
                     YYNLNNNKKSIESFTALLNADINTFIEESMWYSSLIYKEEKQYAAAEKVLEEIVKENG
                     YYGAQAQKELDALYDEYLDGK"
     gene            complement(37514..38056)
                     /locus_tag="PLK75_02405"
     CDS             complement(37514..38056)
                     /locus_tag="PLK75_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY38129.1"
                     /translation="MLRNLSDQDLLKMLGSKQSDMVFAEFYRRYSHMVYGVCLKYFNS
                     RDESLDAVIEIFEMLMSKLHLHVITNFSSWLYSVTKNHCLMKLRKEKREQLAIDNIQI
                     HESVNAETDETESEHHQKLAAAIERLSSEQKSCIMLFYFNRKSYTEIAGETGYSVKMV
                     KSHLQNGRIKLKKLIEKNYE"
     gene            complement(38086..38805)
                     /locus_tag="PLK75_02410"
     CDS             complement(38086..38805)
                     /locus_tag="PLK75_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOY38130.1"
                     /translation="MKTIIILAILLISAGSLLAQGSAIHGRVLDENGKPMPMVNVVVK
                     TGSSIAGTSSDFDGEYKLKPLDAGTYTVEFSFVGYSVTEVTGVTVYNSQITFLDDVTM
                     LIKPEMKKEVVIYGDKLIKKDNPSLIPIPSATIVKLPGPKNPIMVARQICPEIQIYEN
                     KMVVRGSRPGSSSVFIDGVKVRDEMSSIPSLAIGSMEIYTGGIPSKYGDVTGGVVMMT
                     TKGYFDIMNERKAVESKTDDF"
     gene            complement(38925..39368)
                     /locus_tag="PLK75_02415"
     CDS             complement(38925..39368)
                     /locus_tag="PLK75_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOY38131.1"
                     /translation="MADPIVKKYKNDDITVIWQPHLCIHSTVCFAKLPLVFRPSKRPW
                     VDLSKATTEAIIETVDMCPTDALTWEWNSPDGNVSDQEMANSSGIKARIIKNGPLILP
                     KEVRLFDSNGNEVYAGKPKSICRCGKSKKMPGCDGSHKQINFEDC"
     gene            39520..39960
                     /locus_tag="PLK75_02420"
     CDS             39520..39960
                     /locus_tag="PLK75_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38132.1"
                     /translation="MIYKKIYSLLFLMLTLAGSVGFFAVQHYCNSCMHSGSELQLFSV
                     SDSHHHSCKSCEDSGHNCDCHSITKKGQCSSLFFLIPVFSNTSPNTFKNIQLVTSFSI
                     KAFDVSAKLLKEPEPLEIFFNRQKIPIFLTFNSRSITSTGSFRL"
     gene            40016..>42170
                     /locus_tag="PLK75_02425"
     CDS             40016..>42170
                     /locus_tag="PLK75_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38133.1"
                     /translation="MKLKTILLTPSFIILYLFAQAQVTGKVYYKEGQKSVPLIGANVY
                     WQGTQEGTATDNNGSFSTEKPASTNVLLISFVGYGTDTIVVNENNRYLEIILTKTVLL
                     DEVTIGERNSSSTYARLDPIGTQQLTGEELKKAACCNLGESFETNASVDANYNDASTG
                     AKQIKLLGLSGKYVQMLTENIPNFYGLATSYGMEYIPGSWMNSIAISKGTASVSYGYE
                     AITGQINTWYKEPDKKADYFYLNLLGNTAGKTEINMDGSFNISKKVSSMILIHGGYDF
                     FQVDHNNDGFMDMPSVKKYILFNRYNIHWSDDFETKIGVKYLFDDRTGGQLPDMVNPY
                     QILIRTNRLEAFLKSGYFFANKSAGSVAFISGYSYHNQNGVYGNKLYNAFQNSIYTNV
                     IYSTELGSSEIHSFQSGASYKYDAFNENLNDSVSLRQELVPGVFAEYTLKPSAAFTGV
                     LGMRVDYHNLHGFFYTPRMHIRYKVTGTTTLRATAGSGTRTANALAENSYLLASSRRI
                     VFSEELKPEKAWNIGGSVIQEFPIGNRTLTLIADFYRTWFINQVVIDMDTDVHEVNFG
                     NLKGNSYSNVGQIEVQYPFIKGMDFTAAFRYNDVKQTIGDILMKVPFTSRYKGLLSLS
                     YKTPLKKWQFDITSQFNGGGRIPSTAQNPVYYQRADHFDPYTILNAQITKYFKVWEIY
                     AGVENLTNFVQHDPIIASENPWGESFDGAMIWGPVH"
BASE COUNT        12202 a         9049 c         8350 g        12569 t
ORIGIN      
        1 acaaattcgc caacatcaag agcttggtca ttccctggag gaacacctac atcttcgtcg
       61 gctacaaatc caacaattac ctacaacaca ccgggtacct ataacgtaag tctgacggtt
      121 accaatactt atggtactga cagtgaagtt aaaaccgggt atattacagt tactgctgcc
      181 ggaacagccc ccgttgccaa tttcagcgga acaccgacta cggttgttgc aggaaataca
      241 gtagctttca ccgatctttc aacaaattcg ccaacatcaa gagcttggtc attccccgga
      301 ggaacaccta catcttcgtc ggctacaaac ccaacaatta cttacaacac accgggtaca
      361 tacaatgtaa gtttaaccgt taccaatgct tacggcaacg attcagaggt taaaaacgga
      421 tacattactg taactgctgc cggaacagct cccgttgcca atttcagtgg aacacctacc
      481 acggttgttg caggaaacac agtagccttc acagatcttt ctacaaattc gccaacatca
      541 agagcttggt cattccccgg agggacacct acatcatcgt cggctacaaa tccaacaatt
      601 acctacaaca caccgggtac atacaatgta agtttaaccg ttaccaatgc ttacggcaac
      661 gactctgagg ttaaaaacgg atatattact gtaactacaa ccggaacagc ccccgttgcc
      721 aatttcgttg gaactcctac taccgtagat gttggcaata ctgttgcttt caccgattta
      781 tcgacgaatt caccaacgtc acgctcatgg tcattcccgg gagggacacc tacatcatcg
      841 tcggctacaa atccaacaat tacctacaac acaccgggta catacaatgt aagtttaacc
      901 gttaccaatg cttacggcaa cgactctgag gttaaaaacg gatatattac agtggtacaa
      961 ccatcaacta atttcagcct cgattttgaa gcctgcagcg attttcaggt tgacaatttt
     1021 agtccatggg taacccttga tgttgatggg atacccacca caaacctaac cggagtctcg
     1081 ttcatgcatt caaattatac aggttcattc attgctttta atcatacttc aaccaatccg
     1141 gatgccagca ctgagtacgc ggctcacggt ggagaacgtt gcggtatttg tatgccttta
     1201 aactcgggca gcaacaacga ttggcttatt tctccgcaaa ttaccctgca aaatggtgca
     1261 agcttcgcat tttgggctaa atctgcatca tacttaggta tgggcacatt caatgtctta
     1321 attagtacca ctacaaacaa cccggccagt ttttcagctc taccgcaagg aagcaaccga
     1381 cagggaccga ctgtatgggg tgaatttgtc tataccctaa gcgcttatgc agggcaaacg
     1441 gtttatgttg ctattcaggt aacctcaaca aatggatatg gtttgatgct ggatgatatt
     1501 acactgaact catctaccga catctctgga caggatgtat ttgatttcat gctatatcca
     1561 aaccccaacg aaggtagctt catgattgat gccggagact acaacggaga tctcgcgatt
     1621 gaagtttaca acattaatgg tcagaagatt aatgtcaatc agtctgttaa tggcaaaatg
     1681 ctttttgttg acttgcagga tgcagcaaac ggattttact ttgtaagagt aaaatccgaa
     1741 aacggaataa gaacatttaa agtttcggta attagatagt tcttcaccaa taaaaaagaa
     1801 gaggctgccc taacggagca gcctcttctt tttaggtact tggggttaat tatttaactg
     1861 cgacttcttc gtaataatat tttgtaacga tatcgcaacc atacgcaaac acccgttgat
     1921 aattgtctcc ggcagtatag cagaacttct gctgaagaaa cttgccatcc atcagagcag
     1981 agaattctgc cattatttcg gaattcgaga caggattctt caccccgaat cggagcatta
     2041 gtgcatttat ctgtccagca tttgtgatat caacaagcgg tttcattcca gcatcgaagt
     2101 tttgaattat tctatgtgag tttgtaaaac ttcccccact ttcaaaagga gtactggcag
     2161 gcaatatcaa atctgccatt gccgctgttt cggtaattat gtaatcgtta acaacaataa
     2221 aatccgattg tttcagcatc gcttgtatat gcttcttatc gatagcgcaa cccacaggat
     2281 cttctccgaa aatgaatagg tttttcaatt taccggaaag cagtaaatcg cctgtacatt
     2341 tttcgttagg ctctgccaca ggcactcccc aaagcttttc cagtgtattc cgaatcatag
     2401 ccgaattgta atcggtagtt ccgggagcca ggcagcagtt cactcccata tcaatcaaac
     2461 cctgcgaatt gttcttttct ttcaaactaa gaatgccgga tgcaagtttt ccttgttttc
     2521 cggttatcat tgcaaggttt cttatttcgg tagcagtatt gcctgatatt tctttttcgg
     2581 caaatacgat aaccgagttc atttgcgtat tatatgcaag tgcaaatttt tcgacatccg
     2641 atttgctacc ggcttttgtt accagatctt cgtatttctc tttaagcaat gctgctttat
     2701 acaggtcaaa gtcgttgcaa tgatgtccga tatacattcc attttcgagg ccattgctca
     2761 acaaataata attaacagcc ttaatgaaat gatataatga tgcaattcgg gttgttttat
     2821 cgactttatg ttccagtttg ggattatggg tagtggtaaa cagttcaacc ctggtcttct
     2881 tagcactcct tgctttattc agcacataac ctatgctggc attatcataa ttcagctgcg
     2941 aggaaaacag gtaaaccgta gccgcttgct ccagctcatg aatactaaca ttctttacag
     3001 agttaaaaat atgtccatcg cctccgaata aataatgaaa gctttgaata ttattagttt
     3061 taactccggc acgtgctagc ttctgaacca gatattgttc ctcattcgtg aggcgtgctc
     3121 ctgcaaaaaa tgcattctca gcaggttcaa ccgatttaat cctctcaaca attttactaa
     3181 aagcctcctc ataagatatg ggttcaaaag ctccgtttct cataagaagt ggtcgtgaaa
     3241 tacgttgcga ttgattgaaa aaccggtatc cgaatttcga aagtggatcg atgcttctat
     3301 ccgggttaac tatgcctttt gctcctgtaa ccctgaatat gaaatcattt ttatggtgaa
     3361 ttgtaatttt ttcgcctagg ccacccaatg gacttattgt ttcgaatgaa tcagttttca
     3421 cagggcccgg tttgaacggg aagttttcgg tgatagcacc tgttgggcat gttgatatac
     3481 ataacccgca ggcatcgcac gatgttttag ccaatgattc tcccatagca ggagcgacta
     3541 cagtttcgaa acctcttttc atcagtccga ttgcattggc tccggccatt tcgtgacaaa
     3601 ttctcacgca acgggcgcac agtatacatt tattgttatc tatttcaatt agcttatgcc
     3661 tgaaatcgac aggaatgttg ttgtagcttc cagagtattt cttttgttcc gagccatatt
     3721 cagtggcata tttttgcaaa tcgcaggaaa agaattcact gcaaccacat tcaaggcaac
     3781 gttgtgtttc gtcgagagca gcattttcgc tggagtaacc caactcgacc tctttaaaat
     3841 taaaacgctt agcggcttca agcaccggca tttcctgacg gtgttgtttc tcgaaatgtc
     3901 ctttatattc cgtttcgttg agtttgcgga agttatcctt tctgcttaca aattcatccg
     3961 gtgtttttga gatggtttca cccataaggt actgatggca gctccgtgaa gcaatctgag
     4021 cctgagcaac agcttcgatt attgtagccg gtccactcac accatcaccc gctgcaaaca
     4081 ttccgggaac gcctgtctgt aacgaaatag catccgcctc aatatcgccc cagcgagtta
     4141 ttttcaaagc tccgtttgta aggtttttat ttatatcctc aaggaaattc acatctgttt
     4201 tctgaccaat ggcagcaaga ataaaatcgg ttttcatatc gtattccgac cccggcattt
     4261 caacagggcg tcttctaccc gaagcatcag gttctcccag agccatttta atcaggcgca
     4321 cagattctac tttaccatca ggcgttttat tcacttttac cggattggtt aaaaacaaat
     4381 attcaactcc ttcaattttt gattcatgaa tttcgattgg gttagcaggc atttcttttt
     4441 cagtacgtct gtaaaccaca tagactttct ttgccttgca tctaatcgat gttctgcagc
     4501 aatccattgc tgtatttccc ccgccaacca ccatcactgt ttttccagaa aaatcggcct
     4561 tttgtccggt tacttccata ttcctgagga aatcgatccc cgaatacact ccttcggcat
     4621 cttctccttc agtaccgagc aaggtgcctt tctgcgaccc aatggttaac acaaaactat
     4681 cgtattcatc ccgaatttcg gcaaagctca ggttttctcc cagtttttta ttgcagaaaa
     4741 tacgcacccc aagttcagtg attgtagtaa tttcctgatc gagaatatcg ttgggtagtc
     4801 tgtattccgg aataccatag cgtaaccagc cacctggttt gggcattgct tcgtaaatat
     4861 cgcattgatg acctttttgc tgcagccagt atgccgctga caatccaccc ggtcctgctc
     4921 cgataattgc aattttttta ccggttgaag gcgcaacggg agctttatag tggttgtcgg
     4981 aattcaggtc tgcatctgct gcaaaacgtt tcagataatc aacccccaca cctgctcctt
     5041 catctaaaaa attcctccgg caattaaatt cacagggcct tacacaaacc cttccacata
     5101 ttgcaggcaa aggatttttc tcttttatca gtttgatggc ttcggaatat tgccttttct
     5161 caatcaggct aatataaccc tgaacatcaa catgtgccgg gcaggctatt ttgcatggac
     5221 ctacgcaatc ggcatagtgg ttgctcataa ttaaatcgat tgccgttttg cgtgcaatct
     5281 taactcgttg gttttcggtt gatatcttca taccatccat tacacgagta gagcaggaag
     5341 gctgaagatt cttcattcct tcaatttcga caacgcagac aaaacacgat gaataaggag
     5401 ccagtcgtgg atcgtgacat aaggtcggga ttttaatgcc aatcctgttg gcaagatcaa
     5461 gtatggtttc gccttttatt gcttcatatt cagtattatt gacaaagact tttatcatgg
     5521 gttcattttt tttaagttaa actgattgca ttaaaattac aatttgtgta gcatgagcca
     5581 cattgaatac acttatcctg atgaatgaaa tggggcttct ttttttcacc agtaattgct
     5641 ttggtggggc atttaactgc acaaattgta catcctgtgc atttggtttc atcaatctga
     5701 tacgtaatca attttttgca gacttttgca gggcattttt tcttgtaaat atgtgattca
     5761 tattcatcgc ggaaatagcg aatggttgac aacactgggt tcggggctgt ttgacccaac
     5821 gcacacagag agttttcgat aattcgtttg ccgaggtctt ccagtttttc tatatctcca
     5881 tcttcaccct taccatcgca aatgcgttca agtatttcga gcattcgttt tgttccgatt
     5941 ctgcaaaaag tacatttacc acacgactct ttaacagtga agtccaggaa aaatcgcgcg
     6001 atatcaacca tacaagtagt ttcgtccata accaccattc cccctgaacc cataatggct
     6061 ccggtggcct gcaacgaatc gtaatcaata agagtgtcgc caagatgcgc tggtatacag
     6121 ccaccagagg ggcctcccag ctgaacggct ttaaactttt tgtcgccgat gattcctcca
     6181 cctagtttgt aaataatttc gtttacggtg attcccatag gaacttcaac gagaccacca
     6241 tttttaattt tccctgtcaa cgcaaacacc ttggtccctt ttgacttttc ggtaccatag
     6301 gcagcatatt tttccggacc attattgata atccacggaa catttgccca ggtctccaca
     6361 ttgtttatat tggttggttt cgaccacaaa cctgattcgg ccgggaacgg tggtctttta
     6421 ttaggcattc ctcttttacc ttcgactgaa gcaatcaatg cagtttcttc gccacataca
     6481 aaagcgcctg caccttcttt cacatggatg tcgaaattaa aaccctggat gccgaatgca
     6541 tttttaccca gataaccctt ttcccttgct tgttcaagag caatattgag gcgtataatg
     6601 gcaagcgggt actctgccct gcaatagata actccatcgg tagcaccaat ggaataggcg
     6661 ctaataatca taccttcgag taccgcatgt gggtcaccct caagcaggga tctgtccata
     6721 aatgcccccg gatcgccctc atcagcatta cagataacat atttttcatc gccggaatat
     6781 gcttttgcaa actgccactt ttttcctgtc ggaaagccgg cgcctcctct accccgtaaa
     6841 cctgaatcga gaaccatctg aattacagag tctcctgtaa ttttttcaga ggcaattctt
     6901 ttcatagctg tatatgcacc gtttttctcg gcttcttcaa tgttttcagg gtcgatatat
     6961 ccgcaattcc tcagggcaat tttaacctga ccatcgaaat aggctttacc cggcaaatca
     7021 agtttatcgt taaacacaac ataactaagc aaaggctgat tatgtttggt atgatattct
     7081 aaaatttcaa cgatgcgctt ttcatcgaca gcaccataca accatgtttc atgaccatta
     7141 ttaatttcga caagcggttc attaaaacac attccaatac agctggtttt agcaaggtcg
     7201 aaattataaa tttcatcttt ctgcaattcc tgaagtttat tatatgtttt tccggcaccg
     7261 gcagcgatac cacaactgcc caagcctact gttactttaa ctttttccat tctgttcaat
     7321 ttgaattagt ttttgctttc ttcgatatct ctctgattac tttaactgcc gatttaccat
     7381 cgagtttgcc ataagtattg ttgttaacca tcattaccgg tgctaatgag caacagccta
     7441 aacaagcaac tgattcaagt gtaaacaaac catccggggt tgtttcacca tctttaatat
     7501 gcaattcctc ggccaatgct tcacctacgg cattcgcatt ttgtacatgg catgcggttc
     7561 catgacacac tttaataata ttttttccta ccggattcag tctgaattgg gtgtagaaag
     7621 tagcaacacc gtacatatcg ctaacttcga gtccggtctc ttttgcaatt ttatcgaagg
     7681 cggctctggg aagataccca aaaatagcct gagtggattg aaggatagga ataatacttc
     7741 ctttcttgtt tttatatttc ccgattactt catccaattc atttgtaatc acaagcgcat
     7801 caagctgctt gtcttgacta tggtcagcat aaattctctt tattctcata atacagggct
     7861 taaaattttc cagtaaaatt aaaaaaaggg gcttaaaaaa gcccccctaa acaaacaaaa
     7921 aagcaactaa acaactccgt gagcgatcat tgcatcggcg accttaacaa agcctccaat
     7981 gttggcgcca tcaacgtagt taacaaagcc atcagctttg gtaccatatt ttacgcaagt
     8041 agcatgtata tccttcataa tctggtgcag acgggcatca acttcttcgc gtgtccacga
     8101 caatcgcatc gagttttgac tcatttcaag tcctgaagtt gaaacaccac cggcattggc
     8161 agcttttccg gggccaaaga gaatcttatt cttgagatat atttcgattg catcgggtgt
     8221 tgatggcatg ttagcacctt ccgaaacaca gatacaaccg ttattgacga gagtttcagc
     8281 ttctgcacca tttatttcgt tttgtgttgc gcaaggcaat gctacatcgc atttcttaat
     8341 ccaggggcgt tgatttgcat ggtattcgca gccgtatttt tcagcatatt ctttaatacg
     8401 tccacgtttt acgttcttga gatgcattac ataagcaagt ttttcgtcat ctataccttt
     8461 ggggtcgtaa atgaaacctt cggagtcgga aagggtaact acttttccgc ccagttcatt
     8521 cactttttct acggcatatt gagccacatt acctgaacca gaaacagcaa ccattttgcc
     8581 ctcgaagctt aaaccacggg tttttagcat ttcctgagca aaataaacac aaccatatcc
     8641 ggtggcttca ggacgtatta gcgaaccgcc ccattccagt ccctttccgg taagaactcc
     8701 ggtaaattca ttacgcagct ttttatacat gccgaacata taacctattt cgcgtccacc
     8761 aacaccaata tcgcctgctg gcacatcggt gtcaggtcca atatgacgga acaattccat
     8821 cattaggctt tggcagaaac gcatcacttc tgcatcggat ttgcctttcg ggtcaaaatc
     8881 agatccccct ttgccaccac ccatgggtaa tgtggtgaga gaattcttaa atacctgttc
     8941 gaaagcaagg aatttcagca caccgagagt aaccgtcggg tgaaagcgca taccaccttt
     9001 atagggtccg atagcactat tcatttcaat gcggtaaccc ctgttaactt tgatttcgcc
     9061 tttgtcatcc atccacggaa tcctgaatat tataattcgt tcaggctcga ccattctttc
     9121 aaggattttc gcatttttat acttaggatt ttcctcaaca tagggagtta acgactctac
     9181 tacctctcga acagcctgat ggaattcagg ttctgctggg tttttggcaa ttacacctgc
     9241 catgaaatca tcgacgattt gctgatactt gctcattgtt ttaaatttta aaaagtttga
     9301 ccaaaaatta attctggtat aaatgtaaat agtaaatttt tacatttcaa agcgcagtta
     9361 agcactgaaa ataaatattt acctctaacg cactcataat caatacctaa agcctattta
     9421 tgttttgcag catttttaca tattttgacc cataacatga ttattatcat ttttttagac
     9481 tagtatgact tttgtcatgt ttttaaacag tggtatataa cattgttttt gttatactga
     9541 ttaattataa tatagtttag ctgttattgt tttgaatatc aactttttat aataactatt
     9601 tatacattaa tttttcattt ttccttatta tcctcagttt tttggattac atagggtttt
     9661 gttctatgtt tgttaaaaat gtgaatcatg aagcttagta ttgccttttt attggtttta
     9721 tgctccagtt atttgtatgc caccacatat tatgtaacca cgactgctga tgcagggaac
     9781 gggtcacttc gtcaggcaat tacctctgca aacggaggtc ccaataatac aatagctttc
     9841 aatatccctt caacagacac aggctttgat gagcaaactg gtgtgttcac tattcatgtc
     9901 agcaccctgc tacctgcaat tcaaatgatt aacatgacca tcgatgccac gactcaaccg
     9961 ggaaacacca atatttacgg tccggaaatt tgtttaatgg gaaacgaatc gctgaattac
    10021 ggattgctta ttcctactta tggaaacgta attaaaggtt ttatcatcaa tgggttcaaa
    10081 tgtggggtac tgatttataa aaccgcatat ctaagctgca ataacaatac agtcagcgac
    10141 tgctacctgg gtgtaaatta tgatggtagc atgtctgccc caaacgaaat aggtatagca
    10201 atttacgatg acgcaaccgg caacaccatt cgggataatt tgatttcagg aaatacattg
    10261 gctggaatag gtatacaatc atcgaatact aattatatct atggtaaccg aatcggttgc
    10321 gacagaaatg ttagtttcgc catacccaat tactatggag tagctatcga ctcatcgtca
    10381 aataatatag ttggtggcac caccatgcag catcgaaact atatcagcgg caatacctat
    10441 gccggcgttg ccatcaactc ggttttatcg cacaacaata ttgtaaaagg taattttatc
    10501 ggtgtaaatt cgtctgcaat tgccccctcc gataccctat ccaattatta cggtgtagcc
    10561 attaacgaat cgtctaataa tattattgga ggaagtacta cgtctgaacg aaatattatt
    10621 tccggcaatc ttgaagccgg tgtggctatt cttggtacat cagccacgga taatattatt
    10681 ataggtaatt atatcgggcc caatgcagat ggcaccgatt caattgccaa tggaaatgga
    10741 atccttctca atggggccaa tggtacaata attggcaata cttcggctgg agggggcaat
    10801 gtgatttccg gaaatcatct ctccggtatt tcgatggctt atagtggcac agaaaacaac
    10861 attgttaaag ggaacctgat aggaactgac aagaccggaa tgaatgcatt gagcaatcac
    10921 accggcatct atttattcag caatgccaac aacaatatta ttggtggcac aggggaaggt
    10981 gaaagaaaca ttatctcggg taattatgaa cttggtattt cgatggaggc agtttcggga
    11041 aacattgttg ccggaaattt tatcggtcct gatataagcg gaaccggaac gttttttatg
    11101 tctggcgata tatctattca aggtaatggc ttatatttca attcaaattc aaccggaaac
    11161 agtgctgtgg ggaacctgat ttccgggaac agggtgtatg gactgattta ttatggtaat
    11221 tcttctgata atagttgcga aaacaatttt attggtgttg atattactgg gaacactgcc
    11281 ttaccgaatg caacgggtat ttgcgttgac ggagcagcca atcacaatcc tattacggga
    11341 aatgtacttt caggcaatct cgcttatgga atttttattg taaccacagg gacctattat
    11401 aacgaactga aaggcaataa aatcggcacc aacgccgatg gtacggcagc cgtacccaat
    11461 caaataggtc ttattatcgg aggaggtgca aaacaaaata taattggcgg ctcattgcca
    11521 gaacatagaa acattatttc gggaaatact ttcgaaggga ttgaagttgc tgatattact
    11581 accatgtaca acactatatc cggaaattat atcggcaccg atattacggg cagtcaggct
    11641 atacccaatt ttaacgggat tggctttgca acccgccctt ctcataatac aatcaataac
    11701 aatgttatca gcggaaatga aaacatcggt gtgatactgt atgaattttc cgactcaaat
    11761 acggttgtct ctaattatat aggtactatg gctgatggcg taaccgccct agggaacgga
    11821 tttgcaggag ttgtgattga aaattcgaat cataattcta ttgggcaggt agcaatggga
    11881 aataccattg ctttcaatgg cactgcaggg cttgttgttg cggacgaaaa ctcatttgga
    11941 aacacttttt cggccaatct gatttacagc aacaattcca tggaaataga ccttttccct
    12001 gcaggtgttg caacaaacga cgcgggtgat agcgactcgg gggcaaacat gcagatgaac
    12061 tatccggaga ttactcaggc agcttatgat ccggtagaag gcatcgcttt gattgaagga
    12121 accatcgata atacagtgaa cggaagtgca ggcggcatcc gcatcgaagt tttccgcagc
    12181 aacgaaagca atccttttca gcatggcgga gctgatttgt atctgggttc gggctatgca
    12241 aacgcttctg gggaatggca tgtgtgggca ccaaacctaa ccgatagcga taaaataacg
    12301 gcaacagcca ccgataccga aggtaacacc tcagagttca gtttgaattc agatgtattt
    12361 gtggcatctc ccgaaattct ttatcgtaca aacgaattta ccatatttcc aaacccggta
    12421 aacgagaatg tttggatcag ctccgataaa tggggaaatt acccatatcg atttacatta
    12481 actacaagcg acggaagatt ggtatggacg aaggaatgcc tgcgcattga caaaagcatt
    12541 atgcttgaac tcggagagaa cgttattcct tccggcatgt attttttaag catatataaa
    12601 gatggaatca ttaccactac aaaaaaaatt gctgtaatca aatgaaattt tattgtgcct
    12661 atataaaaac gttagtatta tttatcgtac tactaattgg taatcaggca attgcaaata
    12721 catttattgt taccaatact gccgattctg gtaatgggtc gctacgatgg gcaatacaaa
    12781 actgcaacat ataccccggt agtcatacaa ttctgtttaa cattcccgag tcggatggca
    12841 attacaatga agcacagggc gtgtggcgga ttgatttaac ttcgaacctg cctatgctga
    12901 atcacagcaa tgttctaatt gatggacaca gccaggctac ctatagcgga gataaaaacc
    12961 cctatggacc agaaatagtt ctggatggcg gaaataacga ctgggccgat tacggtatta
    13021 acatttacaa tgcttcaggg attaccattc agggtttaat tattgggggt tttgtggtgg
    13081 gtattcaaat gactggagaa aatgctttta acaatacgat ttcgggcaac tatatcggtt
    13141 gtaattacaa tgctacagaa tcgaacggga atacacatgg aatttatatt ttaagtggtc
    13201 cgcacaataa tatcattggc ggaaacaccc ctgtcgcacg taacatatta tcaggcaaca
    13261 accacacagg catgcgggta gtgaatgcaa gctacaatac cattcaggga aactatgtgg
    13321 gattgaatcg gaccggaact gcagcactgc acaactacga tggtataagc attgaaggaa
    13381 tatcgcaata taatctgatc ggtggatata ctccagaaga aagaaattat gttagcggga
    13441 atatgaacta cggaattcct gcattcggga ttggctgcta ctacaatatt atagccggaa
    13501 actacgtcgg tacggatatt acagggaaca atgccattcc caatacttac ggagttcttt
    13561 tcgacgacgg agcatgttac aatacccttg gaggcataac cgagggtgca ggtaacctgc
    13621 tttcgggaaa ttcaggttat ggcgttttca tttataactt tggaacaatg aaagatactg
    13681 tcagagggaa cctgataggc acaaacgctg aaggaacagc agctgttccc aatgcaaacg
    13741 gcattgtgat agatggtccc tcctaccaac ataccatcga taataatgta atttcaggga
    13801 atttgcagat gggaattgat attcacattg caggaagcga tagtaatatt gtaataaaca
    13861 ataaaatagg cacagatatt tcagggatga atccgcttgg aaatttaatt gatggtatca
    13921 ggattgccga aggtcccgat aacaacatta ttggtagacc cggacaggga aatattattg
    13981 cttataatgg cggaaacgga gtaacaatta tgaccgctgc tgagcaagcg aacacaattt
    14041 cgggaaacag tatctatgcc aacgaaggtt tagggattga ccttttccct gagggcatta
    14101 ctgcaaacga cgaaggagac tccgattccg gtccaaattc taacatgaac tttccggtta
    14161 ttacacaaat ttctattagc acaaatgttc agctttgggg gcatgtcgac agtcctggtg
    14221 ctgacgaaat tacagttgaa ttttttaaaa gcgattacta cccttctggt acaggacaag
    14281 gccgggaata tataggcagt tgcagtactg atgcatcggg aaatttcagt tactcaattg
    14341 ctggaggatt aagtggtgcc atttatatat gcaccacagc aaccgatgga tcaggaaaca
    14401 cctccgagtt ttcggcaaac aaattatgtg tacaaacagg tattgatcaa catgttgaag
    14461 ttacagaacc agtgagcatt tatccgaatc ctgcagcaac gagctttcgg tttaataatt
    14521 ttgaaattgg acgtcggttt ttaattgtaa acaattctgg tcaggtaatg ggttcgggta
    14581 tctggaaagg agacaatatc ccagtgagct atttactgcc agggagttat tatgtgttgc
    14641 ccaccgatgt tgcagcacat gttatacttc taattattat gcgttaatac tttattggtt
    14701 gctagagctg aaattcactt ttttgttttt cggtattaag ctaataactg ttaataattt
    14761 tccataaaag catatgcttt actaattgat atttaagaaa tttgagcaaa tattccaaag
    14821 aaatgaaaat acatctgata gcatgtggtg gggctgtaat gcataatctg gccatagcgt
    14881 tgcataaaaa agggtatacg gtaacaggtt ccgatgatga aatattcgaa ccggcccgaa
    14941 gtaatttagc taagtacaga cttcttccgg aagcatgggg ttggtttccc gataaaataa
    15001 cgtccgatct acatgcggta attcttggta tgcacgcacg gcgtgataat ccggagttgg
    15061 taaaagccct gcagtcgggg gtaaaagtgt actcgtttcc ggaataccta tatgagcaaa
    15121 ccaaaaacaa aacgcgtatt gtggttggcg gaagccatgg taagaccagt gttacctcaa
    15181 tgattatgca tgttctcaac cggtgcggaa tgcacttcga ttatatggta ggtgctaaaa
    15241 tagatggttt cgacactatg gtaaatattg aagaagccaa cgaaattgct atttttgaag
    15301 gagatgaata cctcacttca tgtatcgacc ctcgccctaa attccattgg tacaaaccgc
    15361 acatagcggt gcttactggt attgcatggg atcatataaa tgtttttccc acattcgatg
    15421 actacattcg ccagttcagc atgtttgtgg atatcatcga cgaaacaggc tgtctcattt
    15481 attttaaaaa cgacaaaaac cttcagacgc tagtagaaga aaaaacacgg ttgcgttgta
    15541 tgccctatta tgaacttgat tctgaaatct cggataacaa aacaataata aaactccgaa
    15601 ataacactta cgaaacaaag ttgttcggca aacacaatat gcaaaacatc aatgcagcac
    15661 gactcgtttg caacgaaatt ggcattaaca atgaacaatt cttcgctgca ttatccgatt
    15721 ttaaaggggc cgcaaaacgg ctgcagctgg ttgcagaaaa taaatcaagt gcattttata
    15781 tcgattttgc ccatgcacca tcaaaactta aagcaacaac cgaagctgtg aaacaacgat
    15841 acagcggaag gaaactggtt gcctgcatcg aattgcacac ttttagcagc ctgagcaaat
    15901 cgtttctccc acaatacaat ggcagtatgg aaatggctga tactgctatt gtgtattata
    15961 atactcacgt tctcgagcat aaaaacctgg agaatatcga ccccgaaatg gtgaaacaag
    16021 ccttcggaaa tgatgtgctc gtatttaccg attccctact cttacagaag ttcttacttc
    16081 aaactgactg ggataatacc aatttgctga tgatgagctc cggcaatttt gatggtatta
    16141 attttgatat gtttgctaaa caaataattc gttgattaat tgacatttat gcttctaaaa
    16201 atcattacat tactgaacca tcatttcccc tacaaggcaa tacttgcaat catactggta
    16261 ggaatgctca ccggagcaca atcatgtaaa tatcaggaaa tcacaattgg aacccccaac
    16321 ggtttaagag taaaagatat cagcctcgaa aaagtaaatc tgcaaattga tattccaatt
    16381 aataacccta ataatttctc atttaatatc agtaaaaccg aattggaagt ttacattgcc
    16441 ggagttaaaa taggtacaat aaccaaattc gaaaaaacca aaatcgatgc ccgctccaac
    16501 gaagtttacc cggtaggctt ttcattcaag ccaaccgata tattggggaa ttcaattaag
    16561 cttgtttcgg aactgagtaa gcgaaatacc gaagttgaag ttaaaggcaa aattagagta
    16621 agtaaattca taatcgcaaa aaacataaaa gttcattcaa aagagacggt aaaaatttat
    16681 taatttgtaa aatattctat tgtatatgag aacattaatt ataggtctga ttacatttct
    16741 gttcagcggc accctgtttt cacagtctgt tactccggtc gacattccgt acttcaacgt
    16801 aggagcttat ggcggtaaaa ttcaaataca tcagtccgac aaaattgaga acttctacga
    16861 aacttatgtg ggtattaata aaaagcgtaa cgggttttat ggttaccgtg ttaaaatttt
    16921 tgctgatgtt ggtcctactg cccgaaaaga agccaacgat atcaaggtgc gcttcaactc
    16981 ctcttacccg gaatataagg catacctaat atacaatgaa ccaaattttg aagtacactg
    17041 cgggaacttc agaacccgta tcgaagcgat ggcattgctt agtaaaatta aaaatgctta
    17101 cccggggtca ttcatcattt atgaaataat tgagtttccg aatttgtaat atttgcaatg
    17161 cccgacattt cgaatctcat acaaaaagct gcagaaatac gccgcgatgt tattaaaagt
    17221 ctgaacaaag ccggctccgg tcatttgggg ggggcattag ggttggctga tgtatttact
    17281 gtattatact tttatgaact ccgacataat tcgggaaatg ctgagtggca tgcccgcgac
    17341 agagttgttt tatcaattgg ccatgttgca cctgttttat acactactct ggctcatgcg
    17401 ggttatttcc acgaaagcga actgggcacc ctgcgtaagg aaggctccag actacagggt
    17461 catccatcat tgcaacataa gcttcccggt gtggaaactt ccagcgggtc tttgggtcag
    17521 ggcttatcaa ttgcagtagg aatggcattg gcaatgaaaa ttgaaaaatc gatccgtaat
    17581 atatactgca tcctgggaga tggtgaattg caggaaggcc agatatggga ggctgcaatg
    17641 agtgcctcac accataaact cgacaatctg cttgcaatag tcgaccgtaa cgaagtacag
    17701 attgatgggt cgacacgtgc ggtcatggat ttggagcctc ttactcaaaa atgggaatcg
    17761 tttggctgga atgttatcgt ttgtagaggg aatgacattt ctgaccttat cagaaccttc
    17821 gaccagatta gaaacatcag gaataagcca tctgtaataa ttgcccgtac cactatggga
    17881 aaaggtatta gaagcatcga aggagattac acatggcatg gcaaagtacc tgacaatgag
    17941 caatgcagcg catttttaaa agaactggga ttttaaccca gatacatcag aacagtactg
    18001 tagcttcagg ttattacgaa aaagattcaa aaatacttgt ttaacagctt taatgttttt
    18061 gataatgatg taattatttt ttttattaca tttggagaga atacaaaaac acgcattatg
    18121 aaagctgact atatttgtcc aaagtgccat gggcatttaa aagttaaaac cagcatcatt
    18181 cttcttacaa aaaacagtaa gggagactct gcgctggtat tactaagccc ggaaatcggt
    18241 gattacagtt tcaccattca cccatcagtt aagattgccg aaggcgaaca taccaatttc
    18301 atttgcccaa tctgttacga aaacctcaat actgatgatc ttgaccgtaa tttggctaaa
    18361 ttgatcagaa ttgatgaaaa caaccatcga tcagaggttg ttttttcgca gattaaaggc
    18421 gaaaaatgta cttttaaaat aaatgaagga ggcattgagg ccttcggtga cgacacaaga
    18481 aactatttga atcatttcgg tcttcaatcg gaataatact actccagata gattcttttt
    18541 ggctggcgag ctaacacttt gccaattacg gcagtttttc cgaagcgtgg atccgtttga
    18601 aaatctaata tcatttgttt tgtattcttt gccggacaac taataagcag cccgcccgat
    18661 gtttgggcat cgtgaataat aacattataa aaatagtcaa accgccaggg aataaatatt
    18721 tcattttctg ttgatttcat atttcggaaa actgcacctg gaatacatcc ccgctcaaca
    18781 aattggttaa tacctgtaaa atatggcagt gattcagtat aaattttcat gctgacattg
    18841 ctagcgattg ccattttcaa agcatgacct gccagtccga aaccggtaac atcggtagca
    18901 gcccgaatgt ggtatttctg cattatctcc gaccctgttt tatttaacaa acacatgctt
    18961 ctgatggctt catcaagtac ggttgtttca gcaataccgg ttttatgtgc tgccaaagca
    19021 atgccggtac ctaatggctt tgtgagaatc aggtcatcac cgggctctgc tgctgcgttg
    19081 gttataatat ttccagggtg aacaaggcca acaacggcca acccatactt tggaatacta
    19141 tcggaaattg tatgcccgcc tgttataatg caatcggctg cggcagcagc atcgttacca
    19201 cctttcagga tttcatgcaa ctctgtgagt tcaataccga cagccggaaa catcacaagg
    19261 tttagcgcca taagcggctt gcctcccatc gcataaatgt cgctgagtgc gttcactgca
    19321 gctattttgc caaatgtgta ggcatcggaa cacacaggcg ggaaaaaatc ggttgtaaaa
    19381 ataagtgctg tttcggaatt gaggcgaaat atagcagcat catcaccggt agaagcacca
    19441 accaataaat cccgatgtac cgccattggt atttgtgcca atatcccctc aaggtcttta
    19501 gctgagatct ttgcagagca accacctgat tcgcagaatg acagtaaatc cgttttcata
    19561 ctacatttcc agtttttcac attttaagcc atgttccgat aaaaattcat acagttgtat
    19621 ggcagtcctg acgtcagcgt taatacacag tccgcattcc gctgaaatat gttttggaac
    19681 gggtacaata gtaaactgaa aaaattgtga cttcgcaagc ttttcagcca gtattacatc
    19741 gcgggtactc cggaaaagaa tgtaatattt ttcatgcatg agcggcaatt tttttcaacg
    19801 cagtgtaaag gtagtcaaaa tcgtgtcgtg tatgataggg cgatatagaa attcggcaag
    19861 taccctgtgg gaatgtttgt aatgtagtgt gggcaagcgg agcgcaaagc aaccctgatc
    19921 gggtttcgat acctgattgc tgaaacagcc gttctgataa ttcggagctg cttaacttgc
    19981 tgtgcacaag tgaaaaaaca gcagaatggc tgccagggtg atttgatgta taaagcctat
    20041 agccctgcag ctctgatatt ttactaatca ggtcttccaa ttcaattatg gaatggttag
    20101 gcgtgggctt atgatttatt gccgcaagca aaccggcaat gccaactata ttatgcgttc
    20161 cagcctcaaa tttatcaggt aaaaaatcag gcatttgaaa actcgccgag ttcgacccgg
    20221 ttcccccatg aataaatgta ttcattgttg caggctctct tacaaaaaac ccacccgttc
    20281 cggttggccc cattaaggct ttatggcctg taaagcacaa ataatccact ttccagtcgt
    20341 cgagaaatac gtcgtagctt ccgagcgatt gtgaagcatc aacaagcatc ggaatattac
    20401 cggagatacg ttttatcgct gcgatatcct gaaccactcc atttacatta cttgcatggt
    20461 taatgcatac cagacctata tcggcagaaa gcatggtttg taaagcctga atgtcgatga
    20521 ctccattttt cagagcagga attatcccga aatcagtaat aagcgcatta tctttcatgt
    20581 attgcaaagg acgcacaaca gcattgtgtt ccatcggaga aacaagcacc tttttacctt
    20641 tcaaaggtaa agtttttaaa actgtattta ttgcatgcgt tgcattaagg gtaaaaacaa
    20701 tattctctct caactctgta tgcattattg cagcaagcgc atccctgcat tcttcaacaa
    20761 tacccgttga cattagcaca cgcttatagg caccacgacc atagcttgtt cccagctcat
    20821 ggagaaatga agtcatagct tgggcaacct ctgggggctt gggccaagat gtggcactgt
    20881 tatcaaaata aacattcatc tcaaggtctg attattgatg tagctttcga tagcacctca
    20941 acaatggtgt acatattaga aacctgtcct ataccaattt tatcacgtat ttgaaagtag
    21001 tcggcacagg taccgcaaat atgtatataa acacctttat tttcaagtgc tttcagcgac
    21061 cccagcacat cactttcgca atgggtaagt aaaacaccgg agttatagaa cacaaggtgg
    21121 gaaggaaggt tctccgcttc atttaatgta tttataaaag ctttcatcag tattctaccc
    21181 aggtcttccg gtccgctacc catagtttcg gaggtaactg caaccacatg tgatcggtat
    21241 gagtttattg agcaggatgt ttcggagcta tttgtttgag ttgctgattt ttttgccgtg
    21301 acaaaaaaag tatcgtcctt tttagcgcaa acaggctggg cttttaaatc ccgcagaaat
    21361 gtcatcaggt tttgcatact ggtttcacta tcggttgaga tacaaatact ttctccaacc
    21421 gcagctttgt cgagtgcctg ctttgcaagt attagcggct gagggcacgc aagcccgcga
    21481 gcattaatgg taatcataat cctaatatgt tggctttatt aatcaactgg tttgcaactt
    21541 cggaagcgct taatctttgc acatcaattc tatgaattgt atcagcaggg tgtttaccca
    21601 gtgaatgcag gtaagcttta tcgtaataca ctagggttaa ttctgcaaaa agttttaaat
    21661 cgccctcgac caaagccttg gttgcttttt taaaattatc gcctcccagt ttttccgaaa
    21721 ttttaccaag agcttcatgt aacgcggaat tatcagcttc ggaataatcg cgtaccaatc
    21781 tgtcgagtct gaattcttta tcggattcca caaaaaacag gggactttga aacatttttt
    21841 gccataattc gtcaggaata aaattttttc caatgctttt gctttcgtct tcaatccaaa
    21901 ttctcttttt tttatcgaag cattttatca cttctgcaag gttgttttcg aactgttcgt
    21961 tagtactctg aggcttcatt ccaatatgtc cgaatgccga tcccttatgc gatgcaatgc
    22021 cttcgaggtc gacaacctgc tctcctcttt gttttaattc aaataatatt tccgtttttc
    22081 cggtcccggt tttacctcct acaatacaaa gtttatttac ttgggcaaac aactgcctta
    22141 tgtaattacg atatgcttta taacctcccg acaggcgttt cacttcaaat ccggctgaat
    22201 gcagtaacca tgtcatgctt tcacttctca ttccaccccg ccagcagtag attattgcct
    22261 tttttcccgg aagaagtttt tgtgcagttt tgagcatatt tgacatttcc ggtccaagta
    22321 actgataggc tttaagaaca gcagagaact tacccgattg cttataaatg gttccgataa
    22381 ttactcgttc ttcgttactc atcagcggaa agttcactgc tccggggatg tgccctgctt
    22441 caaattccaa aggtgtgcgc acatccagca caatcatttg ttcagccagc agcaggaatt
    22501 cgtttatgtc gatttcattt tgcattacgc aaaactactt caaacaaacg aaatatgtaa
    22561 tgtttttttt tactattccc aattcccatc aatcgaagta agaaaatatg gagctatttc
    22621 accatttcag catagtactt atagaacaac ggaatagtct cgattccctt aaaaaagtgc
    22681 tccagcggaa agttttcatt aggagaatga atagcatctg actcaagtcc aaaaccaagc
    22741 agtatcgatt ttataccaag cacatgctcg aaagttgaaa taatgggaat gcttccacca
    22801 ctccgaaagg gtacggcctg tttaccaaaa gtatcgaata gagctttctc tgcggctttg
    22861 tatgcggggt ggtcaaccgg gcatgcgtaa ccctgtcctc cgtgcaaagg agtaactttc
    22921 acacttacat aatcgggagc aatagacttg aaatgttttg caaataattc tgatatttta
    22981 tggtgatcct gattgggaac cagccgtgtt gatacttttg catacgcttt tgagggtaat
    23041 actgtcttgg ctccttcgcc ggtgtatcca ccccatattc cgcataaatc gaaagaaggt
    23101 cgaattcctg ttcgttcagt agtagtgtat cctttttcgc ctttcaacgc ttttactttc
    23161 agtgcttttt tatattcctc ttcgtcgtaa ggtgccaatg catacaagtc gcgttctttg
    23221 cgagagacct ccagcacatc gtcgtagaat ccaggaattg taacctttcc gttttcatcc
    23281 tgcatttggg caatcatatt gcagagcaca ttaaccggat ttgctacagc gccgccaaac
    23341 aaaccggaat gtaaatcgcg acagggcccg gtaacctcaa cctcccaata ggagagccct
    23401 ctgagtccag tagtaatagt aggtattcca ggaccaatca taccggtatc ggaaaccaaa
    23461 atcacatcgg ctcccagcat ttctttattc tctgcacacc attttgtaag cgacggggag
    23521 cctatttctt cttctccctc aatcatgaat ttaacattac aatgtagtgt acctgttttc
    23581 accataatct caaaagcttt ggcatgcata aagccttgac ccttatcgtc gtcggcaccg
    23641 cgagcccaaa tcttgccatc tttaattacc ggctcaaaag gttttgtttc ccacttatca
    23701 accgggtcaa ctggcataac gtcgtaatgt gcatagacca aaatagtagg cagctttgga
    23761 ctcacatact tctcaccaaa cacaacggga ttgccatctg tgttgtatag gtcggctttg
    23821 tcggctccgg cttgaagtaa catttgcttc caatattccg cagctcttat catatcgttt
    23881 ttatgctccg acaatgaact gatcgacgga attcggataa gaccaaacaa ttcgcttaaa
    23941 aacctttctt tgttggcttc gatgtaattt tttgttgtac tcatatcaat gattattaat
    24001 tatttacgcg gaggtttaaa ggcagaacta aaataaaaat gaagttgcac accaaccacc
    24061 tgatattgct tattaaagct gccatacagc gatgttccaa tacccagatc gtagaagaat
    24121 ttccaggtaa actgaagctc agtgtgtgca cctaaagtat gaaaataggt gtaatataca
    24181 gttccatacg gatcggtata attatataag gagccaaacg accatgtggg tcctataaaa
    24241 aaagcaaaat taaaatgtat cttttcaatg cgtaaaccgg cacctaagtg cacatcgttt
    24301 aaccgcacca taggtctttt tagaaaaaac tgatcgctgc tgtaatgata tcccagatta
    24361 aaatacatag catgatagcg gtgatggtat cccagagcaa aattggtttg ctgttttctg
    24421 agattggtag catacccata tccaacgcct gctgtgaacc agttactgcc tgttttaaaa
    24481 accttgtcgc caactttaat ctgcgggaac atcttatccc atttcgaaag cccatctgtt
    24541 ggccgggtat cgcgtggctg aacctgagca ttcaataaaa caggcagtgc accaacagca
    24601 attagaagaa tataatattt caagagccat ggcatacttt tcaatttttc ataaaaataa
    24661 taaaaacgtc gagaacgaaa atttatttct ttaaggatac tgctgttttt atgttttcgg
    24721 ctgcataaac caattggttg taccattctt ctccatattt gcggatgaag gcatctttta
    24781 aaaaaacata tagcggaatg cctttatttt cacccgtttc tatggctgat ttgcatatat
    24841 cccaggtatg ataattcact gcatcgtagt atttataccg cgtaatcctt accgggtaca
    24901 aatggcagga caagggtttc ctgaattggg tttttccctg agtccatacc aactccaatg
    24961 cacatattgt aataccctgg ctgtttcgta tggcaaaaac acaggctcct tttccatgaa
    25021 gggtatttgt taccagatca ccttcagtat cgattacagc cacccccgag ttttgtatgc
    25081 ggttgcgcgc ttcagcagtg agtattgaag tcacttcggg caggcattgt tcaatttcgt
    25141 acttctcaga gtcttcgagc ggggctccgg aatcgccctg cacacaacaa atccctttgc
    25201 acaagcccgg attgcaagca aatcttttat cgatgatatc gaaacttaca agagtttttt
    25261 caatctctat catagcatta cttcatttgt actacagtaa ttccgtcacc gccaaagcgt
    25321 atgtcctcat cagaaaaata tgcaacttct ttcacgcttt tcagttgctc gcggatggct
    25381 ttccgaagca caccattgcc tttgccatgc aatatcttta gctgtgacac cccaagcaca
    25441 atggcagtgt cgacaaaaac agtgatttcg gataaggctt cttcggctct tttaccccgc
    25501 acatcgatag agggtttaaa tgtactgcgg agttcgttaa tattctgata taaagagcct
    25561 gcagaaatac gggttttagg actagtttcg ttatcgggaa ctttctcaag ctttgatttt
    25621 gcaaccgatg ttatcacact atcgaattgc actacggcat tctcgctgcc catgtcaacc
    25681 acttccccga ttgtttcgtg tcccgccaaa cgcaccttgc ttccaatatg tatttcttca
    25741 ggatcgatat caacaaactc aagctcttta tttgcatatt gcttaaactc ctcttcaact
    25801 gggctatcgg aatcaatttt taaaaattca tcattttttt ctgctacata tgattcaagt
    25861 tcctgcctta gtttgcgggt tttttcttta tcggcattaa tttcgcgaat gatgcggatg
    25921 gtactttcta tctgactgtt catgtccttg agaatctgcc ccgcttttat tttcgactct
    25981 tccagaattt tctttcgttc ggcgttaagt tttcttattt tttcttcgta ttcatgcatc
    26041 ttttgttcga gttgcttttc ttgctgtctg accaccaaac gtttgcgttc gtagtaccgc
    26101 ctgtccctaa gcgttttacg catcaatgac tctaacttag taagaccagt gcctgccttt
    26161 tcttttgcat cgattataat tgcttgagga aggcctatgc gatctgctat ctcaagtgcg
    26221 tacgagtttc caggaagccc tgcctgaaac tgaaacgtcg gcttcatcag gttgttgtca
    26281 ataagcatgg caccgttttc aatgccctct gtagccgagg caaatgcttt taggttggtg
    26341 tagtgtgttg taacaatacc gcaacatttt ttttcgagga gttgtttcag aattgcctcg
    26401 gctattgccc ctccctgcac tggctcagtt ccgctaccca attcatctat aaaaatcagg
    26461 gttctttcgc aggcgttttt tagaaagaat ttcatgttga gaaggtgaga actgtatgta
    26521 ctcaaatcgt tttctatcga ctgttcatca cccatctcga taaacatctt ctttgccatg
    26581 cccatacttg tgtttccacc acagggaatc agcattccgc actgtgtcat atattgcagc
    26641 agggcaacgg ttttcatagc cacacttttc ccaccggcat tcggccccga tatcagcata
    26701 atacgttgct tctcgttcag acttaaattc aatggtatca cccgacgatt ttcgttttta
    26761 aatgccatca gcaaaagggg gttttttgct tcgtgcagtt ctatattcat atcatcgtga
    26821 acctcaggtc gaatcgcatc catttctatt gccaatattg ctttcgccct gatgaagtca
    26881 agaatcacta acattccgaa caagccggaa atatcggact tataaggaat aatatcggct
    26941 gtaagctctt ttaaaatcct tcgtatctcc ctctgctctt ccagctcaag ttcccgctgc
    27001 cggttattca taccaactac ttctgcgggt tcgatataac ttgtttttcc acttgccgat
    27061 tcatcgtgaa tgagaccctg aatctttcgt ttatgcgtag tttcaattgg tagcaccgtc
    27121 cgaccattaa caatagttgc cgaaaggtcg ctgcttaacc agccttcttt gtgaaaggtt
    27181 tcgagaaggt tgttcaactt gctcgatatg agttttttat tggaaatcag cgagcttctg
    27241 acttcgaaca gcgttttcga agctttgtct tttatccggc catcgcgcga aaatacaaaa
    27301 tcgatacgat cgtaaacaaa ttgtggaaaa acaacacctt caacaaagct gtaaaggttt
    27361 cggcaggtat ctttattcgc ctttttaaaa taattaagaa cattttttga ctgactcaac
    27421 acattgaata ggtcaacgaa ctcttcctgg gctagtatta taccgtctac acacaattgt
    27481 ttaagcaaat ggcttatatc gggatattcc gtaatactca gttcactgtg aagcaacaaa
    27541 atggttttca tttcatcagt tgcagcaagt ttccgtttta tttcatctat atctgctaaa
    27601 accagtccgg taccacacaa ttcctttgca gttgttccct tgcagcgatg tgccaacatc
    27661 tcgcggattc tgcggaatcc aattttatgt tcaaaatcct cagggtagta caaagcggca
    27721 aattttttgt tatctttatg ctaaaataaa ctccagtatc atgcgcaaaa ttacattatt
    27781 attaataatt acaggtattg tcaacctgct ttattcgcaa aacgacacga ttgattcgca
    27841 gaatatggac attaactata cacgcgaagc cagttatccc ggaggtatga acaagctcat
    27901 aggagatata tggaattcta tggaattcac ccaagaagcc atagacaaaa agatagatga
    27961 aacgctgatg gtaagtttcg atgtaaaccg cgacagtaca atcagtggta tcatcctgct
    28021 taataatatt ggctatggtg ttgatgaaga atttatacgc gttctaggca aatacaaatt
    28081 tctaccagca ttggcacaag gaaacccaat aaagcaaaat gtaattataa gcattcctat
    28141 ccgcacaggt ccgaattcaa agaaaaaatc aaacaattag ctgagccctc ttgcctgatt
    28201 gatattgggg atgagtttct taaaattttg cagggcaatc aattataaac aggaaagcat
    28261 gtgttcagac tataaaagta ctgaatgctt agtattgacg gcaaacactt taaagtgtaa
    28321 tattcaaaaa aatcgcttaa ttttgcacat tcaacagtat cacataataa actgttgatt
    28381 cttgtgttta aagaatacta ttttaagtaa cgatgttgaa gcgtttaaca ataatattac
    28441 ttttcatctg tattattctg caggtttcag gccaacgaag gcatcccgaa aacctgccta
    28501 aactcgatca gcagctggcc cattttggtt attcacttgg cctgaactcc atgagtttta
    28561 caatacgacc atcagcggaa tattttttaa tggatacagt ttatgccatc gaaaccgggc
    28621 attttgtcgg gttcaacatc aacatgattg ccaacctgcg attgttggaa tatcttgatg
    28681 tacgttttct tcccgggtta attttcggtc aacgcaatct ggaatacaaa atactggaag
    28741 acagtatctt tcgcagacat accatgatga tagaatcgac gtttctcgat tttccgatcg
    28801 tatttaaata caaggctgcc aggctgaata atttcagacc ttttgttatt gccgggggtg
    28861 catttagagt tgacctggct gctcaaagaa aaatcgaccc cgaagaaatg cctaaaatta
    28921 aactcagtcc acttgataca tattatgaaa ttggactggg agcagacttt ttccttgaat
    28981 acttcatgtt tggcattgaa ttaaaagggt cgtttgggat tttcaatatg gtgaaatacg
    29041 acaatactga atatacgcgt gcttatagcc gattgaattc gaaaatgctg atactttcgt
    29101 tccattttga gggaggaaaa atcgacaaaa ttaaatggtg ggactaatac catgaaccca
    29161 agagtagttg aaatacagat gcaacttcca cctgaagaag catacaatat tggaacaatt
    29221 aaatcagcgg ttgctaaaga atcgggcatt gatatttccc gcatatcgca aatacgaatt
    29281 tcgaagcgat cgattgacgc acgccgtcag gtaaaagtac agtttactgt aagttacact
    29341 ctcgacggag agttgcttcc tgagcttgcc gggcagtggg agtttaaaga cgttcgcgtt
    29401 tcggacaagc aggtgattat tgccggttct ggcccagcag ggttgtttgc tgctcttcgg
    29461 ttgcttgaag caggtatttg tcctgtaatt atcgaacggg gcaaagctgt tgacgaccgg
    29521 aataaagata ttgcactgtt aaaccgtaat gaacactttg accctgaatc gaactatgct
    29581 ttcggtgaag gtggcgcagg cacgtattcc gatggtaagt tatatacccg aagcacaaaa
    29641 cggggcgatt cgaaccgtat attgcaattg ctacaccatt ttggcgcagg cgaaaccgtt
    29701 tttacagata cgcatgccca cctggggact gatcagctgc ctaaaataat aaaagctatc
    29761 aggcaagaaa ttgaaaatca tggcggtaga tttatctatt caacacggat tacggattta
    29821 gtcatcaaaa atggcaaagt atgcggaatt actacccacc ttggcgaaag catcgtttgc
    29881 gacgctttgc tctggagtac agggcactct gccagcgaca actacatgat gctgcagcag
    29941 cacggaattg ccatgcagga aaaaggtttt gcaatgggaa tccgcattga acacccgcag
    30001 gaactcataa acgaaattca atataaaaaa tacagtcatt ccaccttttt atcccctgcc
    30061 acttatttca tatcgcaaca agtggagaat cgcggtgtgt attctttctg catgtgtccg
    30121 ggtggtcaga ttgtgaatgc atccaccgcg accgacagca tggtcgtgaa tggtatgtcg
    30181 aacagcaaac gaaattcacg ttttgcaaat tcaggactgg tggtcgaaat cagacccgaa
    30241 gacgtacccg ggaacgacat ttttcggttg ttgcactatc agcaggagtt ggagcgctta
    30301 gcacgaacca atggaggccc acctatgaag gctccggcac aagctttggc agactttgca
    30361 gcaggaaata tttcgaaaaa tctacctcaa acgtcttatt tgccaggagt aatatcgtcg
    30421 ccggtacact tttggttacc tgccgcaata tcgctacgcc tgcaacaatc attacgctat
    30481 ttcggtaagt ttatgcatgg ctttgtcagc aacgaagcca ttgcgctggg ggttgaatcg
    30541 agaacgagtt ctccggttag aattctgcgt aataacgaaa ccatgcagtc cgtaagtctt
    30601 gaagcctttt tcccatgcgg cgaaggagcc ggctatgcag gaggtattgt atcgtcagct
    30661 gtggatggcg atatggctgc gcagaaaatt gctggatacc ttaactagaa aactatgaga
    30721 atcgatatca ttactgtatt gcctgaatta cttgaaagcc cgcttaatca ctccattatc
    30781 agcagggcgg ttaaaaaagg actcgccgaa atacatgttc acgacctgcg agagtatggt
    30841 attggcaagt acaggcaggt tgatgattat gtttacggtg gtgaagcagg tatggttctt
    30901 atgattgagc ctgtttttaa aatgattgaa aaactgaaat cggaccgcag ctacgatgca
    30961 attatttata cttcgcctga cggaaaacca tggaaacaga aaacagccaa tcatttttca
    31021 actttctcca atatccttat tctgtgcggg cactacaaag gcattgatca ccgcataaga
    31081 gaacacctga tcacccacga attttctatt ggtgattacg tgcttacagg cggagaattt
    31141 gctgctatcg tatttgtcga cagtatggtg cgcttgcttc cgggggccat ttcagatgag
    31201 cagtcggcac tgagcgattc gtttcaggac gatctgcttg caccacccgt atatacccgt
    31261 cctgctgaat tcaatggatg gaaagtaccc gacattttgc tttcgggtaa tcccaccgaa
    31321 attgaaaaat ggaaagacca acaggcactc gaacgcacac aacaactaag gcctgaccta
    31381 ttggaagact gagttgtttt ggttaatctc tacctttaat ttttcaacaa tctaatgcaa
    31441 cctttctgca taaatgttag ttattgatat gtaaaattta tacaaataat taattttaca
    31501 tgatctggtt tattaactga aattagtttt tcaaaaaata gaaataaatg aaagttagac
    31561 tccttgtttt tattgccgta ttggctgttt tatggggttg caatcacgaa aatgaacccc
    31621 aaatttatac agtcagcggt aaggttcaaa aaggaccatt caccacaggc acaaacatta
    31681 ttttaaatga actaactaca agcctggctc aaacaggcag ggcttttacc acaacaatga
    31741 gcacaaacga tggcagtttc agtttaggaa atattgaact ggaatcggaa cttgctcttg
    31801 taactgctac cggatactat ttcaacgagc tttacggaga gttatctccg gcaacgctaa
    31861 cattgagcgc cattaccaac ctgtcggcaa acggcactgt aaatattaat gtaatgactc
    31921 atgtaatacg ggcgcgaacc gaagtgctgg tttcaacagg gatgagtttt cgcgatgcca
    31981 gtgagcaggc gcaatcggag tttcttgctt ttctgaatgc agcagattcg ggttcgcaaa
    32041 gttttgatga tttggatatt tcgcagaata actcttacaa cgccagttta cttgcattct
    32101 cggtcatttt gcagcgatac accaataaag ctctggagaa acctcaacta actgcagaac
    32161 ttacgcagtt actggcaaat attagcaccg actttgcccc tgacgggcag attacgaatc
    32221 aggctatcat cgacacccta ctttacaaca tttcgaacct gaacctttac gacattaggg
    32281 aaaatatagc ccaacatttt gccggattag gaataacggc cgacattccc gattttgaaa
    32341 cctatgttga taaattccag gaattacata gccattattt atatcaggat ttctattatc
    32401 cgacaaatgc ctctcctgaa cccattgttt ttccgcatga tgaagttccc aacctgcttg
    32461 ctttgaacgt agtaaatttc acctcacagg caacgtattg catcgctgca gtggtgccct
    32521 tatattcgtc gttgcgcata caaattacct tcacatcaaa tccttccgga tttacggtta
    32581 cacccggcac acatggctgg cataccgaaa gggacgggaa tgtactgaac atgtattctc
    32641 aaagacgtaa tactctcatg agctatatgg tggggtttga gggaacaggc tctgcgctga
    32701 ttgaatactt cgaaaacggt gaaacatcac cttcgtttac aaagaccatt acatggcaat
    32761 aaaaatctac caatgacgta taaataattt gggaatactg tatgcagaat acagaaggtg
    32821 gcatttggtt agggaagata accctgcatt tccgagaaag aacgcaaact ccatcatgag
    32881 aaggatgctg ctgaattgca agccacctct tctgaattct gtaatcctga gcctcggtca
    32941 tcgagaagtc tagatgccga aggatgtctc ctgtcttctg attcagtatc cctcgtcatt
    33001 tcatattgct tttattgccc caaaattagc cctcagggct tctcagattt gtttgttgtt
    33061 cctgaataca tataatttat actgactttt caattttccg gtttcgggca aatcggtata
    33121 taaaccatac atggcagacc cactcccact cattgaagcg taaaccgctc ccatagagta
    33181 caattctttt ttaatttctt ccaacaccgg atacgcatta aaaacactcc gttcaaaatc
    33241 gttgatcagc gtatttttcc attcatgtat aggtcggctg gcaatagtaa caatactttt
    33301 ctcacggaga taaggtgcgc agtttttata agcttctgcc gttgatacat ggacatccgg
    33361 caacaccaga accagaaagt attcggaaat atcgtatggg taatcaacca aaatctcacc
    33421 tctaccggag gcaacggcag ccgtattctc aataaaaaat gcacagtctg agccaataga
    33481 cgacgcatat gaaatcatct gttcatgagt aagttttagt gcgaacatat cattcagcat
    33541 ccttatagta aaagcagcat ccgacgaccc gcctcccaaa cctgcaccgg aaggtatgat
    33601 tttatgcaga tgcactttta caggaggaag cttgtgatgc aaacaaagca tatcgaatgc
    33661 tttgcggcag agattgtcgc ctgaatcaca atcaagaggg attccggaaa cgcccatttc
    33721 gaaaggtact tttgcattgc cgacaggaag tatttctaat atgtcgcaca aaggaacggg
    33781 cagcatcagg gtttcgatat cgtgataccc atcaggtctt ttgcagttaa tatgaagccc
    33841 aatattgatc ttggcgtttg gatagcatat catagctcaa aaatacgaag aattcagggt
    33901 tttgttagtt attatacgat ttttttataa ctttacatga aatacttttt atgccatcat
    33961 cggtaatcag gtactatttt aaggaagact acagttttat gcaatcaacg ctaagactac
    34021 tggcatggtt tggatcaatc gggcatttgt tgttttattt cgtattacta tatctcgttg
    34081 gctactggga gtcgctaccg gttagaattg ccgcctctgc cctgtttctt agtcttttgc
    34141 tccttccaaa gctgagaaaa ttcaatacat tccatattgt gtattatgag tttgtatata
    34201 ctcttgtttt ccctgtgatg ttccttttct ttttagtgca aaacaattta aacgcctatt
    34261 gggcagcatc ggtgcttttt tcagctgtat tgtatggttt tcttacccat ccacccaagg
    34321 ccttgcttat gtatccgctt agcattgccg taaccctgct ggtacttgta aaactaaaca
    34381 tgcttaatca accggttagc gaagttattt tgattcattt cccggcgtat tttatcatga
    34441 tccttatcgg aattcttcaa acccagattc gctatgccaa tacgaaagcc gaaaaagaaa
    34501 atgcgcggtc cgagagtttg ctaaggaata ttttaccgga aaaaattatt aataccctga
    34561 aaaataaccc tgaaaatatt gctgagaaat acgaagaaac aagcattctg tttgctgaca
    34621 ttgcagggtt tacgcctttt accgaaaaat cgaaaccaga ggaagtggtc gaattcctga
    34681 acgatatttt tacacgattc gatgccttgt cggaaaagta caaaatcgaa aagataaaaa
    34741 ccattggcga tgcctatatg gtcgtcagcg gagcaccggt tgcctgtgcc aaccatgcag
    34801 aagtaattgc agatatggca cttgatatgc tggctgagat aaaagagtac aatgcaagtc
    34861 acagttcgag tatctctatc aggattggca tcaattcagg gcctgttgtt gcaggggtga
    34921 tcggtaagaa gaagtttgcc tacgacatct ggggcgatac ggttaacacc gcaagtcgta
    34981 tggaatcaaa tggtatccct ggaaaaattc agattacagg tgctaccgca gcattgctca
    35041 acggaaaatt cttggtctca ttcagaggag aactcgatat aaaaggtaaa ggtgtaatga
    35101 agacctattt tctcgaaggc aagaattaat ccagctccca gagcactcct attattgtaa
    35161 tcccatttta tataaggcct gaaaatcgcc tgtaaatttt tcacgataca gaaaatagag
    35221 ctgcccatta tgtactttca gattaccaac ccatgcaaaa tcggggatac tgatttcttt
    35281 tccgagggtt ccactatgaa tatatatctg cctcagcttg gtttttccat tcttcagata
    35341 tactgcatac acgttgccgg taacctcatc ggtaaggatc tgattttcga tgtaccggtt
    35401 attctgaaac tgaattccaa ctttacccaa gaaatgaaaa tcgttgttgt accattcaat
    35461 tacagagtcg gtatagttta ttaacgctat agtgtcgcgc aatttaatgg caggagcaaa
    35521 taccgaatcg aacatcatat cttcgaaccg caaatcagca tcgctgggac cattgcagtt
    35581 gcccatcgaa agaaacactc cgtgaaaaac aaacatttca tgttttactt catcaacaat
    35641 tgtacggagc atttcgtagc tttggctcag tgtatcggca taataataca tgagtatctg
    35701 gtttctgtcg ctgtattcgc taaacaaaaa tttgctgcca ctttgtataa tgcagggttg
    35761 aacaatttca agaaattctt tcttaggtga cggatacagc aatactatgc tatctgtatc
    35821 aaaagataat tgaaatgctg tagctgctgt cagaaagtga aggttcccca tgcaatccct
    35881 gaacaatcgt ccatctgcac cagcacaaaa agttgccacc gtgtcgccct gcccgtttac
    35941 cagtactagc cacgggttgc ggtttttatc gtaacgatag caatacccat agtatagtat
    36001 cgagtctccg atgaaagcat aatcgcctac atcaagcatc attccgtaac tgattttcac
    36061 taccggtcga acttccgcag caggaagtat ggtaaccgat ggttcaaatg caacatccat
    36121 ggtaaacaca cccgaagtta ttacctgttg ttgcttaaca tacccaacat gagaaaaaac
    36181 aagagtatcg ccttcgttca cacggatgct gtaagctccc gatgcagcag tggtggcacc
    36241 atttctgagg ttaatattgt aaatgtttac tccatttaca ggattaccgg tttcaatatc
    36301 ataaatactg ccctttaaaa gcactgtatt ttgtgcaaca agcaacacat tgtttgtgag
    36361 caatattatt ggaacaaaaa ttatgtaggc atgtcgcaac cagttcattt ttcaaacaga
    36421 attatataaa attttgcaat tcccatgtca atattatttg ccatccaaat actcatcata
    36481 caaggcatca agttcttttt gtgcctgagc accataatat ccgttttcct taacaatttc
    36541 ttcaagtact ttttcggcag cagcatattg tttttcttcc ttataaataa ggctgctgta
    36601 ccacatcgat tcttcgataa atgtattgat atcagcatta agcaatgcgg taaaactttc
    36661 gattgatttt ttgttattgt tcaaattgta atagcacatt gctttgtaga aaatggcgtt
    36721 gaggtctccc ggaaagtgtt cttcgattat aatgaagttt tcgatagctt cctgatacct
    36781 gccctgctta taaaaaccca gactttgttc aaggaatgcc acgtaagtat acgtagcgtc
    36841 ggcacctgca aattcaatgg ggttcaaata atcagcctgc actttgttgg ctggaatgtt
    36901 attactatta ggttgcctgc gtatttcgaa gcggtagtca accactttaa gcccgcagat
    36961 ataagtatat gggtgattcg accgaaagcg gtacaaatct acaatatcag gttctttggt
    37021 tgtttgctga atcaccatgg atttatttac gaagagtggt tcaatgcttt cggaatgaga
    37081 gctttccttt ggtaattgca gcatttcgcc cgtatatatg gtttctgcct caacccaggg
    37141 agccacggta tcaactacag ggattacaaa gcgcgtttct cctgtattat tggccagttc
    37201 attcccatga ttcgtatttt tcggaggcac agctttcccc gacaatacgt gtataattat
    37261 aaaaacagca acaacagcag cagcgatacc ggaatacagc agcaccttcg tcagcctctt
    37321 tagcgtaggt tttacttttg acggcatgct aataatctca acatcttcgg gtaaagctcc
    37381 gctttgctga aaacctgcta tcgcatcctt caaaaattca tcttctgtca gggctttttc
    37441 gaatgcagct ttttcgtctg cattcattgt tcctttgaga tagctttcaa ttatttcggt
    37501 atggtaatat ttattattca taattttttt caataagttt tttaagttta atacgaccgt
    37561 tctgcaaatg gcttttaacc attttaacag aatagcccgt ttcgccggct atttcggtgt
    37621 acgatttccg gttaaaataa aataacatga tacaggactt ctgctctgac gacaggcgct
    37681 caattgctgc cgcaagtttc tggtgatgct ccgactcggt ttcatctgtc tccgcattta
    37741 cactttcatg tatttggata ttatcgatag ccaactgttc tcgtttttct tttctgagct
    37801 tcatcaagca atggtttttc gtaaccgaat acaaccatga gctaaaattt gtaataacat
    37861 gcagatgcaa tttcgacatg agcatttcga aaatctcgat tactgcatcc agactttcat
    37921 cgcgcgaatt gaaatatttc aggcaaacgc catacaccat gtgagaatac ctgcggtaaa
    37981 attcggcgaa caccatatca gattgcttgc tgcccagcat tttcaacaga tcctgatcac
    38041 ttaggtttcg caacatggtc atgaattaat taaattgtga aaatactaaa aatcgtctgt
    38101 tttactttca acagcttttc gttcgttcat gatatcgaaa tagcctttgg tagtcatcat
    38161 taccacacct ccggttacat ctccgtactt cgaaggaatt cctcctgtgt aaatttccat
    38221 tgagccgata gccagcgaag gtatcgaact catttcgtcc ctaactttaa ccccatcgat
    38281 gaaaaccgag ctgctgccgg ggcgtgaacc gcgaactacc attttattct cataaatttg
    38341 gatctcggga caaatttggc gtgcaaccat gatcggattc ttgggacccg gaagtttcac
    38401 aatagttgct gaaggtatcg gaataagcga tgggttgtct tttttaatca gtttatcgcc
    38461 ataaattacg acttccttct tcatttcggg tttgataagc atggttacgt cgtcgagaaa
    38521 agtaatctga ctgttgtata cagttacccc tgtaacctct gtaacgctgt atcccacaaa
    38581 agagaactca acggtataag ttccggcatc gagtggcttc agtttatatt ctccgtcaaa
    38641 atcggaagag gttcctgcta tggaagaccc cgttttaact actacattaa ccataggcat
    38701 tggtttgccg ttctcatcga gaaccctgcc gtgaattgcg ctgccttgtg ccaataagct
    38761 gcctgccgag atcagcagga tagctaaaat aatgattgtt ttcataataa tgttgtgttt
    38821 ggtgttgttt taccgataga tgctatcaaa tgagtaaatt ccacgcaatc acaaaataaa
    38881 atttttaacc acaggcatat ggttgttatt aagcaagttt gcaattagca atcttcaaaa
    38941 ttaatttgtt tatggctgcc atcgcaaccg ggcatcttct tcgatttgcc acaccggcat
    39001 atacttttgg gttttccggc atacacttca ttgccgttgc tatcgaacaa ccgtacttct
    39061 ttgggaagta taagagggcc atttttaatg attctggctt ttattccgct actattagcc
    39121 atttcttgat cggaaacatt tccatcaggg ctattccatt cccaggtaag tgcgtcggta
    39181 ggacacatat ccactgtttc aataatagcc tcggtggttg ctttcgataa atctacccat
    39241 gggcgcttgc tggggcggaa taccaacgga agttttgcaa agcaaaccgt actgtgaata
    39301 cagagatgag gctgccaaat aaccgtaatg tcatcgttct tatatttttt tacaattggg
    39361 tctgccatac gatacagttt ttggttccaa ggttttctga ggtactgaaa tattgatgca
    39421 tttgtttatt acacttgttt tgttgcaaga tatataaaat acttaatttg gatattgtat
    39481 taacaaaaca atttatcaaa tttgctgttt atatactata tgatatacaa gaagatatat
    39541 tctctattgt ttttgatgct tacgcttgca ggaagcgttg gattctttgc tgttcagcac
    39601 tactgtaata gttgtatgca ctcaggatcg gaattgcaat tattttcggt ttccgacagt
    39661 caccatcata gctgtaaatc ctgcgaagat tccggacaca attgcgactg ccatagtatt
    39721 acgaaaaaag gacaatgcag cagtctattt tttttgatac cggttttttc aaacacatcg
    39781 ccaaacacat ttaagaatat tcagcttgta acaagcttta gcatcaaagc ttttgatgta
    39841 tcggccaaac tccttaaaga gccagaacct ctggaaatat tttttaaccg acaaaaaatc
    39901 cccattttcc taacattcaa tagtcgttca attacttcaa cgggctcttt caggttatga
    39961 ttcattgcat attgtgagta atcattaata tgtagttgaa cttaatctga tttatatgaa
    40021 attaaagacc attttattaa ctccctcatt tataatactg tacctttttg ctcaggcaca
    40081 ggttaccgga aaggtttatt ataaagaagg gcaaaaaagc gtacctctta tcggagcaaa
    40141 tgtttattgg caaggcacac aagaaggcac ggctacggat aacaacggaa gtttcagtac
    40201 cgaaaaaccg gcatcaacaa atgttttgct tattagcttt gttggttatg gaaccgatac
    40261 gattgttgtt aacgaaaaca acaggtatct ggaaataatt ttaaccaaaa cagttttgct
    40321 cgatgaagtt accataggtg agcgaaattc ttcgtcaacc tatgcaaggc ttgaccctat
    40381 cggaacgcaa cagctcactg gtgaggagct aaaaaaggca gcctgttgta atctgggtga
    40441 aagtttcgaa accaatgctt cggtggatgc aaactacaac gatgcgagta ccggtgcaaa
    40501 gcagataaaa ctgttagggc tcagcgggaa atatgtgcaa atgcttaccg agaatattcc
    40561 aaacttctat ggcttggcta cttcctatgg aatggaatat atccccggtt catggatgaa
    40621 ttccatagcc atttcgaagg gaacagcttc ggtgagctac ggttacgaag ccattaccgg
    40681 gcaaataaat acatggtata aggagcctga taaaaaagcc gattatttct acctgaacct
    40741 gcttggtaac acagcaggga aaactgagat aaatatggat ggcagtttta acatcagcaa
    40801 aaaagtatca tcgatgattt tgattcatgg tggatacgat ttttttcagg tcgatcacaa
    40861 caacgacgga tttatggata tgccttctgt aaaaaaatat attctgttta accgctacaa
    40921 tattcattgg agtgatgatt tcgaaactaa aataggggtt aaatatcttt ttgatgaccg
    40981 caccggcggt caactaccag atatggtaaa tccataccag attctaatac gcacgaaccg
    41041 gcttgaagct tttctgaaat caggatactt tttcgcgaat aagagcgctg gcagtgttgc
    41101 ctttatttcg ggctattcat atcacaacca gaatggcgtg tatggaaaca aactttacaa
    41161 tgcttttcaa aacagtattt atacaaatgt gatttacagt accgaactgg ggtcatccga
    41221 aattcatagt tttcagtccg gtgccagtta taaatacgat gcattcaatg aaaatctgaa
    41281 tgattctgta agtctcaggc aggaattggt tcccggtgtg tttgcagaat atactttgaa
    41341 gccttctgca gctttcactg gagtactcgg aatgagggtg gattaccaca atcttcatgg
    41401 atttttctat actccgcgaa tgcatatacg ttataaagtt accggaacaa ccacacttcg
    41461 ggcaacagcc ggcagcggaa ctcgtactgc caatgcactt gcagagaatt catacttgct
    41521 tgcaagcagt cgccgcatcg tattcagtga agagctgaaa cccgaaaaag cgtggaatat
    41581 tggaggcagt gttattcagg aatttccaat tggaaacaga acgcttaccc tcattgccga
    41641 tttttatcgc acctggttta taaatcaggt agttatcgac atggataccg atgttcatga
    41701 ggttaatttc ggcaacctga agggtaattc gtactccaac gtcggtcaaa ttgaagttca
    41761 gtatcctttt atcaaaggta tggattttac cgctgcattc cggtataacg atgttaaaca
    41821 aacgattggc gacatactga tgaaggtacc gttcacatcg cgctacaaag gcttgctcag
    41881 cctgtcgtat aaaacccctc tgaaaaagtg gcaatttgat atcaccagtc agttcaacgg
    41941 cggtgggcgc ataccatcta cagcccaaaa tccggtttac taccaaagag ctgaccattt
    42001 tgatccgtac accattttaa acgcgcaaat taccaaatac tttaaagttt gggaaatata
    42061 tgccggcgtc gaaaacctta ccaattttgt gcagcacgac ccgattattg cctctgaaaa
    42121 cccatggggc gaaagcttcg acggggcaat gatatgggga ccggttcacg
//
LOCUS       DAOOQP010000012        37370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1281_length_37370_cov_6.495270, whole genome shotgun sequence.
ACCESSION   DAOOQP010000012 DAOOQP010000000
VERSION     DAOOQP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_37370_cov_6.495270"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /gene="glnS"
                     /locus_tag="PLK75_02430"
     CDS             <1..1347
                     /gene="glnS"
                     /locus_tag="PLK75_02430"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOY38134.1"
                     /translation="IMLIKKGKAYVDEQSPEEISRQRTLPTEPGIESPFRGRSVEENL
                     LMFDRMKNGEFENGSMVLRAKIDMNSPNMHMRDPVMYRILKQEHHRTGNKWNIYPMYD
                     YAHGQSDYIEGITHSICTLEFEVHRPLYEWFLNEIAKGEYRPRQIEFARLNLSYTVMS
                     KRKLLELVKGGYVNGWDDPRLPTISGLRRRGYTPESIRVFAERIGVAKRENVIDVGFL
                     EHCIREDLNKKARRLMVVLDPIKVVITNFPEEDAEVLEAINNPEDPEAGTRNIYFKRE
                     IYIERDDFMEVPNKDFFRLSPGNEVRLRYAYFLRCNEVLKNPDGSIHSLMCTYDPMSK
                     GGKSPDGRKVKSTIHWVAAEKAIDTEVRLYDRLFTVPDPDNVPEGGDFKDFLNEKSLK
                     VIIAKAEPSIELVAISEKLQFERKGYFCCDSDSEKGEPVFNRIVDLKDSYPKTINK"
     gene            1351..1812
                     /gene="coaD"
                     /locus_tag="PLK75_02435"
     CDS             1351..1812
                     /gene="coaD"
                     /locus_tag="PLK75_02435"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOY38135.1"
                     /translation="MQRIALFPGSFDPFTVGHESIARRALPLFDRVIIGIGTNENKTD
                     FFPLEKRIQWIKNLFADEPKVEVKTYKRLTIDFCRETGASYLLRGLRTSADFEFERAV
                     GQVNKLLDPEIETVFLLTKPEHSFISSSIVRDVYKNGGDISSFIPKGMKLR"
     gene            complement(1835..2413)
                     /gene="lpcA"
                     /locus_tag="PLK75_02440"
     CDS             complement(1835..2413)
                     /gene="lpcA"
                     /locus_tag="PLK75_02440"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOY38136.1"
                     /translation="MANDVIKTQFLQAQTLLAEFSENPENFERIAKAGKLMAEVLQNN
                     GKIISCGNGGSMSDAMHFAEELSGKFRNDRPGLAAVAISDPAHITCTANDFGFDQVFS
                     RYIEAIGKPGDILLAISTSGNSQNIINAVRAAHCKGMKVVGLTGKDGGKIGPLCDIEI
                     RVPWNEYSDRIQEIHIMIIHNIILTIEKELYL"
     gene            2583..3671
                     /locus_tag="PLK75_02445"
     CDS             2583..3671
                     /locus_tag="PLK75_02445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY38137.1"
                     /translation="MKPYFKGLDTLRAVAALIVVIGHVEEIKNIFGIPNILHSKGFQL
                     PDGHLSVVLFFVLSGFLITFLLIKEKQNFGKISLRDFYIRRVFRIMPLYFMVLFLSFV
                     IFRQEYQTNTIILCATIFPNIAHALSIGWPTSPQIWSIGVEEQFYLLWPLLLILIPPK
                     RVTIYLILFFAGFSLIHLLLGYANFNTVQNTGFNDFVYKFFYFSKFNSMALGSMLGYM
                     CATNNKNLRFLFNNYVAYASVFLSFFMWFFHISFQYFNDEIYSMVFGVMIINLAANNS
                     FKLHIDTKVSVFLGKISYGIYMYHWIIIMLLMKLIPYSIFKTNISFNIVIYSLAIILT
                     ICVSWVSYVSFEKYFLKLKERFTRNTAS"
     gene            3798..6602
                     /locus_tag="PLK75_02450"
     CDS             3798..6602
                     /locus_tag="PLK75_02450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY38138.1"
                     /translation="MRKLHYLLVLVLSVFAVNAFSQGLDYNEKLIFDPGVRVGKLDNG
                     ITYYIKANATPQKRAELILVVNAGSVLEDEDQQGLAHFCEHMAFNGTRNYPKHELINY
                     LESLGMKFGPEVNAYTSFDETVYGIKVPTDSAEYLDKGLLVLYDWAYQVSFEDDEIEA
                     ERGVIHEEWRMGQGAMDRMQRKMLSTLFYGSKYAERLPIGLMSVVDSCPHDVLRRFYS
                     DWYRPDLQAVILVGDFNADDVEAKVKQLFGKIPAKDSPRLREVIEIPDHEQTLVSIAT
                     DPESPIGMVYVVYKHPLKSTTTVGDYREDIIAGLYNQMINKRLQELTLLENPPFAQGA
                     SMYTSFLGPKSVYMSIGVAQNNDIGKALEAVTIENVRVQQFGFTATELEREKAAMLKE
                     VEKQYNERAKQKSEDLAQEYQRNFLPPYEPVPGIEKEFELYKQLLPTITLDDINNFAK
                     TLVTKNNTVISVMMPEKDGVVIPTEEQILEVYNKSLNAQVSPYIDKVSDKPLLAELPP
                     KGKVAKKAKNKDLGYEVWTLSNGAKVVIKQTDFKEDEIRFHAQSWGGASLYEQDADIS
                     ADIAADVAIESGLGDFDKTELDKYLSGKNVYLTPSISELSESLNGSCSVNDFETLLQM
                     IHVSFTKPRVTESAFNSYINKQKGLLANAMLDPQSAWSDTLQTTRANNHPRRRALTPA
                     NLDEANFKMAQKIVKQRFGDPSNFTFYFVGNIDPKSSKELIEKYIGSLPTVTRNESYK
                     DMGIRAPQGKVEKTVYKGKDNKCMVVMDYNGTMNYNAKNKIELNALCSILSTKLLEEI
                     REKESGVYTIGAYPQVQKHPVPRYDIVIFFSCDPARLEELTTKVIAEIDKLKNNGPAE
                     ADLLKSQEKLRREYETNSRENSYWIGKLSEIECGDLSVEEFKNFNNYVNQISQESLKT
                     IANTYFNYNNYIKVVLKAEK"
     gene            6800..7636
                     /locus_tag="PLK75_02455"
     CDS             6800..7636
                     /locus_tag="PLK75_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38139.1"
                     /translation="MPQHRLPGSDFLRFKALKCIAEAVRNDINPDRFISVDKIGKNQQ
                     YYNLMEKVLEYNTINQKLRIEHQTEYNNLFSKAKMYVLHYLQIIDMAIERGEQPISVR
                     NYYQLDESGGKLPQINNESELVKEATNLFTGDAKRTSEGGKYFTNPTIGLVKMWFDKF
                     MEASQKEKNLSFVKTGEVENIALLRKEINCFIDEVWSEVEKNSAARGREEQLKICHSF
                     GIEYEMTQEDVLIGKQEDVFKEPGLFSSMQGDCEDRLMKEACHESKSESLNLQFAFEF
                     PK"
     gene            complement(7607..8515)
                     /locus_tag="PLK75_02460"
     CDS             complement(7607..8515)
                     /locus_tag="PLK75_02460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOY38140.1"
                     /translation="MTHNDQILKNFRVIALTILIIALGFILAQITTAIVLIHGGVTDI
                     NSLTGPNPDAGALGILKLGQFFSSFFSFLLPAIIVAWLIYRKPAERLKLNSVCHIAWY
                     VLPVVILFFSLPWMNMVIKWNEAITFPECMSGLYHSLQDSEQMAKKMTELILSGNSTY
                     TLLINILLVALLPALAEELMFRGIIQSLIHDLTHNKHVAVLLSAILFSAIHFQFFGFI
                     PRFLLGAFFGYLVMYSGSLWPAILAHFFNNAMAVIVYFYINKGAIQKDLEDFGSNSSD
                     IMFTLISAMITIFLIAILFRKFKSEL"
     gene            complement(8512..9546)
                     /gene="ribD"
                     /locus_tag="PLK75_02465"
     CDS             complement(8512..9546)
                     /gene="ribD"
                     /locus_tag="PLK75_02465"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOY38141.1"
                     /translation="MGYETYMKRCLILARQGLGLVAPNPMVGCVIVKNKIIIGEGYHH
                     RYGGSHAEINAINSVKDKSMLAGSTLYVNLEPCSHYGKTPPCSEAIINNNIGEVVVGS
                     VDPNPLVAGAGIARLKASGIKVNFGILDEENKELNRRFFTFHQQQRPYIILKWAQTSD
                     GFLDRVRKSPTTEKPVWITNEMCRILVHKWRTEESAFMVGANTVLLDNPMLTARSWFG
                     KQPLRITIDAEGVLSKELNIFNNKAPTLLFSSEIRKGIDTEIIDWNKNIANQITERLY
                     QRNIASLVVEGGRKLIDLFIQSNLWDEARVFSGPAEFGQGVPAPEITGDVIREISIGN
                     SILRIIRPAK"
     gene            9712..10638
                     /locus_tag="PLK75_02470"
     CDS             9712..10638
                     /locus_tag="PLK75_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOY38142.1"
                     /translation="MNVLLVILGLLMLVVGSELLVRGGVSIAKRVKISPLIIGLTIVS
                     MGTSTPELVVSVKAALQGYPDMAMGNVLGSNIVNISFILGLTAVILAIPVNRETAVFS
                     WPVLLLATVLLFGFSCDFKLARWEGIVFVALQVLFIVALYFRSKRKKQQDEEDSGKTY
                     SIVVSILMLIASIAGLSFGAEFLVKGAVGLATHMGVDERIISLTIVAIGTSMPELITS
                     VVAAMRKQMDISIGNLIGSNIFNIFTILGVSSAITPITVNPQSLYVDMTWAFGLVILL
                     GLCFIPLRNSVVKRWKGLLLVVAYMAYFFYIF"
     gene            complement(10664..12334)
                     /locus_tag="PLK75_02475"
     CDS             complement(10664..12334)
                     /locus_tag="PLK75_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOY38143.1"
                     /translation="MAEANVLSTNFDVFRNENILLGTKVLLIYPPVRLNKKPLYPPFG
                     LLSLAAVLEKAGAAVEILDLNMLRLGFTDLKHEIAQHQFDVIGTGGMATVYYYMKFLA
                     EYLKKDYPNVPLIAGGTACSGSPDVMVKHTKFDVIALGEGEPVIIDIVAALTGKGDLN
                     TVPGIIFRDKTGNVIKTADRPRMLNLEDMPLPAYHLIDMEKYISNSYIYKNKKNSVSE
                     ERIKKFNLDRHKASRPVMLFSKRGCPYECNFCYRNFGRKVVGLSVDYTLKHMAFLEEK
                     YNTINFIIEDETFNIDRKWVMEFCDALIASDKKYILNIGNGLRANSVDDEMISRMKQA
                     GFCSIAIGIESFYNPSLHDMNKKQDADTIINAIQVIQNNGFHLASAQLLFGYPSDGTE
                     SMQENITWCKALNLKSANFAIPCPYPGTLLYRKAIDEKYITDEEAWLMELADKDISDR
                     VINMSGKPEKHLKKLILRAEDEIRMHFIRKDYPVIGHIVSWMQKAGRAFNLNAFEILK
                     GIKDGAKNLILYGKIPGKLFKSGGTNHSHIKTEAMYWLHEWGKTSAYK"
     gene            12490..13368
                     /locus_tag="PLK75_02480"
     CDS             12490..13368
                     /locus_tag="PLK75_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38144.1"
                     /translation="MNQTFKQVNMWIKRLNQSLLSLIKVFLYSKIPEVFNRAVVNEAV
                     ILGNGPSLSGMISENAEFLKGKELFAVNYFALSDDYHTLRPRNYVMIDFTFYYNTIGD
                     EESILKRDKLWQRIASFTTWEMNLYLPFNAKKHSYWKKFVCENKNIRVKYINVTAVEG
                     LRGLRNYFFSKMLGMPRPHNVIIPSLMIAVKMGFKTIYLWGVDHSWLANVSVDENNVV
                     FVNNKHFYDFNTSKPGLYILKNTPAARMHEMLYSWMCTFESYFIINDYASKYNVSIIN
                     QTPNSLIDAFVKKRIE"
     gene            complement(13371..14864)
                     /locus_tag="PLK75_02485"
     CDS             complement(13371..14864)
                     /locus_tag="PLK75_02485"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOY38145.1"
                     /translation="MGIIVKQSVKGSAYSYIGTLLGFINVGLMMPLIFSKEQIGLTNL
                     LVSVSAIIAQLGTLGFSNSIIKLFPFFRDKEHSHHGFVKVMTLVSILGFALTFVLFLF
                     LKPYLIESNIEKSPLFIDYIWYLPPMILITIFYLIYDSYCIVLFNASIGIFYKEFLMR
                     ILITAGIVLYYFSVFDFAGFVLFYFLAYSVPTIGLLAYLRLKGEYNLKTDKGYITKPM
                     RNELIWVSFYGLIIGFSGIAVMNIDIYMVNHYCGLAGAGVYSTMFYFGTIILIAGRSL
                     KRIASPLISEAFKKNDTALIGEIYTKSTLTQFIISIYLFLLIWSNIDSAFEIIPRYYS
                     EGKMVVFYIGILQVLTMLSGASYEIIQYSKYYRLYAYLLLVFIAMVIASNMFFIPRMG
                     LTGAALASMSSFIVFAVIRFFFIKSKLGLQPYNLRHLYALIIGLVVFIILHFIPDAPN
                     FIIDIIIKAGITTVFFVGPVYFLNISPDIRNAIKNYFKAGIAVFVKK"
     gene            complement(15014..15436)
                     /locus_tag="PLK75_02490"
     CDS             complement(15014..15436)
                     /locus_tag="PLK75_02490"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOY38146.1"
                     /translation="MKRKTLKFSPEYNFILFGLSATVSDIRLVYILKTMTELDFHRCE
                     NLCVFHEKLPIEQTFSLFECDENDENLSISLISNKSQEGFLIEELKNIDYFIIIKGAD
                     SIVFAEKFLKVLKESSSIYGVFQIDPNNLPSKQKLLFQ"
     gene            complement(15438..16181)
                     /gene="rnc"
                     /locus_tag="PLK75_02495"
     CDS             complement(15438..16181)
                     /gene="rnc"
                     /locus_tag="PLK75_02495"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOY38147.1"
                     /translation="MFLKTRFSSFFNRKTKAYSYQLYKLLGFYPKDIVIYQTAFRHIS
                     ASLNSPDGVQINNERLEFVGDAILDAVVADILFTAFPEADEGNLTMMRSQIVKRKSLD
                     YLAHKIGITDFLIYRSKSENDKNSGHIAGNALEALIGAIYFDQGYLRCHSYISAIIKT
                     HINITTLNKDNEDYKSLLLQEMQRRRHSIVFDSYENIEDCEKEIHYSCAILVNNVFIA
                     EGKAWTKKEAEQEASRIAVRLLLKADKTC"
     gene            complement(16185..17432)
                     /gene="fabF"
                     /locus_tag="PLK75_02500"
     CDS             complement(16185..17432)
                     /gene="fabF"
                     /locus_tag="PLK75_02500"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOY38148.1"
                     /translation="MELKRVVVTGLGTISPLGNNVSETWNNIINGVSGANLVTHFDTT
                     NFKTKFGCEVKNYDPANYFDRKESRKYDFFTQYALISSTEAVNDSGLDLDKIDKTRAG
                     VIWASGIGGVEGLIQELKGFFDGNCIPRFSPFFIPRIIIDIASGLISIKFGFMGPNYG
                     TVSACASSSNAIIDSYNLIRLGMADVIVTGGSEAAVNEIGIGGFNALQAISTRNDDYL
                     TASRPFCATRDGFVLGEGGAALILEEYEHAINRGAKIYSELSGVGLSADAYHMTAPHP
                     EGLGASLVMKNALKDAGIEPSCIDYINVHGTATPLGDIAELKAIKGVFQDHAFKLNIS
                     STKSMTGHLLGAAGAIEAMFTILAVKNNIVPPTINHHEKDPEIDERFNLTLHKSVKRD
                     IEYGLTNTFGFGGHNTSIIFKKA"
     gene            complement(17459..17695)
                     /locus_tag="PLK75_02505"
     CDS             complement(17459..17695)
                     /locus_tag="PLK75_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOY38149.1"
                     /translation="MSDIASRVKSIIVDKLGVDEAEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFEISIPDEEAEKISTVGAAIKYIEENTN"
     gene            17850..18425
                     /locus_tag="PLK75_02510"
     CDS             17850..18425
                     /locus_tag="PLK75_02510"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOY38150.1"
                     /translation="MKKIAIFASGTGSNALNIIGYFRQNPTISVTDLLCNKKDAPVIE
                     KAKNAGVRTFLFGKEEFYNSDVVYDYLVGHNIDVIVLAGFLWLVPEKIVNAFPYRILN
                     IHPALLPAFGGKGMYGSKVHAAVLKSGDPESGITIHFVNVHYDAGSIIHQSKCSVNDT
                     DTPESLAAKVHELEHRYYPGVIEQVALGLLP"
     gene            complement(18466..19095)
                     /locus_tag="PLK75_02515"
     CDS             complement(18466..19095)
                     /locus_tag="PLK75_02515"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOY38151.1"
                     /translation="MKKRATLISIVLIFAILLIDQIIKIWVKTHMTIGEDISVIDGFF
                     SIQFIENMGMAFGMEFWGKWGKLGLSIFRMIAISGLVWYIVKLIKTQAKLLFIICISM
                     VIAGATGNLIDCAVYGLIFNESTTLQVASIFPPEGGYAGFLTGKVVDMFYITAKIGDT
                     SLFPFIFNIADASITISVLIMIVFYNRVFGIKKQTIAEPVEHDEHCIKE"
     gene            complement(19162..19542)
                     /locus_tag="PLK75_02520"
     CDS             complement(19162..19542)
                     /locus_tag="PLK75_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOY38152.1"
                     /translation="MAEEKKRYSKAELEEFKEIILEKLEKAKLDYDLYKNALNHGDTN
                     DTADTSPTFKVLEEGAAVLSKEEAGRLAQRQLKFIQHLQAALMRIENGTYGICRETGK
                     LISKERLRAVPHATLCIDAKNNQK"
     gene            complement(19559..23029)
                     /gene="ileS"
                     /locus_tag="PLK75_02525"
     CDS             complement(19559..23029)
                     /gene="ileS"
                     /locus_tag="PLK75_02525"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOY38153.1"
                     /translation="MSKKFPEYKALDLPQINRDINELWDKENTFAKSITTRQNCKEFI
                     FFEGPPSANGKPGIHHVMARTIKDIFCRYKTLDGYKVKRKAGWDTHGLPVELSVEKTL
                     GITKEDIGKKISVEDYNKTCRTEVMKYTREWEELTREIGYWVDMDNPYITYDNRYIET
                     LWYLLKKLYEKGYLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTCVSMFQVIR
                     NEKSSFLFHTPDDEISILAWTTTPWTLPSNTALAVGESIEYSLVETFNPYTGNRIKVV
                     LCSDRISAYFKPEGLQLDFSTFKQGDKVIPYRINNTFPGSQLIGIAYRQLIDWVKPMT
                     NAFRVISGDYVTTDDGTGIVHIAPTFGADDLRVAKAAGIPALLIEDKDRNLQALVDKQ
                     GKFFRIEEMHPDFLKEYVNTASYSTFAGRYVKNEYDDNANETTVSADIEISVMLKQNN
                     KAFRIEKHIHNYPHCWRTDKPILYYPLDSWFIRTTAFKDRLIELNKTINWKPASTGEG
                     RFGKWLENLVDWNLSRSRFWGTPLPIWASSDGSERICIGSVEELCTGIKASIQAGYMK
                     ENPFEGFIPGIFSDENYARIDLHRPFVDKVVLVSESGKPMYREPDLIDVWFDSGAMPY
                     AQWHYPFENSERFNELFPADFIAEGVDQTRGWFFTLHAISTMISDSVSFKNIISNGLV
                     LDKFGNKMSKRLGNAVDPFEVIEQHGADALRWYMITNAQPWDNLKFDVEKDPETGKNI
                     YAGIDEVKRKFFGTYYNTYSFFALYANVDKYTASEKEIPVSERSELDRWIISLLNSLI
                     IEVREHYENYEPTRAGRAIQTFVADHLSNWYVRLGRKRYWGGDFSEDKISAYQTLYHC
                     LITISKLASPIAPFITDRVYRDLNQTTGKESADSVHLCSFPVADANCIDKYLEERMQM
                     AQDISSMILSLRRRVNIKVRQPLQQIMVPVLTDSFAEQLRLVESIILTETNIKEIKYL
                     TDASNLLIKKIKPNFKTLGPKFGKLLKPISEIIQSFSQEDINRFEKDGFINIQHEGKE
                     IRIERADVEILSEDIQGWLVANEGTLTVALDITVTPQLKQEGIARELVNRIQNLRKDS
                     GFEVTDKINIFVQHHSELSDSVINFNDYICAQTLAHSINILNKMEHNSSKIVEIDNFE
                     TLIRVERIGK"
     gene            complement(23172..24407)
                     /locus_tag="PLK75_02530"
     CDS             complement(23172..24407)
                     /locus_tag="PLK75_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOY38154.1"
                     /translation="MKKPLQYWGIFISLLFLFNNLPAHSITGGEKGKKSLTMHIDSML
                     NSVKPEKSFIHHDEVLFSDSLVYPFEVYRLRIEVLGRTTLIPLQYNEYVQKYINAYTL
                     PNRDKLNRIAGLSEYYFPLFEEYLAKYNLPLELKYLASVESALDPNAVSPSGAVGLWQ
                     FMKPTADLFDLQVSSYVDERRDPYRSTDAACRYLEYLYRTFGDWLLVLAAYNGGPGMV
                     KKAIIRGGSNDYWQIRQYMTEQMQNYVPAFIAMCYLMNFSREHQIIPLKSDFDFFDVD
                     TIHVFGPLNLVNLANEMQFPAENILLLNPTYYRNFIPSDGKEYALVLPHEYISKYLEI
                     VSDSLSQSTKNLRNTMVVTSGTSSTQVPVQHVVKKGESLHRIAITYGCTVDEILKWNK
                     LPDNHQLLAGETLTIILKK"
     gene            24554..27319
                     /locus_tag="PLK75_02535"
     CDS             24554..27319
                     /locus_tag="PLK75_02535"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HOY38155.1"
                     /translation="MKKKTLIIIAGIVLVVAIGAIVYFVFFSRKDVVVSESIKAIPAN
                     ACIVIQIRDVNKTYEGLKEKSKIWNELSVLPVISRCDNFLSRISKMRGTEKIVEDLLS
                     NNSLFISVHPVGKSKYEIFHAIAIPSDINPEDLIALFKKEYADSAVCSERSFENVTLY
                     ELKYNIGKRSLCFAIAGGHLIWSYSPLLVEESVNQMKGKKKSLADNEDFTTIYKSAGQ
                     KELANIYLQIPSLPLLLGTQLNADASLLKDAVVGFAGWTELDLGIDDTRFTFSGFTAV
                     NDSLLGFVNVLQSQKPAPMTIETVLPANTYFYMQMAFTDANAYADNLIKFLKFTEQNI
                     NYEKLCKQAENEYALNLGELIYPLINREVGLAMVESSNGEDEIYSYLIINTKSRSIAE
                     NELERICEKISGSAKYKSVINIDEAVEFNVYRMDFPDIGDVLFGPVFSGLNSNYAAFY
                     DNYLIFGNSVAAIDEFLRKALLNKTMATDLKHAGFMSGFSKNSNMFVYVNPMRAKNHI
                     CKILTEEALHIFQNSFQSISRISDIGLQWSVSEGMLYNYAMIQFDPEQREEPKTIWTS
                     KLDSIVLNKPVIVVNHLNQSKEIIVQDRKNILHLISESGREIWKIPVDGRINSEVYQV
                     DAFNNGKLQYLFSTETQIHLIDRLGNECDRFPVNLREKSNTGISLFDYENNRSYRIAV
                     ACNDKKVYLYDIEGKLVKGWMFETSDNPVLQSPQHFRVGSADYIVFNDLYKLYVVDRK
                     GQTQAAPSTGFLFSENNDVFFDATGQERFIASAKNGTVYFMYLNGKTDSISFDSYSEK
                     HFFNYYDIDNDGKKDFIFIDGGSIDVYKADKKKLFSVNLNSDINYPPYFYKFSRNQIK
                     IGVVCKDKEQIYLLNDDGTLFEGFPLRGISPFSIGYLNNNSGSFNLVTGGKDNSLYNY
                     EIKESSE"
     gene            27361..29028
                     /locus_tag="PLK75_02540"
     CDS             27361..29028
                     /locus_tag="PLK75_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38156.1"
                     /translation="MKRFLISSFIVGFLIMLFNSCIKDNFDFDKWDGEVEWTPSFVAP
                     VAWGDLSILEAIEAYDSTINLIANTDGYVSLLYYTNVSSRDISELIVIPDQAYFGVVN
                     GTDIDFSEFDQNGEILSGTVNLKMGFTMFNEECEIDTIYVKSAILNSLVQSTYKHTLQ
                     LKFTFPTIVKNGSPLQIEYFYAPVGGIDANNDISLQGYHIDLTQTALGYNEVPVVMSY
                     IFYHSGTPDNSGNIIFNHNFEDLEYEFMSGYFGYNSIIFETDSVNIGIFKNKNLNVDE
                     YLFQDPKFKVRYHNSYGIPTNFFFTEMYARSKINDQNYNIIDYENGLPLDSLHPYFVN
                     FPNIPWFMRDDSIVLNRFNSNIAQVISIQPKWIQFVAHAHTNPNGTSHTNFITDQSVL
                     VADVEVELPLWGYIDNFHAADTSDFDFSEMYSTAQAIKRLAIRLDMTNGFPVEGYGQV
                     YFMDENYTILDSLLYSYQERIIPAATIGSEGQVLEYAKKVTIVEITDDRVEKIKDAKY
                     ISYEGHSNSTEYYDDRLVKIYGEYRIKFDLSFEADIEMNVDIDTIEF"
     gene            29032..30465
                     /locus_tag="PLK75_02545"
     CDS             29032..30465
                     /locus_tag="PLK75_02545"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOY38157.1"
                     /translation="MKKLVIISAVVFMAINSVAQQDFSLYYMNRTLQSQWLNPSSDYP
                     YAVSVGGLLVPVFGQISPPLHFNYANNAWHYNDILHMGTGIKADSLVLDIDKMMNNVH
                     KQNNIRFENYIELLSVGFKVKTLFFTFSISEKLNYGVSIPGDFMHLIVYGNMPYYSDN
                     RSYDFSRLNLNFTHYSEYALGVSASADKLRIGGRMKLLFGKSNISTDIKTLSLYTNPE
                     NVYMTAVTDMSIRASQPFPIEYEYYPDGDSIRFDLPDDLGSYFDPADYLLNPKNFGLA
                     VDIGASYEINHQWQVFASATDIGLITWNDNPFSLVSNGSFLFKGIEADYWHSTDEIEE
                     SLENLGDSILRTFRFEESNQGYVTFLPSNLYVGGMYKPFDKIHIGALYRAEFYKKSIM
                     SSLTLSFNSNLTKWFSGHLSYSIANNSFINFGLGFTLRLAIFQFYLTTDNLTGIIWPQ
                     KSQNMNLRMGCNMVFGYKEKPVSKIFY"
     gene            30493..32067
                     /locus_tag="PLK75_02550"
     CDS             30493..32067
                     /locus_tag="PLK75_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY38158.1"
                     /translation="MELNRLTRSDIHRYVYLLGLLIIAAFLPFSVYITSVAHFVIIAN
                     YLLEGNYYQKIRILFARPSVVLFLLLIVVHFIWLINSTDIHYALKDIRIKLPLLALPI
                     IFGTTAPIKFRQLKLILLVFAGSILLNSVISTLSLAGVFATEQLNDTRDISLFVSHIR
                     FSLMIVLSIFILIWFMFRRKIVYRFEYVLYSTVALWLISFLIISQFFTGIVLFFVVCI
                     LFLLWVFLQQKRRILMISGIITLFLLLVSFATLGYFQVRSFYSPPVRSGTSLLKTTEN
                     GNPYYHNMSSRLIENGNLIYVEICENEIRGAWELRSTLSIDSLDAKQQPVIHTLIRYL
                     SSKGMPKSGNAVLSLSDEEIKAIEAGKSNVRYLDGIDFFDRIYQFIWQIDCYIKEGNP
                     SGHSLTQRIEYVITGLYILKNNFWFGVGTGDVDSVFKQAYIEKNSMLEEQFRHRAHNQ
                     VLTFYIAFGCIGATLCLIAIFFPLILESKKSRFLLLLFVLIALVSMLNEDTLETATGS
                     AFFAYFYSLFLWGVNE"
     gene            32086..32559
                     /locus_tag="PLK75_02555"
     CDS             32086..32559
                     /locus_tag="PLK75_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOY38159.1"
                     /translation="MIDNNQHYMQFAIEMALKNLKSGGGPFGAVIVKDDVIIASSGNS
                     VTVDNDPTAHAEINAIRKAAKVLNSFDLSGCTIYSSCEPCPMCLGAIYWARIDTLFFS
                     ATRVDAENAGFSDAMIYNEIGMPIHKRTLQTEHLVTENAELPFEEWKNLLNKTLY"
     gene            32526..33068
                     /locus_tag="PLK75_02560"
     CDS             32526..33068
                     /locus_tag="PLK75_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOY38160.1"
                     /translation="MEKSAQQDPVLKRLPLCFYTRDAKTVAIELLGKTIVRRHSDGEI
                     VKYIITETEAYLGEFDLACHASKGKTPRTEIMFTQGGVLYIYLIYGMYWMMNIVTGFE
                     NQAQAVLIRGIDKASGPGKTAALLQLSKEFNGESLLESNRIWLEDSNLVCRYKATPRI
                     GVDYAGEFWKSQLLRFCAIV"
     gene            33217..34827
                     /gene="ricT"
                     /locus_tag="PLK75_02565"
     CDS             33217..34827
                     /gene="ricT"
                     /locus_tag="PLK75_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOY38161.1"
                     /translation="MIDREFERGCQIDILDDNTGIINKCCYKLNVNNWLNDIPVATSG
                     FDVVEVRFKNTRKGFFRNVNQLNLREGELVAVESTQGHDIGIVSLTGELVREQMQRYH
                     VNYDSDFKKIYRKAKQTDLLKWKEAVAQEIPTMLRTRELAKQLQLNMKIGDVEYQGDK
                     TKAIFYYIADERVDFRQLIKHMAEEFKIRVEMKQIGARQEAGRIGGIGPCGRELCCTT
                     WMTNFISVSTNSARLQEISLNPQKLAGQCSKLKCCLNFELEVYIDARKDFIYDNKTLH
                     TTQGDAKHIKNDVFKKTMYFEIFNNNIPVVIALPLQRVKEIIELNKKGIAVDKLISEE
                     NKAPSVTEHEFTNDANNGSITRFDSKNRQSNKKSHKKNMKPNNPATPLMLAIVFLISS
                     FSISCDRNKIYEKYIKIDNMVWNDKNYIRFEKEITDTVSRHNIYILVRNATQYRYGNL
                     WVFVSTTAPNGNKDTDTVECVLADKNGKWLGDGMGDLWDNEILWKRNVTFPYQGNYVF
                     QFEQGMRIENLPGILDVGLRIERVPEEK"
     gene            34843..35559
                     /gene="trmB"
                     /locus_tag="PLK75_02570"
     CDS             34843..35559
                     /gene="trmB"
                     /locus_tag="PLK75_02570"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOY38162.1"
                     /translation="MGQKKKLKRFAENNLFSNVFQPLYTDLINDDFGLKGNWKQQFFK
                     NNNPITAELGCGKGDYVTALSGLLPLNNYIGIDIKGARIWKGAKYAEDKAVQNVAFLR
                     TRIECSPRCFEKNEINEIWITFPDPQLNRKKRIKKRLTSARFLGYYQKILINNGIIHV
                     KTDDDDLFAYTKKVIEFNNLTLEKEIRDIYKECPEDKILSIRTYYETKWLTEGRTIKY
                     LCFRLPDTRDIVEPDTFDED"
     gene            35549..35887
                     /locus_tag="PLK75_02575"
     CDS             35549..35887
                     /locus_tag="PLK75_02575"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOY38163.1"
                     /translation="MKTDSHNDFFEKVWAVATLIPYGRVTSYGAIARYLGLPGSARIV
                     GWAMNQSHMNPEVPAHRVVNRQGFLTGKHHFFSESLMAELLTNEGIGVENDRIVDFKK
                     HFWNPSQELI"
     gene            35924..37030
                     /locus_tag="PLK75_02580"
     CDS             35924..37030
                     /locus_tag="PLK75_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOY38164.1"
                     /translation="MIVSIEKIREELSKLQHPGKSTDLVSLGMVQRIVVKEQKIEIGL
                     VFDKATDPFMGSIEKKCKEVVAAMAGDNFTITVGSSVKKPVAPKKKEHAEGLEVNNII
                     AVASGKGGVGKSTIAANLAVALSIDGYKVGLLDADIFGPSIPKLFGLEAYSPSVEKID
                     GRDMIIPAEKFGIKILSIGFFVDADNALIWRGPMASGALKQLITDTAWGELDFLIIDT
                     PPGTSDIHLTLVQTIAVTGVVIVSTPQQVALADARKGISMFRSEGINVPVLGLVENMS
                     WFTPAELPENKYYIFGKEGVKKLAEEQNIPLLAQIPLIQSICEGGDQGRPDHALGHDA
                     LAEAIRNMASETVSQLQKRNANLPPTQIVEINSK"
     gene            37038..37268
                     /locus_tag="PLK75_02585"
     CDS             37038..37268
                     /locus_tag="PLK75_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOY38165.1"
                     /translation="MEEVKKRIQKSIDEIRPYLRADGGDISFVDFTDDGVVKVKLLGA
                     CGCCPHSIQTLKLGVEAKLMEDVPEVKEVVAV"
BASE COUNT        11370 a         6987 c         7622 g        11391 t
ORIGIN      
        1 atcatgctga ttaaaaaagg caaagcatat gttgacgagc aaagccctga agaaataagc
       61 cggcagcgta cgctacccac tgaaccaggt attgaaagcc cgttccgggg cagaagcgtc
      121 gaagaaaacc tgttgatgtt cgatcgaatg aaaaacggtg agttcgaaaa cggcagtatg
      181 gttttacgtg cgaaaatcga catgaattca ccaaatatgc atatgcgtga ccctgtgatg
      241 tatagaatac ttaagcagga gcatcaccgc accgggaata aatggaacat ttatcctatg
      301 tatgattatg ctcacggaca aagcgattat atcgaaggta taacccatag catttgtaca
      361 ttggaatttg aggtgcatcg tccgttgtat gaatggtttc tcaatgagat agccaaagga
      421 gaatatcgtc cccgacaaat tgaatttgcc cggcttaact tgtcgtatac tgtgatgagt
      481 aaacgcaaat tacttgaatt ggtaaaaggt ggatatgtaa atggctggga cgatccaagg
      541 cttcctacca tcagtggtct gagacgcagg ggatatactc cggaatcgat ccgggttttt
      601 gcagaacgta ttggtgttgc taaaagagag aacgtaatcg atgtgggttt tctggagcat
      661 tgtattcgcg aagatctgaa taaaaaagcg cgacgtctga tggttgtttt agatcccatt
      721 aaagtggtta ttacaaattt tccagaggag gatgctgaag tactggaggc aattaacaac
      781 cctgaggacc ccgaagccgg aacccgcaat atatacttta aaagagaaat atatattgaa
      841 cgcgacgact tcatggaggt accgaataag gattttttca gactttcccc cggaaatgaa
      901 gtccggctca gatatgccta ttttctgaga tgtaacgagg tgctgaaaaa ccccgatggt
      961 agcattcact cacttatgtg cacatacgac cctatgagca aaggcggaaa atcaccagac
     1021 ggaagaaaag tgaaaagcac cattcattgg gtggctgcag aaaaagctat tgatacagaa
     1081 gtgagattat acgaccggct atttacagtt cccgaccctg ataatgttcc ggaaggtggt
     1141 gactttaaag attttctcaa tgaaaaatca ttaaaagtaa tcattgcaaa agcagaacct
     1201 tcgattgagc tggttgctat ttccgaaaaa ttgcaattcg aaagaaaagg ctacttctgt
     1261 tgtgattctg actccgaaaa gggagagcct gttttcaaca ggattgttga cttgaaagat
     1321 tcgtacccca aaacaataaa taaataaaat atgcaacgaa tagctttgtt tcccggatcg
     1381 ttcgaccctt ttaccgtcgg acacgaatcg attgcccgta gagcactgcc attattcgac
     1441 cgcgtaatta tcgggattgg taccaacgag aataaaaccg attttttccc acttgaaaaa
     1501 cggattcaat ggattaaaaa tttgtttgcc gatgaaccaa aagttgaagt gaaaacctat
     1561 aaaaggctta ccattgattt ctgtcgcgaa acgggagctt cttatctttt aaggggatta
     1621 cgtacttcgg cagattttga atttgagaga gctgttggtc aggtaaataa gctccttgat
     1681 ccggaaattg aaacggtttt tttacttaca aaacccgagc atagctttat ttcgtcctca
     1741 atcgtacgcg atgtttataa aaatggcggc gatataagta gttttattcc aaaaggaatg
     1801 aaattgaggt aaagggagtt ttcaggcaat cagattataa atacagttct ttttcgatgg
     1861 taagaattat attgtgaata atcatgatat ggatttcctg aattctgtcg gaatactcgt
     1921 tccatggtac ccgaatctca atgtcgcata acggaccgat tttgccccca tcttttccgg
     1981 taaggcctac tactttcatc cctttgcaat gggcggctct aactgcattt atgatgtttt
     2041 gagaattccc gctggtactg attgccagta aaatatcgcc aggcttacca attgcttcga
     2101 tatatctgga aaaaacttgg tcgaatccaa aatcgtttgc tgtgcaggta atatgcgcag
     2161 gatccgaaat tgcaacggct gccagaccgg gccggtcgtt tcggaatttt cccgaaagtt
     2221 cttcggcaaa gtgcatggca tcgctcatgc tgcctccgtt accacaactt ataattttac
     2281 cattattttg cagtacttcc gccattaatt tgcctgcttt tgcaattcgc tcaaagtttt
     2341 ccggattttc tgaaaattca gctaatagtg tttgtgcctg caaaaactga gttttaatta
     2401 cgtcgttagc cataataata tttttatcca aacttaagga aaaaatatgt tacctgcata
     2461 cacgctttca tatataaccg gatttattgt tgatgaagga gatgttacga atattttata
     2521 cttttggaat tattggtttt acagcaaggt aattgctata taggccattt taattatgat
     2581 gaatgaaacc atacttcaaa ggacttgata cgcttagagc tgttgcagct ttgatagtcg
     2641 tgattggtca tgttgaggag atcaaaaaca tattcggtat accaaacatc ttgcatagca
     2701 aaggctttca actcccagat ggtcatttgt cggtagtact tttttttgta ttaagtgggt
     2761 ttctgataac gtttttgcta attaaggaaa aacaaaattt tggtaaaata tcattacgcg
     2821 atttttatat ccgaagggtg ttcagaatta tgcccttgta ttttatggtt ctgtttcttt
     2881 cttttgtaat attcagacag gaatatcaaa caaatacgat aatactctgc gctaccattt
     2941 ttccaaatat tgcccacgct ttgtcaatcg ggtggcccac ttcgcctcag atatggtcaa
     3001 ttggtgtaga ggaacagttt taccttttat ggccattatt attaatactg atacccccca
     3061 aaagagtaac tatctatctg attttatttt ttgccggctt ttcgctgata catttattgc
     3121 ttgggtacgc caattttaat actgttcaaa atactggatt taatgatttt gtttacaagt
     3181 tcttttattt ttcaaaattt aacagtatgg ccttggggag catgttgggg tatatgtgcg
     3241 ccacaaacaa taagaatctg aggttcttat tcaataatta tgtagcatac gcttcggtgt
     3301 ttctttcatt ttttatgtgg ttttttcata tttcgtttca atatttcaac gatgagatct
     3361 actcaatggt ttttggagta atgataatta atttggctgc aaacaatagt tttaagttac
     3421 atattgatac aaaagtttca gtgtttttag gtaaaatttc atatggaata tacatgtatc
     3481 attggattat cattatgttg ctcatgaaac taataccgta tagtattttt aaaactaata
     3541 tttcatttaa cattgtcata tattcgttgg caattatatt aactatctgt gtatcatggg
     3601 tttcgtatgt tagtttcgaa aaatactttt tgaaacttaa ggaaagattt actcgaaata
     3661 ctgcgagtta gaaagtgaaa ggaataatat atattgcaga tcaatacagg tgctcaagta
     3721 attaacaaat tattaagctc tgtaaaattg caaaatgtta aatttgtcag ctgattaaat
     3781 aactaattct gaataatatg agaaaattac actatttgtt agtactagtc ttatcggtat
     3841 ttgctgtgaa tgcattttcg cagggactgg actacaacga aaaattgatt tttgacccgg
     3901 gagtaagggt tggcaagctt gataacggga tcacttatta tatcaaggca aatgctactc
     3961 ctcagaaaag agcagagctg attctggttg ttaatgcggg ctccgtgctt gaagacgaag
     4021 atcaacaagg acttgcccac ttttgtgagc atatggcctt taatggtaca cggaactatc
     4081 ccaaacatga attgattaat tatcttgaat cgctgggtat gaaatttgga cccgaggtta
     4141 atgcctatac atcgttcgac gaaaccgttt atgggataaa agtacccact gatagcgctg
     4201 aatacctcga caaaggtctg ttggttctct atgactgggc ttatcaagta agtttcgaag
     4261 atgatgagat cgaagcagag cgtggtgtta tccacgaaga atggcgtatg gggcagggtg
     4321 ctatggacag aatgcaacgc aaaatgttat caaccctatt ctatggttct aaatatgccg
     4381 aaaggctccc tataggtctg atgtcggtgg ttgattcttg cccgcatgat gtactgcgac
     4441 gtttctatag cgattggtac cgtcctgatt tacaggctgt tattttagtt ggtgatttca
     4501 acgcggatga tgttgaggct aaagtgaaac agctctttgg gaaaattcct gcaaaagatt
     4561 ctccccgttt acgtgaagtt atcgaaatac ctgatcacga acaaaccctt gtatcgattg
     4621 caactgaccc tgaatctcca attggtatgg tgtatgttgt gtataaacac ccgttgaaaa
     4681 gcactaccac tgttggtgat taccgtgagg atataattgc cgggctttat aatcaaatga
     4741 ttaacaaacg cttgcaagaa ctaacacttc tcgaaaaccc accttttgca cagggtgcaa
     4801 gcatgtacac atcatttctc gggcccaagt cggtatatat gagtattggt gttgcccaaa
     4861 acaatgatat tggcaaagcc cttgaagcgg ttactatcga aaatgtcaga gtacagcagt
     4921 ttggattcac cgccactgaa cttgaacgcg aaaaagcagc catgctcaag gaggttgaaa
     4981 aacaatacaa cgaacgtgca aaacaaaaat cggaagatct ggctcaggag taccaaagaa
     5041 actttctgcc tccttacgaa cccgttccgg gaattgaaaa ggaatttgaa ctttacaaac
     5101 agcttttgcc aacaattacc cttgacgaca ttaataattt tgcaaaaaca ctggtaacaa
     5161 aaaacaatac ggtaatctcg gttatgatgc ctgaaaaaga cggagttgtt attcccaccg
     5221 aagaacagat tctggaagtt tacaataaaa gcctgaatgc tcaggttagc ccatatattg
     5281 acaaagtatc agacaaacct cttcttgcag aacttccacc caaaggaaag gttgcaaaaa
     5341 aagccaaaaa taaagacctt ggatacgaag tatggacgct tagtaacgga gcaaaagttg
     5401 taattaaaca aaccgatttt aaagaggatg agattcgctt tcatgctcaa agttggggag
     5461 gcgcatcatt atacgaacag gatgccgata taagtgctga tattgcagcc gatgttgcaa
     5521 tagaaagcgg tctgggcgat tttgataaaa cagaacttga caaatatctt tcaggaaaaa
     5581 acgtatatct tactcccagt atctcagaat tatccgaaag cctgaatggt tcatgttcgg
     5641 ttaatgattt cgaaacattg cttcaaatga ttcatgtgtc tttcacaaaa ccaagagtta
     5701 ccgaaagcgc attcaactca tatattaata agcaaaaggg acttcttgca aatgctatgc
     5761 tcgatcctca atcggcatgg agcgacaccc ttcagacaac aagggccaac aatcaccctc
     5821 gtcgtagggc attaacccct gcaaatctgg acgaggccaa ttttaaaatg gcacaaaaaa
     5881 tagttaaaca acgatttggt gacccatcaa attttacttt ctattttgta ggtaatattg
     5941 atccgaaatc atcgaaagag ctcattgaaa aatacattgg aagcctgcca actgtaaccc
     6001 gtaacgaatc gtataaagat atgggaatac gggctcctca aggaaaagtt gaaaaaaccg
     6061 tttataaggg gaaagacaat aaatgtatgg ttgtaatgga ctataacgga acaatgaact
     6121 ataacgcaaa aaacaaaatt gaattgaatg cactgtgtag tattctttca accaaacttc
     6181 tcgaagaaat acgggaaaaa gaaagcggtg tttacactat tggcgcttat ccgcaggtac
     6241 agaagcatcc ggtgccacga tacgacatag ttattttctt cagctgcgat ccggcacgcc
     6301 tcgaagaact gaccaccaaa gttattgcag aaatcgacaa acttaaaaac aatggtcctg
     6361 cagaggcaga tcttttaaag tcgcaggaaa aactacgccg cgaatacgaa acaaatagtc
     6421 gcgaaaactc ttactggatt ggtaaacttt ccgaaattga atgtggagat ttaagtgtag
     6481 aagagtttaa aaatttcaac aactatgtta atcaaatcag tcaggaatcg ttgaaaacaa
     6541 ttgcaaatac ctattttaac tataacaact atattaaagt tgtattaaaa gccgagaaat
     6601 aacaatagca ttccagttca ataataggct gctcttgagc agcctatttg tttataatcg
     6661 aataattttg ccttgtaaag cttgacgtta gtgcattgtc gttaacatca atttgtttaa
     6721 atctatatca gatatttcgt agaaattaat gtcagctttt ttatcttagc atgattaata
     6781 ccgagaggaa gtaattcata tgccgcagca ccgcttacca ggttccgatt ttttaaggtt
     6841 taaagcactg aagtgcattg cggaagctgt acgaaatgac atcaaccccg ataggtttat
     6901 ttctgttgat aaaattggta aaaatcagca gtattataac ttaatggaga aagtgctgga
     6961 atataacact attaatcaaa agcttcggat tgaacaccaa acagagtata acaatttgtt
     7021 cagcaaggca aaaatgtatg ttttacatta tttgcaaata atcgacatgg cgatagaaag
     7081 gggtgagcaa ccgatttctg taagaaatta ctaccagctt gacgaatccg gtgggaagct
     7141 tccacagatt aacaacgaaa gcgaattggt aaaagaagcc acaaatttgt ttacaggcga
     7201 tgccaaacgt acctcggaag gcggaaaata ttttacaaat cctacgattg gattggtgaa
     7261 aatgtggttc gacaaattca tggaagccag ccagaaagaa aaaaacctga gcttcgttaa
     7321 aacaggagag gttgagaaca ttgctttatt gagaaaagaa atcaattgtt tcatcgatga
     7381 ggtatggtca gaagttgaga aaaactcagc tgcaagggga agggaggagc agctgaagat
     7441 atgtcatagc tttgggattg aatatgaaat gactcaggaa gatgttttaa ttggaaaaca
     7501 agaagatgtt tttaaagagc ccggcctttt tagcagtatg caaggcgatt gtgaggatag
     7561 gttaatgaaa gaagcatgtc atgaaagcaa gagcgagtcg ctgaatttac aattcgcttt
     7621 tgaatttccg aaataatatt gcaataagaa atattgtaat catagctgaa atcaaagtaa
     7681 acataatatc ggaactatta cttccgaaat cttcaaggtc tttttgaata gcccctttat
     7741 taatatagaa atatacaatt acagccatgg cattattgaa gaaatgtgca aggatggcag
     7801 gccacaacga acctgaatac ataacgagat acccgaaaaa tgctcccagc agaaaccgtg
     7861 gtataaaacc gaaaaattga aaatgaattg cactgaaaag tatagctgac aacaatacag
     7921 ccacatgttt attatgtgta agatcgtgta tgagcgactg aattattccg cggaacataa
     7981 gctcttcggc caatgcaggt aacagggcaa ccagtagtat atttatcaat aacgtatagg
     8041 tgctgttgcc agacagaata agctctgtca tcttttttgc catctgttca ctgtcctgta
     8101 aagaatggta taaacccgac atgcattcgg gaaaggtaat tgcttcgttc catttgataa
     8161 ccatattcat ccagggcagg gaaaagaaaa gaattacaac aggtaataca taccatgcaa
     8221 tgtggcaaac gctattcaac ttaagtcttt ctgccggctt cctgtatatc aaccaagcca
     8281 caattatggc cggtagtaaa aaactaaaaa atgaagaaaa aaactgtccc agtttaagta
     8341 ttcccagagc tccggcatca gggtttggtc cggttaaaga atttatatcg gttaccccgc
     8401 catgaatcag aacaattgca gttgtaattt gtgccagaat aaaccccagg gctataatca
     8461 gaatggtgag tgcaataacc cgaaagttct taagtatttg atcattgtgg gtcattttgc
     8521 tggccttatt attctcaaga tactgttacc gatagaaatt tcccgaatta catctcctgt
     8581 gatctccgga gctggaacgc cttgcccaaa ttcagccggg ccagagaaga cccttgcttc
     8641 atcccacagg ttgctttgaa tgaacaaatc aatcaacttc ctgccgcctt ccacgaccaa
     8701 agaggcgatg ttccgctgat acaacctttc ggttatctgg tttgcaatgt tcttgttcca
     8761 atcaataatc tctgtatcaa taccctttcg gatttctgaa ctaaacaata gggtaggggc
     8821 cttattatta aaaatattta attccttgct taaaactccc tcagcatcta ttgttatgcg
     8881 aagaggttgt ttcccaaacc agcttctggc ggtaagcata ggattatcga gaagaacggt
     8941 atttgctccg accataaagg ccgattcctc tgtccgccat ttgtgaacta aaatgcgaca
     9001 catttcattc gttatccata caggtttttc cgttgtgggc gatttccgga ccctatcaag
     9061 aaacccatcc gatgtctgag cccattttag gataatgtaa ggtcgctgtt gctgatggaa
     9121 tgtgaaaaaa cggcggttca gctccttatt ctcctcatcg agaattccaa agtttacttt
     9181 tatgcctgag gctttaagcc ttgcaatacc cgccccggca accaaaggat tggggtcgac
     9241 tgagccaact accacttccc caatgttatt gttgattatg gcctcggaac atggaggggt
     9301 ttttccataa tgagaacagg gttccagatt tacatacagt gtgctccctg ctagcattga
     9361 cttatctttg actgaattaa tggcattaat ttcggcatgt gacccaccat atcggtgatg
     9421 ataaccctcc cctataataa ttttgttttt aacaatcaca catccaacca taggatttgg
     9481 tgcaacaagg cccaagcctt gccgggcaag aataaggcat cgtttcatgt atgtctcgta
     9541 tcccattact tttgcattac agcaaaaata catttaaaaa tgaaagttat gcttaatcat
     9601 ggattctaat gcaaaaatta aaacaagtgt atcagtaaat aatataaaaa tacaccttgg
     9661 ctttgtaaga acagtaattt attttaattt tggataatta aacctcccac aatgaatgta
     9721 ttgctggtaa tactgggatt actgatgctg gttgttggca gcgagctttt ggtcagagga
     9781 ggtgtttcga tagcaaagcg cgttaaaatt tccccgctaa tcattggatt gacaattgtt
     9841 tcgatgggca cctctacccc tgagttggtt gttagtgtaa aggccgcttt gcagggttat
     9901 cccgatatgg cgatgggaaa tgttctagga tcgaacattg taaatattag ctttattctt
     9961 ggccttacgg ctgtgatttt agccatacct gtaaatcggg aaacagctgt tttttcatgg
    10021 ccggtcttat tgttggcaac ggttctcctg tttggtttct catgcgattt taagttggcc
    10081 cggtgggaag gcattgtgtt tgttgcactg caagtgctat ttattgttgc actgtatttc
    10141 aggtcaaaaa gaaaaaagca acaggatgaa gaagattcgg gtaaaaccta tagcattgtt
    10201 gtatcaatac taatgcttat tgcaagtata gccggattgt cgtttggggc ggagtttctg
    10261 gttaagggtg ccgttggcct ggcaacgcat atgggggttg atgaacgcat tatttccctg
    10321 actatcgttg ccattggtac cagtatgccc gagctgatta cttccgttgt cgcagccatg
    10381 agaaagcaga tggatatttc tattggaaac ctgattgggt caaatatctt caatattttt
    10441 acaatattag gtgtttcttc ggcaataact cccataactg taaatccaca gtcactttat
    10501 gttgatatga cttgggcatt tggattggta attctgctgg ggttgtgctt catccctctg
    10561 cgaaattctg ttgttaaacg ttggaaagga ttgcttctgg ttgttgccta tatggcttac
    10621 ttcttttata tcttttaaag aaagaagtaa aaactatttc gacctactta taagctgaag
    10681 ttttccccca ctcatgcagc cagtacattg cttcggtttt aatatgcgag tggtttgtgc
    10741 ctccgctttt aaaaagcttc ccgggaattt tcccgtacaa gatgagattt ttagcaccat
    10801 ccttaatacc cttaagtatt tcgaaagcgt tcagattgaa tgcacgcccg gctttttgca
    10861 tccacgaaac gatgtgcccg attacaggat agtcttttct gatgaaatgc attcttattt
    10921 catcttctgc tctgagaatc aattttttaa gatgtttttc cggttttcct gacatgttaa
    10981 taacacggtc gcttatgtct ttatcggcaa gttccataag ccaggcttct tcatcggtta
    11041 tatatttttc atcgatggct ttgcgataaa gcaatgtgcc ggggtaaggg caaggtattg
    11101 cgaaattcgc actttttagg ttcaatgctt tacaccaagt aatgttttcc tgcatcgatt
    11161 cggtgccgtc gctggggtat ccaaataata actgtgccga tgccagatga aagccgttgt
    11221 tctgaattac ttgaattgca tttataatgg tatcagcatc ctgtttttta ttcatatcgt
    11281 gcaggctggg attgtaaaac gactcaatcc caatagcgat actgcaaaag ccggcttgct
    11341 tcatcctcga aatcatctca tcatcaaccg aattggcacg aagcccgttt ccgatattga
    11401 gaatatactt cttatcactg gcaattaatg catcacaaaa ctccattacc catttgcgat
    11461 cgatattaaa agtttcgtct tcgataataa aattaatggt gttatatttt tcttctagga
    11521 atgccatgtg ttttagcgta taatctacag aaagcccgac aacctttctg ccgaaattcc
    11581 gataacagaa attgcactca taagggcaac cccttttcga aaacagcatt accggacgac
    11641 tagccttatg tctgtcgagg ttgaattttt taattctttc ttcggaaaca ctgttttttt
    11701 tgtttttata aatgtaggaa tttgaaatgt atttctccat gtcgatgagg tgatatgccg
    11761 gaagtggcat atcttcgaga ttgagcatcc gaggacggtc ggcagttttt atcacattgc
    11821 cggttttgtc gcgaaatatg ataccgggaa cagtattcaa atcccctttt ccggtaagag
    11881 cagctacaat atcgataata accggttctc cttcacccaa tgcaattaca tcgaatttgg
    11941 tgtgttttac cattacatcc ggcgagccgg aacatgctgt gccgccggca attaagggaa
    12001 cattggggta atcttttttg agatattctg ccagaaattt catgtagtaa tacacagtag
    12061 ccatgcctcc tgtgccaatt acatcgaact ggtgttgggc tatttcatgt tttaagtcgg
    12121 taaatcccag ccgcagcatg ttcaagtcga gaatttcgac tgcggcaccc gctttttcta
    12181 gtacagcagc cagcgatagt aatccaaaag gggggtacag cggtttttta tttagcctta
    12241 ccggaggata tatcaacagc acttttgtgc ccagaagtat gttttcattt ctgaaaacgt
    12301 caaaatttgt tgataataca tttgcttctg ccattacaat caatttcggt tctttgtgac
    12361 gaagcaaagt taataaatca tggcattgat tataattatc cctgtttttc tgtgataata
    12421 tgttgggaag agatatcttg cagacacccg atttcggcat ttgaataatt ttaactactt
    12481 tcgtgttgaa tgaaccaaac ctttaaacag gtgaatatgt ggattaaacg gttgaatcaa
    12541 agtctgttga gtttaattaa ggttttttta tattcaaaaa tccccgaggt gtttaatcgt
    12601 gcagtagtaa atgaagcggt tattttgggt aatggccctt cgctttcggg aatgatatcg
    12661 gaaaacgcag aatttctgaa agggaaagag ctttttgcgg ttaactattt tgccctgagc
    12721 gatgattatc atacgctgcg accccgaaac tatgtaatga ttgattttac tttttactac
    12781 aatactattg gggatgaaga gtcaattctc aaaagggata aactttggca acggattgct
    12841 tcctttacta cctgggagat gaatctttat ttgcctttca atgcaaaaaa acattcttac
    12901 tggaaaaagt ttgtttgtga aaacaagaac attagggtta aatacattaa tgttactgcc
    12961 gttgaggggt taagaggcct tcgcaattac tttttcagca agatgcttgg catgccccga
    13021 ccacataatg taattatacc gtcgctgatg attgctgtta aaatggggtt taaaaccatt
    13081 tacctgtggg gggtcgatca ttcctggctg gccaatgtaa gtgttgacga aaataatgta
    13141 gtgtttgtaa acaataaaca cttttacgat ttcaacactt cgaaaccggg gttgtatatt
    13201 ctaaaaaata ctccggctgc aaggatgcac gaaatgctgt attcgtggat gtgtactttc
    13261 gaaagctatt ttatcataaa cgattatgct tcgaaatata atgtgtcgat tataaatcaa
    13321 actccgaatt ctttgattga cgcatttgtg aaaaaacgta ttgaataaaa ttatttctta
    13381 acaaaaacag caatccctgc cttgaagtaa ttttttattg cattccgtat atcgggagat
    13441 atgtttagaa aatatacggg acccacaaaa aacactgttg taatacctgc tttaataatg
    13501 atgtcgataa taaaattagg tgcatcaggg atgaaatgca gaattataaa aacaaccaat
    13561 ccaatgatta aggcatagag gtgccgaaga ttatagggtt gcaaacccag tttcgactta
    13621 ataaagaaga aacggattac agcaaataca atgaatgagc tcatcgatgc cagagcagct
    13681 ccggtcaatc ccattcgggg tatgaaaaac atattgctgg caattaccat tgcaataaaa
    13741 acaagaagca ggtaagcgta aagccggtag tatttcgagt actgtattat ttcgtagctt
    13801 gctccactta acatggtaag cacttgaagt atcccaatat agaataccac cattttaccc
    13861 tcggagtaat agcggggaat tatctcaaat gcactatcaa tattactcca tataagtaga
    13921 aaaagatata tggaaattat aaattgtgta agcgtacttt tagtatatat ctcgccaatg
    13981 agagcggtat cgtttttttt gaatgcttcc gatataaggg gagaagctat tcgtttgagc
    14041 gatctcccag caattaggat aatggttcca aaataaaaca ttgtagaata cacacccgct
    14101 ccggcaagcc cgcaataatg atttaccata taaatatcga tattcattac tgcaatcccc
    14161 gaaaatccaa tgattaagcc ataaaacgac acccatatca gctcatttct catgggtttt
    14221 gtgatatacc ctttgtctgt tttaaggtta tattctcctt ttaaacgcaa atatgccagc
    14281 aatccgatag tgggtacgct gtaggcaagg aaatagaaca atacaaatcc cgcaaaatcg
    14341 aaaacggaga agtaataaag cacaatgcct gcagtgatta aaatgcgcat cagaaattct
    14401 ttatagaata tgccaatcga tgcattaaaa agaacaatac aatagctgtc gtaaatcagg
    14461 tagaatatag ttatcaatat catcggtggt aaataccaga tataatcaat aaagagaggc
    14521 gatttttcga tattactttc gatgagatag ggttttaaaa ataaaaacag aacaaaagtc
    14581 agtgcaaaac caagaatact gacaagggtc ataaccttta caaatccatg gtgagaatgt
    14641 tctttgtccc tgaaaaacgg gaacagcttg attatggaat tcgaaaatcc aagtgttccg
    14701 agctgtgcaa tgattgcaga gactgatacc agaaggttgg tcaaaccaat ttgttctttg
    14761 ctgaatataa ggggcatcat taatcccaca ttaataaagc ccagtaaggt gccaatgtag
    14821 gagtaggccg agcctttaac cgattgcttt actattatac ccattacatt gaattaaatg
    14881 ttgcctggaa aaaaatatgt gtaaaaataa ctgtttattc gtgaaactca aactcaatca
    14941 aaatgagaat attccttccc tgaaacaaag aaaacaggtg ggattgacct cttgaattgg
    15001 ctgtgaggat aaattattga aacagcagtt tttgtttcga tggcaaattg tttggatcaa
    15061 tctgaaatac accgtaaata gaggaagatt ctttcagcac tttcagaaac ttttctgcga
    15121 acacaatact gtcggctccc ttgatgatta taaagtaatc gatgttcttc agttcttcaa
    15181 ttaaaaagcc ctcctgcgat ttattgctta ttaacgaaat gctcaggttt tcatcgtttt
    15241 catcgcactc gaataagcta aatgtttgct ctattggtaa tttctcatga aaaacacata
    15301 aattttcaca acgatgaaag tccagttcgg tcattgtttt aagaatgtaa acaagccgga
    15361 tatctgatac tgtagcagaa aggccaaaca aaataaagtt gtattcaggc gagaacttca
    15421 gggtttttct tttcatttca acaagtttta tctgctttca gaagcaaacg aactgcaatt
    15481 ctgcttgctt cctgctctgc ttctttcttt gtccatgcct tcccttcggc tatgaaaaca
    15541 ttattaacaa gtatagcaca ggaataatga atttcttttt cacagtcttc tatgttttcg
    15601 taagaatcga aaacgatact atgtcgtctg cgctgcattt cctgaagcaa cagcgactta
    15661 taatcctcgt tgtctttatt cagggttgta atatttatat gggttttaat aattgcactt
    15721 atatagctat ggcagcgcag atatccctga tcgaaatata tggctccaat aagcgcctct
    15781 aaggcatttc cggcaatgtg tcctgaattt ttatcgtttt cgcttttact tcgataaatt
    15841 aaaaaatcgg tgattcctat tttgtgggcg agataatcga gtgattttcg tttcacaatc
    15901 tgcgagcgca tcatggtaag atttccttcg tcagcttcgg ggaaggcggt aaacaaaata
    15961 tcagctacta ctgcatccag aatggcatct ccaacaaact cgagtctttc gttatttatc
    16021 tgaacgccgt cagggctgtt taaacttgcc gaaatgtgcc ggaaagcagt ttgataaatt
    16081 actatatctt taggataaaa acccagcaac ttgtaaagct ggtatgaata cgcttttgtt
    16141 ttacgattaa aaaacgacga aaatctggtt tttaaaaaca agagttatgc ttttttaaag
    16201 ataatcgaag tattatggcc tccaaaacca aaagtgtttg ttaacccgta ttcaatatct
    16261 cttttcacag atttgtgaag cgtaagattg aatctctcat caatttcggg atctttttca
    16321 tgatggttga tggtaggcgg aacaatgttg tttttaacgg caagtatggt aaacattgct
    16381 tctatagcac cagctgctcc tagtaagtga cctgtcatcg acttggtgga gctgatattc
    16441 aatttgaatg catggtcctg aaatactcct tttatagctt ttagttctgc aatgtcgccg
    16501 agcggagtag cggttccatg gacgtttata tagtcgatgc aactgggttc tatgcctgcg
    16561 tctttcaatg catttttcat taccagcgaa gcacccagac cctccggatg gggtgctgtc
    16621 atatggtaag catctgccga aaggcctacc cctgaaagtt cagagtagat ttttgctcct
    16681 ctatttattg catgttcata ttcttcgaga ataagggcag cccctccctc ccctaaaaca
    16741 aacccatcgc gtgtggcaca aaatggtctt gaggcggtta gataatcatc gttacgtgtc
    16801 gaaatggcct gcaaggcatt gaagccccca ataccaatct cgtttactgc agcctctgat
    16861 cctccggtaa caataacatc agccattccc aaacgtatca ggttatagga atctattatt
    16921 gcattgcttg atgaagcgca ggccgaaaca gtgccataat ttggacccat aaatccgaat
    16981 ttaatggaaa taagtccaga tgcaatgtct ataataatcc tgggtatgaa aaatggacta
    17041 aacctgggga tgcagttccc atcgaaaaaa cctttcagtt cctgaattaa tccttcaaca
    17101 cctccgattc ccgaagccca tataactccg gccctggtct tatctatttt atcaagatca
    17161 agtccggagt cattgactgc ttctgttgaa ctaatcaatg catattgcgt gaaaaaatca
    17221 tattttcgcg attcctttct gtcgaaataa ttcgctggat cataattttt tacttcacag
    17281 ccaaatttag ttttaaagtt ggttgtgtca aaatgggtaa ccagatttgc accgctaacg
    17341 ccgttaatga tgttattcca ggtttcgctt acattgttcc ctagtgggct aattgtgccc
    17401 aatccggtta caacaaccct ctttaactcc atacattgat tggtttaggg aaatgatgtt
    17461 aattggtatt ttcttcgata tatttgatgg cagcgcctac tgttgatatt ttttcagctt
    17521 cttcatcagg tatcgaaatt tcaaattctt tctcaaattc cataattaat tccaccgtgt
    17581 cgagtgagtc ggcacccaag tcgttagtaa aactggcttc cggtgtaact tcagcttcat
    17641 caactcctag tttgtcaacg atgatgcttt ttactcttga tgcaatgtct gacataattt
    17701 tttaatttag ttaaacttta atttcaaaat ttaggctaca aataaacaaa ataaagtaat
    17761 gataggcaaa aatataagat taaaaataat aacaaaactt gttaacaggt ttattttcag
    17821 taagtttgtg agttattaat ttatttaaga tgaagaaaat tgccattttt gcatcaggaa
    17881 caggttcaaa tgcgttgaat attatcggtt attttaggca aaacccgaca atttcggtaa
    17941 cagatttgct ctgcaataaa aaagatgctc ctgtaataga gaaggcgaaa aatgccggag
    18001 tacgaacatt tttatttggg aaagaggagt tctacaattc cgatgtagtc tacgactatc
    18061 tggtagggca taatatcgat gttattgttc ttgccggttt tttgtggctg gttcccgaaa
    18121 aaattgtcaa tgcatttcca taccgtattt tgaatattca tccggcattg cttcctgcct
    18181 ttggaggtaa gggcatgtat ggcagcaaag tgcatgcagc tgtgctaaaa agcggagacc
    18241 ctgagtcagg aattaccatt cattttgtta atgtacatta cgatgccgga agcattattc
    18301 accagtcgaa atgcagcgtg aatgataccg ataccccaga gagccttgct gcaaaagttc
    18361 atgagctcga acaccgatac tatccgggag ttattgaaca agtggcactg ggattgcttc
    18421 cctgagaaat gatgcggtta caaagaaatt aatagatagg ttgaattatt cctttataca
    18481 gtgttcatca tgttccacag gctctgcgat ggtttgtttt ttgattccga atacgcggtt
    18541 ataaaataca atcataataa gtaccgaaat agtgattgag gcatcagcga tattgaaaat
    18601 aaatggaaac aacgatgtgt cgccaatttt tgccgtgatg taaaacatgt caactacttt
    18661 tccggttaaa aaacctgcat accctccttc aggcggaaat atagatgcaa cctgcaatgt
    18721 tgtactttcg ttgaaaataa gaccatatac tgcgcaatca ataagattcc ctgtagcacc
    18781 cgctatcacc attgaaatac aaataataaa aagcagtttt gcctgagttt ttatcagttt
    18841 aactatatac caaaccaggc cgctaattgc aatcattctg aaaatactta ggccgagttt
    18901 tccccatttc ccccagaatt ccatgccaaa agccatgccc atattttcga taaattgtat
    18961 gctaaaaaaa ccgtctatta cactgatatc ctcaccaatc gtcatatgag tttttaccca
    19021 tattttaatt atctgatcga tgagtaaaat agcaaagatt aatacgatag aaattaatgt
    19081 ggctcgtttt ttcacttaat cgggtttact gaaacgggtt acatctgcta caaatgtgcc
    19141 tgtgattttt tttcaggtaa attacttctg gttgtttttt gcatcgatac acaaggtggc
    19201 atgtggaaca gcgcgtaaac gttcttttga aataagtttt cccgtttcgc gacaaatccc
    19261 gtaagtccca ttttcgatgc gcatcagagc ggcctgtaag tgttggataa atttaagctg
    19321 tcgttgagca agtcgtcctg cttcttcttt actaagcacg gcagcacctt cttcgagtac
    19381 tttaaaagta ggagaagtgt ctgctgtatc gttggtatct ccgtgattca aagcattttt
    19441 ataaaggtca taatcgagtt tggctttttc gagcttttcg agaatgattt ccttaaactc
    19501 ttccaattcc gctttgctgt atcgcttctt ttcttctgcc atggtattgt tattttcgtt
    19561 atttcccaat gcgttctacc cgtataagag tttcaaaatt atctatttct acgattttcg
    19621 aagaattatg ttccattttg ttcaaaatat ttatcgaatg tgccaaagtt tgagcacaaa
    19681 tgtaatcatt aaagttaata acactatcgc ttaattcgga atgatgttga acaaagatgt
    19741 ttatcttatc ggttacttca aatccgctat cttttctcag attctgaatt ctgttaacca
    19801 actcgcgtgc aattccctct tgtttcaact gaggggtaac tgtaatatcg agagccacgg
    19861 tcagggtgcc ttcgtttgca accaaccagc cctgaatgtc ttccgaaaga atttcaacat
    19921 cagccctttc tatacgaatc tctttccctt cgtgttgaat atttataaag ccgtcttttt
    19981 cgaatcggtt tatatcttcc tgactgaacg actgaattat ttcggaaata ggcttcagta
    20041 gttttccaaa tttagggcct aaagttttga aattcggttt gattttcttt ataagtaagt
    20101 ttgatgcgtc tgtcagatat tttatttctt taatattggt ttcagtcagg atgattgatt
    20161 ccacaagtcg gagttgttct gcaaagctat cggttaaaac aggaaccatt atctgctgca
    20221 gtggctgccg aacttttata tttacccttc gccgcaggct gagaatcatc gatgaaatat
    20281 cctgagccat ctgcatacgt tcttccagat acttgtcgat gcaatttgca tctgcaacag
    20341 gaaaagaaca aaggtgtacc gaatcagcag attctttccc ggttgtttga ttcaggtcgc
    20401 ggtatacacg gtcggtgata aatggtgcaa tcggtgatgc cagcttcgaa atcgttataa
    20461 gacaatgata tagggtttga taagccgata ttttgtcttc gctaaaatct ccaccccagt
    20521 atcgtttccg cccaaggcga acataccaat tgctaagatg atctgccaca aaagtctgaa
    20581 tggctctccc cgcccgggtc ggttcatagt tttcgtagtg ctctctaact tcaattatca
    20641 gcgagtttag cagcgatatt atccacctgt cgagttcaga gcgctcgctt acaggaattt
    20701 ccttttcaga agcagtgtac ttatcgacat tggcatagag agcaaagaaa ctataggtgt
    20761 tgtagtaggt tccaaaaaac ttacgcttga cttcgtcaat accagcatat atatttttcc
    20821 cggtttcagg atctttttca acatcaaatt tcagattatc ccaaggctgg gcattcgtta
    20881 tcatatacca gcgtaacgca tcggctccat gttgttcaat cacttcgaag gggtcaacgg
    20941 cattcccaag ccgcttcgac attttgtttc caaatttatc gagaaccaat ccgttcgaga
    21001 tgatgttttt aaatgaaaca ctgtcgctaa tcattgtgga aatggcatgc agggtaaaaa
    21061 accaaccccg tgtttggtca acaccttcag caatgaaatc ggcaggaaac aattcgttaa
    21121 atctttcgct gttttcgaaa ggataatgcc attgagcata aggcattgcc ccgctgtcga
    21181 accaaacgtc aatcaggtca ggttcgcggt acatgggttt tcccgattcg gaaactaaaa
    21241 ccactttgtc gacaaatggc cggtgaaggt cgattctggc atagttttca tcagaaaaaa
    21301 ttcctggtat aaaaccctca aaagggttct ctttcatata accagcctga atagatgctt
    21361 ttattccggt gcaaagttct tcaacagagc caatacatat acgctcactc ccatcggaag
    21421 aggcccagat aggcaatggg gtaccccaga agcgcgaacg actcagattc cagtcaacca
    21481 gattttcaag ccatttcccg aaacgacctt ctcctgtgga agcaggtttc cagttaatgg
    21541 ttttattgag ttcaattagg cggtccttaa aagccgtcgt tctgatgaac caagagtcaa
    21601 gcggataata gagtattggt ttgtcggttc tccagcaatg cggatagttg tgaatgtgtt
    21661 tttcaatgcg aaaagcctta ttgttctgct taagcatcac cgaaatttcg atatcagccg
    21721 aaaccgtagt ttcattggca ttgtcgtcgt attcattttt cacatacctg cctgcaaaag
    21781 tactgtagct tgctgtattc acatattctt ttaaaaagtc ggggtgcatt tcttcaattc
    21841 taaagaactt gccctgtttg tctaccagcg cctgtaggtt tctgtctttg tcttcgataa
    21901 gaagtgctgg tatacctgcg gcttttgcca ccctcaggtc gtcagcacca aatgtgggcg
    21961 cgatatgtac aattccagtg ccgtcatcgg tggtaacata atcgcccgaa atcaccctga
    22021 atgcattcgt catgggtttc acccaatcga taagttgacg atacgcaata ccaatcagtt
    22081 gtgaacctgg gaatgtatta tttatccggt atggaattac tttatcaccc tgtttgaagg
    22141 tactgaaatc aagctgtaat ccttccggtt taaaatatgc agaaatccgg tcgctgcata
    22201 acactacttt tattctgttc ccggtatatg gattaaaggt ttcaacaaga gaatactcaa
    22261 tcgattcacc aacagccaga gctgtgttgg agggtaaagt ccaaggcgtg gttgtccatg
    22321 ccagaatgct aatctcatcg tcaggggtgt gaaacaggaa tgatgacttt tcattacgga
    22381 ttacttgaaa catactcaca caagtggtat cctttacatc gcgataacac ccgggctgat
    22441 tcagctcatg tgtgctaagc cccgttcctg ctgcaggtga atagggttga atggtatatc
    22501 ctttatatag atatcctttt tcgtacagtt ttttcagtag gtaccaaagg gtttcgatat
    22561 agcggttatc gtaagtaata tatgggttat ccatatcgac ccagtatccg atttcgcgtg
    22621 taagttcctc ccactcccgt gtgtatttca tcacttcggt gcggcaggtt ttattgtagt
    22681 cttccacact aattttttta ccaatatctt ctttggttat tcccaatgtt ttttccacac
    22741 tcagctcaac aggcagacca tgggtatccc agcctgcttt acgctttacc ttatatccat
    22801 caagggtttt atagcggcag aaaatatctt ttatagtcct cgccataaca tgatgtatcc
    22861 cgggtttgcc atttgcagac ggaggcccct cgaaaaaaat aaattcttta cagttttgcc
    22921 gtgttgttat gctttttgca aaggtgtttt ctttgtccca caattcgtta atgtctctgt
    22981 ttatctgagg cagatcaagg gctttatatt ccggaaattt ttttgacatt aaaaattttg
    23041 tttagtttta taaagattgc aaatatagag tataactgaa aatttgcaaa atgatattta
    23101 ggcttgttct atcccccgac aataattact attcaaataa atgcacttac tggaacaccg
    23161 attgagctga tttatttctt aagaatgata gtaagtgtct ctccggccaa caactgatgg
    23221 ttgtcgggca gcttattcca tttcagaatt tcatcaactg tgcatccata ggtaatcgca
    23281 attcggtgta gcgattcccc cttttttaca acatgctgca cgggaacctg ggttgaagat
    23341 gtaccagaag taactaccat tgtattcctg aggttttttg ttgactgaga gagagagtcg
    23401 gaaacaattt ccaggtattt cgaaatgtat tcatggggta aaaccagtgc atattcctta
    23461 ccatcggaag gtataaagtt gcggtaatag gtcggattta acaatagtat attttccgca
    23521 gggaattgca tttcgttggc aaggttaaca aggtttaaag gtccaaaaac atgaatcgta
    23581 tcaacgtcaa aaaagtcgaa atcggatttc aatggaataa tttgatgttc gcgactgaaa
    23641 ttcatcaagt aacacattgc aatgaacgct ggtacgtaat tttgcatttg ctctgtcata
    23701 tattgtctta tttgccaata atcgttgctc cccccacgaa ttattgcttt tttaaccatg
    23761 cctggacctc cgttgtaggc ggcaagaacc agcaaccagt ctccaaatgt ccggtacagg
    23821 tattcaagat agcggcaggc tgcatcggta gaccgatacg gatcgcgtct ttcgtcaacg
    23881 taggaactta cctgtaagtc gaataaatca gcagtaggtt tcatgaactg ccataaaccg
    23941 acagcaccgg aaggggatac agcattggga tcgagggctg attctactga agcgagatat
    24001 tttaattcca gcggcaaatt gtattttgca agatactctt cgaaaagagg gaaataatac
    24061 tccgataaac cggctatgcg attaagctta tccctattcg gaagggtgta ggcattaata
    24121 tatttctgaa catattcatt gtattgcaaa ggaatcaggg ttgtccttcc aaggacttca
    24181 attcgaagcc ggtaaacttc aaagggataa acaagactat cggaaaagag aacttcgtcg
    24241 tggtgtatga aacttttttc cggctttact gaattaagca tgctgtcgat atgcatagtc
    24301 agactttttt tacccttttc gccgccggta atactatggg caggtagatt attgaaaagg
    24361 aacaataatg atataaatat cccccaatat tgaagaggct ttttcatgtt gacaatatct
    24421 taattatcaa gctaaaattc aatattttaa gattgttttt ttaacttgta aatgttaatt
    24481 attaagggtt ttaattattt gtccccacat tttgtatgta tatcttagca agaattgaaa
    24541 aatttttagg ccaatgaaaa agaaaacact gataattatt gcgggaatag tgcttgttgt
    24601 agccattggc gcaattgtgt attttgtttt ttttagcaga aaagacgtag ttgtttccga
    24661 atcgataaag gctattccag cgaatgcttg cattgttatt caaatacgtg atgtaaataa
    24721 aacttacgag ggtttgaagg aaaagagcaa aatttggaat gaactctcgg ttttacctgt
    24781 tatttcgcgg tgcgataact ttttgtcaag aatctctaaa atgagaggca ctgaaaaaat
    24841 tgttgaagac cttctttcta ataattcgct gtttatttct gtgcaccctg ttggtaaatc
    24901 aaaatacgag atttttcatg caatcgccat tccgagtgat ataaaccccg aagaccttat
    24961 tgcattgttc aaaaaagaat atgccgattc agcagtatgt agcgagcgca gcttcgaaaa
    25021 tgtaactttg tatgaattga aatacaatat cgggaaacgc agtctttgtt ttgccatagc
    25081 aggggggcat cttatttgga gttattcacc tttacttgtt gaggaatcgg taaatcaaat
    25141 gaaaggcaag aaaaaaagtc ttgccgacaa cgaagatttt accacgattt ataaaagtgc
    25201 cgggcagaaa gagctggcca atatatatct gcaaattcct tctttacctt tacttttagg
    25261 tactcaactt aacgcagacg catcgttgct gaaggatgca gttgttggtt tcgcaggatg
    25321 gacagagctt gatttgggaa tcgatgatac ccggtttaca tttagtggtt ttaccgctgt
    25381 gaacgattcg ttgttgggct tcgtaaatgt gctgcaatcg cagaaacccg ctccgatgac
    25441 catagaaacg gtgctaccgg caaacacata tttttacatg caaatggcat ttaccgatgc
    25501 caatgcatat gccgataacc tgataaagtt cctgaagttt actgagcaaa acatcaatta
    25561 cgaaaagtta tgtaaacagg ctgaaaatga atatgctctc aacttaggtg aattgatata
    25621 cccacttatc aatcgggaag ttggtttagc aatggttgaa agtagcaacg gggaggacga
    25681 gatatactca tatcttatta ttaatactaa aagccgcagc attgcagaaa atgagcttga
    25741 aagaatttgt gaaaaaatat caggatccgc aaaatataaa tctgttataa atattgatga
    25801 agcagtagaa tttaatgtgt atcgtatgga tttccccgat attggggatg ttttgtttgg
    25861 cccggtgttt agtgggctca actcgaatta tgctgcattt tacgataatt acctgatttt
    25921 tggtaatagt gtagctgcca tcgatgagtt tttgcgcaag gctttactga ataaaacaat
    25981 ggctaccgac ctgaaacacg cagggtttat gtcgggtttt tcgaaaaact cgaatatgtt
    26041 tgtatatgtt aaccctatgc gtgctaaaaa tcatatctgc aaaattctaa cagaagaggc
    26101 attgcatata ttccagaatt catttcaatc gatctcccgt ataagcgata tcggattaca
    26161 atggagtgtt tctgagggta tgctctacaa ctacgcaatg atccagtttg atcctgagca
    26221 gagagaagag cccaaaacca tatggacaag caaactcgat tccattgttc tgaataaacc
    26281 ggtgattgtg gtcaatcact tgaatcaatc aaaagagatt attgttcagg accgcaaaaa
    26341 cattttacat cttattagcg aaagtggtcg tgaaatatgg aagataccgg ttgatggccg
    26401 cattaatagc gaagtatatc aggtagatgc atttaataat ggcaaacttc aatacttgtt
    26461 ttccaccgag acgcagatac atcttatcga cagactggga aatgaatgtg accggtttcc
    26521 ggtgaatttg cgtgaaaaat ctaatacggg aatatcgctc ttcgattatg aaaataacag
    26581 gtcataccgc attgcggttg cctgtaacga caagaaggta tatctctacg acattgaagg
    26641 gaaattggta aaaggttgga tgtttgaaac ttcagataat cccgtgctgc aatcgcccca
    26701 gcatttcagg gtggggtcgg cagattacat tgttttcaac gatctttata aattgtatgt
    26761 tgtcgatcgc aaaggccaaa ctcaggctgc tccgtcaaca ggttttctgt tttccgaaaa
    26821 caacgatgtg ttttttgatg ccacggggca ggaacgattt attgcctcag cgaaaaacgg
    26881 aacagtgtat tttatgtatt tgaatggaaa aaccgattcg atttcgtttg actcatatag
    26941 cgaaaagcac ttttttaatt actacgatat tgacaacgat ggaaaaaaag actttatctt
    27001 cattgacggc gggagtattg atgtgtataa agccgataaa aagaagctct tttctgtaaa
    27061 tcttaattct gatatcaatt accctccgta tttttataaa ttctcacgca accaaattaa
    27121 gattggtgta gtctgtaaag ataaagagca aatatatctg ctgaacgatg atggtacttt
    27181 attcgaggga tttccgttgc gtggtatttc accattcagc atagggtatt taaacaacaa
    27241 cagcggcagt tttaatttgg taaccggtgg gaaagacaat tcgctgtaca attatgaaat
    27301 taaagaatca tcggaataat tcgaattctg tagtatcttt acataaaaat gcaattcatt
    27361 atgaaacgct ttctgataag ttcttttatt gtggggtttt taataatgct ttttaattcc
    27421 tgtataaaag acaattttga ttttgataaa tgggatggtg aagtggaatg gaccccatcg
    27481 tttgtagcac ctgtagcatg gggcgatttg agtatacttg aagctatcga agcctacgat
    27541 agcacgatta acctgattgc gaacaccgac ggatatgtgt cgttgctcta ttatactaac
    27601 gtgagctcaa gagatatttc ggagcttatt gtaataccag atcaggcata ttttggggtt
    27661 gttaacggaa cagatatcga tttttcggag ttcgatcaga atggcgaaat actatcggga
    27721 actgtaaatc taaagatggg gtttacaatg ttcaacgaag aatgcgaaat agataccatt
    27781 tatgtaaaat cagcgattct gaactctctg gttcaatcta cttataagca tacactgcag
    27841 ttgaaattta cgtttccaac cattgttaaa aacggtagcc ccttgcaaat agagtacttt
    27901 tatgcaccag ttggcggcat tgatgcaaac aacgatatta gccttcaggg atatcatatc
    27961 gatctgactc agactgcgct tggatacaac gaagttcctg ttgttatgag ctatatcttt
    28021 tatcattccg gaacccctga taactcagga aacatcatat tcaatcacaa ttttgaagat
    28081 ttggaatatg aattcatgag cggatacttt ggttataatt caattatttt tgagaccgat
    28141 tcggtaaata ttggcatatt taaaaacaaa aacctgaatg ttgatgagta tttgtttcag
    28201 gatcctaaat ttaaggttcg ctatcataat tcctatggaa ttcccacaaa ctttttcttt
    28261 acagaaatgt atgcacgttc gaaaattaat gaccagaatt ataatatcat agattacgaa
    28321 aacggtctgc cgctagattc gttgcaccca tactttgtaa attttccgaa tattccatgg
    28381 tttatgcgag acgacagcat agtgctaaat cgtttcaaca gcaacattgc ccaggtaata
    28441 agtattcagc ccaaatggat tcagtttgtt gcgcatgcac ataccaatcc gaacggaaca
    28501 tcgcatacca attttatcac tgatcaaagt gttcttgtag ccgatgtgga agttgaactt
    28561 cctctgtggg gatatatcga caactttcat gctgccgata catccgactt cgacttcagc
    28621 gaaatgtata gcactgccca ggcgataaaa cgacttgcaa tcaggcttga tatgactaac
    28681 ggatttccag ttgaagggta cgggcaggtt tactttatgg atgagaacta tacaattctc
    28741 gactccttat tgtattcata tcaggagaga attattcctg ctgcaacaat aggcagcgaa
    28801 gggcaggtac tcgagtatgc caaaaaggtg acgattgtgg aaattaccga tgacagggtt
    28861 gaaaagatta aagatgctaa atatattagc tacgaaggac actctaattc aacagagtat
    28921 tacgacgaca gactggtaaa aatatacgga gagtaccgta ttaaattcga tttgtcgttt
    28981 gaagcagata tcgaaatgaa tgttgatata gatactattg aattttaagc catgaaaaaa
    29041 ctagtaatta tctcagcagt agtttttatg gcaatcaata gcgttgccca gcaggacttt
    29101 agcttatact acatgaacag aaccctgcaa agccaatggc tgaatccctc gtccgattat
    29161 ccgtatgctg taagtgtggg cggattattg gtgcctgtct ttggtcagat atctcctcca
    29221 ctgcatttta attacgccaa taatgcttgg cattataacg atattttaca catgggtacc
    29281 ggtataaaag ccgactccct ggttctcgac attgataaaa tgatgaataa tgtgcataag
    29341 caaaacaata tccgtttcga aaattatatt gaattattaa gcgtgggctt taaggtaaaa
    29401 accttgtttt ttacattttc tatctctgaa aaactgaact atggcgtatc gattccggga
    29461 gattttatgc acctgattgt atatgggaat atgccttatt atagcgacaa tagaagttat
    29521 gattttagcc ggctgaactt aaattttacc cactacagcg aatatgcatt gggcgtttcg
    29581 gccagtgccg acaagcttcg cataggagga aggatgaaat tactttttgg taaatcgaat
    29641 atcagcaccg atattaaaac cctgtcgttg tacaccaatc ctgaaaatgt ttacatgact
    29701 gcagttaccg atatgagtat acgtgcatcg caaccctttc caatcgaata cgaatactat
    29761 cccgatggcg acagtataag atttgattta cccgatgatt tgggaagtta ttttgaccct
    29821 gcagattatt tactgaaccc taagaatttt ggtttggctg tggatattgg agccagttat
    29881 gaaattaacc accaatggca ggtatttgcc agcgcaaccg acattggact gataacctgg
    29941 aacgacaatc ctttcagtct ggtcagcaac ggaagcttcc ttttcaaagg cattgaagcc
    30001 gattactggc atagtaccga tgaaatagaa gaatctcttg aaaaccttgg agactctatt
    30061 ctgagaacct ttcgatttga agagagcaat cagggttatg taactttttt gccctcaaat
    30121 ctgtatgttg gaggtatgta taaaccattt gataaaattc atataggagc actgtatcgt
    30181 gctgaattct ataaaaagag tattatgtca tctcttacac tttcgtttaa ttcgaatctt
    30241 acaaaatggt ttagtgggca tttgagttat tctattgcaa ataacagctt tatcaatttc
    30301 gggcttgggt ttacattgcg ccttgcaatt ttccagttct accttaccac cgacaatctt
    30361 acgggaataa tttggccgca aaagtcgcaa aacatgaatt tacggatggg ttgcaacatg
    30421 gtttttggtt ataaggaaaa acccgtatca aaaatatttt attaacctgc tttatgttgt
    30481 gatttcaaag gaatggaatt aaacaggctt acccgaagcg atattcatcg gtatgtttac
    30541 cttttggggt tgcttataat agcagcgttt ctgccatttt ctgtttatat tacaagtgtt
    30601 gctcattttg tgattattgc gaattactta ctcgaaggta attattatca gaaaatcagg
    30661 attctctttg cccgaccatc tgtagtccta tttttgctgc ttatagtggt tcattttatc
    30721 tggctgatta attcaactga catacattat gcgttaaaag acatcagaat aaagctacct
    30781 ttgcttgctt taccgataat ttttggcact accgcaccca taaaattcag acaactaaaa
    30841 ttaatattgc ttgtttttgc aggctcaatt ctgctgaact ctgtaatttc tacactatct
    30901 ctggctggag tttttgcaac ggaacaactt aacgatacgc gtgatatctc tttatttgtt
    30961 agccacatcc gcttttctct catgattgta ctaagcatat ttattttaat ttggtttatg
    31021 ttccgtcgaa aaattgttta tcggttcgaa tacgtcttat atagtactgt agctttgtgg
    31081 ctcatatcct ttttaataat aagccagttt ttcacaggaa ttgtactgtt ttttgtggtg
    31141 tgtattcttt ttctgctctg ggtttttttg caacaaaaac gacgaatttt aatgatatca
    31201 gggattataa ccctctttct gctacttgtt tcgtttgcaa cacttgggta ctttcaggta
    31261 aggagctttt attccccacc tgtcaggtcg ggaacaagct tgctgaaaac taccgaaaac
    31321 ggaaacccct attatcataa tatgtcgagt cggcttatcg aaaacgggaa tctcatttat
    31381 gttgaaatat gcgaaaacga aataagaggt gcctgggagc tgcgcagtac attatctatt
    31441 gattcccttg atgcaaaaca gcaaccggta attcacaccc ttatccgtta tctaagttca
    31501 aaaggaatgc ctaaaagtgg gaatgccgtg ctatcgttgt ctgatgaaga gattaaagca
    31561 attgaagcag gaaaatcaaa tgttaggtac ctagacggta tcgatttttt tgacaggata
    31621 tatcaattca tatggcaaat cgattgctat attaaggaag ggaacccaag cggacactct
    31681 ttaacccaac ggattgaata tgtgataacc ggtttgtata ttcttaagaa taacttttgg
    31741 tttggtgttg gtaccggtga tgttgattcg gttttcaaac aagcctacat cgaaaaaaac
    31801 tcaatgctcg aagaacagtt caggcatcgt gctcacaatc aggttcttac gttttatatt
    31861 gctttcggat gcatcggtgc aactctatgc ttaatagcca tattttttcc tttgatactc
    31921 gaatcgaaaa agagtcgctt tctgttgttg ctatttgttt tgattgctct ggtttcgatg
    31981 ttgaatgaag atacgcttga aacagcaaca ggttctgcct tttttgcgta tttttattca
    32041 ttgtttttat ggggagtaaa cgaatgaaat taaaaatctt tcattatgat cgataataac
    32101 cagcattaca tgcaatttgc aatagagatg gcattgaaaa acctgaaaag cggaggggga
    32161 ccttttggag ctgtcattgt taaagacgat gtaataattg caagttccgg caacagcgtt
    32221 actgttgata acgatccaac tgcacatgcc gaaatcaacg caatccggaa agctgcgaaa
    32281 gtgcttaatt ctttcgattt aagcggctgc accatttatt ccagctgtga accatgtcca
    32341 atgtgtctgg gtgctattta ctgggcccgc atcgatacat tgtttttttc ggccacgcgt
    32401 gtcgatgccg aaaatgcagg gtttagcgat gccatgattt ataacgaaat aggcatgcct
    32461 attcacaaaa gaacattgca aaccgagcac ctggtaaccg aaaatgctga attacctttt
    32521 gaagaatgga aaaatctgct caacaagacc ctgtattgaa aagactccca ttgtgttttt
    32581 atacccgcga tgcaaaaaca gttgctatcg agttgttggg taaaacaatt gttagaagac
    32641 attcggatgg agaaattgta aaatatatta taaccgaaac tgaagcctat ctgggtgagt
    32701 tcgatttggc ctgccatgcc agtaaaggga aaaccccaag aacggagatt atgtttacac
    32761 aaggcggagt actgtatatt tatcttattt atgggatgta ttggatgatg aacattgtta
    32821 ctggtttcga aaatcaggca caggctgtgc tgatcagagg aattgacaaa gcttcgggac
    32881 cgggaaaaac ggcggctctc ttacagctat caaaagaatt caacggagaa tcgttgcttg
    32941 aatcaaaccg catctggctt gaagactcga acttggtatg tcggtataag gcaacaccaa
    33001 gaattggagt ggattatgcc ggtgaattct ggaaatcgca attactcagg ttttgcgcaa
    33061 tagtttgagt ctactctaca aacgagtatt tgacacgaat tgaatgactt tggttgtaga
    33121 gaacacagtt ttgtttctca acgtgccgaa taaacccgat ttttctattc atgtttttta
    33181 ataactttgt gttctgatta attaagtata atggatatga ttgaccgaga atttgaaaga
    33241 gggtgtcaga ttgatatttt agatgataat acgggcatta taaataaatg ttgctataaa
    33301 ctaaacgtta acaactggtt aaatgacatt cccgtggcaa catcaggttt tgatgttgtt
    33361 gaggttcgtt ttaaaaatac aagaaaaggg tttttcagga acgtaaatca attaaacctc
    33421 cgcgaaggag aacttgttgc tgtagaatcg acccaaggac atgatattgg cattgtatcg
    33481 ctcaccggag aacttgtgcg ggaacaaatg caacgttatc atgtgaatta tgattccgat
    33541 tttaaaaaaa tataccgaaa agccaaacaa actgatttgt tgaaatggaa agaggctgtg
    33601 gctcaagaaa taccaacaat gctacgaaca agagagttgg caaaacaact gcaattgaac
    33661 atgaaaatcg gagatgttga gtatcagggc gataaaacga aagctatatt ctattacata
    33721 gccgatgaaa gggttgattt caggcagctg ataaagcata tggctgaaga gtttaaaata
    33781 cgtgttgaga tgaagcaaat cggcgcccga caagaggcag gaagaattgg agggattggt
    33841 ccatgtggaa gagaactctg ttgtaccaca tggatgacga attttatttc tgtgtcaaca
    33901 aactcagccc ggctacagga gatttctctt aacccgcaaa agcttgcagg ccagtgtagt
    33961 aagcttaagt gctgtctgaa ttttgaactt gaggtatata ttgatgcacg taaggatttt
    34021 atttatgata acaaaacgct tcacactaca caaggagatg ccaaacatat taaaaatgat
    34081 gtttttaaaa aaacaatgta cttcgagatc ttcaacaata atatacctgt ggtaatagcc
    34141 ttgcctctcc aacgagttaa agagattatt gaattaaata aaaagggtat tgctgttgat
    34201 aaacttattt ctgaagagaa caaagcgccc tccgtaaccg agcatgaatt taccaatgat
    34261 gcaaacaacg ggagcattac ccggttcgac tcgaaaaaca ggcaatcaaa taaaaaatca
    34321 cacaaaaaga atatgaaacc taataatcct gccactccgc taatgcttgc gatcgttttt
    34381 ttgataagct ctttttctat tagctgcgat agaaataaaa tttatgagaa atacattaaa
    34441 atcgataata tggtttggaa cgacaaaaac tatattcggt tcgaaaagga aattacagat
    34501 acggtatcac gacataatat ctacatcctt gttcgaaatg ccactcagta ccggtacgga
    34561 aatttgtggg tttttgtttc cacaactgct cccaatggga ataaggatac cgacacggtt
    34621 gagtgtgtgc ttgccgataa aaacgggaaa tggcttggcg atgggatggg tgatttatgg
    34681 gacaacgaga ttctttggaa acgaaatgta acattcccgt atcagggcaa ttacgttttt
    34741 caatttgaac agggaatgcg tattgaaaac ttgccgggaa tactggatgt ggggcttaga
    34801 attgagcgag tccctgagga gaaataaagc cgttactgca acatgggcca gaaaaaaaag
    34861 ttaaaacgat tcgccgaaaa caatttgttt agtaatgttt ttcagcctct ttacaccgat
    34921 ctcattaacg acgatttcgg gttaaaaggg aattggaagc agcagttttt taaaaacaat
    34981 aacccgatta ccgctgagct gggctgtggt aagggcgact atgttactgc actttccgga
    35041 ttgttacctt tgaataatta tattggaatc gatataaaag gagcccggat ttggaaagga
    35101 gcaaagtatg ctgaagacaa agcggtgcaa aatgtagcct ttttaagaac acgaatagag
    35161 tgttcaccgc gctgcttcga aaaaaatgag attaacgaaa tatggattac ttttcctgac
    35221 cctcagctaa accgcaaaaa gaggattaaa aagaggctta cgtctgcaag atttctgggt
    35281 tattaccaaa aaatccttat taacaacgga atcatacatg ttaaaaccga tgacgatgat
    35341 ttgtttgcat acactaaaaa agtaattgaa ttcaataatc tgacgcttga aaaagaaatc
    35401 cgtgatattt acaaggaatg cccggaggat aagattctta gcattcggac ctactacgaa
    35461 accaaatggc ttaccgaggg aaggaccatt aaatatcttt gtttccgctt acctgataca
    35521 cgcgatatag ttgaacctga cacattcgat gaagactgac tctcacaacg atttttttga
    35581 aaaagtatgg gctgttgcca cacttattcc ctatggaagg gttacatcgt atggcgctat
    35641 agcccgttac cttggacttc cgggttctgc acgaattgtc ggctgggcta tgaatcaatc
    35701 ccacatgaat ccggaggttc ctgctcatag ggttgtgaat agacagggtt ttttaacagg
    35761 aaaacatcat ttcttttccg aatcacttat ggcagaacta ttgacaaacg aaggcattgg
    35821 agtagaaaat gaccgaatcg ttgatttcaa aaagcatttt tggaacccct ctcaagaact
    35881 aatatagaac ttatttgtta taattttaga aacaagttac ggtatgattg taagtattga
    35941 aaaaatcagg gaagagttat cgaagttgca acacccgggt aaaagcacag accttgttag
    36001 tctgggtatg gtgcagcgaa ttgtggtaaa agaacaaaaa atcgaaatcg gactggtttt
    36061 cgataaagct acggatccat ttatgggttc cattgaaaaa aaatgtaaag aagtagttgc
    36121 agcaatggct ggcgataact ttacaattac ggttggtagt tctgtaaaga agcctgttgc
    36181 gcccaagaaa aaggagcacg ccgaaggact tgaagttaac aatataattg ctgttgcttc
    36241 aggaaagggt ggggttggca aatcgacaat tgctgccaat cttgctgtgg ctctcagtat
    36301 tgatggatat aaagttggct tacttgatgc cgatattttc ggacccagta ttcccaaact
    36361 ttttgggttg gaagcttatt cgccatcagt ggaaaaaatc gatggcagag atatgattat
    36421 tccggctgag aagttcggga taaaaatttt atcgataggt ttttttgtag atgccgataa
    36481 tgcattgatt tggagaggac ctatggcctc aggagctttg aaacaattaa ttaccgatac
    36541 ggcatgggga gaacttgatt ttcttataat agatacccct ccgggaacaa gcgatattca
    36601 tcttactctt gttcagacaa ttgcagtaac cggtgttgtt attgtaagta cacctcagca
    36661 agttgcactt gccgatgccc gaaaaggtat tagtatgttc cgctccgaag gtataaatgt
    36721 tcccgtgctt ggtctggtag agaatatgag ctggtttaca ccggcagaat tacctgaaaa
    36781 taagtattat atctttggta aagagggggt taaaaaactt gcagaggaac aaaacatacc
    36841 gctgttggca caaataccgc tgattcaatc gatatgtgaa ggtggcgatc agggccgccc
    36901 cgaccatgct ttgggacacg atgctcttgc cgaagcaatc agaaatatgg ccagcgaaac
    36961 tgtttcgcag ctacagaaac gtaatgcaaa tttgccccca acccaaatag ttgaaataaa
    37021 ctcaaaataa taaaattatg gaagaagtaa aaaagaggat tcagaaatcg attgatgaaa
    37081 tcagaccata cctgagggct gacggaggcg atattagctt tgtggacttt accgatgacg
    37141 gtgtagttaa agttaagctg ctcggcgcct gtggatgttg tccgcatagt attcagaccc
    37201 ttaagctggg tgttgaagcc aaattgatgg aagatgtacc cgaagtaaaa gaagttgtgg
    37261 cagtatagga tactgttcaa attcttaatg ccgaacgaat cgatggctat tctatttttt
    37321 cctgacttcg acacttttta ataggcaatt ttgtaagtgt cagatactga
//
LOCUS       DAOOQP010000013        36433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1342_length_36433_cov_5.862582, whole genome shotgun sequence.
ACCESSION   DAOOQP010000013 DAOOQP010000000
VERSION     DAOOQP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1342_length_36433_cov_5.862582"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLK75_02590"
     CDS             complement(<1..283)
                     /locus_tag="PLK75_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38166.1"
                     /translation="MKRIPLVLFVFVLMLQAALLYSQNRELSQQEQQQLMSYPELKMP
                     ESYKNKSIPYMVDNSQLEYFRPMFSQAGMSCGQASSTGILFTYEMNRARD"
     gene            complement(340..831)
                     /locus_tag="PLK75_02595"
     CDS             complement(340..831)
                     /locus_tag="PLK75_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOY38167.1"
                     /translation="MKLLTLVRHAKSDWDNDFQDFDRPLNHRGMHDAPAMGQWMKQML
                     PPADLFLSSSALRAKTTALLIASACGFPEDNIVFTDSLYHCGVRDYLKILEIQPENIQ
                     HIVVFSHNPGISTFSTYLCPRYPISFPTCGVVHLNIYANSWSEITKNSGKIEQYCFPS
                     MIK"
     gene            1073..2095
                     /locus_tag="PLK75_02600"
     CDS             1073..2095
                     /locus_tag="PLK75_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38168.1"
                     /translation="MKKAILILISFLISYSCFSQVLVPEASTWSHKSHQQNGPVLLDN
                     CHWQANFEEEIPLWSFEHSMGTHDWQVGDTTPDNGFEYDIDGDGVTETAPPLWVYMGR
                     RYVSDYSGSGDRFAYIDGVSDLVTGNPEIFEASICFNSINLSEAIHPKLSFYQNYKAF
                     NGDKTFVDFSTDNGETWTSMEVNTEVNVGAYGDNYYQMTAPVFIAGQDNVSLRFRMLT
                     TDATLVGSGYGWQIDDVAFSELPEYDLRLVDFFIHYNDAVFNYPEYTYISSYYGAVLC
                     TSFLSAISFSWIVKNNGYSEVTPSCFVYVYNKNDELLFYDNVQGGVLMSGCVDTIDYL
                     NSFDIS"
     gene            2186..3373
                     /locus_tag="PLK75_02605"
     CDS             2186..3373
                     /locus_tag="PLK75_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38169.1"
                     /translation="MMVISNYFARDNNCINGHIGPSSFDIGHKNGEMLGNAYIISSTV
                     WNPVSLQVFIDSLTSDSTSIRGHVFKFNINSESWDDIVQTTFYPINSTNIGEWVTLYS
                     SEDFGQIEVGYQDMMLLVFGIEFNYGDKEDNNIIYLGTDNNVHSSKWGSYWMFKDDVA
                     QNELITLSNWNYSGVAIRFVDSFFVGQIHYEAYICEGETYSFFNNNYSQTGVYYHEGE
                     DVLYLHVIDAPAVVNLIGCFDEESQSLEISMDGSELSVLYKLYCNLCGTDTLLNVFYG
                     TGLPMSLGTHYLNGDYHVLAKAEVPDCDRWMNGTVNFQVSAPALQSELDFSLYPNPAH
                     DVLLIETSHTEFEVYITDINRKEMFHGQNTKSVNTEMLAPGSYLVHLKTREHDSVQKF
                     IVK"
     gene            complement(3449..5485)
                     /gene="metG"
                     /locus_tag="PLK75_02610"
     CDS             complement(3449..5485)
                     /gene="metG"
                     /locus_tag="PLK75_02610"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOY38170.1"
                     /translation="MMKEFKRYLITSALPYANGPVHIGHLAGVYVPADIYVRYLRARN
                     HDVVFIGGSDEHGVPITLRARKEGLTPQKIVDRYHEIIKKSFADFGISFDIYSRTSNA
                     MHHETAAEFFTTLYEKGEFVEQANRQYFDEQEQQFLADRYIMGTCPKCGAENAYGDQC
                     EKCGSALSPDELINPRSTISGQAPVLKETKHWYLPLDKYEGWLRQWILEEHKEWKPNV
                     YGQCKSWIDAGLKPRAVTRDLDWGVPVPLDEAKGKVLYVWFDAPIGYISATRELTPDW
                     EKYWKDEDTRMIHFIGKDNIVFHCIIFPAMLKHEGSYILPDNVPANEFLNLEGDKIST
                     SRNWAVWLHEYLNDFPGQQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAILGNF
                     VNRALVLTQKYFDGKVPEADGNNEYDFQLRQLCSDYKTNIEENIGQFKFREALKELMN
                     IARLGNKYLADTEPWKLIKKDEDAVKTILYNSLQLCARLAVLMEPFLPFSAQKLKDML
                     CLPQLSWDDCKNFDLLLSGHSLGEPGLLFAKIEDAAIEAQINKLMQTKAANEAAALKP
                     APSKESIEYDDFIKLDIRIATIKEAEKVPKADKLLKIVLETGLDTRVVVSGIAQYYKP
                     EDIIGRQVCLLANLAPRKLRGIESQGMILMASEPDGTLKFVSPESIMVNGSTVS"
     gene            5812..6720
                     /locus_tag="PLK75_02615"
     CDS             5812..6720
                     /locus_tag="PLK75_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOY38171.1"
                     /translation="MIIPAPLQKGDTIHIVSPAGKIDPKQFYPAVENLERNGFHIRVG
                     EHVLSQHFQYSGTDAQRLMDVQAAFDDSEAACIICSRGGYGTIRLLPSIRLDGFMKKP
                     KWLLGFSDITILHAHVGNAGVASVHGAMTKSLSVAGTLSTDTALNALKGLGMYYLLPH
                     HEMNRAGKTEAVVTGGNLSVLYSLLGTPYFPETKGKILFIEDLNEYLYHIDRMMISLK
                     LAGVLQHISGLIVGSFSDMLDNKNPFGKNAYQIVAEHVADYSYPVCFGFPAGHTDDNR
                     SIVFGADTVFEVSNTKTELYTKDLNM"
     gene            complement(6770..8158)
                     /gene="glmM"
                     /locus_tag="PLK75_02620"
     CDS             complement(6770..8158)
                     /gene="glmM"
                     /locus_tag="PLK75_02620"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOY38172.1"
                     /translation="MTLIKSISGIRGTIGGVQGEGLTPVDICKFTAAYAQWMQHSNSA
                     KVGLTIVVGRDARISGPVVHNFVCNTLISMGISVIDLGLATTPTTEMAVILEKADGGI
                     IITASHNPRQWNALKMLNSKGEFLTAEEGILILELANKSDFSFAAIDSLGKITEILNY
                     ENKHIDEILKLTLVQPEVIRKARFKVVIDCVNSVGGIVIPKLLKALGVIDVVELYCNP
                     NGEFPHNPEPLPEHLNAISQKMTETGADVGFVVDPDVDRLAIVCEDGSMFGEEYTLVS
                     VADYVLQYTPGTTVSNMSSTQALRDVSAAYGCSCFSSAVGEVNVVAMMKQHQAVIGGE
                     GNGGVIYPELHYGRDALVGIALFLTNLAKRGTKVSQLRNGYPAYVISKNKIELNLGVD
                     VDKVLENVKKHYSNHNISTIDGVKVDFGSEWVHLRKSNTEPIIRIYAESQTREQADNL
                     ALSVIEKIKSYM"
     gene            8329..8523
                     /gene="rpsU"
                     /locus_tag="PLK75_02625"
     CDS             8329..8523
                     /gene="rpsU"
                     /locus_tag="PLK75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOY38173.1"
                     /translation="MIVIPVKEGENIDKALKRFKRKFEKTGVVKELRVRQAFTKPSIE
                     KRQQKLKAIYIQHLQEEVQK"
     gene            8613..9506
                     /locus_tag="PLK75_02630"
     CDS             8613..9506
                     /locus_tag="PLK75_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOY38174.1"
                     /translation="MMHIGLFLKYLKFEKRYSENTISAYNNDLLQFNSFIESETAGIS
                     VENASRNIIRSWIVSLASEKKDSPRSIQRKVTSLRSFYKYLLREGVIQKNPTVGIHPP
                     KTEKKLPVFLTQSQTDFFIDEDCFGDDFSGARDRAIIEMLYNTGMRLSEAAQMKHIDI
                     DLKSNQLKVLGKRNKERIIPFNKYLGEIIIKYINRKNEMGIGISAEDRFFVTDKGKPV
                     YVKFIYHKVRHYIGMVSTNKKKSPHVLRHTFATHMLNNGADLNAIKELLGHANLAATQ
                     VYTHSSFEKLKEIYKQAHPRA"
     gene            9528..9836
                     /gene="raiA"
                     /locus_tag="PLK75_02635"
     CDS             9528..9836
                     /gene="raiA"
                     /locus_tag="PLK75_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOY38175.1"
                     /translation="MKVTIHSLHFDADKKLLDFIDGKITKLSQISDEIISSDVVLRIE
                     NSETNENKVAEIKLKIPGANEIFAKKQSRTFEESIDSATNALRRQLRKTKEKQRTQKK
                     "
     gene            9945..10017
                     /locus_tag="PLK75_02640"
     tRNA            9945..10017
                     /locus_tag="PLK75_02640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9978..9980,aa:Thr,seq:tgt)
     gene            10035..10117
                     /locus_tag="PLK75_02645"
     tRNA            10035..10117
                     /locus_tag="PLK75_02645"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10069..10071,aa:Tyr,seq:gta)
     gene            10128..10200
                     /locus_tag="PLK75_02650"
     tRNA            10128..10200
                     /locus_tag="PLK75_02650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10161..10163,aa:Gly,seq:tcc)
     gene            10210..10281
                     /locus_tag="PLK75_02655"
     tRNA            10210..10281
                     /locus_tag="PLK75_02655"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10242..10244,aa:Thr,seq:ggt)
     gene            10332..11519
                     /gene="tuf"
                     /locus_tag="PLK75_02660"
     CDS             10332..11519
                     /gene="tuf"
                     /locus_tag="PLK75_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOY38176.1"
                     /translation="MAKEKFSRTKPHVNIGTIGHVDHGKTTLTAAITKALATDGLAVY
                     RDFDSIDNAPEEKERGITINTAHVEYETKNRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVNVPKIVVFLNKVDMVDDEELIELVEMEVRELLDF
                     YEFEGGTGPVIRGSALGALNSEPKWVEKVYELMAAVDEWIPIPPRDIEKPFLMPVEDV
                     FSITGRGTVATGRIETGVVNTGDEVHIIGLGAENRKTVVTGVEMFRKILDRGEAGDNV
                     GLLLRGIDKNEIKRGMVVAKPGSIKPHTKVKAEVYVLKKEEGGRHTPFHNNYRPQFYI
                     RTLDITGEIQLPEGVEMVMPGDNVTITINLIYEVACNVGLRFAIREGGRTVGAGQIIE
                     IIE"
     gene            11567..11639
                     /locus_tag="PLK75_02665"
     tRNA            11567..11639
                     /locus_tag="PLK75_02665"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11600..11602,aa:Trp,seq:cca)
     gene            11655..11849
                     /gene="secE"
                     /locus_tag="PLK75_02670"
     CDS             11655..11849
                     /gene="secE"
                     /locus_tag="PLK75_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOY38177.1"
                     /translation="MAKISAYFKETYDELAHKVTWPTWSELQTSAIVVMVASVIIAIV
                     VLAMDLIFRNGLEFLYGLFY"
     gene            11873..12418
                     /gene="nusG"
                     /locus_tag="PLK75_02675"
     CDS             11873..12418
                     /gene="nusG"
                     /locus_tag="PLK75_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOY38178.1"
                     /translation="MGDLEKKWYVVKALSGKEKKVKEYLENEVAKTKLTNQITQVLIP
                     TEKVYQVRKGKKISKERNFFPGYILIEAILDGEVEHIIRAVPGVLGFLGANDGKPIPL
                     RPNEVARILGKVDELTESSEEINVPFYTGEAVKVIDGPFNGFNGIIEEVHEEKKKLKV
                     MVKIFGRKTPLELSFMQVEKE"
     gene            12442..12885
                     /gene="rplK"
                     /locus_tag="PLK75_02680"
     CDS             12442..12885
                     /gene="rplK"
                     /locus_tag="PLK75_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOY38179.1"
                     /translation="MAKQIENFIKLQIKGGAANPSPPVGPALGSKGVNIMEFCKQFNA
                     RTQDRAGKVLPVIITVYSDKSFDFIVKTPPVAVQLIEIAKLKKGSAEPNRNKVGSVSW
                     EDVKAIATDKMPDLNAFTIESAMKMVAGTARSMGITVSGESPFKN"
     gene            12906..13601
                     /gene="rplA"
                     /locus_tag="PLK75_02685"
     CDS             12906..13601
                     /gene="rplA"
                     /locus_tag="PLK75_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOY38180.1"
                     /translation="MARISKNKKEALSKLDKTRKYDIKEAAKLVKEITYTKFDASVDI
                     DVKLGVDPRKANQMVRGITTLPHGTGKQIRVLVLCNPDKEQEAKEAGADYVGLDDYIE
                     KITNGWTDVDIIITQPMIMGKIGKLGRVLGPRGLMPNPKSGTVTQEIGKAVLDVKKGK
                     IDFKVDKYGIIHAGIGKVSFETEKIVDNASELVQTIVKLKPSTAKGTYIQSIFLSSTM
                     SPGIEIDPKSIVQ"
     gene            13621..14139
                     /gene="rplJ"
                     /locus_tag="PLK75_02690"
     CDS             13621..14139
                     /gene="rplJ"
                     /locus_tag="PLK75_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOY38181.1"
                     /translation="MKREEKDVLIEQLKEQIGMYSHFYLTDAAELNAQEVSDLRRKCF
                     ESDIKMVVVKNTLFKLALERAGKHYEELYPALQNNTAVLFCNTASTPGKLIKEFRKNH
                     EKPVFKAAYAEEMVFIGEDQLDALATIKTKDELIGDLIFLLQSPMKQLLSQLNSGKHI
                     VAGVVKTLAERE"
     gene            14175..14555
                     /gene="rplL"
                     /locus_tag="PLK75_02695"
     CDS             14175..14555
                     /gene="rplL"
                     /locus_tag="PLK75_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOY38182.1"
                     /translation="MADLKKLAEELVNLSVKDVNELASILKNEYGIEPAAAAAVVAAP
                     TAGGAAAAVVEEQTEFDVILKAAGAAKLQVVKLVKELTGLGLKEAKDLVDAAPKPVKE
                     KVSKDEASSLKAKLEEAGAEVEVK"
     gene            14652..18458
                     /gene="rpoB"
                     /locus_tag="PLK75_02700"
     CDS             14652..18458
                     /gene="rpoB"
                     /locus_tag="PLK75_02700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOY38183.1"
                     /translation="MAIKRNERINFSSNQNQLEYPDFLEIQLKSFREFFQIESTAEQR
                     KNELLFQVFQENFPISDTRNNFVLEFLDYSVDLPRYSIQECVERGLTYCVPLKAKLKL
                     YCTDPDHEDFETTIQDVWLGTVPYMTPFGTFVINGAERVVVSQLHRSPGVFFGQSIHT
                     NGTKLYSARIIPFKGSWIEFATDINSVMYAYIDRKKKLPVTTLLRAIGFESDKDILEI
                     FDLAEEIKVSKTALKKVVGRRLAARVLRTWTEDFVDEDTGEVVSIERNEVIVDREVVL
                     TNEHVDLIVDAGAKMVLLHKENQKTSDYAIIYNTLQKDPCNSENEAVVYIYHQLRNSE
                     PPDEATARDVIDKLFFSDKRYDLGEVGRYRLNKKLNLEIPMETKVLTRDDIIAIIKYL
                     IKLINSKADIDDIDHLSNRRVRTVGEQLASQFGVGLARMARTIRERMNVRDNEVFHPI
                     DLINSKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRMSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLISSLCVFAKINKLGFIETPYRQVENGKVQIDGDVL
                     FLSAEEEERKIIAQANAKLDPDGTFSNTRVKARLEADYPLAEKESVHLMDVAPNQIAS
                     IAASLIPFLEHDDANRALMGSNMMRQAVPLLNPEAPIVGTGIEYEVARDSRILVIAEG
                     NGVVEYVDAKEIIVRYDLTDEERFVSFHDDVTAYNLQKFQKTNQNTCVNQKPLVKKGE
                     RVVKGQILSEGYATRNGELALGRNLKVAFMPWKGYNFEDAIVISEKVVRDDYFTSIHI
                     EEHQLEVRDTKRGLEELTSDIPNVSEIATKDLDENGIIRIGAYVKPGDILIGKITPKG
                     ESDPSPEEKLLRAIFGEKAGDVKDASLKASPSTNGVVINKKLFSKTIKDRKTKNQDKV
                     LLDAIESEFNKKADALKEALVDKLFFIVNGKTSQGVKDNLNNDVIPKGNKFTQKLLFE
                     VDYINVNPCKWTTDKKKNDIIEKLLFNYSIEYKKIEGEARRKRLNISIGDELPAGIVQ
                     LAKVYLASKRKITVGDKLAGRHGNKGIVAKIVRQEDMPFLEDGTPVDIVLNPLGVPSR
                     MNLGQIYETVLGWAGVNLGLKFATPIFDGATIEQINDYTEKAGIPRFGKCYLYDGGTG
                     MKFDQPATVGVIYMLKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVW
                     ALEAFGASNILREILTIKSDDVIGRAKTYEAIVKGDPMPDPGVPESLNVLLHEMRGLG
                     LNVELE"
     gene            18487..22755
                     /gene="rpoC"
                     /locus_tag="PLK75_02705"
     CDS             18487..22755
                     /gene="rpoC"
                     /locus_tag="PLK75_02705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOY38184.1"
                     /translation="MAFRKDLKVKSNFTKISISLASPEDILDRSSGEVTKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LEVPVAHIWYFKTLPNKIGYLLGIPSKKMDSIVYYERYVVIQPGIKNADGVNQLDFLS
                     EEEYLAILDTLPKENQMLPDDDPNKFIAKMGAEAIFDLLTKINLDELSYDLRDKAGKE
                     TSLQRKSEALKRLSVVEAFRESEGRNKPEWMIVKVVPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTEANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVERRDSVIWDILENVLKGHPVMLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLVCTAFNADFDGDQMAVHLPLGNEAIIEAQLLMLASHNILNPANG
                     APITVPSQDMVLGLYYITKKRRGALGEGLTFYGPKEVIIAYNEAKLGLHAIIKVKVDD
                     IDKDGNYINHLIETTTGRVIFNQSVPREVGYLNEVLTKKSLRSIIGTVLKKAGTAATA
                     KFLDDIKDLGFSMAFKGGLSFNLDNVIVPESKQKMIEEGYRAVEEIRSNYESGFITNN
                     ERYNQVIDVWTHVNANLAKEVMKQLSEDNQGFNSVYMMLDSGARGSNEQIRQLCGMRG
                     LMGKPQKSISTGAEIIENPILSNFKEGLSILEYFISTHGARKGLADTALKTADAGYLT
                     RRLVDVAQDIVINEEDCGTLRGLVATTIKKNEEVVETLYERILGRTTVHDVYHPQTGE
                     LIVAAGQEITEDVADIIDSSPIEEVEIRSVLTCESKRGVCAKCYGRNLATGRMVQKGE
                     AVGVIAAQSIGEPGTQLTLRTFHVGGVAGNIATESTIFVKYEGIVEIEDLRTVEYVNE
                     QGEKYDIVIGRMGEMRLVDKNTGIIIMTNNIPYGSKLFVKPGQFVEKNTVVCSWDPFN
                     SVIITEVAGKVDFENVIEGITYREEADDQTGYREKVIIETRDKSKIPVVKIITGKNEV
                     LKTYNLPVGAHIIVQENEKVVAGKILVKIPRVVGKGGDITGGLPRVTELFEARNPSNP
                     AVVCEINGQVEFGKIKRGNREIIIRSKENEEKRYLVPLSKQILVQDSDFVRAGTPLSE
                     GAITPIDILNIKGPTMVQEYIVNEVQEVYRMQGVKINDKHFEVIVRQMMRKVEIEDPG
                     DTRFLEKQIVDKWEFREVNDAIFDKKVVIEPGDSSTAKAGQIISARKLRDENSILKRK
                     DLQPIMARDAVPATSSQVLQGITRAALQTKSFMSAASFQETTKVLNEAAISGKVDPLD
                     GLKENVIVGHLIPAGTGLREYSTYLVGNKEEMAQVSEKYEHVNE"
     gene            22761..23084
                     /locus_tag="PLK75_02710"
     CDS             22761..23084
                     /locus_tag="PLK75_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOY38185.1"
                     /translation="MEDKNLKQNQISIELKEDVAQGTYSNLAVITHSSSEFVIDFVRI
                     MPGVPKAQVKSRVILTPEHAKRLMMALKDNIQKYEQMNGPIKVNESSQGPVIPMTFGG
                     PTAQA"
     gene            complement(23135..23413)
                     /locus_tag="PLK75_02715"
     CDS             complement(23135..23413)
                     /locus_tag="PLK75_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOY38186.1"
                     /translation="MTKKDTQIQNSGNPSENLSLDNILVLHNDDFNTFDFVIECLVTI
                     CDHDDLQAEQCAFLTHYKGKCEIKKGKFDILKPMKDSLVEKGLNVTLE"
     gene            complement(23435..29701)
                     /locus_tag="PLK75_02720"
     CDS             complement(23435..29701)
                     /locus_tag="PLK75_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38187.1"
                     /translation="MTQVYKIVWLFSIFTLIIFRFSGFSQELSVKLLPGEQKVFEQAV
                     YEFESESYLSAKERFSQLLSLYPREIYFNYAYGACLVCLNTEFTKSIDYLEYALDGGM
                     YQANYFLGMAYHLRYMFDNAIRYYEDYQQRATGKDIKLLPVDRQIKMAQNGIDLIRYS
                     YDLQVVESKNVKTDNFYYSYNSNSRSGEIVVIPEAFKTKNDKKRNYSGLMYVSKLYNI
                     AFFSSYGTEKKSNLDLYMVVKEGDAWSAPKKLPKIVNSVEDESFPFFHPNGEFLYFSS
                     KGHNSMGGYDIFRVAYNRESDKWSEPENLDFPINTPLEDIMYITDDRDLTAFFASTRE
                     AEAGRIGVYQILIEKNPAQRQIANIDDVRKTSHLQINPIAVKEFEKRSQALIALNNDT
                     ASEFNNQIPDINTPSNDTATTDKLHEANTNIDSLQKRAEFYTIKNEATLSQIQQYMDE
                     IDRLEVEKQLIAGKNIPAEEKNKQISNIDAKIYVYTDSLAVLKDLSDNYELTINEMKQ
                     KSDYYNTQITEIENHNTENPTNEKSLDIINTEVKKELTVNPGSDFLENLQKEIEAKNN
                     QIRTNHDYLQSVERRINEIDSQIMDLNDSLLFTNDPEKQQAARKQIEMLKTERNRLVV
                     DYKERFLQQEFLIKETRQLEKEQAIAASLVNEPNVNYPKTGLSAEIVLNKAEKYIARR
                     DQRNLKDLAVMQNQVLDSVFFDNSTLANNRILAENNMLHEKTLHEKNNIQKIHIITES
                     PLFDESLVNTYLATADSLGAELEHTQKMFESETNIEKRNELLNNYNNTLAELNEVYTH
                     INDEITVVNNTIAVNNVSNENVDDIRDKTESQIAIQKEKGLSNDVILAEDYLTTADSL
                     YAMAAEADDKLPALQDYYLKEAIIYEAKAFEITHNNLTANNQNNFNNTNQNETINSDT
                     LLVYQPDSLSIDSIINETKQQIQIIENTYSNQNNDSIWDKTIAGYNELDEINSAIRNT
                     SDDNTKISLQDYATQIFKETRDLHAQAAEQLLNKNLEEYLIFDKSFRETSDHSISKAN
                     TPEALDRRNSAIALHNQAANLLIAAEETTNDSLKNELIIEAAQLESQAVQEYKKAFEI
                     AIDTNFYTSVKEPEYSFVTSNLTIDTIVSEYIEIRNELPQSNTNLQSTNEEEIVMQQI
                     DSLLIKAENINTSIASLQTEYEKTDIPSEQNRIINEIDSLRNEQEIIVDSAYSTLVDL
                     QQQKIRKNRDFIEANKANYTDRKKLYLEIEDDYLSYSKAISDKAYRSETNYFYLLQEI
                     VVFGDSILAKQQQLISEKTNNTQPENKTITSRIEEIGRKMLKTDNWVAENVQRQATSF
                     ENQRNNLTVPSEAQKNYMQNSERTLTSNMHIINNNPEIKSIVTRADSAMNEAATIRKE
                     LSDNFQTYSNNEIEARVARADSLETLGLAYQNKAIELIEVDKQLAIAREDSVRYAQKE
                     AAWNDTFNVNRNLLDTISTTSTEINSAIEKAIIVSDSLMNEATKIRDNISDNNNRFTE
                     NEKNQLQQHADSLTTAAIDLQNQAIKFSTNNDIQSAQLINSNYSYLIENPIRDSIVMT
                     YRPISKESLFLLPENEIDANKFLNNTTDREVIDKLTKEREELRLQIKSIDEEILLVQD
                     YISKTSNATDKTKLIAERDSLLTDRSQKVEKYLATNDDILNYSISKLEEKRLKLKTDS
                     PALKQQADSLTKDARRKEQQADSLRIAAMQSNDIINSKSDLLAQANALDEEALSQRSE
                     AIMILESPAADTVQQNTVLNGNNTNNTPASENVLVFRIQFAAYNRIVGDEYTKGLSPL
                     FTEKVPDRQLIRYMTGDYPNFETAARALPGVKNVGFNDAFIVAYLNGKRITIFEANQY
                     LAQQKVSKNEIIAVNDQQQATNQNVTDSVEKANSQYGIIDVKAVEGVFLCVQFAAAKR
                     ILLPGEIFGLSADFLEFLDNGYIRHLSGKFYSMNDAANARNIIRATGANDAFITAYRN
                     GVRIPVNEALQLIASQPSGSTDTATIAVHKTNTPNPAINTESFVIEIYVQIGAYRSEV
                     NTEIYNMFKNIAGTNNITTVQRNNLIIYRIGPLQSKATANEVRITAVEAGITDAFIVA
                     YKNGKQIPLREIND"
     gene            29909..30367
                     /gene="bcp"
                     /locus_tag="PLK75_02725"
     CDS             29909..30367
                     /gene="bcp"
                     /locus_tag="PLK75_02725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOY38188.1"
                     /translation="MQKLKLGDKAPDFKGITQSGKIVALSDYSGKKLILYFYPKDNTP
                     GCTAEACDLRDNYEMWQHKGYQVLGVSADSTKSHQGFAEKYNLPFDLISDTEKQILKA
                     YGAWGEKKLYGKSYEGVLRTTYIISENGIIEQIFEKVDTKNHSQQIIKNL"
     gene            30373..31386
                     /gene="recA"
                     /locus_tag="PLK75_02730"
     CDS             30373..31386
                     /gene="recA"
                     /locus_tag="PLK75_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOY38189.1"
                     /translation="MAKNTNEDIKLEKLKALQLTLGKIEKDYGKGAIMKLGDKVVENV
                     PIIPSGSVTLNYALGVGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKSGGIAAIIDA
                     EHAFDRTYAEKLGVDTQNLFISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPKA
                     EIEGEMGDSKMGLQARLMSQALRKLTANISKTQTCCIFINQLRDKIGVMFGSPETTTG
                     GNALKFYASIRLDIRRISQIKESEDVTGSRTRVKVVKNKMAPPFKKAEFDIVYGEGIS
                     KIGEIIDLSVDYEIIKKSGSWFSYGDTKLGQGRDAVKQLLLDNPELAEELEKKVVARL
                     EAN"
     gene            31405..32442
                     /locus_tag="PLK75_02735"
     CDS             31405..32442
                     /locus_tag="PLK75_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38190.1"
                     /translation="MTKLRNIFVVSGILISYVLIFLALNSCSCNSGKQKALTVANDSV
                     DSIKILSAQIRSNPRQHSLFYKRALLNYGNGNLQEAVNDIEIALKLDSINPVYLHTAA
                     DYFLLLGKSERSRDALLLCIKHHPQDKEAHFKLAQLYYYVKQYDDAIKEIAFIEGMNQ
                     QSDKTYFLKALIFKENNMTVKAVESLLSVLEYNPKHAEAYNMIGMIYYEADNKLAVDY
                     FNTAVLLFPDNLQILLNAGLVYEKFGLNDKTRQVFEKAISVDSTDYQANYNMGYYLLV
                     YGDDVKAAIGYFSKAVEIDPKAVNAYYNRGLAYELSGNETEARNDYNKALEIAPNYDL
                     AVEALNKLDKR"
     gene            32467..34569
                     /locus_tag="PLK75_02740"
     CDS             32467..34569
                     /locus_tag="PLK75_02740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY38191.1"
                     /translation="MRSIAFSVLLFSIFLVFESCIQNPVEKSEKTPVNVSLTDAEKKA
                     AVLTPEIMWKFGRIGEMQLSHDGKEVLYTVTFYNIEENRGYTHIFKMSTEKPAPVALT
                     SGPYNCYNVRWAPDAKKIFYISDKTETPQLLSMNADGSNKEVCDTLLNGLSSFEISPD
                     GTKILFCKEVAVGKSAKDLYTDLPKVNVVIAEDLMYRHWNHWNDNLWSHIFVAGFNNG
                     KIDNATDIMPDEAWDAPLSPYFDNTEMVWSSDSRKIAYTCKKLTGKEYALSTNSDIYE
                     YDLAGKTTLNVSNGMNGYDKYPVYSPDLKYLAWLSMETPGYESDKDRIIIKNLATGEI
                     KNMSANFDQPASSLLWDENGKFLYFISVINGTHQIYTLNIEDGSIRKVTEGIHDYTMI
                     AKAENVLLGAKMSMSMATELFRINPDGSETQLSFVNKEIYDNITFGKVQERWITTTDN
                     KKMLTWVIYPPDFDSTKTYPALLYCQGGPQSAVSQFFSFRWNFQIMAAGGYIVVAPNR
                     RGVPGFGQEWNRQISGDYGGQNMKDYLSAIDALAKEPYIDENRLGAVGASYGGFSVFW
                     LAGHHEGRFKAFIAHCGMFNLESQYGATEEMFFANFDLGGPYWDKNNAIAQKSYANSP
                     HRFVDKWDTPILIISGGKDFRIPYTESMQAFNAAQLRGIPSKLLIFPEETHFVLKPQN
                     AILWQREFRSWLDTYLKK"
     gene            complement(34640..36430)
                     /gene="rho"
                     /locus_tag="PLK75_02745"
     CDS             complement(34640..36430)
                     /gene="rho"
                     /locus_tag="PLK75_02745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOY38192.1"
                     /translation="MNTPSYRDFTLFPTSKPEEATKISSEDAKSENSGTPDNKDVIIP
                     HRKRRPVLPEEKTQDESALITKINQSIEPEKKEKKENPEAPENKKRKKRFRKKYENVS
                     VSSNPPAVNPVTAPSEIIANTHIVNDQVIHQPSIIADVVSEAEPIVDRKELTPPVKHV
                     ASVEPLQAKRQEIRLPEREVKKEISDSADKNEVNSLHENQKNTETSEIANHTEEPVKE
                     TQSFEFNGIVNIDGVLEIVPDGYGFLRSSDYNYLNSPDDVYVSQSQIKLFGLKTGDTV
                     EGSIRPPREGEKYFPLIKINKINGRDPSVMRDRIPFDHLTPLFPTEKFNITGQGKATL
                     STRIVDLFSPIGKGQRGLIVAQPKTGKTVLLQEIANAIAANHPEVYLMILLIDERPEE
                     VTDMARNVNAEVIASTFDEPAERHVRVSTIVLEKAKRLVECGHDVIILLDSITRLARA
                     YNTVAPASGRVLSGGVDSNALHKPKRFFGAARNIEGGGSLTILATALTETGSKMDDVI
                     FEEFKGTGNMELQLDRKLSNRRIYPAVDITASSTRREDLLIPAEVLNRVWILREFLAD
                     MNSLEAMDFMRQRLPNTKDNLEFLMTMDKL"
BASE COUNT        10757 a         6998 c         7927 g        10751 t
ORIGIN      
        1 catcccttgc cctgttcatt tcgtaagtaa acaaaatgcc agtgctcgaa gcctgcccac
       61 aactcatccc tgcctgtgaa aacataggtc tgaaatattc cagttgcgaa ttgtcgacca
      121 tgtagggtat cgatttattc ttgtatgatt caggcatttt cagttcaggg taactcatga
      181 gttgttgttg ttcctgctga ctgagttccc tgttttgcga atacagtaat gctgcttgca
      241 acatcagaac aaaaacaaat aaaaccaaag gtattctttt cattgttgag agtttatgcg
      301 catatagacg ttttggattg tttttagttt gctttacatt tattttatca tcgatggaaa
      361 acaatattgt tcaatttttc cgctattctt cgttatttcg ctccaggaat tggcatatat
      421 attcaaatgc actacaccgc aagtcgggaa acttatggga tatctcggac agagatatgt
      481 cgaaaaagtg gagatgcctg gattgtgtga aaagacaaca atatgctgta tgttttccgg
      541 ctgtatttca agaattttta aataatccct cacaccgcaa tgatacaaac tatcggtaaa
      601 aacaatgttg tcttccggaa atccacaggc cgaagcaatt aaaagagcgg ttgtttttgc
      661 ccgcagtgcg ctgctcgaca gaaacagatc agccggcgga agcatttgct tcatccattg
      721 ccccatggca ggcgcatcat gcataccccg atggttcagc ggtctgtcga agtcctggaa
      781 atcattgtcc cagtcgcttt tggcatgtcg cacaagggtg agtagcttca tctggcaggg
      841 tttgttcaat gagctaaaat tacaaaatat tatgatatga ttttagcaaa ctgcatataa
      901 tcagacaaat tgaaaaacat gtcaaataaa tactgactga aagtaataaa aacttacaac
      961 tgcaatataa cgtgtttata tactacagga tagatgccta ctttttttgt tatatttagt
     1021 gtaattttgt tttaattatc attaaatttg tagaaacact aatttagttg ctatgaaaaa
     1081 ggctatatta atattgattt ctttcttaat atcttatagc tgtttttctc aggtgctggt
     1141 tccagaagca tcgacttggt cacacaaatc acatcagcaa aacggacctg tattgctcga
     1201 caactgtcat tggcaagcaa acttcgagga agaaatacct ttatggtcgt ttgagcattc
     1261 gatgggtacg catgactggc aggttggcga tacaacccct gacaacggat ttgaatacga
     1321 tatagatgga gacggagtca ccgaaactgc tccaccgctt tgggtttaca tgggccggcg
     1381 ctatgttagc gactattcgg gttcaggaga ccgttttgcc tatatcgatg gtgtttcgga
     1441 ccttgtaaca ggtaatcccg agatatttga agcaagtata tgtttcaact ccattaatct
     1501 atcggaagca attcatccta aacttagctt ttatcagaac tacaaggcat tcaacggtga
     1561 taaaactttt gttgatttca gtactgacaa cggtgaaacc tggacttcaa tggaagtaaa
     1621 tacagaggtc aatgtaggtg cttatggtga taattattat caaatgactg cccctgtatt
     1681 tattgccggg caggacaatg tgagccttag attcaggatg ctcacgacag atgccacact
     1741 ggttggcagt ggttatggtt ggcaaatcga cgacgtagct tttagcgagt tgccagaata
     1801 tgatttgaga ttagtggatt tttttattca ttataatgac gcagttttca attatcccga
     1861 atatacatat atttcgtcgt actatggtgc tgttctttgt acttcttttt tatctgcaat
     1921 atccttttcc tggattgtaa aaaacaatgg atattccgaa gtgactcctt catgttttgt
     1981 atatgtttat aacaaaaacg atgagctatt attttacgat aatgtgcagg gaggtgtatt
     2041 gatgtcaggt tgtgttgata ctattgatta tctcaacagc ttcgatattt cataaataga
     2101 atgtttcgac acccttaaaa taattttagg ctcaaacccg gtagcagcca gcgattattc
     2161 tccgctaaac aatgctgata ctacaatgat ggtaatttct aattattttg cccgcgataa
     2221 taattgtata aatgggcata tcggtccatc cagcttcgat attggacata agaatggaga
     2281 gatgctgggc aatgcatata taatttcgtc aacagtttgg aacccggttt ctttgcaggt
     2341 ttttatagac tcactcacca gcgacagtac ttcaattagg gggcatgttt ttaagtttaa
     2401 cattaactct gaatcatggg acgatattgt gcagactaca ttttatccta ttaatagtac
     2461 aaatattggg gaatgggtga cactatattc ttcagaagat tttgggcaaa tcgaagttgg
     2521 atatcaagat atgatgctac tggtttttgg tattgaattt aattatggtg acaaagaaga
     2581 taacaatatc atttatttag gaaccgacaa caatgtacat agcagtaaat ggggtagtta
     2641 ttggatgttt aaagatgatg ttgctcagaa tgagttgatt actttatcaa actggaatta
     2701 ttccggggtt gcgatccgtt ttgtggatag cttttttgtt ggacagatac attacgaagc
     2761 ttatatctgt gaaggagaaa cttactcgtt tttcaataat aattattcac agacgggtgt
     2821 atattatcat gaaggggaag atgttttata tctacatgtt atcgatgccc ctgcggttgt
     2881 gaaccttatt ggatgctttg acgaagaaag ccagtctctc gaaatcagta tggacggcag
     2941 tgaacttagt gttctgtata agctctattg taacctttgc ggaaccgata cgcttttaaa
     3001 tgttttctat ggcactgggc tgcccatgag cttgggtacc cattatctca atggtgatta
     3061 tcatgtgttg gctaaagccg aagtacccga ctgcgatcgc tggatgaacg gcacagtcaa
     3121 ttttcaggtt tccgctccgg ccttgcagtc agaattagat ttttcgttat atcccaatcc
     3181 ggcacacgat gtacttctaa ttgaaacaag ccataccgaa ttcgaggttt acattacaga
     3241 tattaacaga aaagaaatgt ttcacgggca aaacaccaag tctgtcaaca ccgagatgct
     3301 tgcccctggt agctatctgg ttcatttaaa aacccgcgaa cacgattcag tacaaaagtt
     3361 tattgttaaa taaaggatat actgtactga atatcacgtt actaaattgc aaaaagcatt
     3421 atgtattttg cttaattgcc tgatagcttc aactcaccgt tgaaccattc accattatgc
     3481 tttccggact cacaaatttc agggttccat cgggttccga ggccatcaaa atcataccct
     3541 gcgactcgat gccgcggagt ttgcggggtg ccagattggc cagcaggcat acctgtctgc
     3601 caataatgtc ttcgggtttg tagtactggg ctattccgct caccacaacc cgggtgtcga
     3661 ggccggtttc gagaactatt ttgagaagtt tatcggcttt gggtactttt tcggcttcct
     3721 taatggtggc aatacgaata tcgagtttga tgaaatcgtc gtattcgatg ctttctttgg
     3781 atggagccgg tttcagtgcc gcggcttcgt tggcggcttt ggtctgcatc agtttgttta
     3841 tctgggcttc gatggcagca tcttcgattt tagcgaaaag caaaccgggt tcacccagac
     3901 tgtgacccga cagcaacagg tcgaaattct tacaatcgtc ccaactcagt tgcggcaggc
     3961 acagcatatc tttcagcttc tgagcactga aaggcaggaa aggctccatc agcaccgcca
     4021 gacgggcaca caattgcagc gagttataca agatggtttt caccgcatct tcgtcctttt
     4081 ttatcagctt ccatggctcg gtgtcggcca ggtatttatt cccgaggcgg gcaatattca
     4141 tcagttcttt cagggcttcg cggaacttaa actgcccgat attttcctcg atgtttgttt
     4201 tataatcgct gcaaagctgc cggagctgaa aatcatattc attgttgccg tcggcttcgg
     4261 gtactttgcc atcaaaatat ttctgcgtaa gcaccagggc tcggttcacg aagttgccta
     4321 aaatggctac cagttcgttg ttgttgcgtg cctgaaaatc tttccacgtg aagtcgttgt
     4381 ccttggtttc gggagcattg gcggtgagca cgtagcgcaa cacatcctgt tgccccggaa
     4441 aatcgttgag atactcgtgc agccataccg cccagttgcg cgaggtactg attttatcgc
     4501 cttcgagatt cagaaattcg ttggcaggca cattgtcggg cagaatgtag ctgccttcat
     4561 gcttcagcat ggccgggaat ataatgcaat ggaacacgat attgtctttc ccgatgaagt
     4621 gaatcatacg ggtgtcttcg tctttccagt atttttccca gtcgggggta agttcacggg
     4681 tggccgaaat gtatcctatg ggtgcatcaa accacacata cagcaccttg cctttggctt
     4741 cgtcgagggg aacgggaacg ccccagtcga ggtcgcgggt aacggcgcgg ggtttcagtc
     4801 ctgcatcgat ccacgacttg cactgaccat acacattggg tttccactcc ttgtgctctt
     4861 cgagtatcca ttggcgtagc cagccttcgt atttgtcgag aggcagatac cagtgtttgg
     4921 tttctttcag aaccggagcc tgaccgctga tggtggaacg cgggttgatg agttcgtcgg
     4981 ggctgagggc agatccgcat ttttcgcatt ggtcgccata ggcattttcg gcaccgcatt
     5041 tggggcaggt acccatgatg tagcggtcgg caagaaactg ttgctcctgc tcgtcgaaat
     5101 actggcggtt ggcctgttcc acaaattcgc ctttttcgta aagtgtggta aaaaattctg
     5161 ccgcagtttc gtggtgcatt gcattgctag tgcggctgta aatgtcgaac gaaatgccaa
     5221 aatcggcaaa ggattttttt atgatttcgt gataacgatc aacaatcttc tggggtgtaa
     5281 gcccttcttt acgggcacgc agggtaatgg gaaccccgtg ttcgtcgctt ccgccgatga
     5341 aaaccacatc gtggttgcgg gcacggagat agcgcacata aatgtcggca ggcacatata
     5401 caccggccag atgtccgata tgtaccggtc cgttggcata aggcagtgcc gaagttatca
     5461 ggtatcgttt gaattctttc atgatgcaat ttgtaaaact gcgcaaatat aatcaaacgc
     5521 tgcgagtttt cgaaaagagg ttttgccgcc tgtttttccg taatctagtc ctcgtttgca
     5581 acgaggactt atttattggt gcatttctaa tgcactacaa ttaacgcatt ataaatgctt
     5641 atcataaatg cgacctcgtt acaaacgagg tcgagatgtt tttttgtcaa aaaaagcttt
     5701 aattcgaatg aattgtaaaa aatcacaatc tgttatgaaa caggcagttt aattttgtaa
     5761 gtgattaaaa attaatgaat tttacactgt aaatatgttg aaatacaaat tatgataata
     5821 ccggcaccgc ttcagaaagg cgataccata catattgtga gtcctgcagg aaaaattgac
     5881 ccgaaacaat tctacccggc tgttgaaaac ctcgaaagga acggctttca cattcgggtg
     5941 ggtgagcatg ttctttcgca gcattttcag tactcgggga ccgatgctca acgcctgatg
     6001 gatgtgcagg ctgccttcga cgacagcgaa gctgcctgta ttatctgctc gcggggtgga
     6061 tatggtacaa tcagactgtt gccttcaatc aggttggacg gctttatgaa gaagcctaaa
     6121 tggctgctcg gtttcagcga tattaccata ctccatgcac atgttggaaa tgcaggggtg
     6181 gcatcggttc acggagccat gaccaaaagc ctttcggttg caggaaccct ttcaaccgat
     6241 actgccctga acgccctgaa aggattggga atgtactatc tattgccgca tcacgaaatg
     6301 aaccgcgccg gaaaaacaga ggcagtcgtg accggcggaa atctgtcggt gctgtattcg
     6361 cttcttggaa caccatattt ccctgaaacg aagggtaaga tactatttat agaagacctt
     6421 aacgaatatt tgtatcatat cgacagaatg atgatcagcc tgaaactggc gggtgtgctg
     6481 caacatattt cagggcttat cgtgggcagt ttcagcgaca tgcttgacaa taaaaaccct
     6541 tttggaaaaa atgcttacca gatcgttgcc gaacatgttg cagactattc ctacccggtt
     6601 tgttttggct ttccggcagg ccataccgac gacaaccgca gtattgtttt tggtgccgac
     6661 acggtttttg aggtcagcaa tacaaaaaca gaactatata ccaaagattt aaacatgtag
     6721 aaacatttgc ttttctgcca caataagcag gattctacaa aaattggtat tacatatagc
     6781 tttttatttt ttctattacc gacagtgcaa gattgtcggc ctgttctcgg gtctggcttt
     6841 cggcatatat tctgataatg ggctctgtgt tcgacttacg caggtgcacc cactcactcc
     6901 caaaatcaac tttaactccg tcgatagtgc tgatgttgtg gtttgagtaa tgttttttta
     6961 cattttcaag caccttatcc acatcaacac cgaggttgag ttcaatttta tttttagaga
     7021 tcacatatgc agggtacccg ttgcgcagtt gcgatacctt ggttcctctc ttggcaagat
     7081 ttgtcagaaa caatgcgata ccaaccagtg catcacgtcc gtaatgcagt tcaggatata
     7141 tcacaccgcc gttgccttca ccaccgatta ccgcctgatg ttgtttcatc atagcaacca
     7201 cgttcacttc gcctactgcc gacgaaaaac aagagcagcc gtaggcagcg gacacgtcgc
     7261 gcaatgcttg tgtcgacgac atattactta ctgttgtgcc gggggtgtat tgtaagacat
     7321 aatctgcaac cgaaacgagg gtgtattctt cgccgaacat gcttccatct tcgcaaacaa
     7381 tcgccagcct gtcgacatcc ggatccacca cgaagcccac atcggctccg gtttcagtca
     7441 ttttctgcga gatagcgttg agatgttcgg gcaacggttc cgggttgtgc ggaaattctc
     7501 cgttgggatt gcagtatagt tcaacaacgt caatgactcc aagtgctttg aggagtttcg
     7561 gtattacaat gccgcctact gaatttacac agtcgatcac cactttaaaa cgggccttac
     7621 ggatgacttc gggttgaaca agcgttagct tcaatatttc atcgatgtgt ttattttcgt
     7681 agtttagtat ttcagttatt ttcccaagag aatcaattgc ggcaaacgaa aaatccgatt
     7741 tatttgcaag ttccagtatc agaattcctt cttcagcggt taggaattct cctttgctgt
     7801 taagcatttt aagtgcattc cattggcgtg ggttgtggct ggcagtaatg ataataccac
     7861 catcggcttt ttcgagaata acagccattt cggttgttgg tgttgtggca aggcccaggt
     7921 caataacaga tattcccatt gatatgagtg tgttgcacac aaaattgtgc actaccggtc
     7981 ccgaaattct ggcatcgcgc ccgactacta tggttaagcc aactttcgcg gaattactgt
     8041 gttgcatcca ctgggcatat gctgcagtaa atttgcatat atcaacaggg gtgagtcctt
     8101 ctccctgaac accgcctatg gtacccctga tgcctgagat tgatttgatt agcgtcatga
     8161 tttatttatt taataaaatg caaatatata attcgtgaaa ttcagcaaag aaaaaaaaca
     8221 gatttttatg aataatcgca aaagcgtgaa aaataacctg aaattttatt tgttctttta
     8281 aaaaatacac tatttttgca acccaaatta taattaaata tttgcatcat gatagtgata
     8341 cctgtaaaag aaggcgaaaa catcgacaaa gcgctgaaac gttttaaacg taaattcgaa
     8401 aaaaccggag ttgtaaagga attacgtgta cgtcaggcat ttacaaaacc ttctattgaa
     8461 aaacgtcagc aaaaactgaa agccatctat attcagcatt tgcaggaaga ggttcagaaa
     8521 taagaaatta gcgtatttaa ttttgaaatg tctctgtaat gatgaaacat tacagaggca
     8581 tttttcgtaa aatagaatga tgttgattaa agatgatgca cataggtttg ttcctgaaat
     8641 acctgaaatt cgaaaagcga tactccgaaa acacaataag tgcctacaac aacgatttgt
     8701 tgcagttcaa tagctttatc gagtcggaaa cagcaggaat ttctgttgag aatgcttcac
     8761 gtaatataat tcgaagctgg atagttagtc tggcatcgga aaaaaaagat agcccccggt
     8821 caattcagcg gaaagtaacc agtcttcgca gtttttacaa atacctgcta cgggaaggag
     8881 ttattcagaa aaatccgact gtgggtattc acccgccaaa aaccgaaaag aagcttcctg
     8941 tatttctcac acaatcgcaa accgattttt ttattgatga agactgtttt ggcgacgatt
     9001 tcagtggagc acgcgatcgt gccataatag aaatgcttta caacacaggg atgcgtctgt
     9061 cggaagcagc gcaaatgaag cacatcgata ttgatttaaa atccaaccaa cttaaagtgt
     9121 taggaaagcg taataaggaa cgaattattc cgtttaataa ataccttggt gagataatta
     9181 taaagtatat taaccgaaaa aacgaaatgg ggataggtat ttcagcagaa gaccgttttt
     9241 ttgttaccga caagggaaag ccagtatatg tgaagttcat atatcacaag gtgcggcact
     9301 acattggtat ggtttctaca aataaaaaga aaagtccgca tgttcttcgc catacctttg
     9361 ccacacacat gttaaataac ggtgccgacc tgaatgcaat aaaagaatta ctgggtcatg
     9421 ccaatcttgc ggcaacacag gtttacaccc acagttcatt cgaaaaactg aaagaaattt
     9481 ataaacaagc ccatcctagg gcataaaaat caacaggagg aaaaatcatg aaagttacta
     9541 ttcattcctt gcattttgat gcagacaaga agttgcttga ttttattgat ggaaaaatta
     9601 caaaactgag tcagatatcc gatgaaataa tatcatcgga tgttgtttta cggattgaaa
     9661 actcggagac aaacgaaaat aaggttgcag agataaaact aaagataccc ggagcaaacg
     9721 aaatattcgc aaaaaaacaa agccgtacat ttgaggaatc cattgacagt gcaacaaatg
     9781 cacttcgccg gcagctacgc aagaccaaag aaaaacagcg tacgcaaaaa aaatgaaaaa
     9841 aataatttca tttatttgga tataatcaaa taataactac atttgcagac ctttttggaa
     9901 actgtccaaa acgttcttta atgtgttatt attgtgagta aaatgccaat gtagctcagt
     9961 tggtagagca gctgatttgt aatcagccgg tcggcggttc gagtccgtcc attggctcgt
    10021 gattactcta aaatggggag ataccaaagt ggccaactgg ggcagactgt aaatctgttg
    10081 gcgtacgcct tcgtaggttc gaatcctgct ctccccacgt taagaaagcg ggaatagctc
    10141 agttggtaga gcacaaccct tccaaggttg gggtcgcgag tccgagtctc gtttcccgct
    10201 cttaattttg ccaaagtagc tcaggggtag agcgtttcct tggtaaggaa gaggtcatgg
    10261 gttcaattcc catctttggc tcgaatattt tttttattgt ataacaaata taactagtaa
    10321 atttttaacc catggctaaa gaaaaattct caagaacgaa accgcacgtt aacatcggaa
    10381 ccattggtca cgttgaccat ggaaaaacca ctctgaccgc tgctataacc aaagcgctgg
    10441 caacagatgg tctggctgtt tacagggatt tcgattcgat tgacaacgct cctgaagaaa
    10501 aagaaagagg tattaccatt aataccgctc acgttgagta tgaaacaaaa aatcgtcact
    10561 atgcacacgt tgactgtccg ggtcacgccg actatgtaaa gaacatggtt accggtgctg
    10621 cccagatgga cggcgcaata ctcgttgttg ctgctaccga tggtccgatg ccacaaacac
    10681 gcgaacacat tttgctcgct cgtcaggtaa acgtaccgaa aatcgttgtt ttcctgaata
    10741 aagtggatat ggttgatgat gaagaattaa ttgaattggt tgaaatggaa gttcgcgaac
    10801 ttctagattt ctacgaattt gaaggtggca caggtccggt tattcgcgga tcagctctgg
    10861 gcgctctgaa ttcagaaccc aaatgggttg agaaagtata tgagctgatg gcagctgttg
    10921 atgaatggat tccgattcct ccgcgtgata tcgaaaaacc attcctgatg ccggttgaag
    10981 atgtgttctc aattacaggt cgtggtactg ttgccactgg tcgtattgaa accggtgttg
    11041 ttaacacagg tgatgaagtt cacattatcg gactgggtgc tgaaaaccgt aaaactgttg
    11101 ttactggtgt ggaaatgttc cgcaaaattc tcgacagagg agaagctggt gataatgttg
    11161 gtctgttgct tcgcggtatc gataaaaatg aaattaaaag gggtatggtt gttgccaagc
    11221 ccggtagcat caaaccgcat actaaggtga aagctgaggt ttatgtattg aaaaaagaag
    11281 aaggtggtcg tcacactcct ttccacaata actaccgtcc gcagttctat atcagaaccc
    11341 ttgatattac cggcgaaatt caattgccag aaggcgttga aatggttatg cccggcgata
    11401 atgtaaccat taccattaac cttatttacg aagttgcttg caacgttggt ttaaggtttg
    11461 ctatccgcga aggtggtcgc acagtgggtg ccggtcagat tattgaaatt attgaatgag
    11521 aataaagtag gttcagggtt ggctgcaaag ccaactctgt aaacttacgg gtgtagctca
    11581 attggtagag cgatggtctc caaaaccata ggctgtgggt tcgaagcctg ccacccgtgc
    11641 aaattataaa gtgtatggca aagatatcag catactttaa agaaacatac gatgagttgg
    11701 cccataaagt tacatggccg acttggagtg aattgcagac tagtgcaatt gttgttatgg
    11761 tggcttccgt aatcatagct atcgtggtgc tcgcaatgga ccttattttc cgtaatggtc
    11821 ttgagttcct atatggcttg ttttattaat aaatagaggc tggaggaaca ttatgggtga
    11881 tcttgaaaaa aaatggtatg tagttaaagc acttagcggc aaagaaaaaa aggtaaaaga
    11941 atacctcgaa aacgaagttg ctaaaaccaa actaacaaac caaattactc aggtgcttat
    12001 tccaaccgaa aaagtttacc aagttcggaa aggcaaaaaa ataagcaaag aaagaaattt
    12061 ctttccgggc tatattctga ttgaagcaat acttgatgga gaggtagagc atataatcag
    12121 agctgtcccc ggtgtgcttg gcttccttgg tgccaacgac ggtaaaccga ttcctttacg
    12181 accaaacgaa gtcgcccgta ttcttggaaa agttgatgaa ctgaccgaaa gctcagaaga
    12241 aatcaacgtt cccttctata ccggcgaagc ggttaaagtg attgatggtc cgtttaacgg
    12301 ttttaacgga attattgaag aagtgcatga agaaaagaaa aagctgaagg ttatggttaa
    12361 aatatttggc cgtaaaactc cgctcgaatt gagctttatg caagtagaaa aagagtaaaa
    12421 atcacttatt caattagtgt tatggcaaaa caaattgaga attttattaa attacaaatt
    12481 aaaggtggcg ctgcaaatcc ttcaccgccg gtaggacccg cacttggttc aaagggtgtg
    12541 aatattatgg agttctgcaa acagtttaac gccagaaccc aggatagagc cggaaaagta
    12601 ttaccggtca ttatcacggt gtattctgat aagtcgttcg actttatagt gaaaactccc
    12661 cctgtggccg tgcaactcat cgaaattgca aaacttaaaa aaggatcggc tgaacccaac
    12721 cgtaacaaag ttggctcagt ttcatgggaa gatgttaaag caatagcaac ggacaaaatg
    12781 cccgacctaa atgcttttac tattgaatct gccatgaaaa tggttgccgg tactgcaagg
    12841 agtatgggaa ttactgttag tggagagtca ccatttaaaa attaaactta agcacttttt
    12901 ttaaaatggc aagaataagt aaaaacaaaa aagaagcttt atcgaaactt gacaaaacca
    12961 gaaagtacga tatcaaagaa gctgcaaaac ttgtaaagga aattacctat acaaaattcg
    13021 atgcttccgt tgatattgac gtgaaactgg gtgttgaccc gcgtaaagcc aatcaaatgg
    13081 ttcgcggtat aactacactg cctcatggaa ccggtaagca aatcagggtg cttgtgttgt
    13141 gcaatcccga caaagagcag gaggccaaag aagcaggagc cgattatgtc ggacttgatg
    13201 attacatcga aaagataacc aacggatgga ctgatgtgga tatcataata acacaaccta
    13261 tgattatggg taaaattggt aaactcggac gcgtactggg tccgcgtggt ctgatgccca
    13321 atccgaaatc aggtactgtt actcaggaaa tcggaaaagc tgttcttgat gtgaaaaaag
    13381 gtaaaataga ttttaaagtc gataagtatg gtataatcca tgctggaatt ggtaaagttt
    13441 cgttcgagac cgaaaaaatc gtagataatg ccagcgaact ggttcagaca attgtaaaac
    13501 tgaaacccag cacagctaaa ggaacataca ttcaaagtat tttcctgtcg agcactatga
    13561 gcccaggtat cgaaatagac ccaaaatcta tagttcagta acttttagaa ttaagaatgt
    13621 atgaaacgtg aagaaaaaga tgtcctgatt gagcaactga aagaacaaat cggtatgtat
    13681 agtcactttt atctgactga tgcagccgaa ttgaatgccc aggaagtatc agacctcagg
    13741 cgcaaatgtt ttgaatccga cataaaaatg gtggttgtga aaaacacctt gtttaagctt
    13801 gcactggaga gggcaggcaa acattacgaa gagttatacc ctgcgttgca gaataacaca
    13861 gctgttttgt tttgcaatac tgcctccact ccgggaaaac ttattaagga attcagaaaa
    13921 aaccatgaaa agcccgtatt taaagcagct tatgccgagg aaatggtatt tatcggagaa
    13981 gaccagttag atgcacttgc aacaatcaaa acgaaagatg aattgattgg cgatttgatc
    14041 ttcttgctgc aatctccaat gaagcagctt ctaagtcagt tgaattcagg taaacacatt
    14101 gtagccggag ttgtaaaaac actggctgaa agagagtaat aacaaaagtg attcattatt
    14161 aaaattataa aaaaatggca gatctgaaaa aattagctga agaattagtt aatcttagtg
    14221 taaaagacgt aaatgagttg gcaagtattc ttaaaaatga atatggtatt gaacccgctg
    14281 ctgcagctgc agtagttgct gctcccactg ccggtggtgc cgctgccgca gttgttgaag
    14341 aacaaaccga gtttgatgtt attttgaaag ccgccggtgc tgcaaaatta caggttgtta
    14401 aacttgtaaa agaacttaca ggcctggggc tcaaagaagc aaaagacctt gttgacgctg
    14461 caccaaaacc ggtgaaagaa aaagtatcga aagacgaagc cagttcatta aaagctaaac
    14521 tcgaagaagc aggagctgaa gttgaggtta aataaattgg cttttggcat ttgaatttta
    14581 ggtttaaaac ccatatacgg gttttaaacc tttcgttgtt attgatttag ttctttatta
    14641 aaatattatc aatggctatt aaaagaaatg aacgaatcaa ttttagttct aatcaaaatc
    14701 agctggaata tcctgatttc ttagaaattc aattgaaatc attccgcgag tttttccaga
    14761 tagaatcaac agctgagcag cgtaaaaacg aattactttt tcaggttttt caagaaaact
    14821 ttcctatttc ggatacccga aataattttg tgcttgaatt tctcgattat tcggtcgacc
    14881 tgccccgtta ttctattcag gagtgtgttg aaagaggttt aacctattgt gttcccctaa
    14941 aggcaaaatt aaaattatat tgcaccgacc ccgatcacga agattttgaa acaaccatac
    15001 aggatgtgtg gctaggcacg gtgccatata tgacaccttt cggaacattt gtgataaacg
    15061 gtgccgaacg agttgtagtt tcgcagcttc accgctcacc cggtgtgttt ttcggtcaaa
    15121 gcattcatac caatggtaca aagttgtatt cagccagaat aattcctttc aagggctcat
    15181 ggattgaatt tgcaactgat ataaattcgg tgatgtatgc ctatatcgac agaaaaaaga
    15241 aattaccagt tactacatta ctcagggcaa ttggttttga aagtgacaaa gatattcttg
    15301 aaattttcga ccttgccgaa gaaattaaag ttagtaaaac tgcactaaaa aaagttgttg
    15361 gacgtcgcct tgctgccaga gtactgagaa cctggacaga agatttcgtt gacgaagata
    15421 caggtgaagt agtttcaata gagcgtaacg aagttattgt tgaccgcgaa gtggttctta
    15481 ccaacgaaca tgttgattta atcgtggacg ccggggccaa aatggttctt ctccataaag
    15541 aaaatcagaa aacatccgac tacgcaatta tttacaatac tttgcaaaaa gacccctgca
    15601 attcggaaaa cgaagcagta gtgtacattt atcaccagct ccgcaacagt gaaccccccg
    15661 atgaagctac cgctcgtgat gttattgata agctgttttt ctccgataaa agatatgatc
    15721 taggtgaagt aggacggtac aggctgaata aaaaactcaa cctagaaatc cctatggaaa
    15781 caaaagtgct caccagagac gatataatcg ctattataaa atacctcatt aagcttatca
    15841 attcaaaagc cgatatcgat gatatcgacc atttgagtaa tcgtcgcgtt aggacagtag
    15901 gtgagcagct tgccagccag tttggcgttg gccttgctcg tatggcaaga acaatacgcg
    15961 aaagaatgaa tgttcgcgat aacgaagttt ttcatcctat cgacctgatt aattcaaaaa
    16021 ccctttcttc ggtaatcaac tcatttttcg ggacaaacca attgtcgcag ttcatggacc
    16081 aaaccaatcc acttgctgag atgacacaca aacgccgtat gtcagcattg ggaccaggtg
    16141 ggctttcgag agaaagagca gggttcgaag tccgcgacgt acattataca cactatggac
    16201 gtctttgccc gattgaaaca cccgaaggtc cgaatatcgg tctgatttca tctctgtgcg
    16261 tgtttgccaa aatcaataaa ttgggattca tcgaaacacc atatcgtcag gttgaaaacg
    16321 ggaaagttca gattgatggt gatgttctgt ttttaagcgc agaagaagaa gaaagaaaaa
    16381 ttattgcaca ggcaaatgca aagcttgatc ctgatggaac attttccaac accagagtaa
    16441 aagcccgatt ggaagcggat tatccattgg cggaaaaaga aagcgtacat ctgatggacg
    16501 tagctccaaa tcagattgct tcgatagcag catccttgat accatttttg gaacacgacg
    16561 acgcaaaccg tgctctgatg ggctcaaaca tgatgaggca ggctgtcccg ttgttaaatc
    16621 ccgaagctcc catcgtggga accggaattg aatatgaagt agcccgtgac tcaagaatac
    16681 ttgttattgc cgaaggaaat ggcgtagttg agtatgttga tgcaaaggaa ataattgtac
    16741 gatacgacct gaccgacgag gaaagatttg tttcgttcca tgatgacgtt actgcttaca
    16801 atcttcagaa atttcagaaa accaaccaaa acacttgtgt aaatcagaaa cccttggtta
    16861 aaaaaggaga gagggttgtt aaaggtcaga tactttccga aggttatgct acccgaaatg
    16921 gggaactcgc gttgggaaga aatcttaaag tggcatttat gccatggaaa ggctacaact
    16981 ttgaggatgc aattgtgatc tcagaaaagg tagtccgtga tgactatttc acgtcaattc
    17041 atattgaaga acatcagctg gaggttcgag atacgaaacg tggtcttgag gaactgactt
    17101 ccgacattcc gaacgtaagc gaaattgcta ctaaagatct tgatgagaat ggtattatcc
    17161 gtattggtgc atatgtgaaa ccgggggata tacttattgg taaaattact ccaaagggtg
    17221 aatcagaccc atcgccggaa gaaaagttgc tgcgtgcaat ttttggtgaa aaggcaggtg
    17281 atgttaaaga tgcttcactt aaagcatctc cgtcaaccaa tggagtcgtt attaataaga
    17341 agttgttctc aaaaactatt aaagaccgaa aaacaaagaa tcaggataaa gtgcttctcg
    17401 atgctataga gagtgagttc aataaaaaag ccgacgctct gaaagaagcc cttgttgata
    17461 aactgttctt tattgtaaac ggtaaaacct cgcagggagt gaaagacaac ctgaataacg
    17521 atgtaatacc taaaggtaat aaatttacac agaagctgct gtttgaggtg gattacatca
    17581 atgttaaccc ctgcaaatgg accaccgata aaaagaaaaa cgatattatt gaaaagctcc
    17641 ttttcaatta ctcgatagaa tataagaaaa tcgaaggaga ggcccgtcgt aaacgactga
    17701 acatctcgat tggcgatgaa ctacctgcag ggattgtaca gcttgcaaaa gtgtatcttg
    17761 ctagtaaacg taagatcacg gttggtgata aacttgccgg acggcatggg aacaaaggta
    17821 ttgttgctaa aatcgttcgt caggaagata tgccattcct cgaagatgga actcctgttg
    17881 atattgtgtt gaatcctttg ggtgtgcctt cgcgtatgaa ccttggacag atttacgaaa
    17941 ctgttcttgg atgggcaggt gttaatctgg ggctgaaatt tgccacaccg attttcgatg
    18001 gggcaactat tgaacagata aacgattaca ctgagaaagc cggaatcccg cgatttggca
    18061 aatgctacct ctatgatggt ggtaccggaa tgaaattcga tcagcctgcc acagttggcg
    18121 taatatatat gctgaagctt gggcacatgg ttgaagacaa aatgcacgcc cgttccatag
    18181 gtccatattc gctgattaca cagcaacccc ttggcggtaa ggcacagttc ggaggacaac
    18241 gctttggtga aatggaagtt tgggctcttg aagcatttgg ggcatccaat attttaagag
    18301 aaattcttac cattaaatcg gatgatgtta tcggcagggc taaaacttac gaagctattg
    18361 ttaagggtga tcccatgccg gacccaggtg ttcctgaatc gctgaacgtt ttgctccacg
    18421 aaatgcgtgg ccttggatta aatgttgaat tggaatagta ttattaaatt gaatatataa
    18481 tttattatgg ctttcagaaa agacttaaaa gtaaaatcga acttcacaaa aataagtatc
    18541 agccttgctt cgcccgaaga tattctcgac aggtcgagcg gtgaagtcac aaaacctgaa
    18601 accatcaatt accgcaccta taagcctgaa cgcgatggtt tgttttgcga aagaatattc
    18661 ggtcctgtaa aggattatga atgccattgc ggtaaataca aaagaatacg atacaaaggt
    18721 attgtatgtg acagatgcgg tgttgaagtt accgagaaga aagtgcgccg tgaaagaatg
    18781 gggcatataa aactagaagt gccggttgca cacatctggt atttcaaaac cctgccaaat
    18841 aaaattggtt acctgctggg aattccatca aagaagatgg actctattgt gtattacgaa
    18901 cgttatgttg taatacaacc aggtattaaa aatgccgatg gagtaaatca gctcgacttt
    18961 ctttctgaag aagaatatct tgcaattctg gatactttgc ccaaggaaaa ccagatgctc
    19021 cctgatgatg accccaataa gttcatcgca aaaatgggtg cagaagctat ttttgatctg
    19081 cttacaaaaa taaatctcga cgaattatcg tacgatttac gcgataaagc cggtaaagaa
    19141 acgtctcttc aacgtaaaag cgaagctctt aaacgcttga gcgttgtaga agccttccgt
    19201 gaatcggaag gaagaaataa accggaatgg atgattgtaa aggttgtacc ggtaatccct
    19261 ccggaactaa gaccattggt tccgcttgat ggtggcagat ttgcaacttc cgacctgaat
    19321 gatttatatc gccgcgtaat tattagaaat aaccgcctca aaaggcttct cgaaattaaa
    19381 gctccagaag ttattctgcg taacgaaaaa cgtatgttgc aggaagccgt tgattcactt
    19441 tttgataatt ccagaaaatc aaacgccgtt aaaaccgaag ctaacagacc tctgaaatcg
    19501 ctaagcgaca gcttgaaagg gaagcaggga cgtttccgtc agaatctgct ggggaaacgt
    19561 gtcgattatt ctgcacgatc ggttattgtg gttggtcctg agctgaaaat gcatgagtgt
    19621 ggtctgccaa aagatatggc agctgagtta tacaaaccgt ttattatcag gaaattgatt
    19681 gaacgtggta ttgttaaaac cgtaaaatcg gcaaaaaaaa ttgttgaacg tcgtgactca
    19741 gtaatttggg acattctcga aaacgttctc aaagggcatc ctgttatgct taaccgggcc
    19801 ccaacactgc accgtttggg aattcaggct tttcaaccca aacttattga aggaaaggct
    19861 attcaattgc acccattagt ctgcactgct ttcaatgctg acttcgacgg tgaccagatg
    19921 gcagttcacc tgccacttgg aaacgaagct atcatcgaag cgcaactgct gatgttggct
    19981 tcacataata tacttaaccc tgcaaacggt gctccaatta ctgttccatc tcaggacatg
    20041 gtacttggat tgtattatat caccaaaaaa cgcaggggcg ctttgggaga agggctgacg
    20101 ttctatggac ctaaagaagt aattattgca tacaacgagg caaaacttgg cttgcatgca
    20161 atcattaaag ttaaggttga tgatattgat aaagatggca actacatcaa tcatttgatt
    20221 gaaacaacaa ccggacgcgt tatttttaac cagtcagttc ccagagaagt tggctatttg
    20281 aacgaagtac tgacaaaaaa atccttacgt tcgattattg gaacagtgct caaaaaggca
    20341 ggaacagctg ccactgcaaa attcctcgac gatattaaag atcttggttt cagcatggct
    20401 ttcaaaggag gtctgtcgtt taacctcgat aatgtgatag tacctgaaag caaacagaaa
    20461 atgattgaag aaggctatcg tgctgttgag gaaatacgat ctaattacga atcagggttt
    20521 attaccaata atgaaaggta caatcaggtt atcgacgtat ggactcacgt aaatgcaaac
    20581 cttgcaaaag aagttatgaa gcagttgtcg gaagacaatc agggctttaa ttcagtatat
    20641 atgatgcttg actccggtgc ccgcggttct aacgaacaga ttaggcagtt gtgtggtatg
    20701 aggggtttga tgggtaaacc gcagaaatca atctcaaccg gagctgaaat tattgaaaac
    20761 ccgattcttt ccaacttcaa agaaggcttg tcgattctgg agtattttat ttcaactcac
    20821 ggcgcacgta agggtcttgc cgacacagct cttaaaactg cagacgctgg ttacctcaca
    20881 cgacgacttg ttgacgtggc tcaggacatt gtaatcaacg aagaagactg cggaacgctg
    20941 agaggacttg tggcaaccac aatcaagaaa aacgaagaag tggttgaaac actctatgaa
    21001 agaatactgg gtcgtacaac agttcatgat gtatatcatc ctcagacggg agaacttatt
    21061 gttgctgccg ggcaggaaat cacagaagac gtagccgata ttattgattc atctcctatc
    21121 gaagaggttg aaatcaggtc ggtgttaact tgcgaatcga aacgaggtgt ttgtgcaaaa
    21181 tgctatggcc gaaatctggc aactggacga atggtgcaaa aaggtgaagc tgttggtgtg
    21241 atagcggctc aatcaattgg tgagccggga acacagctta cccttcgtac tttccacgtc
    21301 ggcggggttg caggtaacat tgcaactgaa tcaactattt ttgtgaaata cgaaggtatt
    21361 gttgagattg aggatctcag aacagttgaa tacgttaatg aacaaggtga aaaatatgat
    21421 attgttattg gtcgtatggg cgaaatgaga ctggttgata aaaacacagg catcattatt
    21481 atgactaaca atattcctta tgggtcgaaa ctattcgtaa aaccaggtca gtttgttgag
    21541 aaaaacactg tggtttgcag ctgggatccg ttcaactcag taatcattac tgaagttgcc
    21601 ggtaaagttg actttgaaaa tgttattgaa gggattactt atcgagaaga agcggatgat
    21661 cagacaggct atcgcgaaaa agtaattatc gaaaccagag ataagtcgaa aataccagtt
    21721 gtaaagatta ttaccggtaa aaacgaagtc ctgaaaactt ataacctccc tgtgggtgca
    21781 catatcattg ttcaggagaa cgaaaaagta gtcgcaggta aaattcttgt taagataccg
    21841 agagttgttg gtaaaggtgg tgacatcacc ggtggtttgc cacgtgttac cgaacttttc
    21901 gaagcacgaa atccgtcgaa cccggcagtt gtttgtgaaa tcaatggtca ggtagaattc
    21961 ggaaaaatta aacgtggtaa cagggaaatc attatccgtt caaaggaaaa tgaggagaaa
    22021 cgctatctgg taccattatc aaaacaaatt ctcgtacaag acagcgattt tgttcgtgcc
    22081 ggaacaccat tgtcggaagg agctattacg ccaatcgata tactgaatat taaaggaccc
    22141 acaatggttc aggaatatat tgtaaatgag gttcaggaag tttaccgtat gcagggtgtg
    22201 aaaattaacg ataaacattt cgaagtaatt gttcggcaaa tgatgcgaaa agtcgaaata
    22261 gaagacccgg gtgatacccg tttcctcgag aagcaaattg ttgataaatg ggaattccgg
    22321 gaagttaacg acgctatctt cgataaaaaa gtggttattg agcccggtga ttcatctact
    22381 gcgaaagctg gacagattat ttccgccaga aaactgcgcg atgagaattc tattttgaaa
    22441 cgtaaagacc ttcagccaat aatggcccgc gatgccgttc cggcaacttc aagccaagtg
    22501 ttgcagggta ttacaagagc tgcattgcaa acaaagagct tcatgtcagc cgcttcattc
    22561 caggaaacaa caaaggttct gaatgaggct gctattagcg gaaaagtaga tcctctcgat
    22621 ggattgaaag aaaatgtaat tgtcggacac ctgattcctg ccggaacagg attgcgcgaa
    22681 tacagtacat atttggttgg caacaaagaa gaaatggctc aggtcagtga gaaatacgaa
    22741 cacgtcaatg aataagaaat atggaagata aaaaccttaa acaaaatcag atcagcatcg
    22801 aacttaagga agatgtcgct cagggtacct actcgaatct cgctgtgata acacatagca
    22861 gttcagagtt tgtaatcgat ttcgtaagga ttatgcccgg tgttccgaaa gctcaggtga
    22921 aatcaagagt cattctcact ccggagcatg ccaaaagact aatgatggct ttaaaggaca
    22981 atattcaaaa atacgagcaa atgaacgggc ctattaaagt aaatgaatcg agtcagggac
    23041 ctgtaattcc aatgacattc ggaggaccta cagctcaggc ctgaaattgt atatgaaatt
    23101 gaaaaaggct gcctttaaca atcgcagcct ttttttattc taatgtaaca ttaagcccct
    23161 tctcaaccag agagtccttc attggcttca aaatgtcgaa ttttcctttc ttaatttcac
    23221 actttccttt ataatgcgtg agaaaagcac attgctcagc ttgaaggtca tcgtgatcgc
    23281 aaatggtaac cagacattca ataacaaaat caaatgtgtt gaaatcgtcg ttatgcaaaa
    23341 caagaatgtt atccagagac aaattctcgc tcggattgcc tgaattttgt atttgtgtat
    23401 ccttttttgt catactgcaa atatacgcaa aaaactaatc atttatttca cgtaatggta
    23461 tttgtttccc gtttttataa gcaacaataa aggcatctgt tattcccgct tcaactgctg
    23521 ttatacgcac ttcgttagca gtggcttttg attgtagagg accaatacga tatatgataa
    23581 gattgtttct ttgaacagtg gtaatgttgt tagttccagc tatatttttg aacatgttgt
    23641 aaatttctgt attcacttca gaacgataag cacctatctg tacataaatt tcaatgacaa
    23701 aactctcagt gttaatggcc ggatttggtg tatttgtttt atgaacagca attgttgcag
    23761 tatcggtgga gccggatggc tgtgaggcaa ttagctgaag agcttcgtta accggtatcc
    23821 ttaccccgtt tcgataagcg gtaataaatg catcgtttgc ccctgtagct cgtattatgt
    23881 ttcgggcatt ggcagcatcg ttcattgaat agaattttcc gctcaggtgc ctgatataac
    23941 cattgtctaa aaattccaga aaatcagcac ttaacccgaa aatctcaccg ggtagtaaaa
    24001 tcctttttgc tgcagcaaat tgcacacaga gaaaaacccc ttctacggct tttacatcta
    24061 ttatgccata ttgtgagttc gctttttcaa cggagtctgt tacattttgg tttgtcgctt
    24121 gttgctggtc atttacagca ataatctcgt tctttgatac tttttgttgt gcaaggtatt
    24181 ggttcgcttc aaatatggta atccgtttcc cattcaagta agcaacaata aaagcatcgt
    24241 tgaagccaac atttttaaca ccgggaagcg cacgggcagc cgtttcgaaa ttgggataat
    24301 cgccggtcat ataccgtatc agctgtctat cagggacttt ctctgtgaac agagggctta
    24361 ggccttttgt gtattcatct ccaacaattc ggttatatgc agcaaattgg attctgaaaa
    24421 caagaacatt ttctgatgca ggggtattat ttgtattatt accattaaga accgtgtttt
    24481 gttgaacagt atcagcagca gggctttcaa gtatcatgat tgcctcactt cgttgcgaaa
    24541 gtgcctcttc atctaaggca tttgcctgag ccagcaggtc gcttttcgaa ttaattatat
    24601 cattgctctg catagctgct atccgcaacg aatcggcttg ttgttccttg cgccttgcat
    24661 cctttgtcag actatcagcc tgctgtttta aagcagggga atctgttttt aatttcaatc
    24721 gcttttcttc aagtttcgaa atggaataat tcagaatatc gtcatttgtt gctaagtact
    24781 tttccacctt ttggcttctg tcggtaagta gactgtcacg ttcggcaata agtttggttt
    24841 tgtccgtagc gttactggtt tttgagatat agtcctgaac aagcaggatt tcttcgtcaa
    24901 ttgattttat ttgcaaccgt aattcttcac gttcctttgt taatttatcg attacttctc
    24961 tatcagttgt attgttcagg aacttgtttg catcaatttc attttcaggc aggaggaata
    25021 ggctttcttt gcttataggc cggtatgtca taacaatgct atccctaatt ggattctcaa
    25081 ttaaataact gtagttggaa ttaatcagct gtgcggattg aatatcgttg ttggttgaaa
    25141 atttaatcgc ttgattttgt aaatcgattg cggcagtagt tagcgaatct gcatgttgtt
    25201 gcagttgatt tttctcattc tctgtaaacc tgttattgtt atcgctaata ttatcccgaa
    25261 tcttggtagc ttcattcatc aaactgtccg aaacaataat tgctttttct atagccgaat
    25321 ttatttcggt gctcgttgta gaaattgtgt cgagcaagtt acggtttaca ttaaacgtgt
    25381 cgttccatgc agcttctttt tgagcatacc gtacactgtc ttcgcgcgca atagcaagct
    25441 gtttatcaac ttcaattaac tcaattgctt tgttctggta tgccagtcct agggtttcga
    25501 gtgaatcggc ccgggccaca cgggcttcaa tttcattgtt tgagtaggtc tgaaaattgt
    25561 cggatagctc ctttcgaatt gtagcagcct cattcatagc actatcagcc cgggttacta
    25621 ttgatttgat ttccgggttg ttattaataa tatgcatatt cgaagtaagt gttctttcgc
    25681 tattctgcat gtaatttttt tgagcctcgg aaggaacagt caggttattg cgctgatttt
    25741 caaaactagt ggcctggcgt tgcacgtttt cggcaaccca attatccgtt ttcagcattt
    25801 tcctgccaat ttcttcgatg cggctggtaa ttgttttgtt ttcaggttga gtattgtttg
    25861 ttttttccga aattaactgc tgttgttttg caagaatgct gtcgccaaaa actacaattt
    25921 cctgaagcag gtagaagtaa ttggtttcgc tccggtatgc tttatcgctg atggctttgc
    25981 tgtaacttag ataatcatct tcaatttcga ggtacaactt tttacggtca gtgtaattgg
    26041 ctttatttgc ttcaataaaa tctctgtttt ttctgatttt ctgttgctgt aaatcaacga
    26101 gcgtactgta ggctgagtcc acaataatct cctgctcatt cctgagcgaa tcaatttcat
    26161 tgattatacg gttttgctcc gatggaatgt ctgttttttc atattccgtt tgtaacgaag
    26221 caatggaagt atttatgttt tctgctttta ttagcaaaga gtcgatttgc tgcatcacaa
    26281 tctcttcttc gttggttgac tgaaggttgg tatttgattg tggcaattca tttctaattt
    26341 cgatgtattc cgatacaatc gtgtctatgg ttaaattact ggtaacgaag ctgtattcag
    26401 gttctttaac tgatgtatag aagttggtgt ctatggcaat ttcgaaagcc tttttatatt
    26461 cttgcacggc ctgactctcg agctgggcag cttcaattat caattcattt ttcaaactgt
    26521 cgttggttgt ttcttctgca gcaatcagca ggtttgctgc ctggttatgc agtgctattg
    26581 ctgaattacg acggtctaaa gcttcagggg tgttcgcctt cgaaatgcta tggtcacttg
    26641 tttccctgaa tgatttatca aaaattagat attcttcaag gttcttattt agcagttgct
    26701 cagcagcctg tgcatgcagg tcgcgggttt ccttaaaaat ttgagttgca taatcctgca
    26761 agcttatttt tgtattgtca tcagatgtat tcctgatggc agaatttatt tcatcaagct
    26821 cgttgtaacc ggcaatagtt ttatcccaga tgctgtcgtt gttttggttg ctatatgtgt
    26881 tttcaataat ctggatctgt tgcttggttt cattgataat gctatcaatt gataaggagt
    26941 caggttgata aaccaataaa gtgtcagaat ttatcgtttc gttttggttt gtgttattaa
    27001 aattattctg attatttgcc gtaaggttgt tgtgtgtaat ttcgaatgct tttgcttcat
    27061 atattattgc ttctttcagg taataatcct gcaaggccgg caatttgtca tccgcttccg
    27121 ctgccattgc atataatgaa tcagcagttg taaggtaatc ttctgcgagt attacatcat
    27181 ttgagagtcc cttttctttt tgaatagcaa tctggctttc ggttttatct ctgatgtcat
    27241 caacattttc attcgaaaca ttattaacag caatcgtgtt gtttactaca gtaatttcat
    27301 cgttgatgtg ggtataaact tcattaagtt cagcaagggt gttattataa ttgttcagga
    27361 gttcgttcct cttttcgata ttggtttccg actcaaacat tttttgggta tgttcaagtt
    27421 ctgctccgag agaatcggct gttgcaaggt aagtgtttac aaggctttca tcaaaaagag
    27481 ggctttcggt gattatatgt attttctgga tattattttt ctcgtgcagg gttttctcgt
    27541 gcaacatatt gttttcagcc aatatcctat tatttgccag agttgaattg tcgaaaaaca
    27601 cagaatcgag cacctggttc tgcattacag caagatcttt caggtttcgc tgatcgcggc
    27661 gagcaatata tttttctgcc ttattcagca caatttcagc actgagccct gttttagggt
    27721 aattgacatt cggttcattt actaatgatg ctgcaattgc ctgttctttt tcgagctgcc
    27781 gggtttcttt tataaggaat tcctgttgga gaaaccgttc tttgtaatct acaaccaggc
    27841 gattccgctc cgttttaagc atttcaattt gtttccttgc tgcctgttgt ttttcagggt
    27901 cattcgtaaa cagcaagctg tcgttcaaat ccatgatttg cgaatcaatt tcgttaattc
    27961 tcctctcaac tgactgtaga tagtcgtggt ttgttctgat ttgattgttt ttggcttcaa
    28021 tttccttttg caggttttcc agaaaatctg accccggatt aacagtaagt tctttcttga
    28081 cttcggtatt tataatatcc agactttttt cgttggtagg gttttcggta ttatgatttt
    28141 cgatttcagt tatttgggta ttgtaatagt ctgatttctg cttcatttca ttaatggtaa
    28201 gctcgtaatt gtcagatagg tcctttaaaa cagcaaggct gtcggtgtag acatatattt
    28261 tcgcgtcaat atttgaaatt tgcttgtttt tttcttcggc agggatgttt ttaccagcaa
    28321 ttagttgttt ctccacctcg agcctatcga tttcatccat gtactgctga atttgcgaca
    28381 gggtcgcttc gtttttaatt gtgtaaaatt cagctctttt ttgtaaacta tcaatgttgg
    28441 tgtttgcttc atggagttta tcagtagtgg cggtatcatt tgatggggtg ttaatgtcag
    28501 ggatttgatt attgaactca gaagcagtat cgttattcaa ggcaatcagc gcctgcgacc
    28561 ttttttcgaa ttcttttact gctatagggt taatttgcaa atgtgaagtc tttcttacgt
    28621 catcgatgtt tgcaatctgc cgttgggccg gatttttttc aatcaatatc tgatacactc
    28681 cgattcttcc ggcttcagcc tctctggttg atgcaaaaaa ggcagtaagg tccctatcgt
    28741 ctgttatgta cataatatct tcaagtggag tgttgatagg aaaatcgagg ttctccggtt
    28801 ccgaccattt atccgattcg cggttatagg caactctgaa aatatcgtaa ccgcccatgc
    28861 tgttgtgacc ttttgaacta aaatacaaga attctccatt agggtgaaaa aacggaaatg
    28921 actcatcttc aacggaattt acaattttcg gcagcttttt gggagcactc catgcatcac
    28981 cttctttaac taccatatac aagtcaagat tacttttctt ttctgtacca tagctgctga
    29041 aaaatgcaat attgtacaac tttgaaacat acatcaaacc tgaatagttt cgtttcttgt
    29101 cgtttttagt tttaaaggct tcggggataa caactatttc tccacttctg gagtttgagt
    29161 tgtaggagta gtaaaaattg tcagttttta cgtttttcga ttcaacaact tgcaaatcat
    29221 agctataccg aatcaggtca attccgtttt gcgccatttt aatctgccgg tccaccggca
    29281 gcagctttat gtcctttccc gttgcccttt gctgataatc ttcgtagtaa cgaatagcgt
    29341 tgtcgaacat ataacgcaga tgataggcca ttccaagaaa gtagtttgcc tgatacatac
    29401 ccccgtctaa ggcatattcc aaataatcta tgctttttgt aaattcagta ttcaaacaaa
    29461 caagacatgc cccgtaggca taattgaaat atatttctct tggatatagg ctcagaagct
    29521 gcgagaatcg ttcttttgcc gaaagatacg attcactctc aaattcataa acagcttgtt
    29581 cgaacacctt ttgttctccg ggtagtagtt ttacactcaa ttcctgtgaa aaaccactga
    29641 aacgaaaaat tatcagggtg aagatggaaa agagccatac tattttatat acctgcgtca
    29701 atttcggtaa gcctttaaca aaagcaatga tggttatgtt tgtgcaattc cataaaacct
    29761 ataaagatac ataaaaatag tgtcagccta acaaaagcct ctgtttttga tatttgggat
    29821 gtaaaatcgc acgattgtta ataaactttt attttcaaac ccctgattct gcactgaaaa
    29881 aaatatattt gtacaaagaa atgtatttat gcaaaaatta aagcttggcg ataaagctcc
    29941 tgattttaag ggaattacac agtcaggaaa gattgttgcg ctatcagact atagtgggaa
    30001 aaagctgata ttatattttt atccgaaaga caataccccc ggttgtactg cagaagcctg
    30061 cgaccttcgg gataactacg aaatgtggca gcataaaggg tatcaggttt taggtgttag
    30121 tgccgacagc actaaaagcc atcaggggtt tgccgaaaaa tataatctgc ctttcgacct
    30181 gatttcagac accgagaagc aaattctcaa agcctatgga gcctgggggg aaaaaaaact
    30241 ttatggtaaa agctatgaag gtgtactgag aacaacttac attatatcgg agaatggtat
    30301 tattgaacaa atatttgaga aagttgatac aaaaaatcat agtcagcaaa taattaaaaa
    30361 cctttaaatt atatggcaaa gaacacaaac gaagatatta agcttgaaaa actgaaagcg
    30421 ttacagctca cgctgggtaa aatagaaaaa gactacggta aaggagccat catgaagctt
    30481 ggcgataagg ttgtggaaaa tgtacccatt ataccttctg gatcggtaac attgaactat
    30541 gcattgggag taggagggta tccccgtgga agagtaattg aaatatacgg ccctgaatca
    30601 tcgggaaaaa caaccttagc gattcatgct attgctgaag cacagaaaag tggtggcatt
    30661 gctgccatta ttgatgcaga gcatgctttc gatagaacct atgctgagaa actgggtgtt
    30721 gatacacaga atctgtttat atcacaaccc gataacggag agcaagccct cgaaattgct
    30781 gataatttga tacgttcggg cgctatagat attatagtta tcgactcagt tgctgcactt
    30841 accccaaaag cagagatcga aggtgaaatg ggcgacagta aaatggggct gcaggcccgt
    30901 ttaatgtcgc aggctttacg aaaacttact gcaaatatca gcaaaacgca gacctgctgt
    30961 atatttatca atcaactacg cgataaaatt ggagtgatgt ttggcagccc cgaaactact
    31021 acgggtggaa acgcacttaa attctatgcc agcatccgcc tcgatatccg gagaatttcc
    31081 cagattaaag agagcgaaga tgtaacgggt agtcgtacca gagtgaaagt agttaaaaac
    31141 aaaatggctc cgccgtttaa gaaagcagaa ttcgacattg tgtacggtga aggcataagt
    31201 aagataggtg agattattga tttaagcgtt gattatgaaa taatcaagaa aagcggatcg
    31261 tggttcagct atggtgacac caaacttgga cagggacgtg atgctgtgaa acagctactt
    31321 cttgataatc cggaactcgc cgaagaactc gagaagaagg ttgttgcaag gctcgaagcc
    31381 aactaataat tctttgggtt gccaatgact aagctccgga atatttttgt cgtatctggg
    31441 attttaatat cttatgttct catattcctt gcactcaaca gctgttcatg taattcggga
    31501 aaacaaaaag ctttaaccgt tgcaaacgat tctgttgatt ctataaaaat attgtctgct
    31561 caaatcagaa gtaacccaag acagcattca ctcttttata aaagagcctt gctgaattac
    31621 gggaatggta accttcagga agccgtaaac gatatcgaaa ttgccttaaa actcgattca
    31681 atcaatccgg tttacctgca cacagctgct gactattttc tgttgcttgg aaaatcggaa
    31741 cgctcgagag atgcattgtt gctatgtata aagcaccatc cgcaggacaa agaagcccac
    31801 ttcaagcttg ctcagttgta ttattatgtg aaacaatacg atgatgcaat caaagaaata
    31861 gcctttattg agggcatgaa ccagcagagc gataaaacgt attttctgaa ggcgctgatt
    31921 tttaaagaaa ataatatgac ggtaaaagct gttgaaagcc ttctttctgt gcttgaatac
    31981 aatccgaaac atgctgaagc atataacatg attggtatga tttattacga ggctgataat
    32041 aaactggccg tcgattactt taatactgcc gtattgcttt ttcccgataa tctgcaaata
    32101 cttctcaatg ctggtctggt gtacgaaaaa tttggattaa acgataaaac acgtcaggtt
    32161 tttgaaaagg caatcagtgt agatagtacc gattatcagg ccaattataa tatgggatat
    32221 tatttattgg tgtatggcga tgatgtcaaa gctgctatag gttatttttc aaaggctgtt
    32281 gagatcgacc cgaaggctgt gaatgcatat tataacagag gactggccta tgagctctcc
    32341 gggaatgaga cagaagcaag aaacgactat aacaaagcgc ttgaaattgc accgaattac
    32401 gaccttgctg tggaagcact gaataagcta gacaaaagat aaaaattaat tataaaatct
    32461 aatattatga ggtcaattgc attttccgtg ttattgtttt cgatttttct ggtttttgaa
    32521 tcctgtattc agaatccggt cgaaaaatct gaaaaaactc cagtaaatgt ttcccttact
    32581 gatgctgaaa aaaaagcagc tgtattaaca ccggaaataa tgtggaaatt tggcaggatt
    32641 ggtgaaatgc agctatcgca tgatggcaaa gaggtactgt atacagtaac tttctacaat
    32701 atcgaagaaa acagaggcta tacacatatc tttaaaatga gcaccgaaaa acctgctcct
    32761 gttgctttaa catcaggccc ttacaattgc tacaatgtaa gatgggcacc cgatgcaaaa
    32821 aaaatatttt atatcagcga taaaactgaa actcctcaat tgctgtcaat gaatgccgat
    32881 ggcagtaata aagaggtttg tgacacattg ctgaacggcc tttcgagttt tgaaatttcg
    32941 cctgatggta ctaaaattct cttttgcaaa gaggtggctg ttggaaagtc ggccaaagat
    33001 ttatataccg atttgcctaa ggtaaatgtt gttattgctg aagatttgat gtataggcat
    33061 tggaaccatt ggaacgacaa cttatggagc catatttttg ttgcaggttt caataatggc
    33121 aaaattgata atgctactga tattatgcct gatgaagcct gggatgcccc actgagccct
    33181 tattttgaca atactgaaat ggtgtggagc agcgattcca gaaaaattgc ctatacatgt
    33241 aagaagctta ccggcaagga gtatgctcta agcacaaatt ccgatattta tgaatatgac
    33301 ctggccggta aaacaacgct caatgtaagc aatggtatga acggttacga taagtaccct
    33361 gtttattcgc cagatttgaa atatcttgca tggctgagca tggaaactcc aggatatgaa
    33421 tcggataagg accgaattat aattaaaaat ttggctaccg gcgaaattaa aaatatgagt
    33481 gcaaactttg atcagccagc atcgagtctg ctctgggatg agaatggaaa attcttgtat
    33541 tttattagtg taattaatgg aacacatcag atttatactc tgaatatcga agatggcagc
    33601 atccggaaag tcactgaagg tatccacgat tacacaatga tagcaaaagc agagaacgtg
    33661 ctattaggag ccaagatgag catgagtatg gcgacggaat tgttcaggat caacccggat
    33721 ggatcagaga cacagctgag ctttgtaaat aaggaaatat acgataatat tactttcgga
    33781 aaggttcagg aacgatggat tacaaccacc gacaataaaa agatgctaac atgggttatc
    33841 tatccacccg actttgattc cacaaaaacc tatccggcgt tattgtattg tcagggtggt
    33901 cctcagagtg ctgtgagtca gtttttttca ttccgctgga attttcagat catggcagca
    33961 ggaggttata ttgtagttgc tcccaacagg agaggtgttc ccggattcgg gcaggaatgg
    34021 aaccggcaga tcagtggtga ttatggcgga caaaatatga aagattatct ttcggcaatc
    34081 gatgccctcg caaaagaacc gtatatcgat gagaaccggt tgggagcagt gggtgcgagc
    34141 tatgggggtt tttcagtatt ctggttggca gggcatcatg agggcaggtt taaagctttt
    34201 attgcccatt gcggtatgtt caacctcgaa agccaatacg gagccaccga agaaatgttc
    34261 tttgccaact tcgaccttgg cggaccttat tgggataaaa acaatgctat cgcacaaaaa
    34321 tcgtatgcaa actcacccca tcgctttgtg gacaaatggg atacacccat attaattatc
    34381 agcggaggca aagactttag aataccatac accgaaagta tgcaggcatt taatgctgca
    34441 cagcttcggg gtattccgtc gaaactgctt atattccctg aagaaaccca ttttgttcta
    34501 aaaccgcaga atgccattct atggcagcgc gagttcagat cgtggcttga tacatattta
    34561 aaaaaatagt gttaaccaaa gcacaataaa aaagggtaac aataataaca ttgctaccct
    34621 ttttgttttc aggtacacat tataatttat ccattgtcat caggaattcg agattatctt
    34681 tggtgtttgg aagccgttgt ctcataaaat ccatcgcttc aagcgaattc atatctgcca
    34741 gaaactctct gagtatccac accctattga gtacttctgc cggaatgagc aaatcttcac
    34801 ggcgtgtgct tgaagctgtg atatcaacag caggataaat tctgcggttc gacagtttac
    34861 gatcaagctg cagttccata ttgccagttc ctttgaattc ttcaaagatg acatcgtcca
    34921 tttttgaacc ggtctcggtt aaagctgttg caagtatcgt cagcgaacca cccccttcga
    34981 tatttcgtgc ggctccgaag aaacgctttg gcttatgcaa tgcattgctg tcgacaccac
    35041 ccgataaaac cctgccggat gcaggagcaa cagtgttgta agcccttgcc agacgggtta
    35101 tggaatcgag tagaattatc acgtcatgac cgcattccac caggcgtttt gctttctcta
    35161 agacgatcgt gctaaccctt acatggcgtt ctgccggttc atcaaatgtt gaagcaataa
    35221 cttcagcgtt tacatttcgc gccatatcag taacttcttc cggacgctca tcaataagaa
    35281 gaatcataag gtatacttcc gggtgattgg cagcaattgc attggcgatt tcctgtagca
    35341 gtaccgtttt accggtttta ggctgcgcta caatcaaacc tctctggcct ttgcctatgg
    35401 gagagaataa atcaacaatt cttgtcgata aagtggcttt tccctgccct gtaatattga
    35461 atttttcagt tgggaataat ggagtaagat gatcgaaagg aatgcggtcg cgcattactg
    35521 aagggtcgcg gccattgatt ttattgattt taatcaacgg aaaatatttt tcaccttcac
    35581 ggggaggacg tatgcttcct tcgacagtat cgccggtttt cagcccaaaa agttttattt
    35641 gcgattgact tacatatacg tcgtcgggag agttcaaata attgtagtcg gacgaacgaa
    35701 gaaagccata cccgtccggt acgatttcga gtactccgtc gatgttaaca attccattaa
    35761 attcgaatga ttgtgtctct tttactggct cttcggtgtg atttgctatt tcgcttgttt
    35821 cagtgttttt ctgattttca tgaagtgaat tcacttcatt tttatctgca gagtcagaaa
    35881 tttctttttt tacttcgcgt tcaggcaaac ggatttcctg ccgttttgct tgaagaggtt
    35941 caacgctggc tacatgtttt acaggaggag ttagttcctt ccggtcaaca ataggctcag
    36001 cttctgatac aacgtcagct ataattgaag gttgatgtat tacctgatca ttaacaatat
    36061 gagtattagc aattatttca cttggtgcag tcacagggtt aactgcaggt ggattggaag
    36121 aaacgcttac attttcatat ttttttctaa aacgcttctt acgtttttta ttttcaggag
    36181 cttctgggtt ttcttttttc tccttcttct ccggttcgat cgattgattt atcttagtaa
    36241 ttaaagcact ttcgtcttgg gttttttctt ccggaagcac aggcctgcgt ttacgatgag
    36301 gaataatcac atctttgtta tcgggagtgc cggagttttc tgactttgca tcttctgacg
    36361 agatttttgt tgcttcctcg ggtttgcttg tgggaaacaa tgtgaagtcg cggtacgaag
    36421 gggtatttat cgt
//
LOCUS       DAOOQP010000014        36300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1351_length_36300_cov_5.644806, whole genome shotgun sequence.
ACCESSION   DAOOQP010000014 DAOOQP010000000
VERSION     DAOOQP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36300
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_36300_cov_5.644806"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLK75_02750"
     CDS             <1..1215
                     /locus_tag="PLK75_02750"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38193.1"
                     /translation="CEFNTSQPAAQRNRVHIYNHDGSNFSEDWPKNLPHRPAVTPSVA
                     DVNLDGNPDILVCSTDSLYLFDLQGNNIDPFPWSLPGMKFSYQSPIIVDYRSAPFEDY
                     YPVEIIGANHGDNPGYYKIIYTSAEVSMLHWNTDDMMWTYSTPMAFGGQNTMWYELVS
                     QPLGDYENGDSCIFNMCNSVQQAEFPEINVVRYDGLEGFMSYNGYSNFLFTGSNCFDE
                     NENGFLHIYNCNGPVFTELNGLPVKVRGKTFMNGINLGDVNGDGMLDMVIMGCSDSTY
                     INVIEMTSIENLGFFPYFDPWYTVGCYKGDNTRQGRTEPVTYEKLTNELEISVKLFPN
                     PAHEEFLITWDDAMESLYLKIFDLVGRQLEYRIIQSGTLLNISQYSKGMYIIEMKNSD
                     GKVVFSEKLLVE"
     gene            complement(1223..1915)
                     /locus_tag="PLK75_02755"
     CDS             complement(1223..1915)
                     /locus_tag="PLK75_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38194.1"
                     /translation="MKSKIFFFFVIVISAGMFFTSCETEDSTDVNQDRIYAYYELFYS
                     ADQDVTFARASFRFGGITGTLLELTSPAYVKFNDNYMTYKPALAYYEASFPGLVAEGD
                     FTYYDLDSNIFVNHAVMHAIDLEETLDTIVRGHVYELSFTGDSLRWNEIATAFINSSL
                     GTDLQTFLQSELLSKSIIMPSERTLSMTQGVNTVYVKRNYTNQVEEATTVGAEIHSIW
                     QSHSRQVYVDVE"
     gene            complement(1920..2771)
                     /gene="prmC"
                     /locus_tag="PLK75_02760"
     CDS             complement(1920..2771)
                     /gene="prmC"
                     /locus_tag="PLK75_02760"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOY38195.1"
                     /translation="MTRKQIHKDLTHKLAGLYPVQEADAIACICLQYAGGFDTLQYHL
                     NIDKAPDHNELSTIEACTRRLLAYEPIQYITGHTWFYGFKFHVNPAVLIPRQETELLV
                     DIIIKENLTKQPQQILDIGTGSGCIAISLAVQLSESNVTAIDISLEAIAVAKMNAELN
                     KAHVEFNVSDILDENCIISQPYDIIVSNPPYVLRSEVVHMLNNVTEYEPHSALFVDDN
                     NALCYYESILQKSRSCLKQKGCIYFEFNETKTLEMKALLSKYQFDNMQFYEDFQKKCR
                     FVRVIKN"
     gene            complement(2844..3863)
                     /locus_tag="PLK75_02765"
     CDS             complement(2844..3863)
                     /locus_tag="PLK75_02765"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOY38196.1"
                     /translation="MVELNWSQLPFGYVKTNINVRAYFKNGAWSKPEITDSEFLNIHM
                     AATALHYGQEAFEGMKAYMGKDGKIRLFRWEENWRRLQNSADGILMEPVPKDLFFEMV
                     KTAVLRNKEFVPPYGTGATLYIRPLLFGSGVRVGVKPADEYMFLIFVTPVGPYFKEGF
                     NPIKIAIVRDSDRAAPLGTGTFKVGGNYAASLKGGERVHKAGYGSPMYLDAKEKKYID
                     EIGAANFFGIKNNTYITPSSTSILPSITNKSLIQLAEHLGYKTERRPVPEEELNSFEE
                     VGACGTAAVISPIGEINDLDSGHVYKFCKDGKPGPISTKLYETLVGIQFGDIPDPFGW
                     ITYLD"
     gene            complement(3977..4894)
                     /gene="queG"
                     /locus_tag="PLK75_02770"
     CDS             complement(3977..4894)
                     /gene="queG"
                     /locus_tag="PLK75_02770"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOY38197.1"
                     /translation="MAADHLTNDTIKKAAHMLGFDGCGISSAFVPDSIKVKYEKAISL
                     PGYQALEYIRKNLRLRFAPDLLVENALSVISLFAYYSFIPDSKKNVHIARYAHSSDYH
                     LVLKNQAYKLLKKLQEYDNSVEGRVFVDSGPVMEKHFAVLSGIGFQGKNTCIIHPRLG
                     SFGFLCEILINKKVEYDTPVPYSCGDCSLCVDACPTSALRDYQLNPEKCISFHTIENK
                     QSVPEEIRARAPQLWFGCDICQEVCPYNKIQMDSRFFIPENAVQDLSYDELVNMNEEN
                     FKECFSKTPLGRAGLKKLRNSAGIKDKDI"
     gene            complement(4903..5958)
                     /locus_tag="PLK75_02775"
     CDS             complement(4903..5958)
                     /locus_tag="PLK75_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38198.1"
                     /translation="MSASILQAQHISVYSDYLDKVYVFDSGNHRQIEHLPLKSYKIGN
                     NAMAYEDNTGNFKVFYNNYLFSVSAFATEYQVTDNLVVLRMNTQLKVFDNGQIKNLSS
                     NISGFAAGDDVIVFFDDLKHKLIAYYNSQFFELDDALNLDSLSNFKCGENTIVFCDSK
                     NNFYLFYDEKIIPIANCERIKSVEAGRDIVAFVEEPINSFQIYYNDEIIELESFEPLS
                     YHCGDNLLAYVDADNYLKVFANGELFTISFDTPNFYEVTDELVVFSVQNDFKVFYREK
                     SYTLENVVPERFIANNDMVAYIDVHGYLKVFQNGVTKTISYEKIDDFEISGNCVRYSF
                     GVQSQNIYWNGNTYSNN"
     gene            6121..7161
                     /gene="thiL"
                     /locus_tag="PLK75_02780"
     CDS             6121..7161
                     /gene="thiL"
                     /locus_tag="PLK75_02780"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOY38199.1"
                     /translation="MKQNQSVESIESLGEFGLIEYLTKDFITHCNSTVKGVGDDAAVI
                     DCGDYFRLITTDLLVEGIHFDLIYTPLRHLGYKAVTVNLSDIFAMNGKPEQITVSIAL
                     SNKISLKAIEEIYEGIRLACNAYEVDLVGGDTTTSLTGLLISITAIGTARKQEVCLRS
                     GAQVNDVICVSGNLGASYLGLQLLEREKQIFIADPHIQPEIGKYPYCIQRQLKPEARK
                     DVVENLKKADINPHAMIDVSDGLSSDLLHICKQSKVSCKVFEDKLPIDTETASLACEL
                     NIDPTIAAMNGGEDYELLFTIDSKELEKIAKIDGITAIGYVTSANNRPLLIARNGVEI
                     ELKAQGWNHFDK"
     gene            complement(7176..9380)
                     /locus_tag="PLK75_02785"
     CDS             complement(7176..9380)
                     /locus_tag="PLK75_02785"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY38200.1"
                     /translation="MIKRIPYFLLLISVFLLSREKAAFTQELKEYYISCNPDDFRMIY
                     ENYNEEIYIPISITHNNVTLTKARMRIRGDGTVVLPKKSLKIQIDSLCFDDGIRVFNF
                     NAEYEDKSYLQQYICSRMMQESGLWCFRTEHVRLYLNGEYLGLYLYVENMDEDFLTRN
                     GFNPSGNLYKATVDGACLSRYDNVFYHWEKKSGNSDYSDLQKLINQLDTVSISDYYSF
                     AFTEFNYQNLINFLAVNLLIRNNSTYYHNYYMFNDSYSTRKWHIFPWDLDKSFLYYEV
                     NIPYYHTSRYWTPDNPFAELAFLNDSTLQDIRERITELHSTLINNEHVEPWVDSLQLL
                     LYNSVEHDTTDNINTISDWLSQINTVKTAFDSRYYELMVQFEQIPRSFRVDRVNNRYF
                     TGEPIVFRWTKCEDPLGNEIKYRLYYGKNINLVDGTANMIENISDTLLILSDTIEPGK
                     YYYKVVAYTQNYHLDGFDNYNIFFVDNDTSQIIINEINYHPDSDVDPGEWVELYNASA
                     YDVNVSNWYFCDNNDLHRYTFPTGMVIPSGSYYILANEPQKFLQLFPASECNIDVFNF
                     ALDNSGELLRLYNSYGRLVDLLVYDDSIPWPEEADGDGRVLALNSPLLDNSLGRNWNA
                     VKYYGTPCKENQSSDTTINPIPVNTMLRCFPNPCSETATIVYHSSAQSNTSITITDIT
                     GSIVFCKEDFYENEGYYRYVWDANVVNDGLYIVYLRENSQIISSFKMVKTTF"
     gene            complement(9373..11124)
                     /locus_tag="PLK75_02790"
     CDS             complement(9373..11124)
                     /locus_tag="PLK75_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38201.1"
                     /translation="MKKCISIVFFIFWANVIISQTPDSAVIICPSQSTSNDIFPVVVR
                     LYKNGALNYAGHSYSLAVTNATAESSMIVVKKGVGSLCTKINTSADFQVEVQGFSTIK
                     NVNHTINDPIEISGYINSDYQCIANTLYYVSSDLTIQASQTLTIYEGAQLIVAENANI
                     ICHGKIICDANTENPIVFKSDSENIFWGGITITSAQDTSVFEHVFFIKGGGNTNFIFG
                     HSGSQPVLKADNGNMICRDCYFIDNTGKALGGNYANISLKQCLISRCDTGGEFFMSKV
                     DIDSCYVMNIPDDDGIIEDDDNDCFYFNNVHSSNSESKVSNSFFITGEDDAIDHNGAK
                     LSLENCWIQDFYHEGIAGSNGNYVSLHNSVVVDCGQGIEAGYGSPHVMANHCVFAHNG
                     VGARFGDDYVQPSEGKMIIKNSIFFDNTDNLLNWEPQTGGPVTDSLEVMYSITNDIDF
                     DGQNSCIAGTPQFSQAYLLEIGSPGSGAADDGLDMGLISSLTMIGQIMQHTSVQLYPN
                     PVNTVAVFDFDAPYPEFGDLSILNMEGKILLNRTIHFDTGINKIEQNVSFLKPGLYQL
                     VVVTPTKVFSSKFLKLD"
     gene            complement(11235..12023)
                     /gene="lpxA"
                     /locus_tag="PLK75_02795"
     CDS             complement(11235..12023)
                     /gene="lpxA"
                     /locus_tag="PLK75_02795"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOY38202.1"
                     /translation="MNQPLAYVHPEARIAQNVVIEPFVTIAKDVVIGEGTWIGSNVTI
                     MEGARIGKNCKIFPGAVISAIPQDLKFRGEITTVEIGDNTSIRECVTVNRGTIAKGKT
                     VIGSNCLLMCYSHVAHDCIIKDNVILGAYVALAGEVIIDEWAIVSAGSLVHQFVNIGA
                     HTMIQGASKVGKDVPPYVKAGRDPLSYEGVNSIGLRRRNFDNEKINELQNIYRIIFGR
                     GMNTTQSVDFIEAEMTASKERDEILLFIRNSKRGIIKGYFGNIE"
     gene            complement(12028..13443)
                     /locus_tag="PLK75_02800"
     CDS             complement(12028..13443)
                     /locus_tag="PLK75_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOY38203.1"
                     /translation="MAEQNRKQKTVNKTVVLQGQGLHSGVETKLVIKPAQPDTGYVFI
                     RTDISPEASVKAIADNVTDTSRGTTISNNGTSVSTVEHLLAAAYSLGIDNAIFELNGP
                     EVPILDGSSHYFVEAFYKAGIKEQNAFQKVFNLTETLSFSDKERGVDISAYPDSSYKI
                     KVLVDYNSEVLANQYANLDSLEDFTNQISKCRTFVFISDLEPLLRLNLIKGGDLDNAI
                     VIVDREWTQEEYDRLTKLFNKPKVKVQSLGILNNLVLHFPNEPARHKLLDVVGDLALT
                     GFRFNATIVASRPGHYGNTQMAKIIRNHILQQSTINEKESRIPEYNADVKPVMDVNKI
                     KTYLPHRSPFLFVDKIISISDTEVVGIKNITNGEYFFQGHFPGEPVMPGVLQIEAMAQ
                     TGGILVLHSMPDPENYTTLFLKINNVKFRKKVVPGDILIMKLTLLEPMRRGIAHMRGE
                     SFVGKDLVAEAEMHALIEKQK"
     gene            complement(13632..14663)
                     /gene="lpxD"
                     /locus_tag="PLK75_02805"
     CDS             complement(13632..14663)
                     /gene="lpxD"
                     /locus_tag="PLK75_02805"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOY38204.1"
                     /translation="MEFSAKQLAQILGGQVVGNPDIKVSNISKIEEGEAGTLSFLANP
                     KYTPFIYSTKASIVLVNNSFEPEKPISATLIKVENSYLALAKLLELYNTETSQPEEIH
                     PMAFIHSEATIGKNVYIGAFACIEKGVVIGDGAQIYPHVYVGKNSKIGQKTCLFSGVK
                     IYHDIVVGQHCTIHSGTVVGADGFGFVPQSDSEFIKVKQVGNVIIEDNVEIGANTCID
                     RATMGSTCIHKGVKLDNLIQVGHNAEIGDSTVVAALSGVSGTVKIGKNCMIGGQSGFA
                     GHIKVGNGVMVGAKTGVSHDVEDYKKMLGAPAMEGHEYHRCFAVFRHLPDMLARLNQL
                     EKHIKDKEK"
     gene            complement(14686..15915)
                     /locus_tag="PLK75_02810"
     CDS             complement(14686..15915)
                     /locus_tag="PLK75_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY38205.1"
                     /translation="MNKRKIVNDPVYGFINIPNSIVYDLIEHPYFQRLRRIKQLGLTN
                     LVYPGAFHTRFQHALGAMHLTTLAIEVLRQKGHVISDEEAEAALIAILLHDIGHGPYS
                     HALEYCIIPGISHEELSLAIMNELNTVFNGRLSLAIDLFQGKYHKKFLHQLVSGQLDM
                     DRLDYLRRDSFFTGVSEGVVSSDRIIKMLEVVDDELVVEAKGIYSIEKFLIARRLMYW
                     QVYLHKTVLASELMLTKCIGRARQLVQSGIHADCSEPLSYFLILKHFEISPEMLAHFT
                     KTDDYDVMYALKKWTDHDDFLLQYLSSGIIDRHLFKVKLEKEPFSSDYIEKLRKQAAK
                     HFNIGINEASHLVFTDAVSNNAYSFADEKINILVQGRLVDVTEASDMLNHSVMSKTVR
                     KHILCFPTEIQHVINQP"
     gene            16088..18694
                     /locus_tag="PLK75_02815"
     CDS             16088..18694
                     /locus_tag="PLK75_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38206.1"
                     /translation="MRNLLLLALITLLWSTSAFSQNDEDFDKFKREYYNGLEDLRNDY
                     KRFVEQNDKEFAEYIQKEWQQFQLFKAEYASPAPGPDVVPVYKKLINVEPVRRIQPSQ
                     IQLQTKTIEMAVEPMNPIPAITSADKDLRDNNICFDFYGTHLCIPVPDDMRSTFSASV
                     DEQNFASYWKKMSDSGFDAPIVELLRVKSNLNLNDYAMYLLVNELGTRATKDRNSATL
                     FSWFVLTKMGYKIRLAYHSKGVSLLVPVFNQLYGFSYLTFGGVKYYMLDGTTGAVKTY
                     EKDYPAANQIMNFNLYKPPSFDVDTLTRNIGFSFDSKQYNIKVLYNKNLIDFYSSYPQ
                     CYIGLYFDAGVSEITKESLATELWPIMKGMPEKDAASFLLKMVQTGFSYKTDQEQFGK
                     EKYFFPEEILHYEACDCEDRSVFFAYIINELLDLPVISLNYPQHVSTAVCFTSMVNGD
                     LIQYDNRRFTVCDPTYINAPVGATMPQFKKSKFSIISPSKRSNQDEQEEFVWEKIYKA
                     GGFKGSDKTILISEDSSCLIAGFFTDTLTMNGRSWVVENENTGVFVARLDKYNKPLWV
                     STFATDGSISLQNIIISSDRSVYVSGEFGKNLYFDKKKLVSSSVADQFAIKFNKDGEL
                     EWQTSIRLDSIYGGKPFFLQVTISDRGDVKGLTFVEEFSYNPEQTLFLNTENDLVIIA
                     ERGVSESGLKISPELGLSGNYELLETWKKLTDKYISMHYDKSIAGFFALMETLQNSSL
                     DISGKEMMASLNTLNPSFKNNYKNFYSSLQYLTRISSRNGIVTFYTSGGKVLKCGPLS
                     IVNGARAQMRDYKSGNKQVKVLNGIYYDSFFRSLRVNYLKCFKVNGDIMIDYDNEHYQ
                     KVVNTQTDILKQ"
     gene            18752..20116
                     /locus_tag="PLK75_02820"
     CDS             18752..20116
                     /locus_tag="PLK75_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38207.1"
                     /translation="MRNAYPIAAAFLIILISVSCASSRLTKKGSKMEEAGMYVDAAEY
                     YYQAVLAKKSNVDAKLGLKRTGQRVLDKKLSAFNKSYNEEKNQEAVYNFKDAEDYYNK
                     ITAVGVELNFPSYYRENYDEVKNVYIADKYNEASKLLENEKFAEAEKLFREILQLQPG
                     YKDSKEKLNVAIYEPVYRDAQQKMNNKMYRAAYYLFDKVISGSGNYKDAYELKAECLE
                     KATLMLAVSPVVNKSGTAGIETSLQAAIIKDIQDKRNPFLKLVEANPRLQPDGSLSKG
                     QQPPVKAVNPDITLYTTVTGFTYEKGMLKETEKRGYRKYTKQTKDEAGNVRTVTDYEK
                     VTYKEYSMGRQVKISFTYKLIYNKTGEIIATNSHTLVSEDKILYADYTGDKKALVPGY
                     WKNKAGHSSEDVVNDNQKDVKALQELLDGRRVIKDYNTLTGEVISASSMKVADAVNEY
                     NPEE"
     gene            20119..21495
                     /locus_tag="PLK75_02825"
     CDS             20119..21495
                     /locus_tag="PLK75_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOY38208.1"
                     /translation="MKQTVILFLLAGLLMSGCKTLQNTTEAVKPQPEWVKSKPVSSFY
                     YIGIGSATKPGFAPADYTQTAQQKALGDLAREISVTISSASVLSVMEHNYNITENWTS
                     EVTTSTETYLEGYELVDSWEDKNYYWVYYRLSKEKYAQMQAERKNKAIADAVYKYYEG
                     QTQRKANCYTEALRFYADALTALKPYMNESTETIVDSVKADIGQTVLNAMVSMIQSIV
                     IEHPYTEIKVIRGTSLSQTERSFIVYNQNRQVIADLPVEIKYTGVELKNERAVSDNSG
                     KVMCDFNKIVSSGETETISLSVDLITMSRVSKDPFIRKIIKQLPVKRTSVVLIIEKPA
                     FFVISTEKEFGTETGKALLSDALRPEISRANKIADTQSQADFVVQIDANTVVREKVGY
                     ETITDINVMVTVFDKKNEQKFQKQYVAEGEGQNSKESSNDAYQEAVRLITRKAINDIN
                     SALFSNLP"
     gene            21506..21820
                     /locus_tag="PLK75_02830"
     CDS             21506..21820
                     /locus_tag="PLK75_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOY38209.1"
                     /translation="MSVLMSLVIFPTDKGSSVSKYVSRVVEIIRDTGYDYRLTPMATI
                     IETPSLNHCTEIIEKAYSAIGADSERVYITATFDIRTNGTEQRLNSKIASVESKIGDV
                     KK"
     gene            21943..22650
                     /locus_tag="PLK75_02835"
     CDS             21943..22650
                     /locus_tag="PLK75_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38210.1"
                     /translation="MVSVNDLFGCLTKVENLWCLGEYGVRNTCILESLDTFPGYYGNA
                     PTTSKPLYVYIVAGEHYTMEDIVRISLKVKADHKSPFDAAFTEISMRHATCCAVRIAG
                     VTNYDNVKELQECFQHHGLVLKKKVNNIENSPALIKIYKFFLLDPVGDNIFLDLRNPD
                     IGYFDVYENPAWDEFSSRIQVLRNNISGQSFDAAKSFVFKGAEIQDMVRIYCKNLTLE
                     FLKGLKEKYYLLFANGR"
     gene            complement(22658..23884)
                     /gene="hydF"
                     /locus_tag="PLK75_02840"
     CDS             complement(22658..23884)
                     /gene="hydF"
                     /locus_tag="PLK75_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOY38211.1"
                     /translation="MSRGKENKPHIGIYGNRNAGKSSLINRIAGQEVSIVSDFAGTTT
                     DPVKKSIEITGFGPVIIIDTGGIDDIGEIGLKRVSKTLHSIASIDLAILVITNNIISE
                     SEKKLIYSFKQHDVPFIIVHNKSDLRPLDPTFKTSLKNDYSTDIIEFSTLTDNNLQPL
                     IDAIKNNIPDLSWKIQSLLGDLISYGDIVLLITPIDIEAPEGRMILPQVQAIRDILDN
                     DCIAIVVKEREVDVFLRKTGIKPALAITDSQVFLKADASIPKEIPLTGFSVLLARYKG
                     DFTNYLAGTPKISELRTGDRVLILESCTHHVSCDDIGRVKIPRWMSNYTGKKLDFDVV
                     TGLDKLPRDIRDYALVVQCGGCVLTRKQVINRLRPAVEAGVPVTNYGMAIAWMQGIYK
                     RAVAPFVSLDNKSEYL"
     gene            24013..27687
                     /locus_tag="PLK75_02845"
     CDS             24013..27687
                     /locus_tag="PLK75_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOY38212.1"
                     /translation="MYRLLKTIFTSALVIICFSSAFSQLVEHGLPGFYNYAPKEYKNE
                     SQNFSITQDKFGFLYFGNLNRIIRFNNVEWQTVHLIGTPILDVSENGLIYAGGYNKIG
                     VITPYINRMEMKDLALPPSCSPGQINKVIAFNNHVLFLGDNMILDIKDEVVEKVYSSE
                     NKILAFKANKKIYVSIEGNGLFTYDPDKRTMQPVAKLILPENRDVIDLFVLDERNILI
                     KCENTSGFYLFDGTTTLPFTTDADAFMEENQYIKGITLSDGSIVVGTEYGGIICVSST
                     GQYLFSMNKNHGMLDNRITDLFVSKNNKLWVTSYNGITSVNYPSPVDYFGNSYGINGA
                     IKTMIRYNGTMYFGSTQGLYYYTTTNLAKSKKLINNEDYFAKVEGVVAEIQDLIIIDN
                     QLYIITSKGVYKLIREGRARLSIPGEFRKLKVSEVYDDIVFCIGEQGILVLRKELDSL
                     HIVGKVKNFDYEVRTMVHDEKDVFWLGSDNHGLLKLDFRKGDLLNPQIQRFDENNGLP
                     ENFTWVDVYRTKAGILFSTQCGVYYNNAGKFIRHPLFETPDSSNVFSFYPIVEQADGK
                     LWYSSTNTKNSERKTGYITYDKDFNPTDHSNELNNLKDRNIESIYCDSNNVVWFGGFD
                     GLIRYEANKMETSTPQALCLIYNTDLPDSINNKLFDRSGKQILTPVLKSSQNHIRFEF
                     TALYYNSYKEISYRTQLVGLQDEWSKWSGESFKEYTYIPPGTYTFKVQARDIFGNTTE
                     SHGFTFSVPIPLYMRWWAFVIYFLFLISFIYLILKLNELKHATDKNKLEQTVAERTDE
                     LIRQKEQTQKLVQKLLPDRTAEEIQKEGKAQTQAYERVTVLFADIEGFTKIAATTDSE
                     VLFTQLNKIFSSFDNIIEKFEIDKIKTIGDAYMCAGGMRNNDRTTPIEVVMAALKMQE
                     AIQEINETDKCNFRIRIGIHTGSVIAGVVGAQKIEYDIWGDTVNVASRMESNAISGTV
                     NISSSTFDYIKDFFECESRGKTAVKYKGDLDMFTVVKYKQHLSEFSTGKTPNKLFYTK
                     LQALRYVDLENYVMKKLETDLPKNLYYHNSRHTTNVCTRVENIGREEKVTDEELLLLK
                     TAALFHDAGFMISYDNNEPLGANLAEDILPKYRYTPEQIQKIKALILSTKMPPKPKNH
                     LEEIICDADLDYLGRTDFIVVSQNLFRELYERGKVKTIEDWNRFQYKFIIKHEYFTAT
                     ARKLRESGKKAVIEELKKRI"
     gene            complement(27684..27908)
                     /locus_tag="PLK75_02850"
     CDS             complement(27684..27908)
                     /locus_tag="PLK75_02850"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOY38213.1"
                     /translation="MIKDKLITIAKFRYEYRAYLLKDRLLEAGIECALTSESVFKLID
                     GVMVLVKESDFARAMEVYNALKESSDDFED"
     gene            28024..29580
                     /gene="nadB"
                     /locus_tag="PLK75_02855"
     CDS             28024..29580
                     /gene="nadB"
                     /locus_tag="PLK75_02855"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOY38214.1"
                     /translation="MQKIVDFLVIGSGIAGLSYALKVSRKGSVCLVTKNALEDGSTLY
                     AQGGIASVTYQPDSYEKHINDTLIAGCYLNREDVVKMVVNEGPSRIEELIQWGAHFDK
                     NRDGRFDLAREGGHTEYRVLHSKDSTGREIQQALIHQVKLNSNIEVLDNHFAIDLITQ
                     HHLGFLVKRIYNDIECYGAYVQDKHTGKIVTILSKTTMLATGGAGNVYQNTTNPSVSS
                     GDGIAMVYRAKGIIDDMEFFQFHPTALYHPVDRPSFLITEALRGHGAILKNQKGIPFM
                     KKYDSRESLAPRDIVARAIDSEMKISGHDFVYLDATQIDANDLQNKFPNIYQKCKSYG
                     IDITREYIPVTPAAHYLCGGIVTDIYGKSSINFLYAAGETACTGLHGANRLASNSLLE
                     AVVFAHRAAEQAMSDIEKACIKTAIPDWNDEGTIHNEEMVLITQSLKEMRQIMSNYVG
                     IVRSDLRLKRAFERLEILFQETESLYKSSKITPEICELRNMINVSYLIIKMAMARRKS
                     IGLHYNIDHQ"
     gene            29663..31822
                     /locus_tag="PLK75_02860"
     CDS             29663..31822
                     /locus_tag="PLK75_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOY38215.1"
                     /translation="MKKIILILVTIVLYANSFADGIKLNDAQYAAKTYYYQNVQSFRA
                     QGWDEINLQLVNNPEEGVYPMYIFNVNENEGFIIVSALNTMKPVLAYSFEGAFNPGNM
                     SPGQTAFIQYFADETMLALSGDIVVKEGVSAEWDRLLRYTPGTSFEKDVTVGPLVNAK
                     WNQNWPYNASCPVDDDCPDYSSDNHDHVYVGCVAIGMLQVMKYYNWPPSGEGSKTHLS
                     WTNGGYGNITINFAQQTYNWYAMPNSLGGTENSEVAKICYHAGVAVSMWWGCDGSGSQ
                     TDKIVTALENYFKYSSDAQFIEKSDYTDSNWKTVLRNQLNQNKPMVYGGTSTTVGHAW
                     TCDGYQNTDYFHMNWGWGGAGDGFYALDNLVSTATPGGPENDFSTGQEAVINIYPESG
                     YPLYCGQNINLNTPSGAFGDGSSNLDYQNNQQCSYLIQPTCGAVVQLNFDSFDLAEGD
                     IVRVFNGSNADAEVIETYTSENTPGTDNIYADRGAMYIQFITDGSGVASGWDASYVVK
                     NCKTNIVYYEPSGSFDDGSGVCDYGSSSSTVCTWFIQPEDAAWINIHFTDFHLAGSPD
                     YVKVFKNSTGSENLVAQFSSTALPTTDIMVEAGVAVVQFFANTTGNDEGWALEYTSST
                     TAVENLSLKPVVQVFPNPSEGNATINFYSQGSHEATFVISDITGKLIANKRIASSDCI
                     FHTSVQDLVGNSLPDGLYILEIYSGSNSVTEKVMITR"
     gene            complement(31819..32655)
                     /locus_tag="PLK75_02865"
     CDS             complement(31819..32655)
                     /locus_tag="PLK75_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38216.1"
                     /translation="MKIKTITAFTDIKTDNIRYKSSYKVFDENGFLVEEYRFDKNGEI
                     ISKSENIFDNEHRLLSSTFFDTEQESSENRTYDYSSEGQLIRETVDFGQGYLSVFLYE
                     YSNSGKRITISEQDEEGEVEETKIQELDDYGNVIKEVVINEDGKEITHIFSSFDNKKR
                     PVRKEERDLNYKTTQIQEYIYNDNDFLEMIKTTNAKNQILNWVKLEYDELNRPVKQTS
                     MSGSVILVEYIDDKTRIEKHINSHGYTYSESKITVNEWGNLLKEDSPEIVTEFEYEYF
                     ES"
     gene            32736..33671
                     /locus_tag="PLK75_02870"
     CDS             32736..33671
                     /locus_tag="PLK75_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOY38217.1"
                     /translation="MEKLLFIINPKAGNGDLEKVWGEVEQTIRRHDIEYHHYFTTAQN
                     DGCEQVKLFLKQGYRNIVVLGGDGTINEVVNGIFQQQDVPYEEVVMGLISMGTGNDWG
                     RYYKWTTNHIDAIHQILDGNFIRQDIGVIKHWINGQQHTSYFANIAGFGFDAAVVKAT
                     NIMQARGRRKKSAYLLNLLKCLINNKLLEMKIEFDDQIIHDSIFSISIGNGKYSGGGM
                     MQTPRAINNDGLLDIAIYRNIRKLKVISNVNRLYNGTIEKVRGLETYRSKSFKISCPH
                     TTFAEIDGEIVGDGPYEISILPNALKVFAKPSLKI"
     gene            33822..34337
                     /locus_tag="PLK75_02875"
     CDS             33822..34337
                     /locus_tag="PLK75_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY38218.1"
                     /translation="MRNEKENILLEGCRNLEPRAQKRLYDAYKNCMFTICMRITGNYE
                     EARDALQDGFISVFNDISKFRGESSLGAWIKTIIIRAALKRLKPVSFIASDEIPDAPV
                     EWDSEFTGEELQKAIWALPEGYRVVFIMFEVEGYSHTEIADILHISEGTSKSQLHYAK
                     KKLKELLKSYR"
     gene            34339..35517
                     /locus_tag="PLK75_02880"
     CDS             34339..35517
                     /locus_tag="PLK75_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38219.1"
                     /translation="METDNLKKAISQLPQYKAPDVWGKIKSEIPRKSPGKLLFFFTAA
                     IIGCSVVAGLLLSTPETILSSECFQARTPNRPLMRYVKDVEEPETAEVILKFNKSNKK
                     TEISTPDTTAKLQTIPQTSNEIAYETPPEPPSFYHIITSEKIKYEETGSNLITNGDFE
                     EYVFCPAGFTERPVRKLIPYWEVPSKGTPDYFNVCSKRDAGVPKNFAGRIFAHSGSGY
                     AGLILRSSFTNENKITGVKPLDYREYIQAELNETLVRGKKYKIQFWVCNSSGSRYAVD
                     AVGAVLTRDKIKAENDGVLDYTPVIKNSRNNMLVNQSYWVAIEGVYEAKGGEKFLTIG
                     NFNYNFATLYIMMDPSSKFNYAYYYIDDVVVVEVIETLETEYIILDSTVNVAQTCSPQ
                     "
     gene            complement(35526..>36300)
                     /locus_tag="PLK75_02885"
     CDS             complement(35526..>36300)
                     /locus_tag="PLK75_02885"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOY38220.1"
                     /translation="IQTSLWNTLVDQGLSWELAIKLSQTFAWTIDFYGLQKGDYFKVL
                     YSELAVEGKSIGNIEIESAVFRHGNKDLWAIPFVQDSILQFFDTSGMSMKKSFLKAPL
                     QYSSVSSRYTNARMHPIFHKVTEHRAVDYSAPAGTPVFAASDGRIAIRAYESGAGNYV
                     KVIHNSTYSTVYMHFSSFGQFQVGDYVKQGDIIGYVGSTGWSTGPHLHYEIHENGYKI
                     DPLSFEAPPAEPVKPENIAKFNTEKRKWITALNKINNPL"
BASE COUNT        11191 a         6920 c         7259 g        10930 t
ORIGIN      
        1 tgtgagttta atacttctca acctgcggct cagcgaaaca gggttcacat atataatcac
       61 gatggcagta atttttcgga agactggcca aagaatctgc ctcaccgccc tgccgttact
      121 ccaagtgttg ccgatgtgaa tttggatggc aaccccgata tccttgtatg cagcaccgat
      181 tctttgtacc ttttcgattt gcagggcaac aatattgacc ctttcccatg gtcactgccc
      241 ggaatgaaat tcagctatca gtcacctatt attgttgatt accgttctgc gccttttgaa
      301 gattattatc ctgtcgaaat tatcggagcc aaccatggcg acaaccccgg atactataaa
      361 attatttata cttctgccga agtatcgatg ctgcattgga ataccgacga tatgatgtgg
      421 acttattcga ctccgatggc ttttggggga caaaatacaa tgtggtatga attggtaagc
      481 cagcctttgg gagattacga aaatggcgat tcatgtattt ttaatatgtg caattcggtt
      541 cagcaagccg aattcccgga aattaatgta gttcgatacg acggattgga gggtttcatg
      601 agttataacg gttatagtaa tttccttttt accggatcaa actgttttga cgaaaatgag
      661 aatggatttc ttcacatata taattgtaat ggtccggtat ttacagagtt aaatggatta
      721 ccggtaaaag ttaggggaaa gacttttatg aacggtatta atctgggtga tgtaaatgga
      781 gatggtatgc ttgatatggt tataatgggt tgctccgact ctacatatat caatgttatt
      841 gaaatgacct caattgaaaa tttgggattt tttccatatt ttgacccttg gtataccgtt
      901 ggttgttaca aaggcgataa cacaagacag ggtcgcacag agcctgtcac ttacgaaaag
      961 cttacgaacg agcttgaaat ctctgttaag cttttcccga atcctgctca cgaggagttt
     1021 ttaataacct gggatgatgc tatggaaagc ttatatctga agattttcga tcttgttgga
     1081 aggcaacttg aatacagaat tattcagtca ggtaccctgc tgaatatctc acaatattcc
     1141 aaaggtatgt atataattga gatgaaaaat tctgatggta aagtcgtttt ttcagaaaag
     1201 ttgctggtgg aatagatcct tactattcaa catcaacata cacctgacga gaatgcgatt
     1261 gccatatgga atggatttcg gctcctacag tggttgcttc ttcaacttga ttggtgtagt
     1321 tacgctttac atatacagta tttaccccct gagtcattga gagcgttctt tcggagggca
     1381 ttataatgct tttcgacaac aattcagatt gtaggaaagt ttgcaaatca gtgccaagcg
     1441 aactgttaat aaatgccgtt gctatctcat tccagcgcaa cgagtctcca gtaaaactaa
     1501 gctcatatac atgacctcta acaatagtat cgagcgtttc ttcaaggtcg atggcatgca
     1561 ttaccgcatg gtttacaaag atgttgctat caaggtcata atatgtaaaa tcaccctcgg
     1621 caacaagccc gggaaaggaa gcctcataat aggccaacgc tggtttataa gtcatgtaat
     1681 tgtcgttgaa ttttacatat gccggactgg taagctcaag tagtgttccg gttattccgc
     1741 cgaaacggaa cgatgcccgt gcaaaggtaa catcctgatc agcactatag aatagctcat
     1801 agtacgcata aatgcgatcc tgatttacat cggttgagtc ttctgtttcg catgatgtga
     1861 aaaacatgcc agcgcttatt actataacaa aaaagaaaaa gattttcgat ttcataatat
     1921 tagtttttaa ttacgcgcac aaatctacac tttttttgga aatcttcata aaattgcata
     1981 ttatcaaatt gatattttga tagtaatgct ttcatctcaa gtgttttagt ctcgttaaat
     2041 tcaaaataga tacagccttt ttgcttaagg caactccttg atttttgaag tatgctttcg
     2101 taataacaaa gcgcattatt gtcatcgaca aataacgctg agtgaggctc atactccgtt
     2161 acattattaa gcatatgaac tacttccgat cgaagtacat agggaggatt gcttacaatt
     2221 atatcatagg gttgcgaaat aatgcaattt tcatcgagaa tatctgaaac attaaactca
     2281 acatgggcct tatttaactc agcattcatt tttgcaactg caatagcttc aagcgaaata
     2341 tcgattgctg taacattgct ttcacttagt tggactgcaa ggctaatagc aatacaacca
     2401 gaacccgtcc caatgtccaa aatctgctgc ggttgtttcg tcagattttc ttttataata
     2461 atgtcaacca gtaattccgt ttcttgtcgt ggaatcagca cagccggatt aacatgaaac
     2521 ttgaacccat aaaaccaggt gtgcccggtg atgtattgta ttggttcgta ggctagtagt
     2581 cgtcgggtgc aggcctcaat cgtactgagc tcattgtgat caggggcttt atcaatattc
     2641 agatggtatt gcaaggtatc aaatccacct gcatattgaa ggcaaataca ggcaatagca
     2701 tctgcttctt gcacagggta cagtcctgcg agcttatggg tcaggtcttt gtgaatttgt
     2761 tttctggtca tgctcactgg gcatcgagat atacaaaact taaaatgcaa ctcttgagtt
     2821 gcattttata tggtactgaa aatctagtca agataggtta tccaaccaaa tggatcggga
     2881 atatcgccaa actggattcc taccagggtc tcatatagtt ttgttgaaat cggtcccggt
     2941 tttccgtctt tgcaaaattt atatacatgt cctgaatcaa gatcattaat ttctccaata
     3001 ggtgaaataa cagctgcagt accgcatgcg cccacttctt cgaagctgtt aagttcctct
     3061 tcaggaaccg gacggcgctc ggttttataa ccaagatgtt ctgcgagctg aatcaggctc
     3121 ttgttcgtta ttgaaggcag aatcgaagta ctgctgggag taatgtatgt gttgttttta
     3181 atcccaaaga aattggcagc accaatttca tcgatgtatt ttttctcttt ggcatcgagg
     3241 tacatcggcg agccataacc ggccttatga acacgttctc cgcctttaag acttgcagca
     3301 taattcccac cgactttaaa ggttcctgtc cctaatggag cagccctgtc ggagtcgcgg
     3361 acaatggcta ttttaatcgg gttaaagcct tctttaaaat acggaccaac cggtgttaca
     3421 aaaataagaa acatatattc atcagccggt ttaacaccaa ctcgcacacc gcttccaaag
     3481 agcaatggtc taatatacaa tgtggctcct gtcccataag gtggtacgaa ttctttgttc
     3541 cgtaaaactg ctgtttttac catctcgaaa aacaaatcct tcggtacagg ttccatcaga
     3601 ataccatctg ctgaattctg cagacgcctc cagttttctt cccaacgaaa caaccgtatt
     3661 ttaccatctt tgcccatata ggctttcatt ccttcaaacg cttcctgacc gtagtgcagg
     3721 gcagttgcag ccatatgaat attcaggaac tccgaatcgg ttatttcggg cttactccat
     3781 gcaccatttt tgaaatacgc ccttacgttg atattggttt ttacataccc gaaaggtagt
     3841 tgcgaccagt ttaattctac catagtatta agtttttcgg ttaacaaatc caaaaatatg
     3901 attattgatg aaacaatcct aatgttccga tatgttttgt accataatta acaattgtat
     3961 caggatgtga tggtattcag atatctttat ctttaattcc agcgctattt cttaatttct
     4021 taagccctgc ccttcctaga ggtgtcttgc taaaacactc cttaaagttc tcttcattca
     4081 tatttaccag ttcatcgtaa ctcaggtctt gaacagcatt ctcagggata aaaaaccttg
     4141 aatccatttg tattttgttg tacgggcata cttcctgaca aatatcacag ccaaaccaaa
     4201 gctgtggagc cctggctctt atttcttccg gaacagattg cttgttttcg atggtatgaa
     4261 atgagataca tttctccgga ttaagctggt aatctcttaa ggcactggtc gggcacgcat
     4321 caacacataa cgaacaatca ccgcatgagt agggaacagg tgtgtcgtat tccacttttt
     4381 tgttaatcag aatttcgcac aaaaatccaa atgaacccaa gcggggatga ataatacaag
     4441 tgtttttccc ttgaaaccca attcccgaca acactgcaaa atgcttttcc ataaccgggc
     4501 cactatcaac aaaaactctg ccttcaacac tgttgtcgta ttcttgcagc tttttcagaa
     4561 gtttatatgc ttggtttttt aacaccagat ggtaatccga tgagtgggca tagcgggcta
     4621 tatgtacgtt ttttttcgaa tcaggaataa aagaataata cgcaaaaaga gaaatcaccg
     4681 acagagcgtt ttccaccaac aaatctggag cgaaccgaag ccgcaaattc ttccggatat
     4741 attccaatgc ttgatagccg ggtaaggaaa tcgctttttc gtattttacc ttaatagaat
     4801 caggaacaaa tgccgatgaa atgccacagc catcgaaccc cagcatatgg gcagcttttt
     4861 taattgtatc gtttgtcagg tgatccgctg ccacggataa tattagttat ttgagtaggt
     4921 attcccgttc cagtatatat tttgcgattg aaccccaaac gaatatctta cacagtttcc
     4981 tgatatctca aaatcatcaa ttttttcgta agaaattgtt ttggtcacgc cattttgaaa
     5041 cacctttaaa tatccatgca catcgatgta agccaccatg tcgttatttg cgatgaatcg
     5101 ctccgggact acattttcaa gtgtataaga tttctcacga taaaacactt taaagtcgtt
     5161 ctgtaccgaa aaaaccacca attcatcggt tacttcatag aaattgggtg tatcgaaact
     5221 gattgtaaac agttcaccat ttgcgaaaac tttcagataa ttatcagcat caacataagc
     5281 caaaagatta tcgccacaat ggtatgataa tggctcaaat gattctaatt caataatttc
     5341 atcgttataa taaatttgaa aactgttaat gggttcttca acaaatgcca caatatcacg
     5401 gccggcttct accgatttaa ttcgctcaca attagcaatg ggtataatct tttcatcgta
     5461 gaagaggtaa aaattgtttt tactgtcgca aaatactatg gtgttttcgc cgcacttgaa
     5521 attgcttaat gaatcaagat tcagggcgtc gtctagttca aagaattgtg agttataata
     5581 ggcaatcagt ttatgtttca ggtcatcaaa aaatacaatt acatcatcac cggcagcaaa
     5641 accgctaatg ttggaactca ggttttttat ctgaccatta tcgaaaactt ttaactgtgt
     5701 attcattcgg agaacaacca gattatcggt aacctgatat tccgttgcaa aagcacttac
     5761 actgaagaga tagttattgt agaaaacctt gaaattcccg gtattatcct cataggccat
     5821 ggcattgttc cctattttgt atgatttcag gggcaaatgc tctatctgcc tgtggttccc
     5881 gctgtcgaaa acatacactt tgtcaaggta atcgctataa accgaaatat gctgagcctg
     5941 caatattgat gcactcatca acaaaagcat tgctccagac aaaatgtgca tgacaaagaa
     6001 aaagggcggt ttcataagtc tgttttatag caaataacaa atataaacat tttattcatt
     6061 aaataaatga tggtattttt gcgcagaaca attgcaaata gtttaccaaa aattcttttc
     6121 atgaaacaaa accaatctgt cgaaagtatt gaaagcttgg gtgaatttgg actgatcgaa
     6181 taccttacca aagattttat aacacactgt aattcaactg ttaaaggagt tggcgacgat
     6241 gccgcagtta tcgattgcgg cgattatttt cgtttgatta ctaccgactt gttggttgaa
     6301 ggaatacact ttgaccttat ttacacacct ttgcgtcatc taggttataa agcggtaaca
     6361 gtaaacctga gcgatatatt tgccatgaac ggtaaaccgg agcaaattac tgtttcaatt
     6421 gccctgagca acaaaatatc gcttaaagcg attgaagaaa tttatgaagg cattcggctt
     6481 gcctgcaatg cgtatgaagt tgatttggta gggggagata caacaacatc gctaaccgga
     6541 ttattgattt caataaccgc cattggcact gcacgaaaac aggaagtatg tttacgttcg
     6601 ggagcgcagg tgaacgatgt tatttgtgtc tcaggcaatc taggagcatc gtatcttggt
     6661 ttgcaactgc tcgaaagaga aaagcagatt tttattgccg acccgcacat tcagccagaa
     6721 attggtaaat acccatattg cattcagcga caattgaaac ctgaagccag aaaagacgtg
     6781 gtcgagaacc ttaaaaaggc agacattaat ccgcatgcta tgattgatgt ttccgacggg
     6841 ttgtcaagcg atttgcttca catttgtaag caatcgaaag tttcgtgcaa agtgttcgaa
     6901 gataaattgc ccatcgatac cgaaacagcc tctcttgctt gtgagctgaa catagaccca
     6961 accattgcgg caatgaatgg tggtgaagat tatgaattgc tttttactat cgatagtaag
     7021 gaacttgaaa aaattgctaa aatcgacggg ataactgcaa tcggatatgt tacatcagcc
     7081 aataatcgcc ctttgttaat tgcacgcaat ggtgttgaaa tagaactgaa ggctcagggc
     7141 tggaatcatt ttgataaata acgcaatact gattttcaaa aagtagtttt taccatttta
     7201 aaactgctta ttatttgact attttctcgg agatagacaa tatatagtcc atcgttaaca
     7261 acatttgcgt cccaaacata gcggtaatat ccttcgtttt cgtagaaatc ttctttgcaa
     7321 aacacaatgc tcccggtgat atcggtaatt gtaattgaag tattactttg agcagaggag
     7381 tgatacacaa tagttgccgt ttcgctgcaa ggattgggaa agcaacgaag catagtattt
     7441 acaggaatcg ggttaattgt tgtatcgctt gactgatttt ccttacaagg agttccgtaa
     7501 tacttaactg cgttccagtt tctacccaac gagttatcca gcagcggaga attaagtgcc
     7561 aaaacccgtc cgtctccatc ggcttcttca ggccagggaa tgctgtcatc gtaaaccaat
     7621 aagtccacaa gtctgccata gctattgtat aatctcaaaa gttcccctga gttgtcgaga
     7681 gcgaaattaa aaacgtcaat attgcactca ctggcaggga acagttgtag gaacttctgc
     7741 ggctcatttg ctagaatata gtacgaccct gaaggaataa ccatccccgt tgggaatgta
     7801 tacctgtgca gatcattgtt gtcgcagaaa taccagtttg aaacatttac gtcatatgcc
     7861 gaggcattgt aaagctcaac ccactcgccc ggatcaacat ctgaatcggg gtgataatta
     7921 atttcgttaa taattatttg agaagtatcg ttgtcaacaa aaaaaatatt atagttatca
     7981 aagccatcaa gatgatagtt ttgtgtgtat gcaaccactt tgtaataata tttcccgggc
     8041 tcaatagtat cgcttagaat taataatgtg tcggagatat tttcaatcat gtttgccgtt
     8101 ccgtcaacaa gattaatatt ttttccgtaa tatagtctgt actttatctc attccctaat
     8161 ggatcttcac attttgtcca cctgaacaca atgggttcac cggtaaaata cctgttgttt
     8221 actctatcca cgcgaaaaga acgggggatt tgctcaaact gaaccatgag ctcatagtat
     8281 cttgaatcaa aggcagtttt aactgtgttg atttgcgaaa gccagtccga aatagtgttg
     8341 atgttgtcgg tcgtgtcatg ttctactgaa ttataaagca acaattgcag actgtcgacc
     8401 cagggctcca catgttcgtt gtttataagc gtggaatgca actcggtgat acgctcccgg
     8461 atgtcttgca aagtggaatc gttcagaaat gcaagttcag caaagggatt gtcaggagtc
     8521 cagtatctag atgtatgata atagggtata ttgacttcgt aatataaaaa actcttgtcg
     8581 agatcccatg gaaatatgtg ccatttccgc gtggaataag agtcgttgaa catatagtaa
     8641 ttatggtaat atgtgctgtt gttgcgtatt aatagattta ccgctaaaaa attaatcagg
     8701 ttttggtaat taaattctgt aaatgcgaaa gaataataat cgcttatact tacagtatca
     8761 agctggttaa taagcttttg taaatcggag taatcgctgt tccccgactt tttctcccaa
     8821 tgatagaaca cattatcgta acgactgagg catgcaccat caacggtggc tttatagaga
     8881 ttgccggatg ggttaaagcc gtttcgggta agaaaatcct catccatatt ttcaacatac
     8941 aaatacagtc ccaggtattc tccgttcaga tacagcctta catgctccgt tcggaaacac
     9001 cagagccctg attcctgcat catccgcgag caaatatact gttgtaaata acttttgtct
     9061 tcgtattccg cattaaaatt aaacacacga attccatcat cgaaacaaag tgagtcaatt
     9121 tgaattttta gtgacttttt gggtaaaact actgtgccat cgccacgaat acgcattctg
     9181 gcttttgtaa gtgttacatt gttatgtgta atacttatcg gtatgtaaat ttcttcgttg
     9241 tagttttcat aaatcattct gaagtcatcg gggttgcaac ttatataata ctctttaagc
     9301 tcctgggtaa atgctgcttt ttcgcgggag agtaaaaaca ccgaaatcag taaaagaaaa
     9361 tatggtattc ttttaatcaa gctttagaaa ttttgaagaa aacactttcg tgggagtcac
     9421 aacaacaagt tgataaagtc cgggttttaa aaagctgaca ttttgctcaa tcttatttat
     9481 accagtatca aaatgaattg tgcgatttaa cagtatcttt ccctccatgt taagaatgga
     9541 taaatctcca aattctgggt aaggagcatc aaaatcaaat acagctacag tatttacagg
     9601 gtttggatat aattgaacac tggtgtgttg cataatctgc ccaatcattg tcagactgct
     9661 tatcaggccc atatccaacc cgtcgtcggc agctccgctt ccgggagagc caatttcaag
     9721 tagataggct tgcgaaaact gaggagtacc tgcgatgcat gagttttgac catcaaaatc
     9781 aatgtcgttt gtaatggaat acataacttc aagcgagtcg gtaacaggcc cgcctgtctg
     9841 tggttcccaa ttcagcaaat tatcggtgtt gtcaaaaaaa atactgtttt taattatcat
     9901 tttcccctca ctgggttgaa cataatcatc cccgaaacgt gcacctacgc cattatgagc
     9961 aaaaacacaa tgatttgcca taacatgcgg cgacccgtat ccagcctcaa tcccttgtcc
    10021 gcaatcaacc acaacagaat tatgcagcga aacataattt ccgtttgatc cagcaatacc
    10081 ttcatggtag aaatcctgaa tccagcagtt ttccaaactc agctttgctc cattatggtc
    10141 gatggcatcg tcttcacccg taataaaaaa cgaattcgaa accttgcttt ctgaattcga
    10201 agaatgtaca ttgttaaaat aaaaacaatc gttgtcatcg tcttcaatta tcccatcgtc
    10261 atcgggaatg ttcataacat agcaactgtc gatatcaacc tttgacataa aaaattcacc
    10321 accggtgtcg catctggaga tcagacattg cttcagactg atatttgcat aattaccacc
    10381 cagagctttc cccgtgttgt cgatgaagta gcaatcccta caaatcatgt tcccattgtc
    10441 ggcttttaaa acaggctgag aaccagaatg tccgaaaatg aaattggtgt ttcctccgcc
    10501 cttaataaaa aaaacatgct caaatactga agtgtcctga gcagaagtaa ttgtaatacc
    10561 accccagaat atattttcac tatcactttt aaaaacaatg ggattctcgg tatttgcgtc
    10621 acatattatt ttaccatgac agattatgtt tgcattttca gctacaatca gctgtgctcc
    10681 ttcgtaaata gtgagtgttt gagaagcttg aatggtcagg tcggagctta cgtaatacaa
    10741 tgtatttgca atgcattggt aatcagaatt tatatatccc gatatttcaa ttgggtcgtt
    10801 aatggtatga tttacattct tgatggtact gaaaccctgc acttcaacct gaaagtcggc
    10861 acttgtgttt atttttgtgc aaaggcttcc tactcctttt ttaacaacaa tcatcgagct
    10921 ctctgcagtg gcattggtta cagcaagcga gtagctatgc ccggcatagt tcaatgcacc
    10981 attcttatat aagcgaacta ctacggggaa tatatcattt gaggttgatt gcgaagggca
    11041 aattattaca gcgctgtcgg gcgtttgcga tattattaca ttcgcccaga aaatgaaaaa
    11101 tactattgaa atgcattttt tcatagttcg cggaattgac actgtgtaaa attagtgtaa
    11161 ttaaaaagaa ttttgttaaa aaataggggt ataaaagaaa acttacgaag aaatcaaaga
    11221 atcaacacgt atttttattc tatatttcca aaatatcctt tgataatacc gcgtttgctg
    11281 tttctgataa aaagaaggat ttcatccctt tctttcgatg cggtcatttc agcttcgata
    11341 aaatcaactg actgcgtcgt gttcataccg cgaccaaaaa ttatacgata gatattttgt
    11401 agttcattta ttttctcgtt atcgaaattc cggcggcgca gtccaataga gttaactcct
    11461 tcatacgata aggggtcgcg gcctgcctta acataagggg ggacatcttt gccaactttg
    11521 cttgctccct gtatcatggt gtgtgcacca atattcacaa actgatgaac cagactaccg
    11581 gcagatacaa tagcccattc gtcgatgatt acttcgccgg cgagagccac ataggcacct
    11641 agaatcacat tgtccttaat aatgcagtcg tgtgctacat gagaataaca cataagcaga
    11701 caattgctgc caataactgt tttacccttg gcaatggttc cgcggtttac cgtaacacat
    11761 tccctgatag aagtgttatc gccaatttca actgtggtta tctcgccccg gaatttcaaa
    11821 tcctgaggaa ttgccgaaat cacagctcct ggaaaaattt tacagttttt accgatacgg
    11881 gcaccttcca taatggttac attcgatcca atccatgtgc cttcaccaat taccacatct
    11941 tttgcaatgg taacaaaggg ttcaattacc acattttgag caattctggc ttccggatga
    12001 acatatgcta aaggttgatt catagtgtta cttttgtttt tcgattaaag cgtgcatttc
    12061 ggcttctgca acaagatcct tgccaacaaa cgattctcct ctcatgtgtg caattcccct
    12121 gcgcatcggc tccaacagtg taagtttcat aataaggata tccccaggta ctactttttt
    12181 tcgaaattta acattgttga ttttcaaaaa cagcgtagtg taattctctg ggtccggcat
    12241 agaatgcaac acaagtatcc cacccgtttg tgccattgct tctatctgca atactccggg
    12301 cataacaggt tctccgggga agtgtccctg aaaaaaatat tctccgttcg taatgttttt
    12361 aatacctacg acctcggtat cgctaatgct gataattttg tcaacaaata agaagggaga
    12421 tctgtgaggt aggtacgtct taattttatt tacatccatt accggtttaa catccgcatt
    12481 gtattccgga atccggcttt ctttttcgtt aatagtggat tgttgtaaaa tatgatttct
    12541 gataattttt gccatctgag tgtttccata atgcccgggt cgcgaagcaa caatggtggc
    12601 attgaaccta aaccctgtta gagcaaggtc tcctacaacg tcaagcaatt tatgtcttgc
    12661 aggttcgttc gggaaatgaa gtacaagatt gttgagtatg cccaatgatt gcactttgac
    12721 tttgggttta ttgaatagct ttgtcagacg gtcatattct tcctgtgtcc actctctgtc
    12781 cacaatcaca atagcattat cgagatctcc tccttttatc agattcagac gaagcaatgg
    12841 ctccaggtcg ctgataaata caaatgtcct gcatttgcta atctgatttg taaaatcttc
    12901 gagactgtcg agattcgcat actggtttgc aagaacttcg gaattatagt cgacaagaac
    12961 ttttattttg taactactgt cggggtatgc tgagatatcg acccctcttt ctttatcgga
    13021 aaatgagagg gtttccgtta aattgaatac tttttggaag gcattttgtt ctttaatgcc
    13081 ggccttatag aatgcctcaa caaagtaatg ggaacttccg tcgagaatcg gcacttctgg
    13141 gccgttcagc tcaaagatgg cattatcaat cccaagagaa taagctgctg cgagcagatg
    13201 ctcaaccgta ctgacagaag tgccattatt actgatggtt gtacctcttg aagtgtcggt
    13261 aacattatct gcaatagctt ttacacttgc ttccggtgaa atgtcggttc taataaaaac
    13321 ataacctgta tcaggttgtg cgggtttaat taccagcttt gtttcaactc cagaatgcag
    13381 accttgaccc tgcaatacaa ctgttttatt aacggttttt tgttttctgt tttgttctgc
    13441 cattaataat agctcttatt aattggcaaa tatagtatta ttgattaata aacgaacaca
    13501 tgtttattaa aatgagtgaa accggctcag ttctgatttc ttaagaaatt gcatagaagt
    13561 tagcagtcgg ttttttctgt tgcaaaaaaa aatatgtatc gcgtgattag tacgatttta
    13621 aaaatgggga attatttttc tttatccttt atatgttttt ccagttggtt gagtcgggca
    13681 agcatatcgg gcaagtgcct gaaaacagca aaacaacggt ggtattcgtg cccttccatg
    13741 gcgggagcac caagcatttt tttataatct tcaacgtcgt gactaacccc agttttagct
    13801 ccgaccatta caccgtttcc tactttaatg tggcctgcaa aaccagattg acctccaatc
    13861 atgcagttct taccgatttt aactgtacct gaaacccccg ataatgcggc cacaaccgtt
    13921 gaatctccaa tttcagcatt gtggcctacc tgaataagat tatcaagttt aacgccttta
    13981 tgaatacatg ttgaacccat agtagctctg tcgatacagg tgtttgctcc gatttctaca
    14041 ttatcttcaa tgatcacatt tcctacttgt ttaactttaa taaattcaga atcgctttgc
    14101 ggaacaaatc caaagccatc ggctcctaca acagtacccg aatgaatggt acaatgctgc
    14161 ccaactacta tgtcatgata aattttaaca cctgaaaaaa gacaagtttt ttgtccaatt
    14221 ttgctgtttt tacctacata aacatgtggg taaatttgcg cgccatcccc gataacgacc
    14281 cctttttcga tgcaggcaaa ggctccgata taaacatttt tacctattgt tgcttcagaa
    14341 tgaataaaag ccatgggatg tatctcttcc ggctgagaag tttcggtatt gtagagctcc
    14401 agaagtttcg caagggccag atatgagttt tctaccttta tcagtgtggc agaaatgggt
    14461 ttttccggtt caaaactatt gttcaccaaa actatgcttg ctttggtact ataaatgaat
    14521 ggagtatatt taggattggc cagaaaacta agtgtgcctg cttctccttc ttcaattttc
    14581 gaaatgttac tgacttttat atcgggattt ccaactacct gtcctcccag tatttgggct
    14641 aattgttttg cgctaaactc cataatatag tgtttttttg taaagttacg gttggttaat
    14701 tacgtgttga atttcggttg gaaaacaaag tatatgtttc cttactgttt tactcattac
    14761 agaatggtta agcatatcgc ttgcttcagt aacatcaaca aggcgtccct gaacaagaat
    14821 attgattttt tcatcagcaa agctgtaagc gttgttcgaa acagcgtccg taaagacaag
    14881 atgagaagcc tcgttaatac caatattaaa atgtttcgcc gcctgttttc gtaatttctc
    14941 gatgtaatca gaactgaaag gctctttttc cagctttact ttaaacaaat gacggtcgat
    15001 tatacccgac gataggtatt gaagaagaaa atcatcatga tctgtccatt tctttaacgc
    15061 gtacattaca tcataatcgt cggttttcgt aaaatgagca agcatttcag gggaaatctc
    15121 aaaatgttta agaattaaga aataggatag tggttcagag caatcagcat gaattcctga
    15181 ttgcaccaat tggcgcgctc tcccgatgca tttcgttagc attagttcgg atgcaagtac
    15241 agttttatgg agatatacct gccagtacat aagccttcgt gcaataagga atttttcgat
    15301 actgtatatg cctttggctt ctacaaccag ctcatcatct accacttcaa gcatttttat
    15361 tatcctgtcg ctacttacga ccccctctga tacacctgtg aaaaaactgt cacgccgaag
    15421 atagtcgagc cggtccatgt cgagctgacc cgataccagt tggtggagaa attttttgtg
    15481 gtatttccct tgaaaaagat cgattgcaag cgataatcgt ccattgaaaa cagtgttgag
    15541 ctcattcata atggcaagtg aaagctcttc gtgcgaaata cctggaataa tgcagtattc
    15601 aagcgcatgt gagtacgggc catgcccaat atcatgcaac aaaatagcaa taagtgcagc
    15661 ttcggcttct tcatcggaaa taacatggcc tttctgtcgg agcacttcaa tggcaagagt
    15721 cgttaggtgc atcgctccta aagcatgctg aaaacgggtg tgaaaagctc ccgggtacac
    15781 caggttggtg agaccgagtt gctttattcg gcgcaatcgt tgaaaatagg gatgctcaat
    15841 aagatcatat acaatgctgt ttgggatatt gataaaccca tataccggat cgttgacgat
    15901 ttttctttta ttcaatttct ggccaccttg attgtttgtt gcatcaaaaa taaaaaaaat
    15961 accctgataa aatatctatg aagttttttc ccattcgttt ctttctgtat ttttatgcgt
    16021 aacaaaatga ttgtattaag ttgcagtggc ataaaaattg tattttttaa gtcttaacaa
    16081 aatgattatg agaaatttat tgctcctcgc tctgattact ctgttgtgga gtacttctgc
    16141 tttttcgcag aatgatgaag atttcgataa atttaaaaga gaatattata acggactgga
    16201 ggatttacga aacgattaca agagatttgt tgagcagaac gataaggaat ttgcagaata
    16261 tatccagaaa gagtggcagc aattccaact atttaaagct gagtatgcaa gtcctgcccc
    16321 gggtcctgat gttgtccctg tttacaaaaa actgatcaat gttgaacctg taaggcgtat
    16381 tcagccttca cagatacagt tgcagactaa aaccatagaa atggcggttg aacccatgaa
    16441 tccaattccc gcaatcactt ctgcagacaa ggaccttcgg gataacaaca tttgctttga
    16501 tttctacggg acacatcttt gtattcctgt gcctgatgat atgaggagta cgttttctgc
    16561 ctcggttgat gaacaaaact ttgcttcata ttggaaaaaa atgagcgatt ccggttttga
    16621 tgctcctatt gttgaattgc tgagggtaaa aagtaattta aatctgaacg actatgccat
    16681 gtatttgctg gttaatgaac tgggtacccg tgcaacaaaa gatagaaatt cagcaacatt
    16741 gttttcttgg tttgtactaa cgaaaatggg atataaaatc agactggcgt atcattcaaa
    16801 aggcgtttcg ctgcttgttc cggtttttaa tcaattgtat ggattcagct atctgacttt
    16861 tggaggagta aagtactata tgctcgatgg aacaaccggt gctgtgaaaa cttatgaaaa
    16921 agactaccct gcagctaacc aaattatgaa ttttaatttg tacaaacccc cttcttttga
    16981 cgtagatacg cttactagaa acatcggttt ttcttttgac tcgaaacaat ataatattaa
    17041 agtgctctac aataaaaacc tgatagactt ctacagtagc tatccacagt gctatattgg
    17101 gttgtatttc gatgctggag tatctgaaat aactaaagag tcgctagcaa ctgagctctg
    17161 gcctattatg aagggtatgc ctgaaaaaga tgctgcatca tttctgctaa aaatggtaca
    17221 aacagggttt tcatacaaaa ccgatcagga gcaattcggc aaggaaaaat attttttccc
    17281 tgaagaaata ctccactatg aagcatgcga ctgcgaagac cgctctgtgt tctttgccta
    17341 tatcatcaac gagcttctgg atttaccggt tatcagcctg aattaccctc aacatgtttc
    17401 aactgcagtt tgttttacct caatggtaaa tggagacctg attcaatatg ataatcgtcg
    17461 atttactgtt tgcgatccaa cctatatcaa tgcacctgta ggagctacaa tgcctcagtt
    17521 taaaaaatcg aaattcagta ttatttcacc ctcaaaaaga tcgaatcaag atgaacagga
    17581 agaatttgtc tgggagaaga tttacaaagc gggcgggttt aaagggtccg acaaaactat
    17641 acttatcagc gaagacagct cttgtttaat tgccggtttt tttaccgata cgcttacaat
    17701 gaacggtagg tcttgggttg ttgagaatga gaacacaggg gtttttgttg cccggctcga
    17761 taaatacaac aaaccgttgt gggtatcaac ttttgctaca gatggcagca tatcgctgca
    17821 aaacatcatt atcagttccg accggagtgt ttatgttagc ggagagtttg ggaaaaactt
    17881 gtattttgat aagaagaagc ttgtatcttc gtcggtggcc gatcaatttg caattaaatt
    17941 taataaagac ggagagcttg aatggcaaac ttctatacgc ctcgattcga tttatggtgg
    18001 aaaacccttt tttctgcagg taacgatttc ggatcgcggt gatgtaaaag gccttacttt
    18061 tgttgaggaa ttcagttata accctgagca aaccttattc ctgaatacag aaaacgattt
    18121 ggtgataatt gctgaacgcg gtgtttccga atcaggattg aaaatcagcc cggaacttgg
    18181 actgtctggg aattacgagc tgttggaaac atggaaaaag ctcacagata aatacatttc
    18241 gatgcattac gataaaagta ttgctggttt ttttgcacta atggaaacct tacagaactc
    18301 aagcctcgac attagtggga aagaaatgat ggcaagtctg aatacattaa acccttcgtt
    18361 taaaaataat tataaaaact tctatagtag tttacagtat ttaacccgaa tttcgagccg
    18421 taatggaatt gtaacatttt ataccagcgg aggtaaggtg ttgaaatgcg ggccgctgag
    18481 cattgtaaac ggtgcaagag cgcaaatgcg tgactataaa agcggtaaca aacaggtcaa
    18541 ggtcctgaac ggtatttatt acgacagttt tttcagaagc ttgcgggtga actatcttaa
    18601 atgttttaag gtgaatggcg atataatgat tgattacgat aatgagcatt atcagaaagt
    18661 ggtaaatact caaacagata ttctgaagca atgatttagt tttttacaat atgttttata
    18721 attttacaaa caatttaaca tccgctgata catgagaaat gcatacccaa ttgcagcagc
    18781 ttttctgata attttaattt cggtttcgtg tgcttcttcg cgtcttacca agaaaggttc
    18841 aaaaatggaa gaagccggaa tgtatgtcga tgcagcagaa tattattatc aggcagttct
    18901 tgcaaaaaaa tcgaatgtcg atgcgaagct cggtttaaaa cgaaccgggc aaagagtgct
    18961 cgataaaaaa ttatccgcat ttaacaaatc gtataatgag gaaaaaaatc aggaagcagt
    19021 gtataatttt aaagatgccg aagactatta caataaaatt accgcagtag gtgttgagct
    19081 gaatttccct tcatattacc gcgaaaatta cgacgaagtc aaaaatgttt acattgccga
    19141 taaatacaac gaagcttcaa agttgctcga aaacgagaaa tttgctgaag cagagaagct
    19201 tttccgtgaa atattgcaac tgcaaccggg ctataaagac tcaaaggaaa agctgaatgt
    19261 tgcaatttat gaacctgttt acagagacgc acagcaaaaa atgaacaaca agatgtatcg
    19321 tgcagcatat tacttgttcg ataaagtgat ttccggctca ggcaactata aggatgcata
    19381 tgaattaaaa gcggaatgcc tcgagaaagc cacacttatg ctggctgttt caccggttgt
    19441 taacaaatcg ggtacagcag gaattgaaac cagtttgcaa gctgcaatta ttaaagacat
    19501 ccaggataaa agaaatccgt ttttaaaact ggttgaagcc aatccccgac tccagcctga
    19561 cgggtcacta agtaaaggac agcaacctcc cgttaaagca gtaaacccag acataacact
    19621 ttacactaca gttacgggtt ttacctacga aaaaggtatg ttaaaggaaa ccgaaaagcg
    19681 tgggtatcga aaatacacaa aacaaaccaa ggatgaagct ggtaatgtga gaacagttac
    19741 tgattacgaa aaggttactt ataaggagta ctctatgggc cgacaggtta aaatttcgtt
    19801 tacctataaa ctgatatata acaaaacggg agagataatt gccacaaata gtcacacctt
    19861 ggttagcgaa gataaaattt tatatgcaga ctataccggc gataagaaag ctctggtacc
    19921 tggttactgg aaaaataaag caggtcatag ctccgaagac gtagtcaacg ataaccagaa
    19981 agatgtgaaa gcattgcagg aacttcttga cggtcgccga gttattaagg actataacac
    20041 tctcaccggc gaagtgattt cagcatcatc gatgaaagtg gccgatgcgg ttaatgaata
    20101 taaccccgaa gaataggcat gaaacaaacg gtaatactat ttttacttgc cggtttgttg
    20161 atgagcggtt gcaagaccct gcaaaacact acagaggcag ttaaaccaca gcctgaatgg
    20221 gtaaagtcca aacctgtaag ttcattttac tacataggaa tcggctctgc aacaaagcca
    20281 gggtttgccc ctgccgatta tacccagaca gcgcagcaaa aggctcttgg cgacctggca
    20341 agggagattt cagtaaccat ttcaagtgca tcagttcttt cggtgatgga gcataattat
    20401 aacattaccg aaaactggac atcggaagta accacttcaa ccgaaacgta ccttgagggg
    20461 tatgaattgg ttgattcatg ggaagataaa aattattact gggtatatta ccgcctttcg
    20521 aaagaaaagt atgcacaaat gcaggctgag cgaaaaaata aagcaattgc cgatgcagta
    20581 tataagtact acgaagggca aacccaacgt aaggcaaact gctataccga agctttaagg
    20641 ttttatgccg atgctcttac tgcgctgaaa ccttatatga acgaatcaac agagacaatt
    20701 gttgacagcg ttaaagccga tatcgggcaa acggttttaa atgcaatggt atcgatgata
    20761 cagtcgatag taattgaaca cccttatacc gaaatcaaag taatacgcgg aacctctctt
    20821 tctcaaaccg aaaggagttt tattgtttat aaccagaaca ggcaggtaat cgcggatttg
    20881 cctgtggaaa taaaatatac aggagtggag cttaaaaatg aacgggctgt cagcgataac
    20941 tccggaaaag taatgtgtga ttttaataaa atagttagta gcggtgaaac agaaaccata
    21001 agtctatctg ttgatctcat aacgatgtcg agagtttcga aagacccgtt tattcgaaaa
    21061 ataatcaaac aattgccggt aaaaagaaca tcagtagtac taataattga aaaaccggca
    21121 ttctttgtaa taagcactga aaaggaattt ggcactgaaa cagggaaagc tttactttcc
    21181 gatgctttaa gacctgaaat ttcccgtgca aacaaaattg ctgatacaca atcgcaagct
    21241 gattttgttg ttcaaatcga cgcaaatacg gtagttcgag aaaaagtggg ctacgaaaca
    21301 atcactgata tcaatgttat ggtaacggtg ttcgacaaga aaaatgagca aaaattccag
    21361 aaacaatatg tggccgaggg tgaagggcag aatagtaagg aatcttcaaa tgatgcctat
    21421 caggaggctg ttcgtttaat aacccgtaag gccataaatg atattaattc agcgttgttc
    21481 agtaatttac cttaattctt catgtatgtc agtcctgatg agcctggtga ttttcccaac
    21541 cgataaaggg agcagtgtaa gcaaatacgt aagcagggtt gtagaaataa ttcgtgatac
    21601 agggtatgat taccgtctta cacctatggc tactattatt gaaactccaa gccttaatca
    21661 ttgcaccgaa ataattgaaa aagcttactc tgctattgga gctgacagtg aaagggtata
    21721 tattaccgca acattcgata tcagaacaaa tggaacagaa caacgcttga attcaaaaat
    21781 agcctcggtg gagtcgaaaa taggagatgt gaaaaaataa atacatagaa attaatcaga
    21841 aaattgatta aatttataac ctgatgaaca aattgctaaa cctgtgtata ttttttttcg
    21901 tataacattg taaattgctc ttcaagaatt aaaataataa atatggtttc ggttaacgat
    21961 ttatttggtt gtttgactaa agttgaaaac ctttggtgtc ttggtgagta tggcgttaga
    22021 aatacctgta tactcgagtc gcttgataca tttccgggtt attacggtaa tgcaccaaca
    22081 acctctaagc cgttgtatgt atatattgtt gcaggagagc actacactat ggaggatata
    22141 gtgagaattt cgctgaaagt gaaagccgac cataaaagtc cattcgatgc ggcgtttaca
    22201 gaaatctcga tgcggcacgc tacttgttgt gctgtgcgaa ttgcaggtgt tacaaactac
    22261 gataacgtca aagaattaca ggaatgtttt cagcaccatg ggctagttct taaaaagaaa
    22321 gttaacaata ttgagaactc tcccgccctg attaaaattt ataagttctt tcttcttgat
    22381 ccggttgggg ataatatttt tcttgacctt agaaaccccg atattgggta tttcgatgtg
    22441 tatgaaaatc ctgcatggga cgagttttca tcacgtattc aggtattgag gaacaatatc
    22501 tccggacaat cgtttgatgc tgctaaaagt tttgtattca aaggagccga aattcaggat
    22561 atggttcgca tctactgcaa aaatttaact cttgagtttc ttaaggggtt gaaagagaaa
    22621 tactacctgc tctttgcgaa tggccggtaa gggattatta taggtactca cttttattgt
    22681 ccagtgaaac aaatggtgcg actgctcttt tgtaaatacc ctgcatccat gctattgcca
    22741 ttccgtaatt tgtaactggt acacctgctt ccacagcagg tcttaaccgg ttgattacct
    22801 gttttcgtgt tagtacacat ccaccgcact gtacaactaa tgcgtaatcg cgaatgtcgc
    22861 gtggtaattt atcaagtccg gttacaacat cgaaatcgag cttctttccg gtatagttgg
    22921 acatccatcg cggtattttc accctgccaa tatcatcaca cgaaacatgg tgagtacagc
    22981 tttccaaaat gagaaccctg tcgccagtcc gaagttccga aatcttgggt gtgcccgcaa
    23041 ggtagtttgt aaaatcgcct ttatagcgag caagcagaac actgaaacct gttagaggaa
    23101 tttcttttgg aatacttgca tcagctttta gaaaaacctg tgaatcggtt attgctaatg
    23161 cgggtttaat gccggttttt cgtaaaaaaa cgtccacttc ccgttctttt acaacaatcg
    23221 ctatgcagtc gttatcaaga atgtcgcgga ttgcctgaac ttgtggcagg atcattctac
    23281 cttcgggtgc ttctatgtcg attggagtta taagcaacac aatatcacca taactgatta
    23341 aatcgccaag taatgattga attttccacg ataaatcagg aatgttgttt ttaattgcat
    23401 caatgagtgg ctgaaggttg ttgtcggtta aagtgctgaa ttcgataata tcggtgctat
    23461 aatcattttt aagacttgtt ttgaatgtag gatcaagcgg ccgtaaatcc gatttattat
    23521 gtacgattat aaaagggaca tcgtgctgct taaaactata aatcagtttc ttctcgcttt
    23581 ccgaaataat attatttgtt attacaagaa ttgccaaatc gatacttgct attgaatgaa
    23641 gcgttttaga tactcttttc aacccaattt cgccaatgtc atcaatacca cctgtgtcga
    23701 taatgataac cggtccgaat cctgttattt cgatgctttt ttttaccgga tcggttgtgg
    23761 ttcccgcaaa atcagaaaca attgaaacct cttgccccgc aattcgattg ataagtgagc
    23821 ttttccctgc attgcggttt ccgtaaatac ctatatgggg tttgttttct ttgcctctgc
    23881 tcattatctt ttttgtttta ataaacaatt ataagaaaaa agaatacata atcgattaaa
    23941 aattattcgt aaattcgctt acttaataat gcgacaaccc tatatttaca gattaaataa
    24001 aacttagtga tattgtatag attattgaaa actattttta cgtcagcact tgtaattatt
    24061 tgtttttcaa gtgcattttc gcagcttgtt gaacacggac tgccgggttt ttacaattat
    24121 gcaccaaaag aatacaaaaa cgaaagtcag aatttttcga taacccaaga taaatttggc
    24181 tttctgtatt tcggcaacct taacagaata atccggttca ataatgttga atggcaaacg
    24241 gtacacctga ttggtacacc gatattggac gtctccgaaa atgggttgat ttatgctggt
    24301 ggatataaca aaatcggtgt cattaccccc tatatcaaca ggatggagat gaaggacctt
    24361 gctttgcctc ccagttgttc tcccgggcag atcaacaaag tcattgcttt taataatcat
    24421 gtgttgtttt tgggggataa tatgatattg gatattaagg acgaagttgt agaaaaggtt
    24481 tactcatccg aaaataaaat tcttgctttt aaggcaaata aaaagatata tgtttctatt
    24541 gagggtaatg gattgtttac ttatgatccg gataaaagaa ctatgcaacc tgtagccaag
    24601 ttgatattgc ctgaaaacag ggacgttatt gatttatttg ttttagatga aagaaacatt
    24661 cttattaaat gcgaaaatac cagcggattt tatctgttcg atggaacaac aacacttccc
    24721 ttcacaaccg acgctgatgc tttcatggag gaaaatcaat atataaaagg cataacttta
    24781 tcagatgggt ctattgttgt tggaacagaa tacggtggaa tcatttgcgt aagttcaacc
    24841 ggacagtacc ttttttcgat gaataaaaac cacgggatgc tcgataaccg gattactgat
    24901 ttatttgtaa gcaagaataa taaactttgg gttaccagtt ataacggaat tacttcagta
    24961 aattatccat ccccggttga ctatttcggg aacagctatg ggattaacgg cgcaattaaa
    25021 accatgattc gttacaatgg tacgatgtat tttggctcca ctcagggttt gtactactat
    25081 actaccacca acttggctaa atcaaaaaaa ctgataaaca acgaagacta ttttgccaaa
    25141 gttgaaggtg ttgttgccga gatacaggac ctgattatta tagacaatca attatacatt
    25201 attacttcaa aaggagtata taaacttatt cgagagggaa gagcaaggtt gtcgataccg
    25261 ggagagtttc gcaaacttaa agtcagcgaa gtctatgacg atattgtgtt ttgtattgga
    25321 gagcaaggta ttctggtact tcgtaaagaa ttagattcac tacatattgt tgggaaagtt
    25381 aaaaacttcg attatgaggt acgtactatg gtgcacgacg aaaaagatgt tttctggctg
    25441 gggtccgata atcatggctt actgaagttg gatttcagaa aaggagactt gttaaatcca
    25501 caaattcagc ggtttgacga aaataacgga ctgcccgaaa atttcacatg ggttgatgtg
    25561 tatcgtacta aagcaggtat tttgttcagt acccaatgtg gtgtttacta caacaatgcc
    25621 gggaaattta ttaggcaccc gttgtttgaa acccctgatt ccagcaatgt attttcattt
    25681 tatcccattg ttgagcaagc ggatggtaaa ttgtggtatt ccagtacaaa tactaaaaac
    25741 tccgaacgta aaacgggata tattacttac gacaaagact ttaatcctac cgatcacagt
    25801 aatgagttaa acaatctgaa agacaggaac atcgaaagta tttactgcga ttcaaataat
    25861 gtagtttggt ttggtggatt cgatgggctt atccgttatg aagccaataa aatggaaaca
    25921 tcaacaccac aggccttatg tttaatttat aataccgatt tgcctgattc aatcaacaat
    25981 aagctttttg atagaagtgg taaacagata ttgacacctg ttcttaaatc atctcaaaat
    26041 catattcggt tcgaatttac agcgttatac tacaacagct acaaggaaat aagctaccga
    26101 actcagttag tggggttgca ggatgaatgg tcgaaatgga gcggtgaaag ttttaaagaa
    26161 tacacatata ttcctccggg aacctatacg tttaaagtcc aggccagaga tattttcggt
    26221 aatactaccg agtcgcatgg ttttactttc tctgtgccta ttcctttgta tatgaggtgg
    26281 tgggcttttg ttatttattt tttatttctg atatccttta tctatctgat tcttaagcta
    26341 aatgagctga aacatgctac cgacaaaaac aaactggagc aaaccgttgc agaaagaact
    26401 gatgagttga tccgacaaaa agaacaaact caaaaacttg tacagaagct cttgcctgac
    26461 agaactgctg aagaaattca aaaagaaggg aaagctcaga cacaagcata tgaaagggtc
    26521 acagtattgt ttgctgatat tgaggggttt acaaaaattg ctgccaccac cgattccgaa
    26581 gtattgttta ctcaattgaa taaaatattc tcttcttttg ataatataat cgaaaaattt
    26641 gaaatcgata aaatcaaaac cattggtgat gcatatatgt gtgcaggtgg tatgagaaac
    26701 aacgacagaa ccaccccgat tgaagtggta atggcagcat taaaaatgca ggaggcgatt
    26761 caggaaatca acgaaacgga taaatgtaat ttcagaatac gtatcggtat tcatacgggt
    26821 tcagtaatag caggtgttgt aggtgctcag aaaattgaat acgatatatg gggcgataca
    26881 gtgaatgtcg caagcagaat ggaaagtaat gctataagcg gtacagtaaa tatttcttca
    26941 tcaaccttcg actatattaa agactttttt gaatgcgagt cccgtggaaa aaccgcggtg
    27001 aaatataaag gtgatctgga tatgtttact gtagtgaagt ataagcaaca cttgtcggag
    27061 ttctcaacag ggaaaacccc taataagctt ttctatacca aacttcaggc tcttcggtat
    27121 gtcgatttgg agaactacgt aatgaagaaa ctcgagacgg acttgccaaa aaatctgtac
    27181 tatcataact ctcgacatac gaccaatgtt tgcacccgtg tcgaaaacat cgggcgcgaa
    27241 gaaaaagtta ccgacgaaga actcctgttg ctaaaaacag ccgcattgtt tcatgatgcc
    27301 ggttttatga tttcttatga taacaatgaa cccctgggtg ctaatcttgc tgaggatatt
    27361 ttacctaaat accggtacac acctgagcag attcaaaaaa ttaaagcatt gattctgtca
    27421 accaaaatgc cccccaaacc gaaaaaccac cttgaagaaa taatttgcga tgctgacctt
    27481 gattatctcg gcaggactga tttcattgtt gtatcacaaa acttattcag agagctgtat
    27541 gaacgtggta aggtgaaaac aattgaagat tggaatcggt ttcaatataa attcattata
    27601 aaacacgaat attttacggc aactgcccgc aaattgagag aatccggtaa aaaagcagtt
    27661 attgaagagc ttaaaaagag aatttaatcc tcaaaatcat cggagctttc ttttagcgcg
    27721 ttataaacct ccatggccct tgcaaaatca gattctttta caagtaccat cacaccgtct
    27781 ataagtttaa aaaccgattc actggtcaat gcacactcta taccggcttc taaaagccta
    27841 tctttcagca aatatgccct gtattcgtat cggaacttgg caatcgttat taatttatct
    27901 tttatcattg cggtgtttta aaatttatac ttatttaatg gtaaaaaggt ttcataaaat
    27961 attaaaagat tcataataat ttttcttacc acttgtttta taaatttgag aaaaatttcg
    28021 aatatgcaaa agattgttga ttttttagtt attggatcgg gtattgctgg tttgagttat
    28081 gcattgaaag tgagtagaaa aggcagcgtt tgtcttgtta ccaaaaatgc acttgaagat
    28141 gggtcaacct tgtatgcaca aggaggaatc gcttctgtta catatcaacc cgactcttat
    28201 gaaaaacaca ttaatgatac cctgatagcc gggtgttacc tgaacaggga agatgttgtg
    28261 aaaatggtgg ttaacgaagg cccttcgcgg attgaggagc tgatacagtg gggagctcac
    28321 tttgataaaa acagagatgg tcgtttcgac cttgcgcgtg aaggtggtca tacagaatat
    28381 cgtgtattac atagtaaaga tagcacaggg cgagaaattc agcaggctct tattcaccag
    28441 gttaaactaa attctaatat tgaagttctc gataaccatt ttgcaattga tttgattact
    28501 cagcaccatc tcggatttct ggtgaaacgc atttacaatg atattgagtg ctatggtgca
    28561 tacgttcagg acaaacatac aggtaaaatt gtcacaatac tttcgaaaac aaccatgctt
    28621 gctacagggg gagcaggaaa tgtgtatcag aatacaacca atccgtcggt gtcgtcagga
    28681 gacggaattg caatggttta cagagccaag ggtattatcg acgatatgga gttttttcaa
    28741 tttcatccaa cagccctgta ccatccggtc gacaggccat cctttctcat taccgaagcc
    28801 ctcagggggc atggagcaat tctgaaaaat caaaagggaa ttccttttat gaagaaatac
    28861 gattctaggg aatcactagc tccgcgagat attgttgccc gcgcaattga tagtgaaatg
    28921 aaaatttctg gtcacgattt tgtgtattta gatgctacac aaattgatgc aaacgatttg
    28981 cagaataaat tccccaacat ttaccagaaa tgcaaatcgt acggaattga tattactcgg
    29041 gaatatattc ccgttacacc cgcagctcac tatttatgcg gaggaattgt tacagatatt
    29101 tacggtaagt cgtcaatcaa tttcctgtat gcagccggcg aaactgcctg taccggtttg
    29161 cacggagcaa acaggcttgc ttccaattcg ctgctcgaag ctgttgtttt tgctcacagg
    29221 gctgctgaac aagctatgtc cgatattgaa aaagcttgta ttaagacggc tatccctgat
    29281 tggaacgatg aaggtacaat tcacaatgaa gaaatggtac tgattactca aagcctgaaa
    29341 gaaatgagac agataatgag taattatgta ggtattgttc gcagtgattt gaggttgaaa
    29401 cgcgcttttg aacgattgga aattttgttt caagaaaccg aatcattgta taaatcctca
    29461 aaaatcaccc ctgaaatttg tgaactcagg aatatgataa atgtttcata tctgataatt
    29521 aaaatggcca tggctcggag aaaaagtatt ggactgcatt ataatattga ccatcaataa
    29581 attatgtaat tttgtataat tcatattgtg tattccatcg aaataataat tttgtttatc
    29641 tttgctcaca aacactatac atatgaaaaa aataatttta attcttgtaa caattgtact
    29701 ttatgcaaac agttttgcag acggtattaa acttaatgat gcccagtatg ctgcaaaaac
    29761 gtattattac caaaatgttc agtcgttcag agctcagggt tgggatgaaa ttaaccttca
    29821 actggtgaat aatcctgaag aaggtgttta cccaatgtat attttcaacg tgaatgaaaa
    29881 tgaaggattt atcattgtat cggctttaaa tacaatgaaa ccggttctgg catactcgtt
    29941 tgaaggagca tttaatcccg ggaacatgtc gccagggcaa acagcgttca ttcagtattt
    30001 tgccgacgaa acaatgttgg cactttcggg tgatatcgtt gtgaaggagg gagtttccgc
    30061 tgaatgggat cggttgctga ggtatacacc tggcaccagt ttcgaaaagg atgttactgt
    30121 gggcccactt gttaatgcca aatggaatca gaactggcct tacaatgcat catgccctgt
    30181 cgacgatgat tgccctgatt attcctccga caatcatgac cacgtttatg tgggttgtgt
    30241 tgccatagga atgcttcagg taatgaaata ctacaactgg ccaccatctg gtgaagggtc
    30301 aaaaacacac ctttcctgga caaatggcgg atatggtaac attactatta attttgcaca
    30361 acagacctat aactggtatg ccatgcctaa ttcgctggga ggaaccgaaa acagtgaggt
    30421 tgccaaaatc tgttatcatg caggggttgc tgtttcgatg tggtggggat gcgatggttc
    30481 agggtcgcaa accgacaaaa ttgtaactgc gttggaaaat tatttcaaat attcatccga
    30541 tgctcaattc attgaaaaaa gtgactatac tgattcaaat tggaaaacag ttcttagaaa
    30601 ccaattgaat cagaataagc ctatggtata tggaggaacg tcaactacgg tagggcatgc
    30661 atggacttgc gacggatatc agaataccga ctatttccat atgaactggg gttggggtgg
    30721 tgccggtgac ggattctatg ctcttgataa ccttgtgtca acagcaacac ctggggggcc
    30781 ggaaaacgac tttagtaccg gacaggaagc ggtgataaat atttatcccg aatcgggtta
    30841 tccattgtat tgtggtcaga atattaacct caatacccca agtggagcct ttggcgatgg
    30901 tagctcaaac cttgattacc agaacaatca acaatgctct tatttaattc agcctacctg
    30961 tggtgcagtt gttcaactga atttcgattc gtttgatctg gcagaaggcg acattgttag
    31021 agtattcaac ggtagtaatg ctgatgcaga ggttattgaa acatacacaa gcgaaaacac
    31081 tccgggaact gacaatattt atgctgaccg tggagccatg tacattcagt ttattaccga
    31141 tgggtctggc gtagcctccg gatgggatgc cagttatgtg gtgaaaaact gtaaaacaaa
    31201 tattgtgtat tacgaaccca gcggctcatt cgacgatggt agtggcgttt gcgattatgg
    31261 aagctcatca tcgacggttt gcacatggtt tattcaacct gaagatgctg cctggataaa
    31321 cattcacttc accgactttc acctcgccgg atctcccgac tatgtgaaag tattcaaaaa
    31381 ctcgaccggc tccgaaaacc ttgttgctca attttccagc actgcattac caaccaccga
    31441 cattatggta gaggcaggtg tggcagtagt tcaattcttt gccaacacta ccggcaacga
    31501 cgagggctgg gctttggagt atacttcatc gaccactgca gtagaaaacc tgagccttaa
    31561 acccgttgtg caggttttcc ctaatccttc tgaaggaaac gcgactataa atttttattc
    31621 acagggatca cacgaagcta cttttgttat cagcgacata accggtaaac tgattgccaa
    31681 taagcgaatc gcttcaagtg attgtatatt ccacacttca gttcaggatt tggttggtaa
    31741 tagcttaccc gatggtcttt atatccttga aatctattcc ggcagcaatt ctgttaccga
    31801 aaaagttatg attacacgtt aggattcgaa atattcgtat tcaaattctg ttacgatttc
    31861 gggggaatct tctttaagga gattccccca ttcgtttaca gtaattttgc tttcggaata
    31921 tgtatagcca tgagaattaa tatgcttttc tatccgagtt ttatcatcga tgtactctac
    31981 aagaataact gagccactca tcgatgtttg ctttacagga cggtttaatt catcatattc
    32041 caactttacc caattcaata tctggttttt ggcgtttgtg gtttttatca tttccagaaa
    32101 atcgttgtca ttatatatat attcctgaat ttgagtagtt ttatagttca agtcacgttc
    32161 ttctttccta acgggtcttt ttttattatc gaaagaactg aaaatatgcg tgatttcttt
    32221 gccatcttca ttgataacga cttcttttat gacatttccg taatcatcaa gctcctgaat
    32281 ctttgtttct tcaacctctc cttcctcatc ctgttccgaa atggtaattc tcttgcctga
    32341 atttgaatat tcatacaaaa acactgacaa gtaaccctgt ccgaaatcaa cggtttccct
    32401 aatgagctgt ccttcggaag aataatcata agttctgttc tccgacgatt cttgttcagt
    32461 atcgaaaaat gtgctgctta gcaaccggtg ttcgttatca aaaatatttt ccgattttga
    32521 aatgatctca ccgttcttat cgaaacgata ttcttcaact aaaaaaccat tttcatcaaa
    32581 cactttatac gagcttttat aacgaatatt atccgttttg atatcggtga atgctgtgat
    32641 tgttttgatt ttcattttgt aaatattaaa attcaaaatt attaaatgca aacaaaaaac
    32701 agtataattg taataaataa aacctaaaaa ctgaattgga aaagctattg tttattatta
    32761 accccaaagc ggggaatggt gatttggaaa aagtttgggg cgaggttgag caaacaataa
    32821 ggcggcatga tatcgagtat catcactatt ttactactgc acagaatgat ggttgtgagc
    32881 aggttaaact ttttctgaag cagggttaca gaaatattgt agtcttgggc ggcgatggta
    32941 ctatcaatga ggtcgtgaat ggaatttttc agcaacagga tgtgccatat gaagaagtgg
    33001 taatgggttt gatttcgatg ggaacaggga atgattgggg aagatattac aaatggacga
    33061 ccaatcacat tgacgcaatt catcagattc ttgacggaaa ttttataagg caggatatcg
    33121 gtgtcatcaa acattggata aacggacaac agcatacaag ttattttgca aatattgcag
    33181 gctttggttt tgatgctgct gttgtgaaag caacaaacat tatgcaggca aggggcaggc
    33241 ggaaaaaaag tgcatacctg cttaatctgc taaagtgcct gataaataat aagttgcttg
    33301 aaatgaaaat tgaatttgac gatcaaatta ttcacgacag catattttcg ataagtattg
    33361 gaaacgggaa gtactcagga gggggcatga tgcaaacgcc gagagcaatt aataacgacg
    33421 gacttcttga tattgcaatt taccggaata tcagaaagct aaaggttatt agtaatgtga
    33481 accgcttata taatggtact attgaaaaag tgagaggttt ggaaacttat cgcagcaagt
    33541 catttaaaat cagttgtcca cacacgactt ttgctgagat tgacggcgaa attgtaggag
    33601 atgggccata tgaaatcagc attcttccta atgctttaaa ggtatttgca aaaccttctc
    33661 taaagatata aagtttcccg cgagatgttg cgggtaaaaa atacttccca tataagtaac
    33721 ttaattgtgg atttttataa aaaattctat ccatatttcg ttactatttt aaatggtcat
    33781 acaacgaaat cctgtatata acgcacttta cttcagaagc catgagaaac gaaaaagaaa
    33841 acatattgct tgaagggtgt cgaaaccttg aaccaagagc tcaaaagcgg ctttatgatg
    33901 cctacaagaa ctgcatgttc actatttgta tgcgtataac aggtaattac gaagaggcca
    33961 gagatgcatt gcaggatggg tttatctcgg tttttaacga tatttctaaa ttcagaggag
    34021 agtccagcct gggggcttgg attaaaacca taataataag agcagcgctt aaaaggctga
    34081 aaccggtaag ttttattgca tcagacgaaa tacccgatgc tcctgtggaa tgggattctg
    34141 aattcaccgg agaggagctg caaaaagcaa tttgggcttt gcccgaaggt tacagggttg
    34201 tttttataat gtttgaggtt gaagggtact ctcataccga aatagccgat attttgcata
    34261 tcagtgaggg tacttcaaaa tcgcaattgc attacgcaaa aaagaaattg aaagaactac
    34321 ttaaaagtta taggtaatat ggaaactgat aatttgaaaa aagctatatc gcagttgcct
    34381 caatataagg ctcctgatgt ttggggaaaa ataaaaagcg aaatccctag aaaaagcccc
    34441 ggtaagttgc tgtttttttt cactgcagca ataattggtt gttccgtggt tgcaggactt
    34501 ttgctatcaa caccggaaac aattctatcg tcggaatgtt ttcaggcaag gacaccgaat
    34561 cgtccactaa tgcgatatgt aaaggatgtg gaagaaccag aaaccgctga agtcattctt
    34621 aaattcaata agtcgaataa aaaaactgaa atatcaacac ctgatacaac agccaagctc
    34681 caaaccatac cccaaaccag caatgaaatc gcatatgaaa caccacctga accgccgagt
    34741 ttctatcata taataaccag tgaaaaaatc aaatatgaag aaactgggag taatttaata
    34801 accaatggtg atttcgaaga atatgttttt tgtcctgcgg gttttaccga acgacctgtg
    34861 cgtaagctta ttccttattg ggaagttcca agtaaaggga ctcctgacta ctttaatgtt
    34921 tgtagcaaac gtgatgcagg tgtccccaaa aatttcgcag gcaggatttt tgcacattca
    34981 ggttctggtt atgctggact aattcttcgc agcagcttta caaacgagaa taaaataacg
    35041 ggcgtaaaac cgcttgacta ccgtgaatat attcaggctg aattaaatga gactttagtg
    35101 agaggaaaga aatacaagat tcaattttgg gtttgcaatt cctccggatc gcgctatgct
    35161 gttgatgctg tgggggcagt acttacccga gataaaatca aggctgaaaa tgacggagtg
    35221 ctcgactata cccctgttat aaagaactca agaaataata tgctggtaaa tcagtcatat
    35281 tgggttgcaa tagagggtgt ttacgaagct aaaggaggag agaagttcct gacaataggt
    35341 aattttaact ataactttgc aaccttgtat ataatgatgg acccgtcatc aaaatttaat
    35401 tatgcttact attacatcga cgatgttgtt gttgtagagg tgattgaaac tcttgaaaca
    35461 gaatatatca ttttggattc aacggtgaat gtggcccaga catgttcccc tcaatagttt
    35521 cacaattata aaggattgtt aattttattc agggcagtaa tccatttacg tttttcggta
    35581 ttaaacttag caatgttttc tggcttaaca ggttcagcag gaggggcttc gaagctcaac
    35641 gggtctattt tatatccatt ttcatgaatt tcataatgta ggtgaggccc cgtcgaccag
    35701 cctgtactgc caacataccc aattatatca ccctgtttta cgtagtcacc aacctgaaac
    35761 tgcccgaaac tgctgaaatg catatatact gtcgaatatg tgctgttatg aataacttta
    35821 acgtaatttc ctgcgccgct ttcataagct ctgatggcaa ttctcccgtc agatgcggca
    35881 aacaccggtg taccagcagg ggcagagtaa tctaccgccc tatgctcggt tactttatga
    35941 aaaataggat gcattctggc attcgtatag cggcttgaga cgcttgagta ctgaaggggt
    36001 gctttaagga aagacttctt catgctcata cccgaggtat cgaaaaactg caatatacta
    36061 tcctgaacaa atggaattgc ccataaatct ttgttgccgt gacgaaaaac agccgattcg
    36121 atttcgatgt tacctatact tttgccttca acagcaagtt ctgaatacaa cactttaaaa
    36181 taatcgcctt tttgcaatcc ataaaaatca attgtccatg caaaagtttg ggatagcttt
    36241 atcgccagct cccagcttaa tccctgatca acaagtgtat tccatagaga tgtctgaatt
//
LOCUS       DAOOQP010000015        36029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1365_length_36029_cov_6.311948, whole genome shotgun sequence.
ACCESSION   DAOOQP010000015 DAOOQP010000000
VERSION     DAOOQP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_36029_cov_6.311948"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLK75_02890"
     CDS             complement(<1..554)
                     /locus_tag="PLK75_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOY38221.1"
                     /translation="MYKFPTQKIREYASVRSVGASTPLCKAPLSNLYFSRNGDVISCC
                     FNRNFILGTYPTIPLRELWFGKQRTFFGKTVKKNELVRGCEMCIREIENQNYIGVIAK
                     QYDFIKPRRFYPYMLEFELDNVCNLECLMCEGQFSSSIRINREKEAKIITPYDSSFLE
                     YISPFLRKARLLRFGGGEPFLIPL"
     gene            995..2437
                     /locus_tag="PLK75_02895"
     CDS             995..2437
                     /locus_tag="PLK75_02895"
                     /inference="COORDINATES: protein motif:HMM:NF033640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitch domain-containing radical SAM protein"
                     /protein_id="HOY38222.1"
                     /translation="MKSHVLSYPKSDNGFSAISREKMVQYNKVRFYGARKHFCYNPFM
                     NLYFNASGYAIACCRSHLNVLGTYPEQSISEIWFGAKVNNLREHLLHNDLSMGCDYCR
                     AQIDANRFHSLPSLHAEKYASKTHVTYPDIIELELGNSCNLQCVMCSGRVSSSIRQHR
                     EKMEPLTSPYDSEFVKQLKEFIPHVKEMHFYGGEPFLISLYYPIWEAIIEINPKIQLR
                     AVSNGTVYNERIEKIIKATRFDLTISVDSLKKEHFGSIRVGADFDTVMENIRRFDKIC
                     SGRISISHTPMPLNYQETPEIIQFCNENDYTINLSYVEGPAEYALWSLHPSELDDIYR
                     YYCEVQWCKTKNRFIEHHNIMVFNEWKEQVKYFRDKNAAILQSFGDIEKEYELLMPQL
                     NQVFWDFFEQVPPGWLQFNQAMAMITETIDRTAITPVTIEGIRQLLTHFADSSVIQSE
                     IAGEFLRDEIRFRDYINDVFTPHRFWSRYY"
     gene            complement(2568..4022)
                     /locus_tag="PLK75_02900"
     CDS             complement(2568..4022)
                     /locus_tag="PLK75_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38223.1"
                     /translation="MRGTIGLTRKAAVRRMQWLKNRIAFSLRKGNHPSYAAALMDYQL
                     QRRTGPEKLLCHAPLRSVYFGFRGVVVPCCFNRETVYGTYPSNTVAEILNSNKRNKLQ
                     QILGNADFTAGCMHCLDLIESGNLEGVEANLYDGLKRNKGVNPGEMIFELSNTCNLEC
                     AMCEARFSSAIQHRQQGAVQEKPVYDKAFTEQLKPFLKNLEVAKFLGGEPFLINQYYD
                     IWEHIIATNRKCTINLQTNGTIYNQRIESLLKRGRFQIGVSVDSLIKDRFESIRKNAV
                     FEEVMANLDRFIAHTRSKGSFLNISVCPMQQNRDEIPQLVEFCNKKGVFVYFNTVYTK
                     SFNLRELDSTELYALKELYKSSPITGKGYVARRNKRSFGSLTQQIEEWYRRKLAEENR
                     CIKKYPYSKTDFIALFHQKILGGNQELYLKVDGLLQTLPDLIMLSDAQLEHLSNIPPI
                     EFTEVLLQCSDATLIALLNNFLEKDNFVYEKTSG"
     gene            complement(4101..5540)
                     /locus_tag="PLK75_02905"
     CDS             complement(4101..5540)
                     /locus_tag="PLK75_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38224.1"
                     /translation="MNLSRIYKSSPEVLQKLMISVTRLMNRMRYPMRIKSVPSGSSLP
                     AEQIHCYNKQRPYGPRPFLCYAPFTSMFFSRNGFMSPCYATYNHLSDKWPETSIKQAW
                     FNGQFEVLRSRMKLYDLDYACSFCKPLFLSGNFGSLLPCKYEPYGIGQQRYPKIMEFE
                     LSNRCNLECIMCDGNLSSSIRRNRDRLPAEPEHYNSAFVEELKEFIPHLLMAEFTGGD
                     PFLIPIYYDIWEQINTLNPHCQILITTNANTMSPRIEEMLRTYNNLHFNISFDSTKRE
                     HYEQIRVHADYDTVIQNIAIFAGYCHEHNTTCNLLVCPITLNSRDLYELVEFADSLNI
                     GIYFHTVVKPPELSLRTQSKEYLTDLIEYLGNFNPPRNTHLQQVNADNYQNLISLLVS
                     WRNANALQSNDVVQEWNTWLSSRFDKGIQEQIVAILGDFTNHESYIKLLIVLMEMEPD
                     DLRRILMSETDGMLRNRIVQFLDSGTGAG"
     gene            complement(5549..6793)
                     /locus_tag="PLK75_02910"
     CDS             complement(5549..6793)
                     /locus_tag="PLK75_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38225.1"
                     /translation="MHRFVYAVCGERYYIETLNFSLAFLRKFSNYPIWIITDSARNEI
                     PISHNNIVDIRTPVEYNNHQASIFLKTGLSIFLPKMEQGISCYLDSDVIVLGPECNNI
                     FHNYNPPVSFAPDHCAFGEYSPHAFQCSCEEEFVAGAETFRQKIKGYFPDFSFDSQQA
                     NADLKKLTALFGEMKKHPLRNTSTIIRYLIARYIFPVEKVQLGEFVFMKKNRCWYNAN
                     NEMISFDNRYHRKKLWKKHRIFFNNGDCYDENHRLLTPRTAHCTHLSEYLKKKYGVDI
                     PTSWRHWNGGVFLFDFRSEEFMKCWHNTVQHEFSNSLTKTRDQSALAMCVWKNRLEQH
                     PTLPVAFNFITGIGSGNIRWNADTGYSTPDANQIPDIHFLHVCNRWGDASWDIWQSVL
                     SLAAREGIEMNGDAGVPYSSKN"
     gene            6987..8363
                     /locus_tag="PLK75_02915"
     CDS             6987..8363
                     /locus_tag="PLK75_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38226.1"
                     /translation="MIYKNLRIDKGVLKKLNAHRNRSGHVCNAPFVAMSVGYDAQVSP
                     CCYTLQKDGVTGAAYYHTETLQEIWHGQVFANYRRMMKDRSLPPSCRLCREKLEAGDF
                     RGVKIREFDHLRESVVGPKLIELNIENTCNLECIMCNTVNSSGIARKHGIAARVPDAA
                     DVLRQLSAFLPVVEEMIFSGGEPFLSVTYQRIWEELIQINPDCRITVNTNATVINSKT
                     LALLEKGRFQFNVSLDSVQKVTYEKIRVNSRFETFFNNLELLLDYSRRKNVPLAAPVC
                     PLVLNYTELPEILDFCNCKGIYLNFLHVFGAYRYALQSAPPEILEKALHVFSNHRLPE
                     TGDVEKHNAGQFNSLVSDTRLHLSKSLKRKKKLTSVDKNSNSFQQEMVALSRHTYFDS
                     LQLHEAARIRNQIESAFALLPEAYQTGNVVNFFSESLHELPAELLYRLPACEIAEIIF
                     DIFDRSIN"
     gene            complement(8356..9564)
                     /locus_tag="PLK75_02920"
     CDS             complement(8356..9564)
                     /locus_tag="PLK75_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38227.1"
                     /translation="MNYFVYVVCGAKEYIDQLHFSLLFLRHFSKYPIYVATDTMRNEI
                     PIEHDNLIDVKTPQELNNHQAHLYIETSLHKHLHLYDGDKACYIDSDVIALNTEINDV
                     FSHFQAPVTFAGDHCTIDLFSPKITHCGCTEAYKQKAELYAEIQKILPCINPDDKETK
                     ADVLKHSELFAGLRNHPVKYFFKALTYCHQRYISRKPQFSFGGFSFVRKNAVWYNKRG
                     NIISFDFRNYNRLIHRKLGVRFNGKYWVDDKNNIIKPELPVCSHLREYLQKTYNIHIP
                     ENFRHWNGGVFLFGSVSQSFMDLWHEITMKESFAGNIMYYDDQSSLIATSFITGSQGM
                     GYLPDQFNFIADFNNPKICWNVEKGYSRDGFATAFKPAFIHVYHNWGDETWNIWQSVL
                     HLGRSEGILS"
     gene            complement(9604..10650)
                     /locus_tag="PLK75_02925"
     CDS             complement(9604..10650)
                     /locus_tag="PLK75_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38228.1"
                     /translation="MAIKAAVFNLGGHLVCEVLENGKWKMLDPDYGSYFLLNGSLAAV
                     SEISINKNECIPVFGQGLISEVTAIMPQNYISLFTSTDNNYIDKIYNTDIVWDKTSIS
                     LPPQSKFEIPIEINSDSCPFGNAVFACITVNEPFDGIIEIPFVIHKLNGSIQEFKPVS
                     DKNYELITEFFTPPGQYRLKCKDACIYMYINPILLESGASNTTISVISDNLASLNVSK
                     LKLTKRNKGSLWLNEYKVQFNSTLTEYKYDFLSAPENFDDTLNISSPEDFKNSIADFY
                     SKTHNGEIVPVSILDKTDKTLLLLKKYNFDTTKFLFTMQYPFLRNAMSILIIELPVDA
                     IEETFNFFPSKMWH"
     gene            complement(10601..11110)
                     /locus_tag="PLK75_02930"
     CDS             complement(10601..11110)
                     /locus_tag="PLK75_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38229.1"
                     /translation="MISVRRGSLSGFFTFITLLLITNCYSSQPAYGQQTEKNEQQTQT
                     VTSTAGEMASFSVSNISDSIVRFTFTVNDKLWFSPDEMLGYIRNLSCDTLPGTGQLIS
                     SAFSFVVNHTGHRCELPLANNFSYSPGYFVNSIGYGLCSNRAAVLSNILIKLGYKSRC
                     VQLGGAFGL"
     gene            complement(11265..12581)
                     /locus_tag="PLK75_02935"
     CDS             complement(11265..12581)
                     /locus_tag="PLK75_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38230.1"
                     /translation="MFTIGKKIQPSFSKEVLQEYFSATGCKHVVCNAPFSGLYFVPDG
                     TVKPCCALSPDAAFGNYNTTGIADLFKSDNRKKLQHLMKKNNLSFGCGNCASNIEKGN
                     YTGSISSLYYKFSPGKLPEVIDFELSHFCNLSCEMCYLHTASAVENTVYGDAFSEEIK
                     PFLKKLRTARFYGGEPFLIQSYYKIWEYIAQQNPRCHVHIQTNGTVLNEKILQVLGSC
                     DTFIGVSIDAAEKDTYENIRRGASFDTVLKNAAIFSEIMQKQGKSLTISICPMPQNFK
                     QIPEILKLADTLNAVVFFNTVNYPSRFNLGFITSDKLNYAATFLKDFRPGNSSNPNSL
                     SNWNKLQGIIQGIEYMAKKNSVTEKRLTPLKTDDFIRQLCERIQEPEVSEKVLLLMNK
                     ASVNTHVNIYLQDYIAGIEPLEIKHLINRIVKNSDVNYLQNIFNTE"
     gene            12793..13827
                     /locus_tag="PLK75_02940"
     CDS             12793..13827
                     /locus_tag="PLK75_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38231.1"
                     /translation="MLVTRIFRIQEWLVVKLLFNLAIAFAYILSTGISPADVLPISLL
                     VVLYIISVGSFGYYCNDMFDANGNLCRTYCYKRIRVKNIISVFVLITLVSLSFVPYLY
                     ALSEPNLYFILLAIHLVLLLSYPIPVLRIKSSVLGLIWDALYSYVFPATVTLSICSGY
                     HGVSLQFDIVTGFLVTAWLFFAGLRSILSHQLSDYDNDICTGTTTFTVRHGQTLSRRL
                     TTAATIAEWLLFVAVAVVVIDSLLIPIIASIIMFLVVEIGFRFRSFRRQTKINTAIAL
                     TNQLYDYYLFAGFFVWASVHWNLAAAVYPVTFLMIRLNAFSWCYHHIVLWVFHKLNGG
                     FKRLRKLFKS"
     gene            complement(13832..14623)
                     /locus_tag="PLK75_02945"
     CDS             complement(13832..14623)
                     /locus_tag="PLK75_02945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY38232.1"
                     /translation="MKQKAQTEKVSQYYDQWTHHYLSSGYGHIIQAHRPADVTELLEH
                     IAKNAGIRNGMRILDAGCGICGPAVYIAKHFDVNITAITISKVQADIAAEFVAENGLS
                     DRIEVIAGDYHSLDDLFPEASFDLILMLESFGHAVNPAQVLTCANTVLKKGGHIYIKD
                     YFAKEITGNTLRRKNMKRIIANMNKVYAYNLPDINQTIKTLRTLDLELQYIVRNKVPL
                     ANDDSVLDFEKKHGIDIFEGGFHYQILEPLELYFKKLIDVDAPIV"
     gene            complement(14675..15613)
                     /locus_tag="PLK75_02950"
     CDS             complement(14675..15613)
                     /locus_tag="PLK75_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38233.1"
                     /translation="MMHRSLLETYKLTDYPFRINWEITRVCNFSCPYCVNRQGESDAI
                     QYVFNPDEIAEFFNRTGQQWLILITGGEPFVYPRFAEVCRELTRNHYIQITSNLSHPD
                     VVFFSEQVDPGKVFLISASFHATERRSEKLRNEFIERCILLKNKGFNVLINYIAHPDT
                     FKQLDEDIQHFTNLGFGCFTLALRGRVNGLNYPESYTSEQWDIINKYALDTDIEKKAA
                     YGNLNFYGRYCQAGKYYFFMKPDGSVSRCATLQKSCGNLFEGSFTPGPEIRPCIAINC
                     YDVYCGLAALSGKKASLFAIHSEKKRYSKSSCLGCR"
     gene            complement(15617..16555)
                     /locus_tag="PLK75_02955"
     CDS             complement(15617..16555)
                     /locus_tag="PLK75_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38234.1"
                     /translation="MSKVRNIQEVLHKYEPGSKISLAELHFDGNDRMMTWQINNLCNF
                     NCPYCGHYVNDDPYVYKYSPEHISSCFDKDGFTWHIIITGGEPFLHKNIIPVCKLLTR
                     KHYISLNTNLSQANVYEFADTIDPSQVLVINASVHFSVRKERNIFDDYIKKFLYLQQK
                     GFNVVGSYVVYPASIDAFESDIEWLHAQGMKQVSSKVFEGEYNGKLYPDSYSEDELCK
                     ITKHMCCEIEMPQYLRYRRFKGLRCSTGRLMLSLKPNGDLERCLSEPTPLGNFFTGNY
                     ILPRHDKTCKTEICTCPYQGMLYTYRKSCGLPLFKK"
     gene            16710..17513
                     /locus_tag="PLK75_02960"
     CDS             16710..17513
                     /locus_tag="PLK75_02960"
                     /inference="COORDINATES: protein motif:HMM:NF018669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOY38235.1"
                     /translation="MIYFMHIIKTGGTTFDDILMRQYGVSKIVNLNNEEVDLEFNNLT
                     HSKKIKSELINKPDAIVVTGHFRIGLHNLMNDDKPRYIAFFRNPVEQYLSQYYFSISL
                     EEYPDIKEMTSSRGNIERFIYSDMLLYTENTQTFFLSEAPDRKTFQRDMEYLSPIAIQ
                     HVKDLFIFCAITDYFDESLVILRELLQWRKPIYYVKRKVTKNRPKAKTHNQKIIERIK
                     EINKLDLLLYDEALAVFNREKAKIRFFRWKLFKFKVQNFFFAYFHNKNL"
     gene            complement(17602..18747)
                     /locus_tag="PLK75_02965"
     CDS             complement(17602..18747)
                     /locus_tag="PLK75_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38236.1"
                     /translation="MFRYYIQVLGCAMRGVEAQRVNLYLQLNGGTETNLEEADIIVFF
                     SCSIISNTEEHTIESLKQFAPLNKRIILLGCSPGMSQVRIAEVFDGEMLATKDLEDID
                     RFFPEFEKKFSEIPLPSEYHFNFPYGEVYISDFLKFRTWGNRIRNMQPRLIVTSKGCN
                     NACSYCSVRKALGQLKSYPWSEIISQYKTSLNQGYKVFIFNGDDTGAFVTEDGKNFGD
                     LLLELHAVSAKKNIRWIIDNFHPQWFIKYFDIIKTLAEKKRIVEIILPLQSASDPMLK
                     MMRRKHTLAEVLPLIRELKATVPRVSVRTHFIIGFPGETIDDITNIKQLVALKYFSHI
                     VLLRYYETGNPSSQSFTPKIPAVVVEERILGLQAFIESHDIKCELTG"
     gene            complement(18797..20146)
                     /locus_tag="PLK75_02970"
     CDS             complement(18797..20146)
                     /locus_tag="PLK75_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38237.1"
                     /translation="MKKSLLLINPKTVNYPDFLFFPPISLGIIAAYTPDNWEVTIIDE
                     NTGALEFKHYDLVGITTSHHFLRAYQIAASFRNIGVTVVLGGLHSSAVPDEAGDHADA
                     IVSGDVEVVWKELLHDAESGELKRRYHGENTEMHFVKPITHFFRNNYFAYTLETSRGC
                     FNHCLFCGLHVPVHRPYRRKPVETIIAELSETPNPFVFIIDNNFYGRDEEYMFRLFDE
                     MDKNNIRKSFFAGVSIDFFKNEKLVKRASECGLRMVFVGFEADTEESLKALHKKNYSN
                     PSDIFAEYRNIVKLAHRNRILISGKIIIGMPDDTIEKIERRISFFKRLKLDEVSCAIL
                     TPLPMSRLFIQYRDSGDLLYNNFPQDWLKYDYNNLAFRHPKLDKTYVDRRQTEINMQQ
                     KPSMQSCLKALLITRSVKGTFLYYAYVHYFFNFTSRIIEKCFRLWGKSRNLHFPGSL"
     gene            complement(20143..21480)
                     /locus_tag="PLK75_02975"
     CDS             complement(20143..21480)
                     /locus_tag="PLK75_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY38238.1"
                     /translation="MMDENIAIYISGLSKRYPRVQKKFGISIGERIRDFFMKNKHFRE
                     NDYFYALHDINLSINKGESVGIIGRNGAGKSTLLKILAEVVEPTEGVIEINGTVASVL
                     EIGMGFHPELTGRENVYLSGTMLGIPRSIIAERFNEIVEFAGVHDFIDTPVKHFSSGM
                     YLRLAFSVVVNVDADILLFDEVLSVGDSEFHMKIFQKINELKQQGKTILLVSHNIHDI
                     GKFCERTVFLSKGCVANSGATGSVITDYYDDIFEFLYSEGEKKLLSKSRVVSNNISGL
                     NKGIENEYISVASPRVYAKGKSPDDDIFSSDDLVFELNFENKNDGQLVDIAWSLSQYD
                     NIFMIFTALNKGKNNLDKGKYFISGFLPANILNDSHFRVDVRIVVSCAAEPERVVFYA
                     NIPDAAAFRIHGDFPAHEKRFFGPLFLRTNWNFSVATESAEDKQNMSDQHMEK"
     gene            complement(21480..22298)
                     /locus_tag="PLK75_02980"
     CDS             complement(21480..22298)
                     /locus_tag="PLK75_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY38239.1"
                     /translation="MQTKIIRGGRGNSWKQLRLKKVWQYRSLIYAFTMRDIKSQYAQT
                     KLGIVWSLIQAITAALVINLFFGILIKMNTGSIPYILYAFPGMMSWYFFSYIVNQAGT
                     SLVQSQHIIKKVYFPKLILPLYKSLVGMVEHLIWLAVFFGMALLMAFPLQITLLALPI
                     CILTNIIAGLSVAVWLSALTVRYRDASHIIPFVIGFGVFVTPVFYKAAMIPEAYHFLL
                     YWNPMAGVIESYRWCLLGMPFDIHYIYGFIPMVVLLVTGLFYFRKVEGIIADVI"
     gene            22529..23356
                     /locus_tag="PLK75_02985"
     CDS             22529..23356
                     /locus_tag="PLK75_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY38240.1"
                     /translation="MLRKISILGGVGKDGNPEKVERLDLYMGDVISIVGPTGCGKTTL
                     INDIELFANKNTPSKRTILIDDLPISSEYSYDPSQHPIAMISQHTNFLSDLPVSEFLT
                     IHATIRGGSNIEELVDETIDFANQLTGEQIIRTSAMTELSGGQTRSLLIADAVIIGHS
                     PVILLDEIENAGIHRTKALSLLKNYKKIFVFVTHDPRIALLSDYRVVMKQGAMQRIIV
                     TNRDEKMAAVGLKKIDDVMLECRKLIRAGDEISDTVFSNLLTDMIEGCEEYLKTRPS"
     gene            23353..24054
                     /locus_tag="PLK75_02990"
     CDS             23353..24054
                     /locus_tag="PLK75_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOY38241.1"
                     /translation="MKLIIIAGPPTSGKTTVLRQVIRKQIEAGKRPSYLKIDVQFADE
                     DSKIKAEFSIPSRKVYSGELCPDHCNVVVMDQAIAWAQQQKTDFLFVETAGLCLRCSP
                     YVTNSLGVVVLEATSGMNLPRKIGPMLGLADVCVVTKIDLVSQAEREVFRYRIMQAAP
                     QVRIFETNALYGIGIDPIVKSIESSSEVAFPMILRGNPPVGTCTVCVGKKEIGAENHF
                     GVLRTLDNDLFYSGE"
     gene            24110..24769
                     /locus_tag="PLK75_02995"
     CDS             24110..24769
                     /locus_tag="PLK75_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOY38242.1"
                     /translation="MKESIFDLLPKLDCGACGFKNCADFADSVLSGLNTIDFCPYVDN
                     KKNTIQSTAIKPESLGQKLCWKDHLDRDFDFILDTFPGEPGPRETILPFNPLMVREMS
                     IVQGDILIGRPMGMSCGCPVTHCGTVMRSDPKSGVIDWCVTGPLDPRRQGYKDVGYYV
                     AHAYEGIVTDTKEIPVIGKRYWFMPRRCMLQWRHSGLVNSVARYANGGYRVRIEGLYI
                     G"
     gene            24800..25384
                     /gene="cysC"
                     /locus_tag="PLK75_03000"
     CDS             24800..25384
                     /gene="cysC"
                     /locus_tag="PLK75_03000"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOY38243.1"
                     /translation="MVLTNTREQKQSILKQKGHCIWLTGLPGSGKTTIAAALETLFLE
                     HDILVKVFDGDIIRSNINADLDFSEAGRMQNIVRTAHLNTELIACGLVVINAFVSPLN
                     LMRQKAIEIVGKPDFTLIHIDCPLEVCERRDPKGMYAQARQGKLKNFTGISDCYEAPV
                     NPDLVINTAAISVDDAVALIFNFVLPIIKPAHLL"
     gene            25381..26280
                     /gene="cysD"
                     /locus_tag="PLK75_03005"
     CDS             25381..26280
                     /gene="cysD"
                     /locus_tag="PLK75_03005"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOY38244.1"
                     /translation="MKHFSYIEQLESETIFIIREAVASFENPILLFSGGKDSLVMLHI
                     AMKAFYPANLPFPIMHIDTGHNFPETLKFRDDLVSKYNLRLIVASVQESIDNGRVIEE
                     TGINASRNYLQTITLLDAIEKHHFDVAFGGGRRDEEKARAKERFFSHRNEFGQWDPKN
                     QRPELWYLFNSKKHQGEHFRVFPLSNWTEKDIWQYICNKNIEVPTLYFSHLRECFYRD
                     GVLLAKSDYVPMKPDEKPKWMQVRFRTIGDMTCTGATLSDAATSEEVLHEIIAMRITE
                     RGGRFDDKRSETAMEDRKKEGYF"
     gene            26281..27543
                     /locus_tag="PLK75_03010"
     CDS             26281..27543
                     /locus_tag="PLK75_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOY38245.1"
                     /translation="MQQKNQYNKEAELLRFTTAGSVDDGKSTLIGRLLYDSGLIPDDQ
                     YDSIKLASERKGESEVNLALLTDGLRAEREQGITIDVAYRYFATKNRRFIIADTPGHI
                     QYTRNMVTGASTANLALVLVDARKGVTEQTRRHVFIASLLKIPHLVLCVNKMDLVDYE
                     KNVFDAIRSEFSEFSAKLEISDIQFIPVSALKGDNIVEKSEKMLWYEGPSLLYLLENV
                     HIGSDYNLIDCRFPIQSVIRPQNTDYRAYAGMVAAGIFKPGDKVMHLPSKLKTSIASI
                     EIGCESIAEAYPPMSVSMQLKDEIDISRGDMIVRENNSPTVSNQLEVMACWFSNKPLT
                     EKSQFILKHTTREAKVLVQEIRYKIDINTLHRIENSKQLELNEFARIRIRTSQALFYD
                     KYKLNRVTGSLIFIDEKTFETVGAGMIL"
     gene            complement(27548..29338)
                     /gene="asnB"
                     /locus_tag="PLK75_03015"
     CDS             complement(27548..29338)
                     /gene="asnB"
                     /locus_tag="PLK75_03015"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY38246.1"
                     /translation="MCGILGRWNFGKPIDKEVFNRMRDTLIHRGPDGFGTWIHDNGNL
                     ALGHRRLSFLDLSSAGAQPICNENKSIWLTVNGEIYNYLELREELRNKGHVFKSDTDS
                     EVIVHAWEEWGTEMLPRLEGMFAFGLWDENRKELILARDRYGIKPLYYCLNNNEMLFA
                     SELKAFAADPGIPRVIDPAAFCNYFTYRYIPSPQTIYKNIYKLEPGQCAVFKTQGDFS
                     LSFYFSPATDNVKMGINEAAEIAGELLHSSVRRHIRSDVPVGSFLSGGYDSSALVKYF
                     SKEQPGFNTFSAGFEGWDMSEHHFANKVSSVYGTRSHELIIGTNDYEVLPELMFNYDE
                     PIADISILPTYLISQLAAAHNKTVLSGEGADEIFAGYTWHRPYLWDITKKQLREARKY
                     HWEIPANRYDVESYSRAMAMGMFRHSDFKEFISPEWYQYIPEDTWWFYRKHYDNSLPA
                     PKSFQLLDIRTFMGELVLTKIDRASMAHSLEVRVPFLDNKLVDFMLSLDPDVYFSLKK
                     QKVLLYKLLKKDLPKQILQRKKQGFVGPDDYYKNIQFYTHYLGESMLSKAGVVNQNYI
                     NKLMSEGDYWRLWKICIMELWWRKWNPSID"
     gene            complement(29354..30298)
                     /locus_tag="PLK75_03020"
     CDS             complement(29354..30298)
                     /locus_tag="PLK75_03020"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY38247.1"
                     /translation="MRTVLITGGAGFIGSNLADKMLNKGFRVIAIDSFDDYYDLSVKK
                     RNIAQALKHPAYFFIENDINHPDGYISEIPANTECIFHLAARAGVRHSIQHPEGYQKA
                     NVDGTISIARVAEHLQIPKIVFTSSSSVYGNNPTRPWLEDSATNPMNPYASTKLKAEE
                     FLSDFSSQTGIEIVIMRLFSVYGRRMRPDLMMDKIFNCICSGKELTVYGDGSSERDYT
                     YIDDIINGLLLGMDYKGECYEVFNLGNNYPVQLSRIIHSFEKLMNQNAKINYLPAIGG
                     ESVSTCAGITKAVDLLAYNPSVNIETGIRLFIEWKHNN"
     gene            complement(30432..30740)
                     /locus_tag="PLK75_03025"
     CDS             complement(30432..30740)
                     /locus_tag="PLK75_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOY38248.1"
                     /translation="MEEYHVITTENGSYKTKLTAGFLKRQKYEPVDVNTIYALIPGTV
                     TEVLVKEGDTVAKSQQLLTVDAMKMNNIITSHTAGIVKKIHITKADKVAKGDVLIELQ
                     "
     gene            complement(30753..32297)
                     /locus_tag="PLK75_03030"
     CDS             complement(30753..32297)
                     /locus_tag="PLK75_03030"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOY38249.1"
                     /translation="MNNVEKNHELTLKQEKLQLGGGVQAIEKQKSMGKLTARERIDLL
                     LDKASFQEYDLFIKHEAHDFDMEKKELHGDGVVIGTGTVNGSPVCVFAQDFTVAGGSL
                     GLMHARKITKIMEVALTNRIPLIGINDSGGARIQEGVNSLAGYGEIFYLNTQASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDQVSKMFITGPEVIKTVLGEEISMEALGGARV
                     HAEITGNAHFFSKTETECFDTIRKLLSMIPANNSVKPAALQWQEPLNADKIAGIMPDD
                     PSMPYDVREVIKAVSDFSFFIEVQELWARNMVVGFGRIAGETVGFVANQPLYLAGVLD
                     VDSSDKAARFIRFCDAFNIPLVTLVDLPGYLPGVDQEHAGVIRHGAKVLYSYSEATVP
                     KITVILRKAYGGGYIAMSSRHLRADFVFAWTSAEIAVMGPQGAANIIFRKEISESADP
                     DATRNQKVDEYRERFANPYVAAGKGYVDSVIKPEETRRALINALSVCKGKSQIRVDKK
                     HGIPPF"
     gene            complement(32325..33404)
                     /locus_tag="PLK75_03035"
     CDS             complement(32325..33404)
                     /locus_tag="PLK75_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOY38250.1"
                     /translation="MKRIDFYIIKKFLGTFFFALALIIAISIVFDLSEKMDDFIDRNA
                     SLKTVVFDYYLNFIPYFANLFSGLFVFISVIFFTSKMAQNSEFIAMLSSGISFRRILI
                     PYFIAASIITAFSFVLGYYVIPSSNKVRLEFQETYMKGSGSHIGAIDMHRQISPGIYI
                     YIESYNITRNMGNKVTIEKFENNKLKSKLCADNMLYDSITNKWILQRYFIRNFDGIKE
                     SLIYGNRIDTTLRLSPKDFKKKKEIVEAMDHNELDAYIADQKLMGTPDVVNSEIEKYS
                     RISTPFSTFILTLIGVSLSSRKRKGGMGINIGVGILLSFSYVLFMKFANMFAINATFP
                     ASFAVWIPNILYALIALGLYFQAPK"
     gene            complement(33401..34531)
                     /gene="tgt"
                     /locus_tag="PLK75_03040"
     CDS             complement(33401..34531)
                     /gene="tgt"
                     /locus_tag="PLK75_03040"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOY38251.1"
                     /translation="MNFSIRKYDISTKARCGMLETAHGSIETPVFMPVGTAGTVKGVH
                     ARELRDDIHAQIILGNTYHLYLRPGIDIIHESGGLHKFSGWQHPILTDSGGYQVFSLA
                     ANRKLTEDGAIFKSHIDGSKHIFTPENVVEIQRKIGADIIMAFDECTPFPCEYEAARK
                     SMLLTHQWLERGWKHFCNTEPLYGYVQSFFPIIQGSVYKNLRQQSADAISSYDANGYA
                     IGGLSVGEPNEVMYEMIETVNEVLPSGKPRYLMGVGTPANILEAVALGVDMFDCVMPT
                     RNGRNGMLFTMNGTINIKNKKWENDFSCIDEDGDVFVDKDYSKAYLHHLIISNEILGA
                     QIASIHNLGFYIKLMRTIREKIHTGEFLSWKNRVVNTIMKRR"
     gene            complement(34727..35182)
                     /locus_tag="PLK75_03045"
     CDS             complement(34727..35182)
                     /locus_tag="PLK75_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38252.1"
                     /translation="MKINSNIFLTFFAILPLFFLVSNGFAQQHQSRYDFTFPDGTILS
                     SETGTIRAELKLSSGPYDEFIVGIYSANKSSENATTKSNPVVSGGVALVRFNNESSNI
                     KKGDPVTSSSEPGVAMKATEPGIILGVALEDAGESGDLLKIRVMIQYMK"
     gene            complement(35200..>36029)
                     /locus_tag="PLK75_03050"
     CDS             complement(35200..>36029)
                     /locus_tag="PLK75_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38253.1"
                     /translation="GAAGATGPAGAAGATGPAGAAGATGPAGAAGATGATGPVGCTNA
                     NYVIKSNGTSGTCSQIYDNGTNVGIGTASPTQKLDVAGAVKFSGALMPNNQAGTSGQI
                     LVSQGAGVPPVWQNPATVPMYGNNAHSVKLTTIQGTTSTTFTDIPGMSLTVTTVHNTF
                     YVFASFTARVANSSTHNAAMGQIVVSAQIVVDGVAQAKAASVITDYDFDDFYGEVTVT
                     SGGVAFAGIPVTLAPGSHTIKIQWSIAGYFWESGAWVEINPITFTNSGDHCILTIFD"
BASE COUNT        10411 a         7312 c         7347 g        10959 t
ORIGIN      
        1 tacaatggta tgaggaatgg ttcaccacca ccaaaacgca acaaccgggc ttttcgcaga
       61 aacggtgaaa tatattccaa aaaactgctg tcgtaggggg ttataatttt cgcttccttt
      121 tctctgttga tgcgtatgct actgctaaac tgaccttcac acatcaagca ttcgaggttg
      181 catacattat caagctcaaa ctcaagcatg tacggataga agcgtcgagg ttttataaag
      241 tcgtactgct tggcaataac accgatgtag ttttggtttt cgatttcacg gatacacatc
      301 tcgcaccccc tgacaagctc attttttttc actgtttttc caaaaaatgt tcgctgtttt
      361 ccaaaccaca attcacgaag cgggatagtc gggtaggtac caagtataaa atttctgttg
      421 aaacagcaac tgataacgtc tccgttacgg ctgaagtaca aattcgacag tggagctttg
      481 cacaaggggg ttgaagcccc gacactgcga actgatgcat actcccgtat tttctgcgta
      541 gggaacttgt acataatttt aataaatctg cattttagcg ttcattaaat actgcgaatt
      601 cattctgtaa ttatatattt cacttataaa catgcagtat ggttactgca aatatacgtt
      661 gtttgaaaat aatttgtttt ttttattcta tatagctaaa aaaacaaaca ttaagcttac
      721 aagttttaaa acacataaaa tatgaaaacg acaaaaacgc aacaatgaag aagctcttaa
      781 aacatgcaat aattgagtct gctgattttt tattagtcct gagtaaatgc ctgtttcctg
      841 aattcttatt tatccggtgt ttgtaaaaaa taagtacatt tgatgcaaat tgacgaaggg
      901 aacacctcca ttttgtaatt gagacaaata aatgagtcgc caaaataaaa gattattaaa
      961 ccttgtatat ccgctgtggg gtaaatataa cagtttgaaa tcacacgtgc tttcgtaccc
     1021 taaatcggat aatgggtttt ctgctatttc gcgcgaaaaa atggttcagt acaacaaagt
     1081 gcgtttttat ggagccagaa aacacttttg ttacaatcca ttcatgaatt tgtattttaa
     1141 tgcctcaggc tatgctatag cttgttgccg atcacatttg aatgtattgg gaacataccc
     1201 tgagcaaagc atttccgaaa tatggtttgg agccaaagta aacaatctca gggaacatct
     1261 gttgcataac gatttatcga tgggatgcga ttattgcagg gcacagattg atgccaaccg
     1321 ctttcattcc ttaccatcgc ttcatgctga aaagtatgca agcaaaacac atgtcactta
     1381 tcccgatatt attgaacttg aattggggaa ttcgtgtaat ttgcagtgtg ttatgtgctc
     1441 aggaagggtt tcgtcttcaa tacgacaaca ccgcgaaaag atggaaccgc ttacatcgcc
     1501 ttacgacagt gaatttgtca aacagcttaa agagttcatt ccgcatgtca aggaaatgca
     1561 tttttacggt ggagaaccat tcttaatttc gttatattat ccgatatggg aagcaattat
     1621 cgaaataaac cccaaaatac agctgcgggc tgtcagcaat ggcactgtgt ataacgagag
     1681 aattgaaaaa attattaaag caacccgctt cgaccttaca atttccgttg attcactgaa
     1741 aaaggaacac tttgggagca ttcgtgttgg agctgatttc gatacggtaa tggagaatat
     1801 cagaagattt gataaaatct gttctggccg catttctata agtcataccc ctatgccgct
     1861 taattatcag gaaacacctg aaattattca gttttgtaac gaaaatgatt acacgataaa
     1921 cttgtcatac gttgaaggac ctgcagaata tgcattgtgg tcgcttcacc cttcggagct
     1981 ggatgatatt taccgatatt attgcgaagt gcaatggtgc aaaacaaaga accgattcat
     2041 cgaacaccac aacatcatgg tgtttaacga atggaaggaa caggtaaaat atttcagaga
     2101 taagaatgct gcaatattgc aatctttcgg ggatatagaa aaggagtatg agttacttat
     2161 gccccaactt aatcaagtat tttgggattt ttttgaacag gttccacccg gatggttgca
     2221 attcaatcag gcaatggcga tgattacgga aacaatagat cgcacagcta taactccggt
     2281 tacaattgag ggtatcaggc aattgcttac tcactttgcc gatagcagcg taatacaatc
     2341 cgaaatagca ggagagtttt tacgtgatga aatcagattc cgcgattata ttaacgatgt
     2401 ttttactccg caccgttttt ggtcgcgtta ttactgagaa tattctacac actgttattg
     2461 atagcccgtt gggaacagat tgctttgtcg ctcgggtcag ctccgcaatg acgggcactt
     2521 tcagattctt acacaagaac tttaataaaa gggatacgcc aagggagtca accgcttgtt
     2581 ttttcataca cgaaattatc tttttccaag aaattattga gtaaagctat gagagttgcg
     2641 tcggaacatt gcaacaaaac ttcggtaaac tctatggggg gaatattgga taaatgttcg
     2701 agttgggcat cgctgagcat aattaagtcg ggtagtgttt ggagtaagcc atccactttt
     2761 aagtataact cctgattacc acctaaaatt ttctgatgaa agagtgcaat aaaatcagtt
     2821 ttactgtacg ggtatttttt aatacagcga ttttcttcag cgagttttct ccggtaccat
     2881 tcttctatct gttgggtcag cgaaccaaag cttcttttgt tgcgtcgggc aacatatcct
     2941 tttccggtaa taggagagct tttgtaaagc tcttttaatg catacagttc ggtagaatcc
     3001 agttctctga ggttgaacga tttggtgtac acagtattaa aatacacaaa tactcccttc
     3061 ttgttgcaga attcaaccag ttgaggaatt tcgtcgcggt tttgctgcat cggacaaacc
     3121 gaaatgttca gaaaactgcc tttactgcgg gtgtgggcaa taaaccggtc gagatttgcc
     3181 ataacttctt cgaaaacggc atttttgcgt atcgactcaa agcgatcttt tataagtgaa
     3241 tcaacggaga ctcctatctg aaaccttccc cgtttcagca gactttcgat tctttggttg
     3301 taaatggttc cgttggtttg aaggttgata gtacattttc ggtttgtggc gataatatgc
     3361 tcccagatgt cgtagtactg attaattaaa aagggttctc cgccaagaaa tttggctact
     3421 tcgaggtttt taagaaaagg cttgagttgt tcggtgaaag ccttgtcata taccggtttt
     3481 tcctgtactg cgccttgttg ccggtgttgt attgccgatg aaaaccgggc ttcgcacatt
     3541 gcgcattcaa ggttgcaggt attgctgagc tcgaatatca tttcgccggg gttaacccct
     3601 ttgtttcgtt tcaggccgtc gtataaattt gcttcaacgc cttcgaggtt gcccgattca
     3661 atgaggtcaa ggcagtgcat gcagccagcc gtgaaatcag cgttgccgag tatttgctgt
     3721 aatttgttgc gtttgttgct gttcaatatt tcggctacag tgtttgaagg ataggtacca
     3781 tacactgttt cgcggttgaa acaacacggc actaccactc cccgaaaacc gaaatataca
     3841 cttcgcaatg gtgcatggca caacaatttt tcggggccgg tacgtcgttg taactgataa
     3901 tccattaatg ccgccgcata cgaaggatga ttgccttttc ggagcgaaaa agcaattcgg
     3961 ttcttcagcc attgcattct gcgtacagca gctttccgtg ttaaaccaat cgttccccgc
     4021 atcccgattc ttttaaataa ttacaaatat acacattcgt tgctatctct gtatcggtaa
     4081 aacttcagag aagtcgtttg tcaacctgca cctgtcccgg aatcgagaaa ttgaacgatt
     4141 ctgtttcgca gcataccgtc tgtttcactc atcaaaattc tccggagatc atcaggctcc
     4201 atttccatta atacaatcaa tagtttgata taactttcat ggttggtaaa atcaccaagt
     4261 attgcaacaa tttgctcctg tatgcccttg tcgaaacgac tcgatagcca ggtattccat
     4321 tcctgaacca catcgttgct ttgtagtgca ttggcattgc gccacgaaac caagagggat
     4381 ataagattct ggtaattgtc ggcgtttacc tgttgtaagt gggtattccg cgggggatta
     4441 aagtttccga gatattcaat caaatcggtc agatactcct tcgattgggt tctcaacgac
     4501 aattcaggcg gttttacaac ggtgtgaaaa tagatgccaa tattaagcga atcggcaaac
     4561 tcaaccaatt catacaaatc gcggctgttc agggttatcg ggcaaacaag cagattgcag
     4621 gtggtgttat gctcatggca gtaccctgcg aagatagcta tgttttgtat tacagtatca
     4681 taatcggcat gcacccttat ttgctcgtaa tgctcacgtt tagtactgtc gaacgatata
     4741 ttgaagtgaa gattgttgta ggttctgagc atttcttcaa tccgcggcga catggtattg
     4801 gcattggttg taattagtat ctggcaatgg ggattcaggg tgttgatttg ttcccagata
     4861 tcgtagtata tgggtataag gaaagggtcg ccaccggtaa attccgccat gagcagatgg
     4921 ggtataaact ctttaagttc ctcgacaaaa gccgaattat aatgctccgg ctctgcaggc
     4981 agccggtctc tgttcctgcg aatggatgag ctcagattgc cgtcgcacat aatgcattcc
     5041 agattgcaac gattgctcag ttcaaactcc ataattttag ggtatcgttg ttgtccaatg
     5101 ccatagggtt cgtacttgca gggcagcaga ctcccgaaat ttcccgataa aaacaagggc
     5161 ttgcaaaagc tgcatgcata atcgagatca tacagtttca tgcgggatcg caacacttca
     5221 aactgaccgt tgaaccatgc ctgcttaata gatgtttccg gccatttgtc ggagaggtgg
     5281 ttgtaagtgg catagcatgg cgacataaat ccgttgcgac taaaaaacat acttgtaaaa
     5341 ggtgcgtagc ataaaaaggg tcggggacca tagggccgct gtttgttgta gcagtgtatc
     5401 tgttcggcag gcaacgaaga tccggatgga acagatttta tacgcattgg gtagcgcatt
     5461 ctgttcatca ggcgtgtaac agaaatcatg agtttttgta aaacttcggg agatgattta
     5521 tatatgcggc tcagattcat tgctcaaatc aatttttgct gctgtatggc actcctgcat
     5581 cgccgttcat ttcaatgcct tcgcgtgctg ctaacgataa caccgattgc cagatatccc
     5641 acgaggcatc gccccagcgg ttacatacat gcagaaaatg aatgtcggga atttgatttg
     5701 catcgggtgt tgaataccct gtgtcagcat tccaccttat gttaccggag cctatgcctg
     5761 taataaaatt aaaggctaca ggtaaggtcg ggtgttgttc aaggcggttt ttccatacac
     5821 acatggccag tgccgattga tcgcgtgtct tcgtaaggct gttgctaaac tcatgctgaa
     5881 cggtattgtg ccagcatttc ataaattctt ccgaccggaa gtcgaacagg aaaaccccac
     5941 cgttccagtg cctccagctt gttggaatgt caacaccgta tttttttttc aggtactccg
     6001 acaggtgtgt gcaatgagca gtgcgtggag tgagtaaacg atgattttcg tcgtaacaat
     6061 cgccattatt gaaaaatatt cggtgttttt tccaaagttt tttacgatga taacggttgt
     6121 cgaaacttat catttcgttg ttggcgttgt accagcaccg attttttttc ataaacacaa
     6181 attcgcccaa ttgtactttc tcaacaggga atatataacg tgcaatcaag tagcggataa
     6241 ttgtggatgt attgcgtaat ggatgctttt tcatctcacc gaacaacgca gtgagttttt
     6301 tcaagtctgc attggcttgc tgcgaatcga agctgaaatc gggaaaataa ccttttattt
     6361 tttgtctgaa agtttcagct ccggctacga attcttcctc gcaactgcat tggaatgcat
     6421 gtggactata ctccccaaat gcgcagtggt caggtgcaaa gcttaccgga ggattgtaat
     6481 tatgaaaaat gttgttgcat tcaggcccca gtacaattac atcggaatcg agatagcaac
     6541 tgataccttg ttccattttc ggaagaaaga ttgataagcc ggtttttaga aagatgcttg
     6601 cctgatggtt gttatattca accggagttc ggatatcaac aatgttgttg tgtgaaatgg
     6661 gtatttcatt gcgggcagag tctgtaataa tccagatggg gtagttgctg aacttcctta
     6721 gaaacgcaag gctgaaattc agtgtttcga tgtagtacct ttctccgcaa acggcatata
     6781 caaacctgtg cataaactct tatataaaac accttttaca aatgtagcaa ttgttttaaa
     6841 gaaccgtgcg ctgcattgcg ttttttagcg acacattcaa tacagttatt aaacaaataa
     6901 tgtttcggtg cctgagataa tggtgtatga aatatttcgc tacatttata atggcgaact
     6961 gaagaattta agcatattgt aactgcatga tatataaaaa cttacgcatt gataagggtg
     7021 tcttaaagaa acttaatgcc caccggaacc ggagtggtca tgtgtgcaat gcgccctttg
     7081 ttgccatgtc ggttggttac gatgcacagg taagtccttg ttgttatacc ttgcagaagg
     7141 atggggttac cggagcggca tattaccata ccgagacgtt acaggaaatt tggcatggac
     7201 aggtattcgc caattaccgg cgtatgatga aggatcgcag tctgccacca tcgtgccgct
     7261 tatgtcgcga aaaacttgaa gccggcgact tcagaggtgt gaagatcagg gaattcgatc
     7321 atctgcgtga atcggttgtt ggtccgaaac tcatagaact gaatatcgaa aacacctgta
     7381 acctcgaatg cattatgtgc aacacggtga actcctccgg tattgcccgc aaacatggca
     7441 ttgcagccag ggttcctgat gcagcggatg ttctcaggca attgtcggct tttttgcctg
     7501 ttgttgagga aatgatattt tcgggtgggg agccatttct gtcggtaaca taccagcgca
     7561 tctgggaaga attgatccaa ataaacccgg attgcaggat tacggtgaat accaacgcca
     7621 ctgtgattaa cagtaaaacg ctggcattgc tcgaaaaagg acgattccag tttaatgttt
     7681 cgctcgacag tgttcaaaaa gtaacctacg aaaaaatcag ggtgaactcg cggttcgaaa
     7741 cctttttcaa caatcttgaa ttgcttctcg attattcccg ccggaaaaat gttcctctgg
     7801 ctgccccggt ttgtccgttg gtgttaaact acactgagtt acctgaaatt ttagatttct
     7861 gcaactgcaa aggcatttac ctcaatttcc tgcatgtgtt cggtgcctat aggtatgcat
     7921 tgcagtcggc tccaccggaa attctcgaaa aggcacttca tgttttttca aaccaccgtt
     7981 taccggaaac gggtgatgtg gaaaaacaca atgctgggca attcaacagc cttgtttctg
     8041 atacaaggct gcacctgtcg aaatcgctga agaggaaaaa aaagcttact tcagttgata
     8101 agaacagtaa tagttttcag caggagatgg tagcactttc aaggcatact tatttcgatt
     8161 cgttgcagtt acacgaagcg gctaggattc ggaatcaaat agagtccgca tttgcactgc
     8221 tgccagaagc ttatcaaaca gggaatgttg tgaatttttt ttctgaaagc cttcatgaac
     8281 tgccggcaga gcttctgtat cggctgcccg catgcgaaat cgctgaaata attttcgata
     8341 tcttcgaccg gagtatcaac taagaatgcc ctcactacgg cccagatgca gcaccgattg
     8401 ccagatattc caggtttcgt cgccccagtt atgatatacg tgaataaagg ctggcttaaa
     8461 agctgttgca aaaccgtcgc ggctatatcc cttttctaca ttccagcata ttttggggtt
     8521 attgaaatcg gcgataaaat tgaactggtc gggtaaatac cccatccctt gactaccggt
     8581 aatgaatgat gtagcaataa ggctgctctg atcgtcgtag tacataatat tacctgcaaa
     8641 cgattctttc atggtgattt cgtgccataa atccataaac gattgcgaaa cagagccgaa
     8701 gagaaataca cccccgttcc agtggcggaa attttcaggg atatgtatgt tgtaggtttt
     8761 ctgaagatat tccctgaggt gactgcatac aggaagttcc ggtttaataa tattattttt
     8821 atcgtctacc cagtattttc cgttaaatct aaccccgagc tttctatgta ttaaacggtt
     8881 ataattcctg aaatcgaagc tgataatatt tccccgcttg ttataccaca ctgcgttttt
     8941 tctcacaaaa ctgaagccac caaaactgaa ttgtggtttt cgcgagatgt agcgctggtg
     9001 gcaataagtc agggctttga aaaagtattt caccgggtgg tttctcaaac ccgcaaacag
     9061 ttcggaatgc ttcaacacgt ctgcttttgt ttccttatca tcagggttta tacagggcaa
     9121 tatcttttga atttcggcgt ataattctgc tttctgtttg tatgcctcag tgcagccgca
     9181 gtgcgttatt ttgggactaa ataaatcgat ggtgcaatgg tcgcccgcga aggttacagg
     9241 agcctggaaa tggctaaata catcatttat ttcggtgttc aatgcaataa catcgctgtc
     9301 gatatagcac gctttgtcac catcgtataa atgtaaatgt ttgtgtaaac ttgtttcgat
     9361 gtacagatga gcctgatgat tgttcagctc ttgaggagtt ttaacatcaa ttaaattatc
     9421 atgttctatt ggaatttcat ttcgcattgt gtcggttgca acatagatgg ggtacttcga
     9481 aaaatgcctg agaaatagta atgaaaaatg taattgatcg atgtattcct ttgccccgca
     9541 aacaacataa acaaaatagt tcataccaat tggttcttgc aatgcaagat acaaacattt
     9601 agcttaatgc cacattttcg aggggaaaaa attgaaggtt tcctcgatag cgtcaacagg
     9661 caactcaatt atcaatatgc tcatggcatt tctcagaaag ggatattgca ttgtgaacaa
     9721 gaatttggtc gtatcgaaat tatacttctt caataatagc aatgttttat ctgttttatc
     9781 cagaattgaa acgggaacaa tttcgccatt atgggttttt gaataaaagt cagctatact
     9841 atttttaaaa tcttcaggac tggatatgtt taaagtatca tcgaaatttt ccggagccga
     9901 aaggaaatcg tatttgtatt cagtaagggt gctattaaat tgtactttat attcgttgag
     9961 ccataaacta cctttatttc gttttgttaa ttttaatttg cttacattga gtgaagctaa
    10021 gttgtcgctt attacagata ttgttgtatt tgatgcacct gattctaaca gaattgggtt
    10081 gatatacata taaatacatg catctttgca ctttaacctg tactgtccgg gtggtgtgaa
    10141 aaattcagtg atgagttcgt aattcttatc agagactggt ttgaattctt gaatactccc
    10201 attcaatttg tgaattacaa atggaatttc gatgattcca tcaaaaggtt cattgactgt
    10261 aatacaggca aatactgcat taccgaatgg acagctgtcg ctatttattt caatgggaat
    10321 ttcaaatttt gactgcggtg gcaacgaaat agaggttttg tcccaaacaa tatccgtatt
    10381 gtaaatttta tcaatatagt tgttatcggt tgaagtgaac agcgaaatat agttttgtgg
    10441 cataatggca gtgacttcgc taattaaccc ttgcccaaac accgggatgc attcattttt
    10501 attgatactg atttcactaa cagcagccag gctaccattt agcaaaaagt acgacccgta
    10561 atccggatca agcattttcc atttcccatt ttcaagaact tcacaaacca aatgcccccc
    10621 caagttgaac acagcggctt ttatagccaa gcttaattaa aatgttcgac agcacagcag
    10681 ctctattgct gcataaacca tatccgattg aattaacaaa gtatccagga gaatatgaaa
    10741 agttgtttgc tagtggtaat tcacatctat ggccggtgtg gttaaccaca aaactaaagg
    10801 cagacgaaat caactgtcct gttccgggca acgtgtcgca gctcaaattc cgtatgtagc
    10861 caagcatttc atcagggctg aaccacaact tgtcgttaac cgtaaaagtg aaacgaacaa
    10921 tggaatcact gatgttgctg acgctgaaac ttgccatttc acccgcagta ctagtcaccg
    10981 tttgggtttg ttgttcattc ttctcagttt gctgtccata agcgggttga gaggaataac
    11041 aattagtaat tagcaaaagc gttataaacg taaaaaagcc ggataggcta ccccttcgca
    11101 ctgaaatcat aaaaaatgga atataaatgg taaaaataaa acagtaattg taaataatag
    11161 gaacttcggt ttgaaaccgg aataattcgc tgatatgcga acaatatttt gggcaatatt
    11221 tccccctctg taatgcaaac ttatgttgca gcttttgttg tggctcattc agtgttgaaa
    11281 atgttttgca gataattcac atcgctgttc ttaacaattc tgttgattaa atgtttaatt
    11341 tctaaaggtt caatacctgc aatataatcc tgcaggtaga tatttacatg tgtattcact
    11401 gaagccttat tcataagaag caatactttt tcggatactt ctggctcttg aatcctttcg
    11461 catagttgtc ttataaaatc atcggttttc aatggcgtga gtcttttttc ggtaacggaa
    11521 ttcttttttg ccatatactc aataccttgt ataattcctt gcaacttgtt ccaattgctt
    11581 aggctattgg ggttgcttga gttaccgggt cgaaaatctt tcagaaatgt tgctgcatag
    11641 ttaagtttgt cagatgtaat aaaccccaga ttgaagcgtg atgggtaatt tacagtatta
    11701 aagaaaacca cggcattaag cgtatcagca agcttcaaaa tttcgggtat ttgcttgaag
    11761 ttttgcggca ttgggcaaat tgagatcgta agcgattttc cctgtttttg cataatttcg
    11821 ctgaaaatag cagcattctt tagtacggta tcgaagctgg ctcctctgcg gatgttttcg
    11881 taggtgtctt tttctgcagc atcaatgctt actcctataa aagtgtcgca gctgccaagt
    11941 acctgtagaa ttttctcgtt aagtacagtt ccgttggtct gaatgtgtac atggcaacgc
    12001 ggattctgct gtgcaatata ttcccaaatc ttatagtatg attgtatcaa aaatggttca
    12061 cccccataaa aacgtgcagt tcgcagtttt ttcagaaaag gtttgatttc ttcggaaaat
    12121 gcatcaccat acactgtgtt ttcaaccgca gaagcagtat ggagatagca catttcacaa
    12181 ctcaggttgc agaaatgtga gagctcaaag tcaatgacct cgggtaattt tcctggacta
    12241 aatttataat acagcgacga aatgctacct gtgtaatttc ccttttcgat atttgatgca
    12301 cagttaccac aaccaaacga caggttattt tttttcatca aatgttgcag ttttttcctg
    12361 ttatccgatt taaacaggtc ggcaataccg gtagtattat aattcccgaa tgccgcatca
    12421 ggcgataaag cgcaacaagg tttaactgtc ccgtcaggaa cgaaatacaa tccgctgaaa
    12481 ggagcattac aaactacatg tttacaacct gtggcagaaa agtattcctg taatacttcc
    12541 ttactgaagg agggctgtat ttttttacca attgtaaaca ttacctactg attctctttt
    12601 caaaaatacg caaaacaacc ttgcttttaa ttaaatgttg gtactgaata gcattcttat
    12661 acaaaagagc taatatttta aaccggatgt ttaaatatat atatttatta ttaacgtttt
    12721 ttatttttga tactaattcg atatagtaag aattacataa gatttgctaa ctttacttta
    12781 aaatcttcag agatgttggt aacccggatt tttcgaattc aggaatggtt ggttgtgaaa
    12841 ttgttattca atttagctat tgcatttgcg tatatcttaa gtacaggtat tagcccggca
    12901 gatgttttac ctatatcctt acttgtagta ctttatatta tatcggtagg ctcgttcggg
    12961 tattattgca acgatatgtt cgacgctaac ggaaatttgt gccgtaccta ctgctataag
    13021 cgtataaggg taaaaaatat tatttccgta ttcgttttga ttactttggt ttctctttcc
    13081 tttgtcccct atttatatgc cctgtccgaa ccaaatttat attttatatt gcttgccata
    13141 catttggtac tcctgctcag ctatccgata cctgttttgc ggataaaaag ttcagtattg
    13201 ggtcttatct gggatgcttt atattcctat gtgtttcctg ctacggttac cctgagtatt
    13261 tgctcgggat atcatggtgt gtcattgcaa tttgatattg ttaccggttt tctcgttact
    13321 gcatggttgt tttttgcagg cttacgcagt atactaagcc atcagttgtc ggactacgat
    13381 aacgatatat gcacgggcac aacaactttc acagtgaggc acggtcagac tcttagccgc
    13441 aggttaacta cagcagccac catcgcagag tggttacttt ttgtggcagt ggctgttgta
    13501 gttattgatt cactcctgat tccaataatc gcatcaatca tcatgttttt ggttgtagaa
    13561 attggttttc gatttcggag cttccgccgt cagacgaaaa taaatactgc aatagcgctg
    13621 acaaatcagt tgtatgatta ttatttattt gccggctttt ttgtttgggc atctgtacat
    13681 tggaaccttg cagctgcagt ttaccccgtt acttttctta tgatccgtct gaatgcgttt
    13741 tcttggtgtt atcaccatat cgtgttatgg gttttccata agttgaatgg aggtttcaaa
    13801 cgtttacgta aattgtttaa aagttaagcc tctaaactat tggggcatcg acatcaatca
    13861 gttttttaaa atacaattcc agcggttcaa gtatttgata atgaaaacca ccctcgaaaa
    13921 tgtcgatacc atgttttttt tcgaaatcca gcaccgaatc atcgttggca aggggtactt
    13981 tattacgcac aatatattgt agttctaaat caagagtacg cagtgtttta atggtttggt
    14041 taatatccgg aagattgtag gcataaacct tgttcatgtt ggcaataatg cgtttcatgt
    14101 tttttctccg cagggtgttc cctgtgattt ccttggcaaa gtaatcttta atataaatgt
    14161 gacctccttt tttcaatact gtgtttgcac aggtaagaac ctgagcgggg ttcactgcgt
    14221 gaccaaacga ttcaagcata agtatcaaat cgaagcttgc ttccgggaac aaatcgtcta
    14281 aagaatgata atcgccggca ataacttcga ttctatcact cagtccgttt tcagcaacaa
    14341 attcagctgc aatatctgct tgcactttcg agatagttat ggcagtgata tttacgtcga
    14401 agtgttttgc aatataaact gcaggtccgc atattccgca acctgcatct aaaattctca
    14461 tcccgtttct tatacctgca tttttagcaa tgtgttccaa taattcggtt acatcggcag
    14521 ggcggtgtgc ctgaatgata tgtccataac ccgaactcag gtagtggtgt gtccattggt
    14581 cgtagtactg tgacactttt tcggtttggg ctttttgttt cattgcaggg taactagttt
    14641 aagtttatat ttcggctaaa gtattcattg ctgtttacct gcagcctaag cagctggatt
    14701 tagagtatct ctttttttcg gaatgtattg caaataacga tgcttttttc ccgcttaggg
    14761 cagccagacc acagtagaca tcgtagcaat ttattgcaat acatgggcgt atttccggtc
    14821 ccggtgtaaa actgccttca aataggtttc cgcaactttt ctggagcgtg gcgcatcttg
    14881 aaacactgcc atcaggtttc atgaaaaaat agtatttacc cgcctggcaa tatcgaccat
    14941 aaaagttcag attcccatag gcagcttttt tttcaatatc ggtatcaagt gcatatttgt
    15001 ttattatgtc ccattgttcg gaagtgtacg attcgggata attcagccca ttcacgcggc
    15061 ctctcaatgc cagggtaaaa caaccaaacc cgaggttggt gaaatgctga atatcttcat
    15121 caagctgttt gaatgtatca gggtgcgcaa tgtaattaat taatacattg aatcctttgt
    15181 ttttcaggag aatacagcgt tcgataaatt cgttccgaag tttttcggat ctgcgttcag
    15241 tggcatgaaa cgaagcgctt ataagaaata ctttccctgg gtcaacttgc tctgaaaaaa
    15301 aaacaacatc agggtggctc agattgcttg tgatttgaat gtaatgattg cgcgtaagct
    15361 ccctgcatac ttcagcaaac cgcggataca caaaaggttc tcctccggtg atgagtatca
    15421 gccattgttg accggttcga ttgaaaaact ccgctatttc atccggattg aaaacgtact
    15481 gaattgcatc gctttcgccc tgccgattta cacaatacgg gcaactgaaa ttgcatacac
    15541 gggttatttc ccagttgata cggaatgggt agtcggttaa cttgtatgtt tcgagcaatg
    15601 acctatgcat catggtttat tttttaaata acgggagacc gcaacttttc ctataagtgt
    15661 aaagcatccc ctgatatgga catgtgcata tttcggtttt acaagtttta tcgtgtcggg
    15721 gtaaaatgta attgccagta aaaaaattcc ctaatggtgt cggctccgac aaacatcgct
    15781 caaggtcgcc attgggtttt aacgatagca ttaatctgcc tgtactgcag cgcaagcctt
    15841 taaacctccg atacctgagg tattgaggca tttcaatttc gcagcacata tgttttgtta
    15901 ttttacataa ttcatcctcc gaataagaat caggatatag ctttccgtta tattcgcctt
    15961 cgaacacttt cgatgatacc tgtttcattc cttgtgcatg caaccattca atatccgatt
    16021 caaaagcatc gatagaagcc ggatatacca catacgaacc aactacgtta aaccctttct
    16081 gctgcagata cagaaatttc ttaatataat catcaaaaat gttccgttct tttcgaaccg
    16141 aaaaatgaac acttgcgtta atgacgagaa cctgtgaagg atcaattgta tcggcaaact
    16201 cgtaaacgtt tgcctgcgac aggttggtgt ttagcgaaat ataatgtttc cgggttaaca
    16261 gtttacatac aggaattata ttcttgtgca aaaaaggctc tcctccggta ataattatat
    16321 gccatgtgaa accatccttg tcaaaacatg aggagatatg ttcgggagaa tacttatata
    16381 cgtaagggtc atcgttcaca taatgaccgc agtaggggca attgaaattg cagaggttat
    16441 ttatttgcca ggtcatcata cggtcgttcc cgtcgaaatg caattctgcc agacttattt
    16501 tgctgccggg ctcatattta tgcaacactt cctgaatatt tcggacttta ctcattctcg
    16561 ctgttttcag ttctgtaaaa gtaataattt atagcacacg ggtaaaatag aatttaatga
    16621 atataaccct taaaaaacac gtatcgaatt gactcatttt ttttaccttt gactaatgct
    16681 ggtattgata atttaatgat acgagggaaa tgatttattt catgcatatt ataaaaaccg
    16741 gaggtactac ttttgatgat attttaatga gacagtatgg ggtttcaaaa attgtaaacc
    16801 ttaataacga agaagttgac cttgaattta ataatttgac tcattccaaa aaaattaaat
    16861 cggaattgat aaataaacct gatgcaattg ttgtgacagg tcattttaga ataggattac
    16921 ataatttaat gaatgacgat aaacctcgtt atattgcatt ttttcgcaac ccagttgaac
    16981 aatatctgtc tcaatattac tttagtattt ctttagaaga gtacccagat ataaaggaaa
    17041 tgacatccag tagaggtaat attgaaaggt ttatttattc tgatatgctt ttgtacacgg
    17101 aaaatacgca aacttttttc ctcagtgaag ctcctgacag aaaaaccttc caaagagata
    17161 tggaatattt gtcacctata gctatacagc atgtgaaaga cctgtttata ttttgtgcta
    17221 tcaccgatta ttttgatgaa tcattagtaa ttttacgaga gttattacaa tggagaaagc
    17281 caatttatta tgttaagcgt aaagtgacta aaaacagacc taaagctaaa actcataatc
    17341 agaaaattat tgagcgaata aaagagatta ataagttaga cttattgctt tatgatgagg
    17401 cattagctgt ttttaatcgt gaaaaagcca agataagatt tttcaggtgg aaattattta
    17461 agtttaaagt gcaaaacttt ttcttcgctt atttccataa caagaattta tagtaaatag
    17521 ggaatctttc taatatcttg aactaaattc tagtctcaaa taataaaatt actcaggtat
    17581 ttttattaat ataaaaagaa tctatcctgt gagttcacac tttatgtcat gactttcgat
    17641 aaaggcttgt aaccccaaaa ttcgttcttc aactactacg gcaggtattt tgggagtgaa
    17701 gctctgagac gaaggattgc ctgtttcgta ataccgtaac agaacaatat ggctgaaata
    17761 cttcaaggca accagttgct tgatatttgt aatatcgtct atggtttctc cgggaaaccc
    17821 gataataaaa tgtgttctca ccgacactct gggaactgtt gccttcaact cccgtatcag
    17881 cggtaatacc tccgcaagag tgtgcttgcg gcgcatcatt ttgagcatgg ggtcggaagc
    17941 cgattgcagc ggtagtatta tttcgacaat tcgttttttt tccgcaagtg ttttgataat
    18001 atcaaaatac ttgataaacc actggggatg aaaattatct attatccacc ggatattctt
    18061 ttttgcagat actgcatgta attcaagaag caggtctcca aaatttttac cgtcttctgt
    18121 tacgaaggca cctgtatcgt caccattaaa aatgaatact ttatagccct gattaagcga
    18181 agttttatac tgggaaataa tttcgctcca tgggtaactt tttaattgcc ccagtgcttt
    18241 tcgcaccgaa caataactgc atgcattgtt acatccttta ctagtaacaa tcaggcgtgg
    18301 ttgcatatta cggattcggt tgccccaggt gcgaaacttg agaaagtcgg agatgtagac
    18361 ctccccatac ggaaaattga agtggtactc gctgggtaaa ggtatttcag aaaacttttt
    18421 ttcaaattca gggaaaaacc tgtcgatatc ttctaagtct tttgtggcaa gcatttctcc
    18481 atcgaagact tctgcaatgc gcacctggct cattccgggg gaacacccaa gcaatataat
    18541 tcttttattc aatggagcaa attgcttcag gctttcgata gtatgctctt cggtgttcga
    18601 tattattgag cacgaaaaaa acactataat atcagcctct tcaaggtttg tttcagttcc
    18661 accgtttaat tgtaagtaca ggtttacccg ctgcgcttca accccccgca tggcacaacc
    18721 aagaacctgt atataatatc ggaacatagg caattgttat aaataataaa cagtcataat
    18781 acatataatt aggtgtttac aggcttccgg ggaagtgaag atttcgcgat tttccccata
    18841 atctaaaaca cttctcaatt attctgcttg tgaaattaaa aaaataatgt acatacgcat
    18901 aatacaggaa tgttccttta accgaacgag taatcaacaa ggcttttaag cagctctgca
    18961 ttgaaggttt ttgctgcata ttaatttcgg tttgccttct gtcaacataa gttttgtcga
    19021 gttttgggtg cctgaatgcc agattattgt aatcgtattt cagccagtcc tgcggaaagt
    19081 tattatatag taagtcacca ctgtcgcggt actgaataaa caagcgcgac ataggcagcg
    19141 gggtaaggat agcacacgac acttcgtcaa gtttaagtcg cttgaagaag gatattcttc
    19201 gctcaatttt ttcgatggta tcatcaggca tcccaataat gattttccct gaaatgagta
    19261 ttctgtttcg atgggcaagc ttcacaatat ttcggtattc tgcaaatatg tcggacggat
    19321 tgctatagtt ttttttatgg agtgctttta acgattcttc ggtgtcggct tcgaaaccaa
    19381 caaataccat ccttaaacca cattccgatg cacgcttaac caatttttcg tttttaaaaa
    19441 aatcaataga taccccggca aaaaacgatt tccggatatt gtttttatcc atttcatcaa
    19501 ataagcgaaa catatactcc tcgtcccgtc cgtagaagtt gttatcgata atgaacacaa
    19561 aagggtttgg tgtttcgctg agttcagcaa ttattgtttc aacgggttta cggcgatatg
    19621 gcctgtgaac cggtacatgc aacccacaaa acaggcagtg gttgaagcac ccacgggatg
    19681 tttcaagtgt gtatgcaaag taattgttcc taaaaaaatg ggttattggt tttacaaaat
    19741 gcatctctgt gttttcgccg tggtaccttc gcttcagctc accggattca gcatcgtgaa
    19801 gcagttcttt ccataccact tcaacatctc cggaaacaat tgcgtcggca tgatcccctg
    19861 cttcatcggg taccgcagaa gaatgaagtc cccccagaac aactgtgacc ccaatattac
    19921 gaaaagaagc tgcaatctga tatgctctta aaaaatgatg ggaagtggta attccaacaa
    19981 gatcgtagtg cttaaattca agcgcaccgg tgttttcatc gattatcgtt acttcccaat
    20041 tgtcaggagt gtaggccgct ataattccca atgaaattgg cgggaaaaaa agaaaatccg
    20101 gataatttac cgttttagga tttattaata gaagcgattt tttcatttct ccatgtgttg
    20161 gtcactcatg ttttgcttgt cttctgctga ttcggttgca acgctgaaat tccaattagt
    20221 acggaggaac aaaggcccga aaaacctttt ttcgtgtgcc ggaaaatcgc catgaatcct
    20281 gaaagccgct gcatcaggaa tatttgcata aaataccacc cgttcaggtt cggcagcgca
    20341 actaaccact attcgcacat caaccctgaa gtggctgtcg ttcaaaatat tagcaggtaa
    20401 aaacccggaa ataaagtatt tccctttgtc cagattgttt ttacccttgt ttaatgcagt
    20461 gaatatcatg aaaatattat cgtattgact taacgaccat gctatatcaa ccaattgacc
    20521 atcgtttttg ttttcaaaat ttaattcaaa aaccaaatca tcagaactga atatatcgtc
    20581 atcgggtgat ttgcccttgg catatacacg tggtgaagct actgagatat actcattttc
    20641 aataccttta ttaagcccac ttatattatt gcttacgacc ctggactttg acaatagttt
    20701 tttctcacct tcggagtaaa gaaattcgaa aatgtcgtcg taataatcag taattacgct
    20761 tcctgttgct cctgagttgg ctacacaacc tttgcttaga aaaacagtcc gctcacagaa
    20821 ctttcctata tcgtgtatgt tgtggcttac aagtaatatg gttttccctt gttgcttgag
    20881 ttcatttatt ttctgaaaga ttttcatatg aaattccgaa tcgccaacac tcaacacttc
    20941 gtcgaaaagc aggatgtcgg catcaacatt aactactacc gaaaatgcaa gacggaggta
    21001 catgccactg gaaaaatgct ttaccggggt atcgataaaa tcgtgcactc cggcaaattc
    21061 tacaatttcg ttgaagcgtt cggctataat actgcgggga atacccagca ttgtaccgct
    21121 taaatacaca ttttcgcgtc ctgtaagttc gggatgaaaa cccattccaa tttcgagtac
    21181 tgaggccact gttccgttta tctctattac accttcggta ggctctacta cttcggcgag
    21241 tattttgagc agtgtactct tcccggcccc gttcctgcct attataccca ccgattcacc
    21301 tttgtttata cttaggttta tatcgtgaag tgcataaaaa taatcgtttt cacggaagtg
    21361 cttatttttc ataaaaaaat cccgtatgcg ttctccaata ctgataccga attttttctg
    21421 aacccgcggg taacgcttgc tcaagccgga aatatatatg gcaatattct cgtccatcat
    21481 caaattacat cagcaattat accttccact ttacgaaaat aaaataggcc tgttaccagc
    21541 aataccacca ttggaataaa tccataaatg tagtgaatat cgaaaggcat tccgagcaag
    21601 caccatcggt acgattcaat tacccctgcc atgggattcc agtataaaag gaaatgatat
    21661 gcttccggaa tcatggctgc tttataaaat acaggggtaa caaacacccc gaaacctatc
    21721 acaaacggaa taatatgcga ggcatcgcgg taacgtacag ttaaggcact taaccatacc
    21781 gccacgctaa gtcctgcaat aatattcgtt agaatgcaaa ttggtaatgc aagcagtgtt
    21841 atctgcaagg ggaaagccat aagcaatgcc attccaaaga aaacggcaag ccatatcagg
    21901 tgttccacca taccaaccaa tgatttatac aagggtaaaa tcagtttcgg aaaatacact
    21961 tttttaataa tatgttgtga ttgcacaagc gacgtaccgg cctgattgac aatatacgaa
    22021 aaaaaatacc agctcatcat ccccgggaaa gcatataaga tgtagggaat gctacctgta
    22081 ttcattttta ttaaaatacc aaaaaacaaa tttattacaa gcgcagcagt gatagcttga
    22141 attaaactcc acacgattcc caatttggtt tgggcatatt gcgacttaat gtcgcgcatt
    22201 gtaaatgcat aaataagcga acggtattgc cagacttttt tcaagcgcaa ctgcttccac
    22261 gagttcccgc gaccacctct aattattttt gtctgcaagt aatcaatgtg ttaatcatta
    22321 caaaaatact atttttttat gagatttgcc ctagtgtttc tattgtggca ggccttaaag
    22381 gttctatgat tgaaaaacag ctttggttta cattatttga agttattgta attaaaaaaa
    22441 tggagggttt tgtttaacct gatactgacc ttttaataga tttacaaata agcttttatc
    22501 tttgtaatga attcaaatgg gttttgcgat gctgagaaaa atttcgatat tgggtggagt
    22561 gggtaaagat ggtaaccccg aaaaagtgga aaggctcgat ttgtatatgg gtgatgtgat
    22621 tagtattgtt ggtccgaccg gttgtggtaa aacaacactc atcaacgata tcgaattgtt
    22681 tgccaataag aatacacctt caaaacgtac tattctgatt gatgacttgc caatcagcag
    22741 tgaatattct tacgatcctt cgcaacaccc cattgccatg atttcgcaac acacaaattt
    22801 tctgagcgat ttgccggtca gtgaatttct caccatacat gctactatac gaggaggttc
    22861 aaatattgaa gaattggtgg atgaaacaat tgattttgca aatcaactaa caggagaaca
    22921 gataataagg acttcggcaa tgacagaact atcgggtggg caaacgcgtt cattgcttat
    22981 tgccgatgcg gtaatcatcg ggcattcccc ggtaattctg ctcgatgaaa tcgaaaacgc
    23041 cgggattcac cgcacaaaag ccttaagcct actgaaaaac tataagaaaa tatttgtttt
    23101 tgttacccac gacccacgca ttgcattact ttccgattac agggtggtaa tgaaacaagg
    23161 cgcaatgcag cggattattg taaccaaccg cgacgaaaaa atggcggctg taggattgaa
    23221 aaaaatagat gatgtaatgc tcgaatgccg aaagcttatc agagcaggcg atgaaatatc
    23281 agacacggtt ttttcgaatc tgcttaccga tatgattgaa gggtgtgaag aatacttaaa
    23341 gaccagaccg tcatgaaact tattataatt gcaggaccgc ccaccagtgg aaaaacaacc
    23401 gttctgcgac aagtaatccg caaacaaatt gaagcaggga aaaggccatc ctatttaaaa
    23461 atagatgttc aatttgctga cgaagactca aaaattaaag cagagttttc gattccttcc
    23521 agaaaagtat attccggaga gctttgtcct gaccattgca atgtagtggt tatggatcag
    23581 gctattgcat gggcacaaca acaaaaaacc gattttttat ttgttgaaac tgccggttta
    23641 tgtctgcgtt gttctccgta tgttaccaat tcacttggtg tagtagtgct cgaagcaacc
    23701 agcggaatga atcttcctcg taaaatcggg ccaatgttgg gtttagcaga tgtatgtgta
    23761 gttacaaaaa tcgaccttgt ttcgcaggct gagcgtgaag tatttcggta tcggataatg
    23821 caggctgctc cacaggtaag aatatttgag accaatgcgc tttatgggat tggtatcgac
    23881 cctattgtga aaagcattga gagttccagt gaagtagcat tccctatgat actcaggggg
    23941 aaccctcctg taggcacatg cactgtttgt gtcgggaaaa aggaaatcgg agcagagaac
    24001 cattttgggg tactccgcac actcgacaat gatttgtttt attcggggga gtaaaacata
    24061 gttgtaatac tccaaaaaat aattatattt ttgcatttaa acctgactaa tgaaagaaag
    24121 tatatttgac ttactgccga aacttgactg tggtgcctgc gggtttaaaa attgtgctga
    24181 ttttgctgat tcagttttat caggcttaaa cacgattgac ttttgtccgt atgtggataa
    24241 taaaaaaaac acgattcaat ctacagctat aaaaccggaa agtttagggc aaaagctgtg
    24301 ttggaaagac catttagatc gcgattttga ttttattctt gacacttttc ccggagaacc
    24361 gggccctcgt gaaacgatac tgccctttaa cccactcatg gtgcgcgaaa tgagtattgt
    24421 ccagggagac atcctcatcg ggcgcccgat gggaatgtcg tgcggatgcc cggttacaca
    24481 ttgcggaacg gttatgaggt cagatcctaa gtcaggggtt atcgattggt gtgtaaccgg
    24541 tccgcttgat ccaagacgac aaggttataa ggatgttggc tattatgtgg ctcatgctta
    24601 cgagggtatt gttaccgaca caaaggagat tcccgtaatt gggaaacgtt actggtttat
    24661 gcctcgcaga tgtatgttgc aatggcggca cagtggactt gtaaattctg tagcccggta
    24721 tgcaaacggt ggatatcggg tgcgaatcga aggcttgtat atcggataga atgatgaatg
    24781 atttgaatat tagcgaataa tggttttaac aaacacacgc gaacaaaaac aaagtattct
    24841 aaaacagaag gggcattgta tttggctaac ggggttgccg ggctccggta aaaccaccat
    24901 tgccgcagcc ttggaaacgc tttttttgga acatgacatt cttgtaaaag tttttgatgg
    24961 cgacattatt cgctcgaata ttaatgctga tctcgatttt tcggaagcag gcagaatgca
    25021 aaacattgtc aggacagctc acttaaatac tgaacttatt gcatgtggtc tggtagtgat
    25081 taatgctttt gtcagtcctt tgaatttgat gcgacaaaaa gccatcgaaa tcgtgggcaa
    25141 gcccgatttt acactgattc atatcgactg ccctttagaa gtatgcgaaa ggagagaccc
    25201 taaaggtatg tatgctcagg caaggcaggg taaactcaaa aattttactg gaattagcga
    25261 ttgctatgaa gcccccgtta atcctgattt agtcattaat actgctgcaa tcagtgttga
    25321 cgatgcggtt gcattaattt ttaattttgt attaccaatt attaaaccag cgcacttgct
    25381 ttgaagcatt tttcatatat agaacaacta gaatcggaga cgatctttat aattcgcgaa
    25441 gcagttgctt cgttcgaaaa cccgatatta cttttttcgg gagggaaaga ttcactggtt
    25501 atgctacata ttgcgatgaa agcattttac ccggcaaatc tccctttccc cattatgcat
    25561 atcgacacag ggcataattt ccccgaaacc ttgaaatttc gcgatgacct ggtttcgaag
    25621 tacaatctca ggcttattgt tgcttcggtg caggaatcaa tcgacaatgg gagggtaata
    25681 gaagagacag gaataaatgc aagcagaaat tatctgcaaa caattacctt acttgatgcg
    25741 atagagaaac atcatttcga tgttgccttc ggcggaggcc gacgggatga agaaaaagca
    25801 agagctaagg aaaggttttt ttcgcatcgc aacgaatttg gtcaatggga tccaaaaaac
    25861 cagagacctg aattatggta tctgtttaat agtaaaaaac atcagggaga gcatttcagg
    25921 gtttttccac tgagtaactg gacagaaaaa gacatttggc aatatatctg caacaaaaat
    25981 attgaagttc ccacattgta tttttcacac ctccgtgaat gtttttaccg cgatggcgta
    26041 ttgctggcca aatcggacta tgttcctatg aagcctgatg aaaaacctaa atggatgcaa
    26101 gtaaggtttc gcactattgg agatatgacg tgtaccggag caaccctttc agatgctgcc
    26161 acatcggaag aggtgctgca tgaaatcatt gcgatgcgta taacagaaag aggcggacgg
    26221 tttgacgata aacgatcgga aacggcgatg gaagaccgca aaaaggaggg gtacttttaa
    26281 atgcaacaga aaaaccaata taataaagag gctgaattgc tgcgttttac taccgccggc
    26341 agtgttgatg atggaaaaag tacgcttatc gggaggctcc tttacgactc cggtttgata
    26401 cctgacgatc aatacgatag tataaagtta gccagtgaaa gaaaaggaga aagcgaagta
    26461 aacctcgctt tactaaccga tggactgcgt gcagaaagag aacagggaat cactattgat
    26521 gtggcgtatc gttattttgc aaccaaaaac cggcgcttta tcattgccga cacccccggg
    26581 cacatccagt acaccaggaa tatggtaacc ggggcatcta ctgctaacct tgctctggtt
    26641 cttgtagatg cacgaaaggg tgtcacagag caaacccgcc ggcatgtatt cattgcttca
    26701 ttactgaaaa ttcctcactt ggttttgtgt gtaaacaaaa tggatctcgt tgattacgaa
    26761 aaaaatgttt tcgatgcaat acgctcggaa ttctcagaat tctcggcaaa gctcgagatc
    26821 agtgacattc agtttattcc agtttcagca ctaaagggcg ataacattgt tgaaaaatcg
    26881 gagaagatgc tctggtatga aggcccatct ctcctgtatc tgctcgaaaa tgtgcatata
    26941 ggcagcgact ataacctgat tgattgtaga tttccgatac agagtgttat ccgtccacaa
    27001 aacaccgatt acagagctta tgctgggatg gttgcggccg gtatattcaa acctggtgac
    27061 aaagtaatgc atctgccttc gaaacttaaa accagtattg catcaatcga aattggctgt
    27121 gaatcgatag ccgaggcata ccctcccatg tcggtttcga tgcagctgaa agatgaaatt
    27181 gacataagcc gcggcgacat gattgtgcgc gaaaacaatt ctccaactgt ttcgaaccaa
    27241 ctagaagtta tggcgtgctg gttttctaat aaacctctta ccgaaaaaag ccagtttatt
    27301 cttaaacata ctacacgcga agcaaaagtc ttagttcagg aaatacgata taaaattgat
    27361 attaatacgc tgcatcgtat cgaaaactcc aaacaactgg aattgaacga atttgccaga
    27421 attagaatca gaacttctca agctttgttt tacgataaat acaaattgaa tcgtgtaact
    27481 ggcagcctga tttttattga tgaaaaaact tttgagacgg tgggagcggg aatgattctg
    27541 tagcgaatca atcaatgctt ggattccact tccgccacca caattccatg atgcaaattt
    27601 tccacaagcg ccagtaatcg ccttccgaca ttagcttgtt gatatagttc tgatttacga
    27661 cgccggcttt tgacaacata ctttcgccaa gataatgcgt gtagaactga atgtttttgt
    27721 aataatcgtc cggccctaca aagccttgtt ttttcctttg caatatttgc tttggcaaat
    27781 ctttcttcaa tagtttataa agcagaactt tctgcttttt caaactaaaa tatacatccg
    27841 ggtcgaggct gagcataaaa tcgaccagct tattgtcgag aaaaggaacc ctcacttcga
    27901 gggaatgtgc catgctagcc ctgtcgattt tggttagcac cagttcaccc ataaatgtac
    27961 gaatgtcgag caactgaaaa gattttggag ccggcagcga gttgtcgtaa tgttttcgat
    28021 agaaccacca cgtgtcttca ggtatgtact gataccattc aggcgagata aattctttga
    28081 aatcagaatg acgaaacatc cccatcgcca ttgctcttga ataactttcg acatcataac
    28141 ggttagccgg tatttcccag tgatattttc gggcttcgcg aagctgcttt ttcgttatat
    28201 cccacaaata gggtctgtgc catgtgtatc cggcaaaaat ttcatcagcg ccttcacccg
    28261 aaagcactgt tttattgtgt gcagccgcca attggctaat cagataggta ggaagaatgc
    28321 tgatgtcggc aataggttcg tcatagttga acatcaactc cggcaacact tcataatcgt
    28381 tagttccaat gataagttca tgcgaccgtg ttccatacac cgacgacacc ttatttgcaa
    28441 aatgatgttc actcatatcc caaccctcaa atccggcaga aaatgtatta aacccgggtt
    28501 gctctttcga aaagtacttc actaatgccg aggaatcgta cccaccgctt aaaaacgacc
    28561 cgaccggaac atcactgcga atatgcctgc gcacagagct atgaagcaat tcacctgcaa
    28621 tttctgctgc ttcattgatt cccattttta cgttgtcggt tgcaggcgaa aaataaaagc
    28681 tcagcgaaaa atcgccttgg gttttaaaga ccgcacactg accgggttcc aacttataaa
    28741 tattcttata aatagtttgt ggcgatggaa tgtagcgata ggtgaaatag ttgcaaaagg
    28801 cagctgggtc aataacacgg ggaataccgg gatcggctgc aaaagccttg agctccgaag
    28861 cgaacagcat ttcgttgtta ttcaggcaat aatatagcgg ttttatacca tatcggtcgc
    28921 gggcaagaat gagttccttt cggttttcgt cccaaagtcc gaaagcaaac attccctcaa
    28981 gtcgtggtaa catttcggta ccccattctt cccaggcgtg aacaataact tcgctgtcgg
    29041 tatccgactt aaaaacatga cctttattac ggagttcttc gcgcaactca agataattgt
    29101 aaatctcgcc attcacagta agccaaatac ttttgttttc gttgcatatg ggctgagctc
    29161 cggcagaaga aaggtcgaga aatgacaagc gcctatgacc aagagcaagg tttccattgt
    29221 cgtgaatcca tgtgccaaaa ccgtccgggc cacgatgaat cagcgtatcg cgcatacggt
    29281 tgaaaacctc tttatcgatt ggttttccga agttccacct gcccagtatt ccacacattg
    29341 tggtattgtt acgttagtta ttgtgtttcc attcaataaa aagcctgatt ccggtttcta
    29401 tattcactga tggattgtaa gccaataaat caacggcctt cgtaatacct gcacaagtgc
    29461 ttaccgactc tcctccgatt gcaggtaagt agttgatttt tgcgttttga ttcattagtt
    29521 tttcaaaact gtgaataatt ctggaaagtt gtacggggta attatttccc agattaaaaa
    29581 cttcgtagca ttctccttta taatccatcc ccagcaacaa tccatttata atgtcatcaa
    29641 tataagtata atctctttcg gaactgccat caccataaac cgtaagctct tttccggaac
    29701 aaatacaatt gaatatttta tccatcatca ggtcagggcg cattctacgg ccatatacac
    29761 taaaaagcct cataataaca atctcaatcc ctgtttgaga agaaaaatca ctaagaaact
    29821 cctcggcctt aagttttgtt gatgcgtatg gattcatagg atttgtagct gagtcctcga
    29881 gccaggggcg agtcggattg tttccatata cacttgatga tgaggtgaat acaattttgg
    29941 ggatttgcaa atgctcagct acgcgggcga tgcttattgt accatcaacg ttggctttct
    30001 gataaccttc cgggtgttga atagaatgcc tgactccagc acgtgcagcg aggtgaaata
    30061 tgcattccgt atttgcaggt atttccgaaa tatagccatc agggtggtta atatcattct
    30121 caataaagaa atatgcagga tgcttcaagg cctgagcaat atttcgtttt tttacagata
    30181 aatcgtaata atcatcaaag ctgtcaatgg caatcaccct aaaccctttg ttcagcattt
    30241 tgtcggcaag gttacttccg atgaaccccg ctccgcctgt gataagtaca gtgcgcattg
    30301 aattactttt agcaaatgta aaaaatatcc cgttaaaaag cacgcaaagc attatattct
    30361 ctgctatttg gccagaagta aatctttaac actgcactca gaccaaaaga atgcttttta
    30421 acaatccggg cttactgcag ctcaatcagt acatctccct tggcaacttt atcggctttg
    30481 gtaatatgta ttttttttac aataccggct gtatgcgatg taattatgtt gttcattttc
    30541 attgcatcga cggtgagtag ttgttgcgat ttagcaacag tatcgccttc ttttaccagc
    30601 acctcggtta ctgtacccgg tatcaaggca tatatggtat tcacatcaac aggttcgtat
    30661 ttctgacgtt ttaaaaagcc ggcagttaat ttggttttat aagaaccgtt ttcggtagtt
    30721 attacatggt attcttccat agttgtattc tattaaaaag gagggatccc gtgtttttta
    30781 tccacacgga tttgactttt tcctttacaa accgacagag cgtttatcaa tgccctgcgt
    30841 gtttcttcgg gtttaataac tgaatcgaca taaccttttc ccgctgctac atatggattt
    30901 gcaaaccgtt cacgatattc atccactttc tgatttcggg tagcatccgg atctgccgat
    30961 tcactgattt ctttccggaa aataatattt gcagcgccct gaggtcccat tacggcaatt
    31021 tctgcgcttg tccaggcgaa cacaaagtcg gctctgaggt ggcgtgagct catggcaatg
    31081 tatcctcctc cgtaggcttt tcgtaaaatt actgtaatct tgggaacggt agcttcgcta
    31141 tatgaatata atactttggc tccgtggcga attaccccgg cgtgctcctg atcaactccg
    31201 ggcagatatc ccggcaaatc aacaagtgta accaatggaa tgttgaaagc atcgcagaaa
    31261 cggataaacc gcgccgcttt gtcggacgaa tctacgtcga gaactccggc aagatacaga
    31321 ggctggtttg caacaaaacc aacagtttca ccagcaatgc gcccgaaccc aacaaccata
    31381 ttccgtgccc agagctcctg tacctcaatg aaaaacgaaa aatcggatac agctttaata
    31441 acttcgcgaa cgtcgtaagg catggaaggg tcatcgggca taataccggc aattttatca
    31501 gcattcaggg gctcctgcca ttgaagagca gcaggcttta ccgaattgtt tgcaggtatc
    31561 atcgacagca atttacgtat tgtatcgaag cattcggttt cggtcttcga aaagaaatgt
    31621 gcgttacctg tgatttcggc atgcactctt gcaccaccca gcgcttccat cgaaatttcc
    31681 tctcccagta ctgtttttat tacttcgggg ccggtgataa acattttcga aacctgatca
    31741 accacaaaca caaagtcggt cagggcagga gaatatacag ctccgccggc gcaaggccca
    31801 aggataacag atatttgcgg aatcacacca ctggcttgtg tattgaggta aaaaatttca
    31861 ccatagccgg caagagagtt cacgccttcc tgaatgcgtg caccaccgga gtcgtttatc
    31921 cctattaagg gaatccggtt ggtcagggca acttccatga ttttggtaat ttttcgtgca
    31981 tgcattagtc ccagcgagcc accggcaacg gtaaaatcct gcgcaaatac acacacaggt
    32041 gatccattca ctgttcctgt gcctataact acgccatcgc cgtgcaattc cttcttttcc
    32101 atgtcgaagt cgtgggcttc gtgtttgata aacaaatcgt actcctgaaa tgaagccttg
    32161 tcgagtaaaa gatcgattct ttcacgggca gttaatttac ccatcgattt ctgtttttcg
    32221 atagcttgta caccaccacc cagttgaagc ttttcctgct tcagtgtaag ttcatggttt
    32281 ttttctacat tattcataat tatcgattta attcataatt tggtctattt gggagcctga
    32341 aaatataacc ccaaagcaat caatgcatat aaaatattgg gaatccacac tgcaaaagat
    32401 gccgggaatg ttgcgttaat agcaaacatg tttgcaaatt tcataaacaa tacataagaa
    32461 aaactcagca ggattcccac gccaatatta attcccatgc ctccttttcg ctttcgcgat
    32521 gataacgaca cgccaatcaa tgtaaggata aatgtgctga atggggtcga tattcgcgaa
    32581 tatttttcaa tttcggaatt cactacatcg ggtgtcccca taagcttttg atcggcaata
    32641 taagcatcca actcattatg atccattgct tcaactattt cttttttctt tttaaaatct
    32701 ttcggactca aacgaagggt ggtgtcaata cgatttccgt atattagcga ttcttttatg
    32761 ccatcaaaat ttcggatgaa atatcgttga agaatccatt tattagttat ggaatcatac
    32821 agcatgttgt ctgcgcataa tttcgatttc agtttgttat tctcgaattt ttcaatggta
    32881 actttattgc ccatatttcg ggtaatgtta tagctctcga tatatatata aattcccgga
    32941 cttatttgcc tgtgcatatc aatggctccg atatgtgacc ctgacccttt catataggtc
    33001 tcctgaaatt caagccttac tttgttggac gagggaataa catagtatcc gagcacaaat
    33061 gaaaacgcgg taattatgct tgctgcaata aaatagggga taagtattct tcggaagctg
    33121 atcccactac tgagcattgc aataaattcg gagttctgtg ccattttcga cgtaaagaat
    33181 atcacagaaa taaacacaaa aagtccactg aacaggttgg caaaataggg tatgaagttc
    33241 aggtagtagt cgaaaaccac tgttttcaac gatgcgttcc tgtcgataaa atcatccatt
    33301 ttttccgaca ggtcaaatac aatcgaaatg gcaatgatca acgccagagc aaagaaaaat
    33361 gtccctagaa atttcttgat aatatagaag tcaattcgtt tcaacgcctt ttcattattg
    33421 tatttactac ccggttcttc caacttagga actctccggt atgtattttt tcgcggatgg
    33481 ttctcatcag ttttatatag aatccaagat tatggatgct cgctatttgt gctcctaaga
    33541 tttcgtttga aataattaga tgatgcaggt atgcttttga gtaatctttg tcgacaaaca
    33601 catctccatc ttcatcgatg cagctaaagt cgttttccca ttttttattt ttgatgttaa
    33661 tggttccatt catggtaaaa agcattccat ttctaccgtt tcgggtaggc atcacgcagt
    33721 cgaacatatc aacccccaga gctactgctt ccagaatgtt tgcaggagtg ccaacgccca
    33781 tcagatatct gggcttaccc gaaggtaaaa cctcattcac tgtttcaatc atttcgtaca
    33841 tcacctcatt gggttctcct accgaaagtc cgccaatagc atacccattg gcatcgtatg
    33901 acgaaatagc atctgctgac tgttgacgca ggtttttgta aacacttccc tgaattattg
    33961 gaaaaaacga ttgtacatat ccgtacaagg gttccgtatt gcaaaagtgt ttccaacccc
    34021 tttcaagcca ttgatgagtg aggagcatcg atttgcgtgc agcttcgtat tcgcagggga
    34081 atggtgtaca ttcatcgaaa gccatgatta tatcagcacc tatttttcgt tggatttcaa
    34141 ctacattttc cggcgtgaag atgtgctttg atccatcgat atgagatttg aatatagccc
    34201 cgtcttcggt aagcttccgg tttgcagcca gcgaaaaaac ctgatagcct ccgctatcgg
    34261 tcaatattgg gtgttgccat ccagagaatt tgtgaaggcc ccctgattca tgaatgatat
    34321 caattccggg ccttagatac agatggtagg tatttccaag aattatttgt gcatgaatgt
    34381 cgtctcgaag ttctctggca tggactccct tcacggttcc ggctgttccc accggcataa
    34441 ataccggtgt ttcgatactc ccatgcgctg tttctaacat tccgcaacgt gctttggtcg
    34501 aaatatcgta tttcctgata ctgaaattca tggcctaaaa ttgcccaaag ataaaaaaaa
    34561 cattaaaccc tagcagcatg ggtcaaaaat gtctatgagc atcaaacgta gcaagaagag
    34621 attgctagac ctgatttgaa tttttttaag ctctcaaaac aaaaaacttt caatgcagtg
    34681 ttacgggcta attgcaaatg gtaccaaaag caattaattc tttaacttat ttcatgtact
    34741 gaatcattac ccttatttta agtaaatcac ccgattctcc tgcatcctca agtgcaaccc
    34801 caaggattat tccgggttca gttgctttca tggctacacc gggttccgaa gaactggtaa
    34861 ccggatcccc tttttttatg ttactactct cattattaaa tctaaccaaa gcaactccac
    34921 ctgaaacgac agggttgctt tttgttgtgg cattttctga gcttttgttg gcggagtata
    34981 ttcctacaat gaattcatca taagggccag atgatagttt taactcagct ctgatggttc
    35041 cggtttcgct tgacaatatt gtgccatcag ggaaagtgaa gtcatatctg ctttgatgtt
    35101 gttgggcaaa gccattactt acaagaaaga acaacggtaa tatagcaaag aatgtcagaa
    35161 aaatgtttga atttattttc attgttaatt atttattcat taatcaaaaa ttgttaaaat
    35221 gcaatgatcg cccgagttag taaatgtaat tgggtttatt tctacccatg caccactttc
    35281 ccagaaataa cctgcaatag accactgaat ttttattgta tgggaaccgg gtgcaagagt
    35341 aaccggaatt ccggcaaaag caaccccacc ggaagtaact gttacttcgc cataaaaatc
    35401 atcaaaatca taatcggtta taactgaagc agcttttgct tgagcaaccc catcaacaac
    35461 aatctgtgcg ctaacaacaa tctggcccat tgcagcatta tgtgtacttg aatttgcaac
    35521 tcttgccgta aatgatgcaa aaacataaaa tgtattatgc acagttgtta ctgtcaacga
    35581 cattccggga atatcagtaa aggttgtgct tgttgttccc tgtattgttg tgagcttcac
    35641 gctatgtgca ttgtttccgt acattggcac agtggcaggg ttttgccaaa ccggaggtac
    35701 tccggctccc tgacttacca aaatttgacc agaagttcct gcctgattat ttggcataag
    35761 tgctccggaa aacttgacag cacccgcaac gtccagcttt tgggtggggc tggctgtacc
    35821 aatacctaca tttgtaccgt tgtcgtaaat ttggctgcag gttccagacg taccattcga
    35881 ttttattaca tagtttgcat tagtgcagcc tacaggacca gttgctcccg tagcacctgc
    35941 tgcccctgca ggacctgtag cacctgctgc tcctgcagga cctgtagcgc ctgctgctcc
    36001 ggcaggacct gtagcacctg ctgctcctg
//
LOCUS       DAOOQP010000016        34237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1466_length_34237_cov_5.790533, whole genome shotgun sequence.
ACCESSION   DAOOQP010000016 DAOOQP010000000
VERSION     DAOOQP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_34237_cov_5.790533"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLK75_03055"
     CDS             <1..401
                     /locus_tag="PLK75_03055"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HOY38254.1"
                     /translation="IVFDAQNEAYYKPSEDKIYMPLPKQFVSKEAFLSVMYHEASHWT
                     GAVHRLNRPILNKFGTKEYAFEELIAELSSAFMLAYLGYESRITDNAAYIENWLSVMK
                     NDTKFVIQAASQAQAASDYILQLANVEIAA"
     gene            complement(613..2175)
                     /locus_tag="PLK75_03060"
     CDS             complement(613..2175)
                     /locus_tag="PLK75_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38255.1"
                     /translation="MTNLNQNQKFNPNAGLREKSLSVIITMCAMLVSTVAFGQTYFVV
                     NWDDYTANSNVAYCTCDYIKCVPPEGVTGIYWGYPGNTEYVDTLLLENGFDGKVTCFY
                     DGGDKYLRLRPTFSPISPLFPTDIDTCVLAIDLDAGNYSQYGFTTYDWSNGANSQTIS
                     VGPGTYSVDIHNVCGDISASTTVHYDNPNAPDLGPDQEVCVGEQVILTTMVSDPVSTT
                     WYPGGSTSTDLEVTTSGTYRCYVVDANGCSGRDTVVITFIPTIDMEMCHVSFDTLTNK
                     NQITWFVDPMQEAITDVKVYVKNNLGQWVLLGTEDYQNGYFIHWESNPTADYNEYRIA
                     AVSECGDGEMSDIHRSIWLSTFQSELQWQNYYGTFVPDYYTVFAKMNTGELQQLGTVG
                     SCSGGGCLNHYVISQNPDIVKYFVAFEHDCSSNKDGENWVFSNNVDAEVGINTAESTS
                     FSIYPNPANESVIIHSEYSDYEVSIYTITGQQIMQERNCNTIDISDVAAGTYIVLFKS
                     GDTVSRRKLVVE"
     gene            complement(2350..3207)
                     /gene="rhuM"
                     /locus_tag="PLK75_03065"
     CDS             complement(2350..3207)
                     /gene="rhuM"
                     /locus_tag="PLK75_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOY38256.1"
                     /translation="MKNEIILYRPNELAEHIEVRLEDETVWLSLNQIAQLFDRDKSVI
                     SRHLRNVFKSGELVYDSTVAKNATVQIEAGREVKREIEFYNLDAILSVGYRVNSKQGT
                     QFRIWATRVLKEYLLKGYAVNNRINRMEENIYSLTKRVDEIDFQIKTSLPPNEGVFFD
                     GQVFDAHVFVSQLIKSAKESLILIDNYVDESVFMLLAKREKAVKAVVFTSNFTKQLQL
                     DLKKHNQQYLPIEVKTFTKSHDRFLIVDRKEVFHIGASLKDLGKKWFAFSKIEFDANV
                     FIENLNTNF"
     gene            complement(3288..3737)
                     /gene="rhuM"
                     /locus_tag="PLK75_03070"
     CDS             complement(3288..3737)
                     /gene="rhuM"
                     /locus_tag="PLK75_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOY38257.1"
                     /translation="MTNEIIVYQDNEQSVKLEVRIEEETVWLTQNQIVELFNSSKANI
                     SEHIKNIFETTELDEFSTVRKIRTVQIEGSRKVSRELVHYNLDMIISIGYRVNSIRGT
                     QFRKWANKVLKEYMLKGYAVNNRIDRLESDVHVQKKRKRKYLILLSS"
     gene            complement(4706..5455)
                     /locus_tag="PLK75_03075"
     CDS             complement(4706..5455)
                     /locus_tag="PLK75_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOY38258.1"
                     /translation="MINNTNKILLAIIIPVISISLVGAVYYFRAPIKTLMLKACRIIC
                     PPPDPPAIDSIRNTAWGIDISHHQKAVDWELLVEKNKPDFIFLKATEGSSHTDSKYSE
                     YAAEAREQEIPVGAYHFFSYNSSGKQQAKKFIKKARLSEGDLHPVLDVEFRKNMKNRK
                     WIIAEISSFCKEIKKEYGVNPIIYCEYDYYKKYLKDDFGDFNYWISDFYREPRCKYVF
                     WQYTDKGRVEGIGNIDNNRMNESEKLEDYIL"
     gene            complement(5536..6402)
                     /locus_tag="PLK75_03080"
     CDS             complement(5536..6402)
                     /locus_tag="PLK75_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38259.1"
                     /translation="MPGNLSQKEAFLYYLARMDTVMLDEILDDETPYFGVSKTVFLTK
                     LDFMFYLEKKRGKKQILKIKQHRKHKEIFYLIFKNSRYATKFIMTDDGEKILSLQQAF
                     SMKERDGIGDSLFLDLTFYSDEMTDFVPTADYLEKVEKCQQAVDEFFSPLPKFKTSKH
                     ISDWIYKYTDLHVQIYDDYLMKKFHEFKVLYYYIGFLFEYISHYPEAKRALETYDSSD
                     PYYLSLWLGHYFKLNAFHLVGFGFDFKRVDHTKKIITYRRFHKTCYFIGDDFFTMLEF
                     INLYAKYTDKIY"
     gene            complement(7345..7809)
                     /locus_tag="PLK75_03085"
     CDS             complement(7345..7809)
                     /locus_tag="PLK75_03085"
                     /inference="COORDINATES: protein motif:HMM:NF039475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6508 domain-containing protein"
                     /protein_id="HOY38260.1"
                     /translation="MSKLKNTSMHLNENNYIETINGFNSSDWKPILDLIPEIEQTSTF
                     GEMKGGEKNAEGIMVMPYWIESPVVSRFHRIVYDMPIIIDFDWGSWGEGRKMVRDENF
                     DFNSIDIPTKCKIITAIVRSNRFCDGALVSAFESGLILKILKSIRQQTGAGD"
     gene            complement(7847..8173)
                     /locus_tag="PLK75_03090"
     CDS             complement(7847..8173)
                     /locus_tag="PLK75_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38261.1"
                     /translation="MFSSNYANYIIKAQIDEPFHFDALNLIKLVPTSGILNLTQTEIA
                     ERLHISEEQFASLFEVLNKHKNQLIRYENSAYIFDYPEEGINYVKHFSRAMSNMGMSH
                     NPDFFE"
     gene            complement(8185..9048)
                     /locus_tag="PLK75_03095"
     CDS             complement(8185..9048)
                     /locus_tag="PLK75_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOY38262.1"
                     /translation="MIADWQTETIYFSEWLRKLQSYKPVCRFIENELTARHISYEFLQ
                     GTNDIWARDYMPVQISDEKFIEFRYDPDYLQGTAKCRRNYKTYPDIVCQHHSIPTLKS
                     GLIVDGGNVVKSSKAVIMSEKVFAENRHNFSKKAVIDELEALLETEKIVFVPWLEKQT
                     DECGHTDGYVRFIDDDTVLVNHIYRNTSRLLKPLQLAGLRIEKLHFDVAREHRHNWAY
                     LNFLQTRDLILVPQLGADEDEQAFRQLSDYYSRYAATNSIVFVPGMLPILDEGGALNC
                     ISWTIQNNKTN"
     gene            9540..10136
                     /locus_tag="PLK75_03100"
     CDS             9540..10136
                     /locus_tag="PLK75_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38263.1"
                     /translation="MKNTIFYVLRGEFYRTQLDTNNLVKIYKTFASNKPLHARFAAFQ
                     SFHDYIELLYLAKNKRYSNYKQAVVDLQDFFNSYSLDDILTGNDSDTFRGISLSMVID
                     PRVEYVSSERITYYNDEIEIHGFSKNSNDFSIQREYFNNLRREAEIYKQRGIPTGVNT
                     AKYDISPLFEDPEIIEILPTPINFNDMISDLLIMGERV"
     gene            complement(10148..11386)
                     /locus_tag="PLK75_03105"
     CDS             complement(10148..11386)
                     /locus_tag="PLK75_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38264.1"
                     /translation="MTDLRNYPFPRLESGSAKNIEDFFEKIIRQQFAKKAAIVKLHNA
                     LMEYVKSDKPTLFLRLYGSAKNYDLLRRGFVTEYRDGTKLVFCDNTFSMYFTGLKIAG
                     IEYTRNDLADYLNKKAIIVSFGLTSNEKELAWYNPSGALNIPFNALGWYQAHIKPTGF
                     GLGDFGENLMSYFPNPNRTEWGNDKIRRINENLSADQRTLLTAHFVRLIHPLNSVLVP
                     KNNNVIYDGLRLGEETELLEFVRNYLRNEFKKEYEEFETISLAYQAPEIQHTLGLIEW
                     FDKSVVKPKKKKQQSKKSAKAGIAKTPKIKTKEIGEETRVEKLDHWLKSIGKTAFIEI
                     FYPELSKDENTSLETFEKKYEKIREFKTPHTRISSAKSIIREGLAEEALLLVINSKKL
                     DPHILETAQKYLDDFRSNKN"
     gene            complement(11502..12320)
                     /locus_tag="PLK75_03110"
     CDS             complement(11502..12320)
                     /locus_tag="PLK75_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38265.1"
                     /translation="MENTFTSEFDRGILSKPLFLESPVTQDFVSWIKPYINGEKSLTD
                     LNYPYHCFTEAVNNYYWGKSVKEDFDRTYNRFAKYRDDFKSQNESIIRKNCNDVLNWG
                     GVAAKNQDKIAVRDSLYEFIQAVLNLVNHDKIIIDDLNPNYINSGFTKIYAASDENYI
                     MYDGRVGSALCYLIKNYLDKKGISLLPTELSFGYGIGRGNHQRNPSTSTDSFKFPEIT
                     QNRIMHFISNIKANWLAEAIAGTGDLFGQKIKAKNIFAFQTALFTLGEEIPKSK"
     gene            complement(12332..13030)
                     /locus_tag="PLK75_03115"
     CDS             complement(12332..13030)
                     /locus_tag="PLK75_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38266.1"
                     /translation="MNTTSSPITQKFQSFCETIYNADYADKNYNHFDKLMDYVMNRLC
                     LVVGETRYRIVEAEVYYKSIKHPDPYTHSVPEQMTQGGWYFNGMGLDITFGRKAKTVD
                     ENIYGGILIRGIRKLDGENQYITGVSNCLKVLFSSIQHISDGRPSFYFVENENEYDIP
                     KYEPVKSTRMGLTKKSDDEDNYWERPYRYIVELKEAHKFRQKEDVVKGLLQKGTIDLE
                     LAKEMLGYQLKSNS"
     gene            complement(13040..13861)
                     /locus_tag="PLK75_03120"
     CDS             complement(13040..13861)
                     /locus_tag="PLK75_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38267.1"
                     /translation="MYNQLLRKELTALAHKYGSKNYGQAYAHTLIKSTDAVIFNDVSY
                     NFHKESWKEIFGNSSYLDRTKKIHSHLKSLPCPAIYEMQSSNSSDALTMNIFCHPIIK
                     TWAGVCKLLKVNSLSAIEFGVNGNVKKIKNGVIISDTTEIDIRINNSIICECKLTEKD
                     FTSHNKTDVERYLSFEQVFHKDKLLQTQTEYKNYQLIRNILAARQIEGGRFILFCDMR
                     RPDLAQSFFHTVNCIQDRYIDLRTNCEIIYWQDVAHVVGKDLKDFLEEKYGISSY"
     gene            complement(13950..14903)
                     /locus_tag="PLK75_03125"
     CDS             complement(13950..14903)
                     /locus_tag="PLK75_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38268.1"
                     /translation="MKHNTHIYLAYKAIEFLYEGSSNIIDSKTGNAIIEKRKKALRIT
                     AKELQRMLMHYIEFIDEATWAPDDIINDKAIYHTFKLFTDQEYPGSRSFAKEVHFEKY
                     YRASGGGGLPYKVDHLARVISDMIKLRLYNDRFSMNQIMYQYLLISHYIVDANVPMHC
                     DLRDDPPEASDTTKPLNGNYFKESLHGNVEQLWDDAVTPVAVSEEIIKPSRTSDKPVK
                     TELSEAIIFDIFNPAHIGLIKPELIAENDLMEFMLKKCVSTKEKSLMLFPFDKPEEWG
                     NESMNRVKAETRQIFAGAIADLISVWLWIWMKNNKSKNTDL"
     gene            complement(14949..18236)
                     /locus_tag="PLK75_03130"
     CDS             complement(14949..18236)
                     /locus_tag="PLK75_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY38269.1"
                     /translation="MWKVIITSFILIGVYSAVLGQNNYMKMIDDGKHAKAEKKLNKAI
                     EKSSDDIELNYTMAYLYISREFNKYNPEQSYEYLTKAYRKFNQVNDEKIIKQLEKIPI
                     NKSKFESLLDTVSRCALEDATAINTIVKYERFLSYFELSPKKYKNSAIERRDVVAYAE
                     ASKINTVESFQSFMNNYPVAKQFRDAEKNRNRLAYKNAEKENTVSSFQRFINNYPNAS
                     EIPDAIKNRDKLAFEEAKRIDNSNAYKEFIKKYPDSKQINDAQFLFETRLFEEETDSG
                     NEYSYVWFIENNPDNRYVKNAIDSLYLIGSEKKFLPLIAYGLSKSKGEQYNKGVISIY
                     DLLSEDGELSSLERFEYEFSGYMNLIDSFDIDLQNARYAFNIGITSSVNDSYYNNSDE
                     MNQRLIREGAKTGSIQISLMWDNYNDLDLHCIDPFGEEIYYNHKKSISGGELDVDMNV
                     SPNSLSPVENIYWENGKAPLGQYTIILDHFKNHRCGNYCKDPTKYKIRLKVGNSIKEF
                     SGSISASQPNRTIHSFNYEGEVFGEIELNQDNISKLKQYIIMAAPNDLAFVALQILIS
                     NSIKYKKWNEAITTVKQFENYFTNDSKYSDLLSILQSNSDNTIQINSIPLVNTTNGGE
                     YSPVISADNSSLYFCGKDREDNIGGEDIFISKRKGNNWDKPELDRFLSFRNSNDAPMA
                     LSSDGNKMVQFKDGKLGYSEKTEVGWSEIEYFPDNVNTGDWNGDAMLTSDGNALIFAS
                     ARNGGFNYTPIGLSGYHCDNNYASDIYISIRNGNSWGEPINLGHIINTVYSERSPFLH
                     PDMKTLYFSSDGLGGLGKYDVYKTTRLSDTCWDCWSDPINMGKEINTAESDWGYKVST
                     DGSKAYFSKKDQNSKKNDIYWIKLPNHLRPEMVATVSGRLVDADNQPVSADIRWEDLT
                     TGKNVGQSKSDPVDGSFFIVLPLGKIYGYYVDGDEYFPISNNIDLRTSTEPVVIEENI
                     DLVSFKQMIEEGTAVPVNNLFFNFAESSLLPYSLPELQRVAGIIKKNGLKVEISGHTD
                     NIGEEENNQKLSEKRAEAVRDYLINEGCDPSMLQTIGYGESKPIATNDTDTGRAKNRR
                     VELKFVK"
     gene            complement(18855..20306)
                     /locus_tag="PLK75_03135"
     CDS             complement(18855..20306)
                     /locus_tag="PLK75_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOY38270.1"
                     /translation="MQKVRTIQAMKGQAMFKEMNTYRLPEQEEDFIIPQSVKNFPSEF
                     HPDVYRYLPKALSDACSQFSNPKEKDIILLGALSVISGCLPFVCGMYDMKTVYPNFFV
                     FIEAPAGAGKGILQWTRKFAEFIHKDMLNTSAKAYEAYQSELRLYRNSLKKKTKEPLK
                     EIPGEPARKMLIIPANNSASSFLQTLSENDGAGLLFCTEADTLANSLSQDWGNYSDVL
                     RCAFQHEPISLQRRTNREYLELSEPRLSALLTGTKDQLFKLIPSTENGLFSRFAFYSF
                     PAVTVFKDVFIDMEHRTLRIFNELGQQMFEYRNRLISLGSTIEWQFNTTTRAEFKSCM
                     NDIMQQLHKEIGDQCLATVKRLGLILFRIAMITCTLRVLESGKKPEPQMTIEHLDFGI
                     AYEIVVALIHHAITLIAETRTGNKNIVKQRRLNQYYDLLPDSFTRKQALQLAEKLDLS
                     PASCDRFLSSNIFTKPSYGNYTKSGDQNDKVIK"
     gene            complement(20257..20574)
                     /locus_tag="PLK75_03140"
     CDS             complement(20257..20574)
                     /locus_tag="PLK75_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY38271.1"
                     /translation="MKIIITTKKSLQAVIRNVIETLLDERLATQPNPNTEHPLDAYLN
                     ANEAASFLRISLTTLYGLSSKKQIPSIKQGKKLYFSKQDLIQWLENAKSQNHSSDERA
                     SHV"
     gene            complement(21115..21237)
                     /locus_tag="PLK75_03145"
     CDS             complement(21115..21237)
                     /locus_tag="PLK75_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38272.1"
                     /translation="MNLFAIRFHNTLYLIEKGSPGVSLDAAFKYYERYASKTTF"
     gene            21469..23118
                     /locus_tag="PLK75_03150"
     CDS             21469..23118
                     /locus_tag="PLK75_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38273.1"
                     /translation="MKLFSQELSTIKENKPISFTGALNTSGTVYFTSDDNPQRDPFIW
                     NVSGNFNLNIYEVFDIPFSFVLSKENKTFKQPSYQQYGISPSYKSVTLHLGYRNLNFS
                     KYTMSGITFLGAGIEFSPEKSLFKCKAFYGRFARAQQFIPDNMFNPQATFVMPSYERW
                     GYGTMITLGKKDHLFDIVLFKASDDPASSAIPDSLSVYPAENFVVGLNSKNNILKNLF
                     LEVNYALSAFSTDIRQGEIYQETYTYSNNLGFLFTPRSSSQFNSVIDVAINFKPGKVN
                     IGAKYLRIDPEYKSLGTAFINNDVQEFTGNISTTFNENRISLSANAGLQNNNLDKSQL
                     ATNKRFISALNYTWLISKNLNLTAIFSNYSTNSMPVQVYMIDSVKYSQVTRNASLNIT
                     ETFGDTIIQHSLSGMFSFQGGNTLNQTGDAVSDVSNTFANAYLAYRLNYLPIGFSVVA
                     SLNYSNFISGDINTKSIGPSVGVNKFFLKRKINTGINATYMNSFGTNANSNQIINLRL
                     FANYKINKYHSFTLSVNNLYKIASGASDHHSQASIAYNLIF"
     gene            23134..27948
                     /locus_tag="PLK75_03155"
     CDS             23134..27948
                     /locus_tag="PLK75_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38274.1"
                     /translation="MKKTLILVFILLSIAGFSQITNPVTVNLLLTPPHSAMLNEYTTP
                     GSDKIIANIRLNDLNEPMWNVRLKISLESNMVRISTKANYRPPTPVELLPGSVIQVSG
                     EDLAPYLNYANIDVQGITMYELQQNGILPEGFYTFSIEVYDYNSNRLLSNTANFNAFI
                     QLNGVPLIQTPVTGSVVMHSSPLYIPFQWQLSSPSIYGDPSTIEYQLSLYRIIDPNVN
                     PVNSIINNVVEKVYESNFSNATSLVYGINEPPLEVGEKYSFTVQAQDAEGRDIFKNKG
                     FSEVGWFYYGYPDGGHISIVAPENGKNFTLSEHRHFEWGAPDNVLSDQQVYYYLKIVE
                     LAENQDSIDAINNNVAWTEFTSIPQIGSNGFAYTLTKAPETQQEYVWQVKAFTGDQEI
                     ASSDVYKFIGPPVIERFMAGSHEVIVSQTYNQDLSNLSGKGKVKISEAGNEVEFEFEG
                     LQISNAGGRSVLSGGELLAELENFDNIQLSANDTDNGTAVFYADSIKLNKEELAIKGH
                     FVWPFVLVTDAPDLANIVSSSAWVNYDVYKLLGSISLGNNNSFTLIDPIGYEISFDST
                     ANFLISENRFTPRFYGKLTLPTSVKNTNNERMVLPFRNVNNLFYFNQENVQAEHKIKI
                     AENANLAFIPLAYTVDFSNVDAIDQIGKDAMWKGVGFEKAKIEIPVNVDMQQRINPEY
                     PMQYVFDIAGTPNQFAYIDNGGMTASLETNFSLYNAGNYCGFAINITKLKLLISGNEH
                     TNSELSVATKIPLLGNDEMAANIIIDANGFQIMDLNTQLLNKQIVLNADKEKLKLILT
                     IKQAVFKDNEKLEFTVNMDWPYMQTYIPNLSGLCVWGNDDFGFNEPNGACILSNPVSA
                     KYYNTYDLIVDSIVSGRHGQNYMMGVNGEIVLADNIAGANNKPPRVTMASYELKEGAS
                     STYDLSVETWVQSFINKAEFLSTPENIFANLPYIRVNTPVIEFEGGLVATNEDPEWGT
                     AFYALLDGTIKQPQEYHAKVQFLIGKLDGEPYWFAELGLSTSGHEHDQAQVPEGKKKK
                     LKKVQMSKSGIKIGPLEINGITGRVYYHMRPETAVGVDCNMKFDEIAEPGLSNYHLGL
                     PDLPDADVCEILNHLNAAQMKSLLCSLHSSAFNAVLEKFPEPNFDDIQSYIESHGSSD
                     SEFLNQMIAAEARGRMQDAYENGGIQDQYHKFTYDRLAKVFPGYDWCQKVIDDNDEGM
                     QWGNLLCQMPNIQWPRFPDVCELSEYFFNRVLEELPPPSYDDLNAIMYDENWDMIQVD
                     HPNITWPDIHEIFPEKNLCKFIMIWPGINWGNIYVNIPELPKLEWPDWEYYFPGLPDL
                     EVVLPELNIEVPSISFEPGEIEVDYKVDPSVSYGVYVLVNYVDFANKGEVVEGTGTME
                     VKFTDDGGLEDIGFEVTSNWGNFAGAPPVIEGLGCMHYSRDANRFVGDFFGQARNPAV
                     CGHGHLHIDINPDFWEINLASRENPVVVQPLCSNGPVRFTGYFLLNQSRFEIGLGASM
                     GAEFGGSISTEVCSLGIKAEISLKADILASIQYKPNIKINKAYFGVDFHAGLYVTTSG
                     TLCDFGNFELASIGLKGQLEYYERYLSGKVSGHATFLSIVTCDFDLSTGINL"
     gene            27954..29933
                     /locus_tag="PLK75_03160"
     CDS             27954..29933
                     /locus_tag="PLK75_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38275.1"
                     /translation="MKNKTAIVLIFLLIFSHQLLAQENAKVIVSNTDNSEIWVKWYSE
                     FIYFDSPVFLYRQENGRSTWEFIGEFSRGNGMSREILQEDKILEVLQNSVMGSRAGDL
                     DGMTLLILMIKSVEHPELCQFLGIQHIDKTVTAGKQYRYKICKGNSPEANSLAISDYI
                     TSTKYEKVDPPADIKAWQDNYSAKFSWRPNPDAFMGVKMYRASDNEDMELITPVPIIH
                     NLDENGNYQEALFTDDSLVLGKEYRYIVKSIDYFGRESKPSSEIIVKIGDVSPPLPPH
                     RLGVSVIGKQVQVYWENENISDLAGYRIYRALEGNTDYIAVNSSLIPKEIQTYYDTVS
                     TIGLYNYKVATVDTAGNEALSIAYLADIKDVFPPAAPQNLKASSDTGIIHLSWDICPE
                     PDLMGYYIYRSMNGNPQTYMLLNADHYTQNNFSDKLPKQISSNFSYKVVAVDSSYNKS
                     EFSNTAFTKLPDVTAPEKPVIKTITRNNNTLIIQWYSNYENDLLGYNIYRFTENQHPD
                     QAEKINLDVITGINAFTDRFAEYETSYRYIVIAVDKQGNFSAPSEAYTAPRLAYPDAN
                     LSFDKFRLIHKNNKKQVIIEWKINKPEETKAYVVYRKSESQKHFTPLSGMLTESSYQD
                     MMKNENGTYQYRVGALGKDDKMYYSEPQEIIILKK"
     gene            29938..30948
                     /locus_tag="PLK75_03165"
     CDS             29938..30948
                     /locus_tag="PLK75_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOY38276.1"
                     /translation="MKTLLLIASLLLAVNLAFSQVGINNSSPDSSAVLDLKSNSNNQG
                     LLVPRLSSAQIAAISNPATGLLAYNTDWSLFCYYDGSNWYSLRTFRQILNWGDLEPPQ
                     DMDADIYNGKNMGIGYSGANSKISVNGNMSIGANEEAPANGAFILGQVRIGSNVGFKK
                     LEVDGEIHSTNNIDTGGKIKEGGNDLLPAGSIIMWSGSTPPAGWKLCDGTNGTPDLRG
                     RFIMGYGAHNEKTLKYDGDSTLTPVNPSIGPKGGYDKVAISESQMPSHGHSVYITFTD
                     RYTNLEESGLRNDGTHENGDNRTFTVWLDRIFNVTTGNNGGNVAHENRPPYYVLAFIM
                     KE"
     gene            30960..>34237
                     /locus_tag="PLK75_03170"
     CDS             30960..>34237
                     /locus_tag="PLK75_03170"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY38277.1"
                     /translation="MHTLNKNIKLILSIAILLSCKLIAYAQNTQDTITIISNSEITIL
                     NDSVFIKGCVFDKTNYSYPYQGSIFNSGYVSITGDIVNNSGISLVFSMEEQGQVHFVG
                     DSLQRIMGNNVQFNIVYIKKSGDTLYLDTEIIQNDSLLFHGGIIDLNGNDLFLSDYDL
                     HHKGRLGQESDSSHIMGLTGYVYTQANMSSFDGYGMGIKFQNGSTAGNITIQRGHTSE
                     TTVTDGSVKKYFNLMPGQAGNHDVIVHYLDTTDLWDVNCNESDFKLWSSYTNGYYYEK
                     KYGGVDTTGNVAETNGEVIYIQNPTRITVADHICDVIPVVNIGDTLHICNGSTGSINA
                     YNNGSFFVWSTGETSQIISVNTAGMYYVTVTDPRGCFNIDSVLVVLDSVPHTNFSFNS
                     PVGPCPGTLISFNNTTTVAPEAEPLSYLWQFGDGNTATEEFPQHAYVAGGTYTITLTA
                     IAANSCAISHTATVHVFDLPEMSFTFNNACKNKPVILTNTTPNAFPGLTHWNLGNGDQ
                     FFAEDTAYFYTNHGTYAVQLIATDNHGCTDTITQNIEVYPLPIAGFTTDDADVCLQNS
                     SVFHNSSSIPYGNLSYQWQFGNGFANTTPNPIIVYDTHGNYTVQLIATSSYGCSDTAY
                     NNVIIRPIPNAEFSFSNVCEGDTVFFTNESSIDPSEILTYHWTFGDGSSSNIANPKKL
                     YSNPGSYTVNLEVTSEQGCSESIQKTVQVYANPNANFLCQPVCFGNTSMFQNMSLPNN
                     SSLTFYWEFGDGSQSDASNPQHYYNAEGEYLVSLIAESGVGCSDTIDKIVRVYPTPVV
                     DLGDNIFHCFNSYVLDAQNSGANYIWSNNAASQTITVNQNGDYSVTVTNNYGCSASDQ
                     VHVTLNAPIVVSFDGQYWEVCDSTTLNAGYPGADFIWSTGASTQSITVLESGIYAVTI
                     TNQGCTGVGSVEIVVHQSPQLNLGDVVTECEGLAVVLDAENEGSNYLWSTNQTLQQIE
                     VNQAGTYSVTVTDINSCTVSDSVQVVFNSLPIDPFPADTIICGTGFLNAHNQGAAFVW
                     NTGSTAQAIEISQSGNYWVTITGGNSCSLIDTVSVTAYPRPLVDIGNDTALCNEEILV
                     LSAG"
BASE COUNT        10128 a         6909 c         6491 g        10709 t
ORIGIN      
        1 agattgtgtt tgatgcccaa aacgaagcgt actacaaacc ctccgaagat aaaatttaca
       61 tgcccctgcc gaaacagttt gtaagcaagg aagcgttttt gtcggtgatg taccatgaag
      121 catcgcattg gactggcgca gtgcaccgac tgaaccgccc gatactgaac aaattcggga
      181 cgaaagaata tgcatttgaa gaattgattg ccgagttaag ctccgctttc atgcttgcct
      241 atctgggcta cgagagccgc atcaccgaca acgcagccta tatcgaaaac tggctcagcg
      301 tgatgaaaaa cgacaccaag tttgtcattc aggcagcctc acaggcacag gcagcatccg
      361 actacatttt gcagcttgcc aatgtagaga tcgcagcctg acacgaaaat caacacggtt
      421 ccggtccccc gatcaggaac cggtttcgct acaccccgac ctccgcagcc actgcacccg
      481 acctgtggct ttttcgcaag aacaaaaaag agccctttac ggctcttttc cttaaaaaat
      541 cacaacacac aaaacaatgg cacttcgaga acagttcgac aagctcactg tatcacctca
      601 gtgaccttgt ttctactcca ccaccaattt cctgcggctt actgtatctc ccgatttgaa
      661 aagtacgata taggttcctg cagctacatc cgaaatatca atcgtgttgc agtttctttc
      721 ctgcataatt tgctgaccgg taattgtgta aatgcttacc tcgtaatcgc tgtattcgga
      781 atgtattatg acgctctcat tagccggatt gggatatatc gagaaactcg ttgattctgc
      841 tgtatttatt ccaacttcgg catccacatt gttggagaat acccagtttt cgccgtcttt
      901 gttgcttgag cagtcgtgtt caaaggctac aaagtatttc acaatatcgg ggttttggct
      961 gatgacataa tggttgagac aaccaccgcc gctgcaactg cctaccgtgc ctaactgttg
     1021 tagttcacca gtattcatct tggcaaacac tgtgtaatag tcgggtacaa aggtgccgta
     1081 atagttttgc cattgaagtt ccgattgaaa agtcgaaagc catatactgc ggtgtatatc
     1141 cgacatttca ccatcaccac attcacttac ggctgcaatg cgatattcgt tataatcggc
     1201 ggttgggtta ctttcccaat ggatgaaata accgttctgg tagtcctcgg ttccgagcaa
     1261 cacccattgg ccgaggttgt ttttaacata tactttcaca tcggtgatgg cttcctgcat
     1321 cggatccacg aaccaggtga tctggttctt gttagtcagt gtgtcaaagg acacatgaca
     1381 catctccatg tcaatagtgg ggatgaaggt aatcaccacg gtatctctac cactgcagcc
     1441 gttagcatct actacatagc agcggtatgt gcctgaggtt gttacctcaa ggtctgtaga
     1501 ggtgcttcca cctgggtacc aagtggtact tacggggtcg cttaccatgg tggtgaggat
     1561 gacttgttca cctacacata cttcctgatc cggaccgagg tcgggagcgt tggggttgtc
     1621 gtagtgtacg gtcgtactgg cagatatatc tccacagaca ttgtggatat ctaccgagta
     1681 ggttccaggc cctacgctaa tggtctgtga gtttgcgccg ttagaccagt cgtaggtagt
     1741 gaagccatac tggctgtagt ttccagcatc gaggtcgatt gcaagaacac atgtgtcgat
     1801 gtcggtgggg aaaagtgggg aaatagggga gaaagtggga cggagtctta gatatttatc
     1861 tcctccgtca taaaagcagg ttaccttccc atcgaagcca ttttccagga gtagagtatc
     1921 tacatattcg gtatttcctg gatatcccca gtaaattcca gttactcctt ccggcggtac
     1981 acatttgatg taatcacagg tacaataggc cacattactg ttggcggtgt aatcatccca
     2041 gttgactaca aagtaggttt ggccaaaggc cacggtagat actaacattg cgcacatcgt
     2101 tataataacg gaaagcgact tttctcgaag ccccgcattt gggttaaact tctggttttg
     2161 gtttaaattt gtcatatttt caatgtattt tattaattat tcattcaaaa aagtcgttat
     2221 ttcgcaatcc tttccgcaat ttcccatttc cgagccttaa ttctccgccc aaattcgagg
     2281 cttcgggttt ctccattttc gagacttagc aaaatcccga attcggtact tgatagatag
     2341 tatgtttttt caaaagttag tattcaaatt ttcaataaag acgtttgcat caaattctat
     2401 tttcgaaaat gcgaaccatt tctttcccaa atcttttaag gaagctccta tgtggaaaac
     2461 ttcttttcgg tcaacaatca aaaagcggtc gtgcgatttg gtaaaagttt ttacttcgat
     2521 tggcaggtat tgttggttgt gctttttcag gtcgagttgc aactgtttgg taaaattaga
     2581 agtaaaaaca actgccttca cagctttttc tcgttttgcc aacaacataa atacgctttc
     2641 gtcaacgtaa ttatcaatga gtatcagcga ttcttttgcc gattttataa gttgagaaac
     2701 aaaaacatgg gcatcgaaaa cctgtccatc gaagaaaacg ccttcgttgg gcggcagact
     2761 ggtttttatt tgaaaatcaa tttcatcaac tctttttgtc aaggaataaa tattttcctc
     2821 cattcgattt attctgttgt ttacagcgta tccttttagt aaatactcct taagaaccct
     2881 agttgcccaa atacgaaatt gtgttccttg ttttgaattg accctataac caaccgatag
     2941 aatagcatct agattataga attcgatttc tctttttact tctcgtcctg cttctatttg
     3001 aactgttgca ttttttgcaa cagttgagtc ataaacaagt tctccgcttt taaaaacatt
     3061 tcgtaagtgc cttgaaataa ctgatttgtc cctatcgaaa agttgtgcaa tttgattaag
     3121 agaaagccaa acggtttcat cttcaagccg aacctcaata tgttcggcaa gttcgtttgg
     3181 gcgataaaga attatttcat ttttcattgc caaatttttc cttttatatc aatcctgatt
     3241 aattttattt atgaacatca tacttttcaa ctctttatta tgttttatta cgaagataat
     3301 aaaattaaat attttcgctt cctttttttc tgaacatgca catcgctttc gagcctgtca
     3361 atacggttgt ttacggcata tcccttcagc atgtattctt taagcacttt gtttgcccat
     3421 ttgcgaaatt gagtacctcg aatggagttc actctatatc caatagaaat aatcatgtcc
     3481 agattgtaat gaaccaattc tcttgagacc ttcctgctgc cttcaatttg aactgttcga
     3541 attttccgaa cagttgaaaa ttcgtctaat tctgtggttt caaaaatgtt tttaatatgt
     3601 tcgctaatgt ttgctttgct gctattaaaa agttcaacta tttgattttg tgttagccaa
     3661 accgtttctt cctcgattct gacttcaagc tttacagatt gttcgttatc ctgatataca
     3721 attatctcgt ttgtcatttt tttaatatag ccattacatt tttataaaca tccatagcat
     3781 cagtttcagg catagttcat attcagataa tcaattcatt cacaaaactc attaaaattt
     3841 tctcgtctgc gagataaata tggttgtatt ataatctgtt ccaaacttaa aaattacgat
     3901 ttgtttgtgc aaacgaaaaa atagaaaatt tgttgattat gataattttc attcctctgt
     3961 attattgaaa tttatattat ttgcaagcat ttgatcaact ggggcctcga ctggggcctc
     4021 aactggggct tcatgcgtaa atgtttcaat ttttccatct ctttcttctc ccaaattcca
     4081 ggcattgggg aacgacatgc gaataaaatt ctccgtaaat gaaaaacaat cctgcgtata
     4141 cgcctgcaat atacggggga tgcctgagcc aagatgctct accaattcca aatccttgaa
     4201 aatataattc caaaagtgat ggcatgataa aatgataaaa tgataatttg ataaagtgat
     4261 aatctgataa agtgataatc tgataaagtg ataaactttt ttcgaaaaat catcgcaaag
     4321 caaattttca caactttctg ctatttacac acgaagagaa tcacacgtat acagcataaa
     4381 cacccataat acaggtaagg cgggtgatgc ggaaacttac tgctattttt catgtattat
     4441 tgctattatt tttgagtgcg ttttacagct gtgcgcatac agtgaaagcc ttggtattac
     4501 ctacaactat tcaataacgt cttcagaaga tgtctaggac tactctagaa catttctagg
     4561 acatttctaa aacatttcta ggataagcca gaaataagtc agggataagt aagaaataag
     4621 tcatcatgga ttgaacttat tgacttccgc agccattaaa accgagccgg tggctttttt
     4681 ggggggatgt ggattaacac tgtcttcaga gaatataatc ttcaagtttc tcagactcgt
     4741 tcatccggtt gttatcaata ttgccaattc cttccaccct gcctttgtcg gtatattgcc
     4801 aaaatacgta cttgcatcgg ggttccctgt aaaaatcaga aatccagtaa ttaaaatcac
     4861 caaaatcatc cttgaggtac tttttatagt agtcgtattc acaatagatg atgggattca
     4921 cgccatattc cttttttatc tctttgcaga atgagcttat ttcggcaatg atccatttgc
     4981 ggtttttcat attttttcga aattcaacat cgagaaccgg atgcaaatcg ccttcgctga
     5041 gcctggcttt ttttataaat ttctttgcct gttgtttgcc cgaagaattg taactgaaaa
     5101 aatgataagc accgaccgga atctcctgct ctctggcttc tgcggcatat tcactatatt
     5161 ttgaatcggt gtgtgaactt ccttccgtcg ctttaagaaa gataaagtca ggtttgtttt
     5221 tctcgaccaa caactcccag tcaactgcct tctgatgatg cgaaatgtca attccccatg
     5281 cagtgtttct gatactatca atagccggtg gatcgggagg tgggcaaatt atcctacagg
     5341 ctttaagcat cagtgtttta atcggggcac ggaaataata cactgcacca actagactta
     5401 ttgaaatcac tggtattatt atcgccagta gtattttatt ggtattgttg atcattcttt
     5461 ttattcaatc taccatgttt tgtttctatt ttcctgtaaa aaaaaatcat actactccaa
     5521 aaaacaaaat taaccttaat atattttatc ggtgtatttc gcatacaaat taataaattc
     5581 caacattgta aaaaaatcgt ccccaataaa ataacatgtt ttatgaaacc gccgataggt
     5641 aataatcttt ttggtatgat ctacacgttt aaaatcaaaa ccaaagccta ccagatgaaa
     5701 tgcattaagt ttaaaataat gacctaacca aagggataaa tagtaagggt cggaagaatc
     5761 atatgtttct agagcccttt ttgcttcagg gtagtgtgaa atatattcga acaaaaaacc
     5821 gatatagtaa tataatactt tgaattcatg aaacttcttc attaaataat catcatatat
     5881 ctgcacatgt aagtcggtgt atttatatat ccagtcggaa atgtgtttgc tggttttaaa
     5941 tttgggcaac gggctaaaaa actcatcaac agcttgctga catttttcta ctttttcaag
     6001 ataatcggca gtaggtacaa agtcagtcat ttcatcgcta taaaaagtca aatcgagaaa
     6061 aagtgaatca ccaataccat ctctttcttt catagagaat gcttgctgca aactcaatat
     6121 tttttcgccg tcatcggtca tgataaattt tgttgcatac cgagaatttt taaaaatcaa
     6181 ataaaaaatt tctttatgtt ttctatgttg ctttatttta agtatttgtt ttttccccct
     6241 ctttttttcc aaataaaaca taaaatctaa cttggttaaa aaaacggttt tactcactcc
     6301 gaaatatggg gtttcatcat caagaatttc gtcgagcatt acggtatcca tccttgccag
     6361 gtaatataaa aaggcttctt tttgtgaaag gtttccgggc ataaaacttt acgaatatct
     6421 gattctaaca aatgtagtac aataaaaaat tggggaacaa tttgcaagag ttttcgtcaa
     6481 aataatttat caacaactga ataaagccaa ttgggtatga tgtaatgaga ttatgattta
     6541 attacaaaat tttcaattgc gatggcaaaa atcaatttca ataactttct gctatttaca
     6601 cacgaagaga atcacacgta tacagcataa acactcataa tacaggtaag gcgggtgatg
     6661 cggaaagttg acgctattat ttatgtattt ctgctattat ttttgagtgt gtttcaccgc
     6721 tgtgttcata cagcaaaagc attgctatta ctatgtaatt ttcaattagc ttctcttgaa
     6781 ttatcaccaa agtatcattt aaatatcatt caaatattac ctaaatattg tccaaatgtt
     6841 gttcaaatat aattcaggtg tcgcccagga ctttcctgag tttcgctttt cattaatgat
     6901 aacagcaaac aacggtttaa acatgcctgt tccttggtcc ggcaaatttg ccggctccag
     6961 aggccaagga tgggttgggt cagtttagtc cgaaatatcc aacaattatt actttttttt
     7021 aaaaatcgct tcccggaatg ttaaccaaga cataattcat gtttaagtcc tttaagacct
     7081 taatgtgacc cctagtgcaa tgtcagttga gtttattaga atgtcaagtt tatggtgcag
     7141 ataacttgac attttgcttt aaccctgaca actccctgta gcggtaaact tgcatctact
     7201 ctatgataaa ccagttctgc ggcaatttct gggattaaag gctatgatga ctcctttctt
     7261 gcaattcgat cccacggttt aggtatgatt aaaatttgta taccaatttt caagtcctga
     7321 tttccatcga gaatctggat taaatcaatc accagcaccg gtttgttgtc tgattgattt
     7381 caatatcttc aggatgagtc ccgattcaaa agcggaaacc agagcaccgt cgcaaaatct
     7441 attgcttcgg acaattgcag tgatgatttt acattttgtg ggtatatcaa ttgaattaaa
     7501 atcaaaattt tcatctcgta ccattttcct accttcaccc caggaacccc aatcgaaatc
     7561 aataataatt ggcatgtcgt acacaattct atgaaagcgg gaaaccacgg gtgactcaat
     7621 ccagtagggc ataaccatga tgccttcagc gtttttctct ccgcctttca tttctccgaa
     7681 tgttgaagtt tgttcaattt cgggaatcag atccaatatt ggtttccaat cgcttgagtt
     7741 aaaaccattt atcgtttcaa tgtaattgtt ttcgtttaaa tgcatgcttg tgtttttaag
     7801 ttttgacatt aacctgattg tttttgcatc atattgtttg cacaaattat tcaaagaaat
     7861 cgggattgtg actcattccc atattgctca tcgccctgga gaaatgtttc acataattga
     7921 tgccctcttc ggggtaatcg aatatatacg cgctgttctc gtatcgtatc aattgatttt
     7981 tgtgcttgtt cagcacctca aacagtgaag caaattgctc ttccgaaatg tgcagtctct
     8041 cggctatttc ggtttgtgtc aagttgagta tcccggatgt gggaaccaat ttaataaggt
     8101 ttagggcatc aaaatgaaat ggctcgtcaa tctgtgcttt aatgatgtag tttgcataat
     8161 ttgatgaaaa catagacgtg ttatttagtt ggttttattg ttttgtattg tccacgaaat
     8221 acaattcagg gcaccgcctt cgtccaaaat cgggagcatg ccgggaacaa aaacgatact
     8281 gttggtagcc gcataccggc tgtaatagtc cgaaagttgc cggaatgcct gttcatcttc
     8341 gtcggcaccc agttgcggga ccagaatcag gtcgcgggtt tgtaagaaat tgaggtatgc
     8401 ccaattgtgc cggtgttccc tggccacatc aaagtgtaat ttctcaattc taagaccggc
     8461 aagctgcaac ggtttcaaca gccttgatgt gttcctatag atgtggttca ccaacaccgt
     8521 gtcgtcatca ataaaccgga catatccatc ggtatgcccg cattcatcag tttgtttttc
     8581 gagccaggga acaaacacta tcttttcggt ttccaacaat gcttcaagtt cgtcaatcac
     8641 tgcttttttc gagaaattgt gtcggttttc ggcaaaaact ttctccgaca ttattacagc
     8701 cttgcttgat tttaccacgt taccgccatc aacgattaac cccgatttca acgtgggtat
     8761 agaatggtgt tggcacacga tatcagggta ggttttgtaa ttacgccggc atttcgccgt
     8821 accctgcagg tagtcggggt catagcggaa ctctatgaat ttttcatcag aaatctgaac
     8881 cggcatatag tctcttgccc agatatcgtt tgtgccttgc aggaattcat atgatatatg
     8941 gcgcgcagtc agttcatttt caataaatct gcataccggt ttataggatt gcaactttct
     9001 cagccattcc gagaagtaaa tggtttcggt ctgccaatca gctatcatca ggtcagcgta
     9061 ttaatgtttt gaataattca aacatggcaa atagttttat ttgtttcatg gaataataat
     9121 aaaataaata aatattattt atatatataa taatataaac tgttaatatt tgcaaatata
     9181 cacatgattt taatccggac aatagttttg tgaaataatt ttctgtttac agtgaaagtt
     9241 atttcaaaca caaaatgtta aacttaggtt aaagtcaaaa agctgtttat attttcgtga
     9301 aataaaggta atatatataa atattctttc ccccattttt gcgtttaaaa ttctaattca
     9361 aacctgattt gaaagctaaa tatttgctgt aattgcaagg attagagtgg gggtggaggt
     9421 tctcttattt tggcagtaca acttccgatt tctaacccga ctttcggagg gaaatttcat
     9481 tttcacgcgt ttttagtgac ccattcatca aattcttcca ttttaaaact caaaaattta
     9541 tgaaaaacac gattttttac gtgctcaggg gcgaattcta tcgtacccaa ctcgacacca
     9601 ataatctggt gaaaatttac aaaacattcg caagcaacaa accgcttcat gcgcgttttg
     9661 cagcgtttca atcatttcac gattacatcg agttgctgta cctggctaaa aacaagcgct
     9721 acagcaatta caagcaggcc gttgtcgatt tgcaggactt ttttaactcg tacagcctcg
     9781 atgatattct taccggaaac gactccgata cttttcgcgg catcagcctc agcatggtaa
     9841 tagatccccg ggtggaatat gtttcatcgg aacggattac gtattacaac gacgaaatcg
     9901 agatacacgg cttcagcaaa aacagcaacg acttctccat tcagcgtgag tacttcaata
     9961 acttgcgccg tgaagctgag atttacaagc aaagaggtat tccaaccgga gtcaatactg
    10021 caaaatacga catctcaccg cttttcgaag accccgaaat catcgaaata ctgcccacac
    10081 ccatcaactt caacgacatg atttcggacc tgctgatcat gggggagagg gtgtagggag
    10141 gtgaagctta attcttattg cttcgaaagt cgtcaaggta tttctgtgct gtttcgagaa
    10201 tgtgagggtc aagtttcttg ctgttaatta ccaggagcag ggcttcttcg gcaagccctt
    10261 cccgaataat tgatttggca gaagagattc gggtgtgcgg ggttttgaac tctcttattt
    10321 tttcgtactt tttttcaaag gtttcgagcg aggtgttttc atctttcgaa agttcggggt
    10381 aaaatatttc gataaatgcg gtcttgccaa tgcttttcaa ccaatgatca agtttttcga
    10441 cacgcgtttc ttcaccgatt tctttggttt ttattttagg tgtttttgca attccggctt
    10501 tcgccgattt cttggattgc tgctttttct ttttcggttt tacaacgctt ttgtcgaacc
    10561 attcaatcaa acccagtgta tgctgaattt cgggggcttg atacgccaaa cttatggttt
    10621 caaactcctc gtattctttt ttgaattcat tgcgcaggta attccttaca aattcgagca
    10681 attcggtttc ttcaccgagt cgcaaaccat cgtaaatcac attgttgttt ttgggaacca
    10741 ataccgaatt caatggatga attaatctga caaaatgggc agtaagaagt gttctctgat
    10801 cagcactcag gttttcgttg attctcctga ttttgtcgtt tccccattcg gtacggttcg
    10861 gattcgggaa atagctcatc aggttttccc cgaaatctcc caaaccaaat ccggtgggtt
    10921 ttatatgtgc ctgataccat cccaatgcat tgaacggaat gttcagtgct ccgctggggt
    10981 tgtaccaggc cagctctttt tcgtttgaag tgagtccgaa cgaaactatg attgcctttt
    11041 tattcaggta gtctgccaga tcgttgcggg tatattcaat gccggcaatt ttcaacccgg
    11101 tgaaatacat cgagaatgtg ttatcgcaga acaccagttt tgttccatcg cgatactctg
    11161 ttacaaagcc ccgtcgcaac aaatcatagt ttttagccga gccatacaag cgcaaaaaca
    11221 aggtgggctt gtccgatttc acgtattcca ttaacgcgtt gtgcagtttt acaatagctg
    11281 cttttttcgc aaattgctgc ctgataattt tctcgaaaaa gtcttcgatg ttttttgcac
    11341 tgcccgattc aaggcgcggg aaagggtaat ttctaagatc tgtcatagca aactaaataa
    11401 agttgagtga taattacatt ttgagttatt caacaaaaaa atatactttt tactagcact
    11461 tcaaagaaat ccaatttagt tttcagtttt tttattgtag atcatttaga tttgggtatt
    11521 tcttcaccca acgtaaataa tgctgtttga aaggcaaata tgttttttgc ttttattttt
    11581 tgaccaaaca agtcgccggt tcctgcgatt gcttctgcca accaattcgc tttgatatta
    11641 ctgataaaat gcataattct attttgcgtt atttcgggaa atttaaaaga atcggtgctt
    11701 gtggaagggt tgcgctggtg gtttccgcgc ccaattccat agccaaatga aagttcggta
    11761 ggtaaaaggc tgattccctt tttgtcgaga taattcttta ttagataaca caatgcagag
    11821 ccaaccctgc catcatacat aatatagttc tcatcagatg ctgcatatat tttggtgaat
    11881 cctgagttta tataattcgg gtttaaatca tctatgatta ttttatcatg gttcactaaa
    11941 ttcagaacag cttgaataaa ctcataaagg gaatccctga cagcaatttt atcttggttt
    12001 tttgcagcca caccacccca gtttaaaacg tcattgcaat tttttctaat aattgactca
    12061 ttttgacttt taaaatcatc acgatatttt gcgaaacgat tgtaggttct atcaaaatct
    12121 tccttaactg atttacccca ataataatta tttacagctt ctgtaaaaca gtggtatgga
    12181 tagttcaaat cagtgagtga tttctcacca ttaatatagg gttttatcca gctaacgaaa
    12241 tcttgagtaa caggtgactc aaggaacaag ggtttcgata agatgcctct gtcaaactct
    12301 gatgtaaatg tgttttccat tttaaatttc tttatgaatt gctttttaat tgatatccca
    12361 gcatctcttt cgccagttct aaatcgattg tacctttttg aagtaatcct tttactacat
    12421 cttctttttg tctaaacttg tgagcctctt ttaattctac aatataacga tacggtcttt
    12481 cccaataatt atcctcgtca tccgattttt ttgtaagtcc cattctggtt gatttgaccg
    12541 gttcatactt tggaatgtcg tattcatttt cgttctcaac aaaataaaaa ctgggtctgc
    12601 catcactgat gtgttgaatg gaagagaaca aaactttcag acaatttgaa acacctgtga
    12661 tatactgatt ttcgccatca agttttcgta ttcctcggat taaaatccca ccatagatgt
    12721 tttcatctac ggttttagcc ttgcggccaa atgtaatatc aagacccatt ccgttaaagt
    12781 accaaccacc ttgtgtcatt tgctctggaa cgctatgagt atatggatcc ggatgtttaa
    12841 tacttttgta atatacctct gcttccacaa tccggtatct ggtttctcca actacaagac
    12901 acaaacggtt cataacgtag tccatcaatt tatcaaaatg attatagttt ttatcagcat
    12961 aatcggcatt gtaaattgtt tcgcaaaacg actggaattt ttgggtaatg ggagaggagg
    13021 tggtattcat atcatggggt taataggatg atattccata tttctcttcg aggaaatctt
    13081 ttagatcttt accaactaca tgggcaacat cttgccaata gataatctca cagtttgttc
    13141 taagatcgat atacctgtct tgaatacaat tgactgtatg aaaaaaactc tgagcaagat
    13201 cggggcggcg catatcgcaa aacaatataa accgtccacc ttcaatttgt ctggcagcaa
    13261 gtatgtttct gataagctgg tagttcttgt attctgtttg cgtttgcaac aatttgtctt
    13321 tatgaaatac ttgttcaaag ctcaaatatc tttccacatc agttttatta tgtgaggtga
    13381 aatctttttc agttaatttg cattcacata taattgaatt attgattctt atgtctattt
    13441 cggtagtatc gctaatgata actccatttt ttatcttctt aacattgcca tttaccccaa
    13501 attctatagc agataatgaa tttactttta acaatttgca aacacctgcc catgttttaa
    13561 ttattggatg gcaaaaaatg ttcatcgtca gcgcatcaga gctgttcgat gattgcatct
    13621 cataaattgc agggcatgga agacttttta aatgtgaatg aattttcttc gtcctatcta
    13681 aataggatga gtttccaaat atttctttcc aactctcctt atgaaaattg taagaaacgt
    13741 cattaaatat gactgcatca gtcgacttta tgagggtgtg tgcatatgct tgcccatagt
    13801 ttttgcttcc atatttatgc gctaatgctg ttaattcttt tcttagtaat tggttgtaca
    13861 tggctttgtt tgggtttggg tttgggttat tctatcatta aatttctaaa cttttcttga
    13921 aactcttttg tctgaattat gctgaatact tacaaatcgg tattcttcga tttattattt
    13981 ttcatccata tccacagcca aaccgaaatt aaatcggcaa ttgcaccggc aaatatttgt
    14041 cgggtttcgg cttttactct gttcattgat tcattgcccc attcttcggg tttgtcaaat
    14101 gggaatagca tgagcgattt ttctttggtg ctcacgcatt tttttagcat gaattccatc
    14161 aaatcgtttt cggcaatcag ttcgggttta atcagaccaa tgtgagcagg attgaaaata
    14221 tcgaaaataa tggcttccga aagttcggtt tttacgggtt tatcactggt gcgcgatggt
    14281 tttattattt cttcgcttac tgccaccggt gtaaccgcat catcccacaa ttgttctaca
    14341 ttcccgtgaa ggctttcttt gaaataattt ccattcaggg gttttgttgt atcgcttgct
    14401 tctggcgggt catcacgtag gtcgcaatgc atcggaacgt tggcatccac aatataatga
    14461 gagatgagga ggtactgata cataatctga ttcatcgaga acctgtcgtt gtacaaacgc
    14521 agttttatca tatcggatat aacccgggca aggtggtcaa ccttataggg taagccacca
    14581 ccgccacttg cacgatagta tttttcgaaa tgcacttctt tggcaaagct tcggctaccc
    14641 gggtattcct gatcggtaaa caatttaaat gtatgataaa tggctttgtc gttaataata
    14701 tcatcgggag cccaggtggc ttcatcaata aactcgatgt aatgcatcag catgcgctga
    14761 agttctttcg cggtaatgcg caatgccttt ttccttttct ctataatggc gttgccggtt
    14821 ttggagtcga taatatttga agacccctca tacaggaact caatggcttt atatgccaaa
    14881 tagatgtggg tgttgtgttt catggttttg gtactaatat ctttatttac aaatttttct
    14941 aattaaaact atttaacgaa tttcaattca acacgtctgt tcttcgctct gcctgtatcg
    15001 gtgtcgtttg ttgcaatcgg ctttgattcc ccataaccta tggtttgcag catggaagga
    15061 tcacatcctt cattgattaa gtaatctctc actgcttcag ctcgtttttc tgaaagtttc
    15121 tgattgtttt cttcttcacc aatattgtcg gtatgaccac taatttcaac tttcaaacca
    15181 ttctttttaa ttatccctgc aacacgctgt aattctggca gggaataggg gagtagagag
    15241 ctttcggcaa aattaaaaaa caggttattt accggcacag cagtgccttc ttcaatcatt
    15301 tgtttaaaag aaactaaatc tatgttttct tcgattacaa ctggttcagt actagttctt
    15361 agatctatat tatttgatat agggaaatac tcgtccccgt caacataata gccataaatt
    15421 tttcctaaag ggagtacaat aaaaaagctc ccatcaaccg ggtcgctttt cgactgacct
    15481 acgttttttc ctgttgtcaa gtcttcccat cttatatcgg ccgatacagg ttggttgtca
    15541 gcatcaacaa gtcgaccgga aactgtggca accatttcgg gcctgagatg atttggaagc
    15601 ttaatccagt aaatatcatt ttttttgcta ttttgatctt ttttggaaaa atatgctttg
    15661 ctgccatctg ttgatacttt gtaaccccaa tcactttcag ctgtattaat ctctttgccc
    15721 atattaattg gatcgctcca acagtcccaa catgtatccg aaagtcttgt tgtcttataa
    15781 acatcatatt tacctaagcc accaagtcca tcggaactaa agtacaatgt tttcatatca
    15841 ggatgtagga atgggcttct ttctgaatac accgtattaa ttatatgacc caaatttata
    15901 ggttctcccc aagaattccc attccgaatg cttatataga tatcagatgc gtaattatta
    15961 tcgcaatgat atccgcttaa tccaataggg gtgtagttaa atcctccatt tctggctgat
    16021 gcaaatatta atgcatttcc atcactggtc aacattgcat caccattcca atctcccgta
    16081 ttaacattgt cagggaaata ttcgatttca gaccagccca cttctgtctt ttcagaataa
    16141 cctagcttac catctttaaa ttgaaccatt ttattaccat cactacttaa tgccattggg
    16201 gcatcatttg aatttctgaa tgataaaaaa cgatccaact ctggtttatc ccaattattg
    16261 ccttttcttt tggaaataaa aatatcttca cctccaatat tatcctccct atcttttcca
    16321 caaaaataca agctagaatt atctgcagat atcactggag agtattctcc cccattcgtt
    16381 gtattgacca atggtattga attaatttga attgtattat cactattact ctgcagaata
    16441 ctaagcagat cagagtattt tgaatcattc gtaaaataat tttcaaattg ctttaccgtg
    16501 gtaatagcct cattccattt tttgtatttt atactattag atattaatat ttgaagagca
    16561 acaaatgcta aatcatttgg agcagccata ataatatatt gctttaactt agaaatatta
    16621 tcttggttta attctatctc accaaaaacc tcaccttcat aattaaatga atgtatagtc
    16681 ctattgggtt gtgaagcact gatactacca gagaattctt taatactgtt tccaacttta
    16741 agtcttatct tatatttcgt tggatctttg caatagttgc cacatctatg atttttaaaa
    16801 tgatctaaaa ttatagtata ttgccctaaa ggtgcttttc cattttccca atagatattc
    16861 tcaaccgggc ttaacgaatt agggctaaca ttcatatcaa cgtctaattc acctccactt
    16921 attgattttt tatgattata gtaaatttct tcaccaaaag gatcaataca atgcaagtca
    16981 agatcattgt aattatccca catcaacgaa atttgaattg aaccagtttt tgccccttct
    17041 cgtattaacc gttgattcat ttcatcacta ttattgtaat atgagtcatt tacagatgat
    17101 gtgattccaa tgttaaaagc ataccttgca ttttgtaaat caatatcaaa ggaatcaatt
    17161 aaattcatat agccagagaa ctcatattca aatctttcaa gagaagagag ttccccatct
    17221 tccgataata aatcgtagat agaaattacc cctttattat attgctctcc tttactttta
    17281 gaaaggccgt atgcaattaa tggcaaaaac tttttctcgc ttccaatcaa atataggctg
    17341 tctattgcat ttttaacata acgattatct ggattgtttt caataaacca gacataacta
    17401 tactcattac cggagtcagt ttcttcctca aataatctag tctcaaaaag aaattgagca
    17461 tcattaattt gtttgctatc cggatatttt tttatgaact ccttatatgc attcgagtta
    17521 tcaattcttt ttgcttcttc aaaggctaac ttgtctctgt tttttatcgc atctgggatc
    17581 tctgaagcgt taggataatt attaataaac ctttgaaatg acgataccgt gttttccttt
    17641 tctgcatttt tgtacgcaag ccgattccta ttcttttcag catccctaaa ttgtttcgca
    17701 acaggataat tattcatgaa tgattgaaat gattcaaccg tattgatttt agatgcttcg
    17761 gcatacgcta cgacatccct tcgctcgata gcggaatttt tgtatttctt tggcgataat
    17821 tcgaaataac ttaaaaacct ttcatatttt acaatcgtat ttattgctgt tgcatcttcc
    17881 aaggcacatc gcgatacagt gtcgagcaga ctttcaaact ttgatttgtt tattggaatt
    17941 ttttctaatt gtttaataat cttctcgtcg tttacttgat tgaatttacg ataagctttt
    18001 gtaagatatt cataagattg ttccgggttg tatttgttaa attctctcga aatgtagagg
    18061 taagccatgg tataattcaa ttcgatgtca tcagaagatt tttcaatagc tttgtttaat
    18121 ttcttttcgg ctttcgcatg tttcccatca tcaatcattt tcatatagtt gttttgccct
    18181 aaaacggcac tatatactcc tatcaggatg aaagatgtaa ttataacttt ccacatagcc
    18241 aaatcagtgt tatgataaat atattatgaa aatagacaaa taggtgcgtt caaaaacact
    18301 tacaaatata gtgtttcaac ttatttaaaa aaacaaagta aatttctttt tggttaatag
    18361 gtagcttaaa aactttttct ttcacattga ttaatattgt tttcctcccc aaaacttcgg
    18421 caaaatccaa tctgttttgc cgaagttttg agttggtaaa ttatcacttt atcaaattat
    18481 cagtttatca aattatcagt ttatcacttt atcattttga aaatatcgat tgtaaaaaat
    18541 gcttcaacgt cgtattcatg aatttatgca acagcctgtt gcacaattta aatattgttt
    18601 ttgccaacac actggtagca taggaatgaa gccttatgac gcttatgctt cattgtatta
    18661 atttcgaaat aaatgtcagg taactaccgg tagttacctg acattttata tcttttttca
    18721 aatacggcaa acatgattaa ggcatgatat tactgaattg cagattaatt gaccactctt
    18781 aaataatatt ttcacctcat aaaattcatg ttgccgttgt tttaatatgc ttagaccaaa
    18841 agtttatcac tttatcactt tatcacttta tcattttgat cacccgactt ggtgtaattt
    18901 ccataagatg gttttgtgaa aatgttactg ctgagaaacc ggtcgcagct ggcaggggag
    18961 aggtcgagct tttcggcaag ctgcagggct tgcttccggg tgaagctgtc ggggagcaaa
    19021 tcgtagtact ggttcagtcg ccgttgtttt acgatatttt tattaccggt tcttgtttcg
    19081 gcaataagcg tgatggcatg atggatcagc gcaacaacga tttcgtatgc aattccgaaa
    19141 tccaaatgtt caatggtcat ttggggttca ggtttcttac cactttccaa aacgcgcagg
    19201 gtgcaggtaa tcatcgcaat acgaaacaga atcaagccaa gtcgcttcac cgtggccagg
    19261 cactgatctc caatctcttt gtgtagctgt tgcataatgt cgttcatgca cgatttgaac
    19321 tctgccctag ttgtcgtatt gaattgccat tcaatggtgc ttcccagcga tataagcctg
    19381 tttctgtact caaacatttg ttgtcccagt tcattgaata ttcgcaaggt gcgatgctcc
    19441 atatcgataa atacatcttt aaaaaccgta acggcaggga aggagtagaa cgcaaaccgg
    19501 ctgaacaaac cgttctcggt gctgggaatg agtttaaaca actgatcttt ggtgcctgtc
    19561 agcaatgccg aaagcctggg ttccgaaagc tcaaggtatt cacggttggt tcttcgttgc
    19621 aggctgatgg gttcatgctg aaatgcacaa cgcaacacgt cggaatagtt tccccagtcc
    19681 tgcgataagg aattcgccag cgtgtcggct tcggtgcaga atagcaagcc ggcaccatcg
    19741 ttttccgaca aggtctgcag aaacgaactg gcactgttgt ttgccggaat aatcaacatt
    19801 tttctggcgg gttcaccggg aatttctttg agaggttctt ttgttttctt cttcaacgaa
    19861 ttgcgataca gcctgagctc cgactggtag gcttcgtagg cctttgccga agtgttgagc
    19921 atgtctttgt gaatgaattc tgcaaatttt cgggtccatt gcagtattcc ttttccagca
    19981 ccggccggag cctcaataaa cacaaagaaa ttcgggtaca ccgtcttcat gtcgtacatg
    20041 ccacacacaa agggcaggca tcccgaaatt actgacaagg ccccgagtag aataatgtcc
    20101 ttctctttgg gattcgagaa ctgactgcaa gcatccgata gtgcttttgg cagatacctg
    20161 tacacgtcgg gatgaaattc tgacgggaaa tttttcactg actgaggaat gataaaatct
    20221 tcttcctgtt caggcaatcg gtaggtattc atttctttaa acatggcttg ccctttcatc
    20281 gcttgaatgg ttctgacttt ttgcattttc gagccattga atcaagtctt gcttactgaa
    20341 gtaaagcttt tttccctgct taatcgaagg gatttgcttc ttcgagctta acccgtagag
    20401 tgttgtcagc gaaattctca gaaacgaggc tgcttcgttg gcattcaggt aggcatcgag
    20461 gggatgctct gtgttaggat tcggctgcgt ggctagtctc tcgtccagca gcgtttcgat
    20521 tacattgcgg attaccgctt gtaaactttt tttagtcgta attataattt tcatgacaaa
    20581 taaaatttat aagcgtgcca caaataaaaa gcccaatttt atgccatgtt tccaattgct
    20641 ttactttgct tcgatgtctt gataactttt tttctttttg attcttttgc tttttcttgc
    20701 tttgttttgc tccgatattt atcaactgcc aaatgtacat tctgacattt tgttaataac
    20761 ttaaatcatc cttacctcag cctaataagc tgatgaattc gtgttagcac actgttagtc
    20821 aaacgatttt taaccttctg taaatcaagc gcagcaaggc ttgtagaaag aggcaaaatg
    20881 cttcccaagc tgagggtcgc gggttcgacc cccgtttccc gctcgaaagc agaaagagtc
    20941 tctggatgaa ttattcaggg gctttttgct tacttcaaaa ccacccatat ttacctcgac
    21001 agcttcgacg acgaagtaat tgacaatgca aataagttag tgacagggta gggggttatt
    21061 tcagcaaatc tagatacatc agtttccttc ccagtttgtc gtcgttagct atttttaaaa
    21121 tgtcgttttc gaggcataac gctcgtaata cttaaatgcg gcatccagcg aaacacccgg
    21181 actacctttc tcgatgagat acaaggtgtt gtggaaccgg atggcaaata aattcaagta
    21241 gcaacattag gcacatcaag aaatttaatt tataaaaaat atatttatgt ataattcaaa
    21301 tattatacat atttttacta caactaaaaa cagaataatg ctgaaattga atcataagta
    21361 tgtaaagcat tgtctttgtg aaagtaataa aatattttgc ctgctatgaa ctatataaag
    21421 ctactgttaa aattctgcct atttttaggc gtaaccacaa atagtagctt gaaattgttt
    21481 tcccaagagc ttagcacaat aaaagaaaac aaacccatca gttttacagg cgctctcaac
    21541 acatcgggaa cggtttattt taccagcgac gataatccac agcgagatcc ttttatatgg
    21601 aatgtatcgg gaaacttcaa ccttaatatc tacgaagtat tcgacattcc attttcgttt
    21661 gtgctgtcga aagaaaacaa aactttcaag caaccatcct atcaacaata tggaattagc
    21721 ccaagctaca aaagtgtaac acttcatttg ggatatcgta atttgaattt ttcgaaatac
    21781 accatgtcgg gtattacgtt tttaggggca ggcatagaat tttctccaga aaaatcgcta
    21841 tttaaatgta aggcctttta tggacgtttc gcacgtgcac aacaatttat tcccgacaat
    21901 atgttcaatc cccaagccac gtttgtaatg ccctcgtatg agcgttgggg ctatggaaca
    21961 atgattactt tgggtaaaaa agaccatcta ttcgacattg ttttattcaa agcttccgac
    22021 gaccctgcct cgtccgctat tcccgacagc ctctcggttt atccggccga aaactttgtt
    22081 gtgggactca attcaaaaaa caatattctt aaaaaccttt ttcttgaagt caattatgca
    22141 ttgagtgcat tttcgactga tatccgtcag ggcgaaatat accaagaaac ctatacctat
    22201 tcgaacaatc tgggcttttt gtttactccc cgttcttcgt cacaattcaa cagcgtgata
    22261 gatgtagcca taaattttaa acccggaaaa gtaaatattg gagcaaaata cctgcgaatt
    22321 gatcctgaat acaaatcgtt ggggactgct tttatcaaca acgacgtgca agagtttacc
    22381 ggaaatattt caaccacttt caacgaaaac agaatttcgc tttcggccaa tgccggtttg
    22441 caaaacaaca acctcgacaa atcgcaactt gccaccaaca aacgatttat atcagcacta
    22501 aattatactt ggctaatttc taaaaatctc aatttaacgg ccatcttctc aaattacagc
    22561 accaactcta tgcctgtgca ggtgtacatg atagattcgg taaaatactc acaggtaact
    22621 cgcaatgcct ctttgaatat tacagaaact tttggcgaca ctattattca acacagcctt
    22681 tcgggaatgt tcagttttca gggaggcaac acgctcaatc aaacaggtga tgctgttagc
    22741 gacgtaagta atacctttgc taatgcctat ttggcttacc gattaaacta cctacccata
    22801 gggtttagcg tggtggcatc gttgaattat tcgaacttca tttcgggcga cattaatacc
    22861 aaatcgatag gtcccagtgt tggggtaaac aaatttttcc tgaaaagaaa aataaacacc
    22921 ggaataaatg caacctatat gaattcgttt ggaacaaatg cgaatagtaa ccaaattatt
    22981 aatttacgct tgtttgccaa ctacaaaata aacaaatacc acagttttac gctatcggtt
    23041 aataacctat ataaaatagc ttcaggagca tctgaccatc attctcaagc gagcattgcc
    23101 tataacctga ttttttaaaa attaagccaa tctatgaaga aaacattaat actcgtcttt
    23161 atactgttaa gcatagccgg tttttcacaa ataaccaatc cggttacagt taacttgctt
    23221 cttacgcctc cgcatagcgc catgctaaac gaatatacca cgcccggctc cgataaaatt
    23281 attgcaaata ttcgcttaaa cgaccttaac gaacccatgt ggaatgtacg cctgaagata
    23341 agtctcgaaa gcaatatggt gcgtatttcg acaaaagcca actacaggcc accaacgccc
    23401 gttgagttgc tgccggggtc ggtaatacag gtttcgggcg aagaccttgc tccataccta
    23461 aactacgcca acatcgatgt gcaaggtatt accatgtatg agttgcaaca aaatggaata
    23521 ttacctgagg gcttttacac tttcagtatc gaagtgtacg attacaattc gaatcgtttg
    23581 ctgtcgaaca ctgccaattt caatgcattc attcagctta acggggttcc ccttattcaa
    23641 acaccggtta ccggaagcgt agtgatgcac tctagcccgc tatacattcc tttccaatgg
    23701 caactatcgt ctccgtcaat ttatggagac ccctcaacta ttgaatatca gctaagcttg
    23761 tatagaatta ttgaccccaa tgtgaatccg gtaaattcga ttatcaacaa tgtggtcgaa
    23821 aaagtctatg aatcaaactt ttcgaatgca accagtttag tttatggaat caatgagcca
    23881 cctctcgaag ttggtgaaaa atattctttc acagtgcaag cacaagatgc cgaaggacga
    23941 gatatcttta aaaacaaggg tttttcggaa gtaggttggt tctattacgg ttatcccgat
    24001 ggcggacata tcagcattgt ggctcccgaa aacggaaaaa acttcacact tagtgagcat
    24061 cgtcattttg aatggggtgc ccctgataat gttttatcag atcagcaagt atattattat
    24121 ttgaaaattg tggaattggc cgaaaatcag gactcaatcg atgccataaa taacaatgtg
    24181 gcttggacag aattcacaag tattccccaa ataggctcca atggctttgc ttacacactt
    24241 accaaagcac ccgaaaccca acaagaatat gtttggcaag tcaaggcatt tacaggcgat
    24301 caggaaattg catcaagcga tgtgtacaag tttattggtc caccggtaat tgaacgtttt
    24361 atggcaggta gccatgaagt aattgttagc caaacctata atcaagattt gtcgaacctt
    24421 tctggaaagg gcaaggttaa aatatcggaa gcaggaaacg aggtagaatt cgagttcgaa
    24481 ggcttgcaaa tatcaaatgc aggggggcgt agcgtgttgt cgggaggaga attactcgcc
    24541 gagctcgaaa atttcgataa cattcaatta tcagccaacg acaccgacaa tggaactgct
    24601 gttttttatg ctgactctat caagctaaac aaagaagaac tagccattaa aggacatttt
    24661 gtgtggccct ttgttttagt taccgatgcc cctgatttgg cgaatattgt atcaagtagt
    24721 gcatgggtaa attatgatgt ttataaattg cttggaagca tttcgcttgg gaataacaac
    24781 agttttactt taatcgatcc tattgggtat gagatttcgt tcgatagcac tgccaacttc
    24841 ttgatttcag agaataggtt tacaccccgc ttttatggca aattaaccct gcccaccagt
    24901 gtaaaaaaca ccaacaacga aagaatggtt ttaccctttc gaaatgtaaa caacctgttc
    24961 tatttcaatc aggaaaatgt tcaggcagag cataaaatta aaatcgccga aaatgcaaac
    25021 cttgcattta tacctttggc atacacagtc gattttagca atgtcgatgc tattgatcaa
    25081 ataggcaaag atgcaatgtg gaaaggtgtt ggtttcgaaa aagcaaaaat tgaaattccc
    25141 gtgaacgtcg atatgcaaca acgcataaat ccggaatatc cgatgcaata tgtattcgat
    25201 attgcaggca cacctaatca atttgcctat atcgacaacg gcggtatgac tgcaagttta
    25261 gaaacaaact tcagtttata caacgcgggg aactattgtg ggtttgctat aaatatcacc
    25321 aaacttaagc ttcttatcag cggaaacgaa cataccaact cggaactatc tgtcgctaca
    25381 aaaatcccac ttttaggaaa cgacgaaatg gctgcaaaca taattattga tgccaatgga
    25441 tttcagatta tggatttaaa tacccaactg ctaaacaagc aaattgtttt aaacgctgat
    25501 aaagaaaaac tgaaactcat tctaactatt aaacaggctg tttttaaaga caatgaaaag
    25561 ctcgagttca cggtcaatat ggattggcct tatatgcaaa cctatattcc gaatttaagc
    25621 ggcttatgtg tttggggaaa tgacgatttt ggattcaatg agccgaatgg cgcatgcatt
    25681 ttgagcaatc ctgtgtcggc taaatattac aatacttacg atttaatagt cgattcgatt
    25741 gtgtcggggc ggcatgggca aaattacatg atgggtgtga acggggaaat tgtgttggct
    25801 gataatattg caggagccaa caacaaacct ccacgtgtta ccatggcttc gtacgagcta
    25861 aaagaaggag ccagctctac ttacgatttg tcggtagaaa cttgggtgca gagctttatt
    25921 aacaaggcgg aatttctgtc aacccccgaa aatatctttg cgaatttgcc ttatatacga
    25981 gtaaacacac ctgtaattga atttgaagga ggacttgttg ccacaaacga agaccccgaa
    26041 tggggaactg cattctacgc tttattagac ggaaccatca aacaacctca ggaatatcat
    26101 gctaaagtgc agtttttaat cggaaaactt gatggagagc cgtattggtt tgccgaattg
    26161 ggcttgtcaa ctagcggtca tgaacacgat caggctcagg tgcctgaagg gaaaaagaaa
    26221 aagctaaaaa aagttcaaat gtcgaagtct ggaataaaaa tcggacccct cgaaatcaat
    26281 gggattacag gcagggttta ttaccacatg cgacccgaaa cagccgtagg agttgactgc
    26341 aatatgaaat tcgatgaaat tgccgaaccg ggtctatcga actatcatct gggattaccc
    26401 gatttacccg atgccgatgt gtgtgaaatt cttaatcatt tgaacgcagc acaaatgaaa
    26461 tcattgctat gtagtttaca ttcatcagct tttaatgccg tattagaaaa atttccggag
    26521 cccaatttcg acgacattca gtcgtatata gaatcgcatg ggtcctctga ttcggaattt
    26581 ttgaaccaaa tgattgccgc cgaagcacgg ggcagaatgc aagatgctta tgaaaacggg
    26641 ggcatacaag atcaatatca caagtttaca tatgacagac ttgccaaagt ttttccgggt
    26701 tacgactggt gccaaaaagt gattgacgac aacgatgaag gcatgcaatg gggtaactta
    26761 ctatgccaaa tgcccaacat acaatggccg cgattccccg atgtttgtga actatcagag
    26821 tatttcttca atagagtact cgaagaactt ccacctcctt catacgatga tttaaacgca
    26881 attatgtacg acgaaaattg ggacatgatt caagtagatc atcccaacat aacatggccc
    26941 gatattcacg aaatctttcc cgaaaaaaat ctttgcaaat ttatcatgat atggcccggt
    27001 atcaattggg gaaatatcta tgtgaatatt cccgaattac caaagctcga atggcctgat
    27061 tgggaatatt attttcctgg attacccgac ttagaagtag ttctgccaga actgaacatc
    27121 gaagtaccct caatctcatt tgaaccaggc gaaatagaag ttgattacaa agtggacccc
    27181 tcagtttcgt atggagtgta tgtattggta aattatgtcg atttcgcaaa taaaggcgag
    27241 gtggttgaag gcacaggcac catggaagta aaattcaccg acgatggcgg gttagaagat
    27301 attggcttcg aagtgaccag taactggggc aattttgccg gtgctcctcc tgtaatcgaa
    27361 ggtttgggat gcatgcacta tagcagagat gccaaccggt ttgtaggcga cttttttggg
    27421 caggcacgca atccggcagt ttgtgggcac ggacacttgc atattgatat taatcctgat
    27481 ttttgggaaa taaatctggc ttctcgcgaa aaccctgttg ttgtacaacc tttatgttca
    27541 aacggacctg tgcgttttac cggatatttc ctgctcaatc agtctaggtt tgagataggg
    27601 ctgggtgctt cgatgggagc agaatttggc ggttcgatta gtaccgaggt gtgttctctt
    27661 ggcataaaag cggaaatatc gttaaaagcc gatatcttgg caagtattca gtataagcca
    27721 aacataaaaa tcaataaagc atattttggg gttgatttcc atgccggttt atatgtaacc
    27781 accagtggaa ccttatgtga ttttggcaat tttgaattgg ccagtatagg tttaaaaggt
    27841 caactcgaat attatgaaag atatctttcg ggtaaagtct ctgggcatgc aacatttctg
    27901 agcatcgtta cctgtgattt tgacctaagc accggaatta acttgtaaca cgtatgaaaa
    27961 acaaaacagc catagtatta atatttttgc tcatctttag ccatcaattg ctggcacaag
    28021 aaaacgccaa agtaatagtt agcaataccg acaactctga aatttgggtg aaatggtaca
    28081 gcgaattcat ttatttcgat tcccctgtgt ttttgtatcg tcaagaaaat ggaagaagta
    28141 catgggaatt tattggagaa tttagtcgtg gcaatggaat gtcgcgagaa atactacaag
    28201 aagacaaaat tctggaggtt ttacaaaact cggtaatggg aagcagagca ggcgatttgg
    28261 atggaatgac cttactaatt cttatgatta aatcggtaga acaccccgag ctgtgtcagt
    28321 ttttaggaat tcaacatatt gataaaactg taactgcagg taagcaatac cgatataaaa
    28381 tttgtaaagg caacagcccc gaagcgaatt cccttgcaat atcagattac attacttcaa
    28441 caaaatacga gaaagtcgac cccccggctg atatcaaagc atggcaagac aattatagtg
    28501 cgaagttttc gtggaggccc aatcccgatg catttatggg agtgaaaatg tatcgtgcaa
    28561 gcgataacga agacatggaa ctgataaccc ctgtgcccat aattcacaac ctcgacgaaa
    28621 atggaaatta tcaggaagca ttgtttaccg acgatagttt ggttctggga aaagaatacc
    28681 gctatattgt taaaagcatc gactactttg gcagagaaag caaaccgagc tccgaaatta
    28741 ttgtaaaaat aggtgatgtt tcaccacctt tacctcccca ccgactgggt gtaagcgtaa
    28801 ttggcaaaca ggtgcaagtg tattgggaaa acgaaaacat cagcgattta gcaggatatc
    28861 gcatttatcg agcacttgaa ggcaacactg attatattgc agtcaactca agtctgattc
    28921 ccaaagaaat tcaaacctat tacgatactg tttctacaat aggattatac aactataaag
    28981 ttgcaacggt tgatacagcc ggaaacgaag cactttcgat tgcctatttg gccgacataa
    29041 aagatgtttt tccgccagca gcaccacaaa accttaaagc cagttccgac accggaatta
    29101 ttcatttaag ttgggatatt tgtcctgaac ccgatctgat gggctattat atttatcgca
    29161 gtatgaatgg caatccacaa acctacatgc tgctcaatgc cgaccattat acgcaaaaca
    29221 atttctcaga taagttgcca aaacaaatat ccagtaattt ttcgtataaa gtagtagcag
    29281 ttgattccag ttacaacaaa agtgaatttt cgaatactgc atttacaaag ttgcctgatg
    29341 ttaccgctcc cgaaaaacca gtcattaaaa ccataacacg aaataacaat accttgatta
    29401 ttcaatggta ttcaaattac gaaaacgatt tgttgggata taacatttat cggtttaccg
    29461 aaaatcaaca tcccgaccag gccgaaaaaa tcaacctcga tgtaattact ggcattaatg
    29521 catttaccga cagatttgcc gagtacgaaa ccagctatcg ctatatagtg attgcagtcg
    29581 ataagcaagg aaatttctct gctccttcgg aggcatatac tgccccacga ttagcttatc
    29641 ctgatgcaaa cctcagtttc gacaaattca ggcttatcca taaaaacaac aaaaaacagg
    29701 tgatcatcga atggaaaata aacaagccag aggaaacaaa agcctatgtg gtttatcgta
    29761 aatcagaaag ccaaaaacat tttacacccc ttagcggaat gcttacagaa tcaagttatc
    29821 aggatatgat gaaaaacgaa aacggaacct atcaatatcg tgtgggagca ttgggaaaag
    29881 acgacaaaat gtattattca gaacctcagg aaattattat tctaaagaaa taacatcatg
    29941 aaaacactac tattaattgc aagtcttttg ttagcagtca atcttgcttt ttcgcaagtg
    30001 ggaatcaaca acagcagccc cgactcatcg gcagtgctcg atttaaaatc aaactccaac
    30061 aatcagggat tgctggtgcc acgacttagt tcggcacaga tagcagccat atctaatccg
    30121 gcaaccggtt tactggcata caacaccgat tggagtttat tctgctatta cgatggaagc
    30181 aactggtatt cgttgcgaac attcaggcaa attctcaatt ggggcgattt ggaaccaccg
    30241 caggatatgg atgccgatat ctacaatggc aaaaatatgg gcataggcta ttccggagct
    30301 aacagcaaaa tatcggttaa cggaaacatg agcattggtg ccaacgaaga agccccggct
    30361 aacggtgcct ttattttagg gcaagtgcgc atcggttcaa atgtaggctt taagaagctg
    30421 gaagtggatg gcgaaataca ttccactaac aacattgata ctggtggtaa aataaaagaa
    30481 ggcggaaacg atttactgcc cgccggttct ataatcatgt ggagcggttc taccccacct
    30541 gccggatgga aattgtgtga tggcaccaac ggcactcccg acttaagagg taggtttatt
    30601 atggggtatg gtgcgcataa cgaaaaaact cttaaatatg atggagattc aactcttaca
    30661 ccagtaaatc ctagtatagg acctaaaggt ggctacgaca aggttgcaat aagtgaaagt
    30721 caaatgccat ctcatggtca tagtgtatat ataactttta ccgatcgcta tacaaatttg
    30781 gaagaaagcg gacttagaaa cgatggaaca catgaaaacg gagataatag aactttcact
    30841 gtttggcttg acagaatctt taatgtaaca actggcaata acggtggaaa tgtcgcccac
    30901 gaaaaccgcc ccccttatta tgtacttgct tttataatga aagaataaaa cataaaacaa
    30961 tgcataccct gaataaaaac atcaaactaa ttttgagcat tgccatattg ctttcgtgca
    31021 aacttattgc ttacgctcaa aacacccaag acactattac aataatttcg aatagcgaaa
    31081 ttaccatatt gaacgatagt gtttttataa agggttgtgt gttcgataaa acaaactatt
    31141 cgtaccctta ccaaggtagc attttcaatt caggatatgt gtcgattacc ggcgatattg
    31201 taaataattc tggcatcagt ttggtttttt cgatggaaga acaagggcag gttcattttg
    31261 tgggcgatag cctgcagcgg attatgggta acaacgtgca attcaatatt gtatatatca
    31321 aaaaatcggg agatacgcta tatctcgaca ctgagattat tcaaaacgac agcttacttt
    31381 ttcacggtgg tattatcgat ttgaatggca acgatttgtt tttgagcgac tacgacttac
    31441 atcataaagg ccgtttgggg caagaatccg actcgtcaca cataatggga ctaacgggat
    31501 atgtttacac acaagccaac atgagcagtt tcgatggata cggtatggga ataaaatttc
    31561 aaaacggctc taccgcaggc aatataacca ttcaacgagg gcacacttca gaaactacag
    31621 taaccgacgg tagtgtaaaa aagtacttta atctgatgcc cggtcaagcc ggaaatcacg
    31681 atgttattgt tcactatctc gacaccaccg atttgtggga tgtgaactgc aacgaaagtg
    31741 actttaaact ctggtcatcg tacaccaatg ggtattacta cgaaaaaaaa tatggtgggg
    31801 tcgatactac cggaaacgtt gccgaaacaa acggagaagt gatttacata caaaatccaa
    31861 cccgcattac cgtagccgac catatttgtg atgttattcc tgtggttaac ataggcgaca
    31921 cactacatat ttgtaacgga agtaccggaa gcattaatgc atacaacaac ggttcttttt
    31981 ttgtatggag tactggcgaa acctctcaaa ttatcagtgt aaacactgct ggaatgtact
    32041 atgttaccgt taccgaccca agaggctgtt tcaatatcga ctcggtattg gtggttctcg
    32101 actcggttcc tcacacaaac ttctcgttta actcgccggt gggtccttgc ccgggaactc
    32161 ttataagttt caacaacacc actacagtgg ctcctgaagc agagccatta agttatctgt
    32221 ggcaatttgg cgatgggaat actgctaccg aagaatttcc gcaacacgca tatgtagccg
    32281 gcggcaccta taccattact cttacggcca ttgcagccaa ttcgtgtgcc atcagccata
    32341 cagccacagt gcacgttttc gatttacccg aaatgagttt tacgtttaat aatgcttgca
    32401 agaacaagcc ggtaattctg accaatacaa ccccaaatgc attccccggt ctaacacatt
    32461 ggaatttggg caatggagac cagttttttg cagaagatac tgcctatttc tataccaatc
    32521 acgggactta tgcagtgcaa ttaatagcaa ccgataatca cggttgcacc gacacaatca
    32581 cacaaaatat agaagtttat ccattaccca tagccggttt cacaaccgac gacgccgatg
    32641 tttgtttgca aaactcgtcg gtctttcaca actcctcaag cattccatac ggtaatttaa
    32701 gttatcaatg gcaatttgga aacggatttg caaataccac tccaaatcca ataattgtat
    32761 acgacacaca tggtaactat accgttcagc ttattgcaac cagctcttat ggctgtagcg
    32821 acacggctta caataatgta attattcgcc ccattcccaa tgcggaattt agttttagca
    32881 atgtgtgcga gggcgacacc gtgtttttta ccaacgaaag cagcatcgac ccaagcgaaa
    32941 tacttaccta tcactggaca tttggagacg gtagcagttc aaacattgca aaccctaaaa
    33001 aattatacag caaccccggt tcctacactg tgaacctaga agtaacctcg gagcagggct
    33061 gcagcgagag tattcaaaaa accgttcagg tttatgccaa cccgaatgcc aactttttgt
    33121 gccaaccggt gtgttttggc aataccagta tgtttcaaaa tatgtcgctc cccaacaaca
    33181 gcagtcttac attctattgg gaatttggcg atggctcgca atcggatgcc tcaaacccgc
    33241 aacattatta taatgccgaa ggagaatatt tggtgagttt aattgctgaa tcgggagtag
    33301 gttgctccga caccattgat aaaatagtgc gtgtttatcc cactcctgtt gtcgatttgg
    33361 gcgataatat atttcattgt ttcaactctt atgttctcga tgcccagaac agtggggcaa
    33421 actatatttg gtcgaacaat gctgcaagtc agacgattac tgtaaatcaa aatggagatt
    33481 attcggtaac cgtaaccaat aactatggct gctcggccag cgaccaagtc cacgtaacat
    33541 taaacgctcc aatagttgta agtttcgacg gacagtattg ggaggtatgt gactcaacga
    33601 ctttgaatgc aggttacccg ggagctgatt ttatttggtc aaccggtgct agcacgcaaa
    33661 gcataacagt attagaatcg ggaatatatg ctgtaactat taccaatcag ggatgtaccg
    33721 gtgttggaag tgtagaaata gtggtacacc aatcgccaca actaaacctg ggcgacgttg
    33781 ttaccgaatg tgaaggtttg gcagtagtgc ttgatgccga aaatgaggga tcaaattatc
    33841 tgtggtcaac caaccaaacg ctgcagcaaa ttgaagtgaa tcaggctggc acatattctg
    33901 ttacggttac cgacattaat tcgtgtacgg ttagcgacag cgttcaggta gtgtttaata
    33961 gtcttcctat tgaccctttc cccgccgaca ccattatatg cggaacaggt tttttaaacg
    34021 cacacaatca gggtgcagca tttgtatgga ataccggtag cactgcccaa gctatcgaaa
    34081 taagccaatc gggcaattac tgggtaacaa ttaccggtgg caatagttgc agcttaatcg
    34141 atacggtaag tgtaactgca tatccccgac ctttggttga tattggcaac gataccgctc
    34201 tatgtaacga agaaattctt gttttgagtg caggaaa
//
LOCUS       DAOOQP010000017        34036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1484_length_34036_cov_6.000971, whole genome shotgun sequence.
ACCESSION   DAOOQP010000017 DAOOQP010000000
VERSION     DAOOQP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1484_length_34036_cov_6.000971"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLK75_03175"
     CDS             complement(<1..1003)
                     /locus_tag="PLK75_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOY38278.1"
                     /translation="MAKDLKFNLESRDLLKKGVNTLADAVKVTLGPKGRNVVIEKKFG
                     APQVTKDGVTVAKEIDLKDPFENMGAQMVKEVASKTNDDAGDGTTTATLLAQAIINTG
                     MKNVAAGANPMDLKRGIDKAVQVTIENLQSQTRSIKKNDIINKAEVIFKESKEKKHEI
                     SHAEKAKIINEIVDVDEISQVGSISANNEKEIGVTISYAMWKAGHEGVITIEEAKGTE
                     TVVKVVEGMQFDRGYISPYFINDSEKMEAVLENPYILITDKKISSMKDLMPVLEPVAQ
                     EGSSLLIVAEDVEGEALATLVVNRLRGTIKIAAVKAPGFGDRRKEMLQDIAVITNGEV
                     "
     gene            complement(1022..1300)
                     /locus_tag="PLK75_03180"
     CDS             complement(1022..1300)
                     /locus_tag="PLK75_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOY38279.1"
                     /translation="MTKIGRPLSGKVLVKPEPAEQKTASGIIIPDSAKEKPQRGRVVA
                     VGKPKKDEENEVSVDDIVLYGKYSGTEIKIDNDDYLILNQNDILLIIQ"
     gene            complement(1488..2777)
                     /gene="eno"
                     /locus_tag="PLK75_03185"
     CDS             complement(1488..2777)
                     /gene="eno"
                     /locus_tag="PLK75_03185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOY38280.1"
                     /translation="MGQIANVHARQILDSRGNPTIEVEVYTTTGVIGRAAIPSGASTG
                     VHEAVELRDNDPSMYGGKGVLQAVHNVTETINELLNGIDPSDQRLIDMMMIDADGTEN
                     KSSLGANAMLGVSLACAHAAAQEHGLPLYNYLGGVNAHVLPIPMMNILNGGKHADNLI
                     DIQEFMIMPVGADTFADALRMGTEVFHQLKSVLKKNKFVTNVGDEGGFAPNLKSNEEA
                     IEYVVQAIDKAGYKPGEDIYIALDPAASEFFDSKKNLYIFESNGSKMTPAEMVSYWEN
                     WVSKYPIISIEDGLAEDDWEGWKLITDKLGYKIQLVGDDLFVTNVNRLKKGIDEKTAN
                     SILIKVNQIGTLTETINCIQMAQGVGYTTVISHRSGETEDTFIADLAVAMGTGLIKTG
                     SASRSDRIAKYNQLLRIEEHLGDAASYLGRDFRFLKK"
     gene            complement(2857..3420)
                     /gene="rplQ"
                     /locus_tag="PLK75_03190"
     CDS             complement(2857..3420)
                     /gene="rplQ"
                     /locus_tag="PLK75_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOY38281.1"
                     /translation="MRHGKNFNHLGRQSAHRKAMLSNMAVSLLLHKRINTTVAKAKAL
                     RMYIEPIITQAKEDTTHNRRVVFSELQDKKAVSELFREVIVKIGERPGGYTRILRTTS
                     RLGDGAEMCMMELVDYNESLLGTKSAQKKSRRSRKKATPAGKAAEPKKKADSSEKETT
                     KKPEAKAKKAPAKVSKPKAEKPDKTAE"
     gene            complement(3432..4424)
                     /locus_tag="PLK75_03195"
     CDS             complement(3432..4424)
                     /locus_tag="PLK75_03195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOY38282.1"
                     /translation="MAILAFQKPDKVIMLEADEFKGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAITTVKIDGVDHEFSSIKGVMEDVTEIILQLKQIRFKRQIEDFSHEKVTV
                     VISGQDEFKAGDIGKFLTGFKVLNPGLLICRMEPSTTLRMEFVISKGRGYIAPDENKP
                     IEDEIGVIPIGSIHTPVVNVKYQIENMRVEQKTDYEKLVLDIETDGSIHPKDALKEAA
                     TILIYHFMLFSDEKITIDADEKMNSEEFDEEVLHMRQLLKTKLVDLDLSVRVLNCLKA
                     AEVETLGDLVQFNKNDLLKFRNFGKKSLTELENLLDNLSLNFGMEISKYKLDKD"
     gene            complement(4433..5041)
                     /gene="rpsD"
                     /locus_tag="PLK75_03200"
     CDS             complement(4433..5041)
                     /gene="rpsD"
                     /locus_tag="PLK75_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOY38283.1"
                     /translation="MARYRGPQSKIARKFGEPIFGPDKYLDKKNYPPGFHGLNKRRKK
                     TSEYGVQLMEKQKVKYTYGILERQFRKIFHSAQSSKGVTGVVLLQLLESRLDNLVYRM
                     GMAPTRAAARQLVSHRHVVVNNEICNVPSRIIVPGEIIGVREKSKSLEAIANALSGYN
                     HNKYSWIEWDSEKMMGKLISRPEREQIPENIKEQLIVELYSK"
     gene            complement(5073..5462)
                     /gene="rpsK"
                     /locus_tag="PLK75_03205"
     CDS             complement(5073..5462)
                     /gene="rpsK"
                     /locus_tag="PLK75_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOY38284.1"
                     /translation="MAKKIKTTKKRVVKVEPTGQAHVYSSFNNIIVTLTNNDGQVISW
                     SSSGKKGFKGSKKNTPYAAQTAAEDCAKFAYDLGLRKVKVFVKGPGAGRESAIRSIHN
                     MGIEVMEIVDITPLPHNGCRPPKRRRV"
     gene            complement(5474..5848)
                     /gene="rpsM"
                     /locus_tag="PLK75_03210"
     CDS             complement(5474..5848)
                     /gene="rpsM"
                     /locus_tag="PLK75_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOY38285.1"
                     /translation="MARIAGIDIPSKKRGEVALTYIYGIGRSRAREILTRAGIDLDIK
                     ADDWNDAQIAEIRRIAGEFRLEGELRSEVQLNIKRLQDIGCYRGIRHRIGLPLRGQKT
                     KTNARTRRGKKKTIANKKKATK"
     gene            complement(5872..5988)
                     /gene="rpmJ"
                     /locus_tag="PLK75_03215"
     CDS             complement(5872..5988)
                     /gene="rpmJ"
                     /locus_tag="PLK75_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOY38286.1"
                     /translation="MKIRTSVKKRSADCKIVRRKGRIYVINKKNPKYKQRQG"
     gene            complement(6003..6221)
                     /gene="infA"
                     /locus_tag="PLK75_03220"
     CDS             complement(6003..6221)
                     /gene="infA"
                     /locus_tag="PLK75_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOY38287.1"
                     /translation="MAKQASIEQDGTITEALSNAMFRVELQNGHVITAHISGKMRMHY
                     IRILPGDKVRVEMSPYDLTKGRITFRYK"
     gene            complement(6227..7009)
                     /gene="map"
                     /locus_tag="PLK75_03225"
     CDS             complement(6227..7009)
                     /gene="map"
                     /locus_tag="PLK75_03225"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOY38288.1"
                     /translation="MIFIKTAEEIVLMRESNLLVSKTHALLAREIKEGVTSNYLDKIA
                     NEFIRDNGAVPGFLNYNGYPKSLCISVNDVVVHGIPNDVPLKYGDIVSVDCGVYKNGF
                     HGDSAYTFTVGKVSKEIDKLLEVTKKALYLGIAQAVAGKRVGDIGHAIQTYTESFGFS
                     VVRELVGHGIGRKLHEKPEVPNYGKSGQGIKLVENMAIAIEPMINMGKKEVKQMHDGW
                     TIKTADGKPSAHFEHTVIVKKDKAEILSNFEIIEKELNRESK"
     gene            complement(7028..8362)
                     /gene="secY"
                     /locus_tag="PLK75_03230"
     CDS             complement(7028..8362)
                     /gene="secY"
                     /locus_tag="PLK75_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOY38289.1"
                     /translation="MKRFIETLKNIWKIEDLRGRILLTLGYIAIYRLGSYIVLPGIDP
                     SQLTNLKAQTADGVLSLLNMFSGGAFSNASVFGLGIMPYISASIVVQLLGIAIPYFQK
                     LQREGESGRKKMNQITRYLTVAILLLQAPTYIANLNSQLPATAFLYTGFMFKFSSVVI
                     LTAGTMFVMWLGEKITDRGIGNGISLIIMIGIISRLPFSFAAEFVSRLEEQTGGLVMF
                     LVEMVALFVVFMLSIALVQAVRRIPVQYAKRVVGNKQYGGVRQYIPLKINAAGVMPII
                     FAQALMFVPLMFAGFSSTTSGFVAAFSDYTGFWYNFVFAVLIIVFTYFYTAVTINPQQ
                     MAEDMKKNGGFIPGIKPGKKTVDFLDNIMSRITLPGSIFLAIIAILPSIAMLAGINNQ
                     FAGFYGGTSLLILVGVVLDTLQQIESHLLMRHYDGLTKSGRIKGRGSYTSAM"
     gene            complement(8366..8812)
                     /gene="rplO"
                     /locus_tag="PLK75_03235"
     CDS             complement(8366..8812)
                     /gene="rplO"
                     /locus_tag="PLK75_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOY38290.1"
                     /translation="MDLSSLKPAKGSVKNKKRIGRGQGSGKGGTSTRGHKGAQSRSGY
                     KKTRGFEGGQMPLQRTSPKFGFKNMNRVEYKAINLSTLQYLAEKCKIDSIDYGLLLEN
                     GLVAKKDKVKILGNGNLTIKVSVKAHAFSKAAVQKIEALGGIAEKI"
     gene            complement(8833..9012)
                     /gene="rpmD"
                     /locus_tag="PLK75_03240"
     CDS             complement(8833..9012)
                     /gene="rpmD"
                     /locus_tag="PLK75_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOY38291.1"
                     /translation="MAKIKITQVRSKIGCPPNQKNTLIALGIKKMHQTVEHEATPQIL
                     GMVKAVHHLVKVENI"
     gene            complement(9023..9538)
                     /gene="rpsE"
                     /locus_tag="PLK75_03245"
     CDS             complement(9023..9538)
                     /gene="rpsE"
                     /locus_tag="PLK75_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOY38292.1"
                     /translation="MSSSIRKVKTSDLELKDRLVGIQRVTKVTKGGRTFSFSAIVVVG
                     NENGIVGYGLGKASEVTAAIAKGVEDAKKNLIKVPVVKGTVPHEQVAKFGGAKVWLKP
                     ASSGTGVKAGGAMRAVLESVGVTDVLAKSKGSSNPHNLVKATIAALIEMRDANTVAQH
                     RQINIEKVFNG"
     gene            complement(9548..9904)
                     /gene="rplR"
                     /locus_tag="PLK75_03250"
     CDS             complement(9548..9904)
                     /gene="rplR"
                     /locus_tag="PLK75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOY38293.1"
                     /translation="MAFSKQNRRIKIKNRIRKHVNGTPIRPRMTVYRSNKQISVQLID
                     DINGVTLAASTSMIKNIAEQDANKTIKAKMVGKAIAQKAIEAGITEVVFDRNGYLYHG
                     RVKSLAEGAREGGLKF"
     gene            complement(9923..10474)
                     /gene="rplF"
                     /locus_tag="PLK75_03255"
     CDS             complement(9923..10474)
                     /gene="rplF"
                     /locus_tag="PLK75_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOY38294.1"
                     /translation="MSRIGKLPIQLPANVTITTSDNIVTVKGPLGELSQKIHNSTEVE
                     VQGNEVIVKRKSEERNDRASHGLYRALIANMIEGVSQGFTIQQELVGVGYKADSKEQI
                     LELSLGYSHHIQFMMPKEVQVECKTEKRNNPIITLKSHDKQLLGQVAAKIRSFRKPEP
                     YKGKGIKFVNEELRRKSGKSASV"
     gene            complement(10485..10880)
                     /gene="rpsH"
                     /locus_tag="PLK75_03260"
     CDS             complement(10485..10880)
                     /gene="rpsH"
                     /locus_tag="PLK75_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOY38295.1"
                     /translation="MTDPIADFLTRIRNAVMAKHRVVEIPASNLKKEMTKVLFEKGYI
                     LNYKFEEDGPQGSIKIALKYHPVSKIPAIKNLTRISKPGLRKYVPSNELPRVLNGLGI
                     AILSTNKGIMTDKEARQLNVGGEVLCYIY"
     gene            complement(10899..11168)
                     /gene="rpsN"
                     /locus_tag="PLK75_03265"
     CDS             complement(10899..11168)
                     /gene="rpsN"
                     /locus_tag="PLK75_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOY38296.1"
                     /translation="MAKESMKAREVKRQKLVMKYAAKRARLKKEGEYQALQDIPKNAS
                     PVRMHNRCSQSGRPKGFMRQFGVSRIVFREMASKGLIPGVKKASW"
     gene            complement(11170..11727)
                     /gene="rplE"
                     /locus_tag="PLK75_03270"
     CDS             complement(11170..11727)
                     /gene="rplE"
                     /locus_tag="PLK75_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOY38297.1"
                     /translation="MDYKPNLMKKYQEEVIPELKKEFGYKSVMQIPKLEKIVINQGLG
                     QAIADKKILEIAIEELTMITGQKPVTTLSKKDVSNFKLRKKMPIGLTVTLRREKMYEF
                     LERLIAIALPRIRDFKGVNYKFDGRGNYTLGISEQIIFPEIDIDKIYKIMGMDITFVT
                     STKNDEEAYALLKYFGFPFKNLKKN"
     gene            complement(11727..12050)
                     /gene="rplX"
                     /locus_tag="PLK75_03275"
     CDS             complement(11727..12050)
                     /gene="rplX"
                     /locus_tag="PLK75_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOY38298.1"
                     /translation="MSRKFHVKKGDTVVVIAGESKGQQGRVLSVNREDERAIVEGVNL
                     ISKHTKPNAKNTQGGIVKKEAAVHISNLMIADPKTGKPTRTGRKIGKNQKSVRYSKKS
                     GEEIK"
     gene            complement(12069..12437)
                     /gene="rplN"
                     /locus_tag="PLK75_03280"
     CDS             complement(12069..12437)
                     /gene="rplN"
                     /locus_tag="PLK75_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOY38299.1"
                     /translation="MIQQESRLIVADNSGAKEVLCIRVLGGTRHKYASIGDTIVVAVK
                     TALPGGDVKKGTVSKAVIVRTKKEVRRSDGSYIRFDDNACVLLNNAGEIRGTRIFGPV
                     ARELREKQYMKIVSLAPEVL"
     gene            complement(12440..12697)
                     /gene="rpsQ"
                     /locus_tag="PLK75_03285"
     CDS             complement(12440..12697)
                     /gene="rpsQ"
                     /locus_tag="PLK75_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOY38300.1"
                     /translation="MENRNLRKERIGVVVSNKMEKSIVVAVKRKEKHPKYGKFVNKTK
                     KFIAHDEQNTCDIGDVVRIMETRPLSKNKNWRLVEIVEKVK"
     gene            complement(12710..12910)
                     /gene="rpmC"
                     /locus_tag="PLK75_03290"
     CDS             complement(12710..12910)
                     /gene="rpmC"
                     /locus_tag="PLK75_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOY38301.1"
                     /translation="MKNTEIRELSEKEIIERIEETKASLIRLKMNHVVSPLDNPQKLV
                     QVKRTIARLNTELRKRQLQNKK"
     gene            complement(12921..13346)
                     /gene="rplP"
                     /locus_tag="PLK75_03295"
     CDS             complement(12921..13346)
                     /gene="rplP"
                     /locus_tag="PLK75_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOY38302.1"
                     /translation="MLQPKKTQYRRQQKGKMKGNAQRGHEIAFGSFAIKSLESAWITG
                     RQLEAARVAVTRKMQREGQIWIRVFPDKPITKKPAEVRMGKGKGAPEGFVAPVTPGRI
                     LFEADGVSIELAKEALRLAAQKLPVKTSFVVRRDYNFNE"
     gene            complement(13371..14075)
                     /gene="rpsC"
                     /locus_tag="PLK75_03300"
     CDS             complement(13371..14075)
                     /gene="rpsC"
                     /locus_tag="PLK75_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOY38303.1"
                     /translation="MGQKVNPIANRLGIIKGWDSNWYGGRKYADKLAEDASIRRYLNA
                     RLAKASVSSIVIERSLKMITITIKTARPGIIIGRGGQEVDTLKEELKKLTNKEVQINI
                     FEIKRPELDANLVANNIARQIEGKVAYRRAVKMAIASTIRMGAEGIKVQVSGRLNGAE
                     MARSEMYKEGRTPLHTLRADIDFSLGEALTKTGLIGVKVWICRGEVYGKRDLSPSIEV
                     KGAGKKVIKKQRNTKR"
     gene            complement(14086..14532)
                     /gene="rplV"
                     /locus_tag="PLK75_03305"
     CDS             complement(14086..14532)
                     /gene="rplV"
                     /locus_tag="PLK75_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOY38304.1"
                     /translation="MGARKRNRAEKMKEERRNISFAELNNCPTSPRKMRLVADLVRGE
                     DVNKALDILKYSNKYASGKLEKLVMSAVANWQAKNEGKRMEEAGLYIKEISVDEGRSL
                     KRMQPAPQGRAYRIRKRSNHVTVVIGSRNLVEQEHEEQETIVENEN"
     gene            complement(14553..14819)
                     /gene="rpsS"
                     /locus_tag="PLK75_03310"
     CDS             complement(14553..14819)
                     /gene="rpsS"
                     /locus_tag="PLK75_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOY38305.1"
                     /translation="MSRSLKKGPFIDVKLEKKVLTMNSGSKKSVIKTWSRASMISPDF
                     VGHTFAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHGGKKK"
     gene            complement(14831..15655)
                     /gene="rplB"
                     /locus_tag="PLK75_03315"
     CDS             complement(14831..15655)
                     /gene="rplB"
                     /locus_tag="PLK75_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOY38306.1"
                     /translation="MAVRKLKPTTPGQRFKVISTFDEITHIVPEKSLLEPIKKTGGRN
                     NQGRMTMRYIGGGHKRRYRIIDYKRDRDGVKAIVKTIEYDPNRTARIALVCYEDGEKR
                     YIIAPNGINVGQVLETGKNVAPEIGNTLFLSDIPLGTIVHNIEMRPGQGGVLARSAGT
                     YGQLTSREGKYAILRMPSGETRKVLTTCKATIGSVSNSDHGLEKLGKAGRTRWFGRRP
                     RVRGVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRDRKKPSNKFIVERRKK"
     gene            complement(15661..15951)
                     /gene="rplW"
                     /locus_tag="PLK75_03320"
     CDS             complement(15661..15951)
                     /gene="rplW"
                     /locus_tag="PLK75_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOY38307.1"
                     /translation="MEIILRPIVTEKMMAQSDKLHRYGFIVDKTANKIQIKAAVETLY
                     GVSVMEVNTMRYGGKAKSRFTKTGLVKGKTNSFKKAVVTLKEGEVIDFYSNI"
     gene            complement(15964..16590)
                     /gene="rplD"
                     /locus_tag="PLK75_03325"
     CDS             complement(15964..16590)
                     /gene="rplD"
                     /locus_tag="PLK75_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOY38308.1"
                     /translation="MKLPVYNKSGKETGNEIDLNDSIFAIEPNDHAIYLACKQYMANN
                     RQGTHKSKERSEIAASTRKLKRQKGTGGARAGGMNNPLFRGGGRVFGPQPRDYSFKLN
                     KKVKKLARKSALSYRINSNGIVIVDNLLFEDVKTRNIIDLVNNFNLSNKKMILVVKEP
                     NNNIYLSSRNLKNVKVITASDLSTYDIMWASTLMFEQESIDVLEKIIA"
     gene            complement(16590..17231)
                     /gene="rplC"
                     /locus_tag="PLK75_03330"
     CDS             complement(16590..17231)
                     /gene="rplC"
                     /locus_tag="PLK75_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOY38309.1"
                     /translation="MQGIIGKKIGMTSIYSAEGKNIPCTVVEAGPCVVTQIRSIEKDG
                     YIAVQLAFDDKKDKHTTKAMQGHFAKAGTTPKKKVVEFKKFGKIRVNDELRDPVVGDI
                     IDVGIFIEGEFCDVSGITKGKGFQGVVRRHGFSGVNDATHGQHNRGRAPGSLGASSWP
                     SRVFKGMRMAGRMGGKKVKMISLRVLKVIPQSNLVLIKGSVPGAIGSYLIIER"
     gene            complement(17255..17560)
                     /gene="rpsJ"
                     /locus_tag="PLK75_03335"
     CDS             complement(17255..17560)
                     /gene="rpsJ"
                     /locus_tag="PLK75_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOY38310.1"
                     /translation="MGQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAIVCGPIPLPTH
                     KRIFTVNRSTFVNKKAREQFELSSYKRLLDIYSTTAKTIDALMKLELPSGVEVEIKV"
     gene            complement(17584..19692)
                     /gene="fusA"
                     /locus_tag="PLK75_03340"
     CDS             complement(17584..19692)
                     /gene="fusA"
                     /locus_tag="PLK75_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOY38311.1"
                     /translation="MPEVLSHIRNIGIMAHIDAGKTTTTERILYYTGVNYKIGEVHDG
                     AATMDWMQQEQERGITITSAATTAFWKYDENKYTINIIDTPGHVDFTVEVERSLRVLD
                     GAVAVFCGVGGVEPQSETVWHQADRYHVPRIAFVNKMDRVGSDFQSVVAQINEKLGAN
                     AVAVQIPIGFEDGFCGVVDLITNKAYVWDDTNGSLGVNFDIQDVPENLKEHAEEMREA
                     MIDKVMAISDDQLLEKFLDDRKQVSVDEFKLAMRKAVISGQLVPVLCGSSFKNKGVQL
                     LLDAICAYLPSPSDISAVAGINPRTEQEAVRHPSEDEPFAGLCFKIASDPFFAKLTYV
                     RIYSGKVSSGQMIYNSRTGKKDRASRLFQMHANKQLPINEAKAGDIIAVVGLKDVKTG
                     DTICDENKPISLEAMNFPEPVILMAVEPEMQADIEKLSSALNRLQEEDPTFNVKYDEQ
                     SGQTLISGMGELHLDIITDRLKREFGLKINKGAPRVNYKEELTESIEYHMIFSKQTGG
                     KGKFAELTLKFEPIQQNGFTGLQFENCIKNGSIPKEFIPAVEKGLKEAMQNGPISGYP
                     LNSLKASLLDGSYHSVDSDALSFEIAARKAFSEASRKVKSVILEPITKTEVVTPEEYV
                     GDVVADFNRRRASVTGLDSRGMVRVIKAEVPLAEQFGYVTALRTITSGRASFSMEFSH
                     YAVVPALIQEEIINANKYTF"
     gene            complement(19701..20177)
                     /gene="rpsG"
                     /locus_tag="PLK75_03345"
     CDS             complement(19701..20177)
                     /gene="rpsG"
                     /locus_tag="PLK75_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOY38312.1"
                     /translation="MRKTRPKKRLILPDPKYNDVRVTRFVNDMMWSGKKSTSFEIFYE
                     SLDIVEKKMKDSEDAPLDIWKKALDNITPQVEVKSRRIGGATFQVPTEIREDRKISIS
                     HKWMIGFARKRSGRGMAEKLAAEIIAAYNNEGAAFKKKEDTHRMADANKAFSHFRF"
     gene            complement(20202..20594)
                     /gene="rpsL"
                     /locus_tag="PLK75_03350"
     CDS             complement(20202..20594)
                     /gene="rpsL"
                     /locus_tag="PLK75_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOY38313.1"
                     /translation="MPTIQQLVRKGRLALIDKSKAPALDSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHVVRGAL
                     DTAGVDGRKQRRSKYGAKKKKAAAAKKK"
     gene            20870..21991
                     /locus_tag="PLK75_03355"
     CDS             20870..21991
                     /locus_tag="PLK75_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38314.1"
                     /translation="MKFLTVPIVSALFCCLLLINSCIEPVPPSDKLEVVLVSSNITTP
                     TTWAGDKAYVIQSSDFYVEAALTIEPGAVVKFSGQNAYLTLGAEGAINAVGTSEKPII
                     FTSLYDDEHGGDSNDDSDATSPQAKDWGTIDLNGTQGSVFNYCEFRYGGRGATPEPTL
                     NLSADASASVTNCMFIDNGGGIRTSGNLTSYIGVVHAESANNQTIIRNNLFYRNVVPL
                     VIFAEISIDNSNSFSYYANSNTMNGIFVSGVTMMKTTSWLETEVAYVITSGDLSINEP
                     YKLVLGNDVVLKFVNDSRLNCGGGEIALENHSGDGVFFTSYKDDNYKGDSNGDGANSS
                     PAEADWIGIFLDDYKGGFADWPNILYNDPDALVKSTGVN"
     gene            complement(21988..22929)
                     /locus_tag="PLK75_03360"
     CDS             complement(21988..22929)
                     /locus_tag="PLK75_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOY38315.1"
                     /translation="MIDLNTIAEASRRIAPYIHVTPILSNKTINSMVGGTILFKCENF
                     QKAGSFKIRGACNAVFSLSELQAKNGVCTHSSGNFGQALALAASYRGIQAHIVMPKTS
                     SCVKVEAVKAYSGNVRFCEPNLMSRESTAREVIDATGAAFIHPYDDYIIMSGQGTAIA
                     EVMRSEQNIDIVMTPVGGGGLISGTAVAAKSLNRKIWVIAAEPIQANDAFRSFYAKKW
                     FPSENPDTIADGLLTSLGERNFALILNFVDDILTCREKSIYEAMRLIWERMKIIIEPS
                     SAVPLACVLENKGIFENKTTCIVLSGGNIDLNRNWFS"
     gene            complement(22926..23294)
                     /locus_tag="PLK75_03365"
     CDS             complement(22926..23294)
                     /locus_tag="PLK75_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOY38316.1"
                     /translation="MANHNDTGNKGEMLAAQYLISHGFEILSTNWHSQHREVDIIARK
                     NGLIVFVEVKTRSSDQWQRPEESVDARKQKLLIEAAGDYMANYPDDAEARFDVVSVVL
                     NANGDVKITHFPEAFIPELS"
     gene            complement(23337..24062)
                     /locus_tag="PLK75_03370"
     CDS             complement(23337..24062)
                     /locus_tag="PLK75_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38317.1"
                     /translation="MKIAFTSLLIIFANVILLAQSEILIKDHDGNIVNGQTITVQGNI
                     ALNLNSAIIYFYIQNNTSASLDVRCRKEEISVIPNTMSDICWNTTCYPSNTFSAPPYT
                     LAAGATTDEAHKFTAHYHCQGNSGTTSMRYYFFVSGGGPESYVNVDFVVNSSSVNYNT
                     LSATSVYPNPADGFFYIDAADVEGAKLQVEVYNMIGRKILDKKFQNNNNLVVDCSTWE
                     KGVYFVRLYSDGILMKTSKLTKK"
     gene            24239..25942
                     /locus_tag="PLK75_03375"
     CDS             24239..25942
                     /locus_tag="PLK75_03375"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOY38318.1"
                     /translation="MKHIFLFTILLGLFSVAFAQHTIEIELKTDNYGSETTWEVVNTL
                     NNDVLAEGGPYTNVSAVQTIAPIDVDGTGCYAFYIYDEYGDGICCNYGSGYYKVYYDD
                     VMIGQGGEFSSEASVMGMGSGCPQNEIELIANNTLPWTARQGDYAVTGQNTNIKGRII
                     NNGVVTLTSFQVTYNIDGGENLAPQTITCNATMGQTVNFTYNLNPAVFSTDGPYVVNI
                     IVSNPNGVEDDDSDNTSSVNIIANSNSVSRNVLLEHFTTAECPNCPAAITNLTNWTNT
                     RPNIIWLSHHAGYYTDDLTIPENTQLLLFYNAGGSTYAPGIMLDRKFLSPDGDPGPVF
                     FPASSYTTGLMDQMRNEPAYVSLEMEGNYNTETGLLNLTVSGEFLGTVAGTPRLSVYI
                     QEDGLVGEQAGASNNYTHNHSMRDAITANYGDATAIPNGNTGTTFTKTYTYTVDSEWV
                     LGNLTLIAFVNDWNNSNVNDREIYNSIKMPLSDFVSIADEYSENIMIFPNPASDMITI
                     TGVENASVNIFSVTGQLVFSDNCNSNEITVTTANLSEGTYIVKVVKDSSTIVKKVIVT
                     K"
     gene            26031..26534
                     /locus_tag="PLK75_03380"
     CDS             26031..26534
                     /locus_tag="PLK75_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY38319.1"
                     /translation="MKKLFFVFVLVVMAGYIFGQEVPQFQIKDINNKTVNTKDIIKNN
                     GKPVVISFWATWCKPCIKELNAYNENYADWQKETGVKIIAISIDDSRSMSKVAPFVNG
                     RMWEFNVYCDPNSDFKRGMNVNEVPHTFVLDKNGKVVWQHKAYVEGDENTLHEVIQKV
                     AAGKPLK"
     gene            26581..28212
                     /locus_tag="PLK75_03385"
     CDS             26581..28212
                     /locus_tag="PLK75_03385"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOY38320.1"
                     /translation="MAIALLPFSFVWAQEGTWGQITGNFQADGQYYIADSIIGASSPA
                     EKTMLNSFANFTYNLGNFSAGFRYEGYLNTLLGYPNQGGINDGIGIPYRWANFVNDKF
                     EITVGNFYEQFGNGLVLRTYEEKQLGIDNALDGIRVKISPYKGIYLKGLVGKQRLYWE
                     KGEGIVRGFDGDFMVNEIVPEWNEKPWNLGFGGSFVSKYQADEDPVYALPQNVGAGAG
                     RLLFSVAGLSINSEYAYKINDPSSDNGYIFRPGQAFLVNASYSRSGLGILVATKWIDN
                     MSFRSDRNAISTNLNINQLPEITKAHTYSLTAFYPYATQPNGEWGVQADVIYKFKKNT
                     KIGGKYGTSIALNFSRVQNTAQTPVNDSTPIGSHGTYGYNTNFFSIGDSLYFQDINFE
                     LSKKWSSKFKTIFTYQNIIYNYNVLRGTRGHETVFAHCGIADISYNFTDNISLHTELQ
                     ALFTKQDKGNWGMLLLEFTIPHWFFTISDNWNYGNSDASHRTHYLLAGFGYNNGGNRI
                     QLSYGKQREGITCVGGVCRAVPASNGIIISISSTF"
     gene            28223..29080
                     /locus_tag="PLK75_03390"
     CDS             28223..29080
                     /locus_tag="PLK75_03390"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOY38321.1"
                     /translation="MKKLFIITFCLLASAFFTTCDIIEAPYLDTNEPVWNGRKSLILD
                     FTGHMCGNCPYAHRTIVSMQNNFGEAVVPIAVHCGYFSMLASNDPLQPYHYDFTTDVG
                     LELGGNGFTSYGYFGVQSQPIGVVNHLIPESLKAHDGWGSEIAKYYSSFPELMIHVDK
                     TFNETDSTLIAVVHVESQIESTRNLSLAVYITESHIIQWQTDYSQTDSNVEDYEHNHV
                     LRGSFNGAWGDAINTNNNPITRLQQFEKEYSLKTGLDWVPANLSIIAFVYDTDSKEVL
                     QCEEIHVGE"
     gene            29080..29727
                     /locus_tag="PLK75_03395"
     CDS             29080..29727
                     /locus_tag="PLK75_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY38322.1"
                     /translation="MRSFFLILFFTLISLTQAYAQQFYAGAIGGLVLSQIDGDSYGGY
                     HKFAGSGGLYVRNNFGEKWGYRMELKYIQKGSKAARLDMGYHTIPFYKASLNYIEIPF
                     LFSYRMQKFKIPPTIDWQLDGKLHLNIGMNLSYLFSATEDDGTGPNEAGFAFRRIELG
                     AQTGVTYYFSPHWAFDFRFCYTIIPIRDRPNASDLDYWVTYEFNRVLLFSIMYEI"
     gene            29724..30308
                     /locus_tag="PLK75_03400"
     CDS             29724..30308
                     /locus_tag="PLK75_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOY38323.1"
                     /translation="MKTKKSLVIAITGASGSIYASTLISDLLLLRDYIDPERLSLVVS
                     DNGDQVFEHEMGYRASAISGIKHYSNTDFYAPFASGSGNYDGMVIVPCSMGTLGKIAH
                     GISDNLITRTADVMLKERNKLILVTRETPISLIHIKNMELITLAGGIVCPANPSFYSK
                     PLQVHDLVKTVTNRIISLLGIECNSFQWPGTFTP"
     gene            complement(30298..30993)
                     /locus_tag="PLK75_03405"
     CDS             complement(30298..30993)
                     /locus_tag="PLK75_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOY38324.1"
                     /translation="MKNIEKWACDLVYLLFPEVCEVCMAPLVTGEKTICLKCLADMPY
                     TDYHNKPENPVSQIFWGKVHVEMATALFYFAKGSKYRKLIHKLKYKGKREIGIFLGRE
                     LGACIGKSDYFKGIDCIIPVPLHISRQTKRGYNQSAEIALGISQVTGIPVKLHELIRS
                     QATETQTKKTRDERWKNVSGKFQIASPENLKGKHVLVVDDVITTGSTIEAVVETLLGV
                     EGVTVSIAVVAKA"
     gene            31105..32970
                     /locus_tag="PLK75_03410"
     CDS             31105..32970
                     /locus_tag="PLK75_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOY38325.1"
                     /translation="MKKMILLMVIGAVLTGSIHAATVSGIITDLTTGTTIGNAPVVIA
                     KTNGLSLKMTQSDAFGYFEFKDLSAGDYLIKVNYENYEEYELAFTMAEEENKNLFVYL
                     SVQPSLTDVLFNNKDIEEHKQDCSKTLYSRESSAAPCGGAGYNSFAYDEPAIDWNTES
                     YDYINENKFKTVTNDPLSTFSIDVDRASYSNVRRFITQNQMPYADAVRVEELINYFDY
                     TYKQPTGEHPFSTTLELGTCPWNKDHQLLLIGIQGKKMISEEIPANNLVFLIDVSGSM
                     SDINKLPLLKQSFKILIDQLRPQDRVAIVVYAGAAGQVLASTSGKDKAKISAALDLLE
                     AGGSTAGGAGINLAYKIAKENYIPNGNNRVILATDGDFNVGASSDGEMERLIEQKRND
                     GIFLTILGFGMGNYKDSKMEKLSNKGNGNYAYIDNIMEAKKIFSEELWGTLFTIAKDV
                     KIQIEFNPSKVKAYRLIGYENRLLNKEDFNDDTKDAGEIGSGHSVTALYEIIPGESGE
                     PLASVDPLTYQDVNIRKSNDLMTLKIRYKDPKGTESKLITSKVASTDLGKISDNFKLA
                     ASLAEFGMLLRNSEFKGTATYEDAIRLAKEAMGNDPYGYRAEYVILLEKTKLLSK"
     gene            33079..33149
                     /locus_tag="PLK75_03415"
     tRNA            33079..33149
                     /locus_tag="PLK75_03415"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33111..33113,aa:Gln,seq:ttg)
     gene            33157..33228
                     /locus_tag="PLK75_03420"
     tRNA            33157..33228
                     /locus_tag="PLK75_03420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33190..33192,aa:Gln,seq:ctg)
     gene            complement(33270..33563)
                     /locus_tag="PLK75_03425"
     CDS             complement(33270..33563)
                     /locus_tag="PLK75_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38326.1"
                     /translation="MKAIFIVYNMALTDKVEYMIDVLGIRGYSQWSNMQGRGSETGDP
                     HYGNHTWPEINSAILTIVEDDQVEKILQKVKNLDHINKDVGIRAFVWDITGGY"
     gene            complement(33568..>34036)
                     /locus_tag="PLK75_03430"
     CDS             complement(33568..>34036)
                     /locus_tag="PLK75_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY38327.1"
                     /translation="IPFSFSGVILALYITGTTLSVIAALGAVLLIGIVVKNAIVLVDY
                     INLMRDREYDLYDAIALSGKSRLRPVLMTALTTILGLLPMAMSTGEGSEIWSPMGISV
                     MGGLVFSTIITMIIVPVLYALFTRKGEKLRKRNIHRLKYSFMEDTNDVKEIKE"
BASE COUNT         9408 a         7347 c         6271 g        11010 t
ORIGIN      
        1 taacttcacc attggtaata acggcaatat cctgaagcat ttcttttctt ctgtcgccaa
       61 aacccggagc ctttacagca gctattttaa tggttcccct caagcggtta accaccaggg
      121 tagccaaagc ttcgccttca acatcctcgg caacgataag cagcgatgac ccctcctgtg
      181 caaccggttc caaaacaggc atcaggtctt tcatcgatga aatcttttta tcggtaatca
      241 gaatatacgg attttcgagc acggcttcca ttttttcgct gtcgttaatg aaataaggag
      301 aaatatagcc acggtcgaat tgcatccctt caactacttt aacaactgtt tcggtgcctt
      361 ttgcctcttc gatggtaatt acaccttcgt gaccggcttt ccacatagca tacgaaatgg
      421 ttacaccaat ttctttttcg ttgttggccg atatggaacc aacctgagaa atttcgtcaa
      481 catcaacaat ttcgttaata atttttgctt tttcagcatg actgatctca tgctttttct
      541 ctttgctctc tttgaaaatt acttcagctt tgttgataat atcgttcttt ttaatggaac
      601 gtgtctgcga ttgcaggttt tcgatggtaa cctgaactgc tttatcgata cctcttttaa
      661 ggtccattgg atttgcaccg gcagcaacgt ttttcattcc agtattaata attgcctgag
      721 caagcagcgt tgcggtggtt gtaccatcac cggcatcatc attggttttc gaagcaactt
      781 ctttcaccat ctgagctccc atattttcga aaggatcttt taaatcgatt tcttttgcaa
      841 cagttactcc gtcttttgta acctgaggtg ctccgaattt cttttctatt accacatttc
      901 ttcctttagg gccaagtgtt actttaacag cgtcggccaa tgtgttaacg ccttttttca
      961 ataagtcgcg cgattccagg ttaaacttta aatcttttgc cattgtatat gagtttttaa
     1021 gttattgaat aattaataaa atatcgtttt gattcagaat cagatagtcg tcgttgtcga
     1081 tttttatttc tgtgccactg tatttgccat acagcacaat atcgtcaaca ctgacttcat
     1141 tttcctcatc ttttttaggt ttaccaactg caacaacacg gcccctctgt ggtttttcct
     1201 ttgctgagtc aggaataata attcctgatg cggttttttg ctctgctggt tcaggtttaa
     1261 caagaacttt tcctgaaagt ggtctaccaa tctttgtcat aattctattt tttaaaaggt
     1321 tattatttta ttgatacggc agtttgttat cgaaaaatgt gccatagcca taaatcacca
     1381 aatatctgac aaaattacaa ggtttgtatt gtttgtgttt tttcggcagt gtcaaaatga
     1441 cactacttta accatctatt gcagtagaat tcagtgtata attcaattta cttttttagg
     1501 aatctgaaat ctcttcctaa atatgaagca gcatctccaa gatgttcttc gatgcgaagc
     1561 aattggttat attttgcgat tctgtcggaa cgactggctg acccggtttt aatcaaacct
     1621 gtccccatgg ctaccgcgag gtcggcaata aaagtatctt cggtttcgcc tgacctatgg
     1681 ctgataactg tggtatatcc aacaccctga gccatttgga tgcaattgat tgtttcggta
     1741 agcgttccta tttgattcac tttaattaga atggaatttg cggttttctc atcaattcct
     1801 tttttcaacc ggttcacatt cgtaacgaac aaatcatctc caacgagctg aattttatat
     1861 ccaagcttat ctgtaatcag tttccagcct tcccagtcgt cttcggcaag accatcttca
     1921 atcgaaataa tggggtattt gctaacccag ttttcccaat aggaaaccat ttcagcaggg
     1981 gtcattttac taccattcga ttcaaatatg taaaggtttt ttttgctatc aaaaaattcg
     2041 gaagcagcag ggtcgagtgc aatataaata tcttccccgg gtttgtatcc agccttatca
     2101 atagcttgaa caacatattc gatagcttcc tcgttcgact tcagattggg agcaaagcca
     2161 ccttcatcgc caacattggt aacaaatttg tttttcttta acacagactt tagttggtga
     2221 aacacttctg tgcccattct cagcgcatca gcgaatgtat ctgcaccaac aggcataatc
     2281 ataaattcct gaatatcaat caggttatcg gcatgttttc caccattaag gatattcatc
     2341 attggtatag gcaaaacatg ggcatttact ccgcccaaat agttgtagag aggcagacca
     2401 tgttcctgag cagctgcatg agcgcaggca agcgaaactc caagcattgc atttgcacca
     2461 agagaagatt tattttcagt cccatctgcg tcaatcatca tcatgtcaat gagtcgttgg
     2521 tcgctgggat cgattccatt gagcaattcg ttaatagttt cggttacatt gtgaactgcc
     2581 tgaagaacac ctttccctcc atacatggat ggatcgttat cgcgtaattc aacggcctcg
     2641 tgaacaccgg tcgatgcacc gctaggaata gcagctctgc caattactcc ggttgtggtg
     2701 taaacttcga cctcgatggt aggatttcca cgagagtcta atatttgtcg cgcatgtaca
     2761 tttgcaattt gtcccatttt acttaatttt ttataacatt aaaaataaaa aaaaaagagg
     2821 gagaaaaatc tccctctaat aaaaataatt aaaatattat tcagcggttt tatcaggttt
     2881 ttcggccttt ggtttcgaaa cttttgccgg agcttttttt gcttttgctt ccggtttttt
     2941 tgtggtttct ttttctgatg aatcagcttt cttctttggc tcagcagctt ttccagcagg
     3001 agtagctttt tttctggacc ttcgtgattt tttctgagct gattttgtgc caagcaggct
     3061 ttcgttgtag tcgaccagct ccatcataca catttcggca ccatcgccaa gacgactggt
     3121 ggttctaagt atccttgtat atccacccgg gcgttcgccg attttcacaa ttacttcacg
     3181 gaacagttcc gaaactgcct tcttatcctg taactcactg aatacaaccc ttctgttgtg
     3241 agtagtatct tccttggcct gtgttattat cggctctata tacatacgca aagcttttgc
     3301 ctttgcaacg gtagtattta tgcgtttatg caacaaaaga gatactgcca tattcgacag
     3361 catagcttta cgatgtgcac tctgtcttcc taaatgatta aagttttttc cgtgtctcat
     3421 ctcttttcca attaatcctt atccaatttg tattttgata tttccatccc aaaattcagg
     3481 ctcaaattat cgagcagatt ttccagttcc gtcagcgact ttttgccaaa gtttctgaat
     3541 ttaagcaggt cgtttttatt aaactgcacc aaatctccta aggtttctac ctctgcagct
     3601 tttaaacaat ttaagacgcg caccgacaaa tccagatcca caagtttcgt tttcagaagc
     3661 tgacgcatat ggagtacttc ttcatcgaat tcttcactat tcattttctc atcagcatca
     3721 atggtaatct tttcatcaga aaacaacatg aagtgataaa taagtattgt tgctgcttct
     3781 tttaaagcat ctttgggatg tatagagccg tctgtttcaa tgtcgagaac cagtttttcg
     3841 tagtcggttt tttgctccac cctcatattt tcgatttggt actttacatt cacaacgggt
     3901 gtgtgtatcg acccgattgg aattactcca atttcgtctt cgatgggttt attttcatcg
     3961 ggagcaatat atccgcggcc tttgctaatt acaaactcca tacgtaaggt tgttgaaggt
     4021 tccatcctgc aaattagtaa accggggttc agaactttaa atccggtgag gaattttcca
     4081 atatcgccgg ctttgaattc gtcctgtccg ctaataacaa ccgtaacttt ctcatgtgaa
     4141 aaatcttcta tctgacgttt aaaacgaatt tgcttgagtt gaagtattat ctcggttacg
     4201 tcttccatca cacctttaat gctggaaaac tcatgatcaa caccgtcaat tttaacggta
     4261 gttatagcaa atccttcaag cgaagaaagc aatattcggc gaagggcatt gcctattgta
     4321 ataccgtaac ctggttcaag tgggcgaaat tcaaatttac ctttgaattc gtccgcttcc
     4381 aacatgataa ctttgtctgg tttttgaaat gctaatattg ccatttgctt aattatttag
     4441 aatataactc aacgattagt tgttctttaa tgttctcagg tatttgctct ctctcgggac
     4501 gcgatatcaa tttgcccatc attttttcag aatcccattc aatccatgaa tatttgttat
     4561 gattataccc cgacagtgca tttgcgatag cttccagtga ttttgacttt tcgcgcacac
     4621 caataatttc tccgggaaca attatacgtg aaggcacatt acagatttcg ttgtttacaa
     4681 caacatgtct atggcttaca agttgccttg cagctgccct tgtcggagcc attcccatgc
     4741 gataaaccaa gttatcaaga cgcgattcca acaactgtaa caaaacaaca cctgtaaccc
     4801 ctttgctgct ttgagcgctg tggaatattt tgcggaattg acgttcgaga ataccgtatg
     4861 tgtatttcac tttttgtttt tccattaact gaacaccata ttcagaggtt ttctttctac
     4921 gtttattcaa cccatgaaat ccgggaggat agttcttttt atcgagatat ttatccggac
     4981 cgaagatcgg ttctccaaac ttccttgcaa ttttactctg tggtcctctg taccttgcca
     5041 tactttttta ctgtttaata ttaattttct ggttaaactc ttcttctttt gggaggtctg
     5101 caaccattgt gcggtaatgg tgttatgtca acgatttcca ttacttcaat tcccatatta
     5161 tgaatgcttc taatagcaga ctcacgacct gcgccgggac ctttaacaaa tactttcact
     5221 ttccgaaggc ccagatcata ggcaaacttt gcacaatctt ctgcggctgt ttgagcagca
     5281 tatggtgtgt ttttcttgct tcctttgaat ccctttttac cactcgatga ccatgagatt
     5341 acttgtccgt cattattggt aagtgttaca ataatgttgt tgaaagatga atacacatgt
     5401 gcttgccctg ttggctcaac tttaacgaca cgttttttgg ttgttttaat cttttttgcc
     5461 atgtttcaat caattattta gtggcttttt tcttgttagc aatcgttttc tttttacctc
     5521 ttctggttct tgcgttggtc ttggtttttt gtccacgtaa cggtaatccg atacggtggc
     5581 gtattcccct gtagcagccg atatcctgta agcgttttat attcaactgg acctctgatc
     5641 taagctcacc ttcaaggcga aattctccgg caatacgcct gatttcagca atctgggcat
     5701 cgttccagtc atcagctttg atatctaaat cgataccagc tcttgttaaa atttcgcggg
     5761 ctctgctccg gccaatacca taaatgtaag tgagggctac ttcacctctt tttttacttg
     5821 gaatatctat accagcaatt cttgccattt cttaattata ttaatttgtg tttaaccttg
     5881 acgttgtttg tacttggggt tctttttatt gatcacataa attctgcctt tacgccttac
     5941 aattttgcaa tcggcagatc tttttttcac ggatgttctg attttcataa ttcacaatat
     6001 ttttatttat acctaaatgt aattctaccc tttgttaaat cgtagggcga catttcaact
     6061 ctcactttat cgccggggag aattcttata tagtgcattc tcatttttcc cgaaatgtga
     6121 gcagtaatta catgtccgtt ctgcaattca actctaaaca tagcattcga taatgcttct
     6181 gtaattgttc cgtcttgttc tatcgaggct tgtttagcca taaattttat ttactctctc
     6241 tgttaagttc tttttcaatt atttcaaaat tcgataatat ctcagccttg tcttttttaa
     6301 cgattaccgt atgctcaaaa tgagcgctgg gttttccgtc tgccgttttt attgtccatc
     6361 catcgtgcat ttgcttcact tcttttttac ccatgtttat cataggttca attgcaattg
     6421 ccatattttc aaccagttta attccttgtc cgcttttccc ataattgggt acctcgggtt
     6481 tttcgtgaag ctttctgcca attccatgcc caacaagctc tcttacaacc gaaaaaccga
     6541 atgattcggt ataagtctga atagcgtgac caatatcacc cactctttta cctgcaactg
     6601 cttgtgcaat accgaggtac aaggcttttt tagtaacttc cagcaatttg tctatttctt
     6661 tgctcacttt accaactgtg aaagtgtagg ctgaatcacc atgaaacccg tttttgtaaa
     6721 ctccgcaatc aaccgaaacg atgtcaccat atttaagggg aacatcgttg gggattccgt
     6781 gcacaaccac atcgtttacc gaaatgcaaa gcgatttagg gtaaccgttg taattgagaa
     6841 aacccggtac agcaccatta tctctgataa attcatttgc gattttatcg agataatttg
     6901 aagttacacc ttcctttatt tcgcgggcaa gcagtgcgtg tgttttcgac actaacaggt
     6961 tgctttccct catcaggaca atttcttcgg ctgttttaat aaatatcatc aaagaacgta
     7021 taaaacccta cattgcagaa gtataacttc ctctgccttt aatccttcct gactttgtca
     7081 aaccatcgta atgacgcatc aaaaggtggc tttctatttg ttgcaatgtg tctaaaacca
     7141 cacctactaa aatcaataaa gaagtccctc cataaaaacc ggcaaactga ttgttgattc
     7201 cggccagcat tgcaatggat ggcaaaattg caataattgc aaggaagata gaaccgggca
     7261 gggtgatcct cgacattata ttatcgagaa aatcaactgt tttcttaccg ggttttattc
     7321 cggggataaa accgccgttc tttttcatat cttcagccat ctgttgtggg ttaatggtaa
     7381 cagcagtata aaagtaggta aatacaatga taagaactgc aaaaacgaaa ttataccaga
     7441 atcctgtata atcggaaaat gcagcaacaa atccactggt ggtactgcta aaaccggcaa
     7501 acatcagagg aacaaacatc aatgcctgag caaaaatgat cggcattaca ccggcagcat
     7561 taatttttaa ggggatatac tgccgaacac cgccatattg tttattacca acaacccttt
     7621 ttgcatattg tactggaatt cttcgtactg cctgaaccaa agcaatggaa agcataaata
     7681 caacaaacaa tgcaaccatt tctacgagaa acattactaa accaccggtt tgctcttcaa
     7741 ggcgggaaac aaattctgct gcgaatgaaa aaggtaatct tgatattata cctatcataa
     7801 ttatcaatga aataccattg ccaatacccc tatcggtgat tttttcgcca agccacatta
     7861 caaacattgt tccggctgtg agtattacta ctgaagaaaa cttaaacata aatcccgtat
     7921 acagaaatgc tgtggcggga agttgtgaat tcagattggc aatatatgta ggtgcctgaa
     7981 gcaataatat tgcaaccgta aggtagcgtg taatctgatt cattttcttt ctaccacttt
     8041 caccttcccg ctgaagtttc tggaagtaag gaattgcaat acccaacagc tgtactacga
     8101 ttgacgctga aatgtatggc ataattccca atccaaaaac agatgcgtta gaaaacgcac
     8161 cacctgaaaa catatttaac aaactcagaa caccatcggc agtctgagct ttcaggttgg
     8221 taagctggct cgggtcgata cctgggagaa cgatatagga acctaaacga taaattgcaa
     8281 tatatcctaa agtaaggagt atacgccccc tcaggtcctc aatcttccag atattcttaa
     8341 gtgtttctat aaatcgtttc atcctttaaa ttttttcagc gattccacca agagcttcaa
     8401 ttttctgaac agcagctttc gaaaaagcat gcgcttttac actaactttt atggtaaggt
     8461 taccattacc aagtattttt actttgtctt tttttgcaac aagaccattt tcgagcaaca
     8521 aaccatagtc gattgaatcg attttacatt tttcagcaag atattgtaat gtactaaggt
     8581 tgatggcctt atactcaaca cggttcatat ttttaaatcc gaactttggg gaagtccttt
     8641 gcaatggcat ttgccctcct tcaaaaccgc gggttttctt atacccggaa cgggattgag
     8701 cacctttatg tccacgtgtt gaagtaccgc ccttaccaga tccctgacca cgaccgattc
     8761 tttttttatt ctttacggaa ccttttgcag gttttaagct acttaaatcc ataactaatt
     8821 cccttcttta aattaaatat tttcaacttt caccagatga tgcactgctt taaccatgcc
     8881 taaaatttgg ggagtggcct catgttctac agtttgatgc atttttttaa tccccagtgc
     8941 aatcaatgtg tttttctgat ttggcggaca accaattttg cttcttactt gtgttatttt
     9001 aatttttgcc atgtttaaaa acttaaccgt taaaaacttt ttcaatgtta atttgtctat
     9061 gctgggcgac cgtattggca tcgcgcattt caatgagagc ggcaatggta gccttcacaa
     9121 ggttgtgtgg gttcgaagag ccttttgatt tagccaaaac gtcggtaacc ccaacacttt
     9181 ccagcacagc tctcatagca ccaccggctt taactcccgt tcctgaagaa gcgggtttca
     9241 gccaaacctt tgcgccaccg aattttgcta cctgctcatg aggaaccgtt cctttaacaa
     9301 ccggaacttt gataaggttc tttttagcat cttctactcc ttttgcaata gcagcggtaa
     9361 cttcgcttgc tttacccaaa ccgtaaccaa cgattccgtt ttcgtttcca actacaacaa
     9421 tagccgaaaa actaaaagta cgtccaccct tggtaacttt tgtaactctc tgtatcccta
     9481 ccagtctgtc tttcagttca agatcactgg tttttacttt tctaatgcta ctcgacatat
     9541 tattgactta aaattttaaa ccaccttctc tggcgccttc ggccaaagat ttaacacgac
     9601 catgatataa ataaccattc cgatcgaaaa ctacctcagt aatgccagct tcaatggctt
     9661 tttgggcaat tgccttacca accattttag cctttattgt tttatttgca tcctgctcag
     9721 caatgttttt aatcattgag gttgatgccg caagcgtaac accatttata tcgtcaatca
     9781 gctgtacact gatttgcttg ttactgcgat aaaccgtcat tctgggcctt ataggcgtac
     9841 cattaacatg tttccggata cggtttttaa tttttattct tctgttctgc tttgaaaaag
     9901 ccattgtaat aaattttact tgttaaacac tagctgattt tcctgacttt ctgcgcaatt
     9961 cttcattaac gaacttaata cctttacctt tataaggttc cggtttgcgg aatgaacgta
    10021 ttttggcagc cacctgaccc aacaattgct tgtcgtgact tttcagtgta ataatcgggt
    10081 tatttctctt ttctgttttg cattcaacct gtacttcttt gggcatcata aattgaatat
    10141 gatgtgaata gcccagtgag agttcaagaa tctgctcctt gctatcggct ttataaccca
    10201 ctcctacaag ttcctgctgt attgtaaaac cctgagaaac cccttcaatc atattggcaa
    10261 tcaaagccct gtacagtccg tgacttgcac gatcgttcct ttcttcggat ttgcgtttca
    10321 cgattacctc atttccttga acttcaactt cggtactatt gtgtattttt tgtgacagct
    10381 ctcccaaagg acctttcaca gtaacgatat tatccgaagt tgttatagta acatttgcgg
    10441 gtaattgtat tggtaatttt cctatccttg acatggcagt tcttttaata aatataacat
    10501 aaaacttctc cgcccacgtt tagctgacgg gcttctttat cggtcataat tcccttgttg
    10561 gtagataaaa tagcaatgcc aagtccattt aaaactcttg gcagctcgtt gctgggaaca
    10621 tattttctca accctggttt actgatacgg gtaagatttt tgatagcggg aatcttactt
    10681 accgggtggt atttaagagc tattttaata ctcccctgtg ggccatcttc ttcaaattta
    10741 taattcaaaa tatagccttt ctcgaaaagc actttggtca tttccttctt caggttcgac
    10801 gctggtattt ctactaccct gtgttttgcc attacggcat ttctgattct tgtcagaaaa
    10861 tctgcaattg gatctgtcat tgtatctgtc ttttttcttt accaactagc ttttttaact
    10921 cccggaatta gtcctttcga cgccatctca cgaaaaacga ttctgcttac gccgaactgc
    10981 ctcatgaaac cttttgggcg gcctgactga ctgcagcgat tatgcattct tacaggtgat
    11041 gcgtttttag gtatatcctg caatgcctga tattcgcctt ccttttttaa acgagcacgc
    11101 tttgcggcat atttcatcac aagtttttgg cgtttaactt cacgcgcttt catggattct
    11161 tttgccatat taattctttt ttaaattttt aaacgggaaa ccaaagtatt tcaataatgc
    11221 atacgcctct tcgtcgtttt tagtggaggt tacaaatgtg atatccatac ccataatttt
    11281 atagatttta tcgatatcta tttcgggaaa tatgatttgt tcactaattc ccaaggtgta
    11341 atttcctctg ccgtcgaatt tgtagttaac tcccttgaag tcgcggatac gtggtaatgc
    11401 aatggcgatg agtctttcga ggaattcata catcttttct ctgcgtaagg ttacagtaag
    11461 acctattggc attttcttcc tcagtttgaa attcgacacg tcttttttcg aaagagtggt
    11521 taccggtttt tgtcctgtaa tcatggtaag ctcttcaatt gcaatttcaa gaattttctt
    11581 atcggctatt gcttgtccaa ggccctgatt aataacaatt ttctccagtt ttggaatttg
    11641 cataactgat ttatatccaa attctttctt tagttcagga ataacttctt cctgatattt
    11701 tttcattagg ttaggcttat aatccatcac ttaatctcct ctcctgattt ttttgaatac
    11761 cgtactgact tttgattttt cccaattttg cgacctgtac gggttggctt tcctgtttta
    11821 gggtctgcga tcataagatt cgaaatatga acagcggctt ccttttttac aataccaccc
    11881 tgggtatttt ttgcattggg tttggtgtgc ttcgatatta agttcacacc ctcaacaata
    11941 gctctttcgt cctcgcggtt aacgctgaga acgcgacctt gctggccttt agattctccg
    12001 gctattacaa caaccgtatc tcctttttta acatgaaatt ttcttgacat ggaatatcgg
    12061 tattattttt ataaaacttc gggtgccagt gatacaattt tcatatattg tttttcgcgc
    12121 aactcgcggg caacgggtcc aaaaatacgt gtacctctga tttcgcctgc attgttaagc
    12181 aacacacatg cattgtcgtc gaagcgaatg taggaaccgt ccgagcgacg tacttctttt
    12241 ttagtacgta ctattacagc cttagaaacg gtaccctttt ttacgtctcc acccgggagg
    12301 gcagttttca ctgcaactac tatggtgtcg ccaatgctgg catacttatg ccttgtgcct
    12361 ccaagaaccc tgatacagag aacttctttt gcaccgctgt tatcggcaac tattaatctg
    12421 ctttcctgtt gtatcatgac tatttaacct tttcaacgat ttctactaat ctccagttct
    12481 tatttttact taaggggcga gtttccataa tacgaaccac atcgcctata tcacatgtgt
    12541 tttgttcgtc gtgagcaata aatttcttgg ttttgttcac gaacttaccg tatttggggt
    12601 gtttttcctt gcgttttaca gcaacaacaa tagatttttc cattttattg ctcacgacaa
    12661 caccaatacg ttcctttctt aagtttctgt tttccatatg caattgtgcc tattttttat
    12721 tttgtaattg cctctttctc aattcagtat ttaatctggc gatcgtgcgt ttcacctgta
    12781 ccaacttttg cggattgtca agcggagaca ccacatggtt cattttcagg cgaatcagcg
    12841 acgcttttgt ttcttctatt ctttcgatga tttccttttc ggataattct ctgatttcgg
    12901 tatttttcat tacagcaatt ttattcgtta aaattgtaat ctcttcttac aacaaatgag
    12961 gttttcaccg gaagcttctg agcagccagt cgcaatgcct cctttgccaa ttcaatagat
    13021 acaccatcgg cttcgaagag aattcttcca ggagtaactg gtgcaacaaa accttcagga
    13081 gcccctttcc cttttcccat acgtacctca gcaggttttt tggtgatcgg cttatccgga
    13141 aataccctaa tccaaatctg gccttctctc tgcatttttc gagttacagc aaccctggcg
    13201 gcttccagct gacgacctgt aatccatgct gattcgagcg attttatggc aaatgatcca
    13261 aaggctattt catgacccct ctgagcatta cctttcattt tgcctttctg ttgccttctg
    13321 tattgtgtct tttttggttg taacatcgct ataagtattt atacaattat ctatcttttt
    13381 gtatttctct gcttcttaat cacttttttt cctgcgcctt taacttcaat gcttggggat
    13441 aaatcgcgtt tgccataaac ctcacctctg catatccaaa ccttaacacc aatgagcccg
    13501 gttttggtaa gtgcttcacc caacgaaaaa tcaatatcag cacgaagggt atgcaatggt
    13561 gttctgccct ctttatacat ttcggaacgg gccatttcag caccgttaag tctgccagat
    13621 acctgcactt ttattccttc ggctcccatt ctaatggttg aggcaattgc cattttcaca
    13681 gcacgacgat aggcaacttt gccttcaatt tgccgagcaa tattgtttgc caccaaattt
    13741 gcatcgagtt cgggacgttt tatttcgaaa atattaattt gaacttcttt attggtaagc
    13801 ttcttgagtt cttctttcaa agtatccacc tcctgtccgc ctctaccgat aattattccc
    13861 ggacgggcag ttttaatggt aatggtaatc attttgagcg atctctcgat tacaatactc
    13921 gacacactgg ctttagccaa acgagcatta agataccttc tgatgctggc atcttcggct
    13981 aacttatcgg catactttct gccaccatac cagttggaat cccaaccttt aataatgcct
    14041 aatctattgg ctatcggatt taccttttgt cccatttaat ataaattaat tttcattttc
    14101 aactattgtt tcctgctctt cgtgttcctg ttcaaccaga ttacggctgc caataacaac
    14161 agtaacatgg ttcgaccttt ttctgatccg gtatgcgcgt ccttgtggtg caggctgcat
    14221 cctttttaat gagcggcctt cgtcaaccga aatctcttta atatataatc cggcttcttc
    14281 catacgcttt ccttcgtttt tagcctgcca gttagctact gccgacataa cgagtttttc
    14341 cagtttgcct gaagcatatt tgttggaata tttcaggata tctaaagcct tattcacgtc
    14401 ttcacctctt actaaatcgg caactaacct cattttgcgg ggtgatgtag ggcaattgtt
    14461 gagctcggca aaactaatgt tgcgtcgttc ctctttcatt ttttcagccc tgtttctttt
    14521 tctagcgccc ataatttatt caaatatttc gattattttt tcttaccacc atgacctcta
    14581 aaagttcttg tcggagcaaa ttcgcccagt ttatggccaa ccatattctc ggttatgtaa
    14641 acagggataa atttatttcc attgtgaacg gcaaaagtat gtcctacaaa atcgggtgaa
    14701 atcatactgg cacgcgacca cgttttaata accgacttct tgctccctga attcatagta
    14761 agcactttct tctcaagttt tacatcaata aagggtcctt tttttaatga tctgctcata
    14821 ctaattttga ttattttttg cgacgttcca caataaattt attcgatggt ttttttctgt
    14881 ctctggtttt gtagcctttt gccaacaatc cttttcttga tcttggatgt cctccggacg
    14941 cacgaccttc gcctccaccc atggggtgat caaccggatt catcacaacg cctctaacgc
    15001 gcggacgacg accaaaccaa cgggttcttc cggcttttcc gagtttctcg aggccatgat
    15061 ctgagttgga aacactgcca atagtagctt tacatgttgt gagtactttt ctcgtttcac
    15121 ccgagggcat tctgagaatt gcatatttcc cttcgcgcga agttaactgt ccataagtac
    15181 cagcactgcg ggccaatacg cccccctgtc ccggtctcat ttcaatgttg tgaacaattg
    15241 tacccagagg aatatcggat aaaaacaatg tgttccctat ttcaggagcc acgtttttac
    15301 cagtttccaa aacctgacct acgttaattc catttggcgc gatgatatat cgtttttcac
    15361 catcttcata acataccaaa gcaatgcggg cagtgcggtt tggatcgtat tcaatagttt
    15421 tcacaattgc tttcactcca tctctgtcgc gcttgtagtc aattattctg taacggcgtt
    15481 tgtgaccacc tcctatataa cgcatggtca ttcttccctg attattacgc ccgccggttt
    15541 tcttaatcgg ctcaagtagc gatttctcag gtactatatg ggtaatttcg tcgaaagtgc
    15601 tgataacttt gaaacgttgc cccggtgttg ttggttttaa ttttcttaca gccattgcta
    15661 ttaaatattg ctataaaaat caataacttc tccctctttt aatgtcacaa ctgctttctt
    15721 gaaggaattg gtcttacctt ttacaagccc tgtttttgtg aacctcgact tggctttacc
    15781 tccataacgc attgtgttta cttccataac cgaaactcca tacagcgttt caacggcagc
    15841 ttttatctga attttatttg cagttttgtc tacaataaaa ccatatctgt ggagcttgtc
    15901 ggattgcgcc atcatctttt cggtaactat tggtcttaaa attatttcca ttacaggtta
    15961 tttttaagct attattttct ccaaaacatc aatcgattct tgctcaaaca ttaaagttga
    16021 tgcccacata atatcgtaag tacttaaatc tgaggcagtt ataactttaa catttttcaa
    16081 atttcgagat gacaaatata tattattatt tggttccttc acaaccaaaa tcattttttt
    16141 attcgacaga ttgaaattgt taaccaaatc gataatattc cttgttttaa cgtcttcgaa
    16201 caagagatta tcaacaatca caattccatt gctgttgata cgatagctaa gtgccgattt
    16261 tcgggcaagt tttttaactt ttttgttcag tttgaaggaa taatctctgg gttgaggccc
    16321 aaaaacacgt ccgccgcctc tgaaaagtgg gttattcatt ccgccggcac gggcaccacc
    16381 ggtacctttt tgccttttca gttttcttgt acttgctgct atctcacttc tttcttttga
    16441 tttgtgagta ccttgtcggt tattggccat gtattgcttg catgcaaggt aaatagcatg
    16501 gtcgttaggc tcgatggcaa aaatagaatc attcagatct atttcattgc ccgtttcctt
    16561 tcctgatttg ttatatacgg gtaatttcat tatctttcaa taattaagta tgaaccaata
    16621 gctcccggaa cagaaccttt aatcaaaacc aggttgcttt gtgggataac ctttaacact
    16681 cttaagctta tcattttcac ttttttgccc cccatgcgtc cggccattct catgccttta
    16741 aaaaccctgg atggccaaga tgaagctccc agtgaacctg gtgctctgcc tctgttatgc
    16801 tgtccgtgag ttgcatcatt tacaccggag aatccatgac ggcgaaccac accctgaaat
    16861 ccttttcctt tagttatacc agaaacatcg cagaattcac cttcgataaa aatacccaca
    16921 tcgataatat cgccaacaac ggggtcgcga agttcgtcat tcactcttat tttgccgaat
    16981 tttttgaact caacaacctt tttcttggga gtagttcctg ctttggcaaa gtggccttgc
    17041 attgcttttg ttgtatgctt gtcctttttg tcgtcgaaag caagttgcac tgctatgtaa
    17101 ccatcctttt caatcgaacg gatctgggtt actacgcaag ggcctgcttc aacaacagtg
    17161 catgggatat ttttcccctc ggcactatag atggaagtca taccaatttt ctttccaata
    17221 attccttgca ttgttttatt ttttaagtat tacatcaaac tttaatttca acttcaacac
    17281 cgctgggtag ctcaagcttc attaaagcat cgattgtttt tgcagtcgtg ctgtaaatat
    17341 cgagcaacct tttataagag ctcaattcaa attgttcgcg agctttcttg tttacaaatg
    17401 ttgagcggtt tacagtaaaa attcttttgt gagttggcaa aggtatcgga ccacaaacaa
    17461 tggcaccggt tgcttttacc gtcttcacaa ttttttcggc tgatttgtca acgagattgt
    17521 gatcgtatga tttcagtttg attctgattt tttgacccat attcataagc ctgtcctcat
    17581 taattaaaat gtatatttat tagcattaat aatctcttct tgaattagcg ccggaactac
    17641 agcatagtga gaaaactcca tactaaagct cgccctaccc gacgtaattg tgcgtaatgc
    17701 agtaacataa ccaaattgtt cagccaacgg aacttcggct ttaataaccc tgaccattcc
    17761 tcttgaatcg aggcctgtaa ccgatgctct ccgacggtta aagtcggcaa ctacatcgcc
    17821 aacatattct tctggtgtta caacttcggt ttttgtaatg ggctccaata ttactgattt
    17881 taccttcctg ctggcttcgc tgaaagcttt tcttgcagca atttcaaacg ataaggcatc
    17941 agagtcaacc gaatgatacg atccgtccag taatgaagct ttcagactgt ttaaaggata
    18001 tcctgatatc gggccgtttt gcatggcttc tttcaatcct ttttctacag caggaataaa
    18061 ttctttcgga atacttccat ttttaataca gttttcaaac tgcagacctg taaaaccatt
    18121 ctgctgtatc ggttcaaatt ttagagtaag ttcagcaaat ttcccctttc caccggtttg
    18181 tttactgaat atcatgtgat attcaatcga ttctgtaagt tcttctttat agttaaccct
    18241 cggagctccc ttatttattt tcagtccgaa ctcacgtttc aacctatcgg tgatgatgtc
    18301 aagatgaagc tcacccattc cactgataag agtctgcccc gattgttcgt cgtatttcac
    18361 attgaaagta ggatcttctt cctgtagtct attcagagcc gaactgagtt tttcgatatc
    18421 agcctgcatt tcgggttcaa ctgccataag aattaccggc tcaggaaagt tcattgcttc
    18481 aaggctgatc ggcttgtttt catcgcaaat cgtgtcgcct gtttttacgt ctttcaggcc
    18541 tactactgct atgatgtcgc cggctttagc ttcgttgatg ggaagttgtt tgttggcgtg
    18601 catctgaaac aatctcgatg ccctatcttt tttaccggtt cttgaattat agatcatttg
    18661 accggagctg actttaccac tatatatcct aacgtatgta agttttgcga aaaacggatc
    18721 tgaggcaatt ttaaaacaca gtcctgcaaa tggttcgtcc tcagaggggt gccttacggc
    18781 ttcctgctca gtgcgaggat taataccagc taccgcgcta atatcagatg gtgaaggcag
    18841 gtatgcacaa attgcatcga gcagcaattg aacgcccttg ttcttaaacg atgacccgca
    18901 aagtacagga acaagttgtc cgctgataac ggctttcctc attgccagtt taaattcatc
    18961 gacactaacc tgtttgcgat catccagaaa tttttccaac aactggtcat cggaaatagc
    19021 cattacttta tcaatcatgg cttcacgcat ttcttcagca tgttccttca gattctcggg
    19081 aacatcttga atatcgaaat ttacgccgag cgaaccattg gtatcgtccc aaacgtatgc
    19141 tttatttgta attaaatcaa caactccgca aaaaccatct tcgaatccga taggaatttg
    19201 aactgccaca gcattagctc ccaacttttc gtttatctga gcaacaactg actgaaaatc
    19261 agatcccacc ctgtccattt tattaacaaa ggcaatcctt ggtacgtgat atctgtcggc
    19321 ttgatgccat acagtttctg actgaggttc aaccccgcct acaccgcaga atactgcaac
    19381 agccccgtcg agtactctaa gagaacgctc cacttcaaca gtaaaatcaa cgtgaccggg
    19441 tgtgtcaata atattaatgg tatatttgtt ttcgtcatac ttccaaaagg ctgtagtagc
    19501 agcagaagta atcgtaattc ctctttcctg ttcctgctgc atccaatcca tggtagctgc
    19561 accatcgtgt acttcaccaa tcttatagtt cacaccggta taatacagaa tacgctcagt
    19621 tgttgtggtt ttacccgcgt caatatgagc cataatacca atgtttctga tatgtgaaag
    19681 tacttcaggc atgggagtat ttaaaatctg aagtgtgaga atgccttatt agcatctgcc
    19741 atacggtggg tatcttcctt cttcttaaaa gcagctcctt cgttgttgta agcagcaatg
    19801 atttcagcag ccagtttctc tgccatacct cttccactcc gtttacgggc aaagcctatc
    19861 atccacttat ggctaattga aattttgcgg tcttccctaa tttcggtagg aacctgaaac
    19921 gttgctccac caattctacg gctttttacc tcgacctgtg gggtaatatt atcaagtgct
    19981 tttttccaga tatcaagggg tgcatcttct gagtccttca ttttcttctc aacaatatca
    20041 agggattcgt aaaatatctc aaacgatgtc gactttttac cactccacat catatcgtta
    20101 acaaaacgtg tgaccctaac gtcattatat ttagggtctg gcaatatcaa tcttttcttt
    20161 ggtctagttt ttctcatacc ttattatata taagctatta attatttctt ttttgctgct
    20221 gcagcttttt tctttttggc tccatatttt gaacgacgct gcttacggcc gtcaacacca
    20281 gcagtgtcga gagcacctct tacaacgtga tacctaacac ccggaagatc tttaacccta
    20341 ccacctctta ccagaacaat ggagtgctct tgcaaattat ggccttcacc cggaatataa
    20401 gcattcacct ctttaccatt tgttaaacgc actctggcta ccttacgcat agcagagttc
    20461 ggtttttttg gagtcgtagt gtaaacacgt gtacacactc ccctacgctg tggacatgaa
    20521 tctaaagcag gagctttact tttgtctatc aaagccagcc tgccttttct tactaactgt
    20581 tgtattgttg gcatacaaac actcttttaa taatttaaaa atttctgttt acaaattgga
    20641 ctgcaaaggt agattaattt ttatttttaa caataaaaca tgaaaatttt attgtttttt
    20701 tctgttagca gtatttttag ccgttttcga gttgttaata aaatgattaa atcgagtgta
    20761 ttcccttttt ttcggcataa ttttagatta tcaacctaaa aattcgaaaa gacatttgct
    20821 tattaattcg atttatgaat tacttttgtt gtaataaaat aacactacta tgaaatttct
    20881 cactgtacca atagtatccg ctcttttttg ctgcctgcta cttataaatt catgcattga
    20941 accggttcct ccttccgata agcttgaagt tgtattggtg tcatcaaata ttacaacccc
    21001 gacaacgtgg gcaggcgata aggcttacgt tatccagagc tccgacttct atgttgaggc
    21061 agcattaacc atagaaccgg gtgctgttgt aaagttttcg ggacaaaatg cttatctcac
    21121 acttggggcg gagggagcta tcaatgctgt tggcaccagc gaaaaaccta ttattttcac
    21181 atcactgtat gacgatgagc acggagggga ttccaacgat gacagcgatg ccacttcacc
    21241 acaggctaaa gattggggta ccatcgatct gaatggtacg caaggatcgg tattcaacta
    21301 ttgtgaattt cgttatggtg gtagaggtgc tacgcctgaa ccaactctga atctttcggc
    21361 agacgcaagt gcctctgtta caaattgcat gttcatcgac aatggaggtg gtatacggac
    21421 atcaggaaac ctaaccagtt atattggtgt tgtgcacgct gaaagtgcca ataatcaaac
    21481 aataattcgc aataacctgt tttatagaaa tgttgttcca ttggtgattt tcgcagaaat
    21541 cagtatcgac aattcgaatt cattttcgta ctatgcaaat tcaaacacaa tgaatggtat
    21601 ttttgttagc ggagttacaa tgatgaaaac caccagctgg cttgaaactg aagttgccta
    21661 tgtaattacc tcaggcgacc ttagcattaa cgaaccttat aaacttgtac tgggcaacga
    21721 tgttgtcctg aaatttgtga acgacagccg cctgaattgc ggtgggggag aaatagctct
    21781 ggaaaatcat agtggcgacg gagtattctt tacttcttat aaagacgaca attacaaagg
    21841 cgattcaaac ggtgatggag caaattcatc gccagccgaa gccgactgga taggaatttt
    21901 tcttgatgac tataaaggtg ggtttgccga ctggccaaac atcctctaca atgatccgga
    21961 tgcattggtt aaaagcacag gggtaaatta actgaaccag tttctattca aatcaatatt
    22021 tccacctgat agaactatac aagtggtttt attttcaaat attcctttat tttcaagaac
    22081 acacgctaat ggcactgcag acgagggttc tataattatt ttcattcttt cccatattag
    22141 tctcatagcc tcataaatac ttttttccct gcaagtaaga atatcgtcaa cgaaattgag
    22201 aattagcgca aaatttcttt ccccaagcga agtaagtaag ccatcggcaa ttgtatctgg
    22261 gttttctgac ggaaaccatt ttttggcata gaaagagcga aaagcatcgt tggcttgaat
    22321 tggttccgca gctataaccc aaatttttct attcaagctt tttgctgcaa ccgcagttcc
    22381 actaattagt ccacctccgc caaccggggt catcacaatg tcgatatttt gctccgaacg
    22441 cattacctca gcaattgccg tgccctgccc cgacataata atataatcat cgtaaggatg
    22501 aataaaagct gctcctgttg catcgattac ttcacgggcc gtgctttctc tcgacataag
    22561 gttgggttca caaaacctca cattcccaga atatgctttc acagcttcaa cttttacaca
    22621 tgatgaagtt tttggcataa ctatatgggc ctgaataccc ctataagatg ctgcaagggc
    22681 caaagcctga ccaaaatttc ctgaagagtg ggtacacacg ccattttttg cttgtaattc
    22741 ggacaagctg aacacagcat tgcaggcccc gcgaatttta aaagaacccg ctttctgaaa
    22801 attctcacac ttaaacaata tcgttccacc caccattgaa ttaatggttt tattcgataa
    22861 aatcggcgta acgtgaatgt agggtgcaat tcttcgtgaa gcttctgcta ttgtgttcaa
    22921 atcaatcatg ataactcggg tataaaagct tcggggaaat gagtaatttt gacgtcacca
    22981 tttgcattta aaacaacaga aacaacatcg aatcgggctt cagcgtcatc aggataattt
    23041 gccatatagt cgcccgcagc ttcaattaag agtttttgct ttcttgcatc aaccgattct
    23101 tcaggtcgct gccactgatc agaactcctt gtttttactt ccacaaacac aatgagtcca
    23161 ttttttctag caataatatc aacttcacgg tgctgtgagt gccagtttgt actaagtatt
    23221 tcgaatccat ggctgattaa atactgcgct gccagcattt cgcctttatt ccctgtgtcg
    23281 ttgtgattgg ccataaaaaa acccggagtt accaggttta ttcattatta cattttttac
    23341 ttcttcgtaa gcttacttgt tttcatgagg attccatcgg aatataatct cacaaaatat
    23401 acaccttttt cccaagtgga acaatccaca actaaattat tgttgttttg aaatttttta
    23461 tcaagaattt ttcgtccgat catattatat acctcaacct gaagcttagc gccttcaaca
    23521 tcggcagcat cgatatagaa aaaaccgtca gccggattcg gataaaccga tgttgcgctt
    23581 agggtgttat agtttaccga actgctatta acaacaaagt caacattaac atatgattca
    23641 ggccctcctc ctgaaacaaa aaagtaatat ctcatcgaag ttgtacctga attaccctga
    23701 caatggtaat gggccgtaaa tttgtgagct tcatcagtag ttgctcctgc tgccaaagta
    23761 taaggaggtg ccgaaaaagt gttgctggga taacatgtag tgttccagca aatatcgctc
    23821 attgtattgg gtataactga aatctcttct ttccgacatc tgacatcaag acttgcagaa
    23881 gtgttgttct gaatataaaa atatattatc gcagaattaa gatttaacgc gatattgccc
    23941 tgcacggtaa tagtttgacc gttcactata ttgccatcat gatcttttat taaaatttcg
    24001 ctctgggcaa gcaaaattac atttgcaaaa attataagta aacttgtaaa cgcaattttc
    24061 atatgttact ttttttacaa atgtaaggca tattttcgct caaagaatca atcattgaaa
    24121 tttttataca tttttaacgg tattttaata aaatcgtata aaaaccagtc agagaatagc
    24181 aacttattca aaacataatt aatattgtat cttaatatta actaaaacag aataatttat
    24241 gaaacacatc tttttattta ccattttatt ggggctattt tcggttgcat ttgcgcaaca
    24301 tacaattgaa atagaactga aaaccgataa ttatggcagc gaaaccactt gggaagttgt
    24361 taacactttg aacaatgatg ttctggctga aggaggccct tacaccaatg tttcagcagt
    24421 gcagactatt gcacccattg atgttgacgg aaccggctgc tatgcctttt acatttacga
    24481 tgaatatggc gatggcatct gctgcaatta cgggtcgggt tattacaaag tttattacga
    24541 cgatgttatg attggccaag gcggtgaatt ttccagtgag gcatcggtta tgggtatggg
    24601 ctcgggctgt ccgcagaatg aaattgagct cattgcaaat aacacacttc cctggactgc
    24661 acgtcaaggc gattatgctg ttaccggaca aaacacaaat ataaaaggtc gcataattaa
    24721 caacggtgta gttaccctga cttcgtttca ggttacatac aacattgatg gtggagaaaa
    24781 tcttgctccc caaacaatca cctgcaatgc aaccatggga cagacggtta attttacata
    24841 taatcttaat cctgctgttt tcagcaccga tggaccttat gttgtaaaca ttatcgtttc
    24901 taacccaaat ggcgttgagg atgacgacag cgacaatacc agttcagtaa atattattgc
    24961 caattcaaat tcagtttcaa gaaatgtgtt actggagcat tttacaactg ctgaatgccc
    25021 taattgccct gcagctatta ccaatcttac gaactggacc aacaccagac caaatattat
    25081 ttggttgtcg catcacgcag gctactatac cgatgatctg acaataccgg aaaacactca
    25141 gctgcttctt ttctataatg cagggggatc cacctatgct cctggcatta tgctcgacag
    25201 aaaattcctt tcacctgatg gagatccggg ccctgtattt ttcccggcaa gttcctatac
    25261 aaccggctta atggatcaaa tgagaaatga accagcatat gtcagtctcg aaatggaagg
    25321 taattacaat accgaaacag gattgctaaa tttaaccgta tcaggcgagt ttttaggcac
    25381 agttgccgga acaccccgtc tgagcgttta cattcaggaa gacggactcg ttggcgagca
    25441 ggcaggagca tcgaataatt acactcacaa ccattcaatg agagatgcta ttacagcaaa
    25501 ctatggtgat gccacagcta ttccaaatgg gaacaccggc acaacattca ctaaaaccta
    25561 tacctatact gttgacagtg aatgggtgct tggaaatctg acattaattg cttttgttaa
    25621 tgattggaat aactcaaatg tgaacgaccg tgagatttac aactccatta aaatgccatt
    25681 gagcgatttt gtatcaattg cagatgaata cagtgaaaac attatgattt tcccaaatcc
    25741 ggcaagtgat atgattacaa ttacaggtgt tgaaaatgcc agcgtcaata ttttcagtgt
    25801 tacaggacaa cttgttttct ctgacaattg caatagcaac gaaattactg taaccaccgc
    25861 caatctgtcg gaaggaacct atattgttaa agttgttaaa gattcttcta ctattgtgaa
    25921 aaaagtaata gtaacaaaat aaattttgaa agaaaggggg cttctacccc ctttttttta
    25981 atttggtaaa gtatttgtta ctacggtgaa gaaattttaa aacattcatt atgaaaaagc
    26041 tattttttgt atttgttttg gttgtaatgg caggatatat ttttggtcag gaagtacctc
    26101 agtttcagat taaagatata aataataaaa ctgtaaacac gaaagatatt attaagaaca
    26161 acgggaaacc ggtcgtaatt tccttttggg ccacatggtg caaaccctgt ataaaagaac
    26221 tgaatgcata caacgaaaac tacgccgatt ggcaaaaaga aaccggtgtt aaaatcattg
    26281 ccatttcgat agacgactca cgaagtatga gtaaggtggc tccattcgta aatggcagga
    26341 tgtgggaatt caatgtgtac tgcgatccaa acagcgattt caagcggggt atgaacgtta
    26401 acgaagttcc gcataccttt gttctggata aaaacggaaa agtcgtttgg caacacaaag
    26461 cctatgtcga aggggacgaa aacacccttc acgaagtaat acagaaagtc gcagcgggca
    26521 aaccattaaa gtaaattatt gactcaaata attattatgt tcagaaaact atgttttgta
    26581 atggcaatag ctttattgcc attttcgttt gtatgggcac aggagggtac atgggggcaa
    26641 atcacaggaa acttccaggc cgacggacag tattatattg ctgattctat aattggcgca
    26701 tcgagtcctg cagaaaaaac catgctgaat tcctttgcca atttcaccta taacctgggt
    26761 aatttctcag ccggattccg ctacgaagga tatttgaata ccctgcttgg ctatcctaat
    26821 cagggaggaa ttaacgatgg cattgggata ccgtaccgat gggctaactt tgtaaacgac
    26881 aagttcgaga ttactgttgg caatttttac gaacaattcg ggaacggact tgtattaaga
    26941 acttacgaag aaaagcagct aggcatcgac aacgcactcg atggtatcag agtaaaaatt
    27001 agtccctata agggaattta tcttaaaggc cttgtcggga aacagcggct ttactgggag
    27061 aaaggtgaag gtattgttag agggttcgat ggtgatttta tggtaaatga aatcgtacct
    27121 gaatggaacg aaaaaccatg gaatcttgga tttggaggaa gttttgtaag caaataccaa
    27181 gcggatgagg atcctgtgta tgcactacca caaaatgtgg gagccggggc aggaaggctt
    27241 cttttttcgg ttgccggttt gagtataaat tcagaatatg cgtacaaaat aaacgatcct
    27301 tcttccgata atgggtatat tttcagacct gggcaggctt tcctggtaaa tgcgagctat
    27361 tcacgtagcg ggttaggaat tcttgtagcc accaagtgga ttgataacat gagctttcgt
    27421 tccgaccgca atgctatcag taccaatctg aacataaacc aattacctga aataacaaaa
    27481 gcccacacgt actcattaac tgctttttac ccttatgcaa cccagccaaa cggagaatgg
    27541 ggcgtacagg cagatgttat ctacaagttt aaaaagaata ccaaaattgg tggtaaatac
    27601 ggcacttcaa tcgctctgaa tttttccagg gttcaaaata cagcccaaac gcctgtgaac
    27661 gattcaacgc caattggaag tcatggcacc tacggataca ataccaattt ctttagtatt
    27721 ggagactccc tttactttca ggacatcaac tttgaattgt cgaaaaaatg gagttcaaaa
    27781 tttaaaacca ttttcaccta ccaaaacatc atatataatt ataatgtact cagaggaaca
    27841 agaggtcatg aaactgtatt tgcccattgc gggattgccg atataagcta caacttcact
    27901 gataacatat cgcttcatac cgaattgcaa gcccttttca caaaacagga taagggaaat
    27961 tggggtatgc ttcttttaga atttactatt ccccattggt tttttacaat ctccgataac
    28021 tggaattatg gaaatagtga tgcctcgcac cgcacacact atcttttggc cggttttggt
    28081 tataacaatg gcgggaacag aattcagctg agctatggga aacaacgcga aggaataaca
    28141 tgcgtcggcg gtgtttgcag ggctgtgccc gcttcaaatg gtataattat cagtatttca
    28201 agtacttttt aaaaaacctg ccatgaaaaa attattcatc ataacatttt gcctgcttgc
    28261 aagcgcattt tttacaacct gcgatatcat agaggcgcct tatctcgaca caaacgagcc
    28321 ggtctggaac gggcgaaaaa gcctaattct cgatttcaca ggtcatatgt gtgggaattg
    28381 cccttacgca caccgtacta ttgtttcaat gcaaaataat tttggcgaag cagttgtacc
    28441 tattgcagta cactgcggtt atttcagcat gttagcatcg aacgatccgt tgcagcccta
    28501 ccactatgat ttcactaccg atgtaggact tgagctggga ggcaatggtt ttacttcgta
    28561 tggatatttt ggcgttcaaa gtcaacctat aggagtagta aaccatttga ttccggaatc
    28621 gctgaaagct cacgatgggt gggggtctga aatagctaag tactatagct cattccctga
    28681 attgatgatt cacgttgata aaactttcaa cgaaaccgac agcactttaa tagcagttgt
    28741 gcatgttgaa agccaaatag aatcaacccg aaatttatcg cttgctgttt atattaccga
    28801 aagtcatatc attcagtggc agaccgacta ttcgcaaacc gattcaaacg ttgaagacta
    28861 tgaacacaat catgttctcc ggggaagttt taacggagcc tggggagatg ccataaatac
    28921 aaacaataat ccaataaccc gactacagca atttgaaaaa gagtactcct taaaaacagg
    28981 cctcgattgg gttcctgcaa accttagtat tattgcattt gtttacgaca ccgacagtaa
    29041 agaggtgctt cagtgtgaag aaattcatgt tggcgaataa tgagatcgtt ttttttaata
    29101 ttgttcttca cgcttataag tctcactcag gcatatgccc agcaattcta tgccggtgcc
    29161 attggcggac tggttttatc gcagattgat ggagattctt acggaggcta tcataaattt
    29221 gccggaagcg gtgggctgta tgtaagaaac aactttggcg aaaaatgggg atatcgcatg
    29281 gagctaaaat acatccagaa aggaagcaaa gccgcacgcc ttgatatggg atatcatact
    29341 ataccttttt acaaagcatc tttgaattat atcgaaattc cgtttctttt ttcgtatcgt
    29401 atgcaaaagt tcaaaatacc acccactatc gattggcaac tcgacggcaa actacatttg
    29461 aatattggta tgaatctgag ttatctattc agtgccaccg aagacgatgg gactggtcct
    29521 aatgaagcag gctttgcatt ccgccgtata gaattggggg ctcaaaccgg agttacttat
    29581 tattttagtc ctcactgggc ttttgacttt cgtttctgtt acactattat tcccatacgc
    29641 gatcgcccca acgcctcgga ccttgattat tgggtaacct acgagttcaa ccgcgttctg
    29701 ttgttttcga taatgtacga aatatgaaaa caaaaaaatc cttggtcatt gctattacag
    29761 gtgccagtgg tagcatttat gcaagcacac tgatatctga tttgctttta ttgagagatt
    29821 atatcgaccc tgagaggctt tcgttggtag tttccgacaa cggagatcag gtttttgaac
    29881 atgaaatggg atatcgggct tctgcaataa gtggaattaa gcactattca aatactgatt
    29941 tctacgcgcc gtttgcatcc ggatcgggta attacgatgg tatggttata gtgccctgta
    30001 gcatggggac attaggcaaa atagcacatg gcatttctga caatctgata accagaaccg
    30061 cagatgtaat gcttaaagaa aggaataaac tgattttggt aacacgcgaa accccgatca
    30121 gtctcattca tattaagaac atggaattaa ttaccttggc gggaggtatt gtttgtccgg
    30181 caaatccctc attttacagt aagcctttgc aagtacacga tttggtcaaa accgttacca
    30241 accgcattat ttcgctgctc ggtattgaat gtaattcgtt tcaatggcct ggaactttta
    30301 cgccttagcc actaccgcaa tacttactgt aactccttcc acacccagta gtgtttcaac
    30361 caccgcttca atggttgatc ccgtagtaat tacatcgtca acaaccaaaa catgttttcc
    30421 tttcaggttt tcagggcttg ctatctggaa tttaccgctc acatttttcc accgttcgtc
    30481 acgtgttttt tttgtttggg tttcggttgc ctgacttctg attaattcat ggagtttaac
    30541 aggaattcct gtaacctgcg aaatgcccaa tgcaatttcg gcgctctgat tatatcctct
    30601 ttttgtttgc cgtgatatgt gaagcggaac agggataatg caatcgatgc ctttaaaata
    30661 atcagacttg ccgatacatg cacccagttc ccttcctaaa aaaataccaa tttctcgttt
    30721 tcctttgtat ttgagcttat gaataagttt acggtatttg cttccttttg caaaatagaa
    30781 tagtgctgtt gccatttcga cgtgtacttt tccccaaaaa atctgactta ccgggttttc
    30841 gggtttgttg tgataatcgg tgtatggcat gtcggcaagg catttcaaac aaatagtttt
    30901 ttcacccgtt acaaggggag ccatacaaac ctcgcaaacc tccggaaaaa gaaggtagac
    30961 caaatcgcag gcccactttt ctatattttt caaaatgctt gacattactg taaaaataca
    31021 aaaataaaac tgtctttatg gaatttgatt ttgtttcaca tccaaaactt gaaaaaaaca
    31081 tacaaacaat taaaaataaa agctatgaaa aaaatgattt tattaatggt catcggagca
    31141 gttttaaccg gctccatcca tgcagcaaca gtttccggaa taattaccga tcttaccacg
    31201 ggtaccacaa ttggaaatgc tcctgtggta attgcaaaaa ccaatgggtt aagcctgaaa
    31261 atgactcagt cggacgcatt cggttatttt gaattcaaag atttatcggc tggcgactac
    31321 cttatcaaag tgaattacga aaactatgaa gaatatgaac tcgcctttac gatggcagag
    31381 gaagaaaata aaaatttgtt cgtttacctt tcggttcagc caagcttaac agatgtactt
    31441 tttaataata aagatattga agagcacaaa caagattgtt caaaaacctt atactcaaga
    31501 gaatcttcag cagctccttg cggaggtgcc gggtataact catttgccta cgacgaaccg
    31561 gcaatcgact ggaataccga atcgtatgac tatattaacg aaaacaaatt taaaacggtg
    31621 accaacgatc ctctgagcac cttttcaatt gatgtcgaca gagcttcgta ctctaatgta
    31681 agaagattca ttacacagaa ccaaatgcct tatgctgatg cagtcagggt tgaagaattg
    31741 ataaattatt tcgattatac ctacaagcaa cccacaggag aacatccatt ttccacaaca
    31801 ttagagctgg gtacatgtcc atggaataaa gaccatcagt tgctactaat aggaattcag
    31861 gggaagaaaa tgatctcgga agaaatacct gcaaacaatt tggtattcct gattgatgta
    31921 agtggttcta tgagcgacat taataagctt ccgctgttaa aacaatcatt caaaatactt
    31981 atcgatcagc ttcgtccgca agacagagta gccattgtag tttatgcggg tgccgccggt
    32041 caggtacttg cctcaacatc cggaaaagac aaagctaaaa tatcagccgc acttgatttg
    32101 ctggaagcag gtggatctac cgcaggtgga gccggaatta atcttgcata taaaattgcc
    32161 aaagagaact atattcccaa tggaaacaac agggtaattc ttgctaccga tggcgatttt
    32221 aatgttgggg ccagcagcga cggtgaaatg gaaaggttaa ttgaacaaaa gcgcaacgac
    32281 ggtatttttc tcactattct tggctttggt atgggaaatt acaaagactc caaaatggaa
    32341 aagcttagca acaaaggaaa cggaaactac gcatatatcg ataatataat ggaagcaaaa
    32401 aaaatattca gtgaagaact gtggggcact cttttcacca ttgctaagga tgtgaagata
    32461 caaattgaat tcaatccttc taaagtgaaa gcatatcgtt tgattggtta tgaaaaccgc
    32521 cttttgaata aagaagattt taacgatgat accaaagatg ccggagaaat tggaagcgga
    32581 cacagcgtaa cagcactcta cgaaatcatc cccggagaat cgggggaacc gctggccagc
    32641 gttgatccgt tgacttatca ggatgttaac attcgcaaaa gtaatgattt gatgacacta
    32701 aaaattcggt ataaagaccc taagggaacc gaaagcaagc tgataacatc gaaggtagca
    32761 tcaaccgatc tcggaaaaat atccgataac tttaagcttg ctgcatcgct tgctgaattt
    32821 ggtatgttgc ttcgcaattc tgaatttaaa ggaacggcaa cttacgagga tgcaatcaga
    32881 cttgctaaag aggctatggg gaacgaccct tacggataca gggccgaata cgttatcctt
    32941 cttgaaaaga caaaactcct gagcaaataa aaaagtcagt aaaataaaag accacatttt
    33001 attaaataaa gtgtggtttt ttctttggtt tttaaaatta ttgttatttt tgcagaccta
    33061 aaaaaaatcg ttctttcttg tccgatggtg taatggtagc actgcagatt ttggttctgc
    33121 ctgtccaggt tcgaatccta gtcggacaac acatattgac ctgtggtgta attggcaaca
    33181 cacaagattc tggatcttgt tttttaggtt cgagtcctaa caggtcaaca acaaaaaatc
    33241 cgtttattag aacggatttt ttgttatttc taataccctc ctgtgatatc ccagacaaat
    33301 gcacggatac ccacatcttt gtttatatga tcaaggtttt tcaccttttg gaggattttc
    33361 tcgacctgat cgtcttcgac aatggttaat atggcagaat taatttcggg ccatgtgtgg
    33421 ttgccatagt gaggatctcc ggtttcgctt cccctgccct gcatgttcga ccattgagaa
    33481 tagcctctga ttcccagcac gtcaatcatg tattccacct tgtcggtgag ggccatatta
    33541 tagacaatga atattgcttt catagtatta ttcttttatt tctttaacat cgttggtatc
    33601 ttccataaaa ctatacttca gtcggtgaat gtttcgtttt ctcagttttt cgcctttacg
    33661 tgtaaacaaa gcgtaaagaa ccggcacaat aatcatggta ataattgtag aaaataccaa
    33721 tccacccata accgaaatac ccatcgggct ccaaatttct gacccttcac cggtgctcat
    33781 ggccattggc agaagtccca aaatagtcgt aagggctgtc atcagcaccg gacgtaatct
    33841 ggacttgcct gacagagcaa tggcatcata caggtcgtat tccctgtcgc gcatcaggtt
    33901 gatatagtca accaacacga tggcattctt caccacaata ccgataagca acacagcacc
    33961 caacgcagca ataacactca gggttgttcc cgtaatatac agggcgagaa taacccccga
    34021 gaacgagaat ggaatt
//
LOCUS       DAOOQP010000018        32493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1581_length_32493_cov_6.540046, whole genome shotgun sequence.
ACCESSION   DAOOQP010000018 DAOOQP010000000
VERSION     DAOOQP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1581_length_32493_cov_6.540046"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..892
                     /gene="pepE"
                     /locus_tag="PLK75_03435"
     CDS             182..892
                     /gene="pepE"
                     /locus_tag="PLK75_03435"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOY38328.1"
                     /translation="MQLLLISNSTMAGEAYLDYPKYDIRDFLGNKPVKALFIPYAGVT
                     VSWDDYEAKVKNRFNEVGHDVISIHRFSDPVKAVEEAEAIVVGGGNTWNLLRKIHDNK
                     LLEPIRSKVLSGTKFIGWSAGSNVACPTIMTTNDMPIIDPIGFDACNLVPFQINPHYL
                     DANPEGHGGETREDRIKEFMVLNPDIYVAGLREGCMFYVKDNYMHMKGIHPLRLFKFG
                     TETREIPAGEDLSFLLHR"
     gene            1010..2596
                     /locus_tag="PLK75_03440"
     CDS             1010..2596
                     /locus_tag="PLK75_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOY38329.1"
                     /translation="MNLLLMPYFSLFLIILIGYFLGKVKIAGISLDLAAVLFVAILAG
                     YWGFVIPSELKMIGLVMFIFSVGYQAGPSLIESFRKKGLRLILLGTVIVVSGLLVTLL
                     FAFIFGLKDTIAAGIFTGALTSTPGLAAIIEATHDANASIAYGIAYPFGVIGVVLFVK
                     LYPRIMRVRIQEAEKDYEKNLQGTHPGLYSKNFVVENPEIIGKSIAEIHFRKQTGANI
                     SRVLQNGAAFTPREETPLYKGSIIKAVGTLEALNKVEALIGKVTTVGIALSENYEVRF
                     ITVTNKQVLGKAISELEVHSKYNAVVTRIRRSGVELFPGYDVKLQFGDRIRVAGNKEN
                     LREVAGFFGDNENQVYESDFWPISLGIVLGVLLGLPKIHIGGFDLSLGATGGVLAMAI
                     IISRIGRIGPVVFSVSSHGNYLMRQFGLLIFMATIGTEAGADIIEVLSTKGWNLLLIG
                     FSVTIIPMIVGAIFGSLIFKMDILSFLGALTGGMTSTPGLGSVTSMTNTNAPGVAYAT
                     IYPIALVLMIVGVQIILAVL"
     gene            2642..2714
                     /locus_tag="PLK75_03445"
     tRNA            2642..2714
                     /locus_tag="PLK75_03445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Met,seq:cat)
     gene            2929..3330
                     /locus_tag="PLK75_03450"
     CDS             2929..3330
                     /locus_tag="PLK75_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOY38330.1"
                     /translation="MNHISFNLIGIFVNDLRAMVDFYHQVIGVEIEWDGEGPYAEFKH
                     KGIRFAMYERRMLPDLLGLQPSFPQGVNGSFELAVNVGDSHKVDKFFSKAVSAGAQPI
                     YFPRNEPWKMRSAMIADPEGNLIEIASDFWE"
     gene            3409..6849
                     /locus_tag="PLK75_03455"
     CDS             3409..6849
                     /locus_tag="PLK75_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOY38331.1"
                     /translation="MTNNTHNAEDINRLVQQTKERLKELACINQTTSIIKTGKSIDET
                     LQQIVDILPAAWQYPEYTCARIVFEKTTYRSKQFSESAWSMKQEFSTINNKNGIVEIY
                     YLKEFVNIDEGPFMFEERQLINNIAALIAGYLNSIAALKLIKNDDSPGLFSVPYKGGS
                     SNRKLLHKFLNENNYSRDLYHDLVPFNVKEILLVGTLYDAYSIEREGRFADHVLGEYR
                     QLNLAYTPRITGASTPEEVYEQISTKHYDLIIFFIGLEKRMPLEVSKIIKEKFSYIPI
                     YFLLNNNGDIAYYEENKPDSVDRIFVWNGDSNIFFAMIKSVEDKINVKNDTTMGLVRV
                     ILVVEDSAKFYSKLLPILYKVVLEQTRRIIDDVKADELYKVLRLRARPKILLANNYEE
                     AISIIEQYKDYIQCVITDLKYKKAGIVTPDAGYQLLEYVKNNLLDLPVCIQSSEVESS
                     FQRNHACINKNSDNYEEQIITFVNYFVGFGNFVFRDKDGQQLAVAKNLKAFQEYIEII
                     SDDSLIYHARRNHFSSWLMARGEISLAKLLNPYKLEDFKTVVDLRKFLIKQIHEFRDE
                     AQRGRLLPFDETVLTDERNVAHIGEGALGGKGRGIAFINTLIYDFDFDTLVENINIKT
                     PRTIVLCVDVFDDFIEENQLKDYYTQQIPFQELRKIFKDAQLPRYLVRQLKIILKKFD
                     KPLAIRSSSLLEDSLYQPFSGIFETYLIPNNHEDFEVRMEQFQDAIKMVYASLFSDMS
                     RGYIEAVNFKLEEEKMAIVIQEVVGNRFGNYYYPHISGVGQSYNYYPVSYMLPEDGFS
                     VMALGLGRYVVEGEKAFRYCPKYPKLDIASLKDQYKDSQVHFYAVDLKKKDIDILEGE
                     DAGLIKLDIYDAEMHGTLKHCASVYDIDNERILPGLDAQGPRIINFANILKYNYSPIP
                     ETVDKILEIVKEAMGTAVEIEYAVDLNKDEEGKTSLYLLQIKPLIGNVNDFEIDDSVI
                     NTDDIILRSVKAMGNGIIEDICDIIYIDLEKFDKSKTELMVEEIAVLNEKMKSEKKRY
                     ILIGPGRWGTRDKWIGVPVVWPQISNAKVIVELGFEGFPLDPSSGSHFFHNVTSMNVG
                     YLSIPSCNEEQFINWEKLSKNHVVERTEFFVHIETPLPFIVKMDGKKRISMIIQNNMV
                     "
     gene            complement(6858..7604)
                     /locus_tag="PLK75_03460"
     CDS             complement(6858..7604)
                     /locus_tag="PLK75_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOY38332.1"
                     /translation="MLHLFIYLIVTSLSGNFAGNYRDTCEKINPYNNVEFLTGKTFAP
                     SDSLFSALPPQCSNYGSQYLLCEAADSFNAMYAAAKKDGIVLQVVSAYRSFDIQKKLW
                     ENSWAKNRRSFRDDNGLSAYILRYLSMPCTSRHHWGTEVDVCSTALSWWQTLYGQKTY
                     RWLENNAAKYGFYQPYGPLGPVRKSGYQEEKWHWSFAPLASQFIVFYNNSVGYQHITG
                     FSGSNTAAFHRVIENYVLSVDSVPSGLIRL"
     gene            complement(7609..8547)
                     /locus_tag="PLK75_03465"
     CDS             complement(7609..8547)
                     /locus_tag="PLK75_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOY38333.1"
                     /translation="MNYRSKIATKLGFGQGLVHLGSTYGNVVCLGSDITASVGMNLFK
                     EAFPERFFSFGIAEQNIAAVAAGLALSEKIPFFSTYGVFASMRCADQIRVSICYNNVP
                     VKIGGAHAGISVGPDGATHQALEDISVMRALPGMLVLSPCDATQTRLATVASLEQWKG
                     PAYIRFGREAMPDFTDPTQDFRIGKGQVLREGTDLTLIGTGHLTWECLVAAELLANSG
                     IQARVVNIHTIKPIDSELIRDCAVKTGRIVTAEEHQIHGGLGSSVAEVVCQLYPVPMR
                     IVGMPDCFGESGEPLSLMKKYGLCAENIVMRAKEIL"
     gene            complement(8700..9149)
                     /locus_tag="PLK75_03470"
     CDS             complement(8700..9149)
                     /locus_tag="PLK75_03470"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOY38334.1"
                     /translation="MAKIRVTKKFEFEMAHALHDYDGLCRTVHGHSYKLDVTVIGTPF
                     TMYGHEKIGMVIDFSDFKKIVKDLIVDVYDHSLVISDKENLSSLSENSGLFNRIHIVP
                     YQPTCENMITHFAEILRNIFPGGIQLHSLKLHETANSFAEWFAEDQI"
     gene            9248..10222
                     /locus_tag="PLK75_03475"
     CDS             9248..10222
                     /locus_tag="PLK75_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOY38335.1"
                     /translation="MNTQGNITDIIQDDLKAFEKRLKEFTSSTNPYVSEMLDYILSSK
                     GKLIRPIMVYLSARMFGTPNETTHIAAVLLELIHSSTLVHDDVVDESSLRRGKPSVNA
                     IWENKRAVLIGDYMYANAMVIATQKGEHKLFDLISPTIMKMSLGELIQLDNSQKNNFE
                     ESVYLEVIENKTASLISTCCKAGAFSVGAGDEMQKRIGEFGVTCGMAFQIKDDILDYK
                     PANRTGKTTGNDILERKATLPLIGAIKNAENRITQNVLQYWKNGNDNNYLLSLVTKYV
                     LENKGIVYAENRMKEYYNTAAAQLNSFCDSPAKTAMQKVLEFVIDREK"
     gene            10250..11164
                     /locus_tag="PLK75_03480"
     CDS             10250..11164
                     /locus_tag="PLK75_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY38336.1"
                     /translation="MLNHYGEWAALIAAIFWTVTALAFENASKRIGSLAVNLLRLLIA
                     FVLFGVMAMIRTGSFFPVEASAHNWIWLSISGLIGFVIGDLMLFQAYVVVGARVAMLI
                     MSLAPPLAAVIGWAIMGETMQTTGLLGILITLAGIAMVILTRKNRDDDPKSEKKIKLK
                     YPVKGVLLAVGGAAGQATGLVLSKYGMQGYDVISSTQIRVITGAVGFVIIFAMMNKWR
                     ALKALKNDPGSIVSLGLGSVFGPFLGVSFSLIAVKYAAATGVAASIMSVVPVLIIAPA
                     VIFFKEKITLKEIAGAIVTTAGVVLFFI"
     gene            complement(11171..12025)
                     /gene="deoC"
                     /locus_tag="PLK75_03485"
     CDS             complement(11171..12025)
                     /gene="deoC"
                     /locus_tag="PLK75_03485"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOY38337.1"
                     /translation="MNTSGVFNESSQIGLMLTEIKSSVPKSIDSTVLKTILHCIDLTS
                     LNATDTNAGIKAFTEKVNLFSSHFNMDNVAAICIYPSLVPVVKQSLADKNVKIASVAG
                     SFPSSQTYLTIKLAECELAVNKGADELDIVISLGNFFGEQYQAISDEIALIKSLLPAT
                     TLKVILETGELKTLENIYKASWIAMQSGADFIKTSTGKSPVSATPEAVLTMCIAIRNY
                     FAETGRKVGIKASGGIVTPEDAFYYYNIVASQLGNDWLNNSYFRIGASRLANNILTSI
                     LSTPVTWF"
     gene            complement(12028..12351)
                     /locus_tag="PLK75_03490"
     CDS             complement(12028..12351)
                     /locus_tag="PLK75_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOY38338.1"
                     /translation="MTISESQQMVDQWIAQHGVRYFNVLTNMTILTEEIGELARVVAR
                     IYGEQSPKPNEKLNLSEEMADVLWVLICLANQTGVNLEEAFLQTITKKTNRDSLRHIN
                     NPKLK"
     gene            complement(12362..12727)
                     /locus_tag="PLK75_03495"
     CDS             complement(12362..12727)
                     /locus_tag="PLK75_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38339.1"
                     /translation="MTILFFLTMSCTSDNVDGGAKGIINFGEGDCSLDYTFRFYSKYT
                     GYAYCIEQSVNDSLQNIYNVYPYCDSVFCSNGKYNFALEPGIYYIFIREYPFRSDENK
                     ITVHYKSIAETEFFFYRCI"
     gene            complement(12764..13219)
                     /gene="dtd"
                     /locus_tag="PLK75_03500"
     CDS             complement(12764..13219)
                     /gene="dtd"
                     /locus_tag="PLK75_03500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOY38340.1"
                     /translation="MRVIIQRVSTANVKILNKTVGQIATGLVIFLGIVSDDTKEDVDW
                     LVNKIINLRIFADDSGVMNRSLLDISGDILLVSQFTLHAKTKKGNRPSYYLAATPNIA
                     IPLYELFIHKCNSLLPKPVQTGIFGANMNVFLCNDGPVTIIIDSKNKDL"
     gene            complement(13263..14897)
                     /gene="ade"
                     /locus_tag="PLK75_03505"
     CDS             complement(13263..14897)
                     /gene="ade"
                     /locus_tag="PLK75_03505"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOY38341.1"
                     /translation="MKIEGNLIDIFKKEIYPAAITINNNGIITSIDRIDKQLDIFISP
                     GLIDAHVHIESSMLIPSEFAKLAVKCGTVATVSDPHEIANVIGKNGVEFMINNGKQTP
                     LKFHFCAPSCVPATGFETSGASLSAQDIDSLFEKQPIVALGEMMNFPGVIYNMPEVIE
                     KLNIARKWQKPIDGHIPGISGDELRKYISEGISTDHECFSLDEAIAKINLGMKIIIRE
                     GSSAKNFDALCPLIDTHPDMVMLCTDDSHPDDLINKGHIDKIIRMGLNKGLSIFNLLA
                     ASSLNTIQHYGLNVGCLRVGDNADFIVMDNIDTFHVTETYINGKPVYSSGRILFNTPK
                     AVAVNNFAINTPISSNDIVVNAVVESSIRIIDATDGSLLTGTLIEKPLIANNKVVSDI
                     SRDILKIVVLNRYSEISPSVGFIHGFSLKQGAIASTVAHDSHNIISIGVSDSDICDAI
                     NELIAHKGGITCNDGNKNFYTIPLPFAGLMSDESGDKLALQYGIMNHAVKELGCNLTS
                     PYMTLSFMALLVIPSLKIGDKGLFDISAYSFQDLFV"
     gene            complement(15000..16874)
                     /gene="mnmG"
                     /locus_tag="PLK75_03510"
     CDS             complement(15000..16874)
                     /gene="mnmG"
                     /locus_tag="PLK75_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOY38342.1"
                     /translation="MLTHYDVIVVGGGHAGCEAAAAAANLGSRTLLISMDLTKLAQMS
                     CNPAMGGVAKGQIVREIDALGGFAGIVTDNTMIQFRMLNMSKGPAMWSPRAQCDRMQY
                     SLEWRNTLENMPFLDFWQDSVTSLQIEGSTITGVVTQMGIHFHSKSVILTTGTFLNGL
                     MHIGQTQIQGGRIGESASYGITEQLLSFGFRGDRMKTGTPVRIDGRTVDFSQLQEQKG
                     DEMIVPFSFMHPYGYHKLKQRSCYICNTDAEVHDQLKTGLDRSPLYDGTIQSIGPRYC
                     PSIETKLVTFPDKDSHQLFLEPEGENSIEFYLNGFSSSLPWDVQLSSLQKIKGFEKVK
                     IFRPGYAIEYDFFDPTQLNANLETKIINNLFFAGQINGTTGYEEAGAQGIIAGINAHL
                     KIKNKPEFTLSRDQAYIGVLIDDLVTKGVDEPYRMFTSRAEFRILLRQDNADFRLTEI
                     SHNLGLASDARMKRFEKKSQQISSIENFSRKYSCNPVEINPWLESADTSPLQQKIKLN
                     TLLTRPQVSLEALLSQIPSLQALISDFDNKTESLLTAEIMIKYSGYIEREKLMAEKLK
                     RLETIELSGFIDYDNISSLSTEGRQKLKRIKPKTLGQASRISGVSQSDLSVLLIYLGR
                     "
     gene            complement(17015..18946)
                     /locus_tag="PLK75_03515"
     CDS             complement(17015..18946)
                     /locus_tag="PLK75_03515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38343.1"
                     /translation="MKVIRFYHIFFTIIWAFFLGFTHISYAQKATNEFQSDTLSYYYI
                     RLAMDNVDSSGSQAEVYARKAIEISKSHNDLKSLGDAYNALALWYQANSQLSKALAYY
                     DTVVFYASAANDNFRLNTAYNNIGVIYRQQQSLEPAIQYFEKSLYYAYACRDTLSIVY
                     CLSNIGNAYAVLGQYEKALSRYIKAYNLLKELPETTEELAYINNNIAGIYRRLKKFDF
                     AVSQYNLAYAGFSNCENINAMLSVSIALAQTYLDWAKPAESFQSIEMGANLCDKASDK
                     ALKESFYNQAYKTCLANNNISEALKYLELYASARETNYDAELKQMLVETGQKYELDKI
                     TRKKKEQERSLRQQRIIIISLALVVLLSLLIALLFAMIFIQKNRLNKKLKQKNTIIES
                     QNLQITESISYAKQILALQNVEDVFPKEYCSKAFVWYQPKEVVGGDFYRIAEKDDCLT
                     IAVGDCTGHGAAGGFLAASYLRLFDRAMHEASGGPATLFTHLNRLQSEFFGSERDVNI
                     KHLRSCAMSIIMIEKNNGVIHLVSASQKVIVVSEEGTRIFSGDKLMLGNDDFFVATEH
                     RFSLQAGDKIYLFTDGITDQLNRNKTRKITANGLSGILTQENHKDLNDIELLLKSHWL
                     LWKGEASQTDDILVLGIAF"
     gene            19077..20135
                     /locus_tag="PLK75_03520"
     CDS             19077..20135
                     /locus_tag="PLK75_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38344.1"
                     /translation="MFVRVPEKQTDVGVFQVVLRNHNFFKNNEYFNDYVYSYTLLGAF
                     VAPELVYSISNKLIISGGFYAQKYSGKNDLNKIKPLYRVVYCPISEFSITMGNIKGGA
                     EHHLTEEIYCADRHIIDNNEEGLQFMYSKGSWFADLWLNWDQTAFPNDRKQEQLQLGF
                     SGEFPLLSKKKTVFTGVGQFLWAHNGGQDLAVTYDVRTIGSVAGGLRYRQNFYSKIPA
                     GIGAECMYVACRDLSPFLKLPYSQGYGVHSKVSAYFSYFSLDLAHWYGYHYFSAFGEP
                     LFQSVSQKWGSALAPERNVFTIKAAFTKEVADGLKIVVHYGGFWGKDIERFDYFYGMH
                     LIINQNILINKVNRLKSL"
     gene            20132..20587
                     /locus_tag="PLK75_03525"
     CDS             20132..20587
                     /locus_tag="PLK75_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38345.1"
                     /translation="MRGITSGILILIAFTIGSCSSAEKEVESTYPDGKPLKVVYYKDI
                     EGVRDLVKTEYYYTNGWLESETNYKNGKKNGKCLMYFTNGKVASQEYYCDDELHGKCH
                     YFSETGKRRFTASYDHGISDGKWIIYDENGKKATVQIFENGKLIKQRNK"
     gene            20584..21138
                     /locus_tag="PLK75_03530"
     CDS             20584..21138
                     /locus_tag="PLK75_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38346.1"
                     /translation="MKTRINVFIPVLLFFSLLCGCNGNNVSSQSRIAEREKNERTIKY
                     AKPASDTIAKNYVCQVVASFQNEQPQKVFYFRDSSFQELVYEIQYYENGHRKMEGGYS
                     NGKRSGKWIAWYENDTVWSYGYYRNGLRHGVSAVYFPNGQKYYDKSYFNDTAEGKWTF
                     YNEQGKLVGEAFYHNGKLMRETKY"
     gene            complement(21150..23378)
                     /gene="purL"
                     /locus_tag="PLK75_03535"
     CDS             complement(21150..23378)
                     /gene="purL"
                     /locus_tag="PLK75_03535"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOY38347.1"
                     /translation="MTEKPVTLATAQELGLNEFEFDLIQKYLNRIPNYTELSIYSVMW
                     SEHASYKNSIKWLKTLPRDGGCLLTGAGEENAGLVDIGNNLACAFKIESHNHPCAVEP
                     YQGAATGVGGINRDIFTMGARPIAQLNSLRFGNIKNDRTKWLMKGVVKGIGDYGNSFG
                     VPVLGGEVFFDDSFNTNPLVNAMSVGIMEKGKVISAIAKGAGNPVFIVGSSTGKDGIH
                     GATFASADITEDSADDIPSIQVGDPFQEKLLLEASLELRDSGAIVGMQDMGAAGIICS
                     TSEMSAKGNSGMRIDLSLVPLRQKNMEAWEILLSESQERMLVVVEKGKEKIVNDIFTK
                     WDLNCQQIGEVIDEDRLYFHYQGKLVAEVPANSLVLGGGAPVYERSYQAPEYVQNHKI
                     FNIDTIPEPEDLKDIADFLTQHPNIASKRWVYEQYDTMVRTNNMSTNNPSDAGIINIK
                     GTNTALAVSVDCNSRYVKADPETGAMIAVAESVRNVACSGARPLAITNCLNFGSPYNP
                     EAYWQFVMAVKGMGTACRKFNTPVTGGNVSFYNQTTIAGKTEPVFPTPTIGMLGILDD
                     KRSQTGLAFTRKGDVIFLIGNVYNDINSSEYLYSYHKVKNSPPPHFNLDTEHKLLQVI
                     MALTGTNIVASLHDVSDGGLYIALLESAMPNKLGFDITTAAEVRKDAFLFGEAQGRVV
                     ASVPPENQNRFIDVLMSFEFPFTVLGHVTKGEIRIDDLPFGFIKDARKLYNRSIESEM
                     LK"
     gene            23519..24871
                     /locus_tag="PLK75_03540"
     CDS             23519..24871
                     /locus_tag="PLK75_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38348.1"
                     /translation="MLQEILLSLALIFFFVFLIMKLKFFRRLGIKTRVLVLVFLLKLV
                     TGVGVSFIYTHYYDPWTADMYKYFHGGRALYSAMEESPSDYFKMLTGIHANEPQLTKY
                     YDYADYWYKKYNYGLYNDNRTMIRYNAFLCLISQGNYYVNLVITIFLSFCGAFLLALG
                     LHKCMPGKNLLLFFCIFLVPTVLFWSSGLLKESLVMFSMGAMVYFLIRFKELLKVKYL
                     LFFIVTAVLLFFSKFYVLLALTPGMVFYMVVKKNNYWRQTLVMSIIFGVILILFFSSK
                     SITGLDFAKIISEKQTDFVKFVNLAEDAGSNVDLPQVDPSPQGIISHLPVAYYHSFFR
                     PNIFEGGSVFSRMAAFENTGVIFLCILLLIYFRKPDKQEYKVILFSGSFILILYALIG
                     LTTPNLGALVRYKMPAMPFLILIICLCINTKKMPLIINKGIQILSDMVIEKPLTHQEH
                     "
     gene            complement(24852..25598)
                     /locus_tag="PLK75_03545"
     CDS             complement(24852..25598)
                     /locus_tag="PLK75_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOY38349.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKIFSRIAKEITIAIKSGGSADP
                     EFNARLRVAIQNAKGANMPKDNIERAINKGGDSSALFESTFEGYAPHGIAVFVECMTD
                     NNNRVVSNVRSIFNKYNGSLGTNGSLSFLFERKGVFTILNNGKIDSDELELELIDGGA
                     QDIEIDEETITVYCALEDFGNLNKKLEQLKVDIESAELQRIPNDTKTLDVDTAKKVMK
                     LIDALEDDDDVQNVYHNLEITEELANALDE"
     gene            complement(25687..27723)
                     /locus_tag="PLK75_03550"
     CDS             complement(25687..27723)
                     /locus_tag="PLK75_03550"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOY38350.1"
                     /translation="MKTFRQYILLDIMLFVWLVLPSGVYSQSYQTQVSSPNAINEICF
                     ILDTAGNAFYTVQHKGDTLLGASRLGIVLQNDSLNTFDFITKSEKQVSENWSPVWGIS
                     SSVQNNYNSITVNLRKKNGKSRVNVEFRCFDDGIAFRYILTGGSKGSVVVIMSEDTRF
                     NLYGTWESWWSYADYNTLEKEYYHTAADSAQHVATPFTLKNQNGKYICIHEAAITEYT
                     TMTLRQDSNLNNAYQVNLVPWADGTAVKTRAPVNSPWRVVFIADTAGDLLVSNLLYNL
                     NDECIINDVSWIQPMVYNGIWWEMHLGISTWDKGPKHGATTVNALRYIDFSAENQWGG
                     CLIEGWNTGWENWGKPEAFDFVTPYSDFDLRKVAEYAAAKNVVLIGHHETGGDIVSYE
                     YALDSAFSLYQSMGIRAVKTGYAGAIVPAGEYHHGQYMVEHCNMVMRKAAQYHLMLDV
                     HEPVIMSGLSRTYPNLMTSEGVRGMEWNAWSKGNMPRHTCILPFTRGMAGPVDYTAGI
                     FDIDLSAHRDKRVKWNSLDPGNTAVHSTIANQLALMLVLYSPMQMAADLPENYKDHPA
                     LDLLRQLPTTFDETRVPDAEIGEYIIITRRKGEIWYLAAITNEKSREFEIALNFLNEN
                     THYQVDIYEDGETADYRYNPEAITVKKQFVGKTDVLKFRLASGGGYIAVFRIID"
     gene            27978..28232
                     /gene="rpsT"
                     /locus_tag="PLK75_03555"
     CDS             27978..28232
                     /gene="rpsT"
                     /locus_tag="PLK75_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOY38351.1"
                     /translation="MAHHQSAKKRIRQTEARRVQNKYAAKTTRNAVRKLRSVTDKKEA
                     EKLFPEVASMLDKLAKNNVIHHKKAANLKSSLAVQLNKMQ"
     gene            28367..29053
                     /gene="radC"
                     /locus_tag="PLK75_03560"
     CDS             28367..29053
                     /gene="radC"
                     /locus_tag="PLK75_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOY38352.1"
                     /translation="MKSKSIKNWAVDDRPREKMINKGTVSLSDAELIAILLRTGNRDK
                     SAVELAREILDANSNNLNLLGKQDISHFKKFKGIGQAKAVEIIAALELGRRRKLAEIP
                     EYKSITSSKDAFSMFEPMLADLSHEEFWVALLNRANHVTDIFKISQGGTAGTVMDVKI
                     IMKRALEKLAHGILISHNHPSGNNKPSDADRQITHKLRDAAKLFDITLLDHIIVANKQ
                     YYSFADEGML"
     gene            29080..30372
                     /locus_tag="PLK75_03565"
     CDS             29080..30372
                     /locus_tag="PLK75_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY38353.1"
                     /translation="MIQVLVNNETPRVKYALSLVFRQVLHTEFEIITNLDQVNGFCIN
                     YSGNPAEGLLKIWPHPILNEDEIPAYNPVVSSWNELPVFFCSPDGFDIPFDVFAASFW
                     VTTRYEEYSTSETDFMGRYKASASHAARNGYLKKPVVNLWAAELKKLILQKDSSVVFG
                     EMDYRFVPTIDVDSMYKYKYKGFVRNTGGFFRDLFTGRFSNCSERLNVLLNRKRDPWD
                     CLGEIEKIHIENGMLPMYFILAAKRSKYDKNIPANSSQFAKNVLALQHNCRLGIHPSF
                     AGHCDENAWKNEKKTLEGILRTPVYEARVHFLKLTFPDTYQKLLELGIQFDFTMGYPE
                     TTGFRAGICSPFFFFDLTENRETELCVVPLGMMDVTLSKYMKMNTEEAKIAVAEIVKE
                     IRQVEGVFVSLWHNESFGEDVDNAHTENLYRYLLKVAK"
     gene            30373..31287
                     /locus_tag="PLK75_03570"
     CDS             30373..31287
                     /locus_tag="PLK75_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38354.1"
                     /translation="MKINYLKHNAIDFNAWDECISSSVNPRIYATSSYLNIVSPGWDA
                     LITDDYRCVMPLTHKKKFGVSYIFHPPLTQQLGIFSIEFTNTETTKKCIEAIPRKFRL
                     VDMPMNTAMPMDQSLYSEQGITHHLSLQPGYTELHSGYSENTCRNIARAVKNQLHVTQ
                     SSDFGVISYMINAGCQPWVGGLGKETIKDVEKKLLQLLINGIGSLFTVLDSNNEVFAA
                     AVFSQYRGYITYLFGISSDTGKDKRAMFLAFDTIIHTNSGSNIILDFEGSSIEGIARF
                     FRGFGATPVYFPKLKINNLPAAMRYFKK"
     gene            31327..31980
                     /gene="upp"
                     /locus_tag="PLK75_03575"
     CDS             31327..31980
                     /gene="upp"
                     /locus_tag="PLK75_03575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOY38355.1"
                     /translation="MKIVNLGGKGTLLDQYLAEIRDEAIQCERMRFRKNMQRAASIFA
                     YEVSKALPFTDEEIQTPLGVAIVPTMKEQPVIASVLRAGIPMHEGVLDFFDHADSAFI
                     AGYRKYENNDTFDIQIDYITSPSIEKRLLIICDPMLATGQSMVLAYRGLLKHGKPRHT
                     HIISLIASKEGVEHLQKYLPDNEVTIWVGAIDDELTVKSYIVPGLGDAGDLAFGLKE"
     gene            32051..>32493
                     /locus_tag="PLK75_03580"
     CDS             32051..>32493
                     /locus_tag="PLK75_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38356.1"
                     /translation="MKKFVIGLLFLISIVTFWSCENAINITQPTILVESAQLSVFDSV
                     SISATENADIDGNRVVPNYYEWKILDYSENTLYTDFPDENNITWVPDSAGNFIISVKI
                     GYDGNKSVTTLREVTVTECPASMQQRLIGHWTGTAEAMFGATWNI"
BASE COUNT         9758 a         6360 c         6674 g         9701 t
ORIGIN      
        1 aagttataga tttgtgtcta taattttgtc tgatttttta aggggctaaa acactgaaca
       61 aaatttgttc tgacaaatac ttcaaattat aagtaaaaag aatcattaaa acattaggga
      121 ataaatgcta taattgtatt tagtttctaa tattaacact ttaacttatt aaataaacat
      181 catgcaacta ttacttatca gcaattcgac aatggccggc gaagcctatc tcgattaccc
      241 aaaatacgat atccgcgatt ttcttggaaa taaacctgta aaagccttgt tcattcctta
      301 tgcgggagtc acagtaagct gggacgatta cgaagccaaa gtaaagaatc ggtttaacga
      361 agtgggccat gatgtgattt caatacatcg tttttccgat ccggtaaaag ccgtcgaaga
      421 agctgaagct attgtggtgg gaggtggaaa cacatggaat ttactgcgta aaatccatga
      481 taataagctc ttagaaccca ttcgcagcaa agtattgtcg ggcacgaaat ttattggctg
      541 gagtgcgggg tccaatgtag catgccccac tattatgact accaacgata tgcccataat
      601 cgacccgatt ggtttcgatg cttgtaatct agtgcctttt cagattaacc cgcattacct
      661 cgatgcgaat ccggaaggcc acggaggcga gacccgcgaa gaccgtatta aggaatttat
      721 ggttctgaac cctgatattt atgtagccgg attgcgcgaa ggatgcatgt tttatgtgaa
      781 agacaattac atgcatatga agggcattca tccgttaagg ttatttaaat ttgggacaga
      841 aacccgcgaa attcctgccg gcgaggactt gagttttctg ttacatcgtt aacaaaactc
      901 tgttctaaac cgaactcgac aactatttaa aaacataggt ttatagtatt gttaagcatt
      961 ttttatataa atgccctcag tgtactttta gttgtctcta ataacccgaa tgaatctgct
     1021 attaatgcca tatttttcac ttttcctgat aattctgata ggatattttt tagggaaagt
     1081 taaaatagca ggaatttctc ttgatctggc ggccgttctt tttgttgcaa tacttgcggg
     1141 ttattgggga tttgtaattc cctctgaact taaaatgata ggcctcgtaa tgttcatttt
     1201 ttcggttgga tatcaggccg ggccgtcgct gatagaatca ttcagaaaaa aggggctgag
     1261 acttattctt ctgggaaccg tcattgttgt ttccggtttg cttgttaccc tgctttttgc
     1321 ctttattttt gggttaaaag acactattgc ggctggaata tttaccggag ctctgaccag
     1381 tactcctggg cttgcagcta taattgaagc aacgcacgat gccaatgcgt cgatagccta
     1441 tggtattgct tatccgtttg gagttatcgg agtagtatta tttgtaaagc tttaccctcg
     1501 aattatgaga gtccggatac aagaagcaga aaaagactac gaaaaaaacc tgcaaggcac
     1561 tcacccgggc ttatattcta agaattttgt tgttgaaaat cctgaaatta ttggtaaaag
     1621 catagctgaa atacattttc gtaaacagac aggggcaaat atttcaaggg ttttacagaa
     1681 tggagctgca tttactccgc gcgaagaaac gccattgtat aaagggtcca taattaaagc
     1741 agtaggaacg cttgaagctc tgaataaagt agaagcactt ataggtaaag ttaccaccgt
     1801 tggtattgct ttaagcgaga attacgaagt acgttttatt acggttacca acaaacaggt
     1861 attgggtaaa gccattagcg aactggaagt acatagcaaa tataatgctg tcgttaccag
     1921 aatcagacgt agcggagttg agcttttccc gggttacgat gtgaagctgc agtttggcga
     1981 tagaatacgg gtagcgggga ataaagaaaa cctgcgtgaa gttgccggtt ttttcggtga
     2041 taatgagaac caggtttacg aatctgattt ctggcccatt tcgctcggta ttgtgcttgg
     2101 agtgctgctg ggcttaccaa aaattcacat tggtggattc gacctgtcgt tgggtgctac
     2161 cggaggtgtg ctggcaatgg caattattat cagccgtatt gggcgcattg gccctgttgt
     2221 tttttcggtt tcgtcgcacg gtaattacct gatgaggcaa ttcggtttat taatatttat
     2281 ggccacaatc ggaacagaag ccggtgccga tattattgaa gttctttcta caaaaggttg
     2341 gaatttgcta ctaataggct tttcagttac aattatacct atgattgtag gtgcaatttt
     2401 cggatctctt atttttaaaa tggatattct ttcgtttctg ggtgcactta ccggcggtat
     2461 gaccagcact cccgggctgg gttcggtgac ttcgatgacc aataccaatg cacccggagt
     2521 tgcttacgca accatctacc ccattgcact tgtgcttatg attgttggtg ttcaaattat
     2581 tttggcagtt ttatagtaat ttgtaaataa ttgaaaatat ttactatctt tgcacccaca
     2641 ttgcggggta gagcagttgg tagctcgtcg ggctcataac ccggaggtcg caggttcgag
     2701 tcctgtcccc gctacaaaaa aaagtgagtg tgagagtgag tacagagtac aaaacatcac
     2761 actcactttt ttttcactta cacaccaaaa tagagatggt ttatttttat agtttccctt
     2821 tgcaagagag tacatagctc attttgtctc gctcactgag ttttagcgat taattcctga
     2881 ttaaggaata tcggtttgat tttgtatttt taccaaaaac agttctgtat gaaccacatc
     2941 agctttaacc taattggaat atttgtaaac gatttacgcg caatggttga tttctatcat
     3001 caggtaatcg gtgtcgaaat tgaatgggat ggagaaggcc cttatgctga atttaaacat
     3061 aaagggattc gatttgccat gtatgagcga cgcatgcttc ctgatttgct gggattacaa
     3121 ccatcgttcc cacagggagt aaatggtagt tttgaacttg ccgttaatgt gggcgattcc
     3181 cacaaagttg acaagttttt cagtaaagcc gtttctgcgg gtgcccagcc aatatatttt
     3241 ccccgcaatg aaccatggaa aatgcgttca gccatgattg ctgaccctga aggaaacctt
     3301 atcgaaattg cctctgattt ttgggaataa acgcggataa aatttgccct ataacaactt
     3361 ttataagttg ttatttaatt atattgcgtt taattaagcc ccattgtcat gacaaataat
     3421 acccataatg ccgaagatat caaccggttg gttcagcaaa ccaaagaacg ccttaaggaa
     3481 ctggcctgta taaaccaaac aacatcaata ataaaaacag gtaaatcgat tgatgaaacc
     3541 ctacaacaaa ttgtcgacat tttacctgca gcttggcaat accctgaata tacatgtgcc
     3601 cgcattgtat tcgaaaaaac aacgtaccgg tcaaaacaat tttctgaatc agcatggtcg
     3661 atgaaacagg aattttctac tattaataat aaaaacggga ttgttgaaat ttattacctc
     3721 aaggagtttg tcaatatcga tgaagggcca tttatgtttg aagaacggca gttgataaac
     3781 aacattgcgg cattgatagc cggttacctc aacagtatcg ccgcattgaa actgataaaa
     3841 aatgatgaca gtcccggact tttcagtgta ccctacaaag gaggttcttc aaatcggaag
     3901 cttttacata aatttttaaa cgaaaacaat tattccagag acctctacca cgatctggtg
     3961 ccttttaatg ttaaagaaat attgcttgta ggtaccttat acgatgcata tagcattgaa
     4021 cgcgaaggtc gctttgccga ccatgttctg ggagaataca ggcagttaaa tctggcctat
     4081 actccgcgaa taaccggagc ctctactccc gaagaagtat atgagcaaat aagcacaaag
     4141 cattacgacc tgattatttt ctttattggg cttgaaaaac gtatgcctct ggaagtaagt
     4201 aaaataataa aggagaaatt ttcgtacata cccatttatt ttctgttgaa caacaatggt
     4261 gatattgcct attacgaaga aaataaacct gacagtgttg atcgtatttt tgtttggaat
     4321 ggcgattcca acatattttt tgccatgata aagtcggttg aagacaaaat caatgtcaaa
     4381 aacgatacaa caatgggctt ggtacgtgtt attcttgtgg tagaggattc tgctaaattt
     4441 tattccaagt tattacccat tctatataaa gttgtacttg agcagacaag acgaataatt
     4501 gatgatgtga aagccgatga attgtacaaa gtattgcgat tacgcgcccg gccgaaaata
     4561 ttgcttgcca ataattatga agaagccatt tcgattattg agcaatataa agattacatt
     4621 cagtgtgtaa ttaccgactt gaaatataaa aaagcgggta ttgttacacc ggacgccgga
     4681 tatcagctat tggaatatgt taaaaataat ctgctcgacc tgccggtgtg tattcaatcg
     4741 tcggaagttg aaagctcttt tcagcgtaat catgcttgca tcaataaaaa ctccgacaac
     4801 tacgaggagc agattattac gtttgtaaat tactttgttg gtttcggtaa ctttgtattc
     4861 cgcgataaag acggacaaca gcttgcggtt gctaaaaacc tgaaagcatt tcaggaatat
     4921 atcgaaataa tttctgacga ttctctcatt tatcatgcgc gcagaaatca tttttcgagc
     4981 tggcttatgg ccagaggaga aatcagcctt gcaaaactgc ttaacccgta taaattggaa
     5041 gactttaaaa cagtggttga tttaagaaaa tttctaataa aacaaataca cgaatttcgc
     5101 gatgaggctc aacgtggacg cttattgcct ttcgacgaaa cggtgctcac cgatgaacgg
     5161 aatgtagctc atatcggcga aggagctctt ggaggaaaag gtcgggggat tgcctttatc
     5221 aataccctga tttacgattt cgacttcgac acgctggtcg aaaatattaa tattaaaact
     5281 ccgcgcacca tagttctctg cgtcgatgtt ttcgatgatt ttattgaaga aaatcaactg
     5341 aaggattatt acactcaaca aataccgttt caggaacttc ggaaaatatt caaagacgcc
     5401 cagttgccac ggtatctggt acgccagtta aaaattattc ttaaaaaatt cgataagcct
     5461 cttgcgataa ggtcgtcaag tttgctagaa gattctttat atcagccatt tagcggaatt
     5521 ttcgaaacct accttatacc caataaccac gaagattttg aggtaaggat ggagcaattt
     5581 caggatgcaa taaaaatggt ttatgcatca cttttctccg atatgtcgag gggatatatc
     5641 gaagcggtta actttaaact ggaagaagaa aaaatggcaa tcgttatcca ggaagtagtg
     5701 ggcaacaggt ttggaaatta ttattatccg catataagtg gtgtagggca gtcatataac
     5761 tattacccgg tatcgtatat gcttccggaa gacggttttt cggtaatggc tttgggcctt
     5821 gggcgatatg tagtagaagg agaaaaagca ttcagatatt gccccaaata ccccaaattg
     5881 gatattgcca gcctgaagga tcagtataaa gattctcagg ttcatttcta tgctgtagat
     5941 ttaaagaaaa aagatattga cattcttgaa ggcgaagatg ccggattaat taaacttgat
     6001 atttacgatg ctgaaatgca cggtacactg aagcattgcg catcggtgta tgatatcgac
     6061 aacgaacgca ttcttccggg tttagatgca cagggaccca gaattattaa ttttgccaat
     6121 attctgaaat acaactactc tcctattccc gaaaccgtgg ataaaattct cgaaatagtt
     6181 aaagaggcca tgggaactgc ggtagaaatt gaatatgctg ttgatttgaa caaagacgaa
     6241 gagggcaaaa cttcgcttta tttacttcag ataaagcctt tgataggcaa tgtcaatgat
     6301 tttgaaatcg acgacagtgt cattaatacg gatgatatta ttctgaggtc ggttaaagcc
     6361 atgggtaatg gaataattga agatatctgc gacattattt atatagatct cgaaaaattc
     6421 gataaatcga aaactgaatt gatggtcgaa gaaattgcgg ttttaaacga aaaaatgaaa
     6481 agtgagaaaa aacgatatat tcttattggc cctggtagat ggggaactcg cgataaatgg
     6541 attggtgtac cggtggtttg gcctcagatc agcaatgcca aagtaattgt tgagcttggt
     6601 tttgaaggtt ttccgttgga tccttcatca ggatcacatt ttttccataa tgttacatcg
     6661 atgaatgtgg gatatctgag cattccaagt tgtaatgaag agcaattcat taactgggaa
     6721 aaactatcga aaaatcatgt ggtggagcga acagagtttt ttgtgcatat cgaaacccca
     6781 ttaccgttta ttgttaaaat ggatggtaaa aaaagaattt ctatgattat tcaaaataat
     6841 atggtgtaat tacgctgcta cagacgaatt aaacccgatg gaacagaatc aaccgataaa
     6901 acatagttct cgattacccg atgaaaagct gcagtatttg agcctgaaaa acctgttata
     6961 tgctgatatc ctaccgaatt attataaaat actatgaact gcgaagccag cggggcaaat
     7021 gaccaatgcc atttttcttc ctgataaccg gatttccgga ccggacccag cggcccatag
     7081 ggttgataga acccatattt tgcagcatta ttctccaacc atcgataggt cttttgtcca
     7141 tatagtgttt gccaccacga tagcgcagta ctgcagacat cgacctctgt tccccagtga
     7201 tgccttgaag tacacggcat agacaaataa cggagaatat aagccgacag cccattgtca
     7261 tctctaaacg accggcggtt ttttgcccat gaattttccc aaagtttttt ctgaatatcg
     7321 aagcttcggt aagctgatac tacctgaagg acaattccat ctttttttgc tgcagcgtac
     7381 attgcgttga aagaatcggc agcctcacat agtaggtatt gagatccgta attcgagcat
     7441 tggggaggca aagctgaaaa caaactatcg gaaggtgcaa atgtttttcc tgtcaggaat
     7501 tctacattat tgtacgggtt aattttctca caggtatctc ggtaattgcc ggcgaaattg
     7561 cccgacaagc ttgttacaat aagatatata aacagatgta acattatttt acagtatctc
     7621 ttttgctctc attacaatgt tttcagcaca aagtccatac tttttcatta gtgataacgg
     7681 ttctcccgat tcgccaaaac aatcaggcat cccaacaata cgcataggaa ctggatacag
     7741 ctgacataca acttcagcca ctgaacttcc caatcctcca tgaatttgat gctcttcggc
     7801 tgtaacaata cgaccggttt taactgcaca atctctgatc aattccgagt cgataggttt
     7861 aatagtatgt atgttcacta ctctagcttg aattcctgaa tttgcaagca attcggcagc
     7921 aacaaggcac tcccatgtaa gatgcccggt tccaatgagt gttaggtcgg tgccttcgcg
     7981 aagtacttgc ccttttccga tgcgaaaatc ctgagtcgga tcggtgaaat ccggcatggc
     8041 ttcccggccg aagcgtatat acgccgggcc tttccattgt tctaaggaag ccacggttgc
     8101 caggcgggtt tgggtagcat cgcagggcga aagtacaagc atccccggaa gcgcccgcat
     8161 aaccgaaata tcttcaagag cttgatgggt agcaccatcg ggaccaactg atatacctgc
     8221 atgagcccct ccaattttga caggtacatt attatagcag atcgaaaccc gaatctgatc
     8281 ggcacaacgc atggaggcaa atactccgta ggtactgaaa aacggaattt tttcgcttaa
     8341 ggccaaacct gccgctactg ccgctatgtt ttgttcggca ataccaaacg aaaaaaaacg
     8401 ctcagggaaa gcttccttaa ataagttcat tcctacagaa gcagtaatat cagaaccaag
     8461 gcaaaccaca ttgccataag tagatcccaa atgtaccaaa ccttggccaa aacccagttt
     8521 tgtagctatt ttgcttcggt agttcattgt ttttggcgta aagatatgaa aaatacccgc
     8581 agtattaccc ttgtaatttt gtctgtatta ccgcaaaagc aataaacaat aatgataatt
     8641 ttaaacacgg aagtaccaga aatatttaaa agctcatgaa aataaaaaat aatgaaattt
     8701 taaatctggt cttcagcaaa ccattcagca aacgaattgg ctgtttcatg aagcttcagt
     8761 gaatgtagct gtattcctcc agggaaaata ttgcgtagga tttcagcaaa atgagttatc
     8821 atattttcac aggtaggctg ataaggaact atgtgaatcc ggttaaataa cccggagttt
     8881 tccgataacg aggaaagatt ttccttatcg cttattacaa gagaatggtc atatacatca
     8941 acaataaggt ctttgacaat ttttttaaag tcggaaaaat caattaccat tccaatcttt
     9001 tcatgaccat acattgtgaa aggagtgcca atcacggtaa catccagttt gtaggaatgc
     9061 ccatgaacgg ttctgcacaa gccgtcgtaa tcgtgcaacg cgtgagccat ttcaaactca
     9121 aacttcttgg taactcttat ctttgccata taattttaca aaataaataa acaaaagaaa
     9181 acaaatattt gttcaaacat attaaatatt gaaacatata cagtaattta gttgtttaat
     9241 ttgaaaaatg aatactcagg gaaacattac agatattatc caggatgatc ttaaagcatt
     9301 tgaaaaacgc ctaaaagaat ttacatccag cacaaacccg tatgtttctg aaatgctgga
     9361 ctacatatta agctcaaagg gaaagcttat aagacccatt atggtgtatc tatcggcccg
     9421 aatgtttggt acaccaaacg aaacaaccca cattgcggct gtcttgctgg aactaataca
     9481 ttcttcaacc ctggtacatg atgatgttgt agatgaatca tcactccgaa ggggaaaacc
     9541 ttcggtgaat gcaatatggg aaaacaaacg ggctgtttta attggcgatt atatgtatgc
     9601 aaatgctatg gtaatagcaa cgcaaaaagg agagcataaa ctgttcgacc tcatttcacc
     9661 gacaataatg aaaatgagtc tgggagaact tatacaactc gacaactcac aaaaaaacaa
     9721 ttttgaagaa tcagtttatc ttgaagttat tgaaaacaaa accgcctcac tgattagtac
     9781 atgttgtaaa gcgggagcat tttcggttgg tgcaggtgat gaaatgcaaa aaagaatcgg
     9841 agaatttgga gtaacatgtg gtatggcctt tcagataaaa gacgatattc tggattataa
     9901 acctgcgaac cgaaccggaa aaactaccgg aaacgatata cttgaacgaa aagctacatt
     9961 gccattaata ggtgctataa aaaatgcaga aaacagaatt acgcaaaatg ttttacagta
    10021 ctggaaaaat gggaacgata ataattacct gttatcgctg gttacaaaat atgtgttaga
    10081 aaacaagggt atcgtttatg ctgaaaacag gatgaaagaa tattataata ccgctgctgc
    10141 acagttgaat agtttttgtg acagccctgc aaaaacagca atgcaaaaag tgcttgaatt
    10201 cgtaattgac agggaaaagt aatagtttta ttgcaaacag aaaatcgcta tgctgaatca
    10261 ttacggagaa tgggcggctc ttattgctgc tattttttgg acagttactg ccctggcatt
    10321 cgagaacgca agcaaaagga ttggatcgtt ggcggttaat ctgcttagat tattaatagc
    10381 atttgtatta ttcggggtaa tggcaatgat aaggactggc agttttttcc cagtagaagc
    10441 cagtgcacat aattggatat ggttatcgat atcaggattg attggttttg ttataggtga
    10501 tttgatgctt tttcaggctt atgttgtggt tggcgccaga gttgcaatgc taataatgtc
    10561 gcttgcgccg cctttagctg ctgtgattgg ttgggcaata atgggagaaa caatgcaaac
    10621 cacgggtcta ctgggcatat taataacatt ggcaggaatt gcgatggtaa tattaaccag
    10681 aaagaatagg gatgatgacc caaaaagcga aaaaaaaatc aaactgaaat atccggtaaa
    10741 aggtgttctg ttagctgtgg gtggtgcggc aggacaggcc acaggattag tgcttagtaa
    10801 atacggcatg cagggttatg atgtaatttc ttcaacacaa attagggtta ttactggagc
    10861 ggttggattc gtaataatat ttgcaatgat gaacaaatgg cgagccttga aagcattgaa
    10921 aaacgatccg ggttctattg tttccttggg tttgggctcg gtgttcggcc cgttcctggg
    10981 ggtaagcttc tctcttatcg ctgttaaata tgctgcggca accggagtag ctgcaagtat
    11041 tatgtcggtt gttcccgtac taataatagc accagctgta atatttttca aagaaaaaat
    11101 tacattgaag gaaattgcgg gagcaatagt tacaaccgcg ggagtggttc tgttttttat
    11161 ttgagagggt ttagaaccat gtaaccggag tagaaaggat gctagttaat atattgttgg
    11221 ccaaacgcga tgcccctata cgaaagtagg agttatttaa ccaatcgtta cccagttgcg
    11281 atgctacaat attgtaataa tagaacgcat cttctggtgt aactatgccg ccggaagctt
    11341 taatacctac tttgcgccct gtttctgcga aataattacg aatggcaata cacattgtta
    11401 aaactgcctc aggagtggca gaaacgggcg acttaccggt tgaagttttg atgaaatcgg
    11461 cgcctgattg catggcaatc caagatgctt tatagatgtt ttcgagagtt ttcaactcac
    11521 cggtttctaa tattacttta agtgtggttg caggcagcag cgacttaata agagcaattt
    11581 cgtcggaaat cgcctgatat tgctccccga aaaaattacc aagtgaaatg acaatatcca
    11641 attcatcagc acccttattt acggcgagct cgcattcggc aagtttaatg gtcaggtaag
    11701 tctgagaaga gggaaaactt ccggcaacag aagcaatttt aacatttttg tcggcaagtg
    11761 attgttttac aacaggaacc agagacgggt aaatacatat cgcagccaca ttatccatat
    11821 taaaatggct tgaaaacagg tttacttttt cggtaaaagc tttgatacct gcgttggtat
    11881 cagtggcatt aagacttgtc aaatcgatgc agtgcagtat tgtttttaaa actgttgaat
    11941 caattgattt gggtacactc gatttaattt cagtaagcat taagccgatt tgagaagact
    12001 cattaaaaac gccagaagta ttcataatta tttaagttta ggattattaa tgtgccgaag
    12061 tgagtcgcgg tttgtctttt tggtaattgt ctgcagaaaa gcctcctcaa ggttgacacc
    12121 cgtctgattg gctaaacaaa tcagcaccca taaaacatct gccatttctt cggaaagatt
    12181 aagtttttcg ttgggttttg gcgattgctc tccatagatt cgtgctacaa cacgggccag
    12241 ttccccaatt tcttcagtta gtatcgtcat attggttaat acattgaaat agcgaacgcc
    12301 atgttgtgca atccattggt caaccatttg ttgtgattcg gaaatagtca tcagaaatgt
    12361 tttaaatgca acggtagaag aagaactcgg tttcagcaat tgatttgtaa tgaaccgtaa
    12421 ttttgttttc atcgcttcta aaagggtatt ctcttataaa aatgtaataa atacccggct
    12481 caagggcaaa attgtatttg ccgttactgc aaaaaacact atcgcaatac gggtatacgt
    12541 tataaatatt ttgcagactg tcgtttaccg actgctcaat acaataggca taacctgtgt
    12601 atttactgta aaacctgaat gtgtaatcaa gcgaacaatc accttcgcca aaattaataa
    12661 taccctttgc gccaccatca acattgtcgg aagtgcatga catggtaaga aaaaatagga
    12721 ttgtcataat gctcaacaat attattttca aatcaaaacg catttacaaa tctttgtttt
    12781 tagagtcaat aataatggtt accggaccat cgttacataa gaacacattc atattagctc
    12841 caaatatacc agtttgtacg ggttttggca atagtgagtt acatttatga ataaagagtt
    12901 cgtatagggg aattgcaatg ttgggagtgg ctgccaagta ataagaaggg cggttaccct
    12961 ttttggtttt tgcatgtaat gtaaactgac ttacaaggag aatatccccg gaaatatcga
    13021 gcaatgacct gttcattacc ccggagtcat cagcaaaaat tctgagattt ataattttgt
    13081 taaccaacca gtcaacatct tcctttgtgt catcagaaac aatcccaaga aaaataacta
    13141 aacctgtagc aatttgccca accgttttat tgaggatttt aacatttgcg gtagaaaccc
    13201 gttgaataat aacacgcatg taacagaaaa tcaaatagtt aaaaattgtt ccacatgaaa
    13261 cattacacaa aaagatcttg aaatgagtaa gccgaaatat cgaataaacc tttgtcacct
    13321 atctttaagg atggaatcac tagtaatgcc atgaacgata aagtcatata aggcgacgtt
    13381 aggttgcatc ctaattcttt aaccgcatga ttcataatgc cgtattgaag tgcaagctta
    13441 tcccccgatt catccgacat taagccagca aagggaagag gaatggtata gaagttttta
    13501 ttaccgtcgt tacaagtaat accgcctttg tgagcaataa gttcatttat ggcatcgcaa
    13561 atatcactgt cgctaacccc aatggaaata atattatgac tatcatgagc cactgttgat
    13621 gctatggctc cctgctttaa tgaaaagcca tggatgaacc ctacgcttgg gcttatttcg
    13681 gagtagcggt ttaaaacaac tatttttaga atgtcgcggc ttatatcgga aaccacttta
    13741 ttgttagcaa ttaaaggttt ttcaatgagt gttccggtta acaaagatcc atcggtagca
    13801 tcaattatac ggattgatga ctctacaacc gcattcacaa caatatcgtt cgacgaaatg
    13861 ggggtattta ttgcaaaatt atttaccgct acagctttcg gcgtattaaa taatatgcgg
    13921 cctgaggaat acacgggttt accatttatg taagtttcag taacatgaaa tgtatcaata
    13981 ttgtccataa caataaagtc agcattatca cctactctaa gacacccaac attcagacca
    14041 tagtgttgta ttgtgttgag acttgaagct gcaaggagat taaaaatact cagtccttta
    14101 ttaagcccca tacgaataat tttatcaata tgccctttat ttatcaagtc gtcgggatga
    14161 gaatcgtcag tgcatagcat taccatatcg ggatgagtat ctatcagcgg acacaaagca
    14221 tcaaaattct ttgcagaaga gccttctcta ataataatct tcatgccaag atttattttt
    14281 gcaatagcct catcaaggct gaagcattcg tgatcagttg aaattccctc tgaaatatat
    14341 ttcctaagtt catcacccga aattcccgga atatgaccat caatcggttt ttgccatttg
    14401 cgggcaatgt taagtttttc aataacttca ggcatattat aaattacacc tggaaaattc
    14461 atcatttcac ccaaagcaac tataggctgt ttttcgaaga gagaatcgat gtcctgtgca
    14521 gacaggcttg caccgcttgt ttcgaagcct gtcgcaggaa cacaactggg cgcgcaaaaa
    14581 tgaaacttta atggcgtttg ctttccgtta ttgatcatga actctacacc atttttacca
    14641 attacgtttg ctatttcatg agggtcggaa acggttgcaa cagtaccgca ttttacggct
    14701 aattttgcaa actcagaagg aataagcata gagctttcaa tgtgtacatg ggcatcaatc
    14761 aatcccggag atataaatat atcgagttgt ttatcgatac gatcaatact tgtaattatc
    14821 ccattattat tgatagtaat tgcagcagga taaatctctt ttttaaaaat gtcgattaga
    14881 ttgccttcaa ttttcattgt cgctgattgt tatatatgtg tgataaagtt acgaaaatta
    14941 tgcgatttag aaaagcctat tacgatttga tttagtatag tttcacgtga aacaaaaaat
    15001 tacctaccca gataaatcag taaaacactt aggtctgatt gcgatactcc acttatccgg
    15061 gaagcctgcc caagtgtttt gggttttatt cgtttaagct tctgcctgcc ttcggttgaa
    15121 agagaagaaa tgttatcgta atcaataaac cctgataatt caatagtctc caaacgcttt
    15181 agtttttcag ccattagctt ttctcgttct atataaccgg aatatttaat cattatttct
    15241 gcggtgagca gagattcggt tttgttgtca aaatcagaaa ttagggcttg taatgatggt
    15301 atttgtgata agagagcttc aaggcttacc tgggggcggg taagaagtgt attaagttta
    15361 attttttgtt gaagaggtga tgtgtcggcc gattcaagcc atgggttaat ttccaccgga
    15421 ttgcaggaat actttcggga aaagttttca atggatgaaa tctgctgcga ttttttttca
    15481 aaacgcttca tccttgcatc agaggctaag cccagattgt gactgatttc tgtgagtcga
    15541 aaatcggcat tatcctgcct gagcaaaatt cgaaactccg cacgactggt aaacattcta
    15601 taaggctcat ccacgccttt tgtaaccaga tcgtcgatta aaactccaat ataggcctga
    15661 tcgcgcgata aagtaaattc tggtttattt tttattttca aatgggcgtt aattccagca
    15721 ataatccctt gtgctccggc ttcttcgtat ccggtggttc cattaatctg accggcaaaa
    15781 aataagttat tgatgatttt tgtttcgagg ttagcattca gttgagtcgg atcgaagaaa
    15841 tcgtactcaa tagcataacc cgggcgaaat atttttactt tttcgaaacc tttaatcttt
    15901 tgcagcgatg ataactgtac atcccatggc aaactgcttg agaagccgtt taaataaaac
    15961 tcgatactat tttctccttc aggttccaag aaaagctgat ggctatcttt atcaggaaaa
    16021 gtaacaagtt tggtttcaat gctcgggcaa tatcttggac caatcgattg gattgttcca
    16081 tcatacaggg gagagcggtc gagtcctgtt ttcagctgat catgcacttc tgcgtcggtg
    16141 ttgcaaatat agcaggaccg ctgttttaat ttatgataac catacggatg cataaatgaa
    16201 aaaggtacga tcatttcatc tcccttttgt tcctggagct gcgaaaagtc tacagtgcgc
    16261 ccgtcaattc gaacaggggt tcctgttttc attcggtcgc ctctgaatcc aaatgataat
    16321 aattgttcgg ttatgccata cgatgcactt tctccaattc tgccaccctg tatttgtgtc
    16381 tgccctatgt gcatcaggcc gttcagaaat gtgccggtgg taaggataac agatttggaa
    16441 tgaaaatgaa tccccatctg agttacgacc ccagtaattg tgctaccctc aatctgcaat
    16501 gatgtcactg aatcctgcca gaaatccaga aacggcatgt tttcgagagt atttcgccac
    16561 tccagtgaat attgcatacg gtcgcattga gctcttggac tccacattgc agggcctttc
    16621 gacatattaa gcatacgaaa ctgaatcatg gtattatcgg ttacaatacc tgcaaaaccg
    16681 ccaagcgcat ctatttcacg cacaatctga ccttttgcaa cgcctcccat tgccggatta
    16741 cacgacatct gtgctaactt ggttaaatcc atgctaataa gcaaggtgcg agacccgaga
    16801 tttgccgccg ctgctgctgc ttcgcaaccc gcatggcccc ctccaactac tattacatcg
    16861 taatgtgtta acatatcacg tttttacgct gtaaaattac acaaaagatt gacattgaat
    16921 gttgtataat tgtggatagc ttgtgctttt aggttaagaa aaacttgtgg gttaaggcga
    16981 aaagtaatca agtataatat atgtagcaag gtgtttaaaa agcaatgcca aggactaaaa
    17041 tatcatcagt ctgactcgcc tcccctttcc acaacagcca atgtgatttc aacagtaatt
    17101 caatgtcatt caggtctttg tggttttcct gagtcaaaat cccacttagg ccattcgctg
    17161 ttatttttcg tgttttattg cggtttaact ggtcggtaat accatcggta aacagatata
    17221 ttttgtcgcc ggcttgcaat gaaaacctat gttctgtagc aacgaaaaaa tcgtcattac
    17281 ccaacattaa tttatcccct gaaaatattc ttgtcccctc ttccgaaacg acaatcactt
    17341 tttgcgatgc agaaacaaga tgtataacac cgttattttt ctctatcata attatcgaca
    17401 tggcacaaga acgtaaatgt ttaatattta catctctctc ggatccaaaa aattcggatt
    17461 gcaaccgatt caaatgggtg aaaagcgtag ctggtccacc cgaagcctca tgcattgccc
    17521 tatcgaataa gcgcaaatag cttgcagcca ggaaaccccc tgcggcacca tgccccgtgc
    17581 aatcaccaac agcaatggtt aagcaatcat ctttttctgc aattctatag aagtcgcccc
    17641 caacaacctc tttgggctga taccaaacga aagccttact gcaatactct tttgggaaaa
    17701 catcttctac attttgcagt gcaagaattt gtttggcata tgaaatactt tcggtaattt
    17761 gaaggttctg actttcgata atggtatttt tttgtttgag ctttttgtta agcctgtttt
    17821 tttgaataaa aatcatggca aagagcaatg caatcagtaa gctaagaagc actaccaaag
    17881 ccaaggatat tattataatt cgttgttgcc gcaaactgcg ttcctgttcc ttctttttac
    17941 gggtaatctt atcaagttcg tatttctgtc ctgtttctac caacatttgc ttcaactcgg
    18001 catcgtaatt tgtttctctt gcagatgcat ataattcaag atactttaag gcttcgctga
    18061 tattattgtt tgccagacat gttttataag cctgattgta aaacgattct ttcagtgcct
    18121 tatcagaagc cttatcacat aaattcgctc ccatttcaat ggattggaac gactcggccg
    18181 gtttagccca gtcgagatag gtttgtgcca gtgcaatgct tacacttaac atagcgttta
    18241 tattttcgca attcgaaaac cccgcataag ccaaattata ctggcttaca gcaaaatcga
    18301 attttttcag gcgacggtat atgccagcaa tgttgttgtt aatgtatgcg agctcttccg
    18361 ttgtctccgg aagttccttt aataaattat atgcttttat ataccggctt agggcttttt
    18421 cgtattgccc aagaacggca taggcattac ctatattaga taaacaatat acgatgctca
    18481 aggtatcgcg gcaggcgtat gcataatata gcgatttttc gaaatattga atagccggct
    18541 ccagtgattg ctgttggcga taaatcacac ctatattgtt ataagctgta ttcagcctga
    18601 aattgtcatt tgctgcactg gcatagaaaa caacagtatc gtaatatgcc agcgccttac
    18661 tgagctgaga attggcctga taccacaatg ccaacgcatt gtatgcatct cccagggatt
    18721 ttaaatcgtt atgactcttt gaaatttcaa ttgcttttct tgcatatact tctgcctgac
    18781 ttccggagga gtctacattg tccatagcaa gccttatgta atagtatgaa agggtatcag
    18841 attgaaattc attcgtagct ttttgtgcat atgaaatatg tgtaaaaccc aggaaaaaag
    18901 cccatatgat tgtaaaaaaa atgtgataaa atcttattac tttcatggtt ttaaaatatg
    18961 gacctgaatg tatggttaac taagtgctaa tataataaat gcttaaaaaa ttttacgtaa
    19021 tatttttttt cgtctgtttt atttcggtga gttcttatgg gcaatattat gaagacatgt
    19081 ttgttcgtgt tccagagaaa caaaccgatg tcggtgtttt tcaagtcgtc cttcgaaacc
    19141 acaatttctt taaaaataat gagtatttta acgattatgt gtattcctat acattgttgg
    19201 gcgcatttgt tgctcctgaa ttggtgtatt ctatcagcaa taaactcatt atatcaggag
    19261 gtttttatgc acagaaatac tcaggtaaaa acgatttgaa caaaattaaa cccttatatc
    19321 gggtggtgta ttgcccaata agtgagtttt cgataaccat gggaaatatt aagggagggg
    19381 cagaacatca tctgacagaa gaaatatact gtgccgaccg tcatattatt gataataacg
    19441 aagaaggctt gcagtttatg tattcaaaag gcagctggtt tgccgattta tggcttaact
    19501 gggaccagac tgcctttcca aatgatagaa aacaagagca gctacaattg ggattttcag
    19561 gcgaattccc gttgttatca aaaaagaaaa ccgtatttac cggagtcggt cagtttttgt
    19621 gggcacataa tggtgggcag gaccttgcgg taacctatga tgtgagaact atcggatctg
    19681 tggccggggg gctgcgatat cggcagaatt tttattcaaa aatccctgcg ggtatcggtg
    19741 ccgaatgtat gtatgtagca tgtcgtgacc tgtctccgtt tttaaaattg ccctatagtc
    19801 agggctatgg cgtgcattct aaagtgtcgg catatttcag ttattttagc cttgatttgg
    19861 cacactggta cgggtaccat tatttttcgg cattcggaga acctttgttt caatcggtaa
    19921 gtcaaaaatg gggttcggca cttgctccgg aacgaaatgt ttttaccata aaagccgctt
    19981 ttaccaaaga ggtggccgat ggactgaaaa ttgttgttca ttatggtggc ttttggggaa
    20041 aagatatcga aagattcgat tatttttacg gcatgcattt gattattaat caaaatatac
    20101 tgataaataa agtaaacaga ttgaagagct tatgagaggc attacttcgg gaatattgat
    20161 attgattgct tttaccattg gttcgtgtag ttccgccgaa aaagaagtgg aatcaactta
    20221 cccggatggt aaaccactga aagttgtgta ttataaagac attgaagggg ttagggattt
    20281 ggtgaaaaca gagtattact acacaaacgg atggctcgaa agtgaaacaa attataaaaa
    20341 cggtaaaaag aacggtaaat gcctgatgta ttttacaaat gggaaagtgg catcgcagga
    20401 gtattattgc gatgatgaat tgcatggaaa atgtcattac ttttcagaaa caggtaaacg
    20461 acgttttact gccagttacg atcatggaat ttcggatggt aagtggatta tttatgatga
    20521 aaatggaaaa aaagctacgg tgcagatatt cgaaaacggc aagcttataa aacagagaaa
    20581 taaatgaaaa cacgtatcaa tgtattcatt ccagtactcc tgtttttttc gctgctatgt
    20641 ggctgcaacg gaaataatgt atccagtcaa agccggattg cggaaagaga gaaaaacgaa
    20701 aggactataa aatacgcaaa acccgcaagc gataccattg caaaaaatta tgtatgccag
    20761 gtagttgctt catttcagaa cgagcaacct caaaaggtat tttattttcg cgacagtagt
    20821 tttcaagagc ttgtttacga aatacaatat tatgaaaatg ggcaccgaaa aatggaaggc
    20881 ggatactcaa acggaaagcg ctcaggtaaa tggattgcat ggtacgaaaa cgacaccgtg
    20941 tggagctatg gttactacag aaacggttta cggcacggtg tttcggcggt atatttcccc
    21001 aatgggcaaa agtactacga taaaagttat tttaatgata cagccgaagg gaaatggact
    21061 ttttataatg aacagggaaa actcgttggc gaagcatttt atcacaatgg taaattaatg
    21121 agagagacta aatattagag agctcccatt tatttcagca tctccgattc aatagacctg
    21181 ttatacaact tccgtgcatc tttaatgaat ccgaacggaa gatcatcgat gcgtatttcc
    21241 cccttggtaa catgcccaag aactgtaaac ggaaactcaa acgacatcag aacatcaata
    21301 aaacggtttt gattttcggg aggcaccgaa gccactaccc taccctgggc ttctccaaac
    21361 aaaaacgcat cctttctaac ctcggctgct gtagttatgt cgaagccaag cttgttgggc
    21421 attgctgatt cgagtaacgc aatgtacagc ccaccatcgg aaacatcgtg aagagaggca
    21481 acaatattag tgccggttaa ggccattatt acctgaagta atttgtgctc tgtatccagg
    21541 ttgaagtgag gcggggggct atttttaact ttatgatatg aatacagata ttccgaagaa
    21601 ttgatatcgt tatatacatt tcctatgagg aaaattacat cacctttgcg ggtaaaagcc
    21661 agacctgttt gcgatctttt atcatcaagg attccaagca tcccgatagt tggggtaggg
    21721 aagacgggct ctgttttacc ggcaatggtt gtctgattat aaaatgatac gtttccaccg
    21781 gtaaccggtg tgttgaattt acggcaagcg gttcccattc ctttcactgc catcacaaac
    21841 tgccagtagg cttccggatt atagggcgac ccaaagttca agcagttggt aattgccagt
    21901 ggccgggcac cggaacatgc aacgtttcgt accgattcgg caaccgcaat catagccccg
    21961 gtttcagggt cggctttaac atacctactg ttgcaatcca ccgaaacagc cagagctgta
    22021 ttggtacctt tgatattaat gattccagca tcggaaggat tgtttgtcga catgttattg
    22081 gtccggacca tagtatcgta ttgctcataa acccatcgct tcgatgcaat attgggatgt
    22141 tgggtcagaa agtcagcgat atctttcaga tcctcgggtt ctggtatggt gtcgatattg
    22201 aaaatcttgt gattttgtac atattccggg gcttgataac ttcgctcata cacaggtgct
    22261 ccccctccaa gtaccagcga atttgcagga acttctgcaa caagcttgcc ctgataatga
    22321 aaatacaatc tatcttcatc gataacttct cctatttgct gacaattcaa gtcccatttc
    22381 gtaaaaatat cgtttacaat tttttctttt cctttctcta ccactaccag cattctttcc
    22441 tgtgattctg aaagcagaat ttcccatgcc tccatgtttt tctgcctaag cggtaccaga
    22501 ctaagatcga ttcgcatgcc agagttccct tttgccgaca tttcgctcgt ggagcagatt
    22561 attccggcgg cacccatgtc ctgcatgccg acaatggctc cggagtcgcg taattccaga
    22621 cttgcttcaa ggagtagttt ttcctgaaac gggtctccaa cttgtatcga aggaatatcg
    22681 tcggcagaat cttcggttat gtcagccgat gcaaaagttg ctccgtgtat tccgtctttt
    22741 cctgtagaag aaccaactat gaaaaccgga ttccccgcac ctttagctat ggccgaaatc
    22801 actttcccct tttccattat gccaaccgac attgcattta cgagagggtt tgtattgaag
    22861 ctatcgtcaa agaaaacttc tccgcccagc accggaactc caaacgagtt gccgtaatcg
    22921 cctatacctt ttaccactcc tttcatgagc catttggtcc ggtcattttt aatatttcca
    22981 aaacgcaatg agttcaactg agcaatcggg cgcgcgccca tggtaaaaat gtcgcggttg
    23041 ataccaccta ccccggttgc tgccccttga tagggctcaa cggcacatgg atgattatgc
    23101 gattcgattt taaacgcaca ggcaagatta ttgccgatat ctaccaagcc tgcattttct
    23161 tctccggcac ccgttaaaag gcacccacca tcgcgaggca gtgtttttag ccattttatt
    23221 gaatttttat aagaagcatg ttccgaccac atcactgaat aaatactcag ttctgtataa
    23281 ttgggaatgc ggttcaaata tttctgaata aggtcaaact caaattcgtt caagcccagc
    23341 tcctgggcgg tggctaatgt aacgggtttc tcggtcattt tatggttctt ttgtaagggc
    23401 aaatataagg aatatgcttt cttttaattg tttgaacggg tattagtttg ccaattttat
    23461 caacatattt tttaagcctg catatcaatt acttactttc ttttataaat ttgtaaacat
    23521 gctacaggag attttgttaa gccttgccct tatctttttc tttgtctttc tgataatgaa
    23581 actgaagttt ttcaggcgtt tgggtattaa aacacgggtg ttggttctgg tgtttctgct
    23641 gaagcttgtt accggcgtcg gcgtgagctt tatttacacc cattattacg acccgtggac
    23701 tgccgatatg tacaaatatt ttcacggtgg gagggctctg tattctgcta tggaagaatc
    23761 accttccgac tattttaaaa tgcttacagg catccacgcc aacgagccgc aacttacaaa
    23821 gtactatgat tatgccgatt actggtataa aaaatacaat tatggattgt acaacgacaa
    23881 tagaacgatg atccggtaca atgcttttct atgcctcatt tcgcagggga attattatgt
    23941 gaacctggta attactatat ttctgtcgtt ttgtggagct tttctgcttg cattgggctt
    24001 acacaaatgt atgcccggga aaaaccttct cctgtttttt tgcatatttc ttgttcctac
    24061 cgttttattc tggtcttctg gtttgttaaa agaaagtctc gttatgtttt caatgggggc
    24121 aatggtgtat tttctcatcc gatttaaaga attactgaag gttaaatatt tactgttttt
    24181 tattgtgact gcagtattac tctttttttc caagttttat gtgctattgg cattaacacc
    24241 gggtatggtt ttctatatgg tcgtaaagaa aaacaattac tggcgccaaa cattggtaat
    24301 gagcattatt tttggagtaa ttctgatatt gtttttcagt agcaaatcga taaccgggct
    24361 tgattttgca aagattattt cggaaaagca gaccgacttt gtgaaattcg taaatctcgc
    24421 cgaagatgcc ggcagcaatg ttgatcttcc tcaggttgat cctagtcctc aaggtataat
    24481 tagccatctg cccgttgcat attatcatag tttttttcgg ccgaatatct tcgaaggggg
    24541 ctcagtgttc tcaagaatgg ctgctttcga aaataccggt gtgatatttc tatgcatctt
    24601 gctcttaatt tatttccgaa aacccgataa gcaagaatat aaagtaatac tgttttcggg
    24661 ttctttcatt ttgattttat atgctcttat aggacttaca acaccgaacc ttggtgcgct
    24721 ggtccgctac aaaatgccgg cgatgccctt cttgatatta attatctgcc tgtgcatcaa
    24781 cacaaaaaaa atgcccctga taataaacaa gggcattcag atattatcgg atatggtaat
    24841 agaaaaaccg cttactcatc aagagcatta gcaagttctt cggttatttc gaggttatga
    24901 taaacattct gaacgtcatc gtcgtcttct aatgcatcaa taagcttcat cacttttttg
    24961 gcagtatcaa catccagagt ttttgtgtcg tttggaatgc gttgcagttc tgcactttcg
    25021 atatctactt tgagttgttc gagcttctta ttcaaatttc caaaatcctc aagcgcacaa
    25081 taaacggtaa ttgtctcttc atcaatttca atgtcctgag ctccaccgtc aatgagttcc
    25141 aattcgagtt cgtcggaatc aatttttcca ttgttcagaa tggtaaaaac gccttttcgt
    25201 tcgaaaagaa agcttaaaga accatttgtt ccgagcgagc cattgtattt gttaaagata
    25261 gaccttacgt tcgaaacaac acggttgttg ttgtctgtca tgcattcaac aaaaacggca
    25321 atgccatggg gggcataacc ctcaaatgtc gattcaaaaa gtgcgctgct gtcaccgcct
    25381 ttgttaatag ccctttcgat attgtccttg ggcatatttg cccctttggc attctgaatt
    25441 gcaacccgaa ggcgtgcgtt aaattcagga tcagcgcttc ccccgctttt tatagcgatg
    25501 gttatttctt ttgcaatccg cgaaaatatt tttgacctct ttgcatcgag agcacctttt
    25561 ttgcgtttaa ttgttgacca tttattgtgt cctgacatat gcgtttgatt ttaatgatgc
    25621 aaaataatgt gttttttagt ttttataaaa gtttttttcc ggaaactttt atcgcaaagc
    25681 taggaattaa tcgataattc tgaaaacagc aatatatcca ccgccagaag caagtcgaaa
    25741 ctttagtaca tcggttttac cgacaaactg cttttttact gtaattgctt ccgggttgta
    25801 tctataatct gcggtctctc cgtcttcata aatatcaacc tggtaatgcg tattttcgtt
    25861 aagaaagttc aaagcaatct caaattctcg acttttttca tttgtaattg cagccagata
    25921 ccatatttca cccttccggc gggtaataat aatatattcc cctatttctg cgtcaggaac
    25981 tctggtttca tcaaaagtag ttggcaactg tcttaataaa tcaagtgccg ggtgatcttt
    26041 ataattttca ggtaaatcgg cggccatttg catcggacta tacaggacca acatcagcgc
    26101 aagttgatta gcaatagtac tgtgaacagc agtgtttccg gggtcaagag agttccattt
    26161 taccctttta tcacgatgag ccgaaaggtc aatatcaaag attcctgctg tataatccac
    26221 tggtccggcc attccccggg taaaaggcaa aatacaggta tgacgtggca tatttccttt
    26281 actccaagca ttccactcca ttcccctaac cccttccgaa gtcatgagat ttggataggt
    26341 acggctcaat cccgacatta tcacaggctc gtgaacatca agcataagat gatattgtgc
    26401 tgctttgcgc ataaccatat tgcaatgctc caccatatat tgtccatgat gatattcccc
    26461 tgcaggaaca atcgccccag cgtaacctgt tttaacagca cgaattccca ttgattgata
    26521 gagcgagaat gccgaatcga gagcatactc atacgaaaca atatcacctc ctgtttcgtg
    26581 atgaccaatc agtactacat ttttcgcggc agcatattcg gcaaccttcc gaagatcgaa
    26641 atcgctgtac ggtgtcacaa agtcgaaagc ttcgggtttt ccccagtttt cccaaccggt
    26701 gttccaccct tcaatcaggc atcctcccca ttggttttct gcagaaaaat caatgtagcg
    26761 caacgcattt acggtagttg ccccgtgctt cgggccttta tcccaagtag aaatacccag
    26821 atgcatttcc caccagatgc cgttgtatac catgggttgt atccacgaaa catcgttgat
    26881 tatgcactcg tcattcaggt tgtataaaag gtttgatacc agtaagtctc cggctgtgtc
    26941 ggcaataaat acaacgcgcc aaggactatt caccggtgct cttgtcttga ctgcggtccc
    27001 atcggcccat ggaacaagat taacttgata tgcgttgttc aaattgctgt cttgtcgcaa
    27061 agtcatggtg gtatattcgg ttatggcagc ttcgtgaatg catatatatt tgccgttctg
    27121 atttttcagc gtaaaaggag tggcaacatg ctgagcgcta tcggcagctg tgtgatagta
    27181 ttctttttca agggtattgt aatcggcata gctccaccag ctttcccatg tgccatacaa
    27241 attaaaacgg gtatcttccg acataataac cacaacggag cccttacttc ctccggtgag
    27301 tatataacgg aaagcaattc cgtcgtcaaa acagcgaaat tcgacattta cacgtgattt
    27361 gccatttttt ttgcgaagat ttactgtaat actgttataa ttgttttgta ccgaggagct
    27421 aattccccac acagggctcc aattttcaga aacctgcttt tcgcttttcg taataaaatc
    27481 gaacgtattg aggctgtcat tctgcaatac aatacccagc cttgaagcgc caagcagtgt
    27541 gtccccttta tgttgcactg tataaaatgc atttcctgct gtgtcgagaa tgaaacaaat
    27601 ttcgttaatt gcattaggag atgaaacttg tgtctgatag ctttgcgagt acactccgga
    27661 tggtaatacc aaccaaacaa acaacattat atctaaaagt atatattggc gaaatgtttt
    27721 catgcgttgt ttttatgcaa aaatagaaat tctggccggt aataatcatg aaatcagtaa
    27781 caaaccggaa agagacagta acatcataag gtttttcaaa aaaattagta tctgccaaag
    27841 ccaagtgcta tggtaatgct attaaatttt tccatatttt taaagaaaat ttataaactt
    27901 ttgcaagtaa taagaaataa tgtatatttg cactcccaaa ataagggcaa atttaatagt
    27961 aacaatagtt taaagatatg gcacatcatc agtccgcaaa aaaaagaatc agacaaacag
    28021 aagctcgtcg ggtgcagaat aaatatgcag ccaaaacaac ccgcaacgct gtaaggaagt
    28081 tgcgttcagt taccgacaaa aaagaagcag aaaagctttt tcccgaagtt gcatcaatgt
    28141 tggataaact ggcaaaaaat aatgttattc atcataagaa agcagcaaac ctgaaatcga
    28201 gtctggccgt tcagctgaat aaaatgcaat aaaccatcca aaatatttac ggagccggct
    28261 tattgccggc ttttttttat atttatgctt tgttagagta aatgagaaat tggaatagca
    28321 gacaaataca aaaaataaat ctactaattg ttacaggggg ttaccgatga aatcaaaatc
    28381 cattaaaaac tgggctgtcg atgatcgccc cagagaaaaa atgatcaata aaggaactgt
    28441 ttctctgagc gatgctgagc taattgccat tctgctacgt accggaaacc gcgataaatc
    28501 ggcagtggag ctggcacggg agattctgga tgccaacagc aataatctca acttattggg
    28561 aaaacaagat atttcacatt ttaaaaagtt taaaggcata gggcaggcta aggcggtcga
    28621 aataattgct gcccttgaac tggggcgtcg gagaaagctc gccgaaattc cagaatataa
    28681 aagcattaca agcagcaaag atgctttttc aatgtttgaa cctatgctgg cagatctgag
    28741 ccatgaagaa ttctgggtgg cattgctcaa ccgggcaaat cacgttaccg atatcttcaa
    28801 aatcagtcag ggtggtacag cggggacggt gatggacgta aaaatcatta tgaaacgcgc
    28861 cctcgaaaaa ctggctcacg gaattctgat atcgcacaat cacccaagtg gtaacaataa
    28921 gccaagtgac gcagaccgtc agataaccca caaattgcgc gatgcagcaa agcttttcga
    28981 tattaccctg ctcgatcaca ttattgttgc caataagcaa tattacagct ttgccgacga
    29041 aggaatgttg taactaaaat ttgtaagtat atttgcccga tgatacaagt gcttgtaaat
    29101 aacgaaacac ctcgtgtaaa atacgcatta tcgcttgttt tcaggcaggt gctacatact
    29161 gagttcgaga ttattacgaa ccttgatcag gtgaatggtt tttgcatcaa ctattcggga
    29221 aacccagctg agggcctatt gaaaatatgg ccacatccca tattgaatga ggacgaaata
    29281 ccagcttata accccgttgt cagtagctgg aacgaactcc ccgtgttttt ttgctctccc
    29341 gatggctttg atattccttt cgatgttttt gccgcttcat tttgggtaac aacccgttac
    29401 gaagaatatt ctacctccga aaccgacttt atgggtcgtt acaaagcttc ggcatcgcat
    29461 gctgctcgta acggatacct gaaaaaacct gtggtgaacc tgtgggcggc cgaacttaaa
    29521 aaactaatat tgcaaaaaga ctcttcggtt gtatttggcg aaatggacta ccgttttgtg
    29581 ccaaccatcg atgttgacag catgtataaa tacaagtata aaggatttgt gcgaaataca
    29641 ggagggtttt tcagggattt gtttaccggg aggttcagta attgcagcga aagactaaat
    29701 gtgttgctta atcgtaaacg cgacccctgg gattgtcttg gcgaaataga gaagattcat
    29761 atcgaaaatg gtatgctgcc aatgtatttt atattagcag ccaaacggag taagtacgac
    29821 aaaaatattc cagcaaactc atcacaattt gcaaaaaatg tgttggccct acagcataat
    29881 tgcaggttgg gaattcaccc ctcgtttgca ggacattgcg atgaaaacgc atggaagaat
    29941 gaaaagaaaa ccctggaagg cattctcaga acacccgtat atgaggcaag agtgcacttc
    30001 ctaaaactaa catttccaga cacataccaa aagttattgg aattgggaat acaattcgat
    30061 ttcaccatgg gttaccccga aactacaggg ttcagagcgg gtatttgcag tccgtttttc
    30121 tttttcgacc tgactgaaaa ccgtgaaaca gaattgtgtg ttgtgcccct gggaatgatg
    30181 gatgtaaccc tcagcaaata tatgaaaatg aatactgaag aagccaaaat tgctgtagct
    30241 gaaattgtga aagaaatcag acaggttgag ggagtatttg taagcctatg gcacaacgaa
    30301 agttttgggg aagatgtgga taatgcgcac acagaaaatt tgtacagata tcttttaaaa
    30361 gtagccaaat agttgaaaat taactattta aaacataatg caattgattt taatgcatgg
    30421 gatgaatgca ttagcagctc tgtgaaccca cgtatctatg ccacctcttc atacctgaat
    30481 atagtcagtc ccggatggga tgcacttata actgatgatt atcgctgtgt gatgccacta
    30541 acccataaaa agaaattcgg agtctcatat atttttcacc cgccactgac gcaacagctt
    30601 ggtatctttt cgatagagtt caccaatacc gaaacaacca agaaatgtat cgaggccatc
    30661 ccccgaaaat tccgcttggt ggatatgccc atgaatacgg caatgccaat ggatcaatcg
    30721 ctttattctg aacagggtat tacacatcat ctttcattac agcccggtta taccgaacta
    30781 cactcgggat attcagagaa tacatgccgc aatattgcta gggcggttaa aaatcagctg
    30841 catgtaactc aaagctccga tttcggtgtg ataagctata tgattaatgc aggctgtcag
    30901 ccttgggttg gagggcttgg aaaggaaacc ataaaggatg ttgaaaaaaa actgttgcag
    30961 ctgcttataa atggaatagg tagtttgttt accgttttgg attcaaataa cgaagtattc
    31021 gctgctgcag tgttttcgca atatcgtgga tatattacct acctttttgg tatttcgtct
    31081 gataccggaa aagataaaag ggccatgttc ctggcgtttg atactataat ccacaccaat
    31141 tcaggcagta acataatcct cgatttcgag ggctcttcca tcgaaggtat tgccagattt
    31201 ttcagaggat ttggggcgac tcccgtatat ttccccaaac tgaaaatcaa caatttgccg
    31261 gctgctatgc ggtattttaa aaaataaaaa aaaatagtat tattactttt tcaaaattaa
    31321 ccggacatga agattgtaaa cctcggggga aaaggaacgc tgctcgacca gtatctggct
    31381 gagataagag acgaagctat tcaatgtgag cgcatgcgtt tcagaaaaaa catgcagcgc
    31441 gctgccagta tttttgccta tgaagtgagc aaagccctgc cctttaccga tgaggaaatt
    31501 cagacgccgc tgggtgttgc cattgttccc acgatgaagg agcagccggt tatagcatca
    31561 gtactgcggg cgggtatccc catgcacgaa ggggtactcg attttttcga tcacgccgat
    31621 tctgccttca ttgccgggta ccggaaatac gaaaacaacg atactttcga tattcagatc
    31681 gattatatta cttctccttc tatcgaaaag cgactgctga taatctgcga tccgatgctg
    31741 gctaccggtc agagcatggt gttggcatat cgcggtttgc tgaaacacgg aaaacctcgt
    31801 catacgcata ttatttcgct tattgccagt aaggaaggag tggagcacct gcaaaaatat
    31861 cttcccgaca acgaggtaac aatatgggtg ggagctatcg acgatgaact tactgttaaa
    31921 tcgtatatcg ttcccggact gggcgatgcg ggagatcttg cttttggatt gaaggaataa
    31981 gttgtttttc gcccggatgc aaccttttct attttcccgg cgtatattta cagtacgatt
    32041 aatatagcat atgaaaaaat tcgttatagg tttgctgttt ctaatatcaa ttgtcacatt
    32101 ttggagttgc gagaatgcaa ttaatattac ccagccgacc attctggttg aatcggcgca
    32161 gctttcagtt ttcgattccg tcagcatttc ggctaccgaa aacgccgata tcgacggtaa
    32221 cagagtggta cccaattatt acgaatggaa aattctggat tattccgaaa acacactata
    32281 caccgatttc cctgatgaga ataacattac ctgggtgccc gatagtgccg gaaactttat
    32341 tataagtgtt aaaataggat acgatggcaa taaaagtgtt acaacgctgc gtgaagttac
    32401 tgttacagaa tgccctgctt cgatgcaaca gcggctgatt ggtcattgga ccggcactgc
    32461 tgaagctatg tttggagcca cctggaatat tga
//
LOCUS       DAOOQP010000019        31790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1630_length_31790_cov_6.641059, whole genome shotgun sequence.
ACCESSION   DAOOQP010000019 DAOOQP010000000
VERSION     DAOOQP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31790
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_31790_cov_6.641059"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1211)
                     /locus_tag="PLK75_03585"
     CDS             complement(48..1211)
                     /locus_tag="PLK75_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38357.1"
                     /translation="MIKQIVKNYMRFFPVVLLITVLIGTFNSCKKITGTTRCHLDTPI
                     VIGTSIVVKLSIEELSSELHLQYGFCYSTTNVTPLLSDPKLVHNSISDTTTLVDTLKL
                     LEPGMRYYIRGFVLDGDEIIYSSNIVEATTIGLPALGNVNVVSANPGLIEFSCNVTSD
                     GGGMITERGVCWSGMSSPTVENNNKIVCGSGTGEFSGNISGLPVATSYYMRAFATNIS
                     GTTYSEEVNFKSLGVGDYYRGGIIAYFLQPGDNGYIAGEYHGIIAAENDYNFYSWSNG
                     VLVITEATAMELGYGNANTTLIVDVQGSGTYAAEHCFNLVQGGYDDWYLPSLSELQVL
                     YNNRYAIGFIEQAIYWSSSEHDLVEAYVIHFGNGARVKQHKHYQYRSRAIRSF"
     gene            complement(1220..1960)
                     /locus_tag="PLK75_03590"
     CDS             complement(1220..1960)
                     /locus_tag="PLK75_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38358.1"
                     /translation="MKTIIYALIIGLTVCLTSCKIHRELATVSSGGGDVEKVIVKNNQ
                     IDGVTENQTLFTYKKNDQTIMEEVDPIDFVLVKKEKAKVYWGINKERYAVVNVNKYFY
                     VYSGGKVFLKIILAPFTYFTSLFWIKAREITPIEYETCNYDDLCKGHTYDKQILIEAK
                     IWCKRDRITDTVTIVESFPDAIKFKDYKVKVKRGDVEKVIHEVKTENSQSYHVFKIVG
                     DDNMFEGKNKVWIIMDVAITPTEEYYKN"
     gene            2234..3811
                     /locus_tag="PLK75_03595"
     CDS             2234..3811
                     /locus_tag="PLK75_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38359.1"
                     /translation="MKFRLLLIIICLVSQIAYSQSDSLNGYKVFYYPGGQKSSEGFLR
                     EGKPDGYWRSYYENGFVKSEGTRNNFLLDSIWKFYYNTGDLHQVISYRNDQKNGYTLT
                     YTYEKTNNNDSAYFLKSKELFLNGIRQGESFYYYPNGNLQFKVYYRNDKRHGKGKELN
                     ESGQVISLFEYFNGYLIENTKINRLDANNIKQGRWIDFFQHGEIKTDVTYLNGKYHGY
                     YREYNEKGELLKEIRYLNGEPVTRDVEEELRVKADVRTVYHENGKPKYIGAFLEGKPV
                     GIHREYNTDGFLIMAQQYDDFSVLQAQGLFDDTGNKTGKWTLFYESGKILGTGNYKND
                     LRDGEWYYYYENGSTEQKGNYKNDKPDGYWVWYYPSGKILREENYLDGKREGDVAEYD
                     ENGNYISKGSYFDGLKTGDWYYNVGDHTEEGAFENGYKNGVWKYYYPDKRISFEGEFR
                     GGDPIGAHYYYYADGTVALSGKYKTGKKHKKWKKFNSDGSLFLVYTYKNGKIVEIDGK
                     KIKEADAGIYKEEQEHF"
     gene            3826..4863
                     /gene="ruvB"
                     /locus_tag="PLK75_03600"
     CDS             3826..4863
                     /gene="ruvB"
                     /locus_tag="PLK75_03600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOY38360.1"
                     /translation="MDEIIHSQDKNNISITDLEIESQLRPDSFHDFNGQQKIIDNLIV
                     FVEAAKMRSESLDHVLLHGPPGLGKTTLANIIANELGVGLKISSGPVLDKPGDLAGLL
                     TGLQRNDVLFIDEIHRLSPIVEEYLYSAMEDYRIDIVIDKGPAARSVQIPLLPFTLVG
                     ATTRSGLLTSPLRARFGITCHLEYYDADILKSIILRSAAILKIAITDNAATEIARRSR
                     GTPRIANALLRRVRDFAQVKGTGSIEINITRHALDALNIDQFGLDQMDNKILTTIVDK
                     FSGGPVGLKTIATAVGEDEGTIEEVYEPFLIMEGFIKRTPRGREATELAYRHLNRFMK
                     PEQKGLFDNSV"
     gene            4907..7246
                     /locus_tag="PLK75_03605"
     CDS             4907..7246
                     /locus_tag="PLK75_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOY38361.1"
                     /translation="MNKLLILFFTLWFSAFLQAQNNYPNVINYTINLEITDFAGASVS
                     GSTVLQFSADGNNLGVISLDLLALDIDSIVSDDFDVISYTYNDTLIEITPSVEITPTD
                     TFYMAVYYHGHPVIDPSGWGGFYFTADRAFNLGVGFEDVPHNYGRVWFPCVDNFTDRA
                     TYNCNITVLENHMAICGGELLNVTEVENGKHTYQWFLSDEIPTYLASVAVGNYSLVEH
                     TYHGIEGDIPVELYVYPTSVGNVAGTFINLDTIISIFEHKFGPYRWQRVGYVAVPFNS
                     GAMEHATNIALPTFAITGNTSYQDLIAHEFAHHWFGDLITCSTAADMWINEGWATYSE
                     SIFREFLFGRINMLNYRRDYHSEVLRYAHIEDGGFLSLNAIPIDKTYCTTAYEKGASV
                     AHALRGYLGDELFFNAITALLEEYRFNAISSEQMRDFLTNYTGVDLTDWFDGWVFSPG
                     FPHYSIDSVTTVTNSKSYDVTVYVKQKLRGKTEFINSNRVPITFIDENFNDTTCWVYF
                     DGETGNASFQLTFNPVFSFCDYYETISDATIDQHMFLKNTNTQNLAKCYIRIKPLTLS
                     ADSVFIRITHNWVPPDTMKTKIPGIFLANNRYWTVEGNFVANSTMKAEFQYGNAANSS
                     VTGYLDNDFINNSLDSLVLMYRSSKAVEWQIVESTNSTVVKRITTENLLSGEYALGIK
                     NWDAWIKVPEVTKNNNSVMKVSPNPAQSIIRISFDKFVSGILRITDISGNIVYSASIE
                     NKNLFTLNLCHLTDGIYFIQFIDTDNSVDCSKLIINKTF"
     gene            7272..8564
                     /gene="tyrS"
                     /locus_tag="PLK75_03610"
     CDS             7272..8564
                     /gene="tyrS"
                     /locus_tag="PLK75_03610"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOY38362.1"
                     /translation="MNFVNELEWRGMIHDIMPGTKEMFDKELVTAYVGIDPTADSLHI
                     GHLVSIMMLKHLQVNGHKPIVVLGGATGMVGDPSGKSNERNLLDENTLQHNEDCIRKQ
                     IARFLDFSENSPSGALLVNNYSWMKNYSFLEFVRDIGKHITVNYMMAKDSVKKRLSGD
                     SIQGMSFTEFSYQLLQGYDFVHLYREHGCKLQMGGSDQWGNITTGTELIRRIEKADAY
                     ALTCPLITKSDGGKFGKTEEGNIWLDPNKTSAYKFYQFWLNTSDEDAAKYIKIFTLLN
                     KEEINSIIAKHSEAPHIRLLQKKLAEEITCMVHGRAEYDNSLSASEILFGKSTELGVF
                     SLNEQTILSVFEGVPQFTFEKNLLKSGIPVVDLLTDFCSVFPSKAETRRTIQGSGLSI
                     NRQKISDLNIVIDVNYLISNKFIIVQKGKKDYYLLIAK"
     gene            9038..9973
                     /gene="nadA"
                     /locus_tag="PLK75_03615"
     CDS             9038..9973
                     /gene="nadA"
                     /locus_tag="PLK75_03615"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOY38363.1"
                     /translation="MHTETQDMISQILKLKTEKNAVILGHYYINSELQDISDFVGDSL
                     QLSQQAAKTKADIIVFLGVYFMAETAKILSPDKKVLIPDPASGCSLADSCNAFEFEKY
                     LKNYPGYTVVSYVNTSAEIKALSDIIVTSSNAMKIINTLPKDEKIIFAPDRNLGNYIN
                     SITGRNMLLWNGACHVHEQFSVEAIINYKKQYPDALVLAHPECEKTVQVLADFIGSTN
                     ALLSYSIKSESKTFIIATESGILHQMKKNSPEKLFIPVPSIDSSCGCNDCSFMKLNTI
                     EKLYKCLLNEYYEVLVNEEISRKARTSIERMLLLS"
     gene            9989..11161
                     /locus_tag="PLK75_03620"
     CDS             9989..11161
                     /locus_tag="PLK75_03620"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOY38364.1"
                     /translation="MKKFIISIICILVLGAYFTTLNAQETENISFVSADIGTSYVLKS
                     YSRLPDLKPNFNFGVHFGKQLNNVKSWHKNYNYPGVGAGLFFARNNNKIIGDVIAIYP
                     EASFIVKNKNKHQCRITSALGIAYFSNPYHPVTNNLNMLIGSHVTAIAKADVCYRFLY
                     SPRLAIQISLGLIHFSNGHVKLPNIGINMPQVSAGATYNFNDELRRPPLTKTKYNDIK
                     TKAIIRYGNGFHQFGESAKPYGGPVYKNTVVSIGAAHNYSAVAYLSFGVNFTYYSSFY
                     HFLLDQEVFGEKSLFYESCMITIYGAHEFLFGKLGVYTEFGIDIYKPFYRKYALYYDN
                     KLSMDEIIKSFNSNKLGLQYHFFKPLNSGFDIALGVYIKSNFAQADFIECALNFAF"
     gene            11206..11997
                     /locus_tag="PLK75_03625"
     CDS             11206..11997
                     /locus_tag="PLK75_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY38365.1"
                     /translation="MKKLLFLAAIAMFVTGVNAQEGAFAGGHFAYNSTWLMNPQVFDD
                     GAGQNPDVSWGYYYGLVLGYNFKDNMGVEVDFNVNKLTQKYDGEIDVWLTDDEAGYKS
                     HTTMNTFDIPVMMKFGENSYFELGPLFHIVNKVNYTRDFNNDDEIGDVGVYKDLFYLC
                     RNDERNVAEEFNSFGFGVAMGFGSDIPLVNDALYLNFGVRFNYILTDLGGVNGLGFNI
                     DGYDGYHNNTDFVPEDNQDDENEKDRFHTNPLYGGFKIGLKYRFN"
     gene            12058..12777
                     /locus_tag="PLK75_03630"
     CDS             12058..12777
                     /locus_tag="PLK75_03630"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOY38366.1"
                     /translation="MKIHYNDILDSTNNEARRLLQQIDCKHIHIVVAKYQSAGRGQSG
                     NTWASNYGENILMSLIIGNNELPASAFFHLTRIASVSVIDYLSEKNIAAEIKWPNDIL
                     VNKAKIAGILIENNIEGLYITQSIIGIGLNVNQTDFPNFGIPAISMKTILEKSFSPDE
                     EISHLAGHIIRNRQKTVSEINRLYHKRLFMLGKTLTFRKNNREFQARLCGVSDDGKLL
                     LQLEGMIEKSFDFKEIEWVIS"
     gene            complement(12781..13920)
                     /gene="hflX"
                     /locus_tag="PLK75_03635"
     CDS             complement(12781..13920)
                     /gene="hflX"
                     /locus_tag="PLK75_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOY38367.1"
                     /translation="MTNSSILQNTNAVLVACESNEQSHEKTIDNLEELAFLSETAGFN
                     TLRIFTQKLIKPNGVTYVGSGKLDEIADFIRNNPVGFAVFDDELSPTQLRNIEDVLQV
                     RILDRTHLILDIFAMRAKTSYAKAQVELAQYQYLLPRLTRMWTHLERQRGGIGLRGPG
                     ETEIETDRRVIRDKISALKDQLVQIDKQMSVQRKNRTKIIRVALVGYTNAGKSTIMNL
                     LSKSDLLAENRLFATLDTTTRKVVINNLPFLLSDTVGFIRKLPHQLIESFKSTLDEIR
                     ESDLLVHVVDVSHKNYQEQIITVRNTLAELNLQDKSVLLVFNKIDNIDDKISTDDLQK
                     SWIAYENTPCIFISAKQGLFIDEFRGLLFELVKKIHFDRYPYNAI"
     gene            complement(13974..14672)
                     /locus_tag="PLK75_03640"
     CDS             complement(13974..14672)
                     /locus_tag="PLK75_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY38368.1"
                     /translation="MQIKKSPKADLERQKMMFLQIGLVAALAVMLVAFEWKDSEKALS
                     TLGNLAVEDVMEEDVENTFREEKPPEPEPEPEPQQTVVEELNIVDNDVDVEDDFDFNS
                     ESDQNDKSNTSISDFSGDEVVEEPEPISFAVVEEKPTFPGGDAELMGFIAKNTKYPEI
                     AKENGIQGRVFVQFVIASDGSVTKVTLARGVDPYLDEEAIRVVKLLPKWTPGKQRGKA
                     VPVTFVVPINFKLY"
     gene            complement(14803..15492)
                     /locus_tag="PLK75_03645"
     CDS             complement(14803..15492)
                     /locus_tag="PLK75_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOY38369.1"
                     /translation="MKTKKSPKADLEKKKTIFLEIGLIVSLSLILYGFELGSSGKIVN
                     ANYGNTVKIIDEDIIPVIPDRPKPQPVEPEKPVARTEINIVDNNHHQDSVNIFNNENI
                     DDPVDPLIFTWETDTASKDVVEVIEFYKVEVKPLFPGGDEALLKYLAENCKYPAEAVE
                     NSVQGTVYISFIIDEKGRVTNLTIARSIHPVLDKEAIRVVSTLPDWTPGRQRDIAVPV
                     SYIIPIKFKLY"
     gene            complement(15649..18444)
                     /gene="polA"
                     /locus_tag="PLK75_03650"
     CDS             complement(15649..18444)
                     /gene="polA"
                     /locus_tag="PLK75_03650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOY38370.1"
                     /translation="MAMSSKVFLLDAYALIFRSYYAFFKNPRINSKGMNTSAVFGFTT
                     TLLEILKKEKPTHIAVVFDPPAPSFRKEIYPAYKANREATPEVIKMSVPFIKQIIEAL
                     NIPVIEIDNFEADDTIGTLAKKLAGQGCKVYMMTPDKDYTQLVEENILIYKPGRSGSE
                     VEIIGVAEVKSRFSVQHPDQVRDILALWGDSSDNIPGAPGIGEKTAKKLLEKYGTVEN
                     LIQHAAEIYGKVGENLLQFRDQILLAKQLVTIRTDVPVSARLSDLELTEPKLEKLSSI
                     FADLEFRTLADRFFSEYKNVTHQGQPDLFSTTVRDNKNTDAELFSSSGLSHKIITPIE
                     ADYQSVDTAFLRKKLIDELLSSGEVSFDIITSGLNPLNDDILGISFCFSEKTAFFVLM
                     PDNKPDTLKVLKEFIPFFENKNILKIGQHLKFDLLFLKNHGIEASGPFFDTMIAHYLI
                     EPEQRNNLDDLAVKYLNYASAHLEEIMGKKRFLTTDIRSLPLDEITKYGAEDAFLTFK
                     LKAVFEKELTERNIKNLFYDVEMPLVAVLTDMEFTGICIDTKQLSDYSVNLKKELSEV
                     EDRIYRIAGNRFNIASSKQLGAVLFEQLKITDKPPLTKTKQYATGEEILANFANAHPI
                     VGEILEYRSISKLLSTYVEALPYLVSEKTGRIHSTFNQTTAATGRLSSANPNLQNIPI
                     KEERGRLIRKAFIPCSNNSLFFSADYSQIELRILAHMSGDKNMIEAFRYHNEDIHTAT
                     ASKIFRVTKEEVTREMRSKAKTANFGIIYGISAFGLSQRLNINRADAKNLIENYFQTF
                     PGVKEYIDKSIHLAHERKYVETIMGRRRYLPDIDSRNANIRSFAERNAINAPIQGSAA
                     DIIKLAMINIFNNIAANRLMSKMIIQVHDELNFIVPEIEKDLLKSIVVADMEQVVNLS
                     VPLKVECGFGKNWFEAH"
     gene            18606..18689
                     /locus_tag="PLK75_03655"
     tRNA            18606..18689
                     /locus_tag="PLK75_03655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18640..18642,aa:Leu,seq:caa)
     gene            18725..19159
                     /locus_tag="PLK75_03660"
     CDS             18725..19159
                     /locus_tag="PLK75_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOY38371.1"
                     /translation="MTNHPNPEKQRQFDQHYLEMAQIWAKNSYCKRKQVGALIVKDRM
                     IISDGYNGTPSGFENDCEDENFVTKPYVLHAEANAITKVARSGNSSEGATLYITTSPC
                     IECAKLIIQCGIQRVVYLDEYHNRDGIELLKKAKIEVIKFKV"
     gene            19156..20802
                     /locus_tag="PLK75_03665"
     CDS             19156..20802
                     /locus_tag="PLK75_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY38372.1"
                     /translation="MMLSPRNRLFLPLIIAISVIAGMLLSLALFRNQTKEVLGFKLPK
                     KDKLSTIIDYVKSEYVDEVNVDSLVELAIPNFLRQLDPHSVYMTADEAKAVNEPLEGN
                     FEGIGIQFSVNNDTILVVDIIHGGPSEKLGLKAGDRIITINDSVVAGVGIKSDNVVKL
                     LKGPRGTTVKVGILRKGRDKLLHFNIIRDKIPILSIDAAYMIDDKTGYIKISSFSKNT
                     HTEFVESAFKLHQAGMKNLILDLRGNSGGYLDAATNIVDEFLKGGKMIVYTEGRSRPR
                     NSVYATDKRAVCVDVNLAVLIDEFSASASEIVAGAIQDNDRGWIIGRRSFGKGLVQEP
                     TIFKDGSMLRLTTARYYTPSGRCIQKNYSGGYESYYDELMERYLHGEFQVADSIDQND
                     SLQYKTSGGRLVYGGGGVMPDIFIPIDSVLFNPTLRLLSDFGLAYSFALQYTDKNREL
                     FSGISSVDSLIMVLDSRYTMSEFGTYIRKNKHYISYFNWNKYMPYIKSQVYGYIVRNV
                     FDDNAFYGYINKIDNTYLQAVKTLESNESVTGTQNSPSHK"
     gene            complement(20768..22090)
                     /locus_tag="PLK75_03670"
     CDS             complement(20768..22090)
                     /locus_tag="PLK75_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY38373.1"
                     /translation="MNLKKYINNITGLQAFQLLRFGMLLLISIVFTKTKLGVGEIGIY
                     ETFLLIAGGVSFFWISGIIQSFLSFYKNPGGENGSKSSDIFNTALILTILSILAALFI
                     FFSRSFLAELLNLTGGQIPYLKILLMYVVLSGPVNLIEYIYLVKNKSGWIIKYGLISL
                     SLQFFCVTTPVLLGFDLGYGLYGLVLVNIIRFIWLMVLVYKYSRLRLSKDYLKKHLYI
                     GSPLVLSLLLSGSAQYIDGFLVSNYYNEATFAVFRYGARELPFVILLANAFSNAFIPE
                     FSNNYQLSDSLLSLKNRSLRLMHFLFPVSICFLLLSKWLYPIVFNQSFTESAEVFNIY
                     ILIIISRMVFPQTILIGRQKSGIIMFASFVEIVVNVFFSVLFIRTIGMLGVAYATIVA
                     YYLEKILLILYLRYKMHIHLKDYTAVRWFLIYSLLTLAIYVMVSFVFR"
     gene            complement(22116..22727)
                     /locus_tag="PLK75_03675"
     CDS             complement(22116..22727)
                     /locus_tag="PLK75_03675"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOY38374.1"
                     /translation="MFSGIVEETAKIVKIEQEQSNYHFYLTCSFAGDLHLDQSVSHNG
                     VCLTVVEVNKSDKVYKVTAIQETIDKSNLGLLSVGDEVNVERSMTMDRLLDGHMVQGH
                     VDQTAECIEVAEADGSWYYTFRYDSSKGNITVEKGSVSVNGVSLTVVNSKNDRFSVAI
                     IPYTWQITNFHNIKAGTIVNLEFDIIGKYIQKIMAGYLENFKK"
     gene            22999..24012
                     /gene="pseB"
                     /locus_tag="PLK75_03680"
     CDS             22999..24012
                     /gene="pseB"
                     /locus_tag="PLK75_03680"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOY38375.1"
                     /translation="MLTNKSVLITGGTGSFGKKFVETILNRYPEVKRLVIYSRDELKQ
                     FEMAQMYPSAKYPQVRFFIGDVRDYPRFKRACQGIDVIIHAAALKQVPAAEYNPDEFI
                     KTNINGAQNVIDSALGSQVKVVVALSTDKAAAPINLYGATKLVSDKLFIAANNIKGSR
                     DLRFSVVRYGNVMGSRGSVIPFFMDKARRGEVIPITDKEMTRFNISLEEGVEMVLWAI
                     ENAIGGEIFVPKIPSYKIEAVAKAIAPNAKLEYVGIRPGEKIHEEMITTSDSFYTIDL
                     GKYYAILSAGAHKEKYLDYYKAKEVPRGFQYNSGENTEWIDENQLRELIVKYVDPNFK
                     PVL"
     gene            24009..25160
                     /gene="pseC"
                     /locus_tag="PLK75_03685"
     CDS             24009..25160
                     /gene="pseC"
                     /locus_tag="PLK75_03685"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOY38376.1"
                     /translation="MKPIPYGRQSITDDDIKAVVETLKSDFLTQGPRIAAFESAFAEY
                     TGSKYAVAVANGTAALHLCTLALGVKKGDKVITTPITFAASANCVRYCGGEVVFADIN
                     PETYLLDIESVRHILENNNSGTIKGIIPVDFAGRAVDIVAFRKLADEFGLWIIEDACH
                     APGGYFNYGKNSQVSCGSGQYCDLAIFSFHPVKHIAAGEGGMITTNNEELYNKLILLR
                     THGITKLPQLLNENHGGWYYEMQELGFNYRLTDFQAALGHSQLKRADEGIKRRKEIAK
                     NYFEALQNLPFIDGQSGVVEGHAYHLYIIEVERRKEMYDYLKSNNIFAQVHYIPLHLM
                     PYYRKLGWKPGDFPNAENYYQKCLSLPMYPTLSDVEQDYVIDTITKLEK"
     gene            25157..25993
                     /locus_tag="PLK75_03690"
     CDS             25157..25993
                     /locus_tag="PLK75_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOY38377.1"
                     /translation="MIESKIGLGTVQFGLNYGISNTVGKTDSSEVGRILEVARMNGIN
                     TIDTASGYGESEKILGISDVKEFHIVSKYKPPVEGFSIKNQLNNSLNQLRIKALYGYL
                     AHRPMDLLQHREQWEEMLLFREMKLVDKIGFSCNETAELELLLGKNFIPDIIQVPYNY
                     FDKRFEPYFLQLKSKHVEIHTRSSFLQGLFFVPADKLHPYFERVKPLIGALQCKYGNN
                     LSRILLGHCLKNPSIDKVIIGVNNVTQLNENLRAGYEGDILPDLNESIPDNIVMPSQW
                     KL"
     gene            25981..26787
                     /locus_tag="PLK75_03695"
     CDS             25981..26787
                     /locus_tag="PLK75_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY38378.1"
                     /translation="METVNKYAQIFDLSEKTAIVTGGYGHLGSGIVRALLAFNADVVV
                     AGRSIEKYEAVFSEAERKNLSFEQIDITNSQSIANCFEVVVEKYGTIDILINNAHTAK
                     GNSPENMNDEDWAYTMDGVVGSVHKAIREIMPVMKNQGFGKIVNIASMYGLVSPDFRL
                     YEDDNCEMYRNPPHYGAAKAAMIQLTKYYAALLAKYNIQVNAVSPGPFPKAKIQTDNP
                     LFVERLKKKNPMNKIGEPVDLAGVIVLLSSRGSSFITGQTLQVDGGWTIW"
     gene            26781..27515
                     /locus_tag="PLK75_03700"
     CDS             26781..27515
                     /locus_tag="PLK75_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOY38379.1"
                     /translation="MVKDNITIILQARMSSERLPGKVMKKISGIPMIGIQIERLRKSG
                     IPIVVASSELPENDVLEDYLTSIEIPIFRGSELNVLDRFYHAAKKYHAEYVIRITGDN
                     PLIDGTFLGNTISGIQNFGKRSYFSIGRSNTFPLGFSFELFSFELLEEAYNNAQSPKE
                     KEHVTPYIHQNVPGNIIIKTLYREPTRNEYRLSVDTNEDLILVKTLIENYDCRNKPMD
                     EIIDILDKNPELVAINNRITQKKWNE"
     gene            27672..28520
                     /locus_tag="PLK75_03705"
     CDS             27672..28520
                     /locus_tag="PLK75_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOY38380.1"
                     /translation="MLKTYFFIPANVQKFIEKAAGIPSDYIVFDLEDSVAFNEIDEAL
                     GNLKCIDIRKNYFVRPALFAKNKLDVDKDMLHSLIDIGFTNYIIPKFSDVLQLAKLKE
                     IFTVSSQYDYHTFKFVILVEHPAGLLFLKEAVQSKQLNISALALGNYDYCNAMGMKHN
                     LENLYYARQIILNTARAYDLSAIDIVTLDYKDREAFCSEVLNGFSMGFDSKFLIHPMQ
                     LEQIHSIKYYSDEEVAEARELYPKILEMMQNKTSIVKLNGKIFEKPHINRIINIINWN
                     NRYGSK"
     gene            28507..28968
                     /locus_tag="PLK75_03710"
     CDS             28507..28968
                     /locus_tag="PLK75_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOY38381.1"
                     /translation="MEANRMGHYFEEFEAGQVYYHCQSKTIFESDNNIFSLLTMNHHP
                     VHTNIDYCSRQQHGQVLVPGTLVFCLAVHLTVMDISGQAIANLAYENIDHLAPTFIND
                     TIYSRTVILETKESQSKPDRGIIYVETIGYNQNNVDVIKFRRKVLIKKKPQ"
     gene            28970..29866
                     /locus_tag="PLK75_03715"
     CDS             28970..29866
                     /locus_tag="PLK75_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOY38382.1"
                     /translation="MNEKKFIMRSLMFVPGHNEKLQISAAKSDADVILLDLEDSVLPE
                     SNKQLARDTIVKNVSSGMFKDYAVFIRINERQSGFLLDDVMQTTIPGVDGYLFSKTYN
                     AQDIVFFDKLLEAIEYKKGMPVGTFKIIPILETSSSIIKADEIATASSRNVAIGFGSE
                     DFVSDLQGIRDFDKAVSLFSPRAWVAMVARTYNLIPIDAAYIKIHDEEGLEKHCQMGR
                     TLGYAGMWTLHPKQNAVPNRHYSPSEEEIKEAYEILRLDEEARSMGKGVAIINGSFIG
                     PPLVVKAKHIIERVKLIQSKQK"
     gene            29863..30516
                     /locus_tag="PLK75_03720"
     CDS             29863..30516
                     /locus_tag="PLK75_03720"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38383.1"
                     /translation="MKLIIIGGYGNGTVIAQIAEEINKVKHTWDILGFLNDFEEGYIN
                     EYPILGKVEHDVAQKYLADPDVYFFYALISIKLNFNFLYKLHDLNIPLERFANLIHPN
                     AVVSKAATIGHGVSIQAFSIVGPNATMGNFIQVYGHATVGHNCTVDDYAYVTSNSIVG
                     AHVHLKQGAFLGVNSTTLDRVTIGEWSIVGQHSNVIRDVPDYTKVVGNPARIIGEVK"
     gene            30513..31550
                     /locus_tag="PLK75_03725"
     CDS             30513..31550
                     /locus_tag="PLK75_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38384.1"
                     /translation="MKILFRTDGNSQIGFGHLIRCVSIVNEIRKQSELHSPEIEFLGS
                     FSDAAQEVLLNEKILFQLAETHEEFNLLSENISIFRPDVLFIDRVYNYSEEEIKALKK
                     NCRIIMMHYISPGIRTCDHYILPSEHTPQNLIDESGMMTGKGQVHMGADYIVLNHKLS
                     GLRRNTYTPAQHIQIAITTGGSDPKQVLLKILRILMVYSWENVRFKVLYGLSIAFGEE
                     LEKLKKVLPAYFTVIPYNEKELIDADLAICTFGITTYELLYLGIPVISVSHAESNALG
                     SKTLAQKHHDIIADLGLAENLTSDALITCIKSLIQDADKRIYMAESGKLLVDGRGVSR
                     VAEIIINSMNQ"
     gene            31547..>31790
                     /locus_tag="PLK75_03730"
     CDS             31547..>31790
                     /locus_tag="PLK75_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOY38385.1"
                     /translation="MIKQQKTFIIAELSANHNQDFNLAVKTIEAMAEAGADAVKIQTF
                     KPGSLTLDLDTGYFAPRTEGLWKGYTPWKLFQEAAMP"
BASE COUNT        10056 a         5862 c         6235 g         9637 t
ORIGIN      
        1 aacaatcaaa acgtgtctct agttcaatac gattacaccc aattaattta aaatgaccta
       61 atagcacgac ttctgtattg ataatgctta tgttgtttaa ctcttgcgcc attcccaaaa
      121 tgaattacat aagcctctac cagatcatgt tctgatgaac tccaataaat agcttgctca
      181 ataaatccaa tagcgtatct gttattgtac aatacttgca attctgataa agacggtaaa
      241 taccaatcgt catagccccc ctgaacgagg ttaaaacaat gttcggcagc atacgtccca
      301 ctaccttgca catcaacaat caacgttgtg tttgcatttc catagccaag ctccattgca
      361 gtggcctctg taataacaag caccccattt gaccacgaat aaaagttata atcgttttca
      421 gcagcaataa ttccatgata ttcccctgca atatatccat tatcgcctgg ttgtaaaaag
      481 tatgcaatta taccacctcg ataataatcg ccgaccccta gtgatttaaa atttacttct
      541 tcgctgtatg tagtaccaga aatattggtt gcaaaagctc tcatataata ggatgtcgca
      601 acaggaagtc ctgaaatatt accggaaaat tcgccagtac cactaccaca cactatctta
      661 ttattatttt ctactgttgg gctcgacatt ccactccaac aaactccgcg ttcggttatc
      721 ataccccctc cgtcgctggt tacattacaa gagaattcaa taagaccagg gttcgctgaa
      781 acaacattta catttcccaa ggcaggcaaa ccgatggttg ttgcctcaac aatatttgag
      841 ctataaatta tctcatcacc atccaaaaca aaacctctga tgtaataacg catacccggt
      901 tcaagtaact ttaaagtgtc aaccaaagtg gtagtatcgg agatagaatt atgaactagt
      961 ttaggatcgc tcagcaaagg agtaacattt gtggtgctat agcaaaatcc atactgtaaa
     1021 tgcagttcac tacttaattc ttcaattgat aatttaacaa caatacttgt tccaataaca
     1081 attggagtat ctaaatggca ccgtgtggtt cctgtaatct ttttacaact attaaatgta
     1141 ccaatcaaaa ctgtaatcag taatacaact ggaaaaaatc tcatataatt tttcactatt
     1201 tgtttaatca aatttaattt caatttttat aatattcctc cgttggtgta atagcaacat
     1261 ccataattat ccacactttg ttcttacctt caaacatatt atcatcgcca acaattttaa
     1321 atacatgata tgattgactg ttttccgttt tcacctcatg aattactttt tcaacatcgc
     1381 ctcttttaac ttttactttg taatctttaa attttattgc atcaggaaaa ctttcaacta
     1441 tcgtcacagt atcggttatc cggtcgcgct tacaccagat tttagcttca ataagaattt
     1501 gcttatcata tgtatgtcct ttacacaaat catcgtaatt gcaagtttca tactctatcg
     1561 gagtaatttc ccttgcttta atccagaata atgacgtaaa ataggtaaag ggagctaaaa
     1621 ttattttaag aaaaaccttc cctccgctat atacataaaa atacttatta acgttaacaa
     1681 ccgcatacct ttccttattg ataccccagt acaccttggc cttttctttt tttaccaaaa
     1741 caaagtcgat gggatcaact tcttccataa tagtttggtc atttttttta taagtaaata
     1801 atgtctggtt ttcggttacg ccgtcaatct gattgttttt tacaatcact ttttccacgt
     1861 ctcctccccc tgagctcaca gttgcaagct ccctgtgaat tttacatgac gttaggcaga
     1921 cagtaagccc tattatcaaa gcataaataa ttgttttcat acgcctgtat ttttatgatt
     1981 aatgttaatt ggttcttgct acaaacttat taatattgtt tttaaatatc aagatttaag
     2041 catttaatgt ttataaaatt gcattattta aatttattcc attttatttt tataccaaac
     2101 cttgtgattt aataattttg acttgtttaa gatgaaacga tttttaaatt ttggcaccta
     2161 aaaaggtact tattgcatca taatccataa ttgtacttaa ctttgtacgt ttatatgaga
     2221 acgatttgta gtaatgaaat ttagactgct gctaataatt atttgtttgg tttcgcagat
     2281 tgcatattcg caaagcgatt cgctgaacgg gtataaagta ttctattacc ccggcggcca
     2341 aaagtcgagt gaaggttttc tgcgcgaagg aaaacccgat ggttactgga gaagttatta
     2401 cgaaaacggc tttgtaaaat cagaaggtac acgaaacaat tttttgcttg acagcatttg
     2461 gaagttttac tataataccg gcgatcttca tcaggttata tcgtaccgca acgatcagaa
     2521 aaacggctat acgctaacgt atacttatga aaaaacaaat aataacgatt cagcgtattt
     2581 tttaaagtcg aaagaattat ttctaaatgg aatcaggcag ggagagtcgt tttattatta
     2641 tccgaacggc aatctgcagt ttaaagttta ttaccgtaac gataaacgac acggaaaagg
     2701 caaggagttg aatgagtcgg gtcaggtaat cagccttttt gaatatttca acggttatct
     2761 tatcgaaaac acaaaaatca atcggctcga tgccaataat atcaaacagg gaagatggat
     2821 cgattttttt caacacggcg aaataaaaac cgatgtcacc tatctcaacg gaaaatacca
     2881 tggttattac cgtgaataca acgaaaaagg tgagctttta aaagaaatcc gctatctgaa
     2941 tggcgaaccg gttacacgcg atgttgagga agaactaagg gtaaaagccg atgttagaac
     3001 cgtgtatcac gaaaacggaa aacccaaata cataggtgct tttctcgaag gcaagcccgt
     3061 tggaattcac agagaatata atactgatgg cttcctaata atggctcagc aatacgatga
     3121 tttttcggtt ttacaggccc agggcttatt cgatgatacc ggaaataaaa caggaaaatg
     3181 gacattgttt tacgaaagtg ggaaaatatt gggaaccggt aattataaaa acgatttacg
     3241 cgatggtgaa tggtactatt attacgaaaa cggctctaca gagcaaaaag gaaattataa
     3301 aaacgataaa cccgatggat attgggtatg gtattacccc tcgggaaaaa ttctgcgcga
     3361 agaaaattac cttgatggaa aacgcgaagg cgatgttgct gaatacgacg aaaacggaaa
     3421 ttatatttct aagggcagtt atttcgatgg attaaaaacc ggagattggt actacaatgt
     3481 tggcgaccat accgaagaag gtgcttttga aaacgggtac aaaaacggtg tctggaaata
     3541 ctattacccc gataaaagaa ttagtttcga aggagaattt aggggtggag atcctattgg
     3601 agcgcattat tattattatg ccgacggtac tgtggcgtta tcggggaaat ataaaacagg
     3661 caaaaagcat aaaaaatgga aaaaattcaa ttctgatgga agcttgtttc ttgtttatac
     3721 ctataaaaat ggtaaaattg tggaaattga cggaaagaaa ataaaagagg ccgatgctgg
     3781 tatatacaaa gaagagcagg agcattttta acgaataaaa ccggtatgga tgaaataatt
     3841 cactcacagg acaaaaataa tatcagtatt acagaccttg aaatagagag tcagttacga
     3901 ccggactctt ttcacgattt caacggtcag caaaaaatca ttgacaatct gattgttttt
     3961 gttgaagctg caaaaatgcg ctcagaatcg ctcgaccatg ttcttttgca cgggcctccc
     4021 ggtctgggta aaactacact tgcaaacatt attgcaaatg aacttggggt aggattgaaa
     4081 atatcatccg gtccggtact tgataaaccc ggcgatttgg cgggtttact aaccggcctg
     4141 cagcgcaacg atgttctttt tattgacgaa atccaccgct taagtcccat tgttgaagag
     4201 tatctgtatt cagctatgga ggactatcgt atcgacattg taattgataa aggcccggct
     4261 gcccgttcgg ttcagattcc gttgttacct tttactctag tgggagccac aacccggtcg
     4321 ggattgctta ccagtccgct cagggctcgt tttggaatta cctgtcatct cgaatattac
     4381 gatgccgata tactgaaaag cattatactc cgttcggcag ccatcctgaa aattgctatt
     4441 accgataatg cagctacaga aattgcgcgc aggagccgtg gtacaccccg tattgccaat
     4501 gccctgcttc gtcgtgtacg cgattttgct caggtaaaag gtaccggttc aatcgaaatt
     4561 aacattaccc gtcatgctct tgatgccctg aacatcgacc agtttgggct ggatcagatg
     4621 gataataaaa tactcacaac tattgtagat aagttttcgg gtggtccggt tggacttaaa
     4681 acaattgcca cagccgtagg tgaagatgaa ggaacaatag aagaagtgta tgagccattt
     4741 cttattatgg aaggttttat taaacgtacc cccagaggac gcgaagctac agaactcgct
     4801 tatcgtcacc tgaaccgttt tatgaaacct gagcaaaaag gcctttttga taattcggtt
     4861 taagagttgg acaataaaaa caattactgg aataaaatat gtctatatga ataaacttct
     4921 catacttttt ttcactcttt ggttttctgc ttttttacag gctcagaata actatccaaa
     4981 tgttatcaac tatacaataa accttgaaat aaccgatttt gcaggggctt ccgtatcggg
     5041 tagtactgtt ttacaatttt ctgcagatgg caataacctt ggtgttattt ctctcgattt
     5101 attagcgttg gatatcgatt caatagtttc tgatgatttc gatgttataa gctatacata
     5161 taatgatacc cttattgaaa taactccctc tgttgaaata actccgacag atacattcta
     5221 tatggcggta tattatcacg gtcatccggt tattgatcct tcgggctggg ggggttttta
     5281 tttcactgcc gacagggctt ttaatttagg cgttggcttt gaggatgttc ctcacaacta
     5341 tggcagggtt tggtttcctt gtgttgataa tttcaccgat agggcaacct ataactgcaa
     5401 tatcaccgta ctcgaaaatc atatggcaat ttgcggaggg gaattgctga atgttacaga
     5461 agttgaaaac gggaaacata cataccaatg gtttttaagc gacgaaatac ctacctatct
     5521 tgcatcggtt gccgtaggca attactcttt ggtggagcat acctatcatg gtatcgaagg
     5581 agatattccc gttgaattgt atgtttatcc cacctctgtg ggtaatgtgg caggtacatt
     5641 tataaatctc gatacaataa tttcgatttt tgaacataaa ttcggaccat atcgttggca
     5701 acgggtagga tatgttgctg ttccatttaa ttcaggagct atggaacatg ctacaaatat
     5761 tgctttaccg acgtttgcca ttacaggaaa tacttcgtat caggatctta ttgcacacga
     5821 gtttgcccac cactggtttg gagatttaat cacatgcagc acagcagccg atatgtggat
     5881 aaacgaaggc tgggcaacat actcagaatc tattttcaga gaatttttgt ttggccgtat
     5941 aaatatgctg aattaccgtc gcgattacca ctccgaagtg cttcgttacg ctcatatcga
     6001 agatggcggc tttttaagtc ttaatgctat tccaattgat aaaacctatt gtacgacggc
     6061 ttacgaaaaa ggagccagtg tggcacatgc acttagaggt tatcttggcg atgagttatt
     6121 ctttaatgcc attactgcgt tattagaaga atatcgtttt aacgccatat cgtcggaaca
     6181 aatgagagat tttttaacca attataccgg tgttgacttg actgattggt ttgatggctg
     6241 ggtgttttct ccggggtttc cacattattc gattgactca gttacaacgg ttaccaactc
     6301 caagtcgtat gatgtaactg tatatgtaaa gcaaaaactc agaggaaaaa ccgaatttat
     6361 taattcaaac cgggttccta taacttttat tgacgaaaac tttaacgaca caacctgctg
     6421 ggtttatttt gacggtgaaa cagggaacgc ttcgtttcaa ctgactttta accctgtgtt
     6481 ctcgttctgc gattattacg aaaccatttc ggatgcaact atcgaccagc acatgttttt
     6541 gaaaaatacc aatacacaaa atctggcaaa atgctacata cgcattaagc cacttacctt
     6601 atcggctgat tctgtattta ttcggattac tcataattgg gtgccacccg acacgatgaa
     6661 aacaaaaata ccgggaattt ttctggcaaa taacaggtac tggacagttg aaggcaactt
     6721 cgttgcaaat agtaccatga aagcagaatt ccaatatgga aacgctgcaa actcatcggt
     6781 tacgggttat ctggataatg attttatcaa taacagcctc gattcactgg ttcttatgta
     6841 ccgttcttcg aaagctgttg aatggcaaat tgttgaaagc acaaattcaa ccgttgtaaa
     6901 acgaattaca accgaaaatc tgcttagcgg tgaatacgct cttggtataa aaaactggga
     6961 tgcctggatt aaggtaccgg aagtaacaaa aaacaacaat tctgtcatga aagtatcccc
     7021 caatccagca caaagcataa tcagaataag cttcgataaa tttgtaagtg gaattctcag
     7081 aataaccgat atatcaggta atatcgttta cagtgcatct atcgaaaata aaaacctgtt
     7141 tacactgaat ctatgccatc tgaccgatgg aatatatttt attcagttta tcgacacgga
     7201 caattctgtt gattgtagta aattgattat aaacaaaact ttttaaaact cagacccata
     7261 atatcatcag tatgaatttt gtaaatgaac tggaatggcg gggaatgata catgatatca
     7321 tgcccggaac gaaagaaatg tttgacaaag aattggttac cgcctatgtt ggcattgacc
     7381 ctactgccga ttcacttcat attggtcacc tggtaagcat tatgatgcta aagcatttgc
     7441 aggtaaacgg acataaaccg attgttgtat taggtggcgc aaccggtatg gttggcgatc
     7501 cttcggggaa gtcgaatgaa agaaatttgc ttgatgaaaa cacactacaa cataacgaag
     7561 attgtatcag aaaacaaatt gcccgatttc tcgattttag cgaaaattct ccttcaggag
     7621 cattgctggt aaataattat tcctggatga aaaactatag ttttctggaa tttgtaagag
     7681 atattggaaa acacatcaca gtgaactaca tgatggcaaa agattctgtt aaaaaaagac
     7741 tatccggtga ttctattcaa ggtatgtcgt ttaccgaatt ttcgtatcag ttgcttcagg
     7801 gatatgattt tgtgcatctt tatcgtgaac atggatgtaa actgcaaatg ggtggcagcg
     7861 atcagtgggg aaatatcact accggcaccg aactcatacg acgtatcgaa aaagccgatg
     7921 cctatgcact tacctgtcct ttgataacca aaagcgacgg gggtaaattc ggaaaaaccg
     7981 aagaaggaaa tatctggctc gatcccaata aaacctctgc atataagttc tatcaattct
     8041 ggttaaatac cagcgatgaa gatgctgcaa agtatataaa aatatttacc ctacttaata
     8101 aggaagaaat caattctatc atcgcaaagc attccgaggc acctcatatt agattgcttc
     8161 agaaaaaact ggctgaagaa atcacctgta tggttcatgg tcgtgcagaa tatgataatt
     8221 cactatcagc atcggagatt ttgtttggta agtcaacaga attgggagtt ttttctttga
     8281 atgaacaaac tattttgtcg gtattcgaag gagtacctca atttacgttt gaaaaaaatt
     8341 tacttaaatc gggaattcca gtagtggatt tacttactga tttttgttct gtttttccgt
     8401 cgaaagccga aacacggcgt acaatccaag gtagtggttt aagcattaac agacaaaaaa
     8461 tcagcgattt gaatattgta attgatgtga attatcttat ttccaataaa tttattattg
     8521 tacaaaaagg taaaaaagac tattatctgc ttattgcaaa atagttataa acatcagtta
     8581 atttattagc aatcaattaa ttatattaat tactttttgt aatattctat aaatcagaat
     8641 actcgataat taactaattt tcatttttcc tgtgaatttt aagttacata ttctgaaaat
     8701 aattacttgc ttcatgattt aaacctgtgt atgttttttt ttatcattaa tgcaataata
     8761 tttcattaga aaaagtgaga atagcattaa caggaataac taattttaaa gtcaaatatt
     8821 tattaaatta taacatgaat aaacgaaatt aaccattgtt aattaaaatt ataacctatt
     8881 aaatgtcaga ttacaaaaac gaatttcgct gtttacaagt atgaattact ttttataagt
     8941 tacatttgca actaaaacca aaatttgttt taaacgaatg aacagacaat aataatagat
     9001 atttcattta taaatttaat caattaaaat aatattaatg catactgaaa ctcaagatat
     9061 gatttcgcaa attctgaaac ttaagacaga aaagaatgca gttatactcg gacattacta
     9121 tattaactct gaattgcagg atatctccga ttttgtaggc gatagtcttc agttgtcgca
     9181 gcaagctgcc aaaaccaaag ccgatattat tgtttttctt ggagtgtatt ttatggcaga
     9241 aactgcaaag attctttcac ctgataaaaa agttcttatt ccggatcctg cctcgggttg
     9301 ctcattagcc gattcatgca atgcctttga atttgagaaa tatttaaaaa attatccagg
     9361 atatacagtg gtgagttatg tgaacacatc tgccgaaata aaggcacttt ccgatattat
     9421 cgtaacttca tcaaatgcta tgaaaatcat caatacgctt ccgaaagatg aaaaaataat
     9481 atttgcacct gatagaaatc tgggaaacta tataaatagc ataacaggaa gaaatatgtt
     9541 gttatggaat ggtgcatgcc atgtgcatga acaatttagt gtagaagcca ttattaatta
     9601 taaaaaacag tatcccgatg ctttggtttt agcccatccg gaatgtgaaa aaactgtaca
     9661 agtacttgcc gactttattg gttctaccaa tgcacttctt tcgtattcaa tcaagtcgga
     9721 aagtaaaaca tttattatag ccacagaatc aggaatcctg catcaaatga aaaaaaattc
     9781 cccggaaaaa ctatttatac ccgtaccatc tatcgacagc agttgtggat gtaacgattg
     9841 tagttttatg aaattaaata ccatcgaaaa actgtataaa tgcttgttaa atgaatatta
     9901 cgaagtactg gtaaacgaag aaatcagtcg aaaagcacgc acatctatcg aaagaatgtt
     9961 attattatcg taatctgcaa ccaatggcat gaaaaagttt attatcagca tcatttgtat
    10021 tttggtttta ggtgcatact ttacaacatt aaatgctcag gaaaccgaaa atataagctt
    10081 tgtttcagct gatattggta cttcttacgt actaaaatct tattcacgtt tacccgactt
    10141 aaagcccaat tttaatttcg gtgtacattt cggaaaacaa ctcaacaatg ttaaatcatg
    10201 gcataaaaat tacaattatc caggtgtagg tgccggatta ttttttgcta gaaacaataa
    10261 taaaatcatt ggcgatgtaa ttgccatcta tcccgaagct tcgtttatcg ttaaaaacaa
    10321 aaacaaacat cagtgtagaa ttacttcagc gctgggtata gcttattttt cgaatcccta
    10381 ccacccggtt acaaacaatt taaacatgct tattggctct catgtaacgg ctattgccaa
    10441 agcagacgtc tgttaccgtt ttttatactc accccgcctt gcaattcaga ttagtctcgg
    10501 attgatacac ttttcgaatg ggcatgttaa attacctaat ataggcatca atatgcctca
    10561 ggtatcggca ggagccactt ataacttcaa cgacgaattg cggaggcctc ctttgacaaa
    10621 aacaaagtac aacgacataa aaaccaaagc aattattcgt tatggtaatg gttttcatca
    10681 atttggtgaa tcagcgaaac cgtatggtgg tccggtgtac aaaaataccg ttgtttcaat
    10741 aggtgcagca cacaactatt ctgcagttgc ttatctttcg tttggcgtta attttactta
    10801 ttacagcagt ttttatcatt ttttacttga tcaggaagtt ttcggtgaaa aaagtctgtt
    10861 ttacgaaagt tgtatgatta caatttatgg tgcacatgag tttttgtttg gaaaactggg
    10921 tgtttatact gaatttggaa tagatatata caaacctttt tacagaaaat atgcactata
    10981 ctacgataac aaactcagca tggatgaaat aataaaatca ttcaacagta ataagctggg
    11041 cttacaatat cattttttta aaccactgaa ttctggcttc gatattgcac ttggtgttta
    11101 cattaaatca aattttgctc aggccgattt tatagaatgt gcattaaatt ttgctttctg
    11161 attaatttta atttattttt gaagggttaa ttcaaatcta taattatgaa aaaactatta
    11221 tttcttgctg caatagcaat gtttgttacc ggtgttaatg ctcaggaagg agcttttgcc
    11281 ggaggtcatt ttgcgtataa ttcaacatgg ctgatgaacc cacaggtttt tgacgatggc
    11341 gccgggcaaa atcccgatgt ttcatggggt tattattatg gattggtgtt gggttataat
    11401 tttaaagaca acatgggagt agaagttgat tttaacgtaa ataagctaac ccagaaatac
    11461 gatggcgaaa tagacgtgtg gctgacagat gatgaggccg gttataaatc gcataccact
    11521 atgaacactt tcgatattcc ggtgatgatg aaattcggag aaaatagtta tttcgaactt
    11581 ggacctctct ttcacatagt taataaggta aattatacaa gagattttaa taacgacgat
    11641 gaaataggtg atgttggtgt gtataaggat ctcttttatt tgtgcagaaa tgatgaaaga
    11701 aatgttgctg aagagtttaa tagttttggg tttggtgtag caatgggttt cggatctgat
    11761 atccctctgg tcaacgatgc cttgtatctg aattttggtg ttcgattcaa ctatatttta
    11821 acagacttgg gtggtgttaa tggtcttggt tttaatatcg atggctacga cggctaccat
    11881 aataatacag atttcgtacc tgaagataat caggacgatg aaaatgaaaa agacaggttt
    11941 cacaccaatc cattgtatgg cggctttaaa ataggactta aataccgatt taattaacat
    12001 gtgcttataa atgaaaaagc tgcctaaaag cagctttttt agtatctgat aaccgtcatg
    12061 aaaattcatt acaacgatat tctggactca accaacaacg aagcccgccg gttgctgcaa
    12121 cagatcgatt gtaagcatat tcacattgta gtggctaaat atcagtcggc cggaagaggg
    12181 caatctggca atacctgggc aagtaattac ggtgaaaata tactgatgag ccttataatt
    12241 ggtaataatg aacttccggc atcggcattt tttcatttaa cacgcattgc ttcagtatcg
    12301 gtaatcgact atttaagtga aaaaaacata gccgctgaaa taaaatggcc caatgatata
    12361 ttggttaata aagcaaaaat tgccggcata ctgatcgaga acaatattga aggactgtat
    12421 attacccaat caatcattgg aatcggttta aatgtaaacc aaacagattt ccccaacttc
    12481 ggaatacctg caatttcaat gaaaacgata ttggaaaaat cgttttcacc cgacgaagaa
    12541 atatctcatt tggccgggca tattattaga aatcggcaaa aaacagtttc tgagatcaac
    12601 aggctttacc acaagcggtt atttatgctg ggaaaaacac ttacgtttcg caaaaacaac
    12661 agagaattcc aagccaggct ttgtggcgtt tccgatgatg gaaaactact gcttcagttg
    12721 gaaggaatga ttgaaaaaag ctttgatttt aaagaaatcg agtgggtaat ttcgtgatgc
    12781 ctaaatagcg ttgtaagggt atctgtcgaa gtgtattttc ttaaccaact caaacaataa
    12841 tcctctgaat tcatcgataa ataaaccttg ttttgctgat ataaaaatgc agggagtatt
    12901 ttcgtatgca atccacgact tctgtaaatc atccgttgag attttatcgt cgatgttgtc
    12961 gattttatta aaaaccaaca atactgattt atcctgcaaa tttagctctg ccaatgtgtt
    13021 tctgacagtt attatctgtt cctgatagtt tttatgtgaa acatctacta catgtaccag
    13081 caaatccgat tctctgattt cgtcgagggt cgatttaaac gattcaatta attgatgggg
    13141 aagtttacgg ataaacccta ctgtatcaga tagtaaaaat ggtaaattgt tgattacaac
    13201 ttttcgggtg gtggtgtcaa gtgtggcaaa caaccggttt tcggcaagca aatcagattt
    13261 actgagcaaa ttcatgattg ttgatttccc ggcattggtg tatcctacaa gtgcaacacg
    13321 aataatttta gtcctgtttt tccgctgtac cgacatttgc ttatcaattt gtaccagctg
    13381 gtctttcagg gcacttattt tatcacgtat tacccgcctg tcggtttcga tctcggtttc
    13441 tcccgggcca cgcagtccga taccacccct ttgccgttca agatgtgtcc acatacgtgt
    13501 aagacgtggt aaaaggtact gatactgagc cagttcaacc tgagcttttg catacgatgt
    13561 ttttgcccgc attgcaaaaa tatcaagaat caggtgtgtt ctgtctaaaa ttctaacctg
    13621 caaaacatct tctatgtttc tgagctgcgt tggtgaaagt tcatcatcaa acactgcaaa
    13681 acccactgga ttatttctga taaaatcggc tatttcatca agcttacccg aaccgacata
    13741 ggtaacacca ttgggtttaa taagtttttg ggtaaaaata cgcaaggtat taaatcccgc
    13801 tgtttccgat aaaaaggcca gctcttcgag gttatcaatg gttttttcat gcgactgttc
    13861 attactttcg cacgccacca gcacggcatt agtattttgg agaatgctag aattggtcat
    13921 ttcaataaaa aaccccgatg aactgtcggg gtttgcaatg cttatggttt atattaatac
    13981 agtttaaaat tgataggaac cacaaatgtt accggaacag cttttccgcg ctgctttccg
    14041 ggtgtccatt tgggcaatag tttcactacc ctgattgctt cttcatcgag ataaggatca
    14101 accccgcgtg caagggtaac ttttgtaaca ctgccatcgg aagcgataac aaactgcaca
    14161 aaaacacgac cttgtattcc gttttcttta gcaatttcgg ggtatttggt attttttgca
    14221 ataaaaccca tcagttctgc gtcgccaccg ggaaaggttg gtttttcctc aacaacggca
    14281 aatgaaattg gttcaggttc ttctacaact tcatcaccgg agaagtcgga gatgctggta
    14341 ttcgatttgt cgttttgatc gctttcggag ttaaaatcaa aatcatcttc cacatctaca
    14401 tcgttgtcaa caatgttcag ttcttcaaca accgtttgct gtggttctgg ttccggttcc
    14461 ggttctggcg gtttttcttc acggaaagta ttttcaacat cttcttccat aacgtcttca
    14521 accgctaaat tccccagagt gctaagggct ttttcgctgt ctttccattc gaatgccaca
    14581 agcattactg caagagcggc aaccagacca atttgtaaaa acatcatttt ctgacgttcg
    14641 agatctgctt ttggtgactt tttaatttgc atagggcaaa atttttaagt gggtaaattt
    14701 ataatatttt atttgactct tcaaaattat tttattttaa taacgctcat ttgatagtta
    14761 tattgtgcct gaagccttca taaatctact gttttcgcaa ttttaataca gtttgaattt
    14821 gataggtata atatagctta ccggaacggc tatatcgcgt tgcctgcccg gagtccagtc
    14881 aggcaaggtg cttacaaccc tgatggcctc tttatcaagc acaggatgta tgctacgggc
    14941 tattgtcagg ttggttaccc tgcctttttc atcaataata aaactgatat atactgtgcc
    15001 ttgaacgctg ttttcgacag cctcagccgg gtatttacaa ttttcggcca aatattttaa
    15061 taatgcttca tctccaccgg gaaacagggg ttttacctca actttataaa actcaattac
    15121 ttcaacaaca tctttactgg cagtatcggt ttcccaggta aagataaggg gatctaccgg
    15181 gtcgtcgata ttttcgttgt tgaaaatatt cacagagtcc tgatgatggt tattgtctac
    15241 gatgttgatt tcggtacgtg caacgggttt ttccggttcc acgggctgag gtttgggtcg
    15301 gtcgggtatc actggaatga tgtcttcatc tattattttt accgtattgc catagtttgc
    15361 attgacaatt tttcctgaag aacccaattc aaaaccgtaa agaatcagac ttaacgaaac
    15421 aatgaggcca atttccagaa aaatggtttt ctttttttcc agatctgctt tgggtgattt
    15481 ttttgttttc atgaccggct gattttaatg aataatattc cgaactcaac tgtcttttgc
    15541 cggttgatga aaacacaata gataatccat aaatttttta cgaaaaattt aataggaatt
    15601 cgttattgtt tataataaat tgtaaatcaa ttatataagt acgttgtact aatgtgcttc
    15661 gaaccagttt tttccaaaac cgcattcaac tttaagcgga acactgagat ttactacttg
    15721 ttccatatca gcaacaacaa tagatttcag taaatctttt tcgatttcag gcacaataaa
    15781 gttcaactca tcgtgtacct ggattatcat ttttgacata agcctgtttg ctgcgatgtt
    15841 attaaatatg ttgatcatag ccagtttaat aatatcggcg gcagaaccct gaatcggagc
    15901 attaatagca ttgcgttcgg caaaactcct tatgtttgca ttccggctgt cgatatcggg
    15961 cagataacgc cggcgcccca ttatggtttc aacatatttg cgttcatggg ctaaatgaat
    16021 agacttatcg atatattctt ttacgcccgg aaaagtctgg aaataattct cgatcagatt
    16081 ttttgcatct gcgcggttga tattcaaacg ttgcgacaaa ccaaatgccg aaataccata
    16141 gattatacca aaattggctg tttttgcctt tgaacgcatt tcgcgcgtta cctcttcttt
    16201 ggtaacccga aatattttcg atgctgtggc agtatgaata tcttcgttat ggtatcgaaa
    16261 tgcttcaatc atatttttgt cgccactcat atgggctaaa atccgaagtt caatctgcga
    16321 atagtcagcc gaaaaaaaca gactgttatt actgcaagga ataaaagctt ttcgtatcag
    16381 gcgtcctctt tcttccttaa taggaatgtt ttgtaaattc ggatttgccg aactcagcct
    16441 tcccgtagct gcggtggttt ggttgaatgt actgtgaata cggcctgttt tttctgaaac
    16501 aaggtatgga agtgcttcaa cataagtact gagaagttta ctaatggagc gatactccaa
    16561 tatttcaccg actatgggat gggcattggc aaaatttgca agaatttctt cgcccgtagc
    16621 atattgcttt gtttttgtta atggaggctt gtcggtaatt ttaagttgtt caaataaaac
    16681 tgcccccagc tgtttcgacg aggcaatatt gaaccggtta cctgcaattc gatatattct
    16741 gtcttctacc tccgagagct cttttttcag gttcaccgaa taatcagaca attgctttgt
    16801 atcaatacat ataccggtaa attccatatc ggtaagtacg gcaaccagag gcatttctac
    16861 atcataaaac aaatttttta tgtttcgttc tgtaagctct ttttcaaaaa ctgctttaag
    16921 cttaaacgtc aaaaaagcat cttcagcacc gtattttgtt atttcatcga gaggcagact
    16981 tcgaatatcg gttgttaaaa aacgtttttt acccataatt tcttcaagat gggcagaagc
    17041 atagttcaaa tatttaacag ccagatcgtc gagattattg cgctgctccg gttctataag
    17101 ataatgggca atcatcgtat cgaaaaaagg cccggaagct tcaataccat gattttttag
    17161 aaatagtaaa tcaaatttca ggtgttgacc tatttttaga atatttttat tttcgaaaaa
    17221 tgggatgaat tctttcagaa ctttaagtgt atccggctta ttgtccggca ttaaaacaaa
    17281 aaaggccgtt ttctcagaaa agcaaaacga tatgccgaga atatcatcgt tcagggggtt
    17341 taaaccggag gtgatgatat cgaaactaac ctccccggaa cttagtaatt cgtcaataag
    17401 ctttttacgt aaaaatgcgg tgtcaaccga ttgatagtca gcctctatgg gtgttatgat
    17461 tttgtgagaa agaccagaag atgagaataa ttctgcatcg gtgtttttat tgtcacgaac
    17521 agttgttgaa aacaaatcgg gttggccttg atgtgtcaca tttttatatt cactgaaaaa
    17581 ccggtcagcc aaagtcctaa attccaaatc agcaaaaatt gaagataatt tttcgagttt
    17641 aggctcggtt aattccaaat cggaaagcct tgcagaaaca ggaacatcgg ttcttatggt
    17701 tacaagttgt tttgcaagca aaatctgatc gcgaaactgc agcaggtttt ctccaacttt
    17761 accgtaaatt tccgcggcat gttgaatcag gttttcgacc gttccatatt tctcgaggag
    17821 tttttttgca gttttttctc caatgccagg tgctccggga atattatcgc tgctatcgcc
    17881 ccaaagggca agaatgtccc ttacctgatc gggatgctgt accgaaaaac gagattttac
    17941 ttcggcaact ccaataattt cgacctctga ccccgacctg cctggtttgt aaatgagaat
    18001 attttcttca acaagctgag tataatcttt atcgggagtc atcatataca ctttgcaacc
    18061 ctggccggct aatttctttg caagggttcc tatggtatcg tcggcttcga agttatcaat
    18121 ttctattacc ggaatattta acgcttcgat aatttgtttg atgaaaggta ccgacatttt
    18181 tattacttcg ggtgtagctt ctcggttggc tttgtaagcc gggtaaattt ctttccggaa
    18241 actgggtgcg ggtggatcga acactaccgc gatgtgggta ggtttttctt ttttgagtat
    18301 ctcgagcagg gtggtggtaa atccaaaaac agcagaagta ttcatgccct tggaatttat
    18361 ccttgggttt ttgaaaaatg cgtaataact cctgaaaatt agtgcatatg cgtctagcag
    18421 gaaaactttt gaagacatgg ccattcgttt tttcaaaagt attaaaaatt tattgaaacc
    18481 ataatgaatt tgcatgtcta accctattgg gcattaatca tgttgacaat tctcaggttt
    18541 aatgattggt tgtaaataaa gaaataattt gcaggttttc agtaaataat tacctttgca
    18601 cccaagcccg gatggcggaa ttggtagacg cgctggtctc aaacaccagt ggtagcaata
    18661 ctgtgccggt tcgaccccgg ctccgggtac tgtaacacag atacgtgctt ttaatgaaca
    18721 ggctatgaca aaccatccga atcccgaaaa acaacgccag ttcgaccaac actatcttga
    18781 aatggcacag atatgggcta aaaacagtta ttgcaaacga aaacaagtag gtgccctgat
    18841 tgtgaaagac aggatgataa tttcggatgg atacaatgga actccttcgg gttttgaaaa
    18901 tgactgtgaa gacgaaaatt ttgttacaaa accgtatgta ttgcatgccg aagccaatgc
    18961 tattaccaaa gtagccaggt cgggaaacag ttccgaaggc gccacactgt atatcaccac
    19021 ttcgccttgt attgaatgtg caaaattaat aatacaatgt ggcattcaac gggtagttta
    19081 tcttgatgaa tatcataacc gcgatggcat agagctactg aaaaaggcaa agattgaagt
    19141 gataaaattt aaggtatgat gttgtcgccc cgaaacaggt tatttctacc tttgattatt
    19201 gccatttcgg taatcgccgg aatgttgctt tcgttggcgt tgttccggaa tcagaccaag
    19261 gaggttttgg gttttaaact gccgaagaaa gataaactaa gcacaatcat cgattatgtg
    19321 aaaagcgagt atgtcgatga agtcaatgtt gattcattgg ttgaactggc aattcccaat
    19381 ttcctccggc agcttgatcc ccattctgtg tatatgactg ctgatgaagc aaaagcagtt
    19441 aatgaacccc ttgagggaaa tttcgaaggt ataggcattc aattcagtgt aaacaacgat
    19501 actattctgg ttgtggatat catacacggt ggcccttccg aaaaactggg acttaaggct
    19561 ggtgacagaa ttattactat caatgacagt gttgtagccg gcgtaggaat aaaaagcgat
    19621 aatgttgtaa agctgctcaa aggccctcgg ggaacaacgg taaaagtggg tattttgcgc
    19681 aaaggacgtg ataaactgct tcattttaat ataatccgcg ataaaatacc aattctgagt
    19741 attgacgcag cttatatgat tgatgataaa acagggtaca taaaaatcag cagtttttcg
    19801 aaaaacacac acacagaatt tgtagaatcg gcatttaaac tgcatcaggc aggaatgaaa
    19861 aaccttattc tcgacctgag gggcaactca ggtggttatt tagacgctgc taccaatatt
    19921 gtggatgagt ttttaaaagg cggtaaaatg atagtgtaca ccgaaggtcg ttcaaggcct
    19981 agaaattctg tgtatgctac cgataagagg gcggtttgtg tggatgttaa tctcgccgta
    20041 ttaatcgatg aattctctgc ctcggcaagc gaaattgttg caggagcgat acaagataac
    20101 gatcggggat ggataattgg aaggcgctca tttggcaaag gcctcgtaca ggaaccaaca
    20161 atttttaaag acgggtcaat gttgcgcttg actaccgccc ggtattatac cccatcaggc
    20221 agatgcatac agaaaaacta ttccggaggc tatgagagct attatgatga attgatggaa
    20281 agatatcttc acggtgagtt tcaggttgcc gacagtattg atcagaacga ttcactccaa
    20341 tataaaactt ccggagggcg gctggtatat ggtggaggag gggtaatgcc agatattttt
    20401 atacctatcg acagtgtgct tttcaaccca accctgcgtt tgctcagcga ttttggactg
    20461 gcctattcat ttgccttgca atataccgat aaaaacaggg aattgttttc aggtatttca
    20521 tcagtcgatt cacttatcat ggtgctcgac agtagatata ccatgtcgga gtttgggact
    20581 tacattcgca aaaacaagca ttatatttcg tatttcaact ggaataagta tatgccatac
    20641 attaaaagtc aggtttatgg gtatattgta cgcaatgtat tcgacgacaa tgctttctac
    20701 gggtatatca ataaaattga caatacttat ttacaggcag tgaaaactct ggaaagcaat
    20761 gaaagtgtta ccggaacaca aaactcacca tcacataaat agccagggtg agtagtgagt
    20821 aaattaaaaa ccagcgaaca gcagtataat ctttaaggtg gatatgcatt ttatagcgca
    20881 ggtaaagaat cagcaatatt ttttcgagat aatacgccac aattgttgca tatgcaacac
    20941 cgagcattcc gatagttctg atgaataaca cgctgaaaaa aacatttaca acaatctcaa
    21001 caaatgaagc aaacatgata atacctgatt tctgccggcc aatcaatatt gtttgtggaa
    21061 aaaccattcg gctaattatt atcagaatgt aaatattaaa tacttcggca ctttcggtga
    21121 aactctgatt gaaaacaatg ggatacaacc acttgctaag caatagaaaa catatactca
    21181 ccgggaaaag aaaatgcatt aacctaaggc tgcggttttt aagtgagaga aggctatcgc
    21241 tcagctggta attattgctg aattcaggga taaatgcatt gctgaaagca tttgcaagta
    21301 gtattacaaa tggcaattct ctggcaccat atcggaatac tgcaaatgta gcttcattat
    21361 agtagttcga aacaagaaac ccatcgatat attgggcgct tccactgagt aaaagactga
    21421 gtaccagcgg actgccaata tacaggtgtt tttttagata gtctttcgat aatctgagcc
    21481 tggagtattt gtaaacgagg accattaacc agataaagcg gataatattt accagtacca
    21541 acccatataa tccgtatccc agatcaaatc caagcaatac tggtgttgta acacagaaga
    21601 attgcagact aagactaatc agcccgtatt taataatcca acctgattta tttttgacaa
    21661 gataaatgta ttctatcagg ttcactggcc cggaaagtac cacatacatg agtaaaattt
    21721 tcagataggg tatttgccct ccggtgagat taagtaattc ggccagaaac gaacgagaaa
    21781 aaaagataaa aagcgcagcc agaatactga ggattgttaa aatcaaggca gtgttgaaaa
    21841 tatctgaaga ttttgatccg ttttcacccc cggggttttt ataaaaactc aaaaatgact
    21901 gaataatacc cgaaatccag aaaaaactta cccctcctgc gattaatagg aaggtttcgt
    21961 aaataccgat ttcgccaacg ccaagtttgg tttttgtaaa tacaatgctg ataagcagca
    22021 gcatcccaaa ccgtaatagc tggaatgcct gcagtccggt gatgttgttt atgtattttt
    22081 tcaggttcaa aacaggctgt taaaaattct cttgcttatt ttttgaaatt ttcgagatac
    22141 cctgccatta ttttttgaat atatttccca ataatatcaa attccaggtt cacaatggtt
    22201 cctgctttta tgttatgaaa gttggttatt tgccatgtat atggaataat cgcaaccgaa
    22261 aaccggtcat ttttagaatt cacgacagta aggcttacgc cattcaccga aaccgatcct
    22321 ttttcgacag tgatattacc tttggaacta tcatatcgga acgtataata ccagcttcca
    22381 tcggcttcgg caacttctat gcattctgca gtctggtcaa catggccctg taccatatgc
    22441 ccgtcgagca acctgtccat ggtcatgctt cgttccacat taacttcatc tccaaccgaa
    22501 agcaaaccca gatttgactt atcgatggtt tcctgaattg cagtaacttt atacacttta
    22561 tcacttttat tcacttcaac cacagttaaa caaaccccgt tatggctgac actttgatca
    22621 aggtgcaggt cccctgcgaa tgaacaagtt aaatagaaat gataattgct ctgttcctgt
    22681 tcaattttga cgatttttgc agtttcttca acaattcctg aaaacataat gtgttgtttt
    22741 ttagtgaata atctgaacac aaaaataaag taatatttat tgaaccaaga tgtttttatc
    22801 agtttggtag aaaaaggatt tctgggatag gtaaatcata atgatatgat taacattaat
    22861 acacccgaat gtttgctaaa ccagtatttt aagtttctta ttcattaacc gcatgcaaac
    22921 ttgcaaaatc ttgttatttt tgaaaaaaat tcctgcatta tcgcaatgta attaacttaa
    22981 taataatcgt gctgcactat gttgaccaat aaatctgtat taattaccgg agggacgggt
    23041 tctttcggaa aaaaatttgt agagaccatt cttaacaggt accctgaggt taaacgcctg
    23101 gttatatact ccagagacga actgaagcag tttgaaatgg ctcagatgta cccatctgct
    23161 aaatatccgc aggttcgttt ttttattgga gatgttcgcg attatccccg gtttaaaaga
    23221 gcctgtcagg ggattgatgt aattattcat gcagctgcgc ttaagcaagt tcccgccgca
    23281 gaatacaacc ccgatgaatt tattaaaaca aatataaatg gggctcaaaa tgtaattgat
    23341 tcagcgcttg gaagtcaggt aaaagtggtt gttgctcttt cgacggacaa agctgcagct
    23401 ccaataaatc tttacggagc aaccaaattg gtttctgaca aactttttat agctgccaat
    23461 aatattaaag ggagcagaga tttgaggttc tctgtggtgc gctatggtaa tgttatgggc
    23521 tcaaggggtt cggtaatacc cttttttatg gacaaggcga ggcggggaga agttatacca
    23581 attaccgaca aggagatgac gcgttttaat atttcgctcg aagaaggtgt tgaaatggtt
    23641 ctttgggcta tcgaaaatgc aatcggaggg gaaatattcg ttccgaaaat tccttcgtat
    23701 aaaatcgaag ccgttgcaaa agccattgct cccaacgcga agctcgaata tgttggaatt
    23761 cgtccgggag agaaaataca cgaagaaatg attaccacca gcgattcgtt ctatacaatt
    23821 gatttaggaa aatattatgc aatattgtcg gcaggagcac ataaagaaaa atatcttgat
    23881 tattataaag ccaaagaagt tcccaggggc tttcagtata attccggtga aaataccgaa
    23941 tggattgatg aaaaccaatt acgtgagctt attgtaaagt acgtagatcc gaattttaaa
    24001 ccagtgttat gaagccgata ccttatggtc ggcaaagtat aaccgacgat gacatcaaag
    24061 cggttgttga aacgctgaaa tcagactttc ttacacaggg tccacgcatt gccgcattcg
    24121 agagtgcttt tgctgaatat acgggaagca aatatgctgt tgcagtagcc aatggtactg
    24181 cggcattaca cctgtgtact ttggcgcttg gagttaaaaa aggtgataaa gtaattacta
    24241 cccccataac ctttgcagct tcagcaaatt gtgtgcggta ttgtggagga gaggtagtct
    24301 ttgcggacat taaccccgaa acatacttac ttgatattga atcggttcgc catatattag
    24361 aaaataataa ttctggtacc atcaagggaa taattccggt tgattttgcc ggacgggcag
    24421 tggatatcgt agcttttcga aaacttgccg atgaattcgg actttggatt attgaagacg
    24481 cctgtcatgc cccgggaggc tattttaact atggcaaaaa tagccaagtt tcttgtggca
    24541 gtggacaata ttgcgacctt gccatctttt cttttcaccc ggtaaagcac attgctgccg
    24601 gagaaggagg gatgattacc acaaataacg aagagcttta caacaaattg atactactta
    24661 gaactcacgg aataaccaaa ttgccacagc tactcaacga aaaccacggt ggatggtatt
    24721 acgaaatgca ggagttggga tttaattacc gcctaaccga ttttcaggca gcgctggggc
    24781 actcccaatt aaagagagcc gatgaaggaa taaaacgccg aaaggagata gcgaaaaatt
    24841 actttgaagc cttgcaaaac ttgccgttta tcgatggaca gtcgggtgtg gttgagggac
    24901 atgcgtatca cctgtatatt attgaagtag aaaggcgaaa agaaatgtac gattatttga
    24961 agtcaaacaa catatttgct caggtgcatt atattccctt gcacctgatg ccttattacc
    25021 gaaaacttgg atggaagccg ggtgattttc caaacgcaga gaattactat cagaaatgtt
    25081 taagtttgcc aatgtatccc actcttagcg atgttgagca agattatgtg attgatacaa
    25141 ttactaaact cgagaaatga ttgaatcgaa aataggcctg ggtacggtac agttcggact
    25201 gaactatggt atcagcaaca ccgtgggtaa aaccgattca tcagaggttg ggagaattct
    25261 ggaagtcgca aggatgaatg gtatcaatac cattgacact gctagcggct atggtgaaag
    25321 tgaaaaaata ctcggtatat ccgatgtaaa agaatttcat attgtatcga aatataaacc
    25381 tccggttgag ggattttcga ttaaaaacca gctgaacaac agtctgaatc aattgagaat
    25441 caaagcacta tatggttatc tggcccatag gccgatggat ctgcttcagc acagggaaca
    25501 atgggaagaa atgttacttt ttagagaaat gaagttggtt gataaaattg gcttttcatg
    25561 taacgaaact gctgaattgg agcttttact gggaaaaaac ttcatccctg atattatcca
    25621 ggttccctat aattatttcg ataaacgctt tgaaccttat ttcctgcaat tgaaatcgaa
    25681 gcatgttgaa atacatacgc gatcatcatt tcttcagggt ttgttttttg ttccggccga
    25741 taagttgcat ccgtatttcg aaagggttaa accgcttatt ggagcacttc aatgcaaata
    25801 cggcaacaat ttgtcaagga ttttattagg tcattgcctg aaaaacccgt caatcgataa
    25861 ggtaattatt ggagtgaaca atgtaactca attgaatgaa aaccttcgtg ccggatacga
    25921 gggagatatt ttgccggatt taaatgaatc aattcctgat aatatagtaa tgccatcgca
    25981 atggaaactg taaacaaata tgcgcaaatt tttgacctga gtgaaaaaac agcaattgta
    26041 accggtgggt acgggcattt aggctctggt attgtcagag ctttgctggc ttttaatgca
    26101 gatgttgttg tagcgggtcg ttcaatcgaa aaatatgaag ctgtattcag cgaagcggaa
    26161 aggaaaaatc tttcgtttga gcaaatcgac attaccaatt cccaaagcat tgccaattgc
    26221 tttgaggttg ttgtggaaaa atacggaact atcgacatcc tgattaataa tgcacataca
    26281 gccaaaggaa actctcctga aaatatgaac gacgaagatt gggcctatac aatggacggg
    26341 gttgttggct ctgttcataa agctatccgc gaaataatgc cggtaatgaa aaatcagggt
    26401 tttggaaaaa tagtcaatat tgcgtcaatg tacgggttgg ttagccctga tttcaggtta
    26461 tacgaggatg ataattgcga aatgtaccga aatccaccac actatggtgc tgccaaggca
    26521 gctatgatac aacttacaaa gtactatgct gcactattgg caaaatataa tattcaggta
    26581 aatgcagtat caccgggtcc attccccaaa gctaaaatac agactgataa tccgctattt
    26641 gtagaaagac ttaaaaagaa aaatccaatg aacaaaattg gtgaacctgt tgatctggcc
    26701 ggggtaatcg tgttgttaag ctcccgtgga tcttcgttta taacaggtca gactttacaa
    26761 gttgatgggg ggtggactat atggtaaaag ataacattac aattatcctt caggcgcgga
    26821 tgagtagcga aaggcttccc ggaaaagtaa tgaaaaaaat ttccggaatc ccgatgattg
    26881 gaatacaaat agagagactg cgaaaatcag gaatacccat tgttgtagct tcttcggaat
    26941 tgcctgaaaa cgatgttttg gaagattatc taacgtcaat tgaaattccg attttccggg
    27001 gaagcgaact taatgtactc gaccgttttt accacgcagc aaaaaagtat catgcggaat
    27061 atgttattcg cataaccggt gataaccctt taatcgatgg cacatttctt gggaatacaa
    27121 tttcaggaat tcagaacttt gggaaacgat cttatttttc aatcggacga agcaatactt
    27181 tccccctagg cttttctttt gaattgtttt cgtttgaatt acttgaagaa gcgtacaata
    27241 atgctcaaag cccaaaggaa aaagagcatg taacaccata tattcatcag aatgttccgg
    27301 ggaatattat aataaaaact ttatacaggg aaccgacgcg aaacgagtac agattgtcgg
    27361 ttgatactaa cgaagactta atattggtaa aaacgctgat agagaattac gactgtcgca
    27421 ataaacctat ggatgaaatc attgatattc ttgataagaa tccggaactg gtggctatca
    27481 acaatcgtat cacgcaaaaa aaatggaatg aataatattc cgatttctaa atcaggaaat
    27541 gttgaaaacc gtcaagaatt ctgataacaa ttttttatag tcggaacagt gcaatgcaat
    27601 gttatttgac aagcctgttg ttttttcaac aatttttatt attattgaaa agttaaaagt
    27661 gtactttttt tatgcttaaa acctattttt tcattccggc taatgttcag aaatttatag
    27721 aaaaagctgc cggcattcct tcagattaca ttgtttttga ccttgaagac tcggttgcat
    27781 tcaatgaaat tgatgaagca ctagggaatt taaaatgtat tgatatacgg aaaaattatt
    27841 ttgtccgccc tgcgttgttt gcaaaaaaca agctcgatgt tgacaaagat atgctacatt
    27901 ctttaattga tattgggttt actaattata tcattcctaa attttcggac gttctgcaac
    27961 ttgcgaaact aaaagaaata tttaccgtat cgtcgcaata cgattatcat acgtttaaat
    28021 ttgtaattct ggttgaacat cctgccggat tactttttct gaaagaagct gttcaaagca
    28081 agcaattgaa tatttctgca cttgccctcg gtaactacga ctattgcaat gcaatgggta
    28141 tgaagcataa tcttgagaac ttatattatg caaggcaaat tattttaaat actgcgaggg
    28201 catacgattt aagcgcaata gatattgtta ctctcgatta caaagatagg gaagcctttt
    28261 gcagtgaagt attgaacggt ttctcgatgg gtttcgatag caaattttta atacacccaa
    28321 tgcagcttga acagatacat tccattaaat actactccga tgaagaagtg gctgaagcca
    28381 gagaattata tccaaaaatt ctggaaatga tgcaaaacaa aacttccatt gttaaattaa
    28441 atggtaaaat tttcgaaaaa ccccatatta accgaattat taatataatt aactggaaca
    28501 atcgctatgg aagcaaatag aatgggccat tacttcgagg aattcgaagc agggcaggta
    28561 tattatcatt gtcagtcaaa aacaatattt gaaagcgaca acaacatttt tagtttatta
    28621 acaatgaatc accatccggt tcatactaat atcgattact gtagcaggca gcaacacggt
    28681 caggtgctgg tgccggggac tttggttttc tgcctggcag ttcatttgac tgtaatggat
    28741 ataagcggac aggcaattgc aaatcttgcc tatgaaaata tcgatcatct ggcaccaaca
    28801 tttataaacg atactattta tagccgtacg gtaatactcg aaacaaagga atcgcaatcg
    28861 aaacccgaca gaggaatcat atacgttgaa accattggtt acaatcaaaa caatgttgat
    28921 gtaataaaat tcagaagaaa agtccttatt aagaaaaaac cccaataata tgaatgaaaa
    28981 aaaatttata atgcgcagcc ttatgtttgt gccagggcac aacgaaaagc tgcagataag
    29041 tgccgccaaa tcggacgccg atgtaattct tcttgatctg gaagactcgg ttttacccga
    29101 atcgaacaaa caactggctc gtgataccat tgtaaaaaat gttagtagcg gaatgtttaa
    29161 agactatgcc gtatttatac gcattaacga aagacagagc ggatttctgc ttgacgatgt
    29221 aatgcaaacc actattccag gagtggatgg gtatttattc tcgaaaacct ataacgcaca
    29281 ggacattgtt ttcttcgata aactgctcga agccattgaa tataagaaag gaatgccggt
    29341 tggaacattt aaaataattc ccattcttga aacatcttcc tcaattatta aagccgatga
    29401 aattgcaacg gcatcaagtc gcaatgttgc cataggtttt ggttccgaag atttcgtaag
    29461 cgatttacaa ggaatcaggg acttcgacaa agccgttagc ttgttctcac ctcgtgcatg
    29521 ggttgccatg gttgcaagaa cttataatct tattcctatt gatgctgcgt atattaaaat
    29581 tcacgacgag gaaggtcttg aaaaacattg tcagatgggt agaactttag ggtatgcagg
    29641 aatgtggaca ttgcatccca agcaaaatgc tgttcccaac cgacattatt ccccttcgga
    29701 agaagaaatt aaagaagctt acgaaattct ccgccttgat gaagaagccc gaagtatggg
    29761 taaaggcgtt gcaattataa acggcagttt cattggaccg cctcttgtgg ttaaagcaaa
    29821 acatattatt gaaagggtta aattaattca atcgaaacaa aaatgaaact gattattatt
    29881 ggaggttatg gcaacggaac tgtaattgcg cagattgccg aagaaataaa caaagtgaaa
    29941 cacacttggg atatattggg attcctcaac gactttgaag aaggatatat caatgagtac
    30001 ccaattctag gaaaagttga gcacgacgtg gctcaaaaat atcttgccga cccggatgtg
    30061 tatttctttt acgcattgat ttctataaaa ttaaacttca attttttgta taagcttcat
    30121 gaccttaata ttccgctgga acgatttgca aatttaatac acccaaatgc cgttgtttcg
    30181 aaagctgcaa ccatagggca cggggtgagt attcaggcat tttcgattgt cggacccaat
    30241 gcgacaatgg gcaattttat acaagtttac gggcatgcta ccgtagggca taattgcact
    30301 gttgatgatt atgcctatgt aacctctaat tcaattgttg gagcacacgt tcatttgaaa
    30361 cagggcgcat ttcttggagt aaattctacc accctcgaca gggttactat tggtgaatgg
    30421 tcaattgtgg ggcaacactc aaatgttatt agagacgttc ccgattacac caaagtggtt
    30481 ggaaacccgg ccagaattat tggtgaagtc aaatgaaaat tctattcaga acagacggaa
    30541 actctcagat agggttcgga catctgattc gctgtgtttc gatagtaaac gaaattagaa
    30601 aacaatcgga attacattct cctgaaattg agtttctagg gtctttttcc gatgcagcac
    30661 aagaagtttt gcttaatgaa aagattctgt ttcaacttgc agaaacgcat gaagaattca
    30721 acttgctttc ggaaaacatt agcatttttc gtccagatgt actttttatc gacagggtat
    30781 ataattattc ggaagaagaa atcaaagcac taaaaaaaaa ctgccgtata ataatgatgc
    30841 actatataag tccgggaatt cgtacctgcg accactatat actaccatcg gaacacactc
    30901 ctcagaacct tattgatgag tcgggtatga tgacagggaa aggacaggta catatgggtg
    30961 ccgactacat cgttcttaac cataagttat ccgggcttcg caggaataca tacacaccgg
    31021 cacaacacat ccaaatagct ataaccacag gtggcagcga ccccaaacag gtgttgctga
    31081 aaatactccg gattctaatg gtttattcat gggaaaatgt tcgttttaaa gttctctatg
    31141 gactgtcaat agcatttggt gaggaactcg aaaaattgaa aaaagtttta cctgcatatt
    31201 tcactgttat accatacaat gagaaagaac taatcgatgc cgatttggcc atatgtacct
    31261 ttggaattac gacctacgaa ttactgtatc tgggtattcc ggtaatttcg gtatctcatg
    31321 ccgaaagcaa cgccctgggc agtaaaactc tggcccaaaa acatcatgac attattgctg
    31381 atttgggatt ggcagaaaat cttacttctg atgctttgat tacatgtatt aaaagcttga
    31441 ttcaggatgc cgataaacga atttatatgg ctgaatcagg aaaattgctt gttgatggcc
    31501 gtggtgtttc gcgcgttgct gaaataatta taaatagcat gaatcaatga taaagcaaca
    31561 aaaaaccttc ataattgctg aactatcggc aaatcacaat caagatttca atcttgccgt
    31621 aaaaaccatc gaagctatgg ctgaagcagg tgccgatgcg gtgaaaatac aaacatttaa
    31681 acccggaagt ctgacacttg accttgatac agggtatttt gcaccgagaa cagagggttt
    31741 gtggaaagga tataccccat ggaaactatt tcaggaggca gctatgccat
//
LOCUS       DAOOQP010000020        31703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1640_length_31703_cov_6.373610, whole genome shotgun sequence.
ACCESSION   DAOOQP010000020 DAOOQP010000000
VERSION     DAOOQP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1640_length_31703_cov_6.373610"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLK75_03735"
     CDS             complement(<1..627)
                     /locus_tag="PLK75_03735"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOY38386.1"
                     /translation="MKKYSIDLANYERFPSKEVKIGHVPLGAMHPIRLQSMTNTQTSD
                     TQNTVDQCIRLFEAGADYVRITTPTQADAENLTQIKKLLQEKGFTNPLIADVHFNPVI
                     AETAARIVEKVRINPGNYYDRNHHRLNSQVDEPRFELEKIHSRIKPLISICKEYGTAI
                     RIGSNHGSLSERILKTYGNTVEGMVEAAIEYIKIFVAEGFYQLVISMKA"
     gene            complement(637..1065)
                     /locus_tag="PLK75_03740"
     CDS             complement(637..1065)
                     /locus_tag="PLK75_03740"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOY38387.1"
                     /translation="MRGIIITGFFLFFLLPVFSQTDCILVTINSIRSTKGVVRYALYN
                     EKNEYNSHTDIFKEGLVEVHGSTVRILIKGVPDGIYAISVLHDENNNGDLDMSAVGIP
                     KEGFGFSNDAKVTFGPPKFESAKFVKKEETAIRVKMRYML"
     gene            1150..2715
                     /locus_tag="PLK75_03745"
     CDS             1150..2715
                     /locus_tag="PLK75_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY38388.1"
                     /translation="MSKEKVFIAGAAGSMGFETFKSVWNKCDTNGDRRFDIVLLLFDS
                     RKNRKLLAPYLFECGIGFSRSKIIEKNGLKVIWGDGRKYEDIQKAVSGVDFVLNCMAI
                     ISPRAEKEPENTKNTNLKAVEYIIKAIKEQPNGTEHIRFVNVGSIAEYGDRMGRIHAG
                     RVGDPVVVSPFDIYAITKVKAERLVIESGLKHWVQLRQTFIMIPNVFSLEDPIMFHQP
                     IGCFMENITARAAGKGMANCLEIPHNSDFWRKVYNMGGGPECRTNYLDFLNDMFGLMG
                     LRYQKIFERKWFALRNFHMQYYADSNILNEYLNHWGDTLDDFKKSVSHNLPWYLKTVV
                     KLNKSVPAFRNMVEKITRRRMKNMAMRRTGNLRWITENNDLRIAAFYGSRDAYDAIPD
                     WDAPLPEGLAGTPEPECIQLNHGYDQTKESLEVSDLQSAANFRGGKLISEEWDQDMYC
                     QMVWECEFGHRFEASAYAVLKAGHWCTVCEAPPWNYDEIARRNPFFAQVWHESHSPDE
                     DHFYPELCYDDIL"
     gene            2759..3490
                     /locus_tag="PLK75_03750"
     CDS             2759..3490
                     /locus_tag="PLK75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOY38389.1"
                     /translation="MTVLAVVVFVSLQFVEAGYGKFKNKKWGFSIKNKIGWFLMETPV
                     FIAMFLLWLFSERGFEITPLIIYVIFMSHYFHRAFIFPFRLKGKNTMPVAIVLMGVSF
                     NMLNAFMQAGWLFYFAPSDMYSVSWLYSPQFIAGFIIFVAGMFVNISSDSIIRNLRKE
                     GDNNYYLPKGGMFNYVVSANYFGEFLEWVGFALLSWSWAAVVFAVWTFANLAPRAKST
                     YRKYSLLFGDEFHRKKLKYIIPFIY"
     gene            3516..4427
                     /gene="gldA"
                     /locus_tag="PLK75_03755"
     CDS             3516..4427
                     /gene="gldA"
                     /locus_tag="PLK75_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOY38390.1"
                     /translation="MSIEVKNITKLFKKQKALDDVSFSIHSGEIVGFLGPNGAGKSTM
                     MKILTGFIPPTSGDAYIHGVHVLEDSIHIKRLIGYLPENNPLYTDMYIREYLNFVAGI
                     YKIGSNRSKRINEVIELTGLEPEISKKIGALSKGFRQRVGLAQAIIHDPQVLILDEPT
                     SGLDPNQIIEIRNLISELGKKKTVMLSTHILQEVQAICQKIIIINKGKIVANEDAAIL
                     QEESAGTKKSIHIVFETPVSKKSLTEINGVEKVASLSECEFVLQSCSKKDIRPEIMKF
                     AVKNNLNILSMAEQSKSLEQVFQDLTK"
     gene            complement(4487..6547)
                     /gene="htpG"
                     /locus_tag="PLK75_03760"
     CDS             complement(4487..6547)
                     /gene="htpG"
                     /locus_tag="PLK75_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOY38391.1"
                     /translation="MASKKGKIGVSSENIFPIIKKFLYSEHDIFLRELVSNAVDATQK
                     LKILAAAGEVTHELSDARVYIEVDEKNKTILIRDHGIGMTEEEVKKYINQIAFSSAGD
                     FLEKYKNDANAIIGHFGLGFYSAFMVSEKVEIITRSFRENAQAVLWSCTGDPEYTLKE
                     TEKDSIGTDVILHIDKDSEEFLEKNRISSLLNKYCKFLPVEIVFGKKTEWKDGKETET
                     DEWNVINNTQPVWTKKPADLKDEDYAGFYRELYPYSFEEPLFNIHLNVDYPFNLTGVL
                     YFPKIKSNIDIQKNKIQLYCNQVFVTDSVEGIVPEFLTLLHGVIDSPDIPLNVSRSYL
                     QNDANVKKISGHISKKVSDRLAEIFKNDRADFEKKWDDLKLFIIYGMITDEKFYERAE
                     KFSLLKNTDGKYFTFEEYKQLIELEQTDKNKTVVYLYATDSETQFSYIQAAKNKGYDV
                     LLFDGQLDMHFINHLETKFKNSHFVRVDAETADNLVKKEKTEESKLSELEKQRIRLMF
                     EGVLPENDKYIVGVENMNESDMPVLLTRDEFMRRMKDMSHFGSGTSFYRDMPDSFRLV
                     VNSNHSIIVELSREAESELGETLIEISSSVEPLKKLLEELTEKHKGKKDADIPAEEKE
                     QKIQLQEQVDAMNENRSNLLRNFGKSRQIISQLFDLALLSNNMLKGEALTRFVSRSLT
                     LMEK"
     gene            complement(6626..7147)
                     /locus_tag="PLK75_03765"
     CDS             complement(6626..7147)
                     /locus_tag="PLK75_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY38392.1"
                     /translation="MKSTKHIDFYFVDDDLLYLSFFRKQFKPDLSFSLFTFSSGEAFL
                     NKFKRDQGNKNLKIVILDYVLNSEDINARTGLELLPIIKEIDKFAEIVILSGFDNMDV
                     KATSNSKYPFDYIKKNDHTFVRLYSTINTIMSTYNLKKKRQDMKIAWYVFGVIGTLLI
                     SCLILLFIFSSQL"
     gene            complement(7271..8167)
                     /locus_tag="PLK75_03770"
     CDS             complement(7271..8167)
                     /locus_tag="PLK75_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38393.1"
                     /translation="MPRKIHTAVLLCSLFIVTNNVFSQKNDDLQYAVDSIASWLNQIS
                     VSNDDSEKIRINNYVISYMDMVLHDDASFEMNFDSVKYLKVLDTDSKKLRIYTWNIFY
                     EDGSFTYFGYLQYKKNKELFTYQLLDCSDDTVGNKRSFTSYKEWYGALYYEVVEKKWN
                     NKEQYVLIGWDGADRLINRKIVESLSFSRSGLPVFGEKVFVVGKEKKGKLFYEYSEMA
                     TMILRYNQQNDIIVIDHLAPSNSRFTGHYQYYGPDFSYDALEYNSGKWYFKPDIDPNK
                     AINYEKNKRIDRLQRRGASKNF"
     gene            complement(8223..9413)
                     /locus_tag="PLK75_03775"
     CDS             complement(8223..9413)
                     /locus_tag="PLK75_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOY38394.1"
                     /translation="MASNRELFLKVLGQTSETPYLLEIESAQGVFLKDLSGTEYLDLI
                     SGVSVSNLGHNNPEIVKAVCAQAADYMHLMVYGEYVLGPQVKLAEMLCSVLPEKLNSV
                     YFVNSGSEAVEGAVKLARRYTGRNRVMSFKNAYHGSSAGALSLCGNEDFKNSFRPLVP
                     GIHLAELNSFEELINIDSETACVILEPIQAEAGVMETDTQFMKALRSRCTETGCLLIF
                     DEIQTGFGRTGKMFAMDHYGVVPDIVCFAKALGGGMPLGAFVAEKKVMNSFTSNPVLG
                     HITTFGGHPVSCAAALASLQIITAGQLWINANTSEKIFRQLLSHKLIKTIRGKGLLLA
                     VELQSVEVTMQFVRAALTQGLITDWFIFHPTAFRIAPPLNITRDEIQLACAKILKTLN
                     SINL"
     gene            complement(9416..10129)
                     /locus_tag="PLK75_03780"
     CDS             complement(9416..10129)
                     /locus_tag="PLK75_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY38395.1"
                     /translation="MNCIIVDDDQLSRNLIEAYIERTQTLSLSGSFSNPIEALHFLQS
                     ENNSIDLLFLDILMPEMNGFQILDTLDGKQQIIVISGKKEFALDTFQYGVTDYLLKPI
                     TYERFFKAVDKAYERNREIYPQKNSDNFLVKIGTSFRKVNYNDILFIEADNENTYLVT
                     DENKFKIISNLEELEVFLPEPAFLRVHANYLVNLNKVSSVEDEALTFLINGNTLNIPF
                     ECLAKKTIIGKIELSKVKV"
     gene            complement(10190..10999)
                     /gene="murI"
                     /locus_tag="PLK75_03785"
     CDS             complement(10190..10999)
                     /gene="murI"
                     /locus_tag="PLK75_03785"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOY38396.1"
                     /translation="MRNDEPIGVFDSGIGGITILKSLINAMPFENFIYLGDSGNAPYG
                     EKPVEEIVSLTVKNVEFLFKQKCKIIVIACNTATGAAIHFLRNTYPGYDFVGLEPAVK
                     PACLASKTGNIGVLATEGTFKAQHFRSTSEKYAEYIHIHTQPGYGLVNLVEQDMIHSP
                     EAESLLQKYISPLREKNIDYLVLGCTHFPFFKKTIQQICGDEVAILDSGEAVARRTKD
                     ILMTKGLMKSVNNLGFIRIITTGDVAILQSVAGSYLEIDQANYNFSHFDRL"
     gene            complement(11048..11548)
                     /locus_tag="PLK75_03790"
     CDS             complement(11048..11548)
                     /locus_tag="PLK75_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY38397.1"
                     /translation="MKKLILITLPILLVFTAYAQKSKFGHVNSAAIFEAMPERAEAKS
                     AIEKYAKQLEDQLLAMNSEFEQKYNAYLEEAETYSPAIKQSKEKELSDLQSRIQNFQM
                     SAQTDLQNKEQELLEPIYNKIKDAIKQVGQENSFMYIYDISTLLYYSDESIDVTEMVK
                     TKLQKK"
     gene            complement(11603..12115)
                     /locus_tag="PLK75_03795"
     CDS             complement(11603..12115)
                     /locus_tag="PLK75_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY38398.1"
                     /translation="MKKTILLLLVSVFSMYAWAQKYAYVDTEYILNNIPLYESAQEQL
                     NELSEQWKAEVDAMQTQVDKMYKDFQAEKVLLTDELKEKREQEIVNKEREMRDLQKKY
                     FGRDGMLFKKRQELIKPLQDDIYNAIKEIAVEGSYAVIFDTANSLNMLYTDPKYDKSD
                     EVLKKMGYKN"
     gene            complement(12186..14720)
                     /locus_tag="PLK75_03800"
     CDS             complement(12186..14720)
                     /locus_tag="PLK75_03800"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOY38399.1"
                     /translation="MKKIAFLLFIVYLVTSVTAQEDISSRIKKLDYSDPVEYEIGGIQ
                     ISGIKYLDPDVLVHLTGLQVGDVIMIPGDEITAAINKLWEQGLFSEIKISLEKVVNKK
                     AFLDIYLVERPRLSKFSFTGIRKSEADDIKEKIKLVRGNQITENTLINTKNIITDYFV
                     EKGFLNVDVNIESSLDSSMVNHVILKININKNERIKINEIHFSGAIAIGKEKKWYNFY
                     RSPEKITDSRLHRTMKETKEKRWYGFFKASKMINEKYEEDKAKIIEKYNELGFRDARI
                     VSDSIVKFDNNTIDVYISIEEGPKYYYRNITWVGNTKYSADFLSQVLGIRKGDVYNQK
                     LLEEKLLYDPQSVFSLYQDNGYLFSNINPVEVYAENDSLDIQMQIYEGKQARINKVAI
                     SGNTRTNDHVVMREIRTSPGSLYRRSEVQRTIREFAQLGYFDPEKLNVDFQPDPAKGT
                     VDLEYLVEEKPSDQIELSGGYGAGMIVGTLGLSFNNFSLRNVFNPAAYRPLPSGDGQR
                     LSIRAQASGLYYQNYSFSFVEPWLGGKKPNSLTTMIYHSITTNGSSDAEERTSFKVTG
                     AGLGLGKRLAWPDDYFTNYTELSYRYYLVDNYPGFLYKEGHSNNVSIKTVVGRSSSGP
                     NPIFPTMGSEMSVSIELTPPYSLFANADYASMSANDKYKWIEYHKWKFSGAWFTTLVG
                     SKSGDSRALVLLTKFEAGILGYYNSDLGPSPFEGFQLGGDGLYGYNLYGRETIGLRGY
                     LNNSLTPGDGGNVYDKFTVELRYPLSLNPSATLFALAFVEAGNAWYDIKKFNPFDVKR
                     SAGVGIRIYMPMIGLLGVDWGYGFDEINGSTGANGSQFHFVIGQTF"
     gene            complement(14730..15473)
                     /locus_tag="PLK75_03805"
     CDS             complement(14730..15473)
                     /locus_tag="PLK75_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOY38400.1"
                     /translation="MSLLEKLDKTRIPVHIAIIMDGNGRWAKEKGQPRMFGHKAGVQA
                     VKRAIIGAREAGVQYLTLYAFSTENWNRPQTEVLGLMDLLVNSIESEIEELHKNGVCL
                     RILGDMSRLSRKVYQKLQFACEKTKNNSNLQLLVALSYSGRWEIAEAARRIATDACNH
                     SITPDEINESLFARYLTSADIPDPELLIRTSGEMRISNFLLYQLAYSEFYFPPLHWPD
                     FTKETLFEAIYNYQNRERRFGKTSEQIKN"
     gene            complement(15481..16215)
                     /locus_tag="PLK75_03810"
     CDS             complement(15481..16215)
                     /locus_tag="PLK75_03810"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOY38401.1"
                     /translation="MKPVTKLITGCLLILSSLIHGAAFGQRSGGGEIGVMAGGSYYMG
                     DINLAIPFYQPHLNLGGFYKHHLNSRLSVRVGFFASSLSADDQDFNSYYQKLRNSRFS
                     TNMMEISLQTEFNFLPYLMGELRKRNYTPYLQTGLAYYLTPGSAKVTGLSIPVGLGFK
                     VNISHRMVLSAEWGFRRTFTDLLDNTTGEDLYQYNTVDNIPYNATTGIRQIGFAMNKD
                     WYSYASLGLSYCFSWGGFPCPAYDVY"
     gene            complement(16303..17193)
                     /locus_tag="PLK75_03815"
     CDS             complement(16303..17193)
                     /locus_tag="PLK75_03815"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOY38402.1"
                     /translation="MKKIFFLLIFSVFCSAVVNAQLGTSWKKIRHEIYFGAGPTNFLG
                     ELGGANQIGTHFLRDFEIKATRAVFGVGYRYKLAPDWAVKTGLWYGWLSGADSLTLQP
                     DRHDRDLSFRSPVLEWTANIEYSIIKERYGHRYDLRRVKGKKNLPNLYGFTGISAFYF
                     NPKAKYTDGEWYALRPLGTEGQGLVPTREKYGYVAIAIPMGFGMNYMLDRNWGIGFEY
                     GFRYTFSDYVDDVSTTYVDPNLFEDPIARYFSDPTLGTWIGTGPLQQRGDDHYTDCYM
                     FLTINASYKLRPRQPGMPKF"
     gene            complement(17230..18111)
                     /locus_tag="PLK75_03820"
     CDS             complement(17230..18111)
                     /locus_tag="PLK75_03820"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOY38403.1"
                     /translation="MNVAVYGRNFPKKFNGAAIQLFEALHDAGCKIFMNDQFLHFITE
                     NLMYKPVLSGVFHDKLPDNENFDLMLSVGGDGTFLETITCVYNRNIPVLGINTGKLGF
                     LANVSQDQVLGTVKQIVEKKYTLTKRFLLECNTQQPIFEKYSFALNEFTTQKLHTLNM
                     IKIKVVCNGEYINTYWADGIIIATPTGSTAYSMSCGGPIMTPDADCFVITPIAPHNLT
                     LRPLVIPANSEIKLQVEGPVREILVTLDYRSKAVGSNVEFSIKKSDFHINVVTLSQNY
                     YFSTLREKLMWGIDVRN"
     gene            complement(18114..19496)
                     /locus_tag="PLK75_03825"
     CDS             complement(18114..19496)
                     /locus_tag="PLK75_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOY38404.1"
                     /translation="MKTVFLKYFFTCVLICVSLVQYVEAQQWYVVDSIIISGNQKTVP
                     EIILDELEFAKGDTITQELLEASIRHSENKLRLLGLFNFTDIAYSVNGSSVSVFISLV
                     ERWYLWIYPIFELADQNFDTYFFNRQWERLNYGVSFEKHNVLGRNLLLKAKIRFGYKE
                     QYSIYFSNPGSFRRKSFGYWAGGDFFRKKEIVAGFRDYMPVYESSEPNYLYHQFGVQA
                     GVSYRVFYNSVCKAKLAYHNFYRNTWCTPDRLNLSENEFSCTEFSIKFEHDTRNNKYF
                     PTSGFFAGAEITQYGIPFTDTDKWINQTVFNAEANVFFQFSQRFFTGIETMALLHHSK
                     NTSIPLGLYRSLGYETYLRGADNFVLNSEIYLLCKSSFRYAVFRELYKELNLLKLEQF
                     DKIHYELFLTAFADLGYVKNYNIVAGLGAGFELVTYYDRVLSVYYAYNNLTEKFNIFV
                     QFKTPILKQY"
     gene            complement(19493..20158)
                     /locus_tag="PLK75_03830"
     CDS             complement(19493..20158)
                     /locus_tag="PLK75_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOY38405.1"
                     /translation="MIASELISEAIPCLKTSDTGEKALLWMDTYRISHLPVVNNEEFL
                     GLVSDNDVLDHNCLSDPIGFHKLSQIKPFVFDSQHIFEVIEIVDNLRISVVPVLDKNR
                     HFLGVITKKDLVFAFVKMVNINKSGAIIVLEMNIHDYSLSQIAQIVESNNIKILSLFM
                     SASTDTTKMNITLKVDTTDISPVLQTFSRYNYSVVAYFLSEENTQAHLASRFDSFMNY
                     LNM"
     gene            20233..20946
                     /locus_tag="PLK75_03835"
     CDS             20233..20946
                     /locus_tag="PLK75_03835"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOY38406.1"
                     /translation="MTKLSVNINKIATLRNARGGNIPDVCEAVIRCQQFGAEGITVHP
                     RPDERHIRYSDVINIKPLISTEFNIEGYPSEIFMELVLQVKPTQVTLVPDAHDVLTSN
                     AGWDTAKHQMFLSAVIKLLKDNGIRTSIFVDTSEGNITYAAKTGTDRVELYTEPYASE
                     YKTNRESAIARFIEAAKLAQHFGLGVNAGHDLNLENLQYFHKQIPSLLEVSIGHALIS
                     DSLYYGLENTIQMYLRALK"
     gene            complement(20975..22492)
                     /locus_tag="PLK75_03840"
     CDS             complement(20975..22492)
                     /locus_tag="PLK75_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOY38407.1"
                     /translation="MPLVNSILNLINYGRLDEIEYFRRNPIEVQEKVFKQLIEKASDT
                     VFGKKYDFKNISSRSDFFQKVPVHDYEKLKTYIHRIMNGEQNVLWHSEIKWFAKSSGT
                     TSDKSKFIPISRESLEDCHFRSGKDIFAIFTDHFPDTTIFSGKTLTLGGSTTVNTLSE
                     NSYYGDLSAVLIRNLPFWTYFNRVPKPEIALMDEWEEKMKMMVETTWNKNVTNLIGVP
                     SWFLVLLKKIIEYTGSDNICDVWPNLELFVYGGVNFLPYQEVYRSIIPSSDMKYLETY
                     NASEGFFGIQDDLSSDDMLLMLDYGIYYEFLPMEVYGEPADNTVMLEDVECGKNYAML
                     ISTNGGLWRYMIGDTVRFTSKNPYKIKITGRTKHFINAFGEELIVDNAQKALAVACMR
                     TGAQVREFTAAPVYMGQNNKGAHEWLFEFITPPDSIEKFMYILDIELQHCNSDYEAKR
                     YKDITLEFPKYNVLKEGCFFKWLQQKGKLGGQNKVPRLSNNRFYIEELLNTNKNL"
     gene            22592..23395
                     /locus_tag="PLK75_03845"
     CDS             22592..23395
                     /locus_tag="PLK75_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HOY38408.1"
                     /translation="MEKVFSGTLRKLQTRFDNPVRYSIYNELHDTRVNPLIGRSIRIS
                     HNGVINCIHCGAETKQSFHNGYCYSCFITLAETDAGVLHPELDKSHLGISRDMEWSKA
                     NSLIDHYVYLAVTGNLKVGVTRHTQIPTRWIDQGAISAVKLAKTPYRQLAGQIEVELK
                     KYVSDKTNVAAMLQAVTHSIDLVAQKNLLSEHLPEELQQYVTLDNRITQIIYPIHGEV
                     AYKTNLKLANTPQFEAKLIGVKGQYLIFTGGIVFNVRNHSGYHICIELE"
     gene            23397..23849
                     /locus_tag="PLK75_03850"
     CDS             23397..23849
                     /locus_tag="PLK75_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOY38409.1"
                     /translation="MENIKMKEALNAAKLRCSKGEKCISEVVDFLARFDITEKETAMI
                     TAILIAEKFIDESRYARAYVLDKFRFNKWGRIKIRFELQRKNIPAAIIIDSLDSINEK
                     EYSDVIKFLISQKKIKTDNAYEYRQKMLRYLYGKGYEPEIVEKFINCP"
     gene            complement(23856..24905)
                     /gene="queA"
                     /locus_tag="PLK75_03855"
     CDS             complement(23856..24905)
                     /gene="queA"
                     /locus_tag="PLK75_03855"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOY38410.1"
                     /translation="MKLSQFKYVLPEELIALHPCDNRDESRLLVLDRKTGNIEHRMFK
                     DIKDYFEDGDVFVFNNTKVFPARLYGNKEKTGARIEVFLLRELNKDQRLWDVLVDPAR
                     KIRIGNKLYFGPDDSLVAEVIDNTTSRGRTLRFLFDGSYDEFKKSLYELGETPLPKFI
                     KRPVEDDDEERYQTIFAKHEGAVAAPTAGMHFSRELMKRLEIIGVQFAFVTLHVGLGN
                     FRHVDVEDLTKHKMDSEQIIIPDDAVDMVNNAKLNRRHVCAVGTTVLRTLESSVTTNQ
                     MLKPFNGWTNKFIFPPYDVQVPTRLVSNFHLPFTTLLMMVAAFGGFDMVMNAYKIAIK
                     EKYRFGTYGDAMLII"
     gene            complement(24993..25700)
                     /gene="truB"
                     /locus_tag="PLK75_03860"
     CDS             complement(24993..25700)
                     /gene="truB"
                     /locus_tag="PLK75_03860"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOY38411.1"
                     /translation="MQLFDGSNVDFMAGQLILVNKPLAWSSFDVVNKLRQLICKQIHV
                     DKLRVGHAGTLDPMATGLLLICTGRATKEIESLQDFDKEYLATLKFGATTPSFDSETE
                     MDACFEFSHITKDALIICLKNFVGEIEQIPPHFSAVKLQGKRAYKIARKGKNPEIKPR
                     TIKITQISLEHFNLPIVTIRVCCGKGTYIRSLARDIGAAMNSGAYLTGLIRTKSGDFS
                     LEDAYEIDEIEKIIRNM"
     gene            complement(25704..26489)
                     /locus_tag="PLK75_03865"
     CDS             complement(25704..26489)
                     /locus_tag="PLK75_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOY38412.1"
                     /translation="MSWFEALILGLVQGLTEFLPVSSSGHLEIGKAIFNIKGEENIYF
                     SIAVHGATVLSTILVLRKELTRLVSGVFKFKLNEETHYLLKIAVSMIPVLVIGLLFKD
                     YLKTLFDGSNMVFIGLMLLVTALLLTFTYFFKPKQKPISYFDAFIIGVAQAIAVVPGI
                     SRSGATISTGLMLGNKKEDIAKFSFLMVIIPILGENFLEIVSGNFSGSSGIEIMPLIV
                     GSIAAFISGWFACSFMIRIVQRGKLIWFAVYCAVIGLVSILFL"
     gene            complement(26501..26797)
                     /locus_tag="PLK75_03870"
     CDS             complement(26501..26797)
                     /locus_tag="PLK75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOY38413.1"
                     /translation="MALKKETNIPPKQNNADEFALTKQNYILLLIGFIIIVIGFVLMA
                     GGGTKDPAVFSDKIFSFRRITLAPIVVLFGFAFEMYAIMKKPSEKKDKANNPTK"
     gene            complement(26799..27686)
                     /locus_tag="PLK75_03875"
     CDS             complement(26799..27686)
                     /locus_tag="PLK75_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOY38414.1"
                     /translation="MKRNKEQGIIRSRLRGAYVTSTISVSLVLFLLGIIGLLVLNAKM
                     LSDYVKENICFTLILKDDIKEPDIKKFQKTLDSYAYIKSTEYITKERAAEIMKKDLGD
                     DFVQTLGYNPLLPSIDVYLIADYANPDSLAVIEKKFASYTDYVKEVSYQKNLVHLINN
                     NVRAISVFLILFSALLMLVSFALISNTIRLMVYSKRFIIKTMQLVGATKTFIRRPFLR
                     SGLIQGVLSSVIAMALLTIILYFSMNQLREIINIQDYYKIVASLYLIVLSLGVIISVA
                     STFFAVNRYLRIKTSNLYY"
     gene            complement(27698..30736)
                     /locus_tag="PLK75_03880"
     CDS             complement(27698..30736)
                     /locus_tag="PLK75_03880"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOY38415.1"
                     /translation="MKRISKTGFFTFLLFIISFGVYSQVPQITQHPLSGEKCTGNSVS
                     FSVEATGASPLHYQWYFENNPVGTDSPILAISSVNQSNEGVYYCIVTNLSGSATSNDA
                     ILLVAGGAPVIDNYSSDTIMCQGELLLLSVEVTADYESYSWFRQGSGFVEYGQTLDIE
                     NITSENSGLYYCEISNVCGSVSTGNITVQVNDAPQFVSLPSSVEICEGNDVSFTAEAS
                     GTNIQYAWLADNVEMPGETENTLIIAGVVYPDYTFYVPVAYNICDTIYGPAVSVSVLS
                     NPVITGNPISQDLCDGEDFQLIATANSNIALTYQWYRNEDLITGETGDSYNGIAEEGV
                     IDSYYCIITNQCIWISTDTAIITGLESPVITQQPEDVLACAGEYVTLHCKASGSEPLY
                     YQWLFNGANVTGGNITGANSETLQFNEITEGQAGDYTCFVYNDCGSTLTEIATVTVNL
                     PPVILMQPDDIEVCAGQDFEVAIMVSGTNPLTYDWTNFETGVSFGTSEELVLTDIAAE
                     MSGLYICTVSNMCGDAESQTFQIMVNTLPEVLGLSENMSVCEDDSVALIVAASGTEPI
                     DYLWYRNGSAESWATNDTIIFNPAQFGNTGTYFCRMYNLCGQTDSDPVELYVGTPPSI
                     QWNTGTQYLCEWDTLNLVVDPAGEFVAFQWYFNDVIIDGATDSVYNVPNITPGMTGMY
                     TCRVYNSCDEIFTSEIEVNINPAPEVDLGDDIETCEGISVVLHPEGDFQSYTWNDGLS
                     SGPQLDVEISGTYWVVVVGQNACSNRDTIHVEIHPVIEVDFGNDVSACGEYLLTAPEG
                     AYSYQWSTGASGVNEILVTESGPYTVTTTGDSFGCQSVGYIEIEIFEIPVFNLGPDHI
                     IANDSAITVGVETEYADYIWYNGFNGPMLTVSGADLGTGEHDIWLRVMSENGCNFTDS
                     IVVSVFDASGIENLGFGGLTVCPNPASGLIFVSADDAITGQALLEIYDTRLNIVYSDK
                     QNLYPMWQRAIDISNLSPGVYFIHVLFDSGKRVTKKISIF"
     gene            30924..31274
                     /locus_tag="PLK75_03885"
     CDS             30924..31274
                     /locus_tag="PLK75_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY38416.1"
                     /translation="MKIAYSIVLILLISVVTAFAGNDNTDADKTQSNGVISGQVVDKL
                     TGEGLGGVKINFSNGIELYTDLNGNFELIVTCNNSFQLQTSYISYETFTIANLHINPG
                     EKQVLRVEMKSIED"
     gene            31319..>31703
                     /locus_tag="PLK75_03890"
     CDS             31319..>31703
                     /locus_tag="PLK75_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38417.1"
                     /translation="MSKPLFRNTFILLFFQLSCIFSFSQGVKNIDGLYYTDKNELYTG
                     DYIELYDNGNIKLKMHIENGQKEGETMLYFQNGSVSEARNYHKGKKHGDWVCYDTSGV
                     KIAVAQYENNQKSGLWLIWDANGILR"
BASE COUNT         9593 a         6530 c         5939 g         9641 t
ORIGIN      
        1 tgccttcatc gaaataacca gctgataaaa accttccgct acaaatattt taatatactc
       61 aatcgcagct tcaaccattc cttctactgt gttgccatag gttttcagaa ttctttcaga
      121 taatgacccg tggtttgagc caattcttat agcagtacca tattctttgc aaatactaat
      181 aagcggcttg atgcgagaat gtattttttc caattcaaac ctgggttcat ctacctggct
      241 gttcaaacga tggtgatttc tatcgtagta gttacctgga tttattctaa ccttctcaac
      301 aattctggca gctgtttcag caataacagg gttgaagtgc acatctgcaa tcaaagggtt
      361 tgtaaatcct ttctcttgta ggagtttttt tatttgagtc aggttctcgg catcggcctg
      421 agttggtgtg gttatacgaa cataatcggc acctgcttca aataatcgga tacattgatc
      481 aaccgtgttt tgcgtatcgc tggtttgtgt attggtcatg ctttgcaacc ggatgggatg
      541 cattgcgcca agtggaacgt gcccaatctt tacctctttt gacgggaaac gttcataatt
      601 ggctaagtcg atactatatt ttttcattta aaaaaattac aacatatagc gcatttttac
      661 gcgtattgct gtttcttctt ttttgacaaa ctttgctgat tcaaactttg gaggaccgaa
      721 ggtaactttt gcatcgttcg aaaatccaaa accctctttg ggaatcccaa cagcactcat
      781 gtccaaatcc ccattgttat tttcatcgtg aagaaccgaa atagcataga tcccatcagg
      841 aacacctttt attagtattc gcaccgtgct tccatgaact tcgaccaggc cctccttaaa
      901 gatatcggta tgcgagttgt actcattttt ttcgttatac agtgcatagc gaacaacccc
      961 ttttgtgctg cgtatactgt taatggtaac aagaatgcag tcggtctgag aaaataccgg
     1021 taataaaaag aaaagaaaaa aaccagtaat tattattccc ctcatagcta aaaacaatta
     1081 gtaaatatca atgctaaaat taaaaaaaac ttgtaataaa atagtatttt cgttgctttt
     1141 attgaataca tgagcaaaga aaaggttttt atagcaggag cagccgggtc catggggttc
     1201 gagactttta aatctgtatg gaataaatgt gataccaatg gcgaccgccg tttcgacatt
     1261 gttttattgc tcttcgattc ccgaaagaac agaaaactgc ttgctccata cctttttgaa
     1321 tgtggaattg ggttctcaag aagtaaaatc atcgaaaaaa acggacttaa ggtaatatgg
     1381 ggcgatggta gaaaatacga agacattcaa aaagcagtca gtggggttga tttcgtgtta
     1441 aattgcatgg caataatcag tccacgtgct gaaaaagagc ccgaaaatac aaaaaacaca
     1501 aacctgaaag ctgttgaata tattatcaag gctattaaag agcaaccgaa cgggactgaa
     1561 catatccggt ttgttaatgt gggcagtata gccgaatatg gcgacagaat gggtaggata
     1621 catgcaggca gggtgggaga tcctgtagtt gttagccctt ttgatatata tgccattaca
     1681 aaagtaaaag cagaaaggct ggtgattgaa tccggattaa aacactgggt tcagctacgg
     1741 cagactttta ttatgatacc gaacgtgttc tcgctcgaag accccattat gtttcatcag
     1801 ccgattggtt gttttatgga gaacataaca gcaagagcag ccggcaaagg catggccaat
     1861 tgccttgaaa taccccataa ttctgatttc tggagaaagg tttataatat gggtggcgga
     1921 ccggagtgtc gcacaaatta tcttgacttt ttaaacgata tgtttgggtt aatgggatta
     1981 cgctatcaga aaatatttga gcgaaaatgg tttgcattac gcaatttcca catgcagtat
     2041 tatgccgatt caaacattct taatgaatac cttaaccatt ggggcgatac attagatgac
     2101 ttcaaaaaat cggtatctca taatctgcca tggtatctga aaacagttgt taagctcaat
     2161 aaatcagtac cggccttcag gaatatggtt gaaaaaataa cacggcgcag aatgaaaaat
     2221 atggctatgc gcagaaccgg taacctaagg tggattaccg agaataacga ccttcgaatt
     2281 gcagcctttt acggctcacg cgatgcctat gatgccattc cagactggga cgctcctctc
     2341 cctgaaggac ttgctgggac ccccgagcca gaatgtattc aactgaatca tggctacgac
     2401 caaacaaaag aatcgttgga agtatccgat ttacaaagcg ctgcaaattt cagagggggt
     2461 aaattgattt cggaggaatg ggatcaggat atgtattgtc aaatggtttg ggaatgtgaa
     2521 tttggacatc gattcgaagc cagtgcctat gctgtgctga aagcaggcca ttggtgtacg
     2581 gtttgtgagg ctccgccatg gaattacgat gaaattgccc gaaggaatcc gttttttgca
     2641 caggtttggc atgaatcaca ctcccctgat gaagaccatt tttaccccga attgtgttac
     2701 gacgatatat tataaaatgg agttcaatta tatatattat aagtactacc tactgtttat
     2761 gacagtactg gcggttgttg tgtttgtctc tcttcagttt gttgaagccg gttatggaaa
     2821 attcaaaaat aaaaagtggg ggttcagcat taaaaataag attggttggt ttctgatgga
     2881 aacaccggtt tttattgcca tgtttttact ttggcttttt tcggaaaggg ggttcgaaat
     2941 tactccgctt attatctacg tcatttttat gtcgcattat ttccatcgcg cttttatatt
     3001 tccgtttcgc ctgaaaggta aaaataccat gcctgttgcg attgtactca tgggagtaag
     3061 ctttaatatg ctgaatgcct ttatgcaagc gggctggcta ttctattttg ccccttccga
     3121 catgtatagt gtttcgtggc tttactcacc tcagttcatt gcaggtttta taattttcgt
     3181 agccggaatg tttgttaaca tttcatccga cagtatcatc aggaatctgc gtaaggaagg
     3241 cgacaacaac tactatctac ccaaaggcgg catgttcaac tatgttgtat cggcaaatta
     3301 ttttggtgaa tttctcgaat gggttggatt tgcattatta tcatggtcgt gggcggctgt
     3361 tgtatttgcc gtatggacat ttgccaatct tgcacccaga gcaaaatcga catatagaaa
     3421 atattcgtta ctctttgggg atgaatttca cagaaaaaag cttaaataca tcatcccttt
     3481 catatattag caaggcccaa ataacaacaa aaacaatgtc gatagaagta aaaaatatca
     3541 ccaaactttt caagaaacaa aaagcactcg atgatgtttc tttctcaata cactcaggag
     3601 aaatagtcgg ttttctggga cccaacggag ccgggaaatc aactatgatg aaaatactta
     3661 caggttttat tcctccgaca tcgggagatg cttacattca tggtgtacat gtattggaag
     3721 attcaataca cattaaacga ctgattgggt atttacctga aaacaatcct ttatataccg
     3781 atatgtatat cagggaatat ctcaattttg tggcaggtat ctacaaaatt ggatcaaacc
     3841 gttcgaaacg catcaatgag gtaattgagc tcactggtct ggaacccgaa ataagcaaga
     3901 aaataggagc actttcaaaa ggattccggc aaagggtcgg gttggcacag gcaataattc
     3961 acgatccaca ggttttaata ttggatgaac ccacatctgg gttggacccc aatcagatta
     4021 ttgaaatcag aaacttaatt tcggagttag ggaagaaaaa gacggtcatg ctttcaaccc
     4081 atattcttca ggaagttcaa gctatttgcc agaagattat cataataaac aaaggcaaga
     4141 ttgttgccaa cgaagatgcc gcaatattac aagaagaatc cgcaggaaca aaaaaatcga
     4201 tacatattgt tttcgagaca cccgttagca aaaaatccct cactgaaatc aacggggttg
     4261 aaaaagtagc ttccttaagt gaatgtgaat ttgtattaca gtcatgcagc aaaaaggaca
     4321 tacgcccgga gataatgaaa tttgcagtta aaaacaatct gaatatcctt agcatggccg
     4381 agcaaagcaa aagccttgag caggttttcc aggatcttac caaataaaaa tcgcatatag
     4441 attgaatttc aatcattatg cgattctctc ctgcccacac ataaaattat ttctccatca
     4501 atgtcaggct tcggcttaca aaccttgtta gagcttctcc cttgagcata ttgttcgaaa
     4561 gtaatgcaag gtcgaataac tgacttatta tttggcgact ctttccaaaa ttacgaagta
     4621 agttcgagcg attttcgttc attgcatcaa cctgttcctg taactgtatc ttttgttctt
     4681 tctcttcggc aggaatatca gcatccttct tacccttatg cttttcagta agctcttcaa
     4741 gtagtttttt aagtggctca actgagctgc tgatctcaat gagggtttca ccgagttcac
     4801 tttcggcttc acgacttaat tcaacaatta tcgagtggtt cgaattcact accaaccgga
     4861 agctatcggg catatcacga taaaaactgg taccactacc gaaatgcgac atatccttca
     4921 tgcgacgcat aaactcatca cgggttaata acaccggcat atcactttca ttcatatttt
     4981 caacgcccac aatatattta tcgttttcgg gaagaacacc ttcaaacatt agtcgtatac
     5041 gttgtttttc gagttcactg agttttgact cctctgtctt ttccttttta accagattat
     5101 cggctgtttc tgcatcaacg cgaacaaaat gtgagttttt aaacttagtc tccagatgat
     5161 taataaagtg catatcgagt tgaccatcga aaagaagcac atcatatccc ttgtttttag
     5221 cagcctgaat gtagctgaac tgtgtttcac tatcggtggc atacaggtag accactgttt
     5281 tgtttttatc cgtctgttcc agttcaatca gttgcttata ttcctcgaaa gtaaagtact
     5341 tcccatcggt atttttcagc agtgaaaact tttcagcacg ttcataaaac ttctcatcgg
     5401 taatcattcc gtaaatgata aataatttca ggtcatccca ttttttttca aaatcggctc
     5461 tgtcgttttt aaatatttct gccaacctgt cggatacttt tttcgaaata tgacctgata
     5521 tttttttaac attggcatca ttctgaaggt aacttctcga aacattcagt ggaatatcag
     5581 gtgagtcgat aaccccatga agcaatgtga ggaattcagg aacaataccc tcaacggaat
     5641 cggtaacgaa aacctgattg cagtaaagtt gtattttgtt tttctgaata tcgatattac
     5701 tttttatttt cggaaaatac aaaacaccgg taaggttgaa cgggtaatcg acattcaaat
     5761 ggatattgaa taatggctct tcgaaagaat atgggtacaa ctccctataa aatccagcat
     5821 aatcttcatc tttcaggtct gctggctttt tcgtccatac cggttgggtg ttattaataa
     5881 cattccactc atcggtttct gtttctttgc cgtctttcca ctcggtcttc tttccgaaaa
     5941 caatctcaac gggaaggaat ttacaatact tattgagtaa tgagctaatt ctgtttttct
     6001 caagaaattc ttccgagtcc ttatcaatat gtaatataac gtcggttcca atactatctt
     6061 tttcagtctc ctttaaagta tattccgggt cgccagtaca cgaccaaagt actgcctggg
     6121 cattttccct gaatgaccgg gtaataattt caactttctc cgaaaccata aacgctgaat
     6181 aaaaacccag tccaaaatgg ccaattattg cattggcatc gtttttatat ttttcgagaa
     6241 agtcacctgc acttgaaaaa gctatctggt taatgtattt ctttacctct tcttcggtca
     6301 ttccaatgcc gtggtcgcga attaaaatcg ttttgttttt ctcgtcaacc tcaatgtaca
     6361 ctcgtgcatc ggatagctcg tgagtaactt cgccggcagc agccaggatt ttaagttttt
     6421 gagtagcgtc aacagcgttc gaaaccaatt cacggagaaa aatatcgtgc tctgaataga
     6481 gaaatttttt aatgattgga aaaatgtttt ctgagctaac tccaatctta cctttttttg
     6541 atgccatatg aaatcatttt attattacgg ctttgtactt ttcaaagcac atgccatttg
     6601 aaatgatatg acatttcggc agtttttata actgcgacga aaaaatgaaa agcaatataa
     6661 ggcagcttat taggagtgtt ccaataacac caaatacata ccaggcaatt ttcatatcct
     6721 gacgtttttt cttcaggttg tatgtgctca tgatggtatt gatggttgaa taaagtcgaa
     6781 caaaggtgtg atcgttcttt ttaatataat cgaacggata cttcgaattg ctggtggctt
     6841 taacatccat gttatcgaag ccggaaagga ttactatctc agcaaactta tcgatttctt
     6901 taataattgg tagtaattcc aaaccagtac gcgcgttgat atcttcagaa ttcaaaacgt
     6961 agtcaagaat aactattttt aagtttttat ttccctgatc tcttttaaac ttatttaaaa
     7021 atgcttcgcc ggaactaaag gtgaaaagcg aaaacgataa gtcgggttta aactgtttcc
     7081 tgaaaaagct taaatacaac aggtcatcgt caacaaagta aaaatcgata tgtttcgtgg
     7141 acttcataaa tttcagtaca ttttacccat tcctgacttc atcggatatc atttttaaag
     7201 ccgttgaaaa taatggaatt tttttactat tgaaaacaag tattaataaa actatatctt
     7261 attgaacatg ttaaaagttt tttgaggctc cccttctttg taatcgatca attcttttgt
     7321 tcttttcata gttgatggcc ttattgggat cgatatcggg tttaaaatac catttcccac
     7381 tattgtattc cagggcatcg taagaaaaat caggtccata atactggtaa tgacctgtaa
     7441 atctgctatt ggagggtgcc agatgatcga tcacaattat atcgttttgc tggttatacc
     7501 gaagaatcat tgtagccatt tcgctgtact catagaataa ttttcctttt ttctctttac
     7561 ctactacaaa tactttttct ccgaatacag gcaacccgct tcgtgaaaaa ctcaacgact
     7621 ccactatttt tcggttaatc agtctgtctg ctccatccca gccaatcaga acatattgtt
     7681 ccttattatt ccatttcttt tccacaacct cataatacaa tgcaccatac cattccttgt
     7741 agctggtgaa gcttcgttta ttcccaaccg tatcgtcgga gcaatcgagc aactggtaag
     7801 taaatagctc tttgtttttc ttatactgaa gataaccaaa ataggtaaaa cttccatcct
     7861 cataaaaaat attccatgtg taaattctca gttttttgct atctgtgtca agcaccttga
     7921 gatattttac agagtcgaag ttcatttcaa atgaagcgtc atcatgcagc accatgtcca
     7981 tgtaactgat aacatagttg ttgattctga ttttttcgct atcatcattc gaaactgaaa
     8041 tctggttcaa ccatgatgca atagaatcga cagcatattg caaatcgtcg ttcttttgtg
     8101 agaacacatt atttgtaaca atgaataaag agcacaacaa tactgcagta tgtatttttc
     8161 tcggcaaatc cacaacaatg tttcgattat aacggtgcga atttaaacaa tagtattggt
     8221 aactataaat ttatagaatt gagagttttc agaatttttg cacaagcaag ctgaatttcg
     8281 tcacgggtaa tatttagagg tggggcaatt ctaaaggccg tagggtgaaa gataaaccaa
     8341 tcggttatca agccctgagt caatgcagct cttacaaatt gcattgttac ttcaaccgac
     8401 tgcaattcaa ctgcaagaag caatccttta ccccggatgg ttttaataag tttatggcta
     8461 agcagttgtc gaaatatttt ttcgcttgta tttgcattaa tccataattg cccggcagta
     8521 ataatctgca gcgatgcaag cgcagctgca cagctaaccg ggtgcccgcc aaaagttgta
     8581 atatgaccaa gcacagggtt agaagtaaaa ctgttcatta ctttcttttc agccacaaat
     8641 gctcccagtg gcattcctcc tcccagagct ttcgcaaaac atacgatgtc gggcacaact
     8701 ccataatggt ccatagcgaa cattttccca gtgcgaccga acccggtttg tatttcatcg
     8761 aaaatcaaca aacaaccagt ttcggtacaa cggctgcgca gtgctttcat gaattgcgta
     8821 tcggtttcca tcactccggc ctcggcctgt attggctcaa gtattacaca ggctgtttcg
     8881 ctgtcgatat ttatcaattc ttcgaagctg ttcaattcag ccagatgtat cccgggtacc
     8941 aaaggtcgga aagagttttt aaaatcttcg ttcccacaca aactcaaagc tccggcagaa
     9001 ctaccgtggt atgcgttttt aaacgacatc acacggttcc gtccggtata tcgccttgca
     9061 agcttcactg ctccttcaac agcttcgctg cctgaattca caaaatacac cgagttgagc
     9121 ttttcgggca acaccgagca caacatttct gcaagcttca cctgaggccc aagaacatat
     9181 tcgccatata ccatcagatg catataatcc gctgcctgag cacatacggc cttgacaatt
     9241 tccggattat tatgacccag attgcttact gatactcctg aaattaaatc gagatattcc
     9301 gtaccggaaa ggtctttaag aaaaacacct tgcgccgatt caatttcgag tagatatggc
     9361 gtttcggatg tctgccctaa aacctttaaa aacaactctc tgttcgaagc cattattata
     9421 ctttaacttt tgagagttca attttaccga taatagtttt ttttgccaaa cattcaaagg
     9481 gaatatttaa cgtattgcca ttgattaaaa aggtaagcgc ttcatcttca acacttgaga
     9541 ctttgttcaa attaacaagg taattagcgt gtacacgaag gaatgctggt tcaggtaaaa
     9601 aaacctccag ctcttccaaa ttactgatta ttttaaattt attttcatcg gttaccagat
     9661 aagtattttc gttatcggcc tcaataaaca atatatcatt ataatttacc ttgcggaatg
     9721 aagtacctat tttaacgaga aaattatctg agtttttttg cggataaatt tccctgtttc
     9781 tctcatatgc tttatcgacc gctttaaaaa acctttcata ggtaataggt ttcagaaggt
     9841 aatcggtaac accatattga aatgtatcta aagcgaactc ctttttaccg gaaatcacaa
     9901 taatttgctg tttaccatcg agggtgtcaa gaatttgaaa gccattcatt tccggcataa
     9961 gaatgtcgag aaacaacagg tcaatcgaat tgttttcgga ttgcaaaaaa tgtaaagctt
    10021 ctatcggatt ggagaatgac ccgctcaagg acaatgtttg agtcctttcg atgtaagctt
    10081 ctatcagatt acgggataat tggtcgtcgt caactataat gcaattcata gaaaaatttt
    10141 aacaaccttt tatacggaca caaagcaagg taagttttaa aatttatacc tatagcctat
    10201 caaaatggct aaaattataa tttgcctgat caatttcgag atagctgccg gcaaccgact
    10261 gaagtattgc tacatcgcct gttgtaataa ttctaataaa cccaaggtta tttacgcttt
    10321 tcatcagacc tttagtcatt aaaatatctt ttgtacgcct tgctactgct tccccggagt
    10381 ctaatatagc aacttcatca ccacaaattt gctgtatagt ttttttgaaa aacgggaaat
    10441 gggtacaccc aagtaccaaa taatcgatgt ttttttcgcg aagcggagaa atatattttt
    10501 gtaataacga ctcagcctcg ggagaatgaa tcatgtcttg ctcaaccaga ttaacaaggc
    10561 catatccggg ttgtgtatgt atatgaatat attcggcgta cttttcactg gtgctgcgaa
    10621 agtgttgcgc tttaaaagtt ccttcggtcg ccagtacacc tatgttccct gtttttgaag
    10681 ccaggcaggc aggtttcaca gcaggttcca gtcccacaaa atcataccca gggtatgtat
    10741 tcctgagaaa atgaatcgct gcccccgtag cagtattaca ggcaattaca ataattttgc
    10801 atttctgctt aaaaaggaat tctacatttt taacggtaag gcttactatt tcttcaacgg
    10861 gtttttcgcc atagggagca tttccgctat cacccagata gatgaaattc tcaaagggca
    10921 ttgcgtttat aagcgatttt aaaatagtaa ttccaccgat accggaatca aaaacaccta
    10981 ttggctcatc atttctcata aaaacaaaaa gaccatcaga gatggtcctt catcatttat
    11041 aataatacta ctttttctga agtttagttt taaccatctc ggtaacatcg atgctttcat
    11101 cggaataata aagtagagta gaaatatcat atatatacat gaagctattt tcctgcccaa
    11161 cctgtttaat ggcgtctttt atcttgttat aaattggttc aagcaactcc tgttctttat
    11221 tctgaagatc agtctgggca ctcatctgaa aattctgaat tctcgactga agatcggaaa
    11281 gttctttttc cttgctttgt ttaatagcag gtgaatatgt ctctgcctcc tctaagtagg
    11341 cattgtattt ttgttcaaat tcgctattca ttgcaagaag ttggtcttcg agctgttttg
    11401 catatttttc aatagccgac tttgcttcag ctctttcagg cattgcttcg aatattgcag
    11461 cagaattcac atgcccgaac tttgattttt gagcataggc tgtaaaaact aacaggatag
    11521 gtaaagtaat taaaatcagt tttttcattg ttgtctgttt ttgttatgtg taattaattt
    11581 attttgatgc aaaaataaaa tattagtttt tatatcccat tttttttagc acttcatcgc
    11641 tcttatcgta ttttggatcg gtataaagca tgttcaggct attagctgta tcgaaaataa
    11701 cagcataact tccctctaca gcaatttcct ttattgcatt ataaatatca tcctgcaagg
    11761 gttttataag ctcctgacgt tttttaaaca acatgccgtc gcgaccgaaa tatttttttt
    11821 gcaaatcgcg catttcacgt tctttattca caatttcttg ttctcttttc tctttcagtt
    11881 catcggtaag aagcaccttc tcagcctgaa aatccttata cattttatcc acctgagtct
    11941 gcatcgcatc tacttcagct ttccattgct ccgaaagctc gttcagttgc tcttgagccg
    12001 attcgtaaag cgggatgttg ttcaaaatat attctgtgtc gacataagca tatttctgtg
    12061 cccaggcgta catcgaaaac acagatacaa gcaacagtaa aatggtcttt ttcataacaa
    12121 atcgttttta gttgtttaaa taataactct ttcgttattg caaaatgtaa gccaaagata
    12181 taaaattaaa atgtttgacc aatcacaaaa tgaaattggc tgccatttgc accggttgaa
    12241 ccattgattt cgtcaaatcc ataaccccag tcaacaccca ataatccaat catgggcatg
    12301 taaattctga tacctacacc ggccgaccgt tttacgtcga aggggttgaa ttttttaata
    12361 tcgtaccatg cattccctgc ttcaacaaat gccagtgcaa acaatgttgc tgacggatta
    12421 agtgaaagtg gatatcttaa ttctacggtg aatttatcgt acacattgcc accatcgcca
    12481 ggagtcaacg agttattcaa atagcctcta aggccaattg tttccctgcc atatagattg
    12541 tacccgtaca aaccatcacc gcccagctga aagccttcaa acggagaagg gcccaaatcg
    12601 ctgttataat accccagtat tccggcttcg aattttgtaa gcagtaccaa agcccgagag
    12661 tcgcccgatt tacttccgac cagggttgta aaccatgcac cactgaactt ccacttatga
    12721 tattcaatcc acttgtactt atcgtttgca gacattgagg cataatcggc attagcaaat
    12781 aatgagtatg gaggtgtaag ttcaatcgac accgacattt cggatcccat agttgggaaa
    12841 attgggtttg gcccacttga agaccgcccg accactgttt taatgctgac attatttgag
    12901 tgaccttctt tatataaaaa tccgggatag ttatcgacaa gatagtagcg ataactcaat
    12961 tctgtgtaat tggtgaaata gtcgtcgggc caggcaaggc gttttcccaa ccccaatcct
    13021 gcacctgtta ccttaaaaga agttctttct tcggcatcgc tactaccatt tgtcgtaata
    13081 gaatggtaaa tcattgttgt tagggagttc ggttttttcc cacccaacca aggttcaaca
    13141 aacgaaaaac tatagttctg ataatatagc ccactggcct gagcgcgtat actaagccgc
    13201 tgaccatcgc ctgacggaag aggtctatag gctgccggat taaaaacatt tctgagcgag
    13261 aaattattaa acgacaaacc cagtgtacca acaatcattc ctgcaccata tccacccgat
    13321 agttcaatct gatcggaggg tttttcctca acgaggtatt ccagatcaac ggttcctttt
    13381 gccgggtccg gttgaaagtc aacattcagt ttttcaggat cgaagtagcc caactgcgca
    13441 aactcacgaa tagtacgctg cacttcgctt ctccggtaga ggctgccggg ggaagtcctg
    13501 atttcgcgca ttacaacgtg gtcgttggtg cgtgtatttc ccgaaatggc aaccttgtta
    13561 atacgtgctt gcttcccttc gtaaatttgc atctgaatat cgagagaatc gttttcggca
    13621 tatacttcaa ccgggttgat atttgaaaac aaataaccat tatcctgata caatgaaaaa
    13681 acgctttgcg gatcgtagag cagtttttct tcaagcaact tctgattata cacgtcccct
    13741 tttcttattc ccaatacctg cgataaaaaa tcggcagaat attttgtgtt cccaacccaa
    13801 gtaatgtttc tgtaataata cttaggtccc tcttcaatac taatataaac atcaattgtg
    13861 ttgttgtcga atttaacaat tgaatcggac actatgcggg catccctgaa acccagttca
    13921 ttgtattttt ctattatttt agccttatcc tcttcatatt tttcattgat cattttagag
    13981 gctttaaaga aaccatacca gcgtttttcc ttcgtttcct tcatggttct atgcaaacgg
    14041 gaatctgtga ttttttcggg cgagcggtaa aagttatacc atttcttttc tttaccaatg
    14101 gctatagctc ccgaaaagtg gatttcattt attttaattc tttcgttttt attaatgtta
    14161 atttttaaga ttacgtggtt taccatactc gaatccaagc ttgattcgat gttaacatcc
    14221 acgttaagaa aacctttttc aacaaaataa tctgttatga tgtttttagt attgattagg
    14281 gtgttttcgg ttatctggtt tcctcgtacc agcttgattt tttctttaat gtcatcagct
    14341 tccgatttac ggataccggt aaatgagaat ttcgataacc ttgggcgttc aaccaggtaa
    14401 atatcgagga atgctttttt attgacaact ttttcgaggc tgattttaat ctcggagaac
    14461 aagccctgct cccataactt attaattgcc gctgtaattt catcgcccgg aatcataatt
    14521 acatcaccca cctgtaatcc ggtcaaatga acgagtacat ccggatcaag atatttaatt
    14581 ccgcttattt gaattccccc gatttcgtat tcgaccggat cggaataatc aagtttttta
    14641 atgcgtgacg aaatgtcttc ctgggctgta acactagtaa ccaagtaaac aatgaataat
    14701 aggaatgcga tttttttcat gcgccaaaat tagtttttta tttgttcact tgttttacca
    14761 aatcgtcttt cgcgattttg ataattatat atggcttcaa acaaggtttc ttttgtgaag
    14821 tcgggccaat gcagtggtgg aaaatagaat tcggaatagg ctaactgata caacagaaaa
    14881 ttgctgattc tcatttcacc actggttctt attaaaagct ccgggtcggg aatatcggct
    14941 gatgtaaggt atctggcaaa aagcgactca ttgatttcat cgggggtaat actatggtta
    15001 caggcatcgg ttgcaatacg tctggctgct tccgcaattt cccatcgccc ggaataactg
    15061 agagcaacaa gtaactgcag gttactattg ttttttgttt tttcacaggc aaactgaagc
    15121 ttctgataca cctttcgtga tagacgtgac atatcgccta atatcctaag gcaaactcca
    15181 tttttatgta gctcttctat ttcgctttca attgaattaa ccaacaaatc catcagccct
    15241 aaaacttcgg tctgtggcct gttccagttt tctgttgaaa aagcgtataa ggtcaggtat
    15301 tgaactcctg cttcacgggc tccaataata gctcttttca ctgcctgcac accggcctta
    15361 tgtccaaaca tccggggttg ccctttttct tttgcccaac ggccattccc gtccataata
    15421 atggcaatat gtactgggat tcgggtttta tctaattttt ccagtaaact cataattcat
    15481 ttaataaaca tcgtaggcag ggcacggaaa acctccccag ctgaaacaat atgacaaccc
    15541 cagactggca tatgagtacc agtccttatt catcgcaaat ccgatttgcc gaatccctgt
    15601 tgtggcgtta taaggaatat tatcaacagt attgtattga tataaatcct ctccggtagt
    15661 attgtcgagg aggtcggtaa aagtccttct gaagccccat tctgcagaga gaaccattct
    15721 atgtgagata tttaccttaa aacccaatcc cacaggtatt gaaaggccgg taacttttgc
    15781 agaaccgggt gtgagataat acgccaagcc tgtttgcagg taaggtgtat agtttcgttt
    15841 ccgtaattcg cccattaggt agggaagaaa attaaactcg gtttgcagcg aaatttccat
    15901 catgtttgta gaaaaccttg aatttctcaa tttctggtaa tatgaattga aatcctgatc
    15961 atccgctgat agtgagcttg caaaaaatcc gactctaaca ctcaatctgg aattaaggtg
    16021 atgtttgtaa aaacctccaa gattcaggtg gggttgataa aacgggattg ccaggtttat
    16081 atcacccatg taataagagc cacctgccat taccccgatc tcgcccccac cactgcgctg
    16141 accgaaagcc gctccgtgaa taagtgaaga caatatcagt aaacatccgg ttattaattt
    16201 agtaactggc ttcatacatt cgaataatta catcaatcat cacaatcaat aaatcgagca
    16261 ttgcaattac aacgtacaaa gcagggaaaa attacaatga gattaaaatt tcggcatgcc
    16321 gggttgacgt ggccttaact tgtacgaagc attaatggtt aaaaacatgt agcaatcggt
    16381 gtaatggtcg tcgcctcttt gttgcagtgg accagttcca atccaagtcc caagagtggg
    16441 gtcgctgaaa taacgggcaa taggatcttc gaaaaggttc ggatcaacat aggttgtgct
    16501 cacatcgtcg acataatcgg agaatgtata acggaaaccg tattcaaagc caattcccca
    16561 gttgcggtcg agcatgtagt tcatcccaaa tcccataggt attgcaatgg caacataacc
    16621 gtatttttcg cgggttggaa ccaacccctg cccttccgtg ccaagaggac gcaatgcgta
    16681 ccattcgcca tcggtatatt ttgctttggg attgaaatag aaagcactga taccagtaaa
    16741 accatagaga tttggcaggt ttttcttacc tttcaccctg cgtaagtcgt aacgatggcc
    16801 atagcgttcc ttgataattg aatattctat atttgctgtc cattccaaca ccggtgagcg
    16861 gaaagacaaa tctctgtcgt gtctgtcggg ttgaagggtt aaactatcgg caccggagag
    16921 ccacccatac caaagtcctg ttttaacagc ccagtccggc gcaagcttat agcggtagcc
    16981 aacaccaaaa actgctcgtg tggctttaat ttcaaagtcg cgcaaaaagt gggtaccaat
    17041 ctgatttgca ccacccaatt ctcccagaaa gttggttggt ccggcaccaa aataaatttc
    17101 atgacgtatt tttttccagc ttgtgcctaa ttgggcattc actacagctg agcagaaaac
    17161 actaaatatc aaaaggaaaa atattttttt cataaacaac tattttctaa ttttacatta
    17221 gcctgacaat taatttctta catcaatacc ccacatcaac ttctctcgca gggtgctaaa
    17281 ataataattt tgtgacaaag ttacaacatt aatatgaaaa tcagactttt ttattgaaaa
    17341 ttcaacattt gacccaacgg ctttggacct gtaatcgagg gtaacaagaa tttcccgaac
    17401 cgggccctcc acttgtagct ttatttcgct gtttgcagga attaccaaag gtcgtagcgt
    17461 aagattatgg ggagcgattg gagtgatgac aaagcaatcg gcatcaggag tcattattgg
    17521 acctccgcaa ctcatcgaat aggctgtaga gccagttgga gtagctatta tgataccatc
    17581 tgcccaataa gtgttaatat attctccatt gcaaacaact tttattttaa tcatatttaa
    17641 tgtgtgtagc ttttgcgtgg taaactcatt caatgcaaaa gaatatttct caaatatcgg
    17701 ttgctgcgta ttgcactcca acaggaatcg cttagtcaat gtatattttt tttccacaat
    17761 ctgcttcact gtgccgagaa cctgatcttg actaacattg gccaggaacc caagttttcc
    17821 ggtattaata cccaaaaccg gaatattgcg attataaaca caggttattg tttctaaaaa
    17881 agttccatcc ccgccaacac tcaacatcaa atcaaaattt tcattatcag gtaatttgtc
    17941 gtgaaacaca ccagataata ccggtttgta catcaggttt tcagtaataa aatgcaagaa
    18001 ctgatcattc ataaaaatct tgcaccctgc atcatgtagt gcttcaaaca gttgaatggc
    18061 tgctccattg aattttttcg gaaaattccg accgtaaact gcaacattca tggttagtat
    18121 tgctttaata tcggggtttt aaactgaaca aatatattaa acttttcggt caggttgtta
    18181 tacgcgtaat aaacactcaa aaccctgtcg tagtaagtca ccaattcgaa gccagcgccc
    18241 agaccagcaa caatattata gtttttaaca taccctaaat cggcaaaagc agtgaggaag
    18301 agttcgtagt gaattttatc gaattgttcc aatttcaaca agtttagttc tttgtacagt
    18361 tcgcgaaaaa ctgcgtacct gaatgaactt ttacacaaca gatagatttc ggaattcaga
    18421 acaaaattat cggcccctct taggtaagtt tcgtaaccca ggctgcgata caaaccaagc
    18481 gggatacttg tatttttgct atggtgcagc aaggccatgg tctcgatgcc agtaaaaaac
    18541 ctttgcgaaa attgaaaaaa cacgtttgct tcggcattaa aaaccgtttg atttatccat
    18601 ttatccgtat cggtaaaagg tatcccgtac tgggttattt ctgcacccgc aaaaaagccg
    18661 gatgttggga aatacttgtt gttccgtgtg tcgtgctcaa atttgatcga aaattcggtg
    18721 catgaaaatt cgttttccga aagattcaac ctatcaggtg tgcaccatgt atttctatag
    18781 aagttatggt aagccagttt tgccttacac accgagttgt aaaacacgcg gtaacttact
    18841 ccggcttgaa caccaaattg atgataaaga taatttggtt cactcgattc gtaaacgggc
    18901 atgtaatcgc gaaatccggc aacaatttcc ttttttctaa agaaatctcc ccctgcccaa
    18961 tagccgaatg atttcctcct gaaagacccc ggattggaga aataaattga atattgttcc
    19021 ttatacccaa accgaatttt cgctttcagt aaaaggtttc gccccaatac attatgcttt
    19081 tcaaacgaaa caccgtaatt cagtcgctcc cactgccggt tgaagaaata ggtgtcaaaa
    19141 ttctgatcgg caagttcaaa aattgggtat atccaaagat accaccgctc aacaagtgaa
    19201 atgaaaaccg aaacagaaga tccatttaca gaatatgcaa tatctgtaaa attaaaaaga
    19261 cccagtagcc gcaacttatt ttcggagtgc cttatgcttg cttcgagcaa ctcttgtgta
    19321 attgtatcgc ccttcgcaaa ttcaagctca tcgagaatga tttcaggaac tgttttttga
    19381 tttcccgata tgataataga atccacaaca taccattgtt gcgcctcaac atattgcacc
    19441 aatgaaacac aaattaaaac gcatgtaaaa aaatatttca ggaataccgt tttcacatgt
    19501 tcagataatt cataaatgaa tcgaaccgac tggcaaggtg agcctgagtg ttttcctcgc
    19561 tcaggaaata tgccacaaca ctataattat atcttgaaaa agtttgcagt accgggctga
    19621 tatcggttgt atcaacttta agcgtaatgt tcattttggt agtatcagtg cttgccgaca
    19681 taaacaaact gagtatttta atattgttcg attccactat ctgagcaatc tggcttaacg
    19741 aataatcgtg aatattcatt tcgagcacaa tgatggcacc agacttattg atgtttacca
    19801 ttttcacgaa tgcaaacacc agatcctttt ttgtaattac accaaggaaa tgcctgtttt
    19861 tatcgagtac cggaacaaca ctgattctta ggttatcaac aatttcaata acttcgaaaa
    19921 tatgttgcga atcgaaaaca aacggtttta tctggctgag cttatgaaaa ccaattgggt
    19981 cgctaaggca gttgtggtcc aaaacatcgt tatccgatac caaaccaagg aactcctcat
    20041 tattcacaac aggcagatgc gaaatacgat aggtatccat ccacaacaat gccttttctc
    20101 cggtatcgga tgttttcaaa cagggaatag cttctgatat taactccgat gctatcataa
    20161 aaatgttgtt acaattaaaa ttattagcta aacaaattaa tacctttgtg ccatgaaaaa
    20221 caagaaaaaa caatgacaaa actgagtgta aatataaata aaatagcgac gctacgaaat
    20281 gcacgcggag gaaatattcc cgatgtatgt gaagcagtta ttcgttgtca gcaattcggg
    20341 gcagaaggca ttaccgttca tccgcgccct gatgagcgac acatacgata cagtgatgtt
    20401 ataaacataa aaccacttat ttcaaccgaa tttaatattg aaggctatcc atcggaaata
    20461 tttatggagc tcgttcttca ggtaaaaccg actcaagtga cacttgttcc cgatgctcac
    20521 gatgtgttaa catcaaatgc aggttgggat acagctaaac accagatgtt tctaagtgcc
    20581 gttataaaat tacttaaaga caatgggatt aggacatcaa tatttgttga tacttctgag
    20641 ggaaacataa cttatgcggc caaaacagga acagataggg ttgagttgta cacagaacct
    20701 tatgcatcgg aatataaaac caacagggag tctgccatag cccgttttat tgaggctgca
    20761 aaacttgcgc aacattttgg actgggagtg aatgcaggtc atgatctgaa tcttgagaat
    20821 ctacaatatt ttcataagca aattccttca ctgctcgaag tatctattgg tcatgcactt
    20881 atttcggatt cactgtacta tggtcttgaa aacaccattc agatgtatct gcgggcgttg
    20941 aagtaatgta aatcaataaa gtgaatggtt ctaactaaag atttttgttt gtattcaaaa
    21001 gctcttcaat ataaaaccgg ttgttcgaca ggcgcggaac tttattctgc cctcccaatt
    21061 ttcctttttg ttgcagccat ttaaaaaaac aaccttcttt aagcacattg tattttggaa
    21121 actccagcgt gatatcctta tatcgcttgg cttcatagtc ggaattacaa tgttgtagct
    21181 caatatcaag aatatacatg aacttttcga ttgaatcggg aggtgtaata aactcgaaaa
    21241 gccattcatg tgcaccctta ttattctgcc ccatatacac cggggctgcc gtaaattccc
    21301 tgacttgagc accagtgcgc atacatgcca cagccagagc tttttgagca ttgtcaacaa
    21361 ttagttcttc gccaaaggca ttgataaagt gcttggtacg cccggtaatt ttaattttgt
    21421 acgggttttt cgatgtaaat ctcactgtgt cgccaatcat gtaccgccat agtccaccgt
    21481 tggtagaaat gagcatggca tagtttttac cgcattccac atcctcaagc ataactgtat
    21541 tatcagcggg ttccccgtat acttccatag gaaggaattc gtagtaaata ccatagtcta
    21601 gcattaaaag catatcgtcg ctcgacaggt catcttgtat gccaaaaaat ccttctgaag
    21661 cattatatgt ttccagatat ttcatgtcgg aagaagggat tatgctgcga tatacctctt
    21721 gatatggcag aaaatttacg ccaccgtaaa caaaaagttc cagatttggc catacatcac
    21781 atatattatc tgatccggta tattcgatga tttttttcaa cagaacaaga aaccaggagg
    21841 gcacaccaat aaggttggta acattcttat tccatgtggt ttcgaccatc atcttcattt
    21901 tttcttccca ttcatccatc agagctattt cgggttttgg gaccctgtta aaatatgtcc
    21961 agaatggaag attccgtatc aataccgctg aaagatcgcc ataatatgaa ttttcactaa
    22021 gggtattcac cgtagtgctg ccaccaagtg tcagtgtttt accgctgaaa atagtggtat
    22081 caggaaaatg atcggtaaaa attgcaaaaa tatctttccc gctgcggaaa tggcaatctt
    22141 cgagcgattc tctgctaatt ggaataaact tgcttttatc ggatgtggta ccgcttgatt
    22201 ttgcgaacca ttttatttcg gaatgccata atacattttg ttctccattc ataatacgat
    22261 gaatgtatgt ctttaacttc tcgtaatcgt gtacaggaac tttttgaaag aagtcagaac
    22321 ggctggagat gttcttaaaa tcgtattttt ttccaaaaac agtatcgctg gctttttcga
    22381 taagttgttt aaatactttt tcctgaactt cgatgggatt tcggcgaaaa tattcaattt
    22441 cgtcgagcct accgtagtta atcagattca atatagaatt taccagtggc atatattaag
    22501 ttaatggcca aaaatacaca ttttttatat cagatttact gtgttcaatc atgtgttttt
    22561 atacttttaa ataatagatt atttttgttg tatggagaag gttttttctg gcacattgcg
    22621 taaacttcaa acacgatttg ataatcctgt tcgctacagt atctataacg aattgcatga
    22681 caccagggtt aacccgctaa taggtagaag tattcgcatc agccacaatg gagtaataaa
    22741 ctgcatacat tgtggtgcag aaacaaaaca atcgtttcat aacggatatt gctacagctg
    22801 ttttattact cttgccgaaa ccgatgcagg agtgcttcac ccggaacttg ataaatcgca
    22861 tctcggaatt tcgcgggata tggaatggtc gaaagccaat tctttgattg atcattacgt
    22921 ttacctcgct gttacaggca atcttaaagt tggtgttact cggcacaccc agataccgac
    22981 ccgatggatc gatcagggag caatcagtgc tgttaaactg gctaaaacac cgtacagaca
    23041 acttgccgga caaattgagg tagagttgaa aaaatatgtt tccgataaaa caaatgtagc
    23101 cgcaatgttg caagccgtaa cgcacagtat tgatttggtt gcacaaaaaa atttgctgtc
    23161 ggagcacctt ccggaagagc ttcagcaata cgttacactc gataaccgga taacccaaat
    23221 catttaccct atacacggag aagtagcata taaaacaaac ttaaaacttg caaatacccc
    23281 acagttcgaa gcaaagttga ttggtgtgaa aggtcaatac ctgattttta caggcggaat
    23341 tgttttcaat gtcagaaatc atagcggtta tcacatttgt attgaattgg agtaatatgg
    23401 aaaatataaa aatgaaagaa gcgctgaatg cagcgaaatt gcgttgcagt aaaggcgaaa
    23461 aatgcatatc cgaggtcgtg gattttttag ccagattcga cataacagaa aaggaaaccg
    23521 caatgataac cgccatactt attgctgaga agttcatcga cgaatcgagg tatgcacggg
    23581 cttatgttct cgataaattc cggtttaaca agtggggaag gataaaaata cgatttgagt
    23641 tgcagcgtaa aaacatacct gctgccatta taattgattc gctagattca atcaatgaaa
    23701 aagaatattc cgatgtaata aagtttctta tttcgcaaaa gaaaatcaaa acagacaacg
    23761 cctacgaata tcgtcaaaaa atgcttcgtt atctgtacgg aaaaggttac gagcccgaaa
    23821 tagtggagaa attcataaat tgcccgtaag gaagattata ttatcagcat tgcgtcgcca
    23881 taggtaccaa agcggtattt ttcctttatt gcgattttat aggcgttcat caccatgtcg
    23941 aacccaccaa aagcagcaac catcatgagc agggtggtaa aaggcaggtg aaaattgctc
    24001 accagtctgg taggaacctg aacatcgtaa ggtggaaaaa taaatttatt tgtccagcca
    24061 ttgaaaggtt ttagcatctg attcgtagta actgaacttt cgagagtacg caacacggtt
    24121 gttccaactg cgcaaacatg tctgcggttg agttttgcgt tattaaccat atcaacggca
    24181 tcgtcaggga taattatttg ttcactatcc attttatgct tcgtgagatc ctcaacatca
    24241 acatgcctga aattacccaa tccaacatga agggtaacaa aagcaaactg aacaccaatg
    24301 atttcgagtc gtttcataag ctcacggctg aaatgcattc ctgctgtagg agcggctaca
    24361 gcaccttcat gctttgcaaa tatggtctga tatctttctt cgtcatcgtc ttccacaggg
    24421 cgttttataa acttgggtag cggtgtttca cccagttcgt acaaggattt tttaaattca
    24481 tcataagagc cgtcaaataa aaaacgtaga gttctgcctc tcgaagtggt gttatcaatt
    24541 acctctgcaa ccagggaatc atcagggccg aaatacaact tgttgccaat gcgtatttta
    24601 cgggcagggt cgacaagaac atcccacaag cgctgatctt tgtttaattc ccggagcaga
    24661 aacacttcga tgcgtgcacc tgttttttcc ttgttaccat ataatcgagc cggaaatacc
    24721 tttgtattat taaatacaaa tacatctccg tcttcgaaat aatctttaat atctttaaac
    24781 atacgatgct cgatattccc ggtttttcta tcgagaacaa gcaaccgcga ttcatcgcga
    24841 ttatcgcatg gatgcaaagc tatcagctcc tcgggtaaaa cgtacttaaa ttgcgataat
    24901 ttcatttcaa tttaaaataa actgtatatt ttcagtataa atctctatca ttcggtaaaa
    24961 cgttcactta tacataaata gtttgaaaaa ggttacatat tcctaataat tttttctatt
    25021 tcatcgattt cgtaagcatc ttctaaggag aaatctcccg actttgtcct tatcaaacct
    25081 gttaaatatg ctccgctatt cattgcagca ccaatatctc gggccaatga ccttatataa
    25141 gtacctttcc cgcagcacac cctaatagta acaatcggca aattaaaatg ttccaacgaa
    25201 atttgagtta ttttaatggt gcggggtttt atttcaggat ttttaccttt acgggctatc
    25261 ttatacgctc ttttaccctg aagcttcact gctgaaaaat gcggtggaat ctgctcgatt
    25321 tcaccgacaa agtttttcag gcaaataatc agggcatctt tagtaatatg actgaattca
    25381 aagcaagcat ccatctctgt ttcactatcg aaactgggag tggttgcacc gaacttcagg
    25441 gttgccagat attctttatc gaaatcctgc aaactttcga tttctttggt agctctaccc
    25501 gtgcaaatca ataaaagccc ggtcgccatc ggatcgaggg tgccggcatg gccaactctt
    25561 aacttatcga catgaatttg cttacaaata agctgtcgca acttatttac cacatcaaac
    25621 gaagaccagg caagcggttt attaaccaag ataagttgcc ctgccatgaa gtcaacattg
    25681 cttccgtcga atagttgcat aatctaaagg aataatatgc ttaccaaccc aattacagcg
    25741 caatacacgg caaaccagat aagttttccg cgctgaacaa ttctaatcat gaagctgcag
    25801 gcaaaccagc cgcttataaa tgcagcaatt gacccgacaa tcaaaggcat tatttcgatt
    25861 ccactactcc ctgagaagtt tcctgaaaca atttccagaa aattttcccc caaaatcggg
    25921 atgatgacca tgagaaacga aaattttgct atatcttctt tcttatttcc cagcatcagt
    25981 cctgtagaga ttgtagcacc cgatcggcta ataccgggga caacagcaat ggcctgtgcc
    26041 actccgataa taaaagcatc gaaataagaa atcggttttt gtttgggttt aaaaaagtat
    26101 gtaaaagtga gaagtaaggc agtaaccaaa agcatcaacc caataaatac catatttgaa
    26161 ccatcgaaca aggttttaag ataatcttta aaaagtaaac ctattaccaa aacagggatc
    26221 atcgatactg caattttcag caaatagtgt gtctcttcat taagtttaaa tttaaaaaca
    26281 ccgctgacga gccgggtcaa ttctttgcgc aaaaccagaa ttgtgctgag taccgtagct
    26341 ccgtgcacag cgatggagaa ataaatattt tcttctcctt ttatattaaa aatggcttta
    26401 ccaatttcca ggtgaccgct actgctaacc ggtaaaaact ctgtaagtcc ctgaacaagt
    26461 ccaaggatca atgcttcgaa ccaactcatt tgaattgcaa ttatttagtc ggattatttg
    26521 ccttatcttt cttttcggaa ggctttttca ttattgcata catttcgaaa gcaaaaccga
    26581 ataatactac aattggagcc agggtaatgc gccggaaact gaaaatctta tcgctgaaaa
    26641 cagcagggtc ttttgttccg ccaccggcca tcagtacaaa accgatcaca ataatgataa
    26701 agccaatcag caacaaaata taattttgct tagtgagtgc aaattcatcg gcattgttct
    26761 gcttcggcgg gatattcgtt tctttcttca gtgccatact aataatacaa attagaagtt
    26821 ttaattctta aataccggtt gactgcaaaa aaggtagagg caaccgaaat tatgacgccc
    26881 aaagataata caataaggta taaggatgca actattttat aataatcctg aatatttatt
    26941 atttcacgaa gctgattcat tgagaaatac agtataatag taagcagtgc cattgcaatt
    27001 acagagctca aaactccctg tatcaaaccg ctacgcaaga atggcctccg aataaaggtt
    27061 tttgtggcac caaccaactg catggttttt ataatgaatc ttttcgaata caccattaag
    27121 cgaatcgtat tacttatcaa tgcaaatgaa accagcatca gcagtgcgct gaataaaatg
    27181 agaaaaacgc tgattgccct tacgttatta ttgataagat gcactagatt tttctgatat
    27241 gaaacttctt ttacataatc ggtatacgat gcaaactttt tttcgataac cgccaaactg
    27301 tcggggttgg cataatcggc tatcagataa acgtcgattg acggaagtaa tggattataa
    27361 cccagtgtct gaacaaaatc gtcgccaaga tctttcttca tgatttcggc agcacgttct
    27421 tttgtaatat attcggtaga tttaatatat gcataggagt caagggtttt ttgaaacttt
    27481 ttaatatcgg gctccttgat atcatctttc aaaataagcg tgaagcatat attttctttt
    27541 acataatcgg aaagcatttt ggcattaagc acgagaagtc ctattattcc caacagaaaa
    27601 agtaccagcg aaacactgat ggtagaagtt acataggcac ccctgagcct gcttcggatt
    27661 ataccctgtt ctttgttacg tttcattcgt aaataattta aaaaatgctg attttttttg
    27721 taacacgttt ccccgaatcg aaaaggacat gaataaaata cacgcccggt gaaaggtttg
    27781 aaatatcaat tgcacgttgc cacatcggat ataagttttg cttgtcggaa tagacaatgt
    27841 tcaaccgagt atcgtagatt tcgagcaagg cctgcccagt tattgcatca tctgccgaaa
    27901 caaaaatcaa tccggaagcc gggtttgggc aaactgttaa accaccaaaa cctaaatttt
    27961 caattccact tgcatcaaaa acacttacca caatgctatc ggtaaagttg catccatttt
    28021 cggacattac cctaagccaa atgtcatgtt ctccggtacc aaggtcggca ccactaaccg
    28081 tgagcatcgg accgttaaaa ccattgtacc atatataatc ggcatattcg gtctccactc
    28141 ctacagtaat ggctgagtcg tttgcaataa tgtggtccgg ccccaagttg aataccggga
    28201 tttcaaaaat ctctatttcg atgtaaccca ccgactggca gccaaagcta tcaccggttg
    28261 tggtaactgt atagggcccc gattcggtga caagaatttc atttacccct gatgcacctg
    28321 tactccattg atagctatat gcaccttcgg gagcagtaag aaggtattcg ccacaggctg
    28381 aaacatcgtt gccaaagtcc acctctatta ccggatgaat ttctacatgt atggtatcgc
    28441 ggttgctaca ggcattttgt cctactacca ctacccagta tgtcccactg atttcaacat
    28501 caagttgcgg gcccgaactc agaccatcgt tccatgtata gctctggaaa tcaccctcgg
    28561 gatgcaaaac aaccgatatc ccttcacagg tttctatatc atcgcccaag tcaacttcag
    28621 gagctgggtt gatattcact tctatttcgg atgtaaaaat ttcatcacat gaattatata
    28681 ctctgcacgt atacattcct gtcatacccg gtgtaatatt cggaacatta tacaccgagt
    28741 cggtagcccc gtcaattatt acatcgttga aataccattg aaaagcaaca aattcgcctg
    28801 ctggatctac cacaaggttc aaggtatccc attcgcaaag gtattgtgtg ccggtattcc
    28861 actgtatgct aggcggggtt ccaacatata attccaccgg atcggaatcg gtttgcccac
    28921 agagattata cattctgcaa aaataggtac cggtatttcc aaactgagcc ggattaaaaa
    28981 taattgtatc gtttgttgcc cacgattctg ctgagccatt gcggtaccat aaataatcaa
    29041 tcggttcagt tccggaagca gccacaatca atgccacgga gtcatcttcg cataccgaca
    29101 tattttctga taatccgaga acttcgggga gcgtatttac cattatctga aatgtttggc
    29161 tttcagcatc gccacacata ttgcttactg tacaaatgta aagtcccgac atctcagctg
    29221 cgatatcggt taataccagt tcttcggaag taccaaagct tactccggtt tcaaagtttg
    29281 tccagtcata ggttagcgga tttgtaccgg aaaccattat cgcaacttca aaatcctggc
    29341 cggcgcatac ttcaatatca tcgggttgca taaggattac cggagggaga tttactgtta
    29401 ctgtagctat ctctgtaagg gttgatccac aatcgttata aacaaaacag gtataatctc
    29461 cggcttgccc ttctgtaatc tcgttgaact gcaatgtttc gctatttgct ccggtgatat
    29521 tgcctccggt aacatttgcc ccattgaaaa gccattggta atagagcggt tctgaaccgg
    29581 atgctttgca atgtaaagta acatactcgc cggcgcaggc caacacatct tcgggctgtt
    29641 gagtaattac aggagactcg aggccggtaa taattgccgt atcggtagaa atccagatgc
    29701 actgattggt aattatacag taataagaat cgataacgcc ctcttcggca ataccattgt
    29761 aggaatcgcc tgtttcaccc gtaataagat cttcgttacg gtaccactga tacgttagcg
    29821 ctatattgct gttggctgtg gctatcaatt gaaaatcttc gccatcgcac aagtcttgtg
    29881 atataggatt tccagttatt accggattcg atagcactga caccgaaaca gcgggtccgt
    29941 aaatagtatc gcagatattg taagcaacag gtacatagaa agtataatcg gggtaaacca
    30001 ctccggcaat aatgagcgta ttttcggttt cgccaggcat ttcaacatta tcggcaagcc
    30061 aggcgtattg tatatttgtt ccggatgctt cagcggtgaa cgacacatcg ttaccttcgc
    30121 aaatttccac acttgaaggg aggcttacaa attgtggggc atcattcacc tgcacggtaa
    30181 tattcccagt agacacagaa ccgcaaacat tggatatttc acaataatat aagcctgagt
    30241 tttcgcttgt aatattttcg atatcaagag tttggccata ttctacaaac cctgaaccct
    30301 gcctgaacca cgaatagctt tcgtaatctg cggttacttc taccgacaac aagagaagtt
    30361 ctccctggca cataattgta tcggatgaat agttatcaat tacaggggca ccgccggcaa
    30421 caagcaaaat tgcatcgtta ctggtggccg acccagacag attagtaaca atacagtaat
    30481 agactccttc atttgattga ttaacacttg atatggcaag gatgggggaa tcggtgccaa
    30541 ccggattgtt ctcgaaatac cattgataat gcaaagggct tgctccggtt gcttctacgc
    30601 taaaacttac tgaattcccg gtgcatttct ctcccgaaag agggtgttgt gttatctgag
    30661 gtacctgaga ataaacacca aaagaaatta taaaaagtaa aaatgtaaaa aaacccgttt
    30721 tgctaattct tttcatcatg aacagtatta ataatagggt gttaaattag caattaattg
    30781 cctaagcagt aaatttagtt tttggtattt tttaaacacg catttgtgaa ttcccacact
    30841 gggatttaac ctaagtttaa cattgatata aatttcattt aatacttgcc caatattttt
    30901 gtatcgaata aaaaccaaat actatgaaaa ttgcctattc catcgttctg attctcttga
    30961 taagcgtagt aacagctttt gccggtaacg acaataccga tgcagataaa acccaaagca
    31021 acggggtaat ttcgggacag gttgttgata aacttacagg cgaaggcttg ggtggtgtta
    31081 aaataaattt cagcaacgga attgaattat ataccgacct aaacggcaac ttcgaattga
    31141 tagttacgtg taacaatagt tttcaattac agaccagtta tatcagttat gaaaccttta
    31201 ccattgccaa cctccacatt aatcccggtg aaaaacaggt actcagggtt gaaatgaaat
    31261 cgattgaaga ttagttttca atgcgttcgg tgatcagtaa ttcattttag cacaaagcat
    31321 gagtaaacca ttattccgaa atacatttat attattgttt tttcaattat cctgcatttt
    31381 ttcattttcg caaggtgtta aaaatatcga cggtttgtat tataccgaca aaaatgaatt
    31441 atacacgggc gattacatcg aattatacga taatggaaac ataaaattaa aaatgcatat
    31501 tgagaacggg cagaaagaag gggaaaccat gttgtacttt cagaatggca gtgtaagcga
    31561 agcgcgtaat taccataaag ggaaaaaaca cggcgattgg gtttgctacg acacaagcgg
    31621 agttaaaatt gctgtcgcac agtatgaaaa caaccagaaa tcgggattat ggctgatatg
    31681 ggatgcaaat ggcatcctgc gat
//
LOCUS       DAOOQP010000021        31277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1669_length_31277_cov_5.470662, whole genome shotgun sequence.
ACCESSION   DAOOQP010000021 DAOOQP010000000
VERSION     DAOOQP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_31277_cov_5.470662"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..511
                     /locus_tag="PLK75_03895"
     CDS             104..511
                     /locus_tag="PLK75_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY38418.1"
                     /translation="MTTARTVYKGSLRTENTHIQSGTVIETDAPVDNKGKGERFSPTD
                     LVATALCDCMLTIMGIAAQEQGFSIDNAKAEVTKIMTDKPPRKIAKVIISLDFSMCII
                     DEKQRKILQAVPRLCPVSLSLHPEVVQEVNFKF"
     gene            516..905
                     /locus_tag="PLK75_03900"
     CDS             516..905
                     /locus_tag="PLK75_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOY38419.1"
                     /translation="MDFADLIGIAGYCDKIAEPTDSASRYGSGLVDVFSTPALVAFVE
                     KTCLLSVNEKLPDGYSTVGVRIELTHEKATPIGKIVFCSSTVCNVEGKKIRFEVVARD
                     EKGIIGQGYHDRYIIEVKSFMAKMQQP"
     gene            1094..2455
                     /locus_tag="PLK75_03905"
     CDS             1094..2455
                     /locus_tag="PLK75_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOY38420.1"
                     /translation="MAKKNVIIIGAAGRDFHNFNMYYRGNANYNVVAFTAEQIPGIEG
                     RPYPPELAGKDLYPKGIPIFKESELPALIKKFKVDDCVFAYSDKPYNYVMSISAIVNA
                     AGANFVLLGPRDTQVKSTKPVIAVGATRTGCGKSQTSRRIIEILVKKGLRVVAVRHPM
                     PYDPDLNKQKVQRFATVEDLKKHNCTIEEMEEYEPHVVSKRNVIYAGVDYEAILREAE
                     KDPDGCDVVLWDGGNNDFPFYVPDLFVGVADPLRPGAEVSYYPGEVVARMADVIVINK
                     IDSASLENTNTVRANLAKINPKATIVDGASYLTVDKPELIRGKRVLVIEDGPTLTHGE
                     MKIGAGTVAAMKFGAAELIDPRPYTVGTLSDTFKSYPNIGTLLPAMGYGDKQVKDLEK
                     TIANTPCDAVVIATPIDLNRIVKIKQPTVKIGYELQEIGFPNLEQILDEFVKKHNLKG
                     KKK"
     gene            complement(2498..3079)
                     /gene="ruvC"
                     /locus_tag="PLK75_03910"
     CDS             complement(2498..3079)
                     /gene="ruvC"
                     /locus_tag="PLK75_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOY38421.1"
                     /translation="MNTTATDRIILGIDPGTNYMGYGVLQITGNNPSLVSLGIVDMHK
                     FENHYLKLKHIYERTIQLIDGYNPDELSIEAPFQGKNVQSMLKLGRAQGAAIAAALSR
                     EIPIFEYAPKKIKSSITGNGNAAKEQVAAMLMKILRFSDMPKHLDATDGLAVALCHFY
                     QSNVITAPSSYKNWEDFLRKNPDRIAKIGKNNS"
     gene            complement(3081..4925)
                     /locus_tag="PLK75_03915"
     CDS             complement(3081..4925)
                     /locus_tag="PLK75_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38422.1"
                     /translation="MRIAGSIPHTAKAIIVLLFVCIVNISPAKAQGYDYDLAMQYYNA
                     GEYDKASVIFQQLYDQYKYRNYLSYYIKCLSELNEFSTAEKYLKKEIKKNSGDPSLYV
                     DLGIVYTAMNDAGKATEQYDKAVKLSLAQKPQTLNTANYFILNRLYEYAETLYLQAAA
                     QQKSNYDYELANLYYYQRDYQKMTEKFLDLLLDYPVYLDNIQNRFQMIMASSTENDLN
                     QLLEASLLQRIQKYPQKTVYAELLIWQYIQTGRNEEAYQQLVSIDKRTNGMGTRLIEF
                     GQMLTQEQEFDLALKCFDYVLQSGQSNPNYNLADIEYLNTLYTKVINSPQRTQEELLT
                     LEAKLKDNIAKTRQKQAFRMIYALASIQAFYLDKMEEASNLLSDVISQQRLSASEIAE
                     CKLLYADILLLSGNYWDAALTYAQVEKANQNNPYGHEARFRKAKLAYYTGQFQWALAQ
                     LDVLKASTSKLIANDAFELSLFITDNSALDTTYTALQMFSRADLLQYKHKDDLALLTL
                     DSVITMFPAHEITDDILFRKAQIFESNGRYNDAIAMYQEVISKYSFDILGDNALYNMA
                     HLYDFTLKDKVKAMDAYQKLITEYPGSIYTVDARKRFRYLRGDKLTEQ"
     gene            complement(4909..5466)
                     /locus_tag="PLK75_03920"
     CDS             complement(4909..5466)
                     /locus_tag="PLK75_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38423.1"
                     /translation="MNKHLIISILLIAIFLAGSSCSNNTQKHAIQKVDSLLDLNRQVY
                     KELSEIGIDSITMMYDTVKKYDNYFATPEVSNIINGNNLVLMYEYGTIDKTFKKLLKE
                     HYAEMLESLEHRRQQLENLKQDAENGILNDSTLLVYLHNEDSLMNFTANDVRGRIKFV
                     EQHKQKYYRYHPEILKLIDSLENSR"
     gene            complement(5459..6736)
                     /gene="serS"
                     /locus_tag="PLK75_03925"
     CDS             complement(5459..6736)
                     /gene="serS"
                     /locus_tag="PLK75_03925"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOY38424.1"
                     /translation="MLTLTDIRENPDRIIRLLQIKNFDASQTIHEIIRLDDTRREIQF
                     KKDKLLNELNESSRVIGNLYKEGRQAEAEAIKQKTAAAKEKSRVFESELSDTESTLQK
                     LLVTLPNAPHESVPTGKTAADNQIIRSGGNIPMLVQKLPHWELITRYDIIDFDLGTKI
                     TGAGFPVYKGKGAKLQRALISFFLDEAEKSGYAEVMVPLMVNEDSGFGTGQLPDKEGQ
                     MYQIGLDNFYMIPTAEVPVTNLYRDVIVPEEKLPIKNVAYSACFRREAGSWGAHVRGL
                     NRLHQFDKVEIVQIQKPENSYQALNEMVEHVERLLVKLELPYRIVKLCGGDISFTSAM
                     TFDFEVFSAAQERWLEVSSVSNFESFQANRLKLRYRCSQSKKNLLAHTLNGSALALPR
                     IVAAILENNQTETGITIPKVLTPYTGFNFIGNE"
     gene            complement(6814..7098)
                     /gene="rpmA"
                     /locus_tag="PLK75_03930"
     CDS             complement(6814..7098)
                     /gene="rpmA"
                     /locus_tag="PLK75_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOY38425.1"
                     /translation="MAHKKGAGSSHNGRESHSKRLGVKIFGGQEAVAGNIIVRQRGTV
                     HHPGKNMGIGKDHTLFALCDGTVKFEKKNNNRSYVSVVPVENQQEAATKE"
     gene            complement(7110..7421)
                     /gene="rplU"
                     /locus_tag="PLK75_03935"
     CDS             complement(7110..7421)
                     /gene="rplU"
                     /locus_tag="PLK75_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOY38426.1"
                     /translation="MYAIVEIAGQQFKVEKGSKLFVHRLSEAEGNNVDFDKVLLIENE
                     NKFTVGRPVVENAKIVAKVVSHVRGDKVLVFKKKNRKGYQVLNGHRQNFSEIEVQEII
                     G"
     gene            complement(7495..9600)
                     /locus_tag="PLK75_03940"
     CDS             complement(7495..9600)
                     /locus_tag="PLK75_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38427.1"
                     /translation="MQQLRIKKQVSLVFRQWNNRSYSVFASLGKLVFIAVLPVTYSLI
                     SAPCKAQSNSTGSYATSPDTLPEVEVVPDDVAIPVFPSRIIEFSQIVFTSSDRSEQAE
                     WLPGFDIRSRGGNGVQGDIQLRGGSPEQTVVMLNGIPMNDMQTGHNTLNLPIPDICIG
                     KITGYSGAESLFFVPGAYNGAVNYSCRNLTENHITMHLTGGSYFLMGYEAMAGFSTGR
                     LNHLIAFSGKQSKGYMFNTDFNTEKAYYMLSGQLHVSGYYRFQAGIASKGFGAQSFYS
                     TRFPNQYESIQTGFVSLQASTGLNFNFAPTMYWKRTHDRFELFRESLYTQDNGYYIWG
                     TDTAKYSENSYTPTAYYQGHNYHLSDRMGLSLTVNRGVEFGTFSANCSYNHDRIVSTV
                     LGEQLEEPKNDPFSTGIELAFGKTRNMANLFAGFNSKSFSGFSFQAGAMGFWFGTGIK
                     PGGGLKLSYSYTQTFSAWLSFNRNQRLPTYTELYYKSVTNIGNSNLHPEDAYNYELGF
                     AVAKNWFHAEEKLFYIDGLNTIDWVRAPDETVYHAMNHTHITTIGNTLTLAFKPLPDT
                     KGIQWLKSFSVSHTYLKKTKFSSELLSAYTLDYLRHKLNANVIIGFGQIVEAEIRISY
                     QTRNGTYTLNNTEVPYQPVFATDVGISSNFGKLTVYVLANNIFNAQTPDFGGIIPSGC
                     WLSAGIKINTGNKSKIVKK"
     gene            complement(9703..10665)
                     /locus_tag="PLK75_03945"
     CDS             complement(9703..10665)
                     /locus_tag="PLK75_03945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY38428.1"
                     /translation="MRLRNIDLLKGVLIILVIVGHILLGSTEQSLPRYIIYCFHIPVF
                     IGLSGFLFNYTKMGQLSLKGFISKYLFRIILPWVIAVLVYKLILNFNVIVQTHDYRIF
                     TGAFKLPYNHLWFIAAYFSWITITWLAGKLHVPLWALLVFAIGLVVTVYFIRKYPPWD
                     AGNPSSQVYSSLIFQIYRPHLYIYFVIGLMLRNNSFSIKIWPVAIVTLSLFVVLMLSF
                     LYRFSFVQWLLYFFNTGLLFILVQYAKKDALPHAKWIEWIGVNSMAVYLWHMVPLIIV
                     SQHIPASPTVLYYTVSFAAVIAFSAIIYFLTKIKPVNKYLFGMY"
     gene            complement(10713..11180)
                     /locus_tag="PLK75_03950"
     CDS             complement(10713..11180)
                     /locus_tag="PLK75_03950"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOY38429.1"
                     /translation="MENKVYEFDATLHREGSQNIAFIEIPFNVEKEFGTKGRVKVDVL
                     FDGYRYRGSIVTMGHPCHILGVNRTIRNAIQKNPGDTVHIVLKQDTEERMVEVPDYIK
                     DALQTDQAIWEKFLRSCYSRRREYIAYIEEAKREETRFSRILKVIEALSKKKM"
     gene            complement(11213..11728)
                     /locus_tag="PLK75_03955"
     CDS             complement(11213..11728)
                     /locus_tag="PLK75_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOY38430.1"
                     /translation="MIEKNKPVQKQIFSTLETLRIRYSYFESPADFVDEDDANFWMKT
                     GAIRCKNLFLRNHKGDRHFLVIAPFYEPVSIYNLEQHFKKGKISFASDQRLQRVLKVV
                     PGAVSVFGLLNDPAHKTEVFLDDRLQQEALLSFLPNYKGAIITLAYTDVIRFLEHTGH
                     RHTVFNLKPCS"
     gene            complement(11730..12854)
                     /gene="meaB"
                     /locus_tag="PLK75_03960"
     CDS             complement(11730..12854)
                     /gene="meaB"
                     /locus_tag="PLK75_03960"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOY38431.1"
                     /translation="MSNKKKFKGDVEECNAENSALKINDGVVQPPQVNPLLKKKPVRK
                     RLLSADEYFNGIISGNRSILGQAITLVESKLPDHQEMAQQLIERCLPVASNSVRIGIT
                     GVPGVGKSTFIEALGTNLVEKGNKIAVLAVDPSSERSKGSILGDKTRMENLSVHPNAF
                     IRPSPSAGTLGGVARKTRETIVLCEAAGYNIIFIETVGVGQSETVVHSMVDFFLLLML
                     AGAGDELQGIKRGIIEMADALTINKADGNNVDKANMAKAQYKSALQLYPVSESGWKPR
                     VLTCSAINNAGIPEIWQMITEYMSFTRNNSYFENNRIKQALYWMTQSIDDNLLSHFYQ
                     NEAIQQLIKAYQQKVLANEISSFVAAKHLIDHYFSHIVAK"
     gene            complement(12857..13822)
                     /gene="rfaD"
                     /locus_tag="PLK75_03965"
     CDS             complement(12857..13822)
                     /gene="rfaD"
                     /locus_tag="PLK75_03965"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOY38432.1"
                     /translation="MIVITGAAGFIASNLVSRLNNEGYFDLVLVDDFSRNDKLANYHA
                     KKYTQLVERHLFSEWLRQNHRLVEFVFHLGARTNTTEFDTSVFDRLNLNYSKEVFLLC
                     VEFGLPLIYASSAACYGMGELGYSDDHSLVEKLHPLNPYGESKLNFDRWVLAQERKPY
                     FWAGIRFFNVYGPNEYHKVRMASVVMHAYRQIHETGRMRLFRSHRSDVADGEQQRDFI
                     YVKDAVDVLFFFMLLRKHSGIYNLGTGTASSFNQLAAACFDALKKPLSVEYFDTPADI
                     RDKYQYYTCADISKLRSVGYHTVFTNLNSGVGQYIGNYLSTNSYA"
     gene            14066..17728
                     /locus_tag="PLK75_03970"
     CDS             14066..17728
                     /locus_tag="PLK75_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY38433.1"
                     /translation="MKKEPKRLIAVSILFVLALASIITGSLLQSRSQSLRNIAKELSS
                     VVVGANQNAEQIFHLIEDAGSSEKRDSVVFYSFTELYRDQRAGVFVYDTTGLRCWTTN
                     RIPAPLAADSIYVNNALVRLSDGWYFTAQKLSDDYRILYFLLIKHDYSYENEYLDKDF
                     NRFLSVPDNVLFSIDSIENSEPVLSGKTPLFYVTIDDIAATTGGAFLLTQGAGFMLLI
                     ALLALLWQISAILVRKNISVLFVALGFAMVLTGLRILMLRFQIPSALYALPVFSPVLY
                     AESFWYPSLGDLGISLLFFCVWTLFFMRYFTIRPFAENRYSKGIKPLAGVSVAIVAFS
                     AHLFISARISSLILNSSIELNLVHILRFSAHSVLAIGIVILLLLCTVILFDKIIRELR
                     SIFHPMIFIISLALVSTLWYLFAGAETEAFVLVFLYLGIMSIILLVRIRYKIYNWYSF
                     LALLIVASLYISEHISWVSDAKEIEARKVLANNLMNERDVGAEFFITTIDGSIRNDTQ
                     IEKLIHNDNTDSLYTYFSQKYIKGYLNKYDLQVSVCKFNDSILIQPDNKLENCFTFFG
                     SLLSENGIPIPGTNFYYLENHTGRISYFGYYTYPDDGNSLLQLFVELNIKVFSEGLGY
                     PELLLDKKFAVKKTLKEYSYAKYNDGALILKKGDYEYPMKVKKRSGKDDYRVIVEDSY
                     SHLYYYSGDDNLVVLSIPEAGFKGHFVSFSYLFFVVFLLFSVFRLFVYFRDRKFIDTN
                     SLKNRIQFSIVSIILISILVIGFISVYFIVDGYTEKHNQMLRDKLQSVLVEIEHKFKS
                     EAELDSAQNDYVTSLLIKFSNVFYTDINIFGTDGKLITSSRMELFEKGLQGTFMHPKA
                     YRQLHGEKNGLVIINERIGTINYLSAYIPFRNADNQTIAYINLPYFSKQDELNDEITG
                     FIIAFTNVFLILIILSIAVGIIIANQLTKPLSFLQESIRAIDISKKTEKIDYLRNDEL
                     GSLIREYNKKIDELAESAGKLAKSERESAWREMAKQIAHEIKNPLTPIKLSIQYLEHT
                     YKYNDKSWEETLKKVAKTIVEQIDTLSAIATEFSNFAQMPQAQKQPTDLIEKIKSSLT
                     LFQQSEPDIEFRFIDQSEGVSMIVADPEQLTRIFNNLIKNSIQAIPDTRKGNIVLSLQ
                     HANHYFLISISDNGTGIDEAMKSKIFQPSFTTKTSGMGLGLSMVKNITDQMGGEIWYE
                     TVADEGTTFFIRLPEG"
     gene            complement(17988..18881)
                     /locus_tag="PLK75_03975"
     CDS             complement(17988..18881)
                     /locus_tag="PLK75_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY38434.1"
                     /translation="MKKFIMTLLAIYIVFVAIGQTGNIELNILGNDDDDASNSSLSVE
                     LWLNTQLVTTKTSDDCLFFENIPGDVYRIVITSAGEELLTYNNVLVEENSTTYLDINL
                     PVAEIINDSVPEEFPAYIEFVLPFRFSFPVLEDSNYPIKREIAFGYEYNIVMPVAKHF
                     AIINTVGTGFYFYRVKDNMDVTPALNDKEGFFKWTLSEGLLFRVSSENLKTAPVFGWY
                     LDLGAVYHFPLLFTYKYKLDHTRTATNKIHKYTDVSLMAKFGYAPFGLFVEYNPFSYV
                     KDPYPQMPKLRFGVSIQFPMN"
     gene            18999..19784
                     /locus_tag="PLK75_03980"
     CDS             18999..19784
                     /locus_tag="PLK75_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38435.1"
                     /translation="MKKLIYGILLIGFVLFLNSCSVQKQVINDPEEVIQLRMSNLDDG
                     TNGSGVAYNSDLKLYYTVIAGNAEFPLEVFEENGENVYTASAGVDCRGLWYNKSTKTL
                     EANVYQGGLYKIIISEEGIPTGQIAAVFDGGEQPYSNSCAEYNFKSGKLLYYYSGTVY
                     TANRKNGKIESKIKLELPCNIEDINTTSIIYTGYKGMEIGILNCGQWKLYLFNLKDGS
                     LKATVNIPDYMTVDCAFMFDFANDYAFFYNVNDRIWSGIKIFK"
     gene            complement(19986..20267)
                     /locus_tag="PLK75_03985"
     CDS             complement(19986..20267)
                     /locus_tag="PLK75_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOY38436.1"
                     /translation="MNIFVAKLSPSTTSEDLKNLFAAYGEVTSAKVIIDKNTGFSKRY
                     GFVEMSNENEANEAISKLNETEFDKSVIVVKIAQPREETGQRHTGYNRR"
     gene            complement(20468..22201)
                     /locus_tag="PLK75_03990"
     CDS             complement(20468..22201)
                     /locus_tag="PLK75_03990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY38437.1"
                     /translation="MKTFEELGIQPDIIKGIKDCGFEMPLPVQEEVIPLLLHETRDVL
                     ALAQTGTGKTAAFGLPIIQNTKIEIYKPQTLILSPTRELCLQITDDLKDYAKYIDGIK
                     ILAVYGGSSIETQIKALHQGVHIVVATPGRLIDLIERKVVNLKEVKRIILDEADEMLN
                     MGFLESIDKILENLPKGINSLLFSATMPPEIARIANKYMNNPVEITIGVRNTGLENIR
                     HFNYTVQAKDKYLALKRIVDYYPDIYGIIFCRTRRETQEVADKLMADGYNAESLHGDL
                     SQAQRDTVMQKFRIRNVRILVATDVAARGIDVNNLTHVINYNLPDDKDAYTHRSGRTG
                     RVGKAGISVVLTNLREKSQIRLIEKNSGIQFIQAKVPNGKEVCEKQLYHLIDRIEKVE
                     IEHAEIEPYLPAVYRKLEWLDKEELIKRLVSVEFNRFLLYYKNTKDLNLNEEKYQNSG
                     ANQGAEKRVKKDIPDNRDFQRKAYTKMFIGLGRNDGLYPNLLIDLLRKEVNGVKLNIG
                     KIDLFNKYSIFELDSNVVEMVIEAFSDIYLEDRKIIVKIDTEKPSLKPGDFNKGEARS
                     DTRFKPKYKKR"
     gene            complement(22425..22916)
                     /locus_tag="PLK75_03995"
     CDS             complement(22425..22916)
                     /locus_tag="PLK75_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38438.1"
                     /translation="MKKLVFLLVLVVAGFATANAQVNPHAIGVRFGGGTYYGAEVSYQ
                     HGLGESHRMELDLGWNGGLYHTRVFFSGIYHWHWNIVEGLNWYIGPGAFVGMYLDEVK
                     LGVGVGGQVGIEYDFNTLDVPILVSLDSRPMFHFLPWYDRDDIHYMSPFDPSVALSVR
                     YTF"
     gene            23122..25701
                     /locus_tag="PLK75_04000"
     CDS             23122..25701
                     /locus_tag="PLK75_04000"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOY38439.1"
                     /translation="MSKYFKTAILPHLIAGIIFLIISIAYFSPAFLEGDRLNMHDWKV
                     WQASSKEKAEFQNQTGEITNWTNSMFSGMPTYLISTPKEQNVFTYVQKIMGFGFGEPV
                     TFLFYYLLGFYILLQVFKVDVRLAIAGAIGFGFSSYFFVIITAGHLTKAAAIGFIAPI
                     IAGVYLAYDRNMWKGMLLMTFALALQILTNHLQIVYYTMMIILGFGIAWFIGAIMEKT
                     LLTFLKTTGVLIAGAALAVGVNATNLITTQEYAPYSMRGQSELTNAQHLRTTGLNRDY
                     ITGWSYGKTETFTLLIPNAKGGASYAPLSDESETYNVLAKYYGEDVAGDIIKQMPVYF
                     GPQPSTSGPVYIGAIVFFLFILSLFVVKGKIKWWLLSVTILSILLSWGRHFMWFTNLM
                     LDYFPAYSKFRTVSMTLVIAEFTMPLLAFLGLREIFSGKVEPKKLMNYFYLSIGVTAM
                     ILLVFIAYPKATGNTSEHEEKFVSQQLLAGIPDDPKYDQVKQDIMQEFTTAIQTDRLS
                     LIRNDAARSLAFAVLAALVVFVVMKRKMKAVYAAIILAALILVDMWTINKRYLNMDNF
                     VSERTFEVPYTASRADEIILSDKNSDFRVCNLSMGLSGVFADASTSYFHKSIGGYHGA
                     KMLRYQELMDSVMAGELYMAAYMINVAGQNKMSFAEMQMLFDSSGNFPVLNMLNTKYI
                     IYSPEGAPIRINSALGNAWFVDSYSLAEDANQEILMMRKLNTASEAVIDKRFESELKG
                     LKITPDSTAAIRLVEYKPNYLKYESQSAADGLVVFSEIYYSKGWQATIDGKPKQHFRA
                     NYVLRSIVVPKGKHTIEFRFDPQSYKTGNLISYICSGIIFLMIIASAVLFYIKRRKGT
                     KTT"
     gene            complement(25784..27289)
                     /locus_tag="PLK75_04005"
     CDS             complement(25784..27289)
                     /locus_tag="PLK75_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOY38440.1"
                     /translation="MKNSIIWVMFCLSVLFSLLFYRQAIGVNLLIFEGLALPLMLWNY
                     SPFKKTGIFWFMFVGTVLSLFSVFFYHTAWSFTVHLIFAFMFAAFMSFPTMRSLLHLW
                     VESIAHIITSQIGFFVFLARKPKNRISWGKFGRVLKWGLIPLAIIILFIFLYRIANPI
                     FNNQIIRLIDAINYWLSFITWGWFWMAVFGFFLCTIFFVKFSHSLMHNNDIAAKDTMT
                     RLPRRRGRSFRFSSLINEYKSAIFLLVALNALILYLNVIDIIWVWFGFEWNGDYLKQF
                     VHSGTWILVVSILISMFIVIYYFRRNLNFYSKNKWLKVLATLWMVQNFIMTISVVIRN
                     LWYINYFALAYKRIAVLFFLLLVIIGIISVIVKIYHRKTGYWLVRVNSIALFSVLLIS
                     VFINWDLMIAKYNFSHYERSFVHFNFLEKMSTNALPELDKTPEELQVIHETQKHKMPF
                     DVEDRVKYMDYVTYYSAIQSKKANFCKKYESRHWLSWNYADYRTYKALKSK"
     gene            27495..28163
                     /locus_tag="PLK75_04010"
     CDS             27495..28163
                     /locus_tag="PLK75_04010"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOY38441.1"
                     /translation="MKRINLISIFILVAVTVVAQHVQQPLPYAYNALEPWIDAQTMEI
                     HYSKHHKAYIDNLNKAVQGTSYSGYDLTKLQFNITGETPAVIRNNGGGHWNHEFFWNI
                     LTTPNEDKIPEDLLGALISSFGSFDNFKAEFKKNAMGRFGSGWAWLIVKNGKLLITTT
                     PNQDNPLMPVAEIQGVPILGIDVWEHAYYLKYQNKRGDYVDAFFNVVNWNKVAELYKQ
                     NYKK"
     gene            complement(28183..29118)
                     /locus_tag="PLK75_04015"
     CDS             complement(28183..29118)
                     /locus_tag="PLK75_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818591.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOY38442.1"
                     /translation="MQVERQPKPWNDYAAYLRKRFGSRVQKISVDAGFTCPNRNGTLS
                     ETGCIYCNNETFSPFYCNPKKTITHQLNEGIAFFSKKYRTQNYLAYFQSYTNTYAEIE
                     HCISLYQEALSVDNVIGLVIATRPDCVSNELLKEISLIAKDKYTVIEFGVESTNNQTL
                     RRINRFHTYEQSVEAIARSNENGIYTGIHLILGLPDEDRDTMILHAKNISDLPVHNLK
                     IHQLQIIKGTVAESMFREKPEQFTLFGIDEYVALVSEFLCYVRPGIMPDRFSSESPQE
                     MLIAPCWGGLKNYELIHRVEKFMISKGWTQGMHTH"
     gene            complement(29121..29843)
                     /locus_tag="PLK75_04020"
     CDS             complement(29121..29843)
                     /locus_tag="PLK75_04020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY38443.1"
                     /translation="MNEEIDIRFERKRLILAATIPAIILLICWLIKLFEISSGISLSN
                     YGVLPRNLHSLFGIILMPLLHGDYAHLAANSVAFLVLGTGVFYFYRDIAVKVLLFSWL
                     STGIITWVIGRESYHIGASGLVYAFAGFIFLSGVLRNHFRLMAISLLVVFLYGSMIWG
                     IFPIEPNISWEGHLSGLFTGFVLALIYRSKGPQRPRYSWEEEDDEDDVNESNNEKGAP
                     SNTSATHRISPEYDYKPNENKN"
     gene            29923..31074
                     /locus_tag="PLK75_04025"
     CDS             29923..31074
                     /locus_tag="PLK75_04025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY38444.1"
                     /translation="MKLLVLNYEYPPLGGGAGVITQNIAQGLVKLGNSVCVVTTGFKD
                     LPPDSLENDVRVIRLPSKRRFVFQSNPVEMWSWMKHANTFLRKHLKTEKYDLCFANFS
                     IPGGAVALNMKREFGLPYTVISHGHDIPWFFPRQMVWYHLILYHKIREIVLNSEANFV
                     QSNEMKRNIDLFTGNLFASKNHLIYNGWNSSVFTADYSKRKSEFILLFVGRLVPQKDP
                     FTFLKAVKIAATTIDLQVHILGDGPLRAKMEKWVTKNHLSEKVKFFGWVDKPEMLYQY
                     QSAAVTVLPSLSEGMSIATLEALASGQYVIATRVSNNESLIVENINGNLLNMKDHTML
                     ADLIVKFYHEKFLKYYRIPPEELEKYRSLYEWDGIVEQYNNIFARIIGS"
BASE COUNT         9087 a         6140 c         6444 g         9606 t
ORIGIN      
        1 agaattactg ggttataaac cagtatcatt gctaatgcat attgaaatgt gagaaatagt
       61 tagcattaaa aacaatgctt aggtatcaat taaaaaagat gttatgacaa cagccagaac
      121 agtgtataaa ggatcgctga gaacagaaaa tacgcatatt caatcaggta ctgtgattga
      181 gaccgatgca cctgtcgaca acaaaggtaa gggtgagcgt ttttcgccaa ccgatttagt
      241 ggcaactgcg ctatgcgact gtatgctcac tattatggga attgctgcgc aggagcaggg
      301 tttttcgatt gacaatgcca aagctgaagt aactaaaatc atgacagata agcctccaag
      361 gaaaattgca aaagtgatta tctccctcga tttttcgatg tgcattattg acgaaaaaca
      421 gcgtaagatt ttgcaggcag ttccccgctt atgtcccgta tcgctaagcc tgcatcctga
      481 ggtagttcag gaagtgaatt ttaaattcta atattatgga ttttgctgat ttaattggaa
      541 ttgccgggta ttgtgataaa atagcagaac ctaccgattc agcatcgcgg tatggctcag
      601 gtctggttga tgtgttttca acccctgctc tggttgcttt tgtcgaaaaa acatgtttgc
      661 tttcggtgaa tgaaaaattg cctgatggat acagtactgt gggggtgcga atagaactga
      721 cacatgaaaa agctactccc attgggaaaa tcgtattttg cagctcaaca gtctgtaatg
      781 tggaagggaa aaaaatcagg tttgaagttg ttgcccgcga tgaaaaagga attatcgggc
      841 aaggttatca cgacaggtat attatcgagg tcaaaagttt tatggccaaa atgcaacagc
      901 cttaatacta tgtttcttaa catatcgttc tgaaaccttg cattttcaga caattcatga
      961 tgattctatt atatcattca gaattataaa cgattttgtg ttttttcatg ttctaatgaa
     1021 atattaaaat ttatctttgc ttttcgtagt taactaatta atgctattat aaaactaaat
     1081 aaatcaacag gttatggcaa agaaaaacgt aattatcatt ggagctgccg gaagagattt
     1141 ccacaacttc aacatgtatt acagaggtaa tgccaactat aacgtagtgg cttttactgc
     1201 tgaacagatt cccggtatcg aaggaagacc ctatccgccc gagttggcag gcaaggatct
     1261 atatccgaaa ggcattccca ttttcaaaga atcggaattg cctgcattaa ttaaaaaatt
     1321 taaggttgac gactgtgttt ttgcatacag cgataaaccc tataactatg taatgagtat
     1381 aagtgcaatt gttaatgcag ccggagccaa ttttgtgctg ttaggcccca gagataccca
     1441 ggtaaaatcg acaaaaccgg ttattgccgt gggtgctaca cgtacaggat gcggaaaatc
     1501 gcagacatcg agaaggatta tcgaaatttt ggtaaaaaag ggtttacgcg ttgtggcagt
     1561 tcgccaccct atgccttacg atcccgatct gaacaaacaa aaagttcagc gttttgcaac
     1621 ggttgaagac ctcaagaaac acaactgtac cattgaagaa atggaagaat acgaacccca
     1681 tgtggtttcg aaacgcaatg tgatttatgc cggcgtcgac tatgaagcca ttcttcgcga
     1741 agccgaaaaa gatcccgatg gatgcgatgt tgtgctttgg gatgggggta acaacgactt
     1801 tccgttctat gttcccgatt tgtttgtagg cgttgccgac ccattgcgtc cgggtgctga
     1861 agtgagctat tatcccggcg aagtggttgc ccgcatggcc gatgttattg taatcaacaa
     1921 aattgactca gcttcactcg aaaacacaaa taccgtaagg gctaatctgg caaaaatcaa
     1981 ccccaaagcc accatcgttg atggagcttc gtatctaacg gttgataaac ccgaacttat
     2041 acgtggtaaa agagtgctcg tgatcgaaga cggacccaca cttacccacg gtgaaatgaa
     2101 aattggagct ggtacagttg ctgccatgaa atttggcgct gcagaactga tagacccacg
     2161 tccttatacc gttggtacac tcagcgatac ctttaaatct tatcccaata tcggcacctt
     2221 gcttccagct atgggttacg gcgacaaaca agtgaaagac cttgaaaaaa ctatcgccaa
     2281 cactccttgc gatgcagtag ttattgcaac tcccatcgat ttaaacagga tcgttaaaat
     2341 taagcaaccc actgtgaaaa tcggttacga actgcaggaa atcgggttcc cgaatcttga
     2401 gcaaatcctc gacgaatttg tgaaaaaaca caatttgaag ggaaagaaaa aataaatgga
     2461 aaaaccataa attaaggctt gcttcggcag gcctttttta tgaattattt ttaccgattt
     2521 tagcaattct atccggattt ttccgaagga agtcttccca atttttatag gatgagggtg
     2581 ctgtgataac gttcgactga taaaaatggc ataatgccac ggccagaccg tcggtagcgt
     2641 cgaggtgctt gggcatatcg ctgaaacgga gaattttcat aagcattgct gccacctgtt
     2701 cctttgcggc atttccatta cctgtaattg aactttttat tttttttggt gcatattcaa
     2761 aaatagggat ttcgcgactg agagcagcgg caatagcagc accctgtgcc cgacccaatt
     2821 ttaacatcga ctgcacattc ttaccctgaa aaggtgcttc aatcgacagc tcgtcggggt
     2881 tatacccgtc aatcagttga atggtccttt cgtagatgtg tttcagcttg aggtagtgat
     2941 tttcgaattt atgcatatcg acaatcccca gacttaccag cgaaggattg ttcccggtaa
     3001 tttgcaacac accgtatccc atataattgg tgccggggtc aatgcccaga ataatccggt
     3061 cggttgcagt cgtgttcata ttattgctcg gtaagtttat cgcccctgag gtaacggaat
     3121 cgttttcgtg catctaccgt atatatgctt cccgggtatt cggttatcag tttctggtag
     3181 gcatccatgg cttttacctt gtctttcagt gtgaaatcgt agaggtgcgc catgttatac
     3241 agcgcattgt cgccaagtat gtcgaaggag tatttgctga ttacctcctg atacatagca
     3301 atggcgtcgt tgtacctgcc attcgattca aatatctgag ctttgcgaaa caaaatatca
     3361 tctgtaattt catgagccgg aaacatagtt atcacactgt cgagggtgag cagtgccaaa
     3421 tcatctttgt gcttgtactg caacaagtct gcacgactga acatttgcag ggctgtgtag
     3481 gtggtatcaa gtgcagaatt atcggtaata aaaagcgaca attcgaatgc atcgttggca
     3541 atgagcttac tcgtacttgc tttcagcaca tcgagctgcg caagagccca ctgaaactga
     3601 ccggtgtaat aagccagctt tgctttacga aaccgtgcct cgtgaccgta aggattgttt
     3661 tgatttgctt tctcaacctg ggcataggtg agagcagcat cccaataatt tcccgaaaga
     3721 agcagaatat cagcgtagag tagtttgcac tcagcaatct cggaagcact gagtcgctgc
     3781 tggctgatga catcactgag taaattcgat gcttcttcca ttttatcaag gtaaaaagcc
     3841 tgaatgctgg ccagagcata aatcatccga aaggcttgct tttgtctggt ttttgcaatg
     3901 ttgtccttga gttttgcttc aagagtcagc aattcttcct gtgttcgttg cggtgaattg
     3961 attacttttg tgtacagtgt gtttagatat tcgatatcag ccagattgta gtttgggttc
     4021 gattggcctg attgcaaaac ataatcgaaa catttgagcg ctagatcaaa ctcctgttcc
     4081 tgcgtaagca tctgaccgaa ttcaatgagc cttgtcccca tgccgttggt gcgtttgtcg
     4141 atgcttacca attgctgata ggcttcttcg tttcgcccgg tttgaatata ttgccagatg
     4201 agtaactcgg catatactgt tttttgcgga tatttctgta ttcgctgcaa gaggctggcc
     4261 tcgagcaact ggttgaggtc attttcggta ctgcttgcca tgatcatctg aaatcggttc
     4321 tgaatattgt cgagataaac cggatagtcg agcagcaaat ccagaaactt ttcagtcatt
     4381 ttttggtagt cgcgctgata ataatataga tttgctagtt cgtaatcgta gttgcttttt
     4441 tgttgtgcag ccgcctgaag gtaaagtgtt tcggcatatt catacaagcg gtttaaaatg
     4501 aagtagttgg cagtattaag ggtctgtggt ttttgtgcaa gcgatagttt tactgcttta
     4561 tcgtattgct ctgttgcttt gccggcatcg ttcattgcgg tatatacaat tccaaggtcg
     4621 acatataaac ttggatcgcc ggaattcttc ttaatttctt ttttcagata cttctcagca
     4681 gtactgaatt cgttcagttc cgacaagcac tttatatagt agctgaggta gttccggtat
     4741 ttgtactgat cgtagagttg ctgaaaaata acagaagcct tatcgtattc acccgcattg
     4801 taatactgca ttgcaaggtc gtaatcgtac ccctgagcct ttgccgggct gatattgaca
     4861 atacatacaa acagcaaaac tataattgct ttcgctgtat gtggtatgct acctgctatt
     4921 ctcaaggctg tcgattagtt ttagtatttc agggtgataa cggtaatatt tctgtttatg
     4981 ttgttcaaca aacttaattc ttccacgcac gtcgttagcg gtgaaattca tgagcgaatc
     5041 ttcgttgtgc aggtaaacca acaatgtgct gtcgttaagg ataccatttt cggcatcctg
     5101 cttcagattt tcgagttgtt gccgccggtg ttcaaggctt tcgagcatct cggcataatg
     5161 ctctttaagt aattttttaa aggttttgtc gattgtgcca tattcgtaca tcagaaccaa
     5221 attgtttccg tttatgatgt tgctgacttc gggtgttgca aaataattat catatttttt
     5281 cacggtatca tacatcattg taatactgtc gatgccgatt tccgacaatt ctttgtacac
     5341 ctgacggttt aaatcgagca aagagtcgac tttctgaatg gcgtgttttt gtgtattatt
     5401 gctgcatgaa ctaccggcca aaaaaatagc aataagcaat atggagatta ttaaatgttt
     5461 attcattgcc aataaaatta aatcctgtgt aaggtgtcaa aaccttggga attgttatac
     5521 ccgtttcggt ctgattgttt tcgagaatgg ctgctacaat tcggggaagg gcaagtgcac
     5581 tgccattgag tgtgtgagcc agcaggtttt ttttgctttg gctgcaacga tatctgagtt
     5641 tcagacgatt tgcctgaaag ctttcgaaat ttgagacgga acttacttca agccaacgtt
     5701 cctgtgctgc actgaacact tcaaagtcga aagtcatagc cgatgtaaaa ctgatgtcac
     5761 caccgcagag tttcacaata cggtagggta gttcgagttt taccagaagt ctttcaacgt
     5821 gttcaaccat ttcgttcagt gcctgatacg aattctccgg cttttgtatc tgcacaattt
     5881 cgaccttgtc gaactgatgc agacggttta atccgcgcac atgggcaccc cagcttccgg
     5941 cttcacggcg gaaacaagct gagtatgcga cattcttaat cggcagtttt tcttcaggga
     6001 caataacatc gcggtacaga ttggtaacgg gcacttctgc ggtaggaatc atataaaaat
     6061 tatcgaggcc tatttgatac atctgccctt ccttgtcggg aagctgacct gtgccaaaac
     6121 cactgtcctc gtttaccatg agcggaacca taacttcggc atagcctgat ttttcggctt
     6181 catcgagaaa aaagctgatc agagcacgtt gcagtttggc acctttccct ttatatacag
     6241 gaaatccggc tccggtaatt ttcgtgccca aatcgaaatc gataatgtcg tatcgggtaa
     6301 tcagttccca gtgtggaagc ttttgtacta acataggaat gttacctccg ctgcggataa
     6361 tctgattatc ggctgcagtt tttccagtgg gtactgattc gtgcggtgcg ttgggcaagg
     6421 taacaagcaa cttttgaagg gttgattccg tgtcggaaag ttccgattca aaaactcgcg
     6481 acttttcttt tgctgctgcg gttttttgct tgattgcctc tgcttctgcc tgcctccctt
     6541 ctttatataa atttccgata acacgcgaac tttcgttcag ttcgttcagt aatttatctt
     6601 ttttaaattg aatttcacgt ctggtatcat caagtctgat gatttcatga atggtttgtg
     6661 aggcgtcaaa gtttttaatt tgtaacagcc tgattattct gtcggggttt tcgcgtatat
     6721 cggtgagagt taacataaaa gtaaaattaa aagaaatcct gttttttcag ctaaattaga
     6781 aaaaacagga tgcttaatga aaaaaatatg aaattattct tttgtggctg cttcttgttg
     6841 gttttcaaca ggaaccaccg atacataaga acggttgtta ttcttttttt cgaatttaac
     6901 agttccgtcg cacagggcaa acaacgtatg gtcttttcct atgcccatgt ttttacccgg
     6961 atggtgcacc gtgccgcgtt gacgaacaat gatatttcct gcaacagctt cctgacctcc
     7021 gaaaatttta acgcccagcc gtttactgtg cgattctctg ccgttgtgtg aacttccggc
     7081 tccttttttg tgtgccataa ctgaattatt taaccaatga tttcctgaac ttcgatttca
     7141 ctaaaattct gacgatgtcc gttgagaacc tgataacctt tgcggttttt tttcttgaaa
     7201 accaaaactt tatcgccacg aacatgagaa actactttgg caacgatctt agcattttca
     7261 actaccggtc ttcctacggt aaacttgttt tcgttttcaa tcaacagaac tttgtcaaag
     7321 tcaacattat ttccttcggc ttcagaaagg cggtgaacaa ataatttgct ccctttttca
     7381 accttaaact gttgccctgc tatttcaaca attgcgtaca tctttattac tttatcaaaa
     7441 atttatactc acttttggga gggcaaagat agaaaaagaa taataactat aaaattattt
     7501 tttaacaatt ttgcttttat tgcccgtatt tattttaatt cctgcactta accaacaacc
     7561 agatggaata attcccccga aatcaggtgt ctgtgcattg aatatgttgt ttgccagaac
     7621 atacactgtt aattttccga aattggaaga tattcctacg tctgttgcaa atactggttg
     7681 ataaggtact tccgtgttgt ttaaggtgta ggtaccattc cttgtctggt acgatattct
     7741 gatttcggct tccacaatct gaccgaatcc gattataaca ttggcattga gtttatgtcg
     7801 cagataatcg agggtatatg ccgaaagcaa ttcactggaa aatttagtct ttttcagata
     7861 tgtatggcta accgaaaatg attttagcca ctgaatcccc tttgtatccg gcagaggttt
     7921 gaatgcaagc gtaagggtat taccaattgt ggtgatgtgg gtgtgattca tggcatgata
     7981 cactgtttca tcaggagctc ttacccagtc gatagtgttc agtccatcga tataaaataa
     8041 tttctcttca gcatgaaacc aattttttgc aacagcaaac cccagctcat aattataggc
     8101 atcttcggga tgcaggttgc tgttgccgat atttgttacg cttttgtaat acagttccgt
     8161 gtaggttgga aggcgttggt ttcggttgaa cgaaagccat gctgaaaaag tttgcgtata
     8221 agagtaactc agtttcaatc ctccaccggg ttttattcct gttccgaacc agaatcccat
     8281 ggctcctgcc tgaaagctga atcccgaaaa gcttttacta ttaaaaccag caaacaggtt
     8341 tgccatattg cgtgttttac caaaagccag ctcaattccg gtcgaaaacg ggtcgttttt
     8401 tggctcctca agctgctctc cgagcacagt actaacaatt cgatcgtgat tataactgca
     8461 gtttgcactg aaagtcccaa actcaacacc cctgttaacg gtaagtgaaa gccccatccg
     8521 atcgcttaga tgataattgt gcccctgata gtaagccgta ggtgtatagc tgttttctga
     8581 atattttgct gtgtcggtgc cccagatata atacccgttg tcttgagtat agaggctttc
     8641 tctgaaaagc tcgaatctgt cgtgggtacg tttccagtac atggtcggag caaaattgaa
     8701 attcaagcct gtacttgcct gtaacgatac aaaaccggtt tgtatcgact cgtactggtt
     8761 ggggaaacgg gtggagtaaa aactttgagc accaaaacct ttcgaagcaa ttcctgcttg
     8821 aaacctgtag tagcctgaaa cgtgcagttg cccagaaagc atataatacg ctttttcggt
     8881 attgaaatcg gtattaaaca tatagccctt gctttgtttt cccgaaaacg cgatcaaatg
     8941 attcaacctg ccagttgaaa atcctgccat ggcttcgtaa cccataagaa agtatgagcc
     9001 tcctgtaagg tgcatggtaa tatgattctc tgtcaggttt ctgcaactat agtttacagc
     9061 cccgttgtag gctccgggta caaaaaaaag cgactcggca cccgaataac cggtgatttt
     9121 accaatacag atatccggta tcggcaggtt tagggtatta tgcccggttt gcatatcgtt
     9181 cattggtatt ccgttgagca ttacgacggt ttgttccggc gacccgcccc taagctggat
     9241 atcgccctga acgccattac ctcctctcga tcgtatatca aaaccaggaa gccattccgc
     9301 ctgctccgac cggtcgctgc ttgtaaaaac aatctggctg aattcgatga tacgggaagg
     9361 aaacacagga atggctacgt cgtcaggtac aacctcaacc tcgggtaagg tatcagggct
     9421 cgtagcataa gaccccgtgc tattcgattg tgccttacag ggagcactta tcagggaata
     9481 tgtaaccgga agtacagcaa taaaaacaag ctttccaagt gaagcaaaaa ctgaataact
     9541 tctgttattc cattgcctga aaaccagact tacctgcttt ttaatccgta gctgttgcat
     9601 gctgcaaaaa tagaagaatt ttatattgat ttgttgattg ctttatgtga agattaacag
     9661 atttgttgat tatctgatta ataacttgtt agaagttggt aattagtaca tgccgaacag
     9721 atatttgtta accggtttta ttttggtaag aaaatatatt attgcggaaa atgcaattac
     9781 ggctgcgaag cttactgtat agtacagaac cgtgggggat gctggtatgt gctgactgac
     9841 aataatcagg ggaaccatgt gccaaaggta tacagccatc gaatttacgc caatccattc
     9901 aatccacttt gcatggggaa gtgcatcttt ttttgcatac tgcacaagta taaatagtaa
     9961 tccggtattg aaaaagtaca atagccattg aacaaaactg aagcgataca ggaaactaag
    10021 cattaacacg acaaatagcg aaagtgttac gattgccaca ggccatattt ttatggaaaa
    10081 agaattgttg cgcagcatta aaccgattac aaaataaata tacagatgag gacggtagat
    10141 ttgaaaaatc aggctggaat atacctgcga agaagggttg cctgcgtccc atggtgggta
    10201 ttttcgaata aaataaaccg ttacaacaag cccaattgca aataccaaca atgcccataa
    10261 tggaacgtgc aattttcctg ccagccatgt aatggtaatc cagctgaaat atgctgcgat
    10321 aaaccacaga tgattgtagg gtagcttgaa tgctccagta aaaatacggt aatcgtgggt
    10381 ttgcacgatc acattaaaat ttaaaatcag cttatacaca agcacagcaa ttacccacgg
    10441 cagaattatg cggaagagat atttcgatat aaatcctttt agcgagagtt gtcccatttt
    10501 tgtgtaattg aacaaaaatc cgctaagtcc gataaaaacc ggtatgtgaa agcaataaat
    10561 gatatacctt ggtaatgatt gctctgttga tcccagcaaa atatgcccga caatcaccag
    10621 aattatcagg actcctttta ataaatcgat gttacgcagg cgcatgatgt agaatttact
    10681 gttttacaaa tatagtatcg caactatctc tgctacatct tttttttcga taaggcttca
    10741 atgaccttca aaatacggct gaaccgtgtt tcttcccttt tggcttcttc gatgtaggca
    10801 atatactctc ttcttcgact gtagcatgac cggaggaatt tctcccatat agcctggtca
    10861 gtctgaagag catcttttat atagtcaggg acctccacca ttctttcttc ggtatcttgc
    10921 ttaagaacga tatgtactgt gtcgcccggg tttttctgaa tagcatttcg tatagttcgg
    10981 tttacgccaa gtatatggca gggatgcccc atggtaacaa tagaaccgcg ataccgatag
    11041 ccgtcgaata aaacatccac tttaactcgc cctttggtgc caaattcctt ttctacgtta
    11101 aaaggaatct cgataaatgc aatattctgg ctaccttcac ggtgcagcgt ggcatcgaat
    11161 tcgtaaactt tgttttccat tattcttttt tttattccat atcctgaata gttcaactac
    11221 aaggctttag attaaaaaca gtatgtctgt gcccggtatg ctccaaaaag cgtattacat
    11281 ctgtataagc aagagtaatg atagcccctt tgtagttggg taaaaaactg agcaatgctt
    11341 cctgttgcag acggtcatcg agaaatacct cggttttatg cgccgggtca ttgagtaatc
    11401 caaaaaccga aacagcaccc ggaaccactt ttaaaacgcg ttgtaacctt tggtcggaag
    11461 caaacgaaat tttccccttt ttgaaatgtt gttcgagatt gtatatagaa acaggttcat
    11521 aaaaaggagc tatgaccaga aagtgccggt cacctttatg attgcgcaga aacagatttt
    11581 tgcagcgaat agccccggtt ttcatccaga aatttgcatc atcttcatcg acaaaatcgg
    11641 caggtgattc aaaataggaa taacggattc tcagggtttc gagtgtggaa aaaatctgct
    11701 tctgaaccgg tttatttttc tcaatcatat tattttgcaa cgatatggct gaaataatga
    11761 tcaatcaggt gttttgcggc aacaaaggag cttatttcgt ttgccagcac tttttgttga
    11821 taggctttta tcaattgctg tatggcttca ttttgataaa agtgactaag cagattgtcg
    11881 tcgatgcttt gagtcatcca gtacaatgcc tgcttgatgc ggttgttttc gaaataggaa
    11941 ttattacggg taaaactcat gtattctgta atcatttgcc aaatttcggg aataccggca
    12001 ttgttgattg ccgaacatgt aagcaccctg ggtttccatc ctgattccga aacagggtag
    12061 agctgtaatg cacttttata ctgggctttt gccatattgg ccttatccac attgttaccg
    12121 tctgccttgt tgatggttaa cgcatctgcc atttcgataa tcccccgttt gatgccctga
    12181 agttcgtcgc cggcaccggc cagcatcagt agcaagaaaa aatcaaccat actgtgtacc
    12241 acagtttcgg attgccccac acctacagtt tcgatgaaaa ttatgttgta tccagcggct
    12301 tcgcacagca ctatagtctc gcgtgttttt cgtgcaaccc cacccagtgt acctgctgaa
    12361 ggggaaggcc tgataaatgc attcgggtgc accgaaagat tttccattcg ggttttatcc
    12421 ccgagaatgc tgcctttgga acgttcgctg ctggggtcaa ccgcaagcac ggcaatttta
    12481 tttcccttct ctacaaggtt agttcccaaa gcttcgataa aagtgctttt gccaacgccg
    12541 ggaactccgg taatacctat cctaacagag tttgaagcaa ccggtaaaca tcgctctatc
    12601 agctgttgtg ccatttcctg atgatcagga agcttacttt ctactaatgt gattgcctgc
    12661 ccaaggatgc ttcggtttcc acttatgata ccgttaaaat attcatcggc tgaaagcaag
    12721 cgtttcctga ccggtttttt ctttagcaat gggttaacct gtggcggctg aacaacaccg
    12781 tcgttgattt ttaatgcgct gttttcggca ttgcattctt ccacatcgcc tttgaacttt
    12841 tttttattcg acataactac gcataactgt tggttgaaag atagttcccg atgtattgcc
    12901 caacccctga attcaggttg gtgaagacag tgtgataacc caccgaacgc agtttggaaa
    12961 tatcagcgca tgtatagtac tgatatttat cgcggatatc ggctggagtg tcgaaatatt
    13021 cgactgatag tggctttttc aacgcatcga aacatgcggc tgccagttgg ttgaaactgc
    13081 ttgcagtacc ggtgccgagg ttatatatgc ctgaatgttt gcgtaaaagc ataaaaaaga
    13141 atagcacatc tacagcatct tttacgtaaa taaaatcgcg ctgttgttct ccgtcagcca
    13201 catcactacg gtgagaacga aaaagccgca tcctgcccgt ttcatggatt tgcctgtatg
    13261 cgtgcatcac caccgatgcc attcgtactt tgtgatattc gttcggacca tacacattga
    13321 aaaaacggat acctgcccag aaatatggtt ttcgctcctg ggcaagaacc catctgtcga
    13381 agtttagttt tgattctccg taaggattta gagggtgtaa cttttctacc agtgaatggt
    13441 catcgctgta gccgagttca cccattccat agcatgcagc tgatgaagca taaataagag
    13501 gtaatccaaa ttcgacgcat agtaaaaata cttctttgct atagtttaaa ttcaaacggt
    13561 cgaaaactga agtgtcgaac tcagtggtat tggtacgggc gccaagatga aagacaaatt
    13621 cgactaaacg gtgattctgc ctgagccatt cactaaacaa atggcgttct acaagctgtg
    13681 tatacttttt tgcatggtaa ttcgcaagtt tgtcattacg gctaaaatcg tcgacaagca
    13741 caaggtcgaa gtagccttcg ttgttgagcc tcgaaaccag attgctggca ataaaacctg
    13801 cagcacctgt tataactatc atctcaggtt atgtttttac aaaaataatt aaattatctt
    13861 atcgctttaa gataatcgta gaacgaaacc ggaaaatcca acggaatgtg cattgaattg
    13921 cttaaatgga aatgttacca aaccgcgtat tgaagccata tggaaaagca tgataaaaaa
    13981 atagctatca ggataacaat acacttgccg gggcatgagt gatttcaata ataaaattgt
    14041 atttttacga tatgaatccc gatgaatgaa aaaggaaccc aaaaggctta ttgctgttag
    14101 tattctgttt gtgctggcgc tagcttcgat tataacaggt agcttgctgc aaagtagaag
    14161 tcaatcgctt cggaatattg ccaaagaact tagcagtgtt gttgtcggtg caaaccagaa
    14221 tgcagagcag atatttcatt tgattgaaga tgccggtagt tccgaaaaga gagactcggt
    14281 agttttttac agttttactg agttatatcg cgatcaaaga gctggagttt ttgtgtacga
    14341 cactactgga ttgcgttgct ggaccacaaa tcgaatacct gcaccgcttg cagcagatag
    14401 tatttatgta aacaatgctt tggttaggct gagcgatggc tggtatttta cagcgcaaaa
    14461 acttagcgat gattatcgca ttttatattt tttattgatc aagcacgact attcgtatga
    14521 aaacgaatat ctcgacaagg atttcaaccg gtttttatcg gtacccgaca atgtattatt
    14581 ttcgattgac agtatcgaaa attcagaacc agtgctatct ggcaaaaccc cgttgtttta
    14641 cgttaccatt gacgatattg ctgcaaccac gggtggtgca tttcttctca cgcagggtgc
    14701 aggttttatg cttttgattg ctttgctggc actgttatgg cagatttcgg cgatacttgt
    14761 aaggaaaaat atttcggtgc tgtttgttgc tttgggtttt gcaatggttc tgaccggatt
    14821 gcgcattctg atgctgcgct ttcaaattcc ttctgcactg tatgctttgc ctgttttttc
    14881 accggtattg tatgctgaat cgttctggta cccatcgctg ggggatttgg gaatcagtct
    14941 gttgtttttt tgtgtgtgga cactgttttt tatgaggtat tttaccatca ggccgtttgc
    15001 agagaaccgc tattcaaagg gaataaaacc attggcaggt gtttcggttg caatagtagc
    15061 tttttcagca catttattta tcagtgcccg aataagcagt cttattctga acagctctat
    15121 tgaactgaat ctggtccata tcctccgctt ctcagcgcat agtgtactcg ccatcggaat
    15181 tgtaatacta ctgttacttt gcacagtaat acttttcgac aaaataatac gggagctgag
    15241 gtcaatattc catccgatga ttttcattat cagccttgca ttggtttcaa cgctttggta
    15301 cctgtttgcc ggagccgaaa cggaggcatt tgtgctggtg tttctgtatc tgggaattat
    15361 gagcattatt ttattagtac gcatccgtta caaaatatat aactggtatt cctttcttgc
    15421 cttgctcatc gtggcttcat tgtatatttc agaacacata tcgtgggttt cggatgcaaa
    15481 agaaattgaa gcgcgtaaag ttcttgccaa caatcttatg aatgagcgcg atgtgggagc
    15541 tgagtttttt attactacta tcgatggctc aatccgtaac gatacacaaa ttgagaagtt
    15601 gattcataat gataatacag atagcttata tacctatttt tctcaaaaat acattaaagg
    15661 atatctgaat aagtatgacc ttcaggtttc ggtgtgcaag tttaacgaca gcattctgat
    15721 acaaccggat aacaagttgg aaaactgttt tacttttttt ggcagtctgc tttccgaaaa
    15781 cggcattcct attccaggga caaattttta ttaccttgaa aatcataccg gtagaatttc
    15841 gtatttcgga tactacacgt atcctgatga tggtaattct ttgcttcagc tgtttgttga
    15901 attgaatatc aaggttttta gcgaaggatt gggttatccg gagctgttgc tcgataaaaa
    15961 atttgcagtg aaaaaaaccc taaaggaata tagctatgcc aaatataacg atggagcact
    16021 aattctgaaa aaaggcgatt acgaataccc gatgaaagtg aaaaagcgtt ctggtaaaga
    16081 cgattatcga gttattgttg aagactctta tagccatctg tactattatt ccggcgacga
    16141 taaccttgtt gtactgagta ttcctgaagc aggttttaaa gggcactttg tctcattctc
    16201 ctacttgttt tttgttgttt ttttattgtt cagtgttttc aggctgtttg tttatttccg
    16261 cgatagaaag ttcatcgata cgaacagtct taaaaaccgg atacagtttt caattgtcag
    16321 cataatactt atttctattt tggtcattgg ctttatttcg gtgtatttta ttgtggatgg
    16381 atacaccgaa aagcataatc agatgctgag agataagctg caatcggtac tcgttgaaat
    16441 tgagcataaa ttcaaaagtg aagcagaact tgattccgca caaaatgact atgttacatc
    16501 gttgctgata aaattcagca atgtatttta taccgacatc aatatttttg gtaccgatgg
    16561 taagctgatt acctcatcgc gcatggagct attcgaaaaa gggctgcagg gaacctttat
    16621 gcaccccaag gcatacagac agctgcatgg cgaaaagaac ggactggtaa ttatcaatga
    16681 acgtatcggt acaattaatt atttgtcagc gtacattcca ttccgtaatg ccgacaacca
    16741 aaccatcgca tacatcaacc tgccttactt ctcgaagcag gatgaattga atgatgagat
    16801 aacaggtttt ataattgcgt tcaccaatgt atttctgata cttatcatac taagtatagc
    16861 cgttgggatt attattgcca atcagcttac aaagcctttg tcgtttttgc aggaaagcat
    16921 tcgagcaatt gatatcagca aaaagaccga aaaaatcgat tacctgcgca acgatgaact
    16981 gggtagttta ataagggaat acaacaaaaa aattgatgaa ttggccgaga gtgccgggaa
    17041 acttgctaaa tcggaacgcg agtcggcatg gcgtgaaatg gcaaaacaaa ttgcacatga
    17101 aattaaaaat cctctaacgc ctattaagct tagcattcag tatctggagc atacatacaa
    17161 atacaatgat aaaagctggg aggagacctt gaaaaaagtg gcgaaaacaa ttgttgaaca
    17221 aatcgacacc ttatcggcca ttgctaccga attctcgaat tttgcccaga tgccccaagc
    17281 acaaaaacag ccgactgacc tcatcgaaaa aattaaatcg agcctgaccc ttttccagca
    17341 atcggaacca gacattgagt tccggtttat tgatcaatcg gaaggggtgt caatgattgt
    17401 tgccgaccca gaacagctta cgcgaatatt taataaccta attaaaaata gcattcaggc
    17461 tattcccgac acaagaaagg gaaatatcgt tttaagcctg caacatgcga atcactactt
    17521 cctgattagt atcagcgata acggaaccgg tattgatgag gctatgaaga gtaagatttt
    17581 tcaacccagc ttcaccacaa aaacaagtgg tatgggttta ggcttaagta tggtgaaaaa
    17641 cattaccgac caaatgggtg gcgaaatatg gtacgaaaca gttgctgatg aaggtacaac
    17701 gttttttatc cgtcttcctg aaggctaatt attggtaata aaagcaatat gaaatgacga
    17761 gttatatgga atcagaatac aggagacatc cttcgacatc tcgatttcgc tcgatgaccg
    17821 aggcttagga ttacagaatt cagaaggggt ggcttgcaat tcagcagcat ccttctcatg
    17881 atggagtttg cgttctttct cggaaatgca gggttatctt ccctaaccaa atgccacctt
    17941 ctgtattctg cattccccaa ataataatac gtcactggta agaatagtta attcatcggg
    18001 aactgaatgc ttacaccaaa acgaagttta ggcatctgcg gataaggatc ttttacgtag
    18061 ctaaacggat tatattctac aaataatcca aatggtgcgt atccgaattt agccatcagc
    18121 gaaacgtcgg tatatttatg aattttgttg gtagcggttc gggtatgatc gagtttgtat
    18181 ttataagtaa atagcaaagg gaaatgataa acagcaccca gatcgagata ccagccaaaa
    18241 accggagctg ttttgaggtt ttcggaagaa accctgaaaa gcaatccttc acttaaagtc
    18301 catttgaaga aaccttcttt gtcgttcaat gcgggagtaa catccatatt gtcttttact
    18361 ctgtaaaaat agaatccggt acctacggta ttgattattg caaaatgttt ggccacaggc
    18421 attacaatat tgtattcgta tccgaaagca atttcacgct ttataggata attactgtct
    18481 tcaagcaccg gaaaactgaa acggaagggt agcacaaact cgatgtatgc cggaaattct
    18541 tcgggcaccg aatcattaat tatttctgca acaggcagat taatgtctaa atacgtagta
    18601 gaattctcct ccaccaatac attattataa gttagtaatt cctctccggc actggttata
    18661 acaatgcgat aaacatcgcc aggaatgttt tcgaaaaaca aacagtcatc ggaggttttt
    18721 gttgtaacca gttgtgtgtt gagccatagt tccacgctta aactactgtt cgatgcatcg
    18781 tcatcatcgt tgcccagtat gtttagctca atattacctg tttggccgat tgctacaaat
    18841 acaatataaa ttgccagcaa agtcatgata aattttttca tagaatgaat ttttgtttgt
    18901 aaatgtaact taattaaagt taatttaaaa actaattatt cgaacttttt agcatttcat
    18961 ttgttacatt tgatattatt aaccttaaac caaccattat gaaaaaatta atttacggta
    19021 ttttgttaat aggatttgtg ctttttctga attcatgttc tgtgcagaag caggtaatca
    19081 atgatcctga agaagtaatt caactacgaa tgagtaattt ggatgatgga actaatggta
    19141 gcggtgttgc ctataattct gatttaaaat tgtactacac tgtaattgcc ggcaatgccg
    19201 agttccctct ggaagttttt gaagagaatg gtgaaaatgt atatactgcc agtgccggag
    19261 tcgattgcag agggctttgg tacaataaat cgacaaagac gctagaagca aatgtatatc
    19321 aaggtggcct atataaaatt attatatcgg aggagggtat tcctacgggg cagatagctg
    19381 ccgtgtttga tgggggagaa cagccataca gtaattcctg tgcagaatat aatttcaaaa
    19441 gcggcaaatt actttactat tatagtggaa cagtttatac ggccaatcgc aaaaacggta
    19501 aaatcgagag taaaattaag ctggaactcc catgcaatat tgaggacatt aatacaacat
    19561 caattattta taccggttat aagggtatgg aaataggcat tctaaactgc ggacagtgga
    19621 aattatatct tttcaacctc aaggatggct ctttaaaggc aacagttaat atccctgatt
    19681 atatgacggt tgactgtgca tttatgtttg actttgcgaa tgattatgca tttttctata
    19741 atgtaaacga tcgtatctgg tcagggatca agatttttaa gtaatcaggt ttgtgcaaaa
    19801 atgactttgt gttttaaatt tcaggtgagt agccttttgt ttacaatata ttgaaataga
    19861 aggctttcgc caatgtcgga aaataacgtt ttactctaaa aaataaatct gattggtgaa
    19921 gatataaagg tgtgcactaa aacaaaaaat gcccgtttag ggcattttgt atgaaaagat
    19981 ttcttttatc ttcgattgta tcccgtatgt ctttggcctg tttcttcacg gggttgggcg
    20041 attttaacca caattacgct tttgtcaaac tcggtttcgt tgagcttgct tattgcctcg
    20101 ttagcttcat tttcattgct catttcgaca aaaccgtaac gctttgaaaa gccggtgttt
    20161 ttatcgataa ttacttttgc tgaagttact tcgccatagg ctgcgaataa attttttaag
    20221 tcttcacttg tagtggaagg gcttaatttg gctacaaaga tgttcataaa aaataggttt
    20281 aactgttaat aattataaca gcagtacgca acaatacttt tcatggtgat tggttgtttc
    20341 ttactgttac tacaaagaaa cataaatttt taatacaaaa agcaattatt ggtttaaatg
    20401 tttgctcaga ccgttataaa aatgaaaaca aagtacagtt tctgttcaca agtgcatgca
    20461 ataaacttta acgttttttg tattttggtt taaagcgagt atccgacctt gcttcacctt
    20521 tattgaagtc acctggcttt aaagagggct tttctgtatc aattttaaca atgattttcc
    20581 tgtcttcaag ataaatgtcg ctaaaagctt ctatcaccat ttcaaccaca tttgaatcga
    20641 gctcaaaaat gctatattta ttaaacaaat caatctttcc gatattgagt tttactccgt
    20701 tcacttcttt acgaagcagg tcgataagca gatttggata taaaccatcg ttgcggccta
    20761 acccgataaa cattttagta tatgctttgc gttgaaagtc gcggttatcg gggatgtctt
    20821 ttttaacacg tttttccgct ccctgatttg ctccggagtt ttgatatttc tcttcgttca
    20881 gattgaggtc ttttgtgttt ttataataga gaaggaatct gttgaattca accgatacaa
    20941 gccgtttaat aagttcctct ttatcgagcc attcaagttt tctataaaca gctggcaaat
    21001 aaggctctat ttcagcatgt tctatttcaa ctttttcaat cctgtcgatg agatggtaga
    21061 gctgtttttc acacacttct ttaccgtttg gtacttttgc ctgaataaat tgtattcctg
    21121 aattcttttc aattaatctt atttgagact tttctcttaa attggtcagc acaaccgata
    21181 tccctgcttt cccaacgcga ccggttctgc cactgcggtg tgtataagca tctttatcat
    21241 ccggaaggtt atagttaatt acatgggtga gattatttac atcaatacct ctggcagcaa
    21301 cgtcagtagc aacaagtatc cttacatttc tgattcggaa tttttgcatt accgtatcgc
    21361 gttgtgcctg cgataagtcg ccatgcagcg attctgcatt gtagccgtct gccatcagtt
    21421 tgtctgctac ttcctgggtt tcgcgacggg tgcggcagaa tataatgccg taaatatcag
    21481 gatagtaatc cacaatacgc ttcagtgcca aatatttatc tttcgcttgt acggtataat
    21541 tgaaatgccg tatgttttcg agtcctgtat ttcgtacgcc aatggtaatt tccactgggt
    21601 tattcatgta cttattggca attcgtgcaa tttcgggtgg catcgttgca gagaacaaca
    21661 ggctgttgat tcctttgggc aaattttcga gtattttgtc gatgctttca agaaacccca
    21721 tattcaacat ttcatcagcc tcatcgagaa tgattcgctt cacttctttt aaattgacca
    21781 cctttcgttc aataaggtca ataagtcgac cgggggtagc aaccactata tgaacgcctt
    21841 gatggagagc tttaatttgg gtttcgatac ttgagcctcc atacacggca agtatcttaa
    21901 tgccatcgat atatttggca taatctttta ggtcgtcggt aatctgaagg cataactctc
    21961 tggtgggact taaaatgagt gtttggggtt tgtaaatctc aatttttgtg ttttgaataa
    22021 tcggtaaccc gaaagctgca gttttgccgg tgccagtctg agctaatgcc agtacatctc
    22081 ttgtttcatg caacaacagg ggtataacct cttcttgcac gggaagtggc atttcaaagc
    22141 cacaatcttt aattccttta ataatatcgg gttgtatccc caattcttcg aacgttttca
    22201 tttttttttg tgtaattaca ggattgtggg taagttaagc atcctgtttc atgttaataa
    22261 ttcatccgtt tggtaattaa cttatacgga ttgtctcaaa tagttttaaa tttcgtggca
    22321 aaggtagttt tatttctgaa tgaagagtta tttatttttg catttatggt tatataacaa
    22381 aaactgcctc gcagagaggc agttcgacaa tcgtaaacat atttttagaa tgtgtaccta
    22441 accgataggg cgacacttgg gtcgaatggc gacatataat gaatgtcgtc tctgtcgtac
    22501 cacggtagaa aatggaacat gggtcttgaa tcgagactta ccagaatggg tacgtcaagg
    22561 gtgttgaagt cgtattcaat acctacctgg ccaccaacgc cgactccgag ctttacctcg
    22621 tcgagataca ttcctacaaa tgctccgggg ccaatgtacc agttcaagcc ttctacaata
    22681 ttccagtgcc agtgatatat tccggagaaa aacacacggg tatggtacaa acctccattc
    22741 cagcccaaat ccagctccat tctgtgagat tctcccaacc catgctggta cgatacttca
    22801 gcaccataat aagtaccacc gccaaagcga acgccaatag catgcggatt aacctgcgca
    22861 tttgctgtag caaatccagc cacaaccaaa accaataaaa aaactaattt cttcatcttt
    22921 attctttttt cgattattat tatgcaaaat tataaaattg attcgttatt ccgaaatatc
    22981 ccgaataatt atcagttaat gtattaacag agtaagtgct ataattgtaa ttatattgcg
    23041 gagtctcttt attcaccgta taaaacgatt tattataatg ttaaaataat tctatttttg
    23101 tagtgattaa ttccttattg tatgagcaaa tattttaaaa cagccatttt accgcacttg
    23161 atagcaggta ttatatttct tattatttcg atagcatatt tttcgcccgc atttcttgag
    23221 ggcgaccgtc tgaacatgca cgactggaag gtgtggcagg cttcgagtaa ggagaaagct
    23281 gaatttcaga atcaaacagg cgaaattacc aactggacca acagcatgtt cagcggtatg
    23341 ccaacttacc tgatttcaac tcccaaggag caaaacgtat ttacttatgt tcagaaaatt
    23401 atgggttttg gttttggaga gccggtaacc tttttgtttt attatctgct gggcttttat
    23461 attttgcttc aggtatttaa agtagatgtc cggctagcca ttgccggagc aattggattt
    23521 ggcttttcat catatttttt tgttattatt acagcagggc accttaccaa agctgctgca
    23581 atagggttta tagccccaat tattgcaggc gtgtatctgg cttacgaccg taatatgtgg
    23641 aaaggaatgc tcctgatgac atttgcgttg gcattgcaga tacttactaa ccatctgcag
    23701 atagtttatt ataccatgat gataattctg gggtttggaa tagcctggtt tattggtgct
    23761 atcatggaga aaaccctgct aacatttctg aagactaccg gagttctcat cgccggtgca
    23821 gcgctggcag ttggggtaaa cgcaactaat ctgattacaa cacaggagta tgcgccatat
    23881 tccatgaggg gacaaagtga acttaccaat gcacaacatt tgcgaaccac gggtttaaac
    23941 agagattata tcactggatg gagctatggc aaaacagaaa catttaccct gttgattcct
    24001 aatgcaaaag gaggagcatc ctatgcacct ttaagcgatg aatcagaaac ctataatgta
    24061 ctcgcaaaat attacggtga agatgttgcg ggtgatatta tcaaacaaat gccggtgtat
    24121 ttcggaccac aaccctcaac ttcagggccg gtgtatatcg gggcaatcgt tttctttttg
    24181 tttattctct ccttgtttgt ggttaaaggt aaaattaaat ggtggctgct gtcagtaacc
    24241 atcctgagta tccttctttc atggggcagg cactttatgt ggttcaccaa ccttatgctc
    24301 gattattttc cagcctacag taagttcaga acggtatcga tgactctggt tattgccgag
    24361 tttacaatgc cattactagc atttctaggt ttgcgcgaaa tattttcggg taaagtggaa
    24421 cctaaaaaac taatgaatta tttctatctg tcgataggag taactgcaat gatattactg
    24481 gtgttcattg cataccccaa agctaccggt aatacaagtg agcacgagga gaaatttgta
    24541 tcacagcagc ttctggcagg cattccagat gaccctaaat acgatcaggt taaacaggac
    24601 attatgcagg agtttaccac ggctatccaa accgacaggc tcagcctgat tcgtaacgat
    24661 gctgcccgtt cattggcatt tgctgtgtta gccgccttgg ttgtttttgt ggtgatgaag
    24721 agaaaaatga aagcagtata tgcggcaata atactcgcag ccctgattct tgtagacatg
    24781 tggacaatta ataagcgcta tctgaatatg gataattttg tttcagaacg cactttcgaa
    24841 gtcccctata cagcatcacg ggctgacgaa atcattcttt ccgacaagaa ttccgatttc
    24901 agggtttgca acctaagtat ggggttgagc ggtgtatttg ccgatgcatc aacgtcgtat
    24961 tttcataaat cgataggcgg ctaccatgga gccaaaatgc ttagatatca ggaactgatg
    25021 gattcagtga tggcgggtga actttacatg gctgcatata tgattaatgt tgccggacag
    25081 aataaaatgt cgtttgccga aatgcagatg cttttcgatt cttcgggcaa tttccctgta
    25141 ttgaatatgc tgaataccaa atatattatt tacagccccg aaggcgcacc aatccggatt
    25201 aatagcgctc ttggcaatgc atggtttgtt gactcgtatt ctttggcaga agatgccaat
    25261 caggaaatac tcatgatgcg caaactgaac accgcttccg aagccgtaat tgataagcgt
    25321 tttgaaagtg aactgaaggg attgaaaatt acccctgaca gcactgctgc tatacgtttg
    25381 gtagaataca aacccaatta tctgaagtat gaatcgcaat cggcggcaga cgggttggtt
    25441 gttttttcag aaatctacta ctctaaaggt tggcaagcaa ccatcgacgg aaaacctaag
    25501 cagcatttca gggctaatta cgtactccgc agcattgtcg tccccaaagg aaaacataca
    25561 attgagttca ggttcgaccc acagtcatac aaaacgggga atcttatatc ctatatttgc
    25621 tcaggaatta tctttttaat gataattgca tcggctgttt tgttttatat aaaaaggcga
    25681 aaaggaacaa aaacaacctg acagggaatg ctgttacatt ttattttgac aacctcacag
    25741 atgtcattac caaagcccat tcatcagaaa gctggaagca gacctacttt gatttaagtg
    25801 ctttataagt tctgtaatcg gcataattcc aggataacca atgccgtgat tcatatttct
    25861 tgcaaaagtt ggctttttta ctttggatag cactgtaata cgtgacataa tccatgtatt
    25921 ttactctatc ctctacatca aatggcattt tatgtttctg cgtctcatga atgacctgta
    25981 gttcctctgg cgttttgtca agctccggca gggcattagt actcattttt tcaaggaaat
    26041 tgaaatgtac gaatgacctc tcataatggg agaagttgta ttttgcgatc atcagatccc
    26101 aattaataaa aaccgaaatc aataaaaccg aaaacaaggc aatgctgttc acacgaacga
    26161 gccaataccc tgttttacga tggtatattt ttacaattac actgataatt ccgataatta
    26221 caagcaacag gaaaaacaaa actgcaattc ttttataagc cagtgcaaaa tagtttatat
    26281 accataaatt tctaattact accgaaatgg tcattataaa attctgaacc atccaaagtg
    26341 ttgccaacac ttttaaccac ttattttttg aataaaaatt caggttacgt ctgaagtaat
    26401 atatcacaat aaacatggat attaaaatag atacgaccag gatccatgtt cccgaatgca
    26461 caaattgttt caggtaatcg ccattccact cgaagccaaa ccatacccaa atgatatcaa
    26521 ttacgttcag gtaaagtatc agggcattca atgctacgag aaggaaaatt gcagatttat
    26581 attcgttaat cagcgagctg aaccgaaaac ttcgacctct tcggcgaggc agtcgtgtca
    26641 tggtgtcttt agctgcgatg tcgttgttgt gcattaaaga gtgactgaat tttacaaaga
    26701 atattgtgca tagaaagaag ccgaaaacgg ccatccagaa ccatccccag gtaataaacg
    26761 aaagccagta atttattgcg tcaatcaggc gaatgatctg attgttgaaa atggggttcg
    26821 caatccggta tagaaaaatg aacaatatga taatagccag cgggattaag ccccatttca
    26881 gtacgcgccc gaattttccc cacgaaatcc tgtttttggg cttgcgggcc agaaaaacaa
    26941 agaacccaat ctgcgatgta ataatgtgag caatcgattc aacccaaagg tgcaacaacg
    27001 atcgcattgt aggaaaagac ataaaagcgg caaacataaa tgcaaatatc aaatgcactg
    27061 tgaacgacca tgccgtatga tagaaaaaaa ccgaaaacaa acttagcacg gtgcctacga
    27121 acatgaacca aaaaatgccc gttttcttaa aaggcgaata gttccaaagc attaatggaa
    27181 gtgcaagacc ttcgaaaatt agcaggttta ccccaattgc ctgacggtaa aaaagtaaag
    27241 aaaataatac cgagagacag aacattaccc aaataatact gtttttcata gcaatagaat
    27301 tagtaattgg gtataaattt aactcaatat tttgtttttg acgggttaca tacaaaaaac
    27361 tatttatgac ctgaattatt gtctttatgt tgttaaagtt ttgctaatat ttaacagcga
    27421 ttttatccaa aatattaaaa ttgaacaaat tgggttatta ttggtttaat gaataaaaca
    27481 ttaaaacgat aaacatgaaa cgaattaatt tgataagtat ttttatttta gttgccgtaa
    27541 cagttgttgc ccaacatgtt cagcaaccgc tgccttatgc ctacaatgct ttggagcctt
    27601 ggattgatgc gcaaactatg gagattcatt actcaaaaca ccataaagcc tacatcgata
    27661 atctgaacaa agctgtgcaa ggcacttcgt atagtggtta cgacttgacc aagttacaat
    27721 ttaacattac aggcgaaacc cctgctgtaa ttcgcaataa tggcgggggg cactggaatc
    27781 atgaattttt ctggaatata ttgacaactc caaatgagga taagattcct gaagatttgc
    27841 tgggtgcttt aatttcatca ttcggatcgt ttgacaactt taaagccgaa ttcaaaaaga
    27901 atgcgatggg tcggtttggt tcaggctggg cctggcttat tgttaaaaat ggcaagcttc
    27961 tgattaccac aaccccgaat caggataatc cactgatgcc ggtagctgaa attcagggcg
    28021 ttcccatact tgggatcgat gtttgggagc atgcctacta tctgaaatac cagaacaaac
    28081 gtggcgatta tgtcgatgct ttttttaacg tggtaaattg gaataaggta gccgaacttt
    28141 acaagcaaaa ttataaaaaa tgattcactc cggatttatt gtttaatggg tatgcatacc
    28201 ttgggtccat ccttttgaga tcatgaattt ttcgaccctg tgaatcagtt cataattttt
    28261 aagtcctccc caacaaggag caatcagcat ttcctgcggc gattcgcttg aaaatctgtc
    28321 aggcattatt ccaggtctta catagcataa aaattcagat acaagggcta catattcatc
    28381 aataccgaat agggtaaact gttccggttt ttcgcgaaac atgctttcag caacagtacc
    28441 tttaattatt tgaagctgat gtattttaag gttgtgaacc ggtaaatccg aaatattctt
    28501 tgcatgcaaa atcatagtgt cgcgatcttc atcgggtaaa cccaaaatta gatgtatacc
    28561 ggtgtaaata ccattttcat tgctgcgtgc tatagcttca accgattgtt cataagtgtg
    28621 aaaccgattt attcttctca gcgtttgatt gtttgtcgat tctacaccaa attcaattac
    28681 cgtatacttg tcttttgcaa tcagcgaaat ttctttgagg agttcattgc ttacacaatc
    28741 ggggcgggtt gcaattacca atccgataac attatcgaca gatagcgctt cctgatataa
    28801 gcttatgcaa tgctcaattt ctgcatatgt attggtatat gactggaaat atgccagata
    28861 attctgagtg cgatattttt tgctaaaaaa agcaattccc tcgtttagtt gatgggtaat
    28921 tgttttttta gggttacagt aaaatggact gaaagtttca ttattgcaat atatacaccc
    28981 ggtttccgat aatgtcccgt ttcggttggg gcaggtaaaa cccgcatcca ccgatatctt
    29041 ctgaaccctg ctcccaaagc gttttctaag gtaagcggca taatcgttcc aaggcttagg
    29101 ctgtctttcg acctgcattt ttagtttttg ttttcgttgg gtttgtaatc gtattcagga
    29161 gaaatgcgat gtgttgcaga tgtattcgaa ggagctcctt tttcgttatt actttcatta
    29221 acatcatctt catcatcttc ttcttcccat gaatatcgtg gccgttgggg acccttacta
    29281 cgataaataa gggcaaggac aaaacctgta aatagtcctg ataaatggcc ctcccaagaa
    29341 atgtttggtt caattggaaa gatgccccaa atcatactgc cgtataaaaa aaccaccagc
    29401 agcgatatgg ccataaggcg gaaatgatta cgaagcacac cacttagaaa gatgaagccg
    29461 gcaaacgcat aaaccagccc actggcacca atgtgatacg actcgcgacc aattacccag
    29521 gttatgattc cagttgatag ccacgaaaac aacaggactt taacagcaat atcgcggtag
    29581 aaataaaata ctccggtgcc aagaacaaga aatgccacac tgtttgcagc caaatgggcg
    29641 taatcaccgt gaagcagtgg catcagtata ataccgaata acgaatgcag attgcggggc
    29701 agaacaccat agttgctcaa actgattcct gaagatattt cgaatagttt aatgagccaa
    29761 caaattaaaa gtattatcgc aggaatagta gctgctagta tcagacgttt tcgctcgaat
    29821 cgaatatcga tttcctcatt catgaatgca aatataatca tttagcctga cacagattct
    29881 gctgttcttt ttttcgtatt tttgccaggc cgaaaaaatg atatgaagct tcttgtatta
    29941 aattatgaat accctccgct gggtggagga gcaggcgtta ttacgcagaa tattgcgcag
    30001 ggtcttgtaa aactgggtaa tagtgtttgt gtggtaacaa cagggttcaa agatttgcct
    30061 cctgactctt tggagaatga tgtgcgcgta atccggctgc cttcaaaaag gcgtttcgtt
    30121 tttcagtcga atccggttga aatgtggtcg tggatgaaac atgccaacac ttttctccga
    30181 aaacacctga aaactgaaaa atacgatttg tgttttgcca atttctcaat ccccggtgga
    30241 gcagtggctt taaacatgaa aagggagttc ggacttccat atacggtaat ttcgcatgga
    30301 cacgatattc cctggttttt cccaaggcaa atggtgtggt atcatttgat attgtatcat
    30361 aaaatccgcg aaattgtact gaacagcgaa gccaactttg tgcagtcgaa cgaaatgaaa
    30421 cggaatatcg atttgtttac cggaaatctg tttgcatcga aaaatcattt gatatataat
    30481 gggtggaaca gctcggtgtt tacggcagat tattcaaagc gtaaaagtga atttatccta
    30541 ctttttgtag gtaggctcgt tccacagaaa gacccgttta cttttctgaa agctgtgaaa
    30601 attgctgcaa caacaattga tttgcaagtg catatactgg gcgatggtcc tttgcgtgca
    30661 aaaatggaaa aatgggttac gaaaaatcac ctgtcggaga aggttaaatt ctttggatgg
    30721 gtcgacaaac ccgaaatgct ataccaatat caatcggctg cggttacagt gcttccgtca
    30781 ttatcggaag gaatgtcgat cgcaacgctt gaagctcttg ccagtggtca gtacgtgatt
    30841 gcaacacgtg ttagcaacaa cgaaagtttg attgttgaga atattaatgg taaccttctc
    30901 aacatgaaag accatactat gttggctgat ttgattgtga aattctatca tgaaaaattt
    30961 ttgaaatatt accggatccc acccgaagaa ctcgaaaagt atcgcagcct gtatgaatgg
    31021 gatggtattg ttgaacaata caacaatatt tttgcaagaa ttattggaag ctagtatttg
    31081 ttttgtttgt tgtgatgatt caaaaatatg tttttgggga aaatattttc tgattgtaaa
    31141 cactttcttg agtgaattgt tacttgttaa agagataaat atagggtaac tgtggaaaat
    31201 acaatttata atttcactgc aatttcctga cttcgacact ttttaatagg caattttgta
    31261 agtgtcagat actgaat
//
LOCUS       DAOOQP010000022        31104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1685_length_31104_cov_5.540500, whole genome shotgun sequence.
ACCESSION   DAOOQP010000022 DAOOQP010000000
VERSION     DAOOQP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_31104_cov_5.540500"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1607
                     /locus_tag="PLK75_04030"
     CDS             135..1607
                     /locus_tag="PLK75_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38445.1"
                     /translation="MEKFAHYNVFIIITLITFFALSMGCEKNNFTENPDDKLIFSEDT
                     IQFDTIFKSIGSTTHFFTVYNSNESKSINIDRIYLAKGNNSSFRLNVNGIQSSSVTNM
                     VIAPGDSAFIFVEVTIDPGRDEMIEQDSVVFLSNGNIQDVDLIAFGQDILLINGQIFS
                     SDTVWTNNKPVLIFNSALFDSNTTLTIEAGTKVHFHKNSSLFIKGSLLVHGAKENHVV
                     FSGDRLEDEYSEISGQWGAYQTDDVGNVTALYGGIHLLQGSANNIIEYAEIKNGIIGI
                     RVDSVTTPGTPSLILRNTSIENMNIAGLYGLGSHIEAYNCVFANCGKYTVVCAIGGNY
                     SFYHCTLANFWSGSRQTPQLLLNNYYNYSSHGSTITEYRDLTNAYFGNCIIYGNREQE
                     ISLDLETGAEANFIFENCLLKTSSTFNTSNTSYFIDNILNVNPKFIETVSPYNYELDT
                     LSPAKDAGKLSVSLLIPFDYLGNNRLSDAGPDLGAFERIE"
     gene            1604..2692
                     /locus_tag="PLK75_04035"
     CDS             1604..2692
                     /locus_tag="PLK75_04035"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOY38446.1"
                     /translation="MSKLLFVISIVISIAVISCRNGTKEIAVSDAYNEFSKTEADRFR
                     FMFYNVENLFDIYNDSLKNDDEFLPDQAKYWTASKYYTKLNHIYQVIVAVGEWELPDI
                     IGLSEIENRIVLEQLIEKTPLYKTKYKIIHYESPDPRGIDVALLYKPSSFKPLYSEPI
                     RIVFPGSTSGGTRDILYVKGIINNNDTLHIFINHWPSRWGGQMETEDKRMHCATVVHH
                     KTDSILKANPNSNIIISGDLNDYPTDKSLQECLKAQSSLEKIENKKLYNLSYYLQETK
                     HLGTHKHDGHWGVLDQIIVSGALLNGTGGLITSTENIFILDKPFILEEDEKNTGLQPF
                     RTYAGYKYLGGFSDHLPTYIDLKKNKPQ"
     gene            complement(2725..4200)
                     /locus_tag="PLK75_04040"
     CDS             complement(2725..4200)
                     /locus_tag="PLK75_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOY38447.1"
                     /translation="MKKIIFFVAALSVSFQMLIAQNEVDALRYSYLVTGGTARYTSLG
                     GAFGALGGDITCLSTNPAGMAVFQQSDFSITPSFEYINSEASFMSSTTTDFDYNFNIN
                     SFGYVGTSKFNKESSSLKNINFGIGYTRLNNFHQNTLINGDNTNNSMTDWFANRANGT
                     HFSQLQNVDNFYSSLAWETYLINQVSLTDTMNYISVYNGVYGQNTSHAISRDGYLGEF
                     DISLSANFNHLLYLGATFGIQPLRFEQTIFHSEVDSDDAIADFNSFNYTEHLYTHGTG
                     LNFKFGLLARPVDWVRIGAAIHSPTFYNLSDEYSTEITSNFVNPDYSRTWYSSAGYYD
                     YQLTSPFKAIGSLAFVIQKYGLISFEYEFVDYSLARLRAYDYTFIDENQAIQTEYKST
                     GNIKGGLEYRLGQLSLRAGAAYFGSPYVKGHINEGSYQMMYSGGLGFRGKSLYFDVAY
                     AYITGNEGYLLYESSDPVLTSPVSDISKNSNRILATIGVKF"
     gene            complement(4227..6014)
                     /locus_tag="PLK75_04045"
     CDS             complement(4227..6014)
                     /locus_tag="PLK75_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38448.1"
                     /translation="MKTHVLLFVFVLLLVATSCTTSFYTSVGGEYDDVYMTKSDNNNN
                     PQNAVTDNSVDEDKAVYETVYQEEFDSGNTYSEDTTINEYYNQGENYSESSDYYNYDY
                     PDGNSYSDTYYDDGSTIINNYYYYDDYYDSYYYRLNPYYVYHYDPYYYDWNWGFSWYY
                     GWGYSYYPYYWSWWYPYSWSGYYWGWGSPYWNGYSHGYWDGYYDGYHDNYVYNDYNDW
                     SDYGSNVYYGHRGSVGGNGSSSSNRLPENTDNLNTPMNINSNTNNATNLGREVDPNAN
                     EKPSGNFDRPVKANDNEAVNNKPVDKPTDKPINNKPAETPQTKPVDKPDNNQVTRPTN
                     PSEKPGYNNNQPTKPVDKPNNGINQPSKPADRPDNNQVTRPTTPAEKPGYNNSQPTKP
                     ADRPNNVGNQPTKPAERPNNNSYNQPSRPSEKPNYNNNSGNSNQNQVKPNNPSGSQSG
                     SSPRNNSYTSPTKPSTPPANSGNTNKVTTPDSRSTNPPKSGSGTYNRTPSSGSNSGTY
                     NRTPSSGSNSGSYNRTPSSSGSNSGSYNRGSSSGSSSGSYNRSSSSSGSSSGSSSGRS
                     SSSGSSHSSGGSSGGSHSSGGSHSSGGRR"
     gene            6095..7567
                     /gene="proS"
                     /locus_tag="PLK75_04050"
     CDS             6095..7567
                     /gene="proS"
                     /locus_tag="PLK75_04050"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOY38449.1"
                     /translation="MAKELTSRAENYSQWYNDIVVKADLAENSAVRGCMVIKPYGYSI
                     WEKMQSALDTMFKETGHQNAYFPLFIPKSFFAKEAKHVEGFAKECAIVTHYRLKNDET
                     GNLVIDPEAKLEEELIIRPTSETIIWNTYRNWIQSYRDLPLLVNQWANVVRWEMRTRL
                     FLRTAEFLWQEGHTAHATQEEAIDETKKMIGVYADFAKNWMALPVHIGTKSDNERFAG
                     AIETFAIEALMQDGKALQAGTSHFLGQNFARAFDVKFLNKDNKEEFVWATSWGVSTRL
                     LGALIMAHSDDNGLILPPKLAPLQVVIVPIYKNNEQLEQIGVTALNIAEKIRKTGISV
                     KYDDRDNLRPGFKFSEYELKGIPVRIAIGPKDMENNSCEICRRDTLEKYNISQDDVIS
                     HIPELLETIQEEIYNKAIARRDANTYYAETYEEFKEILENKPGFIWAHWDGTTETELK
                     IQEETKATLRCIPVNGDKEEGRCIVTGKPSKQRVLFAKSY"
     gene            7579..8301
                     /locus_tag="PLK75_04055"
     CDS             7579..8301
                     /locus_tag="PLK75_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38450.1"
                     /translation="MNNSQNIKAQIVATLKEKSSTKQIVYDQTKAAFKIIKSILQNIV
                     DEYNEALKGSDERIMLEYRDRGMFEAEIKVAGDLLIFNMHSNIFEFDYNHPIWRNPEA
                     KNEPIKTYSGIINIYNFLADSFKYQRLEDLGYLVGRMFVNMEGSFFVEGRRELGFYYP
                     AFGKNMVSYENLRMMIESTIIYALKFDLLVPPYEDVKISTVEVMKEKINKSKVKTGKR
                     MGFAYNVDLGKATTVDTYTDSE"
     gene            8525..10360
                     /locus_tag="PLK75_04060"
     CDS             8525..10360
                     /locus_tag="PLK75_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOY38451.1"
                     /translation="MAKNHSVVQAESVVVKFAGDSGDGMQLAGTMYADISALIGNDLA
                     TFPYYPAEIRPPQDTVAGVSGFQVNKGANIKTSGDECDILVCMNPASLAANFKFAKKG
                     GTIIIDSDSITPKALEKAGYKSNPLEDGSLSSFTIIKAPMTSISVDACQHLDIDNKSA
                     ARTKNMFALGMVVYICDYDLEKSFYVINKKFKGNPTLALANKAAAKAGYIFAEASEIN
                     FSRIHTPVTKIEKGRYRTITGNIATAWGLLAASEKSGRQLFLGSYPITPATEILMELA
                     KHKSLGAKVFQAEDEIGGICSAIGASYAGALACTTTSGPGLSLKTEALGLAVIAELPL
                     VVVDVQRGGPSTGLPTKSEQSDLYMALFGRNGEAPLFILAASSPSDCFIKAFEAAKFS
                     MEHMTPAILLTDGQMGQGSEIFKIPKMAELPAIKPPIVPANDKDYKPYLRNPETLARG
                     WAIPGTEGLRHRIGGLEKTNVTGNVSTDPMNHELMVKLRADKVTKIADYIPEQTIDYA
                     KSGDLLVVSWGGTKGSVSTAVGELFAQGMKVGHAHFSYISPLPKNTGKVLGKFKKIIV
                     CELNAGQFVNYLRMNFPKHDYLQYNKIQGLPFTTLELKEQFTKLL"
     gene            10370..11410
                     /locus_tag="PLK75_04065"
     CDS             10370..11410
                     /locus_tag="PLK75_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOY38452.1"
                     /translation="MSFENNFTIDRSEVPLTKEDFVSDQMVKWCPGCGDHAILASLEK
                     VFPEIGYKKENFCIVSGIGCSSRFPYYVNTYGFHGIHGRANVIASGVKIANPHLSVWI
                     ATGDGDSMAIGGNHFIHIIRRNIDVNIMLFNNQIYGLTKGQYSPTSPMGSKTKTSPQG
                     TIEQPFNPGELVMGAQGTFFARTIDTNPKHMTEVMFEAARHDGTSIVEILQNCVIFND
                     STHKLITGRDVRDDNQLLLQHGQPMIFGKDRNKGIILGKNGLQVVTIGEKGIKEKDIL
                     VHDMHQKDPGIHLMLAKLKPPTYPIALGVIRAASFPTYDDLVEEQISYAKDTAQIKNV
                     DDLLNSGDTWEI"
     gene            complement(11429..12394)
                     /locus_tag="PLK75_04070"
     CDS             complement(11429..12394)
                     /locus_tag="PLK75_04070"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOY38453.1"
                     /translation="MKFEIQILGSSSALPTSKRFPSAQVVSYNNTPMLVDCGEGTQIQ
                     LRKYGISFLQIEHIFISHLHGDHYFGIFGLLTSMNMLGRKKALHLYAPPELHKILNSI
                     WRLTATALLFEVKFHPLNSPDFVLLVETRYLMVYSFPLEHSKANWGFKFCEKQFPPKI
                     KKEAIEMYKPGIAEIHEIKQGNDLVLFDGTIIPNEELVFDSPQPRTYTYCSDTLYIKN
                     LHDRVGEVDVLYHEATFASDAEALAKATFHATASQAAMVAKCTNAKALIIGHYSTRYP
                     SVEFLRHEALQVFQPVFLAEEGTCFIIDQQTRLLSISNDEDKKKP"
     gene            complement(12443..14281)
                     /gene="rpsA"
                     /locus_tag="PLK75_04075"
     CDS             complement(12443..14281)
                     /gene="rpsA"
                     /locus_tag="PLK75_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOY38454.1"
                     /translation="MPKTKKSTKGFVFDTKEIKPLDDFNWEVLGKRDESYTLKQRSEL
                     EELYNHTLSTVHEGEVFDGTVVAMNKREVVVNIGYKSEGVINANEFRYTPDLKIGDKV
                     EVYVENMEDGNGQLLLSHNKAKALRSWERVNKAHDNDEIIKGYIKCRTKGGMIVDVFG
                     IEAFLPGSQIDVKPIRDYDVYVDKVMEFKVVKINNEFKNVVVSHKALIEAELEEQKKL
                     IISKLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEEIVTLDEKIN
                     VVILDFDDDKKRIALGLKQLTQHPWDSLDANIKVGDKVKGKVVVIADYGAFVEISTGV
                     EGLIHVSEMSWSQHLRSAQEFMKVGDEVEAVVLTLDRDERKMSLGIKQLFPDPWEKIE
                     TKYPIGSKHASKVRNFTNFGVFVELEEGVDGLVHISDLSWTKKIKHPAEFTSIGADLE
                     VVVLEIDKENRRLSLGHKQVEENPWDVFETIFSLDSIHEGTIVEMNDKGSIIALPYGV
                     EGFATPKHLVKEDGTPAKVDERINFKVIEFNKDAKKIFVSHSRIHEDEKREKAEEERK
                     AKQKESGTAPRKTKPASDKIEKNTLGDMVDLDEIKKELAKKQKSKE"
     gene            complement(14619..15875)
                     /locus_tag="PLK75_04080"
     CDS             complement(14619..15875)
                     /locus_tag="PLK75_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOY38455.1"
                     /translation="MARDVTSVSKYYAISIIGMLFFIFGFVTWLNATLIPYLKIACEL
                     TNLESLFVAFAFYVSYFVLALPSSWVLGKTGFKNGMSLGLIVMSIGSIIFIPAAMMRF
                     YPLFLTGLFVQGAGLSILQTASNPYVTIIGPIESAAKRISIMGIANKIAGIISPLILG
                     TIVLKDADTITDKITTMSLAQRTAELDLLSQRVIVPYIVIAVVLLILALLVKASKLPE
                     IDKSGDSDKSGISRSSIFKYPYLWLGVLSIFLYVGAEVISVDTIINYGKSIGIKLETS
                     RFFASFTLFAMVAGYLIGIAAIPRLISQSKALFYATALALVLTVLAVVTSGITSVIFI
                     ALLGLANAVMWPAIWPLSIEGLGKYTKTGSALLIMGIVGGATIPLLYGALSDLHPIGT
                     HYAYLIIIPCYAFIMFFALKGHRYGK"
     gene            complement(16017..18014)
                     /gene="ligA"
                     /locus_tag="PLK75_04085"
     CDS             complement(16017..18014)
                     /gene="ligA"
                     /locus_tag="PLK75_04085"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOY38456.1"
                     /translation="MDKQKAFERITYLRKELEHHNYLYYVLSQPQISDYDFDVFMKEL
                     VQLEQAFPEFDDPNSPAKRVGSDLSNRFTPVKHISPMLSLGNTYSREEVEAFHHRVVK
                     ALGLQPEYVCELKYDGISISLHYENGRLQRAITRGDGLQGDDVTENIRTIRSIPLTLI
                     GSDYPEVFEIRGEVYMPRKVFNQLNEQRSSSGEALFANPRNAAAGTVKLLQSSEVARR
                     KLDCYLYYLISDQLPYNEHFARLKKAREWGFRIPEHIVKCSGIGDVFDYIDYWDQERK
                     NLPFDIDGIVIKVNSLEQQELLGFTAKTPRWAIAYKFKAEEAVTELISVDYQVGRTGA
                     VTPVANLKPILLAGTIVKRATLHNADQMSTLGLRIGDRVRVEKGGEIIPKITGIALQN
                     ESALPIEFITHCPECGYLLQRNEGEAKHFCPNDDQCPPQIKGKIIHFISRKALNIDSL
                     GEETVELFYNKGLISDIADLYTISRNDILPLERFAEKSADNIIESIAKSLEVPFHRVL
                     FGLSIRFVGETVAKKLAEAFRNIDALMSANTEELMAVDEIGEKIAESVIEYFSKPEHR
                     QLIERLRSYGLQFEQAGEHVLTHKLDGKSIVISGVFNKYSREELKKLIEMHGGKNTGS
                     ISSKTDFVLAGENMGPAKLEKAVELGVAIISENEFLAMIEA"
     gene            complement(18116..18826)
                     /locus_tag="PLK75_04090"
     CDS             complement(18116..18826)
                     /locus_tag="PLK75_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38457.1"
                     /translation="MRNIKLCAILPLILIFSNAIGQIYGRIGYNAGGIITPQFNDVVD
                     IYNENRPYLDNQMNHVVLLHGAVFGIGYHFQGSNVSNEFTWSNHHAVISSGGIDPNTN
                     EYTTRDVKYRHNCLTYGVHFRIKKFPILWWGLSVDIDNYRQFTRINDDKYSWLDKSVI
                     VEDKIYVIFDLPISDAISVQFQPYFQLPWWIAVEYDQFVDLALNVPYWDGYSLTSFPK
                     NYGLFVTFRFGGNNYGDN"
     gene            complement(18988..21186)
                     /locus_tag="PLK75_04095"
     CDS             complement(18988..21186)
                     /locus_tag="PLK75_04095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOY38458.1"
                     /translation="MNNKRNGMQEKLGLNDAAKFRKVMPERYFSVTDPEDFRQHEVFL
                     NKFEKAGNQACLYHYKGDIEEFVFHEYNTVKLREQIAIATKTLHANELLYRIFPNKQT
                     GFMLTSIYKDIMLSDRSVEFNPPVFSNEESLFFDSGRLKTHIICNYSQQNRIFEIKCI
                     NVAHKTMLSNLWETIEMHEINVDFTEVWRIKNVSAKVNSFYCITLHFASDYCLEEIES
                     VRMDFLRYIQHYTVPMSIFDLVGPSMVGPSSSHTAGANRIGQIARSIIAAKLTQGETI
                     KDISVKLLGSFRDTGVGHRTPAAIAGGLWGLATDHPDMLSHGDPEFLCENGIVIAGQK
                     LHFLGFLKGEIDEEVKYQADNNMNIAEIIFNTDIKPYTITGFSIGGGNVEIRYFNTPL
                     AKPINGKTDFYLNGDTIVDVQNYNGTLPVIQSIFGKSQPESNYIMPFNTFEELLTHSD
                     ETSMSIDEIALQVERNLQQCSEEYILQQMKYYFNIMLQSAGDGVLSKELSMLKLSGDD
                     AYKIYSLIDKSPLFCNIYGRAVAYATAVNELNAKSGVIVACPTAGSCGILPGVLKSYC
                     EFKQAEEDAMLRSLIVAGFLGMLLFNDVTTAGADYGCQAEVGAAAAMAASALCYLENG
                     NSYQIVNAFTLAIKNSLGLICDPIAGLVEVPCVKRNGIYSSVAISAALMALSGVKSYV
                     SPDEVILTMREVGERINSDYKETARGGLARTRDGKHVEKLFESEVKKFFN"
     gene            21298..21669
                     /locus_tag="PLK75_04100"
     CDS             21298..21669
                     /locus_tag="PLK75_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOY38459.1"
                     /translation="MSTDFSELKFKYKIWLETENGEGVLGEGKYRLLKKIEETGSLKL
                     AIEQLGLSYRKTWDNLRRIEEILGFDLIDRQRGGAAGGSSALTPQGKRFIEVFDKFLK
                     QHDPVIQGFLKDLKNDLQKTD"
     gene            21746..23008
                     /locus_tag="PLK75_04105"
     CDS             21746..23008
                     /locus_tag="PLK75_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38460.1"
                     /translation="MKKISFVLLLSSFCLFAAAQDTIRVMTYNLLYYNLYNSYCTNSN
                     NNVDTKDAYLKTIIAYTLPDIIAFNEVKGDNATNYRLKGSVLNSDGRNYYISTAYPYG
                     SGSLTSFLFFNKNKFYEDYELYNINTDAARDFNIYKLRVKNTNPAVYVYVIPAHLKAG
                     SYDDDIAERAQMIDSVMSKIDEMGGNDNFIFLGDLNIYSSTETGFQKLINSSNFEYRF
                     YDPINQLGAWNNNYSFRNYHTQSTSSQGDDCKASGGMDDRFDIMLVDDDIISGASGVK
                     YIEDSYATLGQDGLHFNESINNGNNNSVPSNVLNALVGMSDHLPVYADFVVGASNDIP
                     MPSANKPLVTYNNPVKEFLYVQLMATSAQQTSIRIFDITGNLIFTERLFLCNSAAIQV
                     NLSAFPSGLYLLHVESPEAVQTFKIVKH"
     gene            23027..24247
                     /locus_tag="PLK75_04110"
     CDS             23027..24247
                     /locus_tag="PLK75_04110"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY38461.1"
                     /translation="MEFNIATLSNGIRVIHLVTNSPVSHCGFIINAGTRDENNDESGI
                     AHLTEHMLFKGTHKRKAFHILSRLDDVGGELDAYTTKEETALYASFLSEYYPRAIELL
                     NDIVFNSVFPAKELQKEKFVICDEINSYKDNPSELIFDEFESLLFENHELGKSILGTE
                     NTVKSIQVSQIEKYIKRCFSTDNIVFTSVGNISFKKLLSLCQNTLQTNTKSTRNSKRE
                     KFIQSKRFTITHSFNTYQAHCLMGNTAYSYCNDKRLALIVLSNILAGPAMNSRLNLLL
                     REKHGITYNIESVYTPYTETGSFMLYYGTDEKNISKCRHLIEKEISTLCNNRLGTLQL
                     SKAKKQIMGQLALGFEIHSNLMLSIGKSYLLFNEVEDIGAMFSKIDQITANDLMDAAN
                     EVLYPEKLSTLIYS"
     gene            24251..24892
                     /locus_tag="PLK75_04115"
     CDS             24251..24892
                     /locus_tag="PLK75_04115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOY38462.1"
                     /translation="MSILPEELENYLETFCDAEDQILYDLSRETHLKTVHPRMLSGNL
                     QGKLLTMLCQMIKPVNALEVGTFTGYSAICIARGLEKKAMLHTIEINEELEPICKKYF
                     ERAGLNNKINFIVGDALQIIPRMDMIFDIVYLDSEKSTYPDLFVLLKDRISKGGYLIT
                     DNVLWDGKTGHANGARDKMTQGIIRFNQLIREDKSFEKIVIPIRDGIMLARKN"
     gene            complement(24899..25294)
                     /locus_tag="PLK75_04120"
     CDS             complement(24899..25294)
                     /locus_tag="PLK75_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38463.1"
                     /translation="MKNIFAVLVTVAILFGSISVMAQSKTLQGGSSDQNNPPKEQEQT
                     VVKNTNCEKTVVKYEKFATRYLKIARTFELNPKNKALINSAAKLTSQAATWGTRISSL
                     TSCGDTYQQRIKAAQKKVNEASKIIQKLQ"
     gene            25583..26368
                     /locus_tag="PLK75_04125"
     CDS             25583..26368
                     /locus_tag="PLK75_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOY38464.1"
                     /translation="MSKLLSLLKSKLLWINVGLAVAITIVLAWIVLFFLRIYTDHGES
                     VTVPDLVGLSMKEAEELCDDNNFQFVITDSVFSDEVDKGAVAEQNPVAGFKVKEGRTI
                     YLIVNSTSDETVQMPTLEGVSLRQAHALLETYGLVAGNLKYVPDIAENVVIRQLYKGR
                     EIEPGTKIKKGSRIDLVLGIGLSDERATVPNLINMSYNQAYNKLLDEFLNVGAVVYDK
                     SIRTKRDSAEARVYRQSPMPDTINTINLGSNVDIWLSRDTAAF"
     gene            26394..28244
                     /locus_tag="PLK75_04130"
     CDS             26394..28244
                     /locus_tag="PLK75_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38465.1"
                     /translation="MKKCFLILTSFAFSLIAFTQEVLIDVVPSSLVTYSRTYFSGMNT
                     KETMDSDTLELPFYDDFANSVVFPSSDLWIDKNAFINDGYAKDPISIGVATLDVLDSV
                     GAVYSFLSSTVSSAAECLTSRPINLNYTAADSVYLSFYYQCGGYGNKPDVGDSLVLEF
                     TTPDTTWTSVWQADGGVAMDSFRLVLIPITQEWLLKKGFQFRFKNHASISSNYEPSWI
                     SNADCWNIDYIRLDTARNQNDTITQDVAFISYFSSMIKDFESAPWNHFEPIASLNTID
                     SLVYVYKNNGPTTQNINRQLLIKDVWGNGTGFSNLDDSENILPFSTLSYAKNVNYTFN
                     SGTVDSARFELKGFIKTDTTLARRPFRWNDTICYYQTFYNYYSYDDGSAEKGYGITGQ
                     GTTGSAVALQFTPLQNDTLRGAYIFFNQVLDEGNIHYFFFTVWDDEEGVPGDTLAKQL
                     GVRPVYSDSINEFVYYALDTPLLITGTFYIGWIKTTDDMLNVGFDVNRDASQHLFYNT
                     QGDWISSSLPGAIMVRPVFGVNPDPSWVKPQNTDNYTLFPNPATNAISIRNTNGQSPD
                     KCAIYDISGRLCLESYNTEFVNIHSLPEGLYFIRFADRGNIRTLKFVVSR"
     gene            28246..29298
                     /locus_tag="PLK75_04135"
     CDS             28246..29298
                     /locus_tag="PLK75_04135"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOY38466.1"
                     /translation="MSEDSTSLSNQEDYKNQEELFEHYRFIVDNGQSPVRIDKYLASK
                     IANVSRNKIQAASDAGNIRVNDTPIKSNYKVKPADVITILLSFPKIEFELIAEDIPIE
                     VVYEDNDVLVVNKRAGMVVHPSLGHYQGTLVNALAFHLQGNELFSENNMRSGLVHRID
                     KDTSGLLVIGKNERALNHLGKQFFEHTARRTYHALVWGNTAEEKGTITGHIGRHLKDR
                     KIMDVFEDGSYGKHAVTHYQVLDRFGYVTYIECKLETGRTHQIRAHFSHIGHPLFNDS
                     DYGGNQILKGPIFNKYKQFVSNCFEICPRQALHAKTLGFIHPVTKKEMMFDSLIPKDM
                     QACIDKWRSYSGSFSV"
     gene            complement(29295..29777)
                     /locus_tag="PLK75_04140"
     CDS             complement(29295..29777)
                     /locus_tag="PLK75_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38467.1"
                     /translation="METKKTLFICVAMLFISVDVFSQHTNKVKDEEKIKAEKVAFLTT
                     QLDLTVAEAQKFWPLYNEYEKKNDEIFKSERELNRDIKEKSASMTEAELEQKLDKMMQ
                     LQKSKSDLSVEYYAKFKSVIPIKKVAKFYQAEREFRKQLLHEYGKCYGQGGTEPGGPH
                     "
     gene            complement(29782..30198)
                     /locus_tag="PLK75_04145"
     CDS             complement(29782..30198)
                     /locus_tag="PLK75_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38468.1"
                     /translation="MGINGNYINKTAFKVPEGYFDDLPDNIMSKVKRDHKPRVRTIDI
                     IKPWLYMAASFIVLAIFIKIGLNLVVDKKSVNPDTSKTENFTAYNDFEESLLSDELVF
                     MEAITETPQEAGISEVSSEAIEEYLLNYDLTAELEE"
     gene            complement(30233..30781)
                     /locus_tag="PLK75_04150"
     CDS             complement(30233..30781)
                     /locus_tag="PLK75_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY38469.1"
                     /translation="MSPEKEQEIIRCFVENKDVESAFRTIVVEYGPRLYSHIRKMLLL
                     HHDTDDVLQETFIKAYHNLSNFRHDSKIFTWLYRIATNETLNFLKKKKRQHVFSDFDY
                     ENYMINSLKEDPYFDGDELQINIQKAIMSLPEKQRLVFNMRYYDDLPYHKISEILGTS
                     EGALKASYHHAVKKLEKTLNLT"
BASE COUNT         9463 a         6163 c         6109 g         9369 t
ORIGIN      
        1 ccgacctcaa caacaaccct catgaggaat ctggtcttat ttatctggtg aaatagaatt
       61 caatattgta tctttaactt ttttaaataa tacttgccat ataaaatacg ttattaatcg
      121 gaataattga tactatggag aaattcgcac attataacgt attcattatc ataacattaa
      181 ttacattttt tgccctcagt atgggttgtg aaaagaataa cttcacggaa aatcccgatg
      241 acaaattgat tttttcggaa gacactattc agttcgacac aattttcaaa tcgattggtt
      301 caacaacaca tttttttacg gtttacaatt caaatgaatc aaaatccatt aatattgacc
      361 gcatttacct ggcaaaaggt aataattcaa gctttcgtct gaatgtaaat ggaattcaat
      421 ccagctcagt taccaacatg gttatagctc ccggcgattc tgcatttatt tttgttgaag
      481 taactattga ccctggcagg gatgaaatga ttgagcaaga ctccgttgtt tttttatcaa
      541 acggaaacat tcaggatgtt gacttaattg cttttggaca ggacatccta ttaattaacg
      601 gacagatatt ttcatcagac actgtctgga ccaataataa acctgtacta atcttcaatt
      661 cagctttatt cgactcaaac acaaccctaa caatagaagc agggacaaaa gtacattttc
      721 ataagaactc aagccttttt atcaaaggga gccttcttgt gcacggtgca aaagaaaatc
      781 atgttgtttt ctcaggcgac cgacttgaag atgaatattc ggagatatca ggacaatggg
      841 gtgcttatca gactgacgac gttggcaatg ttactgcact ttatggagga atccacttgt
      901 tgcagggttc tgcaaacaac attattgaat atgcagaaat taaaaacgga attattggca
      961 tccgggtcga ttcggttaca acgccaggga caccaagcct gatcctgcgg aatacttcga
     1021 tagaaaacat gaatattgcg ggcttatacg gattgggctc acacattgaa gcctacaact
     1081 gtgtttttgc aaattgtggg aaatacactg tagtgtgtgc aattggtggt aattattcgt
     1141 tttatcactg cactctcgca aatttttggt ccggaagcag gcagacccct caattgctgc
     1201 tgaataatta ctataactat agctctcatg gcagtacaat taccgaatac agagatctta
     1261 ccaatgcata tttcgggaat tgtatcattt atggaaatcg tgagcaggaa atttctctgg
     1321 atcttgaaac aggagccgag gctaacttta ttttcgaaaa ctgccttctt aagacatcat
     1381 ccacttttaa tacaagcaat acatcgtatt tcatcgataa tatccttaat gtaaatccca
     1441 agtttattga gactgtatca ccttataatt atgagctcga cacgttgtcc cctgcaaagg
     1501 atgcaggcaa attgtcggtt tctctcttaa ttcctttcga ttatctgggg aacaatcgat
     1561 tgtcggatgc aggtcccgat ttgggagcat ttgaaagaat agaatgagta aacttttgtt
     1621 tgtaatatcg attgtcattt caattgccgt aatttcatgt agaaacggca caaaggagat
     1681 tgctgtaagc gatgcgtaca atgagttcag taaaacagaa gcagacaggt ttcgatttat
     1741 gttctataat gttgaaaacc tatttgacat ttataatgat tcattgaaaa acgatgatga
     1801 atttttgcct gatcaggcaa aatactggac cgccagtaaa tattacacaa aactcaatca
     1861 tatctatcag gttattgttg ctgttgggga gtgggaactc cctgatataa tcggcctttc
     1921 agaaatcgaa aaccgcattg ttcttgaaca actcattgaa aaaacgccac tttataaaac
     1981 gaaatataaa ataattcatt atgagtcgcc tgatcccaga ggcattgatg ttgcattatt
     2041 gtataaacca tcttcattca aacccctata ttctgaaccc ataagaattg ttttccctgg
     2101 cagtacctct ggtggaacgc gagatatact ctatgtcaag gggataatta acaacaacga
     2161 cactttgcat atattcatta accactggcc atcacgttgg ggagggcaga tggaaaccga
     2221 agataaaaga atgcattgtg caactgtagt tcatcataaa accgactcaa tactcaaagc
     2281 aaatcccaat tcaaacataa taatttccgg tgatctgaac gactacccaa ccgacaaaag
     2341 ccttcaggaa tgccttaaag cacaaagcag tctcgaaaaa atagaaaata aaaagttata
     2401 taatctatcc tattaccttc aggagaccaa acacttagga acccataagc acgatggtca
     2461 ctggggagtt ctcgatcaga taatcgtttc gggggcatta cttaatggca caggtggact
     2521 tatcacctct actgaaaata tttttattct tgacaagcca tttattttgg aagaagatga
     2581 aaagaacaca gggttgcaac cgttcagaac ctatgctggc tacaaatacc tcgggggctt
     2641 ttccgaccat cttcctacat atattgattt aaaaaaaaat aagccacaat aaactgcggc
     2701 ttattataag tgatgtaaaa catcttaaaa cttaactcca atagtggcta atattctgtt
     2761 gctgttctta gaaatatcag aaaccgggct ggttaatacg ggatcagaac tctcgtaaag
     2821 gaggtatcct tcgttaccgg ttatgtaagc atacgcaaca tcgaaataca gtgattttcc
     2881 tctgaaaccc agtcctccgg aatacatcat ctggtaagag ccttcgttaa tatgaccctt
     2941 aacgtatggg cttccaaaat atgctgcacc tgctcgaagg ctcaactgac ccaagcggta
     3001 ttccagtcca cctttaatat ttcctgtact tttgtactct gtctgaattg cctgattctc
     3061 atcaataaaa gtgtaatcgt atgcacgaag tcttgccagt gagtaatcta caaactcgta
     3121 ctcgaaacta atcagcccat atttttgaat aacgaaagcc aagctgccaa ttgctttgaa
     3181 tggagatgtc agttgataat cgtaatatcc tgcacttgag taccaggttc tgctataatc
     3241 gggatttacg aaatttgagg taatctcagt cgagtattca tcactcagat tataaaatgt
     3301 aggtgaatga attgccgctc caattcttac ccaatcaacc ggccttgcca acagtccaaa
     3361 tttaaaatta agtccagtac cgtgagtgta caaatgctct gtatagttaa atgagttaaa
     3421 atcagcaatt gcatcatcgc tatccacttc ggaatgaaag atagtttgct cgaatcgtaa
     3481 aggctgaata ccaaaggttg cacccaaata cagtaaatgg ttaaaatttg cagacaacga
     3541 aatatcaaat tctccaagat atccgtcacg agatattgca tgtgaagtat tttgtccata
     3601 tactccgtta taaacactaa tgtagttcat tgtatcggta agcgaaacct gattgatgag
     3661 gtaagtttcc catgcaagcg aagagtagaa gttatcgaca ttctgaagct gcgaaaaatg
     3721 cgttccgttt gcacgatttg caaaccagtc cgtcattgag ttattggtgt tatcgccatt
     3781 aattaaagta ttttgatgaa aattattcaa cctggtgtat ccgattccaa aattgatatt
     3841 tttcaaactt gagctttctt tattaaattt cgatgtgcca acatagccaa aactgttgat
     3901 gttgaaattg tagtcaaagt cggtagtggt tgatgacatg aagcttgctt cggaattaat
     3961 atattcgaaa gaaggggtaa ttgaaaaatc ggattgctga aaaacagcca tcccagcagg
     4021 gttggtactc aaacaggtaa tatctcctcc caatgctcca aatgcacctc ctaaagacgt
     4081 gtatctggca gtacctcccg taacaagata ggaatatctc aaggcatcaa cttcgttctg
     4141 agcaataagc atttgaaaag aaacacttaa tgctgcaaca aaaaagatta tttttttcat
     4201 attgtttaag tttattaatt aaaatattat ctacgaccgc ctgaagaatg acttccaccc
     4261 gaagaatggc ttcccccact gcttccgcct gatgaatgac tgctgccact gctgcttgac
     4321 ctaccggaag aactacccga tgaacttcct gaagaactgc ttgagcggtt gtatgaaccg
     4381 gagctacttc ctgaagagga acctctgttg taagatccgg aattacttcc tgaagaagag
     4441 ggtgtgcgat tgtatgagcc ggaattgctt cccgaagacg gagttcggtt gtaagttcct
     4501 gagttgcttc cagaagacgg tgtgcggtta tatgtccctg aaccggattt tggcgggttt
     4561 gtagatcttg aatccggtgt agtaaccttg ttagtatttc ctgagtttgc aggaggtgtg
     4621 ctaggtttgg taggagaagt ataggagtta ttccgcgggc ttgatccact ttgtgaacca
     4681 gatggattgt tgggctttac ctgattctga ttgctgttgc ccgaattatt attataattg
     4741 ggtttctctg aaggacggct tggttggttg tatgaattat tgtttggccg ttctgcaggt
     4801 ttagtaggct gattgccaac attattgggc ctatcagcag gttttgtggg ttgactgtta
     4861 ttataacccg gtttctcggc tggggttgta ggtcttgtaa cttggttgtt gtcaggccta
     4921 tcagcaggct ttgagggttg attaattcca ttgtttggtt tatcaacggg ttttgtaggt
     4981 tgattgttgt tataccccgg tttctcagaa gggtttgtag gtcttgtaac ctgattgtta
     5041 tcaggcttgt caacaggttt tgtctgtgga gtttcagcag gtttgttgtt tattggttta
     5101 tctgttggtt tatcaaccgg cttattgttt actgcctcat tatcattggc tttaacaggg
     5161 cggtcgaaat ttccagaagg tttttcattt gcattcgggt caacttcacg gcccagattc
     5221 gtagcattat tcgtattcga atttatattc atcggagtat tcaggttatc ggtgttttca
     5281 ggcaaacggt ttgatgaaga agaaccgttc ccacctaccg agccccgatg cccatagtac
     5341 acattcgagc cataatcgct ccagtcgttg taatcgttgt acacatagtt atcgtggtag
     5401 ccatcgtagt atccatccca gtagccatga ctataaccat tccagtaagg gcttccccat
     5461 ccccagtaat accctgacca actatatggg taccaccatg accagtagta cgggtaatat
     5521 gagtaacccc atccataata ccacgaaaaa ccccaattcc aatcgtaata atatggatcg
     5581 tagtgataaa cataatatgg attcagtctg taataataac tgtcgtaata atcatcgtaa
     5641 tagtagtaat tattgattat tgtacttcca tcgtcgtaat atgtatcgga atacgagttg
     5701 ccatcgggat agtcgtaatt ataataatcg gaactctcgg aataattctc tccctggttg
     5761 taatactcgt ttatcgtggt atcttctgaa taagtattgc cagaatcgaa ttcttcctgg
     5821 taaactgttt catacacagc tttgtcttcg tcaaccgagt tgtcggttac agcgttctgt
     5881 gggttattat tattgtcgct ttttgtcata tatacatcgt cgtattcacc tccaactgag
     5941 gtgtagaacg atgttgtgca agatgtggcg actaacagaa gcacaaaaac gaataaaagt
     6001 acatgtgttt tcatgataac tttatttttt atagatttgg cactttgtaa catgattcaa
     6061 tttttctgaa aacgtaacaa aatttgtacc aaaaatggca aaagaactga cttccagagc
     6121 agaaaattac tctcaatggt ataacgacat tgttgttaaa gcagaccttg cggaaaactc
     6181 agcagtaaga gggtgtatgg ttattaaacc ctatggatac tcaatatggg aaaaaatgca
     6241 atctgcgctc gatacgatgt ttaaagaaac ggggcatcaa aatgcatatt ttcctttatt
     6301 catacccaaa tcgttttttg ccaaagaggc aaaacatgta gagggctttg ccaaagaatg
     6361 tgcaattgtt actcactatc gtttaaaaaa tgatgaaacc ggcaatcttg tgattgaccc
     6421 tgaagccaag ctggaagagg aactgataat acggccaaca tcagaaacaa taatttggaa
     6481 tacctatcgc aactggatac agtcgtaccg tgatttaccc ttgcttgtta accaatgggc
     6541 taatgttgtg cgatgggaaa tgagaacccg cttgtttctt cgaactgccg aattcctgtg
     6601 gcaggaaggg catacggcac atgcaacaca agaagaggcc attgatgaaa ctaaaaaaat
     6661 gattggtgtt tatgccgatt tcgcaaaaaa ctggatggca ttaccggtcc acattggcac
     6721 gaaatcggat aacgagagat ttgcaggagc tatcgaaact tttgcaattg aagccctcat
     6781 gcaggacgga aaagcgcttc aggcaggaac ttctcatttt ttaggtcaga attttgcaag
     6841 agcttttgat gtgaagttct tgaataaaga caataaagaa gaatttgtat gggcaacctc
     6901 atggggcgtt tcaacacggc tgctgggggc actaataatg gcacattccg atgacaacgg
     6961 actgatcctg cctccaaaac tggctccttt gcaagtggtg attgtaccta tttataagaa
     7021 caacgaacag ttggagcaaa ttggtgtaac agcgctaaat attgcagaaa aaatcagaaa
     7081 aacaggcatt agcgttaaat acgacgatcg tgacaacctg cgtcctggct ttaagttctc
     7141 ggaatacgaa cttaagggta tccccgtaag gatagcaatt ggtccgaagg atatggagaa
     7201 taatagttgt gaaatttgtc gtcgcgatac tcttgaaaaa tacaatattt cgcaagatga
     7261 tgtaataagt catattcccg agctattgga gacgattcaa gaggaaatat acaacaaagc
     7321 gattgcccga agagatgcaa acacttatta tgctgaaaca tacgaagaat ttaaagaaat
     7381 actcgaaaac aaaccgggtt ttatctgggc acactgggat ggaactaccg aaacagaatt
     7441 gaagatacag gaagaaacaa aagctacttt gcgctgtatt cctgtgaacg gggataaaga
     7501 agaagggcgc tgcattgtaa ctggaaaacc ctcaaaacaa cgggtgttat ttgcaaaatc
     7561 atattaaaaa cacgaattat gaacaatagt cagaatataa aagcacaaat tgttgctaca
     7621 ctgaaagaaa aatcttcaac aaaacaaatt gtttacgatc aaacaaaagc agcttttaaa
     7681 atcattaaaa gtattttgca aaatatagta gatgaatata atgaagcact taaaggatca
     7741 gatgagcgta taatgcttga gtaccgcgac aggggcatgt ttgaggccga gattaaggtg
     7801 gccggtgatt tacttatttt taatatgcat tccaatattt ttgagtttga ctataatcat
     7861 cccatttggc gcaatccgga ggcaaagaat gagcccataa aaacatattc tgggataatt
     7921 aacatctata attttcttgc agactctttt aaatatcaaa gactggaaga ccttgggtac
     7981 ctggttggtc gaatgtttgt gaacatggaa ggcagttttt ttgttgaagg cagacgtgag
     8041 ttaggatttt actacccggc ttttggtaaa aacatggttt cttacgaaaa cctaaggatg
     8101 atgattgagt ccactataat ttatgcactg aaattcgatt tacttgttcc cccttacgaa
     8161 gacgtaaaaa tttcaacggt cgaagtaatg aaagaaaaaa ttaacaaatc gaaagttaaa
     8221 actggtaagc ggatgggatt tgcttataat gtggatttag gcaaagcgac aaccgttgac
     8281 acttatacag acagtgaatg attttgcaat taatcgtatt gcaaaataaa tactaactag
     8341 aacacaatga ggcaggaaat aaaatatgaa agcaaatctg catattatgt tttaaaatag
     8401 ttttattata aatatttgat ttactaacac ttacctcatt tataaagata taaaaaaata
     8461 catattcagg tactccattc atataaagta ttaaatttgc cccaacaaac aaaacaatat
     8521 tacaatggct aaaaatcata gtgttgtaca ggctgaatct gttgtcgtaa aatttgccgg
     8581 tgattcaggg gatggtatgc agctggcagg gaccatgtat gctgacattt ctgccctgat
     8641 tggcaacgat ttagcgactt ttccctatta tccggctgaa atacgcccac ctcaggatac
     8701 cgttgccggt gtttcaggat ttcaggtaaa taaaggcgcc aacataaaaa cttccggtga
     8761 tgaatgcgac atactggtat gcatgaaccc tgcatctctg gccgcaaact tcaagtttgc
     8821 taaaaaaggt ggaaccatta ttatcgactc cgatagcatc actccaaaag cgcttgaaaa
     8881 agcaggctac aaatcgaatc cgcttgaaga tggaagttta agtagtttta caatcattaa
     8941 agctcccatg acttctattt cggtagatgc ttgtcagcac cttgatatag acaacaaatc
     9001 agctgcccgt acgaaaaaca tgtttgcatt gggcatggtt gtttatattt gcgactacga
     9061 tctggaaaag tcgttttatg taatcaataa gaaatttaaa gggaacccga cacttgcatt
     9121 ggcgaataaa gcagctgcca aagctggata tattttcgct gaagccagtg agattaattt
     9181 ctcgaggatt cacactcctg taacaaaaat cgaaaaaggg agatatcgca ccattaccgg
     9241 aaatattgcc acagcatggg gcttgcttgc agcttcagaa aaatcagggc gtcagctgtt
     9301 tttaggctct tatccaatta ctcctgcaac agaaattctg atggagctgg caaaacacaa
     9361 atcgctggga gccaaggtat ttcaagcaga agacgaaatt ggaggaattt gctcggcaat
     9421 tggggcttct tatgcaggtg cgctcgcatg taccactaca tccggacctg gtctttctct
     9481 taaaaccgaa gcccttgggt tggctgtaat agcagaatta ccgctggtcg ttgtagatgt
     9541 acagcgagga ggcccatcaa ccggattgcc taccaaatcg gaacaatccg atttatatat
     9601 ggcattgttt ggaagaaatg gagaagcacc actattcatt ttggcagcat caagcccgtc
     9661 cgattgcttt ataaaagcat ttgaagccgc taagttttcg atggaacata tgacgcctgc
     9721 tatactgctc actgatggcc aaatgggtca gggatccgaa attttcaaga tccccaaaat
     9781 ggcagaactt cctgctatta agccgcccat tgttccagca aatgataaag attacaaacc
     9841 atatctcaga aatcctgaaa cactagctcg cggttgggcg attccgggca cagaaggatt
     9901 gcgtcaccgc attggaggcc ttgagaagac aaatgtaacg ggaaatgtgt cgactgatcc
     9961 aatgaatcat gaattaatgg ttaaactcag agctgataaa gtaacaaaga ttgctgacta
    10021 tatccccgag caaactatcg attatgcaaa atcaggcgat ttgctggtgg ttagctgggg
    10081 cggtaccaaa ggttcggtat caacagccgt tggggagcta ttcgctcagg gaatgaaagt
    10141 tgggcatgcg catttcagtt atatatcacc cttaccaaag aatacgggca aagtacttgg
    10201 caaattcaag aaaataatag tctgcgagct caatgccggt caatttgtta attacttacg
    10261 gatgaatttc ccgaaacacg attacctgca atacaacaaa attcagggat taccttttac
    10321 aacattagag cttaaagaac aattcacaaa acttttatag gaggtcatta tgagctttga
    10381 aaacaatttc acaatagaca gaagtgaagt tccattaact aaagaggact tcgtcagcga
    10441 ccagatggta aagtggtgtc cgggatgcgg cgaccatgca attttggcct cactcgaaaa
    10501 agtgtttcct gaaataggat acaagaaaga aaatttctgc attgtttcgg gtattggatg
    10561 ttcatcgaga ttcccgtatt atgtaaacac atacggattt catgggattc atgggcgcgc
    10621 taacgttata gcatcaggtg taaaaattgc aaaccctcat cttagtgttt ggattgccac
    10681 gggagatggc gactcaatgg caattggggg gaatcatttc atacacatta tccggcgcaa
    10741 catcgacgta aatattatgt tgttcaacaa ccagatttac ggattaacga aggggcaata
    10801 ttctcccaca tcgcctatgg gttcaaaaac aaaaacatca cctcagggaa caattgaaca
    10861 accatttaac ccgggagaat tggttatggg tgctcaggga actttctttg ccagaacaat
    10921 cgacacaaac cccaaacaca tgactgaagt aatgttcgaa gctgcccgtc acgatggaac
    10981 ttcgatagtg gagatcctgc agaactgtgt gattttcaac gacagtaccc ataagctgat
    11041 taccggacgt gatgttagag atgataatca gttgttgttg cagcacggtc agcccatgat
    11101 ctttgggaaa gacagaaaca agggtataat tttgggtaaa aatgggttgc aggttgtaac
    11161 aatcggtgaa aaaggaatta aagaaaagga tatccttgtt cacgatatgc atcagaaaga
    11221 tcctggtatt catttgatgt tggcgaaact aaagcctcca acatatccga tagccttggg
    11281 tgtaattaga gctgcaagct ttcccacata cgatgatttg gtggaagaac aaattagtta
    11341 cgcaaaagac actgcccaaa ttaaaaatgt cgacgattta ctgaattccg gcgacacatg
    11401 ggaaatataa aaaaagccct gacacaagtc agggcttttt tttgtcttcg tcatttgaga
    11461 tgctcagtaa tcgggtctgt tgatctatta taaaacaagt cccttcttcg gccagaaaaa
    11521 ctggttggaa tacttgaaga gcttcatgtc ttaaaaattc aacactggga taccttgtgg
    11581 aataatgtcc aattatcagt gccttggcat tcgtgcattt tgcaaccata gcagcctgtg
    11641 aggcagtggc atgaaaagtt gcttttgcca aagcttcagc atcagaggca aatgtagctt
    11701 catgatacaa tacatctact tcaccaactc tatcatgcaa gtttttgata tatagtgtgt
    11761 cggaacagta tgtgtatgta cgaggctggg gggaatcaaa aaccaattct tcatttggaa
    11821 taatggttcc gtcaaataaa acaagatcgt ttccctgttt tatttcatgg atctcagcaa
    11881 ttccgggctt atacatttct attgcctcct ttttaatttt aggtgggaat tgcttttcgc
    11941 aaaatttaaa tccccagttt gctttgctgt gttccaaagg aaaggaatag accattaggt
    12001 agcgggtttc cactaaaagc acaaagtccg gagaattcaa tgggtgaaat tttacttcga
    12061 aaagaagcgc tgtagctgtt aatcgccaga ttgaattcag aattttatga agttccgggg
    12121 gagcatacaa gtgtagagct ttcttccttc caagcatatt catacttgta aggagtccaa
    12181 aaataccaaa gtagtggtcg ccgtgaaggt gtgagataaa tatatgttcg atctgtagaa
    12241 agctgatacc gtatttacgt aattgtattt gcgttccttc tccacaatcg accagcattg
    12301 gagtattatt gtagctcaca acctgtgccg atgggaaacg cttcgaggtt ggcaaggcac
    12361 tggagcttcc cagtatttgg atttcgaatt tcattgcggg aacaatgtaa aaaaatgccg
    12421 gtattaaccg gcatttttcc tattattctt tcgatttttg cttcttggct agttccttct
    12481 ttatttcatc aagatcaacc atatcaccta aggtgttttt ttcaatttta tcggaagcag
    12541 gtttggtttt gcgtggagct gttcccgact ctttttgttt tgccttacgt tcttcctcag
    12601 ctttttcacg tttttcgtct tcatgaatcc tgctatgaga aacgaagatc tttttagcat
    12661 ctttattgaa ctcaatcact ttaaagttga ttctttcgtc aacttttgca ggggtaccat
    12721 cttccttcac cagatgtttt ggggttgcaa aaccttcaac gccatatggg agtgcaatga
    12781 tagagccttt gtcgttcatt tcaacaatag taccttcgtg tattgaatcg agtgagaaaa
    12841 ttgtttcgaa aacatcccat ggattttcct caacctgttt atgcccaagg ctcaggcggc
    12901 ggttttcctt gtcaatttca agaacaacaa cttcgagatc agcaccgatg cttgtaaact
    12961 cagcaggatg tttaattttc tttgtccacg acaaatctga aatatgaaca agtccatcca
    13021 caccttcctc gagttcaaca aatacgccga agttggtaaa gttacgaact ttggaagcat
    13081 gtttcgatcc gattgggtat ttggtttcga ttttctccca tgggtctggg aatagctgtt
    13141 taatacccag cgacattttg cgttcgtcgc ggtcgagagt tagaacaaca gcttcaacct
    13201 catctcctac tttcataaac tcctgtgcgc tgcgaaggtg ctgcgaccat gacatttccg
    13261 aaacatgaat aaggccttca acaccggttg atatttctac aaatgcacca taatcggcaa
    13321 ttaccacaac tttacctttt actttatcac caactttaat gttcgcatca agagaatccc
    13381 acggatgctg tgtaagctgt ttcaagccaa gtgcaatacg ttttttatcg tcgtcgaagt
    13441 cgagaattac cacgtttatt ttctcatcca gggtaacaat ttcttcggga tgattaaccc
    13501 taccccagct aagatcggta atgtgaatga gaccatcaac accaccaaga tcgataaata
    13561 caccatagct agtgatattc tttacagtac cttcgagaac ctggcctttt tcgagtttgg
    13621 aaataataag ctttttctgt tcttcaagtt ctgcttcaat aagagcttta tgtgatacaa
    13681 ctacgttttt gaattcatta ttaattttca caactttgaa ttccattact ttgtcgacat
    13741 atacatcgta gtcgcgtatt ggtttcacgt cgatctgaga ccctggcagg aatgcttcga
    13801 tcccaaatac atctactatc ataccgcctt ttgtacggca tttaatgtaa cctttaataa
    13861 tttcatcgtt atcatgagct ttgttcacac gttcccagga acggagtgct ttggctttgt
    13921 tatgtgaaag taacaactgt ccgttgccgt cttccatatt ttcgacatac acttccactt
    13981 tatcgccgat tttaaggtcg ggtgtatagc ggaattcatt agcattaata acgccttccg
    14041 atttgtagcc gatgtttaca accacttcgc gtttattcat tgcaacaacg gtgccgtcga
    14101 aaacttcgcc ttcgtgcacg gttgataaag tgtgattgta gagttcttcc aactcactgc
    14161 gctgtttcag ggtgtatgat tcatctcttt tacccagcac ttcccagttg aaatcatcta
    14221 aaggtttaat ttcttttgtg tcaaaaacaa aaccttttgt ggattttttt gtttttggca
    14281 tttcggcaac cggttctaat acctctgctt taacctcctc tgcaacaggg gtttcaacat
    14341 tttcttctgc agtttgctga ctggttgcta aagtttcgtt ttgattttga tccaaacttt
    14401 cgtcctgaat cagattgttt tcgttttctg tcataattgt aattagttca gattaataag
    14461 ttagacatta tttatagtta gaattcgagc cgcaaaagta gtgttttttt cttatttgca
    14521 aagcaattag actattttat tttcaagggt aaccccaatg gtgaattgtg gatggagttt
    14581 ctctcagaga acgttggatt tcaagttttt ctcaatggct atttaccata acgatgccct
    14641 ttgagagcaa aaaacataat aaacgcatag caaggaataa ttattaggta ggcatagtgc
    14701 gttcctatgg gatgcagatc actaagtgca ccatacaata aagggattgt tgcaccacca
    14761 actataccca taatcaggag cgctgagcct gtttttgtat attttcccaa accttcgatt
    14821 gaaagaggcc atatggcagg ccacatcaca gcattggcaa gtcccagtaa cgcaataaag
    14881 ataacagagg ttatcccgga tgtaaccaca gcaagcaccg ttagcactaa agcaagtgcg
    14941 gttgcataga aaagagcttt tgattgcgaa ataagtcgcg gaatagctgc aataccaatc
    15001 aaatagccgg ctaccatggc aaaaagagta aaagaagcaa aaaaacgaga cgtttcgagc
    15061 ttaattccta ttgatttacc gtaattaata atagtatcaa ccgatatcac ttcggcacca
    15121 acataaagga aaattgacaa tacacccagc cacaaatagg gatatttaaa tattgatgat
    15181 cgtgaaattc cactcttatc agaatctccg gatttgtcaa tttcgggcag tttacttgcc
    15241 ttaaccagaa gtgccagaat cagtaaaact actgcaatga caatgtaagg tactattacc
    15301 cgctgcgaaa gtaaatccaa ttcagcagtc cgctgagcaa gactcatggt tgtaatttta
    15361 tcggtgatag tgtcggcatc tttcaataca atggttccta aaatcagtgg actgataatt
    15421 cctgcaattt tatttgcaat gcccattata ctgattcgtt ttgcagcgct ttcaatgggt
    15481 ccaataattg tgacatacgg atttgatgcc gtctgaagta ttgaaaggcc tgctccctgt
    15541 acaaacaatc ctgtaaggaa caatggataa aatcgcatca tcgccgccgg aataaaaatg
    15601 atcgagccaa tcgacataac tattaaaccg agcgacattc catttttaaa accggtttta
    15661 cccagcaccc atgacgatgg caatgctaaa acaaaataag aaacataaaa ggcaaatgca
    15721 acaaataaag actcgaggtt tgtaagctca cacgctattt tcagataggg aatcaaagtt
    15781 gcatttaacc aggtaacaaa tccgaaaata aagaacagca tccctataat gcttattgca
    15841 taatatttcg ataccgatgt tacatcacga gccataacga agattttaat ttattgttac
    15901 aaatttggaa aagatgtttt agaaaactag gtacaaccaa aagcttttac taacaattaa
    15961 gtgctcacat gtgatttttt ttcttgagtg gtaaaaggtt atttaatcaa gtttttttaa
    16021 gcttcaatca ttgcaagaaa ttcattctca ctaattattg ccacacctag ttctactgct
    16081 ttttcgagtt ttgcaggccc catattttcg cctgcaagaa caaaatcggt ttttgatgaa
    16141 atactacctg tgttttttcc accatgcatt tcgatcagtt tcttcagctc ttcgcgcgaa
    16201 tatttgttaa ataccccgga gattacaatg cttttgccat ccaatttatg ggtaagtaca
    16261 tgttccccgg cttgttcaaa ctgcaaaccg tatgaccgaa gtctttcaat caattgccta
    16321 tgttctggtt tcgaaaagta ttcaatgaca ctttcagcaa ttttttctcc tatttcatca
    16381 acagccataa gctcttcggt gttggcactc attaaagcat cgatattacg aaaagcttcg
    16441 gcaagttttt ttgctacagt ttccccgaca aaacgaatgc ttaaaccaaa gagcaccctg
    16501 tgaaaaggta cttcaagcga ttttgcaata ctttcgataa tattatcagc cgatttctcg
    16561 gcaaagcgtt caagagggag tatatcgttg cggctaattg tgtatagatc ggcaatatca
    16621 ctgattagtc ctttgttata gaacagttct acagtttcct ccccaagcga atcaatgttc
    16681 aatgccttac gactgataaa atgaataatc tttcctttaa tctgtggggg gcattgatca
    16741 tcgttgggac agaaatgttt ggcctcaccc tcatttcgtt gcaataaata accgcactca
    16801 gggcagtgcg tgataaattc tataggtaaa gcactttcgt tttgcagtgc aatacctgtg
    16861 attttaggga taatttcccc tcctttttcc actctcacac ggtcgccaat gcgtaagccc
    16921 aaagtagaca tctgatcagc attatgcaat gttgcacgct tgacgatcgt acccgcaaga
    16981 agaatcggtt tcaggttcgc cacaggggta actgccccag tgcgcccaac ctgataatca
    17041 acactaatga gttcagtaac tgcttcttct gctttgaatt tataggcaat tgcccatcgc
    17101 ggggtcttag cagtgaagcc caataattcc tgctgttcca atgaattcac cttaattaca
    17161 attccatcta tatcgaaagg caaatttttc ctttcttggt cccaataatc gatgtaatcg
    17221 aagacgtcgc caattcctga acatttgaca atgtgttcag ggatacggaa tccccattca
    17281 cgtgcttttt taagcctggc gaaatgttca ttatatggga gctgatcact tattaaatag
    17341 tacaagtaac aatccagctt ccttcttgcc acttccgatg actgaagcag tttcactgtg
    17401 cctgcagcag cattgcgcgg atttgcaaac aaggcttcac ctgatgaact gcgctgttcg
    17461 ttgagttgat taaaaacttt gcggggcata tatacctctc ctctgatttc gaaaacctcc
    17521 ggataatcgc tgcctattaa tgtaagtgga atacttcgga ttgtacgtat gttttcggta
    17581 acatcatcgc cctgcaaacc atcgccacgg gtaatagcac gttgtaagcg gccattctca
    17641 taatgcagac tgattgaaat accatcatat ttgagctcac aaacatattc cggctgcaac
    17701 ccaagagctt taaccactct atgatgaaac gcttctacct cttcgcggga ataggtgttc
    17761 ccaagcgata acattgggct gatgtgcttt accggagtaa accggttact caggtcactt
    17821 ccaacccgct ttgcagggct attggggtcg tcaaattcag ggaaagcctg ttcgagttga
    17881 acaagctctt tcataaatac atcgaaatcg taatcgctta tctgaggctg agataacaca
    17941 taatacaaat agttatggtg ttctagttct ttccgcagat aagtaattct ttcaaaagct
    18001 ttttgtttat ccatatcacg aagttatgtt tcacaaagat tgtgaaattt tgaatttgag
    18061 cgtgactttt cggtcaaatt caactttaaa atataaacaa aataatgata ggattttaat
    18121 tatccccata gttgtttccg ccaaacctga aagtaacgaa caaaccgtag tttttcggga
    18181 aactggttaa tgaatatcca tcccaatagg gaacatttaa tgcaaggtca acaaattgat
    18241 catattctac ggcaatccac cagggtagtt gaaaataggg ctgaaactga actgaaatag
    18301 catcgcttat tggcaaatca aaaataacat agatcttatc ttccacaata accgatttat
    18361 caagccatga gtatttatca tcattaattc tggtaaactg ccggtaatta tctatgtcaa
    18421 ccgataaccc ccaccaaaga atggggaact ttttaatacg gaaatgcaca ccatacgtga
    18481 gacaattgtg cctatacttc acatcacggg ttgtgtattc attggtgttc gggtcaatac
    18541 ccccagatga aatcaccgcg tgatgattgc tccatgtaaa ttcgttactt acgttacttc
    18601 cctgaaagtg atatccaatt ccaaacactg caccatgcag taataccaca tgattcatct
    18661 gattatcgag gtatgggcgg ttttcgttgt aaatatctac aacatcgttg aactgagggg
    18721 taatgatacc tcctgcatta taaccaatac gcccgtatat ctgcccaata gcgttggaaa
    18781 aaatcagaat taacggtaag attgcacaca gtttaatatt tctcatatgg tcgggttttg
    18841 ggtaagcgac agtaataacc aaatatcgac attattctgt taaagtacaa ttaaacagct
    18901 gacattaaga ttagcatttt agaaacctga gtaagcagcc acttataata tattcattta
    18961 aggcaaaggc atgtgtaaat tcatatatta attaaagaat tttttaactt cagattcgaa
    19021 taatttttcg acatgttttc catcgcgggt tctggcaagg ccacccctcg cagtttcttt
    19081 ataatcgctg ttaattcgtt ctcccacttc ccgcatagtt agtataacct catcaggaga
    19141 tacatacgat tttacaccgg aaagagccat cagtgcagcg cttatagcta ctgaagaata
    19201 tataccgtta cgttttacac aaggcacttc aacaagtccg gcaataggat cgcaaatcaa
    19261 tcccaggcta tttttgatag ctaatgtaaa agcatttaca atttgatatg aatttccgtt
    19321 ttcgaggtaa cataaagcag atgcagccat cgctgccgca gcacccacct ctgcctgaca
    19381 cccataatcg gctccggccg tggtaacatc attaaaaaga agcataccca aaaaaccggc
    19441 aactatcaaa gagcgaagca tcgcatcttc ttcagcttgc ttaaactcgc aatacgattt
    19501 caatacacca ggcaatattc cgcaagatcc ggctgttgga caggctacga taactccact
    19561 ttttgcattc aactcattta ctgcggttgc ataagccaca gctcttccat aaatattgca
    19621 aaaaagcggg cttttatcaa taaggctata aatcttataa gcatcgtcac ccgaaagctt
    19681 cagcattgaa agttccttgc ttagaactcc atcaccggct gattgtagca taatattgaa
    19741 gtagtacttc atttgttgaa ggatgtattc ttctgagcat tgctgtaaat ttctttccac
    19801 ctgaagggct atttcatcaa tactcatact ggtttcatca gaatgggtca gcaactcttc
    19861 aaaagtatta aaaggcataa tataattact ctcaggctgc gacttaccga aaattgactg
    19921 tatcacgggt aaagttccgt tgtaattctg cacatccaca attgtatctc cgttaagata
    19981 aaaatcagtc ttaccattaa tcggttttgc cagtggtgtg ttaaagtagc gtatttcgac
    20041 atttcctccg ccaattgaaa atcctgtaat tgtatagggt tttatatcag tgttgaaaat
    20101 gatttcagca atattcatgt tattatctgc ctgatatttt acttcttcat caatttcacc
    20161 ttttaaaaat cccaaaaagt gaagtttttg tccggcgatt actattccgt tttcacaaag
    20221 aaattccgga tctccatgag aaagcatatc cgggtgatcg gtagccaaac cccaaagccc
    20281 cccagctatt gctgcaggag ttctatgtcc cactcccgtg tcgcgaaacg agcccagaag
    20341 tttaaccgat atgtctttta tggtttcacc ctgagtcagc tttgctgcaa taatgctacg
    20401 ggcaatctgt ccaatgcggt tcgcacctgc agtatgtgag cttgaaggac ccaccattga
    20461 agggcctacc aagtcgaaaa tgctcatcgg aacagtatag tgctgaatat aacgcagaaa
    20521 atccatgcgt actgattcaa tttcttcgag acaataatcc gatgcaaaat gtagtgtaat
    20581 gcaataaaat gaattcactt ttgcagatac atttttaatt ctccaaacct cagtaaaatc
    20641 tacatttatt tcatgcattt cgatggtttc ccacaaattg gaaagcattg ttttatgggc
    20701 aacattgatg catttgattt cgaatattcg attttgttgc gaatagttac atataatatg
    20761 tgtcttcaat cttcctgaat caaaaaacaa cgattcctca ttactgaaca caggcggatt
    20821 aaattccacc gagcggtcgg aaagcattat atccttataa atactcgtca acatgaatcc
    20881 ggtctgttta tttgggaata ttctgtacaa aagttcattt gcatgaagcg ttttggttgc
    20941 tattgcaatt tgctcacgca gtttcaccgt gttgtactca tggaatacaa attcttcgat
    21001 atcgcctttg taatgataca gacatgcctg gttgcctgct ttttcgaatt tgttcaaaaa
    21061 cacctcgtgt tgcctgaaat cttcagggtc agtaacagaa aaatacctct ctggcataac
    21121 tttgcggaat ttggcggcat cgttaagtcc taatttttct tgcataccgt ttcttttgtt
    21181 gttcataaga aattgtcttt aaaattccaa taaagataat caaacaaaaa gatgttttta
    21241 atgacttacg tatttcgtaa tttcgttaac tttggttaat attcaaaaac aaacgacatg
    21301 agtactgatt tttcggaact taaattcaag tacaaaattt ggcttgagac tgaaaatggt
    21361 gaaggggtgc ttggtgaagg caaatataga ttactaaaaa aaattgaaga gacaggttca
    21421 ttaaagctgg ccattgagca gttggggttg tcgtatcgaa aaacatggga caatctgcgt
    21481 agaatagaag aaatacttgg cttcgatttg attgaccgcc aaagaggtgg ggctgccggt
    21541 ggcagttctg ctcttacccc tcagggaaag cgttttattg aggtgttcga taaatttcta
    21601 aagcagcacg atcctgtaat acaagggttt ttaaaggact taaaaaatga ccttcaaaaa
    21661 accgactaat taccttttcg tttggatttt cttcatttat aacggagcga catttaaatc
    21721 attacctttg cggcaaactg gagtcatgaa aaaaatttct tttgtgttgt tactgagttc
    21781 tttttgcctt tttgccgcgg ctcaggatac aatcagggta atgacctata atttactata
    21841 ttacaatctg tataatagct attgtacaaa ttctaacaac aacgttgata ccaaggatgc
    21901 ctacctgaaa acaataattg catataccct gcccgatatc attgccttca acgaggtaaa
    21961 aggagataat gctaccaact accggcttaa aggcagtgtg ttaaactcag atggaaggaa
    22021 ctattatatc agcacggctt acccttatgg ttcgggaagt ctgaccagct ttttattttt
    22081 caacaaaaac aagttttacg aagattatga gttgtataac atcaataccg atgcagcccg
    22141 tgatttcaat atatataaac tccgagttaa aaacaccaac cctgccgtat atgtgtatgt
    22201 aatacctgcg catcttaaag ccgggtccta cgatgatgat attgctgaaa gagcccagat
    22261 gatcgactcg gtaatgagta aaattgatga aatggggggc aatgacaact tcatttttct
    22321 tggcgatttg aatatttact cgagcacaga aacggggttt cagaaactta taaacagcag
    22381 caatttcgaa tatcgtttct acgacccaat aaaccagtta ggtgcatgga acaacaatta
    22441 cagtttccgc aattaccaca cacaatctac cagcagtcag ggcgatgact gcaaggcaag
    22501 tggcggaatg gacgacaggt tcgatatcat gttggtggat gatgatatta tttccggagc
    22561 ctcaggagta aagtatatcg aagactctta tgcaacgctt ggacaggatg gccttcactt
    22621 caatgaatcg ataaacaacg gaaacaacaa cagtgttcct tcgaacgtat tgaatgcatt
    22681 ggtgggtatg agcgaccacc tgccggttta cgctgatttc gttgtaggtg cttcaaacga
    22741 tataccaatg ccttcagcaa ataaacccct tgtaacctac aataatccgg taaaggaatt
    22801 cttatatgtt caacttatgg ctacttctgc acaacagaca tctatccgga tattcgatat
    22861 caccgggaat ttgattttta ctgagcgact gtttttgtgc aactctgctg caattcaagt
    22921 caacctatcg gcattcccat cagggttgta cctgttgcat gtcgaaagtc cggaggctgt
    22981 tcagacattc aaaatagtga aacattaatc cctgattttg catacgatgg aatttaacat
    23041 tgctacactt agcaacggta tcagggtaat acatttggtt actaattcgc ctgtatctca
    23101 ctgcggtttc attatcaatg cagggacaag ggatgagaat aacgatgaga gcggaattgc
    23161 gcatcttacc gagcacatgc tttttaaagg gacacataag cgaaaagctt ttcatattct
    23221 aagccggctc gacgatgtgg gtggggaatt ggatgcctac acaaccaaag aagaaactgc
    23281 actgtatgcc agtttccttt ctgagtatta cccacgtgct atcgagttac tgaacgacat
    23341 tgtatttaat tcagtgttcc ccgcaaaaga actgcaaaaa gaaaagtttg ttatctgcga
    23401 cgaaatcaac tcttacaaag acaacccctc agagcttatt ttcgatgaat tcgagtcgtt
    23461 gcttttcgaa aatcatgaat tgggaaaatc gattctgggg actgaaaata cagttaaatc
    23521 gattcaagtc agtcagatag aaaaatatat caaaaggtgt ttcagcaccg ataatattgt
    23581 tttcaccagt gtgggaaata tcagcttcaa aaaacttcta tctctttgcc agaatacgct
    23641 tcaaacaaac actaaaagta cccgaaattc aaaaagagaa aaatttattc agagtaaaag
    23701 gtttaccata actcattctt tcaacacgta tcaagcacat tgccttatgg gaaatacagc
    23761 ttactcatat tgcaatgata agcgccttgc cctaatagtg ttatcaaata ttttggcggg
    23821 cccagcaatg aattcccggt tgaatttatt attacgcgaa aaacatggca tcacctataa
    23881 tatcgagtca gtatatactc cttataccga aacaggtagc tttatgctgt attatggtac
    23941 cgatgaaaaa aatatttcga aatgccggca tttaatcgaa aaagaaatca gtacgctttg
    24001 taacaatcgg cttggaacat tgcaattatc gaaagcaaag aaacaaatca tgggtcagtt
    24061 ggcacttgga ttcgaaatac actcaaacct aatgcttagc attggtaaaa gctatctttt
    24121 attcaacgag gttgaggata ttggcgcaat gttctctaaa atcgatcaga ttaccgcaaa
    24181 cgatttaatg gacgcagcca acgaagtgct ttaccccgaa aaattatcaa ccctgattta
    24241 tagctaagcc atgtctattt tacctgaaga acttgagaac tatcttgaaa ctttttgcga
    24301 tgcagaagat cagatccttt acgacttatc gagggaaaca catttaaaaa ccgtgcaccc
    24361 acgaatgctt tcaggcaatc ttcagggaaa attactgacc atgctctgcc aaatgattaa
    24421 gccggtaaat gcactcgaag ttggcacctt cacgggctat tcggctattt gtattgctcg
    24481 cggattggaa aaaaaagcga tgcttcatac tatagagatt aatgaagaat tagagccgat
    24541 atgtaaaaaa tactttgagc gggctggtct aaacaataaa ataaacttta tagtgggcga
    24601 tgcgcttcag atcattcccc gaatggacat gatattcgat atagtatacc tcgattccga
    24661 aaaatcaact taccctgact tgtttgtttt acttaaagat cgtattagta aaggtggata
    24721 ccttataacc gacaatgtac tttgggatgg aaaaacagga cacgctaatg gtgcaagaga
    24781 taaaatgacc caaggaatta ttaggtttaa tcaactgata agagaagata aaagttttga
    24841 aaaaatagta attccgattc gtgatggaat tatgctggcg cggaaaaatt agaactcgct
    24901 actgcaactt ttgtattatt ttcgatgctt cgttcacctt tttctgggcg gctttaatac
    24961 gttgctgata agtgtcgccg caggatgtta gtgatgaaat cctcgtgccc cacgtagcag
    25021 cctgtgaagt taactttgcc gcagaattaa tcaaagcttt atttttcggg ttaagttcga
    25081 atgtacgggc aattttcaaa taccgtgtag caaatttttc atatttgaca actgtctttt
    25141 cgcagttggt gttcttaact actgtctgct cctgttcctt cggaggatta ttctggtcgg
    25201 agctcccacc ctgaagtgtt ttactttgag ccattaccga tatactgcca aataaaatag
    25261 caactgtaac taaaaccgca aaaatgttct tcataatatg tctgtttaaa gtttaatcaa
    25321 agtatttcca gtcttttcaa tgaaaaatgc gaatagccaa ttacaatatt tattcacgat
    25381 ttaactgaac ttataatgtt gcaagataat aaaattttca acgtaaaagc gaactaaaac
    25441 atgttttaca gtttcaaatg acagccccaa ttaaccttcg ggcagcactt gattttttcg
    25501 tggtgcaatt attaaataaa tagctgatag tgtattttga atttctgata attgattatt
    25561 tttaccgagt tgtttttggt ctatgagtaa attattaagt ctgttaaaaa gcaaactgct
    25621 ttggattaac gttggattgg cggttgccat tactattgtt ttggcttgga tagttttgtt
    25681 ttttcttcga atatataccg atcacggaga gtcggttacc gtaccagatt tggttgggtt
    25741 gagtatgaag gaagcggagg aattgtgcga tgataataat tttcagtttg taattaccga
    25801 ttcagttttt tctgacgaag tggataaggg tgctgttgct gaacaaaacc ctgtggccgg
    25861 gtttaaagta aaagaaggca gaactattta tctgattgtc aactctacat ccgacgaaac
    25921 cgttcaaatg ccaactctgg agggtgtttc ccttcgacag gctcatgcct tgctcgaaac
    25981 ttacggactg gttgccggaa atctgaaata tgttcctgat atcgccgaaa acgtggtgat
    26041 aaggcaatta tacaaaggtc gtgaaattga accaggaaca aaaattaaaa aaggatcgcg
    26101 tatcgacctt gtgcttggca ttggcttaag cgatgaaaga gcgacagtac ccaatttaat
    26161 taacatgtca tataatcaag cttacaataa gcttcttgat gaattcctca atgttggggc
    26221 tgtggtttac gataaaagta tcagaacaaa aagagactca gcagaagcac gagtatatcg
    26281 ccaatctcct atgcctgata caataaatac tataaatttg ggttctaatg ttgatatctg
    26341 gttgtcgcgc gacacggctg ccttttagag tgatgcctat aattttttga cctatgaaga
    26401 aatgctttct aatactgaca agttttgcct tttcgttgat tgcgttcaca caggaagttc
    26461 ttatcgacgt agttccttca tcgctggtaa cctatagcag gacatacttt tcgggaatga
    26521 acactaaaga aacgatggac tctgacacat tggaactgcc tttttacgat gattttgcaa
    26581 actctgttgt ttttcccagt tctgatttat ggatcgataa aaatgcattt attaacgacg
    26641 gttatgcaaa agaccccatt tccattggag tagctacact cgatgttctc gattcagtag
    26701 gggctgttta ttcctttttg tcttcaacgg taagttcggc tgcagaatgt ttaacatccc
    26761 ggccaattaa cctcaactac actgctgccg attcggtata tctaagtttt tactatcagt
    26821 gcggaggcta tgggaataaa cctgatgttg gcgattcgct tgttctggaa tttactaccc
    26881 ccgatactac ctggacaagt gtttggcagg ccgacggagg tgttgctatg gattcgtttc
    26941 gtttggtact cattccaata acgcaggagt ggcttctaaa aaaaggattc cagttcaggt
    27001 ttaaaaacca tgcaagcatc agcagtaact acgaaccgtc ctggatttcg aacgccgact
    27061 gctggaacat cgattatatc aggctcgaca cagcacgtaa tcaaaacgac acgataactc
    27121 aggatgttgc gtttataagt tatttctcga gcatgataaa agatttcgaa agtgcgccat
    27181 ggaatcactt tgaaccaatt gcttcattaa ataccattga ttcactggtt tatgtttaca
    27241 aaaacaatgg tcctactacg caaaacatta atcgccaact gctaataaaa gatgtttggg
    27301 gaaatgggac tgggttttct aatctcgacg acagtgaaaa tattttacct tttagcacgc
    27361 tatcatatgc taagaatgta aattacacgt tcaactcggg aacagtcgat tcagcgaggt
    27421 tcgagcttaa aggtttcatt aaaactgata ccacactggc acgtaggcca ttccggtgga
    27481 acgacaccat ttgttactac cagacgtttt acaactacta ttcctacgac gatggttccg
    27541 ctgaaaaagg atacgggatt accggacagg gaactaccgg ctctgcagta gcattgcagt
    27601 ttactcccct gcaaaacgac acactccgcg gtgcgtatat ctttttcaat caggtgctcg
    27661 atgagggaaa tatccattac tttttcttta cggtatggga tgacgaagaa ggcgttccgg
    27721 gcgacacgct cgctaaacag ctgggagtac gacctgtgta ttccgacagt attaacgaat
    27781 ttgtgtatta tgctcttgat accccattgc tgatcacggg aacattctac ataggatgga
    27841 ttaaaactac cgacgacatg cttaatgttg gctttgatgt taaccgggat gcatcgcaac
    27901 acttgtttta caatacacaa ggagattgga tatcatcttc tttaccggga gccattatgg
    27961 tgcgaccagt ttttggagtg aacccagatc cttcatgggt aaaacctcag aataccgata
    28021 attatacatt gtttccgaat ccggcaacca atgctatttc aattcgaaat accaatggtc
    28081 aatctcctga caaatgcgcc atttatgata tttcaggaag gctttgcctg gaatcgtata
    28141 atacagagtt tgtaaacatc cattccttac ccgaaggatt atattttatt cgttttgctg
    28201 atcgtggaaa tatccgcaca ttaaaatttg tagtttcacg ctgaaatgag tgaagattca
    28261 acatccctca gcaatcagga agactataaa aatcaggaag aactttttga gcattaccgt
    28321 tttattgttg acaacggtca atccccggtt cgcatcgaca aatatctggc ttcgaaaatt
    28381 gccaacgttt cgaggaacaa aattcaggct gccagcgatg cagggaatat aagggtaaac
    28441 gatacgccca taaaatcgaa ttataaggtt aaaccagcgg atgtaattac cattctgtta
    28501 agttttccga aaatcgaatt cgagttaata gcggaagata ttccaattga agtggtttat
    28561 gaagataacg atgttctggt tgtaaacaaa cgagcaggca tggttgtaca ccccagtctt
    28621 gggcattatc agggaaccct ggtcaatgct ttggcatttc accttcaggg caatgagtta
    28681 ttttcggaaa acaacatgcg ttccgggctg gttcatcgaa tcgacaaaga cacctcaggg
    28741 ttattagtta taggtaaaaa cgaaagagca ctaaatcacc tgggcaaaca attctttgaa
    28801 cataccgcac gccgcaccta tcacgctttg gtatggggta atactgctga ggaaaaagga
    28861 acgattaccg gccatatcgg ccgtcatctg aaagaccgga agataatgga tgtattcgaa
    28921 gatggtagct atggcaaaca tgcggtaaca cattatcagg tattggatcg atttgggtat
    28981 gttacctaca ttgaatgtaa actcgaaaca ggcagaacac atcagatcag ggcacatttc
    29041 agccatattg ggcaccctct tttcaacgat tccgactatg gggggaatca aatccttaaa
    29101 ggcccaatat tcaataaata caaacaattt gtttccaatt gctttgaaat ctgtccgcgc
    29161 caggccttac atgcaaaaac tctgggattt attcatcccg tcactaaaaa agaaatgatg
    29221 tttgattcgt tgattccaaa agatatgcaa gcatgcattg acaagtggag atcttacagc
    29281 ggtagttttt cggtttaatg aggccctccg ggctcagttc ctccctgccc ataacattta
    29341 ccgtattcgt gaagaagttg tttacgaaac tccctttcag cctgatagaa ttttgcaact
    29401 ttttttattg gaattactga tttaaacttc gcataatact caactgacaa atctgacttc
    29461 gatttttgaa gttgcatcat tttatcgagc ttttgctcta attcggcttc ggtcattgag
    29521 gcagactttt ctttaatatc gcggtttaat tcccgttctg atttaaaaat ttcgtcattc
    29581 ttcttttcgt actcattata caatggccaa aacttctgtg cctcagctac agtaaggtca
    29641 agttgtgttg taagaaaagc aaccttttct gccttaatct tctcctcgtc tttaactttg
    29701 tttgtgtgtt gcgaaaatac atctaccgaa ataaatagca ttgcgacaca aataaaaagt
    29761 gttttttttg tttccatatt tctattcctc taattcagca gttaaatcat aattcaacag
    29821 atactcctca attgcttcag agcttacttc agaaatccct gcctcctgag gagtttcagt
    29881 aattgcttcc ataaaaacca attcatcgct taaaaggctt tcttcaaagt cgttatatgc
    29941 tgtgaaattt tctgtctttg aagtatctgg attcacagat ttcttgtcaa caacaaggtt
    30001 caacccaatt ttaataaata tagccagtac aataaaggat gcggccatat acaaccaggg
    30061 ttttattata tcgatggttc ttacacgtgg cttatgatcc cttttaactt tcgacataat
    30121 gttatcgggt aaatcatcga aatacccctc aggcactttg aaagcagttt tatttatgta
    30181 gttaccgtta attcccattt tgtatgataa tacttttttt agacaattgt tttcatgtta
    30241 ggtttaaggt tttttcaagt ttttttacag catgatgata agatgctttt aacgcacctt
    30301 cggatgtacc caaaatttcg gaaatcttat gatacggcaa atcgtcgtaa tacctcatat
    30361 taaaaaccaa tcgctgtttt tcggggagac tcataattgc tttctgaatg ttaatctgca
    30421 attcatcacc atcgaaatat gggtcttcct ttaaagaatt aatcatatag ttttcatagt
    30481 cgaaatcaga aaaaacatgc tgtcgtttct tttttttcag aaaattcagg gtctcattgg
    30541 tagcaattct ataaagccac gtgaatattt ttgagtcgtg ccggaaattt gagaggttgt
    30601 ggtatgcttt tataaatgtt tcctgtaaca catcgtcagt atcgtgatgc aggagaagca
    30661 tttttcgaat atgactgtac aaacgcggac catattccac aacaattgtt ctgaaagcag
    30721 attccacatc cttgttttca acaaaacaac gtattatttc ttgctctttc tcgggactca
    30781 tttatgtaaa aatattctcc ccattgcaat gtaaaattaa aaaacaaatt gttctgagtt
    30841 ttattgctga tttttcttaa aaatatgtta tatgttacat ttcttattat tgggaggctt
    30901 aggtaaaatt ttaattttgt aaaaaaaatg taatatggat gcaaaatttg taaaacaagc
    30961 catggagctt tggcctgaat tagaatggat tagtgaccca aaacttcgtg aacaggttac
    31021 taatacatgg gcacttgcct tatcgaaatc ggttctttct cccgatgatt tgcgcaagat
    31081 accttttacc ctgcttgcag gtcc
//
LOCUS       DAOOQP010000023        30845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1703_length_30845_cov_7.734524, whole genome shotgun sequence.
ACCESSION   DAOOQP010000023 DAOOQP010000000
VERSION     DAOOQP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30845
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_30845_cov_7.734524"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..335)
                     /locus_tag="PLK75_04155"
     CDS             complement(21..335)
                     /locus_tag="PLK75_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOY38470.1"
                     /translation="MTPKETYEKLIVSTLKSVYDPEIPVNVYDLGLIYLIDIDDDMNV
                     KINMTLTAPNCPMADDLVEMVRYKVSLIEGIGKVSVSIVFDPPWDTNMLSDEAKLELG
                     LL"
     gene            complement(352..777)
                     /locus_tag="PLK75_04160"
     CDS             complement(352..777)
                     /locus_tag="PLK75_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOY38471.1"
                     /translation="MTINQIQDKIIEEFSVFDDWMDKYAFLIDIGNELAEMSPELKTD
                     DNLISGCQSKVWIYAEMKDGKVYYAADSDAIITKGIASLLIKVLSGQPATDIYTSDLY
                     FIGKIGLDQHLSPTRSNGLLSMIKRMKLYAMAFSADSQK"
     gene            complement(888..1205)
                     /gene="trxA"
                     /locus_tag="PLK75_04165"
     CDS             complement(888..1205)
                     /gene="trxA"
                     /locus_tag="PLK75_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOY38472.1"
                     /translation="MALEITDQNHSEIVNQNEKPVVMDFWATWCGPCRMIAPYIEEMA
                     QTYHEKAIIGKVNVDENPEVSAKYGIRNIPTVIFLKNGEIKDKVVGAVPKSTLAAKLD
                     ALL"
     gene            complement(1251..5012)
                     /gene="dnaE"
                     /locus_tag="PLK75_04170"
     CDS             complement(1251..5012)
                     /gene="dnaE"
                     /locus_tag="PLK75_04170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOY38473.1"
                     /translation="MDFVHLHVHSHYSILDGAASIEGLVAKAAEYKMKALALTDHGYM
                     FGIKAFFDECKKKSIKPLLGVEAYLAEKGHKSKAGKEEYSGYHVILIAKNKIGYHNLV
                     KLCTIASTEGFYQRPRIDRELLEKYGEGLIVSSACLGGEIPQKILLNDFDAAEKAIEW
                     YKNLFGSDFYLELMLHFSDDPEKNKSVYAEQLIVNKYLVDAAKRHRIKLIATNDIHFI
                     NKADADAHDLLICLTTGKSIDDPNRMRYTKQEYFKSPDEMYELFGDNLEALTNTCEIA
                     DKVEYFDLNSKPIMPYFKIPESFATLTEYEKKFREHDLILDFGQEKYNKLDGSFTDKL
                     RIKLESDYLEQLTMAGAVQKYGDPLPDEVLERIRFEIDTIKTMGFPGYFLIVQDFVKY
                     AKDHGVIVGPGRGSAAGSIIAYCNGIIDIDPLKHDLLFERFLNPDRISMPDVDIDFDD
                     DGRQSVLNYVTEKYGKENVAQIVTFGTMAAKMVIKDVARVLGLPLSDAERLTKLLPDL
                     GAKLANAYEIILSGESRLGSIEKVIEETNKEITSAKKEGNDSILLVLETRKIIASEII
                     DARAKKDSKRLQTLQFACTLEGSVRQTAVHACGVIIGRDELQNHIPLMPSKESSVLCT
                     QYDGHFVESIGLLKMDFLGLKTLSILREAIQNVKEYKAISIDIHNIPFDDEKVYELFS
                     KGETTGIFQFESDPMKAHLRNLRPNRFDDLVAMNALYRPGPMEYIPSFINRKLGREKI
                     DYDHPMMEKYLEKTYGVTVYQEQVMLLSRALAGFTRGESDQLRKAMGKKNEKLMQELK
                     AKFFEGCHKNPQFTEGCKRANKPAQDLIEKIWKDWEAFAKYAFNKSHSVCYAHLAYQT
                     AYMKAHFPAEFMAASMSRNLSDIKEITKLMEECRRMKMKVLGPDINDSSYKFKVTFEG
                     NLRFGLVAVKNVGTNAVTDIIKEREKNGPFTDIFNFAERVNLNAVNKKNIEALAYSGA
                     FDCFDSIERHQYFLSSENGQTSFIENLISYGNAMQARKNGSMDLFGDIITVDTKKPGI
                     PPATNVYSTEWLNHERDHIGVYISAHPLDAYSFEIKNLNTTPLADLKDLSIHQLDETH
                     HVIGLITKAEEKIGKTGNPYGKLIIEDYSDNFSINFYSKDYLDYKMFFQEGQSVMITG
                     TVKKGFKNPDERIFQTKNIRWLAELKENYFHSLELTLNIADITEDFVGTLTKIVKKNK
                     GKIKLYLIFEESEKTLSIKTHSRTYNIELSDDMIHFVENCSTIRRCKLN"
     gene            complement(5190..7127)
                     /locus_tag="PLK75_04175"
     CDS             complement(5190..7127)
                     /locus_tag="PLK75_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOY38474.1"
                     /translation="MKKISFAFIGLIFSVMLAAQDDSFFSEKNFATRLFSQVHEVDPR
                     LYQYNIGFYHLNLDMDNQSTAVAGHTLMYLNFQETYEGELVFDLMQCMDVDSVLINGV
                     NTAFTHQDNLLIIPYSPATPFSSDYQVCADIYYSGLPDAGMTCNNYEFLSTNYKITYS
                     LTEPYEAKYWFPCKQVLTDKADSVWVFVTIPNDLLAGSQGLLTNTVDLGNGKKRMEWK
                     SNYPIAYYLISVAIGDYQDYSFYVDLSGYDDSVLVQNYVINNAMYLPANEWFIHQTDT
                     MIDVLSVLLGKFPFHEEKYGHCLVNLGGGMEHQTMTTLGNFGFRLVIHELGHSWFGDY
                     LTCATWQDIWINEGFASYVEYFGEEFIQPDGYEDAWLEEAQNRAKLSPTGSVYVPFNE
                     LNDESRIFNYELSYRKGACLVHMIRYMINDDALFFAMLKDYISTFGNSNVTSDDLKNF
                     ISDYTGIDFTTFFENWCYGEGYPQYEIEWYQSPNFLHIRSNQTTVMPDATPFFEIPME
                     YEIVYDSGDRDTVRLEQTQNLQDYSLEVSGLVTEINPDPNNCILKDIISVNHSDIEAY
                     ERPLISVSPNPTANFLEITSSGKIASVLVCSSSGTKVISKHVGDFQLRLDVSMLTPGL
                     YSLVLVHENSQTYIRKFVKNN"
     gene            complement(7721..8395)
                     /locus_tag="PLK75_04180"
     CDS             complement(7721..8395)
                     /locus_tag="PLK75_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOY38475.1"
                     /translation="MEIIHQLTQLYQAHRNPEYAAQMSRYMKNRFPFFGMQSGPREML
                     HQEFIAQHGLPDYETTKEMVLQLFALPEREYHYFAILLLIKHKKNRQPGDIALLERLL
                     TSNSWWDSVDYINGSVLGDFFLKYPDLMQPVTDKWSLDENIWLKRSSIIFQLKYRQNT
                     RLDLLASHILQNNSSKEFFVQKAIGWALREYSKTDAQWVEHFVHNHELKPLSRREAMK
                     RIEKSR"
     gene            8603..9148
                     /locus_tag="PLK75_04185"
     CDS             8603..9148
                     /locus_tag="PLK75_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOY38476.1"
                     /translation="MALKIRLARHGRKGIAYYHIVVADSRSPRDGRFIERIGMYNPNT
                     NPATIELEFDKALSWMQKGALPTDTCKRILSYKGVLMKKHLLEGVVKGAFDSSEAEVR
                     FDKWMNEKSAKVVAKVDRLANEKNKSDKEKLAAETKVKEERAQALARKYAEEAAKAKQ
                     AEEAEQTETPAEEEATAPENE"
     gene            9166..9693
                     /gene="rimM"
                     /locus_tag="PLK75_04190"
     CDS             9166..9693
                     /gene="rimM"
                     /locus_tag="PLK75_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOY38477.1"
                     /translation="MIEETSCQFIGVISKPVGNKGFLQIKIAENADTDLLFNTNSVFL
                     LLDGLFVPFMLEEVEVLSKSAIMKIAGVNTIDKARQYSDIKVYIQESFSDDDTDSDES
                     NQENILGYEVVDVQSGYIGKIRSVNHIPENPLLEVDYKNRTILIPAHDDFIENIDDTL
                     RVIYVKLPEGFLEIF"
     gene            9751..10644
                     /locus_tag="PLK75_04195"
     CDS             9751..10644
                     /locus_tag="PLK75_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38478.1"
                     /translation="MENTVKKDKTTKILIALISVMIVGIGVLIWMVVDLNAKNKNLTT
                     QTITISADKELVKQELNNLLADYEDLSTNNDSLNSELEKEKAHIKTLIREIENMKSVS
                     IAQMEKYKEELNTLRSIMKHYVYQIDSLNTLNQDLMAENENIRMDNERLNYEYEEIVE
                     RNDELEVVVEKASMLKANGINVIYYNSRGKETTKASKIVKLKTVFTLVENSLAKQGNT
                     TIYLRIIRPDGFVMTSAGKTLDIHGESIAYSTSRQVLYEGSNLEVAIYFDVPSTLTSG
                     KYSAELFVENYLIGGTNFLIE"
     gene            10676..11338
                     /locus_tag="PLK75_04200"
     CDS             10676..11338
                     /locus_tag="PLK75_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOY38479.1"
                     /translation="MNIIPIPDNTQFVVIIVITFISLVLLAWVRTSGSKYLNEIFPAV
                     VFERKAYKLWVENSSVSAGIAALLNVILFFNLSVLVWEFIFINRIPVHPSFLYILVPG
                     LILSFFAAKQLLYYLIGSATDQTETTSVYVQGVMIIYRVFGLSLFPVIIAAPFCSSWL
                     SMALFVTAAALFVSAQLLIWYRAFVFCKKTKFSIFYLFLYLCTLEIFPVLFIFKIALS
                     LM"
     gene            11368..12129
                     /locus_tag="PLK75_04205"
     CDS             11368..12129
                     /locus_tag="PLK75_04205"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOY38480.1"
                     /translation="MAVKIKTILVSQPKPENDRSPYSELARKHNLKIDFRPFIQVEGV
                     DAKEFRKTRVEVICHSAVIFTSRLAIDHFFRICEEQKIPISDSWKYFCVSESIANYLQ
                     KYIVYRKRKIFFGNGTFDDLMTDINKNKEEKFLLPLSNPHKQDIPELLDKTGINYTKA
                     VLFQTVSANLSDLSNVNYDVLVFFSPAEIASLFQNFPDFVQNDTKIATFGEATAKAVR
                     DARLRIDIEAPVPECPSMVMALESFICQYNRENKK"
     gene            12163..12603
                     /gene="rnpA"
                     /locus_tag="PLK75_04210"
     CDS             12163..12603
                     /gene="rnpA"
                     /locus_tag="PLK75_04210"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOY38481.1"
                     /translation="MTTQEKNDFRLRKSEIISKDTEIKPLFMKGNYFMQSGLKTVFLV
                     TKTDAAGQVKVLFTAPKKNIRLAVNRNRVKRLMREAFRQNKMEICASATENKKDVTIA
                     FIITSSADATWGEIERKIILSLQRICKTIEINPKFNEIQKGKIQ"
     gene            12575..14260
                     /locus_tag="PLK75_04215"
     CDS             12575..14260
                     /locus_tag="PLK75_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY38482.1"
                     /translation="MKFKKVRFNKFSFLSLFFLLGSLMLLPAEVRAQEDKDFETVKQL
                     DIFYSLYKELDLFYVDEIDPAKTIRIAIDAMLEKMDPYTVYLPEDEIEDLRFMTTGEY
                     GGIGALIRKYEDYVAISEPYQGFPAQKSGLKAGDIIVAIDGKDTKGKNTSDVSELLKG
                     QPGTNVKLSIRRTGENAAMEITITREEIKIPSVPYYELLKGDIAYIQLSSFTNTATTE
                     VRDAYLELSQNRKLNGVILDLRGNPGGLLVEAVKICNLFLEKDVLVVSTKGKVEQWDK
                     EYKTTELPLDATIPLVVLVNSGSASASEIVSGCMQDLDRGVIIGQKTYGKGLVQTTRD
                     LSYNSKLKVTTAKYYIPSGRCIQALDYTHRNEDGSVGRVPDSLVSEFKTRNGRSVFDG
                     GGVLPDIVTEPRKIASITESLILENFIFDFATDYTAKNNTIAGAAEFHIDDALYGDFV
                     SYLSDKHFEYQTESEELLNELIETSKEEKYFSVAEAELEALRLKLGHDTNKDLLVFKD
                     EISEILGMEIVHRYYYQKGKIQYEMKYDEELNKAIEVISNKAMYDSILGVKNK"
     gene            14278..15273
                     /gene="trpS"
                     /locus_tag="PLK75_04220"
     CDS             14278..15273
                     /gene="trpS"
                     /locus_tag="PLK75_04220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOY38483.1"
                     /translation="METVLSGIRSTGNLHLGNYYGAVKNFVRMQHSNHCFFFIADYHS
                     LTTHPNSADFHSNVKQVLIEYLAAGLDPEVSTLYIQSDVPEIFELYLILNMLAYKGEL
                     ERTASFKEKARKQPDNINAGLLTYPVLMAADILIHRADKVPVGKDQEQHLEMTRKYVR
                     RFNTLYQTEFFKEPEPYNFGNALIKIPGLDGTGKMGKSEGNGVFLADDSEVIRKKIMK
                     AVTDTGPTVPHSEPTEPIKNLFAIMEVVSKPETLQFFREQYANCSIRYGDLKKQLAQD
                     VVEAIEPIGKRIKELSRDNDYIARVAKTGAEKARASASKTLAEVRKIIGVTSLQN"
     gene            15295..16806
                     /gene="gltX"
                     /locus_tag="PLK75_04225"
     CDS             15295..16806
                     /gene="gltX"
                     /locus_tag="PLK75_04225"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOY38484.1"
                     /translation="MNRVRVRFAPSPTGPLHIGGVRTALFNYLFARKNNGSFILRIED
                     TDSVRYVEGAEKYIIESLKWCGLQFDEGIEQGGPFEPYRQSDRNSIYRQYADFLLNKG
                     FAYYAFDKPEELDALRHACEKEGKTFVYSPSTRLGLCNSLSLTENEWNDKLLQGAPFV
                     IRFKTPVNTKVTVHDIIRGEVVFQSDELDDKVLFKSDGTPTYHLANVVDDHLMEISHV
                     IRGEEWLPSLPLHVMLYGAFEWTPPEFAHLPLILKPSGKGKLSKRDGDQLGFPVFPHR
                     WVGPDGDISSGYREAGYFSEALLNFLVLLGWNPGDDQEIMSLDEMIQKFDLEKVGKSG
                     SRFDPEKAKWFNHQYLIRKTDEEIATLFMDTLREKGLNPPFATVVRVCSAVKERVSFV
                     QDIWDEAYFYFTPPAEYDTVFVQKQWKPESAGYLMSVAEILKVTANFTASDCESNVKV
                     WIEQNQLSFGKVLNPLRLAIVGAGKGPHIFDIISMIGKDQTLSRIKAAVNTLR"
     gene            complement(17426..19030)
                     /locus_tag="PLK75_04230"
     CDS             complement(17426..19030)
                     /locus_tag="PLK75_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38485.1"
                     /translation="MTRIPKYLILFFASWAMFLPLQAQLQHLECGWEYYALQERLTGN
                     AHSSIYPLYRCDVVDTTLIAPKDLLNYTFTNYEADGKYYRVKKFIFSAQPLIHSEILF
                     NTGSNFSGGYGAGVLFDAEYNNTIATHFDLSYVNFAMTDYINEFRLRTGVLPGTGRVI
                     KQRFPQNIYSNFNITYRPFQLITLEAGFGKHFWGDGYRSMMLSYNAYNYPYIMADLNV
                     WHVQYRFVVAQMKDINVPEGTVWGDFKTKYAYIHYIDWKVCKWFNFGAFEAIISGVNH
                     GFEPGYLNPVIFYRPVEFSLGSVDNALMGINLKFKLLKNTYLYGQFVLDDFNVDEFMN
                     DIRHLRNPDDSEIHYGFFGNKVAGQIGLKSSNIGGINYLHAFTEFNFARPYTYSHVVS
                     QQNYGHFNQSLAHPLGANFVESVSGISYLHKRFLFEGKFMLAFTGLDTTGTHFGQDIY
                     KPTMDALQNGNIPVSTWYNRIGQGIETNIIYTELSAQYFINPDRNLSAIISIGLRHQQ
                     NELATQQNVLIKIGVSGLLIRRYTDF"
     gene            complement(19032..20447)
                     /locus_tag="PLK75_04235"
     CDS             complement(19032..20447)
                     /locus_tag="PLK75_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38486.1"
                     /translation="MPLAIGNKFYCFDQGYHSAIHCSAFSELKINNFADYTTDDGSWI
                     KRKLFHEHLGFVQGENFKIIADPLFEFTYSRDVERNENMYENTRGLMLYAELYKKITL
                     ISSYYENQASMPGYIDDYYNVFRVIPGEARVKPYRNDAYDWGTAYASLSYKPTRFLYV
                     ETGNDKNFIGDGYRSLILSDYAPQYLYFKTVLSAKNFFYMNLATRTLNPNYNNINGDT
                     PDWSENSLYPHKFVNINYLGYNPTLFLQLGVFEATAFGTNAQFSKKITAFFPGLNEAL
                     YHTNDTLHFIWGGNFLFSPVSNVGIYGQFAYGQKQAAAYQAGCKVTIVNTRWMRFIFG
                     RIEWNYVGAGMYQPLGNENYWGHYNQVLAHPAGNDFSEIIVQAAVSRIRFKVSLTGGM
                     IYYRTGLGARGIFSANINDAMEPDRVVFSQARIAYVVNYAYHWQFFASATYYSRKNSG
                     EHYIMPSVGIKTCLRNQYHDF"
     gene            complement(20543..22312)
                     /gene="sppA"
                     /locus_tag="PLK75_04240"
     CDS             complement(20543..22312)
                     /gene="sppA"
                     /locus_tag="PLK75_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOY38487.1"
                     /translation="MKFWKIFLACLLGSFVAMILYVVFMFFSFGVLLSSLSPSAPAIK
                     DNSVLKITLSEDIPDRTNENPFVNFDFATFKTSQVVGLNDILKCLEKAKTDEKIKGIF
                     LDVSSVPAGMATLEEIRNALIEFKETGKFIISYSDAYTQKSYYLASVADKIYLNPQGG
                     LDWRGMYAQVTFFKGTLEKMGIEVQVFRHGKFKSAIEPFLLDKMSEENKLQTLTYVSS
                     IWNHMLTGISVKRNITVADLNLYADSLMIFDAKSALQYKFVDKLVYRDEVLTEIKTKA
                     SYSDKDEDYLVSLGTYSKIPVKAKKEKYSENRIAVIFAEGSIVDGDGKDDEIGGDRLA
                     AEIRKAREDEKVKAVVLRVNSPGGSGLASEIILHEMELTKAVKPVVVSMGNYAASGGY
                     YIACKASYIFAQPNTLTGSIGVFGLLPNMQKLLTDKIGLSFDGVGTNAHSDMGSITRP
                     VTTQEGMVIQKQIEDFYSVFINHVAQGRSMTVAEVDSIGQGRVWSGENALQIGLVDEI
                     GGLDKAVKKAVELAKLTDYRIKELPEVKDFFETMMSDFSNQMEQSYIKKNLGEDYRYI
                     EMLQYVKNASGVQARIPYQIDVY"
     gene            complement(22406..23029)
                     /locus_tag="PLK75_04245"
     CDS             complement(22406..23029)
                     /locus_tag="PLK75_04245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY38488.1"
                     /translation="MKTNYSIPKTALRLMPDVVCNIPGSVPCLYLTFDDGPIPGSTEK
                     ILNVLKQYNVPASFFCTGKQVEVHPEIYRQIITQGHTTGNHSYSHTRLNVLHKKAFIS
                     EIEKTATLVDSVLFRPPYGYITPAMSRIIALHYRIVLWDVMPGDFNSNTDAKTLFRNV
                     KQHASNGSIIVLHDSAPNIENMLDCMKRVIEYYMRQGWQFLKVPSTF"
     gene            complement(23019..26375)
                     /locus_tag="PLK75_04250"
     CDS             complement(23019..26375)
                     /locus_tag="PLK75_04250"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOY38489.1"
                     /translation="MFNSTKTNKIIGWSIWLVAMLVYLISLAPSAGFWDSSEWIAVGY
                     KLQVGHPPGAPFYVLLARLFTLIFAFGSGSYAAFAVNLLSAFASAFTILFLYFTIVML
                     LQRITVKNTPEFSRVQQIMIWGSSAVGALAYAFSDSFWQSATEAEVYSLSSLLTAAVF
                     WCMLKWEKISEKPGAERWLFLITYLMGLSIGTHLLNLLTIPALVFVFGFKKYKPNLKN
                     ILLLAGFAIALLFAILYLLIPGVSNAVAYTDVLFVNVFHLPIGSGMITGFLILYAIFA
                     VSIFIAIRKKLQRLRIILTMVLLLLTGYSSYALTVLRSSAGTPINEADPSQAYNLLSY
                     LEREQYGDRPLLKGPFFNSVLNPQKPYIEASPLHRIVNGKYVITGYQMQPNYIKQDTR
                     YFPRMWSSKENHTEAYKEWSGNNGDKIPSMKENLRFLFRYQIGHMYFRYFMWNFVGRQ
                     NDIQGHGGPVNGNWISGIGFIDRLRLGETSVDSNSILKNPAKNTYFFIPLLVGLIGLV
                     FHFRKDKKASGILLLLFFFTGIAIVIYLNQFPYQPRERDYSYAGSFYTFSVWIGIGCY
                     ALFYLLKKKWQTLRVAIITTSVCLLAVPVLMLAENFADHNKSGQRLASEMAYNYLSGL
                     APNAILFTNGDNDTYPLWYLQEVEGVRTDVRVINLSYLNSDWYINQCRKKQYESDPVP
                     CNIDASAYLAGTREFVYIHDNVFPFVDKIYESHKAEFDHSMEQIYAQWVGMLDKSLYP
                     QMKPDEYEKLKKDFMFMAPHGKNPQFKAFRSFIENISSPEKAKLYKIDLTIANAILLA
                     IDNMLKAQIEKPIPLADALAFVFSDNESNKMQHSYSDKPENYFPGTRLIMPLNKEHLL
                     STGTVKASQKEWIPDAMEWEIPKKLLTKSDLIVLEIINENNWKRPVYFSITAGSESYL
                     GLEKYFSLEGMSFRLAPIINELSQKSIGSVDAEMIYKNLTENFRLYSYTDSSVYLDDN
                     MRQLAGNIRNIYGHCARALYFEGRIKESESMLDACLELIPNNRVAYEYYTTTLIHGYY
                     RINKKKKARETAQILAANSVEYLSFLLSFDEKYQPSLDKEEKKTLLILQQLNRMAKEY
                     KHREYLPEIESQYRNMRTAYEKKKGKSFEN"
     gene            26528..27172
                     /locus_tag="PLK75_04255"
     CDS             26528..27172
                     /locus_tag="PLK75_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38490.1"
                     /translation="MKTLFTIPFIFLALLGTAQNVTEGEAIISGVKQNAIIATIPKAD
                     VEVISKEWKNLMKKYDAEVTGKDELFANNAVIKSITDNSMDVYARFEVNKDKDVVMSV
                     AFDLGGFYMNSSAQPELFKEAKKFVQKFAESQSKSAYKETVKAEQKVLSGMEKEKKKL
                     EKANEKLEKKNEDMRNQIKENSQAIKENQAQIDNKIKEIETQENKVKALKKDKP"
     gene            27271..27963
                     /gene="purQ"
                     /locus_tag="PLK75_04260"
     CDS             27271..27963
                     /gene="purQ"
                     /locus_tag="PLK75_04260"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOY38491.1"
                     /translation="MKFGVVIFPGSNCDHDMKYSLQDILLQDVVELWHKDSSLKNCDA
                     IAIPGGFSFGDYLRSGALARFSPIMTKVIQFANSGGFVFGVCNGFQILCETGLLPGAL
                     LHNTNQKFICRNQFIKAEHNQSAMTNLVPTNKALKIPIAHGEGRYYAPNDVLSDMVNH
                     NQIVWRYCDANAELSEKSNPNGSVMNIAGICNRKGNVFGMMPHPERAADPELGNTDGV
                     LILQSIINHLQK"
     gene            28066..29604
                     /locus_tag="PLK75_04265"
     CDS             28066..29604
                     /locus_tag="PLK75_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOY38492.1"
                     /translation="MLVKIYASAVQGISARLITIEVNASKGYAYHLVGLPDNAVKESQ
                     HRIVSALSNNELRWPGKKIIINMAPADVRKEGSAYDLPLAIGVLAASQQIVAENTDRF
                     VIMGELSLDGSVKPVKGALPIAIDAQKAGFEYLILPVENAREAAVVKGIKVLGVSHIT
                     EVIDIFDKQVFPEPVSVDIEAEFAHAEDMLMPDFSEVKGQENVKRALEIAAAGGHNII
                     LIGPPGAGKTMLAKRMPGILPPLSLDEALETTKIHSVAGKMNGNCSLVTHRPFRSPHH
                     TISDVAMVGGGSYPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRMVTISRAK
                     VTIEYPAALMLIASMNPCPCGFYNHPDKKCLCTPSMVQKYLSRISGPLLDRIDIHLEV
                     VPVPFDRLAEKKNGETSAEIRKRVVEARKIQLARFQGAKGVYCNAQMSSRQLRKYCPL
                     DESSLRLLKTAMERLGLSARAYDRIIKVSRTIADIETSENILTSHIAEAIQYRSLDRE
                     GWAG"
     gene            complement(29636..30700)
                     /locus_tag="PLK75_04270"
     CDS             complement(29636..30700)
                     /locus_tag="PLK75_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOY38493.1"
                     /translation="MDFKDSIKQLAERVVKLKNQIQTEEATKNAFIMPFIQILGYDVF
                     NPIEVVPEYTADLGLKKGEKVDYAILKDNIPQILIECKWWGENLDVYNSQLFRYFHTS
                     KAKFGMLTNGIQYRFYTDLIEPNKMDDKPFLEFDITSMKEQAINELKKFHKSYFDINT
                     IFNSASELKYSNEIRTILTNEFNNPSANFVRYFVKQVYSGQATERIINQFTEIVKRSA
                     NQLISDIINERLKSALDKENAKEIEQAKIDADKKAEEANKVVTTDEEIEAFFIVKSIL
                     REKIDCSRIFYRDFQNFFSILLDDSIRQTICRFYFKEDKKSIGVFDENKKEIKHDINK
                     LDDIYLYSDILHQHLVKIAN"
BASE COUNT         8995 a         6345 c         6436 g         9069 t
ORIGIN      
        1 tggtttctgt gtttattgca ttacaacaat ccaagctcca gcttggcttc atcgctgagc
       61 atgttcgtgt cccagggagg atcgaaaaca atgctaacgc ttactttccc gataccttca
      121 atcaaactga ctttataacg caccatttcc accagatcgt ctgccatcgg gcagttggga
      181 gcggttagcg tcatattgat ttttacgttc atatcatcat cgatatctat cagataaata
      241 aggcctaaat cgtaaacatt aaccggaatt tcgggatcgt aaaccgattt cagggtggag
      301 acaatgagtt tttcgtaagt ttcttttggg gtcatcgcat caggtattag cttatttttg
      361 ggaatcggca ctgaaagcca tcgcgtacaa cttcattctt tttatcattg atagtagtcc
      421 gttgctgcgt gtcggggata gatgctggtc aagtccgatt tttccgatga aatataaatc
      481 actggtataa atgtcggtgg cgggttgccc tgaaagcact ttaattagaa gtgaagcaat
      541 ccctttagta ataattgcat cactgtcggc agcataatat actttcccat ctttcatttc
      601 agcataaatc caaactttcg actggcatcc cgaaatcagg ttatcgtctg tcttcagttc
      661 agggctcatt tcagccagtt catttccgat atcaattaaa aaggcgtact tgtccatcca
      721 gtcgtcgaaa accgagaact cttcaataat tttatcttga atctgattta ttgtcatcgt
      781 ttatcgctaa attttttcta tcaactacac caaataattt accggcattt ctgaatattg
      841 taaccggaat tgcctgaata ttgttgttaa ttttctggtg gtttttttca gaggagtgca
      901 tccagttttg cagcaagggt cgatttggga acagcgccaa ccaccttatc cttaatttcc
      961 ccgtttttta gaaaaataac agtaggaata tttctgattc cgtattttgc cgacacttca
     1021 ggattttcat ctacatttac tttcccgatg atggctttct cgtggtatgt ttgagccatt
     1081 tcttcgatat aaggtgcaat cattctgcag ggtccgcacc atgttgccca aaaatccatt
     1141 acaacgggtt tttcattttg gttcacaatt tcactgtgat tctgatcggt aatttctaaa
     1201 gccatatatt taagttttac gttagtttat ttctgacgaa tataataaaa ttaattcagt
     1261 ttacacctac gtattgtaga acagttttct acaaagtgaa tcatgtcatc cgaaagctcg
     1321 atgttgtaag ttcggctgtg tgtttttatg cttaatgtct tttcgctttc ttcaaaaatt
     1381 aaatagagct ttattttccc cttgttcttt tttacaattt ttgtcagagt tccaacaaaa
     1441 tcctcagtga tgtcggcaat gttcaatgta agctccagag agtgaaaata attttcttta
     1501 agctctgcca accaacgaat attcttcgtt tgaaaaattc tctcatcagg atttttaaat
     1561 ccttttttca ccgttccggt aatcattacc gattgccctt cctgaaaaaa cattttatag
     1621 tcaagataat cctttgaata aaaatttatc gaaaagttat cggaataatc ttcaataata
     1681 agcttcccgt agggatttcc ggtctttcca attttttctt cagctttggt aatcagcccg
     1741 attacatgat gtgtttcgtc aagctggtgg attgacaaat cttttaggtc ggcaaggggt
     1801 gtggtattta gattttttat ttcgaatgaa tacgcgtcaa gcggatgggc cgaaatatac
     1861 accccgatat gatctctttc atggttgagc cactctgtgg aatatacatt ggttgcggga
     1921 ggaatacctg gtttcttagt atcaacggta ataatgtcgc caaatagatc catactgccg
     1981 ttttttcgcg cttgcatagc attcccatac gaaatcagat tttcaatgaa actggtttgt
     2041 ccgttttcgc tgcttaaaaa atactgatgt cgttcaatgg agtcaaagca atcgaaagct
     2101 cccgaatatg caagagcctc aatatttttt ttgtttacag cattgaggtt tactctttcg
     2161 gcaaaattaa aaatatcggt aaatgggccg tttttttcac gctctttaat aatatcggtt
     2221 actgcatttg taccgacatt ttttacagcc accagtccaa atcgcaggtt gccctcgaag
     2281 gtaaccttga atttatagct gctgtcgttg atgtcgggac ccaacacttt cattttcatt
     2341 ctgcgacatt cttccatcaa tttggttatt tccttgatgt cggataagtt tcggctcatc
     2401 gatgcagcca tgaattcggc agggaaatgg gctttcatgt aagcagtttg ataagccaga
     2461 tgcgcatagc atacagaatg cgacttgtta aaagcgtatt ttgcaaatgc ttcccagtct
     2521 ttccatattt tttctatcag gtcttgtgcc ggcttgttgg cacgtttgca accttcggta
     2581 aattgggggt ttttatgaca gccttcgaaa aactttgctt taagttcctg catcaacttt
     2641 tcgtttttct ttcccattgc tttccgcaac tggtcagatt ctccacgtgt aaaccctgcc
     2701 aaagccctcg aaagcaacat aacctgctcc tgatacacag ttaccccata ggttttttca
     2761 aggtattttt ccatcatggg gtgatcgtag tctatttttt cgcgcccaag tttacggttg
     2821 atgaaactag ggatgtactc catgggtcct ggcctgtata atgcattcat cgctacaagg
     2881 tcgtcgaaac ggttgggtcg taaattgcgt aaatgagcct tcatggggtc cgactcaaac
     2941 tggaaaattc ccgttgtctc tcctttgctg aacaattcat aaaccttttc gtcgtcgaat
     3001 gggatattgt gaatatcgat gcttatagcc ttgtactctt ttacattttg tatggcctct
     3061 ctgagaatcg acagggtttt caacccgaga aagtccattt ttaatagccc tatcgattca
     3121 acgaaatgac catcgtattg ggtgcataaa accgaagact ctttcgaggg cattaacggg
     3181 atatggttct gtagttcatc tcttccgatg ataacgccgc aggcatgtac ggcagtctga
     3241 cgcaccgatc cctccagggt gcaggcgaat tgcagggtct gtaatctctt actgtccttc
     3301 ttagcacggg catcaataat ctcggatgca ataattttcc tcgtttcaag cacaagaagt
     3361 atggagtcgt tcccctcttt ctttgcagag gtgatttctt tgtttgtttc ttctattact
     3421 ttttcgatgc tgccaagtcg cgattccccc gacaaaatta tttcgtatgc attggccagt
     3481 tttgccccca gatccggaag gagcttggta agcctttcgg catccgaaag cggcaatccc
     3541 aacacgcgtg ccacatcttt aataaccatt tttgctgcca tggtaccaaa tgtaactatc
     3601 tgggcaacgt tttcttttcc gtatttttcg gttacataat ttagcacact ttgacggccg
     3661 tcgtcatcga agtcgatatc cacatctggc atcgatatac ggtcagggtt caggaatctt
     3721 tcaaacagca aatcgtgctt gagcgggtca atatcaataa taccattgca gtacgcaata
     3781 atcgagccgg cagcagagcc tcttcccgga cccacaatta ctccgtggtc tttggcatat
     3841 tttacaaaat cctgcactat cagaaagtat cccggaaacc ccatggtttt aatagtgtcg
     3901 atttcgaacc gaatcctttc taatacttca tccggcaaag ggtcgccata cttctgcaca
     3961 gctcctgcca tggttaactg ctcaagataa tccgactcaa gctttatgcg aagtttgtcg
     4021 gtgaaactgc catcaagttt attatacttc tcctgcccaa aatcaagaat aaggtcatgc
     4081 tcacgaaact ttttttcata ttctgtgagt gtggcgaacg attcggggat tttgaaatag
     4141 ggcattatgg gcttggagtt caggtcgaag tactcgacct tgtcggcaat ttcacaagta
     4201 ttggtcaggg cttcgagatt gtcgccgaaa agttcataca tttcgtcggg cgatttgaag
     4261 tactcctgct tggtataccg catgcggttc gggtcatcaa tactcttccc tgtggtaagg
     4321 cagatgagca aatcatgggc atcggcatcg gctttgttaa taaaatgtat gtcgttggtg
     4381 gcaatgagtt ttatccgatg tcttttagcc gcatcaacaa gatatttgtt cacaatgagc
     4441 tgttcggcgt ataccgattt gtttttctcc gggtcatcag aaaaatgcag catcaattcc
     4501 agataaaaat cggaaccaaa gagattttta taccattcta ttgctttttc agcagcatca
     4561 aaatcgttta atagtatttt ctgcggaatt tcaccgccaa ggcaggctga agaaacaatc
     4621 aggccttcac catatttttc gagcagctct ctgtcaattc tcgggcgctg gtaaaaccct
     4681 tctgtcgaag cgatggtaca gagctttaca agattatgat agccaatttt gtttttggca
     4741 attaatatta catggtatcc cgaatattct tctttcccag ctttgctttt atgtcctttt
     4801 tcagccagat aggcttctac ccccagcagt ggttttatgg atttcttttt acattcatcg
     4861 aaaaaagctt ttataccgaa catatagccg tgatcggtga gagccagggc tttcatttta
     4921 tattctgctg ctttggcaac cagtccttct atgctggcgg caccatctaa aatcgaataa
     4981 tgcgagtgaa cgtgtaagtg aacaaaatcc ataatgtaac tacttgaata tcagtataaa
     5041 taatttaata tttcctcttc ctaaaagtag taattcaaaa attaaaaaac gcgatgtccc
     5101 tttgattttc aggaaaaagt aatgaacaaa ttgcgattaa taaattatac gcggttacac
     5161 aagctttcgg agttcggctt cagtttttat taattgtttt taacaaattt ccggatatag
     5221 gtttggctgt tttcgtgaac aagaaccaga gaatataaac ccggtgttag catcgataca
     5281 tcgagcctta gctgaaagtc gccgacgtgt ttcgaaataa ccttcgttcc gctggaggag
     5341 cacaccaaaa cggaagcgat tttaccgctc gaagttattt ctaaaaaatt ggctgtcgga
     5401 ttgggcgaaa ccgatatcaa gggacgttcg taagcttcga tatcggaatg atttaccgaa
     5461 ataatatctt ttaaaataca gttgttcgga tcggggttta tttcagttac caatccgctg
     5521 acctcaagac tgtaatcctg taggttctgt gtttgttcca gccgcactgt gtcgcggtcg
     5581 cccgaatcgt acacaatttc gtattccatg ggaatttcga aaaacggtgt cgcgtcaggc
     5641 attaccgttg tttgattact gcggatatgc aggaagtttg gcgattgata ccattcaatt
     5701 tcatattgtg gatagccttc gccgtagcac cagttttcga agaaagttgt gaaatcgatg
     5761 ccggtgtaat cgctgataaa atttttcaga tcgtcggaag ttacattgct gttgccaaaa
     5821 gtgctgatat agtctttaag catggcaaaa aacagggcat catcattaat catatagcgg
     5881 atcatatgca ccaggcaggc tccttttctg tagctcagtt cgtaattgaa tatgcggctt
     5941 tcgtcgttca gctcgttgaa aggcacgtac accgaacccg ttggcgatag ttttgcccgg
     6001 ttctgtgctt cctcgagcca tgcatcttca tagccgtcgg gctggataaa ttcctcgccg
     6061 aaatattcca catagcttgc aaaaccttcg ttaatccata tatcttgcca ggtggcacag
     6121 gtaaggtaat cgccgaacca gctgtgaccc aattcatgaa tgaccagcct gaacccgaaa
     6181 ttgccgagcg tagtcatggt ttggtgctcc attccgccgc caagatttac caaacaatgt
     6241 ccgtacttct cttcatgaaa cggaaatttt ccaagcagca ccgaaaggac gtcaatcatt
     6301 gtgtcggttt gatgaataaa ccattcattt gcaggcaggt acatggcatt attaataaca
     6361 tagttctgca ccagtaccga atcgtcgtag ccgctaaggt ccacatagaa cgaataatcc
     6421 tgataatcgc ctattgcaac cgatatcagg tagtaggcaa tcggataatt cgatttccat
     6481 tccatccgtt ttttcccatt tccaagatca accgtgttgg taagcaaacc ctgtgaccct
     6541 gccagcagat cgttgggtat tgttacgaaa acccataccg aatcggcttt gtcggtgagc
     6601 acctgtttgc aggggaacca gtatttggct tcgtaaggtt cagtgagtga ataggtgatt
     6661 ttatagttgg tcgataagaa ttcgtaattg ttacaggtca ttccggcatc gggtaggcct
     6721 gaataatata tatcggcgca cacctgataa tcgctgctga aaggggttgc cggagaatat
     6781 ggtattatca gaaggttatc ctggtgtgtg aaagctgtat ttacaccgtt tatcaacacc
     6841 gaatcaacat ccatgcactg cataaggtcg aacacaagtt ccccttcgta ggtctcctga
     6901 aagttcagat acatcagtgt gtgtccggca acggctgtgc tctgattatc catatcgagg
     6961 ttcagatgat aaaacccgat gttgtactga tacaaccgcg ggtcaacttc atgaacctga
     7021 ctaaataacc gggtggcaaa atttttctca gaaaaaaaac tatcgtcctg tgctgcaagc
     7081 attacactaa atataagccc aataaaggca aatgatatct ttttcatact atggcaataa
     7141 aattcttcat aaaagtagaa aaattttact ttgattgtca ggtgcatacc gtccaaaata
     7201 aacataagca acttctatta attattggtt gatgcttaac ttgattcagg tttaggctct
     7261 gtttggcaga aatgtaaagt attatcggta gttaaataaa tttcgcctgt aataatatct
     7321 attaatttgc gggaatgcga atgtttttgg gaaggtggtg gtggaatgag tgcgtcgttt
     7381 ctccgcccgt cattgcgagg gttgaaaacc cgaagcaatc tgtacttagg cgggcgatga
     7441 tagattcgcc ttcaggtacg gagattgcta cgctgcgctc gcaatgacgt tggggagccc
     7501 gtcattgcga atgtgaggta cgaacatgaa gcaatctgta tttcgacgag cttcccacag
     7561 gtacttcttt gacggaatta atacagacat ttcaaaaggt tgtgttgtaa cgtcttcgtg
     7621 gatgtggatt gcttcactgc gttcgcaatg acgttgggga gcgcgtcatt gcctttagat
     7681 tagcaccgta aaaattagtt gtaattttat ggtagttcaa ttaccggctc ttctcaattc
     7741 ttttcatagc ttcgcgtcgg cttaatggct tcagttcgtg gttgtgaaca aaatgctcaa
     7801 cccattgtgc atcggttttc gaatattcgc gcaacgccca gccgatggct ttttgcacaa
     7861 aaaattcttt cgagctgttg ttttgcaaaa tatgcgaagc aagcaaatcg agccgggtgt
     7921 tctgtcggta tttcaactga aaaataatag aactccgttt taaccagatg ttttcgtcaa
     7981 ggctccactt gtcggttact ggttgcatca gatcgggata tttcagaaaa aaatcgccga
     8041 gaaccgagcc attgatataa tcgaccgaat cccaccacga attggaggtc agcaatcttt
     8101 cgagcaacgc aatatcgccg ggctgtcggt ttttcttgtg ctttatcagt aataaaatgg
     8161 caaaataatg atactccctt tcgggcaatg caaacaactg caataccatt tcttttgttg
     8221 tttcataatc gggtagccca tgttgtgcaa taaactcctg atgaagcatt tctctgggcc
     8281 cagactgcat accaaaaaac gggaaccggt ttttcatgta acgactcatc tgtgcagcat
     8341 attccggatt acgatgtgcc tgatataatt gggtcagttg atgtatgatt tccatagtgt
     8401 gttttttcca aaattataaa attaactgca aataatggaa actcattgtt caataccttg
     8461 gctgtgggta ttgaatccgg gtaataatca tcggttttcg aaacttttgc ggcaacatga
     8521 tgatattaca aaatattatt tatatttgca gcccggttcg ggaaaccgga gaatgattgt
     8581 ttaatttaat tgataaaaac aaatggcttt aaaaatcaga ctggcacgac atggtagaaa
     8641 aggaattgct tattatcaca ttgttgtagc cgacagcaga tcgccacgtg atggcagatt
     8701 tattgaacgt atcggaatgt ataacccgaa cacaaaccct gcaaccatcg aactcgaatt
     8761 cgacaaggcg ctttcgtgga tgcagaaggg tgcgcttccg accgatacat gtaaaaggat
     8821 cttatcgtat aaaggagtcc tgatgaaaaa acacctgctc gaaggagtgg tgaaaggtgc
     8881 ttttgatagc agcgaggcag aagtgcgttt cgacaaatgg atgaatgaaa agtctgccaa
     8941 agttgttgct aaagttgacc ggttggctaa cgagaaaaac aagagcgaca aagaaaaact
     9001 cgcagccgaa accaaagtaa aagaagaacg cgctcaggct ctggcacgca aatatgccga
     9061 agaagctgcc aaggcaaaac aagccgaaga agctgaacaa accgaaaccc ctgccgaaga
     9121 agaggctact gctcccgaaa acgagtaatc gctgatactg acgcaatgat tgaggaaacc
     9181 tcgtgccaat ttatcggagt aatatcaaaa cccgttggca ataaaggctt tcttcagata
     9241 aaaatagcag agaatgccga caccgatttg ttgttcaaca ccaattcggt gtttttattg
     9301 ttggatgggc tttttgtccc tttcatgctc gaggaagttg aagtactttc gaaatcagct
     9361 ataatgaaaa ttgcaggggt taatactatt gacaaagccc ggcaatatag cgatataaaa
     9421 gtttatattc aggaaagctt ctcagatgat gacaccgatt ccgacgaatc aaatcaggag
     9481 aatattcttg gatatgaagt tgttgacgta cagtcaggat atattgggaa aataaggagc
     9541 gtaaatcata tccccgaaaa cccattgtta gaggttgatt acaaaaacag aacgatactc
     9601 attcctgcgc acgacgactt cattgaaaat atagacgaca cccttcgcgt tatttatgtg
     9661 aaactgcctg aaggatttct tgaaatattt taacatcagg gttcggttaa aattgctaat
     9721 tttactgcct gaaactaaat gaataataca atggaaaaca cagttaaaaa agataaaact
     9781 accaaaatac tgatagctct gatatcggtt atgattgttg gcatcggtgt tttaatctgg
     9841 atggttgttg atttgaatgc aaaaaacaaa aatcttacca cacaaactat tacaatcagt
     9901 gccgataaag agctcgttaa gcaggaattg aataatctgc tggccgatta cgaagacctg
     9961 agcacaaaca acgacagcct aaacagtgaa cttgaaaaag aaaaggccca tattaaaacg
    10021 ctaatccggg aaattgaaaa tatgaagtcg gtgagcattg cgcagatgga aaaatacaag
    10081 gaggaactca ataccttgcg ttctattatg aaacactatg tgtaccagat cgattcgtta
    10141 aatacgctga atcaagatct tatggcggaa aatgagaata tccgcatgga caacgaacgc
    10201 ctgaattacg aatacgaaga aatagttgag cgaaacgacg aactggaagt ggtggtcgaa
    10261 aaggcttcaa tgcttaaagc aaacggaata aacgtaattt actataacag cagaggaaag
    10321 gaaacaacca aagcttcaaa aatcgtaaaa ctaaagacgg tgtttacact tgtcgaaaat
    10381 tcactggcaa aacagggaaa taccactata tatctgcgta taatccgccc cgatgggttt
    10441 gttatgacaa gtgccggtaa aactcttgac atacacggcg agtcgattgc ctactccact
    10501 tcgcggcagg ttctatacga aggctcaaat cttgaagttg cgatatattt tgacgttcct
    10561 tcaaccctta caagtggtaa atatagtgcc gaactatttg ttgagaacta tttgattggc
    10621 gggacaaact tcctcattga ataaaactat atccggatga tgtaatgcat tcgagatgaa
    10681 cataatacca atacccgaca acacccaatt cgtagttatt atcgtaatta ctttcattag
    10741 cttggtttta ttggcttggg tgcgcaccag tgggagtaaa tatctgaacg aaatttttcc
    10801 ggcggttgtt ttcgaaagaa aagcatataa actttgggtt gaaaacagca gtgtctctgc
    10861 cggaattgca gcccttctaa atgtgattct attttttaac ctgtcggtgt tggtgtggga
    10921 atttattttt ataaacagaa ttccagttca cccatctttt ctttacatcc tagttccggg
    10981 attgatttta agcttttttg ctgcgaaaca actattgtat tatcttatag gttctgctac
    11041 tgaccagact gaaactactt cggtctatgt gcagggagtt atgattattt acagggtatt
    11101 cgggctttcg ctttttcccg tgattattgc agcacctttt tgcagctcat ggctttcgat
    11161 ggcattgttt gttaccgcag cagcactttt tgtttcggca caattactca tttggtaccg
    11221 tgcttttgtg ttttgtaaga aaacaaaatt ttcaatattc tacttgtttt tgtatctttg
    11281 tacccttgaa atatttcctg tgttgttcat attcaaaatt gctttgagtt tgatgtaaca
    11341 gagtaaatta attgtaaaca agagaagatg gctgtaaaaa ttaaaacaat attggtttcg
    11401 caacctaaac ccgaaaacga cagatcacct tactctgagc tggcaagaaa acacaacctg
    11461 aaaattgatt tcagaccgtt tattcaggtt gagggtgtcg acgctaaaga attccgaaaa
    11521 acccgcgtcg aagttatatg ccactcagct gttattttta ccagtaggtt ggccattgat
    11581 cattttttta gaatttgtga agaacaaaaa atccctattt ccgactcatg gaaatatttt
    11641 tgcgtctctg agtcgattgc aaattacctt cagaaatata ttgtgtaccg aaaacggaaa
    11701 atattttttg gtaacggaac attcgatgat ttgatgacgg atatcaataa aaacaaagaa
    11761 gaaaaattcc ttctgccgct cagtaacccg cataaacagg acatccccga gctgctcgat
    11821 aaaaccggca ttaactacac aaaagcagtt ttatttcaaa cagtaagtgc aaatttgtcg
    11881 gatttaagta atgtaaacta cgatgttctt gtgtttttca gtcctgctga aatagcttca
    11941 ctgtttcaga attttcctga ttttgtgcaa aacgatacta aaatagccac cttcggtgag
    12001 gccaccgcaa aagctgtaag agatgccaga ctgcgaattg atattgaagc acccgtcccc
    12061 gaatgtccgt ccatggtgat ggcactcgaa tcgtttatat gccagtataa cagagagaat
    12121 aaaaagtagc tcctgccgtc aaccccaaac aatattacag caatgacaac gcaggagaaa
    12181 aacgattttc ggttgcgtaa gagtgaaata atttcgaagg ataccgaaat taaaccctta
    12241 ttcatgaagg gcaattactt tatgcaatcc ggattgaaaa cagtttttct ggttaccaaa
    12301 accgatgcag caggtcaggt taaggtattg ttcactgcac caaaaaaaaa cataaggctt
    12361 gcagtgaatc gtaatcgggt aaaacgactt atgcgcgaag cattccggca aaataaaatg
    12421 gaaatatgtg cttctgccac cgaaaacaag aaagacgtta ccattgcatt cattattacc
    12481 tcatcggctg atgcgacctg gggcgaaatt gaaagaaaaa taattctatc tttacaaaga
    12541 atatgtaaaa ccatagaaat taacccaaaa ttcaatgaaa ttcaaaaagg taagattcaa
    12601 taaatttagt tttttatccc tgtttttttt gctgggatct ctcatgctat tgcccgctga
    12661 agtgcgagca caggaggata aggactttga aactgtaaag caactcgata tattttattc
    12721 tctgtacaaa gagctcgact tgttttatgt tgatgaaatc gacccggcga aaaccataag
    12781 aattgctata gatgcaatgc ttgaaaaaat ggatccctac acagtgtatt tgccggaaga
    12841 cgaaattgaa gatttacgat tcatgactac cggagagtat gggggcattg gtgctctaat
    12901 cagaaaatac gaagattatg ttgcaatatc ggaaccgtac cagggctttc cagcgcagaa
    12961 atcaggactg aaagccggcg atatcattgt tgcaattgat ggcaaagata ccaaaggtaa
    13021 aaacacctcc gatgtgtcgg agttattgaa aggccagccg ggaacaaatg taaaactcag
    13081 tatcagacgt accggggaga atgctgcaat ggaaattacg attaccagag aagaaataaa
    13141 aatcccgagt gttccttact acgaattact caaaggcgat attgcttata ttcaactgag
    13201 cagttttacc aatacggcta ctaccgaagt gcgtgacgcc tatctcgaac ttagccagaa
    13261 ccgtaaactc aatggggtaa tactggacct caggggaaac ccgggcgggt tattggtaga
    13321 agctgttaaa atttgtaatc tgtttctcga gaaagacgtt cttgtggtta gtactaaagg
    13381 taaagttgag caatgggata aggaatacaa aactaccgaa ttgcccctcg atgctactat
    13441 cccacttgtt gttcttgtaa atagcggctc ggcatctgcc tccgaaatcg tttcgggctg
    13501 tatgcaggat ctcgacaggg gagtgattat cgggcagaaa acttatggca aggggcttgt
    13561 acagaccaca cgcgatctga gttataactc aaagctaaaa gttaccacag ccaaatatta
    13621 catccccagc ggaagatgta tacaagcact cgattatacc catcgcaacg aagacggaag
    13681 tgttggaagg gtccccgatt cgctggtgtc ggaatttaag acccgcaatg gccgcagcgt
    13741 tttcgacggt ggaggggtat tgcccgatat tgttactgaa ccgcgaaaaa ttgccagtat
    13801 tactgaaagc ctgattcttg aaaacttcat tttcgacttt gcaacagact atactgctaa
    13861 aaataatacc attgccggtg ctgccgagtt ccacattgat gatgcattat acggcgattt
    13921 tgtaagctat ttgtccgata agcatttcga ataccagacc gagagcgagg aattgcttaa
    13981 cgaactgata gaaacttcga aagaagagaa atacttttca gtggcagaag cggaactcga
    14041 ggccttacgc ctcaaattag gtcatgatac caacaaagac ctgttggttt ttaaagacga
    14101 gattagcgaa attctgggaa tggaaatcgt acaccggtat tattatcaaa aaggaaaaat
    14161 tcaatatgaa atgaaatacg acgaggaact gaacaaagca attgaagtca tctccaacaa
    14221 agctatgtac gattctattc tcggcgttaa aaataaatga gaaaaaagta accaacaatg
    14281 gaaactgttt taagtggaat acgatcgacg ggcaacctgc atctcggtaa ttattatggt
    14341 gcagtgaaaa attttgtaag aatgcagcat tcgaaccatt gctttttctt tattgccgat
    14401 tatcactccc tcacaacgca ccccaattca gctgattttc acagcaatgt taagcaggtg
    14461 cttattgagt atctggctgc cggtctggat cctgaagtat caacactata tattcagagc
    14521 gacgtacccg aaatttttga actttatctt atactgaata tgctcgccta taagggagaa
    14581 ttggaacgga cagcatcttt taaagaaaaa gcacgcaagc aacccgacaa catcaatgcc
    14641 ggcttgctta cctacccggt gctgatggct gccgatatcc ttatacaccg cgctgataaa
    14701 gttcccgttg gtaaagacca ggagcagcat ctcgaaatga cgcgtaaata tgtaagacga
    14761 tttaatacgc tgtatcaaac agagtttttt aaggaaccag aaccctataa ctttgggaat
    14821 gccctgatta aaataccggg tcttgatgga acaggaaaaa tgggaaaatc ggaaggtaat
    14881 ggtgtttttc tggctgatga ttcggaagtg atacgaaaaa aaataatgaa ggcagttacc
    14941 gatacagggc ccacggtacc ccattcggaa ccaacagagc ctataaaaaa cttgtttgcc
    15001 attatggaag ttgtttcaaa accagagact ctgcagtttt tcagagaaca atatgccaat
    15061 tgctccatcc gttacggaga tctgaaaaaa caactcgccc aggatgtggt tgaggcaatt
    15121 gagcctatcg gcaaacgaat aaaagaactt tcgcgcgata acgactacat tgcacgcgtt
    15181 gcaaaaacag gagcggaaaa agcccgcgcc agcgcttcga aaacactggc cgaagtccgg
    15241 aaaattattg gcgttacatc ccttcagaac tgaaattaat taatattatc caaaatgaat
    15301 cgagtacgtg tgcgctttgc acccagtccc acggggccct tgcacatcgg tggagtaaga
    15361 acagcattgt tcaattattt atttgcccgg aaaaacaacg ggagctttat tcttcggatt
    15421 gaagacaccg attcggtacg ctatgttgag ggtgccgaaa aatacattat tgaatcactg
    15481 aaatggtgcg gcttacaatt tgacgaggga attgaacagg gcggcccttt tgaaccatac
    15541 cgtcagagtg accggaattc aatatatcgc cagtatgcag attttctttt aaataaaggt
    15601 tttgcatatt atgcttttga caaaccggaa gagcttgacg cgttgcgtca tgcctgcgaa
    15661 aaagaaggga agacgtttgt ttacagcccc tctacacgcc ttggcttgtg caattcactc
    15721 agtttaaccg aaaatgaatg gaacgacaag ctccttcagg gagccccttt tgtaatccgg
    15781 ttcaaaacac ctgtgaatac aaaagttacc gttcatgata tcatcagagg agaagtcgtt
    15841 tttcagtcgg acgaactgga tgataaagta ctgttcaaat cggatggtac gcctacctat
    15901 catctggcaa atgttgtcga cgatcatctg atggagattt cgcatgtgat caggggggag
    15961 gagtggcttc cttcacttcc gcttcatgtt atgctgtacg gtgcttttga atggactcca
    16021 ccggaatttg cccatctgcc acttatattg aaaccttcgg ggaagggcaa gctctcgaaa
    16081 cgcgatggcg atcaactcgg ttttccggtg tttccacacc ggtgggtcgg tcctgatggc
    16141 gatatatcga gtggttaccg cgaagccggt tatttctcgg aagcactgct caattttcta
    16201 gttttgctgg gctggaatcc gggtgatgat caggaaatta tgagtctcga cgaaatgata
    16261 caaaaattcg acctcgaaaa ggtaggtaaa agcggcagcc gattcgaccc tgaaaaagca
    16321 aaatggttca accaccagta cctgatacga aaaacagacg aagagattgc caccttgttt
    16381 atggatacac ttcgtgaaaa agggttaaac ccgcctttcg cgaccgtagt gcgtgtttgt
    16441 agtgctgtga aggaacgggt gagctttgtg caggatatct gggacgaagc gtatttctat
    16501 tttacccctc cggcagaata tgacaccgtt tttgtgcaaa aacaatggaa acctgagtcg
    16561 gccggatatc tcatgagcgt tgccgaaatt cttaaggtaa cagcaaattt tacagccagc
    16621 gattgtgaaa gcaatgttaa ggtttggatt gaacaaaacc agctttcatt cgggaaagtg
    16681 ctcaatccgc ttcgtctggc tattgtgggg gccggtaaag gtccccatat tttcgacatt
    16741 atcagcatga tagggaaaga tcaaacacta agcagaatta aggctgcggt gaatactctc
    16801 agataaaatc aatttgaata cggggaaatc taaaaatcag tatagcgtct gataagcaat
    16861 cccgataccc caattttaat gaaaacattc tgctgcgttg cgagttcgtt ttgttggtga
    16921 cgcagtccaa tgcttatcct gacttgtccc tttatataga tagctaagcg cacccagttc
    16981 tcaaatacca cataagttta caacccactg taggcaacgc aacgtgggca gtcccgacaa
    17041 aaatgcgagc caacccgagc gtgaataccc gtttaataga aaaacaacaa agggcaagag
    17101 aagcaagcac cttaataatc cgactcaggt ttgcttcacg cagctttgtt ttgtcggcca
    17161 aaggtgaagg gcttgtgttg acaaaacatt actttgttag tttttcatta aacgggtaga
    17221 gcggggagac agcttatttt tgtcttgatt ttttgttact ttttcattaa ggaaaaagta
    17281 agataaagaa tgatttaacg atctttagca tcccaatcgt taatagtgat cactgggttt
    17341 aactcaaagt atccttattt aaatgaacaa ttcaggtggt tgctgtgaat actctcagat
    17401 aaattcagtt ggaatacagt aaaatttaga aatcggtata acgcctgatt agcaatcccg
    17461 ataccccaat tttaattaaa acattctgct gcgttgcgag ttcgttttgt tggtgacgca
    17521 gtccaatgct tataattgcc gaaagatttc gatcgggatt aataaaatat tgtgccgata
    17581 attcggtgta aataatattt gtttcaatgc cttgcccaat ccggttatac catgtactta
    17641 ccggaatatt cccattttgc agggcatcca tagtaggttt gtaaatgtct tgcccaaagt
    17701 gtgttcctgt tgtgtcgagt ccggtaaaag caagcatgaa ctttccctcg aaaaggaagc
    17761 gtttgtgcag gtagctgatt cccgaaaccg attcaacgaa attggcaccc agtggatgcg
    17821 caagactttg attaaagtgc ccgtagtttt gttgcgatac tacgtgagaa taggtgtaag
    17881 ggcgggcaaa attaaactcc gtgaacgcat gtaagtaatt tatgcctcca atgttacttg
    17941 attttaagcc tatctgaccc gctaccttat tcccgaaaaa accatagtgt atttccgaat
    18001 cgtcggggtt cctcaaatga cgaatatcgt tcatgaattc gtcaacatta aaatcgtcaa
    18061 gcacaaattg cccgtacagg taggtgtttt ttagtaattt gaatttcagg tttatgccca
    18121 taagtgcatt atcgacgcta cccaacgaaa actcaacagg ccgataaaaa attaccggat
    18181 ttaaatagcc tggttcaaag ccgtggttta cgccggaaat aatagcctcg aaagcgccga
    18241 aattgaacca tttgcaaacc ttccagtcaa tatagtggat gtaagcatat ttggttttaa
    18301 aatcacccca aacagtgccc tcgggaacat tgatgtcctt catctgggca acaacaaagc
    18361 ggtattgaac gtgccatacg ttcaggtcag ccattatgta cggataatta taagcattgt
    18421 acgaaagcat catcgaacgg tagccatctc cccagaaatg ctttccaaaa ccggcttcaa
    18481 gggtaattag ctgaaaaggc cggtaagtaa tattgaaatt tgagtatatg ttttgcggaa
    18541 acctctgctt tattaccctt ccagtacccg gcaatacacc tgttcgtaaa cggaattcgt
    18601 ttatatagtc ggtcattgca aaattcacat aacttaaatc gaaatgagta gcaatggtat
    18661 tattatattc agcatcgaac agaacaccgg ctccgtaccc tcctgagaaa ttactgccgg
    18721 tattgaatag aatttcggaa tggatgaggg gttgagcaga aaaaataaac ttttttaccc
    18781 ggtaatattt cccgtctgct tcgtaatttg taaatgtata attcaatagg tctttcggag
    18841 caatcaacgt agtatccaca acatcgcagc gatatagcgg gtatatcgaa gaatgcgcat
    18901 tcccggtcaa acgttcttgc aacgcatagt attcccatcc gcattccaga tgctgaagtt
    18961 gcgcctgcaa tggtagaaac attgcccaag aggcaaaaaa caaaatgaga tatttcggta
    19021 tccttgtcat gctaaaaatc gtggtattga ttccgaagac aggtttttat tcccaccgat
    19081 ggcataatat aatgttctcc cgaatttttc cggctataat aggttgctga tgcaaaaaac
    19141 tgccagtgat acgcgtaatt gactacatag gctatccttg cctgagaaaa tacaacccga
    19201 tcaggttcca tggcatcgtt gatattggct gagaaaattc cccttgcacc caacccggtt
    19261 cgataataaa tcattcctcc ggtgagagat accttaaatc ggattctact cactgcagcc
    19321 tgcacgatga tttcagagaa atcgttgcct gccggatgtg ccagtacctg attgtaatgc
    19381 ccccagtagt tttcgttacc gaggggctga tacatacctg ctccaacata attccattca
    19441 atccgcccga aaataaatct catccatctc gtattgacga tagtaacttt gcatcccgcc
    19501 tgataggcag ccgcttgttt ctggccgtag gcaaactgac cataaatacc aacattcgaa
    19561 acaggcgaga acagaaaatt tccgccccaa ataaaatgca gtgtatcgtt ggtgtggtac
    19621 aacgcttcgt tcaatccggg gaaaaatgca gtaatctttt ttgagaactg ggcattggta
    19681 ccaaaggccg ttgcttcgaa aacccccagt tgaaggaata atgtggggtt ataacccaga
    19741 taattgatat taacaaattt gtgaggatac aggctgttct cagaccaatc gggggtatct
    19801 ccgtttatgt tattgtaatt cgggttaagt gttctggtgg caagattcat gtaaaagaaa
    19861 ttctttgcgc ttaaaacagt tttaaagtac agatattgag gcgcataatc cgaaagtata
    19921 agagaacggt acccatctcc aataaaattt ttatcgttgc ctgtttcaac atataaaaaa
    19981 cgggtcggct tatagctcaa gcttgcatag gctgtccccc agtcgtaggc atcgttccta
    20041 tatggcttta cccgtgcttc tcccggaatt acccgaaata cattataata gtcgtcgatg
    20101 taccccggca tcgatgcctg attttcgtaa tagctgctga tgagggtgat ttttttgtat
    20161 aactcagcgt aaagcatcag tccgcgagta ttttcataca tattttcgtt tctttcaaca
    20221 tcgcgggagt aggtaaactc aaacaatgga tcggcaatga ttttgaaatt ttctccctgc
    20281 acaaatccga ggtgttcgtg aaaaagtttt cgcttaatcc acgatccatc atcggtagta
    20341 taatcagcga aattgtttat ttttaattcg ctgaacgcac tgcagtggat agcggaatga
    20401 tatccctgat cgaagcagta aaatttattg ccaattgcaa ggggcatatg attaaaatgc
    20461 agataatcct gagctttcgc ctggcaaaaa ctcaggatta cgataactgc acttgtaaga
    20521 aatgttttca gtgtttccgc ttttaataca cgtcaatttg gtaaggaatg cgagcctgta
    20581 ctccggatgc atttttcacg tactgcaaca tttcgatata ccggtaatct tctccaagat
    20641 ttttcttgat ataactttgt tccatctgat tcgaaaagtc cgacatcata gtttcgaaga
    20701 agtctttcac ttcgggcagt tctttaattc tgtagtcggt aagcttggca agctcaacgg
    20761 cttttttcac tgctttgtcg agtccgccga tttcatcaac aagtccaatc tgcaatgcat
    20821 tttcgccact ccatacacgg ccctgaccaa tagagtcaac ttctgcaaca gtcatgcttc
    20881 ttccctgagc tacatggtta atgaatacgc tgtagaaatc ttcaatctgt ttttgaatta
    20941 ccattccttc ctgggtggta acagggcggg ttattgaacc catatcgctg tgagcattgg
    21001 ttccaacgcc atcgaaactc agtccgattt tgtctgtcag aagcttttgc atattgggca
    21061 gcaatccgaa aacaccaatt gatccggtaa gggtgttggg ttgagcaaaa atgtaactgg
    21121 ctttacatgc aatgtaatat cctcccgatg cggcataatt ccccatggac acaaccacgg
    21181 gcttaacagc cttggtaagt tccatttcat gcagaataat ttcggaagcc aggcctgagc
    21241 ctccggggct attcacccgt agtacaacag ctttaacctt ttcgtcttcg cgggctttgc
    21301 gtatctctgc cgccagtctg tccccgccga tttcatcgtc cttaccgtca ccatcaacaa
    21361 tgcttccttc agcaaaaata acggcaatgc ggttctcaga atatttctcc ttctttgctt
    21421 taactgggat ctttgaatac gtccccagcg aaaccagata gtcttcgtct ttatcgctgt
    21481 atgatgcttt tgtctttatt tcggtaagta cttcgtcgcg atacaccagc ttatcgacaa
    21541 atttatactg cagtgccgat ttggcatcaa aaatcataag gctatcggca tagagattca
    21601 gatcggctac ggtaatattg cgttttaccg aaatgcctgt aagcatgtga ttccagatac
    21661 tcgatacata agtaagggtc tgcagtttat tctcttcgct cattttatca agcaggaatg
    21721 gttcaattgc acttttaaat ttaccgtgac ggaaaacctg tacttcgatt cccatttttt
    21781 ctagggtgcc tttaaagaaa gttacctgag catacattcc tctccagtct aatcctccct
    21841 gagggttgag gtatatttta tcagcaaccg aagccagata atacgatttc tgtgtatagg
    21901 catcggaata actgataata aactttccgg tttctttaaa ttcgatgagg gcgttcctga
    21961 tttcttcgag ggtggccatt cctgccggta ccgacgaaac atcaaggaaa atccctttaa
    22021 ttttttcatc tgtttttgct ttttccagac atttcaatat gtcgtttaaa cccactacct
    22081 gtgaagtttt aaaagtggca aaatcgaaat ttacaaacgg attttcgttg gttctgtcgg
    22141 gaatatcttc cgacaaggta attttcagca ccgaattatc tttaatagct ggagccgaag
    22201 gtgataatga tgagagcaac actccaaagc tgaaaaacat gaagactaca tacaatatca
    22261 ttgcaacgaa gcttccaagt aaacaggcta aaaatatttt ccagaatttc atagcattag
    22321 gttttaagtg agttgttttt tcaaaaataa tcaaaataat actaatgtgg gttggagaga
    22381 gcttttttaa taaaaaatta aggtttcaaa atgttgaggg cactttaagg aattgccatc
    22441 cctgtcgcat atagtattca atgacccttt tcatacagtc aagcatattc tcaatatttg
    22501 gggcactgtc gtgcaaaaca atgatgctgc cgttggatgc atgttgtttc acatttcgga
    22561 acaatgtttt tgcatcggtg ttgctgttaa aatcaccggg cattacatcc cacaatacaa
    22621 ttctataatg cagggctatt attcggctca ttgccggagt aatatacccg tatggaggtc
    22681 ggaacaatac tgaatcaact aaagtggccg ttttttcgat ttccgaaatg aaagcctttt
    22741 tgtgcaacac attaaggcgc gtatgactat atgaatgatt tccggttgta tgcccctgtg
    22801 ttattatctg cctgtaaatt tcagggtgga cctctacctg ctttccggtg cagaaaaagc
    22861 ttgcaggcac attgtattgt ttcagcacat ttagtatttt ttctgttgac ccgggtatcg
    22921 gaccatcgtc gaaagtcagg tacaaacatg gaacgctgcc gggtatgttg cagacaacat
    22981 ctggcatgag ccgtaacgct gttttcggaa tactgtaatt agttttcaaa ggattttcct
    23041 tttttcttct cgtacgcagt acgcatattt ctatactgtg attcaatctc cggcagatat
    23101 tccctgtgtt tgtattcttt cgccattcgg ttaagttgct gtaatattag tagagttttc
    23161 ttttcttctt tgtcgaggct tggctgatac ttttcatcaa aactaagcaa gaaactaagg
    23221 tattcaacag aattggctgc cagaatttgt gcagtttctc ttgctttttt ctttttgtta
    23281 atacggtagt agccatgtat cagggttgta gtataatatt catatgcgac acgattgttt
    23341 ggaatcagct caaggcatgc atccagcatg gattcagatt cttttatcct gccctcaaaa
    23401 tacaaggctc ttgcacaatg tccgtaaata ttccggatat ttcccgccag ttgccgcatg
    23461 ttatcgtcga gatatacgct gctgtcggta tatgaataca gtctgaaatt ttcggtcagg
    23521 tttttatata tcatttcagc atcgaccgac ccaatcgatt tctggctgag ctcattgata
    23581 ataggtgcca gccggaaact cattccctca agcgaaaaat atttttccag ccccagatag
    23641 ctttcactac cggcagtaat tgagaaatac accggacgtt tccagttgtt ttcgttaata
    23701 atttccagta ctattaaatc actttttgtc agcagttttt taggaatttc ccattccatc
    23761 gcatccggta tccattcttt ctggcttgct ttcacggtgc cggtgcttag gagatgctcc
    23821 ttgttaagag gcattattaa tcgagttccg gggaaatagt tttcaggttt atccgaatag
    23881 ctgtgctgca ttttattact ctcgttgtcg gaaaacacaa aggcaagggc atcggcaagc
    23941 ggaatcggtt tttcaatctg ggcttttagc atattgtcga tagcaagcaa tatcgcattg
    24001 gctatagtta aatcaatttt gtagagcttt gctttttcgg gtgatgagat attttcgata
    24061 aatgaacgga atgctttgaa ttgcgggttt ttaccatggg gtgccatgaa catgaagtct
    24121 tttttcagtt tttcatattc gtcgggtttc atttgcggat aaagtgattt gtcgagcatt
    24181 ccgacccact gagcatatat ttgctccatg ctatggtcaa attctgcttt atgcgactcg
    24241 taaatcttat cgacaaacgg aaaaacatta tcgtgaatat acacaaattc acgtgtccct
    24301 gcaagataag cggatgcatc aatgttgcac ggcactgggt cgctttcgta ctgttttttt
    24361 ctgcattgat tgatatacca gtcggaattc agataactca ggtttatcac ccgcacatcg
    24421 gttcttaccc cttctacttc ctgaagatac catagtggat aggtatcatt atcaccgttg
    24481 gtaaatagaa ttgcattcgg agccagccct gacagataat tgtaagccat ttcagaggcg
    24541 agcctttgac cgcttttgtt gtgatcggcg aaattttctg caagcatgag aaccggaacg
    24601 gcaagcaggc agacgcttgt agtaatgatt gctacacgaa gggtttgcca ttttttctta
    24661 agcagataaa aaagtgcata acagccaatg ccaatccaaa cagaaaatgt gtagaaggag
    24721 ccggcataag aataatccct ttcacggggc tgatagggga attggttgag atagatcaca
    24781 attgcaattc ctgtaaaaaa gaaaagtaac agcagaatgc ccgatgcttt tttatccttt
    24841 ctgaagtgaa acaccaaacc aatcagaccc accaacaacg ggataaaaaa atatgtattt
    24901 tttgcagggt ttttcaggat gctgtttgaa tccactgaag tttcacccaa ccgcaacctg
    24961 tcgataaagc ctatacccga aatccagttc ccgttaacag gcccgccatg tccctgaata
    25021 tcgttctgcc tgccaacaaa gttccacatg aaatatctga aatacatatg ccctatctgg
    25081 tagcgaaata aaaacctcag gttttccttc atcgagggaa ttttgtcccc attattgccc
    25141 gaccattctt tgtaggcttc tgtatggttt tctttgctgc tccacatacg tggaaaatac
    25201 cgggtgtcct gttttatata atttggctgc atctgatagc cggtaatcac atatttaccg
    25261 tttacaatgc gatgcaatgg cgaagcttca atgtagggtt tctggggatt aagcacagaa
    25321 ttgaaaaatg gacctttcag tagcggacga tcgccatatt gctccctttc gagatacgat
    25381 aacaggttat atgcctgcga aggatcagct tcgttgatag gcgttcctgc tgaagatcgc
    25441 agcacagtga gtgcatacga agaataccct gttagcagca ataaaaccat agtaagaatt
    25501 attctcaggc gttgcagctt tttgcggatg gcaataaata tagaaacagc aaaaatagca
    25561 tacagaatca aaaatccggt aatcattccg gaacctatgg gcaagtgaaa cacgttaaca
    25621 aataacacat cggtgtaggc aaccgcattg cttactccgg ggataagaag ataaagtatc
    25681 gcaaaaagaa gtgctatggc aaacccggcc aacagtagta tattctttag gttcggttta
    25741 tactttttaa agccaaaaac aaataccagg gcgggaatgg tcaacaaatt gagtaaatgg
    25801 gttcctatcg ataagcccat aagataggtt atcaggaaca gccacctttc cgctcccggt
    25861 ttttccgaga ttttttccca tttcaacata caccagaata ccgctgcggt aaggagtgag
    25921 gacaggctgt acacttcggc ctcggtagcc gattgccaga aactgtcgga aaatgcatat
    25981 gcaagagcac cgacagcact gcttccccat atcatgattt gttgtacgcg tgaaaattcc
    26041 ggagtgtttt taaccgtaat gcgctgcaac aacatcacaa tagtgaaata cagaaacagg
    26101 atggtaaatg ccgatgcaaa tgccgaaagc aaattcactg caaatgcagc ataactaccc
    26161 gatccgaaag caaatatcaa agtaaaaaga cgcgcaagca atacataaaa tggcgctccg
    26221 ggaggatgcc caacctgcag tttatagcca acggcaatcc actcgctgct atcccagaac
    26281 cccgccgatg gagcaagact gatcagatat accagcattg caacgagcca gatactccag
    26341 ccgataattt tatttgtttt tgtggaatta aacatccccg aactatttgt gcagctaagg
    26401 tataacaaat aatctattcg taaatacagc acttgtgaat taattgtcag gtggtattaa
    26461 acttttctgc ctgacgttat ttgtctgata aaatcgttat ttttgtaaat aataaaaact
    26521 aaagagtatg aaaacactgt tcacaattcc gtttattttt ctggctttgt tgggaactgc
    26581 ccagaatgtt accgagggag aagctatcat ttcgggggta aagcagaatg ccattattgc
    26641 tacaattccc aaagctgatg tagaggtgat atccaaagag tggaaaaatc tgatgaaaaa
    26701 atacgatgca gaggttacag gtaaggatga actgtttgct aacaatgccg ttattaagtc
    26761 gattaccgac aattctatgg acgtgtatgc acgtttcgag gtaaataagg acaaagacgt
    26821 tgtaatgagc gttgcattcg atctgggcgg tttttatatg aattcatcag cacaacccga
    26881 attatttaaa gaagctaaaa aattcgtgca gaaatttgct gagagtcagt cgaaatcggc
    26941 ctataaagag actgtgaaag cagagcaaaa agtgctctcc ggcatggaaa aagaaaagaa
    27001 aaagctggaa aaagccaacg aaaaactgga gaagaaaaat gaagatatgc gcaatcaaat
    27061 caaggaaaac tcccaggcaa tcaaagaaaa tcaggctcag atcgataata aaataaagga
    27121 aatcgaaacg caggaaaata aggtgaaggc cctgaaaaag gacaaaccct gattgcaggg
    27181 agctgttaat aatttcttat taataatcat attgccatgc gcccctgcag gtagttttct
    27241 tatttttgtg tcatctaaac taacaaaata atgaaatttg gtgttgtcat tttcccgggt
    27301 tccaattgcg atcacgatat gaaatactcc ctgcaggata tattgctgca ggatgtggtt
    27361 gaactgtggc ataaagacag cagcctgaaa aactgtgatg ctattgcaat tccgggtggc
    27421 ttttcgtttg gcgattattt gcgctcaggg gcgcttgccc gtttttcacc tatcatgacc
    27481 aaggtgatac agtttgccaa tagcggaggg tttgtttttg gtgtttgcaa cggatttcag
    27541 attctctgcg aaacggggct tcttcccggt gctttgctgc acaacacaaa tcaaaaattt
    27601 atctgccgca accaattcat aaaagcggag cataatcaat cggcaatgac caatctggtg
    27661 cctactaata aagcgcttaa aattcccatt gcccatggtg aaggtcgcta ttatgccccc
    27721 aacgatgtgt tgtccgatat ggtcaatcac aatcaaattg tttggcgcta ttgcgatgcc
    27781 aatgctgagc tatcagaaaa aagcaatccc aacggctccg taatgaatat tgcaggcatt
    27841 tgcaaccgta aaggaaatgt atttggcatg atgccccatc cggaacgcgc tgccgacccc
    27901 gaactgggca ataccgacgg agtactcatc ctccagtcga ttatcaatca ccttcagaaa
    27961 taaaaatgcc aaccggtgcc tcggaattat tcgtacaaaa acttcaacat tattactgat
    28021 atttccgatt atttatatct ttaatctttg tttaataatt cggatatgct cgttaaaata
    28081 tacgcaagcg cagttcaggg catcagtgct cgcctgatta ccattgaggt gaatgccagc
    28141 aaaggctatg cgtatcattt ggttggtctg cccgacaatg cagtgaaaga aagccagcat
    28201 cgtattgtaa gtgctctttc gaataacgaa ctgagatggc cgggaaagaa aatcattatt
    28261 aatatggctc ctgccgatgt ccgtaaagaa ggttccgctt acgatttgcc tcttgcaata
    28321 ggtgtgttgg cagcttcgca gcaaatcgtt gccgagaata ccgaccgctt tgttatcatg
    28381 ggagaattgt cgctcgatgg gtctgtgaaa ccggtgaaag gagccctgcc catagccatc
    28441 gacgcacaga aagccgggtt cgaatacctg atactccccg ttgaaaatgc acgcgaagct
    28501 gctgtggtta agggtataaa agtgctgggc gtgagccata ttaccgaggt tatcgatatt
    28561 ttcgataaac aggtatttcc tgaaccggtt tccgttgaca tcgaagccga atttgcccat
    28621 gccgaagata tgctgatgcc cgatttttcg gaagtgaaag ggcaggaaaa tgtgaaaaga
    28681 gcgctcgaaa tagctgcagc gggtggacat aacattattc tgataggacc tccgggagcg
    28741 ggtaaaacca tgcttgccaa acgtatgccg ggcattttgc cacccttgag tctggatgag
    28801 gcactcgaaa caacaaaaat tcattcggta gccggaaaaa tgaacgggaa ttgttcgctc
    28861 gttacacatc ggccatttcg ttcaccacat cataccattt cggatgtggc aatggttgga
    28921 ggaggctcgt atccccaacc aggcgagata tcgctggcac acaacggagt attgttcctc
    28981 gatgaattgc ccgaattcaa gcgcaccgta ttggaagtaa tgcggcagcc cctcgaagac
    29041 cgcatggtaa ctatttcgcg ggcaaaagtt actattgaat atccggcagc cttgatgctg
    29101 atagcctcga tgaatccctg cccctgcggg ttttacaacc accccgataa aaaatgcctc
    29161 tgtactccat cgatggtgca gaaatatttg tcacgcattt cgggtccgct gctcgatcgc
    29221 atcgatatac atctcgaagt agttcccgtt ccgttcgacc gactggctga gaaaaaaaac
    29281 ggagaaacga gtgccgaaat cagaaagcgg gtggtggaag cacggaaaat acagttggca
    29341 aggtttcagg gagccaaagg ggtgtattgc aatgcacaga tgtcgagccg ccagctacgc
    29401 aaatactgcc ccctcgacga aagctcgctg cgattattga aaacagccat ggaacgtctg
    29461 ggtttatcag cacgtgccta cgaccgtatc attaaagttt cgcggaccat tgccgatatc
    29521 gaaacgagtg aaaacattct tacatcgcac attgccgaag ccatacaata ccgaagcctc
    29581 gaccgggaag gttgggctgg atgagaaaaa tgggggtaga ataaatctct ttaatttagt
    29641 ttgcaatttt aactaaatgc tgatgcaaaa tatctgaata tagataaatg tcatctaatt
    29701 tattgatatc gtgtttaatc tcttttttgt tttcatcaaa aacaccgata gattttttat
    29761 cttctttgaa gtaaaatctg catattgttt gtcgaattga atcatcaagt aaaatgctaa
    29821 aaaagttttg gaaatctcgg tagaaaattc ttgaacaatc aatcttttca cgcaaaatag
    29881 atttcacaat gaaaaatgct tcaatctctt catcagttgt tacaacttta ttggcttcct
    29941 cggctttttt atctgcatca atcttagctt gttcaatctc tttagcattt tctttatcta
    30001 gggccgattt taatctttca tttataatgt cactaataag ctgattcgct gaccttttta
    30061 caatttctgt gaactgattt attatgcgct ctgtggcttg accactataa acttgtttta
    30121 caaaatatcg aacaaaattt gctgaaggat tattaaattc atttgttaaa atagttctga
    30181 tttcattaga gtacttcaat tcacttgctg aattgaaaat tgtattaata tcaaaatatg
    30241 acttatgaaa ttttttaagc tcattaatag cttgttcttt cattgaagta atatcaaact
    30301 ctagaaatgg tttatcatcc attttattag gttcgatcaa atccgtgtaa aatctgtact
    30361 gaatcccatt tgttagcatc ccgaatttgg ccttggaagt gtggaaatat ctaaatagtt
    30421 gggaattata tacgtctaaa ttttcacccc accatttaca ctcaattagt atttgcggaa
    30481 tattatcctt caaaatagca taatcaactt tttcaccttt ttttagacct aaatcagcgg
    30541 tgtattctgg aactacttca attgggttaa aaacatcgta gcccaatatt tgtataaaag
    30601 gcataataaa agcatttttt gttgcttctt cggtttgaat ttggttcttt agttttacga
    30661 ctctttcagc cagttgttta atagaatctt tgaaatccat aatgcctagt ttttgtattt
    30721 gttatttaat atttgtcatc aaaagtaata attttgttca aaaaaaataa aataaacaat
    30781 gatgttgaag tactgctgat gcgttaaatt ttagtcacta aataaatctc tttgttcttt
    30841 gacat
//
LOCUS       DAOOQP010000024        28090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_1967_length_28090_cov_6.171215, whole genome shotgun sequence.
ACCESSION   DAOOQP010000024 DAOOQP010000000
VERSION     DAOOQP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28090
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1967_length_28090_cov_6.171215"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1405
                     /locus_tag="PLK75_04275"
     CDS             146..1405
                     /locus_tag="PLK75_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY38494.1"
                     /translation="MTQLITKDLQYYKFGMYGFLKNLKFFEPFLILFLLEKGLMFWQI
                     GVVYSVREITRNIFEIPSGVVADALGRRRTLVMSFIVYCISFVILFFSKSYAIILVGM
                     GVFAFADAFRTGNNKAMIYDYLDIRGWRDQRIRYYGFTRSWSQTGSAVSSLIAAIIVI
                     LFHNYTTIFLASVVPYLLNAVLIYSYPQFLEGEIRKMHTDDIWKAFRKASKDMITVLR
                     NPLCWKAININSFHSGYHKAIKEYIQPVIVLAALTLPFYNNVPSEKREAFLVGVIYFI
                     IYLTTAVASRSAGSFSERLRSPERALYITLLLGLLSGMLAGLSLHLGLPWLCISFFLI
                     VYVMENLRKPIGVAGVANKSESTVMASVLSVNFQWESLVAAVIAPILGILVDITSVGL
                     GLGITSAIIILISMTSYFVTKLIRKPF"
     gene            complement(1437..2327)
                     /locus_tag="PLK75_04280"
     CDS             complement(1437..2327)
                     /locus_tag="PLK75_04280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY38495.1"
                     /translation="MMLLASDTWRDYELIDSGNHRKLERFGDYILSRPEPQALWQPGK
                     PPGEWDNLCHASFSNTGGDENGISDKGKWSIIKPISENWAIKYKHNSVDLSFRLSLGS
                     FKHVGVFPEQSVNWHYIYEHVKNISPENCRVLNLFAYTGGASLAALAAGAEVYHVDSV
                     KQVVSWAGENMRLSGLSGIHWVLEDAFTFVQREVKRGKKYQGIILDPPAYGRGPNGEK
                     WTISSGLLPLLQLCKQLLDRKNGFLVLNTYSLGFSSLISDNIIKDLFTSANSESGEIY
                     VSDKAGHKLPLGAFTRCCFK"
     gene            2451..3236
                     /locus_tag="PLK75_04285"
     CDS             2451..3236
                     /locus_tag="PLK75_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOY38496.1"
                     /translation="MITSLQNQQIKWLQQLEKPKVRKTESKFLIEGGKELVYAALSGY
                     VIESVFYCNDVVAAEQLVNLKAIVKNAEFTEISRSVFEKLAYREDSGGMIAVAHTKDF
                     ALRHLVIGTNSFVLVLESVEKPGNLGAILRTADAAGVDAVIVADPHTDIFNPNVVRSS
                     LGTLFTCKIGIGTNEEVWAFLKKNTCIIYGAALGTEQFYHQICYTGNCALIMGTESKG
                     LSKFWLDKSDMLVKIPMRGKIDSLNVSVSAAVICFEAMRQRGF"
     gene            3248..3721
                     /locus_tag="PLK75_04290"
     CDS             3248..3721
                     /locus_tag="PLK75_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOY38497.1"
                     /translation="MKTLIVFMSKHGTTEKAANLLKEQLGNNETDLVNLGKEKTPNIA
                     LYDTVIVGGSIHAGSIQKGIKKFCRENKQILLTKKTGYFISCMEKSKKQQEFEAAFDQ
                     ELRDKAVALGTFGGEFNFDKMNFFEKAIIKKISGFSSSVSDIDYDAIKEFASNFK"
     gene            complement(3730..5739)
                     /gene="dnaG"
                     /locus_tag="PLK75_04295"
     CDS             complement(3730..5739)
                     /gene="dnaG"
                     /locus_tag="PLK75_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOY38498.1"
                     /translation="MTIFAGLQAPENSTMISQVTIDKIIDTARIEDVVQDFVPLKRRG
                     VNLIGLCPFHKEKTPSFNVSPSKGIFKCFGCGKGGNAVNFIMEHEQLDWISAIKYLAK
                     KYRIEIEDRELTEEEKKLGSERESMLIVSAFAQKFFSRILNESEEGIAIGLSYFRERG
                     FNDTIIEKFQLGYCPEGKSIFTDEALKNGYKLEFLEKTGLSVVKDTHNYFDRFSGRVM
                     FPVFSISGRVIAFGGRTLKSDKNIAKYLNSPESAIYHKSQTLYGLYQAKTAIQQNDKC
                     ILVEGYTDVLALHQAGIENVVASSGTSLTKDQIQIIRRFTANVTILYDGDPAGIKASL
                     RGIDMLLEEGLNIKILLLPDNHDPDSFAKKHNYSFLVDYFKQHEQDFIHFKSKLLSDE
                     AKNDPVKRTYMISDIVRTISVIDDSIKRTVYLQECSRLLKIPEHTLFQETAKYRRKNI
                     EDKRKAEERAHLPESVPIAPPVPSFINEELYCEPYEKELIRLMLLYGEHSIFENLGDE
                     AYDEPHSTIKISEYIINEIKNDELEFKNLKYRKVFLHAEELIFRGITPTVEMFINNKD
                     PEISKVAADVCSPGYQLSAIWAKHHLNVEPEERHLDQVVPRVLVSYKRRIMEQHREEA
                     IKRLQELQHNPASETGEIENIQLRLTQLNSLINKCARLYNCVVIK"
     gene            complement(5831..8239)
                     /locus_tag="PLK75_04300"
     CDS             complement(5831..8239)
                     /locus_tag="PLK75_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38499.1"
                     /translation="MKQWIIVCFAVCIAFHINAQTVSIYDQVTHQPVPWATLQCSATG
                     NGVISDISGKADISAFRNCENIEIRCVGFATVTYSYQQLEQKGYKIYLAEDKISLNTV
                     VISANKWTQPRSEVPVKTLVIGKNDVLFQSPQTAADMISLSGGVFVQKSQLGGGSPMI
                     RGFATNRLLISVDGVRMNNAIFRSGNLQNIISVDPFVVDKAEVVFGPGSVIYGSDAIA
                     GTMNFFTYTPMLSITDEPYVKAGFTGRFSSANFEKTGHAHVNVGFRKFASFTSFSFTD
                     FDDLTMGAAGPEEYLRNSYAATINGSDTVLTNSNPRKQVGSAYSQYNLLQKFRFMPSE
                     KWDLNYSFYYSATSDYGRYDRLVRPKGNTLRSAEWFYGPQVWMMNHLAIFHNARTKIS
                     DKLAIHLSYQLFEESRFDRDLNKSIRHERFEKVHALTANIDLEKQFGEHHQLLYGADF
                     VYNIIRSSGKENNVFTGESEVGPSRYPDNSDWASMALYVSYRYKLNDKLSMQIAGRYN
                     YFMLDALFESTFYAFPFSEAHLSESAPTGSLGLVYSPSQSWQFIGNVSSGFRTPNIDD
                     IGKVFDSEPGSVIVPNPELKPEYAYNVDLSVIKTFGDFLKLDITGYYTYLDNAMVRRN
                     STLNGQDSIFYDGEMSQVQSLQNAAYATVYGVQGGIEIKLPFRLGISAFMNYQKGSEE
                     LDDGTTAPLRHAAPFFGSAHLTYTYNRVKLDFYSIFNSKVSYENLAPEEKSKDYIYAC
                     DENGNPYSPAWYTLNLKAMYLFSDNFSMSAGVENITNIRYRPYSSGICAPGRNFVVSV
                     MATF"
     gene            complement(8289..8840)
                     /locus_tag="PLK75_04305"
     CDS             complement(8289..8840)
                     /locus_tag="PLK75_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38500.1"
                     /translation="MSKKIVPILLLLILGIPIAGTYFYFKQRQYEIRKSIKRQIKKGA
                     SEQDLILLKIPKKLEDEPNADFERIHSKEFRYKGEMYDIVRSKNYETETWYWCIWDKE
                     ETELFASLNILFQRAWNSDPAQKQAQTHLSNFFNSLFLPCKPPDKKPGTIINFFYITR
                     NIIIHDLFSEKPPSPPPKNIVLT"
     gene            9011..10798
                     /gene="lepA"
                     /locus_tag="PLK75_04310"
     CDS             9011..10798
                     /gene="lepA"
                     /locus_tag="PLK75_04310"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOY38501.1"
                     /translation="MKHIRNFCIIAHIDHGKSTLADRLLQMTNTVSERDFQDQVLDDM
                     ELERERGITIKSHAIQMEYEFEGQQYILNLIDTPGHVDFSYEVSRSIAACEGALLIID
                     ATQGIQAQTISNLYQAIDNNLEIVPVMNKMDLDAAMPDEVEDQIIELIGCKSSDIIRA
                     SGKNGLGVEEILEAIIRRIPPPTGDTEKPLEALIFDSVFNPFRGIIAYFRIFNGIIST
                     KDLVKFMSTSQEYLAYEIGVLKMGLEARKQLRAGDVGYIISGIKTATEVKVGDTITHV
                     ERPCEMAIDGFSEVKPMLFAGIYPVDADDYEELRSSIEKLKLNDASLTFVPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFDMDVITTVPNVQYLAYTTKGEMIEVHNPSGMPEP
                     NYLEHVEEPMIKGHVISQAEYVGPIIKLCIDKRGTLLNQIYLTNNRVELSFDLPLGEI
                     VFDFYDKLKSISKGYASFDYFMDGYKVSKLARLDILLNGEMVDALSTLTHVDNAYPLG
                     RKMCEKLRELIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRMIGTVEVPQTAFLAVLKID"
     gene            11506..11727
                     /locus_tag="PLK75_04315"
     CDS             11506..11727
                     /locus_tag="PLK75_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38502.1"
                     /translation="MDIQAKKYLLIEWITSLSDASLIDKLIRIAEKSDWWDEISAAER
                     DSIEKGLKDITENRIVNHADVMKRYEKYL"
     gene            11711..12025
                     /locus_tag="PLK75_04320"
     CDS             11711..12025
                     /locus_tag="PLK75_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOY38503.1"
                     /translation="MKNTCKVVWSLEAVENLEAIIHWLEINWSEKEIKKFLIKLEKQI
                     QIIQSQPLSFPRSQVIAARKSVLSKHLTIYYSYTKECIYVLSIFDNRQALDKLSTKND
                     SY"
     gene            12230..13672
                     /gene="asnS"
                     /locus_tag="PLK75_04325"
     CDS             12230..13672
                     /gene="asnS"
                     /locus_tag="PLK75_04325"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOY38504.1"
                     /translation="MKRIKIKEILQKGNPGAEVRILGWVRTKRESKNVAFLAVNDGSG
                     MGNIQVVVEVNETTIPLLQRIHTGASVSADGLLIESTGSGQRVEVQCKKLMVIGEANP
                     DEYPLQPKKHSLEFLRDIAHLRFRTNTFGAVMRIRHAMIFAIHKFFNDKGFYNIHTPI
                     ITGSDAEGAGEMFRVSVLDAKNPPLTEEGKVDFSKDFFGRETNLTVSGQLEAELAALA
                     LSDVYTFGPTFRAENSNTARHLAEFWMIEPEMAFYDIDDNMDLAEEMLKYLVKYALDN
                     CREDLDFLASRRADEQKSKPENERDEMGLIEKLEFVLNNSFARITYGEAIDILMNSKP
                     NKKKQFQYPVEGWGTDLQSEHERFLVEKHFKKPVILTDYPKEIKAFYMKQNDDGKTVR
                     AMDVLFPQIGEIIGGSQREENYVKLLNRMHEMHIPEKEMWWYLETRKFGSVPHSGYGL
                     GFERLMLFVTGMSNIRDVIAFPRTPKNAEF"
     gene            13714..15168
                     /gene="rpoN"
                     /locus_tag="PLK75_04330"
     CDS             13714..15168
                     /gene="rpoN"
                     /locus_tag="PLK75_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOY38505.1"
                     /translation="MLKQSLQQKLLQKLSPQQIQMIKLLELPTLQLEQRIKKELEENP
                     ALEEGADEDENTDTNEDENEEQNENDDEFSLEDYIDDEDDIPAYRLQVNNFSKDDERK
                     EIPFSAGISFHENLKDQLGLKQISPRDRVLAEFLIGSMDEDGYIHRDLESIADDLAFN
                     QNISATTQEIENALYIVQDLDPPGIGARNLRECLLIQLDRKEKKTLPVLNAHEIIFSY
                     FEEFTKKHYDRIMQRLELSEPELKQALEMILRLNPKPGSSYSDSSVRSAQTIVPDFIL
                     ENYSGKLELSLNARNAPELRLSRDYSEMLQGLSKSKDSGSRSEKDAVVFIKQKLDSAK
                     WFIDAIKQRQNTLLITMNAILEYQKDFFIQGDETLLKPMILKDIAEKTGLDISTISRV
                     ANSKYIQTPFGNYLLKHFFSEAMQTDSGEEVSTREIKQILKECIENENKRRPLTDDKL
                     ASILKEKKYVIARRTVAKYREQLGIPVARLRKKL"
     gene            15168..15791
                     /locus_tag="PLK75_04335"
     CDS             15168..15791
                     /locus_tag="PLK75_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38506.1"
                     /translation="METGKFLNTIAKIISVLFHPLIIPTFGCIVIFNSGYYVGLLNPK
                     IMQAVYIVVFVLTFLLPALIIPALYLQKYITSFAIDEKNERLLPITIVAVMYGLSYYF
                     MQRSGFPPILLHFILGCIICLVLGLLITIKWKISLHMIGLGGLTGLIVFLAHSYGLGL
                     HYFLIFVILASGVTATARLILQSHNQLQVYAGYFLGVAGIWLSMIFL"
     gene            15867..16127
                     /locus_tag="PLK75_04340"
     CDS             15867..16127
                     /locus_tag="PLK75_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38507.1"
                     /translation="MPFIVKFILFLFLVYIIGKILLRYVFPYLIKWWLRRIAKKMNPE
                     AFEKPSGKNRKEGDITIKNMPNTKKNKGKSDEKGEYIDYEEL"
     gene            16215..16946
                     /locus_tag="PLK75_04345"
     CDS             16215..16946
                     /locus_tag="PLK75_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOY38508.1"
                     /translation="MKFIRKLLYKFLRIENYLRLVSSVYLLLIKAGFYKRKYPEIFFL
                     KQIIKEGDVCLDIGANLGYYSWFISKFCGKDGKLIAVEPVPLFGKIWLRNMKSSKFAN
                     FRLESCALGSENTRVKMATPEVNGVLHHGMTHVVNEGEAQQSTCYEVEMKIPDALFSA
                     LDRLNFVKIDVEGYEFIVFSNMVNTISKFHPVIQAELSGSENRLKVLALLESLYYKPH
                     VLENQQLRAVNQSDIETGISDFYFI"
     gene            17006..19084
                     /locus_tag="PLK75_04350"
     CDS             17006..19084
                     /locus_tag="PLK75_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38509.1"
                     /translation="MKKQLILFIALLAVTCILYIPALKNEFNDWDDAAYVTSNPNIEL
                     TKENIVRSFTQGEHHRMYAPITALSHSVVYTLFGLNPKPYILGNILIHLCNTLLLFLF
                     LSQLIKKPLVPLFAAALFALHPMQVESVAYVSGRRDVLYVFFYLISLIYYVKYATQGK
                     NYIVKYLLSLIFGVLALLSKGQALTLPFSLILISLFTGVKWNSKKFWLDKIPFLILTA
                     IFAVKVFSAPQFASGNYLSTAYLDSDIPFFYSIIYACYGFVQYIILLFFPYRLSLIHP
                     YPEVAGAYVIPYYMYIYIAILAGLIIAFFRFAVKRKLFWFGFTFFAINIAMLLQIIPN
                     SYGIMNDHYVYFSGIGIFLIMTDKITEVMNHRKWSTLIAILFSVYLVVLCGLSFQRIG
                     VFKDSIKLWSDVIKKYPDHYMAYSSRANANFNQGFFENAIIDYKRSIELNPENYALYV
                     NLGSAYSAAQQYKNAVIAFTKSLNHKPTSEAYTFRADAYSALGNTNDAIRDYSNAIEL
                     KPDYAVAYNNRGVVYEKMAETEKAMQDYKKAFDLDSTNIDACYNCAKLYGNNGNFEKA
                     LYYLDKLIVIKPDFAAAYNSKGIIFATTGSYEAAILCFSSAIENKPDYAEAYNNRGSV
                     YYRTGKFDLAIADYTRAAEIFPGFAETFNNRGAAYIALDKKDSACSDFTIAAQLGLDS
                     AKENLDKFCN"
     gene            complement(19159..20388)
                     /locus_tag="PLK75_04355"
     CDS             complement(19159..20388)
                     /locus_tag="PLK75_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOY38510.1"
                     /translation="MKPRQIKHLDFWVGIPLVFLLTLWRYLILLFVSRRRDIKTHRKI
                     IFIKLTEQGSTVLAYSALKEAEDMVGKENIYFWAFAENTPILEILNVFHEKNVFTIHN
                     KGFITTSFEILKTLAAIKKEKIDTAIDLEFFSRSSAILAYCCGAKTRVGYQRYHSEVI
                     YRGDLMTHKLQYNPYHHAALAYRLMVESLLCNHKIRPFPRIDINTIQILQPIIEITRD
                     DVAEIKKIIHSVSQEENISPIIIINPNAGDSIPARKWDVSNFVDLSNKLSSHYKGKLT
                     IVITGSLEEATYCNELCDKINFPGVINLAGKTNVRQLLALYSLADVLVTNDSGPGQFA
                     SLTSIHNIVLFGPETPVLFGPPGKNTHIIESDLSCRPCVNPYNHRLPHCKSNTCMQSI
                     SVETVYDKIIEVIPVYS"
     gene            complement(20520..21413)
                     /locus_tag="PLK75_04360"
     CDS             complement(20520..21413)
                     /locus_tag="PLK75_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY38511.1"
                     /translation="MKNQSKAYIYAVLTVLLWSTVATAFKIALKQFDFINFVCFSSLT
                     AALVLLVIIVIGGKWRELMRTTGRDLIKSAMLGFLNPFAYYLVLFKAYDILPAQEAQP
                     LNYVWPIMLVLISIPMLKQKISWMSLLAIIISFVGVLVISTHGNLLEMKFTNGTGAAL
                     ALGSSLIWAFFWIFNMKDKRDETIKLFLSFAFGFVFSMIACIIFSKPSFNFTVSALAV
                     VYSGLFEMGITFLLWSKALKYSSTTAKVSNFIFASPFLSLIGIYFVVGEKIMESTIIG
                     LVLIVSGIVLQQYAGRKVILK"
     gene            complement(21416..23191)
                     /locus_tag="PLK75_04365"
     CDS             complement(21416..23191)
                     /locus_tag="PLK75_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOY38512.1"
                     /translation="MSRKIFLFTIFIVVGLPIAFGQKLIVTPYEHSEKTATMTYDEIL
                     HYSKLFDSTCNYIKYISIGKTGNGYDIPLLIYDASAKFDPIAARKAGKSVLLIQAGIH
                     AGEPDGTDAGMMLFRDIACRKIRPPENLIILFFPTINPEGCLRRSAYNRINQNGPKEM
                     GWRTNAANKNLNRDYVKAETPEIRCFLEVFNTWKPDMFIDCHTTDGADYQYVMTYDLT
                     DMCLLSDAQNEWQDKVYMQPLLKRMREKDFPMFRYVTFKNWHDPKSGLVRWIPSPVLS
                     EGYAAARNRPGLLLETHMLKPYGKRVDATYEVLKYTASLVDTQHEIIIKLNAESDLAV
                     SKLYKNKKPVSVDFNSLADSVQIDFEGVEYTVVKSLITGGNWFQYSDTPAIHKVWFFN
                     NPRPSIQTTLPAAYVFEPVWGNVTAVLDAQGVKYIRLYKEMKVPVEAYRLKNVKFASE
                     PYEGKQRIIDFATEVTYDSLVFPAGSIIVETSNPYARLLAYMFEPVSPASLVRWGFFN
                     AVFEQKEYAESYVMEAMVISMLKSDPFLNTEFNDKMMNDKEFASNPRAILNWFYQKTV
                     YWDRKKDIVPVYRVDDATLRKLLSK"
     gene            23367..23843
                     /locus_tag="PLK75_04370"
     CDS             23367..23843
                     /locus_tag="PLK75_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38513.1"
                     /translation="MKILKIRHLILVVAITIIAILACSKNEPISQGTSINLRYNGDDV
                     PMNFAWYQCQCTTQNGTVYSGQRCEVTIMIPNCTRQDAELGCICKTRDKVIYDKTSKL
                     TRNADLTISELDSVIEILNSEPITKQVISNHYRVFYFLYTEGILEHPDTLIKYSIL"
     gene            23854..25068
                     /locus_tag="PLK75_04375"
     CDS             23854..25068
                     /locus_tag="PLK75_04375"
                     /inference="COORDINATES: protein motif:HMM:NF025336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4221 family protein"
                     /protein_id="HOY38514.1"
                     /translation="MRLINQYITVISFIAPLVIYTMIFWGCHHEETMKFSFHVGTDSL
                     ILKNFTGGVGSVKFLEQGDSVFVFMLNSAGDKFCRVSYTPYLTTDISVVSIPTQLREN
                     KVAGIQQARSILPISTDSIMFAQKTDDYGFIFSLYVMSTGTIQPIYISKPENRIFFQE
                     LPEDGINWKYDKKLRKIIVAIVRQDDYDVRPYPIGTEALAGIDIATGEFEILPLKYPV
                     NYEEKYQSNISRSEYFCLNDSLVVLTFAMHDTAFIYNTTTGICSKKCMASKSDRQFLT
                     ISQDDYENCETEKLRDANLRSFKYRSVFYDPYRKMYFRFYEQFMPAKNNDGLFNIWSD
                     KQIGFACFDQEFNYIGEDCFGKMGDADVMFIATGQPVRRGMLLFLNYNQTQGSVTANN
                     LLRLMLIQVETN"
     gene            25103..25498
                     /locus_tag="PLK75_04380"
     CDS             25103..25498
                     /locus_tag="PLK75_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38515.1"
                     /translation="MALSCHDKPHYEREKELISYLKTKHPEVLQQDRCGLLVYRKPYR
                     CDLFFDAGLVNDINNVLYSQKPEKLYVIYDFETTKDSLETNINFENKVYFREDYNCLD
                     RYGFRLAPMFFIIKNGRIDYWHTITKGCD"
     gene            complement(25575..26948)
                     /locus_tag="PLK75_04385"
     CDS             complement(25575..26948)
                     /locus_tag="PLK75_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38516.1"
                     /translation="MDKYNIQSYYHRFRRLTPWGRKLKHHCFALRSNVYFHINGDVSM
                     CSHNHSLVLGKFPENRISEIVEGEFRRQATESFLSGDIPTDCRSCYEKLKSNNPKAAT
                     FQVYNQAYRKSGIPLTAEFELSNHCNLACIMCSPVLSDQIHSEKKPDLPSPYNTDFVE
                     QLTPYLAYLKRASFKGGEPFLIDLYFEIWEKIIASGAKTIISVTSNGTVLNKKVRDIL
                     ERGRFDINISIDSLEKDKYEEIRRNASFDVFRRNLDFFIGYCKSKKTNFNSCTCLMTN
                     NYSGVPDIYRFAIKNRFNIFMNYVEHPAYLSVKTMEKNKILEVMTYMQKHMPKFSPIR
                     DRENRASYDAMIRQLELWAHEKEPDVTIPSQAIPEITNTEQVALLLNKLRQHFATYYG
                     ISGDVETNSDYLMQLNKFTTAYQKASAQVLSKNIIASLIACSEERFVALFSLSEEELI
                     EFLCHDV"
     gene            27163..>28090
                     /locus_tag="PLK75_04390"
     CDS             27163..>28090
                     /locus_tag="PLK75_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38517.1"
                     /translation="MNYKNSKIVSILTSFGFWGIFLIVALAVQMILFMLFSYQSICIA
                     SGVLFWAIPWWLTTIIMRHNSQKLKTAIHSVFGTLMLVEVVLRASGLVANYWERQHGY
                     YKSVYDVDSSVFVQKRTPGVYQISNRDCSFEITTNSWGYRDKEWTPENMKSKIRIMAL
                     GDSFTEGYGAPADSTWMNLLEKEIADTGYYFINGGMIGSDPVADYNRLKTNLIELDPD
                     IILFAINITDLSDLMLRGGPERLSNSSVEKLKAPWWELIYAMSHTLRLGINQFYEASL
                     LISHKQVAVKQEKYLLIMEDFCKNVTNEFNDSI"
BASE COUNT         8440 a         5343 c         5823 g         8484 t
ORIGIN      
        1 tataaacttt ttgttgttaa ttatcatatt attagtataa ttcaaaacag tttctaaata
       61 tcagggcaaa agaaatgtta tttttacctt gggtaattga ttccggtatt tagagcaatt
      121 ttgattttac tcgtgtaagc cagtcatgac tcagttgatt actaaagatt tacaatacta
      181 taaattcgga atgtatggtt ttttgaaaaa cctgaaattc tttgaacctt ttttaatttt
      241 atttcttctc gaaaaagggc tgatgttttg gcagattggc gtggtgtatt ctgtgcgcga
      301 aattacccgt aatatttttg aaattcctag cggtgttgta gccgacgcgt tgggacgccg
      361 ccgaacattg gtgatgtcgt ttattgtgta ttgtatttca ttcgttattc tattttttag
      421 taaatcgtac gctattattc ttgttggtat gggcgtattt gcctttgccg atgctttccg
      481 aacggggaat aataaagcca tgatttacga ttatctcgat attagaggtt ggagagacca
      541 gcgtatacga tattacgggt tcacaaggtc gtggtcgcag acaggttctg ctgtcagttc
      601 actgattgcg gcaatcatcg taatactatt tcataactac acaaccattt ttctagcctc
      661 tgtggttcct tatttgttaa atgcagttct tatttattca tatcctcaat ttctggaagg
      721 cgaaatcagg aaaatgcaca ctgacgatat ttggaaagcc ttcaggaaag cctctaaaga
      781 tatgattact gtgcttcgta atccattatg ctggaaggca attaatatca actcttttca
      841 ttcgggttac cataaggcta tcaaggagta tattcagccg gtcattgttt tggcagcact
      901 tacattacct ttctacaaca acgttccatc ggaaaaaaga gaggcttttt tggttggcgt
      961 tatttatttt ataatatacc ttactacggc tgttgcctca agaagtgccg gttcgttttc
     1021 cgaacgatta cgttctcccg aaagggcttt atatattacc ctgttgcttg gtttgcttag
     1081 tggtatgttg gctggactga gcctgcatct aggcttacct tggctgtgca tatcgttttt
     1141 ccttatagta tatgttatgg aaaacctgcg caaacctatt ggagtggccg gcgtagccaa
     1201 caagtcagag tcgacagtga tggcatcggt attatcggta aattttcagt gggagtcgct
     1261 ggttgcagct gtgattgcac caatattggg aattttggtt gatatcacga gtgttggact
     1321 gggtttgggc attacttcag caataattat actgatttct atgacaagct actttgttac
     1381 caaattaatt agaaagcctt tctgagaata tttctggagg aaaaatgaac acctgtttat
     1441 ttgaaacagc atcttgtgaa tgctcccagc ggaagtttat ggcctgcttt atcactaaca
     1501 taaatttcac cgctttcgga gttagcactt gtgaataaat ctttaattat attatcgcta
     1561 atcagcgaag agaacccaag tgaataggtg ttaagaacca gaaacccatt ttttctgtcg
     1621 agaagttgtt tgcaaagttg aagtaaggga agtaagcctg atgaaatggt ccatttttca
     1681 ccgttcggac ctctcccgta tgccggaggg tcgaggataa ttccctgata ttttttgcct
     1741 cttttcactt cgcgttgcac aaaagtaaat gcgtcctcaa gaacccagtg aattcccgac
     1801 aaaccggaaa gcctcatgtt ttcacctgcc cacgaaacta cctgcttcac tgaatcgaca
     1861 tgataaactt ctgctccggc agctaatgct gcaagcgatg caccaccggt gtatgcaaaa
     1921 aggttcagaa cccggcagtt ttccggtgat atatttttta catgctcgta tatgtagtgc
     1981 caattcaccg attgttccgg gaaaacgcct acatgtttaa agctacccag actcagtctg
     2041 aagctgaggt cgacactatt atgcttatat ttgatagccc agttctcgga tatcggtttt
     2101 ataatactcc atttaccttt atccgaaatc ccattttcat ctcctcctgt gttgctgaag
     2161 ctggcatggc acaagttgtc ccattctccg ggtggtttcc ctggttgcca cagtgcctgt
     2221 ggttcaggtc tcgaaagaat gtagtcgccg aatctttcaa gtttacggtg attcccgctg
     2281 tcgataagct catagtcgcg ccaggtgtca gaggcaagaa gcatcatgct ggtaagtttt
     2341 atgcaaatat ctgcaaatac aatggttcag acaaatttat ttaatacgct taattggggt
     2401 catcattttg tctaaatatt gttttacatt atatttgaag aaaaatcact gtgattacaa
     2461 gtcttcagaa ccaacagatt aagtggctgc agcagctcga aaagcccaaa gttcgcaaaa
     2521 cggaatcaaa atttctgatt gagggaggta aagaacttgt ctatgcggct ttgtcgggtt
     2581 atgtaatcga aagtgttttt tattgcaacg atgtagttgc agcagaacag ctcgttaatt
     2641 tgaaagcgat tgtaaagaat gctgaattta ccgaaatctc aagaagtgtt ttcgaaaaac
     2701 ttgcctatcg cgaagattcg gggggtatga ttgctgttgc ccatacaaag gactttgcat
     2761 tgcggcacct tgtaatcgga accaactctt ttgtacttgt attggaatca gtagaaaagc
     2821 ctggtaatct gggggcaatt ctccgaacag cggatgcagc cggggtggat gccgtgattg
     2881 tggcagaccc tcataccgat attttcaatc ccaatgtggt acgatcgagt ttggggactt
     2941 tatttacatg taaaattgga attggaacga atgaagaggt ttgggcattt cttaaaaaaa
     3001 acacatgcat tatttatgga gctgcattag ggactgaaca attttaccat cagatttgtt
     3061 ataccggcaa ctgtgcgtta attatgggaa ctgaatcaaa aggtctgagc aaattctggc
     3121 ttgataaatc ggatatgttg gtaaaaattc caatgcgcgg taaaatcgac tcattgaatg
     3181 tttcggtttc ggcagcggtt atttgttttg aggctatgcg tcaaagaggt ttttaataca
     3241 ttaaaatatg aaaacactta tcgtatttat gagtaaacat ggcacaactg aaaaggctgc
     3301 caatttgttg aaagaacaac tgggtaacaa cgaaaccgac ctggttaacc ttggtaaaga
     3361 gaaaactccg aatattgcgc tttacgacac tgtaattgtg ggaggatcga ttcatgccgg
     3421 gtcaattcaa aaaggtatta aaaagttttg cagagagaat aagcaaatac tactcactaa
     3481 aaaaaccggg tattttatta gttgtatgga aaagtcgaaa aaacaacagg agtttgaagc
     3541 agctttcgac caggaattgc gcgacaaggc tgttgcactg ggaacattcg gaggagaatt
     3601 caatttcgat aaaatgaatt ttttcgaaaa ggccatcata aaaaaaatat cggggttttc
     3661 gagtagcgtt tccgatatcg actacgatgc aattaaagaa tttgcgtcga attttaaata
     3721 gaggtgtaat cactttatta ccacacagtt atatagcctc gcgcacttgt ttatcaatga
     3781 gttaagttgg gttaaccgga gttgtatgtt ttcaatctcg cctgtttctg acgcgggatt
     3841 atgctgtaat tcctgaagtc gtttaattgc ctcttcacgg tgttgttcca taatacgtcg
     3901 tttgtagctt acaagtactc gtggaacgac ctgatcaaga tgcctttctt caggttcaac
     3961 attcagatgg tgctttgccc atattgcact tagctggtat ccgggcgaac acacatctgc
     4021 agccaccttg ctgatttccg ggtccttgtt gttgataaac atttcaacag tcggagttat
     4081 tccgcgaaag atcaattctt cagcatgaag aaatactttc cggtatttca gatttttaaa
     4141 ctccagctca tcgttcttaa tttcattgat gatgtactct gaaattttaa ttgtactgtg
     4201 aggttcatca taggcttcat ccccaaggtt ttcgaaaata gaatgttctc cgtaaagcaa
     4261 cataagcctg atcagttctt tttcgtaagg ctcgcaatac aactcttcat taataaatga
     4321 aggaaccggg ggcgcgatag ggacgctttc cggcaggtgt gcccgttctt cggcttttcg
     4381 tttatcttcg atgtttttac gacgatactt tgctgtttcc tgaaacaggg tatgctcggg
     4441 tattttaagc aaccggctgc attcctgcag gtagactgtt cgtttaatgc tgtcgtcgat
     4501 caccgaaatg gttcgaacaa tgtccgaaat catatatgta cgcttgacag gatcattttt
     4561 agcttcatct gataaaagct tcgatttaaa atgtatgaag tcctgctcgt gctgtttaaa
     4621 atagtcaacc agaaacgagt aattgtgttt ttttgcaaaa gaatcggggt cgtggttatc
     4681 gggtaaaaga agtattttaa tgtttaaacc ctcttcgaga agcatatcga taccgcgcaa
     4741 cgaagcttta ataccggcag gatcaccgtc gtaaagtatt gtaacatttg cagtgaaccg
     4801 cctgataatt tgaatctgat cttttgtcag agaagttccc gaagaggcca ccacattttc
     4861 gatacctgcc tgatgcaatg ccagaacatc ggtatatcct tcaacaagaa tgcacttgtc
     4921 gttttgttgt atggcagttt ttgcctgata aagtccgtac agggtctgtg atttatggta
     4981 gatggccgac tccggggagt ttaggtattt tgctatgttt ttatcactct tcaatgtgcg
     5041 ccccccaaag gcaattaccc tgcccgaaat actgaacaca ggaaacatta cccttccgct
     5101 gaaacgatca aaataattat gagtgtcttt aacaaccgat aatccggttt tttcgagaaa
     5161 ttcaagcttg taaccgtttt tcagggcttc atcggtaaat atacttttcc cttcggggca
     5221 atagccaagc tgaaatttct ctataattgt gtcgttaaat cctctttctc tgaaatagct
     5281 taatccaatg gcaattcctt cctccgattc attcagtatg cgtgagaaga atttttgtgc
     5341 aaaagcactg acaatgagca tgctttcacg ctcactgccc agtttttttt cttcttcggt
     5401 gagttccctg tcttcaattt cgatccggta tttctttgcg agatatttaa tagcactgat
     5461 ccagtcgagc tgttcgtgct ccatgataaa atttacagca ttccctcctt ttccgcaccc
     5521 aaagcattta aatattcctt tcgaaggact tacattgaag gatggggttt tttccttgtg
     5581 aaacgggcac agaccaataa gatttacccc cctccgtttc agtggaacga aatcttgcac
     5641 tacatcttca attcttgcag tatcaataat tttatctatg gtaacctgac ttatcatggt
     5701 gctattttcg ggcgcctgta accctgcgaa gatagtcatt ttaaaaacgt aagacacccc
     5761 atttttcggt gcaacttaaa aaaaagcggc tacctgctaa ggaagccgct aaattttaaa
     5821 agattttaat tcagaatgtt gccattacag aaactacgaa attacgcccg ggagcacaaa
     5881 tgcctgagga atacggacgg tatcgaatat tggtaatgtt ttcaacacca gcgctcattg
     5941 aaaaattatc ggaaaacagg tacattgctt ttaaattaag ggtgtaccat gccggagagt
     6001 agggattccc attttcatca caagcataaa tgtaatcttt gctcttctct tcaggagcca
     6061 gattctcata actgactttg ctgttgaaaa tagagtagaa atcaagcttc accctgttgt
     6121 aagtataggt gagatgtgcc gatccaaaaa acggagcagc atgccttagt ggggctgtag
     6181 ttccatcgtc cagttcttct gaccctttct gataattcat aaaagccgaa ataccaagcc
     6241 tgaacgggag ttttatttct atacccccct gaacaccata aacagttgca taggcagcat
     6301 tttgcaatga ttgaacctga ctcatttcgc cgtcataaaa aatgctgtct tgtccgttga
     6361 gagtagaatt tcttctgacc attgcattat caaggtatgt gtaataaccg gtaatatcaa
     6421 gcttcaggaa atcgccaaag gttttaatca cagagaggtc aacattatag gcatactccg
     6481 gttttaactc tggatttgga actataacgc tgccgggttc cgaatcgaat accttaccaa
     6541 tgtcatcgat attcggagtc ctgaaaccgg atgatacatt ccctataaat tgccatgact
     6601 gtgagggact atacaccagt ccgagactcc ctgtgggagc actttctgac agatgtgctt
     6661 cgctaaatgg aaacgcatag aatgtagatt cgaaaagtgc atcgagcatg aagtagttgt
     6721 atcggccggc tatctgcatg ctaagcttgt cgttgagctt atacctgtaa cttacataca
     6781 aagccatcga tgcccaatcg gagttatcgg gatagcgcga aggcccgact tcggattctc
     6841 ctgtgaaaac attgttttct ttaccgctag atctgattat attgtaaaca aaatcggctc
     6901 cgtacaacag ttgatggtgt tcgccgaact gcttctcgag atcgatattc gcagtaagcg
     6961 catgtacttt ttcgaaacgc tcatggcgaa tacttttgtt caaatcacga tcgaagcggc
     7021 tttcttcaaa cagttgataa ctcaggtgaa tggcgagttt atcactgatt tttgtccgtg
     7081 cattgtgaaa gattgccaaa tgattcatca tccatacctg aggtccgtag aaccattcag
     7141 cagaacgcaa tgtattcccc tttggtctga ccagcctgtc gtatctacca taatcgctgg
     7201 ttgctgaata gtagaaacta taatttaaat cccatttttc ggaaggcata aagcgaaatt
     7261 tttgcaatag gttgtactga ctgtaagcac ttcccacctg tttacgtgga ttgctgttcg
     7321 tcaatacggt gtcggagccg ttaatggttg ctgcatagct gttacgcaga tactcctccg
     7381 gtccagccgc acccattgtc aggtcatcga aatcggtaaa actgaagctt gtaaaggaag
     7441 caaacttccg aaaccctacg ttaacgtggg catgtccggt tttttcgaaa ttggccgatg
     7501 aaaaacgacc cgtaaaaccc gcttttacgt aaggctcatc tgttatcgag agcataggag
     7561 tgtaggtgaa aaaattcata gtgccggcaa tggcatcact accgtaaatc accgacccag
     7621 gtccaaaaac cacctcggcc ttatcgacca caaatggatc tactgaaata atgttctgaa
     7681 gatttccgct tcggaaaatg gcattattca ttcgcactcc gtctaccgat attaaaagcc
     7741 ggtttgttgc aaaacctctg atcattggac tacctcctcc cagttggctc ttctgcacaa
     7801 aaacaccccc gcttaacgaa atcatgtcag ctgcggtttg ggggctctga aacagtacat
     7861 cgtttttgcc tataactaag gtttttacag gtacttcgct tcgtggttgt gtccatttgt
     7921 tggctgaaat tacaactgta ttcagcgata tcttatcttc ggcaagatat attttatatc
     7981 ctttttgttc cagttgttga tagctgtatg taactgttgc aaaaccaaca catctgattt
     8041 cgatgttttc gcaattgcga aatgccgaaa tatcagcttt cccgctgata tctgagataa
     8101 caccattccc cgtggcgcta cactgaagtg tagcccatgg aacaggttgg tgtgttacct
     8161 gatcgtaaat gcttactgtt tgagcattga tatgaaatgc aatacaaaca gcaaaacaaa
     8221 caattatcca ttgtttcatt gatgaaaaaa aattaaatta taaataagtt attaaagtac
     8281 tctgtttact atgtaagtac tatatttttg ggtgggggag aaggtggttt ttcgctaaac
     8341 aaatcgtgga taattatatt tctggtaata taaaagaagt ttattattgt acctggtttt
     8401 ttatcaggtg gcttacaggg taaaaataag gaattgaaaa aattactcag gtgggtctgt
     8461 gcctgtttct gcgcgggatc agaattccat gctctctgga atagtatatt taggcttgca
     8521 aaaagctcag tttcttcttt gtcccagata caccagtacc atgtttcggt ttcgtaattc
     8581 ttacttcgga cgatatcgta catttcgccc ttgtaacgga attcttttga gtgaattcgc
     8641 tcaaaatcgg cattgggctc atcttcaagt tttttgggaa tcttcagcag tatcaggtcc
     8701 tgctcactgg ctcctttttt aatttgcctt tttatagatt ttcgaatttc gtactgtcgc
     8761 tgtttaaaat aaaagtaagt ccctgctatt ggaataccaa gaatcagcaa caataatatg
     8821 gggactattt tttttgacaa aaccgaagat aaaagccaca aaccgatatc ctgttacaaa
     8881 gctactatat ttattactta atgcccagca tgagttcaaa tattttcaat atctcactat
     8941 ttgagcttgt gggtttgaca gggttgaaca tgaataagat tttatatatt tgcggcgaaa
     9001 cattacacac atgaagcaca ttcggaattt ttgcatcata gcacatatcg atcatggtaa
     9061 aagtacctta gccgacaggc tgttgcagat gacaaatacc gtttcggaac gcgactttca
     9121 ggatcaggtt ctcgatgata tggaattgga gcgggaaaga ggaattacta ttaagagcca
     9181 tgccattcaa atggagtacg aattcgaagg ccagcaatat atactcaact tgatagatac
     9241 tccggggcat gtcgatttct cctatgaagt gagccgatca attgctgcct gcgagggagc
     9301 attgcttata attgatgcca ctcaggggat acaggctcaa acaatatcga acctgtatca
     9361 ggctatcgac aataaccttg aaattgttcc cgtgatgaac aaaatggatc ttgatgccgc
     9421 tatgcccgac gaagtggaag atcagattat tgaacttatc ggttgtaaaa gtagcgacat
     9481 tataagagca agcggtaaaa acggtctggg tgtggaagaa attttagaag cgatcatacg
     9541 ccgcataccc ccaccgaccg gtgacactga gaaaccgctc gaagcactga tctttgactc
     9601 agttttcaat ccgtttcgag gtattatcgc ttatttcaga atatttaacg ggatcatttc
     9661 cacaaaagac cttgttaaat ttatgagtac atctcaggaa tatcttgctt acgaaattgg
     9721 tgttttgaaa atgggactcg aagcacggaa acaattacgc gccggcgatg taggttacat
     9781 aatttcggga atcaaaactg ccactgaggt taaagttggt gatacaataa ctcatgttga
     9841 aagaccctgc gaaatggcaa ttgatggttt ttctgaggtt aaacccatgc tttttgcggg
     9901 tatttatccg gttgatgccg acgattatga agaacttcgc tcatccatcg aaaaacttaa
     9961 actcaacgat gcctccctta cctttgtccc ggagtcgtcg gctgcactgg gctttggctt
    10021 ccgttgcgga tttctcggat tacttcatat ggaaattgtt caggagcgcc tcgacaggga
    10081 atttgatatg gatgtgataa ctactgtccc caacgtacaa tatcttgcct atactaccaa
    10141 gggcgaaatg attgaagttc acaacccatc aggaatgccc gaaccaaact atcttgaaca
    10201 tgtagaagaa cccatgataa aaggtcacgt gatttctcag gcagaatatg tgggtccaat
    10261 tatcaaactg tgtatcgata aacgtggtac cttgctcaat cagatttatc ttaccaataa
    10321 cagggtagaa ctatctttcg acctgccgct aggtgaaatt gttttcgatt tttacgataa
    10381 acttaaaagc atcagcaaag ggtatgcttc gttcgactat tttatggatg gatacaaagt
    10441 ttcaaaattg gcacgtctcg atattttgct taatggagaa atggtggatg cactgtcgac
    10501 tctgacgcat gtggataacg cctatccgct ggggagaaaa atgtgcgaaa aactcaggga
    10561 gctaataccg cgtcagcaat tcgatattgc tatacaagca gctataggag ctaaaattat
    10621 tgcgcgcgaa acggtaaagg ctgttcgcaa agatgttacc gccaaatgct acggcggcga
    10681 tattacccgc aaacgaaaac tgctcgaaaa acagaagaaa ggcaagaagc gtatgcgaat
    10741 gattggaacg gttgaagttc cgcaaacggc ttttctagct gtgcttaaaa tagactaaat
    10801 tcctgaacat ttcctggcag atatataggt gaacattgcg gtagtttttt gccaagaatt
    10861 caagttttcg aattaaccca aaatcagcaa taggaaatta agcaaattta acttattgat
    10921 tatcaataaa taaactcatt gtttttctaa tgcgtagtat tagagatttt tcaataaaaa
    10981 attcactaaa accctttacg cacaagaaat atacgataat tgcgaaagca tttttagttc
    11041 ctattgcgta atttgcgtta gttcagagaa ataattgcga acgattttta caaaatcaag
    11101 agcttaagaa gctgagccta ttcaggtcta aatatttcct tataattctg tagtttcatt
    11161 tctaaaaatt ttccaagcat ttttagaacc acgtttttgt gtgacagatt gattttatac
    11221 gctataaaaa gcatgttagg tgcaagtggt aagacgaagt gattaatcta atcaaaagct
    11281 tgttttctca cccataataa tgaaacaata ttctttcggc aaataataca attgatgtat
    11341 aaagaggaga aaggaaaaag aaacggtgca tgacctttgg aggtaacaga aacctcgagt
    11401 tggcaacaat ttattattct tcgtccagtg gagcttttaa aggcagcata agtttttgat
    11461 ttccgggata ttgaaataat ttgtaatttt gttaaaaagt caaacatgga catacaagca
    11521 aagaaatatt tactgataga gtggattacc agcctaagcg atgctagttt aattgataag
    11581 ttaatacgaa ttgccgaaaa atcggactgg tgggatgaaa ttagcgctgc agaaagagat
    11641 tcgattgaaa aagggctgaa agacattaca gaaaaccgta ttgtcaatca cgctgatgta
    11701 atgaaacggt atgaaaaata cctgtaaagt tgtttggtcg cttgaggccg tagagaacct
    11761 tgaggcaata atacactggc tcgaaataaa ctggtcggaa aaagaaataa agaagttttt
    11821 aataaaactt gaaaaacaaa tacagataat acagagccaa cccctctcct tcccaagatc
    11881 tcaagtgatt gccgcaagaa agtcggtatt atcgaaacat ttaacaatct actacagcta
    11941 cacaaaagag tgcatttacg tgttaagcat ttttgacaac cgacaagctc tagataaact
    12001 aagcacaaag aatgactcat attaactaaa gaaatatttg ctttgatacg gggaaacaag
    12061 ctaataccac cggcacctaa aaacagtttg tttttcataa gataaatcat aaatttgaaa
    12121 gcagtttact ccataagggc attgggcgag cagtgggtag ctgcaaaatg ttgttagtac
    12181 catgataaaa ttgtttttgc tatttttaca agctcaaaaa tcaaacgaaa tgaaacggat
    12241 aaaaattaaa gagatactgc agaaagggaa tcccggagcg gaggtaagaa tattgggttg
    12301 ggtgcgtaca aagagagaga gtaaaaatgt tgcttttctt gcagtgaacg atggttctgg
    12361 aatggggaat attcaggttg tggttgaagt gaatgaaact accatcccct tgctgcaacg
    12421 cattcatacc ggagcctctg tgtcggcaga tggactgctt atcgagtcga ccggcagtgg
    12481 acaacgggtg gaagttcagt gtaagaaact tatggtgatt ggcgaggcca accctgacga
    12541 atatccgttg cagcctaaga aacacagtct tgagttttta cgcgacatcg cccatttacg
    12601 tttccgcaca aacacttttg gtgctgtgat gcgcattcgc catgctatga tttttgccat
    12661 ccataaattt ttcaacgaca aggggtttta caatattcac actcccatta ttacgggcag
    12721 cgatgccgaa ggagcgggtg aaatgttcag ggtaagtgtt ttggatgcaa agaacccccc
    12781 gctcaccgaa gaaggaaaag ttgatttctc gaaagacttt ttcggacgcg aaaccaacct
    12841 gactgtatcg ggtcagttgg aggccgagct tgctgccctt gcactaagcg atgtgtacac
    12901 tttcggacca actttccgtg ccgaaaattc caatacagcg cgccatctgg cagaattctg
    12961 gatgattgaa cccgaaatgg ccttttacga catcgacgac aacatggatc tggccgaaga
    13021 aatgttgaaa taccttgtga agtatgcact ggataattgc cgtgaagacc ttgattttct
    13081 tgcttccaga cgggcagatg agcagaagtc aaaaccagaa aatgaaagag acgaaatggg
    13141 gctgattgaa aaactggagt tcgtgctaaa taatagtttt gcaaggatca cctacggaga
    13201 agccattgac attctcatga attcgaagcc aaacaagaaa aagcagtttc agtatccagt
    13261 agaagggtgg ggaactgatc tgcaaagcga gcatgaaagg tttttggtgg aaaaacactt
    13321 caaaaagccg gttattctca ccgattaccc aaaagaaata aaagcctttt acatgaagca
    13381 gaacgacgat gggaaaaccg taagagccat ggatgtactg tttcctcaga taggagaaat
    13441 tattgggggt tcacagcgcg aagaaaacta cgttaagctt ttgaacagaa tgcacgaaat
    13501 gcatataccc gaaaaagaaa tgtggtggta tctcgaaaca cgaaaatttg ggtctgttcc
    13561 tcacagcggc tatggtctgg gtttcgagag gctcatgctt tttgttacgg gtatgagcaa
    13621 tatcagagat gtaattgctt ttccgcgtac accgaaaaat gcagaattct aactttttgg
    13681 taaaaatttc gtatttttaa caaaaaacag actatgctta agcaaagttt acaacaaaag
    13741 ctgttacaga aattgtcgcc tcagcagatt caaatgatta agctgctgga attgcctaca
    13801 ctgcaacttg aacaacggat aaaaaaggag ctggaggaaa accctgctct ggaggaaggt
    13861 gccgatgagg atgaaaacac cgataccaat gaggacgaaa acgaagagca aaacgaaaac
    13921 gatgatgaat tttcgctcga agattatatc gatgacgagg atgatattcc tgcctacagg
    13981 ctgcaggtga ataacttttc gaaagatgac gagcgcaagg aaattccttt ttctgccgga
    14041 atcagttttc acgagaacct gaaagatcag ttgggactta agcaaatcag tccgcgcgac
    14101 agggttcttg ccgagttttt aattggaagc atggacgaag acgggtacat acaccgcgac
    14161 cttgaatcca ttgccgatga tctggctttt aatcagaata tttctgctac cacccaggaa
    14221 atcgaaaatg cgctttatat tgttcaggac ctcgaccctc ccggtatagg agcacgaaat
    14281 ctgagagagt gtcttctcat tcagcttgac cggaaagaga aaaagaccct gccggtgctt
    14341 aacgcacacg aaataatatt ctcgtatttc gaagaattta ccaagaagca ctacgacaga
    14401 ataatgcaac gtcttgaatt gagcgaacct gaactgaaac aagcactgga aatgatactt
    14461 cggttgaacc cgaaaccggg aagctcttac agcgattctt cggtaaggtc agcgcagacc
    14521 attgtccctg attttatcct ggaaaactat tcagggaagc ttgagttgag cctcaatgca
    14581 cgcaatgcac ctgaactgcg cctcagccgc gactattccg aaatgcttca gggcttatcg
    14641 aaaagcaagg attcgggtag ccggtctgag aaagatgctg ttgtttttat caaacaaaaa
    14701 ctcgattcgg caaaatggtt catcgatgcc atcaagcaac ggcaaaacac cctgctcatt
    14761 accatgaatg caattcttga atatcagaaa gattttttca tacagggtga tgaaacattg
    14821 ctgaagccta tgattttgaa agatatagcc gaaaaaaccg gtctcgatat ttccacgata
    14881 tcaagggtgg ccaacagtaa atatattcag actccctttg gtaattattt actcaagcat
    14941 ttcttttcgg aggctatgca aaccgattcg ggtgaagaag ttagcacccg cgaaataaaa
    15001 cagatactca aggaatgtat tgagaatgag aacaagcgcc gacccctcac cgacgacaaa
    15061 ctggcttcaa ttcttaaaga aaaaaaatat gttattgcca gaagaacagt tgccaaatac
    15121 agggagcagc tgggcattcc cgtggcacgc cttagaaaaa agctctgatg gaaactggta
    15181 aattcctaaa cactattgca aaaattatca gtgttttgtt tcaccccttg ataattccaa
    15241 cttttggctg catcgttatt ttcaacagtg gatattacgt cggattactg aatccgaaaa
    15301 ttatgcaggc ggtatatatt gttgtgttcg tgcttacttt tttactacct gcattaataa
    15361 ttccggcact ttatttacag aaatacatca cctcatttgc tattgatgaa aaaaatgaaa
    15421 gattgctacc cattacaata gttgcagtga tgtacgggct aagttattat tttatgcagc
    15481 gaagtgggtt cccaccgatt ttacttcatt ttattctggg gtgtattatc tgcttggttc
    15541 ttggtttgct gattaccata aaatggaaaa tcagcttaca catgattggc ctgggtggcc
    15601 ttaccggcct gattgttttt cttgcgcaca gttacggcct tggcttgcat tatttcctga
    15661 tctttgtaat acttgcctcc ggagtaacag caactgcccg cctgattctg caaagccata
    15721 accaattgca ggtgtatgca ggctattttc ttggcgttgc cggcatttgg ctgagcatga
    15781 tattcttata aatacataaa agattggcag aagagacacg taaacaatag tcaggatttt
    15841 tttctacttt tgcccaaaat accgcaatgc ctttcattgt aaaattcata ctgtttttat
    15901 ttctggtgta tatcattggg aaaatactcc tccggtatgt gtttccgtac ttaattaaat
    15961 ggtggctgag aagaattgca aagaaaatga atccagaagc ttttgaaaaa ccctccggca
    16021 aaaatcggaa agaaggcgat attaccatta aaaacatgcc caatacgaaa aaaaataagg
    16081 gaaagtccga cgaaaaaggc gaatatatcg attatgagga attatgaaat cgagcatgat
    16141 agtaaaaaca ccaacagtga tttatatcga tttatgcgag attctatctg acctattgta
    16201 taaattataa acagatgaaa ttcatacgaa aattactata caagtttctt cgaattgaaa
    16261 actatctaag gcttgttagc agtgtgtact tattgctaat aaaggccggt ttttataaac
    16321 gcaaatatcc cgagatattc tttttgaagc aaattattaa agagggtgat gtttgcctgg
    16381 atattggagc taatcttggg tactattcat ggttcatttc gaagttctgt ggaaaggacg
    16441 ggaagctcat tgccgtagaa cccgtacccc tattcgggaa aatatggctg cgaaatatga
    16501 aaagctccaa gtttgcgaat ttcaggcttg aatcctgcgc tttagggagt gaaaataccc
    16561 gtgtgaagat ggctaccccc gaggtaaacg gagttttaca ccatggcatg acccatgtgg
    16621 ttaatgaagg ggaagcccaa caatcaacct gttacgaagt agagatgaaa attccggatg
    16681 cactgttttc ggcacttgat cgcctaaact ttgtgaaaat cgatgtggaa ggttacgagt
    16741 ttattgtgtt ttcgaatatg gtgaatacaa tttcgaagtt tcacccggta attcaagccg
    16801 aactcagcgg cagcgaaaat cgattgaaag tgttggcatt gctcgaatcg ttgtattaca
    16861 aaccgcatgt actcgaaaac cagcaactgc gggctgttaa tcaatcagac attgaaacag
    16921 gaatcagtga tttttacttt atttaggatt ttagaatttc gattatattt ataataaata
    16981 ctaacacagc aaatagcggc agtcgatgaa aaaacaactg atattattca ttgcacttct
    17041 agcagtaact tgcatacttt atattcctgc attaaaaaac gaatttaacg attgggacga
    17101 tgcagcttat gttacatcaa acccaaacat tgaactcaca aaagaaaata ttgtccgatc
    17161 atttacacaa ggcgaacacc accgcatgta tgctcctatt actgctttgt cgcattcagt
    17221 agtttacact ctttttgggc tgaatcctaa accctatatt ttgggtaata tccttattca
    17281 cctatgcaat actttgcttt tgttcttatt tctttcgcag ctcattaaaa aaccattggt
    17341 cccgcttttt gcagcagctc tgtttgctct ccacccaatg caggttgaat cagtggcata
    17401 tgtttccggc aggagagatg tgctgtatgt ttttttctat ctgataagcc tgatatacta
    17461 cgtcaagtat gccacacaag ggaaaaacta tattgtaaag tatttgctat cactgatatt
    17521 tggagtactg gctttgctgt cgaaaggaca agctcttact ttgccattca gcttgatttt
    17581 gatttcgttg tttaccggcg taaaatggaa ttcgaaaaag ttctggctcg ataaaatacc
    17641 atttttaatc ctgacagcta tttttgctgt gaaggtgttt tctgcccccc agtttgcatc
    17701 aggaaactat ttaagcacag cctacctgga ttccgacatt ccattttttt atagcattat
    17761 ttatgcttgt tacggatttg ttcaatacat catattgcta ttctttccat accgtctttc
    17821 attgatccat ccatatcctg aagttgctgg tgcatatgta ataccttatt acatgtatat
    17881 ttatattgcc attcttgcag gactgattat tgctttcttt cgatttgctg tcaagcgaaa
    17941 gcttttttgg ttcggattta ctttttttgc aataaatatc gccatgcttt tgcaaattat
    18001 tccgaacagt tacggaataa tgaacgacca ctatgtttac ttttcgggaa taggcatctt
    18061 ccttataatg accgataaaa tcaccgaagt gatgaaccat agaaaatgga gtacattgat
    18121 tgctatatta ttttcggtgt atttagtcgt tctatgtgga ctaagttttc agcgcattgg
    18181 agtttttaaa gattcaataa aactatggag cgatgtaatt aaaaaatacc ccgaccatta
    18241 catggcttat agcagcagag cgaatgccaa ttttaatcag ggctttttcg agaacgctat
    18301 tatcgattat aagaggtcta tcgaattgaa tccggagaat tacgctttgt atgttaacct
    18361 tggatcagcg tatagtgcag cacaacaata caaaaatgca gttatcgctt tcacaaagtc
    18421 attaaatcat aagccaactt ctgaggcgta tactttcagg gcagacgcat attcggcact
    18481 aggtaataca aacgatgcaa tcagggatta cagcaatgct attgagttga agcctgatta
    18541 tgctgtagca tacaataata ggggtgttgt ttacgaaaaa atggctgaaa ccgaaaaagc
    18601 aatgcaggat tacaaaaaag ccttcgatct cgacagtaca aatattgatg catgttacaa
    18661 ttgtgcaaaa ctttatggga acaacgggaa cttcgaaaag gctttgtact accttgataa
    18721 gctcattgtt attaaacctg attttgcagc agcatataat tcaaaaggta ttatttttgc
    18781 cactaccggt tcttatgaag cggctattct ttgcttttca tcggctatcg aaaacaagcc
    18841 tgattatgcg gaagcataca ataaccgtgg atcggtgtat tatcgcaccg ggaagtttga
    18901 cctggccatt gctgattata caagagctgc agaaatattt cccggttttg ctgagacatt
    18961 caacaaccgt ggggctgcat acattgcttt ggacaagaag gatagcgcct gttccgactt
    19021 caccattgct gcccagcttg gtttggacag cgcaaaagaa aatttggata agttctgcaa
    19081 ttagaattgc gaatttttag agacacttcg atagttctat tgcaggtagt ttcttgccaa
    19141 ccgtacaatt cttatgcttc aggaatatac aggaatgact tcaatgattt tatcgtacac
    19201 agtttcgacc gatatcgatt gcatacaggt attgctcttg cagtgtggca accgatggtt
    19261 gtatggattc acgcaggggc ggcaacttaa atccgattca ataatatgcg tgtttttacc
    19321 gggaggcccg aataaaaccg gtgtttccgg accaaaaagc acaatattat gaatacttgt
    19381 aagcgatgcg aactgtcccg gtccactgtc gttggtaacc aatacatcag caagcgaata
    19441 caatgccagc aattgccgaa catttgtttt cccggcgaga ttgattactc cgggaaaatt
    19501 tattttgtcg cataattcgt tgcaataagt tgcttcttcc aacgaaccgg taattacaat
    19561 ggttaattta cctttataat gtgatgagag tttgtttgat aaatcgacaa agttcgatac
    19621 atcccatttc cgtgcaggaa tcgaatcgcc tgcattgggg tttattataa ttatagggga
    19681 tatgttttcc tcttgcgaaa cactgtgtat gattttcttt atttctgcta cgtcgtctct
    19741 cgtaatttca attataggct gcaaaatttg gattgtattg atatcaattc tggggaaagg
    19801 cctgatttta tgattacata gcaatgattc aaccattaac ctatatgcaa gtgctgcgtg
    19861 atgatagggg ttgtattgca atttatgtgt cattaggtcg ccacgataaa ttacttcgct
    19921 atgataacgc tggtagccaa ctctggtttt tgctccgcag caataggcaa gtattgctga
    19981 agatcgagaa aaaaactcaa gatcaatagc ggtatcaatt ttctcttttt taattgccgc
    20041 caaagtctta agtatttcaa aagaagtcgt aataaatcct ttgttatgaa ttgtaaaaac
    20101 attcttttca tgaaaaacat taagtatttc gagtatcgga gtgttttcgg caaatgccca
    20161 aaaataaata ttttctttac ccaccatatc ttcggcttct tttaaagcag agtaggctaa
    20221 aacagttgaa ccttgctcgg tgagtttgat gaaaataatt ttacggtgag tttttatatc
    20281 tcttctacgt gatacgaata ataaaatcaa atacctccac agtgttagaa gaaaaaccaa
    20341 aggaattcct acccaaaaat cgagatgttt tatttgacgt ggtttcattg gtaatttgtt
    20401 gaacaaaaat aatcaatata agcaacaatt acgatttcaa attacacgag aaaagcaact
    20461 tatcgaaaat ggaaaatttt gcaaacagtc aacaaatcac taaattatta atatttcgtt
    20521 tacttcaata tgacttttct gccggcgtac tgttgcagga caattccgga tacaatgaga
    20581 acaagtccga taatggttga ttccataatt ttttcgccaa ccacaaaata aattccgatt
    20641 aatgataaaa atggtgaggc aaaaataaaa ttgctcactt tagcagttgt tgaagaatat
    20701 ttcagtgctt tcgaccaaag caagaaagtt attcccattt cgaataagcc tgaatataca
    20761 acagccagtg ccgaaaccgt aaagttaaac gatggtttcg aaaatataat acatgcaatc
    20821 atactgaata caaaaccgaa agcaaagctc aaaaacaatt taatggtttc gtcgcgtttg
    20881 tctttcatat tgaaaatcca gaaaaacgcc cagattagtg agctgccaag tgccaatgct
    20941 gcgccggttc catttgtaaa tttcatttcg agaagattac catgcgtcga aattaccagc
    21001 accccaacga agctgattat gatggcaagc aagctcatcc aacttatttt ctgctttagc
    21061 ataggaatag atataagcac cagcatgatg ggccatacat aattcaaggg ttgggcttcc
    21121 tgagcaggta aaatgtcgta agctttaaac aaaacaaggt agtatgcaaa cggattaaga
    21181 aaccccagca ttgcagactt tatcaaatcg cgaccggttg tgcgcatgag ttctctccat
    21241 ttacccccaa taacaataat gacaagcaac accagtgcgg ctgtcaatga gctgaaacac
    21301 acgaaattga tgaaatcgaa ttgtttgagt gcaattttaa aggctgtggc taccgttgac
    21361 caaagcagaa cggtgagtac cgcgtaaatg tatgctttgc tctggttttt caagattatt
    21421 tcgatagaag tttacgcaaa gttgcatcat cgaccctgta aacgggtaca atatctttct
    21481 tcctgtccca gtaaacagtc ttttgataaa accagttcag aatagctctg gggttggagg
    21541 caaactcctt gtcattcatc attttatcgt taaactcagt gtttagaaaa gggtcacttt
    21601 tcagcatgct tataaccatt gcttccataa catacgattc ggcatattcc ttttgttcga
    21661 ataccgcatt gaaaaatccc cagcgcacca ggctggccgg tgaaacaggt tcgaacatat
    21721 atgccaataa gcgtgcataa ggattgcttg tctcaacaat gatgctgcct gccggaaaca
    21781 ccaaactgtc gtaagttact tctgtggcaa aatctattat gcgttgcttc ccttcgtaag
    21841 gctctgaagc gaatttcaca ttcttgagtc ggtatgcttc caccggtact ttcatctctt
    21901 tgtacagacg gatatatttt acaccctgag catcaagaac agcagtaaca tttccccata
    21961 ccggttcgaa aacgtaggct gccgggagtg ttgtctgtat cgaaggtctc ggattgttga
    22021 agaaccaaac cttgtgaatg gcgggagtat cggaatattg aaaccagttg ccaccggtaa
    22081 tgagactttt tacgacggtg tattccacac cttcgaaatc gatttgtaca ctatcggcaa
    22141 ggctgttgaa atcaacactt acgggttttt tgttcttgta aagtttcgaa acggcaaggt
    22201 ctgattcagc attgagttta atgataattt cgtgttgagt atccaccagc gaagcagtgt
    22261 atttgagtac ttcgtaagta gcatcaacgc gcttcccgta aggtttaagc atgtgtgttt
    22321 cgagcagaag ccccggcctg ttacgggcag cagcatatcc ttcggaaagc acaggcgacg
    22381 gaatccacct taccagtccg cttttggggt catgccagtt cttaaatgtt acatagcgaa
    22441 acatagggaa gtctttttcg cgcatacgtt tgagcagcgg ttgcatatat actttatcct
    22501 gccactcgtt ttgtgcatcg ctaagcaaac acatatcggt caagtcgtaa gtcatcacat
    22561 actgataatc ggccccgtcg gtagtatggc agtcgataaa catatcgggc ttccatgtgt
    22621 tgaaaacctc aagaaaacat cgtatttcgg gtgtttcggc ttttacgtaa tccctgttga
    22681 ggttcttgtt tgctgcattc gttcgccatc ccatttcttt gggaccgttt tggttaatgc
    22741 ggttataagc actgcgacgc aagcaacctt ctgggttaat tgttgggaaa aaaagaataa
    22801 tcaggttttc gggaggccga atttttcggc aggcaatgtc tctgaaaagc atcattccgg
    22861 catcggtacc atcaggttca cccgcgtgaa tgccggcttg aataagtaaa actgattttc
    22921 ctgcttttcg tgctgcaatg ggatcaaatt ttgctgaagc atcatagatt aaaagcggaa
    22981 tatcgtagcc gtttccagtt ttgcctatcg aaatgtattt aatgtaattg caggtgctat
    23041 caaaaagctt gctgtaatgc aaaatttcgt cgtaggtcat cgttgctgtt ttctctgagt
    23101 gctcgtaggg ggtaacgatc agcttttgcc cgaatgctat gggtaatcca acaacaataa
    23161 aaatagtaaa taaaaatatt ttgcgactca taaatcaagt ttattagatg caaagatagg
    23221 aatgtttttt acctaaactc ttgtagctgt aattgtaata aagtggcgag taagtttgaa
    23281 aaaaacaaaa ataaatttgt aaaataaatt tgtaaaataa atttgtaaaa taaatttgta
    23341 aaataaattt gtaaaataat ttacgcatga aaatacttaa aatacgacat ttaatattag
    23401 ttgttgctat tacaataata gcaatattag catgctcaaa aaacgaaccc atttcacagg
    23461 gaacaagtat taatttgcga tacaacggag acgatgtgcc gatgaatttt gcgtggtatc
    23521 aatgccaatg taccactcaa aatggaactg tatattcggg tcaaagatgc gaagtaacaa
    23581 taatgatccc caattgtact cgtcaggatg ctgagttagg atgcatctgc aagacacgcg
    23641 ataaagttat atacgataaa acgtcaaaac taacacgaaa tgcggatctg actattagcg
    23701 aattagattc tgtaattgaa attttaaaca gtgaacctat tacaaaacaa gtaatatcga
    23761 atcattatcg tgtattttat tttctttata cagaaggcat tcttgagcac cctgatactt
    23821 taattaaata ttcaattcta taaaactata acaatgcgat taatcaatca gtatattacg
    23881 gttatcagct ttatagctcc gcttgtaatt tataccatga tattttgggg ttgccatcat
    23941 gaagaaacga tgaaattcag ttttcatgtc ggaaccgact cgctgatttt gaaaaatttt
    24001 acaggaggcg taggatctgt gaagtttctt gagcagggcg atagtgtgtt tgtatttatg
    24061 ttgaactcag ctggagataa attttgcagg gtaagctata ctccgtatct tactacagat
    24121 ataagtgttg tttcaattcc aacgcaatta cgtgaaaaca aagttgcagg cattcaacag
    24181 gcccgatcga ttttacctat tagcaccgac agtattatgt ttgcgcagaa aaccgacgat
    24241 tacggattta ttttttcttt atatgtgatg agtaccggta caattcagcc gatttacatc
    24301 tcaaaaccgg aaaatagaat attttttcaa gaattgcccg aagatggtat caattggaaa
    24361 tacgataaaa agttgcgaaa aattattgta gccatagtaa ggcaagatga ttacgacgta
    24421 agaccatacc cgatcggaac ggaagcattg gctggcattg atatcgcaac gggagaattc
    24481 gaaattctac cgttaaaata ccctgttaat tatgaagaaa aataccagag caatatttcc
    24541 cggagcgagt atttttgttt gaacgatagc ttggtagtat taacttttgc aatgcacgac
    24601 actgcattta tttataatac tacaaccggc atttgcagta aaaaatgtat ggcttcaaaa
    24661 agtgacaggc aatttctaac aattagtcag gatgattatg aaaattgtga aaccgaaaag
    24721 ctacgcgatg caaatttgcg gagttttaaa taccgttcgg ttttttatga cccgtataga
    24781 aagatgtatt ttcgttttta cgagcagttt atgcctgcga aaaacaacga tgggctattc
    24841 aatatttgga gcgataaaca aattggattt gcctgttttg atcaggaatt taactatatt
    24901 ggagaagact gttttggaaa aatgggtgat gctgatgtaa tgtttattgc aacagggcaa
    24961 cccgtgcgtc ggggcatgct gttgttttta aattataacc aaactcaagg cagtgttacc
    25021 gcaaacaatt tactacgatt aatgcttatt caagttgaaa ccaactgacg caatcaaatg
    25081 gattttatta tgtttctgtt tcatggcttt gtcgtgtcac gacaaaccac attatgaaag
    25141 agaaaaagaa ttaatttcct atctcaaaac caagcatccc gaagtgttgc aacaagatcg
    25201 gtgtgggctt ttggtatatc gtaaaccata tagatgtgat ttatttttcg atgcagggct
    25261 ggtaaacgat atcaacaatg tgctttattc gcagaaaccc gaaaagcttt atgttattta
    25321 tgatttcgaa actacgaaag attctttaga aacgaatatc aacttcgaga acaaagttta
    25381 ctttagggag gattataatt gtctcgacag atatggtttt cggttagctc ccatgttttt
    25441 tattattaag aatggtcgga ttgattattg gcataccatt accaaaggat gcgattagca
    25501 ttcaatgttt tccagttttc acacaatcat tttaatttgg taaaagcaca tcagtgccct
    25561 tcaatcgaat ggctttatac atcatggcaa agaaattcaa taagttcttc ttccgacagg
    25621 ctgaataagg caacaaaccg ttcttcgcta cacgcaataa gtgaagcaat tatgtttttt
    25681 gaaagaactt gagcagatgc tttctggtag gcagtggtga atttattcaa ctgcatgaga
    25741 tagtccgaat tagtttcaac atcacctgaa ataccataat aagtggcaaa atgttgtcgg
    25801 agtttattca gcaataacgc aacttgttct gtgttggtta tttcgggtat ggcttgtgaa
    25861 ggaattgtta catctggttc cttctcgtga gcccataatt cgagttgcct gatcatggca
    25921 tcatagctcg cacggttttc gcggtcgcga atgggactga acttgggcat gtgcttttgc
    25981 atataggtca tgacctcaag aattttattc ttttccatgg tttttaccga aagataggcc
    26041 ggatgctcta catagttcat gaaaatgttg aaacggttct ttatggcaaa cctgtagata
    26101 tcgggtactc cggaataatt attggtcatc aggcaggtac aggaattaaa attggtcttt
    26161 ttcgatttgc agtacccgat aaagaaatcg agatttcgac gaaaaacatc gaaagaagca
    26221 tttcttcgta tttcttcgta tttgtccttt tccagcgaat cgatagaaat atttatatcg
    26281 aagcgtccgc gctcaagtat atcacggact tttttgttta ataccgttcc gtttgatgtt
    26341 accgaaatta tagtttttgc accggaagca atgatttttt cccagatttc aaaataaagg
    26401 tctattaaaa aaggctcacc tcctttaaaa ctggctcttt tgaggtaagc taggtaaggc
    26461 gtaagttgtt caacgaaatc agtgttgtaa gggctgggta aatcaggctt tttttctgaa
    26521 tgtatctgat cagaaagaac agggctgcac atgatgcaag ccagattgca gtgattgctg
    26581 agttcgaatt cagcagtaag tggaatacca gattttcggt atgcctggtt atagacctga
    26641 aaagtagcag cttttggatt gttgctttta agtttttcgt aacaacttct gcaatcagtt
    26701 gggatgtctc ctgataaaaa agattctgtt gcctgtcgtc gaaattcgcc ttccacaatt
    26761 tcactgatgc ggttctcggg gaatttgcct aaaacaagtg agtgattatg gctgcacata
    26821 cttacatcgc cattgatgtg aaaatacaca tttgaccgaa gcgcaaagca atgatgctta
    26881 agctttctgc cccagggtgt gagcctcctg aaccggtgat aatagctttg aatattgtat
    26941 ttatccatga attgaagtgg ctcgcgatcg gtgttttaac atagcagggt tatcaaataa
    27001 atccagtatt caaaattaca attaaaatgt ataatccgaa tgcttatctt gatttgctta
    27061 aatatcggaa ttgataattt tcttaatttt agaactttta aatgacctac ccatttaaat
    27121 gttggacttc gattattgtc ttaaagagaa taaactgaaa ctatgaacta taagaatagc
    27181 aaaattgtga gcatacttac tagctttgga ttctggggca tttttcttat agtggctctg
    27241 gctgttcaga tgatattgtt catgctattt tcgtaccagt caatttgcat agcaagtggt
    27301 gtgttattct gggctatccc atggtggcta acaacgataa tcatgaggca taacagtcag
    27361 aagttgaaaa cagcaattca cagtgttttt ggcacattga tgttggttga agtagtttta
    27421 agggcttcgg gtttggtggc aaattattgg gaaaggcaac atggctatta taagtcggtt
    27481 tacgacgttg attcttctgt ttttgttcaa aagaggaccc caggggttta tcagataagc
    27541 aacagagatt gcagcttcga aattacaacg aactcatggg gttaccgcga taaagagtgg
    27601 acccctgaga atatgaaatc gaaaatccgt atcatggcgt tgggagattc atttaccgaa
    27661 ggttatggag ctcccgctga tagtacctgg atgaatttac ttgaaaaaga aattgccgat
    27721 accggctatt attttattaa cggcgggatg atagggagtg accctgttgc tgattataac
    27781 cggttgaaaa ccaatcttat agaattagac cctgacataa tcctatttgc tattaacata
    27841 accgacctga gtgatttgat gttgcgaggc ggtcccgaaa gactctcaaa ttcctcagtt
    27901 gagaaactga aggctccatg gtgggagctt atatatgcaa tgagtcatac actacgtctg
    27961 ggaataaacc agttctatga agccagcctg ttaatttcgc ataaacaggt tgccgttaaa
    28021 caggaaaaat atttgttaat aatggaagat ttttgtaaaa atgtaaccaa tgagttcaac
    28081 gacagtatta
//
LOCUS       DAOOQP010000025        27497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2055_length_27497_cov_5.463560, whole genome shotgun sequence.
ACCESSION   DAOOQP010000025 DAOOQP010000000
VERSION     DAOOQP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_27497_cov_5.463560"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLK75_04395"
     CDS             complement(<1..485)
                     /locus_tag="PLK75_04395"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOY38518.1"
                     /translation="MRSDIEIAKAADIKPVIEIAASLGIAEGQLELYGKYKAKLPLSL
                     IDESKMLNSRLILVTAITPTPAGEGKTTTSIGLAQAMNLLGHKTTVVLREPSLGPVFG
                     VKGGAAGGGYSQVIPMEDINLHFTGDLSAVEKAHNLLAALIDNQLQNRSHDFDLDART
                     I"
     gene            complement(574..1746)
                     /locus_tag="PLK75_04400"
     CDS             complement(574..1746)
                     /locus_tag="PLK75_04400"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOY38519.1"
                     /translation="MLRVILIFLSLGITSSFSSAQTFSCTQVYYPKEDAVVWYLTNQN
                     NPQWGPTNTQNYSTANFLDTQVWTWNGSPGKKRAYLANDFENLIDLTSTYIYSCHLNL
                     FNPFSNTAFAHRYGEGQINTTRIYKVPEYWVSTAITWNNQPDIETDEYAEISPIPDSP
                     NTDSYEDILNIDITNVVLTDDHTLESNFHGLVLMQWDEDLDSYFHKMNFASRDYNDPA
                     YWPQICITYVFPEPIVYFDGSSLTVTGVEDLESLFYDHVSYYWLIDNESFEGKTIPFE
                     TDNIDENSLELHIKITNSIDSLCEYVFTPVVSAIHNNTLLAIQIYPNPVKENFIVVNC
                     AEGFGITILNASGTLVFSGDNFYSRQPIDISGLKSGVYIVRIITGDDTVNRKLVVE"
     gene            1844..2548
                     /locus_tag="PLK75_04405"
     CDS             1844..2548
                     /locus_tag="PLK75_04405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY38520.1"
                     /translation="MKLRPLFKGLFTVCRGHYIVPGNCCVFVGQMSRLSKWIHKHKRI
                     AGYSDFYDIHFRYNKREDLFRYLIENENLDAVDYLEFGVANGCSFKWWTENNTNTDSR
                     FIGFDTFTGLPEDWGPFKKGDMSAGNAMPEITGNRHQFVQGLFQQTLPGFLKSFDFKN
                     RKVIHMDADLYSATLFVLTSIAPYLNKGDIIMFDEFNVPMHEYKAFTEFVSSYYIEYE
                     ILGAVNNFYQIAIKIK"
     gene            complement(2553..3980)
                     /gene="rodA"
                     /locus_tag="PLK75_04410"
     CDS             complement(2553..3980)
                     /gene="rodA"
                     /locus_tag="PLK75_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOY38521.1"
                     /translation="MQTRRGKIWLNIDWICIALYLLLVFAGWVNIYAAVYDESHTSIF
                     DTSMRYGKQLIWIAAAFIIGFFVFLIDSKFYSAFSYVIFVFFMLLLIAVLLFGTEINN
                     SRSWFQIGDFRLQPSEFAKMATSLALAQFASRTHFSLNNLKDVLGTILIIGLPIGLIV
                     LQNDAGSALIYSSLIFVLYREGLNNTLFVLFFAAIALFVFALVSNQLIIMTVLVLAAI
                     LYFIFTSRKRWYGYWGLLVFAVFAAAAILVQYFGIWPYPLKYLILSAVVLSIGVSLLI
                     ALLKKIDKAASVLVILLAASLYVYSVDFVYSNLLEKHQQERIGELLGFNSDPLGAGYN
                     VNQSKISIGSGGLLGKGFLQGTQTKYNFVPEQDTDFIFCTVGEEWGFAGSMIVLMLFA
                     FLIIRIIWLAERQRSAYSRIYGYSVASFLFIHVAINIGMTIGLAPVIGIPLPFFSYGG
                     SSLWAFTMLLMIFLRIDANRMEVFR"
     gene            complement(3961..5784)
                     /locus_tag="PLK75_04415"
     CDS             complement(3961..5784)
                     /locus_tag="PLK75_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOY38522.1"
                     /translation="MNQLSSRSIYITVIILLIFLAILVKLFIIQVLDPSYKISSENNS
                     QRRIWEYPSRGLIYDRNGELLVCNEIAYDLMVIPRQVRSFDTLQLVNELGINIEDARQ
                     KLTAAAAYSVFRPSVFFKQMSKLQYAYFQEHMYRYPGFYVQSRSLRKYEKSFASNVLG
                     DIGEIDMPTLERDTFYYIGDYVGKSGVEKYFETSLRGEKGVKIYMVDVHNRIQGSYQQ
                     GKYDIPAQAGKDLVLTIDAGLQEYGELLMKNKIGSIVAIQPSTGEILAMVSSPGFDPN
                     LLVGRQRGYNYDSLVHAPNKPLFNRAVGASYPPGSIFKMVQAIIGLDQGIITEQTSFP
                     CDKSIVNCHNHPPNTDLKRAIQYSCNPYFYMVTRKLIQRGFEKSIFRDSRIGLAIWKE
                     KVLNLGFECAFDIGLPSVVKGKIPGPDFYDRIYGKYRWAYSTIYSLSIGQGEVLVVPL
                     QMANFAAIIANRGFYYYPHIMKSIGGAPLDTMYTIPIRTPFKPEVFIPVVEGMDAVVN
                     EDYGTGGLARIPNIRVCGKTGTAQNPHGEDHSIFMAFAPKENPQIAIAVYIENAGFGG
                     VWAAPIASLMIEKYINDTVMNVFSEQRILNTDIYHFADTAR"
     gene            complement(5781..6290)
                     /locus_tag="PLK75_04420"
     CDS             complement(5781..6290)
                     /locus_tag="PLK75_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOY38523.1"
                     /translation="MINSNLKYFFLFIICIALQVLLFNQIQSIPYTHVFFYILFILLL
                     PIETPPFLLMLLAMATGLVVDSFSNTFGIHAAASVLTAYIRPGMLRLYSPRDGYETNT
                     HIGIRDLGFLWFLKYAASLVIIHHFLLFMLDAFSFKMFQFTLLRITLSSISTISVLIL
                     SQYLTIRRR"
     gene            complement(6277..7095)
                     /gene="mreC"
                     /locus_tag="PLK75_04425"
     CDS             complement(6277..7095)
                     /gene="mreC"
                     /locus_tag="PLK75_04425"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOY38524.1"
                     /translation="MKSLFRFILRYHATFLFIILELICIALLVANNQYQRSVFASSSY
                     VVISRIEKSWSVVTDYVKLAGENRRLAIENAALQNELQQRRAYNRSFVFDSVNPDYTF
                     MNVTVINNSVNNVKNYITIDAGLKQGVRKEMGVIGPEGVAGIVYNCSDNFSTVLPVIN
                     TAFKLSVKISRNNYFGSLSWTGKSHRYARMSEIPVYIDIKVGDTVVTSGYSSIFPPDI
                     PVGVVDSYKKMTSTGFYEIDVKLATNFANLKNVYVVKNLHKQEIEALEAQNDKQ"
     gene            complement(7109..8131)
                     /locus_tag="PLK75_04430"
     CDS             complement(7109..8131)
                     /locus_tag="PLK75_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOY38525.1"
                     /translation="MGLFSFLQQEIAIDLGTANTIIIHNDKIVVDEPSIVAIDERTGK
                     LIAIGKKAREMHGKTHQGIRTVRPLRDGVIADFNAAELMIRGMIKMLNKKRPLFTPSL
                     KMVVCIPSGSTEVEIRAVRDSSEHAGAREVYMIYEPMAAAIGIGMDVEAPGGIMVCDI
                     GGGTTEIAVISLGGIVCNRSIRIAGDEFTDDIQEYMRHQHNIRIGDRTAEEIKINVGA
                     ALPNIDNPPPDYIVRGPHQMTALPIEIPVSYQEIAHSLDKSISKVETAIMMVLEQTPP
                     ELYADIYMRGIYLAGGGALLRGLDRRLSEKTNIPVHVSEDPLHAVARGTGIALKNVDK
                     FQFLIR"
     gene            complement(8402..9229)
                     /locus_tag="PLK75_04435"
     CDS             complement(8402..9229)
                     /locus_tag="PLK75_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY38526.1"
                     /translation="MSNYKILVIDDEPVNITAIKTILSNEPIEILAAYNGWSGIEIAT
                     AEKPDLIILDCQMPVNDGYTTLVKLKKNPVTSPIMVVMITGVMKSYYYVLNTLNAGAV
                     DFIRKPFDETELRARVRSLLKLVDYHKRELAQKDVELVQSAIKIVKCNEFNINLIQML
                     NQMIGEGDVQTVPVSRLIEMREVIENKLHTDSWKQLEDHFNSVNPDFQNALVSKHPNL
                     TPSEIKLATLLRLNLSTKHIAGILYLSADSVRVARTRLRRKLNLKPEANLTAYIFSL"
     gene            9410..10402
                     /locus_tag="PLK75_04440"
     CDS             9410..10402
                     /locus_tag="PLK75_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38527.1"
                     /translation="MNSEEKPHNSQNEVNLLQRSSDKMLDAFESMLFHELMNHLTFVV
                     YPPDSLPAEESLEFSKFSGKQILTMLNTIRYVKLYEKNELRALPSAIHPGQLFEKTIS
                     EFKPITSGKNQTCTFEHDGIDKIIADEELLKIYFWNILSIFTKYAPEKSIFEVKTTAS
                     ENFLTLDISHPHSYAGEYYKAVNFDNFGDALLNNQGFERSIAIKLLACKYMMAIHNAA
                     FRIFSIGDRTNFSARFSGFDADKRENDNNDEKNKNIRLDKAGMKKTEPLIQALKNTDF
                     YKITRIYDILSEIEMADPCENTQAWAKTAKMVLESGKKDDFEAFLDNTLNYFHS"
     gene            complement(10393..10863)
                     /gene="rlmH"
                     /locus_tag="PLK75_04445"
     CDS             complement(10393..10863)
                     /gene="rlmH"
                     /locus_tag="PLK75_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOY38528.1"
                     /translation="MKLTLILTGKTDAGYLTEGTELYFKRISRYIDFKLIIIPEIKSK
                     KTNVTELKSAEGVQILKNIGSDDYVVLLDENGIELRSVEFAGYLNKKMVAGCRNLCFI
                     IGGPYGFSKNVYAAANDKISLSRMTFSHQLIRLIFAEQLYRAFTILSNEPYHHE"
     gene            10962..11363
                     /locus_tag="PLK75_04450"
     CDS             10962..11363
                     /locus_tag="PLK75_04450"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOY38529.1"
                     /translation="MKIIRASFLFCSLFFLTSQASQAFPILDIPLKIFQAMKVGNVDE
                     LSKYFNTSLELVVLDKEDVYSKQQAVQILKDFFTKYKAKNFTLLHQGGKEEAQYAIGT
                     LETEKGNFRVYFLVRQKGAQTLIHQLRIEEE"
     gene            11360..12205
                     /gene="nadC"
                     /locus_tag="PLK75_04455"
     CDS             11360..12205
                     /gene="nadC"
                     /locus_tag="PLK75_04455"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOY38530.1"
                     /translation="MIGKTYLLQLIKRAFTEDLGPGDHTSEACIPSNAFGKVNLLVKE
                     PGIIAGVNIAKEVFRFANPAIRVNTFIEDGSQVNTGDIVFTAEGSQKSLLTAERTVLN
                     FMQRMSGIATETRRYADAVKPFNTQILDTRKTTPGLRLIEKEAVKIGGGTNHRIGLYD
                     MIMIKDNHVDFSGGIVQAIENVHNYLRAHSLYLKIEIEVRNFVELGEVLNHGGVDRIM
                     LDNFSIENTATAVEQIAGRYETESSGGITLQNIAGYAACGVDFISVGALTHHIKSLDL
                     SLKAL"
     gene            12205..13143
                     /locus_tag="PLK75_04460"
     CDS             12205..13143
                     /locus_tag="PLK75_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOY38531.1"
                     /translation="MKIPYQNKLLRKLQKPISWLKRRSFPGFDGYPIYDVGAFFVSGL
                     MKGHLGLRAAGVAFNIFLSIFPLIIFFFTLIPYIPIENFQTELLQFINDVLPNEANTL
                     FNSTITDTVIKQRGELLSLGVIMMLFFSSNGIIALIQAFNATYHSLETRSGLNRRFIA
                     IMLVLIISVLLTSAIVLVTASDLLMKALLSVEFLKSAWVYFFISVIRWVIVIALFFFS
                     TSFLYYLGPSRRNRFRFISPGATLATIIQIFATLGFSYYIENCGQYNKLYGSIGTVMV
                     VMLLLYLTAFALIIGFELNASVQQSDTSKRKPIKKK"
     gene            complement(13194..13577)
                     /locus_tag="PLK75_04465"
     CDS             complement(13194..13577)
                     /locus_tag="PLK75_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38532.1"
                     /translation="MKKIIVIAIVLVMTGSVSLAGNNFDFSNNQNSSIEKADASLKPD
                     DLHTSGTITRLHVPWAKNGLAISEMVISKGDYCLYRVASDNLINIPSCEPVGTQYHYF
                     VYKNGSFLFTVNQCNKSNVYRFFSE"
     gene            complement(13751..14365)
                     /locus_tag="PLK75_04470"
     CDS             complement(13751..14365)
                     /locus_tag="PLK75_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38533.1"
                     /translation="MRTIAFVFLIVLQLSQAVYAQNKIFLLNGKSITTDTLSYDFPAN
                     IIYYNNPDRHKTKMIDGSEVFSFVDAQNRETVVYTQADKESLSIAEMRSVMQGQAVAL
                     NEYNPIWPYLAGFGVGCASMCLPVNSMVSITIPIGYNVGAAFMRPSGSHIMKKYPDLY
                     TDDLFVYGYKERGGNKILKRSVIGSVAGIAVGFTGMFLISTLSK"
     gene            14482..17346
                     /gene="gcvP"
                     /locus_tag="PLK75_04475"
     CDS             14482..17346
                     /gene="gcvP"
                     /locus_tag="PLK75_04475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOY38534.1"
                     /translation="MNNFVKRHLGPRESEIPEMLKVVGVKSMEELIEQVIPKSIRLPE
                     EISLPEPVSEYEFLKVIQEIASKNRICRSFIGRGYYGCDMPSVIKRNVFENPGWYTSY
                     TPYQAEISQGRLEALLNFQTLITELTGMQISNCSLLDEATSTAEAMSMMLELRSRQAS
                     KEGRNVLFVDKSLFPQTIDTLKLRSEPIGVELVIGDFETVQFSDKMFGAIVQYPSETG
                     EICDYSAFTEKAHQHEILVTAVVDLMSMVLLAPPSEWGADIVTGCTQRFGLPMYFGGP
                     HAGFLATRDDYKRKMPGRIIGVSIDKFGNRALRMALQTREQHIKREKATSNICTAQAL
                     LATMSGMYAIYHGPEGLKEIAGRIHSAASIIANGLNNLGYKVNNKNFFDTLHITLPEG
                     IKSCDIQVLANDKNINLFYINEKTLSISTDEVVTVDEVNTLLHIFAKANNKPFTAVTC
                     IENKKAFELKFSRKSDFLQQQVFNSYHSETEMMRYIKKLERRDIALNHSMIPLGSCTM
                     KLNPATSMLPLSWDSFCNIHPFAPADQWKGYKQMIEELEKDLAAITGFASISLMPNSG
                     AAGEHTGLMVIREYHKNNNQAYRNICLIPASAHGTNPASAVMAGMEVVVVNCDDKGNI
                     DVDDLSLKAEQHKDKLAAIMITYPSTHGVFESRILEMIDIVHKNGGQVYMDGANMNAQ
                     VGLTNPGFIGADVCHLNLHKTFAIPHGGGGPGVGPIGVASHLVDFLPNHVMMKTGGAK
                     GIHAVASAPYGSAGVLAITYAYIRMMGTAGLKKATEMAILNANYIASSLTGYYKVLFT
                     GETGRCAHEMILDCHNFKERYGVEAADIAHRLMDYGFHAPTLSFPVHETLMVEPTESE
                     SKQELDRFIETMKSILAELEDIYTGKADKIDNPVKNSPHTQVECTSDEWNHPYSRQQA
                     AFPLEWIKENKFWPYVAKIDNGFGDRNLVCTCEPIETYMK"
     gene            complement(17414..17920)
                     /locus_tag="PLK75_04480"
     CDS             complement(17414..17920)
                     /locus_tag="PLK75_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY38535.1"
                     /translation="MLKIGLLSDTHGYVEPAIYEFFSDVDEIWHAGDMGNVSVADTLK
                     SFKPFKGVFGNCDGYDVRIEYPGSLRFVAEGLSVWITHIGGYPGKYDPRVKPEIFLNP
                     PRLFISGHSHILKIMPDKKLSLLHINPGAAGNRGIHKVKTLLRFQIDNATIRELEIME
                     IKRSNSDG"
     gene            18043..19428
                     /locus_tag="PLK75_04485"
     CDS             18043..19428
                     /locus_tag="PLK75_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOY38536.1"
                     /translation="MSLIILLIVLISLMVAVLVLTIVFRPSQHNENVAFDNYFKDLQK
                     QINQFEAGLKNEFRINREESAAISKENRTELSNTLKDFRQELSVITKNIADQTTNNNK
                     LLRDEIALVFKDFNENFTKNIQSMNDMQREKFSQLDERQVKLIESTEKRLDQMRETVD
                     EKLQKTLNERLGQSFETVGRQLKEVQEGLGEMRTLAQDVGGLKKVLSNVKDRGGLGEL
                     QLEMLLEQILAPEQYAANVKTRHDSSEIVEFAIKLPGRDDQKSHVWLPVDAKFPKDAY
                     EQLLVAYDTADNSIIETAGKNIEATIKKMAKDIHDKYIDPPNTTDFAIMFLPFEGLYA
                     EIIRRSSLMEQLQREFKVVVTGPTTLAAILNSLQMGFRTLALQKRSSEVWTILGAVKT
                     EFEKFGDLLSKAQKQIATAGETIDQLAGTRTRAITRKLKEVQALPAGEAEHVLPEIAQ
                     DEIIDEEETIS"
     gene            19452..20159
                     /locus_tag="PLK75_04490"
     CDS             19452..20159
                     /locus_tag="PLK75_04490"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOY38537.1"
                     /translation="MQYFISNIKAFEDIGILNKTESQHCIRVLRHKPGDKILVTNGDG
                     LLLEATILTADPGKCSFHVVRQLESPKQHPYYLHIAIAPTKNTDRLEWFVEKSVEIGI
                     NEISFVSCRFSERRAVNIERMLKIAESAMKQSLKTRLPVINGIVPFTDFISKRSIGLK
                     CIAHCMGDKKVHLTESLNSGHNHITVIIGPEGDFSVEEVEAAIKQNYYPVSLGENRLR
                     TETAGLVACVIIASKLN"
     gene            complement(20156..20857)
                     /locus_tag="PLK75_04495"
     CDS             complement(20156..20857)
                     /locus_tag="PLK75_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38538.1"
                     /translation="MRKLCTILIFSLVTLCAQAQAVVYDKNDGESYYNGTEWGPEMRH
                     YSYYFVRYNMAVPLQQPVGEMPLGSGNWAIGISYKLKVVSIWDIGVDLSYENEFHRMK
                     NLPNTRSIVPEVSTFDKVRTYQNNITGAFYTRFYFKQNRDHDFGWYIDGGAYYSYVAG
                     WGFVKKNNTKLERIKLRSKRDVELENQNYGVFLRMGYNQFAIFARYNMADVFANEEVV
                     VTPFSVGLQLNLVMF"
     gene            complement(20972..21832)
                     /locus_tag="PLK75_04500"
     CDS             complement(20972..21832)
                     /locus_tag="PLK75_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOY38539.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEELISVEEEVELAQRIK
                     KNDRAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPTSEELAK
                     ELDLPVEKVADTIRVSGRHVSVDAPFVSGEENSLLDVLENSDSPHTDSTLINESLSRE
                     IDRTLATLTERERDIIRYFFGIGCQEKTLEEIGEEFGLTRERVRQIKEKAIRRLRHNS
                     KSKLLKAYLG"
     gene            complement(22024..23301)
                     /locus_tag="PLK75_04505"
     CDS             complement(22024..23301)
                     /locus_tag="PLK75_04505"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOY38540.1"
                     /translation="MTKSYLLFMCSLMLVPTIMNAQETYTAFFGNLHSHTSNSDGEGE
                     PEDAFEYARYTAGIDFLAVTDHMEQIDMLEWYNVKNDADDATENGSFVAIAAWEWGSP
                     LYGHINVFNTDEIISDVLNLWYYNDFDEFLDWLSENPPSIAQFNHPGDEETFNNWNDF
                     EYVGESTDEIFALIEFQNVQQATDWYEFALSKGWHLSPVWNQDNHSADWGNKNNGRAG
                     VWANTLTRAELFEAIIAGRTFATMDKNASVWIDINGVPMGGSLPVDHTMSLHINLTDT
                     DVENYASIEVVSQNGIIHSITNFSGDFNAVIDISLGNDQWVFVRAVQTDGDYLWSAPV
                     YLPGDVTGENIMQFQNEIKIFPNPASGVVRIEAGNITGNLDISTIDGKLVYRDALFNA
                     VTTLNTNMFQPGCYLVRLQTEDSQYKSKLIVVK"
     gene            complement(23404..24858)
                     /locus_tag="PLK75_04510"
     CDS             complement(23404..24858)
                     /locus_tag="PLK75_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOY38541.1"
                     /translation="MEKKKFLLGLAGGMLGGIVVLTAALITDSLGSFQSNEKHPLVQQ
                     YDYSHKNVPVQKTGLFSQSEFSAPDFTDAAEKAVNAVVHVKTQYNAPNYTLYDYFFGT
                     APRYSTPVMASGSGVILSSDGYIITNNHVIENAEVIQVVLNDKRLFDAKLIGKDGASD
                     LALLKVEANDLPFIPYANSEDVKVGEWVLAVGNPFNLTSTVTAGIISAKARNINIMGQ
                     SMDIESFIQTDAAINPGNSGGALVNLNGELIGINSAIASRTGSFVGYSFAIPSNIVRK
                     VVADFIEFGDVQRAYLGAEFADIDADLAKSLKMERPIGIYVAGVWSDGSDSKSDLKKG
                     DIVLEVDNHKVNSTAEMLEQLSKFRPGDRVEIRIIRNGLPYTVNAMLTNKYGTTDIVK
                     ARSVEILGANFEPVPDADKQKLRIRNGVKVKSLSSGKLLSRGVQPGYIITRINNRNID
                     SVDDVEDALKNSDGAVFIEGIYPNGTVAYYAFGL"
     gene            24962..25753
                     /gene="dapF"
                     /locus_tag="PLK75_04515"
     CDS             24962..25753
                     /gene="dapF"
                     /locus_tag="PLK75_04515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOY38542.1"
                     /translation="MDISFSKYHGAGNDFVIIDTRKSSLNLNSNQIKQLCHRRFGIGA
                     DGLMLLQNSAQETFKMKYYNSDGIEGTMCGNGGRCIVAFASDLGLLGTGTKTSFEAID
                     GVHHAQKLGHNNISLCMNSTQIPVRFEDGFFVDTGSPHFVKFVPDISQIDAETEGRLL
                     RFEKRFAPKGTNVNFVQILLTGLRIVTFERGVEAITLACGTGAVAAAVCFAELENKNS
                     LQIPVISAGGELFVTLEKKPDGYHNIVLSGPAMRVFTGTFSLVSE"
     gene            25802..27142
                     /locus_tag="PLK75_04520"
     CDS             25802..27142
                     /locus_tag="PLK75_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOY38543.1"
                     /translation="MKPTFLFAIFSCCILIVACKSQSGTSENNKPDSNASNVTHPIQG
                     NTDSQVATTDIEKPKPEGYDKNISLPQNLTNFSFDFYKFLSEESKGKNLCFSPVSMNI
                     ALAMVYEGARNNTLEQMSQTMHLPDNQELFSNEFASYLRSIELLAKDSAVEFNLANRV
                     FVESSFPILESYRSAIKQAFNGAFEQMDFINKFAESEQYINKWVEKMTKDRIKELLPA
                     GTLSSETKLVLVNAIYIKARWKYIFDEKATMKKDFHINGSNIVQTDFMIRKQKGIRYY
                     TNNDICAIELPYTCDGISLIIIKPHKGSSENLSSFIPTGSDYEKMLSDMKYHTVHMEI
                     PKFKTETSFSLSDPLVAKGMSDAFSPRADFSGITGNNDLSVSEVLQKVFFEVDEKGSE
                     AAAASAVVMRATSAAPNHEMEEIMYFIANQPFIYILKENNSDTPLFIGQYISPQ"
     gene            complement(27200..>27497)
                     /locus_tag="PLK75_04525"
     CDS             complement(27200..>27497)
                     /locus_tag="PLK75_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38544.1"
                     /translation="LSDDNNSVPWFRGMGYDEANALRKFEYINIQTNTYTDSIGNAAV
                     FITGKTESDGFTPQSRIEYIGSDISKIVLKYTVTNNAVTPPTVDQVLEEYTPVD"
BASE COUNT         8563 a         5740 c         5268 g         7926 t
ORIGIN      
        1 ctgatggttc gtgcatccag gtcaaaatcg tggctgcggt tctgcaattg attgtcgatg
       61 agagctgcca gcaggttatg tgctttttca acagccgata agtcgccggt aaaatgcaaa
      121 ttgatgtctt ccatagggat tacctgagaa taacctccgc ctgcagcacc gcctttaact
      181 ccaaaaacgg ggcccagaga gggttcacgc agtacaacgg tagttttatg accaagaaga
      241 ttcatagcct gagccaaccc gatggatgtt gtcgttttcc cttctccggc aggggttggg
      301 gtaatagcag ttaccaaaat taacctcgaa ttaagcattt tcgactcatc gataaggctc
      361 agcggcaatt ttgccttata tttaccgtat aactcaagct gaccttcggc aatacccaaa
      421 cttgccgcaa tttctataac cggcttaata tcagccgcct ttgctatttc aatatcagaa
      481 cgtatcataa ttttccttgt ttctttaaca aacaatattc atgttgtttt gtttaataaa
      541 accttagcat gggaatactc atagaatctg tagctattcg accaccaatt tccgattaac
      601 tgtgtcatcc ccagtaatta ttcttacaat ataaacaccg cttttcaaac cactgatgtc
      661 gataggttga cgggaataaa aattatcacc tgaaaaaacc agagtcccgg aagcattaag
      721 tattgtaata ccaaaacctt ctgcacaatt tacaactata aaattctcct taacaggatt
      781 gggataaatt tgtattgcga gaagggtgtt attgtggatt gccgaaacaa ccggagtaaa
      841 tacgtactca caaagactgt cgatactgtt ggttatttta atgtgcaatt caaggctgtt
      901 ttcatcaata ttgtctgttt cgaatgggat tgttttaccc tcaaagctct cattatcgat
      961 taaccagtaa tatgacacat ggtcgtagaa aagagattcc aaatcttcaa ctccggtaac
     1021 cgttagcgag ctgccatcga aataaacaat tggctccggg aaaacataag ttatacatat
     1081 ctgaggccaa tatgccggat cgttgtaatc gcggctggca aaattcattt tatggaaata
     1141 agagtccaga tcttcgtccc attgcatcag taccaaccca tgaaaattac tttccagcgt
     1201 atggtcatcg gtcagtacaa cattcgtaat atcaatattt aaaatatcct cataactatc
     1261 agtgtttggc gaatcgggga taggggaaat ttcagcatat tcatcagtct caatatcggg
     1321 ttgattgttc catgtgatag cagttgaaac ccagtattcg ggcactttat atattcttgt
     1381 agtgttgatc tgcccttcac cgtagcgatg tgcaaaagca gtattgctga acgggttaaa
     1441 cagattcagg tgacacgaat atatgtaggt tgatgtcaag tcaatcaagt tctcaaagtc
     1501 gttagccagg taggctctct tttttcccgg cgaaccgttc catgtccaga cttgagtatc
     1561 aagaaagttt gcagtggaat aattttgcgt gtttgtaggt ccccattggg ggttattctg
     1621 attggtcaga taccatacca ccgcatcttc tttcggataa taaacctgag tacacgaaaa
     1681 ggtctgagca gaggaaaaac tgctggtaat accaagcgat aaaaatatca agataactcg
     1741 caacattttt ttgttatttt tgagcaatta tatgataagc ttcaaatgta ctaattattt
     1801 tcatatgatt aattttaata tacaaactaa atagatatta gcaatgaaat tacgcccact
     1861 ttttaaagga ttgtttacag tttgcagggg gcattatatt gttccgggca actgctgtgt
     1921 ttttgttggt cagatgtcgc ggctttcaaa atggattcat aaacacaaaa gaattgcagg
     1981 ctattccgat ttttacgata tacatttcag atacaacaaa agggaagatt tattcagata
     2041 cctgatagaa aatgaaaatc tggatgcagt tgattacctt gagttcggtg tagccaatgg
     2101 atgctctttc aaatggtgga ctgaaaacaa tacaaatacc gactcacgtt tcattggatt
     2161 cgacactttt accggacttc ctgaagactg gggacccttt aaaaaaggag atatgtcggc
     2221 gggtaatgca atgcctgaaa ttaccggcaa caggcatcag ttcgtgcagg ggttgtttca
     2281 gcaaaccctc cctggctttc tgaaatcatt tgattttaag aatcggaagg taattcacat
     2341 ggacgctgat ttgtactcgg ccacactgtt tgtactcaca agcattgctc catatctaaa
     2401 caaaggcgat attatcatgt tcgatgaatt caacgtacct atgcatgaat ataaggcttt
     2461 caccgaattt gtaagttcat attacatcga atatgaaatt ttgggagcag tgaacaactt
     2521 ctatcagatt gctataaaaa taaagtagtt ttctaacgga acacttccat tcggttggcg
     2581 tcaatccgta agaaaatcat tagcagcata gtaaaagccc atagcgaaga gcctccatag
     2641 ctgaaaaacg gtagcggaat cccaattacc ggagccagac cgattgtcat accaatgtta
     2701 atggccacat gtataaataa aaaggaagcc actgaatacc cataaatacg tgagtaggct
     2761 gatcgctgcc gctcagccag ccaaataata cggattatta aaaaagcgaa gagcatcaga
     2821 actatcatac taccggcaaa cccccattct tcgcctactg tgcaaaaaat aaagtcggta
     2881 tcctgttccg gaacgaagtt gtattttgtt tgggtgccct gcaaaaaacc tttgcccagt
     2941 aagcctccgg aaccaatcga aattttcgat tggttcacat tatatcctgc tccgagagga
     3001 tcggaattaa accccaacag ctcccctatc ctctcctgtt gatgtttctc aagtagattg
     3061 ctgtatacaa aatcaaccga gtaaacgtaa agcgatgccg ccagaagaat gaccagaacc
     3121 gaagctgctt tgtcgatttt ttttaataat gcaataagga gtgaaacacc tatcgagaga
     3181 acaaccgccg acaaaatcaa atacttcaac gggtacggcc atatcccaaa atattgaacc
     3241 aaaattgccg ctgcagcaaa cacagcaaac acaagcaatc cccaataccc ataccacctt
     3301 ttccgcgacg tgaatataaa atacagtatc gcagccaata cgagtaccgt cattataatc
     3361 agctgatttg aaaccagtgc aaaaacaaac agagcaattg ccgcaaaaaa caacacaaaa
     3421 agcgtgttat tcaaaccttc gcgatacaat acgaatatga ggctgctgta tatcagggca
     3481 gatccggcat cattctgcag aactatcaac cctatgggta agccaatgat aagtattgta
     3541 cccaacacgt cctttaaatt attcaaacta aaatgagttc gacttgcaaa ctgcgccagg
     3601 gcaagcgaag ttgccatttt tgcgaattct gagggttgta acctgaaatc tcctatctga
     3661 aaccacgatc tggaattgtt aatctctgta ccgaacaaca gcactgcaat gagcaacagc
     3721 ataaaaaata caaatatcac atacgaaaat gccgaataga atttactgtc gatgagaaaa
     3781 acaaaaaagc ctatgataaa agctgcagct atccatataa gctgctttcc gtagcgcatc
     3841 gaagtatcga agatgctggt atgcgattcg tcgtatacag cggcataaat atttacccag
     3901 cctgcaaata ccagaagaag gtagagtgca atacatatcc agtcgatatt aagccatatt
     3961 ttacctcgcc gtgtctgcaa aatgataaat atcagtgttt aaaattcgtt gctctgaaaa
     4021 tacattcatc acagtatcgt taatatattt ctcaatcata agactggcga taggcgcagc
     4081 ccatactccg ccgaagccgg cattttcgat gtatactgca atggcaatct gagggttttc
     4141 tttaggggca aaagccataa atatcgagtg gtcttcacca tgtgggtttt gtgcagtgcc
     4201 ggtttttcca cagactctta tattgggaat ccttgccaag ccaccggttc cataatcctc
     4261 atttaccacg gcatccatac cttcaacaac ggggataaat acttcgggtt taaagggagt
     4321 acgtatggga attgtgtaca tggtgtcgag tggagcacct cctatcgatt tcataatatg
     4381 cggataataa taaaacccac ggttggcaat gattgcagca aaatttgcca tctgcagtgg
     4441 aaccacaagc acttcgccct gaccaatgct taatgaataa attgtgctat aagcccaccg
     4501 gtatttacca tatattctgt cgtaaaaatc aggaccgggt attttaccct ttaccaccga
     4561 gggcaatcca atatcgaaag cgcactcaaa acccaggttt agcacttttt ccttccatat
     4621 tgccaaacca atccggctat cgcggaaaat gcttttttca aaaccccgct gtatcaattt
     4681 acgtgtcacc atataaaaat acggattgca cgaatattga atagcacgtt ttaaatcggt
     4741 attgggtgga tgattatggc agttcactat gcttttatcg caggggaagg atgtttgttc
     4801 tgtaataatt ccctgatcga gtccaataat agcctgcacc attttaaata tagatcccgg
     4861 aggatacgaa gctccaacgg cacgattgaa caatggcttg ttgggggcat gtaccaggct
     4921 atcgtagtta taaccccgct gacgtccaac aaggagattc ggatcgaagc ccggactgga
     4981 aaccatagcc aggatttcgc ctgttgaagg ctgaatagca acaatgcttc caattttgtt
     5041 tttcatcagc aattcaccgt actcctgaag cccggcatca attgtaagaa caaggtcttt
     5101 gcctgcttgt gcaggaatat cgtactttcc ctgctgatag ctgccctgaa tacggttgtg
     5161 aacatcaacc atatatattt tcactccctt ctcgcccctc aatgaggttt cgaagtattt
     5221 ctcaactcca cttttgccaa cgtaatcacc aatatagtaa aaagtatctc tttcgagagt
     5281 tggcatatca atctcaccaa tgtcgcccaa tacatttgaa gcaaaacttt tttcatattt
     5341 tcgaagcgaa cggctttgta cataaaagcc tggatagcga tacatatgct cctggaaata
     5401 agcatactgc aatttcgaca tctgtttaaa aaaaacagaa ggccggaata ctgaataagc
     5461 agctgctgca gtcagtttct gacgagcatc ttcaatattg atgcccaact cattcaccag
     5521 ctgtaacgta tcgaacgacc taacctgcct cggtataacc attaaatcgt aagctatttc
     5581 gttgcataca agcaattcgc cattacgatc gtaaatcagc cctcgtgatg gatattccca
     5641 gattcgccgc tgcgaattgt tttcggatga aattttatag gatggatcaa gcacctgaat
     5701 aataaaaagc ttgacaagga ttgccagaaa tatcagtaaa attattactg taatataaat
     5761 acttcgtgaa cttagctggt tcatcgcctg cgaattgtta aatactggct tagaattaac
     5821 acgctgattg tggaaatact gctcaatgta atacgcaaca aggtaaattg aaacatttta
     5881 aagctaaatg catcgagcat aaagagtaaa aaatgatgta taataacaag gctggctgca
     5941 tattttagaa accacagaaa acccagatcc cgaatcccga tatgtgtgtt cgtttcatat
     6001 ccatcgcgcg gtgagtacaa acgcaacatc cctggtctaa tataggctgt cagaacactt
     6061 gcagcagcat gaattccaaa ggtattgctg aatgagtcca caactaaacc ggtagccatg
     6121 gctagcaaca tgagcaaaaa cggaggtgtt tcgattggaa gaaggaggat gaacaaaata
     6181 tagaaaaaaa catgtgtata gggtatcgat tgtatctgat tgaacaataa tacctgcaaa
     6241 gcaatgcaga taataaacag aaaaaagtat ttcagattac tgtttatcat tttgcgcctc
     6301 caatgcttct atttcttgtt tatgcaaatt ttttaccaca tacacatttt tgagattggc
     6361 aaaatttgta gccagtttaa catcgatttc gtaaaaacca gttgatgtca tttttttata
     6421 actatcgacc acacccacag gaatatcggg tggaaaaata gatgaatatc cgctggtgac
     6481 aacagtatct cccactttta tatcaatata taccggtatt tccgacattc tggcgtagcg
     6541 atggcttttg cctgtccacg acaaagatcc aaagtagttg ttcctgctga ttttaacact
     6601 aagtttgaaa gccgtattaa ttacaggcag cacagtagaa aaattatcag agcaattgta
     6661 aacaattcct gcaacaccct ctggaccaat tactcccatt tctttacgaa ctccctgttt
     6721 taatccggca tcgatggtaa tatagttttt tacattgttt acactgttgt ttattaccgt
     6781 tacattcata aatgtgtaat cggggtttac agaatcgaat acaaagcttc gattataggc
     6841 acggcgctgc tgaagttcat tctgtaatgc agcattctca atggcaagtc ttcggttttc
     6901 gcctgccaat tttacatagt cggtaacaac cgaccacgat ttttcgatgc gactgattac
     6961 cacatacgac gagctggcaa aaaccgaacg ttgatactga ttgtttgcaa cgagtaatgc
     7021 aatacaaatc aattcaagaa tgataaatag aaatgtagca tgataacgta agataaatcg
     7081 gaataggctt ttcatctgta gttgttaact atctgatgag gaactgaaat ttatcaacgt
     7141 ttttaagtgc aataccggta cctcttgcaa ctgcatgcag tgggtcttcc gaaacatgta
     7201 caggaatatt ggttttttcg cttaaccgtc tgtcgagtcc acgcagcaat gctccaccgc
     7261 ctgccagata gataccgcgc atataaatat cggcatataa ctcaggagga gtttgttcca
     7321 gtaccatcat gattgctgtt tccaccttcg aaattgactt atcgaggcta tgcgcaatct
     7381 cctgatacga tacaggaatc tcaataggga gtgctgtcat ctggtgtggg cctctcacga
     7441 tgtagtcggg aggaggattg tcgatatttg gcaacgctgc tcctacatta atttttatct
     7501 cttcggccgt acggtctcca attctgatgt tatgctgatg tcgcatatac tcctgaatat
     7561 catcggtaaa ttcatctccg gcaatacgta tcgaccgatt gcagacaata ccgccaagcg
     7621 aaattacagc aatttcggta gtaccaccgc caatatcgca taccataata ccacccggag
     7681 cctctacatc catgccaata ccgatggcag ctgccattgg ctcataaatc atataaacct
     7741 ctcttgcgcc ggcatgttcc gaagagtcgc gcacagcacg tatttctacc tcagtgctgc
     7801 ccgaaggaat acataccacc atcttcagcg atggcgtaaa aagtggccgc tttttgttca
     7861 gcatttttat catccctcta atcatcaact ctgcagcgtt gaagtctgca ataactccat
     7921 cgcgcaaagg gcgaacagtg cggataccct gatgggtttt accatgcatt tccctcgctt
     7981 ttttaccaat ggcaattagt ttccctgtgc gttcatcaat cgctacaatc gatggctcat
     8041 caaccacaat tttatcatta tgaatgataa ttgtatttgc tgtaccaagg tcaatagcta
     8101 tttcctgttg taaaaatgaa aacaatccca tgttattcca cgtattttaa attatcattg
     8161 cattcagcca ctgtagcttc tttacaatta agtgggtaaa gataataaca attcaacaat
     8221 tttattaaca ttatttattt ttttcatacg actgagcaat tatttttgcc ctcagcaatt
     8281 tgaatgtaaa ctactcgctt ataaccagtt ccgttaaaaa cactttaata acggaaagca
     8341 gaatactcat cagagccaag actctaatca gtgcaaggaa tgtttttagc agcaatacaa
     8401 actacaagga gaatatatag gctgtgagat tggcttcagg tttcaaatta agcttgcgtc
     8461 gcaatctcgt tcttgcaacc cgaacactgt cggcgctcag gtacaaaatt cccgctatat
     8521 gttttgtaga aagattcaac ctcagtaagg ttgctaactt aatctcagaa ggtgtaaggt
     8581 tcgggtgctt cgaaaccaac gcgttttgaa agtccgggtt cacactgttg aaatgatctt
     8641 ccaattgctt ccaggaatcg gtatgaagtt tattttcgat gacttcgcgc atttcaataa
     8701 gtctcgatac aggaacagtc tgcacatcgc cttcaccaat catttgatta agcatctgaa
     8761 tgagattaat attaaattcg ttacacttaa ctatctttat tgcactctgc accaattcaa
     8821 catctttctg ggcaagttcc cttttatgat aatccaccaa tttcaacaat gagcgcacac
     8881 gagcccggag ttcagtttca tcaaaaggtt tccttataaa atcgactgca ccggcattca
     8941 acgtgtttaa aacatagtaa tacgacttca ttactccggt aatcattaca accataatgg
     9001 gagaagttac ggggtttttc tttagtttta ccagcgtagt gtacccatcg ttaacaggca
     9061 tctggcaatc aagaataatt aaatcaggtt tttcagctgt agctatttct atccccgacc
     9121 agccattata agcagccaga atttcgatag gttcattgct cagaatagtt ttaatcgcgg
     9181 taatatttac cggttcatca tcaattacaa gaattttata attactcatg cctgaaagat
     9241 tggtttcata acaaatataa ttattatatt taacatttta atgtactaaa aaacaaaaac
     9301 aataaattaa cctttgtaaa taacatttat tatataatta acaataatat ttatactaga
     9361 atcaatttat tattaaaagt actcccaatt tgataactca atacatctta tgaatagtga
     9421 agaaaaacca cataattcgc aaaacgaggt gaatttactg caaaggagtt ccgataaaat
     9481 gcttgatgca ttcgaaagta tgctgttcca tgaattgatg aatcacctga cttttgtagt
     9541 ttacccaccc gatagcctcc ctgccgaaga aagcctggag ttttcgaagt tttcaggaaa
     9601 acagattctc actatgctaa atactatcag atatgtaaaa ttatacgaaa agaacgaact
     9661 tagggcactc ccctctgcta ttcatcccgg acaattattt gaaaaaacta taagcgaatt
     9721 caaaccgatt acatcaggta aaaatcaaac atgcactttt gagcatgatg gtattgataa
     9781 aataattgct gatgaagagc ttttaaaaat atacttttgg aacatcctga gtatatttac
     9841 caaatatgcc cctgagaaaa gcattttcga ggtaaaaact actgcatccg aaaacttctt
     9901 gactcttgac atcagtcatc ctcactctta tgcaggcgaa tattataaag ctgtcaattt
     9961 tgataatttc ggtgacgcat tgcttaataa tcaggggttt gaaagaagca ttgcgattaa
    10021 actacttgcc tgcaagtata tgatggccat tcacaatgca gcgtttcgca ttttttccat
    10081 cggcgataga acaaactttt cagcgaggtt cagcgggttt gacgctgaca aacgcgaaaa
    10141 tgacaacaat gatgaaaaaa acaagaatat tcggcttgat aaggcaggaa tgaaaaaaac
    10201 agaaccacta attcaggctc ttaaaaacac cgatttttat aaaattaccc gtatatacga
    10261 catactatcc gaaattgaaa tggctgatcc atgcgaaaac actcaggcat gggcaaaaac
    10321 ggccaaaatg gtattagaat caggaaagaa agatgatttc gaagcatttc tcgataacac
    10381 cctgaattat tttcactcat gatgatacgg ttcatttgat agaatggtaa acgcacgata
    10441 taactgctcc gcaaaaatca gtcgtatcaa ctggtgcgaa aacgtcatcc tcgacaggga
    10501 aattttatca ttcgcggctg catatacatt tttcgaaaat ccgtacggtc ctccgataat
    10561 aaagcacagg ttacggcaac ctgcaaccat ttttttattc agataaccag caaattccac
    10621 cgacctcaat tcaatgccgt tttcatcgag taacacaaca taatcgtcgc ttccgatgtt
    10681 tttcaatatc tgaactcctt ctgcactttt caattcagtg acattcgttt tttttgattt
    10741 aatctccgga attataataa gtttaaaatc gatgtatcga gatatccttt taaaatacaa
    10801 ttcggtacct tccgtcagat agcctgcatc agtttttcct gtcagtatga gcgtaagctt
    10861 catcgcaata aaaataatcc ttgaaattag ttttttattt gtatattgcc aactattgat
    10921 ttttggtttg atatttgcaa gttttttgta aatttttacg tatgaaaatt atcagagcat
    10981 cgtttttatt ttgttcactg ttctttctaa catcgcaggc ttcacaggca tttccgatac
    11041 ttgatattcc tctgaagatt tttcaggcaa tgaaagttgg caatgtcgat gaattgtcaa
    11101 aatacttcaa cacatcgctc gagttggttg tgctcgataa ggaagatgtt tacagcaaac
    11161 aacaggctgt gcagattttg aaagacttct ttacaaaata caaagccaaa aattttacat
    11221 tgcttcatca gggtgggaaa gaagaagccc aatatgccat tgggaccctt gaaaccgaaa
    11281 aggggaactt cagggtatat tttctggtta ggcaaaaagg agcccaaaca ttaattcatc
    11341 aacttcgaat tgaagaagaa tgattggcaa aacctatctt ttacaactta ttaaaagagc
    11401 attcaccgaa gaccttggtc ctggcgacca cacttctgaa gcgtgcatac cttccaatgc
    11461 ctttgggaaa gtcaacttac tcgtaaaaga gccgggaata attgctggcg taaacattgc
    11521 caaagaagta tttcgatttg ccaaccccgc tatccgggta aacactttta tcgaagatgg
    11581 ctctcaagta aacacgggag acattgtttt tactgcagaa ggctctcaaa aatctttact
    11641 cacagcagaa agaacggttc tgaattttat gcagcgaatg agcggtattg ctaccgaaac
    11701 acgccggtat gctgatgcag taaaaccgtt taacacacaa atacttgaca ctcgtaaaac
    11761 aacacccggg ctaaggctta ttgaaaaaga agccgtcaaa atcggtgggg gcacaaacca
    11821 tcgtattggg ttgtacgata tgattatgat aaaagataac catgtcgatt tttcaggtgg
    11881 tattgttcag gctattgaaa atgttcataa ttacctgagg gctcacagtt tgtatctgaa
    11941 aatcgaaatc gaagtcagaa acttcgttga gctcggagaa gttcttaatc acggaggggt
    12001 cgacagaata atgctggata atttcagtat tgaaaacaca gccactgccg tagaacagat
    12061 tgccggccgc tatgagacag aatcttcagg aggcattaca ctccaaaata ttgccggcta
    12121 tgcagcttgc ggggttgact tcatttcagt tggtgcatta acgcatcata tcaagagcct
    12181 tgatttaagc ctgaaagcgc tgtaatgaaa attccatatc aaaataaact actacgaaaa
    12241 ttacaaaaac cgatttcatg gctaaaaagg cgatcgttcc cgggttttga cggatacccc
    12301 atctacgatg tcggcgcatt tttcgtttcg ggattaatga agggccacct cggactgcgc
    12361 gctgcagggg ttgcttttaa catttttctg tcgatttttc cattgataat tttctttttt
    12421 acgcttatac cctacattcc aatcgagaat tttcaaactg agctactaca atttatcaat
    12481 gatgttcttc cgaacgaggc caatacactg tttaactcaa ccataaccga tacggtaata
    12541 aaacaacggg gagaactctt atcacttggc gttattatga tgcttttctt ctcgtcaaat
    12601 ggcatcatcg ccctcattca ggctttcaac gcaacatatc acagccttga aacccgttcg
    12661 ggtctaaatc gacggttcat tgcaatcatg ctggtattaa taatttcggt attgcttact
    12721 tctgccattg tacttgttac agccagcgat ttactgatga aagcactttt aagcgttgaa
    12781 ttcttaaaaa gtgcatgggt ttactttttt atcagtgtaa tcagatgggt aattgtaatc
    12841 gctctgttct ttttttccac atcgtttctt tactaccttg ggccctcacg cagaaaccgt
    12901 ttccggttta tttcacccgg agcaactctt gcaacgataa tacagatttt tgcaacactt
    12961 ggcttttcgt attatatcga aaactgcggc cagtacaata aattatatgg ttctataggt
    13021 acggtaatgg tagttatgct tttactttac cttaccgctt ttgccctaat aataggattt
    13081 gaactaaatg ccagcgttca gcaaagtgat acttcaaaac ggaagccaat taaaaagaaa
    13141 tagtccagtt ctcaagctat caatacataa caatatcgca aacccagaaa taactattcg
    13201 ctgaagaaac gatagacgtt tgatttattg cattgattga ctgtaaataa gaaactcccg
    13261 ttcttataaa caaagtaatg atactgtgtt ccaacgggct cgcaactggg aatattgatc
    13321 aggttgtccg atgccacccg atacaaacaa taatcgcctt tagaaataac catttccgaa
    13381 attgccaggc cgtttttagc ccatggaaca tgcaatctgg ttattgtgcc tgatgtatgt
    13441 aaatcatctg gttttaatga tgcgtctgct ttttcgatac tactgttttg attgttggaa
    13501 aaatcgaagt tgttacctgc tagggaaacg cttccggtca tcactaaaac gattgctatt
    13561 acgatgattt ttttcatgac tgattttatt tggtttcaat aatttaacta ctacaaaaat
    13621 aagtattgca aaagccccat acagttaagt ataggttaaa ctatttcgca attccgttaa
    13681 acttggttaa tgaacgttaa caagtgttaa aatgtaaaaa acaggcacat tggattggga
    13741 gaagagcgga ttatttcgac aatgtcgata tgagaaacat acctgtgaag cccactgcaa
    13801 ttcctgcaac agagccaatc acacttcttt tcagaatttt atttcctccg cgttctttat
    13861 aaccataaac aaaaagatca tcggtataca ggtcggggta ctttttcatg atatggcttc
    13921 cggatggacg cataaatgcg gcacccacat tgtatccaat aggaatggtg attgaaacca
    13981 ttgagttaac aggtaagcac atgctggcac accctacacc aaaccctgcc agatacggcc
    14041 atataggatt gtattcattc aatgcaacag cctgaccttg cattacactt ctcatttcgg
    14101 caatgcttag ggattcttta tctgcttggg tataaacaac tgtttcccga ttctgagcat
    14161 caacaaatga aaaaacttcg gaaccatcaa tcattttcgt tttatgacgg tccgggttgt
    14221 tataataaat gatattggcc gggaaatcat agctaagcgt atcggttgta atacttttcc
    14281 cattcagtaa aaatattttg ttttgtgcat aaacagcctg agatagctgc aacacaatta
    14341 aaaacacaaa agctatagtt ctcatattgc tattttatta caaaaaaagc aaaatattga
    14401 caaatgacaa tataatgtga tgcagtattt tttattttta cccataatta aaaatatttc
    14461 acaaacaata atcataatac aatgaataac tttgttaaaa gacatctggg tccgcgggaa
    14521 tcagaaatac ccgaaatgct taaggtagtt ggtgtaaaat cgatggaaga acttattgaa
    14581 caggtaattc caaaatctat caggctgccc gaagaaattt cacttcccga accggtaagt
    14641 gaatatgaat ttttaaaagt gattcaggaa attgcatcta aaaaccgcat ttgcaggagt
    14701 ttcataggaa ggggttatta tggttgcgat atgccttctg ttatcaagcg aaatgttttc
    14761 gaaaatccgg gttggtacac aagctataca ccttatcagg ctgaaatatc acaaggcagg
    14821 ctcgaggctt tactgaattt tcagactctc atcacagaac tcacaggaat gcagatttcg
    14881 aactgctcat tgctcgacga agcaacgtca acagctgaag ctatgagtat gatgctggag
    14941 cttcgctcaa ggcaggcttc taaagaaggc aggaatgttt tgtttgtcga taaaagttta
    15001 ttccctcaaa cgattgatac gctaaagcta cgctcggaac ccataggtgt tgaattggta
    15061 attggtgatt ttgaaaccgt tcagttcagc gacaaaatgt ttggggctat tgttcagtac
    15121 ccttctgaaa caggtgaaat ttgcgactac agtgctttta ccgaaaaagc gcaccagcat
    15181 gagatcttgg ttactgctgt tgtcgattta atgtcgatgg tgttactggc acctccaagt
    15241 gaatgggggg ctgatattgt taccggttgt acacagcgtt ttgggctacc catgtatttc
    15301 ggagggcctc atgccggatt tctggcgaca agagacgatt acaaaagaaa aatgccggga
    15361 agaattattg gcgtttctat tgataaattt ggaaaccgcg cattaagaat ggcactgcaa
    15421 acccgcgagc aacacattaa acgcgaaaaa gccacttcga atatatgcac cgcacaggca
    15481 ttgctggcaa ccatgtcggg aatgtacgcc atttatcatg gcccggaagg cttaaaagaa
    15541 atagccggcc gcattcattc tgctgcatca attattgcaa acggcttgaa taacttaggg
    15601 tacaaggtaa acaacaagaa cttcttcgac actctacata ttacccttcc tgaaggcatt
    15661 aaatcgtgcg acattcaggt tcttgccaat gacaagaaca taaacctgtt ctacattaac
    15721 gaaaagacat tgtcaattag cactgatgag gtagtaacgg tggatgaggt aaatacatta
    15781 ctgcacatat ttgcaaaagc aaacaacaaa ccttttacag cagtaacatg catcgaaaac
    15841 aaaaaagctt tcgagttaaa attttcccga aagagtgatt ttctacaaca gcaagtattc
    15901 aacagctatc attccgaaac tgaaatgatg cggtatatta aaaagctgga gcgaagagat
    15961 attgccctta atcacagcat gatacctctg ggttcctgta caatgaaact caatcctgca
    16021 acctcgatgc ttccgttgag ctgggacagt ttttgtaaca tccacccatt tgctcccgcc
    16081 gaccagtgga aagggtacaa gcaaatgatt gaagaacttg aaaaagatct ggctgccata
    16141 accggattcg caagcatcag cctgatgccc aattcgggtg ctgccggaga gcataccgga
    16201 cttatggtta tcagggaata tcataagaac aacaatcagg cttatcgtaa catttgttta
    16261 ataccagcct cagcacatgg cacaaaccct gccagtgctg taatggcagg aatggaagtt
    16321 gttgtggtta actgcgacga taaagggaat atcgacgtgg atgatcttag cctgaaagca
    16381 gagcaacata aagacaagct tgctgcaata atgattactt atccttccac acatggtgtt
    16441 tttgagagtc gtattctgga aatgattgac atcgtgcata aaaacggagg tcaggtatat
    16501 atggatggtg ccaatatgaa cgctcaggtt ggacttacga accctggatt tattggtgct
    16561 gacgtttgtc atctgaattt gcataaaacc tttgctattc cccatggcgg tggtggtccc
    16621 ggagttgggc cgattggcgt tgccagtcac ctggtcgatt ttctcccaaa ccatgttatg
    16681 atgaaaactg ggggagcaaa aggtatccat gcagttgcat cggcaccata cggcagtgcc
    16741 ggagtgcttg caatcacata cgcctatatc aggatgatgg gtacagctgg tttaaagaaa
    16801 gctactgaaa tggcaatttt aaatgcgaat tacattgcat catcattaac gggatactac
    16861 aaggtccttt tcaccggtga aaccggcaga tgtgctcatg aaatgatact cgattgccac
    16921 aatttcaaag aaagatatgg tgttgaagct gctgacatcg ctcacaggct catggattac
    16981 ggtttccatg cacccaccct atcgtttccg gtacacgaaa ctttgatggt tgaacccact
    17041 gaaagcgaaa gcaaacagga gctcgaccgt tttattgaaa ccatgaaatc gattctggcc
    17101 gaactagaag atatttatac tgggaaagcc gataaaattg acaatcctgt taagaattca
    17161 ccgcataccc aggttgaatg tacctcagat gaatggaatc acccttatag tagacagcaa
    17221 gctgcattcc cattggaatg gattaaagag aacaaattct ggccatatgt tgcaaaaatc
    17281 gataatggtt tcggcgatag aaacctggtt tgtacctgcg aaccaataga gacctatatg
    17341 aaatagcaaa gactataaga tatattaaat gaaaaatcct gcgtttttat gcaggatttt
    17401 tcatatcagg gtttcatcca tctgaattgc tccgctttat ttccataatc tccagctctc
    17461 tgatagtggc attgtcaatc tggaatcgca acagtgtttt cactttgtgg atacctctgt
    17521 tcccagctgc accaggatta atgtgcaaca acgaaagttt tttatcgggc attattttta
    17581 atatatgcga atggccgcta ataaacaagc gtggcgggtt taaaaatatt tccggtttca
    17641 cacggggatc atatttgccg gggtacccgc ctatatgtgt aatccacaca ctcagtcctt
    17701 ctgcaacaaa acgtaaactt ccagggtact caatacgcac atcatagccg tcgcaattcc
    17761 caaaaactcc cttgaatggc ttaaaacttt tcaaagtatc ggcaacagaa acatttccca
    17821 tatcgccggc atgccatatt tcgtcgacat cgctgaaaaa ttcgtaaatc gccggttcta
    17881 catacccgtg cgtatcgctc aataatccga ttttcagcat aggtcaaagc attttaacgc
    17941 taaagtaaat atattataac aaaatacaag tcattgagaa atgcgatagt tacaaaaatt
    18001 cagtattttt actcattaat ataaacccac taagttatga gcatgtcatt aattatatta
    18061 ctgatcgttt tgatttcgtt gatggtggca gttttagtgc tgacaatagt attccggcct
    18121 tcacaacaca atgagaatgt tgccttcgac aattatttta aagatttgca aaaacaaatc
    18181 aaccagtttg aagccggatt gaaaaatgaa tttcgtatca accgcgaaga aagtgcagcc
    18241 atatcaaagg aaaaccgtac agaacttagc aatacactaa aagatttcag gcaggaactt
    18301 tccgtaataa caaaaaacat cgccgatcaa accaccaata acaacaaatt gttaagagac
    18361 gaaatcgcac ttgttttcaa agatttcaac gagaacttca ctaagaacat acagtcgatg
    18421 aatgacatgc aacgcgaaaa gttcagtcaa cttgacgaaa gacaggttaa gctaatagaa
    18481 agcaccgaaa aacgcctgga tcaaatgcgg gaaaccgttg acgaaaaact tcagaaaact
    18541 ttgaatgaga ggttggggca atcattcgaa acggtaggaa ggcagttaaa ggaggttcag
    18601 gaaggactgg gcgaaatgag aactctggct caggatgtag gaggcctaaa aaaagttctc
    18661 agtaatgtaa aagaccgtgg cggcctgggc gaattgcagc tcgaaatgct gcttgagcaa
    18721 attcttgcgc ctgaacagta tgcagccaat gttaaaaccc gtcacgacag ttccgaaatt
    18781 gtagaatttg ctattaaact tccggggcgt gatgatcaaa aatcgcatgt atggctacct
    18841 gttgatgcca aatttcctaa agatgcttat gagcaattgc ttgtggcata cgatactgcc
    18901 gacaattcaa ttattgaaac cgcaggaaaa aatattgaag ccacaattaa aaaaatggcg
    18961 aaagacattc acgacaaata catcgatcca ccaaatacta ccgattttgc cataatgttt
    19021 ctgcctttcg aaggtttata tgctgaaata atccgtcgtt caagccttat ggagcaactc
    19081 cagcgtgaat ttaaagtagt tgttaccgga cctaccactt tagcagctat tctgaacagc
    19141 cttcaaatgg gtttccgtac acttgcactt cagaaacgca gcagtgaggt atggacaata
    19201 ttgggtgctg tgaaaactga atttgaaaaa ttcggcgact tactttcgaa agcccaaaaa
    19261 caaatagcaa ctgccgggga gacaatcgac cagttagcag gaacacgtac gcgggcaatt
    19321 acgcgaaagt tgaaagaagt tcaggcactc cctgcaggag aagcagagca tgttttacca
    19381 gaaattgctc aggacgaaat aattgatgaa gaagaaacaa tttcgtaaag tcagctacaa
    19441 cacgtatcaa aatgcaatat tttattagta atatcaaggc attcgaagac atcggaattc
    19501 ttaataaaac cgaatcgcaa cactgcatca gggtgctgcg tcacaagcct ggtgacaaaa
    19561 ttcttgtaac caatggtgat ggattactat tagaagccac cattctcacc gctgacccag
    19621 gtaaatgctc ttttcatgta gtaagacaat tagaaagccc aaaacaacat ccgtattatt
    19681 tacacattgc cattgcacca acaaaaaata ctgacaggtt ggagtggttt gtcgaaaaat
    19741 cagttgaaat cggtattaac gaaatttcat ttgtttcgtg ccgtttttct gaacgaagag
    19801 cggttaacat agaacgtatg ctgaaaattg cagagtctgc aatgaaacaa tcgttaaaaa
    19861 cacgacttcc tgtaattaac ggaatcgtgc cttttactga ttttatcagt aagagatcga
    19921 ttggattgaa gtgtattgcg cactgtatgg gtgataaaaa agtgcactta accgaaagtt
    19981 tgaacagcgg acataaccat atcactgtaa taataggccc tgaaggagat ttctctgtgg
    20041 aagaagtgga agctgcaata aaacaaaact attatccggt cagtcttgga gaaaatcgtt
    20101 tacgcacaga aaccgccggt ctggtagcct gtgtaataat tgcctcgaaa ctaaattaaa
    20161 acattaccag attaagctgc aggccaactg aaaagggcgt taccacaact tcttcatttg
    20221 caaatacatc ggccatatta taacgcgcaa atatggcgaa ctgattgtag cccattcgca
    20281 aaaaaacacc ataattctgg ttttctagtt caacatctct tttagaacga agtttaattc
    20341 tttcaagctt agtgttgttt tttttaacaa aaccccatcc ggcaacataa ctgtagtaag
    20401 cccctccatc gatataccat ccgaaatcat gatcacggtt ttgcttgaaa taaaaacggg
    20461 tgtaaaaagc gccggtaata ttgttctgat aggttctcac cttatcgaat gtgctcactt
    20521 cgggcacaat tgagcgtgta ttgggaaggt ttttcattct atgaaactca ttctcatacg
    20581 aaagatcaac gcctatatcc cagatactca ccacctttag tttgtaagaa ataccaattg
    20641 cccagttacc gcttcccagg ggcatttctc caacaggttg ctgtaatggc actgccatat
    20701 tatacctaac aaaataatag ctgtaatgcc tcatttcagg cccccactca gtgccattgt
    20761 agtaactttc gccatcattt ttatcgtaaa caacagcctg agcctgagca cagagtgtaa
    20821 caagcgaaaa tatcagaata gtacaaagtt ttctcatagc tgcatgagtt tattgggtaa
    20881 aaatacaaat ttgttaatga ctaaactcat taatttatca acataacatt tgtaaagaaa
    20941 tgtccggcta aaatcagcga cttatattgg tttaacccaa ataggcttta agaagtttac
    21001 ttttagaatt gtggcgtaag cgcctgatgg ccttttcttt gatttgccta acacgctctc
    21061 ttgtgagacc aaactcttcc ccaatttctt cgagggtttt ctcttggcaa ccaattccaa
    21121 aaaaatacct gattatgtct ctttcccgct cagtaagtgt ggcaagcgtc cgatcaattt
    21181 ccctgctcaa cgactcgttg attaaggttg aatcggtatg tggtgaatct gaattttcga
    21241 gtacatcgag caaactgttt tcttcgcctg aaacaaatgg tgcatcaacc gaaacatggc
    21301 gtccagaaac acggatggta tccgcaactt tttcaaccgg aagatccaat tcctttgcaa
    21361 gttcctcaga ggttggtcgt ctttcgtttt cctgctcaaa tttgctaaaa gctttattaa
    21421 ttttatttaa agaccctacc tgattcagag gtagccgaac aatacgtgat tgctcggcaa
    21481 gagcctgtaa tattgattgc cttatccacc atacagcata cgaaataaac ttaaagcctc
    21541 tggtttcatc aaatttttct gcagctttaa ttagtcccag gttgccttca tttattaaat
    21601 cgggtaaact taagccctga ttttgatatt gctttgcaac cgaaaccaca aatctaagat
    21661 tggcccgggt aagtttctca agtgcagccc tgtcgttttt cttgatgcgc tgtgccaatt
    21721 caacttcttc ttctaccgaa attaattctt ctttgccaat ttcctgcaaa tacttgtcaa
    21781 gcgaagcgct ctcgcggttg gtgatcgatt ttgtaatctt taattgtctc attcttcctt
    21841 tcctcgataa taattctgtg ttctaaatca gtaaaatcta tttaattacc tcaccaatac
    21901 gaaaatgaag gtaaaaaggt tatgtaaatt tcaatttttt tacatttttt tttcaaacaa
    21961 actacaaacc attcggccac atatatctac aagctttgat atgactgaat tcctgaatgt
    22021 tgatcactta actacaatta acttcgactt atactgtgag tcttcggttt gaagcctgac
    22081 aagataacat cccggctgaa acatgtttgt attcagagta gtgactgcgt tgaataaggc
    22141 atcgcgataa acgagttttc catcaatagt tgaaatatca agattacccg ttatatttcc
    22201 ggcctctatt cttacaacac ctgaagcagg gtttgggaaa atcttgattt cattttgaaa
    22261 ctgcataatg ttttcaccgg taacatctcc cggcaagtaa acaggtgccg accataagta
    22321 atctccatct gtttgaacag ccctgacaaa aacccattga tcgttgccaa gagatatgtc
    22381 gataacagca ttaaaatcac ccgaaaaatt ggtaatacta tgaataatcc cattctgact
    22441 aaccacttca atgcttgcat aattttcaac atcagtatcc gttaaattaa tgtgcagcga
    22501 catcgtatga tccactggca aactcccgcc cataggcaca ccgttaatat caatccaaac
    22561 agatgcattt ttatccatag ttgcgaaagt acggccagct atgattgctt cgaaaagctc
    22621 agcgcgggta agagtatttg cccatacacc ggctctgcca ttgtttttgt ttccccagtc
    22681 tgcagaatga ttgtcctgat tccaaaccgg cgataaatgc catccttttg aaagcgcaaa
    22741 ttcgtaccag tcagtcgctt gttgaacatt ctgaaactca atcaacgcaa agatttcgtc
    22801 tgtcgattct ccaacatatt caaaatcatt ccaattatta aaagtttcct catcacccgg
    22861 gtgattgaat tgcgcaattg agggcgggtt ttccgataac cagtccagaa actcgtcaaa
    22921 atcgttataa taccacagat tgagcacatc cgaaataatt tcatcggtgt taaaaacatt
    22981 gatatgacca tacaaaggag agccccattc ccatgcagca atagcaacga agctcccatt
    23041 ttcggtggca tcgtcagcat catttttaac attgtaccat tccaacatat caatctgctc
    23101 catatgatcg gttactgcca gaaagtcgat accggcagta tatcgggcat attcaaatgc
    23161 atcttccggt tctccctctc catcggaatt tgaagtatgg gaatgcaggt taccaaaaaa
    23221 tgcagtatac gtttcctgag cattcattat agtgggaacc agcattaaag agcacataaa
    23281 aagcaagtaa cttttggtca tagtttttgg gtttaagtac aaatataggc tttttaagca
    23341 aagattacaa ttctcaaatt aatttaaaaa taagaaaagc taatccacta aaagaatata
    23401 gaactacaaa ccaaaagcat aatatgccac agtgccattg ggatagatgc cttcgataaa
    23461 aacagcgcca tcggagtttt tgagtgcgtc ttcaacgtca tcaaccgaat cgatgtttcg
    23521 gttgtttatt cgtgtaatga tatatcctgg ttgcaccccc ctactcagga gctttcccga
    23581 cgacaatgac tttactttaa ctccgttacg aattctcaat ttctgcttgt cggcatcggg
    23641 aacaggttcg aagttggccc ccaaaatttc gacgcttctg gcttttacaa tatctgttgt
    23701 tccatactta ttagtaagca tggcatttac tgtatatggc agtccattgc gaataattct
    23761 aatttccacc ctgtcgccgg gcctgaattt actcagctgc tcgagcattt cagctgtgga
    23821 gtttactttg tggttgtcga cttcaagtac aatatcgccc tttttcagat cgcttttcga
    23881 atcagagcca tcgctccaca cacctgcaac atatattcca ataggccttt ccattttcag
    23941 gcttttagca agatcagcat caatatcagc aaactcagcc ccaaggtaag cacgttgcac
    24001 atcaccaaat tcaatgaaat cggcaactac ttttcggacg atattcgaag gaatggcaaa
    24061 tgagtaccca acaaaagaac ctgtgcgcga tgctatggcg gagttaatac cgattagttc
    24121 tccattcagg tttaccagtg caccaccact gtttccgggg tttatcgctg cgtcggtctg
    24181 aataaaggat tcaatatcca tgctttgccc catgatattt atattccggg cttttgcact
    24241 gataatacct gctgtaaccg ttgaggtcag gttaaaggga tttccaacag ccagtaccca
    24301 ttcaccaact ttaacatcct ctgaattggc ataaggaata aatgggagat cgtttgcttc
    24361 aacctttaat aaggcaaggt cgctggctcc gtccttgcca atgagttttg catcaaacaa
    24421 acgtttatca ttcaaaacaa cttgtataac ctctgcgttt tcaataacat gattgttggt
    24481 aataatgtac ccatcggaag aaagaatcac tcctgaaccc gatgccataa caggggtgga
    24541 ataccgaggc gcagtaccga aaaaataatc gtacaatgta taattgggcg cattgtactg
    24601 agttttaaca tgtactactg cgttcacggc cttctctgcg gcatcggtaa aatcaggagc
    24661 gctaaactca ctctgactaa aaagccctgt tttttgaacc ggtacatttt tgtggctgta
    24721 atcatattgc tgcacaagcg ggtgtttctc attactctgg aatgaaccca aactgtcggt
    24781 gattaatgct gcagtaagaa ccactattcc tcccagcatt ccgccggcca gacccagtaa
    24841 aaactttttc ttttccataa tacctatttt ttataagtaa caaattttgc gtacatttac
    24901 gatgttttaa cgatttttgt ttcctttttc ttgagcaaat tttatgtttt aacatttttt
    24961 tatggatatc agtttttcga aataccacgg ggcaggaaac gattttgtaa taatcgacac
    25021 acgaaaatcg tcattaaatt taaatagcaa ccagataaaa caactgtgtc atcgaaggtt
    25081 tggaattgga gccgacggtt taatgctttt acagaattcg gcgcaggaaa cttttaagat
    25141 gaaatattat aattctgatg gcatcgaagg tactatgtgt gggaacggcg ggaggtgcat
    25201 tgttgcattt gcatcagatt tgggtttgtt aggtacagga acaaaaacaa gctttgaagc
    25261 tatcgatggt gttcaccacg cacagaaact ggggcataat aatatttctc tgtgcatgaa
    25321 cagcactcaa atacctgtga gatttgaaga cggttttttt gtggacaccg gttctcctca
    25381 tttcgtaaaa tttgtgcccg atatcagcca aatcgatgca gaaacggaag gccggctact
    25441 tcgtttcgaa aaacgctttg ctcctaaagg gaccaatgtt aacttcgtac aaattctatt
    25501 gacaggactt aggattgtta cctttgaaag aggcgttgag gcaataactc ttgcttgtgg
    25561 aaccggtgca gtggcagctg cggtttgttt tgctgagctt gaaaataaaa actctcttca
    25621 aatacctgta atatcagctg gaggcgaatt gtttgttaca ctcgaaaaaa agcctgatgg
    25681 gtatcacaac attgttctct caggtccggc catgcgggta tttaccggca cattctcact
    25741 ggtgtcagaa tagtatgggg taattggcac aaaacttgaa atcaaaacct aaattaatat
    25801 tatgaaaccc acattcctat ttgctatttt ttcatgctgc attttaatag ttgcatgcaa
    25861 atcacagtcg ggcacttcgg aaaacaataa acctgacagt aacgccagca acgtaaccca
    25921 tcctattcag gggaataccg actctcaggt tgcgaccacc gatatcgaaa aacccaagcc
    25981 ggaaggctat gacaaaaata tttcgttacc acaaaatcta acgaactttt cgtttgattt
    26041 ttacaaattt ctcagcgagg agagtaaagg caaaaaccta tgtttttcgc ctgtatcgat
    26101 gaatatagca cttgccatgg tgtatgaggg tgcgcgcaac aatacacttg agcaaatgag
    26161 ccaaaccatg caccttccgg ataaccaaga gctgttcagt aacgaatttg cttcatacct
    26221 tcggagcatc gaacttctgg cgaaagattc tgctgttgaa ttcaatcttg ccaaccgcgt
    26281 gtttgtggaa tcgagcttcc caatcctcga atcatatcga agtgctataa aacaagcgtt
    26341 caatggggct tttgagcaga tggactttat taacaagttt gctgaatcgg agcaatacat
    26401 caacaaatgg gtggaaaaaa tgacaaaaga caggattaag gagcttttac ctgcgggtac
    26461 actgagtagt gaaacaaaat tggttcttgt taatgccatt tacatcaaag cccgatggaa
    26521 atatattttc gatgaaaaag ctaccatgaa gaaagatttt cacatcaatg gcagcaatat
    26581 agttcaaacc gacttcatga tcagaaagca aaaaggaatc agatattaca cgaataacga
    26641 catctgtgct atagagcttc catatacctg tgacggaatt tcactaatta taataaagcc
    26701 ccacaaaggt agcagcgaga atttatcgtc gtttattcct acagggagcg attatgaaaa
    26761 aatgttatcg gatatgaagt atcacactgt tcatatggaa attccaaaat ttaaaacaga
    26821 aacttcgttt tcgctgagcg atccgttagt tgcaaaaggt atgtccgatg ccttcagtcc
    26881 gcgtgccgat ttctcgggta taaccggaaa taacgacctt tcggtgagcg aagtgctgca
    26941 aaaagtattc tttgaggttg atgaaaaagg ttctgaggct gccgccgcct ctgctgttgt
    27001 gatgagagca acatcagccg ctccaaatca cgaaatggag gaaataatgt attttattgc
    27061 caatcagcca tttatttata ttcttaagga aaacaacagc gatacgcctt tatttattgg
    27121 ccaatatatt tcgccacaat aatttattac acaaaattaa aatggcagga ataagtcctg
    27181 ccattttttt cattcggtat taatcaaccg gcgtatattc ttcaagtacc tgatcaacgg
    27241 tagggggggt taccgcatta ttggtaacag tgtattttaa aacaatttta ctgatatctg
    27301 atccgatata ttcaattctc gactgcggtg taaaaccatc gctttcagtt ttcccggtaa
    27361 taaaaacagc tgcgtttcct attgaatcgg tgtaagtatt cgtttgaata tttatgtatt
    27421 cgaatttgcg aagagcattt gcttcgtcat atcccatacc tctgaaccac ggtacagaat
    27481 tattgtcgtc agataaa
//
LOCUS       DAOOQP010000026        27138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2110_length_27138_cov_6.229369, whole genome shotgun sequence.
ACCESSION   DAOOQP010000026 DAOOQP010000000
VERSION     DAOOQP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27138
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_27138_cov_6.229369"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3057
                     /locus_tag="PLK75_04530"
     CDS             <1..3057
                     /locus_tag="PLK75_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38545.1"
                     /translation="DYQGNLGYGDQPPAIGIDFFEGPYQDNDGIDNPTSFDTLNGVKV
                     LNCQKGDILNGNINGLNFGDGTVDNERWGMRRFLYFNNASGNPATQDPNSAIEHYNYL
                     TGFWKDNSPLCYGGTGHTSGGGDASVPTDFMFPGKPTTDICGWGQGGVIMPDWSEETE
                     GNPAGDRRFVQSAGPFILKPGAVNDITIGAVYARAASGGPWASVEAVRKADDKAQILF
                     ENCFRVLDGPDAPEIEIIEMDKQLIFHISNKTNSNNYLEEYIEKDPSIVCTGEYEPCD
                     EYYRFQGYQIFQLKNESVSISDRYNNNLVRQVFQCDLKDEIEQIVNYTWSDELGANVP
                     VEEVNGLNEGITHTFVINTDNFATGDNRLVNHREYYYTAIAYGYNNSMLYNQQVQETF
                     NGQKKPYLAGRNNIKRYKAVPHINDPEDGGTIINGEYGYGPEITQIEGHGNAFNVLDL
                     KQESIDEIMSGHPWKAENPVYENGKGPVTVKIIDPLNVPEDSYTLQLVDAAIWSYGWI
                     KEGATWYIVNSAGDTVKSDVSLNFIGNEQLIPDWGLSVNVVQTEYAGNRNKVKNNGFL
                     EASYEFEDETKPWLYFLPDGDGNDEYNWIRVGTYQNKEGELEAGGADKSYDDYIGYDP
                     DENYEKILQGTWAPFKFASDFKFGPAYKAARSIQNIEKYEPISSVDIIITSDTSKWTR
                     ACVVEMCENEWTVDEYNHPEQVTPWVNYLSEGNALRFTLRKQGSVDKNGQALNDGTHG
                     LGWFPGYAIDVRTGERLNIAFGEDSWLVGENGRDMIWNPSEGIASNTGPLFGGKHYIY
                     VFGHNQNFTNPQLLAPRYDSCAFIYSKLLNYETSNNALDLRAAWTSCMWTAIPLKNPS
                     FDLLSSDVKIRLRVVTPLHKALGNFAVEEPVNDNLPMYTFSTYDVKTINNDTVQAVSA
                     LDLINIVPNPYYGHSYYETNQLENYVRITNLPRKCTISIYNVGGTLVRRFEKDSDITY
                     IDWDLKNTYNISIASGLYIVHFNVPGVGEKVVRWFGALRPLDLNNF"
     gene            3090..4202
                     /locus_tag="PLK75_04535"
     CDS             3090..4202
                     /locus_tag="PLK75_04535"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOY38546.1"
                     /translation="MKKCFSQIVSVMIAGLLLLPALSFGGNEQRAGSSGASELLINPW
                     ARSSGWGDANVACVMGLEGMYQNIAGIAFTRKTELIFNNTQWMVGSGVVINNFGFAQK
                     VGETGVLSVGVMNMNFGEIEITTPDLPEGGIGTFKPSYTNIELAFAKEFSHSIYGGIG
                     VKIINEGVANATASGFAIDAGIQYVTGFGKDKAGNRKRDNLRFGIAMKNVGSTMKFKG
                     DGMAFTGIVPNGTSMTVEHRSQEFELPSLIKIGLSYSINLDAKVDSVSETVISNHHLT
                     IAANFTANSFTKDQFHFGMEYGFKNIFFLRGGYMYEKGITDVNDRTTALTGPTAGVSV
                     QIPMNKEKKSVIAIDYSYRTTQPFSGIHTFGARVTL"
     gene            4411..4884
                     /gene="greA"
                     /locus_tag="PLK75_04540"
     CDS             4411..4884
                     /gene="greA"
                     /locus_tag="PLK75_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOY38547.1"
                     /translation="MSKITYMTEEGLKKLKDELDRLTSVERPSISKQIAEARDKGDLS
                     ENAEYDAAKEAQGMLEMKIARLQETIINARVIDPAKIDNKKVQIMNRVKIKNLKTNAI
                     LEYMLVSENEADLKEKKISVNSPIAKGLIGKKQGDVVEIHVPAGVLNFEIIEISM"
     gene            4897..5292
                     /locus_tag="PLK75_04545"
     CDS             4897..5292
                     /locus_tag="PLK75_04545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOY38548.1"
                     /translation="MGSIFSKIINGEIPCHRIAENDKFIAFLDIQPLKSGHTLIVPKK
                     ETDYIFDVTDSDLADMIVFAKKIAIAIKKAVPCKRIGVAVIGLEVPHAHIHLVPLDAE
                     SDLRFSNTRLKLSQEQLSVIAEKITSFLD"
     gene            5405..5881
                     /gene="rnhA"
                     /locus_tag="PLK75_04550"
     CDS             5405..5881
                     /gene="rnhA"
                     /locus_tag="PLK75_04550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOY38549.1"
                     /translation="MTENLKIIIYADGAASGNPGPGGYGAILMSGRHKKEISRAFRMT
                     TNNRMELLAVIDALSLIKKVGADVTIFTDSRYVVDSIEKKWLNSWVATGFRKKKNADL
                     WQQYLDVAARHNIVLHWVKGHAGNELNNRCDELAVNAYRSGVFEIDEVYEQQRNEI"
     gene            5856..6326
                     /locus_tag="PLK75_04555"
     CDS             5856..6326
                     /locus_tag="PLK75_04555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY38550.1"
                     /translation="MSSKETKYNTELRAVRFSYDNDELLRIALTIRKLVFVDEQKIDP
                     DLEFDGYDKDAQHFLLYFKSEPVATARWRETDAGIKLERFAVMKQFRNHGYGSKLMAF
                     IMQQNLTGKHLYFHSQLSAVGFYEALGFEKDGELFYEAGIPHYAMKKATKPNLQ"
     gene            6323..7807
                     /locus_tag="PLK75_04560"
     CDS             6323..7807
                     /locus_tag="PLK75_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY38551.1"
                     /translation="MTANPIKRLAGQTAVYGLSSIIGRLLNYLLVPIYTRLFIPEVYG
                     VVTELYAYVSFLLILLTYGTETGYFRFAQDKERFATTYSTLLSSLAVSSLAFIGLISI
                     FIHPIASAMQYTAHPEYIIWMAVIVAIDAFISIPFARLRLEGKAMKFASLKLINIAIN
                     IAMNLYFLLLCPYIASKNPESLFLIIWSPGIGVGYVFISNLIANITTLLMFIPSFIKV
                     KYRFDVQLLKQLLLYSFPLLIAGFAGMINETLDRILLKYCLPDQVNAMEQIGIYGANY
                     KLAILMTLFVQMFRYAADPFFFAEAKKSDAKATYALVMKYFVIGGLFLFLGVMLYLDF
                     VKYFIDPEYHSGLMVVPVLLMANLFLGMYYNMSIWYKLNDKTIYGAYIAIIGAFITIA
                     LNVALIPFFGFIGSAWATFICYLTMFIISYLLGQKHYPVKYPVKAILLYFVVALVIYF
                     ISIVIPPTTVFLHYAFNTVLFVAFGIFVVIREGLYKKYILPKLR"
     gene            7864..8307
                     /gene="dut"
                     /locus_tag="PLK75_04565"
     CDS             7864..8307
                     /gene="dut"
                     /locus_tag="PLK75_04565"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOY38552.1"
                     /translation="MGKVKVKIINKSKHQLPTYATALSAGIDLRANLNERVVLKPLER
                     KLIPTGLFIELPESYEAQIRPRSGLAIKKGITVLNTPGTIDADYRGEVMVILVNLSSE
                     EFVVEDGERICQMVVARHATVEWEQSDILNQTERGDGGFGHTGKK"
     gene            8341..9351
                     /locus_tag="PLK75_04570"
     CDS             8341..9351
                     /locus_tag="PLK75_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOY38553.1"
                     /translation="MKIIIPMAGLGTRMRPHTLTVKKPLIPIAGKTIVQRLVEEIASV
                     INQPVEEIAYVIGRFGEEVESELRSIASKIGAKASIYYQDIAMGTGHAVWCASPSLNG
                     PLVVAFADTLFYAGFKFDASTDGTIWVQKVKTPESFGVVTLDNNNIINGFVEKPKSFV
                     SDLAIIGIYYFNDGLNLQKELQRLIDENIKGNNEYQLTDALENMRRNGMKFIPGEVDE
                     WLDCGNKDATVLTNQRILAHEKESLTQVENNNIINSVIIQPCYLGHDVKIENSVIGPY
                     VSVGRNTVISNCIIKNSIIQNNSQLNGAFLHNSMIGNDVIIDKRPDDLSVGDFNQIRK
                     NS"
     gene            9348..11075
                     /locus_tag="PLK75_04575"
     CDS             9348..11075
                     /locus_tag="PLK75_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38554.1"
                     /translation="MNKNLTAYIVLVITALLLYSCKTMEKSPVAKGKDNTSYFAFKSE
                     LLDGVRNKMTGNYDAALDNFSRASRWDPKQAVSYYEMATVLAIKNDYPAALEYCKLAT
                     GLSKNNYYYTLLLASLYQANAMSKEAAETYDEVIRLKPSIPDNYFSQSSIYLSVSDPK
                     KALQSLDKAEEYFGVTHEINSEKYDIAVTSRNYPVALKEINKLIEAYPKESIYYAMLA
                     ELYKVMGKTANVAEVYQMLEGMDVRNGMVLVSLAEYYSSQGNNEKAFAILEKAFALDD
                     FDMDSKIQILFNLIVPTENDVMLTEKVYRLLEILVLKYPDEPKVHTIYGDYLVRDRRW
                     EEAQSRFNFVLKNEKSHYEIWEQALLIDNQIKDWTSMYDRSNEALQYFPMQVTLYVFN
                     TEAAMQLSKYSEAVMAAESGLRFAAGNDDIKLDFYIYLGEAYHKTNQHELSDSAFENA
                     LKLDSQNTFVLNNYSYYLSLRNERVDKALQLSENLMLLESENANFIDTHAWALFRNQK
                     YNDALIYIEKAVIIDGEKNPNILEHYGDILSKTGKTEKAVQCWKKAKEMGSGSKTIDL
                     KIEKQQYTE"
     gene            11072..11977
                     /locus_tag="PLK75_04580"
     CDS             11072..11977
                     /locus_tag="PLK75_04580"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOY38555.1"
                     /translation="MRGKTLVKLVLLIVPLFLGVGCSKVFYTGGIKNNYKPVKLFQKI
                     SESEIQYHTFMLKADCEFEVNGQSQSFQLNARIQKDSVVWLSMKMLGVEGMRVQCLKD
                     SLYIVDRINNQWSVAAYKDLNNVFSMELCYQFIQAVITNTFFFYPPTSDTLKTLHDFD
                     NCKDTLNYCMSSGGWKRYQRYISDSTGSRRNENRPGRYNPERYHDNEQEAGEASIFQV
                     IKVYPEIFKIAYVLIDNYMNGQTMEISYEEPRLFGTNFFPGKIRIWLDSPETEIILNI
                     ETERIEVDIDQRYPFKIPSNYERMK"
     gene            12008..13216
                     /locus_tag="PLK75_04585"
     CDS             12008..13216
                     /locus_tag="PLK75_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOY38556.1"
                     /translation="MIKRIPIAKAGIFLMLFCLMASVTFSQSRKELENKKKKVLQDID
                     YTNKLLKENEAKKVDSYNNLLLINKKVESRQELILSIGEELAIIDEHIDDNKMVLDLM
                     QEDLKRLKDEYAEMIYFAYKNRNTYSSIMFILSSEDFNQAYRRLMYLQQYSDYRKRQV
                     LAIQSTQLMIENQIAKLDALKAEKQALLGEKNAEKEQLKEEQQQQSKTLNKYKNKEAE
                     LKKQLKKQQESAAQLQREIEKMIAEEVNKSSGGKSNTYVLTPSEKLVSTNFANNKGKL
                     PWPVERGVIVSSFGKHAHPVLENVTIDNAGIDISTTEGAVVRAVFEGEVRRVFTLPGA
                     QNAVIIRHGNYLSVYTHIAKVYVNTGDKVTTKQAIGSVYTDKEENKTIVHLEIWYESS
                     KQNPASWLAK"
     gene            13223..16522
                     /locus_tag="PLK75_04590"
     CDS             13223..16522
                     /locus_tag="PLK75_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38557.1"
                     /translation="MEKLVSDIILKIEGFIKKYYTNSIIKGLILAFSISGLYYLIIVL
                     AEYFGHFTVAIRTLAFYTGIILFSIVWIYYIIIPLIKLIGIGKRISYKQASVILGTHF
                     PEIGDKLQNTLELAELIKHNPDASDLILASIAQRKMLISPIPFRNAVQYRKNVTYLKY
                     LLPVAAVFVVLLILWPSIISEGTQRIVRHSQEFVLPPPFSLNIDSDTLLVKKGTDFNV
                     KVQVEGNVVPAEVYIHIGSNRYVMKKDNPREFSYVIRNLNNNILIHASSGDIISGTVE
                     LKVLPSPAILNFSLNVVPPAYTGIEQKLYTNTGDVSVPCGSELIWQFGTANINTMNML
                     VNDSIEIAGERNNNTFSFRRKIIQPLKYNMLASNEYFKNQENVAYTINVIPDVYPGIS
                     VKQVQDSVDLNIFFFSGTIDDDYGFNSLSFHYKTDSLHVASLPVGRGVTTQNFYYAFD
                     FSGINIPGNSVTYYFEVGDNDAVHGSKKTRSTSFQFTVPTIEEIESKTEEANKSIASK
                     MDQAKRLANEIQRDINNLQRRMMNENLSEWERNQMMNEILEKQKELEQLANEIVEEQK
                     KSNQYKNTFNDKENLLEKQKQIEELLTQLMDEEMMKLIEELQKLMQEFDQDKFNELAN
                     DLNQNYEELSKQMDQDLELLKRMEVEERMENTIDKMDQLAEDQEQLAEETENKELSND
                     ELLKKQQEQMQRFEDLQKEYENTLEKNNQLKEPFQMDDFSKDFEEISNQMQQSEQNLN
                     ENKNNKASKSQQQSSQQMQSLSAKMQNMMDSNMQQQAAENLDDLRQILDNLISFSFSQ
                     EKLINEINGVGTRSPRYRDIIAEQKKIGDDFNIIRDSLDALAMRMPEVNAIVKKEVNT
                     IFRKLDDIMSGFSELGTQNITTNQQLVMTSANNLALLLEELMKAMQQQMSMQMCGNQN
                     CSKPNNSSQPQMGQLRDLQKGMKQQIQQMIDQMKQGGDSPAQKEQMNKNLAKMLAQQE
                     IMQQMLNEMMTSETLSPESAKILNEINRMMEENIKDMINGNVSEQLLRRQELILTRML
                     EVEKSEHERELDEKRKSNEARDYKISNPDKAFDEKEKQMRFNELLRLSNLKMTQYYKE
                     KYKNYLQNLEK"
     gene            16526..16945
                     /gene="ybeY"
                     /locus_tag="PLK75_04595"
     CDS             16526..16945
                     /gene="ybeY"
                     /locus_tag="PLK75_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOY38558.1"
                     /translation="MAVSFFSEQIAMPALKKRVIKTWVSEIIENHEKKSGNINFIFTT
                     DDMLLEINKKYLNHDYYTDIITFDYSTNRLIHGDIYISLDRVNENASTLNTGTTELFR
                     VMIHGVLHLLGYDDSDNESQEKMRKAEDQCITLLLNK"
     gene            complement(17033..17680)
                     /locus_tag="PLK75_04600"
     CDS             complement(17033..17680)
                     /locus_tag="PLK75_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOY38559.1"
                     /translation="MKNLFLTVVLIIAASSAFSQSDPKAIELLQTVSSKLSAYKSIQV
                     EFRFYVESLQDGSRDQFSGKALYRGDRYRMDLMGQVVFSDGKTNWTYLKDAEEINITD
                     ASETDGTVFNPQNVLNNFSQDYKCRWISDKFENNRTLVEIDLYPVKIEGKKYSKLTLK
                     VDKTKNQIYSIHYVGKDGVSYLVVIDKFVENPVIADKDIIFNSANFPNAEIIDMR"
     gene            complement(17690..20080)
                     /locus_tag="PLK75_04605"
     CDS             complement(17690..20080)
                     /locus_tag="PLK75_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOY38560.1"
                     /translation="MKKESNTKDKTDKKQGKAKKPGMFGSMKNFFTDERFTVSLGILL
                     IAFSILLTLSFTSYLFTWKSDQSILDIPLNKLFSNESLKVDNWVGKIGALLSNLFIRN
                     WFGLASFSFIVIFGVAGFRLLKIRLLPFRKTVFILLIFTLWLSITLSFLLGDKIFFLG
                     GAHGYFMCKWLNSFWGKIGTLTSIGISAFLIIIYSFHSALPAIKRGISKIRNLKKPDL
                     EEANPVFGDIPESEEEDMEEDVSEVTAEEASENVVEFPVETPESETLYDNIELELEEN
                     ENEELISDEDIDIEPMGDYDPTEDLANYKKPPIDLLIDIAASKTEVTAAELNSNKDKI
                     IKTLQDYKIEIAKITATIGPTVTLYEIVPAPGVRISKIKNLEDDIALSLAALGIRIIA
                     PMPGKGTIGIEVPNSNPEIVSMKSVIKSAKFQESTFQLPVAIGKTISNETFVFNLAKT
                     PHLLVAGATGQGKSVGINAIIASLLYKKHPSQIKFVLIDPKMVELSLYSKIERHFLAK
                     LPNEEASIITDVSKVVPVMNSLCIEMENRYKLLTMAGVRTIVEYNDKFISRKLNPLKG
                     HKYLPYIVVVIDEFADIIIQEGKQVEAPISRLAAKARAIGIHLIVATQRPSVDVITGV
                     IKANFPTRIAFKVMSGHDSKTIIDATGANQLIGKGDMLMVETGKAIRIQCAFIDTPEV
                     ESIVDYIGKQQGYSNIYELPDPGMDSGDNGDMSFNDDEKDPLFDEAARLIVRHQQGST
                     SLIQRKMKIGYNRAGRIIDQLEQAGIVGPYEGSKARQVMFQDEYSLEQYLKNSENK"
     gene            complement(20153..20821)
                     /locus_tag="PLK75_04610"
     CDS             complement(20153..20821)
                     /locus_tag="PLK75_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOY38561.1"
                     /translation="MKNLVRIFSLLLFVVLALNVAGQKKAKTFEGSVKYKIDVEGENI
                     DAATRAQMPSEIVVYYKGDNIRTEMITPMFSFISLSNVNDGSVTQMFNGMGMKFYVVQ
                     TKEDLEALKDESEEKPVIKQLDETKVIAGYTCKKAEITSGGQTMEVYYTNELAVPFDK
                     NSQYQMEGIDGIFMEYNMDQNGMIMKFSVKEVSKSKLNQSLFTVPSDYEKKSYEEFKT
                     MFGG"
     gene            21016..21969
                     /gene="arcC"
                     /locus_tag="PLK75_04615"
     CDS             21016..21969
                     /gene="arcC"
                     /locus_tag="PLK75_04615"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOY38562.1"
                     /translation="MNKKLAVVAFGGNALLRAGQKGTIEEQERNVYETCESLLNLIKN
                     DYNLVIGHGNGPQVGNAMLCNIAGNKMFDLPLMPLDICGSYTQGFIGYIIEQQLKNIL
                     LRNNIEKDILTLVTQVVVDKNDTAFQNPTKPVGPYYTKEEMEALVKQNPTETYAADPK
                     GRGYRKVVASPVPIEINNVHSIEMLARSGNIVITVGGGGIPVYYKNDSTIEGIDAVID
                     KDLASALLATKINADEFYILTDVPKVCLNFHTPDEKQLDVVSINEAKQYLEMGHFTEG
                     SMAPKIRAAIRFVENGGNECIITDATQLGVKNAGTRIVRNH"
     gene            21998..23002
                     /locus_tag="PLK75_04620"
     CDS             21998..23002
                     /locus_tag="PLK75_04620"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOY38563.1"
                     /translation="MAFNLRNRNFLKELDFTKEELVFMLNLAKELKSAKYAGTEQQKL
                     KGKNIALIFEKDSTRTRCAFEVAALDQGAHVTYLGPTGSQMGHKETVKDTARVLGRMY
                     DGIEYRGFGQAIVEELAKYAGVPVWNGLTNEWHPTQMMADVLTMIEHSAKPLEQISYA
                     YLGDARYNMGRSLLVIGTILGMDVRIGAPKKLWPDAELIKTCQKIAKESGARVIVTEK
                     PEEAVKGVDFIHTDVWVSMGEPKEVWEERIKLLKPYQVNPAIMKKSGNPNVKFMHCLP
                     AFHNADTKVGKQVSEDFGLKNGVEVTEEVFECKASLVFDQAENRLHTIKAVMVATLGA
                     "
     gene            23134..23304
                     /locus_tag="PLK75_04625"
     CDS             23134..23304
                     /locus_tag="PLK75_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY38564.1"
                     /translation="MAYKISDDCTACGTCIDECPVEAISEGDIYKIDPDVCTECGACA
                     DVCPVEAIHPEN"
     gene            23508..27029
                     /gene="nifJ"
                     /locus_tag="PLK75_04630"
     CDS             23508..27029
                     /gene="nifJ"
                     /locus_tag="PLK75_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOY38565.1"
                     /translation="MAKTKKFITCDGNHAAAHVAYMFSEVACIYPITPSSNMAENVDE
                     WSAAGRKNIFGQTVKVEEMQSEAGAAGAVHGALQAGALTSTFTASQGLLLMIPNMYKM
                     AGELLPAVFHVSARTVAAHALSIFGDHSDVMSTRGTGFAMLATGSVQEIMDIAPVAHL
                     AAIKTRIPFLHFFDGFRTSHEIQKVEYLENDDMEGLIDQPALQLFRDNTLNPEHPVTK
                     GTAQNPDIFFQAKEACNSFYSIVPDVVEYYMKEMTKLTGRKYHPFTYYGAPDAENIVI
                     AMGSVTDTIKEVIDFKLKHGEKFGLITVHLYRPFSEKYFRKVLPKTVKRITVLDRTKE
                     PGANGEPLYLDIKDIFYSDKNAPMIIGGRYGLSSKDTTPAMIMSVLDNMASKQPKSGF
                     TVGIVDDVTKLSLPLKSDVTVLSKGTFEGKFYGLGSDGTVGANKNSIKIIGETTNKYC
                     QAYFAYDSKKSGGFTASHLRFGDKPIRAPYLINSPDFVACHVPAYIYLYDVLKGIKPG
                     GTFLFNSIWSVKETLEKLPDHMKKTLAEKKIKFFMINATEIAERIGLGNRTNTIMQSA
                     FFRISGVIPYELAVKEMKKMVVKSFGKKGESVVKMNHDAIDAGCCNYVEVDIPKEWKK
                     LPNTGFKVIRNNVVPQFISNVADVINSQNGDSLPVSTFVGREDGTFPSGTTKYEKRGV
                     AVNIPEWIPENCIQCNQCSFVCPHAAIRPFLLTEDELKNAPAGTKAIQGIGGTKEYKF
                     RIQVSVLDCTGCGNCADVCPAKTKALTMKPIETQRAEEARWTYMDEVVGYKDHILPKD
                     KTVKNSQFAQPLFEFSGACGGCGETPYIKAITQLFGERMIVANATGCSSIYGGSAPST
                     PYCPHYKSGFGPAWANSLFEDNAEYGFGIATGIDKIREKICLQIENAQWPEKAMNIAL
                     EWKNGKNNAEQSIDASAKFVEILESVNDNTAKEILKYKNYLIKKSVWIFGGDGWAYDI
                     GYGGLDHVIASGKDVNILVLDTEVYSNTGGQASKSTPVGAVAKFAAAGMRTRKKDLGA
                     MAMTYGYVYVAQVAIGSSQNQFFRAIKEAEAYPGPSLIIAYAPCINHGLSLGMGKTQE
                     EQKRAVEAGYWHLYRYNPLLEAEGQNPFALDSKEPDWTKFKDFLKSEVRYSSLLKSLP
                     EEGKHLFDIAEQNAQWRYNYYKRLAGI"
BASE COUNT         8653 a         4970 c         5744 g         7771 t
ORIGIN      
        1 gactatcagg gcaaccttgg ctatggcgat cagccacctg caataggcat cgattttttt
       61 gaaggtccct atcaggataa tgatggaatt gataacccaa ccagctttga tactttaaat
      121 ggggttaaag tacttaattg tcagaaaggc gacatcctga atggaaatat caacgggttg
      181 aattttgggg acggtaccgt tgataatgag cgttggggga tgaggcggtt tttatatttt
      241 aataatgctt cgggaaaccc tgcgacccaa gatccaaact ctgctattga acactataat
      301 tacctcacgg gattttggaa agacaactca ccgttgtgct acggaggtac aggtcatact
      361 tctggtggtg gcgatgcaag tgttcctacc gattttatgt tccccggaaa accgactact
      421 gatatttgcg gctggggtca gggtggtgta attatgcctg actggtcaga agaaactgaa
      481 ggaaaccctg caggtgaccg taggtttgtt caatctgccg gtccgtttat tctgaaaccg
      541 ggtgctgtga acgatattac aattggcgct gtttatgcaa gagctgcatc cggaggtcct
      601 tgggcttcgg tagaagctgt gagaaaagct gatgataagg cccagatact tttcgaaaac
      661 tgcttccgcg ttcttgatgg tcccgatgct cctgaaatcg aaattattga aatggataaa
      721 caactgattt tccacatttc aaataaaacg aattccaata actacctcga agaatatatc
      781 gaaaaagatc catcaattgt ttgtaccggt gaatatgaac cgtgcgacga atattatcgt
      841 tttcagggat atcagatatt tcagttgaaa aacgaatcgg taagtatcag cgacagatac
      901 aataataacc ttgttcgtca ggtcttccaa tgtgatttaa aagatgaaat tgaacaaatt
      961 gtaaactaca catggtcaga tgaattgggt gcaaatgtgc ctgttgaaga agtgaatgga
     1021 ttaaatgaag gtataactca cacatttgtg attaataccg ataactttgc taccggcgat
     1081 aaccgtttgg taaatcacag agaatattac tacacagcta ttgcttatgg atataataac
     1141 agtatgctct ataatcagca ggtgcaggaa acatttaacg gacaaaagaa accctatctg
     1201 gcaggtcgta acaatattaa acgctacaaa gccgttcctc acatcaacga cccggaagac
     1261 ggcggaacaa taattaatgg tgaatatggt tatggtcctg aaattaccca gatcgaagga
     1321 cacggtaatg cattcaatgt tttggatctg aaacaggaat caatcgacga aattatgtcg
     1381 ggacaccctt ggaaagccga aaaccctgtt tacgaaaacg gtaaaggtcc tgttacagtg
     1441 aaaataatcg atcctctgaa tgttcccgaa gattcctata ccttgcaact tgttgatgca
     1501 gcaatatgga gttacggatg gattaaggaa ggtgcaacat ggtacattgt aaatagtgca
     1561 ggcgatacgg tgaaatcaga cgttagcctg aatttcattg gaaatgaaca gcttattcct
     1621 gactgggggt tgtcggtgaa tgtggttcag accgaatatg caggcaacag aaacaaagtt
     1681 aaaaacaacg gctttctaga agcaagctat gaatttgaag atgagacaaa accatggctg
     1741 tacttcctgc ccgatggcga tggcaacgac gaatacaact ggataagggt tggcacctat
     1801 cagaataagg aaggcgaact tgaagcagga ggagccgata agagttacga cgattatatt
     1861 ggttacgatc ctgatgaaaa ctacgaaaaa atacttcagg gcacctgggc tccctttaaa
     1921 tttgcttccg atttcaaatt cggacctgcc tacaaagctg cgcgttcgat tcagaatatc
     1981 gaaaaatatg aacccatcag tagtgttgac attataataa ccagcgacac cagcaaatgg
     2041 actcgcgcct gtgtagtaga aatgtgtgaa aatgaatgga cagttgatga gtacaatcac
     2101 cctgaacagg taactccgtg ggtaaattat ttgtcggaag gcaatgcttt gagatttaca
     2161 cttcgtaaac agggctcagt cgataaaaac ggacaagctt tgaacgatgg aacccatggt
     2221 ttgggttggt tccccggtta tgctatcgac gtgagaacgg gagaacgtct caatatcgcc
     2281 tttggagaag acagctggct ggttggagaa aacggacgcg atatgatctg gaatccttcc
     2341 gaaggaattg caagtaatac aggtccactg ttcggaggta agcattatat ttatgttttt
     2401 ggtcataacc aaaactttac caaccctcag ttgcttgcac ctcggtacga ctcctgtgca
     2461 tttatctaca gtaaattgct taattatgaa acttctaaca atgcactgga tttacgcgca
     2521 gcgtggacat catgtatgtg gacagcaatt ccactgaaaa atccgagttt cgatttgttg
     2581 tcatccgatg ttaaaatccg actgagagtt gttactccgt tgcataaagc actgggcaat
     2641 ttcgcagttg aagagccggt taacgataat ttgccgatgt atactttcag cacctatgac
     2701 gttaaaacga ttaacaacga caccgttcag gctgttagtg cacttgacct tataaatatc
     2761 gttccgaacc catattacgg acacagctat tatgaaacaa accagctgga aaactatgtt
     2821 agaattacca atctgcctcg taaatgcact atatcaatat ataatgtagg cggaactttg
     2881 gttcggcgtt tcgaaaaaga ctctgatata acctatatcg actgggattt gaaaaatacc
     2941 tataacattt cgattgccag tggattgtat attgttcatt ttaatgtacc gggagtagga
     3001 gagaaggttg tacgatggtt tggtgcatta agaccgctcg acttaaataa tttctaacaa
     3061 gtaattgcaa ataattaata gataaaaata tgaaaaagtg ctttagccaa atagtaagcg
     3121 taatgattgc aggtttgttg cttttacctg ccctgagttt tggtggaaac gagcagcgtg
     3181 ccggatccag cggagcatcg gaactcctta taaatccctg ggcacgaagt tcgggatggg
     3241 gcgatgcaaa tgtagcctgt gttatgggtc tcgaaggtat gtaccagaat atagcaggaa
     3301 ttgcttttac ccgtaaaact gaacttatat ttaacaatac gcaatggatg gttggctccg
     3361 gtgtggtaat caataacttt ggttttgctc agaaagtggg agaaacagga gttctttctg
     3421 tgggcgtgat gaatatgaat ttcggcgaaa tagagattac aacaccagat ttgcctgaag
     3481 gcggtattgg tacatttaaa ccttcttaca ccaatattga gcttgcattt gcaaaggagt
     3541 tttcgcacag tatatacgga ggtataggcg ttaaaattat caacgaagga gttgccaatg
     3601 ctaccgcgtc aggatttgcc attgatgcag gtattcagta cgttactggc tttggaaaag
     3661 acaaagcagg aaacaggaag cgcgataact tacgctttgg tattgcaatg aaaaacgttg
     3721 gatcgactat gaaatttaaa ggtgatggta tggctttcac aggaattgtt cccaatggaa
     3781 caagcatgac cgttgaacac agatcgcagg aattcgaact tccttcgctt ataaaaatcg
     3841 gattgagcta ttccataaat cttgacgcta aggttgatag tgtgagtgaa acagtgattt
     3901 caaatcatca tctgactatt gctgcaaatt tcactgcaaa ttcatttacc aaagaccagt
     3961 ttcattttgg aatggaatac ggatttaaaa acatattctt cctgcgcggt ggatatatgt
     4021 acgaaaaagg tattactgat gtaaacgata gaactactgc cctaaccggg cctacggctg
     4081 gtgtgagtgt gcagatcccg atgaataagg agaaaaaatc cgttattgcc atcgattact
     4141 catatcgcac tactcaacct ttctcgggaa ttcatacttt tggagccagg gtaacattat
     4201 agcaataaca taacaattgt atagaaggga ctgaatactt gaaagtccct tttttattta
     4261 atcataattt attagtaatt aggaaattga agtaaaaact atgtttaata aaagaaatta
     4321 atgattgttc gaattaaaat attcgtatct ttgcagtaag aaacctcttg aaaatggggg
     4381 tttcttttgt tttatatatt ttttagaatt atgagcaaaa taacctacat gaccgaagaa
     4441 ggcctaaaaa agctgaaaga tgagctcgat cggcttacct ctgttgaaag gccatctatt
     4501 agtaagcaaa ttgctgaagc acgcgataaa ggcgacttgt cggaaaatgc ggaatacgat
     4561 gcggctaaag aagcacaagg aatgcttgaa atgaaaattg ccagacttca ggaaaccatt
     4621 attaatgccc gcgttatcga tccggctaaa atcgataata aaaaggtgca aataatgaac
     4681 agagtaaaaa ttaaaaacct gaaaacaaat gcaatattgg agtacatgct tgtttcggag
     4741 aatgaggctg acctgaaaga gaaaaagata tctgtgaatt caccaatagc aaaggggtta
     4801 attgggaaaa agcaaggcga cgtagtcgaa atacatgttc cggccggtgt gcttaatttc
     4861 gaaattatag aaatatcaat gtaatatcgt gttgcaatgg gttcaatctt ttcaaaaatt
     4921 attaatggcg aaataccatg tcacagaatt gctgaaaacg ataagttcat agcctttctc
     4981 gatatacagc ctttaaaatc agggcatacc ctgattgttc ctaaaaagga aaccgactac
     5041 attttcgatg tgaccgactc cgatttggca gatatgatcg tctttgctaa aaagattgcc
     5101 atagccatta agaaagcagt gccttgcaaa cgcattggcg ttgctgtcat cgggttggaa
     5161 gtgccccatg ctcatataca tctcgttcct ctggatgcag aatctgattt gcgattttcc
     5221 aatacacgcc tgaaactttc tcaggagcag ctatcagtta ttgcagaaaa aatcacatca
     5281 tttctggatt aatattctga ctgagaattt cgtttgccgt tcaacagtaa tatgttgaaa
     5341 tattcgtcag atcaacacaa tcaaaacaat ttataaattt taatttagta ggttatcatt
     5401 taatatgact gaaaacctta aaataataat ttatgctgat ggagcagcaa gtggtaaccc
     5461 cggcccggga ggatatggag ccattttaat gtcagggcgg cacaaaaaag aaataagcag
     5521 ggcattccga atgacaacaa ataatcgtat ggagcttttg gcagtgattg atgctttatc
     5581 tctgataaag aaagtgggtg cagatgtcac gatttttacc gattcacgtt atgttgtcga
     5641 tagcatcgaa aaaaaatggt tgaattcatg ggtggcaaca ggttttagga aaaagaaaaa
     5701 tgcagatttg tggcagcagt atttagatgt tgccgcacgc cataatattg tattacattg
     5761 ggtgaaaggt catgccggta atgagctaaa caaccgttgc gatgaactgg ccgttaatgc
     5821 gtatcgtagc ggtgtatttg aaattgacga agtatatgag cagcaaagaa acgaaatata
     5881 atacggaact acgtgccgtt aggttttcgt atgataacga cgaactgtta cgtattgccc
     5941 taaccatccg taaattggta tttgttgatg agcaaaaaat tgatccggat ttggagtttg
     6001 atggctatga taaagatgca caacactttt tattgtattt taaatcagaa cctgtagcta
     6061 ctgcccgatg gcgggaaacc gatgcaggaa tcaaactgga gcgatttgca gtaatgaaac
     6121 aattccggaa ccacgggtac ggtagcaaac ttatggcgtt tatcatgcag caaaacctta
     6181 caggaaaaca cttgtatttc cactctcagc tgtcggctgt tggattttac gaagcattgg
     6241 gtttcgaaaa ggatggggaa ctgttttacg aagccggtat tccccactat gcaatgaaga
     6301 aggcgacaaa accaaactta caatgacagc aaatccaatt aaacgacttg cagggcaaac
     6361 ggctgtttat ggtttaagca gtattattgg gcgactgctt aactatttgc tggtacccat
     6421 ttatacacgg ttatttattc ctgaggttta cggggtagta accgaactct atgcttatgt
     6481 aagcttttta ctcattttgt tgacctatgg taccgaaacc ggttatttca ggtttgcaca
     6541 ggacaaagaa cggtttgcca caacatactc tactctccta agtagcctgg ctgtaagctc
     6601 actggctttt atcggactga tttctatttt cattcatccc attgcatcgg caatgcagta
     6661 cacggcacat cccgagtaca ttatttggat ggcagtaatt gtagcaatcg atgcgtttat
     6721 ttcgataccg tttgcccgtt tgcgactcga aggcaaagcg atgaaatttg caagcctgaa
     6781 gctaatcaat attgccatca acatagccat gaatctgtat tttttgttgc tgtgtccata
     6841 tatcgcttca aaaaatccgg aaagtctatt ccttatcatt tggtcaccag gaataggtgt
     6901 agggtatgtg tttatttcca acctgattgc gaatataact acgttgttga tgtttattcc
     6961 ttcgttcatc aaggtgaaat atcgcttcga cgtacagttg ctgaaacaat tactactcta
     7021 ttcattccca ctcctgattg ctggttttgc aggaatgatt aacgaaactc tcgacaggat
     7081 attgctaaag tattgtttgc cggatcaggt aaatgcaatg gagcagattg gaatttacgg
     7141 agccaattac aaattggcaa tactaatgac actgtttgtt caaatgtttc gttatgcagc
     7201 ggatcctttc ttttttgctg aagcgaaaaa atcggatgca aaggcaacct atgccttagt
     7261 aatgaaatat ttcgtaattg gtggactgtt tctattcctg ggagtaatgc tatacctcga
     7321 ttttgtgaag tattttatcg accctgaata tcattccggg cttatggtag tccctgtgct
     7381 gctcatggca aatttatttc tgggtatgta ctacaacatg tcgatttggt ataagttaaa
     7441 cgataagact atttatggag catatatagc aattattggg gcctttatta ccattgcact
     7501 taatgtagct cttattcctt tttttggttt tataggctct gcctgggcaa catttatatg
     7561 ctacctaaca atgtttatta tttcgtattt gctcgggcag aaacattatc cggttaaata
     7621 ccctgttaaa gctattttgc tctatttcgt cgttgcgctt gtcatatact ttatcagcat
     7681 tgtaataccc ccaacaacag ttttcctgca ttatgcgttt aacacggtcc tttttgttgc
     7741 atttgggatt tttgttgtga ttagggaagg cttgtataag aaatatatat tgccaaagct
     7801 gcgttagata ccgtttgaat gttagaattt ccgtaatatt gtagattgaa caaattcatg
     7861 acaatgggaa aagttaaagt taaaatcatt aataagtcga aacatcagtt accgacctat
     7921 gcaacagcac tttctgcagg aatcgatctt agggcaaacc ttaatgagcg ggttgttttg
     7981 aaacccttgg aacgcaaact cattccgacg ggattgttta ttgaattacc ggaatcgtac
     8041 gaggctcaaa tccggcctag aagcgggctg gctataaaaa agggaatcac ggtgttgaat
     8101 actccgggta cgattgatgc tgactatcgt ggcgaagtaa tggtaatcct tgtgaactta
     8161 tcgtctgagg agtttgttgt tgaggatgga gaacgaattt gccagatggt agtagcccgg
     8221 catgcaacag ttgagtggga acaatccgat atactgaatc aaaccgaaag gggagatggt
     8281 ggttttggac acaccggaaa aaaataataa aatgatttac agtaatttaa aggaaatact
     8341 atgaaaataa taataccaat ggcaggtctg ggaactcgta tgagaccaca cacactcact
     8401 gttaaaaagc ccctgattcc tattgccggg aaaactattg ttcagcggct tgttgaagaa
     8461 attgcttctg taattaacca acctgttgaa gaaattgctt atgtgattgg taggttcggc
     8521 gaggaggtag aatcagagtt gcgcagcatc gcttcaaaaa ttggcgctaa agcaagtatt
     8581 tattatcagg atattgcgat ggggactggt catgcggttt ggtgcgcttc tccatcactc
     8641 aacggaccac tggttgttgc atttgccgac acattattct atgcgggctt taagttcgat
     8701 gcatcaactg atggaactat ctgggtgcaa aaggttaaaa cacctgaatc gtttggcgtg
     8761 gttacacttg ataataataa cattataaac ggttttgtag aaaaacccaa atcctttgtt
     8821 tcagatttgg ccattattgg gatttattat tttaatgacg gcttaaacct gcaaaaagag
     8881 ctccagcggc tgattgacga gaatataaag ggcaataatg agtaccaact taccgatgct
     8941 cttgaaaata tgcgcagaaa tggaatgaaa ttcatccccg gagaagttga tgaatggctc
     9001 gattgtggta ataaggatgc tactgttttg acaaatcaac gcattcttgc ccacgaaaag
     9061 gaatcactaa ctcaggttga aaataataac ataataaatt cagtgattat tcagccttgt
     9121 tatcttggac acgacgtaaa aatcgaaaac tctgtaattg gcccatatgt ttcagtaggc
     9181 agaaacacgg ttattagcaa ttgtattatc aaaaactcaa tcattcagaa caattctcag
     9241 ttaaatggtg cgtttttaca taatagtatg attggaaacg atgtcattat tgataaaagg
     9301 cctgatgatt taagcgtagg tgattttaac cagatacgga agaattcatg aacaaaaacc
     9361 tgacagcata tatagtttta gtaattactg ccttactttt gtattcgtgt aaaacaatgg
     9421 agaaatcacc cgttgctaag ggtaaagata ataccagtta ttttgccttt aagagcgagc
     9481 ttctcgatgg tgttcgaaat aaaatgacag gtaactatga tgctgccctt gataactttt
     9541 cgcgggcatc acggtgggat ccgaagcagg cagtaagcta ttacgaaatg gcaactgtat
     9601 tggcaatcaa gaacgattac ccagcagcac tggaatattg taaattggca accggtctgt
     9661 cgaaaaacaa ctattattac acactgctgc ttgcaagttt gtatcaggcc aatgccatgt
     9721 ctaaagaagc cgccgaaacg tatgatgagg ttatccgcct gaaaccttct atccccgata
     9781 actatttctc gcaatcatca atttatcttt cggtttctga ccctaaaaaa gctcttcaaa
     9841 gtcttgataa agcggaagaa tattttggtg taacacatga aataaattcc gagaagtacg
     9901 atattgctgt aacatcccgg aattatcctg tagcactgaa agaaataaac aaacttattg
     9961 aagcctaccc caaggaaagc atttattacg caatgctggc agaattatat aaagtcatgg
    10021 gaaaaactgc caatgttgcc gaggtatatc aaatgctgga aggtatggat gtccgcaatg
    10081 ggatggtcct tgtttcgctg gctgaatatt acagctcgca gggtaacaac gaaaaagcat
    10141 ttgctatttt ggagaaagca tttgcgcttg atgactttga tatggattct aaaattcaaa
    10201 tcttgttcaa cctgatagta cctaccgaaa atgatgtgat gcttaccgaa aaagtgtacc
    10261 ggcttcttga aattctggtt ctaaaatatc ccgatgaacc taaagtgcat accatatatg
    10321 gcgattacct ggttagggat agacggtggg aagaggcgca atctcggttc aattttgtgc
    10381 taaaaaacga aaagtcgcac tacgaaattt gggagcaggc attgctcatc gacaatcaga
    10441 taaaagactg gacttcgatg tatgacagga gtaacgaagc attgcagtat ttcccgatgc
    10501 aggttactct gtatgttttt aatactgaag cagcaatgca attgagtaaa tatagcgaag
    10561 cagtaatggc tgctgagtca ggattgcgat ttgcagcagg gaatgatgat atcaaactcg
    10621 atttctatat ttaccttgga gaagcatatc ataaaactaa tcagcatgaa ctgtcggatt
    10681 ctgcatttga aaatgcattg aagcttgact ctcaaaatac ttttgttctc aacaattaca
    10741 gctattatct gtcgctgcga aatgaaaggg ttgacaaagc tcttcaatta tcggaaaacc
    10801 tgatgctgct cgaatcggaa aatgccaatt tcatcgatac acatgcatgg gcattgttcc
    10861 gcaatcaaaa atataacgat gctctgattt acattgaaaa agctgtgatt atcgatggtg
    10921 aaaagaatcc gaatattctc gaacattacg gcgatattct ttctaaaacc ggaaaaaccg
    10981 aaaaggcagt tcaatgctgg aaaaaagcaa aagaaatggg ttcaggatcg aaaaccattg
    11041 atttgaaaat cgaaaaacag caatacaccg aatgagagga aagacacttg ttaaattagt
    11101 tttactgata gtcccacttt ttttaggtgt tggttgtagc aaggtgtttt atacaggtgg
    11161 gataaaaaac aactacaaac ccgttaagct ttttcagaaa atcagtgaaa gcgagattca
    11221 gtatcatacg tttatgctga aagctgactg cgagttcgaa gtgaacgggc aaagccagtc
    11281 gtttcagtta aatgctcgta ttcagaagga cagtgttgtt tggctatcga tgaaaatgct
    11341 tggagttgaa gggatgcgcg tgcaatgcct gaaggatagt ttgtatattg tcgataggat
    11401 aaacaatcaa tggtcggtcg cagcatacaa agaccttaac aatgtcttca gtatggagtt
    11461 gtgttatcaa ttcattcagg ctgttattac gaatacgttc tttttttacc cccctacatc
    11521 cgatactctg aaaacgcttc acgattttga taattgtaaa gatactttga attattgtat
    11581 gagttcaggt ggttggaagc ggtatcaacg atatatttca gacagcacag ggtccagacg
    11641 caacgaaaat cgaccgggaa gatacaaccc cgaaaggtat cacgataatg agcaagaagc
    11701 gggtgaagca tcgatttttc aggtgataaa ggtgtatccc gaaatattta aaattgctta
    11761 tgtgttgatt gacaattata tgaatggtca aactatggaa atttcttatg aagagcctcg
    11821 attgtttggt acaaactttt tccccggtaa aattcgcatt tggctcgatt ctcctgaaac
    11881 agaaataatt ttaaacatcg aaaccgaacg tattgaagtg gatatcgatc agcgatatcc
    11941 tttcaaaata ccttcgaatt acgaaagaat gaaataatag tattaattta gagagttgtt
    12001 atttaatatg ataaaacgaa ttccaatagc aaaagcgggc atttttttaa tgcttttctg
    12061 cctcatggct tccgtaactt tttcgcaatc gcgaaaggag ctcgagaaca aaaagaagaa
    12121 agtgcttcag gatatcgatt atacaaataa acttctcaaa gaaaatgaag caaaaaaggt
    12181 tgactcttac aacaatttat tgctgataaa caaaaaggtt gaatcacgac aggaattgat
    12241 tttaagcatt ggcgaagaat tggctataat tgatgaacat atcgacgaca acaaaatggt
    12301 tcttgatttg atgcaggaag atttgaaacg gctcaaggat gagtatgccg aaatgattta
    12361 ttttgcatat aaaaacagga atacttatag cagtattatg tttatccttt catcggaaga
    12421 ttttaatcag gcctatcgaa gactgatgta cctgcagcaa tattccgact accgtaaacg
    12481 acaggtgctg gctattcaat caacccaact catgattgaa aatcagatag caaaactcga
    12541 tgctctgaaa gcagagaagc aagccctgct gggcgagaaa aatgctgaaa aggagcaatt
    12601 aaaggaggaa caacaacagc agtcaaaaac gctgaataaa tacaaaaaca aagaagccga
    12661 gcttaagaaa cagcttaaga aacagcagga aagtgctgca cagctacagc gtgaaatcga
    12721 aaaaatgata gcagaagaag taaataagtc gtcgggtggg aagtcaaata cctatgtgct
    12781 tactccttcc gaaaagctgg tgtcaacgaa ctttgcaaat aataagggaa aacttccttg
    12841 gcctgttgaa aggggtgtaa tagtttcttc gtttggcaaa cacgcccatc cggttctcga
    12901 aaatgttacc atcgataatg cgggtatcga tatcagcact actgaaggag ctgttgtaag
    12961 ggcagttttc gaaggcgagg ttaggcgggt atttacgttg cctggtgctc agaatgctgt
    13021 aatcataagg catggaaatt atctgtcggt atatacacat atcgctaagg tgtatgtaaa
    13081 taccggagac aaagtaacaa ctaagcaagc aataggttcg gtttataccg ataaggaaga
    13141 gaataaaact atcgttcacc tcgaaatatg gtacgaaagc tcaaagcaaa acccggcttc
    13201 gtggctggca aaatagcgca aaatggaaaa gctggttagt gatattattc ttaagattga
    13261 aggcttcatc aagaagtact acacgaatag tattattaaa ggacttattc ttgctttttc
    13321 aatcagtgga ttgtattacc tgataattgt acttgcagag tatttcggac attttacggt
    13381 ggctattcgg acccttgcct tttataccgg tataatattg tttagcattg tttggatcta
    13441 ttatattatt attccactca ttaagctaat cggtatcggt aaacgaattt cgtacaagca
    13501 ggcttcggta atactgggta ctcatttccc tgaaatcggc gataagttgc agaatactct
    13561 tgaattggca gagcttatca agcacaaccc ggatgcgagt gacctgattt tggcaagcat
    13621 tgcacaacga aaaatgctga taagtccgat tcctttccgc aacgctgtac aatatcgtaa
    13681 aaatgttaca taccttaaat atttgttgcc ggttgcggca gtttttgtgg tacttctcat
    13741 tttatggcct tctattatca gtgagggaac tcaacgaata gtaaggcata gccaggaatt
    13801 cgttttaccc cctcctttta gcctcaatat cgatagcgat acgcttcttg ttaaaaaagg
    13861 caccgatttc aatgttaaag tgcaggtgga agggaatgtg gttcctgcag aagtttatat
    13921 acacattggc agcaacaggt acgtaatgaa gaaagacaac ccgagagagt tttcctatgt
    13981 tattcgcaac ctgaacaata acattcttat tcatgccagt tcaggtgata ttatttcagg
    14041 tactgttgag ctaaaagtat tgccatcacc agctatattg aatttttcac tgaatgttgt
    14101 tccacccgca tataccggaa ttgaacagaa actgtacacc aacacaggag acgtgtcagt
    14161 accctgcggg tcggagttga tttggcagtt tggtactgca aacattaata caatgaatat
    14221 gctggtcaac gatagcatcg aaattgccgg agagagaaat aacaatacat tttcgttccg
    14281 gaggaaaatt attcaacctc tgaagtataa tatgctggca tcgaatgagt attttaaaaa
    14341 tcaggaaaat gtggcatata ccattaatgt tatccctgat gtttacccgg ggataagcgt
    14401 taagcaggta caggacagcg ttgacctgaa tatatttttc tttagtggta caattgatga
    14461 tgattatggg tttaacagcc tcagttttca ctacaagacc gatagtcttc atgttgcatc
    14521 attgcctgtt ggcagagggg ttacgactca gaatttttac tatgctttcg atttttcggg
    14581 catcaacatt cccggcaact cagtaactta ttattttgaa gttggcgaca acgatgctgt
    14641 gcatggtagc aaaaagacac gctcaaccag ttttcagttt acggtaccca ctatcgaaga
    14701 aattgaatca aaaactgagg aagccaataa aagcattgcc tctaagatgg atcaggctaa
    14761 aaggcttgcc aatgagattc agcgcgacat aaataatctt caacggcgga tgatgaacga
    14821 aaacctttcg gaatgggaac gcaatcagat gatgaacgaa atactcgaaa aacaaaaaga
    14881 acttgaacag ctggcaaacg aaattgtgga agagcaaaaa aaatcgaacc agtataagaa
    14941 tacatttaac gataaagaaa acttgctaga aaagcaaaaa cagattgaag aattactaac
    15001 ccaattgatg gatgaagaaa tgatgaagct gattgaggaa ctgcaaaaac taatgcagga
    15061 attcgatcag gataaattta acgaattggc caacgattta aatcagaatt acgaagagct
    15121 ctcaaagcaa atggatcagg atttggaact ccttaaaagg atggaggtgg aggaaagaat
    15181 ggaaaatacc atcgataaaa tggatcagtt ggcagaagac caggaacaac ttgctgaaga
    15241 aacggagaat aaagagctga gcaatgatga actccttaaa aagcaacagg aacaaatgca
    15301 gcgtttcgaa gacctgcaga aggaatatga aaacaccctc gaaaaaaaca atcaattgaa
    15361 agagcctttt caaatggatg acttttcgaa ggatttcgaa gaaattagca atcaaatgca
    15421 gcaatcagaa cagaatttga acgaaaataa aaacaacaaa gcctcgaaaa gccagcagca
    15481 aagctcacaa cagatgcagt cgcttagtgc gaaaatgcaa aatatgatgg acagcaatat
    15541 gcaacagcag gctgcagaaa acctggatga tttgaggcaa atactcgata atttgatttc
    15601 gttcagcttc agccaggaaa aactgattaa cgaaattaat ggtgtcggaa ccagaagccc
    15661 tcggtatcgc gatattatag cggaacaaaa gaaaatcggc gatgatttca atatcattcg
    15721 cgattctctt gatgcgttgg caatgcgcat gcccgaagta aacgcgattg ttaaaaagga
    15781 agtaaataca atttttcgta aacttgatga tattatgtcc ggtttctctg aactcggtac
    15841 acaaaatatt accaccaacc aacagcttgt tatgacatcg gccaacaacc ttgcattact
    15901 attggaggag cttatgaaag ctatgcagca acaaatgtcg atgcaaatgt gcggcaatca
    15961 gaattgcagc aaacccaaca acagttcgca accccagatg ggtcagcttc gcgacttgca
    16021 gaaagggatg aaacaacaga tacagcaaat gattgatcag atgaaacaag gaggtgatag
    16081 cccggcacaa aaggagcaaa tgaataagaa tctggcaaaa atgcttgcac agcaggaaat
    16141 aatgcagcaa atgttgaacg aaatgatgac cagcgaaaca ctaagtccgg aatctgcaaa
    16201 aattcttaat gaaatcaacc gcatgatgga ggaaaatatt aaggacatga ttaatggtaa
    16261 tgtctcggaa caattgctac gccgtcagga gcttattctt accagaatgc tggaagtcga
    16321 gaaaagtgaa cacgaaagag agcttgatga gaaacgcaaa tctaacgaag ccagagatta
    16381 caaaatcagt aaccctgata aggcttttga tgagaaggaa aagcaaatga gatttaacga
    16441 gcttctgagg ctttctaatc ttaaaatgac acagtattac aaagaaaaat ataaaaacta
    16501 tttgcaaaac ctcgaaaaat aaccaatggc cgtaagcttt ttttcagaac aaattgctat
    16561 gcctgcattg aaaaagcggg ttattaaaac atgggtatca gaaataattg aaaaccacga
    16621 aaaaaaatcg ggaaacataa attttatttt taccaccgac gacatgttgc tcgaaatcaa
    16681 caaaaagtat ctaaatcatg attactatac cgatatcata actttcgact attctaccaa
    16741 caggctaatt catggtgata tttacatcag cctcgacagg gtaaatgaaa acgcttcaac
    16801 ccttaatacc ggtacaaccg aattgttcag ggtaatgatt catggggttt tacacctttt
    16861 gggctacgac gactccgata atgaaagcca agagaaaatg cgaaaggccg aagaccagtg
    16921 tattacgtta ctgttgaata agtaaagata ttgttcggtg aatagtaaaa atattacaga
    16981 aggcggttcc caaatcttat aactgaaaca aatactcttt ggcagcaaaa aactagcgca
    17041 tgtcaattat ttcggcattg ggaaaattgg ctgagttaaa gataatatcc ttatcggcga
    17101 ttaccggatt ttcaacaaat ttatcaatta ccacaaggta actaacccca tctttgccaa
    17161 cataatgtat ggaataaatc tgatttttag ttttatcaac tttaagtgtc agtttcgaat
    17221 atttcttccc ttcaatttta acaggatata aatctatttc gaccaaagtt ctgttgtttt
    17281 cgaacttatc gcttatccaa cggcatttat agtcctgcga gaaattattc agcacatttt
    17341 gcggattaaa tacggttcca tctgtttcag aggcatcggt gatatttatt tcttctgcat
    17401 cttttaggta agtccagttt gttttcccgt ccgaaaaaac cacttgtccc atcaggtcca
    17461 tcctatacct gtcgccacga tatagtgcct tgcctgaaaa ctgatctcta ctgccatcct
    17521 gaaggctttc cacataaaaa cgaaattcaa cctggatcga tttgtaagca cttagttttg
    17581 atgaaacggt ttgtagaagt tctatagctt tcgggtcgct ttgagaaaaa gccgaagagg
    17641 cagcaatgat taaaactact gttaagaata aatttttcat gttaggtaac tatttgtttt
    17701 cggagttctt caaatattgt tccaaagagt attcatcctg aaacataacc tggcgtgctt
    17761 tactcccctc gtaagggcca acaatgcccg cctgttcgag ctggtcgata atacgccccg
    17821 ccctgttgta tccaattttc attttacgtt gtatcagcga tgtggaccct tgctggtgac
    17881 gcacaatgag tcgcgctgct tcatcgaaaa gagggtcttt ctcatcatcg ttaaagctca
    17941 tatcgccgtt atcgcccgaa tccatccctg gatccggaag ttcataaata ttggaatacc
    18001 cttgttgttt cccaatgtag tcaacaatac tttcaacttc gggagtgtcg ataaaagcac
    18061 attgaatacg aattgccttg ccggtttcaa ccataagcat atcgcctttg ccaattagct
    18121 gatttgcacc tgttgcatcg ataatggttt ttgaatcgtg tcccgacatc actttaaaag
    18181 caattcgggt tgggaaattt gcctttatca caccggtaat aacatcaacc gaaggtcgtt
    18241 gtgtagcaac aatcaaatga atgcctattg ccctggcttt tgcagcaaga cgcgagatgg
    18301 gagcttcaac ctgtttacct tcctgaataa taatatctgc aaattcgtcg atgacaacaa
    18361 ctatataagg cagatattta tgtcctttca atgggttgag cttgcgcgaa ataaatttgt
    18421 cgttgtattc aacaatagtt ctcacaccgg ccatagtaag cagcttgtaa cggttttcca
    18481 tctcgatgca cagtgagttc attacaggga ctactttcga aacatcggta ataatggaag
    18541 cttcttcgtt gggaagtttt gcaaggaaat gtcgttcaat ttttgaatac aacgaaagtt
    18601 caaccatctt cggatcgatg agaacgaatt ttatctgtga cgggtgtttc ttatataaga
    18661 ggctggcgat tattgcattt ataccaaccg actttccctg accagtggca cctgcaacaa
    18721 gtaggtgtgg agtttttgca agattgaaaa cgaaagtttc gttgctgatt gttttaccga
    18781 tagctaccgg taactggaag gtgctttcct gaaattttgc agattttatt accgatttca
    18841 tcgaaacaat ctcagggttt gaattgggta cctcaatccc aatggttccc ttacccggca
    18901 ttggtgctat tattctaatc cccagagctg ccagactcaa ggcaatgtcg tcttcaagat
    18961 ttttgatttt cgaaatacga acaccgggag caggtactat ttcataaagc gttactgttg
    19021 ggccgatagt tgccgtgatt tttgcaattt caattttata atcctgtagc gttttaatga
    19081 ttttatcttt gttgctgttt agttcagcgg cagttacttc ggttttactg gcagcaatgt
    19141 cgataagcag gtcaatcgga ggttttttgt aattggcaag atcttccgta ggatcgtagt
    19201 cccccatcgg ctctatatcg atgtcttcat cagaaatcag ttcttcattt tcgttttctt
    19261 caagctcgag ttcaatgttg tcgtacaggg tttcagactc cggggtttct accggaaatt
    19321 caacaacgtt ttccgatgct tcttctgctg tcacctccga aacgtcttct tccatatcct
    19381 cttcttctga ttcgggaata tctccgaaca cagggttagc ttcttccaga tcgggctttt
    19441 ttaggtttct gattttactg ataccccgtt taatggcggg aagagcgctg tgaaaactgt
    19501 aaattattat cagaaatgca ctgataccaa tgcttgtcaa ggttcctatc ttcccccaga
    19561 aactgtttag ccatttacac ataaaatatc cgtgagcacc tcccagaaaa aatatcttgt
    19621 cgccaagaag aaacgataaa gttatactta accacaaagt aaaaatcaac agaataaaaa
    19681 ctgttttcct aaagggtagc agccgtatct ttagaagtcg aaaacccgca acaccaaaga
    19741 ttacaataaa cgaaaatgat gcaaggccaa accagtttct tatgaacaga tttgaaagca
    19801 gagcaccaat tttgccaacc cagttatcaa cttttaagct ttcattcgaa aatagcttgt
    19861 tcaatggaat atcgagaata ctttggtcgc ttttccatgt gaaaagataa gatgtaaacg
    19921 aaagcgtaag caatatactg aaagctatca gcagaattcc gaggcttact gtgaatcgtt
    19981 catcggtaaa aaaattcttc attgaaccga acattccggg cttttttgct ttaccctgct
    20041 ttttatcggt tttgtctttg gtattgcttt ctttcttcat gtaagtataa aaataggaaa
    20101 atcctcaaaa tatgagaatt ttccgcaaat atttttatga aaaatgaaaa aactatccac
    20161 caaacattgt tttaaactct tcgtatgatt ttttctcgta atcggaaggt acagtgaaca
    20221 aagattgatt cagttttgac ttgcttactt ctttaacaga gaacttcatt atcatgccat
    20281 tttggtccat gttgtattcc atgaaaatac catcgattcc ttccatctga tactgcgaat
    20341 ttttatcgaa gggaacagca agctcgttgg tgtaatacac ttccatcgtt tgcccaccgg
    20401 aggtgatttc agcttttttg caggtgtatc cggcaataac ctttgtttcg tcgagttgtt
    20461 tgataacagg tttttcttcg ctctcgtctt ttaaggcttc aagatcttct ttggtttgta
    20521 ctacgtaaaa tttcataccc attccgttaa acatttgggt aaccgaacca tcgttaacat
    20581 tcgacagtga tatgaatgaa aacataggtg taatcatttc agtacggata ttatcacctt
    20641 tataatatac gacaatttcg gatggcatct gtgctcttgt tgctgcatca atgttttcac
    20701 cttctacatc gattttatat ttcaccgatc cttcaaatgt tttggctttt ttctgtcctg
    20761 caacattcag ggctaatact acaaacaata acaaactgaa aattctgaca aggtttttca
    20821 tattaatttt aatttaagat ttaatgagtt tattttttca agatttgtac caaaaagtat
    20881 tttacaaaaa taattttttc tttattacaa cgaaatttat agggggcgta tttaataatt
    20941 aattgaataa cataaattta gttgtttgaa ttctcaaaat atttttagtt ttgtggttta
    21001 ccaaaatact gattcatgaa taaaaagtta gcagttgtgg cttttggggg aaatgcactt
    21061 cttcgcgcag gccaaaaagg aaccattgaa gaacaggaaa ggaatgtgta cgaaacttgt
    21121 gaaagcttgc tgaatctgat aaaaaatgat tataatcttg taataggcca cggaaacggc
    21181 cctcaggttg gtaatgccat gctttgcaat atcgccggta ataaaatgtt cgatttacca
    21241 ctaatgcctc ttgacatttg tggttcatat acacagggct tcataggcta tattattgaa
    21301 cagcagctta aaaatatatt attgaggaat aatatcgaaa aggatatatt aacgcttgtt
    21361 acgcaggttg ttgtcgataa aaacgatacg gctttccaga acccgacaaa accggttggg
    21421 ccatattata caaaggaaga gatggaagcc cttgttaaac agaatcccac cgaaacttat
    21481 gctgccgacc ccaaaggcag ggggtatcgt aaagtagtcg cttctccggt ccctatcgaa
    21541 ataaacaacg tgcattctat agaaatgctt gcccgaagcg gtaatattgt tattacagtt
    21601 ggtggtggcg gaattccagt gtactataaa aatgattcaa ccatcgaagg cattgatgca
    21661 gtaattgata aagacctggc ttcagctttg ctggcaacaa aaattaatgc tgacgaattt
    21721 tatatcctaa ccgatgtgcc aaaagtatgt ctgaattttc atactcccga cgaaaaacag
    21781 ctcgatgttg tttcgataaa tgaagcaaag cagtaccttg aaatgggcca tttcacagag
    21841 ggtagtatgg caccgaaaat cagggctgct attcgttttg tcgaaaacgg aggtaatgaa
    21901 tgtatcatca ccgatgcaac tcagcttggc gtgaaaaacg ctggcacccg cattgttaga
    21961 aatcactaaa tgttaaatat aaataaatct tagaactatg gcatttaatt taagaaacag
    22021 gaatttttta aaagagcttg atttcacaaa agaagaattg gtttttatgt taaatcttgc
    22081 aaaagaatta aagtctgcaa aatatgcagg tactgaacag caaaagctaa aaggcaaaaa
    22141 cattgctctg atttttgaga aggattcaac ccgtactcgt tgtgcatttg aagttgcagc
    22201 acttgatcag ggagctcatg taacatatct tggcccgaca ggaagtcaga tgggacataa
    22261 agaaactgta aaagatactg cacgtgttct tggcagaatg tacgacggaa ttgaatatcg
    22321 tggtttcgga caagctattg tagaagaatt ggcaaaatat gccggcgttc ccgtttggaa
    22381 tgggctgaca aatgaatggc atcctactca gatgatggct gatgttttga caatgattga
    22441 acattctgct aaaccattgg aacagatatc ttatgcttat ctgggcgatg cacgttataa
    22501 tatgggtcgt tcattactgg ttattggaac tatcctgggt atggatgtac gaatcggtgc
    22561 ccctaaaaaa ctatggcccg atgcagaatt gataaagacc tgtcagaaaa ttgctaaaga
    22621 atccggtgca agagttatag ttaccgaaaa acctgaagaa gctgttaaag gtgttgattt
    22681 cattcacaca gatgtttggg tatcaatggg cgaaccaaaa gaagtttggg aagaaagaat
    22741 taaactctta aaaccatatc aggtaaatcc tgcaataatg aaaaagtcag gtaaccctaa
    22801 tgtgaaattt atgcattgtc tgccggcatt tcataatgct gatactaaag ttggaaaaca
    22861 agtttcggaa gattttggtc ttaaaaacgg tgttgaagtt accgaagaag tattcgaatg
    22921 taaagcttcc cttgtttttg atcaggctga aaatcgtttg catactatta aagcagttat
    22981 ggttgcaaca ttgggtgcgt aaatgctttt ggaatattta aaataattct aaattacata
    23041 agtttttcag aactcattct tttttatttc gaaatgaatt atgtttgcga agttataagc
    23101 gacaatttat tattaaccta aaacgataaa aaaatggctt acaaaatttc tgacgattgt
    23161 actgcatgtg gaacctgcat tgatgaatgt ccggttgaag caatttctga aggtgatatc
    23221 tataagattg atcccgatgt ttgcacagag tgtggtgcat gtgctgatgt ttgccctgtt
    23281 gaagcaattc acccggaaaa ctgatcagtc aggtaaaaga attgaagagt gccagcgtgg
    23341 cactcttttt tttatatgct ggtgatattt gtatgctgtg cgtacaaaag cggaattttc
    23401 gaaacttatc aaataacata ttttaggtat tagttttttt ttaaattagc acactctaaa
    23461 aagaaatata ctcacaaaaa aattaaaaat aattaatcgt agaaacgatg gcaaaaacaa
    23521 aaaaatttat cacttgtgat gggaatcatg ctgctgctca tgtagcttat atgttcagtg
    23581 aagttgcatg catctatcca atcactcctt cttcgaacat ggcagaaaat gtggatgaat
    23641 ggtctgctgc aggaagaaaa aatattttcg gacaaaccgt aaaagttgaa gaaatgcaaa
    23701 gtgaagccgg tgctgccggt gctgttcatg gtgcacttca ggcaggtgcc ctcacatcaa
    23761 cattcaccgc ctcacaaggt ttgctgctga tgataccgaa tatgtataaa atggccggcg
    23821 aattgctgcc agcagttttc catgtaagtg ccagaacagt tgctgctcac gcattatcga
    23881 tttttggcga ccacagcgac gtaatgtcaa cacgaggtac tggttttgca atgttggcaa
    23941 ccggatcggt gcaggaaata atggatattg cgcctgttgc ccatttggca gctattaaaa
    24001 caagaatacc attcctccat ttcttcgatg gattcagaac atctcacgaa attcaaaaag
    24061 ttgaatatct tgagaatgac gacatggaag gcctcatcga tcaaccggct ttgcaacttt
    24121 tcagagataa tacactcaat cctgaacacc ctgttacgaa aggaacagcc caaaatccag
    24181 atattttctt tcaggcaaaa gaagcttgca acagctttta ttctatagtt cccgatgttg
    24241 ttgagtacta tatgaaagaa atgactaagc tcactgggag aaagtaccat ccttttacat
    24301 attatggcgc tcccgatgct gaaaatatag taattgcaat gggctcggta accgacacaa
    24361 taaaagaagt tattgatttc aaactaaaac atggagaaaa attcggttta atcacagtgc
    24421 acctttacag acctttctcg gaaaaatatt ttagaaaagt gctgcctaaa accgttaagc
    24481 gtatcacagt tttggaccgc accaaagagc cgggagcaaa tggagaacct ttgtatctcg
    24541 atataaaaga tattttctat agcgataaaa atgcgcccat gattattggc ggccgttatg
    24601 gactgtcttc aaaagacaca actccagcaa tgataatgtc ggtacttgat aatatggcat
    24661 caaaacaacc aaagagtgga tttactgttg gtatagttga cgatgttaca aaactctcat
    24721 tgcctttaaa gagtgacgtt accgtacttt ccaaaggtac attcgaagga aagttttatg
    24781 gactgggctc cgatggtact gtaggagcaa ataaaaacag tataaaaatc attggtgaaa
    24841 caactaataa atattgtcag gcctattttg cctatgactc aaaaaaatcg ggtggattta
    24901 ccgcatcgca tctgaggttt ggcgacaaac ccatccgtgc accatacctg ataaactcac
    24961 ccgattttgt ggcatgccat gtgccggcat atatttatct ttacgatgtt ctgaaaggta
    25021 ttaaacccgg cggaacgttt ttattcaact caatttggag tgtgaaggaa actttggaga
    25081 aactgcccga tcacatgaag aaaacactgg cggaaaaaaa gataaaattc ttcatgatta
    25141 atgcaacaga aattgctgaa agaattggtc tgggtaaccg tactaataca atcatgcaat
    25201 cggctttctt caggatttcc ggcgtcatcc cttatgaatt ggcagtaaaa gaaatgaaga
    25261 aaatggttgt aaaatcattt ggcaaaaaag gtgaatcggt ggtgaaaatg aaccacgacg
    25321 cgattgatgc cggttgttgc aactatgttg aggttgatat cccaaaagaa tggaaaaaac
    25381 tccccaacac aggctttaag gtaatacgca ataatgttgt ccctcagttt atatcgaacg
    25441 ttgccgatgt aattaactct cagaatggcg atagtttgcc tgtttcaaca tttgttggca
    25501 gagaagatgg cacattccca tcgggtacta ccaaatacga aaaacgcggt gttgcggtga
    25561 atatacctga atggattcct gaaaattgta ttcagtgcaa ccaatgctct tttgtttgtc
    25621 ctcatgctgc tatcaggcca tttttattaa ctgaagacga gttgaagaat gctcctgcag
    25681 gtactaaggc tattcaggga attggcggaa caaaggaata caaattccgg attcaggtaa
    25741 gtgtactcga ctgcacgggt tgtggcaact gtgctgacgt ttgcccggcc aaaacaaagg
    25801 cacttaccat gaagcccatc gaaacccagc gtgctgaaga agccagatgg acttatatgg
    25861 atgaagttgt tggttataaa gaccatattc ttcccaaaga taaaacagtg aagaacagtc
    25921 agttcgcaca accattattt gaattttcgg gtgcctgcgg cgggtgcggt gagaccccct
    25981 atattaaagc cataacccag ctttttggtg aacgaatgat tgtggcgaat gctacgggtt
    26041 gttcatcgat ttacggtggt tcagctccgt caacaccgta ttgcccgcat tataaatcgg
    26101 gctttgggcc ggcatgggct aattctttat ttgaagataa tgcagagtat ggttttggta
    26161 ttgccactgg aatagataaa atccgtgaga aaatttgctt gcagatagaa aatgcccaat
    26221 ggcccgaaaa ggctatgaat attgctcttg agtggaaaaa tggtaaaaac aatgcagagc
    26281 aatcgattga tgcatcggct aagtttgtcg aaatactgga atcagtcaac gataacacag
    26341 ctaaggaaat actgaaatac aagaactatc tgataaaaaa atcggtttgg atatttggtg
    26401 gcgatggctg ggcctacgac attggatatg gaggcttgga tcacgtgatt gcttcaggga
    26461 aagatgtgaa catacttgta cttgatacag aggtatactc aaatacaggt ggtcaggctt
    26521 caaaatcaac accggtaggt gctgttgcaa aatttgctgc tgctggaatg agaacccgca
    26581 aaaaagacct gggtgcaatg gctatgacat acggatatgt ttatgtggct caggttgcta
    26641 taggttcgag tcagaatcag ttcttcagag caattaaaga agcagaagcg tatccaggtc
    26701 cgtctttgat tattgcgtat gcaccctgta ttaaccatgg gctttcattg ggaatgggta
    26761 agacccagga agaacagaaa agggctgttg aagcagggta ctggcattta taccgctata
    26821 acccactgct tgaagccgaa ggacaaaatc cgtttgctct cgacagtaag gagcccgact
    26881 ggactaaatt caaagatttc ctgaagagtg aggttcgcta ttcttcattg ctgaaatcgc
    26941 ttcccgaaga aggcaaacat ctgttcgata ttgctgaaca gaatgctcaa tggcgataca
    27001 attactataa acggttggct ggaatttaat tttttagtta atgattaaaa aaatagagaa
    27061 ccgctgcagg ttctctattt tttttaagag aaattgtcat ttctaatgcg gtttttatta
    27121 gcttgcaatt ttattttg
//
LOCUS       DAOOQP010000027        26553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2180_length_26553_cov_5.828251, whole genome shotgun sequence.
ACCESSION   DAOOQP010000027 DAOOQP010000000
VERSION     DAOOQP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26553
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2180_length_26553_cov_5.828251"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLK75_04635"
     CDS             <1..296
                     /locus_tag="PLK75_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY38566.1"
                     /translation="FISQESMDAQQAALVKKQESLQALDEQLTQELLDRQKDMNKQLY
                     DTVMNYIKEFNEGQYCIILGNASGSNVLYAQEGMDITREIIDNLNDRYSKSKK"
     gene            309..1565
                     /locus_tag="PLK75_04640"
     CDS             309..1565
                     /locus_tag="PLK75_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOY38567.1"
                     /translation="MGFANHYFERFGVSGTLLSAPVAITPDIIVVIPVCDELGLPDCL
                     DSLASCTGHSSKILVIFVVNHGIHASDAVKNQNAETYRYIEECKNSMCREDFAIHELM
                     LGNVAQKTAGVGFARKTGMDEAIRVFNMLNKSDGIIASLDADCKIETNYFSALEEFFK
                     KNSNSGAAVIRFAHPLSGNEYPPEIYDSVCLYELYLRYFKHALSYCGYPFPIYTIGSC
                     FAVDAKAYIRSGGMNKRQGGEDFYFLHKLTAFANVGYISGTCVQPSPRISMRVPFGTG
                     PAIQKILNTGEWKVYNFALFHQLKQFFAIFKLFYYMDENAVYEKVDSINQQLGNFCRS
                     VDLAAKIQSIKLNTASSVSFEKKMFQLWNVFTIIKYLNSQSQGQFPLQKTENAAYELL
                     TALNCSVKTNHPKNLLEIFRAIDESV"
     gene            1624..2163
                     /locus_tag="PLK75_04645"
     CDS             1624..2163
                     /locus_tag="PLK75_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY38568.1"
                     /translation="MKKLIVVVLFILQITAVWSQKGAISGRIFDESNNTALPFSNIYI
                     SGSQIGATSDFNGNFLITALEPGIYQLTAQSLGYATVFTEEIRVMAGKTANINIGMRA
                     EALQLKEVEVKANVFRKVEESPVSLRTIGVSEIENNPGANRDISKVIQTLPGVAAFPG
                     QNRNDIVVRGGLRTKAGFI"
     gene            2349..4013
                     /locus_tag="PLK75_04650"
     CDS             2349..4013
                     /locus_tag="PLK75_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38569.1"
                     /translation="MRFRGSLGASEVALSLDGPLGENTTYLISARRSYLNFLFKLIGL
                     PFLPTFNDMQFKVKTRIDDKNEISFIGLASYDVNVLDKNIDDPTEYQRYLLGNISESN
                     QWTYALGAVYKHYRENGFQTIVLSRNMLYNRYYKYLNNIVDDAGLISDYNSFEAENKF
                     RFDNFSQTNNGFRFNYGVNAEYVKYNNNTFRKYYRFDMLDTLLYASNLGFFKYGISGQ
                     VSKSLFGNRLTLSLGVRADANTYSKEMSNPLPQLSPRFSVNFLLTDKLSLNANVGRYY
                     QLPPYTSMGYRDASGELINKNNNLKYIRADHIIGGIEYRPTQTSIVSVEGFHKVYANY
                     PMSLTDSMALAFMPVDFGIVGDGPVSSVAEGRAYGFEVLTQNRFGNNITLVASYTFSV
                     SEFKDKSGSMIPSSWDNRHVFSLIITKQFKKNWSAGLKWRFAGGLPYTPYDLETSAIR
                     SAWDIAGRPYFDYARLNGERFGAFHQLDIRIDKGWNFKRSSLKLYIDIQNLYNFKSET
                     QDIFVNTDINGNPVINPLDNSKYVLRALDKEGSGTVVPTIGIIIDF"
     gene            4025..4780
                     /locus_tag="PLK75_04655"
     CDS             4025..4780
                     /locus_tag="PLK75_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38570.1"
                     /translation="MKHIIIAAVVAIFATFAQAQDTPKKLCKKKQHEPENIVLNSQGA
                     GNNFIFTFTPGKYHNYPTLAIWIEDTEGNYIQDLFVTQSFAKGIFRYGAQQNGKWTEG
                     PRRYQATLPYYLHKRATALHLTDIVPDSKQPVPDAYTGATPGADFSLKVNSNEIMKEK
                     YRILIEINQAWDVNEYWYNNRFENDNDYKASCQPSLVYAVTIDPSDLMDEYVFNPIGH
                     GHYSGKDGRLYTDLSTLTTALKIFTKISVVVAP"
     gene            4885..5763
                     /locus_tag="PLK75_04660"
     CDS             4885..5763
                     /locus_tag="PLK75_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY38571.1"
                     /translation="MKNIHLLLLISALITSSCVYSAELKKKPAGDTVTCREIEKSVVL
                     ISGNLYAGKFEVSNKLYRIFLTNLMKIGADEKYSIAQIDSLNWRETRCGFAELFLEPY
                     VELYFRHPAYSEHPVVNVSFQGAQLFCEWLTGYYNSMPGRKYKKVRFRLPTDTEWEHA
                     AKGGLEYVLYPWGNRLIYNNQYACNYYHVGDENIIRNISTGTLIVNERKADASPGKLN
                     DFNSLTVPVTYYQPNEFGLYNVCGNVAEMVIDKPVARGGGWLSPGGDVTIFSSAVCTQ
                     SAIDTGFRYFMEVVEY"
     gene            5919..7334
                     /locus_tag="PLK75_04665"
     CDS             5919..7334
                     /locus_tag="PLK75_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY38572.1"
                     /translation="MKRTFIFLNLLIVLTLGVNAQKINFYVTGGYTFTRAPLKDVHEF
                     IGLYNENPNKIHGYVFTHGFGSLKCLNGIYLGGGMNFKGIFLAELNYTQKYRHTFAYY
                     DPANNPDYLRVELGYSISTVEAAFLVPYSFGKLTVAPGIGMGFLSRKMYYWDDDSLMI
                     APKRRDMSVESRRGSFSLDPMLQVSYKPFRKIPVEFVARTYYQAMFSKMSVSWLGDFE
                     GYWSPADTASKKVTGGNLGIILAIKVNIPEISFKHRSKNVEPVVIEPVYEEKTNFVLS
                     GTVQDAKTSLPLNAVITLYQGTNPIESVISQSGKFTLKPALNSDYTIETKAFGYQTKT
                     EKLRFDSKSPNPYKYNVKLSKIPAGQAIVLENILFEKSSAVLLPESYTELNKLLQFMN
                     DDSEIRIEIAGHTSSDGDDAYNMKLSQDRANSVATYLINKGIDKTRIVAKGYGETMPV
                     ASNDTEEGRRLNRRVEFKIVK"
     gene            7336..7917
                     /locus_tag="PLK75_04670"
     CDS             7336..7917
                     /locus_tag="PLK75_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38573.1"
                     /translation="MKYLFHKFSVFLTGILIFVIAQCQAQTIQVTYAVIGSDTLHSWF
                     SDTCIVMEKFSMPSMRYVDKIDFKKKEVIVQINQYNDFTRNTHNSIFTFKELKKKKPF
                     MPMLVKLTDETKVILGFECHKALVEKEQTHYDIWYTKLPQLPDLNYNFYSIYYQIPGV
                     IIEEWMNGNLIMHAVSVVEGKWCNYEWVDYQGF"
     gene            8060..9217
                     /locus_tag="PLK75_04675"
     CDS             8060..9217
                     /locus_tag="PLK75_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOY38574.1"
                     /translation="MNNFTFQNTTRIHFGKGQISKLDRELSPYTRIMLTYGGGSIMGN
                     GIYEQVKSLLKGKNVIEFGGIEPNPRYETLMRAVEMAKMQGVEFLLAVGGGSVIDGTK
                     FIAAAVPFTDGDPWRMLTEGAKIYHALPLGTVLTLPATGSEMNGNAVITRDSVKQKLA
                     FGNRLLQPVFSVLDPETMFSLPDRQISNGIADAFVHVMEQYLTYPVNSPVQDRFSEAL
                     LITLMEEGPKVLKNKTDYDACANLMWSATMALNGIIVAGVPDDWSTHMIGHELTALHG
                     IDHAQSLCVVLPGLMQHQRVQKAQKIIQYANRVLKINYDNNENTIDEAIRKTEAFFLK
                     IGIPTNLGHYDITAKNVDYLCDRLSENRQNAWGEHKDIDAEAVRKILFSRL"
     gene            9260..10390
                     /locus_tag="PLK75_04680"
     CDS             9260..10390
                     /locus_tag="PLK75_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38575.1"
                     /translation="MKKHLLLLPMLLICFSVFAQLNTRTLTWDGTARQYLEYVPSVYS
                     GETPVPVVFCLHGLGDNMTNFSQIGFDNIADTANFIVITPQALVAYYMSYEIGTSWNS
                     GASLYGIVPNADVDDSGFLMAILDSLQNHYNIDPERVFVMGFSMGGYMTNRLGSEHGD
                     RIKAIASVSGTFGIAFTPDPVGPISSLHFHGTADETVPYLNNTSGMDAEELVAYWVDI
                     NNASQTPEVYNYPNTKPDGLTFERYTYTGGDNDTYDCFIKVVGGEHNWYYYPNNDIDY
                     SIEIWRFFTNSMITTGIASVEHCESTLYPNPATDFINFDIAPDSNGIKSLQICDVTGR
                     VLCQCKANVGSNHLDVSSLPSGVYLLIVNDNESLKQFRFVKK"
     gene            complement(10400..10975)
                     /gene="gmk"
                     /locus_tag="PLK75_04685"
     CDS             complement(10400..10975)
                     /gene="gmk"
                     /locus_tag="PLK75_04685"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOY38576.1"
                     /translation="MQSNKAIIVSAPSGSGKTTIVHNLLNNKMLNLEFSVSACSRPKR
                     DQETEGKDYYFLSVEQFRSKIENSEFLEWQEVYNDHYYGTLRSEVDRVFAKGNNLIFD
                     VDVVGGLNLKEYFQEDALAIFIMPPSIEELERRLRLRSTEEDEDIAKRVKKSNFEIRY
                     AERFDLIVINDVLNDAIAEVTNAVIAFLQRK"
     gene            11169..12773
                     /locus_tag="PLK75_04690"
     CDS             11169..12773
                     /locus_tag="PLK75_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY38577.1"
                     /translation="MQKQVLLGDEAIAQGAIDGGVSGFYAYPGTPSTEIMEFVQASKE
                     AASKGIHCIWSANEKTAVESAIGMSYAGKRSMAQMKHVGVNVAADAFINASITGVNGG
                     MILVAADDPSMHSSQNEQDSRFYADFAMIPILEPSNQQEAYDMAYSGFELSEAFNVPV
                     MMRITTRLAHSRSDVERRAVKPMLNGSLPKDPRQFVLLPAIARKNYKKLLGMQQAMQE
                     ASDNSDYNRYFEGTDTSMGIVACGLAYNYLMENFPEGCKFPVVKISQYPIPKNHLTRL
                     YKTCKSILVLEDGYPLVEKMLRGFFESSVPIKGRLEGSLPRDGELNPNIVAAALNIAI
                     QEGQAVPDVVKNRPPSLCPGCGHRDMYNALNEAIAPYGTGRVFSDIGCYTLGALPPFN
                     AINSCVDMGASITMAIGAADAGLVPAVAVIGDSTFTHSGMTGLLDAIIKDSPILVIIS
                     DNSTTGMTGGQNSHGVGRIYNICKGLGVSEEHLHVVTPLRKYHEENTELFRKELAHKG
                     LSVIIPQRECIQTAARHKRETSKNSN"
     gene            12787..13374
                     /locus_tag="PLK75_04695"
     CDS             12787..13374
                     /locus_tag="PLK75_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOY38578.1"
                     /translation="MKTDIILAGVGGQGILTIAAIIGVAAVNKKMYLKQSEVHGMSQR
                     GGDVQSNLRISEKPIYSDLIPKGQADLILSVEPMEALRYLPYLSKSGYLITNLVTFNN
                     ITDYPEEARIFAEIEKLPRKVAIQADLIAKEIGSARASNIVMLGAASSFLNLSIEDLE
                     YGITSLFSKKGEEVVAMNIKALHAGRDAASQYLKK"
     gene            complement(13563..13934)
                     /locus_tag="PLK75_04700"
     CDS             complement(13563..13934)
                     /locus_tag="PLK75_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38579.1"
                     /translation="MKKITVLLGLVAFVFALVSCGGPKADAKKMFGMMKKYTETATKV
                     VEDKKIDDAEAADLNKLMKEMSDVSKKFEDKYKDDKEAEKIFEEMAKEPENEKMLTSL
                     MEVSFKLYECEGFDKLEEMDF"
     gene            complement(14057..14386)
                     /locus_tag="PLK75_04705"
     CDS             complement(14057..14386)
                     /locus_tag="PLK75_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38580.1"
                     /translation="MKNTVLTIATIILGVTFFVSCSDNSTAVVNKIKEFNSVATNAVQ
                     DSVLDDTEVQKLDALLAELNTYGEDTEAFEKTINKDENKAIYDEMNTLVIRLFSCDGS
                     DKLKNLQ"
     gene            complement(14426..14779)
                     /gene="rplT"
                     /locus_tag="PLK75_04710"
     CDS             complement(14426..14779)
                     /gene="rplT"
                     /locus_tag="PLK75_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOY38581.1"
                     /translation="MPRSVNKVAARERRKKLMKLTKGYFGRRKNVWTVAKNTLEKGLQ
                     YAYRDRRAKKREFRALWIQRINAGARMHDLSYSQLMGLLHHKNIIINRKVLADLAMNQ
                     PKAFDAIVKEAKSKK"
     gene            complement(14864..15061)
                     /gene="rpmI"
                     /locus_tag="PLK75_04715"
     CDS             complement(14864..15061)
                     /gene="rpmI"
                     /locus_tag="PLK75_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOY38582.1"
                     /translation="MPKMKTNSGAKKRFQLTGTGKIKRKHAYKSHILTKKSKKRKRNL
                     GYFTLVDSTVEKNVKQMLCMK"
     gene            complement(15088..15651)
                     /gene="infC"
                     /locus_tag="PLK75_04720"
     CDS             complement(15088..15651)
                     /gene="infC"
                     /locus_tag="PLK75_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOY38583.1"
                     /translation="MAENRNTGRVFKKADDNQFRVNHYITAPFVRLVGENVETQILSL
                     REALQVAQSLDLDLVEISPKAEPPVCRVIDYQKFLYQQKKKLKENKAKTQKVVIKEIR
                     FGPNTDEHDYQFKLKHAQRFLEEGSKVKAFVFFKGRSILFQEQGEILLLRLANDVEEF
                     GKVESLPKLEGKKMFLIINPKPGKKKK"
     gene            complement(15686..17623)
                     /gene="thrS"
                     /locus_tag="PLK75_04725"
     CDS             complement(15686..17623)
                     /gene="thrS"
                     /locus_tag="PLK75_04725"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOY38584.1"
                     /translation="MIHITLPDKSVRQFEAGVTGMEIAANISSRLAQEALAITVNNEV
                     WDLLRPIHTDASVKIHLWESEEGQSAFWHSSSHLMAHALEKLYPGIKFGIGPSVENGF
                     YYDVDLPEGAQLTDADFARIEQTMLDIARHKIEYKRRDVSKDEAIRFFTAKNDPYKLE
                     LINELQDGSITLYESGDFVDLCKGPHIPNTEKIKAVKILSIAGAYWRGDEKRKQLVRV
                     YGTSFPKQKLLDDYLVLLEEARKRDHRRIGKELELFTFSPKVGMGLPLWLPRGTELRK
                     RLEEWLTKELIRRGYQLVMTPHIGNVELYKTSGHYEKYGKDSFQVIQTPHEGEAFMLK
                     PMNCPHHVEIFASTQFSYKDLPVRLAEFGTVYRYEQHGELHGLTRVRSFTQDDAHIFC
                     RQDQVKDEFKSIIDLVLFLFRSLGFNDFVSQISLRDPNDKTKYIGDDENWNKAEQAIQ
                     EAAYEMGLDPVIAPGEAAFYGPKLDFIVKDAIGRKWQLGTIQVDYNLPERFDLYYKGA
                     DNEKHRPIMLHRAIFGSMERFVAVLIENTAGKFPLWLTPQQAVILPISEKFTDYAKKV
                     LELLKNYDIRSLIDERNEKIGRKIRDNELKRIPYLLVVGEKEESTGTISVRKQGVGDA
                     GSMSPESFAEMIRNEIIEFIK"
     gene            complement(17943..18842)
                     /locus_tag="PLK75_04730"
     CDS             complement(17943..18842)
                     /locus_tag="PLK75_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOY38585.1"
                     /translation="MKTNLSMAFVLFFIFNSTFAQTTKIKPSKGKVNKELRYKSVVLG
                     YDVHYSIYLPPDYDNSEKKYPVLYLLHGYKGNHKSWINLGRVNKIMDTGIATGEIEPM
                     IIIIPDGKYYWYINDYQSKARFEDFMFIEFLPFIDSTYKTIPNKESRAVAGLSMGGYG
                     SLVWAMKHPDKFSYCVALSASIFSEDGLVSMPEQLYKYMSMLYGAPGATGNDRITRHF
                     IENNPIIMAQTNSLDSLNAVQWFIDCGNQDPLSGVNKELHNVLSKRNVTHIYHARDGK
                     HEWKFWREGIYQGVRFISEGLRN"
     gene            complement(18911..19630)
                     /locus_tag="PLK75_04735"
     CDS             complement(18911..19630)
                     /locus_tag="PLK75_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY38586.1"
                     /translation="MNILSIIIPLFNESDTIETLLHSVCHVTLIHQLEKEIIVVNDRS
                     TDDSEIKVKQFIANNPSQNIQYHVLERNSGKGAAIRVGINHAKGDLIIIQDADLEYDP
                     AEYNSLIEPVFRGFADVVYGSRFMGSKPHRVLFFWHTIGNRMISFMSNMFSNLNLTDI
                     ETCYKLFRSEIIKSVNLRENRFGFEPEVTAKIARIKGIRIYEVGISYYGRTYGEGKKI
                     KWKDGFRAIWCILKYGMFRCK"
     gene            complement(19695..20165)
                     /locus_tag="PLK75_04740"
     CDS             complement(19695..20165)
                     /locus_tag="PLK75_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOY38587.1"
                     /translation="MRKPVRETVKNIELEKYLGKWFEIARFPHSFEKDLQGVTATYSM
                     GDNGKILVLNEGYKGSLSGEHKIAKGKARVVDAANPGYLQVSFFLWFYADYYIMELDT
                     INYQYSLVGSRSSDYLWILSRTPQMDENTFKMLTDKALNLGYDLSKLQMVEQHK"
     gene            20432..23398
                     /locus_tag="PLK75_04745"
     CDS             20432..23398
                     /locus_tag="PLK75_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY38588.1"
                     /translation="MWKIVWITILTSLCSVVWNATNVYIPVPKQTDQHSVPPFVNAGQ
                     QWVDSVFNAMTEDEKIGQLFMIAAYSNRDSLHVNYVKKMISEYHIGGLIFMQGGPVRQ
                     AALINEFQSLSRVPLLISMDAEWSLSMRLDSTVLYPRQMMLGAIRDENLIYTMGAEFA
                     RQLKRVGVQVSFSPVLDVNNNPQNPVINTRSFGEDPERVSHLGWKYAKGLQDNHVLAV
                     GKHFPGHGDTKTDSHKDLPVIQHSRERLDSIEFVPFSYCIRKGMGGIMIAHLFVPALD
                     STAMLPTTLSQPVVSGLLRKEMGFQGLVFTDALNMQGVAKYYQQGEVELKALQAGNDV
                     LLFSGDIPKAIEMIKAAVLDGRLKQEQIDESCKRILQAKYWAGLSNFVPIDIQSIVED
                     LNSPKALLLQQQLIESSLTLVRNEKNLIPLIGLDTLRIASVSFGSKQTNSFQNRLGSY
                     SDIASYVYRNDFAGWSGDKIVSYLSKYDIVITSFHNISQLPDKQFGIDTLQVKLIRQL
                     SEQTNVMLDLFGTPYALSLFDLCDSISAIVVSYNDWDITQDLSAQLFMGGIASDACLP
                     VAAGKNFVAGAGATTPKIRLKYSRVPEDAGINSEMIATFDSIALSGITNGAYPGCQVY
                     GARNGIVFYNKSFGKQTYEGNTSVKNSDVYDLASVTKVMATTLSLMKLYDQKKFDVGE
                     KLSKYHSALDTTNKRDITFIDVLSHQACLVPWIPFYANGIKTDSLRRIHYSDTYSEEY
                     KIHVAENMYMRNDFRDSLLARIYDSELLPQKKYKYSDLGMIIMGEIIREISGKTLDLY
                     AIENFYKPLGANRTFFNPLKFLLMNEIIPTENDKTFRKQQLQGYVHDQASAMLGGVAG
                     HAGLFSNANDLGKILQMLLQGGTYGAVRYFSEETVKYFTSAHFLANGNRRGLGFDKQV
                     VSDPGTGPVCASASANSFGHSGFTGTYVWVDPDENLVFVFLCNRVYPVAENNKILSLN
                     IRTNLHQAMYDAIIKD"
     gene            23410..23979
                     /locus_tag="PLK75_04750"
     CDS             23410..23979
                     /locus_tag="PLK75_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38589.1"
                     /translation="MKKAKFTLCLLCMIFLLMAKHSEAQYLENSPGPWQRIFYGGDMG
                     LSFGRITLINISPQVGYRITNRWSAGIGGTYIYYKDNDFNFHMNIWGIQEFMSFTLIK
                     DLSNILPFGNEIGGLLVYGEMNHMYLYRSISNPPGADRYWITSPLLGPAYQLRVGNRS
                     YLLIMLLYNFNESAESPLTNPVFRVSLQF"
     gene            24049..24486
                     /locus_tag="PLK75_04755"
     CDS             24049..24486
                     /locus_tag="PLK75_04755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY38590.1"
                     /translation="MKIKKDSTVKLTYTLYFDNFDGEVFETIDEKEAMEFTMGKGEML
                     EEFEKRIIGMQPGDDFKMMIPMADAYGEESEEAIMEFPLNMFKDVPREELEPGNIVPM
                     EDEDGSEFEALVLELNEDNIVLDFNHPLAGEDIYLTGKILDVK"
     gene            24523..25266
                     /locus_tag="PLK75_04760"
     CDS             24523..25266
                     /locus_tag="PLK75_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38591.1"
                     /translation="MQYSGDDELYNKLMELVNNDPTKFYIIESQVDAEIQLKYFEYSA
                     RLRKEENRTPQELIDDFKDAEDDEIKKEFLIRLALTGNVEAYRFIESYVPEAPRELQP
                     WAMLAYQEARLLLETSLLEESRVFISSGMGGKGHKLRYAVVMISSNKNNLLDFQKDLV
                     AEELKHFFIRKDCELEEIEFNRQFAIITCLIPISIQLQLLFDEIIGEINVMGNFLSET
                     YLITNEKKLNDNDLQKFSDSVAIRESTQE"
     gene            complement(25329..26486)
                     /locus_tag="PLK75_04765"
     CDS             complement(25329..26486)
                     /locus_tag="PLK75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38592.1"
                     /translation="MKKINFFFIFTFAFLTAFGQVPMSFKYQAVIRDAEGHPFDNQNL
                     DVRISIIAGEIGGTAVCQETFDTETNAFGLVTLEIGSQNPTDFSQIDWTEGPYFVKIE
                     IDLGDGFTEFGTSQLLAVPFAIHSKTAESLTGEITETDPIFSGWDKDYNDLTNTPILP
                     VVPENVSEFNNDAGYLTEDTETDPVFGASVANGITAIDTAHWNALESSHWASTGDNIN
                     NMNSGNVGVGIVTSLSFEKDKTFKEIKLTDDEIKQLIIDIAETNALLQFSGKDYYAKV
                     ADASYSGLTINGNELWYISFHNKPENTEAREIINKHFSIDIINNNRQIITRDSKGKPV
                     DYGIIISKAHPNICALRKNLKGGYVNSAANSCLVAFILLAMIALICWNIFA"
BASE COUNT         7743 a         5100 c         5504 g         8206 t
ORIGIN      
        1 gctttattag tcaggaaagt atggatgcgc aacaagcagc tcttgttaaa aagcaggaaa
       61 gcctgcaggc tcttgatgaa cagcttactc aggaattgct tgatagacag aaagatatga
      121 ataagcaatt gtacgatacg gtaatgaatt atattaaaga atttaacgaa gggcagtatt
      181 gtattatttt gggaaatgcc tcaggaagca atgtacttta tgctcaggaa ggcatggata
      241 ttacccgtga aataattgat aacctgaacg atagatattc aaaatcgaaa aaataatgta
      301 gtaaccctat gggatttgcc aatcattatt ttgaaagatt tggtgtgtcc ggaactttat
      361 tatctgctcc tgttgcgata acgccagata tcattgttgt tataccggta tgtgatgagc
      421 tgggtttgcc cgattgcctg gattcgcttg cttcctgcac cgggcatagc tcaaagattc
      481 ttgtgatttt tgtcgttaat catggaatac atgcttcaga tgcagtaaaa aatcaaaatg
      541 ccgaaacata ccgctatatt gaagaatgta aaaactcaat gtgccgtgaa gactttgcca
      601 ttcatgaatt aatgcttggt aatgtagctc aaaaaacagc aggagtaggg ttcgcgcgta
      661 aaacagggat ggacgaagca attcgtgttt ttaacatgtt gaataaatca gatggtatta
      721 ttgcttctct tgacgcagac tgtaaaattg aaacgaatta tttctctgca ttagaagagt
      781 ttttcaagaa aaattcaaac tctggcgctg cagtgatacg atttgcgcat ccattatcgg
      841 gtaatgagta ccccccagaa atatatgatt ccgtctgtct ctacgaattg tatctgaggt
      901 attttaaaca tgccctcagc tattgtggtt atccgttccc tatttacacc atcggctcct
      961 gcttcgcagt agatgcaaaa gcatacattc ggtctggtgg aatgaacaaa cgtcaggggg
     1021 gcgaagattt ttatttttta cataagctta ctgcttttgc gaatgtggga tatatatcgg
     1081 gcacctgcgt gcagccctcg cctcgaatat cgatgcgtgt accttttggt acggggccag
     1141 caattcaaaa aattctaaat accggggaat ggaaagtgta taactttgca ttattccatc
     1201 aactgaaaca attttttgca atttttaaat tgttttatta tatggacgaa aatgctgttt
     1261 acgaaaaagt ggacagtatt aaccaacaac tcggaaattt ctgtcgaagt gttgatctgg
     1321 cggcgaagat acaatcaatt aaattgaaca cagcttcttc ggtttcgttc gaaaagaaaa
     1381 tgtttcagtt atggaatgtt ttcactatta ttaagtacct gaattctcaa agccaagggc
     1441 aattcccttt acaaaaaaca gaaaatgctg catatgaact tttgactgca ttgaattgta
     1501 gtgttaaaac caatcatcct aagaatttac tggagatttt tagggctatt gacgaatctg
     1561 tttgaacaaa aagtataaga atctgtttaa aattattaat attgatgcat acttattgag
     1621 tctatgaaaa aacttatagt tgttgtttta ttcattcttc aaatcactgc ggtttggtcg
     1681 caaaaaggag ctatttccgg aagaattttc gatgaatcga ataatactgc gctccctttc
     1741 agcaatatat acatttcggg gagtcagatt ggtgcaacct ccgatttcaa cgggaatttt
     1801 cttattaccg cactggagcc gggtatttat cagcttaccg cgcaatcact gggctatgcg
     1861 acggtgttta ccgaagaaat cagagttatg gcaggaaaaa ctgccaatat caatattggc
     1921 atgcgtgcag aagcgcttca actcaaagaa gttgaagtaa aggcaaacgt atttcgaaag
     1981 gttgaagagt cccctgtttc gctgcgtact atcggggttt ccgaaattga aaataatccg
     2041 ggggccaacc gtgatatttc gaaagtaatt caaactcttc caggagtagc ggctttcccc
     2101 ggacaaaacc gcaacgacat tgtagtgagg ggggggcttc gaacgaaagc cggttttatc
     2161 tagatgatgt cgaaattcca aacatcaatc actttgccac gcagggtgca tcagggggta
     2221 ctaacggaat tattaatgcc gattttataa gaggtgtcga tttctattcc ggagcctttc
     2281 ctgcaaatcg gggcaatgca ttaagcggag ttttcagttt taaacaaatt gatggtaaca
     2341 aagaagcttt gcgcttcagg ggaagcctcg gtgcttctga agttgctctc agccttgatg
     2401 gtcctttggg tgaaaataca acctatctga tttcggcgcg aaggtcgtat ctgaattttt
     2461 tattcaaact tattggttta ccattcttgc ctactttcaa cgatatgcaa ttcaaagtga
     2521 aaacccgtat cgacgataag aatgagatta gttttattgg actcgcatcg tatgatgtga
     2581 atgtgctcga caaaaacatt gatgacccta cggaatacca gcgttatctg cttggtaaca
     2641 tttcggaaag caaccagtgg acgtatgcat tgggtgctgt ttataaacat taccgcgaaa
     2701 atggatttca aactatcgta cttagccgca atatgctgta taatcgctat tataaatatc
     2761 tgaataacat tgttgatgat gcagggctga tatctgacta caactctttt gaagcggaaa
     2821 ataaatttcg tttcgataat tttagtcaaa cgaataatgg gttccgcttt aattatggcg
     2881 taaatgccga atatgttaag tacaataata atacattccg caaatactac cggttcgata
     2941 tgctcgacac tttgctttac gccagcaatc ttgggttttt taaatatggt atttcgggtc
     3001 aggtgagtaa atcgcttttt ggtaaccgac tgactttaag tttgggtgtg agggctgatg
     3061 ccaacaccta ttcaaaagaa atgagcaatc ctctgcctca gttgtcgcca aggttttcgg
     3121 tgaattttct gttgaccgac aagttgagcc tgaatgcaaa cgtcggcaga tattaccagc
     3181 ttcctcctta caccagcatg ggatatcgcg atgcttcagg agaactgatt aacaaaaaca
     3241 acaacctgaa atacatacgt gccgatcata ttattggtgg aatcgaatac cgaccgacgc
     3301 aaaccagcat tgtttcggta gaagggtttc ataaggttta tgccaattac cccatgtcgc
     3361 tcaccgattc tatggcactg gcttttatgc cggtagattt cggtattgtt ggcgatggac
     3421 ctgtcagctc tgttgccgaa ggtcgtgctt atggttttga ggtactcaca caaaacagat
     3481 ttggaaataa tatcactctt gttgcttcgt atacgttttc tgtaagcgaa tttaaggata
     3541 aaagcggaag catgatccct tcgtcatggg acaaccgcca tgttttcagt ttgattatta
     3601 caaagcagtt caagaaaaac tggagtgccg gtttaaagtg gcgctttgca ggtggattgc
     3661 cctatactcc atacgatttg gagacatccg caatacgctc tgcatgggat attgccggaa
     3721 ggccatattt cgattatgcc agactcaatg gcgaaaggtt tggagcattt catcagttgg
     3781 atattcgtat tgataaaggc tggaatttta aacgaagctc acttaaacta tatatcgaca
     3841 ttcagaacct ctataacttc aaatcggaaa cccaggatat ttttgtgaat accgatataa
     3901 atggaaaccc agtgatcaat ccgctcgaca attcaaaata tgtattgcgt gcactcgaca
     3961 aggagggtag cggcacggtg gtgcctacga ttggtataat tattgatttt taaaacctat
     4021 gactatgaaa catataatta ttgcagcagt ggttgcaata ttcgcaacat tcgctcaggc
     4081 tcaggatacg ccaaagaaat tgtgcaaaaa aaagcagcat gaacctgaaa atattgtact
     4141 gaatagtcag ggagctggca ataactttat atttactttt actccgggga aataccataa
     4201 ttaccctact ttggcaatat ggattgaaga cacagaaggt aactatattc aggatttatt
     4261 tgttacccaa tcgtttgcta aaggcatatt tcgttatggt gcccagcaaa acggaaaatg
     4321 gacagagggt ccacgaaggt atcaggctac cctgccatat tatctgcata aaagggcaac
     4381 agcactccat ctcaccgata ttgtccccga cagtaagcag cctgtacccg atgcatacac
     4441 cggtgcaact cccggcgcag acttcagtct taaggttaat tccaacgaga ttatgaaaga
     4501 aaaataccgc atactcattg agattaatca ggcctgggac gtgaatgaat actggtataa
     4561 caaccgtttt gaaaacgaca atgattataa agccagttgc caaccatcat tagtgtatgc
     4621 tgttactatt gatccgtctg atttaatgga tgaatacgtt tttaatccaa tcgggcatgg
     4681 tcattattcg ggtaaagacg gaaggcttta taccgacctg agcacattaa caacagcact
     4741 gaaaatattt actaaaataa gtgttgttgt tgcgccctga cgcaacacct tttgtactat
     4801 tctgtacttg atttactgca atatcgcatg tatatttatc gttatttatt caaataatat
     4861 tgagtaattt ttaatgataa gcctatgaaa aatattcatt tgctattgct tatttctgcg
     4921 ttgattactt cgagttgcgt ttactctgct gagctgaaga aaaaacctgc cggcgacacg
     4981 gtaacttgtc gcgaaatcga aaagtcggta gttcttatat caggaaacct gtacgcaggt
     5041 aaatttgaag tatcgaataa attgtatagg atatttctta caaatcttat gaaaataggt
     5101 gctgatgaaa aatattcgat tgctcagatc gactctctta attggcgcga aacccgttgc
     5161 ggctttgctg aattatttct tgaaccttat gtagaacttt atttcagaca cccagcttac
     5221 tcagagcatc cggtagtaaa tgttagtttt caaggcgccc aattgttctg tgaatggctt
     5281 accggttatt acaattccat gcccgggcga aaatataaaa aagtgaggtt ccgcctcccc
     5341 actgatacgg aatgggagca tgcagcaaaa ggcggtttgg aatacgtatt atatccttgg
     5401 gggaaccgac ttatttataa taaccagtat gcctgcaatt attaccatgt gggtgatgaa
     5461 aatataatac gaaatatttc aaccggcact ctaatagtaa atgagcgcaa agccgatgca
     5521 agtcctggaa aacttaacga tttcaatagc cttacagtac ctgttaccta ttaccagcct
     5581 aatgagtttg ggttgtataa tgtgtgcggt aatgttgccg aaatggttat tgataaaccc
     5641 gtagcaagag gtggaggctg gttaagcccg ggtggtgatg ttaccatatt ttcctcggca
     5701 gtatgcaccc aatctgctat tgatacaggt tttcgatatt ttatggaagt ggttgaatat
     5761 taatatgttg ggataaattt acctcgcttt gatttcggcg ggcttgtttg cagaataaaa
     5821 atacttggta ttatcgtatc aaaatgatat caaagacgct ttgggcctct ttgattttat
     5881 ttgaattgat tatttttgaa aaaaataact ccgcaattat gaaacgtaca ttcatctttt
     5941 taaacttatt gattgtttta acgctgggtg tcaatgctca aaagataaac ttttatgtta
     6001 ctggtggata tacatttaca agagcccctt tgaaagatgt ccatgaattt atcgggctat
     6061 ataacgaaaa tccgaataaa attcatggat atgtttttac tcacggcttt ggaagtttaa
     6121 aatgcctgaa cggtatatat ttgggcggag gcatgaattt taaaggcatt ttcctggcag
     6181 aactgaatta cacgcaaaag tatcgtcaca cttttgccta ttacgacccg gcaaacaatc
     6241 ctgattatct gagagttgag ctgggctaca gcatttcaac cgttgaagca gcctttttag
     6301 ttccttacag tttcggaaaa ctcactgttg cgcccggaat aggaatggga ttcttatctc
     6361 gtaaaatgta ttactgggat gacgactcat taatgatagc accaaaacgg agagatatga
     6421 gcgtagaatc gcgtaggggg tcattctccc ttgacccaat gcttcaggta agttataagc
     6481 cgttccggaa aattcctgtt gagtttgtag cacgcactta ttatcaggct atgttttcaa
     6541 agatgtcagt tagctggctg ggagattttg aaggctactg gagtccggct gataccgcat
     6601 cgaaaaaggt tacaggagga aatctgggaa ttattttggc tataaaagta aatataccgg
     6661 aaatcagctt caaacatcgg tcaaagaatg tagaacccgt ggtgatagaa ccggtttatg
     6721 aggaaaaaac gaattttgtt ttgtcaggta ctgttcagga tgcgaaaaca agtttgcctt
     6781 tgaatgcagt tattacactg tatcagggaa caaatcctat agaatcggtt atttcgcaga
     6841 gtggaaagtt cacgttaaaa cctgctttaa acagtgacta cacgatcgaa accaaagcat
     6901 ttggatacca aaccaaaacc gaaaaactgc gtttcgacag taaatcaccc aatccttata
     6961 aatataatgt gaaactttcg aaaatacctg cagggcaggc cattgtgctc gaaaatattc
     7021 ttttcgaaaa atcatcggca gtattacttc ctgaatcgta caccgaactg aacaagctgt
     7081 tgcagttcat gaacgatgac tctgaaatta gaattgagat tgcagggcat acaagtagtg
     7141 atggtgacga tgcgtataat atgaaattgt ctcaggatcg tgccaattca gttgcgacat
     7201 atttaattaa caagggaatc gataaaacac gtattgtagc taaaggctat ggagaaacta
     7261 tgcctgttgc ctcgaacgac accgaggaag gcagaagatt gaaccgcaga gttgaattta
     7321 aaattgtcaa atagtatgaa gtacttattc cataaattct cagtattcct caccggaata
     7381 ttgattttcg ttatagcgca gtgtcaggct cagacaatac aggtaactta cgctgtgatt
     7441 ggaagcgata ctctgcacag ctggttcagc gatacgtgca ttgtgatgga gaaattcagt
     7501 atgccttcaa tgcgttatgt tgataaaatc gacttcaaga aaaaggaagt tattgtgcaa
     7561 ataaaccagt ataacgattt tactagaaac acacacaatt caatatttac attcaaagaa
     7621 cttaaaaaga agaaaccttt tatgcccatg ctggtgaagc ttaccgatga aactaaagtt
     7681 atactgggtt tcgagtgcca taaagcattg gtagaaaaag agcagactca ttatgatatt
     7741 tggtacacca aactccctca attacctgac ctgaattata acttctacag catttattac
     7801 caaattcccg gtgttataat tgaggagtgg atgaatggta atctaatcat gcacgctgtt
     7861 tcggttgtgg aaggaaagtg gtgtaattat gaatgggtcg attatcaggg gttttagcat
     7921 tctaatgagt tacagtctgt tttaaaatag cgtgaaagca aatggtaatt catatggttc
     7981 tgaaaaatat tttatgtgta tatttgagtc aatcctttga taagaaaaaa gagggattca
     8041 aataacaaaa caattaaata tgaataactt cacctttcag aacactacac gcattcactt
     8101 tggcaaaggg cagatttcga aactggatcg agaactgtca ccctacacgc gcataatgct
     8161 tacctatggt ggtggaagca ttatgggaaa tggcatttat gagcaggtga aatcactgct
     8221 taaaggtaaa aatgtaatag aatttggtgg tattgaacca aatccccgct acgaaacttt
     8281 gatgcgcgca gtggaaatgg ccaaaatgca gggagttgag tttcttcttg cagtaggagg
     8341 aggatcggtt attgatggca cgaagtttat tgcggctgct gttccattta ctgatggcga
     8401 cccctggcgt atgcttacgg aaggtgctaa aatttaccat gcactgccac tgggaacagt
     8461 tcttaccctg cctgcaaccg gctccgaaat gaatgggaat gcagttatca cacgcgattc
     8521 cgtaaaacag aagctggctt ttggaaaccg gctactccag cctgtattct cggtactcga
     8581 tccggaaact atgttcagtc tgccagatag gcaaatcagt aatggaattg ccgatgcctt
     8641 tgtgcatgta atggagcaat acctaaccta tcctgtaaat tctccggttc aggatcggtt
     8701 ttccgaagca ttgctgataa ccctcatgga ggaggggcca aaggtgctga aaaataaaac
     8761 cgattacgat gcgtgtgcta acctgatgtg gtccgctaca atggcgctaa acggcattat
     8821 tgttgccggc gttcccgacg attggtcgac ccacatgatt gggcatgaac ttacagcact
     8881 tcacggaatc gatcacgcac agagcttgtg tgttgttttg ccgggattaa tgcaacatca
     8941 gagagtgcaa aaagcgcaga agatcattca gtatgctaat agagtgctga aaatcaatta
     9001 cgacaataat gaaaatacca tcgacgaggc tattcgtaaa actgaagctt tttttcttaa
     9061 gattggtatt ccaacaaatc tgggccatta tgatattact gcgaaaaatg ttgattacct
     9121 gtgcgataga ctttctgaaa accgccaaaa tgcctggggt gaacataagg atattgatgc
     9181 tgaggcagtc cggaaaattt tattttcccg gttgtgattt cattaaaata aataacttta
     9241 tacccaaaat tcaattgcta tgaaaaaaca tttactactt cttccgatgc ttcttatttg
     9301 cttttcagtt tttgctcaac tgaatacaag aaccctgacg tgggatggta cagccaggca
     9361 atatctcgaa tatgtgcctt cggtgtattc cggtgaaact ccggttccgg ttgttttttg
     9421 tcttcacggt cttggcgaca atatgacaaa cttcagccag attggtttcg ataatattgc
     9481 agatactgct aattttattg ttattactcc tcaggctctt gtagcatatt atatgagcta
     9541 tgagatcggt acttcctgga attccggagc cagtctgtat ggtattgtgc ccaatgccga
     9601 tgttgacgat tccggttttc ttatggcaat acttgactcc cttcagaacc actataatat
     9661 tgaccctgag cgggtttttg ttatgggttt cagtatggga gggtatatga ccaaccgact
     9721 gggcagtgag catggcgacc ggattaaagc cattgcatcg gttagtggta cttttggaat
     9781 tgccttcaca cctgaccccg tggggccaat ttcttctttg catttccatg gaacagccga
     9841 tgaaactgtg ccatacctca ataatacttc gggaatggat gccgaagagc ttgttgcata
     9901 ttgggtagat attaacaacg catcacaaac tccggaagtt tataattacc ccaatacgaa
     9961 accggatggt ttgacatttg aaagatatac gtatactggg ggagataacg atacttatga
    10021 ttgctttatt aaagttgtcg gtggagaaca taactggtat tattacccga acaacgacat
    10081 tgattattcg attgaaatat ggcggttctt tacaaattcg atgataacaa ccggaatagc
    10141 atcggttgag cattgcgaat caacattata ccccaaccct gcaaccgatt tcattaattt
    10201 tgatattgcc cctgattcga atggaatcaa gtcgttgcaa atttgtgatg ttacgggcag
    10261 agttttatgt caatgcaagg ctaatgttgg ttccaatcat ctagatgttt cgtcattacc
    10321 gtctggagtt tacctactga ttgtaaatga caatgagtcg ttaaaacaat ttcggttcgt
    10381 gaagaaataa aactcgggct tactttcttt gaagaaatgc aattactgca ttggtgactt
    10441 cagcaatggc atcattcagt acatcgttta ttacaatcag gtcgaaacgt tctgcatatc
    10501 gaatttcaaa attcgatttt ttaactcttt ttgcaatatc ttcatcttct tcggtagagc
    10561 gaagcctgag acgccgttcc aattcttcaa tcgaaggtgg cataatgaat attgccagcg
    10621 catcttcctg aaaatactct tttaaattca gcccgccgac cacatcaaca tcaaaaataa
    10681 gattgttccc ctttgcgaaa accctgtcca cttccgaacg caatgtgcca tagtaatgat
    10741 cattatacac ttcctgccat tcgagaaatt cactgttttc gattttcgaa cggaattgtt
    10801 caacgctcaa aaaatagtag tccttcccct cagtctcctg atcgcgcttg ggtctgctgc
    10861 atgccgaaac cgagaactcc aggttcagca ttttattgtt cagcaaatta tggacaatag
    10921 tagtttttcc agaccctgag ggagcggaaa caattatggc cttattgctc tgcattcatt
    10981 taaggttttt gcaaatatac aataacatta ttatgtggat tgtaaaaata atattttggt
    11041 ttttatgttt tcaaaacttt gtagtttaaa ataataatct tatttttcat aacttaaaat
    11101 taatttaata attaattgaa agataggtgg ttagatgaaa cttgtaaaaa caaattaatt
    11161 aaaaagatat gcaaaaacag gtgttacttg gagatgaagc gattgcgcaa ggggcaatcg
    11221 atggaggtgt ttcgggtttt tatgcctacc ccggaactcc gtccaccgaa ataatggaat
    11281 ttgtacaggc ttcaaaagaa gctgcttcta aaggtattca ttgtatctgg tcggcaaatg
    11341 aaaaaactgc tgttgaatca gccatcggaa tgtcgtatgc aggcaaaaga tcaatggctc
    11401 aaatgaagca tgttggtgtt aatgttgctg ccgatgcatt tatcaatgct tcaattacag
    11461 gggtgaatgg cggaatgata ttggttgccg ccgatgaccc ttcgatgcat tcatcacaga
    11521 acgaacagga ttcgcgtttt tatgccgatt ttgctatgat tccgatactg gaacccagca
    11581 atcaacagga agcttacgat atggcctact caggttttga actttcagaa gcattcaatg
    11641 tgccggttat gatgcgtatt actactcgtc tggcacactc ccggtcggat gtagagcgca
    11701 gagcagttaa gccaatgctt aatgggtcat tgccaaaaga cccgcgccag tttgtattgc
    11761 tgccggcaat agcacgtaag aattacaaga aattactggg aatgcaacag gcaatgcagg
    11821 aagcatcgga caattcggac tataacagat atttcgaagg aaccgacaca tcgatgggta
    11881 tagttgcctg tggattggcc tacaattatt taatggaaaa ttttccggaa ggttgtaagt
    11941 ttcccgtggt aaaaatttca caatacccaa ttccgaaaaa tcatttgaca cggttatata
    12001 aaacctgtaa atcgatttta gtattggagg atgggtaccc gttggtagag aaaatgctgc
    12061 gaggattttt cgaatcatca gttcctatta aagggaggct cgaaggcagc cttccgcgtg
    12121 atggagaatt gaatccgaat attgttgctg cggcattgaa tattgctatt caggaaggtc
    12181 aggctgttcc cgatgtagtg aaaaacaggc caccttcatt atgcccggga tgtggtcatc
    12241 gcgatatgta caacgcttta aacgaagcca ttgcccctta tgggacaggc cgggtttttt
    12301 ctgatattgg atgctacaca ttgggagcac tgccgccatt caatgctatt aattcatgtg
    12361 tggatatggg ggcttcaatt acaatggcaa taggagcagc cgatgcaggt ctggttcctg
    12421 ccgttgctgt tatcggggat tctactttta cacactccgg gatgactgga ttactcgatg
    12481 ccattataaa ggattcaccc attcttgtta tcatttccga taactcgaca actggtatga
    12541 ctgggggtca aaattcgcat ggtgttggaa gaatatataa tatctgtaag ggtctggggg
    12601 ttagcgagga acatttgcat gttgtaaccc cgctccgtaa ataccatgag gaaaatacgg
    12661 aacttttcag gaaagagctt gctcacaaag gcttgtccgt aattattcca caacgggaat
    12721 gcattcaaac cgccgctcgg cacaaaagag aaacttcgaa aaatagtaat taataaaatc
    12781 cgaaaaatga aaacagatat aatattggct ggtgtaggag gtcagggaat tctaaccatt
    12841 gctgcaatta taggagttgc cgcggtaaat aaaaaaatgt atctcaagca gtcggaagtg
    12901 catggtatga gccaaagggg aggcgacgtt caatcaaatc tcaggatctc tgagaaaccg
    12961 atttactctg atttaattcc caagggtcag gctgatctga tattatcggt tgaacctatg
    13021 gaagccctgc ggtatttgcc ctatttgagt aaaagcggct atctcattac aaaccttgta
    13081 acattcaaca atataaccga ctatccggaa gaagcccgga tttttgcaga aattgaaaaa
    13141 ctgcccagga aagttgcaat tcaagccgat ctgattgcta aagaaattgg ttctgcaaga
    13201 gctagcaata ttgtgatgct gggcgcggcc agctcgtttt taaaccttag cattgaagac
    13261 ctggaatacg gtattacttc acttttcagc aagaaaggag aagaagtggt tgcgatgaat
    13321 attaaagcac tgcatgcggg acgtgatgcg gcttcgcagt acctaaaaaa gtagatatta
    13381 atacaagttt attttcaact gccgcagata ctcatgtgtc tgcggtttga ttgactttaa
    13441 ataatgactt tggggttgtt taattaccac cagcagattg ctaaaagaaa agaggctgtc
    13501 tacatgtaaa cagcctcttt taaatattca atctggaaaa gcagtgttac ctgcttgtca
    13561 tactaaaaat ccatttcttc gagtttgtcg aaaccttcgc actcatacaa tttaaaagaa
    13621 acttccatta aactggtgag cattttttca ttttcaggct cttttgccat ttcttcgaat
    13681 attttttcgg cttctttgtc atccttgtat ttgtcttcga atttttttga aacatcactc
    13741 atttctttca taagcttatt cagatcagca gcttcggcat cgtcaatctt tttgtcttca
    13801 acaaccttgg ttgcagtttc ggtgtatttt ttcatcatac cgaacatttt ttttgcatcg
    13861 gctttggggc caccgcatga aacgagtgca aatacgaatg caactaatcc taaaagtact
    13921 gtgatttttt tcataatagt ttgttttagt tagtgtttaa tttattaggt aaatatcgtt
    13981 ttttatttta ttcgaaacaa tagcaaaccg aaaaaaaaaa gcttttcttc aaaaaaaaca
    14041 tgcttttatt gtttaatcat tgaagattct ttaatttatc gctgccatca caggaaaaca
    14101 gcctgataac aagagtattc atctcatcgt aaatggcttt gttttcatcc ttatttatcg
    14161 ttttttcgaa agcttctgtg tcttcaccat aagtattcaa ttcagccagg agcgcatcaa
    14221 gtttctgaac ttcggtatca tcgagcaccg aatcctgaac agcgttagtg gcaacggaat
    14281 taaattcttt aattttatta acgacagcag tgctgttatc ggagcaggaa acaaagaatg
    14341 taactcccaa aataattgtt gctattgtta gtactgtgtt tttcatataa tcattaaata
    14401 aaaaacgcac cgaagtgcgt ttaagttatt ttttactttt agcttctttc acaatggcat
    14461 cgaaagcttt gggctgattc attgctaaat cggccagcac tttacggttg attatgatgt
    14521 ttttatgatg cagcagaccc attagctgtg aataactcag gtcgtgcatt cttgcaccgg
    14581 cattgatacg ttgaatccat aaggctctga attctctttt cttggctctg cggtcacgat
    14641 atgcatattg caaacctttt tcaagggtgt tttttgcaac cgtccaaaca ttttttcttc
    14701 tgccaaagta tcctttggtc agcttcatta gtttttttct gcgttctctt gcagcaactt
    14761 tgtttacact tcttggcatt tttttctttt tttatgactg tcagcgtttc tctttacaga
    14821 aatctttcgg ctgcgcaatc tggttaaaca cttaattgat taattacttc atgcaaagca
    14881 tttgtttcac atttttctcc actgtgctgt cgactagggt aaaataacct aaatttcttt
    14941 tacgtttttt cgactttttg gtgagaatgt gactcttgta agcatgcttt ctcttaattt
    15001 ttcctgttcc ggtgagctga aatctttttt tggcgccgga gtttgttttc atttttggca
    15061 tcgctttata gtttaaatta tcgcgtttta ctttttcttt ttacctggtt taggattaat
    15121 tataaggaac atctttttcc cttccagttt tggcaagctt tcaactttgc caaattcttc
    15181 cacatcgttg gccagacgaa gcaaaagtat ttcgccttgc tcctggaaaa gaatggatcg
    15241 tcctttgaag aaaacaaaag cttttacttt tgatccctct tcaagaaacc gctgtgcatg
    15301 tttcaatttg aactgatagt cgtgctcatc ggtgttcggg ccaaaacgaa tttctttaat
    15361 aactactttc tgagttttag ctttgttttc tttcagcttc tttttctgct gataaagaaa
    15421 cttttgatag tcgattaccc tgcaaaccgg tggttctgct tttggtgata tctccaccag
    15481 atcaagatcc aaactttggg caacctgtaa tgcttcacgc agactaagaa tctgagtttc
    15541 tacattttca ccaaccagac gaacaaaggg tgcagtaatg taatgattta cacgaaattg
    15601 gttatcatcg gcttttttaa aaactctgcc ggtatttcta ttctcagcta tagcttaaac
    15661 ctcctatact tttagttaat attgtttatt taataaattc aataatttcg tttcgtatca
    15721 tttcggcaaa actttcgggg ctcatacttc ctgcatcacc aactccctgt ttacgcacgg
    15781 aaattgtgcc tgtcgattct tctttctcac caactacaag cagatagggg attctcttca
    15841 gttcattatc acgtatcttc ctacctatct tctcattcct ttcgtcaatg aggctgcgaa
    15901 tatcgtaatt ttttaataat tctaaaactt tttttgcata atctgtgaat ttttcactta
    15961 tcggaagtat tacagcctgt tgcggagtta accagagcgg gaatttgccg gctgtgtttt
    16021 cgatgagaac ggcgacaaaa cgctccatgg agccaaatat tgcccgatgt agcataatcg
    16081 gacggtgttt ttcgttgtcg gcacctttgt aatacaggtc gaaacgctca ggtaggttgt
    16141 aatccacctg aatggttcct agttgccact ttctcccaat ggcatctttt acaatgaaat
    16201 ccagttttgg tccgtaaaaa gcagcttccc ccggggcaat taccggatcg agacccattt
    16261 cgtatgcagc ctcctgaatg gcttgttcgg ctttattcca attttcatca tcaccgatat
    16321 atttggtttt gtcgtttgga tcgcgtagag aaatttgtga aacaaaatcg ttgaacccta
    16381 gtgaacggaa taagaaaaga acgaggtcaa tgatgctttt aaattcatct ttcacctgat
    16441 cctgacggca gaaaatatgg gcgtcgtcct gcgtgaaact ccttacccgg gttagcccat
    16501 gcaactctcc atgttgctcg tagcggtata ctgtaccaaa ttcggcaaga cgaacaggca
    16561 gatctttata tgaaaattgg gtcgatgcaa aaatttcgac atggtgcgga caattcattg
    16621 gtttcagcat gaaggcttcg ccttcgtgtg gtgtttgtat cacctgaaac gaatcttttc
    16681 cgtatttctc gtaatgccct gaggttttat acagttcaac attccctata tgtggggtca
    16741 ttaccagttg atatccacgc cgaataagtt cttttgtaag ccactcctca agtctttttc
    16801 gaagttcagt gcctcgcggt aaccataagg ggagtcccat gcctactttt ggtgaaaaag
    16861 tgaacaactc aagctctttg ccaatgcgcc ggtggtctct tttacgggct tcttcaagaa
    16921 gtacaagata gtcgtcgagt agtttttgtt ttgggaaact tgtgccatat acgcgaacca
    16981 attgttttcg tttctcgtct ccgcgccagt atgcaccggc aatgctcagt atttttacag
    17041 cttttatctt ttcggtgttc ggaatatgcg gccctttgca caaatcaacg aaatcaccgc
    17101 tttcgtacag ggtaatagac ccatcctgaa gttcgttaat aagctccagt ttataggggt
    17161 cgttttttgc tgtgaaaaaa cgaatagcct catccttgct cacgtctctg cgcttatatt
    17221 caattttatg cctggcaatg tcaagcatgg tttgctctat tcttgcaaaa tcggcatcgg
    17281 taagttgagc cccttcgggt aaatcaacat cgtaataaaa tccattttct acacttggac
    17341 caatgccgaa ttttattcca gggtaaagtt tttcgagtgc atgcgccatc aagtgcgacg
    17401 acgaatgcca gaaagcagac tgaccctctt cgctttccca aaggtgaatc ttaacggaag
    17461 catctgtgtg tatgggtctt aataaatccc agacttcgtt gttgacagtt atggcaagtg
    17521 cttcctgagc aagtcggctg ctgatattgg cagctatttc catgcctgtt acaccggctt
    17581 cgaattgacg aacactttta tcgggtaaag taatatgtat cattataaaa aatttgagtg
    17641 caaaattaca aaacttattc ggtaaactca aattctaaaa acgaagtatt acaaactttg
    17701 atgaattgcg tgatgtcttc attaatacaa accggaagac atgaatactt tattttcaca
    17761 tcaggtgttt atattagaat aaacaacccg ttgtgcattt gaaaaataaa taaaaggcct
    17821 ttgttattgg gccaatgaat taatcgaaaa tcgacaaaca taaattttca aaaacaactt
    17881 cttccgtatg ttttacttgc atacttgcat tgtttacaga catgttctat ccgtatgctg
    17941 aactaatttc ttaatccttc acttataaac ctgactccct gataaattcc ttcgcgccaa
    18001 aatttccatt cgtgttttcc atcacgtgca tggtatatgt gagtaacatt tcttttactc
    18061 aaaacattgt gaagttcttt atttacacca ctcaacgggt cctgattccc gcaatcgata
    18121 aaccattgta cggcattcag tgagtctaat gaattggttt gcgccattat aattggattg
    18181 ttttcaataa aatgccgagt aattctgtcg ttacctgttg ctccaggggc tccatagagc
    18241 atcgacatgt atttatataa ctgttcaggc atacttacga ggccgtcttc gctaaatatt
    18301 gaagcactca aagcgacaca atacgaaaat ttatcgggat gcttcattgc ccataccaaa
    18361 gagccatatc cacccattga taaaccggca accgctctgc tctctttatt tggaattgtt
    18421 ttataagtgc tatcaataaa aggcaaaaat tctatgaaca taaaatcttc gaatcgtgct
    18481 ttgctttgat aatcattaat ataccaatag tatttgccgt ccggaattat tataatcatt
    18541 ggctctattt cgccagtagc aataccagtg tccattattt tatttactct gccaaggttt
    18601 atccaagatt tatggttacc tttataacca tgcaataaat ataatacagg atattttttt
    18661 tctgagttat cgtaatcagg cggaagatag attgagtaat gaacgtcgta acctaaaaca
    18721 acacttttat acctcaattc tttgtttact tttccttttg agggttttat ttttgtggtt
    18781 tgcgcaaatg tactattaaa aataaaaaat aatacgaatg ccattgacaa attagttttc
    18841 atgtttctgt aattttacat tacattacat taaatattcc caactttctt caattcaaaa
    18901 ttaaatcaaa tcacttgcat cgaaacattc cgtatttcaa aatgcaccag atagccctga
    18961 agccatcttt ccattttatt tttttccctt caccgtaagt cctgccataa tacgaaatgc
    19021 caacttcata aattctgatg cctttaatac gggctatttt tgccgttact tccggttcaa
    19081 agccaaaccg gttttcgcgc aggtttaccg atttgataat ttcgctgcga aataatttat
    19141 aacaggtttc aatgtcggta aggttgaggt tcgaaaacat attcgacata aaactgatca
    19201 ttctgttgcc gatggtatgc cagaagaata acacacggtg aggtttgctt cccataaatc
    19261 ggctgccgta aacaacgtcg gcaaatcctc tgaaaacagg ctcaatgagc gagttgtact
    19321 cagctggatc gtattcaagg tcagcatcct gaattataat taaatctcct tttgcatggt
    19381 ttattcctac tctgatggcg gcacctttcc ctgaattccg ttcgagtacg tgatattgga
    19441 tgttttgtga cgggttgttt gcaataaatt gcttcacttt gatttcggaa tcatcggttg
    19501 aacgatcgtt tacaacaatg atttcctttt ctagctgatg aataagtgtt acgtggcaaa
    19561 ccgaatgtag aagtgtttcg atggtatccg actcattaaa taagggtata attatcgata
    19621 aaatattcat ctgtacattt tatcacacaa acataatact atttaaagta aaaagtattt
    19681 tgtttcacga aatattactt atgctgctct accatctgga gtttgctgag atcgtaaccc
    19741 agattgaggg ctttatcggt aagcatcttg aatgtgtttt cgtccatctg tggtgtccgc
    19801 gataaaatcc aaaggtaatc tgatgagcgg ctgcccacaa gtgaatattg gtagtttatt
    19861 gtgtcgagtt ccataatata atagtcggca tagaaccata aaaagaatga cacctgcaga
    19921 tatcccggat tggctgcatc tacaaccctt gccttgcctt ttgcaatttt gtgctctccc
    19981 gataaactgc ctttatatcc ttcgttaaga accaatattt tcccattgtc tcccattgaa
    20041 taggttgctg ttacaccctg caaatctttt tcgaaggaat gcggaaatcg ggcaatttcg
    20101 aaccatttcc ccaagtattt ttccaactca atattcttaa cagtttctct gacaggcttt
    20161 ctcatgttta atgaaataaa tgaatatgaa attactgaga ttattatcag taaaaaaatt
    20221 aataggagta gtgttgtttt tttcatgaca taaaagtaat aatttttaac aaggttttta
    20281 aaaatttgtt catgcatgtg ctatcggttg acatttccgt cgctgtaaaa aaagataatc
    20341 cacaatgttt aacgataact tgttaatatt ctgcttgtgg tatataagat taaaattttc
    20401 aattcgttac atttgtgtag tttgtcaaaa tatgtggaaa attgtctgga taacgatttt
    20461 aactagtctg tgcagcgttg tgtggaatgc aaccaacgtg tatattccgg tgccaaaaca
    20521 aacagatcag catagtgtgc caccttttgt taacgcgggg cagcaatggg tagattctgt
    20581 gtttaacgct atgactgaag atgagaaaat cggtcagctc tttatgattg cagcctattc
    20641 gaacagggat agccttcatg tgaattatgt gaagaaaatg atttcggagt atcatattgg
    20701 aggcttaata tttatgcagg gtggtccggt tcggcaggcg gcacttatca atgaatttca
    20761 atctctgtca agagtaccct tgcttatatc tatggatgca gaatggtcgc tttcgatgcg
    20821 ccttgattct acagtcttgt atccgcggca aatgatgctg ggtgcaattc gagacgaaaa
    20881 cctgatatac actatgggcg ctgagtttgc caggcaactg aaaagggtgg gagtgcaggt
    20941 gagtttttca ccggttcttg atgtgaataa caatccacag aatccggtga ttaacacacg
    21001 ttcgtttggc gaagatcctg aaagggtttc gcatttgggt tggaaatatg ccaagggctt
    21061 acaggataat catgtgcttg ccgtgggtaa gcatttcccg gggcatggtg ataccaaaac
    21121 agactctcat aaggatttgc cggttattca acacagtcgt gaaaggctcg attcaatcga
    21181 atttgtgccg ttctcatact gtatccggaa agggatgggc ggtattatga ttgctcacct
    21241 ttttgttcct gcactcgata gtacagctat gttgccgaca acactttcgc aacccgtggt
    21301 ttccggtctg ctccgaaaag aaatggggtt tcagggattg gtttttaccg atgcgttgaa
    21361 tatgcagggt gtggcaaaat attatcagca gggtgaagtt gaattgaaag cattgcaggc
    21421 gggaaacgat gttctcttat tttcgggcga tattcccaaa gctatcgaaa tgattaaggc
    21481 agctgttctc gatggtagac tgaagcagga gcaaatcgac gaaagctgta aacggatatt
    21541 gcaggcaaaa tactgggcag gcctcagtaa ttttgttccg attgatattc aatcgattgt
    21601 tgaagatttg aactccccca aagctctatt gttgcagcag caattgattg agtcgtcgct
    21661 gaccttggtg agaaacgaga aaaacctgat tccgttaata gggctcgata ccttgcgtat
    21721 tgcttcggta agttttggaa gcaagcaaac aaatagtttt cagaatcgac tgggcagcta
    21781 ttctgacatt gcatcatatg tgtatcgcaa cgattttgca ggttggtcgg gcgataaaat
    21841 tgtatcgtat ttgtcgaaat acgatattgt tattaccagc tttcataata tcagtcagtt
    21901 gcccgacaag caatttggta tcgataccct gcaggtgaaa ctaataaggc agctttcgga
    21961 gcagacaaat gtgatgcttg atttgttcgg aactccctat gcattgtcgt tgttcgacct
    22021 ctgcgatagt atttcggcaa tcgtagtgtc gtacaacgat tgggatataa ctcaggatct
    22081 ttcagcccag cttttcatgg gaggtattgc ttccgatgcc tgtttgcctg tggcggcagg
    22141 aaaaaacttt gttgcaggag ccggtgcgac cactcctaag atacgtttga aatactcaag
    22201 ggtacccgaa gatgcaggga tcaattctga aatgatagct actttcgatt ccattgccct
    22261 cagcggaatt actaatggcg catatcccgg ttgtcaggtt tatggagcgc gaaacggtat
    22321 tgtattctat aataaatcgt ttgggaaaca aacttacgaa gggaatacaa gtgttaaaaa
    22381 cagcgatgtt tacgaccttg catcggtgac aaaagtaatg gcaactacgc ttagccttat
    22441 gaaactttat gatcagaaaa agtttgatgt tggcgaaaaa ctttcaaaat accattcagc
    22501 acttgatact acaaataaaa gggatattac gtttattgat gtccttagcc atcaggcgtg
    22561 tttggtgcca tggattcctt tttatgcaaa tggcattaaa accgattcac tccgccgaat
    22621 ccattattcc gatacctatt ccgaagaata taaaattcat gttgccgaaa atatgtatat
    22681 gcgtaatgat tttcgcgact ctttgctggc acggatttat gattcagaat tattgcctca
    22741 gaaaaaatac aagtactccg atctgggaat gataataatg ggtgaaataa tcagagagat
    22801 atcaggtaaa actctggacc tctatgccat tgaaaatttc tataaaccac tgggtgctaa
    22861 ccgtactttc ttcaacccat tgaagttttt actaatgaat gaaatcattc ccaccgaaaa
    22921 cgataaaact ttccgcaagc agcaattgca aggctatgtg cacgatcagg cgtcggctat
    22981 gttgggaggg gttgccggac acgcaggact cttttcgaat gccaacgatc tgggtaaaat
    23041 ccttcaaatg ttgctgcaag ggggaactta tggcgcagta aggtacttca gcgaggaaac
    23101 ggtgaagtat tttacctctg cacattttct tgccaatggc aaccgccgtg ggttgggatt
    23161 tgacaaacag gttgtctctg atccgggaac aggtcctgta tgtgcatcag catcggccaa
    23221 cagttttgga cacagcggtt ttaccggtac ttatgtgtgg gttgaccccg atgaaaatct
    23281 ggttttcgtg ttcctgtgta acagggtgta cccggtggcc gaaaacaata aaatcctatc
    23341 tttaaatatc cgcactaact tgcatcaggc tatgtatgat gccattataa aagattaaaa
    23401 ttgggaagaa tgaaaaaggc gaaatttaca ctctgtctgc tatgcatgat ttttctgtta
    23461 atggcgaagc acagtgaagc acagtatctt gaaaatagtc cggggccgtg gcagcgaata
    23521 ttctatggag gtgatatggg tttgtctttt ggaagaatta cccttattaa catatcgcct
    23581 caggtgggtt atagaattac gaaccgctgg tcggccggaa taggtggaac ctatatttat
    23641 tataaagaca acgactttaa ttttcacatg aatatatggg gcattcagga gtttatgagt
    23701 tttaccctaa taaaggatct ttcgaatatc ctgccttttg gtaacgaaat cggtggcctt
    23761 ttagtttatg gagaaatgaa ccatatgtac ttatatcgtt caatcagcaa tccacccgga
    23821 gccgaccgat actggatcac cagcccttta cttggaccgg cgtatcagtt gcgggtgggc
    23881 aacagatctt atttattgat aatgcttctc tataatttta acgaatcagc cgaatcgccg
    23941 ctgacaaatc cggtgtttag ggtatcgcta cagttttaac cggtagttgt tttgagtttg
    24001 cagaatattt ccatatattt acataattat taattaacac taatttctat gaaaattaaa
    24061 aaggatagca ccgttaaact gacttacacc ttatatttcg ataacttcga cggagaagtg
    24121 tttgaaacaa tcgacgaaaa agaagctatg gaatttacta tgggaaaagg cgagatgctc
    24181 gaagagttcg aaaaaagaat tatcggtatg cagccaggcg acgactttaa aatgatgatt
    24241 ccaatggcag atgcctatgg cgaagaatcg gaagaagcca ttatggagtt cccgttgaat
    24301 atgttcaagg acgtaccaag agaagaactg gaacccggaa atattgttcc gatggaggat
    24361 gaggatggct ctgagtttga agccttggtg ctggaactga acgaagataa tattgtgctc
    24421 gattttaatc acccattagc aggtgaggat atttacctta ccggtaaaat attggatgta
    24481 aaataagcgt tatcagcagg taaaataaca aatactcggt gtatgcaata cagcggagac
    24541 gatgagttat acaacaaact catggagctt gtaaacaacg acccgacaaa attctatatt
    24601 atcgagtcgc aagtggatgc tgaaattcag cttaagtatt tcgaatattc ggcacgtctg
    24661 cgcaaagaag aaaatcgaac tccacaggaa ctcatcgacg acttcaagga tgctgaagat
    24721 gatgaaatta aaaaagagtt tctgatccgt ttggccctca ccggcaatgt tgaggcatac
    24781 cgcttcatag aatcatatgt gccggaagct ccgcgtgaat tgcagccatg ggcaatgttg
    24841 gcctatcagg aagcaagatt attgctcgaa acatcgctgc ttgaagaatc gcgggtcttt
    24901 atttcttcgg gaatgggagg caaggggcac aaacttcgtt atgctgttgt gatgattagt
    24961 tccaataaaa acaatcttct cgattttcag aaagatttag ttgccgagga gcttaagcac
    25021 ttctttatta gaaaagactg cgaacttgag gagatagaat tcaatcgcca atttgctatt
    25081 atcacttgtc tgatacccat tagcatacaa cttcagctgc ttttcgatga gatcattggt
    25141 gaaattaatg ttatgggtaa cttcctcagc gaaacttacc tgatcaccaa tgaaaaaaaa
    25201 ctgaatgaca acgacttgca aaaattttca gatagtgttg cgataagaga aagtacacaa
    25261 gaatagccct tgccatttca ataaaaattc acagtaagtt gactctctaa acatagaata
    25321 gactgtatct aagcaaaaat attccagcag attaaagcaa tcattgccaa caaaataaaa
    25381 gccaccaaac atgaattggc agctgaattc acatacccgc cctttaggtt ttttcgtaaa
    25441 gcacaaatgt tcgggtgagc ttttgaaatg attattccgt aatcaaccgg tttccctttt
    25501 gaatcacggg taataatctg ccggttatta ttaattatat caatggaaaa atgcttgttt
    25561 attatctcac gtgcttcagt gttttcaggt ttattatgaa aactaatgta ccaaagctcg
    25621 ttgccattta ttgtaagccc tgagtagctt gcatctgcaa cttttgcgta gtagtctttg
    25681 ccagaaaatt gcaaaagtgc attggtttcc gcaatgtcga ttataagttg cttaatttca
    25741 tcgtcggtca gttttatttc cttaaaagtc ttgtcttttt cgaaactgag cgatgttact
    25801 atacccacac ctacatttcc cgagttcata ttattgatat tgtctccggt acttgcccaa
    25861 tggctgcttt cgagagcatt ccaatgtgct gtatcaatgg ctgtaattcc gtttgcaact
    25921 gatgccccaa atacagggtc agtttctgtg tcttctgtta gatagcctgc atcgttattg
    25981 aattcgctta cgttttcagg aacaaccggc agtataggag tatttgtaag gtcgttgtag
    26041 tctttatccc atcctgaaaa gatcgggtca gtttcggtga tttctccggt aaggctttca
    26101 gctgttttac tgtgtatggc aaatggaact gccagtaatt gagaagtgcc gaattcggtg
    26161 aaaccatcac ccaagtcgat ttcaatttta acaaaatacg gcccttccgt ccaatcgatt
    26221 tgtgagaaat cagtcggatt ctgactacca atttcaagtg taaccaagcc gaatgcattg
    26281 gtttcggtat cgaatgtttc ctgacatacc gcagttccgc ctatttcacc agcaatgata
    26341 ctgattctga catcaaggtt ttgattgtcg aatggatggc cttcggcatc ccttattaca
    26401 gcctgatact taaatgacat gggaacctgc ccaaatgcag ttaaaaatgc aaatgtgaaa
    26461 ataaagaaaa agttgatttt tttcatggtg tggttatttt gataggtatt ggttgttatt
    26521 ccttaattac tttaaatgtc gatacatatt tgc
//
LOCUS       DAOOQP010000028        26254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2222_length_26254_cov_6.051414, whole genome shotgun sequence.
ACCESSION   DAOOQP010000028 DAOOQP010000000
VERSION     DAOOQP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2222_length_26254_cov_6.051414"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..574)
                     /locus_tag="PLK75_04770"
     CDS             complement(47..574)
                     /locus_tag="PLK75_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOY38593.1"
                     /translation="MELRACDNEAYFITLTVIDWIDVFTRREYMDFIVENLNYCRTEK
                     GLEIYAFVIMSNHIHLIVRSKIEPLGNILRDFKTYTSKELFKMIDKNTRESRRDWMIN
                     AFKNAAFNNPLNKFHQFWQNDNQPILINNHETFLTKQNYIHQNPVRAGLVVNDFEYVY
                     SSASENCPLTIDPFS"
     gene            complement(625..810)
                     /locus_tag="PLK75_04775"
     CDS             complement(625..810)
                     /locus_tag="PLK75_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38594.1"
                     /translation="MQQASTLAAIMCFVSGFKKNIANYANSVNTDCLTEDTKKNFVMQ
                     QASTLAAIMNAFCSENP"
     gene            929..2686
                     /gene="aspS"
                     /locus_tag="PLK75_04780"
     CDS             929..2686
                     /gene="aspS"
                     /locus_tag="PLK75_04780"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOY38595.1"
                     /translation="MYRTHNCGELRMANTGTKVTLCGWVQRKRDKGGMIWIDLRDRYG
                     ITQLVFEQGKTPTDILDMVNETGREFVVRIQGEVIARQAANPNLPTGDIEIRVEHYEI
                     LNPSATPPFTIEEKTDGGDDLRMKYRYLDIRRNSVKDNLLLRHKMAQVTRKYLDNIGF
                     IEVETPYLIKSTPEGARDFVVPSRMNPNQFYALPQSPQTFKQLLMVAGIDKYFQIVRC
                     FRDEDLRADRQPEFTQIDCEMSFVEQEDILQTFEGLVKYMFREVKGIDITENFFRMPY
                     SEAMEKYGSDKPDLRFGMCISEMSHLVKGKGFGVFDDAQYVGGICAKGCAVYTRKQLD
                     ELTEWVKRPQIGMKGLIYCRVNEDGTLRSSVDKFYSEDDLKQWANLLNAQAGDLILVL
                     AGDHEKTLKALGELRLEMGNRMDLRNPQKFAPLWVVDFPLLEWDDETQRFYAKHHPFT
                     SPKPEDIALLETDAGSVRANAYDMVINGVEVGGGSIRIFNRELQQKMFSVLGFTDEEA
                     EHQFGFLLNAFRYGAPPHGGIAFGFDRLCSLFGGSDSIRDYIAFPKNNSGRDVMIDSP
                     AAIKQEQLDELFLDLKKPL"
     gene            2814..4415
                     /locus_tag="PLK75_04785"
     CDS             2814..4415
                     /locus_tag="PLK75_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY38596.1"
                     /translation="MIASRNTIVFMLIIVVGIIYTGCKKIRNPVITTLNPVSVTINSA
                     AIETTVSTDGGPDISERGVCWSIHPTPTVISNKKACGSGSGNFSCTVTNLESNTTYYL
                     KSYAINENGVYYGNEVQFTTYDASDLVMVTMNISFITSHYANAYVNINYTNEPSPTQF
                     GIKWSNSPAMTSCDSIIRNFQIPNNSHFKIEGLQPGTQYYMQAFAINDAGTSTSTAID
                     VTTVSANQAPEIQTHEAYDIMQYGASFPAEIISHGSDTITEYGVYVSTSNQFDDATGN
                     TFKLIMQNSNTYFLTNTLLPETHYYYRAYAINAVGTAYGATKNLTTPDDDQIHFSDGV
                     QYGTVTDIDGNSYRTIQIGNQKWFAENLRTSRYRNGELIPEISEFSDWTNSTQGAWCH
                     YDNESRNEIPYGKLYNWYAVSSNDGLCPAGWHVASHTEWLELPENMETTAGFAGHFMR
                     EQGIHWIQNTACSSNSSGFTALPGGCRTSKDNFDGQRDYAYFWSSTQYNASNAIFWQL
                     YGNSDQFSADNIGKSSGLSVRCIKD"
     gene            4522..6363
                     /locus_tag="PLK75_04790"
     CDS             4522..6363
                     /locus_tag="PLK75_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38597.1"
                     /translation="MKKCLTIIAFCFIVYAVRAQHEGIERHFDEYIFGDFTTRGNYYS
                     SDTLTDSFHLFRNTDNFIYSAGSIGSAHIPMFYYKQYQDADFVFFTPYTPFISTLQGS
                     TYFDTRTPFTRIVFVGGEKQLDNVRLVHTQNIRPWLNFGAEFNSLNNEGHYAMQQTKI
                     QSLNAFVSITKHRYNNHLNFIYNKISHENNGGIESDEIFEAGSQRSENLSVNLFSSTT
                     KLSQIGFSYYHELLIGKKLIDTIYQNNDTIQKISYKSRLIFSQSLSYNRYYRVYHDVP
                     GDFYENIYIDSTETYDSVSLRKFSHSFDAKYLIQGEANSIPHSIAMAGFITDLCWYGN
                     NDFLTENSIYGRWFSDTRRLQWDVTGRFYFTGYYAGDLSSIVQLLYYPDKDKNHMLKF
                     SPGYSLTRPDYFYSHYHANHFMWGTNLPKSSRTNLSLTYEYVKWQLHSSLSYQYLDGF
                     LYFDTDANPKVSSANCQVVSAEIGKKLNVGKFHWYNQLQLQYLSNDSILSLPLLGAYS
                     SLYFQSPLFKKVLILQIGVDMTYFSSAFGYAYMPATGIFYRNTEKKYGNYFVFDAYAS
                     IKVKRFRAFVKASHLNDFFMPSNYYGLLHYPADPFSVNFGISWEFYN"
     gene            6377..7756
                     /locus_tag="PLK75_04795"
     CDS             6377..7756
                     /locus_tag="PLK75_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOY38598.1"
                     /translation="MKRISEISFLFLIILLIATSCVKNKHEANGFIKKGYNDIIARDT
                     LIVAVASNSTDYFIYRGKPMGFQLEILKEFANYLGVELKVVVENSVYKSLAMLITGKC
                     DMVAMSDLSCLPRQAYLSYSQPLANSRLVLVQNGNSKMIKTPEELDGQYIYMVNTGFA
                     SRGLSLFNGKGVYNLHTVLLDSIDSEQLLEGISSGNYTYGVVTENLAMLYNDVLNIKI
                     GAVISDSIPLQWVLPASSQQLLDTLNRWIDSNKEKKLFKTKYNKYYRHFSGEYSIDYS
                     EYMTNKGNISPFDDIIKVQSKKIGWDWRLLASLIYQESKFNPLATSWAGAYGIMQLMP
                     QTASLYNINENSDVDDHITAGVAVIATINNNIPESVCDTLDRIRFTLAAYNLGYSHVN
                     DAILLTRKYGYNSDDWNDVSLFLTHLSDPKFYTDSVVSSGYCPGRLTVKFVDEILERY
                     THYCNILKE"
     gene            complement(7822..8331)
                     /locus_tag="PLK75_04800"
     CDS             complement(7822..8331)
                     /locus_tag="PLK75_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38599.1"
                     /translation="MRIRLLLAIYALLLLILQACFRIETYPPEPQISLKYFTLNDTTD
                     ALGNPGLKGEMCISFIDGDGDIGYNEPADTSLIDSMKTVFIKQYYKVNGIFVESQQQV
                     DYSYRVPFFSTEPNNPSLKGDIIIHDLNSYGPFEGDTIKYTVYIRDRAGNRSNTAETE
                     ELVLINYVN"
     gene            complement(8390..9124)
                     /gene="rlmB"
                     /locus_tag="PLK75_04805"
     CDS             complement(8390..9124)
                     /gene="rlmB"
                     /locus_tag="PLK75_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOY38600.1"
                     /translation="MENYIYGIHPTIEAIKSGKEINKVFIRKGMQSEQLGALLKLLNQ
                     CNIPTQFIPAEAFNKYTRKNHQGVLAIVSPVEYQPYDEIIHRVFASGRDPFVLVLDGI
                     TDVRNFGAIARSAECAGVNAIIITMINNPGITPDAVKTSAGALNTIPVCRVKNLPDTI
                     KQLKNSGLEIVACTSKNDTPYTEHDFKKPLALVMGSEEKGISPVVLKETDYCIGIPVM
                     GSIDSLNVSVATAVVMYEVIRQRKLS"
     gene            complement(9132..10415)
                     /locus_tag="PLK75_04810"
     CDS             complement(9132..10415)
                     /locus_tag="PLK75_04810"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOY38601.1"
                     /translation="MKGNNSVFLIPVFLFVSLIIAVSCYRPPVANPENWQYNFASIYN
                     PGESAINPEYKLYHQSDTTSLLFFRISTDDLIPNTQPDNSEPYKAVVVKYVLRNAENN
                     QIADSASLLFRISATATERDFITYIKLKTPLLTNYNLVVIFSDVLRKVPRRVFIDLNK
                     SDYGNEQNYFFETADSVKAPLFSNVVEGNRKYIFHKKRNPAEKYFISYYKPISSIPQS
                     PFAIQYNASLPSPDTLYAIKPNDTLSFSAKGIYFITEKDAGTEGIALTNFGQGYPEIQ
                     SVKNMILTVRYLTTERKWNEMLKSSDPKTEIDNFWLNLSSDPHRAKELIRVYYNRVIL
                     ANTFFGTYKQGWQTDRGMIYIIFGPPATIYKSSDIEQWIYGDNPELSDISFSFKKTSN
                     LLSDNEYILIRDQRYQTIWAQAIETWLQGRVFSIN"
     gene            complement(10402..14928)
                     /locus_tag="PLK75_04815"
     CDS             complement(10402..14928)
                     /locus_tag="PLK75_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38602.1"
                     /translation="MKCKIVFLLLIAIGLFQTMSVAQTGFDPDHSKFLRQFDSYLQYS
                     GELRKDSKSIYKEFEAFWESDTLTVKEKDDFIATASKMREKGCQEYPEFVTYAINRME
                     FSRLSITEEQYKIYENEIIEFLKPRTIKRNDLAEFLLATYRLINQHIVYYKPKSQWRV
                     STDAFEFAKIGGDFCIKFNNTDLILNYGNDSVKISKTNGVLYPFKELWVGKNGLVTWE
                     RVKLSADSIYANLRNYKVSLKEVKYVADSVTFINSIYFKEPLLGQISDEALNLDDPSK
                     STFPRFRSYQQKFKIKNIVKGVDYEGGFSMRGDEFIGSGVDEKLAHIYIEKNDTISFS
                     AYSKSFHLDPKQIFSEHTTIVLRMSEDSIYHPDVTFKFTIQEGFLELIRLKEGMSQVN
                     FFDSYHQISMDFTWLKWYIDKFLIEITVIQTPGYPNEVLFESLDYYRYERYNEIKMRD
                     ARNPLEYLTEYCYVCNCQEFTANQLAAFVKFSPTQVKQMLLRVAYMGFIIYDPEKEIV
                     KMKPEAWAFIDAYKKTKDSDVLQFYSQTPSQQTNAELSLLNYDLKIMGIPAVNLSDSQ
                     NVKIYPIDKKLILKKNRSFKFNGTIQAGQFYFYGSNFGYNYNRFMLDMPQCDSMKMVA
                     ETDELDSKGNKRPAIVRNKIENFYGEFFIDDPLNKSGRFDYPQYPSFTNKTKSYVYYD
                     AKDIFNGVYKRQKFYFEVDPFTIDSIKGYKRENLKFKGRLVSAGIFPDISETLVLRDD
                     FSLGFRMNTKSAGLPMYDGKAKYNNIIDLSYEGLRGKGKLEYLTSTFQADYLVFFPDS
                     LQGHSETFKMDLQTTPVQFPVVFGADNTLNWQVKRDKFFVYEDSTDFQMYNNQAVHKG
                     FLNITSNGLLGTGTLTIQKAKLSSKLFDFGYQTIDADTANFEIYAFSEFASDFDGKNV
                     NSHVDFASRKGTFKTNGEQTKWEFKYNKYISIMDQMIWHMDKEELEISASSDVLDEIE
                     KSKGKLSPTEWEGLFFDGPEFISVHPGQDSLRFTAPRAKYNYTEHVIYAEGVQLIRVA
                     DATVYLDAKNQVVVIEKDAKMQPINNVEIQANVTTRFHSIYDANVNITGRLKYTGEGY
                     YNYTDDINRKQKLFFNSVSVDYSGQTIASGRITEPDNFMLSPHFAFQGEVRLAASREH
                     LEFIGAVKISPVCDTINTQWLKFASIIDPSDIYIPVDSLPLDINNQRISTGLHFSSGG
                     KAYPAFISRTYTPYDIPIFESHGYLYYDKTDKAYMIASREKIDELKMPGNFMEYYREE
                     CKVYGEGKFDLTKRGELFNLNSIGNFEYDFSDDTIALNLSMLIYAPLTNQAWKRFAAV
                     IQGTQGLEGVDMSSELYEKALVEFLGTNKADEWFSNLSLGNTNKYPDELENMLIITDL
                     QMTLHYGMSSFIHAGQIGIVNAGKNQVSRSVFGFVKAERGKRSDTFEMLIEPDDKTWF
                     YFRYVGGVITILSSDQEFNDILINAKDNDRYFEDKETGGTLSYICGGDTYYKKFKRDM
                     YKKFNLAVESDEGE"
     gene            complement(15068..15895)
                     /gene="prmA"
                     /locus_tag="PLK75_04820"
     CDS             complement(15068..15895)
                     /gene="prmA"
                     /locus_tag="PLK75_04820"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOY38603.1"
                     /translation="MNYIELSIETTSLPEILPEILIANLSQIGFESFVHESAVLLAYI
                     PEKNYNGLKTENLLSPYFVNYFTKLIPEKNWNTEWEQNFKPVVISDKIYIRATFHKKE
                     NYPYELIIEPKMSFGTGHHETTSMMCELMLQTDFRKKNVLDMGCGTGILAVLASKLGA
                     TQVLAIDNDEWAYNNTLENIKLNDVTNVQCSLGDISAIEKELFHVVLANINMNVLKND
                     IATYAKHLKSKGMLLLSGFYDSDINEILAAANASGLVLQDKIVKNNWAACNLIAKSK"
     gene            complement(15897..16658)
                     /gene="tpiA"
                     /locus_tag="PLK75_04825"
     CDS             complement(15897..16658)
                     /gene="tpiA"
                     /locus_tag="PLK75_04825"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOY38604.1"
                     /translation="MRKRIVAGNWKMNTDLVSGKDLAVLINAGIAEGKFPSGVDVILG
                     VPFTHLSSIAAVVDFKKISLAAQNCSAFEQGAYTGEISAAMIKSTKTRYVIIGHSERR
                     QYFQENNEEIASKVRLCLKNELIPIFCCGETKTDREKSVQFDVVGSQLLHGLFHIDDE
                     DMQRTIIAYEPVWAIGTGLTASPAQAQEMHAFIRELLVTRYSKITANMVPLLYGGSCN
                     AQNARQLFAQPDIDGGLIGGASLKHREFFDIINSF"
     gene            complement(16670..18214)
                     /locus_tag="PLK75_04830"
     CDS             complement(16670..18214)
                     /locus_tag="PLK75_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOY38605.1"
                     /translation="MKIIANISRLITGIVFVFSGFVKVVDPLGTAYKFADYFVAMHLD
                     FLIDFALVLSVVMCTAELLIGLALLFNVLPKLASWGLLLFMAVFTPLTLWLAIANPVH
                     DCGCFGDALILSNWATFFKNVVILIFTVLVFLYRKKFRPLFGKPMQWALVTGFTIFGL
                     SVCFYALMHLPPVDFRPYKIGANIQEGMQIPESEKNNVDVYESTFIYEKDGVKKEFTL
                     ENLPDSTWKFIDAKHILIKQGYVPPIHDFTIEPVVLNAMPQEETETPDIYDALYSFEK
                     DGELNDYTIDEIPDNSWIFIEVKSAQSIDPENIELVYQNPDGEDETFTLFNLPGEEYN
                     FVEAYYVSSSGNQESGPVEEGDISDMVLAYPEYTFLFISVDLDEVNIKNHDEINKLAA
                     FCEAKGYHFLCLTASSAEGVEKFIGKYKPIYNFYNTDPITLKTIVRSNPGLVLLKEGT
                     VLNKWSYRDLPTVDELQGNLEAYSISELYAKRTHFLIHNFIFIGLLVMTLLMWLQHSY
                     KSKRRN"
     gene            complement(18292..18981)
                     /gene="tmk"
                     /locus_tag="PLK75_04835"
     CDS             complement(18292..18981)
                     /gene="tmk"
                     /locus_tag="PLK75_04835"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOY38606.1"
                     /translation="MSFIVIEGLDGSGKSTQVKMLREFFESCQIDYRYLHFPIIESPF
                     FGDLIARFLRGDLGSIESVDPYIVALLYAGDRYNAKAQIEDWQNNNLLVLTDRYVYSN
                     IGFQCAKANDPESAATLYRWILDLEYKYFNVPKPDLSIFLDVPMEFVEQKLAEQREDE
                     ARGYLLGKADIHEADISFQKRVRDMYLMAVENDASFLKINCISGDGKMLPPETIFHSI
                     VQLINSKHLLK"
     gene            19090..19950
                     /gene="folP"
                     /locus_tag="PLK75_04840"
     CDS             19090..19950
                     /gene="folP"
                     /locus_tag="PLK75_04840"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOY38607.1"
                     /translation="MSTKLLKIKGEYLQIDTPLVMGIVNISTNSFFSGSRVYNEENLL
                     RKVERMLWDGADIIDIGAVSTQPGAVLLSAEEEWARLAGSLYAIRKTFGNDIVVSVDT
                     VNANVASMAVREYGVDIINDISGGQVDEKMFEIIAGINVPYIVMHMRGTPDTMQQFTS
                     YNNLMGDILYYFSERLNRLHQFGVSDIIIDPGFGFSKNIEQNYELLQHLESFHIFEKP
                     ILVGISRKSMIYKTLNCSPEEALNGSTALHTVALMKGADILRVHDVKEAKETIKLIGV
                     LKSKENPEWI"
     gene            19978..20754
                     /gene="cdaA"
                     /locus_tag="PLK75_04845"
     CDS             19978..20754
                     /gene="cdaA"
                     /locus_tag="PLK75_04845"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOY38608.1"
                     /translation="MIPLFIEIRFLDVIDILLVAFLLYQLYMLIRGTVAIRIFIGIFS
                     IYIFWLFVKAMKMDLLASLLGQVIGVGVIALIIVFQQEVRKFLMMLGNRYFNRKRFNF
                     YKLFTINNTTSRGHLDVIVESCFTMSKEKTGALIVIAKDSDLKSFTETGAMLNADIND
                     RLIRNIFFKNSPLHDGALIVVGNKIAAAGCILPVSNHPGLADNYGLRHRAALGLTEMS
                     DSLVIVVSEQTGNISVSKNGKLYPVTSSERLVKMIEKQFS"
     gene            complement(20755..22731)
                     /locus_tag="PLK75_04850"
     CDS             complement(20755..22731)
                     /locus_tag="PLK75_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOY38609.1"
                     /translation="MKNRKPELLLPAGNTEAFFAAIEGGADAVYLGLKQFNARGRAAN
                     FTSSELSGVIKVARERKIKVYVTLNTLVKNREIKSILEQLKLLKALKPNAIIIQDWGV
                     YFLIKNYFTGIEIHASTQMGIHNSCGANHALQKGFSRAIIARELTRAEIATMVKQTNM
                     PLELFVHGALCYSFSGMCLFSSFIGGNSANRGNCMQVCRREFAVEGSEAFLPFNLRDN
                     QLIQYIKDITELKIAALKVEGRLKQAEYVFKTAKAYRLAIDNPEQYDKALKILESDGG
                     RDKTSWFFGNNVSNSLSQSNQTGNFTGIVCRVTREGFEFESIVHLKKGARIRINTSSF
                     QDPFYFTVTSVSGNLAVVTAPGHTIANGDKVYVTSFDGMRFSEKLPFSTFGQKKAETS
                     GAGQHIAAYKNSKVSGKTELYCRISGTYDLKHIDSLAVSGIFIKITDDILKNKSWVEA
                     CKNIRHKLYFEFPKFISEQRIADYRNIACELKGNGFNNFVINQLSQRLLLPSGSRFIT
                     GENVYVMNDFTALAIKSEGASLFVYPFENDYPNMITGKSRDGIVPLHFYPDLFYSRMP
                     VHHSKDKTLKLPGYKGGFTAKVENGITIIRPEQPVSLFRFKEKLIAKGFFRFLIDFSA
                     SASNKDSFCTIMNAYFRGEKIQGSSEFNFKMELK"
     gene            complement(23254..24906)
                     /locus_tag="PLK75_04855"
     CDS             complement(23254..24906)
                     /locus_tag="PLK75_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY38610.1"
                     /translation="MNLKLNRAIFIVACFILCLQISLNAQQISFSFTANHACEYTQLD
                     SVLIENTTLGCDTTLYWNDTVISFSFTGMSDVMSLQNASFVAECAPNPFDLEANIYLE
                     VIKPDNFIFEIFDIAGRKLLVSTIHLNQGTHIFTLIAGSSFQYIINVGSSNHHQCLHL
                     IQTNHQANKVSLVYMGLVNGYNRNNKKKSGKAWFNYCIGNELRFTGFAQGTAEEITDI
                     PSESMYYTFNIDGEIPETPQQGAIISGLDQISWNWFPVAGASRYTWSTSCEFETSNDI
                     GTDTSLTLEGLYCGTEYTIYIWAYNACGSSEALMLTQETLFSCGCTFADNRDANTYQT
                     VQIGSQCWMAENMAYLPLVHTDAEFQSLGSWIQRAYGVYGYNGSDVELAKAHENYATY
                     GVIYNWWAAMDGYVGSSTNPSGLQGICPKGWHVPSDDEWKQLEGEVDATYPYGDPEWD
                     LEWFRGDDAGSALAGNAPLWTDDNLINHSSFNSSGFNILPGGQRDQWGNSIAMGGHTY
                     LWSATEDSQPSSWVRAIYYDDPSVYRNPVNKQWGFYVRCVRN"
     gene            25102..>26254
                     /locus_tag="PLK75_04860"
     CDS             25102..>26254
                     /locus_tag="PLK75_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38611.1"
                     /translation="MKKNFVIRLILLVSLFSLCIVTGCNEDANFFETIFAKKVKIKGK
                     IDRSKSTNSDGTRASDNDYTLDQAEKVLIFYGRKYDLVNIHNDGTFTGRAPLGSATAV
                     AFLTQDNQFIGNLFTGGINFIPLVSDNGDLTEIDLDELVLDGDRVIPANDPLENQIIL
                     SDAELEFMQEVGAYYKSLSKNIDMNNDGTPDAMQHGVIDITICSSFFAGKFGVNEFEP
                     ELGTFDQSSINYTIFMEGPIEWVSSSDNSIPENATITGPEENPCNDLRNAGNSYSNHT
                     NFKVNFAKGSQDGVFQPFPPGIYTLHIDNKEFTFYYSSLNTFNYWVLAIPTLITNDAG
                     EVTSIRLEYQFPNGTTIEPRNLISTGISVDISGMNYDNIYSYMAESNSSS"
BASE COUNT         7816 a         5408 c         5138 g         7892 t
ORIGIN      
        1 ttcagtatct gacacttaca aaattgccta ttaaaaagtg tcgaagtcag gaaaacggat
       61 caatggttag agggcaattt tcggaggcac tactataaac atattcaaaa tcatttacta
      121 ctaagccggc tcttaccgga ttttgatgga tgtagttttg cttcgtaaga aatgtttcat
      181 gattgttgat tagaataggt tgattatcgt tttgccagaa ctgatggaat ttatttaatg
      241 gattattaaa tgcggcgttt ttaaacgcgt taatcatcca atcccttcta ctttctctag
      301 tatttttatc aatcatttta aatagttctt ttgaggtata tgttttgaaa tcacgaagaa
      361 tattacctaa tggctcaatt ttcgatcgga ctatcaggtg tatatgattc gacataatta
      421 caaaagcata gatttcaagt cctttttcgg tacggcaata atttaggttt tcaacgatga
      481 aatccatata ttcacgtctc gtaaaaacat caatccaatc tattacagta agagtgatga
      541 aatatgcttc gttatcgcaa gcccttaatt ccatgatata aaaatacaaa attgtacctt
      601 ataaagaggt ttataatttc aatattaagg attttcactg cagaaagcat tcatgattgc
      661 agcaagcgtg gacgcttgct gcataacaaa attctttttt gtgtcctctg ttaggcagtc
      721 ggtgttcacg ctatttgcat aattggcaat attcttctta aacccagaga caaaacacat
      781 gattgcagca agcgtggacg cttgctgcat aacaaggatt tgaattagag aagatgtatt
      841 tgaaaaattc tggatttaac tataacatcc caaaggtaaa acactcattc tccctgaaaa
      901 atattaattt cgctgaaaat ttactgatat gtatcgtaca cacaattgcg gagagctccg
      961 catggcaaac accggaacca aggtaacact atgcggctgg gtgcaacgca aacgcgacaa
     1021 aggcggcatg atatggatcg acctgcgcga ccgctatggc attacccaac tcgttttcga
     1081 acagggcaaa acacccactg atattctcga tatggtgaac gaaaccggac gcgaatttgt
     1141 cgtacgcatt cagggagaag tcattgcccg tcaggctgcc aatcccaacc tacccaccgg
     1201 tgatatcgaa attcgtgtgg aacactacga aattctcaac ccatcggcca ccccgccatt
     1261 caccatcgaa gaaaaaaccg acggtggcga cgacctgcgc atgaaatacc gctacctcga
     1321 catccgccgc aattcggtaa aagacaacct gcttctccgg cataagatgg cacaggtaac
     1381 ccgtaaatac ctcgacaaca ttggttttat cgaagtcgaa acgccttacc tcataaaatc
     1441 aacccccgaa ggtgcccgcg actttgtggt tccttcgcgc atgaatccca accagtttta
     1501 cgccctgcca caatcgccgc aaacattcaa gcaactgctc atggtggccg gtatagacaa
     1561 atatttccag attgtgcgct gtttccgtga cgaagatctg cgtgccgacc gtcagcccga
     1621 gttcacccaa atcgactgcg aaatgtcgtt tgtggagcag gaagatatat tgcagacttt
     1681 cgaaggtttg gtaaaatata tgtttcgcga agtgaaaggc atcgacatta cagaaaactt
     1741 ctttcgtatg ccctacagcg aagccatgga aaaatacggt tccgacaaac ccgatctgcg
     1801 tttcggtatg tgcatttccg aaatgagcca tctggttaaa ggcaagggat tcggagtatt
     1861 cgacgatgca cagtatgtgg gtggtatttg tgccaaaggc tgcgctgttt atacccgtaa
     1921 acagttagac gaactaaccg aatgggtgaa acgtcctcaa atcggtatga aaggcctaat
     1981 ctattgccgt gtgaacgaag atggtacatt gcgctcatca gtagataaat tctacagcga
     2041 agacgacctg aaacaatggg ccaatttgct taatgcccaa gccggcgacc tgattctcgt
     2101 gctggccggc gaccacgaaa aaaccctaaa agcgcttggc gagctgcgtc ttgaaatggg
     2161 taaccgtatg gacttgcgca accctcagaa atttgctccg ctatgggtag ttgatttccc
     2221 gttgctcgaa tgggacgacg aaacacaacg cttctatgcc aaacaccacc cgtttacctc
     2281 acccaaacca gaagacattg ccctgctcga aaccgatgcc ggcagtgtgc gtgcaaatgc
     2341 ctacgacatg gtaatcaatg gtgttgaggt tggtggaggc tcaatccgta ttttcaaccg
     2401 cgaactacag cagaaaatgt tttcggtgct gggctttacc gatgaggaag ccgagcatca
     2461 gtttggcttc ctgctcaacg cattccgcta tggtgctcca ccccacggtg gtatcgcctt
     2521 tggtttcgac aggttgtgtt cactgtttgg tggttccgat tccatccgcg actatattgc
     2581 cttcccgaaa aacaattcgg gacgcgatgt aatgatcgac agtccggctg ccataaaaca
     2641 ggagcaacta gatgagttgt ttcttgattt gaaaaaacct ttataattcc agaacaaaaa
     2701 aatatcttac attatttctg ggtttattat tatttcgatg caaccttttt attaatatca
     2761 aagttactat tatagaaact taacataact ttgttttaat accataattc gctatgattg
     2821 cctcaagaaa cacaattgtt tttatgctaa ttattgttgt cggaataatt tatactggat
     2881 gcaaaaagat tcgaaaccca gtaattacca cacttaatcc cgtatcggta acaattaatt
     2941 cagcagcaat tgaaactact gtttcaaccg atggtgggcc tgatatttcg gaacgcggtg
     3001 tatgctggag tattcaccca actccaacgg taatcagcaa taaaaaagcc tgtgggtcgg
     3061 ggagtggtaa tttcagttgt accgttacta acctggaatc gaatacaacc tattacctga
     3121 agtcgtatgc catcaacgaa aatggagtat attacggaaa cgaggtgcag tttactacgt
     3181 atgatgcgtc agatctggta atggttacca tgaatatttc tttcattaca agccattatg
     3241 caaatgcgta tgtaaatatt aattacacaa atgagccaag ccccacacaa ttcggtataa
     3301 aatggagcaa ttcaccagca atgaccagtt gcgacagcat cattcgcaat tttcaaattc
     3361 ctaataattc acattttaaa atagaaggat tgcaacccgg gacccaatat tacatgcagg
     3421 cgtttgctat caatgatgca ggaacttcaa ccagcacagc aattgatgtt actacggtat
     3481 cggcaaacca agcaccggag atacaaactc atgaagcata cgacattatg caatacgggg
     3541 catcttttcc tgccgaaatc atcagtcatg ggtcggatac aatcaccgaa tatggcgtat
     3601 acgtatcaac cagcaatcag tttgatgatg ccacaggcaa tacatttaag ctgataatgc
     3661 aaaacagtaa cacatatttt cttacaaata ccctgctccc tgaaacccat tattattaca
     3721 gggcttatgc cattaatgcc gtagggactg cctatggtgc aacaaaaaac ctgacaacac
     3781 ctgatgatga tcagatacat ttttccgatg gcgtacaata tggaactgta acggatattg
     3841 atggaaattc ttacagaaca atccaaattg gcaatcaaaa atggtttgcc gaaaacctgc
     3901 gtaccagccg ttaccggaat ggtgaattaa ttccggagat ttccgaattt tccgactgga
     3961 caaacagcac tcagggagca tggtgccatt atgataatga aagcaggaat gaaataccct
     4021 acgggaaatt gtacaactgg tatgcagtta gcagcaacga tgggttatgc cctgccggat
     4081 ggcatgtcgc ttcgcacaca gaatggttag agctgcccga aaatatggaa acaactgcgg
     4141 gttttgccgg tcacttcatg cgtgaacaag gcatacactg gattcaaaac actgcatgtt
     4201 ccagtaattc cagcggattt accgcccttc cgggagggtg ccgaacaagt aaggacaatt
     4261 ttgacgggca gcgggattat gcgtatttct ggagctcaac ccagtacaac gcgtcaaacg
     4321 caatattctg gcaattgtat ggcaattccg atcaattcag tgccgacaat atcggcaaaa
     4381 gttcgggttt gtcggtgcga tgtataaaag actgaggctg ttgttaaaga gaatcccact
     4441 ttctttgcag ggctcacgga acaatatacc gaattgttag tctcctgatt ttccagccat
     4501 ttcttttatt atttttgcct catgaaaaaa tgcctgacga taatagcatt ttgttttata
     4561 gtttacgctg tcagggcgca gcatgagggt attgagaggc attttgatga atatattttc
     4621 ggggacttta ccacgcgtgg aaattattat tcatccgaca cacttaccga tagttttcat
     4681 ctattccgca ataccgataa ttttatatac agcgcaggca gtattggttc agcacatata
     4741 cccatgtttt attacaaaca gtaccaagat gctgattttg tattttttac accatatacc
     4801 ccatttatca gtacattaca aggttcaacc tatttcgata cgcgtacacc atttacacgt
     4861 attgtattcg ttggtggtga aaagcaactc gataacgtca ggctggtaca cacccaaaat
     4921 atcaggccat ggctcaactt cggggctgag ttcaacagcc tcaacaacga ggggcattat
     4981 gccatgcaac agacaaaaat tcaatcgctg aatgcattcg tttcaataac aaaacaccga
     5041 tataataatc acctgaactt catttacaat aaaatatcgc atgagaataa tggtggaatt
     5101 gaatcagatg aaatatttga ggcagggtcg cagcgttccg aaaatttgag cgtaaacctt
     5161 ttcagcagta caaccaagct ttcgcagatt ggcttttcgt attaccatga acttcttatc
     5221 gggaaaaaac ttatcgacac aatttatcag aacaacgata ccattcagaa aatttcgtac
     5281 aagtcgcggc tgatattcag tcagagtctc tcgtacaatc gttattaccg tgtttatcat
     5341 gatgtgcccg gagatttcta cgaaaacatt tacattgatt caaccgagac ttatgattcg
     5401 gtttcgctgc ggaagttttc ccattcgttc gatgcaaaat atctgataca aggagaagcc
     5461 aacagcatac cccattccat tgctatggct ggttttatta ccgacttgtg ctggtatgga
     5521 aacaacgatt ttttaaccga aaacagcatt tatggccggt ggttttccga cacccgccga
     5581 ttgcaatggg atgtaacggg gaggttttac tttacgggct actatgcagg cgaccttagc
     5641 tcgattgtac aacttctata ttatcccgat aaagacaaaa atcatatgct caagttttca
     5701 cctggatatt cacttacacg tccggactat ttctattcac attaccacgc caaccatttt
     5761 atgtggggga ccaacctgcc gaaaagcagc cgcacaaacc taagtcttac atacgaatat
     5821 gtaaaatggc aattacattc gtcgctcagc tatcaatatc tcgatggatt tttatatttc
     5881 gatacagacg caaaccccaa agtaagttct gcaaattgtc aggttgtatc agcagaaatc
     5941 gggaaaaagc tgaatgtggg aaaattccac tggtacaacc agttgcagct gcaatatctg
     6001 agtaatgatt ccattttatc gcttccgttg ttgggtgctt atagttcgtt gtattttcag
     6061 tcacctttgt ttaaaaaagt acttattctt caaattgggg ttgatatgac ctatttcagt
     6121 agtgctttcg gttatgcgta tatgcctgcc accggaatat tttaccgcaa caccgagaaa
     6181 aaatatggca attattttgt atttgatgcg tatgccagca ttaaggtaaa gcgcttcagg
     6241 gcatttgtaa aagcatcgca tttaaacgat ttctttatgc cttccaatta ttatggttta
     6301 cttcattatc cggctgatcc tttttcggtg aatttcggca tcagctggga attttataat
     6361 taaacactcg caattattga agcggatttc agaaatatca ttcctgtttc tcatcatact
     6421 cttaatcgcc acaagttgtg ttaaaaacaa acacgaagca aacgggttta ttaaaaaagg
     6481 ctataacgat ataattgcaa gagatactct tatagtggcc gttgccagta actcaaccga
     6541 ttacttcatt tatagaggca aacccatggg ttttcaactg gaaatactca aagaatttgc
     6601 gaactatctc ggcgttgagt taaaagtagt tgtggagaat agtgtgtaca aatcgttggc
     6661 aatgctcatt accggcaaat gcgatatggt tgcaatgagc gacttaagct gtttaccccg
     6721 gcaggcatat ctcagttatt cgcaacctct ggccaattcg agattggttt tagtgcaaaa
     6781 tggaaattca aaaatgataa aaacacccga agaacttgac gggcaatata tatacatggt
     6841 gaatacaggc tttgcaagca gaggtctaag tttatttaat ggtaaaggtg tatataattt
     6901 acataccgtg ttgctcgata gtattgactc agagcaattg cttgagggga tctcatcggg
     6961 taattatacc tatggggtcg tcacagaaaa cctcgctatg ctttataatg atgtgctgaa
     7021 tatcaaaatc ggtgcggtta tttctgacag cattccttta caatgggttt tacctgcaag
     7081 ctctcagcag cttctcgata cactcaaccg atggatcgac agtaataaag agaaaaaact
     7141 gttcaaaaca aaatacaaca agtactaccg gcacttctcc ggggagtatt ccatcgatta
     7201 ttccgagtat atgactaaca aggggaatat tagtccgttc gatgatataa ttaaagtaca
     7261 gagtaaaaaa atcggctggg actggagatt gcttgcatcg ctcatttatc aggaatcgaa
     7321 attcaatccc ctggctacat catgggctgg tgcatacggg atcatgcaac tgatgcccca
     7381 gacagcatcg ctgtataata tcaacgagaa ctccgacgtg gacgatcata ttacagcggg
     7441 agttgctgtt attgcaacaa tcaacaacaa tatacctgaa agtgtatgcg acaccctcga
     7501 cagaattcgt ttcacacttg ctgcgtacaa tcttgggtat agtcatgtga acgatgccat
     7561 attgcttacc cggaaatacg gctacaacag tgatgattgg aacgatgtaa gtctattcct
     7621 tacccatctg tcggatccaa agttttatac tgacagtgtt gtgagttccg gctactgtcc
     7681 gggcaggctt accgtaaaat tcgttgatga aattctggaa aggtacacgc attactgcaa
     7741 tattttaaaa gagtaatgag ttttaatcat ttttttgatt tcaggtgatt tttgttaaaa
     7801 gtgtattgag tacacaaagt tttaattgac atagttgatc aacaccaact cttcggtctc
     7861 agcagtattg ctgcggttgc cggctctatc tctgatatac actgtatatt taatggtatc
     7921 gccttcgaaa ggtccgtaac tgttcaggtc gtgaatgata atatcgcctt ttaagctggg
     7981 gttatttggt tctgtcgaaa agaacggaac acgataacta taatcaacct gttgctggct
     8041 ttcaacaaaa atgccattta ctttataata ttgcttaata aaaaccgttt tcatcgaatc
     8101 gatcaaactt gtgtcggcag gttcattata accgatatca ccatcaccat caatgaaact
     8161 gatacacatt tctcccttca gtccgggatt ccccaatgca tcggtcgtat cgttcaatgt
     8221 aaaatattta aggcttatct gaggctcagg cggataggtt tcgatacgga aacaggcctg
     8281 aagtatcaga agaagtaatg cataaatcgc caataaaagt cgaattctca tactgcaaaa
     8341 gtaagcatag ttttattata actacgatac gtccgtttta ttttggccat tacgatagtt
     8401 tgcgctgtcg gattacctca tacatcacaa cagcggttgc caccgataca ttcagggagt
     8461 cgatactgcc catgaccgga attcctatgc aataatctgt ttctttcagt actaccggag
     8521 aaattccctt ttcttcggaa cccataacca aagccaatgg ctttttaaaa tcatgttccg
     8581 tataaggagt atcatttttc gaagtacatg ccactatttc cagtccgctg tttttaagtt
     8641 gttttatggt atccggaagg ttttttaccc ggcatacagg gattgtattc aaagcaccgg
     8701 cagaagtttt taccgcatca ggagtaatcc cggggttgtt gatcatggta ataataatgg
     8761 cattaacccc ggcacattcg gctgagcggg caatcgcacc aaaattgcgg acatcggtaa
     8821 taccatcgag cacgagaaca aacggatcgc gacctgaagc aaaaacgcga tgtattattt
     8881 catcataagg ctggtattct acaggcgata ctatagccag caccccctga tggtttttac
     8941 gtgtgtattt attgaaggct tctgcaggga taaactgagt tggtatgttg cactgattca
     9001 atagcttcag aagtgctccc agctgttcgc tctgcatacc cttgcgtata aaaaccttgt
     9061 ttatttcttt acccgatttt atggcttcga tagtcgggtg aataccgtaa atgtaatttt
     9121 ccataataat tttaatttat cgaaaacacc ctaccttgca accatgtctc gatagcctga
     9181 gcccaaatgg tttgataccg ctggtcgcga attagaatat attcgttgtc tgatagtaaa
     9241 ttcgaagttt ttttaaatga aaaactgatg tcggaaagct ccggattatc tccataaatc
     9301 cattgttcaa tatcggagct tttataaatg gtagctggcg gaccgaaaat tatataaatc
     9361 atacccctat cggtttgcca accttgtttg taagtaccaa aaaatgtgtt ggcgagaatc
     9421 accctgttgt aataaacacg tattagctct ttcgctctgt gaggatcaga actgagattc
     9481 aaccaaaaat tatcaatttc cgttttagga tcagacgatt tgagcatttc attccatttt
     9541 cgctctgtgg tcaagtaacg aacggtaagg atcatatttt tcacagattg tatttccgga
     9601 tatccttgtc caaaatttgt gagagcaata ccttctgttc ctgcatcctt ttcagtaata
     9661 aaatatattc ctttcgcact aaaacttagg gtatcgtttg gtttaattgc atacagtgta
     9721 tcaggagagg gcaaggaagc attatattgt atagcaaacg gcgactgggg gatgctgctg
     9781 attggcttgt aataagatat aaagtacttt tcagcgggat tccttttctt atgaaagatg
     9841 tattttctat taccttcaac aacattacta aaaagcggag cttttactga atcggctgtt
     9901 tcgaagaaat aattctgttc gtttccgtaa tccgatttat taagatcaat aaaaactctt
     9961 cggggaactt ttcgaagaac atccgagaaa atgaccacca aattgtagtt tgtaagaagc
    10021 ggcgttttca gttttatgta agtaataaaa tctctttcgg ttgcagtagc acttattctg
    10081 aaaagtaagg atgcactgtc ggcaatctga ttgttttcgg catttctcag cacatatttc
    10141 acaacaacgg ccttgtaggg ttctgaatta tcaggttgtg tattgggaat aaggtcatca
    10201 gtgctgattc tgaaaaacaa gagggatgtc gtatcgctct gatgatacag cttatattcg
    10261 gggttaattg ccgattcgcc cgggttataa attgaagcaa aattatactg ccagttttca
    10321 ggatttgcaa ccggtgggcg atagcatgaa acagcaataa tcaggcttac aaataaaaaa
    10381 actggtatta aaaaaacact gttattcccc ttcatcggat tctaccgcta aattgaactt
    10441 tttatacatg tcgcgtttaa actttttgta atatgtgtcc ccgccacaaa tatatgataa
    10501 ggttccaccg gtttccttat cctcgaaata tctgtcatta tctttcgcat taataagtat
    10561 atcgttgaat tcctgatcag aggacagtat ggtaattacg ccacccacat atctgaaata
    10621 aaaccatgtt ttatcgtcgg gttcgataag catttcaaat gtatcgctgc gtttgcctct
    10681 ttcggcttta acaaatccga agactgagcg acttacctga tttttacctg catttacaat
    10741 cccaatctgg ccggcatgta taaatgagct cataccatag tgcagtgtca tttgcaaatc
    10801 ggtgataatg agcatatttt ccagttcatc agggtatttg ttggtatttc cgaggcttaa
    10861 attggaaaac cattcgtcgg ccttattcgt acccaaaaat tcgaccagtg ctttctcata
    10921 taattcacta ctcatatcaa caccttcgag accctgagta ccctgaataa ctgccgcaaa
    10981 tcgcttccat gcctgattgg taagcggagc atagataagc atactcaggt tcagcgcaat
    11041 agtatcgtcg ctgaaatcat attcaaaatt tccaatggag ttaagattga aaagctctcc
    11101 tcttttagtc aggtcaaatt ttccttcacc atacaccttg cattcttcgc gataatattc
    11161 cataaaatta ccgggcattt tcaattcgtc gattttctca cgtgatgcaa tcatgtaggc
    11221 cttatcggtt ttatcgtaat acagataccc atggctttcg aatattggaa tatcgtaagg
    11281 ggtataggtt cttgaaatga aagcagggta tgctttaccg ccagaagaaa agtgtaatcc
    11341 tgttgaaatc ctttgattat taatatcaag tggtagtgag tcgactggaa tataaatatc
    11401 agacgggtcg ataatcgaag caaatttgag ccactgtgtg ttgattgtgt cgcagacagg
    11461 acttattttt acagcaccga taaattcgag gtgttcccgg ctggcggcaa ggcgtacttc
    11521 accctgaaac gcgaaatgag ggctcaacat aaaattatcc ggttcagtaa ttctcccgga
    11581 agcaatggtt tgcccactat aatcaacaga tactgaatta aaaaacaatt tttgttttcg
    11641 gtttatatca tccgtataat tataatagcc ttctcccgta tatttcaacc tacccgtgat
    11701 atttacattg gcatcgtaga tactgtgaaa acgcgtagtg acatttgcct gaatctcgac
    11761 attattaata ggttgcatct tagcatcttt ctcgataact acaacttgat ttttagcgtc
    11821 caggtacacg gtagcatcgg caactcttat tagttgaaca ccctcggcat aaatgacatg
    11881 ctctgtgtaa ttatatttgg ctcgtggtgc tgtaaacctt aagctgtcct gcccggggtg
    11941 cactgatata aattcgggac cgtcaaaaaa taggccttcc cattctgtgg gacttaactt
    12001 ccctttgctc ttttcaattt catctaaaac atcactggat gcggaaattt cgagttcttc
    12061 tttatccatg tgccagatca tttgatccat aatactgatg tacttattgt atttaaactc
    12121 ccatttggtt tgctctccgt ttgttttaaa agttcctttt ctgcttgcaa aatcaacatg
    12181 agagttcaca ttttttccat cgaaatcaga tgcaaattcg ctaaaagcat atatttcgaa
    12241 attagcagta tcggcatcaa tagtctgata tccaaaatcg aacaattttg aactgagctt
    12301 tgctttttga attgtcaggg tccctgtccc aagcagtcca tttgatgtta tattcagaaa
    12361 gcctttatgc accgcctgat tattgtacat ctgaaagtcg gtcgagtcct cataaacaaa
    12421 aaacttgtcg cgcttaacct gccagttgag tgtattgtcg gctccaaata ctaccgggaa
    12481 ttgcaccggg gttgtttgca aatccatttt aaaagtctcg gaatgtcctt gcagtgaatc
    12541 cggaaagaaa accagataat cggcctgaaa agtacttgtc agatattcaa gcttaccctt
    12601 tccgcgcaag ccttcatagc tcaggtcaat aatattgtta tattttgctt taccatcata
    12661 cattggcaat cctgcagatt tcgtattcat tctgaaacca agagagaagt cgtctcttaa
    12721 gacaagtgtt tcagaaatat cagggaatat acctgcagaa accagtcttc ctttgaactt
    12781 aaggttctct cttttatagc ctttaatcga atcgattgta aaagggtcaa cttcgaaata
    12841 gaacttctgc cttttataca ccccgttaaa aatatccttt gcatcgtaat aaacatacga
    12901 ttttgtttta ttagtaaagc ttgggtattg tgggtaatcg aagcggccag atttattaag
    12961 aggatcatca ataaaaaact ccccataaaa attttcgatt ttattcctta caattgccgg
    13021 gcgtttattt cccttagagt ccaactcatc ggtttcagca accattttca tactgtcgca
    13081 ctggggcata tcgagcatga accgattgta gttataaccg aaattgctgc cataaaaata
    13141 aaattgccct gcctgaatag taccattaaa tttaaaggac ctgttctttt taagaataag
    13201 ttttttatcg atcgggtaaa ttttaacatt ctgtgagtcg ctcagattca cggcgggaat
    13261 acccattatt ttcaaatcgt aattgagcag actaagctct gcgttggttt gctgcgaggg
    13321 tgtttgagaa tagaattgca gcacgtcgga atctttagtt ttcttatagg catcgataaa
    13381 agcccatgct tcgggtttca ttttaacaat ttccttttcc ggatcataaa taatgaatcc
    13441 catatatgct acacgcaaca acatttgctt aacctgggta gggctgaatt ttacaaacgc
    13501 agccaactga ttggcagtaa actcctgaca attacacaca taacagtatt cagtaagata
    13561 ttcaagaggg tttcttgcat cgcgcatttt aatttcgtta tacctttcgt acctgtagta
    13621 atcaagcgat tcaaacagta cctcgttagg gtatccgggg gtttgaataa cggttatttc
    13681 aatcaggaat ttatcgatgt accatttcag ccaggtgaaa tccattgaaa tctgatgata
    13741 actatcgaag aaatttacct gcgacatacc ttccttcagt cggatcagtt cgaggaagcc
    13801 ttcctgaata gtaaacttaa acgtaacatc gggatggtaa atcgaatcct cgctcatccg
    13861 taatacaata gttgtatgct cgctgaatat ctgtttcggg tcgaggtgga agcttttgga
    13921 atacgctgaa aagctgatag tatcgttctt ttcaatatag atatgagcca gtttttcatc
    13981 aaccccgcta cctataaatt catctccgcg catactaaaa ccgccttcat agtcaacgcc
    14041 tttaacaatg tttttaatct tgaatttctg ctgatatgaa cgaaaccgcg ggaaggtcga
    14101 tttggaaggg tcatccaaat tcagtgcctc atcgcttatt tgcccgagta agggttcttt
    14161 gaagtaaatg ctatttataa atgttaccga atcggcaaca tatttaactt ccttaagcga
    14221 aaccttatag tttctgagat ttgcatatat cgaatccgca gaaagtttca ccctttccca
    14281 agtaacaagg ccgtttttac caacccaaag ctctttaaat ggatatagta caccgttggt
    14341 tttggaaatt ttcacgctat cgttgccata attaagaatc aggtcggtat tgttgaattt
    14401 tatgcaaaaa tcccccccta ttttagcaaa ttcgaacgca tcggtgctta cacgccactg
    14461 agattttggt ttataataca cgatatgttg attaataagt cggtatgttg ccaacaggaa
    14521 ttcagccaaa tcgtttcgtt taatggttcg gggtttcaaa aactcgatta tttcattctc
    14581 ataaatctta tattgttctt ctgtaattga caacctcgaa aactccattc tgttgatagc
    14641 ataggtcaca aattcgggat attcctgaca gcctttttcc cgcatttttg aagctgtcgc
    14701 aataaaatcg tctttttctt tcactgttag agtatcagat tcccaaaaag cttcgaactc
    14761 tttgtatata ctttttgaat ccttccgcaa ttcgccggaa tactgcaaat acgaatcgaa
    14821 ctgtcggaga aatttagagt gatcggggtc gaagcctgtc tgtgcaacag acatagtttg
    14881 aaataaccca atcgcaatta gtaaaaggaa aacgatttta catttcatgt atatgcaatt
    14941 taattaccac aatcaaatat caagcctctt cgctttcaac atcaatgtac ccaataagta
    15001 agtcttttac cgcaccacgg acttaaccat tcacttaaaa atgcaaaaaa taaacaagcg
    15061 ttgctgtcta tttcgatttc gcaattagat tacaggcagc ccaattgttt ttgactattt
    15121 tatcttgtaa aacgaggccg gatgcattgg ctgcagccag tatttcgttg atatcagaat
    15181 cgtaaaatcc acttaataag agcatgcctt ttgacttcag gtgcttagca tatgttgcga
    15241 tgtcattctt cagcacattc atatttatat tagcaagaac aacgtgaaat aactcctttt
    15301 ctatggcaga aatatcaccc aacgagcatt ggacgttggt aacatcattg agtttaatat
    15361 tctcgagggt attattatac gcccattcat cgttgtcgat tgcaagaacc tgagttgctc
    15421 ccagtttgga agctaaaacc gccaaaatac cagtgccaca tcccatgtcc aatacatttt
    15481 tcttacgaaa gtcggtttgc aacatcagtt cgcacatcat cgaagttgtt tcatgatgtc
    15541 cggttccaaa cgacattttc ggttcaataa tcaattcgta agggtagttt tcttttttat
    15601 gaaaagttgc cctgatatag attttatctg atataacaac cggtttgaag ttttgctccc
    15661 attcagtatt ccagtttttc tccgggataa gttttgtaaa ataatttaca aaatatggcg
    15721 acagaagatt ttcggttttg aggccattgt aatttttttc ggggatatat gccagcaaaa
    15781 ctgcactttc atgtacaaaa ctttcaaagc caatttggct caggtttgct atcagaattt
    15841 caggcagaat ttcaggcagg ctggtcgttt caatcgaaag ttcaatatag ttcatgctag
    15901 aaagaattta ttatatcgaa aaattcacgg tgcttcagcg atgctcctcc aataagtcct
    15961 ccgtcgatat cgggttgtgc aaataattgt cgggcattct gtgcattaca gctgccccca
    16021 tataacaagg gtaccatatt ggcagtgatt ttactgtaac gggttaccag caattccctg
    16081 ataaatgcat gcatttcctg tgcctgggca gggctggcag tcaagcctgt tccaatggcc
    16141 caaacgggtt cgtatgcaat tatcgtacgt tgcatatctt cgtcgtcgat atgaaacaat
    16201 ccgtggagca actgacttcc aaccacgtca aactgaactg acttctctct gtcggttttg
    16261 gtttcaccgc aacaaaatat cgggatcaat tcgtttttaa ggcataagcg cacttttgaa
    16321 gcaatttctt cgttgttttc ctgaaaatat tgtctgcgtt cactatgtcc gattattaca
    16381 tatcgggttt tcgtgctttt aatcattgct gctgaaattt ctccggtata agcaccttgt
    16441 tcaaaggcag aacagttttg cgctgccagc gatattttct taaagtctac cacagcagca
    16501 atagagctga gatgcgtaaa aggtacaccc aaaataacat caacgcctga agggaatttt
    16561 ccttcggcaa tacctgcatt aatcaatacc gcaagatctt tacccgatac caaatcggtg
    16621 ttcattttcc agttaccggc aacaattcgt tttctcatct ttcttaatat taatttctac
    16681 gtttcgattt ataactatgt tgcagccaca tcagcagcgt cataaccaat agtccgataa
    16741 atataaaatt atgaatcagg aaatgggttc tctttgcata gagctcactt attgaatatg
    16801 cttcgagatt gccctgcaat tcatcgactg ttggaagatc gcgatacgac cacttgttca
    16861 gaacggttcc ttccttcagt aatacgagtc ccggattgga acgaacaatg gttttcaagg
    16921 taattgggtc ggtattataa aaattataaa tgggtttata tttcccgatg aatttctcaa
    16981 caccctctgc agatgaagct gttaggcata agaaatgata gccttttgcc tcgcaaaaag
    17041 cagcaagttt attgatttca tcatggtttt taatgttaac ctcgtcaagg tcaactgaaa
    17101 taaataggaa agtatattcg gggtatgcaa gtaccatatc cgaaatatcg ccttcttcca
    17161 ccgggccgga ttcctgattg ccagacgatg aaacatagta agcttctaca aaattgtatt
    17221 cttcgccggg tagattgaac agtgtgaaag tttcatcctc tccatcgggg ttttggtaaa
    17281 cgagttcaat gttttccggg tcgatagatt gcgcagattt cacttcaata aaaatccagg
    17341 agttatcagg aatctcatcg atagtatagt cgttcagctc gccatctttt tcgaaagaat
    17401 acagtgcatc gtaaatatca ggggtttcag tttcctcctg aggcattgcg ttaagaacca
    17461 caggttcaat ggtaaaatcg tgtattgggg gtacatatcc ctgttttatt aatatgtgtt
    17521 ttgcatcaat aaatttccag gtagaatcgg gcaaattttc gagggtaaac tcctttttaa
    17581 caccatcctt ttcgtagata aacgtactct catacacatc gacgttgttt ttttcgcttt
    17641 caggaatctg cataccttcc tgaatattgg ccccgatttt atagggtctg aaatcaaccg
    17701 ggggaaggtg catcaacgca taaaagcata ccgataagcc aaaaatggta aatccagtaa
    17761 ctaaagccca ctgcatgggt ttcccaaata aaggtctgaa ttttttccga tataaaaata
    17821 ccaagactgt aaatatcaat atcacaacat ttttaaagaa ggtagcccag tttgataaaa
    17881 ttagagcatc accaaaacat ccgcagtcgt gaaccggatt tgcaattgca agccataaag
    17941 tcaaaggtgt aaaaacagcc ataaaaagca ggagtcccca cgaagcaagc tttgggagca
    18001 cattaaacaa taatgccaaa ccgatgagca attcggcagt acacataacc accgataaaa
    18061 ccaatgcaaa atcaatgaga aaatcaaggt gcattgcgac aaaataatca gcgaatttat
    18121 aggcagttcc caacgggtcg accaccttaa caaaaccgga gaacacaaag acaattccgg
    18181 taatcaagcg agaaatattt gcaataatct tcataatcaa taaattttca caaatcaaaa
    18241 atacaatatt gaacgcttat aaaaaaacgt ttttcattat aaaacattgt ttcacttcag
    18301 cagatgcttc gaatttatca attgaacaat gctatggaaa attgtttcag gcggaagcat
    18361 tttcccatcg cctgaaatgc agtttatttt taaaaaagag gcatcgtttt ctacagccat
    18421 taaatacatg tctcttactc ttttctgaaa actgatatcg gcttcatgaa tatcggcttt
    18481 acccaacaaa taccctctgg cctcatcttc tctttgttct gccagttttt gttcaacaaa
    18541 ctccattgga acatctagga aaatgctcaa atcgggtttg ggtacattga agtacttata
    18601 ttccagatct aaaatccacc ggtataatgt agcggcactt tccggatcat ttgccttggc
    18661 acattgaaat ccaatattcg aatatacata gcgatcggta agcacgagca aattattgtt
    18721 ttgccaatct tcaatttggg cttttgcatt gtaccggtcg ccggcataca gtaaagccac
    18781 aatgtaggga tcaacacttt cgatactacc caggtcgcca cgtaaaaaac gggcaataag
    18841 atcaccaaaa aacggactct caattatcgg gaaatgcaga taccggtaat caatttggca
    18901 ggactcaaaa aattcacgca acattttcac ctgcgtcgat tttcctgaac catcaagccc
    18961 ttcaataact ataaaactca taaaattttg taatttcgaa agcttaaaaa taaacaatat
    19021 tagcaactaa ctaaacaaag atttatttat aatttaacat tactgataat attagtatta
    19081 ttgccgctta tgagtaccaa attattaaaa ataaaaggcg aatatttaca aattgacact
    19141 cccttagtaa tgggtattgt aaatatttct acgaattctt tcttttcggg tagtcgggtt
    19201 tataatgaag aaaacctcct acggaaggtt gaaagaatgc tttgggatgg agccgatata
    19261 attgatatcg gagctgtttc aactcaaccc ggggctgttt tgttgagcgc agaggaggaa
    19321 tgggcccgcc tggccggttc gttatatgca attcgaaaaa cttttggaaa cgacattgtt
    19381 gtatcggttg ataccgtaaa tgctaatgta gcttctatgg ctgtaagaga gtatggagtc
    19441 gatatcatta acgatatcag cggtggacag gtcgatgaaa aaatgtttga aattattgca
    19501 ggtattaacg tgccgtatat agttatgcac atgcggggaa cacccgatac catgcaacag
    19561 ttcaccagtt acaacaatct catgggagat attctgtact acttttcgga gcgattaaac
    19621 cgtttacatc aatttggagt ttccgacatt attattgatc cgggttttgg attctcaaag
    19681 aatattgagc aaaattatga attattgcag catcttgaaa gtttccatat ttttgaaaaa
    19741 ccgatacttg ttggaatcag ccgcaaatca atgatttata aaacactgaa ctgctcaccc
    19801 gaagaagccc tgaatggcag tacagcgttg catacagttg cattgatgaa aggtgccgat
    19861 attcttcgcg ttcacgacgt taaagaagcg aaagaaacta ttaagcttat cggagtgtta
    19921 aaatcaaaag aaaaccccga atggatttaa acctaaaaat atgtactttt gcatgatatg
    19981 atacctcttt ttatagaaat acgttttctt gatgttatcg acattcttct ggtagccttt
    20041 ctgctctatc agttgtatat gctcatcagg ggaactgttg ctatcaggat attcattggt
    20101 attttttcga tttacatctt ttggcttttt gtcaaagcca tgaaaatgga tttgcttgct
    20161 tcgttgctgg gtcaggtaat tggagttgga gttattgcac tgattattgt ttttcagcaa
    20221 gaagtgcgaa aattcctgat gatgctgggc aaccggtatt tcaaccggaa gcgtttcaac
    20281 ttttacaaat tgttcacgat aaacaacaca acatccaggg ggcacctcga cgttattgtg
    20341 gaatcctgtt tcacaatgag caaagagaag accggagccc tgattgttat tgccaaggac
    20401 agcgacctga aaagcttcac cgaaacaggt gccatgctga atgccgatat caacgaccgg
    20461 cttatacgga atatcttctt taaaaacagt cctttgcatg atggggcact tatcgttgta
    20521 gggaataaaa ttgctgctgc cggttgtatt ctgcctgtat cgaatcaccc ggggctggct
    20581 gataattatg ggttgcggca tcgtgcagct ctgggcctga ctgagatgtc ggattcactt
    20641 gtaattgttg ttagcgaaca aacaggaaat atttctgtgt caaaaaacgg gaaactttat
    20701 cctgtgacca gtagcgaacg gcttgttaaa atgattgaaa aacaattctc ctgattattt
    20761 taattccatt ttaaaattaa actccgaaga gccctgtatt ttttctccac gaaaatacgc
    20821 attcataatt gtacaaaaag agtccttgtt tgatgctgat gccgagaaat cgatcagaaa
    20881 tctgaaaaaa cccttagcaa taagcttttc cttaaacctg aacagtgaaa caggttgctc
    20941 cggacgtata atcgtaattc cattctcgac ttttgctgta aaccctcctt tatatcccgg
    21001 caatttcagt gttttgtctt tactgtgatg aaccggcatc cgtgaataaa acaaatcagg
    21061 ataaaaatgc agaggcacta tcccatccct gctttttccg gttatcatgt tggggtaatc
    21121 gttttcgaat gggtaaacga ataaggatgc accttcgctc tttatggcaa gagcggtaaa
    21181 atcgttcatc acatacacgt tttcaccggt aataaacctt gatcctgacg gaagcaataa
    21241 gcgttgcgac aattgattta taacaaaatt gttaaaacca tttcctttca gttcgcaggc
    21301 aatattcctg tagtcggcta tacgctgttc gcttatgaat ttcgggaatt cgaaatataa
    21361 cttatggcga atgtttttgc aggcttctac ccatgatttg tttttaagga tatcatcagt
    21421 aatttttata aatattccag aaacagccag ggaatcaata tgtttcagat cataagtacc
    21481 cgaaatccgg cagtataatt ccgttttacc cgaaaccttg ctgtttttat aagcagctat
    21541 atgttggcct gcaccactgg tttcagcctt cttctgacca aaagtagaaa aaggtagttt
    21601 ttcactgaaa cgcatcccat cgaaagatgt tacataaact ttgtcgccat tagcaatggt
    21661 atgacccggc gctgtaacaa cagccagatt tccggaaact gatgtaactg tgaaataaaa
    21721 tggatcctga aacgaagaag tattaattct tatccttgca ccttttttca gatgcacgat
    21781 ggactcgaat tcaaagcctt cccgggtaac ccggcacaca attcctgtaa aatttccggt
    21841 ttgattgctc tgactaagcg agttgctaac attattccca aagaaccatg aagttttatc
    21901 acgaccgccg tcagactcaa gtatttttaa tgctttgtcg tattgctccg gattatcgat
    21961 agccaagcga taggcttttg ctgttttaaa gacatattct gcctgtttta gccggccttc
    22021 aacttttaat gctgcaattt tcagctcggt aatgtctttg atatactgta tcaattggtt
    22081 atctcttagg ttgaaaggaa ggaaagcttc gctgccttca acagcaaatt ccctgcggca
    22141 aacctgcata cagttacccc tgttggcgct gttaccacca ataaagctgc tgaacaagca
    22201 catacccgaa aaagagtagc acaatgcgcc gtgcacaaac aactccaaag gcatattggt
    22261 ttgttttacc atggttgcaa tctcggcacg agtaagttcc cgcgcaatga tagcccttga
    22321 aaaacccttc tgcaaagcat ggtttgcacc gcatgagttg tgtattccca tttgggtact
    22381 ggcgtgaatt tcgatacccg taaagtaatt ttttattagg aaatacacac cccagtcctg
    22441 aataataata gcatttggct tcagggcctt cagtagtttt aattgttcga gtattgattt
    22501 tatttcgcgg tttttcacaa gggtattcaa agtaacatat actttgattt ttctttctct
    22561 ggccaccttt ataactcctg aaagttccga tgaggtgaag ttggctgctc ttccgcgcgc
    22621 gttaaattgc tttaacccta gataaaccgc atcggcgcca ccttcgatgg cagcaaaaaa
    22681 cgcctcggta tttccggcag gcagcagcag ttcaggtttg cggtttttca tggggtaaaa
    22741 atacgaaata tgtgcgatta atttttgatg tgctgatttg ctgattttgt ttcttctcgc
    22801 aggcccgaag aaggtgggtt ccacccaaag tcgctaagac gcaagtggga aatgtagaat
    22861 acagcatgat ggacgtttca ttacatccga gtcctctgcg agagaatcgg gtggtgcgaa
    22921 aaaacgaata caagttaggt aatgtgatta tgaggattgt attccccaaa aaacaaagtt
    22981 tattttcaaa aacatttaca aatacataca agccggacac ctgtaccgta attaacaggc
    23041 aaaactttaa ggcattttta agcaactctt gatttgctga actattgaac tgggaacaca
    23101 aataataaaa gaaatatctc gtttaattgt taaagattgt tgatgcattg gaatggtgca
    23161 atacggtttt atccgtgtcc tttgcgagtg acttgatggg gtgatgaatg aaccggagca
    23221 ggagggaagt ataaaggata ttttagaacc aatttaattt cggacacaac gcacatagaa
    23281 tccccattgt ttgtttacgg ggttccggta caccgagggg tcatcataat atatggcgcg
    23341 cacccacgac gaaggctggc tgtcctcagt agccgaccag aggtatgtgt gcccccccat
    23401 ggctattgaa ttgccccatt ggtctctttg accaccgggc aggatgttaa aacctgaaga
    23461 attaaagctg ctgtggttga ttaaattatc atctgtccaa aggggtgcat taccggcaag
    23521 cgcacttcct gcatcatcgc cccgaaacca ttccagatcc cattccgggt cgccatacgg
    23581 ataggtggca tccacttcgc cttcgagttg tttccattca tcatcgctgg gaacgtgcca
    23641 gcctttgggg cagataccct gcaaaccact ggggttggtg ctgctgccca catatccgtc
    23701 catggcggcc caccaattat atattacccc atacgtcgcg taattttcat gtgctttagc
    23761 gagttccaca tcgctgccat tatatccata taccccatag gcccgttgaa tccatgagcc
    23821 aagcgattga aattcggcat cggtatgcac caacggtaaa taggccatat tctctgccat
    23881 ccagcactga gagcctatct gaacggtttg gtaggtgttt gcatcgcggt tatcggcaaa
    23941 agtacatcca catgaaaaca atgtttcctg tgtgagcatt aatgcctctg agcttccaca
    24001 tgcattgtaa gcccaaatat aaatagtgta ttccgtacca caatataatc cttccaaagt
    24061 aagtgatgta tccgttccaa tatcattaga ggtttcaaat tcacaggaag tgctccatgt
    24121 atatcgcgaa gcacctgcca caggaaacca attccagctg atctgatcta aaccggaaat
    24181 tatcgctccc tgttgaggtg tttcggggat ttctccatca atattaaagg tataatacat
    24241 actttcggaa ggaatatcgg taatttcttc agcagttcct tgtgcaaaac cggtaaacct
    24301 taactcgttc cctatacaat agttaaacca ggcttttcct gatttttttt tattatttcg
    24361 attataacca ttcactaacc ccatatatac aagcgacact ttattggctt gatggttagt
    24421 ttgaatcaaa tgcaagcatt gatggtggtt ggaagaacca acattgataa tatattgaaa
    24481 gctgcttcct gcaataagtg tgaatatgtg tgttccctga ttcaggtgga ttgttgaaac
    24541 caagagtttt ctgccggcga tgtcgaatat ctcaaatata aaattatccg gttttataac
    24601 ttccagatat atgtttgctt ctaagtcaaa tgggtttggt gcacattctg caacaaacga
    24661 agcattttgc aagctcatta catcggacat tcctgtaaag gaaaacgata ttaccgtgtc
    24721 gttccagtat aatgtagtat cgcagcccaa tgttgtgttt tctatcagaa cactatcgag
    24781 ctgagtatat tcacaggcat gatttgctgt aaacgaaaat gagatttgct gtgcgtttag
    24841 gctgatttgc aaacagagta tgaagcaggc aacgatgaat atagccctat ttaattttaa
    24901 attcatgata atattcgata tattacactt gtaacaaaga tagaatatat cttacatgtt
    24961 tccttttatt catattagaa catttcgacc aaggaattaa aaaaatgtat tgaaacactt
    25021 tttaatttta aaataatgca tttaattaat tcactacttt tacaaaaaag gataacatat
    25081 tttataaaag gtaattcacc catgaaaaaa aactttgtaa tacgcttaat tctcttggta
    25141 tcgcttttct cgctatgtat agttaccgga tgtaacgaag acgcaaattt tttcgagaca
    25201 atttttgcaa aaaaagtaaa aattaaaggt aaaatcgaca gatcgaaaag cacaaactct
    25261 gatgggacaa gagcttccga caacgattac acattagatc aggctgaaaa agtgttaata
    25321 ttttatggta ggaaatatga tttggtgaat attcacaacg atggcacttt tacggggcgt
    25381 gctcctctag gaagtgccac tgcagttgct tttctaaccc aggacaacca gtttatcgga
    25441 aatttattta caggtggaat aaattttatt ccgctggtgt cggataacgg agaccttact
    25501 gaaatagatc ttgatgaact ggtgcttgat ggagatcggg ttataccggc aaacgatcct
    25561 ctcgaaaatc aaataatact tagcgatgcc gaacttgagt ttatgcagga agttggggca
    25621 tactataaat cgttatcaaa aaacattgat atgaacaacg atggaactcc cgatgccatg
    25681 caacatggtg ttatcgacat tacaatctgt tccagttttt tcgccggcaa attcggggtt
    25741 aacgaatttg agcctgaatt ggggacattc gaccagtcat caatcaatta cacgattttt
    25801 atggaaggcc ctattgaatg ggtgtcatcg tccgacaata gtattcctga aaatgcaaca
    25861 ataacgggtc ctgaggaaaa tccctgcaac gatttgcgaa atgcggggaa ttcttattca
    25921 aatcatacca atttcaaagt aaattttgcc aaagggtcac aagatggtgt ttttcaacca
    25981 ttcccgcccg gtatttacac attgcatatc gacaataaag agtttacctt ttattattcc
    26041 agcctgaaca catttaatta ctgggtactg gctattccta cgctgattac aaatgatgca
    26101 ggggaagtta ccagcatccg gctggaatat cagtttccta acgggacaac aattgaacca
    26161 cgcaacctca taagtacggg aatcagtgtt gatatttccg gaatgaatta tgacaatatt
    26221 tattcataca tggcagagtc gaacagctct tccg
//
LOCUS       DAOOQP010000029        25604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2301_length_25604_cov_4.976672, whole genome shotgun sequence.
ACCESSION   DAOOQP010000029 DAOOQP010000000
VERSION     DAOOQP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_25604_cov_4.976672"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLK75_04865"
     CDS             <1..678
                     /locus_tag="PLK75_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOY38612.1"
                     /translation="LVLDEADRMLDMGFAKDIEQAGANGLELNMYIIPKDINQAPGVV
                     EQKYFEIIETLLKNISIPVAIKIGTYFSNLPWFVNQMKIRGINTFVLFNRFYEPDINI
                     DTLQMVSSSVFSKGSDIRGNLRWIGMINGLVKDVELSASTGIHDGAAVIKEILAGAQT
                     VQVCSVLYKKGMKVIESMIDEMQEWMDDHKYTSLSNFRGKLSYSNITDPETYERAQFI
                     KYFSSLE"
     gene            complement(681..1970)
                     /locus_tag="PLK75_04870"
     CDS             complement(681..1970)
                     /locus_tag="PLK75_04870"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOY38613.1"
                     /translation="MKRKSPLFILLAALLMFFSFTVYGTGFENLEYRNKVYVKFIHSV
                     RMTVGGWEVAYPVLAQDSDTYFEFSFDDIEADVKDYYYTVVHCKSDWTPSDLQYFEYA
                     SGFEENPVRNYQASRNTLLQYTHYSIELPNEDLQLNYSGNYLLVVYTNDDEGKKLVCS
                     YRFMLYENKMPITGGVYQPTGAEYSTGQKVSFIIDRRNYPLYNPFVELNVVVIQNNDW
                     STAKTKMEPMFVKSDELVFDIEDKQIFSGSNEFRYFSTRNLKILMDKIRSVEYHKPYY
                     FVELFSESSRYFDKYSSVEDINGCYVIKSEEETVIDNNTDADYTFVHFVLKTDMLTAN
                     RKIYVYGALTNWEFDEWSEMKYNKDLKGYELLLLLKQGYYNYRYVLAADGQFDNVHFE
                     GSFWQTENDYQIFVYHNDISAGYERLVGYNKISSRNR"
     gene            2061..3029
                     /locus_tag="PLK75_04875"
     CDS             2061..3029
                     /locus_tag="PLK75_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38614.1"
                     /translation="MKGYFCLAIFIIISLSCFSQGINNQAITVKNELTSDYSEVTGTQ
                     ISLLLPSGFVASDRFVGFEHKIAGTSIVITEVPGEVRKNFLAFGRVNLIQRGLLIEKE
                     ELYLINGFDALLQYGQQSAYGKMYRRWLLVIGDNQKTFLLNASAPAETSMDHAEAIKK
                     CLLSVVYQPAKKASSSERYDFTLDFKESGLKQVSILINSMIYTDDGILPPKTEAKVVM
                     MASRTKPAVPIKDKKVFASSLLDMLPVKWPNNEKPEPIPIELEGLSGYEVYGLAVNKD
                     NNRPQLVYELVLYNGDYYYLVSGLAFKYFEDNLDLFKSVARTFKLK"
     gene            3124..3813
                     /locus_tag="PLK75_04880"
     CDS             3124..3813
                     /locus_tag="PLK75_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOY38615.1"
                     /translation="MKKLALILFMFIAALIATGCASSRSYKHDMSLSSYGESYSETKS
                     LSGMSDNVTTPEPRKIIYSAYLTLAVKNMDTASNQIIRIAEQFNGYVQQSGTYSVTIR
                     VKSENLDMAVDDICQIGKVKSKNVAGQDVSEEYLDYQIRLENAEKARERYLELLSRAE
                     NVEAALKVEKELERLNETIELYKGKINRIDHLTEFSTITVSLQEKKKPGIIGYVGLGL
                     YYSVKWLFVRN"
     gene            3953..4876
                     /locus_tag="PLK75_04885"
     CDS             3953..4876
                     /locus_tag="PLK75_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY38616.1"
                     /translation="MKKVLIATDKPFAKDAVEAIKAVAVKAGYEIALLEKYTAKQELI
                     DACAKAEAVIIRSDIIDKEVIENSKNLKIVVRAGAGYDNVDLKSATEHGVVVMNTPGQ
                     NSNAVAELAFGMMLYMARGCFDGGTGTELRGKKLGIHAYGNVGKYVAEIAKGFKMDIY
                     AFDPFIKDEDIAKDGVKPVHSVEELYKTCQYVSLHIPANDKTKKSIGHGVVSMMPKNG
                     VLVNTARAEVIDEDGLIKIMEERPDIKYVSDIAPAAIATFQEKFAKRFFFTPKKMGAQ
                     TSEANNNAGIAAINQIVGFFEKGDKTFQVNK"
     gene            4907..6154
                     /locus_tag="PLK75_04890"
     CDS             4907..6154
                     /locus_tag="PLK75_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOY38617.1"
                     /translation="MAIVKPFKGLRPDKSVVENLTCLPYDVMNSEEAAKMAAGKPESL
                     LHVTHAEIDCPAGTDVHSETVYNKAVENFLNIQAKKWMAQDAEARFYIYAQTMNGKTQ
                     YGIVGCASCEDYRNGIIKKHELTRIEKEEDRMVLTRYTNANIEPVFFSYRAVPEIDTI
                     VNGIISNPPEYDFTSDDGFGHHFWVIADRETNRKIEKLFIEKVPFMYVADGHHRTAAA
                     ARIGMEKASKNTNHKGTEEYNFFMAVIFPDYQLNIIDYNRVIKDLNGMTESEFFGKLG
                     KYFDIGEMGTAIYKPSKLHEFSMYISGKWYKLNAKQGTYNDNDPIGVLDVTILSKLVF
                     DEILGIKDLRTSNRIDFVGGIRGLGELKRRVDNGEMVAAFALFPVSMKQLIDIADTGN
                     IMPPKTTWFEPKLRSGLVIHMLD"
     gene            6321..7382
                     /locus_tag="PLK75_04895"
     CDS             6321..7382
                     /locus_tag="PLK75_04895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY38618.1"
                     /translation="MIVIFITGWRKIITREPVTAIEKLPNVSVIVPFRNEESSLPLLL
                     QSLKQQHYTNFEVLLIDDHSTDNSVNAAKSVISSDTRFKIIKLDDEEGKKAAQREGVK
                     NAQSNFLLFTDADCTLGLNWIQHMVSAMITEKAAFVSSRIEYTETRGFFSKLLYLEFQ
                     SLIATGAAAISLNRASLCNGASMAVQKELYPALTEIEGAERSSGDDIFMMHHLKRKGG
                     TIVFNASPEASVKTSGPKTFGAFLRQRVRWASKSGSYHDFDSIFQGIIVLFMCIAIIT
                     SVLLAFSGVLSWWVPMAVFLTKTGCDFAFLLSYLQHYNKSKYLWYIVPVELLYPIYII
                     MVGILSLFWHPLWKGRKIR"
     gene            complement(7379..9562)
                     /gene="recQ"
                     /locus_tag="PLK75_04900"
     CDS             complement(7379..9562)
                     /gene="recQ"
                     /locus_tag="PLK75_04900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOY38619.1"
                     /translation="MQFEEKQIFSLLKKYFGFDTFKGPQREIIISLLEGSDSFVLMPT
                     GGGKSLCYQLPALMYPGTAIIISPLIALMKNQVDSMRGFSTEDGVAHFMNSSLSKAEI
                     LKVKEDLVAGKTKLLYVAPESLTKRENVDFLKSVQISFYAIDEAHCISEWGHDFRPEY
                     RRIRPIIDEIGKAPLIALTATATPKVQHDIQKNLDMLDAKVFKSSFRRHNLYYEVRPK
                     EHTTKEIIKYIKNNQGKSGIIYCLSRKKVEELAENLVVNDIKALPYHAGMDAATRSRN
                     QDMFLNEEADIIVATIAFGMGIDKPDVRFVIHYNIPKSLEGYYQETGRAGRDGGEGNC
                     IAFYSYDDIQKLEKFMQNKPVSEQEIGKQLLLETVSYAESSVCRVRQLLNYFGEVMEE
                     DCGNCDNCRNPKPKFEATEQLQILLEAIDLMKERCKGEHVANVLVGKSTAEINTFKHD
                     KLPIFGTGSDESVKFWKALIRQALIAQLIEKDIVNYGLLKITKVGREFLKKPHRFMMT
                     RDHDYEEQDDNSENVTGGTAAADMVLFSMLKDLRKTLAKQYNLPPFVIFGDPSLQDMA
                     IQYPITIEELTKISGVGVGKAQKYGKPFVELIHAHVVENEIERPQDMVVKSVVNKSGN
                     KVYIIKNIDLKVPLDDIAEAKGLEMIELIQEIEAIVNAGTRLNIDYYLNNIMDEDHIM
                     DIYDYFKDVADTESIEDALKELGENEFSEEEVRLVRIKFIAEMGN"
     gene            9744..10721
                     /locus_tag="PLK75_04905"
     CDS             9744..10721
                     /locus_tag="PLK75_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOY38620.1"
                     /translation="MNKKIKDFVLDVAKKTIILEIKSLENLPEYCDERFAEVVHLISK
                     SKGRLIVTGIGKSAIIGQKIVATLNSTGTPAIFMHAADAIHGDLGIVQEHDIVLCISK
                     SGNTPEIKVLIPLIKRLGNTLIGMVSDISSFLATEADYVIKATVAHEACPNNLAPTSS
                     TTAQLVMGDAMAVCLLELKGFTTEDFARYHPGGSIGRKLYMRVSELCKQNQVPKVSIH
                     DKVDVIIMEMTSKLLGATAVIDNNENLVGIITDGDLRRMLQKNLAVISELTAQDIMSR
                     NPKTIDESELAVKALRILEENSITQIIVTSRSNYLGMVHLHDLIREGVV"
     gene            10738..12387
                     /locus_tag="PLK75_04910"
     CDS             10738..12387
                     /locus_tag="PLK75_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOY38621.1"
                     /translation="MNKRFFFILIIALSLFYQNIKAQDNPIIPADKPKLVVGIVVEQM
                     RYDYLTRYWDKFGENGFKKLYIYGTNCQNANLNYSITQVSPGYATIATGAEPSEHGIV
                     SDYWYVPLTGKKVQSTEDKNTKCIGGSKNAEAFSPVNLLAPTLSDEIKLANRNQSKVI
                     SISMNSTGAALMGGFAADAAYWFDRETGNWVSGTYYMKVLPQWVDEINSKKQPENYLT
                     RTWIPMLPLSEYKESLNDTSIYEYGIDGLYKTFPYVYPKIRESVKKYELLTMVPEGNT
                     LTTDFAVNALFNEELGKDNITDFLFVSYSPAGVISKYFGPDAVETEDMYLRLDKEIAQ
                     LLSVLEETIGKNNVLIYLTSTSGISTVPSYLQDNRIPAGVFKQHYMTALLTSYIKALY
                     AEGTWIIDFANQQIYLNRLLIEDSKIPLNEMQDKIASFIINSEAVAYAISTHNMSNYA
                     ANDGILQKMKNSFHPKRSGDIMIALKPGWIYDVSYSADHGSAYNYDTHVPLLWYGWKV
                     KKNTVLDNVNITDIAPTISQMLQMPMPSHSSGQPIIELIKK"
     gene            12511..13287
                     /locus_tag="PLK75_04915"
     CDS             12511..13287
                     /locus_tag="PLK75_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOY38622.1"
                     /translation="MTYRCIEIEKAGATRILSLSRPEKKNAISFAMLHELDDAFRGIA
                     TDKSCNTVIIKGNGGLFSAGGDLKDIADIDESDAPITGIAVQEVFAKISILPQVVIAC
                     LDGLVAGGGAELALYCDIRIASSGFRLSLPEIKHGLIPGAGGIGMMSRFIGINNTLYY
                     LASGREMNADTAFRLGLVQEVCSVDELYDTCLSLANSINILQPEVVATAKRLAWMNLS
                     RPIDECLIAESREFGSLLNRFGKKQIQDFFSNKKKSNDNI"
     gene            13274..15136
                     /locus_tag="PLK75_04920"
     CDS             13274..15136
                     /locus_tag="PLK75_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY38623.1"
                     /translation="MTTFDSIIFSGLGYSLGKHRIENSEIENAIERGFLKGFDKARIE
                     ESGDYKSFAANHPEVSPFRYFAEQVMGFTTRYYVVPFPPSSARYSIAENSLDLCIQAV
                     ENAIACSGIQAEKVDAWMVSTATPHQQAPGLAETLKGYFTGFENKSQTMTLTSACVGF
                     NYNLERAVMFLRNHPEAKHIVVAHAEVMSELLVNETDFVPFVTFGDSAAAVVVSRITT
                     RQPEGVMNIVNYEDVQMLDFLGANKQRELYMNARMVKRRAVPNITSVFNELLTTSGWS
                     KSEVSHFVPHQTGNAIVHEVARNMGLLPDMMFQDVQQTMGNLSGASVPAVIAMLHESG
                     RLLPGNRLIASVAGLGGEFGGFSYIVPDFRGFNSEALHLKGKTALITGATGALGSAIA
                     AHLAKHGANLIVVFNSNTEKIKLLKDVLGKYKIKLHCIQCDFGKPDSMDKMIDEIKRE
                     YASIEYMVHTAAISGMVDRATKIPHSEMQRAYMVNKTAIQHITDKLMHLVSETVLFTG
                     SVAEDARFAGSSAYVASKNALHAYAAGISTKIQNTGARCIYYMPGIIDGGMGNLLNRE
                     QKAAGMMQIEQNEPLETGRIAERMVKSLYLPKVTNTRDSFENNLLVRKDGYFKY"
     gene            15168..16397
                     /locus_tag="PLK75_04925"
     CDS             15168..16397
                     /locus_tag="PLK75_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY38624.1"
                     /translation="MIINNKILLLKLADLFYNKKAELATANSTDIGTNLSVNSDNIER
                     GIAAMREWASGSASVDGKTKGGLAIYSCYSDPSFVLLGLCLPPAILANGNNTAIHIYI
                     PTVLRSYRSIFKQIVDESGLFPNITFVGSHRDFFEQSVTSPNIQSIVVFGDDWINNYL
                     GSIRDHEKTLIYFGPGNNTAVILDKSNLTQAIHSITRLTTILSGQAAVCFKRCIVSSN
                     VDFNDVLQLLSTQFKGVTTGTDVRTSVVTPIGFESIISTVAERVNEAAGNGAELIHFK
                     IKTLEDGVMVTPAIIANPHPNSDLWQKYHFAPVLSIVQCKPEEISSFIRHNDYQLAVS
                     VFGTDMKDEIIADLQADHAFVFDNCDFLDILRTNIGYRGHWGGFKNSAFTYSKESGYV
                     KNGGKFDLYSLITQENK"
     gene            16402..18207
                     /locus_tag="PLK75_04930"
     CDS             16402..18207
                     /locus_tag="PLK75_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY38625.1"
                     /translation="MESYIFSGIGHAEARFKITNNEIGTALHNYHLSGFSNDKVVSSR
                     NYQEFLKRQPGKTAFDYFTEEKMGFSVRHHVTPFPPTNKKIYFAESSLDLAVRAVRNA
                     LNNSGVHPSQVAAWFLSTVSPHEQAPGMAASMKSYLVSINNTTPAFTLTSGCAGFNIN
                     LERAVEYLKFHPESTHVVVVHSETMSSFLTNRIKFVPFVTFGDGAAAVVLSKVETPLP
                     QGLLNIVNYHDVSMVDFVGVDKRWDLFMDDNVIKDRAIINIPAAAESALRNTGWQTTD
                     IDFFVPHQTGNAILLPAIEKLGIPRSKLFTDAQMQYGNVSGATVPMSLSLLHQQNKLS
                     PGTKILSAMAGVGGNYGAFTYLVPEFSVSYPNKIIHEMEGKTAMVLGAQSGLGRAIAE
                     KLIEYGARVFAVITGNGALPHIQHNHLQYIFCDFSSDKDVNSLAIQLSASEIAIDYLI
                     QTSSELTQPENNNFFSPLEIIKASVPQKPQCIVAVGNISENVGFPDKHDYVSSLAALH
                     GSLASASGEFLSYGIRLIYYIPGFTEDDTTLQMDERSRFRLMMQAGQEQVLSINELSD
                     RIVQSLYILKVENTWSYYENAMVLRCDGFKPMVDL"
     gene            18352..18921
                     /locus_tag="PLK75_04935"
     CDS             18352..18921
                     /locus_tag="PLK75_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY38626.1"
                     /translation="MEKTAIFQYPVIDIIQKRWSPVLFDGKTISEQQANTLVEAAMWA
                     PSAYNEQPWRYIVAHKGSEKFDKILAALIEFNHAWAKEASLLVVSLAKKIYSHDGKPN
                     YHYMYDTGAANAFMINQATSMGIFSHSMGGIYYDKMRELFAIPEELEICSVIAFGYHS
                     EASGANEQLLQREQQPRMRKAMKDVVLHK"
     gene            complement(19618..20481)
                     /locus_tag="PLK75_04940"
     CDS             complement(19618..20481)
                     /locus_tag="PLK75_04940"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY38627.1"
                     /translation="MEIAVISGKGGTGKSSISAAFATLPEKVVLADCDVDAANLYILF
                     NPTQEEVQVYIGGQKAEINYSLCTNCKLCIDYCRFGAISLLNDKVIISDTSCDGCKLC
                     SRICPASAISMIDSNKSRMYAGTFRNGKMVYGRLAPGEENSGKLVNIVRDKAKTIAKA
                     ENIETIIIDGPPGIGCPVISTITGVDHVVIVTEPTISGIHDLKRTIEITTKFNLKTWV
                     IINKYDLNSEMTNRIEDYCNSINISIVGKLLFNPHVVEAMVNCKSVVEYAPKSDFATG
                     IKSAYNIITNK"
     gene            complement(20462..21328)
                     /locus_tag="PLK75_04945"
     CDS             complement(20462..21328)
                     /locus_tag="PLK75_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY38628.1"
                     /translation="MKIAIASGKGGTGKTLISTNLFYVLQQHNINISLVDCDAEEPND
                     LVFFIGEKEKSFEVNQKVPVIDESKCTFCGKCHEYCNYNAIFILPPAKIIKVIEDLCH
                     GCGACTVACKLDAISEKDVSLGTVSCFSNTEKSTIIEAKMKVGVFSPVSIIKEAIKEA
                     GNNGIILLDSPPGTSCPFIQTVVGADFVILVTEPTPFGLSDLKQSVETLKTMNKTCGV
                     IINRAGLGDNEIYDYLKKIQIPLLMEIPFDKEIASFYSKGELFVKYKPEWEQHFISMY
                     NSIVEEYGNSRN"
     gene            complement(21339..21782)
                     /locus_tag="PLK75_04950"
     CDS             complement(21339..21782)
                     /locus_tag="PLK75_04950"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOY38629.1"
                     /translation="MQKIKIGIIICNRYHTCAGGKCFRALQNREGAFYEYKDKEVEIA
                     AYTTCGGCPGGNIEYAPEEMKKNGVTVIHFATGMVVGYPPCPRIDYFKKFIEDKYNLK
                     VVIGTHPIPQKYFLPHNKLKTWQSKFWQEAIMPTIADEQTRLEYD"
     gene            complement(21788..22681)
                     /locus_tag="PLK75_04955"
     CDS             complement(21788..22681)
                     /locus_tag="PLK75_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOY38630.1"
                     /translation="MMKIMNTGTVNIKPSYIVDVRCEKCFLKSYKRLFEKFNVNEYLQ
                     QEFIAFFKEKISTSNHLSSPEFQPLLHNKFCNLIKVSDPYSEEKKLSNDIALGLYAEW
                     KPKVLVSENPFVLALKLAIAANIMDYGANNSFDIHETIETVFTSSFAIDHSKQLQKLI
                     KQAKRILYLGDNAGEIVFDRLFIETMMHNDIIYAVKEYPVLNDVTIEDAEYVGMNLVA
                     DVISNGYNAPSTVLHKCSKEFIDVYNSADLIISKGQGNYEGLMNENNSRIFFLMMVKC
                     DVIAEKLNVEKGSFIVYNHSE"
     gene            complement(22644..23015)
                     /locus_tag="PLK75_04960"
     CDS             complement(22644..23015)
                     /locus_tag="PLK75_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOY38631.1"
                     /translation="MKKIIAIPLENGVLCAHFGHCQTFALVNVENNKITEVKEAVPPE
                     HVPGLYPKWIAQFGVTDVIAGGMGQQAIMLFNQQNINAFVGAPVKSAKELVTDFLENK
                     LSLSANYCNHDENHEHGNCQH"
     gene            complement(23020..23730)
                     /locus_tag="PLK75_04965"
     CDS             complement(23020..23730)
                     /locus_tag="PLK75_04965"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOY38632.1"
                     /translation="MENQEKNAMPRIGENAPEFKAVTTQGQINFPSDYKGSWVILFSH
                     PADFTPVCTSEFMTFATLEPKFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKN
                     VEVTFPLIEDITMEVAQKYGMMMPGESNTKAVRAVFVIDPKGIIRTIIYYPLSLGRNF
                     DELYRVVLALQTADEFSVATPADWRPGDEVIVPTAGSCGVAKERMESQDEHTKCYDWF
                     FCTKKIDKDTVLKKILKK"
     gene            complement(23808..24047)
                     /locus_tag="PLK75_04970"
     CDS             complement(23808..24047)
                     /locus_tag="PLK75_04970"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOY38633.1"
                     /translation="MPGFNQTGPNGQGPMTGRRMGRCTNFGASLKNQSATIVNENPNE
                     NNPKNVQGRGFGYAKGFGRGRGRGMGRQNRSRSGQ"
     gene            complement(24121..24444)
                     /locus_tag="PLK75_04975"
     CDS             complement(24121..24444)
                     /locus_tag="PLK75_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOY38634.1"
                     /translation="MKVAITSSGNSLESTIDQRFGRCAYFVVYDTESKSTEFIPNPNK
                     DAEEGAGPASVQLVASRNVNKIVSGEFGMKIKPLLDSLKIQMIVLKEPDKKIKDILEM
                     LHHKN"
     gene            complement(24446..25039)
                     /locus_tag="PLK75_04980"
     CDS             complement(24446..25039)
                     /locus_tag="PLK75_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOY38635.1"
                     /translation="MSPRIKALRKVSNPPVIKGFKPYGPESEKQKTEPVNLLYEEYEA
                     IRLSDYDILNHHQASVMMGVSRPTFTRIYASALQKIAKAFVEGRQISIEGGKVYFDSD
                     WYDCKKCGCYFNNPEKEKSVNNCPLCGSQRVDKFDYENTHEDIADKPCYDMCICPECG
                     FEQEHQYGQPCSKQICPHCKSFMKRKGSPNCRNFKNQ"
     gene            25128..25532
                     /locus_tag="PLK75_04985"
     CDS             25128..25532
                     /locus_tag="PLK75_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOY38636.1"
                     /translation="MDDRIMKSEIRQFKVIFPNTLNDHDTLFGGIAMQWMDEVAYITA
                     IRFSKKKVVTVSAEKVQFLLPMKSGTIAEIIGKVTKVKNVKIEIQVEIYIDDMYSDNR
                     QKAVDALFTFAAINDENKPIPINTKEYSSQEV"
BASE COUNT         7816 a         5135 c         4999 g         7654 t
ORIGIN      
        1 ttagttctcg atgaagcaga ccgtatgtta gacatgggtt ttgcaaaaga tattgagcaa
       61 gccggagcca atggacttga attgaacatg tatattattc ctaaagacat taatcaggca
      121 cccggagttg ttgaacagaa atatttcgaa ataatagaga ctttgctaaa aaacatatct
      181 attcccgttg caattaaaat cggaacctat ttcagcaacc ttccgtggtt tgtaaatcaa
      241 atgaaaatca ggggtatcaa cacttttgtt ctgtttaacc gcttctatga acccgatatt
      301 aatatcgaca cgctccaaat ggtgtcgtcg agtgtattca gcaaaggatc cgatattcgc
      361 ggtaacctgc gctggattgg gatgattaat ggtctggtaa aagatgttga gctgtcggcc
      421 tccacaggta ttcacgacgg agctgcagtt attaaagaaa tacttgccgg agctcaaacc
      481 gttcaggttt gctcggtact ttacaaaaag ggcatgaaag tgattgagtc aatgattgat
      541 gaaatgcaag aatggatgga tgatcataaa tataccagcc taagcaactt cagagggaaa
      601 ctctcctact ccaacattac tgaccctgag acctatgaga gggcacaatt cattaaatac
      661 ttcagctccc tggaatagat ttatcggttg cgtgaactga ttttattgta accaaccaga
      721 cgttcgtaac cagcgcttat atcgttgtga tacacaaata tttggtaatc gttttcagtt
      781 tgccaaaaac ttccttcaaa gtgcacatta tcgaattgtc catcggcggc aagtacataa
      841 cgatagttgt agtacccctg ctttagcagt aacagcaatt cgtacccctt taagtcctta
      901 ttatatttca tctccgacca ttcgtcaaat tcccagtttg taagcgcacc atacacataa
      961 atctttcggt ttgcagtgag catgtccgtc tttaacacaa aatgtacaaa tgtgtaatcg
     1021 gcatcagtgt tattgtcgat tacggtttct tcctccgatt taataacata gcaaccatta
     1081 atatcctcca ctgagctgta cttatcgaaa taccgcgagc tttcactgaa taattccaca
     1141 aaataataag gtttatgata ctcaaccgat cttattttgt ccatcaatat cttcaggttg
     1201 cgtgtggaga agtagcgaaa ttcgtttgaa ccggaaaaaa tctgcttatc ttcaatatca
     1261 aacaccagct catccgattt aacaaacata ggctccattt ttgttttggc tgtactccaa
     1321 tcattgtttt gaattacaac tacatttagt tcaacaaaag gattatacag agggtagttt
     1381 ctccggtcga tgatgaaact tactttttga cctgtactat attctgcacc ggtaggctga
     1441 tagacacctc cggtaattgg cattttattt tcgtacagca taaacctata cgaacacacc
     1501 agctttttac cttcatcatc attggtataa accaccagga ggtaattccc cgaataattc
     1561 aattgcaggt cttcattggg caattcaatg gaatagtgtg tgtattgcag aagagtattc
     1621 cgcgaggcct gatagttgcg taccgggttc tcctcaaaac ctgaagcata ttcgaaatac
     1681 tgcaaatcgg atggcgtcca gtcgctctta cagtgtacga ccgtataata ataatcctta
     1741 acatcagctt caatatcatc gaacgaaaac tcaaaatacg tatcagaatc ttgcgccaga
     1801 acagggtaag ctacctccca cccaccaaca gtcatcctga ccgaatgaat aaattttacg
     1861 taaactttgt tgcggtattc caggttttcg aaccccgttc catataccgt aaaagaaaaa
     1921 aacattaata aagctgctaa tagaataaat agcggagatt tacgtttcat atcagttggc
     1981 cggtttagca attggtacat ttattgaaat taaataactg cttaataaat aattgcgtaa
     2041 ttttacaaaa aaataagaca atgaaggggt atttttgctt ggcaattttc atcataattt
     2101 cgctgtcgtg tttttcacaa ggcatcaata atcaagccat taccgttaag aacgagttaa
     2161 ccagcgatta ctcagaagta acaggaacgc agataagcct tcttctccct tccggttttg
     2221 ttgcttccga caggtttgtg gggttcgagc ataagattgc cggaacatcc atagtaatca
     2281 ctgaggttcc gggtgaggta agaaagaatt ttcttgcttt tggaagagtg aatcttatcc
     2341 agcgcgggct gctaatcgaa aaggaggaat tatatttaat aaacggcttt gatgctttgc
     2401 tgcagtacgg gcaacaaagc gcttacggaa agatgtaccg ccgttggctg ctggttattg
     2461 gcgacaatca gaaaactttt cttctgaatg catccgctcc tgccgaaacg agcatggatc
     2521 atgccgaggc tattaaaaag tgccttctat cagttgtgta tcaacctgca aaaaaggcat
     2581 catcttccga acgctacgac ttcacgcttg actttaaaga atctgggtta aagcaggtta
     2641 gcatattaat taactcgatg atttataccg atgatggcat tcttccaccg aaaactgaag
     2701 ccaaagttgt gatgatggcc agtcgcacca aacccgctgt acctatcaaa gacaaaaaag
     2761 tatttgcatc atcccttctt gatatgcttc ctgtaaaatg gcccaataat gagaaacctg
     2821 aacccatacc cattgagctt gaaggtctga gcggctacga agtgtacggg ctggctgtga
     2881 acaaagataa taaccgcccg caactggtgt atgagttggt gttgtacaat ggcgattact
     2941 attatctggt aagcggactg gcattcaagt acttcgaaga caatctggac ttatttaaaa
     3001 gcgttgcaag gacgtttaag cttaagtaat taatatacag aaacaatttc ggtatttaag
     3061 acgacccaat tggcaggtaa aagaaaatag atttatagtt aaatacgatt aaaccttcaa
     3121 actatgaaaa aactggcatt aatattattt atgtttatcg ctgcactcat tgccaccggc
     3181 tgtgcaagtt cgcgtagtta taaacatgac atgagtttga gtagctacgg ggaaagctat
     3241 tcagaaacaa aatcactttc aggcatgagc gataatgtaa ccactcccga accaagaaaa
     3301 attatttatt cagcttatct tacacttgca gtaaaaaata tggacacagc aagcaatcag
     3361 ataattagaa ttgctgagca gttcaatggg tacgtacaac agagcggaac ctattcggtt
     3421 accattcgtg taaaaagcga aaacctcgac atggctgttg atgatatttg ccaaattgga
     3481 aaagtaaaga gtaaaaatgt ggcaggacag gatgtgagcg aagagtatct ggattaccag
     3541 atcaggcttg agaatgccga aaaagcccgt gagcgctatc ttgagctgct ttccagggcc
     3601 gaaaacgtgg aagctgcgct taaagtggaa aaagaactgg aaaggttaaa cgaaaccatt
     3661 gaactgtata agggcaaaat caaccgtatt gaccacctta ccgaattctc aaccattacc
     3721 gtcagcctcc aggaaaagaa aaagccaggg attatcggat atgttgggtt gggattgtat
     3781 tattcggtaa aatggctgtt tgttcgcaac taacacaaaa tcaggttttt tacaataaat
     3841 gagtatacgg catttatgct gtaaaatatt ttattacaag aagtacatcc gacttattta
     3901 ctaattttgt ttcagttaaa acaaaattaa gtttcatcaa aatacattga ttatgaaaaa
     3961 agtattgatt gcaaccgaca agccattcgc aaaagacgct gtcgaagcta ttaaagctgt
     4021 tgcggtaaaa gcaggttacg aaattgcgct gctcgaaaaa tatactgcca agcaggaatt
     4081 gatcgacgct tgcgcaaaag ctgaagctgt aattatcaga agcgacatta tcgataaaga
     4141 ggtaattgaa aattcgaaga accttaaaat tgttgtacgc gcaggtgccg gctacgataa
     4201 tgtggatttg aaatctgcaa ccgagcatgg cgttgtggta atgaatactc cggggcagaa
     4261 ttcgaatgct gttgccgaac tcgcttttgg tatgatgctt tatatggcac gcggatgctt
     4321 cgatggtggc acaggaaccg aattaagagg taaaaaatta gggatacatg cttacggaaa
     4381 tgtaggaaaa tatgtcgctg aaattgcaaa aggctttaaa atggatatct atgctttcga
     4441 tccgtttatt aaagacgaag atattgctaa agacggtgta aaacccgtgc attcggttga
     4501 agaattgtat aaaacctgtc aatacgtttc gttgcatata cctgcaaacg ataaaaccaa
     4561 aaaatcgatt ggtcacggtg tagtaagcat gatgccgaaa aatggggttt tagttaatac
     4621 tgcccgcgct gaagtaatcg acgaggacgg attgattaaa ataatggaag aacgcccaga
     4681 cataaaatat gtttctgaca ttgcccctgc tgccatagca acttttcagg aaaaatttgc
     4741 aaaacgcttc tttttcacac cgaaaaaaat gggtgctcaa acatcggaag ccaacaacaa
     4801 tgccggaatt gccgccatca accaaatcgt cggctttttc gaaaaaggcg ataaaacttt
     4861 tcaggtgaac aaataaacta ataattcatt ttaatatttt ttaaagatgg caattgtaaa
     4921 accatttaaa ggacttcgtc ctgataaatc tgttgttgag aatctgacct gtctgcctta
     4981 tgatgttatg aacagcgaag aagcggctaa aatggctgcc ggtaaacccg aatcgctgct
     5041 gcatgtaacc catgccgaaa tcgattgtcc tgcaggaacc gatgtacaca gcgaaacagt
     5101 atataacaaa gccgttgaga actttctgaa cattcaggca aagaaatgga tggctcagga
     5161 cgctgaagcc cgcttctata tttatgcaca aaccatgaac ggcaagacac aatatggcat
     5221 tgtaggctgt gcatcatgcg aagattaccg caacggtatc atcaaaaaac acgaactcac
     5281 ccgtatcgaa aaagaagaag accgaatggt gcttacacgt tatacaaatg cgaatattga
     5341 gcctgtattc ttctcgtatc gtgctgttcc cgaaatcgat actattgtga atggtattat
     5401 cagcaacccg cccgaatacg atttcacttc cgatgatggc tttggtcacc atttctgggt
     5461 tatcgccgac agagaaacca accgcaagat tgaaaaacta ttcattgaaa aagtgccgtt
     5521 catgtatgta gccgacggtc accaccgaac tgctgctgca gcaagaattg gcatggagaa
     5581 agcatcgaaa aatacaaacc acaaaggcac cgaagaatac aactttttca tggccgttat
     5641 tttccctgat tatcagctga atattatcga ctataaccgt gttatcaaag acctgaacgg
     5701 catgaccgaa agcgagtttt tcggaaagct cggtaagtat ttcgacattg gtgaaatggg
     5761 caccgcgata tataaaccct cgaagcttca tgaattcagt atgtatatca gcggcaagtg
     5821 gtataaactc aatgcaaagc agggaacata caacgacaac gatcccattg gagtactcga
     5881 tgttaccatt ctttcgaaac ttgttttcga cgaaattctg ggtataaaag acctgcgcac
     5941 aagtaacagg atcgattttg taggcggtat cagaggcctg ggtgaactga agagacgtgt
     6001 cgacaatggc gaaatggtgg ctgcctttgc ccttttcccg gtttcgatga aacaactgat
     6061 tgatattgcc gatacaggaa atatcatgcc acccaaaacc acatggtttg agcccaagtt
     6121 gcggtctggc ttggttattc atatgcttga ttagcatcct ttcgcgatat actgtaaagc
     6181 agttgccacg gtgactgctt tttttgttat ttcacaattt tggcatttga ccaaaaattc
     6241 gttttcttta ctaaaaacaa acaaacttgg aactcatcgt tataatacac cttttatctt
     6301 tttcgctttt atattttacg atgattgtaa tttttattac aggatggcga aaaatcatta
     6361 cacgagagcc tgttacagca attgaaaagt tacccaatgt ttccgttatt gtgccattcc
     6421 gtaacgaaga atccagctta cccctgttac tgcagtcgct gaaacagcaa cattatacaa
     6481 atttcgaagt tttgcttatc gatgaccata gcactgataa ttcggtaaat gctgctaaaa
     6541 gtgttatttc ttcagacaca aggtttaaaa tcatcaaact tgatgatgaa gaagggaaaa
     6601 aggcagcaca acgcgaaggt gtaaaaaatg cacaatccaa tttcctgctc tttacagatg
     6661 ccgattgtac gttggggctg aactggatac agcacatggt atcggcaatg attaccgaaa
     6721 aagctgcttt tgtgagctcg cggattgaat ataccgaaac ccgcggtttt ttttcaaagc
     6781 ttctgtacct tgaatttcaa agcctcatcg ccacaggggc cgctgcaata tcgctgaaca
     6841 gagccagcct ttgtaatgga gccagtatgg ctgtgcagaa agaactatac cctgccctca
     6901 cagaaattga aggtgctgaa cgcagttcgg gtgacgatat ttttatgatg catcatctta
     6961 aacgaaaagg cggaacaatt gttttcaacg cttcgccgga ggcatcagtt aaaacatcag
     7021 gaccaaagac atttggggca tttctgcggc aacgggttcg ctgggcatcg aaatcgggtt
     7081 cgtatcacga tttcgacagt atttttcagg ggataattgt gctgttcatg tgtatcgcta
     7141 taataacaag cgttttactc gcattttcgg gggtattatc atggtgggta cctatggccg
     7201 tgtttctgac taagacagga tgcgattttg cttttttatt gagctatctg caacattaca
     7261 ataaaagcaa gtatttgtgg tatattgtac ctgttgaact tttataccct atctatatta
     7321 ttatggtagg tattctgagt ttattctggc atccgttatg gaaaggaagg aaaatacgtt
     7381 aattacccat ttcggctata aacttaattc ttaccagacg cacttcttct tccgaaaact
     7441 cattttcacc cagttctttt aaagcatctt cgatcgattc ggtgtcggca acatctttga
     7501 aataatcgta aatatccata atatggtcct catccataat gttgttcagg tagtagtcga
     7561 tattaagacg cgttcccgca ttcacaattg cctcaatctc ctgaatgagt tcaatcattt
     7621 cgaggccttt ggcttcggca atatcgtcga ggggaacttt taagtcgatg ttcttaatga
     7681 tgtaaacctt atttcccgac ttatttacca ccgatttcac caccatgtcc tgcgggcgtt
     7741 caatttcgtt ctcaaccaca tgggcatgaa taagctccac gaatggtttt ccgtatttct
     7801 gggctttacc cactcccact cccgaaatct tggtaagctc ctcgatggta atagggtatt
     7861 gaattgccat atcctgcagc gaagggtctc caaaaatcac aaatggaggc aaattgtatt
     7921 gttttgcgag tgtctttctt aaatctttca acattgagaa cagcaccata tcggcagcag
     7981 ccgtgccacc ggtaacattt tcgctgttat cgtcctgctc ttcataatcg tgatcgcggg
     8041 tcatcatgaa ccggtggggt ttcttgagga attccctgcc gacttttgta attttcagca
     8101 ggccgtagtt cacaatatcc ttttcgataa gctgtgcaat cagagcctga cgtatcagag
     8161 ctttccagaa cttgacagat tcgtcggaac cggtaccgaa aataggtagt ttatcgtgtt
     8221 taaaagtatt gatctcagca gtcgattttc caacaagtac gtttgccacg tgttccccct
     8281 tacagcgttc tttcattaga tcgattgctt ccagcagtat ctgcaattgc tctgtagctt
     8341 cgaattttgg ttttgggtta cggcagttat cgcagttgcc gcaatcctct tccatcactt
     8401 cgccgaaata attcagcaat tgccttaccc tgcataccga cgactctgcg taggataccg
     8461 tttcgagcag cagttgctta ccgatttcct gttcgcttac gggtttgttc tgcatgaatt
     8521 tttcaagctt ctggatatcg tcgtaactgt aaaaagcgat acagtttcct tcgcctccat
     8581 cgcgcccggc acgtccggtt tcctgataat atccttcaag gctcttagga atgttgtaat
     8641 gaataacaaa acgcacatcg ggtttgtcga ttcccattcc aaatgcaata gtagcaacaa
     8701 ttatgtcggc ttcttcattt aaaaacatat cctgattccg cgaacgggta gcagcatcca
     8761 taccggcatg atacggcaaa gccttaatat cgtttaccac caggttctcg gctagctctt
     8821 ccactttttt acgactcagg caataaatga tacctgattt tccctggttg ttcttaatat
     8881 atttgatgat ttccttggtc gtatgctctt tcgggcgaac ttcgtagtag agattatgac
     8941 ggcggaacga cgatttaaat accttggcat cgagcatatc aaggtttttt tgaatatcgt
     9001 gctgaacctt cggggtagcg gtagcggtta gggcaatcaa gggagcttta ccaatctcat
     9061 caataatggg tcgtatacgc cggtactctg gtcggaagtc gtgtccccat tccgaaatgc
     9121 aatgcgcttc gtcgatggca taaaaagata tttgaacact tttcaaaaaa tcgacatttt
     9181 cgcgtttcgt cagcgattcg ggagcaacat acaggagttt tgtttttccg gcaaccagat
     9241 cttcctttac cttaagtatt tcggctttac tgagtgatga attcataaaa tgagccacac
     9301 catcttcggt gctgaatcca cgcatcgaat ccacctgatt tttcatcagt gcaataagcg
     9361 gagatattat aatggccgtc cccggataca tgagagccgg taactgatag cacaacgatt
     9421 taccaccccc ggttggcatc agcacaaaag agtcggaccc ttccaaaagg ctgataatga
     9481 tttctctttg cggcccttta aaagtatcga aaccaaaata ctttttcagc agactaaaaa
     9541 tttgcttttc ttcgaattgc attatttctc agatgatgtt ctaattcaaa gttaatataa
     9601 atctgttcca ttgataaaat tttcatgctt ttttttagaa ttattttttt aataattata
     9661 aataccagat tgttgattaa atcatactat tgaagcaata atcgtttgtc gataatgtta
     9721 tttttgcagc aagccttacg aaaatgaata aaaaaataaa agatttcgta ctcgatgtcg
     9781 caaaaaaaac cattatcctg gaaattaaat cgcttgagaa cctgcccgaa tactgcgatg
     9841 aaagatttgc cgaggtggtg catctcattt caaaatcaaa aggcagacta attgttactg
     9901 ggattggaaa aagtgcgatt atcgggcaaa aaattgttgc aacacttaac agcacaggca
     9961 cacccgcaat atttatgcat gctgctgacg caatccatgg cgatcttggc attgttcagg
    10021 aacatgacat tgtgctatgc atttcgaaaa gtggtaatac tcccgaaata aaagtactta
    10081 ttccgcttat taaacggctt ggtaacacac tcataggcat ggtgtcggat atcagttcat
    10141 ttctggcaac ggaagccgat tatgttataa aagccacagt tgcacatgaa gcctgtccaa
    10201 ataaccttgc acccacaagc agtactaccg ctcagctggt aatgggcgat gctatggcgg
    10261 tttgcctcct cgagctgaaa ggctttacaa cagaagattt tgccagatat catcccggag
    10321 gatcaatcgg acgtaagtta tatatgcggg ttagcgaact atgtaaacaa aatcaggtgc
    10381 cgaaggtaag catacacgat aaggtagatg tcataatcat ggaaatgacc agtaagctgt
    10441 tgggcgcgac tgcagtaatt gataacaacg aaaatctggt tggaatcatt accgacggcg
    10501 atttgagaag aatgcttcaa aagaatctgg cagttataag tgagctgaca gcacaggata
    10561 tcatgtcgcg aaaccctaaa accatcgacg aatcggagct ggcagtaaaa gctttgagaa
    10621 tactcgaaga aaacagtatt acacagataa ttgttaccag cagaagcaat tatctgggga
    10681 tggtgcatct gcacgatctg atcagagaag gtgttgtata acgcagaaga tatttttatg
    10741 aacaaaagat tttttttcat tctcattatt gcattatcat tgttttatca aaacattaag
    10801 gcacaggaca atccgataat acccgccgat aaacccaaat tagttgttgg cattgtggtt
    10861 gaacagatgc gttacgatta cctgacacgt tactgggaca aattcggaga aaatggcttt
    10921 aagaagctct atatttatgg aaccaactgc cagaatgcca atttaaacta cagtattaca
    10981 caagtttcgc ccggttatgc aaccattgca accggtgccg agccttctga acatggaatt
    11041 gtttcagatt actggtatgt gccgcttacc ggtaaaaaag tgcaaagcac cgaagacaaa
    11101 aacacaaaat gtattggtgg ctccaaaaat gctgaagcgt tttcgcctgt aaatctgctg
    11161 gctcccacgc tcagcgatga aataaaactg gcaaaccgga atcagtcgaa agtaatttcc
    11221 atttcgatga acagcaccgg cgcagcgtta atgggcggct ttgctgctga tgccgcctac
    11281 tggttcgacc gtgaaaccgg aaactgggtt tcgggcacgt attatatgaa agttttaccc
    11341 caatgggtcg atgaaataaa cagtaaaaaa cagcctgaaa actatcttac ccgtacctgg
    11401 attcccatgc ttcctctaag tgaatataag gaatcgctca atgatacaag tatttatgag
    11461 tatggcatcg acggccttta taaaactttc ccgtatgtat atcctaaaat cagagaatcc
    11521 gtaaaaaaat acgaactgct taccatggtt cccgaaggga atacgcttac aaccgatttt
    11581 gctgtaaatg cccttttcaa cgaagaactg ggcaaagata atataaccga cttccttttt
    11641 gtgagctatt ctccagccgg agttataagt aaatacttcg gccctgatgc tgtagaaact
    11701 gaagatatgt acttacgact tgataaagaa attgcacagc ttttatcggt gctcgaagaa
    11761 accatcggta aaaacaacgt acttatctat ctcacttcaa ccagcggaat aagcaccgtg
    11821 ccttcatacc tgcaagataa ccgtatacct gccggggtgt ttaaacagca ctatatgaca
    11881 gctttgctca cgtcctatat taaagctctg tatgccgaag gtacctggat cattgatttt
    11941 gcaaatcagc aaatctatct gaaccggctg ctcatagaag attccaaaat tccactgaac
    12001 gaaatgcagg ataaaattgc ctcatttata atcaacagcg aagctgtagc ctatgccata
    12061 agtacccata acatgtcgaa ctatgcagca aatgacggga tattgcagaa aatgaaaaat
    12121 tcatttcacc caaagcgaag cggcgacata atgatagcgc tgaaacccgg ctggatctac
    12181 gacgtaagct attcggccga ccatggttct gcgtataatt atgatactca tgttccattg
    12241 ctttggtacg gctggaaagt gaagaagaat accgtgcttg ataacgtaaa tataaccgac
    12301 atcgcaccaa ctatatctca aatgctgcag atgccaatgc cctctcattc cagtggacaa
    12361 ccaataattg aactgataaa aaaataaaac tattccctga aatgacagga cggttgtttc
    12421 gctgaaatat tttaagtaat tgggcgttct gtttgagcac ttcttttcgg aattgctgat
    12481 aatatgttaa ttttaccaca aatataaaat atgacttacc gttgcattga aatcgagaaa
    12541 gccggagcaa cccgtatact gagtttatcg aggcctgaaa agaaaaatgc catcagtttt
    12601 gctatgctcc atgaacttga tgatgctttt cgtggtattg ccactgataa aagctgcaac
    12661 actgtaataa taaaaggtaa cggagggcta ttttcagcag ggggcgatct gaaagacatt
    12721 gcagatatag atgaatccga cgctcccatt accggaatag ctgttcagga agtatttgca
    12781 aaaatttcaa ttttgcctca ggtggtaatc gcctgtctcg atgggttggt ggccggtgga
    12841 ggtgcagagt tggccttata ttgcgacata agaattgcat catcaggttt cagactatca
    12901 ttacctgaga taaaacatgg tttgatacct ggtgccggag gtattggcat gatgtcgcgc
    12961 tttattggga ttaacaatac gctatattac ctcgcttcag gaagagaaat gaatgcagac
    13021 actgcatttc gactcggact ggttcaggaa gtgtgctccg tcgatgaatt atacgacaca
    13081 tgtttatctc ttgcaaatag cataaacatt ttgcaaccag aagtagttgc tactgcaaag
    13141 cgtctggcat ggatgaatct cagccggcct atcgacgaat gcctcattgc tgaatcaaga
    13201 gaatttggca gtctgcttaa ccgattcgga aaaaagcaaa ttcaggattt cttcagtaat
    13261 aagaaaaaaa gtaatgacaa catttgatag cattatattc agcggattgg gctattcgct
    13321 gggaaaacat cgcatagaaa attcagaaat tgaaaacgct atcgaaagag ggtttctgaa
    13381 aggatttgac aaagcccgaa ttgaggaatc aggcgactat aagagttttg ctgccaatca
    13441 ccccgaagta tcgccattcc gctactttgc cgagcaagta atgggtttta caacccgtta
    13501 ctatgtagtg ccttttccac cctcttctgc acgttatagt attgcagaga atagccttga
    13561 cttatgtatt caggctgttg aaaatgcaat tgcatgttct ggcattcaag ctgaaaaagt
    13621 agatgcatgg atggtttcta ccgcaacacc gcatcaacag gcaccgggtc ttgctgaaac
    13681 attaaaagga tattttaccg ggtttgagaa taaatctcag acaatgacac taacttcggc
    13741 ctgtgttgga ttcaactata atctggagcg tgctgtaatg tttctgcgta atcaccctga
    13801 agcgaagcat attgttgttg cacatgccga agtaatgtcg gaactacttg tgaacgaaac
    13861 cgattttgtg ccctttgtaa ctttcggcga cagtgcggca gccgtggttg ttagtcgcat
    13921 tacaacccgc caacccgaag gagtaatgaa catcgttaat tatgaagatg tgcagatgct
    13981 cgattttttg ggagcaaata agcaaaggga gctatatatg aatgcccgca tggtaaaacg
    14041 cagggccgta ccgaatatta cttcagtttt caatgagtta cttacaacat cggggtggag
    14101 caaatcggaa gtcagtcatt ttgtaccaca ccaaacaggg aacgctattg tgcatgaagt
    14161 ggcacgcaat atgggtcttt tgcctgatat gatgtttcag gatgtgcagc aaacaatggg
    14221 gaacctttcg ggcgcatcgg taccggcagt cattgcaatg cttcatgaat cgggaagact
    14281 cttgccaggc aacaggctta ttgcttccgt ggcgggcctg ggcggggaat tcggaggatt
    14341 cagttacatt gttcccgatt tcaggggatt caactccgaa gcacttcacc ttaaaggcaa
    14401 aacggcactt ataaccggtg ccacaggtgc tcttggctca gccatagctg ctcatcttgc
    14461 aaagcatgga gcaaacctga tagttgtatt taatagtaat accgaaaaga ttaagctttt
    14521 gaaggatgtg ctcggtaaat acaaaataaa acttcattgc attcaatgcg atttcggcaa
    14581 accggattca atggacaaaa tgatcgatga aattaagaga gaatatgctt ccatcgagta
    14641 tatggtacat acggcggcaa tatcgggaat ggttgaccgt gctactaaaa taccacattc
    14701 cgaaatgcag cgtgcctaca tggtgaataa aaccgctatc caacatatta ccgataagct
    14761 tatgcatctt gtaagcgaaa ccgttttatt cactggctcg gtagctgaag atgcacgttt
    14821 tgccggctca tctgcttatg ttgcatcgaa aaatgcattg catgcttatg cggcaggcat
    14881 atccacaaaa attcaaaata caggcgccag atgtatttac tatatgccgg ggattattga
    14941 tggtggaatg ggaaatttac tgaacagaga acaaaaagct gcaggaatga tgcaaattga
    15001 acaaaatgaa cctttagaaa ccggccgcat agctgaaaga atggttaagt cgctatacct
    15061 gcccaaagtt accaataccc gtgacagctt cgagaataat ttgctggtac gaaaagacgg
    15121 gtacttcaag tattagttaa attatggtaa tttaataaat tgaattattg attattaata
    15181 acaaaatatt actactaaaa ttagctgatc ttttctacaa caagaaagca gaacttgcta
    15241 ctgcaaacag tactgatatt ggcaccaatc ttagcgtaaa cagcgacaat attgaacgag
    15301 gcattgcagc aatgcgagaa tgggcatcgg ggtctgcctc agttgatggc aaaaccaaag
    15361 gagggctagc catttactct tgttacagcg acccttcatt cgttcttctg gggctttgcc
    15421 tgccaccggc aattctggca aacggaaata atactgcaat acacatttat attccaacag
    15481 tgcttcgtag ttatcgcagt atttttaagc agattgttga tgaaagtgga ttatttccaa
    15541 acataacctt cgttggcagc catcgtgatt ttttcgagca atcggttact tcacccaaca
    15601 tacaatcaat cgttgttttt ggcgatgatt ggataaacaa ctatctgggc agtatcaggg
    15661 atcatgaaaa aacactgata tattttggac caggcaacaa cactgctgta atactcgata
    15721 agagcaacct gactcaggcc attcattcta tcacccgcct taccacgata ctctcgggcc
    15781 aggctgcggt ttgttttaaa agatgcattg taagctccaa tgttgatttt aacgatgtac
    15841 tgcagcttct aagcactcaa tttaagggag ttactacggg aactgatgtc agaacatcag
    15901 tggttacccc tattggtttc gaaagtatta tcagcacagt agccgagagg gtaaatgaag
    15961 cagccggaaa cggtgctgag ttaatacact tcaagattaa aactcttgaa gatggagtaa
    16021 tggttacacc ggcaattatt gcaaatccac atccaaattc agatctttgg cagaagtatc
    16081 actttgctcc ggtactgagc attgtgcagt gtaaacccga agaaatcagc agctttatac
    16141 gtcacaacga ctatcagctc gctgtatcag tatttggcac ggacatgaaa gatgaaatta
    16201 ttgcagactt acaggctgat catgcttttg ttttcgacaa ttgtgatttt cttgatatac
    16261 tacgcacaaa tataggctat cgcggacact ggggtggatt taaaaattcg gcattcacct
    16321 attcgaaaga aagcggatac gttaaaaacg gtggtaaatt tgatttgtat tcactgatta
    16381 ctcaggaaaa taaataatgt aatggaatcg tacatatttt caggcatagg gcacgcagaa
    16441 gcccgtttta aaattaccaa caacgaaatt ggcactgcgt tacacaatta tcatctcagt
    16501 ggattcagca acgataaggt tgtaagcagc aggaattatc aggaattcct gaagcgacaa
    16561 ccgggaaaaa cagcattcga ctatttcacc gaagaaaaaa tgggtttctc cgttcggcat
    16621 catgtaactc ctttccctcc caccaacaaa aagatttact ttgccgagtc atcacttgat
    16681 ctggcagtac gcgcagtacg aaatgctctg aacaacagtg gtgtacatcc ttctcaggtg
    16741 gctgcgtggt tcttaagcac tgtttcacct cacgaacagg ctccgggaat ggcagccagc
    16801 atgaagtcgt atctggtgag catcaacaat accactccgg catttacgct aacctcgggt
    16861 tgcgccggct tcaatattaa ccttgagaga gcagttgaat acctaaagtt tcaccctgaa
    16921 tcgacacatg ttgtagttgt tcattccgaa accatgtctt cgtttcttac gaaccgtatc
    16981 aagtttgttc cgtttgtaac cttcggcgat ggtgccgctg cagtagtgtt atcgaaagtt
    17041 gaaactccct taccacaggg tttactgaat atagtcaatt atcacgatgt ttccatggtc
    17101 gattttgtgg gagtcgacaa aagatgggat ctttttatgg atgacaacgt aattaaagac
    17161 agggcaataa tcaacattcc ggcagcagct gaatcagcgc ttcgcaatac aggctggcag
    17221 actaccgaca tcgatttctt tgtgcctcat caaacaggta acgctatttt acttccggca
    17281 attgaaaagc tgggtattcc gcgtagtaaa ctttttacag atgcgcagat gcaatacgga
    17341 aatgtttcgg gggcaaccgt tcccatgagt ttatcgcttt tacaccagca aaacaagctt
    17401 agcccgggca ctaaaatttt atcagcaatg gcaggtgttg gcggcaacta tggtgccttt
    17461 acctatttag ttcccgagtt ttccgtttcg taccccaata aaatcattca tgaaatggaa
    17521 ggcaaaacag ctatggtact gggagcacaa tctggccttg ggagagcaat cgctgaaaaa
    17581 ctcattgaat atggagcccg tgtatttgct gtaataacag gtaatggagc actcccccac
    17641 atacagcaca atcatctgca gtacattttt tgtgattttt ccagcgacaa agatgtaaat
    17701 tcgttggcaa tacaattatc agcctctgaa atagctattg attatcttat tcagacctca
    17761 tcggagttaa cacaaccgga aaataacaac tttttttcac cgttggagat aataaaagcg
    17821 agtgtaccgc aaaagccaca atgtattgtt gcagtaggca acatttcgga gaacgtgggg
    17881 tttcccgata aacacgacta tgtttcatcg ctggctgcct tgcacggatc attggcctcg
    17941 gcttccggtg aatttttgag ttatggtatt cgcctgattt attatatacc cggatttacc
    18001 gaggatgata ctacgctgca aatggatgaa agatcaagat tcaggctaat gatgcaggcg
    18061 ggtcaggaac aagttctcag cataaacgag ctgtcggata gaatagttca atcattgtat
    18121 atacttaagg tggaaaacac atggagctat tacgaaaatg caatggtgtt gcgatgcgac
    18181 ggattcaaac ccatggttga tttataaatt taatttgcaa ctatgtattg cttttcatat
    18241 tataccgtac cgcagaaaaa ttatcgggag taatactgtg tttttagaga taatattctg
    18301 aacaatcacg tgaattaaat gttataacaa taaactttta aaacttaaac aatggaaaaa
    18361 actgcaatat ttcaataccc tgtgattgat atcatacaaa aaagatggag ccctgtacta
    18421 ttcgatggta aaactatcag tgagcaacag gcaaatactc tggtagaagc ggcaatgtgg
    18481 gcacccagtg cttacaatga gcaaccctgg cggtatatag ttgcacataa gggaagcgag
    18541 aaattcgata aaatactcgc agcactgata gagttcaatc atgcatgggc aaaagaagcc
    18601 tcactgcttg tggtaagcct tgcaaagaaa atctattcgc acgacggtaa accgaactat
    18661 cactatatgt acgataccgg agctgccaat gcgtttatga taaatcaggc tacttcgatg
    18721 ggtattttct cacatagcat gggaggaatt tattacgata aaatgagaga attgtttgcc
    18781 attcccgaag agttagagat ttgctcagta attgctttcg gataccatag cgaagcttcg
    18841 ggtgctaatg aacaactact gcagcgtgaa caacaacccc gcatgcgcaa agccatgaaa
    18901 gatgtagtgc tgcataaata acactgtttt gaacagtact aaaaatgtca agacaacagc
    18961 cgcgggtatg aaacgtaacg gtttccggtt attatactgt ccagttacac cggacttgat
    19021 tcgtgacatt atgttgtgat gttggctgga atactaccgt ataatgtgcg caaggcttga
    19081 atgatatacc cgggcgtgtt gtttctcgga catcatgttt tacaggaaag acattcgcca
    19141 caataccaga tagtatcgct tttcaataag cgttcaatgt cagtttcatt tgctttctga
    19201 actgtatcaa caataatccg gggatcatag tcataaaact atgcagcggg acaaattgct
    19261 gtgcatgtgt accacattta gcaggcattt aatccttcca tgatgcgaat atctttattt
    19321 aaaaattcaa ataatatttt catttttttt aggaatcata acccaattta tagctttctc
    19381 attgcaaaca gagatacatt tgaggcataa atcacacttt tcctggcata ttacatattt
    19441 ataagggtta catgggactt catacgaaat gcaatattta atgcatttac tacaattttg
    19501 attttgtatt tttccaattg catttttggg gcaaacctgc acacatttac cacacattac
    19561 acatttccaa ttatctatac taagtatttg ttttgctaat gtcatgacga aagttatcta
    19621 tttattagta attatgttat aagcagattt tattcctgtt gcaaaatccg atttcggagc
    19681 atattccaca acgcttttac aattaaccat cgcttcaacc acatggggat taaaaagaag
    19741 ttttccgaca atactaatat tgatactatt gcaataatcc tctatccggt tagtcatttc
    19801 agaattcaag tcatacttat taataattac ccaagttttt aaattaaatt ttgttgtaat
    19861 ttcgatagtt ctttttaaat catgtattcc cgaaatagtt ggctctgtta caattacaac
    19921 atggtcaacg cctgtgattg ttgaaattac agggcaacca atacccggag gaccatcaat
    19981 aattattgtt tcaatatttt ctgctttggc tattgtttta gctttatcac gaacaatatt
    20041 tacaagtttt cctgaatttt cttcacctgg tgcaaggcga ccatatacca ttttcccatt
    20101 gcgaaatgtt cctgcataca tacggctttt attactgtca atcatgctaa ttgccgaggc
    20161 aggacaaata cgtgaacata gtttacagcc atcgcatgag gtgtctgaaa taatcacctt
    20221 atcattcaat aatgaaatag ctccaaaacg acaataatca atgcacaact tgcaattcgt
    20281 acataatgaa tagttaattt ctgctttttg tccgccaata tatacctgta cttcttcttg
    20341 tgtaggattg aaaagaatat acaaattagc ggcatccaca tcacaatcag ccaaaacaac
    20401 tttttcgggt aatgttgcaa aggcagcact tatgctactt tttcctgttc ctcctttccc
    20461 gctaattacg gctatttcca tattcttcaa ctattgagtt atacattgaa atgaagtgct
    20521 gttcccattc cggtttatat ttcacaaata attctccttt tgaataaaaa gatgctattt
    20581 ctttatcgaa gggaatttcc attaataatg gaatttgtat tttttttaaa taatcataaa
    20641 tttcattatc accaagacct gcacggttta taattacacc acaggtttta ttcatggttt
    20701 tcagcgtctc aaccgattgc tttaaatcgc ttaaaccaaa aggcgttggt tcggtaacca
    20761 atataacaaa atcagctccg acaacggtct gaataaaagg gcacgaagta cccggaggag
    20821 aatctaaaag aataatccca ttgttaccag cttctttaat tgcctctttg attatagaaa
    20881 cgggcgaaaa aacgcctact ttcattttgg cttcgataat tgtagatttt tcggtattac
    20941 taaagcaact tacggtacca agcgaaacat ctttttcgct gattgcatca agtttacagg
    21001 ctactgtgca agctccgcaa ccgtgacata aatcttcaat cactttaatt atttttgccg
    21061 gaggtaaaat aaaaattgcg ttgtaattgc agtattcgtg acattttccg cagaaagtac
    21121 atttgctttc atctattaca ggaacttttt ggtttacctc aaaagatttt tctttctctc
    21181 cgataaaaaa taccaaatcg tttggctctt cggcatcaca atcaaccaat gaaatattga
    21241 tattatgttg ttgaagcaca taaaacagat tggtagaaat taaagttttc cctgttcctc
    21301 cttttccact ggcaatagca attttcattt tttcaatttt aatcatattc caatcgagtt
    21361 tgctcatctg caatagtggg cattatagcc tcttgccaaa acttactttg ccatgtttta
    21421 agtttattat gtggtaaaaa atatttttgc ggaattgggt gggttccgat aacaactttt
    21481 aaattatatt tatcttcgat aaattttttg aaataatcaa ttcttgggca aggcggatag
    21541 ccaactacca tacctgttgc aaaatgaatt acggttacgc cgtttttttt catttcttcg
    21601 ggtgcatact caatattgcc acccggacaa ccgccacagg tagtatatgc agcaatttct
    21661 acttctttat ccttgtattc ataaaaagca ccttctctgt tttgcaatgc tctgaaacat
    21721 ttgccaccgg cacaagtatg gtaacgattg cagatgataa ttccgatttt tattttttgc
    21781 ataacaatta ttcactatga ttataaacaa taaaactacc tttttcaaca tttagtttct
    21841 cggcgatcac atcgcatttt accatcatca gaaagaaaat tcttgagtta ttttcattca
    21901 tcaatccttc ataattaccc tgcccttttg aaattattaa atcagccgaa ttataaacat
    21961 caataaactc tttgctgcac ttatgcaata cggtagatgg agcattatat ccgtttgaaa
    22021 taacatcggc aaccagattc ataccaacat attcagcatc ttcaatagta acatcattaa
    22081 gtaccggata ttcttttaca gcatagatga tgtcgttatg catcatggtt tcaatgaaaa
    22141 gcctgtcaaa aacaatttca cccgcattat cgcccaagta taaaattctt tttgcctgtt
    22201 taattaattt ttgaagctgc ttagagtggt caatggcaaa agaagatgta aatacagttt
    22261 caatcgtttc gtgaatatca aaactgttat ttgctccata atccataata ttggctgcaa
    22321 ttgcaagctt taatgccaaa acaaaaggat tttctgatac aagaactttt ggtttccatt
    22381 cagcatacaa ccctaatgct atatcgttac ttaatttctt ttcttcagaa taagggtcag
    22441 atactttaat taaattgcaa aatttattat gtaataaagg ttgaaactca ggactagaga
    22501 gatgattact tgtacttatt ttttctttaa aaaaagctat aaactcctgt tgtaggtatt
    22561 cgttaacatt aaatttttcg aacagtcgtt tatagctttt cagaaagcat ttttcacatc
    22621 ttacatccac aatatagctc ggtttaatgt tgacagttcc cgtgttcatg attttcatca
    22681 tgattgcaat agtttgcact tagacttaac ttgttttcca aaaaatcggt tacaagttct
    22741 ttggctgatt ttacaggtgc tccaacaaaa gcattaatat tttgctgatt gaaaagcatg
    22801 attgcttgtt gccccatacc tccggcaata acatcagtta cgccaaattg agcaatccat
    22861 ttcggatata agccgggcac atgttccggc ggaactgctt ctttaacttc tgttatttta
    22921 ttgttttcaa catttactag agcgaatgtc tggcaatgcc cgaaatgagc acataatact
    22981 ccgttttcta acggaatagc aataattttt ttcatgctct tattttttta gaattttctt
    23041 taaaacagtg tctttatcta tctttttagt acagaaaaac cagtcatagc actttgtgtg
    23101 ttcatcttgg ctttccattc tttcttttgc aactccacaa gaaccagctg ttggtacaat
    23161 tacttcatcg cctggtctcc agtctgctgg cgtagctaca ctaaactcat ctgctgtctg
    23221 caatgcaaga acaacacggt acaattcatc aaaattacga cccaaactaa gtgggtaata
    23281 aataattgtc ctgataatgc ctttcggatc aattacaaaa actgcacgaa cagctttagt
    23341 gttgctttct ccaggcatca tcattccgta tttttgagct acttccattg ttatgtcctc
    23401 aatcaaagga aatgttactt caacattttt cattcctttg tattcaattt tttctttgat
    23461 ggttctcaac catgcaatat gactgtaaag cccgtcaaca gaaagtccaa cgagtttaca
    23521 atttgcttca ttaaatttag gttctaatgt tgcaaaagtc ataaactctg aggtacaaac
    23581 aggcgtaaag tcagcaggat gactaaaaag aattacccaa ctacctttgt aatccgaagg
    23641 gaaattgatt tgtccttgag ttgtaactgc tttaaattcg ggtgcatttt cgcctatacg
    23701 aggcattgca tttttttctt gattttccat ataatttatt tttaattttt aaactgtgtt
    23761 ctgaaaaata gcgagaggat ttttttatta tcccctcatt attaatacta ctgtccgctt
    23821 ctggaacgat tttgtcgtcc cataccacga cctctgccac ggccaaaacc ttttgcatag
    23881 ccaaaacctc gtccctgaac atttttagga ttgttttcgt ttggattttc attgacaatt
    23941 gtagcagatt gattttttaa actcgcaccg aaattggtgc aacgtcccat ccgacgacct
    24001 gtcataggtc cttgtccatt gggaccggtt tgattgaaac ctggcataat aaactccttt
    24061 ttaaaattta gtaataccaa ttaaattgtt tttaagcgat accctttctt ttaatactca
    24121 ctaattttta tgatgcaaca tttcaagaat atctttaatt tttttatcag gctcttttag
    24181 aactatcatt tgaattttta agctgtctaa cagcggtttt attttcattc caaattcacc
    24241 ggatacaatt ttatttacgt tgcgagaggc aactaattgc actgatgcag ggcctgcgcc
    24301 ttcttctgca tctttatttg gattgggaat aaattccgtt gatttgcttt cagtgtcgta
    24361 aaccacaaag taagcacaac gaccaaatcg ctggtcaatt gttgattcta atgaatttcc
    24421 tgaagatgta atggcaactt tcatattatt gatttttaaa atttctgcaa tttggacttc
    24481 cttttctttt cataaaactt ttacaatgtg gacaaatttg tttgctacat ggctgtccgt
    24541 attgatgttc ttgttcaaac ccgcattcag ggcatataca catatcataa catggcttat
    24601 cagcaatatc ttcatgggta ttctcataat caaacttatc aactcgttga cttccacaaa
    24661 gcggacaatt attaacagat ttttcttttt caggattatt gaagtaacag ccacattttt
    24721 tacaatcata ccagtcgctg tcgaaataaa ctttacctcc ttcaatagaa atttgcctac
    24781 cttcaacaaa tgctttagca attttttgaa gtgcagaagc ataaatccgt gtaaaggtgg
    24841 gtctggaaac acccatcata acagatgcct gatgatgatt aagtatgtca tagtcgctta
    24901 gacgtatggc ttcatattcc tcatataata aattaaccgg ttccgttttt tgtttttctg
    24961 attctggacc ataaggctta aaacccttaa tcactggagg atttgaaact tttcttagtg
    25021 cttttattct tggagacata tttataataa accatagttc gatgcaaaga taatgaacta
    25081 ttgctcattt cgcaaattta tttttatatt tgcagaaaaa tatttttatg gatgacagga
    25141 taatgaaatc agaaataaga caatttaaag tcattttccc aaatacttta aatgaccacg
    25201 atactttatt tggaggaatt gcaatgcaat ggatggacga agtggcttat attactgcaa
    25261 taagattttc aaaaaagaaa gtggttactg tctcggctga gaaagttcaa tttttattac
    25321 caatgaaatc aggtacaatt gctgaaatta ttggaaaagt aacaaaagta aaaaatgtta
    25381 agattgaaat tcaggttgaa atatacattg atgatatgta ttctgataat agacaaaaag
    25441 ctgttgatgc tttatttaca tttgcagcaa taaatgatga aaacaaacct attcctataa
    25501 atactaaaga gtattcaagt caagaggttt agagctttaa aacttgcatt gaattttctt
    25561 ctgctttttt tcataatact ggtaaattta attgttttta ttca
//
LOCUS       DAOOQP010000030        24815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2424_length_24815_cov_5.095638, whole genome shotgun sequence.
ACCESSION   DAOOQP010000030 DAOOQP010000000
VERSION     DAOOQP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_24815_cov_5.095638"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLK75_04990"
     CDS             <1..540
                     /locus_tag="PLK75_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38637.1"
                     /translation="LKTTPDSGQSSPYSDMIISCNGVDFVTMGENIINKIPDFYTYEN
                     KTITHTEEGLETETRTLTISDMGIAIIRANLSTDEKITEITVFTTRANTDKGIGPGSK
                     LDEFIQAYPDYKIWYNGVSAHFVIESPQCNCLQFQIDKQFYNSDMNKLRSGGKITYLK
                     RNEFAAETVISSVRILKTN"
     gene            complement(547..888)
                     /locus_tag="PLK75_04995"
     CDS             complement(547..888)
                     /locus_tag="PLK75_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38638.1"
                     /translation="MTQFETNWTYSEFLAYVLFYAANADLKIKPEERELIIEKVDTAT
                     YNCVKKEFSKDNDFVQLEKIRHYCKNELNTSEAKEKLFRDIEEMFWVDGKYSSIEKNF
                     FTGLKRIILHP"
     gene            complement(942..2486)
                     /locus_tag="PLK75_05000"
     CDS             complement(942..2486)
                     /locus_tag="PLK75_05000"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOY38639.1"
                     /translation="MIKNAAGRMVPDSINGQPAIPFKGVGKHRPQGVKHAPSIPSCID
                     YPEDGNKVVKSLKEALDLCGFRDGMTISTHHHLRDGDMVSNLIFDIAHDKGIKDLVWL
                     PSASFPVNDPLVNYLIDGTINRIEGSMNGPLGRAVSEGKMKGMAVLRSHGGRVQAIQD
                     GEVKIDIAVIAAPSADMFGNATATEGPSACGVLGYAKADYMYAEKVIIVTDNLVEVPC
                     FPMLVEGNYVDYVVVVDKIGMPEKIVSGTTTITRSPDRLLIAELTAKFCEITGMLRDG
                     CYMQAGAGGTSLAIGYYMHEILKAKGQHCQLGFGGTTKYMVQMLEEGTMKHILDAQAF
                     DLDAVASISRNKNHHHYPVFNAYNFHSKGNLTSMMDIMILGATEVDVNFNGNVVTHSD
                     GYLLHGIGGWQNCLHARNVILPVPLFRDRIPVIVDEVTTLVGPGEMIDVIITERGIAI
                     NPKRLDLIEAVKNSGLPIVTIQQLKAECDAMCGIPKKPKYKDNVVAAIKWVDGTVIDC
                     VRQLDK"
     gene            complement(2488..3708)
                     /locus_tag="PLK75_05005"
     CDS             complement(2488..3708)
                     /locus_tag="PLK75_05005"
                     /inference="COORDINATES: protein motif:HMM:NF026954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOY38640.1"
                     /translation="MSLRIAEAGNEGPKVRSDCKISIELKESGGLEINLKSKVQSLYG
                     DDINNQMIALSQFFGITNATVTIIDQGALPFVIAARFEAAWKQLANDGKEWLPDFLPQ
                     NKYHTRRDLFRFTRLYLPGNTPAMMLNAGLHGSDGIILDLEDSVAPAKKDEARLLIRN
                     VLRCIDLFGSERMVRINQGQRGLDDLKYVIPHNVNLILVPKCESVEYLHEVDATIEKY
                     KAPEFKNHPVYLMPIIESALGIEMAFDIARATPNVVALAIGLEDFTADLGTKRTQEGT
                     ESMYARWRLVNACKAAGIQPIDSVYSDVDNMDGLRENVKTSKSLGFEGMGCIHPRQVP
                     VIKDSFKPDDEELGKAMKIVLAAEEATKAGLGVISLGSKMIDPPVVKRAERTLNLALN
                     LKIIDKNWRDNYVS"
     gene            3898..7047
                     /locus_tag="PLK75_05010"
     CDS             3898..7047
                     /locus_tag="PLK75_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOY38641.1"
                     /translation="MKSSLFLLCMLIIPVFLLAQSYEQAQKLFENGQYKDAAQQFDKF
                     CNMYEKNGSGSDTLLYPSALVYRARCYDLMNQYKKAEDAYQIAKIYFEENDIIYNENY
                     ALILNNLALINENIGNYQNADFLYGQALLIRKKVMGENHSQTIETMLNIANFYKNNGR
                     YVESEKYFNEVLAATVNVHGSESIEYALALHGFASMYIALGNGSKAEQMLTRAAEISF
                     SDTTSNNDVYLMIENDLGVIYFNRSEFEKAEDAFTMVCSVKKLQPNFRDADYATYLQN
                     LASVKIVLGNYENAKVLIDEALSLITQVYGNRSLNYASVLYTSAALSREMRDYKAAEA
                     ALKETSKILRKSSGSKSLFYAKSMSGLATVYYDMSKIAQNEEEKKSLLSQSESIYADV
                     LSIYESSLGITSLSYISTLSSIATVFQQQHDFVKTEEYLTKAIELLQSTGNDKTNEAF
                     IPFSNLAALYYEEGKTEKCDKAFTDCNALFNILLENNFRFLSEREKLLYIESVRYNIE
                     VYKSFLLKRYESNPEAGCNFYNLELRTKGMVLNSGIEIRKSIVNMGDSLLLATYDQWL
                     TLRSELAMQLSLPESQRMPGWDSIENLADNFEKQWVKGCPYDLALAKTEWKSVQQTLQ
                     PDEATIEFSRFQYCSPKGWSDSVVYVALIIRSDVTYPVVVYLCKEADVDSLVMSQGNT
                     DAGFASRLYRGMVLPNLGNEKNDVKILYELLWEPMEKYLEGVEKIYYSPSGLLHQISF
                     AAIPVTDSILLSDKYILIRVSSTSVLSDSLQRGFSNPHSILAFGGISYDTDAETQLAV
                     ASKYYDENYPSGDNTYALSEAETASRGGNWEYLPGTLNEVQGIHLLARKYNLISTPVT
                     GNEAVEEVIKNISAVGTPDIIHIATHGFFFPNQGNSDLQQFFTTGEKQQAFRKSQNPL
                     QRAGLLFAGANSSWKGRKMPPPVDDGILTAYEISNLYLPKTRLVVLSACETGVGDIKG
                     DEGVFGLQRAFKTAGVDYIVMSLWKVPDFETSEFMQLFYAKLFGGLPIEEAFTQAQNA
                     MRDKYRSEPYKWAAFVLLH"
     gene            complement(7061..7693)
                     /locus_tag="PLK75_05015"
     CDS             complement(7061..7693)
                     /locus_tag="PLK75_05015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY38642.1"
                     /translation="MVPSNLLKLYKVFGRQPFALLDAGSGNFSASKIKRHMPQCNYYG
                     IDITRDYNYRPDDFELMQGFWLKDLTQLQFDDIPDNTFDALLMTHIIEHLHNGDQVIQ
                     GLLKKLKKGGYVYIEYPSEKSVNFPSKKGTLNFYDDPTHVRLYSLHEVKSIVSKCNFE
                     IIDSGTRKSFRNIAMMPIKMVHNKIKYGYVMASVYWDWYGFAEFVWAQKK"
     gene            complement(7760..8080)
                     /locus_tag="PLK75_05020"
     CDS             complement(7760..8080)
                     /locus_tag="PLK75_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOY38643.1"
                     /translation="MKIGIIIETNEPEKAWNAFRFAVTARKQKHEVKVFLMGEAVECE
                     GLIHATYNVDEQLNAFVALGGEILACGTCLKSRNLGSTDSCPISTMVDCLNVVLWADK
                     TVTF"
     gene            complement(8160..8573)
                     /locus_tag="PLK75_05025"
     CDS             complement(8160..8573)
                     /locus_tag="PLK75_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOY38644.1"
                     /translation="MKAKIVLFVIGTFFLITQLHAQCGQVHEKTPTSIGIILYSNDVE
                     TLWNALRFANFAKSSGDTVSIFLLGKGVELDILVKSNADLKDQTETFLGSGGTILGCG
                     TCLNSRKMNEPQVCQFSSMSDLYDIVRKNKIVLTF"
     gene            complement(8684..9103)
                     /locus_tag="PLK75_05030"
     CDS             complement(8684..9103)
                     /locus_tag="PLK75_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38645.1"
                     /translation="MLRNVFIGQLIKTKIAILFAGLFIVFQSCSILPKIDHTRMDARK
                     TVRNCYRLVEEYTTLGAAAAEDSVITQTEADALNSIEINIDSIANSFYATHENRQVLD
                     DMHDKMSRKPKYSKKGSKLLEASMLLFDCEGFELLLE"
     gene            complement(9126..10298)
                     /locus_tag="PLK75_05035"
     CDS             complement(9126..10298)
                     /locus_tag="PLK75_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38646.1"
                     /translation="MTIIITICALLLLAYAFDLTSSKTKIPSVILLLFLGWVVRQITI
                     LLNFRLPDFTLILPILGTIGLILIVLEGSLDLELNKSKLKLVKKSLLGSLLSMLAMAF
                     SLGYLFKYFGDVSLIDGLTNAIPFCVISSAIAIPSVRHLSSSLKEYIVYESSFSDISG
                     VVFFNFVALNDNFNASSYGHFALQLLIILIISFVATYVLTILLGRIEHHIKFIPIILL
                     IILIYAITKEYHLPALIFIMLFGLFLGNLDKLKRFKFINSNRLDNLGKEVIRFKEINV
                     EITFLIRALFFLLFGYLIETPEIINTQTLIWAIGIVMLIFAFRTVQLLISRLPLIPLL
                     FVAPRGLITILLFLSVAETQQIPLVNKSLIIQVIILTALIMMVGIIITKKGAEIET"
     gene            complement(10483..10848)
                     /locus_tag="PLK75_05040"
     CDS             complement(10483..10848)
                     /locus_tag="PLK75_05040"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOY38647.1"
                     /translation="MKTIRHKATETMLVFTAVALLVYLLFNDNALWLYIALGMAFTGI
                     FIPRFAEIVHIAWMFFSRKIGLVVSTVILILIYFFVLVPLAAISRLFGRQIISKSNRQ
                     SLWQSGKVNYGPSHFRDLW"
     gene            complement(10850..10999)
                     /locus_tag="PLK75_05045"
     CDS             complement(10850..10999)
                     /locus_tag="PLK75_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOY38648.1"
                     /translation="MEFLKDLFRFLGERKKYWLIPIIIVLALVGILLIFGGSSSIAPF
                     IYTLF"
     gene            complement(11001..12860)
                     /locus_tag="PLK75_05050"
     CDS             complement(11001..12860)
                     /locus_tag="PLK75_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOY38649.1"
                     /translation="MKILGISAYYHDSAACITIDGKVVAAAQEERFTRKKHDNSFPAQ
                     AVGFCLKFCNLNIDDLDAVVYYEKPFLKFERLLETYYAYSPRGVVSFVKAIPVWTKEK
                     LFMKKLLRDELREIMPYKKRKLQLLFTSHHLSHAASAFYPSPFNEAAIVTVDGAGEWA
                     TASLCHGREKSISLLKELRFPHSVGLLYSAFTYFLGFVVNSGEYKLMGLAPYGNAGDA
                     QTLTFIEKIRSEIVAVNQDGSVYLHQKYFRYATGLRMINLAAWEKLFGFRIRHSEDPI
                     AQHHCNLALAIQEVTEDIILRMAAEAKKCTQSENLVLAGGVALNCVANGKILKSGLFR
                     NLFIQPAAGDAGGALGAALAAEYIFFDQDRVIQNPDSMNGAFLGPEYTDTQVETTLKK
                     YYANYRHFESFDILAGEVASFIHQGKVVGWFQGRMEFGPRALGNRSILADARNTAMQK
                     QLNLKIKFREGFRPFAPAVIAEDSNAYFHIQCASPYMLLVADINQSLRQPLPDNYQNL
                     PTMDKLYTQRSSVQAITHCDFSARIQTVHAETNPRFYRLLQEFKSITNCSVMVNTSFN
                     VRGEPIVCTPEEAFKCFMSTEMDVLVLNNFVLIKTDQQEVLKNYTFVSSYKPD"
     gene            complement(12870..13160)
                     /locus_tag="PLK75_05055"
     CDS             complement(12870..13160)
                     /locus_tag="PLK75_05055"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOY38650.1"
                     /translation="MKRVSTTSISDLIKQYISDNNLEYGIVSARLVNSWETAVGKMIA
                     NSTKDLYVHKRKLYVTLSSSIVRHELMMIKSGLISRLNELAQANVIDDIVFR"
     gene            13330..13833
                     /locus_tag="PLK75_05060"
     CDS             13330..13833
                     /locus_tag="PLK75_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38651.1"
                     /translation="MELSLKQIIANLSATEETTVLDSVNYLAGNGKPESVQPLIECYF
                     ITFSQPVKKRILELFCDIKDSRAAAPWVETMMKHTQSDGFRELLSCCWQSSIGFQHHL
                     ESFADIVATADLYTSIEAISVIESNIANAGHNEIMQMASQLRDSIPAMSPEKQRLIDS
                     FLAGYEL"
     gene            13850..14557
                     /locus_tag="PLK75_05065"
     CDS             13850..14557
                     /locus_tag="PLK75_05065"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOY38652.1"
                     /translation="MPERVFRCKQFSVIHRNSAMKVGTDAILLGAWASIPESGKILDI
                     GSGTGIISLMMAQRSVCDITAIEIDADAYEESLVNFGNCPWTERISVYNCNILKFSSP
                     KKFELIISNPPFFDNRCFDSGNKRNLARNNESLSLSSLIGIVAKLLTKTGCFCMIYPH
                     DQLKQLLEFTTLNNLYINKLTYVIPRFGMPPVRVLVKCSFQEMFSEESEIIIEGCKRG
                     VYTEEYIMLTKDFYLKF"
     gene            14774..15856
                     /locus_tag="PLK75_05070"
     CDS             14774..15856
                     /locus_tag="PLK75_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HOY38653.1"
                     /translation="MNNKKTVRPVKNQEPRKKNKSNGRSLVVTIIAFVAGGGLGLILG
                     FFISGKEEKIIETQSDQQQQFTEIRAGGYEFTNPLLDCENFHPSVSRNHVSLKTQLND
                     YVNSALQAGLAESISVYYRSLNNGPWIGINEDSFYMPASMLKVPLMIAVYKKAQYESG
                     FLNKKIDYTRVLDPSFTQNIVDNVKIEVGKSYTVEELVEYMIMHSDNNAKELLLAQIG
                     DEFLINVMIDLGVNIKNRDLSVDFVSPKEYSAFYRILYNSSYLSRDYSEKALKLLSKT
                     MFSQGIPGKLPKTIKIAHKFGERGFQSTDIKQLHDCGIVYFENNPYLICIMTRGNSFD
                     NLVGIISDISEIVYKSVNEDISSQGK"
     gene            complement(15976..17766)
                     /locus_tag="PLK75_05075"
     CDS             complement(15976..17766)
                     /locus_tag="PLK75_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOY38654.1"
                     /translation="MIMFRERFNSILKKFLIWRLHKVPDRTFIMFIAVLTGIAAGLAA
                     VLLKNSVHYIRMGVTTWFSPDNENYLFIISPALGIFLVIIFLRQILKKKLEPGVAMVL
                     RSKSQKNGRIEGHNMFSHLISSALTIGFGGSVGLEGPTVATGSAIGSNIGRLFHLNSK
                     QIVLLIGCACTAAIAAIFKAPIAGLVFALEVIMLDLTMSSLIPLLLASVAATLTSYLF
                     LGHDVIYFVERIDSFWMRHIPYYILLGILTGFLSLYFTKVYMFLHNGFAKIKSWITRF
                     FIGSFALGLLIFLFPGLYGEGYEVINASLHGDYSYLFNNSLFYAFSDSIWIVIMVFVA
                     IILLKVFAAGVTFGAGGVGGIFAPSLFIGANVGLFLSYVLKLLGFNVSSTNFALVGMA
                     GTIAGVIHAPLTAIFLIAELTGGYGLFVPLMIVATLSYLTVRLFNQKSLFLQQLSKTG
                     EIITHHKDKTVLSMMKVDQLIESNFICVKSDNTLRELIFAIERSTRNIYPVVDDEGKL
                     EGIVFLDHIRHIIFKQELYDTTYVSNFMFMPQPLVNIKDTVEAVAEKFQDSGNYNLPV
                     VDDDNKYVGFISRAKVFSNYRRLTKQISDE"
     gene            complement(17925..18680)
                     /locus_tag="PLK75_05080"
     CDS             complement(17925..18680)
                     /locus_tag="PLK75_05080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOY38655.1"
                     /translation="MINLILIAFAPVILILFYVYYRDKYEKEPIGKLLLSVLFGALAV
                     IPILITETILSFPASWMDGYLLAGWDAFVVAAFSEESFKFLVFIIFIWRSKHFNEHFD
                     GIVYAVYISLGFALVENILYVVEGGASTALVRAISAVPAHAIFGVTMGYYLGRAKFDP
                     AHRRRLILTAIFLPILLHGLYDFILMSGMPYFLIFFAVYLFVLYRLALKRMKQLSVLS
                     GYRMNKVDEFNNNNSATGNTQETTETDESNPVS"
     gene            complement(18687..19931)
                     /locus_tag="PLK75_05085"
     CDS             complement(18687..19931)
                     /locus_tag="PLK75_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY38656.1"
                     /translation="MKQELFIARKMLKGQKHGASFSRPVVNIAIAGIAIGLAVMLLSV
                     AIVTGFKNEISKKVIGFGSHIRVINYDTNTSYETKPIGLHQSWFNQVRSIKGVRHIQP
                     FVTKAGIFKTDYYLQGVVIKGVDKSFDFSFFENYMVEGSIPAICDTGKTNEILVSKYL
                     ADILKLKVGDKVSSYFIQDPPRMRKFVVSGIYDTQLQDLDKLFIIADARHIQKLNDWN
                     EKQVTGFEILIDDLKNIDLISSEVKSVVSNIPDENGNFLKVSSIKDDYPEIFNWLGLL
                     DMNVWVILILMVSVAGFNMVSGLLVIILERVNMIGILKSLGSTNVSIRRVFLIYAGML
                     IGRGMLWGNVLGIGLCLLQWAFKIVSLDPVSYYVSYVPININFLHIVLLNAGSFVITV
                     LMMLVPSFLVTRVSPSEAIRFN"
     gene            20091..20933
                     /locus_tag="PLK75_05090"
     CDS             20091..20933
                     /locus_tag="PLK75_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOY38657.1"
                     /translation="MDTVSKLQNIATQVRRDVLRMVHSKNSGHPGGALGCADLLTALY
                     FEVLHQKEVFDMAGNNEDMFFLSNGHLSAVWYSVLSRKGYFPVKELSTFRAIDSRLQG
                     HPATAEHIPGVRVASGSLGQGLSVAAGAALGKKMSGDKNIVYCLCGDGELQEGQIWEA
                     AMFATARCIDNLIAIVDYNGKQIDGPVDSVISLGNLKAKWEAFGWIALETNGNDMRLL
                     LNTLNEAKHNTGNAKPVVILMKTIMGMGVDFMMDDHRWHGSAPDNKQLELALQQLPVT
                     LGDY"
     gene            20944..22323
                     /locus_tag="PLK75_05095"
     CDS             20944..22323
                     /locus_tag="PLK75_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY38658.1"
                     /translation="MKKIVFILITLLICTANLTVKAQNNPPLTRVLFILDGSSSMLEV
                     WDRGIKFYTAQQLLTELLDSLSGLPNQDNIQYALRVFGHQSAAPPEDCNDTKLEVPFG
                     YNNSEAIIAKIESIKPKGTTPIARSLLDCANDFPDCDNCRNIVILITDGMERCGGDPC
                     EVSIALQKQGIILKPYIIGIRLDTQIADYLSCIGNYFDVGNADEFADALNTVISEVTN
                     LTSVQINLLDIYGNPTETNVALSFHDNLSGNIRYTFMHTMNDKGQPDTLHVDIMSVYD
                     LKVHTIPPVYLDTLIVQPKKLNVFEVPAPQGSLKVVVDGNDPDDNDVKCIVRQTGLME
                     TIHVLDAEKEQYYIVGLYNLEVLTLPRIYIDAVEVKQDVQKTLRIAQPGRVIINFKSP
                     VYGSLLFENGDVLTNIYDFIPDRVVYNLRLQPGYYRIIFRDKNQRKTEKSLEQKVRVK
                     SGETYIFNL"
     gene            22329..23288
                     /locus_tag="PLK75_05100"
     CDS             22329..23288
                     /locus_tag="PLK75_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOY38659.1"
                     /translation="MTTYTYTETKDTRSGFGAALSQLGKTNPSVVALCADLTGSLKMD
                     AFAKAHPERFFQVGIAEANMIGVAAGLSITKKIPFAGTFANFATGRVYDQIRQVVAYS
                     NKNVKIAASHAGLTLGEDGATHQILEDIGLMKMLPNMTVINPCDYNQTFAATIASANH
                     EGPVYLRFGRPKVPNFTPAAQEFVIGKAILLNEGKDVSIFATGHLVWEALQACEMLES
                     RGISAEIINIHTIKPLDTVAVLKSVSKTGAVVSAEEHMKNGGLGDSIAQLLAENYPLP
                     QQFVAVDDTFGESGEPMSLMKKYGLSSDAIVNAAIKAIGRKCK"
     gene            complement(23297..24286)
                     /locus_tag="PLK75_05105"
     CDS             complement(23297..24286)
                     /locus_tag="PLK75_05105"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOY38660.1"
                     /translation="MFRLLTCIILLCNAGIAISQCDTVKVEALKGEGVYALLKRNHYE
                     ASYFSDFVRINKKYLGPENTLIAGKIYALPCKPDSIIVPVVSDSVPGIVCIDSVLSGT
                     VFYLIPGHGGPDPGAVATVDGNMITEDEYAYDIALRLKREIESHAGVAIMIVIDPNDS
                     IRNEKFLVHDTDEICYPDKSIPRDHNERLLQRVYAVNNLYDSYGALVHQRVIEIHLDS
                     RPESQKIDVFFYYHTTSTKGQRLAEIMLSTFEAKYKEHQPNRVYNGSVSPRSLMVLRK
                     TKPVGVFMELGNMSNVNDQKRFLDPDNRDILAKWICAGLIEEYNEVGKKQSLN"
     gene            24591..>24815
                     /locus_tag="PLK75_05110"
     CDS             24591..>24815
                     /locus_tag="PLK75_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38661.1"
                     /translation="MKKIGVTIMAIFIVGSLAMAQTVNKGGQHNNTQNGKSVEKASVA
                     PTQKLNNHSAEFRGSTPDAVRPVDQAQQKAF"
BASE COUNT         7640 a         4953 c         4966 g         7256 t
ORIGIN      
        1 ctgaaaacaa cacctgattc aggacagtca agtccttaca gcgacatgat tataagttgc
       61 aacggtgttg attttgtaac tatgggcgaa aatattatca ataaaatccc cgatttttat
      121 acatatgaga ataaaaccat cacccatacc gaagaaggct tggaaaccga aactcggaca
      181 cttaccataa gcgatatggg tattgctata attagggcta atttatcgac ggatgagaaa
      241 ataacagaga taacagtgtt cactacgcgt gcaaacaccg ataagggaat tgggccgggt
      301 agtaagctgg atgagtttat tcaggcatat cccgactata agatttggta caacggtgtg
      361 agtgcccatt ttgtaataga atcgccacag tgtaattgtc tccaatttca aattgataag
      421 cagttttaca attccgatat gaacaaactt cgttcaggag gtaaaattac ttatcttaaa
      481 agaaacgaat ttgcagcaga aactgtaata agctctgtaa ggatacttaa aactaattag
      541 ctactcttat gggtgaagga ttatccgctt taaaccggta aaaaagttct tctctattga
      601 tgaatattta ccatctaccc aaaacatttc ttcgatatct ctgaacagtt tttctttggc
      661 ttcgcttgtg tttaactcgt ttttacaata atgtctgatt ttttcgagtt gtacaaaatc
      721 gttatctttc gaaaactcct ttttcacgca attataggtt gctgtatcca ctttctctat
      781 aatcagttct ctttcttccg gttttatttt caaatcggca ttggcagcat aaaatagtac
      841 ataagccaaa aattcgctgt atgtccagtt ggtttcgaat tgtgtcatga tgtgatttgg
      901 ttttaagcgg ttaataattt tatttaaaga gtttcgtgtt gctatttatc cagctggcga
      961 acacagtcaa ttacagttcc atcaacccat ttgattgctg caacaacatt gtctttgtat
     1021 ttgggcttct tgggaatgcc acacatagca tcgcattcag ctttaagctg ttgtatcgtt
     1081 acaatgggta agcctgaatt ctttactgcc tcaatcagat ccagacgttt cggattaatt
     1141 gcaatacctc tttcagtaat aataacatca atcatttctc cgggtccaac taaagtagta
     1201 acctcgtcga cgatcacagg tattctgtcg cggaacaaag gaactgggag aataacgtta
     1261 cgggcatgca ggcaattttg ccatccacct attccgtgaa gaagataccc atcggaatgc
     1321 gtaacaacat taccgttgaa atttacatcc acttcggtag caccgagaat cataatatcc
     1381 atcatcgaag tcagattccc cttggaatgg aaattatagg cattgaaaac agggtagtgg
     1441 tgatggtttt tattgcggct tatagaagca actgcgtcca gatcgaaagc ctgagcatcg
     1501 agaatatgct tcattgtacc ttcttccagc atttgcacca tatattttgt tgttcctcca
     1561 aaaccgagct ggcaatgttg cccctttgct ttcagaattt cgtgcatata gtaaccaata
     1621 gcaagcgaag tgcctcctgc accggcttgc atgtagcacc catcgcgcag cattccggta
     1681 atctcgcaga attttgcagt gagttcagca atgagcaatc ggtcgggact gcgggtgatt
     1741 gtggttgttc ctgaaacaat tttttcgggc ataccaattt tatcgaccac tacaacatag
     1801 tcgacgtaat taccttcaac gagcataggg aagcaaggaa cttccaccaa attatctgta
     1861 acaataatca ctttttcagc gtacatgtaa tcagcttttg cataacctag tacgccgcaa
     1921 gccgacgggc cttcggtagc agttgcattc ccgaacatat cagcactggg agctgcgata
     1981 acagcaatat caattttgac ttccccatcc tgaattgcct gaacacgtcc accatgagag
     2041 cgcagtactg ccataccctt cattttacct tcagacacgg ctctacccag cggaccgttc
     2101 atgcttcctt caatccggtt gattgtgcca tcgatgagat agtttaccaa cggatcgttt
     2161 acgggaaacg atgcagaagg cagccaaaca aggtctttta tgcccttgtc gtgggcaatg
     2221 tcaaaaatca ggttacttac catgtctccg tcgcgaaggt gatggtgagt cgaaatagtc
     2281 atgccatcgc gaaatccaca taaatcaagg gcttccttaa gcgatttcac aactttgttg
     2341 ccatcttcag ggtagtcgat gcaacttgga attgatggtg catgttttac cccctgaggt
     2401 ctgtgttttc cgacaccttt aaacggaatt gcaggttgtc cgttgatact gtcgggaacc
     2461 attcgtcccg cagcattttt tatcatatta gctgacataa ttatccctcc agtttttatc
     2521 aataattttt aaattaagtg caaggttcag ggtgcgttcg gcacgtttca ccaccggtgg
     2581 gtcgatcatt ttcgatccca atgaaattac cccaaggcct gctttcgtgg cttcttcggc
     2641 agccaaaaca attttcatgg cttttcccaa ttcttcatca tcgggtttaa aactatcctt
     2701 tattaccgga acctgccttg gatgaataca tcccatgcct tcgaagccaa ggctttttga
     2761 tgtttttacg ttttcacgca aaccgtccat attgtcaaca tcgctgtaca cagagtcgat
     2821 gggttgtatt ccggctgctt tgcaggcgtt tacaagtcgc catcttgcat acatcgattc
     2881 ggtgccttcc tgtgtgcgct ttgttcctag gtctgctgta aaatcttcga gaccaatggc
     2941 aagggctacc acgttggggg ttgctctggc aatgtcgaaa gccatttcaa ttccaagggc
     3001 gctttcaatt attggcatca gataaactgg gtgattttta aattccgggg ctttatattt
     3061 ttcgattgta gcatccactt cgtggagata ttcaacactt tcgcatttgg gtacaagaat
     3121 caggttgaca ttatgtggaa tcacatattt caggtcatct agaccacgtt gtccctggtt
     3181 gattcgtacc atgcgctcgg atccgaacaa atcgatgcac ctcagtacat ttcgtatcaa
     3241 aagacgggct tcgtctttct ttgcaggagc aacggaatct tctaggtcga gtataattcc
     3301 atcagaacca tgtaagcctg cgttaagcat cattgcaggt gtattaccag gcaaatacag
     3361 tctggtaaag cgaaataaat ccctgcgtgt atgatacttg ttctgaggta aaaaatcagg
     3421 aagccattct tttccatcgt tagccaattg cttccaggca gcttcaaagc gggctgcaat
     3481 aacaaaaggt agcgcaccct gatcaataat agtaactgta gcattggtaa ttccaaaaaa
     3541 ttggcttaat gcaatcattt gattattgat atcgtcgcca tacagcgatt gaaccttgct
     3601 tttaaggttg atttccaacc cgcccgattc tttcagttca atacttattt tacagtcgga
     3661 acgcactttg ggtccttcgt ttccggcttc agcaattcgt aatgacataa tattcagttt
     3721 ttatttacac aaaattaaaa atcattcgca agcagcaata taattttaaa acataagatg
     3781 catttttttt atcagcaggg cagtttttta tcattgtgaa caatgttgca attaataagc
     3841 tgaagatatt gataactgta ttatttttaa tattttagcg atactaaaac cccatgtatg
     3901 aagagtagtc tgtttttatt atgtatgctg attattccgg tatttttgct tgctcaaagt
     3961 tatgaacagg ctcaaaaact ttttgaaaac ggacaatata aggatgctgc tcagcaattc
     4021 gataagtttt gcaatatgta cgaaaaaaat ggttccggca gcgacacgtt gctttaccca
     4081 tcagcattgg tgtaccgggc aagatgctac gatttgatga atcagtacaa aaaagctgag
     4141 gatgcatacc agattgcgaa aatatatttt gaagaaaatg atattattta taatgagaac
     4201 tatgccttaa ttctgaataa tctggcattg ataaacgaaa atattggaaa ttatcagaat
     4261 gcagactttt tatatgggca ggcattgctt atccgaaaaa aagtaatggg tgagaaccat
     4321 tcacaaacca ttgaaaccat gctgaatatt gccaattttt acaaaaataa cggcagatat
     4381 gttgagtcag aaaaatattt taatgaagtg ttggcagcca ctgttaatgt acatggtagc
     4441 gaaagtattg aatatgcgtt ggcactgcat ggttttgctt cgatgtatat tgcattgggt
     4501 aatggttcga aagctgaaca gatgcttaca cgggctgctg aaatttcctt tagcgacacg
     4561 acatcgaata acgatgtgta tctgatgatt gaaaatgatt tgggagtaat atatttcaac
     4621 aggtcggaat tcgaaaaggc tgaagatgct tttaccatgg tttgcagtgt taagaaattg
     4681 caaccaaatt ttcgcgacgc tgattacgca acctatcttc agaatcttgc ttctgtaaaa
     4741 attgtattag gaaactacga aaatgcaaaa gtcttaatcg atgaagcact gtcgctaata
     4801 actcaggttt atggaaacag gagtctcaac tatgcatcgg ttttatatac ctcggcagct
     4861 ttgagcagag aaatgcgcga ttataaggct gctgaagccg cacttaaaga aacttcaaag
     4921 atattgcgta agagcagcgg tagtaaaagc ttattttatg caaaatcgat gagcggactt
     4981 gctacggtgt attatgatat gtcgaaaatt gcacaaaacg aagaagaaaa aaaatcgctg
     5041 ttatcgcagt cagaatcaat ttatgcagat gttcttagca tatacgaaag ttctttggga
     5101 ataaccagtt tgtcatacat cagcacattg tcgagcattg ctacggtttt tcagcagcag
     5161 catgattttg ttaagactga agagtacctc accaaagcaa ttgagttatt acagtctacc
     5221 ggaaacgata aaaccaatga agcgtttatc ccttttagta atctggcggc gttatattat
     5281 gaagaaggaa aaacagaaaa atgtgataag gctttcaccg attgcaatgc tttatttaac
     5341 atactattgg aaaacaactt caggttttta agcgaaagag aaaaactatt atatattgaa
     5401 tcggtccgct acaatattga agtgtataaa agctttcttt taaaacggta tgagagtaac
     5461 cctgaagccg gttgtaattt ttacaatctt gaacttcgta caaaaggaat ggtgcttaat
     5521 tcaggtattg aaattcgcaa aagcattgta aatatggggg actcactatt gttggcaact
     5581 tacgatcaat ggcttacgtt gagatcggaa ctggcaatgc agctttcttt accagaaagt
     5641 cagcgtatgc cgggttggga ttctattgag aaccttgccg ataatttcga aaagcaatgg
     5701 gtaaagggtt gcccttatga cttggctctt gctaaaactg aatggaaaag cgttcagcaa
     5761 actttacaac ccgatgaagc aactatagaa ttttcgcgtt ttcaatattg ctctcctaag
     5821 gggtggagcg actcggtggt ttatgtggca ctgattatac gcagcgatgt aacctatcct
     5881 gttgttgttt atctttgcaa agaagcggat gtcgatagcc ttgtgatgag tcagggaaat
     5941 accgatgcag gttttgcatc ccgtttgtac cgtggaatgg tgctgcccaa tctgggtaat
     6001 gaaaaaaacg atgtaaaaat actctatgag ttgttatggg agccaatgga aaagtacctc
     6061 gaaggcgttg aaaaaatata ttattcccca tccgggctgc tacatcagat atcatttgct
     6121 gcaattcctg ttacagattc gatattgctg tctgataagt acattttaat acgagtaagt
     6181 tcaacatcgg ttttatctga ttcattgcaa cgggggttca gtaatccaca ttcgatactt
     6241 gcgtttggtg gcattagcta tgatactgat gccgaaacgc aattagccgt tgcttcgaaa
     6301 tattacgacg aaaattatcc ttccggtgat aatacttatg ccttatcgga agcggaaacg
     6361 gcatcaagag gggggaactg ggagtatctt ccgggtacgt taaacgaagt tcaggggatt
     6421 cacttgcttg cccgtaaata caaccttata tcaaccccgg taacaggtaa tgaggctgtt
     6481 gaagaagtca ttaaaaatat ttcggccgta ggcactcccg acatcattca tattgctact
     6541 catggcttct tctttcccaa ccagggcaat agcgatttgc aacagttttt tactaccggt
     6601 gaaaagcagc aggcattccg taaaagtcaa aacccgcttc agcgtgcagg actattgttc
     6661 gcaggtgcca atagcagctg gaaaggacgc aaaatgcctc ctccggttga tgatggaatt
     6721 cttactgcct acgaaatctc gaacctgtat ttgccaaaaa ctcgccttgt tgtgctgtcg
     6781 gcgtgtgaaa caggggtagg agatattaag ggtgatgaag gcgtatttgg tttacaacgg
     6841 gcatttaaaa ctgccggagt cgattatatt gttatgagcc tgtggaaagt acccgatttt
     6901 gaaacttccg agttcatgca gctgttttat gcgaagctat ttggaggctt gccgattgaa
     6961 gaagcattta cacaggctca gaatgcaatg cgcgataaat accggagtga gccctataaa
     7021 tgggctgctt ttgtgctatt gcattaaatt atgatatcgg ctatttcttc tgagcccaga
     7081 caaattcagc aaaaccgtac cagtcccaat ataccgatgc cattacatag ccgtatttta
     7141 tcttgttatg taccattttt ataggcatca tggctatgtt tcgaaaactt ttgcgtgtac
     7201 ccgaatcaat aatttcaaag ttgcacttac ttactatact tttaacttcg tgtaaagaat
     7261 acaaccttac atgggttggg tcgtcgtaaa aattcaatgt tcctttttta cttgggaaat
     7321 tgacactttt ttcagaagga tattcaatat aaacatatcc gccttttttt agttttttca
     7381 gcaagccctg aataacctga tctccattat gcaaatgttc aatgatgtgt gtcattagca
     7441 atgcgtcgaa ggtgttatcg gggatatcat cgaattgaag ctgggtgagg tctttcagcc
     7501 agaaaccctg catcagttca aagtcatcgg gacggtaatt gtagtcgcgg gtaatatcaa
     7561 tgccataata attgcattgg ggcatatggc gttttatttt cgaagccgaa aagttacccg
     7621 accctgcatc cagtaatgca aatggttgac gaccaaaaac tttatataat ttcaacagat
     7681 tcgatggaac cataatgcaa aaataagtgg tgcaaaacta atgattatga agggaaaaac
     7741 taagtattaa aaataaattt tagaaagtta ccgttttatc agcccagagg acaacattca
     7801 aacaatctac catggttgat atcgggcaac tgtcggtgct gcctagatta cgcgatttga
     7861 ggcatgtgcc gcaagcaagt atttctcctc ccaaagccac aaatgcattt agctgttcat
     7921 caacattata tgttgcatga attagccctt cgcattccac tgcttcgccc atcaaaaaaa
     7981 cttttacctc atgcttttgc ttgcgggcag ttactgcaaa acggaatgca ttccatgctt
     8041 tttcaggttc atttgtttcg atgattatac caattttcat aacacaagta ttagtgatta
     8101 ataacagcca tatatgttct tataacaaat aacagccatt tataattgat tgtaatatac
     8161 taaaatgtaa gtacaatttt gttttttcgg acaatatcgt acaaatcgga catggatgaa
     8221 aactggcata cctgaggctc attcattttc cgactgttta aacaggtgcc gcaaccaaga
     8281 atcgttccgc cactgcccag aaatgtttcg gtttggtctt tcaggtctgc atttgattta
     8341 accagaatgt cgagctcaac tcctttaccc agtaaaaaaa ttgatactgt gtcgccggag
     8401 ctttttgcaa agtttgcaaa gcgaagcgcg ttccatagag tctccacatc gtttgagtag
     8461 agaattattc caatacttgt tggtgtcttt tcatggactt gcccgcattg ggcatgaagc
     8521 tgagttatca agaagaatgt accgataaca aataaaacga tttttgcttt cattgttgaa
     8581 ctgattttta acaaaaatga tggtttctgc gaatcttgcg taaacacggg gatagggtat
     8641 aatttcaagt tctactgaat atgaaatata ctatattaat tcattattcc aacaacaatt
     8701 caaacccttc acaatcgaaa agtagcatcg aagcttcaag caatttagat ccctttttac
     8761 tatattttgg ttttctgctc attttgtcat gcatatcatc gagcacctgc ctgttttcgt
     8821 gagttgcata aaagctgttt gcgattgaat cgatattaat ctcaatacta ttcaatgcgt
     8881 cagcttctgt ctgagtgatc acagaatctt cagcagcagc tgcccccagg gtagtatact
     8941 cttctaccag tctataacaa ttcctgacag tttttcttgc atccattctg gtatggtcaa
     9001 tcttcggaag aatactgcac gactggaaca caatgaacaa acctgcaaaa agtatcgcta
     9061 ttttagtttt aatcaattgg ccaatgaata catttcgtaa cattttttaa gctaaactct
     9121 cttaatcaag tttcaatttc agctcccttt ttagttatta ttatacccac catcataatc
     9181 aatgctgtta aaatgataac ctgaattatt aatgatttat tgacgagagg tatttgctgt
     9241 gtttctgcaa cagataagaa caacaaaatt gtaataagcc ctctgggagc aacaaataat
     9301 aacgggatca ggggaagcct tgaaattaac aactggacag tgcgaaatgc aaaaattagc
     9361 ataactatcc caattgccca gattaatgtt tgcgtattga taatttcagg agtctcaatc
     9421 aaatagccaa ataacagaaa gaataatgcc ctgattaaaa aagttatttc aacatttatt
     9481 tccttgaatc tgatgacttc tttacccaga ttgtccaatc gattgctgtt gataaactta
     9541 aagcgcttaa gtttgtcgag gttccccaga aaaagcccga aaagcataat gaaaatcaaa
     9601 gcgggtaaat gatactcttt ggtaatagca taaattaata tgattaataa tattattggt
     9661 atgaatttga tgtgatgttc aattcttcct aatagaatag ttagaacata agttgccaca
     9721 aaagaaatta ttaaaataat tagaagttgt aatgcgaaat gcccataaga acttgcatta
     9781 aaattatcat tgagggcaac aaaattaaaa aaaactacac cagaaatatc agaaaaacta
     9841 ctttcataaa ctatatattc ttttaatgac gaagagaggt gtcgaacgct tgggatggcg
     9901 atagcactgc ttattacgca aaaaggaatg gcatttgtta atccatcgat aaggctgaca
     9961 tctccaaagt acttaaaaag gtatcccaat gaaaatgcca ttgcaagcat cgaaagcaga
    10021 gagcccaaca atgatttttt taccagcttt aattttgatt tattgagctc caaatcaagt
    10081 gagccttcta aaactataag tattaagccg atagttccca gtattggtaa aatcagtgta
    10141 aaatcaggta gtcggaaatt caataggatt gttatttgtc gaacgaccca gccaagaaag
    10201 agcaaaagta ttaccgatgg aattttagtt ttcgatgaag taaggtcaaa agcataagca
    10261 agcaatagta aagcacatat agttattatt attgtcatgt ctttttaact aattatattg
    10321 cccttgcaaa cacatttatc caaaatttga tacgttcata aataaatgat tatcaaatgt
    10381 ttaattattt ttaataataa acggtctaat aatatgtttt tttgcaatgc gaatttagtg
    10441 aatttcacca ataattccta cctccttaca aataatttag ctttaccaca aatcgcgaaa
    10501 atggctgggc ccatagttaa ctttacctga ttgccagagc gattgtcggt ttgattttga
    10561 aataatttgt cggccaaaca aacggctgat ggctgccaag ggaaccagca caaaaaaata
    10621 tatcaggatt aggatcacgg tcgaaacaac cagtccgatc tttctcgaga aaaacatcca
    10681 tgcaatatga acgatttctg cgaaccgtgg aataaaaatg ccagtaaaag ccatgccgag
    10741 tgctatgtac agccataatg cattgtcgtt gaaaagcaaa tatacgagca atgccacagc
    10801 agtgaaaacc agcattgttt cggttgcctt atgccgaata gttttcatgt tagaacaagg
    10861 tgtaaataaa tggtgctatg ctgctgctgc ccccgaatat caatagaata cccaccaaag
    10921 caagcacaat gatgatgggt atcagccaat atttttttct ttcacctaga aaacgaaata
    10981 aatctttaag aaattccata tcaatcgggt ttataactcg aaacaaatgt ataatttttt
    11041 aacacctcct gctggtcggt ttttatcaat acgaagttat tcagcaccag cacatccatt
    11101 tcggtgctca taaagcattt gaatgcttct tcgggggtgc aaacaatggg ctcaccccgc
    11161 acattgaagc tggtattaac cattacactg cagtttgtaa tgcttttaaa ttcctgcagt
    11221 aagcggtaaa aacgcggatt ggtttcggca tgtactgtct gaatgcgtgc cgaaaaatcg
    11281 cagtgtgtga ttgcctgaac cgatgaccgc tgtgtgtata gtttgtccat tgtcggtaaa
    11341 ttctggtaat tatcaggcag gggttgcctg aggctttggt ttatatctgc tacaaggagc
    11401 atataaggcg aagcacattg tatatggaag taagcattgc tatcttctgc aattacagca
    11461 ggggcaaagg gacgaaaacc ttctctaaac ttaattttca ggttcagttg cttctgcatt
    11521 gctgtgttac gggcatcggc aagaatgctc cggttgccca aggcacgcgg cccgaattcc
    11581 atgcgcccct gaaaccaacc caccactttg ccctgatgaa tgaatgatgc cacttccccg
    11641 gcaagaatat caaacgattc aaaatgccta tagtttgcat aatatttttt cagggtagtt
    11701 tcaacctgag tatcggtgta ctcaggaccc agaaaagccc cgttcatgct atcgggattc
    11761 tgaataacgc gatcttgatc aaaaaaaata tattctgctg caagagctgc gcccaaagcc
    11821 ccgccagcat caccggcagc gggttgaata aagagattgc gaaacaatcc tgatttcagt
    11881 attttcccgt ttgcaacgca gttaagcgca acaccaccgg caagtacaag gttttctgat
    11941 tgagtgcatt tcttcgcttc agctgccatt cgcagtatta tgtcttcggt aacctcttgg
    12001 atggctaatg ccagattgca atggtgttgt gcaatggggt cttcgctgtg cctgatacgg
    12061 aacccgaaaa gtttttccca tgctgctaag tttatcattc taagccctgt agcatacctg
    12121 aaatacttct gatgcaggta cactgatccg tcttgattta cggcaacaat ttcgcttctg
    12181 atcttttcga tgaacgtgag ggtttgtgca tcgcctgcgt tcccataggg tgctaggccc
    12241 atgagtttat attcacccga attaaccaca aatccaagaa aataagtaaa agcagaataa
    12301 agcaggccta cagaatgcgg aaatcgtaat tctttcaaaa ggcttatcga tttttcgcgt
    12361 ccatggcaaa gcgatgccgt tgcccattct cctgcaccgt caacggttac aatggcggcc
    12421 tcgttgaatg gagaagggta gaaggcactt gcagcatgcg aaaggtgatg agaggtgaaa
    12481 agcaattgca gtttacgctt tttatatggc ataatttccc tgagctcgtc gcgcaatagt
    12541 tttttcatga acagcttttc ttttgtccat acaggaatgg cctttacaaa cgaaacaaca
    12601 ccccttggtg aataggcata ataagtctcg agcagccgct cgaatttaag aaaaggcttt
    12661 tcgtaataca ccaccgcatc gagatcgtca atattcaggt tgcaaaattt caggcaaaag
    12721 cctacagcct gtgccgggaa agaattgtca tgtttcttcc gcgtaaaacg ctcttcctga
    12781 gcggcggcga caacctttcc atctatagta atacaggctg ccgaatcgtg ataatatgcc
    12841 gaaatgccga gtattttcaa ttcttgtctt tatctgaata caatgtcatc aatcacattt
    12901 gcttgcgcca attcgtttaa ccttgaaata agtcccgatt ttatcatcat caattcgtgt
    12961 cgcacaatgg atgatgagag cgtaacatag agtttccgct tatgcacgta aaggtctttg
    13021 gtactgttgg cgatcatttt gccaactgcc gtttcccaag aattaaccaa tctggccgat
    13081 actatgccgt actccagatt attatccgaa atatactgct ttatcaggtc tgaaatcgat
    13141 gtggtactca ctcttttcat agctcaaaat tagttaaaag ctgaatacat gcaattgata
    13201 aagcgaagat attgaagcat tatatttatt ttattgaatt gcatcaaagc agctctgcta
    13261 tttatcttta tattcaggtc attgtcttaa tgaaaaatga attaattttg cataaaatta
    13321 ttgctgttca tggaactttc gttaaaacaa atcatagcaa atctgagtgc taccgaggag
    13381 acaactgttc tcgattcggt gaattacctc gccggaaatg ggaagccgga atctgttcaa
    13441 ccgttgatag agtgttattt tattaccttt tctcaacccg taaaaaaacg cattctggaa
    13501 ttgttttgcg atatcaagga ctcaagggct gcagcaccct gggtggaaac gatgatgaaa
    13561 catacgcaaa gtgatgggtt tcgggagttg ctctcctgtt gttggcaatc ttctataggt
    13621 tttcagcatc atctggaaag ctttgcagac atagttgcaa cggccgattt gtacaccagt
    13681 atcgaagcga tcagtgttat tgaaagcaat atagcaaatg ccgggcataa cgaaattatg
    13741 caaatggcct cacaactgag ggattcaatt ccggcaatga gtccggaaaa acaacgcctt
    13801 attgactcgt ttttagcggg ttacgagctt taaataagcc cacttcatta tgccagaaag
    13861 agttttccgg tgcaagcagt tctcggttat tcaccggaat tcagcaatga aagtgggtac
    13921 cgatgcaata ttgttaggcg cttgggcaag cattcctgaa agcggcaaaa ttcttgatat
    13981 tgggagtgga accggcataa tcagtttaat gatggctcag aggagcgttt gcgatatcac
    14041 tgctattgaa attgacgcag atgcgtatga agaatcgttg gtgaatttcg gtaattgccc
    14101 atggactgag aggatttcgg tatataattg caatatcttg aaattttcat cacctaaaaa
    14161 attcgaactt ataataagta accctccgtt tttcgataat cggtgctttg attccggaaa
    14221 caaaagaaac ctggctcgca acaacgagag cctttcattg tcgtctctca taggaatagt
    14281 cgcaaaactg cttacgaaaa cagggtgttt ttgcatgatt tatccacatg accaattaaa
    14341 acaattacta gaatttacga cattaaataa tttgtatatc aataaattaa cctatgtaat
    14401 acctcgcttt ggtatgcctc ctgtccgtgt gcttgttaag tgcagctttc aggaaatgtt
    14461 ctcggaggaa tccgaaataa ttattgaagg ttgtaaaaga ggagtttata cagaagaata
    14521 tataatgctt acgaaagatt tttatttgaa attctgaaag ctagtgtcgg agcaattata
    14581 ggcttatgat ttttcaacgt ttaatttttt gtataacagt tttataagtt ttcatgtgta
    14641 aataaacaat tttttaaaat caatgaacgt gttgaagata caatgattta gacaattatt
    14701 tttttttgaa aattggttag actaatccga aataagggta attttaccgc actaataatg
    14761 atgcaaaaca gatatgaata ataagaaaac cgttcgccct gttaaaaatc aggagccccg
    14821 aaaaaagaac aaaagcaatg gaagaagttt ggttgttaca attattgcat ttgtcgccgg
    14881 tggaggactt ggattaattt tgggcttctt tatttcgggt aaggaagaaa aaattattga
    14941 aacacaatca gatcagcagc agcaatttac cgaaatcagg gctggggggt acgaatttac
    15001 aaaccctttg ctggattgcg agaattttca cccctcagtt tcgaggaatc acgtcagttt
    15061 aaagactcag ctaaacgatt atgtgaactc tgctcttcag gccggattgg ctgaaagcat
    15121 ttcggtgtat tataggtcgc tgaacaacgg cccctggatt ggcatcaacg aggatagttt
    15181 ttatatgccg gccagtatgc ttaaagtccc cctcatgata gccgtctata agaaagccca
    15241 atatgaatcc gggtttttaa ataaaaaaat cgactatacc cgtgtacttg acccgagttt
    15301 tactcaaaat attgttgata atgtgaagat tgaagtgggt aagagctaca ctgtcgaaga
    15361 attggtagaa tatatgataa tgcactccga taataacgca aaagagcttt tattggctca
    15421 gataggcgat gaatttctta ttaatgttat gattgactta ggggtaaata taaaaaacag
    15481 ggatttatcg gttgattttg tttctccgaa agagtattca gctttttaca gaattttgta
    15541 taattcatct tacctaagcc gcgattattc cgaaaaagca ctgaaattac tgagtaaaac
    15601 catgttttcg caaggaatac cgggaaagct tcctaaaacg attaaaatag cgcataaatt
    15661 tggggaaaga ggctttcaga gcaccgatat taaacaactt cacgattgtg gaattgttta
    15721 tttcgaaaat aatccgtatt tgatatgcat tatgacacgc ggcaacagct ttgataattt
    15781 ggtcgggatt atttctgata tttcggagat agtttataaa tcggttaacg aagatatttc
    15841 aagtcagggt aaataggtaa tacccggttc caatttttgc aatatcccga ctaatcactt
    15901 gtattgaatg taaaccccat gtagcgtatg aatccatgct gcattattgt ttactagagc
    15961 aggttttaca aggttttatt catcagaaat ttgctttgtc agcctgcgat aattcgagaa
    16021 aactttagcc ctcgaaataa agccaacata tttgttgtcg tcgtcaacta ccgggagatt
    16081 gtagttaccg gaatcctgaa atttttcagc aaccgcttca accgtgtctt taatattaac
    16141 gaggggttgg ggcataaaca tgaaattact tacataagta gtgtcgtaaa gttcctgctt
    16201 aaaaataata tgcctgatat gatcgagaaa aacaatgcct tcgagttttc cctcgtcatc
    16261 taccaccgga tatatgttac gcgttgatct ttctatcgca aaaatcaatt cgcggagggt
    16321 gttatcgctt tttacacaaa taaagttact ttcaatgagc tggtcaactt tcatcatcga
    16381 aagaactgtt ttgtctttgt ggtgggtaat gatttctccg gttttgctga gttgctgcag
    16441 gaacagcgac ttttggttaa aaagccttac ggttaaatac gacaaggtgg caacaatcat
    16501 aagaggtaca aaaagcccgt aaccgccagt taactcggca atcaggaaaa ttgccgtaag
    16561 gggtgcatga ataacaccag caatggtacc cgccatgcca acaagtgcaa aattggttga
    16621 cgaaacattg aatccgagca gctttaacac atacgacaaa aacaatccga cattggctcc
    16681 gataaaaaga gacggtgcaa aaataccccc gacgccaccg gcgccgaaag tgacccctgc
    16741 ggcaaaaacc ttgagcagaa ttattgcaac aaaaaccata ataacaatcc agatactatc
    16801 ggaaaaagca tagaataacg aattgttgaa aaggtatgag tagtcgccat gcaggctggc
    16861 atttattact tcgtaccctt caccgtagag ccccggaaaa agaaaaatca gcaacccgag
    16921 agcaaaactt ccaataaaaa aacgcgttat ccacgattta atttttgcga acccattgtg
    16981 caagaacata tacactttgg tgaaataaag cgaaaggaag cctgtaagaa ttccaagtaa
    17041 tatgtagtaa ggaatatggc gcatccagaa tgaatcgatc ctttcaacaa aatagattac
    17101 gtcatgcccc agaaaaagat atgaggttag tgtcgccgca accgaagcca gcagcaaggg
    17161 tatcagcgac gacattgtaa ggtcaagcat aataacttca agagcgaaga ccaggcctgc
    17221 aataggtgct ttaaaaatgg cagcaatagc tgccgtgcag gcgcatccga taagtaaaac
    17281 tatctgctta ctgttcaggt gaaacaatcg cccaatattg cttcctattg ccgaaccggt
    17341 agcaacggtt ggtccttcta gtccgacaga acccccaaac ccaatggtca gggcactgga
    17401 tatcaaatgc gaaaacatat tgtgcccctc gatacgtccg tttttctgcg atttactgcg
    17461 cagcaccata gctactcccg gttcaagttt ctttttaaga atttgccgca gaaaaatgat
    17521 taccagaaaa ataccaagtg ctggtgaaat gatgaaaaga tagttctcgt tatcaggcga
    17581 aaaccatgtg gttacaccca tccggatgta gtgaacggaa ttcttgagca aaacagcggc
    17641 aagaccggca gcaatgccgg ttagcacagc aataaacatt ataaaagttc tgtcaggaac
    17701 tttatgtaac cgccagatca ggaatttttt aagaatacta ttaaatcgct cacgaaacat
    17761 aatcatacaa atactgaatg caaatctaat aaatgttcat tatcttccaa caaagaatga
    17821 attccagtga caatcttatt tgtacattat ctcacttcgt aaagatgcat gtttactgat
    17881 actgagtgcg gttaattttt aaagaatgta ctattgcaac aggtttagct taccggattg
    17941 ctttcatcgg tttctgttgt ttcctgggtg ttgcctgtgg ctgaattgtt gttattgaat
    18001 tcgtcaactt tattcattct gtaccccgat aacaccgaca actgcttcat ccgttttaga
    18061 gccaggcggt acaatacaaa cagatagact gcaaaaaaaa tcaggaaata gggcatacct
    18121 gacataagaa taaaatcgta taagccatga agcagaattg ggagaaatat agcggtgagg
    18181 atgagtcgcc tcctatgtgc aggatcgaat tttgcgcggc ccagataata gcccatagta
    18241 acaccgaaaa tggcatgagc cggtacggca gaaatggccc tcaccaatgc agtggaagcg
    18301 ccgccttcaa caacataaag aatattctct accagtgcaa atcccagtga aatgtaaaca
    18361 gcatatacaa taccatcgaa atgttcattg aaatgcttgc ttctccatat gaaaataatg
    18421 aatacaagga atttgaagct ttcttccgaa aatgcagcaa ctacaaaggc gtcccaaccg
    18481 gccagcaaat aaccatccat ccatgaagca ggaaaggata gaatagtttc tgttattaaa
    18541 atcggaatca cagccaatgc tccgaataag acgcttaaca ggagttttcc gataggctct
    18601 ttttcgtatt tatcgcggta atatacgtaa aacaaaatca gaattaccgg tgcaaaagct
    18661 atgagtatta aattaatcat attatactaa ttaaaacgta ttgcttcaga aggacttacg
    18721 cgtgtaacca gaaaagaggg gacaagcatc atcagaacag taatcacaaa agagccagca
    18781 ttgagtaata caatatgcag gaaatttata tttatcggta catatgaaac gtaatacgat
    18841 accgggtcga gacttacaat tttaaacgcc cattgaagca gacagaggcc tattcccaat
    18901 acattgcccc acaacatacc ccttccgata agcatgccag catatataag gaaaacccgt
    18961 cggatgctaa catttgtgct ccccagactt ttcaggatac caatcatatt tactcgttcc
    19021 agaataatca ccagcaaacc actgaccata ttaaaccctg caacacttac cataagtatt
    19081 agtatcaccc aaacgttcat atccagcaaa cccaaccagt tgaatatttc aggataatcg
    19141 tcctttatgc tgcttacctt cagaaaattg ccattctcat caggaatatt acttactaca
    19201 ctttttactt cactacttat taaatcaatg ttttttaagt cgtcgatgag aatttcaaat
    19261 ccggttacct gtttttcgtt ccagtcgttc aatttctgaa tatgtcttgc atctgcaatg
    19321 ataaacagct tatcaaggtc ttgcagctgg gtgtcgtaaa ttcctgaaac aacaaacttt
    19381 ctcatgcgtg gaggatcctg aataaagtag ctcgatacct tatcgccaac cttaagtttc
    19441 agaatgtcgg caagatattt ggaaacaagt atttcgttgg tttttcctgt gtcgcaaatg
    19501 gcaggaatgc tgccctcaac catataattt tcaaaaaaac tgaaatcaaa actcttatct
    19561 acgcctttta ttacaacccc ttgcagatag taatcggttt taaaaatacc agcttttgta
    19621 acgaatggct gaatgtggcg cactcctttg atggacctta cctgattaaa ccagctttgg
    19681 tgcaagccaa taggtttggt ttcgtacgaa gtattggtgt cgtaattaat aacacgtata
    19741 tgagaaccaa acccgatgac ctttttactg atctcattct taaaaccggt tacaatggcc
    19801 accgacagaa gcataacagc caaaccaata gcaatgccag cgattgcaat gtttactaca
    19861 ggtcggctga atgaggctcc atgtttttgg cctttcagca ttttgcgagc tatgaacaac
    19921 tcctgtttca ttcgattgta aaattaaaat acttaatcga ataatcatca cagcaaaact
    19981 gttaattatg tattagtctt gaataacgac atttattaat cttttcaata gttataaaca
    20041 ctaaaattat cgtatttata tttatatatt tttgttaaaa aattaactca atggacactg
    20101 tttcgaaact gcaaaacatt gcaacacaag taaggagaga tgttctaaga atggtgcatt
    20161 caaaaaactc aggtcatccg gggggtgcgc ttggatgtgc cgatttgctc actgcactct
    20221 attttgaagt gttgcatcag aaagaggttt ttgatatggc tggaaataac gaggacatgt
    20281 ttttcctgtc gaacggacat ttatcggcgg tttggtatag tgtcctttcc cgcaaagggt
    20341 atttcccggt aaaagagctt tcaaccttca gggcaattga ttctcggcta cagggtcacc
    20401 cggcaactgc agagcacata cccggtgtaa gagtggcttc cggttctctt ggtcagggtt
    20461 tatcggtagc agcaggggct gctctcggga aaaaaatgag tggtgataaa aacatcgttt
    20521 attgcctttg tggcgatggt gaactgcagg aaggacaaat atgggaagca gctatgtttg
    20581 caactgcacg ttgtattgac aacctcattg ctattgtcga ttataatggt aagcaaatag
    20641 atggacctgt tgatagtgta ataagtctgg gaaacctgaa agccaaatgg gaagcttttg
    20701 gatggattgc tctcgaaacc aatggtaacg atatgcgctt gctactgaat acacttaatg
    20761 aagctaaaca caataccggc aatgctaaac ccgttgtaat actaatgaaa acaatcatgg
    20821 gcatgggagt tgattttatg atggacgatc atcgctggca tggttcagca cccgacaata
    20881 aacaactgga acttgccttg cagcaattac cggttacttt aggcgattat taatatgcaa
    20941 aatttgaaaa aaattgtatt tatactaatt acgcttttaa tctgcacagc aaaccttacg
    21001 gtaaaagcgc aaaacaaccc gcctcttacc cgggtgcttt ttatactcga cggttcaagc
    21061 agcatgctcg aagtgtggga caggggaata aaattctaca ccgctcaaca acttcttaca
    21121 gaactactcg attccttatc agggttgcct aatcaggata atatacagta tgccctgcgt
    21181 gtttttgggc accaaagtgc tgcaccaccc gaagattgta acgacacaaa acttgaagtt
    21241 ccttttgggt ataataattc agaggccatc attgcaaaga ttgaaagcat taaacccaaa
    21301 ggcacaacac ctatagcccg aagccttctg gattgtgcaa acgatttccc cgattgcgac
    21361 aattgccgca atattgtgat attaatcacc gatggaatgg aacgttgcgg gggagacccc
    21421 tgcgaagtga gtattgcttt gcaaaaacag ggaatcattc taaaacctta tatcattggc
    21481 attcggctcg atacccaaat tgccgactat ttaagttgta tcggtaacta ttttgatgtg
    21541 ggaaacgctg atgagtttgc cgatgcccta aatacggtta tctccgaagt taccaatctt
    21601 acttcggtgc aaatcaatct gctcgatatt tacggaaacc ctacagaaac aaacgttgcc
    21661 ctgagctttc atgataattt gtcgggaaat atcagatata cgttcatgca taccatgaac
    21721 gacaaggggc aacccgatac attgcacgtt gatattatgt cggtgtacga ccttaaagtt
    21781 catactatcc ctccggttta cctcgatact cttattgttc agcctaaaaa actcaatgta
    21841 ttcgaagtgc ctgcccctca gggtagctta aaagtggttg tcgacgggaa cgaccccgac
    21901 gataacgacg tgaaatgcat tgtgcggcaa accggtctaa tggaaaccat tcacgtgctc
    21961 gatgccgaaa aggaacaata ctatattgtt ggattgtata acctcgaagt gcttacgctt
    22021 ccacgcatat atattgatgc agttgaagtg aaacaggatg tacagaaaac cctgcgcatc
    22081 gcacagcccg ggcgtgttat aatcaacttc aaatcgccgg tttacggatc gctgcttttt
    22141 gagaacggag atgtacttac caatatttat gattttatcc ccgacagggt ggtgtataac
    22201 ctgaggcttc aacccggata ttatagaata atatttcgcg ataaaaacca aagaaaaacc
    22261 gaaaaatcac tggaacaaaa agtcagggta aaatcaggag aaacctatat ttttaattta
    22321 taaaattcat gacaacctac acctataccg aaactaagga cacccgctcg ggatttggag
    22381 ctgcgctgag ccaattgggc aaaacgaatc catcggttgt tgctttatgt gccgatctta
    22441 ccggctcact gaaaatggat gcttttgcta aagcgcatcc cgaacgattc tttcaggttg
    22501 gtattgccga agcaaatatg attggagtag cagctggttt aagcataacg aaaaaaatac
    22561 cctttgcagg gacttttgcc aattttgcca ccggaagagt atatgatcaa atcaggcaag
    22621 tagtggctta tagcaataaa aatgtaaaaa ttgccgcctc acatgctggt ctaactttgg
    22681 gagaggacgg tgcaacacat cagattctcg aagatatcgg cttgatgaag atgctaccaa
    22741 acatgactgt aattaacccg tgcgattata atcaaacctt tgctgcaaca atagcatctg
    22801 ccaatcatga agggcctgta tatctgcgtt tcggcagacc aaaagtgcca aactttacgc
    22861 ctgcagcaca ggaatttgtg attggaaagg ccattttact taacgaagga aaggatgtga
    22921 gtatttttgc aaccggtcat ctggtgtggg aagctttaca agcatgcgaa atgcttgaat
    22981 ccagaggaat cagtgccgaa atcatcaaca tacataccat taaacccctc gatacagtag
    23041 ctgtgcttaa gtctgtaagc aaaacaggag cagtggtaag tgccgaggaa cacatgaaga
    23101 atggtggact gggcgacagc attgcccagt tacttgctga aaattatccg cttccacagc
    23161 agtttgttgc cgttgacgat acatttggtg aaagtggaga gcctatgtca cttatgaaaa
    23221 aatacggtct ttcttctgat gctattgtaa atgctgcaat taaagcaatt ggcagaaaat
    23281 gtaaataata agctatttag ttcaggcttt gctttttacc aacttcgttg tattcttcga
    23341 ttagccctgc acaaatccac tttgccagaa tatcgcggtt gtcgggatcg agaaatcttt
    23401 tctgatcatt cacattcgac atattaccca gttccataaa tacccctaca ggttttgtct
    23461 tccgtagaac cattaagctg cgcggactca cagaaccatt gtagaccctg ttcggctgat
    23521 gttctttgta ttttgcttca aatgtactta gcatgatttc agccaaacgc tgacctttgg
    23581 tgcttgtggt atgataatag aaaaatacat caattttttg cgattcaggg cgggaatcca
    23641 gatgaatttc gattacgcgc tgatgcacca aagcgccgta gctatcatac aaattgttta
    23701 ctgcatatac ccgctgaagc agcctttcgt tgtgatcgcg cggaatactc ttatcagggt
    23761 aacagatttc atcggtatcg tgaactagga atttctcatt gcggatactg tcgttcgggt
    23821 caatcacaat cattattgcc acaccggcat gcgattcaat ttcacgcttc aaccgaagcg
    23881 ctatatcgta agcatattca tcttcagtta tcatattacc gtccacggta gcaaccgcac
    23941 cggggtcagg gccaccatgc ccgggtatca gatagaaaac cgttcccgat agcaccgaat
    24001 caatgcaaac tattccgggt acactgtcgc tcacaaccgg cacaataata ctatcgggct
    24061 tgcagggcag ggcatatatt ttccccgcaa tcaatgtgtt ttccggacct aaatattttt
    24121 tatttatcct cacaaaatca ctgaagtagc ttgcttcgta atggttgcgc tttagtaagg
    24181 catacacgcc ttcgcctttc aacgcttcca ctttcaccgt atcgcactgc gaaatggcaa
    24241 ttcctgcgtt gcatagtaat ataatgcatg tcagaagacg aaacatagca attaaattac
    24301 tacaaaaata aaaaaatgac agaatgaaaa agaagttgtt tcgctgataa tttttattta
    24361 gaatttttat taaaaaccaa gaaaaatttt aacaatatat ttagaccggc gttatttagt
    24421 tgtttgtaat tttgacactt agcaaaggaa gtatttctta attatggcga gtgtgttaca
    24481 accaattgca caattttatg actttattat taaaatataa tatttagaac tgcaaaatgg
    24541 gaataactca aataacatat attaattttt aacctttaaa acttaaacat atgaaaaaga
    24601 taggtgtaac aatcatggca atttttattg ttggctctct ggctatggct caaaccgtaa
    24661 ataaaggagg gcagcacaac aacacacaga acggaaaatc tgttgaaaaa gcttctgttg
    24721 ctccgactca gaaattaaac aaccattccg ctgagtttcg gggaagcaca cctgatgcag
    24781 ttcgtccggt tgatcaagcc cagcaaaaag cattt
//
LOCUS       DAOOQP010000031        24602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2460_length_24602_cov_5.681998, whole genome shotgun sequence.
ACCESSION   DAOOQP010000031 DAOOQP010000000
VERSION     DAOOQP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2460_length_24602_cov_5.681998"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLK75_05115"
     CDS             <1..291
                     /locus_tag="PLK75_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOY38662.1"
                     /translation="LTSLSGGEYQRLLLAKQLMHPQKQKCLYIFDEPAAGLHPFDIEK
                     LNVIFLNLAENGHSVVIIEHNPTMTAIADQVLELGPVGGPEGGYILKNHTSE"
     gene            465..1277
                     /locus_tag="PLK75_05120"
     CDS             465..1277
                     /locus_tag="PLK75_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOY38663.1"
                     /translation="MILDETFALLKTRYENRFNSTRIEKVQIGVCFAAVQLSSGYCGL
                     SSVDSASVSCCTGKRIRENLPFTPGNICGQKLLDLFSCPLESRTLDIVRMAAMNAVSQ
                     ELIASEDYTIVEDKDPIELVDLQSNKIICIVGAFHSYMKTISQTQSTLVVIEMNELAI
                     PDEYKQCYVPASKAASVFPEADTIIITGSTLVNRTLDFVVESIPSGKQVILVGPTAGL
                     IPDVLFRHGIQVIGATRVADPERVMQAIAEGAAGYHLFRNGAQKICIMNNAK"
     gene            1267..3021
                     /locus_tag="PLK75_05125"
     CDS             1267..3021
                     /locus_tag="PLK75_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOY38664.1"
                     /translation="MQNKTLSDTWLKASVLGCLWASSEIVLGSFLHNLKVPFASNFLT
                     AIGIILLISVSYIWKDRGLIWRSGIICALMKAVSPSAVILSPMIAIFFEALLLEFAVT
                     IFRKNIFAYLVGGMLAMSWTFVHKIAAYLIIYGFNLVELYRNLAIFAQKQLHIQIENI
                     WSPVIVLWLLYGLMGIIAAILGIYIGKKAIKTPLQNNLFRGNNVYRNPAPKPVQVFKH
                     SLMWLIVNGISMIGVLLLMNYAHWIWWSSAGVAIACVWAIKYRNVLHRLKKPGFWLFF
                     VAITMISSILFSKFSANTVSWIDGLMIGLQMNVRAVIMVMGFAVIGTELSNPVIRNFF
                     ARTPMRQVTPALETAFETLPYAISSLPDIKMVFRKPVSIIQQIVMQSEYLLEKIKLKQ
                     FGKENVVIITGEIHQGKTSILQALIPMFKEQNLVVGGIISPGIFEDNMRIGYDIIDVA
                     TGERRVLSRTTGSENMIQIGKYFLHDEGLVFGTTALSVENNWNSNMIVVDEIGPMELD
                     NQGWAQSVGELLQAFDVPMIFAVRKGIIEQVTEEWAITDPLIIDAEKCDTTMAFSLIS
                     NFFKNKKMNTVSEHYVKE"
     gene            3082..3669
                     /locus_tag="PLK75_05130"
     CDS             3082..3669
                     /locus_tag="PLK75_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOY38665.1"
                     /translation="MSDNFSTIILASGMSGRMGEPKALLRWDSSFTFLEKIVDVFVRA
                     GSSNIVCVVNKAAEPYCIGLKLPSCVSIATNNHPEWDRFYSVKTGIQHVKDTDYCFIH
                     NVDNPFITPEIVKEIFASRMPGAWCSPVFEGKGGHPVLLSKTIYSRITNKMPDETKLK
                     DFLFPFPRIIVETDSDCILRNINTPDDYKRLVAQQ"
     gene            complement(3679..4449)
                     /locus_tag="PLK75_05135"
     CDS             complement(3679..4449)
                     /locus_tag="PLK75_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOY38666.1"
                     /translation="MKNLLLKISDYYKDTRASALCIVTSMSGSTPGKAGAKMVVFSDG
                     AIEGTVGGGGIEKQVIADALEVIRTQSTLFREYNLQTDLAMTCGGKLCVYIEPIAKPA
                     KLFIFGAGHIGKVLAGYAPEFGFETTLIDWRSDIFENPDAIHYTQVCKPYLEVASEIQ
                     PDSSTYCVIVTPNHDMDEEILAVLGKKSMAYLGLIGSKVKIATLRKAFLQDNILTEEE
                     LNRVDMPIGIKFNAIAPAEIAISILAKLIDVKNTKDKK"
     gene            complement(4569..5090)
                     /locus_tag="PLK75_05140"
     CDS             complement(4569..5090)
                     /locus_tag="PLK75_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOY38667.1"
                     /translation="MSQTRFIQNEQHYTEVVELFTAVKRSIWIATADIKDLYVKKGTK
                     VVPLLELLSDLITRGVSVRLLHASEPGRTFREDFDRYPALIEGLERMLCPRVHCKLIL
                     FDNKTAYIGSANLTGAGLGMKSSDNRNFESGILTSDPELVDAAVNQFDRVWMGAHCSK
                     CKRKEFCIDPISK"
     gene            complement(5087..6049)
                     /locus_tag="PLK75_05145"
     CDS             complement(5087..6049)
                     /locus_tag="PLK75_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOY38668.1"
                     /translation="MPETKKKIVFIINPVSGGRSKTRVEAAIKKYLDRDLYDYQIIYT
                     AYRHHAIEIARNESEKGTDVVVAVGGDGSANEVARGLVNSNTVMGLIPVGSGNGLAHY
                     LRIPVIYRKSIEILNNHNIKRIDTALLNDTMFISIAGMGFDALVAEEFAGSKQRGFVP
                     YLRIIKRNIFSYKPELYHIRFKDKDIKTKALFVSFANSDQFGFNASIAPVARISDGLI
                     DLCIVHPIPMYKVPHLANMLFLKIINRSQYVEIYKTDSAIVETENEVPCQFDGEPSSR
                     CKRVQISIVPRSLNMIVPYKKTDPRVYLRGMRQSMKLQSKLSLK"
     gene            6160..7584
                     /locus_tag="PLK75_05150"
     CDS             6160..7584
                     /locus_tag="PLK75_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38669.1"
                     /translation="MNKLLISGFIIAMVCNTLNAQQSLLGDNPIHISFSYCNTDNVEK
                     LLRKNKTISYSDLQIQELRMEIWNQNARIDKEQQELINRRNELISVRQQIVQLGNPEL
                     ITARINEKKSSIDKINADIRNNLSGLGYKGLYVFACKISSSYDTEKQLRDQAMKFIIP
                     VAVEKTNGSYIASVTELRRKEGYDDTLYRYISEVTQGTSNVEAEIINRMNRKGGYYIY
                     MAKLNTKPLQKEVKTLTAADGNNPEYVVVFDALRDKNMTKKILEVGASKDDLKKLEQE
                     IDNNKALVLAENARIEEIERNYILSGNERINQTLREIEKLNAEYKSNIEALQSLMQVY
                     TPNIKLDEKSPKNSITAVIKEIDDKIAALDSGIMEKESQRLYGDFQILVTSGSDYFRK
                     IAEESIASKDRLTEMYAKANNYVQFVEMENDDYRKAVGNEEVYQRYFENMWVFPEPGD
                     GDFFRVTVIVKFSLQKTKPAWKVW"
     gene            complement(7618..8748)
                     /locus_tag="PLK75_05155"
     CDS             complement(7618..8748)
                     /locus_tag="PLK75_05155"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOY38670.1"
                     /translation="MKEIPMVDLKSQYLKIKDEIDNGIHEVIDNTAFIGGNAVKQFQQ
                     ELAAYLGVKHVVACANGTDALQIAMMALGLKPGDEVITADFTFIATVEVIALLGLTPV
                     LVDVDPQYFNIDTEKLKKAITPRTKAIVPVHLFGQCANMEEIMKIAGEHNLFVIEDTA
                     QALGSHYLFSNGNSAMAGTIGHIGCTSFFPSKNLGCYGDGGALYTNDDNLAYLIQSIT
                     NHGMRVRYYHERIGVNSRLDSIQAAVLRAKLPHLDNYIKARIRVADYYAKAFSSCKNL
                     IVPARNTYSLHTFHQYTLILKDTDRAALQNHLREKGIASAVYYPVPLHKQDAFQGYSY
                     CADDYPVTDRLCETVLSLPIHTEMDDSTLKYISDSVLDFFIR"
     gene            8787..9812
                     /locus_tag="PLK75_05160"
     CDS             8787..9812
                     /locus_tag="PLK75_05160"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY38671.1"
                     /translation="MKTIFNEIYVNLPHNCVEMNLQNYLISLLYEHNCVILPGFGGFV
                     VNYKEAVIDFQNQKLSPPSRIVAFNQNLNVNDGLLADFIARSEKITYDEALHKIENFV
                     AAMTDDLGRMPSVALSGIGSFAMNHDTLEFRPEAGQNFLRDSFGLESFHFPLLKTAKP
                     REVIEEKAKTVVSTVTVNRRIKPSWAIGIPAAAAIFILAVMFFNQDNLKFNIQQNTEM
                     NPLYVGPTKNIDDKTNTVSAEAVLPQAEKPDATYNQPAEVQSVDVAAQNELKVHVIAG
                     SFSQQENALTMAESLKTVGYNAQILHIEPWYKVSIKAFATPEEAAAELNALKQATGNN
                     ALWVMTN"
     gene            9971..10576
                     /locus_tag="PLK75_05165"
     CDS             9971..10576
                     /locus_tag="PLK75_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY38672.1"
                     /translation="MMSDQEIIKGCIKSHSDAQAALYNRYAPVLRGICMRYTGNKDDA
                     EDVLHDSLIKALMAMKQYKGNGSFEGWLKRIAMNTCISYINGKNKSGVSSVNRVVENE
                     ADDPEETEGNQGLIERFLNSGIEKSDLLQAVAGLPDGYRMVLNMYVIDGMPHHEIAAQ
                     LGISINTSKSQLSRARNLLKSRLLGMIQNNEFNVHVNEKCY"
     gene            10560..11162
                     /locus_tag="PLK75_05170"
     CDS             10560..11162
                     /locus_tag="PLK75_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38673.1"
                     /translation="MKNAIDNKLDNVLREELGAIEIAGPPAGLTSFISKIPKTGLSYV
                     TQIIIITVTSMAVVFTGIIMWQAGANHAYQKLTAKELHNVSEKQLFRVNYTSKGALPL
                     EDSNERLAFATNNTVQNRHTGAAISTTKNDTYIINESEANARTIMPAGTENNDFKKDV
                     SAPYFCDTVVVYDTVVVVDTVLKFRQQKIIVKSKRKNTRR"
     gene            11163..12092
                     /locus_tag="PLK75_05175"
     CDS             11163..12092
                     /locus_tag="PLK75_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38674.1"
                     /translation="MNSQSKYLYFIALLILLLPAGELFSQQTDTVKVQIRKVVYDTVF
                     TEVEIPVYDTVYKERFISSLSFSPAIGAGVGVNRFGKDTDSTALNFLNRTYAESWFYS
                     VGTGFTATSGRFGIGTGVYYTAMDQKFIYSHSTFNYDTITYYNTEIHLEVQTDTVWFD
                     SINYQITTISNYTLDSVPYDSVLVHTHDTSLNYTNRYRMIRIPLLLSYSLIDKKGFVL
                     NFSIGPVLGIMFRNDSYSLGLDLYPVKNEKKIYFIPYIYGAVGLEYAFCPGISLGCDL
                     FCCFPLKSIGNSSGVYWYNIDNYGVALKYLYYF"
     gene            12162..13565
                     /locus_tag="PLK75_05180"
     CDS             12162..13565
                     /locus_tag="PLK75_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38675.1"
                     /translation="MKKIILFSLLISSFIILPQMLRSQNCYLQASYEANGLDVNFAAQ
                     LYDLTVNQQDSSFQPIQGQYIWVIDNATLSGQNLEFSYNSYGQHVVTVTGSGMGCSAT
                     TTLIVTLDENNPIDSTGNFVVLISGDSYSSLDCDADINAYVIGGIAPVSFNWNNGVTS
                     SLLNDVCAGEFCVTAVDANGTTAESCFTVGYGNTDTLPNDSTNYGNIYVSIEYYSNSL
                     DDCGYSAWASVYGGTPPYFYQWNTGQNTSQVAELCVGQEICLMVVDIDGNSATACALI
                     LADSVYNTDTVLNGGDPWESTFEWQGDSLFADAQIVHYWITNTEICFVWALIYENSYQ
                     ETMTVCYPLSDSLNSGWYEVILHVIFNNGQKANISFIDNVYLEPSDLSSNPDNENILD
                     VTAYPNPVGDMLNISVSKQGNSCSISVFDLSGKLIYKSDYSGNELIRIETSNWENGMY
                     FLEVRSDNEKSVLKIVK"
     gene            complement(13633..14343)
                     /locus_tag="PLK75_05185"
     CDS             complement(13633..14343)
                     /locus_tag="PLK75_05185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOY38676.1"
                     /translation="MEENKVNAMPRIGDRAPEFEAVTTQGKISFPGDYKGSWVILFSH
                     PADFTPVCTSEFMTFASMEKKFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKD
                     VEVTFPLIEDITMEVATKYGMMMPGESNTKAVRAVFVIDPKGIIRTIIYYPLSLGRNF
                     DELYRVVLALKTADEFSVATPADWRPGDDVIVPTAGSCGVAKERMLSKDEDVKCYDWF
                     FCTKKLDKEKVMKAIIKK"
     gene            complement(14463..16541)
                     /locus_tag="PLK75_05190"
     CDS             complement(14463..16541)
                     /locus_tag="PLK75_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOY38677.1"
                     /translation="MRVYITLALLLFAAVLFAQSNENKRTEIVARNFYYEQANAFKSV
                     HYDDISVVSTETRMHNGKAVYTICAMNPSGFVIVSESEKVVPVLGYSFAEAQVPAEMN
                     SSCATWLDNYAVQIAHVIDNQTLVDENIAAMWSHLQGESSSLVKSVKTGVTPMLFTQW
                     NQGLYYNAMCPADGAGPGGHVVTGCVATALGQLLNYFRYPEQGTGSYGYQHDDYGWIE
                     VDFSQQNYNYDQMAVELTDFNDDVARLINNIGVSVDMNYGPDGSGMWNHKGAYTLHTY
                     FGYDDETTYLFRDSLPEDFDWKGTLIQHLDQRIPLYYAGWSDYEFVSGHAFIVDGYQT
                     EDYYHFNWGWGGSYDGYFYLDNLTPGGSDFTLLHEVIVNAVPSGIYPQGCQSQKILTT
                     TAGTIDDGSGPLFDYEAGTECEWLIQPNDSANGIEFEFLKLQTDPNDFITIFDGPTDE
                     AQVLGVFSGNTIPATFESTSDEVLIRFVTDNDLNGDGFLLSYKGIKPVYCPLVTHISE
                     PVGTVSDGSNQYNYQNNTTCYWYIEPENAQSITVEFTDFDLEPVYDYVKILNQSSQTV
                     ATYSGSTLPDVLTIEAYKITVNFKANGNTRAGGFSLNYSTQFVGNPIGATENISVFPN
                     PADNFIEFRTGNMAQPIQIELYDVQGKLLLQQFLPEDHHIDIGHISEGMYIYHLYSQD
                     YTYQGRIVVQ"
     gene            complement(16617..17861)
                     /locus_tag="PLK75_05195"
                     /pseudo
     CDS             complement(16617..17861)
                     /locus_tag="PLK75_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(17861..18952)
                     /gene="pdxA"
                     /locus_tag="PLK75_05200"
     CDS             complement(17861..18952)
                     /gene="pdxA"
                     /locus_tag="PLK75_05200"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOY38678.1"
                     /translation="MDKIRVGISHGDINSISYEVIIKTLSDTRINDMCVPILFGSPKV
                     VAYHRKALNIENFSLNQVRSAEEAHPKRSNIINCIDEEVKVELGKSTPEAGAASYASL
                     ECAVKSMQQGKIDVLVTGPINKHNIQESGFHFPGHTEYLAEKFETKEHLMLLVSGKLR
                     VGVATGHIPVKDIATTLTKEKIVSKLKIFNESLKFDFNVRKPRIAVLGLNPHCGDMGV
                     IGTEESDIIAHAIEEARNHKIMALGPYPADGFFGSDNFTKFDGVLAMYHDQGLAPFKA
                     LAFDTGVNFTAGLPIVRTSPAHGTAYELVGTGQASEESFRNALYLAIDIFKNRQQNKE
                     LMKNAMKDHGHARDFIGSDDDVPTDENDL"
     gene            complement(18995..20449)
                     /locus_tag="PLK75_05205"
     CDS             complement(18995..20449)
                     /locus_tag="PLK75_05205"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOY38679.1"
                     /translation="MKRNIPLIKSVSTVPVDDPVVYLIPENFDCNALSFTKTELAYVK
                     SQLESKETLICINSYFKWSFIVVAPSSGGEAYTVKEALRIKGYEVCNLINKYKINLIH
                     IVDFTRELSFAVAFAEGLVLSAYTFTKYFTDKDKKKQALEQINIKSENISDKSIEMIT
                     ILAESVYIARDLVNEPLSYLTAHKLAECIQATGKDAGFSVEVFDKKKIETLRMGGILA
                     VNRGSKEPPSFSVMTWKPENAINSKPYVIVGKGIVFDTGGLSLKPTQGSMDSMKSDMA
                     GAAAVTGCMNAIARAKLPVYVIALVPATDNRPGENAYVPQDIITMFNGTTVEVLNTDA
                     EGRLLLADALAYAKKYDPEAVIDLATLTGAAVVAVGTIAIAGMGNNKELLKKLVASGN
                     ETYERIIEFPLWEEYDEYIKSDFADLKNVGGKWAGSITAGKFLEHFTDYPWAHLDIAG
                     TAYLDSPDKYRGKAGTGNGVRLLFDFFTNKCNIA"
     gene            complement(20456..20815)
                     /locus_tag="PLK75_05210"
     CDS             complement(20456..20815)
                     /locus_tag="PLK75_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38680.1"
                     /translation="MKTGCLECGEKLTGRVDKKFCSDQCRNTYNNRLNQDSTNMMRNI
                     NRILKKNRRILESLNKKGKTRVTRQRLMDEGFNFDYLTNVFTTKTGNTYFFCYDQGYI
                     QNENDFYTIVKRQDYVN"
     gene            complement(20925..21674)
                     /locus_tag="PLK75_05215"
     CDS             complement(20925..21674)
                     /locus_tag="PLK75_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY38681.1"
                     /translation="MINGKKLVIVLPAYNAESTLAQTYSEIPFDIVDDVILVDDSSRD
                     NTVGIARQLGIRNIIIHEHNTGYGGNQKSCYNKALEMNADIVIMLHPDYQYTPRLIHS
                     MAYLIANGVYQVVLGSRILGRGALRGGMPLYKYIANRLLTFTQNLLMHQKLSEYHTGY
                     RAFSSDVLRKISYNLNSDDFVFDNQMLAQICFAGFEIAEITCPTLYFEEASSINFRRS
                     IKYGMGVLKTSLQYFLQKIRVAKYRIFTVNR"
     gene            complement(21671..23833)
                     /locus_tag="PLK75_05220"
     CDS             complement(21671..23833)
                     /locus_tag="PLK75_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38682.1"
                     /translation="MAKQQPIKKKIITDPKKVGKPLPIPAVKANPYAMIVIIVLGAML
                     YANTLTHDYALDDAIVIYDNQFTSKGIAGIPDIFRYDTFTGFWMTSYPGVSAEKIQED
                     KKLVAGGRYRPLSLATFAMELQVFGDKSTLKGENPKITGRPYISHFNNIILYILTTLL
                     LYKILVRLIPPKPETRWYISLPFVVSVLFLAHPIHTEAVANIKGRDEILTLLGSLLAL
                     WFTLKYLDTRKFINLIFSGVSLFLGLLSKENAITFLAVIPLTVYYFSKHKFVKTLGAY
                     VPLLIAAGVFLLIRYSILGGGRGEKEIAEELMNNPFLYASVGEKFATIFLTLGKYLQL
                     LFFPHPLTYDYYPKQIPIVGWNDWRPVVTLLAYLALGFYAIWGMIKKRDLISYSIWFY
                     IIPLSVVSNIFFPVGTFMNERFIFISSIGFCILMGWIITGPLLQLSETLKMKATSLSI
                     PLLCILLVLYSGKTFSRNKAWENDFVLFTTDVKTSYNSAKSTCSAGGKLIEQAQKSEI
                     KKDTAIHNQYCRDAIKYLEQSIQIYPEYVDAINLLGNAWYEYTTNSVKALGCYTKVLS
                     LKPYHGIALNNAKIIIMNSYGLLNAGKSSNTPEEILDAIQKLDKIKPGIPELYHLEGT
                     IYGKYLQNIDTAIYYLSKSASLKNPNAGLYMDLGVAYGIKGNLPSALENFLKSIELNP
                     SDAESWYNAGVTYYQMGNMAKANEYIGKSNQLKQQTNK"
     gene            24004..>24602
                     /locus_tag="PLK75_05225"
     CDS             24004..>24602
                     /locus_tag="PLK75_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38683.1"
                     /translation="MNKKISLVLLWIATILPAIAQQNIAVLGEIDVLQKGDPVSLLRV
                     DGSDETVAGAVYSDTNFEMNFQASQSSLYMFYYGDEDGFLIVLHPGDTLKIGINKNEI
                     VMEGSYENKMNSFVMMESNLLNEKYNELYEEYLSTEDIDKQVKINEEIDTLVARYNAL
                     IKSFVRNHPGSLLSLIYLEELNIDEDFEFFLSSCDSLMI"
BASE COUNT         7113 a         4923 c         5239 g         7327 t
ORIGIN      
        1 cttacatcac tgtcgggtgg agaataccag cgtttgctac tggcaaagca acttatgcat
       61 ccccaaaagc aaaaatgcct gtatattttc gacgaacctg ctgccggact gcatccgttt
      121 gatattgaaa agctgaatgt aatatttctt aacctcgctg aaaatggcca cagtgtggta
      181 attatagaac ataacccaac gatgacagcc attgccgatc aggtgctgga actgggtccc
      241 gtcggcggcc ctgaaggagg gtatattctg aaaaaccaca catctgaata aaatattctc
      301 tagccaaagc cttgataaat attaccgatg gccattaggt tcaacatttg gctttattga
      361 ttgattttac aattgaatca gcaataatgc tatcaatata ttacctttgc taaaaaccgg
      421 attgcagata gaatattctg accagaattt aatgggagtg agctatgatt ttggatgaaa
      481 cctttgcttt attaaaaacc cgctacgaga accggttcaa ttcaacacgc atcgaaaaag
      541 tgcagattgg cgtttgcttt gctgctgtgc aattatcgag tggatattgc ggtctgtcga
      601 gtgtagattc cgccagtgta agctgctgta ctggaaaacg gatcagagaa aaccttcctt
      661 ttacaccggg aaacatatgc gggcaaaaac tacttgactt gttttcatgt ccactggaat
      721 caagaacgct cgatattgta agaatggcag caatgaatgc tgtttcgcag gaactgatag
      781 catcggaaga ttataccatt gttgaagaca aagaccctat tgaacttgtt gatttacaaa
      841 gcaataaaat aatttgtatt gtaggcgcat ttcatagtta catgaaaact atttcacaaa
      901 cacaatcaac gttggttgtt atcgaaatga atgagttggc aatccccgat gaatataagc
      961 aatgctatgt tcctgcatcg aaggcagctt cggtttttcc tgaggctgac acgattatta
     1021 ttacgggatc tactttagta aaccgtacac ttgattttgt agtcgagtct attccttctg
     1081 gtaagcaagt aatccttgtg ggtccgactg cagggttaat tccggatgtg ttgttccggc
     1141 atggtattca ggttatcggg gcaacacggg ttgccgatcc ggaaagggta atgcaagcta
     1201 ttgctgaagg tgcagccggt taccatttat ttagaaacgg agcgcagaaa atatgcataa
     1261 tgaataatgc aaaataaaac actgtcggat acatggctta aagcgtcggt gctcggatgc
     1321 ctatgggcat cttccgaaat tgtactcggt agctttttgc ataacctgaa agtgccgttt
     1381 gccagcaatt ttcttacagc aatagggatt attctgttga tttcggtgag ctatatctgg
     1441 aaagacaggg gtttgatatg gcgttccggt attatttgtg ccctcatgaa agcggtttcg
     1501 cccagcgctg tgatactgag tccgatgata gcaatttttt ttgaagcgct cctgctcgaa
     1561 tttgctgtga caatttttcg aaaaaatatt ttcgcatatc ttgttggcgg aatgcttgcc
     1621 atgtcgtgga cctttgtgca caaaattgcc gcatatctga ttatatacgg gttcaacctg
     1681 gtggaactgt acaggaatct ggcaatcttt gcccaaaaac aactgcacat tcaaatagaa
     1741 aacatttggt caccggtgat tgtgctttgg ttattgtacg gactgatggg tataattgca
     1801 gcaatacttg gaatttatat cggtaaaaag gcaattaaaa ctccgctcca aaacaattta
     1861 ttcagaggaa acaatgtata tcggaaccct gcacccaaac cggttcaagt atttaaacat
     1921 tcattgatgt ggttgattgt gaatggtatc agtatgatag gagtgctgct tttgatgaac
     1981 tatgctcact ggatatggtg gagcagcgca ggtgttgcca tagcatgtgt atgggctata
     2041 aaatacagga atgttttaca ccgtttgaaa aaaccgggct tctggttgtt ttttgttgct
     2101 attaccatga taagcagtat tctgttttcg aaattcagcg ccaatactgt aagttggatt
     2161 gatgggctga tgattggatt gcagatgaac gtgagggctg taataatggt catgggattt
     2221 gcagttatcg gcaccgaact gagtaatcct gttatcagga atttcttcgc cagaaccccg
     2281 atgcgacaag ttacaccagc acttgaaaca gcattcgaaa ctttacctta tgcaatatcg
     2341 tcgttacccg atattaaaat ggttttccgt aagccggttt caataatcca gcaaattgta
     2401 atgcaatcgg agtacttgct tgaaaaaatt aaattaaagc aatttggcaa agagaatgtt
     2461 gtaattatca caggcgaaat tcatcagggt aaaacatcta ttttacaagc attaatacct
     2521 atgtttaaag agcaaaacct tgttgtgggt ggaattattt caccgggtat tttcgaagat
     2581 aatatgcgca ttggttacga tataattgat gttgctaccg gagaaaggag ggtgttgtcg
     2641 cgaacgacag gcagcgaaaa tatgattcag ataggaaaat attttttaca cgacgaaggt
     2701 ttggtatttg gaacaacagc cctttcggtg gaaaataact ggaattcaaa tatgatagtt
     2761 gtcgatgaaa taggaccaat ggaactcgac aatcagggtt gggcgcaaag cgtgggagaa
     2821 ttgttacaag cctttgatgt gccgatgatt tttgctgtga gaaaaggtat cattgagcag
     2881 gtaactgaag aatgggctat tacagatcca ttgattattg atgctgaaaa atgcgatacg
     2941 acaatggcat tttctttaat ttctaatttt tttaaaaaca aaaaaatgaa taccgtatcc
     3001 gaacattatg tgaaggaatg ataattttat tatcaggtag tttaaatatt cgataagtac
     3061 tgtcaaggag tttatataga aatgagtgat aatttttcaa cgataatatt ggcatcggga
     3121 atgtccggaa gaatgggtga gccaaaagcc ttgctgaggt gggacagttc ttttactttt
     3181 ctcgaaaaaa ttgttgatgt atttgtaaga gccggcagca gcaacatagt ttgtgtagtg
     3241 aacaaggctg ctgagccata ttgtattggg cttaagttgc cttcatgcgt aagtattgca
     3301 acaaacaatc atcccgagtg ggacaggttt tattctgtaa aaacaggcat tcaacatgtg
     3361 aaggacacag actattgttt tatccataat gttgataatc cttttataac gcctgaaatt
     3421 gtaaaagaaa ttttcgcatc gagaatgccc ggggcatggt gttcaccggt ttttgaggga
     3481 aaaggggggc accccgttct tttatctaaa acgatatata gcagaattac caataaaatg
     3541 ccggacgaaa ccaagcttaa agattttcta ttcccatttc cccgcatcat tgttgaaacc
     3601 gacagcgatt gtattctgcg taatattaac actcccgacg attacaaaag gcttgttgcg
     3661 caacaataaa ctttcagttt attttttgtc ttttgtgttt tttacatcaa ttagttttgc
     3721 cagaatgctg atggctattt cggccggggc aatggcattg aatttaatgc cgattggcat
     3781 atcaacgcgg ttgagttctt cttcggtgag aatgttatct tgaaggaaag ctttccgcaa
     3841 tgtggcaatt ttcaccttac tgccaatcag acccagatag gccattgact tcttacccag
     3901 cacagcaaga atttcttcat ccatgtcatg gttgggtgta acaattacac agtaggtgct
     3961 gctgtcgggc tgaatttcgc ttgcgacttc aaggtagggt ttacacactt gcgtgtagtg
     4021 tatggcatcg ggattttcga aaatatcgct cctccagtcg ataagcgttg tttcaaaccc
     4081 aaactcgggg gcatatccgg ccaatacctt accgatatgt ccggctccga aaataaaaag
     4141 ttttgccggc ttcgctatcg gttcgatgta aacacacagt ttgccgccgc aagtcatcgc
     4201 caggtcggtt tgcaggttat attccctgaa tagggtcgat tgagttctga ttacttcgag
     4261 cgcatcggca atcacctgct tttcgattcc accaccaccc acagttcctt cgattgcccc
     4321 atcggaaaac accaccattt tggcaccggc ttttccgggc gtagatccgc tcattgaggt
     4381 aacaatgcac aacgctgatg cacgagtatc tttgtaataa tccgaaatct ttaaaagtaa
     4441 gtttttcatg tatagtgaat tataagtaat acaaatctaa aaaattaaac tcaaattcag
     4501 gatgaaaaaa caaacagtat catgcgctaa tgtgattcag ggctcagatg aacagagagc
     4561 aacaacacct atttgcttat cggatcaatg cagaactctt ttcgcttgca tttggagcaa
     4621 tgtgctccca tccataccct gtcgaactga tttacagctg catctaccaa ttcagggtcg
     4681 ctggtaagga tgccggactc gaaattccgg ttatcgctgc ttttcatgcc tagacctgca
     4741 ccggtaaggt tggcagaacc tatataagcc gttttattgt cgaatagaat cagcttacaa
     4801 tgtacccttg ggcacagcat ccgttcaaga ccctcgataa gggcagggta gcggtcgaaa
     4861 tcttccctga aagtgcgtcc tggttccgaa gcatgaagca aacgcaccga cacgcctctt
     4921 gtaataagat ctgaaagcaa ctcaagcagg ggtacaacct ttgtaccttt ctttacatac
     4981 aaatccttga tatcggctgt tgcaatccaa atgctacgtt taaccgcagt gaataattcc
     5041 acaacttcgg tataatgttg ttcgttttgt ataaaccgtg tctggctcat ttcaacgata
     5101 gtttgctctg gagtttcatc gattgccgca tcccacgcaa atacacacga ggatcggttt
     5161 ttttataggg tacaatcata ttcagcgaac ggggtactat cgaaatttgc acccgtttgc
     5221 atctgctgga aggctctccg tcgaactggc agggcacctc gttttcagtt tcaacaattg
     5281 cactgtcggt tttatatatc tcaacgtatt gagaacggtt gatgattttt aggaatagca
     5341 tgttcgcaag atgcgggact ttatacatgg gtataggatg aacaatgcaa agatcgatca
     5401 ggccgtcgct gatgcgggca accggagcaa tgcttgcgtt aaacccgaac tggtcggagt
     5461 tggcaaaact aacaaacaat gcttttgttt taatgtcttt atctttaaat cggatgtggt
     5521 ataattcggg tttatagctg aaaatgttcc ttttaataat ccttaaatag ggaacaaacc
     5581 cacgttgttt gctacctgca aattcttcag ccaccaaggc atcgaacccc ataccggcaa
     5641 tactgataaa catggtatcg ttcagcaaag cagtatcaat acgcttaata ttatgattat
     5701 taagtatctc tatcgatttt cggtagataa caggtattct taaatagtgc gccagaccgt
     5761 tgcctgaccc aacagggata agacccataa ccgtgttgct gtttaccagc ccacgtgcaa
     5821 cttcgtttgc cgatccatca ccgccgactg caaccacaac atcggtaccc ttttccgatt
     5881 cgttgcgtgc tatttctatg gcatgatgcc tgtaggcagt atagattatc tgataatcgt
     5941 acaggtcacg atcgagatac tttttaatgg cagcctcaac cctggttttg cttcgccccc
     6001 ccgaaaccgg gttaatgata aaaactattt ttttctttgt ttcaggcatg attttgcaaa
     6061 ggtaatcctt tcagcttata atggttcgat tggagtttgt gagttattaa cggacatcaa
     6121 tattgtttat ttttatattt ttgaaaaaaa accgtaacga tgaacaaact gcttatatca
     6181 ggttttatta ttgctatggt atgcaatact ttgaacgcac agcaaagctt attgggcgac
     6241 aacccgatac atatcagctt tagctactgc aataccgata atgtcgaaaa actgttgcgc
     6301 aagaataaaa caatcagtta cagcgatctg cagattcagg agcttcgcat ggagatttgg
     6361 aatcagaatg cccgcatcga caaggaacag caggagctga taaaccgaag gaatgaattg
     6421 atttcggtgc ggcaacagat tgtccagctt ggaaaccccg aactgataac ggcaaggata
     6481 aatgaaaaaa aatcaagtat cgacaaaata aatgctgata tccgaaacaa cctgtcgggg
     6541 ttgggttata aaggtcttta tgtgtttgcc tgtaaaattt cgtcgagtta cgacaccgaa
     6601 aagcagttgc gcgatcaggc tatgaaattt attatcccgg ttgccgtaga aaaaacaaat
     6661 ggcagttata ttgcctcggt aactgagttg cgcaggaagg aaggctacga cgatactctg
     6721 taccgttata tcagcgaggt tacccagggc acttcgaacg tggaggcaga aatcatcaac
     6781 cgaatgaatc gcaaaggcgg ttactatatt tatatggcaa aactaaacac aaaacctttg
     6841 cagaaagaag ttaaaaccct gactgctgcc gacggcaaca atccggaata tgtggtggta
     6901 ttcgatgccc tgcgcgataa aaatatgacg aaaaaaattc ttgaagtcgg tgcatcaaaa
     6961 gacgatctga aaaaactgga gcaggagatc gataacaata aagccctagt attagccgaa
     7021 aatgccagga tcgaagaaat tgaacggaat tatattctat cgggcaacga acgaataaac
     7081 caaaccctgc gcgaaataga gaagctcaat gcagagtaca aaagcaacat tgaagcactg
     7141 caatcattga tgcaggtgta tactcccaat ataaagctcg atgaaaaatc gccaaagaat
     7201 agcataaccg cagttataaa ggagatcgat gataaaatcg ccgcgctcga cagcggaatt
     7261 atggagaagg agtcgcagcg gctctatggt gatttccaga ttctggtgac ttcgggcagt
     7321 gattactttc gtaaaattgc cgaagaaagc attgcctcaa aagaccgttt gaccgagatg
     7381 tacgcaaaag ccaacaatta tgtgcaattt gtggaaatgg aaaacgacga ttaccgcaaa
     7441 gcagtgggta acgaggaggt gtatcagcgg tatttcgaaa atatgtgggt atttcccgag
     7501 ccgggtgatg gcgatttttt cagggtaacg gttattgtta agttttcgct tcagaaaacc
     7561 aaacctgcct ggaaagtctg gtaattttct gataggtttt aatctgcagt ttcttaatca
     7621 tctgataaaa aaatccagca ctgaatcgga gatatatttt agggtagaat catccatttc
     7681 ggtatgtatc ggtagcgaaa gcactgtctc acacaagcgg tcggtaacag gataatcatc
     7741 agcacaatag ctgtagccct gaaaagcatc ctgtttgtga aggggtacag ggtaatacac
     7801 tgccgaagca ataccttttt ccctgagatg attttgcagg gcagccctgt cggtatcttt
     7861 gagtatcagc gtatactgat gaaaagtatg cagcgaatag gtatttcttg cgggtacaat
     7921 aaggtttttg cacgatgaaa atgctttagc ataataatct gcaacgcgga tacgcgcctt
     7981 gatatagttg tcgagatgtg gaagttttgc ccgcagcact gccgcctgaa tgctgtcgag
     8041 tcttgagttg accccaattc tttcgtgata gtaacgaacc cgcatcccat ggtttgtaat
     8101 gctttgaata agataagcca ggttgtcgtc gttggtgtat aaggcgccac cgtcgccata
     8161 gcaacccaga tttttggaag ggaagaacga agtgcatcca atgtgcccga tggttcctgc
     8221 cattgcagaa ttgccgttgc tgaacaggta gtggctgccg agtgcctgag cggtatcttc
     8281 gataacaaat aaattatgct ccccggcaat tttcatgatt tcttccatat tggcgcactg
     8341 cccgaacaga tggacaggaa caatggcttt tgtgcgggga gtgatggctt ttttcaattt
     8401 ttcggtatca atattaaaat actgagggtc aacatctacc agcaccgggg ttaacccaag
     8461 cagtgcaata acttcgacgg tagcaataaa tgtaaaatca gcagtaatca cttcatcgcc
     8521 gggttttagc ccgagagcca tcatcgcaat ctgcagtgca tcagtaccat tagcgcacgc
     8581 tacaacgtgt tttaccccga ggtaagcagc cagttcctgc tggaattgct tcactgcatt
     8641 gcccccgata aaagcagtat tatcaataac ctcgtgtatg ccattgtcaa tctcatcttt
     8701 tattttcagg tattgactct tcaggtcaac catcggaatt tctttcataa ttgcaatttt
     8761 gttgctaaaa taaaactttg catggtatga aaaccatatt caacgaaatt tatgtaaatt
     8821 tgcctcataa ttgtgtagaa atgaacctgc aaaactatct tatttcgttg ctgtatgagc
     8881 acaactgtgt gatattaccc ggtttcggag gttttgtggt taattacaag gaggcggtaa
     8941 ttgattttca gaatcagaag ctttctccac catcacgcat cgttgctttc aaccaaaacc
     9001 taaatgtgaa cgatggttta ctcgccgatt ttattgcacg cagcgagaaa attacatacg
     9061 atgaggcgtt gcataaaatc gaaaactttg ttgccgcaat gactgatgat ttgggcagga
     9121 tgccgtctgt agctctttcg ggcataggca gttttgcgat gaaccacgat acgctagaat
     9181 ttcgtccgga agccgggcag aatttcctaa gagattcatt cggattggag agctttcatt
     9241 tcccattgct gaaaactgcg aaaccacgtg aagttatcga agaaaaagca aaaacagttg
     9301 tttccacggt tactgtaaac cgacgcatta aacccagttg ggcaatcgga attccggctg
     9361 ctgcagcaat atttatactg gcggttatgt ttttcaatca ggataacctt aagttcaata
     9421 ttcagcagaa taccgaaatg aatccattgt atgtgggtcc aacaaaaaat attgacgata
     9481 aaacgaatac tgtttcagcc gaggctgttc tgcctcaggc ggagaagcct gatgcaacat
     9541 ataatcaacc cgctgaagtt caatcggtag atgttgcggc acaaaacgaa cttaaggttc
     9601 atgttattgc cggaagtttt tcgcagcagg aaaatgcgct aactatggct gaaagtctga
     9661 agacagttgg atataatgct cagatattgc atattgaacc atggtataag gtaagcatta
     9721 aggcgtttgc cactcccgaa gaagctgcag cagaactcaa tgcattgaaa caggcaaccg
     9781 gcaataatgc attgtgggta atgactaatt agtcgttgct aacgacatca acctaaaaac
     9841 aacgtagtga cagaattcat cattttaggt gatacttttt tgtgaaattg ctgcaaatga
     9901 aatgtaatta tcgataagtt ttgcgtaaga tgtgcaacca ttttcggttt catagtcttc
     9961 tatatgaagt atgatgagcg atcaggaaat tattaagggc tgtattaaaa gccattccga
    10021 tgctcaggct gcattgtata accgctatgc ccctgtgctg agaggtattt gtatgcgata
    10081 caccggaaat aaggacgatg ctgaagatgt gcttcacgat agtctgataa aagcgctaat
    10141 ggcaatgaaa caatacaagg gaaacggatc gttcgaaggt tggctgaaac gtatcgctat
    10201 gaatacctgt ataagttata tcaatggaaa gaacaaatca ggagtatcct ctgtgaacag
    10261 agtggttgaa aacgaagcag atgatccgga agaaactgaa ggaaatcagg gtcttattga
    10321 acgtttcctt aacagcggga tcgaaaaaag cgatttattg caggcggttg ccggtttacc
    10381 cgatgggtac cgtatggtgt tgaacatgta cgtgattgat ggcatgccgc accatgaaat
    10441 tgctgctcaa cttggtatta gcatcaatac ttcgaaatcg cagctctctc gtgcacgaaa
    10501 tctgcttaaa tcgagattgc ttggtatgat acaaaataat gaatttaatg tacatgtaaa
    10561 tgaaaaatgc tattgacaac aaactcgaca atgtactacg agaggaactg ggagcaattg
    10621 aaatagccgg tcctcctgcc ggacttacgt cgtttatttc gaaaatcccc aaaacagggt
    10681 tgtcgtatgt tacccagata attataatta ctgttacctc tatggcagta gtatttacgg
    10741 ggattatcat gtggcaggca ggagcaaacc atgcttatca gaaacttact gcaaaagaac
    10801 ttcataatgt ttctgaaaaa caattattcc gtgtcaatta tacctcgaaa ggtgctttgc
    10861 ctttggaaga tagcaatgaa cgcttagctt ttgcaacaaa caatacagtc cagaacagac
    10921 atactggtgc agcgattagt acgactaaaa atgatactta cataattaat gaatcagaag
    10981 caaatgccag gactattatg cctgcgggta ccgaaaacaa tgactttaaa aaagatgttt
    11041 cggctccgta tttttgtgat actgtggttg tttacgatac ggttgttgtt gtcgataccg
    11101 tattaaaatt ccgtcagcaa aagattattg ttaaatcgaa acgtaaaaat actcgccggt
    11161 aaatgaattc acaaagtaaa tacttgtact ttattgcact gttgatttta ttattaccag
    11221 ccggagaatt gttttctcag caaactgata cggtaaaagt acagataaga aaggtggttt
    11281 acgatacagt atttactgaa gtagaaattc cggtatatga taccgtttat aaagaaagat
    11341 ttataagcag tctttcgttt tcaccggcca taggagcagg agtgggggta aatcgttttg
    11401 gaaaggacac agatagtact gcattgaatt ttctgaaccg gacctatgct gaatcatggt
    11461 tttacagtgt cggtactggt tttacagcca ccagcggtcg ctttggaatt ggtacagggg
    11521 tatattacac tgcaatggat cagaaattta tctatagtca ttcaactttc aactatgata
    11581 ctataaccta ctataatacg gaaatacacc tcgaagtgca aacagatact gtatggttcg
    11641 actcgataaa ctatcaaata actacgattt cgaattatac ccttgattct gtaccctacg
    11701 acagtgtttt ggtgcatacc catgatacaa gcctgaatta taccaacaga tacagaatga
    11761 taaggattcc gcttctgctg agttattctc ttatcgataa gaaaggtttt gtgcttaatt
    11821 tttctatcgg accggtgttg ggaattatgt tccgtaatga ttcgtacagt cttggccttg
    11881 atttgtaccc tgtaaaaaat gaaaaaaaga tatattttat cccatatatt tacggagccg
    11941 ttggtcttga gtacgctttt tgtccgggca tttcgcttgg ttgcgatctg ttctgttgtt
    12001 ttcctctgaa aagcattggt aatagctctg gggtttattg gtataatatt gataattatg
    12061 gagttgcatt gaagtatttg tattattttt gaaatgctga tttttgcgat gcaacctttt
    12121 cttatctgta agtcttatta ttgaaaaaca atatcaacaa tatgaaaaaa ataattcttt
    12181 tctctctgct aatttcaagt tttatcatcc tgccacaaat gctccgttca cagaattgct
    12241 atttacaggc aagctacgaa gcaaatggtc tggatgtaaa ctttgccgca caactctacg
    12301 atttaaccgt caatcagcag gacagctctt ttcaacccat ccaaggccag tatatttggg
    12361 taatcgacaa tgcaacattg tcgggtcaga atcttgagtt ttcttacaac tcctacgggc
    12421 agcatgttgt taccgttacc ggaagcggga tgggttgcag tgctactact acgcttattg
    12481 taactctcga cgaaaataac ccgatcgaca gtactgggaa ttttgtggtt cttatttccg
    12541 gcgatagcta ctcatcactc gattgcgatg ccgatatcaa cgcatatgta attggcggta
    12601 tagcccctgt tagttttaac tggaataatg gagttacttc aagtttactt aacgatgtgt
    12661 gcgccggtga gttttgcgtt accgctgttg atgcaaatgg aacaaccgct gaatcctgtt
    12721 tcactgtagg ttatggcaac accgatacat tgccaaacga ctcgaccaat tatggaaaca
    12781 tttatgtaag cattgagtac tattcaaatt cgttagatga ttgtggatat tcagcatggg
    12841 caagcgtgta cggaggtact ccgccatatt tttatcaatg gaataccggt caaaatacct
    12901 ctcaggttgc cgaattatgc gtagggcagg aaatatgcct tatggtggta gatatcgatg
    12961 gcaattcagc tactgcctgt gcattgattc tcgccgacag tgtttataac accgataccg
    13021 tattgaatgg cggagatcca tgggaatcga cttttgaatg gcagggcgat tcattgtttg
    13081 ccgatgctca gattgttcat tactggatca ccaataccga aatatgtttt gtctgggctc
    13141 ttatctacga aaattcttat caggaaacca tgaccgtttg ctatcccctg tccgacagtt
    13201 tgaatagcgg ctggtacgaa gtaatactgc atgttatttt caataacgga caaaaggcaa
    13261 atatcagttt tattgacaat gtgtatctag aaccatccga cttatcgtcg aaccctgata
    13321 atgaaaacat ccttgatgtt actgcatacc ccaatccggt tggcgatatg ctcaatattt
    13381 cagtttcgaa gcagggaaac tcttgttcca tcagtgtgtt cgatcttagt ggcaaactca
    13441 tatataaatc agactattca ggcaacgagt tgatacgcat cgaaacgtca aattgggaaa
    13501 atgggatgta ttttcttgaa gtacgtagtg ataatgaaaa gtcagtgctt aaaatcgtga
    13561 aatagagtac tgttttaggt aaataaaaaa tgcctggctt gttgagtcag gcatttttat
    13621 ttgaatgaat gcttattttt taataatggc tttcattact ttttctttat cgagtttttt
    13681 agtgcagaag aaccagtcat agcattttac atcttcatct ttgcttagca ttctttcttt
    13741 cgctacacca cacgatccgg cagtcggaac aattacatcg tcgcccggac gccagtcggc
    13801 gggggttgct acgctgaatt catcggctgt tttaagtgcc agaacaacgc ggtacaattc
    13861 atcgaaattc cttcctagac ttagcggata ataaattatt gtgcggataa ttccttttgg
    13921 gtcaattaca aatactgctc tcactgcttt ggtgttgctt tcgccaggca tcatcatgcc
    13981 atatttggtg gcaacttcca tagtgatatc ctcaattaaa gggaatgtaa cctcaacgtc
    14041 tttcattcct ttatactcta ttttttcctt tatggtacgc aaccatgcga tatggctgta
    14101 aagaccgtca accgaaagcc ctaccagctt gcagtttgcc tcattgaatt tcttttccat
    14161 cgaggcaaag gtcataaact ctgatgtaca cactggtgtg aagtcggcag ggtgactgaa
    14221 caatataacc cagcttcctt tgtaatcacc gggaaaacta attttaccct gtgtggtaac
    14281 agcttcaaat tctggtgcac gatcaccaat gcgtggcatt gcatttactt tgttttcttc
    14341 cattgttata attttaaggt aatggttatt tatttgtaat tagtaacaaa tgaataatgg
    14401 aaatgttcag aaaaatgtgg acttaaaaaa tgagaaatcc cggtaaataa agaccagaat
    14461 ccttattgta caactattct gccctgatag gtataatctt gtgagtatag atggtatata
    14521 tacattcctt ccgaaatatg gccgatgtcg atgtggtgat cctccggtaa aaattgttgg
    14581 aggagcaatt tcccctgtac atcgtagagt tcgatttgta ttggctgggc catatttccg
    14641 gtgcggaatt caataaaatt gtcagcaggg tttggaaata ccgaaatatt ctctgttgct
    14701 ccgatagggt tgcccacgaa ctgagtagag taattcaggc tgaatccccc tgcgcgggta
    14761 ttcccgttgg ctttaaaatt cactgtaatt ttatacgctt caatggtaag aacatcaggt
    14821 agggtactcc ccgagtaggt agcaactgtt tgcgaagact gattgagtat ttttacataa
    14881 tcataaacag gctccagatc gaaatcggtg aattcaaccg tgatgctttg ggcgttttcg
    14941 ggttcaatat accagtagca ggttgtatta ttctgataat tatactggtt gctgccgtca
    15001 gaaacggttc ctaccggttc cgaaatatgg gttaccagcg gacagtacac aggcttaata
    15061 cctttgtacg ataggagaaa cccgtcacca ttcaggtcat tgtccgtaac aaaacgaatc
    15121 agcacctcat cggaagtgct ttcaaatgtt gcagggattg tgttccctga aaaaactccc
    15181 aacacctgag cctcatcggt aggtccatcg aaaatggtta taaaatcgtt agggtcggtt
    15241 tgcagtttta aaaattcaaa ttcaattcca ttggccgagt cgtttggttg aatgagccat
    15301 tcgcattccg taccggcttc gtaatcgaaa agaggtccgc tgccgtcatc gatggtacct
    15361 gctgtagttg tcagaatttt ttgggattga cagccctgcg gatagatacc cgaaggtact
    15421 gcgttcacaa taacttcgtg cagcaaagtg aagtcgcttc caccgggtgt aaggttgtcg
    15481 aggtagaaat acccgtcgta acttccgccc caaccccaat tgaaatggta ataatcttcg
    15541 gtttggtagc cgtcaacaat aaatgcatga cccgatacaa actcgtaatc gctccaaccg
    15601 gcataataaa gcgggattct ctgatcgagg tgctgaatta atgtgccttt ccagtcgaaa
    15661 tcttccggaa gactgtcgcg gaacagatag gtggtttcat catcgtaacc gaagtaggtg
    15721 tggagtgtgt aggctccttt gtgattccac attcccgatc catcgggacc gtaattcata
    15781 tccacagaaa cgccgatgtt attaattaac cgtgccacat catcgttgaa atcggtaagt
    15841 tctaccgcca tctgatcgta attatagttt tgttgcgaaa agtcaacttc aatccatcca
    15901 taatcgtcgt gctgatatcc ataagaaccg gttccctgtt caggatatct gaaataattc
    15961 aacaattgcc ccaatgcagt tgcaacacag ccggtaacaa catgaccacc cggtcctgca
    16021 ccgtcagcag ggcacatggc attgtaatac aacccctgat tccattgggt aaacaacata
    16081 ggcgtcaccc ctgttttcac cgatttaact agactactgc tttctccctg caaatgcgac
    16141 cacattgcag caatattttc atcaaccaat gtttgattat caatcacatg ggcaatttgt
    16201 actgcataat tatcgagcca tgtagcgcag gaagaattca tttcagcggg aacttgggct
    16261 tctgcaaatg aataacccag caccggaacc accttttcac tttccgaaac aattacaaat
    16321 cctgaaggat tcattgcaca aattgtatat acagctttgc cgttatgcat ccgcgtttcg
    16381 gttgatacaa cactgatatc atcgtaatga acacttttaa aagcatttgc ctgctcataa
    16441 tagaaatttc tggctacaat ttcagttctt ttattttcgt tggattgtgc aaacaaaact
    16501 gcagcaaata ataacaatgc aagggtaata tatactctca tagcttatac ttcatttata
    16561 tcgtttaaaa aatcagcaat tttttgagaa cgaagcatgc gttcgtgcag aagaatacgg
    16621 tacgattcga tattatcgat attcttgcgg gcaactcctg ttttcattgc tgccatggca
    16681 accgcaggtg caacatactc aattactctt ctgtcgagag gtttcgggat aatgtaattg
    16741 atgccaaaac tcagttcttt tataccatag gcttgcatca cttcttccgg aaccggaagt
    16801 ctcgccagct cagccagagc tcttgctgca gccattttca tttcctcatt aatgtcgctt
    16861 gcctgtacgt cgagggcacc tctgaaaatg aacggaaatc ccaatacatt gttaacctga
    16921 ttcggaaagt cggagcgacc tgtagccatg atgatatcgc ttctggtttt caatgccaga
    16981 tcgtacgata tttcgggaac agggttggca agagcaaaaa caatcggttt cgcattcatc
    17041 gaaagaagca tgtcggggct tacaatattt ccggcagaca ctccgacaaa tacatcggca
    17101 tcttgcatac actcggcaag ggtattcaaa ttccgatgcg taacaaagca tttccgctct
    17161 tcatcgagat ttgttctcga ttggttgatt accccttttg tatcggtaat aacaatgttt
    17221 tcatgaagaa cccccagttg ttcgaaaaat tttgcacatg aaatacctgc agctccggca
    17281 ccactaaaca caactcggat ttcctcaatt tttttaccga ctatttccag agcatttaac
    17341 agtgcggctc ccgaaatgat ggcagtacca tgctggtcgt catgaaaaac agggatatcg
    17401 catatttttt tcaattcacg ctcgattctg aaacattccg gagctttaat gtcttcgagg
    17461 ttaatgcccc cgaatgtagg acagagtgat ttaacgatgt gaataagtgc atcagggttg
    17521 ggttcatcaa tttcaagatc gaaaacatcg atatcggcaa aagccttgaa aagcagccct
    17581 ttcccttcca ttacgggttt tgatgcatgg ggacctatat ttccgagacc cagtacggca
    17641 gttccatttg aaattactgc aacaaggttc gatttggctg tgtaatcaaa tacggtgcta
    17701 acatcatctt taatagccag acatggttca gcaactcccg gagtgtaagc caacgaaagg
    17761 tctttctggg ttatataggg ttttgttgca acaacttcaa tttttccagg cctgccattt
    17821 ttatggtaat caagcgcatc tttgggtgta aataatgtca ttataaatcg ttttcatcgg
    17881 tgggtacatc gtcgtcgctt ccaataaagt ctctcgcatg cccatggtct ttcatggcgt
    17941 ttttcatgag ctctttattc tgttgtcggt ttttaaatat atcaatagcg aggtacagcg
    18001 cattgcggaa cgattcttcc gaagcttgtc cggtgcctac cagttcatat gctgtaccat
    18061 gagccggaga tgttctgaca ataggaagtc cggctgtgaa attaactcct gtgtcgaatg
    18121 caagggcttt gaagggagcg agtccctgat cgtgatacat tgccagtaca ccatcgaact
    18181 tggtgaaatt gtccgaccca aaaaatccat cggctgggta ggggcctaaa gccataattt
    18241 tatggtttcg tgcttcttca atagcgtgag caataatatc tgattcctct gtccctatta
    18301 cacccatatc gccgcagtgc ggatttagtc caagcacagc aatacggggt ttgcgtacgt
    18361 tgaagtcgaa tttaagcgat tcgttaaata ttttcagttt cgaaacaatt ttttctttgg
    18421 tcagtgtcgt ggcaatgtct ttcacaggta tgtgcccggt agcaaccccc actctgagtt
    18481 tgcccgagac cagcagcatg aggtgttcct ttgtttcaaa tttttcggca agatactcag
    18541 tgtgtccggg gaaatgaaac ccactttcct gaatattgtg cttgttgata gggcctgtga
    18601 ccaatacgtc gattttaccc tgctgcatac tttttactgc gcattcaagc gaagcatacg
    18661 atgcagcccc agcttcggga gtcgatttac caagttcaac cttcacctcc tcgtcgatac
    18721 agttaataat gtttgatcgc ttggggtgtg cttcttcagc tgaacgtacc tgattcagac
    18781 tgaaattttc gatgtttaat gcttttcggt gataggcaac tacttttgga gacccaaaca
    18841 aaataggtac gcacatatcg ttgattcgtg tatcgctgag tgttttaata attacctcgt
    18901 aactaatact gttaatgtcg ccatgactga ttccaaccct gattttatcc ataattacga
    18961 cttaagattt atgtaaaaac tatattgtaa aattttatgc aatattacac ttattagtga
    19021 aaaaatcgaa cagaagccta actccgtttc ctgttccggc tttccctctg tatttatcag
    19081 gactgtcgag atacgccgtc cctgcaatat caagatgagc ccaggggtaa tcggtgaaat
    19141 gttccagaaa tttgcctgct gtaattgaac ctgcccattt gccacctaca tttttcagat
    19201 cagcaaaatc agatttaata tattcgtcgt attcttccca aagcggaaac tcaataatgc
    19261 gttcgtaggt ttcgtttcct gatgccacta attttttcag tagttctttg ttgttaccca
    19321 ttcctgctat tgcgatggtt ccaacagcca caaccgcagc gcccgtaagt gtcgccagat
    19381 caattacagc ttctgggtca tatttttttg cataagccaa ggcgtcagcg agtaataacc
    19441 gcccttcggc atcggtattc aaaacctcaa ctgtagtacc attgaacatg gtaataatgt
    19501 cttgagggac ataggcattt tcaccgggac gattatcggt agcaggaacc agtgcgatga
    19561 cataaacagg taacttggct ctggcaatgg cattcataca accggttaca gcggctgctc
    19621 cggccatatc gcttttcata ctgtccatac ttccctgagt gggtttcagg cttaatccac
    19681 cggtatcgaa tacaatccct ttaccaacaa ttacataagg ttttgagttt attgcatttt
    19741 cgggtttcca ggtcattacg ctgaaactgg gtggttcttt acttccgcgg ttaacggcta
    19801 aaattcctcc cattcgcaga gtttcaattt ttttcttatc gaaaacttcc actgaaaaac
    19861 ctgcatcttt acccgttgcc tgaatgcatt cagcaagctt atgcgctgtg aggtacgaca
    19921 acggttcgtt cacgaggtcg cgggcaatgt acaccgattc tgcaagaatg gtaatcattt
    19981 caatgctttt gtcgctgata ttttctgatt tgatgtttat ctgctccagt gcttgttttt
    20041 tcttatcctt atcggtaaaa tattttgtga aagtgtaggc tgaaagaacc aacccctctg
    20101 caaatgcaac cgcaaatgaa agctctctgg tgaaatcaac aatatgtatc aggttgattt
    20161 tgtacttatt tatcagattg cacacctcat atcctttaat acgcaaagcc tcttttacag
    20221 tataagcttc tcccccggat gaaggcgcaa ccacaataaa gctccactta aaataggaat
    20281 ttatacagat aagtgtttcc ttgctttcga gctgcgattt cacataggca agctctgttt
    20341 ttgtaaaaga aagtgcattg caatcgaagt tttctggaat caggtacact accggatcgt
    20401 cgacgggtac tgtgcttact gatttgataa gaggtatgtt gcgtttcata tgtaattagt
    20461 taacgtagtc ctgtcgtttt acaatggtat aaaaatcatt ttcgttttga atatatccct
    20521 gatcgtaaca gaaaaaatag gtgtttcctg tttttgtagt gaacacattg gtaaggtaat
    20581 cgaaattaaa gccttcatcc atgaggcgtt gccgcgtaac acgggttttg ccctttttgt
    20641 tgagcgattc tagtatcctg cggttttttt tcaatatccg gttgatattg cgcatcatat
    20701 tggttgaatc ctgattgagc ctgttgttat aagtattgcg gcattggtcg ctgcaaaact
    20761 ttttatcgac acgccctgta agtttttcac cacattcaag acagcctgtt ttcatagtct
    20821 gatgatttgt taccaaagat agtaagaatg gatgaattgc acagcgaaaa tggcgaaaac
    20881 tttcatttct tcatggtcta aaccgatttt cagaagatgt ttttctacct gttaacagta
    20941 aatatccggt attttgccac tctgattttt tgcaaaaaat attgcagcga tgtttttagc
    21001 actcccatgc catacttaat acttcgccgg aaattaattg agctggcttc ttcgaaatac
    21061 agggtagggc aggtaatttc ggcaatttcg aatcctgcaa agcaaatctg cgcaagcatc
    21121 tgattatcga aaacaaaatc atcagaatta aggttataac ttattttgcg aagcacgtca
    21181 gatgaaaacg cccggtatcc ggtgtgatat tccgaaagtt tctgatgcat gagcaaattt
    21241 tgcgtaaaag taagcagtcg gttggcaatg tatttataca agggcattcc tccacgaagt
    21301 gcacccctgc caagaattct cgatcctaaa actacctgat acactccgtt tgcaataagg
    21361 tatgccatag aatgaattaa acggggagtg tactggtaat cgggatgaag cataatcaca
    21421 atgtcggcat tcatttcgag tgctttatta tagcacgatt tctgatttcc tccataacct
    21481 gtgttatgct cgtgaatgat aatgttcctt attcccagct gccgggcaat acctacggta
    21541 ttatccctgc ttgaatcgtc aaccagaata acatcgtcca caatatcgaa agggatctcg
    21601 gaataggtct gagccagtgt gctttctgca ttataggcag gcagcactat tacgagtttt
    21661 ttgccgttaa tcatttattt gtctgctgtt ttaattgatt cgactttccg atatattcat
    21721 tggcttttgc catgttgccc atctgatagt aagttactcc agcattatac cagctttcgg
    21781 catcggaagg gttgagttcg attgatttga gaaagttttc gagtgccgaa ggcaggttgc
    21841 cctttatgcc ataggctact cccagatcca tgtaaagacc tgcgttcggg tttttcaatg
    21901 aagctgattt tgaaaggtaa taaatagccg tgtcgatatt ctgaaggtat ttgccataga
    21961 tagtaccctc gaggtgatac agttcaggaa ttcccggctt tattttgtcg agtttttgga
    22021 tggcatccag tatttcttca ggcgtgtttg acgatttgcc cgcattgagt aaaccatacg
    22081 agttcataat gatgatttta gcattattaa gtgcaatgcc atgatagggt ttcagtgaaa
    22141 gtacttttgt gtagcaaccc aaagctttta ccgagttggt agtgtattca taccatgcat
    22201 tccccaacag gttgatagca tcaacgtact cgggatatat ttgtatcgat tgctccagat
    22261 attttatcgc atcacggcaa tattgattat gaattgcagt gtcttttttt atctccgatt
    22321 tctgggcttg ttcaattaat ttacctccag cactgcatgt gcttttggca gaattgtagg
    22381 aagttttcac atcagtggtg aataatacaa aatcgttttc ccatgcttta tttcgcgaaa
    22441 aggtctttcc cgaatataac accaaaagaa tgcaaagcaa agggatgctt agtgaagtgg
    22501 ctttcatctt gagtgtttcg ctcaactgta acaatggtcc ggtaattatc caacccatca
    22561 aaatgcagaa tcctatagag gaaataaata tgaatcgttc gttcatgaaa gtgccgaccg
    22621 gaaagaaaat attggaaaca accgataatg gaataatata gaaccagata ctgtacgaaa
    22681 ttaagtcacg tttcttaatc ataccccata ttgcataaaa gcccagtgcc agatatgcaa
    22741 gcagagtaac aacagggcgc cagtcgttcc atccgacaat tgggatttgc tttgggtaat
    22801 agtcataggt taagggatgc ggaaaaaaca aaagctgcag gtatttaccg agagtaagga
    22861 aaatagttgc aaatttttcg ccaacagagg catacaggaa cgggttgttc atgagttcct
    22921 ctgcaatttc tttttcacct cgtcctccgc cgagaatact ataacgtatg agcagaaata
    22981 ctccggcagc aatcaacaga ggcacataag cacccagcgt ttttacaaac ttgtgtttcg
    23041 aaaaataata cacggtaagc ggtattactg ccaaaaaggt aattgcattc tcttttgaaa
    23101 gcaaacccaa aaaaagcgat acccctgaaa atatcaggtt aataaatttt ctagtatcaa
    23161 ggtattttaa cgtgaaccat aaagccaaaa gtgatccgag caatgtgagt atttcatcac
    23221 ggcctttaat atttgctacc gcttcggtgt gtatcggatg ggcaaggaaa agtaccgata
    23281 ccacaaaagg cagcgaaata taccaccggg tctcaggttt ggggggtatc agcctgacta
    23341 gtattttata cagcagcagg gtggtgagaa tgtagaggat aatgttgttg aaatgactaa
    23401 tataaggcct tccggttatt ttaggatttt cccctttcag tgtgctttta tcaccaaaaa
    23461 cctgcagttc catggcaaac gtcgcaagag aaagtggccg ataccgacct ccggctacca
    23521 gttttttatc ttcctggatt ttttcggcag aaacacccgg ataacttgtc atccaaaagc
    23581 cagtaaatgt atcgtaccgg aaaatgtcgg gaatcccggc aatccctttt gacgtgaact
    23641 gattgtcata tattacaatg gcatcatcaa gggcgtagtc atgtgtcagt gtattggcat
    23701 acagcatagc cccaagtact atgattacaa tcatcgcata aggattggct ttcacggcgg
    23761 gtatgggtag aggcttccca acttttttag gatcagtgat aatcttcttt tttatgggct
    23821 gctgttttgc cacgacaatt tgttttgtca aaaataaaaa aaagcttcga aattgacata
    23881 acaaaaaagc acatgctaat tctggatgaa atgcacttga tgcaaatagt tttttttata
    23941 tattgcctca aattgtaaaa caatagcaat aagaaaacag ttatagttaa aatgccgaca
    24001 gctatgaata aaaaaatatc gttagtatta ttatggatag caacaattct gccagctatc
    24061 gcacagcaaa acatcgcggt tttgggggaa attgatgttt tacaaaaagg agaccctgta
    24121 tcattgcttc gtgttgatgg tagtgatgaa acagttgctg gggcagtgta tagtgatacg
    24181 aactttgaaa tgaattttca ggcttcacag tccagtttat atatgtttta ttatggtgat
    24241 gaagatggtt ttttaattgt attacatccc ggcgatactt tgaagatagg tattaataaa
    24301 aatgaaatcg tcatggaagg ctcttatgaa aataagatga attcatttgt aatgatggaa
    24361 agcaatttat taaatgaaaa atataacgag ctctacgaag agtatcttag cactgaagat
    24421 attgataaac aggtaaaaat caatgaagaa atcgataccc ttgttgcgcg gtataatgcg
    24481 ctgattaaaa gttttgttcg aaatcacccc ggatcattgc taagtttgat atatcttgaa
    24541 gaattgaata ttgatgagga ttttgagttc ttcttaagca gctgcgatag cttgatgatt
    24601 aa
//
LOCUS       DAOOQP010000032        24092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2546_length_24092_cov_5.703832, whole genome shotgun sequence.
ACCESSION   DAOOQP010000032 DAOOQP010000000
VERSION     DAOOQP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_24092_cov_5.703832"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1306
                     /locus_tag="PLK75_05230"
     CDS             260..1306
                     /locus_tag="PLK75_05230"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY38684.1"
                     /translation="MKTILAFSTVIALTLLCRSVYAQLVTTTNVDPAQTVSEFILNGV
                     EASNVVYTGDVNAFGTFTGGYDTNIGMNNGIILTTGTLNPDYVPTIGSGAGWFSSFVN
                     GTAGNDLLNNLVSVTTYDASVLEFDLIPQGNYLEFQYVFGSEEYPEFAYSDFNDVFGF
                     FIDGIDPFGGMYSATNIALLPESSMYVSINNVNCCANSEYFVDNLGIEGQTIVFDGFT
                     TVLIAVVRVEPGTNYHLTLAIADAGDASYDSAVFLMSNSLKSYFLSDIESVNSYAPAI
                     IYNHNSREIEIKAGNNNLEPIRLSIYNLQGSTVYKTSFTGANTVDCSNFTNGIYVIQA
                     CTEKGCKTTKISVF"
     gene            complement(1318..1785)
                     /locus_tag="PLK75_05235"
     CDS             complement(1318..1785)
                     /locus_tag="PLK75_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY38685.1"
                     /translation="MNIRIRTASREDVTAIYAMILELADFEQAPDKVTNTPEKMIQEY
                     DNYRCFVAETEEDGIIGMALFYIAYYTWVGKSLYLDDLYVKPAFRGKKIGTKLLNEVI
                     KIAKNENCSRVRWQVLDWNTAALNMYRNLGAHTDSEWINCDLTVNEINNFPVK"
     gene            complement(1802..2611)
                     /gene="kdsA"
                     /locus_tag="PLK75_05240"
     CDS             complement(1802..2611)
                     /gene="kdsA"
                     /locus_tag="PLK75_05240"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOY38686.1"
                     /translation="MTGKVHEIKQSHRDNFFLIAGPCVVESKEVCFTVAESLLKICTK
                     LDIPLIYKASYRKANRSMASSFTGIGDQEALGILQQVKQEFGLAVCTDIHSIDEAEFA
                     AEIADVLQIPAFLCRQTDLLVAAGNTGRTVNIKKGQFLSPEAMKFAAEKVMQTGNRNI
                     MLTERGSSFGYTDLIVDFRGIPVMKSFGFPVVLDATHSLQQPNQISGVTGGRPELIAT
                     IARAGIAAGVDGLFIETHPDPAHALSDGANMLHLDRVEKLLESLLRIYQAL"
     gene            complement(2680..3999)
                     /locus_tag="PLK75_05245"
     CDS             complement(2680..3999)
                     /locus_tag="PLK75_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOY38687.1"
                     /translation="MKTRANTKFKLILLLSWIIVVTVSCQKKKEYTFATTSKGTSYNE
                     VGKLITDLINKSEDRSFVMLEGSELGSLVNCKKLYKNEVDFAIAQNDTRISGFIGSEG
                     SVVDSKIRTVLPLYPEILFIVYPDTIMASDIESLVTGRRIGVGPNNSGTHRFFKAYLD
                     HCGIDSTKYSFVHTPWSENVVSEKLDISVNVGGYNASAVVDMLNTGKCKIFSLGDYRL
                     YGKGSPVEGFCMNYTTARPYIIPMQTYRFGPDEPVLTLAVDAVLLCRADIDAEEVYRI
                     VEQIIVNAKLLSDKNPLLSGINDNFDQSTLNFPLHEGVTRYLERDEPSFMEKHIDVIA
                     FVFSMLVAAFGTMMAFFRKIKLKKKDRIDEYYNKVLSIEKDMDYMKTTDELGKALGIL
                     HTLKQDAFNALIDEKLVANESFNIFLALTDTLITRVENKIKQATVKV"
     gene            4158..4733
                     /locus_tag="PLK75_05250"
     CDS             4158..4733
                     /locus_tag="PLK75_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY38688.1"
                     /translation="MKKLIIAFLFIMMGIGVYAQENKKIAIIPQVGLNFSKFANEPVE
                     QDYHSRTGYQAGLFISTLNTVFIQPGIFFSRQGNDLYHFSELTSDNIRENVDYNVLKV
                     PVFFGLNLWVFRVYTGPTLSVFTNVHGNQFGFENEDFRSAVFGMNLGLGMHIGIFSLD
                     CHYEFGVSDISPTINNKVNVLTINAGLRFRL"
     gene            complement(4728..6395)
                     /gene="recN"
                     /locus_tag="PLK75_05255"
     CDS             complement(4728..6395)
                     /gene="recN"
                     /locus_tag="PLK75_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOY38689.1"
                     /translation="MLKSISIRNYLLIENLELSIPDGFIVLTGETGTGKSIIIDALGL
                     ILGNRLSTGIQISGDNKCVVEASFNISGKPAIYSMLHENDIDRDDVMLVRREINKTGK
                     SRAFINDTPVNLQTLEAITSRLVDMHNQFANLEIAKPAFRLSIVDSVAGTRKDFADYK
                     LIYSEFTALKNILNEKKQQHEAIIKEADFYEYQLKLLQESGVKSNAEYEELESELNTL
                     MHAGEIKENLYECMLTLSKGDNDMVTQLKNCITIMTKVMRNSDMATPLKERLESLLVE
                     LKDIDSEISALETRVEVNPQRLEMVTGQIDKINSLLSRFRVNTVDELRMLENEFATKI
                     SQASTLTDEVLLLQKECDTLQTKVENAAAVLSGKRSKVFGRIKADLEEMLQSLNLNGA
                     RFEVVNKISQQPDASGIDNIDFLFSANKNMAPENLMQTASGGELSRIMLCLKSLMSSE
                     DAIPTLIFDEIDAGVSGEIADKMGRIMQKLSVGRQVISITHLPQIAARGKSHFKVIKT
                     EKTDRTVTSVKLLNQNERTEELASMLSGAEVTAAARANATNLLQNSL"
     gene            complement(6405..7310)
                     /locus_tag="PLK75_05260"
     CDS             complement(6405..7310)
                     /locus_tag="PLK75_05260"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOY38690.1"
                     /translation="MKTLMIFVFSLFCLLRLSAQELDCRIQVNYSKLQGTSNQQIFTT
                     FQQALYEFLNNTKWTNNVFQKDERIECNFNFNITEQISSDEYKGTLQITYSRPVFGTS
                     YNSPILNHLDNDIQFKYVEFQPLDFNVNSFSSNLTSILAFYVYIIIGLDYDTYGMNAG
                     SEYYSKAEAIVQNAQSAPEKGWKAYENLKNRYWFVENLQNDQYSQVRDFLYVYHRQGL
                     DKMADKPADARARIEESLEYLRNVHRRKQGTFLMQIVTTAKGDEIVNIFSEAFPDEKA
                     RVYNIMKEIDVANVTKYDDIMKNPE"
     gene            complement(7323..8528)
                     /gene="coaBC"
                     /locus_tag="PLK75_05265"
     CDS             complement(7323..8528)
                     /gene="coaBC"
                     /locus_tag="PLK75_05265"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOY38691.1"
                     /translation="MLHGKKILLGLTGSISAYKAAFLARLLVKQGAEVKVIMTPLAKE
                     FISPLTMATLVKNPVLVDFYNPENGDWNSHVDLGLWADAYLIAPATANTIAKMASGIA
                     DNLLLTTYLSVRCPVFVAPAMDCDMFEHPATQQNLKKLASCGVISIEPAEGELASGLT
                     GKGRLEQPENIVNDIIQYFDNQNILKGKKILVTAGPTYEKIDPVRFIGNYSSGKMGYA
                     IAEELAKYGADVILVSGPTHLKVISEHIELIRVNSANEMFQACISFFSGCDAAIMSAA
                     VADFTPLAPARNKINRENSNITIELKPTKDIAAELGKLKKENQILVGFALETEFEKNN
                     ADKKLKKKNFDFIVLNSLQDAGAGFNFDTNKVSIIEKNGSVTDFELKLKTEVASDIAE
                     KVITYLNTI"
     gene            complement(8528..8869)
                     /locus_tag="PLK75_05270"
     CDS             complement(8528..8869)
                     /locus_tag="PLK75_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOY38692.1"
                     /translation="MDFKKTKAPITTVTNNLSDMESKVGNIYEAVVICAKRSNQISQE
                     IKQELSAKLNEFASYSDNLEEVFENREQIEVSKYYERLPKPTLIALDEFLDDKLYYRR
                     PEADSANIESV"
     gene            complement(8877..9668)
                     /gene="bamD"
                     /locus_tag="PLK75_05275"
     CDS             complement(8877..9668)
                     /gene="bamD"
                     /locus_tag="PLK75_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOY38693.1"
                     /translation="MKKRLLYLVLAVVVVITVSCSEYQKLLKSDNYELKYTKAVDYFG
                     KGDYYRCMTLLDELSSVYRGRAEAEDIHYMMAQCYFNNEEYNMAAYYFKTFSEVFPVS
                     RKVEECDYMAAYCFYLLSPGPTLDQTFTLRGIQELQLFLNRYPQSERRDSVNILVDNL
                     YLRLETKAYNNAKMYFDIGDFKAAITSLKNVLIDYPDTQFREQAMYYIVKSAYYLAEN
                     SVEEKKKQRYKDTVEYYQSFVDRYPESRYVKEVEKIYTQSLKLIN"
     gene            complement(9812..15958)
                     /locus_tag="PLK75_05280"
     CDS             complement(9812..15958)
                     /locus_tag="PLK75_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOY38694.1"
                     /translation="MNTLMFKIALFAIIISVFSGEFYQKNVITPPDKTYEELWNEVKE
                     FESKGLPKSALEVVEAIYKKAMAEQNSEQVTKSCLYRYKYMYALEEDGLSKVIKQLEA
                     DINIMPGKSKYFGYMLLAEIYSDYLEMNYYVIGQRTRLSEFTPDDLATWDVSHFKDKI
                     IKNYVLALNNELKTEPIEKYPELIEFTEKGKKSCPTVFDLFSHMAYRLFSTPVSNWYS
                     NNYDRNEAIITNIQYCETAEVFSETDFLANDSISYAFYTHKVLKDWINFRLSDPKSAE
                     ALVFADLIRLNYYRIHVQHPEKDKVWMNTMQALHDKFFKNPVIAEIDILIANYYYAIG
                     SKYSYSDTLSWKNAPYMRKAYDLVDQVVKMYPDTTISAKCMNLKSSIEKKEAGMYSLH
                     NSGSGKNTQVQIYYRNVNKVNLSLLKIDYEDYRKIERKYYDREDKIAELLSKSTIVST
                     EKIDLGNETDMNMHARNYFVKPLPKGHYLYVLHQSDNIADERMLLSYNAIFITDFVYT
                     HRASESGDLEFFVMDRTSGDPVKGAQITFYTEKYNYITSSYNYDRVKQLFTDTNGYVC
                     FNPDDDDYRSYYADIRKGDDFVAVNNYLYSYNYNYNDDDSETVTLFTDRAIYRPGQTI
                     YFKGIATSTKLMVTKLLPKLSTTVELRDPNYQVVGSAQVKTNNFGSFAGSFEIPLGTV
                     TGQFQISCIGGTKYISVEEYKRPNFEVVFKPVEDEYRIGDSIKVTSKVQSYAGSNITD
                     AKVKFTVTRQVSWRGWPYYYSYIPAKEVCFGDVVTDENGDFTVSFRAEPDNEVPGQQN
                     VCFQYSVSATVTDITGETQQGSTYVFATNKSLLLESDLPEMVNKTSIDTLRIYARNIN
                     GKDINTQVKVRFVALEDTKNLLQPDLFGKTDRSGYSKEEWYSLNPGVAYSNENDYRTF
                     RELKTVLSYDVKTGENFKILIANDIRKLNPGRYCLKLQAKDKFGGDVEELKYFTVYDP
                     GVTAMPYMAATLFIQENATVLPGEKAILYLGTSFKDVKLSYELEHKGVIAERKWINVS
                     NEIKKIEIPVTEDFRGNFTVHVLFVKNGRQYTSTFIFYVPWTNKELNIKLETFRDKIE
                     PGSKEEWKIRITDNDGKPVSAEMLAGMYDASLDAFTANSWWLNTNPYYYSFRYWGTSC
                     YSLNGSRFLYTFPMSYAKVPNDYVMFNWNGYSHGGYYYSYDMSGGYEYEGDMRNYDRM
                     AVSDSQSGMAGGREKAAKEEAPSAAYGMAEMSLVDADETVTTATRNGPTSGEGKMGQQ
                     PKIRANFNETAFFYPQMVSDSNGVIMLTFTAPESLTRWNLMALAHTTDLKIGSLNKQI
                     VTQKELMLMPNAPRFIRENDTLIFSAKISNISEKQITGNTTVRLFDAITMREITNELL
                     LSSPDMQKFDVKAGGNVEVNWKISVPSGLAGVVYRITAESETHSDGEEKYLPVLPNRM
                     LVTESIPLPIRGGQTKKFSFNHMKQSIQSSTLTNYKLTLEFTSNPAWYAVQALPYLAE
                     YPYECAEQTFSRYYANSLAAYIANSDPKIKRVFDVWKSTPGSDALKSNLEKNEELKAL
                     LLEETPWVLEAQDETERKHRIALLFDVNQMKNQLSTALSKLKKMQVYNGAWPWFDGMP
                     EDRYITQHIVGGIGHLQKLGVVDVSSNNELYSMAGKAIDYMDNELVKDYKALLKYYTP
                     KEMELNHLGYYQIHYLYVRSFYKDIWPIPEKSKTAFEYYKKQAEKYWLSNNRYMQGML
                     ALALFRFDEKEVASDIVASLKEYSLQSEEMGMYWKSDAGYYWYEAPIETQSLMIEVFN
                     EVSSDSIAVEEMKIWLLKQKQTQDWKTTKATADAIYALLLTGMELLSDDQLVEITLGT
                     EKINPAEMKDLNVEAGTGYFSKFWSGSEIKPEMADITVKKPGKGIGWGAVYWQYFEQL
                     DKITSFKDTPLKIDKKLFVELRKDGKVVMTPVTEKTQLHVGDRVIVRIEIRTDRNMEY
                     VHLKDMRAACFEPESVFSGYRYEHGLGFYQSIRDASMNFFISYLHQGTYVFEYPLIVS
                     QRGEFSNGITTMQCMYAPEFTTHSQGIRVKVQ"
     gene            16128..17003
                     /locus_tag="PLK75_05285"
     CDS             16128..17003
                     /locus_tag="PLK75_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOY38695.1"
                     /translation="MIVSMTGYGRTKVAVNGKNISIEIKSINSKQLDVSLRFPSFYKE
                     KESEIRELLSTKLLRGKIDFALYAELDPGFQPTIINHELASKYIAEMISLCEKHNISP
                     GPEIISKALQMPDVMTSRNDELSTDEWEIIKCACKEAVEQLTEFRKSEGNVIGCDIML
                     HINNILDLLIQVEKFEKQRISFIKEKLLQQLSELMRQSDIDGGRLEQELIFYNEKLDI
                     TEEKIRLKKHCDYFKETFNTEENSGRKLGFISQEIGREINTLGSKANNSDIQIIVVQM
                     KDELEKVKEQLLNVL"
     gene            17016..18263
                     /locus_tag="PLK75_05290"
     CDS             17016..18263
                     /locus_tag="PLK75_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOY38696.1"
                     /translation="MTAEFIFYLILTILIAGFLIDMFLSYLNYRSSRQPIPQLLDGIY
                     DNNKYLEQQAYMKANHRFGNVNSLFSLIVILLMLIFDGFSYADNLARTIGVSEVFTSL
                     IFFALLAFANEILSIPFDVYDTFVIEKKFGFNKTSPKIYITDKIKSWLLMAIIGGGLI
                     TLITWVYYYTAEYFWVLTWVAIFLFSIVANFLYSDLIVPLFNKQKPLEEGELRTAISD
                     FAAKAGFSLKKIMVIDGSKRSTKANAYFSGFGSRKRVVLYDTLIAEMQTDEIVAVLAH
                     EIGHYKKKHVLINLIVSSVSTACVLYLLSLFLKFPEFSQAINIENASFHSGLLVFGIL
                     FTPISALAGIFTNMSSRRHEFSADKYATMQYHANSLISALKKLSSHNMVNLTPHPWFV
                     FVNYSHPPLLHRIKRINEIEAKS"
     gene            18428..19360
                     /locus_tag="PLK75_05295"
     CDS             18428..19360
                     /locus_tag="PLK75_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOY38697.1"
                     /translation="MISYWQIITNGITPISEFTKECIVNVVNPSTAEFNILTTTFGLP
                     EDLIADVLDLDERSRIEFEDDFVFMIMRIPVINNESGIPFNTIPMGVFIKGQNIFVLC
                     QKENQVINGFFNKQMRRKFFPQNKVDLVLYLFLHANNLYHHYLREINAQTNLVEKDLE
                     RSIRNKELQGLLKLEKCLVYFTTSLRSNEYILAKLKNSSFIRNNDFSEDLLEDVIIEN
                     KQAIEMSNIYSDIQSGMMDAFASVISNNLNIVMKRLTAISILLMIPTLIASLFGMNVP
                     NFMEDSNLALVGILLFSVLLSITGLLIFRKKDWF"
     gene            19488..19853
                     /locus_tag="PLK75_05300"
     CDS             19488..19853
                     /locus_tag="PLK75_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY38698.1"
                     /translation="MKNFDSFPLLKMSLNYALGVIDFSGELEDARKKVIAKKLLEAGF
                     AISSNLYQARKAERQTDYLFSMKNALRNTDESIYWLKQCLNSENYPKDPELTMMGQML
                     MKKIEENLRTQTPDFRKDT"
     gene            19996..23274
                     /locus_tag="PLK75_05305"
     CDS             19996..23274
                     /locus_tag="PLK75_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOY38699.1"
                     /translation="MKYAALVIFVLCFLSVQSKEFSRLLRFPDVYDNQVVFTYAGDLY
                     TGELAGGTARRLTSGQGNEIFAHFSPDGSMIAFTAQYDGNTEVYVMPSEGGTPLRLTY
                     TATLSRDDLSDRMGPNNIVMGWSHDGKKVIYRSRKATFNDFVGHLFMVPVEGGMSEQI
                     PFSTGSWCSFSDDGMMIAFNRVFREFRTWKYYRGGMADDIRIHDFRTQTTENITNNDA
                     QDIFPMWHKNLVYFLSDRDRTMNLFAYDTITKKTSKLTNFTLYDIKFPNIGNNGIIFE
                     NGGWLYYYSFEKKNVELLYITVSEDNSGSRISYKNASERINSWSLSPDGVRIAFGARG
                     DVWSVPAEEGISRNISSTSGVHERNVRWSPDGKYIAYISDETGETEVFYSKADGSEKP
                     VQVTKAADTYTYSLKWSPDSKKILYHDKRMRLQYTDIVSGSTVLVCASEVTETGDYNW
                     SPDSKWIAYMNSDTSRIAKIFLFSTETKKSHQITSDWYDSYNPVFSDCGKYLFFVSDR
                     DFNPIYSETEWNHAYQDRAKIYFITLNRNDLSPLAPKNQEVEVAPDEVKNIKTDKGKS
                     REKEKENENSNAVKPVVIDLENIGQRISALPVQAGNYWNLCVFDNKVYYNYSRMNESA
                     SLRLFDLSKKEEKNLGNYSSFEISINRKKMALPQSGNYYIIDLPTANINVNQSASVNL
                     DNMYVWVDLQQEWNQIFEETWRQMRDFFYDPGMHGVSWADIRKKYEPLVAFVNNRHDL
                     NYILGEMIGELNIGHAYTGGGECPQPQRVKTGLLGAEISKHESGYFRIDKILLGESWV
                     SNCRSPLVGQGMEITEGDFIIAINGKYLKDVPDIYSLLVGKADVQVEITFSSKPDGKN
                     TRKYIIVPVDSEDKLYYYTWVQHNIDYVNKATNGQVGYIHVPDMGQEGLNEFVKYFYP
                     QLAKKAIIIDDRGNGGGNVSPMLIERLRREVSMMAMGRNADIPYTKPRELLNGPKVLL
                     INQYSASDGDLFPYQFKKHKLGKVIGVRTWGGVVGIRGSLPFIDGGLLMKPEFAHYSA
                     EGKGWIIEGYGVDPDIYIDNDPYLEFTGVDTQLDKAIEVALEELKNNNYNLPGIPEFP
                     DKSR"
     gene            23381..23902
                     /locus_tag="PLK75_05310"
     CDS             23381..23902
                     /locus_tag="PLK75_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38700.1"
                     /translation="MKNFMYLFLALSISIAVVSCGNKKDKEETKKAENLADLKTKYDG
                     KQFKDCDEYFAAADEMLDVMVATVKKADAGDEKAKAEFKDLVSFLGQFETEGEKYKTE
                     CPDKFKEFEKKSDEKAKPIEEAIMKLTFDMMQDEMPSVEDSLQAISNEVENKIEGIQT
                     EGNNKLDALKKKK"
BASE COUNT         7066 a         4933 c         4617 g         7476 t
ORIGIN      
        1 gaagaattga tgcaaaaaca atacgtgctt ggtgtttcaa aatattttag tggtcatccc
       61 gtaaaacttc aggggaatat catgtatatc gataacgatt cgtataaccc cagcattccc
      121 ggaaagagta atttcggttt tatgcttcaa atggaattgg gaatataatt ctataaaaga
      181 cctttccttc acaagtttgt attatatatt ccgttatatt tgtatttggt attaaatcac
      241 caattttaaa aacaagctta tgaaaactat actcgctttt tcgacagtaa tagctttgac
      301 attgctttgc agatcagtgt atgcacaatt agttacgact acaaacgtag acccggcaca
      361 aacagtttca gagtttatat taaatggtgt tgaagcatca aatgttgttt acacaggtga
      421 tgtcaatgca tttggtactt tcaccggggg atacgatacc aatattggga tgaataatgg
      481 gattatctta acaacgggta cgcttaatcc ggattatgta ccaacaatag gtagcggagc
      541 gggttggttt tcttcttttg ttaacggtac ggctgggaat gatttactga acaacttggt
      601 ttcggtaacc acctacgatg cttccgtact ggaatttgac ctgattccgc aaggtaatta
      661 tcttgaattt caatatgtat ttggatcaga agaatacccc gaatttgcgt actccgattt
      721 caacgatgta tttggatttt ttatcgatgg catcgatccc tttggtggga tgtattccgc
      781 aaccaatatt gcattattgc ctgaatcatc aatgtatgtt tctataaata atgtaaactg
      841 ttgtgcaaac tccgaatact ttgtcgacaa tttgggtata gaaggacaaa caattgtttt
      901 tgacgggttt acaactgttc tcattgcagt agttcgagtt gaacccggca ccaactatca
      961 tcttacattg gccattgccg atgccggtga cgcttcatac gactcagctg tatttttaat
     1021 gtcgaacagc ctgaaaagct atttcttatc agatatcgaa tcggttaatt catacgctcc
     1081 tgccattata tacaaccaca attcacgcga aattgaaatt aaagctggca ataacaatct
     1141 ggaacccatt agactatcga tctataatct gcagggcagc acagtttaca aaaccagctt
     1201 taccggtgca aataccgtgg attgcagcaa ctttaccaat ggaatatatg tgatacaggc
     1261 ttgcactgaa aaaggctgca aaacaacaaa aatttcagtt ttctagtcct ttcttaccta
     1321 tttaacaggg aaattgttga tttcatttac agttaaatcg cagtttatcc attcactgtc
     1381 ggtgtgagcg cctaaattac ggtacatgtt cagcgcagcg gtattccagt cgagtacctg
     1441 ccaacgcacc cggctacaat tttcattttt tgcaattttt ataacttcat tcaggagctt
     1501 cgttccaatt ttctttcccc taaaagctgg tttaacatat aaatcatcga gatagagcga
     1561 ttttccaacc catgtgtaat aagcgatata aaacaacgcc attccgataa taccgtcttc
     1621 ttctgtttcg gcaacaaaac agcggtaatt gtcatattcc tgaatcattt tttcaggggt
     1681 gttggttacc ttgtcggggg cttgctcaaa atcggcaagc tcaaggatca ttgcatatat
     1741 tgcagtgaca tcttcgcgac ttgctgtacg gattctgata ttcataagta agaatctctg
     1801 attataaagc ctgatatata cgcaacagac tttccaatag tttttcaacc ctgtcgaggt
     1861 gaagcatgtt ggcgccatcg ctgagcgcat gggcagggtc ggggtgcgtt tcaataaaaa
     1921 gtccatccac tcctgctgca atacctgcac gagcaatagt tgcaatcaat tcaggccgtc
     1981 cacctgtaac acccgatatc tgattgggtt gctgcagact atgcgtagca tcaagcacca
     2041 ccggaaaacc aaaagatttc ataaccggaa tcccacggaa gtcaacaatc aggtcggtat
     2101 atccaaaact tgaccccctt tctgtaagca ttatattcct atttcctgtt tgcattactt
     2161 tttctgctgc aaatttcata gcctctggcg aaaggaattg tcctttttta atgttaaccg
     2221 ttctacccgt atttcctgca gcaacaagca aatcggtttg ccggcataaa aacgcaggga
     2281 tttgtaaaac atcagcaatt tcagcagcaa actccgcttc atcaatactg tgtatatcgg
     2341 tgcaaacagc caatccaaat tcctgcttca cctgctgcag aattccgaga gcttcctgat
     2401 cgccgattcc agtgaacgag gaggccatac tacggtttgc tttgcggtaa gaggccttat
     2461 atatcaaagg aatatcaagt tttgtgcata tttttagcaa actttccgca acggtaaaac
     2521 agacttcctt tgactcaacc acgcaagggc ccgctatcag aaaaaagtta tcgcgatgcg
     2581 attgttttat ttcatgtact ttaccggtca taagccaatt tttccgaaat ataataaaaa
     2641 gctgttataa ttgaaataat tgctttcgta agccatgtat cataccttta ccgtagcctg
     2701 ttttatttta ttctcaacac gtgtaatcag tgtatcggta agtgccagaa aaatattaaa
     2761 gctttcgtta gcaaccagtt tttcatcaat aagcgcatta aaagcatcct gcttgagagt
     2821 gtgcaaaatt ccaagtgcct tacccagttc atcggtagtt ttcatatagt ccatatcctt
     2881 ctcaattgag agaaccttat tgtagtactc atcaatccta tctttctttt tcagttttat
     2941 ttttctaaaa aaagccatca ttgtaccgaa agcggccacg agcatcgaaa aaacaaaggc
     3001 aataacatcg atatgtttct ccataaacga aggttcatca cgttcgagat acctagtaac
     3061 accctcatgc agagggaaat tcagcgtact ttgatcaaaa ttatcattaa tacccgaaag
     3121 caaaggattt ttatcggata aaagcttggc atttacaatt atttgttcaa caatccggta
     3181 cacttcttca gcatcaatgt cagccctgca aagcagtaca gcatccacag ccaaagttag
     3241 caccggttca tccggaccaa aacggtaggt ttgcatgggg ataatataag gccgcgctgt
     3301 agtataattc atacagaagc cttctaccgg agacccttta ccatagagac ggtaatcgcc
     3361 aaggctgaat atcttgcatt tgcccgtgtt gagcatatca accacagcag aagcgttata
     3421 gcctcccaca ttcacactga tatcaagctt ttcggaaact acattttccg accagggagt
     3481 gtgtacaaat gaatattttg tgctatcgat accgcaatgg tcgagatatg ccttaaaaaa
     3541 cctgtgtgtt ccgctgttgt taggtcccac tccaattcgc cttccggtaa ccagactttc
     3601 tatatcagaa gccataatgg tgtcgggata aactatgaat aaaatttcgg gatataacgg
     3661 aagtacagta cgaattttac tgtcaacaac cgaaccttcg ctaccaataa atcccgaaat
     3721 cctcgtatcg ttttgggcta tagcaaaatc aacctcgttt ttatacaact tcttacagtt
     3781 gactaatgaa cccagctcac ttccttcgag cattacaaag ctgcgatctt cacttttgtt
     3841 tattagatcg gtaatgagtt ttccgacttc gttgtatgac gttccttttg aagttgttgc
     3901 aaaggtgtac tcttttttct tctgacaaga aacggtaaca acgataatcc aactcaaaag
     3961 aagtatcagt ttgaatttag tatttgccct tgttttcatg gtaaaaggca ttaatctttt
     4021 gtgattaata atgcaattat ataaaaatta agaagaacta attcggtaat aaacctggtt
     4081 tgttgaaaat attaatttga aaagaaatta tttctcttat ctggcaatat ctttgtattt
     4141 gaaaacaaaa taaagtcatg aaaaagctaa ttattgcgtt cttgtttata atgatgggta
     4201 taggtgtgta tgctcaggaa aataagaaaa ttgccatcat tccacaggtg ggtctaaatt
     4261 tctctaagtt tgccaatgaa ccggttgagc aggattatca ttcgcgcacc ggatatcagg
     4321 cagggctttt tattagcacc ctgaacactg tttttattca acccggcatt tttttctcaa
     4381 ggcagggcaa cgatttatat cacttcagtg aattaacctc cgacaacatt cgtgaaaatg
     4441 tggattacaa tgtcctgaaa gttcctgttt tctttgggtt aaacttgtgg gtttttcgtg
     4501 tttacactgg tccaacccta tcggttttca caaatgtgca cgggaatcag ttcggttttg
     4561 aaaacgaaga tttcaggagc gctgtgtttg gaatgaatct tggacttgga atgcacatcg
     4621 gaattttcag tctcgactgt cattatgagt ttggtgtatc tgatatatct cccaccataa
     4681 acaataaagt gaatgtttta acaattaatg ccgggcttcg attccggtta taaggaattc
     4741 tgcaacaggt tcgtggcatt tgcacgggca gcagcggtta cttcagctcc gctaagcatc
     4801 gacgccagct cctcggttct ttcattctga ttgagcaatt tcacagaggt taccgttctg
     4861 tcagtctttt cggttttaat aactttaaaa tggctctttc ctctggcagc tatctggggc
     4921 aaatgtgtaa tactgattac ctgcctgccg accgaaagtt tctgcataat tcttcccatt
     4981 ttatcggcta tttcgccgga tacccctgca tcaatttcat cgaaaataag tgttgggatt
     5041 gcatcttccg acgacatcaa cgatttcagg caaagcataa tgcgcgataa ttcgccgcct
     5101 gatgcggttt gcatcagatt ttcaggtgcc atattcttgt tggcactgaa taagaaatcg
     5161 atattatcaa tcccagacgc atcgggttgc tgcgagattt tgttaacaac ttcgaagcga
     5221 gctccattca gatttagtga ttgaagcatc tcttccaagt cggcctttat cctgccaaaa
     5281 acctttgagc gtttgccact taataccgca gcagcgtttt caactttggt ctgaagcgta
     5341 tcgcactctt tttgcagcaa aagtacttca tcagtaagcg ttgaggcctg actgattttg
     5401 gttgcaaact cattctccag catccggagt tcatccacag tatttacccg aaaacgcgac
     5461 aacaatgaat tgattttatc aatctggcca gtaaccattt caagtcgctg cggattgact
     5521 tcaacacgtg tttccagagc actgatttca gagtcgatgt ccttcaactc aacaaggagc
     5581 gattcgaggc gctctttcaa cggagttgcc atgtcggaat tgcgcataac ttttgtcatg
     5641 attgttatgc agtttttcaa ctgagtaacc atatcgttat cacctttcga taaggtaagc
     5701 atgcattcat acaaattttc tttaatctct cctgcatgca ttagggtgtt caattccgat
     5761 tcaagctcct cgtattcggc gttgcttttt actccgcttt cctgtaaaag cttaagttgg
     5821 tattcgtaaa aatcggcctc tttaataatt gcctcatgtt gctgcttctt ttcgttcagt
     5881 atatttttaa gcgcagtgaa ttcactataa atcagtttat aatccgcaaa atctttcctt
     5941 gttccggcta cactgtcaac aatactcagg cggaacgcag gtttcgcaat ttcgagattg
     6001 gcaaactggt tatgcatatc aaccaacctt gaggtaattg cttcgagggt ttgcagatta
     6061 accggtgtgt catttataaa agccctcgat ttccctgttt tgtttatttc gcggcgtacc
     6121 agcataacat catccctgtc aatatcattt tcgtgcaaca tcgaataaat agcgggtttc
     6181 cccgatatat taaatgatgc ttctacgaca catttattat ctcccgaaat ttgtatgcct
     6241 gtgctaagcc ggttgccaag tatcaggccc agcgcatcaa ttataattga tttaccagtc
     6301 ccagtttctc ccgtaagaac aataaagcca tccggaatac tgagttcaag attttcaatt
     6361 aacagatagt ttctgattga aattgatttc aacatacgaa attatcattc cgggtttttc
     6421 atgatatcgt cgtatttcgt aacattggca acgtcgattt ctttcatgat attatacact
     6481 ctggcctttt cgtcgggaaa agcttcgctg aaaatattta cgatttcgtc gccctttgca
     6541 gtcgtaacaa tctgcattaa aaatgtgccc tgtttcctac ggtgaacatt cctcagatat
     6601 tcgagtgatt cctctattct tgcgcgtgca tctgcaggct tatcggccat cttatcgaga
     6661 ccctggcggt gatatacgta caaaaagtcg cgcacctgtg aatactgatc attctgcaga
     6721 ttttcaacga accaatatct gtttttcagg ttttcgtatg ccttccatcc tttttctggt
     6781 gcagactgag cattttgaac aatggcttcc gcttttgaat agtattcgct tcctgcattc
     6841 ataccgtagg tatcatagtc taaaccaatt atgatataca cataaaatgc aagaattgat
     6901 gtaaggttcg atgaaaaact gtttacgtta aaatcgagcg gctgaaattc aacatactta
     6961 aactgaatat cattgtccaa atgattcagt atcgggctat tataagaggt tccaaataca
     7021 ggtcggctat aggtaatctg cagtgttccc ttgtactcat cagaagatat ttgttcggtt
     7081 atgttaaaat tgaaattaca ttcaatccgt tcgtctttct gaaaaacatt gttggtccat
     7141 tttgtattat tcaggaactc atataaagcc tgttgaaagg ttgtaaatat ttgctggtta
     7201 ctggtcccct gcaatttgga gtaatttacc tgtatgcggc agtcaagctc ctgtgcactg
     7261 agtcgcaaga ggcaaaacaa cgaaaaaacg aaaatcatca aggttttcat aaactgatgc
     7321 ttttaaatgg tattcagata tgtaattact ttttcggcaa tatcgcttgc cacttcggtt
     7381 ttgagtttca attcgaaatc ggtaaccgac ccattctttt cgataatact aaccttgttg
     7441 gtatcgaaat taaaacctgc acctgcgtct tgtaacgaat ttagcacaat gaagtcgaag
     7501 tttttctttt tcagcttttt atcggcattg tttttctcaa attctgtttc cagagcaaaa
     7561 cccacaagaa tctgattttc ttttttgagt ttacccaact cggcagcgat atcttttgta
     7621 ggttttaatt caatagtgat attgctgttt tcgcgattaa ttttgtttct ggcaggagca
     7681 agcggggtaa aatcagcaac agcagcactc ataattgcag catcacagcc tgaaaagaaa
     7741 ctaatgcatg cctgaaacat ttcattcgca ctattcactc gaatcaattc aatatgctca
     7801 gaaataactt tgaggtgtgt cggaccgcta accaaaatta catctgcacc atattttgca
     7861 agttcttctg ctatggcata tcccatttta ccggaggagt agtttccgat aaaacgaacc
     7921 gggtcaatct tttcataggt tggtcctgct gttaccaaaa ttttttttcc tttcagaatg
     7981 ttttgattat cgaaatactg gataatatca ttgacaatat tttcaggttg ctccaatctt
     8041 ccttttcccg tcaatccgct tgccaattct ccttcggctg gttcaattga aataacacca
     8101 caagaagcaa gttttttcag gttttgttga gtagccggat gttcaaacat atcgcagtcc
     8161 atagccggag ctacaaaaac agggcatcta acagagagat acgtagtaag cagtaggtta
     8221 tcggctatgc cggaagccat tttagcgata gtatttgccg tagcaggtgc gattaaatag
     8281 gcatcagccc ataagcccaa atccacatga ctattccagt ctccattttc agggttatag
     8341 aaatcgacaa gtactggatt tttaacaaga gttgccatag taagcggtga aataaactct
     8401 ttagccagag gcgtcataat cacttttacc tcagctccct gctttactaa taatctggcc
     8461 agaaaagcgg ctttgtaggc agaaatactc ccagttaaac ccagtaagat tttcttgcca
     8521 tgcaacatca caccgattca atattagcac tatctgcttc aggacgccga taatacaatt
     8581 tatcatcaag aaattcatca agtgctataa gcgttggctt cggcagtctt tcatagtatt
     8641 tgcttacttc aatctgctcc ctgttttcga aaacttcttc aaggttatcg ctgtatgatg
     8701 caaactcgtt cagcttggca ctcaattcct gtttaatttc ctgcgatatc tggttcgacc
     8761 tttttgcgca aatcacaaca gcttcgtaga tattgccaac tttgctctcc atatcgctca
     8821 ggttgttggt cacagtggta atcggtgctt ttgttttttt aaaatccata attatgtcag
     8881 tttatcagct ttagtgattg tgtatatatt ttttcgactt ctttcacgta tctgctctcc
     8941 ggatacctgt caacaaatga ctgatagtat tctacggtat ctttgtagcg ctgtttcttc
     9001 ttctcttcca cactattctc cgcaaggtaa taggctgatt ttacgatata gtacatggct
     9061 tgctcacgga attgtgtatc gggataatcg attagaacat tcttcagaga tgtaatggcg
     9121 gctttaaaat caccgatatc aaaatacatc ttggcattgt tgtaggcttt ggtttcgagc
     9181 ctgagatata aattgtccac gagtatattc acgctatcac ggcgttctga ctgaggatac
     9241 cggttcagaa aaagctgaag ttcctgaatg ccccttagtg taaaagtctg gtcaagtgta
     9301 ggcccgggcg ataaaaggta aaaacagtag gcagccatgt aatcgcattc ctcaactttc
     9361 cggcttactg gaaaaacttc agaaaaggtt ttaaaatagt acgctgccat attgtattct
     9421 tcgttattaa aataacattg ggccatcata tagtgaatat cttcagcctc tgccctgccc
     9481 ctgtatacgc tgctcaattc gtccaacaat gtcatacacc tataatagtc acctttcccg
     9541 aaataatcaa ctgctttagt gtatttcaac tcataattgt cgctttttag cagtttctga
     9601 tattcggaac aagaaacagt aataacaact acaactgcca gtacaagata taaaagcctt
     9661 tttttcatcg ctgcaaatat aaacttaaat acttaactat aaaagctttt ttttattgcc
     9721 gctgcaggat aaaaaacatc gtaacctgag agcatacccg gaagcaaaaa aaaagagcat
     9781 tactgctcct tttttttgat atccttgcag tctattgcac tttgacacga atgccctgac
     9841 tgtgtgtggt aaattcgggg gcgtacatac attgcatcgt agtaatgcca ttgctgaatt
     9901 ctcctctctg agaaactatc aggggatatt caaagacata ggtaccctga tgaagatatg
     9961 aaataaagaa attcattgac gcatcccgga tgctctggta gaaacccaat ccgtgttcat
    10021 atctataccc cgagaacacc gattccggtt cgaaacaggc agcacgcata tctttcagat
    10081 gaacatattc catgtttctg tctgtccgta tctcaattct tacaataacc ctgtcgccaa
    10141 catgaagctg agttttttct gttacagggg tcattactac tttaccatcc ttacgcagtt
    10201 cgacaaacag tttcttatcg atttttaacg gtgtatcctt gaacgatgta attttatcta
    10261 attgctcaaa atattgccaa tacactgcgc cccatccaat ccctttaccg ggctttttta
    10321 ctgtaatatc tgccatttcc ggtttaattt cagagccact ccaaaatttt gaaaaatatc
    10381 ccgttcctgc ttccacgttc aagtctttca tttcggccgg gttaatcttt tctgttccca
    10441 gtgtaatttc aaccaactgg tcatcgctga gcaattccat accggtaagc aacagagcat
    10501 atatagcatc agcggtggct ttggttgtct tccagtcctg tgtctgtttt tgtttcagca
    10561 accatatctt catttcttca actgcaatac tgtcggagct tacttcattg aaaacttcaa
    10621 tcatcagcga ctgtgtttcg ataggtgcct cataccaata gtatccggca tcggatttcc
    10681 agtacatacc catttcttca ctttgcagtg aatactcttt cagtgatgcc acaatatcgg
    10741 aagcaacctc tttttcatcg aacctgaaga gtgcaagggc caacattccc tgcatgtagc
    10801 gattgttgct caaccagtat ttttcggctt gctttttata atattcgaag gctgtttttg
    10861 acttttccgg tattggccat atatctttat aaaagcttct cacatagagg taatgaatct
    10921 gataatatcc cagatgatta agttccattt ctttgggagt ataatacttc agcagggctt
    10981 tgtaatcttt taccaattca ttgtccatat aatcgatagc ctttcctgcc attgaataca
    11041 gttcgttgtt tgatgaaaca tcaaccacgc ccagtttttg caaatgacct atgcctccta
    11101 caatatgctg tgtgatatag cgatcttccg gcattccgtc aaaccatggc catgctccgt
    11161 tgtacacctg cattttctta agttttgata atgcggtact caattggttt ttcatctggt
    11221 taacatcgaa aagcagggca atacgatgct tgcgttctgt ttcatcctga gcttcaagca
    11281 cccagggggt ttcttccagc aacaaggctt tcaactcttc gtttttctcc agattcgatt
    11341 tcagcgcgtc gctgccggga gtgcttttcc acacatcgaa tacccttttt atttttgggt
    11401 cgctgttggc aatatatgcg gctaggctat ttgcataata ccggctgaaa gtttgttcgg
    11461 cacattcata cgggtattct gccagataag gtaacgcttg aacagcatac catgcagggt
    11521 tcgatgtaaa ttcaagggta agtttataat tcgtcagagt tgatgactga atagattgct
    11581 tcatgtgatt aaaactgaac ttttttgtct gtccccctct tattggcaaa ggtattgatt
    11641 cggtaaccaa catacggttt ggcagtacgg gaaggtattt ctcttcgccg tcgctgtggg
    11701 tttcgctttc ggcagtaatt cggtaaacaa ctcctgccaa gcctgatggt accgagattt
    11761 tccagtttac ttcaacattg ccacctgctt taacatcgaa tttctgcata tcgggtgatg
    11821 acaacaacaa ttcgtttgta atttcacgca ttgtaattgc atcgaatagg cgaacagttg
    11881 tattgccagt aatttgtttt tcgctgatat tacttatttt tgccgaaaat atcaacgtat
    11941 cgttttctct gataaatctg ggggcattag gcataagcat aagctctttc tgcgtaacaa
    12001 tctgtttatt taaagagcca atttttagat cggtggtatg tgcaagcgcc atcaggttcc
    12061 agcgggtaag gctttcgggg gcagtaaatg taagcataat aactccattg gagtcgctta
    12121 ccatttgcgg ataaaagaat gctgtctcgt taaaattggc tcttatcttt ggttgctgcc
    12181 ccattttacc ttctcctgaa gtaggtccgt ttcgagttgc tgttgttact gtttcatcag
    12241 catcaacaag gctcatttct gccataccgt aagcagctga gggagcttct tcttttgcag
    12301 ctttttctct tccgcctgcc atgccggatt gactgtccga taccgccata cggtcataat
    12361 tcctcatatc gccctcatat tcatacccac cactcatatc gtaactgtaa taatatcccc
    12421 catgagaata accattccag ttaaacatta cataatcatt ggggactttg gcatagctca
    12481 ttgggaatgt atataaaaag cgggatccat tcaaactata gcacgatgta ccccaatagc
    12541 ggaaagagta ataataagga ttggtgttga gccaccacga attggctgta aaagcatcga
    12601 gagaagcatc atacatgcct gcaagcattt cggctgagac aggttttcca tcattatctg
    12661 taattctaat tttccattcc tctttagaac cgggttcaat tttatcgcga aaggtttcga
    12721 gcttaatgtt cagttctttg tttgtccacg gcacataaaa tatgaaggtc gatgtgtatt
    12781 gtctgccgtt tttaacaaag agcacgtgaa cagtaaaatt cccacggaaa tcttcggtaa
    12841 ccggaatctc tatttttttg atttcgttgc ttacattaat ccattttcgt tctgcaataa
    12901 cgcctttgtg ttcaagctca tacgacaatt taacatcttt gaatgatgtg ccgagataca
    12961 atatcgcttt ttcccctggt aatacggtag cgttttcctg aatgaataag gtcgctgcca
    13021 tatatggcat tgcagttaca cccgggtcgt acacagtaaa atatttcagt tcttcaacat
    13081 cgccaccaaa tttatctttt gcctgcaatt tcagacaata tcttcccggg ttaagtttac
    13141 gtatatcgtt ggctatcagt attttgaaat tttccccggt tttcacatca taactcagaa
    13201 ctgttttcaa ctcacggaag gttctgtaat cgttttcgtt ggaataggca acacccggat
    13261 tcaaactgta ccattcttct tttgagtatc ccgacctgtc agttttgcca aacaaatcag
    13321 gctggagcag gttctttgta tcttccagag ccacaaatct cacctttacc tgagtattga
    13381 tatctttgcc attaatgttt ctggcgtata ttctcagcgt atcaatactg gttttgttaa
    13441 ccatttcagg tagatctgat tcaagcaaca agcttttatt ggttgcaaaa acgtaagtag
    13501 aaccctgttg tgtttcaccg gtaatatcgg taactgttgc gctaacagag tattggaagc
    13561 aaacattttg ctgccccggc acctcgttgt cgggctctgc cctgaagctt accgtaaaat
    13621 ctccattttc atcagtaacc acatccccaa aacaaacctc ttttgcggga atatatgaat
    13681 aatagtatgg ccagcccctc catgaaacct gacgtgtaac agtaaatttc acttttgcat
    13741 cggtgatgtt agaacctgca tacgactgca cttttgaagt aactttgata ctgtcgccaa
    13801 ttctgtattc atcttccacg ggtttaaaaa ccacttcgaa gttgggacgt ttatattctt
    13861 caacactaat gtattttgtt cctccgatac aggatatctg gaactggccg gtaacggttc
    13921 ctaaagggat ttcgaaacta cctgcaaaac ttccgaagtt attggtttta acctgtgcac
    13981 tgcccaccac ctgataattc ggatcgcgca actcaactgt ggttgaaagt ttgggaagta
    14041 attttgtaac cattaatttt gtactggttg caattccttt gaaatatatt gtttgccccg
    14101 gacgataaat agccctgtcg gtaaaaagtg tcaccgtctc ggagtcatcg tcgttataat
    14161 tgtaattgta ggaatataaa taattgttta cagcaacaaa atcgtcaccc tttctgatat
    14221 cagcataata gcttctgtag tcatcatcat ctggattaaa acaaacataa ccgtttgtat
    14281 cagtgaaaag ctgctttacc ctatcgtagt tataagagct ggtaatgtaa ttatatttct
    14341 cagtgtaaaa agttatctga gcacctttta cagggtctcc ggatgttcta tccattacaa
    14401 aaaactccag gtcacctgat tcgctggccc tgtgagtata aacaaaatct gttataaaaa
    14461 tggcattata cgacaaaagc attctttcgt cagcaatatt atcggattga tgtaacacat
    14521 acaaatagtg gcctttgggc aacggtttaa caaaatagtt cctcgcatgc atgttcatgt
    14581 cggtttcgtt gccgaggtca attttctcag tgcttacaat cgttgacttc gacaataatt
    14641 cagcaatttt atcttccctg tcgtagtatt ttctctctat tttgcggtaa tcttcatagt
    14701 cgatctttag caagctcagg ttaactttat tcacattccg gtaatatatc tgaacttgag
    14761 tgttttttcc gcttccacta ttatgcaatg aatacattcc ggcttccttc ttttcgattg
    14821 aagatttcag gttcatgcac tttgccgata tggtagtgtc gggatacatt ttcactacct
    14881 gatcgaccaa atcgtatgct tttcgcatgt agggagcatt tttccaggat aatgtatcgc
    14941 tgtaagagta tttcgagcca atggcgtagt agtagtttgc aatgagtata tcgatttctg
    15001 caattaccgg attcttaaaa aatttatcgt gtaatgcctg catggtattc atccatactt
    15061 tatccttttc gggatgctga acatgaatcc ggtagtaatt cagccggatc aggtcagcga
    15121 aaaccagtgc ttccgcggat tttgggtcac tgagcctgaa attaatccaa tctttcagca
    15181 ctttgtgcgt atagaaagca taagatattg aatcgtttgc aagaaaatcg gtctctgaaa
    15241 aaacttcagc agtttcacag tattgaatgt tggtaataat cgcttcattt ctgtcgtagt
    15301 tgttgctgta ccagtttgaa accggagttg aaaaaagcct gtaagccatg tgcgaaaaca
    15361 aatcgaaaac tgtcggacat gatttctttc ctttttcggt aaattcaatg agttccgggt
    15421 atttttcgat gggctccgtt ttcagttcat tgtttaatgc cagcacatag tttttaatga
    15481 tcttatcttt aaaatgacta acatcccagg tggccaggtc atcaggtgta aattcgctca
    15541 ggcgtgttcg ctggcctatg acataataat tcatttcaag gtaatcgctg taaatttctg
    15601 caagaagcat atacccgaaa tatttcgact taccgggcat gatgttaata tccgcttcaa
    15661 gttgttttat tactttgctc aaaccatctt cctcgagagc atacatgtat ttatacctgt
    15721 acaaacagct ttttgtaacc tgttcgctgt tttgttccgc cattgctttt ttgtaaatcg
    15781 cctctaccac ctctaaggct gattttggca gacctttcga ttcgaattcc tttacttcat
    15841 tccataactc ttcatacgtt ttatcgggag gggtaattac gtttttctga tagaactcac
    15901 ccgaaaaaac cgaaataatt attgcaaaca aggcaatttt aaacatcaaa gtattcatag
    15961 taaagatttt ttagttaaca cgacttagat gttaaagatg caaaaatttc acatgcttcg
    16021 ttatattttt tttcaactat ttttaatttc tcggtaattg caatttgtta agaaatcgct
    16081 tcctaaattg aatcaataca ttacatttgc aaataaaaca aagcgatatg atagtttcga
    16141 tgacgggcta cgggcgcacc aaagtagcag tgaatggcaa aaacatcagt attgaaatca
    16201 aatcaatcaa cagcaagcag ctcgatgttt cgttgcggtt tccttcattt tataaagaaa
    16261 aagaatcaga aatcagggag ttgctatcta caaaacttct tcgcggtaaa atcgactttg
    16321 ccctttatgc cgaactcgac cccggttttc aaccaacaat aatcaatcac gaactggcat
    16381 cgaaatatat agctgaaatg atttcgcttt gcgaaaagca caatatttct cccggcccag
    16441 aaataatttc aaaagcatta caaatgcctg atgtaatgac ttcaagaaat gatgaacttt
    16501 cgaccgacga atgggaaatt attaaatgcg cctgtaagga agcggttgag cagttaactg
    16561 aattccgaaa aagcgaaggc aatgttattg gttgtgatat tatgctccac attaataata
    16621 tactcgattt gctaattcag gtagaaaaat tcgaaaagca gcggataagt tttattaaag
    16681 aaaagttatt gcaacagctt tcagaattaa tgcgacaatc tgatatagat ggcggaaggc
    16741 tcgagcagga acttattttt tataatgaaa aactagacat caccgaagaa aaaatccggc
    16801 ttaaaaaaca ttgcgactat ttcaaagaga cctttaatac cgaagaaaat tcaggtcgaa
    16861 agttggggtt catttctcag gaaataggcc gggaaattaa tacattgggt tcgaaagcca
    16921 acaattccga cattcaaatt attgttgtac aaatgaaaga tgaactcgaa aaagtgaagg
    16981 agcaattgct gaatgttttg taatgacaaa tatccatgac cgctgaattt attttttatc
    17041 tgatacttac aatacttatc gccggatttt tgattgatat gtttttatcg tatctgaact
    17101 atagaagctc caggcaaccc attcctcaat tactcgatgg gatttacgat aataataaat
    17161 atttggaaca acaagcttac atgaaagcaa accaccgttt tgggaacgtg aattccctgt
    17221 tcagtttgat tgttattctg ctgatgctga ttttcgatgg tttcagctat gccgataatc
    17281 tggcaagaac aatcggagtc agcgaggttt ttacaagcct aatattcttt gctttactcg
    17341 cttttgcaaa cgaaatactc agcattcctt tcgatgttta cgacaccttt gtaattgaga
    17401 agaagttcgg atttaacaaa accagcccca aaatttatat taccgacaaa attaagtcat
    17461 ggcttctgat ggccataatc ggaggcggac tgattacact cattacctgg gtatattatt
    17521 acacggcaga atatttctgg gttttaacat gggtggctat ttttttattc agtattgttg
    17581 ccaattttct gtattccgat ttaattgtac ctcttttcaa taaacagaaa ccgctcgaag
    17641 agggtgaatt gcgcactgca atcagtgact ttgccgccaa agcaggtttt tcattgaaaa
    17701 aaatcatggt aatcgatggc tcaaaacgca gcacaaaagc aaatgcatat ttcagtggat
    17761 tcgggtcgcg caaaagggtt gttttatacg acaccctcat tgccgaaatg cagaccgatg
    17821 aaattgttgc cgttttggcc catgaaatag ggcactataa gaaaaaacac gttctgatta
    17881 atttgattgt ttcatcggtt tcaactgcct gcgtgcttta cttgttatca ctcttcctaa
    17941 aatttcccga gttctcacag gcaatcaaca tcgaaaatgc aagttttcat tcgggactgt
    18001 tggtttttgg aattctgttt acccctattt cagccttagc cgggatattc accaatatga
    18061 gcagccgacg acacgaattt tcagccgaca agtatgcaac aatgcaatac catgcaaatt
    18121 cccttatcag tgcattgaaa aaacttagct ctcacaacat ggtgaatctc acaccgcatc
    18181 catggtttgt ttttgttaat tactcccacc ctccgctatt gcaccgaata aaaagaatta
    18241 atgaaataga agcaaaaagt tgaaaaaata tttatttcgt tattgtcgtt atcggttaaa
    18301 aaattaaatt tgcacaaaaa tcattcaaag aaatggacga ttatcacgaa gcgggcaatt
    18361 attaatcaac cttgttgaag tggtaatcca tcccttcggc aagctaaaat gcaaggaggg
    18421 ttaaccaatg atttcgtact ggcaaattat tacaaacggt atcacaccta tttcggaatt
    18481 taccaaggaa tgtattgtta atgttgtaaa cccgtcaacg gcggagttca atattcttac
    18541 cactactttc ggccttcctg aagaccttat cgctgacgta cttgaccttg acgaacggtc
    18601 cagaatcgag tttgaggatg actttgtttt tatgattatg agaattcccg taataaacaa
    18661 tgaaagcggg attcctttca ataccatccc catgggtgtt ttcatcaaag gtcagaacat
    18721 atttgtgctt tgccaaaaag aaaatcaggt aatcaatggc tttttcaaca agcaaatgcg
    18781 gcgcaaattc tttccacaga ataaggttga ccttgtgctt tatttatttc ttcatgccaa
    18841 taacctgtat catcactacc tgcgtgaaat caatgcccaa accaatctgg ttgaaaaaga
    18901 cctggaacgt tccatacgta acaaagaact tcagggatta ctgaagcttg aaaaatgcct
    18961 tgtatatttt acaacttctt tgcggtcgaa cgaatacatt ctcgctaaat taaaaaattc
    19021 aagttttatc agaaacaacg atttctcaga agacttgctc gaagacgtta taattgagaa
    19081 caaacaggct atcgaaatgt cgaacatata cagcgacatt caaagcggaa tgatggatgc
    19141 ttttgcctca gttatctcga acaacctgaa cattgtaatg aagcgactca cagccatttc
    19201 cattctgctt atgatcccca cattgatagc cagcttgttt ggaatgaatg taccgaattt
    19261 catggaagac tcaaatcttg ccctggtcgg tattttgttg ttttcggtac ttttgtcgat
    19321 aacaggctta ctgattttca gaaagaaaga ctggttttga tactaaaatg cttctttttt
    19381 tctcataaaa atcaggtaaa aaccctacta ataatgcaac tctttatagg ctatttacgt
    19441 ctttaaaata aagagaatgt gttattaaca tcttaaattc ttacgtcatg aaaaattttg
    19501 atagctttcc attgttaaag atgagtctta actatgcgtt aggagttatc gatttctcgg
    19561 gcgaattgga agatgctcga aaaaaagtaa tagccaaaaa gctccttgag gctggctttg
    19621 caataagttc aaacctgtat caggcccgta aagcggaaag gcagaccgat taccttttta
    19681 gtatgaaaaa tgcattgcgt aacaccgatg agtcgattta ctggcttaaa caatgcctga
    19741 acagtgaaaa ttaccccaaa gaccctgaac taaccatgat ggggcaaatg cttatgaaaa
    19801 aaattgagga aaacctgaga actcaaacac ccgacttcag gaaagacacc taaccagggt
    19861 attaggctgc aaacaactta caggtagtga gaagctgcct gtattttttt attcatccga
    19921 ataaagttac ctacaggaat tactttagca taaaattcac ttatctttac ttcttatttt
    19981 caaaaagaaa aaaatatgaa atatgctgca ttggttattt ttgtgttgtg ttttttatcg
    20041 gtacaatcaa aagaattttc aagacttctg cgatttcctg atgtttacga caatcaggta
    20101 gtatttactt atgccggcga tttatatacg ggtgagttag cgggcggaac cgcacggcgg
    20161 ctcacttcag ggcagggaaa tgagatattt gcccattttt cgcccgatgg cagcatgata
    20221 gcgtttaccg cacaatacga tggcaacacc gaggtatatg taatgccttc ggaaggggga
    20281 actcctttga ggcttaccta cactgccaca ctttcgcggg atgatctgag cgatcgcatg
    20341 ggccctaaca atattgtaat gggatggagc cacgatggta aaaaagttat ataccgcagc
    20401 cggaaagcca cattcaacga cttcgtcgga catttattca tggttccggt agagggtgga
    20461 atgtcggagc aaataccctt ttcaactgga agctggtgtt cgttttcgga tgatggaatg
    20521 atgattgcct tcaacagggt tttcagggaa ttcagaacat ggaaatatta caggggcggt
    20581 atggctgatg atataagaat acacgatttt agaacgcaaa cgacggagaa tattacaaat
    20641 aacgatgctc aggatatttt cccgatgtgg cataaaaatc ttgtttattt tctttctgac
    20701 cgcgaccgca ctatgaatct ttttgcctat gacaccataa ccaaaaaaac atcaaagctt
    20761 accaacttca ccctttacga tattaaattt cccaatatcg gcaacaacgg tattatcttc
    20821 gaaaatggcg gatggttata ctattacagt ttcgagaaaa aaaatgttga gcttctgtat
    20881 atcacggttt cggaggataa cagtggctca agaataagct acaaaaatgc atcagaaaga
    20941 attaactcct ggtcgctatc acccgatgga gtgcgcattg cttttggtgc acgcggcgac
    21001 gtttggtcgg tacctgctga agaaggcatt tcaaggaaca tttcatccac gtcaggggtt
    21061 catgaacgca atgtgcggtg gtcgcccgat gggaaatata ttgcttatat ttcggatgaa
    21121 accggtgaaa ccgaagtatt ttacagcaag gctgacggta gcgaaaaacc agttcaggta
    21181 acaaaagctg ccgacaccta tacctattcc ctgaaatggt cgcccgatag taaaaaaata
    21241 ttgtaccacg ataaaagaat gcgtttgcag tacaccgata tcgtttcagg cagcacagtg
    21301 ctggtttgtg cttcagaggt tacagaaacc ggcgattaca actggtcgcc cgatagtaaa
    21361 tggattgctt acatgaacag tgatacttcg cgtatcgcaa aaatttttct tttcagtacc
    21421 gaaacaaaaa agagccatca aattacttcc gactggtacg attcttacaa tccagtgttc
    21481 agcgactgcg gaaagtatct gttttttgtt tcagatcgtg atttcaaccc catatatagc
    21541 gaaaccgagt ggaatcatgc ctatcaggac agggctaaaa tttattttat tacattgaat
    21601 cgaaacgatt tatcgccatt ggccccaaaa aaccaggagg ttgaagtagc tcctgatgaa
    21661 gtaaaaaata tcaaaaccga taaaggcaaa tcaagagaaa aagagaaaga aaatgaaaac
    21721 agcaacgcag ttaaacccgt agtaattgac cttgaaaaca ttggtcaacg catttcggca
    21781 cttccggttc aagcaggcaa ttattggaat ctgtgcgtat tcgataataa ggtatattac
    21841 aactattcca ggatgaatga atctgcctcg ctaaggttgt ttgacttatc gaaaaaagaa
    21901 gaaaaaaacc ttgggaacta cagcagtttc gaaatatcga taaacagaaa aaaaatggca
    21961 ttgccccaat cgggcaacta ctatataatt gacctaccta cagccaatat caatgtaaac
    22021 caatctgcca gtgtaaattt ggacaatatg tatgtgtggg tggatcttca acaggaatgg
    22081 aatcaaattt tcgaagaaac atggagacaa atgcgcgatt ttttctacga tcccggaatg
    22141 cacggagtta gctgggctga catcaggaag aaatatgaac ctttggttgc atttgtaaac
    22201 aacaggcacg accttaatta catcctcggt gaaatgattg gtgaactgaa tatcgggcat
    22261 gcttacactg gtggaggcga atgtccgcag ccgcaaagag tgaaaaccgg actattggga
    22321 gctgagatta gcaaacacga aagtggctat ttcagaattg acaagattct acttggtgaa
    22381 agctgggtaa gcaactgcag gtcaccactg gtggggcaag gaatggaaat tacagaaggt
    22441 gacttcatta ttgctatcaa cggtaaatac ctgaaggacg ttcccgatat ctacagtttg
    22501 ctcgttggga aagccgatgt acaagtggaa ataacgttca gcagcaaacc cgatggaaaa
    22561 aacacacgga aatatataat cgtacccgtt gatagcgaag ataagctcta ttattacact
    22621 tgggtgcagc ataacatcga ctatgtgaac aaagctacca acggacaagt tgggtacatt
    22681 catgtacctg atatgggtca ggaaggcctg aacgaatttg taaaatactt ctatccgcaa
    22741 cttgcaaaaa aagcaattat tatcgatgat cgcgggaatg gcggaggcaa tgtgtcgccc
    22801 atgctcattg agcgccttag acgtgaagtt tcgatgatgg ccatgggtag aaacgctgat
    22861 ataccttata ccaaaccgcg cgaactcctg aacggaccca aggtattact gataaaccaa
    22921 tattcagcct ccgacggtga tttattcccg taccaattta aaaagcacaa gctcgggaaa
    22981 gtaatcggag tgcgcacatg gggtggtgtt gtcggtatcc gaggatcgtt gccttttatt
    23041 gatggtggac tgttgatgaa acccgaattt gcgcactatt ctgccgaagg aaaaggttgg
    23101 ataattgaag gatatggcgt agatcctgac atttatattg acaatgatcc gtatctggaa
    23161 tttaccggcg ttgatactca actcgacaaa gctattgaag tagcccttga ggagttaaaa
    23221 aacaacaatt ataatttgcc cggcatacca gaattcccgg ataagtcgcg ataatatgat
    23281 tgttctgatt tcttaagttt ttattttaat ttgaatattg ttttgtaaga ttttatattt
    23341 ttacagcgaa ttttaacgtt taatcaatta aattataatt atgaaaaatt tcatgtatct
    23401 tttcttagca ctaagcatca gcattgctgt tgtatcatgc ggaaataaga aagacaaaga
    23461 agaaaccaaa aaagcagaaa atctggctga tcttaaaacg aagtacgatg gtaaacaatt
    23521 caaggattgc gacgagtatt ttgccgctgc cgatgaaatg ctggatgtaa tggttgctac
    23581 agttaaaaaa gcggatgccg gcgacgaaaa agcaaaagct gagtttaaag atctggtttc
    23641 atttcttggt cagttcgaaa ccgagggtga aaaatataaa actgaatgcc ctgataaatt
    23701 caaagaattc gaaaagaaat ctgatgaaaa agcaaagcct attgaagagg ccattatgaa
    23761 gcttacattc gatatgatgc aggatgaaat gccttctgta gaagacagcc tgcaagccat
    23821 ttcgaatgaa gttgaaaaca agatcgaagg cattcaaacc gaaggcaaca acaaactgga
    23881 tgcgctgaag aaaaagaaat aaacttaaca atactgaaga aagcaggtct taagcctgct
    23941 ttctttgttt tataattaaa acattttcga aacaaagctg ttaaaacttt acatgaaaag
    24001 ctttttaagt aagaacaaat tttattagat ttaccgtttt ataaaactta aaaaaatggc
    24061 agatatttct acggtttata tgggcatgaa gc
//
LOCUS       DAOOQP010000033        23942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2575_length_23942_cov_5.540964, whole genome shotgun sequence.
ACCESSION   DAOOQP010000033 DAOOQP010000000
VERSION     DAOOQP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2575_length_23942_cov_5.540964"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLK75_05315"
     CDS             <1..405
                     /locus_tag="PLK75_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOY38701.1"
                     /translation="LAGPDLKVSFMDHKRAVVHIARDAGVKCKEFFGADLPVDMDVLI
                     AGAILADVGKLLEYEMGSDGKSKVGIYGKYLRHPFSGVSLAEACGVPAAVCHIVATHA
                     GEGDMVKRTTEAFLVHHADFMTFEPFKDRLKV"
     gene            408..1640
                     /locus_tag="PLK75_05320"
     CDS             408..1640
                     /locus_tag="PLK75_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOY38702.1"
                     /translation="MDITVITIGDELLIGQTVDTNSAWIGNNLNQFGFAVQCILSVRD
                     VKTDIQNAIDSSFGLTDVIIMTGGLGPTNDDITKNVLCDYFGTELVLHHESLELIKSF
                     VEKRGYPLTETNRNQAMVPAACTPLLNRMGTAPGMWFSRGKKILISLPGVPYEMKDLM
                     EKEVIPRLISCYKLPPVEHRYIQTHGIPEAFLADKLRCFENCLPSSLKLAFLPSQGII
                     KLRLSIYEASIQQTQLLDKKYAELCSIIPEYIIALNDESFQMNIGQLLLQRNKKLSLA
                     ESCTGGYISHLFTQIPGCSAWYQGGVVAYSNQLKTEILGVDPVTLQQFGAVSVQVAEQ
                     MASGAKQTMHADYSISTTGIAGPDGGTPDKPVGTVCIAMATENGVKSHQYHFGNDRQN
                     NIVRTANTALAMLIREIK"
     gene            1683..2426
                     /locus_tag="PLK75_05325"
     CDS             1683..2426
                     /locus_tag="PLK75_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY38703.1"
                     /translation="MIKEIAQHRSIRKFKPEPISDTILNEILEAATRASTTGNMQVYS
                     IIVTTDNEIKERLLPCHFNQAMVQQAPVVMTFCADFNRFTRWCRLRNADDGAYNNFLS
                     FVTAAIDALLAAQNASLEAEAHGLGICYLGTTTYTASKIIEILNIPKGVVPVTTLVIG
                     YPAELPALTDRLPMRAVVHSQTYQDYSDMEIEALYIEKESRQDSRKFVAENNKQNLAQ
                     VFTDVRYTRKDNILFSDMLFNVLKVQGFL"
     gene            2589..7070
                     /locus_tag="PLK75_05330"
     CDS             2589..7070
                     /locus_tag="PLK75_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY38704.1"
                     /translation="MRKFLLSLTLGITVIAYVQAQSTTHFELTQALISNNTRCISQDS
                     DGNIWVGTTAGITRYDGFSWTSFTTEDGLGNNLIYDIYCSSDGDVWAATAGGISVYDG
                     SNWTIYNLGSGLPSNTVWCISGDAAGNIWGGTSNAGAFKFDGSVFTIFSTSQGLISNA
                     VKTIYGDRYGNVWMGTASGLSKYDGESFRSFTTANGLGGNLINDITQLSNGSIAVATN
                     GGLSQYNFATWHTITTAQGLPNNNVLCVSENDENILYLGSSLGLFEYESPASYHLYTI
                     SDGLTANVVTSVFIDQSGSVWAGSPFSGLTRYNRGNQSLITRFNKDIVDNHVNHIYRD
                     NQGKFWIATNAGLNCVDDYKWRTYTSTEGLVSNEITCTYKDANNVVWIGTTAGITRME
                     GTTMTQITTSNGLTHDHVNAITSDASGLIYVATDEKVSVIQGSAVIDEISTDDGLASN
                     LIYMVFYDIDGRLWILCDGGITYREGDTFYDADVLPAFPVGRPVSIANNPLGGVMIGS
                     DENLYFYQNGTTGASAVVHPLAATNQAINSMSSLSGLIYCSFESGELYSYNLSSWTSI
                     AVANPVSYCYFDPWGYTWIGYQEDGLDKICRTCNTNLTYTLSAFACGSGNTPVTTLTI
                     TSPAGVIYTVNGGIENSNNPVFNNLVPSAYHLRVYNAGAYIADSIIFIEGHGGIETYL
                     TLGQIDCNGNNNGWIEMHDLPSPGSFIWNTGNNSQLLMENLVPDMYQVTVSDNIGCTK
                     IIQNTIQQPNVLQVGITSTQVLCYGNATGSITLSPTGGTPPYSIEWSDSYTGFSLTNL
                     PAGMYAYTVTDINGCEASGNVTITQPAAALQLSGTGEAVACYGDTGDITTSVTGGTLP
                     YTYHWNDNFSGANRTNMPAGTYYLTVSDVNNCSTTGSWQITQPIAALEISDVDEVDVY
                     CFGNSSGSIDVTIEGGTSPYEYSWTKNSVFYSTDEDLENLSIGVYQLVVTDDHGCADG
                     VSVTISQSPPLQLDIDATPITCPGYANGVLYADVSGGSETYSSFYWYNSQNQVVSVDQ
                     TFEDVAPGYYFLSVTDSYYCTITDEVTVTEGDGHDITLQITDATCYGAASGSIHVVVD
                     GGATTDVDYAWAAGVAGNTATATNLEAGEYFVTITDNEGCETSANGIVNQPPMADLGA
                     FPANGEFRFCSGDQLLLDAGDGFTSYLWSTGSQNQTIPVNNGGSYSVLVHNSTGCILG
                     DTATVVVGEVFQDEELNLASVNEQNKVTLYWQKTPDVGTDHYNIYRKSGSQYVLYDDL
                     DADLPAIYVDENSNASENTFSYKISSVDTCGNESALSDAHSSMYLLAQSEEYNQQVVC
                     TLSYTPYEGFFVVYYYILKGTSPDNLEVADQNLYNEFEYIEFNPNPEGTYYQIMVRRL
                     DGCYPGDGNYYDTAYSNIVFCQPYNGIANRNIDNQVTVYPTIFDDYISLNFNLQQASD
                     VSFYFINSLGQAIVLEEKHRVDVGNQQIDLHPNLAPGMYMLKMKIGNEWITRKVIKN"
     gene            complement(7171..8274)
                     /locus_tag="PLK75_05335"
     CDS             complement(7171..8274)
                     /locus_tag="PLK75_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOY38705.1"
                     /translation="MNKNRFSLTRSTSKQGYDWWWHSFVAENANTGELKPFFIEYYVI
                     NPGLFAGDIVWGQKGSSENPSPKPCYAMIKAGTWGAPKAQLHDFYKISDFEASTKELN
                     CRIGNNEVTEKSLKGQVVVTTQETIDFPERMSDAGTMQWDLSVEKHLSYDVGYGSSHL
                     LNALNAFEMFWHVGGMRCSYRGTVTFDGVTYIVKPETSYGYQDKNWGKNYTNPWIWLN
                     CNHFVSKKTGEPVDASLDIGGGCPKVFGISLKRRIITALYYNNNLIEFNFSKFWKFSS
                     QQFSIREDDDKFYWNVVSTNRQHKIEVDFSCEKSTMLFVNYESPDGKKLHNKLWNGGH
                     AKGSVKIYRKQQGEFHLIEELDGSMGGCEYGEY"
     gene            complement(8387..10285)
                     /locus_tag="PLK75_05340"
     CDS             complement(8387..10285)
                     /locus_tag="PLK75_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOY38706.1"
                     /translation="MNLTRIFDILELYKTDFSSADDALSYKFKDNWINFSAYDYHHYS
                     YLFACGLIAGGYQKGDKIATISQNMPHWNIADMGISMAGLVHIPIYPTLSAKETEFIL
                     RHSDSKLLFISDKNIYRKLQPVLSNIESIEAVYTFNDVEDAENWVYFIQKGKDEEERI
                     KSILEQRSAEISPDDLHTIIYTSGTTGNPKGVMLTHRNILSNALAVYDILPVQFGHKA
                     LSFLPLCHVYERMVNYSFQIKGIRIYYAESMGTIVDNLKEVKPHIFNSVPRLLEKFYD
                     KIIAIGKDLRGIKRHIFFWAIRLGVKYKSDGNNSAFYNAKLKLARKLVFSKWQAAFGG
                     NVIAVVSGGSSLQERLNKLFHAAGVPICEGYGLSETSPVLAVNWPDFKNKRIGTVGPV
                     LEGVTIKIADDGEILAKGVNVMKGYYKNPEETAKVFDEEGWFHTGDIGTWIEGRFLKI
                     TDRKKEIFKTSSGKYVAPQVIENMFKASSLIEQIMIVGENEKFVSALISPNFNYLHFW
                     ATKHKIHYRDNPELIRQPQVIQKIQHEIDMVNKTLGEHEKIKRFRLVHEEWSPASGEL
                     SPTLKLKRNVIYAKYENILSEIYGHGVDKTEKMGKETGGDSIDKIIANGLSKLMNITI
                     TGKDKDKE"
     gene            complement(10307..10951)
                     /locus_tag="PLK75_05345"
     CDS             complement(10307..10951)
                     /locus_tag="PLK75_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38707.1"
                     /translation="MNISKKIVLLGFVFAFSGIIHAQTMIHPKVGIIGVHPFSTPNRH
                     LFENRIDSEAWFVTEPLLMVSIESLIKSDYFSWRIMPGFYSDAASKPGFFFNAALKFR
                     LFQSWRNSLHVAVGGSIVGRQLWNTIPGYVYESGFKTNGTWEYRICPFAELEYAFFLH
                     DKGDITCSVLYGHQHETFTFSLGYRIWLSTVIKHPAHCGTCPYEKADGKYKKYK"
     gene            complement(11053..12867)
                     /locus_tag="PLK75_05350"
     CDS             complement(11053..12867)
                     /locus_tag="PLK75_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOY38708.1"
                     /translation="MMSRDILVQNFLPTSKKEIESLGWDYIDVILFTGDAYIDHPAFG
                     IAVIARVLQHESYRVAIIPQPNWQDDLRDFRKLGKPRLFFGVSAGNMDSMVNHYTANK
                     RLRSNDAYTAGDKAGARPDYATVVYSKILKQLYPDTPIVLGGIEASMRRFAHYDYWQN
                     KVMPSILIQSEADYLVYGMGEKPVAMLAKALAAGTNTSEIPQLAYCTGSYENLKGNLL
                     ILNSFEECSTDKKKYAANFVSVETESNKMNPQMLVQKTGNVYVVVNPHLPPISAEELD
                     NIYGLPFTRLPHPRYRNKPPIPAYDMIKHSVTIHRGCFGGCSFCTISAHQGKFVSSRS
                     KNSIIDEVKRIVAMPDFSGHISDLGGPSANMYNMNGENLQLCEKCSRPSCIFPAICKN
                     LNISHQPLIDLYKSVAEQNGINHVSIGSGVRYDIPLFRSKNEEVNRANRNYLKLLIEK
                     YISGRLKVAPEHTSEKVLKLMRKPSYKDFIQLKQLFDTMNSKGQRRLQLIPYFISSHP
                     GCTISDMAELAAITADQGYKLEQVQDFTPTPMTLATVMYYTGIDPYTGNRIPVAFTVD
                     DKKGQTKYFFWWKPENKELILKELRKLGLMNYAKALFK"
     gene            complement(12867..13505)
                     /locus_tag="PLK75_05355"
     CDS             complement(12867..13505)
                     /locus_tag="PLK75_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38709.1"
                     /translation="MKSLVLSFGLLILIAGYVNAQEENYYVSPDSSVNLLQYDAGTPP
                     FSSGKRLNMGLNMGSSLGSFYGNTLFTNYISPEIRYSFTPKFSLTAGTMFTYSMYGNS
                     VFGDDSGLESEASKQARFYLYARGEYQVSDRLWVRAGGFAEVTPGASQYGYKAGHVGM
                     TFKISEETWFNADFQINSGYPGTDLYYSNGGIFGDPTRFGNYEANPYSGLYW"
     gene            complement(13636..13735)
                     /gene="ffs"
                     /locus_tag="PLK75_05360"
     ncRNA           complement(13636..13735)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLK75_05360"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13824..14411
                     /locus_tag="PLK75_05365"
     CDS             13824..14411
                     /locus_tag="PLK75_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOY38710.1"
                     /translation="MKNNLKVILRYGLILGLVNIAFSVLVYVFDVVLMGMFAGFFISL
                     ISLLIIIIALVWSGKHYRNNYNGGYIKYTQALLLAVMIVAVAALVVFCYDVVFRTAIE
                     PGYDERVKTEITQKTVQYMEEKGVPDGEIDKVIDKMNEQKPKPLAIKLVWSFFGPLIF
                     GVIIALITSIFVKKNKPLFDGAEETPDSTGSQEVM"
     gene            14411..15358
                     /locus_tag="PLK75_05370"
     CDS             14411..15358
                     /locus_tag="PLK75_05370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY38711.1"
                     /translation="MNISVIIPLYNESESLPELMQWIAKVMSQHDYSYEVIMVDDGSN
                     DNSWEVIENLSLQYPALKAIKFRRNYGKSPALQTGFQAAHGDVVITMDADLQDSPDEI
                     PELYRMIVEGKFDLVSGWKQKRHDPISKRWPSKFFNQTVRVTTGIKLHDFNCGLKAYR
                     QSVVKSIEVFGEMHRYIPVLAKRAGFKKIGEKVVQHQARKFGYSKFGLERYMNGYLDL
                     LTVLFITRFGKKPMHIFGVLGSLVFLLGFVAAAYLGIHKLIAVHNGIHIERVTSSPYF
                     YLSLTCIVLGSMLFMTGFLGELISRSASDRNHYLIEKEI"
     gene            15372..16175
                     /locus_tag="PLK75_05375"
     CDS             15372..16175
                     /locus_tag="PLK75_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38712.1"
                     /translation="MAKEREIDLVELYIKIVSFLKRRYLLLIIITVIGVGLGLARHFT
                     APPRYRVIMIASSEILDKQYVYALASPMSMYVEQRKYVDLSNYLGCDVKALSAVKALE
                     LDSTLLNSVKLEMIFSDSSSIKAFRDAVINFYNKQKFIVAYYENEKKNLNDFVKTIDS
                     EIAELNEFQAKMLNQENSKNNIEIYTSIAGSHEEMITLYEKRYELRGKMMQTQPISFI
                     NDGLVMEKNNSIALGLLIWGGVFFILALITGIYLDLKLAARNRRNSKLV"
     gene            complement(16161..17423)
                     /locus_tag="PLK75_05380"
     CDS             complement(16161..17423)
                     /locus_tag="PLK75_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY38713.1"
                     /translation="MYSSVNNTSQVKKIVLVNHYAGSPEMGMEFRPYYMALLWQKMGY
                     EVMIVASSYSHLRSKQPVVTNNGLHEIEGVSYALFKTKPYCGNGLKRILNIFEFVRHL
                     YTRAAKVITDFNPDVIIASSTYPLEIYPLKKLARKNNAKLIFEVHDLWPLSPRLLGGY
                     SKLHPFIYIMQQAENYAYRNSDAVVSLLPCTLEHMQEHGLKKEKWYYVPNGIYLYDDA
                     EQEIELPQAHNELIHRLKKENALLVGYAGSIGVANNIRMLIDSRRLIQNDRIHLIITG
                     NGPEKEKIIQQMSDSDRKYVHFLDAVPKRAVVTLLRKMDMLIITWNKSPLYAYGVSAN
                     KLFDYMFSAKPIVQALEAGNDPVMESGCGITVEPENKTRIAEAIEAIANMPEEERIKM
                     GEKGKQFVLKKHIYQVLAKEFAEILHKL"
     gene            complement(17498..21226)
                     /locus_tag="PLK75_05385"
     CDS             complement(17498..21226)
                     /locus_tag="PLK75_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38714.1"
                     /translation="MKRLFQTFFACIILLLATVQVKSQYLGGYEPSPGEQAYLDSLPG
                     PYISFYSKNLQLPPVVDNSLKPYFRSIFSQDGGSCVQASTIGYVFTYMVNRERNISSN
                     SNETTYPTHYTWNYCNNATNNGSGISTGWNVIKKSGIPNIASYGGLYRTFADDDANFT
                     KRRTVWMSGYSDYRLALNNRVIESVFSIELMTSDDLDDIKHYLHDLGIGDLVKGGGLL
                     SFGENFQNPVINDLVYPSAEAGKKVVTQWPTSGFGHAMTIVGYNDNVMWDFNGDGLYT
                     NDGIISNWEIGAFKVANSWGAGWANDGFVYIPYRLFYSIYLSGIVIAENTVYNPKVTL
                     RIEMDYSQRTYVNPRTGVSTDSASTTPQFIEQYETYWMPSGHIPMQGINEYPIEIELD
                     ITRKLSNIIPAKFFLDLHLVQNPGAGEVLNVSVVDYDTKDGIPVVVNCLPSFVPHTIT
                     QGFNRIPVDYDYLPSVIKQEIVVNRDLYIQKDISIADGGTLVINNSNIQLLENKSIVV
                     NPGGTLEILNGGSVTVSGNGSIQIESHTTNPAVLVYYNGATIDLVDNNTCLDISGNLS
                     IAANAQFTFTGAGYIKFSNPGDMYTENITCGTGASMLFQGSGKSDKVVEVQQDAVRFP
                     ALESLTFEDCKIVMSAGDQMLATADYPITFDNVLMTSATGINGKHRGFCFYGQENTVV
                     NNCIFEYSSYGITGDLSVYGSPLEVSNSEFRYNTNGIHIYGRGIHASFCNFNHCNSNG
                     AYCYGMSLPSEFSNCEFHHNGHNGFTFYGSSAADLDVEACDIYSNIYDGIITSGAFDL
                     RLSCNNIYANRYGVYTQNGTYVYAENNAENDIHSNVRSIYLNYGRLFLNEGYNQLQAA
                     TDAYAVYGFSVEPGNCSASPQNIANNNRWEADDTEGPDYGTNYKLWKFQCSNPLVLVQ
                     LVDSIPDYTVCYHIDPDSLQEGGSCFQSNEETANLLNSLPLISLSGGISLPLDQAVTY
                     LANQSDGVSSANAFNNLIDEYANLIFQCDNNTSDAVFLLKNKAYGDVHNTLTNYYEFI
                     DYNSGHNGFNQAVDRMVLLHEDLLINPDIPDYLSIEYKLDIAMLNYLRKDYNRSITQL
                     EFLINEIPDTVNAKAFVENWLCFVSAEKALREGTILPEEYEQSIAECNINYPVTIFDS
                     PDHDEVQNANSTSEETAQVSIAINPNPNQGSFTVLVTNSSISGKIMITKATGQPVYET
                     ILTFAEQLVAVNGLPAGTFTVYYIENGTVITSVGTIVE"
     gene            complement(21267..22487)
                     /locus_tag="PLK75_05390"
     CDS             complement(21267..22487)
                     /locus_tag="PLK75_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38715.1"
                     /translation="MRIIAMIGLFIGVIGQINAQNFWTEIERPIDKPVYAMAVTNTNT
                     VYLGMGMDSGVYGSLTENYNWDLHELDGLSVYSLHIDLNERIFAGTGGFNSIYNFDIN
                     EQLWTPIFSGVGNVLSIFSNMDYLLIGFWGGIFKTNDLEDWEQTLDVDNDQVVNSICS
                     DHNNVLYAGITSFMTDAGGVYRSFDQGETWEYAELSGYYIKSLACDSNGNLYAGSTGN
                     YGIGIYRSQDQGLTWTGLKNDVFVESIVITPDDHIYIGCSNEFGTQGGVFVSTDYGET
                     WEMINIGLNNENVYGLCYAPNGYLYAYGNHLHRSQNSVYTKIETQLISNQVFMVFPNP
                     VADYLQIKSQTKPRSTGSVTIKIVDMYGRLICSENNYISNSRIDVSGLDDGVYLLLIE
                     AENKRFEQRIVKIK"
     gene            complement(22513..>22804)
                     /locus_tag="PLK75_05395"
     CDS             complement(22513..>22804)
                     /locus_tag="PLK75_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38716.1"
                     /translation="PDHDEVQNANSTSEETAQISIAINPNPNQGSFTVLVTNSSISGK
                     IMITNATGQPVYETILTFAEQLVTVNGLPAGTFTVYYIENGTVITSVGVIVE"
     assembly_gap    22805..22904
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22905..>23942)
                     /locus_tag="PLK75_05400"
     CDS             complement(<22905..>23942)
                     /locus_tag="PLK75_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY38717.1"
                     /translation="LLSQSGARVINASWIDRCAYSNIDWLVFEEIKDNGTITVCGAGN
                     SIAHCGSLDAEVFPASYDNCISVTSVGHMNPIGYTDSIYGQTNWKDVHEAVIGDPGTA
                     HHHNTAVDICAPGYNVPSTSIGNTYQGTWGTSFASPQVAGICALILSVNPCLSRDEAV
                     DILLSTAVDIDDIPENIAYNELLGSGRADAFEAMKKAYGNSETIYSGETIVWNSFKSI
                     NGTLLLQANSHLTINTRVYFNENSNIVVKNGASLVLESDGYLTSFCDKTWSVIIVEPG
                     GIVELRNESIVNINGNGCIYVGKDTNYSSVLIFNQGAIINLVDNESYIEIKGNLSIAA
                     NAQFTFTGAGY"
BASE COUNT         6970 a         4953 c         4678 g         7241 t
ORIGIN      
        1 cttgcaggtc ccgatctgaa agtaagtttt atggaccata aacgtgcggt tgtgcatatt
       61 gcccgtgatg ccggagttaa atgcaaagag ttttttggcg ctgatttacc tgttgacatg
      121 gacgtactga ttgcgggagc tattcttgct gacgtaggga aacttcttga gtatgaaatg
      181 ggttctgatg gaaaaagtaa agtcggtatt tacggaaaat acctccgtca cccatttagc
      241 ggggtttcgt tagcagaagc atgcggtgtg ccggcagcag tatgtcatat cgttgcaact
      301 catgccggtg aaggtgacat ggtaaagcgt accaccgaag cgtttttagt tcatcatgcc
      361 gattttatga catttgagcc tttcaaagac cggctgaaag tataacaatg gatattacag
      421 ttattactat cggggatgaa ctgctgatag ggcaaactgt tgatacaaac tcggcatgga
      481 tagggaataa cctgaatcaa ttcgggtttg cagtgcaatg tatcctttcg gtgcgtgatg
      541 ttaaaaccga tatccaaaat gccatcgatt catcatttgg gttaaccgat gttattatca
      601 tgaccggagg cctaggtcca accaatgatg atattacaaa aaatgtgttg tgcgactatt
      661 tcgggacaga attggtatta catcatgaat cattagagct tattaaaagt tttgttgaaa
      721 aacgtggcta tccattaaca gagacaaacc gaaatcaggc aatggtacct gctgcttgta
      781 ctccattgct taaccgaatg ggtactgcgc cggggatgtg gttcagcaga ggaaaaaaaa
      841 ttcttatttc tctgcccgga gtaccatacg aaatgaaaga ccttatggag aaagaagtta
      901 ttccaaggct tatttcatgt tataagttgc ctcctgttga gcatcggtat atccaaacac
      961 acggcattcc cgaggctttt cttgcggaca aactgaggtg ctttgaaaat tgtctgccat
     1021 cctccctcaa attggccttt cttccatctc agggtattat caagcttagg ttgagcattt
     1081 acgaagcaag tatacaacag acacaattgc tcgataaaaa atatgcagaa ttatgttcaa
     1141 ttattccgga atatattatt gcgttgaatg atgaaagttt tcagatgaat attgggcaat
     1201 tgctcctgca acgaaacaaa aaactttcgt tagccgaaag ctgcacgggc gggtacatct
     1261 ctcatttatt tacacaaata cccggatgtt cggcctggta tcaggggggg gttgttgcat
     1321 actcaaatca gttaaaaaca gaaatactag gggttgatcc tgtaacactg caacagtttg
     1381 gtgcagtaag cgtccaggtt gctgagcaaa tggcatcggg tgcaaaacaa accatgcatg
     1441 cagactatag catttcaacc acaggaatag ccggaccaga cggtggaact cctgataaac
     1501 ccgtaggtac agtttgcatt gcgatggcta ccgagaatgg agtaaaatcg caccaatatc
     1561 atttcggtaa cgaccggcaa aacaacattg tgcgcactgc caatacagcc cttgcaatgc
     1621 tgatcagaga aataaaataa accatacagc ttccaattta ttaataaata accgataaaa
     1681 tgatgataaa agaaattgcc caacatcgtt caattagaaa gttcaaacca gagcccatta
     1741 gtgatacaat actgaatgaa attttggaag cagcaacgcg ggcaagtacc accggcaata
     1801 tgcaggttta cagtataatt gttaccactg ataatgaaat taaggagcgt cttttacctt
     1861 gccattttaa tcaggcgatg gttcagcagg ctccggttgt aatgactttt tgtgctgatt
     1921 tcaaccgttt cacacgttgg tgccgcttaa gaaatgccga tgacggtgca tataacaatt
     1981 ttttatcgtt tgtaaccgcc gcgatagacg cactactcgc agcacaaaat gccagccttg
     2041 aagcagaagc tcatggttta ggaatttgtt atctgggaac tacaacttat actgcatcga
     2101 aaatcattga aatactcaat attcccaaag gggtggtacc tgtaaccact cttgttatag
     2161 ggtatcccgc cgaattacct gcgcttaccg acagacttcc catgagagct gtagttcact
     2221 ctcaaaccta tcaggactac tcagatatgg aaattgaggc gttgtatatt gaaaaagaat
     2281 cgcgccagga cagcaggaaa tttgtcgctg aaaataataa acaaaatttg gctcaggttt
     2341 ttactgatgt aaggtacaca cgaaaagaca atatattatt ttcggacatg ctcttcaatg
     2401 ttcttaaagt tcaagggttt ctgtaatact aacgaattta cttcattcag aggtcttctg
     2461 ataaatatta atacatgcgg tttataaatt cttggacaca gtatgagata attttgtgtt
     2521 tcaattagct gtttgctatc tttaggcatt gaataatacc atttataaac taacaaatga
     2581 caccaatcat gagaaaattt ttattatcgc tcacattggg aattactgta attgcttacg
     2641 tgcaagcaca atcaaccacg cacttcgaac ttacacaggc acttatatca aacaacacca
     2701 ggtgcatatc tcaggattcc gacggcaata tatgggttgg aacaactgca gggataacac
     2761 gatacgacgg cttttcctgg acaagcttta ccactgaaga cggactgggc aacaacctga
     2821 tatatgatat ttattgttct tccgatggcg acgtatgggc tgcaacagca ggaggaattt
     2881 cggtttatga cggaagtaac tggacaattt ataaccttgg aagcgggcta ccttcgaata
     2941 ctgtttggtg cattagcggt gatgctgccg gaaatatctg gggggggaca agcaatgccg
     3001 gtgcttttaa atttgacggg agtgtgttta caattttcag cacttcgcag ggactcatca
     3061 gcaatgcagt aaaaaccata tatggcgacc gttacggcaa tgtttggatg ggaacagctt
     3121 cgggactcag caaatatgat ggcgaaagtt ttcgaagttt tactacagcc aacggactgg
     3181 gaggaaatct cataaacgat ataactcaac ttagcaatgg aagcatagca gtagccacaa
     3241 atggaggact cagccagtat aactttgcca catggcatac cattacaact gcacagggct
     3301 tacccaacaa taatgttctt tgtgtttcgg agaacgacga aaatatattg tatttggggt
     3361 cgagccttgg cttgttcgaa tatgagagcc ctgccagtta tcatctgtat actataagtg
     3421 acggattaac tgccaatgtg gtaaccagtg tttttattga tcaaagcggt tcagtctggg
     3481 caggatcacc cttttcgggt ttgacccgct acaatcgagg aaatcaaagc cttattacac
     3541 gcttcaacaa agatatcgtc gacaatcacg taaatcatat ttatagggac aatcaaggga
     3601 aattctggat tgccaccaat gccgggctaa attgtgtaga cgactataaa tggagaacct
     3661 atacaagtac cgagggattg gtatcgaacg aaattacctg tacttacaaa gacgctaata
     3721 atgttgtatg gattggtact acagcgggga ttacccgaat ggagggtaca acaatgactc
     3781 aaataacaac ttcaaacggc ctgacacacg accatgtgaa tgcaattact tcagatgctt
     3841 caggtttgat ttatgtggcc accgacgaaa aagtttccgt tattcagggc tcagctgtaa
     3901 tcgatgaaat aagcactgat gatggtttgg catcgaattt aatctatatg gtattttacg
     3961 atattgatgg taggttatgg attttgtgtg atggtggaat tacctatcgt gagggtgata
     4021 ctttttatga tgctgacgtg cttccggcat ttccggtggg gcgacccgta tcgattgcca
     4081 ataacccatt aggaggagta atgattggct ctgatgaaaa cctttatttt tatcagaatg
     4141 gaaccaccgg agccagtgct gtagtgcatc cattggctgc taccaatcag gctataaatt
     4201 ccatgtcatc tttgtcgggc ttgatatatt gtagttttga atccggtgag ctttactcat
     4261 ataacctttc gtcgtggact tcaattgctg ttgcaaaccc tgtgtcgtat tgttattttg
     4321 acccttgggg ctatacatgg attggatatc aggaagatgg actcgacaag atttgccgga
     4381 catgtaatac aaatctcacc tatactttgt cggcatttgc ctgtggttcg ggtaatacac
     4441 cggttactac cctgacaatt acatcgcctg ccggtgtaat ttatacggtc aatggtggta
     4501 ttgaaaattc aaataacccg gtgtttaaca atctcgttcc gagtgcttat cacctaagag
     4561 tatataatgc cggagcttat attgctgata gcattatttt cattgaaggc catggaggta
     4621 ttgaaactta tctgaccttg ggtcagattg attgcaacgg caacaacaac gggtggattg
     4681 aaatgcatga tcttccatcg cccggtagtt ttatttggaa tacaggaaat aactcgcaac
     4741 ttttaatgga aaatctggtc ccagacatgt atcaagttac tgtttccgat aatataggat
     4801 gtacaaaaat tattcaaaac accattcaac aacccaatgt acttcaggta ggtattacct
     4861 caactcaggt attatgttat gggaatgcca caggcagtat caccctttca ccaacaggcg
     4921 gaactccacc ctactcaatt gagtggtccg attcctatac agggttttct ttaaccaatc
     4981 ttccagccgg catgtatgca tatactgtta cagatataaa tgggtgtgaa gcatcaggaa
     5041 atgtaactat tacgcaacca gctgcggcac ttcaattatc aggcaccggt gaagctgtgg
     5101 cttgctatgg agatactggc gatattacaa catcagttac cggtggaacc ttgccatata
     5161 cctatcattg gaacgataat ttttcggggg ctaaccgtac caatatgcca gcaggcactt
     5221 actacctaac tgtttcggat gtgaacaatt gcagcactac cggttcctgg caaattacac
     5281 agccgattgc tgcacttgag atcagcgatg ttgacgaagt ggatgtatac tgctttggaa
     5341 actcctcggg ttcaattgac gttaccattg aagggggtac cagcccttat gaatactcgt
     5401 ggacaaaaaa ctctgttttt tattctactg atgaagatct tgaaaatctc tctattggtg
     5461 tttatcaatt agtcgtaacc gatgaccatg gatgtgctga tggtgtttct gtaacaatat
     5521 cgcaatcgcc gccactacag cttgacattg atgctacacc aattacttgc cccggatatg
     5581 ccaacggagt tttatatgcc gatgtatccg gaggctcaga aacttacagt agtttttact
     5641 ggtacaatag ccaaaatcag gttgtaagtg ttgatcagac ttttgaagat gttgcgccgg
     5701 gatactattt cctttcggta accgatagtt actattgcac cattaccgat gaagttactg
     5761 ttaccgaagg cgacggacat gacattacat tgcagattac cgatgcaaca tgttatggtg
     5821 ctgcaagcgg gagcattcat gttgttgttg atggcggagc aaccactgat gtagattatg
     5881 cgtgggctgc aggtgtggca ggaaatactg ccacagcaac aaatcttgaa gcaggtgaat
     5941 attttgtgac tattactgac aacgaaggct gtgaaacttc ggcaaatggc attgtaaatc
     6001 agcctcctat ggccgatttg ggggcattcc ctgctaatgg agaatttcgt ttctgcagtg
     6061 gcgatcagtt actactagat gccggtgatg gatttacaag ctacctttgg tcaaccggat
     6121 ctcagaatca gacaatcccg gtaaacaacg gaggttccta ctctgtattg gttcataatt
     6181 ccacaggctg cattcttggc gatacagcga ctgttgtggt tggcgaggtg tttcaagatg
     6241 aagaactgaa ccttgcatca gtgaacgaac agaataaagt aaccctgtat tggcaaaaaa
     6301 ctcctgatgt tggaaccgat cattataata tttatcggaa atcaggaagc cagtatgtac
     6361 tgtacgatga cctcgatgct gatctacctg ctatttatgt ggacgagaat tcaaatgcat
     6421 cggaaaacac attcagttat aaaatatctt cagttgatac atgtgggaat gagtcagcct
     6481 tgagcgatgc acattcttca atgtatttgc tggcacaatc ggaagaatac aatcagcaag
     6541 tggtttgtac gctgagttat actccttatg aaggattctt cgttgtttac tattatatat
     6601 tgaagggaac ttcccctgat aatcttgaag tcgctgatca aaatttgtac aacgagtttg
     6661 aatatattga gtttaatccc aatccggaag gaacatacta ccaaatcatg gtgcggcgcc
     6721 tcgacgggtg ctacccaggc gatgggaatt attatgatac tgcatattca aatatagttt
     6781 tctgccagcc ttacaacggc attgcaaaca gaaatattga caatcaggtt accgtgtatc
     6841 cgacaatttt cgacgattat atcagcctga attttaacct tcagcaagcc agcgatgtta
     6901 gcttctattt cattaacagt cttggtcaag ctattgtttt agaggaaaag cacagagtag
     6961 atgttggtaa tcaacagatt gatcttcacc caaacctcgc tcccggtatg tatatgctga
     7021 aaatgaaaat tgggaatgaa tggattacga gaaaagtaat taaaaattaa aacaaagttg
     7081 cacagtgact ttggaaagag taatcctgct attttccact atttgaacca attattgaat
     7141 tagtgtttac aattgattga ataatccgga tcaatactct ccatattcgc atcctcccat
     7201 cgaaccgtcg agttcttcaa taagatgaaa ttctccttgt tgttttcgat agatttttac
     7261 gctaccctta gcatggccac cgttccacaa tttattgtga agttttttgc catcaggtga
     7321 ctcgtaattc acaaacagca tcgtcgattt ttcgcaactg aaatccactt ctatcttgtg
     7381 ctgacggttt gtcgaaacta cgttccagta aaacttatcg tcgtcttccc gaatgctgaa
     7441 ttgctgactc gaaaatttcc agaactttga aaaattgaac tcaatcaaat tgttattgta
     7501 ataaagtgcg gtaataattc ttcgtttcag cgaaataccg aacactttgg ggcatccgcc
     7561 tccgatatcg agactggcat caaccggttc accggttttt ttcgaaacga aatggttgca
     7621 gttaagccaa atccatgggt tggtgtagtt cttcccccaa tttttatcct gataaccgta
     7681 tgatgtttct ggtttcacga tataagttac tccgtcgaaa gtcacagtgc cacggtatga
     7741 gcatctcatt ccgccaacat gccagaacat ttcgaaggca ttcaaggcat tcagcaggtg
     7801 cgaacttccg taacccacat cgtagcttag atgcttttcc acgcttaaat cccattgcat
     7861 cgtccctgca tcgctcatgc gttccgggaa atcgatggtt tcctgtgttg ttactactac
     7921 ctgtccctta aggctttttt cggtaacttc attgttgcct atcctgcaat taagctcttt
     7981 ggttgaggct tcgaaatcag aaattttata aaagtcgtgc aactgcgcct tcggagcacc
     8041 ccatgtccct gcttttatca tagcataaca gggcttcgga ctcggatttt ccgaactgcc
     8101 tttttgaccc cacacaatat cgccggcgaa caaacccgga tttatcacat aatactcaat
     8161 gaaaaacggc ttcaattctc cggtgtttgc attttcagca acaaatgagt gccaccacca
     8221 gtcgtatcct tgtttcgagg tacttcgtgt gaggctgaat ctgtttttat tcatagtaaa
     8281 taggattgta tttacagaaa tagttgaaaa aaagcagaat tacaacgaag atgataattc
     8341 tgttgcaata ttcaaatagt tcgctaaaaa agattacaat ataagcctat tctttgtctt
     8401 tatcttttcc ggtaatggta atgttcatca gcttgctcaa accatttgca ataattttat
     8461 caatactgtc gccaccggtt tccttaccca ttttttcggt tttatctact ccatgtccat
     8521 agatttccga aagaatattt tcgtatttgg cataaatgac attccttttc agcttcaagg
     8581 ttggagacag ttctccgctg gcaggcgacc actcttcgtg aaccaagcgg aagcgcttaa
     8641 ttttttcgtg ctcacccaag gttttattaa ccatatcgat ttcatgctga attttttgga
     8701 ttacctgagg ttgccggatc agctccgggt tatcacgata atgtatttta tgtttcgttg
     8761 cccaaaagtg cagatagttg aagtttggtg aaatgagtgc gctaacaaat ttttcgtttt
     8821 cgcccacaat cataatttgt tctatcaggc tcgaagcttt gaacatattt tcgataacct
     8881 gtggtgctac atattttcca cttgatgttt taaaaatttc ttttttacgg tcggttattt
     8941 taagaaatct gccttcaatc catgtgccga tatcacctgt atggaaccac ccttcttcat
     9001 cgaaaacttt ggctgtttct tcaggatttt tataatatcc tttcatcaca ttaacccctt
     9061 tggcaagaat ttcgccgtcg tctgcaattt ttatagtaac accttcgagt accggaccca
     9121 cggtgccaat gcgtttattt ttaaaatcag gccagtttac tgccagcacg ggagaagttt
     9181 cgcttaaccc atacccttcg caaataggaa cacctgcggc atggaataat ttgttcaatc
     9241 tttcctgcaa cgatgaaccg cccgaaacaa cagcaattac gtttcctcca aaagcagcct
     9301 gccacttact aaacactagt tttcgggcta actttaattt tgcattgtaa aaagccgaat
     9361 tatttccatc gcttttatat ttcacaccca aacgaattgc ccagaaaaat atatgccttt
     9421 taattcccct taggtctttt cctatcgcaa taattttatc gtagaacttt tcaagcaaac
     9481 gaggtactga gttaaagata tgcggtttaa cttctttcag attatcaaca atcgtaccca
     9541 tgctttcggc atagtatatg cgaataccct taatctgaaa agagtaattc accatacgct
     9601 catacacatg gcaaaggggc agaaagctaa gcgctttgtg tccgaattga acaggaagaa
     9661 tatcgtaaac ggctaaggca ttcgacagaa tattccggtg cgtaagcatt acccctttgg
     9721 gatttcccgt tgttcctgat gtataaataa tggtatgcaa atcgtcaggt gatatttcgg
     9781 cagatcgttg ttcaagtatt gatttaattc tttcctcctc atccttgcct ttttgaataa
     9841 aataaaccca gttttcggca tcttcaacat cgttgaaagt ataaacagcc tcaatagact
     9901 caatgttgct caacaccggc tgaagtttgc ggtagatgtt tttatcggat ataaacaata
     9961 attttgaatc ggaatgtcgc agtataaatt ctgtttcctt ggcgcttagg gttggataaa
    10021 ttggtatatg cacaagacct gccatcgaaa tgcccatatc agcaatgttc caatgcggca
    10081 tattctgcga gatggttgca attttatcgc ccttttggta accaccggca ataagaccac
    10141 aagcaaataa ataggaataa tgatgataat cgtaagccga aaagttaatc cagttatcct
    10201 taaacttata gcttaacgca tcgtctgctg agctaaaatc agttttataa agctccagaa
    10261 tgtcgaatat tcgggtgaga ttcatttccg tgcagcttgt ttggttttac ttatattttt
    10321 tatatttacc atcagccttt tcataagggc atgttccaca atgtgcaggg tgttttatca
    10381 ctgttgaaag ccagatgcgg taacccagcg aaaaggtaaa agtttcgtgc tgatgtccgt
    10441 ataaaacaga gcaggtaata tcgcctttat cgtggaggaa aaaagcatat tccagttcag
    10501 caaaagggca gatgcgatac tcccaagtgc cgttggtttt aaagcctgac tcatacacat
    10561 aacccggaat ggtgttccac aactgtcttc cgacaatgct tccacccaca gccacatgca
    10621 atgagttacg ccagctttgg aatagcctga atttcaatgc agcattaaag aaaaacccgg
    10681 gtttcgatgc tgcgtcgcta taaaaaccag gcataatgcg ccacgagaaa taatcagatt
    10741 taataagaga ctctatcgat accatcagca atggttccgt tacaaaccaa gcttcagaat
    10801 cgatcctgtt ttcgaacaaa tgtctgtttg gtgtgctaaa cggatgaaca ccgattattc
    10861 ccactttggg atgtatcatt gtttgagcat gtataatgcc cgaaaacgca aaaacaaatc
    10921 ccagtaaaac tattttcttc gaaatgttca ttcgatgatc ggtttttcta agcttacaaa
    10981 tataacgcaa aattcatcaa tgtattactg ccttgtttct caattcaaca aattgcttgc
    11041 cccactattc agctatttga atagtgcttt tgcgtaattc attaacccta attttcgcaa
    11101 ttctttcaat atcagttcct tattctcggg tttccaccag aaaaagtact tggtttgccc
    11161 ttttttatca tccaccgtaa aagcaactgg gatcctgttc cctgtgtatg ggtcaattcc
    11221 cgtatagtac atcactgttg ccaacgtcat tggtgttggt gtaaaatcct gtacctgttc
    11281 cagtttatat ccctgatctg cagtaattgc agcaagttct gccatatcag agatagtgca
    11341 tcccgggtga gaagatataa aatagggtat aagttgcaag cgtcgttgcc cttttgaatt
    11401 catggtatcg aaaagttgtt tcagttgaat gaaatccttg tacgaaggtt ttcgcatcag
    11461 tttgagtact ttttctgatg tgtgctccgg agcaaccttt aaacgccctg atatatattt
    11521 ttcgattaat agcttcaggt aattccgatt tgcgcgattt acctcttcat ttttggaacg
    11581 aaacagcggg atatcgtagc gaacgccact tccaatactc acatggttaa ttccgttctg
    11641 ctctgcaacc gatttataca aatcaatcaa gggctgatgc gaaatattca ggtttttaca
    11701 tatagccgga aatatgcagg aaggtcttga gcacttttcg cacaactgca ggttttcacc
    11761 attcatgttg tacatatttg ccgaaggacc gcccaaatcg gatatatgtc ccgaaaaatc
    11821 aggcattgca actatgcgct tcacttcatc aataattgag tttttcgaac ggctgctaac
    11881 aaatttaccc tgatgtgccg aaattgtaca aaaactacag cctccgaaac aacctctgtg
    11941 aattgtaaca gagtgtttta tcatatcata cgcgggaatt ggcggtttat ttcggtagcg
    12001 tggatgcgga aggcgtgtaa acggcagtcc atatatatta tcgagctctt cggcgcttat
    12061 cggcggtagg tgcggattca caacaacata aacattaccg gttttctgaa ccaacatttg
    12121 ggggttcatt ttattagatt ctgtttctac gcttacaaaa tttgcagcat actttttttt
    12181 atcagtgcta cactcttcga atgaattcag aatgagcaga tttcccttta gattctcgta
    12241 actgcctgtg caatatgcaa gctgcggtat ttccgatgta tttgttcctg cagccaatgc
    12301 tttggcaagc atcgcaacag gtttttcgcc catcccgtaa accagataat cggcttctga
    12361 ctgaattagt atcgacggca tcactttgtt ctgccaataa tcgtaatgag caaagcgcct
    12421 catcgatgcc tcaattcctc cgagcacaat cggcgtatcg ggatacaatt gcttcagaat
    12481 cttagaatat accaccgtcg cgtaatcggg ccttgctcct gctttatctc ctgcggtata
    12541 tgcatcattc gatctcagcc ttttattggc agtataatga ttcaccatac tgtccatatt
    12601 cccggcactg acaccaaaaa ataagcgtgg tttcccaagt tttctgaaat ctctcaaatc
    12661 gtcctgccaa ttaggctggg gaattattgc aactctataa ctttcgtgtt gaagaaccct
    12721 ggcaattacg gctattccaa aagcgggatg atcgatatag gcatcaccgg taaacagtat
    12781 tacatcgata taatcccagc caagtgactc aatttctttt tttgatgttg ggagaaaatt
    12841 ctgaaccaat atatcccggc tcatcattac cagtacaatc cagaataggg gttggcctcg
    12901 taattaccaa agcgggtggg gtcaccaaaa atacccccgt tagagtaata cagatctgtt
    12961 cctgggtatc cgctattaat ctggaaatcg gcgttgaacc acgtttcctc agaaatttta
    13021 aaagtcattc ccacatgacc cgctttataa ccatattgcg atgcccccgg agttacttcg
    13081 gcaaatccac cggcacgtac ccatagtctg tcgcttacct gatattcgcc acgggcatac
    13141 aaatagaaac gtgcttgttt cgaagcttcc gattccaggc cagagtcatc gccaaaaaca
    13201 gaattcccgt acatgctgta tgtaaacatt gttcctgccg taagcgagaa cttcggcgtg
    13261 aatgaatatc tgatttcggg agatatgtaa tttgtgaata aggtgtttcc atagaatgag
    13321 ccaagacttg agcccatatt cagacccata ttcaatcgtt taccgcttga aaaaggcggt
    13381 gtaccggcat catattgcag taggttcact gatgaatcag gcgaaacata atagttttct
    13441 tcctgagcat taacataccc tgctattaaa ataagaagcc cgaaacttaa aactaacgac
    13501 ttcataaatt gctttgtttt cgaaacgtaa aaatacggtt ttaattgttt ctaacaaagt
    13561 tttttattca caaggtaaat caagtttgtg caagtcacat tctgagggaa acaatcgtta
    13621 aaaacttaaa ataaaaatgg gtaagctact tatatcgcac cgctacaacc gcctaccctt
    13681 gctaccttcc ggtcctgggg gggttcagca ggagctggtc gtataagact tacccgatgc
    13741 aaaggtaaca cattttgcat tgttttcaca caacaattca atatttctta aaaaaacata
    13801 tctttgctta aaatgaatac actatgaaaa acaatctaaa agtaatactc cggtatggat
    13861 taatactcgg tttggtaaat attgcattct cggtgcttgt gtacgttttc gatgtagttc
    13921 tcatgggaat gtttgcagga ttttttattt ccctgatcag cttgcttatc attataattg
    13981 cattggtttg gagtggaaag cattaccgta acaattataa tggtggatat atcaaataca
    14041 cgcaggcatt gttgttagca gtgatgattg ttgccgttgc tgcactggtt gttttttgct
    14101 atgatgttgt tttccgaacc gcaattgaac ctggctacga cgaaagggtt aaaactgaaa
    14161 ttactcagaa aaccgttcag tacatggaag aaaaaggcgt tcccgatggt gaaatcgaca
    14221 aagtaattga caaaatgaat gagcaaaaac ccaagccgct agcaattaag ttggtttgga
    14281 gtttctttgg tcctctgatt tttggagtga ttatcgctct catcacctcg atttttgtga
    14341 agaaaaacaa gcctttattc gacggtgctg aggaaacacc cgattccacg ggatcgcagg
    14401 aggtaatgta atgaacatat ccgtaattat tcctttatac aacgagtcgg aatcattgcc
    14461 tgagctgatg caatggatag caaaggtaat gtcgcagcat gattacagct atgaggtgat
    14521 aatggtcgac gacggaagca acgataattc atgggaggtt atcgaaaatc tttcattaca
    14581 atatccggcg ctaaaggcaa taaaattcag acgcaactac ggcaaatcgc cggcattgca
    14641 aaccggtttt caggctgcac atggggatgt tgttattact atggatgccg atttgcagga
    14701 tagccctgat gaaatccccg aactgtaccg gatgattgtt gaaggtaagt tcgatctggt
    14761 ttcgggatgg aaacagaaaa gacatgaccc catatcgaaa cgctggccca gcaaattctt
    14821 caatcaaact gtccgcgtaa ctaccggtat aaaactgcat gacttcaact gcggactgaa
    14881 agcgtatcgc cagtcggtgg taaaaagtat tgaggttttc ggtgaaatgc atcggtatat
    14941 tcccgtgctg gcaaaaagag caggctttaa aaaaataggc gaaaaagtag tgcagcacca
    15001 agcccgtaaa ttcggatatt caaaattcgg actcgaaaga tatatgaatg gatatctcga
    15061 tttgctcacg gtgttgttca ttacccgctt tggcaagaaa cccatgcata ttttcggagt
    15121 gctgggtagt ctggtttttt tacttgggtt cgttgccgca gcatatctgg ggattcataa
    15181 actgattgcg gtgcacaacg gaatccacat cgaaagagtt acctccagtc cgtatttcta
    15241 tttatcgtta acatgcattg ttctcggcag catgcttttc atgaccggtt ttttagggga
    15301 gcttatttcg agaagtgcca gcgacaggaa tcattatctg attgaaaaag aaatttaaac
    15361 ccacaccata tatggcaaaa gaaagagaaa tcgatctggt ggaactatat attaaaatag
    15421 tttcgttcct taaaagaagg tacctgttgt tgataataat tacagtaatc ggtgttggac
    15481 tgggattagc acgccatttc actgcaccac cacggtatcg agtgattatg atcgcctcat
    15541 ccgaaattct cgataagcaa tatgtatatg cccttgccag cccgatgagt atgtacgtgg
    15601 aacaacgaaa atacgttgat ttaagcaact atctcggatg cgacgtgaaa gcactatccg
    15661 cagttaaagc ccttgaactt gacagcacat tgctgaacag tgttaagctc gaaatgatct
    15721 tttccgattc gtcctcaatc aaagcgttcc gcgatgcagt tattaatttc tacaacaaac
    15781 agaaatttat tgttgcctac tacgaaaacg aaaagaaaaa cctcaacgac tttgttaaaa
    15841 caatcgattc cgaaattgct gaactgaatg aatttcaggc taaaatgctc aatcaggaga
    15901 acagtaagaa taatatcgaa atctacacat caatagccgg ttcccatgaa gaaatgatta
    15961 ctctttacga aaaaaggtac gaattgcgtg gtaaaatgat gcagacacag cccattagtt
    16021 ttattaatga cggattggtg atggaaaaga acaattccat tgctctcggg ctgcttattt
    16081 ggggaggtgt tttctttata ctggcactta ttacaggcat ttatcttgat ttaaaactcg
    16141 ctgcacgaaa ccggagaaac tcaaagcttg tgtaatattt cggcgaattc ttttgccaac
    16201 acctgataga tgtgcttttt caacacgaat tgcttaccct tttcacccat ttttattctc
    16261 tcttcttcgg gcatattggc aatcgcttcg atagcttcag caatccgggt tttattctcc
    16321 ggctccaccg taattccgca acccgactcc ataacagggt cgttgcctgc ttccagcgcc
    16381 tgaacaatgg gttttgccga aaacatataa tcgaaaagct tattggcact tacaccatac
    16441 gcatagagcg gacttttatt ccaggtaata attagcatat ccattttacg gagcagggta
    16501 acaacagcgc gcttcggtac cgcatcaagg aaatgcacat attttctgtc ggaatcgctc
    16561 atctgctgaa tgattttttc tttttcgggt ccatttcccg ttataatcaa atgtatccta
    16621 tcattctgta tcagtctgcg ggaatcaatc agcatccgga tattattggc gaccccgata
    16681 ctgccggcat agcctaccag taatgcgttt tcttttttca accgatgaat caactcattg
    16741 tgtgcctgtg gtaattcaat ttcttgttca gcatcgtcgt acaggtaaat accattgggt
    16801 acataatacc atttttcttt tttcaatcca tgttcctgca tatgttccag agtgcagggc
    16861 aagagcgaaa ccacagcatc ggagttacga taggcatagt tttcggcttg ttgcattatg
    16921 taaataaaag gatgcaattt actatacccg cccaacagac gcggtgataa aggccacaaa
    16981 tcgtgcacct cgaaaataag ttttgcattg ttttttcggg caagtttttt cagtggataa
    17041 atttcgagtg gatacgttga agaagctatg attacatcgg gattgaagtc ggtaataact
    17101 ttagctgccc ttgtgtacag atgccggaca aattcaaaga tattcagtat ccgctttagt
    17161 ccatttccac aatagggttt ggttttaaaa agtgcataac taacaccttc gatttcgtgc
    17221 aatccgttgt tggttaccac cggttgtttc gacctcaaat gactgtaaga cgaggcaaca
    17281 atcattactt catatcccat tttctgccac aaaagtgcca tgtagtaggg tcggaattcc
    17341 atacccattt ccggtgaacc ggcataatgg tttactaaaa ctatcttttt aacctgtgaa
    17401 gtattgttta cagaactgta cattgtttgt taacttttta tctaaaactt catcccatgc
    17461 caacgttccc caatggataa taccctcttt tcggtaatta ttccacaatc gtgcccacac
    17521 ttgttattac cgttccattt tcgatataat atactgtaaa tgtaccggca ggtagtccgt
    17581 tcacagcaac aagttgttct gcaaatgtaa gaattgtttc gtaaacaggt tgccctgtgg
    17641 cttttgtaat cattatttta ccggaaatac tgctgtttgt taccagtact gtaaaactgc
    17701 cctgattagg attcggattg attgcaatac ttacctgagc ggtttcttcg ctggtactat
    17761 tcgcattttg aacctcatca tgatctgggc tatcaaaaat tgttaccgga taattaatat
    17821 tacattctgc aatggattgt tcatattctt cgggcaatat agtgccttct ctcaatgctt
    17881 tttctgcact tacaaaacac aaccaatttt cgacaaaagc ttttgcattt accgtgtcag
    17941 gtatttcgtt tataagaaat tccaattgcg ttatcgacct gttgtagtcc tttctcagat
    18001 agttcaacat agcaatgtca agcttgtatt cgatacttaa ataatcaggt atatcagggt
    18061 ttatcaataa atcttcatgt agaagaacca tccggtcgac agcttggtta aaaccattat
    18121 ggccggaatt gtagtcaata aattcataat aattggtcaa ggtattatga acatcgccat
    18181 aggctttgtt tttaagtaaa aatacggcat cggaggtatt gttgtcgcat tgaaaaatca
    18241 ggttggcata ttcatcaatc aggttgttga atgcgttagc agatgaaaca ccatcggatt
    18301 gatttgccaa ataagtaacc gcctgatcta atggtaaact tattcctccc gatagtgaaa
    18361 ttaatggtaa actgtttaat aaattggcgg tttcttcatt cgattgaaag caggagcctc
    18421 cttcttgcag cgaatccgga tcgatatgat agcaaaccgt ataatcagga atactatcaa
    18481 caagttgaac tagtactaat gggttactac actggaattt ccagagttta tagtttgtgc
    18541 cataatcagg tccttcggtg tcatctgcct cccagcggtt attgtttgca atattttgtg
    18601 gagaggcact gcaattacct ggctctaccg aaaacccgta aacagcatac gcatcggtgg
    18661 ctgcctgtag ctggttgtat ccctcgttaa gaaataaacg accataattc aggtaaattg
    18721 agcggacatt actatgtata tcgttttcag cattgttttc ggcataaaca taggttccgt
    18781 tttgggtgta cacgccatac cggtttgcgt agatgttgtt acacgatagc ctcaggtcga
    18841 atgcacccga agtgattata ccatcgtaaa tattgctata aatatcgcat gcctctacgt
    18901 cgagatctgc tgcgctgctg ccataaaacg tgaagccatt atggccgttg tggtgaaatt
    18961 cgcaattcga aaattcggaa ggtaaactca ttccgtaaca ataagcgccg tttgaattac
    19021 aatgattgaa attacagaag cttgcatgaa ttcctcgccc ataaatgtgt atcccattcg
    19081 tgttgtatcg aaattcggaa tttgacactt ccaatggact gccataaacc gataaatcgc
    19141 ccgtaattcc ataacttgaa tattcaaata tgcaattgtt gaccacagtg ttttcttgcc
    19201 cgtaaaagca aaagcccctg tgtttgccgt tgataccggt tgcagaggtc atcagtacat
    19261 tatcgaaagt aattggataa tcggcagttg ctaacatttg atcgcctgcg ctcatgacaa
    19321 ttttacaatc ctcaaatgtt agtgattcta atgcaggaaa acgcactgca tcttgttgta
    19381 cttctacaac tttatcgctt ttccctgaac cctgaaataa catagaagct cctgttccgc
    19441 aggtgatgtt ctccgtgtac atatcgccgg ggttgctgaa tttgatgtag ccggcaccgg
    19501 tgaaggtgaa ctgggcgttg gcagcaatgg agaggttgcc ggagatatca aggcacgtat
    19561 tattatctac caaatcaatt gtcgccccat tataatatac taatactgca ggatttgtag
    19621 tatgggattc aatttgaatt gatccatttc ctgaaacggt cacacttcca ccgtttaaaa
    19681 tttctaaagt acctccgggg ttgacaacaa ttgatttgtt ttctaataat tgaatatttg
    19741 agttatttat tactaatgtc cctccatcag caatactaat atctttttga atatataaat
    19801 ctctatttac cactatttct tgctttatta ctgaaggcag ataatcgtaa tcaacaggaa
    19861 tgcgattaaa tccctgtgta attgtatgcg gcacgaagct tggcagacaa tttacaacta
    19921 caggaatccc atcttttgta tcatagtcaa caaccgatac atttagcact tcaccggcac
    19981 caggattttg cactaaatgt aagtctaaaa agaatttagc tggaataata ttacttagtt
    20041 tcctcgtaat atccaattca atttcaattg gatattcatt gatgccttgc attggtatat
    20101 gaccagatgg catccaataa gtctcatatt gttctatgaa ttgaggagtt gtgctggcag
    20161 agtcagtgct gacaccggtt ctcggattaa catatgtgcg ttgtgaataa tccatttcaa
    20221 ttcgcaaagt aactttggga ttataaacag tattttcagc aattacaatc ccagaaagat
    20281 aaatcgaata aaacaaacga taagggatat aaacaaaacc atcatttgcc catcctgcac
    20341 cccaagaatt tgccacttta aaagctccta tttcccaatt ggatatgatc ccatcatttg
    20401 tgtataaccc gtctccgtta aaatcccaca ttacgttatc gttgtagccc acgatggtca
    20461 tggcgtgccc aaacccgctt gtaggccatt gtgttactac ttttttacca gcttcagcac
    20521 tcggataaac caaatcgttt attacagggt tttgaaaatt ctcaccaaac gacaacagtc
    20581 ctcccccttt tacaagatca ccaataccga ggtcgtgaag ataatgttta atatcgtcca
    20641 aatcatctga ggtcattaat tctattgaaa atacagattc tataactcta ttgttcaacg
    20701 ctaatctata atcagagtac cccgacatcc acacggttct tctttttgta aaattcgcgt
    20761 cgtcgtcagc aaacgttcga tacaaacccc cataagatgc aatatttgga ataccagatt
    20821 ttttaatgac attccaacca gttgatatac cagagccatt atttgttgca ttattgcaat
    20881 aattccaagt gtaatgagtt ggataggttg tttcattact atttgaagaa atgtttcgtt
    20941 cccgatttac catataagta aaaacatatc caattgttga agcctgtaca caactgccgc
    21001 cgtcttgcga aaaaattgaa cggaaataag gttttagcga attatcaaca accgggggca
    21061 attgaagatt ttttgaatag aatgaaatat acggccctgg caacgaatca agataagctt
    21121 gctcccctgg gctaggttca taacctccta aatattgaga tttcacctga accgttgcaa
    21181 gtaaaagtat tatacatgca aaaaatgttt gaaatagtcg tttcatgatt ttatagttca
    21241 gtttttaaca gaacacttga tttttctcac ttaattttaa caattctctg ctcaaacctt
    21301 ttgttttcag cttcaatcaa cagcaggtaa accccatcat ctaacccgct tacatcaatt
    21361 cggctattgc tgatgtagtt gttttcactg catataagcc gtccgtacat atcaactatt
    21421 tttattgtaa ccgatccggt gcttcgaggt tttgtttgtg attttatttg taaataatct
    21481 gcaacaggat ttggaaatac cataaacacc tggttgctaa tgagttgtgt ttcgattttt
    21541 gtataaacgg aattttggct gcggtgcaga tgattgccat aggcatataa atacccgttg
    21601 ggcgcatagc ataacccgta aacattctca ttatttaaac ctatgttaat catttcccat
    21661 gtttcgccgt agtcggttga gacaaaaacg ccaccctgag ttccaaattc attagagcat
    21721 ccaatataaa tatggtcgtc gggagtaatc acaattgatt ctacaaaaac atcgtttttt
    21781 agcccagtcc atgttaaacc ctgatcctgt gaacgataaa taccaattcc gtaatttcct
    21841 gtactaccgg cgtacaaatt accattcgaa tcgcaagcta aggatttaat gtaatatcca
    21901 cttagttctg catattccca tgtttctccc tgatcaaaag agcgatatac accaccggca
    21961 tcagtcataa atgatgtgat ccctgcatac aaaacattat tgtggtcaga acaaatacta
    22021 tttacaactt gatcattatc aacatctaat gtttgctccc agtcttctaa atcattggtc
    22081 ttgaatattc ctccccaaaa acctattaat aaataatcca tattagaaaa tatggataaa
    22141 acattaccta ccccagaaaa tatgggtgtc cacaattgtt cattgatatc gaaattgtag
    22201 attgaattaa aaccgccagt tcctgcaaaa atacgctcat taagatctat atgtaaacta
    22261 tatacagaaa gaccgtcaag ctcatgcaaa tcccaattgt agttttcagt aagactacca
    22321 taaacgccac tatccattcc catacccaaa tatacagtgt ttgtattagt tacagccatt
    22381 gcataaaccg gcttatcaat cggtcgttct atctctgtcc aaaaattttg agcgtttatt
    22441 tgcccgataa ctccgataaa taaaccaatc attgctatta ttctcatgac aagactaata
    22501 aagttactaa aattactcca caatcacacc cacacttgtt attaccgttc cattttcgat
    22561 ataatacact gtaaatgtac cggcaggtag tccgtttaca gtaacaagtt gttctgcaaa
    22621 tgtaagaatt gtttcgtaaa caggttgccc tgtggcattt gtaatcatta tttttccgga
    22681 aatactgcta tttgttacca gcactgtaaa actgccctga ttcgggttcg gattgattgc
    22741 aatacttatc tgagcggttt cttcgctggt actattcgca ttttgaacct catcatgatc
    22801 tgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgtagc cggcaccggt
    22921 gaaggtgaac tgggcgttgg cagcaatgga gaggttgcct tttatttcaa tataagattc
    22981 attatctaca agattaatta ttgccccttg attaaaaata agaaccgaac tataatttgt
    23041 atctttgcca acatatatac agccatttcc gttaatattg acaattgatt catttcttag
    23101 ctcaacaatt ccccccggtt caacaattat tactgaccat gttttatcgc agaaacttgt
    23161 taaataccca tctgattcta aaactaagga agctccattt ttaaccacaa tatttgaatt
    23221 ttcattaaag tatactctag tattaatggt aagatgagag ttagcttgta ataataatgt
    23281 accattaatt gatttaaacg aattccacac tattgtttcg ccggagtata ttgtttcact
    23341 attgccataa gctttcttca tggcttcaaa tgcatctgct ctcccactac ctaataattc
    23401 attataagca atattttctg gaatatcatc aatatcaaca gctgtactta aaagaatatc
    23461 gactgcttca tccctggaaa ggcatggatt tacagataat ataagcgcac aaataccagc
    23521 tacttgtggt gatgcaaacg aagttcccca agtaccttga tatgtattgc cgatagaagt
    23581 cgaaggaaca ttgtaacctg gagcacagat atcaactgcc gtattgtggt gatgagctgt
    23641 tccaggatct ccgatgacag cctcatgcac atctttccaa tttgtttgcc cataaattga
    23701 atctgtataa ccaattggat tcatatggcc aacagacgtt actgagatgc agttatcata
    23761 tgaggctggg aatacttctg catccaagct accacaatga gctattgaat ttccagcccc
    23821 acaaactgta atagttccat tatctttaat ttcttcaaaa actagccaat caatatttga
    23881 ataagcacat ctatctatcc aacttgcatt tattactcta gctccactct gcgataataa
    23941 ta
//
LOCUS       DAOOQP010000034        23332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2668_length_23332_cov_5.909610, whole genome shotgun sequence.
ACCESSION   DAOOQP010000034 DAOOQP010000000
VERSION     DAOOQP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_23332_cov_5.909610"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLK75_05405"
     CDS             complement(<1..758)
                     /locus_tag="PLK75_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38718.1"
                     /translation="MLAFLGFQNLYAQTETIRAGSFIINMGNTNTASANDDLKPYGMI
                     LDLMLNYHVPVKWVINPSKLKDGTDFTHNGVAYKGGTFIIPREYRTDPIDARIAYWQT
                     QGVVGNFSVSPFQVNVSFTFTAPPHWVLNSENTGIITSYLTAAGITAAAYPTVISYKL
                     AAALDCCDDIFAMPHADPLWSSHNRLISWNDECKGAIYASCHAVSVLENIVNPANRSQ
                     QANFLVQKDPAYTGTSGDYANSNTICIKAFYNDT"
     gene            complement(1780..2631)
                     /locus_tag="PLK75_05410"
     CDS             complement(1780..2631)
                     /locus_tag="PLK75_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY38719.1"
                     /translation="MEKQIYEEKVQPVLVYTDFTATGNKAVEWAVYMCEKMSRRLILL
                     HVIDRNTYSLAGNTDPEIFARETISEIRGKIQISTTVKVSTHVEEGCNCTVISKVAEE
                     NDVIFSVVGVHARNDLQYLSGLTAIKMARRSRIPFLFVQEMTPTPHSLAKIAFPLNIQ
                     KQMKEKVSWGIFLAKHLHETVEIVLPENDDEVKTNLFFTAMLFEKLNVSFVQTKINGN
                     IFNVENRALNYTEKNRLLLSVMLMLKGNSVMESFFGPRELKYVCNRQKMAVFMVNPRK
                     DLYIPCI"
     gene            2680..3912
                     /locus_tag="PLK75_05415"
     CDS             2680..3912
                     /locus_tag="PLK75_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOY38720.1"
                     /translation="MAFTRNITDDQILKLTNNVTWTGVLDAEIRTFDIVMETEYGTTY
                     NSYFINADKKVLVETVKEKFFKKFLSKLQQLVNPADIDYIVLNHTEPDHSGSLVHLLK
                     IAPKAVVVGSGQAVKYLEDIVGHSFKHQIVKDGDTLDLGNMTLRFIGAPNLHWPDSMY
                     TWLQQEKILFTCDSFGAHYCDKAMFDDMTGDYNEAFDYYFNVILKPFSKFMLKAIDKI
                     SDFDIRMIAPGHGPILRSTWKEKVHKSKVLSEEYVAENTHKRKKVLVAYVSAYGYTRE
                     MAELIADGIRKHDDIEVELADIETIELGELDSKLVSADALLVGCPTLNQNILLPVYKL
                     FALINPIRDRGKMAAAFGSYGWSGEGVHIIEAVLKSLKMNVISEPKPMKFRPGERNNH
                     ELFEYGKSFGEKMKQEIG"
     gene            complement(4453..4989)
                     /locus_tag="PLK75_05420"
     CDS             complement(4453..4989)
                     /locus_tag="PLK75_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOY38721.1"
                     /translation="MALDITLLIFGFALIVLGVIGCIVPAIPGPPLSYCGILLLHFTK
                     YCHFSSTFLIVWAVVVVAVTVLDYLIPVWGTRKFGGSKWGAWGSVIGLIVGLIYSPVG
                     IFLGALGGALVGELIYQGRLNRRENSPKLSGKERFNKSLKAAFGSFIGLIFGILLKLT
                     VSIWLAVAFIKKAILALF"
     gene            complement(4989..5420)
                     /locus_tag="PLK75_05425"
     CDS             complement(4989..5420)
                     /locus_tag="PLK75_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38722.1"
                     /translation="MKNIFFLLAVGIIVSLSSCKPSQPCAESDYRDTDDMLYACGQGS
                     GSRENTAVEIAIIDAKQKMGEKIGLYTAEHYKSETLVNDEAYNNKLRTAEKTVLTELG
                     ISCQVVKKSKDKYLAYVTLSADLKVIREIMRGSSSGTKNKD"
     gene            complement(5510..6277)
                     /locus_tag="PLK75_05430"
     CDS             complement(5510..6277)
                     /locus_tag="PLK75_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38723.1"
                     /translation="MKAVNVIKRIFLTICLAFLFFFAKADITTSLDSAKNFYTKGDFY
                     EAQLIYQRLADDNYSSVELYYNLGNCYFRGEQFAPAIYYYEKALQIDPRHEKALANLK
                     IANSRIPDKINRMEPVFYIIWYNALQQMLSSNGWAYAGLGSLMITVVFAIVYLLSGKR
                     SIKILGFSMAVIFLITTAAAIFFSIKQKQAAENPKTAIVFEDVMIKSSPSNEGNNLFE
                     IHEGLKVSITDTLNNWSEIKLPDGKHGWATSASLKRL"
     gene            complement(6281..8125)
                     /locus_tag="PLK75_05435"
     CDS             complement(6281..8125)
                     /locus_tag="PLK75_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOY38724.1"
                     /translation="MKKLLFFAIGIWISIVACADGIQFSAWAPQTVEVGEQFYLKYEL
                     NQRLTPQLPDLKGFTLMGGPSTGTSTSVQIINGNMSQSNTYSYSYILRANSEGTFTIP
                     GGKVVFGGQTYTSNAVTVKVVKAGSKPKQNTNRAQSFWGNDPFYNQQPAQAQEIKSDD
                     VFIRTEVDKTSVYQGDKITATIKIYTKVDLVGFEDFRLPGFDGFWAQEVKTPEQIQFQ
                     QVQLNGTNYMVGVLKKDILYPQRSGTLSIGSVGADIVVRQRVNGGQSIYDNFFGYYQN
                     KQLTINSPEVKITVKPLPGNAPGYFTGAVGSFTVAGSISADSVKVNDALTMKITISGT
                     GNLKLIDPPDLNFPKEFEVFDPVVQLNLNSGDGEIAGSKVFEYTLIPRYPGDYNTNEI
                     RFAYFDPVAGIYKTLKTNSYNIHVYKMPGDSTGQFSSDYSRSEINYIGSEDIRFIETE
                     NFTPEKNRKFFIGSPLFIVFASIPLIAFIVVVMLLRKKIKENANIEYTKNRKANKTTM
                     KRLKKANELLKNNLTSEFYKEVMVALWGYLSDKLNLPLAQLSKENVSLKLADRGLEPE
                     LTDTFLKIIEDCELAHFAPVEGSEQPLQIFDRAVDIINLFEQKLKIQK"
     gene            8298..11138
                     /locus_tag="PLK75_05440"
     CDS             8298..11138
                     /locus_tag="PLK75_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY38725.1"
                     /translation="MQNNNFHTSLIRLVALIVSGKSLKDPIVTGHAFDSFLKFYVPDR
                     LHTDYRDRFNSFVAEYASEGKLKKISSNSVRIIRICSDINTRYNKRTKMLFLMHLTQI
                     LYPYAQSDAEVNDYIKLASASMNIDDHILSVMLEFISGSMTEISNDICKEVLPGLHCL
                     YLAESEIILIFTSQSDYYLNDRKFDKGNVTEFAPGMHIARQGEDPVYFSDIKRVFDIA
                     DSQEFSLHVNELSYSVQGKIVLQPVSFSVSKGEFVAILGKSGTGKSTLIRMLAGELKA
                     STGHFFIKKEKEPAFPAIGIVHQDEFFIPEFTVYELLLERMKLLQASGKADRKQGTSS
                     VIEVMEQTGLSTIASQVAGTPEKTQLSGGQRKRLAIALCLLQHPEIMLVDEPTSGLSS
                     ADAREIMGLLRNIASCGKTVICPIHQPSYEYLHYTDQSLILDEGGFPVYFGKTDAAAV
                     YLRLHAGITDSRATACSGCNRFDPDDLFSIISENTIDEFGKETNVRKIAPETWHEIFR
                     KSVVSNYEKPLSQLPLKRKTHKINYFCLFAHYLIRDVKRRLKNPIPLVLLLLITAALG
                     TLTGVICHSGEVVYFYGDNPNIPAGFLMMIITALFTGMIMAGNEIRGDVNFRKNEMRF
                     SGKQGLWLLTKLLRMLFLSLALSVILVTITFLLFAAPLNYALHIVLFMLIFFNGAAMG
                     LILSALLPRLSQVYFCVPLLVVPQLILSGVVIDFNNYPSFLKNNKYVPAIAEVAVSRW
                     AFEAIAVNAFVNNDYEKLLYRAHFQQHEAAYYTNYFLPQLEEIAAHDKDKAAKLLASE
                     WFANANFPKLKADVNTQISFLKQYYSHRMNEIYTEQQTMFEQLNKVYDLKELRRKNNN
                     LFLEKMLTAPQQGHSLVVTESMILRKYSPVFDLPENAGGRAHFYAPAKRLGTKYMDTF
                     IFNSLVISCINIILGLLLVVFGILHSHRLKAL"
     gene            11158..13290
                     /locus_tag="PLK75_05445"
     CDS             11158..13290
                     /locus_tag="PLK75_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOY38726.1"
                     /translation="MDNSIITIVSQQTGMSFRQVAAVVHLLDEGATIPFIARYRKEVT
                     GNLNEEQIALIKTEYDKKCEIADRKSTILATIEKQGQLNPELKASIESCWDSNHLEDL
                     YLPYKPKKRTRAMIAREKGLEPLAVYIMKQVQGDPATEAAKYLCENVKDIEEALQGAM
                     DIIAEQVNENKPARDRIRVLFKNEAVIRSQLIKAKKEEAAKYEDYFDYSEKLSKCPSH
                     RLLAIRRAEREGLLHVDISPDETKAFSLLGSLFVKNNTPSANLVIMAMKDSYKRLLKP
                     SIENEFAAESKQLADIQAIDVFRNNLRQLLLSPPLGQKRVLAIDPGYRTGCKVVCLDE
                     QGNLLHNETIYPHKPQEERALSSKRIISMVNAYKIEVIAVGNGTAGRETEQFLKRLSF
                     DRDLKVYVVSEDGASVYSASSVAREEFPEYDVTVRGAVSIGRRLMDPLAELVKIDPKS
                     IGVGQYQHDVDQKLLKESLDRVVESCVNSVGVNLNTASKHLLTYISGLGPTLAGNIVE
                     YRKKNGAFLTRDQLKEVARLGPKAFEQAAGFLRIRNAANPLDNTAVHPESYPIVEKMA
                     YDLHCSIGELIADKTLHTKIVIGNYVTDNIGIPTLTDIMNELEKPGIDPRKTVKIMEF
                     ASHIKTIDDLEEGMELPGIVTNITNFGAFVDVGIKQNGLIHVSNMANRFIKDPNEVVS
                     LHQHVQVTIIGIDKTRGRIQLSLKKSDS"
     gene            complement(13329..16010)
                     /locus_tag="PLK75_05450"
     CDS             complement(13329..16010)
                     /locus_tag="PLK75_05450"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOY38727.1"
                     /translation="MKYVFNGLTQEEVLRSRKENGSNEIAKPEGEGLWLKMLGNFKDP
                     IIIILLAALVVIVVLSFFGLTEWFEAVGIAIAVLLSVFVSTFSEYKNENSFQKLQEEA
                     SLIYNNVFRDGRIQSIPINEVVKGDYVLLQSGDKVPADGRLVNGELHVNQASLTGEAV
                     AILKTPAEADYEPEASDLEDRHFVFRGSVVEEGEAVILVEAVGAASFYGQLTVELSLS
                     DDRLSPLQMKLKNLAKLISKFGYIGAGLIFVAFVFNKIFILHNFDMGLITAYFEHWDV
                     VVNDIIHAMVLAIIIIVAAVPEGLPMMIAIVLSLNMRKMLSEKVLVRKLLGIETAGSI
                     NILFSDKTGTITKGRLDPIFMIDGKGETFNNYSDIPTALRNMVRISLVDNSYCVLDGY
                     GEPVGGNASEHALIRFVSREDRLNEHPSTQALANIRFNSAKKFSATLVKGDAALKDIF
                     LSDKVTFVKGAVEILMEKCTKIFNAEGHLCDIGSKRNLIEMSDEMANQGIRLIALACS
                     DNPIEEEGDLPEGMALLGIIGIKDEVRPESRDAIIQAMKAGIQVVMITGDRKGTAAAI
                     AKETGLLASDEDVVLTSQDLRQLTDEQVEEILPHLRVVARALPTDKSRLVRIAQNLGK
                     VVGMTGDGVNDSAALKQADVGFAMGSGSEVAKESGDIVILDDNFSSITNAIRYGRTIF
                     KSIRKFIVFQLTVNIAAVLTSFLGPLLGIDFPLTIIQILWINIIMDTLAALALGGEPA
                     LQRFMNEKPIRRDANIVSKAMSSAMFSGGIYITAFAVFFLLFPPFKDLFIRNGAPDTM
                     VFMTAFFNLFIFLIMFNSFNARTEKMNLMENIFKNKGFLPIIGFIFVMQIVFTYIGGD
                     FLRTHSLTIQEWLIILLLSVSIIPFDLARKFIYGLTK"
     gene            complement(16178..17329)
                     /locus_tag="PLK75_05455"
     CDS             complement(16178..17329)
                     /locus_tag="PLK75_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOY38728.1"
                     /translation="MARISILLVILMSGHFMMAQNLNVAIFYNDRPNKVIFKSISGCY
                     ALAENDSTVVALTPGQDVFISLFSGNIMVQANDSSHIFYGNISIKTETQGCFSVTLVP
                     AVGSARIYDGNLYVRKNNQNLLLVNNVPLENYVAAVTEAEGGYKKPFEFYKAQAVISR
                     TFALKNIGKHQAEGFELCDGTHCQAYKSKSESPDILRAVQATSGLVIVDENYALIMAA
                     YHSNSGGITMNSEDVWISSLSYLKPVEDKYYVYGRNAVWQQTITADQWKSFLKTKGVD
                     ASIPVDSSIISSGTRIARVTIGGVSIPMTEIRHYFRFPSAFFSMSMNDAAITVSGRGY
                     GHGVGLSQESAVRMAEEGFAYNEILNFFYKNIYIVNMQTLSVFKVLQNH"
     gene            complement(17316..18440)
                     /gene="lpxB"
                     /locus_tag="PLK75_05460"
     CDS             complement(17316..18440)
                     /gene="lpxB"
                     /locus_tag="PLK75_05460"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOY38729.1"
                     /translation="MKYFIIAGEASGDLHASNMMKGIRQYDAEAQFTGWGGDLMQAGG
                     LHLLKHIRELAFMGFTEVVANLRTILRNFDLVKLQIIEYQPDALLLVDYPGFNMRMAK
                     WAKMQGIKVYYYISPNVWAWKSNRVFHFKKYCEKLFVILPFEQEFYSRYNIDVVYEGH
                     PLIDAVESHRKITFNEFCAENSLDGRPVIALLAGSRKQEISRMLPIMLSVVDDFPQYQ
                     FVVAGSPGIDKDYYSQYLRQAGAVLVMNKTYDVLSHAEIGLIKSGTATLEAALLDVPQ
                     AVCYKAGLISVLIAAMVKKVKYISLVNLIMNKACVTELLQFRLNRNNLKKEIDSLLPD
                     TARRKQMLNDYADLRNRLGDPGSTVRVGAQIVKLMSDGSH"
     gene            complement(18441..18740)
                     /locus_tag="PLK75_05465"
     CDS             complement(18441..18740)
                     /locus_tag="PLK75_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38730.1"
                     /translation="MISDKLQKLNKPVIGLGLGLVFPLITFLIMFAVNVIPSFSAYIQ
                     HLKNTHALLPVMSLCVLPNLILFFIFKKLNYWYAIKGVVISVFLYTVAVVVLKFA"
     gene            complement(18746..19513)
                     /gene="surE"
                     /locus_tag="PLK75_05470"
     CDS             complement(18746..19513)
                     /gene="surE"
                     /locus_tag="PLK75_05470"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOY38731.1"
                     /translation="MQKKLILVSNDDGINAKGLKHLIEMVRPYGELLVVAPENHQSGM
                     SHSISILKPLRANLVHKEEGMMFYSVPGTPVDCVKMALNRLAPRKPDLMVSGINHGSN
                     SAVSVIYSGTIGAAIEASLYDIPSIGFSVLNHAPDANFSIVQKNGGKVIDAVLANGLP
                     KNVAFNVNFPNIDPDDFRGFKICRQAKGVWREEFVEHTDPYGMKYYWLSGNFENFEHC
                     AEDTDEWALKNNYASVVPVHADLTSHKTIDIIRNWKL"
     gene            complement(19617..21245)
                     /gene="lnt"
                     /locus_tag="PLK75_05475"
     CDS             complement(19617..21245)
                     /gene="lnt"
                     /locus_tag="PLK75_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOY38732.1"
                     /translation="MKLKWYYKLMLSLISALLFCCGWSQWGLGPLVWIAFVPLLIIEE
                     HDYINRQRFRTVNSFLWSFLSFLIWNAITTYWIYYSTVFGAIAAIFFLSLFMACVFWS
                     VAIIKRKMGPRIGLLAFVSFWLSFEYLFMNSQISWPWLTLGNAFANNITLIQWYEFTG
                     HLGGSLWVLLLNVLIFLFVRDMFLLKKKPVNRKNLISLILLILVPIGYSVVRYYTYSE
                     KPDPVDIVVIQPNIDPYNEKFETPSMDQLSDMLTLADQKMDSTVDVIVGPETAIPDGM
                     WESEMDDERTIRYLKAYLQHYPKAVFIMGADTRDYYPDGVGKSETAIAMGKTGNYYDL
                     YNTALLIDTSPAIQAYHKSKLVPGVEMMPYPKLLGFLGDFAIDLGGMSGSHGVQAERT
                     PMQKSDGLKIGTAICYESAYGEFFAGFVKEGAGIMCVITNDGWWGNTAGHRQHKSFSS
                     LRAVETRRCIARSANTGISCFVNQRGDILQATEWWKKDVIRATVNANDTLTFYTRYGD
                     FLARMSLVISGILIVVFFMLGLMNLSKRSSRNPK"
     gene            complement(21247..22080)
                     /locus_tag="PLK75_05480"
     CDS             complement(21247..22080)
                     /locus_tag="PLK75_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY38733.1"
                     /translation="MLLGFIWGASFILMKKGLNVFTPVQVAAIRMSLASLVTLPFGLR
                     LLNKINRSNIVPFMVVAFIGNLIPAFLFSFAQTRVNSSLAGMLNATTPFFALIFALMF
                     FRHKAYWKQIVGIAIGLAGAFGLVMRNGFSDMTFNAYALLIVVATCMYGYNVNQVKKF
                     LSGFTGFEITSLAYLFVFPPSIAALIFSDPTETVANPHFTMSLIYLVILSIAGSVIAV
                     IFMNMLIQKAGALFASSVTYVIPVFAIFWGIADGESITGVQIFSIFTVLAGVYLVNKS
                     E"
     gene            22169..22759
                     /locus_tag="PLK75_05485"
     CDS             22169..22759
                     /locus_tag="PLK75_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOY38734.1"
                     /translation="MKIIKFIIVLHAILFFSLREANAQMPRYIGRLINIDSIAQNVGM
                     PAFLLQEVCVFPKDQYNRQKRYNKMARNFLVVYPIALQIKDEFVQIEEDMKAMNPRSQ
                     RKYMDKKDNELKQKYKKTLMKLTLSQSMMLVKLVDRETGNTSYELIKELRGSITAFFW
                     QNIALLFKNNLKMHYDPEVSDKEIEMLVKRYEAGTL"
     gene            complement(22807..>23332)
                     /locus_tag="PLK75_05490"
     CDS             complement(22807..>23332)
                     /locus_tag="PLK75_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38735.1"
                     /translation="YMFVASKRDAISYGSFMEGENTYEYTRQVWEMDIFHKISMPLRL
                     NLLLNIKDFKFSLFAGYNLNVVITARDYYRCDYIVPDYDEKNRYTVIDYNPANKNETQ
                     LSLNRLTSQISLGMSFYVTPNLSVNLISEWGIRGFYYDYVPLDWDIYIDFYYFHGGTR
                     SNNNFILSATYFLK"
BASE COUNT         7071 a         5007 c         4693 g         6561 t
ORIGIN      
        1 tgggtatcgt tatagaatgc ttttatacat atggtattac tgttggcata atcgcccgag
       61 gtaccggtat aagcagggtc cttctgcacc agaaaatttg cttgctgact tcggtttgcg
      121 ggattcacta tattttcgag tacgcttaca gcgtgacaac tcgcataaat agctcctttg
      181 cattcatcat tccatgatat caacctgtta tgcgacgacc ataatgggtc ggcatggggc
      241 atggcaaata tgtcgtcgca gcagtcgagt gctgcagcca acttatatga aattaccgtt
      301 gggtatgcag ctgcagtaat accggcagca gtaagatagc tggtaataat ccctgtgttt
      361 tcgctgttta gtacccagtg tggcggtgca gtaaatgtaa aggaaacatt tacctgaaaa
      421 ggcgataccg aaaaattgcc cacaacaccc tgggtttgcc aataagcaat gcgtgcatca
      481 atcgggtcag tacggtattc tctcggaatg ataaatgtgc cacctttata cgccactccg
      541 ttgtgggtaa agtcggtacc atccttaagt ttcgacgggt tgataaccca tttcaccgga
      601 acgtggtaat tcagcatcag gtcgagaatc attccgtaag gtttaagatc gtcgttggca
      661 gatgcagtat tggtattgcc catattgata ataaaagatc cggcacgaat ggtttcggtc
      721 tgcgcatata aattttgaaa accaaggaaa gccaacatag aaaagatggt tctactgcaa
      781 ttttagtggg taaatattat gaaatcgcac gtaatgacta aataatcaac aagttaaggc
      841 taataactct caaagcctat aatttatggc tatatgtata gtaatcaatt gtttaggtgg
      901 gatttattga aaactttcca ctaaaattgc agtagaaccg aaaagataaa gccaagtttc
      961 agcgatttat gaatcagttt atgccaaggc aaattcgttt gcaacattgg tacagtaagc
     1021 tgttcagcgc atttaggctt gccaatacta cttttcggct tagtcaaatt atcagagaac
     1081 atttttatca taattttaag aatttcagta taacaacaaa aaactaccta ttttgttgaa
     1141 acattgttta ttgcattttt actttttaat gtttattaca tcattcatac gaataaaatt
     1201 agcgtagttt tgcaaatata atcatttaat agttttatac aatacaggtt gtaagtttta
     1261 tttatgtaca tattgtgaac aatattgtat gtaattgatt atcttattat tacgtgttat
     1321 tcttattgct aaataaaatt aatttatcaa tttattaaca ttttcataat gttaaatccg
     1381 attaatacat tttgcaaaca ttatggcaat tgcaacaggg ttgtgctaag ctttcgattg
     1441 aacaataaat gattacgttt aaatggattt gagaatctgt cgttgtgttg gttgttggcg
     1501 gagtcgagaa ccggtcgtcg agcggagtcg agacaatccg ggtacaaaag ccctttgtac
     1561 ctagcccctg tcgtgctgag ggactccccc tgtcatgctg agtagcgcag cggtaatcga
     1621 agcatgaaca ggggagttga agaaatacaa acgaagcaac tccgaagtca cccttcgata
     1681 cttcgacagg ctcagtataa attcccactt cgctgcgctt cgttactcag ggtgacaaaa
     1741 ggcaatgtcg tgctcgcatg acgttagtac gtatgattgt caaatgcagg gaatgtatag
     1801 gtctttgcgc ggattaacca taaatacggc catcttctgc ctgttgcata catatttcaa
     1861 ttcgcgtgga ccaaaaaatg attccattac ggaattaccc ttaagcatta acataacaga
     1921 taacagaagt cgattttttt cggtataatt caaagcccgg ttttcgacat taaaaatatt
     1981 cccattaatt ttggtctgta cgaaactgac attcagtttt tcgaacagca tcgcagtaaa
     2041 gaaaagattc gtttttactt catcatcgtt ttcaggcaac acaatctcaa ctgtttcgtg
     2101 aagatgcttc gctaaaaata taccccacga aactttctcc ttcatttgtt tttgaatatt
     2161 cagaggaaaa gcaattttgg caagcgaatg gggtgtaggg gtcatttcct gaacaaaaag
     2221 aaaagggatt cgggaacggc gtgccatttt aatggctgtc aatccgctca ggtattgtaa
     2281 atcgttgcgg gcgtgcaccc ctaccaccga aaatatcaca tcattctcct cggcaacttt
     2341 acttataacc gtgcaattac aaccttcttc cacatgagta ctcactttca ccgtggtgct
     2401 gatctgaatt ttacctctga tttctgaaat agtttcgcgg gcaaagatct ccgggtcggt
     2461 attaccggcc agtgaataag tattcctgtc gataacgtga aggagtatca gtctgcggct
     2521 cattttttcg cacatataca ccgcccactc aacggcttta ttaccggttg cggtaaaatc
     2581 ggtgtacaca agtacgggtt gaactttttc ttcgtagatt tgtttttcca tagcgcgaaa
     2641 atagtatttt tatcgtttaa ataataacta aacaataata tggcatttac aaggaatatt
     2701 actgatgatc aaatacttaa actcacaaac aacgtaactt ggacaggagt tctcgatgca
     2761 gaaatcagaa catttgatat tgtaatggaa accgagtatg gcaccaccta caacagttat
     2821 tttataaacg ccgataaaaa agtgctggtt gaaaccgtga aagaaaagtt ctttaagaaa
     2881 tttctttcaa agcttcaaca attggtgaac cctgccgaca ttgattatat tgtgctgaat
     2941 cacacagagc ccgatcattc cggaagcctg gtacatctac tgaaaattgc cccgaaggca
     3001 gttgtggtcg gaagcgggca ggcagtaaaa tatctcgaag acattgtagg tcattcgttt
     3061 aagcatcaga ttgtaaaaga tggcgatacg cttgatctgg ggaatatgac ccttcggttt
     3121 atcggcgctc ctaacctgca ctggcccgat tcaatgtaca cctggctgca gcaggaaaaa
     3181 attctgttca cctgcgattc gtttggtgct cactattgcg ataaagcaat gttcgatgat
     3241 atgacagggg actataacga agcatttgat tattacttca atgtgatttt aaaaccgttc
     3301 agcaagttta tgctgaaagc gattgataaa atcagcgatt ttgatatacg catgattgca
     3361 cccggccacg gccccatact gcgcagcaca tggaaagaaa aagtgcataa atcgaaggta
     3421 ttatcagaag aatatgtggc cgagaatact cataaacgca agaaagtgtt ggtagcttat
     3481 gtttcggcct acgggtacac gcgcgaaatg gcagagctta ttgccgatgg aatccgaaaa
     3541 catgatgata ttgaagttga acttgctgat attgaaacca ttgaattggg tgagctcgac
     3601 tcaaaactgg tttctgccga tgcattactg gtgggttgcc caacactgaa ccagaacata
     3661 ttattgcctg tatataagct tttcgcgttg attaatccaa tacgtgatcg tggtaaaatg
     3721 gctgctgcat ttggatcgta cggatggagc ggcgaaggtg ttcacataat agaagctgtg
     3781 ttaaaaagtt tgaaaatgaa tgttatcagt gagccgaaac cgatgaaatt ccgcccgggt
     3841 gaacgcaaca accatgagct ttttgaatat gggaaaagct tcggtgagaa aatgaaacag
     3901 gaaattggtt aggtacacgt attttctgtt aaagctgact cactgcacac ctcgactcca
     3961 cctcgactcc accacccgtc tttgcgcgga atccacagga tgacagagca atctgaattt
     4021 acgggtggca ataacaacga aaatgtggtg ctggtatgcg gatttgtatc gccgggcatc
     4081 tcgactccac ctcgactcta ccacccgtct ttgcgcggaa tccacaggat gacaaagcaa
     4141 tctgcattta cgggtggtaa taacaactgc aatgtggtgc ttgtatgcgg atttgtatcg
     4201 ccgggcacct cgactaaacc tcgactacac cacccgtctt tgcgcggaat ccacaggatg
     4261 acaaagcaat ctgcatttac gggtggcaat aacaacgaaa atgtcgtgct ggtatgcgga
     4321 tttgtatcgc cgggcacctc gactccacct cgactactcg gtgtccggct tttgacttcg
     4381 ctttgctatc taaattaagg tagcaagtcg ggaggggatt ggttagttac ctgtaatttt
     4441 ctaaggtaat tttcaaaaga gtgccaatat tgccttttta ataaatgcca cggcaagcca
     4501 gatgctcacc gtaagcttca gcaatattcc gaaaataagg ccaataaacg atccgaaagc
     4561 ggctttcagc gatttgttaa accgttcttt acccgacaat ttcggactgt tttcgcggcg
     4621 attcaggcgc ccctgataaa taagctctcc taccagtgca ccacccaatg ccccaagaaa
     4681 tattccaacc ggagaatata ttaaaccaac aatgagtcca attacacttc cccatgcccc
     4741 ccacttacta ccaccaaact ttcgtgtgcc ccaaacgggg attagatagt cgagaactgt
     4801 cacagccacc acaacaactg cccaaacaat gagaaatgtg gagctgaaat gacaatattt
     4861 tgtgaaatga agtaataaaa taccacagta actcagagga ggtccgggta ttgcaggaac
     4921 aatacaacca atcaccccaa gcactatcag tgcaaatcca aatatcagca aagtgatgtc
     4981 gagggccatc aatctttatt ttttgtaccg gaagaagaac ctctcataat ttctctgata
     5041 acttttaaat cagcgcttaa tgtaacataa gccagatatt tatcttttga tttttttact
     5101 acctgacatg aaattccaag ctcggtgagg acagtttttt cagccgttct caacttgttg
     5161 ttataagcct catcgtttac cagggtttcc gatttataat gctctgcggt gtataaaccg
     5221 atcttctctc ccattttttg ctttgcatcg ataatggcta tttcaacagc ggtattttca
     5281 cgcgaacccg acccctgacc acatgcgtaa agcatatcgt ctgtatcgcg ataatcgctc
     5341 tcggcacaag gttgcgacgg tttacaggaa cttagcgata ctataattcc tactgcaagg
     5401 agaaaaaata tgtttttcat aaacccgaat tttattcctg ataaaagtaa taataaagca
     5461 gaagaattcc aaaaaaggat tgtagccggg ccgcatcaaa ccggccattt cacagtcgct
     5521 tcaaactcgc tgaggtagcc catccatgct tcccatcggg caattttatc tccgaccagt
     5581 tgtttagcgt gtctgtaatg gaaactttca ggccttcgtg aatttcgaaa aggttattgc
     5641 cttcgttgct gggggagctt tttatcataa catcctcgaa aacaatggca gttttcgggt
     5701 tttcagcagc ttgtttttgt ttgatactaa agaaaattgc agcagctgta gttatgagaa
     5761 atatcacagc catgctaaaa ccaagtattt ttattgatct ttttccactc agcaggtaca
     5821 ctattgcaaa aactacagtt atcataagcg accccagtcc tgcatatgcc catccgttac
     5881 tggatagcat ctgttgcaag gcattgtacc aaattatata aaacacaggc tccatcctgt
     5941 ttattttatc cggtatccgc gaattggcaa tttttaagtt tgccagggct ttttcgtgtc
     6001 gtgggtcaat ttgaagggct ttttcgtaat agtaaattgc gggagcaaac tgctctcctc
     6061 tgaaatagca attccccagg ttgtaataca attctacgga agaataattg tcatcggcca
     6121 accgctgata tatcaactgt gcttcgtaga aatcgccctt tgtgtaaaag tttttcgcac
     6181 tgtcgaggga agtggtaatg tcggctttcg caaagaaaaa aaggaaagcc aagcaaatgg
     6241 tcaaaaaaat tcgttttatc acattaacag ctttcatgct ttatttctga attttaagtt
     6301 tttgctcaaa caaattaata atgtcaacag ccctgtcgaa aatctgcagc ggttgttccg
     6361 aaccttctac gggggcaaaa tgagccagtt cacaatcttc gataatttta agaaaagtat
     6421 cggtaagttc aggttccagc cctctgtcag caagtttcag gctaacattt tcttttgaca
     6481 gttgcgcaag cggaaggttg agtttatcgc taaggtaacc ccataatgcg accattactt
     6541 ctttatagaa ctcgctcgta agattgtttt tcagcaattc attggccttt ttcagccttt
     6601 tcatggtagt tttattggct ttcctgtttt tggtgtattc aatgttggca ttttctttga
     6661 ttttttttct aagcagcatc accactacga taaaagctat cagaggaatt gatgcaaaaa
     6721 caatgaacaa cgggctccca ataaaaaatt ttctgttttt ttcaggggta aagttttctg
     6781 tttcaatgaa acggatatcc tcgctcccaa tataattgat ttcactgcgt gaatagtctg
     6841 aactaaactg tccagttgaa tcaccgggca tcttatatac atggatatta tagctattgg
     6901 ttttcagcgt tttataaatt cccgctacag ggtcgaaata ggcaaacctg atttcgttgg
     6961 tattataatc gccaggatat ctgggtatga gtgtgtattc aaaaacttta gagcctgcaa
     7021 tttctccgtc acctgagttc aggttcaatt gcacaacagg gtcgaacacc tcaaattcct
     7081 ttgggaagtt cagatcgggc gggtcgatta atttgagatt tccggttccg gaaatggtaa
     7141 ttttcattgt caaagcgtcg ttcaccttca cactgtcggc agatatactt cccgcaacgg
     7201 taaaagagcc cactgctcct gtaaaatatc ccggagcatt tccaggaagt ggtttcacag
     7261 tgattttcac ttcgggtgaa ttgatggtaa gttgtttgtt ctgataatac ccaaagaagt
     7321 tatcgtatat gctctgacct ccgtttaccc gttgcctaac cacaatatcg gcgccaacac
     7381 ttccaatgct cagggttccg cttcgttgcg gatagagaat gtcttttttg agtaccccca
     7441 ccatatagtt cgttccattc aattgaacct gttggaactg tatctgttca ggagttttaa
     7501 cctcttgcgc ccagaagcca tcaaaaccgg gaagccggaa gtcttcgaaa cccaccaaat
     7561 cgacctttgt ataaattttt atcgttgcag taattttatc gccctgataa accgatgttt
     7621 tatcgacttc ggttcggata aaaacatcat cggacttaat ttcctgggct tgtgcaggtt
     7681 gttggttgta aaatgggtcg ttaccccaaa agctttgagc gcggttggta ttctgctttg
     7741 gtttagagcc ggccttcacc actttcacag taactgcatt cgatgtataa gtttgtcctc
     7801 caaaaaccac tttgccgccg gggatggtaa acgtgccctc actgttagcc cgcaaaatat
     7861 acgaataaga ataggtgttc gattgcgaca tgttgccgtt gataatctgt acgctggtgc
     7921 ttgtacccgt tgaaggccct cccatcaggg taaatccttt gagatcgggt aactgaggcg
     7981 taagcctttg gtttaactca tatttcagat aaaattgctc tccaacctca acagtctgtg
     8041 gagcccaagc cgaaaattga atgccatcag cacaggcaac tatcgatatc catatcccga
     8101 ttgcgaaaaa aagcagtttt ttcatacaca tctttcagta atcatgcaaa attaattaaa
     8161 ttcgtcattc ttattcagaa aaccatgagt gcttatcttt tcaacatcag tttaattcag
     8221 gaacgaaata tagtaccatt gattacgatg tgcatcttaa taaaaagtta aagaatagta
     8281 aaaaaacaat taagtgaatg caaaacaaca acttccatac cagtctcata aggcttgtgg
     8341 cactaattgt ttcgggcaaa tcgttgaaag atcccattgt taccggacat gcatttgata
     8401 gttttctgaa attctatgtt cccgaccggt tgcataccga ttatcgagat aggttcaaca
     8461 gttttgtggc tgaatatgct tcagagggta agcttaaaaa gatttcatcc aattccgtga
     8521 ggataattcg catttgcagc gatataaata cacgatacaa taaaagaact aagatgcttt
     8581 ttctgatgca tctgactcaa attttatacc catatgcaca gagcgacgct gaagtaaacg
     8641 attacatcaa gctcgcttcc gccagtatga atatcgacga tcatattctt tcggtcatgc
     8701 tggaattcat ttctggaagc atgactgaaa taagcaatga tatctgcaaa gaagtgcttc
     8761 cgggtttaca ttgtttgtat ctagctgaat ctgaaataat tctgattttt acaagccaaa
     8821 gtgattatta tttaaacgat agaaaatttg ataagggaaa tgtaacagag tttgctccag
     8881 gcatgcacat tgcacggcaa ggcgaggatc cggtgtattt cagcgacata aaacgtgttt
     8941 tcgatattgc agattcgcag gaattttctt tacacgtcaa cgagttgagt tattctgttc
     9001 agggaaaaat agttcttcag ccggtaagtt tttctgtttc aaaaggggaa tttgttgcga
     9061 tccttggtaa aagcggcacc ggaaaatcaa ctttaatacg aatgctggcc ggtgagttaa
     9121 aggcatcaac agggcatttc ttcattaaaa aggaaaaaga gcccgccttt ccagccatcg
     9181 gtattgttca tcaggatgaa ttctttattc cggagttcac tgtttacgaa ctacttttgg
     9241 agcgtatgaa gctgttacag gcttcgggaa aagcagaccg gaaacaaggc acatcttctg
     9301 ttattgaagt tatggaacaa accggactga gtactattgc ttcacaggtt gcaggaacac
     9361 ctgaaaagac gcagctttca ggaggacaac gcaagaggct tgctattgct ttatgcctgc
     9421 ttcaacatcc cgaaattatg cttgttgacg aacctaccag cgggctttca tcggccgatg
     9481 cccgcgaaat tatggggtta ttacgaaata ttgcaagctg tggaaaaaca gttatttgtc
     9541 ctatacatca gccttcctac gaatatcttc attacaccga tcagtcactt attctggatg
     9601 aaggaggctt tccggtatat tttggaaaaa ccgatgcagc agctgtctat ctgagattac
     9661 atgcaggcat caccgacagc cgcgcaaccg cttgctccgg gtgcaaccgt tttgatccgg
     9721 acgacttatt ttcaattatc tcagaaaata caatcgatga atttggcaag gaaaccaatg
     9781 tgcgcaaaat tgcccctgaa acatggcacg aaatttttcg taaatctgtt gtctcaaact
     9841 atgaaaaacc cttatcccag ttaccattaa aaagaaaaac gcacaagata aattattttt
     9901 gtctgtttgc acattattta atcagagacg taaagcgcag gcttaagaac ccgatacctt
     9961 tagtattgct attactgatt actgcagctt tgggaaccct aaccggagta atttgccaca
    10021 gcggagaggt tgtatatttt tatggcgaca acccgaatat acctgctggg tttctgatga
    10081 tgataatcac tgcactgttc acaggtatga ttatggccgg gaacgaaatc cgcggtgacg
    10141 tcaactttcg taagaatgag atgcgctttt cggggaaaca gggcttgtgg cttcttacca
    10201 aactgctgcg tatgctgttc ttgtcgttgg ctttgtcggt aattcttgtc acgataacat
    10261 ttttgttgtt cgcagcaccg cttaactatg cactgcacat tgtcttgttc atgctgatct
    10321 ttttcaatgg tgcagctatg ggcctgatac tttcggcatt acttcccagg ttatcgcagg
    10381 tatatttttg tgtgccgctt cttgtggtac ctcagttgat attaagtggt gtagttatcg
    10441 attttaataa ctatccttct tttttgaaaa acaataaata tgttccggca atagcagagg
    10501 tagctgtttc gcgttgggct ttcgaggcta ttgctgtgaa tgcattcgtt aacaacgact
    10561 acgaaaaact tctgtatcgt gctcatttcc aacagcatga agcagcctat tatacgaact
    10621 attttctgcc tcagctcgaa gaaatagcag cccacgacaa ggataaagcg gcaaaactct
    10681 tagcatcgga atggtttgca aatgcaaatt tcccgaagct taaagcagat gtaaacactc
    10741 agattagctt tcttaagcaa tactacagcc accggatgaa tgaaatatat acagaacagc
    10801 aaaccatgtt cgaacagtta aacaaagttt acgacttgaa agagttgaga agaaagaaca
    10861 acaatctgtt tctggaaaag atgctcacag cgccccaaca aggacacagt ttggtggtaa
    10921 ccgaaagtat gattttacgt aaatattcgc cagtattcga cttaccggag aatgccggcg
    10981 gaagagccca tttctatgct cctgcaaaaa ggctgggaac aaaatatatg gatacattta
    11041 tattcaattc actggtgatt tcctgcatca atattattct cggacttctt cttgttgtat
    11101 tcgggatttt acattcacac aggttgaaag ccttataaca ccattaaaac ttattctatg
    11161 gacaactcca ttattacaat tgtatcacaa caaacaggca tgtctttcag gcaggttgct
    11221 gcggtggtac acctactcga tgagggggca acaattccat ttattgcccg ctataggaaa
    11281 gaagtaaccg ggaatcttaa cgaagagcag attgcactta taaaaacaga gtacgataag
    11341 aaatgtgaaa ttgcggatcg aaaatcgacc attcttgcaa ctatcgaaaa gcagggtcag
    11401 ctgaatccgg aactgaaagc ctcgatagaa agctgttggg attccaacca tcttgaagac
    11461 ctgtatcttc cttataaacc caaaaaacgc accagggcaa tgattgcacg cgaaaaaggg
    11521 ctggaaccac tcgctgtata tatcatgaag caggttcagg gagaccctgc aacagaggct
    11581 gcaaaatatc tctgcgaaaa tgttaaagac attgaagaag cattgcaggg agccatggat
    11641 ataattgcag agcaggtaaa tgaaaacaaa cctgcacgtg ataggattcg ggttcttttc
    11701 aaaaacgaag cggttatacg ttcgcaattg ataaaggcaa aaaaagagga ggctgcaaaa
    11761 tatgaagatt atttcgatta cagcgaaaag ctttccaaat gcccatcgca ccgcttactt
    11821 gcaatacgca gggcagagcg cgaaggactg ctgcacgttg atatttcgcc ggatgaaaca
    11881 aaagcatttt cgctgctggg ctctctcttt gtaaaaaaca atactccatc ggcaaatttg
    11941 gtaataatgg caatgaaaga ttcatacaag aggctgttaa aaccatctat cgaaaacgaa
    12001 tttgccgcag aaagcaagca gctggccgac attcaggcaa tcgatgtttt caggaacaac
    12061 ctacggcaat tgcttctctc tcctcctttg ggtcaaaagc gtgtactggc aattgatcct
    12121 ggctaccgca caggatgtaa ggtggtgtgc ctcgatgaac aaggtaacct cttacataac
    12181 gaaaccattt acccgcacaa acctcaggaa gaacgtgctt tgtcgtcgaa acgcatcatt
    12241 tcgatggtta atgcctataa gatagaagtt atcgctgtcg gaaacggaac cgccggaaga
    12301 gaaaccgagc aatttttaaa aaggctgagc tttgacaggg acctgaaagt gtatgtggta
    12361 agcgaagacg gagcctctgt gtactcagca tcatctgttg cccgggaaga gtttcctgaa
    12421 tatgacgtta cggtgcgtgg agctgtatca atcgggagaa gattgatgga tccgcttgcc
    12481 gagctcgtaa aaatcgaccc aaaatcgata ggcgttggac agtatcagca cgatgtggat
    12541 caaaaacttc taaaggaaag cctcgacagg gtagttgaat cgtgtgtgaa cagcgtgggc
    12601 gtaaacctaa atacagccag taaacacttg ctgacttata tttcaggact tggccctact
    12661 ctggcgggta atattgtgga atatcgtaaa aagaacgggg cttttctgac ccgcgaccag
    12721 ctgaaagagg tagcccggtt aggacccaaa gcttttgagc aggctgcagg ttttcttaga
    12781 atacgcaatg ctgcaaatcc gctggacaat acagctgttc acccagaaag ctatcctatt
    12841 gtcgaaaaaa tggcatacga tctgcattgc agtatcggag agcttattgc cgacaaaacg
    12901 cttcatacaa aaatagtaat cgggaattat gtgactgata atattgggat tcccactctg
    12961 actgacatca tgaacgaact ggaaaaaccc ggtattgacc cgaggaagac agtgaagatt
    13021 atggaattcg cttcccatat caaaaccatc gacgatcttg aggaaggaat ggaactcccc
    13081 ggaatagtta caaacattac caatttcgga gctttcgtcg atgtcggaat aaaacaaaac
    13141 ggactaattc acgtgtcgaa tatggcaaac cgtttcatta aagacccaaa cgaggtggtt
    13201 tcgctgcacc agcatgtgca agttaccata atcggaattg ataaaaccag ggggagaata
    13261 caactatcgc taaaaaaatc agattcctaa ccggctgttg tccgtaaaca ctgcaaacaa
    13321 ttaagctgtt atttcgtaag cccgtaaata aatttccgtg ccagatcgaa aggtatgata
    13381 gaaaccgata acagaaggat aataagccat tcctgaatgg taagcgaatg ggtgcgtaag
    13441 aaatcgccac ctatatatgt aaaaactatc tgcataacaa atataaagcc tattatcggc
    13501 agaaagccct tgtttttaaa gatattctcc atcaggttca ttttctcggt tcttgcgtta
    13561 aaactgttga acataatcag gaaaataaac agattgaaga aagcggtcat gaaaaccata
    13621 gtatcgggtg ccccgtttct aataaagagg tctttaaatg gcgggaacaa taggaaaaaa
    13681 acagcaaaag cagtaatata aatacctccg ctaaacatag ccgacgacat agctttagaa
    13741 acaatatttg catcgcgacg gataggtttt tcattcataa accgctgcag tgcaggctcc
    13801 ccaccaagtg ccaaagcagc aagtgtgtcc atgataatat ttatccagag tatttgtata
    13861 attgtcaagg gaaagtcaat tcccaacagg ggaccaagaa aagaagtcag cacagcagca
    13921 atattaaccg ttagctgaaa aacaataaac ttacgtatcg atttaaaaat ggttcttcca
    13981 tagcgtattg catttgtgat agaagagaaa ttgtcgtcga gaataacaat atctcccgat
    14041 tctttggcta cttctgagcc gctgcccatt gcaaaaccca catcggcctg tttcagggca
    14101 gctgaatcgt tcaccccgtc acctgtcatt ccaactactt taccgaggtt ttgggcaata
    14161 cgtaccagac gacttttatc ggtaggcaat gcgcgggcaa caaccctgag atgaggcagg
    14221 atttcttcga cctgctcatc ggtgagctga cgcaaatcct gagaagtgag caccacatct
    14281 tcatccgatg ccagcaagcc ggtttcttta gcaatggcag cagcggtacc tttccggtca
    14341 ccggtaatca tcactacctg tattcctgct ttcattgcct gaataatggc atccctcgat
    14401 tcgggacgaa cttcatcttt aataccgata atacccagca gagccatccc ttccggcaaa
    14461 tcaccctcct cttcaatagg attgtcggag catgccagtg caatgaggcg aataccctga
    14521 tttgccattt cgtcggacat ttcaatgaga tttcttttgg agcctatatc acacaagtgt
    14581 ccttcagcat taaatatttt tgtgcatttc tccattaaaa tttcaaccgc acctttaaca
    14641 aaagtaactt tgtcggatag aaaaatatct ttgagtgcgg catcgccttt taccagagta
    14701 gctgagaatt tctttgcact gttaaaccgt atatttgcca atgcctgagt cgaaggatgt
    14761 tcattcaacc tgtcttcgcg gctgacaaaa cggataagag catgttccga agcattgcca
    14821 cctacaggtt ctccataacc atcgagtacg caataggaat tatcgaccag agaaatccga
    14881 accatgttac gaagagcagt agggatatcg gaataattat tgaaagtttc gcccttacca
    14941 tcaatcatga atattgggtc gagccttccc tttgtgatgg tacccgtttt atcggaaaac
    15001 aaaatattaa tactgcctgc ggtttcgata cccaacagtt ttcgtaccaa aactttctcc
    15061 gacagcattt tgcgcatatt cagcgacaac acaatggcaa tcatcattgg caaaccctca
    15121 ggaactgcag ccacaataat tattatagcc agcaccatgg catgtataat gtcgttaaca
    15181 accacgtccc agtgctcaaa atacgcagtt atcaaaccca tatcaaaatt gtgaagaata
    15241 aatattttgt tgaaaacaaa agccacaaat atcaaaccgg ctccaatgta accgaatttg
    15301 ctgatgagtt ttgcaaggtt tttcagtttc atttgcagcg gtgaaaggcg gtcatcgctc
    15361 agtgataact ccactgtaag ttgtccataa aagcttgcag ctcctactgc ttctacaaga
    15421 ataactgctt caccttcttc caccacagaa ccacgaaaaa caaaatggcg atcttcgagg
    15481 tcggaagctt caggctcata atcggcctca gccggagttt ttaaaattgc aacagcctca
    15541 ccagttaacg atgcctgatt cacatgcaat tccccgttga caagccttcc atcagcagga
    15601 actttatcgc cgctttgcag caacacataa tcacccttta caacctcatt aatagggata
    15661 ctctgaatgc gcccatcacg aaatacgtta ttgtatatca acgaagcttc ctcctgcaac
    15721 ttctgaaaag agttttcgtt cttgtactct gaaaaggtgg aaacgaatac cgacagtagt
    15781 acagcaatgg ctattcctac ggcttcgaac cattcagtca aaccgaaaaa tgaaagaaca
    15841 actattacta ccaatgctgc gagtaaaata attataatag ggtctttgaa gttcccaagc
    15901 attttaagcc ataatccttc gccttcgggt ttcgctattt cgttgctgcc attttctttt
    15961 ctactgcgca gcacctcttc ctgagtaagc ccattaaaaa cgtatttcat agttctttat
    16021 aataaattaa atactgttgg ttttattgat attactgcat tttacaataa ttctgatata
    16081 ttaattttta caaaattatt tttgattaaa cggtcagtca aaaataaata gtttttttca
    16141 aattcaaaat attttaacaa ttttttttat cacaggctta atgattttgc aacactttaa
    16201 aaaccgacaa tgtttgcata tttacgatat aaatattttt ataaaaaaaa ttcaatattt
    16261 cgttatatgc gaacccttct tcagccattc taacagcaga ttcctgcgac aatcctactc
    16321 catgaccgta tcctctgcca gaaacagtta tcgctgcatc attcatactc atactaaaaa
    16381 aagcagaagg gaatctgaaa taatgacgta tttctgtcat agggattgaa acccctccaa
    16441 ttgtgactct ggcaatacgc gtacctgagc tgataattga agaatcaacc gggattgaag
    16501 catcaacgcc tttggtctta agaaacgact tccattgatc tgccgtgatt gtctgctgcc
    16561 aaacagcatt ccgaccataa acgtagtatt tatcttcaac gggtttcaaa taactcaggg
    16621 aacttatcca cacatcctcc gaattcatag taatgcctcc gctattggaa tggtatgctg
    16681 ccataattag agcatagttt tcatcaacaa ttacgagtcc tgatgtggct tgcacagcgc
    16741 gtaaaatatc agggctttcc gatttgcttt tataagcctg acagtgagtg ccatcgcaca
    16801 gttcaaaacc ctcagcctga tgcttgccga tattttttaa agcaaatgtg cgtgaaataa
    16861 ccgcttgtgc tttatagaat tcaaatggtt ttttgtagcc tccttcagct tcggtaactg
    16921 cggcaacata gttttcgagg gggacattat ttaccagtaa cagattctga ttatttttgc
    16981 gaacatacag attgccatca tatatccgtg cacttcctac tgcaggcaca agcgttactg
    17041 agaagcaacc ctgagtttcg gtttttattg aaatattacc gtagaaaata tggctgctgt
    17101 cgtttgcctg taccataata ttaccggaaa acaggcttat aaaaacatcc tgccctggag
    17161 tcagggcaac aacagtagaa tcgttttcgg ccagcgcgta gcaccctgaa atgcttttaa
    17221 aaatcacctt attgggtctg tcgttgtaaa atatggcaac attaagattc tgagccatca
    17281 taaaatgccc tgacataagg attacaagta aaatgctaat gcgagccatc actcatcagt
    17341 ttaacaattt gcgctccaac acgtacagtt gaaccgggat ctcccagtcg attacgcaag
    17401 tctgcatagt cgttaagcat ctgcttacgc ctggctgtat caggaagcag ggaatcaatc
    17461 tcttttttca gattgtttct gttcaaccga aactgtagta attcagtaac acaagcttta
    17521 ttcatgatca ggtttacaag cgatatatat ttcacttttt tcaccatggc tgcaataagc
    17581 acagaaatta aaccggcttt ataacatact gcctgtggca catcgagcaa agcagcttca
    17641 agagtggctg tacccgattt gatcagtcca atttcggcat gcgacagcac atcgtaggtt
    17701 ttattcataa ccaatacagc gcctgcttgc ctcaagtatt gcgaataata gtccttatca
    17761 atacccgggg aacccgcaac aacaaactga tactgaggaa aatcatctac taccgaaagc
    17821 ataatgggca acatccgtga aatttcctgt ttacgactac cggccaataa tgcaataaca
    17881 ggcctgccat caagtgaatt ttctgcacag aattcattaa aagtgatttt cctgtgactc
    17941 tcaacagcat caatcaaggg atggccttcg taaacaacgt caatattgta gcggctgtaa
    18001 aactcctgtt caaacggaag tattacaaat agtttttcgc aatacttttt aaaatggaac
    18061 accctgttcg atttccacgc ccacacattg ggtgaaatat agtagtacac tttaatgccc
    18121 tgcatttttg cccatttggc catgcgcata ttaaaacccg ggtaatcgac gagcaacagt
    18181 gcatcgggtt gatattcaat tatctgcaat ttaacaaggt cgaaatttcg tagaattgtt
    18241 ctcaggtttg caacaacttc ggtaaaaccc ataaaagcaa gttccctgat atgttttaac
    18301 agatgaagtc cgcctgcctg cattaggtcg cctccccaac ccgtaaactg agcttcggca
    18361 tcatattgcc ggatgccttt catcatattg gaagcatgca aatcgcccga cgcctcccct
    18421 gcaatgataa agtacttcat ttaagcaaac ttaagtacaa ccacagccac cgtgtagagg
    18481 aagaccgata tcacaacacc cttaattgcg taccagtaat tgagtttttt aaatataaaa
    18541 aaaagtatca ggttgggtaa aacgcaaagg ctcataaccg gcaataaagc atgggtattc
    18601 ttcaggtgct gaatgtaagc tgaaaacgaa ggtattacat tcacagcaaa cattattaaa
    18661 aaagttatca aagggaatac cagtccaagt ccaagtccta ttacaggctt attcagtttt
    18721 tgtagtttgt cgcttatcat atgatttata atttccagtt cctgataata tcaatggttt
    18781 tgtgcgaagt gaggtcggca tgtaccggca caaccgaggc atagttgttt ttcagagccc
    18841 actcatcagt atcttcagca cagtgctcaa agttctcgaa gtttccggag agccagtaat
    18901 atttcatccc ataaggatcg gtgtgttcca caaattcttc gcgccatacg cccttagcct
    18961 gcctgcagat tttaaaccct ctgaagtcgt ccggatcaat attcggaaaa ttaacattaa
    19021 aggctacgtt tttaggcaat ccattcgcca gcactgcatc aatcacttta cccccatttt
    19081 tctgaacaat actaaaattt gcatcggggg cgtgattgag aaccgaaaaa ccaatagagg
    19141 gaatatcgta caacgaagcc tcgatggctg cgccaatggt tccggaatag attacactga
    19201 cggctgaatt cgatccatgg ttgattcccg ataccataag gtcaggctta cggggagcca
    19261 agcggttaag tgccatcttc acacaatcga caggcgttcc gggtaccgag tagaacatca
    19321 tgccctcttc tttatgaaca aggtttgccc ggagtggttt taaaatgctg atggaatgcg
    19381 acatgcccga ctgatgattc tcgggtgcaa ccacaagaag ctctccataa gggcggacca
    19441 tttcaatgag gtgcttcaga ccttttgcat tgataccgtc atcattcgaa acaagaataa
    19501 gcttcttttg catttccgat atatttcatt cgtttttccg gctgcaaaga tagtgatttt
    19561 ttgtaaaagt aatgttgcta aatactgccc ttaaaagaga atgtttcgcc caaggtctat
    19621 tttgggttac ggctgctgcg cttgctcagg ttcatcagtc ccagcataaa gaacacaact
    19681 atcagaatac ctgaaattac cagcgacatg cgcgcgagaa aatcaccata gcgggtgtaa
    19741 aaagtcagtg tatcgttagc atttactgtt gcacgaataa catccttctt ccaccattcg
    19801 gttgcttgca gaatatcgcc gcgttgattt acgaaacaac tgatacctgt attggcggaa
    19861 cgtgcaatac accgacgcgt ttctacagca cgcagcgacg aaaagctttt atgctgacga
    19921 tgccctgctg tgttacccca ccagccgtcg ttggtaatga cgcacataat tccggctcct
    19981 tcctttacaa atcctgcaaa aaattctcca tatgccgatt catagcatat cgcggtaccg
    20041 attttcaacc catctgattt ttgcatgggc gtcctttctg cctgaacacc atggcttccc
    20101 gacatgccac caaggtcaat tgcaaaatca ccgaggaatc cgagaagttt ggggtatggc
    20161 atcatttcga ccccgggcac caacttgctt ttatgatatg cttgtatagc cggggaagta
    20221 tctatcagca gcgcagtgtt gtagaggtcg taataatttc cggttttacc catagcaatg
    20281 gctgtttccg atttccccac cccatcggga taataatcgc gcgtatcggc acccattata
    20341 aatactgcct ttgggtagtg ctgcagataa gctttcaggt agcgaatggt tctttcgtca
    20401 tccatttcgc tctcccacat tccatcggga attgccgttt cgggaccgac tatcacatcc
    20461 acggtgctgt ccattttctg gtcggcaagg gtaagcatgt cgctgagctg gtccatagaa
    20521 ggtgtctcaa acttttcgtt atacggatcg atattgggtt gaataacaac aatatccacc
    20581 ggatcaggtt tttcggagta ggtgtagtaa cgcaccacag aatatcctat cgggacaagg
    20641 atgagtaata tcagtgatat cagattttta cggttaaccg gtttcttttt cagtaaaaac
    20701 atatcacgaa caaaaagaaa aatcagaaca ttcagcagca atacccataa ggaacctccc
    20761 agatgaccgg taaattcgta ccactgaata agcgtaatat tatttgcaaa agcgttgccc
    20821 aaagtcagcc acggccatga tatctgactg ttcatgaaaa ggtattcaaa cgatagccag
    20881 aacgatacaa atgcaagcaa accaatgcgg ggtcccattt tccgttttat tattgccaca
    20941 ctccagaaaa cacatgccat aaacagcgaa agaaaaaaga ttgccgctat ggcaccaaac
    21001 acggttgagt agtatatcca gtaggtagtt attgcgttcc atatcagaaa agatagaaac
    21061 gaccacaaga aactgttcac tgtgcgaaat cgctgcctgt tgatgtaatc gtgctcttcg
    21121 ataatgagca ggggaacaaa ggctatccat accaaaggac ccagacccca ttgcgaccag
    21181 ccgcagcaga aaagcaatgc cgatatcagg ctaagcatca atttatagta ccatttcagt
    21241 ttcatttcat tcgcttttat ttaccagata aactccggct aaaactgtga agatagaaaa
    21301 tatttgaaca cctgtaatac tttcgccgtc ggcaatgccc caaaagattg caaaaacggg
    21361 gataacatac gttaccgaag aggcaaacaa tgctccggct ttctgtatga gcatgttcat
    21421 aaaaattaca gctatcaccg accctgctat tgatagaatt acaaggtaaa tcaagctcat
    21481 cgtaaaatgc ggatttgcca ccgtttccgt cgggtccgaa aatatcaagg ctgcaatgct
    21541 gggtggaaac acaaacaaat acgccagcga agtgatctca aatccggtga aaccgctgag
    21601 gaatttcttt acctgattta cattgtagcc atacatacag gtagcaacaa caataagcag
    21661 tgcataggca ttaaaagtca tgtccgaaaa accgtttctc atcaccagac caaatgctcc
    21721 ggccaaacca atggcaatac cgacaatttg cttccagtag gctttatgcc tgaaaaacat
    21781 taacgcaaaa atgagggcaa aaaaaggcgt ggtcgcatta agcattcctg ccaggctact
    21841 attaacccga gtttgtgcaa acgaaaataa aaaagcagga atcaggttcc caataaatgc
    21901 cactaccata aaaggaacaa tgttgctgcg gtttatctta tttagtaatc gtaacccaaa
    21961 tggcagcgtt accaatgaag ccaacgacat ccgtatagct gccacctgaa caggtgtaaa
    22021 aacatttagc ccctttttca tgagaatgaa ggaagcgccc cagataaatc cgagcagaat
    22081 taatataatc cagtgataat atttttgtgt aaaagaaata gaaatttggt attaaatttg
    22141 aattccggca aaattataaa ttctcgtgat gaaaattatt aaatttatca ttgtgcttca
    22201 tgcaatactg tttttttcgc tacgtgaagc caatgcacag atgccacgat atatcggtcg
    22261 gttaatcaac atcgacagta ttgctcaaaa tgtgggtatg ccggcatttt tattgcaaga
    22321 agtatgtgtg tttccgaaag accagtataa ccgccagaag cgctacaaca aaatggcacg
    22381 taatttttta gtggtttacc caattgcact ccaaataaaa gatgaattcg tgcagattga
    22441 ggaagacatg aaagcaatga atccacgcag tcagcggaaa tatatggata aaaaagacaa
    22501 cgaattaaaa caaaaataca agaagaccct gatgaaactc accctgagcc aaagcatgat
    22561 gctggtaaaa ctggtcgatc gcgaaacggg taatacatca tacgagctca ttaaagagct
    22621 gcgtggatca atcactgcat tcttctggca aaatatagcc cttctattta aaaataacct
    22681 taaaatgcac tacgaccctg aggtcagcga caaggaaatc gaaatgctgg taaaacgata
    22741 cgaagcgggg acattgtaaa aataaaacta aagacagtct gatagattat tctgccggat
    22801 tacaatttac ttcaagaagt aggttgcact aagaataaag ttattgttgc tacgagtccc
    22861 tccatggaaa tagtaaaaat caatatatat atcccaatcc aatgggacat aatcataata
    22921 aaaaccccgg attccccatt ctgaaatcaa gttcaccgac aaattcggcg taacataaaa
    22981 cgacattccg aggcttattt gacttgtcaa cctgtttaac gacaactgtg tttcgttttt
    23041 atttgcagga ttataatcaa ttacggtata acgatttttt tcatcataat cgggaactat
    23101 gtaatcacat ctgtaataat cgcgggctgt aatcacaacg ttcaaattat aacctgcaaa
    23161 taatgaaaat ttgaagtctt taatattgag taacaaattt aagcgcagcg gcatagatat
    23221 tttatgaaat atatccattt cccaaacctg tcttgtgtat tcataagtat tttctccttc
    23281 cataaaacta ccatacgaaa ttgcatcacg tttcgatgcc acaaacatat ag
//
LOCUS       DAOOQP010000035        22904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2741_length_22904_cov_5.174800, whole genome shotgun sequence.
ACCESSION   DAOOQP010000035 DAOOQP010000000
VERSION     DAOOQP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22904
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2741_length_22904_cov_5.174800"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2374)
                     /locus_tag="PLK75_05495"
     CDS             complement(<1..2374)
                     /locus_tag="PLK75_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38736.1"
                     /translation="MLRFYKIVFLLLSMLICWSNALPQFYSGHQMNFGKNRVQYGNFD
                     WNFYRYNPFDTYFYSGGQANAIQVAQIANKVIPELEKFLDYKIEQRLIFVVYNNLSDF
                     RQSNIGLNTGDSQYNIGGVTRIVDNIVFLYAEGDSREMEIQIRGAIAEVMLNEMLYGS
                     GFTSKIANNTLISIPDWFFSGLVSYISNKWDYEIDNYVKDGILSGKFEQFNHLSGKDA
                     QFAGHSIWYFVANNYGENVIPTIVYLTRVTKNVENGFQYVLGNNLKMLAYDWLNYYQA
                     LYAEHDLSCDFNAKKNILKPRFGKWELIQTEISDNGNYIAYSIKNMGKYKIVLYETAS
                     GKTKTILTKEHKLEQIVDYSYPVISWHPTGKLLGYIIESNGEVFYCTYNLETKKTLYR
                     EIALLQKVLSFSYSHDGLKLVMSGYKNGMSDIYIFNIVANTITNMTDDIADDYSPQFI
                     EKSSKILFSSNRASDTLVSGKTNVVPMQICSDIFIMDADGKNNVLKRVTKTKDINEAK
                     PLSIKTNEFWYLANDNGIYNRYYARYDSVISYIDTATHYRYITRSFPVTNYHRNIGSY
                     DVAARYPSVVESVFENDRWGIYFSEISRTPFTGNLTDTRFRTVDRENLNFIPQPKPEK
                     PVKPIQHNELENIDINNYSFSQEVLQTNRKESGDFDSLGVPLRKFQQIYVTTFYTNYV
                     VNQIDFGFLNSSYQQFTGSAFYFNPGFNILFKLGAKDLFEDYRITGGVRFSANFDSNE
                     YLLSFEDLKKRLDKQYIFHRMTINSSNGYNIIKSNSHELLYVLRYPFNQVS"
     gene            2442..2936
                     /locus_tag="PLK75_05500"
     CDS             2442..2936
                     /locus_tag="PLK75_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOY38737.1"
                     /translation="MAVLKIISGGQTGADRAALDFAMSHNLDCGGYCPKGRCAEDGVI
                     DLKYPLTESPSSFYQERTRLNIIHSEATALFIVDGVFGKGSLFTKKQCLTNRKPLLIV
                     DLTDNAANLVAGFTKWISENKISILNIAGNREGTSPGIYRLTTEFLMLVHQNTEVFCN
                     EKTS"
     gene            2920..3777
                     /locus_tag="PLK75_05505"
     CDS             2920..3777
                     /locus_tag="PLK75_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOY38738.1"
                     /translation="MKKPLEISILFFLITIMELMGQNSLFSQQVPLPYELRPAREAMF
                     LSIGIPLTVVPFYVKSTTKFLDQSEIEQLNADDVNIFDRWVIDNWSLPLRNTSRMLPY
                     VSGGIGATTLFIVPAFTDQSRPYLSYVGTLSLMYIEGVLISQGLSFMMNTYINRPKPY
                     VYNTKLPMEFRVFQGNNASFYSKRACFTFYNALFSTMVMYDMFPDKPVWLYVGAGLFG
                     VAGLTVAAELSSGEHFYTDVLTGMAIGTATAYAIVKYHRVTNLNQRLTIIPAIGPKTA
                     GIYLNYRFY"
     gene            3863..7138
                     /gene="secA"
                     /locus_tag="PLK75_05510"
     CDS             3863..7138
                     /gene="secA"
                     /locus_tag="PLK75_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOY38739.1"
                     /translation="MAFLSFLTKMFGTKAERDLRETQPLVDSILSVEPEIAKLSNDEL
                     RALTFKIRAEIREHIQDEQNQINELKQKIETLEINEREALFDEIDKIEDVITAKIEEI
                     LLEKIPEAFCIVKETARRFTENEHVRATALDYDRDLAATVDFVNIEGDQAVWSNSWIA
                     GGNLTKWEMIHYDVQLFGGVVLHKGNIAEMATGEGKTLVATLPVFLNALPGRGVHLVT
                     VNDYLAKRDSEWMGPLFQFHGLTVDCIDKHEPNSEARRKAYLCDITYGTNNEFGFDYL
                     RDNMAISLSDLVQRGHNYAIVDEVDSVLIDDARTPLIISGPTPRGNDQLFDNFKPQVM
                     KLYSAQRDLVNQILVEAKKLIKDNERDKAGFKLLQAYKGLPKNKALIKFLSEEGMKVL
                     MQKTENYYMQDNSKQMHKVTDDLYFIIDEKQNTIELTDKGIDLLSQSNDDSSFFILPD
                     IGSEVANIEKLGLNDEQKLEKKDALIRDYTIKSERVHTVNQLLKAYTLFEKDTEYVVI
                     DNKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYFRMYKKL
                     AGMTGTAETEAGEFWDIYKLDVVVIPTNKPISRHDDEDMVFKTKREKYNAVINEIVDL
                     TEAGRPVLVGTTSVEISELLSRMLKLKGINHNVLNAKLHKKEADIVAEAGRSKTVTIA
                     TNMAGRGTDIKLSKEVKEAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGSSQFFVSL
                     EDDLMRLFGSERIAGLMDRMGLKEGEVIQHSMISKSIERAQKKVEENNFGIRKRLLEY
                     DDVMNAQRTVIYKKRKNALDGERLGVDIANMMYDICSGFTEEFYGSSDYEAFNFEMIK
                     VFSIEAPVTKAEFSKISHDDLTEIVFQKVIETHKRKTKTIAEQAYPVIKHVFENQGSM
                     YTNIIVPVTDGINTYKVIVNLEKAYNSEGKEICSAYEKSLVLSIIDDAWKEHLREMDD
                     LRQSVQSASYEQKDPLLIYKLESFNLFKTLIEKVNRSILSTLMKGYIPISDPGQVSEA
                     RQQRRLDTSRMTAGRSEQAYNDPSKGKPSNQPVKVEKKAGRNDPCPCGSGKKYKHCHG
                     AGV"
     gene            7208..8350
                     /locus_tag="PLK75_05515"
     CDS             7208..8350
                     /locus_tag="PLK75_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38740.1"
                     /translation="MKKLSLLLLVISGSMYCYSQNNINDSIATYIDTTQIIIPLKIGI
                     DSVAAISDTSSTLVLAENQEAELAYNDGVMLFQNGDYNTAIYKFTEAIKLRNNFDKAY
                     FNRASAHMQLQEFDLAYNDFDSCLILNPEKISALYYMGYISFYRQDYSKAAGFFDMAI
                     AKGFSDANMYYLKGIMSFNNKSFEEAITNFSSSIKIDGNNFMPYHDRGSVYRLTDKNE
                     QAISDYSKAIELNPKCYQAFNNRGSVYRMMKDYDKAIADYDKAIQLKEDYYIAINNRG
                     VAKYETGKYEEAIDDFDEVLRIKKSYAFAYNNKGLAQYRLKQYAPAVESYTKAIQYDP
                     NYGFAYLHRGSAQEMLRKFDEACADWKKAGELGVDAAKNYVGICNE"
     gene            8364..10445
                     /locus_tag="PLK75_05520"
     CDS             8364..10445
                     /locus_tag="PLK75_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY38741.1"
                     /translation="MKYRICFIGVLLAISSYLTGQEKVEILKDEFKTPKEGFKDAWDA
                     IKEGDKLFDFGEAGFRNALYPYLAAYQYNQKNAELNYKIGICYLYSVEKTKAITHFVD
                     AVKLKPNVAGDIDYFLGRAYHLTYQFDTAMYYFQSYKRKAAAKGVNITDKDVDKRIDE
                     CKYAKQYVASPIRVFIDNLGDNINSSYPDYAPVITADESVIVYCSRRPGGVGDNIDPN
                     DLQYYEDLYTSENIKGRGWSPAINMSNLNTEYHDASVGISPDGQIMFTYKGVPDGTIF
                     QSELKGLSWGKPKELPSQINTKYDESDASLTYDMKYLYFVSNRPEDGFKTPTFGGKDI
                     FITNVDAKGNWLPCKNIGGIINTKYDERGVFLHPDGNTMYFSSQGHESMGGFDIFYSE
                     KDSLGKWGKPVNIGYPVNTPDDDVFFVAAGNARFGYYSSSREGGRGYQDIYRITFLGP
                     EKLMVCGSEDNLLAGSEMAVQQKVDVQQTVEVRKVRLTILKGTITDYLSGEPVEAEIE
                     IVDNEKNEVISVIKSNSSTGKYLVSLPSGKNYGIAVKAPDYLFYSENFDIPYSQDYQE
                     VTKDIVLSKVTVGSKIILKNIFFDYDKATIRPESENELTRLVTLLNAYPKMRIEIGGH
                     TDSHGSLQYNTKLSSDRAKAVVDYLVSKGIDASRLEYKGYAFNEPIATNDTDEGRQQN
                     RRVEFKVLSIK"
     gene            10452..12440
                     /locus_tag="PLK75_05525"
     CDS             10452..12440
                     /locus_tag="PLK75_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY38742.1"
                     /translation="MNKTIISLLIAVFVNTALNAQTDVKFKSSNFSNNPKGFQNAVNN
                     INSGDIIIEEKRNYREALKYYLQAQDFNPDCAELNYKIGVCYYHSEKKHMCLPYFEKA
                     YLLDQKVNKDICYYLGRAYHLNYQFEKALTFFDMFKNSRGVTSDLLKLTPRHIEDCNN
                     AMTLILDTLQVEIVNLGPSVNTQYPEYNPLITADGEEMFFISRREGTTGNKTDINDGM
                     YCEDVYISDLVNINWTNAKNLGKPINTAKHDAVLGIAPDGQQLLTYIDINEGDIGISV
                     LHGSKWSKPELLNTNINTQYHESKACLSPDGKQLFFISNNPKNSIGGRDIFVSKLGKN
                     GDWGPAENLGKTINTKFNEEGVFIHPDGRTMYFSSEGHNSMGGYDIFKSVRDSLGNWS
                     KPVNIGFPINTPGDDVFYTVSASGKKAYFASYREDGYGMYDIYEIRFKEKKPDAIATN
                     EPEKVLVTVLKGTVKDSKTLIPIEAKIEIIDNVKNEVVATFYSNSATGKYLVTLPSGR
                     NYGINVSHDSYLFHSENFDIADTTDYQEIVKDILLQKIEVGTKVVLRNIFFDYDKATL
                     RPESAAELDKLIAILMKEPMLRIEISGHTDNKGSHEYNKKLSEGRAKTVVDYLVEHGI
                     KRDRLEYAGYAFDQPISDNDTEEGRQLNRRVEFKVLSR"
     gene            12578..13774
                     /locus_tag="PLK75_05530"
     CDS             12578..13774
                     /locus_tag="PLK75_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY38743.1"
                     /translation="MRSNVLRTILTVLIIAVGIMALVGILTAVDAIKNTISSEFTNMG
                     ASTFTIVKKRMHTRGGENELRQTAIRDITYDEARRFKNSFSFPARVSVIVDATWTATV
                     KYENEKTNPNIQVMGSDEDYLFTSGNEIGLGRNFSPNEVQSGSNVVIIGNAIAEDIFK
                     NKQSPIDKYINIGNGRYLVIGVLKSKGTSFGGAGDKFCIIPLTNARQNFTDYDLSFDI
                     SVLPYDAGKLDLAVSEAEGLFRNIRKLTVYDENDFEIRKSDSLANMLIENISVVTIGA
                     TIIGLITLLGATIGLMNIMLVSVTERTREIGIRKALGAPSRVIKQQFLFESVVIGQVG
                     GILGIVLGIVAGNLVSTATGGSFIIPWMWILLGVLLCFIVGVISGYFPAVKAAKLDPI
                     VALHHE"
     gene            13873..14406
                     /locus_tag="PLK75_05535"
     CDS             13873..14406
                     /locus_tag="PLK75_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOY38744.1"
                     /translation="MRKLRIEELNRINVEQFKNIAKIPLVIVLDNIRSHHNTGAVFRT
                     ADAFLIERLLLCGITGSPPHRDIYKTALGACESVEWKYYESTLVAVKELKQNNYVLLA
                     LEQAEGSIPLSSFQAYPHLKYALIIGHEVRGVQQEVLDLCDMCLEIPQHGTKHSLNVS
                     VSTSIAIWELTNKLSKA"
     gene            14403..15002
                     /locus_tag="PLK75_05540"
     CDS             14403..15002
                     /locus_tag="PLK75_05540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY38745.1"
                     /translation="MSETWDNRYSTSTFVYGTEPNVFFKEVVLQNTPGKILLPADGEG
                     RNSVYAASIGYDVHAFDFSLVAIEKARQLATARNVTVKYKHADFNVADKLYPDNSFDY
                     LALIYAHFPPEIRKLHHHMLLRKLKPGGKLIFEAFSKKQMPRTSGGPKNEELLYLTDD
                     LKHDFGSLSKLSVTENEISLNEGELHKGAADVIRIQGVK"
     gene            15086..15883
                     /locus_tag="PLK75_05545"
     CDS             15086..15883
                     /locus_tag="PLK75_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY38746.1"
                     /translation="MFIEGLTAWGLDAWIIILILVVSGFVVGVINTVAGSGTVISYSL
                     FMALGMPSPLANGTVRLSVVMQTLTSSLSFYRNKKLDIKKGLILGIPIVIGSIAGAQI
                     AASINKDLFEKIVGISLLILLFFIFYNPEKWVKGSLEKQQRKVSAWQYLVYLFIGIYG
                     GFLHIGVGIFLLSALVLLSGYDLVRANALKVFLVFLYSPVALAIFIMNGQVEFLLGSI
                     VAVGNIFGGWVGSRIAIKRGSGFIRILLVIIIAIFSAYLLGLWDLVF"
     gene            complement(15880..17112)
                     /locus_tag="PLK75_05550"
     CDS             complement(15880..17112)
                     /locus_tag="PLK75_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOY38747.1"
                     /translation="MVLNYIWIAFFIIAFVVALVKLLFFGDTVVFTSIMNSTFDMAKL
                     GFELSLGLTGVMTLWLGIMKIGENGGAIRILARLIGPFFNKIFPEIPKNHPVHGSMVM
                     NVAANMLGLDNAATPLGLKAMAELQELNPRKDTASNAQIMFLVLNTSGLTIIPISIMV
                     YRAQMGAADPSDIFIPILITTFISTLAGLIAVSVVQKINLFSKTILLYLGCLSVFIAA
                     IIFYFSTLSSENINTVSSIAGNLILFTIIISFIALALRKKVNVYDSFIEGAKEGFSTA
                     IKIIPYLIAILVGIGVFRASGAMDFIIYGIAWVFEAMGVNSDFVPSLPVALMKPLSGS
                     GARGLMVDAMNQYGADSFVGRLSCVFQGATDTTFYILAVYFGSVSIRKTRYALTCGLI
                     ADFAGIIAAIVVAYLFFH"
     gene            complement(17211..18410)
                     /locus_tag="PLK75_05555"
     CDS             complement(17211..18410)
                     /locus_tag="PLK75_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38748.1"
                     /translation="MMKLIISTILLLISLNASFGQSKADYYFRVLSDFEKGHTSAALA
                     LSDSLVGIFPEDAVFIRLNAGLYFKTGNYLKSQRMYALLIPYDQATARLNMARCLALV
                     NDVEAAINELKEFLTLPEVFNIADVKADTCFRYIFASKEWSEFWRGFEPDSEKLVLAE
                     LEYLLKQKEFTEVLHDNNSFSNSRYTYMAELMKTDALIELKQYKEALKKVNGILKDTL
                     SYELLLRRAEVFSAMNEYIKAVIDISDVIELNPYEIDYYLQRAVASNNAQIYEQAISD
                     LRYYLRFFEDHEEVWYKLSVVLLNAGINSEAVDVLSKLIAMNSGNELYFLLRAETYSN
                     LTNYTGAVSDYLMALDLNPRNADTYISLGWCKHRLDDNAKACYYWHKAAHLGHPEAEF
                     LLQQYCK"
     gene            complement(18407..21055)
                     /locus_tag="PLK75_05560"
     CDS             complement(18407..21055)
                     /locus_tag="PLK75_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY38749.1"
                     /translation="MAKYVVTLKVISMMKTIYSILLIVCIAVTNAFATHNRAGEITYR
                     QLTDLTFEVTIITYTATGPGNTADRPQLEILWGDNTGDILPRVEEVFLPDYYKRNRYV
                     GVHTYPGPGIYTLVVEDPNRNFGVENIPNSVNTLFAISTTMQINAALGSNSTPVLTQP
                     PVDKAAVGQLFIHNPGAFDPDGDSLAYKLTICRGENGEPIEGYTFPEASNSLTMNELT
                     GDLVWNTPMYTGVYNIAMIIEEWRKGIKIGEIIRDMQIEVYDTDNHAPQINAQEVICI
                     LAGTKLEMPFTVTDDDGDSLSITANGGPFIMTEGAAIIESEYTGNGTATGLFTWQTVC
                     EHVRRQPYIVNIKAEDDNSEVHLVYIKNISIYVVGPAVENVNISSGNDFITLSWTPDA
                     CYNVTGYNVYRRINPSGFVPDSCEVGVPEETGYTLVKTVAGHTTTTYIDDNDGVGLSQ
                     GFVYCYIITPVFPDGIEGYASEEVCTQLVRGIPTITNVSVLETSVDEGRIYLAWSKPT
                     EFDAIAAPGPYKYLIYRSEGLFGENLERIDSLAGINDTIYYDSLLNTLENPYSYRIEF
                     YNDEEGNRFLIGTPHIASSVFLRIESDDNALVLHFDKNIPWVNTEYTVYKQSPETMLF
                     DSLDITGNDYYRDTQLDNGTQYCYFVRSKGSYGIPGFVNPIINLSQENCGTPIDTVPP
                     CPPVLTAVSVCDSLFNRLSWFIPDSCLIDVAYFEVYYSPVLDGIENKVYSSANHDTAF
                     FRHFPDETMAGCYTVVAVDSFGNKSLPSNRVCLDNCTYYILPNIFTPNGDGINDYYTP
                     VKPYYFVEEIRMTIQNRWGIVVFETTDPEIRWDGRYFKNNRIVSDGVYFYVCDVYEQR
                     LTGLNIRHLAGFIHVITATDVNSTSE"
     gene            21251..22519
                     /locus_tag="PLK75_05565"
     CDS             21251..22519
                     /locus_tag="PLK75_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY38750.1"
                     /translation="MTKISILIPILVCCWFSVQSQSFDYSITFSASGETSLVETVTVQ
                     NMTQCTEETLSGSSTLHLVYSSSIGEIDQNNGFFVYPNPTTDQCIISFPVFESGLVQV
                     KLSEISGKVLYNDEFQMQAGNNKMIVTGLGFGILLLQIKTTQTTFTKKIIGSADENVA
                     PSVNVEFLENSGIIKSSQTKEIVDMDYNPGDILLFKGVSGNYSNFITLVATESTLVTF
                     HFNDATDGDNNHYATVVIGEQTWMAENLRTTTFIDGSPIPKMEDPTEWAQASGPAFCW
                     QENDSLTYAESFGALYNWFTVSNSGKDAMSICPLGWHVSTEDDWVTLGEFLGGNPIAG
                     GKLKTFCGEFWSEPNEGATNISGASIKGTGLRTISGVFGDTMYQSYFWTPKQYLPENA
                     RFINVSYITSELNFYDNVLTYGFSVRCVKD"
BASE COUNT         7184 a         4576 c         4634 g         6510 t
ORIGIN      
        1 atgaaacctg attaaaagga tatcgcagta catacaatag ttcatgggaa ttcgacttta
       61 taatgttata cccattactg ctatttattg tcatgcgatg aaaaatgtat tgtttgtcga
      121 gccttttctt taaatcttcg aaactaagga gatattcatt actgtcgaaa tttgcagaaa
      181 accgcacgcc cccggtaatg cgataatctt caaaaaggtc tttggccccg agtttgaaga
      241 gaatattaaa gccagggtta aaataaaatg cacttcctgt gaattgttga taagaagaat
      301 ttagaaaacc aaagtcaatt tggtttacaa catagttcgt ataaaatgta gttacataga
      361 tttgctgaaa ctttctaagc ggaactccca aactatcgaa atcaccactc tctttcctgt
      421 ttgtttgtaa cacctcctgc gaaaacgaat agttattgat atcaatattt tcgagttcat
      481 tgtgctgtat tggcttaacc ggcttttcag gcttgggttg gggtataaaa tttaggtttt
      541 cacggtctac tgtccggaat cgggtgtcag taagatttcc tgtaaatggt gtgcgagata
      601 tttcggaaaa gtaaataccc catctgtcgt tttcgaaaac actctcaacc accgaggggt
      661 atcttgcagc tacgtcatag ctgccaatat ttcgatggta attggtaacc ggaaacgaac
      721 gggtaatgta acgataatgg gtagctgtgt cgatgtagct gattacactg tcgtatcgtg
      781 catagtagcg gttataaatg ccattatcat tagcaagata ccaaaattcg ttcgttttaa
      841 tactcagtgg tttagcctca ttgatatcct ttgtttttgt aacccgcttc aaaacattgt
      901 ttttaccgtc ggcatccata ataaaaatat cggagcagat ttgcattggt acaacattgg
      961 ttttacctga tactaaagtg tcggaagcgc gattgctgga aaacaatatt tttgaagact
     1021 tctcaatgaa ttgaggggaa tagtcatccg caatgtcatc agtcatattg gtgatagtgt
     1081 ttgccacaat attgaaaatg tagatatcac tcataccatt cttatagcca ctcattacga
     1141 gtttcaaacc atcatgggag taagaaaatg aaaggacctt ctgtaaaagg gctatctctc
     1201 tgtataaggt cttcttggtt tcaagattgt aggtacaata gaagacctct ccattacttt
     1261 cgatgatgta tccgagcaat tttcccgtgg ggtgccagct aataactgga taactgtagt
     1321 cgacaatctg ttccagttta tgttcttttg ttaggattgt tttggtttta ccacttgctg
     1381 tttcgtagag tacaatttta tatttcccca tattttttat cgaatacgca atgtaattac
     1441 cattgtcgct gatttctgtc tgaatcagtt cccatttgcc aaaacgaggt tttaaaatat
     1501 tttttttggc attaaaatcg cagcttaaat cgtgttcggc atataaagcc tgatagtaat
     1561 tcaaccaatc ataggcaagc attttcagat tattccccag tacgtactga aaaccatttt
     1621 caacattttt tgttactctg gtcaggtaaa cgatagttgg aattacgttc tcaccatagt
     1681 tatttgcaac aaagtaccat attgaatgcc ctgcaaactg agcgtctttt cccgaaaggt
     1741 gattgaattg ctcgaacttg cccgataata ttccgtcttt cacataatta tctatttcat
     1801 aatcccattt attcgaaata taggatacaa gccctgaaaa aaaccagtca ggaatcgaga
     1861 tgagagtgtt gttagcaatt tttgaggtaa aacccgatcc atagagcatt tcattgagca
     1921 ttacttctgc aatagcccct ctaatctgaa tttccatttc ccggctatcg ccttcggcat
     1981 ataaaaacac aatattatct acaatgcgtg ttactccccc aatattatac tgagagtctc
     2041 ctgtattaag cccaatgttg ctctgcctga aatcggaaag gttgttataa accacaaaaa
     2101 tcaagcgttg ttcaatttta taatccaaaa atttttcaag ttcgggtatt actttatttg
     2161 caatttgagc aacctgtatg gcatttgctt gaccaccgct ataaaaatat gtatcgaacg
     2221 ggttgtacct gtaaaaattc caatcgaaat tcccgtactg cacgcggttt tttccaaaat
     2281 tcatctgatg ccctgaatag aactgtggca atgcattcga ccagcaaatc aacattgaga
     2341 gcagcaaaaa aactatctta taaaaacgca gcattctatg agctcataat tttttaactt
     2401 tgcaaattag caatttatta cgcacaactc aaaattaatc gatggctgtt ttaaagatta
     2461 tctcaggcgg gcaaacaggt gccgacaggg cagctctcga ttttgctatg agtcataacc
     2521 tggattgtgg agggtattgc cctaaaggtc gctgtgccga agatggtgta attgatctga
     2581 aatacccgct taccgaatca ccgtcctcgt tttatcagga gcgtacaagg ttgaatatca
     2641 ttcattccga ggccacggcc ttattcatag ttgatggagt attcgggaag ggcagtttat
     2701 ttactaaaaa acaatgtctt accaatagaa aacctttgct gattgtagat ttaaccgaca
     2761 acgcagcaaa ccttgttgca ggttttacaa agtggatttc ggagaataaa atcagtatat
     2821 tgaacatcgc tggtaatcgt gaaggcacat cccccggaat ataccgtctt acaactgaat
     2881 tcttgatgct tgttcatcaa aataccgaag tattttgcaa tgaaaaaacc tcttgaaata
     2941 tcaattttgt tttttttgat tactatcatg gaattgatgg ggcagaattc attgttctcc
     3001 caacaggttc cgctgcctta cgagcttaga ccagctcgtg aagccatgtt cctaagcata
     3061 gggattcctc tgactgtagt accgttttac gttaagtcca caaccaaatt tcttgaccaa
     3121 tcggagattg aacaactaaa tgccgacgat gtaaacattt ttgatcgttg ggttatcgat
     3181 aattggtcgt taccattaag gaatacttcc agaatgttac cctatgtttc gggcggtatt
     3241 ggagcaacaa ccctatttat tgtacctgcc ttcaccgacc aatcgcgccc atacctttcg
     3301 tatgtaggaa cgctttcgct gatgtatatt gaaggggtgt taattagcca gggtttgtcg
     3361 ttcatgatga atacttacat caatcggccc aaaccctacg tttacaacac caaactgccc
     3421 atggagtttc gcgtttttca gggaaataat gcttcgtttt attcgaaaag ggcttgcttt
     3481 actttttata acgctctttt ttcaaccatg gttatgtacg atatgtttcc tgataaaccg
     3541 gtgtggttat atgtaggtgc aggcttattt ggtgtagccg ggctcaccgt tgcagcagag
     3601 ctcagttccg gagagcattt ctacaccgat gtgctgacag ggatggcaat aggtactgcc
     3661 acggcttatg ctatagtaaa atatcatcgc gtcaccaacc ttaatcaacg attaacaata
     3721 ataccagcaa ttggccccaa aacagcgggt atttatctga attacaggtt ttattaacat
     3781 ggcttgtgta tacattcgca ttcaagcgta ctcccaatat ataaaattca ttaaatttgt
     3841 gttttaaata ttattatata acatggcatt tcttagcttt ttaacaaaaa tgttcggtac
     3901 caaagcggaa agagatttac gcgaaaccca gcctctagtt gattctattc tttcggttga
     3961 accagaaata gcaaagctat caaacgatga actcagggcg ctgactttta aaatccgtgc
     4021 tgaaataaga gaacacatac aagatgaaca gaatcagata aatgaactga agcaaaaaat
     4081 agaaaccctc gaaataaacg aaagggaagc tctttttgac gaaattgata aaattgaaga
     4141 tgtaatcacc gcaaaaattg aagagattct gctggaaaaa atcccagaag ctttctgcat
     4201 tgttaaagaa acagcgcgca ggtttacgga aaatgaacac gtgagagcaa cagctctcga
     4261 ttacgacagg gatttggctg ccactgttga ttttgtcaac attgaaggag atcaggctgt
     4321 gtggagcaat tcatggatag ccggaggaaa tcttacgaaa tgggaaatga ttcactacga
     4381 tgttcagctc ttcggtggtg ttgtattgca taaagggaat atcgctgaaa tggctaccgg
     4441 agaagggaaa actctggttg caacattgcc tgttttttta aatgcactac caggcagagg
     4501 cgttcacttg gttaccgtga acgactatct tgcaaaacgc gactcggaat ggatgggtcc
     4561 gctatttcag tttcacggac ttacggtgga ttgtatcgat aaacatgaac cgaacagtga
     4621 agcaaggcgg aaagcttact tgtgcgatat tacgtacgga accaataatg aattcggatt
     4681 cgattatctc agagataata tggcgatttc gctgagtgat cttgttcagc gcgggcacaa
     4741 ctacgctatt gtcgacgagg tggactctgt gctcattgac gatgcccgta ccccattgat
     4801 tatttcggga cctacacccc ggggcaacga ccagcttttc gacaatttca aacctcaggt
     4861 aatgaagttg tattcggctc agcgcgacct tgtgaatcaa attctggtgg aggcaaaaaa
     4921 acttattaag gataacgaac gcgataaagc cggatttaaa ttactccagg catacaaagg
     4981 cctgcccaaa aataaagcac ttataaaatt cctgagcgag gaaggcatga aagttctgat
     5041 gcaaaagaca gagaattact acatgcagga caactcaaaa cagatgcata aagttaccga
     5101 tgatctgtac ttcattattg atgagaaaca aaatactatt gagctgactg ataaaggtat
     5161 cgacctcctt tcgcaaagca acgatgattc gagttttttt atattgcctg atattgggtc
     5221 ggaagtagcc aatattgaaa aattggggct aaacgacgaa caaaagctgg aaaagaaaga
     5281 tgcgcttata agagattata caattaaatc ggaacgtgtt cacactgtaa accagctact
     5341 caaagcgtat acactgtttg aaaaagacac cgaatatgtt gtaatcgaca ataaggtaaa
     5401 aatagttgat gaacaaaccg gccgtatcat ggaagggaga cgctactccg acggtttgca
     5461 tcaggctata gaagcaaagg aaaacgtaaa agttgaagca gcaacccaaa cctacgccac
     5521 aatcacgctg cagaactatt tcagaatgta taaaaagctg gccgggatga ccggtacagc
     5581 cgaaactgaa gcaggtgagt tttgggatat ttacaaactt gatgttgtgg taattcccac
     5641 caacaaacct atatcccgcc acgatgacga agatatggtt ttcaagacca agcgtgagaa
     5701 gtacaatgcc gtgataaacg aaattgttga tctcacagaa gcagggcgtc cagtgcttgt
     5761 tggtactacc tctgttgaaa tttcggaatt actatctcgg atgcttaaat taaaaggcat
     5821 caaccacaat gtgttgaacg caaaacttca taaaaaagaa gcagatattg ttgccgaagc
     5881 tggtagaagt aaaaccgtaa ccattgccac caacatggct ggtcgtggta ccgatattaa
     5941 actcagtaaa gaagtgaaag aagccggagg tctggcaatc ataggcacag agcggcatga
     6001 atcaaggcgc gtcgacaggc agttgcgtgg tagatcaggc cgacagggcg acccgggttc
     6061 atcacaattt tttgtttcgc tcgaagatga cttgatgcgc ctgttcgggt cggaaagaat
     6121 tgcaggactc atggacagaa tgggactgaa agaaggtgaa gtaattcaac attccatgat
     6181 ttcaaaatcc atcgaaaggg ctcagaaaaa agtggaggag aacaactttg gtattcgtaa
     6241 aagactactt gaatacgatg atgtgatgaa tgcccagcgt acagttatct ataaaaaacg
     6301 caagaacgct ctcgatggag aacgccttgg cgttgatatt gccaacatga tgtatgacat
     6361 ctgctccgga tttaccgaag agttttacgg cagcagcgat tacgaagcat tcaatttcga
     6421 aatgattaag gtgttctcta ttgaggctcc tgttacgaaa gcagaattct ctaaaataag
     6481 ccatgatgat ttgactgaaa ttgtttttca gaaagtaata gaaacccata agagaaaaac
     6541 aaagacaata gcagaacaag cgtatccggt aataaaacat gttttcgaaa atcagggttc
     6601 aatgtataca aatatcattg ttcctgtgac ggatggcata aacacctaca aagtaattgt
     6661 aaatcttgaa aaagcataca actccgaagg gaaagaaata tgttcggcat acgaaaagag
     6721 tttggtctta agtattatag atgatgcatg gaaagaacat ctccgcgaaa tggacgattt
     6781 gcgacagtcg gtgcaatcgg catcatacga gcaaaaagac cccttgctta tatacaaact
     6841 cgaatcgttc aatcttttca aaacactcat agaaaaagta aatcgctcca ttctcagcac
     6901 tttaatgaaa gggtatattc caataagtga tccggggcag gtttcggaag caagacagca
     6961 acggcgcctc gacacaagca ggatgacagc aggcagatcg gaacaggcct acaacgatcc
     7021 aagcaaggga aaacccagca atcagcctgt gaaagtagaa aagaaagcag ggagaaacga
     7081 cccctgtccg tgcggaagtg gaaaaaagta caaacattgc cacggggcag gggtgtgatt
     7141 tattaggtat aattattgta aaccaacatc tgaattaggt ttcaacatga taaaacagac
     7201 caaaataatg aaaaagctaa gcctactctt actggttatc tcaggttcaa tgtattgtta
     7261 ttctcagaac aatattaatg attccattgc aacctatatc gatacaacac aaattataat
     7321 accactgaaa attggtatcg attcggtagc cgcaatttcg gataccagct caacactggt
     7381 tcttgccgaa aaccaagaag cagagcttgc ttacaacgat ggggtaatgc tatttcagaa
     7441 tggtgactat aatactgcca tttacaaatt tacagaagcg ataaaacttc gcaataattt
     7501 cgataaagcc tacttcaaca gagcaagtgc acatatgcaa cttcaggagt tcgatctggc
     7561 ttacaacgat tttgattcct gcttgatact caacccggaa aaaatatcag ctctctacta
     7621 catgggttat attagctttt accgacaaga ttattctaaa gccgccggat ttttcgatat
     7681 ggcaattgct aaaggattca gcgatgcaaa catgtactat ctgaaaggaa tcatgagttt
     7741 caacaataaa agctttgaag aggctattac caatttctct tcaagtataa aaattgacgg
     7801 caacaatttt atgccatatc acgacagggg ctctgtttac agactaacgg ataaaaacga
     7861 gcaggcaata agcgactatt caaaagccat agaactaaac ccgaaatgct atcaggcatt
     7921 caacaacagg gggtcggttt accgcatgat gaaagattac gacaaggcga ttgccgatta
     7981 cgataaggcc attcagctga aagaggatta ctatattgcc ataaataacc gcggtgtagc
     8041 gaaatacgaa accggtaaat atgaagaggc tatcgacgat ttcgatgaag tactgcgtat
     8101 taagaaatca tacgcatttg cttacaacaa caaaggtctt gcccaatata gactaaaaca
     8161 atatgctcct gcagtggaat cttacaccaa agccatacaa tacgatccaa attatggttt
     8221 tgcatacctg caccgcggga gtgctcagga aatgctgcgc aagtttgatg aagcttgcgc
     8281 cgactggaaa aaagccggtg aacttggtgt ggatgctgca aaaaattatg tcggaatttg
     8341 taatgaataa cactattacg actatgaaat atagaatatg ttttatcgga gttttgttag
     8401 caatatcttc gtatctgacg ggtcaggaga aagttgagat actgaaggat gagtttaaaa
     8461 ccccaaagga aggattcaaa gatgcctggg atgcaataaa agagggcgat aaattattcg
     8521 acttcggaga agccggtttc cggaatgcat tataccccta tcttgctgca taccagtaca
     8581 atcagaaaaa cgcagagctg aattataaaa tcggcatttg ctacctgtat tctgttgaaa
     8641 agacaaaggc aataacacac tttgttgatg cggtaaagct taaacccaat gttgcgggtg
     8701 atattgatta ttttctgggc cgggcttatc atctgactta tcaattcgat acagccatgt
     8761 attatttcca gagttataag cgtaaagcag cagctaaagg tgttaatatt acagataaag
     8821 atgttgacaa acgtattgat gaatgcaaat atgccaaaca gtatgttgca agccccatta
     8881 gggtttttat cgacaacctc ggcgacaata taaattcctc ttaccccgat tatgctcccg
     8941 taattaccgc cgatgaatcg gttattgtat attgttcacg gcgacccggt ggtgttggcg
     9001 acaatatcga ccctaacgat ctgcaatact acgaagacct ctacacatca gaaaacatta
     9061 aaggcagggg gtggagccct gcaatcaaca tgtcaaatct gaatactgag tatcacgatg
     9121 cctcagtagg catttcgccc gacggacaaa ttatgttcac atacaagggt gttcccgacg
     9181 ggaccatttt ccaaagtgag ctaaaaggcc tctcatgggg aaaaccaaaa gagttaccct
     9241 cgcaaataaa cacaaaatac gatgaatcag atgccagtct cacttacgac atgaaatatc
     9301 tttattttgt aagtaatcga ccagaagacg ggtttaaaac accaactttt ggcggaaaag
     9361 atatttttat aaccaatgtt gacgcaaaag ggaactggct tccctgtaaa aatatcggag
     9421 gaataataaa tacaaaatac gatgaaaggg gagttttcct tcaccctgat gggaatacca
     9481 tgtatttcag ttctcagggt catgaatcaa tgggtggatt tgacattttt tattccgaaa
     9541 aggacagcct tggcaaatgg ggcaaaccag ttaatattgg gtaccctgta aacactcccg
     9601 atgatgatgt ttttttcgta gctgccggga atgcacgatt tgggtactat agtagttcgc
     9661 gcgaaggcgg ccgggggtat caggatatat accgtatcac tttccttggt cctgaaaaac
     9721 tcatggtgtg cgggtcggaa gacaatttac ttgccggaag tgagatggcc gtacaacaga
     9781 aagttgatgt acagcaaacg gttgaagttc gaaaagtgcg tttgacaatt ttaaaaggca
     9841 ccattaccga ctatctttcg ggagaacctg ttgaagccga gatcgaaatt gttgacaatg
     9901 aaaaaaatga agtaatttct gtcattaagt caaacagctc aacaggtaaa tatctggtga
     9961 gcctgccctc gggaaagaat tatggcattg ctgtgaaagc ccccgattat cttttctaca
    10021 gtgaaaactt cgacatacct tattcgcaag attatcagga agtaaccaaa gacattgtac
    10081 ttagtaaggt gaccgtcgga tctaaaatca tcctgaaaaa cattttcttc gattacgaca
    10141 aagccaccat ccgcccagaa tcggaaaacg aattaacacg ccttgttaca ttactgaatg
    10201 catatcccaa aatgcgcatc gaaataggag ggcataccga tagccacgga agtcttcaat
    10261 acaacacaaa gctttcaagc gaccgcgcca aagccgtggt tgattacctt gtaagtaagg
    10321 gcattgatgc atcacgactc gagtacaaag gatatgcttt taatgagccc attgccacca
    10381 acgataccga tgaagggagg caacaaaacc gccgcgtaga atttaaagtt ctgagtatta
    10441 aataaacagc catgaacaaa actataattt ccttactgat agcagttttc gttaacacag
    10501 cacttaatgc acaaaccgat gttaagttca aaagctcaaa cttttcgaac aatcccaaag
    10561 gatttcagaa cgctgttaat aatattaatt cgggagatat aattattgaa gaaaaacgta
    10621 attaccgtga agccctgaaa tactatctcc aagctcagga ttttaaccca gattgtgctg
    10681 aattgaacta taaaatagga gtatgttact accattccga aaaaaaacat atgtgtttgc
    10741 cgtacttcga aaaagcttat ctgctcgatc aaaaagtaaa taaagatatt tgctattatt
    10801 taggccgcgc atatcaccta aactatcagt ttgaaaaagc actgactttt ttcgatatgt
    10861 ttaaaaattc aaggggtgtt acttccgatt tgcttaagct aacgcccaga catattgaag
    10921 attgcaacaa cgccatgaca ctcattctcg acacattaca ggtagagatt gtaaatttag
    10981 gaccctccgt aaacacccaa taccctgaat acaacccact tatcactgcc gatggtgaag
    11041 agatgttctt catcagtcga agagaaggta caaccggtaa taaaaccgat atcaatgatg
    11101 ggatgtattg cgaagatgta tatatttccg atctggtaaa tatcaactgg acaaacgcta
    11161 aaaaccttgg aaagcctatt aatactgcga agcatgatgc tgttctgggt attgcgccag
    11221 acggccagca attgctgaca tatatcgata ttaatgaggg agatattgga atttccgttt
    11281 tacatggttc aaaatggtcg aagcccgaac tccttaacac gaatatcaat acgcaatacc
    11341 acgaatcgaa agcctgcctc tcaccggatg ggaagcaact tttctttatt agtaacaacc
    11401 ccaaaaacag cataggtgga agagatattt ttgtgagcaa acttgggaaa aacggagatt
    11461 ggggtcctgc cgaaaatctg ggcaagacaa ttaacaccaa attcaacgaa gaaggggttt
    11521 ttattcatcc cgatggtcgt accatgtact tcagttccga aggccataac tcaatggggg
    11581 gttacgacat cttcaagtcg gtgcgcgata gcttaggcaa ttggtcgaaa ccggtgaata
    11641 tcggattccc tattaacaca cccggtgatg atgtttttta tacagtatcg gcaagtggca
    11701 aaaaagcata ttttgcttcg taccgcgaag atgggtacgg tatgtacgat atatatgaaa
    11761 ttcgattcaa agagaagaaa cccgatgcaa ttgcgacaaa tgaacctgaa aaagtattgg
    11821 ttaccgtgct gaaaggtact gtaaaagact cgaaaacatt aataccaatc gaagccaaaa
    11881 tcgagatcat cgataatgtt aaaaacgaag ttgttgctac tttctattcg aacagtgcca
    11941 cagggaagta ccttgtaaca ttgccttccg gccgtaacta tggaataaat gtaagccacg
    12001 attcatacct tttccattca gaaaatttcg atattgccga cactaccgat tatcaggaga
    12061 tagtgaaaga tattttgctt caaaagattg aagtgggaac aaaagtggtg ttacggaata
    12121 ttttcttcga ctacgacaag gcaacacttc gcccggagtc agccgcagag ctcgacaaac
    12181 tgattgccat tctcatgaaa gagcccatgt tgagaatcga aatatcaggg cataccgaca
    12241 acaaaggcag tcatgagtac aataaaaaac tttctgaagg ccgggcaaaa actgttgttg
    12301 actatcttgt agagcatggc atcaaacgcg ataggcttga gtatgctgga tatgcattcg
    12361 atcagcctat ctccgataac gacaccgaag aagggcgcca gctaaaccgt cgtgtcgaat
    12421 tcaaagtact gagccgctaa taccgaatgt ttgttaaatt ttgttaaact gaaagtccgt
    12481 taattgttga tggctaaaaa atgtgtattt tgccaaaaat cattgaatgc gcttcctgag
    12541 aattatatca gaaaacttta aaattgcatt gatatcaatg cgatccaacg tattacgtac
    12601 aattcttaca gtccttatca ttgccgtggg aattatggct ctggttggca ttcttaccgc
    12661 tgtcgatgcc attaagaaca ccattagcag cgaatttacc aatatggggg ccagcacatt
    12721 taccatagta aaaaaaagga tgcacacccg tggcggcgaa aacgaactca ggcaaactgc
    12781 catccgtgat ataacttacg atgaagcaag gcgctttaaa aattcattct cttttcctgc
    12841 cagggtttcg gtaatagttg atgctacctg gacagcgact gtgaaatacg aaaatgaaaa
    12901 aacaaatcca aatattcagg taatgggttc cgacgaagat tacctgttta catccgggaa
    12961 tgagattgga ttgggcagaa atttttcacc caacgaagtt caaagcggaa gtaatgtcgt
    13021 aattatcggg aatgctattg cggaagacat ttttaaaaac aagcagtcac cgattgataa
    13081 atatattaac attggtaacg gcagatacct tgttatcggt gtcttgaaat cgaaaggtac
    13141 cagttttggt ggagctggcg ataaattttg catcatacct cttacaaatg cccggcaaaa
    13201 ctttaccgac tacgatcttt ctttcgatat cagtgtgctg ccatacgatg ccggcaaact
    13261 cgaccttgct gtaagtgaag ctgaaggtct tttccgcaat attcgaaagc tcacagtgta
    13321 cgatgaaaac gactttgaaa ttcgtaaaag cgacagcctt gcaaatatgc ttatcgaaaa
    13381 tatatcagtg gttacaattg gtgcaaccat aattggactt attactcttt tgggggctac
    13441 tattggactg atgaatatta tgctggtttc ggttactgag agaacacggg aaataggaat
    13501 cagaaaagcc ttgggagccc catcacgtgt gataaaacaa caatttctct tcgaatcggt
    13561 agtaattggt caggttggag gcattctggg tattgtcctg ggtattgttg caggaaatct
    13621 tgtatcaaca gcaacgggcg gaagtttcat tatcccatgg atgtggattc tgcttggggt
    13681 attactttgt tttattgtgg gtgtgatttc cggatatttc cctgcagtaa aagcagcaaa
    13741 gcttgatcct attgtggcac tacaccatga ataattgata acctgaattc gttcttcgta
    13801 aatcttatca attcatctcc ggttagcaaa ttggcaccta ttgaacacaa ttaaatccta
    13861 cagaacccgt atatgagaaa actccgaatt gaagaactaa acagaataaa tgttgagcag
    13921 tttaaaaaca ttgctaaaat tcctttggtt attgtactcg ataatatccg tagtcatcat
    13981 aatacaggtg cagtttttcg tacggccgat gcttttctta tcgaaagact tcttttatgt
    14041 ggtattacgg gctcacctcc ccatcgcgat atttacaaaa ccgctttggg tgcctgcgaa
    14101 tcggtggaat ggaagtacta tgaaagcact ttggtagcgg taaaagagct gaaacaaaat
    14161 aattatgtgc tgctggcatt ggaacaagct gaagggagta ttcccctcag cagctttcag
    14221 gcataccccc acttaaaata tgcgctgata atagggcatg aagtaagagg ggtacaacag
    14281 gaagttctgg atttatgcga catgtgcctc gaaatccccc aacacggcac aaaacactcg
    14341 ctgaatgtta gtgtaagtac cagtatagca atatgggaac taacaaataa attaagcaag
    14401 gcatgagcga aacctgggat aaccggtata gtacaagcac atttgtttat ggtactgaac
    14461 ccaatgtttt ctttaaagag gtggtactgc aaaatactcc cggaaaaatt ttacttcccg
    14521 ccgatggcga agggcgaaac agtgtttatg ctgcaagcat tggatatgac gtccacgctt
    14581 tcgatttcag tttagtggct atcgaaaagg caaggcaact tgctactgca agaaatgtta
    14641 cagtcaaata caaacatgct gattttaatg ttgcagataa attgtacccc gacaatagct
    14701 ttgactatct ggcacttatt tatgcacatt tccctccgga aattcgcaaa ttgcaccacc
    14761 acatgttact tcgcaaactc aaaccaggag gtaagcttat atttgaagcg ttttcgaaaa
    14821 aacaaatgcc ccgcacttca ggagggccta aaaacgaaga gcttttgtat ttaaccgatg
    14881 acctaaaaca tgatttcggc tcactttcga agctgtcggt taccgaaaac gaaatttctc
    14941 tcaacgaagg tgagcttcat aagggtgctg ctgatgtaat tcggattcaa ggtgtgaaat
    15001 aatgcagcat atttgaaaaa aaagttgtct acgcagcata aaatttcaac aggtaacatg
    15061 aaagtaatta cttaattttg cagccatgtt tatcgaaggt ttaacagcat gggggctcga
    15121 tgcttggatt attatactga tccttgttgt ttcggggttt gtggtgggag taatcaatac
    15181 ggtagccgga agcggaaccg taatctccta ctcattattt atggctctgg gtatgccctc
    15241 ccctcttgcc aacggtacag tgcgtttaag cgtggttatg caaacgctca catcgtcgct
    15301 tagtttttac agaaataaaa aacttgacat aaaaaaggga ttgatccttg ggatcccaat
    15361 cgtaatcggt tcaattgccg gggcacagat tgctgcaagt atcaataagg atctttttga
    15421 aaaaattgtc ggaatttcgc tattaatact tttattcttc atcttctata acccagaaaa
    15481 gtgggtaaaa ggctctctcg aaaaacaaca aagaaaagtt tctgcctggc agtatttggt
    15541 ttatttattc ataggcattt atggtgggtt tcttcatatc ggagttggca tttttctttt
    15601 gtcggctttg gttcttcttt ccgggtacga tttggtaaga gcgaatgctc taaaagtatt
    15661 tcttgttttt ctttattcac cggttgcttt agcaatattt attatgaacg ggcaggtcga
    15721 attcctatta gggtctattg ttgcggtagg taatattttc ggtgggtggg ttggatctcg
    15781 aattgctatt aaaagaggtt ctggctttat cagaatactt cttgttataa ttattgcaat
    15841 cttcagcgcc tatttgctgg ggttgtggga cttggtattt taatgaaaaa acaggtatgc
    15901 gactacaata gctgctatta taccagcaaa atcggcaatc agtccgcaag ttaaagcata
    15961 gcgggttttt ctgatactaa cggacccaaa atatacggca agaatgtaaa acgtagtgtc
    16021 ggtggccccc tgaaatacgc aagataaacg tcctacaaaa gaatcggctc catactgatt
    16081 cattgcatcc accattaaac ctctggcacc actaccactc aagggtttca ttaaagcaac
    16141 gggtaatgag ggaacaaaat cactgtttac tcccatagct tcgaaaaccc atgctattcc
    16201 atatattata aaatccatcg ctcctgatgc cctgaaaaca cctataccaa caagtatggc
    16261 tatgaggtat ggaatgattt taattgcagt actgaaccct tctttagctc cttcgataaa
    16321 agaatcgtag acattcactt tttttcgcaa agccaatgca atgaagctga tgattattgt
    16381 gaataaaatt aagttacctg caatcgatga taccgtatta atattctctg atgataaggt
    16441 agagaaatag aagattattg cggctataaa caccgataag catcccaaat acaataatat
    16501 ggttttactg aacaggttaa tcttctgcac taccgataca gcaatcaaac ccgccaatgt
    16561 acttataaaa gtagttatca aaataggaat aaatatatcc gacgggtcag cggctcccat
    16621 ctgtgcacgg tataccatta tgctgatagg gattattgtc agccccgaag tattcaaaac
    16681 aagaaacatt atctgtgcat tcgatgctgt atctttcctt gggttaagct cctgcagctc
    16741 tgccatggct ttcagtccca gcggtgttgc agcattatcc agccccagca tattggcagc
    16801 cacattcata accattgatc cgtgcaccgg atggttttta ggtatttcgg gaaatatctt
    16861 gttaaaaaac gggccgataa gcctcgccaa aattctgatt gcaccgccgt tctcccctat
    16921 cttcataatt cccagccata aagtcattac accggttaaa ccaagtgata gctcaaatcc
    16981 cagtttggcc atatcgaaag tgctgttcat aattgaggta aaaactaccg tatctccaaa
    17041 aaacagaagt tttaccaatg caacaacaaa agcaataata aaaaaagcaa tccagatgta
    17101 attcagtacc ataccatgaa ttatctacca taaaaataga gctttgtttt agctgacaaa
    17161 gaatacacga tgaaatagta tcaacaaact aattaacagc agctgtcaaa ttatttacaa
    17221 tactgttgta ataagaattc cgcttcgggg tgacccaaat gcgccgcctt gtgccagtag
    17281 tagcatgctt ttgcgttatc atcaagccgg tgtttgcacc atcccagcga aatgtatgtg
    17341 tctgcattcc ttgggttcag gtcaagagcc ataaggtagt cgcttaccgc accggtatag
    17401 tttgtcaaat ttgaataggt ctctgcgcgt aataaaaaat acaactcatt accggaattc
    17461 atggcgataa gttttgacaa cacatctacg gcttcggaat tgattccggc attcaataaa
    17521 acaacagaaa gtttatacca tacctcttca tgatcttcga agaagcgcag ataatacctc
    17581 aaatcgctga ttgcctgttc ataaatctga gcattattac tggctaccgc acgttgtaaa
    17641 taataatcta tttcgtatgg gttcaattca ataacgtcag aaatatcgat aactgccttg
    17701 atatattcat tcatagctga aaatacttct gccctgcgca acaataactc atatgacagt
    17761 gtatctttta aaatcccgtt tacctttttc agtgcttctt tatattgctt cagttcaatg
    17821 agggcgtctg tcttcatcaa ctctgccatg taagtataac gcgaattgga gaaactgtta
    17881 ttatcatgca gtacttcagt aaattccttc tgtttcaaca aatattcaag ttctgcaagt
    17941 accagtttct ctgaatcagg ttcaaaaccc ctccaaaatt cactccattc cttcgatgca
    18001 aaaatgtacc tgaaacatgt atccgcttta acatctgcaa tattaaaaac ttctggaagt
    18061 gtcaggaact ccttcagctc attaatcgct gcctcaacat cattgacaag tgcaaggcaa
    18121 cgggccatat tcagacgggc cgtcgcctga tcatacggta ttagcaatgc atacatacgt
    18181 tgagatttca aatagtttcc ggttttaaaa tacagtccgg cattcagtct gataaataca
    18241 gcatcttccg ggaaaatacc gacaagagag tcggacaatg caagagcagc agatgtatgc
    18301 cccttttcaa aatccgataa aaccctgaaa taataatctg ctttgctttg accaaaggat
    18361 gcattcaaac ttatcaataa caatattgta gaaattatca gcttcatcat tcagaggtgg
    18421 agttcacatc cgtggccgtt atcacatgaa taaagcctgc aagatgcctg atattcaatc
    18481 ctgtaagtct ctgttcatat acatcgcaaa cgtaaaaata cacgccatcg cttactatcc
    18541 ggttgttttt aaaatatcgg ccatcccagc gaatttcagg gtcggtagtt tcgaaaacga
    18601 caattcccca tcggttctgg attgtcattc ttatttcttc aacaaaataa tagggtttta
    18661 ccggagtata atagtcatta attccatctc cattcggcgt aaaaatattg ggtaaaatat
    18721 aataagtgca gttgtcgagg catactctgt tcgagggtaa acttttattt ccaaaagaat
    18781 cgacagcaac cactgtatag caccctgcca tggtttcatc gggaaaatgt cggaagaatg
    18841 ccgtatcatg atttgcactg gaatagacct tattctcaat accatcaaga actggagaat
    18901 aatacacttc gaagtaagca acatcaatca ggcagctatc tggtatgaac cagctaagcc
    18961 ggttaaacag gctgtcgcaa acactcactg cagtaagaac gggaggacac ggaggcaccg
    19021 tatcgatagg cgtaccgcaa ttttcctgcg acaggttaat aattgggtta acgaatcccg
    19081 gtatgccata cgaccctttg ctgcgaacaa aataacagta ttgtgtgcca ttatcgagtt
    19141 gtgtatcgcg gtaataatca ttcccggtaa tatcaagtga atcgaaaagc atggtttctg
    19201 gactttgttt gtacacggtg tattctgtat taacccatgg aatattttta tcgaaatgca
    19261 ataccaaagc attgtcgtcc gattcgatgc gcaagaaaac agaactggca atatgtggtg
    19321 ttcctatgag aaagcggtta ccttcctcat cattgtagaa ctctatcctg tatgagtacg
    19381 ggttttccaa agtattaagc aaactgtcat aataaatagt atcatttatc ccagccagcg
    19441 aatcaatacg ttcaaggttt tcaccaaaga gaccttccga cctgtatatc aaatacttat
    19501 aaggacctgg agctgcaatg gcatcaaatt ctgtaggttt tgaccatgcc agatatattc
    19561 tgccttcatc aacgctggtt tccagtacac taacatttgt aattgtggga atacctctaa
    19621 ccaactgtgt acaaacttct tcggaggcat agccctcgat tccgtcagga aaaacgggtg
    19681 taataatata acagtacaca aatccctgcg aaagtcctac cccgtcatta tcatcaatat
    19741 aagttgttgt agtatgtcct gcaactgttt ttaccagggt ataacctgtt tcttcgggaa
    19801 ctcccacttc gcaggagtct ggtacaaatc ctgagggatt gattctcctg tacacattat
    19861 acccggttac attgtaacat gcatccggag tccagctaag tgtaataaaa tcgttacctg
    19921 acgaaatgtt cacattttcc actgccggtc caacgacata tatgctgatg tttttaatgt
    19981 acaccaaatg cacttcgctg ttatcatcct cagctttaat atttacgata tagggctgcc
    20041 tacgtacatg ttcacacaca gtctgccatg taaacaaacc ggttgctgtt ccatttccgg
    20101 tatattccga ttcaattatt gcagcacctt ctgtcatgat aaaagggccc ccattggctg
    20161 taattgacaa agaatcgcca tcatcatcag tcactgtgaa aggcatttct agtttggttc
    20221 ctgccagtat acaaataact tcctgagcat taatttgcgg tgcatgatta tcagtgtcat
    20281 atacctctat ttgcatatca cgaatgattt ccccaatctt aattccttta cgccattctt
    20341 caataatcat ggcaatatta tacacacccg tatacattgg tgtattccat acaagatcac
    20401 cggtaagctc attcatcgtc agcgagttac tggcttcggg gaaggtatac ccttcgattg
    20461 gctcaccatt ttcgccacga cagattgtga gcttataggc caggctatcg ccatcagggt
    20521 cgaatgcccc tggattgtga ataaatagct gcccgactgc tgccttatcg acaggaggct
    20581 gagtgagaac tggtgtactg tttgaaccca aagctgcatt gatttgcata gtggttgaaa
    20641 ttgcaaatag tgtattcact gaattgggta tgttttcaac gccaaaattt ctgtttgggt
    20701 cttctaccac taaagtatat attccggggc ccggatatgt gtgaacacct acataacggt
    20761 ttcgtttgta atagtcgggc aaaaacactt cttcaacgcg gggaagaata tcgcctgtat
    20821 tgtctcccca taatatttcg agttgaggac ggtcggcagt attacccgga ccggtagcag
    20881 tataggtgat tatagtaact tcaaatgtaa gatcggtaag ctgcctgtaa gtaatttcgc
    20941 cggcacggtt gtgcgttgca aaagcgttgg ttacggcaat gcatacaatt aacagtatgg
    21001 aataaatggt tttcatcata cttataacct tcaaagttac aacatatttt gccatatatc
    21061 cgaattattt accgagcaac gcaaaattta cggaatttaa tcctatagaa ttttcaattt
    21121 ttcgaaacgt tatcatctta gaccgctgtt tatttggaca ggtaaaattt tgtgtttcca
    21181 acagtaccat ttatttatta attctttttt ttttgtaatt ttaacaacaa taacaaaaca
    21241 actcgctgcc atgacaaaaa tttctattct cattccgata cttgtttgct gctggttttc
    21301 tgtccaatcg caaagttttg attactccat cacgttttct gcatcaggag aaacttcact
    21361 tgttgaaact gttactgtgc aaaatatgac acaatgtacc gaagaaacac tgagtggttc
    21421 ttcaaccctg catctcgtgt attcgagcag tattggtgag attgatcaga ataacggttt
    21481 ttttgtttac cccaatccaa ctacagacca atgtattatc agtttccctg tatttgaatc
    21541 aggattggtg caggtgaaac tatcggaaat ctcaggtaag gtactctaca atgatgaatt
    21601 tcaaatgcaa gccggaaaca ataaaatgat tgtaactggt ttaggtttcg gtattttatt
    21661 gttgcagatt aaaactacac aaacgacatt cacaaaaaaa atcatcggaa gtgctgacga
    21721 gaatgttgct ccttctgtta atgttgagtt tttagaaaat tcgggtatta ttaaatcttc
    21781 gcaaaccaaa gagattgttg atatggatta taatcccggt gacattttgc tattcaaagg
    21841 ggtttcaggc aattattcaa attttataac acttgtcgct acggaatcga cgcttgtgac
    21901 gtttcatttt aatgatgcaa ccgacggtga taacaatcat tacgcaacgg tagttatcgg
    21961 tgagcaaaca tggatggccg aaaacctcag aaccacaact tttattgatg gctctcccat
    22021 accaaaaatg gaggatccta ccgaatgggc acaagcttcc ggaccggcat tttgctggca
    22081 ggaaaacgac tcattgactt atgccgaatc gtttggagca ctttataatt ggtttacggt
    22141 tagcaattcg gggaaagacg caatgagtat ttgccctttg ggttggcacg tatctacgga
    22201 agacgactgg gttacattgg gcgagtttct gggaggcaac cctattgcag gaggtaagct
    22261 taaaactttc tgtggtgaat tctggagtga acccaacgaa ggtgccacca atataagcgg
    22321 tgcgagcatc aagggtacag ggttacgaac tatttctggc gttttcggag atacaatgta
    22381 tcaatcctac ttctggacgc caaaacaata tctgcctgaa aacgccagat tcattaatgt
    22441 gagctatatt acatcagaat tgaattttta cgataacgta ttaacctatg gattttcagt
    22501 tcgttgtgta aaagactgat tacccactta ttgtaagtag attcctactt tttaccaatc
    22561 atttggaatc actattgtcc gataaaaata tgaaataaat ttcaattatt gctaagttat
    22621 tgaaaatcaa ctcaaatatt ttacaaaagc ctcatgatta aaaagtaagc ccccttaaaa
    22681 cagtgaaagt tggtgcaatt taacttgctc tttttgccaa tctttatcgg gattttctaa
    22741 aaatctgaac aaatggatgt agttgaacag atgaattttg caaaaactaa ccagattcga
    22801 aaaagcccaa gaccttttta gcattttttg cactactgtc ataagaaggt ttgcaattag
    22861 cacacagtaa atttgtatct tgatggcatt ctcgttatcg ccca
//
LOCUS       DAOOQP010000036        22529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2794_length_22529_cov_6.116179, whole genome shotgun sequence.
ACCESSION   DAOOQP010000036 DAOOQP010000000
VERSION     DAOOQP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2794_length_22529_cov_6.116179"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLK75_05570"
     CDS             <1..320
                     /locus_tag="PLK75_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38751.1"
                     /translation="NLSHNIQEIDCSTDLKKKFLDDVDVFINENYKKYSGLFDDNRLF
                     YSYFFSFNETTDLRPFLFNGLNITDAIVAERMKELAYLFSTNTFDRITNVKNYDCKTC
                     AET"
     gene            complement(465..1871)
                     /locus_tag="PLK75_05575"
     CDS             complement(465..1871)
                     /locus_tag="PLK75_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38752.1"
                     /translation="MWCTISAPNHPQLLNGNNCNKYYFTLPDCFRTLNISNISTTFSN
                     LNRNEKTTFLLHLGYSVFEGTLFGAFLMNEFVFLKSLHGNSYLLGVLFVFSMAVFLLL
                     ILGNQVLSRNGNKRRLLQITALITRLPLFLFLLFPSDISVYSGNSLWHYAFLSIFLLY
                     YLSTPVTYPTINLLLRSNYRISHFGKLFGYANTVNKLFAFIATFVFGWMLEADYSIFR
                     FVYPACGTMGLVSIFCLSLIPYQSTSIEKHRIGLFSSVKKSFNDWVGILRNNREFLSF
                     ELGFMVYGIGFMLTVSAITLYLEGPLQLSYFSYAGYRNFAAVVTILFMPVFSRFIDRI
                     DPRRFARITYWLMLLYILFILLTQWFPESALWHSYQVFPVLLLSFFCLGLFNASMGLL
                     WYIGSSYYCSNDSESGDYQSVHLSFVGIRALIAPLTGVYFYETFGMTFTFFVAIAILL
                     IAIGMTYYSQWKTTVLNK"
     gene            complement(1819..3348)
                     /gene="guaA"
                     /locus_tag="PLK75_05580"
     CDS             complement(1819..3348)
                     /gene="guaA"
                     /locus_tag="PLK75_05580"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOY38753.1"
                     /translation="MQQKILILDFGSQYTQLIARRVRELNVYCEIHPFNHYPEPDNTV
                     KGVILSGSPFSVRDTNAPLPDLTKIKGHIPLLGVCYGAQYLSHYFGGEVAPGNTREYG
                     RANLSFVNRMNPLLNDIPDNSQVWMSHGDTIVSIPESYQIIASTSDVKVAAFQIVGEL
                     SFGIQFHPEVYHSTDGMKLLQNFVVDICGCAQNWTPDSFIETTITDLRTKLGNDNVVL
                     GLSGGVDSSVAASLLHKAIGKNLSCIFVDNGLLRKNEFESVLESYKGMGLNVKGVDAK
                     QRFWNELAGVTEPEKKRKIIGRLFIEVFDEEAHKIANVKWLAQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPEKMNLRVIEPLRLLFKDEVRRVGKALQIPPHILERHPFPGPGL
                     AIRILSDITPEKVAILQEVDAIFISGLKEWKLYNDVWQAGVMLLPVQSVGVMGDERTY
                     ENVVALRAVQSTDGMTADWVHLPYDFLAKMSNEIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            3549..5639
                     /locus_tag="PLK75_05585"
     CDS             3549..5639
                     /locus_tag="PLK75_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOY38754.1"
                     /translation="MKKFLLLVVFCAGVFMAYSQHTELRKQVFEACEQGNVAEVKNLI
                     EKGADISTNENDSTYLVLLYLLNTIEKPELVSFAKSLKEDEVFEILLCATEHGFNVFE
                     QCSAKIPCVAEQWDESSWLWVQVVDPDCISIGYNPYFLIVESGRFHDFEQAIIQNINS
                     GKYSMQSNYGSTPLHIVCEAANSVAVVDALIKNGADVNAVDSTGVTPLLQCMSPDEFK
                     CELCRIDQLEIIKLLLQSGANVNIYKDAYTSLAYSFFEDQYEIAKLLIENGADLSNKE
                     FIYNDSTRSPSAMMYLLWKAFGNCRFNYEILCETDSLEREIVWDLLLTATQHGFNVFE
                     TFSAQLPCVEERYEPESNNYQQVTIPNCITSSYTPYFNITEQGRFKDFEQAIINNINA
                     GIFPKLTSTGSTPLNVACEAANSLAVVDALIKNGADVNAFDSDGCTPLINCVRTSIFD
                     WDSCRVDKLKIARLLIKSGANVNYLIPNEYDYAGHSSLLYASLSKDYALSKLLIENGA
                     DVDGNEQEINDSTRIPSAMFFFLMSVAGNCPVGFEYLAVTDSVDKMQVYELLSMSVSR
                     GFNVFEPIQGSITTVETFYDTIYSYYYQQYKNISVEYLPYAIIANDNILTQFEKVILK
                     QIKQKKYTGTDSYGNSALHYACAYGNSVNIVKAVLKSGADIYATNKNGETPADLAKEN
                     GNIEVYNFISGKMK"
     gene            complement(5744..7201)
                     /locus_tag="PLK75_05590"
     CDS             complement(5744..7201)
                     /locus_tag="PLK75_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOY38755.1"
                     /translation="MSKIFELEPKQVWKHFHSLTQIPRPSKKEAKVIAFMKKFGEDLG
                     LETIVDEIGNVIIRKPATKGMENRKGIIMQTHLDMVPQKNSDNPHDFEKDPIKAYVDG
                     EWVTAEGTTLGADNGMGAAATMAVLESKDLVHGPIEALFTIDEETGMTGAENLKPGLL
                     KGDILLNLDSEDEGELYIGCAGGVDVNVKMEYPQVPSPKGFDAFVLTLKGLRGGHSGM
                     DINVGRANSNKLMARFLNHCIYHFDFKLAKFEGGSLRNAIPREAFATLLIKADEVKKF
                     KAEVTLFDKTYSTEFARTEPDLKLSLAPAEMPKMVAADNFKEKMIKTVFACPHGVERM
                     SDSMPGLVETSNNLAIVKIIDGLFTVNCLVRSSVDSAKMGTMYKIGTIFDLIGADVKF
                     EGAYPGWKPNTDSPILKTMQSVYQKLYGKVPEVKAIHAGLECGILGAAYPNWDMISFG
                     PTIRYPHSPDEKVNIASVGKFWNYLTETLKNVPVK"
     gene            7276..8316
                     /locus_tag="PLK75_05595"
     CDS             7276..8316
                     /locus_tag="PLK75_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOY38756.1"
                     /translation="MKKLAQIFFTGAFLIYFQIYTHAQDNTNPNTGVQWLSLEQAVKL
                     QQAQPKTIMIDMYTDWCGWCKKMEQVTFSNPDIARYINQNFYPVRFNAETRDTVYYRD
                     TMYVNTSQGQKPAHQLAIKLLGGRMSYPTIVYIDDQFHINAVPGYMDAQRIEPLLVYF
                     AEKIYKTANYTDFEINFRKTFFPDTVETNINGKVEWISFADLKAKMQEKPKKVILFIY
                     HDFTTGSRVMGNTTYTHPVIADLINENFYAVKLDVLSTEAFSFFGKEYKNDMVAEAYP
                     HQLAVEYLQPVITLPATVFFEESGKPIYIMRGYVAPWSMEMFIDFIRQNLYRTSTWES
                     FKAGYQGKIERQ"
     gene            complement(8328..8789)
                     /locus_tag="PLK75_05600"
     CDS             complement(8328..8789)
                     /locus_tag="PLK75_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38757.1"
                     /translation="MKNWFLQLSAKSLIVAGLIAATFFFGSCRKDYENHALAFKNITP
                     DTIHVLKYYGSQSPRTFDIAPGVIGKIFETSTEIWLSPSAELKKCSDSIIVQLPDKNI
                     TFAETYTTNYSTNPYYTAAQWEIEVEQYETSSGFGSRIESIYIHYFEITGN"
     gene            complement(8794..10029)
                     /locus_tag="PLK75_05605"
     CDS             complement(8794..10029)
                     /locus_tag="PLK75_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOY38758.1"
                     /translation="MDLIYKISIPILILLSVDCHHGAPDIKGKSQVTTIKNDTVVEDV
                     ITGADQTEAYLPLLLGKRVALVANQTSICGNKHLVDTLVKLGIDVAYVFCPEHGFRGT
                     AGAGDIVGNSTDTETGLSIVSLYGKNKKPQQQYMQNIDIVVFDLQDVGVRFYTYISTL
                     HYVMLACAENNIPLIVLDRPNPNAHYIDGPVLDTACSSFIGMHPVPIVYGMTIGEYAK
                     MINGEKWINTPEPCALTVIPCKNYTHNTPYYLPIPPSPNLPNDRSIQLYPTLCLFEGT
                     CLSVGRGTEMQFQVIGHPLLKSWDKTTFRFTPKPNAGAAKPVLENQECFGFDFRNSLS
                     PFEEGNSRLNISLLVEVWNNFPDKTKFFNKSFVLLAGTKKLQQQIESGMQEDEIRASW
                     QEGIEEFKKIRHKYTIYHD"
     gene            complement(10119..10883)
                     /locus_tag="PLK75_05610"
     CDS             complement(10119..10883)
                     /locus_tag="PLK75_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOY38759.1"
                     /translation="MFFVLSKILQYLLMPVSWLIILVISLLLLKKEKTRKRIIVSIAV
                     ILIVFTNPFLINISMRAWETDTVDKAQLKPSYDYGILLTGMLSYDSKYKRINFWRSSD
                     RLLQTIDLYYDGRIDKILIAGGSGDVLNQEIKEASVLKEYLVHIGIPEEDIITEIHSR
                     NTHENAVETVKILSKLHPGAQCLLITSAFHMRRAHACFKKAGMNCDVYVTDRFGGPVS
                     FKPDKLLVPKVEALGNWTLLIHEVSGFIMYKIMRYC"
     gene            10982..12493
                     /locus_tag="PLK75_05615"
     CDS             10982..12493
                     /locus_tag="PLK75_05615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOY38760.1"
                     /translation="MNNRQIELNPNLLVKYLQKKPCEFTRNDIIDFISGNGIEMLNFR
                     YIAEDGRLKCLSFVINSLEHLEDVLTHGERVDGSSLFSFIEAGSSDLYVIPRYSTAFI
                     NPFEEVPTLEILCAYYNFEGKPLDSSPEYMLEKAHDLFRKKTGFTFKALGELEYYVIS
                     DAEENSEFPATDQKGYHEAHPFTKFDFLRKEAMQLIAQCGGMIKYGHSEVGNFTDGGY
                     YYEQQEIEFLPCEVESAADQLVIAKWILRNLAFKYNLNISFAPKITVGKAGSGMHVHM
                     CIEKNGKSCMIENGRLSNEARRMIAGILEYADALTAFGNTIPTSYLRLVPHQEAPTNI
                     CWGDRNRSTLIRVPLGWVGATDIINQVNPQEKTVAESGNSKQTFELRSGDGSADIHFY
                     LAAIIMAALEGFNMPNALAKAEELYVDVNIFKEEHKNRLAGLKPLPASCFESAIALEN
                     KRNFFENTGVFPKGLIDSTIKKLKAFDDKELSERLYGKHEEIAKLVNKYLHTK"
     gene            complement(12503..13879)
                     /locus_tag="PLK75_05620"
     CDS             complement(12503..13879)
                     /locus_tag="PLK75_05620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY38761.1"
                     /translation="MKKSSKHAPERMLRFSKSWSVFSKYVFFVHRLFYKNIVVEGFEN
                     IPRNKPIIFAPNHQNALMDPMLIISTAEQQVVFLARADIFKNKFIARILMWLKILPVY
                     RIRDGKNNLEKNDESFHQVIEVLEHKLAIGIFPEAQHSDKRHLLALKKGIPRVAFEAE
                     EKNDFNLHIQVIPVGIYYSRYNIFRTIAHVRYGKPIDVTQFKEIYLENEQKGFQALRD
                     AIAAQLEQLAINIRNLQLYDMYESIRALYVKQLIKKFRLGKLTQINRFKADKITIAFL
                     DRYAEDHPEAMEQLREKVAEYEELKRKYSLSDQSIEKPKLKMWRLVASVILLLACLPV
                     FLYGLLNNSIVYFVPKLLVLKIKDKQFHSSVKFGWGVIIGPILYLLQSVVFLLITGSW
                     LYAALYLVSLPVTGFAAQAYFEWDSIIMQDFRKWKLRNLNTETYIQIIKTHKDIIHKL
                     NIISNKYK"
     gene            complement(13876..14841)
                     /locus_tag="PLK75_05625"
     CDS             complement(13876..14841)
                     /locus_tag="PLK75_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOY38762.1"
                     /translation="MFEALKAMDPARLNFNSGGLLILNITIAIIMFGVALEIKPASFK
                     ELFRKPRPVIAGVISQIIFLPIITFLLILAFGGNLTVGVAMGMILVASCPGGNVSNFL
                     STFSKGNATLSVSLTAISTTLAIFITPLNFAFWGKMYQWLGPFSDNPLLKQLEIDPIE
                     MFKTVTILLGIPIVLGMLFNHYFVKATEKIKKPISIFSIVVFMSMVVIMFVNNYQYFL
                     RYIPWILLIVLLHNGLALLTGYGAGTMFRLDERNRRTIAIETGIQNSGLGLVLIFNPN
                     IFPSELPLGGMMIVTAWWGIWHILSGLTIALYWRYRTNRKLKLGQ"
     gene            15044..15523
                     /locus_tag="PLK75_05630"
     CDS             15044..15523
                     /locus_tag="PLK75_05630"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOY38763.1"
                     /translation="MKQKNITIDYLECTTAELDAENIALIQKAMGAAENAYAPYSNFR
                     VGAAVLLENGEIMAAGNQENAAYPSGMCAERTALYYCKALFPEVKIKTLAILALDRNN
                     NILQEPVPPCGACRQVISETIERNRGSFTLLLHGSEKSYIYSDAGSLLPLSFGPSHL"
     gene            complement(15636..16523)
                     /locus_tag="PLK75_05635"
     CDS             complement(15636..16523)
                     /locus_tag="PLK75_05635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOY38764.1"
                     /translation="MAKNEIIDHYKVKEDACLLEALQPMYRGRSKSSLKKLINSGMVL
                     VNGECAKDAAFKVTTGSVITIAKAPKAILKANIYLSPVFEDDNIIVINKMPGVNTNAT
                     NKFDTNSVFHILRNYVQQSNPNNKIFIVHRLDKDTSGVLVFAKSLRIREALQEKWHDK
                     KRMYIAVVEGEVIKEKGSITSWLTETKELKMISSQVRGHGKEAVTRFEVLQQNELYSL
                     LQIEILTGRKNQIRVHMQDLGHPVAGDEKYGAVNNPLKRLCLHAQLLEFDHPVSGERM
                     KFESEAPAGFDKLTRPKQQ"
     gene            16703..17359
                     /locus_tag="PLK75_05640"
     CDS             16703..17359
                     /locus_tag="PLK75_05640"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HOY38765.1"
                     /translation="MKAKHLILLGLTLLAYTASAQEGNNQVAIEFTKFNIVYKGIENP
                     VTIVASDYAAEELSFSVTNGSVETTETPGRIIIKTSAANNSKLELKILANETVLRTIE
                     YVVKTVPDPCFYLGNYRSGSSASIDDIISLPINALKAPIFYYKAEYSVLEFSIRITEG
                     GIPVSYKISGNKISDNPEAAAAVRKGVKSGDNFNFMSFTIKTPSGIRPNVPGIVFTVR
                     "
     gene            17413..18078
                     /locus_tag="PLK75_05645"
     CDS             17413..18078
                     /locus_tag="PLK75_05645"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HOY38766.1"
                     /translation="MKTKQSFLVILLFLCFFGFSQEQSNKVAVEFMQLENVIYKGIEN
                     TVTISASGYSSEDLSFKINNGTIEKTEISGKMIIKTDASVGSTLELHILAKGTEIRVV
                     EYIVNSVPDPLWLLGSYQSGSYVPISDILNTPIKAIKDPHFFYEANYSVKSFCIIIPV
                     KGGVPQKYEINGQKIADNPAAANAARNSFHQGDIISFSDFTIYTPSGLKENVPGVAFI
                     VRE"
     gene            complement(18156..19352)
                     /locus_tag="PLK75_05650"
     CDS             complement(18156..19352)
                     /locus_tag="PLK75_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOY38767.1"
                     /translation="MAKRTIVTMPGDGIGKPVLNETLRVLDAAGFEANYVHGDIGWEF
                     WCKEGNPLPDRTLKLLEEHKIGLFGAITSKPKDKAAAELAPELQGKGYTYYSPIVSMR
                     QHFDLDICMRPCQTLKGNPLNFIRRGANNTIEEPEVDVMIFRQNTEGLYGGVEWTNPP
                     DVVYNGLMTHKKFVDNFAWCPREDLAVSTRIFTRKYTTRIVKAAFEYAKKRGFDKITV
                     SEKPNVIRETSGMMLKIAQEMSKKEYPNIYVEDINIDATMMWLTKNPENYHVIVAGNM
                     FGDIVSDGFAGLIGGLGFATSAQFNPESGVCVFEPTHGSAPKYADYPVSIVNPIAMIL
                     SAVMMLEHIGEDEKAQRIRKAVAKVVADGKVRAYDMLKMKGSPDVINQGAASTKQMAD
                     AIIAAL"
     gene            complement(19496..20230)
                     /locus_tag="PLK75_05655"
     CDS             complement(19496..20230)
                     /locus_tag="PLK75_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38768.1"
                     /translation="MKFCCIFMVLLGAISMTVYSQNSTHPVSIALYSNFNLNSNYITH
                     GQGPSEGTIHTEFEREFYGFNFSPAVEINTDKGNYHSVEISRLAYNNNYNKEYYIVDS
                     TGAISQVLSASTAQAFNCFLRYEYTITLFKKQNWEKFGTAIGFSATPFFRWSKSNSSI
                     SSEFPVTKTGTGIYLSVIPRLTYSFSEKWFIDLNVPLALVTAQYNSIKTDNPVLPVNQ
                     RNTATFDFHQGPIGVAVRLGVGYRFR"
     gene            20807..21667
                     /locus_tag="PLK75_05660"
     CDS             20807..21667
                     /locus_tag="PLK75_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38769.1"
                     /translation="MKNLHLLIMIFSITPSFSQEISLGDNLYTEFNKISAPYFFEMSN
                     NTGWIEGEGNFYIGDMPILGDCNVIYMALKDTTLFGLFVKKPQATFMFDSQGDSILDV
                     SPGFFMHPFWVVKNNTKISPDDKKIIEVFNNIYEKTLQSDYFDNADNPFSEFVKYRTD
                     TSLSNRHIAYLFEYVLLSSTPSSENDTPEFAKFSLDLNLSLASECLSLFHVIPEIVYV
                     YLGEAFIACGMTDKAIENYEIALQLYPDCIPCKVYNCVLEKDSVKKKEKSKELRKKHP
                     NHWLVNELIN"
     gene            complement(21724..>22529)
                     /locus_tag="PLK75_05665"
     CDS             complement(21724..>22529)
                     /locus_tag="PLK75_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOY38770.1"
                     /translation="FERSNYKVSRKTIYSDKLPAAFDGTTIAQISDFHAGSFHNRDEV
                     IKGIEIIKDLKPDIFVFTGDMVNNLAKEAVPWIPVLRDIQAPLGKFSVIGNHDYGDYV
                     RWKNQEAKDANLAQLVRHEQQAGFDVLINEHRAIVKGSDTIYVAGVENWGHNFIRQGD
                     LPKSLNGLSDKDFIVLLSHDPSHWDWQVKTYPVPVALTLSGHTHGFQFGIEWGKFKWS
                     PGQYLYPNWSGLTEENGKYLYVNQGFGYIAFEGRIGMPPEITLITLKRR"
BASE COUNT         6643 a         4445 c         4594 g         6847 t
ORIGIN      
        1 ctaatctatc tcataatatc caagagattg attgttcaac agatttaaag aaaaagtttt
       61 tagacgatgt tgatgttttt attaacgaga attataagaa gtattccggt ttgtttgatg
      121 ataatcgctt gttttattcc tattttttta gttttaatga aactacagac ctgagaccgt
      181 ttctatttaa tggcttaaat attactgatg caatagtcgc tgaaagaatg aaagagcttg
      241 cttacctgtt tagcacaaat acttttgaca gaattacaaa tgtgaaaaac tatgattgca
      301 agacttgtgc tgaaacgtaa aattgacaag atatcgtaat ggactggtta ttattatgcc
      361 tcgttgttga aacaaattta gaaattagta gaaagcaata tcaaagatat tttcattatt
      421 ccgctatctc atactttata ttttaagaat attaagcaaa tgcgctactt attcaatact
      481 gtggttttcc attgtgagta atatgtcatt ccgattgcaa tcagtagaat tgcaatggcg
      541 acaaaaaaag tgaaagtcat tccgaaggtc tcgtagaaat atacaccggt aagaggcgct
      601 atcaatgccc ttattccaac gaaggaaaga tgtacgcttt gataatcgcc actttcacta
      661 tcgttgctgc aataatatga tgaaccgata taccagagta atcccatact tgcattgaaa
      721 agtcccagac agaaaaacga cagtaaaagc acaggaaata cctgatagga atgccataaa
      781 gcagattcag ggaaccactg cgtcaacaaa ataaataaaa tgtaaagcag catcagccag
      841 taggtaattc gggcaaacct gcgcgggtcg atacggtcga taaacctact gaataccggc
      901 ataaacagaa tggttacaac cgctgcaaaa ttccggtaac cggcatagct gaaatatgat
      961 aactgcaatg gtccttccag ataaagtgtt attgccgaaa cagtaagcat gaatccgatc
     1021 ccgtacacca taaaacccag ctcaaaactc aggaattcgc gattattgcg caaaatgcca
     1081 acccagtcgt taaacgattt ttttaccgat gaaaacagtc ctattctatg tttttcaatt
     1141 gaagtggatt ggtatggtat aagcgaaagg caaaaaatag aaactaggcc catcgtacca
     1201 catgcaggat acacaaaccg gaatatcgaa taatctgcct cgagcatcca accaaaaaca
     1261 aatgtcgcaa tgaaagcaaa cagcttgttt acagtgttgg catagccaaa taatttgcca
     1321 aaatgcgaga ttcggtaatt cgatctgagg agcaggttaa ttgttggata agtgaccggt
     1381 gtgctgagat agtataacag aaaaatactt aaaaatgcat agtgccacaa tgagttgccg
     1441 gaatacacgc taatatccga tggaaacagt aaaaataaaa acagcggcag acgtgtgatc
     1501 aacgctgtaa tctgcagcag acgcctttta ttcccgttac ggctcagaac ttgattgccc
     1561 agtatcagta gcagaaaaac tgccatactg aacacaaaca gcaccccaag caggtacgaa
     1621 ttgccatgaa gactttttaa aaacacaaat tcattcatca gaaatgcccc aaacagggtg
     1681 ccctcgaaca ctgagtaacc caaatgcagc aaaaatgtcg tcttttcgtt gcggttcagg
     1741 ttactgaatg ttgtgcttat attgctgata ttcaatgttc tgaaacaatc gggtaaagtg
     1801 aaatagtact tattacagtt attcccattc aatagttgcg ggtggtttgg agctgatatc
     1861 gtacaccaca cggttcacac ctttaacttt gttaatgatc tcattcgaca ttttggccag
     1921 aaaatcataa ggaaggtgta cccagtcagc cgtcatacca tcggtcgatt gtacggcacg
     1981 taaagcaacc acattttcgt aagtgcgttc atcacccata acgcctaccg attgaaccgg
     2041 aagtagcatt acgcctgcct gccacacgtc gttgtatagc ttccattctt ttaatccgct
     2101 gataaaaatt gcatctactt cctgcagaat agccactttt tcgggggtaa tatcgctgag
     2161 tatcctgatg gctagtccgg gacccggaaa aggatggcgt tctaaaatgt gtggaggtat
     2221 ctgtaaggct tttccaacgc ggcgcacctc atccttgaat aacaagcgca aaggttcaat
     2281 taccctgagg ttcatttttt cgggcaatcc gccaacatta tggtgcgact taatggtagc
     2341 tgaaggtccg tttaccgaca ccgattcaat tacatcgggg taaatggtac cctgtgccag
     2401 ccattttacg ttcgcaattt tatgtgcttc ttcatcgaaa acttcaataa acaacctgcc
     2461 gatgatctta cgcttttttt caggctcggt tacacctgcc agttcattcc agaatcgttg
     2521 ctttgcatca acacctttta cgttaagccc cataccttta taggattcga gcacagattc
     2581 gaattcgttt ttacgcaaca atccgttatc aacaaaaata cagctcagat ttttcccaat
     2641 ggctttgtgg agcaaactag ctgctaccga agaatctacg ccccccgaca agcccagcac
     2701 cacgttgtcg ttgccaagtt ttgtgcgtaa atcagtaatg gtagtctcaa taaacgaatc
     2761 aggcgtccaa ttctgagcac acccacagat gtccactaca aaattctgta gtagcttcat
     2821 tccgtcggtg gaatgatata cttccggatg aaattgaatg ccgaagctga gttcgcctac
     2881 aatctgaaaa gcggcaactt ttacatcgct ggttgatgca attatttggt acgattcggg
     2941 gatagaaact atcgtatcgc catgcgacat ccatacctgc gaattgtcgg gtatatcgtt
     3001 gagcaaagga ttcatgcggt tcacaaaact taggttggcg cgcccgtact ctctggtgtt
     3061 tccgggagca acttctccgc cgaaataatg cgaaaggtat tgtgctccat agcaaacccc
     3121 cagcagaggg atgtgccctt taatttttgt taaatccggc aacggtgcgt ttgtatctct
     3181 taccgaaaaa ggactgccgc tgagaataac acctttcacg gtattatcgg gttctgggta
     3241 gtgattgaaa gggtggatct cgcagtacac attaagctct cttacacgcc gggcaatcag
     3301 ttgggtgtat tgtgagccga aatcgagaat cagtattttt tgctgcatct gggcgatttt
     3361 gtctgcaaat atagcagaat tgaagcttca aaatgtgcca tatcccgatt tgttaagaag
     3421 ttttgaaaac aaatatttca ataaaatatt aaatagaata ttaggctaat tatataatat
     3481 ttggtattat tgctgtaaga taatcaggta tttatagaaa ccattttttt aacctaaagc
     3541 ttattattat gaaaaaattc ttattgcttg tcgttttttg tgcaggtgtc tttatggctt
     3601 attctcagca taccgaactg cgtaagcaag tatttgaagc ctgtgaacaa ggtaatgttg
     3661 ctgaggttaa aaacctgatc gagaaaggag cggacatttc tacaaatgag aatgattcca
     3721 cgtatttggt attgctctat ttgctgaata ctattgaaaa acctgaattg gtaagtttcg
     3781 ccaaatccct gaaagaagac gaagtatttg aaattttgtt atgcgctact gagcatggtt
     3841 ttaatgtttt tgagcaatgt agtgcaaaaa ttccatgtgt tgccgaacaa tgggatgagt
     3901 catcatggtt atgggtacaa gtggtggacc ccgattgcat ttccataggc tacaacccat
     3961 attttttaat agttgaatcg ggcagatttc atgattttga gcaagcaatt attcagaata
     4021 ttaattccgg aaaatattct atgcaatcga attacggcag cacacccttg catattgtct
     4081 gcgaggcagc gaattctgtt gctgtggttg atgcgttgat aaagaatggt gcagatgtga
     4141 atgcggttga ttccaccggt gttactcctc tgctgcagtg catgagccca gatgaattca
     4201 agtgtgaatt atgcagaatt gatcagttag aaattataaa actactgctt cagtcaggtg
     4261 caaatgtaaa tatatataaa gatgcataca cttctttagc ctactctttc tttgaagatc
     4321 aatacgaaat tgcgaagttg ctgattgaaa acggggccga tttgtcaaac aaagaattta
     4381 tttataatga tagtacacgg agcccatcgg ctatgatgta cttgctgtgg aaagcctttg
     4441 ggaattgtcg atttaattat gaaattttat gcgaaaccga ctcgcttgag agggaaattg
     4501 tttgggattt gttgcttact gcaacgcaac atggttttaa tgtttttgaa accttttcag
     4561 ctcagctacc atgcgttgaa gagcgatatg aaccagaatc taacaattat caacaggtga
     4621 ccattcccaa ttgcattact tcaagctaca caccttattt taatattact gagcaaggaa
     4681 gatttaagga ttttgagcaa gcaataatta ataatattaa tgcaggtatt ttcccgaaac
     4741 taacaagcac tggcagcacc cccttgaacg tcgcctgcga ggcagcgaat tctcttgctg
     4801 tggttgatgc gttgataaaa aatggtgctg atgtgaatgc gtttgattct gatggttgta
     4861 ctcctcttat caattgtgta aggacgtcga tttttgattg ggattcatgc agggtggata
     4921 aattgaaaat tgcaagattg ctcatcaaat cgggagcaaa tgtaaattat ctgattccaa
     4981 atgaatatga ctacgccggt cattcatcac tactatatgc aagtttaagc aaagactatg
     5041 cattgtcgaa attgctcatc gaaaacggag cggacgtgga tggtaatgag caggaaatta
     5101 acgacagtac gcgcattcca tcggctatgt ttttctttct gatgtctgtt gccggtaatt
     5161 gcccagtagg atttgaatat ttagctgtga cagactctgt tgataaaatg caagtgtacg
     5221 agcttttatc aatgtcggtc agtcgtggat ttaatgtgtt tgagcccata caaggcagta
     5281 ttactactgt tgagacgttt tacgatacta tctattccta ttactatcaa caatacaaaa
     5341 atatttcagt ggaatattta ccctatgcta ttattgcaaa tgataatata cttactcaat
     5401 tcgagaaagt aatccttaag caaatcaaac aaaaaaaata caccggcacc gatagctatg
     5461 gcaatagtgc cttgcattat gcctgtgctt atggaaattc tgtgaatatc gttaaagcgg
     5521 tgcttaaaag cggagccgat atatatgcta caaacaaaaa cggggaaaca cccgctgatt
     5581 tagcaaaaga aaatggcaat attgaagtgt ataattttat atctggtaag atgaagtaaa
     5641 ttgcctgtaa tgaattataa aatttgctaa cctctggatt gataataaaa aaaagaccat
     5701 caaaacacaa gtctgatggt cttattaagg tgttatggtt tatttatttc accggaacat
     5761 ttttcaaggt ttcagtgagg tagttccaga atttaccaac gctggcaatg ttcacttttt
     5821 cgtcgggtga atgcggatag cggatggtgg gaccaaatga aatcatatcc cagttcgggt
     5881 aggcagcacc cagaatacca cattccaaac cggcatgaat agccttaact tcgggaactt
     5941 tcccatagag tttctgatat accgattgca tggttttcag aattggtgaa tcagtgtttg
     6001 gtttccagcc cggatatgca ccttcgaatt taacatcagc accaatcagg tcgaaaattg
     6061 ttccaatctt gtacatggta cccattttgg cagaatcaac cgaactacga accagacaat
     6121 ttaccgtgaa taagccatcg ataattttta cgatagccag attgttcgac gtttccacaa
     6181 ggcccggcat actgtcgctc atgcgttcaa ctccatgggg gcaggcaaaa acggttttaa
     6241 tcattttttc tttgaagtta tcggcagcta ccatttttgg catttcggca ggagcaaggc
     6301 ttaatttcag atcaggctca gtgcgggcaa attcggtgct ataggtttta tcgaacaacg
     6361 taacttcggc tttgaacttc tttacctcat cggcttttat caataaggtt gcaaaagctt
     6421 cacgcggtat ggcattacgc agacttccgc cttcgaattt tgccagtttg aaatcgaaat
     6481 gataaatgca atggttcagg aaccttgcca tcagcttatt gctattagca cgtccaacat
     6541 tgatgtccat accagagtga ccacctctca gtcctttgag agtcagtaca aatgcatcaa
     6601 agccttttgg tgacggtact tgcgggtatt ccatcttaac atttacatca acgccaccgg
     6661 cacagccaat gtacagttca ccttcatctt cagagtcgag attcagcagt atatcacctt
     6721 tcaataaacc tggtttcagg ttttcggcac cggtcatacc ggtttcttca tcaatggtaa
     6781 atagagcctc aatgggtcca tgcacaaggt ctttactttc gagaactgcc attgttgcag
     6841 ctgctcccat accattatca gcacccaggg tggttccttc ggcagttacc cattctccgt
     6901 cgacgtaggc tttgatggga tctttttcaa aatcgtgagg gttgtcggaa ttcttctgcg
     6961 gcaccatatc taggtgggtt tgcataataa ttcccttgcg gttttccata ccttttgttg
     7021 cgggtttacg gataattacg ttaccgattt catcaacaat tgtttccaat cccaggtctt
     7081 ctccgaattt tttcatgaag gcaattactt tggcttcttt tttggagggt ctcggaatct
     7141 gggtaagact atggaaatgt ttccatactt gtttgggttc taattcaaaa atcttgctca
     7201 tatttgtttg ttattggtta tgttattaaa agaatggaaa attacttatt tttatctgtt
     7261 ttgaaaacaa aaactatgaa aaaattagct caaatatttt ttactggagc ctttctcatt
     7321 tattttcaga tatatacaca tgctcaggat aatacaaatc ccaataccgg tgtgcaatgg
     7381 ctcagcttgg aacaagcggt aaagcttcaa caggcgcagc ctaaaacaat tatgatagat
     7441 atgtacaccg attggtgcgg ctggtgtaaa aaaatggaac aggttacatt cagcaatcct
     7501 gatattgcac gatatatcaa tcagaatttt taccctgtgc ggtttaatgc cgaaacccgc
     7561 gataccgtgt attaccgcga taccatgtat gtaaatacca gtcagggaca aaaacctgct
     7621 catcagcttg caatcaagtt actgggtgga cgtatgtcgt atcctacaat tgtgtatatc
     7681 gacgatcagt ttcatattaa cgctgtgccc ggatacatgg atgcgcagcg aattgaacca
     7741 ttacttgtgt attttgccga aaaaatttat aaaactgcca attacaccga ttttgagata
     7801 aatttcagga agaccttttt ccccgatacc gttgaaacca acattaatgg aaaagtggag
     7861 tggataagtt tcgctgacct gaaagcaaaa atgcaggaga agcccaaaaa agtgatacta
     7921 tttatttacc acgattttac tacagggagc agggtcatgg gcaacacaac ctatacacat
     7981 ccggtaattg cggacctaat taacgaaaac ttctatgcag ttaaattaga cgttctgtcg
     8041 accgaagcat tttctttttt tggcaaggaa tacaaaaacg atatggttgc ggaagcttac
     8101 cctcatcaac tggctgtgga atacctgcaa cctgttatta cattgcctgc tacggtgttt
     8161 tttgaggaaa gtggtaagcc catttatatt atgcgaggat acgttgctcc ttggtcgatg
     8221 gagatgttca ttgattttat ccgccaaaac ctttatagaa caagcacctg ggagagtttc
     8281 aaagctggtt accaaggaaa aattgaaagg cagtagccaa attagaatta gttgcctgtg
     8341 atttcgaagt agtgaatgta aatggattca attctgcttc caaatccaga agatgtttca
     8401 tattgttcaa cttcgatttc ccattgtgcg gccgtataat agggatttgt gctgtaattt
     8461 gtcgtatagg tttcggcaaa tgttatattt ttatccggaa gttgaacaat gatggagtcg
     8521 ctgcattttt ttagctctgc cgaaggacta agccatattt cggtgctggt ttcgaaaatt
     8581 ttaccaatta ccccgggtgc tatgtcgaat gtgcgtggcg attgcgaacc atagtatttt
     8641 aatacatgaa tcgtatcggg tgtaatattt ttaaatgcaa gcgcatgatt ttcgtaatct
     8701 ttgcggcatg atccgaagaa gaatgtcgca gcaatcaacc ctgcaacaat cagacttttt
     8761 gcagaaagtt gtaaaaacca atttttcata gattcagtca tgatatatag tgtatttatg
     8821 tctgattttt ttgaattctt caattccttc ttgccatgag gctctgattt catcctcctg
     8881 cataccgctt tcaatctgtt gctgcagttt ttttgtgccc gcgagtaata caaacgattt
     8941 gttaaagaac ttggttttat ccgggaaatt attccaaact tcgacaagca gactaatgtt
     9001 aagacgggaa ttaccttctt caaaggggct caacgaattt ctgaaatcga atccgaagca
     9061 ttcctgattt tcgagtaccg gcttggctgc tcctgcgttt ggttttgggg tgaaacgaaa
     9121 tgttgttttg tcccacgatt tcagcaaagg gtgtccgatt acctggaatt gcatttcggt
     9181 acccctgcct acgcttaagc aggtcccctc aaaaagacaa agcgtgggat ataactgaat
     9241 ggatcggtcg ttggggagat ttggtgaagg tggtataggc agatagtacg gtgtgttgtg
     9301 cgtatagttt ttacatggta tcacagtaag tgcacaaggt tcaggtgtat tgatccattt
     9361 ctcgccattt atcatttttg catattcgcc gatagtcata ccataaacaa taggcaccgg
     9421 atgcattccg atgaatgaag aacaggcagt atcgagtacc ggcccgtcga tataatgcgc
     9481 attcgggttt ggccggtcaa gaacaataag aggaatatta ttttcggcgc aggccagcat
     9541 tacataatgc aatgtggaaa tatatgtata gaaacgcacc cccacatcct gcaggtcgaa
     9601 aacaactatg tcaatattct gcatatattg ttgttgcggc tttttgtttt ttccatagag
     9661 cgaaacaatg gaaagacccg tttcggtatc agtactgtta ccaacaatgt ctccggcacc
     9721 ggctgtccct ctgaaaccat gttccggaca gaaaacatat gctacatcaa tacccagctt
     9781 aactaacgtg tcgaccagat gtttgttacc gcaaatactg gtttgattgg caaccaaagc
     9841 aaccctcttt cctagcaaaa ggggcagata ggcttcggtt tggtcggctc cggtaataac
     9901 atcctcaaca acggtgtcgt ttttgatggt agtgacctgc gatttaccct taatatccgg
     9961 cgcaccgtga tggcaatcaa ccgaaagcaa aatcaggatg gggatagata ttttataaat
    10021 taaatccata ttcatgataa aattacaagt aatgagaatg caaatgaaac taaccccatt
    10081 aaaaatatga acgataagtg tttaaaaatg ttagtatttt agcaataacg cattatttta
    10141 tacatgataa atcctgaaac ttcgtgaata agcagtgtcc agttacccaa agcttctact
    10201 tttggaacca acagtttatc gggtttaaag cttacagggc ctccaaacct gtcggtaaca
    10261 tacacgtcgc aattcatacc agctttttta aagcaagcat gagcgcgtcg catgtgaaac
    10321 gcactggtta taagaagaca ttgtgctccc ggatgaagtt tgctcagtat tttcactgtt
    10381 tcaacagcat tttcgtgggt attgcgtgag tgtatctcgg ttatgatatc ctcttcagga
    10441 attccaatgt gaactaagta ctcctttaaa acggatgctt cttttatttc ctgatttaaa
    10501 acatctcccg agcctccggc aataagtatt ttatcaattc ttccatcgta atacagatca
    10561 atggtttgaa gaagccggtc gctgctgcgc cagaagttga ttctcttgta tttggagtcg
    10621 tacgacaaca ttccggtaag aagaattcca taatcatagc tcggcttcag ctgcgcttta
    10681 tcaaccgtat cggtttccca cgcccgcata ctgatgttta ttaaaaacgg gttcgtaaaa
    10741 acaattaata ttactgcgat agatacaata attcgttttc ttgttttctc tttttttaat
    10801 aatagcagtg atataaccaa aattattagc catgacaccg gcatcagaag atactgaaga
    10861 attttcgaca atacaaaaaa catattatac agcctttttt tgtttaatta tagcatgcgt
    10921 catatcgtat tttaccaata atttgtaaat ttaccgactt atgcgaaaat agtttaaaat
    10981 tatgaataat cgacaaattg aactgaatcc gaatttattg gttaaatacc ttcaaaagaa
    11041 accatgtgaa tttacccgta acgacattat tgattttatt tcaggcaatg gcatcgaaat
    11101 gctgaatttc cgctatatag ccgaagatgg aagactgaag tgccttagtt ttgtaatcaa
    11161 tagccttgaa catctcgaag atgttcttac acacggggaa cgtgtcgacg gtagcagtct
    11221 gttttcgttt atcgaggcgg gttcaagcga tttatatgta ataccccgtt atagtactgc
    11281 atttatcaac ccgtttgaag aagtgccgac acttgaaatt ctatgtgctt actataactt
    11341 cgaaggcaag ccactcgaca gcagtcctga atatatgcta gagaaagccc acgatctatt
    11401 tcggaaaaaa acagggttta catttaaagc attgggtgag cttgagtact atgtaatttc
    11461 cgatgctgaa gaaaacagcg aattccctgc aactgatcag aaaggctacc acgaggcgca
    11521 cccttttaca aagtttgatt ttctgcgtaa agaagctatg caattgatag ctcagtgtgg
    11581 tggtatgata aagtacgggc actccgaagt tggtaatttt accgatggtg gttattatta
    11641 cgagcaacag gagattgagt ttctgccttg tgaagtggaa tcagcggccg atcagttggt
    11701 tattgcaaag tggatactcc ggaacctggc ttttaaatat aatctgaata taagtttcgc
    11761 gccgaaaatt actgtaggaa aggcaggcag cggaatgcat gtgcatatgt gtatcgaaaa
    11821 gaacggcaaa agctgtatga ttgagaacgg tagactcagt aacgaagcac gacggatgat
    11881 agccggtatt ctagaatatg ccgatgccct gactgctttc ggtaacacta tcccgacgtc
    11941 ttatttgcgt ttggtgcccc atcaggaggc tccgacaaat atatgctggg gcgacagaaa
    12001 ccgcagtaca cttatcaggg ttccccttgg atgggtggga gcgaccgata ttattaatca
    12061 ggtgaatcct caggaaaaaa ccgttgctga aagtggaaac tcaaaacaaa cattcgaatt
    12121 gaggagcgga gacggatctg cagatattca tttttactta gctgcaatca ttatggctgc
    12181 actcgaaggg ttcaatatgc ccaatgctct cgcaaaggct gaggagttat atgtggatgt
    12241 taacattttc aaggaagaac ataagaatcg gcttgccgga ttaaaaccgc ttcctgcatc
    12301 atgtttcgaa tcggctattg cactcgaaaa caaacgaaac ttttttgaga ataccggcgt
    12361 tttccccaaa gggcttattg acagcactat taaaaagctg aaagctttcg acgataaaga
    12421 actgagcgaa cgattgtatg gaaagcatga ggagatagct aaacttgtaa acaaatacct
    12481 gcataccaaa taaattcagt gtttatttgt atttgttcga aataatattc agtttgtgaa
    12541 tgatatcctt atgtgttttt attatctgaa tgtaggtttc ggtattcagg tttctaagtt
    12601 tccattttct gaaatcctgc attataatag aatcccattc aaaatatgcc tgtgctgcaa
    12661 accccgttac aggcaaactg actaagtata aggcagcgta tagccacgaa ccggttatta
    12721 aaaggaaaac tacagattgc aacaagtata gtatcggacc tatgattact ccccagccaa
    12781 attttaccga actatgaaac tgtttgtctt taattttaag caccagtagt tttggaacaa
    12841 agtaaacaat cgaattgttg agtaacccgt acagaaatac cggcaggcag gcaagtaata
    12901 atattaccga agctaccagc ctccacattt ttagctttgg tttctctata ctctgatccg
    12961 ataaagagta cttccgtttc agctcttcat attcggcaac tttttcacgc aattgttcca
    13021 tagcttccgg atggtcttcg gcataccggt cgagaaaagc aatggtaatc ttgtcggctt
    13081 taaaacggtt gatctgagta agcttaccca gacggaattt tttaatgagt tgctttacgt
    13141 acaaagccct gatgctttcg tacatatcgt acagttgcag gttccttata tttatggcaa
    13201 gttgttcaag ctgggctgca atggcatcgc gtagggcctg aaaacctttc tgttcgttct
    13261 ccagatagat ttctttgaat tgcgtaacat caattggttt gccgtaccta acgtgggcta
    13321 tcgtgcgaaa gatattataa cggctgtaat atataccaac aggaattacc tgtatgtgaa
    13381 gattgaaatc gttcttttct tctgcctcga atgccacacg cgggattcct tttttaagcg
    13441 caagcaaatg ccttttatcg ctgtgttgtg cctcgggaaa aataccgatt gccaatttat
    13501 gctcgagtac ttcaattacc tgatgaaaag attcatcgtt tttttcaaga ttgtttttgc
    13561 catcacgaat gcggtacacc ggaagtattt taagccacat gagtatgcga gcaatgaact
    13621 tatttttgaa aatatcggct ctggcaagaa ataccacttg ttgttctgcg gttgaaatga
    13681 ttaacattgg atccatcagt gcattttgat ggttgggtgc aaaaataatc ggtttgttgc
    13741 ggggaatatt ttcaaaccct tcaactacta tgtttttgta gaataaacgg tgtacgaaaa
    13801 aaacatactt cgagaaaaca ctccacgatt tcgaaaaccg cagcattcgt tcgggagcat
    13861 gtttactact ttttttcatt gcccaagttt gagttttctg ttggtgcgat aacgccaata
    13921 caatgctatg gtgagcccgg atagtatatg ccatattccc caccatgctg ttactatcat
    13981 cataccaccc aggggtagtt ccgacgggaa aatattgggg ttaaaaataa gcaccaaccc
    14041 caggccggag ttctgaatac ctgtttcaat tgcaattgtc ctgcggtttc tttcgtcaag
    14101 cctgaacata gtacctgcac cgtatccggt aagcagtgcc agtccgttgt ggagtaaaac
    14161 tattagtaaa atccatggaa tatacctgag aaaatactga taattattca caaacattat
    14221 taccaccatc gacatgaaaa ccacaatgct gaaaatgctg atgggttttt ttattttctc
    14281 ggtagctttc acaaagtaat gattgaaaag cataccaagc acaatgggta ttccaagcaa
    14341 tatagttacg gttttgaaca tttctatcgg atcaatctca agttgcttta ggagggggtt
    14401 atcgctgaaa ggtcccagcc actgatacat tttcccccag aatgcaaaat ttagtggtgt
    14461 gataaatatg gcaagtgttg tggaaatggc ggtcagactc accgatagcg ttgcatttcc
    14521 tttcgaaaaa gttgacagaa aattcgaaac attgcccccg gggcatgaag cgacgagtat
    14581 catacccata gcaactccaa cggtaaggtt tcccccgaaa gcaagaatca gaagaaatgt
    14641 aatgatcggt aaaaaaatta tctgagaaat taccccggca atcaccggtc ggggttttct
    14701 gaaaagttcc ttaaaacttg ccggtttaat ttcaagtgca acaccaaaca ttataatggc
    14761 aatggtgata ttcaaaataa gcagtcctcc cgagttgaaa tttagtctgg caggatccat
    14821 tgctttcagt gcttcgaaca taacactact gtttttggca aaactaatat ttattcctga
    14881 aacttcgatt tcaaaaatgc acaggaacag ttaagataat tctttttcac attgctgccg
    14941 gtgcaacgga tgtgtttacc tgaaataggt tacagacagc tttttatgtc ggatatgccg
    15001 aaattcaagt tattgtatta actttgtaga agtgtttatc gaaatgaagc aaaaaaacat
    15061 taccatcgat taccttgagt gtactactgc cgaacttgat gcagaaaata ttgctttaat
    15121 acaaaaagca atgggtgcgg ctgaaaatgc ctatgcccca tattcgaatt ttagggttgg
    15181 tgcagcggtg ctgctcgaaa atggcgaaat tatggctgcc ggcaatcagg aaaatgccgc
    15241 gtatccatcc ggtatgtgtg cagaacgcac cgcactctat tattgcaaag cacttttccc
    15301 ggaagttaaa ataaaaacac tggcaatact tgcactcgac agaaataaca atatactgca
    15361 ggagcctgta cctccatgcg gagcatgccg ccaggtaata tccgaaacaa tagagcgtaa
    15421 tagagggagt tttacgctac ttctgcatgg ctcagaaaaa tcatatattt attccgatgc
    15481 gggaagtcta ttgccgctaa gttttggtcc atcccatctt tgatctgcga ttgcagatgg
    15541 tatactgttc taacaggcat cattgccaat agactaaagg taatgactga ttccttcagg
    15601 ttttaataac aaagtatttt cgtgaattcg ttgtattact gttgctttgg tctggtaagc
    15661 ttatcgaacc cggcaggagc ttccgattca aacttcattc gttccccgct aacgggatga
    15721 tcgaattcca gcaattgggc atgaagacac aagcgtttca gcgggttgtt aactgcaccg
    15781 tatttttcat cacctgccac cgggtgtccc aaatcctgca tatgcaccct gatctgattt
    15841 ttccgaccgg taagaatttc tatctgcagc aaactataca attcgttttg ctgcagtacc
    15901 tcgaatcggg taactgcttc cttgccatga ccgcgaacct gcgatgaaat cattttcagt
    15961 tctttcgtct cggtaagcca cgacgtaatg cttccttttt ccttgataac ctctccttca
    16021 acaacagcaa tgtacatccg ctttttatcg tgccattttt cctgtaatgc ttcgcgtata
    16081 cgcagacttt tggcaaatac caatactccg gaagtatctt tgtcgagcct gtgaacaatg
    16141 aaaatcttat tgttcgggtt gctttgctgc acatagttcc tcagaatatg aaaaaccgaa
    16201 ttcgtatcga atttattggt agcattggtg ttcactcccg gcattttgtt tatcacgata
    16261 atattatcgt cttcgaaaac cggactgaga tatatattcg ctttcagtat ggctttcggc
    16321 gctttggcaa tagtaattac cgaacctgtt gtaactttaa aagcggcatc ttttgcacat
    16381 tctccgttaa ccaacaccat cccagaatta attagttttt ttagagaaga tttacttctt
    16441 ccgcgataca tcggttgtag cgcttcaagt aagcaggcat cttcttttac tttgtagtgg
    16501 tcgataattt cgttttttgc catttaaatt gagcatatca tacatacgaa cttcgtctgc
    16561 aagatacaaa aaaccttaag atagtgtatg ctatgatatt cagtttagaa tcacaggttt
    16621 attaaaaaaa atatacttcc ttgttttata ttaattataa tattatatat ttgtagatat
    16681 aattcatcta aatttattta ttatgaaagc aaaacacttg atcttgttag gtttaacctt
    16741 acttgcctat actgcaagtg ctcaagaggg taacaatcag gttgccattg agtttacgaa
    16801 gttcaacatt gtttacaagg gtattgaaaa tccggttaca atagttgcat cggattatgc
    16861 tgccgaagaa ttgagttttt ctgtgacaaa tgggtctgta gagacaacag aaactcccgg
    16921 tcgaataatt attaaaacct ctgcagcgaa taattcaaag ttggaattga aaattttggc
    16981 aaatgaaact gtgctgcgta ctatagaata tgttgtaaaa acagttcctg acccttgttt
    17041 ttatcttggg aattatcgct ctggcagcag tgcttcaata gatgatatta ttagcctccc
    17101 gataaatgcc cttaaagccc cgatattcta ttataaggct gaatactcgg tgttggaatt
    17161 ttctattaga ataaccgaag gaggtattcc agtatcttat aagattagtg gcaataaaat
    17221 atcggataac ccggaagctg ctgcagcagt gcgtaaaggg gttaaatcag gagacaattt
    17281 taattttatg tcttttacaa ttaaaacgcc atcaggaata aggccaaatg ttccagggat
    17341 agtttttact gtccgataat ggtattatct taatttgaca tattattgtt ttaattacca
    17401 aattttataa acatgaaaac aaaacaatct ttcttagtga ttttattatt tctttgcttc
    17461 tttggatttt cgcaggaaca aagcaataaa gttgccgtcg aattcatgca gttggaaaat
    17521 gttatctaca agggcattga gaacactgta acgatatcgg catcaggcta ttcatcagaa
    17581 gacctgagtt ttaaaattaa taatggaact attgagaaaa ccgaaatctc cggcaaaatg
    17641 ataataaaaa ctgatgccag tgttggctca accctggaat tacacatttt ggcaaaggga
    17701 actgagatac gcgtcgtgga gtatattgtt aattctgttc ctgatccgct gtggctgtta
    17761 ggtagttatc aatctggaag ttatgttccg ataagtgata ttttaaatac accgataaaa
    17821 gccataaaag acccacactt tttttacgaa gcgaattact ccgttaagag tttttgcatt
    17881 attatcccgg taaaaggtgg agtgcctcag aaatatgaaa ttaacggaca gaaaattgcc
    17941 gataatccag ccgcagctaa tgcagctagg aatagttttc accagggcga tattatcagc
    18001 ttttcagatt ttactatata tacccccagt ggattaaaag agaacgtccc cggtgttgca
    18061 tttatagttc gtgaataatt tcgcaagggt tgcgattaca aaatacaaaa acagacggcc
    18121 gaaagccgtc tgtttttgta aatatcaact accaattaca acgcagcaat aatggcatcg
    18181 gccatttgtt ttgttgaggc agcgccctga ttgataacat caggtgagcc tttcattttc
    18241 agcatatcgt aagcacgaac tttgccgtcg gcaacaactt tggcaactgc cttgcgtatg
    18301 cgctgtgctt tttcgtcttc accaatgtgt tcgagcatca tcactgcaga aagtatcatg
    18361 gctatcgggt tcacgatcga caccggatag tcggcatact tgggtgctga accgtgggta
    18421 ggctcgaaca cgcacacacc gctttcggga ttgaactgtg ccgaagtggc aaaacccaga
    18481 ccaccaatca atccggcaaa tccgtcggat acaatatcgc cgaacatgtt tccggcaaca
    18541 attacgtgat aattttcagg gtttttggtc agccacatca tggttgcatc aatgttgatg
    18601 tcttcaacat aaatgttcgg atattctttc ttgctcattt cctgtgcaat ttttagcatc
    18661 atacccgaag tttcgcggat aacattgggt ttttcgctta ccgtaatttt atcgaatccg
    18721 cgtttttttg catattcaaa agccgctttc acaatgcgtg tggtatattt tctggtgaaa
    18781 atacgggtcg aaacggcgag gtcttcgcgt gggcaccatg caaagttatc gacgaatttt
    18841 ttgtgggtca tcagtccgtt gtacacaaca tcgggaggat tggtccattc aacgccgcca
    18901 tacagccctt cggtgttctg acggaaaatc atcacatcca cttcgggttc ttctatggtg
    18961 ttgttggcac cgcgacgaat gaagttgagg gggttgcctt tcagggtctg gcaaggacgc
    19021 atacagatat ccagatcgaa atgctgacgc atcgatacga tggggctgta gtaagtgtag
    19081 cctttcccct gaagttcggg agcaagctct gcagccgctt tgtctttggg ttttgaggtg
    19141 atggctccga acagaccgat tttatgttct tcgagtaatt tgagggtacg gtcgggtaga
    19201 gggttgcctt ctttgcacca gaattcccaa ccaatatcac cgtgtacata gtttgcttca
    19261 aaaccggcag cgtcgagcac gcgcagtgtc tcgttcaata ctggttttcc gataccgtcg
    19321 ccgggcatag ttacaatagt tctttttgcc atagttttca agtttaattt tgagtaaaaa
    19381 tagtatgatt tgtgtgaatg caaaactgtt gtgtagcatt tataaaccac cggatttggc
    19441 aaaaagcccg caacagatta agcgttttta tttcttacca attaccgccc ctgtatcatc
    19501 gaaatctata gccaactccc aacctgacag caactccaat cggaccttgg tgaaagtcga
    19561 aggtggctgt attccgttgg ttaactggca ataccggatt atcggttttt atgctgttat
    19621 attgtgccgt tacgagagcc aacggcacat tcaggtcgat aaaccatttc tcgctaaaag
    19681 agtaagttag cctgggaatt accgaaaggt aaatgcctgt accggttttc gttacgggaa
    19741 attccgacga tatcgatgaa tttgatttac tccacctgaa aaatggtgtt gccgagaaac
    19801 cgattgcggt tccaaatttc tcccagttct gtttcttaaa caatgttatt gtgtactcat
    19861 accgcaaaaa acaattaaaa gcctgagctg tggaggccga tagcacctgc gatattgcgc
    19921 cggtcgaatc cacgatgtaa tattccttgt tgtaattgtt attatacgcc aaacgcgata
    19981 tttccacaga atgataattc cctttatcag tgttgatttc gactgcagga gaaaaattaa
    20041 acccgtaaaa ttcccgttca aactccgtgt gtattgttcc ttcagaaggt ccctggccat
    20101 gtgtaatata atttgagttt aaattgaaat tggaatacaa tgcaatcgaa acggggtggg
    20161 ttgaattctg gctgtataca gtcatactga ttgccccaag gagaaccata aaaatgcaac
    20221 aaaatttcat agtaattatc tttagcgaac agggtaaaaa cgctgcattt atttaaaata
    20281 gtatagcagt aaaaatttta tttggattcg gtgtacatgg cattattcac cataacgttc
    20341 cgtaagtagg cgtatgtagc cctgtgttgc gcctgcggca gggctattac gcttaccttt
    20401 gactttggtt ttatttccaa aattaaaata atttttaaag ctcatcaaac ggggacctga
    20461 tattttgtaa actttttgta gatatgtgtg tgtttatctt aatggtttta ctgctcttat
    20521 ggcctaaaat cagtaaatta tcattataaa ccaaacctat ttccgtttca gcgtaaccag
    20581 tgtaatgtcg ggcggcataa caaatctccc gtgcgtagct gaagaaaaat tattggtttt
    20641 acctgaaatc ttgcaaatgt gtcaagatta tttcgtattt ttgaataaag ttgttttata
    20701 tattaaatta attgatagtc aattaattaa gaaaaattaa tataattcaa agaacaactt
    20761 attttattta tttttaaaat gcaaaaggta taattaaaca ttacagatga agaacttaca
    20821 tttactaata atgatttttt caattacacc ttctttttcg caagaaatta gcctaggtga
    20881 taatttatac acagaattta ataaaatctc agcaccctat ttttttgaaa tgagcaataa
    20941 tacgggttgg atagaagggg aagggaattt ttatattgga gatatgccaa ttttggggga
    21001 ttgtaatgtt atttatatgg cattaaaaga cacaacatta tttggattat ttgttaaaaa
    21061 accacaagct acttttatgt tcgatagcca aggtgattcc atacttgatg tttctccagg
    21121 gttttttatg cacccatttt gggttgtaaa aaacaataca aaaatttcac ctgacgataa
    21181 gaaaattatc gaagtattta ataatatata tgaaaaaacc ttgcaatcgg attattttga
    21241 taatgcagat aatcctttta gcgaatttgt taaatatcga accgatacct cattgtcgaa
    21301 ccggcatatt gcctatctct ttgagtatgt gttattatca tcaacaccca gttctgaaaa
    21361 tgataccccg gagttcgcga aattttcttt ggatttaaat ctttctttgg catcagagtg
    21421 tttgtcgctt tttcatgtga tccctgaaat tgtttatgtt tatttaggtg aagcctttat
    21481 tgcttgtgga atgactgaca aagcaattga gaattatgaa atagctttgc aactctatcc
    21541 cgattgtatc ccttgtaaag tttataattg tgttctagaa aaagattctg tgaaaaagaa
    21601 ggagaaatca aaggagttgc gaaaaaaaca tcccaaccac tggttagtaa atgaactgat
    21661 aaattagcct aaatagtacc tcatttaaat ttacccaaaa gcttttgggc ctcttataat
    21721 cgactacctt ctcttcagcg taatcagtgt aatttcgggt ggcatgccga tccgtccttc
    21781 gaaggcgata tacccgaacc cctgattaac atacaggtat ttcccgtttt cttccgttag
    21841 tcccgaccag tttggataca aatattgccc cggactccat ttgaattttc cccattcaat
    21901 accaaactgg aacccatggg tatgtcccga gagcgtaagc gccaccggaa cgggatacgt
    21961 ttttacctgc caatcccagt gcgaagggtc gtgcgacagc aatacgataa aatccttgtc
    22021 cgaaagtccg ttgagagatt tgggcaggtc gccctgtcgg atgaaattat gcccccagtt
    22081 ttcgacaccg gcaacatata ttgtgtcgct gccctttact atggctctgt gttcatttat
    22141 cagcacatcg aagccagcct gctgctcgtg gcgtaccagt tgagccagat tggcatcttt
    22201 ggcttcttgg tttttccatc gcacatagtc gccgtaatcg tgatttccga taacagagaa
    22261 cttgccaagg ggagcctgta tgtcgcggag cacaggtatc caaggcaccg cttctttagc
    22321 cagattgttc accatgtcac cggtgaaaac gaaaatatcg ggcttcaggt cttttattat
    22381 ttcaatgcct ttaattactt cgtcgcggtt gtggaagctg cctgcatgaa aatcggatat
    22441 ctgtgcaatg gttgttccgt cgaaagctgc cggcagctta tcggaataga ttgttttgcg
    22501 ggatacctta tagttgcttc tttcgaaga
//
LOCUS       DAOOQP010000037        22415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2815_length_22415_cov_5.332692, whole genome shotgun sequence.
ACCESSION   DAOOQP010000037 DAOOQP010000000
VERSION     DAOOQP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2815_length_22415_cov_5.332692"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..502)
                     /locus_tag="PLK75_05670"
     CDS             complement(98..502)
                     /locus_tag="PLK75_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOY38771.1"
                     /translation="MALQTRNKINSSFSMASMSDLVFLLLVFFMITSTMIAPNALKLL
                     LPQSNNQTQAKQNTTVSIDDLGDGVFAYAVGTSYCNFDEVERRLRVELADVQEPVVTL
                     AVDKTVPMEEVVKIMNIAVNNNYKLILRTAPE"
     gene            complement(508..1200)
                     /locus_tag="PLK75_05675"
     CDS             complement(508..1200)
                     /locus_tag="PLK75_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOY38772.1"
                     /translation="MILLQATADTLKNAANVAQATTEQGMSFLELAVKGGWIMLVLAV
                     LSVIAIYIFIDRFIAIRKASKEQASFMENIKNFIHSGKVEQALDACKMNPGTLSRMIE
                     KGIMRIGRPLNDINAAVENVGKQEIAKLEKGLPVLASIAGGAPMLGFLGTVMGMIKAF
                     YDMAHAGNNLDITLLSNGIYLAMVTTVGGLIVGIVAYFAYNVLVSKIDSLVAMLEGRT
                     TAFMDILNEPVN"
     gene            1428..2084
                     /gene="fsa"
                     /locus_tag="PLK75_05680"
     CDS             1428..2084
                     /gene="fsa"
                     /locus_tag="PLK75_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOY38773.1"
                     /translation="MKFFIDTANLDQIREAQSLGILDGVTTNPSLMAKEGIRGHVNIL
                     KHYVEICNIVKGDVSAEVISTDFEGMIREGKELASLHEQIVVKVPVIKEGIKAIKYFS
                     DNGIRTNCTLVFNQGQALLAAKAGATYVSPFVGRLDDNSTDGMELIRQIVDMFNYYEY
                     PTQVLAASIRHTMHIIKCLEYGADVATCPLNAILGLLNHPLTDIGLKKFLEDYAKMKD
                     "
     gene            2088..2708
                     /locus_tag="PLK75_05685"
     CDS             2088..2708
                     /locus_tag="PLK75_05685"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOY38774.1"
                     /translation="MALYLKIHPDNPAERLLNKAVDILRDGGVIIYPTDTVYGIGCDI
                     TKSKAVEKVSRIKGVKHDKSNFSFICYDIKHISEFTTPIENHIFKLLKRNLPGPFTFI
                     LQANNNVQRILKTNKKTVGVRVPENNIIREIVRILGNPILTTSLKNDDEIIEYTTDPE
                     LIYEDYNDLVDLVIDGGYGNNIPSTVVDCTYDEIVVVRQGLGELIV"
     gene            2816..3616
                     /locus_tag="PLK75_05690"
     CDS             2816..3616
                     /locus_tag="PLK75_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY38775.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLAAGMAVLEKKVLLVDADPQAN
                     ATSGLGFDVKNVRIGLYETLVKEEEPSNVILTTEIKGLDLLPSTIDLVGAEIEMIEIP
                     NREKIMSYILNKVKDKYDYIIIDCSPSLGLIVVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTIRIVQDHLNNRLDIEGFLITMFDSRLRLSHQIYEEVQKHFQEMVFETVIYRNVK
                     LGEAPSFGQTIIDYDVNSKGAVNYLDLAREILQKNTSNKAIEKEQITI"
     gene            3619..4503
                     /locus_tag="PLK75_05695"
     CDS             3619..4503
                     /locus_tag="PLK75_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOY38776.1"
                     /translation="MGKKNVLGRGLGALLDEKSIENAKRPYSSFDIDINEIVANPYQP
                     RENFDIELLNELAASITKLGVIQPITVKEQGNGKYQLISGERRLRAAKIAGLSKIPAY
                     VRKINDENLLEMALVENIQREDLDAIEIAISYQRLLDECNLTQEELSERVGKKRSTVA
                     NYIRLLKLPPKVQLGIRSQQISMGHARALINLKDEDTQLMIYEQILKYDFSVRKVEEI
                     ARQLSNDETNPDNAAKKIRRFTTPAEYEKLKKHLSKHFATDVDFRRYATGKGKIVIPF
                     ENDDELERIISVIDKLNV"
     gene            4506..5069
                     /locus_tag="PLK75_05700"
     CDS             4506..5069
                     /locus_tag="PLK75_05700"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOY38777.1"
                     /translation="MRIIVYIIAFIITSSVCLGQQSNTESEKAPVRKETGDSVYKSPR
                     KATIYSAVLPGLGQVYNRKIWKVPIVYAGLGGFGYFAYYNQHQFNRYKTAYINRQEGL
                     ADEFNGLLSSQGLLNEMDRFRRYRDLNILGFLAIYAIQIIDANVDAHLFRFDVSDDLT
                     FNIQPALFQTNVYAPACTGISCHIEFK"
     gene            5066..6607
                     /locus_tag="PLK75_05705"
     CDS             5066..6607
                     /locus_tag="PLK75_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOY38778.1"
                     /translation="MKKLIVIYVFFILGLNAECFCQQTDTVAVTDYGFADKLDSLLLD
                     WYKTYSNDSIYMVCVSHSNEVVTDVPDSVYRERLSTLVSPIPLDYNSLVQSYITKYIS
                     KGKWFIPDLLGKSKYYFPMFEEVLDAYQLPLELKYLTIVESALNPNAVSRAGATGIWQ
                     FMLQTARQYGLDVTSYIDERRDPYLESVAAAQYLKDLYDIYGDWHLAIASYNCGPGTI
                     NNAIRRSGGKTNYWEISKYLPYETRNYVPAFIAMNYIFSFYEEHKFFPCPSNIPVRVD
                     TVMVSQELSFGKISQILGIDIEELRLLNPQYRKDYIPARNKNYPLRLRTEKISSFIAL
                     EDSLYKKDDTVLYVAEPIQLTDNEMIADNYTPTPQPLNTEKLVYTVKSGDILGYIATW
                     YDVNVSDIKSWNGLRSNYLKIGQKIIVYKPVGVAVRYRNIDTMSFVDKQKMSNVTVAS
                     ANNQTSVTTDPKYEYYTVQSGDNPWSIANKYPGVTVEDIMKLNNISNPSSLKVGQKLK
                     IRKKA"
     gene            6730..7713
                     /locus_tag="PLK75_05710"
     CDS             6730..7713
                     /locus_tag="PLK75_05710"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOY38779.1"
                     /translation="MKTIAIFAGGNSSEREISLSTAGQIFNFIDKKLFNAYIIDVKGF
                     EWNLVEGSNRYPVDKNTFSCTVGSKEIRFDFVWNAIHGKPGENGQVQGYFDMLQIPYS
                     SCSLLTSALTFNKFMCKTYLQKQGIPMAQSVMVTAETAVDMDEIVETLGLPLFVKPNT
                     SGSSFGVSKVKKKEELIDALNIAFVEDPEVVIESFIQGTEVSCGVFKSQKNTYFLSIT
                     EIVTANEFFDYEAKYHSKLTQEITPARINDEVAKKIHKYTSAIYDALYCKGIVRVDYI
                     INGDTPYFLELNSIPGMSTESIVPKQIRTAGYNITDILTEVIEDALAKHML"
     gene            complement(7752..9029)
                     /locus_tag="PLK75_05715"
     CDS             complement(7752..9029)
                     /locus_tag="PLK75_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY38780.1"
                     /translation="MALLLKNATYLDWRNFNISVRDIWVEEGLHGKIRFFNNDDSELK
                     QMHAETLDCTDKIVTKSFAVGHHHAYSALARGMNPPKIDPKNFIEILKYIWWTLDKCL
                     DPKTIEYSALTTAIACAKAGSSFVIDHHASPFSVGNSLNIIADAFGKIGISHLLCYEI
                     SDRDGEEIAQQGLAETQKYLQKHQGLVGLHASFTVADTTLKQAVEIMEKFNSGIHIHV
                     AEDISDQEHCLNTYKKRVIERLHEAGATNSSKTILAHCLHLSDNEREIIRNSPCWVVQ
                     NMESNLNNKVGLFSGKNLGENIMLGTDGMHSDMLKSARFAYFAGQLHDDISYNQAYSR
                     FRNVHNYLSTNNFTGDGENNLVVLDYKSPTAMHSSNFPGHFLFGLEAGQVIHLISNGR
                     LILKDRILQTIDEDKVLKESRKIATKLWQKMSL"
     gene            complement(9041..11590)
                     /gene="xdh"
                     /locus_tag="PLK75_05720"
     CDS             complement(9041..11590)
                     /gene="xdh"
                     /locus_tag="PLK75_05720"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HOY38781.1"
                     /translation="MIHFILNGTVQHYTGNSENSLLHYLRIEKHITSVKDGCSGQGVC
                     GACTVEINGKARLACTVKMKNLEGAEVFTPEGFPEYVKDAIATAFVNNGAVQCGFCTP
                     GFISRVKVLLQENPNPCIDEIRKAIKPHLCRCTGYKKIEEAISNAAKVIRENKTAEIR
                     TTSGKIGVSHPKYDSYRTALGQRKFVDDIFVDGMLFAALKFSDYPKAKVLHIDIVEAN
                     KLPGVHRVFTAADIPAAHLIGLIYNDQYVMIGNGETTHYIGDVIAGVVAESEEIAREA
                     IALIKVEYEVLTPITDVFQAIQGNRVHPAKPNNFDTTSFTIGNADDALRKSKYVVEDH
                     YETQRIEHAFLEKEAAIAQPDGNGGIAVLSQSQGIYEDRRQIAMILGIPEEKVHVTLV
                     PNGGGFGGKEDMSVQGYAAMFAFLTYKPVKITLSREESIRLHPKRHPVYIDISLGADE
                     NGKLTALKLRAVGDTGAYASVGTKVMERVAGHAAGGYFIPEIDIEAKTVYTNNIPCGA
                     MRGFGANQAAFALESCIDVLCRKAGFDRWKFRWDNALVDGLRTSTGQKVFGVGIRACL
                     EAVKDDFCNAKFAGLACAIKNSGVGNGMIDESKVKIEIVSGTHIRLHHGWTEMGQGVH
                     NMALQTLCEETSVSPDIIEVIVDTEAQIKTGMTTSSRATALVGLAVINAAKALKEDLA
                     TNALADLAGKTYFGQFICDWTTKPGEIGKEPVIHYSYGYAAQVCILDDSGNISKMIAA
                     HDAGKIMNPMLFEGQIQGGVHMGLGYALCEDLPMENGYLVSDKMKDLQILRAHETPEI
                     VVKGVEVKDPVGPYGAKGIGEIGLVPTAAAVANAFYAYDGIKRTKLPLKRK"
     gene            complement(11587..12828)
                     /gene="thrC"
                     /locus_tag="PLK75_05725"
     CDS             complement(11587..12828)
                     /gene="thrC"
                     /locus_tag="PLK75_05725"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOY38782.1"
                     /translation="MKKSGNKFMYRCVDCGKEYSNESTMYLCPSCSTGNTSSAPPKGV
                     LKTIYDYTSLKNKGGFSYLKKNHFIELLPIHNISSLPQLRVGNTPLYTLNELSHKKLP
                     FRLFLKDDSQNPTFSYKDRASALVSAFAKENGYNTIVAASTGNAGSSLAGMCAMQQQK
                     AIVMVPETAPVAKLTQIIMYGAYIVPVKGSYDDAFDLSIEATQKFGWYNRNTAYNPLT
                     VEGKKTAAFEIFEQLDFNIPDRVFIPAGDGVILSGVYKGFEDLLKLEIIEKMPVIVMV
                     QSEGSDNLSRNIDSDVFTMKPSSTIADSISVDIPRNFCMAKQFISNYHGEHITVTDNK
                     IIEASKLLAENTGIFAEPAAATAFAGMLAYTDDNLLAENSVNVVMLTGSGLKDLKSVE
                     RILNIPESIDPDIRKLKHLFK"
     gene            complement(12939..14411)
                     /locus_tag="PLK75_05730"
     CDS             complement(12939..14411)
                     /locus_tag="PLK75_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOY38783.1"
                     /translation="MIQLIDKVSNEKALQNAVKRFKDRGIVLPTFAQQQNPELIPSKV
                     KNELKNIGLWEINPLNLFRITWKNEPKEKGGLYGKVNYIELPKQITGVNARIVLLIGK
                     WFPTGAHKVGAAYGCLAPRITTGGFDPTFHKAVWPSTGNYCRGGAFDSKLMGTESVAI
                     LPEEMSQERFNWLRNEIGSEVIATPGCESNVKEIYDKCWEIRRTRPDCIIFNQFDEFG
                     NAAWHYNITGKAIEEVYNDVKTPKSKLSAYISATGSAGTIAAGDYLRTIAPMIKVVAS
                     EALQCPTLLMNGFGGHRIEGIGDKHVPWIHNVKNTDVVTAIDDEDCMRLLRLFNEKEG
                     QKYLREAGVDKNVINNLHLIGISGIGNLLSAIKTARHFEMNENDILVTIATDSADMYQ
                     SRIAELNSDRGDYNQKQAIKDLEKCLYGQRTDFMKELTYSDRKTIHNLKYYTWIEQQG
                     KELEDLNQLWYDEDIWDKLFHQINVWDELITDFNRRTGLL"
     gene            complement(14506..17772)
                     /gene="ygfK"
                     /locus_tag="PLK75_05735"
     CDS             complement(14506..17772)
                     /gene="ygfK"
                     /locus_tag="PLK75_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOY38784.1"
                     /translation="MSGNFNPIPLKKLLQIILNELHTSGSVFGIPSELFFIPDSTNTY
                     KTTQFNTLIDTPVGVAAGPHSQMAQNIIAAWLMGARYIELKTIQTLDELEVPKPCIDM
                     QDEGYNCEWSQELRINQSFNEYLNAWIIIHILHQELGFSNNVNTVFNMSVGYNLEGIM
                     QANVQWFFEKMNDCTVELTEKLESIKDYYPEITALNIPSKISDNITLSTMHGCPANEI
                     ESIAFYLLTEKKLHTYIKLNPTLLGPDQLRQILNHTSKFKTQVPDIAFEHDLKYPDAL
                     KIIRSLQKTATDHRLCFGVKLTNTLESVNNKNIFSDNVEMMYMSGRALHPVSIALAKK
                     LNDDLQGEIPFSFSAGVDAFNISDLISCGFQTITVCSDLLKPGGYMRLNQYFEELSKN
                     FSDKGVSNIKDFILATAGVDDITQAKSINLSTYFNETIQSSRYRREYITTPSIKTQRQ
                     LGKFDCISAPCKDSCATNQDIPNYMYHTSQSDFKSAYRVILQTNPFPAITGMICDHLC
                     QNKCTRINYDSALHIREVKRFISEQNEVQRDVYGAKNDVKVAVVGAGPSGLSCAYYLA
                     LAGFQVDVFEEKSQAGGMVQFAVPGFRLSDEAIRSDFKRVADLGVNIHYNSRIDTEKF
                     ELLRKSYTYIYLAPGAQLSANFTIEGYNSRGVIDSLGFLFKARKREDTGIGKNIVIVG
                     GGNTAMDAARTAWRLVGKDGKVTIVYRRTINEMPADQGEIKAVIEEGIEIIELVAPEK
                     VISYNGRVSALLCSRMELSGKDASGRPKPVKIPNSEFEISCDTIIPALGQMTNIDFVQ
                     NDMLAPEASGYMTKLENIFIGGDAKRNAATAIIAISDGRRTAEEIIHKAAPWLEIPHL
                     HNIKNLTKKDLMLKRARRISAIKLWEAPPETRQSFAPVSRTPDKQSIVTESGRCLYCD
                     EICNICTTVCPNFANRCYEIAPMEFMLKKAQISENGKIELIDDVLFSIQQKYQIINIA
                     NFCNECGNCNTFCPTSSAPYIEKPKIWLTAESFASATKGYYFDSQTNALLFKRDEQLS
                     TLTLKNGVYIFETDICIAHFNATNFSLEEIALKIPSQEALHFYDAAIMMILYNVVKNL
                     "
     gene            18067..20454
                     /locus_tag="PLK75_05740"
     CDS             18067..20454
                     /locus_tag="PLK75_05740"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38785.1"
                     /translation="MNIKLALLVNLIMTLSGSVFGQAVKISVTDTHRDPLVGATIQMI
                     NVVDSTQFFATTNQYGSGIINDVEQGLYTIKISYIGYKPIEKNISIKPDKREFSFQLS
                     EDAIALNEVTVEARKPLIRHEDDKMIIDPEPLALVTTNTLEVLESTPGIYVDQDGGIY
                     LNGASPAAIYINGREQKMSSQDIAMILRSLPPGSIQSIEVLRTPSAKYDASSSGGIVN
                     VILKKGVKIGRFISIRAGMNQGKYGNRFAGFNYNDGSDLTSYYVNGNYTFQDMIEDLN
                     SERMLNADTLLNQNAQTRRNSHQAYLGYGINYYPNDKITYNYDGRINASFPYSASDNY
                     NIVVNGSEEQLSESETMTNSWNDAFNVQQEFGMVYKIDTISSNIDTKLSYNFNHNNAF
                     QNYSSNYYFPFEASVLGEGNHLQNRHFVQFQSDLTYQFPHKIKLESGVKTSFQHYASD
                     NEYFFNVNQSMIADPIRTNAFFYNENINAAYLQLSKTFWKHLQLKTGVRIEHTFMNGV
                     QTVPTDTSFLLNRADWFPYVYLSRRVLEVKGFEARVFLIYRKTINRPGYQNLSPNIKY
                     VDDFLYETGNPELKPEFTDNFELNISVEDMPLFAVGRSYTRDIISNVLYTDDNYPNIV
                     VNTYDNLSNSTETYLRGMAGIPPGGRYYFAVGAQYNLNEYEGFYDNTPFSYKRGSWRL
                     FTFHSLKLFKNTRITLSGFMMINGMQNFYELKNFGQVNFGITQRFFDDRLTISINGRD
                     ILRTMVTQFEMHQGSISVAGSRYSDNQRFGIQIRYNFGLKKPEKKPNEIKYDIDE"
     gene            20848..>22415
                     /locus_tag="PLK75_05745"
     CDS             20848..>22415
                     /locus_tag="PLK75_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOY38786.1"
                     /translation="MNTIRLFQDFTQKKSIISRFFFVFMLTLLLQLPANLFADNTVTI
                     GSGTNTGYLVPANTYYNYSYTQQIVLQSEIATAGQITKIRFYMVGGISLSNSNNWTIY
                     LGHTTKTSFSSTTDWVPLASMTQVFSGTIAATPVAGWYEIVLTGTFIYNNTDNLVVAV
                     DENATGLNGSTNYVRIWTTPVANRAMHYRSDGTNPDPASPPTATGRLSYMNQMQLDIG
                     APPTCLQPSGLSTTNITSSSATVNWTASTSNPSGGYDIYYNTSSTAPTAGTTPLESVA
                     AGVTTYNMSSLSAATTYYVWVRANCGGGDLSSWAGYTSFTTACTNAALTVNEGFNTSG
                     TSVFPTCWSQQYVTGTSALSFLTSGSYPTVSAPYEGTRMVYWNSFNYSNGQQTRLVST
                     PITTTGTASVDVEFRWYHGTDGGSSSFQTEGVQVQYSTDGSTWVNAGSFIKRYDATAG
                     WSLKTITLPVGAGNQPTIYVGFLFTSNYGYNCFLDAASIKPSPSCLTPTALMSSPTST
                     TSGTVSWTASTSNPS"
BASE COUNT         6486 a         4539 c         4645 g         6745 t
ORIGIN      
        1 atggtaaaac ttaatagcaa taaaagtatt attgctgctg catgtaccag caaagtaacg
       61 ataatacctt ctttatgttt gttaaaaaaa ctcatgtcta ttcaggagct gttcttaaaa
      121 taagtttata gttattattt acagcaatat tcattatttt aaccacttct tccattggaa
      181 cggttttatc taccgcaagg gtaaccacag gttcctgtac atctgccagt tcaaccctta
      241 gccttcgctc tacctcgtcg aaattgcaat aactcgtgcc gacagcatat gcaaaaacac
      301 catcgccaag gtcatcaatc gaaacggttg tattctgctt tgcctgggtt tggttgttgc
      361 tctgaggtag taaaagtttt aacgcatttg gtgctatcat tgtggaagtg atcatgaaaa
      421 atacaagcaa caggaacacc aaatccgaca tggaagccat gctgaaggaa gaatttattt
      481 tatttcgggt ttgcagtgcc atttttttta gtttacgggt tcattaagaa tgtccataaa
      541 tgccgtagta cgtccttcga gcatagcaac cagtgaatca atttttgaaa caagtacgtt
      601 atatgcaaaa taagcaacaa ttccaacgat aagacctcca acagtagtta ccatagctag
      661 atagattcca ttcgaaagaa gtgttatatc cagattattt ccagcgtgag ccatatcgta
      721 gaaagctttg atcatcccca ttaccgtgcc gagaaaccca agcatcggag ctcctcctgc
      781 aatggaagcc agcacaggaa gacctttttc gagctttgcg atttcctgct tccccacatt
      841 ttcgacagcg gcattaatat cgttcaaagg tcttccgata cgcataattc ccttttcaat
      901 cattctcgat aaagttcccg gattcatttt acatgcatct aatgcttgtt ctacttttcc
      961 gctatggatg aaattcttga tattttccat aaatgatgct tgctctttcg atgctttgcg
     1021 gattgcaatg aatcggtcga tgaaaatata gattgcaatt accgaaagaa ccgccagaac
     1081 taacattatc cagccgcctt taacggctaa ttcaagaaac gacataccct gctctgttgt
     1141 ggcctgagca acattggcag cgttttttaa tgtgtcggct gttgcctgaa gaagaatcat
     1201 aataggttaa gtttaaatca aaaacgaaat tagaaactct atatttcttt taggctgtgt
     1261 tattactgtt tttatcaaca tggttttgtt gagaatgccg ttggtttaca aactcataac
     1321 ggtgtgttac gcgaaattat tttataaaag gctgcaattg ttattttctt taaatacggc
     1381 ttgcggaatt tttaactact tttgacggtc aaatcaatac acgcaatatg aaattcttta
     1441 tcgacacagc aaaccttgac cagatacgtg aagcacagtc gctgggcatc ctcgatggtg
     1501 ttactacaaa tccgtcgctc atggctaagg agggaattcg ggggcatgta aacatcctga
     1561 aacactatgt tgaaatatgt aacattgtta agggagacgt gagcgctgag gttataagca
     1621 ccgatttcga aggaatgata agggaaggta aagagcttgc atcgctccat gagcagattg
     1681 ttgttaaagt gccggtgata aaagaaggga ttaaagccat taagtatttc agcgataatg
     1741 gtatccgtac caattgcaca ctggtattca atcagggaca ggctcttctc gcagcaaaag
     1801 ccggagctac atatgtttct ccttttgtgg gcagactcga tgataattct accgacggaa
     1861 tggagctaat tcgtcagatt gttgatatgt tcaactatta tgaataccca acacaggttc
     1921 ttgcagcctc aatcaggcat accatgcaca taataaaatg ccttgagtac ggtgccgacg
     1981 tggcaacatg tccgttgaat gcaattcttg ggttattgaa ccatccgctt accgacatag
     2041 ggctgaagaa attcttggaa gactatgcaa aaatgaaaga ttgatttgtg gctttatatt
     2101 taaaaataca ccccgacaac cctgcagaac gacttctaaa caaagcggtt gatatattgc
     2161 gtgatggcgg tgtaattatt taccctaccg atacagtata cggaatcgga tgcgatatca
     2221 cgaaaagcaa ggctgttgag aaggtttcga ggattaaagg agtgaagcac gataaatcaa
     2281 atttctcttt tatttgttac gatataaaac atatttcgga gtttacaaca cctatcgaga
     2341 atcatatatt caaattgctg aaacgcaacc tgcctggccc tttcactttt atactgcaag
     2401 caaataacaa tgtgcaacgc attcttaaaa caaacaaaaa aaccgtgggt gtgagggttc
     2461 cagagaataa tattattcgg gaaattgtaa gaatactggg taatccgata ttaaccactt
     2521 cgctgaaaaa cgatgatgaa attattgaat atacaaccga cccggagtta atttacgaag
     2581 attataacga tctggttgat ttggtaattg atggcgggta tggaaataat atcccgtcaa
     2641 cagttgttga ttgcacttat gatgagatag ttgtggtgcg gcagggattg ggtgaattaa
     2701 ttgtttgata ggttcagtgc aaccggatat taacataaat gttaaaaatt ttgactcctc
     2761 gaatgcacat tcattgaacc gataattgta tttttggcaa attttaaaat gtaatatggg
     2821 aaaagtaatt gcaatagcca accaaaaagg gggtgtcgga aagactacta cggcaataaa
     2881 ccttgcagca ggtatggctg tgctcgaaaa aaaagtgtta ctggtggatg ccgatccgca
     2941 ggcaaatgcg acatccgggc tgggcttcga tgttaaaaac gtaaggattg gattatatga
     3001 gactctggta aaagaagaag aaccatcgaa tgtaattctt actaccgaaa tcaaagggct
     3061 cgatttattg ccaagcacca tcgatctggt tggtgccgaa atcgagatga ttgaaatacc
     3121 gaaccgggag aaaataatga gctatatatt aaataaggta aaagacaagt acgattacat
     3181 aattatcgat tgctcaccgt cgcttggact gatcgttgtc aatgcactta cggcagctga
     3241 ttcggtgatt attccggtgc agtgcgagta ttttgctctt gaaggacttg gtaaattgct
     3301 gaatacaatt cgcattgttc aggatcattt gaataaccgg ctcgatatag aaggttttct
     3361 aattacaatg ttcgattctc gccttcgttt gtcgcatcag atttacgaag aagttcagaa
     3421 acattttcag gaaatggttt ttgaaaccgt aatttataga aatgttaaac tgggagaagc
     3481 tccgagtttc ggacagacaa ttatcgatta tgatgtaaac tcaaaaggtg ctgtaaacta
     3541 tttagacctt gcaagggaaa tactacagaa aaacacaagc aataaagcaa tagagaaaga
     3601 gcaaattact atttaactat ggggaaaaag aatgttctgg gaagaggatt gggtgcactg
     3661 ctcgatgaaa agtcgattga aaatgcaaag cgaccttaca gctctttcga tatagatata
     3721 aatgagattg ttgccaatcc gtatcaaccg cgtgaaaact ttgacataga gctgctcaac
     3781 gaactggcag catcaattac caaactgggt gttattcaac ccatcactgt taaggagcag
     3841 ggaaatggca aatatcagct aatttcgggt gagcgtcgtt tacgggcagc taagattgcc
     3901 ggattatcga aaatccctgc ttatgtgcgt aagatcaatg atgaaaacct ccttgaaatg
     3961 gcactggtcg agaatattca gcgcgaagac cttgatgcca tcgaaatagc tatttcttat
     4021 caacgattgc tagatgagtg caatctcact caggaagaac tgagcgaaag ggtgggtaaa
     4081 aaacgctcta cggtagccaa ttatatcagg ctgttgaaac ttcccccaaa agtacaactt
     4141 gggataagaa gccagcagat atccatgggg catgcgcgcg ccctgatcaa cctgaaagat
     4201 gaagataccc agttaatgat ttatgaacaa atactaaaat acgatttttc agttcgcaaa
     4261 gttgaagaaa ttgctcggca acttagcaat gatgaaacca accccgacaa tgctgctaaa
     4321 aagattcgac ggtttaccac cccagccgaa tatgaaaaac tgaaaaagca cctttcgaag
     4381 cactttgcaa cagatgtcga tttccgtcgc tacgctaccg gtaaaggaaa aattgttata
     4441 cctttcgaaa acgacgacga gctggaaagg attatttcag ttattgataa actaaacgtt
     4501 tgatattgag gataatagta tatattatag cctttattat tacttcttcg gtgtgtttgg
     4561 gtcagcaaag caacactgaa tcagaaaaag cacctgttag aaaagaaacc ggcgacagtg
     4621 tttataaatc accccgcaaa gccacaattt attcagcagt gcttcccggt ctgggacagg
     4681 tgtataaccg aaaaatatgg aaagttccta ttgtgtatgc agggctgggt ggttttggtt
     4741 attttgcata ctataatcaa catcagttta accgctataa aactgcatac atcaaccgtc
     4801 aggaaggtct tgcagatgag ttcaacggat tactttcgag ccaggggttg cttaacgaaa
     4861 tggatcgctt caggcgttac agagacctga atattctggg cttccttgca atttatgcca
     4921 ttcagattat cgacgccaac gtcgatgctc atttgttccg gtttgatgtt tccgatgacc
     4981 ttacttttaa catccaaccc gcgttatttc aaacgaatgt ttatgctcct gcctgcacag
     5041 gtattagctg ccatatcgaa ttcaaatgaa aaagttaata gtaatatatg tattttttat
     5101 actcggtttg aatgctgagt gtttctgtca gcagacagat actgttgccg taactgatta
     5161 tggctttgcc gacaaactcg acagcttact cctcgactgg tataaaacct attcaaatga
     5221 tagtatttat atggtctgtg ttagtcactc caatgaggtt gttactgatg ttccggacag
     5281 cgtttacaga gagaggcttt cgacccttgt atctccaatc cccctcgatt acaattcact
     5341 tgttcagtcg tatataacca aatacatcag taagggcaag tggtttatac ctgatttgct
     5401 cggtaagtcg aaatattatt ttccgatgtt tgaagaagtg cttgatgcat atcagcttcc
     5461 gctggagttg aagtacctga cgatagtgga gtcagcactg aatcccaatg cagtgtcgcg
     5521 tgcaggagca actggcattt ggcagtttat gctgcaaact gcacgacaat atggacttga
     5581 tgtaacttcg tatattgatg agaggcgcga tccgtatttg gaatcggtag ccgcagccca
     5641 gtacctgaaa gacctgtacg atatttacgg cgactggcat cttgccatcg catcctacaa
     5701 ttgtggaccg ggaactataa acaatgccat tcgccgatcg ggtggtaaaa caaattactg
     5761 ggaaatttcg aagtacctcc catatgagac tcgtaactat gtaccagctt ttattgccat
     5821 gaattatata ttcagcttct acgaagaaca taagtttttt ccttgcccgt cgaatatccc
     5881 ggtaagggtc gatacagtga tggtatcgca ggagctgagt tttggtaaaa tttcgcagat
     5941 acttggaatc gacatagaag aattaaggct gcttaatcca caataccgta aggattatat
     6001 tcctgcccgc aataaaaact atccccttcg ccttcgcacc gaaaaaatca gttcttttat
     6061 agccctcgaa gactcattgt ataaaaagga cgatactgta ctctatgttg ctgagcccat
     6121 ccaattgacc gataacgaaa tgattgcgga taactatacc cctacgcctc agccattaaa
     6181 taccgaaaaa ctggtatata cggtaaaatc tggtgacata ttaggctaca ttgccacatg
     6241 gtacgacgtt aatgtttcgg atattaagtc gtggaacggc ttgagaagca attacctgaa
     6301 aatagggcag aagatcattg tttacaaacc ggttggtgtt gccgttcgtt acagaaatat
     6361 cgataccatg agttttgttg ataagcaaaa aatgagcaat gtaacggttg cttctgccaa
     6421 caatcagaca tcagttacaa ccgaccctaa atacgaatat tacacagttc agtcgggcga
     6481 taatccatgg agcatcgcaa acaagtatcc aggggttacg gttgaagata tcatgaaact
     6541 gaataatatt tctaacccgt cttcactaaa ggtcggacaa aaacttaaaa tcagaaagaa
     6601 agcctgataa ttgtatttta tgaaaaggca gttgttcatg ttttttttaa aatcaatgaa
     6661 taattccggt taattttcag tttttttaaa gtttaattaa aagctcaaat gcaaactgat
     6721 tattaagcta tgaaaacaat tgccattttt gccggaggaa attcttcaga aagagagatt
     6781 agcttatcga ctgccggaca gatttttaat tttatcgata aaaaactgtt caatgcttat
     6841 attatagatg taaagggttt tgaatggaat cttgtagaag gcagcaacag atatcctgtc
     6901 gataagaata ctttttcatg cactgtcgga agcaaagaaa ttcgtttcga ttttgtatgg
     6961 aacgctattc atggaaaacc cggagaaaac ggacaggtgc agggatattt cgatatgcta
     7021 caaattccat attcttcgtg cagtttgctt acatccgcac tgacattcaa taaattcatg
     7081 tgtaaaacgt atttacagaa gcagggaatt ccgatggctc aatcagttat ggttactgct
     7141 gaaacagccg ttgacatgga tgagattgtg gagaccctgg gattgcctct ttttgttaaa
     7201 cccaatactt cagggtccag ttttggagta agcaaggtaa agaaaaagga agaactgatt
     7261 gatgcgttga acattgcctt cgttgaagat cctgaagtgg tgattgaatc gttcattcag
     7321 ggaaccgagg tttcgtgcgg tgtttttaaa tctcaaaaaa atacctattt tttatctatt
     7381 acggagattg ttacggcaaa cgaatttttc gattacgaag caaaatatca ttcaaaactg
     7441 actcaggaaa ttacacccgc tcgaataaac gacgaagttg caaagaagat acataaatat
     7501 acatctgcaa tctacgatgc gttgtattgc aaaggtattg ttagggtcga ttacattatt
     7561 aacggagata caccctattt tctggagcta aattcaatcc cggggatgag caccgaaagc
     7621 attgtgccaa aacagatcag gactgccgga tacaatatta ccgacatact tactgaggta
     7681 attgaagatg cattggcaaa acatatgctt tgaaaatagc atcatcccga tgtaaaaggg
     7741 aataatattg gtcacaaact cattttttgc cataacttag ttgcaatctt acgcgattct
     7801 tttagcactt tgtcttcgtc tatagtttgc aaaatacggt ctttaagtat cagcctgcca
     7861 ttcgatatta aatgtataac ctgcccggct tccagaccaa acaaaaaatg cccgggaaaa
     7921 ttagagctat gcattgctgt aggcgattta taatcaagca ctaccaggtt gttttcacca
     7981 tcgccggtaa aattgttagt actcagataa ttatgtacat tcctgaaccg gctgtatgcc
     8041 tgattatagc tgatgtcatc atgtaactgg cctgcaaaat aggcaaaacg tgcacttttc
     8101 agcatatcgc tatgcatacc atcggttccc agcatgatat tttcacccag attttttcct
     8161 gaaaacaaac caactttatt gttcagattg ctttccatat tctgaactac ccagcatggt
     8221 gaattcctga tgatttctct ttcattatcg cttaggtgca aacaatgagc aagaattgtc
     8281 tttgaactat ttgttgcccc ggcttcatga agacgctcta taaccctttt tttatacgtg
     8341 ttgaggcaat gctcctggtc ggaaatgtct tcagccacat gaatgtgtat accggaattg
     8401 aacttttcca tgatttcaac tgcctgtttc agtgtggtat ctgcaacggt gaacgacgca
     8461 tgcagcccga ccaggccctg atgcttctgc aggtactttt gggtttcggc aaggccttgt
     8521 tgtgcaatct cttcaccgtc gcggtccgaa atctcgtagc acagtaaatg agaaattcct
     8581 attttgccga atgcatctgc aatgatgttc agggagtttc ctactgaaaa tggcgaagca
     8641 tggtgatcaa ttacaaagct cgaaccggct tttgcacagg caattgctgt tgtgagcgca
     8701 ctatattcaa tagtttttgg atcaaggcat ttgtcgagtg tccaccagat gtatttcaga
     8761 atttcgataa aattcttcgg gtcgattttt ggcggattca tgcctcttgc aagagctgaa
     8821 tatgcatgat gatgtccaac ggcaaacgac ttggttacta ttttatctgt gcagtccagc
     8881 gtttcggcat gcatttgttt caactcagaa tcgtcattat tgaagaacct gatttttccg
     8941 tgcaatcctt cttcaaccca tatgtcgcga accgatatat taaaatttct ccagtcgaga
     9001 tatgtggcgt ttttaagtaa tagagccata gtgcattgtt ttattttctt ttcaggggga
     9061 gtttggttct tttaattcca tcgtaagcgt agaaagcatt ggctacagca gcagcagtgg
     9121 gcaccagacc gatttccccg atgccttttg caccgtatgg acccacgggg tctttcactt
     9181 caacaccttt cactacaatt tcgggggttt catgggctcg aagtatttgc aggtctttca
     9241 ttttgtcgct gaccagatat ccgttttcca ttggcaaatc ttcgcataaa gcataaccca
     9301 gccccatgtg tacacccccc tgaatctgac cttcgaagag catagggttc atgattttac
     9361 cggcatcgtg agcagcaatc attttagaaa tgtttccaga atcatcaaga atacacacct
     9421 gggctgcata accgtaggaa taatgaatca ccggttcttt tcctatttcg ccgggcttgg
     9481 tagtccaatc gcaaataaac tgtccgaaat aagttttgcc tgccaaatcg gcaagggcat
     9541 ttgtcgctag atcttccttt aaagctttgg ctgcgtttat tacagccagt ccaacaagtg
     9601 cggttgctct cgatgatgtg gtcatgcctg tcttgatctg tgcctcagta tcaacaatca
     9661 cctcaatgat atcgggagaa acggaggttt cttcgcataa ggtttgcaga gccatgttgt
     9721 gtactccctg acccatttcg gtccaaccat ggtgcagacg tatatgagtc cctgatacga
     9781 tttcgatttt tacctttgat tcgtcaatca ttccgttccc gacaccggaa ttctttattg
     9841 cacatgccaa tcctgcaaat ttggcattac agaagtcatc tttaaccgct tcaaggcatg
     9901 ctctaattcc aacgccaaac accttttgtc ctgttgaggt tctaagtccg tcgacaagtg
     9961 cattatccca gcgaaatttc catcggtcga atccggcttt tcggcataaa acatcaatac
    10021 agctttcgag ggcaaaggct gcctggtttg ctccgaatcc acgcatggca ccacagggaa
    10081 tattgttggt atacacagtt tttgcctcga tatcgatttc agggataaaa tagccccccg
    10141 ctgcatgtcc ggcaacgcgt tccatcacct tggtccccac cgaagcatac gccccggtgt
    10201 caccgacagc ccgaagtttt aatgctgtga gcttcccgtt ttcatctgca ccaagggaga
    10261 tgtcgatgta aaccgggtgc cgtttcgggt gcaagcgaat cgattcttcg cggctaaggg
    10321 taattttaac gggtttgtag gtcaaaaatg caaacatggc tgcatagccc tgcacactca
    10381 tatcttcttt cccgccaaag ccgccaccgt tgggaaccag tgtaacatgc actttctctt
    10441 cggggatccc caaaatcatg gcaatctgcc tgcggtcctc gtaaattccc tgactttgac
    10501 tgagcactgc tatacccccg ttcccatctg gctgggcaat agcagcttct ttttcgagga
    10561 aagcatgctc aatacgctgg gtctcgtaat gatcctcaac tacatatttc gacttccgga
    10621 gggcatcatc tgcattcccg attgtaaagg aggttgtgtc gaaattattc ggtttcgcgg
    10681 gatgcacccg attgccttga attgcctgaa agacatcggt tatcggagta agcacttcgt
    10741 attctacttt aatcagggca atggcttctc ttgcaatttc ttccgattcg gcaacaacac
    10801 cagctatcac atctccgata taatgggttg tttcaccgtt gccaatcatt acatactggt
    10861 cgttgtaaat caacccaatc aagtgagcag caggaatatc ggctgccgta aaaacgcgat
    10921 ggacgccagg cagtttattt gcttcaacga tatcaatgtg taagactttt gctttcggat
    10981 aatcgctgaa tttgagcgct gcaaacaaca tcccgtcaac aaaaatatca tcaacaaact
    11041 tacgttgccc caaggctgtg cggtatgaat catatttggg gtgcgacaca cctattttcc
    11101 cggatgtggt acgaatttct gcggttttat tttcacgtat aactttagca gcattactta
    11161 tggcttcctc aattttctta taccccgtac agcggcacaa gtggggttta atagctttgc
    11221 ggatttcatc gatacaggga ttcgggtttt cctgaagaag tacctttaca cggctaataa
    11281 aaccgggagt acaaaaacca cattgcactg ctccgttgtt cacaaaagct gttgcaatgg
    11341 catctttaac atattcgggg aagccttcgg gtgtaaacac ttcagcgcct tcaagatttt
    11401 tcattttaac agtacaggca agccgcgctt ttccgttgat ttctaccgta caggcaccgc
    11461 atactccctg ccccgagcag ccgtctttta ccgaagtgat atgtttttcg attcgcaaat
    11521 agtgcaatag cgaattttcg gaattgcccg tataatgttg tacggtacca tttaaaatga
    11581 aatgtatcat ttgaaaagat gtttcagttt tctaatatcc ggatcaatcg attcaggtat
    11641 attcagaatt ctttcgactg atttcaggtc tttgagtcca ctgcctgtaa gcattacaac
    11701 attcacggag ttttcggcca gtaagttatc atcggtgtaa gctaacattc ctgcaaaagc
    11761 tgtggcagca gcgggttctg cgaaaatgcc ggtattttca gccagcaatt tcgaggcttc
    11821 aattatttta ttatctgtta ctgtgatatg ttccccatga taattgctga taaattgttt
    11881 tgccatacaa aaatttctgg gaatgtccac cgaaatcgaa tcggcaatcg tgctactggg
    11941 tttcattgtg aagacatctg agtcgatgtt tctcgacaaa ttatcgctgc cttccgactg
    12001 caccattact ataaccggca ttttctcgat gatttcgagc tttagtaagt cttcgaaccc
    12061 tttatatact cccgataaaa tcactccatc accggcagga ataaaaaccc tatcaggaat
    12121 gttgaaatcc aactgttcaa aaatttcgaa tgcagcagtt tttttaccct ccacggtaag
    12181 cgggttatat gcagtattcc ggttatacca tccaaatttc tgtgttgctt caatacttaa
    12241 atcgaatgca tcgtcgtagc tgccttttac aggcactata tatgctccgt acatgattat
    12301 ctgtgtcagt ttagcaacag gagcagtttc aggaaccata acaatggctt tctgctgttg
    12361 catggcacac atccctgcca acgatgaccc tgcgttacca gttgatgctg caacaatagt
    12421 gttgtatcca ttttcttttg caaatgccga tacaagtgcc gaagccctgt ctttatacga
    12481 aaaagtcggg ttctgggagt cgtcttttaa aaacaaccgg aaagggagct ttttgtggct
    12541 gagttcgttc agtgtgtata acggtgtatt tccaacccgt aattgaggta agctcgaaat
    12601 attgtgtatt ggaagcagtt cgataaaatg attttttttg agatagctga aaccaccctt
    12661 gtttttcaga gaggtatagt cataaattgt tttcaacaca ccttttggtg gagcagaaga
    12721 tgtgttaccg gtgctacacg aagggcacag gtacatagta ctttcgttgc tgtattcttt
    12781 tccgcaatca acacaacggt acatgaattt atttccggat tttttcaaaa caacacgatt
    12841 tatatatgtg ctaaattaat gaattatttg caaaaaaagc gtagttgatg ccctacgctt
    12901 tcgataaaat tattctttgt tatcgttact tttaattatt atagtaagcc ggttcttctg
    12961 ttaaaatcag taatcagctc atcccaaaca ttaatctgat ggaaaagttt atcccagata
    13021 tcctcgtcgt accacaactg attgaggtct tccagctctt ttccctgctg ctcgatccat
    13081 gtatagtatt ttaagttgtg gatagttttg cggtcggagt aagtgagttc tttcataaaa
    13141 tcggttcttt gaccgtagag gcacttctcc agatctttta tcgcttgctt ttgattgtag
    13201 tcacctctgt ctgaattaag ttcagcaata cgcgattgat acatatcggc agaatcggtt
    13261 gcaatagtta ctaagatatc gttttcattc atttcgaaat gacgggctgt tttaattgca
    13321 gaaagcaaat taccaatacc tgaaattccg atgagatgaa ggttgtttat tacatttttg
    13381 tcaacaccgg cttctctaag atatttctgc ccttcttttt cgttaaaaag ccttagcagg
    13441 cgcatacaat cttcgtcgtc gatggcagta accacatcgg tatttttcac attgtggatc
    13501 catggaacgt gcttatcacc aatgccttcg atacggtgcc cgccaaaacc gttcatcagc
    13561 agggtggggc actgcagtgc ttccgatgca actactttta tcatcggggc aattgtacga
    13621 agataatcac ctgcagcaat ggtgccggct gagccggtag ctgatatata ggctgacagt
    13681 ttgcttttcg gggtttttac atcgttgtac acttcttcta tggctttgcc ggtgatgtta
    13741 tagtgccacg cagcattgcc gaactcatca aactgattga atattatgca atcgggccgg
    13801 gttcttctta tttcccagca tttatcgtag atttccttaa cattcgactc gcatccgggt
    13861 gtggcaataa cttcagagcc aatctcgtta cgcagccagt tgaagcgttc ctgactcatt
    13921 tcttccggta aaatagctac cgattcggtc cccatcagct tggaatcgaa agcaccgcca
    13981 cggcagtaat tcccggtgga aggccacacc gctttatgaa aggttgggtc gaaaccacct
    14041 gtagtgattc tgggtgcaag gcaaccatag gcagctccga ctttatgtgc tcctgtagga
    14101 aaccatttcc caatcagcag tacaattctt gcattaaccc cggtaatttg tttgggtaat
    14161 tcaatgtaat tcactttgcc atatagccca cctttttctt ttggctcgtt tttccatgtt
    14221 attctaaaca ggttgagagg gttgatttcc cataagccaa tattctttaa ctcatttttt
    14281 actttggagg gtataagctc agggttctgc tgctgagcaa atgttggaag cactattcct
    14341 ctgtctttga aacgttttac ggcattttgc aatgcttttt cgttactgac tttatcaatc
    14401 agttgaatca tattactgta gatttaatga aaattgccga aatttataaa taatatctga
    14461 tgaatatccg aaaaagttca tgcgggtttt attggatcaa ttaaattaca aattttttac
    14521 aacattgtaa agaatcatca taatcgcagc atcataaaag tgcaaggcct cctgtgatgg
    14581 gatttttaaa gcgatttctt caagggagaa attagtagcg ttgaaatgtg caatacatat
    14641 atcggtctca aaaatatata ctccgttttt taaagtcaat gtagaaagtt gctcatctct
    14701 tttgaaaagt agggcatttg tttggctgtc gaaataatac cctttggtgg cactggcgaa
    14761 cgactcagct gttagccaaa ttttaggttt ttcgatatac ggtgcagacg aagttgggca
    14821 gaatgtgttg caattgccac attcattgca aaaatttgca atgtttataa tttggtattt
    14881 ttgttgaatg gaaaacaaaa cgtcatcaat caattcaatt ttcccgtttt ccgaaatttg
    14941 ggcttttttc aacatgaatt ccattggcgc aatttcgtaa caacggtttg cgaaattggg
    15001 gcaaacggta gtgcatatat tgcaaatttc atcacagtac aggcagcgac ctgattcagt
    15061 cactatgctt tgcttatcgg gtgttctgct taccggagca aacgactgcc ttgtttccgg
    15121 aggtgcttcc cacagtttga ttgctgaaat acgtcttgca cgttttagca tcagatcttt
    15181 ctttgtaagg tttttaatgt tgtgcagatg cggtatttcg agccaagggg ctgctttgtg
    15241 tataatttcc tctgcagtgc gacgtccgtc agaaatagca ataatggctg ttgcagcatt
    15301 gcgctttgca tcgccaccga taaaaatgtt ttcgagtttt gtcatatatc ctgatgcttc
    15361 aggtgctagc atatcgtttt gtacaaaatc aatatttgtc atctgaccca gtgctgggat
    15421 gattgtatcg caagaaattt cgaattctga atttggaatt tttactggtt tggggcgacc
    15481 agaggcatct ttccccgaaa gctccattct gctgcagagc aaggcgctta ctcttccatt
    15541 atacgaaatc actttttcgg gggctaccag ctctattatc tctattcctt cttcgatgac
    15601 ggctttaatt tcgccctgat cggcaggcat ttcgtttatg gttcgccggt aaacaatagt
    15661 tacttttcca tctttcccaa caagccgcca tgcagtccgt gcagcgtcca tggcagtgtt
    15721 tccgccccca acgataacga tatttttacc aataccggta tcttctcttt ttcttgcctt
    15781 gaacagaaat ccaagtgagt cgataacacc tctggaatta tacccttcaa tggtaaaatt
    15841 tgctgaaagc tgagcaccag gtgcgaggta tatataggtg tagcttttgc ggagaagctc
    15901 aaatttttca gtatcaattc ttgaattgta atgaatatta actcccagat ctgcgacgcg
    15961 tttaaaatcg ctgcgaatag cctcatcgct caagcggaac ccgggaacag caaattgaac
    16021 catgcccccg gcctgtgatt tttcttcgaa aacatcaacc tgaaaaccgg caagtgcaag
    16081 ataataagca catgataaac cgctgggccc tgctccgaca acagcaactt ttacgtcgtt
    16141 ttttgctccg tacacgtcgc gctgaacttc gttctgttcc gaaataaacc gctttacctc
    16201 cctgatatgc agtgctgaat cgtagttgat tcgtgtgcat ttattctgac acaaatggtc
    16261 acagatcata cctgtaattg cagggaatgg attggtttgg agaattaccc tgtatgcgct
    16321 tttgaaatcg gattgcgaag tgtggtacat gtaattggga atatcctggt ttgttgcgca
    16381 gctgtcttta catggtgccg atatgcaatc gaacttaccg agttgccgct gggttttaat
    16441 tgatggagtt gtaatatatt ccctgcgata ccgactcgac tggatggttt cgttaaagta
    16501 tgttgacagg ttaatcgact ttgcctgagt tatatcatcg acacctgcag tagcaaggat
    16561 gaaatctttg atatttgaaa cacccttatc agagaagttc ttggaaagtt cttcgaaata
    16621 ctgattcagc cgcatatagc cgcccggttt aagcagatcg gagcaaacgg taatggtttg
    16681 gaacccgcag gaaattaggt ctgaaatatt aaatgcatcc actccggcag aaaacgaaaa
    16741 gggaatttcg ccctgcaaat catcgtttag tttctttgcc agtgcaatcg acactgggtg
    16801 aagtgcccgt ccgctcatgt acatcatttc tacgttgtcg ctgaaaatat ttttgttatt
    16861 taccgattca agcgtattcg taagcttcac accgaaacaa agccggtgat cagttgcagt
    16921 tttctgcaat gatctgatga tttttaaagc atccgggtat tttaaatcgt gctcaaaggc
    16981 aatatcaggg acttgagttt taaatttaga cgtatggttg agaatttgtc gcaattgatc
    17041 tggacccaac aaggtggggt tgagcttaat gtatgtatgc agcttttttt cggtaagcag
    17101 atagaaagcg atactttcaa tttcgttggc agggcatccg tgcatcgtcg acagagtaat
    17161 gttatccgaa attttcgagg ggatattcaa tgcagtaatc tcaggataat aatccttaat
    17221 actttcaagt ttttccgtaa gctctaccgt gcaatcgttc attttttcga agaaccattg
    17281 cacatttgcc tgcataatac cttcgaggtt atagccaacg ctcatgttga aaacggtatt
    17341 gacgttgttg gaaaaaccca gttcctgatg aaggatatgt attataatcc aggcattaag
    17401 gtattcattg aagctttggt ttattctgag ttcctgcgac cattcacagt tatagccttc
    17461 gtcctgcata tcgatacatg gtttcggcac ctccagttca tcaagtgtct gaatcgtttt
    17521 tagttcaatg tatcgggcac ccattagcca tgctgcaata atattttgtg ccatttgaga
    17581 atgcggacct gctgcaactc caacgggcgt gtcaatcaga gtattgaatt gtgttgtttt
    17641 atacgtgtta gtactatcgg gaataaagaa taattccgaa ggaataccga atacactccc
    17701 cgaagtgtgc aattcattaa gaatgatctg cagtagtttt tttaacggaa ttggattgaa
    17761 attgccggac atagattttt gtttttacaa actttgtgta gcaaatttaa tgaaaactac
    17821 cgacattagt gttgtgatgt taaaaggtta agcacaatta cgacaagaac tggaagcaat
    17881 aaaaccacat tatattgatg aaaatatctg ataatgctgt aaactggcgg ttttataaga
    17941 atacagtaaa tgtagcatgc aatttgcata cgatggcaat aaactttttt ttaagcgatt
    18001 tgttaagatt ataaattata aacgccggaa ttgtagtagt ttcgtttttt taatacattg
    18061 atatttgtga atataaagct ggctctttta gtaaatttaa taatgacact ctccggctct
    18121 gtatttggcc aggctgttaa aatcagtgtt accgacacgc accgtgaccc tcttgtgggg
    18181 gctactattc agatgataaa cgtagttgat tcaactcagt tttttgctac cacaaatcag
    18241 tatggttccg gtattattaa cgatgttgaa caaggtcttt atactatcaa aataagctat
    18301 atcggttata aacccattga aaaaaacatt agcattaaac ctgacaagcg tgaattctca
    18361 tttcagttgt cggaagatgc cattgcgttg aacgaggtta cggtagaagc ccgaaaacca
    18421 ctgatcaggc acgaagacga taaaatgatc atagatccgg agcctctggc attggttact
    18481 accaataccc tggaagtgct tgaaagtact cccggaattt atgtcgatca ggatggaggg
    18541 atttatctga acggagcttc tcccgctgca atttatatca atggacggga acagaaaatg
    18601 agtagtcagg atattgcaat gatattgcgc agcttaccgc ccggaagtat tcagagtatc
    18661 gaagtgctca gaacaccttc tgcaaaatat gatgcaagca gctccggtgg cattgttaat
    18721 gtgatactga aaaaaggagt gaaaatcggt cggtttatat caatccgtgc gggtatgaac
    18781 cagggtaagt atggtaaccg ttttgcaggt tttaactata acgatggcag cgacctcacg
    18841 tcgtactatg tgaacggcaa ctacaccttt caggatatga ttgaggattt gaattccgaa
    18901 aggatgctga atgccgatac tttattgaac cagaatgcgc aaacgcggag aaacagccat
    18961 caggcatacc ttgggtatgg aatcaattat tatccgaacg ataaaattac ttacaattac
    19021 gatggccgta ttaatgcaag ctttccgtat tcggcgtcag ataattacaa tatagtggtt
    19081 aatggttcgg aagaacagtt atcggaaagc gaaaccatga ctaattcgtg gaatgatgct
    19141 tttaatgtgc agcaggagtt cggaatggta tataaaatag atacaatttc atcgaatatc
    19201 gacaccaaac tgagttataa tttcaaccat aacaacgctt ttcaaaacta cagttcaaat
    19261 tattacttcc cgtttgaagc aagcgttttg ggcgaaggca atcacttgca aaacaggcat
    19321 tttgtacagt ttcagtcaga ccttacttac cagtttccgc ataaaattaa gctggaaagc
    19381 ggtgttaaga cctcctttca gcattatgcg agcgataatg agtatttttt taatgtaaac
    19441 cagagcatga ttgcagaccc cattagaacc aatgcttttt tttataacga aaacataaat
    19501 gctgcttatc ttcagttatc gaagactttt tggaaacacc tgcagctgaa aaccggagta
    19561 cgcatagagc acacatttat gaacggagta cagactgtac cgaccgatac cagtttttta
    19621 ctgaataggg cagattggtt cccctacgta tatttgagtc ggcgcgtgtt ggaagttaaa
    19681 ggcttcgaag ccagggtgtt tctaatctac cgtaaaacaa taaaccggcc gggctaccaa
    19741 aacttaagcc ccaatataaa atatgtcgat gactttttat atgaaaccgg taatcctgaa
    19801 ttaaaaccgg aatttaccga taattttgag ttgaacataa gtgtcgaaga catgcccttg
    19861 tttgctgtcg gaagaagtta tacacgcgat attatttcga atgtattata taccgatgat
    19921 aactacccca atattgttgt gaacacctac gataacttaa gtaacagcac cgaaacctat
    19981 ttaaggggta tggccggaat tcccccgggt ggccggtatt attttgctgt gggagcacaa
    20041 tataacctga atgagtatga aggcttttac gataataccc cattttcata caagcggggc
    20101 agctggcgtt tatttacgtt tcattcttta aagctattca aaaatacgcg aattacatta
    20161 agcgggttta tgatgattaa tgggatgcaa aatttttatg agctgaaaaa tttcgggcag
    20221 gtgaattttg gtatcacaca acgctttttc gacgatagac ttactattag cattaatggc
    20281 agggatatac tcagaacaat ggtaacacag tttgagatgc atcagggaag tatttcggtt
    20341 gcaggctcac gctattccga caatcagcgc tttggtattc agattcggta taatttcggt
    20401 cttaaaaaac cggagaagaa accaaacgaa ataaaatacg atatcgacga ataaacgatt
    20461 caatattttt agatgtttct gtgagcgttt ctttacgcca tgtagaattt gataaaccaa
    20521 gattgcattt atgttatact ggcgaataaa tgcatactcc aatagtgtaa tcaagttata
    20581 gtactgcgaa ttctttttcg ttgtagcgtt ttgcaacttc aatagaaatc aaatagtcgc
    20641 ttgtttaaaa aatatactaa aatataaatt caaagaaaaa tttattaata tgggtattgt
    20701 aattggttaa ataattatat aaattagcaa agttgtgttg tttctgcacc tgatgggtaa
    20761 tttagtaatg taccatagtt gcggattgtt tttcggtgaa aattaaaaat gtgatattat
    20821 tggatttgtt aattaaattt tatgcttatg aatacaatta gactatttca ggattttaca
    20881 caaaaaaagt cgataatcag tcggttcttt tttgttttta tgctaacact tttgttacaa
    20941 cttccagcga atttatttgc tgataataca gtaactattg gttcggggac taataccggt
    21001 tatctagttc cagcaaacac ttactacaat tattcatata cccagcaaat tgtattgcaa
    21061 agcgaaattg ctacagccgg gcaaataaca aagatcaggt tttatatggt tgggggtatt
    21121 tcgctcagta attcaaacaa ctggactatt tatcttggtc ataccacaaa aacatctttt
    21181 tcatccacaa ctgattgggt cccgctggca agtatgactc aggttttttc cgggaccatt
    21241 gctgcgacgc ctgttgccgg gtggtatgaa atagtgctta cgggtacttt tatatataac
    21301 aacaccgata acctagtggt tgctgtggat gaaaatgcaa ctggtttaaa tggcagtacc
    21361 aattatgtaa ggatatggac aacaccggtt gccaacagag cgatgcatta tcggtcagat
    21421 ggtacaaacc ctgatccggc aagtccacca actgcaacag gcaggttatc atacatgaat
    21481 cagatgcaac ttgatattgg agccccaccc acttgtttgc aaccaagcgg tctctcaacc
    21541 accaacataa catcaagttc agccacggtt aactggactg cctccacatc gaatccttcg
    21601 ggcggatatg atatttatta caacacctca agcacagccc ctactgccgg aaccacccct
    21661 ttggaaagcg ttgccgcagg cgttacaacc tataatatga gcagccttag tgcagcaaca
    21721 acctattatg tgtgggtacg tgcaaactgt ggcggtggtg atttaagttc ctgggcagga
    21781 tatacgagtt ttactacggc atgtaccaat gccgcactaa cagtaaacga gggttttaac
    21841 acatcaggaa catctgtatt tccaacttgc tggtctcaac aatatgtaac aggaacatct
    21901 gccctgagtt tcctaacatc gggtagctat ccaactgtat cagcacccta cgaaggaacc
    21961 cgaatggtat attggaatag ttttaattat tcaaatggtc agcaaacaag attggtttca
    22021 acaccaataa cgacaaccgg aactgcgagt gttgatgttg aattcagatg gtatcacgga
    22081 accgatggcg gatcatcttc atttcaaacc gaaggtgttc aggtacaata ttccactgat
    22141 ggcagcacat gggttaatgc tggtagtttc ataaaaaggt acgatgcaac agcagggtgg
    22201 agtttaaaga caataacttt gccagttgga gcgggaaatc aaccgactat ttatgttggc
    22261 tttttgttta catcaaatta tggatataat tgctttcttg acgctgcaag cataaaaccg
    22321 tctccaagtt gcctaactcc aacagcgttg atgtcatcac caacttcgac aacaagtgga
    22381 accgtttcgt ggactgcttc cacctcgaac ccttc
//
LOCUS       DAOOQP010000038        22325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2828_length_22325_cov_5.325595, whole genome shotgun sequence.
ACCESSION   DAOOQP010000038 DAOOQP010000000
VERSION     DAOOQP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_22325_cov_5.325595"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLK75_05750"
     CDS             complement(<1..918)
                     /locus_tag="PLK75_05750"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOY38787.1"
                     /translation="MISGNVKNKTAVIIGAGPAGLTAAYELLKHTDIHPIVLEKSHSI
                     GGISKTVDYKGNKIDLGGHRFFSKSEEIMNWWQQVFPLCNSPENIAKNDTWDTGSDNV
                     FLQRDRLSRIYYLRSFFDYPVELNKNTVRKLGLIRILKIGFSYLKSRLFPIKHEKSLE
                     HFFINRFGKALYETFFKDYTEKVWGIPCSKLSAEWGAQRIKGLSASKVLTHYLKSFFS
                     KKKNDVSQKNTETSLIELFLYPKYGPGQLWEAIAVDIAQKGGDVQLLCEFSSVRIGNQ
                     GKFHVEYLNKNTGETTAIQDVDYLFSSAAV"
     gene            1032..1598
                     /locus_tag="PLK75_05755"
     CDS             1032..1598
                     /locus_tag="PLK75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY38788.1"
                     /translation="MKKLSVLFAFLLAGIICFAQENPKKNQAADKKDKTNKQENPTKP
                     TDIAFEKTTYDFGDIPFKSEAKAVFVFKNISAKPIALTNVKASCGCTVPEWSKEPVKK
                     NKKGQIIVTYDTQRIGNFNKTISVFTDRQDNPIQLQIKGNVLAPEAGTPEHEQYQEKQ
                     QIKKGEPKQMNGKKGEPQGKKIDEQPKN"
     gene            1606..2805
                     /locus_tag="PLK75_05760"
     CDS             1606..2805
                     /locus_tag="PLK75_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY38789.1"
                     /translation="MPLISEKGLAMPASPIRKLVPYADAAKARGVKVYHLNIGQPDIK
                     TPDTALQAIRNFSDKVIEYSHSAGNASYRKKLAESYKKIGLDVDAADILVTTGGSEAI
                     TMSLMTTMNPGDELIVPEPFYANYNGFAITAGVKIVPVTASVENDFALPPMADFEKLI
                     TPRTKGILICNPNNPTGYLYSKAELLVLAELIKKYDLYLFSDEVYREFCYDGNEHFSA
                     MLLKGIENNVIMLDSVSKRYSMCGVRVGCMISKNKEVMCTALKFAQARLCPPSLGQVA
                     AEAAIETGSEYFKEVYDEYIKRRDFMVARLNEMDGVYCPKPKGAFYTVVKLPIDDSDK
                     FAQWLLDSFEYKKQTVMVAPATGFYNTHGLGKNEVRIAYVLKTEDLADAMETLSEALK
                     VYPGKTN"
     gene            complement(2903..3877)
                     /locus_tag="PLK75_05765"
     CDS             complement(2903..3877)
                     /locus_tag="PLK75_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOY38790.1"
                     /translation="MNKCFFVFLRHICLGICIIAFIVACQSTEKQRKLRIIHAGSVSV
                     PLAEIVDSFISLHSDVVIETEAWGSKAGARRIADLQQPCDVFVSADYKVIDRILIPDH
                     ASWNIPFASNEMALVYTNKSGYSAEINTENWMDILMKPDIIFGRSDPNSDPCGSRAVL
                     CIKLAEKYYNRPGLSEQMLSLHLNQIRPKETDLLALLEKDAIDYIFLYKSVAIQHQLK
                     FLELPDEINLSNSELDSLYNTVTVEVAGNSPEKKITETGEAMVYGITIPLKTTQKELA
                     EEFVDFFLNYNQGLKIFEKHGQLTIVPSKTQSYANIPERLKKYALPPR"
     gene            complement(3951..4232)
                     /locus_tag="PLK75_05770"
     CDS             complement(3951..4232)
                     /locus_tag="PLK75_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOY38791.1"
                     /translation="MFKTNEYFDGKVKSISFNTKKYPATIGVMEKGEYVFGTSSIEHM
                     TVTSGKMDVLLPGETEWQTYRKFETFIVPKDVKFKVRVEGDTSYRCLYK"
     gene            complement(4255..6411)
                     /locus_tag="PLK75_05775"
     CDS             complement(4255..6411)
                     /locus_tag="PLK75_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOY38792.1"
                     /translation="MNIDDLKRNHKKISEYKYQWAPIEKGYNNRTLYVNVGTGEINEK
                     PVTKLMKEKFIGGKGFGLKLLWEATKPSTKWNDPENEVVISGGPCCGITQYSGAGKSI
                     VVSISPQTDSIMDSNVGGYFGPFLKFCGFDAIELHGKADKEVIVYIDEETETVQIFEA
                     PLEAIDSHILGEQLTEMFANDEKDKINVAVVSAGTAADHSLIGMLNFTFWDPRRKVAR
                     LKQAGRGGIGTVFRDKRIKALVAKVKGIKGNLNNVVDLPAIAERGKRFNKEMKDFDDT
                     QCQMRKIGTAHLTNIMNDYDLFPTHNFKFGSHPDAGNVHSEIYKSLFTQNMPDGCWIG
                     CNMSCAKSVDNYELQTGPYKSQRVTVDGPEYETAASLGSCAGIFSPDFTIEANFYCDT
                     YGICTITWGTILGFVMECYENGIINKERTGGLELNFGNADAAMELLHQLARGEGFGVV
                     AGQGVRKMKQLFADNGWGDPQFLQDIGMENKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFENKAEALHYFPMFRTWFGLAGFCKLPWNDVEPESNKTAD
                     EPAKVPEHVDNYVTIFNSVTGLSIDKHELIRQSERVYNFQRIFNLRRGYGTRKHDAQP
                     YRAAGPVTVEEYESRAERYDKQMKELIGIDPVGKSTAEKVAITRKYREDRYEKLLDAV
                     YHRRGWTKNGVPKIEHLKNLGMDLPELIEVIKDHQE"
     gene            7006..7563
                     /locus_tag="PLK75_05780"
     CDS             7006..7563
                     /locus_tag="PLK75_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY38793.1"
                     /translation="MSNSQLLLKAASFAAARHQNQKRKGASAKPYINHPIKVAEILAS
                     NGEADNTNLLIAALLHDVLEDTVKTPDEMIEVKSVIHDYFGANVLSIVEEVTDDKSLP
                     KQMRKQMQIEHAPHLSVNAKKLKLADKINNLTDILHDPPTDWPMERKYEYFVWAEKVL
                     NGLSGINPTMEGMAKELVIKGKHCY"
     gene            7673..8266
                     /gene="thiF"
                     /locus_tag="PLK75_05785"
     CDS             7673..8266
                     /gene="thiF"
                     /locus_tag="PLK75_05785"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOY38794.1"
                     /translation="MNYLSFDEIKKRLSQFKVGIAGCGGLGSNCAVALARTGIGSLVL
                     VDFDKVSEGNLNRQYFFTDQLGQKKTEALRYNLNKINASCHIETHNLKIQANNVLSVF
                     SGCDVIVEAFDAADQKKMFIETCMSQLASTPLVCGIGLAGYGSNNMLSTVQYDKNLYI
                     CGDQTYEVFANLPPLAPRVGIVANMQANQVLEILLKQ"
     gene            8271..8474
                     /gene="thiS"
                     /locus_tag="PLK75_05790"
     CDS             8271..8474
                     /gene="thiS"
                     /locus_tag="PLK75_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOY38795.1"
                     /translation="MNILLNNNPVNFTKSEMSIAEIIEEKNFTFKMLVVKLNGILIEK
                     ENYRSALVKDGDTLVILHLISGG"
     gene            complement(8575..9555)
                     /locus_tag="PLK75_05795"
     CDS             complement(8575..9555)
                     /locus_tag="PLK75_05795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY38796.1"
                     /translation="MFKTKVKFDPKSLRFEKVPFSLKRLIMLLIPHSFVSLISAVAML
                     TVYMVFFETPEEQLLRAENRYLRKNFKEMSSKFTEMNVLLEDMAGRDNELYRSIYQLD
                     SIPQAVRNSGFGGSQAHEDLEGYTSSWLVIDVANKIDVLSKKLNIQANSYKEITEMAM
                     LRTKRLQSIPVLQPINNSQLNHIGSFYGYRKHPVFNTIQMHHGIDYVAETGTPVFASG
                     DGVVTRIEKNRSRSGYGNLVIIDHGVNGLSSYYAHLNSIDVNKNDKIKRGQQIGTVGN
                     TGLSTAPHLHFEVRINGESVDPLKYIISVTPKQYDDLLKMSKMNGGVSFD"
     gene            9670..12291
                     /gene="alaS"
                     /locus_tag="PLK75_05800"
     CDS             9670..12291
                     /gene="alaS"
                     /locus_tag="PLK75_05800"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOY38797.1"
                     /translation="MESKQLRSAFLEFFSSKQHQIVQSAPMVIKNDPTLMFTNAGMNQ
                     FKDYFLGNRVPQHRRVANTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     NEAIEFAWEFLTEVLKIDKSRLYVTVFAGDTKEYLDEDIESRECWKQLIEHDRILDGT
                     RKDNFWEMGETGPCGPCSEVHIDLRFDDDRAKTDGKELVNKSHPLVIEIWNLVFIQFN
                     RKSDGSLESLPEKHVDTGMGFERLCMVMQNKQSNYDTDVFTPIINRISEIVGFKYKAA
                     DKTDIAMRVVADHLRAVSFAIADGQMPSNVKAGYVIRRILRRAVRYYYSFLSYQQPLM
                     HKLVPVLSDTMGEAYPELKAQQSFIEKVIYEEENAFLITLEKGIRMLDAIISDVKKND
                     YRIISGTDAFTLYDTYGFPFDLTDLIARENGLIVNRKEFETAMEKQKTMARNASAVDT
                     GDWNILIEDEIEEFVGYEMLETPVQITRYRQMNQKGKNYFQLVFNITPFYAESGGQVG
                     DTGKIENENETVEIVDTRKENDTIIHISEKLPHDVSAIFTAVVDKERRNDIRRNHSAT
                     HLMHYALRNVLGTHVEQKGSLVHPDYLRFDFAHFQKMSENEIRDVEIMVNTLIRDNII
                     LDEKRNLPLIKAKNMGAMALFGEKYGDIVRVVQFGESIELCGGTHVDSTGSIGFFRIV
                     SESAIAAGIRRIEAVTGRVAEHLIFENIDMLRQIGALVKVQKNYPEAIQSMIDENKKM
                     KSVIDEFNKKEIILVKNRLKTQVKEQNGIKYITDVLDNMDAAALKDMSYQLKSEIRGL
                     VCILAGISANKVYLSVMIDEEIMQAHNLNAGLVVREAAKAIQGGGGGQPFYATAGGSK
                     TEGAKDAVNIALQMCIS"
     gene            12414..13106
                     /locus_tag="PLK75_05805"
     CDS             12414..13106
                     /locus_tag="PLK75_05805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY38798.1"
                     /translation="MKTMKFVLLAAAGLFAMSSCQSQKKSTDEKISLSNSKDSASYAI
                     GMQIANNFKSNGIDTDINMEAVIAGLRDNGTGKTQFTIETADQVVQRYFASISESKNA
                     GKVEEGQKFLAAKEKEPGVKKTASGLLYKEITPGTGATPKATDKVKTHYRGTLIDGTE
                     FDSSYKRGEPAVFPVNQVIKGWTEALQLMKVGAKWELYIPYNLAYGERGAGQLIGPYE
                     TLIFEIELISIE"
     gene            13181..14089
                     /gene="pyrB"
                     /locus_tag="PLK75_05810"
     CDS             13181..14089
                     /gene="pyrB"
                     /locus_tag="PLK75_05810"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOY38799.1"
                     /translation="MKNKSLISINDYTKEDYMRILNLAAEFEKNPRQNILQGYVIASL
                     FFEPSTRTRLSFESAVQRLGGRIIGFSEAGNSSVSKGESLKDTILTVCNYSDLIIMRH
                     PVEGSARFASEVASVPIINAGDGANQHPTQCLLDLYSINKTQGKLDNLSIAVVGDLKY
                     GRTVHSLIHAMINFNPVFHFVSPPTLRLPEEYKQLLRDNKIPFYEHDEITDCIRESDI
                     MYVTRVQKERFSDPMEYEKVKNTYILHNSMLEGTRDNLRILHPLPRVTEININVDPSP
                     KAYYFTQALNGVYTRMAIISIILGLK"
     gene            14097..14561
                     /gene="pyrI"
                     /locus_tag="PLK75_05815"
     CDS             14097..14561
                     /gene="pyrI"
                     /locus_tag="PLK75_05815"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOY38800.1"
                     /translation="MKHNNSEMTVRAIENGTVIDHIPSKNLFKVLSVIGIERMDTTIF
                     IGNNLDSKKLGKKGIIKITDKFPSLDDINKIALLAPNAVFNIIRNYEVVEKKPVSLPE
                     KVQGFVKCMNPKCITNFEQVPTHFHVSVSDGEIQLKCKYCEKITGERNIEVI"
     gene            14568..15749
                     /locus_tag="PLK75_05820"
     CDS             14568..15749
                     /locus_tag="PLK75_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOY38801.1"
                     /translation="MKKHIILFSSCVLLAVGSLAQSTDNAANRNNTFAFTMLNKLYSE
                     TKNTLVSPLSISVAMAITYEGAKGKTSKEIATVMGFDKNKKQHHVQFVDLVQQYRDTS
                     LHALKMNNILMVQKDYAFNKDFIAASLDYSAMLYFANFRDDTQREAARRGLNDWVESQ
                     TNNTIKDLLQPGILDNLTRLVIVNSLYFNANWEIAFDAKKTRQMIFYSIDKQVIVPFM
                     HGREDFNYYADSLIQVLELPYSGKRFSMVVFLPKNEISLKNFIGSFTVETYNFYISQM
                     AKHRVDVLLPSFTISDESNLRQFFESLGMVTAFTGYANFKGMNGKKDLMIKDVVHKTY
                     IKVDETGTEAAGASAVVIREKSAPAEKPVFFNANRTFFYVIRDNASNSILFAGSINKP
                     F"
     gene            15852..16376
                     /locus_tag="PLK75_05825"
     CDS             15852..16376
                     /locus_tag="PLK75_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38802.1"
                     /translation="MKQILTLLMVALLFVACGNCKKCKDDDKCCDSTKNECCKHPENL
                     VMVDSLLTNQEAFLDKNIAVCGVAIHVCEHSGKNLFIATDTAAEDYLVAKATADLGVF
                     DKALLNEHVCAFGTFKKAEVEAVETHHEKEVYYYLQCDSVKQCKCKGDNKKCGDAAKS
                     GCKGHSEKHNCEGQ"
     gene            complement(16740..18863)
                     /locus_tag="PLK75_05830"
     CDS             complement(16740..18863)
                     /locus_tag="PLK75_05830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOY38803.1"
                     /translation="MKRLILICLPLILLAGACKNKKATDMQPENPFFTAFDTPYGVPA
                     FDKIKNEHYLPAFKEGIKRHEQEIKAITDNKEAPDFENTILALDQSGEMLTTVSSVFF
                     NLLSAVSSDSLQAIAKEVTPLVTDHQNNIMLNEDLFKRVKAVYDSRLEAGLSPDRIRV
                     TEKYYNDFIRSGAALSAADKETLRTLNNELSMLDLQYGDNLLAETNVNFKLVVDKEAD
                     LAGLPADVIAGAAATAKDQGLEGKWVFQLSRASMTPFLQYAQNRDLREKIYRGYFMRG
                     NNGNDNDNKKLITQMVEKRAKKAQLLGYKNYAEYVIAENMAETPENVDQFLMKLWKSA
                     LPVASQELKEMQAIANKEGANFKIASWDWFYYAEKLRKQKYNIDENELKPYFKLENVR
                     DGVFWVATQLYGIKFNKVTNIPVYHPDVEVFEVKEADGTLIGILYMDYYVRDSKQGGA
                     WCTGFREAGYDKDGKREIPLVSIVCNFPKPTADNPTLLSWDETTTLFHEFGHGLHGLF
                     TDGYYKRTAGVTPRDYVELPSQIMENFASEPEVLRHYALHNKTNEPMPEELIQKLVNS
                     GLFNQGFATVEYLAASLLDMKWHLIESGASVDDVLKFEEEAMNNIGLIPEILPRYRST
                     YFSHIFSGGYSAGYYVYIWAAVLDADAFAAFAESGDLFNKELAAKFRKYCLSEIGEAP
                     AMEQYKKFRGQEPNEKYLLERRGLK"
     gene            complement(19268..19693)
                     /locus_tag="PLK75_05835"
     CDS             complement(19268..19693)
                     /locus_tag="PLK75_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOY38804.1"
                     /translation="MKYSYQYPRPMVTVDMVVIATSVSHSVLLIRRKNEPFAGRWALP
                     GGFVDMNESLEIAAARELLEETGLSLANLEQFKAYGNVDRDPRGRNIAVVFYSIIPEA
                     VTVTGNDDAIEAGWFDVNDLPELAFDHKRIITDLLLKIQ"
     gene            complement(19802..20440)
                     /locus_tag="PLK75_05840"
     CDS             complement(19802..20440)
                     /locus_tag="PLK75_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY38805.1"
                     /translation="MDIRRLIEQGEGQQLDFKYCVNDAHKIARTLTAFANTNGGTLLL
                     GVKDNGKISGVASDEEYYMIEKAAQTFCKPEVPFRCKTHRCNGKNVFEVIIPSTGFVP
                     HYAPDENNKWLVYIRRNDQNLPANRILLEYKKRLNSGNITAIALDRNVEMLLNYLNRN
                     GAITFDVCRQLTNMNKFSTENFLVNLLLMNLIEMDINEKETVFCLKNIPDNR"
     gene            complement(20496..>22325)
                     /locus_tag="PLK75_05845"
     CDS             complement(20496..>22325)
                     /locus_tag="PLK75_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY38806.1"
                     /translation="VYSSCVNLKKQLIAFSDEIGKVIYIYDFNGFKIIKKLSGHADKI
                     NDICFSNDGSMIASCANDGLIKIWDISKGTAISFKSNTKHVSRVQFFDNDRKVISASE
                     DGVIIYWDIATQKQLASIYPVGDSLTVVVTPEGYFDGTPEALRYLYYVQGTDIIPLEA
                     YYEQFYRPNLLQRILRGESIEQSKVDFNKRKPNSGVKITEPAQGDYRSINMPVPTNAN
                     LLKLKASFTDNGGGVEEVRVYRNNKLVKSEIIGLNKPGESISRSFDVPLMPGINTITV
                     SVFNSDRTEICDTIRMNFSGTITETADLYLVSIGINEYENPQYHLNFAVSDALSFSQA
                     IETGAKTLFGNIYTYNITDRDATKANITKILNEVQSKAKPWDVFVFYYAGHGAAIAEE
                     GRTNFYFINADVTNIYSKEQLTGKAISNSELLFFSRDIQAEKQFLVIDACNSGAAGDL
                     LAYRSGPEEERAIAVLARSTGTHFLFASTANQLAKEIPEIGHGVFTYSLLKAMTGGDG
                     LYPEGKSLTVKDISNYTEKQVPELSVTYTPTKTPQHPTAYSYGQDFPLVITGNLLDFK
                     TLKGKYDDYSIEQLQEMKAKAVQEEDYMKANELKQEIEKRSNK"
BASE COUNT         6583 a         4643 c         4630 g         6469 t
ORIGIN      
        1 tacagcagct gaagaaaaca aataatcaac atcttgaata gctgtagtct cgccggtgtt
       61 tttattgagg tattcaacat gaaacttgcc ttgatttccg atgcgaaccg aggaaaattc
      121 gcaaagcaat tgtacatcac ctcctttttg agcaatatca acggcaatag cttcccacaa
      181 ttgtcccggg ccatatttcg gatagagaaa cagttctatc aaactcgtct ctgtattttt
      241 ttgggacaca tcgtttttct tcttcgaaaa aaacgatttc aggtaatgtg tcagtacttt
      301 cgacgccgac aaacctttga ttcgctgtgc cccccattcg gctgacagtt tgctgcacgg
      361 aataccccac accttttcgg tgtaatcttt aaaaaatgtt tcgtacaacg ctttgccaaa
      421 acgattaata aaaaagtgtt ctagactttt ttcgtgctta atcggaaaca ggcgactctt
      481 cagatagctg aagccaattt ttagaatacg tattaaacca agcttacgaa ccgtgttttt
      541 attcagctca accggataat cgaaaaaact tcgcaagtaa tatattcgtg acagtctgtc
      601 gcgttgaaga aaaacattgt cgctaccggt atcccatgtg tcgttttttg caatattctc
      661 gggactgtta caaaggggga acacctgttg ccaccaattc ataatttcct ccgatttcga
      721 aaaaaaacga tgccctccaa ggtcaatctt attgccttta tagtcaacgg ttttcgaaat
      781 acctccaata ctatggctct tctcaagaac aattggatga atatcggtgt gtttcagcag
      841 ctcataggct gcagtaagtc ctgccggtcc agctccgatt attacggccg ttttattttt
      901 cacattaccc gaaatcatag ctgccaaaat tagtaaaatt attcgagctt gctttatggc
      961 atataatcag taaaatttgc ataatgtttg taaatatttc ataattttga aaaaattaat
     1021 ttatactaat tatgaaaaaa cttagcgtat tattcgcatt tctgctggca ggcatcatct
     1081 gctttgcaca ggagaacccc aaaaagaatc aggctgccga taaaaaagac aagaccaaca
     1141 agcaggaaaa cccaaccaaa cccaccgata tcgcgttcga aaaaaccacg tatgattttg
     1201 gtgatattcc ttttaaaagt gaagcaaaag ctgtgtttgt ttttaaaaac atttcggcaa
     1261 agcccattgc actcaccaat gtaaaagctt catgcggttg tacagttccg gagtggagta
     1321 aagagcctgt taagaaaaac aaaaagggcc agataattgt aacatacgac acccagcgta
     1381 ttggaaattt caataaaaca atttcagtat ttaccgaccg ccaggataac cccattcagt
     1441 tgcagataaa gggtaacgtg cttgctcccg aagcaggaac tcctgagcat gaacagtatc
     1501 aggaaaaaca acagataaaa aaaggtgagc ccaaacaaat gaatggaaaa aagggggaac
     1561 cacaagggaa aaagattgac gaacaaccta aaaactaaag taaacatgcc attaatttca
     1621 gaaaaaggat tggcgatgcc cgcatcacca attcgtaaac tcgttcctta tgccgatgca
     1681 gcaaaagcaa gaggcgtgaa agtgtaccat ctcaacattg gtcaacccga tattaaaact
     1741 cccgataccg cattgcaggc catccggaat tttagcgata aagtgattga atattctcat
     1801 tctgccggta acgcaagcta ccgaaagaag cttgcagaat cctacaaaaa aattggactt
     1861 gatgtagatg ctgccgatat tctggttact accggaggct ctgaagccat taccatgtcg
     1921 ctcatgacta ccatgaatcc aggcgatgag ttaattgttc ctgagccttt ttatgcaaat
     1981 tacaacgggt ttgctattac tgcaggagtt aaaattgttc ccgtaactgc atctgtagaa
     2041 aacgattttg cattacctcc gatggcagac ttcgaaaagc tgattactcc gcgcacaaaa
     2101 ggtattttaa tctgtaatcc gaacaatcct acaggatatt tgtattcgaa agctgagctt
     2161 ttggtgttgg ctgaattaat taaaaaatac gacctttacc ttttctcaga tgaagtgtat
     2221 cgcgagtttt gctacgatgg gaatgagcat ttctcggcca tgttgctcaa gggcattgaa
     2281 aacaatgtaa ttatgctcga ctctgtttcg aaacgctaca gcatgtgcgg tgttagagtt
     2341 ggctgcatga tcagcaagaa caaagaagtg atgtgcactg cgcttaaatt tgctcaggca
     2401 cgcctctgcc ctccctcact gggacaagtg gctgcagaag ctgcaataga aacgggtagt
     2461 gaatatttta aagaagtgta tgatgaatac attaaacgcc gcgattttat ggttgcccgc
     2521 ctgaatgaaa tggatggtgt atattgcccc aaacccaagg gagccttcta tacagtggta
     2581 aaactcccta tcgatgactc cgacaaattt gcacaatggc tgctcgacag ctttgaatat
     2641 aagaaacaaa ctgtaatggt agcacccgct acagggttct acaacacaca cggattaggc
     2701 aaaaacgaag ttcgtattgc ctacgtattg aaaacagaag accttgcgga tgccatggaa
     2761 acacttagtg aagcattgaa agtgtatccc ggaaaaacaa actgatcttc aatcaaatag
     2821 atgaagtcct ttgcagttat aaacccctgc aagggatttt ttcattaaat gatatcgatt
     2881 aatatacaga ataacgtatg catcatctgg gcggaagtgc atactttttc agtcgttcgg
     2941 gtatattagc gtagctctgg gtttttgacg ggacaatagt aagctgccca tgtttttcaa
     3001 atattttcaa accttggtta taattcagaa aaaaatcaac aaattcttca gccaattcct
     3061 tttgggttgt cttcaacggg attgttatcc cgtaaaccat tgcttcacct gtctctgtta
     3121 tttttttctc agggctgttt ccggctactt caaccgtaac ggtattgtaa aggctatcaa
     3181 gctcactatt gcttaggttg atttcatcgg gcagctccag aaatttcaat tgatgctgaa
     3241 tggcaactga tttgtaaagg aagatatagt cgatagcatc cttctcgagt agtgcaagca
     3301 agtcggtttc ttttggccgt atctgattca ggtgtaacga tagcatttgt tctgataacc
     3361 ctggccggtt gtaatacttc tcagccagct ttatgcaaag cacagctctg gagccgcatg
     3421 ggtcggaatt cgggtcggat ctcccaaaaa taatatccgg tttcatgagg atatccatcc
     3481 aattttcagt gttgatttcg gcagaatacc ccgatttgtt ggtgtaaacc aaagccattt
     3541 cgttgcttgc aaatggaata ttccatgaag catgatccgg aatcagtatt ctgtcgatta
     3601 ccttatagtc ggcagaaaca aacacatcgc acggttgttg caaatcggca atacgcctcg
     3661 cccctgcctt acttccccag gcttccgttt cgatgacaac gtcagaatga agggaaatga
     3721 acgaatcaac gatttcagct aaaggaacac taacacttcc ggcatgaatt atcctgagtt
     3781 tacgttgttt ttcggtgctc tgacatgcaa caataaatgc aatgatgcaa atacccaaac
     3841 aaatatgcct taaaaagaca aaaaagcact tattcatatt tcgttatgat aaattttgca
     3901 cttcgatatg gcaatataag ccgtaatttt tacggcttat cattgtaata ctatttgtac
     3961 aaacatcggt acgatgtgtc gccttcgacc cgaactttaa atttcacgtc tttaggaaca
     4021 ataaaagtct cgaatttcct ataggtttgc cactcagttt ctccaggtag caaaacatcc
     4081 atctttcctg atgtaacagt catgtgctct atagaagaag ttccgaaaac atattcgccc
     4141 ttttccatga cgccaattgt ggccggatat ttctttgtgt tgaaagaaat cgatttcact
     4201 ttaccgtcga aatactcatt tgttttaaac ataattctat tattttgatt ttaattattc
     4261 ctgatggtct ttaattactt ctataagctc cggcaaatcc attcccaggt ttttcaggtg
     4321 ttcaattttt ggtacgccat tttttgtcca tcccctgcgg tgatatactg catcaagtaa
     4381 cttttcgtaa cgatcttcac gatattttcg ggtaatggcg actttttcgg ctgtggattt
     4441 tccgaccggg tctatgccaa tgagttcttt catttgttta tcgtaacgct cggctctcga
     4501 ttcgtactct tcaacagtaa cgggtccggc cgccctatat ggttgtgcat catgtttacg
     4561 ggttccataa ccgcgacgca gattgaaaat acgctggaag ttatataccc gttccgactg
     4621 acgtattaac tcgtgcttat cgatgctcaa tccggtaacg gagttgaaaa tggtaacata
     4681 gttatcgaca tgctctggaa ccttggctgg ttcatcagca gttttattgc tttcgggttc
     4741 tacgtcgttc catggcaatt tacaaaagcc ggccagtccg aaccaggtgc ggaacatcgg
     4801 gaaatagtga agtgcttcag ctttgttttc gaaggtcggg atttgattgt ttaccatatc
     4861 cataaagatc agccatgctt catcgtgttg cggccctttg ttggtcatag cgtaaccacc
     4921 ctgctgtgcc aatgattctt tcgatacata ctgtgagtat tcaaggcctt tattttccat
     4981 accgatatcc tgaaggaact gagggtcacc ccatccatta tcagcgaaca attgcttcat
     5041 cttccgcacc ccttgcccgg ccaccactcc aaatccctcc ccgcgtgcca attggtgtaa
     5101 caattccatt gcagcatctg cattcccaaa atttaactcg agccccccgg ttctttcttt
     5161 gttgataata ccattctcat agcactccat tacaaaaccg agaattgttc cccatgtaat
     5221 tgtgcaaata ccataggtgt cgcaataaaa attagcttct atggtgaaat cgggactaaa
     5281 aattcccgca caacttccga gcgatgcagc tgtttcatat tcgggtccat caacggtcac
     5341 tctctgactt ttgtaaggac cagtttggag ttcatagtta tctacacttt tagcacacga
     5401 catattacag ccaatccagc atccatcagg catgttttgg gtaaacaggc ttttataaat
     5461 ctcgctgtgg acatttcctg catcagggtg agatccaaat ttaaaattgt gagtcgggaa
     5521 caaatcgtaa tcattcataa tattggtaag atgagctgta ccaattttgc gcatctggca
     5581 ttgcgtatcg tcgaaatctt tcatttcctt attgaacctt tttccacgtt ctgcaatagc
     5641 cggcaaatca acaacattat tcaaattacc ttttatgcct ttaacttttg cgacgagggc
     5701 ttttattctc ttatcacgaa aaacagtgcc aatccctcct cttcctgcct gtttgagccg
     5761 ggcaacctta cggcgtggat cccagaatgt aaaattcagc atccctatca atgaatggtc
     5821 ggctgcagta ccggccgata ccacggcaac attaatttta tctttttcgt cgttagcaaa
     5881 catttcggta agctgttccc ccaaaatatg gctgtcaata gcttcaagcg gggcttcaaa
     5941 aatttgaacg gtttcggttt cttcgtcgat gtatacgatt acctctttat cggctttgcc
     6001 atgcagctcg atagcatcga agccacaaaa tttcagaaac ggaccgaaat aaccacctac
     6061 attgctgtcc ataatcgaat cggtttgagg cgaaatgctt accacaatcg attttccagc
     6121 accggagtat tgagtaatac cacagcaggg tcccccagag attacgactt cgttttcggg
     6181 gtcgttccat ttggtagatg gctttgtggc ttcccaaagc agtttcaaac caaaaccttt
     6241 gccaccgata aatttttctt tcatcaactt ggtaaccggc ttttcattta tttctccggt
     6301 gcctacattc acatataatg tgcggttgtt atagcccttt tctatcggtg cccattggta
     6361 tttgtattct gaaattttct tatggtttct ttttaaatca tcaatattca taaatttgtt
     6421 tttttaatgc atcattttac aaaattaaaa aaaatatcca attggtttga gctaaaaaca
     6481 tgatttatat caaattgcaa attcacgcaa ctcgcttact gcaagttatt cacaaacctt
     6541 aagtcacaaa aacagtatgt aaaaccgttc tattgctcca tttcattggg cttgcatcgt
     6601 actaaaagtc atccgttttg aattgttgtt cagtgttgaa tttggcagat accatacata
     6661 ttatgtcgca aaacatttgc agttggctcc agttaaatca atcaaaactt ttcaagatag
     6721 gtaattcaca ccacgataat aacttacagt agaacaaaac atcagattgt ttcaaaaaat
     6781 tagctaagaa aaaaatgctg tgcagctaag ggtcagaacc ctaggtaagt cttagaatcc
     6841 attcatcagg aattgatgtt tcaggctgtt tcattatacc ctctttgcca tctatacaat
     6901 tctagtaatt tatttctaaa tttgtgactt aataagaaag ccattcatgg aaaatcagcg
     6961 ttgttgttta gttttttaaa tatttagatt gaaaagtaaa aatttatgtc aaacagtcag
     7021 ttactattaa aagcagcttc ttttgcagct gcaaggcatc agaaccaaaa aagaaaagga
     7081 gcatcggcaa aaccatatat taatcatcca ataaaagttg cagaaattct ggcatcaaat
     7141 ggggaagcag ataacaccaa cctgctaatt gcggcactgc tgcacgatgt attggaagat
     7201 acagtaaaaa ctcctgatga aatgattgaa gttaagtccg tcattcatga ttattttgga
     7261 gccaatgttc tttcaatagt cgaagaggta accgacgata aaagtttgcc aaagcaaatg
     7321 cggaaacaga tgcaaattga gcatgcccca catttatcgg taaatgcaaa aaaactcaag
     7381 ctagccgaca aaatcaacaa cctcaccgat attcttcacg acccaccaac cgattggcct
     7441 atggaacgta aatatgaata ttttgtgtgg gctgaaaagg tgcttaatgg tttgtcggga
     7501 ataaacccaa ccatggaagg catggcaaag gaattggtta tcaagggaaa gcattgttat
     7561 taaagcgaaa agcattacga ttaaatgcga attaatttat aatataatga ggttattatt
     7621 attactggtt tcgttattat ttttgcagaa acaactcaat gctcagagat aaatgaatta
     7681 tttaagtttc gatgaaatta aaaaaagact ttcgcaattc aaggttggta ttgcaggctg
     7741 tggcggattg ggatcgaatt gtgcggtcgc tcttgctcgt acgggcattg ggtcgttggt
     7801 acttgtcgat ttcgacaaag tgagcgaggg caatttgaac agacagtatt tttttaccga
     7861 tcaactgggc caaaaaaaaa cagaagcact gcggtacaac ctaaataaaa ttaatgcctc
     7921 ttgccacatt gaaactcaca atttaaaaat acaagccaat aatgtgctgt cggttttttc
     7981 gggatgcgat gtcattgtag aggcttttga tgcagccgac cagaagaaaa tgttcattga
     8041 aacctgcatg agtcaactgg catccacgcc attggtgtgt ggtattggcc ttgcgggtta
     8101 tggttcaaac aacatgcttt caaccgtgca gtacgataaa aatttatata tatgtggcga
     8161 ccagacctac gaggtattcg ccaaccttcc tcccctcgcc ccacgggtag ggattgttgc
     8221 aaatatgcaa gccaatcagg tactcgaaat acttttaaaa caataacaca atgaatattt
     8281 tactgaacaa taatccggtt aactttacca aatcggaaat gagcattgct gagattattg
     8341 aagaaaagaa ttttacgttt aaaatgcttg ttgttaaatt gaacggtatt ctaattgaga
     8401 aagagaacta tcgaagcgct ttggttaaag acggtgatac tctggtaatc ctccatttga
     8461 tttcgggagg gtaatcattc ttacacaata ctgtaaaatg atattgaatt aataatccgt
     8521 taattcatta tgaataagta atcccacaag tgagccaaat aattttaaag ttttttaatc
     8581 aaacgacacc ccgccattca ttttcgacat tttaagtaag tcgtcgtatt gctttggtgt
     8641 aacacttatg atatacttaa gcgggtcgac cgattcgcca ttaatcctaa cctcaaaatg
     8701 caggtgaggc gcagtagata agccggtgtt tccgacagta ccaatctgtt gtccgcgttt
     8761 aattttatcg tttttgttca cgtcaatcga attgaggtgt gcataatagc tgctcagtcc
     8821 atttacgcca tggtctatta tcaccagatt gccataccca gagcgggatc tgtttttttc
     8881 aatcctggta accacaccat ctcctgaagc aaatacgggt gtaccggttt ctgcgacata
     8941 atctattccg tgatgcatct gtattgtatt aaatacaggg tgcttcctgt acccgtaaaa
     9001 cgagccgata tgattcagct ggctgttgtt gatgggttgc agaaccggta ttgattgtaa
     9061 cctttttgtt ctgagcattg ccatttcggt aatttcctta taagaattcg cctgaatgtt
     9121 caattttttc gacaaaacat caattttatt ggcaacatct attacaagcc aggaacttgt
     9181 ataaccttcc agatcttcat gagcctgact gccaccaaag cctgaattac gaacggcctg
     9241 aggaattgaa tcgagctggt atatcgatct gtataactca ttgtcgcgcc ctgccatatc
     9301 ttcgagaaga acattcattt cggtgaattt cgagctcatt tccttgaaat tcttacgcaa
     9361 atagcggttt tcagcacgca acagctgctc ttcgggagtc tcgaaaaaca ccatatacac
     9421 tgtaagcata gctacggcag aaatcaatga aacaaaggaa tgaggtataa gtagcataat
     9481 cagtcttttc aggctgaatg gcactttctc gaagcgtaac gacttggggt cgaacttgac
     9541 ttttgtttta aacatgtata aaaatttgct tccttttgtt atgctttgat gcaaatctaa
     9601 ttaaaacgtc taaatttgca acctgagaaa acttaaaatt atattaacga cacataagcc
     9661 aacccatcta tggaatcgaa acagttgagg tctgcatttt tggaattctt ttcaagcaag
     9721 cagcatcaga tagtccaaag tgcccctatg gtaattaaga acgacccaac ccttatgttc
     9781 acaaatgcgg ggatgaatca gttcaaagat tacttcctgg gaaatcgtgt gccgcagcac
     9841 cgcagggttg caaataccca aaaatgcctt agagtttcgg gaaaacacaa cgaccttgaa
     9901 gaagttggac atgatacata tcatcacaca atgttcgaaa tgcttggcaa ctggtcgttt
     9961 ggcgattatt tcaaaaatga agctattgaa tttgcatggg agtttttaac cgaggtttta
    10021 aaaatagata aaagcagact ttatgttacc gtctttgccg gtgatacaaa agagtatctc
    10081 gatgaagaca tagaatcgcg cgaatgctgg aagcaactta ttgagcatga cagaatttta
    10141 gatggcaccc gtaaagacaa tttctgggag atgggagaaa ccggaccctg cggaccttgc
    10201 tccgaagttc atatcgattt acgtttcgac gatgatagag caaaaaccga tgggaaagaa
    10261 cttgttaaca aatcgcatcc gctcgtgatt gaaatttgga atctggtatt cattcagttt
    10321 aacagaaaat cggatggttc acttgaaagc ctgcctgaaa aacatgttga caccggtatg
    10381 ggatttgagc gcctttgtat ggtaatgcag aataagcaat cgaactacga taccgatgtt
    10441 ttcacaccta ttattaatag gatatcagaa atcgtgggat tcaaatataa agcagcagat
    10501 aaaaccgaca ttgctatgcg ggttgttgcc gatcacttgc gtgccgttag ttttgcaata
    10561 gccgatggtc aaatgccatc aaatgtaaaa gccggttatg taatccgcag aattttgaga
    10621 agagcagtga ggtattatta ttcgttcctt tcgtaccagc aaccgcttat gcataagctt
    10681 gtacccgtgc tttcagatac catgggagaa gcctatcctg aattgaaagc acagcagagt
    10741 tttatagaaa aagtgattta tgaggaagaa aatgcgtttc tcatcaccct cgaaaagggt
    10801 atccgcatgc ttgacgcaat tatttctgat gtaaaaaaga acgactatcg tattattagc
    10861 ggcactgatg cttttactct ttacgacacc tatggttttc catttgattt aaccgatctg
    10921 attgcccgag aaaatggttt aattgtaaat cgcaaggaat tcgaaacggc aatggaaaaa
    10981 caaaaaacca tggcccgaaa cgcatcggct gtggataccg gtgactggaa tattttgatt
    11041 gaagatgaaa tcgaagaatt tgttggttac gaaatgctgg aaactcctgt acaaatcacc
    11101 cgttaccggc aaatgaacca gaaagggaaa aactattttc aactggtttt taatatcact
    11161 cccttttacg cagaaagcgg aggtcaggtg ggtgatacag gaaaaattga aaacgaaaat
    11221 gaaacagtcg aaattgttga tacccgaaaa gaaaacgata caataataca tatttcggaa
    11281 aagctccctc atgatgttag tgccatattt acggctgttg tcgacaaaga aaggcgcaac
    11341 gatatccgcc ggaaccacag cgccacccac ctgatgcatt atgctctccg gaatgtactt
    11401 ggcactcatg tagaacaaaa gggctcgctg gttcatcccg attatttacg cttcgatttt
    11461 gcacatttcc agaagatgtc agaaaacgaa atccgggatg tggaaataat ggtaaacact
    11521 cttatccgcg acaatataat cctcgatgaa aaaagaaatc tgcccctgat aaaggcaaaa
    11581 aatatgggag ccatggcatt gtttggtgaa aaatatggcg atattgtaag agtggttcaa
    11641 tttggtgaat ctatcgaact ttgcggcgga acacatgttg acagcacagg tagcatcgga
    11701 ttcttcagga ttgtttccga atcagcaatc gcagccggca tcagacgcat tgaggcagtt
    11761 acagggcgcg ttgcagagca tttgatattt gaaaacatcg atatgctgcg acagattgga
    11821 gctttggtta aagtacagaa aaattaccct gaggctattc aatcaatgat tgatgaaaac
    11881 aaaaaaatga agtcggtaat tgatgagttc aacaaaaaag aaatcatttt ggtaaagaac
    11941 cgactgaaaa cacaggttaa agaacaaaat ggaattaaat acattaccga tgttcttgat
    12001 aatatggatg ctgcagcact aaaagacatg agctatcagc ttaaatcgga aataagaggg
    12061 ttggtttgca ttctcgcagg aatatcagca aataaagtgt atttgtcagt aatgattgac
    12121 gaagaaataa tgcaagcaca caatctgaat gccggacttg ttgtgcgtga agctgccaaa
    12181 gcaatacagg gcggaggcgg tggtcagcca ttttatgcta ctgccggagg ctcaaaaaca
    12241 gagggagcaa aagatgcggt aaacattgca ctgcaaatgt gcatttcgtg agcattcctc
    12301 acccctgccc atctgtttaa ttaatgttga aaagtattgg aacgaacaga ttttttgact
    12361 ttgtaaatta aaaataatac tgttaattag catcacctta aaaacacata gaaatgaaaa
    12421 caatgaaatt cgtattactg gcagccgcag ggcttttcgc tatgagctcc tgtcagtcgc
    12481 aaaaaaaatc aactgatgaa aaaatcagtt taagcaactc aaaagattct gcctcctatg
    12541 ctatcggtat gcaaattgct aacaacttca agtcgaacgg cattgacaca gatattaaca
    12601 tggaagctgt aattgcaggg ttgcgcgaca atgggaccgg aaaaacacag ttcactattg
    12661 aaactgccga tcaggttgtt caacgttatt ttgcttcgat aagcgaaagc aaaaatgccg
    12721 gaaaagttga agaaggacag aaattccttg ctgcaaaaga aaaagagccc ggggtgaaga
    12781 aaaccgcatc aggattgctt tataaagaga taacccccgg aaccggtgct acacccaaag
    12841 ccaccgataa agtaaaaacc cattacagag gcacactgat tgatggaacc gaattcgact
    12901 catcatacaa aagaggcgaa cccgctgttt tccctgtaaa tcaggtgata aaaggttgga
    12961 ctgaagcact ccagctgatg aaagtcggtg ccaaatggga attgtacatc ccctataacc
    13021 ttgcctatgg cgaaagaggt gccggtcagt tgattggccc atacgaaacc cttattttcg
    13081 agatagaatt gataagtatc gaataatttt tgtaaaccga agcctgcttc ggtttttttt
    13141 taactaataa aaaaaaatta atcacacaac aaacaaataa atgaaaaaca aaagtctgat
    13201 atcgattaac gattacacta aagaagatta catgcgaatt ctgaatcttg ccgccgaatt
    13261 cgagaaaaat ccccggcaaa acatactgca gggatatgta attgcttcgc tgttttttga
    13321 accatccaca agaacgcggt taagtttcga aagtgctgtt cagcgacttg gcggacgcat
    13381 cattgggttc tcagaagcag gaaactcttc cgtttcgaaa ggagaatcgc tgaaagacac
    13441 cattctcacc gtttgcaatt acagcgacct aatcattatg cgacaccctg tggaagggag
    13501 tgcacgtttt gccagcgaag tggcttcggt acccattatc aatgcaggcg atggtgcaaa
    13561 ccagcatcct acccaatgcc ttctcgacct gtattctata aacaaaacgc agggaaagct
    13621 cgacaatctg agtattgcag ttgtgggtga cctaaaatac ggacgtacag tgcattcact
    13681 aattcatgcc atgattaact ttaatccggt attccacttt gtttcacctc ccacactacg
    13741 tctgcccgaa gaatacaaac agttgctgcg cgacaataaa atcccttttt acgagcatga
    13801 cgaaattacc gattgcatca gagagtccga cattatgtat gttacccgcg ttcaaaaaga
    13861 aagattctcc gatcctatgg aatatgaaaa agtaaaaaac acctatatcc ttcacaacag
    13921 tatgctcgaa ggaacacgcg ataacctgag aattttgcac ccactgccaa gggttaccga
    13981 aatcaacatt aatgttgatc caagccccaa agcatactac ttcactcagg ctcttaatgg
    14041 cgtgtacaca agaatggcaa ttatttcaat aatattaggt ttaaaataaa gaaaacatga
    14101 aacacaataa ttccgaaatg actgtaagag ccattgaaaa tggcaccgtt atcgatcata
    14161 tcccttcgaa aaacctgttt aaagtgttgt cggtaattgg cattgaacgc atggatacca
    14221 cgatatttat cggcaataac ctcgacagca aaaagcttgg gaaaaaaggt attattaaaa
    14281 tcaccgataa atttccttct ctcgacgaca tcaacaaaat tgccttactg gctcctaacg
    14341 ccgtgttcaa tatcataaga aactacgaag tagtagagaa aaaaccggtt tctttacccg
    14401 aaaaagtgca gggttttgta aaatgtatga accccaaatg catcaccaat ttcgagcagg
    14461 taccaacgca ttttcatgtt tcagtatccg atggagaaat tcagctaaag tgcaagtatt
    14521 gcgaaaaaat aacgggtgag agaaacattg aagtaatata gacggtaatg aaaaagcaca
    14581 taatcctttt ctcttcttgc gtgttgcttg cagtaggttc tttggcacaa tccaccgata
    14641 atgctgccaa cagaaacaat acgtttgctt ttacaatgct taataaactt tattccgaaa
    14701 ccaagaacac tctggttagc ccgttaagca tttcggttgc aatggcaata acctatgaag
    14761 gcgctaaggg aaagacttcg aaagaaattg ccacagtaat gggtttcgac aaaaacaaaa
    14821 aacagcacca tgtgcagttt gttgatttgg tgcagcaata ccgtgacact tcgctccatg
    14881 cattaaaaat gaacaacatt ctaatggtac agaaagacta cgcttttaat aaggatttca
    14941 ttgctgcatc attagactat agtgcgatgc tgtattttgc caatttcagg gacgatacgc
    15001 aacgggaagc tgcccgcagg ggactgaacg attgggttga aagccagacc aataatacaa
    15061 ttaaagacct gttacagccg ggcattctcg acaatctgac gcgcctggta attgtgaata
    15121 gtttgtattt taatgcaaac tgggaaatcg cattcgatgc aaaaaagacc cgtcagatga
    15181 ttttctatag catcgataaa caggttattg tgccttttat gcatgggcgc gaagacttca
    15241 actactacgc cgattcgctg atacaggttt tggagttacc ttactccggc aaacgttttt
    15301 ctatggtggt ttttcttccg aaaaacgaaa tcagtctgaa gaattttatt ggcagtttta
    15361 ctgttgaaac ttacaatttc tatatttctc aaatggcaaa acaccgtgtt gacgtgttac
    15421 tcccgagttt tacaataagc gatgagtcga atctcaggca gtttttcgaa tcactgggta
    15481 tggttaccgc ttttacaggc tatgccaatt ttaaaggaat gaatggtaaa aaagacctga
    15541 tgataaaaga tgtggttcat aaaacctata taaaagtgga tgaaacaggt accgaggctg
    15601 caggagctag tgctgttgtg atacgtgaaa aaagtgcgcc ggccgaaaaa ccggttttct
    15661 ttaacgcgaa tcgtacattt ttttatgtta tcagagataa tgcttcaaat tccatattgt
    15721 ttgcaggtag cataaacaaa ccattttaga agcagctgaa tttttgaaca agtcaaccca
    15781 aaggcacatt tgcaaataag gaaaacaatt gtatttttgg taaatattaa taacacaaat
    15841 ttttgaaaac tatgaaacaa attctcacat tactgatggt tgcccttctt tttgttgcat
    15901 gcggtaattg caaaaaatgc aaagatgacg ataagtgttg cgattccaca aaaaacgaat
    15961 gctgtaagca tcctgaaaac ttggttatgg ttgattcgtt actcactaat caggaagcat
    16021 ttctcgataa aaatatcgcc gtttgcggtg tggcaattca tgtttgcgag cattcaggca
    16081 agaatctatt cattgctact gacaccgctg ccgaagatta tctggttgcc aaagctaccg
    16141 ctgatttggg tgtttttgac aaagcgttgc tgaatgagca cgtatgtgca ttcggcactt
    16201 ttaaaaaagc cgaagttgaa gcagtggaaa cccatcacga aaaagaagtg tactattacc
    16261 tacagtgcga ttctgtaaag cagtgcaaat gtaaaggaga taacaaaaaa tgtggtgatg
    16321 ctgctaaaag cggttgcaaa gggcattccg aaaaacacaa ttgcgaaggg caataaaaca
    16381 accttctatt gcagaaagca tactcaaacg cagccgtctc cggttgcgtt ttttattgga
    16441 tgagcaacat tataagctat ctccccgcac taaccgttta atggaaaaac tgacaaagta
    16501 gtttattgtc attgtattca gccagcgtgg acgctgtctg catataagtg cttcgctacg
    16561 caatgacgtt ttcgagcccg tctttgcgcg gacgaggaac gaggacaaag caatctgtac
    16621 tggacggacg ggtattgctt acgtctccgt ggggttagat tgcttcgcta cgctcgcaat
    16681 gacgtgtggg agaatggtgc aataataaaa gcacccgcct gagtggcggg tgcttttatt
    16741 tatttgagac ccctgcgttc gaggaggtat ttttcgttgg gttcctgacc gcggaatttc
    16801 ttatactgtt ccatggccgg ggcttcgcca atttccgaaa ggcaatactt acggaatttt
    16861 gcagcgagtt ctttgttgaa caaatcgccg ctttcggcaa aggctgcaaa agcatcggca
    16921 tcgagcacgg cagcccagat gtacacatag tatccggccg aatacccgcc actgaaaatg
    16981 tgcgagaaat aggtactgcg gtagcggggc agaatttcgg gaatgaggcc gatgttgttc
    17041 atggcttcct cttcgaactt cagtacatcg tcaaccgagg caccgctttc gattaaatgc
    17101 catttcatat cgagcagcga tgcggcgaga tattccaccg tggcaaagcc ctgattgaat
    17161 agaccggagt ttaccagttt ctgaatcagt tcttcgggca tgggttcgtt ggttttattg
    17221 tgaagagcat aatggcgcaa cacttcgggt tcactggcaa agttttccat tatctgcgaa
    17281 ggcagttcca cataatcgcg gggagtaaca ccggcggtgc gtttgtagta gccatcggta
    17341 aagagtccgt gaagtccgtg accaaattcg tggaaaaggg ttgtggtttc gtcccagctc
    17401 aacagggtag gattgtcggc agtgggtttc ggaaagttgc atacaatgct tacgagggga
    17461 atttctctct taccgtcctt gtcgtatccg gcttcgcgga aaccggtaca ccaggcacca
    17521 ccctgtttgc tatcgcgcac atagtagtcc atatagagaa tgccaatgag tgttccatcg
    17581 gcttccttca cttcgaaaac ttcgacatcg ggatggtaca caggaatgtt ggtcacttta
    17641 ttaaacttaa ttccatagag ttgtgtggca acccaaaaaa caccatcgcg tacattttcg
    17701 agtttgaaat agggtttaag ttcgttttcg tcgatgttgt atttttgttt acgcagcttt
    17761 tcggcatagt agaaccaatc ccaagaggcg attttgaaat ttgcgccttc tttgttggca
    17821 atagcctgca tttctttgag ttcctgactg gctacgggaa gggctgattt ccacagtttc
    17881 atgaggaatt ggtcaacatt ttcgggtgtt tcggccatgt tttcggcaat cacatattcg
    17941 gcatagttct tataaccgag cagttgggct ttctttgccc gtttttcgac catctgggtg
    18001 atgagctttt tgttgtcgtt gtcgttacca ttgtttcccc gcataaaata gccacggtaa
    18061 attttttcgc gcaggtcgcg gttttgggca tattgcagga aaggagtcat cgacgcacgc
    18121 gaaagctgga atacccattt accttcgaga ccttggtctt ttgctgtagc ggctgcgccg
    18181 gcaataacat cggcgggtaa accagccaga tcggcttctt tatcaacaac cagtttgaag
    18241 ttcacattgg tttcggccag gaggttatcg ccatattgca ggtcgagcat cgagagctcg
    18301 ttgttgagcg tacgcagggt ttccttgtcg gcagccgaaa gggcagcacc actgcggata
    18361 aagtcgttgt agtatttttc ggttaccctg atgcggtcgg gactgagacc ggcttcgagg
    18421 cgggaatcgt aaacggcttt cacgcgtttg aacaaatctt cgttgagcat gatgttgttc
    18481 tggtggtcgg taaccagtgg tgttacttcc ttggcaatag cctgcagcga gtcgctcgat
    18541 actgccgaaa gaaggttgaa gaacaccgag cttactgtgg ttagcatttc gcccgactgg
    18601 tcgagggcca ggatggtgtt ttcgaaatct ggtgcttcct tgttgtcggt aatagccttg
    18661 atttcctgct cgtgtctttt gataccttct ttaaaagccg gcaaatagtg ttcgtttttg
    18721 attttatcga acgccggaac accataaggc gtgtcgaaag cggtgaagaa cgggttttcg
    18781 ggttgcatat cggtcgcttt tttgttttta catgcgcctg ccagcagtat cagcggcaga
    18841 caaatcagaa tgagtcgttt catattgttt agtttatatt gtttggttca catcattaat
    18901 tttcgttgtc cggatccata ccaattattg taatgggcac aggcttcaca aaattgagat
    18961 tttttttgaa ataaagttcc ttgttccggt tctgatttgt taaaatctga cagatttaac
    19021 tgaattttaa gttttcagcg cttcagattc gatttattga gtttgtcgtc aagcagcatc
    19081 aacttttaac aattaaatca tattgataat cagatggtta taaccgaaat gattctaaag
    19141 agacaagtcg gtagcaaaaa gcggttgcaa agggcacgat accagaagca caacagcaaa
    19201 ggacaataaa aacaacatac tattgcagaa agcatactca tacgcagctg tctccggttg
    19261 cgttttttta ttgaatcttc aacagcaaat cagtaattat ccgcttgtga tcgaatgcca
    19321 gttccggcaa atcgtttaca tcaaaccatc ccgcttcaat agcatcatca ttaccggtaa
    19381 ctgttactgc ttctggaata atggaataaa aaacaacagc gatattgcgc ccccttggat
    19441 ctctgtcaac attgccatag gctttaaatt gttccaggtt tgcaagcgat aaacctgttt
    19501 cttcgagcaa ctctctggcc gcagcaattt cgagggattc gttcatatcg acaaagccgc
    19561 ccggcaatgc ccatcttcct gcaaaaggtt cattttttcg tctgatcagc agaactgaat
    19621 gcgaaaccga tgtagcaatt accaccatgt cgacagttac catgggtctg gggtattgat
    19681 aggagtattt catttgttaa aatttatttt aattagtcac aaggaatctc gcataattaa
    19741 tattcatctc tgtttgtgtt atttttatca tactcgattt cataagatca tgcgagttat
    19801 attaacgatt gtcaggaata ttttttaggc aaaaaacagt ctctttttcg ttgatatcca
    19861 tttcaataag attcatcaac aaaagattga caaggaaatt ttcggtactg aatttattca
    19921 tattggttag ttgcctgcat acatcgaagg taatcgcacc attcctgttc agataattca
    19981 ggagcatttc cacattgcgg tcaagcgcaa tggctgtaat attaccagaa ttcagtcgct
    20041 ttttatattc aagcagaatt cggtttgcag gcagattttg gtcgttacgg cgaatataca
    20101 ccagccattt attattttcg tctggtgcat agtgtggaac aaatccggtg gacgggataa
    20161 tcacttcgaa aacgtttttt ccattacacc gatgtgtttt acaacggaaa ggcacttcgg
    20221 gtttgcaaaa cgtctgcgca gctttttcaa tcatataata ttcttcgtcg gatgccacac
    20281 ctgagatttt accgttatct ttaacgccaa gcagtagggt gcctccattg gtgttggcaa
    20341 aagctgtcag tgtacgggca attttatgtg catcgttcac acagtattta aaatcgagct
    20401 gctgcccctc cccttgttct atcagacgtc ttatgtccac ctgcaatttt tgcctgtaaa
    20461 gataggaaga tcaaggcaat tatcccttaa gtatattatt tgttacttct tttttcgatt
    20521 tcttgcttca attcgttggc tttcatatag tcttcttctt gtaccgcttt agccttcatc
    20581 tcctgcagtt gctcaatgct gtaatcatcg tacttgcctt tcagggtttt gaaatcgagc
    20641 aggttgccgg tgattaccaa cggaaaatcc tgcccatagc tataggcggt ggggtgttgc
    20701 ggtgttttgg tgggtgtgta ggttaccgat aattccggca cctgtttctc ggtgtagttt
    20761 gatatgtcct tcaccgtaag actcttacct tcggggtaca ggccatcgcc tccggtcatg
    20821 gctttcagca gggagtaggt aaaaacccca tgacctatct caggaatttc ttttgccaac
    20881 tggttggcgg tgctggcaaa caggaagtga gtacctgtac tgcgtgccaa tacggctata
    20941 gcccgctcct cttcaggtcc gctgcgatat gccagtaaat ctcccgctgc tccgctgttg
    21001 caggcatcaa tcaccaaaaa ctgcttctca gcctgtatat cgcgcgagaa aaacaacagt
    21061 tcgctgttcg aaatggcttt gcctgtgagc tgttccttgc tgtaaatatt tgttacatca
    21121 gcattaatga aatagaagtt ggttcgtcct tcttctgcaa tggctgctcc gtgacctgca
    21181 tagtaaaaca caaacacatc ccatggtttg gctttcgact gcacctcatt gagaattttt
    21241 gtaatattgg ctttggtagc atcgcggtcg gtgatgttgt aggtatagat attcccgaac
    21301 aaggtctttg cccctgtttc tattgcctgc gaaaaactga gggcatcgct taccgcaaaa
    21361 ttcaggtggt attgtgggtt ctcgtattcg ttgataccaa tggaaaccag atacaggtcg
    21421 gctgtctctg tgatagtacc cgaaaaattc atcctaatgg tgtcgcatat ttcggtgcga
    21481 tcgctgttga ataccgatac ggtaatggtg ttgattccgg gcatgagcgg aacatcgaag
    21541 ctccgcgaaa tgctttcacc gggcttgttc aaaccaatta tttccgattt caccaacttg
    21601 ttgttgcgat acacacgaac ctcttccacc ccaccaccat tgtcggtaaa gctggctttt
    21661 agtttgagca ggttcgcatt ggtgggcacc ggcatattga tactgcgata atcgccttgt
    21721 gccggttcgg tgatctttac ccccgaattg ggtttgcgct tgttaaaatc aaccttcgat
    21781 tgctctatgc tctcacccct gagaatgcgc tgcaacagat tggggcggta gaactgctcg
    21841 taatatgctt ccagcggaat gatatccgtt ccctgcacat aatacaggta tctgagcgct
    21901 tcgggagtac catcgaaata tccttctgga gttaccacca ccgttaaact atcgcctacc
    21961 ggataaatgg atgctagttg cttttgggtg gcgatgtccc aatagatgat aaccccatct
    22021 tcggatgcag atatcacttt tcggtcgtta tcaaaaaact gcacacgcga cacatgtttt
    22081 gtattacttt taaaactgat tgcagtacct tttgaaatat cccagatttt aattaggcca
    22141 tcatttgcac atgaggcaat cattgaacca tcattcgaaa agcagatatc atttatcttg
    22201 tcggcatgcc ccgacagttt tttaatgatt ttaaagccgt tgaaatcgta gatataaatg
    22261 actttgccaa tttcatcaga gaatgctatc aactgctttt ttaaatttac acaggaagaa
    22321 taaac
//
LOCUS       DAOOQP010000039        22309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2831_length_22309_cov_6.912016, whole genome shotgun sequence.
ACCESSION   DAOOQP010000039 DAOOQP010000000
VERSION     DAOOQP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2831_length_22309_cov_6.912016"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLK75_05850"
     CDS             complement(<1..448)
                     /locus_tag="PLK75_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY38807.1"
                     /translation="MKALLGLFALLLGSLLLHAQNNNIIYIDEVEVVAAKQSNLLKNC
                     PEIIRVVSRAEIDKFNYGSTGEILQHIAGVNIETGTGSGQPKRSTASLNGYPAQYTLV
                     LVDGNRVLSDHVHTGQNLDLIPTESIERIEVITGAASAQYGSDAMAG"
     gene            complement(652..2277)
                     /gene="pruA"
                     /locus_tag="PLK75_05855"
     CDS             complement(652..2277)
                     /gene="pruA"
                     /locus_tag="PLK75_05855"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOY38808.1"
                     /translation="MNNAIFSFESPVNEKIFLYEKGSVEREEMLKELERQENKVLEIP
                     LIIGGEEIKTGNTGTVVMPHKHQKVIAIYHKAGEAEAKMAIESALKAKEQWQAISWVE
                     RASIMLRAAELLSKKYRYVINAATMLGQGKSVMQAEIDAACEVIDYLRYNVYFASRIY
                     HEQPKSNFDQLNRLEYRPLEGFVYTITPFNFTAIASNLNMSVALLGNTTVWKPATTAL
                     LSNYYLMQIFKEAGLPDGVINFIPGEGSVLSKVILAHKDLAGIHFTGSNATFNSLWKQ
                     VSQNIEGYTSYPKLVGETGGKDFVMVHNSANPREVATAIVRGAFEYQGQKCSAASRAY
                     IPVSLWPEVKNHIVSMLKEVTVGDVNDFSHFMNAVIDEKAYDSIMGYINYAKSSPECE
                     IIAGGAGDKSVGYFIEPTVIVTSNPKFRTMQEEIFGPVMTIYVYEDSKYSETLKLCDQ
                     TSPYALTGAIFANDKYAMVQACQTLRYAAGNFYVNDKPTGAMVGLQPFGGSRGSGTND
                     KAGGHLNLLRWTSPRTIKETFLPATDFEYPYMR"
     gene            complement(2506..3036)
                     /locus_tag="PLK75_05860"
     CDS             complement(2506..3036)
                     /locus_tag="PLK75_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY38809.1"
                     /translation="MIEIQKIALVNENDEITGYGEKFDVHRRGLLHRAFSVQIIHPEK
                     GFLLQKRALRKYHSAGLWSNTCCSHLPENFEMETAVRQRLMLEMGIDCKPVYVGKFAY
                     SSVFRNGFTENEIDHVYVGIWEGVPEMHPDEADSFTWIFPDMLKINLAAYPELYTVWL
                     PFVFDTIRNSENAVLF"
     gene            complement(3029..4330)
                     /locus_tag="PLK75_05865"
     CDS             complement(3029..4330)
                     /locus_tag="PLK75_05865"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOY38810.1"
                     /translation="MNSTPLNIVWFRRDLRIDDNCSLYNAMNCGYKVLCIFIFDTQIL
                     ESLVSGPDRRVTFILKALTNLKLKLQLLGSDLIVFHGKPIEVWQKILSEYSVNAVYYS
                     VDYERYSIYRDREISSFLMSRGIQSYPFHDHVILRPDQVLKNDETPYTVFTPYSKKWR
                     EFLHSCPIKTFPSESIAQEALLPVNPVPLPTIQQLGFIDLGTEIAQCNIRNEIIESYH
                     LTRDNPAIDGTSRLSAALRFGTISIRKLALYAQQHNSVFLNELIWRDFYQMILFYFPH
                     CEFSAFKPAYEGIEWLNKESEFERWCNGETGYPMVDAAMHQLNATGWMHNRCRMITAS
                     FLCKHLLVDWRWGETYFASKLIDFETASNNGGWQWSSGSGCDAAPYFRVFNPMLQQRK
                     YDEKGDYVNTWNGNGGIKPIVEHEFAVKRCTYTYKKALQRD"
     gene            4382..5047
                     /locus_tag="PLK75_05870"
     CDS             4382..5047
                     /locus_tag="PLK75_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOY38811.1"
                     /translation="MLENFIDIHTHKALGEHAIINLFHNQIPTDSRRFSMALHPWHIE
                     TLSFESQLCKLEHHLQSDRVIAIGECGLDAFAIAPQDIQEEVFRWHIRQSEELGKPLI
                     IHCVKSFNRLIEIKKELSPSQAWIIHGFCSGNGVLQQLLHHGFFISIGTVLLTEQFKL
                     KAYLKKIPVSRLFFESDESNTRVRELYQFASGFLNVPIDELISTISDNFANVFKISSR
                     ELE"
     gene            5034..5750
                     /locus_tag="PLK75_05875"
     CDS             5034..5750
                     /locus_tag="PLK75_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOY38812.1"
                     /translation="MNWNERTILLLGSEKVKVLENSHVLVAGLGGVGAYVAEMLCRAG
                     VGKLSLIDADVVNPSNLNRQLIALRSNIGKAKPTVMEQRLKEINPDIELLISEVFLKD
                     EKIDEALSIPYDFVADAIDTLSPKINFIKTCLENKLPLISSMGAGGKTDPEKVKIDDI
                     SKSYNCNLARILRKRLHRLGIREGFRVVYSSEKADKSSVIEEESLNKKSNVGTISYMP
                     AIFGCFMAAEIIRSLTEKPE"
     gene            complement(5763..7379)
                     /locus_tag="PLK75_05880"
     CDS             complement(5763..7379)
                     /locus_tag="PLK75_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY38813.1"
                     /translation="MIKKISLFTCCTISLFLMLCNPVYGGARPAMKSPALTFIISVGI
                     NEYNSQEFSKLLYCESDAAAFINRAKTDSSLVIAGSYLLLGKEATKESIRNAFLEIST
                     KAQAGDILIFYAAAYGKYGSIVLGNSEEITSEELFGWSQSIYAVNQLYYLDISYGDKF
                     IGQLQRFFVNRPDESAISQLNRIVLSTPMAFEAASYGGGLFTMSYVKNKGIRLSDVFS
                     SSHRSSTHFLQSLYFFSDSIGEKRMELEYFSEKELYKSLIVDEVTKSITINYQNDSLE
                     NDSKMHIRKGETLCFIMGCNNFDNFNKLNNAVSDSREIKDLLGANYFTQIVYLENPTC
                     SDFKIKLKEIRSKYTFDEGSQFLLYISSHGEKNELDEGCLIFKDSDFDGNFLSNTFLL
                     SSLKRFVGQLDCTNSLILLDICHSGTMFDEELCVKPNAIEIPQTNIIFNEKFSEKSIV
                     YKNFLNQETKIFIGSSNDQIASDGTQSHSPFAAVIINFLSKNLFPINDSYYLQQEIRN
                     GIMKAGAISIPLFCMYGGCKDDGRFLFIKK"
     gene            complement(7513..8424)
                     /locus_tag="PLK75_05885"
     CDS             complement(7513..8424)
                     /locus_tag="PLK75_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOY38814.1"
                     /translation="MLNEKGKGMLVGLAIGDALGVTLEFTARGSFEPISNIIGGGPFS
                     LQAGKWTDDTSMALCLADSLIEKGGFDAHDQMEKYLDWYRNGYNSSERGCFDIGLTTR
                     NALRTFEKTGEVYCGSENPNTAGNGSIMRLAPVAIKYHNDTDLLMEYAALSSKTTHAA
                     PEAVQSCMLLGLYLQAFLHDKDKNFIFSAEFIQGAQKKIPDMLPNISYILNGSYIHKN
                     EAQINSSGYCIATLESALWAFAGTDNFRDCLLKAVNLGNDSDTVGAVTGQIAGAYYGY
                     SEIPQSWKEILYLENEIAERAEILLID"
     gene            8544..9098
                     /locus_tag="PLK75_05890"
     CDS             8544..9098
                     /locus_tag="PLK75_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38815.1"
                     /translation="MSLKQSILLIIIIFFVANYSNAQYSRMYDLQNKQITQDSLHLDS
                     IVRINFSYIPWDIHDYGAFTENEIRYFQGKTSYFSIVNQDSIKYFVNSFNILNLSIFE
                     KENNKKKRKPEAQILFDPRMVIDFIYKSGRIETLLLDRNGNIYYDANKYFTNIKFNVI
                     VIETVPPAERKWWEPPFTKPEEDE"
     gene            9091..9243
                     /locus_tag="PLK75_05895"
     CDS             9091..9243
                     /locus_tag="PLK75_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOY38816.1"
                     /translation="MSRDVLNLEAKCYDSDASIWLSFDPMADQRSWVSPYSYCQNSPV
                     MRVDPN"
     gene            9397..10437
                     /locus_tag="PLK75_05900"
     CDS             9397..10437
                     /locus_tag="PLK75_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOY38817.1"
                     /translation="MKEIKSNIIGSLVFKDVCQLIEQTKSGIARVVNSEITMLYWYIG
                     GRINAEILENKRAEYGKQIVSQLATLLTQNYGKEFEIRNLRRMIQFSILFSNKEKVSQ
                     AATKLTWSHFIELLTIKDDLQREFYLLMAIHEGWGRDTLRKQIASMLFERTAISGKPE
                     DFIKKGLSDLSENNKISPELVFKSPYFLNFTGLKGYFSEKTLEDILIGELERFILELG
                     NGFSFVERQKRMIIDGEDFYLDLLFYHRKLKRLIAIELKITKFKAAYKGQMELYLEWL
                     NKYERQDGEESPLGLILCTNGGTEQIELLKLDEAGIKVAQYYTELPDKKLLEEQLRKS
                     IEVAKMRMSENQ"
     gene            10541..11422
                     /locus_tag="PLK75_05905"
     CDS             10541..11422
                     /locus_tag="PLK75_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOY38818.1"
                     /translation="MGRAYQHLQYFPFGELFVSQRNSSFDSRYKFSAKELDNETGYSY
                     FGARYYDSDLSSWLSVDPMSDKYPGLSPYVFCSNNPANRIDRDGMKDTTFVAGKDKQV
                     SPKTGTATPIRNPDGTINTNAYNCHSYAWESSQGDPTDPRNTNLVQNGVTKWDNNPDN
                     NMSSYTQLGANDPNQKGDRVIYYTDANGNGRYDMGENIEHSAIVQTVDKNGNTATVIG
                     KMGQAGISENHPTAPGYYVIDAKGNPTSRAYFRSPSSSTVSSPELSFPDLKFDPYVMQ
                     RNATYVAPPIIIPIRKE"
     gene            11425..11991
                     /locus_tag="PLK75_05910"
     CDS             11425..11991
                     /locus_tag="PLK75_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38819.1"
                     /translation="MKISKCLLLLFMGLFSCNGPNACDCSLSEKSLSSITYEEALFLN
                     KKIIPCLIDSIDILEISFIGFQNPITSDIGNYHLNQKGIRFAYLVDYILSKDSVETVN
                     KTWNDEEDWMHWSELTKPYRIYNNGIIVKQDSNNKPILEPLTPKDMVNIKKMYFDWWK
                     QNKYKPIETLREEFRKGNTILQLPYVWI"
     gene            11982..12803
                     /locus_tag="PLK75_05915"
     CDS             11982..12803
                     /locus_tag="PLK75_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOY38820.1"
                     /translation="MDLVRKHCKWLLYHSRYKFSAKELDNETGYSYFGARYYDSDLSS
                     WLSVDPMASERSWVSPYSYCQNNPMNRVDPTGALDIIDDYYFDQNGNLVDRVETETPD
                     RFFVQDGFEIDPNDLNMVPEPSFKEITMNSDIGIIARTTYAEMRSGDNNAKSIVAESI
                     VNRHSLPDGTYEKANGTYSGIVNRFYDATKIGDPNYDVFTNPQNYYNKSTKENTAWTN
                     SVGAAVKAYYGNSNVGQGVIFYNSQSSTIYDKNPGMQKINLSISVQGIKGLWKLK"
     gene            12812..13438
                     /locus_tag="PLK75_05920"
     CDS             12812..13438
                     /locus_tag="PLK75_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38821.1"
                     /translation="MKTLIFLNILVIFMGCSWSNKGYEFNNKGLFIEIADTTKPVNLP
                     NGSYYKLIYINEFQYKIEWGNRQIKNISKDTFDISGSGPLYLLESSNDFIILQQGCGM
                     NCKYSVVLPLSSKVEEQTYIFTLALDTKDNLIAYLPEDQKNFIVVKNYLTGKVMEIEE
                     DDLCPAASSGDCIDTCYFDKSCLIIKWQGSKWQSTQPDTKERKIKINI"
     gene            complement(13515..13853)
                     /locus_tag="PLK75_05925"
     CDS             complement(13515..13853)
                     /locus_tag="PLK75_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY38822.1"
                     /translation="MLYIGDKIIQLRKLRNLSQTDLAQKVEASRTIIGNYERNENTPS
                     IEMIIKISKVFNVSVDYIIGEGVLSQYDKEVLKRIDDIEKLDDETKNKMFFLIDNIIQ
                     NFKTKQAFAK"
     gene            13843..14328
                     /locus_tag="PLK75_05930"
     CDS             13843..14328
                     /locus_tag="PLK75_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38823.1"
                     /translation="MYNISHLTFDFRHLTWKCKLLCLLVVAMLFSSCSAEKRLHRLVA
                     LHPELATKDTIRIQDTTLIPEVRIDTLVHFSRLKDTVTIEKEKLTVRIHQVRDTVYIE
                     AVQKPDTIVHVKEIPVEKIIHENPYAKEKQQNAGWVQALALILTCGIVYLLIKLDKKE
                     L"
     gene            complement(14453..14776)
                     /locus_tag="PLK75_05935"
     CDS             complement(14453..14776)
                     /locus_tag="PLK75_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38824.1"
                     /translation="MNKQMITPNQMCRQNIIGVKVIPENRQFTDLVHGFTTRQIKRTN
                     YLKVCWMCGKVYESKRYDSYACSPQCGSNIAYALKRGHRPPANMDRLTKEKRVKDLLD
                     RFGYE"
     gene            15390..15764
                     /locus_tag="PLK75_05940"
     CDS             15390..15764
                     /locus_tag="PLK75_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38825.1"
                     /translation="MKKTLIFVEIAFLFAIFIFYASACNKLPQCENPQAATIVYFGDD
                     YDTCACDEEVYIVLDDMPNDTFVIGNKIQNKYKRNGLNVEVEWDWANHLIGPQMGDEP
                     YPSNWCGPKFPIRINILCINKL"
     gene            15904..16944
                     /locus_tag="PLK75_05945"
     CDS             15904..16944
                     /locus_tag="PLK75_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38826.1"
                     /translation="MRYLSNLLLLIATVCSGNAGFSQHGTKDWEWEWAPVGAVWLYET
                     ANMGTNNIYAQYWYVRSAKDTLFHGFNCRKLEVERHYWLGFEPVKMPDRFTYQDGGDI
                     YFYNADINEFVLSFSYDIQHGDTVTWQMPVFEHCVNSYYLDTILNWDVGYLSGAGTAY
                     TPYSVFMYSNVPISSYRIRRVENNCFAGELSTNTNYSHGRYLDFIGTGSDLYELTILE
                     EMQTSCQCYYDGSVIINLAIVSINNDTIIYAEDSCLNYYNKFNGISSHIGKKSLRVYP
                     NPFTDLINVDLPAIEGNYDISVYNLMGQLLYSDKTRSNNTPLHLSRLVNGTYLLQIRT
                     NDNVFNQRIIKN"
     gene            17205..18350
                     /locus_tag="PLK75_05950"
     CDS             17205..18350
                     /locus_tag="PLK75_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY38827.1"
                     /translation="MYLRRQIFEGIGKESAFLWGARQTGKTTLLKMMFPDSMYIDLLL
                     NSEFLRFSQNSNLLREIVEANPTEKPIIIDEIQRIPELLNEVHWLIVNKGTQFILSGS
                     SPRKIIRSGVNLLGGRALRYELYPLVYPEIHNFDLNKILNRGALPRHYLSENPKRLLG
                     AYIGSYLRDEIISEARLRNVSAFTKFLESAAFSNAELVNYSNIATDCGVSSVTVKEYF
                     GILEDTLIGRFVPAFTLRAKRRVVASPKFYFFDIAVANYLLNQNHLAEKSEAYGKALE
                     QFIYLELYAHSRYSGLHYNISYWRTATQIEVDFILGDGEIAIEVKSNENIQAKHVKGL
                     RQFASEYDVKRLIVVSKDPYYRKIDNIEIMPVEKFLQELWSNNVIKG"
     gene            complement(18388..18786)
                     /gene="ssrA"
                     /locus_tag="PLK75_05955"
     tmRNA           complement(18388..18786)
                     /gene="ssrA"
                     /locus_tag="PLK75_05955"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18913..20130
                     /locus_tag="PLK75_05960"
     CDS             18913..20130
                     /locus_tag="PLK75_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY38828.1"
                     /translation="MATKIRIGILKETKTPPDRRVVLTPENVLQAMKHFPEAEFVVQK
                     STLRCFTDNEYLEKGIAMADDMSDCDVLMGVKEVHIPELIPNKQYHFFSHTHKKQSYN
                     RPLLQALVKNHITMVDHECLTYENGARVVAFGRWAGIVGAYNAMLGWGLRTGDYQLKR
                     AVDCFDRNEMIGELKHVKLAKNLKILITGKGRVGMGAMETLSGLKLKMVTAKEFLTQQ
                     FNEPVICQIDADEYVEHKDGGWKDFPYFFKHPAEFVSTFSPFSKVTDIWIPCHFWDQN
                     SPAFLKPADYHAPDFRIKLIADVSCDLIDPIPSTLRASTIKEPFYGYNPKTEKECAAF
                     DKNNVTVMAVDNLPGELPRDASGEFGARFIEEVIPAIVGKDEKGIIMRGTICSRGKLT
                     ERYAYLEDFLNGK"
     gene            complement(20256..20909)
                     /gene="rpe"
                     /locus_tag="PLK75_05965"
     CDS             complement(20256..20909)
                     /gene="rpe"
                     /locus_tag="PLK75_05965"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOY38829.1"
                     /translation="MSVIVAPSMLSANFLNLSSDIDMINHSKADWFHLDVMDGVFVPN
                     LSFGLPVVKAIKKEAHKPLDVHLMIVSPEKYIQAFRDAGADILTVHVEACTHLHRTIQ
                     EIKNAGMKAGVSLNPHTPVQMLENVIADIDLVLVMSVNPGFGGQSFIQSTYRKLELLS
                     ELKQFDNPDLIIEVDGGVTLENAPELVKAGVNALVAGNTVFASKNPVETIRQLKLVG"
     gene            complement(20988..21854)
                     /locus_tag="PLK75_05970"
     CDS             complement(20988..21854)
                     /locus_tag="PLK75_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOY38830.1"
                     /translation="MRQLKIVKQVTNRDTPAFEKYLQEIGRKVMITPEEEIELARKIK
                     MGDRAALEKLVTSNLRFVVSVAKQYQNQGLSLQDLVNEGNLGLIKAALRFDETRGFKF
                     ISYAVWWIRQSILHSLAEQGRMVRLPLNKIGAINKIHTISSKLEQQFQREPTAEEIAE
                     EIEMTAYEIKDNLKNNVRHVSMDAPVGSDPDDGTLADLIDINTDNPPDYSLLKESLMR
                     EINSTLSVLGKRESEILKMYYGLGGEHAKTLDEIAEYFCLTRERVRQIKDASIRRLKQ
                     STKHKKLKYYNL"
     gene            22091..>22309
                     /locus_tag="PLK75_05975"
     CDS             22091..>22309
                     /locus_tag="PLK75_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOY38831.1"
                     /translation="MYDINQLNEKLLPELKLIARDLGLVRTDMLKKEELVYKILDQQA
                     INASAIPDEKKQRPPRQRRRAEKITINTP"
BASE COUNT         6991 a         4359 c         4324 g         6635 t
ORIGIN      
        1 caccggccat tgcatcgctg ccatattgcg ccgaagcggc tccagtaata acctcgatgc
       61 gctcgatact ttcggtgggt atcaggtcga ggttctgtcc ggtatgcaca tggtcagaca
      121 acaccctgtt gccatcgacc agtaccagcg tatattgtgc gggatatccg tttaaactgg
      181 ccgttgaccg tttcggttgt cctgacccgg ttccggtttc gatgtttaca ccggcaatat
      241 gctgcaatat ttcgccggtt gagccgtaat taaatttatc gatttcggca cggcttacaa
      301 ccctgataat ttcggggcaa ttttttaaca ggtttgattg cttcgctgcg actacttcaa
      361 cttcatcaat gtaaataata ttattgttct gagcgtgcag caacaacgag ccaagtaata
      421 gcgcaaacaa tcccaaaagt gctttcatgt ttttttttca aaattagaaa acattcgggt
      481 caataatgta aaattatttg agacttaaat ttaccaatta ttaatctaaa gaaccccaga
      541 aagatatctg taagcctgat tgcacaatca gaaactcaga caagttttct gtctgaaaaa
      601 atgacttatc ctggctccta ttgtgttgag caactacatt atagctttgt actacctcat
      661 atagggatat tcaaaatcgg ttgcaggcaa aaatgtttcc ttaattgtac gcggacttgt
      721 ccaccgcagc aaattcaaat gccccccggc cttatcgttg gtacccgaac cccgcgatcc
      781 gccaaacggc tgaagcccaa ccatagcgcc cgtgggtttg tcgtttacat agaaattacc
      841 tgctgcgtag cgtaaggtct gacaagcctg caccatggcg tatttgtcgt tagcaaaaat
      901 ggcgcctgta agtgcatacg gactggtttg gtcgcacagc ttcagggttt cactgtattt
      961 cgaatcctca taaacgtaaa tagtcataac cgggccgaaa atttcttcct gcatggttct
     1021 aaatttcgga ttggaggtaa ctatcaccgt gggctcaata aaataaccca ctgatttatc
     1081 gcctgcacca cctgcaatta tttcgcattc cggtgaagat ttggcgtagt tgatataacc
     1141 cataattgag tcgtaagctt tctcgtcgat tactgcattc ataaaatgcg aaaagtcgtt
     1201 cacatcaccc accgtcactt ctttcagcat cgaaacaatg tgattcttta cctccggcca
     1261 aagagataca ggaatataag ccctggatgc cgccgaacat ttttgtccct gatactcaaa
     1321 agccccccgc acaatggcag tagccacttc gcgcgggttt gccgaattat gtaccataac
     1381 aaagtctttg cctccggttt cgcccacgag tttcggatac gatgtatatc cttcgatatt
     1441 ttgcgaaacc tgtttccaca aactgttgaa ggtagcatta gaacctgtaa agtggattcc
     1501 ggccagatct ttatgtgcca gaattacttt gctcagcacc gatccctcgc ctggaatgaa
     1561 attaattaca ccatcgggta aaccggcttc cttaaaaatc tgcatcagat aataattcga
     1621 tagtaaggca gtggttgcgg gtttccacac ggttgtgttg cccaacagag ccaccgacat
     1681 attcaggtta gatgctatag ccgtaaaatt aaaaggtgtt atggtgtaca caaacccctc
     1741 gagcggtctg tattccaacc tgttcaactg atcaaaattc gattttggct gttcatgata
     1801 aatgcggctg gcaaaataga cattatagcg caagtagtca atcacctcac aggcagcgtc
     1861 aatttcggcc tgcataacgc tctttccctg acccagcatg gtggctgcgt ttataacgta
     1921 acgatatttt ttcgataaaa gttcggcggc acgtaacata atcgatgccc tttcaaccca
     1981 tgagattgcc tgccattgct cctttgcctt tagggcactt tcgattgcca tttttgcctc
     2041 ggcttcgcct gccttatggt agattgcgat tactttttga tgcttatgcg gcattaccac
     2101 tgtaccggta tttccggtct taatttcttc cccaccaatt attaatggta tctcaagcac
     2161 tttgttttcc tgacgctcca gctccttaag catttcctca cgctctacac tgcctttttc
     2221 atacaaaaaa atcttttcgt ttacaggact ttcaaatgag aatattgcat tgttcatagt
     2281 ttgtaaatat ttgagtaaat aaaaatttcg agttgcaata atatgcattt cgtatcattt
     2341 atacaatgac ttttagcata acagcattta tgcacttctt gtttatattg aacagattaa
     2401 tcctttgctc cgaaataagt cgaacaattt tcaaaatcaa tacaactaat tattaatcaa
     2461 ttatatagat tattaacaca tcgagtatcc cgaaagaaaa acattttaaa acaaaacagc
     2521 attttcacta tttctgatag tatcgaaaac gaatggcagc cataccgtat atagttccgg
     2581 gtaggcagcc agattgattt tcaacatatc gggaaaaatc catgtaaatg aatcggcttc
     2641 atcagggtgc atttcaggga caccttccca tataccaaca tatacatgat caatttcgtt
     2701 ttcggtaaaa ccatttctga aaaccgatga atatgcgaat ttcccgacgt aaacaggttt
     2761 acagtcaatc cccatttcaa gcatcaagcg ctgccggact gctgtttcca tttcgaaatt
     2821 ttcgggaagg tggctgcaac atgtatttga ccacaatccg gcggaatgat atttacgcaa
     2881 cgcacgtttt tgcaataaaa agcctttttc agggtgtatt atttgtaccg aaaaagctct
     2941 gtgcagcagg cctctccgat gtacatcgaa tttttcacca tatccggtaa tttcatcatt
     3001 ttcgtttacc agtgctattt tttgaatttc aatcacgctg cagtgctttt ttataagtat
     3061 aagtacatct ttttaccgca aactcatgct caacaattgg tttaattccg ccatttccgt
     3121 tccaggtgtt tacatagtcg cctttctcat catatttcct ttgctgaagc atggggttaa
     3181 acacacgaaa atagggcgca gcatcgcaac cacttcccga cgaccattgc catcctccat
     3241 tgttgctggc agtttcgaaa tcgatcagtt tcgaagcaaa atacgtttct ccccaacgcc
     3301 aatcaaccaa caaatgctta cacaaaaacg aagccgttat cattctgcac cggttgtgca
     3361 tccagcctgt cgcgttcaac tgatgcatgg ctgcatccac catcgggtat ccggtttctc
     3421 cattgcacca gcgttcaaat tcggactcct tgttcaacca ttcaataccc tcataggcag
     3481 gtttaaaagc actgaattcg caatgcggaa aataaaacag tatcatttga tagaaatcgc
     3541 gccatatcaa ctcatttaaa aagacgctgt tatgctgctg cgcatacaat gccagtttcc
     3601 ggatactgat agttccaaaa cgcagtgcag ccgacaaacg cgaagttccg tcgatagcag
     3661 ggttatcgcg ggttaaatga tagctttcaa tgatttcgtt tcgaatgttg cattgggcta
     3721 tttctgtgcc caaatctata aaacccaatt gctgtattgt tggtaatgga acggggttaa
     3781 cgggcaacaa tgcttcctgt gctatgcttt ccgatggaaa tgttttaatc gggcaactat
     3841 gcagaaattc tctccatttt ttcgaatacg gggtaaaaac tgtatacggg gtttcatcgt
     3901 ttttaagcac ctggtcagga cgcaaaatta catggtcatg aaacggataa ctttgaatac
     3961 ctctcgacat taaaaaagaa gagatttctc gatcgcggta aatactgtat cgctcataat
     4021 cgacactata atataccgca tttaccgaat attccgaaag aattttttgc catacctcaa
     4081 taggttttcc atgaaaaaca ataagatcag aacccagtag ctgtaatttt aatttcagat
     4141 tggttaatgc cttcagtata aaagtcaccc gtctatcagg tcctgaaacg agactttcaa
     4201 gaatctgagt atcgaaaata aaaatgcaca gcaccttgta cccgcaattc atagcgttat
     4261 ataaactgca gttatcgtcg attcgtaaat ctctccgaaa ccagacgatg tttagcgggg
     4321 tcgaattcat gaattagtat tatcctgttg aagatacatg ttttttgcat aagtttacac
     4381 catgttagaa aattttatcg acatacacac tcacaaggca ctgggagaac atgccatcat
     4441 aaacctgttt cacaaccaaa tacctactga ttctcggcgc ttttcaatgg cgcttcatcc
     4501 ctggcatatc gaaaccctga gctttgaaag tcaactctgt aaattagaac accatcttca
     4561 atcagatcgc gtaattgcga ttggagaatg cggacttgat gcttttgcta ttgcacctca
     4621 ggatattcag gaagaagttt tccggtggca tatcaggcag tcagaagaac tcgggaagcc
     4681 cctgatcatt cattgcgtaa aatcgtttaa ccgtttgata gagattaaaa aagagctttc
     4741 gccttctcaa gcctggatta ttcatggatt ctgttccggg aacggggtac tgcagcaatt
     4801 gctgcatcac gggtttttca tctccatagg aaccgtattg cttacagaac agttcaaatt
     4861 aaaagcatac ctaaaaaaaa tccccgtttc gcgattgttt ttcgaaagcg atgaaagcaa
     4921 cacccgggtc agagaattgt atcagtttgc ttcaggattt ctgaatgtac cgattgatga
     4981 attaatttca acaatttctg ataattttgc aaacgtcttt aaaatttcgt cgcgtgaact
     5041 ggaatgagcg aaccatattg ttactgggaa gtgaaaaagt gaaagtactt gaaaattccc
     5101 acgtactggt tgccggactc ggcggcgtag gagcctatgt tgctgaaatg ctctgccgcg
     5161 caggagtggg gaaactcagt ctgatagatg ccgatgtggt aaacccttca aacctcaacc
     5221 gtcagctgat tgctctcaga agcaacattg gcaaagcgaa accaacagta atggagcagc
     5281 gcctgaaaga aattaatccg gatattgaac tcttgatttc ggaagtattt ctgaaagatg
     5341 aaaaaattga tgaggcactt tcaattcctt acgattttgt ggctgatgcc atcgatacac
     5401 tgtcgccaaa aattaatttc atcaaaacct gcctcgaaaa caaactccct cttataagtt
     5461 caatgggagc cggtggtaaa actgatcccg aaaaagtgaa gatagacgat atctcgaaat
     5521 cgtataactg caatcttgcc cgaattttac gaaaacgtct gcatcgtttg ggtatcagag
     5581 agggtttcag ggtagtctat tcaagcgaga aagccgacaa gtcttcggtc atcgaagagg
     5641 aaagcctcaa taagaaatcg aatgtgggaa caatttctta catgcccgcc atttttggtt
     5701 gttttatggc agcagagatc attcggtcgc ttacggaaaa accggaataa tagcaatgtt
     5761 ttttactttt ttataaacag gaaccggcca tcgtctttac acccgccata catacaaaac
     5821 aagggtatcg aaatggctcc ggctttcata atgccgttgc gtatttcctg ctgcaaatag
     5881 tagctatcgt ttatagggaa taaatttttc gataagaaat tgatgatgac agcagcaaaa
     5941 ggagaatgac tctgcgtacc atcggaggca atctgatcgt tggaagagcc aataaatatt
     6001 tttgtttcct ggttaagaaa gtttttatac acgatgcttt tttccgaaaa tttttcattg
     6061 aatatgatat tcgtttgtgg gatttcgatg gcattgggtt tcacacacaa ttcttcatcg
     6121 aacatggtac cactgtgaca gatatcgaga agtatcaggc tattcgtgca atcgagctgc
     6181 cccacaaaac gtttaagact cgacagtaga aatgtatttg aaaggaaatt cccgtcaaaa
     6241 tcgctgtctt tgaatatcag gcaaccttcg tcaagctcat tcttttcgcc atgcgacgaa
     6301 atatacagca agaactggct accttcatca aaagtatatt tgcttcttat ttccttaagt
     6361 ttgattttaa aatcactaca ggtcgggttc tcgagatata caatttgtgt aaaatagttt
     6421 gcgccaagca aatcttttat ttccctcgag tcagaaacag cattgttcag tttgttaaag
     6481 ttatcgaaat tgttgcaacc cataataaag cataaagttt ctccctttct tatatgcatc
     6541 tttgagtcgt tttcaagaga atcgttctgg taattaatcg tgatactctt tgtgacttca
     6601 tccacaatca gcgatttata cagctctttt tccgaaaaat attcaagttc cattcgcttt
     6661 tcaccaattg aatcggaaaa aaagtacagg ctctgcagaa aatgtgtgct gctacgatgc
     6721 gaacttgaga acacatccga cagcctgatt cctttatttt tcacatacga catggtgaac
     6781 aaacctccac cataggatgc tgcttcaaaa gccataggtg ttgataacac aattctgttc
     6841 aactgactga tggcactctc gtcgggccgg tttacaaaaa agcgttgcaa ctgacctata
     6901 aatttatccc catagctgat gtcgaggtaa tacaactgat taactgcata aatcgactgc
     6961 gaccagccga ataactcctc cgatgtaatt tcttctgaat ttccaagaac aatagatcca
     7021 tatttgccat aggctgctgc ataaaaaatt aaaatatctc ctgcctgcgc tttcgtactg
     7081 atttcgagaa aggcattcct gatgctttct tttgttgcct ccttgcctaa cagcaggtat
     7141 gaaccggcaa ttaccagcga gctgtcagtt tttgccctgt ttataaaagc agctgcatct
     7201 gactcacaat acaacaactt tgaaaattcc tgagaattat actcattaat accaacagaa
     7261 ataataaaag ttaatgctgg gcttttcatg gcaggtctgg cacccccata aaccggattg
     7321 caaagcatca ggaacaacga aatcgtgcaa caggtgaata aactaatttt cttgatcata
     7381 tctcatggtt gaatcattat tcaaagataa tgaattctat gcacacatca atgtgtcgaa
     7441 gcatagttgc ccataagagg taagagcaat ttttctattg aacagtgggt tccatttaag
     7501 tttggctctg aatcaatcta tcagcaatat ttcagccctt tcagcaattt cattttcaag
     7561 gtaaagaatt tctttccacg attggggtat ttcggaatag ccatagtatg caccggcaat
     7621 ctgaccggta actgcaccaa cggtgtcgga atcgttgccc aaatttactg ctttaagcaa
     7681 acaatccctg aaattatcgg ttccagcaaa tgcccatagt gccgattcaa gggttgcaat
     7741 gcaatatccg ctcgaattta tctgcgcttc gtttttatga atatacgagc cgttcagaat
     7801 ataagagata tttggcagca tatctggtat ttttttctga gcaccttgaa taaattcagc
     7861 cgagaaaatg aaattcttat ctttatcatg cagaaaagcc tgaagataca gccccagcaa
     7921 catacacgac tgcactgcct caggagcggc atgggtagtt ttcgaactca atgcggcata
     7981 ctccattagc aaatctgtat cgttgtggta tttaatagca accggagcaa ggcgcatgat
     8041 ggagccattt ccggcagtat tcgggttttc gcttccacaa tacacttctc cggtcttttc
     8101 aaaagtccgc agtgcatttc tggtggttaa accaatatcg aagcagcctc tctccgaact
     8161 gttatatcca ttccgatacc agtcgaggta tttttccatc tggtcatgtg catcaaatcc
     8221 acccttctct atcaggctat cagccagaca tagggccatc gaagtatcgt cagtccattt
     8281 gccagcctgc aatgaaaaag gacctccgcc aataatattg ctaataggct caaaacttcc
     8341 tctggcagta aactccagtg taacgcccag tgcatcgcct attgcaagac ccacaagcat
     8401 cccttttccc ttttcattca gcattgatga aaatttttgt aaaattacaa acatatttta
     8461 ggttattccg aagcatcaca tattttacaa catgctgtat ataacaatcg cctgattgtt
     8521 caattctgtg tatagaccaa aacatgagct taaaacaatc aatattatta ataataatta
     8581 tcttttttgt agcaaactat tctaatgcac aatatagtag aatgtatgat ttacaaaata
     8641 agcagattac acaagattca ttacatcttg acagtatagt aagaattaat tttagttaca
     8701 ttccttggga cattcatgat tacggagctt tcacagaaaa tgagattcga tattttcaag
     8761 gaaaaaccag ttattttagt atagtgaatc aagatagcat aaaatatttt gtcaattctt
     8821 tcaatattct aaatttaagt atttttgaaa aagagaacaa taaaaaaaag agaaaaccag
     8881 aagcacaaat tctttttgat ccaagaatgg tcattgattt tatatataaa tcaggaagaa
     8941 ttgaaacctt attgcttgat agaaatggaa atatatatta cgatgcaaat aagtacttta
     9001 caaacataaa gtttaatgta atagtcatag aaactgttcc accggcagaa cgtaaatggt
     9061 gggagccacc atttacaaaa ccagaagaag atgagtagag atgtgcttaa tctcgaagca
     9121 aaatgttatg atagtgatgc gagtatttgg ttgagttttg acccgatggc agaccaaaga
     9181 agctgggtta gtccgtatag ttattgtcag aatagcccgg ttatgagggt ggatcccaac
     9241 taatcttagt gccgaaaact ttcgggactg cgattaggag aaagcaatcg aagtattaaa
     9301 cgcatcagca attttttaaa acatcatgaa aaacttaatc agtaataatt ttgtaatttt
     9361 aacattttaa acaatttcaa ttgcattaaa agagaaatga aagaaataaa atcaaacata
     9421 attgggagtt tggtatttaa agacgtttgt caactaattg aacaaacaaa atcaggtata
     9481 gccagggtag ttaattcaga aataacaatg ctttattggt atatcggagg tcgtatcaat
     9541 gctgaaattc ttgaaaacaa gcgggcagaa tatgggaaac aaattgtgtc gcaacttgcg
     9601 acattattaa ctcagaacta tggaaaagag tttgaaatta ggaatttaag aagaatgatt
     9661 caattttcaa ttcttttctc gaataaagaa aaagtgtcgc aggctgcgac aaaattgact
     9721 tggtcgcatt ttatcgaatt gttaacaata aaagatgatt tgcaacgaga gttttatctc
     9781 ttaatggcaa ttcacgaagg ctgggggcgc gacacattgc gaaaacaaat tgcatcaatg
     9841 ctttttgaac gaactgcaat tagtggtaaa cccgaagatt ttattaaaaa gggattgtct
     9901 gatttgagtg aaaataataa gatttcgccg gaacttgttt ttaaaagtcc atatttttta
     9961 aactttacag gattaaaagg atacttttcg gaaaaaactc ttgaagacat tttaattggt
    10021 gagttagaac gttttatttt ggagttgggg aatgggtttt cgtttgtcga acgacagaaa
    10081 cgcatgatca ttgatggcga agacttttat ttagatttgc tcttttatca tcgaaaatta
    10141 aaaagactta ttgcgattga attaaaaatt acaaaattta aagcagctta taaaggtcaa
    10201 atggagcttt atcttgaatg gttaaacaag tatgaacgac aagatggtga agaaagtccg
    10261 cttggattaa ttctttgtac aaacggtgga accgaacaaa ttgagctgct taaacttgat
    10321 gaagccggta ttaaagtagc tcaatattat actgaattac ctgataaaaa gcttctggag
    10381 gagcaattac gaaaatcaat tgaagttgca aaaatgcgaa tgagtgaaaa tcaataaact
    10441 cgaatagaaa atgctacacg aaatttgcaa tatcagttgt tatgtttatg atgttttacg
    10501 tcatcgtgta aattgcgcaa cctcatttag aactaacatt gtgggccgcg cttaccagca
    10561 tttacagtat ttcccgttcg gtgagctgtt tgtaagccag cgaaactcat cgttcgattc
    10621 acgctacaaa ttctcagcta aagagttaga caacgaaaca ggatacagct atttcggcgc
    10681 aagatattat gatagcgatt tgagtagctg gctgagtgtt gacccgatga gtgataaata
    10741 tccgggcttg agtccctatg tgttctgctc taacaaccca gcaaaccgca tagaccgcga
    10801 tgggatgaaa gacacaacat ttgttgcagg taaagataag caagtatccc caaaaacagg
    10861 aacagcaaca cctattagga atccagacgg aacaataaac acaaatgctt acaattgtca
    10921 ttcttacgct tgggaaagtt cacagggcga tccaactgac cctcgcaata caaatcttgt
    10981 tcaaaatggt gttactaaat gggataataa tcccgataat aatatgagca gttatacaca
    11041 attaggtgca aatgatccta atcaaaaagg ggatagagtg atttattaca cagacgcaaa
    11101 tggaaatggc agatatgaca tgggcgagaa tatcgaacac tccgcgattg ttcaaacagt
    11161 tgataaaaat gggaatacag caactgtaat aggaaaaatg gggcaggctg gtatttctga
    11221 gaatcacccg actgccccag gttactatgt tattgacgcg aaaggaaatc ctacgagtag
    11281 agcatatttc agaagtccaa gctcttcgac tgtatcttca cctgagctgt ctttccccga
    11341 tctgaaattt gacccttatg taatgcaaag aaatgcaact tatgttgcgc ctcctataat
    11401 aattccaatt agaaaagagt aattatgaaa attagtaaat gtttattatt gctgtttatg
    11461 ggcttatttt cttgtaatgg gccaaatgct tgtgattgca gtttgtcgga aaaatcactt
    11521 tcttccataa cctacgaaga agcattattt ttgaacaaaa aaataattcc ctgtctaatt
    11581 gatagcatag atatattgga aatttcattt attgggtttc agaatccaat tacttcggat
    11641 attggtaatt atcatttaaa tcaaaagggt attagatttg cctatttggt agattatatt
    11701 ttatcaaaag acagcgtcga aacagtcaat aaaacttgga atgatgaaga agactggatg
    11761 cattggtcag agttgacaaa accgtacaga atttacaaca atggaattat tgttaaacaa
    11821 gacagtaata ataaaccgat attagagcca ttaacgccca aagatatggt caatattaaa
    11881 aaaatgtatt ttgattggtg gaaacaaaac aaatacaagc caatagaaac actaagagag
    11941 gaatttagaa aaggaaatac cattttgcag ttgccttatg tatggattta gttagaaaac
    12001 actgcaaatg gttattgtac cattcgcgct ataagttttc ggcaaaagag ttggacaatg
    12061 aaacggggta cagctatttt ggagcaaggt attatgatag cgatttgagt agttggttga
    12121 gtgttgaccc aatggcaagt gaaagaagct gggtaagtcc gtatagttat tgccagaata
    12181 atccaatgaa tagagtggat ccgacggggg cgctggatat aattgatgat tactattttg
    12241 atcaaaatgg aaatttggta gacagagtag agactgaaac acccgataga ttttttgtac
    12301 aagatggttt tgaaattgat ccaaatgatc tcaatatggt tccagaacca agttttaaag
    12361 aaataacaat gaatagtgac attggaatta tagcaagaac tacatatgca gaaatgagaa
    12421 gtggagacaa taatgcaaaa tccattgtgg cggaatcaat agtaaatagg catagcttac
    12481 cagatggaac ttatgaaaaa gcaaatggga catattcagg aatagtaaat aggttctacg
    12541 atgctacaaa aatcggagac cccaattatg atgtatttac aaacccacaa aactattata
    12601 ataaaagtac aaaggaaaac accgcatgga caaacagtgt tggagctgcc gtgaaagcat
    12661 attatggaaa tagtaatgtc ggtcagggag ttatttttta taattcgcag agctcaacaa
    12721 tatatgataa aaatccaggt atgcaaaaga taaatctttc aatttctgtt caaggcatta
    12781 aaggcctttg gaagttaaaa taatcctatt tatgaaaact ttaatatttt taaatattct
    12841 cgttattttt atgggttgct catggagtaa caaaggatat gagtttaata acaaagggtt
    12901 gttcatagag attgccgaca cgactaagcc tgtgaattta ccaaatggca gctattataa
    12961 attaatttac ataaatgaat ttcagtacaa aatagaatgg ggaaacaggc aaattaaaaa
    13021 cattagtaaa gatacttttg atattagtgg tagtgggcct ctttatttgc tagaatcaag
    13081 caatgatttt attattttac aacaaggttg tggaatgaac tgtaaatatt ctgttgttct
    13141 gcctttaagt tccaaagtag aagaacaaac atatattttt actcttgcac ttgacactaa
    13201 agacaatttg atagcttatt tacctgaaga ccaaaagaac tttattgttg ttaagaatta
    13261 ccttaccgga aaagtaatgg agatagaaga ggatgattta tgtccggcgg cttcttcagg
    13321 agattgtatt gatacttgtt attttgacaa gtcttgtctt attataaaat ggcaaggatc
    13381 aaaatggcaa agcacacaac cagataccaa agagcgaaaa ataaaaatta atatttaaga
    13441 gttaaatagt aaagtgaaat gcaacatcag aaaaaaagcg tgaataaaat caagctccgg
    13501 aaacggggct ttttttattt agcgaaagcc tgtttcgttt tgaagttttg aattatgtta
    13561 tcaatcagaa aaaacatttt gttttttgtc tcgtcatcga gtttttcaat atcatcaatc
    13621 cgttttaaaa cttccttgtc atattgtgaa agtacgcctt caccaatgat ataatctacc
    13681 gacacattaa aaacctttga aatttttata atcatctcaa tggatggagt attttcattt
    13741 cgttcatagt tgccaataat ggttcttgat gcttcaacct tttgggcaag gtctgtttga
    13801 gaaagattac gcaactttct tagctgaatt attttgtctc cgatgtacaa catatcacat
    13861 ttgacttttg acttcagaca tctgacatgg aaatgtaaac ttctgtgttt attggtagtt
    13921 gcaatgttgt tttcttcatg ctctgcagaa aagcgcttac acagactggt tgccttacat
    13981 ccggaattgg cgacgaagga caccatccgc atacaggata ctaccctgat cccggaagta
    14041 aggattgaca ccctggttca tttcagccgg ctgaaagata ccgtcaccat cgaaaaggaa
    14101 aagctcacag tgcggatcca tcaggttcgc gatacggtgt acatcgaggc agtccaaaaa
    14161 ccggacacca ttgttcacgt aaaagaaata ccggtggaga agatcatcca tgaaaacccc
    14221 tatgcaaaag agaaacagca aaatgccggc tgggtacaag cactcgcttt aatactaacc
    14281 tgtggaatag tttatctgtt gattaagctc gacaaaaagg agctgtaagt ttccaccttt
    14341 actttatgaa aaatgccctg aaaaggacat ttttttattt ataccggtga taaagatgtt
    14401 tagtcaaata aaacaaagtg ccgattagac taaaacagat ttacaatcgg cgttactcat
    14461 atccgaagcg atccaataaa tctttcactc gtttttcttt tgttaaccgg tccatatttg
    14521 ccggtggtct gtgcccgcgt ttcaaagcat aggcaatgtt acttccacac tggggcgaac
    14581 aggcatacga atcatagcgt ttgctctcat agactttacc acacatccag cagaccttta
    14641 ggtaatttgt tctcttgatt tgccgtgtcg taaacccatg caccagatct gtaaactgtc
    14701 ggttttccgg aatcactttt acacctatga tattttgccg gcacatttgg ttgggggtaa
    14761 tcatttgttt attcatggtc tttttgttgt aaatctacat cactcgttta aagcgctttc
    14821 cgttaagtgg tattagccgt attaattgtt gttatttacc gttgtttgct gttatcatca
    14881 ataaaaagcg aaactcagga aagtcctggg tgacacctga acgatacttg aacgacattt
    14941 ggataatact tagataatat ttgtatgata tttataagat aatttggtaa taatttaaga
    15001 gaagcttaat gaatattaca tagtaatagc aaggctttta ctgtatgagc acagcagtaa
    15061 aacgcactca aaaataatag caataataca tgaaaaatag tagcaacttt ccgcatcacc
    15121 cgccttacct gtattatggg tgttttggct atattggggc gaatatggtt gtgttaaata
    15181 agggatagtt ggggaaatgt gcattatgag attctttaaa ataattgact cagtttttta
    15241 gttagtctaa ttagtttcac agatgtgtgg tcgattaacg aaaatatttt tagttttatt
    15301 tgtaataaat aaataaataa atggaaatca tacctttgtc tataaggttt gtttatgaga
    15361 gatataataa ctgttaattc tctataatta tgaaaaaaac actaattttt gttgaaatcg
    15421 catttctgtt cgcaattttt attttttacg ccagtgcttg caataaattg ccgcagtgcg
    15481 aaaacccaca agcagcaacc atagtttatt ttggtgatga ttatgatacc tgtgcttgcg
    15541 atgaagaagt ttatattgtt cttgatgata tgccaaatga tacatttgtt atcggaaata
    15601 aaattcaaaa taaatacaaa aggaatgggc ttaatgtaga agtagaatgg gattgggcta
    15661 atcatcttat tggacctcaa atgggtgatg aaccttatcc ttcaaattgg tgtgggccaa
    15721 aatttccaat tagaattaat attttatgta taaataaatt ataggtttaa ttatgaaaag
    15781 aaaaattttg tttgctttac tcctgagcct tagtgtaaca gcattcactc aagtaaacta
    15841 tacatactat tctaataaca aacaggcgtg attgattaaa gttaaatgca aagctaagga
    15901 agcatgagat acttaagtaa cttactatta ttgattgcca cagtttgttc cggcaatgcc
    15961 ggtttttctc agcatggcac caaagactgg gaatgggagt gggcgccagt tggggctgta
    16021 tggttgtacg aaacagccaa catgggcacg aacaacattt atgcacaata ctggtatgtg
    16081 cggtcggcaa aagatactct ttttcacggg tttaactgcc gtaaattaga agtcgaacgc
    16141 cactactggc tcggtttcga gccagtaaag atgcccgata ggtttactta tcaggacggt
    16201 ggtgacatct atttttacaa tgccgacata aatgaatttg tactatcgtt cagctacgat
    16261 attcagcatg gcgatacggt aacttggcaa atgcctgttt ttgaacattg tgttaattcg
    16321 tattatttag atacaattct aaattgggac gtaggttatc tatccggtgc cggaaccgca
    16381 tatacacctt attctgtttt tatgtatagc aatgtgccta taagttcata tagaatacgg
    16441 cgtgttgaga ataattgttt tgcaggcgaa ttatcaacaa atactaatta tagtcatgga
    16501 cgttatttag atttcatcgg gactggttct gatttatatg agcttacaat tttagaagaa
    16561 atgcaaacaa gttgccaatg ttactatgat ggaagtgtaa taataaatct agcaatcgtt
    16621 tcaataaata atgacacaat aatttatgct gaagattcct gtctgaacta ttacaacaag
    16681 tttaacggca tttcatcgca tatcggaaaa aagtcgctta gagtttaccc taaccccttt
    16741 acagacctta ttaatgttga tcttcccgct atagaaggca actacgacat cagtgtctac
    16801 aatctcatgg gacaactcct ctattcagat aaaacacgta gcaacaacac tccattacat
    16861 ttaagccggc ttgttaacgg aacctatctc ctgcaaatac gaacaaacga taatgtcttt
    16921 aaccaacgta taatcaaaaa ctaaggaatt atgtaacgat tatttatttt gtcagtattg
    16981 tttctttatg ccgcaacatc aatggcccaa accgtaaaac gatacggtta tcatcgcatg
    17041 gaagaaaaaa aacttatcaa tttacagcat ccctattcca gttgagtcct ctctgaatga
    17101 ctcggctgga gaggagattc atacaaatcg aaaaatattt ttggataatt caatttgtta
    17161 ctttaaattt acattgtttt tattaacata tactttaatt ttacatgtat ttacgaaggc
    17221 aaatatttga agggattggc aaagaatcgg cttttttatg gggagcacga caaacaggaa
    17281 aaaccacact tctgaaaatg atgtttcccg attcaatgta tattgatttg ttgctaaact
    17341 ccgaattcct aagattcagc caaaactcaa atttgctacg agaaattgtt gaggccaacc
    17401 caactgaaaa gccaataata attgacgaga ttcaaaggat tcccgagttg cttaatgagg
    17461 tacattggtt aatagttaat aaagggactc agtttatttt gagcggctcc agtccacgta
    17521 aaattattcg ttccggcgtt aatttactag gtggcagggc attgcgttac gaattatatc
    17581 cactggttta tcccgaaatt cacaatttcg atcttaacaa aattttgaat cggggagctt
    17641 tgccccgtca ttatttatct gaaaatccga aacgtttatt gggtgcatat attggttcat
    17701 atttgcgcga tgagatcatt tcggaggctc ggttgagaaa cgtgtcagcg tttacaaaat
    17761 tcctcgaaag tgcagcattc tcgaatgctg aattggtaaa ttattcaaat attgcaactg
    17821 attgtggcgt tagctcggta acggttaaag aatatttcgg catactcgaa gatacattaa
    17881 ttggtcgatt cgttccggct tttacgctga gagccaaaag aagggttgtt gcttcaccca
    17941 aattttattt tttcgacatt gcggttgcca actatttatt aaaccaaaat catttagccg
    18001 aaaaaagcga ggcatacggt aaagctttgg agcaattcat ttatcttgaa ctctatgcac
    18061 atagcaggta ttccggttta cattacaaca ttagttactg gcggactgca actcaaattg
    18121 aagttgattt tattctgggt gatggcgaaa tagccatcga agtaaaatcg aatgaaaaca
    18181 tacaggcaaa acatgtaaaa ggcctcaggc aatttgcttc ggaatatgat gtaaaacgat
    18241 tgattgttgt cagcaaagat ccttattacc gtaaaattga caacatagaa attatgccgg
    18301 tcgaaaagtt tttacaagaa ttatggtcga acaatgtaat aaagggataa aaaaacctca
    18361 caccgaagcg tgagggtttt cctgagtttg tggagctggc ggggatcgaa cccgcgtcca
    18421 aacaggagac aaaagggctt tctacatgcg tatcctgtta ttgattgtcg ggaattgtcg
    18481 cgaaaacagg catccaaaac aactccgtat cttctgtttc tcgctgtggt aacgaagctt
    18541 accacaacca ccccggaatt tctgccacct ccttgtcgaa ccggaaccgg gtctgtccat
    18601 ccgggagatg tcccgctcca acaccttgtg tcggaattag gctcatttta ctgtcgtaaa
    18661 attaagctgc aagagcaaag ttatctgcgc catttgtttg gtcgcggatc agtttaacgg
    18721 gttaatccac ctagcccggc atgcttacac aatcacctca tctgctgtca ataccatgtc
    18781 agccccaaac aaggctgcaa atatacaaaa attgcaccgc ttttgcaaga aaatctttga
    18841 tgttgcacaa attatgcagg ttttcgacct aaaaattgta tttttaccac cctgaactta
    18901 aaaatttcgt atatggcaac aaaaatccgt atcggtatac tgaaagaaac caaaactcca
    18961 cccgacagaa gagtagtgtt gacacctgaa aatgttctac aagcaatgaa gcattttccg
    19021 gaagcagaat ttgtggttca aaaaagcaca ctgcgctgct ttaccgataa tgaatatctc
    19081 gaaaaaggca tagccatggc tgatgatatg tcggattgcg acgttctgat gggagttaag
    19141 gaagtgcata taccggaact gataccaaac aagcaatacc attttttctc gcacactcac
    19201 aagaaacaaa gctacaaccg cccactgctt caagcgttgg taaaaaacca tattaccatg
    19261 gtcgaccatg aatgcctgac atacgaaaac ggagcccggg tagtagcatt tggccgctgg
    19321 gccggaatag tgggtgcgta taacgcaatg ctgggatggg ggttgcgcac cggagactac
    19381 cagctgaagc gtgccgtaga ttgtttcgac cgtaacgaaa tgatcggtga actgaaacat
    19441 gtgaaactgg ctaaaaatct aaaaattctc attacgggca aaggtcgcgt tggcatggga
    19501 gccatggaaa cactttcggg actgaaatta aaaatggtta cggcaaaaga atttctaacc
    19561 caacagttta acgaacctgt aatttgtcag atcgatgctg atgagtatgt ggaacacaaa
    19621 gacggcggct ggaaagattt tccttacttt ttcaaacatc cggcagaatt tgtatcgaca
    19681 ttttctcctt tctcgaaagt aaccgatatc tggatcccct gccatttctg ggatcagaat
    19741 tcaccagctt ttttaaaacc tgccgattac cacgctccgg attttcgcat taagctgatt
    19801 gccgatgtaa gttgcgacct tattgacccg ataccttcca cgctgcgcgc ttcaaccata
    19861 aaggagcctt tctatggata caatccgaaa accgaaaaag aatgtgctgc cttcgataag
    19921 aataacgtca cagtgatggc tgttgataac ctgcccggcg aactgccccg cgatgcttca
    19981 ggtgagtttg gggcacgctt tatcgaggaa gtaattccgg ctattgtagg caaagacgaa
    20041 aagggcatta ttatgcgggg aaccatttgc agccgcggta aacttaccga aaggtatgcc
    20101 tatcttgagg attttttgaa cgggaagtag ttagggtgaa tacaggagtc agaatacagg
    20161 agtcagaata cagaagtcag aagtcagaag tcagaagtcc agaagtcaga agtccagaaa
    20221 cccgttatct tttattgctt gcattaccca aaaaattatc ccaccagctt cagttgccga
    20281 atggtttcga ccggattttt cgatgcaaac acagtgttac ctgccaccag tgcatttaca
    20341 ccggctttca caagttcggg agcattttcg agggtaacgc cgccatccac ttcaataatt
    20401 aaatcagggt tgtcgaattg ctttaactcc gataacaatt ccagcttgcg gtaggtcgac
    20461 tgaataaaac tttgcccgcc aaagccggga tttacagaca tcaccagcac cagatcgata
    20521 tcggcaatca cattttcgag catctgaacc ggtgtatgtg gatttaatga gacgcctgct
    20581 ttcattcctg catttttaat ctcctgaata gtgcggtgca ggtgtgtaca ggcttcaaca
    20641 tgaaccgtaa ggatatcggc acctgcatca cgaaaggcct gaatatattt ttcgggcgac
    20701 acaatcatca gatgcacatc gaggggtttg tgcgcctctt ttttaatagc cttaaccaca
    20761 ggaagcccaa acgaaaggtt cgggacaaac acaccatcca taacatcgag atgaaaccag
    20821 tcggctttgc tgtggttgat catatcaata tcggacgaga ggttcagaaa gtttgccgac
    20881 aacatcgaag gagcaacaat tacactcata aacataccaa ttttatgcaa taaaggtaat
    20941 aatgattatt cagcaaaaaa caaatagtga ataattctgg caatttttta caaattgtag
    21001 tatttcagct ttttatgctt tgtggattgt ttcaggcgcc ggattgaggc atctttaatc
    21061 tgccgtacgc gttcacgtgt cagacagaaa tattcggcaa tttcatcaag tgtttttgca
    21121 tgttctccac ccaatccata atacattttc aggatttctg actcccgctt tcccaataca
    21181 ctcaatgtgc tgttgatttc acgcattaac gattctttca gcagagaata atcgggtgga
    21241 ttatcggtat taatatcaat aagatctgcc aatgttccat catcaggatc agaccccaca
    21301 ggagcatcca tcgacacatg gcggacatta ttcttcaggt tgtcttttat ctcgtacgca
    21361 gtcatttcta tttcttcggc aatctcttca gcggtaggct ccctttggaa ctgctgttca
    21421 agctttgagc taattgtgtg aattttattg atggcaccaa tcttattcaa aggcaaccgt
    21481 accatacgtc cctgctcggc aagagaatgc agaattgatt gtcttatcca ccatacagca
    21541 taagaaataa acttaaaacc gcgtgtttcg tcgaaacgta aggcagcctt aataaggccc
    21601 agattgcctt cgtttacaag atcctgtagg ctcaatccct gattttgata ctgctttgcc
    21661 acggaaacaa caaaacggag gttggaggtt accagtttct ccaaagctgc tctgtcgccc
    21721 atttttattt tgcgcgcaag ttctatctct tcttccggtg taatcatcac ttttcgtccg
    21781 atttcctgaa gatatttctc gaacgcaggc gtatcgcggt tagtaacttg cttaactatc
    21841 tttaactgac gcatttcatt tcgattttac acacatttat aaaacgcttt tcttcataaa
    21901 aaggttgctt tagctaaaca acaaatcgct tgaaatgttt ttaacatttt attagggcta
    21961 ataattttca ttttttttaa tcgtattatt atttttatta tcattgcact gaaatgttgg
    22021 attctcctcg tagagattaa gatttaccca caaaagaaaa tttttatttt atcatttaaa
    22081 catattttta atgtacgaca ttaatcaact taacgaaaag ctgttacctg agcttaaact
    22141 tatagcacgt gaccttggac ttgtaagaac cgacatgctg aaaaaagaag aacttgttta
    22201 taaaattctt gaccagcagg ctataaatgc atcggctata cctgacgaaa aaaagcaacg
    22261 accacccaga caacgtcgca gagctgaaaa aattacgata aatacccct
//
LOCUS       DAOOQP010000040        22002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2890_length_22002_cov_4.741012, whole genome shotgun sequence.
ACCESSION   DAOOQP010000040 DAOOQP010000000
VERSION     DAOOQP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22002
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2890_length_22002_cov_4.741012"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLK75_05980"
     CDS             <1..890
                     /locus_tag="PLK75_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38832.1"
                     /translation="SGLNASGVAVMQHVLSDVNASASFSKGYEPIWFSLRKAIECDDF
                     NNDGNNDALDIDSVAMQNHYGYADSYIITGVAGSYIGENERAATIIEVAPASPYATVR
                     NTDYDDNIPGTNLYAANYAIARNNAQHYCTRYNGVKNNMGDGTMIGIEENWNIMLDYS
                     SMCALHTSGNIQFMQYIPSLHELRLSYHTLDGDQACENEAVIFDTDVLFQLNSNISAE
                     KPGQLAGVIYTTGNSVFIKLPHGVATDTLKIYNSSGIMVQSFQVVDGINYFARSELMP
                     GMYIMHLTNCRLTSKTILD"
     gene            952..1527
                     /locus_tag="PLK75_05985"
     CDS             952..1527
                     /locus_tag="PLK75_05985"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOY38833.1"
                     /translation="MNTLIIYANYKNTSFNNTIKEILSETLHKNGHEVVLRDLYDIRF
                     NPVLTKEDLRMIDQSIFPPDIMEEQRYISRAELIFFIYPIWWSGMPAILKGYIERVFL
                     NGFAFTFENGKPRPLLTGKKAILFNTTGSQIVFASEEEKKALNLITEKSIFNFCGLQV
                     IGHHYFHAVSTVAPEVRENYIKQVKKIAESI"
     gene            1557..2729
                     /locus_tag="PLK75_05990"
     CDS             1557..2729
                     /locus_tag="PLK75_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38834.1"
                     /translation="MKLKLLRNYVPELSSFLSGRKKPLAVSVNITSNCNQHCIYCEIG
                     RNIPSDKKTNPDFDELKMLINEMSDLGIKRLAINGGEPFLYPHILALVEYAGSKNVRC
                     NITSNGMNIHMLSQADIDLLKKFSCEINISVDSMCEEIHNVTRGHKNALANAIKSARL
                     LIKNGIVPIFLTAISKYNYNDLLRSLTEVRKLGVKTVLYQPIISYSNYPDRQTIDNKA
                     ELNVSREQVPLLLAELRKILKYEFTHGVKTNVYRLLPWIESYLDFVNGSSQKYFWQKI
                     LPVFRCRDIYAIVDIAYDGEVQPCALCTSGIYLSGLREKNLTELWLDAGKNLRAGNEK
                     GNFPSFCNGCCNHFSRNMLYSVFRYPLINYKMFFKVFYLSIRRLFSTLIKQFYPNK"
     gene            2726..3517
                     /locus_tag="PLK75_05995"
     CDS             2726..3517
                     /locus_tag="PLK75_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY38835.1"
                     /translation="MKVILVTGASRGIGFETARQLAIEGNTVILGCRKPVDFSHQEAL
                     ADLNVHIVTLDVTSETDVRNVCNFIGSRFGCLDVLINNAGIGETGNTRKPGIRKSVRK
                     IPVLKNMYNMFKPLVKKVVSYRPDFGLQSTSVEKSKEIMDTNFYGVWRMCSAAIPLLR
                     KSKNAQIINVSSGMGLLTNLSGYLPAYSLSKASLNALSIMLANELAKKNIAVNAVCPG
                     WVRTDLGGPDAPLSVPEGVDTIVWLALQNHPETGKFFKKRQILDW"
     gene            3517..4917
                     /locus_tag="PLK75_06000"
     CDS             3517..4917
                     /locus_tag="PLK75_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38836.1"
                     /translation="MKIGLVFPNKDRRYKTIHLGFGYLVSYARIFHDDLEFVYLDTRT
                     AHKKEIQRFFSTQFDLIGITCFSPVYYEAISIFDRIKKIMPNTPVCLGGPYTTTIEED
                     IFIGTPAEYAVVGEGEITFSMLLSHLKGNVEISEIDGLMYRDSRGNIVKNKAREKIKD
                     LDTIPLPAWDIFNMDRYPMHRVIGSRGCPFSCVWCSSSAIWDYTFRQRSVSSVVDEVD
                     SLIKTYGKKIFVFSDNSFNANIAWLEEFCDALIRRKTDILWSVSFRADIITETVARKM
                     KEAGCFNVSVGIESANNTILKNIRKGTTIEKFAAGIKILKDAGIEIMSQYVIGSPGDT
                     IDTICESIEFAKKSGADYSNFYTVLPYRKTPQWDYIIENGSLYTKDIHHFHSINPRIV
                     FDTKDFSYSDRLKAIHMAKKNGFYSNQDKKSFLFDFAKETGQKIQQWLPKKAGDRIYL
                     FLKSIYRMNFIKKNNL"
     gene            4919..6004
                     /locus_tag="PLK75_06005"
     CDS             4919..6004
                     /locus_tag="PLK75_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY38837.1"
                     /translation="MIAAAIIYGLFIALLFLYLMYPLWLLIYSHKHNQTESFDVTAYE
                     VSVVLFSYNGGDFLKTKILFLLKEISKFRNAELIVIDDFSQDGSIESICSIENENNLR
                     IIRKCEHKGIPDSMNTAVQYASYSSIVFCDQRQQLECGAIALLVNKLEHDDCCAASSC
                     ISHISKDNCFSWIRRYENIIKNLESCSGSLIGVYGPLYAFKKSAFSPVPAGIILDDLY
                     LSLKILSKGKIIFVKDCIIYDENVDRLFNYKRTKRYVNGFAQIIADRQLMTELSPKHK
                     LMLFWHKYLRLVIPVLFIAVLILTVIYAFHSYIAFLLFSALMLGVIYSLTPDLPQFFL
                     TNSFRINTYYFIAILELMVRRIGSVLR"
     gene            6191..6508
                     /locus_tag="PLK75_06010"
     CDS             6191..6508
                     /locus_tag="PLK75_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOY38838.1"
                     /translation="MKKVLKQIWSVIFRFIKNKYLFSLVFFIVWITVFDTYNLLERTK
                     RINDLKKLKQEREFFKQEIETYKRQTTELFSNKENLEKFAREQYMMKKDNEDIFIVID
                     DSN"
     gene            complement(6505..7092)
                     /locus_tag="PLK75_06015"
     CDS             complement(6505..7092)
                     /locus_tag="PLK75_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOY38839.1"
                     /translation="MDLNIELLKIKSNDVKPCKGRILIAEPLLPDYLFSRSVILLTNN
                     QQDAFEGIILNHQSSMKVGEVMTDFENFDADLFIGGPVDNDVLFYLHRYGDLIPGSRH
                     VVADISFGGDYNTVLHLMKKGVLKNENIRFFIGYSGWDSGQLFAEINDNSWLVADIDS
                     SFVFSDESTLWERSLGYAGSRYRIWKNFPEDPEWN"
     gene            complement(7328..8920)
                     /locus_tag="PLK75_06020"
     CDS             complement(7328..8920)
                     /locus_tag="PLK75_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38840.1"
                     /translation="MKKIWLIIVAVALLAPVLAYLIYLGLQNKQDPLWLELDKMVKSD
                     QYFYIREKPQPIDSLFEAIQQDPAFRKFDGYELISDPFPLTMEYRKFIKTVDFIHKNK
                     RVIILGVTGSGRTTMIDRVSKFFAVDPDHIMSLSCVPQLEVEYHKQYVGSFENGKFYK
                     GAMLDFLERANADSLHNYVMIIDDIDMIYPSTFFGAALWNELENPSYNNIIPGYGKEV
                     AFPDNFYMLSTTKIGGGSVIKLNDEHFRRLSPNGVIKVYPDSLEFMMYLRESLVDSTL
                     EFQHRQTKKLLYTFIKINHYIEDEWGSGVVLGQWSTIRKSIKPEQYDEFVEKFRQHVA
                     SMNLGVTVNEKEISKIVYSANNDGMMLNSNFFAKSFLFVYDSGIFSEASVAILALVLS
                     SITGWVLYVRRKKLIRRYVVKVYDISADYSNEKIAFNDAISKIQEVKQEIDAMALNKK
                     INYTESLFIYNLIHDRLAMIAKTEETESMYNRMFDSFMQDDVISESEYQKLHAFIEKS
                     RGKISENYYKQLLDKLERVKSK"
     gene            complement(9069..19016)
                     /locus_tag="PLK75_06025"
     CDS             complement(9069..19016)
                     /locus_tag="PLK75_06025"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOY38841.1"
                     /translation="MMVKSSFSRISAALLLLLFVMVTSVQAQTVTNGNFESWTAGTPD
                     GWTLNIVSSSLQFSQEVGGGVSGDAMKILALANPAGYDGTFANTVTGITPGKYYDLSL
                     FVKSNDDLMKLRYFSIKWLDESNVQIGSDINENTYNANTANAWTEFLLLTNPIQAPAG
                     AAKLVVGFRIYNGTGYSPNVTTMWVDNLTVTEHVFIDPGRTAGDVTQTPDVVGGTLNY
                     VFTPLDYTCAITQDAGNEVPWVSTNGSFATVSGNWIGVAIDFPDAFTGASDIVSYKID
                     GVVQTAPHLSAEEIAAGELWFYFEMQLLPDATHTLIINWDGNDNYQTETFVINANGLV
                     LQENPSIQEGIDNTEFSLTPPSQVITTTGTAVINQNMDIPLLGDDFPVDLYADVRFEF
                     NPALPADAVISIMSGAVEIANYTATGGETSLWLLEQMSSTLDRVPITSIDNNEYNYEI
                     SISNLSVETTYHVTSSLIVDYQAGFTVPTDWFVFGTDLADIIVSNTPVFTLPFYEGFE
                     NDFTYLDNAEDNTVDFTVVTNIVFAGNKAAFVDYSSSNTSILQMLGPLNLTAAVNPVL
                     KFAQIAETEGNYDHCYIEVSTDGGSTWTILPTSAYLGSGIYALPSYNTPEGPCFTEDS
                     YTAWDTPSVVDNTWWRKEKFSLATYAGQANVMVRFRLKPDTSVERQGWFIDAVEIYDE
                     LCTEPMAFTVQTIWSDGATLGWTSNESGWNIEVGPVAFNPGTGAEAFSFEGIDTNPYT
                     ITGLLPAHTYYAYIQSDCGTDGNSVWTGPVVFTTLVACPAPTGVTVSNITTSGATLTW
                     TQPGTPDTWDIIYGPSGFIPALEGTLISNTTEKPYAITGLTGNTTYQVYVRADCGTSN
                     SEWAPVISFTTLCDPTPITIVQGFNTTGTNVFPQCWSQQTVTGTSNITFVTSGTYPTV
                     AAPYEGSRMVYWNSDVITNGNKTRLVSLPVSTVGQPSVDVEFYWYFGTDGGATSYLTE
                     GVQLQYSTDGVTWINAGQFLRRYNATAGWSQRTITLPGITANQPQLFVGFLFTSNAGY
                     NCYLDKVTIKPGPACLPPDSFTLSALNPTSATVVWTESYTDPAVGYDIYFGTSATAPT
                     SETAPTYSVASDVLTYSFSSLTQNTAYYAWIRSNCGSGSYSTWSPVLSFTTPCDPISV
                     FPWTENFDGVTTPALPGCWNQIDGNNDGDFWKTFTSYGVGSSIAACIYTDYNAGANND
                     YLVLPLFNLNGNQRLKYYVRSYSTSEPNDYRVVLSTTGNAPENFTTVLKPLTTVNSAT
                     MTEISPIDLSAYSGNVYIAIHVPQGGLDGWYLCFDNFTVEDIPSCAEPTLLAASNIAG
                     SSATLSWTASLTDPANGYEIYYSTVNTAPNESTVANTSVAAGVTTCNLTGLNGLTTYY
                     AWVRAYCATDDYSFWTGPVTFTTLCAPVTTYPHIEPFTTYLPNTCWTEGDAGNLTAGP
                     STMSATASSWINDGFLNSGTTGAPRITLDATGDNDWIISPQFEIPDSPELRLKYNVGA
                     TQAGATTAPTTAWEADDYVQVLINAGAYNNWQELFIYNSSNVPSNLGQLAIHDLSAYA
                     GQTVRFAFRGVEGTTNGSASIDFFFDNFTVEITPTCETPSNLAVGTISQTTADISWTA
                     STSNPAGGYDIYYSTSSIAPDATTVPSASVAAGVTAYEITSLNASAIYYVWIRSVCGA
                     GDNSIWIGPVSFTTLCAPITTFPYLQSFGATIPACWSVSEGSTGASYHWAPTTADATH
                     GAAAPQSGSHFMYLYVYLSSSTYNPYYLTTEQFELGNEPKMLSYYYYLGASGYTSTPV
                     PLTVQISADDGATWTDLYVHNNSNSTFSTSSSGWNLNTISLSGYMNETVKFRFKSNSN
                     YGSGFCDQGIDEFSIYDLPSCIYPTALSSTGILSNSATVNWIASESTPSAGYDVYYST
                     STTPPEETTTPNENVAAGVTTINLTGLTASTTYYGWVRSDCGGGDYSSWAGPVSFTTA
                     CEPVNTYPYLQSFGAAMPNCWAASEGTAGASYHWAPTTADATHGAAAPQSGTHFMYLY
                     VYLASTTYNPYYLRTESFDLGSEPKLLSYYYYLGTGGYTTSPVPLTLQISTNGGTTWT
                     DLFAHTTSNSTFQSTSSGWVQNTVDLTEYAGAIATFRFMSNSNYGNSYCDQAIDEFSI
                     YVAPTCMPPTDLNVSGITSTEVDLNWTESTSGASGGYDIFYTSTTDIPDEATVPSVSV
                     AAGVTLQTLTGLTEMTDYNVWVRANCGGGDFSTWAGPLSFETQPTCPEPSGIAATNIT
                     TTSAQISWAENGDATVWDMVYGLTGFDPETEGITVSGLLSTDYLIDGTLNPSTIYQVY
                     VRADCGGGDYSEWVGPYSFTTLCGTIAAFPWTENFDAMPTIGSSVIPSCWKIETTTGT
                     PWASATASTNTYNDPFSAPNYMTCYYSPSSANKYLITPGFVLTAGVSYDFSFKFVGDN
                     YTGWTGDVRYNTSQTGTGSTVLGTAFLSSTTTSSTTYTEVSRNFVPATSGTYYFMVRV
                     YNTATPYYLGFDNFKFDATPTCVEVSSVAVSQVTNHTAQVTWQENGDATSWNILYGPT
                     GFDVQTEGTLVEGITSTSYLLTGLDPVTAYQVYVQADCGAGDLSYWSSPASFTTLVAC
                     PAVADLTLEDATYESLTLLIEPSGTETSWDVVYGLHGFVVGEGTYITVNENPFTITGL
                     SAETQYDFYIRANCGTDGTSTYSTVHIFSTTEMCPEVWDVAATDITFEGFTLGWDNSS
                     TEDTWNIEVGLPGFDPGTGNAVITLNGITANPYVIATGLEPETAYDVYVMADCDILGI
                     SDWYGPLAVTTNATCLAPVDVIISDVTNNTAEVTWTEAGTATLWNLVYGPTGFNPQTQ
                     GTLIEGLSSTSYIIENLDANTDYDVYVQADCGSDDVSDWSTVSPFSTECDIIDEFAWT
                     EEFTDWAAITSCWDLTGGTQTVTQYNGGAVEGSFWSWYNEEDAYLTSPLIDKSSLTNP
                     YMEFFWSHLYSTSYPDDRLDVEVSDDNGETWILVWTRFGPLFTSPGAGNTAPGTYITS
                     TNIDLSSFDDVIQFRFHFISGYGPDVFIDKVSIFDIICEEPTDLAVSDVDYTSAEVSW
                     TDVSGEGVADLIYGASGFDPLTEGTLVEEAENPYIIESLTPNTTYDVYVRSVCAVVGE
                     TEWIGPVTFTTLVHTGADILSYSFGSEIDHDPATIDANAKTVLVYVVEGTDLTNLVAE
                     FTLSDFANATVNGVQQVSATTPNDFSGGSLTYVVTAEDGNTVNNWVVTVEIWVGNSVN
                     NMSDIYVLPNPNNGKFVIHMDQKPEEFSYELIDVTGRVILSRTVEGKGVMDEMVDVKL
                     APGSYQLKFKSGKDVRIEKLIIE"
     gene            19195..19932
                     /locus_tag="PLK75_06030"
     CDS             19195..19932
                     /locus_tag="PLK75_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOY38842.1"
                     /translation="MRKKNYLSLSSIFLAAVLCFSGCEILTQLGNEMDKPNNSLTDSE
                     IISGLKEALKVGSQNAVNKLAAENGFYKDPVTFIKFPQEAIKVKEYALKLGMDQQVNK
                     FEETLNRAAEAAVKEAYPVFADAITKMTFSDAKNILQGNDDAATQYFKKQTWSQLHDL
                     FYPKVKQATEKVALTKYWDPLVTQYNKTTIVTGNPKIETDLNEYVTQKALEGLFIKLA
                     VEEKQIRENPAARVTDILKKVFGSLTN"
     gene            20875..>22002
                     /gene="ftcD"
                     /locus_tag="PLK75_06035"
     CDS             20875..>22002
                     /gene="ftcD"
                     /locus_tag="PLK75_06035"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOY38843.1"
                     /translation="MKQLIECVPNFSEGRDMTIIKQITDEIEKVSGVKLLDVDPGMAT
                     NRTVVTFVGTPDEVLEAAFSAVKKASEVIDMLKHTGAHPRFGATDVCPLIPVANISMD
                     EVVVYARKLGKRIGEELGIPVYCYENAAFTPERRNLAHCRSGEYEGLKDKIGKPEWKP
                     DFGEAKFNARSGATAVGARDFLIAVNFNLNTTSTRRANAIAFDVREKGRPKREGNPVT
                     GKIVTDANGEQVMIPGTLKGTKAIGWFIDEYGVAQVSMNITNISQTPLHIAFDEICSK
                     AAVRGVRVTGTEIVGLVPKKTLIEAGKYFLKKQQRSLGVSEEEILKIAVKSMGLDDLK
                     PFHPKEKIIEYLIESENTAKKLVDMTCTAFANETASESPAPG"
BASE COUNT         6668 a         4501 c         4562 g         6271 t
ORIGIN      
        1 tatcgggact gaatgcatcg ggtgtggctg taatgcagca tgttctttcg gatgttaatg
       61 catctgcttc tttttcgaag ggctacgagc ccatatggtt cagtctgagg aaagcgattg
      121 agtgcgatga ttttaataac gatggcaaca acgatgccct cgacatagat tctgtagcaa
      181 tgcaaaacca ttacggctat gccgacagtt atattattac aggggtagcg ggcagttata
      241 ttggcgaaaa cgagcgggct gccaccatca tagaagttgc tccggcttct ccctatgcta
      301 ccgtgcgcaa taccgattac gatgacaaca taccggggac aaacctttat gcggctaatt
      361 atgccattgc aaggaacaat gcacaacatt attgcaccag gtataatggt gtaaaaaaca
      421 atatgggaga tggcaccatg atcggcatag aagaaaactg gaatatcatg ctcgactaca
      481 gcagtatgtg tgcattgcat acaagcggga atatccagtt catgcagtac attccatcac
      541 tgcatgaact aaggctctca tatcacacac ttgatggaga tcaagcctgt gaaaacgaag
      601 cggtcatttt cgataccgat gttttgtttc aactcaatag caacatatct gctgaaaagc
      661 cagggcaact ggccggggta atatacacga cgggcaactc tgtgtttata aaactacctc
      721 atggtgtagc aaccgacact ctgaaaatat acaattcatc aggaatcatg gttcaatcat
      781 ttcaggttgt tgatggcatt aactatttcg ccagatcgga actgatgccc ggaatgtaca
      841 ttatgcacct gaccaattgc agattaactt caaaaaccat tctcgactga atgaacaatc
      901 atgggtttgc tttgtttcaa ttatatattg aatgtaagat agattaaaga tatgaatacc
      961 ctaatcattt acgcaaacta caaaaacacc agtttcaaca acaccataaa agagatactt
     1021 tcagaaacct tgcataaaaa tggtcatgag gttgtgctgc gcgatttgta tgatatacgg
     1081 ttcaatccgg tacttacgaa agaagacctg agaatgatcg atcaatcgat ttttccaccc
     1141 gacatcatgg aggagcagcg ttatatttcc agagctgagc tgatattttt tatttaccct
     1201 atttggtggt caggaatgcc agcaatactg aaaggataca tcgaaagggt gtttctcaat
     1261 ggatttgctt ttacttttga aaatggaaaa ccacgcccgt tgctaaccgg aaagaaagca
     1321 attcttttca atactaccgg ctcgcaaatc gtatttgcgt cggaagagga gaaaaaagca
     1381 ctgaatctga taacagaaaa gtcaattttc aatttctgcg gattacaagt aattggacat
     1441 cattattttc atgctgtttc gactgttgca cccgaagtac gtgaaaacta tattaaacag
     1501 gtaaaaaaaa ttgccgaaag tatttgatat gatagtttta tgtacttttg ggtattatga
     1561 agttaaaact gctacgaaat tatgttcctg aattaagttc ttttttaagt ggtcggaaaa
     1621 agccccttgc agtcagtgta aatattactt caaattgtaa tcagcattgt atttattgcg
     1681 aaataggaag aaatattcca tcggataaaa aaacaaaccc tgattttgat gagttaaaaa
     1741 tgctcattaa cgaaatgtct gatttaggaa taaaacgatt ggctatcaat ggtggtgagc
     1801 cctttcttta cccacatatt ttagctctgg ttgaatatgc aggttcaaaa aacgtacggt
     1861 gcaatattac ttccaatgga atgaatattc acatgctttc gcaagcagac atcgatcttt
     1921 taaaaaaatt tagttgcgaa attaatattt cggttgactc gatgtgcgag gaaattcata
     1981 atgtaacgcg tggacataaa aatgccctgg caaacgccat taaatcagct aggttgctta
     2041 ttaaaaacgg tattgttcca atatttctga ctgcaatatc aaagtacaat tacaacgatt
     2101 tgctcaggtc gctaactgaa gtcagaaaac tgggagtaaa aacagtattg tatcaaccaa
     2161 ttatttctta ttccaactac cccgacaggc aaaccatcga taataaagcc gaactaaatg
     2221 tttcacgtga gcaggttccc ttgctattgg cagagttgcg aaagatactg aaatatgagt
     2281 ttactcacgg tgttaaaacg aatgtttacc gtttgttgcc atggattgaa agctatcttg
     2341 actttgtaaa tggatcaagt caaaaatact tctggcagaa aatactaccg gtattcagat
     2401 gtcgtgatat ttatgctatc gttgatatag cttacgacgg agaagttcaa ccctgtgcac
     2461 tgtgtacatc aggaatttat ttatcgggac ttcgcgaaaa gaatcttacg gagctttggc
     2521 ttgatgcagg caaaaactta agagctggca acgaaaaggg caatttccct tcattctgca
     2581 atggatgttg taatcatttt tccagaaata tgctgtattc tgtttttcga tatccgctaa
     2641 tcaactataa aatgtttttc aaagtttttt atcttagtat aaggcggctt ttttcaactt
     2701 taatcaaaca attttaccca aataaatgaa agtaattctt gtaacaggtg catcgcgagg
     2761 cattggattt gagacagcca gacaacttgc aattgagggt aatacagtta ttttaggttg
     2821 tcgtaaaccc gtagattttt cacaccaaga ggcactagcc gatcttaatg tacacatagt
     2881 tacgcttgat gtcaccagcg aaaccgatgt gcgaaatgtt tgcaatttta ttggaagccg
     2941 gtttgggtgt cttgatgttt taataaacaa tgcaggtatt ggggaaacag gcaatacacg
     3001 taaacctggc atacgaaaat cggtcagaaa aattccagtc ctgaaaaata tgtacaatat
     3061 gtttaaacct ttggtcaaaa aagtggtatc atacaggcct gatttcggat tgcagagtac
     3121 ttctgtagaa aaatcaaaag aaataatgga tacaaacttc tatggagtat ggcgtatgtg
     3181 cagcgcagca attcctttac tccgtaaaag taaaaatgct cagataatca atgttagcag
     3241 cggaatgggg ttattgacga acctgtcggg atatctgcct gcttacagtc tttcgaaagc
     3301 ctcgctgaat gccctaagca taatgctcgc caatgaattg gctaaaaaga atattgcggt
     3361 aaatgcagtt tgtccgggtt gggtaagaac cgatttggga ggacccgatg ccccacttag
     3421 tgtacctgaa ggagtggata ctattgtatg gcttgcactg caaaatcacc ccgaaacggg
     3481 aaagtttttt aaaaaacggc aaatactcga ttggtaatga aaataggatt ggttttcccg
     3541 aacaaagaca gaaggtacaa aacaatacat cttggttttg gctacctggt ttcttatgcg
     3601 cgaatttttc acgatgacct tgaattcgtt tatctcgata cacgaacagc ccacaagaaa
     3661 gaaatacagc gtttcttttc aactcagttc gatttaatag gcattacctg tttctcgcca
     3721 gtatattacg aagccatcag cattttcgac agaataaaaa aaataatgcc caacactccg
     3781 gtttgtttgg gaggaccata taccaccact attgaagaag atatttttat cggaacacct
     3841 gcagaatatg ctgttgtcgg tgaaggagaa atcacctttt cgatgctgct gtcgcattta
     3901 aaaggcaacg tggagatttc ggaaatcgat ggtttaatgt atcgcgattc tcggggaaat
     3961 attgttaaaa acaaagcccg tgagaaaatt aaagacctcg acactattcc gcttccggcc
     4021 tgggacattt ttaatatgga ccggtacccg atgcacaggg ttattggctc ccgaggatgc
     4081 cctttttcgt gtgtttggtg cagttcttca gcaatttggg attatacttt ccggcagaga
     4141 agtgtttcca gtgttgttga tgaagtagat tctcttataa aaacctatgg taaaaagatt
     4201 tttgttttca gtgacaacag ttttaatgcc aatattgcct ggctcgaaga attttgcgac
     4261 gcattgatac gcagaaaaac cgatatttta tggtcggtct catttagagc cgatattatt
     4321 actgaaactg tagcacgaaa aatgaaagaa gcggggtgtt ttaatgtatc tgttggaatt
     4381 gaatcggcaa ataatacgat acttaaaaac ataagaaaag gaaccactat tgagaaattt
     4441 gctgcaggta ttaagattct aaaagatgca ggcatcgaaa tcatgtcgca gtatgttatt
     4501 ggttccccag gcgatacaat cgatacaatt tgcgaatcga ttgagtttgc caaaaaatca
     4561 ggcgccgact attcgaattt ttatacggtt ctaccctacc gtaaaacgcc acaatgggat
     4621 tacatcatcg agaatgggag tttatataca aaagacattc accattttca ttcgattaac
     4681 ccgaggattg tattcgacac gaaagacttt agttattccg atagattgaa agccattcat
     4741 atggcaaaga aaaacggttt ttacagcaat caggataaga aaagttttct gtttgatttt
     4801 gcaaaggaaa cagggcaaaa aattcaacag tggctaccta aaaaagccgg agaccgcata
     4861 tatctatttc tgaagtcgat ttaccgtatg aatttcataa agaaaaacaa tttgtagcat
     4921 gattgcagct gcaataatat acggtctctt tattgcatta cttttcttgt atctaatgta
     4981 tccattatgg cttctaattt attcccacaa gcacaaccaa actgaaagtt ttgatgtaac
     5041 tgcatacgaa gtaagtgttg tactcttttc atataatgga ggagattttc ttaaaacaaa
     5101 aatattgttt cttttaaaag aaatttcaaa attcagaaat gcagagctga tagtaattga
     5161 cgatttcagc caggatggga gtattgaaag tatttgtagc atagagaatg aaaacaatct
     5221 tcgaattatt cgcaaatgcg aacacaaagg tattcccgat tccatgaaca cggctgtgca
     5281 atatgcaagc tactcaagta ttgttttctg cgatcagcga cagcaactgg agtgcggagc
     5341 cattgccctg ttggtgaaca agcttgaaca tgacgactgc tgtgctgcat catcgtgtat
     5401 ttcgcatata tcgaaagata actgcttttc atggatccgc agatatgaaa atatcattaa
     5461 aaatcttgag tcgtgttctg gtagccttat tggtgtttat ggtcctttat acgcttttaa
     5521 aaagagtgct ttttcccctg ttcctgcagg catcattctt gatgaccttt atctcagttt
     5581 aaaaatcctg agcaaaggaa aaatcatctt tgtaaaagat tgcatcattt acgacgaaaa
     5641 cgtagataga ctttttaatt ataagagaac caaaagatat gtaaacggct ttgctcaaat
     5701 aattgcagac aggcagttaa tgacagaact ttcacctaag cataaattaa tgcttttttg
     5761 gcataaatat ttgaggcttg ttattcccgt gttgtttata gcagtactaa tactgaccgt
     5821 aatttacgct tttcatagct atattgcatt cctacttttt agtgctttga tgctgggtgt
     5881 gatatattca ttgacaccag atttacctca gttttttctc acaaattctt tcagaattaa
     5941 tacttattac tttattgcca tacttgagtt gatggtcaga agaattggaa gtgtattacg
     6001 gtagtgtgca ttgatgagaa tgaccccagt attgaaaata tcttttggtg ttacaaatac
     6061 tgaaaaaaac gacttcagaa agtttttaac attgctgata tctttcgtac ttactcacaa
     6121 tcagttgtga ataaatcgct tacctttaaa cataatttca gtttatattt gcattgttac
     6181 agattgaaaa atgaaaaaag tactaaagca aatctggtcg gtgattttta ggtttattaa
     6241 gaataaatac ctttttagcc ttgtattttt tattgtgtgg ataacggttt tcgacacata
     6301 caatttgctg gaaagaacaa aacgcatcaa tgacctgaaa aaacttaagc aggaaaggga
     6361 gtttttcaaa caggaaatcg aaacttacaa acggcagaca accgaattat tttcgaacaa
     6421 agaaaatctc gaaaaatttg cccgtgagca atatatgatg aaaaaagata atgaggatat
     6481 ttttattgtg atcgacgaca gcaattagtt ccattcagga tcttccggga aatttttcca
     6541 aattctatat ctgctgccag cgtaacccag tgagcgctcc caaagtgttg attcatcgct
     6601 gaaaacaaat gaactatcaa tgtcggcaac cagccaggag ttatcgttaa tttcagcaaa
     6661 caactgaccg ctgtcccatc ctgaataccc tataaaaaag cgtatgtttt catttttcaa
     6721 aactcccttt ttcattaaat gcaacaccgt attatagtcg ccaccaaacg aaatatcagc
     6781 aacaacatgc cttgatcctg gaataaggtc tccatagcgg tgcaaataaa agagaacatc
     6841 gttgtcaacg ggtccgccaa taaataaatc agcatcaaag ttttcgaaat cagtcataac
     6901 ctcacccact ttcatcgatg actggtgatt cagtataatt ccctcaaagg catcttgctg
     6961 gttattggtt agcaaaatca cacttcggct gaacaggtaa tcaggaagca aaggctctgc
     7021 aataaggatt cttcccttac agggcttcac gtcattcgac ttaattttaa gcaattcgat
     7081 attcagatcc atctcaactc ttcttaatac aaatatctgt aaaaaatcaa tagctcttta
     7141 caaataatta tatcttttta tagttatttc atcaatacat taaagttgtt aatataatta
     7201 acttcataag gtataacatt tatgtaatat atttgttatt attgcagaga aaaatatttt
     7261 ctctttttca tggttgattg gggttagtat aagaggcttg cggcaacccg ctgcggcctc
     7321 ttttctttta tttgcttttt actctctcca gcttatcgag taattgtttg tagtagtttt
     7381 ccgaaatttt acctcttgac ttttcaataa atgcgtgaag tttttgatat tcgctttccg
     7441 agattacatc atcctgcata aaagaatcga acatccggtt atacatcgat tctgtttctt
     7501 cagtttttgc aatcatagcc agcctgtcat gaatcagatt gtaaataaac aagctttcgg
     7561 tgtagttgat ttttttattc aatgccatgg catcgatttc ctgcttcact tcctgtattt
     7621 tgctaatagc atcattaaaa gcaatttttt cattggagta atctgccgat atatcgtaca
     7681 cttttacaac atagcgccgt attagttttt tccttcttac atacagtacc caaccagtaa
     7741 tcgacgatag taccaatgcc agaattgcaa cggaggcttc gctaaagatg ccggagtcgt
     7801 acacaaacaa gaacgatttt gcaaaaaagt ttgaattcaa catcatacca tcattgttgg
     7861 cactatacac tattttgctg atttcctttt cattaacggt aacacccaaa ttcatcgaag
     7921 ccacatgctg cctgaatttt tccacaaatt cgtcgtactg ctccggctta attgactttc
     7981 ggatagtgct ccattggcca agaaccacac cgctgcccca ctcgtcttcg atgtaatgat
     8041 tgattttaat aaatgtatac agaagcttct ttgtctgccg gtgttggaat tcaagggtcg
     8101 aatcaactaa cgattctctg agatacatca taaactcaag tgaatccgga tacactttta
     8161 ttactccatt gggacttaac ctcctgaaat gttcgtcatt aagtttaata acgctgccac
     8221 cgccaatttt tgttgtcgag agcatataga aattatcggg gaaagccact tctttgccgt
     8281 aaccggggat aatattgtta tagcttgggt tctcgagttc attccaaagt gcagcaccaa
     8341 aaaatgtaga aggataaatc atatcaatgt catcgataat catcacataa ttatgcagag
     8401 aatcggcatt tgcgcgctca aggaaatcga gcatggctcc tttgtagaat ttgccgtttt
     8461 cgaatgaacc aacatattgt ttgtgatatt ccacctcgag ctgtggtaca caactcagcg
     8521 acataatatg gtcaggatcc acggcaaaga acttcgaaac cctgtcgatc atggttgttc
     8581 ggccgctacc tgttacgcct aaaataatta cccttttgtt tttatgaata aagtctaccg
     8641 ttttaataaa tttacgatat tccattgtta aaggaaatgg atcggaaatg agttcatatc
     8701 cgtcaaattt cctaaaagca gggtcttgct gaattgcttc gaagaggcta tcaataggtt
     8761 gaggtttctc cctgatataa aaatactggt ctgatttcac cattttatcc agttctaacc
     8821 acaaggggtc ctgcttgttc tgaaggccca gatatatcag gtaagccaaa accggtgcaa
     8881 gcaatgcgac agcaactatt atcagccata tttttttcat ttcgctactt tccggttaca
     8941 ctgttcagta ttatgggtaa aaatacaaat aaaaatatac aaatttatat aattcaaaat
     9001 tacagtttct gagaaaaaaa agaggccggc tcgatatgag tcagcctcct tttttataaa
     9061 atcttgtttt actcgataat cagtttttcg atgcgaacat ctttccctga tttgaatttt
     9121 aactgatagg atcccggtgc aagcttcaca tcaaccatct catccataac acctttccct
     9181 tcaactgtcc tgctcaatat tacccttccg gttacatcaa tcaattcgta actaaattct
     9241 tcaggtttct gatccatgtg tatcacaaac ttaccgttat tcgggttggg tagcacataa
     9301 atatcactca tattgttaac tgagtttcct acccatattt caacagtaac aacccagttg
     9361 ttaacggtgt ttccatcttc tgccgtaacc acataagtta aagagcctcc tgaaaaatcg
     9421 tttggagtag ttgcactcac ttgctgaacg ccgttcacag tagcatttgc aaaatcgctg
     9481 agagtaaatt cggcaacaag attcgtcagg tcggttcctt cgacaacgta tactaataca
     9541 gtcttagcat ttgcatcaat tgttgcaggg tcgtgatcga tctcacttcc aaaagaataa
     9601 ctcaggatgt cagctcctgt atgaaccaac gtagtaaatg taactggacc aatccattct
     9661 gtttccccaa caacagcgca aaccgacctt acataaacgt cgtaggtagt gttgggtgtg
     9721 agactttcga ttatatacgg attttctgct tcctcaacaa gagttccttc agtaagagga
     9781 tcaaacccag aagcaccgta tatcagatcg gctacacctt cgcctgaaac atctgtccag
     9841 cttacttctg ctgatgtata atctacatcc gacacggcta aatcggtagg ttcttcgcaa
     9901 atgatatcaa aaatcgatac cttatcgata aacacatcag gtccgtagcc cgatataaaa
     9961 tggaagcgga attgaataac atcatcgaat gagcttaaat cgatattggt agatgttata
    10021 taagtaccag gagcagtgtt tccagcaccc ggagaagtaa agagcggacc aaaccttgtc
    10081 catacaagaa tccatgtttc accgttatca tcagaaacct ccacatccag tctgtcgtcg
    10141 ggataagatg tactatatag gtgcgaccag aaaaactcca tatatggatt ggtcagactc
    10201 gatttatcaa tcaaaggaga agtcaggtaa gcatcttcct cattatacca tgaccagaaa
    10261 ctgccctcaa cggctccgcc attgtactgt gttacagtct gtgtgccacc tgtaaggtcc
    10321 caacaacttg ttatggctgc ccaatcggtg aactcttctg tccaggcaaa ttcatcaatt
    10381 atatcgcatt ctgtagagaa cggcgacaca gtgctccaat cgcttacatc atcgctgccg
    10441 caatctgcct gaacatatac atcgtaatcg gtgtttgcat cgaggttttc aatgatatat
    10501 gaagtggacg acaatccttc aatcaatgtt ccctgagttt gaggattgaa tccggtggga
    10561 ccatatacca aattccataa tgttgctgtt cctgcttcgg tccatgttac ttcagcagtg
    10621 ttatttgtca catcactgat tattacgtcc acaggagcga gacaggttgc attagtagtc
    10681 acggctaaag gtccatacca gtcggatatt ccaagtatat cacaatcagc catcacatat
    10741 acatcataag cggtttcagg ttccagacca gttgcaataa catacgggtt agcggtaata
    10801 ccattcaggg taattacagc atttccggta ccagggtcaa atccgggcag gccaacttcg
    10861 atgttccatg tatcttcggt cgaagaattg tcccaaccca aagtgaatcc ttcgaaagta
    10921 atatccgtag cggcaacatc ccaaacctcc ggacacattt cggtggttga gaaaatatgc
    10981 accgtagaat aagtcgaagt tccatcagta ccacaattgg ctctgatata gaaatcgtac
    11041 tgggtttcag cagaaagtcc tgtaattgta aatggatttt catttacggt aatataggta
    11101 ccctctccaa caacaaatcc atgaagtccg taaactacat cccatgaagt ttcagttccc
    11161 gatggctcaa tcagtaatgt aagactttcg tatgtagcat cttcaagagt gagatctgca
    11221 acggcagggc aggcaaccaa agttgtaaaa cttgccgggc tgctccagta actcaaatct
    11281 ccggcaccac aatcagcctg aacatatacc tggtaggcag taaccggatc aagtccggta
    11341 agcaggtatg atgtagaggt aattccttca acaagagtac cttctgtttg aacatcgaaa
    11401 cctgtcgggc cataaagaat attccaggaa gtagcatcgc cattttcctg ccatgttacc
    11461 tgagctgtat gattcgtaac ctgcgaaaca gcaaccgaag aaacttcaac gcatgtaggg
    11521 gtagcatcaa atttaaaatt atcgaaacct aagtaatacg gagtagccgt gttatatacc
    11581 cttaccataa aataataggt tcctgaagtt gccggaacaa aatttcttga cacttcggta
    11641 taggttgttg aagaagttgt agtgctgctc agaaatgcag ttcccaatac agtggaacca
    11701 gtgcctgttt gagacgtatt gtaacgcaca tcaccggtcc atcctgtata attatcacca
    11761 acaaatttga agctaaagtc gtaagaaact ccggcagtaa gcacaaatcc aggagttatc
    11821 agatatttat ttgccgacga aggtgaataa tagcaagtca tgtaattagg tgctgagaac
    11881 ggatcattat aggtattggt cgaagcagtt gccgaagccc aaggggttcc tgtggtagtt
    11941 tcaattttcc agcatgatgg aattaccgat gaaccaatag taggcatagc atcgaaattt
    12001 tctgtccacg gaaaagcagc aatagtaccg caaagtgttg taaaactata tggtcctacc
    12061 cactcgctgt aatcacctcc gccacaatct gctctaacat acacctgata gattgttgat
    12121 ggattaagag ttccatcaat gaggtaatca gttgaaagca gtccggaaac cgttatgcct
    12181 tctgtttcgg ggtcaaaacc tgtaaggcca taaaccatat cccaaaccgt tgcatcaccg
    12241 ttttcagccc agctaatctg tgctgatgta gttgttatat tggttgcagc tatgccagag
    12301 ggttccggac aagtgggttg tgtttcgaag ctaagggggc ctgcccaggt actaaaatct
    12361 ccaccaccac aattagctcg tacccaaaca ttgtagtcag tcatttcagt aagcccagtc
    12421 aaagtttgca gcgtaactcc tgcagcaaca cttacactgg gtacggtagc ttcatcagga
    12481 atatcggtcg tagatgtgta gaaaatatca tacccacctg aagcacctga tgttgattct
    12541 gtccagttca aatcaacttc cgttgatgtt attccactga cattcaaatc ggtcggaggc
    12601 atacaggttg gagcaacata aatcgaaaat tcatcaattg cttggtcgca gtaactgttt
    12661 ccataattcg agtttgacat aaagcggaag gtagcaatag caccggcata ctccgtcaga
    12721 tcaactgtat tttgcaccca cccggaactt gttgactgga aggttgaatt cgatgttgtg
    12781 tgggcgaaca agtctgtcca tgtagttcca ccattcgtgc ttatttgcag cgtaagggga
    12841 acgggcgaag tagtataacc tccggtacca agataataat aataagagag cagtttgggt
    12901 tcagaaccaa ggtcgaaaga ttctgtcctc agatagtacg ggttataggt agtactggcg
    12961 aggtaaacat agagatacat aaaatgcgtc ccgctttggg gagcagcagc accgtgggta
    13021 gcatcggcag tggtcggtgc ccagtgatat gatgccccgg cagtaccctc agaagcagcc
    13081 cagcagttag gcatggctgc accgaagctt tgcagatacg gataggtatt tacaggttcg
    13141 caagcggtag taaagcttac tgggcctgcc catgagctat agtcaccacc accgcaatcc
    13201 gatcttaccc aaccgtaata ggtcgttgaa gctgtaagtc cagtaaggtt aattgtagta
    13261 acaccggcgg caacattttc attcggtgtt gttgtttctt cagggggtgt agttgacgtt
    13321 gaatagtaaa catcatatcc tgctgaaggg gtgctttctg aagctatcca gtttaccgtt
    13381 gcagagttcg acagaatgcc tgttgaactt aatgcagtgg ggtaaataca ggatggcagg
    13441 tcataaatac tgaattcatc tattccctga tcgcagaatc cactgccata atttgagttt
    13501 gatttgaacc ggaatttaac agtttcattc atataacctg acaggctaat ggtgttaagg
    13561 ttccagccac tggaagaggt cgaaaatgtt gagtttgagt tattgtgaac atacaaatct
    13621 gtccatgtag caccgtcgtc agcactgatc tgtaccgtaa gtggcactgg ggttgaagtg
    13681 tagcccgaag caccaagata gtaataatag gataacattt ttggctcgtt gccaagttca
    13741 aactgttcgg tggttaaata atacgggttg taggttgaac ttgacagata gacatataga
    13801 tacataaaat gagaccctga ctgaggtgca gcagcaccat gagttgcgtc ggctgtggtt
    13861 ggtgcccagt ggtagctggc gccagtgcta ccttcagaaa ccgaccagca agctgggatt
    13921 gttgccccaa agctttgtaa ataagggaaa gtcgttattg gtgcacataa agttgtaaag
    13981 cttaccggtc cgatccagat gctgttatca cctgcaccac acacggagcg aatccaaacg
    14041 taatagattg cagaagcgtt aagagatgta atctcataag ctgttacacc tgcggcaacc
    14101 gaagcacttg gaacggtggt agcatcggga gctattgagg aagtactgta atatatatca
    14161 tagccccctg caggattaga agtggaagca gtccatgaaa tatcggcagt agtctgactt
    14221 attgttccaa cagcaaggtt cgacggtgtt tcgcaggtag gggttatttc aaccgtaaaa
    14281 ttatcgaaaa agaagtcgat acttgcgcta ccgtttgtag tgccttcaac gcctctgaaa
    14341 gcgaatcgaa ccgtttgccc tgcataagcc gacaaatcgt gaattgcaag ctgccctaag
    14401 tttgacggaa catttgagct gttgtaaata aataattcct gccagttgtt ataagcccct
    14461 gcattaatta atacctgaac gtaatcgtcg gcttcccatg cagtggttgg agctgttgta
    14521 gcacctgcct gagtggcccc gacgttatat tttagtctca attcgggtga atccggtatt
    14581 tcgaactggg gagagataat ccagtcgtta tcgccggttg catcaagtgt tattctcggg
    14641 gcaccggtcg ttccgctatt cagaaaacca tcgttgatcc atgatgatgc agtagccgac
    14701 atggttgatg gtcctgcagt caaatttccc gcatcccctt ctgtccagca ggtattgggc
    14761 agatatgtgg taaatggctc aatatgagga tatgttgtta ctggagcaca tagagtcgta
    14821 aatgtaaccg gtcctgtcca gaagctgtaa tcatcggttg cacaataagc tctaacccat
    14881 gcataatagg ttgtaagacc attcaaacct gtaaggttac atgtggttac acctgctgca
    14941 acagaagtgt ttgccacagt cgattcgttg ggcgcagtgt taacagtgct gtagtaaatt
    15001 tcatatccgt ttgccgggtc agtaagcgat gcagtccacg ataatgttgc cgaagaaccg
    15061 gcaatatttg aagcagcgag caatgtaggt tctgcgcagg atgggatgtc ttcaacagta
    15121 aaattatcga aacaaagata ccatccgtcc aagcctccct gaggaacatg aatggcaata
    15181 taaacattcc cggaatatgc cgatagatca atgggagata tttctgtcat ggtagcagaa
    15241 tttaccgtgg tcagtggttt caacacagtg gtgaagttct caggcgcatt acctgttgtt
    15301 gaaagcacta ccctgtagtc gttcggttcg ctggttgaat aagaacggac gtagtatttc
    15361 agtcgctggt taccattgag gttgaacagc ggaagcacca gataatcatt gttggcacct
    15421 gcattataat cggtgtagat acaagctgca attgaactcc ctaccccata cgaagtaaag
    15481 gtcttccaaa agtcaccgtc attattccca tcaatctggt tccagcatcc aggcagcgca
    15541 ggagttgtaa ctccatcgaa attttccgtc catggaaata ctgaaatcgg gtcgcagggt
    15601 gttgtaaagg aaagtaccgg tgaccaggta ctgtagctac cggagccgca attcgaacga
    15661 atccaggcat aataggcagt attttgtgtt aatgatgaaa aactatacgt cagaacatca
    15721 gaagcaaccg aataagtagg agcagtttct gaggtcggag cagttgcact ggtgccgaaa
    15781 tatatgtcgt aaccgacggc aggatccgtg tatgattcag tccaaactac ggtagctgaa
    15841 gtgggattta aagcagaaag ggtaaaactg tcaggaggca ggcaggctgg gccgggctta
    15901 atggtcacct tgtcgagata gcagttatag ccggcatttg aggtgaataa gaagccaaca
    15961 aagagctgag gttggtttgc agtaataccg ggcagggtaa ttgttcgctg actccaccct
    16021 gcagttgcat tataccttct caggaactga ccggcattaa tccatgtaac accatcggtg
    16081 gagtattgta actgaacacc ttcggtgaga taagatgtag ctccgccatc tgtaccaaag
    16141 taccagtaga actcaacatc aacgctgggt tgcccaaccg tgcttaccgg caacgacaca
    16201 agtctggttt tattaccatt tgtaattaca tcgctgttcc agtaaaccat tcttgaacct
    16261 tcgtaaggag ccgccacagt aggatatgtc cccgaggtta caaaagtaat attcgaagta
    16321 cccgtaactg tttgttgcga ccagcattga ggaaatacat ttgtgcctgt agtattgaag
    16381 ccctgtacta tcgttattgg agttggatca catagtgttg taaagctgat aacgggagcc
    16441 cattcgctat tggaagttcc gcagtcagct ctaacgtaaa cctggtatgt tgtgtttccg
    16501 gtaaggcctg ttattgcata tggtttttct gttgtgttgc tgatcagtgt tccttccagt
    16561 gcaggaataa aacctgaagg accataaata atgtcccatg tatctggcgt gccgggttga
    16621 gtccatgtca aagttgcacc agaggttgta atattgctga ctgtaacccc tgtgggtgca
    16681 gggcaggcaa ccagagtagt aaatacaacc ggtcctgtcc aaacgctgtt accatcagtt
    16741 ccgcagtcgg actgaatata ggcataatat gtgtgtgccg gcaataaacc tgtaattgtg
    16801 tatgggttcg tgtcgatacc ttcaaatgag aatgcttcag caccggtacc agggttgaat
    16861 gcaaccgggc caacttcgat gttccaaccc gattcattgc tggtccaacc gagcgtagca
    16921 ccatccgacc atatcgtttg tactgtaaaa gccattggct ctgtgcaaag ttcatcgtat
    16981 atttcaactg catctataaa ccaaccctgt ctttctacag atgtatcagg tttcaaacgg
    17041 aatcgtacca tcacatttgc ctgacctgca taggttgcca gactgaattt ctccttacgc
    17101 caccaagtgt tatcaacaac cgatggtgta tcccatgcag tataggaatc ttcggtgaag
    17161 caaggtcctt cgggagtgtt atacgacgga agtgcatata tacctgaacc caaataagca
    17221 ctggttggta aaatcgtcca ggtagaacca ccatcggtcg acacttcgat gtaacaatgg
    17281 tcgtagttac cttctgtttc tgcaatttgt gcgaacttta aaaccggatt cactgcagcc
    17341 gtcagattca gaggtcccag catctgtaga atacttgtgt tcgaagatga ataatcaacg
    17401 aaagcagctt tatttcctgc aaaaacaata tttgttacca cagtaaaatc tactgtatta
    17461 tcttcagcat tgtcgagata ggtaaaatca ttttcaaaac cttcgtaaaa aggaagcgtg
    17521 aaaacagggg tgttcgatac aataatatcg gctaaatcag taccaaacac aaaccagtcg
    17581 gtgggtacag taaatccggc ctgataatca acaatcaagc ttgaggttac atggtaggtt
    17641 gtttcaaccg ataagttact gatgcttatt tcgtaattgt attcattatt atcgattgaa
    17701 gtaatcggaa ccctgtcgag ggttgacgac atctgctcaa gcagccagag ggaagtttcc
    17761 ccaccggtgg ctgtataatt tgcaatttca acagcaccgc tcattataga aattactgca
    17821 tcggcaggca atgcaggatt aaattcaaaa cgcacatcag catataagtc cacaggaaag
    17881 tcgtctccca gcagtggaat atccatattt tgatttataa ccgcagtgcc tgtagtggta
    17941 attacctggc tcggaggagt aaggctaaat tcggtattat cgattccttc ctgaatggat
    18001 gggttttcct gaagcacaag accatttgca ttaataacaa atgtttcggt ctgataatta
    18061 tcgtttccgt cccaattaat aataagtgta tgcgtagcat cgggcaacag ctgcatttcg
    18121 aaataaaacc atagttcacc tgctgcaatt tcctctgccg acaaatgtgg tgctgtttga
    18181 acaacgccat caattttgta gctcacaata tcggatgcac cggtaaaagc atccggaaag
    18241 tcaatagcaa caccaatcca gtttcctgaa actgttgcaa atgatccgtt agtcgatacc
    18301 caaggaacct catttcctgc atcctgagta atagcacagg tgtaatctag cggtgtgaat
    18361 acatagttga gtgtgccacc tacaacatcc ggagtttggg ttacatcacc tgcagtgcga
    18421 cccgggtcaa taaatacatg ttctgtaacc gtaagattat caacccacat cgtggtaaca
    18481 ttgggtgagt accctgtacc gttgtaaatt ctgaaaccta ccaccaattt ggcagcaccg
    18541 gcaggagcct gaatcggatt tgtcaacaga agaaattccg tccatgcatt tgcagtattg
    18601 gcattatagg tattttcgtt aatatcagag ccaatctgca cgttcgattc atcaagccat
    18661 tttatactaa aatagcgcaa cttcatcagg tcgtcattac ttttaacaaa aagcgacaaa
    18721 tcgtaatatt ttcccggggt aattccggta acggtattcg caaatgtacc atcataacct
    18781 gcagggttgg caagtgcaag tattttcatg gcatcacccg aaacgccccc tcctacttcc
    18841 tgtgaaaact gtaacgaact gcttacaata ttcaaggtcc atccatcagg agtacctgct
    18901 gtccatgact caaaattacc gttagtaacg gtttgggctt gtacacttgt taccatcaca
    18961 aaaagcagta acagcaatgc cgcagagatt ctcgaaaatg aagattttac catcatagtt
    19021 atttttgttt tattaattat ttacacacta tacacacaca caatcacaaa aatacaactt
    19081 aattaaactt aaacaactga ctgatataaa ctttgttcaa aaattatatc tatgttttta
    19141 tttgaaatgt aaaacaaaat aaagtacctt tgtttagtca ataaaaagtg gatcatgaga
    19201 aagaaaaact atttaagcct gagcagcatt tttcttgccg cagtattgtg tttttcgggt
    19261 tgtgaaattc tcactcaatt aggaaacgag atggataaac ccaacaactc gcttaccgat
    19321 tccgaaataa taagcggcct gaaagaagcc ctaaaagtag gttctcagaa cgcggtaaac
    19381 aaactggctg ctgaaaatgg attttacaaa gatccggtaa cgtttatcaa attcccgcag
    19441 gaagctatta aagttaagga atatgcattg aaactcggga tggatcagca ggtaaataaa
    19501 tttgaggaaa ccttgaaccg tgcagcagaa gcagccgtga aagaagccta tccggtgttt
    19561 gccgatgcta ttacaaaaat gactttttcc gatgcaaaaa atattcttca gggcaatgat
    19621 gatgctgcaa cacagtattt taaaaaacaa acatggagcc aacttcacga tcttttctat
    19681 ccgaaagtaa agcaggccac tgaaaaagtt gcccttacaa aatactggga tcccctggta
    19741 acgcagtaca ataaaacaac cattgtaacg gggaacccta aaatagaaac cgatttgaac
    19801 gaatatgtta cccagaaagc actcgaggga ttatttataa aactggctgt tgaagaaaaa
    19861 caaattcgtg aaaatcctgc agccagagta accgatattc ttaaaaaagt tttcggtagc
    19921 cttacgaatt gatcattttc agagactcag taaataaatg aattttcgaa aatgctgcgg
    19981 gtgatcgtgg catttttgtg tttttcggca accctgattt taattcatta ttaatttttt
    20041 tcctagcttg tccatttggg ttatgtcttt ttaagataaa cactgtggct agagcaaaag
    20101 attgtgatgt aaaagatcat taaatcattc tttctcttcc tttttcctta atgaaaaagt
    20161 aacaaaaaat caaaacatgg ttcggctgcg ctcaccagcc caatatgcta tctccccgct
    20221 ctacccgttt aatgaaaaac taacaaagta gtgttttgtc ggccttacac tacatgcagc
    20281 aagcgtccag gcttgctgca caaaacaaac ggtactggtt ataccaatga gccgacaaaa
    20341 caaagctgcg agaagcaaac cttgttcggg tctgcagatt gtttgcttct cttgcccttt
    20401 gttgtttttc tattaaacga gtattcacgc ccggattggc tcgcattttt gtcagggctg
    20461 cccacgttgt gatgcctaca gtggactgta aacttaagtg acttgtgaga atggaaatgc
    20521 aattgagaaa actgaacttt ataaatgagg ttgcgctgcg ggggatgata ctcatactat
    20581 attattacaa aacgatatgg atacgctaag ttcggtaaaa taaacattta gtagcctgca
    20641 atttagtgac aatatgctat ttacgatttg ttttctattg cggtggacaa ggctggagag
    20701 ctccgtcatt cttaaaaaag tttttggtag ccttacgaat taatcatttt cagagactca
    20761 gcaaataaat gaattttcga aaatgctgcg ggtgatcgtg gcatttttgt gtttttcggc
    20821 aatcctgatt ttaattcatt attaattatt ttctttgcaa aaaacattgc aattatgaaa
    20881 caacttatag aatgcgttcc caatttcagt gaagggcgcg acatgaccat tatcaagcag
    20941 attaccgatg agattgaaaa agtgagcggg gtaaaacttc tggatgtgga tccgggcatg
    21001 gcaacaaacc gcacggtagt taccttcgtg gggacacctg atgaagtgct tgaagctgct
    21061 ttttccgctg taaaaaaagc ttccgaagta attgatatgc taaaacatac gggagctcat
    21121 ccccgttttg gagctaccga cgtttgccct ttaattcccg ttgccaatat tagcatggac
    21181 gaggtagtgg tttacgctcg caaacttggc aaacgtattg gcgaagaact tggcatacct
    21241 gtgtattgtt acgaaaacgc agcctttaca cccgaaagac gtaatctggc gcattgcagg
    21301 tcgggagaat acgaaggact gaaagataaa atcggcaaac ccgaatggaa gcccgatttc
    21361 ggtgaagcaa aattcaacgc ccgctcagga gcaaccgcag tgggtgcccg cgattttctt
    21421 attgcagtga attttaacct gaacaccact tctacccgtc gggctaatgc cattgctttt
    21481 gacgtccgtg aaaaaggcag acccaaaagg gaaggcaatc cggttaccgg aaaaatcgta
    21541 accgacgcaa acggcgaaca ggtgatgata cccggcacac tcaaaggaac caaagccatc
    21601 ggctggttca tcgatgaata cggggttgct caggtatcga tgaacattac aaacatttct
    21661 caaacaccgt tgcacattgc gtttgacgaa atctgcagca aagcagccgt acggggtgtg
    21721 cgtgtcacag gaactgaaat tgttgggttg gtacccaaaa agaccctcat cgaagcaggc
    21781 aagtatttcc ttaaaaagca gcagcgttct ttgggcgtat cggaagaaga aatcctgaaa
    21841 atagccgtaa aatcgatggg tctcgacgat ttgaagccat tccatcccaa ggaaaaaata
    21901 attgagtacc tgatagaatc ggagaacaca gccaaaaagc tggttgatat gacctgcaca
    21961 gcttttgcca acgaaacagc atcggaatca cctgccccgg ga
//
LOCUS       DAOOQP010000041        21881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2918_length_21881_cov_5.601347, whole genome shotgun sequence.
ACCESSION   DAOOQP010000041 DAOOQP010000000
VERSION     DAOOQP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_21881_cov_5.601347"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLK75_06040"
     CDS             <1..639
                     /locus_tag="PLK75_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38844.1"
                     /translation="DLIRSFSDVPAEVLAVSNGLLHRNFITIGLLLKKLLLKNETKIK
                     TNNGLIPDNWIYVQEPEVSMGRIQVFNNWSPFMIADNSKVWLGLEYFCNPDDSLWTMA
                     DKDLIEMATAELAKIDIINPADVLDATVLRMENTYPVYYGSYYEFDTVRRFTDNIPNL
                     FLIGRSGMHRYNNSDHSMMTAITAVRNIVEGRTDKSNIWDVNTESDYHEQKK"
     gene            741..1166
                     /locus_tag="PLK75_06045"
     CDS             741..1166
                     /locus_tag="PLK75_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38845.1"
                     /translation="MEQSSIETAQRQLRTLAILHYIWGGLNIAGGIALIIIYTSVGIG
                     MVAAGAIHGEPDQIALGIFAGVFIMFAFTLGFFIIASAVLSIVSASFMLKRKNRVFSI
                     VIAGTECANAPLGVLLGVFTIVILSKPELRELYNANNSQ"
     gene            1177..2499
                     /locus_tag="PLK75_06050"
     CDS             1177..2499
                     /locus_tag="PLK75_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOY38846.1"
                     /translation="MKSAFSKLFVGLAILIIVVIPSCNLFEESIQPPEIQTIEVTEVT
                     YNSAISGGNITNDGGADVTARGMCWAVHTSPTVSDSLSNNGSGTGIFTATLTDLLPQT
                     IYYVRAWATNSEGTAYGEEFTFTTLQQPLVPSVTTAGVTDIGETTAVCGGNVTSDGSS
                     SLVAVGVCWSTAEQPTVDDNKTIDGTSTGEFLSSITALARNKTYYVRAYATNSLGTGY
                     GEEYSFTTLPSPTAGTVTDIQGNTYTTDVFGTQVWMTQNLRTIRYNDNTDVPIITDDA
                     QWSNLSTGGFSVFNNDIDNYYGLYGALYNWYAVQTGKLCPSGWHVPTDDEWKTLEMNL
                     GMSQTEADGSGTRGTTEGGEMKESGLENWAYPNTGATNESNFTGLPAGIRRVNGVYAE
                     NRTAGYFWSATDGGELNAWARLLSNSNSNVSRGQYYKQFGMSVRCVQD"
     gene            complement(2518..3300)
                     /locus_tag="PLK75_06055"
     CDS             complement(2518..3300)
                     /locus_tag="PLK75_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOY38847.1"
                     /translation="MCGRFILVQKLETIEKRFNLSPSLFDLSPSYNTGPGMYTPVVAS
                     NSKRCLQLFRFGMCPSWAKKDMFLLNARTEGDRNSENDVHYHGPKDIILKPSFRKPIR
                     SQRCLVIADAFIEGSTDEGLDKAHVVFLKNKVRPFAMAGIWDEWKNPVNGESVFSFAI
                     ITTVANELMQKIPHHRSPVILEPWQESVWLNNDAPLSDITAMLKPYDSNKMNAYPIGS
                     SVKNKRNNFPELLQPIGNNLNDEAEIVIIESLYRQGFGRKDA"
     gene            3554..5341
                     /locus_tag="PLK75_06060"
     CDS             3554..5341
                     /locus_tag="PLK75_06060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOY38848.1"
                     /translation="MDNFIVSARKYRPDNFDSVVGQDSITRTLKNSVKTRQLAQAYLF
                     CGPRGVGKTTCARIFAKTINCSNPTPEHEACNVCESCKTFNESRSLNIHELDAASNNS
                     VDDIRNLIDQVRIPPQIGKFSVYIIDEVHMLTSSAFNAFLKTLEEPPKHVVFILATTE
                     KHKIIPTILSRCQIFDFNRISIHDITARLKYVSQNEGITAETDALSIIAQKADGAMRD
                     ALSIFDQIVSFSGNNITYQNTIENLNVLDYEYYFRVTRVLLEHDYRGALNILNEVIEN
                     GFDPGVFLSGLASHLRDILMCKDPGTYKLLEVGENIQKKYHEFSGICDVDFLYKAMDI
                     ANESDFKYKVTRNKRLLVEICLMKISFINHQSNIPVKESRPVVTQEPSKSYPATGTAP
                     ERPIENKSNDEKQEQGPAVQPNESHNEGTVIKTDVSPKEEPGFRNGRIPSVSIKNIGK
                     NLTAQQSEPQTYEANDSTRVIVIGDDEFNTAKLLDKWQEFTAKLSDKPRLFQLFNNAN
                     PVLNPDWVIGLEIDNQNLYDLVMSIKPELLLYLQTALNNKNISLDLQIAEAPDNSGEL
                     LYTDEEKIQYMVSKKPELATLIKQLDLDL"
     gene            complement(5355..5753)
                     /locus_tag="PLK75_06065"
     CDS             complement(5355..5753)
                     /locus_tag="PLK75_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOY38849.1"
                     /translation="MSKVIAISEAAGIALHSMVLIARSENSINATEIANISESSRHHV
                     AKILQRLVKSGLISSVRGPSGGFRLKKSADNITLLEIYEAIEGKIALTSCPFDHANCV
                     MGKCILQQIANKVSDDFVRYLSETHLNTVL"
     gene            complement(5760..6002)
                     /locus_tag="PLK75_06070"
     CDS             complement(5760..6002)
                     /locus_tag="PLK75_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38850.1"
                     /translation="MQNEYEIAGIFIKDPSVAFQEVQKIMTSFGCVIRTRLGINNDEI
                     SGGIIIVDLHGDEKQKKLFLERIREVKNVELQEMKF"
     gene            complement(6007..6264)
                     /locus_tag="PLK75_06075"
     CDS             complement(6007..6264)
                     /locus_tag="PLK75_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38851.1"
                     /translation="MSDITIIGIHVLDRIKEAGKTQRVLSQHGNVLRTRLGFHEVSDY
                     KCSRNALIIIELKGNKADREALVSDLANIGGIEVKKMVFKP"
     gene            complement(6274..7704)
                     /gene="hydG"
                     /locus_tag="PLK75_06080"
     CDS             complement(6274..7704)
                     /gene="hydG"
                     /locus_tag="PLK75_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOY38852.1"
                     /translation="MIFKPQQYNIPDKRMQPFIDPEEIERILKENQNPSALRVREVIE
                     KSLNKERLSLEETAVLINADSAELIQEIKNGALRLKEKVYGNRIVLFAPLYIGNKCTN
                     NCQYCGFKASNKEAIRKTLTDEELVREIEALEDNGQKRLILVYGEHPDYSPEYIAHTV
                     RIAYGVKKKNGEIRRVNINAAPLEIDGFRIVHEAGIGTYQIFQETYHPEAYKKYHLGG
                     KKRDYNYRLTGLDRAQEAGVDDVGIGALFGLYDWRFEVMALVRHTNHLEACYNVGPHT
                     ISFPRIKSALGLNLDPMYEVNDDAFVRIIAILRLAVPYTGLILTARESARVRDEVISF
                     GVSQIDGGTKLELGSYSANLNEKQDLNKEQFAINDTRSLGEIIDELLSKNFIPSFCTA
                     CYRKGRTGEHFMEFSVPGFIKRYCTPNAILTLSEYLCDYATPEVAEKGWKLIETELVK
                     LGDYKNLPELKNRIEQIKNGKRDLYF"
     gene            complement(7778..8272)
                     /locus_tag="PLK75_06085"
     CDS             complement(7778..8272)
                     /locus_tag="PLK75_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOY38853.1"
                     /translation="MLKPSIEKILNEQIVKEGYSSFLYLSMAVWAENNKLSGTAKWLY
                     AQAEEEKEHMLKFVHYINERGGKAIIPAIEKAPEKWTDIHKLFEEVLKHEIYITESIH
                     KIVALTVKENDFATNNWIQWFVTEQLEEEANVNAILDRLSLLGKNAGAIYMFDRDVYR
                     MREE"
     gene            8385..8846
                     /locus_tag="PLK75_06090"
     CDS             8385..8846
                     /locus_tag="PLK75_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOY38854.1"
                     /translation="MDKIKIIRFDEVESILNSVQICTLAMIDNGKPYAVSMNFGYIDR
                     TIYFHGAPSGRKIDVLNMNKTVSAVFYTDAVLNVRHETVACSYSMKFRSVVVNGEIEF
                     VTNNDEKIIALNSVMQKFSDRTNFTYGLPALNNVNVFKLPVIDYTAYKRGY"
     gene            complement(8850..9554)
                     /locus_tag="PLK75_06095"
     CDS             complement(8850..9554)
                     /locus_tag="PLK75_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOY38855.1"
                     /translation="METIQKKYKYLFFDLDRTLYDFETNSTETLREIFEKYPLGKHFE
                     SFDEFHAIYRKHNDFLWNQYRSHIITKQDISWQRFQMTLEEKGIFNELMAKNMGTDYI
                     QHSPDKSRLFPCTHEVLAYLQHKYKLFLITNGFKEVQYRKIENCQLRPYFEQVFTSEE
                     IGFNKPEKEYFEIVLQKTGASVADSVVIGDDLEVDIKGANTIGLDTVWFNTVNKTANR
                     PVTYEINHLCKLKDIF"
     gene            9825..10700
                     /locus_tag="PLK75_06100"
     CDS             9825..10700
                     /locus_tag="PLK75_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOY38856.1"
                     /translation="MKKIADSELILNTDGTIFHLHLLPHQIADDILLVGDQGRVDMIS
                     KHFEKLETRVQNREFVTQTGVYNGKRITVISTGIGTDNIDIVVNELDALVNIDMQSRT
                     EKPEKKSLNFIRIGTSGGMQPYLNAGDFLVSDTAIGFDGLLNFYHDSQRVCRSDYEES
                     LISYLNYHTALPRPYVVDADKTMIDMLGEGLHHGVTISAPGFYGPQGRELRLGLVDPD
                     INSKISAYRFGKEKITNYEMESSAIFGLSKMLGHKAVTVCVIIASRFNKTALPDYKPK
                     VEELVQLVLDRITGR"
     gene            complement(10712..11587)
                     /gene="rfbD"
                     /locus_tag="PLK75_06105"
     CDS             complement(10712..11587)
                     /gene="rfbD"
                     /locus_tag="PLK75_06105"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOY38857.1"
                     /translation="MKPTILVCGSEGQLGKCLRDIASQHDYIWHYTDIDTLDITDREA
                     VFQAFSDIKPTLLVNCSAYTAVDNAETDKDAAYTVNAYAVKNLAEACVFFQCRFIHVS
                     TDYVFDGEANCPYTETDRVNPYSVYGASKLEGEQLAAGIDNQSLIIRTSWLYSEYGKN
                     FLKTMIRLGSEKPEVQVIYDQTGTPTYAGDLAVAILTVADRYFHDEQWVPGIYHFSNE
                     GVCSWYDFAVNIMKKQHLHCKVIPIVSAAFPSKVKRPAYSVLSKDKIKTTYHIPVPYW
                     TDSLEVCLKRLGSIH"
     gene            complement(11598..13898)
                     /locus_tag="PLK75_06110"
     CDS             complement(11598..13898)
                     /locus_tag="PLK75_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38858.1"
                     /translation="MALSSKKKKALIITSGIVVLLLLVIVGGIFFLKYKLKKELEKGI
                     SAALSASVSIGKVDIEWFSGKVMLADICVYDKKFPVPKPMLSIAAAGIIADTEKAFNL
                     SNPVIDQIFITGCKIYITTYPNGSSNWDISGARSNGQDSVQKIIINRFDIDDAGIYVL
                     NQQNRKGFRVNGIFLSYEKPTDNVNIQNLYLLIEETALCSGGNSIEFPRVELQGSLSS
                     DSTGYIDISGNVRINQWFSNIDANYDEATGDYSFDWKTPECSIQDVAGIFTPINQKPG
                     QITYTGSFASVLSVKRDSTTCGKPLVTAALNINNAEIINTANNTSIGGYAHCNFEFNP
                     KRRANTFYHNDSILLYSGNDTLHYKLVSNISKNRNENTAITSGSFNLDNLNEIFGHEI
                     YRFSGVISGSNPKSQSPEHALTFRNILVKYGKNPEKSVSLAGELSVNKNQYSLICTVD
                     SNPNALVNLVFSLKTGDNIEDITPITFTALIELPELRIPWQSKSSGTMPDFMTQGKSF
                     KSDFTIPYSTHWINDVSVKIGKVSIDTNNLGALALNYYSDQSKFMCELDVENPESTFL
                     KIKLNSEAIGVNTNRVRLNTSFHFSENEISNTIHGNLSFDADIDAIVNDSLNIAANKM
                     NGKISLLSDSLLIDEKIITDQFSFISGVISSDFLRIRKVAISCNIHNSEFELSDCKAS
                     VDKWKIGMNGVFAPGGNILLKTKLAVNPKELSFVEVQYLKRIAKKNNIDFSQFDKNDY
                     LLIDITSEGKTSEPVNKIKFSSFSRK"
     gene            complement(13925..14260)
                     /locus_tag="PLK75_06115"
     CDS             complement(13925..14260)
                     /locus_tag="PLK75_06115"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOY38859.1"
                     /translation="MKKLVVICVVLLAALNISAQKVYSTQYEYQADVKVYAVQYEYQA
                     DLCVYKVSYEYQAGNNDGKWFFTDYEYQASKKVYFVSSEYQADVKIYFVNYEYQAGWK
                     NKEKMYKFY"
     gene            complement(14260..14601)
                     /locus_tag="PLK75_06120"
     CDS             complement(14260..14601)
                     /locus_tag="PLK75_06120"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOY38860.1"
                     /translation="MKKFIITLTFFMVAFTITSQAQKVYSCDYKSDAQVKVYVCDYKS
                     DADLVVYKCDYKSDASGNEGLWYFCDYKCDADKTIYFCDYKSDADLLIYFSTYKSDAG
                     WQNSSKKHLMY"
     gene            14762..17671
                     /locus_tag="PLK75_06125"
     CDS             14762..17671
                     /locus_tag="PLK75_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOY38861.1"
                     /translation="MRKRIYRVLLMCSPYDAFILEEDGRIDAQLFNEYVSLNLRFPPE
                     IIQVSSASEALEVLEQEKINLIINMLSLIDRDPFELSQEIKEKYNGIPIVVLTPFSRE
                     VNQKLESISGRVFDYVFCWLGDVGILLAIIKLLEDKMNVDYDAKIVGVQTIILVEDSF
                     RYYSSYLPNLYKMLITQAKTYVSEGLNEHEKNIRMRGRPKLLLATNYEEAVALYEKYK
                     ANLLGIITDVRYPRKDKVDREAGFHLLRKIREENSFLPVIIQSSEDDNEDKALRHGAI
                     FLNKNSKTFFFDLKRVILEEFAFGDFIFRHPQTGEEIARVSNIKDLQECIMHIPDEVI
                     VDRARMNFFSRWLKARALFSIARLFEVINTSDFESVTGLKTYIHDTISWYRFNSARGV
                     IAKFYRDEYDRYLLFTRLGDGSLGGKARGLAFIDNLISRNNLRNKYKNLHITIPRTLV
                     VTTEVFDQFMETNKLYEVGLSSLDDETILHKFAEASFPQAFLKDLRKFIAIVKNPLAV
                     RSSSLLEDSHYQPFAGIYSTYMIPYIKDSEMMINMLTDAIKSVYASVYYKESKAYMAA
                     TKNVIDEEKMAVVLQEVCGKKHENTYYPEISGVARSTNFYPIGKEKPEEGIVNLALGL
                     GKYIVDGGMSLRFSPRHPKKILQLSSPEIALKETQNYFLALDLDPKSFKISTNEGMNL
                     LKLNLNAAEMHGVLDNIASTYDYNDQAIHDGVYPGKKIISFANVLKYNRLPLCDAISD
                     VLRIGQSEMSNPVEIEFAVSFDPIDNKKAMLHLLQIRPIVESDEVLPIDVTSIPKENT
                     IITSRSALGNGVIEDVHDIIYVRSENFDPADNPKLAEEIGRLNEQQVLLRRNYILVGP
                     GRWGSSDHFLGIPVKWSQISGVRVLVEMSLQNYQIDPSQGTHFFQNLTSFKVGYFNIN
                     PSIQDGFFDLGFLNRRKPVYESEKLKLIRFAHPAKIYIDGKKGIGLILKPERVLS"
     gene            complement(17768..18430)
                     /locus_tag="PLK75_06130"
     CDS             complement(17768..18430)
                     /locus_tag="PLK75_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38862.1"
                     /translation="MMTGPNYVYKCPNCGNLLKNGSYLSWNNIGAEVFSDGKSITKHP
                     EFPDLTKCKKCDTIFWLSKLEEIVQYEWWRGVKNLNWIMADDAEFLNIEDYFRTLNEG
                     LAENMEEELYIRRQIWWAYNDRVRNGNDPFVDVNDEIKWQENCNKLISLLDESNLEER
                     IMIAELKRNLGDFKSCLEIIKTIDDDELNDDDELNWLKDIFIAECEKGNKWVVKLQPV
                     IE"
     gene            complement(18700..19167)
                     /locus_tag="PLK75_06135"
     CDS             complement(18700..19167)
                     /locus_tag="PLK75_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38863.1"
                     /translation="MKQLIWKLVLPLTIISFLTVTKWWYVDIWGGTDEILTGFPLPFM
                     CRCWHTSLCLQIFVSALIIDVLTYFSFWLTLTFLVNKFLIKIRLNKTLTIILLSLSGI
                     LLVGAIFIGSLSDNIYTWNRNFKFIKLDTGYKFIWDEKYPPDYHEYHPEMKKE"
     gene            complement(19217..20446)
                     /gene="lepB"
                     /locus_tag="PLK75_06140"
     CDS             complement(19217..20446)
                     /gene="lepB"
                     /locus_tag="PLK75_06140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOY38864.1"
                     /translation="MITKNNKDMPDEFNNEKDNLVPEKQVTEKQLKRKSPWLASFLNL
                     FLLGLGHVYAGKIKKGVFIYLGLIFILLSIRFFAYSFHILVGLFAVIICYYLFAIIDA
                     FKSVKRKPYVSPKKYDKWYVYLSIILLQMIFISFLPKGSLNNITPIFFISIPTTSMSP
                     TLQAGDYGTIERTKNIRLNDIVVFKYPQDTNTLFVFRCLSMPGDSLEIINGLAYVNKK
                     LVDNIEKLKFQYFITTNGDAINQRLFDKHGITDYYQVGDYYYLVFITEKEAKELAKNT
                     IVKEINKVTASKDHSLMLFPKSDTICTVDNYGPIYIPQKGKEIKLTRINIGIYASYIQ
                     QENENCLISDSIVKINNQVITDYRFKNNYYFLIGDNRHNARDSRYWGFITEDYIIGKG
                     LYLYWSKKPDRIGCKIE"
     gene            complement(20581..21156)
                     /gene="nadD"
                     /locus_tag="PLK75_06145"
     CDS             complement(20581..21156)
                     /gene="nadD"
                     /locus_tag="PLK75_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOY38865.1"
                     /translation="MHNKLTGLFFGSFNPVHIGHLLIANYFVEFTEMCEVWFIVSPQS
                     PFKQKQHMLPERQRLHLLNLAIESDDRFRASDIEFSMPKPSYTIDTLTYLSEKHPDRQ
                     FAIIMGSDNLTGFAKWKNYNEILRNYRIYVYPRKNTGTGVIHENIVLTEAPAVEISSS
                     FIRDAIASNRDIRHYLPAPVWEYISAMNFFR"
     gene            complement(21146..>21881)
                     /locus_tag="PLK75_06150"
     CDS             complement(21146..>21881)
                     /locus_tag="PLK75_06150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY38866.1"
                     /translation="NKRPLIGDVLDMNLKYTTETDSVLFDSKDAGSDFRMRLEPPAHH
                     GSIEEAFLMMAEGDSAIFKIDGADFYRYTRKLVYVPDFIKPGDKVVFYVRLKKITPKD
                     EFGRQISASDRQYAAEELSMINRFLLTEDLQITPTASGLYFKSLKKQGNIRVKPGNTV
                     SVRYTAMFLDGGVFDAVESNEPPFTFTMGKNEVIAGLEEAVGYMCVGETALAIIPFRL
                     AYGSEKKGIIPPYSTLLFEITVTNAQ"
BASE COUNT         6414 a         4406 c         4340 g         6721 t
ORIGIN      
        1 gatttaattc ggagtttttc cgatgttcct gccgaagtgc ttgctgtgtc gaacggtctc
       61 ctacaccgca actttattac gatcggcttg cttttaaaaa aattattgtt gaagaatgaa
      121 acaaaaatta aaacaaataa tggtctgatt cctgacaatt ggatttatgt gcaggaacct
      181 gaggtcagca tgggtaggat acaggtgttt aataattgga gtccttttat gattgccgat
      241 aattctaaag tctggctcgg actggagtat ttctgcaacc ccgacgatag tctatggact
      301 atggccgata aagatctcat cgaaatggca actgctgaac tcgcaaaaat agatattatt
      361 aaccctgccg atgttcttga tgcaaccgtg ctccgcatgg agaacacata ccctgtatat
      421 tatggcagtt attacgagtt tgatacagtg cgtcgtttta cggataatat tccgaatttg
      481 tttttgattg gtcgcagcgg gatgcaccgc tacaacaatt ccgaccatag tatgatgacc
      541 gctattacag cagtccgcaa tatagttgaa ggccgcaccg acaagtcgaa tatatgggat
      601 gtaaataccg aaagcgatta tcacgaacag aaaaaatgac ccatcagatt ctggttgctt
      661 tccgatttga aaactttttt ttagccattt caatgtaact atgtgtaatt aatcactaat
      721 tttacacaaa acgctctcat atggaacaat catcaataga aaccgcccag cggcaattac
      781 gaactctggc aatcctgcac tatatatggg gaggtttgaa tattgcagga ggcatagcat
      841 tgataataat atacacttca gttggtattg gcatggtggc ggcaggagct atccatggag
      901 aacccgatca gattgcgctg gggatttttg ccggtgtttt tataatgttt gcttttacac
      961 ttgggttttt tataattgct tcagctgttc tctccattgt ttcagcctca tttatgctga
     1021 aacgtaaaaa tcgtgttttc tcaatagtaa ttgccggtac tgagtgtgca aacgcaccgc
     1081 taggcgtact gcttggtgta tttaccatag ttattttgtc gaaacctgag ctcagagagc
     1141 tatataatgc aaataatagt caataaaatc tatataatga aatcagcatt cagtaaacta
     1201 tttgtaggtt tagcaatatt aattattgtt gtaataccaa gttgtaatct ttttgaggag
     1261 agcatacaac ctcctgaaat tcaaactata gaagtaactg aagttacata taacagtgca
     1321 ataagcggag gaaatattac taatgatggc ggagccgatg tcactgctcg tggaatgtgc
     1381 tgggctgttc acacaagccc taccgtgagt gatagcctaa gtaataatgg ttcgggtacc
     1441 ggaatattta ctgctaccct tacagatttg cttccacaga caatttatta tgtacgagcc
     1501 tgggcaacaa atagtgaagg gactgcttat ggcgaggagt ttacgtttac aacgcttcag
     1561 cagcctcttg tgcctagcgt tactacggca ggtgttaccg acattgggga aacaactgca
     1621 gtttgtgggg gcaacgtaac ttccgacgga agttcttcgt tggtagcagt gggtgtttgc
     1681 tggagtacag cagaacaacc gactgtggat gataacaaaa caatcgacgg aacatcaaca
     1741 ggtgaattct tgagtagcat aacagctctg gcacgaaata aaacatatta tgtcagggcc
     1801 tatgcgacca atagtcttgg aacaggatat ggagaggaat attcttttac tactttgccc
     1861 agtcctacag ccggcactgt taccgatatt cagggaaata cctatactac cgatgtcttt
     1921 ggaactcagg tgtggatgac ccaaaacctg cgcacaatcc gttacaacga taacaccgat
     1981 gtaccgataa taacggatga tgcccaatgg agcaatttga gtacgggagg tttttcggtt
     2041 ttcaacaatg atatagataa ttactacggc ttatatggcg cactttacaa ctggtatgcg
     2101 gtgcagacag ggaagctttg cccttcaggc tggcacgttc ctaccgatga tgagtggaaa
     2161 acattggaga tgaatctggg tatgagtcag actgaagccg atggcagcgg taccagaggt
     2221 actaccgagg gtggcgaaat gaaggaatcc gggctggaaa actgggctta ccccaatacc
     2281 ggtgctacca atgaaagcaa tttcacaggt ttgcctgcgg ggatacgaag ggtaaacggt
     2341 gtgtatgccg aaaacagaac agccgggtat ttctggtcag ctactgatgg cggggaactc
     2401 aacgcatggg cgcggctttt aagcaatagc aacagcaatg tttcgcgagg gcaatactat
     2461 aagcagttcg gtatgtcggt gcgctgtgtt caggattaat gaaaatctgc ttttcaatta
     2521 cgcgtctttt cttccaaaac cctgacgata caacgattct ataattacaa tctctgcttc
     2581 gtcattcaga ttatttccaa taggttgcaa taattcgggg aaattattac gtttgttttt
     2641 aactgaactt ccaatagggt aggcattcat tttgttgctg tcgtatggtt ttaacatggc
     2701 ggtgatatca gaaagaggag catcattatt cagccagaca gactcctgcc atggttcgag
     2761 tataacagga gaacgatggt gtggtatctt ttgcattagc tcattggcaa ctgtagttat
     2821 aattgcaaac gaaaaaaccg attcaccgtt aacagggttt ttccattcat cccatatgcc
     2881 tgccatggca aatggtcgaa ctttgttttt taagaataca acatgcgctt tatcgagacc
     2941 ttcatcagtt gaaccttcga taaatgcatc ggctattacc aggcagcgct gagagcggat
     3001 aggtttgcga aatgatggtt ttagaataat atcctttggc ccgtgatagt gaacatcatt
     3061 ttcggagttc ctgtcgccct cggtgcgtgc attgagcaga aacatatcct tttttgccca
     3121 cgacgggcac atcccgaagc ggaaaagctg cagacatcgc ttactattgc ttgcaacaac
     3181 cggtgtgtac atccccggcc ccgtgttata cgatggcgat aagtcgaaaa gagagggcga
     3241 tagattaaat ctcttttcga tggtttcgag tttttgcacc agaatgaagc gaccgcacat
     3301 tgctattgaa cttgtttatt gcaaaattac aatattttac aatatcattt tttttatgga
     3361 cggggtgatt caattctttt gaatctggtt gcggagaaat tcctttgttg gcaccagaat
     3421 attaacgcta tatttcagta atttcatttg atatcagtat tacacaaata acatgatttg
     3481 ttttatgaaa agatgtaaac atcggtttta ataaaattag caattgtcta tatttgtgcg
     3541 aatagaaaaa agtatggata attttattgt atcggcacga aaataccgcc ccgataattt
     3601 cgattcggtt gttggtcagg atagtataac ccgaaccctg aagaattctg ttaaaacgag
     3661 gcaattggcg caggcgtatc ttttctgtgg acctcggggt gttggaaaaa caacctgtgc
     3721 acgtattttt gcaaagacaa taaactgctc aaatccaact cctgaacatg aagcatgcaa
     3781 tgtgtgtgaa tcgtgtaaaa cctttaacga gtcgcgatcg cttaatatcc atgagcttga
     3841 tgcagcatcg aacaactctg tggatgatat ccgaaacctg attgatcagg tacgcatccc
     3901 gcctcagatt gggaaattca gcgtatatat cattgacgag gttcacatgc tcaccagctc
     3961 agcattcaat gcattcctta aaacgctcga agagcctccg aagcatgtgg tgtttattct
     4021 ggcgactacc gaaaagcata aaattatacc taccatttta tcgcgttgtc agatattcga
     4081 ctttaatcgc atcagcattc atgatatcac agcgcgcctc aagtatgttt cccaaaatga
     4141 aggaattaca gccgaaacag atgcattgtc gataattgcc cagaaagcag atggtgctat
     4201 gcgtgatgct ctttcgatat tcgatcagat agtaagcttt tcgggtaaca atattaccta
     4261 tcaaaacact atcgaaaacc taaatgttct cgattatgag tattatttta gagttacccg
     4321 tgtgttactg gagcacgact ataggggagc tctgaatatt ttgaacgaag taattgaaaa
     4381 tggttttgat ccgggtgtgt ttttatcggg gttggcatct catttacgcg atattcttat
     4441 gtgtaaagac cccggaacct acaagctgct cgaggtaggt gaaaatatac agaaaaaata
     4501 ccacgagttc tcaggaatct gcgatgtgga ttttttgtat aaggcaatgg atattgctaa
     4561 tgaatcggat tttaagtata aagtcacccg caataaacgc ctgcttgtcg aaatttgcct
     4621 gatgaaaata tctttcatca atcatcagtc aaatataccg gttaaagaaa gcagacctgt
     4681 ggtaacacag gaaccttcga aatcttaccc ggcaacggga acagcccctg aaagacccat
     4741 tgaaaataag tcgaatgatg aaaagcagga acagggtcct gcagttcagc caaacgaatc
     4801 gcacaatgaa ggaacagtca ttaaaaccga tgtctcaccg aaagaagaac cagggtttcg
     4861 taatggcaga attccatcag tatcaattaa aaatatagga aaaaacctaa cagcacagca
     4921 gtcggaacct caaacttatg aggctaatga ttccacaagg gttatcgtta ttggtgatga
     4981 cgaattcaac acggcaaagc tattggacaa atggcaggag tttactgcaa aactatctga
     5041 caagcctcgt ctttttcagc tattcaacaa cgccaatcct gtgttgaatc cagattgggt
     5101 aataggcctg gaaattgata atcagaatct ctacgatctg gttatgtcaa taaaacccga
     5161 attgctgttg tatttgcaaa ccgcactgaa taataagaat atcagccttg acttacaaat
     5221 tgctgaagcg ccagataatt ccggtgaatt gctatatacc gatgaagaaa agattcagta
     5281 tatggtaagt aaaaagcccg aattggccac tttaataaag caacttgatt tggatttata
     5341 attatcgcct acgattaaag gacagtattt agatgggttt cggataaata tctgacaaaa
     5401 tcatcggata ctttgtttgc tatttgttga agaatacatt tccccattac acaatttgcg
     5461 tgatcgaaag ggcaggatgt taatgctatt ttaccttcaa tggcttcgta aatttcgagc
     5521 agggtgatgt tgtcggcact tttctttaga cggaatccac ctgaaggtcc acgaaccgat
     5581 gaaattagcc ctgatttcac caagcgttga aggatttttg ccacatgatg cctgctcgat
     5641 tccgaaatat tagcaatttc ggttgcatta atgctgtttt cggagcgggc aatcaacacc
     5701 atactatgca gggcaatacc tgctgcttct gaaattgcaa taacctttga catagcaggt
     5761 caaaatttca tttcctgaag ctcgacgttt ttaacttctc tgatacgttc caggaaaagt
     5821 tttttttgct tttcgtcgcc atgtaggtca acaattatta ttcctcctga aatttcatcg
     5881 ttgtttattc caaggcgggt tctaataaca cagccaaagc ttgtcataat tttttgtact
     5941 tcctggaatg caacagaggg atcctttata aagatacctg caatttcata ttcgttttgc
     6001 ataatgttat ggtttaaaga ccattttttt tacttcgatt ccgccgatat ttgcgaggtc
     6061 gcttacaagg gcctcgcgat cagccttatt ccctttgagt tctataataa ttaatgcatt
     6121 gcgactgcat ttgtaatcac tcacttcgtg aaaacccagc cgggtacgca aaacattacc
     6181 gtgttgcgaa agaactcttt gtgttttccc cgcttcttta atccggtcga gcacatgaat
     6241 gccaattatt gtaatatcgc tcataatgat gacttaaaaa tataaatctc tttttccatt
     6301 tttaatttgt tcgattctat tcttcaactc gggtaggttt ttgtaatcgc cgagctttac
     6361 taattctgtc tcaatcagct tccaaccttt ttcggcaact tctggagttg catagtcgca
     6421 cagatactcc gagagagtta aaatagcgtt gggagtgcag tatcttttaa taaagccggg
     6481 tacactgaat tccatgaaat gttcgcctgt acgtcctttg cggtaacaag cagtgcaaaa
     6541 tgacgggatg aagtttttgc taagcagttc gtcgataatt tcacccaatg agcgtgtgtc
     6601 gttaatggca aactgctctt tgttcaaatc ctgtttttcg ttcaggttgg cagaatagct
     6661 tcccagctcg agtttggtgc ccccgtcaat ttgtgacaca ccaaaactga ttacttcatc
     6721 gcgaactcgt gccgattcac gggcagtaag aattaaaccg gtatagggca cagcaagacg
     6781 gagaattgca ataatcctta caaaagcatc atcattaacc tcatacattg gatcgaggtt
     6841 taaccccaat gcactcttaa tacggggaaa cgaaatagtg tggggtccaa cattatagca
     6901 ggcctccaga tgattggtat gccgtaccag cgccattact tcgaatcgcc agtcgtataa
     6961 tccaaataaa gcaccgatac ccacatcatc cacaccggct tcctgagccc tgtcgagccc
     7021 tgtgagacgg taattgtaat cacgtttttt ccctccgagg tgatattttt tataagcttc
     7081 gggatgatag gtctcctgaa atatctgata cgtccctata cctgcttcgt gaactatcct
     7141 gaatccatct atttcgagag gagcggcgtt aatattcacg cgcctgattt caccattttt
     7201 ctttttaaca ccgtatgcaa tcctaacagt atgagcaata tattccggag aataatcagg
     7261 atgttcacca tatacaagga tcagtctttt ttgcccgttg tcttcgagtg cttcaatctc
     7321 tctcacaagt tcttcgtcgg taagggtttt tctgatggct tccttattcg atgctttaaa
     7381 accacaatac tggcagttgt ttgtgcattt gttaccgatg tatagtgggg caaacagtac
     7441 aatcctgttt ccatacactt tttctttaag gcgcagtgcc ccatttttaa tttcctgaat
     7501 cagttcggca ctgtcggcat ttatcagaac cgcagtttct tcgagactta atctctcttt
     7561 attaaggctt ttctcaatca cttcgcgtac gcgcaaagca ctgggatttt ggttttcttt
     7621 caggatcctt tcaatttctt cggggtcgat gaaaggctgc attctcttat cgggaatgtt
     7681 gtactgttgt ggtttgaaaa tcatggaata gaaaattaaa ctcaaataaa aaaataaaac
     7741 cagatattgt tgcaaaaaaa actatctggt ttacggttta ttcttcgcgc atcctgtaaa
     7801 cgtccctgtc gaacatgtat atagcacctg catttttccc caacaggctt aaacgatcga
     7861 gaatggcatt tacattggct tcttcttcga gttgctcagt tacaaaccac tgtatccagt
     7921 tgttggttgc aaaatcgttc tctttaacgg tcagggcaac tattttatgg atgctttcag
     7981 tgatatatat ttcatgtttt agcacctctt cgaaaagctt atgaatgtca gtccattttt
     8041 caggtgcctt ttcaattgcc ggaataatgg ctttaccacc acgctcatta atgtaatgaa
     8101 caaacttaag catatgctct ttttcttctt cagcctgagc atagagccac ttagctgttc
     8161 cactgagctt gttattttcg gcccatacag ccatcgataa atacaaaaat gacgaatagc
     8221 cttcttttac aatctgttcg ttcagaattt tttcgatact tggttttaac ataattacta
     8281 aatttaatag ttaataattt ataacaaaag taaggattaa atgtttatga tattctaagt
     8341 aaatttagaa aagctttact atttttaaaa aaatcaaaat cattatggat aaaatcaaaa
     8401 taatccggtt cgatgaagtt gagtcaatac tgaattcggt gcagatttgt acccttgcga
     8461 tgatagataa tggcaagccc tatgctgtat cgatgaattt cggttatatc gacagaacaa
     8521 tctattttca cggagcacct tcagggcgaa aaattgatgt gctgaacatg aataaaactg
     8581 tgagtgcagt tttttatacc gatgcagtgc tgaatgttag gcacgaaaca gtagcctgtt
     8641 cttattccat gaaattccgg tcggtagttg ttaatgggga aattgaattc gtaaccaaca
     8701 atgatgagaa aataattgca ctgaattcag taatgcaaaa gttcagcgac agaaccaact
     8761 ttacatatgg cttacccgcg ctgaacaacg tgaatgtgtt caaactgcct gtaattgatt
     8821 acactgcata taaaagagga tattgatagc taaaaaatat ctttcagttt acacaggtgg
     8881 tttatctcat aagtaaccgg acggttggct gttttgttga cagtattaaa ccaaactgta
     8941 tcaagcccaa ttgtattcgc ccccttaata tctacctcca aatcatcgcc aattacaacc
     9001 gaatcggcaa cagatgctcc tgttttttga agcacaatct caaaatattc tttttctggt
     9061 ttgttgaatc caatttcttc actggtaaac acctgctcaa aataggggcg cagttgacag
     9121 ttttcaattt tgcgatattg aacttctttg aaaccgttag taattaggaa cagtttatat
     9181 ttgtgctgta ggtacgcaag aacttcgtgc gtacatggga aaagcctgct tttatcagga
     9241 ctatgctgaa tataatcggt tcccatattc tttgccatta attcgttaaa aatacctttc
     9301 tcttcaagcg tcatctgaaa tcgttgccaa ctgatatcct gctttgtaat tatatgagaa
     9361 cggtactgat tccatagaaa gtcgttgtgc ttgcgatata tagcatgaaa ctcgtcgaaa
     9421 ctttcaaaat gcttgccaag cgggtacttt tcgaaaattt cgcgcagggt ttctgttgag
     9481 tttgtttcga aatcgtagag ggttctgtcg agatcgaaaa ataaatattt atattttttt
     9541 tgtattgttt ccatcgtcag ataaaatgcc tgcaaaaata aaacttcaat gcatacaaaa
     9601 catcggtaaa agtcataata ttattaaaat cttaaaaaag tgtaaatttt taccaatttc
     9661 gctgtattga atagttgata atgcaactgc catgttaagt gatagcaaca attacaatgc
     9721 ctgagtaaat acattttata cagtaatatt tttgtgtttg caataattct tttttgtttt
     9781 agtacctttg ggttttaatg aatgcaagtt aaagcgtaag cgaaatgaaa aaaattgccg
     9841 attcagaatt gatattaaac acagatggaa ctatttttca tctgcatctt cttccgcatc
     9901 agatagccga cgacatacta ctcgtcggcg atcagggtag ggtcgatatg atttcgaaac
     9961 acttcgaaaa gttggaaaca agagtgcaaa accgcgaatt tgttacacaa accggagttt
    10021 acaatgggaa acgaataacg gtaatatcaa caggtattgg cacagacaat atcgacatcg
    10081 ttgtaaacga actggatgct ctggtaaata tcgatatgca aagcaggaca gaaaaacctg
    10141 aaaaaaaatc attgaatttc atacggattg gcacctcagg aggaatgcaa ccctatctga
    10201 atgccggcga ttttcttgtt tccgatactg ccatcgggtt cgatggattg ctcaattttt
    10261 accacgattc gcaacgggta tgccgcagcg actatgaaga atcgctgatc agttatctga
    10321 actatcatac tgcattaccc agaccttatg tggttgatgc cgataaaact atgattgaca
    10381 tgctgggaga gggcttgcat catggtgtaa ccatttcggc tcctggtttc tatggtcctc
    10441 agggcagaga attgcgtctg ggacttgtcg atcccgatat taactccaaa atatctgctt
    10501 atcgttttgg aaaggaaaaa attaccaact acgagatgga aagctctgcg attttcggat
    10561 tgtcgaaaat gctcggacac aaagctgtaa cagtgtgtgt aattattgcc agccgtttca
    10621 ataaaactgc actgcccgat tacaaaccca aagttgagga gcttgtccaa ttggtgctgg
    10681 acagaattac tggccgataa ctccggagct attaatggat actgcctaat cgtttaagac
    10741 aaacctcaag actgtcggtc cagtagggta cgggtatgtg gtatgtagtt ttaattttat
    10801 ccttgctcaa cacactgtat gcagggcgtt ttaccttcga ggggaaagct gctgaaacaa
    10861 tgggaattac tttgcaatgg agatgctgct ttttcatgat gtttaccgca aagtcgtacc
    10921 aactacaaac cccttcgttg ctgaaatgat atattcccgg aacccattgt tcatcatgaa
    10981 aataccggtc tgcaaccgtt aatattgcta cagcaaggtc gccggcatag gtaggcgttc
    11041 cggtctgatc gtagataacc tgtacctcag gcttctcaga tcccaaccgg atcatggtct
    11101 taagaaagtt cttcccatat tctgaataca gccacgaagt tcggataatt aacgattgat
    11161 tatctattcc agcagcaagt tgctcacctt caagcttcga agcaccataa acactgtatg
    11221 ggtttaccct gtcggtttcg gtgtacggac aattggcttc tccgtcgaaa acataatcgg
    11281 ttgacacatg aataaacctg cattgaaaaa aaacgcatgc ttctgcaagg ttttttactg
    11341 catacgcatt caccgtgtaa gctgcgtcct tgtcagtttc agcattgtcg accgccgtat
    11401 aggcagaaca attcaccagc aatgtcggtt ttatgtcgga aaacgcttgg aatactgctt
    11461 ccctgtcggt tatatcgagc gtatcaatat cggtgtaatg ccaaatataa tcatgttgag
    11521 aagcaatatc tctgagacat ttcccgagtt gcccttccga gccgcaaacc aaaatggtgg
    11581 gtttcatagt attgtcttta tttacgtgaa aacgatgaaa atttaatttt atttacgggc
    11641 tcacttgttt tgccttcgct tgtaatatca atcaaaaggt aatcgttctt atcgaattga
    11701 ctgaaatcga tattattctt tttggcaata cgctttaaat actgaacctc aacaaaactt
    11761 aattctttcg gattaactgc cagttttgtc ttcagcaata tattaccacc gggcgcaaac
    11821 accccgttca tgccgatttt ccatttgtca acagaggctt tgcagtcgct caattcaaat
    11881 tctgagttgt gaatgttgca gctaatggct actttcctta ttctcaaaaa gtcggaagaa
    11941 ataacaccgg aaataaaact gaattgatcg gtaattattt tttcatcaat taacagactg
    12001 tcgcttaata gtgatatttt accgttcatt ttgttcgcgg caatatttaa tgaatcgttt
    12061 acaatagcat caatatcagc atcaaacgat aaattgccat gaatggtatt tgatatttcg
    12121 ttttcagaaa aatgaaacga tgtattgagt ctgacccggt ttgtgtttac gcctattgct
    12181 tcgctgttca gtttgatttt caggaaagtg ctttcaggat tttcgacatc cagttcacac
    12241 atgaacttgc tttgatcgct ataataattc aatgccaatg ctcccaaatt atttgtgtca
    12301 attgatactt ttccaatctt cacactaaca tcatttatcc aatgcgtgga atagggaatg
    12361 gtaaaatcag atttgaatga ttttccctgt gtcatgaaat cgggcatggt gcctgatgat
    12421 ttgctttgcc agggtattct cagttcaggg agctcaatca gggctgtaaa cgtgattggt
    12481 gtaatatctt caatattatc gcctgttttc agtgaaaaaa ctaaattgac caaggcattg
    12541 ggatttgaat caacagtgca aattaagctg tactggtttt tgtttaccga taactccccc
    12601 gctaacgaaa ctgatttttc gggattcttt ccatacttaa ccaaaatatt tctgaacgta
    12661 agagcatgtt cgggtgactg tgattttgga ttgcttccag atatcacacc tgaaaaacgg
    12721 tagatttcat gcccaaaaat ttcgttcaga ttatccagat taaaactccc cgaagttatt
    12781 gcagtatttt cattccggtt tttcgaaata ttgcttacca gcttgtagtg cagcgtgtcg
    12841 ttgcctgaat agagcaaaat actgtcgtta tggtaaaatg tgtttgctcg tcttttcgga
    12901 ttaaactcaa aattacaatg tgcatacccg cctattgagg tgttgttcgc tgtattgatt
    12961 atttcggcgt tattgatatt caatgcagca gttaccagag gcttgccgca agttgtgcta
    13021 tctcgtttta ctgataatac tgaagcaaat gaccctgtat atgtaatttg gccaggcttt
    13081 tggttgatag gggtgaaaat accggcaaca tcctgaatac tgcattcggg cgttttccaa
    13141 tcgaaagaat aatctccagt ggcttcatcg tagtttgcat cgatatttga gaaccattga
    13201 ttaatgcgaa cattaccact gatgtcaata taacccgtgg aatcgctgct taatgagccc
    13261 tgtaactcaa cccgcgggaa ctcaatgcta tttccgccgg aacacaaagc agtttcttcg
    13321 ataagcagat acaggttttg gatattaaca ttatcggtcg gtttttcata acttaaaaaa
    13381 ataccattta ctcgaaaacc tttcctgttt tgttgattca acacataaat acctgcatca
    13441 tcaatatcga aacggttaat gattattttt tgaacagaat cctgtccgtt tgatcttgcc
    13501 cctgaaatat cccagttcga actgccgttg gggtaagttg taatgtatat tttgcagcct
    13561 gtgataaaaa tctggtcaat tacggggttc gataagttga aagctttctc ggtgtcggca
    13621 ataataccgg ctgctgcaat cgaaagcatt ggtttcggga cgggaaattt tttatcgtaa
    13681 acgcagatgt cggcaagcat aaccttacct gaaaaccatt ctatatcaac cttgccgatg
    13741 ctaaccgacg ctgacagcgc agcagaaatg cctttttcga gctctttttt tagtttatat
    13801 ttcaggaaga aaataccgcc cacaataacc agcaatagca gtacaacgat accggaagta
    13861 atgatgagag cttttttttt cttactcgaa agagccatcc ctgccttaaa attattgccc
    13921 cgatttaata gaacttatac attttctctt tgttcttcca accggcctga tattcatagt
    13981 ttacaaaata tatcttaaca tcagcctgat attcgctgct tacaaagtac acttttttac
    14041 tggcctgata ttcataatca gtgaaaaacc atttgccatc gttgtttccg gcctgatatt
    14101 cgtaacttac cttatacaca cataaatcgg cctgatactc atattgcact gcatacacct
    14161 taacatcagc ctgatattca tattgagtgc tgtacacttt ttgggcacta atattaagag
    14221 cagccagcaa cactacacat attacaacca gctttttcat cagtacatca ggtgtttttt
    14281 tgatgaattc tgccaacctg catcactctt atacgttgaa aaataaatca gcagatcagc
    14341 atcacttttg taatcgcaaa aataaatggt tttgtcggca tcgcatttgt aatcgcaaaa
    14401 ataccacaaa ccttcgttgc cgctggcatc gcttttatag tcgcatttgt aaaccaccag
    14461 atcagcatcg cttttatagt cgcacacata cacttttacc tgtgcatcgc ttttataatc
    14521 gcagctgtat actttctgtg cctgcgatgt aatggtgaag gctaccatga agaaggtgag
    14581 agtgataata aattttttca tgactgtatg tattatttcg taaatatata taaattaaca
    14641 caaaaattga accatgcccg acgattcttt aaaacaaaat acaaatattc ataaagataa
    14701 gttttatcag agcgataatc ttgataatta ctattttgag cggacagcct tcaataagct
    14761 gatgcgtaag cggatttatc gtgtgctgct gatgtgcagc ccttacgatg cttttattct
    14821 ggaagaagac gggcgtatcg atgcccagtt gttcaacgaa tatgtttcac tcaatttgcg
    14881 ctttcctccc gagattatcc aggtatcttc ggcatctgaa gcgctcgaag tgctggagca
    14941 ggagaaaatc aacctgatta tcaatatgct cagccttatc gaccgcgatc cgtttgaact
    15001 ttcgcaggaa attaaagaaa agtacaatgg cattcctatt gtggttctta caccgttttc
    15061 gagggaagta aatcaaaagc tggagtcgat aagcggcaga gttttcgact atgttttctg
    15121 ctggcttggc gatgtgggta ttttacttgc aattatcaag cttctcgaag ataaaatgaa
    15181 cgtagattac gatgctaaaa ttgtcggcgt tcaaaccatt atccttgttg aagactcatt
    15241 tcgctattat tcaagttacc tgccgaatct ctataaaatg ctcatcacac aagccaagac
    15301 ctatgtatct gaaggtttga atgagcatga aaagaacatc agaatgcgcg gcagacccaa
    15361 gcttttactg gcgaccaatt acgaagaggc agttgcactg tacgaaaaat acaaggcaaa
    15421 tctgctggga ataatcaccg atgtcagata cccgcgaaaa gataaagttg acagggaggc
    15481 cggcttccat ttattgcgta agattcgcga ggagaattcg tttttgccgg ttatcattca
    15541 gtcgtcggaa gatgacaacg aagataaagc attgcggcat ggcgccatct ttttgaataa
    15601 aaactcgaag acattctttt tcgacctgaa aagggttatt ctcgaagagt ttgcctttgg
    15661 cgactttata ttcaggcatc ctcagacagg ggaggagatt gcacgggttt cgaacattaa
    15721 agacctgcag gagtgcatta tgcacatccc tgatgaggtg atcgttgacc gtgctcgcat
    15781 gaacttcttc tcccgatggc ttaaagcccg tgccttgttc agtattgcac gtctcttcga
    15841 agtaataaac acatccgatt tcgaaagtgt aaccggactg aagacctata ttcacgatac
    15901 catttcatgg taccgcttca acagcgcgcg cggtgtaatc gccaaattct accgtgatga
    15961 atatgaccgc tacctgttgt ttacccggct gggtgacggt tcgctgggag gaaaagcaag
    16021 ggggctggca tttatcgaca acctgatatc gaggaataac ctccgcaaca aatataaaaa
    16081 tttgcatatc accatacctc gtaccctggt ggttactacc gaggttttcg accagtttat
    16141 ggaaaccaat aagctctacg aagtaggatt atcgagcctc gacgacgaaa ccattttaca
    16201 caagttcgct gaagcaagct tccctcaggc ttttctcaaa gatttacgca agttcattgc
    16261 cattgtaaaa aacccgcttg cagtaaggtc ttcgagtttg ctcgaagaca gccattatca
    16321 gccttttgct ggtatctact ctacctacat gatcccgtat atcaaggatt ctgagatgat
    16381 gatcaacatg cttaccgatg caatcaagag tgtgtatgct tcggtgtatt acaaagaaag
    16441 taaggcatat atggctgcaa ccaagaatgt gatcgacgag gaaaaaatgg cggttgtgct
    16501 gcaggaagtt tgcggtaaaa aacacgaaaa cacatattat cccgaaattt cgggtgttgc
    16561 aagatctacc aatttttacc ccattggcaa ggagaaaccc gaagaaggta ttgtaaatct
    16621 ggcattgggg cttggaaaat acattgttga cgggggaatg tcgctgcgct tttcaccacg
    16681 ccacccaaag aagattttac agctcagcag ccccgaaatt gccttgaaag aaacccaaaa
    16741 ctattttctg gcgctcgacc ttgacccgaa aagctttaaa atatccacca acgaaggtat
    16801 gaacctttta aagctcaacc tgaatgctgc cgaaatgcac ggagtgctag ataacattgc
    16861 ttccacctac gattacaacg atcaggccat tcacgacggt gtgtatccag gtaaaaaaat
    16921 tatcagtttt gccaatgtac ttaaatacaa tcgtttacct ctgtgcgatg ccatctccga
    16981 tgtgctgcgt atcgggcaaa gcgaaatgag caatccggtg gaaatagaat ttgccgtttc
    17041 gttcgatcca atcgacaaca aaaaggcaat gctgcacctg cttcagatac gacccattgt
    17101 tgaaagcgac gaagttctgc caattgatgt taccagcatc cccaaagaaa atacaattat
    17161 aaccagccga agcgccttgg gtaacggggt tatagaagat gttcacgaca ttatctatgt
    17221 gcggtcagag aatttcgacc ctgccgataa cccaaagctt gccgaagaga ttgggcgtct
    17281 caacgaacaa caggtgctgc tccgtagaaa ctatattttg gtcggtcccg gacgatgggg
    17341 ctcaagcgat cactttttgg gtatccctgt gaaatggtcg cagatatcgg gtgtgcgtgt
    17401 gctggtggaa atgtcgttgc agaattacca gatcgacccg agccagggaa cccacttttt
    17461 ccagaacctg acctcgttca aggtaggata tttcaacatc aaccccagca tccaagacgg
    17521 atttttcgac ctgggtttcc tgaatagacg caaaccggtg tacgaatcag aaaagctgaa
    17581 actgatacgc tttgcacatc ctgcaaaaat ttatatcgac ggaaaaaagg ggatagggct
    17641 tattctgaaa cctgagcggg tgctgagtta agggtaaggt atgtgtactc ttggacataa
    17701 tttctgaacc tgcatctgcg tattcgcaat agggtatgaa tgctttagaa tttactggct
    17761 gggcagtcta ctcgattaca ggctgtaact ttacaaccca tttgtttcct ttttcacatt
    17821 cagcaataaa tatgtctttc aaccagttta gttcatcgtc gtcattaagt tcatcgtcgt
    17881 caatcgtttt aattatttca aggcaacttt taaagtcgcc taaatttctt tttaattccg
    17941 ctatcataat tctttcttct aaattggatt cgtcaagaag cgagattagt ttattacagt
    18001 tttcttgcca cttgatttcg tcattcacat caacaaatgg gtcattgcca tttcttactc
    18061 tgtcattata agcccaccaa atttgtcgac gtatatataa ttcttcctcc atattttcag
    18121 caagtccttc attaagagtt ctaaagtagt cttcaatatt tagaaattca gcgtcatccg
    18181 ccattatcca atttaaattt tttacgcccc tccaccattc atattgcaca atttcttcca
    18241 atttactcaa ccagaagatt gtatcacatt ttttacactt agttagatca ggaaattcag
    18301 gatgcttagt aatactctta ccgtcagaga atacttctgc accaatattg ttccaactca
    18361 aataacttcc atttttcaaa agattgccac aatttgggca tttgtaaacg taatttggtc
    18421 ctgtcatcat attgtattat attttaatgg cttatgcgag tcgctaaccg cacctgcgtt
    18481 ggttattttt taattagtca tagggttgat aattcactaa actagtgaaa ttcaatttca
    18541 aagttacaaa aaatataatt atgtgaacac gtaattacct gaaaaatttg ttgcagaaaa
    18601 attttcacaa acattcgagt tattactaac acaccagcag aagtatgtta atgcttttga
    18661 accactggcg gggcagttaa cccgattttg caagggtttt tattcttttt tcatttctgg
    18721 gtgatattca tgatagtctg gtggatattt ctcatcccaa atgaacttat agccagtgtc
    18781 caatttaata aatttaaaat ttctattcca ggtgtaaata ttatcggata gacttccaat
    18841 aaatatcgca cctaccaata aaattcctga tagagataaa agaattatag tcaaagtctt
    18901 attcaaccta atttttatta gaaatttgtt tacaagaaaa gtaagcgtta accaaaaact
    18961 gaaataggtt aaaacgtcaa ttattaaagc tgaaacgaag atttgtaagc agagagaagt
    19021 atgccagcac cgacacataa atggtaaagg aaatccggtt aatatttcgt ctgttccacc
    19081 ccaaatgtct acataccacc acttcgtaac agttaaaaac gaaataatcg tcaagggcaa
    19141 tactaatttc cagatcaact gtttcatttc ttttttttaa ctaatgcttg cagcaaaacg
    19201 ctgtatatac cagcttttat tcaattttac atccaattct atctggtttt ttactccaat
    19261 aaagataaag acctttccca atgatataat cttctgtgat gaaaccccag tatcttgaat
    19321 cacgagcgtt gtgccggttg tcaccaatga ggaagtaata attattttta aacctatagt
    19381 ctgtaattac ttgattattt attttaacta ttgaatctga aattaagcaa ttctcatttt
    19441 cctgttgtat gtatgatgca taaattccaa tgttaattct agtcaactta atttctttcc
    19501 ctttttgagg gatataaatt ggtccgtaat tgtcaactgt gcaaatcgta tctgattttg
    19561 gaaaaagcat taaagaatga tccttgcttg ctgtaacttt attaatttct ttaacaatag
    19621 tgttttttgc caattctttc gcctcttttt ctgttataaa tactaggtaa tagtagtcac
    19681 ccacttgata atagtcagtt attccatgct tatcaaataa cctttgattt attgcatctc
    19741 catttgttgt gataaagtac tggaatttta gtttttcaat gttgtcaacc aattttttat
    19801 tcacataagc aagaccattt atgatttcca aactatcacc tggcatgctc agacatctga
    19861 aaacaaatag tgtatttgtg tcttgtgggt atttaaaaac aacaatatca tttaatctta
    19921 tatttttagt cctttcaatt gttccataat cacctgcctg caatgttgga ctcattgaag
    19981 ttgtcggtat ggagataaag aaaattggtg taatattatt aagacttcct tttggtaaaa
    20041 aacttataaa tatcatttga agtaaaataa ttgataagta gacataccat ttatcatact
    20101 tttttggaga aacatatggc tttcgtttta cagatttaaa ggcatcaata attgcgaata
    20161 ggtaataaca aattataact gcgaatagac caactaatat atgaaagcta tatgcaaaaa
    20221 acctaataga taataaaata aatataagcc ccaaataaat gaatactcct ttttttattt
    20281 tcccggcata aacatgaccc aagccaagaa ggaataaatt taagaatgat gcgagccaag
    20341 gactttttct ttttagttgt ttttcagtga cctgtttttc cggcactaaa ttatcctttt
    20401 cattgttgaa ttcatcaggc atatctttat tgttttttgt tatcatattt aattggtgct
    20461 actaagtcgc taaccgcaac tgtggtgttt atttttgaat tagacttagg tttgataatt
    20521 cacaaaacta ctgtaatcca ctttcaaagc tacaaaaaat aaaattatgt gaacacgcaa
    20581 ttacctgaaa aaattcatag cagaaatata ctcccaaaca ggtgccggca gataatggcg
    20641 gatatcacgg ttcgaagcaa tggcatcacg gatgaacgag gatgaaatct ctacagcagg
    20701 tgcttcggta aggacgatgt tttcatggat tacaccggtt ccagtgtttt ttcgcggata
    20761 tacataaatg cggtaattcc tgagaatttc gttgtagttc ttccatttgg cgaatcctgt
    20821 caggttgtcg ctgcccatga tgatggcaaa ttgcctgtcg ggatgttttt cgctgagata
    20881 ggtgagcgtg tcgatggtat aacttggttt tggcatcgaa aattcaatgt cggatgccct
    20941 gaagcggtcg tcgctttcga tggcaaggtt cagtagatgc agccgctgcc gttcgggaag
    21001 catgtgttgt ttttgcttga acggactttg cgggctcaca ataaaccaca cttcgcacat
    21061 ttcggtaaat tccacaaaat aattggctat caacagatgc ccgatatgca ccgggttgaa
    21121 ggaaccaaaa aacagaccgg tgagtttatt gtgcattggt tacagttatt tcaaacaaca
    21181 atgtggaata gggggggatg atgccttttt tttctgaccc gtaggcaagc ctgaaaggta
    21241 ttatcgccag tgcagtctca ccaacgcaca tatagcccac agcctcttca agaccagcaa
    21301 taacttcgtt ttttcccatg gtgaatgtga aaggtggctc gttgctttca acagcatcaa
    21361 aaacacctcc gtcaagaaac atcgctgtat agcgcacact aaccgtattt cccggtttca
    21421 ccctgatgtt gccctgtttt ttcaggcttt tgaaatagag tccgcttgca gtaggggtta
    21481 tttgcaaatc ttcggtcagt aaaaagcggt taatcatgct cagttcttca gcagcatact
    21541 gtcggtcgct tgccgagatc tgccttccga attcatcttt cggcgtaatt tttttcaatc
    21601 tcacgtagaa caccacctta tcacccggtt ttatgaaatc gggtacatac acgagttttc
    21661 gggtatagcg gtaaaaatcg gctccatcaa ttttaaaaat tgcactgtcg ccttcagcca
    21721 tcatcaggaa ggcttcttca atgctcccgt ggtgggccgg gggctcaagc cgcatcctga
    21781 agtcgctccc tgcatctttc gaatcgaaaa gaacagaatc ggtttcggtg gtgtatttta
    21841 agttcatatc aagaacatcg ccaatgagag gccgcttatt g
//
LOCUS       DAOOQP010000042        21764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_2944_length_21764_cov_4.927772, whole genome shotgun sequence.
ACCESSION   DAOOQP010000042 DAOOQP010000000
VERSION     DAOOQP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21764
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_21764_cov_4.927772"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1046)
                     /locus_tag="PLK75_06155"
     CDS             complement(132..1046)
                     /locus_tag="PLK75_06155"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOY38867.1"
                     /translation="MVDLSINFNGLMLRNPVLTASGTFGYGEEFADFIDLNLLGGFIV
                     KGTTLLPREGNNYPRMAETPMGMLNAVGLQNKGVEYFCSVIYPRIKHFNTNILVNLSG
                     SQISDYVQGAEILNELEAIPAIELNISCPNVKEGGMAFGTSCASAAHVVKEVRSVYRK
                     HLMVKLSPNVTDIAEIAKSVEAEGADSVSLINTLLGMAVDVNTRKPKLSTITGGLSGP
                     AVKPVAVRMVWQVYKAVKIPVCGLGGIMNASDAIEFILAGASCIQVGTANFIDPAVTM
                     KIIDGISEYCYKNNVNKISELTGALAMN"
     gene            complement(1040..1807)
                     /locus_tag="PLK75_06160"
     CDS             complement(1040..1807)
                     /locus_tag="PLK75_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOY38868.1"
                     /translation="MRKYIHDLIVYKNIALNDEYFILELTHPEPLPDIFPAQFAEVSV
                     PDKHGVFLRRPISIHDVDYDSNIVKLLIQIVGDGTKKLSQISTGSTLNLVYPLGNSFS
                     MPSKPNLLLVGGGCGMAPLLYTARYFTSFGFSIKVLIGARTKENILLRDEYRKHAELL
                     TCTEDGTHGTKGYVTDHPVLSEPEGIDVIYACGPTPMMKAIAKYAEKNNIECEVSLEN
                     VMACGIGACLCCVQKTIHGNKTVCNDGPVFNIKEIVW"
     gene            complement(1837..2238)
                     /locus_tag="PLK75_06165"
     CDS             complement(1837..2238)
                     /locus_tag="PLK75_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY38869.1"
                     /translation="MKDRIAKILSDEGITGAKFAEEIGVQASSISHILSGRNNPGTDF
                     LIKILERYRGISAEWLLLGKGEMHKQSNHDPKRSISVNSEPDLFNPPSPTSQMLNSNA
                     NDKAKETNLVLSVEKIIVFYTNNTFKTYFPE"
     gene            complement(2847..3902)
                     /locus_tag="PLK75_06170"
     CDS             complement(2847..3902)
                     /locus_tag="PLK75_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY38870.1"
                     /translation="MLPLLIFIAADEIWGTQVGLYVAVATGITELIIIYIKQHKLEKF
                     VLLDTLLIVALGIISLLTENAVFFKTKPAIIGVILCVIVGISVFSGKNLMLLMSRRYL
                     KNFSIDKEAEKRLNFTLTILFWILVMHTLLVLYAAFYMSERAWAFISGPLLFIVFGAF
                     IFFEFLRVRFHKPLAVTPFSEWVPLVDENGRLTGKATREAVHNGSKLLHPVVHLHIIN
                     GNRMILLQKRSISKSIQPGKWDTAVGGHVDLGETIEAALLREASEELLIENLKPVFMI
                     RYIWESEIEKELVFSFVAFMNSIPDFEKSEIEEVRFWSQAEIKTELSNGLFTPNFEFE
                     FSILQKELPKIISKTQK"
     gene            4038..5852
                     /locus_tag="PLK75_06175"
     CDS             4038..5852
                     /locus_tag="PLK75_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HOY38871.1"
                     /translation="MKTILSFLTRHRQVLVFLFLITMVMCFPFAAEARMGGAGGGGSS
                     NGGGGGDGGIIYLILYLLINPDFFPWNIIILAIIVVLYFILRKKAKQRSILNSIPNTA
                     KMQAEGVKTISLPGLDVASFTEKVKTAFVQLQEAWAAKNMGKVRRYISDGMYQRLNIQ
                     FKMMDMLKQLNELAKIDVKGVYLDKAYQDGVNDVVHVAIHAVITDKFISEEFKQLASG
                     GREEFVEYWSFIRKTGAVEKDMYHTVNCPNCGGELPLDGGEISKCPYCSTITNSGAYD
                     WVLAEITQADDYKSGNALHDLSRNLGDRLEGLTQEYPDFSVQYIEDITSNGFLQIETA
                     RVLRKPELMRRFVSNELFEKLSTEIQNSSPFLYNRIFLSDVTLIGGLSDSNINSLTVS
                     VKMSYQRVSVSKGSLTIIDPAVVSSSRIVLLSRDAKAQQPKASLYAHQCPSCGGTLGD
                     TTDLTCPYCGATINSMMYEWIITDIMDIADYTQFYRTQYDKFAAGIKPGKIDAMFKIR
                     DYAFNNVLIIIAADGKFTQEEIDFANLIAKKWGYDTNKIQGMIDMAKNNQLVLRMPDD
                     KKDREKIYKLMEKAAHIDNELDPTEQALLEHVKKEYLS"
     gene            6058..6558
                     /locus_tag="PLK75_06180"
     CDS             6058..6558
                     /locus_tag="PLK75_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY38872.1"
                     /translation="MNIDNLNHRLIQAQSGLTYYALSLTNDEDSAKDLVQETFLKVLS
                     NTEKYTNDTNFNAWLFTILKNTFINTFNKLSTKKTIRDTSDDEYILRNTRYDAETPDS
                     VLGNSEIVHALESLEDDYKIPFTSFVNGYKYHEIAEMMNLPIGTVKSRIYFARKKLMD
                     VLTDFR"
     gene            6692..8233
                     /locus_tag="PLK75_06185"
     CDS             6692..8233
                     /locus_tag="PLK75_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38873.1"
                     /translation="MKYFLLIVLFIATCNNLFSQFGLKSGINSYSKEQYYTCIRKLEP
                     VANNTKTDPIKRSEAAFYVGLSYLKLSRPADAVGYFRLSISSGPGINEAYLFLGESLQ
                     MIEQFNEAEKNYQKYISLAPDDTIGRNKYNSLKYCKERMQQRSEYYVQIMPSFNTAEM
                     DYCPFFDAKDYSTVYFTSSRYLESKVPTNKESGESYSNIYRTTQLKDGFWSLAEKIPG
                     DVNTKYDEGTASLNRQSKNLYFTRCLHSTRSDKACRIYVATKSGNYWTKAEQVYIPEL
                     PEKVSIGHPCISYDELTLYFTADSLPGSYGGKDIYKVSRAKKSDLFGAPQNLGPAINT
                     RCDESYPYVRSDNIFYFASNGHPGMGGYDIFKAVPLGGVAFEIENMGYPINSPHDDFG
                     IVFMDDEEQGYLSSERIGGMGKSDIYLFYKTEITFDVSGTVWDKQANKPLSNVKISLM
                     NTEYKVLATFETNQVGKYMIKLDKKSDYYLYFYHSDYKTEKIEISTNDLNETKHFTRE
                     IFLVK"
     gene            8267..>8833
                     /locus_tag="PLK75_06190"
     CDS             8267..>8833
                     /locus_tag="PLK75_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38874.1"
                     /translation="MKRIITILILTFAGSLLFAQQSLIEKGDEAFDSYMFEIAADYYQ
                     KALPAIKTKDEKARVCFRLGISYFEIGKFTQAEESFTEAFTIITYENADNPELNKGEV
                     LLKYADALRMSGKYEKAINIYELYLKKEPNDYRALNGKESCINAPKWINRPTRYKVTN
                     VANFNSAKLDFSPVWSSKDYRSIYFTTSR"
     assembly_gap    8834..8843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8844..10370
                     /locus_tag="PLK75_06195"
     CDS             <8844..10370
                     /locus_tag="PLK75_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY38875.1"
                     /translation="DGTLGNRDNYKSGQKFTDLFEAAQDRKGTWSEPTPLVGEINSEF
                     DEGAASVGPRGTEIIFTRCEAGKNIDIPCKLYFASKRGNVWSTPVEINIPGFEGFEVG
                     YPAISKTGNHVVFASNKSGGYGGMDLYKADFDIKTGQISNVANLGYNINTTGDEVFPY
                     LKEDNKLYFASDGRGGMGGLDIFVATPYEGGLFKDAQNLRYPINSSYDDFGIVFKGNN
                     EEGYFSSNRPGGKGGDDIYSFILPPLIVKVQGFVKDTTDMSKIVRLKDASVTLLDDQS
                     IVKEMKSDATGAFSFELVPGKNYTLKAMVNENYFANSVSFTTKGVEYDTVIFIDLNLA
                     KIPLIIELPNIEYELAKADLRPESTVSLDNLVKTLNDNPHITIELRAHTDYRGGDDYN
                     MDLSYRRAKSCVDYLVLNGIDSLRLTSKGFGETMPKVIDEKTAAQFPFLKAGDVLTPE
                     FIDKLPGKEKQEICHQLNRRTEFSVLRKDYNLIDEHSINEDNKDGNLNKPPQNKSEDF
                     "
     gene            10468..11640
                     /locus_tag="PLK75_06200"
     CDS             10468..11640
                     /locus_tag="PLK75_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOY38876.1"
                     /translation="MENQERYSQRGVSAGKEDVHNAIKNLDKGVFPKAFCKIIPDYLG
                     GDDAFCNIMHADGAGTKSSLAYLYWRETGDMSVWKGIAQDSIVMNIDDVICVGATHVP
                     ILLSSTIGRNKKLIPGEVISALIEGTEELCDEYRRLGIDIRLTGGETADIGDLVRTIV
                     VDSTVVARMPVRDIISNEGIVPGDVVIGLSSSGKAVYENVYNGGMGSNGLTSARHDVF
                     ANYLADKYPESYDNAIEKNLVYCGVHRLTDVIENTGLDAGKLVLSPTRTYAPVIKEIL
                     QNMRSEIHGLIHCSGGAQTKILNFIENLSVVKNNMFSIPPLFRMIQQSAGTDWREMYR
                     VFNMGHRFEIYTSENIADEIISISKAFEIEAQIVGHCERSNTKSVIIETENGIFTY"
     gene            11654..12742
                     /gene="prfA"
                     /locus_tag="PLK75_06205"
     CDS             11654..12742
                     /gene="prfA"
                     /locus_tag="PLK75_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOY38877.1"
                     /translation="MAENLMIEKLEGVKKRYEEVKKLLADPSVMSDLEKYIKLNKEYK
                     DLTPIIAAFDEYKNILSNIDDAKKILSVEKDEEMREMAKEELDGLLWKVEPMEEKIRL
                     MLIPKDPEDDKNAILEIRAGTGGDEASIFAGDLFRMYSKFCEIKGWRIEIARVTEGTQ
                     GGFKEIVASVNGDGVYGVLKYESGVHRVQRVPQTETQGRVHTSAASVAVLPEAEEVDI
                     QLNPADIKRDEFCSSGPGGQSVNTTYSAVRLTHIPTGVVVICQDEKSKMKNLEKAMKE
                     LRTRLYNLEYQKYLDSIASKRKTMVSTGDRSAKIRTYNYPQGRVTDHRIGLTLYNLPA
                     IMDGDIQGMIDQLNMAENAERLKESGEN"
     gene            12760..13587
                     /gene="pyrF"
                     /locus_tag="PLK75_06210"
     CDS             12760..13587
                     /gene="pyrF"
                     /locus_tag="PLK75_06210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOY38878.1"
                     /translation="MNFEQIFEQILKKRSFLCVGLDSDIKKIPQHLLQEKEPVFEFNK
                     AIIDATAPFSVAFKPNLAFYESIGVEGWICLDKTIAYIREHYPEIFIIADAKRGDIGN
                     TSGLYARAFFENLRVDAVTVAPYMGEDSVSPFLSYHNKWVILLALTSNKGADDFQYLN
                     DKGSTLYEHVLTVSQQWGNKNNMMYVVGATRAQMLQGIRKIVPEHFLLVPGVGAQGGS
                     LKEVAEYGLNKSCGLLVNSSRNIIFANNTRDFAKAAAIEAAAMQSEMSSLLKSVMNL"
     gene            complement(13599..15221)
                     /locus_tag="PLK75_06215"
     CDS             complement(13599..15221)
                     /locus_tag="PLK75_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOY38879.1"
                     /translation="MNKILCVLIGIAFTIGSCQNNHAKFTEINIIPQPEKADLSGSSY
                     FVNKKTSISFDQNDANLLRAALRLQEILEKPLGVKVGMNVSEKKHNANSIKLSIDGNM
                     ADEAYVLSVCSDYVEIIGGTARGVYWGIQTLRQMMPENVEKDSVVDNFEIPCAVIYDA
                     PVFSYRGMHLDVCRHFFRVAEVKNYIDLLAMHKFNTFHWHLTDDQGWRIEIKKYPELT
                     QVGAWRDETVIGRNTGKYDSIRYGGYFTQDEVKEVVKYAEDRFITVIPEIEMPGHALA
                     AITSYPELGCTGGPYKVATLWGVFDDVFCAGNDKTFEFFQGVIDEVCEMFPNSPYIHI
                     GGDECPKGAWEKCPKCQARIKSEQLHDEHALQSYFITRMEKYINSKGKRIIGWDEILE
                     GGLAPDATVMSWRGEQGGIESARLGHDVIMTPNPICYFDHYQSKDTEKEPLAFGGFSD
                     CAKIYAWNPVPDSLTKAQSVHILGAQANLWTEYITNFSHAQYMVLPRMAALSEVLWCK
                     QKPGYNDFVRRLQFLFKRYDAAGYNYAKHLKQ"
     gene            15234..17789
                     /locus_tag="PLK75_06220"
     CDS             15234..17789
                     /locus_tag="PLK75_06220"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38880.1"
                     /translation="MNRFAKIIFYTILCTIAVLNTSCKRRPVYTSEKVLFSGEWQFLF
                     NEKYYPASIPGNIHSDLFANKLIDDPFFGDNEKNLQWIGQQSWHYTTNFTVTRDQLAQ
                     HNELVFEGLDTRSNILVNGIKIAETDNMFRKWVFNVENMIHEGSNTLEVVFLPVNQYN
                     DSMASALKYKLPDNRVFTRKAPYHSGWDWGPKYETCGIWKPVYLRSWKSFRIENFSIL
                     TLNANNNEAVIQANIIVESDTDQQAYLRLYSENNQQLLINKKVSLRKGRNELKTEFSI
                     EKPDLWQCNGRGKANMAQFRTELQSGRLTDTACCISGIRTAKLISETDSIGQNFYFEL
                     NGKPVFAKGANWIPMESFPGSVSRDKYRKLLTMAHDARFNMLRIWGGGYYEDDAFYEI
                     CDSLGIMVWQDFIFACAVYPGDENFQKNVEAEANSQVQRLRHHPCLVLWCGNNEVSNG
                     WNDWGWQQSLGYNSTDSASIWHSNRYLFEKLLPEIVEQNDGLRSYWPSSPMFGWGHPE
                     NNTHGDAHYWGVWWGKEDFEMYYEKTGRFMSEFGFQAMPSVECLKNFADSSDLNIDNP
                     LVANHQKHPYGFENIERGMLKYLYKPTSFEEYVYMSQVLQATAMQTAFDAHFSAAPHC
                     MGSLFWQYNDCWPSISWSAVDYCLNPKAVYYTAKRMFGEFSLCYRPKENFHVFYIFSD
                     HQDILDARAEIEICDFSGTHIAQATVRVTIIGGEAAAIISTQNSIFSTLKTKDNYLRI
                     KVYNNEQLLCSRAFFFERDNLLKLPESNIKISQNQSGDSIILQIESDKLSRWVALKAD
                     NLVFSDNYFDLMPGEKRLVTALPTTGELKQNHVIKVHSLNESMRFHRKHKNGN"
     gene            17903..21022
                     /locus_tag="PLK75_06225"
     CDS             17903..21022
                     /locus_tag="PLK75_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HOY38881.1"
                     /translation="MIIKGFDAAIFDLDGVITDTASLHSEAWKNTFDRVLALYSQDST
                     KNTKPFSIQTDYLNYVDGKPRYEGVRSFLESRNIFLEQGNSSDIEGMTTYCAIGNLKN
                     KEFNALLKSKGVQVFSSTVSFIENLIAHDIKIAVASSSKNCNPILKKQGLSKFFQASV
                     DGVYSEKYGLQGKPCPDIFITACEKLGAEPSRCIVVEDAVSGVEAAKNGNFGLVVGIA
                     RHNNHRELKSSGADIVVSDLEELGIETLNTWFETGLNEDNWCIKFNDFVPKKQKHRES
                     LLTVGNGYFASRGSMCEAIAGKHHYPATYMSGVYNTLPSVISGKTIYNEDFVNCPDWI
                     FTTFRIDDEQWIQPEDVKIIDIERRLDFRTGFLSGWMLIEDKQGRQTMLETIRFASMD
                     NCHIAALEHSVTPLNYEARISICTSLSASHINDGVERYRELNQKHLANVHTGHKGAQH
                     WLTCSTSNSATEISLIAHVSVPNHNCNLDYINGEDKITAVVSDDIKPNQDFVIQKTVA
                     LVNSQTHQKTLEYAKKELSKDSDFEVLALRSRKAWHKIWNITDIQIEGDRKSQKLVRL
                     NTYHLLVTASEHTSKIDVGIPARGLHGEAYRGHIFWDEVFILPFYNVHFPEVTRSVLQ
                     YRYNRLDAARRNTKENGYRGAMFPWQSAGNGNEQTQIIHFNPVSGQWDPDYSSLQRHV
                     SLAIALNIVRYYENTGDKAFMCTSGMEMLYEICRFWASATELGTDKKFHVYNIMGPDE
                     FHEKYPDNPEGGVTDNAYTNIMCVWLWNKTLKLTSSLGDKLKDVFERIGLEFFELDEW
                     QNISTKLNIPVIDNKILEQFAGFNQLEELNWQSYTEKYGNIGRLDRILKAEGKSPDSF
                     KLTKQADTLMAFYLLNQNELSEIAEDHSLDFSREAIQHSFEYYYSRCTHGSTLSSVVH
                     SQLASELGMQSLAWQLFKTSLESDYTDIQGGTTGEGIHTGAMSGSVLLLLNSFAGIKL
                     KGNTLSINPALPEHWHSIKCKISFRHTDIELIIFKDCLNIKASKDIGIRIQDVPLVIE
                     KDDWWNIDL"
     gene            21022..>21764
                     /locus_tag="PLK75_06230"
     CDS             21022..>21764
                     /locus_tag="PLK75_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY38882.1"
                     /translation="MDTMLSADNKIVHGLWIGNALSSTELLTIQSFTDNGHEFHLWAY
                     DSLSSVLPQGCILHNANDIIPRENIFKYKYSNQFGHGKGSYAGFSDIFRYKLLYLHGS
                     WWSDMDVTCLKPLDFEDEYVFRTHQSLPLVGNVMKCPKGSKLMQLCFEQASSLVSSEN
                     TDWHLPIQILCDNVFLLGLEKYIKDISNPDSWNLIRKLLIRGFDPPAGWYVIHWVNEE
                     WRRNNIPKNKALQSSFFYKLTKKYNVSIP"
BASE COUNT         6848 a         4319 c         4382 g         6205 t
ORIGIN      
        1 ctcaacatcc gtaagacctt ctggtattga atcattgaca gtttgtttgc ttttattact
       61 tccgcacgaa ataagcagaa taataaataa tgctggcaat aacgataatt tcattttcat
      121 tgtattgttg tttaattcat tgctaaagct cctgtaagtt ccgatatttt gtttacattg
      181 tttttataac aatactccga aataccatca ataatcttca tggtaacagc ggggtcaata
      241 aaattggcag taccaacctg aatacaagag gctcctgcca gaatgaattc gatagcatct
      301 gaagcattca ttattccgcc caatccgcac acgggaatct taaccgcttt atatacctgc
      361 cataccatcc ttacagctac aggcttcaca gccggaccag aaagccctcc tgtaatcgtg
      421 ctgagtttcg gcttccgagt attcacatca acagccattc ccaaaagtgt gttaattagc
      481 gaaacactat cggcaccttc ggcttcaaca ctttttgcaa tttcagctat atctgtaaca
      541 ttgggcgata atttcaccat taaatgcttt cggtaaacag accttacttc tttaacgaca
      601 tgtgcagcag aagcacagct ggtgccaaat gccataccac cctctttaac attcgggcaa
      661 ctaatattca attcaattgc tggaatagct tcaagctcat tgagaatttc agcgccctgc
      721 acataatcgc ttatctgcga tccgctcaaa ttgacaagaa tgttggtgtt gaaatgtttt
      781 atacggggat aaattactga acaaaagtac tccacacctt tattttgcag accaacagca
      841 ttgagcattc ccatcggggt ttcggccatt ctgggataat tgttcccttc gcgcggaagc
      901 aaggttgttc cttttacaat aaatcctccc agtaggttca ggtcgatgaa atcagcaaac
      961 tcttcaccat agccgaatgt tcctgatgcc gtaagaaccg gatttcttaa catcagccca
     1021 ttgaaattaa ttgacagatc taccatacaa tttcttttat gttaaagaca ggaccatcgt
     1081 tacaaaccgt tttattacca tgaatggttt tttgtacgca gcacaaacat gcacctatcc
     1141 cgcaggccat tacattctcc agcgaaactt cacactctat attgttcttt tcagcgtatt
     1201 tggctattgc tttcatcatg ggcgtagggc cgcaggcgta aatgacatct attccttctg
     1261 gttcggataa tacaggatga tcggtaacat atcctttggt accatgagta ccatcctccg
     1321 tgcatgttag caattctgca tgcttacggt actcatccct gagcagaatg ttttccttgg
     1381 ttcttgcacc aatcagcacc tttatagaaa acccaaaaga agtgaaatac cgcgctgtat
     1441 acaataaagg cgccatacca cagccaccac ctacaagcaa aagattgggc ttggagggca
     1501 tgctgaaact attccctaat gggtaaacaa gattaagcgt actgcctgtg ctaatctgac
     1561 ttaacttctt tgtaccgtct ccaacaattt gaatcagtaa cttaacaata ttgctgtcat
     1621 aatcaacgtc atgaatactt attggccttc gcaggaatac accatgctta tcaggcaccg
     1681 atacttcggc aaactgagca ggaaatatat ccggtaaagg ttcgggatgt gttaactcca
     1741 gaataaaata ctcatcgttg agcgcaatgt ttttgtaaac tattaaatca tgaatgtact
     1801 ttctcataga ttccagttta cgcaaatata cattatttac tccgggaaat aggttttaaa
     1861 tgtattgttg gtgtaaaata ctataatttt ttcaacagag agcaccaaat tcgtttcttt
     1921 tgctttgtca tttgcatttg aattgagcat ttgcgaagta ggcgaaggag gattgaataa
     1981 atcgggctca gaatttacag aaatacttct tttagggtcg tggttacttt gcttatgcat
     2041 ttcgcctttc ccgagaagca accattcagc acttatccca cggtatcgtt caagaatttt
     2101 gatcaggaaa tcggtacccg gattatttcg ccctgaaagt atatgagaaa tgctcgatgc
     2161 ctgaactccg atttcctcgg cgaactttgc tccggttata ccttcatctg atagaatttt
     2221 tgcaattcgg tctttcattt ttaaactatt ttctaacatg acaaatgtaa acattattta
     2281 attcacatat gtaattttta tcatatttac atttgtaatt atgacacaag ttgtaatcta
     2341 tagtattatt gtaaatatgc attgctattc aagtcattaa taaaatatag aaaaaagaac
     2401 tgctaatcga agttaaaatt catataaagt ttatatatat ctggtttaca attatttata
     2461 aatataattt atgcttattt tattatttat ttcatcaaaa tacacaataa tacacaaatt
     2521 aataaattat tactttagtt attaaattta acagattgat ttattgtaaa ttaaataata
     2581 aatatttact tattttatga ctacgtaaag actaattaat tgctttacaa tagtgaattt
     2641 aaatgtattg aaattattat tgctcttggg ttacatttgt taatatttac aattatcaca
     2701 gatattatat ttaatattgt aaatatcatg caaattttgg aaattccaaa tttcgcaaat
     2761 tcgacataat ctattcaaaa aggtgttttc cattcttttg aaaaaagagt cgaaatttgg
     2821 ggcaaaaaac tgccttttac agggtgttat ttttgtgttt tagaaattat ttttgggagc
     2881 tccttttgaa gaatcgaaaa ctcaaactca aaatttggcg taaataatcc attactcaat
     2941 tctgttttta tttcagcctg actccagaac ctcacctcct ctatctctga tttttcaaaa
     3001 tcaggtattg aattcatgaa agcaacaaaa ctgaacacaa gttctttttc gatttcgctt
     3061 tcccaaatat agcgaatcat aaaaaccggt tttaaattct ctattaataa ctcttcactg
     3121 gcttctctga gcaacgcggc ttcaatagtt tctcccaaat cgacatgacc tccaacagcc
     3181 gtatcccatt tcccgggctg aatactcttc gaaatgctcc tcttctgaag caatatcatt
     3241 ctgtttccgt taattatatg caaatgaact actggatgca aaagcttgct tccattatga
     3301 actgcttcgc gggtagcttt tccggtcaat cgcccgtttt catcgactag cgggacccat
     3361 tcactaaagg gtgtaacagc caacggttta tgaaaccgaa ctcttaggaa ttcaaaaaat
     3421 ataaaagcac caaaaactat gaaaagcaaa ggaccgctta taaaagccca tgccctttca
     3481 ctcatataaa atgctgcata taaaaccaat aatgtatgca taactaaaat ccaaaacaga
     3541 attgttaatg taaaattcag gcgtttttcg gcctccttat caattgaaaa atttttaagg
     3601 tatctacgcg acattaacag catcagattc ttacctgaaa atactgatat acctacaatt
     3661 acgcataaaa ttacaccgat aatggcaggt ttggttttaa agaaaactgc attttcggtt
     3721 agtaacgaaa tgattccaag ggcaacgatg agcaacgtat cgagaaggac aaacttctcg
     3781 agtttatgtt gtttgatata tattattata agttcggtaa tgcctgttgc aacagctaca
     3841 tacaaaccta cctgcgtacc ccatatttca tcagccgcaa tgaaaataag cagtggaagc
     3901 atgcccggta aaagattttt aagtaaagat gcctgtttca tagatacaca accataggtg
     3961 caaaaatatt ctttatcgtt gttattttaa caatttatta tgtactttta caattgattg
     4021 acacaaaaac aataaccatg aaaactattc tttcttttct cacacgccac aggcaggtat
     4081 tagttttttt atttttaatt acaatggtca tgtgtttccc ttttgcagct gaagcccgaa
     4141 tgggcggtgc cggaggtgga ggcagcagca atggcggagg cggaggtgat ggtggaatta
     4201 tatatttaat attatacttg ttaattaacc cggatttttt tccctggaat attataatat
     4261 tagcaataat agttgtattg tattttatcc tacgaaaaaa agccaaacag cgttctattc
     4321 ttaattcaat tcccaataca gcaaaaatgc aggctgaggg cgtaaaaacc atttctttac
     4381 ccggtttgga tgttgcctca ttcaccgaaa aggttaaaac tgcttttgtt caactgcagg
     4441 aagcctgggc agctaaaaat atgggtaaag tcaggcgcta cattagcgat gggatgtatc
     4501 agcgattgaa cattcagttt aaaatgatgg atatgctcaa gcagctaaac gaactcgcta
     4561 aaatcgatgt taagggtgtg tacctcgata aagcctatca ggatggggtg aatgacgtag
     4621 ttcacgttgc cattcatgca gttattaccg ataaatttat ctcggaggag ttcaaacagc
     4681 ttgcttcagg tgggcgtgaa gaatttgtgg aatactggtc atttataaga aaaacgggag
     4741 ctgttgaaaa agatatgtac cacaccgtaa attgccccaa ttgcggtgga gagcttcccc
     4801 ttgatggtgg agagataagc aagtgtccgt actgttcgac cattaccaat agcggtgctt
     4861 acgactgggt tcttgcagaa atcacccaag ccgacgatta caaatcgggg aatgcgctac
     4921 atgaccttag ccgcaatctt ggcgacaggc tcgaagggct cactcaggaa taccctgatt
     4981 tttcagttca atatatcgaa gatattacca gtaatgggtt tcttcagatt gaaactgcaa
     5041 gggtgctgcg aaaaccggag ctgatgagaa ggtttgtcag caacgaattg ttcgaaaaac
     5101 tatcaacaga aatacaaaat tcatcaccat tcctgtataa cagaattttc ttaagcgatg
     5161 taaccttaat tggaggactt tctgatagca acatcaacag ccttacagtt tctgtaaaaa
     5221 tgtcgtacca acgggtatct gtttcgaaag gttctctcac cattattgat ccggcggttg
     5281 tttcttcttc acgaattgtg ttgctcagca gagatgccaa agctcaacag cccaaagcat
     5341 cgttgtatgc acaccagtgt ccctcatgcg ggggaacatt gggcgatact actgacctca
     5401 cctgccctta ttgtggtgca accattaact caatgatgta tgagtggatt attaccgata
     5461 tcatggatat tgccgattat acccaattct acagaactca gtacgacaaa tttgctgccg
     5521 gtattaaacc gggaaaaatc gatgcaatgt tcaaaattcg cgattatgct tttaataacg
     5581 tactgataat aattgctgca gacggaaaat ttacgcagga agaaattgat tttgccaatc
     5641 tgattgcaaa aaaatggggt tatgatacaa ataaaatcca gggcatgatc gatatggcta
     5701 aaaacaacca gctggtatta cgtatgcctg acgataaaaa agaccgtgag aaaatttata
     5761 aactcatgga aaaggctgct catatcgaca atgagcttga cccaaccgaa caggctttgc
     5821 ttgaacatgt taaaaaagaa tatctttcgt aagcagatct ggctaatatt atttggcgta
     5881 ttaaaagcgg acagtataat tttaggtaat tttacgaatt ttaatgaatt ttattatacc
     5941 ctatctgttt gattatcaat aattaaataa tttttaacat tttttttatg aaaatatttg
     6001 gaatttaaaa ttaattaggt atttttgtac ctaaataact atttaataca acacatcatg
     6061 aatatcgaca atttaaacca cagattaata caagctcaga gcggccttac ctactatgca
     6121 ctaagcctta caaatgacga agacagtgcc aaggaccttg ttcaggaaac tttcctgaag
     6181 gttctgtcaa ataccgaaaa atatacgaac gatacaaact tcaacgcatg gttgtttaca
     6241 atcttgaaaa atacgtttat aaacacattc aataagctat caacgaaaaa aacgattcgt
     6301 gacacaagcg acgacgagta tattttacgc aacactcgtt acgatgccga gactcctgat
     6361 tccgtgcttg gtaatagcga aattgtacat gctttggaat cgctcgaaga tgattacaaa
     6421 atacccttca ccagttttgt aaatggttat aaatatcacg aaattgcgga aatgatgaat
     6481 ctgcctattg gcacggttaa gagtcgcatt tattttgcaa ggaaaaaact gatggatgtt
     6541 ttgactgact tcaggtaatt cgatagaaaa taaattaaag cagccttatt attacaccaa
     6601 attttagggc tgtttttttt tctatttcat cccggttatt gttgcatttt taaaaattat
     6661 ctatatttat gcacattaat cataaaaatt gatgaaatac tttcttttaa ttgttttatt
     6721 tattgcaacg tgcaacaatt tattcagtca gtttgggctt aaaagcggaa taaattcata
     6781 ctccaaagag caatactata cctgcatccg gaagttagag cccgtggcaa ataacacaaa
     6841 aaccgaccct atcaaaagat cggaagctgc attttatgtt ggactttctt atttgaagct
     6901 ctcgagacct gcagatgcag ttgggtattt caggttatct atttcttcag ggccaggcat
     6961 taacgaagca tatctttttc ttggagaatc gctgcaaatg attgagcagt tcaatgaagc
     7021 agaaaaaaat taccagaaat atatttcgct tgcacccgac gatactattg gaagaaataa
     7081 atataatagt ctgaaatatt gcaaagaacg gatgcagcag aggtcagaat actatgttca
     7141 gataatgccg agttttaata ctgcagaaat ggattattgc ccgtttttcg atgctaaaga
     7201 ttacagcaca gtatatttca ccagttcacg ttatctcgaa agtaaagtgc caacaaacaa
     7261 ggaatccggt gaatcgtatt caaacattta cagaaccaca caattgaaag atgggttctg
     7321 gtcgttggcc gaaaaaattc cgggagatgt taatacgaaa tacgatgaag gtactgcttc
     7381 acttaacagg caatctaaaa atctctactt cacccgctgt ttacacagca cacgaagcga
     7441 caaagcatgc agaatatatg tggccacgaa aagcggtaat tactggacta aagctgagca
     7501 agtatatatt cctgagcttc ccgaaaaggt ttccatcgga cacccctgta tttcgtatga
     7561 cgaattaacg ctatatttta ctgctgatag tttacctgga agttatggtg gtaaagacat
     7621 atataaggtt tcaagggcaa aaaaatcgga tttatttggt gctcctcaga accttggtcc
     7681 tgcgataaac acacgttgcg atgaaagcta tccttacgtc cgttccgaca acatatttta
     7741 ctttgcttca aacggtcacc cgggtatggg tggttacgac atttttaaag ccgttccact
     7801 tgggggagta gcattcgaaa tcgagaatat ggggtatcct ataaactctc cgcacgatga
     7861 ctttggcata gtttttatgg acgatgaaga acaaggttat ttgtcatcag aacgtattgg
     7921 tggtatggga aaatcggaca tatatctttt ttataaaacc gaaatcacct tcgatgttag
     7981 cggcacggtt tgggacaaac aggcaaataa acctctaagc aatgttaaaa taagcctgat
     8041 gaataccgaa tataaagtat tggctacttt cgagacaaat caggttggga aatacatgat
     8101 caagctcgat aaaaagtcgg attattattt gtatttctat cattctgatt ataaaactga
     8161 aaaaattgaa atctcaacca acgatttaaa cgaaaccaaa cactttacta gagaaatatt
     8221 tcttgtaaaa tgactttaag aatgtatatt tgaaaaattt ttttttatga agcgcattat
     8281 taccatactg attttaacat ttgccggttc actgctattt gcccagcaat cgttaatcga
     8341 aaaaggcgac gaagccttcg acagttatat gttcgaaatt gcagctgatt attatcagaa
     8401 agccctgccg gcaattaaaa caaaagatga aaaagccaga gtttgctttc gactaggaat
     8461 ctcctatttc gaaatcggca aattcactca ggctgaagaa agctttacgg aagcttttac
     8521 aattataacg tatgagaatg ccgataatcc cgaattaaac aaaggtgagg tattgctgaa
     8581 atatgccgac gcattgcgaa tgagcggaaa atacgaaaaa gccatcaaca tatacgagtt
     8641 atatttaaaa aaagagccta acgattaccg tgctttgaac ggtaaagaat cgtgtatcaa
     8701 tgctcccaaa tggattaacc gcccaacgcg ttacaaagta accaatgttg caaatttcaa
     8761 ttcggcaaaa cttgactttt caccggtatg gtcatctaaa gattatcgaa gcatttactt
     8821 cacaacatcg cgtnnnnnnn nnngatggta cattaggaaa cagagacaat tacaaatcgg
     8881 ggcagaaatt caccgacctt ttcgaagcag ctcaagatcg taaaggcacc tggagcgaac
     8941 cgacaccact tgttggagaa atcaattcgg agtttgacga aggagctgct tcggttggtc
     9001 ccagaggcac tgaaataata ttcacccgtt gtgaagccgg taaaaacatc gacattccct
     9061 gtaaactgta ttttgcatct aaaagaggaa atgtttggtc aactcctgtc gaaataaata
     9121 ttcccggatt tgaaggcttt gaagtgggat atcctgctat tagcaagacc ggaaatcatg
     9181 tggtttttgc gagtaacaaa tcgggtggct atggtggcat ggacttgtat aaagctgact
     9241 ttgacatcaa gacaggccag atatcgaatg tagctaatct gggatacaat atcaatacca
     9301 ccggtgatga agtattccca tatctgaaag aggacaataa attgtatttt gcttccgatg
     9361 gcagaggtgg tatgggcggt cttgacattt tcgttgcaac accttatgag gggggccttt
     9421 ttaaagatgc ccaaaatctc cggtatccga taaattcctc atacgatgat ttcggtatcg
     9481 ttttcaaagg caataacgaa gaagggtatt tctcctcaaa tcgcccgggc ggcaaaggtg
     9541 gcgacgacat ctacagtttt attctccctc cgctgattgt caaggtgcag ggatttgtta
     9601 aggacacaac agacatgtct aaaattgttc gtttgaagga tgcatcggta accctactcg
     9661 atgaccagag cattgttaag gaaatgaagt cagatgctac cggtgctttc tcatttgaac
     9721 ttgtgcctgg gaaaaactat accttaaaag ccatggttaa tgaaaattat tttgcaaatt
     9781 ccgtttcgtt tactaccaaa ggcgttgaat acgacactgt aatttttatc gacctgaatc
     9841 tggctaagat tccattgatc attgaacttc caaatattga atatgagctt gctaaagccg
     9901 atttacgccc tgaatccacc gtttcacttg acaaccttgt aaaaactctg aacgataacc
     9961 cgcatataac aatagaactc cgtgcacata ccgattacag gggtggtgat gattataaca
    10021 tggatctctc ttaccgtcgt gccaaatcgt gcgttgatta tctggttctt aatggtatcg
    10081 attcacttcg ccttacttcc aaaggttttg gagaaacaat gcccaaagta atcgatgaaa
    10141 aaaccgctgc acaattcccc tttctgaaag caggagatgt tcttactcct gagtttatcg
    10201 ataaactccc tggcaaggag aaacaggaaa tatgccatca gttgaacaga cgtactgaat
    10261 tcagtgtact tcgcaaagat tataatctta ttgatgaaca ttcaataaat gaagataata
    10321 aagacggaaa cctgaataag cctccccaaa acaagtcgga agacttctag ggcttttcaa
    10381 agcattatag tgcctgcgtt ttcttaactc aggcactatt tttattacca agttaattta
    10441 ttaattttgt gcgccaaatc aaatacaatg gaaaatcagg aacgctactc tcaacgcgga
    10501 gtgtcggcag gtaaggaaga tgttcacaat gcaataaaaa acctcgataa aggggttttc
    10561 ccaaaagcat tttgcaaaat tatccctgat taccttggtg gtgacgacgc tttctgcaat
    10621 atcatgcacg ctgacggagc aggaactaag tcatcgctgg catatttgta ttggcgcgaa
    10681 acgggcgaca tgagtgtttg gaaaggcatt gcgcaggact ctattgtaat gaatatcgat
    10741 gatgtgattt gtgttggagc tacccatgtg cctattctgt tatcatcaac cataggaagg
    10801 aataaaaaac taatacccgg tgaagtaatt tctgccctca tcgaaggcac tgaggaactc
    10861 tgcgatgaat accgacgact gggtatcgac atccgactta ccggcgggga aactgccgac
    10921 atcggtgatc tggtacgaac aatagtcgtt gattcaaccg tagtagcacg tatgccggta
    10981 agagatataa tttcgaatga gggaattgtt cccggagatg ttgtcattgg tttatcttcg
    11041 tcgggaaaag ctgtttacga aaacgtatat aacggaggca tgggcagcaa cggacttaca
    11101 tcggcgcggc acgatgtgtt tgctaactac cttgctgata agtaccccga aagctacgat
    11161 aacgctattg aaaaaaacct ggtgtattgc ggagtgcaca ggcttaccga tgttattgaa
    11221 aacacaggtc tcgacgctgg gaaactggta ctttccccaa caagaactta tgcccctgtt
    11281 ataaaagaga tactgcaaaa catgaggagt gaaatacacg gcctgattca ttgctcaggg
    11341 ggagcacaaa caaagatttt gaattttatt gaaaacctga gtgtagttaa aaataacatg
    11401 ttcagcatcc ctcctctttt cagaatgata cagcaatcgg ccggtaccga ctggcgcgaa
    11461 atgtaccgtg ttttcaatat ggggcatcgt ttcgagattt ataccagtga aaacattgct
    11521 gatgaaataa tttctatcag caaagccttt gaaattgaag cgcaaatagt tggacattgt
    11581 gagagaagca atacaaagag tgtaattatc gaaacagaaa acggaatttt tacctattaa
    11641 tttatattga tttatggcag agaacctgat gattgaaaag ctcgaaggag taaaaaaacg
    11701 ctatgaagaa gtaaaaaaac tgctggcaga cccttcggta atgtcggatt tggaaaaata
    11761 tataaagctg aataaggaat ataaagacct cacccccatt attgctgcct tcgatgaata
    11821 taaaaacatt ctatcgaata ttgacgatgc aaaaaaaata ctttctgtag aaaaagacga
    11881 agaaatgaga gaaatggcaa aagaagaact cgacggcttg ttatggaagg tggaaccgat
    11941 ggaggaaaaa atcaggctta tgctaatccc caaagaccca gaagacgata aaaatgccat
    12001 tcttgagatt cgtgccggca cagggggtga tgaagccagt atttttgctg gcgatttatt
    12061 tcggatgtac tcaaaattct gtgaaataaa gggatggagg attgagatag ctcgtgtaac
    12121 agaagggaca cagggtggct tcaaggaaat tgtcgcaagt gtgaacggag atggtgtgta
    12181 tggcgtttta aaatatgaat caggggtgca tcgtgtacag cgggttcccc aaaccgaaac
    12241 acaggggcgt gtacacactt cggcagcttc agttgcagtt ctccccgagg ctgaagaggt
    12301 cgatattcag ctcaaccctg ctgatattaa acgggatgaa ttctgttcat cagggccggg
    12361 agggcaaagt gtgaacacaa cgtattctgc agtaaggctt acgcacattc caaccggtgt
    12421 tgtggttatt tgtcaggatg aaaaatcaaa aatgaaaaac ctcgaaaagg cgatgaaaga
    12481 actacgcacc cgactgtata atctggaata ccaaaagtat ctcgactcta ttgcaagcaa
    12541 gcgcaaaaca atggtatcga caggtgacag atctgcaaaa atacgaacct acaattatcc
    12601 tcagggcagg gtgaccgatc atcgtatcgg gttgacactc tacaaccttc ctgctattat
    12661 ggacggagac attcagggaa tgatcgacca gctgaatatg gctgaaaatg cagagagact
    12721 gaaagaatcc ggtgaaaatt aacatcaaaa atacctctta tgaatttcga acaaattttc
    12781 gagcaaatac taaaaaaaag aagcttctta tgcgttggac tcgatagcga tataaaaaaa
    12841 ataccacaac atcttcttca ggagaaggaa cctgtgtttg aattcaataa agctattatc
    12901 gatgccacag cacctttttc cgttgctttt aaacccaatc ttgctttcta cgaaagtata
    12961 ggtgttgaag gttggatttg ccttgacaag acaattgctt atatcaggga acactacccc
    13021 gaaatattta ttattgctga tgccaagcgt ggtgacattg ggaatacctc cgggttgtat
    13081 gcccgcgcat ttttcgaaaa tctaagggta gacgcagtta ctgttgctcc ttatatgggc
    13141 gaagactccg tttcgccttt cctgagttac cataacaaat gggttatttt acttgcacta
    13201 acatcgaata aaggtgccga cgatttccag tacttaaacg ataaaggatc aacattgtat
    13261 gaacatgtat tgactgtttc tcagcaatgg ggaaataaaa acaatatgat gtatgttgtt
    13321 ggtgcaacac gcgcacagat gttgcaaggt atcaggaaga tcgttcccga gcatttccta
    13381 ctggtccctg gtgtcggagc tcagggcgga agcttgaagg aagttgctga atatggtctg
    13441 aacaaatcct gtggtttgtt ggtaaactca tcgcggaata tcatttttgc aaacaatacg
    13501 cgcgattttg caaaagctgc tgctatcgaa gctgctgcta tgcagtcaga aatgagcagc
    13561 ctattgaaat cggtgatgaa tttgtagact aaacttattt attgtttcag atgcttggca
    13621 taattgtagc cggcagcgtc gtaacgctta aataaaaact gcagccttct gacaaagtcg
    13681 ttatacccgg gtttctgttt acaccagaga acttcgctaa gagctgccat tcttggtagt
    13741 accatatatt gagcatggct gaaattagta atatattcag tccataaatt tgcctgagca
    13801 cctagaatgt gtactgattg tgctttggtc agactatcag gtacagggtt ccatgcatat
    13861 attttggcgc agtcagaaaa accaccgaac gctagtggtt ctttttcggt gtccttgctt
    13921 tgataatggt cgaaataaca aatcggattc ggagtcataa tcacatcatg gccaagccgg
    13981 gcagattcaa tacctccctg ctcccctcgc cagctcatca ctgtggcatc aggagccaaa
    14041 ccaccttcga gaatttcgtc ccagccaata attcgcttac cctttgagtt gatgtatttc
    14101 tccattcgtg tgataaaata actttgtaaa gcgtgttcat cgtgtagttg ttccgatttg
    14161 atacgtgctt gacatttcgg gcatttttcc catgctccct ttgggcactc atcccccccg
    14221 atgtggatat agggcgagtt tggaaacatt tcgcatactt catcaataac tccttgaaaa
    14281 aattcaaaag ttttatcgtt tccggcacaa aatacgtcat cgaaaacacc ccagagagta
    14341 gccactttat aaggacctcc ggtgcatccc agttccggat atgatgtaat tgcagctaaa
    14401 gcatgaccgg gcatttcgat ttcgggaata acggtaataa aacgatcttc ggcatacttt
    14461 acgacttctt tcacttcgtc ctgtgtaaaa taaccgccat aacgaatgct gtcatatttg
    14521 ccagtattac ggccaatgac ggtctcatcg cgccatgcac ctacttgagt aagttctgga
    14581 tactttttta tttcgatgcg ccagccttga tcatcggtca gatgccagtg aaaagtattg
    14641 aatttatgca tcgccagaag gtcgatataa ttcttaactt ctgcaacgcg gaaaaaatgc
    14701 ctgcacacat caagatgcat tcccctgtag ctgaaaactg gagcatcgta aatcacggca
    14761 caaggtattt cgaaattatc aacaactgaa tccttttcaa cattttcagg catcatctga
    14821 cgcagcgtct gtatacccca ataaacacca cgggcagtcc ccccaataat ttccacataa
    14881 tcgctacaga ccgataaaac atacgcttcg tcggccatat tgccatcaat agaaagttta
    14941 atgctgtttg cattatgttt cttctcagaa acattcattc cgacttttac cccgagaggt
    15001 ttctcaagta tttcctgtaa acgaagggct gctctcaaca ggtttgcatc attttggtcg
    15061 aaagaaatag atgttttttt attgacaaag tagcttgaac ccgataaatc tgctttttcg
    15121 ggttgcggaa ttatatttat ctccgtaaat ttagcatgat tattttgaca tgaacctatg
    15181 gtaaatgcaa taccaattag aacgcaaagt attttattca tacaattagt ttaatgaatc
    15241 gatttgctaa aataatcttt tacacaatac tatgcactat tgcagtttta aatacttcat
    15301 gcaagcgcag acctgtttac acttcagaaa aagtattgtt cagtggtgaa tggcagtttt
    15361 tatttaatga gaagtattac cctgcaagca ttccgggaaa tattcacagc gacttgttcg
    15421 ccaataagct cattgacgat ccattttttg gcgacaacga aaagaacctg caatggattg
    15481 ggcaacaatc gtggcattac actacgaatt ttactgttac cagagaccag ttagcacagc
    15541 ataacgagct tgtttttgaa ggtctagata cccgcagcaa cattcttgta aacgggataa
    15601 aaattgctga aactgataac atgttccgca agtgggtatt caatgttgag aacatgattc
    15661 atgaagggag caatacactt gaagtcgttt ttcttcccgt taaccaatac aacgacagta
    15721 tggcctcggc tcttaaatac aaactacctg acaaccgcgt tttcacccga aaagctcctt
    15781 accattccgg ctgggactgg ggcccgaaat acgaaacttg tgggatatgg aaaccggtgt
    15841 acttgcgttc atggaaatcg tttcgaatcg aaaattttag tatcctaact ttgaatgcga
    15901 acaataatga agccgtcatt caagccaaca tcatcgtaga aagcgatact gaccaacagg
    15961 cttatttgag attgtattca gaaaacaacc agcaactttt aatcaataaa aaagtatccc
    16021 tccgcaaagg cagaaacgaa ttgaaaacgg aattcagtat cgagaaaccg gatttatggc
    16081 aatgcaatgg acggggcaaa gcaaacatgg cacagtttcg cacagagcta cagtctggca
    16141 ggctaacaga cacagcctgc tgtataagtg gtatccggac tgcaaaactt ataagcgaga
    16201 ctgacagtat cggtcagaat ttttattttg aattgaatgg gaaacctgtg tttgccaaag
    16261 gagcaaattg gatacccatg gaaagctttc cgggtagtgt gagcagggac aaatacagaa
    16321 aattgcttac tatggcgcat gatgcaagat tcaatatgct ccgtatttgg ggcggcggct
    16381 attacgaaga cgatgcattt tatgaaattt gcgattcgct cggaatcatg gtttggcagg
    16441 attttatatt tgcctgtgcg gtctatccgg gcgacgaaaa ttttcagaag aatgtagaag
    16501 cagaggcaaa ctcccaggtt cagcgcttac ggcaccaccc ctgcctcgtg ctatggtgtg
    16561 gcaacaacga agtaagcaac ggatggaacg actgggggtg gcaacaatcg ttgggataca
    16621 acagcaccga ctcggcatca atttggcaca gcaaccgcta tctcttcgaa aaactcttgc
    16681 ccgagatcgt tgagcagaac gacggcttac gaagctattg gccaagttca cccatgttcg
    16741 gctgggggca tcccgaaaac aatacccatg gcgatgccca ttattggggt gtatggtggg
    16801 gcaaagaaga tttcgaaatg tattacgaaa aaaccgggcg ttttatgagt gaatttggat
    16861 tccaggctat gccctcggtt gaatgcctca aaaatttcgc agacagctcc gacctgaata
    16921 ttgacaaccc tttggttgcc aaccatcaga aacatccgta tggctttgag aacattgaaa
    16981 gaggtatgct gaaatacctg tataaaccaa catcattcga agagtatgtt tatatgagcc
    17041 aggtgctgca agccactgca atgcagacag ccttcgatgc gcatttttcg gcagcaccac
    17101 attgtatggg aagccttttt tggcagtata acgattgctg gccttcaata tcatggtcag
    17161 cagttgatta ttgcctgaac cccaaagcag tatattatac tgctaaacgc atgtttggcg
    17221 aattcagcct ttgctatcgc cccaaggaga atttccatgt gttttacata ttcagcgacc
    17281 accaggacat tctggatgca cgtgctgaga tagaaatttg tgatttttcg ggaacgcaca
    17341 tagcacaggc aacggtaaga gtaacaatca tcggaggcga agccgctgca ataatctcga
    17401 cacaaaactc gattttcagc acactgaaaa caaaagacaa ttatttgaga atcaaagttt
    17461 ataacaatga gcaattgttg tgtagccgtg cttttttctt cgagcgtgac aacctgctca
    17521 agcttccgga gtcaaacata aaaatcagcc agaaccaatc aggagacagc attattcttc
    17581 aaatagaatc ggataaactg tcccgttggg tggcactcaa agccgataat cttgtttttt
    17641 ccgacaatta cttcgacctg atgccggggg aaaagcgttt agtaaccgcc ttgccaacca
    17701 caggggaatt aaagcaaaac catgttatta aagtgcattc acttaatgaa agtatgagat
    17761 ttcatcggaa acacaagaat ggtaactaga actgcaattt taactgatta aatggatatc
    17821 aatcttaatg tttcctaaac attttgtttc atgttccaaa aaaactttat attagtcgct
    17881 taaatgaatt agcctaaaat aaatgataat taaaggattt gatgcggcaa tattcgatct
    17941 cgacggagta attaccgata ctgcctcgtt gcacagtgag gcatggaaaa acacattcga
    18001 cagagtgctt gcactgtata gccaagatag tacaaaaaat acaaaaccgt tttcgataca
    18061 aaccgattac ctgaattacg tggatggaaa gccgcgatat gaaggagtga ggtcattcct
    18121 ggaatcgcgc aatattttcc ttgagcaagg taatagttcc gacattgagg gtatgacaac
    18181 ctattgtgcc atcgggaacc ttaagaataa agaattcaat gcattgttga aatcgaaagg
    18241 agttcaagtt tttagctcta cagtttcttt tatcgaaaac ctgattgctc acgacattaa
    18301 aatcgcggtt gcttcatcaa gcaagaattg taacccaatt ttaaaaaaac aggggctcag
    18361 caaatttttt caagcttcgg ttgatggtgt ttattccgaa aagtatggac ttcaaggaaa
    18421 accttgccct gatattttta taactgcatg cgaaaaactg ggtgccgaac ccagcagatg
    18481 cattgtggtc gaagatgctg tgagtggcgt tgaagcagca aaaaacggaa atttcggatt
    18541 ggtagttggt attgcaaggc ataacaatca tcgggaactc aaatcgagtg gtgccgatat
    18601 tgttgtgagc gatctggaag aattgggaat tgaaacttta aatacttggt ttgaaactgg
    18661 cctcaatgaa gataactggt gcattaagtt caacgatttt gtacctaaaa agcagaagca
    18721 cagagagtcg ctactgacgg ttggaaacgg atattttgca tcgaggggct ctatgtgtga
    18781 agccattgca ggtaaacacc actaccctgc cacttacatg tccggtgtat ataacaccct
    18841 tccctcagtt atttctggga aaaccattta caacgaagat ttcgttaatt gcccggattg
    18901 gatttttact actttccgaa ttgatgatga gcagtggata cagcccgaag atgttaaaat
    18961 tattgatatt gaacggcgac tcgatttccg cacagggttt ctgagtggtt ggatgctgat
    19021 tgaagataaa cagggtagac aaaccatgct tgagaccata cgttttgcaa gtatggacaa
    19081 ctgccatatt gctgctctgg agcactctgt aacaccgctg aactatgagg cgcgaataag
    19141 tatttgcact tcgctcagtg cttcacacat taatgacggt gttgaaagat accgagaact
    19201 gaatcagaaa caccttgcca atgttcatac cggccataaa ggtgctcaac actggcttac
    19261 atgtagcacg agcaattcag caactgaaat ttccctgatt gcccatgtaa gtgtaccaaa
    19321 ccacaattgt aatttggatt atattaacgg tgaggataaa atcactgcag tagtgagcga
    19381 tgatattaaa ccaaatcagg actttgtaat tcagaaaacc gtagccctcg tcaatagtca
    19441 aacacatcag aaaacactgg aatatgccaa aaaggagctc tccaaagaca gtgatttcga
    19501 agtattggca ctaaggagcc gaaaagcatg gcataaaatc tggaatataa ccgatattca
    19561 aattgaaggt gatagaaagt cgcagaaact agttcgctta aacacctatc atcttttagt
    19621 tactgcttcg gagcatacat caaagattga tgttggaatt cctgcaagag gtttgcatgg
    19681 cgaagcttac aggggacata ttttctggga tgaagttttt attttgcctt tctataatgt
    19741 ccatttccct gaggttacac gctcggtgct tcagtaccgc tataatcggt tggatgctgc
    19801 acgaagaaat acaaaagaaa acggatatcg cggggctatg tttccctggc aaagcgcagg
    19861 caatggaaat gaacagacac agataattca ctttaaccct gtttccggac aatgggatcc
    19921 cgattatagc tcactgcagc gccacgtatc gcttgcaatt gcactcaata tagtaagata
    19981 ttacgaaaac accggcgaca aagcattcat gtgtacatcg ggtatggaaa tgctttacga
    20041 aatttgtcgt ttttgggcat cagcaacaga attgggtaca gataaaaaat tccatgtata
    20101 caatattatg ggtcccgatg aatttcatga gaaatacccc gacaatcctg aaggaggtgt
    20161 taccgataac gcatatacca atattatgtg cgtgtggtta tggaataaaa ccctgaaact
    20221 tacatcctcg cttggcgaca aactgaaaga tgttttcgaa agaatcgggc ttgaattctt
    20281 tgagcttgac gaatggcaaa atattagtac aaaacttaac attcctgtta ttgacaataa
    20341 aattcttgaa caattcgcag gattcaatca acttgaagaa cttaactggc aaagctacac
    20401 tgaaaaatac ggaaacattg gcaggctcga cagaatttta aaagcagaag ggaaatcgcc
    20461 cgactcattt aagctcacaa aacaagccga cacactaatg gccttctacc tgttgaatca
    20521 gaatgagctc agcgaaatag ccgaagacca ttcgcttgat ttcagccgtg aagctataca
    20581 acatagcttt gagtattatt acagcagatg tacgcatggc tccaccctca gcagtgttgt
    20641 tcattcgcaa cttgcatctg aattgggaat gcaatcgttg gcgtggcagc tttttaaaac
    20701 ttctctcgaa agcgactata ccgatataca gggcgggact accggtgaag ggatacacac
    20761 aggcgcaatg tcaggatcgg tattgcttct gctcaattca tttgcaggaa ttaaacttaa
    20821 agggaatact ctttcgataa acccagctct accggagcac tggcatagta ttaaatgtaa
    20881 aatcagtttc agacacaccg atattgagct cattattttc aaagactgcc tgaatattaa
    20941 agcttcgaaa gatataggta ttcgcattca ggatgttccg ttagtaattg agaaagacga
    21001 ctggtggaac attgatttat aatggacaca atgctatctg ctgataataa aatagtacac
    21061 ggactatgga taggtaatgc actatcgtca acagagctgc ttacaattca atcgtttacc
    21121 gataacgggc acgaatttca tttatgggct tacgacagtt tatcgtccgt tttgccccaa
    21181 ggctgtatac tccataatgc caatgacatt attcctcggg aaaacatttt caagtataaa
    21241 tactccaatc aatttgggca cggaaaggga agttacgcag ggttctccga cattttcaga
    21301 tataagctgt tgtatttaca tggcagctgg tggtccgaca tggatgttac atgcctgaaa
    21361 ccgcttgatt ttgaagatga atatgttttt agaacccacc agtcgcttcc gcttgtcggt
    21421 aacgtaatga agtgccctaa aggctcaaaa ttaatgcaat tatgttttga gcaggcaagc
    21481 agtttggtaa gctctgaaaa caccgactgg catctgccaa ttcaaattct ctgcgataac
    21541 gtttttttgc ttggcttaga aaaatatatc aaagatattt cgaaccctga cagctggaat
    21601 ctgatacgaa agctactgat tcggggcttt gatccacctg ccgggtggta cgtcattcac
    21661 tgggttaatg aagaatggcg caggaataat atccccaaaa acaaggcctt gcaaagttcg
    21721 ttcttttata aactgacaaa aaaatacaat gtcagcatcc ccat
//
LOCUS       DAOOQP010000043        21422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3001_length_21422_cov_6.415079, whole genome shotgun sequence.
ACCESSION   DAOOQP010000043 DAOOQP010000000
VERSION     DAOOQP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3001_length_21422_cov_6.415079"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLK75_06235"
     CDS             complement(<1..551)
                     /locus_tag="PLK75_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38883.1"
                     /translation="MRKLYTLIVIILLFGSLPKLTFSQAYTNLDFEVWTTSIIGDSPT
                     GWVTLNATKETSDVYSGNYALRLETKNSIMGLQPGLATIGVMQGLNVIPGEPYTGVPQ
                     TISGYIKYNLAEGDTALFYIRLTNNAEIIASADTMFAGVNTEWTFFSLQINWYTWGVP
                     DSIQMGITSGGNSSLIGYNIGTLT"
     gene            complement(654..726)
                     /locus_tag="PLK75_06240"
     tRNA            complement(654..726)
                     /locus_tag="PLK75_06240"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Lys,seq:ctt)
     gene            complement(765..838)
                     /locus_tag="PLK75_06245"
     tRNA            complement(765..838)
                     /locus_tag="PLK75_06245"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Ile,seq:cat)
     gene            1133..1759
                     /locus_tag="PLK75_06250"
     CDS             1133..1759
                     /locus_tag="PLK75_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOY38884.1"
                     /translation="MLIKNVVFDLGNVILNINPELSVKEFSKLGIPNFDKLYTLSLQT
                     ELFDDLETGRTNAGAFCEKVRIITNTNLHDSIIIDAWNALILDFPQENLELLKRARNK
                     YRTFILSNTNRIHYEYYTENLNKYHDIQNLESLVEKAFFSHEIGLRKPDKGIFNYMIN
                     ITGIDPSETLFIDDSRANCRTAEMLGFFVEEYREKNLPQLFRKLNLPF"
     gene            complement(1748..2161)
                     /gene="tsaE"
                     /locus_tag="PLK75_06255"
     CDS             complement(1748..2161)
                     /gene="tsaE"
                     /locus_tag="PLK75_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOY38885.1"
                     /translation="MPVIRIKSIAGVEKAAKQFLELTKTNRIVAFTGEMGAGKTTFIK
                     ALCKLLGVENMVNSPSFPIVNEYKTDAKVLIYHFDFYRIKNTVELLDIGIEEYFSGKA
                     KIFIEWPEKALELLPPETIMVKIIIDKDDSRTLEW"
     gene            complement(2163..3722)
                     /locus_tag="PLK75_06260"
     CDS             complement(2163..3722)
                     /locus_tag="PLK75_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOY38886.1"
                     /translation="MKKISILWVDDEIDLLRPHILFLQQKGYELETANNGYDAIEMVK
                     KKEYDIFFIDENMPGLNGIETLKVIKEIQPLVPVIMITKSEEENIMDDAIGSKINDYL
                     IKPVNPNQILLSIKKNIDHRRLIAQKTNSEYQMAFSKLGMEINDSLTHQDWINVYKKL
                     VYWELELSQSAESAMDEVLQMQKNEANSAFTKYIKKNYASWFGSSSGNKPILSPSLLK
                     EKVFPQVSSGKKTFLLVIDNLRFDQWKVIQPELNEFYKTETEDMYYSILPTSTQYARN
                     SLFSGLMPSEIDRLYPELWKNDEEEGGKNLYEEDMLKKLVRKYNISDNLFFEKINDIK
                     NLRKVNERVREIVDTSHFMVMIVNFVDYFSHAVTDQKMIKELVGNETAYRSITQSWFK
                     HSPLLELLRSLSELGVNLVITTDHGTIKVQNAIKVIGERNTTTNLRYKQGRNLNYNPK
                     EVFEVLKPESIYLPRTNLSSSYIFATGNDFFAYHNNFNHFVKYYRDSFQHGGVSLEEM
                     LIPVIHLSPRK"
     gene            complement(3776..6520)
                     /locus_tag="PLK75_06265"
     CDS             complement(3776..6520)
                     /locus_tag="PLK75_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38887.1"
                     /translation="MHLKFNIAQSKTSVYIVIGLLYLSLLGWSCSTEKNTFLSRTYHN
                     TTARYNIYFNAKESYKKGIKQAEASNTDSYISILPMFIISKPDAANSLVGEMDKTIKK
                     SSKAIKFHSITAKPKTKSGKLSAKEKEFYNKKEFCKWVDDSYLLMGMAYFAKHDFFEA
                     RQNFEYLIRQFPKELVAYDAKIWLARTFSESQKYKSAKEIFDLIEADKSMPKKYQGQF
                     AAAYADYYIKLGQYDDAIPKLKKAIKYAGKKSARIRYMYILAQLYEATGDAKRASEMY
                     AAVAKKNPVYEMEFNARINIARLYSGTGGKEVRKKLRRMLKDDKNIEYLDQIYYAIAE
                     IDYRDKKTDDAIKNYKLSSEKSMANNYQKALSCEKLGKIFFGRKDYINAQAYYDTCLA
                     NLPLDYKSYKEIQDLSNNLNDLVIELNTITLEDSLQKVAKMSEKDRNKLIDDIIADLI
                     EEENRQRELEQEQQMNSMLFDQRRDGYQNNVSAPSGGKWYFYNPSQLSFGRNEFVKKW
                     GNRKNEDHWRRKNKSVVSFDNETASTDSLTSDTLNTRKVSDNKKREYYLQDLPLTDSL
                     IASSNSRIMDAFYNAGRIYKEQFEDFDQSIFCFNELNRRFPNNIYLLTSYYNLYLLHK
                     IKGNASEMETYRNLIITKFPTSHYANLLQNPNYVNELKAKRESDIALYMNTYDAYVQG
                     KCQLVNTNTNKYFSGKTEKDLEARFRFISALCVGKTMDEMSFKKALVDFIGDYPNDEL
                     ARTAQDILSYFGSANIEELRKELAARPSIVNDDANLIFNTNTSDSLIAAEAVFKNNES
                     VPHYYIILANADQIDINRLSFEVRNFNIFTFSLKNFTVMNMLFDDKYQMVIVKTFDNK
                     RQSLNYMKIIKNNPDVFGRIESNDYKQFTISEENYQALKKVKNINLYLDFFETNYVNP
                     "
     gene            complement(6565..8400)
                     /gene="glmS"
                     /locus_tag="PLK75_06270"
     CDS             complement(6565..8400)
                     /gene="glmS"
                     /locus_tag="PLK75_06270"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOY38888.1"
                     /translation="MCGIVGYLGYRDAYTVLIRGLKRLEYRGYDSAGIALLNSKTRIY
                     KCKGKVSDLENLVADQDTSGNIGMGHTRWATHGEPTDVNAHPHLSMNKQFVVIHNGII
                     ENYNQLRNELVEEGYTFISETDTEVLANLIEYVYRKLEVPPEEAVRVALTKVIGAYGI
                     LVAFTQKANTIIAARKGSPLVIGKGQNEYFFASDATPIIEYTNSVIYLNDDDIAIVNK
                     SGLHLKSVSNITKKPEIQKIELDIEEIEKGGFDHYMLKEIFEQPRSITDTCRGRLNYY
                     DKSLKLGGLHAVGSKLIEADRVLIVACGTSWHAALVGEYLFEEFARIPVEVEYASEFR
                     YRNPIINKNDVVIAISQSGETADTLAAIRLAKDSGALVLGICNVVGSSIPRETHAGVY
                     THAGPEIGVASTKAFTAQVTVLIMMAMLVGKQNKSLTEKEYDDLIEELFEIPGKIESI
                     LAKSDEIKNIAELYKDATNALYLGRGYLFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDEKMPVIVLATKDKSYEKIVSNIQEVKARKGNVIAIVTEGDTVIKNMADHVIE
                     VPYSDCSLAPLLTVVPLQLLSYYIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(8408..9685)
                     /locus_tag="PLK75_06275"
     CDS             complement(8408..9685)
                     /locus_tag="PLK75_06275"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOY38889.1"
                     /translation="MTGIKMWSKVISQAFILSSIIFLMYSCEREPNEIGLNLQPGSDR
                     MGLLCDTLEVETYTVLDDSIASDERTLSPLGSYIDPVFGFCKADFFTHLRLSSSNVNF
                     SNVVETADSLVLRLFYTGFYGDTTTTQRIRVYEVLNQDIYPDSTYYSNFRFNPGDLSE
                     ICSVEITPHPSDSFVNIKMPDDLINRFISSSNVSSFVSNDTFLDFFKGLYITTDNVNA
                     GGAILYFNLISSNSEMTLYYNDSLSFDFLINASSARINMFEHDYSLASAEIQQTIDNL
                     NEPNTVSFLQSLGGLKVKLYIKGLEGFDTCRYAVNKAQLQITVNRANELGFAPPPKLT
                     LVAENEDGLNTFLTDYKINSSYFGGVLDESDYTYTFNIPFHLQELFSDQNLIDNGIFL
                     FPLDNRTDASRVTVYGGDSPVNSMKLIIMYSKF"
     gene            complement(9688..10500)
                     /locus_tag="PLK75_06280"
     CDS             complement(9688..10500)
                     /locus_tag="PLK75_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOY38890.1"
                     /translation="MSMSLKVLYVSQEITPYLPESEISVISRYLPQGVQEQGREIRTF
                     MPKYGCINERRNQLHEVIRLSGMNMVINDTDHPLIIKVASIQPARMQVYFIDNEDYFG
                     RKFALRDKKGSFFADNDERAIFYARGVIETVKKLCWAPDLVHCHGWFSALVPLYIRKT
                     YADDPLFSDLKIIYSVYNEDFKKELSADFSQKIMSEGISKEDVAVVEEANYVNLNKLA
                     IEYSNGVIMGSKNIHEDLHEYISNSGKPVLEYQGPDDYIYAYNKFYDLITRK"
     gene            10645..11490
                     /gene="panC"
                     /locus_tag="PLK75_06285"
     CDS             10645..11490
                     /gene="panC"
                     /locus_tag="PLK75_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOY38891.1"
                     /translation="MKVFTSHEDLNYCLSEYRSGGLTIGFVPTMGALHAGHISLIKKS
                     TGQNKVSVVSVFVNPRQFNDSNDFTNYPRNISADIKLIEAAGCEFVYTPDYQDLFPTE
                     DSLEFDFNGLDKVLEGAYRPGHFQGVVDVVHALFKAVRPNNAYFGLKDYQQLKIIQLL
                     ASQFHPKTNIIPCPIIREPDGLAMSSRNQRLQPDERKAAAMIPKILMESAAFTGNHSV
                     AELKANIVSRFNKAEGLKLEYAEICNSDNLEAIDSGNPAKGNLLLVAVWCNKIRLIDN
                     IYLNS"
     gene            11531..11881
                     /locus_tag="PLK75_06290"
     CDS             11531..11881
                     /locus_tag="PLK75_06290"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOY38892.1"
                     /translation="MNIEIVKSKIHKVTVTEANLHYVGSITIDEKIMNAANLIVNEKV
                     QVINLNNGERLETYVIKGEYGSGVICLNGPAARKVIVGDVVIIISYASMDFEEAKKFK
                     PSLVFPDTATNQLL"
     gene            11881..12363
                     /gene="rfaE2"
                     /locus_tag="PLK75_06295"
     CDS             11881..12363
                     /gene="rfaE2"
                     /locus_tag="PLK75_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOY38893.1"
                     /translation="MEHTKELYNRIVNRAELVALVSEWKLQGGKIVFTNGCFDILHRG
                     HVEYLHKAADLGSRLIIGLNSDESVKRLKGNSRPIQSEADRALIIAALRIVDAVCIFG
                     EDTPEELIRAVSPDFLVKGGDYVAEMIAGYSWVRSYGGQVLTIPIVDGYSTTGILQKL
                     "
     gene            12432..12857
                     /locus_tag="PLK75_06300"
     CDS             12432..12857
                     /locus_tag="PLK75_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38894.1"
                     /translation="MKYFVVLIIAFSASLVFLSGCAKVEQDVIGEWKSELMKDNPTAE
                     VTWEFKTDGTLVRTYIDNPSNTTEVDECFYSVEKKLTKTLIKIDGSINVSEYENLNGI
                     WQVEYFNDNMMKLRRIDKVLDTKDREDGSYLYREFTRVN"
     gene            12861..14219
                     /gene="radA"
                     /locus_tag="PLK75_06305"
     CDS             12861..14219
                     /gene="radA"
                     /locus_tag="PLK75_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOY38895.1"
                     /translation="MPSYKSVFVCQQCAYKSAKWIGRCPSCNSWNSFIEETVVKKPEN
                     AAKNTSFSAAELQELSEVNPVLCERIVLPFGEFNRVLGGGLVKGSVILIGGEPGIGKS
                     TLIMQSVLGLNNKKVLYVSGEESPSQIKLRADRLSQTGSQMYLFSGIALENILAAART
                     VKPDIVVIDSVQTIYSEDFMSVPGSVSQIRGCAQQLQQFAKSGGVPVILIGHINKEGN
                     IAGPMTLEHIVDVVIQFEGDSNHYYRLLRSKKNRFGSAFELGVFEMTQVGLVEITDPG
                     NMLLSENIQPVSGVSITAMNEGSRTIILEIQALAGNSVYSSPQRTATGFDSRRLNMLL
                     AVIEKRLGYRLGQKDIFLNIAGGIRLNDPAADLAVISAILSSLTDLPISHDTCFIGEI
                     SLSGQVRPVSNIQMRLSEVSKLGFRKVFLSKYQQHLEQQQGVEYVKISSIKEFAGLLF
                     AG"
     gene            complement(14844..17222)
                     /locus_tag="PLK75_06310"
     CDS             complement(14844..17222)
                     /locus_tag="PLK75_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOY38896.1"
                     /translation="MSTSKFTKKTGNKLNNNNISTPEFRKKFLKYFWSTYIIIVFLTI
                     IYFVLISAGALGFMPNFYQLENPNNNLASKIISSDQVVLGTYFRENRSQISFVDLSPH
                     LVNALIATEDIRFYRHSGIDFRALGRVFSGIATGNTKGGGSTITQQLAKMLFPREGDI
                     SKLGFMNRKFREWVIAVKLEKSYTKEEIIAMYFNRFDFLNLAVGIESAAKVYFNTTPD
                     SLTIPQAAMLVGMAKNPSLYNPLRRPEETLKRRNVVLAQMKKYDFITKEQFDSLKNTG
                     LGIKFQKVDHNIGSATYFREFLRMWLTAEKPIESNYVDKRMYVEDSINWYTDPSYGWC
                     NKNVKADGSNYDIYSDGLKIYTTINSHMQKYAEEALTEHMSTYLQDEFFKDQKKRKKA
                     PFAEDMTAEQIDNLMTLSMKRSERYRVLKAENMPEADIIKSFNTPAHMSVFSWKGDID
                     TVMTPMDSIKYYKFYLRAGFMSMEPQTGYVRAYVGGTNYQHFKFDQVSVARRQVGSTF
                     KPFIYCLAMQNGYSPCYKVPNIPVTFELPDGSGQKFYTPKYSPSDKDGAMISLKYGLA
                     GSLNQISAWVLKQFSPEAAVNLAKKMGVYSHIDPYPSICVGSAEVLLKEMVGGYCTFA
                     NKGIYTRPMYVTRIEDKDGNVLCTFNPIQKEAISEETAYLMLDLMRGVVDFGTSVRIR
                     YKYNLKNDLAGKTGTTNNHSDGWFIGIAPQLVSGAWVGGEERSIHFRTITLGQGAVMA
                     LPIWALYMQKVYADKSLPYSTEKRFERPDKISVETDCETYDVQNQETPFIEF"
     gene            17318..19603
                     /gene="topA"
                     /locus_tag="PLK75_06315"
     CDS             17318..19603
                     /gene="topA"
                     /locus_tag="PLK75_06315"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOY38897.1"
                     /translation="MEENLVIVESPAKAKTITKFLGKNFVVKSSFGHIRDLSKKNLGI
                     DVEKKFKPDYIVSPDKKKLVSELKKMSAEAKTVWLASDEDREGEAIAWHLADELKLKR
                     EKTKRIVFHEITKEAIVHAIENPREIDINLVNAQQARRILDRLVGFEISPILWRKIKP
                     ALSAGRVQSVAVKLIVEREREICAFNSESKYRVNAIFEANGKRFKAELSTKFDTLDEA
                     RDFLQSCLNSRFSVKNITVKPGKKSPAPPFTTSTLQQEAGRKLNFSVAQTMRLAQTLY
                     ETGKITYMRTDSVHLSNMALGTIKNTVISEYGEKYLKTRQFQTKAKGAQEAHEAIRPT
                     YSDKKQIEGTKPEQKLYELIWKRTIASQMADAQVEKTIIEVEINNSKYKFVAEGEVIV
                     FEGFLKLYNESSEDENGEEKQGILPKLTIGDELAAQELSSTEKYSVHPPRYNEAGLVK
                     KLEELGIGRPSTYAPTISTIQQREYITKESKKAEERNINCLIIKNNKVEQFVNIEKFG
                     AESNKLFPTSIGILVTDFLSESFPDIMNYNFTAAIEKQFDEIAAGSMEWTSMIDNFYK
                     TFHPKVETTLSKKEVQRYERNLGAHPQTGEPIFVKLGKFGAFVQMGENSGNKPPQFAS
                     LKKNQNIETITTEDALELFKMPRDLGLYNEKPIIIGIGKFGPYIKYNNKFYSLKKAKD
                     DPYTISFDRSVEIINENSNDIFPRTFPEKPGLTILKGRFGPYISYDRKNYKIPRDFDP
                     ITIELEDCLKIIGKNQVDNDK"
     gene            19936..20898
                     /locus_tag="PLK75_06320"
     CDS             19936..20898
                     /locus_tag="PLK75_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOY38898.1"
                     /translation="MRRFLSTITLAIIASVLFAQNDPHFTLFEFNQLTYNPGYAGSNN
                     GICVSSVHRQQYVGFNRDPLNGGKSGRPITTVFNAEMPINSIKSGVGISIIQDMIGCQ
                     SDLAVNLSYAYRLELGYGSLGIGLNLGFINRAIDGDWITPESLNGGAVYTDPSVPHSE
                     SKIAFDMGFGAFFKYDRLYAGLSFAHLLQPTINFDIEKPSYIKRHYYLVAGYNYQLPN
                     PIFEIRPSLLMQYDGATTQFTLNGQFVYSKKIWGGVSYRYNDAISPMLGIYLMNGLSF
                     AYSYDIELSPLSTYSSGSHEIMVRYCFGLDINSDPGRYRSVRRL"
     gene            20964..21392
                     /locus_tag="PLK75_06325"
     CDS             20964..21392
                     /locus_tag="PLK75_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY38899.1"
                     /translation="MRKSLVIVSIFTLFLTACGGYYGSGDLVGVQRKNWYTPDPHGML
                     FIPPGSYQMGPSDQDVPNAMTAQTKTVTLQGFWMDETEITNSEYRQFVYWVRDSLAMT
                     VIAADNSNTSLATYTADQFFVDYSNSKDPAVADFFDESLQ"
BASE COUNT         6316 a         4088 c         4285 g         6733 t
ORIGIN      
        1 gttaatgttc caatattgta gccaatgagg ctactgttgc ctcctgaggt aattcccatc
       61 tgaatactgt cgggtactcc ccatgtatac cagttgatct gtaagctgaa aaaggtccat
      121 tcggtattca ctccggcaaa catggtatcg gcagaagcaa taatttctgc attgtttgta
      181 agccggatgt aaaaaagcgc agtatctccc tccgccagat tatattttat atatccgctt
      241 atggtttgtg gtaccccggt ataaggctct cctggaataa catttaagcc ttgcattact
      301 ccaatagttg ctaatccggg ttgaagcccc ataatagagt ttttcgtctc aaggcgtaag
      361 gcatagtttc ccgaataaac atccgaggtt tctttggttg cgtttaacgt aacccatccg
      421 gtagggctat ccccaataat tgaagtggtc catacctcaa aatccagatt tgtgtatgcc
      481 tgcgaaaaag ttagttttgg taaggatcca aatagcagta ttataacaat aagcgtgtat
      541 agttttctca taatgcgttt tatttaccaa attataacaa aaattatgca aaaatgttct
      601 ttctggcaga ttgtaaaaaa aaaagaaaga atatttggta atcttgataa aagtgatccc
      661 agaagggttc gaaccttcga ccccctgatt aagagtcagg tgctctacca gctaagctat
      721 gggacccaga gtttcaaaaa aatgtaccgg ctgctccggt tttgtggtcc cactagggct
      781 tgaacctagg accccctgat tatgagtcag gtgctctaac caactgagct atgggacctg
      841 aatctgctct tcaaattcag agtgcaatat tatatttttt tttactaata ttcaaattaa
      901 gcgtaactat aaattttgaa tgcttttttg taggttccca agaattgatt tttcaacaaa
      961 agaccttatc attgcaattc agatgattga aaattttcct cgtggtatgc accggtgttt
     1021 catatagcgg aatgttgcat ttaattatgc aatttacttt gatatttatt gttattaaga
     1081 actaagccaa acgtggaaat ttcatttact ttgaaaatat tttataaaac cgatgctaat
     1141 aaaaaatgtg gtattcgatc tggggaatgt aattctgaac ataaatccgg aattatcggt
     1201 gaaagagttc tcgaaacttg gaataccaaa cttcgacaaa ttatatacct tgtcgctgca
     1261 aaccgagctg ttcgatgatc tggaaaccgg aagaacaaat gccggtgcat tttgcgaaaa
     1321 agtaagaatc attacaaaca ccaatttgca tgacagcata attatcgatg cgtggaatgc
     1381 cttgattctc gattttccac aggagaatct tgagttattg aaaagggcgc ggaataaata
     1441 tcgtacattt atcctgagca atactaatag aatacactat gaatattaca ccgagaacct
     1501 gaataaatac catgatatac aaaatctgga atctttggta gaaaaggctt ttttctctca
     1561 cgaaatcggg ctgcgaaaac ccgataaagg tatcttcaat tatatgatta atattacagg
     1621 catcgatcct tctgaaactc ttttcattga tgactcaaga gctaattgtc gcactgctga
     1681 aatgttaggc ttttttgttg aggaataccg cgaaaaaaat ctcccgcaat tattccgcaa
     1741 actaaattta ccattctagg gttctgctat catctttgtc aatgataatt tttaccatta
     1801 ttgtttccgg cggtaataat tcaagcgctt tttcgggcca ttcgataaaa atttttgctt
     1861 ttcccgaaaa atactcttca attccaatat cgagcaattc aacggtattt ttaattctgt
     1921 aaaaatcgaa atgatatatt aagactttgg catcggtttt atattcattt acaatcggaa
     1981 aactagggct gtttaccatg ttctcaacac caagaagttt acataatgct tttataaatg
     2041 tggtttttcc tgcgcccatt tcaccggtaa aagcaacgat tcggttggtt ttggttagtt
     2101 ccaagaactg ttttgcagct ttttcaacac cagcgattga ttttattctg ataacaggca
     2161 ttttatttgc gaggacttaa gtgaataaca gggatcaaca tttcttcgag cgacacgcct
     2221 ccgtgttgaa aactgtcgcg atagtattta acaaagtgat taaagttatt atgatacgcg
     2281 aaaaaatcgt taccggttgc aaagatatac gatgaactca ggttggttct tggaagataa
     2341 attgattcag gtttcagcac ttcaaacact tctttcgggt tgtagttcag attgcgcccc
     2401 tgtttgtacc tcaaatttgt tgttgtattg cgctctccga taacttttat tgcattctgc
     2461 actttaattg ttccatgatc ggtagttatt accagattta cccccagttc cgataatgaa
     2521 cgcaacaatt caagcaatgg ggagtgctta aaccaggatt gagtaatcga acggtaagcg
     2581 gtttcgttgc caaccagctc ctttatcatt ttttggtcgg taactgcatg agaaaaataa
     2641 tccacaaaat ttacgatcat aaccatgaaa tgggaagtgt caacaatttc acgcactctt
     2701 tcgtttactt ttcttaaatt tttaatatca ttaatttttt caaaaaacag attgtcggat
     2761 atgttgtact tgcgcaccag tttttttagc atatcttctt cataaaggtt tttaccgcct
     2821 tcttcttcgt cgtttttcca aagctcaggg tacagtctgt ctatttccga aggcatcaat
     2881 ccactgaata gcgaattgcg tgcatattga gttgatgtgg gaaggataga atagtacata
     2941 tcctctgttt cggttttata aaactcattt aattcaggtt gaattacttt ccattgatcg
     3001 aaacggagat tgtcgataac cagtagaaaa gtttttttac cggatgaaac ctgagggaat
     3061 actttttctt tcagcagact gggcgaaaga attggcttat tgcccgaact ggatccgaac
     3121 caggaagcgt aattcttttt aatatatttt gtaaaggcac tgttggcttc atttttttgc
     3181 atttgtaata cctcatccat tgccgattcg gcagattgcg acagttccag ctcccagtaa
     3241 acgagcttct tgtacacatt tatccagtcc tgatgggtta gagagtcgtt gatttccatt
     3301 cccaatttcg aaaaagccat ctgatattct gaatttgttt tttgggcaat caacctgcgg
     3361 tggtcgatgt tcttttttat cgagagtaga atctgattcg ggttaacggg ctttatcagg
     3421 taatcattta ttttcgaccc aattgcatca tccattatgt tttcttcttc gcttttggta
     3481 atcataatta cgggaaccaa tggctgaatt tcttttatga cttttaacgt ttcaatgcca
     3541 tttagtccgg gcatattctc gtcaataaaa aagatatcgt attctttttt ctttaccatt
     3601 tcaatggcat cgtaaccgtt gttggccgtt tcgagttcat agcctttttg ctgaaggaaa
     3661 agaatgtgcg ggcgaagcag gtcgatttca tcatcaaccc ataatatgct gattttcttc
     3721 attttttagt taaattattg tcagagtaaa aataaatcaa tacgtaaacc gggttttaag
     3781 gatttacata gttggtttcg aaaaagtcaa gatataagtt gatattttta acttttttca
     3841 gagcctgata gttttcttca gagatggtaa attgcttata atcgttgctt tcgattcggc
     3901 caaagacatc gggattgttt ttaataatct tcatataatt aagcgattgc cttttattgt
     3961 cgaaagtttt tacaattacc atctgatact tatcatcgaa aagcatgttc atcaccgtga
     4021 agttcttaag gctgaaggtg aaaatattga aattccttac ttcaaagctc aggcgattaa
     4081 tatcaatctg atcggcattg gcaagtatta tatagtaatg cggaacactc tcattatttt
     4141 taaaaacagc ttcggctgca attagggaat cggaagtatt ggtattaaaa atcaggtttg
     4201 cgtcatcatt tactatagaa ggtcttgctg ccagctcttt gcgcaattct tcaatgtttg
     4261 ctgaaccaaa ataggagaga atgtcctgcg cagtgcgggc aagttcatcg ttcgggtaat
     4321 cgccgataaa atcaaccagc gctttcttaa aactcatttc atccattgtt ttgccaacgc
     4381 aaagtgcgga aataaagcga aatctggctt caaggtcttt ctcggttttc cccgaaaaat
     4441 acttgttggt attggtgtta accaattgac atttgccctg cacatatgca tcatacgtgt
     4501 tcatatacag tgctatatct gactcccgct ttgcttttag ctcatttaca tagttcgggt
     4561 tttgaagcag attggcataa tgcgaagttg gaaattttgt gatgataagg tttcgataag
     4621 tctccatttc agaagcattt cccttaattt tatgcagaag gtataaattg tagtacgatg
     4681 tcagcaggta gatattattc ggaaacctgc ggttcagttc attgaagcaa aatatcgact
     4741 gatcgaaatc ttcgaactgt tctttataaa ttctgccggc attatagaac gcatccatga
     4801 tgcgcgaatt tgaacttgca atcagagaat cggttaatgg cagatcctga aggtaatatt
     4861 cgcgtttttt attgtcgcta acttttctag tgttaagtgt gtcggaggta aggctatctg
     4921 tagatgcagt ttcattgtcg aagctcacta cacttttgtt ttttcgtctc cagtgatctt
     4981 cgtttttgcg gtttccccat tttttaacaa actcattacg tccgaagctt aattgcgagg
     5041 ggttatagaa ataccattta cctcccgacg gagctgaaac attattctgg tatccgtctc
     5101 tgcgttgatc gaacagcatt gaattcatct gttgttcctg ctcgagttca cgttggcgat
     5161 tttcttcctc aattaaatcg gcaataatat cgtcgataag tttattgcgg tctttttcac
     5221 tcatttttgc aactttctga aggctgtcct caagggtaat tgtattcagt tcaatcacca
     5281 gatcattcag gttgttgctc aaatcctgaa tttccttata cgatttgtaa tcaagcggaa
     5341 ggtttgcaag gcaggtgtcg taatatgcct gagcatttat atagtctttt ctgccaaaga
     5401 atatctttcc caatttctca caagaaagtg ctttctgata gttgtttgcc atactttttt
     5461 ctgaggacaa cttgtagttt ttaatggcat catcagtttt tttgtcccta tagtcaattt
     5521 cggcaatagc ataataaatc tgatcgagat attctatatt cttatcgtcc ttgagcattc
     5581 tccttagctt tttacggact tctttcccac ctgtaccgct atataaacgg gcaatgttta
     5641 tgcgtgcatt gaattccatt tcgtaaacgg gattcttttt tgcgaccgcc gcatacattt
     5701 ccgatgctct ttttgcatca ccggtagctt cgtagagttg agccaaaata tacatgtaac
     5761 gaatacgtgc actttttttt ccggcatatt ttatggcttt cttcagtttt gggatagcat
     5821 catcgtattg cccaagtttt atgtaataat cagcataggc agctgcaaat tgcccctgat
     5881 atttcttggg cattgattta tcggcttcaa tcaggtcaaa tatttcttta gcactcttat
     5941 acttctgact ttcgctgaaa gttctggcta gccatatttt agcatcatag gcaactagtt
     6001 ctttggggaa ttgccgaatg agatattcga aattctgcct tgcttcgaaa aaatcgtgtt
     6061 ttgcaaaata ggccataccc attaaaagat aggagtcgtc gacccatttg caaaactcct
     6121 ttttattata aaactccttc tcttttgcac tgagcttacc cgattttgtc ttgggcttgg
     6181 cggtaattga gtggaatttt atggctttcg acgatttttt tattgttttg tccatttcac
     6241 ccaccagcga atttgcagca tccggtttgc tgataataaa catgggaagt attgaaatgt
     6301 agctgtcggt attggaagct tctgcctgtt taatgccttt tttataagat tcctttgcat
     6361 taaaataaat attgtagcgt gctgtggtgt tgtggtaagt tcttgataaa aaggtgtttt
     6421 tttctgtgga gcaactccag ccaagcagtg agagatacag taaaccgatt acaatatata
     6481 cactggtttt cgactgtgct atgttaaatt tcagatgcat tttaatgtaa cagaaaattt
     6541 cagtttttat tattttgatt tttattattc tacagtaacc gatttggcaa ggttccgggg
     6601 ttgatctaca ttacatcccc gcattaccgc tatgtaatac gacaaaagct gcaaaggcac
     6661 aacggtaagc aaaggggcta atgagcaatc ggagtaaggc acctcaataa catgatctgc
     6721 catattttta ataacagtat ctccttcggt tacaatagca ataacatttc ctttccgtgc
     6781 ttttacttcc tgaatattgc ttacaatttt ttcgtatgat ttgtcttttg tggccaaaac
     6841 aataaccggc attttttcat cgataagggc aattggtccg tgtttcattt cggctgccgg
     6901 ataaccttca gcgtggatgt atgaaatctc cttgagctta agagcacctt caagggcaac
     6961 aggaaacaag tagccacgac caaggtagag ggcatttgtt gcatctttat ataattcagc
     7021 gatgtttttt atttcatccg acttggcaag aattgattca attttaccgg ggatttcaaa
     7081 taactcttca attaaatcat catattcttt ttccgtcagg cttttattct gtttaccaac
     7141 cagcattgcc atcattatta gtacagtaac ctgagctgta aaggcttttg ttgatgcaac
     7201 gccaatttcg ggtccggcat gggtgtacac tccggcatga gtttcacggg gaatgctgga
     7261 gcctacgacg ttgcatattc ccagcaccaa tgctcctgaa tcttttgcaa gtcgtattgc
     7321 agcaagagta tcggcagtct ctccgctttg gctgatagca attactacat catttttgtt
     7381 gatgattgga ttacgataac ggaattctga tgcatattca acctcaacag gtatgcgggc
     7441 aaattcctca aacaaatatt ccccaaccaa agctgcatgc cagcttgtac cacaggcaac
     7501 aatcagtact ctgtcggcct cgatgagttt tgaaccaacc gcatgaagtc ctcccaactt
     7561 gagcgatttg tcgtaataat tgagcctacc acggcaggtg tcggtaattg aacggggctg
     7621 ctcgaatatt tctttgagca tataatgatc gaatcctccc ttttcaattt cctcgatgtc
     7681 cagctcaatt ttttgtattt caggcttttt ggtaatatta cttacgcttt tcagatgaag
     7741 ccctgattta ttaacaatgg caatatcgtc gtcattcaaa tagatcacgc tgtttgtata
     7801 ctcaataatt ggggtggcat cggaggcaaa aaagtattca ttctgacctt tacctattac
     7861 cagagggctc cctttacgag ccgcaataat tgtattggct ttttgagtaa atgccactag
     7921 aatcccatag gctccgatta cttttgtcaa ggcaactctt acagcttcct cggggggtac
     7981 ttccagtttc ctgtaaacat attctataag gtttgcaagt acctcggtgt cggtttcgct
     8041 aataaaggta tatccttctt caacaagttc gttgcgtagc tggttataat tttcgattat
     8101 tccgttgtgt ataacaacaa attgcttgtt catggaaaga tgagggtgtg catttacatc
     8161 agttggttcg ccatgtgttg cccatcgtgt atgacccatg cctatattac cgcttgtatc
     8221 ctgatcggcg accagattct cgagatcaga tactttccct ttacatttgt aaattctagt
     8281 tttgctgtta agcaaagcaa tccccgcaga gtcgtagcct ctgtattcaa gccttttcag
     8341 ccctctgatc agcactgtgt aagcatctct gtaacctaaa tatccgacga ttccacacat
     8401 ggtattatta gaattttgaa tacatgatta ttaacttcat tgaatttaca ggactatcgc
     8461 ccccgtacac agttacacgg cttgcatcag tgcggttatc aagggggaat agaaatattc
     8521 cgttgtcaat cagattttgg tcggaaaaga gctcctgcaa atgaaatggt atgttaaatg
     8581 tgtaggtata gtccgattca tcaaggactc cgccaaaata cgatgaattt attttgtagt
     8641 ccgttagaaa tgtatttaag ccatcttcgt tttcggctac aagagttagt tttggcggag
     8701 gagcaaatcc caattcgtta gcccggttta cggtaatctg caattgagcc ttgtttactg
     8761 catatcggca tgtgtcgaaa ccttcgaggc ctttgatgta cagtttcact tttaaaccgc
     8821 ctaacgactg aagaaaactt actgtgttgg gttcattcag gttatcaatg gtttgttgga
     8881 tttctgctga ggctagagaa taatcgtgtt caaacatatt gatgcgagca gagcttgcat
     8941 tgataaggaa atcaaaactc aggctatcat tataatataa agtcatttcg gaatttgacg
     9001 aaatcaggtt aaagtatagt atggcgcctc ctgcattcac attatctgtt gtaatataca
     9061 agcctttgaa gaaatcgaga aatgtatcgt tgctaacaaa tgatgaaaca ttggaagagc
     9121 tgatgaagcg gttgattaaa tcatcgggca tctttatatt cacaaaactg tcggatggat
     9181 gaggagttat ctccactgag catatctccg ataaatcgcc gggattaaac ctaaagttcg
     9241 aataatacgt tgaatcgggg taaatgtcct gatttaggac ttcataaacc ctgattcttt
     9301 gtgttgttgt tgtgtcgccg taaaaaccag tatagaaaag gcgcagtacc aaagagtcgg
     9361 ctgtttcaac aacattcgaa aaattaacat ttgaactcga taacctcaaa tgggtaaaga
     9421 aatccgcttt acaaaagccg aaaaccggat ctatgtaaga gcctaaagga cttagtgtcc
     9481 tttcgtcgga tgcgatactg tcgtcaagaa cagtataagt ttccacttcc agtgtatcgc
     9541 agagtaatcc cattctgtcg gagccgggtt gcaaatttaa tcctatttcg tttggctctc
     9601 tttcgcacga gtacataaga aaaataattg aactcaaaat gaacgcttgg ctgattacct
     9661 tactccacat ttttattccc gtcattgcta tttccgtgta attaaatcgt aaaatttgtt
     9721 gtatgcataa atataatcat ccggaccttg atattcaagt actggctttc cggaattact
     9781 aatatattcg tgaaggtctt cgtgaatgtt tttactaccc ataattacac cgttgctgta
     9841 ttcaatggct agtttattaa ggtttacata gtttgcttct tcaactactg caacatcttc
     9901 tttcgaaatg ccttccgaca tgattttttg cgaaaagtca gcactgagct cttttttgaa
     9961 atcttcgtta taaaccgaat aaataatttt caggtcacta aataaagggt catccgcata
    10021 ggttttgcga atatataaag gtaccaaagc cgaaaaccaa ccgtggcaat gcaccagatc
    10081 gggtgcccag cataattttt ttactgtctc aatcacaccc cttgcataaa aaatggctct
    10141 ttcatcattg tccgcgaaaa aagaaccttt cttatctctc agagcgaatt tccgcccgaa
    10201 ataatcttcg ttatcaataa aatacacctg catgcgagcc ggctgaatcg aagcaacttt
    10261 tattatcaaa gggtggtcgg tatcgttaat taccatattc ataccgctca gacgaatcac
    10321 ttcatgaagc tgatttcttc gttcgttgat acaaccatac tttggcataa aagttcttat
    10381 ctcacgccct tgctcctgaa caccctgcgg caggtaacga ctgataacag atatctctga
    10441 ttccggcaga tatggggtga tttcctgaga tacgtaaaga acctttaaac tcatacttat
    10501 attcttgata agttttaatc tgcaaaatta gtaaaataaa tcaatttctc aaaaaaccaa
    10561 tttttgcaca ataaagcttt ttgttatttt atataaggtg tataacgatt aaattctaat
    10621 tttgttttgc aaaattgata actcatgaaa gtttttacat cgcatgaaga tttgaattat
    10681 tgtttgtcag aatatagatc tgggggattg acgattggct ttgtccccac tatgggggct
    10741 ttacacgccg ggcatatttc attgattaag aagtcgactg gccaaaacaa ggtctcggtt
    10801 gtaagtgttt ttgtaaaccc gcgccagttt aacgattcaa atgattttac aaattatccg
    10861 agaaatattt cggcagacat taaattaata gaagcagctg gttgtgaatt tgtgtatacg
    10921 cctgattatc aggatttatt ccccactgaa gattcacttg aatttgactt taatggactt
    10981 gataaagttt tggagggggc ataccggcct ggtcactttc agggtgttgt cgatgtggtg
    11041 catgcattgt ttaaagccgt ccgccccaac aatgcttatt ttggtttgaa ggattaccaa
    11101 caattgaaga taattcaatt gcttgcttcg caatttcacc ccaaaaccaa cattatacct
    11161 tgtcctatta taagagaacc ggacggactt gcaatgagtt ccagaaatca acgcctgcaa
    11221 ccggacgaaa gaaaggctgc ggcaatgata cctaaaatat taatggaatc tgctgcgttt
    11281 actggaaatc actccgtggc tgagctgaaa gctaatatag tttcccgatt caacaaggcc
    11341 gaaggcttaa agcttgaata tgccgaaata tgcaactccg ataatttaga agcaatcgat
    11401 tccggcaatc ctgcaaaagg aaatctgttg ctggttgctg tatggtgcaa taaaatacgc
    11461 ctgattgata atatttattt gaactcataa aagaaatatt cttacatttg cgacttcttt
    11521 ttagggtgtt atgaatatag aaattgtaaa gtcgaaaatt cacaaagtta ccgttaccga
    11581 agctaacctc cattatgttg gcagcattac cattgacgag aaaattatga atgcagccaa
    11641 cctgatcgta aacgaaaaag tccaggtaat taatctgaat aacggtgagc gcctcgaaac
    11701 ctatgtgata aaaggcgaat acggctcagg tgtgatttgt ctcaatggcc ctgctgcccg
    11761 aaaagtaatt gtgggagatg tggtcattat tatttcgtat gccagtatgg attttgaaga
    11821 agcaaagaaa tttaagccat cccttgtttt tcctgatact gcaacaaacc agcttctctg
    11881 atggagcata ccaaagaatt atataatcgg attgtgaatc gggcagagct tgttgcattg
    11941 gtttcggaat ggaagcttca aggcgggaaa attgttttca ctaatggctg tttcgatata
    12001 ttgcacaggg ggcatgttga atacctgcat aaagctgctg acctgggttc aagattgatt
    12061 atcggattga actccgacga gtcggttaag cgactgaaag gcaatagccg gccgattcaa
    12121 agcgaagctg acagagcttt aattattgca gcgcttagaa ttgttgatgc cgtgtgtatt
    12181 ttcggagaag atacccccga agaattaatt cgtgctgtta gccctgactt tctggtgaaa
    12241 ggtggcgatt atgttgctga aatgatagca ggctacagct gggtgcggtc gtacggcgga
    12301 caagtgctta ccattcccat tgttgatgga tacagcacaa caggtattct ccaaaagtta
    12361 taaacaaaat ttgtgattgt gaattctttt tatatatatt tgcataatgt cctaaaaaat
    12421 aagagattac tatgaaatac tttgttgtac tcattatagc tttttcggct tctttagtct
    12481 tcctttcagg atgcgccaag gttgaacagg atgtaatagg tgaatggaag tcggaactga
    12541 tgaaagataa tccaactgca gaagttacct gggagtttaa aactgatggt acattagtaa
    12601 gaacgtatat cgacaatcca tcgaatacaa ctgaggttga cgaatgcttt tacagtgttg
    12661 agaaaaaact taccaaaacc cttattaaaa tagatggctc tattaatgtt tctgaatacg
    12721 aaaatttgaa tggtatatgg caggtagagt attttaatga taatatgatg aaattgcgcc
    12781 gtattgataa ggttctcgat accaaagatc gtgaagacgg ttcatattta tatcgcgagt
    12841 ttacccgagt aaactaaaaa atgccctctt ataagtctgt ttttgtttgt cagcagtgtg
    12901 cctataaatc tgccaagtgg ataggccgtt gcccctcatg caattcgtgg aattctttta
    12961 ttgaagagac agtagtaaag aagccggaaa acgctgcgaa gaatacaagc ttttctgcag
    13021 ccgaacttca ggagctctcc gaagtaaatc cggttttgtg tgagcgcatt gtgcttcctt
    13081 tcggcgagtt caacagggtg ttgggagggg gactggtaaa aggctccgta attcttattg
    13141 gaggcgaacc cggaatcggt aaatcaacgc ttattatgca atcggtgctg ggacttaaca
    13201 acaaaaaggt actctatgtt tcgggagaag aaagcccatc gcaaattaaa ctgagagccg
    13261 acagactttc gcagaccggt tcgcagatgt acctattttc gggaattgcc ctggaaaaca
    13321 ttttggcagc agcccgcact gttaaacctg atattgtagt gattgattcg gtacagacaa
    13381 tttattccga ggattttatg tcggtacctg gttcggtaag tcaaatacgc ggctgtgccc
    13441 agcaattgca gcaatttgcc aaatcaggag gtgttccggt aatacttatc ggacacatca
    13501 acaaagaagg aaatatcgct ggccccatga ctctggagca tatcgttgat gttgtgatac
    13561 agttcgaggg cgacagtaac cactattacc gtttgctgag aagtaaaaaa aatcgctttg
    13621 gctctgcttt tgaattgggg gtattcgaaa tgactcaagt cggacttgtt gaaattactg
    13681 atccgggaaa tatgctcctt tccgaaaaca ttcaaccggt aagcggggtt tcgattactg
    13741 caatgaacga aggtagcagg accattattc tggaaataca ggcactggcc ggtaattcgg
    13801 tttacagcag cccccagcgt acagcaacag gcttcgactc caggaggttg aacatgcttc
    13861 tggcagtaat tgaaaaaagg cttggctaca gactgggtca gaaagatatc ttcctgaata
    13921 ttgcaggtgg tatcaggttg aatgatcctg ctgccgatct ggctgtgatt tctgctattc
    13981 tttcctcact taccgatctt cctatctctc acgatacctg ttttatcgga gaaataagcc
    14041 tgtcagggca ggtgcgcccg gtttcgaata ttcagatgcg tttatcggaa gtaagtaagc
    14101 tgggtttccg caaagtcttt ctttcgaaat atcagcagca tctagaacaa cagcaaggtg
    14161 tagaatatgt gaaaattagc agcatcaagg aatttgcagg gctacttttt gccggttaaa
    14221 attcaataaa aggtgtttca tcctgaatta ttcttgtaca gaaagtaatc ataaatggca
    14281 tattaacaat aaattgcgct tccgtaaacg attatttttc tgtactaagc ttatactaac
    14341 ctttggtgta atatggtatg aatatcatac ctcatggcgt aatgttattc ataaaaatcc
    14401 gtgttcccaa ttgcatttcc attcacacag ccacctaagt tgacaagttc actgtaggca
    14461 acgcgggcag ccccgacaaa aatgcgagcc aaccggggcg tgaatacccg tttaatagaa
    14521 aaacaacaaa gggcaagcga agcaaacacc ccactaatct gactccggtt tgcttcacgc
    14581 cgctttgttt tgtcggacaa aggagaaggg cctgagctga caaaacacta ctttgttagt
    14641 ttttctttaa acgggtagtg tggggagata gcatattttt gtcttgattt tttgttactt
    14701 tttcattaag gaaaaagtaa gagaaagaat ggcttataga ttttttacga cccaatcgtt
    14761 aacaatggtc accgagtttt ggcttaaaat tgacgcatta aattggataa gtaaggagtc
    14821 attttcaagt tgctttttgc cggttaaaat tcaataaaag gtgtttcctg attttgcaca
    14881 tcgtaggttt cacaatcggt ttccacactt attttatcgg gtcgttcaaa tcgtttttca
    14941 gtgctatacg gcagcgactt atcggcatat actttttgca tatacaatgc ccagatagga
    15001 agggccatta cagcaccctg cccgagtgtt atcgtgcgaa agtgaatgct cctttcttcg
    15061 ccaccaaccc atgctccgct taccaattgt ggagcaattc caataaacca tccatcggaa
    15121 tgattattgg tggttccggt tttgccggcc aggtcatttt ttaaattgta tttgtaacgt
    15181 atccttacac tggtgccaaa gtcaaccact ccccgcatca aatccagcat aaggtaagca
    15241 gtctcttcgc tgatagcttc tttttggatg gggttgaaag tacacagtac attaccatct
    15301 ttatcttcaa tccgggttac atacatcggc cgggtataaa tgcctttgtt ggcaaaagtg
    15361 caataacctc ctaccatttc tttaagtaaa acctctgctg acccaacaca aatgctgggg
    15421 taaggatcga tatgactgta aacccccatc ttcttggcca gatttactgc tgcttcagga
    15481 gaaaactgct ttagtaccca ggcggaaatc tgattcagcg atccggccaa tccatatttt
    15541 aagctgatca tcgcgccatc tttatcggat ggggaatact taggtgtgta aaatttctga
    15601 cccgaaccat caggtaattc aaaagtgacg gggatattgg gaaccttata gcagggtgaa
    15661 tacccatttt gcatagcaag gcagtaaata aatggcttga atgttgaacc aacctgccgg
    15721 cgggcgactg aaacctgatc gaatttgaaa tgctgatagt tggtgcctcc aacataagcc
    15781 cttacatagc ccgtttgcgg ttccatcgac ataaacccgg cacgtaaata aaacttatag
    15841 tatttaatcg aatccatggg agtcattact gtatcaatat cacccttcca gctgaagacc
    15901 gacatatgtg ccggagtatt gaaactttta atgatatctg cttccggcat attttcggct
    15961 ttaagaacac gatagcgctc actgcgtttc atcgacaatg tcataagatt atcaatctgt
    16021 tctgcagtca tatcttcggc gaatggggct ttctttcgct ttttctgatc tttaaagaac
    16081 tcatcctgca aatatgtgct catatgctcg gtaagcgctt cttcagcata tttttgcata
    16141 tgcgaattta tggttgtata aatttttaat ccgtcactgt aaatgtcgta attgctgccg
    16201 tcggccttta cgttcttatt gcaccagcca tatgatggat cggtgtacca attgatggag
    16261 tcttcaacat acattctttt atcgacataa ttgctttcga tgggtttttc agcagttagc
    16321 cacattcgca ggaattccct gaaataagtt gccgacccga tattatgatc gactttctga
    16381 aattttatgc ccaagccggt gttttttaaa gaatcgaatt gctctttggt aatgaaatcg
    16441 tattttttca tttgggcaag aacaacattc cgtcgtttca gggtttcttc agggcgacgc
    16501 agtgggttat agagtgatgg gttctttgcc atgccaacaa gcatggcagc ctggggaatt
    16561 gtcagcgagt cgggagtggt gttgaaatac actttagccg ccgattcaat ccctacggca
    16621 agattcagaa aatcgaaacg attaaagtac attgctatga tttcttcttt agtatagctt
    16681 ttttcaagct taaccgcaat aacccattcc ctgaatttgc gattcataaa ccccagtttc
    16741 gaaatatctc cttccctcgg aaatagcatt tttgcaagtt gttgtgtaat agtactccct
    16801 cctcctttgg tatttccggt tgcaattcct gagaaaaccc ttccgagtgc acggaaatcg
    16861 atgcctgagt gacggtagaa acgaatatct tcagtagcaa ttaaggcatt aaccaaatgg
    16921 ggcgacaggt cgacaaaact tatttggctt cggttttcgc gaaagtaagt acccagcact
    16981 acctgatcgg aagagataat tttactcgcc agattgttgt ttggattttc gagttgatag
    17041 aagttcggca taaacccgag agcaccagca gaaattaaaa caaagtatat aatggtaaga
    17101 aatactatta ttatataggt actccaaaaa tattttaaga attttttacg aaattccgga
    17161 gtgcttatat tattgttatt aagcttgtta cctgtttttt ttgtgaattt ggaggtggac
    17221 atagcatgta aatttgatgc aaaaataaat aataatttgg aagttatttt ttgaattgcg
    17281 ttaatttgca tcgcaattcg aaattataaa acatccgatg gaagaaaatc tggttattgt
    17341 agaatctcct gctaaagcta aaacaattac aaaatttcta ggtaaaaatt ttgttgtgaa
    17401 atcgagtttc gggcatatcc gcgacttgtc gaaaaagaat ctgggcatag acgtagaaaa
    17461 gaaatttaaa cccgattata ttgtttctcc cgacaagaag aaactggtta gtgaactgaa
    17521 aaaaatgtcg gcagaagcaa aaacagtttg gctggcatcg gatgaagacc gagaaggtga
    17581 agcaattgca tggcacctgg cagatgaatt aaaactgaag cgcgagaaaa ctaaacgcat
    17641 agtatttcac gaaatcacaa aagaagctat tgttcatgct attgaaaacc cgcgcgaaat
    17701 tgatatcaat ctggtaaatg cgcaacaggc gaggagaatt ctcgacagac tggtgggatt
    17761 tgagatttca cccattcttt ggcgtaaaat taaacctgca ctttcggcag gaagggtgca
    17821 atcggttgcc gtaaaactga ttgttgaacg tgaacgcgaa atatgtgcat tcaatagcga
    17881 gagtaaatac agggttaatg caatatttga agcaaacgga aaacgcttta aggctgaact
    17941 cagtaccaaa tttgatactc ttgatgaagc gcgcgacttt ctgcaatcat gcctgaattc
    18001 ccggttcagc gttaaaaaca ttacggtaaa accaggaaaa aaatcgcctg caccaccatt
    18061 tacaacatct acactgcagc aggaagccgg acggaaactc aatttctcag tagcacaaac
    18121 catgcgcttg gctcaaacat tgtatgaaac cggaaagata acttatatgc gaaccgactc
    18181 cgtacattta tcgaacatgg ctttgggtac tattaaaaac accgttatca gcgaatatgg
    18241 tgagaaatat ctgaagacac gccagtttca gacaaaagcc aagggagcgc aggaggccca
    18301 tgaagcaatt agaccaacct attccgataa aaaacagatt gagggtacca aaccggaaca
    18361 gaaactgtat gagctgatct ggaaaaggac aattgcatca caaatggcag acgctcaagt
    18421 agaaaaaaca attattgaag tcgaaattaa taactcgaaa tataagtttg ttgctgaagg
    18481 tgaagtaatt gttttcgagg gcttcctgaa attatacaat gaatcaagtg aagatgaaaa
    18541 tggagaagaa aagcagggca tattgccaaa gctgactatc ggggatgagc ttgctgctca
    18601 agaattgtct tcaactgaga aatattctgt gcaccctccc cgctacaatg aggcaggtct
    18661 tgttaaaaag cttgaagaac ttggtatcgg aaggccatcg acctatgcac caactatttc
    18721 aacgattcaa caacgggaat acataactaa agaaagtaaa aaagcagaag aaagaaatat
    18781 caattgtctt atcattaaaa ataataaggt agaacaattt gttaatattg aaaaatttgg
    18841 agccgaatcc aataaactct ttcctacttc aatcggaatt ttggtaaccg atttcctttc
    18901 ggaaagtttt cctgatataa tgaattacaa ttttacagca gccatagaga agcagttcga
    18961 cgaaattgct gcaggaagta tggaatggac ttcgatgatt gataattttt ataaaacatt
    19021 tcatcctaag gtggagacca cgctttcgaa aaaagaagta cagcgttacg aacgcaatct
    19081 gggagcgcac ccgcaaacag gagaacctat atttgttaaa ctgggcaaat ttggtgcttt
    19141 tgtgcaaatg ggcgaaaact ctggcaacaa acctcctcag tttgccagtc ttaagaaaaa
    19201 tcaaaatatc gaaaccatta ccaccgaaga tgcacttgag ctattcaaaa tgccccgtga
    19261 tttaggttta tataatgaaa aaccgataat tatcgggatt ggaaaatttg gcccctatat
    19321 aaaatataat aacaaattct attctctgaa aaaggcaaaa gacgatccct atactatttc
    19381 ttttgatcga tcagtagaaa taatcaatga aaactccaac gatatattcc caagaacttt
    19441 ccccgaaaaa ccaggtttaa ctattctgaa aggaagattc ggtccctata tttcgtatga
    19501 taggaaaaat tataaaatcc ccagggattt tgaccctata acaatcgaat tagaggattg
    19561 tttaaaaata attggcaaaa atcaggtaga taatgataaa taaagtttgc taataagtta
    19621 aaataatgaa gttatagagg caaaatattt gataaaaaat actaaacctg ttttgtttgc
    19681 gttgtaataa aaaatattat ttgtattttt tttactaaaa acttgataaa taaaataatt
    19741 attgtaaatt tcgcagtttg atagtttatt gaattatttg ttggcagaac gaaaatagtg
    19801 attatagtta ttaacaattt ttgagttatg aacaaaaaaa tgttaataaa atttggatat
    19861 taataaaaat tatattttct ttaccgcatg ttgatggtta aagatacaga ttgagaaagg
    19921 attaaaaata tgattatgag gagattttta tcaacaatta ctctggctat tattgcaagc
    19981 gtgctgtttg cgcagaacga ccctcatttc actctttttg aatttaacca gttgacatat
    20041 aacccgggat atgcaggaag caacaatgga atttgtgttt catcggttca caggcagcaa
    20101 tatgttggtt ttaaccgtga tcccctgaat ggagggaaaa gcggaagacc cattaccacc
    20161 gtgtttaacg ctgaaatgcc aatcaattct ataaaaagtg gtgtgggaat atctataatt
    20221 caggatatga ttggttgcca gtccgatctt gctgtaaacc taagttatgc ctacaggctt
    20281 gaattgggat atggcagcct tggaatcgga ttaaatctgg gttttataaa cagggcaatt
    20341 gacggtgact ggattactcc tgaatcgctc aatggcggag ctgtatatac cgacccaagc
    20401 gtacctcatt ccgaaagtaa aatagctttt gatatgggct ttggcgcatt tttcaaatat
    20461 gatcgtttgt atgccggctt gtcatttgct cacttgttgc aaccaactat taatttcgac
    20521 attgaaaaac ccagttacat aaaacgccat tattatttgg ttgcaggtta taattatcag
    20581 cttccaaacc caattttcga aatcagacct tcactgctga tgcaatatga tggagctact
    20641 acacagttta cattaaacgg acaatttgtt tacagtaaaa aaatatgggg tggtgtgagt
    20701 taccgttaca acgatgctat cagcccaatg ttgggtattt atttaatgaa cggcctaagt
    20761 tttgcatatt cttacgacat agagttatct ccgttaagta catattcgag tggatcgcac
    20821 gaaattatgg tacggtactg ttttggtctg gatattaatt cagatccagg aagatacaga
    20881 agtgtaagaa gattgtaact taattttatt atttacgtaa aaaatgttgc tttttaattt
    20941 tgtcgcacaa ataaaacaat aatatgagaa agtcactggt gattgtttca atctttactc
    21001 tgtttttaac cgcctgcgga ggctattatg gcagcggcga tttggttgga gttcaaagaa
    21061 aaaactggta cacccccgat cctcacggga tgctgttcat acctcccggt tcatatcaga
    21121 tgggtccgag cgatcaggat gttcccaatg ctatgacggc acaaaccaaa acagtaaccc
    21181 tgcagggttt ttggatggat gaaaccgaaa ttaccaattc ggagtaccga caatttgttt
    21241 attgggtgcg cgactctctt gcaatgactg tgattgctgc tgacaactca aacacatcgc
    21301 tggcaactta taccgccgac cagttttttg ttgattattc taattcgaaa gatcctgctg
    21361 ttgccgattt ctttgatgaa agccttcagt gatatgaata tgatatggat ggttttagat
    21421 tt
//
LOCUS       DAOOQP010000044        21256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3037_length_21256_cov_5.179520, whole genome shotgun sequence.
ACCESSION   DAOOQP010000044 DAOOQP010000000
VERSION     DAOOQP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_21256_cov_5.179520"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..459)
                     /locus_tag="PLK75_06330"
     CDS             complement(91..459)
                     /locus_tag="PLK75_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOY38900.1"
                     /translation="MKGILTIVLLICSNIFMTLAWYGHLKFSQMTWFNKLGLIAIVLI
                     SWGIALFEYFFQVPANRIGYQGNGGPFSLFQLKVIQEVITLVVFTLFTLLVFRTETFR
                     INHLIGFIFLVLAVFFIFKK"
     gene            complement(558..1493)
                     /locus_tag="PLK75_06335"
     CDS             complement(558..1493)
                     /locus_tag="PLK75_06335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY38901.1"
                     /translation="MSLKWKSKALVQKTISFFPFKEKLNYFFQKNVTRGLDLDDVHFG
                     YKIQAASDHLNYLRKYTDFSLWKSVSVLELGTGWYPVVPVSMYLCGIGKITSIDIRSW
                     SSKKSVFACIYKFIEWHKSGELHKYLPDIDQGKWKKLVDLVAEGSDFSLYDFYALIGM
                     RPIVCDARRTGLDDTSFDFICSNNTFEHIHQEVLCDILKEFTRLLKKDGLMSHQIDMT
                     DHFAHFDKSITVFNFLKYSIRQWKIIDNAIQPQNRLRLVDYKKMYEGLNIPVTEEKIW
                     RGKPEDLQRVSVSNDFKDYSVEELVITHAYLISKF"
     gene            complement(1642..4593)
                     /locus_tag="PLK75_06340"
     CDS             complement(1642..4593)
                     /locus_tag="PLK75_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38902.1"
                     /translation="MRKLTLFTLLVLLVSGGIILAVSQQRNAQAVYQPDNTTMQLQHN
                     SFSGAAKWIYERQANVQTGQINPGAVENAIRQIGRMQSSRNAMGLNWQFAGPSNIGGR
                     CRAILVDEDNTDIIYAGGVSGGLWKSTTGGTSWNQVKYAGDNETALIPNLNISSICQT
                     SNGDIYFGTGEFFANPYGSQNTGFQGAGIWKSTDGETFTRLSATWSTSDSKNTFVFVN
                     KMVAHPSEPNTVYAATKRGLQMTTDGGLTWTNPILNIAGFPINEVCGDVEISSDGNSI
                     IVDLGIQVYVSHEAGASDSWVKVTGSDEGELPTTSIRTELAIAPSDKNIMYAQTTKSD
                     RSLLNIYRSTDGGLTWSIIGPGGSTEFNPLGEQGDYDNIIAVFPDNANEIMVGGQYSM
                     WKWGLSTGWEMLSYWSLDIASPMYVHADQHEIRFNPNNPDIVYVGSDGGIHRSINRGN
                     TWTTLNKNFGVTQFYAIGHDGTNNLIGGTQDNGTLFIDPDRTITSGLQNEAVEVSGGD
                     GGYSEISQIEPSILFSTIYYGSLYRSEDNGETMAPFYNARLSNQLNPGAEESGHSFVT
                     PISLWESFNDLNSIEMLTKIADRDYAAGEIVYVESNIKEHYVKKVLDEPLAHDDTLEV
                     ADHYQSRLAVGFKGSVWITKDALNFRVSANWIPVAKVRNETNNYEVVQCLEWSADGAY
                     LYFATNWINGGFFNGSVLYRVGDFNVHRKDSARDADFSTYLLEVAPIAVFNDRHITGI
                     GVDPEFSGNVVVSLGNYDNTNYVYYSTSANTAPAVATGPGSFVSKQGNLPAMPVYDAL
                     IVWSDSRKVIVGTEFGVFATSDITAANPVWTDENGEGMDYVPVYHLRQQIIPNGWMED
                     VHLDNGIRNHGYIWAGTHGRGIFKSTEFEGPVGICSNVADKGNLNLLIYPNPVTEIAN
                     LAFEITENTTNARLSLYNLEGKLVKTFDLNNVTKGKYQKQINVTDINNGVYMVVLTYG
                     NTRTSAKFIKR"
     gene            complement(4706..5548)
                     /locus_tag="PLK75_06345"
     CDS             complement(4706..5548)
                     /locus_tag="PLK75_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38903.1"
                     /translation="MGICVKGMMKGSTYIFCIILFFFVSVIGNYLYAQGEIETEREPD
                     NYNENSFGFKLNSNGFGADYRFSKRINFKLRYFYEAQVNYIKDPKEIRVINPYFEVSK
                     KFVYGKMNSFQNLKLGFGINNMFIEKQDKGSISVHAIFGAGGILGVEKPVYYELVDSS
                     KDAGDYVVYYTNIHRMDIHKYNPTDVYSKAPFKYGLDEIKIVPGIFVKSAIAFDLSKN
                     VMKTNVIEGGVEFDVYFRKVEIMAENDKNLFLYVYLSYRFGSKYNAVLSREVRKSQRK
                     EQLN"
     gene            complement(5904..9704)
                     /locus_tag="PLK75_06350"
     CDS             complement(5904..9704)
                     /locus_tag="PLK75_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38904.1"
                     /translation="MKKLYILSVALFVFSLGLSAQSTPDNWTVATSLITVSEETTTVS
                     EGAKAMKVVFTGTANEDIRSESFSVTGGASFVYTLDVYDNDAAGRVRMAIIWSSANDY
                     STIYSADLATFQTLTYSGTVPAEATSAYVLLRFYDISGNWDGDAELIIDNASFTENAG
                     INLIQNPSFELWSVLVIPEYTIAEIQGNTGASPYDGDMVITSGIVTGVFATGYFIQDG
                     AGAWNGIFVYDNAHDPLLGDSLTIKAIVDEYNGKTELTSVSEYNVNSVDNTLPDAVVV
                     TTLAAAGELYEGVLVKVENAACTNEDAGFGEWTVDDGSGAINIDDFIFAYTPTLGTTY
                     SITGIVDYANSAYKILPRSLDDIVELGNPIEIAIADAQNNSSLTVEPNPITVLHGEDV
                     SFDVTIQYATFSPDLPTGYYVDAYISFTDPLMAGLNINIVHTGYGIDVNYAPEAGTSV
                     LTLSQMLGTSPALLSGHSGITDVWTITITGLGVGQYQAEIASLCDDNTDGPYSTFILA
                     SDVLDVFVTSNIVDALYEVFEASSLTITPESQVITQGNDAVLTAVAVYPETIDPILSE
                     YLTDALITFEDVTPSGATVDIVYDDNMGGHADLGSFELPSGIYEIYLSELIESARTPL
                     SGHAGLTITWSITIHNLPQAYYIFHMNTVTNTEAGFGTEDYLLADDWAIITVQEAQDE
                     LIIESQSPDMIVCEGTEVVLTVVVTGAENITYAWYNGETLLGFTENSITVSEEGQYVC
                     IATAEELQAISDTMDVTISIPMPELGENASYCTGYEVILNPGIFMGYDWGSSVEPTLT
                     VTENGEYFVTVSDEYGCTAVDSVEVAFAEELTLNLGEDIYICPDGSVEISSPIPGTYA
                     WSNETVENSILVTEAGTYSLTVTQASCTATDEIEVIQASNPEEFDLGEEINLCIGAEA
                     ELTSPVEADSYLWNTGEETNSITVTETGLYTLTIYNEHGCSVSDDIQVNFNNFIIVNL
                     GDDIESCHGQTVVLDPLMGISWLWSDSSDADTLAVTESGDYSVTVTDQYGCSGSDEIN
                     VYFRDLPVIELANDTTICDGFSITINAVDNMTYLWNTGETTQEIIADNMGNYIVTITD
                     EFGCSNSDSLFLTVLPVPVVDLGPDNTISEDQTVILGNYPGYASYLWSNEAVTDYIVI
                     NGAQLGVGEHLFWVLVTSANGCETYDEVLITVVEGIGVDPEMIAQLAVYPNPASAMLN
                     IDFGSVTGEKAVSLFDATGKTVLSEVTGSGLLKMDISSLQQGLYIIRITTNGITITRT
                     VVVQ"
     gene            9901..11583
                     /locus_tag="PLK75_06355"
     CDS             9901..11583
                     /locus_tag="PLK75_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013671760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY38905.1"
                     /translation="MTEAIKATLRESRTARWTALVLLAFAMFCSYIFMDILSPIKDLM
                     QSTRGWDSAAFGTMQGSETFLNVFIFFLIFAGIILDKLGVRFTAILSGAVMLFGAVIK
                     WYSVSTAFVGSGIETWFTENLNYIPGFDELGISPFYRGMPASAKLASVGFMIFGCGVE
                     MAGITVSRGIVKWFKGKEMALAMGSEMALARLGVATCMIFSPLIAKWGADATSDTFLM
                     DVNVANSVAFGVILLMIALIMFIVYFFMDKKLDAQTGEAEEKDDPFKVSDIRKIFAST
                     GFWLVALLCVLYYSAIFPFQKYAVNMLQCNLTFVEPEAGTFWASNTVTFVQYGIMLIV
                     AAFAFISNFRKGAQKYIFLGISIIALIAFCYMGYMRQSAETIFAVFPLLAVGITPILG
                     KYVDTKGKAASMLMIGSILLIVCHLTFAFVLPLAKGSAIGGVLIAYVTILVLGASFSL
                     VPASLWPSVPKLVDQKVIGSAYALIFWIQNIGLWLFPLLIGRILNSSNPDIVKQLDAG
                     TMTQQEAAVSYDYTNPLIMLAFLGVAALIIGFLLKYIDKKKGYGLELPNISK"
     gene            complement(11650..13110)
                     /locus_tag="PLK75_06360"
     CDS             complement(11650..13110)
                     /locus_tag="PLK75_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOY38906.1"
                     /translation="MNALNHLEPKLVWQFFEEITKVPRPSKKEEKIIKYLLDFGKKHG
                     LETKRDKAGNVLISKPAAKGFETRPTVVLQSHMDMVCEKLAKVEIDFDNDPITPVIDG
                     DWVKAKGTTLGADDGMGVAAQLAILASSGIQHGKIECLFTVDEETGLTGAFALEKGFI
                     TGKILLNLDSEDEGEMFIGCAGGVDTVAVFDYFSTDIPKGAVSFEMQVSGLKGGHSGD
                     EIHKGLGNSVKIAARFLHDISKKFPVYLSYIDAGNKRNAIPREALVKFAVDSKNVGKL
                     KASFKTYVATVKAELERVESKLEVTMLRTHSQKKAVDQSTLKNLLMALMACPHGVINM
                     SPAIQGLVETSTNLASVKMPKGKNKKIVVTTSQRSSLESGKKYVRDMVHSVFELAGAN
                     IKHSDGYPGWAPNPQSEVLKVVVKSYEDLFGVTPVVRAIHAGLECGLFLEKYPDLDMV
                     SFGPTLRDVHTPDERVNIPSVEKWWKHLIDVLKKIK"
     gene            13203..13850
                     /locus_tag="PLK75_06365"
     CDS             13203..13850
                     /locus_tag="PLK75_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOY38907.1"
                     /translation="MRTHFSTAFLFFALLLAFSSCEKKRQNIFSGIVTETITGTPVEG
                     VSVEIEMKSVGNNGFVSGFETIGTAVTDAEGRFSFVCDPAQALKFRLTYSKDGYHITT
                     REITPDDVVGTYTESVDLPREAYLSYHFINLPPNPDSDVFRYRVTGMADGCDVCCSNE
                     YHSFYGQNDTVILCPVIAYDSLIVEFYTFLNGVSHQYTDQIIGVPSDTVEKTIRY"
     gene            13852..14619
                     /locus_tag="PLK75_06370"
     CDS             13852..14619
                     /locus_tag="PLK75_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY38908.1"
                     /translation="MNLKLIIQTVFVSLFFMSQSQKVFNQNRTPNIIPIKAGCNCYLI
                     CSNDGAVLIDAGSHHKLQAFDKVLAQNGLTWDTIKYIIITHTHYDHVANLAEIKKRSG
                     AKVIIHKNESSFIESGITPLPEGMTFTGKIGICYTRHLGKHNVKVCPAAAEISVEDNY
                     TIPGFGYRIIYNPGHSSGSISLILNDNICFCGDAAFAVLGNNLMPITGECKTGIVESW
                     IKLLDTGCTIFYPGHGKPFTFKELQKSLQKYKKLLSQ"
     gene            complement(14619..14690)
                     /locus_tag="PLK75_06375"
     tRNA            complement(14619..14690)
                     /locus_tag="PLK75_06375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14656..14658),aa:Thr,seq:cgt)
     gene            complement(14738..15631)
                     /locus_tag="PLK75_06380"
     CDS             complement(14738..15631)
                     /locus_tag="PLK75_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38909.1"
                     /translation="MKMKRNIQPAKVSKVDKAIFFIISGIWIAILVFGIITLLQPEWL
                     KSISVDGRRTESNHSMELGNVEMYKGNMLGAISNYQQAFMVDTSNYEALGNLGIAFTY
                     NGQYSEALSSFEKLRIKLKQDYRLEIFYQNMGDLYERMNRPDSAFYYYYKAVETGITS
                     PYVYRKAGLFASKLDNDSLAIVMIEKSAELIKSLEYYYEEAVFVGYQEAIRKNDTVNI
                     KVFGRFLSEVGNVENMSRYDSEILTYWLTESKDLGFAYLYLAQIAAKNGRYEEASKYA
                     NLCQKYYPSLTPELQKLFPKP"
     gene            complement(15628..16836)
                     /locus_tag="PLK75_06385"
     CDS             complement(15628..16836)
                     /locus_tag="PLK75_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOY38910.1"
                     /translation="MKFKFSANLNLTLYSLLLIVTPFLMLMNFLQDAIGKLSNAHFTV
                     YDVKITYLLFIAILGLIFVFIRVRKELTKTRILGLFLMVALTLIGQKIGDYYYNHNFY
                     DLQHNWHYIAYGIFSYVAYRKFFLKNFSPAKIIWRTFLMAFIISLFDEIIQVYISNRV
                     FDLSDVGKDMWGALIGQFFIQFVLLECKHIKPFTYRHQKIKDYITSPSSALFIQAGVS
                     LIFIYISSVLSYPSLWPQVFLISLLAVFFFLLILHMGNTRLLKWIIRGTFGIVVIWLV
                     YANFSGNAYVKYVSENVIVYKGLPFYYFDVMIFPDGGYRVVDKKETFLGRDKLKIDDI
                     NPDILIIGTGKGNTGGKGFNDQRLFEFRPNFTHSNLYQIIKLPNKQACLKYNELLSKN
                     KKVLLILYQE"
     gene            complement(16966..18057)
                     /locus_tag="PLK75_06390"
     CDS             complement(16966..18057)
                     /locus_tag="PLK75_06390"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOY38911.1"
                     /translation="MKQNIFEPGDEVRFLDEIGSAVVVRNLYDGMVLVRTDDDFEYPV
                     HPGQLILIKKNTKTASEIATERVVTPHTGSKTNTIGKAADVKKTASEINTVEHNLNEK
                     LRISLAFTPVLPNDPVNGDLLMHIVNDSGYELLFQVLKQDDTGIRTIAKGNADPGFIE
                     DLFLFNREGLNQLKKITVQLIFCGKFGMLQRSPIERHYKPDPVCFFTSGNYAANSYFD
                     TKALLWDIINEQEPDAATVEKIHKLADLLSEKEAPPENKAEKYAAREKPKTIEVDLHI
                     NAILDDCKGLSNAEILDYQMQHFHKSLTDAMFEKAQKVVFIHGIGNGTLRDKVRESLK
                     TQYHLNFQDASFQEFGFGATLVFLSGKQL"
     gene            18156..19352
                     /locus_tag="PLK75_06395"
     CDS             18156..19352
                     /locus_tag="PLK75_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOY38912.1"
                     /translation="MSEFGYNLPDDRIAKYPASERDSSKLLVYRDAEIQEYLFCNLPE
                     ILPENGLILFNNTKVIHARLQFRKETGAAIEIFCLEPTTPSDYQISLSAQQSCTWKCL
                     IGNKKKWKKDALKKLFRINNRDITLSAEIVSDTNNESLIRFSWDTTDCSFAEILEHEG
                     EIPLPPYLNRNAEPSDTIRYQTVYSRFDGSVAAPTAGLHFTDTVLAEISRKNITCSEL
                     TLHVGAGTFKPVSESNVENHTMHREHFSVSVETLNQIRNNLGNITITGTTSLRAVESL
                     YWLAGRIQRGETAAHLSQWEPYTFQNEISAADAIDILLEYCQKQNIAAFSASTSVMIL
                     PGYQFRFADRLITNFHQPKSTLLLLVAAFIGNDWKKVYDVALNHNFRFLSYGDSSLLF
                     KANNWL"
     gene            complement(19378..19563)
                     /locus_tag="PLK75_06400"
     CDS             complement(19378..19563)
                     /locus_tag="PLK75_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38913.1"
                     /translation="MGKFKMQTSKQINIARNTAGRKNWQTDYDDRIIRNDAEYQSIVE
                     YIRNNPGACECVFTNKT"
     gene            19833..20636
                     /locus_tag="PLK75_06405"
     CDS             19833..20636
                     /locus_tag="PLK75_06405"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOY38914.1"
                     /translation="MEQRKYNYIEEYLNKIRAAGGFTFTYSELLGTFNVTKEAIDLSL
                     QRLKARNSIAQIKKGFYVILTPEYAQKGMIPSALFVDDLMKSLNKKYYVGLLSAAALH
                     GASHQQPLEYFVITQAPAIRDINASNMRVRFFVKKDWHPQSVVQIKSDSGYMNVSSPE
                     LTALDLFFYSHYISTNRAYTVLQELQEAMKPSKLKSTALLFPQTASIQRLGYILEQIQ
                     GCDKLADSLKKVLNTRKSYYIPLSSGKLKKGSVNTRWKIIINTELDGDL"
     gene            20633..21223
                     /locus_tag="PLK75_06410"
     CDS             20633..21223
                     /locus_tag="PLK75_06410"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOY38915.1"
                     /translation="MIPKRYIEEWKQTVPWIDNNQVEQDLIISRALVAIYSDSFLMEN
                     LAFRGGTALHKLYANPASRYSEDIDLVQIKPGPIGDVITQLRKALLFIEGFKPTIDRG
                     VAMTTLRFRYVSESFTNVNMKLKVEINCREHIAVYGLKHFNYSVSNSWFTGSAQLITF
                     EPEELLATKLRALYQRKKGRDLFDLWYLLTKLLPIP"
BASE COUNT         6337 a         4450 c         4030 g         6439 t
ORIGIN      
        1 aacacctttt gggtttttcc ttgatttaca tatcgatgcc cccggatatt cgcttgcaag
       61 gatatttaaa agcttttaaa agaaagaatc ttatttctta aaaataaaaa acacagcaag
      121 tacgagaaaa ataaacccaa tcaggtgatt gatcctgaat gtttctgtac ggaaaaccag
      181 tagagtaaac agtgtaaata ccacaagcgt tattacctcc tgtatcactt tcagctgaaa
      241 caacgaaaac gggcctccgt ttccctgata tcctatgcga tttgccggta cctgaaaaaa
      301 atactcaaaa agtgcaattc cccaactaat gagcactatt gcaataagtc caagcttgtt
      361 gaaccaagtc atctgcgaaa atttcaggtg cccgtaccat gccagggtca tgaagatgtt
      421 ggagcaaatc aacaatacaa tcgtgagaat tcctttcatc tgtatttata attttttaat
      481 tgttcatacg gaatctcgca taaaatagta aacgttttgg tgggtgatat ttttatcatg
      541 ctcgatctca taatacatca gaatttgctt attaaatagg catgggttat taccaattcc
      601 tcgacgctat aatctttgaa atcattagag acagagaccc tttgcaggtc ttcgggcttc
      661 ccacgccata ttttttcttc agttacagga atattcaggc cttcatacat ttttttgtaa
      721 tcaactaaac gaagtcgatt ctgtggctga atcgcattat cgattatttt ccattgacgt
      781 atgctgtact taaggaagtt aaaaacagta atgcttttat cgaaatgagc aaaatggtca
      841 gtcatatcaa tttgatgact catcaaacca tcttttttta atagtctggt aaattctttc
      901 agaatatcgc aaagcacctc ctggtgtata tgctcaaatg tattgtttga acaaataaaa
      961 tcgaagcttg tatcatcgag gccggttctc cttgcatcgc acacaatagg tctcatccct
     1021 atgagggcat agaaatcgta caacgaaaag tcgctacctt cggcaaccag atcaacaagc
     1081 tttttccact tgccctgatc aatatcaggt aaatatttat gcaattcacc tgatttatgc
     1141 cattcgataa atttgtaaat acaggcaaaa acagactttt tgctactcca cgatctgatg
     1201 tcgatagaag taattttacc aattccgcac aaatacatcg aaaccggaac aacaggatac
     1261 cacccggtgc cgagttcaag taccgaaacg ctcttccata aagagaaatc tgtatacttt
     1321 cgcaggtagt tcaggtggtc ggatgcagcc tggattttat atccaaaatg cacgtcgtct
     1381 agatccagtc ctctggtaac atttttctga aaaaaataat tcagtttctc cttgaaaggg
     1441 aagaatgata tagtcttctg taccagtgcc ttagatttcc attttaacga catacagcct
     1501 tatgtgagtt gcgggataaa aatataaaaa ttaaaataaa ccacatcatt aatacgtcaa
     1561 tgaatcaccc taaaagaata ggttgaatgt tataaaaaag gcactccggg ttggaatgcc
     1621 ttttttcgaa ttcaaaacac attatcgctt aataaatttg gcagaggtac gggtattacc
     1681 ataagttaga acaaccatgt aaacaccgtt attaatatcg gttacgttga tttgtttttg
     1741 gtactttccc ttagtcacat tattcaaatc gaaggttttc accagtttac cctcaagatt
     1801 atataggctg agtctggcat tcgtagtatt ttcggtaatt tcaaaggcca ggttggcaat
     1861 ttcggtaacc ggattcggat aaattaataa attaaggttt cctttgtcgg caacgttact
     1921 gcaaattcct acagggcctt cgaattcagt ggatttaaaa ataccgcgac catgagtgcc
     1981 tgcccaaata tagccatgat ttctgatacc gttatcgaga tgcacatctt ccatccaacc
     2041 attggggata atttgctgac gaaggtgata cacaggcaca taatccattc cttcaccatt
     2101 ctcgtctgtc caaaccggat tggctgcagt aatatcgctg gttgcaaaaa ctccaaattc
     2161 cgtacccacg attactttgc gtgagtcgct ccatactatc agtgcatcgt acaccggcat
     2221 tgcaggcaga tttccctgct ttgaaacgaa actccctgga ccggtagcta ccgccggggc
     2281 tgtattagcg ctggttgagt aatacacata attggtatta tcataattac ctaacgatac
     2341 cacaacattg cccgaaaatt cagggtcaac gccaattccg gttatgtgtc ggtcgttgaa
     2401 aacagcaatc ggagcaactt cgagcaggta agttgagaag tcggcatctc tggcgctgtc
     2461 ttttctgtga acattaaaat caccaacccg gtacaaaacc gaaccattaa aaaatccgcc
     2521 attaatccaa ttggttgcaa aatacaggta ggcaccatcg gcgctccatt caagacattg
     2581 aactacttcg taattattgg tttcgttgcg aacctttgca acgggtatcc agtttgcaga
     2641 aacacggaaa ttgagcgcat cctttgttat ccaaacactg cctttaaagc ctactgccaa
     2701 acgcgattga taatgatcgg ccacttccag ggtgtcgtcg tgtgctaaag gttcatcgag
     2761 aacttttttc acataatgtt cttttatgtt actttctaca tacacaattt caccagcagc
     2821 ataatcgcgg tctgctattt ttgtaagcat ttcaatgctg ttcaggtcgt tgaaagactc
     2881 ccacaagcta ataggtgtaa caaatgagtg tccgctttct tctgcaccag ggttgagctg
     2941 attagagagt cgtgcattat agaaaggagc cattgtttcg ccattatctt cggaacggta
     3001 caagctacca taatatattg tgctgaataa aatacttggt tctatctgtg atatttcgct
     3061 gtaaccacca tcgcctcccg acacctctac tgcttcattt tgaagacctg aagtaatagt
     3121 acgatccggg tcaataaaca gagttccatt atcctgagtc cctccgatta agttattggt
     3181 tccatcgtgg ccgatagcat agaactgggt aactccaaag ttcttgttca atgtcgtcca
     3241 ggtgttacct ctgtttatag aacgatgaat tccaccatcg ctgcccacat acactatatc
     3301 ggggttattc ggattaaagc gtatttcgtg ctgatcggca tgtacataca ttggactggc
     3361 aatatcgagg ctccagtacg ataacatttc ccaaccagtt gaaagtcccc atttccacat
     3421 gctgtactgg ccaccaacca tgatttcgtt tgcattatcg ggaaaaacgg ctataatatt
     3481 gtcgtaatcg ccttgttcgc caagaggatt aaattcggtg ctacctccgg gtccgataat
     3541 tgaccaagtc aagccaccat cggtggaacg gtaaatgttg agcaatgagc gatcggattt
     3601 ggtggtttgg gcatacatga tatttttatc ggaaggagca atagcgagtt cagttcgtat
     3661 tgatgttgta ggcagttcac cttcatcaga accggtaacc ttaacccatg aatcagatgc
     3721 tcctgcttca tgcgaaacat aaacctgaat acccagatca acgataattg aatttccgtc
     3781 ggaactgatt tcgacatcgc cgcaaacttc atttatcggg aagccggcta tatttaaaat
     3841 tggattggtc caggtaagtc cgccatcggt ggtcatttgc aaaccgcgct tggtagcagc
     3901 atatacggtg ttgggttctg aagggtgagc taccatttta ttaacaaaga cgaaagtatt
     3961 tttactatca gatgttgacc atgttgcact cagacgtgta aatgtttcac catcggttga
     4021 tttccatatc ccggctccct gaaacccggt attctgagaa ccataagggt tggcaaagaa
     4081 ttcaccggta ccgaagtaga tatctccgtt tgaagtttgg cagatagagc taatattcaa
     4141 attcggaata agtgctgttt cattgtctcc ggcatatttc acctgattcc agcttgtgcc
     4201 tccagtggtt gatttccaca atccccctga aacgcctcct gcataaatta tatcggtgtt
     4261 gtcttcgtca accagaattg ccctgcaacg gccaccaata tttgatggtc cggcgaactg
     4321 ccagttcaag cccatggcat tacgcgagga ctgcattctt ccaatctgcc tgattgcatt
     4381 ttcaacagct cccggattga tttggcctgt ttgtacattg gcctgacgtt cgtaaatcca
     4441 tttggcagct cccgaaaaac tgttgtgctg aagctgcatt gttgtgttat cgggttgata
     4501 cacagcttgt gcattccttt gctgggaaac cgccagaatt ataccaccgg aaaccaataa
     4561 aaccaacaac gtgaataaag ttaactttct cataataaat tatttacgta aaattttaat
     4621 aaccattgca tatccatttt gcaaactcct aatataattt gattttttta taaaaacaaa
     4681 gaaaaatatt cacacaccaa aatatttaat tcaactgctc ctttcgttgg cttttacgga
     4741 cttctcgact cagaacagca ttgtacttag aaccgaaacg gtaggataaa tacacgtata
     4801 gaaacaaatt tttatcattt tcagccataa tttcgacctt gcggaaatag acatcgaatt
     4861 ctacgcctcc ctcaatcacg tttgtcttca ttacattctt actcagatcg aatgcaattg
     4921 ccgacttcac aaagattcca ggaacgattt taatttcatc aagaccgtat ttgaaagggg
     4981 ctttcgagta tacatcggtt gggttgtatt tatgtatgtc catccggtgt atgttagtgt
     5041 agtaaacaac atagtcacca gcatctttcg agctgtcgac aagctcataa taaaccggct
     5101 tttcaacacc caagatccct ccggctccaa aaattgcatg aaccgaaata ctgcctttat
     5161 cctgtttttc tataaacata ttgtttattc caaaacccag tttaaggttt tgaaaggagt
     5221 tcattttccc ataaacaaat tttttactga cttcaaagta tggattgatt acccgtatct
     5281 ctttggggtc tttaatataa tttacctgag cttcgtaaaa gtaccttagc ttaaaattaa
     5341 tacgtttcga aaaacggtaa tcagcaccaa atccattgga attcagttta aacccaaagg
     5401 aattctcatt ataattatcg ggttcccgtt cagtttctat ttcaccttga gcatacaaat
     5461 aattcccaat gactgaaaca aaaaaaaaca gaatgataca gaatatatat gttgatcctt
     5521 tcatcattcc ctttacacaa atacccatac cttataattc gcatataaga tacagaaata
     5581 atattttaat aatcgtcagc cgttattttc aacttgctgg ggttctgacg agctgtacgc
     5641 cggacaatcc tgggttccct tacacgagta gagaccaatg cataacagtg caagaacagc
     5701 aataataatt aatgtgcttt ttttcatcgt aaaatttatt agaacatcgt ttgaggttta
     5761 ttcaaaccac tataaactac aaaattagtc gtccacaatc ttaattacaa tttattcctc
     5821 gaaattttat taacatagta acgtttaata aaaaaatgga caagggaaag ccctgtccat
     5881 tttataactt attgaattag tttttattgt acaactacgg ttcttgttat tgtgattccg
     5941 tttgttgtaa ttctaataat atataatccc tgttgcagac tgctgatgtc cattttcaac
     6001 aatcctgatc ctgttacttc cgaaagcact gttttaccgg ttgcatcaaa caagctaaca
     6061 gccttttctc cggtaacgga accgaaatca atatttaaca tcgcactggc aggatttgga
     6121 taaacagcca actgtgcaat catctcagga tctactccta taccttcaac aactgtgata
     6181 agtacttcgt cgtaagtttc gcagccgttc gcagaagtta caagcaccca gaacagatgt
     6241 tcgccaactc caagttgggc tccgttaatc acaatataat cagttactgc ttcgttgctc
     6301 cacaaatatg aagcataacc cggatagtta ccaaggataa ccgtttgatc ttcgcttatg
     6361 gtgttatccg gacccaggtc gacaaccgga acaggcagca cggtaaggaa aaggctgtca
     6421 ctgttgctgc atccgaattc atcggtgata gtaacgatat agttgcccat gttgtctgct
     6481 atgatttctt gtgtggtttc tcctgtattc caaaggtagg tcatattatc cacagcgttg
     6541 atagtaattg aaaatccgtc gcagatagtt gtatcgtttg caagctcaat aaccggcagg
     6601 tctctgaagt acacatttat ttcatcggat cctgagcagc catactgatc ggtaacagtt
     6661 actgagtaat cgcccgattc agttacagca agagtatcag catcggagct atctgaccaa
     6721 agccatgata tccccataag cgggtccaga acaacagttt gtccatggca tgactcaatg
     6781 tcatcgccaa ggttaacaat aataaaattg ttgaagttca cttgaatatc atcgcttacc
     6841 gaacatccgt gttcattata aattgtaaga gtgtataaac ctgtttctgt aacagtaata
     6901 gaatttgttt cttctcccgt attccacagg tatgaatcgg cttcaaccgg tgaggtaagt
     6961 tctgcttcgg caccaataca caggttaatt tcttcgccga ggtcgaattc ttcaggattg
     7021 cttgcctgaa taacttctat ttcgtcggta gcggtgcagg aagcctgggt aaccgtaagc
     7081 gaataggttc cggcttctgt aaccagaatg ctgttttcta cagtctcatt actccatgca
     7141 taagtacccg gtatcgggga tgaaatttcg acagaaccat cagggcagat atagatatct
     7201 tctccgagat tgagggttaa ttcttcggca aatgcaactt caacgctatc aactgcagta
     7261 cagccgtatt catcggaaac cgtaacaaaa tactcaccat tttcggtaac agtaagcgta
     7321 ggttcaacgc tgctgcccca gtcgtaaccc ataaatatac cggggttcag tattacctca
     7381 taacctgtac agtacgaggc attttcccca agctcaggca ttggaatcga aatggttaca
     7441 tccattgtgt cggaaatagc ctgaagttct tctgctgttg caatacaaac atattgacct
     7501 tcttcgctga cagtaatact gttttcggta aagcccagca atgtttctcc attataccat
     7561 gcataggtaa tgttttcggc acctgtaact accacagtaa gcacaacctc tgtgccttcg
     7621 caaactatca tatcaggtga ttgcgattca attataagct cgtcttgtgc ctcctgaaca
     7681 gtaattatcg cccaatcatc ggcaagtaaa taatcttcag taccaaagcc ggcttcggta
     7741 tttgtcaccg tattcatatg aaaaatataa taagcttgtg ggagattatg aattgtaatg
     7801 ctccaggtaa ttgtcaaacc ggcgtgtccg gacaaaggtg ttcgtgccga ctcaatcaat
     7861 tcgctcaggt aaatctcata tattccggaa ggtaattcaa atgatccaag atcggcatgt
     7921 ccgcccatat tatcatcata cacaatatcg actgtagctc ctgatggggt aacatcttca
     7981 aaagtaatca gagcatctgt caggtattca ctcaatatag ggtcgatagt ttcaggatat
     8041 acagcaactg ctgttagtac ggcatcgtta ccttgagtta tcacctgcga ttccggtgta
     8101 attgtaagag atgatgcttc aaaaacttca tatagcgcat caacaatatt gcttgttaca
     8161 aaaacatcca gcacatccga agcaagaata aacgtactgt aagggccatc ggtattgtca
     8221 tcgcacaacg aggcaatttc agcctgatat tggccaacgc caagccctgt aattgttatt
     8281 gtccaaacat cggttattcc ggaatgacct gaaagtaatg cagggcttgt gccaagcatc
     8341 tgcgacaatg taagtactga agtacctgct tccggagcgt aatttacatc aataccatat
     8401 cctgtatgta caatgttgat attcaatcca gccattaaag gatcggtaaa tgaaatgtaa
     8461 gcatcaacgt aataacccgt aggcaggtca ggtgaaaaag ttgcatactg aattgtaaca
     8521 tcaaaactca catcctcacc gtgcaaaact gtgattgggt tgggttcaac cgtaagcgat
     8581 gaattattct gagcgtcggc aattgctatt tcgatgggat ttccgagctc tacaatatca
     8641 tcgagcgaac ggggaagtat tttataggct gaattagcat aatcaacaat acctgtaata
     8701 ctgtatgttg ttcccagagt gggtgtataa gcaaatataa agtcatcaat atttattgcc
     8761 cctgacccat cgtcaactgt ccattctccg aaaccggcat cttcattagt gcatgctgcg
     8821 ttttcgactt ttaccaaaac accttcatac agttctcctg cggcagccag tgtagtaaca
     8881 acaactgcat ccgggagggt gttatccact gaattcacgt tatattccga aactgaggta
     8941 agttccgttt tgccgttgta ttcatcaaca attgccttta ttgtaaggct gtcaccaaga
     9001 agtgggtcgt gtgcattgtc gtaaacaaaa attccgttcc atgcaccggc tccgtcctga
     9061 ataaaataac ccgtggcaaa aactccggta acaataccac tggtaataac catgtcgcca
     9121 tcataaggcg aggcgcctgt atttccctgt atttcagcaa tggtatattc cggaataaca
     9181 agcaccgacc ataactcgaa tgatgggttc tgaatgaggt ttattcctgc gttttcagtg
     9241 aaacttgcat tatcaataat caattctgca tctccatccc agtttcccga aatatcataa
     9301 aacctgagta gtacatatgc tgatgttgct tcagccggta cagtaccgct gtatgtaagc
     9361 gtctgaaaag tagctaggtc tgcagaataa atggttgaat aatcgttagc agaactccag
     9421 attattgcca ttctcaccct gccggcagca tcattgtcgt acacgtcgag agtatacaca
     9481 aaacttgctc ctcctgtaac ggagaacgac tcagaacgaa tgtcctcatt ggcagttccc
     9541 gtgaaaacaa ctttcatagc ttttgcacct tcactaaccg ttgtggtttc ttcactcacc
     9601 gtgatcagcg atgtggccac cgtccagtta tcaggggtgc tctgagcact caaacccaat
     9661 gaaaacacaa acagtgcaac cgataaaatg tacagttttt tcataataaa tcattttaat
     9721 ttttcgtgga taaatatatt aaattttgct ttagcgaaaa aaaatattgc cggtttttat
     9781 caattataaa atgaacaatg ctgcaagagt aaaaataatt ttattaaaat cgaaaggttg
     9841 taattgaaaa aatatacatt tgtttgctta tttttcaaag aacaattaaa acttaaagct
     9901 atgacagaag caattaaagc cacattaaga gaaagtagga cggcaaggtg gaccgcctta
     9961 gtgttgttag cattcgcaat gttttgttcg tatatcttta tggatatact ttcaccgatt
    10021 aaagacttaa tgcaatcgac acgagggtgg gattcggctg catttggaac tatgcagggg
    10081 agtgaaacgt ttttaaatgt ttttattttc ttcctcattt ttgcaggtat tattttggat
    10141 aaattagggg taagatttac cgctattttg tctggagctg tgatgttatt tggtgcggtt
    10201 attaaatggt attctgtaag cacagcattt gttgggtctg ggatagaaac ttggtttaca
    10261 gaaaacttaa attatattcc cggatttgat gaattaggaa tttcaccatt ttatagaggt
    10321 atgcctgcat ctgcaaaact tgcatcagtc ggctttatga tttttggctg tggcgttgaa
    10381 atggctggga ttacagtttc acgtggtatt gtgaaatggt ttaaaggcaa agaaatggca
    10441 ttggctatgg ggtcagaaat ggcattggct cgattaggag ttgcaacatg tatgatattc
    10501 tctccactaa ttgcaaaatg gggagcagat gctaccagtg atactttttt aatggatgta
    10561 aacgtagcaa attctgttgc tttcggagta attttactaa tgattgctct gataatgttc
    10621 attgtttatt tctttatgga taaaaaactg gatgcacaaa ccggtgaagc ggaagaaaaa
    10681 gacgatccat tcaaagtgag cgatatccgc aaaatatttg caagtaccgg attttggctg
    10741 gttgctttac tttgtgtact atattattct gcaattttcc cattccaaaa gtatgctgtt
    10801 aatatgctac aatgtaatct aacatttgtt gagccagaag ccggaacttt ctgggcttct
    10861 aatacagtta cctttgttca gtacgggatt atgttaattg ttgctgcttt tgcatttatt
    10921 agcaatttcc gcaaaggcgc ccaaaaatac atattcctgg gaatatcaat aattgccctc
    10981 attgctttct gttatatggg ttatatgcgt cagtcggctg aaactatttt tgctgtattt
    11041 cctttattgg ctgtgggtat tactccaatt cttggtaaat atgttgatac caaagggaaa
    11101 gctgcatcaa tgcttatgat aggttctatt ttattaatag tttgccattt aacatttgca
    11161 tttgtattac cattagccaa aggcagtgcc attggtggtg tattgattgc ttacgttact
    11221 attttagttc ttggagcttc tttctcattg gttcctgctt ctttgtggcc aagcgtaccc
    11281 aaattagttg accaaaaagt aattggttcg gcttatgcgt tgattttctg gatacagaac
    11341 atcggtttgt ggctattccc attattaatc ggcagaatcc ttaattcatc aaaccctgat
    11401 atcgttaaac aactcgatgc tggtacaatg actcaacagg aggcagcagt atcgtatgac
    11461 tatacgaacc cactcattat gcttgcattt ttaggcgttg ctgcgcttat tatcggattc
    11521 cttctaaaat acattgacaa gaaaaaagga tatggactgg agctccccaa catcagtaaa
    11581 taaaatcaac tcaatatcat aaaaaaaggc agccttcggg ctgccttttt tattcaaaat
    11641 gccgatgcac tattttattt tctttagcac atcaatgagg tgtttccacc acttttcaac
    11701 ggaaggtatg tttactcttt cgtcaggagt gtgaacatcg cgcagtgtcg gaccaaagga
    11761 aaccatatcc agatcagggt atttttcaag gaataaacca cattccaacc ctgcatgaat
    11821 ggctcttaca acgggggtaa ctccgaacag atcttcatac gatttcacaa caactttgag
    11881 cacttccgac tgtggatttg gtgcccatcc gggatagccg tcggaatgct taatgttcgc
    11941 tccagcaagt tcaaacactg aatggaccat atcgcgaaca tattttttcc cgctttcaag
    12001 cgaactgcgc tgactggttg taaccacaat ctttttgttt tttccctttg gcatttttac
    12061 tgaagcaagg ttggtggaag tctcaaccaa accctgaatt gcaggactca tattgataac
    12121 gccgtgcgga caagccatca atgccataag caaatttttc agtgtggact gatcgacagc
    12181 tttcttctgg ctatgggtac gcaacatcgt aacttcaagt tttgattcaa ccctttccag
    12241 ttctgccttc actgtggcaa cgtatgtttt aaacgatgct tttagttttc cgacattctt
    12301 tgaatcgacg gcaaatttca ccaaagcttc tcgcggaatt gcattacgtt tattccctgc
    12361 atcaatgtaa gagagatata ccggaaattt tttactgata tcgtgtaaaa agcgggctgc
    12421 gattttcacc gaattgccga gacctttatg tatttcatca ccgctgtgtc cacctttcaa
    12481 tcccgatacc tgcatttcga atgaaacagc gcctttggga atgtcggttg aaaaataatc
    12541 aaacacagca acagtatcaa caccaccggc gcacccaata aacatttctc cttcatcctc
    12601 tgaatcgagg ttcaacaata ttttcccggt aataaagcct ttttccaggg caaaagcacc
    12661 cgtaagtccg gtttcttcgt ctactgtgaa caagcattca attttaccat gctgaattcc
    12721 ggatgaagcc agtatggcca attgagcggc cacacccata ccatcgtcgg cacctaaagt
    12781 agtgcctttt gcttttaccc aatctccatc aataaccggt gtaatcgggt cgttatcaaa
    12841 atcaatttca actttcgcaa gtttttcgca aaccatatcc atatggcttt gcaacaccac
    12901 agtcggcctg gtttcgaaac ctttcgcagc gggtttagat atcagtacgt tgccagcctt
    12961 gtcgcgcttg gtttcaagtc catgtttttt accaaaatca agaagatatt tgataatttt
    13021 ttcttctttt ttcgaaggac gcggcacttt tgttatttct tcaaaaaact gccaaacaag
    13081 cttgggttca agatgattca atgcattcat atttttacat aattatttga tcaaaaattt
    13141 tcaatcaaaa atacatattt atccattatc tttattgtaa ttttgtaata attcagaagt
    13201 caatgcgaac acatttttca acggcatttt tgttttttgc tttattattg gctttctctt
    13261 catgcgaaaa gaaaaggcag aatatctttt caggtatcgt taccgaaacc attacaggca
    13321 ctcctgtaga gggtgtaagc gttgaaattg aaatgaaatc ggttggcaat aatggatttg
    13381 tatcagggtt tgaaacgatt ggcaccgcgg taactgatgc tgaaggaaga ttcagttttg
    13441 tgtgcgaccc tgcacaagca cttaaattca ggctaaccta cagtaaagac gggtatcata
    13501 ttacaactcg tgaaataaca cctgatgacg ttgttggcac ttacaccgaa agcgtggacc
    13561 tgccacggga agcatacctc agttatcatt tcatcaatct tcctccgaac cctgattccg
    13621 atgtattccg ataccgtgtc accggtatgg cagatgggtg cgatgtgtgt tgcagtaatg
    13681 aatatcatag tttttacgga caaaacgaca cagtaatact ctgcccggtt attgcctacg
    13741 atagcctcat tgtggaattc tatacgtttc tgaacggagt ttcgcatcaa tatacggatc
    13801 agattatcgg ggtgccttcc gacacggttg aaaaaaccat cagatactaa aatgaaccta
    13861 aaacttataa ttcaaaccgt ttttgtcagt ttgtttttca tgtcgcagtc acaaaaagta
    13921 ttcaatcaga atcgaactcc caatataatt ccaattaagg ctggctgtaa ttgttatctg
    13981 atttgctcca acgacggagc ggtacttatc gatgccggct cgcatcataa attacaagcc
    14041 tttgataaag tccttgctca aaacggtttg acatgggata caataaaata cattataatc
    14101 acacatactc attacgacca tgtggctaac cttgcggaaa taaaaaaacg cagcggagct
    14161 aaagtcatca tacacaaaaa cgagtcttcc ttcatagagt ccggcattac accacttccg
    14221 gaaggaatga ccttcactgg aaaaattggc atttgctata cccgtcatct tggcaaacac
    14281 aatgttaaag tttgtccggc agcagcagaa atttcggtag aagataatta tacaatccca
    14341 ggatttggct atagaataat ttataacccg gggcacagca gtggttcaat ttcgcttatt
    14401 ttgaacgata acatttgttt ttgtggtgat gcggcttttg ctgttttggg aaataatctg
    14461 atgcctatta ccggagaatg caaaacgggg attgttgaat cgtggataaa attacttgat
    14521 acgggatgca caattttcta ccctggtcat ggtaaaccat ttacctttaa agaactgcag
    14581 aaatctctgc aaaaatacaa aaaactttta tcgcagtgag ccacccacgg gatttgaacc
    14641 cgcgacctgc tgtttacgaa acagctgctc taccgctgag ctaaggtggc ttatatagga
    14701 gggcaaaatt agtttttttt acagaaatcc caaaacatta tggtttcgga aataatttct
    14761 gtaattcggg ggttaacgat gggtaatact tctgacacag gtttgcgtat ttggaggctt
    14821 cctcgtaacg tccgtttttt gctgcaattt gagccagata taaatatgca aaacccaggt
    14881 ctttcgattc agtaagccag taagttaaaa tttcgctgtc gtaacggctc atattttcga
    14941 cgtttccaac ctctgacaga aatctaccaa aaactttaat atttactgta tcgttcttcc
    15001 ttatggcttc ctgataacca acaaaaacag cttcctcgta gtagtactcc agcgatttaa
    15061 tcagctcagc cgatttctct atcataacta ttgccaggct atcgttatcc agttttgaag
    15121 caaacagtcc tgccttgcga tagacatacg gtgaagtgat acctgtttca accgctttat
    15181 aataataata aaaagcgctg tcgggccggt tcattctttc gtacaaatcg cccatattct
    15241 gataaaaaat ctcaaggcgg taatcttgtt tcagtttgat acgcaatttt tcgaaactcg
    15301 acaaagcctc gctgtactgc ccattataag tgaatgctat tccaagattt ccaagagctt
    15361 catagttgga agtatcaacc atgaatgcct gctgatagtt tgatattgca ccaagcatgt
    15421 ttcctttata catttctacg ttgcccagct ccatggaatg attcgattct gtacgccgcc
    15481 catcaacaga tatgcttttc agccattccg gctgcaacag tgtgataatg ccaaatacca
    15541 gaatggcaat ccaaatgcct gaaataataa agaaaatcgc cttatcgact ttcgatacct
    15601 ttgccggctg aatatttctt ttcattttca ttcctgatac agtattaaca gcaccttttt
    15661 atttttactg agcaactcgt tatatttcag gcaagcctgc ttgttaggca acttgataat
    15721 ctgatataaa ttggagtgtg taaaattcgg tctgaactcg aataatcgct ggtcgttgaa
    15781 gcccttgccc cctgtatttc cttttccggt tcctattatt aatatatcag gatttatatc
    15841 atcgatcttc aatttatctc tgccaaggaa agtttctttt ttatcaacaa cacggtaccc
    15901 tccatcagga aaaatcatta catcaaaata ataaaaaggc aagcccttat acacgattac
    15961 attttcagaa acatatttca catatgcatt tcccgaaaaa ttggcgtaaa ccagccagat
    16021 tacaacaatc ccgaaagtgc ccctgataat ccacttcagt aatcgggtat tccccatgtg
    16081 aagaataagc aggaaaaaaa atacagccaa cagagaaata agaaaaacct gcggccataa
    16141 actcggatac gagagtaccg atgaaatata aataaaaatc agcgacacac cagcctgaat
    16201 gaacagggca gatgaaggcg atgtaatata gtccttaatt ttctgatgac ggtaagtaaa
    16261 tggtttaata tgcttacatt cgagaagtac aaactgtata aaaaactgac ctattaaagc
    16321 cccccacata tctttgccaa catcagaaag gtcaaaaacc ctgttactta tatacacctg
    16381 aattatctcg tcgaataacg aaataataaa tgccattaga aaagtccgcc agattatttt
    16441 tgcaggagaa aaattcttga gaaaaaattt acggtaagca acataagaaa atattccata
    16501 ggcaatgtaa tgccagttat gctgcaaatc gtaaaaatta tgattatagt agtaatcccc
    16561 gattttttga cctatcaggg ttaatgccac catcagaaaa agcccgagaa ttcttgtttt
    16621 cgtaagttct ttgcgaacgc gaataaaaac aaatattaat cccagtatag ctataaataa
    16681 taaataagtg atttttacat cataaacagt aaaatgagca ttcgagagct tcccgattgc
    16741 atcctgcaag aagttcatca gcatgaggaa tggcgttaca atcagcaaca gcgagtataa
    16801 cgtcagattc agatttgccg agaatttgaa tttcattggt ttttcattgg actttgcaaa
    16861 gtataaaaac taattggttg aaacaaattt tcaagcctgt tttttaatag attttatgca
    16921 aaaaagtcta taagtgatgt tataaacatc aataaaacga agggtttaca gctgttttcc
    16981 ggataaaaaa acgagggttg ctccaaaacc aaattcctga aatgaagcat cctgaaaatt
    17041 gagatggtac tgagttttga gtgactccct tactttatcg cgtaatgtac cattcccgat
    17101 cccgtgaata aacactactt tctgagcttt ttcaaacatg gcatcagtga ggcttttatg
    17161 gaaatgctgc atctgataat cgagtatctc tgcattcgaa agacctttgc aatcgtcgag
    17221 aatggcgttt atatgcaggt caacctcaat ggttttgggt ttttcgcggg cagcatattt
    17281 ctcagctttg ttttcgggtg gtgcttcctt ttcactgagc aggtcggcaa gtttatgaat
    17341 tttttcgaca gtggcagcat cgggctcctg ttcattaatt atgtcccaca acaatgcctt
    17401 ggtatcgaaa taggaattgg cagcgtaatt gcccgatgta aagaaacaca caggatccgg
    17461 tttataatgc ctttcgatgg gtgagcgctg cagcattccg aatttaccgc aaaaaataag
    17521 ctggacagtt atttttttta attggttcaa tccctcgcga ttaaacaaaa acaagtcctc
    17581 gataaaaccg gggtcggcat tcccttttgc aattgttctt attccggtat catcttgctt
    17641 cagtacctga aaaagcaatt catacccact gtcgttcaca atatgcatca ataagtctcc
    17701 gtttaccgga tcgttgggca gcaccggggt aaatgccaaa ctgattctca gtttttcatt
    17761 aaggttatgt tcaacagtgt ttatctctga tgcagttttt tttacgtcag cggctttacc
    17821 aattgtattg gttttactgc cggtgtgcgg tgttacaact ctttcagttg ctatttcaga
    17881 tgctgttttc gtattttttt ttatgaggat cagctggccg gggtgcacag ggtattcaaa
    17941 atcatcgtca gtgcgcacca gcaccatgcc atcatataaa ttcctgacaa ccacggcaga
    18001 gccaatttcg tcgaggaagc gcacttcatc gcccggttca aatatatttt gcttcataat
    18061 attatattgt atcgatttaa aaaaaatgaa aaaacaatca aatgattaca tttgcgcaaa
    18121 tttcgcaata aatgggcttg ttgccaaata ttaatatgag tgaattcgga tacaatttac
    18181 ccgatgatcg catagcaaaa taccccgcat cagagcgcga ttcgtcaaaa ctacttgtgt
    18241 atcgcgatgc cgaaattcag gaatacctgt tttgtaatct tcccgaaatt ctgcccgaaa
    18301 acggtctgat actattcaat aacacaaaag taattcatgc gcgcctgcag tttcgaaagg
    18361 aaacgggtgc tgccatcgaa atattctgcc ttgagcccac tactccgtcc gattaccaga
    18421 tctcgctgtc agcgcaacaa tcatgcacat ggaaatgttt gataggaaat aaaaaaaagt
    18481 ggaaaaaaga tgctttaaaa aagctattca gaattaataa ccgtgatatt accttatctg
    18541 ctgaaatcgt atcggataca aacaacgaaa gcctgatacg tttctcctgg gatactacgg
    18601 attgcagctt tgccgaaatt cttgaacatg aaggcgaaat tcctttgcca ccctatctga
    18661 accgcaatgc agagccttct gatactatca ggtaccagac agtatattca cgtttcgatg
    18721 gatcggtggc tgcccctact gccggtttgc attttaccga taccgttctc gcggaaattt
    18781 cccgaaaaaa tataacctgc tccgaactta cactccatgt gggtgccggc acttttaaac
    18841 cggtatcgga aagcaatgtt gagaaccata cgatgcatcg cgaacatttt agcgtaagcg
    18901 ttgaaacact gaatcaaatc aggaacaacc tcgggaatat caccattaca ggaactacaa
    18961 gcttacgtgc agtggagagc ttgtattggc ttgcgggcag aatacagcgg ggtgaaactg
    19021 cagcgcatct cagtcagtgg gaaccttata cctttcaaaa cgaaattagc gctgcagatg
    19081 ctattgatat actattggaa tattgccaga aacaaaacat tgctgccttt tctgcctcta
    19141 cctcagtgat gatactgccg gggtaccagt ttcggtttgc agaccgcctc attaccaatt
    19201 ttcaccagcc taaaagcacc ctgcttttat tggtggcagc atttattggc aatgactgga
    19261 aaaaagtata tgatgttgca ttgaaccaca atttccgttt cctgagttat ggcgacagtt
    19321 cgctgctgtt taaggcgaat aactggcttt gaaaaccagt tctaataaat ctaaaaatca
    19381 ggttttgttt gtaaaaacac attcacacgc cccaggatta ttcctgatat attccacaat
    19441 actttggtat tccgcatcat tgcggataat acggtcgtcg taatcggttt gccaattttt
    19501 ccgtcccgcc gtatttcggg caatattgat ttgtttcgat gtttgcattt tgaatttccc
    19561 catgattttc gttattaaca tattcacatc tcgacctcgt ttgcaacgag gtcgcattta
    19621 tgctaagcat ttataatgcg ttaattgcag tgcattagaa atgcaccaaa taattaagtc
    19681 ctcgttgcaa actaggacta gactaggaac tgaagaggtg ccaaatggtt tatgggtcgg
    19741 agaataaatc ctgttttgaa actaatataa tatgtttata tttgcaattc ttatcataca
    19801 tcaataagaa acgcaaaata aaacttgtag caatggaaca aagaaaatat aattatattg
    19861 aggaatacct taacaaaata agggctgccg gaggctttac ttttacctat tccgaattgt
    19921 tagggacttt taatgttacc aaagaagcaa tagacctttc gttacagcgc ttaaaagcgc
    19981 gaaatagtat tgcccagatt aaaaaggggt tctatgttat acttactccg gaatatgctc
    20041 aaaaagggat gataccttct gcactatttg ttgatgacct catgaaatct cttaataaga
    20101 agtactatgt tggtttactt tcggcagcag cattacatgg tgcgtcacat caacaaccat
    20161 tggaatactt tgttattact caggctcctg ccatacgcga cattaatgct tcaaatatgc
    20221 gggtccgctt ttttgtaaaa aaggattggc atccacaatc agtggttcaa ataaaatctg
    20281 attcgggata catgaatgtt tcttcacctg agcttacagc tctagacttg tttttttatt
    20341 cccattacat ttcgacaaat agggcatata cagtactgca ggaactgcaa gaagcaatga
    20401 aaccgtcgaa gttaaaaagc acagcactat tgtttccaca aacggcatct attcagcgtt
    20461 tagggtatat acttgaacaa attcaaggtt gtgataaact ggctgattca ttaaaaaagg
    20521 tattaaatac acggaaatcg tattacatac ccctatcctc aggcaaatta aaaaaaggaa
    20581 gtgtaaatac ccgttggaaa ataattataa atactgaatt ggatggcgac ctatgatacc
    20641 aaaacgatat attgaggaat ggaaacaaac tgttccatgg attgataaca atcaggttga
    20701 acaggatttg attatttctc gcgcgttagt ggcaatttat tccgacagct ttttaatgga
    20761 aaatttggca tttcgtggag gtacggcatt gcacaaatta tatgcaaatc ccgcttcccg
    20821 atattccgaa gatattgatt tggtacagat aaagccaggc cctattggag atgtgataac
    20881 tcaacttcgt aaagctctct tgtttatcga aggatttaaa ccaacaatag atcgaggtgt
    20941 tgcaatgact acattacgat ttcgttacgt ttcagagagt tttactaacg taaacatgaa
    21001 actaaaagtt gaaatcaatt gcagagaaca tattgcagtg tatggcttaa aacatttcaa
    21061 ctattcagtg tcaaattcat ggtttacggg ttcggctcaa cttattacat ttgaaccaga
    21121 agaattgctt gccacaaagc ttagagcatt ataccagcga aagaaaggcc gagacctttt
    21181 tgatttatgg tacctgctta ctaaactact ccccattcct tagaccactt ttttgtattc
    21241 ttaattgaaa tcttta
//
LOCUS       DAOOQP010000045        21061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3075_length_21061_cov_5.458440, whole genome shotgun sequence.
ACCESSION   DAOOQP010000045 DAOOQP010000000
VERSION     DAOOQP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_21061_cov_5.458440"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLK75_06415"
     CDS             complement(<1..925)
                     /locus_tag="PLK75_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY38916.1"
                     /translation="MKTRYIILAVFYIMFFLVTSANAQKKSETFDKNKKEKLWGIPRM
                     GYKELPKKARKHFEGIIFVQGGTFTMGKSSQDMSFTDKDSTLLVGYFPRRVTVSSFFI
                     SDHEVTNAEYREFTRWVTIRNAMDILAIKYPERRLPNGHYNESIPFDWNDTVLEAFMF
                     FPEHERYYRRKEMDVSKLTYPYIRETVNDSLFQLYSDSAYRTMPQKLLYMSYEEVKVS
                     VYPDTLCWVNDFAYSYNEPMSQMYFWHPAYDNYPVVGVSWHQAMAYCQWRSDRLNEDI
                     LIAEKILKQRSFYFTTDEFLMDTANYDYMFLL"
     gene            complement(1120..1503)
                     /locus_tag="PLK75_06420"
     CDS             complement(1120..1503)
                     /locus_tag="PLK75_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOY38917.1"
                     /translation="MKKIAVPVAADGTIDSHFGHCHSYSVFTADENNNIVKVENIPSP
                     QGCGCKSNIAAVLAEQGVSVMLAGGIGDGAINVLNYWGIDVVRGCIGNAENAVKQWLS
                     GDIHDSGSSCAQHQHHNESGHQCGH"
     gene            complement(1511..1906)
                     /locus_tag="PLK75_06425"
     CDS             complement(1511..1906)
                     /locus_tag="PLK75_06425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY38918.1"
                     /translation="MDMEITFDGGKVVTAHFNGHDIKTDQPSFAGGLNSAPTPFELFL
                     ASLGTCAGIYVKFFCDSRNLPTDGIRIIQHMEFDNQSGLPLNFEFEIQVPPDFPEKYH
                     SALVSSAEFCKVKKTIENPPTFKAYTTVK"
     gene            complement(1923..2330)
                     /locus_tag="PLK75_06430"
     CDS             complement(1923..2330)
                     /locus_tag="PLK75_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOY38919.1"
                     /translation="MPRPCKCRKISSPPLMEGFEPFGITVVPVTAVNMSLEEFESFRL
                     VNYHKKSQDEAAVLMQVSRPTLTRIYNRALSHIAVAFAEGRQLLIDGGNFKLENDWYR
                     CRKCCRLIQGKSNHFPCATCDCYSDEELVLINK"
     gene            complement(2438..3151)
                     /locus_tag="PLK75_06435"
     CDS             complement(2438..3151)
                     /locus_tag="PLK75_06435"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOY38920.1"
                     /translation="MKWGKWIGGGLGWVAGGPIGLLVGFIVGTIYDSATTGRKSNTTT
                     QPGDFAVALVVVTAMVMKADNVIKKSELDYVKNFFIRNFGETKARQALLMLRDVLKKE
                     IPAREVCEQLRINLDYSSKLQMLHYMFGIAASDGEIHPNEVAVIRQMAQWMDIKQSDY
                     NSIQAMFVGDEDAAYKILEIEKTATDEEVKKAYRAMAIKYHPDKVAHLGDDVQRAAKE
                     KFQKLQEAYETVKKQRGMK"
     gene            complement(3148..3735)
                     /gene="coaE"
                     /locus_tag="PLK75_06440"
     CDS             complement(3148..3735)
                     /gene="coaE"
                     /locus_tag="PLK75_06440"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOY38921.1"
                     /translation="MEKHHVVITGGIASGKSVAAKVFEILGASVYNADANAKRLMQSD
                     PDIRQMLTDRFGQQVYAGDELNTDFVSSIIFESTENRRFVETIVHPAVLNDYLSWRET
                     ITCRVSVLELALIHSLPLREISDTIIVIDSSDELRIDRLMQRNGYTRKEALQRIASQP
                     LRAEYVKIADIVIDNNGVDFFITKLIDSYHQLPLQ"
     gene            complement(3736..4650)
                     /locus_tag="PLK75_06445"
     CDS             complement(3736..4650)
                     /locus_tag="PLK75_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38922.1"
                     /translation="MFLVFVAISTVLWFLNALEKEYSTTIDFVVKYENIPYNSMPAEG
                     SPEKVSVSIRGHGYNLLKYKLSLSNMPVVIDFSEYKLSRSQNDKNNYFFRMANLSELF
                     MKRFDNDIYFESISPDTLFFTSFKTEKKRVAVKPDLHYKLENQHKIAGSITLSPDSVT
                     IYGSPVILKTIDSVLTESINLGVISSSMKKKVAIKTYDKVKIEPKSVNLEIVVEKCTE
                     KTLSIPVNPINFPDTVNALFIPGSVDVTFNVSVENYDKIEASDFRLVADYAKSVIGVV
                     PVSLAKTPASVSDVRLKKSEVHFIIEKK"
     gene            complement(4734..5054)
                     /gene="yajC"
                     /locus_tag="PLK75_06450"
     CDS             complement(4734..5054)
                     /gene="yajC"
                     /locus_tag="PLK75_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOY38923.1"
                     /translation="MMSIILFASNANGQKGAGWEQLIFLVLIIVVFYFFMIRPQMKRQ
                     KEMRKFREGLKKGDKVITTGGIHGRIIEVEEQTVLVEVDSNVKIRIDKAAVISSPSDI
                     PQSK"
     gene            complement(5101..5565)
                     /locus_tag="PLK75_06455"
     CDS             complement(5101..5565)
                     /locus_tag="PLK75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY38924.1"
                     /translation="MLFRVIKYLFLFLAAQLLFACVHKQGDTSNVEDYNIQNPNTANG
                     KTDLSKLPVFKFTEEEYNFGTLIQGEKVSHSFVFTNTGGSNLVIKEVKASCGCTTPKW
                     TKDPVKPGEKGVIEVIFDSAGREGEQSKTIKVEANTQPNIVELRIRCEVVAD"
     gene            complement(5599..6549)
                     /gene="nusB"
                     /locus_tag="PLK75_06460"
     CDS             complement(5599..6549)
                     /gene="nusB"
                     /locus_tag="PLK75_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOY38925.1"
                     /translation="MISRRLLRVKVMQVYYAQTEKEINKLENALNELERSAGASLELY
                     FFLNCLLLCFRDLEEERLEIASNKLVPTEEDLHPNTLFADNLVLKKLTANRQIQHYYK
                     TDVFNLHENKEILKRLWSRIRESAYFRQYLEADDKGIKATKKIVTDILELEISVNEEI
                     EQLIEEKNIYWNDDLEFIISILIRNIRAIKVADDYQNEKVELYQNSDDRQFGVELLKS
                     VLLKRDTYDAIISRHILNWELERVAIIDRLLIHMAMAEFFDMPSIPARVTINEYIELA
                     KFYSTEKSSMFINGILEKVYVDQKKQGNIHKIGRGLVGDI"
     gene            6802..7152
                     /locus_tag="PLK75_06465"
     CDS             6802..7152
                     /locus_tag="PLK75_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOY38926.1"
                     /translation="MQDYENKGKDEIYSRAIRAGKRTYFFDVKETKNGDYYLTVTESK
                     KVFDRDGNSHFEKHKIFLYREDFEKFMEALRESVDFIFQNKPFTEEKKYEADATGNEF
                     SDVDFDDLADEKSS"
     gene            complement(7198..7653)
                     /locus_tag="PLK75_06470"
     CDS             complement(7198..7653)
                     /locus_tag="PLK75_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOY38927.1"
                     /translation="MNSTLRYEHILTQLQQLFASVTNPIARMATVCALLKNKMGSGYY
                     WTGYYLLDSDELIVGPYQGTLACMKLKKHTGVCWKAVLTCETIVVADVHAFSGHIACD
                     PKSKSEIAVPLYDAKNHIIGVLDVDSSILNYFNENDKDYLEKINRLIYS"
     gene            7736..8269
                     /locus_tag="PLK75_06475"
     CDS             7736..8269
                     /locus_tag="PLK75_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOY38928.1"
                     /translation="MKLILLSLLLSASVLSFAQSSSFYSLKANDINGNEFDFASLEGK
                     KVLIVNTASKCGFTPQYKELEELHQKYGKELVIIGFPANNFMSQEPGTNEEIAEFCQK
                     NYGVSFLMMEKISVKGNDMHPVYQWLTQKDKNGKEDSSVSWNFQKYMIDETGKLYGYA
                     KPSVKPFDDQIIDFIKK"
     gene            complement(8274..9410)
                     /locus_tag="PLK75_06480"
     CDS             complement(8274..9410)
                     /locus_tag="PLK75_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY38929.1"
                     /translation="MTKPNFDDLRPYYDHEVQSAMQRIVADPLFAGVAGFLYPDKSVA
                     EVAAMISSINSSFDFQKKVMYHAITRIAAKTMTKFTCNGLQFLDKNKAYLFISNHRDI
                     LLDSALLQIALSDNNLNTSEITFGSNLMQSSFVVDIGKSNKMFKVVRGGTPRDFYRNS
                     LHLSQYIRHTILEKNESIWIAHRNGRTKDGNDLTDQAVLKMFSMSGDKDLIDNLLELN
                     ITPMVVSYQWETCDVSKVNELYISKTSKYEKKPGEDLQSILNGITQFKGNVHIEVTDP
                     VTREEIVGNGSGEKPEIFNNLTSAINKRIHLGYALWDSNYIAYDIENNSRVYEGDKYS
                     ASQKEFFIDLMNKRISLINGNKEQIQAIYLQLYSNPLKNALMYK"
     gene            complement(9410..10747)
                     /locus_tag="PLK75_06485"
     CDS             complement(9410..10747)
                     /locus_tag="PLK75_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY38930.1"
                     /translation="MEENYKTRNLNPDIEYQDPLVIKQFQEKKLRETLFYLQQKSSYY
                     KRLFHSENIDISAIKQLEDLKYIPLTDKTCLQQYNEDFLCVEREKIVDYITTSGTLGD
                     PVTFAMTDNDLDRLAYNEAISFSCAGGHQGEIYQLMTTIDKRFMAGLAYFLGIRRMGA
                     SIIRVGNGIPELQWDTINRIRPDAIVVVPSFIMKIIKYAEENGIDYRKSSIKKAVCIG
                     ENLREQDFTLNLLGRKIKEKWDIELYSTYASTEMATSFTECSCGCGGHHHPELIIAEL
                     VDENGCIVPEGEAGELTITTIGVEGMPLLRFRTGDIARFFTEACSCGRKTMRISPIIG
                     RRKQMIKYKGTTLYPAAIFDVLDNVEYIDNYYVVVDTDENGNDDIVIYIGCRAEHQSA
                     GENIQKELKDRFRAKLRVAPNVTLADPEEIKKVNANDVNRKPVKFIDKRNKYI"
     gene            complement(10766..11623)
                     /locus_tag="PLK75_06490"
     CDS             complement(10766..11623)
                     /locus_tag="PLK75_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY38931.1"
                     /translation="MIVKNFVFIWIAMMIENTLFKGKEEVFLWKDVGNPNQEKVKLYV
                     CEPEANIKNGTCVIICPGGSYHHLGMTHEGFYVADWLNSQGITAFVLKYRVSQFGWHH
                     PAMIEDLQKAIQYVRQNASKYGVNPESVGVMGFSAGGHLVGTASLYYDENYIGNTESD
                     VSLRPDFTVMVYPVVSMQDSIAHQRSKKNLLGKAKDKDLEDKMSLEANVHQGIPPVLL
                     IHAKDDDVVDYRNSFYLNENMKKAGVDVEYLLYENGGHGFGAGKTKKYTPPWQADCLE
                     WLHKKNILK"
     gene            complement(11860..12234)
                     /locus_tag="PLK75_06495"
     CDS             complement(11860..12234)
                     /locus_tag="PLK75_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38932.1"
                     /translation="MKISNDHIDIIYDFKGLWDLPSRCGLKIVNKKSHSVIIVTELYR
                     DNPGTSVTNVAASLAMQICDEFSLNPGKLVYIECNPEMKSKLSFYSEKNYLVQFTCDG
                     SELRDPNYKILSPEELDNLVTE"
     gene            complement(12242..13522)
                     /locus_tag="PLK75_06500"
     CDS             complement(12242..13522)
                     /locus_tag="PLK75_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY38933.1"
                     /translation="MQIKFKLIYPKWKKLEGQTTFNLPPHGPVVMAAALPDYVDVSFT
                     DENVEDIDFDEVCDFVGISMMLSTQVKRGWAIAHEFRKRGKKVIFGGISTMLHAEETM
                     LHADSVFLGECEGRLEEVFEDFKNNNLKKLYNYLVQLPPIDLVGPARREILKREMYNH
                     KGVQMVDLFHASRGCRFSCYPCAVSYLGGRQFRPRPIDRVLEELETIDNNRLFIVDNS
                     LAQNTQWEIDLFRNMIPMKKKWISHTIEDDPKVLDLAAQAGAWYVYQAVFDTSDYIKE
                     RIKRYHDYGIGVEGTILLGLDNQSEDDIKRLIDFLLEIDLDLAEFTVLTPFPHTKVYD
                     DLKREGRIFDHDWDHYNADTVVYKPAQMSPERLHELYRYAWEAFYKDESQEQKMFKLF
                     SKIVLREMNDGTFKPRNRNLATKSFGKEVIRNIS"
     gene            complement(13540..15048)
                     /locus_tag="PLK75_06505"
     CDS             complement(13540..15048)
                     /locus_tag="PLK75_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOY38934.1"
                     /translation="MGKNVIIIGGGLGGLVCACLLSKEGYKVTILEKHSIVGGGLHCF
                     KKHGVLFETGIHYISGFQEGGVLRKLFNYLNITDKLDIKPLNKDGFDVMHVGSDDRKY
                     YMGVGKENYIRLLSESFPEETENIIAYVNGIYGLADAIPLFNLRFKTESLGYFDDSFL
                     ISVGDYISSFTTNKKLQMVLAWDNTLYAGAYDVTPIYIHALVTKFCIEGASRFNGGSQ
                     QLADAMVEIIQNSGGNVILNAEVCKIDVENKLIEAIHTTDKKTYSADHYISSIHPAIL
                     MDMIEPEKVQASYRNRLQNIENSYSAFTVYAILKPKSFPFLNYNYYYFDNYDMVWHPT
                     VYDYESFPIGFMLITPSIQNQGEYAEKVIINCIMKFDDFKKWEATQQGKRGQDYYDIK
                     QQYQQKILDKVNKVFPGFYDSIEHVFSSTPLTLRDYLGSKDGSLYGYMKDCRNIVKSQ
                     LVPRTKIDNLLLTGQNLNLHGIVGVPLSAIVTAGSLVGVDNIINKIVEANKK"
     gene            complement(15048..16547)
                     /locus_tag="PLK75_06510"
     CDS             complement(15048..16547)
                     /locus_tag="PLK75_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOY38935.1"
                     /translation="MAKYDVVIIGSGLGGLLCGYILVKNGFKVLILEKNRQIGGCLQT
                     FNSGGVKFDTGMHYIGSMDPGQVMHRFFRYFSLLEDVKLQKLDEDGYDVISYKDSIYK
                     YAMGFGNFVDVLSESFPAERQHLKTYVDRIQEIALESPLYNLMEIEQNVFLKIESVKS
                     SINEFIDSITKNTTLRNVLVGNLPLYAGKKDITPLYIHTLINNFYIQSAYRIVGGSDC
                     IASSLATSIRKSGGEIMTQSEVVKFNCDSEKMTSVLLKNGEIIEGKRFISNIHPQATI
                     DKIDTSLIRNATRVRISNLTNSISNFTVYITFRKNRVKYMNSNFYYYAGNDVWECENY
                     TSENWPLNYLYMHQAPVDDNASGYASSAVLIGYMRYDEVEQWESTKVNRRGVEYLEFK
                     QRKAEKLLSSLEKSFPGIMSEIESYSTSSPLTYRDYTATKEGSMYGVLRDKNFPTQTL
                     VSQRTKIPNLYMTGQNINSHGILGVTIGAIVTCSEFMGINTIINDIKNC"
     gene            complement(16547..20227)
                     /locus_tag="PLK75_06515"
     CDS             complement(16547..20227)
                     /locus_tag="PLK75_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY38936.1"
                     /translation="MSRIVLSIYHFFSRRKWLVFTISALLLLLFGYFASRLTLEEDIS
                     GFMPGEKNNEKINFLYKNIGVSERIIVKISSQGKKNQCDSLLEAADYFANHLKYVTDG
                     KNLVHDVFYKIDQQLIFDVAGFITQNLPYYLSESDYERIDRLINRDSISKILANNKKL
                     LISPAGMVLKQQIISDPLHLATPVLSRLNEFQISSDYIIYNNYIFSNDSTNILLFVTA
                     SNPVSKTAENAVLVDYLDESVDSVRKHFNADVRYFGAAPVGVSNAKQIKNDSYLAILI
                     SVVLILGLLLWFFRSVKSIVLIAVPVLFGALMALAALYFLKGTISAIAIGAGSVIFGI
                     AINYSLHYLIHAKYAHNQGEVLRDISSPMITGSITTVGAFLSLLFISADSMRDFGLFA
                     AFSLAGTLLFVLIFLPHFLKNQPNLNVEKTGVIDRISGYRIDRNRWIITVVVLLTFVF
                     FYFSFDVNFETEMNKINYMTPEQKESFEEMSAYTTLGEKSVYFVSDAATLDVALQQYE
                     SKKDVIDSLREAGTIQSISGIGQFLPSSQMQKAKIQKWNQFWESRSVTFKDIFVEEAV
                     KQGFKKESFDGFLNALDSTYTVQPSAYFSIITENFLKDYLIVTGERAMVVSVINTGKD
                     NTAEVSRKLSGSNCFVFDSGSVAQSLVDLLSDDFNRVLYICSFLVLLFLTISFGRLEL
                     SVIAFLPMLISWIWILGIMSVFGVGFNIVNIILATFIFGLGDDYTIFMMEGMMSEYAS
                     RRKLLSSYKTAVVLSAVTMFVGIGTLVFAKHPAMKSLGNVTIIGMISVVLISFIIPPI
                     FYHAITYKNGKKRLIPVTFVNFFATVYSFCAFLLGTLVLTCIGFFLLGISRKTDKNKL
                     RYHKAMQYVTDLVTRKIPFVKREIINEYNEDFSQPGVIICNHQAHIDLMYIMMLSPKI
                     VILTNEWVWNSPFYGRMIRYADYYPVATGIENSIARLGQMVEKGYSIMVFPEGTRSED
                     CSILRFHRGAFYLAEKLNLDIIPVIVHGLGHALPKKEMLLRKGKATIKILQRIKPDDK
                     MYGDNYSERARNIRKMYIEEYGNMVDAIETPSYFSNLVIHNYVYKGAFIERSARRLMR
                     SYSNFEALITSLPDTGRVCIAECGFGTVSLMISLVKKNLQVYATDNDRDKLDIAGNCV
                     SNPPNLHYSEPADEDIDYSEFDLIAGVEPSEQTIRKCLLANRDFIFLVAEMNSGARLF
                     VDMGCVVSEGNKKGIWIVKNRK"
     gene            complement(20304..>21061)
                     /locus_tag="PLK75_06520"
     CDS             complement(20304..>21061)
                     /locus_tag="PLK75_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HOY38937.1"
                     /translation="GAITLGARYKIFRISYSFNITTDQDYNLCYGPTRFNSFEMGIKT
                     RFLWLEAYFSRCKGFYYSDQSILFPKFSTDSIYPQNNGLSSFEVGLRAKLIFDHDFSM
                     EAAFDYSEIQEKSAGSFFYTLNPEISNIKSDSLPIIPPIYTPYYSGLNDFRKATFLSF
                     SNCLGYGYSLVLGPVNFSNAIAAGPNLQLSFNHGIKVKLPFDLYFKSSLSVNTKNFYT
                     GAMVSLDVNNHVFGQNNIRKQFLVFSFRMGFRF"
BASE COUNT         6273 a         4453 c         3808 g         6527 t
ORIGIN      
        1 acaataaaaa catataatca taattggctg tgtccatcaa aaattcatct gttgtaaaat
       61 aaaacgatcg ttgttttaat attttttcgg caataagtat gtcttcgttc aaacggtcgc
      121 tgcgccattg gcagtatgcc atagcctgat gccagcttac acccacaacc gggtaattgt
      181 cgtaggcagg atgccagaaa tacatttgcg acatgggttc gttgtaagaa taggcaaaat
      241 cgtttaccca acaaagcgta tcgggatata cactaacttt tacttcttca taactcatat
      301 aaagcagttt ctggggcatg gttctgtaag cactatctga atatagctga aacagactat
      361 cgtttaccgt ttccctgatg taaggataag ttagcttaga aacatccatt tctttgcggc
      421 gataataacg ctcgtgttcg gggaaaaaca taaaggcctc aagcaccgtg tcgttccagt
      481 cgaacggaat ggactcgttg taatgtccgt tgggcagacg cctttccgga tattttatgg
      541 caagaatatc catggcattg cgaatggtta cccatcgggt aaactcacgg tactcggcgt
      601 tggttacttc gtggtcgctt ataaaaaatg aacttacggt aaccctcctt ggaaagtacc
      661 caaccagcaa agttgaatcc ttatccgtaa atgacatatc ctgagatgat ttccccatgg
      721 taaaggttcc tccttgcaca aatataatcc cttcaaagtg cttacgggct tttttaggga
      781 gctctttgta acccatccgg ggaatacccc aaagcttttc ctttttgttt ttatcgaatg
      841 tttctgattt tttctgcgca ttggcactgg ttaccaaaaa gaacataata taaaaaactg
      901 ctagtataat ataccttgtt ttcatgctgt cggattttat ctcataaata tatatgaaat
      961 ataacttata agcaaatatt aagtttattt tcagttatgc tcttttaaat caatatagaa
     1021 caaatatatg aacagaaata cgccaatagc ttgttttcat accattttgc tccaattctt
     1081 aaatgagaag tataggtacg ataactaaaa tgaatatgct taatgcccgc actgatgccc
     1141 actctcgttg tgatgctgat gctgggcgca gctgcttcct gaatcgtgga tatctccgct
     1201 aagccattgt tttacggcat tttcagcgtt accgatgcaa ccacgaacaa catcaatgcc
     1261 ccagtaatta agcacgttaa tggctccatc gccgatacca ccggcaagca taacacttac
     1321 accctgctct gccaacacag ctgcaatatt cgatttgcat ccacaaccct gaggcgatgg
     1381 aatgttttca acctttacga tattgttatt ttcgtcagct gtaaacactg aataggaatg
     1441 acaatgccca aaatgagaat cgatggtgcc atcggcggca accggaactg caattttttt
     1501 cataattaaa ttatttaact gttgtatatg ctttaaatgt cggtggattt tcgatagttt
     1561 ttttcacttt gcaaaattca gccgaactta ccagagccga atgatatttt tcgggaaaat
     1621 cgggcggaac ctgtatttcg aattcgaaat tcaagggtaa tccactctgg ttgtcgaact
     1681 ccatgtgctg aatgatgcga ataccatcgg taggtagatt acgactgtcg caaaagaatt
     1741 tcacataaat acctgcacag gtacccagcg atgccagaaa tagctcaaat ggtgttggag
     1801 ccgagtttaa tccacctgca aaagaaggtt gatccgtttt aatatcatgt ccgttaaaat
     1861 gagcggtaac tactttaccg ccgtcgaaag tgatttccat atccataatt acttgattta
     1921 atttatttat taataagtac taattcttca tcgctgtagc aatcacatgt tgcacagggg
     1981 aaatggttgc tttttccttg aatcaagcgg caacatttcc ggcaacggta ccagtcgttc
     2041 tccagtttaa aattacctcc atcgataagt aattgccgtc cttcggcaaa tgcaacggct
     2101 atgtggctga gtgcacggtt atagattctt gttaacgtgg gccgagagac ctgcatcaga
     2161 actgcggctt cgtcctgcga cttcttgtgg taatttacca acctgaaact ttcgaactcc
     2221 tccaggctca tattcactgc tgttaccggt actacagtaa taccaaaagg ttcaaacccc
     2281 tccatcagtg ggggactcga aatttttcgg catttacagg ggcgtggcat agtaattttt
     2341 ttgcaaatat aatgaactta cgttcataat acaaatttga taagaaattg ttcaaaaaaa
     2401 ttaattacct aaaataacta acaaaaaacc gattgcttta cttcatcccg cgttgcttct
     2461 tcaccgtttc gtaggcttcc tgaagctttt ggaatttttc tttggcagcg cgctgcacat
     2521 cgtcgccaag gtgggctacc ttgtcagggt gatatttgat tgccatggca cgataggctt
     2581 ttttcacttc ttcgtcggtg gctgtttttt cgatttcgag aattttataa gcagcatctt
     2641 cgtcgccaac aaacattgcc tgaatgctgt tgtagtccga ttgcttgatg tccatccact
     2701 gagccatttg ccggattact gctacttcgt taggatgaat ttcaccgtcg gaagcagcaa
     2761 taccaaacat ataatgcagc atctgcaatt ttgaggaata gtcaaggttt atgcgaagtt
     2821 gctcgcatac ttcacgtgca ggtatttctt ttttcagcac atcgcgaagc atcaacaagg
     2881 cctgtcgggc tttggtctca ccaaaattcc tgatgaaaaa gtttttcacg taatcgagtt
     2941 ccgacttttt aattacgttg tcggctttca taaccattgc agtaaccact acaagagcaa
     3001 ctgcaaagtc gcctggctgg gtagtagtat tcgatttacg gccggttgtg gcactgtcgt
     3061 agatagtgcc gacaataaaa ccaacaagca gcccgatggg tcctcctgca acccatccca
     3121 gtccgcctcc tatccatttt ccccatttca ttgtaacggt aattggtgat acgaatcaat
     3181 taattttgta ataaagaaat ctaccccatt gttatcgata acaatatctg caattttaac
     3241 atactctgca cgtaaaggtt gcgaagctat tctttgcaaa gcttctttcc ttgtatatcc
     3301 gtttctttgc atcaaacgat caatgcgcaa ttcatcgctg ctatcaatta caattattgt
     3361 atcggatatt tcgcgaagag gaagtgaatg tattaatgca agctccagca cgcttacccg
     3421 acaggtaatc gtttcgcgcc acgaaagata atcgttcaaa acagccggat gaacgattgt
     3481 ttcaacaaat ctgcgatttt cagtgctttc gaaaatgatt gaagacacga aatcggtatt
     3541 taactcatca ccagcataca cctgctgtcc aaacctgtcc gtaagcattt gcctgatgtc
     3601 gggatcgctt tgcatcagtc gctttgcatt ggcgtcggca ttataaaccg atgccccaag
     3661 aatttcgaaa acttttgcgg caactgattt accggaagca atgcctcctg ttataacaac
     3721 gtgatgcttt tccatctact ttttttcgat aataaagtga acctcgctct ttttcagccg
     3781 gacatccgat accgaagccg gagtcttggc gaggcttacc ggaaccactc ctattacgct
     3841 tttcgcatag tcggcaacca ggcgaaagtc gctggcctca attttatcat agttttcgac
     3901 agaaacatta aaagtaacat ccacactccc cggaataaac aaagcattta cagtatcagg
     3961 aaagtttatc ggatttaccg ggattgaaag cgttttctcg gtacattttt ccaccacaat
     4021 ttcaagattc acagacttag gctcaatttt taccttatca taggttttta tcgccacctt
     4081 ttttttcatt gagctggaaa ttactccgag attaatgctc tcggtaagca ccgagtcaat
     4141 agtttttaaa ataacaggtg aaccgtaaat tgtaaccgaa tcgggactca gggtgatgct
     4201 acctgcaatt ttatgctgat tctcaagttt gtaatgcagg tcgggtttca ccgccacccg
     4261 tttcttttct gttttaaacg acgtaaaaaa aagtgtatca ggcgaaatac tttcgaaata
     4321 gatatcgtta tcgaatcgct tcataaaaag ctccgataaa ttggccatcc tgaagaaata
     4381 attgttttta tcgttctgag agcgagacaa tttgtattcg ctgaaatcga taaccacggg
     4441 catgttgctg agcgaaagct tgtacttaag taaattatac ccatgacctc tgatgcttac
     4501 cgatactttc tcggggcttc cttcagcagg catactgttg tagggaatat tctcgtattt
     4561 aacaacgaaa tcaatggttg tactgtattc cttttcgagt gcattcagga accagagcac
     4621 ggttgaaata gcaacgaaca ccaaaaacac aagaaacctt ttgtttttga tcagttttct
     4681 aaaaaaaaac agaaacttgt tttttgttac tatttgttca ttttcgggca aagctatttg
     4741 ctttgaggaa tatcggaggg gctgctgatg accgctgctt tatcgatacg gattttcaca
     4801 ttggaatcca cctccaccag cacggtttgt tcttccacct caataatacg tccgtggata
     4861 ccaccggtag taataacttt gtcgcccttt ttcagacctt ccctgaactt acgcatttct
     4921 ttctggcgct tcatctgtgg gcgaatcata aagaagtaga aaaccactat tatcagtacc
     4981 aggaagatta gctgttccca tcctgcacct ttttgaccgt ttgcattact tgcaaataaa
     5041 ataattgaca tcatagaatt tggttttatt taattattaa cacttattat ttttaaagaa
     5101 ctaatcagca accacttcgc aacgaatacg cagttcaaca atatttggtt gcgtattggc
     5161 ttccactttt atcgttttcg attgctcccc ttcacggcca gcactatcga aaataacttc
     5221 tatcactccc ttttcaccgg gtttcaccgg atcttttgtc catttggggg ttgtgcatcc
     5281 gcatgaagcc ttaacttcct tgataaccag gttgcttccc cctgtgttgg tgaatacaaa
     5341 actgtgcgac actttctcac cctgtatcag ggtaccgaaa ttatattctt cttcggtaaa
     5401 tttaaaaaca ggcaatttgc tcaaatcggt tttcccattg gcagtgtttg ggttctgaat
     5461 gttataatct tcaacattcg aggtatcgcc ttgtttatgc acgcatgcaa acagcaattg
     5521 agctgcaaga aagagaaaca agtatttaat aaccctgaac aacattgtag ttttttttac
     5581 aaaattaaca attatccgtt aaatatcacc aaccaatccc cgcccaatct tatgaatatt
     5641 accctgtttc ttttggtcga catatacttt ttcgagaata ccattgataa acatactgct
     5701 tttctcagta ctgtaaaatt ttgccaattc gatatattca tttatggtta ccctggcagg
     5761 aattgacggc atatcgaaaa actcagccat agccatatga ataagtaatc ggtcgataat
     5821 ggcaactctt tccagctccc agttcaaaat atggcgtgat attatggcat cgtatgtatc
     5881 tcttttcagc aataccgact tcagtagttc gacaccaaat tgtcgatcgt cggaattctg
     5941 atacaattca actttttcat tttgataatc atcggcaact ttgattgctc tgatatttcg
     6001 tattaatatg ctgatgatga attccagatc gtcgttccag tatatgtttt tttcttcaat
     6061 taactgttca atttcttcgt taaccgatat ttcaagttcc agaatatcgg taactatttt
     6121 ttttgttgct tttattcctt tgtcatcggc ttcaagatat tgcctgaaat aagccgattc
     6181 acgtatccga ctccataaac gtttgagaat ttctttgttt tcgtgcaggt taaaaacatc
     6241 agttttataa taatgctgaa tctggcggtt tgctgtaagc tttttcagaa caagattatc
     6301 tgcaaacaag gtatttggat gaagatcttc ttcagtagga accaatttat ttgaggcgat
     6361 ttcgagtctt tcttcctcca aatccctgaa acaaagtaac aaacaattaa ggaaaaagta
     6421 taattcaagg cttgcgcctg cgctacgttc caattcgttc agagcatttt cgagcttatt
     6481 aatttctttt tcggtttgag cataataaac ctgcatcacc ttcacacgta acaatctgcg
     6541 actaatcatt tcaagaacat ttgttcggca aaaataaaca aataaacttt gtttcaaaga
     6601 aatcacttaa aattgaatct tattttgcct aaatagcata agaataaagg cattaaaaag
     6661 ctatttcggc gttaaaatct ggttgcaaat gaaccaaaaa agagaaacag cccatggcaa
     6721 cgcagatttt acaaaatatc cttgtttttt aaaactaatt cacttatttt cgtaagttgg
     6781 agataaaatt tagagcttat gatgcaagat tacgaaaaca agggcaagga cgaaatctac
     6841 tccagagcca taagggcggg gaaaaggacg tactttttcg atgtaaagga aaccaagaat
     6901 ggtgattatt accttacggt taccgaaagc aagaaagttt tcgacaggga tgggaactca
     6961 catttcgaga agcacaaaat tttcctctac agggaagatt tcgaaaagtt tatggaagcg
     7021 cttcgcgaaa gtgtagattt catttttcaa aacaagcctt ttaccgaaga aaaaaagtat
     7081 gaagctgatg caacagggaa tgaattcagc gatgttgatt ttgatgacct tgccgacgag
     7141 aagagttcct gaacaggatt agctgtcgga gattaataat tatgccgatt tacccaatca
     7201 gctataaatc aatctattga ttttctcgag ataatcttta tcgttttcgt taaaataatt
     7261 cagaattgaa ctgtcaacat caagcacacc gatgatatga ttttttgcat catacaaagg
     7321 tacagctatt tcgcttttcg attttgggtc gcaggctata tggcctgaaa acgcatgaac
     7381 atcggcaaca acaatcgttt cacatgtaag aacggccttc cagcaaacac cggtgtgttt
     7441 ttttaacttc atacaggcaa gggtaccctg atatggtccc acaatgagtt cgtcgctatc
     7501 gagcaaataa tagcctgtcc agtagtagcc acttcccatt ttatttttca gcagtgcaca
     7561 aacagttgcc atacgggcta tggggtttgt aacgcttgca aataattgct gcaattgtgt
     7621 taaaatgtgt tcataacgga gcgttgaatt cataaggtca aattttttga acaacttttc
     7681 tatatttgtg ttataaagat aaaataattt actttatcaa taaattacta caaaaatgaa
     7741 actcatacta ttatcgctcc ttttatcggc aagtgtttta tcctttgcac aaagcagctc
     7801 tttttacagc cttaaagcca acgacatcaa tggcaacgag ttcgattttg caagtttaga
     7861 aggtaaaaaa gttctcattg ttaacacagc ttcaaaatgt gggtttactc cccagtataa
     7921 agagttggaa gagcttcacc agaaatatgg aaaagagctt gttattattg gttttcctgc
     7981 caacaatttc atgagtcagg aaccgggtac caacgaagaa atagccgagt tctgccagaa
     8041 aaactatggc gtcagttttt tgatgatgga aaagatttcg gttaaaggaa acgatatgca
     8101 tcccgtttac caatggttga ctcaaaaaga taaaaacggc aaagaggatt cttcggtatc
     8161 atggaatttt cagaagtata tgatagatga aaccggcaag ctttatgggt atgcaaaacc
     8221 gtcggtaaaa cccttcgatg atcagataat cgattttatc aagaaatagc ccgttattta
     8281 tacattaaag cattcttaag tggattcgag tacaactgca ggtatattgc ctgtatttgt
     8341 tctttgttac cattaatcaa cgaaatgcgc ttattcatca ggtcaataaa aaactctttc
     8401 tgtgaagcag aatatttatc tccttcataa actcgcgaat tattttcgat atcgtatgca
     8461 atgtaatttg aatcccacag agcatatccc aaatgaatgc gtttgttaat agcagaagta
     8521 agattattga aaatctcggg tttttctccc gatccgttac ccactatttc ttcccgggtt
     8581 acaggatccg taacttcgat gtgtacattg cccttgaact gagtaatacc attcaagatg
     8641 ctctgtaggt cttcccccgg ttttttctca tattttgagg ttttcgatat gtaaagttca
     8701 tttactttcg aaacatcgca ggtttcccat tggtatgaaa ccaccattgg agtaatattc
     8761 aactccagca gattatcaat gaggtctttg tcgccgctca tcgaaaacat tttaagcaca
     8821 gcctgatcgg tcaaatcatt tccatctttt gtgcgtccgt tacggtgagc tatccaaatt
     8881 gattcgtttt tctccagtat tgtatgcctg atgtactgcg ataaatgcag agaattccta
     8941 tagaagtcgc gcggtgtgcc tcctctgacg accttgaaca ttttgttcga tttaccgata
     9001 tccacaacaa aactgctctg catcaagttg ctgccgaagg tgatttctga ggtattcaga
     9061 ttgttgtcgg ataaagcgat ttgtagtaaa gcagagtcga gaagaatatc ccggtgattt
     9121 gaaataaata aataggcttt gttcttatcg agaaattgca aaccattgca tgtgaacttt
     9181 gtcattgttt ttgccgcaat gcgcgttatc gcatggtaca ttaccttttt ctggaaatcg
     9241 aagcttgagt taatcgacga aatcattgct gcaacttcag caacactttt gtcggggtat
     9301 aaaaaaccag ccacgccggc aaaaagtgga tcggcaacaa ttcgttgcat agccgattga
     9361 acttcatgat cgtaataagg ccgtaggtcg tcgaaattgg gtttagtcat cagatatatt
     9421 tatttcgttt atcaataaat ttcacaggtt tacggtttac atcgtttgca tttacctttt
     9481 taatttcttc ggggtcagca agagtgacat taggtgcaac acgcagtttt gccctgaatc
     9541 tatctttcag ttctttctga atattttctc cggcactttg gtgctcggca cggcaaccaa
     9601 tataaattac aatatcatca ttgccatttt cgtcggtatc gaccacaaca tagtaattgt
     9661 cgatatattc cacattatcg agaacatcaa atattgccgc aggatacaag gtagtacctt
     9721 tatattttat catttgttta cgacgcccga taataggact tattctcata gttttacgac
     9781 cgcagctgca tgcttcggta aaaaaccgcg caatatcgcc ggtgcggaat cgcaataatg
     9841 gcataccttc tacacctatg gtagtgattg ttaactcgcc ggcttcacct tcaggaacaa
     9901 tacagccatt ttcatcaacc aattcggcga taataagctc cggatgatgg tgaccgccac
     9961 aaccacagct acattcggtg aaactggtag ccatttcggt agaagcatag gtagagtata
    10021 attcaatatc ccacttttcc ttaatttttc taccgagtaa gttcaacgta aaatcctgtt
    10081 ctctaaggtt ctctccaata caaactgctt tttttatact cgattttcgg tagtcgatac
    10141 cgttctcttc ggcgtatttg ataattttca taataaatga aggcaccaca actattgcat
    10201 ccggtcggat cctgtttatt gtatcccatt gtaattcagg tatgccgttt ccaaccctaa
    10261 tgatacttgc acccatacgc ctgataccca ggaaatatgc caggcctgcc ataaaacgct
    10321 tatcaatggt agtcatcagc tgatatattt ctccttgatg accaccggca cagctaaacg
    10381 aaatagcttc gttatatgcc agcctgtcaa ggtcattgtc agtcatagca aaagtaacag
    10441 ggtcgcccaa tgtgcctgaa gtggtgatat aatccactat tttttcgcgt tcaacgcaaa
    10501 gaaagtcttc gttgtattgc tgcaaacagg ttttatcggt aagagggata tatttcaggt
    10561 cttccaattg tttaatcgca gaaatatcaa tattttcgct gtggaacaag cgtttataat
    10621 agcttgattt ttgctgtaaa tagaacaacg tttcacgaag tttcttttct tgaaactgtt
    10681 taataacaag ggggtcttga tattcaatat caggattcag atttctggtt ttgtagttct
    10741 cttccatttc taacaaccgg cacacttatt tcaaaatgtt ttttttgtgt aaccattcta
    10801 aacaatcggc ttgccatggc ggagtatatt tttttgtttt tcctgctcca aagccatgtc
    10861 caccgttttc gtaaagaagg tattcaacat caacaccggc tttcttcata ttttcgttca
    10921 gataaaaact attgcggtaa tcaactacat catcatcttt cgcgtgaatc agcaatacag
    10981 gaggtattcc ctggtgcaca ttagcctcca gcgacatttt atcttcaagg tccttatctt
    11041 ttgcctttcc taacagattt ttcttcgacc gttggtgagc aatactatct tgcatcgata
    11101 caaccggata caccattact gtgaaatcgg gccgaagcga cacatctgat tctgtgttcc
    11161 cgatatagtt ttcatcataa tatagcgatg ctgttcccac gaggtgccca cctgctgaaa
    11221 accccatcac tcccaccgat tcagggttga caccatattt agaggcattt tgcctgacat
    11281 actggatggc tttttgcaaa tcctcaatca ttgcagggtg atgccaccca aactgcgaaa
    11341 ctctgtattt aagtacgaaa gctgtaattc cctgcgaatt gagccaatct gcaacataaa
    11401 acccctcatg agtcattcca agatgatgat aacttcctcc ggggcatatg attacgcaag
    11461 ttccgttttt aatgtttgct tccggttcgc atacatataa tttcactttt tcctgattcg
    11521 gattaccaac atctttccat aaaaacacct cctctttacc tttaaaaagg gtgttttcaa
    11581 tcatcatggc gatccaaata aaaacaaaat tcttcacaat cataacaatt caacttcgag
    11641 gttacatcag ccggcaattt ataaaaaaat aggttctact accattttag tgggtaaatt
    11701 cacaaaaagc atggctatct tgctatatat catctattta tcattgtatg cgcgtaatat
    11761 tagggaatct gttcgtaact atttgcttaa cagattgata tgtcgtaaat ttcatcttct
    11821 ttccactaaa tttgcagtag aaccaaaaaa taaataaatt tattctgtta ctaaattatc
    11881 caattcttca gggctgagta ttttatagtt ggggtccctt aattctgagc cgtcgcaggt
    11941 aaattgaacc aaatagtttt tttcgctata aaacgataac ttcgatttca tttcggggtt
    12001 acactcaata taaacaagct ttccgggatt aagcgaaaat tcgtcacata tttgcatagc
    12061 aagtgatgca gcaacattgg ttacagaagt acccggatta tcgcggtaca attcagtgac
    12121 gattatcaca gaatgagatt ttttattaac gattttaagg ccacatcgtg atggcaaatc
    12181 ccataatccc ttaaagtcat aaataatatc aatatgatcg ttacttattt tcatgcttca
    12241 attaactgat atttctgatt acttcctttc cgaacgattt tgtcgccaaa ttgcggttac
    12301 gtggtttaaa agtaccatca ttcatttcgc gcaaaacaat tttcgaaaac aatttaaaca
    12361 ttttctgctc ctgtgactca tctttgtaaa aggcttccca tgcatagcga tacaactcat
    12421 gcaagcgttc cggtgacatt tgggcaggct tataaaccac tgtatcggca ttatagtggt
    12481 cccagtcgtg gtcaaatatt ctgccttcac gctttaaatc atcatatacc ttggtgtgag
    12541 ggaatggagt aagcactgta aactcagcaa ggtcgagatc aatttcaaga agaaaatcaa
    12601 tcagtcgttt aatatcatct tcactttgat tatcaagtcc caataaaatt gttccctcca
    12661 ctcctattcc atagtcgtga taccgtttga ttctttcttt aatataatcg gaggtatcga
    12721 atactgcctg atatacatac catgcacctg cctgtgcagc caaatcaagc actttggggt
    12781 catcttcaat agtgtgacta atccattttt tcttcatggg aatcatgtta cggaacaaat
    12841 cgatctccca ctgagtgttt tgagcaagag aattgtcaac tatgaacagg cggttattat
    12901 cgattgtttc gagctcttcc agcactctat caatcggcct aggccgaaac tgacggcctc
    12961 caaggtagga tacggcgcaa ggataacaac tgaacctgca ccctctcgat gcgtgaaaaa
    13021 gatcgaccat ttgcacaccc ttatggttgt acatttctct tttcaggatt tcgcggcgtg
    13081 ccggaccaac caaatcgatt ggaggcaact gcacaagata attatagagt ttttttaagt
    13141 tgttgttttt aaagtcctcg aaaacctctt ccagcctgcc ttcacattct cccaaaaaca
    13201 ccgaatcagc atgaagcatg gtttcttcag catgtagcat agtggaaata cctccaaaaa
    13261 tcactttctt gccgcgcttc ctgaattcat gagcaatagc ccaaccacgt ttcacctggg
    13321 tgctaagcat catcgatatg cctacaaaat cgcatacttc atcaaaatcg atgtcttcga
    13381 cattttcatc ggtaaacgaa acatccacat aatcgggcaa agcagccgcc ataacaacag
    13441 gaccatgagg tggcagatta aaagttgtct gcccctctaa tttcttccac ttcgggtaaa
    13501 ttaatttgaa tttaatctgc attgtttgaa atgaatatgt tattttttat ttgcttcaac
    13561 tattttatta ataatattat cgactcccac caaagatcct gcagttacaa tagcactaag
    13621 tggcacaccg acaattccgt gcaaatttaa gttttgtccg gttaataaca aattatcgat
    13681 tttagttctt ggcaccaatt gcgatttcac tatgttccgg caatctttca tatatccgta
    13741 aagcgaaccg tcttttgatc ccagataatc gcgtagtgta agcggtgttg agctgaaaac
    13801 atgctcaatc gaatcgtaaa aacccgggaa aaccttattt accttatcta aaattttttg
    13861 ttgatactgt tgtttaatat cgtaataatc ctgccccctc ttgccctgct gtgtagcctc
    13921 ccattttttg aaatcatcaa atttcataat acagttaata atgacttttt cggcatactc
    13981 accttggttt tgaattgacg gagtaataag cataaaacca atcgggaaac tttcgtaatc
    14041 atatacggta gggtgccaaa ccatatcgta attatcgaag taataataat tatagtttaa
    14101 aaacggaaat gactttggtt ttagaattgc atacaccgta aaagctgaat aggaattttc
    14161 gatattttgc agccggttgc gatacgaagc ctgcactttt tcaggctcaa tcatatccat
    14221 aagtatcgcc gggtgtattg acgaaatata gtgatctgca gaatatgtct ttttatcggt
    14281 agtgtgtatg gcctcaatca gtttgttttc cacatcaatt ttgcaaacct cagcatttag
    14341 gattacatta ccgccactat tctgtataat ttcgaccata gcatcggcca gttgctgact
    14401 gccaccatta aagcgcgatg cgccctcaat acaaaattta gttaccagtg catgtatgta
    14461 gatgggtgta acatcgtatg caccagcata caaggtattg tcccatgcaa gtaccatttg
    14521 aagttttttg ttcgttgtaa atgatgaaat gtaatcgcca accgaaataa ggaatgaatc
    14581 atcgaaataa cccaggcttt cagttttaaa acgcaagttg aataagggta ttgcatcagc
    14641 aaggccgtat attccattca cataagcaat aatattttcg gtttcttcag ggaacgattc
    14701 cgacaacaat ctgatgtagt tttctttacc aacacccata tagtacttgc gatcatcgct
    14761 accgacatgc atcacatcga agccgtcttt attcaaaggt ttaatatcaa gtttatcggt
    14821 tatattcaaa tagttaaaaa gtttccgcag tactccacct tcttgaaaac cgctgatata
    14881 atgaataccc gtttcaaaaa gcacaccgtg ctttttaaag caatgcagtc cgccacccac
    14941 aatggaatgt ttttcgagaa tagtaacctt gtaaccctct ttcgaaagta agcatgcaca
    15001 taccaatcct cccagaccgc caccaataat tatgacattc tttcccatca gcagttttta
    15061 atatcgttaa taattgtgtt gatacccata aattcagagc aggttacaat agcaccaatt
    15121 gttactccta aaattccatg tgagttaata ttctgtccgg tcatatacag gttaggaatc
    15181 tttgtccttt gagaaaccag cgtttgggta gggaagtttt tatcccgcaa aacaccgtac
    15241 atagagcctt cttttgttgc agtataatcg cggtaggtta agggtgaaga agttgaataa
    15301 gactcaattt ctgacatgat tccaggaaag cttttttcaa gtgatgaaag cagtttttcg
    15361 gcttttcttt gcttaaattc caaatactcc acgcctctgc gatttacttt tgtgctctcc
    15421 cattgctcca cttcgtcgta acgcatatat ccaatcagca cagcactgct cgcatatccc
    15481 gaagcattat catcaaccgg tgcttggtgc atatacagat aattcagtgg ccaattctcg
    15541 cttgtataat tctcacattc ccaaacatca ttgcctgcat agtaatagaa gttggaattc
    15601 atatatttca ccctgttttt acgaaatgta atgtacacgg tgaagttcga tatcgagttg
    15661 gtgagattgg atatccgaac ccgcgttgca tttcttatta acgaagtgtc gattttatcg
    15721 attgtcgctt gagggtgaat gttcgaaata aatcttttgc cttcgataat ttcaccgttt
    15781 tttagcaaca cagaagtcat tttttcggag tcgcaattga attttaccac ttccgattgt
    15841 gtcattattt cgccacctga ttttctgatt gacgtagcca atgaagacgc aatacaatcg
    15901 ctaccaccaa caatccggta agcactctga atatagaaat tattaatcag ggtatgtata
    15961 tataatggtg ttatgtcctt tttgcctgca tataagggta aatttccaac aagaacattc
    16021 ctaagagttg tgtttttagt aatagaatcg atgaactcgt tgatgcttga ttttaccgac
    16081 tctattttca ggaaaacatt ttgctcaatt tccatcagat tgtatagtgg tgattcaagt
    16141 gcaatttcct gaattctgtc gacataggtt ttaagatgtt gtctttccgc tgggaatgac
    16201 tctgacaata catcaacaaa attaccgaat cccatggcat acttatatat cgaatccttg
    16261 tacgaaataa catcgtaacc gtcttcatcc agcttttgca atttgacatc ctcaagtagt
    16321 gagaaatatc tgaaaaacct gtgcattacc tgccccggat ccatactgcc gatataatgc
    16381 attccggtat cgaatttcac accaccgctg ttaaatgttt gcagacaacc gccaatctga
    16441 cgattttttt ccaggattag gactttaaaa ccgtttttaa caaggatata accgcaaagc
    16501 aatcctccca ggccacttcc aataattacc acatcgtatt tcgccattac ttcctgtttt
    16561 taactatcca aatacctttt ttattccctt cagaaacaac acaacccata tcaacaaata
    16621 atcttgcccc tgagttcatt tctgccacca aaaatataaa atcccgattt gccaacaagc
    16681 atttcctaat tgtttgttcc gaaggttcaa caccggctat caggtcaaat tcactgtaat
    16741 caatatcctc atctgcgggt tccgaatagt gtaaatttgg aggattcgaa acacagtttc
    16801 cggcaatatc taacttatct ctatcattgt cggtggcata cacctgcagg ttctttttta
    16861 ccagagatat catcaaagag actgttccaa acccacattc tgcaatacat actctgcctg
    16921 tatcaggcaa ggaagttatt aatgcttcaa aattactata gcttcgcatc aatctgcgtg
    16981 cactgcgctc aataaacgcg cccttgtaaa cataattatg tattacaaga ttgctgaaat
    17041 aagatggcgt ttcaattgca tcaaccatat tgccgtattc ttcaatgtac atctttctga
    17101 tatttcgtgc acgctccgaa taattatcac cgtacatttt atcatccggt tttatgcgct
    17161 gcaaaatttt tatcgttgct ttccccttcc ttaaaagcat ttcttttttt ggtaaggcat
    17221 gacccaagcc atgaacaata acaggaataa tatcgagatt tagtttttcg gccaaataaa
    17281 atgcccctcg gtggaaacgc agaatagaac agtcttcgga gcgcgtgcct tccggaaaca
    17341 ccataatgga ataccccttt tctaccattt gtcccagacg ggctatactg ttttcgatac
    17401 cggtggcaac aggataataa tcggcatagc gtatcattcg tccataaaaa ggggaattcc
    17461 atacccattc gttggtgagg ataacaattt taggagataa catcatgata tacatcaaat
    17521 cgatatgcgc ctgatggtta caaattatta ccccgggttg cgaaaagtct tcgttgtatt
    17581 cattgatgat ttcacgctta acaaacggaa tttttcttgt aaccaaatcg gttacatact
    17641 gcatagcctt gtggtatcgc agtttgttct tgtccgtttt tctgctgatc cccagtaaaa
    17701 agaacccaat gcaagttaat accagtgtgc ctaaaaggaa agcgcaaaaa ctataaaccg
    17761 tagcaaaaaa attcacaaat gttacaggta tcagtctttt tttaccgttt ttataggtaa
    17821 ttgcatggta aaatattggg ggtataataa acgatatcag caccacagaa atcatcccga
    17881 taattgtgac attacccagc gacttcatgg cagggtgttt tgcaaaaacc aaggtaccta
    17941 taccaacaaa catagttact gccgacaaaa caaccgccgt tttgtacgac gagagcagtt
    18001 tacgacgact ggcgtattca ctcatcatac cttccatcat aaatatggtg taatcatcgc
    18061 caagtccaaa tataaaagtc gcaagaatta tgttcacaat attgaatccc acaccaaaaa
    18121 ccgacataat acccaaaatc caaatccaac tgataagcat gggcaaaaac gcaatcaccg
    18181 acaattcgag acggccaaac gagattgtaa gaaaaagcag taccaggaaa ctgcatatat
    18241 aaagcactct gttaaaatcg tctgaaagca aatcaacgag cgattgcgcc acacttcctg
    18301 agtcgaatac aaaacaattg ctgcccgata atttccgtga tacttcagca gtgttatctt
    18361 ttccggtatt tataacactc acaaccatgg ctcgttcacc cgttacaatc agatagtctt
    18421 tcagaaaatt ttcggttatt atagaaaagt aagcggacgg ttgaacagta tatgtgctat
    18481 caagcgcgtt taggaaacca tcgaaagact cctttttgaa tccttgtttt acagcttctt
    18541 caacaaaaat atccttaaat gttacgctgc gactttccca gaattgattc catttctgaa
    18601 tctttgcttt ttgcatttgt gaagaaggca aaaactgtcc aatacccgaa atgctttgta
    18661 tcgtacctgc ttcccttaat gaatcaatca cgtctttttt gctctcatat tgctgtaatg
    18721 ccacatcgag agtagcagca tccgacacaa aataaaccga cttttctccc aacgtcgtat
    18781 aagcactcat ctcctcaaac gattcctttt gttcaggagt catgtagttt attttgttca
    18841 tctccgtttc gaagttcaca tcgaacgaaa aatagaaaaa cacaaaagtc agcagcacaa
    18901 cgactgtaat tatccatcgg ttgcggtcga tgcggtaacc tgaaatacga tcgatcacac
    18961 cagttttttc gacattgagg ttgggttgat ttttaaggaa atggggtaaa aatatcagga
    19021 caaacagtag ggtgccggcg agagaaaaag ccgcaaacaa tccgaaatcg cgcattgaat
    19081 cggcactgat gaacagtagg ctgagaaatg ctcccacagt tgtaatgctg cctgtaatca
    19141 ttggcgaaga aatatctctg agcacttcac cctgattgtg tgcatatttg gcgtggatca
    19201 aataatgcag tgaatagtta atagcaatac caaaaattac agaacccgcc ccaatagcaa
    19261 tggcagagat tgtaccttta agaaaatata gagctgcgag agccattaat gcaccaaaaa
    19321 gcaccgggac agcaatgaga acgatagatt ttacactgcg aaaaaaccat agtaataatc
    19381 ccaatatcaa cactactgat atcagtatgg caagataact gtcgttttta atctgtttcg
    19441 cattgctcac ccctaccgga gctgcaccaa aataccgtac gtcagcattg aaatgttttc
    19501 ggacggaatc aacactttca tcgaggtaat caaccagcac cgcattttcg gccgttttac
    19561 ttacggggtt gctcgctgta acaaaaagta aaatattggt actgtcgttc gaaaaaatat
    19621 aattattata aataatataa tcgctgctga tctgaaattc gttaagcctc gacaacaccg
    19681 gtgttgccag atgaagcggg tcggaaatga tttgttgctt tagcaccatc cctgcaggtg
    19741 aaataagcag ttttttattg tttgcaagaa ttttactgat gctgtcgcga ttaatcaacc
    19801 gatcgatgcg ctcataatcg gactcactca aataatatgg aagattctgg gtaataaaac
    19861 cggcaacatc gaatataagt tgctgatcga tcttgtaaaa aacatcatgt accagatttt
    19921 ttccatcagt aacatacttc aggtgattgg cgaagtagtc tgcagcttct aacagtgagt
    19981 cgcattgatt ttttttccct tgtgaagaaa tttttacaat aattcgttcc gatacaccaa
    20041 tattcttgta aagaaaattt attttctcat tgtttttttc tccgggcata aatccggaaa
    20101 tatcctcttc gagagtcaac cgcgaagcaa aataaccaaa cagtagcaac aatagcgctg
    20161 agatagtaaa aaccaaccat ttccgtcttg aaaaaaaatg atatatggaa agtactattc
    20221 ggctcatttt gtaattttac gcaccatttc tattttcaga tggtaaaaat aataaatata
    20281 gggaaacaga cagggtacat acgttaaaac ctgaaaccca tcctgaaact aaataccaaa
    20341 aattgcttac gtatattgtt ctgaccaaaa acatgattat ttacatcgag acttaccatg
    20401 gcacctgtat aaaaattttt tgtattcaca ctcaaagagc ttttaaagta cagatcgaaa
    20461 ggaagcttga ccttaattcc atgattaaaa cttaactgaa ggtttggtcc agctgcaatg
    20521 gcgttggaga agtttaccgg gcctaatacc agtgaatatc cataaccaag gcaattcgaa
    20581 aaagagagga aagtggcttt cctgaaatcg ttcaatccgg aataataagg tgtatatatt
    20641 ggaggtatga taggcaggct atccgattta atattactta tttcagggtt caatgtatag
    20701 aaaaacgaac cggcactttt ctcctgaatc tcggagtaat cgaatgcagc ttccattgaa
    20761 aaatcgtgat caaaaataag tttggctcgt aaacccactt caaaactcga caatccgttg
    20821 ttctgagggt aaattgaatc ggtagagaat ttggggaata aaatgctttg atcggaataa
    20881 taaaagccct tgcatcttga gaaataggct tcgagccata aaaaccttgt ttttataccc
    20941 atttcgaaac tattaaagcg cgtagggcca taacacagat tataatcctg atcggttgta
    21001 atgttgaaag aatacgatat tcggaatatc ttatatctgg cgcccagtgt aattgcaccc
    21061 t
//
LOCUS       DAOOQP010000046        21048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3079_length_21048_cov_6.686705, whole genome shotgun sequence.
ACCESSION   DAOOQP010000046 DAOOQP010000000
VERSION     DAOOQP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_21048_cov_6.686705"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLK75_06525"
     CDS             <1..919
                     /locus_tag="PLK75_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOY38938.1"
                     /translation="IKVDEPTMSMLFTINNSPFFGKEGIYVTSRHLRERLYRETEKNL
                     ALRIEETDSPDRLTVYGRGILHLSILVETMRREGYEFQLGQPQVIIKDIQGKKHEPIE
                     LLTVVVPEEFSGKVIEIASKRKGELLNIVPKNDRISIEFKIPVRGMIGLTNPVLTATE
                     GEAVIAHRFLGFEPWKGELLNKRNGVLVAIETGAVIPYSLNKLQDRGKFFVDPGVDIY
                     AGQIVGENTRFDDMGVNLTHTKKLTNMRASGSDAKMMIAPSVKFSLEECMEYILSDEY
                     IEVTPKSVRLRKILLNENDRKRNKLAQES"
     gene            complement(916..1992)
                     /locus_tag="PLK75_06530"
     CDS             complement(916..1992)
                     /locus_tag="PLK75_06530"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOY38939.1"
                     /translation="MRGILLITYICLAVCNLAIAQHYNSGNIEMEIRMISDSADHNAF
                     PDLVRYKKYFYCAFREADSHIPGENNLNGKIRVIRSKKGDKWETVTSFEIPKYDMRDG
                     KFVILPDGRLMMYFGCSEYKNGRLISRLTHVSYSSDGVHFTPPRPVEIDPAIKANFDW
                     VWSITWYDTVGYANMYQIKGKGGKWNVYLMKTTDGLHYSPVTSWDLGPRPNEARIRFA
                     PDGRMIVIVRRESGGPGILGMSYPPYTEWEWYDMSIKLGGPNFLVLSNNKILIGSRLF
                     KSKEVGEQMGKVNEDYRTGIFIASDLGEIRKVVELPSDGDTSYPGMVIYKDYLYVAYY
                     SSHEGKAKIYFAKILFSDIESLFD"
     gene            2196..4247
                     /locus_tag="PLK75_06535"
     CDS             2196..4247
                     /locus_tag="PLK75_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOY38940.1"
                     /translation="MDNQDNLNHDLLPEGSELQGNQENSSQESIAAADVESEQSNEET
                     NETENNTMAENENQPQPEKSEIIDSANETQFEPLPEFDADLSENQLDENDSELLKTEP
                     EEIDYSAYSKQELLQLLKQLIYEKPVNAIKLEIDSIKKAYYRSRQNEIVLLKKQFIDK
                     GGIAETFEIPRDTDEDYFKELLNDYKARKSVYNEQIEKEKQENLVLKKQIIEKIGLLA
                     NRDESLARTYDEFRDLQKQWKEIGLVPQADMQNILNSYQMQIEKFYDFIKIDKELRDL
                     DLKKNLDLKIELCNKAESLLIDPDVVEAYKKLQEYHQQWKELGPVPADKREEVWERFQ
                     VATRQINKNHQDYFLKIKEELENNLKQKEIVCEQAEALLLEPCDTIAQWEDISKKFTE
                     LQSIWKSIGMVPRKNNAAVYERFRAACNTFFENKKQFFSQIRDEQNNNLQRKIELCIQ
                     AESIKESTDWKKTTNDFLLLQKQWKEIGPVPRKQSQEIWLRFRKACDSFFNTKQNYFA
                     NIDNEFATNLNLKQTLINEVTSFSDTGSNSGNLEKLREFQNRWIEIGQVPHKEKERLQ
                     KDFRNAINELYTRLNIQKQSLDVEGFKQRIQILVDTGNIDGLRRERANIIQKQKTLDI
                     EITQLENNMSFFSSKAESLLIDVKRKIEKAKAEMEQLAEQKKTVDLAERELRSKIEKK
                     A"
     gene            4253..6553
                     /locus_tag="PLK75_06540"
     CDS             4253..6553
                     /locus_tag="PLK75_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY38941.1"
                     /translation="MKLRKVLLSVGMVFVFFAAMSQNGTMKGFVYDKATGEACIFANV
                     SIQGTNLGAATDVNGYFSINQIPAGKHTVIFTYVGYDSVAIDIDMVAGKLISEKVYMT
                     KSAVLLQETVISAEREERQTQVRTSIIKVTPAQIKRLPTVGSDPDLAQYLQVLPGVIF
                     TGDQGGQLYIRGGAPIHNLVLLDGMTVYNPFHSIGLFSVFDSDIIQSTDVYTGGFPAD
                     YGGRVSSVMDIRMREGNKKRFSGVFSASTFGTKILLEGPLKKFKENEGSASFIISAKT
                     SFLEQSSKLLYTYINEQGLPFNYTDLYGKLAINSRDGSKFNLFGFRFNDNVSYQEISD
                     LHWNSYGLGGNLVLVPTGSSVMIKANLSYSDYRIEMSDMNSAPRYSEMGGFNIGFDFL
                     YFLGKNEFVWGLQTLGFKTDFRYYNAVNRMLVENENSTEFAVYARYKHNFGNLIIEPS
                     MRLHYYATMSEVSPEPRLGVKYNVSETFRLKFAGGLYSQNLIAANSDQDVVNLFYGFL
                     TGSNNIPDTFNGEEVTSSLQKSQHVIAGFEYDITNRLTANIEGYLKNFSQITNINRNK
                     IYDDNAANYDKPDNLKKDLIIESGRAYGVDFLLKYDSKQVYLWLVYSLGWIERFDGII
                     TYSPHFDRRHNVNFVGTFKFGNNLLWNISARWNYGSGFPFTPTAGYYEQMPFYTNLNL
                     PYWETNGNLGIIYGDINSSRLPDYHRFDVTVQRTFHFTERVKLEVVLSCTNVYNRQNI
                     FYFDRVDHERVDQLPIMPSLGLSLTF"
     gene            6565..8166
                     /locus_tag="PLK75_06545"
     CDS             6565..8166
                     /locus_tag="PLK75_06545"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOY38942.1"
                     /translation="MGSTKYIFVTGGVTSSLGKGIISASLAKLLQSRGYKVTIQKLDP
                     YINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNVFTSQENNVTTGRIYQDVIN
                     KERRGDYLGKTVQIIPHITDEIKRRIQLLGTKYKLDFVITEIGGTVGDIESLPYIESV
                     RQLKWELNNDCIVVHLTLVPYLAATGELKTKPTQHSVKTLLEAGVQPDIIVLRTEHPL
                     NDDIKRKVALFCNVTKESVIESRNVKTIYEVPIRMKKEKLDITILKKLNMPAGHEPEL
                     KPWNEFLERLHHPKSEVNIGIIGKYVELKDAYKSINESLIHAGAANEVQVNLYHIHSE
                     MITPENVHEKLCNLNGILVAPGFGHRGVEGKITTVQYARTNNIAFLGICLGMQCAVVE
                     FARNVLGWKEAHTSEINSDTKYPVIDLMENQKKVTEKGGTMRLGSYTCRLVKESLAQK
                     AYQKQKISERHRHRYEFNNQYMKDFEAAGMKATGINPENSLVEIVEIPLHKWFIGVQF
                     HPEYKSTVLAPHPLFVEFVKAANTK"
     gene            8181..10058
                     /gene="yidC"
                     /locus_tag="PLK75_06550"
     CDS             8181..10058
                     /gene="yidC"
                     /locus_tag="PLK75_06550"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOY38943.1"
                     /translation="MDRNTILGLLVIGLIIVGYYFISKPSKAELEKQLQYRDSIQQAL
                     RADSLEKAKTLELKTDTVETDTVVVVADTIISDSAKAAVISQKYGVFAPAAQGENKMF
                     TVENQKMIISFSPKGGRVYSVQLKDFLTHDSLPVVLFSGDTTLFGLELMAGGTAVFTN
                     DLYFTPVNAADHIDAKSKDQKIAMRLYADTDKYVEYNYIVHPDSYMIDFNIVFHNMQN
                     VLSENVSYSKLQWSLMAPAQEKGRKWELQNTAVYVKMDDNEIEHLSLTKETDEFSSGG
                     KVHWIAFKQQFFSSVLIAKELADDPIVKQKTFTGANSKYLKQFAASFTFPVKRDSVQI
                     VPMQFYFGPNKFSILKKFDIKLEKLVPLGWGIFGWFNRFLIIPIFTWLGSFIGSYGLI
                     IFLLTIIIKLLLFPLTYKSYMSTAKMKVLKPQIDELTKKFPKGKEMERQQATMSLYKR
                     AGVSPMGGCIPLLLQFPILIALFNFFPASIELRQQSFLWAHDLSTYDSIVTFPFNIPW
                     YGDHVSLFTLLMAISLLVSTMMTNATQSGTQQMPGMKIMLYLMPVMMLFWFNDYAAGL
                     SYYYLLANLITILQTWVIRRFFVDEEKVLAKLEENKKKPVKKSKWQERLEMAAKQQGR
                     R"
     gene            10086..10787
                     /locus_tag="PLK75_06555"
     CDS             10086..10787
                     /locus_tag="PLK75_06555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOY38944.1"
                     /translation="MHAHEDLPLMFGEISPTYRLLNSLLSFGIDRIWRKKLVSAMPRN
                     LQSIVIDVSTGTADIPVILHKMQYSRITGVDPSEKMLTIAKKRIEKLNSPNNIELICA
                     GAENIPIAGNSADVVTVAFGVRNFDNLQQGFNEVFRILKNNSHFLILEFSLPQNTVLR
                     ILYKAYLYVVLQFFGGIISGNFAAYRYLRKSIIRFTENCDLVKELEGTGFTNIAKQNL
                     TGGIVSIYTAEKQSD"
     gene            complement(10961..11695)
                     /locus_tag="PLK75_06560"
     CDS             complement(10961..11695)
                     /locus_tag="PLK75_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOY38945.1"
                     /translation="MMKIIELLKLQNRAHRYKTKYDRGGIGFLLQSVGTGDTVLDIGA
                     HKGGYLYFFRKQIGESGKIYAFEPQENLFQYLKYIVTLLGWSNITVENIALSDNQGET
                     TLYIPNNKIKEGSSPGATIVEHPERLDFVKTQSISTDTLDSYCEKNSIKPVFLKIDVE
                     GNELQIFKGGLKILQKCKPKILVEIEKRHVGKQKAKETLDFLCSLGYNGYFIHGAKNK
                     PLEEFDFEKYQNPEDAENYCNNFIFL"
     gene            complement(11827..13521)
                     /locus_tag="PLK75_06565"
     CDS             complement(11827..13521)
                     /locus_tag="PLK75_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOY38946.1"
                     /translation="MKKIHLLLLAAIVLIAACNTKNQKKENEMEKQFVSFAEKHQKQI
                     EPLYKQLTEAYFKASITGNSEDFQKAAELELEFVKIYTNKDEFEQLKKYKESGEIKDS
                     LMLQELNTLYNSYLANQADPKMLQEMINLATGIEEKYNKFRAVVNGKQKSDNEIEEIL
                     STSTNSKEVEIAWTEVKKLGPVVSEDIIALVKMRNQLAQSLGFKNYHEMSLKLSDQDP
                     EEISKIFNELDELTRDAFTELKSEIDIYLAKKFNIKPEDLKPWHYQNRFFQEAPKMYE
                     VDLDKYYTDQNIEKLTIDYYSSIGLDITDMIAKSDLYEKPGKNQHAYCTSIDKNGDVR
                     VLCNIKPNYNWMNTMLHEFGHAVYDKYIDRELPFVLRDAAHTFTTEAIAMLFGRMASN
                     ATWMKDMNIIDEAEKNAISETGFRSLRLEQLVFSRWAQVMYNFEKSMYENPDQDLNKL
                     WWDMVEKYQMLNRPEGRDMPDWATKTHIASSPCYYHNYLLGELLASQLNYYICTQILK
                     TDDFNHQSFAGNEEVGKFLTEKVFMPGARYHWNEMIEKATGEKLTAKYYAMQFIDK"
     gene            13629..14885
                     /locus_tag="PLK75_06570"
     CDS             13629..14885
                     /locus_tag="PLK75_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY38947.1"
                     /translation="MKNKFYQLQLFLLCLTAFVFPLYIKATSITLLVLFILPFAFRSY
                     RGAFVQHLKSPGTYLFLMPMLVAFAGLINADQPASAFSNLEVYSSLFFFPFIFRTLNE
                     NPLKNKQQYIHSFFIFGILAAFFLCLGMAFINYFESFRKDYFFYSRLSSSIMSPNHLS
                     NYVLWAIMLILTDLAGLSNNLVLYSAKVYKAVVLLLLMCFLFLLASKAAFIIFCLMML
                     SFMIFLIVKKIIPLWQSVILCIVLLMLGLYLYQTTNIKGRVMFAYKVIENPSPKQSSS
                     QESTALRVSAFNASKDIIRDHFWFGVGTGDVQKTIVAYYQSHSKSGAYIHQTNPHNQY
                     LFSWIMNGVAGIIAILVMFGYLLVISARRKNIEPLFWVIYMILLFATDDILTIQIGVV
                     FFSFYTSLLLWSKPEINIHENNPNTA"
     gene            14885..16108
                     /locus_tag="PLK75_06575"
     CDS             14885..16108
                     /locus_tag="PLK75_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY38948.1"
                     /translation="MKDLWNKFRDITPIVFIISVAFLLPMHKHIIQVFLIIWGVFSLF
                     TYRWGKYIRHINRSFFPVVALILMYFLSITALLLFTENTDAGLFKLEVKLSFVIFPLL
                     LLPLVLFTSRQVRYLLLAFVAGCFLSDLICLGNAIYNYAEIGASAFYYTSLSLFHHTS
                     YFAMYHAFAIVILFFDYFYYSGRKLKLVYSGLILFLSVFVFLLSSKAGLIVLMIEFLA
                     LGFIILTDKFSFSKLLGVVAIVTLCGALLAGNYRVVSTTHQLVTKAPPSAAPKDSAES
                     TQARILVWNASVKVISENWLYGTSPGDASDKLVQKYHQLGFSQAELKKLNCHNQYLEV
                     LISLGVIGFIIFISIFIIPVSMYGFTRNLPLIFLLGITGINFLFESMLETQSGIIFFS
                     FFYTTFIYTFKNKQK"
     gene            16105..17328
                     /locus_tag="PLK75_06580"
     CDS             16105..17328
                     /locus_tag="PLK75_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOY38949.1"
                     /translation="MIPFSPPHIDEDIIQEVVAALRSGWITTGPRTKQFEKMLTGFNR
                     NKSTLCVNSATAGLELVLRWFGVGVGDEVIIPAYTYCATANVVLHCGAVPVMVDIVPE
                     TFNIDTKQIVAKITERTKVIIPVDIGGYPADYDEINAIVSSAEVMKLFKPQNSVQEKL
                     QRILVMADAAHSLGALYKSRPTGTMCDITVFSFHAVKNLTTAEGGAIAFNLPEPFDCE
                     EVYRYFNILSLHGQSKDALAKMQGKASWRYDVLMPGYKCNMTDIQAAIGIVELSRYRD
                     KILERRKAIFEFYFNAFKEFTWAMLPVFDDGSSKSSYHVFLLRIAGFEETQRDRMIEE
                     IFNRDVAVNVHFQPLPLLSVYKKLGYAMSNFPNAYSNYKCEISLPVYYDLTHEMCQTV
                     VTAVIEAYMSVSCNK"
     gene            17356..17943
                     /locus_tag="PLK75_06585"
     CDS             17356..17943
                     /locus_tag="PLK75_06585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOY38950.1"
                     /translation="MKRVFDIIISFLLLIFLLPLFIVISIVIIAGSKGGVFYCQQRVG
                     KNNTDFRIFKFRTMFINSDTKGLLTVGMKDSRITSAGQWLRKYKIDELPQLFNVLFGS
                     MSLVGPRPEVRKYVDLYSADQLKVLSVKPGITDYASLKYHNENDVLSKAQNPEEMYIG
                     KIMPEKIELNLKYIAESSFLTDLKIIIKTIAVIFT"
     gene            complement(17948..18751)
                     /locus_tag="PLK75_06590"
     CDS             complement(17948..18751)
                     /locus_tag="PLK75_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HOY38951.1"
                     /translation="MIFSKNIKLLRKRRKRTQDDVAFTLEMKRSTYSGYENEVAQPGI
                     DALIAISNYFNISIDTLLKVDLSVLSESQLRQIELGYDTFFKGSEIRVLATTVNSENE
                     ENIEMVNETAKAGYTLGYADPEFIKRLPVFTLPFLSHNKKYRSFQISGDSMHPIPDGA
                     FVIGEFVQNWYSLKDGDACIVITREDGIVFKCVENKIKNLGKLGLYSLNPLYEPFHIE
                     ASEVTEIWKFVNYFSNEMPDPIPAEKEVINTIASIKQDLCEIKQKLNID"
     gene            18833..19474
                     /gene="plsY"
                     /locus_tag="PLK75_06595"
     CDS             18833..19474
                     /gene="plsY"
                     /locus_tag="PLK75_06595"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOY38952.1"
                     /translation="MQLTLFIVLPVIAYLIGSFSSAVWYGKWFRGIDLRNEGSKNAGA
                     TNAFRVLGASVAIPVFITDAAKGFASVYLVYFLNFDALNEWFWVWGTILGICSVLGHI
                     FPLYTGFRGGKGVACMLGVLLALQPFIALCCFGVFALVFLLFKIVSVGSLVAAISYPV
                     FNFFIFETDSLTLRVFSLLTATLVIIMHHKNIRRLVSGTEKKLSFRPHPGKKA"
     gene            complement(19476..>21048)
                     /locus_tag="PLK75_06600"
     CDS             complement(19476..>21048)
                     /locus_tag="PLK75_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY38953.1"
                     /translation="NGSLPAEFYITTIVMLAFVAIAIYFIIRRNKINNLIFFGFFFFI
                     ITISLYLHIIPIEGRIIAADRYSYLPHLGLFILLALVFEKLIFRHSRFNIFSTVFLAI
                     LSMSMSIAVYSRTKVWTNTQTLFQDVVTKDPTAAFAYLNLAASHLQANQTDSAINCFN
                     KSIQLDSLNINAYYNRGLAYSDKKNYKMAMSDFNSFVRLAKSNKTKAIGFAQIGELYR
                     ASGKDSIALSYFNQALHYDSTLSIAYNKRGIHYLNIQDYGNALQDFEKAIKFNHFNSE
                     AINNKGMVLLLQGHIDESILYFSEALRLSPEYVLAYDNRAYAYSLAGDNDNALKDYNK
                     EIDLNPNNVEVYIKRGRVFASMKQYQDAIRDFDRVLQRKPTDFIALTNRAYAYYYSDQ
                     FEKARTGFSYIAGLYPENAASWQNMGWYFMQVKEYANALSAFEKSIRIDTALINSYIN
                     MGYVCIELKDYVSADKYLNQSLALNPKHPEALYFTGELKRLSGKAGIACEYYSKAVTA
                     GSKQAVEAQALYCPN"
BASE COUNT         6181 a         4083 c         4334 g         6450 t
ORIGIN      
        1 aattaaagtt gatgaaccta ctatgagtat gttgtttaca atcaacaatt ctccgttttt
       61 tggaaaagaa ggtatttatg ttacatctcg gcatctgaga gaacgcctgt atcgtgaaac
      121 cgagaaaaat ctggcactca ggattgaaga aaccgactca ccagaccggc ttaccgttta
      181 cggtaggggt atattgcatt tatcaatatt ggtggaaacc atgcggcgcg aagggtatga
      241 atttcaactg ggtcaaccgc aggtaataat aaaggatatt cagggtaaaa aacacgaacc
      301 tatagaactg cttaccgtgg ttgttcccga agaattttca ggcaaggtta tcgagattgc
      361 aagtaaacga aaaggagaac tgctgaatat agtccccaaa aacgatcgta tttctattga
      421 atttaaaatt ccggtgcgtg gtatgatagg tcttacaaac cctgtactga ctgccactga
      481 aggagaagca gtaattgctc accgtttttt gggtttcgaa ccctggaaag gtgaactgct
      541 caacaaacgc aacggagttc tcgttgccat cgaaacagga gcggttatac cgtattctct
      601 taataaactt caggaccggg gcaagttttt tgtggatcct ggtgtggata tctatgcagg
      661 acaaatagtg ggtgaaaaca cccgtttcga tgatatggga gttaacctga cgcatacgaa
      721 aaaacttacc aatatgaggg cctcaggttc cgatgctaaa atgatgattg ctccttcggt
      781 gaaattctct ctggaagagt gcatggaata tattctgtcg gatgagtata tcgaagttac
      841 accgaaatcg gtcaggctca ggaaaattct cctgaatgaa aacgaccgca aaaggaataa
      901 attggctcag gaatcctagt cgaaaaggct ctcaatatcg gagaatagta tttttgcaaa
      961 gtatattttt gccttccctt catgactgga ataataggca acatacaggt agtctttata
     1021 aatgaccatg cccggatagc tggtgtcgcc atcgcttggc agttctacta cttttcttat
     1081 ttctcccaga tcgcttgcta tgaagatacc tgttctgtaa tcttcgttaa ccttgcccat
     1141 ttgttccccg acttctttgc ttttaaataa ccggcttccg atgagtattt tattgttcga
     1201 caaaaccaaa aagttcggac cacctaattt tatactcata tcataccatt cccattcggt
     1261 atagggtggg taactcattc cgagtattcc cgggccccct gattcccggc gcacgattac
     1321 tatcattcta ccatcggggg cgaaacggat acgtgcctcg ttgggtctcg gacctaaatc
     1381 ccaactggtt acgggtgaat agtgcaggcc atcggtggtt ttcatcaaat atacattcca
     1441 tttacctcct ttccccttta tctggtacat gtttgcatat ccaacagtgt cgtaccaggt
     1501 aatgctccac acccaatcga aattggcctt aatggcaggg tcaatctcca cggggcgcgg
     1561 tggggtgaaa tgcaccccgt cgctggaata ggaaacgtgg gttaaacggc tgatcagcct
     1621 cccgtttttg tattcggagc aaccaaaata catcatcagc cttccgtcgg gaagaatgac
     1681 gaatttgcca tcgcgcatat cgtattttgg aatttcgaaa cttgtaaccg tttcccattt
     1741 gtctcctttt ttcgaacgga tgactctgat tttaccattt aaattatttt ctcctggaat
     1801 gtgggaatca gcttcccgaa atgcacaata aaagtacttc ttatatcgga caaggtcagg
     1861 gaatgcatta tgatcggcgc tatcactaat catgcgtatt tccatttcaa tgttaccaga
     1921 attgtaatgt tgagcaattg ccaaattaca aacagcaaga catatatatg taattaacaa
     1981 tattcctctc ataggaattc attataaggc attctaaaca aagctaataa aatattccga
     2041 aaacaaatcc aaaccttatt atcgtagtaa aaccgatata aaactgtatt ttgaagaatt
     2101 tattgaaaaa tctttgtcta ttcgttctgt tatttctatt ttcgtaacct tattataatt
     2161 tgtactaata gctaaacaaa agtaatttta ccgacatgga caaccaagac aatctgaacc
     2221 acgatttgct gccggaagga agtgaattgc agggaaatca ggagaactct tcacaggaaa
     2281 gcattgctgc cgcagatgtt gaatcggaac aaagcaatga agaaactaat gaaaccgaga
     2341 acaacacaat ggcagagaac gaaaatcagc ctcaaccgga gaaatctgaa attattgatt
     2401 ctgcaaatga gactcaattt gaacccctgc ctgaatttga tgccgactta tcggaaaatc
     2461 aacttgatga gaatgactct gaattattga aaacagagcc tgaagagatt gattattcgg
     2521 catattcgaa gcaggaatta ttgcagttgt taaagcagct tatctacgaa aagcctgtga
     2581 acgcaataaa attggaaatc gactccatta aaaaggcata ctacagaagt cgtcagaacg
     2641 aaattgtgtt gctgaaaaaa cagtttatcg acaaaggtgg tattgccgaa acattcgaaa
     2701 tcccgagaga taccgatgaa gattacttca aggagttact caacgattat aaagcacgta
     2761 agtctgttta taacgagcag attgaaaaag aaaaacagga aaatctggtt ttgaaaaaac
     2821 agattatcga aaaaatcggc ctgcttgcca accgcgatga gtcgttggct cggacctatg
     2881 atgagttcag agacctgcag aagcagtgga aagaaatcgg actggtacca caggctgata
     2941 tgcagaacat actgaattcg taccagatgc agattgaaaa gttctacgat tttattaaaa
     3001 tcgacaaaga gcttcgtgat cttgatttaa aaaagaatct cgacctcaag atagagcttt
     3061 gcaacaaggc tgagagcttg ctgattgatc cggatgtggt ggaagcttat aaaaagcttc
     3121 aggaatatca ccaacaatgg aaggaactgg ggcctgttcc ggccgataaa agagaagagg
     3181 tttgggaaag atttcaggtt gcaacccggc aaatcaataa aaatcatcag gattactttc
     3241 tgaaaataaa agaagaactc gaaaataacc tcaaacaaaa ggaaattgtt tgcgaacaag
     3301 ccgaagcatt attgttggaa ccttgcgata ccattgctca atgggaagac atctcgaaaa
     3361 agtttaccga actgcagtcg atatggaaaa gcatcggaat ggtacctcga aaaaacaatg
     3421 ctgctgtata tgagcggttt cgtgctgcct gcaacacatt tttcgaaaat aaaaaacagt
     3481 ttttctcaca aattcgcgat gagcagaaca acaacctcca gcgcaaaatt gagctgtgca
     3541 ttcaggctga atcgattaaa gaaagtactg actggaaaaa gacaaccaac gattttctgt
     3601 tattgcagaa acaatggaaa gaaatcggac ctgttccgag aaagcaatct caggaaatct
     3661 ggctgcgatt tagaaaagca tgtgatagtt ttttcaatac taagcaaaat tattttgcaa
     3721 acatcgataa cgaatttgca acgaacctga atctgaagca aaccctcata aatgaagtga
     3781 cttcttttag cgacacgggc agtaacagtg gcaatcttga aaaactcagg gaatttcaga
     3841 accgctggat tgaaattggg caggttcctc ataaagaaaa agaacggttg caaaaagatt
     3901 ttcgcaatgc aataaatgag ttatatacgc ggttaaatat tcagaaacaa tctcttgacg
     3961 ttgaggggtt caaacagcgg attcagatac ttgttgatac tggaaacatc gatggcttgc
     4021 gtagagaaag ggctaatatc attcagaagc aaaaaaccct tgatattgaa ataacccagc
     4081 tcgagaacaa catgagtttt ttcagcagta aggctgaaag tttgctaatt gatgtgaagc
     4141 gcaagattga aaaagcgaaa gctgagatgg agcaactggc agagcaaaag aaaactgttg
     4201 accttgctga aagagagctt cgttcaaaaa tcgaaaagaa agcatgatga atatgaaatt
     4261 gaggaaagta ttgttaagcg tgggaatggt gtttgttttt tttgctgcga tgtcgcaaaa
     4321 cggaacaatg aaaggttttg tatacgataa agctaccgga gaggcgtgca tctttgccaa
     4381 tgtttcgata cagggcacaa acctaggtgc cgccaccgac gtaaatggat atttctcaat
     4441 aaatcagata cctgcaggaa agcatacggt gatttttact tacgtgggtt acgattctgt
     4501 ggctattgat attgatatgg ttgctggaaa actgatatcc gaaaaagtgt atatgacaaa
     4561 atctgccgtt ttacttcagg aaactgttat ttcagcagaa agagaagaaa gacagacgca
     4621 ggtacgcacc tctattataa aagttacccc ggcgcaaata aaaaggcttc caacggtggg
     4681 aagcgatccc gaccttgcgc agtatcttca ggtgttgccg ggtgttatat ttaccggcga
     4741 tcagggtggt caactctata ttcggggggg agccccaata cataatcttg tactcctcga
     4801 cgggatgacg gtatataacc cttttcactc tatcggcttg ttttcggtgt tcgattccga
     4861 tattattcaa agtaccgatg tgtatacagg aggatttccg gccgattatg gcggcagggt
     4921 gtcatcggtt atggatattc gaatgcgtga aggaaacaaa aaacgctttt caggtgtatt
     4981 ttctgccagc acatttggta caaaaatttt acttgaaggc ccattgaaaa aatttaaaga
     5041 aaatgaaggg agtgcatcct ttataatttc ggcgaaaacc tcttttcttg agcagagcag
     5101 taaactgctg tacacctaca tcaacgaaca aggcttaccg ttcaattaca ccgatttata
     5161 cggaaagctt gcaataaatt caagggatgg cagcaaattc aacctgtttg gttttagatt
     5221 caacgacaat gtcagttatc aggagataag cgatttgcac tggaactcct acgggttggg
     5281 aggaaacctt gttctcgtgc ctaccggaag ttcggtaatg attaaagcca atctctcgta
     5341 ttcggattac cgtatcgaaa tgagtgatat gaacagtgct ccccgctaca gcgaaatggg
     5401 aggttttaat atcggtttcg attttctgta ttttcttggt aaaaatgaat ttgtttgggg
     5461 cttgcaaacc ttgggattta aaaccgactt caggtattac aacgcagtaa accgcatgtt
     5521 ggttgaaaac gaaaattcca cagaatttgc tgtgtatgca cgatataaac ataattttgg
     5581 gaacctgatt attgaaccta gcatgcgctt gcattactat gcaactatgt cggaagtttc
     5641 acctgagccc aggctggggg taaaatataa tgtttccgaa actttccgac ttaaatttgc
     5701 gggtgggcta tattctcaaa acctgattgc tgcgaactcc gatcaagatg tggttaacct
     5761 gttttacggt ttcttaacgg gaagcaataa cattcctgat acttttaacg gggaagaggt
     5821 aacaagcagc cttcaaaagt cgcagcatgt tatcgccggc tttgaatacg atataacaaa
     5881 taggcttact gcgaacatcg aaggatatct gaagaacttc agccagatta ccaatatcaa
     5941 ccggaataaa atatatgatg ataatgcagc aaattacgac aaacccgata atctgaagaa
     6001 agaccttatc attgaaagcg gcagggcgta tggtgttgat tttctattga aatacgactc
     6061 aaaacaagtg tatctctggt tggtgtattc attgggctgg attgaacgtt tcgatggaat
     6121 tattacctat tcaccccatt tcgacagacg gcacaatgtt aattttgttg gaacctttaa
     6181 gttcggcaac aatttattgt ggaacatcag cgcccgatgg aattacggat caggctttcc
     6241 gtttacacct accgcaggtt attatgaaca aatgccattt tatacaaacc tgaatctgcc
     6301 ttattgggaa accaacggta atctgggaat tatttacggt gatattaaca gctcacgcct
     6361 tcccgattac caccgttttg atgttactgt gcagcgaaca tttcatttta ccgaaagggt
     6421 taaattggaa gttgttttaa gttgtactaa tgtatataac cggcagaata tattttattt
     6481 cgaccgtgtc gaccatgaaa gggtcgatca gttgcctatt atgccaagtc tgggcttaag
     6541 tctaacattt taataccatt tgccgtggga tcaacaaaat acatatttgt aacaggaggg
     6601 gttacctcct cactaggtaa aggaattatc tcggcatctt tggcaaagtt gctgcaatcg
     6661 cggggctata aagtaaccat ccagaaactt gacccgtata tcaatgttga ccctggtact
     6721 ctgaaccctt atgaacacgg cgaatgctac gttaccgaag acggcgctga aaccgatctt
     6781 gaccttgggc attacgaacg ctttctgaat gtttttacca gtcaggaaaa caatgtaacc
     6841 acaggtcgaa tctatcagga tgttataaac aaggagcgcc gaggtgatta ccttggcaaa
     6901 acagtgcaga ttattcccca tatcaccgat gagataaagc gcagaattca acttttggga
     6961 accaaataca agttagattt tgtaataacc gaaattggag gaaccgtagg tgatatagaa
     7021 tcgttgccct acatagaatc ggtacgtcaa ttgaaatggg aattgaacaa cgattgcatc
     7081 gtggttcact taactctggt tccctatctg gcagccaccg gcgaactgaa aacgaaaccg
     7141 acacagcact cggtgaaaac acttctcgag gcaggagtgc aacccgacat cattgtcctt
     7201 cgcactgagc atcctctgaa tgatgatatt aaaaggaaag ttgccctttt ttgcaatgta
     7261 accaaagaat cggtaatcga atcgagaaat gtgaaaacca tttatgaggt tcccatcaga
     7321 atgaaaaagg aaaagctgga cattaccatt ctgaaaaagc tcaatatgcc tgccgggcat
     7381 gaacccgagc taaaaccctg gaacgaattt cttgaaaggc ttcatcatcc aaaatccgaa
     7441 gtaaatattg gtattatcgg taaatacgtc gaactgaaag acgcgtataa atccattaat
     7501 gaatcgttga ttcacgcagg ggcagcaaac gaagtgcagg tgaatctgta tcatattcac
     7561 agcgaaatga ttacaccgga aaatgttcat gagaaattgt gcaacctcaa tggaatactg
     7621 gttgcaccgg gtttcggaca caggggagtt gagggaaaaa tcactacagt gcaatatgcc
     7681 cgtaccaaca acatcgcttt tcttggtatt tgcctgggaa tgcaatgtgc ggttgtggaa
     7741 tttgcgcgca atgtacttgg atggaaagaa gcgcatactt ccgaaatcaa ttccgatacc
     7801 aaatatcctg taattgacct gatggaaaac cagaagaaag ttaccgaaaa aggcggtacc
     7861 atgcgattgg gctcctatac gtgcagactt gtgaaagagt ctcttgcgca aaaagcctat
     7921 caaaaacaaa aaatatcgga acggcatcgt cacaggtatg agtttaacaa tcaatacatg
     7981 aaagactttg aagcagcagg aatgaaagct accggtatca accccgaaaa ttcacttgtc
     8041 gaaatagttg aaataccttt gcacaagtgg tttattggtg ttcagtttca tccggaatat
     8101 aagagcaccg tgttagctcc tcatccatta ttcgttgaat tcgttaaagc agccaatact
     8161 aaataagata aaattaacac atggatagaa atacaatttt aggtctgttg gtgatagggt
     8221 taattattgt tggatattat ttcatcagca aacccagtaa agcagagctt gaaaagcagc
     8281 ttcaataccg cgacagcatt caacaggcat taagagccga ttccctggaa aaagcgaaaa
     8341 cccttgaact gaaaacggac actgttgaaa ccgatacggt ggttgttgtt gcagacacaa
     8401 ttatttccga ttcagccaaa gctgccgtta tatcgcagaa atatggcgtg tttgctcctg
     8461 cagcgcaggg cgaaaacaaa atgtttacgg ttgaaaatca aaaaatgatt attagttttt
     8521 cacccaaggg aggcagagtg tattctgtgc aactgaaaga ttttcttaca cacgattcgc
     8581 ttccggtggt gttgttttcg ggtgacacaa ccttatttgg ccttgagctg atggctggtg
     8641 gaaccgctgt ttttaccaac gacctgtatt tcacccctgt taatgccgct gatcatatcg
     8701 atgccaaatc gaaggaccag aaaattgcga tgaggttata tgctgatacc gacaaatatg
     8761 ttgagtacaa ttacattgtg catcccgaca gttacatgat tgatttcaat atcgtgtttc
     8821 acaatatgca gaatgtactt tccgaaaatg tttcttactc taagttgcag tggtcgctga
     8881 tggcaccggc tcaggagaaa ggcagaaaat gggaactcca gaacactgca gtgtatgtga
     8941 aaatggacga taacgaaatt gaacacctga gcctgaccaa agaaaccgat gagttctcga
     9001 gcggcggtaa ggttcactgg attgcattca aacaacagtt cttttcatcg gttcttatag
     9061 caaaggagct ggctgatgat cccatcgtaa aacaaaaaac atttaccggg gcaaattcaa
     9121 aatacctgaa acaattcgca gcatcgttta catttccggt aaaaagagat tctgttcaga
     9181 tagttccaat gcaattctat tttggcccca ataagttctc gatactgaaa aaatttgata
     9241 ttaaacttga aaaactggtt ccgctgggat ggggtatttt cggttggttt aacaggtttc
     9301 ttattattcc gatttttacc tggctcggca gtttcatagg cagttatggc ctaattattt
     9361 tcctgcttac aattattatt aaactgctgc tattccctct tacttacaag tcgtatatgt
     9421 cgactgcaaa aatgaaggtc ttaaagcctc agattgatga attaacgaag aaattcccta
     9481 aaggcaaaga gatggagcgt cagcaggcaa ccatgagttt gtacaagcgg gctggtgtta
     9541 gtccaatggg aggctgcata cccttattgc ttcagttccc gattttaata gcattattca
     9601 atttttttcc tgcttctatt gaattgagac aacaaagctt tctttgggca cacgatcttt
     9661 ctacatacga ttctatagtt acttttccgt ttaatatccc atggtacggc gatcatgtaa
     9721 gtctgtttac tctgctaatg gctatttctc ttctggtttc cactatgatg accaatgcta
     9781 ctcagtcggg aacacaacag atgcccggaa tgaaaatcat gctttatctg atgcctgtaa
     9841 tgatgttgtt ctggttcaac gattacgctg caggactcag ttattattac ctgcttgcaa
     9901 accttattac catacttcag acttgggtta tcagaaggtt ttttgtagat gaagaaaaag
     9961 tacttgccaa acttgaggaa aacaagaaga agcccgttaa aaaatcgaaa tggcaggaac
    10021 gtctcgaaat ggcagcaaaa caacagggaa gaagataaca gttacgcgga ttaaatccga
    10081 ttatcatgca cgcacatgaa gatctgccgt tgatgtttgg tgaaatctct ccgacatatc
    10141 gcctgttaaa ttccttatta tcgtttggaa tcgacaggat atggagaaag aagctggtgt
    10201 cggccatgcc gcgaaatctg caaagcattg taattgatgt atcgacaggt acggcagata
    10261 ttcctgtaat cctgcacaaa atgcaatatt caagaattac aggagttgac ccttctgaaa
    10321 aaatgctcac aatagctaaa aaacgtattg aaaaactgaa ttcgccaaac aatattgaac
    10381 ttatttgtgc aggtgccgaa aatattccca ttgccggcaa tagtgcagat gttgtaacgg
    10441 ttgcttttgg agttcgaaat ttcgataatt tgcagcaagg gtttaacgag gtattccgga
    10501 tactgaagaa caattcacac ttcctgatac ttgaattctc cctgccgcag aatactgtcc
    10561 tccggatttt atacaaggcg tatttgtacg ttgtgcttca gtttttcggg ggcattattt
    10621 ccgggaattt tgcagcatac cgctaccttc ggaaatcaat aatccggttt acagagaatt
    10681 gcgacctggt gaaagaattg gagggtaccg gttttacaaa tattgcaaag caaaacctga
    10741 ccggtggcat tgtaagtatc tacacagccg aaaagcagtc agattgaaaa ttgccatcac
    10801 aagataactt cataaatttt cagaatgcaa ctgcgcaata agtgtatctg ctatttaata
    10861 cattacccaa cattcttatg tcatcagtgg cttgactata ttaaagccca aacctaaacc
    10921 cgttattccc atttcagagg aatataccag tgccggtgtt ttataaaaat ataaagttat
    10981 tacagtagtt ctctgcgtct tcggggttct gatatttctc aaaatcaaat tcctcaagcg
    11041 gtttgttttt cgctccgtga ataaaatatc cgttgtagcc gagcgaacac aagaagtcaa
    11101 gagtttcttt tgccttctgt ttgcctacgt gtcgcttttc aatttctaca agaattttag
    11161 gcttgcattt ttgtaatatt ttcaatccgc ctttaaaaat ttgtagttca ttcccttcaa
    11221 catcaatttt aagaaataca ggttttattg agtttttttc gcagtatgaa tcaagcgtat
    11281 cagttgaaat ggattgggtt tttacaaagt ctaacctttc ggggtgctcc acaatggtgg
    11341 ctcctggcga agatccttcc tttattttgt tattcggtat atacagggtt gtttctccct
    11401 gattgtcgga aagcgcaata ttctctacag taatattact ccaacccaat agggttacaa
    11461 tatatttcag atattggaat agattttcct ggggttcaaa agcataaatc tttccgcttt
    11521 caccgatttg cttacgaaaa aaataaagat acccgccttt gtgcgcccct atatcaagca
    11581 ctgtgtcgcc ggttcccacc gactgaagta aaaacccgat tcctccgcga tcgtactttg
    11641 ttttgtagcg gtgcgccctg ttttgcagtt ttaacaactc aataattttc atcattctta
    11701 tgctaaggtt tatgaatatt tagggcaaac atacatattt ttccaactgc aacactataa
    11761 aaaactactg ccgatgctca tcgtgatagg ttgcatcggc aggtgagtca tcaggcaaat
    11821 gtctgattat ttatcgataa actgcatggc gtagtacttg gcggtaagtt tttcaccggt
    11881 ggctttttca atcatttcgt tccagtgata tctcgcaccc ggcatgaaca ctttttcggt
    11941 aaggaatttg cccacttctt cgttgcctgc aaagctttga tgattgaagt cgtctgtttt
    12001 caatatctgc gtgcagatat aatagttcag ttgagaggca agcaattctc cgagcaaata
    12061 gttgtggtaa tagcatggcg atgaggcgat gtgtgttttg gtagcccaat ccggcatatc
    12121 gcgaccttcg gggcgattta gcatttgata tttttcaacc atatcccacc ataatttatt
    12181 caaatcctgg tcggggtttt cgtacatgct tttttcgaaa ttatacatta cctgtgccca
    12241 gcggctgaac acaagctgct caagtcgtaa cgacctgaaa ccggtttccg aaatagcatt
    12301 tttctccgct tcgtcaatta tattcatatc tttcatccat gtagcattcg aggccatacg
    12361 tccaaaaagc atggcaattg cctcggttgt aaatgtatga gccgcatcac gaagtacaaa
    12421 gggaagttct cggtcgatgt atttatcgta aacagcatgg ccaaactcat gtagcatagt
    12481 attcatccag ttgtaattgg gttttatatt gcacagaacc cgaacatcac cgtttttatc
    12541 gatgctggtg caatatgcat gctggttttt acccggtttt tcgtataaat cgctttttgc
    12601 aatcatgtcg gtaatatcaa ggcctatcga tgaataataa tcgatagtca gcttctcgat
    12661 attctggtcg gtatagtatt tgtcgagatc aacttcatac atcttaggag cctcttggaa
    12721 aaaacggttc tggtaatgcc agggcttcaa atcttcgggt tttatattga atttttttgc
    12781 aagataaata tcaatctcag actttagctc tgtaaatgca tcgcgggtaa gctcatccaa
    12841 ttcgttaaat attttgctta tctcttcagg gtcctgatcg ctgagtttca gactcatttc
    12901 gtggtaattc ttaaaaccta gggattgtgc caattgattg cgcattttaa cgagagcaat
    12961 gatatcctcc gaaacgaccg gacctaattt tttaacttct gtccaggcta tttcaacttc
    13021 cttgctattg gtgcttgttg aaagtatttc ctctatttcg ttatctgact tttgcttgcc
    13081 attgacaact gcccggaatt tgttgtattt ttcttcaatg cctgtagcca ggtttatcat
    13141 ttcctgaagc atttttgggt ctgcctgatt tgccagatat gagttgtaca gcgtgtttag
    13201 ttcctgaagc ataaggctgt cttttatttc gcctgattct ttatacttct taagttgttc
    13261 aaactcatct ttgtttgtgt aaatcttaac aaattcaagc tccagctctg cagctttctg
    13321 aaaatcttcg gaattacctg tgattgatgc tttgaaataa gcttcggtga gttgcttgta
    13381 aagcggctca atttgctttt gatgtttctc tgcaaagctt acgaattgtt tttccatttc
    13441 gttctccttt ttttggtttt tagtattgca tgctgcaatt aatacaattg cagcgagtaa
    13501 taatagatgt atttttttca ttattgtatg atttaaaatt cccataaaat tatgaaatta
    13561 cccaacactt tggaattaat gatgcccaat taacagtatt ttaaccattt cttttatttt
    13621 tgtgttgtat gaaaaacaaa ttctatcagt tgcaactttt tttactttgc cttactgcat
    13681 ttgtttttcc actgtacata aaagcaactt caattacgct acttgtactt tttattttgc
    13741 catttgcgtt ccgaagctat cgcggtgcat ttgttcaaca tttgaagagc ccgggcactt
    13801 atcttttcct gatgcctatg ctggtagcct ttgcaggact tattaatgcc gaccagcctg
    13861 catcagcctt cagtaacctt gaagtgtatt cctcattgtt tttcttccca tttattttca
    13921 gaacccttaa cgaaaaccct ctgaagaaca agcagcagta tattcattct tttttcattt
    13981 ttggcattct cgcggctttt ttcctttgtt tggggatggc ttttatcaat tatttcgaga
    14041 gctttcgcaa ggattacttt ttttactcca gactttcgag ctcaattatg tcgccaaacc
    14101 atttgtcgaa ttacgtcctt tgggctatta tgcttattct caccgatttg gcaggcttgt
    14161 cgaacaacct tgtgctatat agtgcaaaag tatataaggc tgttgttctt ttgttgctaa
    14221 tgtgtttttt atttcttctt gcttcaaaag cggcattcat catattttgc ctgatgatgt
    14281 tatcgttcat gatttttctt attgttaaga aaattattcc actctggcaa tcggtaatcc
    14341 tatgcattgt tttattgatg ctgggattgt atctatacca aactaccaac attaaaggca
    14401 gggttatgtt tgcctacaag gtcatcgaaa atccttcacc caagcagtct tcaagtcagg
    14461 agtccacagc attgagggtt tcggctttta atgcgtcgaa agatattatc cgcgatcatt
    14521 tttggtttgg ggttggcacc ggcgatgtac agaaaactat cgtagcgtat tatcagagtc
    14581 attcaaaaag tggtgcttat attcaccaga cgaatccaca taatcaatac cttttttcgt
    14641 ggattatgaa tggtgttgca ggcattatcg caatattggt gatgttcggt tacttgttgg
    14701 taatatcggc tcgtcgcaaa aacattgaac ctttgttttg ggtaatttat atgatattgc
    14761 tgtttgcaac cgatgatatt ctcacaatcc agattggcgt cgtattcttt tcgttttata
    14821 cgtcgttgct gttgtggagc aaacctgaaa ttaatatcca cgaaaataac cccaatactg
    14881 cctgatgaaa gatctctgga ataagtttcg tgatattact ccgattgtat ttattatttc
    14941 ggttgcattt ttgttgccaa tgcacaaaca tattatacag gtattcctta ttatttgggg
    15001 cgtattttcg ttatttacct acaggtgggg caaatatatc cgacacatca accgatcttt
    15061 tttcccggta gttgcattga tactgatgta ttttctttca ataacggcac tattgctctt
    15121 taccgaaaat actgatgcag gtttgtttaa actggaagtg aaactgagct ttgttatatt
    15181 ccctttactg ctacttccat tggttctgtt tacttcacgt caggtaagat accttcttct
    15241 ggcatttgtg gcaggttgtt tcctttctga cctgatctgt cttggcaacg ctatatataa
    15301 ttatgcagaa atcggggcaa gtgcatttta ctataccagc ctttcgttgt ttcatcatac
    15361 aagctatttt gctatgtatc atgcttttgc cattgtaata ctattcttcg attatttcta
    15421 ctattcgggg agaaagctta agctggtata ttccggattg attctctttt tatcggtttt
    15481 tgtttttctt ctttcatcaa aggctggttt gatagtgtta atgattgagt ttttggctct
    15541 cggattcatt atactaaccg ataagtttag ctttagtaaa ttactgggag ttgttgcgat
    15601 tgtgacgtta tgcggagccc tgctggccgg taattacaga gtagtatcaa caacgcatca
    15661 attggttacc aaggcacctc catcggcagc accgaaagat tcagcagaaa gtacgcaggc
    15721 aagaatactt gtatggaatg catcggtaaa agttatttcc gaaaactggc tatatggcac
    15781 ttctcccggt gatgcatccg ataaacttgt acagaaatac caccagctag gtttttcgca
    15841 ggcggagtta aaaaaactga attgccataa tcaatacctt gaggttttga tatcgcttgg
    15901 cgtaataggc tttataatat ttatttcgat ttttattatt cctgtatcaa tgtatgggtt
    15961 tacacgtaat ttacccctta tttttttgtt ggggattacc gggatcaact ttttgtttga
    16021 atcgatgctc gaaacacagt ccggtatcat atttttttct ttcttttaca ctacttttat
    16081 ttacactttt aaaaacaaac agaaatgatt cctttttcac cgcctcatat cgatgaagac
    16141 ataattcagg aggtagtggc tgcacttcgg tcgggatgga ttaccaccgg accacggaca
    16201 aagcaattcg agaaaatgct taccgggttc aacagaaaca agtcgacctt atgcgttaat
    16261 tctgcaactg ccggacttga actggtttta cgctggtttg gggttggtgt tggcgatgaa
    16321 gttattatac cggcttacac gtattgtgct acggcaaatg tagttttaca ttgcggagct
    16381 gtaccagtta tggttgatat tgttccagaa acattcaata tcgatacgaa gcagattgtt
    16441 gcaaagatta cagagcgtac aaaagttatt attccggtcg acattggcgg atatcctgcc
    16501 gattacgacg aaatcaacgc tattgtctct tctgcggagg tgatgaaatt attcaagccg
    16561 caaaacagcg tacaggaaaa attgcaacgg atacttgtaa tggcggatgc tgcacactca
    16621 cttggagcct tgtataagag cagacctacc ggaacgatgt gcgatatcac agtattttcg
    16681 tttcatgccg ttaaaaatct tactacagcc gaaggtggtg ctattgcttt taatttgcct
    16741 gaaccattcg attgcgaaga agtttatcgg tattttaata tccttagcct gcacgggcaa
    16801 agcaaagatg ctctggcaaa aatgcaggga aaagcctcat ggcggtacga tgttttaatg
    16861 ccaggatata aatgcaatat gacggacatt caggctgcaa ttggcatagt ggagctgagt
    16921 agatatcggg ataaaattct tgaaagacga aaagcaattt ttgagtttta ttttaatgca
    16981 tttaaagaat tcacgtgggc tatgctcccg gtttttgatg atggtagctc aaaaagctct
    17041 taccatgttt ttcttttgcg tattgcaggt tttgaagaaa ctcaacgcga taggatgatt
    17101 gaggaaatat ttaaccgcga tgttgctgta aatgtccact ttcaacccct tccattatta
    17161 tccgtgtaca aaaaactcgg gtatgccatg agtaattttc ccaatgcata ttcaaattac
    17221 aaatgcgaga taagtttgcc agtgtattat gatcttaccc acgaaatgtg ccaaacggtg
    17281 gtaacggcgg taattgaggc atatatgtca gtatcctgca ataagtagct gattacaaag
    17341 tttaaagtca gacaaatgaa gcgcgtattc gatataataa tttcttttct tttgttaata
    17401 ttcttattgc ctttattcat tgtgatatca atcgtaatta tcgcaggaag caaaggtgga
    17461 gtgttttatt gccagcagag ggttgggaaa aacaatacag acttcagaat cttcaaattc
    17521 agaacgatgt ttatcaattc cgatacgaaa ggactcctga cggttggcat gaaagatagc
    17581 aggattacct cggcaggaca atggctgaga aaatataaaa tcgatgaact tccgcaatta
    17641 ttcaatgtgc tttttggtag catgagttta gtaggtccac gtcctgaagt cagaaagtat
    17701 gtcgatttat attctgccga ccagttgaaa gtgcttagtg ttaagcccgg aataaccgac
    17761 tacgcttcgc tgaaatatca caatgaaaac gatgtgctat caaaagctca aaaccctgaa
    17821 gagatgtaca ttggtaaaat tatgccggag aaaatagaat taaacctgaa atacattgca
    17881 gaatcctcgt ttctaaccga tttgaaaata attataaaga caattgcagt aatttttact
    17941 tagctgacta atcaatattc agtttttgtt ttatttcgca gaggtcttgc ttaatactgg
    18001 caatagtatt gataacttct ttttcagccg gtatggggtc gggcatctca tttgaaaaat
    18061 aatttacaaa tttccaaatt tcggttactt ccgatgcctc gatatgaaac ggttcgtaca
    18121 gtggatttaa agagtatagc ccaagttttc cgaggttttt tattttgttt tcaacgcatt
    18181 tgaatacaat cccgtcttcg cgtgttatta caatgcaggc atctccgtct ttaagcgaat
    18241 accagttctg cacaaattca cctattacaa aggctccatc aggaataggg tgcatgctgt
    18301 cgcccgaaat ctgaaagctt ctgtattttt tattgtgcga taaaaaaggc agtgtgaaaa
    18361 cgggaagcct ttttataaac tcagggtcag cataacccag cgtgtacccg gcttttgcgg
    18421 tttcgttcac catctcgatg ttttcttcat tttcggagtt taccgtagta gccaacaccc
    18481 tgatttcaga tcctttgaaa aaagtatcat accccagctc aatttgccgt agctgacttt
    18541 cgctgagcac tgataagtcc actttgagca aagtgtcgat gctgatattg aaataattgc
    18601 taatagcaat aagtgcatcg atgccaggtt gtgcaacttc gttttcgtaa ccactatatg
    18661 tgctgcgctt catttctaaa gtaaaagcaa catcatcctg agtccgtttt cgtcttttcc
    18721 gtaataattt aatatttttg ctaaaaatca ttgcagttaa aatttaatca aaaatacgat
    18781 tataatttaa acaatgcaat gataaaaata taattttgaa ataaaataca gaatgcagtt
    18841 aaccttgttt atagtattac ctgtaattgc ctatctgatt ggctctttct cctccgctgt
    18901 ttggtacggt aagtggtttc ggggaattga tctgcgcaat gagggcagca aaaatgctgg
    18961 tgcaaccaat gcctttcgcg ttctcggagc ttctgtggca attcccgttt ttataactga
    19021 tgcggcaaag ggatttgcaa gtgtgtacct tgtatatttt ttgaatttcg atgccttaaa
    19081 cgaatggttt tgggtttggg gtacaatatt gggtatatgt tcagtgttag gccatatttt
    19141 tccattatat acagggttcc gagggggaaa aggtgtggct tgtatgcttg gtgtgctgct
    19201 tgcacttcag ccttttatag ccctttgctg tttcggagtt tttgctttag ttttcctgtt
    19261 atttaaaatt gtttcagtag gttctcttgt ggctgcaatc agctatcccg tttttaattt
    19321 ttttattttt gaaactgatt cacttacctt acgtgttttt tcgcttctta cggctactct
    19381 ggtgataata atgcatcaca agaatatcag gcgattagtt tccgggaccg agaaaaaatt
    19441 gagcttccgt cctcatccgg ggaaaaaagc atgactcaat tggggcaata caatgcttgt
    19501 gcttcaactg cctgtttgct tccggcagta accgcttttg aataatattc gcaggctata
    19561 ccggctttcc ctgatagacg ttttaattca ccggtaaagt acaatgcttc agggtgcttc
    19621 ggattcagtg caagcgattg gtttaagtat ttatctgcag atacataatc tttaagctca
    19681 atgcagacat atcccatatt tatgtaagaa ttaattaagg ctgtgtcaat acgtatcgat
    19741 ttttcgaatg ccgacagagc attggcatac tccttcactt gcataaaata ccaccccatg
    19801 ttctgccatg aagcagcatt ttcaggatac aaacctgcga tatagctaaa accggttctg
    19861 gctttctcga actgatcgct gtaataataa gcataggcgc ggtttgtaag ggctataaaa
    19921 tcggtgggtt tacgctgaag caccctatcg aaatcgcgaa tagcatcctg atattgcttc
    19981 attgatgcaa aaacacggcc tctcttaata tagacttcaa cattgtttgg atttaggtca
    20041 atttctttat tgtaatcttt taaagcattg tcattgtctc ctgctaacga atatgcataa
    20101 gctcggttat cgtaagcaag aacgtattca gggctcaggc ggagggcttc cgaaaaatat
    20161 aaaatcgatt catcaatatg gccttggagt aacaacacca ttcctttgtt gttgatagct
    20221 tcagagttga aatggttgaa ttttatggct ttttcaaagt cctgcaacgc attcccgtaa
    20281 tcctgaatgt tcaggtaatg aatgcctctt ttattgtaag cgatcgacaa ggtactgtcg
    20341 taatgcaggg cctgattaaa atacgacaaa gcaatactat ctttccccga agccctgtac
    20401 aattctccaa tctgtgcgaa ccctatggct ttggttttat tgctttttgc caatcgcaca
    20461 aaagagttga aatcgctcat ggccatttta taattctttt tatcagaata ggccagtccc
    20521 cgattataat atgcattgat attcagtgag tcaagttgaa tcgatttatt aaaacagtta
    20581 attgcgctgt cggtctgatt tgcttgcaaa tgcgaagcag caagattcag gtatgcgaaa
    20641 gcagcagtcg ggtccttggt tacaacatct tggaaaaggg tctgtgtgtt ggtccaaacc
    20701 tttgtccttg aatagactgc aattgacatc gacatggaca gtatagctaa gaaaactgta
    20761 ctgaagatgt tgaatctgct atgcctgaaa atcaactttt cgaaaacaag tgcaagtaaa
    20821 ataaacaggc ccagatgcgg taagtatgag tatctgtcgg ccgcaataat acgcccttca
    20881 atgggaatga tgtgcaggta aagtgagatg gttataataa aaaagaagaa cccgaaaaaa
    20941 atcaaattat ttattttatt cctccttatt ataaagtata tggcaatggc cacgaatgcc
    21001 agcattacta tagttgtaat gtaaaactct gcaggcagac ttccgttt
//
LOCUS       DAOOQP010000047        19617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3376_length_19617_cov_5.301912, whole genome shotgun sequence.
ACCESSION   DAOOQP010000047 DAOOQP010000000
VERSION     DAOOQP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3376_length_19617_cov_5.301912"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLK75_06605"
     CDS             <1..794
                     /locus_tag="PLK75_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOY38954.1"
                     /translation="PNPVMLRDPEFGPVRIRAFGVYEFRVKDAATFIKDIVGTDGEFT
                     TEKVTNQLRNIIVTRFTDAVAESKIPVLDMASNYNEFSQFVGKAIIEEYAEYGLEMTK
                     FLVSNISLPPNVEEALDKRTSMGVIGDLGKYTQFQAANAMEAAATNPGGGAASEGMGM
                     GMGFAMAGQMMNAMNQGNQQQQQQQQQAAVPPPIPGQVKYFIATNGQQSGPFDIATLS
                     QYAKTGQLTKDTLVWKEGMASWGKAGEQTDLSAVFGSVPPPLPPA"
     gene            823..3099
                     /locus_tag="PLK75_06610"
     CDS             823..3099
                     /locus_tag="PLK75_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38955.1"
                     /translation="MKTTFFLIGLSLLIQTGVYAQWTNIDEPLHRRGSRVQYYYTGDD
                     VIANTGLSIFRFNQSGNDWEYAGDNLFNQYPYRYFDNHFVWENQLFCSVDMHLMKYDA
                     DNNTWIEQEYTLPELMPEAYLIQTFSSTDEFVYIVLQYQESGINYFTIYYTTDFVTWN
                     EGLTQEDVGLAYFRFRMNNHYIAWANENNDVLYSDDGETLDTLHFGSVPKRPAPDQWM
                     EYLQGESIGDYIFYKGTDDRMYRYSISGDVWDTIQNNFLQPTFVIDFTVAQGLLICST
                     INGPNLIALGSNDGGQSFFPISGNMGPLPILSDLCYVGNNQYWLTSMLSDVLYSGNSG
                     VDINIRNEGFTAGMNYIVPFDNKLYTFITGYGVSKADPENLEFEYDNEGTEPFIGILA
                     SDGLYSTGPRLYYSLIEDISNGAMGIYYKDDEASEWTRIDAISDLVGVRFLGSDNAGN
                     LYLFLRISQSKDVITRYYRLSPELVLTELLNIPGAATGTGTPYNIIAGGANDLYYFYT
                     NDEMTDKLYRSLDSGETWIELNMGYPNYTFVFDFRKIDGSFANVASLVNDAGELVFIL
                     RTKFGVPVTYENILLKYNLSDDMFTQLDASGFPQDMLYACSLGKSDGFIPYCVATNFG
                     FFYSDSDLDNWMPDSFEHGYRTGMVPNGIVDLGNRIIVSTLCHGIWYSDFYNSIVQEG
                     INNVGVFPNPAQKTISVMGLNAGDKVIVYNTQGVELMQFTAGTSSQSVNVESFEQGMY
                     FIKVNHNNVERIMRFIKE"
     gene            3215..4573
                     /locus_tag="PLK75_06615"
     CDS             3215..4573
                     /locus_tag="PLK75_06615"
                     /inference="COORDINATES: protein motif:HMM:NF003761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOY38956.1"
                     /translation="MKNHISVKQGLDIRLYGEANKIIQNETASSSYALVPDDFPGLTS
                     KPVAQPGDTLKIGSLVFVDKYKPEIRFVSPVSGILKDIVRGEKRKIMKYLIIPDDTND
                     TEVFEKPDVNTLTREQSVSFLCNAGLWPFVTQRPYGVIANPETTPKSIFISFYDTAPL
                     APDYEFFLKDYLQQMIHGLKIIRPLTTGKIHLGFAKNSGLIQSFLGLDFVETHTISGP
                     HPAGNVGTIINKIDPINKGEIVFTLMPQDIVSIGNTALSGKYSPERIIAVTGSELTHT
                     GYVKTRHGASIKSLLNGNVEHDPDAVRIVSGNVLTGTNVGYKGYLGYKHPQITAIPEG
                     KKYVMFGWMKPGFKKFSNSHTYFSWLFPKRRYRIDTNLNGGLRNLVVTGEYEKVCPFD
                     IYPQYLIKAIIAKDIDKMEQLGIYEVIEEDLALCEFVCTSKTQIQAILREGLDLIRSE
                     MN"
     gene            4595..5776
                     /locus_tag="PLK75_06620"
     CDS             4595..5776
                     /locus_tag="PLK75_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOY38957.1"
                     /translation="MKSVRKLLDKAKPNFESGGKLYFLHSFFDAMETFLFVPDKVTEK
                     GAHIRDAIDMKRTMILVVVALMPAFLFGVWNTGFQFYFSRGIDAGFWQIVGYGLKMVI
                     PLYLVSYGVGVGVEVIVAQIRKHEVNEGVFVTSFLIPLLLPADIPLWMLAVATAFAVL
                     IGKEIFGGTGMNIMNPALLARAFLFFAYPAWMSGDKVWVSGLTQGEGVVDGFTGATPL
                     AQAAAGSPHFTDGLGNTLSTMDLFIGAIPGSIGETSKLAILLGAALLLFAGIASWRVM
                     FSMLIGGMSVGILMNVLGNTPYMQIPFYDHLLIGSFLFGMVFMATDPVTAAQTNTGKF
                     IYGFLIGVMIMVIRVLNPAYPEGTMLAILLMNVFAPLIDHYVVQANISKRLKRFKQIK
                     A"
     gene            5784..6491
                     /gene="nqrC"
                     /locus_tag="PLK75_06625"
     CDS             5784..6491
                     /gene="nqrC"
                     /locus_tag="PLK75_06625"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOY38958.1"
                     /translation="MNTNSNKYIFIYSVIMVVVVAFVIAFIATQLKPRQQKNIEIEKK
                     RNILSTIHVESTTENAEQLFSKHVVDMFSVNVAGEIIKGTNPLYCDKKAEFTKPAPQR
                     ELPVFVAKSADGNTKYIIPLLGKGLWGPISGYVCLNEDMKTVYGAIFMHKSETPGLGA
                     EISTSWYQAQFDGKQIYDSTGNFTSVRVVKGGADKSDKHAVDAITGGTITSNGLNEML
                     FDGLKQYDSYFKKMANK"
     gene            6497..7123
                     /locus_tag="PLK75_06630"
     CDS             6497..7123
                     /locus_tag="PLK75_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOY38959.1"
                     /translation="MALFKNLRLITDPINKNNPITVQVLGICSALAVTVKMKPAFVMA
                     LSVIFVTAMSNVIISLLRNTIPNRIRIIVQLVIAAALVIIVDEVLKSFVFDISKQLSV
                     FVGLIITNCIIMGRLEAFALSNKPWQSFLDGVGNGAGYGLILLIVSFFRELLGSGTIW
                     DYKVIPDAWYTAGYMNNGLMILPPMALIVVGIIIWVQRLRNKNLIENK"
     gene            7135..7752
                     /gene="nqrE"
                     /locus_tag="PLK75_06635"
     CDS             7135..7752
                     /gene="nqrE"
                     /locus_tag="PLK75_06635"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOY38960.1"
                     /translation="MENIFNILVKSIFIDNMIFAYFLGMCSYLAVSKTVKTSVGLGVA
                     VIFVLLITVPVNYLLEMYVLREGAMAWLSPAMADVDLGFLRFILFIAVIASMVQLVEM
                     VVEKFAPALYSSLGIFLPLIAVNCAILGGALFMSERDYANIGEATVFGLGSGIGWFLA
                     IVGLAAIREKIKYSHIPQPLQGLGITFIITGLMGIAFMGFMGIKL"
     gene            7775..9025
                     /gene="nqrF"
                     /locus_tag="PLK75_06640"
     CDS             7775..9025
                     /gene="nqrF"
                     /locus_tag="PLK75_06640"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOY38961.1"
                     /translation="MSQNIMILAVSILVFLLIVLLLVSILLYAKAKLMPKGKVLIEIN
                     GKSTVETEPGSSILGTLGNNKILLPSACGGGGTCGMCKCRVIEGGGSILPTETGFFSR
                     KEQQNYWRLGCQVKVRQNMKIEIPEEVMGIKKWECEVVSNRNVATFIKEFVVKLPEGE
                     FLDFRSGGYIQIDVPKIDVDFAKDIDVEQEFRDEWDKFGMWSLKMKNSEETFRAYSMA
                     NHPAENNIIMLNIRIATPPWDRKKGTFMNVNPGVCSSFVFSRKTGDKVFISGPYGEFF
                     IKNTQREMMFIGGGAGMAPMRSHIFHMFNTVKTNRKTTFWYGARSKREIFYEDEFRKI
                     ENEFPNFAFNIALSEPKAEDNWTGYVGFIHQVIYDNYLNKHQEPEEIEYYLCGPPLMN
                     DAVQKMLDNLGVPKEMIAFDDFGG"
     gene            9037..10050
                     /locus_tag="PLK75_06645"
     CDS             9037..10050
                     /locus_tag="PLK75_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOY38962.1"
                     /translation="MKNRINNMNLLPILILSVWLGACTGSNKNYYNCEGRTQGTMYHV
                     IYEHNEDISDEINSFLVDFSKSLSNYDRNSVISKFNYNAKDFKPDSLLITMLTASQEV
                     WQKTEGAFDITVAPVANLWGFGWEKPGNIDTSRIVALMQYVGMNKISISGQQITKQYP
                     EVMIIGNGIAQGLSVDCLAYFLESKGISNYLIEIGGEVCAKGYKANSELWKIGIDKPI
                     NSSYSNRQNQTVLSLTDWSLATSGNYRKFVEMGQKTYGHSINPKTGYPAKTDVLSVTV
                     ATRQCMYADAYATAFMVIGFEKSFELAKKIPDLEAYFIYIAPDSTHQVAYTDGFDKFL
                     LDE"
     gene            10111..10575
                     /locus_tag="PLK75_06650"
     CDS             10111..10575
                     /locus_tag="PLK75_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38963.1"
                     /translation="MNNLLFEKNVFTGDLAEYDKVLEFISFFANINEEDYISYNEDKT
                     FEYTPRFYEFIESLFRAGLVEDYNKMREFLSNACFDFEESDEYVSWIHEMNKIICRPE
                     LLSKANLSFIRKAFLTLIRLEYRIPGSWGIDIEVCTWLKLLKRLNQIRPELA"
     gene            10726..12636
                     /gene="dnaK"
                     /locus_tag="PLK75_06655"
     CDS             10726..12636
                     /gene="dnaK"
                     /locus_tag="PLK75_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOY38964.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIANSEGKRTTPSVVAFVD
                     NGERKIGDPAKRQAITNSQKTIYSIKRFMGERFNNLSNEVSRVPYKVVAGDNNTPRIV
                     IDDRKFTPQEISAMVLQKMKKTAEDYLGQEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LRVARIINEPTAAALAYGLDKKNRDMKVAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDNMVIDWLAAEFKNDEGVDLRKDPMATQRLKEAAEKAKIELSSSVTTEIN
                     LPYIMPVDGIPKHLVKTLTRAKFEQLVDKLINATIEPCRKALKDSGYSASEIDEVILV
                     GGSTRIPAVQKKVEDFFGKTPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETLGGVMTKLIESNTTIPTKKTETFTTAADNQPSVEIHVLQGERSMASDNKTIGR
                     FHLDGLPPAMRGTPQIEVTFDIDANGILKVSAKDKGTGKEQSIRIEASSGLSDDEVKR
                     MKAEAEANAEMDRKAKERIDKINQADSMIFQTEKQLKEFGDKIPEAKRKPIDSALDQL
                     KEAHKNQDVQSIDKAIADLNAAFQAASEDMYKAQNQPQGDPTQQQSGSSQNTGNSKPE
                     QEVTDVDFEEVK"
     gene            complement(12686..13066)
                     /locus_tag="PLK75_06660"
     CDS             complement(12686..13066)
                     /locus_tag="PLK75_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOY38965.1"
                     /translation="MNVRVDKYLFSVRLYKTRNEATTACRNGSVIVNDNRVKPSYIIK
                     QGDKIEVRINPIFRTFTVIQINDKRCSNALVSDYISETTPTAELERLRIARSVRNSKP
                     QTRPNKKDRRILGKLFEQNEIDPS"
     gene            13159..15606
                     /locus_tag="PLK75_06665"
     CDS             13159..15606
                     /locus_tag="PLK75_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38966.1"
                     /translation="MKNIYIFIITLIMAMYGSIAFAQNQKMIGEIQDSDEKIIPGAFD
                     EELFSKVLIKRLNHLMEDSLFLEGYVLHEFFKAPAEEHAKIMAEDGSASIEGKGEYAT
                     IKSRLELAGGSGSGFELVAKSSIRNGDANFTYNQLADFCINRWLTSSKTREPLLRQQY
                     FFAGIGAKFDTDSTKIYISMYVGNWESFLQGSERYDELPFQVSKKKFGLKLPEPEMNR
                     KMLSKAKRRFPNLLELQSGLSVNENGEIYFKYSDLRGFKRIMRNSKDGLAVDIVQTCQ
                     FDNCGETNIVDFGRRSRGIMLKPVLSKKMFKNNIAPPEGTGKRKKVKKLEVYMGQMPE
                     GLNPDDAELNLVFIQNKYIVTNIHPTYIDKAIYNYAQKIDLLPDTLLPDGISEYFPSA
                     TSTKLSFRIPFEQGKFNYDSDDMIPVLTALNEPAFIINKIHIAAYSSLEGSEAENAAL
                     QKKRANSIVSALEENQNASIIDSITTAPNFEQLKTDVKGTPFEKVAEMNYKEAIQYVN
                     AHAGEMEYLLKNHRYADVTIWVTYDVEGDKEQAYVLDQFNKEVAAERYDKALAIQKYI
                     FKRVVEGRYNSKAVSGMVIPNIKPCVGLNMNKICLERIVANEPVDSTYLERIEDLQTM
                     DGENIYVRYNDIYCEVMLSNMDNTNVVEELQGRIDNLYSIFPNKMTVDLLNIELQYQI
                     MEVYKDSLGFDNPVVIAGLNKIKQIVGATAINWENALKMAGIFINHYDYEYAYQLLWP
                     WVDKDPVNTSLLYSYVSLASKVEYRCYSNRFLMAMEKLKQHDPKGFCKLFRGDKFSVQ
                     VYANEKLKELYCNSCKQ"
     gene            15660..17000
                     /locus_tag="PLK75_06670"
     CDS             15660..17000
                     /locus_tag="PLK75_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY38967.1"
                     /translation="MKSILLFLLLSILLGNCNAQDFFVKLSIPDYPERELSFCSQFGD
                     KSRIIETFRTDKNGYVSFNTTDLEIGVYRVYFDSEVYFDIIINKENISLSLLANAPLE
                     SMEVIESIENRVLYNFLKQNNAINQKLDVLEYFLAKYAKGKLYKRVTKEYQEVIKEQA
                     YLADNVTAGEKNTFAAGMIQYYKTPIIPKEVAQNGNENMFLREKFFTYFPFDDTLLVN
                     SSVYSSAVLNYLKLFAVSGDYGASVQGFKSASERILNYTRPREAIFSHVLKILVDGFE
                     SLQLFDIADYLLENYGSKCVDDDNLSLRYKNITELPLQSKAPVVEYIDKAGNITDSYE
                     NKNTIIVFWATWCDHCRQVIPELSEYFETNHSNSISVVLISLDTDKSTLDNFVRENSI
                     DLPVICDYRGWEGTYVELFAIYATPFFYVVGPDGAIVSKPHDYNELLNVLKSLK"
     gene            16997..17617
                     /gene="yihA"
                     /locus_tag="PLK75_06675"
     CDS             16997..17617
                     /gene="yihA"
                     /locus_tag="PLK75_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOY38968.1"
                     /translation="MTPLIKSISFVKSSPDYKSCPSPEKHEYAFAGRSNVGKSSLINM
                     LARHKGLAKTSGVPGKTQLINHYLIDDSWYLVDLPGYGYAKTSKKNRELFQKMLQGYL
                     LNRNNLVSVFALIDCRIPPQNSDISFMNFMVTNGVPFALVFTKTDKLGPLVLGRNIEH
                     YKHTLLEIWEDLPPIFITSSEKSTGGDELIDYINTLNQQIQLKKRP"
     gene            complement(17651..18568)
                     /locus_tag="PLK75_06680"
     CDS             complement(17651..18568)
                     /locus_tag="PLK75_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOY38969.1"
                     /translation="MKRFLLGVVFTVILLPIAGFAQQLPLYSQYFLNNFVINPAVAGS
                     EKYSPVRLSVHKQWVGINDSPSTQSISGHTLLQNKTVGIGGFIFNDSFGPVSHTGIQA
                     VYSYHFAFNRLNRVALGLAAIAFQYKMDQRFFQLTIYDDPSITYMIERTIVPDATFGV
                     YFYGKKYYAGITAAHLFQSKLKINRNLDDNTMVRHYYIMGGYKFDFQSAPSFKIEPAL
                     MMKMTEVTPIQLDFNVKLYYNDDYWFGMSIRPNDAFIAAIGLKINRYYLGYSYDFTFS
                     DLSNYTIGSQEIVFGVNVGEPIKRGRSFF"
     gene            complement(18796..>19617)
                     /locus_tag="PLK75_06685"
     CDS             complement(18796..>19617)
                     /locus_tag="PLK75_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOY38970.1"
                     /translation="VINPAAAGSNKDYSPLRLSIHRQWVGIDQTPSTQVISCHHLLSN
                     KEMGIGGMIFHDSFGPVRTIGSQFTYAYHLRATRGIKVAFGVSAILMQYIISLDQEDF
                     HSFEPVLLRNRMSILVPDGNLGAYAYSKKWWAGLSVAHIFQSNLKITSTYMDEKSNKM
                     VRHYFLNGGYRFSFPSAKSLQIEPSSLIKFTETTPIQIDLNIRMIYNEDYWAGMSIRP
                     GDSFIFMIGMNYKGYYFAFSHDFTFSELSNFTIGSEELTFGWNIGDKSYKSSRFY"
BASE COUNT         6126 a         3570 c         4163 g         5758 t
ORIGIN      
        1 cacccaaccc tgtaatgctc cgtgatcctg aattcgggcc agtaagaata cgggcatttg
       61 gagtttatga attcagggta aaagacgcag caacttttat taaggacatt gttggtaccg
      121 atggcgaatt taccacagaa aaagttacca atcaactcag aaatataatt gttacccgtt
      181 ttaccgacgc tgttgctgaa tcaaaaattc cggttctcga catggcttca aattataatg
      241 agttttcgca gttcgtcgga aaagccatta tcgaagagta tgccgaatat gggcttgaaa
      301 tgacaaaatt cctggtatcg aacataagcc ttccacccaa tgtggaagaa gccctcgata
      361 agcgtaccag catgggtgtt attggtgatc tgggaaaata tacccagttt caggcagcca
      421 atgcgatgga agcagccgca accaaccccg gtggaggtgc tgcatccgaa ggtatgggca
      481 tggggatggg ttttgctatg gccggtcaga tgatgaatgc aatgaatcag ggaaaccagc
      541 aacagcaaca acaacaacaa caagcagcag ttccgccacc aattccggga caggttaagt
      601 actttatagc cacaaatggt cagcaaagcg gtcctttcga catagctaca ttgtcgcaat
      661 atgcaaaaac aggccaattg actaaagaca ctctggtttg gaaagaaggc atggcatcct
      721 ggggaaaagc cggcgaacag actgacctca gcgctgtttt tggaagtgtt ccgccgccgc
      781 taccccccgc gtaaccctaa gttaaaccta aatactaacc ccatgaaaac aactttcttc
      841 cttatcggtt tgtccctttt gattcaaacc ggtgtttatg cgcaatggac aaatattgat
      901 gagccacttc ataggagagg ttcaagagtg cagtattact acaccggcga cgatgtgatt
      961 gctaatacag gcctttctat tttccggttc aatcaatctg gaaatgactg ggaatatgcc
     1021 ggtgataatt tattcaatca gtacccttac cgctatttcg ataatcattt tgtctgggaa
     1081 aatcaattgt tttgttcagt tgatatgcat ctgatgaaat acgatgccga caacaatacc
     1141 tggattgaac aggaatatac ccttccggaa ctaatgcctg aagcctatct catacaaact
     1201 ttttcttcta ccgatgaatt tgtgtatata gtgttacaat atcaggagtc gggaatcaat
     1261 tatttcacaa tttactacac aaccgatttt gtgacctgga atgagggatt aactcaggaa
     1321 gacgttggat tagcatattt caggttccgt atgaataatc attatattgc atgggcaaac
     1381 gaaaataatg atgttttgta ttctgatgat ggtgaaacac tcgacacact gcattttggc
     1441 agtgttccca aacggccggc acccgatcaa tggatggaat accttcaagg cgaaagcatt
     1501 ggcgattata ttttctataa agggaccgac gaccgaatgt atcgctatag cattagcggt
     1561 gatgtgtggg atactattca aaacaatttc cttcaaccaa cttttgttat tgatttcacg
     1621 gtagcccagg gattgctcat ttgcagcaca attaacggcc cgaaccttat tgctttgggt
     1681 agtaatgatg gtgggcagtc attcttcccg atttctggta atatggggcc gctgccaata
     1741 ttatcggatt tgtgctacgt aggaaacaat cagtactggc ttacaagtat gttgtcggat
     1801 gtgctctatt ccggtaatag cggagtggat attaatatcc gcaacgaggg tttcactgcc
     1861 ggaatgaatt acattgtacc tttcgacaac aaactctaca catttattac aggttatgga
     1921 gtgtcgaaag cggaccctga aaaccttgaa tttgagtacg ataacgaagg aactgaacct
     1981 ttcatcggga ttcttgcatc agatggcctt tactctacag gaccccgctt gtattattcc
     2041 ctgattgaag atatctcgaa cggagccatg ggaatttatt ataaagatga tgaagcttcg
     2101 gaatggacaa gaatcgatgc aatcagcgac ctggtcggag tacgtttctt gggtagcgat
     2161 aatgctggga atttgtacct ttttcttcgg atttctcaat caaaagatgt tataacaagg
     2221 tattacagat tgagcccgga gcttgtactg acagaactgc taaatatacc cggagcagca
     2281 actggaaccg gaacgcccta taatattatc gcaggaggag caaacgactt atactatttt
     2341 tacacaaatg acgaaatgac agataagtta tatcgttcgc tcgattcagg agagacttgg
     2401 atcgagttaa acatgggcta cccaaattat acttttgttt tcgatttcag aaaaatagac
     2461 ggaagctttg caaatgttgc ttcgcttgta aatgatgcag gagagcttgt gtttattctc
     2521 agaacaaaat ttggtgtgcc tgttacttat gaaaacatac ttctgaagta taatctttcc
     2581 gatgatatgt ttacacagct tgacgcttct ggattcccgc aggatatgtt gtatgcatgc
     2641 tcattaggta agtccgatgg ttttattccc tattgtgtag caactaactt cggatttttc
     2701 tactccgatt ccgatttgga taactggatg cccgattctt ttgagcacgg ttacagaaca
     2761 gggatggttc ccaatggaat tgtcgatctg ggcaatagaa ttattgtttc aactctgtgc
     2821 catggcatat ggtacagcga tttttacaac agcattgttc aagaaggaat aaacaatgtt
     2881 ggagtattcc cgaacccagc ccagaaaact atttcagtta tgggtttaaa cgccggggat
     2941 aaggtaattg tttataacac tcagggagta gaactgatgc aattcaccgc aggtaccagt
     3001 agtcagagtg tgaatgttga gtcttttgag cagggcatgt attttattaa ggttaatcac
     3061 aacaacgtcg aaagaataat gcgatttatt aaagaataga gcgcaattaa aattttttaa
     3121 tgccggtaca aaattccggc attttttttt acttttgaat caacaattat aatagtaatt
     3181 ggttacaaac attatttaat attaaataat ttgcatgaaa aaccatatca gtgtcaaaca
     3241 gggtttggat atacgtcttt atggtgaagc aaacaagatt atacaaaacg aaacagcctc
     3301 ttcttcttat gcacttgttc cggacgactt tcccgggttg acgtcaaagc cggttgcaca
     3361 gcctggcgac acactgaaga taggttctct tgtatttgtt gataagtata aacctgaaat
     3421 caggtttgtt tcacccgttt cggggattct caaggatatt gtacgcggag aaaaacgaaa
     3481 aatcatgaag taccttatca ttcccgatga tacaaatgac accgaagttt ttgaaaaacc
     3541 tgatgtgaac acgctcacaa gagagcagtc agtatcgttt ttgtgtaacg ccggattgtg
     3601 gccgtttgta actcagaggc cttatggagt aatagccaat cccgaaacta ccccaaaatc
     3661 gatatttatt tctttctatg acaccgcacc actcgcaccc gattatgaat tctttctgaa
     3721 agattatttg cagcagatga ttcatggttt aaagataatc cggccattaa ctactggaaa
     3781 aatacatctt ggttttgcga aaaacagcgg acttattcaa tcattccttg gccttgattt
     3841 tgttgaaacc cacacaattt caggtcctca tccggctgga aacgtgggaa caataatcaa
     3901 taaaattgac ccgataaata agggtgaaat agtatttacc ctcatgcctc aggatatcgt
     3961 ttccattggt aatacagcat taagtggtaa atattctccc gaaagaatca tagctgtcac
     4021 aggttcagag cttacgcata caggctatgt gaaaacaagg cacggtgctt caattaaatc
     4081 attgcttaac ggaaatgttg aacatgaccc cgatgcagta agaattgttt cagggaatgt
     4141 acttacaggt actaatgtcg gttataaagg ctatctggga tataagcacc cgcagataac
     4201 agcaattccg gagggaaaaa aatatgtgat gtttggatgg atgaagcccg gatttaaaaa
     4261 attcagcaat tcgcacactt atttctcatg gctgtttccc aagcgcagat accgaataga
     4321 taccaacctg aatggtggat tacgcaattt ggtagttacc ggagaatacg aaaaagtatg
     4381 tcccttcgat atttacccgc aatatctcat taaagctata attgcgaaag atattgacaa
     4441 aatggaacag ttgggaattt atgaggttat cgaagaagac cttgctttgt gcgaatttgt
     4501 ttgcacttcg aaaacccaga ttcaggcaat attacgggaa ggcctcgatc ttatccggtc
     4561 cgaaatgaat taatattatt ctaaagaata ctgtatgaaa tcagtaagaa agttattaga
     4621 taaagctaag cccaattttg agtcaggggg taaactttat tttcttcatt catttttcga
     4681 tgcaatggag acatttttgt tcgtacctga caaagttaca gagaagggag cgcacatacg
     4741 cgatgcaatt gatatgaagc gcaccatgat tttggttgtt gttgctctta tgccagcatt
     4801 tttgtttggc gtttggaata caggttttca attttatttc agccgaggaa ttgatgccgg
     4861 tttctggcag atagtagggt atggattaaa aatggttatt ccattgtatc tggtgtcgta
     4921 cggcgttggc gtaggggtag aggttattgt tgcacaaatc aggaagcacg aagtaaatga
     4981 aggagtattt gttacctcat ttttaattcc tttgttgctg ccggctgata taccgttgtg
     5041 gatgcttgca gttgcaactg cttttgcagt attaattggt aaggaaatat ttgggggtac
     5101 cggtatgaat attatgaatc cggctttatt agcccgggct tttctgtttt ttgcataccc
     5161 agcatggatg tcaggcgata aagtttgggt atcgggttta acacaaggcg aaggagtggt
     5221 cgacggattt acaggggcaa ctcctttggc acaggcggct gctggaagtc cacattttac
     5281 cgacggactt ggaaataccc tttcaacaat ggatttattt attggtgcaa ttccaggatc
     5341 tattggtgag acgtcgaaac tggctatatt attgggagct gctttgttgc tttttgccgg
     5401 aattgcaagc tggagggtca tgttttcgat gctgatagga ggaatgtcgg taggaattct
     5461 gatgaatgtt ttaggtaata cgccttatat gcaaatccct ttttacgacc atttacttat
     5521 tggcagtttt ttatttggga tggtttttat ggcaactgat cctgttactg ctgcccaaac
     5581 aaatacaggc aaatttatat atggattcct tattggagtt atgattatgg taataagagt
     5641 acttaaccct gcttaccctg aaggtacgat gcttgccata cttcttatga atgtatttgc
     5701 accccttatc gatcattacg ttgttcaggc aaatatttcg aaaaggttga agcgtttcaa
     5761 acaaattaaa gcataattta cctatgaata cgaacagcaa taaatacatt tttatctact
     5821 cagttatcat ggtggttgtt gttgcatttg tgattgcttt tattgccaca caactgaagc
     5881 cgcgtcaaca aaaaaatatt gagatagaaa agaaacgaaa tattctctcc accatacatg
     5941 tggaatctac taccgaaaat gccgaacaat tgttcagcaa acatgtcgtt gacatgttta
     6001 gtgtaaatgt tgcaggagaa atcattaagg gaaccaaccc tttatattgc gataaaaagg
     6061 cagaatttac aaaacccgca ccccagagag aactgcccgt gttcgttgcc aaatcagctg
     6121 atgggaacac aaagtacatt atccccttgt tggggaaggg tttatgggga cctatttcgg
     6181 gctacgtatg tctgaacgaa gacatgaaaa cagtgtacgg tgcaatattt atgcacaaat
     6241 ctgaaacccc cgggcttggt gctgaaatat cgacctcgtg gtaccaagca cagtttgacg
     6301 gaaaacaaat ctacgattcc accggcaact ttacttcggt tagagttgtc aagggtggcg
     6361 ctgataaatc ggataaacat gctgttgatg caattacagg cggcaccatc accagcaatg
     6421 gcctaaatga aatgttgttc gacggtctta agcagtacga ttcttatttt aaaaaaatgg
     6481 ctaataaata attactatgg cattatttaa aaatctgcgg ttaatcaccg accccatcaa
     6541 taaaaacaat cctattacag tacaagtgtt gggaatttgc tctgcacttg ctgttactgt
     6601 gaaaatgaaa ccggcatttg taatggcctt aagcgtaata tttgttactg caatgtcgaa
     6661 tgtgattatt tccttactta ggaatacgat cccgaaccgg atacgaatta ttgttcaact
     6721 cgttatagct gcagcattgg taattattgt tgacgaagtg ctgaaatctt tcgtgtttga
     6781 tatcagcaaa caattgagtg tatttgtggg actaattatt acaaactgca tcatcatggg
     6841 ccgactcgaa gcattcgcct tatcgaacaa gccttggcag agttttttag atggtgttgg
     6901 taatggtgcc ggatacggat tgattctgct aattgtttct tttttcagag aattgttggg
     6961 cagtggcacc atctgggatt ataaagtaat acctgatgcc tggtacactg ccggctacat
     7021 gaataacggc ctcatgatat tgcctcctat ggccttaatt gttgtcggaa taataatatg
     7081 ggtgcagcgc ttgagaaata aaaaccttat tgaaaacaaa taaaacacgg ttttatggaa
     7141 aatatattca atattctggt taaaagcata ttcatcgata atatgatatt tgcctatttc
     7201 ttaggcatgt gctcttatct ggcagtttcg aaaactgtga aaacgtcagt tggcctgggg
     7261 gtggcagtta tttttgtcct cctgataacg gttccggtaa attacctcct cgaaatgtat
     7321 gttctccgcg aaggcgctat ggcctggctt agccctgcaa tggctgacgt tgaccttggg
     7381 tttctaaggt ttattctttt tatcgctgta attgcttcaa tggttcagct tgttgaaatg
     7441 gttgttgaga agtttgctcc agcactctat tcatcacttg gaatttttct gcctcttatt
     7501 gcagtgaatt gtgcgattct tggaggagcc ttgtttatgt cggaacgcga ttatgccaat
     7561 attggtgaag cgactgtttt cggactggga agcggtatcg gttggttttt agcaattgtc
     7621 ggtctggcag caattaggga aaaaattaag tattcgcata tcccccaacc acttcaggga
     7681 ttaggtataa catttataat caccggttta atgggtattg cgtttatggg atttatggga
     7741 ataaaactat aatacttgaa ttatgatttt tattatgtca caaaatataa tgatacttgc
     7801 agtaagcatt ttggtttttt tgctgattgt attattactt gtctcaattt tattatatgc
     7861 taaggccaag ctaatgccta aagggaaagt actgattgag attaacggta aaagcacagt
     7921 tgaaacggaa cctggttcaa gcattctggg aactctaggt aataataaaa tactcctccc
     7981 ttcagcatgt ggcggaggag gaacatgtgg aatgtgcaaa tgcagggtga ttgaaggcgg
     8041 aggtagtatt ttgccaaccg aaacgggttt cttctcacgt aaagaacaac agaattactg
     8101 gagattgggt tgtcaggtaa aagttcggca gaatatgaaa atcgaaatcc ctgaagaagt
     8161 aatggggata aagaaatggg agtgcgaagt ggtaagtaac cgtaatgtgg ctacttttat
     8221 taaggaattt gttgtgaaac tgcctgaagg tgaattcctt gattttcgtt ccggtgggta
     8281 tattcagatt gatgttccta aaattgatgt tgattttgca aaagatattg atgtggaaca
     8341 ggaattcagg gatgaatggg ataagttcgg aatgtggagc ctgaaaatga aaaacagcga
     8401 ggaaactttc cgggcatatt caatggcaaa tcaccctgca gaaaataaca tcataatgct
     8461 caatattcgt attgcaaccc caccctggga ccgaaaaaag ggtacattta tgaatgttaa
     8521 tccgggagtg tgttcgtctt ttgttttttc tcgtaaaact ggggataaag tgtttatttc
     8581 gggaccttat ggcgagttct ttattaaaaa cactcagcgc gaaatgatgt ttattggcgg
     8641 tggagcagga atggcaccca tgcggtctca tatttttcac atgttcaaca ccgtgaaaac
     8701 aaaccgaaaa acaactttct ggtatggagc acgatcgaaa agggaaatat tctatgagga
     8761 tgaatttcgt aaaattgaaa acgagtttcc caatttcgct tttaatatag cgttgtcgga
     8821 acctaaggct gaagataatt ggacaggtta tgtgggtttt attcatcagg ttatttacga
     8881 taattatctg aacaaacatc aggagcctga agaaatagaa tactaccttt gtggtccccc
     8941 tctgatgaat gatgctgtcc aaaaaatgct cgataatctt ggcgttccaa aagaaatgat
     9001 tgctttcgat gattttggcg ggtaatttct ggctttatga aaaaccgtat aaacaacatg
     9061 aacctcttac caatactaat actatcggtg tggttgggtg cttgcaccgg tagcaataaa
     9121 aactactaca actgcgaagg tagaacacag ggaactatgt atcatgtgat atatgagcac
     9181 aatgaagaca tcagcgatga aataaattcc ttccttgttg attttagcaa gtcgctatca
     9241 aactacgata gaaactcggt aatatcaaaa tttaattata acgccaagga ttttaaacca
     9301 gactctttat tgattacaat gttaacagct tcgcaagaag tttggcaaaa aactgaaggt
     9361 gctttcgata ttacggtagc ccctgtagcc aatctttggg gctttgggtg ggaaaaacct
     9421 ggaaacattg atacttcgag aattgttgca ttaatgcaat atgtcggtat gaataaaatc
     9481 agtatttccg ggcaacaaat taccaaacaa tatcccgaag ttatgattat tggaaacggt
     9541 atagcacagg gtctttcggt agattgtctg gcttattttc ttgaaagtaa aggaatatcc
     9601 aattatttaa tcgaaatagg aggagaagtg tgtgcaaaag ggtacaaggc aaatagtgaa
     9661 ctttggaaaa ttggcattga taagcccatt aacagcagtt attcgaaccg acagaaccag
     9721 acagtcttaa gcttgaccga ttggtcgctt gcaacatcag gtaattatag aaaatttgta
     9781 gaaatggggc agaaaactta tggacacagt atcaatccaa aaacaggcta tcctgcaaaa
     9841 accgatgtgt taagcgttac agtagctaca aggcaatgca tgtatgccga tgcttacgca
     9901 accgcattta tggtaattgg ctttgaaaaa tcatttgaac ttgcaaaaaa aatacctgac
     9961 ctcgaagctt atttcatata tatagcaccc gatagtacac atcaggttgc ctataccgat
    10021 gggtttgaca aatttcttct cgacgagtaa taccaatata ttatttgaac atttgcccta
    10081 ggtttttgtt tacactaaaa acctatctat atgaataatc tcttgtttga aaaaaacgta
    10141 tttaccggtg atttagctga gtatgataag gttttggaat ttatctcatt ttttgcaaat
    10201 attaatgaag aggactatat tagctataac gaagataaga ctttcgagta caccccccgg
    10261 ttttatgagt ttattgaatc cctgttcagg gctgggcttg ttgaggatta taacaagatg
    10321 agagaatttc tgagcaatgc gtgtttcgat tttgaagaat ccgatgaata tgtttcctgg
    10381 atacacgaaa tgaataaaat tatttgtagg ccagagttat tgagtaaggc aaatttgtcg
    10441 ttcatcagaa aagcttttct tacgctcata aggcttgaat atagaattcc tgggagttgg
    10501 ggtattgata ttgaagtttg tacctggctc aaactcctga aaagacttaa ccaaatacga
    10561 cctgaattag cctgaattct gccaaaacga caggcgaaat ataaaatatg ttggcagttt
    10621 ttcgccttta ccgacattat ttcctgataa atattttaat ttcctgatgg cacagcaatt
    10681 gactgttaac atcagaaatt aaatagaaat ttaaaataaa atacaatggg aaaaataatt
    10741 ggaattgatt taggaacaac aaactcctgc gtagctgtga tggaaggcaa tgagcctgta
    10801 gttattgcca atagtgaagg aaaacgcact accccttcgg tggttgcgtt tgttgataac
    10861 ggtgaaagaa aaataggcga cccagcaaaa aggcaagcaa taaccaattc tcaaaaaacc
    10921 atatactcaa tcaaacgctt catgggagaa cgcttcaata atttgtcgaa tgaggtttcg
    10981 cgcgttcctt ataaagtagt cgccggtgat aataacactc cacgaattgt tattgatgac
    11041 cggaaattta caccgcagga aatatcagct atggttcttc agaaaatgaa gaaaactgcc
    11101 gaggactatc tggggcagga agtaacagag gctgtaatta ctgtacctgc ctattttaac
    11161 gattcgcaac gtcaggccac aaaagaagca ggtgaaattg caggactcag ggttgccaga
    11221 ataatcaatg aacctacagc tgctgcattg gcatatggtc ttgataaaaa gaacagggac
    11281 atgaaagttg ccgttttcga ccttgggggc ggaactttcg atatatcgat actggaattg
    11341 ggtgatggtg ttttcgaagt taaatccacc aacggagata cccacctcgg gggtgatgat
    11401 ttcgacaata tggtaattga ttggcttgct gcagagttca aaaacgacga gggtgttgac
    11461 cttcgcaaag atccgatggc aacacaacgc ctgaaagagg ctgctgaaaa agcaaaaatt
    11521 gagctgtcaa gttcagttac aaccgaaata aacctcccgt atattatgcc cgtcgacggt
    11581 attccaaagc atttggtaaa aacacttacc agggcaaaat ttgagcaact tgtcgataaa
    11641 cttattaatg caacgattga gccttgccgg aaagccctca aagactcagg atattcggca
    11701 tccgaaattg acgaagttat tcttgttggt ggttctacac gtattccagc agtacagaaa
    11761 aaagtggaag acttctttgg aaaaacacct tcgaaaggtg ttaaccccga tgaggttgtg
    11821 gcagtaggag ctgcaataca aggaggtgta ctcacagggg aggttaagga tgtgcttctt
    11881 ctcgatgtta ccccgctttc gttgggtatt gaaaccttgg gtggagtgat gacaaaactt
    11941 attgaatcca acacaactat cccaacaaaa aagacagaaa ctttcacaac cgctgccgac
    12001 aaccaaccct ctgttgaaat tcatgtattg cagggcgaac gttcgatggc aagtgataat
    12061 aaaactatcg gacggtttca tcttgacggg ttacctccag ccatgagagg aactcctcag
    12121 attgaagtaa ctttcgatat cgatgccaac ggaattctga aagtttcggc aaaagataag
    12181 ggtaccggca aagaacaaag cattcgtatt gaagcctcat cgggattaag cgatgatgaa
    12241 gttaaaagaa tgaaagctga agctgaggca aatgccgaaa tggatagaaa agccaaagaa
    12301 cgaatcgata aaatcaatca ggctgacagc atgattttcc aaactgagaa acagctgaaa
    12361 gaatttggcg ataaaatacc tgaagcaaaa cgtaaaccta tcgacagtgc tctcgatcaa
    12421 ttgaaagaag ctcacaaaaa tcaggacgtt caatcaattg ataaggccat tgccgatttg
    12481 aacgctgcgt ttcaggctgc ttccgaagat atgtataaag cacagaacca accacaggga
    12541 gacccaacac aacaacagtc cggctcatcg cagaacacgg gcaactccaa gccggaacag
    12601 gaagtaactg atgttgattt cgaggaagtc aaataataaa aagcaaacaa tataaaagcg
    12661 caggatgatt tcctgcgctt tttgtttagc tagggtcgat ttcattttgt tcgaatagct
    12721 ttcccagaat ccgcctgtcc tttttattgg gacgcgtttg aggcttgctg tttcgaactg
    12781 aacgggcaat gcgcagtcgt tcgagttcag ctgtgggggt tgtttcggag atataatcag
    12841 atactaacgc attgctgcat cgtttatcat taatctgaat aacagtaaaa gtgcgaaaaa
    12901 ttgggtttat tctgacctca attttatctc cctgttttat aatgtatgaa ggcttaacgc
    12961 ggttgtcatt aacaataaca gacccatttc ggcatgcggt tgttgcttca tttctggttt
    13021 tatacaacct tacagaaaat aggtacttat caacacggac attcatcgta tgcgaataat
    13081 tatatttttc aacaaatatt actatttttc gtaataatta ttattttcgt aaaataactc
    13141 aaattaatta acctacatat gaaaaacatt tacattttca ttattacgct gattatggca
    13201 atgtacggaa gcattgcatt tgcacagaat cagaaaatga tcggtgaaat tcaggacagc
    13261 gatgaaaaaa ttattcctgg agcattcgat gaggaactct tttcaaaagt cctgattaaa
    13321 agactgaatc acttgatgga agattcattg tttctcgaag gatatgtttt gcatgaattt
    13381 tttaaagctc ccgcagagga acatgcaaaa attatggcgg aggatggaag tgctagcatt
    13441 gaaggtaaag gagagtatgc aacaatcaaa tccaggcttg aactggcagg cggatccggc
    13501 agcggttttg agctggttgc caaatcgagc ataaggaatg gagacgcaaa cttcacttac
    13561 aatcagcttg cagatttttg cataaaccgt tggcttacga gcagtaaaac aagagaacct
    13621 ttgttgcgtc aacagtattt ctttgctggg ataggtgcaa aatttgacac tgattctaca
    13681 aaaatttaca tttccatgta tgtaggcaac tgggaatcgt tcctgcaagg ttctgaaaga
    13741 tacgatgaac tgccatttca ggtgtcgaag aaaaaatttg ggttgaaact tccggaaccg
    13801 gaaatgaatc gcaaaatgct ttcaaaagca aaacgacgct tccccaatct gctggagcta
    13861 cagagtggat tatcggtgaa tgaaaatggt gaaatatatt tcaagtattc cgatttgcgc
    13921 ggtttcaagc gcataatgcg caactcaaaa gatggactgg cggttgatat tgtgcaaact
    13981 tgccagttcg ataattgtgg tgaaacaaac atagttgatt ttggcaggag aagccggggt
    14041 attatgctta aacctgtact ctcaaaaaaa atgtttaaaa ataatattgc cccacccgaa
    14101 ggtactggaa aacggaaaaa agttaagaaa cttgaggtgt atatggggca gatgccggaa
    14161 ggcctgaatc cggatgatgc ggaattgaat cttgtattta tccaaaataa atacattgtt
    14221 acgaatatcc acccaaccta tatcgataag gcaatataca attatgccca aaaaattgat
    14281 ttgttgccgg atacgctttt acctgatggg atttctgaat attttccttc agcaacatca
    14341 accaaattgt catttcgtat tcctttcgag caaggtaagt tcaactacga ctccgacgac
    14401 atgataccgg tgttaacagc cttgaatgaa cctgcgttta ttataaataa aattcatatt
    14461 gcggcctatt cttcactcga aggtagcgag gccgagaatg ctgcattgca gaaaaaacgt
    14521 gcaaacagta ttgtttcagc tctggaagaa aaccagaatg ccagcattat tgactcaatt
    14581 accacggcac ccaattttga acaactgaaa acagatgtta agggaactcc ttttgaaaaa
    14641 gttgccgaaa tgaactataa agaagccatt caatatgtaa atgcgcatgc cggagaaatg
    14701 gaatatttgc taaaaaacca caggtatgct gacgtaacca tttgggtaac ctacgatgtg
    14761 gaaggtgata aagaacaggc ttatgttctc gaccaattca acaaagaagt agcagccgag
    14821 agatacgata aagctctggc tattcagaaa tacattttta aacgtgttgt tgaaggacga
    14881 tataattcaa aagcagtgag tggcatggta attccaaaca taaaaccttg tgttgggctg
    14941 aatatgaata aaatttgtct cgaaaggatt gttgccaatg aacctgttga ctcaacttat
    15001 ctagagagaa tcgaagattt acagacaatg gatggcgaga atatttatgt taggtacaac
    15061 gatatttatt gcgaggtaat gctcagcaat atggataaca caaacgtggt tgaggagctg
    15121 cagggaagaa ttgacaatct gtattccata tttccgaata aaatgacagt tgatttgctg
    15181 aacattgaac tccagtatca gattatggaa gtgtataaag actcattggg tttcgataat
    15241 ccggtagtaa ttgcaggttt gaataaaatt aaacagattg tgggagcaac ggcaattaac
    15301 tgggaaaacg ctcttaagat ggctggtata tttattaacc attacgatta tgaatatgcc
    15361 tatcagttgc tgtggccgtg ggtagacaaa gatcctgtca acacttcttt gttgtactct
    15421 tatgtgtcgc ttgcctcaaa agttgaatat cgttgctata gtaatagatt tttaatggca
    15481 atggaaaaac tcaaacaaca tgaccctaaa ggtttttgca aactcttcag gggggataaa
    15541 ttctcggttc aggtatatgc caacgaaaaa ctgaaggaat tgtattgtaa ttcctgtaaa
    15601 caatagttta atacaccgga agctcccaag ggcttccggt tttcattttt ttattaccca
    15661 tgaagtcgat tttactattt cttctgcttt ctattctatt gggaaattgt aatgcacaag
    15721 atttttttgt aaagctcagc attcctgatt acccggagag agaattatct ttctgttcac
    15781 agtttggcga taaaagcaga attattgaga catttcgcac cgataaaaat ggctatgtca
    15841 gctttaatac tactgaccta gaaataggtg tttatcgtgt gtattttgat tcggaagttt
    15901 actttgacat tataataaat aaggagaata ttagcttgtc gttgctagcc aacgcacccc
    15961 ttgaatcgat ggaagtaatt gagtcaatcg aaaatagggt gctctataat ttccttaaac
    16021 agaataatgc cattaatcaa aaactagatg tgttggaata tttccttgcc aaatatgcga
    16081 aggggaaact atataagcgt gttacaaaag agtaccaaga ggtgataaaa gaacaggctt
    16141 atttggccga taatgtgacg gcaggtgaaa agaatacttt tgcagcaggc atgattcaat
    16201 actataaaac tcccattata cccaaggagg tcgcacaaaa tggaaatgaa aatatgtttc
    16261 tcagagagaa attttttacc tatttccctt tcgacgatac attgctggtt aattcgtcgg
    16321 tttattcatc agcagtgctt aactatttga aattgtttgc agtatcgggc gattatggtg
    16381 ccagtgtgca gggttttaaa tctgcttctg agagaatttt gaattatacc cgtccaagag
    16441 aagcaatctt ttcacatgtt ctgaaaattc ttgttgatgg ttttgaatcg ttgcagctct
    16501 tcgatatcgc cgactattta ctcgaaaatt acggtagcaa gtgcgttgac gatgacaatc
    16561 tgtcgttacg ctataaaaac atcaccgagc tgccattaca gtcgaaggct cctgttgttg
    16621 aatacatcga taaagcaggg aatattacag attcttacga aaacaaaaat acaataattg
    16681 ttttttgggc gacgtggtgt gatcattgta ggcaggttat tccggagtta tccgagtatt
    16741 ttgaaacgaa ccacagcaat agcatttcag tagtgcttat ttcgctggat accgataaaa
    16801 gcacacttga caattttgtt agagagaata gtattgattt gcctgttatt tgcgattaca
    16861 gaggttggga gggaacttac gtcgaattat ttgccattta tgcaacgcct tttttctatg
    16921 tagttggacc ggatggagca attgtttcga agcctcatga ttataatgag ctattaaatg
    16981 ttctaaaatc cctgaaatga ccccgctaat caaatcaata agttttgtaa aatcgtcgcc
    17041 tgattacaaa agttgtccct cccccgaaaa gcatgaatat gcttttgccg gaagatcgaa
    17101 tgtgggaaaa tcgtcgctga taaatatgct tgcgcgtcat aaaggcttgg cgaaaacctc
    17161 aggagtgccc ggaaaaacgc aattgataaa tcattattta attgatgata gctggtattt
    17221 ggttgatctg cccggttatg gctatgcgaa aacatcaaaa aagaacagag agctatttca
    17281 aaaaatgctg caaggctatc ttctgaaccg aaataatctt gtatcggtgt ttgctttaat
    17341 cgattgcaga atcccaccgc aaaatagcga tataagtttt atgaatttta tggttaccaa
    17401 tggagttcct tttgctcttg tttttacaaa aaccgataag ctgggtccct tggtgctcgg
    17461 ccgcaatatc gagcattaca aacatacctt gcttgaaatt tgggaagatc tgcctccaat
    17521 ttttatcaca tcgtcagaga aatcaacagg cggtgatgaa ttaatcgatt atatcaatac
    17581 gttaaaccaa cagatacaat taaaaaagag gccataagcc tcttttttaa tttcttatgc
    17641 ttgctatcca ttaaaagaaa cttcttcctc ttttaatggg ttcaccaaca ttaactccaa
    17701 atacaatttc ttgtgatccg atagtatagt tcgagaggtc gctgaaggta aagtcatagg
    17761 agtacccaag ataatacctg tttatcttca ggccgattgc agcaataaaa gcgtcgttcg
    17821 ggcgaatcga catcccaaac caataatcat cgttatagta aagttttacg ttaaagtcaa
    17881 gctgaattgg tgtaacttct gtcattttca tcatcaatgc aggttcaatt ttaaatgagg
    17941 gtgcactttg gaaatcgaat ttatatcctc ccatgatata atagtgccga accatcgtgt
    18001 tatcgtccag atttctgtta atctttaatt tcgactgaaa caaatgtgca gctgtgatac
    18061 ctgcatagta tttttttcca taaaaataca cgccgaatgt tgcatccgga acaatagtgc
    18121 gttcgatcat ataggtaatg gatggatcat catagattgt caattgaaaa aaccgctggt
    18181 ccatcttata ctgaaatgca atcgctgcta acccaagtgc aactctattt aacctgttaa
    18241 aagcaaaatg gtaggaatat accgcctgaa tacctgtatg acttaccggc ccaaaactgt
    18301 cattaaaaat gaaacctcca ataccgacag ttttattctg aagtaaagtg tgcccgctaa
    18361 tcgactgtgt agatggagag tcgttgatac caacccactg tttgtgaaca ctcagtcgta
    18421 ccggtgaata tttttcgctg cctgcaacag cagggtttat cacaaaattg ttcaggaagt
    18481 attggctgta aagcggtaac tgctgagcaa atccggcaat gggaagtaaa ataactgtaa
    18541 atacaactcc tagtagaaat cttttcataa atttcaaatt aaaatgctat tagaggctat
    18601 aaaatctcat aatcccaaat ataaacaaaa tttgttcata ataaacaaat tattaacgat
    18661 taaaagtttc aaacctaata aaaatttttt atcgatgtac ggaaataccc ttatttactt
    18721 aacaatcaga taaaaaagag attgttacaa atagtgttaa gtgatgaaga aaaaccggta
    18781 tagcatcttg aaatgttaat aaaatctgga gctcttgtag ctcttatccc caatattcca
    18841 tccaaaagtt aactcttcag agccgattgt gaaattggag agttccgaaa aagtaaaatc
    18901 atgcgaaaaa gcaaaataat atcctttata attcatgcca atcataaaaa taaatgaatc
    18961 tccgggtcga atcgacatgc cagcccaata gtcttcattg tatatcattc ttatgttaag
    19021 gtcaatttgt attggggtag tttctgtgaa tttgattagg gaagatggct caatctgaag
    19081 gctttttgct gaagggaaag aaaaacggta accgccattt agaaaatagt gccttaccat
    19141 tttatttgat ttttcatcca tataagtact cgtaattttt aaatttgatt gaaaaatatg
    19201 agcaaccgat aatccagccc accatttttt tgaatacgca taagcaccaa gattcccatc
    19261 gggaacaaga atgctcatac ggtttcggag gagaaccggt tcaaaggaat gaaaatcttc
    19321 ctgatcgagg cttataatat attgcattaa tatggctgac accccaaatg ctactttaat
    19381 gcctcttgtt gctcttaaat ggtaagcata ggtaaattga gatccaatag ttcgcaccgg
    19441 gccaaagcta tcgtgaaaaa tcatgccacc aatacccatt tctttgtttg atagcaaatg
    19501 gtggcaacta attacctgtg ttgatggggt ttggtcgatg ccaacccatt gcctgtgtat
    19561 tgagagcctc agaggagaat aatccttatt actgcccgcc gctgcagggt taatcac
//
LOCUS       DAOOQP010000048        19235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3463_length_19235_cov_7.001460, whole genome shotgun sequence.
ACCESSION   DAOOQP010000048 DAOOQP010000000
VERSION     DAOOQP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_19235_cov_7.001460"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   53..2253
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=53..99
                     /rpt_unit_seq="ggtgtttatcctatttcaaagatacgaatttgaaagcaaatca
                     caac"
     assembly_gap    2620..2719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2795..4030)
                     /locus_tag="PLK75_06690"
     CDS             complement(2795..4030)
                     /locus_tag="PLK75_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38971.1"
                     /translation="MQEEQKVPKWIKKLQESSWELELLISGGAIFSLFKVGGIIPGFF
                     VSISKYASVPGLSIFMIFVMIALKSLTLGFVVHLAMRSYWLALVCVSYVFPGGLRQEK
                     IGWRKPFRIPAEGETGMYKQIMSVDRFCGLIIYFSIILAVILAGFILFFITSFTIPVI
                     TLPVDKAVYFTVFYVVFVVYLFDFITFGLLRKIPYLSYIVYLPFRLIDFLTLRFLYQR
                     SLLLLSTNIRKMKIALILFAYMLAAISFSYVSLYRTMHWPNILDQRTYRFQENMGSNI
                     YIDNNYYLDDQDGTKVKDIAIQSLIVKDKCLKVFAVYDRDWDKLMDYISGKDGEKHFS
                     DLALVRIDDSVYNNIEWIPTRSRDLNRFGMTAFIPITQLGMGRHVLTITANLDSVQMK
                     NLPDGYRIKEAEIVFWKDY"
     gene            complement(4088..6514)
                     /locus_tag="PLK75_06695"
     CDS             complement(4088..6514)
                     /locus_tag="PLK75_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38972.1"
                     /translation="MKVFIVSLFLLIGSQLVAQNFTIDEQPMSRPLSAREQSELSTLP
                     ELKLPESYKNKSLPVEVDNSTLPYHCGLFEQSGYECGQAASVANAFTYEIDRLRGLDA
                     TLTENKYPTYFAWNWENGGYGWYGASYYHTLKLLKTVGIPNVSTYGGAYDSGGSSRFM
                     TGYGNYYAGMHNRISGAAAIKCDTEDGILTVKNWLNDHLDGSETGGIGFFYSQYQGAT
                     HYLPDESPHAFENVIISWGDSPDHAMSIIGYNDSVRYDYNGDGQYTNDIDITGDDIVD
                     VCDWEIGAFKMVNNYDVPYYAWMMYRTLAMESDNGGVWNHTVNVMYAMSEYSPVLTYK
                     VNLYYSNRGRIKILTGFSTDLDATSPDYYLSFPIFDYQGGELPMQGSIGDEYKYMEFG
                     LDVTPMLNLINSGTPVKFFFMVIENDNWGWGNGRIISFSVMDYSAGEPIEVVSSQSDV
                     SITNNNLTTVSLVHTPVFEKPQIAPDVLPEPVLNQNYSQQLESAGGTPPYRWEFNIDY
                     HETEIVETAPNATTPIIDTYIELPFEFDFYGSTYQGFYLSSNGYIGFSNDTYDLPYND
                     NSTNKLTVSFMHRKCIAAFMSSVNCSTFYSEGTDYCLINWTGTDINTTLKLEANGTIT
                     VYYNDCKGLNGTVWISGVSNGNLENYTLSPLSGLPGDVPVTAYSFHASMIPQELQLTE
                     SGLLSGTVTTEELNSNLCIKVTDSRGLTDMNNYQLFSAIPSESYNNSAVIVISPNPAG
                     DVINAYIPGQPDQAAQAEIFNISGSKVCEKSIHTITDNTIEITDLQPGQYTLKFSCGE
                     KVFIKKFIKL"
     gene            6779..7672
                     /locus_tag="PLK75_06700"
     CDS             6779..7672
                     /locus_tag="PLK75_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY38973.1"
                     /translation="MKRLTQILAIAFVSIATPCTAFAQNNFIVCGIPDLDRDTYNDIN
                     DTTIQPYHVKSGSWYNRYNFDMDGNGGLDVKIRTTEWENAGGGGNNTQLLYLTNNIKF
                     AFGEQLVDTIYYFNPPQIQYYNFPKTFNYNDTIDSRYNFLQSDMFLAVIGGPGFYFIY
                     HPGWLNLGEKYIGISMEKNDVMLYGYILVEVTGYSRVIIKEFAMNINPYVGIEPGKTN
                     QVFEMYPNPASNEVFIKQSNQNTGNPAVFSLYDISGKMVFQATSVPEDGHIALPALAK
                     GLYVAEFEVDGQRYSKKLAIE"
     gene            complement(7687..8776)
                     /gene="prfB"
                     /locus_tag="PLK75_06705"
     CDS             complement(join(7687..8721,8723..8776))
                     /gene="prfB"
                     /locus_tag="PLK75_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113942210.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOY38974.1"
                     /translation="MVNTEQQKDINGRLAALRGFFDLDNKIARVNEETEKTQAPGFWD
                     DPKAAELHMKNISSIKQWIDAYNDVKNLVDDFQVMIDFAETGDAGEDDVLHAYNQALT
                     AVENLELKNMLRSEEDAMDCVMNINAGAGGTESNDWADMLMRMYIRWGEENGYKVREI
                     DFQPGDTVGVKSVSLEFAGKFAYGYLKSENGVHRLVRLSPFDAAHKRHTSFASVFVYP
                     MVDENIEIEINQADIEWDTYRSSGAGGQNVNKVETGVRLRHLPSGIVIENTETRSQLK
                     NRENAMRLLKSRLYELELRKQLEEKQKIEGQKKKIEWGSQIRSYVMHPYKMVKDLRTG
                     HETGNVQAVMDGRLNDFIKAYLMEFGGE"
     gene            8879..9874
                     /locus_tag="PLK75_06710"
     CDS             8879..9874
                     /locus_tag="PLK75_06710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOY38975.1"
                     /translation="MNLFTPVPVPPPGFNIDYSDTIFSIGSCFAENISLKLAEAGFRI
                     CSNPSGVLFNPVSTGNCILDLIDKKKFSAEALEYDGALYFHNGFHGSFSSPDKEKCLE
                     KIHESMMQASENFTKASILIVTFGTSWVYMHRKSGAIVANCHKQPAETFLRKRLEPGE
                     IFSFWQEILHSLNALNPNLKIIFSISPVRHFKDGAFENQLSKAGLFIAVNQLLESNKN
                     CFYFPAYEVMMDELRDYRFYSSDMLHPSETAIEYIWEKFGEAYFSENTHKLTQKATAI
                     SRSLKHRPHHSNSTAYAEFVKKLKQEVYVFEKENPGADFSFSKKWLSGKNSPESD"
     gene            complement(9871..10983)
                     /locus_tag="PLK75_06715"
     CDS             complement(9871..10983)
                     /locus_tag="PLK75_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOY38976.1"
                     /translation="MTTTYVKELLARSAETDFLSVDEALNLYYHAPLPALMNSANMLR
                     NSMNTPGNVTWIVDRNVNITNICQVQCLFCNFCTKKGSSNAYTLTLNDYHERIETLFR
                     EGGNQLLLQGGLNPELGLRFYTHLFASLKDKYPNLKLHALGPPEIVFLSKKEGIGIAD
                     TLKILIEAGLDSLPGAGAEILSDRVRKQLSPAKCSVDEWLEVMRQAHKIGITTTATMM
                     FGHIETIEERIQHLYLIRELQNEKPPQSMGFIAFIPWPCSFNNTRLLKHYPDLKPISA
                     SEYVRFISLSRIFLRNIQHIQASWLTVGTEVGALCLHAGADDLGSVMIEENVVKSTGL
                     ENTLIAAQMKQLIRQNGFIPVQRDQNYNAVAVTTEN"
     gene            complement(10993..11694)
                     /locus_tag="PLK75_06720"
     CDS             complement(10993..11694)
                     /locus_tag="PLK75_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY38977.1"
                     /translation="MTSHFEEPLCKDCLITNRGIFRKLKPEELDYVSERKTCSAYKKG
                     TAIYKEGQRISGIYCIHSGVIKIYKTGVAGKEQIIKFAKPGDIFGYRSIMNGEPACTS
                     VKVHDDAVLCHIIASDLYALLRKNNDFAIDLLQLACAELGEANNFITDMAQKNVRERL
                     AEMLMLIKNEFGTDTENILKITLTREELANLIGTATESVIRLLSEFKSENMIELKGRR
                     IKILDENKLRKTGNI"
     gene            11890..12900
                     /locus_tag="PLK75_06725"
     CDS             11890..12900
                     /locus_tag="PLK75_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOY38978.1"
                     /translation="MSDKENQFSFDTRNLFNFILAKKKPLTVIVGSALIVSLILSFLV
                     PVKYKSSVILYPASPGSVSQSLLSESFAEKELLKFGEQEEVEQMMQILQSSAIRDRII
                     QKYDLFKHYNIDKAGKYPNTSLYRQYQRNVKVVRTEYMSVRVDVYDENPKMAAAIAND
                     IAALYDSTVNIMQKERTLKAFLLVEQEYNLVKEKIQRIQDTIRTLHEKGVFDFESQSE
                     VFNDQLALALAQGNQRGVQEIEKRLDILAEYGSQYTNLRDQLQEEIKNLAKIESKYAE
                     AKVDLHQDLPHKYVVSKGEVPEKKAYPIRWAIVVVSTFAAFFFALIILLFLDPIKKKK
                     NT"
     gene            12954..13898
                     /locus_tag="PLK75_06730"
     CDS             12954..13898
                     /locus_tag="PLK75_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38979.1"
                     /translation="MEQYFRTKKLFEISLKYKWHLLTIAVVAAAVGVIVSSPWVIKPK
                     YKSFAVVYPANVSPFSEESETEQLLEVIQSLDIRFRVCETFQLGEHYGISKNNPHFQT
                     LLNKEFENNVTFQKTPNEAVQITALDTDAQLASDIVDSILSFYSQKMLTMIRSKSKEI
                     VLIKQGQMNRKEKEIDSLTNIINEYRQKNNLLDYKTQVKVYSEAVSGGKSLEEARKML
                     DNWKQLGSEYMKTDSTLWFAITDFHRLKKDYDEAFMHSIKELTFTHIITHPFPADKKS
                     YPIRWLFVLFSVIGSLFASFLIIAIIEGRKEKTKNGAV"
     gene            13888..15339
                     /locus_tag="PLK75_06735"
     CDS             13888..15339
                     /locus_tag="PLK75_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY38980.1"
                     /translation="MLSKRKIIAFYVLSILFIVLNVLFVYFTESLVFNALPFILLFFL
                     FALFSLDKMLIVSFALVPLSIPLKELLPGLDYDMSLPTEPLLFLILLIFILKQLRDRD
                     FDTAVSKHIISKVLYFYLGWMAITVITSTMPIVSLKFLLVKLWFIIPFYFLLTQVFKD
                     YKNINKSFWFYIIPFIIVIAYTLFRHSAFYFDQKSANYVMTPFFNDHTSYGALLAMYI
                     PILVGMITDKSQKKAVRLISVSVLAIFVLAIIFSYTRASWVGLIAALGLWFLLKMRIK
                     IRTLFIGVGIVLTLFVLYQSQIMMSLEQNKQDSSTNLEEHVKSISNIATDASNLERIN
                     RWNCAVRMFKEKPLFGWGPGTYQFKYAPFQFSYEKTIISTNAGDMGNAHSEYLGPLAE
                     SGVFGLLSMIAIVIVFYITSINLYYKLTDFKTKTLVISIICGLTTYFIHGLMNNFLDT
                     DKASAPFWAFFAIIVAIDVYHKPKSDTEIQTEK"
     gene            15408..>19235
                     /locus_tag="PLK75_06740"
     CDS             15408..>19235
                     /locus_tag="PLK75_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOY38981.1"
                     /translation="MPKAIISFLILWSISLPLYSQINSYPYNEGFDVASIPSEWTQEF
                     VSGAVLWSIGSGGYGGYPSAPYSGSGNALFSSPNYNSDETMLISPEFDLSAVQNPRLR
                     FYHAQYNWDTDQDVMSVYYRQGESGEWILISSYNTAVTAWTLRVIELPEPSSSYFIGF
                     KATSGYGFGVVLDDVVVDDAPQCNTPSEFSVLSTSTESANFNWTSEANLWQLEYGPVG
                     FSHGGGILVENITSAPFTLFGLTEGQSYQAYVRAVCSGNNSEWAGPVSFTADCNLINA
                     NNFIEGFESSSAPPACWSVVYQNTTPPAGNLVTHTTTYAFSGNRSFRFSSYSPGPPYG
                     QYLITPEIGSYTGTRELRFWYKRYSSGSETFRVGYSTTGTNLTTDFNWTEEITDATAQ
                     WKLYNMVLPPNVKFVAINYRTVFQYYLYLDQFQIRIPPTCPQPENLHVSNITAVSAHI
                     NWLAGSNEESWQVEYGFSGFSHGEGTITNTSFPVHILQNLSPNSNYDVYVRALCDEEN
                     STWVGPLSFSTPYGCQQITNISISNVSATSAFIDWVPSGSEESWNMEYGISGFTPGTG
                     TIISGITESQYSLTDLLPNTTYDVRIQAYCGNVYGVSLWSNFSTFLTLPCNDGCNYTL
                     QQSDAWGDGWGETYISVLQNGIETNKVYMTSGSFQSADIYLCNEASVSLVIHPSSFVS
                     EVGLNLLDAFGNSIYQLPISTLEEGISVTTLVTFFASCTEPECYPPINPAITSIHAEG
                     AVVSWEASASIGNYQVSYGFSGFNTNEGYLSEDLTETNYTISGLFPQTTYEVYIRTLC
                     TSGNSIWSGPYSFTTTSMNLSNPIACNAALNIPDNSCVSYSISVNDPENETLGMDVLI
                     DEIRLIMTHNFDGDIDLSLSSPDGTTVLLFSDVGGSGDNFGNNNGSCNQYTSLAMDGA
                     SGAITSGTAPFIGSFIPEGNINAFHNGGNINGDWTLQICDDNAAFTGTLQYFEIVFTE
                     QKYLEWSSLQFVESPENNGSISNSVNLGLYNETFAVSGPLNEGAHYLTSNIPSGLTAH
                     VEVVSPNSALLTLTGYALNHSNASDINNISIEFLNAAFSGGQASEIVNYKQLSLSIDF
                     KNLTDIANLTSSEDEYVCETELNNYFVQYMFVNTGENIIPAGTTFQMRVEDASGNILL
                     SENIILSNNLDLGEDLGGISVNSIHFADIGLHSVKIIVKAPSDILPINDTIVKNIVGV
                     EQNIVFWFAENDSLNVTEYPAIVAAWMDFNPDSSLNYTYYWENGISTSNSIDVIADGW
                     YHCTITSEACPITD"
BASE COUNT         5775 a         3902 c         3683 g         5775 t
ORIGIN      
        1 gaatttgaaa gcaaatcaca acgcattagc agaacaatac gaaagttggc ttggtgttta
       61 tcctatttca aagatacgaa tttgaaagca aatcacaaca aaattttgct gaacgtattt
      121 atgacgcctg gtgtttatcc tatttcaaag atacgaattt gaaagcaaat cacaactaga
      181 agcagggtgt aaatatgtcg aaggttggtg tttatcctat ttcaaagata cgaatttgaa
      241 agcaaatcac aaccactact tcatcatgga tataattgtc cttggtgttt atcctatttc
      301 aaagatacga atttgaaagc aaatcacaac aggtattaca gcagtaactt attaagcgat
      361 ggtgtttatc ctatttcaaa gatacgaatt tgaaagcaaa tcacaacaaa actacgtgct
      421 gatcagatta ttatgaggtg tttatcctat ttcaaagata cgaatttgaa agcaaatcac
      481 aaccggtgga cttatgtcgt ttgcggtgga cttggtgttt atcctatttc aaagatacga
      541 atttgaaagc aaatcacaac tgttcttgct ttgctatttg cgcaagtgcc ggtgtttatc
      601 ctatttcaaa gatacgaatt tgaaagcaaa tcacaactaa gtcattatgt tttgaaattg
      661 atttactggt gtttatccta tttcaaagat acgaatttga aagcaaatca caacaaaact
      721 gggtcgatga tactttttat agacggtgtt tatcctattt caaagatacg aatttgaaag
      781 caaatcacaa caaacggaag cacgttactt tcgataacga ggtgtttatc ctatttcaaa
      841 gatacgaatt tgaaagcaaa tcacaaccgg cctttaattc tactgttgct tctgaatggt
      901 gtttatccta tttcaaagat acgaatttga aagcaaatca caaccaataa tttcacagta
      961 atattttgga tggaggtgtt tatcctattt caaagatacg aatttgaaag caaatcacaa
     1021 caattctgta agtcttttat cccatctctt tggtgtttat cctatttcaa agatacgaat
     1081 ttgaaagcaa atcacaacag tcggcattta cattaacttc ggcacccggg tgtttatcct
     1141 atttcaaaga tacgaatttg aaagcaaatc acaacgggcc tgtcctggct taaaaataaa
     1201 ccgccggtgt ttatcctatt tcaaagatac gaatttgaaa gcaaatcaca acatacttca
     1261 gattgtccag cctgatatcg tcggtgttta tcctatttca aagatacgaa tttgaaagca
     1321 aatcacaact gttttagtgt atgaattata tttgttttgg tgtttatcct atttcaaaga
     1381 tacgaatttg aaagcaaatc acaacgtggt ttcgaatggt ttataaagaa cccaaggtgt
     1441 ttatcctatt tcaaagatac gaatttgaaa gcaaatcaca acagtcggca tttacattaa
     1501 cttcggcacc cgggtgttta tcctatttca aagatacgaa tttgaaagca aatcacaacg
     1561 ggcctgtcct ggcttaaaaa taaaccgccg gtgtttatcc tatttcaaag atacgaattt
     1621 gaaagcaaat cacaacaatg gagcttatga agagttatgc cccgtttggt gtttatccta
     1681 tttcaaagat acgaatttga aagcaaatca caacactcgc gtgtgtagtc atctttagaa
     1741 ttaaggtgtt tatcctattt caaagatacg aatttgaaag caaatcacaa ctaacgacgt
     1801 tggcaaaatc ggcagtgccg tggtgtttat cctatttcaa agatacgaat ttgaaagcaa
     1861 atcacaacgg ggaggtacga cagcatgttg caaacctcgg tgtttatcct atttcaaaga
     1921 tacgaatttg aaagcaaatc acaacttcag gtgcgtgctc cttgggaaga tcctaggtgt
     1981 ttatcctatt tcaaagatac gaatttgaaa gcaaatcaca acaatggatg gtttgaccag
     2041 ggggcagaat gaggtgttta tcctatttca aagatacgaa tttgaaagca aatcacaacc
     2101 gcttcctcgt tgccttcgag tgtttgcaag gtgtttatcc tatttcaaag atacgaattt
     2161 gaaagcaaat cacaacagtg cgatttcgac atcgttacgt tgcctgggtg tttatcctat
     2221 ttcaaagata cgaatttgaa agcaaatcac aacctgagaa atcggcggca tcgatagcaa
     2281 tgggtgttta tcctatttca aagatacgaa tttgaaaccg acaaaggagg ttcgcgagta
     2341 cctataaaaa taaatatttc ttacgagcaa agcaaatcac aacttctatt ctttcgtttt
     2401 tattattttc tactttataa tttccaaagt ccaaaacttt gcaaatcaat ttcgatacga
     2461 atttgaaacc gacaaaggag gttcgcgagt acctataaaa ataaatattt cttacgagca
     2521 aagcaaatca caacttctat tctttcgttt ttattatttt ctactttata atttccaaag
     2581 tccaaaactt tggaaatcaa tttcgatacg aatttgaaan nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnt ttataatttc caaagtccaa aactttggaa atcaatttcg
     2761 atacgaattt gaaatcgacg aaggaggtta gttattaata atctttccag aaaacaattt
     2821 cagcttcttt aatgcgatag ccatccggta aattcttcat ctgaaccgaa tcgagatttg
     2881 cagtaatcgt aagcacatgc ctgcccattc caagttgggt tatcggaata aaagccgtca
     2941 ttccaaaccg gtttagatcc ctactcctgg tcggaatcca ttcgatattg ttatacacac
     3001 tgtcgtcgat acggaccaag gcaaggtcgg aaaaatgttt ttccccgtct ttccctgaaa
     3061 tataatccat aagtttatcc caatctctat cgtaaaccgc aaaaactttc aggcatttat
     3121 ctttaacaat aagagactga atggctatat ctttcacctt tgtaccatcc tgatcatcta
     3181 aataataatt gttgtcgata tatatattgg agcccatatt ttcttgaaaa cggtaggttc
     3241 gctgatcgag aatattcggc caatgcatgg tgcgatacag cgacacgtat gagaaagata
     3301 tcgcggcaag catatacgca aacagaatca gggcaatttt catttttctg atattcgtgc
     3361 ttaacaaaag caacgaacgc tgatatagaa acctcagagt caggaaatca attaatcgga
     3421 atggcagata tacaatgtaa ctcaaataag gtatttttct cagaagccca aacgtaataa
     3481 aatcgaacag gtatacgacg aaaacaacat aaaaaaccgt aaagtacaca gctttgtcga
     3541 ctggtaatgt aataacagga atggtaaaag aggtaataaa aaacaatata aaaccggcta
     3601 aaatcacagc aagaattatc gaaaaataaa taattaaacc gcaaaatctg tcaaccgaca
     3661 ttatctgctt atacattcct gtttctcctt cagccggaat tctgaaaggt ttcctccaac
     3721 caatcttttc ctgtcggaga cctcccggga aaacataact cacacaaacc aatgccagcc
     3781 agtacgacct cattgccaaa tggacaacaa aacccaacgt aaggcttttt aacgcaatca
     3841 taacaaagat catgaaaatc gataatccag gcactgaagc atatttgctg attgaaacaa
     3901 aaaaaccggg tataatgcct cccactttga aaagtgaaaa aattgctcct ccagaaatga
     3961 gtaactcgag ctcccatgag ctttcctgaa gttttttaat ccattttggc actttttgtt
     4021 cttcttgcat ctatttcaac ttttttataa tgaataaaac ctgatcaata tattaccctg
     4081 tttgttgtta cagcttgata aacttcttaa taaagacctt ctctccacag gagaatttca
     4141 acgtgtattg tccgggttgc agatcggtaa tttcaatagt attatcggtt atagtatgaa
     4201 tcgacttttc gcaaaccttt gaacccgaaa tattaaaaat ttctgcttgt gctgcttgat
     4261 ccggttgccc cggaatatac gcattaatta catccccggc agggttcgga ctaattacaa
     4321 taacagcact gttattgtaa gattctgaag gaattgccga gaatagctgg tagttattca
     4381 tatcggtcaa accccgcgaa tcggttactt taatacataa attcgaattc agctcttcgg
     4441 tggtaactgt tcccgataac aatccgctct cggtaagctg aagttcctgc gggatcatag
     4501 aggcatgaaa actatatgcg gttaccggta catctcccgg tagtccgctt aaaggcgata
     4561 aagtgtaatt ttcgagattt ccgttcgaaa cacccgaaat ccaaaccgtg ccattcaatc
     4621 ctttacaatc gttataatat actgtaattg tgccattggc ttcaagcttg agagtggtgt
     4681 ttatatcggt acccgtccag ttaatcagac aataatcggt accctctgaa taaaaagtgc
     4741 tgcaattaac cgacgacata aatgcagcaa tacatttacg gtgcataaag ctaacggtaa
     4801 gtttatttgt agagttgtcg ttgtaaggta aatcataagt atcgttcgaa aagccgatat
     4861 aaccattcga gctcagatag aaaccttgat aggttgagcc ataaaaatcg aactcgaaag
     4921 gcaattcaat ataggtatct attattggag ttgttgcatt gggtgcagtt tcaacgattt
     4981 ctgtttcgtg ataatcgata ttaaattccc agcggtatgg tggtgtgcca ccggcagatt
     5041 ctaactgctg cgaataattt tgattaagaa ccggttcggg caaaacatcc ggagcgattt
     5101 gcggtttttc gaaaacaggt gtatgcacta atgacacagt ggtaagattg ttgttagtga
     5161 tgctgacatc cgattgtgat gaaacaacct caatcggttc ccccgcactg taatccatca
     5221 ccgagaagct gataatacgc ccattacccc atccccagtt gtcgttttcg ataaccataa
     5281 agaagaattt taccggggtt cccgaattaa tcagattcaa catcggagtc acatccagcc
     5341 cgaattccat atatttatat tcatcgccaa tactaccttg cataggcaat tctcctccct
     5401 gataatcgaa aatcggaaag ctcaagtaat aatccggact tgtggcatca aggtcggtgc
     5461 tgaaacctgt cagtattttt attctacccc tgttggaata atacaggttt accttatagg
     5521 taagcaccgg agaatattca ctcatggcat acatcacatt taccgtatga ttccagacac
     5581 cgccattgtc cgattccatt gccaaagtgc ggtacatcat ccatgcgtaa taaggtacgt
     5641 cgtaattatt caccatttta aaagcaccaa tttcccagtc gcacacatcc acaatatcgt
     5701 caccagtgat gtcgatatcg ttggtgtact gcccatcgcc attataatcg tatcgaacag
     5761 agtcgttata gcctataata ctcatggcat gatccggcga atccccccag ctgataatga
     5821 cattttcgaa agcatgcggc gattcgtcag gcaagtaatg cgttgcgccc tgatattgcg
     5881 aatagaaaaa accgataccc cctgtttcgg aaccgtcgag atgatcgtta agccagtttt
     5941 taaccgtcaa aatgccgtct tcggtgtcgc atttaattgc agcagctcca ctgatgcggt
     6001 tgtgcattcc ggcataatag ttaccgtatc ctgtcataaa gcgtgaactt cctccactat
     6061 cgtaggctcc gccataggta gagacattgg gaattccaac ggttttcagt aatttaagcg
     6121 tgtgatagta acttgcccca taccagccat aaccaccgtt ttcccagttc catgcaaaat
     6181 atgtgggata tttgttttcg gtaagtgtgg catcaagccc ccgcaggcgg tcgatttcgt
     6241 aggtaaatgc attggctacc gaagcagcct gaccgcattc atagcctgat tgctcaaaca
     6301 gaccacaatg atatggtagt gtgctgttgt cgacttccac tggcagcgat ttgtttttgt
     6361 acgactccgg caatttcagt tctgggagtg tcgacaattc tgattgttct cttgccgaga
     6421 gcggtcggct cataggctgt tcatcaatag taaaattttg cgccaccaat tgagaaccaa
     6481 tcagtaaaaa cagagaaaca ataaaaacct tcatatatat aattttataa gcagttgaaa
     6541 ttttgcatta ttaataattc gtaaatttta aaaataattt cgttacacga aatataagac
     6601 ctcaatatat tttgaaaagt tgcaaacgaa ataaaattaa accgaagtaa taattcgttt
     6661 agtaatgcaa cctttttata tcttgcatag ttattttaac agatagaata aactaaatgt
     6721 aaattttagc aaactcatac tgtttgttag tcggcctgtt caataaacat atatgattat
     6781 gaaaagacta acacaaattt tagcaattgc ctttgtttcg attgccacac catgcactgc
     6841 ctttgcccaa aacaatttta tcgtttgcgg aatacccgac ttggacagag acacgtataa
     6901 cgatataaac gacactacaa tacaaccata tcatgtaaaa agtgggtcat ggtacaacag
     6961 gtataatttt gatatggatg gcaatggagg gttggatgta aaaatacgga ccaccgaatg
     7021 ggaaaatgca ggaggaggtg gcaataatac tcaattgctt tatctcacta ataatataaa
     7081 atttgctttt ggagagcaac ttgtagacac aatatattac ttcaacccac ctcagattca
     7141 atactacaat ttcccaaaaa cttttaatta caacgatact attgattcac gttataattt
     7201 tttgcaatct gatatgttct tagctgtaat aggcggacct ggtttttatt ttatttatca
     7261 cccgggctgg cttaaccttg gcgaaaaata catcggcatc agtatggaga agaacgatgt
     7321 tatgttgtat ggctatattt tagtggaagt tacagggtac tcccgtgtca taattaaaga
     7381 gtttgccatg aacataaacc cctatgtggg tattgagccc ggtaaaacaa atcaggtttt
     7441 tgagatgtac cccaatcctg ccagtaacga ggtttttatc aaacagtcca accaaaacac
     7501 aggtaatcct gccgtttttt cgctgtatga tataagtggg aaaatggtgt ttcaggcaac
     7561 ttcggttccc gaagacggac acattgcgct gcctgcattg gcaaagggct tgtatgttgc
     7621 cgaatttgag gtggatggac agaggtattc gaaaaaactg gcgattgagt aaatgggcat
     7681 tattgcttat tccccgccaa attccatcag ataggctttg ataaaatcgt tcaaccggcc
     7741 gtccatcaca gcctgcacat ttccggtttc gtgtccggtg cgcaggtctt ttaccatttt
     7801 atagggatgc atcacatagc tgcgtatttg tgaaccccat tcaattttct ttttctgccc
     7861 ttcgattttt tgcttttctt cgagttgttt gcggagttca agttcgtaga ggcgcgactt
     7921 gagcaggcgc atggcgtttt cgcggttttt tagctgcgaa cgggtttcgg tgttttcgat
     7981 aacaatgccg gagggcaaat ggcgtaagcg cactcctgtt tccactttat ttacattttg
     8041 tccgccggct ccactgcttc ggtatgtatc ccattcaata tcggcctgat tgatctcaat
     8101 ttcgatgttc tcgtccacca tcgggtacac aaacaccgaa gcaaatgatg tatgacgttt
     8161 atgcgccgca tcgaaaggag aaagccgcac aaggcgatgc acaccgtttt cgcttttcag
     8221 gtatccataa gcaaacttac ccgcaaactc aaggcttacc gacttcactc ccacagtgtc
     8281 gccgggttga aaatcgattt cgcgcacctt atatccgttt tcctcgcccc agcggatata
     8341 catacgcatc agcatatcgg cccagtcgtt gctttcggta ccgcctgccc ctgcgttgat
     8401 gttcatcaca caatccatag catcttcttc gctacgaagc atgtttttca gttcgagatt
     8461 ttctactgca gtcagcgctt gattgtaggc atgcagaacg tcgtcttcac cggcatcgcc
     8521 tgtttcggca aaatcgatca ttacctgaaa atcgtcgacc agatttttca cgtcgttata
     8581 tgcatcaatc cactgcttaa ttgaactgat gtttttcatg tgtaattcgg ctgccttagg
     8641 atcgtcccaa aaacccggag cttgtgtttt ctcagtctct tcgtttacgc gggctatttt
     8701 gttgtcgagg tcaaagaaac ctcctcaggg ctgcaagccg cccgttaata tctttctgtt
     8761 gttcggtgtt aaccattatc tttttcttgc aaaaatagga tttatatttg aaaggtaaaa
     8821 tcgatcagca acacggcaac atatcggcag aatgtgtatt tttacaaaaa gttaaccaat
     8881 gaatctattt accccggtac ctgttccgcc tcccggtttc aatatcgatt acagtgatac
     8941 gatattttcg atagggagct gctttgccga aaatatttct ttaaaactgg cggaagcagg
     9001 tttcagaatt tgttcaaacc cttccggggt gctcttcaat ccggtttcga cggggaattg
     9061 cattctcgat ctaattgata aaaaaaagtt ttctgcagaa gcgctggagt acgatggagc
     9121 attatatttt cataacggtt ttcatggttc gttttcgtcg ccagataaag agaaatgtct
     9181 cgaaaagata catgaaagca tgatgcaagc ctcagaaaat tttacaaaag caagtattct
     9241 cattgtaact tttggcacat catgggttta tatgcaccga aaatcgggag caattgtggc
     9301 aaattgccac aaacaaccgg cagaaacatt cctgcgcaaa aggcttgagc ccggagagat
     9361 attttcattc tggcaggaga tacttcacag tctgaacgca cttaatccaa atttgaaaat
     9421 aattttcagt atcagtccgg tgcgtcattt caaagatgga gctttcgaaa accagctgag
     9481 caaagccggg ctgtttattg ctgtaaacca actgctggag tcgaacaaaa actgttttta
     9541 ttttccggct tacgaagtca tgatggacga gctccgcgat tacagatttt attcttccga
     9601 catgttgcat ccttccgaaa cggccattga atatatctgg gaaaaattcg gggaagccta
     9661 tttttcggaa aacacacata agctgacaca aaaagcaaca gctatctccc gttcactgaa
     9721 acaccggcca catcattcca actcaactgc ctatgcggaa tttgttaaaa agcttaaaca
     9781 agaagtttat gttttcgaaa aagaaaatcc gggagctgat ttcagtttta gtaaaaaatg
     9841 gctgagtggc aaaaattcac ctgaatcaga ttaattttct gtagtaacag ccactgcatt
     9901 ataattctgg tcgcgctgca ccggaataaa cccgttttgt cttatcaact gcttcatttg
     9961 agctgctata agcgtgtttt cgagtccggt cgattttacc acattttcct ctatcatcac
    10021 cgagcccagg tcgtcggcgc cggcatgcag gcacaaagct cccacttcgg tacccactgt
    10081 aagccacgat gcctgaatat gttgaatgtt ccgcagaaaa atacgactca gggaaatgaa
    10141 cctgacgtac tccgacgctg aaatgggttt caaatcaggg taatgcttca gtaatcttgt
    10201 attgttgaaa gaacatggcc agggtataaa tgcaataaaa cccatgcttt gtggaggttt
    10261 ttcgttttgc aattccctga tgaggtacaa atgttgtatg cgttcttcga ttgtttcaat
    10321 atgcccgaac atcatggttg ccgtagtagt aataccaatt ttatgagcct gtcgcatcac
    10381 ttcgagccat tcgtcaacac tacattttgc agggcttaat tgtttgcgga ccctgtcgga
    10441 caaaatttca gcacctgctc ccgggaggct gtcgagacct gcttctatca gtatttttag
    10501 ggtatcagca attccaattc cttctttttt cgacagaaac acaatctcgg gtggacctaa
    10561 agcatgcagt ttcaggttcg ggtatttatc ctttagcgat gcaaacagat gggtgtaaaa
    10621 acgcaggcca agttccgggt tcaggccacc ttgcaacagc aactggttgc ctccttccct
    10681 gaaaagtgtt tctattcttt catgatagtc attcaaagtt aaagtgtacg cattcgaaga
    10741 tccttttttt gtgcagaaat tacaaaaaag gcactgaacc tggcaaatat tcgtaatatt
    10801 cacattcctg tcgacaatcc atgtaacatt gccaggggtg ttcattgaat tacgcagcat
    10861 gtttgcagag ttcatcagcg caggaagcgg agcatgatag taaaggttta gcgcttcatc
    10921 aacactcaga aaatcggttt cagcagaccg ggcaagaagc tcttttacat aagtggtggt
    10981 catgcatccg agctaaatat ttccggtttt tcgcagttta ttctcatcga gaattttaat
    11041 acgtcttcct tttaattcaa tcatgttctc agatttgaat tccgataaca acctgataac
    11101 agactcggtt gcggttccga tgagatttgc cagttcttcg cgggtaagag taattttaag
    11161 aatattttcg gtatcggtac caaattcatt tttaataagc attagcattt cggccaaacg
    11221 ttcgcgcaca tttttctgag ccatgtcggt aataaagtta ttggcttcac ccaattcggc
    11281 acaggccagt tgaagcaaat cgatggcaaa atcgttgttt ttgcgaagca gggcatataa
    11341 gtcgcttgca attatatggc aaaggacggc atcatcgtgc acttttacac tggtacaagc
    11401 aggttctcca ttcataatgg accgataccc gaaaatatca ccgggttttg caaattttat
    11461 tatctgttcc tttccggcaa caccagtttt gtaaattttt attacgcctg aatgaataca
    11521 gtaaattcca ctgatgcgct gcccctcctt ataaatagca gtaccctttt tgtaagctga
    11581 gcaggttttc cgctcactga cataatcgag ctcttccggt ttcaactttc taaaaatgcc
    11641 tctgttggtt ataaggcaat ctttacaaag cggctcttca aaatgtgaag tcatgatggc
    11701 aatttcaatt ttttacaaat ataaatataa aatgaaagat aaaataaccc aatactcatg
    11761 caattgttta ttgcagtaat ttatgcaaaa gcttataaaa caaataagtg aaaaaagcca
    11821 gaaactaaaa tatgattttg caaatatcaa ttattttata aatttgccat ctaaacagag
    11881 cagcattaga tgagcgacaa ggaaaatcag ttcagttttg acacccgtaa tctttttaat
    11941 ttcattttag cgaagaaaaa accgttaact gttattgtcg gatctgcttt aatagtgtca
    12001 ctgatactta gttttctggt tcccgtaaaa tataaatcat ctgtaatttt atatcccgcc
    12061 tctcccgggt ctgtttcaca aagtttactg tctgaaagtt ttgctgaaaa agaactcctt
    12121 aaattcggag aacaggaaga agttgagcaa atgatgcaaa tattgcaatc atcggctatc
    12181 agagacagaa taatacagaa atatgattta ttcaaacatt acaacattga taaagcaggg
    12241 aaatacccca atacaagcct ttacaggcaa tatcagagaa atgttaaagt tgttcgcacc
    12301 gagtatatgt cggtaagagt tgatgtttac gacgaaaacc ctaaaatggc tgccgcaata
    12361 gccaacgata ttgctgctct ttacgacagc acggtaaaca ttatgcagaa agaacgaaca
    12421 ttaaaagcat ttctacttgt agaacaagag tataatttgg tcaaggaaaa aatccaacgc
    12481 attcaggata ctatcagaac gctgcacgaa aagggtgttt ttgattttga atcgcaaagc
    12541 gaagtcttca acgatcagct tgccttagct ctggcgcagg gaaaccaacg aggggttcag
    12601 gaaattgaaa agcgtcttga tattctcgct gaatatggaa gtcaatacac caatctgaga
    12661 gaccaacttc aggaagaaat taaaaatctt gccaaaattg agtcgaaata cgctgaagcc
    12721 aaggttgatc tccatcagga ccttccgcat aagtatgttg taagcaaagg tgaagttcct
    12781 gagaaaaagg cgtatcctat ccgctgggca attgtagtgg taagcacatt tgcagctttc
    12841 tttttcgcct taatcatact gctttttttg gacccgatta aaaaaaaaaa gaatacctga
    12901 accaattaac ccgattactg aatcatgttg aatttgaaat aaactataat cttatggaac
    12961 aatacttccg aacaaaaaag ctttttgaaa tatcgctgaa gtacaaatgg catttgttaa
    13021 ctattgccgt tgttgctgcg gctgttggtg tgattgtttc ttcgccgtgg gttataaaac
    13081 caaaatacaa atcttttgct gtggtttacc ccgccaatgt ctccccattt tcggaggaat
    13141 cggaaacaga gcaattgctt gaggtaattc agtctcttga tatccgtttt cgcgtttgtg
    13201 aaacattcca actgggagag cattacggta tcagtaaaaa caacccacat tttcagactt
    13261 tgctgaacaa agaatttgaa aacaatgtaa cttttcagaa aactccaaac gaagccgttc
    13321 aaattacggc attagacacc gacgcacaac ttgcctccga tattgttgat tcaatacttt
    13381 cgttttacag ccaaaagatg cttactatga tccggtcaaa gtcgaaagaa attgttctca
    13441 tcaagcaggg tcaaatgaac agaaaagaaa aagaaataga cagcctgact aatattatca
    13501 acgagtatcg gcaaaaaaac aacttacttg attataaaac tcaggtgaaa gtttattccg
    13561 aagctgttag cggtgggaaa agccttgaag aagcacgcaa aatgctcgat aactggaaac
    13621 agcttggttc tgagtatatg aaaaccgact caaccctttg gtttgcaatc accgattttc
    13681 acagattaaa aaaagattac gacgaagctt ttatgcactc tattaaagag ctgaccttta
    13741 ctcatattat aacgcatcct tttccggcgg ataaaaaatc atatcccatt cgttggttat
    13801 ttgttctttt ttcggtaata ggttctttat ttgcttcatt cctgataatt gccatcatcg
    13861 aagggcgaaa ggaaaaaacc aaaaacggtg ctgtctaagc gaaagataat tgcattttat
    13921 gtgctttcaa tactttttat tgtattgaat gttttgtttg tatattttac ggaatctctt
    13981 gttttcaacg cacttccttt tatcctgctt ttctttctgt ttgcattgtt ttcgctggat
    14041 aaaatgctga ttgtttcgtt tgctttggtt ccactttcga tacccctgaa agaattgctt
    14101 ccgggattgg attacgacat gtcgcttcct acagaacctt tgttatttct aattcttctt
    14161 attttcattc taaaacaact tcgcgacaga gatttcgata ccgctgtgag caaacatata
    14221 ataagtaaag ttctgtattt ttacctgggc tggatggcaa tcacagtgat tacaagtaca
    14281 atgcccattg tttctttgaa atttttgctg gtaaaacttt ggtttatcat tcctttttac
    14341 tttcttctta cacaagtatt taaagattat aaaaacatca ataaatcgtt ttggttttat
    14401 atcattcctt tcattattgt aattgcttac accttattca gacattccgc attctatttc
    14461 gatcagaaat cggcgaatta tgtcatgaca ccttttttca acgaccacac gtcatatggg
    14521 gcattgctag cgatgtacat tcccattttg gttggaatga taaccgataa atcgcagaaa
    14581 aaagctgtgc ggctaattag tgtttctgtg cttgccattt ttgtgcttgc tattattttt
    14641 agttatacac gtgcttcatg ggtaggttta attgccgctt tgggactatg gtttttacta
    14701 aaaatgcgca taaaaatcag gactttgttc attggggttg gtatcgttct cacgttattt
    14761 gttttgtatc agtcgcaaat catgatgagc cttgaacaga acaaacagga ctcatcaaca
    14821 aatctcgaag aacatgttaa atccatttct aatattgcta ccgatgcaag taatctggag
    14881 aggataaacc gatggaattg tgcggtgaga atgttcaaag aaaaaccatt atttggctgg
    14941 ggtccaggga cataccagtt taaatatgca ccatttcaat tctcttacga aaagacaatt
    15001 atcagtacaa atgccgggga tatggggaat gctcatagtg agtatctcgg acctttggcg
    15061 gaatcgggtg tttttggcct gttgtcgatg attgctattg tgatagtgtt ttatatcaca
    15121 tctataaacc tgtactataa acttaccgat ttcaagacaa aaacactggt tatcagcatt
    15181 atatgcgggc ttacaacata ttttattcat ggcttgatga ataatttcct cgataccgac
    15241 aaagcatcag ctccattttg ggcatttttt gcaataattg ttgccattga tgtatatcat
    15301 aaacccaaaa gcgacaccga aattcagaca gaaaaataat tagtttgttt gaacaaaaag
    15361 gcctgatttt tatttatctt tcgtgtcatt tttaatttag caaaattatg cctaaagcaa
    15421 taatttcgtt tttgatttta tggtctatta gcttacctct ttattcacag ataaacagct
    15481 acccatacaa cgaagggttt gacgttgcat ccattccttc cgaatggact caggaatttg
    15541 tatcaggagc tgttctatgg tcgattggca gtggtggtta tggtggatat ccttcagctc
    15601 cctattccgg aagtggaaat gctcttttca gcagtcctaa ctacaatagc gacgaaacaa
    15661 tgctgataag ccctgaattc gatttatcag ctgtgcaaaa tccaaggctt cggttttatc
    15721 acgcgcaata taactgggat accgatcaag atgtaatgag tgtttattat cgacaaggag
    15781 aatcgggaga atggattttg atttcgagct ataacaccgc tgtaaccgca tggacgcttc
    15841 gggtaattga attacctgaa ccatcttcgt catactttat cggatttaaa gcaacctcgg
    15901 gatatggttt tggtgttgta ctcgatgatg tagttgtaga tgatgccccc caatgcaata
    15961 ctccctcaga atttagtgtg ttgagtacct cgaccgaatc ggcaaacttt aactggacgt
    16021 cggaagccaa cctttggcaa cttgaatacg gacccgtagg attttcgcat ggtggaggaa
    16081 tcttagtcga aaatattaca agtgcgccgt tcaccttgtt tgggttaacc gaggggcaat
    16141 cgtatcaggc atatgttcgg gctgtttgct ccggaaataa cagtgaatgg gcaggtccgg
    16201 ttagcttcac tgccgattgc aatttaataa atgcaaataa ttttattgaa gggtttgaaa
    16261 gttcatctgc tcctccagct tgttggtcgg tagtgtatca gaacacaacc ccaccggcag
    16321 gaaacctcgt aacacataca acaacctatg ccttctctgg caataggtcg tttcgtttca
    16381 gttcctattc tccgggacct ccctacgggc aatacctcat tactccggaa ataggctctt
    16441 acacggggac aagagaattg cgattttggt ataaaagata ctcttcagga agcgaaactt
    16501 ttcgggttgg gtatagcaca accggaacca accttactac cgactttaac tggaccgaag
    16561 aaattactga tgcaacagca caatggaagc tttacaacat ggtattgcca ccaaatgtta
    16621 agtttgttgc aatcaactac cggacagttt ttcagtatta tttatatctg gatcagtttc
    16681 agatacgcat tcccccaacc tgcccccagc ccgagaatct acatgtcagc aatattactg
    16741 cagtttcagc tcatatcaac tggcttgcag gatccaacga agaatcatgg caggttgaat
    16801 atggattttc tgggttttcg catggcgaag gcactattac caatacatct tttcccgtgc
    16861 atattcttca aaacctgagt ccgaattcaa actacgatgt ttatgtgagg gctctttgtg
    16921 atgaagaaaa cagcacatgg gttggtcctt tatcgttttc cacaccatac gggtgtcagc
    16981 aaataaccaa tataagcata agtaatgtca gtgccacatc ggcttttatc gattgggtac
    17041 catccggttc agaagaaagc tggaatatgg aatacggaat atccggcttt accccaggaa
    17101 ccggcaccat tatttccggc ataaccgaaa gccaatattc actaaccgat ttactgccaa
    17161 atacaactta tgatgtaaga atccaggcat attgtggaaa cgtatacgga gtatcgctgt
    17221 ggtcgaattt ttcaacattt ctcacactcc cttgcaatga tgggtgcaat tacactttgc
    17281 agcaatcgga tgcatggggc gacggctggg gcgaaactta tatttcggta ttgcaaaatg
    17341 ggattgaaac taataaagtg tatatgacca gcggctcatt ccaaagtgca gatatatact
    17401 tatgcaacga agcctcggta agtctcgtta tacatccttc atctttcgtc agcgaggttg
    17461 gattaaacct gcttgacgcg tttggtaaca gcatttatca attacctatc agcaccctcg
    17521 aagaaggaat ctcggttaca acactggtta cattttttgc atcctgcaca gaacccgaat
    17581 gctatcctcc gataaatcca gcaattacca gtattcatgc tgaaggtgca gtggtttcgt
    17641 gggaagcatc ggcaagtatc gggaattatc aggtaagcta cgggtttagc ggttttaata
    17701 caaatgaggg ctatcttagc gaggacctga cagaaaccaa ttatactatc tctggcttgt
    17761 ttccccaaac cacctatgaa gtttacatcc gtaccttgtg tacttccggt aacagcattt
    17821 ggtcgggacc ctattctttt acgactactt ccatgaacct ttcaaaccct attgcttgta
    17881 acgcagccct gaatattccc gacaatagct gtgttagtta cagcatttcg gtaaatgacc
    17941 ctgaaaacga aacactaggt atggatgttc ttattgatga gatccgtctt attatgaccc
    18001 ataactttga cggtgacatc gatttaagcc taagctcacc tgatggcaca acggttcttc
    18061 tttttagtga tgttggagga agtggcgata actttggcaa taacaacgga agttgcaacc
    18121 aatacacaag cctcgccatg gatggcgcta gtggtgcaat tacttcaggc acagctcctt
    18181 ttatcggttc ttttattcct gaaggaaata tcaatgcatt ccataatggc ggaaatatta
    18241 atggcgactg gacactgcag atatgcgatg acaatgctgc ttttactgga acacttcagt
    18301 atttcgaaat cgtgtttact gaacaaaaat atcttgaatg gagttctctg caatttgtag
    18361 aaagccccga aaacaatggc agcattagta actcagtgaa ccttggttta tacaacgaaa
    18421 catttgctgt gtcgggccct ttgaatgagg gagcgcacta tcttacttcg aatattcctt
    18481 cagggcttac tgcccatgtc gaagttgtat cgcccaattc ggcattactc acccttacag
    18541 gctatgcttt aaatcattct aatgccagcg atataaacaa tatatctatt gaatttttaa
    18601 atgctgcttt ttcagggggg caggcttccg aaattgttaa ttataaacag ctttcactga
    18661 gtatcgattt taaaaaccta acagacattg ctaacctgac atcatcggaa gatgaatatg
    18721 tttgtgaaac agagctgaac aattattttg ttcaatatat gtttgtaaat accggtgaaa
    18781 acattattcc cgctggaaca acatttcaaa tgagagttga agatgcttcg gggaatatat
    18841 tgttatcaga aaacatcatc ctcagcaaca acctcgacct cggcgaggat ttaggcggca
    18901 tttcggtgaa ttccattcac tttgccgata ttggattaca tagtgtgaaa atcattgtaa
    18961 aagccccttc cgacatttta cctataaacg ataccattgt aaaaaatatc gttggggttg
    19021 aacaaaatat cgtcttctgg tttgcagaaa acgatagcct taacgttact gaataccctg
    19081 ctattgttgc tgcctggatg gattttaatc ctgattcaag ccttaattat acctattact
    19141 gggaaaacgg tatttccaca tctaattcaa ttgatgttat tgctgatgga tggtatcatt
    19201 gtacaataac aagtgaagca tgccctataa ccgac
//
LOCUS       DAOOQP010000049        19046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3522_length_19046_cov_7.263125, whole genome shotgun sequence.
ACCESSION   DAOOQP010000049 DAOOQP010000000
VERSION     DAOOQP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_19046_cov_7.263125"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLK75_06745"
     CDS             complement(<1..308)
                     /locus_tag="PLK75_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOY38982.1"
                     /translation="MPAENKENLNFIEQIVDNDLKSGKHQQIITRFPPEPNGYLHIGH
                     AKSICLNFGLKRDFDGKCNLRFDDTNPTAEDIEYVNSIMEDVRWLGFEWNNLLYASDY
                     "
     gene            399..2444
                     /locus_tag="PLK75_06750"
     CDS             399..2444
                     /locus_tag="PLK75_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY38983.1"
                     /translation="MKHLIFYVFIAFLFVTGIGDFVFAQNKKPVPVKGFIDVGCLETE
                     EHNTIKLNGEWEFYNGQFLDADDILNGKFSEASFISVPGKWGNLQSLSKGKPGTGFGT
                     YHLVIVAPKIHLGLSFKKIHSASRIYINNDLVSYNGSPSFSREGEIPLSRLQYYYFDN
                     TSDTLHLIIQVSNFHHMHGGIEGSIIIGKTESIRADEYMTVHTTIFGLNLLFVAAILF
                     LLFFAFRRNDLVHLYFALLLLIVILRITAHNDIIMYEWLKATYPLTIRLDFLSENLLA
                     IGMLAYYFTMYPQQQLRRLNLAIIAALSIIALSAFFVPLYTLSNMIFYSRVYGILLLL
                     YATGIIIYRITHSEKTDFLIIFALSLFIIYNFLVVFEEKNSFEIHDLLILLIIVLFVI
                     SQIALLAKRHAQLLRNQEVLTDEVQQLNLELEERVKIRTVLLEKKNKSLIESKNKLEH
                     YLKTKDKLFSIIGHDLRSPLATINLYGEKLWDEISDENQRKKLKYIINGSASALHLLS
                     NFLAWGKRELGQIQISPNFVAVDQLIQESVEAFHGIAEAKSLTIWVESDNSVVYADRF
                     SMLIVVNNLLNNAIKFSYADSQIRVSVQRIAAAPDGKVKISVADTGVGMNQNTIDKLL
                     KSTEISSSLGTANEKGSGIGLFIVKDFVKMNHGELIIESEPGKGSIFSVILQTKIWS"
     gene            2444..2806
                     /gene="folB"
                     /locus_tag="PLK75_06755"
     CDS             2444..2806
                     /gene="folB"
                     /locus_tag="PLK75_06755"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOY38984.1"
                     /translation="MGRITLEGMEFFACHGHYAEEKESGTRFIANVWMDTNTEIAASS
                     DNIADALNYQEAYVCIRRVFEQEQYNLLETLASHCIDSLFEKFSKLEFAGICVRKINP
                     AMGGVIAGVSICIERSRK"
     gene            2881..2952
                     /locus_tag="PLK75_06760"
     tRNA            2881..2952
                     /locus_tag="PLK75_06760"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2914..2916,aa:Glu,seq:ttc)
     gene            complement(3027..4538)
                     /locus_tag="PLK75_06765"
     CDS             complement(3027..4538)
                     /locus_tag="PLK75_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY38985.1"
                     /translation="MEKKIRILHIDDSMHDRLLVKDALLREDDKFEIIEADNREVFEH
                     YLKNEEFDLVLSDFNILGFDGLQVIQFVKEFQPGVPVIIVTGTGSEEIAIQAMKMGAS
                     DYVIKSVSHIQGLPHAIDTIMELKKLNDERIKAEKALKESEQLFHNLSHISPVGIFRA
                     NTEGNLTYVNPMCLSMTRLNPKDISSGNFMNRIHPEDRKRIVDLWYNSITAQEPFMAE
                     WRFLKPESGVLWVLGNAVPEVNGNAYTGYIGTLTDITDRKSAEEEIILAKEKAEECDR
                     LKTAFLHNISHEIRTPMNAIIGFSELLESHDLTPDKRMEYSTMIIKSSNHLLGIINDI
                     ISISTIEAGQVKTNKQALVVKDLCSLMYNKYSAKAKLQNLTFSYVTSNTPDDFSIVTD
                     ESMLTQIIDNLVNNALKFTRKGSVVLGYEKIGEFVQFFVADTGIGIPKTLHEEIFKRF
                     RQVDTGYTRQYGGSGLGLSISKAYVELLGGSMRLESEMGKGSTFYFTLPASNK"
     gene            complement(4556..4999)
                     /locus_tag="PLK75_06770"
     CDS             complement(4556..4999)
                     /locus_tag="PLK75_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY38986.1"
                     /translation="MENLATILLVEDNQMDVILTLDAFREAKLKNKISVARNGQEALD
                     YLFGRNKFSDRSEFPLPNLILLDLKMPGIDGFEVLRQIKSSDILKRIPVIILTSSKED
                     GDRALSYDIGANSYLLKPVSFDGFTEVVKKIDDYWFTLNIGAPGL"
     gene            complement(5004..6836)
                     /locus_tag="PLK75_06775"
     CDS             complement(5004..6836)
                     /locus_tag="PLK75_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HOY38987.1"
                     /translation="MKNVCHSVFYFIAFTLMSMYVQAQQVSREAAMSAYIYNFAKNVE
                     WQNETVISDFHFLIIGQDQKIINELNNLAKTKTLRGKPITVSSATTFKPGVACQLIFV
                     TNDSEEMLVDIFDKIEGKNILLVSDEYKDENLIMVDFYTSKDGTLLFKINRSNILNQN
                     ITIMPDMVLTGGTEVDVAALYHEGQESLRSLQKRAENLENNLNKLQQEVIERNEEVQM
                     GRDSLRRQSKQIQEQQLVLEHQQSQLCKQTAELQNQKLQIVEQNNTFKLQSKDLENQR
                     ASLEEGAQLLKEQEQELEKQKTHIAAQKKVLKEQGSKIHNQRKILYLLLIIILLVFAV
                     VALIYFAYRNKQKHNRELEMRVEERTRELHLLNLTLEDKVTERTAQLVAINKELESFS
                     YSISHDLRAPLRAIFGFSQILSRRHTNSLNEEGKQYMNYIVEASIRMEQLINDLLNYS
                     RLGRKSIDLHPVSLYKIAEKLKTDFKQRIEEVGGVLRTDSSFPEVKGDESLLMQIFNN
                     LIENAITYRRTDVMLQIDISCTTSDNFATVTVADNGIGIPREYWEKIFNIFQRLHSED
                     QYPGTGIGLATVQKAVAMINGTISVDSVVGEGSRFIIELPLCKN"
     gene            complement(6846..8807)
                     /locus_tag="PLK75_06780"
     CDS             complement(6846..8807)
                     /locus_tag="PLK75_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY38988.1"
                     /translation="MKSPIFVGLILFFLIIAGIISAQEDTVDIYDMDFTELSKLKIVT
                     ASKTEQEIKEVPSTIIIITVEQIKQNGYFTLEEVLADLPGFQFRNILGINSYVFQRGI
                     PNQNNLTLLLIDGIMVNELNSGGFYGGAQYNLSNVERIEIIAGPASVAYGTNAVTGII
                     NIITKAPTENKFEIKSGFGSFNTYESNVNFMFYNPEKNFGITVSGMLKNSDKADLKGA
                     AGDNNWTDLMDNFERDYAADLKIQFKDFTFGTGFINKRASTATYNKSVGTIYRDYGTE
                     WNICFLNNYLKFSRNLTPKLHLSSLLYNRNATVMPNSVYSVLDTAQVGYYRPNNLTGF
                     ENILHYTACDFFAVTGGLTFEYEFLSEKASYSYSDSAVLKPPRPDRPNLLNNYLASVF
                     VEPRLTLFKNIYISGGVRFDYSSIYNQVLTPRAGVSYVLGRHLFRFSYAQAFRAPKPW
                     DYTDGLGNADLMPEKMQSLELSFVFNITRKVNFTVTGYNNKLENAITKQYIGDAYRWY
                     NIGEINIRGAEASFGYSSRKIRASLNYTFTNSCSYSDPIAEISPHCTNAMISYFLNEY
                     FSLNLRAHYVGQRENPKTIQSTGSNFVDAFIVFDGAFSLLNYKNFTSQLVVKNILNQE
                     YYHTSNRDPDRYRQPQRTILLSVAYVLHY"
     gene            complement(9143..10129)
                     /locus_tag="PLK75_06785"
     CDS             complement(9143..10129)
                     /locus_tag="PLK75_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY38989.1"
                     /translation="MSDSIETYKNDIFNKLFEYSFNTLAMRAFVIQYECCKPYRRYCE
                     LLHKSPDNVRQIRNIPFLPIEFFKNNQIVCGEKHRTSVFKSSGTTGTNSYHYVHDLNL
                     YDRNLDEGFRSFYGAPAQFRFIAILPGYIERNDASLIYMLRQLIAAGGHSESGIYSSG
                     DDEVFLKIQKLEAVAQPYFLWAVSHALMKFAEQYPMPVQHCIVMETGGSKGMGKEIIR
                     SEMHGFLKKSFSVKTIHSEYGMTELLSQAYAVSDGIFRPTPRMKVLVRDATDPLCISE
                     AGTGMLNIIDLANIHSCCFIGTSDVGTVQSDGSFELLGRADNSDARGCNQMV"
     gene            complement(10172..10972)
                     /locus_tag="PLK75_06790"
     CDS             complement(10172..10972)
                     /locus_tag="PLK75_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38990.1"
                     /translation="MKKYLLFISVIITFTGGYNLKAEEYIVKGLYQGIDLYVINPMLD
                     DEGYTYCVTEIFINNNVYEDVLNSSAFCISLSKLDLKVGDGLEIRFVHSNDCAPKIIN
                     PEVLKPITTFTVTAINITGNVLNFSTTGETSKIPFQVEQFRWNRWITVATVEGKGGPG
                     ENTYTAAILPSSGENRYRVIQKDHLYRTRFSDEYLYTNTQEPVVVTSKLKKVKTEITL
                     SAASMYYVVNSFGATVLEGSGEAIDVSSLPKGKYILAYDATSVGFTKK"
     gene            11178..11834
                     /locus_tag="PLK75_06795"
     CDS             11178..11834
                     /locus_tag="PLK75_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38991.1"
                     /translation="MKNIALLITAVILSNFIFSQEFETTTDYYKNGVKKSEGMMHLGR
                     EMGHWQYFDSNGKLVRETDYYKGELYGNDITYYPNGNKNEEFTFESNLKNGSYKNWYE
                     SGVLRTEGFYKYDVKDSSWTTYYNTGKPEMEVLYNQGSGRIISFYSPKNEKPLQMAMV
                     PFCGITSFLPNYLKGLLPMVWSKVFGHIISKMEILKKRALSTMETVSEHGKDIMKTNN
                     "
     gene            11717..12976
                     /locus_tag="PLK75_06800"
     CDS             11717..12976
                     /locus_tag="PLK75_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOY38992.1"
                     /translation="MEQGLWTYYFENGNIKETGTFDNGNRIGTWKGYYENKQLKSDIN
                     YENGKCVYYYENGTKEMEGIMKNKERDGEWVFWYPDGNIRSRNNYKSGVRNGIQEKYY
                     SNGNLEARINYIDGKKNGEAHWGLQDGKKDIDGNFVNDVQEGLWTYWRTDGTKGNEGY
                     YLNGEMSGKWTYWYGNGQVWRIINYEHGRKNGDYIVYYENGNVFRKGQFENDVEIGLW
                     TEYYENSAKSLEGNYDFGVMTGLWNSWYDNGEKKSAIHYKNGLITDKALYYYPNGKIQ
                     IEENYVVYENKGNPDLYGESREVSVLNGVTRTYNLKGTILMEGNYLLGKKTGKWTYYN
                     PQGGKIRLENYKDDKPDGKWQGWYDHGVQEFEMNYKNGLLEGRMVTFDQQGNVEYDAI
                     YRGNYEVEVKVNNNDPQKENQRDDYRR"
     gene            complement(13299..14633)
                     /locus_tag="PLK75_06805"
     CDS             complement(13299..14633)
                     /locus_tag="PLK75_06805"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOY38993.1"
                     /translation="MKTVLILGAGLSATSLIEYLLEGSEKYNWQIIVGDSDPAVASRK
                     IKAHPNARSVFFDVLDNSLVQQYVSQCDTAVSFLPARFHPIIAKACAEHGKNMVTASY
                     VSPEMRSLDAEAKAKGLIFINEVGVDPGLDHMSAMHVIDEIRNKGGKLLSFKSSTGGL
                     IAPDYDDNPWNYKFTWNPRNVVLAGQGTARFIRNGRYKFIPYHNIYRRVENTEIPGYG
                     EFEIYANRDSLSYRETYGLIDIPTIFRGTMRRPGYCEAWNVFVQLGITDDTYIYEGSE
                     NATYRDFINSYLRFDPNAKVEDKLCDFLGLDRNGEIMKKLEWLGIFEERRINMKNATP
                     AQILQKLLEEKWALGADDKDMIVMQHQFEYELHGKRYGIKSSMVFIGKDQANTAMAIT
                     VGIPAAIATKMVLIEAFTKPGVHVPVTPDLYGPILKELETYGIRFSEEHFEL"
     gene            14775..15197
                     /locus_tag="PLK75_06810"
     CDS             14775..15197
                     /locus_tag="PLK75_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOY38994.1"
                     /translation="MKQVAVFLLAVFSALVMQAQDVTGKWITVDDQTGIEKSVVQIYE
                     KDGKLYGKVIAIIDKSKGENPLCTKCSGTLKDKPILGMIVVRGLSKTKDGWFAKNAFL
                     EPESGWIVDGKLWLEGNELKVRADVGIFHRTQTWKRLR"
     gene            complement(15217..16470)
                     /locus_tag="PLK75_06815"
     CDS             complement(15217..16470)
                     /locus_tag="PLK75_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOY38995.1"
                     /translation="MGLKVRLIIMNFLQFYIWGSWLITIANYWFGTKGWSGAEFGIIF
                     STMGIASLFMPALMGIVADKWVNAEKLYGILHLCGAVSLFLIPQINEPTMFFWGILLA
                     MIFYMPTIALTNTIAYTALKSNNYDVVKSFPPIRVFGTIGFIAAMWIVNLTGNKSSEN
                     QFFIASAISLLLGIYAFTLPKCPTFAQTSQKKTFIQMLGLEAFVLFKNKKMALFFVFS
                     MFLGAALQLTNAYGDVFLDDFKNIPQYADSFVVKYSTIIMSISQMSETLFILAIPFFL
                     KRFGIKKVMLISMFAWVLRFGLFGLGNPESGLWMIILSCIVYGMAFDFFNISGSLFVE
                     TQTDSKIRASAQGVFMMMTNGFGAILGSWTSGLVIDKFFTIDGMKQWQGIWISFAIYA
                     FIVAILFALLFKHKHTPEDIGAVQH"
     gene            complement(16522..17112)
                     /locus_tag="PLK75_06820"
     CDS             complement(16522..17112)
                     /locus_tag="PLK75_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOY38996.1"
                     /translation="MKKVRLQVLGLSYSQTQAGAYALILSVENSDQRIPIIIGSFEAQ
                     AIAIQLEEIITARPLTHDLFLHFAQRYEIELKEVIIYKLEEGVFYSQLIFRNKKQLEL
                     IESRTSDAVALALRFKCPIYTTDEIVEKAGIILNLPDDEGPKATEEPQKEQRPQKDIF
                     SLSDGELEQMMNEAVSDENYELASRIRDELNKRKNV"
     gene            17195..18508
                     /locus_tag="PLK75_06825"
     CDS             17195..18508
                     /locus_tag="PLK75_06825"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOY38997.1"
                     /translation="MKLKALFPLIFVFAPLCLFAQHKNSETARHFTDLLEKGNYTEAS
                     KLLQANPPTDSAFWANAWSKFIKNYGHITEERSLESDIFGKYDMVYRIVDTEKKTIKL
                     NYSFENGNLTGFRYQVYDTANAHHAIYANPLFKEKKLRFQSGNFSMSAALLEPAVNKD
                     SIIVIFVHGSGSTDRDESVGGSKVFLDLAQGLASYGIASFRFDKRSYVYPEAFSNRSF
                     TVYDETVQDAVSAVDYCHHVLGYAYSNIFVLGHSLGGMLAPRIANSIATPVGGIIIFS
                     GNARALQTLIGEQTEYLMKRNGKLTKEDKKRLVMMNELNNNVSKLQTGDAAKNTLALP
                     SGLPASYWYDLKNYDQCKTAASLDIPILVMQGGRDYQVTTEDFLLWKKALKGKANASF
                     MLFNKLNHLYVAGESKSYPEEYEKPGNVDKSAIEAISDWLKLQEK"
     gene            complement(18607..>19046)
                     /locus_tag="PLK75_06830"
     CDS             complement(18607..>19046)
                     /locus_tag="PLK75_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY38998.1"
                     /translation="GHWTGTAEAMFGATWNIDITFTETGHYIATASNVSNPNYWPSPF
                     YFGYKMVDNPAGGQMCIEPSADIPCQTFTIDEVINNKGYGVLNVGYEYAMSAYPYSYE
                     CSNNFEIQHLSFSDGGDAVFFSLVEFGTDYYDWYLRYNLIRVD"
BASE COUNT         5722 a         3911 c         3752 g         5661 t
ORIGIN      
        1 aaataatctg aggcatacaa gagattgttc cattcaaaac caagccaacg gacatcttcc
       61 atgatactgt ttacatattc aatatcttcg gcagttggat tggtgtcgtc gaaccgaaga
      121 ttgcatttcc cgtcgaaatc ccgtttcagt ccgaaattca ggcaaatcga cttggcatga
      181 ccaatatgta aatatccgtt aggctccgga gggaaccggg taattatttg ttgatgtttt
      241 cccgatttta aatcattgtc tacaatctgt tcaataaaat tgaggttttc tttgttttct
      301 gcaggcatat aaaagtgcat aagtgtttac gctgtaaaaa taatacttat attttgcaat
      361 aatcaggata aaaatttaat tttatttttt aaaaatatat gaagcacctc attttctatg
      421 tatttattgc tttcttgttt gttacgggaa taggtgactt tgtatttgct caaaacaaga
      481 aacccgttcc ggtaaaaggg ttcattgatg ttggctgtct cgaaaccgaa gaacacaata
      541 caatcaaact gaacggagaa tgggaatttt acaacggaca atttctggat gcggatgaca
      601 ttcttaatgg caaattttcg gaagcttcat ttataagtgt tcctggcaaa tggggaaatc
      661 tgcaaagctt gtcgaaaggg aaacccggaa cagggtttgg cacctatcac cttgttatcg
      721 ttgcacctaa aattcatttg gggttaagtt ttaaaaaaat acatagtgct tcccgtattt
      781 atatcaacaa tgaccttgtc tcgtacaacg gaagtccctc gttttcaaga gaaggagaaa
      841 taccactatc gcgattgcaa tattattatt tcgacaacac ttccgacaca ttgcatctga
      901 tcattcaggt atcaaatttt catcacatgc atggaggtat cgagggatcg attataatcg
      961 gaaaaaccga gagtatccgg gcagacgaat acatgaccgt ccacacaact attttcgggc
     1021 ttaatctatt gtttgtggcc gccattttat tcctgttatt ttttgcattt cgccgtaacg
     1081 accttgttca cctgtatttc gcactattat tgctcatcgt tattctccgc ataacggctc
     1141 ataacgatat catcatgtat gaatggctta aagctactta tccattaaca atccgcctcg
     1201 attttttatc tgaaaacttg ctggctattg gcatgttggc atattatttc accatgtacc
     1261 ctcagcaaca actccgaagg ttgaatttag caattatcgc agctctttcg ataattgctc
     1321 tttcggcttt ctttgttcca ctctatacat tgtcgaatat gatattctat tcacgggtgt
     1381 atggaatact tcttttacta tatgctaccg gaattatcat ttaccgtatt acacatagcg
     1441 aaaaaaccga ttttttaatc atttttgctt tatcgttatt tataatttac aattttctgg
     1501 tggttttcga agaaaaaaat tccttcgaaa ttcacgacct gcttatttta cttattattg
     1561 tactgtttgt tatttcacag attgcccttt tagcaaagcg ccacgcgcaa cttctccgaa
     1621 accaggaggt gctcaccgat gaagtgcagc aacttaatct ggaattggaa gaaagagtta
     1681 aaataagaac cgttttgttg gaaaaaaaga ataagtcgct tatcgaaagc aagaacaaac
     1741 tagagcatta ccttaaaacc aaagacaaac tcttcagtat tattggtcat gatctcagaa
     1801 gcccgcttgc aacaataaac ctttatggag aaaaactttg ggacgaaata tcggatgaaa
     1861 accagcgtaa aaagcttaaa tacataatca acggttcggc ttcggctttg cacctgttat
     1921 caaatttcct tgcatggggg aaaagagaac tgggacaaat acagatttcg cccaattttg
     1981 ttgcagttga tcagcttatt caagaaagtg ttgaggcttt tcatggcatt gctgaagcaa
     2041 aatcactaac tatttgggtt gaatcggaca attctgttgt ttatgccgat cgcttcagta
     2101 tgctgattgt agtaaacaat ctgcttaata atgctattaa attttcgtat gctgattccc
     2161 aaatacgtgt tagcgttcaa cgtattgcag ctgctcccga tgggaaagta aaaatatcgg
     2221 ttgccgacac aggtgttgga atgaatcaga atacaataga caaactgctc aaaagcaccg
     2281 aaatcagttc aagcctgggt actgctaacg aaaaaggttc cggaatcggt ttgtttattg
     2341 tgaaagattt cgtaaaaatg aaccatggag agttgattat cgagtcggaa ccaggtaagg
     2401 gaagcatatt tagtgtaata ttgcaaacaa aaatctggag ttaatgggac gaattactct
     2461 tgaaggcatg gagttttttg catgccacgg gcattatgcc gaagaaaaag aaagcgggac
     2521 ccgctttatc gcgaatgtct ggatggacac aaataccgaa attgcagctt cttccgataa
     2581 tatcgccgat gccctgaatt atcaggaagc ctatgtatgt atccggcgtg tttttgagca
     2641 agagcaatat aatttgcttg aaacactggc ttcgcattgt atagattctc tttttgagaa
     2701 attcagtaag cttgaatttg ccggaatatg cgtccgaaaa ataaatccgg ctatgggtgg
     2761 agttattgca ggcgttagta tttgtattga gcgcagcaga aaataaaata attttttaac
     2821 ctattgcaaa attaaaaaac ccgacttatt tttgcagtcc ctaattagac atgggtacac
     2881 ggtccggtcg tctaggggtt aggacgtcag attttcattc tggtaaccgg ggttcgattc
     2941 cccgtcggac tgcaaaaaag agtcggattg aagaaatccg gctcttttct ttaaaacaat
     3001 aatcattttg ccgattttgt tttggatcat ttgttactcg caggcaaggt aaaataaaaa
     3061 gtacttcctt ttcccatttc cgattcgagc cgcattgaac caccaagtaa ttcgacgtaa
     3121 gctttcgaaa ttgacaaacc caaaccagag cctccatatt gccttgtata gcctgtgtcg
     3181 acctgacgaa accgcttgaa tatttcttcg tgcaatgttt ttggaattcc tattcctgtg
     3241 tctgctacaa aaaactgaac gaattcacca atcttttcat accccaaaac cacggaccct
     3301 tttctggtaa atttcaaagc attattaaca aggttatcaa taatctgtgt aagcatcgat
     3361 tcatcggtta caattgaaaa atcatcagga gtatttgagg taacatacga aaaagtgagg
     3421 ttttgaagtt ttgcctttgc agaatattta ttatacatca gggaacacaa atctttcaca
     3481 accaaagctt gtttatttgt ctttacctgt ccggcttcaa ttgttgaaat gctgataata
     3541 tcgttaataa ttccaagtaa atggttactg ctttttataa tcatagtgct gtattccatc
     3601 cgtttgtctg gtgtcaaatc atgggattca aggagttccg aaaacccaat aattgcattc
     3661 atgggagtac gaatttcgtg cgaaatatta tgcaagaatg ctgtttttag ccgatcgcac
     3721 tcctctgctt tttcttttgc caaaataatt tcctcttctg cactttttct gtcagtgata
     3781 tcggtaagtg ttccaatata gcctgtgtat gcatttccat taacctcagg gaccgcattt
     3841 cccaaaaccc ataaaacgcc gctctcgggt ttcagaaagc gccattctgc cataaacggc
     3901 tcctgtgcag ttatagaatt ataccataaa tcaactattc gttttctgtc ttcgggatga
     3961 attctattca taaaatttcc actcgaaatg tccttggggt ttaatctggt catgctaagg
     4021 cacatagggt taacataggt taaattgcct tcagtatttg cgcggaatat tcccacagga
     4081 gaaatgtgtg atagattgtg aaataattgt tcgctttctt ttagggcttt ttcggctttt
     4141 atgcgttcat cattaagctt ttttaattcc attatggtgt cgatggcatg gggtagcccc
     4201 tgaatgtggc ttaccgattt aatgacataa tcggaagccc ccattttcat tgcctgtata
     4261 gcaatttctt ctgaacctgt tcctgtaaca ataatcacgg ggacaccggg ctgaaattct
     4321 ttaacgaact gaataacctg caatccgtcg aaaccaagaa tattgaaatc gctcagaaca
     4381 aggtcgaatt cttcattttt caaataatgc tcgaatactt ctctgttatc ggcttcaatt
     4441 atttcaaact tatcgtcttc cctcaacagt gcatctttta caagaagccg gtcgtgcatg
     4501 ctatcatcaa tatgaagtat tctgattttc ttttccatgg tcaataaaat ttaatttata
     4561 atccgggggc accgatattc aatgtaaacc aataatcatc aatttttttc acaacttcgg
     4621 taaacccatc aaatgaaacc ggtttcagaa ggtaactatt tgccccgatg tcgtaactta
     4681 atgccctgtc gccatcttct ttcgatgaag tcagaattat taccggtata cgtttcagaa
     4741 tatcagagct ctttatttgc cgcaatacct caaaaccatc gatacccggc attttcaggt
     4801 cgagtaaaat aagattaggt aatgggaact ccgacctgtc agaaaattta tttcgtccga
     4861 aaaggtaatc gagggcttcc tgcccgtttc ttgctacgct gattttattt ttaagttttg
     4921 cttccctgaa agcatcgaga gtaagaatta catccatttg attatcttcc accaaaagaa
     4981 ttgttgccaa attttccata tatttagttt ttacaaagcg gtaattcaat tataaagcga
     5041 ctcccctctc caaccacaga atcaacactt atcgtgccat tgatcattgc cacagctttt
     5101 tgtactgtgg cgagcccgat accggtaccc ggatattggt cttcgctgtg caggcgctga
     5161 aaaatattaa atattttctc ccaatattct cgcggaatac caataccatt atcggccacc
     5221 gttacagttg caaaattgtc gctggtagta caggatatgt caatttgcaa catcacatcc
     5281 gtacgcctgt aagttatggc attttcaatc aggttgttaa aaatctgcat cagcaggctt
     5341 tcgtctcctt taacttctgg gaaagatgaa tctgttctga gcactccccc gacttcttcg
     5401 atgcgttgtt tgaaatcggt ttttaatttc tctgcgattt tgtaaagcga aacagggtgc
     5461 aggtcaatgg atttccgacc cagtctggaa taattcaaca aatcgtttat taactgctcc
     5521 atcctgatac tggcttccac aatataattc atatattgtt tcccttcctc attcaaagag
     5581 ttggtgtgcc ttcttgaaag tatctgcgaa aaaccaaaaa ttgcacgcaa aggggccctc
     5641 aagtcgtgag aaatagagta actgaacgac tcaagctctt tatttatagc caccaactgt
     5701 gcggtgcgtt cagtaacttt gtcttccaaa gtaaggttta gcagatgtag ctcacgcgtt
     5761 cgttcttcaa cgcgcatttc aagttcgcga ttatgctttt gcttgtttct gtaagcaaaa
     5821 taaataagcg caaccactgc aaacaccagt aaaataataa ttaacaacag atataaaatc
     5881 ttccgctgat tatgaatttt cgaaccttgc tccttcagca ctttcttctg agcggcaata
     5941 tgtgtttttt gtttttccaa ttcctgctct tgttctttca acaactgggc accttcttcg
     6001 agcgaagctc tttgattttc gaggtctttc gattgtaact taaatgtatt attctgctct
     6061 acaatctgca atttctggtt ctgcagttca gccgtttgct tacatagctg gctttgctga
     6121 tgttccaaca ccaactgttg ctcctgaatt tgtttggatt gccggcgcaa gctatctcta
     6181 cccatttgta cttcttcatt cctttcgatc acctcctgct gcagtttgtt caaattgttt
     6241 tcgagatttt ctgctcgttt ttgcaaactt cgcaggcttt cttgcccttc atggtacaaa
     6301 gcagcaacgt caacttcagt ccctcctgta agcaccatat ccggcataat ggtgatattt
     6361 tggttcagaa tatttgatct gttaatttta aacagcagcg taccgtcttt tgaggtataa
     6421 aagtcaacca taattaggtt ttcatcctta tattcatcac tgacaagtaa aatattttta
     6481 ccttcaattt tatcgaaaat atccaccaac atttcttcgc tgtcgttggt aacaaatatt
     6541 agctggcaag caacgccggg cttgaaagtg gttgccgaac ttacagtaat aggtttgcct
     6601 ctaagggttt tggtctttgc cagattgttt agttcattta ttatcttctg gtcctgccct
     6661 attatcaaaa agtgaaaatc agatattacc gtttcgtttt gccactccac attttttgca
     6721 aaattataga tatatgcact cattgccgcc tcgcgcgata cttgttgggc ttgcacatac
     6781 attgacatca gggtaaatgc aataaaataa aatacactat ggcagacatt tttcattctt
     6841 ttcttttaat agtgtaatac atatgccacc gaaagaagta ttgtacgttg tggctgacga
     6901 tagcgatcag gatcacggtt cgatgtatga taatactcct gattaagaat attttttaca
     6961 accagttgtg atgtaaagtt cttataattc aatagagaaa aggctccgtc gaatactata
     7021 aatgcatcaa caaagttgct tccggtagat tgaatagttt tgggattttc gcgttgaccg
     7081 acataatgag ctctgagatt taagctgaaa tattcgttca ggaaataact gatcatagca
     7141 tttgtacaat gcgggcttat ctcagcaatc ggatcggaat aagaacaact gttagtaaag
     7201 gtgtaattca acgacgccct gatttttctt gaactatatc caaagctagc ttctgcgcca
     7261 cgaatattta tttcaccgat attgtaccag cggtaggcat ccccaatata ttgcttggta
     7321 attgcgtttt cgagtttgtt attatatcca gtaaccgtaa agtttacttt gcgtgtaata
     7381 ttaaaaacaa acgatagctc gagcgactgc atcttttcgg gcattaaatc agcgtttcca
     7441 aggccatcgg tataatccca aggtttgggc gcccggaaag cctgagcata cgaaaatcga
     7501 aataagtgcc ttccaagcac atagctgaca ccagccctgg gggtaagtac ctgattataa
     7561 atactactgt agtcgaagcg cactccaccg gaaatatata tgtttttaaa taatgttagc
     7621 cgtggttcca caaaaacact ggcgaggtaa ttgtttaaca aattaggtct atccggtctg
     7681 ggaggtttta gaacagcact gtcgctgtat gaatatgaag ctttttccga caggaattcg
     7741 tactcaaaag tcaatccgcc tgttactgca aaaaaatcac aagctgtata atggagaatg
     7801 ttttcgaacc cggtaaggtt gttcggacga taataaccaa cctgagccgt gtcgagaacg
     7861 gaatataccg agttgggcat tacggttgcg ttgcggttat atagcaaaga actcaaatgc
     7921 aatttgggtg tcagatttcg tgaaaatttc aggtagttgt tcaaaaagca gatattccat
     7981 tcggtgccgt aatcacggta aatggttcct accgacttat tatatgtcgc tgtagaagct
     8041 cgtttgttaa tgaaacctgt tccgaaagta aagtctttaa attgaatttt caggtcagca
     8101 gcatagtctc tttcgaaatt gtccatcaaa tctgtccagt tattatctcc tgcagcgccc
     8161 ttcaaatcgg ctttatcgga attcttcaac attcctgaaa cagtaatgcc gaagtttttt
     8221 tccgggttgt aaaacatgaa atttacattg ctttcatacg tgttaaagga tccaaatccc
     8281 gatttgatct caaatttatt ttcagtgggc gcttttgtaa taatatttat tattccggta
     8341 acagcgtttg tgccgtatgc aacagatgcc ggacctgcta taatttcaat ccgttccaca
     8401 ttcgacaggt tatactgagc accgccataa aagcctcctg aattaagttc gttaaccatt
     8461 ataccatcaa taagcagaag cgtaagattg ttctgattgg gaattcctct ttggaataca
     8521 taactattaa tgccgagtat atttctgaac tggaacccgg gcaggtcggc aagaacctct
     8581 tcgagcgtaa aatagccatt ttgtttaatc tgctctactg tgataattat aattgttgaa
     8641 ggtacttctt taatttcctg ctctgttttt gaggcagtaa caattttaag tttcgacaat
     8701 tcggtaaaat ccatatcgta aatatcgact gtatcttcct gtgcagaaat aataccggca
     8761 ataatcagaa aaaataatat taaaccgaca aatattgggc ttttcatctt cgaattatca
     8821 tgctactccc aaatgtagca attttcttcc ggaaataccc gaggagctat accgattagc
     8881 aaaaagaaac catgtgaaat cagcatcctt ttttgaacct cttgatatta cacagttata
     8941 caatgccaag gtttttaata acatgaacta tactaactta aagtatctat gttacttttt
     9001 ataaatttta ccctccttta gttaaagaaa cgtacttgca ttttgaattg aaatgtacgc
     9061 agattgcaac tccgttgcgg tgtaatttct catattttga ataacacaaa ccaaattaaa
     9121 ccccaaaaat gctttgattg attcagacca tctgattaca ccctcttgca tcgctattat
     9181 cggcacgccc aagcagctca aagctaccgt cgctttgcac cgtacccaca tcagaagttc
     9241 caataaaaca acaggaatga atattggcta ggtcgatgat attcaacata cctgttccag
     9301 cttcagaaat acacagaggg tcggtagcat cacgcacaag tactttcata cgaggggtcg
     9361 gtctgaatat tccatcagaa acagcataag cctgcgataa taattctgtc attccatatt
     9421 ccgaatgaat tgttttcacc gagaatgact tttttagaaa accatgcatt tcggaacgaa
     9481 taatttcttt acccattcct ttagaacctc cggtttccat cacaatgcaa tgctgaaccg
     9541 gcatagggta ttgttcggca aatttcatca gggcatggct tacggcccac agaaaatagg
     9601 gttgtgccac ggcttccagt ttctgaattt taagaaacac ctcatcatcg ccactggaat
     9661 atatgccaga ctcactgtgt ccacctgctg caatcaactg ccgaagcata taaatgagcg
     9721 aagcatcgtt tctttcgata tagccgggca gaatggcaat aaacctaaac tgagccggag
     9781 caccgtaaaa acttcggaac ccctcatcta ggttacggtc gtataaattg aggtcgtgta
     9841 cataatgata cgaattcgtg ccagttgtac ctgaactttt gaaaacagat gtacgatgtt
     9901 tttcgccaca aacaatctga ttgtttttaa aaaactctat cggtaaaaat ggaatattgc
     9961 ggatttgtct tacattatca ggcgatttgt gcaataattc gcaatacctc ctgtaaggct
    10021 tgcagcattc atattgaatg acaaaagcac gcattgccaa agtgttgaat gagtattcaa
    10081 aaagcttgtt gaaaatatcg tttttatatg tttctattga atcggacata aaaaaggggc
    10141 tttcgcccca aaattatgaa atttagtgca attatttttt ggtaaaacca acactggttg
    10201 catcgtaggc gagaatatac ttacccttag gcagagaact tacatcaatg gcctcgccgg
    10261 aaccttcgag cactgttgcc ccaaaactgt ttacaacata gtacatactt gcagcactga
    10321 gtgtgatttc tgtttttacc tttttcagct tggatgtaac cacaacaggc tcctgggtat
    10381 ttgtgtacaa atattcatca gaaaaccgtg tgcggtacaa gtgatctttc tgaatgaccc
    10441 tgtatcggtt ttcaccggat gatggaagaa tggctgctgt ataggtattt tcgccggggc
    10501 ctcccttacc ctcaactgtg gctacggtaa tccagcggtt ccacctgaat tgttccacct
    10561 gaaacggaat tttactggtt tcacccgtgg tgctaaaatt tagtacattt cccgtaatat
    10621 tgatggccgt aactgtaaag gtggttattg gtttaagtac ctccgggtta attatcttcg
    10681 gtgcacaatc gttactgtga acaaacctga tttcgagtcc gtctccaact tttaggtcca
    10741 gtttcgacaa cgaaatgcaa aaagcactcg aattcagcac atcttcataa acattgttgt
    10801 ttataaaaat ttctgtcacg caataggtgt atccctcatc atcaagcatc gggtttataa
    10861 cataaagatc gattccctga tacaaacctt taacaatata ttcttcagct ttcaaattat
    10921 aaccacctgt aaatgtgatg attacactaa taaatagtaa atattttttc attattaaca
    10981 atatttatta acaaagttat taacaaagat aatgtacttg ttgcgagttt gcaaattttc
    11041 ttgacaattt tttcataaaa tcatatttat agcatattgc accatccaca atgctaagtt
    11101 aataagtgat ttaaaatcag ccttcaacag tattgattaa agcgtttgat tacttacatt
    11161 tgtaaaaatt gcattatatg aaaaacatcg cattgcttat tacagcagtt attttaagta
    11221 atttcatatt ttctcaggag ttcgaaacca ccaccgatta ttacaaaaat ggtgttaaaa
    11281 aatcggaagg gatgatgcat ttgggcagag aaatgggcca ctggcaatat ttcgacagca
    11341 acggaaagct ggtaagagaa accgattatt ataaaggaga actgtatggc aacgacatca
    11401 cctattaccc caatggcaat aaaaacgaag agtttacatt tgaaagcaat ttaaaaaacg
    11461 gttcttacaa gaactggtac gaatcggggg ttttacgcac cgaaggtttc tataaatacg
    11521 atgtaaaaga cagttcctgg acaacctact acaatacagg aaaacccgaa atggaagttc
    11581 tctataatca gggatccggg cgaatcatat cgttttactc cccaaaaaat gaaaaaccgt
    11641 tgcaaatggc aatggtaccg ttttgcggta ttacgagttt ccttccaaat tatctgaagg
    11701 gtctgttgcc gatggtttgg agcaaggtct ttggacatat tatttcgaaa atggaaatat
    11761 taaagaaacg ggcactttcg acaatggaaa ccgtatcgga acatggaaag gatattatga
    11821 aaacaaacaa ctaaagagcg atataaacta cgaaaacgga aagtgcgtgt attactacga
    11881 aaacgggaca aaagaaatgg aaggaatcat gaaaaacaaa gagcgcgatg gcgaatgggt
    11941 tttctggtat cccgacggaa acatacgcag cagaaataat tataagagtg gtgtgcgcaa
    12001 cggaatacag gaaaaatact atagcaacgg aaatctagaa gcacgcataa attatatcga
    12061 tggtaagaaa aacggcgaag cacactgggg ccttcaggac ggtaaaaaag atattgacgg
    12121 aaactttgta aatgatgtgc aggaaggtct ctggacgtac tggcgcaccg atggaaccaa
    12181 agggaacgaa gggtattacc tgaatggtga aatgagtgga aaatggacgt attggtacgg
    12241 caacggtcag gtgtggcgaa taatcaacta tgaacatggc cggaaaaatg gcgactacat
    12301 tgtgtattac gaaaacggga atgtgttccg taagggtcaa ttcgaaaatg atgtcgaaat
    12361 tgggctatgg acagaatact acgaaaacag cgcaaaaagc ctggagggta attacgattt
    12421 cggtgtaatg accgggttat ggaacagctg gtacgataat ggtgaaaaga aatcggccat
    12481 tcattataaa aacggactca tcacagacaa agccctgtat tactatccca acggaaagat
    12541 tcagattgag gaaaactatg tagtttacga aaataaagga aatcccgatt tatatggcga
    12601 atcgcgggaa gtttcggtgc tgaacggggt tacacgtaca tataacctaa aaggaacaat
    12661 tcttatggaa ggcaattatc tgcttggcaa aaaaaccggc aagtggacat attataatcc
    12721 gcaaggagga aaaatcaggc ttgaaaatta taaggacgat aaacccgatg gcaaatggca
    12781 ggggtggtac gatcatggcg ttcaggaatt tgaaatgaac tataaaaacg gacttctcga
    12841 aggtcgtatg gttactttcg atcagcaggg caatgtggaa tacgatgcta tttacagggg
    12901 caattacgaa gttgaggtaa aagtaaataa taacgaccca caaaaggaaa atcaacgcga
    12961 cgattatcgt cggtagatat tcaaatacag attgcttcgg gttttcaaca ttcccaatga
    13021 cgggctttta aacgtcattg cgaacgcagt gaagcaatct ccgtacccgg agacgttgca
    13081 ataaccaccc gtcgaaatgc agattgcttt gtcgcacttg ggtgctccgc gcaatgacgt
    13141 gctctccaaa acgtcattgc gaacgcagtg aagcaatcta ccggtacaga gacatgttta
    13201 taaccgaccg tcaaatgcct gtattgagcc tgtcgaattg cagattgctt cgggtttcca
    13261 accctcacaa tgattggccc taagttctaa aatcctatct acaattcgaa atgctcttcg
    13321 ctgaaacgga ttccgtaagt ttcgagttct ttcagtattg gtccatataa atcgggagtg
    13381 accggcacat gcacaccggg ctttgtaaat gcttctataa gcaccatttt tgtggcaatt
    13441 gctgccggaa tgcctaccgt gattgccata gctgtatttg cctgatcttt tccgataaac
    13501 accatcgacg attttattcc gtaccgtttg ccgtgaagtt catattcaaa ctgatgctgc
    13561 attacgatca tgtctttatc atcggcacca agtgcccact tctcttcgag cagcttctga
    13621 agtatttgtg cgggagtagc gtttttcata ttgatacgcc tttcttcgaa aataccgagc
    13681 cattcgagtt ttttcatgat ttcgccattg cgatccaatc cgagaaaatc gcagagttta
    13741 tcctcaacct ttgcattcgg gtcgaaacgc aaatacgaat taataaaatc acggtaggtt
    13801 gcattttcgc ttccttcata aatgtaggta tcgtcggtaa taccgagctg tacaaacaca
    13861 ttccatgctt cgcaataccc cggacgtctc attgtgcccc tgaaaatagt cgggatatca
    13921 atcagtccgt aggtttcgcg gtacgacagg gagtcgcggt ttgcataaat ttcaaattca
    13981 ccataaccag gaatttcggt attttctacc ctgcgatata tgttatgata tggaatgaat
    14041 ttatatcggc cattgcgtat aaaccgtgct gtcccctgcc ctgccagaac cacattacgc
    14101 ggattccaag taaatttata attccaagga ttatcatcgt aatccggagc aatcagtcca
    14161 ccggtactcg atttgaacga aagcagcttg cctcccttgt ttcgtatctc gtcgataaca
    14221 tgcattgccg acatgtggtc aagccccgga tcaacgccta cttcgttaat aaaaatgagt
    14281 ccctttgctt tggcttctgc atcgagcgac ctcatttcgg gagaaacata cgatgcggta
    14341 accatgtttt tgccatgttc ggcacaggct ttggcaataa tcgggtggaa gcgtgccggc
    14401 aaaaacgaaa ctgccgtatc gcactgactt acatattgct gcaccagact attatcgagc
    14461 acatcaaaaa aaacggaacg cgcattcgga tgtgctttta tttttcgaga ggcaactgcc
    14521 ggatcgctgt cgcctactat aatctgccaa ttatattttt cactgccttc gagcaggtac
    14581 tcaatcagcg aagttgcgct tagccctgct cccaaaatca atactgtttt catcttaaaa
    14641 aattttaacc cgctaaacta taatttatat ctctgctaaa aaagctattt cccatttttg
    14701 attatggtta aaaacacata tgtgcttttt gttggcaggg aaattgtaat tttgtcgtcc
    14761 aaataccata atgtatgaag caggttgcag tttttttatt ggcagtattt tcagcccttg
    14821 ttatgcaggc acaagatgta acgggcaaat ggataaccgt cgacgaccag acaggaattg
    14881 aaaaatcggt ggtacagatt tatgaaaaag acggaaaact atacggaaaa gtaattgcta
    14941 ttatcgacaa atcgaaagga gaaaaccctc tttgtacaaa atgctcaggt acactaaaag
    15001 acaaacccat tctgggaatg attgtggttc gcggtctcag caaaaccaaa gacggttggt
    15061 ttgcaaagaa tgcttttctc gaacccgaaa gcggctggat tgttgatggc aaactctggc
    15121 tcgagggcaa cgagcttaaa gtacgcgctg atgtgggaat atttcatcgc acacaaacat
    15181 ggaaaaggtt acggtaaata aactgtaatt gaatggttag tgctgcaccg caccaatatc
    15241 ttcaggcgta tgcttgtgtt taaaaagtaa tgcaaagagt attgccacaa taaaagcata
    15301 aatggcaaag ctgatccaga ttccctgcca ttgcttcatt ccgtcaatgg taaaaaattt
    15361 gtcgataaca agcccgctgg tccagctacc cagtatagct ccaaacccgt tggtcatcat
    15421 cataaaaact ccctgcgcac tggcacgtat tttcgaatcg gtttgggttt caacaaacaa
    15481 cgaccccgag atgttaaaga aatcgaatgc cattccatac acaatacagg agagaataat
    15541 catccacaac ccactttcgg gattgccaag tccaaaaagt ccgaaacgca acacccaggc
    15601 aaacatactt atcagcataa cttttttaat accaaagcgc ttgaggaaga atggaattgc
    15661 caggataaac aatgtttccg acatctgaga aatggacata ataatggtgg aatatttcac
    15721 cacaaacgag tcggcatatt gtggaatatt cttgaagtca tcaagaaaca cgtcgccgta
    15781 agcatttgtt aactgaagtg cagctcctaa aaacattgaa aaaacaaaga acaatgccat
    15841 ttttttgttt ttaaagagaa caaaggcttc caggccaagc atctgaataa aagttttctt
    15901 ttgcgatgtt tgggcaaacg tagggcactt cggcaatgta aaagcataaa tgcccaatag
    15961 taatgaaata gccgaagcaa taaaaaactg gttttccgag cttttgttac cggtcaggtt
    16021 gacgatccac attgcggcaa taaaaccaat ggtaccaaaa acccggattg ggggaaaact
    16081 tttaacaaca tcataattgt tgcttttcag tgccgtatag gcaattgtgt tggtaagggc
    16141 aatggtgggc atataaaaaa tcattgccag taaaataccc cagaaaaaca tggttggttc
    16201 gttgatctga gggattaaaa acaacgacac cgctccacac agatgaagaa taccatagag
    16261 tttttcggca ttcacccact tatcggcaac aattcccatt aacgcaggca taaaaagaga
    16321 agcaataccc attgtagaaa aaataattcc aaactccgca cccgaccatc cttttgtccc
    16381 gaaccagtaa tttgcaatag tgatcagcca cgatccccaa atgtaaaact gaaggaaatt
    16441 cataataatc aaccgcacct taagacccat atcaacgtga tttaattgtt gttaatcgat
    16501 atcgcaccaa taaaaaagta actacacatt ttttcgtttg ttcagttcat cgcggattct
    16561 gcttgccagt tcatagtttt cgtcacttac agcttcattc atcatctgct ccagttctcc
    16621 gtcggaaagg ctgaaaatat ctttctgcgg gcgttgctcc ttctgtggct cttccgttgc
    16681 tttgggaccc tcatcatcgg ggagattgag gatgattccg gctttttcca caatttcgtc
    16741 ggttgtgtaa atagggcatt taaaccgcaa cgccagtgct accgcatcgg aagttcgcga
    16801 ttcgataagt tccagttgtt ttttgttgcg gaaaattaac tgtgaataaa acactccttc
    16861 ttcgagctta tagataataa cttcttttaa ctctatttcg taacgctggg caaaatgcag
    16921 aaacaagtcg tgtgtaaggg gtcgggctgt gattatctct tcgagctgta tggcaattgc
    16981 ctgggcttcg aaactgccga taatgatggg aatacgctgg tcgctgttct caaccgatag
    17041 aatgagcgca taggctccgg cctgcgtctg gctgtacgat aagcccaaaa cctgcaacct
    17101 gacttttttc atctttcggc atttgctttc atggcaaata tacaaatttg attttttaaa
    17161 tttgcctcaa caactattta taattatttg cgccatgaaa ttaaaagcac tttttccgct
    17221 aatttttgtt ttcgcccccc tttgtctttt cgcccagcat aaaaacagcg aaacagcacg
    17281 ccattttacc gatctgctcg aaaaaggcaa ttacacagaa gcttcgaaac tgttgcaggc
    17341 aaaccctcct accgacagtg ctttctgggc aaatgcatgg tcgaaattca ttaaaaacta
    17401 tgggcacatc accgaagaac ggagtcttga aagcgatatt tttggtaaat acgacatggt
    17461 gtaccgaatt gtggatacgg aaaaaaagac gattaaactg aattattcat ttgagaacgg
    17521 aaatctgacc ggattcaggt atcaggttta cgacactgca aatgcacatc acgctattta
    17581 tgccaaccca ctgttcaaag agaaaaagct ccgctttcag tcgggaaatt tcagcatgtc
    17641 ggcagcatta ttggagcctg cagttaataa agactcaatc attgtaattt ttgtgcatgg
    17701 gtcgggctca accgaccgcg acgaaagcgt tggcggcagc aaggtttttc tcgatctggc
    17761 acaggggctg gcttcgtatg gtattgcttc gttccgcttc gacaaacgca gctatgtgta
    17821 ccccgaagct ttttcgaacc gcagctttac agtgtacgac gaaactgtgc aggatgcggt
    17881 ttcggctgtt gactattgcc atcatgtgct gggatatgcc tattccaaca tctttgtgct
    17941 ggggcacagt ctgggcggaa tgcttgcccc tcgtattgcc aacagcattg caacacccgt
    18001 tgggggtatt ataatttttt cgggcaatgc acgtgcactt cagacactca tcggcgaaca
    18061 aacagaatac ctgatgaaac ggaatgggaa acttaccaaa gaagacaaaa agcgccttgt
    18121 aatgatgaat gagctgaaca acaatgtatc gaagctccaa accggtgatg cggcaaaaaa
    18181 cacgttggca ttgccttctg gattacccgc ttcgtactgg tacgatctta aaaattacga
    18241 tcagtgcaaa accgccgctt ctcttgatat tccgattctt gtgatgcagg gagggcgcga
    18301 ctatcaggta accaccgaag attttttatt atggaaaaaa gccctgaaag gaaaagcaaa
    18361 tgcaagcttt atgctattta ataaactaaa ccatctctac gttgcaggcg aatccaaatc
    18421 gtatcctgaa gaatatgaga agcctggaaa tgttgataaa tcagctattg aagcaatctc
    18481 cgactggttg aagttgcaag aaaaataaaa atcaacagca cagaaaaggt caattgcttt
    18541 taaaatgtct aatgaatgtc tattctaaca tttgacattt gatttataac atttgacttg
    18601 ctccggctaa tcaaccctta taaggttgta gcggagatac cagtcgtaat aatcagtacc
    18661 aaattcaacc aatgaaaaaa acacggcatc gcctccatca gagaagctta ggtgctgtat
    18721 ttcgaaatta ttactgcact cgtatgaata cgggtaggct gacatagcat attcgtaccc
    18781 cacattaagc actccatatc ctttgttgtt gatgacttcg tctattgtaa aggtctggca
    18841 tggaatgtcg gcagaaggtt caatacacat ctgtcctccg gcagggttgt caaccatttt
    18901 gtatccaaaa taaaaagggg atggccaata attggggttc gatacattgg aggctgtagc
    18961 aatataatgt ccggtttcgg tgaatgtgat atcaatattc caggtggctc caaacatagc
    19021 ttcagcagtg ccggtccaat gaccaa
//
LOCUS       DAOOQP010000050        18988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3536_length_18988_cov_5.355517, whole genome shotgun sequence.
ACCESSION   DAOOQP010000050 DAOOQP010000000
VERSION     DAOOQP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_18988_cov_5.355517"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4267
                     /locus_tag="PLK75_06835"
     CDS             <1..4267
                     /locus_tag="PLK75_06835"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOY38999.1"
                     /translation="NSNTLILFDDHDDGSIPYTILRPTDPVAQFMGTPQLSMLNGSEQ
                     VYVPRQRYGAGTIARWNPGAKILVYDPTQANVPVLNSDLSNAAVLMVYGRGFDDDDRG
                     WVMYEAAHTLLKGTTSDGPAIRAFFNFSFFAQIERAPQIPSNAIDGISPNGYYQSGEQ
                     IHLSVDAVAGLSTTSIYSYTWMVAVDYGEYTTTGFDPAYSTTSNSTTYTVPYTPTIAE
                     GLVSPAIIIVIVSDNCGRTNTISVPVNFIPNPLPPIPVDDEYTLPGSCDPGLSLSFNV
                     MENDADAQESTLSFYSLNQATATTTPAGDVTWVDNGGGNITIYPDVNYNGVATIEYTI
                     INEFDSVASATITVTIGSIDADGCLSNQFWGVNEYKFITNDQLVSSTTPPGATTTGTQ
                     LDDYESGYQNGTYLDFQKAYTNNIILAIGSEQSLAERDIVMLNTRTQNGSGTISIEIG
                     QSSSGPWTDAQTFTINDTESIKNYTIPTGVTDITHIRISAGNITTPANSANKVYLSAV
                     RFYDYDCVSRAPRATNDNITILEDLPTVISVTANDYDPNGLNFVLKNIITSPSHGHVS
                     INTDGTITYVNTTDYSGTDSFTYQVCNDSGYCSTATVTITIVDDGCSSGSYKPTSGSG
                     TITKIFQYQYSGTNAATANLTSDNFPDSYIKQDRPNENKGGSSAKLEVGKKYSGTKAR
                     RALFYFNVSEIPTDAIIQTATFSALRTAGDGVSQAINLHQITQDWSESEVTWNLAYSE
                     HNWTSAGGTFGSVLSSTTVLANGRYYWDVLNSVQAWVSGSSSNYGVLLKTGETIDKKH
                     VFASREDGTVSKRPSLTVTYVVPASCEAIPNRNPLANPDYSETYSSAADTIAVLDNDT
                     DPDGNSISISGIGTVTHGSAVISGNNIIFTPTYGYNGIGTVVYTIKDNGSGELTDQST
                     IYVQIKNAPTVAIYDVDSVDSNDELVIYVIDNDEDPEGQTSTISQIVTQPSNGTVEIS
                     GNTLVFNPYPNFTGRDSLVYEVCETYVEECDATTLCDTAIVYITINNRPPEIVPPLID
                     TVVPCFCSVINLHNYVSDPEEGSLTFTIVSGPTPPGGGKAGSGTLENNGDGTVSYTPP
                     IPLDALEYITFTFAVSDDGNPTKTSDTVEVTFRIVPKDNTAPVAADDDWLDAVMNTDA
                     NVSIGDNDYDPDGNPLSLPLLITQPLNGTAEILPNGLLRYTPDYQFYGTDSLYYEIHD
                     TITPSSQTCQNEKGTSDTAKVRITINYDPDQFLPVSLVLYEAKCNNGVTEIDWATVSE
                     SNNDYFVIERSKDLVSFEEIARVNGQGNSNDYKFYRYFDRNAASGTNYYRLKQVDYNG
                     RLTTYTTVNSSCSSSVSGELMALVYPNPFNTQLNVSIENVVEGEVVIELFDVAGRKLF
                     GLNEYNYSEIYFRELNIDNLVTGMYYLKIHINGQIITKKLEKK"
     gene            complement(4574..6382)
                     /locus_tag="PLK75_06840"
     CDS             complement(4574..6382)
                     /locus_tag="PLK75_06840"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOY39000.1"
                     /translation="MTILEKILANHSNEKNVKANDIVDIFIDARVARDFGGANVVKNL
                     VDEGLQVEDASRTFFTFDCNPTGSDQKYAENQHKCRLFARANGIAVYDIDRGIGTHLA
                     IEKGLAFPGSTFVSTDSHANIMGAIGSFGQGMGDQDIAAAWANGSVWFKVPESAKITL
                     KGKRPANVTAKDIVLNLLARYGANTLLGYSVELYGEAVDKLTLDERITISSMATEMGA
                     IIIIIPPSDEIIRYSEKATGKKIEKITADSDAVYAFDETIDIEKFVPMVSKPGKPHDS
                     VDVSSQYGKKIDSAFIGSCTNGRMEDLRKAAAILKGRKVAPGVVLKVVPATDSIWNQA
                     LDEGLIQIFKDAGAMVSNAGCAGCAAGQVGQNGPAEITVSTGNRNFPGKQGKGEVFLT
                     SPDIVAASAVAGFITTPDNIPAKPAEFAKPDISAKKTGNDAKKTTGPKPKIVEGKAWF
                     IHEDNIDTDMIYHNRYLTITDISQMGQYTFDNLKGYEDFAKKAKTGDIVVTQKNFGSG
                     SSRQQAVDCFASLGISCIVAESFGAIYERNAVNAAMPILTYKNIEALNLTEGDTIRVN
                     FETGLIENLTKSTSVQANPFSKVQMEIYQRGGIFNK"
     gene            complement(6599..9598)
                     /locus_tag="PLK75_06845"
     CDS             complement(6599..9598)
                     /locus_tag="PLK75_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39001.1"
                     /translation="MIRVICLIVLWISSATCFLLSSTTILFQGFGAVQAQNPESPVVH
                     PKSAFVYLGQIPEGVTCTTVPNPMGNSIGRPGGFRINYNFTGWPQNTYWGLDEVQSVL
                     NLNNNLPPNQNCSFLNLTYDASNAETNLSQGKICFTGQQSYYCLNTSQQWINKTCNVR
                     MRVVVTYTNLSTPVPLSLQSGIITGVPSASFVVRVYMEVLTSNLDNVVWVGTNYYNYG
                     GWYGVIDVYNALATNPSRSICTTFDTQSFYYGNLSAEVEPEANPGGTSITIIPGSGSN
                     ALGTAVGLQVIYNNANWPECINWGLYKNQAVLNLNNTQPPNYGCNFLTLVFDPINTET
                     NLVNGKMCFTGVQSYYCLNASGQWISKSVHVRMRVIVTKSNGTTPIPLGFVDDYILMH
                     VKENFIAKVYIEANASELDNLLWTGTNYYSLGGWRGVTDIYNSLHTYMNYSICTQFNT
                     NNFLSFNTVVSASSNSPIHLGNTLEFTGYSSMDCCYHWSGPNNYTSEEQNPIINNTVI
                     QNFGNYELFITDNIVCYGYDEIVTMVLPSVSVSPEDGEVCQGGSFLLTATAVGTGYTF
                     TYQWYYFINGVWKIAPGFTQSTLNTNAPYTRQYRVKAIGNNGLGTVTVTSDPVTVTVY
                     PDPAITLQPLNRNICTGGTHTMTITATGGITGLNYQWQESAEESNWTNLAEETSNSLT
                     ISNATLTRYYRCVVSAEGPGCNTVISNSALLTIYNDPQILINGNYEFCAGESAFLIAT
                     AVTGGCGVRSYQWQYYDDGFWRDMDGETNYKITVYPSVTTQYRLALSTHGNGCNTAYS
                     TPVTVTVHPDPYITTQPEGGTILAPGEEFPIAVVADGGMGLLYQWQYFMNGIWIDIPG
                     ANSPEFVTQAPYTRSYRVKISSNGAGCDYVYSNIATITLGGEKDNFQENDPGSLFSML
                     LPPEVLIFPNPFSDYIIIALSNLPCNEKLRIEVFDLYGRKITEIAENQVSDTYNKNLS
                     LSGLTPAMYFVRITSGKNVLIKKIEKR"
     gene            complement(9601..11670)
                     /locus_tag="PLK75_06850"
     CDS             complement(9601..11670)
                     /locus_tag="PLK75_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39002.1"
                     /translation="MKKLYLVLFAFLSFLCNPVSAQTPENWSVNYVPRYRCINDIHFW
                     SPFQVSIVGGNPSNDSILYWTYSSNGGAEWDYFGDHPMNPMLNSAVFINEHIGYAGGM
                     NEVLYKTENSGKLWFPVALNISLNHADINKLCYSDVSGIWAAGGLDGNSAFVLNSSDL
                     GNSWNVAGEWGNNELISMAVSPDQTVYICGTNNFLKYNDETKTNWADVNISSLNGTVD
                     FTSLFFYGNIGFCTGGTRGSDSTRVVLKTIDGGVTWLCILEEAGPRLNCISVIDENNL
                     VAAGDYGTILKSSNGGETWQSIDFSEITYLDFYSVCFTNTHLGAVGGNFGHFIKYYDG
                     SPLVATNNATNVGKYKATLNAQVQSVNESCTAFFCYGTDENTEHLINMGEIQGSGISN
                     ITYDLSELSINTKYFFRVKLTDGNSQNYFGELKSFYTGNPIPNWDFEIWDNTLPTGWF
                     YEYSYLPVEEMPPLPFSKNTIGHLSPTGINIQEVISEGRNENIEGEIYTDLFPVSVRH
                     TTLEGYYTYYSDTQDTAKVSVMLFHGDDTVGTGSFNIFLQYDYYVYDWTKFIVPIHYT
                     DENTTPDFAKITIRSTSKNNPAASTLGIDKLGFDGDFIPVLQVSCYSICSLYPNPCSN
                     FVRIKAPVEYHHFTARIYNFSGKLLQEKSFDGNLGEMFTGELSSGLYVIEIKSETVTQ
                     YSKLIVK"
     gene            12017..12556
                     /locus_tag="PLK75_06855"
     CDS             12017..12556
                     /locus_tag="PLK75_06855"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOY39003.1"
                     /translation="MTEPMENHPSLSKHVVEFLLVAKEYVSFSETPQHFQQSNYIDTC
                     LKLFSMLYLKALFLPDPDEKEKPDEYGLEFVNAMQYERIKANFEQQLGEADAIVPFRD
                     AGMINTDDYVNTSISELLADVYQDVANFVEAFRIGEPQLMDEARVKCSLNFKLFWGPR
                     LIVVLDQLHRFKYIENNNN"
     gene            12561..13163
                     /locus_tag="PLK75_06860"
     CDS             12561..13163
                     /locus_tag="PLK75_06860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY39004.1"
                     /translation="MLFESSISPEAVQALPELKFEGDIIVVDTLQQLKKLVPELILEN
                     TWGFDTETKPNFLKGNPNPNTVSLLQLSGAGKTYLFRLKKIGLPDIIKHILSSPKIVK
                     AGVAVRDDINALKKIKTFSPEGFTELQTEAHKFGITDISLRNLAAIVLGMRISKAQQL
                     SNWENEQLSEKQIYYAAIDAWVCRQIFLKFQASIHDRENS"
     gene            13144..13548
                     /locus_tag="PLK75_06865"
     CDS             13144..13548
                     /locus_tag="PLK75_06865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HOY39005.1"
                     /translation="MIEKTVNYRVIYGDTDAMGLVYYANYLRFYEIGRSEYMRSLGFP
                     YMNLSASGIECPAVEVRMKYHSSAHFDDLLTITTSLKEIPKSTMVFVQKVFRENQLLN
                     EAEIQLCFLNMLTHKPVRCPGVLRDYFKNMGY"
     gene            13608..15980
                     /locus_tag="PLK75_06870"
     CDS             13608..15980
                     /locus_tag="PLK75_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39006.1"
                     /translation="MKIKFLLFLILAFVSMLGYSQKYTISGYVEDKSSGEKLYSANVY
                     DMRTKAGAITNEYGFFSLTLKKDSVDLVVSFVGFTSYSFRFYLDRDIQLNIKLDPVIQ
                     LAEVVITDKKADQVVKDVQMSVIEMPVNQIKTLPVFMGEADVMKTLQLMPGVQSGSEG
                     TSGLYVRGGGPDQNLVLLDGVPVYNADHLFGFFSVFNPDAISNVTLIKGGFPAPYGGR
                     LSSVVDIRLKEGNLRKFSGEASIGTVASKFVVEGPIIKDRTSFIISGRRTYIDVLAAP
                     VIGAINRAAGGSSKLRAGYYFYDGNIKLNHKFSDKDRLFVSAYMGRDKAYVRNENDYI
                     YLDTINTSYDDNGLYWGNITTAVRWNHVFNKKLFGNATAVFSDYKFDTYIHYREERGN
                     QTIGNYEYDYFSGITDWGGKLDFNYYPSPKHEIRFGANYLYHTFKPGVSTFRQSADSS
                     AAINLTFGNRPIYANEYYAYVDDNITVTARLKADLGVHYSGFFLKNKWYQSWQPRVSM
                     RYLINENISLKAAFTQMDQYIHLLTNSTVGLPTDLWLPSTDSIHPQRSYQGAVGIAYN
                     FRDIWDFTIEGFYKTMDNLIEYKEGASFFDIGEKWESKLEIGRGWSYGVEFLARKNIG
                     KLTGWIGYTLAWSWRQFDNLNFGEPFPYKYDRRHDVSVVVMYQLNENIDFGATWVYGT
                     GNAITLAVAQYEDLNSGLSNYYFYGDPVEHYNGRNSYRSPAYHRLDLSANFHKKIKIG
                     ERTWSVGLYNAYSRQNPFYLYFGYNNFGQRVLKQVSLFPVIPSVRYSLKF"
     gene            15988..16977
                     /locus_tag="PLK75_06875"
     CDS             15988..16977
                     /locus_tag="PLK75_06875"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOY39007.1"
                     /translation="MKKAILFFGMFLLLFSIACEKIIEIDLEATEPRLVINAIICPDS
                     AVTVCISRSMHILDNHNIVRITNATVKLFDNGIELAQLQHQGNGIYTSPLYPVAGRTY
                     TVIASAPDFEEVTAVCPIPIATGIVQIDTAILKDEYDYPFLNLQVHFNDPESVENYYM
                     LQAYQKYAYEEYDPYGFVVDTLYQNEDTVIVDTTWGIYTTRLAYEPLYLNSSDLIAEE
                     VSTGTNAIVFSDALIDGKPYSFKAYAYVYVYTTDDEITMYLYLKSISKDLYLYYKTLA
                     AHYNASGDPFAEPVFVYSNVENGMGVLGGYSYAIDSIKLNPEDFQGGWYYMEK"
     gene            17059..18591
                     /locus_tag="PLK75_06880"
     CDS             17059..18591
                     /locus_tag="PLK75_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOY39008.1"
                     /translation="MFTPDDVVTLRKRGIQPDEAYNQIEMLRKGTQFIELQAPATEGN
                     GILALANETERFCRIFDAQRNRIAMVRFVPASGAASRMFKRLIDVASKTNLPADDFHG
                     ILADMEFYSVGNTLRNINRFAFARQLAHLLGVKYPIEVNEKNFRQIAAAIIQDRGLNY
                     MNLPKGLLLFHEYEGFCRTAFEEHISEGLQYAVGKCNCLNQHFTVSPEHLNAFTKLKD
                     RVVASYEKSVELQIEFSQQLPSTDTLAVDEDGHPFRNADGSLLFRPGGHGALIENLNT
                     LDAELVFIKNIDNIVPDYLKPETITYKKLIAGVLLEIKDTIFTFLKKLDQNTINQAEI
                     DVMMDYARNVLNIVTAEKRDVYLDSVAFLRKKLNRPIRVCGMVKNTGEPGGGPFWVKH
                     SDGSKSLQIVEKAQINLSISDQRMAFDASTHFNPVDLVCWITDYNGKKFDLLQFRDNN
                     TFFVASKTHNARALSALELPGLWNGAMADWITLFVEVPLITFNPVKEVNDLLRSEHQP
                     VQ"
     gene            18604..>18988
                     /locus_tag="PLK75_06885"
     CDS             18604..>18988
                     /locus_tag="PLK75_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOY39009.1"
                     /translation="MSRKKIPLFVWILTAMLAGIAFGVIATLTDATQFTAFYVKPWGV
                     IFINLLKMVAMPLIFFSLVKGISDLKNISSLSKMGLKTMLLYLCTTLLAVGIGLVLVN
                     AIKPGESFPGDNVRLLETRFEQTISN"
BASE COUNT         5490 a         3940 c         4114 g         5444 t
ORIGIN      
        1 caacagtaat actctcattt tattcgatga tcacgacgac ggcagcattc cctacaccat
       61 tctcaggccc accgaccccg ttgcacaatt tatgggcact ccgcagcttt cgatgcttaa
      121 cggctcggag caggtttatg tgccacgcca gcgttatgga gccggaacga ttgcccgctg
      181 gaatccggga gctaaaatac ttgtatacga cccaactcag gctaatgttc cggtattaaa
      241 ttccgacctg agcaatgccg cagtgcttat ggtgtatggc aggggcttcg atgatgacga
      301 caggggatgg gtgatgtatg aagctgcaca cactttgctg aaaggaacta ccagcgatgg
      361 tccggcaatc agggcgttct tcaatttttc gttttttgcg cagattgaac gggcaccgca
      421 gataccatcg aatgctattg acggtatttc acccaacggt tattatcaga gcggtgagca
      481 gatacatttg agtgttgatg cggttgcagg gctttcaact acttcgattt attcctatac
      541 atggatggtg gctgttgatt atggtgaata taccacaaca gggtttgatc cagcttacag
      601 tacaacatcg aattcaacca cgtacacagt tccttatacc cccaccattg ctgaaggcct
      661 ggtttcgccg gcgattatca ttgtaattgt atccgataac tgtggacgta ccaataccat
      721 ttcggttcct gttaacttta ttcccaatcc actgcctcct attcctgttg atgatgagta
      781 cactcttccg gggagctgtg atccggggct ttctctatcg ttcaatgtta tggaaaatga
      841 tgcggatgca caggaatcaa ccctcagttt ttacagcttg aatcaggcaa cagcaaccac
      901 cactcctgca ggcgatgtaa cctgggttga caatggtggc ggaaacatta ccatttaccc
      961 tgatgtgaac tacaacggtg tggcgaccat tgaatacacc attatcaatg agttcgattc
     1021 cgttgcaagt gccactatta cggtaaccat cggttctatt gatgcagatg gttgtttatc
     1081 gaaccagttc tggggagtga atgagtataa gtttattact aatgatcagt tggtctccag
     1141 cactaccccc cccggtgcaa caaccaccgg aacacagctc gacgattatg agtcgggata
     1201 ccaaaatgga acgtatctgg actttcagaa agcctacacg aacaacatca tactggcgat
     1261 cggatctgaa cagtcgcttg ctgagcgcga cattgtgatg ttgaacacca gaacacaaaa
     1321 tggcagtggc acaatttcga tagagatagg gcaatcgtct tcaggcccat ggaccgatgc
     1381 ccagactttt accattaatg atactgaaag cattaaaaac tacacgattc ctacaggtgt
     1441 taccgacatt acccatataa gaatatcggc aggcaacatt accacaccag ccaactcggc
     1501 gaataaagtg tatctgagtg cagtaaggtt ttacgattac gattgtgtgt cgcgtgcgcc
     1561 cagagccact aacgacaata tcacaatact cgaagacctt cctacggtaa tatcggtaac
     1621 cgccaacgat tacgacccca acggattgaa ttttgtttta aaaaacatca ttacttctcc
     1681 atcgcatgga catgtgagta tcaacaccga tggtactata acctacgtga acaccaccga
     1741 ctattcggga acggatagtt ttacgtatca ggtatgcaac gactcgggat attgctctac
     1801 tgccactgtt actattacta ttgttgacga tgggtgctcc tcggggagtt acaaacccac
     1861 atcgggcagt ggtacgatta ccaagatttt tcagtatcaa tactcgggga ccaatgccgc
     1921 taccgccaat ttaacttcgg ataactttcc tgacagttat atcaagcagg atagacccaa
     1981 tgaaaacaag gggggctcca gtgccaaatt agaggtagga aaaaaatatt caggaacaaa
     2041 agcaaggcga gccttgttct atttcaacgt ctcggaaata ccaacggatg ccattataca
     2101 aactgccaca ttttcggcat tgcgcacagc cggcgatggt gtgtcgcagg caattaacct
     2161 gcatcaaatt acacaggact ggtcagaaag cgaggtaaca tggaaccttg cctactcgga
     2221 gcacaactgg acatcagccg gaggaacttt cggttcggtc ttatcatcca caactgttct
     2281 ggccaacgga agatactatt gggatgtttt aaactctgtc caagcttggg tttcgggttc
     2341 ttcttccaac tatggggtgt tgcttaaaac aggagaaacc attgataaaa agcatgtgtt
     2401 tgcatcgaga gaagatggga cggtttcaaa acgtccaagt cttacagtta cgtatgtagt
     2461 tccggcctcg tgcgaagcca ttccgaaccg caatccgctg gccaatcccg attattcgga
     2521 gacctattca tctgctgccg ataccatagc agtgcttgac aacgatactg accccgatgg
     2581 caacagcatc agtatctccg gtattggcac agttactcac ggcagtgctg taatcagtgg
     2641 caacaacatt atatttacac ctacctacgg ttacaatgga atcggtacgg tggtttatac
     2701 cattaaagac aatggctcgg gtgaacttac cgatcaatcg acaatatatg ttcaaataaa
     2761 gaatgcaccg acggtagcaa tttacgatgt cgattcggtt gattcgaacg atgagttggt
     2821 aatttatgta atagataatg atgaagatcc tgaggggcaa acctccacaa taagccaaat
     2881 tgtaacccag ccaagcaacg gaactgtcga aatatcggga aatacgctag tttttaatcc
     2941 ctatccaaac tttaccggcc gcgattcgtt ggtttatgaa gtttgtgaaa cctatgtaga
     3001 agaatgcgat gccacaaccc tgtgcgacac cgcaatagtt tacataacca ttaataaccg
     3061 gccgcctgaa attgttcctc cactaatcga cacagttgtt ccctgtttct gcagcgtaat
     3121 caacctgcat aattatgtaa gtgatccgga agaaggctcg ttgaccttta caatagtatc
     3181 aggtcccact cctcccggag gaggcaaagc agggtcgggc acactcgaaa acaatggcga
     3241 tggaacggta agttatactc cacctattcc gctcgacgct cttgagtaca tcacctttac
     3301 ctttgccgta agcgacgatg gtaatccaac caagacctcc gatacagtag aagttacttt
     3361 caggatagta cccaaagaca atacagcccc tgtagcagcc gacgacgatt ggctcgatgc
     3421 agtgatgaat actgatgcca acgtaagcat tggtgacaac gattacgatc ccgatgggaa
     3481 ccctttgagt ttaccattac tgattacgca accactgaat ggaacagcag agattctgcc
     3541 aaacggatta ctgaggtaca cccccgatta tcaattctat ggaaccgatt cgttgtatta
     3601 tgagattcac gacactatca ctccaagttc acagacttgc cagaacgaaa aaggcacttc
     3661 agatacagcc aaggtaagga ttaccataaa ctacgatccc gatcagttcc tgccggtttc
     3721 attggtgttg tacgaagcaa agtgcaataa cggagtaacc gaaatcgact gggcaacagt
     3781 atcggaaagc aacaacgact actttgttat cgaaaggtcg aaagatcttg tcagtttcga
     3841 agaaatagca cgtgtgaacg gtcagggcaa cagcaacgat tataagtttt atcgatactt
     3901 cgaccgcaat gcagcgagtg gcaccaacta ctaccggctg aagcaagtcg attacaacgg
     3961 caggcttacc acctacacca cggtaaattc atcgtgtagc agcagtgtta gcggggagtt
     4021 gatggccttg gtttacccca atccgttcaa cacgcaactg aatgtatcaa tcgagaatgt
     4081 tgtcgaagga gaggttgtga tagaactgtt tgatgttgcc ggccggaagt tgttcggttt
     4141 gaatgaatac aattattctg aaatttattt ccgtgaattg aatatcgata atctggtaac
     4201 tgggatgtat tatttgaaaa tacacatcaa tggtcagatt attactaaaa agcttgagaa
     4261 gaaataacaa gtattgaaca aagaaaaagg cgggatgacc gccttttttt tatgtaaaaa
     4321 tgagagtagc cgcgcgtcat tgcgaatatg aggcacgaat atgaagcaat ctgcatttac
     4381 gggcggtaga ttatacgact ccgagtgggt ggatagcttc actgtgttcg ctatgacgtt
     4441 atggagcccg tcattgcgag ggttataaac ccgaggcaat ctgtattcag acgggcgatg
     4501 ataaattcag tgctgtattg gtagattgct tcgacttcgc tcagggtgac agaacgattt
     4561 agcaaaaaat cctttattta ttaaaaattc cgcctctctg gtatatttcc atttgcactt
     4621 tcgaaaacgg attggcttgc accgaagtcg atttggtaag attttcgatg agtccggttt
     4681 caaagttcac ccggatggta tcgccttcgg taagattcag cgcttcgata tttttatacg
     4741 ttagaatggg catggctgca ttaaccgcgt tacgttcgta gatagcacca aacgattctg
     4801 caacgataca tgatatgcca agcgatgcaa agcagtcaac cgcttgctga cgcgatgatc
     4861 cggaaccaaa gttcttttgc gtaacaacaa tgtccccagt ttttgctttt ttagcaaagt
     4921 cctcatatcc cttcaggtta tcgaaagtat attgtcccat ctggcttata tcggtaatag
     4981 tgaggtagcg gttatgataa atcatatcgg tatcgatatt gtcttcgtga atgaaccacg
     5041 cttttccttc aacaattttt ggtttgggtc cggtggtctt ttttgcatca tttccagttt
     5101 tttttgcgct tatgtctggt ttagcaaact cagccggttt tgccggaata ttgtcgggag
     5161 tagtaataaa tcctgctact gctgaagcag ctacaatgtc gggggaagta aggaatactt
     5221 cgcctttgcc ctgttttccg gggaagttcc ggtttccggt actgacggta atttctgcag
     5281 ggccgttttg ccctacctga cctgctgcac aaccggcaca accggcattg gaaaccatgg
     5341 ctcccgcatc cttgaaaatt tgaatcagac cttcgtcgag agcctggttc caaatggagt
     5401 cggtggcggg aactactttg agcaccacgc ccggagctac tttccgacct ttcagtatgg
     5461 cagcagcttt tcgcaggtct tccatacgtc cgttggtgca gctgccgata aaggcggagt
     5521 cgattttttt accgtattga ctgcttacat caacactgtc gtggggtttc ccgggtttcg
     5581 agaccatagg gacaaatttc tcgatgtcga tagtttcatc gaatgcatat acagcatcgc
     5641 tatctgccgt tatcttttcg attttctttc cggtggcttt ttcgctatag cggattattt
     5701 cgtcggaggg cgggatgatg atgatgatgg cacccatttc agtagccatc gaggagatgg
     5761 taattctttc gtcgagagtg agtttgtcaa cggcttcacc atacaattct acggaatagc
     5821 ccagcagtgt attggcacca tagcgcgcca gtaaattgag aacgatatct tttgcagtta
     5881 catttgcagg acgcttacct ttcaaagtaa tttttgctga ttccggaacc ttgaaccaaa
     5941 ccgatccgtt tgcccaggcg gcagcaatat cctgatcgcc catgccctgt ccgaacgaac
     6001 ctatagcgcc cattatattt gcatgactgt cggtcgatac aaaagtgctt cccggaaacg
     6061 caagtccttt ttctatggca agatgtgtcc cgatgccacg gtcgatatca tatactgcta
     6121 ttccgttggc cctagcaaat aaacggcatt tatgctgatt ttcagcatat ttctggtcgg
     6181 agcccgtggg gttgcaatcg aaagtgaaga aagttcttga ggcatcctcc acctgcagtc
     6241 cttcgtcaac aaggttctta accacgttgg ctcctccgaa atctcgggca acacgggcat
     6301 caataaaaat atcgacgata tcattggcct ttacattttt ttcgttgctg tggttagcaa
     6361 gtattttttc taaaattgtc attcccattg tattgtattt atgatttcat gcaaatttat
     6421 gcaaatcagt cagtttaaaa acaatagaaa catttttaaa aacattattt cattggataa
     6481 aacaccaaaa gttgcttact taagcatata gtaatagata aacaaaatcg tatattgatt
     6541 taaatttatt ttgaaaaacc cgttattaaa ttgttcgtca acaaaagtag catagcgtct
     6601 atcgcttttc gatttttttg atcaatacat ttttaccaga ggtaattcta acaaaataca
     6661 ttgcgggagt tagacctgac aggctaagat tcttattgta ggtatcagaa acctgatttt
     6721 ctgcgatttc ggttattttt cttccatata aatcgaacac ttcgatgcgt agtttttcgt
     6781 tgcaaggcag gttcgataga gcgataatta tataatcgct gaacgggttt ggaaaaatca
     6841 gcacttcggg aggtagaagc attgagaaaa gagaacccgg gtcgttttct tggaaattat
     6901 ctttttcgcc cccgagggtt atcgtggcaa tgttgctgta cacataatcg caccccgctc
     6961 cattggaact tattttaacg cggtaacttc gcgtgtatgg tgcctgggta acaaactcag
     7021 gggaattggc tcccggaata tcaatccaaa ttccgttcat aaagtattgc cattgatata
     7081 gtaaccccat ccctccatct gcaacaactg caataggaaa ctcttcgccc ggggcaagga
     7141 tggtgccccc ttcaggttgt gttgtaatgt atggatcggg atgtacggtt actgttacag
     7201 gagttgaata cgctgtgttg cagccgttgc cgtgggttga taatgccagc ctgtattgtg
     7261 tggtcacgga aggatagaca gtaattttat aatttgtttc accatccatg tcgcgccaaa
     7321 acccgtcatc gtaatactgc cactgataag aacgaactcc acagccgccg gtaacagcag
     7381 tagcgatgag aaacgcagat tcacctgcgc agaactcgta gttaccatta attaaaattt
     7441 gcgggtcgtt gtaaatagta agtaaggctg aattcgaaat gacggtattg cagcccggtc
     7501 cttcagcgct tacaacgcaa cgataatagc gggttaatgt ggcattcgaa atggttagcg
     7561 aattacttgt ttcctctgcc aaattagtcc agttactttc ttcagccgat tcctgccatt
     7621 gataattcaa cccagtaatg cctcctgttg ctgttatggt cattgtatga gtaccacctg
     7681 tgcagatatt acggtttaat ggttgcaaag tgattgcagg gtcgggatag acggtaacgg
     7741 taaccggatc gcttgtaaca gtaactgtgc cgaggccgtt attgccaatg gctttcaccc
     7801 tgtattgccg tgtgtatggg gcatttgtgt ttagggttga ttgcgtaaaa cccggggcaa
     7861 ttttccaaac accattaata aaataatacc attgataagt aaatgtgtag cctgtgccaa
     7921 cagctgttgc agttaaaaga aaagagcctc cctgacatac ttcaccatct tcagggctaa
     7981 ccgataccga gggcaatacc atagtaacga tttcgtcgta gccatagcaa acaatattat
     8041 ctgttataaa caattcgtaa tttccaaaat tctgaattac agtattattt ataatagggt
     8101 tttgctcttc ggatgtgtag ttgttcggtc ccgaccagtg gtagcaacaa tccatcgaag
     8161 aatagccggt aaattcaagg gtatttccca aatgtatcgg tgaattagag cttgcagaaa
     8221 ccactgtgtt gaatgaaaga aaattatttg tattaaactg agtacaaata ctgtagttca
     8281 tataggtgtg caatgaatta tagatgtcgg taacccctct ccatccgccc aaactgtaat
     8341 aattggttcc tgtccagagt aaattatcca attcgctggc attcgcttct atataaactt
     8401 tggcaataaa gttctctttt acatgcataa gaatgtaatc atcgacaaag cccagcggta
     8461 tgggagtagt tccgtttgat ttggttacaa taacccgcat tctaacatgt acagattttg
     8521 atatccactg gcctgatgcg tttaagcaat aatatgactg cacacctgtg aaacacattt
     8581 tcccattgac aagattcgtc tcagtattta tcgggtcgaa tacaagggtt aaaaaattgc
     8641 atccataatt tggtggctga gtattattca aatttaatac cgcttgattc ttatacagcc
     8701 cccagttgat acattcgggc caattggcat tattgtaaat cacctgcaat cccactgcag
     8761 ttcccagtgc attacttccg ctaccgggta taattgtaat tgatgtgcca cccgggtttg
     8821 cttcgggttc aacttctgcc gataagttac cgtaatagaa actttgggta tcgaaagtag
     8881 tacaaatgct acgggatggg tttgttgcaa gcgcattgta aacatcaata acaccatacc
     8941 agccaccata attgtagtaa tttgtcccta cccaaactac attatccaga ttgctggtca
     9001 gcacttccat gtaaacacgt acaacaaaac tggcactggg aactcctgtt ataattccag
     9061 attgcagact cagcggtacc ggtgttgaaa gattggtata ggttaccaca acgcgcattc
     9121 taacattgca ggttttatta atccattgtt gcgaagtgtt caaacaataa taagattgct
     9181 gacctgtaaa gcaaattttc ccctgactca gattggtctc tgcgttactg gcatcgtagg
     9241 tgaggtttaa aaagctacaa ttctgatttg gcggaaggtt gttgttaaga tttaaaacgc
     9301 tctgcacttc gtcgagtccc cagtaagtgt tctgcggcca gccggtgaaa ttgtaattta
     9361 ttctgaatcc ccccggcctt ccaattgaat tgcccattgg attgggaacg gttgtacatg
     9421 taactccttc tggtatctgc cctaaataaa caaatgctga ttttgggtga acaacaggag
     9481 attccggatt ttgcgcctgt actgctccaa agccttgaaa taaaatagta gtgctgctta
     9541 acaaaaagca ggtggcagaa ctaatccaga gaacaattaa acaaattact cgtatcatgg
     9601 ctacttgaca attagtttac tatattgggt aacagtttcg ctttttattt caatcacata
     9661 taagcctgat gacaactccc ctgtaaacat ttcccccagg tttccgtcaa aacttttttc
     9721 ttgtaagagt tttcctgaaa agttataaat acgggctgta aagtgatggt attcaacggg
     9781 ggccttaatt ctaacaaaat tactgcatgg atttggataa agagagcata tgctataaca
     9841 cgacacttgc aaaaccggaa tgaagtcgcc gtcaaatccc agtttgtcaa ttccaagggt
     9901 tgatgcggct gggttatttt tgctggttga acgtatggtg atttttgcga aatcaggcgt
     9961 ggtattttca tcggtgtaat gaattggtac gataaacttt gtccaatcgt aaacatagta
    10021 atcgtattgt agaaagatgt tgaaagaacc tgtgcctaca gtatcatcac catggaatag
    10081 cattaccgaa acttttgcgg tatcctgagt atcggagtag taggtgtagt acccttccag
    10141 tgttgtatgt ctcactgaca cgggaaataa atccgtataa atttctcctt caatgttttc
    10201 gttacgtcct tcggaaataa cttcctgaat gttgattcct gtaggactga gatgcccgat
    10261 agtgttttta ctgaagggga ggggaggcat ttcttcaacc ggaagatagc tgtattcata
    10321 aaaccagccg gtaggtaagg tgttatccca aatttcgaag tcccagttgg ggattggatt
    10381 accggtgtag aagcttttta attccccgaa atagttctga gagtttccgt cggtgagttt
    10441 aaccctgaag aaatactttg tatttatgct cagctcactc aggtcataag tgatattgct
    10501 tattccactg ccctgaattt cacccatatt gatcagatgt tccgtatttt catcggttcc
    10561 ataacagaaa aatgctgtac aagattcatt aaccgactgt acctgagcat tcaaggtagc
    10621 tttatatttg ccgacattgg tagcattatt tgtggccacg agtggagaac catcgtaata
    10681 tttaataaaa tgcccgaaat tgccacccac agcacccaga tgtgtattgg taaagcacac
    10741 tgaataaaag tcaaggtatg taatttcgga aaaatcgatg ctttgccagg tttcacctcc
    10801 attggaagac ttcaggatag taccgtagtc gcctgctgca actaagttat tttcatcaat
    10861 taccgatata cagttcaggc gtggaccagc ttcttccaaa atacacagcc aggtaacgcc
    10921 tccatcgatg gttttcaaca ccactctggt ggagtcggaa ccccgggtgc ccccggtaca
    10981 gaaaccaata ttgccataaa aaaacaggct tgtgaaatca acagtcccgt taaggctgct
    11041 tatattgaca tctgcccaat tggttttggt ttcatcgtta tatttcaaga aattgttggt
    11101 accacaaatg taaacggttt ggtcaggtga tactgccatt gagattaatt cgttgttacc
    11161 ccattctccg gcaacattcc agctattccc cagatcggaa gaattgagta cgaatgcaga
    11221 atttccgtcc aaaccgccag ccgcccaaat acctgataca tcagaataac aaagtttgtt
    11281 gatatcggca tggtttaacg aaatattgag tgcaacgggg aaccaaagct tacctgaatt
    11341 ttcggttttg tacaaaactt cgttcattcc ccctgcatat ccgatatgtt cattgataaa
    11401 tacagcacta ttcagcattg gattcatcgg atggtctccg aaataatccc attcagcacc
    11461 tccgttcgaa ctgtaggtcc aatacagtat tgaatcattc gaaggattgc caccaacaat
    11521 ggaaacctga aatggactcc aaaaatgaat atcgtttata caacgatacc tcggaacata
    11581 attaaccgac cagttctcgg gagtttgagc cgaaacaggg ttacataaaa atgataaaaa
    11641 cgcaaacaaa accaagtaaa gctttttcat atagtcttcc cataaaatgt tcaacagaat
    11701 aataaggcca atcgattata caaatataat aaaaataata cttaataatg aaaatatata
    11761 ctaataattt tactgtttat aatttgtaat gaaaaaacta tttattcatt gcgaaaagtt
    11821 attcagccaa tggctgaata atttgcaaat cttattttta aaatatccac acctggttgt
    11881 aatatgcgga acaattgttt gtgttggtaa aaaaagtttt ctaaggatta ggtatcaaat
    11941 aaaacataaa tcgaatatcc gaaatatatg tgttaattcc ggcgatattg aatcaaattt
    12001 ataaatttgc attttaatga cggaaccaat ggaaaaccat ccttcacttt cgaaacatgt
    12061 tgttgaattt cttctggtag caaaagaata tgttagtttt tccgaaacac cgcaacattt
    12121 ccaacagtca aattacattg atacctgctt aaaacttttc tccatgcttt atctgaaagc
    12181 cctgttttta ccagatccag atgaaaagga aaaacccgat gaatacgggc tagagtttgt
    12241 gaatgcaatg caatacgagc ggataaaggc gaactttgag caacagttgg gcgaagctga
    12301 tgcgattgtt ccatttaggg atgcgggcat gataaatact gacgattatg tgaatacaag
    12361 tatttcggag cttttggcag atgtatatca ggatgtagcc aattttgtgg aggcattccg
    12421 cataggtgag ccacaactca tggatgaagc ccgggtaaaa tgcagcctga acttcaagtt
    12481 gttctggggt cccaggctga ttgtggttct tgatcaactt caccggttta agtatataga
    12541 aaacaataac aattgaaagt atgttgtttg aatccagcat tagccccgaa gcggttcagg
    12601 cactacccga gctcaagttt gaaggagata taatagttgt tgatacactt caacaactga
    12661 agaaacttgt ccccgaactc attctcgaaa atacctgggg ctttgatacg gaaacaaaac
    12721 ccaattttct gaaagggaat ccgaacccaa atacagtgtc gctgcttcag ttgagcggtg
    12781 ctggtaaaac ctaccttttc cggttaaaaa aaattggtct tcccgatata atcaagcata
    12841 tccttagttc gccaaagatt gtaaaagcag gagttgctgt aagggatgat atcaatgctt
    12901 taaaaaaaat aaaaaccttt agcccagagg gttttaccga gcttcagacg gaagcacaca
    12961 agttcggtat taccgatata tcgttgcgta atttggctgc cattgtttta ggtatgcgaa
    13021 taagcaaagc ccagcaattg agcaactggg aaaatgaaca actcagcgaa aagcagatat
    13081 attatgctgc tatcgatgcc tgggtgtgcc ggcaaatatt tttaaaattt caagcaagca
    13141 ttcatgatag agaaaacagt taattacagg gttatttacg gtgatactga tgcaatgggc
    13201 cttgtgtatt atgccaacta tctccgtttc tacgaaattg gccgaagcga atatatgcga
    13261 agccttggct ttccgtatat gaatctttcg gcaagcggta ttgagtgtcc tgcagttgag
    13321 gtcagaatga aatatcacag cagcgctcac ttcgacgatt tgctcaccat tacaacaagc
    13381 ctgaaggaaa ttccaaaatc aaccatggtg tttgttcaga aagttttcag agaaaatcaa
    13441 ttgcttaatg aagccgaaat acagctttgc tttttgaata tgctaacgca taaaccggta
    13501 cgctgtccgg gtgtgcttcg tgattatttt aaaaatatgg gatattaaca caaaaactgc
    13561 ataataatat tataaagaaa cagttatatc actttgaccc tgtaataatg aaaataaaat
    13621 tccttctgtt cctgattctg gcttttgttt caatgcttgg ctactcgcag aaatatacaa
    13681 tttccggtta tgttgaagat aaaagctcgg gtgaaaaact ctacagtgcc aatgtgtacg
    13741 atatgcgaac aaaagcgggt gcaattacaa acgaatatgg ttttttcagt cttacactga
    13801 aaaaagattc tgtagatctg gtagtctcgt ttgtgggttt tacctcatat agcttcaggt
    13861 tttatctgga tagggatatt cagctgaaca ttaagcttga tcctgttatt cagctggcag
    13921 aggtggtaat taccgataaa aaagccgatc aggtggttaa agatgttcag atgagtgtta
    13981 tagaaatgcc ggtgaaccag ataaaaaccc ttccggtatt tatgggtgag gctgatgtga
    14041 tgaaaaccct ccagctcatg ccgggagttc agtcgggtag cgagggtacg agcggtttgt
    14101 atgtgagggg aggcggcccc gatcagaacc tggtattgct cgatggggtt cctgtatata
    14161 atgcagatca cctgttcgga ttcttttcgg tgtttaatcc ggatgccatt tcgaatgtaa
    14221 cactgattaa gggcggcttc ccagctccct atggcggaag gctttcctcg gttgtagata
    14281 tacgtcttaa ggagggaaat ctgagaaaat tctctggaga agcctccatc ggaactgtag
    14341 catcgaaatt cgttgtcgaa ggtccgatta ttaaagacag aacttcgttt attataagtg
    14401 gccggcgcac ctatatcgat gtgctggcag caccggttat tggtgccatc aacagagcag
    14461 ctggtggatc ctcaaaactg agagcaggat attatttcta tgacggaaac attaagctca
    14521 atcataaatt ttcagataaa gacaggttgt ttgtgagtgc ttacatgggt cgcgataaag
    14581 cctatgtacg caacgagaac gactatattt atctcgatac aataaatact tcttacgacg
    14641 ataatggttt gtactgggga aacattacca ctgccgtacg ttggaaccat gttttcaata
    14701 aaaaattatt cggcaatgca actgcggttt tttctgatta taagttcgat acctatatac
    14761 attacaggga agagcgtgga aaccagacaa taggaaacta tgagtacgat tatttctcag
    14821 gcattaccga ctggggcggg aaactcgatt ttaattatta cccctcaccc aaacatgaaa
    14881 tacgtttcgg tgccaactat ctctaccaca cattcaaacc aggggtatcg accttcaggc
    14941 aaagtgccga ttcttctgca gcaatcaatc ttacattcgg caacagaccc atttatgcaa
    15001 atgagtatta cgcttacgtt gacgataata ttacggttac agcgcgactg aaagctgacc
    15061 ttggagtgca ttattccggg tttttcctga agaataagtg gtatcagtcg tggcaaccac
    15121 gcgtttcgat gcggtatctg attaatgaaa atatttcgct gaaagcagct tttacacaaa
    15181 tggatcagta tattcacctg cttacaaaca gtactgttgg cttacctacc gatttgtggt
    15241 taccttctac cgacagtatt catccacaga ggagttatca gggagccgta ggtattgcct
    15301 ataatttcag agatatctgg gattttacca ttgaaggttt ctacaaaact atggataacc
    15361 tgattgaata taaagaagga gccagttttt tcgatattgg tgaaaaatgg gagtcaaagt
    15421 tggaaatcgg cagggggtgg tcgtatggtg tggaatttct ggcaaggaaa aatattggaa
    15481 aactcacagg ctggattggt tacaccctcg catggtcgtg gaggcagttc gataatctga
    15541 attttggaga accctttccg tataaatacg acagacgtca tgatgtttcg gttgtggtga
    15601 tgtatcagtt gaacgaaaac atcgactttg gagcaacctg ggtttatggc accggtaacg
    15661 ccataacgct ggcagttgcc caatacgaag accttaattc cggcctttcg aattactatt
    15721 tctacgggga cccagtggag cattacaacg gaagaaactc ataccggtcg cctgcctatc
    15781 accgcctcga cctgagtgca aattttcata aaaagattaa aatcggggag cgtacatgga
    15841 gtgtaggtct gtacaacgca tacagccgtc agaatccgtt ttatttgtat tttggttata
    15901 acaactttgg gcagcgggta ttgaaacagg taagcctatt tcctgtgata ccttccgttc
    15961 ggtattcact taaattctaa ctgcgctatg aaaaaagcaa tattattttt cggtatgttt
    16021 ttgctgctgt tttcgatagc atgcgaaaaa attattgaaa tagatcttga agcaacagaa
    16081 cccaggctgg ttatcaatgc tattatttgc cctgattccg cagttaccgt gtgtatctcc
    16141 agaagcatgc atatcctcga taatcataat atcgttagaa ttaccaatgc aacagtgaag
    16201 cttttcgata atggaataga gctggcccag ctccagcatc agggcaatgg tatatacact
    16261 tcgccattgt atcctgtggc aggtcgcaca tatacggtaa ttgcatcggc tcccgatttc
    16321 gaggaagtaa ctgctgtttg ccccattccg attgccacag ggattgtcca gattgataca
    16381 gccattctta aagatgaata cgattatcct tttctgaacc tgcaggtaca cttcaacgac
    16441 cctgaatctg tggagaatta ttacatgttg caggcatatc aaaaatacgc gtatgaggaa
    16501 tacgatcctt acggttttgt tgtagatacg ctgtatcaga acgaagatac ggtaattgtt
    16561 gacacaacat ggggaatcta caccacgcgc cttgcatatg agcctttata cctgaattcc
    16621 tccgatctga tcgcagagga agtgagcaca ggtacaaacg caattgtatt ctccgatgct
    16681 ctcatcgatg gaaaacccta tagttttaag gcctatgctt acgtgtacgt ttatacaaca
    16741 gacgatgaga ttaccatgta tctttacctg aagagcattt ccaaagacct ttatttgtat
    16801 tataaaaccc ttgcagcaca ttataacgct tcgggtgacc catttgccga gcctgtgttt
    16861 gtgtattcca atgtcgaaaa tggcatgggt gtgctgggtg gttacagtta tgccatcgat
    16921 tccataaagc ttaacccgga agattttcag gggggctggt attatatgga aaaataataa
    16981 attgaccgct tccttcttta atgtttagtg ttttaaaagc ggattagaat tactacattt
    17041 gtttcaaaca atcgaaacat gtttactcct gatgatgtag tcacactgag aaagcgcgga
    17101 attcaacctg atgaagccta taatcaaata gaaatgctgc ggaaagggac acagtttatc
    17161 gaactgcaag ctcctgcaac cgaaggcaat ggaattttag cacttgctaa tgaaactgaa
    17221 agattctgcc ggatatttga tgcgcaaaga aacagaatag ccatggtacg ttttgttcct
    17281 gcttcgggtg ctgcaagcag aatgtttaaa cgtttaattg atgtagcttc gaaaacaaac
    17341 ctgccggctg atgattttca tggaatattg gcagacatgg agttttattc ggtggggaac
    17401 acactgcgca atattaatcg ttttgccttt gccagacaat tggctcattt gttgggggtg
    17461 aaatacccca ttgaagtaaa cgaaaagaac ttcaggcaga ttgctgcagc aattattcag
    17521 gataggggac tgaattacat gaatttaccc aaaggtctgc ttttatttca tgaatatgaa
    17581 ggtttctgca ggacggcttt tgaggagcat atttccgaag ggcttcaata tgcagttgga
    17641 aagtgtaatt gtctcaatca gcatttcacg gtttcacctg aacatttgaa tgcttttaca
    17701 aagcttaaag acagggttgt tgcaagctat gaaaagtctg ttgaactaca aattgaattc
    17761 tcgcaacagt tgccatccac cgatacacta gcagttgatg aggatggtca tccatttcgt
    17821 aatgcagatg gtagccttct attccgcccc ggtgggcatg gtgcattaat cgaaaacctg
    17881 aatacccttg acgcagagct tgtttttatt aaaaatatcg acaatatagt tccggattat
    17941 ctgaaacccg aaacgattac gtacaaaaag ttaattgccg gcgttctttt ggaaataaaa
    18001 gacacaatat ttacattcct gaaaaaactg gatcaaaaca caatcaatca ggcagagatc
    18061 gatgtaatga tggattatgc caggaatgtt ttaaacattg ttacagcgga aaagagagat
    18121 gtttatctgg attctgtggc ttttctacga aaaaaactta atcgacccat cagggtgtgc
    18181 ggaatggtta aaaataccgg tgagccgggt ggaggacctt tctgggttaa gcattctgat
    18241 ggaagcaaaa gtctgcagat agttgaaaaa gcccagatca atctgagtat atccgatcaa
    18301 agaatggcat ttgacgcatc tacccatttt aatccggtgg atttagtctg ctggattacc
    18361 gattataacg gaaaaaaatt cgacctgctc caatttcgcg ataacaacac tttttttgtt
    18421 gcatcgaaaa cccataatgc acgggcgtta tcggctcttg agctgccggg cctatggaat
    18481 ggggcaatgg ccgactggat tacgctattt gttgaagtgc ctttaattac ttttaaccct
    18541 gtgaaagagg taaacgattt actgcgcagt gaacatcagc ctgtgcaata attattttaa
    18601 agcatgagcc gaaaaaaaat accccttttt gtttggatac ttacagcaat gcttgccgga
    18661 attgcttttg gcgttattgc tacccttacc gatgcaactc agtttacagc attttatgtt
    18721 aaaccatggg gagtgatttt tattaacctg ttgaaaatgg ttgccatgcc tctcattttt
    18781 ttctctctgg taaaaggaat atccgattta aagaatataa gcagtttgtc gaaaatggga
    18841 ctgaagacaa tgttgcttta tttgtgcact acattgttag cagttggtat tggtcttgta
    18901 cttgtaaatg caatcaagcc aggagagtcg ttccccggag acaatgttcg attgcttgaa
    18961 acgcgttttg aacagactat aagcaata
//
LOCUS       DAOOQP010000051        18881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3561_length_18881_cov_5.528577, whole genome shotgun sequence.
ACCESSION   DAOOQP010000051 DAOOQP010000000
VERSION     DAOOQP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3561_length_18881_cov_5.528577"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2718
                     /locus_tag="PLK75_06890"
     CDS             <1..2718
                     /locus_tag="PLK75_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39010.1"
                     /translation="QNNAMSASSIYSIGVSQGDIRNVQRLIFPNQTNIPNGFSYYLTA
                     RQAPTEFQLSPEGVLTGIPTQEYVSEDFYFKVTDNHNLIDRKTLPFVTDGLIMQFIPH
                     TPDDDIPEYNETVDIEITATNPMTSAVTGISVTMTSADSYIIITDANATLPNINPGAN
                     TNLLNALQFQINANTPDQHTALFNFSVSSDQDTWNYAKTFTVNSPKIQPGDVTITDGN
                     DNVLGAGENATVTVEIFNSGHAPASHINISLSTSDPYLTLNSDPADIANLAASGMAES
                     SFSVSAASIIPETHIAVITMQVVADNGYTCSESFEVTIHTPVITAGSLTVNDGSNFCL
                     DAGETTEINIPLNNVGQISAHNVVAQLSTYDEFVTINEATYSTPSINFGESAISSFNI
                     SVSSDAPLAHYVVFNLHITAADGFDVTIQVATIVGLMIENFESGDLTLFDWITTAPAP
                     WFITTEEPYEGSYCLRSGDINDSQSTTLSIENTVVVEGDISFYYKVSSENYYDFLEFL
                     IDNVVIASWSGTHPWSQYNYTVATGTHTFAWRYRKDGSISNNSDCAWIDYIVFPAVNG
                     LPPVFALEPASVEKWMYSDETDTDTILISNAGGGIIDYTVGVDFIDVKSTKNITGSTL
                     TCNTNSFMAGGTYDFVFTAHNASNDSEWLKSITIDFPAGFIVNSYTDFIGGSGGNLIS
                     TDPVGDGASVTWTTANQWGVIYDGESAVVTVNVTISSGFGNMLADIDYTLTGDIYGAE
                     PHTLEGVILMANQGNFWLTVIPFSGQLLSQSETELYLNFNTEGMDNGYYYANLSITHN
                     NEVSVVPITLHIVTVDVPESVQENVQIYPNPFSNTVYINTANLTEPIHRIEITDVQGR
                     LLYVCSNREISANGNCIWLNTQGFISSTGIYFVKIVMEQKTEIYKLIKE"
     gene            complement(2762..3325)
                     /gene="efp"
                     /locus_tag="PLK75_06895"
     CDS             complement(2762..3325)
                     /gene="efp"
                     /locus_tag="PLK75_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOY39011.1"
                     /translation="MATTADIKRGLTIEFNGKLYQIVYFQHVKPGKGGAFVRTKLKSL
                     ENGRVIDNTFNSGVKINTVRIERRPYQYLYKDDMGFHFMHNETYEQIALSQELIENAD
                     LLKEGQNVEMMFHAENDQVLTCELLPTVEMEIVYTEPGIKGDTATNTLKQATIETGAT
                     IMVPLFVNVGDKIKIDTSTREYVERVK"
     gene            3493..5934
                     /locus_tag="PLK75_06900"
     CDS             3493..5934
                     /locus_tag="PLK75_06900"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39012.1"
                     /translation="MKIRFTILIIFLLFLGYSLSASIIYHNVNPDEVITPLAAEYFID
                     MNSDAGNDFSIQNNVDAGNNIFEILFGCIGAGADVVGTDIGGVFCVSVLTNGTNIGAG
                     SGFSGQAPISYPSLRTASYTSWLGQTNKFIGVKFMIGANTYYGWIRITVSGTNVVTVV
                     DWAYNDIADGTIAAGDMGGGGTETLEANFLASSYNINIGECISFQDNSMGNPTFWSWT
                     FDGAETPTSNLQNPEGICYYTPGVYDVTLNIQNATNTDTYICVGCITVIDQTIVPIAN
                     FIADQLVIPAGSSVQFTSTSQNGPFTSFAWAFEGGLPFSSTDETPMLISYSTPGVYDV
                     QLTVVNTQGNEDTELKQDYIKVVPLSTELPTVNFIADRTLIAPGESVNFHDLSHGHPY
                     QWEWNFQGGLPVISYDPNPEGIVFPNPGTYNVQLIAINSLGSDTLIRNAYITVTDNPT
                     CTAPPVPNFRASNRLIVASNKVYFEDFTTNNPFTWNWYFEGGYPSYSDNSNITEGIEY
                     NVPGIYDVTLAVNNSCGSNYLIKNDYIYVFTGPVSVYCDSLRNLGPDEVVTTVPVTGW
                     GHIAGHNSQRVKAYADYFEDHSFTQIEGLVVPVRFSDYGSIFSYVKFFIWDGSTTYPD
                     SVLAEKKVLIKDIPDNFYSVITFDEPVEINGPFYAGFSINYPDDDGDGISDDRFVVSV
                     AEPRGGSIVYNTLYVQVTGVWYTCTEKFGFSTSTDIRPVACLVDVEDILADMKINIYP
                     NPCHDDVNIDLSGFSFNEASIEILDITGRTIQSIIAGYPDIYQCSISGEPAGLYFIQI
                     TMDGKRLTRKLSVAR"
     gene            complement(5982..7229)
                     /locus_tag="PLK75_06905"
     CDS             complement(5982..7229)
                     /locus_tag="PLK75_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOY39013.1"
                     /translation="MFTNDIDLMSPVGSFESLMAAIQAGATSVYFGVGKLNMRSRSAI
                     NFTLNDLQEISRICGNHKVRTYLTVNTVIYDDEIDEMHELIIAAKNNNISAIIASDMA
                     VVEYARSNGIEVHASTQLNISNFEAVKFFSQWCDVMVLARELSLKQVAEINRKITEQQ
                     VKGPSGNLIKTEIFVHGALCMAVSGKCYLSLHEFNKSANRGECFQTCRRAYEVFDKET
                     GYQLEIDNEYIMSPKDLNTIGFLNKILNAGVKVLKIEGRARAPEYVKTVTQCYREAIN
                     ACFDGTYSDERIHNWNKRLTLVFNRGFWNGYYLGQPMGEWSMHYGSSASRTKKHIGKC
                     SNYFSKSKVAEFIIETPDTLMPGETILIIGPTTGVVEIMIDEIRVDDQPVHSVERGVR
                     FSVKLFEPVRRNDKLYKYISTGK"
     gene            complement(7456..8997)
                     /locus_tag="PLK75_06910"
     CDS             complement(7456..8997)
                     /locus_tag="PLK75_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39014.1"
                     /translation="MNDKNNIESLFKESLGGFNAKPSEKVWTKVQHGVMPARIKYIVK
                     STFSGFRSTPGSKIWGKINAKLVWHRFLYFKPYSFNIYNVAAIVTITAGTITAFNLTR
                     QKEEKIEKVISHSHYVQPINSFSPENQRLKYTTQSQTGSANILSASESIEQTQLSKES
                     AYLHHNSVAQLAVSAHEGFDTPIPNASSAAAEKSFHPTPERMMFRKIFPFQNYRPDPA
                     YWSNLNFLDYEFRQPVIPDTIGVDFKGDPIVVAGSYLSLEPGYSLISGRTQLKSSNPE
                     IDSHYREKLPYKPEYSYMFGLNVALQEHHLRFIGGINYAQYNETYRQPETKMMVHNSF
                     EYEYNNYTAWVNDTTWILDLEAWIQGDTVYFVRVDSVLCNITDSSLLMLSDTSFTFDT
                     VSYRNRFTYVEIPLIVGYSFNTAGLIITPQTGFIFGFLNGASGSYPTSLEQSYAVNNL
                     YPLRQFIPRWYAGLNLRWYFAGNFGVLAEPWFTADMQSLYKKEIDINRSDFHYGIRFG
                     LSVRL"
     gene            complement(8990..9556)
                     /locus_tag="PLK75_06915"
     CDS             complement(8990..9556)
                     /locus_tag="PLK75_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY39015.1"
                     /translation="MTNMLEIIEGCKRMEKRAQKSLYDMYAPVLMGVALRYSKNREDA
                     EDILQDAFVRIFTHIDQYTDVGSFEGWLRRIITNTAITYYRKNSKYLFHKDYDEIEEI
                     RADDSTSLSLRDDYTREELLFVMNRMPDGFRIVFNLFAIEGLKHKEIAEMLGIDVGTS
                     KSQYHRARVFLQHNLALLSKEKETLAYE"
     gene            9680..10273
                     /locus_tag="PLK75_06920"
     CDS             9680..10273
                     /locus_tag="PLK75_06920"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOY39016.1"
                     /translation="MNILDWAELKLEVNDCRKCRLAKTRKKIVFGMGNPYAGIMIIGE
                     GPGQQEDEQGLPFVGKSGQLLDKILDACGFTRSEHVFIANIVKCRPPENREPAPDERE
                     ACIGYLLRQIEMIDPKIIILLGRTALQGLIGGGLKVTQQRGQWLTWKNIEIMTTYHPS
                     ALLRNPNLKPEAWNDFKKVVEKYIDLIDSEFVPPYSK"
     gene            10404..11243
                     /locus_tag="PLK75_06925"
     CDS             10404..11243
                     /locus_tag="PLK75_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY39017.1"
                     /translation="MKVISIETMRFRVDGGAMFGVIPKTMWSKHHSADQNNMVELACR
                     SLLIDNGERRILVDTGLGKKMNFQELKHYAAENCYSLDLSLNEAGYTSSDITDVINTH
                     YHFDHCGGNTIIDPISGNIIPAFENAVYYISKSHFETSIGPNYREKTSFMQENFVPLK
                     EAGKLRFLDNECELIPGVSVRFFNGHTRGLTAVMITKNSKTLVFTGDMLPAAAFVPLS
                     WISAYDIEPLKTLEEKQQFFKEAIEKDYTLFFQHDALHECGSLIATERGVRINKTFDL
                     ATW"
     gene            11237..11611
                     /locus_tag="PLK75_06930"
     CDS             11237..11611
                     /locus_tag="PLK75_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39018.1"
                     /translation="MVKNLYIALIFAFALLFMQGCSVFQNKQQILTEKITRHQWVVNS
                     YVDNSINEMLEMPEVIYEFQRDGTFSKTYENGEIYIAEWSFSEDADYISIGNNTFKVN
                     TLTNRLMGFSYGDIDIFFVPVK"
     gene            11713..12420
                     /locus_tag="PLK75_06935"
                     /pseudo
     CDS             11713..12420
                     /locus_tag="PLK75_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            12437..14935
                     /locus_tag="PLK75_06940"
     CDS             12437..14935
                     /locus_tag="PLK75_06940"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOY39019.1"
                     /translation="MKNLLLIIPLLMLSAFAFSQQKVKGSVYDSNTKEPLAFVNIVYN
                     DRQQGTTTDMDGHFSIDSKEPVQYLIVSYIGYQKDTILKNEFSGKTDLKIFMSPVATT
                     LGEVTVLPGENPAHRIIQLVIDNRDLNNPEKMQSFAYTSYNKLYATVDLTSIPVIDTL
                     SNYTDSIVFTPGAADTIPDSTINSLNRAKQITDSMYFFMMESVSERKFRSPDKNSEVV
                     IANKMSGLKNPLFFMLATQMQSFSFYSDLITVYDKTYLSPVSKGSTEKYLFIIEDTLF
                     SEEYDTIFVISFRPRKNKNFEGLKGILHINSRRYALQNVMAEPDAQDPMLSVSIQQKY
                     EFVDGTQWFPVQLNTNFTFNSLQLMVDNYSVPLLGIGKSYISNIQINPVFNKNTFDHV
                     VIESDKNANKKTDDFWNEHRPDSLSQREQLTYQFIDSVGEAEKFDKKIAILEYVASGC
                     IPVGFVSIDLNRIMWYNNYEGYRLGFGLKTNDRILHWFSTGGYAAYGFKDKAVKYGGQ
                     LEFYPYKPAEILLGLYYSNDVEISDYYRFRWSNGFLSTESYRDLLITEIDPVEKYLFS
                     AEARVFRYIKANLYCSYNNKHVVDFNRYFLADNPFQTGITENSLPFGFSYYEAGIAIK
                     FAYKEKFLQTPKNNKISLGTDYPILWLNYSAGIPSFDNAQIYHKFQARLSKKFKTTVY
                     GNSYATLVAGWASETTPYTLLYKGPGTQGWLDVSTTFNTMGVNEFISDRFAHLFLKHD
                     FGSLLFRTKKWKPELCVLTNIGWSDYSDKTPIVTSLPGYNKLYYESGIQINYILRAFF
                     IGYGFSVYYRYGPYHLPETIDNFAFKLTLTYSFE"
     gene            complement(15232..15738)
                     /locus_tag="PLK75_06945"
     CDS             complement(15232..15738)
                     /locus_tag="PLK75_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39020.1"
                     /translation="MSRIFIFIVFIFCSIIVAAQSNYEIVTDSASGSKIMLGQCKKID
                     FENELCSGWFYEEFNNYHPDSATVALLKEHIPASGIIFLGTWCSDSRREVPRFFKIVE
                     LAGWCPDNFEVYCLDHKKMLSGIDVASYKIELVPTFIFYDGDTELGRIIETPQTSIEG
                     DLLKIISN"
     gene            15856..16776
                     /locus_tag="PLK75_06950"
     CDS             15856..16776
                     /locus_tag="PLK75_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY39021.1"
                     /translation="MNRRFRLIVIYPLLTVSMILWAYSYLWAKDALVYYNPITLICIR
                     TFISAVFILPVLFILKKQISIKRKHLPALVLLAFFEPFLYFIGETSGLNKVDPASTAV
                     IISTIPLFTPLAAFYFLKEKVTAWNIAGIVLSLIGIAVMVLDHQLNIQISVEGLLFLT
                     LAVVAAIGYSVVIKRLPGNYSVFTIIFYQNAIGAILFIPLVFIFDFQQLLITGWITSA
                     VWPIVKLGVFASTIAFLFYMYALKFMDITKVNIFTNMIPVFTILIMWIVMDEAPEFRK
                     AIGMAVVIVGVFVSQISLGKIRKSDTPAKH"
     gene            16754..17242
                     /locus_tag="PLK75_06955"
     CDS             16754..17242
                     /locus_tag="PLK75_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOY39022.1"
                     /translation="MIHPQNIRQAANQHKKELRELIKKLKRNDKRLLDDKVHRLHYQI
                     FDSYNCTACANCCRSLGPKLTNKDIARLADYLKITDSAFFQKYVVEDEDGDFVFREMP
                     CVFLQSDNLCAVYDIRPKACREYPHTDKTNFHQILDLSLLNAEICPAVYEIFVILQKN
                     NS"
     gene            17239..17892
                     /locus_tag="PLK75_06960"
     CDS             17239..17892
                     /locus_tag="PLK75_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY39023.1"
                     /translation="MNAIQREAVGRLREHLAPLFASENSGHDFMHLLRVEKMAMHLAF
                     PNGADPFICSLAALLHEHDDNKLTHITGGKDEAVRLLMITVENENIRNHVLEIISSLS
                     YKGASVETPMKTIEGMCVQDADRLDAMGAVGIARTFCFCGARNQPIHDPGISPVNHKD
                     EDDYHKAKGTGINHFYEKLLIIKDRMNTEEAKRIANIRHNFMEEYLDRFLKECEGIQ"
     gene            17956..>18881
                     /gene="porQ"
                     /locus_tag="PLK75_06965"
     CDS             17956..>18881
                     /gene="porQ"
                     /locus_tag="PLK75_06965"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOY39024.1"
                     /translation="MKTIKHIVPVLLLFISITIARGQTGGNNIYEFLNIPPAAHLAAL
                     GGNNVSVFDNDLGFVYANPALMHESFSNNLSLNYHNYIADINFAYASYAFHFDKVGTF
                     GAGIYYLNYGDFTHADETGQILGNFSASDYSFNVYYNRPLYERLYAGASLKILYSSMF
                     TYYSSGFAVDLGLHYYSKDSLFSAGVALKNFGTQLKPYFPGNYEPLPYDLQLGVTQRL
                     RHAPFRFSITMQDLLNWSLRYDSPLDDGNVLAADETANDTVNFTIKLNNFFQTVGNAG
                     DEFLRHIIVGVEIVPSQNFYIALGFNYRRRAE"
BASE COUNT         5657 a         3973 c         3798 g         5453 t
ORIGIN      
        1 cagaacaatg ccatgtcagc gtcgagcata tattccattg gcgtttcgca aggcgatatc
       61 aggaatgtac agcgtttgat tttcccgaat cagaccaata ttcccaatgg attttcgtat
      121 tacctaacag cccgtcaggc acccaccgag tttcagcttt caccggaagg cgttttaacc
      181 ggcataccca cacaagaata tgtttcggaa gatttttact ttaaagttac cgacaaccat
      241 aaccttatcg accggaaaac actgcctttt gttactgacg gactgattat gcagttcata
      301 ccccacactc ccgatgacga cattcctgaa tataacgaaa ccgttgatat cgaaattact
      361 gcaacaaacc caatgacttc tgcagtcaca ggtatatcag taacaatgac cagtgccgat
      421 tcttatatta tcatcaccga tgccaatgcc acgctgccaa atattaaccc tggagcaaat
      481 acgaatctac ttaatgcact ccaatttcaa attaatgcaa acacccccga ccaacacacg
      541 gcgctattta atttctctgt atcatccgat caggacacgt ggaattacgc caaaactttt
      601 acagtaaatt cgccgaaaat tcagcccgga gacgttacca ttactgatgg gaacgacaat
      661 gtgcttggtg caggtgaaaa tgctacggtt acggttgaga tatttaacag cggccatgct
      721 ccggcaagcc acataaatat cagtttgtcg acaagcgacc catacctgac tttaaactct
      781 gacccagcgg atattgccaa tctggcggct tcaggtatgg cggaaagcag tttttcagtt
      841 tcggctgctt ccattatccc tgaaacacat atagcggtaa ttacaatgca ggttgtggcc
      901 gataacggat atacctgttc cgagagcttc gaagtaacca tacatacgcc ggtaattacc
      961 gcaggtagcc tcacagtaaa cgacggatct aacttttgtc ttgatgcagg ggaaaccacc
     1021 gagataaaca taccactgaa caatgtaggc cagatatcag ctcataatgt cgttgctcag
     1081 ctctcaacct acgatgaatt cgtaacaata aatgaagcga cttattccac tccttccatc
     1141 aatttcggag agtcggcaat ttcatcgttc aatatttcgg tttcatctga tgcaccattg
     1201 gcacactatg ttgttttcaa ccttcatata acagcggctg atggttttga cgtaaccata
     1261 caggttgcca ccattgtggg tttgatgatt gaaaattttg aatctggcga tttaacgctt
     1321 ttcgattgga taaccacagc ccctgcccca tggtttatta caaccgaaga gccttatgaa
     1381 ggttcctatt gcttgcgttc gggcgacatt aacgacagtc agtcaacaac actgagcatt
     1441 gaaaacactg tggttgtaga aggtgatata agtttctatt ataaagtttc cagtgaaaat
     1501 tattacgatt tccttgaatt tttaatcgac aatgttgtga ttgcctcatg gtcaggaact
     1561 catccatggt cacaatacaa ctacaccgtt gcaacaggca cacacacgtt tgcatggcga
     1621 tacagaaaag atggttcaat cagtaataat tccgactgcg catggataga ctatattgtt
     1681 tttccggcag ttaacgggtt accaccagtt tttgcactgg aacccgccag cgttgaaaaa
     1741 tggatgtact ccgacgaaac cgacaccgat acaatactta tttcaaacgc agggggtggt
     1801 attattgatt acaccgttgg tgttgacttc atcgatgtaa aaagtacaaa aaacataacc
     1861 ggatccacgc taacttgcaa tacaaacagc tttatggcag gcggaactta cgattttgta
     1921 ttcactgcac ataatgcttc aaatgattcg gaatggctta aaagcattac tatcgatttt
     1981 ccagccggtt ttatagtgaa cagttatacc gatttcatag gtggaagcgg tggaaatctc
     2041 atttcgaccg atcctgttgg agatggggca tctgtaacat ggactacagc caatcagtgg
     2101 ggagtaatat acgatggaga gtcggcagtt gttaccgtaa atgttaccat aagcagcgga
     2161 tttgggaata tgctggctga tatcgactat acacttaccg gcgacatcta cggtgcagaa
     2221 ccccacaccc ttgaaggagt gatacttatg gcaaaccagg gcaatttttg gctgacggtg
     2281 attccatttt caggacaact tctttcacag tcggaaaccg aactttatct taacttcaat
     2341 actgagggaa tggataacgg gtactattat gcaaatctga gcattaccca caataatgaa
     2401 gtaagcgttg ttcccattac tctgcatatt gttactgtgg acgttcccga atctgttcag
     2461 gaaaatgtac aaatttaccc gaatcctttc agcaatacgg tgtatattaa tacagccaat
     2521 ctgacagagc caatacatcg tatcgaaatt accgatgttc agggcagact tctttatgtg
     2581 tgcagcaatc gcgaaatctc tgcaaatggc aattgtatat ggttaaatac acaaggattc
     2641 atcagcagca cgggaatata ttttgtaaaa attgtaatgg aacagaaaac cgaaatctac
     2701 aagctgataa aggaataaaa aaagcaggtt aaccctgctt tacgattgcg aaatcaaaaa
     2761 attatttaac cctttccacg tattcgcgtg tcgatgtatc gattttaatt ttgtcgccaa
     2821 cattcacaaa caaaggtacc ataatggtag ctcctgtttc gatggtagcc tgtttaaggg
     2881 tattggtggc agtgtcgcct ttaatccccg gctctgtgta aacaatttcc atttccacgg
     2941 ttgggagcaa ctcgcatgtg agtacctgat cgttttcggc atgaaacatc atttcgacat
     3001 tctgaccttc ttttagcaaa tcggcatttt cgataagttc ttgactcagt gcaatttgct
     3061 cataggtttc gttgtgcata aaatgaaaac ccatatcatc tttatataaa tactgatagg
     3121 gtcttctttc gatacgaact gtgttgattt taacacccga attgaatgta ttgtctatta
     3181 cacgcccatt ttcgagactt ttcagttttg tacgtacaaa tgcaccaccc ttgccgggtt
     3241 ttacatgctg aaaatacaca atctggtaca gtttcccatt aaactctatg gtaaggcctc
     3301 tttttatgtc tgcagtagtt gccataatta ttgttttttc tgaatataaa tttcggtgca
     3361 aaaataatat ttcaacggaa ttttacgaat tataagcacg aaatatatgg taattatcaa
     3421 ttcttgttta actttgttgt tccgcaacca aaatttatgg attgcgtcat gtattcgtaa
     3481 aatgcttaga atatgaaaat taggtttact attttaataa tttttttact ctttctgggg
     3541 tattcacttt ctgcgagtat catttatcac aatgtaaacc ccgacgaagt aatcacacca
     3601 cttgccgctg aatatttcat tgatatgaac agcgatgccg gcaacgattt ctcgatacaa
     3661 aacaatgtgg acgcaggaaa caacattttt gaaatattat tcggatgtat tggggctgga
     3721 gcagatgtag ttggtaccga cataggcgga gttttttgtg tatcggttct caccaacggt
     3781 acaaatatcg gcgcaggcag tgggttcagt ggtcaggccc ccatttcgta tccgtcgctc
     3841 agaactgctt cgtacacttc atggctgggg cagaccaaca aatttatcgg tgtaaaattc
     3901 atgattggtg caaatacata ctatggatgg atacgtatta ctgtttcagg aaccaatgtg
     3961 gttaccgttg tcgactgggc atacaacgac attgctgacg gaaccatcgc tgcaggcgat
     4021 atgggcggtg gaggcactga aaccctcgag gcaaattttc tcgcatcatc ttacaatatc
     4081 aacattggcg agtgtatcag ttttcaggat aattcaatgg gaaatcctac attctggagc
     4141 tggactttcg acggggccga aacacccacc tcaaacctgc agaacccgga gggcatttgc
     4201 tattacacac ccggagtgta tgatgtaacc ctgaatattc agaacgcaac gaataccgat
     4261 acctatatct gcgttggttg catcacagtt attgaccaaa ctattgtacc cattgccaac
     4321 ttcattgccg atcagttggt gataccagca ggttcatcgg tgcagttcac cagtacttct
     4381 cagaacggac cattcacctc gtttgcatgg gcatttgaag gcggattgcc attcagctca
     4441 accgatgaaa cgccgatgct tatttcgtac tccactcccg gtgtttatga cgttcagctc
     4501 acagtagtaa acactcaggg caacgaagat accgaactga agcaggatta tattaaagta
     4561 gtaccgctat cgacagaact accaacggtt aatttcattg cagaccgaac cctgatagct
     4621 ccaggagagt cggtaaactt tcatgattta agccacggtc acccgtatca atgggagtgg
     4681 aattttcagg gaggacttcc tgtgatttca tatgacccaa atccggaggg catagtattc
     4741 ccaaaccccg gcacttacaa cgttcagctg attgccatca acagtctggg atcagataca
     4801 cttatccgca atgcatacat cactgtcaca gataatccca catgcaccgc acctcccgta
     4861 ccgaatttca gggcatcgaa cagattgatt gttgccagca ataaagtata tttcgaagat
     4921 tttaccacaa ataacccatt tacatggaat tggtatttcg aaggcggtta tccaagctat
     4981 tccgacaaca gcaatatcac tgaagggatt gaatataatg ttccgggaat ttacgacgta
     5041 actttggcgg taaacaatag ttgcggaagt aattacctga ttaaaaacga ttatatctat
     5101 gtgttcacag gtccggtttc ggtatattgc gactcgttga gaaatctggg gcctgatgaa
     5161 gtagtaacca cggtaccggt aacaggctgg ggacacattg caggccataa cagtcagcgt
     5221 gtaaaagctt atgccgatta cttcgaagac catagtttca ctcaaattga aggtctggtg
     5281 gttccagtgc gtttttctga ttacgggtcg attttttcgt atgtgaagtt tttcatctgg
     5341 gatggaagta ccacttaccc cgattcggtt cttgcagaaa agaaagtgct tataaaggat
     5401 attcccgata atttctactc tgtaattacc ttcgatgaac ctgtcgaaat aaatggtcct
     5461 ttttatgcag gattcagtat taattatccc gacgacgatg gagacggcat cagcgacgac
     5521 cggtttgttg taagtgttgc cgaaccacgt ggcggatcga ttgtttataa cacactatat
     5581 gtacaggtaa cgggtgtttg gtacacctgt actgaaaaat tcggattctc gacttcgacc
     5641 gacattcggc ctgtggcttg cctggttgat gtggaagaca ttcttgccga tatgaaaatt
     5701 aacatatatc cgaatccctg tcatgacgat gttaatatcg atctaagcgg gtttagtttc
     5761 aacgaagcaa gcattgagat tcttgatatt accggccgaa caatacaaag tatcatagcc
     5821 ggatatcccg atatttacca gtgtagcatt tcgggtgaac ctgccggttt gtattttatc
     5881 caaatcacaa tggatggcaa gcgccttaca cggaagcttt ctgttgcgcg ctgataaaat
     5941 aatgcgctac agggaaatcc cttaagaatc catattccca tttatttccc ggttgaaata
     6001 tatttatata gcttgtcatt cctacgcaca ggttcgaaaa gtttcaccga aaaccgtact
     6061 cccctttcaa cgctatgtac aggttggtca tcaaccctga tttcatctat catgatttct
     6121 actacaccag ttgtcggacc aatgatgaga atggtctctc cgggcattaa cgtatcgggt
     6181 gtttcgataa taaactctgc caccttgctt tttgaaaaat agttgctgca tttccctatg
     6241 tgctttttag tgcgagaagc agaagatcca tagtgcatac tccattctcc catcggctgt
     6301 ccaagataat agccgttcca gaagccacga ttaaacacca gcgtcaaacg cttattccag
     6361 ttatgtattc tttcatcgga ataggttcca tcgaagcagg cattaattgc ttcacgatag
     6421 cattgggtaa cagtttttac atattccggt gccctggctc gtccttcaat tttcagaacc
     6481 ttaacgccgg cattcagtat tttattcagg aaaccaatag tattcaaatc tttaggcgac
     6541 ataatatatt cattgtcgat ttcgagttga tagccggttt ccttgtcgaa aacttcgtat
     6601 gcccgcctgc acgtctgaaa gcactcgccg cggtttgccg acttgttgaa ttcatgcaga
     6661 ctgagataac attttcccga aacagccatg cataatgcac catgaacaaa gatttcggtc
     6721 ttaattaaat tcccagaggg gcctttgact tgttgttcag taattttccg gttaatttcg
     6781 gcaacttgct taagactcaa ctctcgggca agcaccatta catcgcacca ttgcgaaaaa
     6841 aacttcactg cttcgaaatt actgatattc agttgtgttg acgcatggac ttcaatgcca
     6901 ttgctgcggg catactccac aacagccata tcactggcaa tgatcgctga aatattattg
     6961 ttttttgcag caataatcaa ctcatgcatt tcgtcaattt cgtcatcata aataaccgta
     7021 ttaacagtga gataggttct taccttatgg ttgccgcaaa tacgactgat ctcctgcaaa
     7081 tcgttcaacg taaaattgat cgctgaccgg gaacgcatat tcagcttgcc gactccaaaa
     7141 tacactgaag tggctcccgc ctgaatggca gccatcaggc tctcgaaaga gcctaccggc
     7201 gacatcaggt caatatcgtt tgtaaacatt gtattttttt gcaaagctat taaaacatac
     7261 cgatacaaaa gaaagtatcg ttaaaaaagc aattccaaca tgcatcgtta aagaaaaggg
     7321 gctgccggaa gaacccggca aacccctgac aaacacgcta aatttatgat gtagacttgc
     7381 acatttcaaa gactacaaaa cagagaataa ggttgcatct ttattaaaaa aaattagctt
     7441 ctcaaaagat agctgttata acctgacaga taaaccaaac cgtatcccat aatgaaaatc
     7501 gctacggttg atgtcgattt cttttttgta taaactttgc atatcagccg taaaccaagg
     7561 ctctgccaat actccgaaat tgcctgcaaa ataccagcgc aagttcaggc ctgcgtacca
     7621 tcgaggaata aactgccgca atgggtacag attattaaca gcataagact gctccaatga
     7681 agtcggataa ctcccggaag cgccattcaa aaacccgaag ataaaaccgg tttgtggtgt
     7741 aataatcaat ccggcagtat tgaaagaata gccaacgatt aatgggattt caacataagt
     7801 aaacctattt ctgtacgaaa ccgtatcgaa agtaaatgat gtatcggaaa gcattaacaa
     7861 gctggaatca gtgatgttgc acaacacaga atcgactctc acaaaatata cagtatcgcc
     7921 ttgtatccac gcctcaagat cgagaatcca ggttgtatcg ttcacccatg ctgtgtagtt
     7981 gttgtattca tattcaaaac tattgtgaac catcattttt gtttcaggct gccggtatgt
     8041 ttcgttatac tgtgcataat taatccctcc gataaaccgc agatgatgct cctgtaatgc
     8101 tacatttaag ccaaacatat aggagtactc cggtttatat ggcaattttt cacgatagtg
     8161 cgaatcgatt tcagggtttg agctcttcag ctgtgttctg ccggaaataa gactatatcc
     8221 aggttccaga cttaaataag agccggcgac aacaatggga tctcccttga aatcaacccc
     8281 gatggtatcg ggtataaccg gttgccgaaa ctcatagtcc agaaagttca ggtttgacca
     8341 gtatgcaggg tcgggtcggt aattctgaaa tggaaatatt tttctgaaca tcatgcgttc
     8401 tggcgttggg tgaaatgatt tttcggcagc ggctgaactt gcattgggta tgggagtatc
     8461 aaaaccctca tgagcggaga cagccaactg tgcaacagaa ttatggtgca gataggctga
     8521 ttcttttgat agttgtgttt gctcaataga ttcggaagca gacagtatat ttgcacttcc
     8581 ggtctgcgat tgagtagtat attttaacct ctgattttca ggtgagaatg agtttattgg
     8641 ctgaacataa tgcgaatgac ttattacttt ttcaattttc tcttcttttt gtcgggtaag
     8701 gttgaacgca gtaatcgttc cggctgtaat tgtaactata gctgcaacat tatatatgtt
     8761 aaaagaatag ggtttaaagt acaaaaagcg gtgccaaacg agttttgcat ttattttgcc
     8821 ccaaattttc gaaccgggag ttgatctgaa accggaaaaa gttgatttaa cgatatattt
     8881 tattcgcgct ggcataactc catgctgtac tttagtccac actttttctg aaggctttgc
     8941 attaaaacca cccagcgact ccttaaaaag gctctctata ttgtttttat cattcatacg
     9001 ccaaggtttc tttttctttg ctaagtaatg caaggttatg ctgcagaaac actctagcac
     9061 ggtggtactg cgatttcgaa gttcccacat caataccaag catttcggct atttccttat
     9121 gcttaagccc ttcaatcgca aaaaggttga aaactattct gaatccatcg ggcattctat
     9181 tcattacaaa tagtagctcc tctcttgtgt aatcatctcg taaactcaaa gaagtgctgt
     9241 cgtcagcacg tatttcttca atttcatcgt agtctttatg gaaaagatat ttactatttt
     9301 tacgataata agtgattgcc gtgttagtaa ttattctccg caaccagccc tcgaaagagc
     9361 caacatcagt gtactgatcg atatgtgtaa aaattctcac aaaagcatcc tgcaaaatat
     9421 cttcggcatc ttcacgattt tttgaatacc gcaatgcaac ccccattaaa acaggtgcat
     9481 acatgtcgta caacgacttt tgcgcccgct tttccatgcg tttgcagcct tcaataattt
     9541 ctagcatgtt tgtcataatc tactttttat agacttcgta ttagaaaaaa aggttgcata
     9601 caggcaaaaa taaacaagag ttttgaacca ggctaaggtt ttactacatt tgaaatgatt
     9661 ttcgtaattc ttttcaacta tgaatattct cgattgggct gagcttaagc tggaagtaaa
     9721 tgattgcaga aaatgtcgcc ttgccaaaac gcgtaaaaaa attgtttttg gaatgggcaa
     9781 tccctatgcc ggcataatga ttatcggaga aggacccgga caacaggagg atgagcaggg
     9841 tttacctttc gttgggaaat cgggacagct tcttgataag atacttgatg cttgtgggtt
     9901 tacccgcagt gaacacgttt ttattgcaaa cattgttaaa tgccggcctc ctgaaaaccg
     9961 tgagcccgcc cctgatgaaa gagaagcttg cataggttat ctgctgcgtc agattgaaat
    10021 gattgaccct aaaataataa tacttttagg cagaacagct ctgcagggat taattggcgg
    10081 tggtctaaaa gttacccagc aacgaggtca atggctaacg tggaaaaaca tcgaaataat
    10141 gacaacttat cacccttcgg cattgctccg aaatcccaat ctcaaaccgg aagcatggaa
    10201 cgactttaaa aaggtggtcg aaaaatacat tgatcttatt gacagcgaat ttgttccgcc
    10261 ctattccaaa taatctggtc aagtgagagg tttctcccag tatggctaat tcaggagagg
    10321 ctcgccagaa aattcctaaa acgatttcag tctgattaat tataagccaa atctttataa
    10381 ctttgcagcc gaaaatgcca acaatgaaag ttatcagtat cgaaacaatg cggttcaggg
    10441 ttgatggtgg tgccatgttt ggggtaattc ccaaaaccat gtggagcaag catcattctg
    10501 ccgaccaaaa taatatggtt gaactggcat gccgttccct gctgatcgac aacggagaac
    10561 gcaggatatt ggtggatacc ggactgggaa aaaaaatgaa ttttcaggag ctgaagcact
    10621 atgctgccga aaactgctat tcccttgacc tctcgctgaa cgaagcagga tatactagct
    10681 ccgatataac tgatgtaata aacacacatt accatttcga ccattgcgga ggcaatacaa
    10741 ttatcgaccc gataagtggc aatatcatcc ccgctttcga aaatgctgtg tactatatca
    10801 gcaaatctca tttcgaaacc agtatcggtc caaactacag ggagaaaaca tcgtttatgc
    10861 aggaaaattt cgtacctctg aaagaagccg ggaaacttcg atttcttgat aacgaatgtg
    10921 agctcattcc aggagtatca gtaaggtttt ttaacgggca caccaggggg ctgactgcag
    10981 ttatgataac caaaaattcg aaaacccttg tttttactgg tgacatgttg cctgctgcag
    11041 catttgtacc tttatcatgg atttcggctt acgatatcga accattgaaa acactcgaag
    11101 aaaaacaaca attctttaaa gaagccatag aaaaagatta tacgctattt tttcagcatg
    11161 atgcattgca tgagtgtggt tcacttattg ccactgaaag gggagtcaga ataaacaaaa
    11221 cttttgacct agcaacatgg tgaaaaacct ttatatagca ttgatttttg catttgcctt
    11281 attattcatg caagggtgca gtgttttcca gaataaacag cagatcctta ccgaaaaaat
    11341 tacccgacac cagtgggttg taaactccta tgttgacaac agtattaatg agatgctcga
    11401 aatgcccgaa gtaatttacg aatttcaacg cgatgggact ttttcgaaaa cctatgaaaa
    11461 tggtgaaatt tatattgccg agtggagctt ttcggaagat gctgattata taagcatcgg
    11521 aaacaatact tttaaagtga atacacttac aaatagactg atgggattta gctacggtga
    11581 cattgatata ttttttgttc ccgtaaaata gtttgcaagg cattgaaaat attacataca
    11641 acaaacatga aaaaagaaat aaaacttacc cgcagaaccg aaaaagaaaa aactcagaaa
    11701 agcgatggcc ttatccgcct gaacaaatac atttccaatt ctggaatatg ctcaagacgg
    11761 gaggctgaca accatattca aaatgggtct gttatggtga acgggactgt agttaccgac
    11821 ctaggagtaa aggtgaaacc cggagatgaa atacgtttca aaggaaaaaa aatagcccct
    11881 gaacgcaaag tgtatatttt acttaacaaa cccaaggatt ttgttactac aaccgacgat
    11941 ccgcaagggc gaaatattgt aaccgacctg ataaaagacg cctgcagtga aaggatatat
    12001 ccggtaggtc ggctcgaccg tgaaacaaca ggcttgctat tgcttaccaa cgatggagaa
    12061 cttgcaaaag tacttactca cccctcatat gcaaaagtaa aaatctatcg ggctgttctc
    12121 gataagcccc taagtaaaag cgatctcgaa gccattcgcg ccgggtttga gcttgaagat
    12181 gggttcattg ctgccgacca gattgattat gtttcggaag acaaattaga agttggtata
    12241 gagctacatt ccggaagaaa ccgtattgtg cgtcgcattt ttgaacacct tggatatcgg
    12301 ttaaaaaaat tagaccgcgt gtactatgcc ggactcacta agaaagacct gcctaggggc
    12361 aaatggcgtt ttctcacacc tgccgaaatt tcgatgctta aaatgggggc ttaccgctaa
    12421 agcatcaagg gcgtttatga aaaatttact gctgataata cctttgttga tgttgtcggc
    12481 atttgctttt tcccaacaaa aagtaaaggg gtctgtttat gattccaata caaaagagcc
    12541 acttgctttt gtgaatattg tttacaacga tcgtcagcag ggaaccacaa ccgatatgga
    12601 tgggcatttc agtattgata gcaaagagcc ggttcaatat ctaatagtga gttatatcgg
    12661 ctaccagaaa gacaccattc taaaaaatga attcagcggt aaaacagact tgaaaatatt
    12721 tatgagtccg gttgcaacaa ctttgggaga agtaaccgta ctacccggtg aaaatccggc
    12781 acataggatc attcaactgg taattgataa ccgcgacttg aataatcctg aaaaaatgca
    12841 gtcgttcgct tacacctcct ataataaact atacgctacc gttgacctga cttcgattcc
    12901 tgttatcgat accttgagca actatactga cagcattgtg tttactcccg gagcagccga
    12961 taccattccc gactcaacca ttaacagcct caaccgcgcc aaacagatta ccgacagcat
    13021 gtacttcttt atgatggaat cggtaagtga acgcaagttc aggtctcctg acaagaacag
    13081 tgaggtggtg attgcaaata aaatgtcagg attgaaaaat ccgttgtttt tcatgcttgc
    13141 cacgcaaatg cagtcgtttt cgttttacag cgaccttatc acggtttacg ataagactta
    13201 tctgagtccg gtaagcaaag gcagcaccga aaagtatctc ttcattattg aggacacact
    13261 ttttagcgaa gaatacgaca ccatttttgt aatttcgttt cgcccacgaa aaaacaaaaa
    13321 ctttgaagga ctgaaaggga ttttgcacat caactctcgt aggtatgcac tacaaaacgt
    13381 aatggcagag ccagatgcgc aggatccaat gctttcggtg tcgattcagc agaaatatga
    13441 gttcgttgat ggcacgcagt ggtttccggt gcaactgaat accaatttta cctttaactc
    13501 gcttcagctg atggtagata actattctgt cccgttgctt ggtatcggca aaagctacat
    13561 cagtaacata caaataaatc ctgttttcaa taaaaacaca tttgaccatg tagtaattga
    13621 aagtgataaa aatgccaata aaaaaactga tgatttctgg aatgagcacc ggcctgacag
    13681 cctgagccaa cgcgagcagc ttacctatca gtttatcgac agcgtgggcg aagcagaaaa
    13741 atttgacaaa aaaattgcaa tactagaata tgtggcaagc ggttgcattc ctgttgggtt
    13801 tgtaagcatc gacctgaacc gtattatgtg gtacaacaac tacgaaggat atcggctggg
    13861 gtttggcctt aaaaccaacg acagaatcct tcattggttt tctactggcg ggtatgctgc
    13921 ttatggattt aaagacaagg cagtcaaata tggagggcag ctagagtttt atccttataa
    13981 acctgcagaa atattgcttg gtttgtacta ttccaacgac gttgaaataa gcgattatta
    14041 ccgcttcaga tggagcaacg gttttttgag taccgaatca taccgcgacc tactaattac
    14101 agagattgat ccggttgaaa aatacttgtt cagcgctgaa gctcgtgttt tccgatatat
    14161 taaagcaaac ttgtattgtt cttacaacaa caagcatgtt gtcgatttca accggtactt
    14221 tcttgctgat aacccatttc aaaccggaat caccgagaac tcattgccct ttggcttttc
    14281 atattatgaa gcaggaatcg ctataaaatt tgcttacaaa gaaaaattcc tgcaaacacc
    14341 gaagaataac aagatcagcc ttggtacgga ttatccgata ctatggttaa actattccgc
    14401 aggaattcca tcctttgata acgcacagat ttatcataaa tttcaagcac ggctgagcaa
    14461 aaaatttaaa accactgttt acgggaactc ctatgcaacc ctagtagcag gctgggcaag
    14521 cgagacaact ccatacaccc tgctttacaa gggtcctgga acacaaggat ggcttgatgt
    14581 ttctactacc ttcaatacta tgggtgtaaa tgaatttatc tctgatcgat ttgcgcattt
    14641 gtttcttaag cacgattttg ggagcctgtt gttccgtaca aaaaaatgga aacccgaatt
    14701 atgtgtactc accaatatag gttggagcga ttactccgat aaaaccccta tcgtaacatc
    14761 gctacccggt tataataaac tgtactatga atcgggtatt cagataaact acatactacg
    14821 tgcttttttt atcggatatg ggttctcggt gtattaccgc tacgggcctt accacctacc
    14881 ggaaacaatc gacaactttg cttttaaact aacattaact tatagttttg aatagtaccc
    14941 ggcaataatc aacaactacc aatgacgtat tattactact gggaatacag aatgcagaat
    15001 acagaagggg cgaatggcaa ttcaaccgca taattctctt gctggagttt tcgttcactc
    15061 tcgtaacatc atgcctctat cgaccaacga aatgccccct tctgaattct gtattctgtc
    15121 tcctgtattc tgattccaga taccaggtca ttttatattg cttttattgc cgcaataatt
    15181 agccctcaac aaggcagaga ttgggctaaa atgtgtataa ctgagtcctt tttaattcga
    15241 aataatcttt agcagatctc cttcgattga tgtttgagga gtttcgataa tacgtccgag
    15301 ctctgtatcg ccatcataga aaataaatgt gggaacaagt tcaattttgt atgaagcaac
    15361 gtcgattcca gacagcatct ttttatggtc gaggcaatac acttcgaagt tatcggggca
    15421 ccaacctgca agttctacaa ttttaaaaaa acgaggaact tcgcgccttg aatcgctgca
    15481 ccaggttcct agaaaaataa ttcccgaagc aggaatatgt tccttcaaca aagcaacagt
    15541 cgcactgtcg gggtggtaat tattgaactc ctcatagaac cagccactgc aaagctcgtt
    15601 ttcgaaatca atctttttac attgccccag catgatttta cttccgctgg cagaatcggt
    15661 aacaatttcg tagttactct gtgctgcaac tattatgctg cagaaaataa atactataaa
    15721 aatgaaaatt ctgctcataa gtaattttta tgcaaatata tcgttttctg aattcaacaa
    15781 acctgaaaat taattatggc aggtgtaaaa taatttcgat acaattaaaa gtcttactgt
    15841 atctttgcac gcattatgaa ccgcaggttt cgccttatag taatttaccc gctgcttaca
    15901 gtatcgatga tattgtgggc gtactcgtat ttgtgggcaa aagacgcttt ggtttactac
    15961 aatccgatta cactgatttg tatcaggact tttatctcag ctgtttttat tttaccggta
    16021 ttgttcatat tgaaaaagca gatttcgatt aaacgtaagc atttgcctgc attggttctc
    16081 ttggcatttt tcgaaccatt tttgtatttc attggagaaa caagcgggct gaataaagtt
    16141 gacccagcct caacggccgt aataatttct acaatccctc ttttcacacc gttggcagca
    16201 ttttattttc tgaaagaaaa agtgactgca tggaatatag ccggaattgt gctttcatta
    16261 attggtattg ctgtaatggt gcttgatcat cagctgaata ttcagatttc ggttgaaggg
    16321 ctgttatttc tgacacttgc tgtagttgct gccataggat acagtgttgt aataaagcga
    16381 ttacccggaa attattccgt ttttaccatc attttttatc agaatgccat tggagccatt
    16441 ctgtttattc cactggtttt tatatttgat tttcaacagt tactgattac cggctggatt
    16501 acttctgcag tatggcccat tgttaaactt ggcgtattcg catcgacaat tgctttttta
    16561 ttctacatgt atgccctaaa atttatggat attaccaaag tgaatatttt taccaatatg
    16621 ataccggtct tcaccatatt aattatgtgg atagtaatgg atgaagctcc ggagttcaga
    16681 aaagcaatcg gtatggctgt cgtgatagtg ggggtatttg tgtcgcaaat aagtttaggt
    16741 aaaatcagga aaagtgatac acccgcaaaa cattaggcag gcagcaaatc agcacaaaaa
    16801 agaacttcgt gagctgataa aaaagttgaa gagaaatgat aaaagattgc tcgacgacaa
    16861 ggttcacagg ttacactatc agatttttga ctcatataac tgtactgcct gcgccaactg
    16921 ctgccggagt ctcggtccaa agcttacaaa taaagatatt gcacggttgg ctgactattt
    16981 gaaaataaca gattctgcat tttttcaaaa atatgttgtc gaagatgagg atggtgactt
    17041 tgttttcagg gaaatgcctt gtgtttttct gcaatctgac aatttatgcg ctgtatatga
    17101 tatcagaccc aaggcatgca gagagtaccc gcataccgat aaaaccaatt ttcatcagat
    17161 acttgattta agtttgctaa atgcagaaat ctgccctgct gtgtacgaaa tttttgtaat
    17221 cttgcaaaaa aacaactcat gaatgcaatt cagcgtgaag cagttggtcg gcttcgtgaa
    17281 catcttgctc cgcttttcgc atctgaaaat agcggtcatg acttcatgca tcttttgcgg
    17341 gtggaaaaaa tggccatgca ccttgctttt ccgaatgggg ccgacccgtt tatctgttca
    17401 ttggcagccc tgctgcatga acacgatgat aataaactca cccatataac cgggggaaaa
    17461 gatgaggctg taagattgct tatgattaca gtagaaaacg aaaacatccg caatcacgta
    17521 cttgaaataa tcagctccct ttcatataaa ggcgcctcgg tggagacccc aatgaaaacc
    17581 attgaaggaa tgtgcgtgca ggatgccgac aggctcgatg ctatgggagc agttggtatt
    17641 gccagaacat tctgcttttg tggtgccagg aatcagccta tacacgaccc gggcatttca
    17701 ccggtaaatc acaaggacga agacgattat cataaagcaa aaggcacagg aatcaatcat
    17761 ttctacgaaa agcttctcat cataaaggac agaatgaata cagaagaagc caaaagaatt
    17821 gccaatatcc gccataactt tatggaagaa tacctcgata gattcctcaa agaatgtgaa
    17881 ggaatacaat aatgggtaaa ggaatgcgtt tgattattta atatatttgt ctcaaacaaa
    17941 ttaaattcaa gctgcttgaa aacgataaaa catatagtac cagttttatt actattcatc
    18001 tcaattacta tagcacgagg ccaaaccggt ggaaacaaca tatacgagtt tttgaatatc
    18061 cctcctgctg ctcaccttgc agcacttggt ggaaataatg tttctgtttt cgataatgat
    18121 ctggggtttg tctatgctaa cccagcccta atgcacgaga gtttcagcaa caatcttagc
    18181 ctgaactatc ataattatat cgccgacatt aactttgcct acgcttctta tgcctttcat
    18241 ttcgataaag ttggcacttt cggagctggc atatactact taaattacgg cgattttact
    18301 catgcggatg aaacaggaca gatccttggg aacttttctg cttccgatta ttcattcaac
    18361 gtgtattata accgacctct atatgaacgc ctgtatgccg gagcaagcct aaaaatacta
    18421 tactcatcga tgtttacgta ttattcttcc gggtttgcag tagatctcgg gttgcattat
    18481 tattcgaaag acagcttatt ttctgccggg gttgctttaa agaatttcgg cacacaactc
    18541 aagccttatt ttccagggaa ttatgaaccc ctgccctacg accttcagct tggtgttaca
    18601 caacgccttc gccatgcgcc atttcgcttt tcgataacca tgcaggattt gttaaactgg
    18661 agtctgcgtt acgatagtcc gttggatgac ggaaacgttc ttgcagccga tgaaacagca
    18721 aacgatacag tgaattttac aatcaagctg aacaactttt tccaaacggt tggcaatgct
    18781 ggagatgagt ttctacgcca tataattgtc ggtgtcgaga tagtaccatc acaaaacttc
    18841 tatattgccc tcggcttcaa ttatcgccgc cgtgcagaaa t
//
LOCUS       DAOOQP010000052        18718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3598_length_18718_cov_5.892783, whole genome shotgun sequence.
ACCESSION   DAOOQP010000052 DAOOQP010000000
VERSION     DAOOQP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3598_length_18718_cov_5.892783"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..935
                     /locus_tag="PLK75_06970"
     CDS             177..935
                     /locus_tag="PLK75_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOY39025.1"
                     /translation="MKKLFFVFAMLIFLLSAGFGQTKPTIEWADIPAGTFTMGSPTSE
                     VDREDDETQHQVTLSAFKMSKYEVTFEQYDLFCEATGRSKPDDEGWGRGNRPVINVSW
                     DDATAFAEWMGCRLPTEAEWEYACRAGTTTPFNTGNNLTTAQANYNGNYPYNNNAKGK
                     YRQKTMPVGSFAANAYGLYDMHGNVWEWCSDWYGDYSTSAQTNPKGASSGSDRVIRGG
                     GWGDGAAYCWSAFRYGSTPDYRYYIFGFRLVSPK"
     gene            1083..1301
                     /locus_tag="PLK75_06975"
     CDS             1083..1301
                     /locus_tag="PLK75_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39026.1"
                     /translation="MQIRIFNIPISDNGEMEAEMRCIELVEMNRFLAASKVLEIEQPI
                     TAVSVNAYCKKYPTIVERSEAIYLQVDE"
     gene            complement(1614..4253)
                     /locus_tag="PLK75_06980"
     CDS             complement(1614..4253)
                     /locus_tag="PLK75_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY39027.1"
                     /translation="MKKIYILLIGVLFTCSVTSQEIKPDRKYVNLFYDAEAYMYEANY
                     EEALSLLLKLDEMDPDNANVRYKIGICYLNSRLNKKNALEYLLFSAENINKDYKADNH
                     RERKAPVECLLYLGQAYRVNYKFNDALTTFNELLTRLTPQGKFDADLVAQTKREIEIT
                     NNAIYFTSHPVDASIKNMGSIINTEFCDHSPVIDMNEEYLIFTSKRPRAGEERVNQDE
                     DIFLSKNKNFVWTEPKRLEDNVNTYKNEASIGFSADAKTLFIFRSEGPNYSNVMMTMT
                     SDDLLWSDPKLLSADINTKFRETHACLSPDGNTLYFVSNREGGVGGRDIYKMNLLPDG
                     SWSPPQCLSTDVNTIYDEETPFIHPDGITMFFSSKGHESMGGYDVFRTTIEGDNKFSK
                     PENIGYPINTPDDEVSYVVNLDGRRAYLASSKDDTYGDLDIYEITQTGVYINTMVVYN
                     GQVADINNQVPKDLVIRVNDKTNNTVQGVYRTGSTDGNYYLILQPGHEYLIEYEADGY
                     LATTKTVSPERKDVNSFMEKYIPVPLDRVTLQSYKYHEYVYFVSDSIPYTIESVTALN
                     DVAAKNSESDRLIVNINYLPEKEKEEISAKRAVYITDYLSTKGVEKTDIYLNGAFPAG
                     YQDIYCLDMKETQEPVVITHLPLDTTDTGKKYGDTVYIDPVYFAFDRDEVRSQFFDSL
                     NVLASYMKANPTASVEIGGHTDWYGTNEYNYLLSYRRSKHVKDYLVGKGVNPENLITT
                     KFGEGTPIAENANPDGSDNPLGRQYNRRVEFKVLSQGTESFLVARKIVVPPSAGSNTK
                     GTGSDGTHTQPKFTIQIMAVHNPVPDNYFADLIGVQLQKGQDGWYRYYVGSFDSYNQA
                     KESLDKLKSMGYDPFIRRLSFFE"
     gene            complement(4317..6206)
                     /gene="dxs"
                     /locus_tag="PLK75_06985"
     CDS             complement(4317..6206)
                     /gene="dxs"
                     /locus_tag="PLK75_06985"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOY39028.1"
                     /translation="MGVLDKINYPEDIRKLDIKELPGLCDELRSFIIDAVSCNPGHLG
                     ASLGVVELTVALHYVYNTPIDLLIWDVGHQAYGHKILTGRKEVFHTNRKLKGISGFPS
                     PKESEYDAFGVGHSSTSISAAIGMAVASKHLGESRKVIAVIGDGAMTAGQAFEGLNNM
                     NIQNTDILIILNDNKIAIDPSTGSLQEYFTDISTSKTYNRVKNDVWRMLGKVSKFGPN
                     AQGIAQRVDNAIKSFVMKSSNLFESLKIGYFGPVDGHDVFYLVKILTDMKGLSGPKLL
                     HCITTKGKGYVPAEIDQTIWHAPGEFDIATGTIIQEKNIDKKPPRFQDVFGETIVELA
                     ESNSKIFGITPAMPTGCSLNLMMEKMPHRAFDVGIAEQHAVTFSAGLAIRGMIPFCNI
                     YSTFAQRAYDQIIHDVALQNLHVVFCFDRAGLVGNDGATHQGAFDISYLRCIPNMVIA
                     APMDEIELRHMMFSAQLEKNNFPICIRYPRGRGIYNDWKKPFEEITIGSGRTIREGND
                     IAILSFGSIGIKVTEACKRLAEQGISTAHYDLRFVKPLDTELLASVFKKFKKILTVED
                     AALIGGFGSSILEYIADNGVDVKVARLGIPDIFVEHGNQSDLYSLCHYDIQSIVEEVI
                     KLNRK"
     gene            6361..7389
                     /locus_tag="PLK75_06990"
     CDS             6361..7389
                     /locus_tag="PLK75_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY39029.1"
                     /translation="MSKNVFPLIAVAVMIAAVVSCVPARKFEEVNSLKKQCEQERSIL
                     KGDNEKLTTENNELKNEMERIKITMSALERDTAIKGNAYRTLTIQYDKINQLYDQLLE
                     NQSKLREGADAETQKALAMLQQTRDELQKKEDELKLLEAQLNKERANLEALKAQLDLK
                     EKEIDEKNAKVKELQAILASQDSAMNALRDKVATALRGFEGEGLTVTMKDGKIYVSLD
                     EKLLFKSGKWDVDPKGEAALIELAKVLEKNPDINIMIEGHTDELAYHPNGNIEDNWDL
                     SVKRATAIVKILLKNSKIDPVRLTASGRGQYLPVDKAQTPEARAKNRRTEIILTPKLD
                     ELYKMFNE"
     gene            7478..8482
                     /locus_tag="PLK75_06995"
     CDS             7478..8482
                     /locus_tag="PLK75_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39030.1"
                     /translation="MRNPIQYCRYFSARKIINLSKAGLSFAVNRIFRKHPSFRNHYAV
                     SIETTAVCNLQCPACTLGSGELLRDNRYMDYEVFKRIVDELRPNIINANLYFQGEPLM
                     HPEIENLISYAEKNRIHTTISTNGGLLSLKASRIASAGLSRLIVSVDGITPDTYERYR
                     VNGDFSVLCEGLNSLMNLKKTYKKFTTVTEAQFIVMRHNEHEIHDFFIWAKKIGFDRA
                     HLKSLQVNRHKDVELVPAKSCYARYKVDESGNLQIKSGLKNACYRLLSTVVITVDGDV
                     VPCCFDKNAKYSFGNINQRSINEILASEKAKDFRKKVFNDRKSVDICLNCTEGLNIRY
                     "
     gene            8492..9013
                     /gene="folK"
                     /locus_tag="PLK75_07000"
     CDS             8492..9013
                     /gene="folK"
                     /locus_tag="PLK75_07000"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOY39031.1"
                     /translation="MQRDVIYHTLFLSVGGNVGNRLHYMRMVYLEINSSIGTIINASP
                     VYLSEPWGFMHRRYFTNAVFHVETALELCEILPKLHKIEANLGRIRSAATGYIARTMD
                     IDIIAMDDTVYNEENVTLPHPRMRDRLFVLLPLFDIAPQWVHPRTGENIQELIRKCSD
                     TGKIRKTMLCYYD"
     gene            9006..9443
                     /locus_tag="PLK75_07005"
     CDS             9006..9443
                     /locus_tag="PLK75_07005"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOY39032.1"
                     /translation="MTEEIYMKMAYDEAMKAYDEGEVPIGAVVVCENKIIARCHNQTE
                     MLHDVTAHAEMLAITAAENYINGKYLDKCTLYVTLEPCVMCAGALSWSQIGSIVYACS
                     DEKRGYSQFSGNILHPKTVVRSGVLEEICRNLLLEFFREKRKL"
     gene            complement(9438..10808)
                     /locus_tag="PLK75_07010"
     CDS             complement(9438..10808)
                     /locus_tag="PLK75_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOY39033.1"
                     /translation="MAVLRIVGGIGWYVFSEKEQPQAVRKATEQIYEMNTIIIIVIAV
                     LFSALFSGLEIAFMSSNKLRIELDKKQGGFSPKIISFFADNPSQYISTMLVGNNIALS
                     VYSIYAAKLIEPFLLNITDSAFAILITQTLCATMVILLLGEFLPKMMFRNTSNDALKF
                     FAFPLLIIYYVLYPVVILFVGLSGLIIRVLTGVKIHKADEERVFGKIDINQTLSEIGE
                     DPYSNTETQNRDLKIFQNALDFSDIKLRDCMVPRTEICAFQIDGNPDELKNIFIESGY
                     SKILIYKESIDEITGYVHVLDLFRNAQKVRNMLNPVLIVPETMPAYRLLENFISNNKS
                     IAVVVDEFGGTSGIVTIEDIMEEIFGEIEDEYDVSELIEKQVSENEYIFSARLETEDL
                     NNKYGLNLTESSEYKTLAGYIMYFHGSFPQIHNEITIIDNRQKLIVKILKVSASKIDL
                     VSLRIL"
     gene            complement(10911..11468)
                     /gene="lptC"
                     /locus_tag="PLK75_07015"
     CDS             complement(10911..11468)
                     /gene="lptC"
                     /locus_tag="PLK75_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOY39034.1"
                     /translation="MKYKTTYLANIIAICVVLGSCSNNIEEVNELTSVTTLPATTVKN
                     IETIYSDSAVVKLKLTAAEYRHFESGPEKYDEYPQGLLVEFYGSHLNVVGKLSCQYAR
                     YFPDKNLWEVRTNVEAMNLEKDEKINTELLWWDMNLEEIYSDKFVRITTADEIIYGDG
                     FRSNQDFSSWKIIKPKGTITINDEE"
     gene            complement(11487..12833)
                     /locus_tag="PLK75_07020"
     CDS             complement(11487..12833)
                     /locus_tag="PLK75_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39035.1"
                     /translation="MKTVFFKRAFLIMLGSALGSMTLNAQEDSNFGSNPDECKVKLST
                     YTEFFKQENYHDAYPAWTWCIKNCPASTKNLYIHGPTILDYLISNAKDQNTKESYIDT
                     LMMVYDMRIKYYNEEGKVLGRKANDLLKYRPDSIKQAYNIYKRSIELTGNSSESSVLG
                     YFMNVSVILLKSGDLSKETVVENYSTVSEILDKQIAAAAGDDKTVERLSKINEKVEEM
                     FVNTPAADCEAIITIFTPKYEANKTDLVLLQKIAKLIEKTKNQDCLTTDLYASVSESL
                     YVLEKDAESAHKIAKVYFIKNNTEKAEFYYNEAIRLQSDEKQKADLYYELATLYFGNL
                     KMFAKSRDFARKALASDPNYGKAYLLIGKCYASGGSGCGETELDKVAVYWVVVDQFQK
                     AKSADPSVSEEANELIRKYSGYFPTQEKAFWYNVSEGQPYTVGCWINESTTARFIQ"
     gene            complement(12836..14113)
                     /locus_tag="PLK75_07025"
     CDS             complement(12836..14113)
                     /locus_tag="PLK75_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39036.1"
                     /translation="MLNKLIFVLFLITAAYLAEAQNSSPYSRYGLGDLNSRSFGPSRA
                     MGGTAAAFSSGRNLNVVNPASIASVDSMCFIFEFGLESGIRYFKTTNPDLSALSNDTK
                     LSYLAFGFPVTRWWASSIGIMPFSSVGYSIRSSDSTFNVPKYYYYEGSGGINQVYWGN
                     AFLPFRNFRIGVNAYYLFGMQNRSNSVIFSDDSGAYVNVLEENIVYVNDFCFSAGLQY
                     DFALGSKSKLSLGAVFSYPNNLNSRRDVLVTNSLSVGSSSTVDTIYNEDNQRGTVSMP
                     LSAGGGLLYNFDDKLKLAFDYNMQNWSDAKFFNQSDSLGNSNCFRFGAEYLPAGPSRA
                     TSKYSQRVAYRFGAFYNNTYINFATYGEQIADFGISFGFGLPLNRSKTSFNISIELGQ
                     RGTTRNSLVRETYANLGVNLTLSDVWFIKRKFD"
     gene            complement(14103..14828)
                     /locus_tag="PLK75_07030"
     CDS             complement(14103..14828)
                     /locus_tag="PLK75_07030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOY39037.1"
                     /translation="MDLVADIGNTKTKLRYFNNHGAIVSSVDISAGEEIKLKNSGFIV
                     PDRAIICDVAARSAALSGLLRSKGVIPIMMSAELCTPLSIQYKTPATLGGDRIAAAVG
                     ANFLYPAKNILIIDAGTAITYDYVDKNAAYLGGNISPGLILRYKSLSNYTGKLPLMAP
                     VETWQETGTSTAEAIVSGVQNGILYEIENYIRDWYNKTVDSMVILTGGDISYFVNSIK
                     SPIFVNFELVPIGLKRILDLNAE"
     gene            14948..17077
                     /locus_tag="PLK75_07035"
     CDS             14948..17077
                     /locus_tag="PLK75_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOY39038.1"
                     /translation="MAILQKIRNRAGIFVIIFVGVALFLFIIDPTTFNSLFHKNPTDI
                     ASINGHDIKYEEYLKIAQEHEDFLKVSQQTDNLSPETLESVQQQAWMDLLRMYILDVN
                     FEDLGITVSDEELEDMLWGKHLSPIIQQNFTNPQTGLIDTSMIKNYFQNAEKDETGKQ
                     LFIADYLKKAMVYEYQYGRLHIKYRTLIEKGLYVPTFIAKDDYYSKNNKADILVVARN
                     YKDVDDAVIKVSDADIKNYYDEHIERFQILEANRDIDFVIFDVFPSKEDTLAALKDIK
                     TNSKQFCTIEAETVTDFLNSYSDSLYQEKYYRPEEFAFVGGFDPATMKTGDTSEIFHF
                     QGSFIQARVLDVSTRPDSVKASYMFIMADTTKSIEQVKVLADSIYNVLVKGADFAPLA
                     MQYSDHPASKEKGGDLGWLTESMLGDDKELKDATFLSKKGEIKKIETEFGVHIIKIDE
                     QTMLLQKFSIAYLYKNIKYSTQTAQMRFSQASEFASKNNTAAKFDKACIDLQLTKRMG
                     NAIKENDMKIPGLVNPRSIIKWAYGEEIEKGAVSPVFELNDMYVVAKVATIREKGTAA
                     IEDVKEQIEPIVVKKKKAALLTEEFNKDLSKGESLEAIALKYNKKLDTISNITFNSMS
                     LPGYGLEPNVTGTASVLDKGKTSKPIEGNNAVYVIAVVNKVPAPAKTDFKDEQLYLMR
                     SAASKTYQVFEVLKKIADIKDYRSTWM"
     gene            17194..18636
                     /locus_tag="PLK75_07040"
     CDS             17194..18636
                     /locus_tag="PLK75_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOY39039.1"
                     /translation="MKTFFTACVFVWICSLGLSALPVSVENATKGALHFISCEKKLNH
                     CQIVDFQHITVGGETVAYAFNLSPMGYIVMSSSYYLPPVIAFSTESGFGELGEHNPLY
                     TLIVSDLPLRMKYSSDIEAGYARENILQWEALLNQNPRSKGEPQQWPEGQEGWIQSSW
                     NQNAPYWNMCPVDPVTHVRCYTGCPATAMAMIMDFHATTNNVQFTDEDDYYHSYAGRN
                     YWIDDDFEANGFPSFPDLNKYLDTLNMHYQLKSVITSNDKAALSFACGIAAHQVYTSQ
                     GSGTFAVSQAYDAYMRFGCITAVLMYPEEEGVFDSIILNIQHALPVHLAVVDESNSSG
                     HNLVIDGYRTDNYFHLDYGYGEYYNGWYLIPSEIQMGLTVIEGVIVNIMKQNTSGIYF
                     PIAEQSNIEVFPVPANDFCNIQCCKLSDGNAKIAVCNGFGAEILKTSVDMFDSENRLR
                     IDTGSLPEGMYFVKINSGAETKTAKIIVIH"
BASE COUNT         5663 a         3676 c         3820 g         5559 t
ORIGIN      
        1 aaaataatat tttgaatata tgtaataaaa tttaaatttg aaaaatgatt tcaacaatca
       61 aataggtatt ttgaaagata tataaaagac tttgatatcg aatttgttaa ttgatacaaa
      121 accggagcaa gccgaaaatc ctttaaagag taggcaaggt ttaaaaacga acaaatatga
      181 aaaaattatt ttttgtcttt gcaatgttga tattcttact gtcagcgggt tttggacaaa
      241 caaagccaac tattgaatgg gcagatatac ctgccggcac ctttaccatg ggcagcccta
      301 cgagtgaagt tgacagagaa gacgatgaaa cccaacatca ggtaaccttg agtgcgttta
      361 aaatgagcaa gtacgaagta acctttgagc agtacgattt gttttgcgaa gctacaggca
      421 gaagtaaacc ggatgatgaa ggttggggac gtggtaaccg ccctgtaatc aatgtaagtt
      481 gggatgatgc cactgccttt gccgaatgga tgggttgccg cctgcccacc gaagccgaat
      541 gggaatatgc ctgccgtgca ggaacaacca caccgtttaa tacaggcaac aatctcacaa
      601 ctgcacaggc aaactacaat ggcaattatc catataacaa caatgcaaag ggcaaataca
      661 ggcaaaagac tatgccggta ggaagttttg cagccaatgc ttatggtttg tacgatatgc
      721 acggcaacgt atgggaatgg tgcagcgatt ggtatggcga ttattcaaca tcggcacaaa
      781 ccaatcctaa aggtgcatca tcgggctctg accgcgtgat ccgtggcggg ggctggggcg
      841 acggtgcggc ctattgctgg agtgcgttcc gctacggcag caccccagac taccgctact
      901 acatttttgg cttccgcctt gtctccccca agtaaacagt ggttagtcct ttctttcctt
      961 taaagctgaa tgagttggct aaaagtgagg aagggacaac cgaacaaaga gccaacgaat
     1021 aataaatacc gcgacggttg ttgagcttgt cgaaacaagc ggtcgaaatt ttattttgaa
     1081 aaatgcagat acgaattttc aatataccaa tatccgataa tggtgaaatg gaggcagaaa
     1141 tgcggtgcat tgagcttgtc gaaatgaatc gttttttggc tgccagcaag gtgttggaaa
     1201 ttgaacaacc cataactgca gtaagcgtca acgcttactg caagaaatac cctaccatcg
     1261 tagaacgcag tgaagcgatc tacctccaag tagacgaata atttaccgcc catcgaaatg
     1321 cagattgctt tgtcctcgtt cctcgtccgc gcaatgacgg gcggctcccc tccggacact
     1381 gaggcactcg aagtgtccga tgtagtaagc actcgaagtg tctggttgca tttcgccttt
     1441 gaagtattgt aacgtaaagg ttgcttcgag agcctcagca acctttcact cgaagtgtac
     1501 ggatgccaac ctgtatatac aaaagtggtt atcaaccgct ttctgatttt agctactgac
     1561 taaaaaaata gagaaccgct gccggttctc tattttttta cgattattga gttttattca
     1621 aagaaactga gtcttctgat aaacggatcg tagcccattg atttaagttt gtcgagcgat
     1681 tctttggctt gattatagga atcgaaggag ccaacataat acctgtacca tccatcctgc
     1741 cctttctgaa gttgaacacc gattaaatcg gcaaagtagt tatcgggaac agggttatgt
     1801 actgccataa tctgtattgt aaacttcggc tgggtatgag taccgtcaga tcctgttcct
     1861 ttggtattac ttcccgcact gggtggaacc acaatcttac gtgctaccag aaatgattca
     1921 gttccctggc ttagtacttt gaattcaaca cggcggttat attgcctgcc caaaggatta
     1981 tcgctaccat cggggtttgc attttcggca atgggtgtgc cttctccaaa ctttgttgta
     2041 atcaggtttt cgggattaac accttttcct accaagtaat cttttacatg tttcgatctg
     2101 cggtacgaca gaagataatt gtattcattg gtaccgtacc agtcggtgtg tcctcctatt
     2161 tcgacactgg ctgtggggtt ggctttcata taactggcca gaacattcag gctgtcgaaa
     2221 aactgcgacc ttacttcatc gcggtcgaat gcaaagtaca ccggatcgat atatacagta
     2281 tcaccatatt ttttacctgt gtcggtagta tcaaggggta ggtgggtaat tacaacgggc
     2341 tcctgagttt ctttcatatc aaggcaatag atatcctgat atcctgcagg aaatgctccg
     2401 ttgagataga tgtcagtttt ttcaactcct ttggtgctaa ggtaatcggt tatataaacg
     2461 gctcttttgg cagaaatctc ttctttttct ttctctggca aataattaat attaacaatt
     2521 aatctgtcgg attcagagtt ttttgcagct acatcattca atgcagtcac tgattcaatt
     2581 gtatatggaa tactatcgga gacgaagtaa acatattcgt gatatttata cgactgtagc
     2641 gttaccctgt cgagaggaac aggaatgtat ttttccataa aggaattaac atctttccgc
     2701 tcaggtgaaa cagtttttgt agttgcaagg tatccgtctg cttcatattc tatgagatat
     2761 tcatgaccag gttgaagaat caggtaataa ttaccatcgg ttgaaccggt gcggtaaact
     2821 ccctgtactg tattgttggt tttatcgttg acacgtatta ccagatcttt tggaacctga
     2881 ttgttgatgt cggctacctg cccgttatac actaccattg tattgatgta cactccggtt
     2941 tgtgttattt cgtaaatgtc gaggtccccg taggtatcat ctttcgatga ggcaagatat
     3001 gctcttctgc cgtcaaggtt aacaacatac gaaacctcat catcaggtgt gttgataggg
     3061 tatccgatat tttcaggttt cgaaaactta ttgtcaccct caatagtggt tctgaaaaca
     3121 tcgtatccgc ccatcgattc atgccctttt gagctgaaaa acatggttat tccatcaggg
     3181 tgaatgaagg gggtctcctc atcataaatt gtatttacat cggtactgag gcactgcgga
     3241 gggctccagc tgccatctgg taaaagattc attttatata tatcgcgacc gcccactcca
     3301 ccctcacggt tgctaacaaa ataaagcgta ttaccgtcag gactgagaca agcatgtgtt
     3361 tccctgaatt ttgtattgat atcagcgctc agtaattttg ggtcagacca aagcaagtca
     3421 tcgcttgtca tagtcatcat tacattgctg taattggggc cttcgcttct gaaaatgaat
     3481 aaggtttttg catcggcaga aaacccaatt gaagcttcgt ttttatatgt atttacattg
     3541 tcttccagcc tcttgggttc tgtccaaaca aaatttttgt tttttgaaag gaaaatatcc
     3601 tcgtcctgat ttacacgttc ttctccagct cgtggtcttt tggatgtaaa aataaggtac
     3661 tcttcattca tgtcaattac cggggaatga tcgcaaaact ctgtattaat aatgctcccc
     3721 atattcttaa tgctagcatc tacagggtgg cttgtgaaat aaatggcatt gttggttatt
     3781 tcaatttccc ttttcgtttg ggcgactaag tcagcatcaa attttccttg cggagttagg
     3841 cgtgtgagaa gttcattgaa tgtggtcagc gcatcgttga atttataatt tactctatat
     3901 gcctgtccaa gatacaaaag gcattcaacc ggtgctttcc tttcacggtg gttatctgct
     3961 ttatagtctt tattaatgtt ttccgcacta aaaagaaggt attcaagcgc atttttttta
     4021 ttcaaccttg aattcaaata gcaaattcca attttatacc ggacatttgc attatctgga
     4081 tccatttcat ccagtttcag caacaacgaa agcgcttctt cgtaatttgc ttcatacata
     4141 taggcttctg catcataaaa taaattaaca tattttctgt caggcttgat ttcctgcgat
     4201 gtaacgctac aggtgaataa aacgccgatt aataaaatgt agattttctt catggttatt
     4261 gaagttttcg gttgatgaat tctaacttgt aaatataatg ttttaaaaat aaaattctat
     4321 tttctattca gttttattac ttcttcaaca attgattgaa tatcataatg gcatagacta
     4381 tataaatcgg actgattccc atgctcgacg aaaatgtcgg gaatgccaag gcgggccact
     4441 ttaacatcaa ctccattatc ggcaatgtat tccagaatgc tgcttccaaa gcctcctatt
     4501 aaggcagcat cctcaacggt gagaattttt ttaaatttct taaacaccga tgcgagcaat
     4561 tctgtatcga gtggctttac aaagcgaaga tcatagtggg cggtgctgat gccttgttct
     4621 gccagtcgtt tgcaggcctc ggttacctta attccgatag atccgaaact tagtatggca
     4681 atgtcgtttc cttctctaat ggttcttccg gaaccaattg tgatttcctc aaaaggcttt
     4741 ttccaatcgt tgtaaatacc tctgccacga gggtaacgta tgcaaatggg aaaattgttt
     4801 ttttcgagtt gggcagaaaa catcatatgc ctcaattcta tttcatccat tggagcggca
     4861 atcaccatat tgggtataca ccgcaggtaa gatatatcga atgccccctg atgagttgca
     4921 ccgtcattgc ctaccaaacc tgccctgtcg aagcaaaata caacatgaag attttgcagg
     4981 gctacatcat gaattatctg gtcataggct ctttgtgcaa aagtactgta gatgttgcag
     5041 aaaggtatca tccctctaat tgcaaggccg gctgaaaatg taactgcatg ttgctcagca
     5101 atgccaacat cgaaagccct gtgaggcatt ttttccatca tcaaatttag cgaacagcct
     5161 gtgggcattg caggagtaat cccaaagatt tttgagttgg attcagctag ctctacaatg
     5221 gtttctccaa atacatcctg aaaacgcggt ggctttttat cgatattttt ttcctgtata
     5281 atggtacctg tagcgatatc aaactcgccc ggagcatgcc aaattgtctg atcaatttct
     5341 gcaggtacat aaccctttcc tttggtcgtg atgcaatgaa gtaatttagg accggaaagg
     5401 cctttcatgt cagttaaaat tttaacgaga taaaagacat cgtgaccatc cacaggacca
     5461 aaatacccaa ttttcagaga ctcaaataaa tttgaacttt tcattacaaa cgacttaatg
     5521 gcattatcga cacgctgggc aattccctga gcattgggtc caaatttgct aaccttgccc
     5581 agcattctcc atacatcgtt ttttaccctg ttgtacgttt tcgatgtcga aatatcggtg
     5641 aaatactcct gcaacgaacc tgtgcttggg tcaatggcaa ttttattatc gttcagaata
     5701 ataagaatat cggtattctg aatattcata ttattcaatc cttcgaaagc ctggccggcc
     5761 gtcatggctc catccccaat tactgcaatc actttccgcg attcccccaa atgcttagat
     5821 gctacagcca ttccgattgc agctgatatt gatgttgaag aatgaccaac cccaaacgcg
     5881 tcgtattcac tttctttcgg tgacggaaat ccgcttattc ctttcagttt cctgttagtg
     5941 tgaaaaactt ctttccggcc ggtgagtatt ttatgcccgt atgcctgatg tccaacatcc
     6001 catattagta aatctatcgg agtgttgtac acataatgta atgctaccgt taattccaca
     6061 acacccagag aagccccgag atgaccaggg ttacaggata ctgcatcaat gataaaactc
     6121 ctcaattcat cgcataatcc gggcaattct ttaatgtcga gcttgcggat atcttcggga
     6181 taatttattt tatctaatac acccattcac aaggaaacca ttcaatttga agttcaaagt
     6241 tatataaatt tgattataaa tttcatcaga agattaacaa tctgttgtag ataaatattt
     6301 tgacagagtt tataaatgcc gcttcaatag tgtatttttg tttgttaaaa caataataat
     6361 atgtcgaaaa atgtgttccc attgattgcg gtagctgtaa tgattgctgc agtcgtttcg
     6421 tgtgttcctg cccggaaatt cgaagaagta aattccctga aaaagcaatg tgagcaggaa
     6481 agaagtattc ttaaaggcga taacgaaaag cttacaaccg aaaacaatga attgaaaaac
     6541 gagatggagc ggataaaaat taccatgtcg gcgcttgagc gcgacactgc aattaaaggg
     6601 aatgcatacc gaaccctaac gattcaatat gataaaatca accagttgta cgatcagctt
     6661 ctcgaaaacc agtcgaagct gcgtgagggt gccgatgctg aaactcagaa agcccttgca
     6721 atgttgcagc aaacaagaga tgaactgcaa aagaaagaag atgagctgaa gctattggaa
     6781 gcacaattga ataaagaaag ggcaaatcta gaggccctta aggcacagct cgatttaaaa
     6841 gaaaaggaaa ttgatgaaaa aaatgccaaa gtaaaagaac tgcaggcaat tctggccagt
     6901 caggattctg ccatgaatgc acttagagac aaagttgcca cagcactaag gggatttgaa
     6961 ggagaagggc tgactgtaac catgaaagac gggaaaattt atgtttctct cgatgaaaaa
     7021 ttgcttttta aatcagggaa atgggatgtg gacccaaagg gtgaagctgc tcttatagag
     7081 ttagccaagg ttcttgaaaa aaatccggat atcaacataa tgattgaagg acatactgat
     7141 gagcttgctt atcacccaaa cggtaacatc gaagacaatt gggaccttag tgtaaaacgc
     7201 gctacggcca ttgtaaaaat tctattaaag aactcaaaaa tagaccctgt acgcctaact
     7261 gcgtcaggca gaggacagta tttacctgtt gataaagccc aaaccccgga agcacgggca
     7321 aaaaaccgaa gaacagaaat aatactgact cctaagttgg acgaattata taaaatgttt
     7381 aacgagtaat tgaaaaaacg acacggacgt aaaatcaccc gctcaagggt gatttttttt
     7441 attccgaatt gtaatatctt tgaaattgat aagaagagtg agaaatccga ttcaatattg
     7501 ccggtacttt tctgcgagaa aaattataaa cctcagtaaa gccggtttat ctttcgcagt
     7561 aaatcgaatt tttcgaaagc atccctcttt tcgtaatcac tatgctgtta gcattgaaac
     7621 aacagcagta tgcaatctgc aatgtcctgc ctgcacactg gggtcaggag aattgttgcg
     7681 ggataaccgt tatatggatt atgaagtatt taaaagaatt gttgatgagc tgaggccaaa
     7741 cataattaat gcgaatttgt attttcaggg tgagccactt atgcatccgg aaattgagaa
     7801 tttaatcagt tatgccgaaa agaacagaat tcacacaacc atatcaacaa atggcggatt
     7861 gctatcgctc aaggcctcca gaattgccag tgccgggttg tcgcgactga ttgtctcggt
     7921 tgatggcatt actcccgata catacgagag ataccgtgtt aatggtgatt tttcggtatt
     7981 gtgcgagggg ttaaattcac tgatgaacct gaaaaaaacc tataaaaaat tcacaacggt
     8041 taccgaggct cagtttatcg ttatgcgaca taatgaacat gagattcatg atttttttat
     8101 ttgggcaaaa aaaatcggat tcgacagagc acacttgaaa tcgctgcagg ttaaccgtca
     8161 caaagatgtt gaactcgtac ctgcaaaatc ttgttatgca cgatacaaag ttgacgaaag
     8221 cggcaatttg cagataaaaa gcggattaaa aaatgcatgt tatcggttat tgtcaacagt
     8281 tgtaattaca gttgacggcg atgttgttcc ctgttgcttc gacaaaaatg caaaatattc
     8341 ttttggaaat ataaatcaac gtagcatcaa cgaaattctt gcatcagaga aggcgaaaga
     8401 ttttagaaaa aaagtattta atgacagaaa atcggttgat atttgtttga attgtacaga
     8461 aggtctaaac atcagatatt agaaattaag catgcaaaga gatgttatat accatacatt
     8521 gtttctatcg gtgggaggta atgttggcaa tcgacttcat tacatgagaa tggtgtacct
     8581 tgagataaac agcagcattg gtacgataat taacgcgtct ccggtttatc tgtcggaacc
     8641 ctggggtttt atgcatagaa ggtactttac caacgcagtt tttcatgtgg aaactgcgtt
     8701 agaactctgt gaaatattac ctaaactgca taagatagaa gcaaacctgg gcagaataag
     8761 aagtgctgct acagggtata tcgcaagaac aatggacata gatataatcg caatggacga
     8821 tactgtatat aatgaagaga acgttactct tcctcacccc agaatgagag ataggctttt
     8881 tgtgcttttg ccattgtttg atattgcacc ccaatgggtg catccacgca caggagagaa
     8941 tattcaggaa ctgatacgta aatgttcgga tacaggtaaa atcagaaaga caatgctttg
     9001 ttattatgac tgaagaaatc tacatgaaaa tggcttatga tgaggccatg aaagcttacg
     9061 acgaaggaga ggttcccatt ggggcggttg ttgtttgtga aaataaaatt attgcccgct
     9121 gccataatca gacagaaatg cttcacgatg ttacggcaca cgctgaaatg ctggctatta
     9181 cagccgcaga aaattatatt aatggcaagt accttgataa atgtacgcta tacgtaacgc
     9241 ttgagccctg cgttatgtgt gcaggggcat tgtcgtggag ccagattgga agcattgtgt
     9301 atgcatgtag tgacgaaaaa cgcgggtatt ctcagttttc cggtaacatt ttgcatccca
     9361 aaaccgtggt tagaagtggt gtgctggaag aaatatgtcg taatttacta ctggaattct
     9421 tccgggaaaa acgtaaatta taatattcgt agcgaaacca gatcaatttt tgatgcagat
     9481 acttttaaaa tcttaacaat aagcttttgt ctgttgtcga ttatggtaat ttcgttgtgt
     9541 atctgaggaa aacttccgtg aaaatacata atgtaaccgg ccagagtttt atactccgac
     9601 gactccgtaa gatttagccc gtatttgttg tttaggtcct ctgtttcgag gcgggccgaa
     9661 aatatatatt cattttccga aacctgcttt tcaatcagtt ccgatacatc gtattcatct
     9721 tcaatttcgc cgaatatttc ttccatgata tcttcgatgg ttacaatacc tgatgttccc
     9781 ccgaattcat caaccaccac ggcgatactt ttgttgttgc ttataaagtt ctcaagtaag
     9841 cgataagcag gcattgtttc gggaacaata agcactgggt tcagcatatt tctaactttc
     9901 tgagcgtttc tgaataagtc gagcacatga acataaccgg ttatttcatc gatactttct
     9961 ttgtaaatca aaatttttga atacccgctt tcaataaaaa tattttttag ttcatcagga
    10021 ttcccatcaa tctgaaaagc acatatttcc gtgcggggaa ccatgcagtc gcgcagcttt
    10081 atatcagaaa agtcaagagc attctggaat attttcagat cgcggttttg tgtttcagta
    10141 ttactgtaag ggtcttctcc aatttctgac aatgtttgat tgatatcaat tttcccaaaa
    10201 acacgctctt cgtcagcttt atgtatttta acgcccgtta aaacccggat aatcaaacct
    10261 gacagcccga caaagagtat tacaacaggg tataatacgt aatagattat taataatgga
    10321 aatgcgaaga acttcaatgc atcgttgctg gtgttacgaa acatcatttt tggcagaaac
    10381 tctcccaaca aaagaattac cattgtagcg cacagggttt gggtaatcag aattgcaaat
    10441 gcagaatctg ttatgttcag taaaaagggt tcaatgagtt ttgccgcata aatactatac
    10501 accgacagcg caatattgtt tccgacaagc attgtcgaaa tatactgaga aggattatca
    10561 gcaaaaaatg aaataatttt aggactaaaa ccaccttgtt tcttatcgag ttcaatccgg
    10621 agtttattcg aagacatgaa agcaatttcg agccccgaaa acaaagcgga aaaaagaaca
    10681 gcaataacaa ttataatgat tgtgttcatt tcataaatct gttctgtcgc ttttctaacc
    10741 gcttgcggct gctccttctc agaaaataca taccaaccga taccgccgac aatacgtaaa
    10801 acagccatgt aaactggaag cctatcttac ttgtttgcaa tatacctaca atcaggcaaa
    10861 aaatcgacat accaagccac agaaattcga ttataacaga aagtgtgatt ttattcttca
    10921 tcgttaatag tgattgttcc tttaggtttt ataattttcc agctgctgaa atcctgattc
    10981 gaccgaaatc catcgccgta aataatttca tcggcagtgg ttattcttac aaatttatcg
    11041 gaataaattt cttcgaggtt catatcccac cataatagct cggtattaat cttttcatct
    11101 ttttcaaggt tcatggcttc cacattggtg cgcacttccc aaaggttttt atcgggaaaa
    11161 taccgcgcat actgacagga aagcttgcct acaacgttga ggtgactgcc ataaaactca
    11221 accagcaatc cttgtgggta ttcatcatat ttttccggtc ctgattcaaa atgacggtat
    11281 tctgcagctg tgagcttgag ttttaccacc gccgagtcgg agtaaatggt ttctatgttt
    11341 tttaccgttg tggctggaag agtagtaaca ctggtcagtt catttacctc ttcgatattg
    11401 ttggagcagg aacccaaaac cacacaaatt gcaataatgt ttgcaagata tgtggttttg
    11461 tatttcaagg tatgtaatgt tctggtttac tgaataaacc ttgctgttgt cgattcgttg
    11521 atccagcatc cgacagtgta gggttgacct tccgaaacat tgtaccagaa tgccttttcc
    11581 tgagttggga aataacctga atatttcctg ataagctcgt ttgcttcttc cgaaaccgat
    11641 gggtcagctg attttgcttt ttgaaactga tcaactacta cccaatacac agcaacttta
    11701 tcgagttcag tttctccgca tcctgaaccc cctgaagcat aacatttacc aataagtaaa
    11761 tatgccttac catagttggg gtctgatgct agtgccttac gggcgaaatc acgcgatttt
    11821 gcaaacattt tcagattgcc gaaatataat gtggccaatt cgtagtatag gtcggctttt
    11881 tgcttttcgt cgctttgcag acgaatggct tcgttatagt aaaactcagc tttttcggta
    11941 ttgtttttaa taaaatatac ttttgcaatt ttatgggcag attcggcatc cttttcaagg
    12001 acatacagcg actcagatac agaagcatac aagtcggttg taaggcaatc ctggtttttt
    12061 gttttttcga tgagtttagc aattttctga agtagaacca aatcagtttt gtttgcttcg
    12121 tatttcggtg taaaaatcgt tattatggct tcgcagtcgg cagcaggagt gtttacgaac
    12181 atctcttcta ctttttcgtt tattttactt agtctttcaa cagttttatc atctcctgca
    12241 gcagctgcaa tttgcttgtc gagaatttcg gaaacggttg agtagttttc aactaccgtt
    12301 tcttttgata gatcgccact tttcagcagg attaccgaaa cattcataaa atacccaagc
    12361 actgaacttt ccgatgaatt tcctgtaagt tcaatggaac gtttgtagat gttgtaagcc
    12421 tgcttaatac tgtcaggacg atatttcaac aggtcgttgg ctttgcggcc cagcactttc
    12481 ccttcttcat tatagtattt aattctcata tcgtaaacca tcatcagcgt atcgatatac
    12541 gattcctttg tgttctggtc tttcgcattc gatatcagat aatccagaat tgtaggacca
    12601 tgaatgtaga gattctttgt tgatgcaggg cagtttttaa tgcaccatgt ccaggccgga
    12661 taggcatcat gatagttttc ctgtttgaaa aattcggtgt aggtcgaaag ttttacctta
    12721 cactcatcgg ggttgctgcc aaaattagaa tcttcttgtg cgtttaatgt catgctgcct
    12781 aaagcggacc ccagcatgat cagaaaagct cttttaaaaa atacagtttt cattttcaat
    12841 caaatttacg tttaataaac caaacatccg acagggtaag gttgacaccc agatttgcgt
    12901 aggtttcacg aaccaagctg ttgcgtgttg tccctctctg tccgagctcg atcgaaatat
    12961 taaaagatgt cttagaacga ttaagcggta aaccaaatcc aaaacttatg ccaaaatctg
    13021 caatttgctc tccgtaggtg gcaaagttaa tataagtatt attatagaag gcaccaaaac
    13081 ggtatgcaac acgctgggaa tatttcgagg ttgcacggct tggtcctgca ggcaagtatt
    13141 cagcaccgaa cctaaagcag ttgctgttgc ccagagagtc ggactggtta aaaaatttcg
    13201 catccgacca gttctgcata ttgtaatcga aggcaagttt caatttatcg tcgaaattat
    13261 agagcaatcc accaccggca cttaaaggca tcgatacagt accccgctgg ttatcttcgt
    13321 tgtaaatagt atcgaccgta gaactgctgc caacgctcag ggaattggta accaatacat
    13381 ctctcctgct gttcaaattg ttggggtacg aaaacacagc accgagcgat aatttgcttt
    13441 tgcttccaag tgcaaagtcg tattgtaatc cggctgaaaa acagaagtcg ttaacatata
    13501 caatattctc ttcaagaaca ttcacatagg ctcctgaatc gtcgctgaaa ataacactgt
    13561 ttgaacggtt ctgcatacca aaaaggtaat aggcgtttac accaattcga aaatttctga
    13621 aaggtaaaaa ggcatttccc cagtacacct gattaatacc tccgctgcct tcgtaataat
    13681 aatacttggg tacattaaag gttgaatcgg aagagcgaat gctgtaccct acagaactaa
    13741 atggcataat gccgattgag gaagcccacc accttgtaac gggaaatccg aaagcgagat
    13801 acgataactt tgtatcgttg ctgagtgcac tcaggtcggg gttggtggtt ttgaaataac
    13861 gaattcccga ttcaaggcca aattcgaaga taaaacacat actatcaacg gaagcaatgc
    13921 tggctgggtt aacaacattc agattcctgc cagaggaaaa tgcagctgca gttcccccca
    13981 ttgctcttga aggaccaaag gaccgggagt ttaaatctcc caaaccgtat cgcgaataag
    14041 gcgaagaatt ttgcgcttca gccagatatg cggctgttat cagaaataaa acaaaaatta
    14101 acttattcag catttaaatc aagaatacgt tttaaaccta ttggtactaa ttcaaagttg
    14161 acaaaaatgg gactttttat tgaatttaca aaataactta tatccccgcc ggttaaaata
    14221 accatactgt caactgtttt attgtaccaa tcgcgtatat aattttcaat ttcgtaaagt
    14281 attccgttct gaacacctga aacaattgct tctgcggtgg aagttcctgt ttcctgccaa
    14341 gtttctacag gggccattag cgggagtttg ccggtataat tactcaggct tttatatcgc
    14401 aggatcaacc ccggagatat atttcctcca agatatgcag catttttatc aacataatcg
    14461 taagtgatgg ctgttccggc atcaatgata agaatatttt tcgcggggta taagaaattg
    14521 gcaccaacag ctgctgcaat tctgtcgccg ccaagagttg caggtgtctt atattgtatg
    14581 gaaaggggtg tgcacagttc tgcactcatc ataatcggta taacaccttt actgcgtagc
    14641 aggccggaaa gggcagcaga acgtgcggct acatcacaaa ttattgcacg gtcaggcaca
    14701 atgaaccccg aattcttcaa ttttatttct tcccctgcag agatgtcaac tgaggaaacg
    14761 atagccccgt gattattgaa gtaacgaagt ttggtcttgg tatttcctat atctgcaact
    14821 aaatccatgc ggcaggcaat ttacaaaatc tagcttactt ttattttaaa ccgatacata
    14881 ttattatcaa aattattata ttcgcagcct gaaatttatt gaaagtaaaa aataaaattt
    14941 atcaataatg gcaattttac aaaaaatcag aaacagagca ggaatttttg taattatatt
    15001 tgttggtgtt gcattgttct tgtttataat tgaccctaca acctttaatt ctttgtttca
    15061 taaaaacccc actgatattg caagtatcaa tggtcatgat attaagtatg aagaatatct
    15121 gaagattgct caggagcacg aagattttct gaaggtatcg cagcaaaccg acaacctaag
    15181 ccctgaaaca ctggaaagcg ttcagcagca ggcatggatg gacttgctcc gcatgtatat
    15241 cctggatgta aatttcgaag acttgggtat tacagtaagc gacgaagagc tcgaagatat
    15301 gctatgggga aagcatctta gcccgattat tcagcaaaac tttaccaacc cacaaacagg
    15361 ccttatcgat acgtcgatga ttaaaaatta ttttcagaat gccgaaaagg atgaaaccgg
    15421 aaagcagctg tttatcgcag actatttaaa aaaagccatg gtgtatgaat atcagtacgg
    15481 gcgtttacat attaaatatc gtactcttat tgaaaaaggc ttgtatgtgc caaccttcat
    15541 tgccaaagac gattattaca gtaaaaacaa caaggctgac atcctggttg ttgcacgtaa
    15601 ttacaaggat gtggatgatg cagttattaa agtgagtgat gccgacatta aaaattatta
    15661 tgatgagcat atcgaaagat ttcaaatatt ggaagccaac agagatatcg acttcgtaat
    15721 ttttgatgtt ttcccttcga aagaggacac cttagctgct ttgaaagata ttaaaaccaa
    15781 ttccaaacag ttttgtacca ttgaagcgga aactgttacc gattttctga acagctactc
    15841 cgattcactt tatcaggaaa aatactatag gcctgaagaa tttgcttttg ttggagggtt
    15901 tgatcctgca acgatgaaaa ctggtgacac atcggagatt tttcatttcc agggatcgtt
    15961 tattcaggct cgcgttctcg atgtttcaac ccgacctgat tcagtaaagg caagctatat
    16021 gtttattatg gccgatacaa ccaaaagcat tgagcaggtg aaggtacttg ccgacagtat
    16081 ttataatgtg ctcgtaaaag gggctgattt tgcacccctt gcaatgcagt attccgatca
    16141 cccagcttca aaagaaaaag ggggagattt gggatggtta accgaatcga tgcttggtga
    16201 cgataaagaa cttaaggatg caaccttcct gtcgaaaaag ggtgaaatta aaaagataga
    16261 aaccgaattt ggcgttcata tcattaaaat tgatgagcag actatgttgt tgcaaaagtt
    16321 ctcaatagca tatttataca agaacatcaa atatagtaca cagacagcgc aaatgcgatt
    16381 ctctcaggct agcgaatttg catctaaaaa caataccgct gccaaattcg ataaagcttg
    16441 tatcgacctg caacttacaa aacggatggg gaatgccatt aaagaaaacg atatgaaaat
    16501 ccccgggttg gtgaatccaa gaagtattat taaatgggct tacggtgagg aaattgaaaa
    16561 aggcgctgtt tctcctgtat ttgaacttaa cgatatgtat gtggttgcaa aagtcgctac
    16621 aatcagagaa aagggcactg ctgccatcga agatgtaaaa gaacagattg agcccattgt
    16681 tgttaagaag aagaaggctg ctttgctcac cgaggaattc aataaggacc tgagcaaagg
    16741 agaatcgctt gaagctatcg ctttaaaata caacaagaag cttgacacta ttagcaatat
    16801 tacatttaac tcgatgtcat tgcccggtta tgggttggaa cctaatgtta ctggaactgc
    16861 cagtgtactc gacaaaggta aaacatcgaa accaatcgaa ggcaacaatg cagtgtatgt
    16921 aattgctgtt gttaataaag ttccggcacc agcaaaaact gatttcaagg acgaacagct
    16981 ttatctgatg cggtcagctg cttcaaaaac ctatcaggta tttgaagttc ttaaaaaaat
    17041 tgctgacatc aaagactatc ggtcaacctg gatgtaaaac tgattttaga acatgttttt
    17101 atagggcgac aaatttgatt tgttgccctt tttcgtaagt tttttcggag acttgtaaag
    17161 atagttttta cctttacttt tattttatct acaatgaaaa cgttttttac agcatgcgtc
    17221 tttgtatgga tatgttcttt gggactatct gcattacctg tttctgttga aaatgctaca
    17281 aaaggagctc tgcattttat ttcctgcgaa aagaaactca atcattgtca gattgtagat
    17341 tttcagcata ttactgtagg tggtgaaacc gttgcctatg cttttaacct tagccctatg
    17401 ggttatatcg ttatgagttc gagttactat ctacccccgg ttatagcctt ttcgacagaa
    17461 agcggtttcg gggaattggg agaacacaat cctttataca cgctaattgt tagtgacctc
    17521 cccctgagaa tgaagtattc atctgatatc gaggccggct acgccagaga aaatatatta
    17581 caatgggagg cattgctgaa tcaaaaccct aggagtaaag gtgaacccca gcaatggccg
    17641 gaagggcagg aaggatggat acaatcaagc tggaaccaga atgctcccta ctggaatatg
    17701 tgtccggttg acccagtaac ccatgtaaga tgttacactg gttgccctgc aacagctatg
    17761 gctatgatta tggattttca tgcaacaaca aacaatgttc aattcacaga tgaagatgat
    17821 tattaccaca gttatgccgg gcgaaattac tggattgatg atgattttga ggcaaacggg
    17881 ttcccatcgt ttcctgatct taacaaatac cttgataccc tgaatatgca ttaccagcta
    17941 aaaagcgtta ttacgagcaa cgacaaagcc gcattaagtt ttgcatgcgg catagctgca
    18001 caccaggtat acacatctca gggctcaggg acttttgccg tatctcaggc ctatgatgca
    18061 tatatgcgtt ttggttgcat aaccgcagtg cttatgtacc cggaggaaga aggagttttc
    18121 gacagcatca ttctgaatat acaacatgct ttacccgtgc accttgcagt tgtcgatgag
    18181 agtaattcat cgggacataa tcttgtaata gacgggtatc gtaccgataa ttactttcat
    18241 ctcgactatg gctatggaga gtattataac gggtggtacc tgatcccttc cgaaatacag
    18301 atgggcctta cagtaattga gggtgtcatc gtaaatatta tgaaacaaaa cacttcggga
    18361 atatatttcc caattgctga gcaaagcaat attgaggtat ttcctgtgcc tgctaacgat
    18421 ttctgtaaca ttcaatgctg taaactgtcg gacgggaatg ccaaaattgc agtatgtaat
    18481 ggctttggtg ctgaaatttt aaaaacttca gttgatatgt tcgattcgga aaatcggctt
    18541 agaatcgaca caggctcatt acccgaagga atgtatttcg taaaaataaa ttcaggcgca
    18601 gaaacaaaaa ccgctaaaat tattgttata cattaaatat cagggaaaaa aatatgattt
    18661 tttagaaact gttttgaatt ttttatttta atctattaag cattaatttg gtcattgc
//
LOCUS       DAOOQP010000053        18590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3633_length_18590_cov_6.225303, whole genome shotgun sequence.
ACCESSION   DAOOQP010000053 DAOOQP010000000
VERSION     DAOOQP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_18590_cov_6.225303"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLK75_07045"
     CDS             <1..641
                     /locus_tag="PLK75_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY39040.1"
                     /translation="LSFTIPDPPPVEKGILLDLGGAGSGTPNPGPSSPSSSDAQHNAS
                     SQHTPVSGSLTQDLEEAPALPSGNNPDNQQLTPEEIQRREKANKISNLTQFGNGSPGN
                     GVGPGSGLPGSNGDGSTPGDGGGPGGVGGNVGSRKISKIVEPMQKPDMFGKVILSYLV
                     NEQGVVSNISVVSSNCAECTSYAITALSQWKYEPLPGAKLQKGQQTFNFSPK"
     gene            complement(668..1714)
                     /locus_tag="PLK75_07050"
     CDS             complement(668..1714)
                     /locus_tag="PLK75_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY39041.1"
                     /translation="MKYYSSNRLAFLFSVLLSVVIMLIAMVFNLYYYHSFNMVFCVIL
                     LSVSFLISFFSLRFIFFQFIYDRIRVIYKAIHKRKEAGRNFNAKFNNRADMIHQVEAD
                     VNEWDREHNQELEQLRQMATYRKEFIGNVSHELKTPIFNIQGYTSTLLEGGLEDENIN
                     RKYLSKIEKNINKMISIVNDLEAISKLESGRIKPDFKVFEPEKLIAEVIESFEDRANE
                     KKIALSFKKHYEKMKSIEADRHLINQVLANLILNGINYAKEGGFVKVELFDMDKHLLI
                     EVTDNGIGIPDKDLPRVFERFYRVDKSHSSNTGGSGLGLAIVKHIIDAHGQKVNIRST
                     EGIGTTVAFTMKKV"
     gene            complement(1739..2455)
                     /locus_tag="PLK75_07055"
     CDS             complement(1739..2455)
                     /locus_tag="PLK75_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY39042.1"
                     /translation="MNNPQKILVVDDDPDILEFLSYNLDKEGFIVEIAGNGKDAIDLA
                     GKYNPDVILLDIMMPEMDGVEVCHILRSSTQFDHTLIVFLTARSEDYSQIAGFDAGAD
                     DYITKPVRPKVLIKKIQSMLRRSNRLSNRRSDVDEAETVLRFNGLTIDVEMHSVMKNG
                     AEVILPKKEFKLLKLLVSKPGKVFSRDEIYSAIWGDEVIVGERAIDVHVRKLREKIGD
                     EFIRTFKGTGYKFEYRPDEQ"
     gene            complement(2467..4398)
                     /locus_tag="PLK75_07060"
     CDS             complement(2467..4398)
                     /locus_tag="PLK75_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39043.1"
                     /translation="MRSKGGFILNIVSAAVLIALFFSCTSEPSAKKLSFSVDNVHWLT
                     NYQPGQDNKQLMLDYREAILGFVIPSIRQTESGYFNYEFTIKNTSSRKEKYYYKIYYQ
                     NETYKVPEVSDNSNQMHPYASENFYGSWEDTLLGFKPVGEIEPGEKKQIQDFFRIIGN
                     PRDEKNCYFNGVNDRWKRNPRVGDYSILLVIATEVDIKKIPDYLQHINKTYKNDFVNP
                     YYYFLYGDGAKLGDSFIKKYEAVLNVKAKPDLGAGIYAHDYDFRKTLNYSETFTCNCN
                     NSRTIYENAAVQQFIHYVDRSSKMFNIPVITDVLNDGYSKEEYNWNNAFTRKEELIGI
                     TPTNAKYPCLTVSSDSVENKIVIQNPGATYGDWRKENVGIITRHGFTYGKITVKANLT
                     ELLNKDNVWNGITNAIWLIYQGGFGEESGWNLRRSCEKNGYMATYWGGTEEKRVNRVA
                     YSEIDFEILKTVPYCPDNEYPPLYNEPVVNQKNPADWNVLMPEYIASLDNTIAVCCTN
                     WDMACASPSNFGTGCNEIQYQGRKFHWFRWEDTYRAVIEKYFISDDEVFGRDYYYFQI
                     DWQPDKIIWRIGPEKDKLFVVGFVDESISMIPNNQMLLIITQEFHNTQWWPGSPYLQE
                     NIPFPKNAITGEIYEITIE"
     gene            complement(4388..6577)
                     /locus_tag="PLK75_07065"
     CDS             complement(4388..6577)
                     /locus_tag="PLK75_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39044.1"
                     /translation="MTFPYLKKNKNLYLVFTFIAILLLSVLLYIAAHWEFKNTDGKKN
                     NLNSEVIPEKFIINFETDYLILENSKGGISTEHALSGKHSTVMAERNAYSAAIVFDLD
                     NIASANTGSVKIGAWFLPVKTKMNVLLVLSVFDEDMQTQLYWDALQVNRDNVTAGEWF
                     YASRQFTIPDSLMNQGNKVRIYGCNYLGDGHIVYIDDIEISFQNGDAQTRLKRSKLID
                     YEGISGDGITTRVAYNSQKSASAAGKNTYTAPTMIPLADFDLTQVDRVNFRFYYLSEK
                     AQMDYVLVFAVKDKNNNDVFWYGYLLGKTNSPVKKWIKEQDSFEIPPEVCSPGNTLCI
                     YGWNRNNNSVYVDDILVIMKNTDDIKLGNNALCDLTVDSVFKPAINTPPYPCSYGTYV
                     MGKVLPEKGIIKKYNCISNIRLLNQQHEQVVAGNSPNTLSIFYAVKEGLFSKPVIFSE
                     KVNENALMIPIDWDNNKKDEICFIDNSGRKIICFSLNNDAYQISGTKLWEKQFESFDG
                     FSTTTWGACISTTGANKPAILVLQGTKFGCIYPSEKREPVFITEKNNFPSISKFYCYD
                     VLPEIAGSEIFAIYGEETKMVYRIFSVSLQKGAIRITQVAQTPEILEDHYLLNARADY
                     ISIGSHKNNSGKVLVLDRSWRFDLKQINVDKKMYTIEKSIDFKGFPDGLNPKYYTNNS
                     IVCGNYTGDETPEIMCFSFIDVSSANLTQSTTHDKEMMKSKVEIYAF"
     gene            complement(6549..7529)
                     /locus_tag="PLK75_07070"
     CDS             complement(6549..7529)
                     /locus_tag="PLK75_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY39045.1"
                     /translation="MKELHDINLSVIIPVLNEEKNIGNLYTEIIAVLQSNSIESYEII
                     FVNDGSSDLSLPVIKGLSVSDNKVKYIDLSRNFGHQIAITAGLEVCSGKRIVFMDADF
                     QDPPAVLGELYNKMNEGYDVVYAKRESRKGDSAFKKLTARFFYRILSILTRSRIPVDT
                     GDFRIISRRVANLLKKMPEQQKFLRGQIAWIGLKQTSVAFHRDKRLDGVSGYSFKKMW
                     RFAVDGITSFSDFPLKFATYMGFVFSLVSFFIMLWALYQRIVAKEYVQGWASLIISVL
                     FIGGIQLISLGIIGEYISRIGNNVRNRPLYVINETNIEPNKDDVPVSEKE"
     gene            complement(7568..9040)
                     /locus_tag="PLK75_07075"
     CDS             complement(7568..9040)
                     /locus_tag="PLK75_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY39046.1"
                     /translation="MKKNFLINLAFLLLLNLLVKPFWIFGIERSVQNVVGAQEYGLYF
                     SLFNFSLILQILLDLGITNNNNRNIARHSHMLSKYFSNIVSLRLLLAVFYALVSLVAA
                     LALGYDIRQFSVLGVLIVNQFLASFVLYLRSNVSGLQFFKTDSILSVLDRFIMILICG
                     ILLWGHVTSVPFRIEWFVYSQTAAYLITAIVAFIIVVRKTTYFKLHFDYRFYAVYIKQ
                     SFPFALLILLMAFYNRVDSIMIERLLPNGQEESGIYAQAFRILDAFSMIAYLFSVLLM
                     PMFARMLQKKENVGGIIKIAALLLIVPAIVLASVCIVFNNELMHLMYVGFAERSAPVL
                     SYLMVGFIGICGTYIFGSLLTANGSLKYLNIMAAAGMVFNISINFILIPAFWSTGAAF
                     TSMVTQLITMTAQIFIAVKLLNIRANYSLIARIILFFPISIGISFACRYLFHGWFTAF
                     IVAVALSLLFAFLIRIFNLRAIGELIRPHIVTENDNETSE"
     gene            9238..11388
                     /locus_tag="PLK75_07080"
     CDS             9238..11388
                     /locus_tag="PLK75_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOY39047.1"
                     /translation="MKTYQTNQIRNVALLGNGGSGKTMLAESMLFLGGIITRKGDIDS
                     KNTVSDYRILEQTNGNSIFSTVMYTEYKDTKINIIDNPGLDDFIGQVVSSLNPADLGL
                     LVVNAQNGVEVGTEIHNRYLEKAHKPVIIAMNHLDHDKTNFEKSIDMLHESMGGNVVV
                     AQYPVNQGVSFDSIIDVITMKMYTLKDGKTIISDIPASQMSHAEGLRMQLIEKAAEAD
                     EALMEKFFENDTLSEAEMMQGIAAGLPHRGIFPVFCVNARNDLGVERLMDFLAGVAPA
                     PDHCEPINNSEGEPVKCCTKEKPAIFVFKTSIEEHIGEINYFKVMSGEITESIDLYNS
                     TKGVKERLSQLFVCAGKNRTKVPKLVAGDIGATVKLKATKTCHTLVAPGFDWTIPSIE
                     WPSPKFRTAIKTVNEGEEEKLGEALNRMRDEDQTIVFEHSKELRQLIISGQGEYHLSI
                     VKWHLDNQFKIETNFLKPRIPYRETITKKAQADYRHKKQSGGAGQFGEVHLIVEPYSE
                     GLPEPDMYKINNKEYKISVRGKDEIELEWGGKLQYFNCIVGGSIDARFMPAILKGIME
                     KIEVGPLTGSYARDIRVCVYDGKMHPVDSNEISFRLAGRNAFSMAFKDAGPKIMEPIY
                     DVEVFVPGDRMGDVMSDLQGRRAIIQGMSSEKGFEKIMVRVPLAEMGNYSTALRSLTG
                     GRAMYNMKFSEYQQVPPDVQEALLKEYAQQDSEE"
     gene            complement(11489..12343)
                     /locus_tag="PLK75_07085"
     CDS             complement(11489..12343)
                     /locus_tag="PLK75_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOY39048.1"
                     /translation="MNKKVSVARLSVASNTLLIALKLTVGLLSGSVSIISEAIHSLMD
                     LVAAVIAFFAVRVSDIPADERHPYGHGKFENVSGVIEALLIVGAAVWIVVEAIHKIEN
                     PGEPEFVLLGATVMLVSAVVNYFVSRKLYKVAKETDSVALEADALHLKTDVYTSLGVG
                     LGLLLLWITGIPLIDPIIAILVALLILWESFQLLKKAFTPLLDVSLDESEVSRINEIL
                     AARNLKYHDLKTRKAGNYRFVEFHLELPAGMVLAEVHRHCDDIEEILKTQLEHLTVTI
                     HVEPVGKA"
     gene            12573..13073
                     /locus_tag="PLK75_07090"
     CDS             12573..13073
                     /locus_tag="PLK75_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOY39049.1"
                     /translation="MKTLIIYDSFFGNTEKIAFAIAKSLTNDHSVEIHKASDFSDFIN
                     SEFDILILGSPTRGFKATPLVQAFIDDYSLKPGKPMAVAVFDTRIKIENIKQGFFRYI
                     VKKGGFAADKMMRAFSKKQIDTIVAPEGFYVIDKEGPLEAGEEQRAENWAQTILENYT
                     RNTNLN"
     gene            complement(13180..14058)
                     /gene="fabD"
                     /locus_tag="PLK75_07095"
     CDS             complement(13180..14058)
                     /gene="fabD"
                     /locus_tag="PLK75_07095"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOY39050.1"
                     /translation="MKAYVFPGQGAQFPGMGKDLFGNSSTIAALFHKANEIVGYNLTE
                     IMFQGTPEELKQTKVTQPAIFTHSVLLAIANGNNFTPDMVAGHSLGEFSALTAAGALS
                     FEDGLSLVLKRALAMQKACEAAASTMAAVLNADDALIAEICQSVDDIVVPANFNSPGQ
                     VVISGTIRGIDLAIEKLTAAGVRKVIKLPVGGAFHSPVMEPAREELAAAIENTKFAAP
                     RCPVYQNVDAKPHTNPVEIKQNLILQLTSPVLWTQTVKNMVADGADMFIESGPGTVLQ
                     GLVKKIAPEVNTMSIA"
     gene            complement(14337..14963)
                     /gene="folE"
                     /locus_tag="PLK75_07100"
     CDS             complement(14337..14963)
                     /gene="folE"
                     /locus_tag="PLK75_07100"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOY39051.1"
                     /translation="MEQSLNNKKKGGYVKTEKYDAEKIQQIGGHYREIISLLGEDPTR
                     EGLEKTPLRVAKAMQFMLQGYNMDPEAILNSAKFHEDYHQMVIVKDIEIYSLCEHHLV
                     PFFGKAHVAYIPNHYITGLSKIARVVEAYSRRLQVQERLTTQIKECIQNTLNPLGVAV
                     VIEARHLCMQMRGVQKQNSVTTTSDFVGAFCKEATRKEFLHLISSKLS"
     gene            15013..15669
                     /locus_tag="PLK75_07105"
     CDS             15013..15669
                     /locus_tag="PLK75_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39052.1"
                     /translation="MKVLIVSATPFEAQLPDSLFQVFTAKGYNPNDGRIPVLVSGPGI
                     MSCVFSLAETLQHYHPDIIINMGIAGSLKSAIKIGTHCVVASDYLIDSGIFRNNVFTH
                     LRDCDFSLNNDKLFRATADILPLMPECSAVHGQTVGLFYDLPLHQRPEPYQQSAADIE
                     TMEGAAVFYVAASKGIPVCSIRTISNTVGIAHEPGQFLNAVTSNCAIIETLISRLGAS
                     "
     gene            15635..16477
                     /locus_tag="PLK75_07110"
     CDS             15635..16477
                     /locus_tag="PLK75_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOY39053.1"
                     /translation="MKPSFHDSVHLNFAISPCPNDTYIFHALKNIQGFAPGIEPVFTF
                     ADVEALNAMALSSEPDVCKLSFHAYAHCHNNYRLLGSGSALGHACGPLLISKRKIYPD
                     ELRDVHIAIPGKLTTANLLLGIAFPEATRKTEYVFSDIETVVLDGECDAGLIIHENRF
                     TYEKKGLKKIIDLGEWWENKFQLPIPLGGIAIKRSIAPHIAENVEQAIRNSIVYSHTH
                     QSEALAFCRSLSQEMDEKVLQQHINLYVNDYSLDLGEKGRQAINFLFARAIETKIIPN
                     GVEL"
     gene            complement(16587..17273)
                     /locus_tag="PLK75_07115"
     CDS             complement(16587..17273)
                     /locus_tag="PLK75_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39054.1"
                     /translation="MKRVFLSTIACVIAVFTIAQTCPDYYPLHEGASWETQNFNAKDK
                     LESTATTKVLAVNNTAVGYEATIETNSIDAKKNQFTSKMTYKCENGVIYVDMSNFMDQ
                     STMDAYKDMDVKVENVGMQYPSTLVPGTTLPDAKFTMSASSQGFQVFNMVVNITDRKV
                     VSSESITVPAGTFQATKITYTVTSKVAFMNTQASVTEWISSSAGVVRSENYDKNGKLM
                     GYMVLSKLVK"
     gene            17467..18258
                     /locus_tag="PLK75_07120"
     CDS             17467..18258
                     /locus_tag="PLK75_07120"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOY39055.1"
                     /translation="MNKRIVIIGAGNVANNLASLLQGSAYTIVQVFSRSNNSAAALAQ
                     QCACSHTCNFGKITNAADIYIFCLSDDANMQYIPQFRHTAKLLIHTSGSMSPAVFAGL
                     TANYGVLYPLQSLKKGISIAASQIPFLIEASNQESFKQIAAIAEAMGATFTHADTIHR
                     TWAHISAVFACNFSNHMLAIASDIARKNNVPWEMLQPLIVNTFNRSLHADPFTVQTGP
                     AYRNEKSVMQKHSEMLHDGFNTYENLYKTISNSIFEMYKTNKKED"
     gene            18258..>18590
                     /locus_tag="PLK75_07125"
     CDS             18258..>18590
                     /locus_tag="PLK75_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOY39056.1"
                     /translation="MIPDESLNFKQLLKNIKAIVLDVDGVLSSCQVLIEPDGELQRNT
                     NVKDGFVVQLATRMGIRIGIITGGKSDAVVKRYNKIGVDDVYYQVKDKNTALDEFMNK
                     HNLKPSEIL"
BASE COUNT         5551 a         4005 c         3612 g         5422 t
ORIGIN      
        1 tattaagttt taccatacct gatcctcctc cggtggagaa aggaatattg cttgatcttg
       61 ggggtgctgg cagtggaact cctaatccgg gaccatcttc gccctcctct tctgacgcac
      121 aacacaacgc ctcctcacaa cacacacctg tttcgggcag tttgactcag gatttagaag
      181 aagcgccagc cctaccctca ggcaataatc cggataacca acagcttacc cctgaagaaa
      241 ttcaaagaag ggagaaagca aacaaaatct cgaacctgac tcaattcggg aatgggtcgc
      301 ccgggaatgg tgtcgggcct ggaagcggat tgccaggttc aaacggagac gggtcaaccc
      361 ctggtgatgg tggtgggcct ggtggagttg gaggaaatgt aggttcacga aaaatcagta
      421 aaatagttga accgatgcag aaacccgata tgtttgggaa ggtaatcctt tcatatcttg
      481 ttaacgaaca aggggtagtg tcgaatatct cggtggtttc ttcgaattgt gcagaatgta
      541 cgagctatgc tattacagct ctcagccaat ggaaatatga accattacct ggtgcaaaac
      601 ttcagaaagg gcaacagact tttaattttt ctccaaaata aacccgttac aagggctaca
      661 aggttattca aacctttttc atcgtaaatg caacagtagt accaattcct tccgtactcc
      721 gaatattcac tttctgacca tgggcatcta taatgtgctt aacaatcgct aaaccaaggc
      781 cgcttccacc agtatttgaa gaatggctct tatcgacacg ataaaaacgc tcaaacaccc
      841 ttggcaggtc tttgtccgga ataccaattc cattatcggt aacctctatt aaaagatgct
      901 tgtccatatc gaacaactct accttgacaa agccaccttc ttttgcatag ttgataccgt
      961 tcaagatcag gtttgccaac acctgattta ttagatgcct gtcggcttcg atgcttttca
     1021 ttttttcgta atgcttctta aagctcaggg caattttttt ctcatttgcc ctgtcttcaa
     1081 atgattcaat tacttcagcg attagttttt cgggttcaaa cactttaaaa tcaggcttaa
     1141 tccttccaga ctccagtttt gatattgctt cgaggtcgtt tacaattgaa atcattttat
     1201 tgatattctt ctctatcttt gaaaggtatt tcctgttgat attttcgtcc tcaagcccac
     1261 cttcaagcaa ggtggaagta tagccctgta tgttgaaaat aggcgttttc agttcgtgtg
     1321 aaacatttcc gataaactct ttccggtacg ttgccatttg ccgcagctgc tcaagctcct
     1381 gattatgctc acgatcccat tcattaacat ctgcttcaac ctgatgtatc atatcagcac
     1441 ggttgttgaa ctttgcgttg aaatttcgcc ctgcctcttt ccttttgtga atggctttat
     1501 aaatcacccg aatacggtcg tagataaact gaaaaaaaat aaaccttagg ctaaaaaatg
     1561 agattaaaaa cgacaccgac aacaatatta cacagaaaac catattgaat gaatggtaat
     1621 aatacagatt gaaaaccatt gctatcagca ttattacaac gctgagtaaa accgaaaata
     1681 aaaaagccag cctgttcgat gagtaatatt tcataatatg agcatttgga aattaaacct
     1741 attgttcatc cggtctatat tcaaatttat atcctgtacc tttgaaggtt cgtatgaatt
     1801 catctccgat tttctcccgc aatttcctca catgaacgtc aatggcacgt tctcctacta
     1861 taacctcgtc accccaaata gcggagtata tctcgtccct tgaaaacact tttcccggct
     1921 tcgaaacaag aagtttcagc agtttaaact cttttttagg taaaatcact tcggctccgt
     1981 ttttcattac agagtgcatc tccacatcga tggttagccc attaaatctc aatacggttt
     2041 ccgcctcatc aacatcgctt ctccgattgc ttaatctatt actcctcctc agcatcgact
     2101 gaattttttt aattaaaacc ttgggacgca caggttttgt tatatagtca tcggcacctg
     2161 catcgaatcc ggcaatctgt gagtagtctt cggagcgggc tgtaagaaac acaatcagcg
     2221 tatggtcgaa ctgagttgat gatcttaata tatgacatac ctcaactcca tccatttccg
     2281 gcatcataat atccagcaga attacatcag gattgtattt gccagccaag tcaatggcat
     2341 ccttcccatt tccggcaatt tcaactataa atccctcttt atctagatta tatgacaaga
     2401 attccaaaat atcagggtcg tcatcaacga cgagaatttt ttgaggatta ttcattttgc
     2461 aacattttat tcaattgtaa tttcatatat ttctccggta attgcatttt tggggaacgg
     2521 tatattctcc tgcagatacg gacttccggg ccaccattgt gtgttatgga actcctgagt
     2581 aataattaaa agcatctgat tattcggaat cattgaaatg gattcatcaa caaaacccac
     2641 gacaaacaat ttgtcttttt cgggaccgat gcgccagatg attttatcag gttgccaatc
     2701 tatttggaaa taatagtaat ctcgtccaaa aacttcatcg tccgaaataa agtacttttc
     2761 tatcacagcc cgatatgtat cttcccagcg gaaccagtga aacttcctgc cctggtattg
     2821 aatttcgtta catcctgttc cgaaattgga tggtgatgcg catgccatat cccagttggt
     2881 acagcaaaca gctattgtgt tatcaagcga agcgatatac tcgggcatga gcacattcca
     2941 atcggcaggg tttttctgat tgacaaccgg ctcgttatat aatgggggat attcattatc
     3001 ggggcaatag ggaactgttt tcaaaatttc aaaatcaatt tcggaataag caaccctgtt
     3061 cactcttttc tcttcggtgc caccccagta agttgccatg tatccatttt tttcacacga
     3121 cctccgcagg ttccatccac tttcttctcc aaatccaccc tgataaatca accatattgc
     3181 attggtgata ccattccaca cattgtcttt attcagcaac tctgtcaaat ttgcctttac
     3241 tgttattttt ccatacgtaa aaccatgtct cgttataatg cctacatttt ctttgcgcca
     3301 atctccatag gtagcccctg ggttttgaat aacaatttta ttttccactg aatcagatga
     3361 aaccgtaaga cacggatatt ttgcattggt tggtgtgata ccgataagct cttctttcct
     3421 cgtaaatgca ttattccaat tatattcctc cttactgtac ccatcgttta atacgtcggt
     3481 aattacaggg atattgaaca ttttactaga gcggtcgaca taatgtatga attgctgaac
     3541 agcagcattt tcgtatattg tacgtgaatt attgcaatta catgtgaatg tttcactata
     3601 attcaaagtt tttcgaaaat cgtaatcatg agcatagatt ccggctccca agtcgggttt
     3661 tgcttttaca ttcaacaccg cttcgtattt ttttatgaag ctatctccaa gcttcgctcc
     3721 atctccataa agaaaataat aatacgggtt tacaaaatca ttcttatatg ttttgttgat
     3781 atgttgcaaa taatcaggta tttttttaat atccacctct gttgctatga ctagtaaaat
     3841 actataatca cctactcttg ggtttcgctt ccacctatcg ttcacaccat tgaaataaca
     3901 gtttttttcg tcgcggggat taccaattat tctaaaaaaa tcctgaattt gttttttttc
     3961 tcccggttca atttcaccca caggcttaaa cccgagaaga gtatcctccc atgatccata
     4021 gaaattctca ctagcgtatg gatgcatttg atttgaatta tcagaaactt caggaacttt
     4081 ataagtttcg ttctgataat aaattttata atagtatttt tcctttctgg aagaagtatt
     4141 ctttattgta aattcataat tgaagtaccc agattcagtc tgccgtatgg atggtattac
     4201 aaaaccaagg atggcttccc tgtaatcaag catcaattgt ttgttgtcct gcccgggttg
     4261 gtaattggtc aaccaatgca cattatccac cgaaaatgac agcttttttg cggaaggctc
     4321 tgaagtgcat gaaaaaaaca aggcaatcag caccgctgct gaaacaatat ttaatataaa
     4381 tccgccttta gaacgcataa atctcaactt ttgatttcat catctcctta tcgtgtgttg
     4441 tactttgagt caagttagca gatgaaacat caataaacga aaaacacata atttccggtg
     4501 tctcatcgcc tgtataattt ccacacacaa tactgttgtt tgtatagtat ttcgggttca
     4561 acccatcagg gaatcccttg aagtcaatcg acttttcgat tgtatacatc tttttatcaa
     4621 cgttaatctg tttcagatcg aatctccacg acctgtcgag aactaaaact ttaccagaat
     4681 tgtttttgtg gctaccgata gaaatataat ctgccctagc gtttaacagg taatgatctt
     4741 ccaatatttc tggggtttgg gcaacttgag taatgcgaat tgcgcctttt tgcagggaaa
     4801 ctgaaaatat acggtaaacc attttggttt cttcaccata tattgcaaaa atctcacttc
     4861 ctgctatttc aggcaaaaca tcgtaacaat aaaacttcga aattgagggg aagttatttt
     4921 tttctgttat aaataccggc tctctttttt cggatgggta aatacagcca aatttggttc
     4981 cctgtagcac aagtattgcc ggcttattag ctccggttgt agatatacag gcgccccatg
     5041 tcgttgtaga aaatccatca aagctttcga attgcttttc ccaaagtttt gtccctgaaa
     5101 tctgataagc atcattattc aaactaaagc aaattatttt tctgcctgaa ttatcgatga
     5161 agcaaatttc atctttcttg ttgttatccc aatcaatcgg aatcatcaaa gcattctcat
     5221 taactttttc gctgaatatt acaggttttg agaacaatcc ctctttaact gcataaaata
     5281 ttgacaatgt attgggcgaa ttaccggcta caacctgttc atgctgctga ttcaataaac
     5341 gtatatttga gatacagttg tactttttaa taattccttt ctccggcagg acttttccca
     5401 ttacgtaagt accataactg catggatatg gcggggtgtt gattgcaggc ttaaataccg
     5461 aatcgacagt taagtcgcac agagcattat tacccagttt tatatcgtca gtgtttttca
     5521 taattacgag tatgtcatcc acgtataccg aattattgtt cctgttccat ccataaatac
     5581 acagggtgtt tccgggggag catacttcgg gaggaatttc gaaactatcc tgttctttaa
     5641 tccatttttt taccggacta tttgttttac caagaagata accgtaccaa aaaacatcgt
     5701 tattgttttt atcttttact gcaaaaacca gtacgtagtc catttgggct ttttcactca
     5761 ggtagtaaaa cctgaagttc accctatcga cctgtgttag atcaaaatct gccaatggaa
     5821 tcatggtagg cgcggtatag gtatttttgc cggctgctga ggctgatttc tgactgttgt
     5881 aggcgacacg tgtagtaatt ccatctccgc taatcccttc gtaatcgatt agtttcgatc
     5941 gtttcagtct cgtttgagca tccccatttt gaaaactaat ttcgatatcg tcgatataca
     6001 caatatggcc atctccaaga taattacacc cataaattct gaccttattg ccctgattca
     6061 tcaaggaatc tggaatagta aattgtctgg atgcataaaa ccactctccc gcagtcacat
     6121 tatcgcgatt cacctggagt gcatcccaat acaattgggt ttgcatatct tcatcaaaca
     6181 ccgacagtac aagaagtaca ttcatcttag ttttaacggg cagaaaccat gcgccaattt
     6241 tcacagagcc agtgttggca gatgcaatgt tatcaaggtc gaaaactatg gctgctgagt
     6301 atgcattgcg ctctgccatt accgtgctat gcttccccga cagtgcatgt tctgtactga
     6361 tgccaccttt ggaattttcg agaatgaggt aatctgtttc aaaattaata atgaattttt
     6421 caggaataac ttcggagttt agattgtttt tttttccatc cgtattctta aactcccaat
     6481 gtgctgcaat gtataataaa acagataaca ataatattgc aataaaagta aaaaccaaat
     6541 acagattttt attctttttc agatacggga acgtcatctt tatttggttc aatgttggtt
     6601 tcgttaataa catatagcgg ccggttcctg acgttattgc ctatccggct tatatactca
     6661 ccgataatac ccaggcttat aagttgaatt ccgccgataa agagaacact aataatgaga
     6721 gatgcccagc cctgaacata ttcctttgcc acaatccgct gataaagcgc ccataacata
     6781 ataaaaaacg ataccagact aaaaacaaag cccatatagg ttgcaaattt cagagggaaa
     6841 tcggagaagg aggtaatgcc atcaactgca aatcgccaca tttttttaaa cgaataccca
     6901 gacacaccat cgagcctttt gtcgcggtgg aaggcaacac ttgtttgttt caacccaatc
     6961 catgcgattt gtcctctaag aaacttctgt tgttctggca tttttttcag aaggtttgcc
     7021 accctgcggc taataattct gaaatcaccg gtatccacag gaatgcggct tcgggtaagg
     7081 attgataaaa tacggtagaa aaaccttgct gtcaattttt taaaagcaga gtcacctttc
     7141 cggctttctc ttttcgcata taccacatcg tatccctcat tcattttgtt atagagttct
     7201 cccaatacag ccggggggtc ttgaaaatca gcgtccatga aaacaattct ttttccggag
     7261 caaacttcca ggcccgccgt tattgctatc tgatgtccaa aattacggct taggtcaata
     7321 tatttcacct tgttatcgga aactgacaaa cctttaatta ccggtaacga aaggtctgaa
     7381 ctaccatcgt tgacaaaaat tatttcgtag gattcaatag aattgctttg tagaacggca
     7441 ataatttcgg tgtacagatt gcctatattc ttctcctcgt tcaacaccgg gattattact
     7501 gataaattaa tgtcgtgcaa ctctttcatt attcttcaaa ttaacacaaa tatagtatta
     7561 tttacaatca ttccgatgtc tcattgtcgt tttcagtcac aatatgcggt ctaataagtt
     7621 ctcctattgc acgcagatta aaaatccgaa tcagaaatgc aaataacagc gataaggcaa
     7681 ccgctacaat aaaagcagta aaccaaccgt gaaaaagata gcgacaggca aacgaaattc
     7741 cgatggaaat cgggaaaaac agaattattc tggcaatcaa actataattt gcacgaatat
     7801 tcagcagttt tacagcaata aatatttgcg cagtcatagt aatgagctgt gttaccatac
     7861 tagtaaaagc agcaccagtc gaccaaaaag cagggataag gatgaaatta atactgatgt
     7921 taaacaccat acctgccgcc gccataatgt tcaaatactt caggctgcca tttgcagtga
     7981 gcagagagcc aaaaatataa gtaccacaaa ttccgataaa acctaccata agatagctca
     8041 acacaggggc actacgttcg gcaaatccta catacatcag atgcattagt tcattgttga
     8101 aaacaatgca caccgatgcg agaactatag ccggaacaat aagtaaaagt gccgctattt
     8161 tgattatgcc gccgacattt tcttttttct ggagcattcg ggcgaacata ggcattaata
     8221 ataccgaaaa cagataggct atcatggaaa aagcatccag aatacggaaa gcctgggcat
     8281 atatgccgct ttcttcctgt ccgttgggca acaacctctc tatcataata gaatccaccc
     8341 ggttgtagaa agccataagc agaataagca aagcaaatgg aaaactttgc ttgatgtaaa
     8401 ctgcataaaa cctgtaatcg aaatgtagct taaaatatgt tgttttacga acaacaataa
     8461 tgaatgcgac gatagcagtg attaaataag ctgcagtctg cgagtaaaca aaccattcaa
     8521 ttctgaaagg cactgatgta acatgtcccc acagaagtat tccacaaatc agaatcataa
     8581 taaacctatc aagcaccgaa agaatactat cggttttaaa aaactgaaga ccacttacgt
     8641 tggaacgaag atataatacg aatgaagcga gaaactgatt tacaatgaga acgccaagta
     8701 cactgaattg ccttatgtcg taacccagtg ccaatgcggc aactaacgac acaagtgcat
     8761 aaaatacagc caataacaaa cgtaaggaaa caatgttaga aaaatacttg ctcagcatgt
     8821 ggctgtgcct tgctatattt cggttgttat tattggtaat tcctaagtcg agtaaaatct
     8881 gaagtataag tgaaaaattg aataaagaaa aatacagccc atattcctga gccccaacca
     8941 cattttggac acttcgttcg attccgaaaa tccagaatgg tttgaccaac aagttcagaa
     9001 gcaacagaaa tgcaaggttt attagaaaat ttttcttcaa gagttgggtg ttttgtcttg
     9061 caaatataga aaattgcatt gttactgatt ctatttttaa agtattggct tatgaaaatc
     9121 aagtaaatag tattaagcat tgtaaaacac aaaattttct tatttttgta cgcctttttc
     9181 gggtgtttcc ggaaaattaa aagaaattca attaattaaa acagataaaa ctctgacatg
     9241 aaaacttatc agacgaatca gatcagaaat gttgccctct tgggtaacgg aggttcagga
     9301 aaaacaatgc ttgcagaaag tatgttattt ctaggtggta tcataacccg caaaggtgat
     9361 atcgacagta aaaatacagt ttccgattac agaattctgg agcagaccaa tggaaattca
     9421 attttttcta cggtgatgta cacagaatac aaagacacaa aaatcaatat tatcgataat
     9481 cccggtcttg atgatttcat tggtcaggtt gtttcctcac tcaatccagc tgatctgggt
     9541 ttactggttg taaatgcaca gaatggagtt gaggttggca ccgaaattca caaccggtat
     9601 cttgaaaaag ctcataagcc tgtgattatt gccatgaatc acctcgatca tgataaaaca
     9661 aattttgaga agagtatcga tatgcttcac gagtcgatgg gtggcaatgt tgtggtggca
     9721 caatatcctg taaaccaggg cgtcagcttc gacagtatca tcgatgttat taccatgaaa
     9781 atgtataccc tgaaagatgg caaaaccata atcagcgata ttcctgcttc acaaatgagt
     9841 catgctgaag ggctgagaat gcagttaatt gaaaaagctg ctgaggccga tgaagcactc
     9901 atggagaaat ttttcgaaaa cgacacgctg tctgaagccg aaatgatgca gggtattgct
     9961 gccggattac ctcaccgcgg cattttccct gttttctgtg taaacgcaag aaatgacctc
    10021 ggtgttgaaa gacttatgga ctttctggca ggtgtagctc ctgcccccga tcattgcgaa
    10081 cctataaata acagcgaagg tgaacctgtg aagtgctgta caaaagaaaa gccggcaatt
    10141 ttcgttttta aaacttcaat cgaagagcat attggcgaaa ttaactattt caaggtgatg
    10201 tcgggagaaa taaccgagag catcgacctt tacaactcaa cgaaaggcgt taaggaaaga
    10261 ctctcgcagt tgtttgtttg cgcaggtaaa aacagaacaa aagtgcctaa gcttgttgca
    10321 ggtgatatag gcgccacagt taaactgaaa gccactaaaa catgtcatac gcttgtagcc
    10381 cccggatttg actggacaat tccatcaatt gaatggcctt cgccaaaatt ccgcactgcc
    10441 attaaaacag taaatgaagg tgaagaggag aaattgggag aagcactgaa ccgcatgcgc
    10501 gacgaagacc agacgattgt gttcgagcat tcaaaagaac ttcgtcagtt gattatttca
    10561 gggcagggtg aataccatct gagcattgtt aagtggcacc ttgacaatca gtttaaaatt
    10621 gaaaccaact tcctaaaacc acgaatccct taccgtgaga ccataaccaa aaaagcacaa
    10681 gccgattacc gtcataaaaa gcaatcgggt ggtgcaggac agttcggcga ggtacattta
    10741 atcgtggagc catattccga aggcttgcca gaacccgata tgtacaaaat caataacaag
    10801 gaatacaaaa tttcggtgcg tggtaaagat gaaattgaac ttgaatgggg cggcaaactt
    10861 cagtacttca actgtattgt aggaggttct atcgatgcgc gatttatgcc tgctatcctg
    10921 aaagggatca tggagaaaat agaagttggc ccactgaccg gatcttatgc acgtgatatc
    10981 agagtatgtg tttatgatgg aaaaatgcac cctgttgatt caaacgagat ttcgttccgg
    11041 cttgccggac gtaatgcatt cagcatggcc ttcaaagatg ccggaccaaa aattatggaa
    11101 cctatctacg atgttgaagt gtttgttccc ggcgaccgta tgggtgatgt aatgagcgat
    11161 ttgcaggggc gtcgtgcaat tatacagggt atgagcagtg aaaaaggatt cgaaaaaatc
    11221 atggttagag taccgcttgc cgaaatggga aattactcta ctgctttacg ttcacttacc
    11281 ggaggtcgtg caatgtataa tatgaaattc agcgaatacc agcaagttcc gccagatgtt
    11341 caggaagcac tgctcaaaga atatgcacaa caggatagtg aagaataatt actgttcctt
    11401 ctttataaaa aagaccgctt tttaagcggt ttttttatcc ccctttttta ataatacatt
    11461 tggacattta ttaaacgtaa tgaaaatttt aggctttccc tactggctcc acgtgaatgg
    11521 ttaccgtgag gtgctctaat tgcgtcttaa ggatttcttc gatatcgtcg caatgccggt
    11581 gaacttctgc caaaaccatt ccagcgggaa gttccagatg aaattcaaca aaacgataat
    11641 tgcccgcctt tcgggttttc aggtcgtgat atttcagatt tcgggcagcc agaatttcgt
    11701 taatccggct gacttctgat tcgtcgagcg aaacatcgag caaaggagtg aatgcttttt
    11761 tcagtaactg aaacgattcc cagagtataa gcaaggccac gagaatggca attatggggt
    11821 cgattaatgg tattccggta atccaaagca gtagcaaacc gagcccaact cccagcgagg
    11881 tatatacgtc ggttttcagg tgcagtgcat ctgcctccag ggctaccgaa tcggtttcct
    11941 tcgctacttt atacagcttt cgcgacacga aataatttac cacggccgaa acaagcataa
    12001 ccgttgcccc cagcagtacg aattcaggtt cgcccgggtt ttctatttta tgaatcgcct
    12061 ccacaacaat ccatacggca gcgccgacta taagcaatgc ctctatcact cccgaaacat
    12121 tctcgaattt gccatgcccg tagggatgtc tttcgtcggc aggaatatcg gaaacacgaa
    12181 cggcaaaaaa ggcaatcacc gcagcaacca gatccataag cgagtgtatg gcctccgaga
    12241 taatacttac cgacccgctc aacaaaccca cagttaactt caaagcgatt agcaacgtgt
    12301 ttgacgcgac tgagagcctg gctactgaaa cctttttgtt catcgaataa gaatttcgta
    12361 caaatttaca tggaggattt tttacagaca aaaaaagata ttaccgcgta ttactttgat
    12421 tatcaaatga ttataactaa caattattaa gttattacca gtttatcaat gaaaacgaaa
    12481 gacaaattct ttcaattcag ataattatat tgcaatcctt ttatagacat gtagtgcaat
    12541 taataattac ttttgaaacc aattatcgga atatgaaaac tcttattatt tacgacagtt
    12601 tttttggcaa taccgaaaaa attgcttttg cgattgccaa atcgcttacg aacgaccatt
    12661 ctgttgaaat acacaaagca tcagattttt ctgacttcat caactctgaa tttgatattt
    12721 tgatcctagg gtcacccaca agaggtttta aagctacgcc actggtgcag gcattcattg
    12781 acgactattc attaaagccg ggaaagccaa tggctgtggc tgtttttgat acacgcataa
    12841 aaatcgaaaa tattaagcag ggattttttc gatatatcgt aaaaaaagga ggatttgcag
    12901 ccgataaaat gatgcgggca ttttcgaaaa aacaaataga tacaatagtt gcaccggaag
    12961 ggttttatgt aattgacaag gaaggtccgc tggaagcggg cgaagagcag agagctgaaa
    13021 actgggctca gactatactt gaaaattata ccagaaacac aaacttaaac tgacttttta
    13081 agcttgcctc atctgtgagt ggctgcaaaa aacaaaaaat tgtacgaagc aaaagacctt
    13141 ctaatgtatt cttgccccat gaataaataa gtcaggtctt cacgcaatac tcatcgtatt
    13201 aacctcagga gcaattttct ttaccagtcc ctgcaacaca gttccgggcc ccgattcaat
    13261 aaacatatca gcaccatcgg ctaccatatt cttcacggtt tgagtccaaa gtacggggct
    13321 ggtgagctgt aatatcaggt tttgttttat ttcaacgggg ttggtgtgtg gtttggcatc
    13381 cacgttctga tacaccggac agcggggtgc tgcaaatttg gtgttctcaa tggcagccgc
    13441 cagttcttcg cgggccggct ccatcaccgg agagtgaaaa gcacctccta caggaagttt
    13501 aatcaccttg cgcacaccgg ctgctgttag tttttcgata gcgagatcga tgccacggat
    13561 agttcccgaa attaccacct gacccggaga attaaagttc gccggcacca cgatgtcgtc
    13621 aaccgactga caaatttcgg cgatcaatgc atcgtcggcg ttaagcacag cagccatggt
    13681 tgatgctgcg gcttcgcatg ccttttgcat ggccagtgca cgcttgagaa ccagactcag
    13741 accatcttcg aaactcagtg ctccagcagc tgtcagggca ctgaactcac ccaaagaatg
    13801 accggccacc atgtcgggag tgaaattatt accattggca atggcaagca acaccgaatg
    13861 ggtaaaaata gccggctggg taactttggt ttgcttcagt tcttcgggtg ttccctgaaa
    13921 cataatttcg gtaagattgt aaccaacaat ttcgttggct ttatgaaata atgcggcaat
    13981 cgtactgctg ttgccgaaca aatcttttcc cataccggga aactgtgccc cttgtccggg
    14041 gaaaacgtaa gctttcattt attttaattt tgtgaaatgg atattcagcc tattaatttt
    14101 cattgtgtcc tgttttttaa agaatacaga atgcagaagg ggtggttttt cgggtgaggg
    14161 aaactcgata ttgcgaggac tttttcgaat atcaatacag aggtatctac catcgaattg
    14221 actgtccccg attctgtatt ctgaattctg tattctctca tctgccttca tgaagtttgc
    14281 atttttataa aatttgatca accatcgaaa tataataata cattgtagtg gttggtttag
    14341 ctcaatttcg aagaaataag gtgcaaaaat tctttgcgtg tggcttcttt gcagaaagca
    14401 ccgacaaagt cgctggttgt agttacagaa ttttgcttct gaactccccg catctgcata
    14461 cacaggtgac gggcttcgat aaccaccgca acacccaggg gattcagggt gttctgaata
    14521 cattccttaa tttgtgttgt aagtctctcc tgaacctgca ggcggcgcga ataggcttct
    14581 accacgcgtg caattttact cagaccggtt atgtaatgat tcggaatata ggcaacatgg
    14641 gccttgccaa aaaacggaac caggtggtgc tcgcacaaac tgtaaatttc aatatccttc
    14701 acaatcacca tttgatggta atcttcgtga aattttgcgg aatttaatat tgcctcggga
    14761 tccatgttgt aaccctgcaa cataaattgc atggctttgg caacacgcaa gggtgttttt
    14821 tcgagtcctt cgcgggtagg atcttcaccc agaaggctta taatttcgcg ataatgacca
    14881 ccgatctgtt gtattttttc ggcatcgtat ttttcggtct ttacataccc gcctttcttt
    14941 ttgttgttca gcgattgttc cataactgca attttgttgc aaataaagca atttttcagt
    15001 taatttgttc aaatgaaagt tctgatagta tcggccacac cattcgaagc acagcttccc
    15061 gattcacttt ttcaggtttt taccgcaaaa ggatacaatc ccaacgatgg tcggatccct
    15121 gtgctggtaa gcggtcccgg aattatgtcc tgtgtttttt ccttagcgga gacattgcaa
    15181 cactaccacc ccgacataat tataaacatg ggaattgccg ggtcgctcaa atctgcaatt
    15241 aaaatcggaa cccattgcgt tgtggcatca gattacctga ttgattcagg cattttccgc
    15301 aacaatgttt ttacgcatct gagagattgc gacttctctc tgaacaacga taagcttttt
    15361 cgggctacag ccgatatttt gccacttatg cccgaatgca gcgctgttca tggtcaaaca
    15421 gtgggtctgt tttacgattt gcccttacac caacgtcctg agccgtacca acaaagcgct
    15481 gcagacatcg aaaccatgga aggggctgcc gtgttttatg ttgctgcctc caaaggaatt
    15541 ccagtgtgca gcatacgcac tatttcgaac accgttggca ttgcgcatga acccgggcaa
    15601 ttcttaaatg cggttacatc gaactgtgcg ataattgaaa ccctcatttc acgactcggt
    15661 gcatcttaat tttgccatat cgccctgccc caacgacacc tatatttttc acgcattgaa
    15721 aaatattcag ggctttgcac ccggcataga acctgtgttc acttttgctg atgtggaagc
    15781 actcaacgct atggctctga gcagcgaacc cgatgtttgc aagctgagct ttcatgctta
    15841 cgcccactgc cacaacaatt accgcctgct gggttcaggc agtgcactgg ggcatgcctg
    15901 cggtccgctg ctcatcagca aacgaaaaat ataccccgac gaattgcgcg atgtacacat
    15961 tgccattccg ggcaagctta cgaccgcaaa cctgttgctg ggcattgctt tcccagaagc
    16021 tacacgcaaa acagaatatg tattttcgga tatcgaaacc gtggttctcg atggtgaatg
    16081 cgacgccggc ctcatcattc acgaaaatcg tttcacctac gaaaaaaaag gtcttaaaaa
    16141 aatcattgat ctaggggaat ggtgggaaaa taaattccaa ttacccattc ctctgggcgg
    16201 aattgccatt aaaagaagca ttgcacccca cattgccgaa aatgtagaac aagccattcg
    16261 caacagcatc gtatattcgc acacccacca aagcgaagcc cttgcgtttt gccgcagcct
    16321 gtcgcaggaa atggatgaaa aggtattgca acaacatatc aatttgtatg ttaacgatta
    16381 cagcctcgat ttgggtgaaa aaggcaggca agccataaat tttcttttcg ccagggcaat
    16441 tgaaaccaaa attatcccca acggggttga attgtagaga cgcgataaat cgcgtctcaa
    16501 aaacgcactg acgcggaaaa atcgcttcta aaaaaaacag agacccgata tcgggtctcc
    16561 gcaaaattat attacaataa ttcaccctat ttcaccagtt tcgaaagtac catgtaaccc
    16621 atgagtttgc catttttatc gtagttttcg ctgcgaacca ctcctgcgga ggatgaaatc
    16681 cattctgtta cagaagcctg agtgttcata aaggcaactt tgctggtaac tgtataggtt
    16741 atctttgtag cctgaaatgt accggcagga actgtaatcg attcgctgct aacaactttt
    16801 cggtccgtaa tattgactac catattaaaa acctgaaaac cctgactgct tgctgacatt
    16861 gtaaattttg catcaggaag tgttgttcca ggtacaagcg ttgaagggta ctgcattcca
    16921 acattctcaa ctttcacatc catgtctttg tatgcatcca ttgttgattg gtccatgaag
    16981 ttgctcatat caacgtaaat cacgccattt tcacatttat atgtcatttt cgatgtgaat
    17041 tgatttttct ttgcatcaat tgaattggtt tcgatggtgg cttcgtagcc aactgcagta
    17101 ttgtttactg ccaacacttt tgttgttgca gttgattcga gtttgtcttt agcattgaaa
    17161 ttttgtgttt cccacgacgc accttcatgc aacggataat aatcggggca ggtttgggct
    17221 atggtaaaaa cggcaattac acaggcaatg gtactgagaa atactctttt cataattttt
    17281 tgttttagtt tattgtttgc aaaattaatt cgatttgggc aggttacaaa tattgtgcaa
    17341 aaataattaa cacatcacac atcgttttaa aataactatt taacaaaatt tgtcgtcgcg
    17401 ttcgaaagct gtttcctaaa atatccataa aatcactaac tttgtttatc aaatgaaaaa
    17461 acgagcatga acaaacgcat tgttattatc ggtgcaggaa acgtagcaaa caacctagcc
    17521 tcgctgttac agggcagcgc atacaccata gtgcaggtgt tcagccgcag caacaattcg
    17581 gccgcagcac tcgctcagca atgtgcatgc agccacacct gtaatttcgg caagattacc
    17641 aatgcggccg acatctacat tttctgcctc agcgacgatg ccaacatgca atacattccc
    17701 caatttcgcc acacggcgaa actgttgatt cacacttcgg gcagcatgag ccctgctgtt
    17761 tttgcgggtc ttacagcaaa ctatggcgtt ctctaccctt tgcaatcgct aaaaaaaggc
    17821 attagcatcg ctgcctcgca aattccattt ttaattgaag cctccaacca ggaaagcttt
    17881 aaacaaattg ccgcaatagc agaggctatg ggcgcaactt tcacacatgc cgacaccata
    17941 catcgtacct gggcgcatat ttcggcggtt tttgcctgca atttcagcaa ccacatgttg
    18001 gccattgcct ccgacattgc acgaaaaaac aacgtaccct gggaaatgct tcagcccctt
    18061 atcgtaaata cttttaaccg aagtttgcat gccgacccct ttactgtgca gaccggtccg
    18121 gcttaccgaa acgaaaaatc ggtgatgcaa aaacacagcg aaatgttgca cgacgggttt
    18181 aatacttacg aaaacttgta taaaaccatt agcaatagta tatttgaaat gtataaaaca
    18241 aataaaaagg aagattgatg atacccgatg agagcctgaa tttcaaacag ttattgaaaa
    18301 atataaaagc cattgttctc gatgtcgacg gtgtattgtc gtcgtgccag gtgctgattg
    18361 agcctgatgg agaacttcag cgcaacacca acgtgaaaga cggctttgtt gttcaactgg
    18421 ctacgcgcat gggtatcaga ataggaatta ttacaggagg gaaatcggac gcagtggtaa
    18481 aacgctacaa taaaattggg gtcgacgacg tatattatca ggtgaaagat aaaaacacag
    18541 cgcttgatga atttatgaat aagcacaatt tgaaaccatc agagatttta
//
LOCUS       DAOOQP010000054        18561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3641_length_18561_cov_5.027126, whole genome shotgun sequence.
ACCESSION   DAOOQP010000054 DAOOQP010000000
VERSION     DAOOQP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_18561_cov_5.027126"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLK75_07130"
     CDS             <1..621
                     /locus_tag="PLK75_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOY39057.1"
                     /translation="SPVWIVMSAADEFKDKTTRPNQLWQTDFTYMKIVSWGWYYLATV
                     IDDYSRYIVHWELCKYQKKEDAERVVSDAIIKSGLPPNKRPRLLSDNGPCYIAAGFDE
                     YLASLGVKHIRGRKLHPQTQGKIERYHRTMKNVIKLENYYFPEELEQRIKEFVNYYNN
                     ERYHESINNVTPADAYYRRDKEILRRRKMIKTKTLAERKLSNQIAC"
     gene            complement(693..1124)
                     /locus_tag="PLK75_07135"
     CDS             complement(693..1124)
                     /locus_tag="PLK75_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39058.1"
                     /translation="MKTKLLLLAICLSIANFSQAQLKVVTPGNVGIGTSAPQAKLHVV
                     GNAVFSVNPSLSSTDRAPYIRGSNTYSQASTPSYTWWYNNNTGIFNPSHNIIGFTTDG
                     VERMRINYSGNICIGSTTSQSTHILYVNGLSRFKPNSNDSV"
     gene            1424..2395
                     /locus_tag="PLK75_07140"
     CDS             1424..2395
                     /locus_tag="PLK75_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOY39059.1"
                     /translation="MMNYRNDVEAVDALREKYNLLFKEVSKKIYGQDQVVREVLICIF
                     SRGHTLLVGVPGLAKTLLVNTLAEVLGLKNKRIQFTPDLMPSDIIGTEILDESRQFRF
                     IKGPIFANFILADEINRTPPKTQSALLEAMQERSVTAAGTIYQLDDPFFVMATQNPIE
                     QEGTYPLPEAQLDRFMFNIMVGYPSYKDELVIVKNTTADLDFKLDNVLTADEIVFFQK
                     LVRRVPINDNVMEYAVKLVNMTRPGTGMVHKTAEKYLQWGAGPRASQYLTIGAKANAV
                     LSGKYSPDIEDVKAVAKIILRHRIIKNYKAEAEGISVEDIIDELLNS"
     gene            2408..3922
                     /locus_tag="PLK75_07145"
     CDS             2408..3922
                     /locus_tag="PLK75_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOY39060.1"
                     /translation="MRHIVFFLLVSICTWQSAFSQNKRKIEIVDANSLEYNQNIGRGA
                     KRLIGNVVFKHEGAMMYCDSAYFYSESNTVDAYDNVKIEQGDSLMLTGTTLKYNGNTK
                     MAIVRKKVVLSHFNSYLETDSLNFDRSLNMGYYFNGGNIYDGDNHLFSKRGYYYADDK
                     DYFAVDDVVLTNPEYVIYCDTLRYNTETQIAYFYGPTRIVSDSTFIYCENGNYDTKSD
                     LASFSENAYLVSGQQTLKGDSLFYNRNIRMGKAFRNVSVTDTVENVIAEGNLGYYYEN
                     PKKAMLTDSVLVTYISDGDTVFMHADTVYVDIDSLDRKLVRAFRKVQVFRNDMQARCD
                     SLVYCTADSVAEMFGKPVLWSNESQLTGEYMVMHFENNKAHHVEITGYALIVQEEDSA
                     RYNQIQGRKLVAYIKDNDVWRIDIFNDSQTIYFIRDEKTNDITGLNKIISSDMIIYRT
                     DKQIEKIQFFEKPDGIVIPIKDLTESDAFLKDFVWLDVFRPKNKLDIFNWISIK"
     gene            3957..4469
                     /locus_tag="PLK75_07150"
     CDS             3957..4469
                     /locus_tag="PLK75_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39061.1"
                     /translation="MSEKLLIGVLGHYHAGKSTTWNSLFQRVVRTGKHERTLVIGSMD
                     IPVFLINGAPLERKTELKKILPKDDPTIVLCSFLYHKNVIQNFEYFIKKGYQIYIQWL
                     NPGFSDEYDASLFYQYGIIDYLLNSGATVSVQNGKINPEKRVEMVQNYLLGWYYFYIE
                     KNCEKILNFG"
     gene            4713..4976
                     /locus_tag="PLK75_07155"
     CDS             4713..4976
                     /locus_tag="PLK75_07155"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOY39062.1"
                     /translation="MTLLIESQNIGKVLELKLHSIGIDTIETLKDVGTEETFRRLKEI
                     DKNCSRSILFAIEGAIQDVRWHKLEEYRKNELRAFYNEIDNVY"
     gene            5140..8373
                     /locus_tag="PLK75_07160"
     CDS             5140..8373
                     /locus_tag="PLK75_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY39063.1"
                     /translation="MYKIEKHPILDVPVNEKLTFHYNGKPITGDSGFTIAAALHQAGF
                     PVHSHSLDGRNRSLECGIGKCGACEMLVDGQIKRICITKIDGVQDVREIPMNHMPPKE
                     YAISDKPVNTYKTTVAIIGAGPAGLAARELLNENNVANIVIDNNDKIGGQFLMQTHQF
                     FFFEKEKKFGGMRGFDIAKTLAGDDYTGIMLNSTVWDILEGKRIAVKNIKTSEVFYVI
                     ADFLIVATGAIPFMPSFGNDDVPGVYTAAVVQKMMNNEYTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGARVKAIIEAQQFEGGFPVQANRVRRLGIPIMTGYILVEAIPNSDYSGIIG
                     AVIAKCENFKAIPGTEQAIYGIDTINICTGLVPDDQLAVKGELVFGRNLFTVGDALRI
                     GEGTSAVLRGKQSAYEILERMGARFRFDDFLNISKEYIDSMQHPVRVIEEAYKPTRER
                     AESRPYVLIDCLYGFACNPCAFACPHGAITKSSTSTVPRIDFDKCIGCMDCVYQCPGL
                     AIFGYNEKKDWLFLPIEYEVTEGAEVFLVDNNGKKLGQGIVEKILRKTNKTNVARVKS
                     LDIHGIDLANARGFIVKENYPGAPDIKDLNKTYATECYICHCDDVKLEEIVNVIGNRK
                     YISIDEVKHTTRLGMGACRGKRCIKRLKQIIAPYGIEIVGEATPRGPLSNQLSLGEMY
                     PKSKNEKIVPNINGKQIKRVKVQALVAGGGIAGSAAFRYMSEVGMKPVLINKDRGASW
                     RNIAGGRPAFSLPALADIALKNKAIFEDLEKKSGINYHPIRYVSFAHDEANYRALEAS
                     IAWSNAYMVEPKDFRKEISPHFNQNLKTYLSALVTHDCWQATPGKVLDLIRRIGIEKG
                     GSILEDCALIDLHREGKNVVALVLNHNNEYVEYETEHFVNALGPEAGVFANKIGIETG
                     LYPVKHQAFITKRLPMMGKDGAQLDMIIDRRKYKGFSAVYGQQLAETGQIIGCASPAV
                     DPLETRQELKVNNKEFIEIISEVFVDWLPALANVSVQAVWAGYYVEPRYIVDPDHGLI
                     VGLRGHGFMLGQYLAKMYVDKLMGKPVPDYFEELKLGGPGLSEAAFK"
     gene            8556..8879
                     /locus_tag="PLK75_07165"
     CDS             8556..8879
                     /locus_tag="PLK75_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOY39064.1"
                     /translation="MKIIEQHIESLRKLCEAYHVSVLYVFGSVSEGNFTETSDIDFLV
                     RFESVDPLEYFDNYLDFKTDLQKLFSREVDLLEIQTLKNPILIRSIEKNKILLYGREN
                     SKMAV"
     gene            8848..9195
                     /locus_tag="PLK75_07170"
     CDS             8848..9195
                     /locus_tag="PLK75_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOY39065.1"
                     /translation="MEERIQKWLFDIKYAIEEIDNFFLENPKEFSVYSTNLILKRAIE
                     RNLEIIGEAVNRILKADPQFPIKNANKIVGLRNQIIHAYDGISDENIWAVVQKHLPLL
                     QEEVNRLIDQLQS"
     gene            9309..9533
                     /locus_tag="PLK75_07175"
     CDS             9309..9533
                     /locus_tag="PLK75_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39066.1"
                     /translation="MGKLFLITLVLMAIAFLALGIAIFFGKKKKFPEGSISKNKDMQQ
                     RGITCVKHEEYHSCGLNGGCCGNHEVAKND"
     gene            complement(9552..10001)
                     /locus_tag="PLK75_07180"
     CDS             complement(9552..10001)
                     /locus_tag="PLK75_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39067.1"
                     /translation="MKTKPLFTLAFILIVSFSSLFAQNADYDLVVSVFTKGTYKIWEY
                     KLNYKSVTVTRFSTNGDAPEVVFEKRISMNEGPVFDELLGDFPYETVKDKYINPEVEG
                     ENHYEITVRHQHKSKQVYVYFEYPSELKILIDALNTLLPENYQLVFE"
     gene            10245..11168
                     /locus_tag="PLK75_07185"
     CDS             10245..11168
                     /locus_tag="PLK75_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39068.1"
                     /translation="MNRFLIPVYTLLVAVSFLLTSCKPDPADPPTIELIFETGMAQEG
                     DTFAVGRPIRFRIVANGADANITNFLVQKVFDGTHKTVLDSGLNSAGFTVTETYYQGV
                     EEEVEWTFSVMDRNRKSASVKIKLIKDPNSQFGGILEYDSITVGLQSNPDVGHFFLPS
                     LNEVYFEDSATMFQDLVDVLCYFNYSEDNGVNLPSPTFSSPGEDPNASGYLYSEYYPF
                     ISSWATRNYTKYDIRADNGVTLQEFFSSHNDSLLIVSYDDVWGKKKYKWAYSGTLIPF
                     MTAGGKKGIIRVIEADTVATGTITFSMKIQL"
     gene            11192..12601
                     /locus_tag="PLK75_07190"
     CDS             11192..12601
                     /locus_tag="PLK75_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39069.1"
                     /translation="MKKNLLFVFAFLMLFSPIIVAQQQVMIGFTFSDNTDADFTADNG
                     AGLPGNDSYNVRAENAAGDTRDLIYSAGATTYAATADNWDAGSDDKFWSIKFKADGYQ
                     NFKVSSKQYSDLTGPKEFKLQYKMGNGGTWTDITGGTVTVANDWASGVVNEISLPAEL
                     NDPGTTSIYIRWIMTSNTSVSNATVEATGASKIDDIMVYGSPLPTPVSIIGFNFTDNT
                     DEEFNADLGLAGNLTYDIRAENTAGDTRTLTYTEGATDYAATASGWDAGSGDKFWSIK
                     FKADGYHGMKVSSKQYSEATGPKDFKLQARFSGGDWVDIDNGTLTVASDWSTGVAEEL
                     DLPASFDNPGTTSIYIRWIMTSNDNVENGTVDADGISKIDDILVTGFNSTGFESVIFE
                     NLVSVFPNPCNSHVNIAANEEIAYCSLYDMQGKLISTTRVDADETTISTSNLAKGMYI
                     VRVFFGNEKTPVTERIIVE"
     gene            12670..15000
                     /locus_tag="PLK75_07195"
     CDS             12670..15000
                     /locus_tag="PLK75_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39070.1"
                     /translation="MRKILTILLLLLNVYSFSQTISGYVTDSLRNIMPNANISLNRHE
                     KGTITDSNGYFKLSLEKPGKYILKASFIGYEPAIKQIELNDNQNITIDFVLIPSTIFT
                     PEVEIQAKKTDGFDHFDVPIRVKTLNTYDLVQIPAISSSKLFNNISGIDVRNEFGIFS
                     SQTVVSLRGLGGGQSGTLVVIDGTPLNKSESGSVNWNMIDIDGIEKIEIMKGPGSVLF
                     GSNAMGGVILITTSKPKSLFSADMSLGYGRYNTMETKLNTSGRSLNNLIYWKANIMFR
                     RSDGYVYTPDYEIIENDTIIVPVYLREYSAGAMLGFQINEQNRLELSFDYYDDRRGSG
                     IKIFEDLGANDEHDTYSTYLRYKGNYRKFSLYSNLYLLNENYGKLNEYYSDGEYKLYE
                     VDSKRLDYGAKIWTEIPVSSKCDITAGVDFKTGAVNASDIYFTSTDVIRNRGEMNTLA
                     AYLQLKYYITPHFTAIGGVRYDIALFHDAAFSIEQPSYSIEYLAGYQFSDVETEHWSA
                     TNPKFTLQYTINEFDKIYVTVAKGFGAPILEDLCRTGRKKIGFKIANPALQPEHITNF
                     ETGFDFGLGKKLNFAASFYYTLGDNFIYFVTTGDSVNMGYTIAPIYQAKNISKVEIFG
                     AEADINVALTNNLKAFGNYTYNHSQIKEYIANLEQGDKDLSGKFLTDVPMHKFSLGLS
                     FQNKYVNISSSVKYTGERWLNDENKYDNVYLMSDKYPDYFLVDMKLWKQIRSLTLSVN
                     IDNLLNTIYTDSKGFKSPGIFWMIQVKYNFVTPKTK"
     gene            15003..16088
                     /locus_tag="PLK75_07200"
     CDS             15003..16088
                     /locus_tag="PLK75_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39071.1"
                     /translation="MKKIKVVYYLSVILIVMASCAGNSNRGKQEVGNSSQQTVQHDTA
                     DYTNNFYDNAEKYSLKTYDLEVVGEVSNPGKVDFSKLPLRSVIVKETLLGDNGDKFVG
                     AYCYDGYSLFDILNAYHVAKKNEEEYKPIIDMYVEIENADGEKVVFSWGEIYYPNHLH
                     EIIIATNVMRIVPSKTNELWELPTDNKLVVVSDLITERNISKPVKITVKSYDKQLVTI
                     KGKTPLFAPKFDFFNSDKLIATYTQNPKDIAELTLHTIFYGRGRGIHSTQPFTGVPLK
                     QLIAGHIPLTQTSIRQGLFLAAADDGFRAVYTYSELCNRNDQADVLLICHPEVTTDGI
                     FRLFPSCDFFSDRAIKALNALYYSEAK"
     gene            16185..16967
                     /locus_tag="PLK75_07205"
     CDS             16185..16967
                     /locus_tag="PLK75_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY39072.1"
                     /translation="MKNFSAFTLTLWLLGGMVLLFIAAPLLGLFLSTGAPKLFDTIQD
                     REVTRSISLTLWASMAATIIFAIGAIPLAWLLARRKFRFKGLVNALIDLPIVIPHSAA
                     GIAILGLVSRDTLLGRIGESMGLSFVSNPAGIIIAMAFVSLPFLINAARDGFSAVPEK
                     LEKAAYNLGASPMRVFFTISLPLAWRSIVSGLIMMFARGMSEFGAVVIVAYHPMTTPV
                     LIYERFGSFGLSYAQPVAVIFISICLLFFALLRYFSNRTALD"
     gene            16960..18009
                     /locus_tag="PLK75_07210"
     CDS             16960..18009
                     /locus_tag="PLK75_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY39073.1"
                     /translation="MIELKNISKKFRNFSLKSINLQVEQGSYFVLLGNSGSGKSVLLE
                     TIAGFYSPDSGQIFINKKDVTNLSIAKRKTALVFHDSALFPHLTVRGNLMYSLKTGKI
                     QRSKWDSLVNDMASLTEVSQLLDRYPAKLSGGEKQRVALGRALLQKPHVLMLDEPLAS
                     MDVTLRSNMRSLLRKINRSGQTIIHVTHDFEEAAMLATEIAVIENGSIMQCGKPAHVF
                     QSPKSEFIARFSGYKNFFEGNIVNNNGHSVFRTKNIEIVLSDSEAVGYGFAAIRSEDV
                     SIGIEKPISSARNNYRGQVTSIEQYHSNYEIIVDAGQEFSAYITAEAMKELALDVGSW
                     VWVSFKAFSVKFIEK"
     gene            18006..>18561
                     /locus_tag="PLK75_07215"
     CDS             18006..>18561
                     /locus_tag="PLK75_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39074.1"
                     /translation="MIDYKEAAIIIQRAVESVSTETIPYSIALHRIIAQDIVSDVNMP
                     PFDKSAVDGYACRRSDLGNKLMLKDVIRAGQEHHIVIDKNECAKIMTGAMIPAGADCV
                     FMVEQSEISEDKMIRFIGIKTSDNIARFGEDVKTGSVVLRKGTFIEPKHISVLAATGT
                     TMIEVFRKIKVAIITTGDETVEPDI"
BASE COUNT         5857 a         3332 c         3921 g         5451 t
ORIGIN      
        1 agccctgttt ggatagtgat gagtgcagcc gatgagttta aagacaaaac aacaaggccc
       61 aaccagttat ggcagaccga ttttacatac atgaaaatag ttagttgggg ctggtattat
      121 cttgccacgg taatagacga ttattcacgt tatatagttc attgggagct ttgcaagtac
      181 cagaaaaagg aagatgccga aagagttgtt agcgatgcaa taatcaagtc aggcttgcca
      241 ccaaacaaaa ggccaaggct attatcggac aacggcccat gctatattgc tgcagggttt
      301 gatgaatacc tcgcaagcct gggagtgaaa cacatcagag gtcgcaagct tcacccacaa
      361 acacagggga aaatagagcg ctatcacaga acaatgaaaa atgtaatcaa gcttgaaaac
      421 tattattttc cggaggaact agaacaaaga atcaaagagt tcgtaaacta ttataacaac
      481 gaaagatacc atgaatcgat aaacaacgta actccggctg atgcttatta tagaagagac
      541 aaagaaatac tacggagaag gaagatgata aaaacaaaaa cattagcaga aaggaaatta
      601 agtaaccaaa ttgcatgtta aaattaaacc aattggaaat aacaaaaata caccttaact
      661 ttgataagaa ttttgtccaa catgttcaga cttcatacac tgtcgttaga atttggttta
      721 aatcgtgaga gcccatttac atataaaata tgtgtagatt gagatgttgt tgacccaata
      781 caaatatttc cactgtaatt tattctcatt ctttccacac cgtctgtggt aaatccaata
      841 atattatgcg atgggttgaa aatgcctgtg ttattgttgt accaccatgt atagcttggc
      901 gttgatgctt gtgaatatgt atttgaaccc cgaatgtaag gtgccctgtc ggttgagctt
      961 agcgatggat ttactgaaaa tacagcattt ccaacaacat gtaatttagc ttgtggggca
     1021 ctggtgccaa ttcctacatt gcccggagtt acaactttta actgggcttg gctaaagttt
     1081 gcaatagaaa ggcaaatggc taataagaga agttttgttt tcataaatat tgatttattc
     1141 ctacaaatgt aaaaataaaa cattaaagaa tacaaaataa ttatacgtta atattatgca
     1201 cgttaatatt acgtgttaaa tattgtcttt ccctgttcaa tctgtaattt tcttgttttg
     1261 cgttgcccat ccatttcaaa ttctgcaacg tacaaaccct cagccaatta tggtaggaca
     1321 atgtagcagt ttttgggaat atattctaca tgcagcataa atataagata aaaatttttt
     1381 cgtttatatt ttcgttgtaa atttgctcac ttaaaataaa aaaatgatga attaccgcaa
     1441 cgatgttgaa gctgttgatg ctctcaggga aaagtataat ctgttattta aagaggtaag
     1501 taaaaaaata tacggacaag atcaggttgt aagagaagtg ctgatatgta ttttcagtcg
     1561 cggccacaca ttgcttgttg gtgttcctgg tctggcaaaa acacttttgg ttaacacctt
     1621 agctgaagta ctgggtttaa agaacaaacg tattcagttt acacctgacc tgatgccttc
     1681 cgatatcatt gggaccgaaa ttctagatga atcaaggcag ttcaggttta ttaaaggacc
     1741 catttttgcg aatttcattc ttgctgacga aatcaacaga acaccaccca agactcagtc
     1801 cgcccttctc gaagctatgc aggaacgctc ggtaactgca gcgggaacta tttatcaact
     1861 cgacgaccct ttctttgtaa tggcaacaca aaacccgatt gagcaggagg ggacttaccc
     1921 attgcctgag gcccaactcg accgttttat gttcaacatt atggttggat atccaagcta
     1981 taaggatgag cttgtaattg ttaaaaatac taccgcagac ctcgatttta aactcgacaa
     2041 tgtgcttact gcagacgaaa ttgtattttt ccagaagctg gtgcgacgcg tacctataaa
     2101 cgataatgtg atggaatacg cagtaaagtt ggtaaatatg acgcgtcctg gaaccggaat
     2161 ggttcacaaa actgcagaaa aatatctgca atggggtgcc ggtccacgtg cttctcaata
     2221 tctcacaatt ggtgctaaag ccaatgcagt gctgagcgga aagtactctc ccgatattga
     2281 agatgtgaaa gcagtcgcca aaatcatact gcgccacaga attattaaaa actacaaagc
     2341 agaggcagaa gggatatcgg tagaggatat cattgatgaa ttacttaact cgtaagaaat
     2401 gcctggcatg aggcatatcg ttttttttct gttggtttcg atatgtacct ggcaaagcgc
     2461 attttcacaa aacaaacgta agattgaaat tgttgatgcc aatagtctgg aatacaatca
     2521 aaacattggc aggggtgcaa aacgtcttat tgggaatgta gtgttcaagc acgaaggtgc
     2581 catgatgtac tgcgacagtg cctattttta ttccgaatcg aatactgtcg acgcttacga
     2641 taatgttaaa atcgaacagg gcgacagcct catgcttacg ggtacaacgc ttaagtacaa
     2701 tggaaataca aaaatggcta ttgtacgtaa aaaagttgta ttaagccatt tcaattcgta
     2761 tctcgaaacc gattcactga acttcgacag aagcctgaac atgggatatt attttaatgg
     2821 tggaaacatc tacgatggcg ataatcatct tttcagtaag agagggtact attatgccga
     2881 tgataaagac tattttgccg tcgatgacgt agtgcttaca aaccctgagt atgtaatcta
     2941 ctgcgatacg ttacgctata acaccgaaac tcaaattgcc tatttttatg gtcccactcg
     3001 tattgtcagc gattctacat tcatctactg cgaaaacggg aactacgata caaaaagcga
     3061 cctggcatca ttttccgaaa atgcgtatct ggtttcgggg cagcagacgc ttaaaggtga
     3121 cagccttttt tataaccgca acatccgtat gggtaaagcg ttccgtaacg taagtgttac
     3181 agataccgtt gaaaatgtga ttgccgaagg taatctggga tactattacg aaaaccctaa
     3241 aaaagccatg ctcaccgata gtgtgttggt aacctatatc agcgatggcg atacagtttt
     3301 tatgcatgcc gacacagtgt atgttgatat cgacagcctc gacagaaagt tggtaagggc
     3361 attccgcaaa gttcaggttt tcaggaacga tatgcaggca cgatgcgatt cactggtgta
     3421 ctgcactgct gattcggtag ccgaaatgtt cgggaaacct gtgttatggt ccaacgaaag
     3481 tcagcttaca ggtgaataca tggtaatgca ttttgagaac aataaagcac atcacgttga
     3541 aataacaggg tatgcactta ttgttcagga ggaagacagt gcgcgctata atcagattca
     3601 gggacgaaag ttggtggctt atataaagga taatgatgtg tggcgaatcg acatttttaa
     3661 cgatagtcag actatatact ttatccgcga cgaaaaaacc aacgacatta cagggctcaa
     3721 taaaatcatc agctccgata tgatcattta tcgtacggat aaacaaatcg aaaaaattca
     3781 attcttcgaa aaacccgatg gtattgttat tccaattaaa gatttaactg agtctgatgc
     3841 ctttctcaag gattttgtat ggctcgatgt attccgtcct aaaaataaat tggatatttt
     3901 taattggatt tcaataaaat agtttatttt tatagagcca accaatcttt caatttatga
     3961 gcgaaaaact gcttataggt gtattagggc actatcatgc aggaaaatcc actacatgga
     4021 acagtctttt tcaacgggtt gttcgaaccg gaaaacacga aagaacgctt gtaatcggga
     4081 gtatggacat ccctgtattt cttattaacg gtgctccgct ggaacgcaaa accgagttaa
     4141 agaaaatcct tcccaaagat gatccgacta ttgtgctgtg ctcttttttg taccataaaa
     4201 atgtaattca gaattttgag tattttatta aaaaagggta tcagatttat attcagtggc
     4261 ttaaccctgg tttcagtgat gaatacgatg caagtttgtt ttaccagtat ggtattattg
     4321 actatttact caattccgga gcaacagtaa gtgtgcaaaa cggtaaaatc aaccctgaaa
     4381 agagagttga aatggttcaa aattacttat tgggttggta ctatttttat atagaaaaaa
     4441 attgtgaaaa aatattgaat ttcggataat agaaactatt ttttagtgtt ggatttgacc
     4501 cagtaaccga ataaaacaat tctaaaaaac ttgctttaat gctgaagtag gtcatttatt
     4561 tattaattgt tctatactat ctgttattgt tttgcattta aaatgtggaa aagaaatatt
     4621 tgatataaaa acagaataaa gcgttttttt ttactttgaa attcaaactc aaaaacaata
     4681 tatttgcatt caattagaaa atattaataa ttatgacatt acttattgaa agccaaaaca
     4741 taggcaaagt gctggagctt aaacttcata gtattggaat cgacactatc gaaacattaa
     4801 aggacgtggg cactgaggaa actttccgtc gtttgaaaga gatagataaa aactgtagta
     4861 gaagtatttt gtttgcaatt gaaggagcaa ttcaggatgt gcgctggcat aaactcgaag
     4921 agtatcgaaa aaatgaactt agggcttttt ataacgaaat agataacgtt tattaataaa
     4981 ttcagatttt ctgaataagt ttggcatttt cttaatgctt cagggctata tactctgaag
     5041 cattttttat tatctgtaat gctgacaaaa ctcatattct aaggggaaaa ctattgtcaa
     5101 ttttacctgc tttttaatgg ttgatttaat agattaatta tgtacaaaat agaaaaacac
     5161 ccaattcttg atgttcctgt taatgaaaag cttacatttc attacaacgg taaacctatt
     5221 acgggcgact ctggtttcac cattgctgct gccttgcatc aggcaggttt tcctgtgcat
     5281 agccattccc ttgatggacg taaccgctct ctggaatgtg gtatcggaaa atgcggagca
     5341 tgtgaaatgc tcgttgacgg gcagattaaa agaatttgta ttacaaaaat cgatggggta
     5401 caagatgttc gtgaaatacc aatgaaccac atgcctccta aagaatatgc aatatccgac
     5461 aagcctgtaa atacctataa aacaaccgtt gcaattatag gggcagggcc tgcaggactc
     5521 gctgctcgtg aattgttaaa cgaaaataat gttgcaaaca ttgttatcga taataacgat
     5581 aaaattggcg ggcagttttt aatgcaaacg caccagtttt tcttctttga gaaagaaaaa
     5641 aagtttggcg gtatgcgtgg ttttgatatt gcaaagacct tggccggaga cgattacaca
     5701 ggcattatgc ttaattcaac ggtatgggat attctcgaag gaaagcgcat tgcagtaaag
     5761 aatattaaga cttcggaagt attttacgtt attgccgatt ttctgattgt tgccaccgga
     5821 gccattcctt ttatgccatc ctttggtaat gatgatgtgc ccggtgttta tacggctgcg
     5881 gtagtgcaaa aaatgatgaa taacgaatat accttgttgg gtaaaaacgt gcttaccgtt
     5941 ggagctggca atataggata tctcacttcg tatcaattaa tgcaggcagg agccagagtg
     6001 aaagccataa tcgaggcgca gcagttcgaa ggcggttttc ctgtacaggc taacagggta
     6061 cgacgcctgg gaattcccat tatgaccgga tatattctgg ttgaagctat tcccaatagc
     6121 gattattcgg ggataattgg agcagttatt gcaaaatgcg agaattttaa agccatacct
     6181 ggaactgaac aggcgatcta tggtatcgat acgataaata tatgtactgg acttgtgcct
     6241 gacgaccagt tggcagtgaa aggcgagctg gtgtttggca gaaatctgtt tacagttggg
     6301 gatgctctac gcataggtga aggcacaagt gcagttttac gcggcaaaca atcggcatat
     6361 gagattctcg aacgcatggg agcaaggttc cgcttcgatg acttcctcaa tatttcgaaa
     6421 gaatatatcg attcgatgca gcatcctgta agggtaattg aagaagctta taaacctacc
     6481 cgcgaaagag ccgaatcccg tccatacgtg ttgattgatt gtttgtatgg ttttgcctgc
     6541 aacccttgtg cttttgcttg tcctcacggt gcaattacta aatcttccac tagtacggta
     6601 ccgcggatcg attttgataa atgcatcggg tgtatggatt gcgtttatca atgcccggga
     6661 ctagcgattt ttggttacaa cgagaaaaaa gactggttgt ttttacctat tgaatatgaa
     6721 gttactgaag gagctgaggt ttttctggtt gacaacaacg gtaaaaaact tggacagggt
     6781 atagtagaga aaatactgcg taaaaccaat aaaacaaatg tggctcgtgt aaaatcgctt
     6841 gacattcatg gcatagacct cgccaatgcc cgtggtttta ttgtaaagga aaactacccc
     6901 ggtgctcccg atattaaaga cctgaataaa acctatgcta ccgaatgtta tatctgtcat
     6961 tgcgacgatg taaaactcga agaaattgta aatgtaatcg gcaatcgtaa atatatatcg
     7021 attgacgagg taaaacacac tacccgactg ggaatgggag cctgtcgggg aaaacgatgc
     7081 atcaaaaggc tgaagcagat aattgcacca tacggaattg aaattgtagg agaggcaaca
     7141 ccacgtggtc ccttatcgaa ccaattgagc ttgggagaaa tgtacccgaa aagcaaaaac
     7201 gaaaaaattg ttcccaatat taatggaaaa cagattaaac gcgttaaagt gcaggctctt
     7261 gtagccggtg gtggtattgc aggaagtgct gcatttcgtt atatgtccga ggtaggaatg
     7321 aaacctgtac tcattaataa agaccgtggt gcttcgtgga ggaatattgc cggtggacgc
     7381 cctgcgttca gcttacctgc tttagccgat atagctctta agaataaagc catttttgaa
     7441 gacctcgaaa aaaagtctgg tattaattat catccgatac gttatgttag ctttgcccat
     7501 gacgaagcca actatcgtgc ccttgaagct tcaatagcat ggagtaatgc ttatatggtg
     7561 gagccaaaag atttcagaaa agagatttct ccccacttta atcaaaacct gaaaacctat
     7621 ctttcagcac tggttacaca cgattgctgg caagctactc cgggtaaagt acttgacctg
     7681 atacgcagaa taggtattga aaagggaggt tcaattcttg aagactgcgc tctaatcgat
     7741 ttgcaccgtg aaggtaaaaa cgtggttgca ctcgtactta accacaacaa tgaatatgtg
     7801 gaatatgaga cggaacattt tgtaaatgcc ttgggaccgg aagccggggt atttgccaat
     7861 aaaattggca ttgaaacagg gctttaccca gtaaaacatc aggcattcat aacaaaacga
     7921 ctaccgatga tgggaaaaga cggtgctcaa cttgacatga taatcgaccg cagaaagtat
     7981 aaaggttttt cagcagtata tggacaacaa ttggctgaaa cagggcagat cattggttgt
     8041 gcatcgcctg ccgtcgatcc tctcgaaacc cgccaggaac ttaaagttaa taataaagaa
     8101 tttattgaaa taatatcaga agtgtttgtt gactggcttc ctgctttagc aaatgtctct
     8161 gttcaggctg tatgggctgg ctactacgtt gaaccacgat atatcgtaga ccctgatcat
     8221 ggtctgattg tgggtcttcg cgggcatgga tttatgcttg ggcaatattt agctaaaatg
     8281 tatgtcgaca aacttatggg aaaaccggtt cctgattact tcgaagaact gaagttgggt
     8341 ggtcccggac tttcggaagc agcgtttaaa tagcggaaaa gcttgctttt atttgttggt
     8401 tgaattataa aatcttattt gaaattgatt ttcaaatatg taaatgtgca aattacttta
     8461 ttgaaaacta cgaaatattc ttaatacatt attattgaat ttacgagtgt taaaatgctt
     8521 gcatgggttt cgttttattt tgtatctttg ttaagatgaa aatcatagag cagcatatcg
     8581 aatcattaag aaagctatgt gaagcatatc atgtttcagt tttatatgtc tttggttcag
     8641 tttcggaagg aaattttacc gaaacaagtg acattgattt tttagttcgc ttcgaaagtg
     8701 ttgacccatt ggaatatttt gataattacc ttgattttaa aactgactta caaaagcttt
     8761 tttcgaggga ggttgatcta ttagagattc aaacactaaa aaatccaata ttaataaggt
     8821 ctatcgaaaa aaataaaatt ttgctctatg gaagagagaa ttcaaaaatg gctgtttgac
     8881 attaaatacg ctattgaaga aatagacaac ttctttttag aaaatcccaa agaatttagt
     8941 gtttactcga ctaacttgat tttaaagagg gccattgaaa gaaatttaga aatcataggc
     9001 gaagcggtaa acagaatatt gaaagcagat ccccaatttc caatcaaaaa tgcaaataaa
     9061 atcgtagggt tgcgaaatca aataattcac gcatacgatg gcatatccga tgaaaacatc
     9121 tgggctgtag tgcagaagca tttgccgttg ctgcaagaag aggttaatcg gttaattgac
     9181 caattacaat cataaacttt gcatgatttt aaaattacct aatcatttag tttctatttc
     9241 caccttcagc acaaaattcc acaacaaatt gtacttttat ctgtttaata atatgaatga
     9301 aatgtattat gggaaaatta tttttgataa ctcttgtgct gatggctatt gcctttttgg
     9361 ccctgggtat tgctattttt ttcggtaaaa agaaaaaatt tcccgaaggc tccatcagta
     9421 agaacaaaga tatgcagcaa aggggcatta cctgtgtgaa acacgaagag tatcattcct
     9481 gtggattaaa cggggggtgt tgtgggaacc atgaagttgc taaaaatgat tagaaacaat
     9541 ctttataaaa attattcaaa aaccagttga tagttttcgg gtaaaagagt attcagtgca
     9601 tcaatcagga ttttaagttc ggacgggtac tcgaaataca catacacttg tttgcttttg
     9661 tgctgatggc gtacggtgat ttcataatga ttttcacctt caacctcggg gttgatatat
     9721 ttatctttca ccgtttcata cggaaaatcc ccgagtaatt catcaaatac cggtccttcg
     9781 ttcatcgaaa tgcgcttttc gaaaacaact tcaggggcat cgccgtttgt cgaaaacctt
     9841 gttactgtta ctgatttata attcagtttg tattcccata tcttatatgt accttttgta
     9901 aaaaccgata caacaaggtc gtaatcggca ttttgggcaa aaagtgagct aaagctaaca
     9961 atcagaataa atgcaagagt gaataggggt ttcgttttca tggtaacctg atttattatg
    10021 gtttatagtg tgtatatgag attcaaatat caaatttagt gttttagcat taatattctg
    10081 cgcttttatt taatctttta ataattagca gacaccattt atattgtctt ataataacgc
    10141 aaattttcct gaattgtcca atttcatttt tctattaatt aaatattttt acatttgcag
    10201 ggaattgagc atgtgtgcaa ttcgctttag ttacatgtaa tattatgaat cgatttttaa
    10261 taccagttta tacgctgctc gttgccgtaa gctttctgtt aacatcctgt aagcccgatc
    10321 ctgccgatcc tccaaccatt gagttgattt ttgaaacagg tatggctcag gagggggata
    10381 cctttgccgt gggtcgcccg attcgtttcc gtattgttgc aaacggagcc gatgccaata
    10441 ttaccaactt cctggttcaa aaagtgtttg acggaacaca taaaacggtt cttgattcag
    10501 ggttgaactc agcaggtttt acagtaactg agacatatta tcagggcgtg gaagaagagg
    10561 tagagtggac tttttcggtt atggacagga acagaaaatc agcttctgtg aaaatcaagt
    10621 tgataaaaga tccgaactca caatttggcg gaatactcga gtatgacagt attactgttg
    10681 gattgcaaag caatcctgat gtaggacatt tcttcctgcc atcgctgaac gaagtgtatt
    10741 tcgaggattc agctactatg tttcaggatt tggtcgatgt tctttgctat tttaattaca
    10801 gtgaggataa tggtgttaat cttccatcac caacattttc ctctcccgga gaagacccca
    10861 acgcttccgg ctatttgtac agtgaatatt atccatttat cagcagctgg gctacgcgaa
    10921 actacacgaa atacgatatc cgtgctgaca atggcgtgac cttgcaggaa ttcttttcgt
    10981 cgcacaacga cagccttctt attgtttcgt atgacgacgt atggggcaag aaaaaatata
    11041 aatgggctta tagcggcact ttgattcctt ttatgacagc aggaggtaaa aaaggcatta
    11101 tcagggtaat tgaggctgac accgtagcaa ctggaacaat tacgttttca atgaaaattc
    11161 aattataaat taacctttaa aaacaatgat tatgaaaaaa aatctattat ttgtttttgc
    11221 atttttgatg ttgtttagcc cgataatagt tgctcaacaa caagtgatga tcggatttac
    11281 cttttccgat aatactgacg ctgattttac tgctgataac ggagcagggc ttccaggaaa
    11341 cgacagctac aatgttcgtg cagaaaatgc agcaggagat acccgcgatc tgatttactc
    11401 cgcaggtgca acaacttacg cagctacagc cgataattgg gatgctggca gcgatgataa
    11461 attctggtcg ataaaattta aggcagacgg ataccagaat tttaaagttt catcgaaaca
    11521 gtattccgat cttacaggtc caaaggaatt taagctgcag tataaaatgg gtaacggtgg
    11581 cacatggaca gatattacag gaggtactgt tactgttgct aacgattggg caagcggtgt
    11641 tgtaaacgaa ataagcctcc ctgccgaact gaacgacccg ggtacaactt cgatatatat
    11701 tcgctggatt atgacctcta acacctctgt aagcaatgca acagttgaag caacgggtgc
    11761 ttcaaaaatc gacgacatta tggtatatgg ttcccctttg ccaacaccgg tttcaattat
    11821 cgggttcaat ttcaccgaca ataccgatga agaattcaat gccgatttag gccttgcagg
    11881 gaatctgacc tatgacatca gagccgaaaa tactgccggc gatacccgca cactaactta
    11941 cactgagggt gctaccgatt atgctgccac ggcaagtggt tgggatgctg gctcaggaga
    12001 taaattctgg tcaattaaat tcaaagccga cggataccac ggtatgaaag tatcatcaaa
    12061 gcagtattcc gaagccacag gtccaaagga ttttaaactt caggcacgtt tcagcggtgg
    12121 cgattgggta gatattgata atggaacttt aaccgttgcc agcgactggt cgacaggcgt
    12181 tgcagaagag cttgatttgc cggcaagttt cgataacccc ggaacgacat caatttatat
    12241 caggtggatt atgacttcaa atgataatgt cgaaaatgga actgttgatg ctgatggtat
    12301 atcgaagata gacgacatcc tggtaacagg ttttaactcc acaggttttg agtctgtgat
    12361 tttcgaaaat ttggtttctg tattccccaa tccatgcaat agccatgtga acatcgcagc
    12421 aaatgaagaa atagcatatt gctctttgta tgacatgcag ggtaaactga taagcacaac
    12481 gcgtgttgat gctgacgaaa ccacgatatc gaccagcaat ttggcaaaag ggatgtacat
    12541 tgtacgagtg tttttcggaa acgaaaagac tcccgttacg gaaagaatta ttgttgaata
    12601 attaaaatat catatcttgg cattgccggt agcattgaag ctttcggcaa tgctttttta
    12661 gggactttaa tgcgtaaaat actaacaata ttgttgttgc tgttgaatgt atattcattt
    12721 tcgcagacta tttcgggtta tgttaccgac agtttacgaa atatcatgcc caatgcaaac
    12781 atctcgttaa accgccacga aaagggcact atcaccgatt ctaacggtta ttttaaatta
    12841 tcactcgaaa aaccagggaa atacatactg aaagccagct ttataggata tgaacccgcg
    12901 ataaaacaaa ttgaactaaa tgacaatcag aatattacaa ttgattttgt gctgattccg
    12961 tccactattt ttacaccaga agtcgaaata caggcaaaga aaaccgatgg atttgaccat
    13021 tttgatgtgc cgattagagt taaaacatta aatacttacg atttggtgca aatacctgca
    13081 attagctcaa gtaagctatt taataatatt tccggaatag atgttagaaa cgaattcgga
    13141 attttttcgt cgcagactgt ggtatcattg cgcgggctgg gaggggggca atcggggact
    13201 ctggtagtga ttgacggaac acccttgaac aaatcggaat ccggctcggt aaactggaac
    13261 atgattgata ttgatggaat tgaaaaaatt gaaatcatga aaggaccggg ctctgtttta
    13321 tttggcagca acgctatggg aggcgtgata cttatcacca caagcaaacc caagtcgctg
    13381 ttttcagccg atatgagtct gggttacggg cgctacaata ctatggaaac caaacttaat
    13441 acttcgggga gaagcttaaa caacttgata tactggaaag ccaatatcat gttccggcgc
    13501 agtgatgggt atgtctacac tcccgattac gaaattattg aaaacgatac cattattgtc
    13561 ccggtttatc tgagagaata tagtgcagga gcaatgcttg gatttcagat aaacgaacaa
    13621 aaccgacttg aactctcatt cgattactac gacgaccgta ggggctccgg aataaaaata
    13681 tttgaggatt tgggagcaaa tgatgagcat gatacctata gtacgtattt acgttataag
    13741 ggcaattacc gtaaattctc tttatactca aacttatatt tgttgaatga aaactacggt
    13801 aagcttaacg agtattatag cgatggggaa tataaactat acgaagtaga ttcgaagaga
    13861 ctggattatg gagcaaaaat ttggactgaa atacctgtaa gcagtaaatg cgatattaca
    13921 gcaggtgtcg attttaaaac tggtgcagtg aatgcctcgg atatatactt tacctcgact
    13981 gatgttatca gaaatcgtgg agaaatgaat acactggcag cctaccttca gttgaaatac
    14041 tatattacac cgcactttac cgctatcgga ggtgttcgct acgatatagc tttgtttcat
    14101 gatgccgcat tcagtattga acagccatca tattctatcg aatatcttgc cgggtatcag
    14161 ttcagtgatg ttgaaacaga gcactggtca gcaacaaacc ctaaattcac tttgcaatat
    14221 acaattaatg aattcgataa aatttatgtt acggtggcca aaggatttgg agcaccaatt
    14281 ttagaagatt tatgccgcac gggaaggaaa aaaattggtt ttaaaattgc aaaccctgcc
    14341 ttgcaacccg agcatataac caatttcgaa acggggtttg actttgggtt gggcaagaag
    14401 ctgaattttg ctgcatcgtt ttattacacc ttaggcgata attttattta tttcgttact
    14461 acaggcgatt ctgtaaatat gggctatact attgcgccga tatatcaggc aaaaaacata
    14521 agtaaagttg aaatatttgg tgctgaagct gatataaatg tcgctcttac aaataatctt
    14581 aaggcttttg gaaactacac ctataatcat tctcagataa aagaatatat tgcaaatctg
    14641 gaacaaggcg ataaagacct gtcggggaag tttctgaccg acgtacctat gcataagttt
    14701 tccctgggct tgagttttca aaataaatat gtgaatatct cttcatcggt aaaatatacc
    14761 ggtgagcgat ggctgaacga tgaaaataaa tacgacaatg tatatctaat gtctgacaaa
    14821 taccccgatt attttcttgt tgacatgaaa ctatggaaac agatcaggtc tttgactctg
    14881 agtgtaaata tcgataacct gctgaacaca atatataccg acagcaaagg gtttaaatcc
    14941 cctggaattt tctggatgat tcaagtaaaa tataattttg ttacacctaa aactaaatag
    15001 ttatgaaaaa aatcaaagta gtttattatt tatctgtcat tctcattgta atggcatctt
    15061 gtgcagggaa tagcaatcgt ggaaaacagg aagttggcaa cagctcacag caaactgttc
    15121 aacatgacac ggcagattat acaaataatt tttatgacaa tgccgagaaa tacagtctca
    15181 aaacttacga tcttgaagtg gtaggtgaag tgtcaaaccc cggcaaggtg gatttctcaa
    15241 aactaccact gcgaagtgtt attgttaagg aaacgctcct tggcgacaat ggggataaat
    15301 ttgtaggtgc atattgctac gatgggtatt cacttttcga tatcctgaat gcatatcatg
    15361 tcgccaagaa aaacgaagaa gaatataagc cgattatcga catgtatgtt gaaattgaaa
    15421 atgccgatgg agaaaaggtg gttttcagtt ggggtgaaat atattaccca aaccatttgc
    15481 acgaaataat aatcgcaact aatgtaatgc gcattgtacc aagtaaaacc aatgaattat
    15541 gggaattacc caccgataac aaactggttg tagtaagcga tcttattact gaacgaaata
    15601 tcagtaaacc tgtaaaaata actgttaaat catacgataa gcaactggtt accataaaag
    15661 gcaagacccc gctttttgca ccaaagttcg attttttcaa ttccgataaa ctgatagcca
    15721 catatactca aaacccgaaa gacattgctg aattaacctt acataccata ttttatggcc
    15781 gcggcagagg cattcacagc acccaaccgt ttacgggtgt tccactgaaa caactaattg
    15841 ccggacacat tccgttgact cagacaagca tcaggcaggg attatttctt gctgctgcag
    15901 atgatggctt cagggcggtg tacacttata gtgagttatg caaccgcaac gatcaagcag
    15961 atgtgctatt aatttgccat ccggaagtaa ctaccgatgg tattttcaga ttgttcccaa
    16021 gttgcgattt cttcagcgac agagctatta aagctctgaa tgcactttat tattcagagg
    16081 caaaataaca ttctcacagt ttcgcttatc ctgaaaactc cttgctgtta gaaatcgttt
    16141 ttatcgcagg attaattaat tttgtggcgc attattacgg tctaatgaaa aatttttcag
    16201 catttacgct gactttatgg ttattgggag gaatggtttt gctttttatt gcagctccat
    16261 tgctgggttt attcctgtcg accggagcac caaaactctt tgatactatt caggatcgtg
    16321 aggttacccg aagtatttca cttactttgt gggcatcgat ggcagcgaca attatttttg
    16381 ccattggtgc tattccgctt gcatggttat tagcacgccg gaaattcaga ttcaaaggac
    16441 tggtaaatgc tcttatcgat ttacccattg taataccgca ttcagctgcc ggtatagcta
    16501 ttttaggttt agtttctcgt gatacacttc tagggcgcat aggagagagc atggggctaa
    16561 gttttgtaag caaccctgcc gggataatta ttgcaatggc ttttgtaagc ttacctttcc
    16621 ttattaatgc agccagggat ggcttttctg cagtacctga aaaactcgag aaagctgcct
    16681 ataacctggg tgcatcacca atgagggtgt tttttacgat aagtttgcct ttggcatgga
    16741 gaagcattgt ttccgggctg ataatgatgt ttgctcgtgg gatgagtgaa tttggggctg
    16801 tagtaatcgt ggcataccac cccatgacaa ctcccgtttt aatctacgaa cgctttggct
    16861 cttttggtct gtcgtatgca cagcctgttg ccgttatatt tattagtata tgcctgttgt
    16921 tttttgcatt gctgcgttat ttttcaaaca gaactgccct tgattgagct gaaaaatata
    16981 tcgaaaaaat tcaggaattt ttcgctgaaa agcattaacc tgcaggttga acagggtagt
    17041 tattttgttt tactgggaaa ttcaggctcg ggaaaatctg ttctgcttga aactattgca
    17101 ggcttctatt cacccgacag tggccaaata tttattaata aaaaagatgt aaccaatctg
    17161 tcgattgcaa aacgtaaaac agctctggtg tttcacgatt cagcactatt cccgcattta
    17221 accgttaggg gaaacctgat gtattcgtta aaaacaggca aaatacaaag aagtaagtgg
    17281 gattcccttg tgaacgatat ggcctctctg acagaggtat cacaattgct ggaccgctat
    17341 cctgcaaagc tgtcaggtgg cgagaaacag cgtgttgctc tcggcagggc attgttgcag
    17401 aaaccccatg tgctcatgct tgatgaaccg cttgcatcga tggatgttac attacgaagc
    17461 aacatgcgta gcttactgcg taaaatcaac agaagcgggc agacaataat acacgtaacg
    17521 cacgatttcg aagaagcagc tatgctggca actgaaattg ctgttatcga aaacggcagt
    17581 ataatgcagt gcgggaaacc agcacatgtt tttcaaagtc cgaaatcaga atttattgca
    17641 aggttttcag gctataaaaa tttctttgaa ggcaatattg ttaataataa tggtcatagc
    17701 gtatttcgta cgaaaaacat tgaaattgtg ctgagtgatt cagaggcagt tggatatggt
    17761 tttgctgcaa ttcgcagcga ggatgtctct attgggatcg agaagcccat ttcttcagca
    17821 agaaataatt acagaggtca ggttacctca attgaacagt atcattcaaa ttatgaaatt
    17881 attgttgatg caggtcagga attcagcgca tatattactg ctgaagcgat gaaggagttg
    17941 gcgcttgatg tgggttcatg ggtatgggta tcgtttaaag ctttttcagt taaatttatt
    18001 gaaaaatgat cgactataaa gaagctgcca taataattca aagagcagtt gaatctgtct
    18061 caaccgaaac aattccctat agcattgctc tgcacagaat tattgctcag gacattgttt
    18121 cggatgtaaa catgccccct ttcgacaagt cggcggttga tgggtatgca tgccgcagaa
    18181 gcgaccttgg aaataagctt atgttaaagg acgttatcag agcagggcag gagcaccaca
    18241 ttgttattga caaaaatgaa tgtgctaaaa taatgacagg ggcaatgatt ccagctggtg
    18301 ccgattgtgt gttcatggtc gagcagagtg aaatatcgga ggataaaatg attcgtttta
    18361 tcggtattaa aacgtctgat aatattgccc gttttggtga agatgttaaa acagggtcag
    18421 tggtgttacg gaaaggtact ttcattgaac caaagcacat ctcggtgttg gctgcaacag
    18481 gaactactat gatagaggtt tttagaaaaa taaaagtagc tataattacc acaggagatg
    18541 aaactgttga accagatatt a
//
LOCUS       DAOOQP010000055        18192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3739_length_18192_cov_5.439102, whole genome shotgun sequence.
ACCESSION   DAOOQP010000055 DAOOQP010000000
VERSION     DAOOQP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3739_length_18192_cov_5.439102"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2795
                     /locus_tag="PLK75_07220"
     CDS             <1..2795
                     /locus_tag="PLK75_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HOY39075.1"
                     /translation="GNFGGGGTQTEGGQSGLDPGSFGLGGSNMVSTSTVSGGGGGYYG
                     GGVGNPAGGGSGYIGGVYDAETTEGVKTGNGQVVIHWETSYVVQTTGLPTGSEFPAGT
                     TINTFVSTDASGNSSSCSFNVVVNDNEPPVISCPSSFAQSTDHDACTYTINNPVVVNS
                     KSYYIAPANLFNLWECATGIFFNEPGNCGFSWNDDQLGTATSVNLSFSIAAEYLGEPR
                     NTFLNGYAESSYNVSNSYGCSINTNADHIVSMNLDAAHYVVGGGNTFWVEENGMTWGL
                     QTDEILNGYISRIDVDYLKNADYDPVYSDNCPGAQITYVLTGATEATGSGSLEGVSIN
                     KGITQVEWTVTDATGNTASCSYQITVTDDQEPEITCPQNLSVSTDDGLCTASGVELGT
                     PIAFDNCNYTLSNNAVQPFSLGETVVTWTILDGSGHSATCQQTVTVTDNEIPEIECPQ
                     DVSVQTDAGLCTASGVDLGTSITSDNCGVFSVLNDAVEPYLLGHNIVIWTVNDVHGHS
                     VTCQQMVTVSDNEFPEIICPQNITVNTDDGSCTASGVELGIPSADDNCSYEVINDATE
                     PYSLGETLITWTVIDASDNTATCQQIVTVTDTELPEIICPQNIYVNSDAGACTASGVE
                     LGIPTASDNCNFTVSNNVLEPYVSGETTVIWTVTDGSDNTATCQQTVTVNDNEIPEIT
                     CPQDVSVHTDAGICTASGVDLGTPIASDNCGVFNVVNDAAEPYVTGQTVVTWTVTDVN
                     GNSASCQQMVTVIDNEMPVVVCPDDVVVANDYGQNYATGVELGEISAGDNCEIASIGN
                     NSPEYYPIGTTDVVWNIADIHGNTANCTQQVIVEDHTSVQYVKSDPIGLTIRPNPVQA
                     TAEIGVVLTENAVISLRIVDVSGKTVAILADDNAFSAGTHFFNYSVTLHGINPGMYYV
                     QLISGRATLSKKLVVY"
     gene            complement(2914..4416)
                     /locus_tag="PLK75_07225"
     CDS             complement(2914..4416)
                     /locus_tag="PLK75_07225"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39076.1"
                     /translation="MKTYVIVILLCSTVTSMAQISVNTHQGSIKNADTGKKTSVTCFK
                     HNSGNILRSKIKDGAEIYQLDSLVWLYYESGNWQNSSKEEYEFDDAGNLLTWITAYSD
                     YMGGAWQNSSREQYQYNDNNLVTEDISAYWDSEANDWENSSRLVYSYADNNELSNITS
                     SSWNTLINFWIPQYSNDYTYNLQGMVTQVIGQEWYYYTWRNYSRTDYEYNENIQEILS
                     TSYTWNDEWVFSEKYEKEYDDNGNLILNTSYYWNAESGEWGNSYRNELQYDESENLII
                     NTEFYWDSESNDWIGSSKTEFVYNKENQVAEKYVSYFDYYEHVWMYYDKYEYTYDFLG
                     NIISSIYSNTGNTIWEKDIEWTPNTKTDYEYNTAYELSDLFVPPSFEYSTMITVVNQS
                     IWNEEHWQDNYQGTYYYSLYVNTSIPNTTSHGLAVFPNPAADYIQMQWESEIPEYNLT
                     IYNAASVQVFSGEVYSLEKIDVSLLPAGKYIFKFNNKLYHSEIFKSVIIQ"
     gene            complement(4612..5235)
                     /locus_tag="PLK75_07230"
     CDS             complement(4612..5235)
                     /locus_tag="PLK75_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39077.1"
                     /translation="MIEIMPYTLADAAFLQSKQSGFIIWQPDATYVILGQSDTPETSL
                     NRDAIIADKIMVMKRPSGGHTVLLSPNTLIISVLDKSPDISQTRSWFNQISQNILIAL
                     KQCGVVNAEIKGTSDIVINNRKILGSSMYRSKDNVLWHGVLNVSEKPETIARYLAHPI
                     TEPKYRQNRNHISFVTSLSEQGYSLNSDLFADSFGRITGRVCLSCKQ"
     gene            complement(5294..6586)
                     /locus_tag="PLK75_07235"
     CDS             complement(5294..6586)
                     /locus_tag="PLK75_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY39078.1"
                     /translation="MKYNVIIIGAGPAGYVAAIRAGQLGMKTLVIEKAKPGGMCLNWG
                     CIPSKSLIESAKLFTRIKTANIHGVEGFDPESVQFNWQKAVQRAREVSSRLTAGVEFL
                     LKKNGVEFETGEAIITGQHTITVNNRNLESDNIIIATGSLPEKSTHRKSIGIQALYKL
                     EKLPKKIAVEGTGPVAVEFAQLFNMLGIEVFMQEPKTPLIPDADDYINDYLKNKLSKK
                     GIVFCQDGEAPENILWLNANRRKAVIPQSEIPLQQTEDGFIEANDFLMTSVEGIYAIG
                     DVNGKSYLAHAASAQGLFVINHIKGIKGDMHHDRYPMNVYSVPEIAQVGKTEQQLQAA
                     GIDYKLSSYPLSANAKAQIQNETEGIVRILSDKKYGEVLGVQIIAENATDMIAEAAAY
                     IEMEATVYNVANTIHAHPTISEVFMEAGFDAFDKAIHK"
     gene            complement(6659..7942)
                     /locus_tag="PLK75_07240"
     CDS             complement(6659..7942)
                     /locus_tag="PLK75_07240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOY39079.1"
                     /translation="MRYIFKFPDIGEGLDEGTILEWYVQKGQSVESSQSLVKMETDKV
                     VTDIPSPRAGVITAVYGKEGETIHVGDPLVEIELQGIEGAQAVEEAAKIESEIHAVAE
                     EGAGVVGTLEVAGNNAFLPSSDEGIETEPDNAHIKTKAIATPVARALARDMNIDINEV
                     KGTGPGGRVMKEDIEKHRTTVYTKSSASAPTIQEAKPVEIEPMSQLRKTIAKNMLNSK
                     HNAAHMTVFDDAEVNELVKIRSRFRDAYSEEGIKLSYLPFVIKAVALSLKKFKALNSE
                     MDMENSRIIYKKYYNIGIAVDTPDGLLVPVIKDADKKSIRQIAAEIHAISEKSRSRKL
                     GLDDIKDGTFTITSFGSIGGKYAVPVINYPQAAILGIGRLFEQPGFQGDQVIKNNMMP
                     LSLSVDHRIVDGGEVTRFLNQVIKYLNDPVTLIMD"
     gene            complement(7956..8954)
                     /locus_tag="PLK75_07245"
     CDS             complement(7956..8954)
                     /locus_tag="PLK75_07245"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOY39080.1"
                     /translation="MEGAQVMNKIMNMVNAVNDALDIMLQNDDRVIVYGEDVGVEGGV
                     FRVTEGLQQKYGVNRVFDSPLAESGLAGTAVGMAAAGLKPIIEMQFSGFAYPAFNQIV
                     SHVSRLHNRSRGKFKMPIVIRMPYGGGINALEHHSESLEAVWGHIPGLKVVIPSTPYD
                     AKGLLISAIESDDPIIFMEPKRVYRAIKQEVPEEKFSIPIGKAKIISQGTDITVVAFG
                     AMIREVQKAAALAKKENISVEIIDLRTIYPIDKETIAASIKKTGRIITVTEAPKSFGV
                     SAEISAIATEEAFLHLEAPPRRVTGFDTIVPLPRGEHHYMINPEKILYEIIRTVKF"
     gene            complement(8933..9976)
                     /gene="pdhA"
                     /locus_tag="PLK75_07250"
     CDS             complement(8933..9976)
                     /gene="pdhA"
                     /locus_tag="PLK75_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOY39081.1"
                     /translation="MDNIGKIINSKYFPKLSDTEVLKAYKDMLFARTADLMVVSYQRQ
                     GRIYTYPPNYGQEAIAGAVAQLVRNDDWVVPAFREMGLYLAKGATLKELFLYFMGYED
                     GSRFEKSNHMLPMSVPIASQLLHAVGIGYEINYRKKDEIVYTFVGDGGTSEGDFHEAL
                     NFAGVWNVPVIFIVQNNQYGISTPFRVQTASDGIAIKAMAYGIEGIQVDGNDFFAMYS
                     AVQHSIEHIKAGNGPVLIEAVTYRKGAHTTSDDPSKYRTKEEEEKWDATDPLKRLKSY
                     LTNKDVYNDADENKLIDEYKKEIDRQFTEAENYKPYPLEDAFKYMYVDMPDDLKHQQM
                     EQEKFLKWKELRS"
     gene            complement(10246..12927)
                     /locus_tag="PLK75_07255"
     CDS             complement(10246..12927)
                     /locus_tag="PLK75_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOY39082.1"
                     /translation="MKKRILFVIVFTSLAFCLTAQVKLIPTPQRIDYKNTSFRIYDKT
                     ALICTNVDLFYAEELSIFINQEMSLQLNNIDKPGKSSIELIRLKNDEELINVLNRNKL
                     DSEFRTGAEGYVLDISEKRIMIIALTDAGIFYGIQTLQQLIAANRTGESIPCLTIYDY
                     PDFAVRAWQDDISRGPIPTMELLKEQIRQMASYKLNYFYLYIEHVFKLENHTGIAPND
                     GITKEQIEELTAYASKYHVQLIGGYQSFGHMEKTLKLPEYQHLAENSHIISPAVPETY
                     KFLSDVYDEIVPVFNSAYFNINCDETFGLGEGKSKSMVDSMGIDGVYIYHINKLNELL
                     KKYNKKVLMWGDIVGSYPNSVKKLPEDITVIVWGYHAAESFDYAINPISSTGINFWVA
                     PGINCWSNIFPNFYETKINVYNFIRDGYKHKASGVLNTSWDDDGSNLFNNNWHGFIWG
                     AENSWKAPVCTSSTKESEKEMEARYNEFNIAYDGLFYGLNGGSITKVLVSFSRLHQSG
                     VRDILRNGNFFEPIFPIYNEYVSDEMREKNLDLLNQLEGFNDQILKLEPQIKRNNKTI
                     AYLKFAMKQVEFSLKKNLLRIDLYQYLNGSTIISDEVLKSKIIELINEAGLLKNEYAD
                     LWNKESRPDWLEVNLKKFDALISDLENLEGYCIISADDKITDQGRKITMRSLFGDLPV
                     YYSINEEAPDFSKKYTELLVIDYDANIKAHAVKDGKSFNATEYKILYHKGIGRLHKMY
                     SNCSKYHPSYDGGGKYALLDGKQGEPEFLRSGKWQGFSGQNIDIEIDLEKPQSFNSFS
                     MGFYQNIDTWVIFPKKIEIFVKNNIADEYVLLRTLVNEIGPEAKGELRQDFTAEFTNV
                     KTRYIRVVAYYYGKLPEWHHAGSGYESMIFADEIIIK"
     gene            13027..13167
                     /locus_tag="PLK75_07260"
     CDS             13027..13167
                     /locus_tag="PLK75_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39083.1"
                     /translation="MLSTGAKRKVNRSIGRGYGEMSSESPYIDLVCNEVGFNLSICNA
                     VI"
     gene            13306..13863
                     /locus_tag="PLK75_07265"
     CDS             13306..13863
                     /locus_tag="PLK75_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOY39084.1"
                     /translation="MSGDKYIIRNQNAVHFLTFTVIDWVDVFTRKEYKLELVDAMNYC
                     IKEKGLIVYAWVIMSNHIHCLWQASEGYNLSGIIRDYKKFTAKKIIKLIEEYPESRKV
                     WMLKKFAFAGKRLNRITKYKFWKDDNHAIEIDSFDTEVLLQKLDYIHNNPVKSMIVLE
                     PQDYVFSSAKDYCNEKGLVDIIVVH"
     gene            complement(13939..16206)
                     /locus_tag="PLK75_07270"
     CDS             complement(13939..16206)
                     /locus_tag="PLK75_07270"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOY39085.1"
                     /translation="MKTVLTFLLSFVALIGEAQNKESLIIVRKGKANYCIQAESPAAA
                     NAACILQQYVDSVSGVLLPISQKKQGKNNKISFVDYSELNAGTFHDSLRNDGFIISTL
                     GKNIYIYAKNAAGFRNAVYYFLEHQLSCRFFAVDAKVIPKNKEIEISPINTLQNPAFE
                     FRIDYNGDAFNKNFGEWHGLHNKPRNTNTGGFEVSEQWGLWVHTLHTLVPPEKWFDSH
                     PEYFALRNGVRVKDQLCLSNPDVLNITIESLREEMKKNPEALYWSVSQMDNFNYCMCD
                     KCKAIDEANGSPSGSLISFANAIAKEFPDKIISTLAYQYSRKAPLNVKPLPNVNIMLC
                     TIECDRNKPISQDSLEGSFTKDLKDWCALTDNVLIWDYVINFSNIIGPFPNFHVLKPN
                     LQLFQKNHVSMVFEQGWPHNSGEFTELRCYVLAKLLWDSSLNTDSLMQDFCHGYYGPG
                     GKYVYRYIQLSTQNLLASGKALTLYEPMSAHAQGFLSPENIDIYFDLFDDALMATQGN
                     KVYHHRIQMAMQPLRYAWLEVAKSLPFTDDWLFEKDEKGSYRVKEKARQYLRELCDTA
                     SIYGPILFHEISIKPQEYYERMSAYFENGVQMHKAVGKSISFVSPASPKYSANGLQTL
                     IDGVCGTDNYFSLWQGWYGEDIDATIDLGSEDSVSSIQLNCLINQASWIFAPESITVQ
                     VSNNGSEYSEIATYVNAEARNNTGKSIVPFIIEFPTSQNCRFLNIKLKNIGALPEWHG
                     SKGQTWIFVDEIIVR"
     gene            complement(16210..17547)
                     /locus_tag="PLK75_07275"
     CDS             complement(16210..17547)
                     /locus_tag="PLK75_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOY39086.1"
                     /translation="MQTMCKNIPVLILLFLVSGIGIQAQVSPPASDANKMQWFSDAKL
                     GIFIHWGIYSTGKTSESWEFYNGHISHKDYMAQINEFTAKNYKPEQWTALIKSSGAKY
                     VVITSKHHDGVALWNTKMNDLSIPKKSPAKKDVLTPFVNQLREDSLKVGLYYSLIDWT
                     YPDYPGFLRDSSKYKIENDTARWTHFLRFCHGQIAELLKAYNPDLVWFDGDWEHNAEE
                     WQAAKIRQMILDSNPRTIINGRLQGYGDYDTPEQSFPVTRPAMKNWELCMTTNDSWGY
                     YIGDNSYKTPYEIISIFADCISMGGNLLLDIGPREDGSIPEEQQFLLTTLGIWTSKHS
                     EAIFGSLGGLPPGYFYGPTTLSKDSCNLYLFLQGRQSGCIILKGITNDIKNIEVVGNK
                     TKLSHKVIGKMSWSDIPGLVYIDIPEGVQDEYMTVLKVTFDKPVSLYKAKGGL"
BASE COUNT         5109 a         3710 c         3615 g         5758 t
ORIGIN      
        1 ccggaaactt cggtggtggt ggaacacaaa ccgaaggcgg gcagtcggga ttggatccgg
       61 gaagctttgg tttgggcgga agcaatatgg ttagcacaag tactgtttct ggtggaggag
      121 gaggttatta cggcggaggt gttggtaacc cagctggagg cggttcaggt tatatcggcg
      181 gtgtttatga tgcagaaacc acagaaggtg ttaaaacagg caacggacaa gtggttattc
      241 actgggaaac tagttatgtg gtgcagacca ccggacttcc gacaggttca gaattccctg
      301 ctggtactac aattaatact tttgtgtcga ctgatgcatc gggaaactct tcttcatgta
      361 gttttaatgt ggttgttaac gataatgaac ctccagtgat atcatgtcct tccagttttg
      421 cacagagcac cgaccatgat gcatgcacct atacgattaa taatccggta gttgtaaatt
      481 caaaatccta ttatatcgca ccagctaatt tgtttaacct ttgggaatgc gcgacaggaa
      541 tcttttttaa tgaacctggc aattgcggat ttagttggaa tgatgatcag ttgggtacag
      601 ctacttcagt gaatctgtct ttcagtatag cggcggagta tcttggtgaa cctcgaaata
      661 cctttctaaa tggttatgcc gaaagtagtt ataacgtatc taactcatat ggttgttcaa
      721 taaatacaaa tgccgatcat attgtcagta tgaatcttga tgcagcacat tatgttgtgg
      781 gtggtggaaa tactttttgg gtagaagaaa atggcatgac ctggggtttg caaactgatg
      841 agattcttaa tgggtatatt tctcgtattg atgttgacta ccttaagaat gctgattacg
      901 accctgtgta ttccgataat tgccccgggg ctcaaattac ttatgtgctg acaggtgcta
      961 cagaagcaac tggatcgggt agcctcgaag gagttagcat aaacaaaggt attactcagg
     1021 ttgaatggac tgtgaccgat gcaacaggaa atactgcttc ctgcagctac cagattacgg
     1081 ttaccgatga tcaggaaccg gagattactt gtccacaaaa tctatcggta agtaccgacg
     1141 atgggctatg cactgcaagt ggtgtggaat taggaacccc gattgcattt gataattgta
     1201 actatacctt aagtaacaat gcagttcaac ccttttcgtt gggtgaaact gttgtaactt
     1261 ggacaatact tgacggttca gggcattcag ccacctgcca gcagacagtt accgttaccg
     1321 ataatgaaat tccggaaatt gaatgcccac aggatgtttc ggtacaaact gatgcagggt
     1381 tatgtacagc cagcggagta gatttaggaa caagtataac atcggataac tgcggagtgt
     1441 tttctgtgtt gaacgatgca gttgagccct atttattagg tcataacatt gtaatatgga
     1501 cggtaaatga tgtacatggg cattcagtca cctgtcagca aatggtaaca gtatccgata
     1561 atgaattccc tgaaattatc tgtccgcaaa acattacagt caacaccgat gatggttctt
     1621 gcacagcttc tggggtggaa cttggcattc cttctgcgga tgataattgc agttatgagg
     1681 taatcaacga tgcaacagag ccatattctt taggtgaaac gttaatcacc tggacggtaa
     1741 ttgatgcatc ggacaataca gccacatgtc agcaaattgt aacagtaacc gacacagagc
     1801 tccccgaaat tatctgcccg cagaatattt atgtaaacag tgatgccggt gcatgtactg
     1861 ccagcggtgt ggaattggga attcccacgg caagcgacaa ttgtaacttt acagtaagca
     1921 ataatgtgtt ggaaccctat gtctcaggcg aaaccaccgt aatctggaca gtgacagatg
     1981 ggtctgataa tacagccacc tgccagcaaa ctgttacagt taatgataat gaaattccgg
     2041 aaattacttg tcctcaggat gtttcggtac atactgatgc agggatatgc acggccagtg
     2101 gagtagattt aggaacgccc atagcatcgg ataactgcgg agtgtttaat gtggtaaacg
     2161 atgctgcaga gccctatgta accggccaga ccgtggtaac atggacagtt accgacgtta
     2221 atggcaattc agcctcttgt cagcaaatgg taacggtaat tgataatgaa atgccggttg
     2281 ttgtttgccc cgacgatgtg gttgttgcaa atgactacgg gcagaattat gcaactggag
     2341 ttgaactggg agagataagt gccggcgata attgcgaaat tgcttccatc ggtaacaatt
     2401 ctccggaata ttatcctata ggtacaactg atgttgtatg gaatatagct gatattcatg
     2461 gtaatacggc aaattgtacg cagcaggtta ttgtggaaga tcacacatcg gttcaatatg
     2521 ttaaatctga tcctatcggg ttgacaatac gccccaaccc tgtgcaggca actgctgaaa
     2581 ttggagtggt gcttacagaa aatgcggtta tttcgttgcg aattgttgat gtatcgggta
     2641 aaactgtggc tattcttgcc gatgacaatg cattttctgc aggaactcac ttctttaatt
     2701 attctgtaac attacatgga ataaatccgg gtatgtatta tgtgcaactt atatcgggca
     2761 gggcaacgct aagcaaaaag ctggttgtgt attaagataa aggtttataa acttcgacag
     2821 gccctctaca ttgtaatagt gtactccata atatctgggt cactcacacc ggacttggat
     2881 gcgaacaaaa cttaataaat tgcggaaaga tctttactga ataataaccg atttaaatat
     2941 ctcgctgtgg tagagcttat tgttgaattt gaaaatatac tttccggcag gtagtaaact
     3001 tacatcaatt ttttctaaac tatacacctc tcctgaaaac acttgtaccg aggcagcatt
     3061 gtagattgtc agattatatt caggtatttc cgattcccat tgcatttgaa tgtaatcagc
     3121 agcaggattc ggaaaaactg ccaacccatg tgaagtggta tttggaatag aagtgttcac
     3181 atacagtgag taataatagg ttccctgata attgtcttgc cagtgctctt cgttccagat
     3241 ggactgattt accactgtga tcattgtact atattcaaaa ctgggaggaa caaacaagtc
     3301 gctcagctcg tatgcggtgt tatactcata atcggttttt gtgtttggtg tccactcaat
     3361 atctttttcc cagatagtat taccagtatt ggaatagatc gaggatataa tattcccgag
     3421 aaaatcgtag gtatattcat atttatcata atacatccag acgtgctcgt aatagtcgaa
     3481 ataggaaaca tatttttcag caacctgatt ttctttatta tagacaaatt cggtttttga
     3541 actgccaatc caatcattac tttcgctgtc ccagtaaaat tcagtattta taataagatt
     3601 ttccgattca tcatattgca gttcgttgcg ataactgttt ccccattctc cggattctgc
     3661 attccagtaa tagctggtat tgagaatcaa atttccgttg tcgtcatatt ccttttcata
     3721 tttctccgaa aaaacccatt cgtcgttcca tgtgtagctt gtcgaaagta tttcctgaat
     3781 attctcgttg tattcgtagt cggttcgaga ataattgcgc catgtgtaat agtaccattc
     3841 ctgaccaatg acctgtgtta ccataccttg taaattatac gtgtaatcgt tggaatattg
     3901 aggaatccag aagtttatca gcgtattcca tgaagaggat gttatgtttg atagttcgtt
     3961 gttatcagca taggaataca ccaaacgact cgaattttcc cagtcatttg cttcgctatc
     4021 ccaataggca ctgatatctt cggtaaccag attgttatcg ttgtactgat attgttcgcg
     4081 cgaagaattc tgccatgcgc cgcccatata atcgctatat gcagtaatcc aggtcaacaa
     4141 gttacctgca tcatcgaatt cgtattcttc ttttgaactg ttttgccagt tccccgattc
     4201 gtaatataac cataccagtg aatcgagctg atatatctca gcaccgtcct ttattttgct
     4261 ccttaaaata tttcctgaat tatgcttaaa acatgttaca gaagtttttt taccagtgtc
     4321 tgcatttttt atactgccct gatgtgtgtt tacggatatc tgagccatac tggtaaccgt
     4381 ggaacaaagt agtatcacaa ttacataggt tttcataata atacgattac atggtttatc
     4441 aaaaatatgg gaaattttaa tatttattac attgcatgaa tcaagccaac aatagaatag
     4501 atttttctta tcggtgctga atgccttgat taatgcaata tgtagcttgc tttataaata
     4561 gattggatgc agtcaggatt tctaaaatgt agctaaatat ttcttaaaat attactgttt
     4621 gcagcttaaa catacccttc ctgtaattct tccaaagctg tcggcaaaca aatcggagtt
     4681 gagcgaatat ccttgttctg aaagcgaagt aacaaacgat atatggttcc tgttctgcct
     4741 gtatttcggt tctgtaatgg ggtgtgccag gtaacgggca atagtttcag gcttttccga
     4801 tacattaagc accccgtgcc acagcacgtt gtctttcgac ctatacatgc tgcttccaag
     4861 aattttacga ttatttatca ctatatcaga agtgcctttg atctccgcat taacaacacc
     4921 gcactgcttc aatgcgatca ggatattttg gctgatttga ttaaaccatg aacgtgtctg
     4981 agaaatatcc ggacttttat cgagaaccga aataataagt gtgttgggtg ataacaacac
     5041 cgtatgtcct ccactggggc gtttcataac cataatttta tcggcaatta tcgcatcccg
     5101 gttcagcgat gtttcggggg tatcgctttg ccccagaata acataagtcg catcaggctg
     5161 ccatattatg aaccccgatt gcttagattg caaaaatgct gcgtccgcta atgtatatgg
     5221 cattatttct atcatggtgc aacgaaaaag caatttctca tccgattaca ttctgatttt
     5281 acaaatgtag cacttatttg tgaatggctt tgtcgaaagc atcaaaacca gcttccataa
     5341 atacttccga aatggtcggg tgggcgtgta tcgtgttcgc gacattgtaa acagtggctt
     5401 ccatttcgat ataggcagct gcttcagcaa tcatatctgt ggcattttct gcaattatct
     5461 gaacacccaa tacttcaccg tattttttat ctgaaaggat tcttacaatt ccttcagttt
     5521 cgttctgtat ctgagccttg gcatttgccg acaacgggta actactcaat ttataatcaa
     5581 taccagcagc ctgaagttgt tgctcggttt tacctacttg ggcaatttct ggaacagaat
     5641 acacattcat cggatatcta tcgtggtgca tatcaccttt tatgcctttg atgtgattaa
     5701 tcacaaacaa accttgtgcc gatgctgcat gagccaggta gcttttaccg ttaacgtcgc
     5761 ctatggcata tataccctca accgaagtca tcagaaaatc atttgcctcg ataaatccat
     5821 cttcggtttg ctgtaaggga atttcgcttt ggggaattac cgctttcctt cgattggcat
     5881 tcagccaaag aatattttca ggagcttccc catcttggca aaaaacgatg ccttttttgc
     5941 taagcttgtt tttcaggtaa tcattaatat aatcatcagc atctggaatc aagggggttt
     6001 taggttcctg catgaaaacc tcaatgccaa gcatattgaa taattgcgca aattccacag
     6061 ctacggggcc ggttccctca acagcgattt ttttaggaag tttctcaagt ttgtacaacg
     6121 cctgaatccc gatacttttt ctatgggtgc ttttttccgg cagcgaccca gtggcaatga
     6181 ttatattatc agactcaagg ttgcggttat tcacagttat agtatgctgc cctgtgatta
     6241 tcgcttcacc ggtttcgaat tcaacaccgt tctttttcag caggaactca actccggctg
     6301 taagacggga ggaaacctcc ctggctcttt gcactgcttt ttgccagttg aattgtacgc
     6361 tttcaggatc gaacccctcc acaccatgga tgttggctgt ttttattctg gtgaacaatt
     6421 ttgcactttc aataagactt ttcgaaggga tgcaacccca attcagacac atccctccgg
     6481 gctttgcttt ttcgataaca agggttttca ttcccaactg cccggctctg atagcagcca
     6541 catagcctgc cggaccagcg ccaatgatta ttacattgta tttcatttta tattttttta
     6601 tgctatgatt cttcattttt gcaagttaaa ccacaatgat taatatctgt ccatgtaatt
     6661 aatccataat caatgtaacc ggatcgttca gatatttaat tacctgattc agaaaacgtg
     6721 tcacctcgcc gccatctaca atacggtggt ccaccgacaa cgacagaggc atcatgttat
     6781 ttttaatcac ctgatcaccc tgaaaccctg gttgttcaaa gagcctaccg ataccgagaa
     6841 tcgctgcctg cggataattg attacaggaa cggcatattt cccaccaatt gacccaaaac
     6901 tggtaatggt aaaagtacca tcttttatgt cgtcgagtcc aagtttgcgt gaccttgatt
     6961 tttcggagat ggcgtgtatt tcggctgcga tctgacgaat gctttttttg tcggcatctt
     7021 tgataaccgg caccagtaaa ccatcgggag tgtcgactgc aataccgatg ttgtaatatt
     7081 tcttgtaaat gatacgggaa ttttccatat ccatttcaga gtttagggct ttgaactttt
     7141 tcagcgataa agccacagct ttaattacaa atggcagata gctcagttta ataccttctt
     7201 cggagtaagc atcgcgaaaa cgactgcgta ttttcaccag ttcattcact tcagcatcat
     7261 cgaatacagt catgtgggct gcattgtgtt ttgaattgag catgttttta gctatcgtct
     7321 tgcgtagctg actcatgggc tcaatttcta caggttttgc ctcttgtatt gtcggagctg
     7381 aggcagagga ttttgtgtaa acagttgtcc tgtgtttttc gatgtcttcc ttcattaccc
     7441 tgccacctgg accggtacct tttacttcgt tgatatcgat attcatatcg cgtgctaatg
     7501 cacgggcaac cggggtagca atcgccttgg ttttaatgtg tgcattatcg ggctctgttt
     7561 cgataccttc gtcgctggaa ggtaaaaagg cattgttacc agcaacctcg agggtgccaa
     7621 ccactccggc tccttcttcg gctacagcat gaatttctga ttctattttt gcagcttctt
     7681 ctaccgcttg tgccccttcg ataccctgta attcgatttc gaccagcgga tcgcctacgt
     7741 gaatggtttc tccctctttc ccatacacag cagtgattac accggcacgg ggtgatggga
     7801 tatcggtaac tactttatct gtttccattt ttaccagcga ctgcgagctt tcaacgcttt
     7861 gtcctttctg cacataccac tccagaattg tgccttcatc gagcccttcg cctatgtcgg
     7921 gaaatttgaa aatatatctc attgttctat gttctttaaa attttacggt tctgataatt
     7981 tcgtagagaa ttttctcggg atttatcatg taatgatgtt cgccacgagg taggggaaca
     8041 atcgtatcga atcccgtaac tctcctcgga ggtgcctcta gatgcagaaa cgcttcttca
     8101 gtggctatgg ctgaaatttc agcggataca ccaaacgatt ttggcgcttc tgtaacagtg
     8161 ataatacgtc ctgttttttt gatgctggca gcaatggttt ctttatcaat cggatatatg
     8221 gttcgcaagt cgatgatctc caccgaaatg ttttcctttt ttgcaagcgc agcggctttc
     8281 tgcacctcac gtatcatggc accgaaggca actacagtga tgtcggtacc ttgtgatatg
     8341 atttttgctt tcccgatggg aatactgaat ttttcttcag gcacttcttg cttgatcgcc
     8401 ctgtaaaccc gtttgggttc cataaaaatt atcggatcgt ccgattcaat ggctgaaata
     8461 agcaatcctt tggcatcgta tggtgttgat ggtatcacca ctttaagccc gggaatatgt
     8521 ccccacactg cttcaagact ttcggaatgg tgctcgaggg cattgatacc gccaccataa
     8581 ggcatcctaa ttacaatggg cattttgaat tttccacggc tgcggttgtg caagcgagaa
     8641 acatgactta caatctgatt gaaagcaggg taggcaaacc ccgaaaactg catttctatt
     8701 atcggtttaa gtccggcagc agccattccg actgctgttc ctgcgagtcc cgattctgcc
     8761 aacggggaat caaaaacacg atttactccg tatttttgct gcaatccctc ggtaacccga
     8821 aatacacctc cttcaacacc cacatcttct ccataaacaa ttactctgtc atcgttttgc
     8881 agcataatat ccaacgcatc gttcactgcg ttgaccatgt tcattatttt attcatgacc
     8941 tgagctcctt ccattttaaa aatttttcct gttccatttg ctgatgcttt aaatcatcag
     9001 gcatatcaac atacatatat ttaaaggcat cttccaacgg atagggttta tagttttcag
     9061 cctcagtaaa ttgacgatcg atttcctttt tgtattcatc aatcagctta ttctcgtctg
     9121 catcattata tacgtcttta ttagtaaggt atgactttaa acgtttcagt gggtcggtag
     9181 catcccattt ttcttcttct tctttggtac ggtatttcga ggggtcgtca gaagtggtat
     9241 gcgctccttt ccggtaggtt actgcttcaa ttagcacagg accattaccc gctttaatat
     9301 gctcgatgct gtgttgaacg gctgaataca tcgcgaaaaa atcgttcccg tcaacctgaa
     9361 ttccttcaat gccatatgcc atggccttaa tggctattcc atcggaagcg gtttgtactc
     9421 tgaatggtgt gcttattcca tattggttgt tttgaacaat aaatataaca ggcacgttcc
     9481 agactcctgc aaaatttaga gcctcatgaa aatcaccttc agaggtaccg ccatcgccaa
     9541 cgaaagtgta cacaatttca tctttttttc gatagttaat ttcataacct atgccaacgg
     9601 catgcaacag ctgcgaagca atgggcaccg acatcggcag catatgattc gacttctcga
     9661 aacgagaacc gtcttcgtat cccatgaaat acaggaacag ctcttttagt gtggcaccct
     9721 tagccaggta caaccccatt tcgcgaaagg caggaactac ccagtcatcg ttacgtacaa
     9781 gctgtgccac agcacctgca atggcttctt gaccataatt cggaggatag gtatatattc
     9841 gcccctgacg ctgatatgaa acaaccataa ggtctgcagt acgtgcaaaa agcatgtctt
     9901 tgtatgcttt cagtacttca gtgtccgata atttgggaaa gtatttgctg ttgatgattt
     9961 tcccaatgtt atccatcacc ctaaattgct ttttctttgt tatatgatat tcttgaaaca
    10021 tattttcctg caaattaatt tctattataa ccggtaaata aaaaacttgt ttagaactat
    10081 tataaataaa tttaatttat gaaactcttg aagctttatt tgaacccgga cagtgcagga
    10141 gttaaaaaat attaactact agggttgtga aacgaaaaat tcaaacacgc ttcaacactc
    10201 ctctatgagt taaactccac tatgttttac caactatttt aaaagctatt ttatgataat
    10261 ctcatcagca aatatcattg actcgtagcc tgatcctgca tgatgccatt ccggtaattt
    10321 accgtaataa taggctacaa ctctgatata tcgggtttta acattcgtga attcggctgt
    10381 aaaatcctgc cttaattcgc ctttcgcttc cggcccaatt tcattaacca gagttcttaa
    10441 cagtacatat tcatcggcta tgttattttt aacaaaaatc tctattttct ttggaaatat
    10501 tacccatgta tctatgtttt gataaaagcc catcgaaaat gaattaaagg attgaggttt
    10561 ttctaaatca atctctatat caatattctg acccgaaaag ccttgccatt ttccacttct
    10621 taagaattct ggttctccct gctttccatc aagtaaagcg tatttcccac caccatcata
    10681 acttggatga tatttgctgc aattcgaata catcttgtgc aaacgtccta ttcctttatg
    10741 gtatagaatc ttatattcag tagcattaaa cgattttcca tctttaacag catgggcttt
    10801 gatatttgca tcatagtcta taaccaaaag ttcggtatat tttttggaga aatccggagc
    10861 ttcttcattt atcgagtaat aaactggcaa atcgccgaat agtgaacgca tagtaatctt
    10921 tcttccttga tctgtaattt tatcatccgc agaaataata caatacccct caaggttctc
    10981 caaatcactt attaaagcat caaatttttt caaattaact tcgagccaat cgggtctgct
    11041 ttctttattc cataaatcgg catattcatt ttttagcaat ccagcctcat taataagttc
    11101 aataattttc gattttaata cctcgtcaga aataattgtg ctgccattta aatattggta
    11161 taaatctatc ctgagcaaat tctttttcaa actaaactca acctgcttca tggcaaattt
    11221 aagataggcg atagtcttat tatttctttt tatttgtggt tccagcttta aaatttggtc
    11281 attaaaacct tcgagttgat ttaacaaatc aagattcttt tctctcattt catccgaaac
    11341 gtattcgtta taaattggaa aaatgggttc gaagaagttg ccatttctta gaatatctct
    11401 taccccggat tgatgcaaac gggaaaaact aaccaaaact tttgtaattg aaccgccatt
    11461 taatccgtaa aataacccgt cataggcaat attaaattca ttatatctgg cttccatttc
    11521 cttttctgat tcttttgttg agcttgtgca gacaggagct ttccaactgt tttcggcacc
    11581 ccatataaat ccgtgccagt tgttgttaaa aaggtttgat ccgtcatcgt cccagcttgt
    11641 attcagaacg cccgaggctt tatgtttata tccgtcgcga ataaaattgt aaacattaat
    11701 ttttgtttca taaaaattcg ggaatatatt cgaccagcaa ttaatcccgg gtgcaaccca
    11761 aaaattaatt cctgtagatg atattggatt aattgcatag tcaaaacttt cggctgcatg
    11821 atacccccag actataacgg taatatcctc aggcaatttt ttaactgagt tcgggtaact
    11881 gccaacaata tctccccaca tcagaacctt tttgttgtac ttcttaagta gttcatttag
    11941 tttgttgata tgataaatat acacgccatc aatccccatg ctatcaacca tgcttttcga
    12001 ttttccctca cccaggccaa aggtttcatc acagttgata ttaaaatacg cactgttaaa
    12061 taccggaacg atctcatcat aaacatcgct caaaaactta tatgtttcag gtactgcagg
    12121 agaaataata tgcgaatttt ctgctaagtg ttgatattcg ggaagtttca aggtcttttc
    12181 catgtgtccg aatgattgat aacctccaat caactgcaca tgatatttac ttgcatacgc
    12241 cgtaagttct tctatctgtt cttttgtgat accgtcgttc ggcgcaattc cagtatggtt
    12301 ttcaagttta aagacgtgtt cgatatataa ataaaaataa ttcaacttat acgatgccat
    12361 ttgccttatc tgttctttca gtagttccat tgtgggtatt ggcccacggc ttatatcatc
    12421 ctgccaggca cgaacagcaa aatcgggata gtcatagatt gttaaacaag gaatactttc
    12481 accggttcga ttagcagcaa tgagttgttg taaagtttga atgccataga agattccggc
    12541 atctgttagt gcaataatca taattctctt ttcggaaata tctaagacat atccctctgc
    12601 tccggttcta aattcagaat ctaatttatt cctatttaaa acattaatca attcttcatc
    12661 attttttagt ctgataagtt caatcgaact tttacccggc ttatcaatat tatttagctg
    12721 aagcgacatc tcctgattaa taaaaatcga aagttcttca gcataaaaca aatcgacatt
    12781 tgtgcatatt aaagcagttt tatcgtaaat cctaaagctg gtgtttttat aatcaattct
    12841 ttgtggtgtt ggaattagtt ttacctgtgc agtcaagcaa aatgcaagtg aagtaaagac
    12901 gataacaaac agaattcttt ttttcattac aatttcaagt aagtgtcgtt cggtaaagat
    12961 agaaaatttt catattgtta atttggttac ctgcatcccg acatcttttg caacaaactc
    13021 cctgtcatgc tgagtactgg agcgaagcgg aaggttaatc gaagcatagg caggggttac
    13081 ggagaaatgt cttcggaatc accgtatatc gacctcgttt gcaacgaggt cgggtttaat
    13141 ctaagcattt gcaatgcggt aatttaagtg cattaaaaat acaccggtaa ataagtcctc
    13201 gttgcaaacg aggactagtg catatatcga cctcgtttgc aacgaggtcg ggtctaatct
    13261 aagcatttgt aatgcgaatc aaaattaatt tcataatttt acgcaatgtc aggagataaa
    13321 tacataatca gaaatcaaaa tgcagtacat tttctaactt ttactgttat agactgggtt
    13381 gatgttttta caagaaaaga atataagctt gagcttgtcg atgcaatgaa ttactgcata
    13441 aaagaaaaag gtttaattgt atatgcatgg gtaataatga gtaatcatat tcattgttta
    13501 tggcaggcat cagaaggata taacttgtcg ggtataatta gggattataa aaaatttaca
    13561 gcaaaaaaga ttattaaact tattgaagaa tacccggaaa gtcgtaaagt atggatgctt
    13621 aaaaaatttg catttgcggg taaacgattg aaccgaatta caaaatataa attttggaaa
    13681 gatgataatc atgccattga gattgatagt tttgatacag aagtgcttct gcagaaatta
    13741 gattatattc ataacaaccc agttaaaagc atgattgttc tagagccgca agactacgtg
    13801 ttttcttcag caaaagatta ttgcaatgaa aaaggacttg ttgatattat tgtagtgcat
    13861 taaaaattta ttaataaata agtcctcgtt acaaacgagg actagtgtca tggacgtgtg
    13921 tatgcaaaaa atcccggatt atcttactat aatctcatcc acaaaaatcc atgtttgtcc
    13981 tttggatccg tgccattcgg gaagggctcc gatgtttttc agttttatat tcaggaaacg
    14041 acagttttgg gaggtgggaa attcaataat aaaaggaaca atcgactttc ccgtattatt
    14101 tcttgcctct gcattaacat aagtcgcaat ttcagaatat tcgctgccgt tatttgatac
    14161 ctgtaccgtg atgctttcgg gtgcaaatat ccagcttgcc tggttaatca ggcaattcag
    14221 ctgaatactg cttacggaat cttcagatcc cagatctatc gtggcatcta tgtcttcgcc
    14281 ataccatccc tgccacaacg aaaaataatt gtcagtgccg caaaccccgt ctatcagtgt
    14341 ttgcagacca ttggccgaat attttggaga agcgggtgaa acaaacgaaa tgcttttacc
    14401 aaccgctttg tgcatctgaa caccattctc aaaatacgct gacattcgtt cgtaatattc
    14461 ctgtggctta atgcttattt catgaaaaag tatgggacca tagatgcttg cagtatcgca
    14521 taattccctg aggtattgcc tggctttttc ttttacacga tatgatcctt tttcatcctt
    14581 ttcaaacagc cagtcatcgg taaacggaag cgattttgcg acctcgagcc aggcataacg
    14641 taagggctgc atggccattt gtatgcgatg atggtacact ttgttccctt gggtagccat
    14701 aagggcatca tcaaataaat caaaatagat atcaatgttt tcaggtgaca ggaaaccctg
    14761 tgcatgtgcc gacatgggtt cgtacagggt cagcgccttg cccgatgcta aaaggttctg
    14821 agtggaaagc tgaatgtaac ggtacacata ttttcctccg gggccataat agccatggca
    14881 gaaatcctgc atcaggctgt cggtgttaag cgatgaatcc cataacagtt ttgccagcac
    14941 ataacaccgc agctcagtga attcaccgct attatgaggc caaccctgtt caaaaaccat
    15001 ggaaacatga tttttttgga ataattgcag attgggcttc aggacgtgaa agttcgggaa
    15061 aggccctata atgttggaaa aattaattac ataatcccag attaacacat tatcggtaag
    15121 cgcacaccag tcttttaagt ctttggtaaa acttccttcg agagaatcct gactgattgg
    15181 cttattccgg tcgcattcga tggtgcaaag cataatgttt acattgggca aaggctttac
    15241 attgagtggt gcctttcgtg agtattgata cgccagcgtg gaaattattt tatcgggaaa
    15301 ctctttggca atggcatttg caaagcttat caacgatccg gatggagacc cgttagcttc
    15361 gtcgatggcc ttgcacttat cgcacataca ataattgaaa ttatccattt ggcttactga
    15421 ccaatagagt gcttcggggt ttttcttcat ttcttcacgc aacgattcta tagtgatatt
    15481 taagacatct ggattgctga ggcaaagctg atcttttaca cggactccgt tacgtagtgc
    15541 aaaatattcc gggtgcgaat caaaccattt ttcggggggc accagtgtat gcagcgtatg
    15601 tacccacaag ccccattgtt ccgaaacttc gaaacctccg gtattggtgt ttcgtggttt
    15661 gttatgcagc ccatgccatt caccgaaatt cttgttaaaa gcatctccgt tatagtcgat
    15721 ccggaattcg aaagcgggat tctgcagcgt gtttataggt gaaatttcta tttctttgtt
    15781 ctttgggatg accttcgcat cgactgcaaa aaagcggcag ctcagctgat gctccagaaa
    15841 ataatacaca gcattccgaa aaccggcagc attttttgca taaatgtaaa tgtttttacc
    15901 aagggtagaa atgataaaac catcgttgcg cagactgtcg tggaaggttc cggcattcaa
    15961 ttctgaataa tcaacaaaac tgattttgtt gtttttccct tgtttttttt gagatattgg
    16021 taaaagaact ccggaaaccg aatctacata ttgttgaaga atacaggccg cattggcagc
    16081 cgccggagat tcggcttgga tgcagtaatt ggcttttcct tttctgacaa taattaaaga
    16141 ttctttgttc tgtgcctcac cgataagggc aacaaacgaa agtaaaaatg ttaaaacagt
    16201 tttcattttt tacaagcctc ccttagcttt gtaaagcgac accggtttat cgaatgtgac
    16261 tttcagtacc gtcatatatt catcctgaac gccttcggga atatcaatat aaacaagacc
    16321 cggaatatcg ctccacgaca tttttccaat cactttatgt gagagtttcg ttttgttgcc
    16381 aaccacttca atgtttttaa tatcattcgt aattcctttg agaatgatgc atccgctttg
    16441 tctgccctgt agaaaaagat acaggttgca tgaatccttc gaaagggtag tgggtccgta
    16501 aaaatatccc gggggcaaac cacccagcga accgaaaatg gcttcgctgt gcttacttgt
    16561 ccagatgccg agtgttgtaa gcaggaattg ttgctcttca ggaatgctgc catcttcacg
    16621 tgggccgatg tcaagcagca ggtttccgcc catgctgatg cagtcggcaa agatgctgat
    16681 gatctcgtac ggtgttttat aggaattgtc tccgatgtag tatccccatg agtcgtttgt
    16741 ggtcatgcac aattcccagt ttttcattgc aggacgtgta accggaaaac tttgttcggg
    16801 tgtgtcgtaa tcgccgtacc cctgcaacct gccattgata atggttctgg gattactgtc
    16861 caggatcatc tgacggattt ttgcagcctg ccattcttcg gcattatgtt cccagtcgcc
    16921 atcgaaccaa accaggtcag ggttgtaggc tttcagaagt tccgcaatct ggccgtgaca
    16981 aaatcttaaa aaatgtgtcc atcgggctgt gtcgttttca atcttgtatt ttgaagaatc
    17041 ccttaaaaat cccggataat caggataagt ccagtcgatg agggagtagt acagacccac
    17101 tttcaggctg tcttcacgca actggtttac aaatggtgtc aatacatcct ttttggcagg
    17161 tgatttttta ggaatactca ggtcgttcat tttagtattc cacagagcca ctccatcgtg
    17221 atgtttagag gtaatgacca catattttgc acctgatgat ttgatgagtg cagtccattg
    17281 ttccggtttg taatttttgg ctgtaaattc gtttatctgc gccatataat ccttatggct
    17341 gatgtgcccg ttgtaaaatt cccaggattc cgaagttttt ccggtggaat aaattcccca
    17401 gtgaatgaat attcccagtt tggcatcgct gaaccattgc attttattgg cgtctgaggc
    17461 tggtggactg acctgagcct gtattccgat gcctgaaaca aggaataata agattaacac
    17521 cgggatgttt ttacacattg tttgcatttg acagtttttt taactcatac aaaaatacaa
    17581 ttatttcaat tcgggaaacc cggggttaat tccctatggg taacgatttg gttgctgtag
    17641 aaagattatg ctaaattgaa gtcgaaactt ttgtttttag tggcattttg caaataaatt
    17701 tgcaatgcca tgtaaatgaa aaatgaccct aatcgggtca tttttctgat ggttgcggag
    17761 agacaggctc cgtaaatagg tttatatgtt gctgataata agcggattac aatagcaaaa
    17821 aatccgctgg taactaaatg gtaacaaagt aaagaacgag tggcaaaaat cagtttgcat
    17881 cattttgctc tatttttcaa acccgcatca aatctacaaa aaagattaat acgatgcaaa
    17941 ttggacccag ggttcgggag aaattgggag taaatatcga aaaacgtaaa gcacagaata
    18001 tcagatatat accgattttg gaacaaaaaa atttagctca aattttttga aatttcgcca
    18061 aaatgtatgg ttttattata tatttaaaaa atgataggtg tatgggttgg atttttaatt
    18121 tttgttcacg aactttaaca cgaatacaat taaaactatt tggccatgaa aaagacctgt
    18181 attgtagctg tt
//
LOCUS       DAOOQP010000056        17893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3829_length_17893_cov_5.080446, whole genome shotgun sequence.
ACCESSION   DAOOQP010000056 DAOOQP010000000
VERSION     DAOOQP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3829_length_17893_cov_5.080446"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLK75_07280"
     CDS             <1..448
                     /locus_tag="PLK75_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY39087.1"
                     /translation="LLVGAVANQRPDLFAVSLPAVGVMDMLRYHLFTIGRNWSIDYGT
                     SADSKEMFEYLYKYSPLHNIKEGVEYPATLVTSADHDDRVVPAHSFKYIATLQASYKG
                     ERPMLIRIESKAGHGAGKPTDKIISEYADVYAFTFYNMKFTPLFEK"
     gene            508..1143
                     /locus_tag="PLK75_07285"
     CDS             508..1143
                     /locus_tag="PLK75_07285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOY39088.1"
                     /translation="MNLRFLVIEGNIGAGKTSLACKMASQFNAKLILERFADNPFLPR
                     FYENPERFAFPLEMSFLAERYNQLKTELSQYNLFNDVVVSDYYFNKSLIFSSNTLRDD
                     EYHLYRQLFDIIHSKLPKPDLYVYLHRSIEKLLQQIKMRGREYEQNIQPEYLRKIEQA
                     YFTFFRQQNDYPCVVIDIGDMDFMHDEISYGNLVETIINGSYENGMNTVVF"
     gene            1244..2953
                     /locus_tag="PLK75_07290"
     CDS             1244..2953
                     /locus_tag="PLK75_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39089.1"
                     /translation="MRKFVTISLVVAFVVAMSGCASLKKMIKNAEDIDYKVVPEVLEM
                     HGGKVEVKITGHFPEKYFSKKVEAEITPVLVYEGGEKALTTIYVQGEKIKENNQVIVY
                     KSGGEFAYTDKVDYVDGMKMSRLELRIHGKQGKKESDFEPEVIAYGVIATPALVDPYA
                     KVVSGKDNFIKDIAESKSAIINYDKNKTDLKKNEIKDTDITEMQEYIVTVDTAARKEF
                     KGVTVKSYASPEGTIERNTTVSTGRSKTAQDFLAKEFKKIKDAENKDFVKTDYVVEDW
                     DGFKKAVQESNLPDKDMILRVVQMNSDPVVREQEIRNMSKTFDELEKDIHPQLRRSEI
                     TINTMNIGYTDDELKAIWAKDPEKLVQEELFYTATLFTDNNTKLAIYTKYTDKYDKDW
                     RGFNNLGVVQYEMNNISGAKTSLEKAKALQPNATVFNNLATVSLVEGDIAKAKEYYGS
                     ATGISEASYGQGIISITEGNYKAATEYFGSDCSFDAGLAKLLNKDYDGAIQAASCGAD
                     KDNAMNYYLKAIANARKGVKDEMFNNLRTATAKNASLKTKAKNDMEFYKFFEDPTFIE
                     IVK"
     gene            3133..4131
                     /gene="obgE"
                     /locus_tag="PLK75_07295"
     CDS             3133..4131
                     /gene="obgE"
                     /locus_tag="PLK75_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOY39090.1"
                     /translation="MSNENFIDYVKIFCRSGNGGSGSRHFRREKFVPNGGPDGGDGGR
                     GGHIILKGSRHMWTLLHLYYQKHVFAGNGSPGLGARKTGADGEDRIIEVPLGTVAIDA
                     ETGEKLLEITEDEQQLILFEGGKGGLGNDNFKSATNQAPDYAQPGIPGTEAWIILELK
                     VLADVGLVGFPNAGKSTLLSVLSAAKPKIADYPFTTLTPNLGIVSYYDYRSFVMADIP
                     GIIEGAAEGKGLGIRFLRHIERNSVLLFTIPCDSDDIGKEYKILVNELKKYNPELLIK
                     QRLLAITKCDLIDDEIQQWIKKSLPKNVPYVFISSVNGTGLSGLKDLLWTTINNPD"
     gene            4133..4723
                     /locus_tag="PLK75_07300"
     CDS             4133..4723
                     /locus_tag="PLK75_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOY39091.1"
                     /translation="MLQRLENIDQQLFLFFNALHSPTFDSLMFFITGSWFWLPFIIYL
                     IWMCYRRFSKWFWLAFLILAFCFLCTDQISGFIKNAVERYRPSHNLSLTGQVHLLNGY
                     TGGLYGFVSSHAANSFGLAVLAGLLFRNSLVTAIMLAWAILVSYSRIYAGVHYPSDII
                     AGAITGIFIAWIIYFAICRIPWFVKKLNLRNPASQV"
     gene            complement(4743..6515)
                     /locus_tag="PLK75_07305"
     CDS             complement(4743..6515)
                     /locus_tag="PLK75_07305"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOY39092.1"
                     /translation="MAGKTFVEKVLEANTGAIVFRKPHIVLTHDNTESIKSTFNKMGG
                     TKVHDPNQLLVVLDHNAPPTSAKLATSYQVIRNFVKEQGIKKFHDAGDGICHQLVANY
                     AEPGMIIVGSDSHTCTAGAFNALAAGIDRTESAGLYLKGETWFLVPESIKITLKGKLQ
                     KGVYAKDISLWIIGMIGSDGANYMSIEYHGEGVKTLSISERMTLANLASEMGAKNAVF
                     PADEVLEKHLGKKLNGVWADADAKYSKSIEIDLSKIFPLVAAPHNVDNVKAVSEVEGT
                     QLNQGLIGTCTNGRLDDIRTAAEILDGKTVAAGFQLLVIPASKAIYLEAMKAGYVEKL
                     MLAGANVLSSSCGPCLGTGQGIPGDGFKVISTANRNFLGRMGNEKSEIFLASPATVAW
                     SAIHGKITDPRNIQAGDKFPYQSPQSATVSVDPSDNRRLGNVWNYKDTDNLNTDQMFA
                     GNLTYNVLSSDAAAIMPHLFAGFDVNFAKNVQKGDIIICGDNFGCGSSREHPSVGLAH
                     AEVKAVIVKSVNRIFYRSSINQGLALIVLPEVVDAYKPGDSVDVIFEKGLVLINGKEY
                     HFEPLPDKLMGIIEKKGLVNWIKG"
     gene            complement(6644..8902)
                     /locus_tag="PLK75_07310"
     CDS             complement(6644..8902)
                     /locus_tag="PLK75_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOY39093.1"
                     /translation="MVNETHDNPKTEMDQERQSVVPQESLPQKPPKKRKRRTGLKIFI
                     VLVLLLVVLLFFLSDIAKWYIENNGKSLVNRNISISELHINYLKFSVSVKGFTLYEQN
                     EKDSFVSFDELRIDFDPWQLLSNRYAFSEIKLVKPNISVIYDEKGFNFDDIIAFFDTS
                     DEPDSSDEVVKYLVKNLNITEGFIRYEDKTIASVTELKNLGITIPEIAWDNSRSELGI
                     DFEMGENGKVSLSGAFDQEKGLYELHAKTEQLDASLLKEYLTDIVDVGSMGGALYTDL
                     SITGSIDNLMNIIVKGEAGVQNLLLTGPDMKPFATVQDIHVIIDSLNLLRENYCINSI
                     EINSPIISAVLAKDGANYDKVLAPFYADTLPDPDDTIEMHYNIDKLSITNGTVSYHDY
                     TLNRPFIMDISDIEFRMDNYTDLATKVPLDFKMLINETGKISGNGTVNMLTLDGLDMV
                     LSVKNMDMVFLSPYAEYFLARPITKGVMNYDCSVYMTPVALENNNHIKIVDLDMGKKT
                     KDTTAYKVPIGLALYILKDRNDVIEFDLPVTGNPSEPSFKLRKIIWKTLEEFMIKTAA
                     APFNAIGNAFGGNPEKFKQIEFSMLQDSLSEEQSANLSKIATMMKKKDKLAFEFIQTT
                     DPDLEKQLLAVRECKTQYLRNQKPSEDFGIIRKNAASMPWNDTGFLSYMGLTPETDEN
                     SVKGACMQKIGNDKAQLMLNQLLTLRENLIRKYFVDNNVPIEAYTIKNIDFRNMPDEM
                     KTPKFIINVTLR"
     gene            complement(9002..10267)
                     /locus_tag="PLK75_07315"
     CDS             complement(9002..10267)
                     /locus_tag="PLK75_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOY39094.1"
                     /translation="MKLFRVSILFLLLCLSSIVAKPAFILLPMDESQKNHLKAYGITY
                     WILQNQIEAWWLLNYRGGSFLMPFAKEIESECNIRGVSYEIIADAQKNAIFNEISQPD
                     VNMDAVKLEKAPKIAVYSPPNKLPWDDAVTLALTYAEIPYDIVYDEEIVNGELSKYDW
                     LHLHHEDFTGQYGKFYGSYRHMQWYQNQQNEMEAMAARLGFNKVSQLKLFVAKTIKAY
                     VANGGFMFAMCSATDTYDIALASDGVDICAQMFDGDAADPVAQQKLDFSKGFAFRDYT
                     LIQNPLEYEHSNIDVSNTRKMSQEQDYFTLFEFSAKWDPVPTMLCQNHNNMIHSFMGQ
                     TTAFDSRFVKPDVLVMGENKSVGEVKYLHGEYGKGMWTYYGGHDPEDYRHYVGDQKTD
                     LSLYPNSPGYRLILNNVLFPAAKKKKLKT"
     gene            complement(10383..10955)
                     /locus_tag="PLK75_07320"
     CDS             complement(10383..10955)
                     /locus_tag="PLK75_07320"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOY39095.1"
                     /translation="MSSEWFNYLILPLLIFTARIFDVSIGTIRIIMVSRGKKLIAPLL
                     GFFEVLIWIIAVSKIMEHLDNWVNFIFYAAGFAAGNYIGMILEEKLAMGTVGVRIVTA
                     RSGMQLIQTLKEKGYGLTFMNAQGREGEVNVIFLTVKRSSLKELIPLIQNFNPNAFYT
                     IEDIRYVSSGYSNSAAGSRSRTGMLWRKGK"
     gene            11129..15616
                     /locus_tag="PLK75_07325"
     CDS             11129..15616
                     /locus_tag="PLK75_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY39096.1"
                     /translation="MKLHGLIAAVLLLTANAFAQQGFYAGFSQPHENVYEISFDLADW
                     SLNQVTHDGVIYQTIAMGTSAVCDKKGWAELPVISAAIQLPPEKDVDIAVAEASYYDI
                     QLQHPMLPSRGIIARSEDPSTIPYWTDPASLVDEFYPQQHIGMDEPFIFRDVRGTAVR
                     VIPFRYNAATGVLRVYTRIVVQLTENNHTPTNPLIVDNTNRILEVEGIYRSMFLNYSP
                     SATKTDLAMDEYGDLLVLVPTSYASTITPYVTWKREMGYNVTVTTVSTGTNVTSTIQT
                     AYNNNPNLLYVQLVGDWADIKSNTLTSSVVSSSYNVGAPMDPQMGCVSGTDNFADIAI
                     GRFSCANAADLTVQINKAIKYEKEPDNVAGWRETFIGVGSEEGTGDDSEYDYQHIQRI
                     YTQRLDGFTYDTHRQNYQSGANATTLAGHINTGGSTIAYCGHGDYTQWVTASFYNTNV
                     NNLSNGDKLPFVVSVACLVGAFHNSSVVFAEAWLRKSGGGAVVGWFATISQPWNPPMR
                     GQDYFYDILIGGFNYSSYSGQSGLTTSEQRTHFGSIVVNAMNLMLTESNTSQDTETTK
                     TWATFGDASLQLRTKQPVALVLSNTSPAVGTPFAGTAYIGSTPAANVLICISQNGSYY
                     HGLTNSSGAYSITHSLTQGDALLVATAFNTTTIYQTVTCVGGTPCWAVNTLSGTSDGS
                     TVNLTWSAPSEGSVSGYNIYRDGVLQSTTTSTTYTQSSVPNGTYEYCVAALFNNEECY
                     LRECVTVVVNDGSNNDCDSPSDLGIAEVNSTTHTLTWIEPEGAGNIAEDVESHTSWTL
                     NSPGSVGWSYIDGDALATFGASAFNFTNESSAMAYIVFDHAGTTGGTPNGSAHSGNKV
                     FADISPSNSSAANDWIISPELNFDEAFTFSFYAKSYVSTYGLERFKVAYSTTGTQQAN
                     FTFVTTNYIEAPVTWTLYTYTIPATAKYVAIQCVSADAFIFMLDDLYIGDGGGVAEAP
                     IGYNVYCDNVFLGNAAGTNFTNSSATTGYHQYCVEAVYADGCISPEICATIGSTATYT
                     IAATAGANGTISPAGTTTVSQGGSQTYTITPNACYHIADVLVDGVSVGAVTSYTFTNI
                     NANHTIAATFSATTYAITASAGSGGSISPSGGTAVPCNGSQTYTISPNACYQIANVLV
                     DGTSVGAVTTYTFNNVNANHTISVSFSVITYSINASAGTGGSITPSGNTSVTCNGSQT
                     FTITPSSCYQIDDVLVDGTSIGAVTNYTFNNINANHTISVSFELLTYTITASAGAGGS
                     ISPSGSVDVNCGSGQTFEIAPENGYEVASVLVDGNDVGAVSSYTYSNLTSNSTIVANF
                     SLITVEECSAPENLSATATADYIVLNWDAAPNAISYNVYRNGALLVNTTSVQFFDVTA
                     EEEVEYSYYVVSVCSDSESDPSNPASATINVGIIESNDNTITAYPNPTSGIVEIVLKN
                     DSDNYHGLVLTSLDGKMLLNSVIEGETIRIDLSDFADGMYIIRISGSSGNDKILHIVK
                     Q"
     gene            complement(15689..16138)
                     /locus_tag="PLK75_07330"
     CDS             complement(15689..16138)
                     /locus_tag="PLK75_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39097.1"
                     /translation="MKKLILTVSATLLLNAVIQAQEYKVITSVESIVPMGVGRSRIIE
                     HQEKIDAGAFTTYREQGTDTKQSEVSRDEIKIDKFEETKLLNFYSAVGINFRNIASND
                     AVISAKINQMIADGWELAFVVTGVESHGSKDDDNGIFITRYIFKRQN"
     gene            complement(16174..16995)
                     /gene="cysQ"
                     /locus_tag="PLK75_07335"
     CDS             complement(16174..16995)
                     /gene="cysQ"
                     /locus_tag="PLK75_07335"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOY39098.1"
                     /translation="MVIGNDALISLMHDFAIPAAIEAGKTCMKFFGSVYHVEYKQDLS
                     PLSEADMAANTIIAQLLKKTRIPIISEESSQISYTERIRWETFWLIDPIDGTKEFIKG
                     GNDFTVNIALIHNRVPVLGVIFAPALNTIYFGYATANAFRALVHSNNELTSIVKLPSE
                     KPELVTIVSSKSHKNIATEMFTEKIRNTFQDTKNVSIGSSLKLCMIAEGTASLYPRMG
                     SINEWDIAAGHALVRASGGHVINISTTSEVLYNTESLTTPDFIACLDPVLLFQVI"
     gene            complement(16989..17762)
                     /locus_tag="PLK75_07340"
     CDS             complement(16989..17762)
                     /locus_tag="PLK75_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY39099.1"
                     /translation="MNKIVLITGATAGIGKATAELLGKNGYRIIITGRRQSKLSELQD
                     TLKLKHNCSIISLCFDIRNRKEVDNAIDSLPTEWKDISILVNNAGLAAGFSPFQDCEI
                     SDWERMIDTNVKGLLYISKKVIPLIIAQGSGHIVNIGSIAGREVYPNGNVYNATKHAV
                     DALSKAMRIDLLKHNIKVTQIAPGLAETEFSEVRFHGDKQRAKEVYKGFEPLHAEDIA
                     DAIHYAITRPVHVNINDMLIVPSAQANAYNINKIIEEEW"
BASE COUNT         5138 a         3743 c         3677 g         5335 t
ORIGIN      
        1 acttctcgtt ggtgccgtgg caaatcaacg ccctgacctg tttgcagtat ctttaccagc
       61 cgttggagtg atggatatgc tgcgctacca tctgttcaca attggcagaa actggtctat
      121 cgattacgga actagcgctg atagcaagga aatgttcgaa tacctgtata aatattcacc
      181 cctgcataat attaaagagg gagtagagta ccctgcaact ttggttactt ctgccgacca
      241 cgatgacagg gtcgttccgg cacattcgtt taaatacatt gccacattgc aggcaagcta
      301 caaaggcgaa cgacctatgc ttatcaggat tgagtcgaaa gccgggcatg gtgcaggaaa
      361 accaaccgat aaaattattt cagaatatgc cgatgtgtac gcttttacct tttataacat
      421 gaaattcact ccgcttttcg aaaaataata cgtgatatta catttaaaaa tcctgctcat
      481 gcaggatttt tttttatttt taactccatg aatttacgat ttctggtgat agaaggaaat
      541 attggtgccg gtaaaaccag cctggcttgt aaaatggcat cacagtttaa tgcaaagctc
      601 atactcgagc gctttgctga taacccgttt ttacctcggt tttatgaaaa ccccgaacgt
      661 tttgcttttc cgcttgagat gtcgttccta gctgaacgct acaatcaact gaagacagaa
      721 ctatcgcagt acaatctttt caacgacgtt gttgtcagcg attattattt caataaatcg
      781 ctgatatttt caagtaatac actacgtgat gatgagtacc atctctacag gcagttgttc
      841 gacattatac acagtaaact accaaaaccc gatttgtatg tttatctgca ccgaagcatt
      901 gagaaattgc tgcagcaaat taaaatgaga ggacgggaat atgaacagaa catccagccg
      961 gaatatttga gaaaaataga acaggcatat tttacttttt tcaggcagca aaacgattat
     1021 ccctgtgttg taatcgatat tggtgatatg gattttatgc atgacgaaat ttcgtatggc
     1081 aatcttgtgg aaaccataat caatggaagc tatgaaaatg gtatgaatac agtggtattt
     1141 taattgtaaa aaactgcttt atttattggt gttttatgaa aacattatta tatttgcatg
     1201 ttagtttaat taaaaaagaa caataaactt aaaacaatac attatgagaa aatttgttac
     1261 aatttctttg gtcgtagcat ttgttgtagc tatgtcggga tgcgcaagtc ttaaaaagat
     1321 gattaaaaat gccgaagaca tcgactacaa agtagttcct gaagtactcg aaatgcatgg
     1381 cggaaaagtt gaggttaaaa taaccggtca tttccctgag aagtatttta gcaaaaaagt
     1441 agaagccgaa atcactccgg tactagtgta tgaaggcgga gaaaaagctc tcactactat
     1501 ctatgttcag ggtgagaaaa ttaaagaaaa taatcaggtg attgtttaca aatccggtgg
     1561 agaatttgct tataccgata aagtggatta tgttgacgga atgaaaatgt cgcgtcttga
     1621 gcttcgtatt cacggaaagc agggcaaaaa agaatcggat ttcgaacctg aggttattgc
     1681 ttatggcgta attgctaccc cagcactcgt tgatccatac gcaaaagttg tttccggaaa
     1741 agataatttt attaaagata ttgctgaaag caaatctgca atcatcaatt acgataaaaa
     1801 caagaccgac ctaaaaaaga acgaaattaa agatactgat attactgaaa tgcaggaata
     1861 tatagttact gttgatacgg ctgcccgcaa agaattcaaa ggcgttaccg taaaatccta
     1921 tgcttcaccc gaaggaacca tcgaaagaaa cactacggtt tcaacaggac gttcaaaaac
     1981 cgctcaggat tttttggcaa aagaattcaa gaaaattaaa gatgctgaga ataaagactt
     2041 tgtaaaaacc gactatgtag ttgaagattg ggatggtttc aaaaaagcag ttcaggaatc
     2101 gaatctcccc gataaagata tgatcttacg cgttgttcaa atgaattctg acccagtggt
     2161 tcgtgaacag gaaatccgca atatgtcaaa aactttcgat gaactagaaa aggacattca
     2221 cccacaactt cgtcgttccg aaattacgat caatactatg aatatcggat ataccgatga
     2281 tgagttgaag gctatttggg caaaagaccc ggaaaaattg gttcaggaag aactattcta
     2341 cactgcaaca ctgttcaccg ataacaatac aaagttggct atttacacca aatacaccga
     2401 taaatacgat aaagactgga ggggtttcaa taatcttggt gttgttcagt acgaaatgaa
     2461 caacataagt ggtgcaaaaa catctctcga aaaggcaaaa gctctacagc ccaatgcaac
     2521 cgtatttaac aaccttgcaa cagtttctct tgttgagggc gacattgcaa aagcaaaaga
     2581 atattatggt tctgcaacag gtatttctga agccagctat ggtcagggaa ttataagtat
     2641 tacagaaggc aactacaaag cagctacaga atattttggt agtgattgtt cattcgatgc
     2701 cggtttggca aagctcctca acaaagatta tgatggagca atacaagcag caagttgtgg
     2761 tgccgataaa gacaatgcaa tgaattatta tctgaaagcc attgctaacg cacgtaaagg
     2821 cgttaaagat gaaatgttta ataacctgcg tacagctaca gctaaaaatg caagccttaa
     2881 gactaaagca aagaatgata tggaattcta taaattcttc gaagatccta cattcattga
     2941 aatcgtgaag taatttttaa cagtataaat tcttgtaaag gcggcaattt gtcgccttta
     3001 ttttttttaa ctatgtatgc ttattcaaaa gccagggccg atgattattt cagcatttta
     3061 tttatgtttg caaataatac gcactcgtgt ccgttactct gaatattgac cggatgcata
     3121 aaacgacctg atatgagcaa tgaaaatttt atcgattatg taaaaatatt ttgccgttcg
     3181 ggcaacggtg gctcaggatc acgtcatttc agacgcgaaa aatttgttcc caatggtggc
     3241 cccgatggtg gtgatggtgg caggggaggg catataatcc tgaaagggag ccgccacatg
     3301 tggacattat tacacttata ttaccagaaa catgttttcg ctggcaacgg atcaccgggc
     3361 ctcggagctc gaaaaaccgg tgccgacgga gaagaccgta ttattgaggt accactaggc
     3421 acagtagcta tcgatgctga aaccggtgaa aaactccttg agataaccga agacgagcag
     3481 caattgattc tttttgaagg gggcaaagga ggtcttggca acgataactt caagtcggct
     3541 accaatcagg cacccgatta cgcccaaccc ggaattcccg gcaccgaagc gtggataata
     3601 cttgagctta aggtgttggc tgatgttgga ctcgtagggt tcccgaatgc gggaaaatca
     3661 acgctgctgt cggtgctttc agctgcgaaa ccaaagattg ccgattaccc attcacgacc
     3721 ttgacaccaa atttgggcat tgtgtcatac tatgattacc gatcatttgt aatggccgat
     3781 atccccggga ttatcgaagg cgccgccgaa ggtaaaggac tcggaatccg ctttctacga
     3841 catattgaaa gaaattcggt attgctgttc accataccat gcgacagcga cgacatagga
     3901 aaggaataca agattctcgt taacgaacta aaaaagtaca atccggaatt gctgattaag
     3961 caacgccttc ttgcaataac caaatgcgat ctgattgatg atgaaataca acaatggatt
     4021 aaaaaatcgc ttcccaaaaa tgttccctat gtctttattt cctcggttaa cggaaccggg
     4081 ttatccggac tgaaagacct tctttggact accattaaca accctgactg atatgttaca
     4141 gcggctcgaa aatatcgacc aacaactctt cctgtttttt aatgcgttgc attcacctac
     4201 tttcgatagc ctgatgttct ttataaccgg aagttggttt tggctgccat ttataatata
     4261 cttaatatgg atgtgttacc gacgatttag taaatggttc tggcttgctt ttttaatcct
     4321 ggcattttgc tttttatgca ccgaccagat ctcaggtttc attaaaaatg cagttgaacg
     4381 ttacagacct tctcataatt tatctttaac aggtcaggtg catttgttga atggctatac
     4441 tggagggttg tacggttttg tatcatcaca cgctgcaaat agctttggtc tggctgtttt
     4501 ggccggcctg ttgtttcgaa atagtcttgt tacagccatt atgcttgcat gggcaatatt
     4561 ggtgagctat agccgaatat atgcaggtgt tcattaccct tccgatataa tagccggagc
     4621 aataacaggt attttcattg cttggataat ctatttcgct atttgcagaa taccatggtt
     4681 tgtaaaaaaa ttaaacctga gaaaccctgc ttctcaggtt taaaaatgta ttctttttgc
     4741 ttttagcctt taatccagtt aaccaaacct ttcttctcga tgatacccat cagtttgtcg
     4801 ggaagtggtt caaaatgata ttctttacca ttaatcagaa ccagaccttt ttcgaaaata
     4861 acatctacgc tgtcgccggg cttatatgca tcaaccactt cgggaagaac aataagtgcc
     4921 aaaccctggt taattgagct acggtagaaa atacggttaa ccgatttcac aataactgct
     4981 ttcacttcag cgtgagccag gccaacagat gggtgttcac gcgaagaacc acagccaaag
     5041 ttatcgccac agataatgat gtcgcctttt tgcacatttt tggcaaaatt cacatcgaaa
     5101 cctgcaaaga ggtgaggcat aatggcagcg gcatcagagc tgagtacgtt gtatgtgaga
     5161 ttgcctgcaa acatctgatc ggtgttgaga ttgtcggtgt ccttgtagtt ccacacgttc
     5221 ccaagtcttc tgttatcgga tggatcgacc gaaacggttg ccgattgcgg cgactggtaa
     5281 gggaatttgt ctccggcctg aatatttctt ggatcagtaa tttttccgtg aattgccgac
     5341 catgctactg ttgctggtga agccagaaaa atctccgatt tttcgttgcc catgcgaccc
     5401 aaaaaattac gattggccgt gctgataact ttgaatccat caccgggaat tccctgccct
     5461 gtcccaagac aaggtccgca agatgaggat aacacatttg caccagccaa catcagtttt
     5521 tcgacataac cggctttcat agcttcgaga taaatagctt ttgaagcggg aattacaagt
     5581 agctgaaatc ctgcagccac tgttttacca tccagaattt ctgcagcagt cctgatatca
     5641 tcgaggcgtc cgtttgtaca tgtaccaata agtccctgat tcaattgagt gccttccact
     5701 tcagatactg ctttcacatt atcgacattg tgcggagcag caaccagcgg gaaaatcttc
     5761 gataggtcta tttcaatgct cttgctatat ttggcatcgg catcggccca cacaccgttt
     5821 agttttttac caaggtgttt ttcgagtact tcgtcagctg ggaacactgc atttttagca
     5881 cccatctccg aagcaaggtt agccagcgtc attctttcag agatactcag ggttttcaca
     5941 ccttcgccat ggtattcaat gctcatgtag ttggccccat cagagcctat catccctata
     6001 atccaaagcg aaatatcttt tgcgtacaca cctttctgca attttccttt gagggttatc
     6061 tttattgatt ccggaacaag aaaccaggtt tcccctttca aatacaaccc ggccgattca
     6121 gtacgatcga taccggcagc gagtgcgttg aaagcaccgg cagtgcaggt atggctgtcg
     6181 gaacccacta ttatcatacc aggttctgca taattggcaa ccaattggtg acaaatgcca
     6241 tctccggcat catggaattt tttaattccc tgttccttta caaaattgcg tatcacctga
     6301 taagaagtgg ccagtttggc acttgtggga ggtgcgttgt ggtcgagtac cactaacagc
     6361 tgatttgggt catgaacttt agtaccaccc attttgttaa aggtcgattt aatactctcg
     6421 gtattatcgt gggtaagaac aatatgaggc ttacggaaca caatcgctcc ggtatttgcc
     6481 tccagaactt tttctacaaa ggtttttcca gccatagttt tataatttgg ttatgttttt
     6541 aaaatcgcaa tgcgctaaga taaatcatag aaagcattaa aagtattgtt atgcagcatg
     6601 atttaaggaa tatttacacc tgaatgcatc ccttttttcc attttaacgc agtgtgacat
     6661 taattatgaa cttaggtgtc ttcatttcgt cgggcatatt gcgaaaatct atatttttta
     6721 tagtgtatgc ttcgattggc acattgttat caacgaaata tttgcgaatt agattttcgc
     6781 gcaatgtgag cagttggttt agcatcaatt gtgctttatc gtttcctatc ttctgcatac
     6841 atgcaccttt tactgagttt tcatcagttt ctggagtaag ccccatgtat gataaaaacc
     6901 cggtatcatt ccacggcatc gaagcagcat tcttgcggat aataccgaaa tcttcagaag
     6961 gtttctggtt cctcaggtat tgagttttac attcccttac ggcaagtaat tgtttctcaa
     7021 gatcaggatc ggttgtttgg ataaactcaa atgccagctt gtctttcttt ttcatcatcg
     7081 tagcaatctt actcaaattc gccgactgct cttcgctgag gctgtcctga agcattgaga
     7141 actcaatttg cttaaatttt tcggggtttc cgccaaaagc attcccaatt gcattgaaag
     7201 gtgctgcagc ggttttaatc ataaactcct ccaatgtttt ccaaattatt tttctcagtt
     7261 taaacgatgg ctccgagggg ttcccggtta cagggaggtc gaattcaata acatcgttgc
     7321 ggtcttttaa aatgtataat gcaagcccta tgggtacttt ataagcagtt gtatcctttg
     7381 tctttttacc catgtcaagg tcaacaattt taatatgatt gttgttttcg agtgcaacag
     7441 gagtcatata taccgagcaa tcgtaattca ttactccttt tgtaatgggg cgtgcaagga
     7501 aatattcagc atagggcgac aaaaagacca tatccatgtt cttaacactc aacaccatat
     7561 cgagaccatc aagcgttagc atattcactg ttccgttccc gctaatcttc ccggtttcgt
     7621 ttattaacat tttaaaatcg aggggaacct tggtcgccaa atcggtataa ttgtccatac
     7681 ggaattcaat atccgaaata tccattataa aaggtctgtt cagggtgtaa tcgtggtacg
     7741 aaactgtacc gttagtaata cttagcttat cgatgttgta atgcatctca atggtatcat
     7801 ccggatcggg caaggtatca gcatagaaag gtgcaagcac cttgtcgtag tttgccccat
     7861 cttttgccag aacggcagaa attataggcg agttgatttc tattgaatta atgcaatagt
     7921 tttcgcgcaa caaattcagt gagtcgataa taacatgaat atcctgcacc gtggcaaaag
     7981 gtttcatgtc gggaccggtt aaaagtaggt tttgaacccc tgcttcaccc ttcactatta
     8041 tattcattag gttgtcgatg ctgccggtaa tgcttaggtc ggtataaagt gcgcctccca
     8101 tagagccaac atccacgata tcggttaggt attccttcag caacgaggcg tcaagttgct
     8161 cggttttggc atgcaattca tacagccctt tttcctgatc gaatgcaccg ctaagcgaaa
     8221 cttttccgtt ttcgcccatc tcaaaatcga ttcccaattc cgaccggctg ttatcccatg
     8281 caatctcggg aatagttatt cctaggttct taagctcggt tacactggca atggttttat
     8341 cttcgtaacg tataaatccc tccgtgatat tcaggttttt tacaaggtac tttactactt
     8401 catcagagct gtcaggctca tcggaagtat caaaaaaagc aatgatatcg tcgaaattaa
     8461 aacctttttc atcgtatatc acactgatat taggtttaac aagctttatc tctgagaaag
     8521 catagcgatt gctgagtagt tgccatgggt cgaaatcgat gcgtaattca tcaaaactta
     8581 caaaactgtc tttttcattt tgttcataaa gtgtaaaccc ttttacagaa acactgaatt
     8641 tgaggtaatt gatatgcaat tcgctgatgc tgatgtttcg gttcaccaaa cttttgccat
     8701 tgttttcgat ataccacttt gcaatgtcgg agaggaaaaa caacaatacc acaagaagaa
     8761 ggacgagaac aataaaaatt tttaatccgg ttctgcgctt gcgcttctta ggtggttttt
     8821 gcggtaaact ctcttgtggt actacagatt gtcgttcctg atccatttca gtcttagggt
     8881 tatcatgtgt ttcatttacc ataatagcaa aatgtttaat aagttttaat tgaagtaaaa
     8941 atgatatttt tcagattata aatataatat tttccgtgtg acagaaaaca gggaaaatta
     9001 ctcaggtttt aagttttttc ttttttgcag ccgggaaaag tacattattt aaaataaggc
     9061 ggtatcccgg tgagtttggg tataacgaca ggtcggtttt ctggtcaccc acataatgcc
     9121 gatagtcttc aggatcgtga ccgccataat atgtccacat tcctttgccg tattcaccat
     9181 gcaggtattt aacctcgccg acagacttgt tttcacccat cacaagcaca tcgggtttta
     9241 cgaagcgact gtcgaaggca gtagtttgcc ccatgaacga atgtatcata ttgttatgat
     9301 tctggcaaag catggttgga acagggtccc attttgccga aaactcaaaa agagtgaaat
     9361 aatcttgttc ctgactcatt tttcgggtat ttgagacatc gatattggaa tgctcatatt
     9421 ccagcgggtt ttgaattagg gtatagtcgc ggaaagcaaa cccttttgag aagtcgagct
     9481 tctgctgagc cacaggatct gcggcatcgc catcaaacat ctgggcgcaa atgtcgacac
     9541 catcggaagc gagagcaatg tcgtaggtgt cggtagcgga gcacatggca aacatgaatc
     9601 cgccattggc aacataggct tttatggttt ttgcaacaaa tagcttgagt tgcgatactt
     9661 tgttgaatcc cagccgggct gccatggctt ccatttcgtt ctgctggttt tggtaccatt
     9721 gcatgtgccg gtaagaaccg tagaacttgc catattgacc ggtaaaatct tcgtgatgaa
     9781 gatgcaacca gtcgtacttg ctgagctctc cgttaacaat ttcttcgtca taaacaatat
     9841 cgtacggaat ttcagcatag gttagggcaa gagttacagc atcgtcccag ggcaatttgt
     9901 tgggtgggga atacacggca attttaggtg ccttttcaag cttcaccgcg tccatgttta
     9961 catcaggctg agatatctca ttgaaaatgg cgtttttttg agcatcggca atgatttcat
    10021 agctaacacc cctgatgttg cattcgcttt caatttcttt ggcaaaaggc attagaaaac
    10081 tgccaccgcg ataattcaac aaccaccatg cttcaatctg attttgaagt atccaatacg
    10141 tgatgccata ggccttaaga tggtttttct gactttcatc catcggtagc agtatgaatg
    10201 caggcttagc aactatgctg cttaaacaaa gcagtagaaa taatatcgaa acgcgaaata
    10261 gcttcattgt aaaaaacaaa tttatctaaa ttttacagca atatacaaat aacatgcgca
    10321 aatgattact tcaataatta aaacaatgct tataattatt ttattgcagc gcaacaaaaa
    10381 aatcattttc ccttgcgcca tagcatgcct gtacgcgacc tgctcccagc tgccgaattt
    10441 gaatagccgc tgctcacata cctgatatct tcgatagtgt aaaatgcatt gggattgaaa
    10501 ttctgaatca gcggtatgag ctctttcagg ctcgaccttt ttactgtaag gaatattaca
    10561 ttcacttctc cttctctgcc ctgtgcattc ataaaggtta gtccgtaccc cttttctttt
    10621 agtgtttgta taagctgcat accactgcgt gcagtaacta ttcttactcc aacagtaccc
    10681 atggcaagct tttcttcaag tatcatccca atgtaattgc ccgcagcaaa acccgcagca
    10741 taaaatatga aatttaccca gttgtcgaga tgctccataa ttttacttac agcgataatc
    10801 catatcaata cctcaaaaaa gcccagtagc ggtgctatga gtttttttcc tcgcgatacc
    10861 ataataatcc ttatagtacc aatcgataca tcaaaaatcc ttgctgtaaa tatcagcaag
    10921 ggcaatatca gataattaaa ccattctgag ctcatagatt tttcattttg cgcaaaggta
    10981 aaaagctttt ttaatggaaa ttctaaaaca gttaataaaa tgttttaaga attagatttt
    11041 cagtttatta caatcttatt ttttttgtaa atttgtatct atattgtatt catctgtccg
    11101 atttttaatg tgtaaaaaca atacgattat gaaattacat ggtttgattg ctgcagtttt
    11161 gcttttaact gcgaatgcat ttgcccagca gggcttttat gcagggtttt cacagcctca
    11221 cgaaaatgtt tacgaaatta gtttcgatct tgccgattgg tcacttaatc aggtgactca
    11281 cgatggtgtt atttatcaga ccattgctat gggaacatct gcggtttgtg acaaaaaggg
    11341 ttgggcagaa ttgccggtta taagtgctgc cattcagctg ccaccagaaa aagacgtgga
    11401 tatagcggtt gcagaagctt cgtattacga cattcaactt cagcacccga tgcttccatc
    11461 gaggggtata attgcacgta gtgaagaccc atccacaata ccttactgga cagatccagc
    11521 ttcgttggtt gatgaattct acccccaaca gcatattggt atggatgaac cctttatttt
    11581 ccgcgatgtc agaggtactg cggttcgagt aataccgttc cgatataatg cggcaacagg
    11641 tgttctcagg gtatacaccc gcattgtagt gcagcttacc gaaaataacc acactccaac
    11701 aaatccgctt attgtggata atacaaaccg aattttagaa gtagaaggta tctacaggag
    11761 tatgtttctt aactattccc cttcagcaac aaaaacagac cttgctatgg acgaatacgg
    11821 tgatttactg gtgcttgtac ctacgtcgta tgcttctacg attacaccct atgtaacctg
    11881 gaaaagagaa atgggctata atgttacggt tactaccgtt tcaacaggaa ccaatgttac
    11941 atcgacaata cagacagctt acaacaataa cccgaacctg ctgtatgtgc agcttgttgg
    12001 cgactgggct gatattaaat cgaacacgct aaccagttca gtagtgtcat cgtcttacaa
    12061 tgttggagcc cccatggatc cgcagatggg ttgcgtttcg ggtaccgaca attttgctga
    12121 tattgccata ggcaggttct catgcgctaa tgcagcggac ctcaccgttc aaataaacaa
    12181 agccattaaa tacgaaaaag aaccagacaa tgtggcggga tggcgagaaa catttatcgg
    12241 tgttggctcc gaagaaggaa ccggtgatga ctcagaatat gactatcagc atattcagcg
    12301 tatctatacc cagcgtctcg acggatttac ctatgacacc caccgccaga attatcaatc
    12361 aggtgcaaat gccacaactc tcgcggggca tatcaacacc ggtggctcaa ctattgcata
    12421 ttgcggacat ggcgattata cacaatgggt tacggcaagt ttctataaca caaacgtaaa
    12481 taatctttct aatggtgata agttgccttt tgtggtttct gtggcctgtt tggtaggcgc
    12541 ttttcataac agttctgtag tgtttgccga agcatggcta cgcaaaagcg gtggcggagc
    12601 tgtggttggg tggtttgcaa ctataagtca gccatggaac cctcctatga ggggacagga
    12661 ttatttctat gatatactta tcggagggtt caactactca tcctattccg gacagagcgg
    12721 ccttaccact tcggagcagc gcactcattt cggatccatt gtggtaaacg caatgaacct
    12781 catgcttact gagtcgaaca catcgcagga taccgaaact acaaaaacct gggcaacctt
    12841 tggcgatgcc agcctgcagc ttcgcacaaa acaacctgtg gcacttgttc ttagcaacac
    12901 cagtcctgct gttggtactc cttttgccgg aacggcatat attggtagca ctcccgctgc
    12961 caatgtgctt atttgcattt cgcagaacgg atcctactat catggactaa ccaactcatc
    13021 gggagcatat tctattaccc atagtctgac tcagggagat gcactgttag tagcaaccgc
    13081 ctttaataca actactatct accaaaccgt tacctgtgtg ggaggcaccc catgctgggc
    13141 ggtaaatacc cttagtggaa cctctgacgg atcaactgtg aacctgacgt ggtcagctcc
    13201 ctccgaaggt agtgtttccg ggtataatat ctaccgcgac ggcgtattgc agtcaactac
    13261 cacaagcact acctacacac agagttccgt tcctaacggg acatacgaat attgtgtggc
    13321 tgcattgttc aataacgaag aatgctacct acgcgaatgt gttaccgtgg ttgtaaacga
    13381 cggcagtaac aatgactgcg attctccttc tgacctcggc attgcggaag taaacagcac
    13441 tacgcataca cttacatgga tagaacccga aggtgcgggc aatattgcag aagatgttga
    13501 gtcgcacacc tcatggacac ttaactctcc tggctctgtg gggtggtcat atatcgacgg
    13561 tgatgcctta gcaacatttg gtgccagtgc atttaatttt accaatgaaa gttcggctat
    13621 ggcctatatt gttttcgacc atgctggcac tactggaggt acccccaatg ggagtgcaca
    13681 ttctggtaac aaagtttttg cagatattag tccgtccaat tcatctgccg ctaacgactg
    13741 gattatcagc ccggaattaa actttgatga agcatttact ttctcatttt atgctaagtc
    13801 atatgtaagc acatatggct tagaacgctt caaggttgct tattctacaa caggaacaca
    13861 acaggcaaat ttcacatttg taacaacaaa ctatattgag gcaccagtta catggacact
    13921 atatacatac acaattcctg ctacagcaaa atatgttgcc attcaatgcg tttcagcaga
    13981 tgcatttata tttatgcttg acgatttgta tattggcgac gggggaggag tggcagaagc
    14041 tcccataggc tacaatgtgt attgcgataa tgtatttctt ggaaacgctg caggtacaaa
    14101 ttttacaaat tcttcagcca caacaggata tcatcagtat tgtgttgaag ctgtttatgc
    14161 cgatggttgt atttcgccgg agatttgtgc tactattgga agtacggcaa cctataccat
    14221 agccgcaact gccggggcta acggtactat aagtcctgca ggaactacaa cggtaagtca
    14281 gggtggcagt cagacctata cgattactcc caatgcttgt tatcatattg ccgatgtgct
    14341 tgttgacgga gtcagtgtgg gggcggttac atcatacact tttacaaata taaatgccaa
    14401 ccataccatt gcagccacat tcagtgccac tacctatgcc attactgcat cagcaggaag
    14461 cggtggtagc atttcacctt caggtggtac tgcggtgcct tgcaacggaa gtcagacata
    14521 cactatttct cccaatgcct gctatcagat tgccaatgtg cttgttgacg gtaccagtgt
    14581 tggagctgtt acaacataca catttaacaa tgtaaatgcg aatcatacta tttcggtaag
    14641 tttcagcgtg ataacataca gtataaacgc atctgcaggc acaggaggca gtattactcc
    14701 ttcgggtaac acaagcgtaa cctgtaatgg tagtcagaca ttcacaataa ccccctcctc
    14761 gtgctaccaa attgatgatg tactcgttga tggaacaagt attggcgcag ttacaaacta
    14821 cactttcaat aatattaatg cgaaccatac tatttcagtc agtttcgaat tactgactta
    14881 taccataact gcttctgctg gtgcgggagg aagtatttcc ccatcgggaa gtgtagatgt
    14941 aaactgcgga tcgggacaaa cattcgaaat tgctccggag aatggctacg aagtggcttc
    15001 agtgttggtt gatggtaatg acgttggagc tgtttcatcg tacacctatt caaacctgac
    15061 atcaaattcg accattgtgg ccaattttag cctgataaca gtggaagaat gcagcgcacc
    15121 ggaaaatctt agtgccactg ctacagctga ttatattgtg ttgaactggg atgccgcacc
    15181 caatgcaata agctacaatg tgtaccgtaa cggtgctctg cttgtaaata ctacatccgt
    15241 tcagttcttc gatgtcactg ctgaagaaga ggttgaatac tcctactatg tagtttcggt
    15301 gtgcagtgac tccgaatcag atccgtcgaa tccggccagt gctactatca atgtaggcat
    15361 aattgaaagc aatgataaca caattactgc ctatccgaac ccaacttcgg gaattgttga
    15421 aatagtcctg aaaaatgact ctgacaatta tcatggtttg gttcttacat ctctcgatgg
    15481 taaaatgctt ctaaatagtg tgattgaagg agaaacgatt agaatagatt tgtcggactt
    15541 tgccgatggt atgtatatta tcagaatttc agggagctct ggcaatgata aaatcctaca
    15601 tattgtgaag caataaaatc tgataattaa aaaaatggag atttcctcac ggcagtctcc
    15661 attttttttg cagataataa caggtcttct aattctgccg tttaaaaata taccttgtaa
    15721 tgaaaatgcc gttatcgtcg tccttgctgc catgactttc aacgccggta acaacaaagg
    15781 cgagctccca gccatcggca atcatctgat tgattttggc tgagattaca gcgtcgttcg
    15841 atgcaatgtt cctaaaattt atacctacag cactgtagaa attgagcaac tttgtctcct
    15901 cgaatttgtc gattttaatt tcatcgcgac taacctcact ctgctttgtg tctgtgcctt
    15961 gttcacgata ggtggtaaac gctcctgcat caattttttc ctgatgctca atgattcgag
    16021 aacgacctac acccatagga acaatcgatt ctacgcttgt gattactttg tattcctgcg
    16081 cctgaattac agcatttaga agcagagtag ctgaaacagt taaaattaat tttttcatag
    16141 acttggtttt tatgaggttt tatttccagc gttttaaatc acctgaaaca atagaactgg
    16201 atcgaggcat gcaatgaaat caggtgtggt gagcgactcg gtgttataga ggacctcact
    16261 ggttgtgcta atattgatta catgtccgcc cgaggctcta acgagcgcat gcccggctgc
    16321 aatatcccat tcgttgatag atcccattct tggatataac gaagcggttc cttcggcaat
    16381 catgcagagt ttcagactgc tcccgatgct cacatttttt gtgtcttgaa atgtattcct
    16441 gattttttcg gtaaacattt ctgtggcgat atttttgtgt gatttactcg atacaatagt
    16501 aaccaactca ggcttttccg atggaagttt aactattgaa gtgagttcat tattgctatg
    16561 aaccaatgcc ctgaatgcat tggcggttgc atatccaaaa tagattgtat ttagggcagg
    16621 ggcaaagatt actccaagaa ccggtacccg attatgaatc aacgctatat ttacggtgaa
    16681 gtcattgccg cccttgatga attctttggt tccgtcaatg gggtcaatca accagaatgt
    16741 ttcccaccta attctttcgg tataagaaat ttgcgaagat tcttcactaa tgatgggtat
    16801 ccttgttttc ttcagaagct gtgcgataat ggtattggct gccatgtcgg cttctgatag
    16861 cggggaaagg tcctgcttgt attcaacgtg ataaacgctg ccgaaaaatt tcatacaagt
    16921 ttttcctgct tctatagcag caggtatggc aaagtcatgc atcaaactta ttagtgcatc
    16981 attaccaatt accattcttc ttcgataatc ttattgatat tgtaagcatt ggcctgagca
    17041 cttggaacaa taagcatatc gttgatgttg acatgcacag gacgggttat tgcataatga
    17101 atagcatcgg caatatcttc ggcatgaaga ggttcaaaac ctttatatac ctcttttgcc
    17161 ctttgtttat caccatgaaa acgaacctcg gaaaattcag tttctgcaag acccggagca
    17221 atctgagtaa ctttaatatt gtgcttgagc aaatcgatgc gcattgcttt cgacagggca
    17281 tccacagcat gttttgtggc gttatataca ttgccgtttg gatatacttc tcgacctgca
    17341 atggatccaa tattcacaat gtggccgctt ccctgagcta taataagggg tataactttt
    17401 ttgcttatat agagtaagcc ttttacgttg gtatcaatca ttctttccca gtcgctgatc
    17461 tcgcaatcct gaaatgggct gaaacctgct gcaagtccgg cattatttac cagaatacta
    17521 atatctttcc attccgttgg gagactatcg atagcattat ccacttcttt gcggttgcgt
    17581 atatcaaaac acaggctgat gatactgcaa ttatgcttca gtttgagggt atcctgcagc
    17641 tcactgagtt tgctttgccg gcgtccggtg ataataatcc tgtagccatt ctttcctagg
    17701 agttctgctg ttgccttccc tattcctgca gtagcccctg ttattaatac aatcttattc
    17761 attgcgtgaa aaatttcgtt aaaattataa aaattactgc attatcgtcc ccaaattttg
    17821 aaattttaaa attccaaatc actgttgtcc gattaaaact ttttaaagaa gccagcgcat
    17881 aagtctggct tct
//
LOCUS       DAOOQP010000057        17786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_3862_length_17786_cov_5.491230, whole genome shotgun sequence.
ACCESSION   DAOOQP010000057 DAOOQP010000000
VERSION     DAOOQP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17786
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_17786_cov_5.491230"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLK75_07345"
     CDS             <1..219
                     /locus_tag="PLK75_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39100.1"
                     /translation="CTSLRILVPETSASTNSATWAFQSGCKNNNFNDNQTVFFSEVKL
                     IWHDIALFFVELLGKLKKTTNFATRLKN"
     gene            252..602
                     /gene="rpsF"
                     /locus_tag="PLK75_07350"
     CDS             252..602
                     /gene="rpsF"
                     /locus_tag="PLK75_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOY39101.1"
                     /translation="MNQYETVFIATPVLSEAQMKETVDKFRSIIQANEGEVVHEEDWG
                     LKKLAYPIQKKSTGFYHLFEFKADSAFIGKLETEYRRDERIIRFLSFKMDKNSIEYSE
                     KKRSKKQEKETKEE"
     gene            605..874
                     /gene="rpsR"
                     /locus_tag="PLK75_07355"
     CDS             605..874
                     /gene="rpsR"
                     /locus_tag="PLK75_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOY39102.1"
                     /translation="MAQSQSEIRYLTPPSVEVKKKKYCRFKKSRIKFIDYKDPQFLKK
                     FLNEQGKILPRRITGTSLKFQRKVSQAVKRARHLALLPYVTDMLK"
     gene            892..1341
                     /gene="rplI"
                     /locus_tag="PLK75_07360"
     CDS             892..1341
                     /gene="rplI"
                     /locus_tag="PLK75_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOY39103.1"
                     /translation="MEIILKQDVANLGHKDDIVKVKDGYANNYLIPQGFATLATPSAK
                     KMHSENMKQRAHKEEKLRAGAQTFADKLTGSRFKIATKVSSTGKIYGSVNTIMLSEAL
                     LGSGFEVDRKNIKIKEEEVKEVGVYHASVKLYKDIRVEFEFEVVAED"
     gene            complement(1419..1793)
                     /locus_tag="PLK75_07365"
     CDS             complement(1419..1793)
                     /locus_tag="PLK75_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOY39104.1"
                     /translation="MKRIITTNKAPKAIGPYSQAVEANSMLFISGQVPVNPENGQIPD
                     GITAQTEQVMKNIGAILDEAGYTFSNVIKSTCLLSDMENFKAMNEVYGRYYPENPPAR
                     AAFAVKGLPLGALVEIETIAVK"
     gene            complement(1799..2875)
                     /gene="buk"
                     /locus_tag="PLK75_07370"
     CDS             complement(1799..2875)
                     /gene="buk"
                     /locus_tag="PLK75_07370"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015240178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOY39105.1"
                     /translation="MKQYRILAINPGSTSTKVAVYENEEKVLVKNIKHSTEELAHFKN
                     IASQYEFRKEAVLKELITGGLDINSIDIIIGRGGLVHPVTSGIFEVNEAMINDLFNPP
                     MGEHASNLGGLIAHDIAKSLNNVRAYITDPVVVDEMKDIARVAGHPLFKRVSIFHALN
                     QKAIARRYAKDSGKVYEELNLIVVHLGGGISVGAHEKGNVIDVNNALNGEGPFSPERS
                     GTLPAWQLAQLCFSGKYTPAEVKKMITGQGGFVAYVGTNDAYEIEKRCQAGDTQAQFY
                     SDAMAYQVAKEIGSAGAVLKGKVDCILLTGGIAYNKQFCSFIEDMVSFIAPVKVYAGE
                     DEMYALAMNGLMVLRGEMQTKIYK"
     gene            complement(2891..3793)
                     /locus_tag="PLK75_07375"
     CDS             complement(2891..3793)
                     /locus_tag="PLK75_07375"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOY39106.1"
                     /translation="MALKKLNELIGIAKAKGVRTIAVAASADKHVLEAVHHATQQGFV
                     KPLLVGNAEITRNILNEIGFKLADDQIIDEPNDVVACKKAVNLVREGKAELLMKGMVT
                     TAILLKAVLDKENGLRVGDTLSHLAIFQSDFYHKLIAVTDAAMNVAPEFNEKVAIINN
                     SVEALRGIGIDTPKVAILGPVEVVNLKIESTIHAAMLTQMNRRGQITGCIIDGPLALD
                     NAVSKEACEQKKIITDVGGDADLLVAPELNAGNMLYKSFAFMGGALLAAVILGARCPI
                     VLTSRADSEETKMLSIALAAAMLK"
     gene            complement(3954..5168)
                     /locus_tag="PLK75_07380"
     CDS             complement(3954..5168)
                     /locus_tag="PLK75_07380"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOY39107.1"
                     /translation="MKVLVLNCGSSSIKYQLLDMQAEAELLAKGLVERIGLPEGVLTH
                     KGPHCKSLELNQPIPDHTVGIKIILDILVAPETGVINNLNEIDAVGHRVAHGGEYFDQ
                     SAFITTEARKNIENCCSLAPLHNPANLKGIDAMITLLPGIPQVAVFDTSFHQTMPEYA
                     FFYPLPYEYYEKYKIRRYGFHGTSHKFVAEKACKALNWDIKERKIITCHLGNGASICA
                     IDKGISVDTSMGFTPVEGLMMGTRVGDLDLGAILHIMKADNIDVESANNLVNKKSGLQ
                     GFSGVSSDMRDLMEAGKKGNKRADVAIEMFAYRVRKYIGSYVAAMNGIDLIIFTGGIG
                     ENAWPVRERIASHLGYLGAHFDSNLNENTRGIDGTISTPDSKVKIMVVTTNEELVIAR
                     DTLRIVSAMKVK"
     gene            complement(5208..6212)
                     /gene="pta"
                     /locus_tag="PLK75_07385"
     CDS             complement(5208..6212)
                     /gene="pta"
                     /locus_tag="PLK75_07385"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOY39108.1"
                     /translation="MELLNQIKESARNKTMRIVLPEGTEERTLKAADQAISEKLASII
                     LIGNKAEIESMADKFGLKNIKNAYIVDPKKHDKKDTYIQTLYELRKNKGLSLEEAANL
                     VENPLYLGVLMIKCGDADGEVAGADNATGDVLRPAFQIVKTLPGISVVSGAFIMILNN
                     PEYGENGIMIFADCAVHPDPTAAELAQIAVATGLTTRAIAGFEPRIAMLSFSTKGSAS
                     HAMVDKVVEATRLAKEMAPDLQIEGELQADAAIIPSIGQKKAPGSKIAGHANVLVFPS
                     LETGNIAYKLVQRLANAEAIGPVLQGMAAPINDLSRGCSVSDIVNLIAITANQAAAKK
                     "
     gene            complement(6368..6910)
                     /locus_tag="PLK75_07390"
     CDS             complement(6368..6910)
                     /locus_tag="PLK75_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOY39109.1"
                     /translation="MRIVAGILKGRHIEPPKSFDARPTTDFAKEGLFNVLNNYFDFES
                     VRALDLFAGSGNISFELFSRGCSDITAIEINDKYVKSIISYAARFDCNSIQVLKTDVY
                     GFLKHCRQTYDIIFADPPYQEANYQLINELVFSNQLLRENGWLIMEHSRDTDLSKLQN
                     FKQLRKYAGVNFSVFYIENY"
     gene            complement(6901..7746)
                     /locus_tag="PLK75_07395"
     CDS             complement(6901..7746)
                     /locus_tag="PLK75_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOY39110.1"
                     /translation="MGAFAIEDIELKNTETNTLDLSIQISANGLSYSYYQRNTNKYLG
                     IYHIPEINDTAKLITFFEEKSDLRNNEFAKVRIVYCTPECTIIPAVLFNENDTDKIFN
                     LSFGDATKRKPLAIYLPASRNYMVYGIPEKLNNYLNFAFPAKLTYAQSAPFVDYGFTR
                     HLLNNDRPLHTIFTDVYGDYFSVMLLENDKLLLFNTFRYKSANDIVYYLLSIFNQYKL
                     DQAKTHCSVSGFIEKDSIAIIQLRKFVQFVFFEGRNTHFNYCYRFQEVPAHMFYNFLN
                     IVECE"
     gene            complement(7764..8375)
                     /locus_tag="PLK75_07400"
     CDS             complement(7764..8375)
                     /locus_tag="PLK75_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39111.1"
                     /translation="MKILNISIKLVLVAAAVVLVYFIVAGIRKPIDFEKQRADRYQKT
                     ISRLIDIRTAQVAYKSIYGCYTPSFDTLINFIKNGKMPVVKAIGSVPDSLTELQALKM
                     GIIKRDTVYLNIKDTLFKHIDYPIEEICRIPVGTKVKFRMDTASVMTGSEVLVKVFEA
                     KANNWDILQGLDRQYVINFNEEKDSVLKVGSLETANNNAGNWE"
     gene            8458..9879
                     /locus_tag="PLK75_07405"
     CDS             8458..9879
                     /locus_tag="PLK75_07405"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY39112.1"
                     /translation="MIKNFFTRTVITNLQHEPTQSQLKALDSIFDFLSNGNEKMVFIL
                     RGYAGTGKTSLLSALIHTIPVFKMNSVLMAPTGRAAKVLTQYSGKMAYTIHKCIYRKK
                     NKTETGGFELNFNRNSDTLFIVDEASMISDTRQNGNVFGSGYLLQDLIDFVFSGKNCR
                     LLLCGDDAQLPPVEISESPALDTTYVSDLGCTCMSAQMNDVVRQVFDSGILFNATVIR
                     KLVQHQKTLIPVLSTANYTDIVIIDNACLTETIEMCYSQYGLGQTKIITRSNKQALRY
                     NQGIRSRIFSYESELVCGDQLMVVKNNYFWLPDDYPVDFIANGDMLQIKKIRKSQELY
                     DCRFIDATVTLSDINDIELDVKLLADAISVGNASMPHDFYTKLYADVEADYAHIDKKK
                     NRVEAVMNDPYFNALQVKYAYAVTCHKAQGGQWQAVFIDLGYFETLEPDIALLRWLYT
                     AFTRATEKLFLVNFKAEFFNDNP"
     gene            9923..10834
                     /locus_tag="PLK75_07410"
     CDS             9923..10834
                     /locus_tag="PLK75_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39113.1"
                     /translation="MISICIPIYNFDVRPLVGQLFKEIQSLNNGCEIICIDDDSDDYF
                     KNINGNFCDKCGEYVQLYENIGRAKIRNLFLKYAANDYLLFLDCDSIVTNDNFVKNYL
                     LCLSENPSVVCGGRVYPPERPETHFLLRWKYGHKRESKPASLRKRKPHKSFMTNNFII
                     HKSILLSIQFEERISKYGHEDTLYGFMLQQNGIPVVQTDNSILNGELETNAEFLAKSE
                     MALENLAFIIAQLNENREFISNVTILRWYYRLKAIKLLFVISLMRVFFLRCFHKRLIS
                     GKACIRIFDMYKLILFDKFYKKFFSSR"
     gene            complement(10888..11505)
                     /locus_tag="PLK75_07415"
     CDS             complement(10888..11505)
                     /locus_tag="PLK75_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOY39114.1"
                     /translation="MKIICIGRNYSEHVTEMKSEAPENPVFFLKADSCLLTRNRPFYI
                     PEFSNEIHYECELVLRISKVGKYIEERFAHRYYDAVTLGIDFTARDLQQECKKAGLPW
                     AISKSFDGSACLGSFFSLTDIPDISSIPFRLYRNDMLVQSGNSAEMIFSFDQIIAFIS
                     RYMTLKTGDLIYTGTPAGVGEIEIGDSLTAYLANLPKPVIQMKIK"
     gene            complement(11555..12325)
                     /locus_tag="PLK75_07420"
     CDS             complement(11555..12325)
                     /locus_tag="PLK75_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOY39115.1"
                     /translation="MELNLKKPLIFFDIETTGLNIINDRIIEISMIKVLPNGREETKS
                     YRFNPEIPIPEETTAIHGIRDVDVATCPTFKSTAKEIATFIEGCDLAGYNSLKFDIPM
                     LVEEFLRADIEIDLKKHNFVDVQVLFMKKEPRTLSAAYKFYCNKEHSEAHSALGDTLA
                     TYEVLKSQLDKYNDLPNDVVKLAEISSYGELADFAGRLIFDEKGREMVNFGKYKGQLL
                     EEVFRKDPGYYSWVMQGDFPLYTKKVFTRVYVRFKSLK"
     gene            complement(12381..13511)
                     /gene="dnaN"
                     /locus_tag="PLK75_07425"
     CDS             complement(12381..13511)
                     /gene="dnaN"
                     /locus_tag="PLK75_07425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOY39116.1"
                     /translation="MNFVISSTSLLSRLQIVSKVVSSKNTLQILDNFLIRVESGIMHI
                     TASDLESTLTAHLQLDSASGDGSLGIDAKRLLDVLKEFPEQPLTFNVDDESLKIDINS
                     DNGKFSLIGVNADDFPQLPVMNSEKSTNISLKPEMLYSGITSTLFATANDELRPVMNG
                     ILFEMSPDGVNFVATDAHKLVRYKRTDVVAEVNDKFILPQKPAAILKNILPKASGEVT
                     LEFDNKNAFFTFKDFQLVCRLVEGTYPNYSAVIPSNNPNKLTVDRVEFYNTLKRVSIF
                     SNQASNLVKLHITGNQLTISAQDIDYSISGYEMINCLYEGDEMDIGFKSGFLMDILQN
                     LSTSEVVIELSDPTRAGLVLPKDNENTSEHILMLLMPMMINN"
     gene            complement(13622..14689)
                     /locus_tag="PLK75_07430"
     CDS             complement(13622..14689)
                     /locus_tag="PLK75_07430"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOY39117.1"
                     /translation="MKRRNLILLIILIVLLVAAIIVFFTRYHIVSYESYGETHYRIER
                     IKGTLKVLRDFAVNDTASINKIFLADKQNNTILLERKNDHWTVNGDNIARRDFVDVLL
                     ETIKDVEVAAPVPKSKLDYVLRSISTNSTKVEIYQDNTLVKTYYVGGVTPDNKGTYMI
                     LEGSSAPFEMHIPGFTGFLSTRYITDLFEWKDRILFKYSASDIKSVSLVFPADPENSF
                     MAVNNGNNSYSLISLETGKPVTEADPMMIKEFISRYKFVGFESFLNGTTEYQKLDSIL
                     KSPEMCIYTVEDRSGTKKTLKTFSRPNFKNVYDDEGNFYNNDVDRFYGVINNDKDLVL
                     LQYINFDLLTKKLSDFRGANP"
     gene            complement(14686..16443)
                     /gene="gldG"
                     /locus_tag="PLK75_07435"
     CDS             complement(14686..16443)
                     /gene="gldG"
                     /locus_tag="PLK75_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOY39118.1"
                     /translation="MIGKQKQPAGITRRTLKRQGLIQLTLVLVIVALINFIGNLWFYR
                     LDLTAEKRYSLSEVSKKVLHNLDDVVYIKVYLDGENLPLGFKRLKRSVQEKLDDFRVY
                     SDNIEYEFIDPFKDADETKKRDIFIQLIKKGLMPETLQIMGDAEYTEQVVFPGAIIVY
                     NGQEYPVNFFIDDLSVGDQQQFDKTVSELERSFIHGIWLLSRPAKQKIAFIEGHGELD
                     EYQTYDIMMELSQYYQIDRVSINGILNSLNGYRAIVIAKPDSVFREKDKFIIDQYIMN
                     GGKVLWLVEWMSADMDSLASRPEDMALIRDINLDDQLFRYGVRINPDLIQDLKCLSIP
                     IMMNSSGGQPQFAPVPWYFFPVIIPDSLTNHPLTRNVSRIRTEFISSIDTVGDNTQVK
                     KTILLRTSQYSKSKMAPVQISLDLIKDRPDPATFNNPRIPIAVLMEGCFESNFNNRIP
                     PEIQNSNEINFAPISKPTKMIVISDGDMIRNEVKTLGNKKQPYYLGEDKYYQEQYCPG
                     NREFLVNCINYLCEDEEMIALRMRNIKMRELDRTKVKQDGVFWVWFNTAVPIAIIILT
                     GIFTVVIRKYKYGRNKTKK"
     gene            complement(16449..17180)
                     /gene="gldF"
                     /locus_tag="PLK75_07440"
     CDS             complement(16449..17180)
                     /gene="gldF"
                     /locus_tag="PLK75_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOY39119.1"
                     /translation="MGALLWKEISEFFNSIIGYIVVVVFLLINSMFIWIFHTDFNILD
                     VGYAGLDSLFILAPWIFLFLIPAVCMRLFAEEKRSGTSEWLLTKPISELEIVIAKYIA
                     ALILSAIALLPTLIYLISLLVLADPPGNLDLGGIAGSYFGLFLLAAVYASIGLFASSL
                     TRNQIISFFISVILCYFFYLGFSQLASLSTNGSIQDFIIALGINDHYTSMSRGVIDSR
                     DILYFYSVITLFVFFTKLSLESRKW"
     gene            17430..>17786
                     /gene="glyA"
                     /locus_tag="PLK75_07445"
     CDS             17430..>17786
                     /gene="glyA"
                     /locus_tag="PLK75_07445"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOY39120.1"
                     /translation="MKRDDLVFDIIRKEKERQMHGIELIASENFVSDQVLEAMGSVMT
                     NKYAEGYPGKRYYGGCQFVDQTEQLAIERICELYGAEYANVQPHSGAQANASVFLACL
                     NPGDTFMGLDLAHGGHL"
BASE COUNT         5139 a         3584 c         3550 g         5513 t
ORIGIN      
        1 tgcacatcct tacgaatact agtccctgaa actagcgcgt ctaccaattc cgccacctgg
       61 gcgtttcaat caggctgcaa aaataataat tttaatgata atcaaacggt tttttttagt
      121 gaagtaaaat taatttggca tgacatcgct ctatttttcg ttgaattatt aggtaaatta
      181 aaaaaaacga ctaattttgc gactcgttta aaaaactagt attaactaat aactaaagtg
      241 cgaaaattat gattaatcag tatgaaaccg ttttcattgc aactcccgtt ttgtctgagg
      301 ctcagatgaa ggaaacggtt gacaaatttc gcagcatcat tcaggcgaat gagggcgaag
      361 ttgtgcatga agaagattgg ggccttaaaa agcttgccta cccaatccag aaaaagtcca
      421 caggttttta tcacctgttt gaattcaaag ccgattcggc attcattgga aaactggaaa
      481 cagaataccg tcgcgacgag cgtatcattc gcttcctgag ttttaagatg gacaagaatt
      541 caatcgagta cagtgagaag aaaaggagta aaaaacagga aaaagaaact aaggaggaat
      601 aattatggca caaagtcaat ccgaaatcag atatctgaca ccgccatcgg ttgaggtaaa
      661 aaagaaaaaa tactgccgct tcaagaaaag ccgcataaaa tttatcgact ataaagaccc
      721 gcagttcctg aagaaattcc tgaacgagca gggaaaaatc ctgcctcgcc gcattaccgg
      781 tacctcgctg aaatttcagc gcaaagtgtc gcaggcggtt aaacgcgcac gccatctggc
      841 tttgctgcca tatgttacag atatgttgaa atagtaaaca aggaaacaat tatggaaatc
      901 atattaaagc aagatgtcgc taatctgggt cataaagacg acatcgtaaa agtgaaagac
      961 ggctatgcca acaactacct gatacctcag ggatttgcaa cattagcaac gccatctgca
     1021 aaaaaaatgc acagcgaaaa catgaagcaa cgtgcacata aggaagaaaa actccgcgcc
     1081 ggtgcacaga ctttcgccga caaacttaca ggttcgcgtt ttaaaattgc taccaaagtg
     1141 agctctaccg gaaaaatata cggttcggtg aacaccatta tgctctctga agcacttctg
     1201 gggtcaggtt ttgaagttga tcgcaaaaac atcaagatca aggaagagga agtgaaggaa
     1261 gtgggtgtat atcatgccag tgttaaactg tataaggata tcagagtgga gtttgaattc
     1321 gaagtagttg ccgaggatta accctgcaaa tataatgtaa aaggctgctc caaggcagcc
     1381 tttttatttt tagttcaatc gaaataatgc tgtaaggctt attttacagc aatcgtttca
     1441 atttcgacca gtgcaccaag tggtaaacct ttaactgcaa aggctgcacg tgccggaggg
     1501 ttctctggat agtagcgccc gtaaacttcg ttcattgctt taaagttttc catatcgctc
     1561 aataggcaag tcgatttaat cacattgctg aaagtgtatc ccgcttcatc gagaatggcg
     1621 ccaatgttct tcattacttg ttcggtttgt gcagtaattc catcagggat ctgaccattt
     1681 tcggggttta ccggtacctg acctgatata aacaacatac tattagcttc aactgcctga
     1741 ctgtaaggac caatagcctt aggtgcctta ttggttgtaa taattctttt cataatcgtt
     1801 atttatagat tttagtttgc atttctccgc gcagcaccat aagtccattc attgccaacg
     1861 catacatctc atcttctccg gcatacactt tcacaggggc aataaacgat accatatctt
     1921 cgataaagct gcagaattgt ttgttgtaag ctatacctcc ggtgagtaag atgcaatcaa
     1981 cttttccttt cagtactgcc cctgcagagc cgatttcctt ggctacctga tatgccattg
     2041 catcggagta gaactgggct tgtgtgtcgc ctgcctgaca gcgcttttca atttcgtatg
     2101 catcgttggt tccgacatat gctacaaatc ccccttgtcc ggtgatcatt ttctttactt
     2161 ctgcaggggt gtatttgccg ctgaaacaca actgagccag ttgccatgca ggtaatgtgc
     2221 cggaacgttc cggtgaaaaa ggtccttcgc cattcagggc attgttcaca tcaataacat
     2281 ttcctttttc gtgagctcca acagaaattc cgccacccag gtgaaccaca atcaaattga
     2341 gttcttcgta aactttgccg gaatcttttg catagcgtct tgcaatagct ttctgattga
     2401 gggcatggaa aatggaaaca cgtttgaaaa gaggatgtcc tgcaaccctt gcaatatctt
     2461 tcatctcgtc aacgaccaca gggtcagtga tgtaggcacg cacgttattc agactttttg
     2521 caatatcatg ggcaatcaaa cctcccagat tcgaagcatg ttctcccata ggagggttaa
     2581 acaggtcgtt tatcatggct tcattcacct cgaaaatacc cgatgtcacg gggtgaacaa
     2641 gaccgcctct gccaatgatg atgtcgatgc tgttgatatc gagcccccct gtgattaatt
     2701 ccttcaatac tgcttcttta cggaattcat actgggaagc aatatttttg aaatgtgcca
     2761 attcctcagt actgtgtttg atgtttttaa cgagaacttt ttcttcgttt tcgtacacag
     2821 caacttttgt ggaagttgag cccgggttga tggctaagat cctgtattgt ttcattttca
     2881 gagggttaac ttatttaagc attgctgccg ccaatgcaat cgacaacatt ttcgtttctt
     2941 cggaatctgc cctggaagta agcactatcg gacaacgtgc acctagaatt accgctgcca
     3001 gcaaagcacc gcccataaaa gcaaacgact tatacaacat attcccggca ttcaattcag
     3061 gtgcaaccag taagtcggca tcgccgccca catcggttat tatcttcttt tgctcgcatg
     3121 cttccttcga aactgcattg tcgagtgcca gaggtccatc gataatacag cctgtaatct
     3181 gtcctctacg attcatttgt gtaagcattg cagcgtgtat ggtgctttca atttttagat
     3241 taaccacttc aacaggaccc agtatggcaa ctttcggagt gtcgatacca atgccacgaa
     3301 gtgcttctac tgagttgttg ataatcgcca ccttctcgtt aaactccggg gctacattca
     3361 tagcagcgtc agtaacagct atcagtttat gataaaaatc cgactgaaaa attgcaagat
     3421 gcgagagtgt gtctcctacg cgtaagccat tttctttgtc tagaactgct ttaagtaaaa
     3481 tagcggtagt aaccataccc ttcattagta attcggcttt gccttcgcgc accaaattca
     3541 cggctttttt acatgccact acgtcgtttg gctcgtcaat aatctgatca tcagcaagtt
     3601 taaacccgat ttcattaagt atatttctgg tgatttcagc attcccaacc agaaggggtt
     3661 ttacaaagcc ttgttgtgta gcgtggtgta ctgcttcaag aacatgttta tcggctgaag
     3721 ccgctactgc aattgttcta acacctttag cctttgcaat gccaattaat tcattcagtt
     3781 tttttagtgc cattccgttc tttattaatt agttaatgct ttattttaag attaaagtta
     3841 aaaaattctg ataaagttaa aaaattctaa aaaaatgagt taaccccaag acaatattta
     3901 ggcggttttc ttaaaagaat tttaaaaaac tatcagcctg caatatcatg tttctatttg
     3961 actttcatgg cagatacgat cctcaacgtg tcgcgggcga taaccagttc ctcgttggtt
     4021 gtaactacca taattttaac ctttgaatcc ggagttgaga tggttccatc aattccacgg
     4081 gtattctcat tcaggttact atcgaaatgt gcacccagat atcccagatg gctcgcaatt
     4141 ctctcgcgaa caggccaagc attttcacca attccacctg tgaaaattat cagatcaata
     4201 ccattcattg cagcaacata tgatccgatg tacttgcgaa cgcggtaggc aaacatttcg
     4261 atggcaacgt cagcgcgttt atttcctttt ttaccggcct ccatcagatc gcgcatatcg
     4321 gaagagactc ctgaaaaccc ctgaagtccg ctttttttgt tcaccagatt gtttgccgat
     4381 tcaacgtcga tattatcagc tttcataata tgaaggattg ctcccagatc aaggtcccca
     4441 acgcgtgtac ccatcataag accttcgaca ggagtgaatc ccatactggt atctacagaa
     4501 atgcctttat caatggcaca gattgatgct ccgtttccga gatggcaggt gatgattttt
     4561 cgctccttaa tatcccagtt cagtgcttta catgcttttt cggccacgaa tttatgactt
     4621 gtaccatgga aaccatagcg tctgattttg tatttttcgt aatactcgta gggtagcgga
     4681 tagaaaaatg catactccgg catggtttga tgaaacgaag tatcgaatac tgccacctgt
     4741 ggaattcccg gtaacaaagt aatcatagca tcaatccctt taagattggc agggttatgc
     4801 aggggtgcca gactgcagca gttctcaata tttttacgtg cttcggtagt aatgaatgca
     4861 ctttggtcaa aatattctcc accatgagca acacggtgcc ccactgcgtc aatttcgtta
     4921 aggttgttga tgactccggt ttctggagca accagtatgt cgagaataat cttgatgcca
     4981 acggtgtgat caggtattgg ctggttcagt tcaagacttt tgcagtgagg tcccttgtga
     5041 gtgagaactc cctcgggtaa tccgattctt tcgaccaagc cttttgcaag cagttcggct
     5101 tctgcttgca tatcgaggag ctgatatttt attgaggagc tcccgcagtt caatacaagt
     5161 actttcatct ttttgtgttg atattgttta aaaaattatt aattgttcta ttttttagca
     5221 gctgcctgat ttgcagtaat ggcaataagg tttacaatgt cgctgactga gcagcccctt
     5281 gaaagatcgt tgatcggagc tgccataccc tgaagtacag gcccgattgc ttcagcattt
     5341 gcaagccgtt gtaccagttt atatgcaatg ttacctgttt caagagaagg gaaaaccagc
     5401 acattggcat gacctgcaat cttgcttccg ggtgcttttt tctggcctat agaagggatt
     5461 attgcagcat cggcctgcag ttccccttca atctgaagat cgggagccat ttcttttgca
     5521 aggcgcgttg cttctacaac cttatcgacc atggcatgcg aagcacttcc ttttgtcgaa
     5581 aaactgagca tggcaatacg gggctcaaaa cctgcaattg ctcgtgtggt taatccggtt
     5641 gctacggcaa tctgggcgag ttctgcagct gtaggatccg ggtgaactgc acaatcagcg
     5701 aaaatcataa tgccattctc cccgtactca gggttgttca aaatcataat aaatgcaccc
     5761 gaaactaccg aaatcccggg aagggtttta actatttgga atgcaggcct cagtacgtca
     5821 ccggtagcgt tatcggcacc ggcaacttcg ccgtccgcat cgccgcattt tatcatcagc
     5881 actcccagat acagcggatt ctcaacaaga ttggctgctt cctcaaggct aagacccttg
     5941 tttttgcgca gttcatacag ggtttgtatg taggtatctt ttttgtcgtg ttttttcgga
     6001 tcaacgatat acgcgttttt aatgtttttt aagccgaatt tatcagccat gctttcaatc
     6061 tctgccttgt taccgatgag aatgattgag gcaagttttt cagagattgc ctgatcggca
     6121 gctttaagag ttctttcttc tgtgccttct ggcagaacaa tgcgcattgt tttatttctt
     6181 gcgctttctt taatttgatt cagcagttcc attgcgtttg tatttatagt ttttaagctc
     6241 gtagaacatc caaaattaac aaatcactga ataattccta atgatgaaat ccttttttta
     6301 tgcgaaaaat ggtagtttca aaacattgtc ataaccgctt agggtaaaaa tgatattcat
     6361 ctgtgtttta ataattttca atataaaaaa cgctgaaatt aacaccggcg tattttcgca
     6421 actgtttgaa attctgtaat ttacttaaat cggtatcgcg tgaatgttcc attatcaacc
     6481 atccgttttc tcgtaggagt tgatttgaga aaaccaactc gtttattaat tggtaattgg
     6541 cttcctgata tggaggatca gcaaaaataa tatcgtatgt ctgtctgcaa tgcttcaaaa
     6601 acccatatac atcggtttta agcacttgta tcgaattaca atcaaaccga gctgcatagc
     6661 ttattatcga ttttacatat ttgtcgttga tttcgatggc tgtaatatca ctgcagcccc
     6721 ttgaaaaaag ctcaaaactt atgtttccgc ttcctgcaaa caagtcgagt gcgcgaactg
     6781 actcaaaatc gaaataattg ttgagcacat tgaacagtcc ttcctttgcg aaatcggtgg
     6841 taggccttgc atcaaagctt ttgggaggct caatatgccg tcctttcaat attcctgcaa
     6901 ctattcgcat tcaacaatat tcagaaagtt gtaaaacata tgtgccggaa cttcctgaaa
     6961 tcgatagcag taattgaaat gcgtatttct gccttcaaaa aaaacaaatt gcacaaactt
     7021 gcgaagttga ataatggcaa tactgtcttt ttcgataaaa ccggaaacag aacagtgggt
     7081 tttggcctga tcgagtttat attgattgaa aatactgagg agataatata caatgtcgtt
     7141 tgcactcttg tacctgaacg tgttgaacag caacagctta tcgttttcga gcaacatcac
     7201 ggaaaagtaa tcgccataca catcggtaaa tatcgtatgt aaaggtctgt cgttgttgag
     7261 cagatggcgt gtaaatccat aatcgacaaa cggtgctgac tgagcatacg tcaattttgc
     7321 tggaaatgca aaattcagat aattattcag cttctcagga attccgtaaa ccatatagtt
     7381 tctgctcgct ggaagataga tggcaagcgg tttccgcttt gttgcatcac caaaacttag
     7441 attgaatatt ttatcggtat cgttttcatt gaataaaacc gcaggaatta ttgtgcattc
     7501 cggtgtgcaa taaacgattc taacttttgc aaattcattg tttcttaggt cgcttttttc
     7561 ttcaaaaaag gtaatcagct ttgctgtatc gttaatttca ggaatgtggt aaataccaag
     7621 gtatttgttt gtattacgct ggtaatatga atatgaaagg ccatttgcag aaatctgaat
     7681 ggacaaatcc agagtatttg tttcagtgtt ttttagctca atatcttcaa tagcaaatgc
     7741 acccattcat aatgaaggaa taattattcc cagtttccgg cattattatt cgctgtttca
     7801 aggcttccga cttttagtac ggagtctttc tcttcgttga agttaataac atattgtctg
     7861 tcgagtcctt gaagaatgtc ccagttgttt gccttggctt cgaaaacttt taccagtact
     7921 tcagaacctg tcataacgct ggctgtatcc atacggaatt ttacttttgt accaacaggt
     7981 atccggcaaa tctcttcgat gggatagtcg atatgtttaa ataaagtgtc ctttatgttc
     8041 agataaacgg tatctctttt aatgataccc attttaagag cctgtaattc tgtaagtgaa
     8101 tccggcacag atccgatagc ctttacaact ggcattttac cgtttttaat aaagttgatc
     8161 aacgtatcga acgaaggggt ataacaccca taaatcgatt tgtaggcaac ctgcgcagta
     8221 cgaatatcaa tcagacggct tatagttttc tggtatctgt cagccctctg tttttcaaaa
     8281 tcgatgggct tacgtattcc tgcaacaata aaatatacca aaacaacggc ggcagccaca
     8341 agaacaagtt taattgaaat gttaagtatt ttcattttta caatattaat tttattcgac
     8401 caacaaaatt aatttttttt tatttcaatc atcataattt aattgcaatt tcgtgggatg
     8461 ataaaaaact tttttacacg tacagtaatt acaaacctgc agcatgaacc tacacaatcg
     8521 caattaaaag cattggacag tattttcgat tttctgagta atgggaacga aaagatggtt
     8581 tttatactgc ggggttatgc gggcaccgga aaaaccagcc ttttgagtgc tttaattcat
     8641 acgatacctg tttttaaaat gaattcggta ctgatggcac caacaggcag ggcggctaaa
     8701 gtactcactc aatactccgg aaagatggct tatacaatac ataagtgtat atacaggaag
     8761 aagaataaaa ctgagacggg tggatttgaa cttaatttta atagaaacag tgatacactt
     8821 tttattgtcg acgaagcatc gatgatatcc gatacccggc agaatggaaa tgtttttggg
     8881 tctggatatc tgttgcagga tttgattgat tttgtgttta gtggtaaaaa ctgccggctt
     8941 ctgctatgcg gcgacgatgc acagcttccg cctgtggaga tatcggaaag tcctgctttg
     9001 gataccactt atgtcagcga tttgggctgc acatgcatgt cggctcaaat gaacgatgtt
     9061 gtaagacagg ttttcgactc tggcattctt tttaacgcaa cagttatcag gaaactggta
     9121 cagcatcaga agacgctgat tcccgttctt tcaaccgcaa attataccga tatcgtaatt
     9181 atcgataatg cttgtctgac ggaaacgata gagatgtgtt attcccaata cggattgggt
     9241 caaaccaaaa ttatcacccg ttccaataag caggccctgc gttataatca aggcataagg
     9301 tcacgcattt tttcctacga atcggagctt gtttgtggcg atcagcttat ggtggtgaaa
     9361 aataattatt tctggctgcc cgacgattac ccggtagatt ttatagcaaa tggcgacatg
     9421 ttgcagataa aaaaaataag aaaaagtcag gaactgtacg actgtcgttt tattgatgca
     9481 acggttactc tttccgatat caacgacatt gaactggatg tgaagctgct tgctgatgct
     9541 atttcagtgg gaaatgcctc aatgcctcac gatttttaca caaagttgta tgcagatgtt
     9601 gaagccgact atgcgcatat cgacaagaaa aagaacaggg tggaggctgt gatgaacgat
     9661 ccgtatttca atgcattaca ggttaaatat gcatatgcag taacctgtca taaagcacag
     9721 ggaggacaat ggcaggcggt ttttatcgat ttgggatatt tcgaaaccct tgagcccgac
     9781 atagcattac tgcgttggtt atataccgcg tttacccgtg caactgaaaa attgtttctg
     9841 gtgaatttta aagcagaatt tttcaacgat aacccataga ctattgaaaa acatagttat
     9901 ttttagactt ttttaattca gcatgatcag catttgcatc ccgatatata atttcgatgt
     9961 cagaccgttg gtgggccaat tgtttaaaga aatccaatct ttaaataacg gctgcgaaat
    10021 tatttgcatc gatgatgatt cagatgatta cttcaaaaac attaacggga atttttgcga
    10081 taaatgcggt gaatacgtgc agttatacga aaacataggt cgggctaaaa tcagaaattt
    10141 gtttctcaag tacgcagcca atgattacct attgtttctt gactgtgatt ctattgttac
    10201 gaacgataat tttgttaaaa actatctgct gtgtttgtcg gaaaacccga gtgtggtttg
    10261 cggtgggaga gtttatccgc ccgaacgtcc tgagactcac tttctgcttc gctggaaata
    10321 tgggcataag cgtgaatcaa aacctgcatc attacgaaaa agaaagccgc ataaatcgtt
    10381 tatgaccaat aattttatca ttcacaagtc gattttacta agcattcagt tcgaagagcg
    10441 tatatcgaaa tatggacatg aagatacgtt atatggattc atgttgcagc agaatggaat
    10501 ccctgtggtt caaaccgata attcgattct caacggtgag ctggaaacta atgctgagtt
    10561 ccttgctaaa tcggagatgg cgctcgaaaa ccttgcattt attattgctc agcttaatga
    10621 aaacagggaa tttatttcga atgtaactat tcttaggtgg tattaccgtt tgaaagccat
    10681 taagttgctt tttgttataa gcctgatgcg tgtttttttt ctacggtgtt ttcacaaaag
    10741 actgatttcg ggaaaagcgt gtattcgtat tttcgatatg tacaagctga tactttttga
    10801 taagttttat aaaaagtttt tttcttccag ataattgcaa acatcaacta tcagttcttt
    10861 cgtagtgtta tacatattcg cgagcaatca ctttattttc atttgaatta ccggcttggg
    10921 gagatttgcc agataagcag taaggctgtc cccaatttca atttccccaa cacctgccgg
    10981 agttcctgtg tatatcagat caccggtctt taaggtcata tatctggaaa tgaacgcaat
    11041 aatctggtcg aagctaaata tcatttcagc actgttaccc gattgtacta gcatatcgtt
    11101 acgatagagc ctgaatggta ttgatgaaat atccgggata tcggttaacg aaaaaaacga
    11161 gcccaggcaa gctgaaccat cgaatgattt acttattgcc caaggcagtc ctgctttttt
    11221 acattcttgt tgaagatcgc gggctgtgaa atcaataccc aaagtaactg cgtcataata
    11281 cctgtgagca aatctttcct caatatattt cccaactttc gaaatgcgaa gcaccaattc
    11341 acattcatag tggatctcgt tgctgaattc agggatataa aaaggccggt ttctggtcag
    11401 tagacaggag tctgctttca ggaagaatac agggttttcc ggggcctccg atttcatttc
    11461 ggtaacatgt tcagaataat tccttccaat gcagattatt ttcatgcggt gtacttgcta
    11521 ttttgttttt gacacttctg ttgaatcaga tggtttattt taatgatttg aatcgcacat
    11581 atacacgggt aaaaaccttt ttagtgtaaa gaggaaagtc tccctgcatt acccatgaat
    11641 aataccccgg gtctttccgg aatacttctt cgagtagttg tcctttatat ttcccaaaat
    11701 taaccatctc gcgtcctttc tcatcgaaaa taagtctgcc tgcaaaatcg gccagttctc
    11761 cataggatga tatttctgca agtttcacaa catcgttggg taagtcattg tatttatcca
    11821 gctgactctt aagtacttca taagttgcaa gtgtatctcc taaagcagag tgcgcttcgc
    11881 tgtgttcctt gttacaataa aatttatagg ctgcgctcag tgttctgggt tcttttttca
    11941 tgaaaagtac ctgtacgtca acgaagttat gctttttcaa atctatttca atgtcagcac
    12001 gcaggaattc ttcaaccagc attggaatat cgaatttcag cgagttgtac cctgccaaat
    12061 cacagccttc gataaaagtt gctatttctt tggctgtact tttgaaagtg gggcaagtcg
    12121 ctacatcaac atctctgatg ccatgaattg ctgtcgtctc ttcagggatt ggtatttctg
    12181 ggttaaaccg atagcttttt gtttcttcgc gcccattggg tagcactttt atcatcgata
    12241 tttcaatgat tctgtcatta attatgttga gaccggtagt ttcaatgtcg aaaaaaatca
    12301 gtggtttttt taaatttaat tccatgtcgt taaaataaaa aggggcttgt agcccctgat
    12361 attactagta tagtttaatt ctaattatta atcatcattg gcattagcag catgagtata
    12421 tgctctgacg tgttttcgtt gtctttagga agtaccaatc ctgcccgggt cggatcgctt
    12481 agctcaatca ccacttcact tgtggatagg ttctgcagaa tatccatcag aaaaccggat
    12541 ttgaaaccaa tatccatttc atcaccctcg tacaaacaat ttatcatttc ataaccagaa
    12601 atggaatagt cgatatcctg agctgaaatg gtaagctgat tccctgtgat gtgtagcttg
    12661 acaaggttac ttgcctggtt tgagaaaatt gaaacacgct tcagggtgtt ataaaactca
    12721 actctgtcga cagtgagttt gtttgggtta ttcgatggaa ttacagcgct gtagttcgga
    12781 taagttcctt caacaagccg gcagactagc tgaaagtcct taaatgtgaa gaaagcattt
    12841 ttattgtcga actccagagt aacttcacct gatgcctttg gaaggatgtt tttcagaatt
    12901 gcagccggtt tttgcggcag aatgaatttg tcatttactt ccgctacaac gtcagtacgt
    12961 ttgtaacgta caagtttgtg agcatcggta gcaacaaaat ttaccccatc cgggctcatt
    13021 tcaaacaaaa taccattcat cacagggcgg agttcatcgt ttgctgtggc aaacagggtc
    13081 gaggtaattc cggagtaaag catttccggt ttcaggctga tatttgttga cttttcgcta
    13141 ttcatcaccg gaagttgggg gaagtcgtcg gcattaactc caatcagcga aaacttgcca
    13201 ttatcgctgt taatatcaat ttttaggctt tcgtcatcga cattaaaagt aagtggttgt
    13261 tcaggaaact ctttcagtac atcaagcaag cgctttgcat ctatacccag gcttccgtcg
    13321 ccgctggcag aatcaagctg caggtgagca gtaagagtcg attccagatc gcttgcagta
    13381 atatgcataa taccactttc gacacggatt aagaagttgt cgagtatctg aagggtgttt
    13441 tttgaactta ccaccttact tactatttgt aagcgactca acaggctggt gcttgaaata
    13501 acaaaattca tttttttaat gcttgttaat tatgtttaat tgcataaaga tactttgttt
    13561 aatgaaaaac aaaaatactg aatcaaaaat aatgtaatat tttttattaa cacaaaaatt
    13621 cttaggggtt cgctcctctg aaatcactga gtttttttgt cagaaggtca aaattgatgt
    13681 attgcagcag aaccagatct ttatcattat taattacacc atagaatctg tcgacatcat
    13741 tattgtagaa atttccctca tcatcgtaaa cgtttttgaa attggggcgc gaaaaggttt
    13801 taagtgtttt tttagttccc gatctatctt caacagtata aatgcacatt tcaggacttt
    13861 tcagaatgga atcaagtttc tgatattcag ttgtgccatt cagaaagctt tcgaacccta
    13921 cgaatttata acgcgatata aactctttta tcatcatcgg gtctgcctca gtaacaggct
    13981 ttccggtctc aagcgaaata aggctgtatg agttgttccc gttatttact gccatgaaag
    14041 aattctccgg gtcagcaggg aaaacaagac tcactgattt tatgtcggaa gcactatact
    14101 tgaacaatat acggtctttc cattcaaata aatctgttat gtagcgtgtt gaaagaaacc
    14161 ctgtaaaacc ggggatatgc atttcgaaag gtgcgctcga tccttcaagt atcatgtaag
    14221 tacctttgtt atcgggggta acacctccca cgtaataggt tttcaccagc gtattgtcct
    14281 gatatatttc aaccttggtc gagttggttg aaattgatcg gagcacgtaa tcaagcttcg
    14341 atttcggcac aggggcagca acttcaacat cttttattgt ttcaagcaga acatctacaa
    14401 aatcacggcg agcaatattg tctccattta ctgtccaatg gtcatttttt ctctccagta
    14461 atatggtgtt attttgtttg tcggccagaa atattttatt gatggaggcg gtatcgttta
    14521 ctgcaaagtc gcgtaaaact ttcagagtcc ctttaattct ttcaatacga taatgagttt
    14581 cgccgtagct ctcgtagctc acaatatggt atcttgtaaa aaacacaata atggcagcaa
    14641 ccagcaatac aataagaatt atcagtaata tcaggtttct tcgtttcatt tttttgtttt
    14701 atttcttcca tatttgtatt tcctgatgac aaccgtaaaa ataccggtaa gaatgatgat
    14761 tgcaataggt acagcagtat tgaaccaaac ccagaaaaca ccgtcctgtt taacttttgt
    14821 gcggtcaagt tcccgcattt tgatattacg cattctaagt gcaatcattt cttcgtcttc
    14881 gcacaagtaa tttatgcaat tgaccagaaa ttcgcggtta cccgggcaat attgttcctg
    14941 ataatatttg tcttcaccca gatagtaggg ttgcttctta tttcccagtg tttttacttc
    15001 gttcctaatc atatctccgt ccgaaatcac aatcattttg gttggtttac tgattggtgc
    15061 gaaattaatt tcattgctgt tttgaatttc cgggggaatc cggttattga aattcgattc
    15121 aaagcagcct tccatcaaaa ctgcaatagg tattcttggg ttattaaaag tggcagggtc
    15181 cggcctgtct tttataaggt caagacttat ctgcacaggg gccatcttcg attttgaata
    15241 ttgcgatgtt cgcagaagta tggtcttttt tacttgagta ttatcaccaa cagtatcgat
    15301 gctgcttata aattcggtcc ttatccggct aacatttctt gtaagtgggt gattggtaag
    15361 ggagtcaggt ataattacag ggaagaagta ccaaggaaca ggggcaaact gcggttgtcc
    15421 ccccgacgaa ttcatcatta taggtatgct gaggcatttc aaatcctgga ttaggtcggg
    15481 attgatgcgg actccgtagc ggaataactg atcatcaagg ttaatatcac gtataagagc
    15541 catgtcttcg ggcctcgacg ccaaactgtc catatctgcc gacatccatt ctacaagcca
    15601 cagcacttta ccgccattca taatatactg gtcaataatg aatttgtctt tctctcggaa
    15661 aacactgtcg ggttttgcaa tcacaatagc cctataaccg ttcagggaat tcagaatacc
    15721 attaatacta actctgtcga tttggtaata ttgcgataat tccatcataa tgtcgtaggt
    15781 ttggtattca tccaattcac catgtccttc gatgaatgca atcttttgct tagcaggtct
    15841 tgacaatagc cagatgccgt ggatgaagct gcgctcaagt tcactcactg ttttatcgaa
    15901 ttgttgctga tcgcctacac taaggtcatc aataaaaaaa tttacaggat attcctgtcc
    15961 attataaacg attatagctc ccgggaaaac aacctgctct gtgtactctg catctcccat
    16021 tatttgcagg gtttcgggca taagtccttt tttaataagc tgaatgaaaa tgtcacgttt
    16081 cttcgtttcg tctgcatcct taaatgggtc aataaactca tattcaatat tatcggaata
    16141 aaccctgaag tcatccaact tttcctgaac cgaccttttc aatcttttaa agccaagcgg
    16201 aaggttttca ccatcaagat atactttaat ataaaccaca tcatcgaggt tgtgaagcac
    16261 tttcttcgaa acctccgaga gggagtacct cttttctgca gttaaatcaa gcctataaaa
    16321 ccaaagatta ccgatgaaat ttattaaagc aacaataacc agtaccaaag tcaactgtat
    16381 taatccctgg cgtttgaggg ttctccgcgt aatacctgcc ggttgtttct gttttccgat
    16441 cataacaatt accattttct gctttcaagc gaaagcttgg tgaaaaaaac aaaaagggtg
    16501 atcacgctgt agaaatatag tatatctcgg ctgtcgataa ctccccgcga cattgaagtg
    16561 taatggtcgt ttatgccaag agcgattata aaatcctgaa ttgaaccatt ggtgcttaag
    16621 ctggcaagtt gagagaaacc caaatagaaa aaataacata aaattacgct gataaagaat
    16681 gaaataatct gattccgtgt caatgaagaa gcaaataaac cgatagaagc atatacggcg
    16741 gcaagcaaaa ataatccaaa ataagagcct gctatgccgc ctaagtctag atttcccgga
    16801 ggatcggcca aaaccaacaa tgagataagg taaatcaggg taggtagtag ggcaattgcg
    16861 gatagaataa gagctgcaat atattttgca ataacaatct ctaattccga aataggtttt
    16921 gtaagcagcc attccgatgt gcccgagcgt ttttcttctg caaaaaggcg catgcaaact
    16981 gcgggtatca ggaacagaaa tatccagggg gccagaataa acaggctgtc aagtccggca
    17041 tagcccacat cgagaatatt gaaatcggtg tgaaaaatcc agatgaacat gctgttgata
    17101 agtaaaaaca caacaacaac tatgtatccg ataatagaat tgaaaaactc acttatttct
    17161 ttccaaagaa gagcacccat ctttatcaat ttttctgacc acaaaattat ttttttatca
    17221 tgaataatga aacaagtgct gtaattaatt ctgtttttgt tattgcaacg tatttcaatg
    17281 accgtaaaac gaattttcag ggtgtttatt atgaattttg tgggaatatg aacttctgtg
    17341 tttaacggca gcaatacgat tataattagt caggccttat ttttataatg caaaaacatt
    17401 atttttgcag atgattaaaa catagtatta tgaaacgaga cgatttggtt tttgacatta
    17461 ttcgcaaaga aaaagaaaga cagatgcatg gcatcgagtt gattgcttcc gaaaattttg
    17521 ttagcgatca ggttcttgaa gcaatgggtt cagtaatgac aaataaatat gccgaaggct
    17581 atccgggcaa gcggtattat ggtggttgtc agtttgttga ccagactgaa cagctggcca
    17641 ttgaaagaat ttgtgaactc tacggagctg aatatgccaa tgtgcagcca cactctggag
    17701 cacaggctaa cgcttctgta tttcttgcat gccttaaccc cggagatacc tttatgggtc
    17761 ttgacctcgc tcatggagga cacctt
//
LOCUS       DAOOQP010000058        17181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4054_length_17181_cov_5.283487, whole genome shotgun sequence.
ACCESSION   DAOOQP010000058 DAOOQP010000000
VERSION     DAOOQP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_17181_cov_5.283487"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLK75_07450"
     CDS             <1..961
                     /locus_tag="PLK75_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY39121.1"
                     /translation="RNKQTKAVDAYVVHPQKLQNEISVAGTLLAFDEVELKNEVSGRI
                     VELNLPEGQFVKKGTLLVKLYDGDLQANLNKLNAQLSTQQQVYQRQAELFKVNGISRN
                     ELENAALNVSVLQAEIAGVEAQIRKTEILAPFDGIVGLRNVSIGAVVNTSTVLASLRS
                     SAKLKLDFYIPERYASAIVKGMKVWFTLFSSEVKYEATVLATETRIETGTRELKVRAV
                     TDNISEVLVPGSFATVYLGLGSNEKALLVPTQCIIPKEQTKRLIVARNGKAEFVTVTT
                     GVRQSNNIEILQGLSEGDTVITTGVLFLKPGSVIKYAHISDSL"
     gene            958..4020
                     /locus_tag="PLK75_07455"
     CDS             958..4020
                     /locus_tag="PLK75_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY39122.1"
                     /translation="MTLSDIALRRPVAAVVASLLIVLLGVVGYSFLGVRLYPAIDPPV
                     ITVQTNYTGANAEIIESQITEPLEKSLNGIEGVKSISSRSAVGSSNITVEFELGADLE
                     KAANDVRDKVSQAVRQLPQDIDAPPTVTKADANNEPIIMLSAQSSKMSATELSDYAEN
                     VLLEKLQTIPGVSSVAIYGQQKPAMRLWLDPVRLAANNLTAADIHAALLRENIEKPGG
                     KIRGYATEIIVKTYGRLETEQDFNNLIIKNEGTRIVRLSDVGEASIGAQNEESGLTLN
                     GITAVNLAVIPLPGANDIQIADEFYKRLQQIQENLPDGMELIVVRDKSIFVRQSVRDV
                     GETLLIAISLVVLIIFLFFRNWIIAIRPLIDIPVSLIGTFFVMYLFDYSVNVLTLLGI
                     VLATGLVVDDGIVVTENIFKRIEKGMDKWKAAFEGTREIFFAVISTSLTLAIVFIPVI
                     FLEGFTGRLFREFGIVVASAVLISAFVSLTLTPVLNVFLGGSASHHSKFYNASEPFFK
                     SLEKSYRRVLSYIFQVKWIAIIILLLCVAMITGLMFSLKSELAPLEDHSFIRLAVTTP
                     EGTDYEVTRNIMDDIAETISDSFPEAKNILVRSGGGFMSTGSNSGNIMVFLTDPEERD
                     LTQQQLLERLSSMFSGFAPARVIPSQEPTIATSASRGLPVQFVIQNLDFGKLKTVLPL
                     FLEEASVSPVFSNVDVDLKFNKPEVNIVVDRLKANSMGVNEKDVADVLSYALSGSRYD
                     YFLRNNKQYFVIGQVMRSNRDDPADIASLFVRSSTGNMIRLDNLVKMEESSNPPTLYH
                     FNRYKSATVSANLSEGYTLGEGIAEMQRIADKLLDSSFQTELSGSSRDFAESSSNISF
                     ALVLALLLIYLILAAQFESFRDPFIIMLTVPTAIAGALLSLHIFGHTLNIFSEIGMLL
                     LIGIVTKNGILIVDFANRKQREGLPRRVAAFEAATARFRPILMTSLATIFGAFPIALA
                     LGAGAQSRIPLGIVVVGGLLFSMILTLFVIPVMYVYMSGRKKQNTNQL"
     gene            4025..5329
                     /locus_tag="PLK75_07460"
     CDS             4025..5329
                     /locus_tag="PLK75_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY39123.1"
                     /translation="MKTRLIPVIAALFIGYSPIIAQDTLSLNAAIGIAIENNYRIIIA
                     GSEVEIAKISNTPGNAGMLPQLNLKGSGNWEYGNVYQRFSDGSENNFPSFSGSTVNAA
                     SELNWTIFDGGRMFATKHRLESNQDLAELSLQEEKNKIRYDVTAAYFDVVRNYEILHS
                     LNKILEIAQQRFELSEKAFNTGMLQKSDLLQASIDLNAVQKSMIEQEYLIKQSEEKLR
                     VLLCLPSVEFVLSDSLKTEYLPDFETLSQSLIAANTELMILQKQTEVASFSLKESRRN
                     IYPKIGLNAGYYLLRTVNSEGTIIENYSIGPQIGGSIVIPIFQSGEYRRKIATDKINL
                     ASAEYQYRYYHDLIGSELKVQYLEFVRLEKLIELEKDNNLLMSENIAISLHRLELGEA
                     ASTEIHQAQQQYLESCTNLINYRYNLKLSEARIQLLSGENIR"
     gene            complement(5341..9036)
                     /locus_tag="PLK75_07465"
     CDS             complement(5341..9036)
                     /locus_tag="PLK75_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39124.1"
                     /translation="MKKLLILTLFVIISGAIRAQIINTIAGNGTEGCSGDGEHALNAQ
                     FSAPMDIVTDASGNLYITDYNCQVIRKVNAAGIITTIAGTGVAGNSGDNGPAIDAQIN
                     HPKGIIIDIDGNIVFVDYGNQSVRRISLTTGIITLIASGLNGPLKLACGPSGHIYVAN
                     LNGVINKIDYTTGIVSTLYTSPANVLSVAVDGNDNVYFSTTSNLIYYYNQTSGISSLY
                     AGGGGNPNGENIPALSVAINYPYDLDIDNQGNLIYLDSYNHRIRKIDRNTNLVSTIAG
                     NGNGFYAGDGGPAINASFAYSEGIYVDHCNNLFLADYSADRIRKVTINYTPASTPSLA
                     AQAICSGGMATIEISGSLNDATQWKLYSFGCGSIVEGTTSGNTITVTPAENTLYYVRG
                     EGDCVSPGVCSNITIQVTNDPAFIAHPTGAFLCAGNTHTMSVSASGGTPELTYQWQYS
                     TNATDWSDISVANQNSYTTPELASTTYYRCVVSAIGNGCNEIISNMATVNVTPVPEND
                     LCINAIPVTSFPYTSELLNNNCSSSDIPPTSGCNGYSNNVWFMLSGTGNIMSVSTISQ
                     LTNLNTEIHIYTGECTHLDEVACNDDSGTGNQSYLEWCTLAEVDYFISVGSNDLIGEN
                     GSFIISISDYPLSVPEIINSSPITICPGGEISVSAIPGNNGNSVYWYSEECNNSIFVS
                     GNTLTGIPVNDISYFAQTRNTLCPESASECSQIDIVVADALTFLQQPSDETVCAGGSC
                     SLSVVAEGGTPSLTYQWQISADGISFEDILNAGAVEYYVSSINETKYFRCVITASGAG
                     CGQQISEVATVMVLPDPVFTLQPSGAVICSGDFASLTVAASGGIPSLSYQWQISEDGY
                     LWNNIDNEVESTYTTGIVTSTTYYRCVATTIGPGCNMAISNMAVINVTDLTAPAPENN
                     ELEIVYGECFVEITSQPVANDNCDGIILGVTENDLYYDVQGNYLIIWEYIDSYGNVSQ
                     QSQYVVVSDETGPVPNAEELEDVVGECSAEVSIVPQASDNCSGTIIATTQDPLLYESQ
                     GTFYITWVYNDGNGNTSYQVQTVVVEDVSLPEITCNGDAVVDAMPNHYYLISNNGFDP
                     VSVSDNCLVESVENDFNNTNTLFGEFLPEGTNTITWTVTDIGGNTESCAITITVVPYV
                     GINDLNTEQMTVFPNPCNGKFNVVLPENQNALKIIVTDYLGKTIFEKNLDDKNTFSID
                     MNLSEGVYLLHIYSTTGRYYGLISIQN"
     gene            complement(9180..12368)
                     /locus_tag="PLK75_07470"
     CDS             complement(9180..12368)
                     /locus_tag="PLK75_07470"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOY39125.1"
                     /translation="MNFSTYKKLDIIFGWLAFIVAAVVYLMTIEPTVSFWDCGEFISS
                     SYKLEVGHPPGAPFFMILARFFSLFASGPEQVALMVNSVSAIMSALTIAFLYWTIAYF
                     ARRMIVKENTITTGNAVAIIAASFVGAMAYTFSDTFWFSAVEGEVYATSSLFTALVFW
                     AILKWNNNIDSPHSLRWVILIALLVGLSIGVHLLNLLAIPAMVFVYYFSKYKPKFLGI
                     VISLLISLFIIASIMWGIIPGVAIVASKFELLYVNGFGLPYNSGVLVWAIITVICLGL
                     SIYFTQMNYRQIWAFIFSALGLILVGVPFMSGSIFISIVLVLAIGAGVFLIAKKKPAF
                     LNLIMTSVTMIIVGYSVFAMIVIRSSANPPMDQNNPENVFNLLYYLNREQYGYRPLFY
                     GEYYYAEPERDSDGQPVYKEGNPVYAQHNGRYDIVDYKPIPVYQGEDCTVFPRMYSSS
                     DDHIGAYESWGGLREGQKPEFANNLKFFFNYQVGWMYIRYFMWNFAGRQNDIQGHGNV
                     IHGNWKSGIDFLDTPRLGDQNKLPDYLKNNKANNSYYLLPLLLGIMGLIVQFSKNKKD
                     GWIVTFLFILTGLAIVVYLNQTPYQPRERDYAYAGSFYAFSIWIGLGVIFLYDLLKRF
                     LPAIAGAALAFLVCAPVPALMAKENWDDHDRSNRFIAHDFAANYLNSCEKNAILFTYG
                     DNDTFPIWYAQEVEGIRTDIKVCCLPYFASDWYIDQMKMASYESGPMPLRMTRDLYEP
                     GTRDIVYSEKVIFGVQEKNDYISLDSLLNYISNKKTGVLENDGKEYYLYHKNKIFMRV
                     DSAKVVENGQMKPPYDTLFDPIIKWDLKRSYLFKNELLTLDLLNANNWDRPIYFTSIG
                     STNTLNLDEYFMLEGFAYRLCPYNTGNKSGLIDSELMYNRLMNVMKWGNMNDPNIYID
                     YTIERTTKILRIRKTFSRLAIQLFREGKPDKAADVMKKCKTIMPYNIYTMTYFDIDFA
                     EAYYTIGDTVSGDEAILEVTKVASQELDFYFSLEDDLFQRMEMEVQMSLETMRRAAAV
                     MMRNNREALFEQLGSKTQNYFKMYEDRGGAVQ"
     gene            complement(12488..16006)
                     /locus_tag="PLK75_07475"
     CDS             complement(12488..16006)
                     /locus_tag="PLK75_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39126.1"
                     /translation="MKKILVLFFILSAFTITVLSQNNCGTAQVIENLPYYQSGLTTSG
                     TGNDYSPEDACSSDAMACEDYVFAFTPETDMTIRVNLLNTSITSEIPFGVTANIGLFI
                     TEGCPDETGSVCIASEDGLQENPGLDMISVTGGVTYYIIVSSEFYVLFIQQFATTVSF
                     DIAVEQIYSVDAATIGIDPLLSGCDLNSATITAQFKNTGLEDFSGIELSFQVGNLSPI
                     TENYNNTVEAGEEFEYTFAAEADISELGTHTIKVFCHLPGDEFSDNDYQITSVTNTPL
                     IATFPHNEDFESNEGFFYTEGTNSSWAYGAPDDSNPELVINTAASGDYMWCTNLSGNA
                     NTGEVSYLISPCFDVHTLLTPTVKFKLWIEPGILPGYGGTGTLEASVDGGNTYNITVA
                     TWNTSTGGWAENEFEIEELIGEENVRFRFSYTSGFLASEGMAIDDFMVKDEVLVDVSP
                     GTLTLPVGACGLSDQEFVKLYINNNGALPQSDIPITYSLDGGLSWLNVTETVPGPIPP
                     HDSVGFTFGALADLSEPGSYLLVIRTMLVGDEDMNNDTITYEIINTMVVSVIPYAESF
                     ETEDHGWIGSAGSSWARGIPGDTLTINHAYDGQYVIATNPLGNTNFNEISELVSPCFD
                     FTSLTGVRLSMKIWYETGFMPATIDLQGSVDGGSEWFEIETGWTGSSGGWITKSYYIP
                     EFAGLSDCKVRIVYTGQLLPSEGIAIDDVHFDPVFEKDLGVSNFLSPGNSCALGASEH
                     LTIEIVNYGTASQSNFPVQYSMNGGGTWITQNFIGSLAPQQTAQFVFSQSTVDLSQEN
                     TYTVLFRTQISGDMNSGNDDFATTIVHTQSIGSFPYDEVFETSDGGWVAYGTASSFEL
                     GTPAGTIINAAGEGNYSWVTNLDGNHNSGELSYLQGPCFDFSELTNPMLKAAVIYETS
                     MFMAGFYVEYSTDNGITWDTLPDGGSTGSWYGSSLIPGFGSTWSGSSGGWVQVSTNMP
                     LLAGLDNVNLRFVFNSGSFSFMQTEGVGIDDIHVYDCTNLPESSFDYELNGLTVQFTS
                     DVSDADSILWNFGDNEFLPSTSTEPNPSFTYTQEGYYLVTLTAVNECGSTVVESYIDV
                     TTSLFLANENKIRVFPNPTHGTIGIEAPEYIKSVTVSNIQGSVLYSFQGNGKNISFDL
                     NGLSAGMYILRINTDSECYVRPFIAE"
     gene            complement(16133..16543)
                     /locus_tag="PLK75_07480"
     CDS             complement(16133..16543)
                     /locus_tag="PLK75_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOY39127.1"
                     /translation="MKNLKINPDIGLLILRVSVAALMLLHGIPKLIYGVESIQQLLIS
                     KGLPGFAAYGVYIGEVVAPILLIAGFRARLASLVFVINMIVIIITAHSNEVFAIGKYG
                     GLQLELIYLYMTGALSIFFTGAGKLAISTRSKWD"
     gene            complement(16550..16978)
                     /locus_tag="PLK75_07485"
     CDS             complement(16550..16978)
                     /locus_tag="PLK75_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39128.1"
                     /translation="MKKINIFVIATLALIPLLKSCSDDPAMMSPESVNASIQESSWTI
                     SKFTEDNRDETTYFAGYSFEFGDNGLVTAVKQTNSFTGTYSSDSDDSTTKLYLDFGNQ
                     PPFDELNEDWEVLEKTDNKIRLQHVSGGDGSIDLLEFTKN"
BASE COUNT         5081 a         3501 c         3529 g         5070 t
ORIGIN      
        1 ccggaataaa caaaccaagg ccgtcgacgc ctacgttgta catccgcaaa agctacagaa
       61 cgaaattagt gttgcaggca ccttgcttgc ttttgacgag gtggaattga aaaacgaagt
      121 ttcgggccgc attgttgagt tgaatttacc tgaagggcaa ttcgtaaaaa agggaacatt
      181 gctggttaaa ctttatgacg gagaccttca ggcaaacctt aacaagctaa atgcacagct
      241 ctcaacacag caacaagttt atcaaaggca ggcagaattg tttaaagtga atggaatcag
      301 tcgcaatgaa ctcgaaaatg ctgcattgaa tgtaagtgta ttacaggccg aaatagccgg
      361 tgttgaggca cagatacgta aaacagaaat acttgcacca tttgatggca ttgttggctt
      421 gcgaaatgtt agcatcggtg cggttgttaa tacttcaacc gttctggcaa gtttgcgcag
      481 ttcagcaaag ttgaaacttg atttttatat tccggaaaga tacgcatcgg cgattgttaa
      541 aggcatgaaa gtttggttta ccttgttcag ttccgaagtg aagtacgaag ctacagtgct
      601 tgcaactgaa acacgcattg aaacgggaac acgcgagttg aaagtaaggg ctgtaacgga
      661 caatatttct gaggtgcttg tccctgggtc ttttgcaacc gtttacctgg gattgggaag
      721 caatgaaaaa gccttgcttg tacctacgca atgtattatt cccaaagaac agacgaaaag
      781 gttaattgtt gccaggaatg gcaaggctga attcgtaaca gtaacaacgg gtgtacgcca
      841 atcaaacaat atcgaaattt tgcaaggact ttctgaaggg gatacagtta ttacaacagg
      901 agttttgttt ctgaaaccgg gttctgtaat taaatatgct catataagcg attcgttatg
      961 actttatcgg atatagcatt acggcgccct gttgctgctg ttgtagcaag cctactcatt
     1021 gtactgcttg gagtagttgg atattctttt ctcggggtgc gcctttaccc tgcgattgac
     1081 cctccggtga ttacggttca gactaattat acaggggcta atgccgaaat tattgaatcg
     1141 caaattaccg aacctctcga gaaaagcctg aatggtattg agggtgtaaa atcaatctca
     1201 tcgcgctcag cagttggcag tagcaatatt actgtggaat ttgaactggg tgccgatttg
     1261 gaaaaagctg caaatgatgt cagagacaaa gtttcacagg cagtacgcca gctgcctcag
     1321 gatattgatg ccccacctac ggtaaccaag gccgatgcaa ataatgagcc tataatcatg
     1381 ttgtcggccc aaagttcaaa aatgagtgct actgaattga gcgattatgc cgaaaacgta
     1441 ttgctcgaga aacttcagac tataccgggt gtaagttcgg tggcaattta tggccaacaa
     1501 aagccggcta tgcgcctatg gcttgatccg gtaaggctcg ccgcaaacaa tcttactgct
     1561 gccgatattc atgctgcctt attacgcgag aacattgaaa aacccggagg gaaaatcagg
     1621 ggatacgcta ccgagattat tgtgaaaaca tatggacggc tcgaaacgga acaagatttc
     1681 aataatctga taattaaaaa cgaaggaacc cgaattgttc gactcagtga tgttggggaa
     1741 gcaagtatag gagcacagaa cgaggagtca gggcttaccc tcaatggaat tacagctgtt
     1801 aaccttgctg ttatacccct ccccggagct aacgatattc agattgccga tgagttttat
     1861 aaacgcttac agcaaattca ggaaaacctc cctgatggta tggagctgat tgtggttcgt
     1921 gacaaatcga tttttgtccg gcagtcggtt cgcgatgtag gtgaaacatt gctcattgcc
     1981 attagcctgg tggttttaat cattttcctt ttttttagga attggattat tgccatacgg
     2041 cctcttatcg atattcctgt ttcgcttata gggacattct ttgtaatgta tttgttcgat
     2101 tattcggtga atgtgttaac cttgctcgga atagtgcttg ctaccggatt ggtggtggat
     2161 gatggaattg tggtcaccga aaatattttt aagcgcattg agaaaggtat ggataagtgg
     2221 aaagcagctt tcgaaggtac ccgtgagata ttctttgctg tgatttcaac atcgcttact
     2281 ttggcaattg tgtttattcc ggttattttt cttgagggtt ttacgggaag gttattccgt
     2341 gaatttggta ttgttgtggc cagtgcagtg ttgatatccg catttgtttc actcacactt
     2401 acacctgttt taaatgtttt tctgggtggt tctgcatcgc atcattcgaa gttttataat
     2461 gcttccgagc cttttttcaa aagtcttgaa aagtcgtacc gaagggtttt aagctacatt
     2521 tttcaagtta aatggattgc cattataatt ttgcttttat gtgtggcaat gattacaggc
     2581 ctgatgtttt cgttgaaatc ggagctggca ccgcttgagg accacagctt cattcgactt
     2641 gctgttacca cacccgaagg taccgactat gaagttaccc gaaatattat ggatgatatt
     2701 gctgaaacaa tcagcgattc atttcctgaa gcaaaaaaca tattggtaag gtcaggtggt
     2761 ggtttcatgt cgacaggctc aaattctgga aacataatgg tatttcttac agatccggag
     2821 gaaagagacc taacgcaaca gcaattgctc gagcgcttat cgtcgatgtt ttcaggtttt
     2881 gcacctgcca gagttatccc aagccaggaa cccaccattg ctaccagtgc atcgcgtggc
     2941 ttaccggtac agtttgtaat acagaacctt gactttggta agctgaaaac cgtgctcccg
     3001 ctttttcttg aagaagcttc cgtcagtcct gtatttagta atgttgatgt tgatctgaaa
     3061 ttcaataaac ctgaggtcaa tatagttgtt gaccggctaa aagccaacag catgggagtt
     3121 aatgaaaaag atgttgctga tgtgctgagt tatgcactga gtggtagcag atacgattat
     3181 tttctgagaa ataataagca atatttcgta attggtcagg tgatgcgaag caatcgagat
     3241 gatccggctg atatagcatc tttgtttgta cgttcttcta cgggtaatat gatacggctc
     3301 gataatctgg taaagatgga agaaagcagc aacccaccaa cattatacca tttcaaccgc
     3361 tataaatcag ctactgtatc agcaaatctc tcagagggtt acacgctggg agaaggtatt
     3421 gccgaaatgc aacggatagc agacaaactt cttgatagct catttcagac agagttgtct
     3481 ggttcatcgc gcgattttgc agaaagcagt tccaatattt cttttgcttt ggttttggca
     3541 cttcttttaa tttatcttat tcttgcagca caatttgaga gttttcgcga tccattcatt
     3601 attatgctta ctgtgcccac tgctattgcg ggagcattgc tgtcgctaca tatttttgga
     3661 catacattaa atattttttc agagattgga atgctgcttc tcatcggaat tgttacaaaa
     3721 aacggaatcc tgattgttga ctttgccaac cgaaaacaaa gggaaggctt accaaggcgg
     3781 gttgctgctt tcgaagctgc tacagccagg ttccgaccaa tactgatgac atcgttagca
     3841 acaatttttg gagcattccc aatagcactt gctcttgggg ccggggccca aagccgcatt
     3901 ccattaggta ttgttgttgt ggggggactt ttgttttcca tgatcctaac cctgtttgta
     3961 attccggtga tgtatgtgta tatgtcgggc agaaagaaac aaaacaccaa tcagttgtaa
     4021 ttttatgaaa acacgattaa tccccgtaat agctgctctg ttcataggtt atagcccaat
     4081 aatagctcag gatacactaa gcctgaatgc ggccattggt attgccatag aaaataatta
     4141 ccgcataata atagcaggca gtgaagtaga aatcgcaaaa atatccaata ctcctggaaa
     4201 cgcaggaatg ttgccgcaac ttaatttaaa agggtcgggg aattgggagt atgggaatgt
     4261 ttaccaacgc ttttccgatg gaagcgagaa taattttcca tcattttcgg gcagtactgt
     4321 aaatgcagca agcgagttga attggacaat cttcgacggt ggccggatgt ttgccactaa
     4381 gcaccggctt gaatcgaacc aggatttggc tgaactaagt ctccaagaag aaaaaaataa
     4441 gattcgatac gatgttactg ctgcatactt cgacgttgtt agaaattacg aaattctgca
     4501 ttcactgaac aaaatactcg aaattgcaca acaacggttc gaactttccg aaaaagcctt
     4561 caatacaggt atgcttcaaa agtctgatct gctacaggca agcatcgact tgaatgcagt
     4621 tcaaaaaagc atgatagagc aagagtatct tattaaacaa tcggaagaaa aactgagagt
     4681 attactttgc ttaccttcag tagagtttgt gttaagcgac agcctgaaaa cagaatattt
     4741 acccgatttc gaaacgctta gccaaagtct tattgctgct aataccgagc taatgatact
     4801 tcagaagcag actgaggtgg cttcgttttc attaaaggaa agtcgcagaa atatttaccc
     4861 gaagattggc ctgaatgctg gttattattt gctaagaaca gtaaattctg aagggactat
     4921 aattgaaaac tattcaatag gtcctcaaat tggcggaagt atcgttatcc caatttttca
     4981 atcaggtgag tatcgcagaa aaatagctac agataaaata aatctggctt cagctgaata
     5041 tcagtatcgg tactaccacg atttaattgg ctctgagctg aaagtacagt accttgagtt
     5101 tgttcgcctt gaaaaactca ttgaactgga aaaagataat aatttgctca tgagtgagaa
     5161 tatagccatt agtttgcatc ggcttgaact gggggaagcc gcctctactg aaatacatca
     5221 ggcgcagcaa caatatctag aatcgtgcac aaaccttata aactacaggt ataatctcaa
     5281 attgtccgaa gccaggatac aacttttgtc aggtgaaaat attcgataag tgatttgatg
     5341 ttaattttga attgaaataa gtccgtaata acgacctgtt gtactgtaaa tatgaagtag
     5401 ataaactcct tcggaaaggt tcatatcaat cgagaatgtg tttttatcat ctaagttttt
     5461 ctcaaatatt gtttttccca agtaatcggt aactataatt ttaagggcat tctggttttc
     5521 gggtagcaca acattgaatt tcccattgca aggattcgga aaaaccgtca tttgttcggt
     5581 atttaaatca ttgataccaa catagggtac aacagtaatg gtaatagcgc acgactcggt
     5641 attccctccg atatcggtta cagtccatgt tattgtattc gtgccttctg gaaggaattc
     5701 gccgaataaa gtatttgtgt tgttgaaatc gttctcaaca ctctcaacca agcagttatc
     5761 gcttaccgaa acagggtcaa atccgttgtt actaataaga tagtaatgat ttggcatagc
     5821 atctaccact gcatccccat tacaagtgat ttcaggaagg ctaacatctt cgaccactac
     5881 agtctgcacc tgataacttg tgttgccgtt cccgtcattg tatacccagg ttatatagaa
     5941 ggttccctga ctttcatata gaagaggatc ctgtgtagtt gcaataatgg ttcctgaaca
     6001 gttatcggat gcctgaggaa ctatacttac ttcagcactg cattcgccaa caacatcttc
     6061 cagctcttct gcattaggta caggcccggt ttcatcagat acaacaacat attgcgattg
     6121 ttggctcaca tttccgtatg aatcgatgta ttcccatata attaagtaat ttccttgcac
     6181 atcgtaataa agatcatttt cggtaacacc aagtataatt ccatcacagt tatcgtttgc
     6241 aacaggctgg gaagtaattt ctacgaaaca ctcgccgtaa acaatttcaa gctcattatt
     6301 ttccggtgca ggagctgtca aatcggtcac attgattaca gccatgttgc ttattgccat
     6361 gttgcatccc ggcccaattg tagtggcaac gcatcgataa taggtagttg atgttactat
     6421 tcccgttgtg taggtactct caacttcatt gtcaatatta ttccacaaat agccgtcttc
     6481 cgaaatctgc cactgatacg aaagcgaagg gattccgcct gaagcagcaa cagtaaggct
     6541 tgcaaaatcg ccgctacaaa taactgctcc ggaaggttgc agggtaaata cagggtctgg
     6601 aagcaccatt actgtagcaa cttccgatat ctgctgaccg cagcctgctc cagaggctgt
     6661 aataacgcaa cggaaatatt tggtttcgtt gatagaggaa acgtaatatt ctacagcgcc
     6721 agcgttcaat atatcctcga aactgattcc atcagccgat atctgccatt gatatgtaag
     6781 ggagggcgtt cctccttcag cgacaacaga gagtgagcag gagcccccag cgcatactgt
     6841 ttcatcggaa ggttgttgca gaaatgtaag ggcatcggca acaacaatat ctatctggct
     6901 gcattccgaa gccgattcag ggcaaagtgt attgcgtgtt tgtgcaaaat acgatatatc
     6961 gttgaccgga attccggtga gtgtgttacc tgaaacaaaa atactgttgt tacattcttc
     7021 cgaataccag tatactgaat tgccgttatt accgggtatt gctgatacgc tgatttctcc
     7081 acccgggcaa attgttatag gagaggagtt gattatttca ggcactgaca aaggatagtc
     7141 gctgatgctg atgataaaac taccgttctc gccaattaaa tcattgctgc caacagaaat
     7201 gaaataatca acctcagcca gtgtacacca ttccagatat gactggttcc ctgttccgga
     7261 atcgtcgttg caggcaactt catcaagatg tgtgcattcc cctgtataaa tatgtatttc
     7321 ggtatttaaa ttggtaagct gactaattgt agaaacactc ataatattac cggttcctga
     7381 gagcataaac caaacattgt ttgagtaccc gttacatccc gatgttggag gaatatctga
     7441 cgatgagcaa ttattattca gtaattcgct tgtatagggg aagctggtaa caggaattgc
     7501 attgatacaa aggtcgtttt caggaacagg ggttacattt accgtagcca tattactgat
     7561 aatttcattg cacccgttcc caatggctga aacaacgcat cgataatagg ttgttgaggc
     7621 aagttcagga gttgtatatg aattttggtt tgctacagaa atatctgacc agtcggtcgc
     7681 attcgttgaa tattgccatt gataggtaag ctccggagtt ccaccggaag cagacacgct
     7741 cattgtatgg gtatttcctg cacacaagaa tgcaccggta ggatgtgcta taaatgcagg
     7801 gtcgtttgtt acctgaattg tgatattact gcaaactcct ggcgacacgc agtctccttc
     7861 tccccttacg taatacaggg tgttctctgc cggagttact gttatcgtat tgccggaagt
     7921 agtaccttca acgatcgatc cgcatccgaa tgaatacagt ttccattgtg tagcatcgtt
     7981 taaacttccc gaaatttcaa tggtagccat tcctccgctg caaattgcct gtgctgccag
     8041 gcttggcgtg cttgccggag tatagttaat cgtaactttg cggattcggt ctgccgaata
     8101 gtcagccaag aaaagattgt tgcagtggtc aacatatata ccttcggaat atgcaaaact
     8161 tgcattgatt gcaggtccac catcgccggc ataaaaccca ttcccatttc cggcaatagt
     8221 acttaccaga ttagtgtttc tatcaatttt tcttatgcga tgattgtatg aatctaaata
     8281 aatcaggttt ccctgattat caatgtcaag gtcgtaggga taattaattg caacagaaag
     8341 tgctggaatg ttttctccgt taggatttcc tccacctcct gcataaaggc ttgaaatgcc
     8401 tgaagtttga ttataataat aaattaagtt gctggttgta ctaaagtaaa cattatcgtt
     8461 cccatcaaca gctaccgata acacatttgc cggagatgtg tatagggttg aaacaattcc
     8521 tgttgtataa tctattttat taattacccc atttaagttc gctacataaa tatgccccga
     8581 gggaccgcaa gctaatttca acggaccatt cagtcctgat gcaatcaaag tgataatccc
     8641 tgttgtgaga cttatacgtc taacgctttg gtttccataa tctacgaaaa caatattgcc
     8701 gtctatatca ataataattc cttttgggtg gttaatttga gcatcaattg ctggaccgtt
     8761 atcaccacta ttccctgcaa cacctgtgcc tgcaatggtt gttattatac ctgcagcatt
     8821 aactttgcga attacttgac aattgtaatc agttatgtac aaattcccgg atgcatcggt
     8881 aacaatatcc atcggcgctg agaattgtgc atttaaggcg tgttcaccat ccccactgca
     8941 accttcagtt ccgttgccgg caatagtatt tataatttgc gccctgattg cacctgaaat
     9001 aattacaaac agagtaagaa tcaataactt tttcattaga aattttcttt aaagatacag
     9061 aaaatgtaat tttaacagaa ttagcttttc gatttttcga aaaaagagat aattcagaac
     9121 ctataaagat atttcaaaaa gttaatgaag tgaaatgaaa agagagtggg tgccatgctt
     9181 tattgtacgg ccccgcctct gtcttcatac attttaaagt aattttgtgt tttgctgcca
     9241 agttgttcaa acagtgcttc acggttgttc cgcatcatga cggccgcagc gcgacgcatg
     9301 gtttcaagcg acatttgaac ttccatttcc atcctttgga acaaatcgtc ttcaaggctg
     9361 aagtagaaat cgagctcttg cgaagcaact tttgtaactt caagaatagc ttcatctcct
     9421 gaaacagtgt caccaatagt gtaatacgct tctgcaaaat caatatcgaa atacgtcatt
     9481 gtatagatat tgtaaggcat tattgtttta cattttttca ttacatctgc tgctttgtct
     9541 ggcttccctt cccggaagag ctgaatggca agtcgtgaaa aggttttcct gatgcgaaga
     9601 attttggttg ttctttctat agtgtaatca atgtaaatat tgggatcatt catattaccc
     9661 catttcatta cgttcatcaa cctgttgtac atcagttcag aatcaattaa cccagatttg
     9721 tttccggtgt tgtaagggca taacctgtag gcaaatcctt ctaacatgaa atattcatca
     9781 aggttgagtg tattggtact tccgatagag gtaaaataaa tcggtctgtc ccaattgttc
     9841 gcattcagaa gatccaatgt aagtaattca tttttaaaca ggtacgaccg ttttaaatcc
     9901 cattttataa tcgggtcgaa aagcgtatcg taagggggct tcatctgtcc gttttcaacg
     9961 actttagcac tatcgacacg catgaatatt ttatttttgt gatagagata atactcttta
    10021 ccatcatttt ccagaacacc cgttttcttg tttgaaatat aattcagtaa gctatccagc
    10081 gagatatagt cgtttttctc ttggactccg aaaatcactt tttcggaata tacaatgtct
    10141 cttgtcccgg gctcataaag atctctggtc attctgagag gcattgggcc tgattcgtat
    10201 gaagccattt tcatctggtc gatataccag tcggaagcaa agtaaggcag acagcacacc
    10261 ttgatgtctg ttcttattcc ttcaacttcc tgagcgtacc atatggggaa agtgtcgttg
    10321 tcgccatagg taaaaagaat ggcatttttc tcacaggaat tcaggtaatt ggctgcgaaa
    10381 tcgtgtgcaa tgaaacggtt tgaccggtcg tgatcgtccc agttttcttt tgccattaga
    10441 gccggcacag gtgcacaaac caagaatgcc agcgcagcac cggcaatggc aggtaaaaag
    10501 cgttttaaca aatcatacag aaatattacc cctaagccta tccatatact gaatgcataa
    10561 aagcttcccg cataggcata atcacgctct cttggctggt atggggtctg attcagatat
    10621 accactatag ccaatccggt gagaataaac aggaatgtta caatccaccc atcttttttg
    10681 tttttcgaga actgaacaat gagccccatt atgccaagta acaagggcag caaataatac
    10741 gaattattag ctttattgtt tttcagataa tcgggcaact tattctgatc tccaaggcgt
    10801 ggtgtgtcga gaaagtcgat accgcttttc caatttccat ggattacatt accatgccct
    10861 tgtatgtcgt tttgtctgcc tgcaaaattc cacataaaat accgaatgta catccaaccg
    10921 acctgataat tgaaaaagaa tttaaggtta tttgcaaatt ccggcttttg tccttctcga
    10981 aggcctcccc agctctcgta agcaccaata tgatcgtcgg aactgctgta cattcgcgga
    11041 aatacagtac aatcttcgcc ttgataaaca ggaatgggtt tataatcaac aatatcgtat
    11101 ctgccgttat gctgtgcata tacagggttt ccttctttat aaaccggctg cccatcgcta
    11161 tcccgctcag gttcagcata gtagtactcg ccataaaaga gaggacggta gccatattgc
    11221 tcccgattca aatagtaaag taggttgaat acattttcag gattattttg atccatgggt
    11281 gggttcgcac tcgaccgtat tacaatcata gcaaatacgg agtagccaac aataatcatt
    11341 gttacagagg tcattatcag attaagaaag gccggtttct tctttgcaat taaaaatact
    11401 cctgccccaa tagcaagcac cagaacaata ctgataaata tgcttcctga cataaagggt
    11461 actccaacaa gtattaatcc caatgctgaa aaaatgaaag cccaaatttg cctgtaattc
    11521 atctgtgtga aataaatgct caaccccagg caaattacgg taattattgc ccaaacaagt
    11581 actccggaat tatatggaag tccgaatccg ttcacataaa gcagttcaaa ttttgatgca
    11641 acaatagcaa ctcctgggat aataccccac attatcgaag ctataataaa cagagaaatt
    11701 agtaacgata taacaatccc caagaatttt ggtttgtatt tgctgaaata ataaacaaaa
    11761 accattgctg ggattgcaag caggtttaac aggtgtacac caattgacaa accaacaagc
    11821 agtgcaatta aaataaccca acgcaacgaa tgtggagaat ctatattgtt gttccatttg
    11881 agtattgccc agaaaacgag tgctgtaaat agcgaagatg ttgcatacac ttctccttca
    11941 acagccgaaa accagaaagt atcggaaaaa gtatatgcca tggctccaac aaaacttgct
    12001 gcaataatgg caactgcatt acctgtggtg attgtattct cctttacaat catccggcgg
    12061 gcgaagtagg caattgtcca gtacaggaac gcaatagtta gtgcgctcat aattgccgag
    12121 acagagttca ccatgagtgc cacctgttca ggccccgacg caaagaggga gaaaaaacgt
    12181 gctaaaatca tgaaaaaggg agctcctgga gggtgcccta cttccagttt ataggatgaa
    12241 gaaataaatt cgccacaatc ccagaagctg acggtaggtt caattgtcat caggtataca
    12301 acagctgcaa caataaatgc aagccatccg aaaataatgt cgagtttttt ataagtactg
    12361 aaattcattc ttatagcttt taaataaatt caagagcggt aaaattaata taatattgtt
    12421 tagagagaaa aaaaacattc aaaatataaa aaaagcaggg ttttaaggtg ccctgctttt
    12481 ttctggttta ttctgctatg aatggtctta cgtagcactc tgaatcagta ttgattctga
    12541 gaatatacat gcctgcgctc agtccattta agtcaaacga aatatttttt ccattcccct
    12601 gaaagctata aagtacgctt ccctgaatgt tgcttacagt aactgatttt atgtactcag
    12661 gggcttcaat gccgattgta ccgtgagtgg gattcgggaa aacccttatt ttgttttcgt
    12721 tagcaaggaa taacgaagtt gtaacatcga tatatgattc aactacagta ctgccgcact
    12781 cgttcactgc agtaagtgta accaggtaat acccttcctg cgtgtaagta aaacttggat
    12841 tgggctccgt tgaggtagat ggtaaaaatt cgttgtcacc gaaattccaa agtattgaat
    12901 ctgcatcaga aacatcactt gtaaactgta cagtaagtcc attgagttcg taatcaaatg
    12961 aagattcggg cagattggtg caatcgtaaa catgaatatc atcgatgccg acgccctcgg
    13021 tttgcatgaa tgagaacgaa ccgctgttaa aaacaaagcg cagatttaca ttatcgagtc
    13081 ctgcaagcaa aggcatattt gtagaaacct gcacccatcc tccggagctt ccactccatg
    13141 tggaaccaaa accaggtatt aatgaactgc cgtaccatga gcccgttgag cctccatcag
    13201 gcagggtatc ccaggtaatt ccgttatccg tagagtattc aacgtaaaag cctgccataa
    13261 acatagatgt ttcataaatt accgcagctt taagcattgg atttgttaat tcgctgaaat
    13321 cgaagcaggg cccttgtaaa tagctcagct ctccagagtt atggttccca tcaagattgg
    13381 taacccatga atagttccct tcaccggctg cattaattat tgtacctgca ggagtcccca
    13441 attcaaatga tgaagctgta ccgtaagcaa cccaaccacc atcactggtt tcgaaaacct
    13501 catcatacgg aaaactacct atgctttggg tatgtactat agtggttgca aaatcatcat
    13561 tgccggaatt catgtctccc gaaatttgtg ttctgaaaag taccgtgtaa gtattttcct
    13621 gcgataaatc aaccgttgac tgactaaata caaattgtgc ggtttgttgt ggtgccaaag
    13681 atccgataaa attctgcgta atccatgttc cacctccgtt catggagtat tgaaccggaa
    13741 agttcgattg cgatgcagta ccgtaattta ctatttcgat ggtaagatgt tccgaagctc
    13801 ctaaagcgca gctattcccc ggagataaaa agtttgaaac gccgaggtct ttttcaaaga
    13861 caggatcaaa atgaacgtca tcaatagcaa tgccttcaga gggtaataac tgtccagtat
    13921 aaacaattcg gactttacaa tccgataatc ctgcaaattc aggaatatag tagcttttag
    13981 ttatccaacc accgctcgac cctgtccatc cggtttcaat ttcaaaccac tcactgccgc
    14041 cgtcaaccga cccttgcagg tcgatggttg caggcatgaa accggtttcg taccatattt
    14101 tcattgaaag ccttacaccg gtgagtgagg taaagtcgaa acagggactc accaattctg
    14161 atatttcatt gaagtttgta ttcccaaggg ggttagtagc aatcacatac tgtccgtcgt
    14221 aggcgtggtt tatagtaagg gtatcgccgg gtatgccgcg tgcccaggaa gagccggctg
    14281 aaccaatcca tccatggtct tcggtttcga agctttcagc ataggggatt actgaaacca
    14341 ccattgtgtt gataatttcg tatgttatcg tatcattatt catatcttca tcgccaacca
    14401 gcatggttct gataaccaac aaataacttc cgggttcact taaatcggcc aatgctccaa
    14461 aagtaaaacc tactgagtcg tgtggaggta ttggacctgg aacagtttct gtaacattga
    14521 gccaggaaag tccgccatct aaggagtagg taatgggaat atctgattgc ggcaatgctc
    14581 cattattatt aatgtacagt tttacgaatt cctgatcgct taatccacag gctccgactg
    14641 gaagggttaa agttccaggg ctaacatcaa ccagtacttc atcttttacc ataaaatcat
    14701 cgattgccat accttcgctg gcaagaaaac cagaagtgta agaaaaacga aatctaacgt
    14761 tttcttcgcc tattagctct tcaatttcaa actcattttc tgcccaccct cctgtagatg
    14821 tattccatgt ggcaacagta atattatacg tgtttcctcc gtcaacggaa gcttccagtg
    14881 ttccagttcc tccgtatcct ggaaggattc cgggttcaat ccagagttta aatttaactg
    14941 tgggtgtaag caatgtatga acatcgaagc agggtgaaat gagatacgaa acttcaccgg
    15001 tgttcgcatt tcccgaaaga ttggtgcacc acatgtaatc gcctgatgcg gctgtattta
    15061 tcaccaactc agggttgctg tcgtcgggtg caccgtaagc ccaagaacta tttgttcctt
    15121 ccgtgtaaaa gaacccttca ttactttcaa aatcttcatt atgtggaaaa gtggctatca
    15181 atggcgtatt tgtcacgctt gtaatttggt aatcgttatc gctgaattca tcacccggta
    15241 aatgacagaa caccttgatt gtatgtgtgc ccaattcgga aatatctgct tctgctgcaa
    15301 atgtgtattc aaactcttca ccggcttcaa ctgtattgtt atagttttcg gttatgggac
    15361 ttaaatttcc aacctgaaag cttagctcaa ttcctgaaaa atcttccaga cctgtgtttt
    15421 taaattgtgc agttattgtt gcactattca aatcgcaacc cgaaaggagc ggatcgattc
    15481 cgatagtggc agcatcaaca gagtaaattt gctcaaccgc aatgtcgaag cttaccgtgg
    15541 tagcgaattg ctggataaat aaaacataaa actcgcttga aacaataatg taataggtaa
    15601 ctccgccagt aacacttatc atatccagcc ccgggttctc ctgcaaaccg tcttcggagg
    15661 caatacatac cgatcctgtt tcatcgggac atccttcggt aataaacaag ccaatgttgg
    15721 cagttactcc gaaaggaatc tccgatgtaa tactcgtgtt cagtaaatta accctgattg
    15781 tcatatcagt ttcaggagta aaagcaaaaa catagtcttc gcatgccata gcatcgcttg
    15841 agcaggcatc ttcaggactg taatcattac ctgtgcctga agtagtcaaa cccgactgat
    15901 agtagggtag gttctcaata acttgtgcag ttccgcagtt gttttgcgat aatacggtaa
    15961 tcgtaaatgc actcagaata aaaaagagta ctaaaatttt tttcattatt cacatagttt
    16021 ttatgttagt gcaacaaatt taattttttc tttggaaagc aataacaatt taattaacaa
    16081 aaaaaaagcc tgactaaaaa tcagacctta aataaataca agcagaatca ggttaatccc
    16141 atttgcttcg tgttgatata gccaatttcc cggctccggt aaaaaaaatt gagagcgctc
    16201 cggtcatata caggtaaata agttctaatt gtaatccgcc atatttgcca atcgcaaata
    16261 cttcgttgct gtgcgcagtt attattatca caatcatatt tattacaaat actaacgaag
    16321 ccagtctggc acgaaaacct gcaataagca ggataggagc tacaacttca ccaatataga
    16381 ctccatatgc tgcaaaaccg ggaagccctt ttgaaattaa taactgttgg atagattcta
    16441 ctccataaat aagttttggg attccatgaa gtagcattaa agccgctaca cttacacgaa
    16501 gtataagtag ccctatatct gggttgattt taaggttttt cataactaac tagttctttg
    16561 tgaattcaag taaatcgata ctaccatcac caccgctaac atgttggagc ctgattttat
    16621 tatctgtttt ttcgagtact tcccagtctt cgtttagttc atcgaaagga ggctgattgc
    16681 cgaaatcaag atatagtttt gttgtgctgt cgtcgctatc tgaactgtaa gtgcctgtaa
    16741 aagagtttgt ttgttttact gcggtaacca gtccattatc gccaaactca aaagaatacc
    16801 ctgcaaaata ggttgtctca tcccggttgt cttcagtaaa tttacttatt gtccaggagg
    16861 actcctgaat tgatgcgtta acactttcgg ggctcatcat tgccggatcg tcggagcatg
    16921 acttcaataa tggtataagt gccaatgtgg caataacaaa tatgtttatt tttttcataa
    16981 tttttgtgtt ttgattacga gtaattaaca gttgaaggat tattttgttt aaaataattc
    17041 taaataacta ttttgaaata aatacgctga ctgttaaaat ataccgatac caaaactgtg
    17101 gtgcaatgca agaaatgaag aagttaaatt agtagtggtg cgttaatttt ttatttttag
    17161 tcataactca ttgatattaa t
//
LOCUS       DAOOQP010000059        17155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4058_length_17155_cov_5.630702, whole genome shotgun sequence.
ACCESSION   DAOOQP010000059 DAOOQP010000000
VERSION     DAOOQP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17155
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4058_length_17155_cov_5.630702"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..2146)
                     /locus_tag="PLK75_07490"
     CDS             complement(389..2146)
                     /locus_tag="PLK75_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOY39129.1"
                     /translation="MLGKIHIFVVTVLLTVISINSLHAQKKEVLIIHSYHQGLEWTDD
                     VTKALENVFLPLKDQYELHFEYMDSKRYDDKLFLDLATIYSRKYKSRNISAVIISDNA
                     ALDFIKEYRSIIAPKAPIIFCGINDYSPAMIEGVENITGITESSDYAANIELMIELHP
                     NLKKIIVVNDNKTLTSKLHKKLFSAAAEPYTAKVNFEYWEDLSIEEIEEGASKLKGNV
                     AIFLINFHLDKNERYIDYSEIANKTSKFNNVPVYGPFTFFLGKGIVGGMLTSGSLQGE
                     AAAQLTIRIMNGEKASDIPVITSNTNQYMFDYNKLKVFNININQLPKERVIINYEETG
                     DQFALNRKDYAIFFATDNYDDQSKWSNLKNPIKDATAIATILHDKYGFDTVIIRNPTL
                     LQIEENMKRLCQINFSDNDQMFMFFSGHGYFYDDTKEGFIVSKDSKEDYTTTYFPYSR
                     LRDMLTKQPCKHIFLVLDVCYSGTFFNTIASSKGENEAASKGVKFGDSDSNTASYIAS
                     SKYITRMVMTSSGKETVSDGIVHSPFATELLRILRTSNQSILTAYDLMQAVQNVPPFP
                     KIGAFEGNEPGSNFYFIMK"
     gene            complement(2492..8176)
                     /locus_tag="PLK75_07495"
     CDS             complement(2492..8176)
                     /locus_tag="PLK75_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39130.1"
                     /translation="MKNLLLFAITTLFCTVAFAQKQNIQLSSAVTQLDMKSVSAESFN
                     AEVNIKELSLENISNENGSFSTLEIEGTVAPSNVGRASLPVISKLIEVPFGAEIQVVI
                     NSYDEEVINLSDYGIERITPTQPSYSKSTPIEQQLFVIDNEYYNTDAYETSELISTEV
                     LGVMRGVRMGRIEIRPYRYNPVENTIIVYNNLAFTVNFIGADLATTEEMKARYYAHEF
                     DGSYGALINFQAPQAKDAFSNFNAPIKYIIVANTAFQATLQPFVLWKTKMGYEVIEHY
                     VATGTTNTTIKTYIEGLYDAANSSDPAPLYLLIIGDHSGTYSIPAFASKLTSSTYNQH
                     ITDVYFTTFDGTTDYLPDMYFGRISAESTTELQNALNKILPYEQYTIPSGTFLNNCQL
                     ISGVDASSAHIYGDSQILYGINNYYYEASDRFTNVWAFFYNNAGHAYNVLASSNTGAE
                     AAILSEISTGVGFSNYTAHCSYDGWADPKIARSDIASFNNQNKYPFMIGNCCQSFMFN
                     QSDAFGEMLLYTANEGAVGYIGASDYSYWSEDAWWGMGLTSLTLNTTNWASHTYANTG
                     LGAYDGLWHNHGEAFSDWYFSGRQIVHKGNLAVSASSSSIKQYYWEIYHLVGDPSLMP
                     YNTEPETLTISYGGLSVGSTSLTVTTEPYTYVAISQNGVLWDAEWSGSGTSVTLSFSA
                     IANANPIDIVATKQDRAAHIATITPVSTNPPVADFSGTPTTIMEGQSVAFTDLSQYAT
                     GWSWNFGDSQTATTQNPVHTYSTAGTYTVSLTANNNNGFDTETKVNYITVTPFTIVPV
                     AGFTADATSTCTGVVNFTNTSVDASSYLWDFGDGNTSTDENPTHVYTTSGTFTVTLTA
                     SNSYSSDTEIKPNYITVALPASPTTTGASNCGPASLTLSAAGTGTLQWYDAPTDGNLI
                     TTGTSYTQNFTTTTTYYVQSVIEPESQNGGKPDNSGTGNYFGNPDAIHGLYFNAYTDF
                     LLKSVKVYATGTKNRVINLKNSDNEIIATKTINIANGESRITLDFNVQAGNNYLLEGD
                     GDIDLYRNGGTTAPTLPYPYVITDVLSIHSNTADDNRYYYFFYDWEVVAGEPCISSRA
                     EVTASINTVPTAVTVSGGGTFCGGTATLTATGGTGGTIYWQGTTSSGTSTATASSSQT
                     VSTSGTYYFRARSSAGCWGPEGSATVTINAVPTAVTVSGGGTFCGGTATLTATGGTGG
                     TIYWQGTTSGGTSTATASSSQTVSTSGTYYFRARSSAGCWGPEGSATVAINPVPTAVI
                     VSGGGTQCGGVVDLIASGGSGGTIYWQGTTSGGTSTVTPTTAQSVTESGTYYFRARSS
                     AGCWGPEGSATVTINAVPTAVTVSGGGNYCGTNATLTATGGTGGTIYWQGTTSGGTST
                     TTASASQTVSGSGTYYFRARSAEGCWGPEGSATVSINPAVTLSLSMTQESTTGASDGT
                     ATATVGGGTAPFSYTWSPAGSGASLTGLSAGQYCVTVYDNAGCSTAGCITVTTANQPN
                     PPVAQFTSGTAFGCGTLTIQFTDQSTNNPDEWLWQFGDGATSDLQNPVHTYSNPGQYT
                     VSLTVGNDDGGDEVVMNDYITVWALPTVSLSMTQESSEGAGDGTITAEVSGGTPPYYY
                     EWSGPGSGPMITGLTAGEYCVTVYDYNACSASVCITVTVSSGQNDPVPVFDTDNALGC
                     APLSVSFTDMSTNTPTSWLWQFGDGSSSDQQNPEHIYAGAGVYSVSLTVTNEYGENTL
                     VMNGLITVYPVPSLSVTVANASGPDVADGSATVSIEGGTAPYTIYWSNSTSGNTMINV
                     LPGNYSVMVVDDNGCVATTPFVIGWNVSVEPVVTAGMKIYPNPTDTYLVVENSGFVAD
                     ELAISDVLGQVVLHIKPVSGTTRIDVSKLSPGVYFVSTKNNAKKFVQKFVVR"
     gene            complement(8343..8768)
                     /locus_tag="PLK75_07500"
     CDS             complement(8343..8768)
                     /locus_tag="PLK75_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39131.1"
                     /translation="MRKLFILIIIVSTATLLSCRDQIYYTGDDVNCYECYTDTLETGY
                     LIIEFTLNDENSEVPITVFSGEVDASPIVFSAPCDTSALILEVPMNAYYSVKAAYKNG
                     NDSIYVIDGGRLEAKLVTGICDVDCWVVQGGEYNVSLTY"
     gene            complement(8765..10165)
                     /locus_tag="PLK75_07505"
     CDS             complement(8765..10165)
                     /locus_tag="PLK75_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOY39132.1"
                     /translation="MIRRFIFLAIMCLWGSNLFAQYTKTDTIAADAYDFWMAADTGNF
                     EMVKLCVEKGIFVDVAYSDNVTPLMLASQSGHYEIVQYLIGKGANVNAYSESERVSAL
                     ISAVKNNDMSVAEFLIRKYARLQHTDILGRQALHHAVISGFYNMTDMLLYYDAPVNEK
                     DSLGCSPAYYAVVNGDTAMLQLLMKYGADITERNSNGESFVHIAVQYNVTDMAAFLIH
                     EGLVVTDISNSGTSLIDEALVNGNQVLIKLLLDHGAVLNDSITPYYNSMNLAMLSGNR
                     DIKRMVRSKGIKPMVKPYFQQLGVSLLFPFNLDDVFVGIGLDLYDTRYGFQLSGNAWV
                     RPGARRVWLQHDEGVYYQYWEKRWGIAVGLKKHFRIPDRPEFPLHLTLGVDEVFTGGS
                     YKATDKKNIPFLSLSPNAGLYIRFSSKAGMSIGYSYLDLGNKNISPHRVSIGIRGAFG
                     IKSPDSVNANRYIIKE"
     gene            10248..10320
                     /locus_tag="PLK75_07510"
     tRNA            10248..10320
                     /locus_tag="PLK75_07510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10281..10283,aa:Arg,seq:ccg)
     gene            complement(10598..11299)
                     /locus_tag="PLK75_07515"
     CDS             complement(10598..11299)
                     /locus_tag="PLK75_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY39133.1"
                     /translation="MKQEKIYKIILVDDDEDILEFLDYNFTKNGFSTKVLNNSDSVLP
                     MVQNFFPDAIILDVMMPGTDGIELCENIRNSLPEQNPLIIMLTARGEDYSQIAGLEAG
                     ADDYIIKPINPKVLITKVRSLLKRRETVVSSTIKNGQVENISTQNLIIDRERYEITEG
                     GKLFHLPRKEFELIELLASKPGKVFSRSEIYQKVWGDTFVCDRTIDVHIRKLREKFGD
                     DFIKTIKGVGYKINE"
     gene            complement(11442..14333)
                     /locus_tag="PLK75_07520"
     CDS             complement(11442..14333)
                     /locus_tag="PLK75_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39134.1"
                     /translation="MMKNCVALIFLLFALITASAQDGFVRGSVFDAQNGESLPAVAIV
                     VKGTTLGTTTDLDGKFSLQMAPGKYELEISFISYQTISLTQVEVKSGQIVSLGDLLMK
                     EEGILLQEVTIAASEVRNNDNALIAMKRKSVTMLDGVSSVGIRKTGDSDVASSMKRVS
                     GVSVNGGKYVFVRGLGDRYTKTVLNGLEIPGLDPDRNTVQMDIFPSNIVDNIMVSKTF
                     SAELPADFTGGIINIVTKDFPETRQSGFNFGLAYNLGSHFVDNFVGYQGGRTDFLGFD
                     DGSRAIPATSNIPFFAEVVGNTESEKAQRYKEILSAFNPTMAASRQMNLCDFSMGANL
                     GNQIQKKNFTLGYTTAFSYKISSDYFENAEYGRYGLAGDKSVFELDRREYQLGDFGVK
                     TVFLNFLGGIAVKTKNSKYRINLMHLQNGESKAGIFDYEKSNLGTNFSGFIHTLDYSQ
                     RSISNIIIDGKHNFGDSQWNVEWKLSPTLSVIYDPDVRFTRYEDREGVYSISTESGFP
                     ERIWRKLLEYNISTLANASKGFMLFGNKATLGFGGAFTYKQRDFVIRNFAINIRGVDL
                     SGNPNEIFDTENIWPMNGIATLGTTYETPFIPVNPNQYSSNIINAAGYVSLEFSILTR
                     LKTTLGLRTENYTQRYTGRDQMGYHILKNDVVLSDMGFFPAVNLIFTISDNQNLRAAF
                     SKTIARPSFKELSYAEIFDPITGRTFIGGLFRDANDLAGVEYWDGNLISTDIFNYDFR
                     WELFGAEQQLLSVSAFYKKFINPIEIVQFATQVGAFQPRNVGDGQVAGAELEFRKNLK
                     FITPALKCFSVNTNITYTLSRITLSPTELASRTENARIGQVIDNHRAMAGQSPYIVNA
                     GISYKAPEDKIGGIEAGVYYNVQGRTLLYAGIADRPDIWSKPFHSLNINANLNVGRQK
                     NIQISFKIDNILNSKQEAVFKSYMAEDRYFSKLSAGRLFQLKLAINFNK"
     gene            complement(14421..15626)
                     /locus_tag="PLK75_07525"
     CDS             complement(14421..15626)
                     /locus_tag="PLK75_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39135.1"
                     /translation="MKKLFYLMMILAATSVVLTSCDPEDDIPDDETITITGNITANTI
                     WETGNTYILGGRITVVSGITLTIEPGVIVKGQAGTGSNATALIIARGATINAQGTEAQ
                     PIIFTSVADEIEPGMIASPNLDTDLNGLWGGLIILGNAPISASGESVQIEGIPASDQN
                     GLYGGNNATDNSGIIRYVSIRHGGANIGEGNEINGLTLGGVGSGTTVEYVEVVANQDD
                     GVEFFGGTVNVNNLIIWNPCDDGVDTDQSWAGTLDNFIVIAGAETDHTLEIDGPEGSM
                     LAAHTLENGSIKGSESAELGDFRSGARGTFQNLHFFNFPDPATTDGRGDLSLSGDATL
                     ANFASGVLTFSNLQVTPATGVAVNSIFKNGTDVHASSVNAGANTVGADKTKFASWTWV
                     STNNELGDF"
     gene            complement(15889..16479)
                     /locus_tag="PLK75_07530"
     CDS             complement(15889..16479)
                     /locus_tag="PLK75_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39136.1"
                     /translation="MGNKTLLLFAFIGIIFMVSCRNQTHCPEFSGDDMEHFPYRSGDT
                     LCFVNADSEYLKLVVPEISVSDSYCYECNDLNGICNCERYAEARFSVVPEGLKPVLLK
                     LVIFSSNQSYRYYYSVYGFEFELDFENEAPYVYMMPEFDYLGNYVVSDSMFTKVYRAI
                     NLNYPESNVQHVFFSKNAGIIRIDEKQNGNIWQLKP"
     gene            complement(16555..17034)
                     /locus_tag="PLK75_07535"
     CDS             complement(16555..17034)
                     /locus_tag="PLK75_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOY39137.1"
                     /translation="MKKLALFIGFAFISMIALQSVNAMPTKGGNDKEVTLKVNMNCQA
                     CATKVEKQLAFTKGVRAVKADFEKDVVIVVYNSTKTDPNKLIASLKEIEYDAVVYDAN
                     AKQCPGNHSGCPNKKASSGDAGSGCPGSKTSNEAKPAGCAGQHNSGEGKASGCGGQK"
BASE COUNT         4749 a         3644 c         3596 g         5166 t
ORIGIN      
        1 cgatgcaaat ttatttgcaa tcgctaaaac aataaaaacc aaacctgcaa agcaggtttt
       61 ttcagatggt tgaatctcca cccttacggg atcacgagcg cagcgagtaa tccttaaacc
      121 caaaatcagc aatagggaat ttaacaaata tattttattg attatcaaca aataagctcg
      181 tgatttttct aatgcgtagt attagagaat tttcactgta aaattcaatg ataccatttg
      241 agcgcaagag ttgtatgata atcgcgaaag tgtttttagt tcctattgcg taatttgggt
      301 taaacgatat ctattcagtg ttattgaaag ccatccccat ccgagtcctc gttaagtgac
      361 tcagatggtt aaaaaaagga gcataaagtt atttcatgat gaaataaaaa ttgcttcctg
      421 gttcattccc ttcgaaagcc ccaattttgg ggaaaggagg tacattttga acagcttgca
      481 taagatcata cgctgttaaa atagattgat ttgatgttct taatattcta agtaattcag
      541 tggcaaaagg cgaatgaaca ataccatcgg aaacggtttc ctttccgctt gacgtcataa
      601 ccatacgagt tatatatttt gaagaagcta tataactggc agtattggag tctgaatcac
      661 cgaacttgac cccttttgat gcagcttcat tttctccttt tgaggaggca atcgtgttga
      721 aaaatgttcc actgtaacat acatcaagca caagaaatat atgtttgcat ggctgtttcg
      781 taagcatgtc tcttagcctt gagtaaggga aataggttgt tgtgtagtct tctttggagt
      841 ccttcgaaac aatgaaccct tcttttgtat cgtcatagaa atagccatga cccgaaaaga
      901 acataaacat ttgatcatta tccgaaaagt tgatctgaca tagccttttc atattttctt
      961 cgatttgcaa cagcgttgga tttctaatga tcaccgtatc aaaaccgtac ttatcgtgaa
     1021 gaattgttgc tatggcagta gcgtctttga tggggttttt cagatttgac cactttgact
     1081 gatcatcata gttgtcagtt gcaaagaata ttgcataatc tttacgattc agggcaaatt
     1141 ggtctccggt ttcctcgtaa ttaatgataa ctctttcttt tggcaattga ttgatgttga
     1201 tgttaaaaac tttcagtttg ttgtaatcaa acatatactg gtttgtattt gaagtgatta
     1261 caggaatatc agaggctttt tcgccattca taatccttat tgtgagttgg gctgctgcct
     1321 ccccttgaag gctaccactg gttaacattc caccaacaat acccttgcct agaaaaaagg
     1381 tgaaaggacc atatacagga acattgttaa actttgatgt tttgtttgct atttcgctat
     1441 agtcaatata gcgttcattt ttatcaagat gaaaatttat aagaaatatt gcaacattcc
     1501 ccttcaattt tgaagcacct tcttcaattt cttcaataga caagtcttcc caatactcaa
     1561 aattcacttt tgctgtatag ggttctgcag ctgctgaaaa gagtttttta tgtaatttag
     1621 aagtaagggt tttattgtca tttacaacta ttattttctt taaattggga tgcaattcaa
     1681 tcatcaactc tatgtttgcg gcataatcag aactttcggt aattccggta atattttcca
     1741 caccttcaat cattgccggt gaataatcat taattccgca gaatataatg ggtgcttttg
     1801 gggcgatgat actacgatat tctttaatga aatcaagcgc tgcgttatcc gaaatgatca
     1861 ctgctgatat atttcttgat ttatattttc tgctgtaaat tgtggcaaga tcaaggaata
     1921 atttatcatc gtatcttttt gaatccatat attcaaaatg caactcatac tgatccttta
     1981 aaggtaaaaa tacattttct aatgccttag taacatcatc ggtccattca aggccttggt
     2041 gataggagtg aattattaat acttctttct tttgggcatg caagctattt attgaaatta
     2101 cagtaagtaa taccgtaaca acaaaaatat gtattttacc tagcataatg cttatagttt
     2161 ataattctgc ataaaagtag ttaaaatcaa tagtataatg aatttatttt ttactttaat
     2221 gtgatatgct tatactgcat tcgaaaaagg attaaacatc agatatctat ttgttatcgc
     2281 ctgcatagta agtgtcttta tcattggtga aacttgcaac aggctgtttg gtgcatgaac
     2341 ttgcaataaa agcaattcca ataataaaaa taataattct tctcattacc tggatagtat
     2401 atagaggaat acaaatataa ataattatca ggtttcaaac aaaagaggct gcctccttag
     2461 aaacagcctc ttttaggaaa atagttccgg gttatcgcac tacaaacttc tgaacaaatt
     2521 tctttgcatt gttcttagta cttacaaaat atacacccgg gctgagtttc gaaacgtcga
     2581 tacgtgttgt gcccgatacc ggttttatgt gtagtacaac ctgaccgaga acatcggaaa
     2641 tggcaagctc atcggcgaca aaacctgagt tttcaacaac aagatatgtg tccgtaggat
     2701 tgggatagat tttcattccg gcagttacca ccggctcaac cgaaacattc catcctatca
     2761 caaacggggt tgttgcaacg catccgttgt catcgacaac cattactgaa taattgccgg
     2821 ggagaacatt aatcatagta ttacccgaag tactgttcga ccagtagatt gtgtatgggg
     2881 cagtgcctcc ttcaatgctt actgtggcag aaccgtcggc aacatcaggt ccgctggcat
     2941 ttgccacggt aaccgataat gacggaaccg gatatacagt gatcagtccg ttcattacaa
     3001 gggtgttttc accatattca ttggtaacgg taaggcttac tgaatacact ccagcgcctg
     3061 cataaatgtg ctcagggttc tgctggtcgg aactgctgcc atctccaaac tgccatagcc
     3121 acgatgtggg ggtgtttgtg ctcatatcgg tgaacgatac gctgagtggt gcacatccca
     3181 aagcattgtc ggtatcaaaa acaggtacag gatcattttg tcccgacgac accgttacgg
     3241 tgatacatac tgatgcagag catgcattgt aatcgtacac ggttacgcaa tattcaccgg
     3301 cagtaagccc cgttatcatt ggtcctgaac ccggtcccga ccattcatag taataaggtg
     3361 gtgttccgcc tgaaacttcg gcagttatgg ttccgtctcc ggctccttcg ctgctttcct
     3421 gagtcatact taatgaaaca gtgggtaaag cccaaacggt gatataatca ttcatcacta
     3481 cttcgtcgcc gccatcgtcg ttgcctacgg ttaacgacac agtatattga cccggatttg
     3541 agtaggtatg aacaggattt tgcagatcgg aagtggctcc atcgccaaac tgccatagcc
     3601 attcatcggg gttattggta ctctgatcgg taaactggat ggttagggtt ccacaaccaa
     3661 aagcagtgcc tgaagtgaac tgtgcaacag gagggttggg ttggttggct gttgtaacag
     3721 taatacagcc cgcagtgctg caaccggcat tgtcgtaaac ggttacacaa tactgtcctg
     3781 cacttaatcc ggtaagcgat gcgcctgatc ctgcagggct ccatgtgtac gaaaatggag
     3841 cggttccacc accgacagtt gctgtggctg taccatccga tgcaccggta gtactttcct
     3901 gagtcatcga caacgacagg gtaactgcgg ggttaatcga aacagtggca cttccttccg
     3961 gtccccagca tccttcagca gaacgcgccc tgaaataata ggttccgctg ccacttacgg
     4021 tctgcgaagc agaagccgtg gttgtgctgg ttccaccgct ggttgttccc tgccagtaga
     4081 tggttccgcc ggttccgccg gtagcggtaa gggtggcatt tgttccacaa taatttccgc
     4141 cacccgaaac ggtaacggct gtaggaacag cattaatggt aacggttgcg cttccttccg
     4201 gtccccagca tcctgccgag gagcgtgctc tgaagtaata tgttcccgat tcggtaactg
     4261 attgtgctgt tgttggagtt actgtactgg tgccaccgct ggtagttccc tgccagtaaa
     4321 tcgttccacc agaaccaccg cttgcgatga ggtcaactac gccaccgcac tgtgtgccac
     4381 ctccactaac aatcactgct gttggtacag gattaatagc taccgtggca cttccttccg
     4441 gtccccagca tcctgccgag gaacgtgctc tgaagtaata cgttccgctg gttgaaaccg
     4501 tttgtgaact cgaagctgta gcagtactag taccaccgct ggtggttccc tgccagtaga
     4561 ttgttccgcc ggttccgccg gtagcggtta gtgtagctgt gccgccgcag aaagtacctc
     4621 cgccgcttac ggtaacggct gtgggaacgg cgttaatcgt aacggttgcg cttccttccg
     4681 gtccccagca tcctgccgag gagcgtgctc tgaagtaata ggtaccgctg gttgaaaccg
     4741 tttgtgaact tgaggccgta gcagtgcttg taccactgct ggttgttccc tgccagtaga
     4801 tggttccgcc ggttccgccg gtagcagtta atgtagctgt tccgccgcag aacgtacctc
     4861 cgccgctaac agttaccgct gtgggaacgg tgttaatgct tgctgtaact tcagcacggg
     4921 aactgataca aggctctccg gctacaactt cccaatcgta gaagaaataa taatatctgt
     4981 tatcatctgc ggtattacta tgaatactta aaacatcggt tataacataa ggatagggta
     5041 aggtcggagc cgttgtacca ccattccgat ataaatcaat gtcaccatca ccttccagca
     5101 gataattatt tcccgcttga acattaaaat caagagtaat gcgactttct ccattagcaa
     5161 tattgattgt ctttgttgca ataatttcat tatccgaatt tttcagatta ataacacggt
     5221 ttttagttcc cgtggcgtaa actttcaccg attttaatag gaaatcggta taggcattaa
     5281 aatataaacc atgaatggcg tcgggattac caaaataatt ccctgtaccg gaattatccg
     5341 gtttgccgcc gttttgcgat tcaggttcaa ttaccgattg cacataatac gttgtggttg
     5401 tcgtaaaatt ttgtgtatag cttgtgcctg tggtaatcag gttgccatcg gttggtgcat
     5461 cgtaccattg cagtgttccc gtaccggcag cggatagcgt gagcgaggca gggccgcaat
     5521 tcgatgctcc ggtagtggtt ggcgatgcgg gcaatgccac ggtaatgtaa ttgggtttaa
     5581 tttcggtatc ggagctgtag ctgtttgaag cagtcagggt taccgtaaat gtgccgctcg
     5641 tagtgtacac atgagtgggg ttttcatctg tggaagtatt cccatcaccg aaatcccaga
     5701 ggtaggaact ggcatcaacc gaagtattgg tgaaatttac aactcctgtg caagtgctgg
     5761 tagcatcagc agtaaaaccg gcaactggaa cgatagtgaa aggggtaacg gtgatataat
     5821 tcactttggt ttcggtgtcg aaaccgttgt tgttgtttgc tgtgaggcta acagtgtaag
     5881 tacctgctgt agagtaggtg tgtaccggat tctgggtggt ggcagtctgg ctgtcgccaa
     5941 agttccacga ccagccggta gcgtattgcg acaaatcggt gaaggctaca ctctgccctt
     6001 ccataatggt ggtgggagtg ccggagaaat cagccaccgg aggattggtc gaaacaggcg
     6061 taatggtagc aatgtgggct gctctgtctt gttttgtggc aacaatgtcg attgggtttg
     6121 cattggcaat ggcagaaaat gacagggtaa cactggtacc gctacccgac cactctgcat
     6181 cccataaaac cccattctga gaaatagcca cataagtgta aggttcggtt gttacggtaa
     6241 gtgaggtaga accaaccgac aaaccaccat aactgatagt tagtgtttca ggctctgtgt
     6301 tatatggcat taaagatgga tctccaacca ggtggtatat ttcccaataa tattgtttta
     6361 ttgaagacga acttgccgaa accgcaagat tccctttgtg aactatttgg cgccccgaaa
     6421 aataccaatc agaaaaagct tctccatgat tgtgccataa tccgtcgtaa gcaccaagcc
     6481 ctgtgttagc ataggtatga cttgcccagt tggttgtgtt cagagtgagc gatgttaatc
     6541 ccattcccca ccatgcatct tcgctccagt aggaataatc ggaagcgccg atataaccta
     6601 cagccccttc gttcgcagtg taaagcagca tttcgccaaa tgcatccgac tggttaaaca
     6661 tgaacgactg gcagcagttt ccaatcataa aaggatactt gttctgatta ttgaaactag
     6721 ctatatcgga tcgtgcaatt tttgggtcag cccaaccatc gtaactgcag tgtgctgtat
     6781 agttcgaaaa accaacgccc gtactgattt cggatagaat tgcagcttcg gcacccgtat
     6841 tacttgaagc caatacgtta tatgcatgac cagcattgtt gtaaaaaaat gcccatacat
     6901 tagtaaatct gtcagaagct tcgtaatagt aattgtttat accatacaga atttgtgaat
     6961 cgccgtaaat atgtgcgctt gaagcatcaa cacccgaaat caactggcaa ttatttaaaa
     7021 aagtaccact tggaatagtg tactgttcat acggtaatat tttattcagg gcattttgca
     7081 attctgtagt actttcagca gatatacgac cgaaatacat atcaggcaga taatctgtcg
     7141 tgccatcaaa tgtggtaaaa tacacatcgg tgatatgctg attgtaggta gaactggtaa
     7201 gttttgaagc gaaagccgga atggaatagg taccgctgtg gtcaccaatg atgagaaggt
     7261 agagaggagc cgggtcggat gaattggccg catcgtaaag accttcgata taggttttaa
     7321 tggtagtatt ggttgtgcct gtagcaacat aatgttcaat tacttcgtag cccatttttg
     7381 ttttccaaag aacgaaaggt tgcaatgtgg cttggaatgc agtgttggca acaatgatgt
     7441 acttgatggg tgcattgaag ttcgaaaatg catcttttgc ctgaggtgcc tgaaaattta
     7501 taagtgcacc ataacttccg tcgaattcat gagcataata tcttgctttc atttcttcgg
     7561 ttgtagccaa gtcggcaccg ataaaattca ctgtaaaggc aagattgttg taaacaatga
     7621 tagtattttc aacggggttg taacgatagg gcctaatttc gatacgaccc attcttacac
     7681 ctctcataac accaagaacc tctgtgctaa ttaactccga tgtttcataa gcatcggtgt
     7741 tgtaatactc gttgtcaata acgaaaagct gttgttcgat gggagtgctt ttgctgtatg
     7801 atggctgggt aggtgtaatc ctctcgatgc catagtccga caggttaatt acttcttcgt
     7861 cgtaactgtt gataaccacc tgaatttcgg ctccgaaggg aacttcgata agtttgctga
     7921 taaccggaag agaggctctt cctacatttg acggagcaac tgtgccttca atttccagcg
     7981 tggaaaatga accgttttcg ttactgatat tttccagcga aagttcttta atattcactt
     8041 ctgcattgaa cgattccgca cttaccgact tcatatccag ttgtgttact gccgaagaaa
     8101 gctggatgtt ctgtttttga gcaaaagcta cagtgcaaaa aagagtagta attgcaaaca
     8161 atagaaggtt tttcatatat cctgtatttt agattttaaa atataaccga attgcacatc
     8221 tgaagaccaa aatttaggga aacaagttgc cttaaacaga gggaaagata agtaaatttt
     8281 agtttcaaat aattcttgtt gttattgaat tataaactat tagatgaaaa ttaaatttta
     8341 ttttaataag ttaacgaaac attatactca ccaccctgaa caacccagca gtcgacatca
     8401 caaattccgg taacgagctt ggcttcgagc cgtcccccgt caatcacata gattgaatcg
     8461 ttaccgttct tgtatgcggc tttcaccgaa taatacgcat tcatcggtac ttcaagtatg
     8521 agtgcactgg tgtcgcaagg agcactgaaa actatgggcg atgcatcaac ctctccggaa
     8581 aatacagtaa ttggtacttc agaattttca tcgttcagag taaattcaat aatgagatat
     8641 ccggtttcca gcgtatcggt atagcattca taacaattca catcatcacc ggtatagtat
     8701 atctgatctc tgcacgaaag cagtgtggca gtagatacga tgattatcag gatgaatagt
     8761 tttctcattc ttttataata tatctgtttg cattcactga atcggggctt tttatgccga
     8821 atgcgcccct tatcccgatg cttacccggt ggggactaat gtttttgttt ccaagatcaa
     8881 ggtaactata tccaatactc atccctgctt tggatgaaaa cctgatgtat agcccggcat
     8941 ttggactgag gctaaggaaa ggaatattct ttttatcggt ggctttatag cttccgcctg
     9001 taaaaacttc atccacgccc agtgtaagat gaagtggaaa ttcaggtctg tcaggaattc
     9061 tgaaatgctt ttttaatcct acagctattc cccatcgttt ttcccaatat tgataataca
     9121 cgccttcatc gtgttgaagc caaactctgc gggctccggg gcgtacccat gcgttgcccg
     9181 aaagttgaaa tccataacgg gtgtcgtaaa ggtcgaggcc tatgcccaca aaaacatcat
     9241 ccagattgaa agggaacaac aagctgactc caagctgctg aaagtagggt ttaaccattg
     9301 gttttattcc cttactccta accattcttt taatatctcg attgcctgaa agcatggcga
     9361 ggttcatgga attgtaataa ggtgtgatac tgtcgtttaa cacagcccca tgatcaagca
     9421 gcagttttat aagcacctga tttccgttga ccagggcttc atctatcaga gatgtgccgg
     9481 aattgctaat atcggttaca acaagtcctt catgaataag aaatgctgcc atatcggtaa
     9541 cattgtattg cacagcaata tgaacaaacg attcgccatt gctgttacgt tcagttatat
     9601 cggcgccata tttcatcaac aactgaagca ttgcagtgtc gccatttact actgcataat
     9661 atgcaggcga acatccaagg gagtcttttt cattaacagg ggcatcgtaa tacagcagca
     9721 tgtcggtcat gttgtaaaaa cccgatatta cggcatggtg caaggcttgt ctgcccaata
     9781 tgtcggtatg ttgcaatctg gcatatttcc gtattaaaaa ctcagctaca ctcatgtcgt
     9841 tattcttcac tgcgctgatt aatgctgaaa ctctttccga ctctgaatac gcatttacat
     9901 tggctccttt cccgataagg tactgaacga tttcgtagtg tcctgactgg gaagcaagca
     9961 ttagtggtgt gacattatcg gaatacgcca catctacaaa aatgcctttt tcaacacaaa
    10021 gtttcaccat ttcgaaatta ccggtgtcgg ccgccatcca gaagtcgtag gcatcagccg
    10081 caatggtatc ggttttggta tattgtgcaa agagattgct gccccaaagg cacattattg
    10141 caagaaatat aaatcgcctg atcactacag ttaaattttg aggataaaaa tagtaattct
    10201 tcgtaaaagt atatttggat atttgattat ttttgccgtg aaaatttggc tccgtagttc
    10261 aatggataga atgtcagatt ccggttctga aggttggggg ttcgagtccc ttcggagtca
    10321 cgaaagtgac gcagcctgat gcaatatcat caggttttgc atttatgcaa aaaggggcga
    10381 gaagtttatc ccgacaacca ggtcggggag tcccttcgga gtcacgaaag tgacgcagcc
    10441 tgatgcaata tcatcaggtt ttgcatttat gcaaaaaggg gcgagaagtt tatcccgaca
    10501 accaggtcgg ggagtcctta aaaacagcgt gagagtagga gtgagtacag gggaaatgaa
    10561 aagatataat caggcggagt cgggctcata catgcagtta ctcgttaatc ttatacccca
    10621 cccccttaat tgtttttata aaatcgtctc caaatttttc acggagcttt cggatatgaa
    10681 cgtcgattgt acgatcgcaa acgaatgtgt cgccccatac tttctgatat atttcgctgc
    10741 gggagaatac ttttccgggt ttcgatgcca agagttcaat taattcaaat tcttttcggg
    10801 gcagatgaaa taactttccc ccttcggtaa tttcatatct ttccctgtcg ataatcagat
    10861 tctgagtgct gatattttct acttgtccgt ttttaatagt ggaagaaact actgtttcac
    10921 gccgttttaa caaagacctt actttagtaa tcagcacttt cggattgata ggttttatga
    10981 tataatcatc agctccggcc tcaagcccgg caatttgtga gtaatcttcg cctcttgccg
    11041 taagcataat tatgagagga ttctgttcgg gtaggctatt cctgatattt tcgcacaatt
    11101 caataccatc agttcccggc atcattacat cgagtataat tgcatccgga aaaaagtttt
    11161 gcaccatcgg caatactgaa tcggaattat ttagcacttt ggttgaaaaa ccgtttttgg
    11221 taaaattata atctaaaaac tcaagaatgt cttcatcgtc gtcaaccaaa atgattttat
    11281 atattttttc ctgtttcata taatactgat aattactttt ggagtaggtg cccgaagcat
    11341 cgcaattcac cgcatcaaaa atatcctgac aatgataagt caatattaag caacacttaa
    11401 gtaattctta agctttacat aactcaggta attaactcat gctatttatt gaagttaata
    11461 gccagtttga gttggaataa tcttcccgct gagagttttg aaaaatagcg atcctctgcc
    11521 atatacgatt tgaaaactgc ctcctgtttg ctgttgagga tgttgtcaat cttaaacgaa
    11581 atctgtatat ttttctgcct gcccacattt aggtttgcat taatattcaa gctgtgaaat
    11641 ggtttcgacc atatgtcggg tctgtcggct ataccagcat aaagcagggt tctgccctgc
    11701 acattgtagt aaaccccggc ttctattccg ccgattttgt cttcaggagc tttatacgag
    11761 attcctgcat tcactatgta aggcgattgt cccgccatcg ctctgtggtt gtcaataact
    11821 tgcccgatac gggcattttc ggtgcgcgaa gcaagttcgg tggggcttag cgtaattcgc
    11881 gataaggtat aggtaatatt ggtattaacc gaaaagcact ttaaagcagg ggtaatgaat
    11941 ttcaggtttt tgcgaaattc taattctgct cctgcaactt ggccgtcacc aacattacgt
    12001 ggttggaatg cgcctacctg tgttgcaaat tgcacaattt caataggatt gataaacttt
    12061 ttataaaacg ctgagaccga caggagttgc tgctctgcgc caaacagctc ccaacgaaag
    12121 tcataattaa aaatgtcggt gctgattaaa ttcccatccc agtattcgac accggcaagg
    12181 tcgttggcat cgcggaataa tccgcctata aatgtacgcc ctgtaatggg atcgaatatt
    12241 tcggcgtatg aaagctcttt aaatgaaggc ctggcgatgg tttttgaaaa tgcagccctg
    12301 aggttctgat tatcactgat tgtaaatatt agattaaccg ccggaaagaa acccatatcg
    12361 gacaatacta catcattttt caggatatga tagcccatct ggtcccgtcc ggtatatcgc
    12421 tgtgtataat tttcggtgcg caggcccagg gtggttttca ggcgagtaag tatgctaaat
    12481 tcgagagaca catatcctgc ggcatttatg atgtttgaac tatactgatt gggattcacc
    12541 ggtataaacg gagtttcgta agtggttccc aatgtggcga tgccgttcat gggccaaatg
    12601 ttttcggtat cgaaaatctc gttggggttt cctgaaagat cgactccacg aatattgatg
    12661 gcaaaattcc ggattacaaa atcacgttgt ttatatgtaa atgctcctcc aaatcccaaa
    12721 gtggctttgt tgccaaatag cataaaccct tttgatgcat ttgcgagcgt cgaaatattg
    12781 tattcgagaa gttttctcca gattcgttcc ggaaagccgg attctgtgct gatgctatat
    12841 acgccttcgc gatcttcgta gcgggtaaaa cggacatcgg ggtcataaat caccgacaaa
    12901 gtgggtgata gcttccactc aacattccat tgagaatctc cgaaattgtg ttttccatca
    12961 ataataatat tgctaatact tcgctggcta taatccaacg tgtgtataaa accgctgaaa
    13021 ttggttccaa gattcgattt ctcataatcg aatattcccg cctttgattc gccgttttgc
    13081 aggtgcatca agttaatcct gtacttggaa tttttcgttt ttaccgctat tccgccaaga
    13141 aaatttaaaa agacagtttt aactccgaaa tcgcccagtt gatattccct tcgatcaagc
    13201 tcaaagaccg atttatcgcc cgcaaggcca taacgaccgt attcagcgtt ctcgaagtaa
    13261 tcgctcgaaa ttttatagga gaaagccgta gtgtaaccca acgtaaagtt ttttttctga
    13321 atctgattcc ctaaattggc acccatactg aaatcacaaa ggttcatctg tcggctggca
    13381 gccatggttg gattaaaagc agataatatt tctttatatc gctgcgcttt ttcactttcc
    13441 gtattgccaa caacctcagc aaagaacgga atattgctgg tggcaggaat tgcccggcta
    13501 ccatcgtcga agcccagaaa atcggtccta ccaccctgat atcccacaaa attatcaacg
    13561 aagtgtgatc cgaggttata ggccagccca aaattgaatc cggactgtct ggtttcggga
    13621 aaatctttgg ttacaatatt tattatccca ccggtaaaat cggccggtag ctcagcgcta
    13681 aatgtcttac taaccataat attatcaaca atatttgaag ggaaaatatc catttgcact
    13741 gtatttctgt cagggtctaa tccgggaatt tccaatccgt ttaaaactgt ttttgtatat
    13801 ctgtcaccca agccccttac gaacacatat ttacctccgt taaccgaaac gccggacact
    13861 cttttcattg atgaggctac atcggagtca cctgttttac gtatccctac cgatgatacg
    13921 ccgtcgagca tggttaccga tttgcgtttc atggctatca atgcattatc gttgttacga
    13981 acctcgctgg cagctattgt tacttcctga agcaggatac cttcctcttt catgagtaaa
    14041 tcgccgagtg agacaatctg tcctgatttt acctcaacct gagtaagaga aatcgtttgg
    14101 tacgatataa aagaaatctc cagttcatat tttccgggtg ccatttgtaa actgaatttc
    14161 ccgtcgagat cggtggttgt acccagggtt gtacctttta ctacaatggc tacagccggt
    14221 agcgattcac cgttttgggc atcaaaaacg cttcctctta cgaaaccatc ctgtgcagag
    14281 gctgtaataa gtgcaaaaag taaaaaaatc aatgcaacac aattcttcat catttgtagt
    14341 ttttatccgt ctttatttta acccggaaac ccacaacttt aaaagctgcg ggtttacctt
    14401 attcataatt ctgttgtttt ttagaaatca ccgagttcat tgtttgtcga aacccatgtc
    14461 catgatgcga atttggtctt atcggcacca acggtattgg cccctgcatt aactgaactg
    14521 gcatgaacat cagtcccatt tttgaaaata ctgtttacgg cgacaccagt ggccggggta
    14581 acctgtaaat tactgaatgt taaaacaccg cttgcaaaat tggctaatgt tgcatctccc
    14641 gaaagcgata agtcgccacg accatcggtt gtagccgggt cgggaaagtt aaagaaatgc
    14701 aggttttgaa aagtgcccct tgccccggaa cggaaatcgc caagttctgc tgactccgat
    14761 ccttttatac taccgttttc aagggtgtga gcggccagca ttgagccttc gggcccatca
    14821 atttcgaggg tgtgatcggt ttctgctcct gcaattacaa tgaaattatc aagtgttcca
    14881 gcccatgact ggtcggtgtc aaccccatca tcacaggggt tccagataat caggttattc
    14941 acattcacag tgccaccgaa aaattcgaca ccgtcatcct gattggcaac cacttcaacg
    15001 tattcaacag ttgttccgga gcctacccca cccagggtaa gaccgttgat ttcgttgcct
    15061 tcgccaatat ttgcaccccc atgacggata gatacatagc ggatgatacc tgaattgtcg
    15121 gtggcgttgt ttcctccgta taaaccattc tgatcggaag cgggaatccc ttcaatttgt
    15181 acagattcac cgctagccga aataggcgca tttcccagaa taattaaccc tccccaaagt
    15241 ccgttcagat cggtatctag gttcgggctg gctatcattc ccggctcaat ctcatctgca
    15301 actgaagtga atataattgg ctgtgcttct gtgccttgtg cattaattgt agcgccgcgt
    15361 gcaatgatta atgcggttgc attggaaccg gttccggcct gtcccttcac tatcacccca
    15421 ggttcaatgg tgagggttat ccctgaaact accgtgattc gaccacctaa aatgtaagta
    15481 ttaccggttt cccatattgt gttggctgta atgttaccag taatggttat ggtttcgtca
    15541 tcagggatgt cgtcttccgg atcgcatgag gttaaaacca ctgatgttgc tgcaagaatc
    15601 atcattaaat aaaacagttt tttcataact tgtagagttt aaggtttgtc ataatattca
    15661 tgctgcaaat aaataatgat aatcctatct ggattttaag ccatatctaa caaatcttta
    15721 tttatttgtt aaggaatgca cccaatgtta actaagtgta aattgaatga tataagcctc
    15781 aggatttcga agtccgcatg tgatgggata agatttcagt aaatatgttt cacactatgg
    15841 gaatgcccat agaagcgaat tttcagcaag caggtgatgg agttagattt aaggttttaa
    15901 ttgccagata ttcccattct gtttttcatc gatccggata attccggcat ttttgctaaa
    15961 aaatacgtgt tgaacattgc tttccggata attcaggttt attgcccgat atacctttgt
    16021 aaacatggaa tcggaaacga cataattacc caaataatca aattccggca tcatataaac
    16081 atatggggct tcgttttcga aatcgagttc aaactcgaag ccatacactg aataatagta
    16141 tctgtagctt tgatttgatg aaaaaattac cagcttcagc aatacaggtt tcagcccttc
    16201 gggtacgact gaaaaacggg cttcagcata gcgctcacaa ttgcatattc cattaagatc
    16261 gttacattcg taacaatagc tatcagaaac cgatatttcg ggtaccacca attttaaata
    16321 ttctgaatca gcatttacaa aacaaagggt atcgccactt ctgtaaggaa aatgttccat
    16381 atcatcaccc gaaaactcag gacaatgggt ttgattccgg catgaaacca tgaatattat
    16441 accaatgaat gcaaacaaca ggagtgtttt gttccccata tcgaaagtgt ttttgtgaaa
    16501 ataataaaaa aagcggtttg atcgcaaacc gcttttaaaa ttttaatatc agggttattt
    16561 ctggccaccg caacctgacg ctttcccttc gccggaatta tgctgaccag cgcaacccgc
    16621 cggttttgct tcgttgcttg ttttgcttcc cgggcaaccg ctcccggcat caccacttga
    16681 ggcttttttg ttaggacagc cgctatgatt cccggggcac tgtttggcat ttgcatcata
    16741 tacaacggcg tcgtattcta tttctttaag cgatgcaatc agtttgtttg gatcagtttt
    16801 ggtgctgtta taaaccacta ttaccacatc tttttcaaag tcggctttca ccgcccgcac
    16861 accttttgta aaagcaagtt gtttttccac ttttgtggcg catgcctgac agttcatgtt
    16921 tactttgagt gtaacttcct tatcgttacc tcctttggtg ggcatggcgt ttacactttg
    16981 taaagcaatc atcgaaatga atgcaaatcc gataaataat gctagttttt tcatattcat
    17041 tttttggttt tttagttatt aatttaaata caagtttaat tattaatttg gttttgtaat
    17101 atccctctca atagcatatc gtatgccaat ataaaatttc cgcccgtgaa ccggt
//
LOCUS       DAOOQP010000060        16960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4117_length_16960_cov_5.750665, whole genome shotgun sequence.
ACCESSION   DAOOQP010000060 DAOOQP010000000
VERSION     DAOOQP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4117_length_16960_cov_5.750665"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLK75_07540"
     CDS             complement(<1..346)
                     /locus_tag="PLK75_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOY39138.1"
                     /translation="MGLFDKIKAEFIDIIEWLDPSNDIIVHRFERYHNEIKMGAKLTV
                     RESQVAVFINEGQIADVYTPGMYTLTTENMPILSTLKGWKYGFNSPFKAEVYFVNTKK
                     FLDNKWGTPNPVM"
     gene            complement(350..709)
                     /locus_tag="PLK75_07545"
     CDS             complement(350..709)
                     /locus_tag="PLK75_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39139.1"
                     /translation="MDSMEYIDYEAFEKRTMNDVGFQQEFLGLSATLFEQYHDQMSEA
                     AEKKEYVIIKGLAHKAKTGAALMGMDNVSAEYQKLENEILVDHYDPMMIVRIEAIRNA
                     FNNCIVLLKQYINSKNK"
     gene            complement(848..1558)
                     /locus_tag="PLK75_07550"
     CDS             complement(848..1558)
                     /locus_tag="PLK75_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY39140.1"
                     /translation="MKCIIIDDEPLAREGMKMNISEVQSLELVGEFDDAIKATDFLHE
                     NDVDLMFLDIEMPGVSGLNFLKDLTNPPLVILATAYPQYAVEAYELDVVDYLVKPIKL
                     DRFLKAIAKVEEVMKLTKNTSMLEAIEDNFIFIKSDRKYVKINFEEILFIEGLKDYVI
                     LHTLHGKYMTAMNVKTIHNKLPEDIFIRTSKSYIININHIKEIDGNDVIIHDNKIPIG
                     RSYRDEFLEFVNKRLLKR"
     gene            complement(2379..4100)
                     /locus_tag="PLK75_07555"
     CDS             complement(2379..4100)
                     /locus_tag="PLK75_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39141.1"
                     /translation="MHVPAKIAKTCYLLIIILSLTPSVNLFAQTQEVKLKVYQYNINK
                     DIVEAVFRSFPDSISAIQYLNSFRTDYLSKGYLAAGYDSIVFRKNDISAYFLKGLKYS
                     WENIQVRAEDTITAKIYNLRRYKDDKITEKEFGYLLNDVVSAYENIGYPFCVVWLDSI
                     SFRNNLVSAVICTDPGDFIVLDSLILKGNSRVKLPYLKKYLGFKDGSIYKEKYIESMN
                     TSLNNLAFINQSKKPEIAFGKNTADVILYLSDNKTSSFSGIIGFLPEEQTSGKLLVTG
                     DADLFLQNLLGRGEHLALNWTRYQALSQTLKADFSFPYFLNSNIGIAAGFDMFKQDTS
                     YLNLTSRVGLRYMAQANNGVLVFYENISSFPIGNTEESGSMANYSGFTSHMLGLGYSV
                     TRYDYFYNPSKGYSLSGEASSGIKKTDSDTSIKSNPVVMLGINAKLYIPITQRIVWKQ
                     SIASKALFCSNIYRNELFQLGGIKSFRGVAENTLEASSFAIVTSEIRYLFERNSAVFA
                     FCDYGCIYNEVTTVDRLGFPVGFGVGIDLQTKAGIFSLAYAYGIFGQGSFDLRAAKIH
                     FGYIARF"
     gene            complement(4103..5266)
                     /locus_tag="PLK75_07560"
     CDS             complement(4103..5266)
                     /locus_tag="PLK75_07560"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOY39142.1"
                     /translation="MRKLTILLFVVGLAVASCKTNFNVVHIKDSNYQIKKSGFFYSLP
                     RLMFEVKLQIEKTQFVAGPYCNYANKYLGIENVVTENYTEYQISSVLLTPLYEPDPAA
                     VFFVDPGSKNGGCKLAISNNGILTGYNIEEPDDPEVQTEINPIIVPSHSGKTVWFTDL
                     TVKRNFSDIQDTTYRVIKTDSVYQKIPIINTKITSKDIEQKAEEAANFVIKLRKRRFK
                     LETAQTEQIPDGYAMEKMIGSLNELEKEYIDLFIGKKHTQEEVYCLFFIPEKNTTPVI
                     LFWFSENEGVSTSEKEGFYPVTVEITNYEINDEISRFYNRQTEFSPKYRGLYYRIPGQ
                     ANMKIFADGVCYLNDVFTVPQFGHLNFIDEQLMKNKNLQISFDKNSGALRSIK"
     gene            complement(5273..6781)
                     /locus_tag="PLK75_07565"
     CDS             complement(5273..6781)
                     /locus_tag="PLK75_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOY39143.1"
                     /translation="MKVFTAAQIREIDKATIQLEPISSVDLMERAAGEIFFLLEVFIT
                     KLHKVVVICGQGNNGGDGFVLGRMINNSGFNCMVYVVQSERGWSEDALTNYERYRKLF
                     PEKIKTIGNITDLPTLDISTIVVDAIFGSGLNRPVEGPEAEVIKFVNDTEAIVISVDI
                     PSGLFAEDNSDNTGAIMQADFTITIQTPYLSFFFPENARFVGEWYIAPIGLHKETIFN
                     TPTPYIYVDDELIAEVFRTRKKFAYKNNFGHALLVAGSKDKPGAAVLAAKACMRSGCG
                     LLTMHVPKGVQACMVEAVPEAMLQVDENDNDFTMITGINSYDAVGVGPGLGTGEAVRS
                     AFRLLLTENKHPLVLDADALNMISQEKDLLNILPENTILTPHIGEFKRLFGDYENSYL
                     RLQAQMDLSVRYRIIIVCKGAYTAVTTPDGHCYFNSTGNQGMATAGSGDVLTGIILSL
                     LSQGYDAAMAAIFGVWLHGRAGDLALDKQSYESLIAGDIINNIGGAFKTINK"
     gene            6904..8493
                     /locus_tag="PLK75_07570"
     CDS             6904..8493
                     /locus_tag="PLK75_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOY39144.1"
                     /translation="MFKNHPKGLLAASLTNMGERFGFYTMMAILVLFLQAKFGLNGKD
                     AGLIYSVFYFSIYILAFVGGLIADRLRNYKGVILTGLIVMAIGYLMIAIPTKTPVDNQ
                     TLMLILTCSGLFVIAFGNGLFKGNLQALVGQMYDNPTYGKMRDSGFSLFYMFINLGAI
                     FAPFTAIGIRNWWLGKHAFEYNANLPALCHQHIEGTLETTKIVGSGALDKAQLLAEQT
                     FALDPTSKFQSLATEVSSAATNLTSFANEYLNVFTTGFHYAFVAAICAMGVSLLIYLL
                     NRKTFPNVSKKAAGKNETGDIKMEAKEVKQRLYALLAVFAIVIFFWFSFHQNGLTLTM
                     FARDFTDLSGIKVNLGFVTIQGAEIFQSINPIFIVLLTPIVMAVFGWLRARGKEPSTP
                     KKIAIGMGIAALAYVVMTIGSYGLPLVKDIQGSPLDDAAKITPMLLIGTYFILTVAEL
                     FISPLGISFVSKVAPPHLQGVMQGGWLGATAIGNQLLFIGAVLYESTPVWLTWTVFVG
                     ACFLSMLTMLLMLKWLERITK"
     gene            8530..9000
                     /locus_tag="PLK75_07575"
     CDS             8530..9000
                     /locus_tag="PLK75_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39145.1"
                     /translation="MKKILYLSIAVVLTLSIASCKKYDVAKDVDMSILKLNQFNDKFA
                     EFYKDEVISKDTLENEKSSEFDQLKKIAGEYYEIINKINSQIKEDQERVKNGKKSKGY
                     EDDYKKALSEKNDAIEKSTGLFIENMNKLNGTDEINLDEVVPEQAEQVETLLVK"
     gene            9093..9728
                     /locus_tag="PLK75_07580"
     CDS             9093..9728
                     /locus_tag="PLK75_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOY39146.1"
                     /translation="MNTENNQSTKTKGLGCLGALLIGTPILVILLLLTIWGIRIYNKT
                     VERDETVKKQWSQVETAYQYRYDLIGNLVNVVKGYADFEKSTLTAVIEARSKASSIEI
                     KAEDVNEATLQELDAANKNLNSSLDRLMVVVEQYPNLKADQQFMSLSAELKNTEIQIK
                     LARDAYINVVKDFNAYIRKFPRNLFAKLFGFNQYKYYESDEGAEDKIDVNL"
     gene            9880..11328
                     /locus_tag="PLK75_07585"
     CDS             9880..11328
                     /locus_tag="PLK75_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOY39147.1"
                     /translation="MSPVLSGMKTCAKIFLSNTAILLSVGLHSQTLTTSEYFDLVSRN
                     VSQSDNKVLKCNCIGISFDSRLNEFPGAIMIAGETYAISDDVKYAYNHLCELPDKDKC
                     KILSQYYFLTKLISPLSNSGVISSDFACLPIALTGMNHLFAGADETAGIWKLQYFPAL
                     RYGLFADSCRDDRFDISLSTKAAVTYLNTLHELFGRWDLAILAYTSSPAYVNKIIDEN
                     SGFMHENDKAVFNVFIASILWCNSHLNQVQKIHYGFLPEVLDSVTVQKKIHTGQLEAV
                     LHISTDSIKALNPHIICDVIYGDLKPVLLILPSFYAKLFVEKQDSIAIFRDSVFFPKP
                     KIPDGNYATHKTPEYNSSDYEKIEYIIESGDNLGYIAEKFHVSINDIKDWNDISGTTI
                     YAGKKIIIFKKKTVAKTNTPTAPKQNNTPKTPDMKNLVFQENYVVQKGDYLYEIAKKY
                     PGVSAEDIMQWNNITNPEKIQIGQTLKIYKKK"
     gene            11325..12776
                     /locus_tag="PLK75_07590"
     CDS             11325..12776
                     /locus_tag="PLK75_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39148.1"
                     /translation="MKPRQVLLFVAVVIGMLGIIMAIFPRSGIRLTNNLVLYFPSFSE
                     MFSKKDARLDIDSLLSKQFNIDSLGMKVDDSLSPLDIEELKKLITPLEFPQNNPHALD
                     PFFAKLNNPDEDGRIRVMHYGDSQIEGDRITAFLRNKLQSRFGGLGMGLSTAGVVYSQ
                     FSIIQENSSNWTRYPGFVMINPLVKHKKYGAMIAFSRFAPIVDSGATLPETKYKAWLS
                     FTKSDLAYSNTKKFKNVSIYYGNAKARVKISIISGETVLVTDSLKAGEGMTVFEYKSS
                     TYLENIRFEFEGNDSPDFYAISFEDDKGVYIDNIALRGSSGTVFTTTDGSLLSQMFTR
                     LDVDLFILQFGGNVMPYIKDAKNAALHASYFYSQLLFLKRLVPDACIIVIGPSDMSYK
                     EKDYYTTYPILETVRNELKAATLKAGGVYWDMYEAMGGKNSMISWVNANPPLAGADYT
                     HFTPQGTVVVANMFYNSLILEYSEYNERIKAPK"
     gene            12773..14029
                     /locus_tag="PLK75_07595"
     CDS             12773..14029
                     /locus_tag="PLK75_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOY39149.1"
                     /translation="MKNIILSFVICTSVIYGASGQTSRIPYRNYTFGYIESDSVLFRT
                     YGDSSTINCFYEKLDRLIFEGSGNLHIVHFGASHIQAGIWPWQIRCNFENMSRCIEGG
                     PGYVFPFSIAKTNHPYYYRSSYEGNWEIAKITDQNVSCPIGVGGITAICSDSIAEIGI
                     QFNKTADIYKHSFDKVSVFHSDIKGTYSVSLIPESAVNYSTFDSVSGATVFYLNRECD
                     SLNLLFQKTDSGDDTLYFYGAYLVKMTNGIHYSGIGINGASTISYFKAGRLQQQLCCI
                     DPDLIVFSIGVNDASGKNFTGDRYADNYWVLLSMVREVCPAAAIILTTNSDFYYYRGS
                     HNEHAGEVLQAMNQVAAEFGASVWDFHAAMGGNRSINLWKQDGLAQRDRIHFTQEGYT
                     ILARLFFEAIQKDFEQYIIRNALNQQ"
     gene            14031..15692
                     /locus_tag="PLK75_07600"
     CDS             14031..15692
                     /locus_tag="PLK75_07600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOY39150.1"
                     /translation="MSISNIWDIFVYDQNAPLIFTRFFFWGFFAVVLLGYSFLAKKNT
                     ARSLYLLIFSLFFYYKSSGFFFFLLIFSTVVDYYIGKWVYKSNDNKKRKWLLALSIVV
                     NLGVLAYFKYAYFIVEAANQMFGTHFTAYNYLAEFSNLLTGSHFDTSKIFLPVGISFF
                     TFQTISYTVDIFRRKLAPVKNIIDFGFYVSFFPQLVAGPIVRASEFVPQIYEKFRLNR
                     YEFGMALFLILKGLIKKIMIGDYIAVNFVDRIFANPDMYSGLESALAMIGYSLQVYVD
                     FSGYTDIAIGVALLMGFRLPQNFNSPYKAKNCSDFWSRWHMSLSSWLKDYLYIPLGGN
                     RSASPGTYINFALIIAVFVLLAANTWVTIILIAFTIVVLLLMRFSVKARKQIQTNLNL
                     MITMLLGGLWHGSSWNFMIWGGLNGLGVLVYKYWRKISPWEHTNHWIATVWKIALTFT
                     FITFTRVFFRAPDYTTAETMLKRIWYSFNGSVFFQVMAAYYKVWLIMLFGFVTHWLSY
                     KFKDKWRDRFIGMPHWAQAVVCTLVVFVVYQSICSDMQAFIYFQF"
     gene            15868..>16960
                     /locus_tag="PLK75_07605"
     CDS             15868..>16960
                     /locus_tag="PLK75_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOY39151.1"
                     /translation="MSKIKRVYTFGNKNAEGKSDMKNLLGGKGANLAEMCLLGLPVPA
                     GLTITTETCTEYYENNCQLPDGLMEDVWKGMELMEKNMGMKYGDKENPLLVSCRSGAR
                     KSMPGMMDTVLNIGLSSATLPGLIKKTNNPRFVYDSYRRLIMMYADVVMDKAEGLDKN
                     IRRQLDEMLDEYKHAKGYKSDTDLSTDDLIHLSEAFKKKVKEVLGTEFPDDAKEQLIG
                     GIKAVFKSWNGKKAISYRRIESIPDNWGTAVNVQAMVFGNMGDSSATGVAFTRNPATG
                     ENLFYGEWLINAQGEDVVAGIRTPNPLNSDTKNEQNKHLHSMQELYPVIYKELCDIRE
                     TLETTFHDMLDIEFTIQEGKLYMLQCRVGK"
BASE COUNT         5185 a         3355 c         3224 g         5196 t
ORIGIN      
        1 gcattacagg gttgggtgtt ccccacttgt tgtcgaggaa tttttttgta ttaacaaaat
       61 acacctctgc tttgaacgga ctgttaaacc catatttcca acctttgagc gttgataaaa
      121 tcggcatgtt ttccgttgta agcgtgtaca ttccgggggt gtaaacatcg gctatctgac
      181 cttcgtttat aaaaacagca acctgcgatt cgcgtactgt aagctttgca cccattttaa
      241 tttcgttgtg atacctctcg aagcgatgta caataatatc attcgacggg tcgagccact
      301 caatgatatc aataaattca gctttaattt tgtcaaaaag tcccatagtt tatttatttt
      361 tagaattaat atattgtttt aaaagcacga tacaattgtt gaatgcgttg cgaattgctt
      421 caattcttac tatcatcatg gggtcgtaat ggtcaaccag aatttcattc tccagtttct
      481 gatattcggc acttacatta tccatgccca ttaatgctgc tccggttttt gccttgtgcg
      541 ccaaaccttt aataatcaca tattcttttt tttcagcagc ttcagacatt tgatcatgat
      601 attgttcgaa aagcgtagcc gaaaggccca agaactcctg ttggaagcca acatcgttca
      661 ttgtcctttt ttcgaaagct tcataatcga tatattccat actatccatg gggtcaggat
      721 aaaattaagc aatgttcaaa tataacaata attatgattg catcgtatat tttacaccaa
      781 ctttataaca acaaaaaaag gaatacgctg cactgagcat attccttcga aatatcattg
      841 ataatatcta tctcttaagc aaacgtttgt tcacgaattc gaggaattca tcacgatacg
      901 atcttccaat tggaatttta ttatcgtgta ttatcacatc atttccatcg atttctttaa
      961 tatgattgat gttgataata taggatttac tggttctgat aaatatgtcc tcgggcaatt
     1021 tattatgaat ggttttcaca ttcattgcag tcatatattt cccgtgtaat gtatgcagaa
     1081 taacgtaatc tttcaaacct tcgatgaaaa gaatttcttc aaagttgatt ttaacgtatt
     1141 ttctatcgga cttaataaaa atgaaattat cttcaatggc ttccagcatc gaagtgtttt
     1201 tggtcagttt cataacttct tcaactttag cgattgcttt cagaaagcgg tcgagtttaa
     1261 tgggcttaac gagatagtcg acaacatcaa gttcgtaagc ttcaacagcg tattgaggat
     1321 aggctgttgc gagaataaca agcggaggat ttgtaaggtc tttcagaaaa ttcaatcctg
     1381 aaactcccgg catttcaata tcgagaaaca taaggtccac atcattttca tgcaaaaaat
     1441 cggttgcttt tattgcgtca tcaaattcgc caaccaattc aagactttgc acttcgctga
     1501 tgttcatttt cataccctct cttgcaaggg gctcatcatc aattattatg catttcataa
     1561 tattagtttt atttaattta acaaaatatt ttagttcatc agttcaaaat caaaaaagca
     1621 tttggcaatt ctaagcaaat tcgcatcaac agaaatacac aatcctcaaa ataataatcc
     1681 ttagcgatta ctaaacaata atcagaagga atttgttcaa aatttcaatc aggttgcgaa
     1741 aataagtatt tttattcaaa atatcaacaa attgaaagct gcgttttcaa cacgaataaa
     1801 tttttgacaa acttttatga ttttatattt tggcagtacc cgctatgcaa cttactaaat
     1861 gagcgcttca gcagtttttg atttataagt gatgttatct tacgttttca atttgaggtt
     1921 attgttaata tttatgagta tatttcattg caagcgttac aatgcctttt tgcctcatcg
     1981 caaaagttca acggaagcct gaataagaac aactacattc caccattgtt acattcggaa
     2041 aaaaacattg gtaataatcg tttttataat gaatgaaata ttacatatcc tttgtcaata
     2101 ctgatattga agtttatcat ccaatcgatg gttcagaaag tattccgtaa atatttctat
     2161 tttctgttca agcctcgaag ccgtattcac ccgttcctag tatctacaat gttgcctttt
     2221 ttattcttgc acaagcattt caagggagtt agaaaaatcg ctgttaataa gtgtaataaa
     2281 ttttcttcaa aaacccggtt tattaacaag ctttgttaac aatttactta tagcaccttc
     2341 aattatcgaa aagtgctttc cccatccgaa aattcatctt aaaatctggc tatataacca
     2401 aaatgtattt tggccgcacg taagtcgaag ctcccctgcc caaatatacc ataggcataa
     2461 gccaaagaaa atataccagc tttagtctgc aggtcgattc ccacaccaaa gcctacagga
     2521 aatcccaggc gatcaacagt agtgacttcg ttataaatgc aaccgtaatc gcaaaaagca
     2581 aacactgctg aatttcgctc aaacaaataa cggatttccg aagtaacaat ggcaaatgat
     2641 gatgcctcca gtgtgttttc ggcaaccccc ctgaatgatt ttatcccgcc caactgaaaa
     2701 agctcattgc ggtaaatgtt gctgcaaaac aatgccttcg atgcaatcga ctgcttccag
     2761 actatccttt gagtaattgg aatataaagc ttcgcattaa tccccagcat caccacagga
     2821 ttgcttttta tagaagtgtc tgaatcggtt tttttaatac cggatgatgc ctcccccgac
     2881 agagaatacc cttttgatgg attataaaaa taatcatagc gtgttacaga ataacccaga
     2941 cccagcatgt gtgaggtaaa gcctgaatag ttggccatgc ttcccgattc ttcggtattt
     3001 ccaattggga aggatgaaat gttttcgtaa aaaaccaaaa ccccgttatt tgcctgagcc
     3061 atgtaacgta aaccaactct ggaagtcaga tttaagtagc tggtgtcttg cttaaacata
     3121 tcgaatcctg cagctatacc aatattagag tttaaaaaat aaggaaagct aaaatcagct
     3181 ttaagtgtct ggctcaatgc ctgataccta gtccagttca atgcaagatg ctctcctctt
     3241 ccaagcaaat tttgcaaaaa caaatcagcg tcccctgtaa ccaagagttt tccagaagtc
     3301 tgttcttcgg gcagaaaccc aataatacca ctaaaagaag atgttttatt atcggagaga
     3361 tataaaatta cgtcagcagt attttttcca aatgcaattt caggtttttt gctttgattt
     3421 ataaaagcca gattattgag cgatgtattc atactttcga tgtacttctc cttataaata
     3481 cttccatcct taaacccaag atatttcttc aggtatggca gtttaaccct actgttgccc
     3541 ttcagaataa ggctatccaa tacaataaaa tcaccggggt cggtacatat tacggcagac
     3601 acaaggttat tccggaacga aatactgtcg agccagacca cacaaaacgg atatcctata
     3661 ttttcgtatg ccgacacaac atcgttcaat aaatatccaa attctttttc tgtaatctta
     3721 tcgtctttat acctgcgcaa attataaatc ttagcagtaa ttgtgtcttc agcacgaacc
     3781 tgaatatttt cccatgaata tttcaaccct tttaaaaagt aggcagaaat atcattcttc
     3841 ctaaatacaa tactatcgta acctgctgcc agatacccct tcgaaagata atctgtgcgg
     3901 aatgagttaa gatattgtat ggcagaaatg ctatcaggaa aagatcggaa aacagcttca
     3961 actatatctt tgttgatgtt gtactgataa actttcagct ttacttcctg tgtctgtgca
     4021 aacaaattta cagatggagt aagtgataaa ataattatca gtaggtaaca tgttttagct
     4081 attttggcag gaacatgcat ttctatttta tggagcgtaa tgctcctgag tttttatcga
     4141 aactgatttg caagttttta tttttcatca attgctcgtc gataaaattc aggtgcccaa
     4201 attggggaac ggtgaacaca tcgtttaaat agcaaactcc atcggcaaat atcttcatat
     4261 ttgcctgccc cggtatcctg taatacagcc ccctgtattt tgggctaaac tctgtttgcc
     4321 tattgtagaa acgactgatt tcatcgttga tttcataatt tgttatttcg acggtaaccg
     4381 gataaaagcc ttctttttcg gaagtagata caccttcgtt ctccgaaaac cagaagagga
     4441 ttacgggtgt tgtgtttttt tccggaataa aaaacaagca gtaaacttct tcctgcgtat
     4501 gctttttccc aataaataaa tcaatatatt ccttttcaag ttcatttaag ctgccaatca
     4561 ttttctccat agcataccca tcgggaattt gctcggtttg tgcagtttcg agcttaaaac
     4621 gccgtttccg cagctttata acaaaattgg cggcttcttc ggctttttgt tcaatgtctt
     4681 tgctggtgat ttttgtattg ataatcggta ttttctggta cacagaatca gtttttatca
     4741 cgcggtaggt cgtgtcctga atatcggaga agttacgttt cacggtaagg tcggtaaacc
     4801 atacggtttt accggaatgt gagggaacaa taattgggtt gatttcagtt tgtacttcag
     4861 ggtcatcagg ttcttcgata ttatagccgg tcaatatacc attgttgctg atagccagtt
     4921 tacatcctcc gtttttactc cccggatcaa caaaaaaaac tgccgccgga tctggctcat
     4981 aaagtggagt cagtaatact gagcttatct gatattcggt ataattttct gtaactacgt
     5041 tctcgattcc aaggtattta ttcgcataat tacaatatgg cccggcaaca aactgagttt
     5101 tttcaatctg aagttttacc tcaaacatta aacgcggtaa ggagtagaaa aaaccgcttt
     5161 tctttatctg gtaattgctg tccttaatgt gaacaacatt aaaattcgtt ttacatgatg
     5221 caacagccaa tccaacaaca aaaagcagaa ttgttaattt tctcataatc aattatttat
     5281 ttatcgtttt aaacgcaccc ccaatattgt ttattatatc gcctgcaatc aacgactcgt
     5341 atgattgttt atccaatgcc aaatcaccag cccggccatg tagccatact ccaaaaattg
     5401 cagccattgc ggcatcgtat ccctgtgaaa gcaacgacaa gattatccct gtcaaaacat
     5461 caccagatcc cgctgtagcc ataccctgat ttccggttga gttaaaataa caatggccat
     5521 cgggtgtagt tactgctgtg taagcccctt tgcaaactat aattattcgg tatctgaccg
     5581 acaagtccat ttgggcttgc aacctcaaat atgaattttc ataatcccca aacagtcgtt
     5641 taaattcacc aatgtgagga gtgagaatgg tattttcggg taaaatgttc agcaaatctt
     5701 tctcttgtga aatcatgttc aacgcatcgg catcgagcac cagcgggtgc ttattctcag
     5761 tcagtaacaa tctgaatgca ctcctcactg cttctcctgt accaagtccc ggccctactc
     5821 ccacagcatc gtacgaatta atccctgtaa tcattgtaaa atcattatcg ttttcgtcaa
     5881 cctgaagcat ggcttcggga acagcttcaa ccatacaagc ctgaacacct ttgggaacgt
     5941 gcatagtcag tagcccacaa ccagacctca tgcaggcttt tgccgccaac actgccgcac
     6001 cgggtttatc cttagatccg gcaaccagca aagcatgtcc aaaattgttt ttatatgcaa
     6061 atttcttcct tgttctgaat acctccgcaa tcaactcatc gtcaacgtaa atatatggag
     6121 taggagtgtt aaagatcgtt tccttatgca agccaatggg tgcgatgtac cattcgccga
     6181 caaaccgggc gttttcaggg aagaaaaacg atagataagg tgtttgtatg gtaattgtaa
     6241 aatcagcctg cataattgct ccggtattgt cggaattatc ttcggcaaat aaaccggagg
     6301 ggatgtctac cgaaattact attgcttcgg tatcgttgac aaacttaatc acttcagctt
     6361 cgggaccttc aaccggacga ttgaggcctg atccaaagat ggcatcaaca acaatagtag
     6421 aaatatcaag cgtcggcaaa tcggtaatat tgccaattgt tttaatcttc tcaggaaaca
     6481 gtttccggta tcgttcatag ttggtaaggg catcttctga ccatcctctt tccgattgaa
     6541 ctacatatac catacagtta aaaccactgt tgttaatcat tcttcccaac acaaatccgt
     6601 cgccaccatt gttaccctga ccacagataa caaccacttt gtgtaattta gtaataaaca
     6661 cctcgagcaa gaaaaatatc tctcccgccg ctctttccat taagtcgaca gacgatatcg
     6721 gctcaagttg tattgtggct ttatcgattt cgcgaatctg tgctgcggta aaaactttca
     6781 tcaatcctgt tttcaacaaa aatacaccgt aatgataaaa aataaaactt ggacactaaa
     6841 aaaagattta tatatttgtc tgcgctgaaa aaaaaacgag tattaactaa aacttaatta
     6901 attatgttta agaatcatcc gaaaggatta ttggctgcat ctttaacaaa catgggagaa
     6961 cgctttgggt tttatactat gatggcgatt cttgtgttgt ttcttcaggc aaaatttggt
     7021 ctgaatggta aagatgccgg gcttatttat tccgtgtttt atttttcaat ctatattctg
     7081 gcatttgtcg gaggtcttat agccgacaga ttgagaaatt acaagggagt aattcttaca
     7141 ggcctgattg tgatggcaat aggttattta atgatagcca taccgacaaa aactcctgtt
     7201 gacaaccaaa cactcatgct aattttaaca tgttcaggtt tattcgtcat agcttttggg
     7261 aatggcctgt tcaaaggcaa tcttcaggca ttggttgggc agatgtatga taatcccact
     7321 tacggtaaaa tgagagattc cgggttttca ttattctata tgtttatcaa cctcggtgct
     7381 atttttgctc catttactgc aatcggcatt aggaactggt ggcttgggaa acatgctttt
     7441 gaatacaatg ccaatcttcc tgccttgtgt catcaacaca ttgaggggac attggaaaca
     7501 acaaaaattg ttggttccgg agctttagat aaagcccagt tactggctga acaaactttt
     7561 gctcttgacc caacaagcaa atttcaatca ctcgcaactg aagtatcttc agcagcaacc
     7621 aacctgacta gtttcgccaa cgagtacctg aatgttttta ctacaggttt ccattacgct
     7681 ttcgttgctg caatttgtgc aatgggtgtg tctttattga tttatttgtt gaatagaaag
     7741 acgtttccaa atgttagtaa aaaagctgca gggaaaaacg aaaccggtga cattaaaatg
     7801 gaggcaaaag aagttaaaca gcgtttgtat gctctgctgg ctgtttttgc aattgttatt
     7861 ttcttttggt tttcgttcca tcaaaacgga ttgacattaa ccatgtttgc ccgcgatttt
     7921 actgatcttt caggcattaa agtgaatctg gggtttgtta caattcaagg tgccgagatt
     7981 ttccaatcga taaatccaat ttttattgtt ctgcttactc ctatagtgat ggcagttttt
     8041 ggatggttgc gagccagagg gaaggaaccc tcaactccca aaaagattgc aatagggatg
     8101 gggattgcag cacttgctta tgttgttatg acaataggat cttacggatt gccacttgtt
     8161 aaagacattc aggggagccc acttgatgat gcagcaaaaa ttactcctat gttgctaata
     8221 ggcacctatt ttattcttac tgttgcagaa ttgttcatta gcccacttgg aatttctttt
     8281 gtatcaaaag ttgctccgcc tcatcttcag ggtgtaatgc aaggaggatg gcttggtgct
     8341 actgcaattg ggaatcagtt actttttatc ggtgccgttt tatatgaaag cactccggtt
     8401 tggttaacat ggactgtttt tgttggagca tgcttccttt cgatgcttac aatgttgctc
     8461 atgttgaaat ggttagagag aataaccaag taattcatat taatatttaa attttaaaaa
     8521 atgaaaaaaa tgaaaaaaat attatatctg tcgattgctg tggtactaac attatcaata
     8581 gcatcctgca agaaatatga cgttgccaaa gacgttgata tgagtattct caaacttaat
     8641 cagttcaatg ataaattcgc agaattctat aaagacgaag tgatttcgaa agacactttg
     8701 gaaaatgaaa aaagctcgga gtttgaccag ttgaagaaaa ttgccggaga atattatgag
     8761 attattaata aaatcaactc tcagattaaa gaagatcagg aaagagtgaa aaatggcaaa
     8821 aaatcgaaag ggtacgaaga tgattacaaa aaagcccttt cggagaagaa tgatgccatc
     8881 gaaaagtcaa caggcctttt catagagaac atgaacaaat tgaacggaac tgatgaaatc
     8941 aaccttgatg aagttgttcc cgaacaagca gaacaggttg agacgctctt agtaaaatag
     9001 caaagtttaa atacaagtaa ttttaaaaaa gcaggctcac gcttgctttt ttttatgaat
     9061 taatcgtatt tttacaaaaa caaataacca atatgaatac tgaaaacaat caatcaacaa
     9121 aaacaaaagg gcttggatgt cttggcgccc ttcttatcgg aactcctatt cttgtcattt
     9181 tattgctcct taccatttgg ggtatacgca tttacaacaa aacggttgaa agagatgaaa
     9241 ctgtgaaaaa acaatggtca caggttgaaa cagcctatca gtacaggtat gacctgattg
     9301 gcaatcttgt taatgttgtg aagggatatg ctgacttcga aaaaagcaca cttacagcag
     9361 taatcgaagc ccgttcgaaa gcatcaagta tagaaataaa agcggaagat gttaatgaag
     9421 caaccttgca agaacttgat gctgcaaaca agaatttaaa ttcttcttta gataggctaa
     9481 tggttgttgt agaacaatat ccaaatctga aagccgacca gcaattcatg tcacttagtg
     9541 cggaactcaa aaacactgag attcagatta aacttgcaag agatgcttat attaatgttg
     9601 tgaaagattt caacgcctat atacgtaagt tccctcgcaa cttatttgcc aagttgttcg
     9661 gcttcaacca atacaaatat tatgaatcgg atgaaggtgc tgaagataaa attgatgtta
     9721 atttataaat aatcgtttcg ttttaagccc attcaccgta aggcatcacc ttatttgaat
     9781 agctttttct gattacttct tgtcaaggtt cttctatcaa ttttacatgg cttaagtttt
     9841 tctataattt cttttttagc ggaattaaaa cctatatatt tgtcgcctgt tttatcagga
     9901 atgaaaactt gcgcaaagat atttctttca aacacagcaa tcttattatc agtaggtttg
     9961 cattctcaaa cgcttaccac ttctgaatac ttcgacttgg tcagccgtaa cgtgagccaa
    10021 agcgataata aggtattgaa atgcaactgc ataggaatat ctttcgattc acggttgaat
    10081 gaattccccg gggccataat gattgccggc gaaacctatg cgataagtga cgatgtaaaa
    10141 tatgcttaca accacttgtg tgaactcccc gacaaggaca aatgtaagat actctctcag
    10201 tattatttcc tgaccaaact aataagtccc ctgagtaatt ccggagtaat aagcagcgac
    10261 tttgcctgtc ttcccattgc cttgaccggt atgaaccact tgtttgccgg agctgatgaa
    10321 acagccggca tctggaaact tcaatatttt cctgcattgc gatatgggct gtttgccgac
    10381 tcatgccgcg acgacaggtt tgatatttcg ttgagcacga aagcagctgt aacatatcta
    10441 aacaccttac atgaattgtt tgggagatgg gatcttgcta ttttggcata tacctcaagt
    10501 ccggcgtatg taaataaaat aatcgatgag aattccggct ttatgcacga aaacgacaaa
    10561 gcagtgttca atgtttttat cgcttctatt ttatggtgca acagtcattt aaatcaggta
    10621 caaaaaattc actatggatt tcttcctgag gttctcgatt cagtaacagt tcagaaaaaa
    10681 attcataccg gtcagttaga agcagtatta catatcagta ccgacagtat aaaagcttta
    10741 aacccgcaca ttatttgcga tgttatttat ggtgacctga aacctgtact tttaatttta
    10801 ccctcgtttt atgcaaagct gtttgttgaa aagcaagatt ccattgccat tttcagggat
    10861 agtgttttct ttccaaagcc taagattccg gatggtaatt atgctacaca caaaactcca
    10921 gagtataaca gcagcgatta cgaaaaaatt gagtatatta ttgaatccgg cgacaattta
    10981 ggatatattg ctgagaaatt tcatgtgagc attaacgata taaaagactg gaacgatatt
    11041 tcgggaacca caatttatgc aggtaagaag attattatct tcaaaaagaa gacagttgcc
    11101 aaaaccaaca ctcctactgc tcccaaacag aacaatacac ccaaaacacc ggacatgaag
    11161 aatcttgttt ttcaggaaaa ttatgttgtt caaaaagggg attatctcta cgaaatcgca
    11221 aagaagtatc cgggagtctc ggctgaagac attatgcaat ggaataatat tactaacccc
    11281 gaaaaaatcc aaattggtca aacacttaaa atttataaaa agaaatgaag cccaggcagg
    11341 tgttgttgtt tgttgcagtt gtaataggta tgctcggaat catcatggca atttttccac
    11401 gcagtggtat tcggttaacg aataatttgg tattgtattt cccttcattt tcggagatgt
    11461 tttcaaaaaa agacgcaaga cttgatattg attcgctgct ctctaagcaa ttcaacatcg
    11521 attccttagg aatgaaggtc gacgacagtc tatctcctct tgatattgaa gaattgaaaa
    11581 agctaattac tcctcttgaa tttccgcaaa acaatccaca tgctctcgac ccgttttttg
    11641 ccaagcttaa caaccctgac gaagatggaa gaataagagt aatgcattac ggcgacagtc
    11701 agattgaagg cgataggatt actgcatttt taagaaacaa actccaatcg cgcttcggcg
    11761 gtctgggcat ggggctttca actgccggtg tggtttactc ccaatttagt atcattcagg
    11821 aaaactcttc aaactggaca agatacccgg gttttgttat gatcaaccct ctggtaaaac
    11881 ataaaaaata tggtgctatg atagccttta gtcggtttgc accaattgtc gactccggtg
    11941 cgacccttcc tgaaaccaaa tataaagcat ggctcagttt tacaaaatcg gatctggctt
    12001 attcaaacac aaagaagttt aaaaatgtgt caatttacta cgggaatgct aaagcgcggg
    12061 taaaaataag catcatcagt ggcgaaacag tgcttgttac cgactcgctg aaagcaggag
    12121 aagggatgac agtatttgaa tataaaagtt caacatatct cgaaaacatc aggtttgaat
    12181 ttgaaggaaa cgacagcccc gatttttatg caatttcatt tgaagacgac aagggggtgt
    12241 atatcgacaa catcgctttg cgtggcagca gcggaaccgt atttactact accgacggct
    12301 ccttgctttc gcaaatgttt acccgtcttg acgttgattt gtttatactg caattcgggg
    12361 gaaatgtgat gccctatatt aaagatgcta aaaatgccgc cttacatgca agctattttt
    12421 actctcagct cctttttctt aaaagattgg ttcccgatgc ctgcataatc gtaatagggc
    12481 catccgatat gtcgtacaaa gaaaaagatt actacaccac ctaccctatt ctcgaaaccg
    12541 taagaaacga acttaaggca gcaacattga aagctggagg tgtgtactgg gatatgtacg
    12601 aagctatggg aggtaaaaac tccatgatat cgtgggtaaa tgccaatccg ccattggcag
    12661 gtgccgatta tacccatttc acaccacaag gtacggttgt agttgcgaat atgttttaca
    12721 attcgttgat attggaatat tccgagtata atgaaaggat aaaagcaccc aaatgaaaaa
    12781 cataatcctt tcatttgtca tttgcacatc agtgatatat ggtgcatcag ggcagacaag
    12841 cagaattcca taccgaaact ataccttcgg atatattgaa agcgattctg tactattccg
    12901 cacctatggt gatagtagta ccataaactg tttctacgaa aaactcgacc ggcttatttt
    12961 cgaaggaagc ggaaatttgc acatcgtgca cttcggggca tcgcatattc aggcaggaat
    13021 ttggccatgg caaatacgat gcaattttga aaacatgagc agatgcatcg aaggaggtcc
    13081 gggatacgtg ttcccattca gtattgcaaa aaccaatcac ccttactatt accgttcgtc
    13141 atatgaaggg aactgggaaa ttgcaaaaat caccgatcaa aatgtttcgt gcccaatagg
    13201 tgttggaggg attactgcca tctgcagcga cagcattgca gaaattggaa ttcaattcaa
    13261 taagactgcc gacatataca aacacagctt cgataaagta agtgttttcc acagcgacat
    13321 aaaaggtacc tactccgtgt cattgattcc cgaatccgca gttaattatt ccacattcga
    13381 ttcagtttca ggtgctacag ttttttacct gaatagagag tgtgattcac tgaatctgct
    13441 ttttcaaaaa acggattcag gcgacgatac cttgtatttt tacggagcct acctcgtaaa
    13501 aatgactaat ggcattcact actcaggtat tggtataaat ggagcaagca ccatttctta
    13561 cttcaaagcc ggccgtttac agcagcagct gtgttgtatt gatcccgacc tgattgtttt
    13621 ttcgattgga gtgaatgatg cttcaggtaa gaactttacc ggagacaggt atgccgacaa
    13681 ttattgggtt ctgctttcca tggtaagaga agtttgtccg gcagcagcga taatccttac
    13741 caccaattcc gatttttatt attaccgtgg ttcacacaac gaacacgcag gagaagtgct
    13801 gcaagcaatg aaccaggttg ctgcagaatt cggagcctcg gtatgggact ttcacgcggc
    13861 aatggggggc aacaggtcaa ttaatttatg gaagcaggat ggacttgcac agcgcgacag
    13921 aatacatttt acacaggaag ggtataccat tcttgcccgg cttttcttcg aagccataca
    13981 aaaagatttt gaacaatata ttatcagaaa tgctttaaat cagcaataat atgagcataa
    14041 gtaatatctg ggatattttt gtttacgatc agaatgctcc actgattttt acccgctttt
    14101 tcttctgggg attttttgcc gtggttttgc tcggatatag ttttcttgca aagaaaaata
    14161 ctgcgcgatc attatatctg cttattttca gtctgttttt ctattataaa tcaagcggat
    14221 tctttttctt cttgctgata ttcagcacag tagtagacta ctacattggc aagtgggtat
    14281 ataaatcgaa cgacaacaaa aagcgaaaat ggctgttggc actgagtatt gttgtcaatc
    14341 tgggtgtgct cgcctacttc aagtatgcat atttcattgt tgaagctgca aaccagatgt
    14401 ttgggactca ttttacggca tataactacc tcgctgaatt ttcgaacttg ttaacgggct
    14461 ctcatttcga cacgtctaaa atattcctcc cagtagggat ttcttttttc acctttcaaa
    14521 ccatatctta cacagttgac atcttccgaa ggaaacttgc cccggtgaaa aacatcattg
    14581 acttcggatt ttatgtatcg ttctttccgc aacttgttgc aggaccaata gttagagcat
    14641 cggaatttgt accacagatt tatgaaaaat tcaggctcaa ccggtatgaa ttcggtatgg
    14701 ctttgttttt aattctaaaa ggtctgataa aaaaaatcat gattggcgac tacatcgccg
    14761 tgaattttgt tgaccggatt tttgcgaatc ccgacatgta ttccggcctt gaaagcgcac
    14821 tggcaatgat tggatattcg ctgcaggtgt acgtcgattt ttcgggctat accgacattg
    14881 ctatcggtgt tgcattgttg atgggcttcc gattgcccca aaactttaat tccccctaca
    14941 aggcaaaaaa ctgcagcgat ttctggtcgc gctggcacat gtcgctttcg tcgtggctga
    15001 aagattacct gtatataccg ctgggcggta accgtagtgc tagcccggga acctacataa
    15061 attttgcgct tataattgca gtatttgtgc tactagcagc aaatacatgg gttactatta
    15121 ttctgattgc attcacaata gtagttcttt tattaatgcg tttctcggta aaagcacgaa
    15181 agcaaataca aaccaatctt aacctaatga tcaccatgtt attagggggt ttatggcatg
    15241 gttcaagctg gaattttatg atatggggcg gacttaatgg tttgggagta ctggtgtaca
    15301 agtactggcg taaaataagt ccatgggagc acacaaacca ctggattgca accgtatgga
    15361 agattgctct tacattcacc ttcatcactt ttacacgcgt atttttcagg gcccccgatt
    15421 atacaactgc cgaaactatg ctaaaacgta tctggtattc attcaacggt tcggttttct
    15481 ttcaggtaat ggctgcatac tataaagtat ggctaatcat gttgtttggt tttgttaccc
    15541 attggctgag ctataaattc aaggataaat ggcgcgacag attcattggc atgccacact
    15601 gggctcaggc agttgtctgc acattggtgg tttttgtagt gtatcaatcg atatgttccg
    15661 acatgcaggc gtttatctat tttcagtttt aaaaaccatc ggtacatttt acctgaatta
    15721 aaaacagctt ttaaaaagtc aatctatttt cattttttta tgtaaacacc caaattttcg
    15781 attaaatttg catcctattt agtaaaaatt tgactaataa gtatttgaat taaaatttcc
    15841 tgattgttaa atataaatcc tattaaaatg agtaaaataa aacgcgttta cacttttgga
    15901 aataaaaacg ctgaaggcaa atcggacatg aaaaatttgc tgggtggcaa aggtgccaat
    15961 ctggctgaaa tgtgtttact gggtctgccc gtacctgcag gtcttactat taccactgaa
    16021 acctgcactg aatattatga aaacaactgt cagcttcccg acgggttaat ggaagatgta
    16081 tggaaaggca tggaactgat ggaaaaaaat atgggtatga aatacggcga caaagaaaac
    16141 ccgttgctcg tgtcgtgccg ttcgggtgct cgcaaatcga tgcccggtat gatggacacc
    16201 gttctgaata ttggattgag ctctgcaact ttgcccggct tgatcaagaa aaccaataac
    16261 ccacgttttg tttacgactc ttaccgccgg ttgattatga tgtatgccga tgtggtaatg
    16321 gacaaagccg aaggtctcga taaaaacatc cgtcgccagc tcgacgaaat gcttgatgaa
    16381 tataagcatg ccaaaggata taaatcggac accgacctct cgacagacga tttaatacat
    16441 ttatcggaag ctttcaagaa aaaagtaaaa gaagtgcttg gcactgaatt ccccgatgat
    16501 gccaaagaac agctcatcgg aggaattaaa gccgttttca aaagctggaa cggtaaaaaa
    16561 gctatttcgt atcgccgtat cgaaagtatt cccgataact ggggtacagc agtgaacgta
    16621 caagctatgg tgtttggtaa catgggcgat tcttcagcaa ccggtgttgc tttcacacgt
    16681 aatccggcaa ccggtgaaaa tttgttctac ggcgaatggc ttatcaatgc tcaaggcgaa
    16741 gatgtagtag ccggaatccg cactccaaac ccattgaaca gcgacaccaa aaacgaacag
    16801 aacaagcact tgcattcgat gcaggaattg taccctgtta tttataagga actctgcgat
    16861 attcgtgaaa cactggaaac cactttccac gatatgctcg atattgaatt taccattcag
    16921 gaaggaaaac tgtatatgct gcagtgccgt gtgggaaaac
//
LOCUS       DAOOQP010000061        16839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4152_length_16839_cov_5.000596, whole genome shotgun sequence.
ACCESSION   DAOOQP010000061 DAOOQP010000000
VERSION     DAOOQP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4152_length_16839_cov_5.000596"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLK75_07610"
     CDS             <1..299
                     /locus_tag="PLK75_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39152.1"
                     /translation="ALAAKYYNIPFYVAAPSSTYDKDCSNGSEIVIEYRTMDEVLFLN
                     GYDAEGYARRVVVANPGSEALNPAFDVTPAGLITGFITEDGIFTPQELETKYAR"
     gene            299..928
                     /locus_tag="PLK75_07615"
     CDS             299..928
                     /locus_tag="PLK75_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOY39153.1"
                     /translation="MDSNKHTEYRNEVAYWMRRLYARGLTTCSGGNISLKSGEFIYLT
                     ASQSDKARIRAGNICILTPDGENVCPELKPSIETGMHLAVYNSNPAVQAIVHAHPENL
                     SMLSCLNQPVKNVFTGEQRFILGEIGTAVYKIMGSKALAEEVAFLTQSHRAVIMQNHG
                     AITVGKNLFEAFDRMEVFERLASQVLFSLQKPPIRVLSETEIRDIDLFK"
     gene            complement(979..1833)
                     /locus_tag="PLK75_07620"
     CDS             complement(979..1833)
                     /locus_tag="PLK75_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY39154.1"
                     /translation="MKKIIVPIDFSPESLCGLKLAIIFANRLRADIQMVYVQTKQSSF
                     FGAEIEHEYEKAQEQFTKVLAEYKKDVFQECDFSFIIKKGRIYEEVVKQVEAFDNSVV
                     ICSTHGVSGFTRFFVGSNTYRIIEATDRPVISITNCQYIREIKKIVMPIDVSIETREK
                     VPLVARIAKAFGAEVHIVKVTSSTNEGIHNKLRLYSRQIRQYFDQNGIKYEKSLLVGD
                     NITEITIDYAKTIDADLIAIMTEQTTTLSNLLLGSYALQMLNESPIPVLSVTSKQLHH
                     VHSFTAGG"
     gene            1909..3015
                     /locus_tag="PLK75_07625"
     CDS             1909..3015
                     /locus_tag="PLK75_07625"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOY39155.1"
                     /translation="MSEFDSSKIEYIIGIMSGTSLDGLDIAACSFARDTEVWKYTVAA
                     AETVVYDAKWRHRLAGAQNLSSFELIQLHNEFGSYVGKSVADFCKRNKINPSKSWISS
                     HGHTVFHRPEQGVTLQIGSGIHIADAACMPVVYDFRTADVALHGQGAPLVPVGDKFLF
                     STYNAFLNIGGFANCSYFSEDRLIAFDICPANIVLNYFSSQAGKSYDKNGNIGRNGKL
                     NRIVLERLNSLPYYSLNPPKSLGREWVEQNIFPFFEDAGLSPEDCLRTYYEHIAVQIK
                     SVLNEYSNILVTGGGAHNSFLMELMNDDVVKPFVIPNKQCVDFKEAIVFAFLGYLKLL
                     GQPNVLSSVTGADYDHSSGIVVYPSNSKQFTVNK"
     gene            3072..4520
                     /locus_tag="PLK75_07630"
     CDS             3072..4520
                     /locus_tag="PLK75_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOY39156.1"
                     /translation="MDTRLALQQLYSETFGTEPSAIVPLRQSGSNRQYFRIQGNSESV
                     IGTYGNDVSENKAFFYLTKHFTTKNIPVPRLIAVSADNYFYLQEDIGDTSLFSLIEQS
                     HKEGKTYSPVIENYFKKVIEQLVLLQREASGGINFRLCYPYHSFGIDSVLYDLRYFVF
                     YFAKIFDLNYNELFLEKEFKRFAALLVADSNTCFMYRDFQSRNIFIKNSEVVFIDYQA
                     GRKGPPQYDLASLLYQAKAMIPENKRKELLEFYLDLSGIIDRHDRQEFTRKYYMVVCV
                     RLLQTLGAYGLRGIIEQKNHFIQSIPPAISNLKNMLPVFDLKKSFPEMHNLLLQATEI
                     SGLDEATSEQSTSKLNVLVHSFSYKKGAPRDYSGNGGGFIFDCRGLLNPGKLNEYSPL
                     TGNDKEVIRFLNNETEANQIVANACKIVAVNIDSYIERNFKNLMIGFGCTGGRHRSVY
                     CCNRLAKTLNKKYRDKIVINVWHKELKIKYSL"
     gene            4760..6472
                     /locus_tag="PLK75_07635"
     CDS             4760..6472
                     /locus_tag="PLK75_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOY39157.1"
                     /translation="MKHLAIFLSLAFTTILSMFAAPVNEQTARRVAYNFMNYQAVLVG
                     KSGFSGEISSLYICMKNGETYYYTINFDQGGFVIISGDDATVPVLAYSLDESFDPANV
                     PPAAAEWMSAYEKQYELMWENSTPVYEPYMELWANMLNNNFDMNKSVRSVSQLLTTRW
                     NQDYPYNMFCPYHMAGPGEHCYAGCVATAMGQVMKYYNYPATGRFTKTYFWGDYFTVD
                     FGATTYRWDEMTNVINNTSKEAIAELLYHCGVASEMNYGPDGSGTQLEYAWFALKQYF
                     RYRPDCKFLEKDDFTNADWKFLLKQDLDMAKPLIYRGTNDYGEGHAFVCDGYQDTSYF
                     HFNFGWGGYSNGFYYLDDINPHLSFYWGQAAIFNLNPYDAEYCNSMVYEQQSWTVTDG
                     SGPNYYWNNSECSWLIAPGGAEKIDLHFTEMNTEEGKDIVYVYDGADENAPLLGSFSG
                     FSIPADLHTTGGTAFIKFVTDSQNQFDGWGVEYAVNTTSIKANSIGSFGIIPNPASKY
                     VEFIFGNDCSSIVIENTQGKRIQSFEAEGNAVKINIENLTPGIYIVTFNCTDNIYTRR
                     LVVQ"
     gene            complement(6553..8856)
                     /locus_tag="PLK75_07640"
     CDS             complement(6553..8856)
                     /locus_tag="PLK75_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY39158.1"
                     /translation="MLLVCLAATRLVDAQKVGLVLSGGGSKGLAHIGVIRALEENNIP
                     IDYVTGTSIGAIIGGLYAIGYSPDEMEAIIQSEDFINWYKGNVPDNYQIYFKKKDDHA
                     EMLNLKFAVRDSSLVPVLPTNLVANQPMDLGIIEIYSRNSAGAGHDFDKLFVPYRCVA
                     TDIHRNKAVVFSKGDMGYAVRASMTFPFYFKPISIDSTLLFDGGIVNNFPYDVMQRDF
                     KPDVIIGSLVSSDTQMPDEDDILGQIENMIMNEQLSLSMPDSLGITIKSDFSKTGLLE
                     FSRLEEFSTIGYINTMNNMEMIKKKISRRIPLDEVNKRREIYKKQQPEFLIDNIIIYG
                     VDKYEQKYILNSLKQNKKLLTLEQFKKAYYTLVLDNQIESAQPKAVYNSETGYFDMYL
                     NVKKTKRYDLLIGANLSSGFANMAFIGANYKILGKYAYFLHANIYFGRLYSSLYLSAR
                     MDIPSETPLALKFSGSLNRWDYYKSSSRTFFEDIRPPYLIHHDYYLRGELETPMTNSS
                     KLSAGINTGLLTDNYFQINNFLQSDTADVTDLSYLRIFGYFEHNTLNYKIYPNKGTYR
                     NIGVSYYLGKEVNYPGSTSSASDTAFNTHYWLHVRAVNDSYLNLSKRFTLGLYGEFSA
                     CNIPLMNNYTSTVITAQGFYPTPHSRTIYLEEYRANVYFATGIKGIYKFTKRLDLRLE
                     GFLFVPYQKIIKQDIGNQIFKAYYSKPFYTDPISFLQPVVAATVVFTTPIGPASISAH
                     YYQKEGGRFYYLFHIGYILFNKKTEDY"
     gene            complement(9008..10012)
                     /gene="gap"
                     /locus_tag="PLK75_07645"
     CDS             complement(9008..10012)
                     /gene="gap"
                     /locus_tag="PLK75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOY39159.1"
                     /translation="MVIKVAINGFGRIGRNVFKIAHSRNDIRIVGINDLTDTKTLAHL
                     LKYDSTQGKFDEEISYTADSIIVNGKATKVSAEKSPLKIQWGETPDVVIESTGVFLNR
                     ESEKGGYGDHIKNGAKKVILTVPAKDEIDAMIVLGVNNDMLKPEHICVSNASCTTNCL
                     APVAKILNDKFGIENGLMTTIHSYTNDQIILDGPHKDLRRARSCAVSQVPTTTGAAKA
                     VGKVIPELKGKLDGMAVRVPTPTGSLVDLVVNLKKETTKEEVNAAMKEAAAGPLKGIL
                     EYTEDPIVSVDIIHNPHSSIFDAGCTMVIGKTVKILSWYDNEWGYSNRVVDLVPMLMK
                     "
     gene            10201..11004
                     /locus_tag="PLK75_07650"
     CDS             10201..11004
                     /locus_tag="PLK75_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39160.1"
                     /translation="MKLKYQAMHRLPIILLFTLLCFPCLSQCITARDYLFSGICKRTS
                     TPADLGGSVADFDTAILLQPAFAEAYANRGYSLYVNKDFYGAISDFSKAIQLSRSADS
                     VEWTIPLSVVGDMPGLYACYYLRGVAYFILEDSKSAINELTQAIQLFPEIADAYAFRG
                     DIKTFLTDYKSAVADYDMAIILNTQDPGYFEIYYRRGVAKAELSDYESAILDFGVCIE
                     MNPGFPAAYYYRGLSKLMISDSKGACLDLLHAEDMGFQQAAELRKKHCK"
     gene            complement(11044..11116)
                     /locus_tag="PLK75_07655"
     tRNA            complement(11044..11116)
                     /locus_tag="PLK75_07655"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11081..11083),aa:Phe,seq:gaa)
     gene            11300..14710
                     /locus_tag="PLK75_07660"
     CDS             11300..14710
                     /locus_tag="PLK75_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY39161.1"
                     /translation="MKNKILTLLICCIATLSFSQSHNRSKHPSVQVMNSAQGEIVLNI
                     ETGEYNLARVETPNGIQYIVNAEGASSLLHAAAPDIPCLSVSVIVPDNGSFTADVTYS
                     DFEDIENVLIAPSKGSLSRSIDPATVPYSYGSAYQNDAFFPGSDLPVLSDPFILRDFR
                     GITVMTYPFQYNPVTKVLRIYKNVSVRIVNNHSAQAVNELFRTKSLSVTDSDFDKLYN
                     DFFVNYRNNPLRYTSLEEGTPGNILILCKNTYLADMADYVQWKTEKGFHVELVDVAEA
                     GTNANAIKSFVLNYFNTNGITYLLLVGDSQHIPAVSASSGDSDNSYAYVLGSDGYADF
                     FVGRFSGESSANIQTQVQRTIQYERDLDASAIWLENAFGSASNEGQGQGHDGGESDVT
                     HMNNIKTDLENYGYTVTSVYQDGGNNAQITAAFNSGIGLANYIGHGDVDQWVNTTFTN
                     NNVNQLLNENKLPFIFSVACINGDFAGQTCFAEAWLRATKNGNPTGALAFLGSTINQS
                     WAEPMTAQDEMNDVLVESYNHNIKRTFAGIAFAGMFLMIEEGGQGQAMADTWSVFGDP
                     SVMVRTKTPQAMAVSHDPVFSVGATDFTVNCDVEGALVALSTLDGENTVLLGSAYVSA
                     GSAQVTIPAFSAPGTMKVTVTAYNRITYQGDVTIIVPDGPYVVLDQVLIDDSDANNNL
                     QIDYNEIIYLGLELTNVGVEVASNVVVTTSSESPLVDVTDNTQTIGDITVSETITEEW
                     AFTAVFSDGIADQTMIPFTLLISGDNENSWNTSYNLRVNAPSLAIDFIGVNDDGTGNG
                     NGLLDPGETVQLMFRLSNNGHSDALEGSLTYSVSGPCQTSGSPLQVDAMVAGGNGMYS
                     IGVTIDEEAESGSLIGCNLIYDAGAYSKSLAASVPVGLQIEDWESADLTSFAWQNDAS
                     HPWFITESNPYEGNNCLQSGDLPESGGESALILVVDVVADDNISFYKKVSCEPSSWGY
                     YWDFLAFYIDDVMKGQWAGTVAWSNQSYPVTAGLHELKWTYEKDSYQTNGSDCAWIDL
                     ITLPAHSQNVAIQYVTSDIEEFKMNMFPNPATDMVNISALLPENGIMSIEMYSVTGQY
                     VKTIVHEMDFNEGSAIFHFSVNELPAGFYIVVSRFGNTSHTMNLSVIR"
     gene            14721..16427
                     /locus_tag="PLK75_07665"
     CDS             14721..16427
                     /locus_tag="PLK75_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOY39162.1"
                     /translation="MRRISVLLALLVMPLFLFSQQPIQSSFEGSHHMLSHEELLPFYG
                     SKAFEETDPPVGNVRSIAEFETNSGVIVSRPSWGGFGIPLELIASLSQQVAVYVIVAN
                     SSANSSVLNSLDDAGCQMENITIININIDSYWSRDYSPWFIAYGDSFQIGIVDFPYNR
                     PQRVNDDQFPIEIADYFELPVFGMNLTHTGGNYMCDGLGVAASTKLLEAENDNLSTAE
                     INQLASDYLGIDHYHLIQDPLGEYIEHVDCWGKFLDVDKILISQVAEADDRYDDFESV
                     ANYWANQTTSYGNKYQVFRVYSPNGQPYTNSLIMNDKVYVALVTGTGSEWNDEALDVY
                     ENAMPGYEVIGMYNTTSSPWETTDALHCRTHEVPDFGMLYISHMPYYDTIDYSGSFTF
                     STEIQPYSGEQVVSESVKLHYKINNGSFVELAMQQALDNVWTCSVNSFDPGDVVDYYL
                     SASDESGRSATHPFIGSSDPHHFVIAEDSYSKTFLSSEFTVYPNPVRDRLTVIVEGNM
                     DGKLQYKIVDVYGKTMDYGELSISSEWQRSIINTDRLAPGVYMLVLENEGNVTSKRFI
                     VY"
     gene            16627..>16839
                     /locus_tag="PLK75_07670"
     CDS             16627..>16839
                     /locus_tag="PLK75_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOY39163.1"
                     /translation="MKNTTKIFVLDTNVLLHDHECLFKFQENDIIIPIVVLEELDHFK
                     RGNDLINFHAREFTRVLDELSGEELFE"
BASE COUNT         4836 a         3209 c         3602 g         5192 t
ORIGIN      
        1 aagccttggc tgcaaaatat tacaacattc ctttttatgt cgcagcgcca tcatcgactt
       61 atgataaaga ctgtagtaat ggaagcgaaa tagtgattga atacagaaca atggatgaag
      121 tattgttcct aaatggttac gatgctgaag gttatgccag acgagtggtg gtggcaaacc
      181 ccggatcaga agctttgaac ccggcgttcg atgttacacc tgccggactt attaccgggt
      241 ttattacaga agatggtatt tttacccccc aagaattaga aacaaaatat gcaagataat
      301 ggatagcaat aaacatacgg agtaccgaaa tgaggttgca tactggatgc gaaggcttta
      361 tgcccgggga cttaccacct gcagcggggg taatattagc ctcaaatcag gggaattcat
      421 ttatttaacg gcttctcaat ccgacaaagc tagaatacga gcagggaaca tatgcatttt
      481 aacccccgat ggagaaaatg tctgccctga attaaaacca agcattgaaa caggaatgca
      541 tcttgcagtt tataattcta accctgcggt ccaggcaatt gtgcatgcac atcccgaaaa
      601 tctttcaatg ctatcatgtc tcaatcaacc ggtgaaaaat gtattcaccg gagaacaaag
      661 gtttattctt ggcgaaattg gaacggctgt atataaaatt atgggaagta aggcactggc
      721 agaggaagtt gcttttctta cccaatcgca tcgcgctgtc attatgcaaa atcacggtgc
      781 aatcactgtt ggcaaaaacc tttttgaagc attcgacaga atggaagtat ttgagcgatt
      841 ggcgtcacaa gttttatttt cgttacagaa gcccccgatt cgtgttctga gtgaaacaga
      901 aatcagagat attgatttgt ttaaataaaa aaatgaggag aaatcctcat tttttattat
      961 ttgtttccat ctgaaatgtt aaccgcctgc agtaaaactg tgtacgtggt gtaattgctt
     1021 ggacgttacg ctcaaaacgg gtattggaga ttcgttgagc atttgaagag catagctgcc
     1081 aagaagcaaa ttcgacaagg tagttgtttg ttcagtcatt atggcaatca ggtctgcatc
     1141 gatggtcttt gcataatcga tagtaatttc tgtaatattg tccccaacta aaagactctt
     1201 ttcgtatttg ataccattct ggtcgaaata ttgccgtatc tgacggctgt ataaacgtag
     1261 tttattgtga ataccttcgt tagtagaaga ggtaacttta acgatatgaa cttcagctcc
     1321 aaaagctttg gcaatgcggg ccaccaaagg cactttttcg cgggtttcga tacttacatc
     1381 aattggcatt actatttttt ttatctctct gatatactgg cagttggtta tgctgataac
     1441 cggccggtcg gttgcttcaa ttatgcggta ggtgttgctt ccaacaaaaa aacgtgtaaa
     1501 ccctgatact ccatgtgtgg aacatattac cactgaatta tcgaaggctt caacctgttt
     1561 tacaacttct tcgtatatac ggcctttttt aataataaaa gaaaagtcgc attcctgaaa
     1621 gacatctttc ttatattctg caagaacctt ggtgaattgt tcctgtgctt tttcgtattc
     1681 gtgctcaatt tctgcaccga aaaacgaact ttgctttgtt tgcacatata ccatctgaat
     1741 gtcagcccgg aggcgattcg caaaaataat tgccaatttc aggccgcaca acgattcagg
     1801 tgaaaagtca ataggtacga taattttttt cattagtgtt tcttattttc tgctaaaata
     1861 aaaaaataaa catgatttat ctaattttgt gttacaaaaa tttttgtaat gtcagaattt
     1921 gattccagta agatagaata tataattggt attatgtcgg gcacctccct cgatggtttg
     1981 gatattgccg cttgctcttt tgcccgtgat actgaggtat ggaaatatac cgttgctgca
     2041 gcagaaaccg ttgtttacga tgctaaatgg aggcataggc ttgcaggggc tcagaatcta
     2101 tcatcatttg agcttattca actgcacaat gagtttggaa gctatgttgg caaaagtgtc
     2161 gccgatttct gcaaaagaaa caaaataaac ccatcgaaaa gctggatatc aagtcatgga
     2221 catacggttt tccaccgtcc ggagcaagga gttactttac agattggtag tggaattcac
     2281 attgcagatg ccgcttgtat gcctgtggta tacgatttca gaactgccga tgttgctctt
     2341 catggacaag gggctccttt ggtgccagta ggtgataagt tcttgttttc gacatataac
     2401 gcattcctta atataggtgg ttttgcgaat tgctcatatt tcagtgaaga caggcttatc
     2461 gcgttcgata tttgccctgc aaatattgtt ttgaattatt ttagttcaca agcaggaaag
     2521 tcttacgata aaaatgggaa tattggaaga aatggaaaac ttaatcgaat tgtcctggaa
     2581 cgtttgaata gccttccata ctacagtctg aatcctccca aatcgctcgg cagggaatgg
     2641 gtcgaacaaa atatttttcc gttttttgag gatgctgggc tttcacccga agactgcctg
     2701 cgtacatatt atgagcatat tgccgttcag ataaaaagtg ttttaaatga atacagcaac
     2761 attttggtta ccggtggtgg tgctcataat agttttttaa tggaattgat gaatgatgat
     2821 gtagttaaac cctttgttat acccaacaag caatgcgtcg attttaagga ggccattgta
     2881 tttgcatttt tgggctactt aaagctgctt ggtcaaccga acgtattgtc gtcggtaacc
     2941 ggtgccgact acgaccatag ttcgggtatt gttgtgtatc catcgaattc taaacaattt
     3001 accgtgaata aatgacctgt aacgagcatt ttaatttgcg ttgctggtgc tatttttaat
     3061 caaaaaaaat tatggacacg aggcttgctt tacaacaatt gtattccgaa acattcggca
     3121 ctgaaccttc agccatcgtt cctttacggc aatctggttc caatagacag tactttcgca
     3181 ttcaggggaa tagtgaaagc gtgattggta cctacggtaa cgacgtttcc gaaaataagg
     3241 cgttctttta tctgacaaaa cactttacaa caaagaatat tccggttccc cgacttatag
     3301 cagtaagtgc cgataattat ttttatttgc aggaagatat tggtgataca tcgcttttca
     3361 gcctgattga gcaatcgcac aaggagggaa aaacatactc cccggtgatt gagaattact
     3421 ttaagaaagt cattgaacaa ttggtactcc ttcaacggga ggcctcagga gggataaact
     3481 ttcgcctgtg ttacccatat catagctttg gaattgattc ggtgctgtac gatttacgat
     3541 attttgtgtt ttattttgcc aagatttttg atttaaacta taatgagcta tttctggaaa
     3601 aggaatttaa gcgatttgct gcactgctgg tagcggatag caacacttgt ttcatgtaca
     3661 gggacttcca gagcaggaat atttttatta aaaacagcga agtagttttt atcgattatc
     3721 aggcagggcg aaaaggacca cctcaatacg atctggcatc gttactctat caggcgaaag
     3781 ccatgattcc cgaaaacaag cgcaaggaat tacttgagtt ttaccttgat ttgtcgggaa
     3841 ttattgatcg acatgatcga caagagttta cccggaaata ttacatggtt gtttgtgtaa
     3901 ggcttttgca aactctggga gcttatggtc tgcgtggtat tattgaacaa aaaaaccact
     3961 ttattcagag tattccacct gcaattagta atctgaagaa tatgctgccg gtatttgatt
     4021 tgaagaagag cttccccgaa atgcataact tattgctgca agccaccgaa atatccggac
     4081 tggatgaagc taccagtgag caatcaactt caaaattaaa tgttctggtt cacagttttt
     4141 catataaaaa gggtgcgcca cgcgattact ccggaaatgg agggggtttc attttcgact
     4201 gcaggggttt attgaacccg ggaaaattaa atgaatatag tcctctgacg ggaaacgaca
     4261 aagaagtaat acggtttctc aataacgaaa ccgaagccaa ccagattgtt gcaaacgcat
     4321 gcaagattgt ggcagtaaat attgattcat atattgaacg aaattttaaa aacctgatga
     4381 taggctttgg ctgcacaggt gggagacatc gaagtgttta ctgctgtaat cggttggcta
     4441 aaacactcaa taagaaatat agagataaaa tagtaataaa tgtatggcac aaggagctta
     4501 aaatcaagta ttccttgtaa gaatgaattt aatcatttca ttatcgtaaa ctttgttgtt
     4561 tgagtaagcc ttttcttatt ccaattattt attgaaaagc cttatattca gttcgtatag
     4621 tctgtacttg taagttttta tgtttttaac tgtttctaaa gaatagttat tgaatgggct
     4681 gatatttatc tgataaaaaa aaatcaatag aggtttcttt gtttcgaaaa agattaaatt
     4741 tgcccaaaaa ttacaaataa tgaagcactt agccatcttt ttatccctcg cttttacaac
     4801 aatcttatca atgtttgcag ctcctgtaaa tgagcaaaca gcaagacggg ttgcatataa
     4861 tttcatgaac taccaggctg ttttggtcgg taaatcaggt ttcagcggcg aaatatcctc
     4921 cctttatatt tgcatgaaaa atggggaaac atattattac actataaatt ttgatcaggg
     4981 tggttttgtg attatcagtg gagatgatgc aactgtaccg gttctggcat attcgctcga
     5041 tgaaagtttt gaccctgcaa atgtacctcc tgcagccgct gaatggatgt cagcctacga
     5101 aaagcaatat gaacttatgt gggagaatag tactccggta tatgaaccat atatggaatt
     5161 gtgggcaaat atgcttaata ataattttga tatgaacaaa agtgttagat ctgtttcaca
     5221 actgcttaca acgcgctgga atcaggatta tccatataat atgttctgtc cataccatat
     5281 ggccggcccc ggagaacatt gctacgccgg ttgcgttgct actgcaatgg gtcaggtaat
     5341 gaaatactat aactacccgg ctaccggtag gtttactaaa acctatttct ggggcgatta
     5401 tttcactgtg gattttggtg ccacgactta tcgctgggat gaaatgacga atgtaatcaa
     5461 caataccagt aaagaagcaa ttgccgaact cctataccat tgcggagtag catctgaaat
     5521 gaactatggc cctgatggtt ctggtactca gctcgagtac gcatggtttg ctctgaaaca
     5581 gtattttcgc tatcgccccg attgcaaatt cctcgaaaaa gacgacttca ccaatgccga
     5641 ctggaaattt ttattaaagc aggatttaga tatggcaaaa ccgttaattt accgtggtac
     5701 taacgattat ggcgaaggtc atgcttttgt ttgcgacggt tatcaggata catcatattt
     5761 tcatttcaat tttggatggg gtggttatag caatggattt tattatctgg atgatatcaa
     5821 cccacatttg agtttttatt gggggcaggc agctattttt aatctaaacc cttacgatgc
     5881 agaatattgt aactcgatgg tttacgagca gcagtcgtgg acagttaccg atgggagcgg
     5941 accaaattac tactggaata actcagaatg ttcttggcta atagcccccg gaggagccga
     6001 aaaaattgat ttacacttta ctgaaatgaa tactgaagag ggaaaggata ttgtgtatgt
     6061 gtatgacggt gccgatgaaa acgctccatt gctggggtca ttttcaggat tttcaattcc
     6121 agccgattta catactaccg gcggaaccgc gtttataaaa tttgttaccg atagccaaaa
     6181 tcagtttgat ggatggggtg ttgaatatgc agttaacact acgtcaatca aggctaatag
     6241 cattggaagt tttggtataa ttcctaatcc ggcatcaaaa tatgttgaat ttatatttgg
     6301 aaacgactgc agttctattg tcatcgaaaa cactcagggt aaaaggattc aatcgtttga
     6361 agctgagggg aacgctgtta aaattaatat cgaaaatcta actccaggta tttacatcgt
     6421 tacttttaat tgtactgata acatatatac ccggcgtttg gttgtgcaat aagttcatgg
     6481 tctaaaaatc aaaatcttca ttcactcccc gggataattg gattttccta catttgctgt
     6541 aataagttgg ctttagtaat cttctgtttt tttgttgaaa agaatgtagc cgatatgaaa
     6601 caggtaataa aacctgccgc cttctttctg gtaataatgt gcacttattg acgcaggtcc
     6661 gatgggagtt gtgaaaacca ctgttgctgc cacaactggc tgtagaaatg aaattggatc
     6721 ggtatagaaa ggcttactat aatatgcttt gaaaatctga ttcccgatat cttgttttat
     6781 tattttctgg tagggaacaa ataaaaaccc ctctaatctt aaatcgagtc gttttgtgaa
     6841 tttataaatg ccttttatac ctgtggcaaa atatacattg gcacggtatt cctccaaata
     6901 aattgttctg ctgtggggag taggataaaa cccttgtgca gttatcaccg ttgatgtata
     6961 gttgttcatg agaggaatat tgcatgcaga gaactctcca tacaatccca gggtaaatct
     7021 ttttgaaagg ttcagatagc tatcgtttac agctctaacg tgcaaccagt aatgggtgtt
     7081 gaaagctgtg tcggatgctg atgatgttga cccggggtag ttcacttcct ttccaaggta
     7141 gtacgatact ccaatattcc tgtaagttcc tttgttggga tatattttgt agttcagtgt
     7201 attatgttca aagtatccga aaatgcgcaa atacgacaaa tctgtgacat cagcagtgtc
     7261 agattgaagg aaattgttga tctgaaaata gttgtcggtc agcagtccgg tattaatacc
     7321 cgccgatagt ttactactgt ttgtcatagg tgtttccagc tcgccccgca ggtaataatc
     7381 gtgatgaata agatacgggg gtctgatatc ctcgaaaaaa gtccgtgagc tgcttttata
     7441 ataatcccac cggttgagtg atccggaaaa ctttagagcc agaggcgttt ccgatggtat
     7501 atccatccgg gctgaaagat ataaagatga ataaagtctt ccgaaataaa tattcgcatg
     7561 gagaaaatag gcatatttcc ccaggatctt ataatttgct ccgataaaag ccatgtttgc
     7621 aaatcccgat gataaattgg ctccgataag caggtcgtag cgtttggttt tttttacatt
     7681 cagatacatg tcgaaatatc cggtttcgct gttgtaaaca gcttttggct gagccgactc
     7741 aatctggttg tcgagtacca aagtgtaata tgcttttttg aattgttcca gagtgagaag
     7801 ctttttattt tgcttcaggc tattcagtat gtatttctgc tcatatttgt cgacaccgta
     7861 tatgatgata ttatcaatga gaaactcagg ttgttgtttt ttgtatattt ctcgtctttt
     7921 gtttacttca tccagcggaa tgcgtcgcga tatcttcttt tttatcattt ccatgttgtt
     7981 catggtattg atatacccaa tcgtggaaaa ctcttcgagc ctcgaaaact ccagtagtcc
     8041 ggttttcgaa aagtctgatt ttatggttat ccccagagag tcgggcatgc taagtgaaag
     8101 ttgctcattc attatcatgt tttcgatctg ccccagtata tcatcttcat ccggcatttg
     8161 ggtatcgctg cttacaaggc tgccaatgat tacatcgggc ttaaaatcgc gctgcatcac
     8221 atcgtaaggg aaattgttga caatacctcc atcgaatagt aatgtgctgt cgatggaaat
     8281 gggtttaaag taaaacggaa acgtcatgga ggctctcacc gcataaccca tatcgccttt
     8341 agagaaaact accgctttgt ttcggtgtat gtcggtggca acgcagcgat aaggtacaaa
     8401 taatttgtcg aaatcgtgtc cggccccggc actgttgcga gaataaatct caattatccc
     8461 gaggtccatg ggttgattgg ccactaaatt tgttggaagc accggaacca gcgaagagtc
     8521 tctgacggca aatttcaggt ttaacatttc ggcgtgatca tcttttttct tgaaataaat
     8581 ttgatagtta tcaggcacat tccccttata ccagttaata aaatcttctg attgaattat
     8641 tgcctccatt tcatcgggag aataacctat ggcatacaag cctccgatga tagccccgat
     8701 agaagtgcct gtaacgtaat cgatgggaat gttgttttct tcgagtgctc tgattactcc
     8761 aatgtgagcc aaccccttcg atccgccacc actaagcaca agaccaacct tctgtgcatc
     8821 tactaacctg gtggcagcaa gacaaactaa taacaacagt attattttta tcgaaaaacg
     8881 cggcataaag tcaattcctg agtacaaaaa tatttaaaaa ccttaacatg aaggatgcgt
     8941 ttgaaaaaaa tgtttattca aaaaaaggca gttcgtcgac tgcctttttt tataaattgt
     9001 gattaaatta tttcatcagc atgggaacca gatccacaac gcggttactg taaccccatt
     9061 cattgtcgta ccaggagagg attttcacgg ttttaccaat caccattgtg catccggcat
     9121 caaaaattga tgagtgtggg ttgtgaatga tatcaaccga aacgattgga tcttcggtat
     9181 attcaaggat tcccttaagc ggtcctgcag cggcttcttt catagctgca tttacttctt
     9241 cttttgtagt ttcctttttc aaattaacta caaggtcaac caacgacccg gttggagtgg
     9301 ggacccttac agccattcca tcgagtttgc cttttaattc agggattacc ttacccacag
     9361 cttttgcagc acctgtggtg gtgggaacct gtgacacagc gcacgagcgg gcacggcgca
     9421 ggtctttgtg aggtccgtcg agaattatct gatcgtttgt gtaactatgt atggttgtca
     9481 tcagaccgtt ttcgataccg aatttatcgt tcagtatttt tgcaaccgga gcaaggcagt
     9541 tggtggtgca gcttgcattc gatacgcaaa tatgttcagg ctttagcata tcgttattca
     9601 ctcccagaac aatcatagca tcgatctcat cttttgcagg tactgtaagg atcacctttt
     9661 ttgcaccgtt cttaatatgg tcgccatagc cgcctttttc gctttctcgg ttcaggaaaa
     9721 caccggttga ttcaattaca acatcaggcg tttctcccca ttgaattttt aatggcgact
     9781 tctcagcact aactttggtg gctttaccgt taacaattat cgagtcggca gtatagctga
     9841 tttcttcgtc gaatttgccc tgagttgaat cgtatttcag caaatgagcc agtgttttgg
     9901 tatcggtcag atcgttaatg ccaacaatcc tgatatcgtt acgcgaatga gcaattttaa
     9961 aaacattgcg ccctatgcgc ccaaatccgt taatagctac tttaattacc atgatactat
    10021 agttttattg tttgattcat gcaaaattaa taaaaacctg tattgaatat aaatttgtga
    10081 tatgttatat aatctgattt tgtaataaat tgaatggtaa tatgttagtt agtggtttta
    10141 ggattattag gttatgattg ttgtttgagc aatgattaat tttccgaaaa ttaattatat
    10201 ttgaaactca aataccaagc aatgcatcgg ctgccaataa tactactatt taccctgctg
    10261 tgtttccctt gcctttcgca gtgcatcaca gcccgcgatt accttttttc tggaatatgt
    10321 aaacgtacat ccacaccggc cgaccttggg ggatcggttg ctgattttga taccgccatt
    10381 cttcttcagc ctgcttttgc cgaagcatac gcaaaccgtg ggtattcgtt gtatgtaaat
    10441 aaggattttt acggggctat tagtgatttt agcaaagcaa ttcaattgag cagatcagcc
    10501 gattcggttg agtggacaat accattgtcg gttgttggtg atatgcccgg tttatacgct
    10561 tgctattatt tacgaggcgt tgcttatttc attctcgaag actctaaaag tgcaattaac
    10621 gaattaacac aagccattca gctttttcct gaaatcgccg atgcatatgc attcagaggt
    10681 gatataaaaa cgtttcttac cgattataaa tctgcagtag ccgattacga tatggcaatt
    10741 attttgaata ctcaagaccc cggatatttt gaaatatact acagaagagg cgttgcaaaa
    10801 gcagaattaa gcgattatga gagtgccatc ctcgactttg gagtctgcat tgagatgaac
    10861 cccggttttc cggcagcata ttattatcgt ggtctttcga aactgatgat ttccgattca
    10921 aaaggtgcat gtctcgattt attgcatgct gaagatatgg ggtttcaaca agcagcagaa
    10981 ctgcgcaaaa aacactgtaa ataaaaagcc ttgtaatact tcgtttacaa ggctttgcgc
    11041 ttgtggtatc acctggaatc gaaccaggga caccaggatt ttcagtcctg tgctctaccg
    11101 tctgagctat gataccaaaa cagcggtgca aatataaatc aattttttta ttatgcaaac
    11161 aattgaaaaa tattcaaacg tacttgaata tgtagttact taaaaatgct ggtggattag
    11221 atttttatag taaaattttt ctctgaaata cattgactat tactattttg atatatttgt
    11281 tgtttatgaa atttacatta tgaaaaacaa aattttaact cttttaattt gttgtatcgc
    11341 aacattaagc tttagtcaaa gccataacag aagtaagcac ccatcagttc aagtcatgaa
    11401 ctctgctcaa ggtgaaatag ttctgaatat tgaaaccggt gagtataacc ttgcgcgcgt
    11461 tgaaaccccg aatggtatac aatatatcgt taatgccgaa ggtgcatctt cattgctgca
    11521 tgcggccgca cccgatatcc cttgtttatc ggtatcggta attgtgcctg ataacggaag
    11581 ttttactgcc gatgttactt actccgattt tgaggatatc gaaaatgtgt taattgcccc
    11641 ttcaaaaggt agcctttcac gtagcattga tccggcaaca gttccatata gctatggtag
    11701 tgcttatcag aacgatgctt ttttcccggg aagtgatctg cccgtattaa gcgacccttt
    11761 tattttaaga gatttcaggg ggataacagt aatgacgtat ccgtttcagt ataatccggt
    11821 aacaaaagta ctccgcattt acaaaaatgt aagtgtccgt atcgtcaata accattcggc
    11881 ccaggcagtg aatgagttgt tcagaactaa gtcgctttct gtaacagata gtgatttcga
    11941 taagctgtat aacgacttct ttgtcaatta tcgaaataat cctttgcgtt atacttctct
    12001 tgaggaaggt acaccgggca atattctaat cctttgcaaa aacacatact tagctgatat
    12061 ggctgattat gtgcaatgga aaaccgaaaa aggatttcat gttgagctgg ttgatgttgc
    12121 cgaagccggt actaacgcta atgcaattaa atcttttgtg ctgaattatt ttaataccaa
    12181 cggtataaca tatctattgc tggttggcga ttctcaacac attccggctg tttcagcaag
    12241 ctccggcgat tccgataatt cttatgctta cgtgttgggg agcgatggat atgccgactt
    12301 ttttgttggt cgtttttcgg gggaatcttc agccaatata caaacacagg tacaacgcac
    12361 cattcaatac gaaagggatt tggatgcttc ggctatctgg ctcgaaaatg catttggttc
    12421 agcatcgaat gagggccagg ggcaagggca tgatggtgga gagtcggacg ttactcatat
    12481 gaataatatc aaaaccgatc ttgagaatta tggctatact gttacaagcg tatatcaaga
    12541 cggcggcaat aatgcacaga taaccgcagc attcaattca ggtattggac tggcaaatta
    12601 tatcggacat ggtgatgttg accaatgggt aaacactaca ttcaccaaca acaatgtaaa
    12661 tcagctactt aatgaaaaca agctcccgtt tatatttagc gttgcttgta taaatggcga
    12721 ttttgccggt cagacatgtt ttgctgaggc ttggttacgg gcaacaaaaa acggaaaccc
    12781 aaccggtgct cttgccttcc tgggttcaac catcaatcag tcgtgggcag aacctatgac
    12841 agcacaggat gagatgaacg acgtactggt agaatcgtat aatcataaca ttaaaagaac
    12901 atttgcagga atcgcttttg caggaatgtt tctgatgatt gaagaaggag ggcaggggca
    12961 agcaatggcc gatacgtggt cggtatttgg tgacccttcg gtaatggttc gcactaaaac
    13021 tcctcaagcg atggctgttt ctcatgatcc agtattctca gtcggtgcaa ctgacttcac
    13081 agttaattgt gatgttgaag gggctttggt tgcattaagc acattggatg gagaaaatac
    13141 tgttttactc ggctcagctt atgtttcggc aggcagtgca caggtaacaa tccccgcttt
    13201 ttctgcaccc ggtacaatga aagtcactgt tacagcctat aatagaataa catatcaggg
    13261 cgatgtaaca attattgtcc ccgacggccc atatgtagtt ctcgatcagg ttttgattga
    13321 tgattccgat gcaaataata atttgcagat cgactataat gaaattattt atttgggact
    13381 tgagcttacc aatgtcggag ttgaggtggc ttccaatgtt gtagttacaa cttcttccga
    13441 aagcccgctt gttgatgtaa ccgacaacac tcaaactatt ggtgatatca cggtttcgga
    13501 aacaatcacc gaagaatggg catttactgc tgtgttttct gacggaattg ccgaccaaac
    13561 aatgattcca ttcactttgt taatttcagg tgataatgaa aacagttgga atacttcgta
    13621 taatttacgg gtgaatgcac cttcgctggc tatcgatttt ataggagtta acgatgacgg
    13681 tacaggaaac ggaaatggtc ttcttgatcc gggcgaaaca gtacaactca tgttcaggct
    13741 gtcgaataac ggacattccg atgcattaga aggctctttg acttacagtg tgagcggacc
    13801 ctgtcagact tccggttcac cgctgcaggt tgatgcaatg gttgctggtg gcaatggaat
    13861 gtatagcatt ggtgtcacta tcgacgagga ggctgaaagc ggtagcctga tcggctgtaa
    13921 ccttatttac gatgcgggtg cgtattcaaa gtccttggca gcatccgttc cggttggttt
    13981 gcagattgaa gactgggaaa gcgccgatct tacctcgttt gcatggcaaa acgatgcttc
    14041 gcatccttgg ttcattaccg aaagcaaccc atatgaaggt aataattgcc ttcagtcagg
    14101 cgatttaccg gaatcaggcg gagagtctgc cctgattctg gttgttgatg ttgttgccga
    14161 cgataacata agcttttata agaaagtgtc atgcgaacct tcatcgtggg gttattactg
    14221 ggatttcctg gcattttaca tcgatgatgt aatgaaaggg cagtgggcgg gaaccgtagc
    14281 atggtcgaat cagtcctatc cggttacagc aggtctccat gaactgaaat ggacatatga
    14341 aaaagacagc taccagacca atgggtccga ctgtgcatgg atcgatttaa ttacactccc
    14401 ggctcattca cagaatgtgg ctattcaata tgttacctct gatatcgaag aattcaagat
    14461 gaacatgttc ccaaacccgg caactgatat ggtaaatatt tctgctttgc ttccggaaaa
    14521 tggaattatg agcattgaga tgtattcggt tactggacag tatgttaaaa caattgtgca
    14581 tgaaatggat tttaacgagg ggagcgcgat atttcatttt tcggtcaatg aactccctgc
    14641 cggtttttat attgttgtta gccgttttgg aaacacaagc catacaatga acctgtcggt
    14701 aatccgttaa atatttatcg atgagaagaa tatctgttct gcttgcgctg cttgttatgc
    14761 ctttatttct tttttcacag cagccgatac aatcgagttt tgagggtagc caccatatgc
    14821 tgagccacga ggaactgttg cctttttatg gttctaaagc atttgaagaa accgaccctc
    14881 cggtcggtaa tgtcagaagc attgctgaat ttgagacaaa ctctggggta atagtcagcc
    14941 ggccctcttg gggcggattt ggtatccccc ttgagctaat agcaagccta tctcaacaag
    15001 ttgccgtgta tgtaattgtc gccaactctt ctgcgaatag ttctgtgttg aattcgcttg
    15061 atgatgccgg ttgccagatg gagaacatta ctattattaa cattaatatc gattcatatt
    15121 ggtcgcgtga ctacagccct tggtttattg cctatggcga ttcatttcag ataggaattg
    15181 ttgatttccc atacaatagg cctcaacggg tgaacgatga ccaattcccg attgaaatag
    15241 ccgattactt cgagcttccg gtttttggaa tgaaccttac ccatacaggg ggcaattata
    15301 tgtgcgacgg cctgggcgta gctgcaagta caaaactcct tgaagcggaa aatgacaatt
    15361 tgagtactgc agaaataaat cagcttgcct ctgattatct aggcatcgac cactatcatc
    15421 tgatacagga cccattgggt gaatatattg agcatgtaga ttgctggggt aaattccttg
    15481 atgttgataa aatactcatt tcacaggttg ccgaagccga cgatcgttac gatgattttg
    15541 agtctgtagc caattattgg gctaatcaaa ccacctccta tggtaacaaa tatcaggtgt
    15601 ttagggtata ctcacctaat ggacagccct ataccaattc cttaattatg aacgataagg
    15661 tttatgttgc actggttacc ggaactggat ctgaatggaa tgatgaggct ctcgatgttt
    15721 atgaaaatgc catgccgggt tacgaggtta ttggtatgta caatacaaca tcttcgccat
    15781 gggaaacgac cgatgcattg cactgcagaa ctcatgaggt tcccgatttt ggaatgcttt
    15841 acatcagcca tatgccgtac tacgatacaa ttgattattc gggtagtttt acattttcta
    15901 ccgaaataca accctattcc ggagaacaag tagtaagtga atcagtgaaa cttcactaca
    15961 aaatcaataa tggaagtttt gtagaactgg cgatgcagca agcattggat aatgtttgga
    16021 cttgttcagt gaacagcttt gatccgggag atgtggtgga ttattacctt tcagcctccg
    16081 acgaaagcgg acgttctgca acgcatcctt ttattggtag ctccgacccg catcattttg
    16141 ttatagcaga agacagttac agcaaaacct ttttgtcatc agaattcact gtatatccaa
    16201 atccggttcg ggacagactt actgttattg tggaaggtaa tatggatgga aagctgcaat
    16261 ataagattgt tgacgtttat ggtaaaacaa tggattatgg tgaattgagt atctcctccg
    16321 aatggcagcg aagtataatc aacaccgaca ggctggctcc gggtgtttat atgctggtgt
    16381 tggaaaacga aggaaacgtc acatcaaagc gtttcattgt gtactgatga attttaacac
    16441 cgaagcggtt accaagcgca gtcgaggtgc ccactatttg aaaacaatct ttttaatcca
    16501 gttagcatca agccgacatc tcgactactc gatgtactcg atgtacagct gaataaaaat
    16561 taaaagataa cattttctga tatcttattt tattggtatc tttaagcaac aatttttaaa
    16621 cgtataatga aaaacacgac caaaattttt gttctcgaca caaatgttct gctacatgat
    16681 cacgagtgcc tgtttaaatt tcaggaaaac gatattatca ttcccattgt tgttcttgaa
    16741 gagttagatc attttaaacg tgggaacgac ctgattaatt ttcacgcccg tgagttcaca
    16801 cgtgtattgg atgagctctc cggggaggaa ttgtttgaa
//
LOCUS       DAOOQP010000062        16618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4226_length_16618_cov_5.521584, whole genome shotgun sequence.
ACCESSION   DAOOQP010000062 DAOOQP010000000
VERSION     DAOOQP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_16618_cov_5.521584"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2439)
                     /locus_tag="PLK75_07675"
     CDS             complement(97..2439)
                     /locus_tag="PLK75_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOY39164.1"
                     /translation="MIKIGSLVLLFVSVLSLQITAQSTQSYLSVDLGSKGILALHEIG
                     VEPVHGFLKKNGEFTGVFEGYDVMKIEESTLQYEVIIEDLVKYYQFRGNNAAKEEVFN
                     ECFNTVQNYPLPEGYSHGSMGGYYTFDETIAELDQMRSLYPNLISVKTQIGTSIEGRP
                     IYSVKISDNPDISEDEQQVLYTSLHHSSEPCSLQQLIFFMFHILENYGTDPEITYLID
                     NTEMYFVPAVNPDGYVYNETENPGGGGDWRKNRRPVDYMFQTYYGVDLNRNYGYAYAY
                     DNIGSQPLVWSPWYRGENAFSEPESFAMSQFLIAHDIKLQVNWHAAGNMLIYPWSYIN
                     ESTPDSLLFLTICEKMTEDNHYRYGNVGETYGYQSNGDADDWSYGEVGVKNKILSMTA
                     EIGTLDDGFWPSIERIDDLCRGAVRLNLNMAHFSQAFYMFDDISPDIINHLSGNLDFT
                     VECVGLDTPSNFTLTFIPLSGNISFQNNTQVFDNMTLLEKRSGQIAYTLDAAYGDLIS
                     YIVAGNNGIYTFSDTVTKTYGPVTTLFYDDCETMEQWSGDDFGISNSVFYAGSGSITE
                     SPDGNYELFQNSEIVTNDNINLENISVAVLTFKVRFDIENTHDYCEIFVSADNGENWQ
                     PLCTKLSRVGSDDQDEGQPVYTGIKTIWQTDEVDLQDYLGESIKIKIKFVSDQSITHD
                     GIYFDEIYVLGIDTTALRSGIIANSSVTVHPNPAQSGFLVHSGCDQGIISSYNAEAKQ
                     IYQSVIAKGDTWIPAEQWESGLYFYRFTGCENASGKLIKE"
     gene            2597..3808
                     /locus_tag="PLK75_07680"
     CDS             2597..3808
                     /locus_tag="PLK75_07680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY39165.1"
                     /translation="MKSINRNIYMLYLIKVAKWFMLVMPIIVLFYQSHGLSLRDVLTV
                     QAVYSVSIVLWEIPSGYAADYWGRRKTIILGSILGTIGFVIYSFANGFWGFIAAESVL
                     GIGISLISGADSAILYDSLVEKGRQNEYMKLEGRVISIGNFSETLAALAGGLLAEISL
                     RTPFIAQTFVAFIAVPASFLLYEPARTKPIIQAGMRHILSIVRHSLFVDKDLRRNIMY
                     SAIIGSATLSMAWFLQPYLQAELKMSAAHIGITWFIMNLAVGLATLIAYKVEKVAGAR
                     NTLILIALTIPGGYILMAYVHSWIVLGVVLLFYIVRGIATPVLKDYINRITTSDIRAT
                     VLSVRNFLIRILFSIIGPFVGYFADVYSLRQALYLAGTLFFVFSAITLLFFLKSLPRN
                     SIITKENSEAC"
     gene            3870..5660
                     /locus_tag="PLK75_07685"
     CDS             3870..5660
                     /locus_tag="PLK75_07685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOY39166.1"
                     /translation="MTRVLITTDDLLNAIQRKGWLWRLSSVAILHLMGLRKLNKAYNL
                     MYRPGAAEFIDAMFQCNNNSIAVESTQLENIPREGGFIVVSNHPYGSWDYVTLIKIFS
                     EIRPDSKFMANFLVSRIEPLKPFLFDNKQAIRRLKRKKSEAHDYKSILRHISLGHPAG
                     FFPAGEVSSFNERMKRVFDRKWQQSIVRLIYNSKVPVIPVFFPGSNSNWFHRFGKIHP
                     LLLTLTLPGEMLRLTNTQIEVNLGSPVNQSIKNIIKDENVYGSFLKMCCYALGNTVNY
                     KSKTTAFETPEPIAESVLQGLIKEEIESIKASCFLFCIENYHCYLASKSEIPNIFTEI
                     SRLREITFREIGEGTGKSCDTDKFDEYYKHLFIWDFTAEKLVGAYRIGFGKEIMKDFG
                     ADGFYTSTLFEFKPKFYPYMENGIELGRSFVVNEYQKKSSALFLLWKGIIYVLLKKPE
                     YRYILGPASISNNFSDIAKICMVEFFKRNFSDDDLSHYVSCRNEFRYKLPRDLNVVLK
                     YCGNDIKKIDRIIEEFSTNGMKIPVLLRKYLSMGAKIVHFNVDPEFNNAIDGFMINEV
                     KELPLSSVLAFAKEVNDPELIARFSGQEES"
     gene            complement(5638..6816)
                     /locus_tag="PLK75_07690"
     CDS             complement(5638..6816)
                     /locus_tag="PLK75_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOY39167.1"
                     /translation="MFDFDKPINRENTFSVKWDNRTEVFGTNDVYPLWVADMDFEAPK
                     EVTDAILKRAEHRVYGYSLHADAYFNAFISWSQRKHNWEVQREWCFFSPGIVPALAVA
                     VQTFTGEWDKVLVQSPVYNPFFDVVEGNKRELVHSPLILKNGRYHMDYESLEKEFAGG
                     VKLMLLCNPHNPGGRVWSKEELTRLAELSLKYEVVVFSDEIHCDLVYPNQKHTPYLSL
                     GEKYLPFSAVSQSPSKTFNIAGLSSSVVVIADKEMRDRMKNLMETIHLCMGNIFGTEA
                     FKTAYTLCDSWHSELISYLEMNLKLATSFIQNQLPVIKVMQPDAGFLLWLDFSDLGLS
                     DKMLNEFLVTKAGLGLSAGVMYGHEGSGFMRLNFGLNRAQLEKCLIRLQQAVNSLPVR
                     "
     gene            6877..7293
                     /locus_tag="PLK75_07695"
     CDS             6877..7293
                     /locus_tag="PLK75_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39168.1"
                     /translation="MKKYLSLISLVFVSIMLLTGCGQTDEDIANTAILIGHWRPVDSD
                     GNLVEDVPVYYFAEDNQGNSSLYNTTDTMQWELKRNQLKIYYEQSPGYYIGYDKYNSR
                     SLARLREISTNSFKVTQFYSDGFQREISFQRFDPEE"
     gene            7375..8697
                     /locus_tag="PLK75_07700"
     CDS             7375..8697
                     /locus_tag="PLK75_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY39169.1"
                     /translation="MSETIIPKSIVEEKIAAVGLKDLGKATIREIVGLVNQLQDATGV
                     KFLRMEMGEPGLLPSEIGTQAEIEALKRGVAASYPMVDGVKELKQEASRFVKNFMNIE
                     ISPAACISTVGSMQGGYATFLINNFLDPKKDTALFIDPGFPVQKQQYRVLGQKFVSFD
                     VYNYRGKKLREKLISFFEQGNINSIIYSNPNNPAWICLTDEELQIIGELCTKYDVIAV
                     EDLAYFAMDFRRDMSKPGEPPYQLSVANYTKNYVLLISSSKAFSYAGQRLAVTCISDA
                     IFHRCYPNLKIRFGTEAYGYTFVMRILYSLSSGASHSAQYALAAMFRAASDGKFNFVK
                     EVSEYGERAHRMKKLFVEAGFEIVYDKDIDQDLADGFYFTIQYPGKSGHEIIKELLCY
                     GISAISLENTGSEKINGLRACVSQIKLPMIDELEQRLKRFAANHRISN"
     gene            8715..9491
                     /locus_tag="PLK75_07705"
     CDS             8715..9491
                     /locus_tag="PLK75_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY39170.1"
                     /translation="MIQSSEVIRITEWLQGFAKQHHTGEASIDSNIDLKLNHSLRVAQ
                     EAKSIAKSLNLSDSDINLAQICGILHDVGRFRQYKIYQTFVDSQSVYHGTMGVDVLNM
                     VNVLDKFDKKTVNIIHAAVYNHGLIAIEEGLPERELLFSKITRDADKIDIYRIVTLYY
                     HQSDNRNSALELGLTREEIIDNAVLEDFATGKVVEKTDLKSLNDFKVLQLSWINDINY
                     GFTAKTIAERNFLNQIVNSIEGEENRNLIAKILKTKLDKM"
     gene            9503..10843
                     /locus_tag="PLK75_07710"
     CDS             9503..10843
                     /locus_tag="PLK75_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOY39171.1"
                     /translation="MKAFKAYDIRGEYNKDFTKQDAYKIGYFLPRLLPTDKILVGRDA
                     RLSSDEIFDALSRGICDAGADVYDAGLCSTPMIYYGTAHFNFAASVQITASHNPPEHN
                     GMKISSAKAMPVGYDTGLKHIEQWMNEEEVIASPYRGKIVKYNISDSYYGFLEKFKSD
                     ISSLNVCIDLSNGMAGLFARRLFGETPHYINETIDGRFPGHEPNPLEQENLVQLKSAV
                     LKHKADIGIIFDGDADRVMFVDEKGEFISPDLIIALLGHYFSDKGIKGTAIQDIRTSK
                     AVGEYLQKLGFNMYTWRVGRAFAAPKLKEIDGVFGGELAGHYYFRDFYFSDSGILACL
                     LVLKVFLKMHAAGTTLSEAISNISKYKSSGEINFKIAKKQQAMDAVKEAFFSESAPVA
                     FYDFDGYRLDYDNFWFNIRPSNTEPYLRFIAEAKTQKELDAVVSRVKEICMQFS"
     gene            complement(10859..11491)
                     /locus_tag="PLK75_07715"
     CDS             complement(10859..11491)
                     /locus_tag="PLK75_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOY39172.1"
                     /translation="MLIKIFWQGIIIGLSASIPLGPIGILSIQRTISKGRHAGFVSGL
                     GAAAADAMYAIIAGFSISFIIDLITQYQDIIKVVGGAFLLFIGVRLFITNPAKVMRKQ
                     MKQKNKGLWGDFLSSFGLTFSNPITLFVFIAVFAGFGLIDYQYKYVSILVLISSVFFG
                     ASLWWFSLTFLVSIFRNKIKMRRLLMINKVAAVLIFGFGLFVIVSLFFAK"
     gene            11574..12341
                     /locus_tag="PLK75_07720"
     CDS             11574..12341
                     /locus_tag="PLK75_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOY39173.1"
                     /translation="MNSGLEHLQEYLDYKAAYYNNSCFVEDDPVSIPHRFTNNSDIEI
                     SGLLTALISWGKRKSIIQSANKLMDMLDDSPYDFVKNHNSADLQRLKPWVYRTFNCTD
                     LCEMISALKTIYNHSQSLESLFINRSIYDGLAYLRTAILAQPHSKHLEKHISNVNAGA
                     AAKRLNMFLRWMVRSDNKGVDFGIWQNISPASLMMPLDVHTGRVARSLGLLKRKSDDW
                     KAVEELTTVLRQFDPDDPVKYDFALFGIGVYEGFRYI"
     gene            12345..13784
                     /locus_tag="PLK75_07725"
     CDS             12345..13784
                     /locus_tag="PLK75_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY39174.1"
                     /translation="MKAKGIIKQLPRNFWIANTLELFERWAWYGMFMVLALYLTGSTD
                     TGALGFSQEQKGILMGTVVAILYVLPVITGAIADKYGYKKVLAVAFTILSTGYGFMAW
                     FSNYTLVFMSFIYLAIGAGLFKPVISACIAKTTTPTNSSIGFGIFYMMVNIGAFIGPI
                     FASKLRGIDWYLVFVMSASVILLNLLLLIVFFKEPVRKKNTDALWKSITTIFKNIGTA
                     ITDFRFLIFLIIVIGFWTMYNQLFYTLPVFIEQWMDTTSVYQAIQNISPWLASKIGTE
                     QGSIAPEMLTNIDAMYIVLLQVLVSFAVMKLKPLHAMISGFLVSSIGIGLMFMFNNPM
                     YLFFSILIFGLGEMSGSPKISEYVGRIAPREKVALYMGCSFLPMAGGNFFAGLLSGTV
                     YQNMSDKITLLQHEVSARSLDIPAISDTFSQNDYIASACQQMHMNQTELTQYLWNTYH
                     PGNIWIVFSAIGLGTVILLVLYDRIFLKS"
     gene            complement(13926..14486)
                     /locus_tag="PLK75_07730"
     CDS             complement(13926..14486)
                     /locus_tag="PLK75_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOY39175.1"
                     /translation="MAQEKGKVEVYTDENIINRIYLVRGKKVMIDRELAELYGVETRV
                     LNQAVRRNAKRFPEDFMFQMTPEELEEWKSQIVISSKEKMGLRKPPLVFTEQGVAMLS
                     SVLNSDTAIMVNIQIIRIFTKMREMLETHKDILSKLDELERKGIERDEKIMLIFEYIK
                     QFEEAKQQELEYRNRKPIGYKRNEEN"
     gene            complement(14793..>16618)
                     /locus_tag="PLK75_07735"
     CDS             complement(14793..>16618)
                     /locus_tag="PLK75_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOY39176.1"
                     /translation="LPLYIEALENTEKSLGKEHTRYGASLKNLASFFYDMGQYHKALP
                     LYREAIQNNFYNINKAFSFLAESEKEKFIKTLSGNFNNYKSFFIDYAPENPEVARDAY
                     NIEIATKGMILQAGIQMRESIEQSGDSAALAKYDKWQALRLTIAKQHGQPVEKRLQNL
                     PEMEAEAEALEGELARVSSAFAESQKLGAITWQDVQHVLNPNEACVEFSSFSYQKDME
                     STDSIMYVALVLLPESEQPILVPLCEQKQLDSILYNTASTPGALNRMYRGASLPFKAG
                     VDYGERMYELLWEPIEDIIPENYTVYYAPSGSLHKIAFAAIPINESICLSQKYKLMQV
                     SSTTVLLQPSKFKTTKPESIALFGGINYETDMEELLATAENIRSKQEYVSRSISSDVE
                     RGSTAWGYLQGTLQEVNAIENIAKKQKVATTKFTGNTAIEEQFKSLKGNNSPRIVHIS
                     THGFFFEDAEQGEQYNAAKNPLNRAGLLFAGANNSWQGKASNDGIDDGILTAYEATNV
                     ILGNTELVVLSACETGLGEIRGSEGVFGLQRAFKNAGVEYIMMSLWKVPDAETAEFME
                     YFYNKLFGGESIPDAFRNTQQYMNKKYPDEPYKWAAFVLVR"
BASE COUNT         4835 a         3493 c         3347 g         4943 t
ORIGIN      
        1 acttgacagg acagtgcttc gaaatcggca acaaaaacat accgccaaac cgttaagctt
       61 cctccaaaaa cagcataaag tcatctgaaa ctaccgttat tcctttatga gtttgccact
      121 cgcattttcg cagccggtga agcggtaaaa atacaaacca ctctcccatt gctctgccgg
      181 aatccaggtg tctccttttg caattaccga ttgatagatt tgttttgcct ctgcattgta
      241 gctgctgata ataccctggt cgcaaccgga atgcactaag aacccgcttt gagcaggatt
      301 tggatgcacg gtaacgctgc tgttggcaat tatgccagaa cgaagcgcag tggtgtcgat
      361 gccaagtaca tatatttcgt cgaagtaaat accatcgtgg gttattgact gatcagaaac
      421 aaacttgatt ttaattttaa tcgactcccc gaggtaatcc tgcaaatcaa cctcgtcggt
      481 ctgccagata gttttaattc ccgtgtacac aggttgcccc tcgtcctgat cgtcggagcc
      541 aactcttgat agctttgtgc ataaaggttg ccagttttcg ccattgtcgg cagatacaaa
      601 tatttcgcaa taatcgtggg tgttttctat atcgaacctc actttaaaag taagtactgc
      661 aacactgata ttttcgagat taatgttatc gttagtaact atctccgaat tctggaacag
      721 ttcgtaattc ccatcaggcg attcggttat tgagcctgag cctgcgtaaa aaactgaatt
      781 ggaaatccca aaatcatcac cgctccattg ttccatggtt tcgcaatcgt cgtaaaacaa
      841 cgtagtaaca ggcccataag ttttggttac cgtatcgctg aatgtatata tgccattatt
      901 accggctact atataagaaa tcagatcgcc gtaggctgca tcaagagtgt acgcaatctg
      961 gccgcttctt ttttcaagca gagtcatatt gtcgaaaacc tgggtgttgt tctgaaagct
     1021 gatatttccc gacaaaggaa taaatgtaag ggtgaaattg gacggagtat caagtccaac
     1081 gcattccacg gtaaagtcaa gatttcctga caagtggtta atgatatcag gactgatgtc
     1141 atcgaacatg taaaacgcct gtgaaaagtg ggccatatta aggtttaaac gaactgcgcc
     1201 acggcaaagg tcatcgatgc gttcgatgct aggccagaaa ccatcgtcga gtgtaccaat
     1261 ttctgcagtc atcgaaagga ttttattttt cacccccact tcaccgtacg accagtcgtc
     1321 ggcatcgcca ttgctttgat atccgtaggt ttctccaaca tttccatacc tgtaatgatt
     1381 gtcttcggtc atcttttcgc atattgttaa aaacagcaat gaatcgggag tactctcatt
     1441 gatatagctc catggataaa ttagcatatt cccggcagca tgccagttta cttgaagttt
     1501 aatatcgtgt gcaataagaa actgactcat cgcaaacgac tcaggttccg aaaaagcatt
     1561 ttcgccccta taccatgggc tccacaccaa aggttgcgac ccgatgttgt cgtaagcata
     1621 ggcatatccg taattgcggt tcaggtcaac cccataatat gtctgaaaca tatagtcgac
     1681 agggcgtcgg tttttacgcc agtcgccacc tcccccgggg ttttcggtct cgttatatac
     1741 atacccgtca gggtttactg ccggcacaaa gtacatttcg gtattatcga tcagataagt
     1801 gatttctggg tcagtgccat aattttctaa tatatggaac ataaagaaaa tcaattgctg
     1861 aagcgagcag ggttcactgg aatgatgcaa agatgtataa agtacctgtt gctcatcttc
     1921 gcttatatcg ggattatccg atattttcac actataaatt ggtctgcctt cgattgaggt
     1981 tccaatctgt gtttttacac taatcaggtt cgggtacagg cttctcatct ggtcgagctc
     2041 agctatggtt tcatcgaagg tataataccc tcccatactt ccgtgagagt agccttcagg
     2101 cagcgggtag ttctgaactg tattaaaaca ctcgttaaaa acttcttctt tagctgcgtt
     2161 atttccccgg aactgatagt acttaacaag gtcttcaata atcacttcgt attgaagcgt
     2221 tgattcttca attttcatta catcgtaccc ttcgaaaaca ccggtaaatt ctccattctt
     2281 tttcagaaag ccatgaaccg gttcaactcc aatttcatgc agggcaagta ttcctttact
     2341 tccaagatca acgctcaaat acgactgagt gctttgcgct gtaatttgta acgaaagaac
     2401 agaaacaaac aaaagaacaa gtgagccaat tttaatcatg tcaacagttt tgtaaattat
     2461 ttgaagccaa agatatgaaa tgggcgaaaa caaacaacgt gcttcatctg tttttgttcg
     2521 ataaattaag caaaacaaaa gtaagaaata tggtattggc ttaaataaaa tcataaattt
     2581 gctgccttaa tctgccatga aaagcataaa ccgaaatatt tacatgctat acctgataaa
     2641 ggtagccaaa tggtttatgc tggtaatgcc gattattgtg cttttctacc aaagccatgg
     2701 attgagtctg cgcgatgtgc ttacggtaca ggctgtttat tcggtaagta tcgttttgtg
     2761 ggaaattcca tcgggatatg ctgctgatta ctggggtcgc cggaaaacga taattttggg
     2821 cagtattctg ggaactatag gttttgtaat ctatagcttt gcaaatggat tttggggttt
     2881 tatagctgcc gaatcggtgc taggcattgg tatcagtctc atcagcggag ctgactctgc
     2941 aattctgtac gacagtcttg ttgaaaaagg cagacaaaac gaatacatga aactcgaagg
     3001 ccgtgtgata tctatcggga acttttccga aacgctggca gctctggccg gtggtctgct
     3061 tgcagaaata agtctaagga caccttttat tgcccagact tttgtagcat ttattgctgt
     3121 tcctgcttcg tttctgcttt atgagcctgc cagaaccaaa cccattattc aggcagggat
     3181 gagacatatt ctatcaattg tcaggcactc acttttcgtc gataaagatc tgcggaggaa
     3241 tatcatgtat agtgctatta ttggttcagc tactttgagt atggcatggt ttcttcaacc
     3301 ctatttgcaa gctgagctta aaatgagtgc agcacatatt ggtataacct ggttcattat
     3361 gaatttggca gttggcctgg ctacccttat tgcttacaaa gtcgaaaaag tagccggagc
     3421 acgaaataca ttgattctta tagcattaac tattcccggt ggatatattc tcatggctta
     3481 tgttcattca tggattgttt tgggtgtcgt gctgcttttt tatatagtta gagggattgc
     3541 cacacccgta ctgaaagact acatcaatcg cattacgaca agcgacatca gagctactgt
     3601 tctttctgtg cggaattttt taatacggat acttttttcg ataatcggtc catttgttgg
     3661 gtatttcgcg gatgtgtatt cgttgaggca agcgttatat ctggctggta cgctgttctt
     3721 tgtttttagc gccattactt tgttgttctt cctaaaatca ttgccacgca atagcattat
     3781 aacaaaagaa aatagtgagg cctgttagtt tggcttattt gatgagattt ttttaatttg
     3841 catagcggtt tatatattta aaacaaccca tgacaagagt attaataaca accgatgact
     3901 tattaaatgc tatacagcgc aaaggatggc tgtggcggtt atcatcggtt gcaatattac
     3961 atctcatggg attgcgaaag ctgaacaaag cttataacct gatgtaccga cccggggcag
     4021 cagaatttat tgatgctatg tttcagtgca acaacaatag cattgctgtt gaatcaacac
     4081 agcttgagaa catcccccgc gaaggcggtt ttattgttgt atcaaatcac ccatatggaa
     4141 gctgggatta tgtaacatta ataaaaatct tttcagaaat ccgccccgat tccaaattta
     4201 tggcgaattt tctcgtcagc agaatagaac ctctgaaacc atttttattc gataataagc
     4261 aagccatccg caggttaaaa aggaaaaaat ctgaagccca cgactataaa tcaattcttc
     4321 gtcatatcag tctggggcac cctgccggct tctttcctgc aggagaagta tcatcattca
     4381 acgaaagaat gaaacgggtt ttcgatcgta aatggcagca aagcattgta cgactgatat
     4441 ataactccaa agttcctgtc atccctgttt tttttccggg ttctaacagc aactggtttc
     4501 accgatttgg aaaaattcac cccctgttac tcacattgac tcttcctggc gaaatgctcc
     4561 gcctaacaaa cacgcaaatt gaagtgaatc tgggctcacc tgtgaatcaa agcataaaaa
     4621 acataattaa ggatgaaaat gtttatggca gctttttgaa aatgtgctgt tatgccctgg
     4681 gaaataccgt gaactataaa agcaaaacaa ctgcattcga aacacctgaa ccaatagcag
     4741 aaagcgtttt acaaggttta attaaagaag aaattgaatc gataaaagca tcatgctttt
     4801 tattttgtat cgaaaattac cactgctatc ttgcgtcgaa aagcgaaatc ccaaacattt
     4861 ttactgaaat cagccgtttg cgggagatca cttttcgcga aattggcgaa ggtaccggaa
     4921 agagttgcga tactgataag ttcgatgagt actacaaaca tttgttcatt tgggatttta
     4981 ctgccgaaaa gcttgttgga gcataccgca tcgggtttgg taaagagata atgaaagact
     5041 ttggagcaga tgggttttat acatctacat tgtttgagtt caagccgaaa ttctatccgt
     5101 atatggagaa cggcatcgag ctcggaagat cgttcgtagt gaacgaatac cagaagaaat
     5161 catctgcgct gttcttgttg tggaaaggta ttatatatgt attattaaaa aaacctgagt
     5221 acaggtatat tcttggtccg gccagtatta gtaacaattt tagcgatatc gccaaaatat
     5281 gcatggtcga gttctttaag cgcaacttca gcgacgatga cctatcgcac tatgtatcat
     5341 gccgaaacga gttccggtat aaattaccca gagaccttaa tgtcgtactg aaatattgtg
     5401 gaaacgatat taagaaaatc gacaggatta ttgaggaatt tagtactaat ggcatgaaaa
     5461 taccagtgtt attgcgtaaa tatttgtcga tgggcgctaa aatcgttcat ttcaatgttg
     5521 accctgaatt caacaatgct atcgacggtt ttatgattaa cgaagtgaaa gagttgccat
     5581 tgagctcagt acttgctttc gcaaaagaag ttaatgaccc tgaactgatt gcaaggttta
     5641 gcggacagga agagagttga cagcctgctg caaacgaatc agacattttt caagctgagc
     5701 cctattcaat ccaaaattca atcgcataaa gccgctacct tcgtgcccat acataactcc
     5761 tgccgacaat cccaggccag ctttagtaac caggaactca ttcaacattt tatcgctgag
     5821 tccgaggtcg ctgaaatcga gccatagcaa aaaacctgca tcaggctgca ttacttttat
     5881 cacaggcagt tggttttgaa tgaacgatgt tgctaatttc aggttcatct ctaaatagct
     5941 gataagttca ctgtgccagc tatcgcatag cgtgtaagcc gttttaaatg cctcagtacc
     6001 aaaaatatta cccatacaaa gatgtatggt ttccataaga tttttcattc tgtcgcgcat
     6061 ttctttatcg gcaataacta caacagagct gcttaaaccg gcaatattaa aagttttact
     6121 aggcgattgg cttactgctg aaaaaggcag gtacttttcg ccaagtgaaa gataaggtgt
     6181 gtgtttttgg tttggatata ccagatcgca gtgtatttca tcggaaaaaa ctacaacttc
     6241 atatttcagc gataattcag caagccgtgt tagctcttct tttgaccaga ccctaccgcc
     6301 agggttatga ggattgcaaa gcagcatgag tttaactccc cctgcaaatt ctttctccag
     6361 cgattcgtaa tccatatgat aacgtccgtt tttaagtatc aaagggctgt gaaccagttc
     6421 tcttttattg ccctcgacta catcgaaaaa agggttatat acgggcgact gaaccagtac
     6481 tttatcccat tcacctgtaa atgtttgcac tgctactgcc agtgcaggga caataccagg
     6541 actgaaaaag caccattccc gctgcacttc ccagttgtgt tttcgttgcg accacgaaat
     6601 aaatgcattg aaataggcat cagcatgcaa tgaatagccg tatacccgat gttctgcgcg
     6661 ttttaatatt gcatctgtaa cttctttggg agcctcaaaa tccatatcgg caacccatag
     6721 cgggtacaca tcattggttc caaaaacctc agttctgttg tcccatttta ccgagaaggt
     6781 gttttcacgg ttgatcggtt tatcgaaatc aaacatatat tttataattt gcgcaaaaat
     6841 agtgtatttt tgaagttata attgttattg agaaaaatga aaaaatattt atcgctaata
     6901 tcacttgttt ttgtatctat aatgcttctt acaggctgtg gtcagaccga tgaagatatt
     6961 gccaatactg ctattttaat tggacattgg cgtccggttg acagcgacgg aaatctggtt
     7021 gaagatgtgc ctgtgtatta ctttgccgaa gataatcagg gcaactcgag cctttataac
     7081 actaccgaca ccatgcaatg ggagttgaag cgcaatcagc ttaaaatcta ctacgagcaa
     7141 tcgcccggat attatatagg ttatgacaaa tacaacagtc gcagccttgc ccgcctccgc
     7201 gaaatatcta caaattcttt caaagtaacg caattctaca gcgacggttt ccagcgtgaa
     7261 atcagctttc agcgtttcga ccctgaagaa taatgtttta taaccttctt tcgttgcaca
     7321 tctgagtttg tatttttcaa tgcaaataaa taagtttgta aaaactagaa tgttatgagt
     7381 gaaactataa ttccaaaatc gattgttgaa gaaaaaattg cagctgtagg ccttaaagac
     7441 cttggcaaag caactatccg cgaaatcgtg ggtctggtta atcaattaca ggatgccacc
     7501 ggtgttaaat ttctgcgcat ggaaatgggt gaacccggat tattaccatc cgaaataggc
     7561 acacaagccg aaatagaggc attaaagcgt ggtgttgctg cctcgtaccc tatggttgac
     7621 ggagtgaaag agcttaaaca agaggcttcg aggttcgtta aaaacttcat gaatatcgaa
     7681 atctctcctg ctgcctgcat ttcaacagta ggaagtatgc aaggcggcta tgctactttt
     7741 ctgattaaca actttctgga ccctaaaaaa gatacagcat tgtttatcga cccgggattt
     7801 ccggttcaga aacagcaata tcgcgtgctc ggtcagaagt ttgtcagttt tgatgtttac
     7861 aactatcggg gcaaaaagct tcgtgaaaaa ttgatctctt tcttcgaaca aggcaatatt
     7921 aactccatca tttattcaaa ccccaataac cctgcatgga tttgccttac cgatgaagag
     7981 ctgcagatta taggagaatt atgtaccaaa tacgatgtaa tagccgttga agatcttgca
     8041 tattttgcca tggatttcag gcgcgacatg tcgaaacccg gtgaaccacc atatcagctc
     8101 agtgttgcca actacacaaa aaactatgtg ctacttatct ccagctcaaa ggcattcagc
     8161 tatgcagggc aacgattggc ggtaacctgt atttctgatg ctattttcca ccgttgctac
     8221 cccaacctaa aaatccgttt cggaaccgaa gcatacggat atacttttgt tatgagaata
     8281 ctctattcac tgagttcagg ggccagtcat tcggcacagt atgctttggc agcaatgttt
     8341 cgtgcggcta gcgatgggaa atttaacttt gttaaggaag tgagcgaata tggcgagcgt
     8401 gcccatcgca tgaaaaagct ctttgttgaa gcaggttttg aaatcgttta cgacaaggat
     8461 attgatcagg acttggccga tggtttctat ttcacgatac aatacccggg aaaatcgggt
     8521 catgaaatca ttaaagagtt gctttgttac ggaatcagtg ccatatcact cgaaaacaca
     8581 ggcagcgaaa aaatcaacgg gttgagggca tgcgtttctc agataaaatt accgatgatt
     8641 gatgaacttg agcaacgttt aaaacgattt gctgcaaatc acaggatatc taactaacac
     8701 cgcattattt tgcaatgatc caaagcagcg aagttatacg aatcacagag tggttgcagg
     8761 gctttgcaaa acagcatcat accggtgagg cttctatcga tagcaatatt gaccttaaac
     8821 tgaatcatag ccttcgcgtt gctcaggaag ccaaaagcat tgccaaaagt ctgaatttgt
     8881 cggacagcga cattaatctt gcacaaatct gcggaatatt acacgatgtt ggacgattca
     8941 gacaatataa aatctatcag acatttgtcg actcgcaatc ggtgtatcat ggcacaatgg
     9001 gtgtagatgt tctcaatatg gtgaatgttc ttgataaatt cgataagaaa acagtaaaca
     9061 ttatccatgc tgcagtatat aatcatggat taatcgctat tgaagaagga cttcccgaaa
     9121 gagagctcct gttcagtaaa ataacccgcg atgctgacaa aatagatatt taccgaattg
     9181 taaccctgta ttatcatcag agcgacaacc ggaattcagc acttgaactg ggacttaccc
     9241 gcgaagaaat catcgacaat gctgtgctcg aagatttcgc gactgggaaa gttgttgaaa
     9301 aaaccgatct gaaaagtctg aatgatttta aagtactgca acttagctgg ataaatgaca
     9361 tcaactatgg attcacagca aagactattg cagaaagaaa ctttctgaat cagattgtga
     9421 attccatcga aggtgaagag aaccgcaatc tgattgcgaa aattcttaaa acaaaactgg
     9481 ataaaatgta aaaacaattg atatgaaagc attcaaggct tatgatatca gaggcgaata
     9541 caacaaagat tttactaaac aggatgcata taaaatcgga tattttctgc cgcgcctttt
     9601 acctaccgat aaaatacttg taggcagaga tgcacggctc agctctgatg aaatttttga
     9661 tgcactatct cggggaatat gtgatgccgg cgccgatgtt tacgatgccg gactctgctc
     9721 aactcccatg atatattatg gtacagccca tttcaatttt gcagcatcgg ttcagattac
     9781 tgcctcccac aaccctcccg agcataacgg aatgaagata tcatcagcca aagccatgcc
     9841 tgttgggtat gatacgggat tgaagcacat tgaacaatgg atgaatgaag aagaagtgat
     9901 tgcttctcca tatcgtggta aaattgtgaa atacaacatt tcagatagtt attatggttt
     9961 tcttgaaaaa ttcaaatcag atatttcttc gctgaacgtt tgcatcgacc tttccaacgg
    10021 gatggcagga ttatttgcac gacggctttt cggagagaca ccccattaca ttaatgaaac
    10081 gatcgacggc agattcccgg gacacgaacc caacccactc gaacaggaaa atctggttca
    10141 gctgaaaagt gccgttctca aacataaggc tgacatagga attattttcg atggcgatgc
    10201 cgatagagtg atgttcgttg atgaaaaagg agaattcatt tcgcctgacc tcataatagc
    10261 tttgctgggg cattatttct cagacaaggg cattaaaggg actgctatcc aggatattcg
    10321 aacaagcaaa gccgttggtg aatacctgca aaagctaggc ttcaatatgt acacatggcg
    10381 cgttgggcgt gcattcgctg cacccaagct gaaagaaatt gacggggttt tcggaggtga
    10441 gctggccgga cattactatt ttcgcgattt ttacttcagc gactccggca tactcgcatg
    10501 cctgctggtt ctaaaagttt ttctcaaaat gcatgctgcg ggaaccacac tttcagaagc
    10561 aatcagtaat atatcaaagt acaaatcttc aggcgaaatc aattttaaaa ttgcaaaaaa
    10621 acaacaagcc atggatgctg taaaagaggc ttttttcagc gaatcggcac ctgttgcttt
    10681 ttacgatttc gacggatatc gcctcgacta cgataacttc tggttcaata tccgcccatc
    10741 aaacaccgaa ccctatctga gatttattgc tgaagccaaa acccagaaag aactcgacgc
    10801 agttgtcagc cgcgtaaaag agatatgcat gcagttttca taaaagaact gatattcctt
    10861 atttcgcgaa aaataaactc acaataacaa acaagccaaa accaaagatc agcactgctg
    10921 ctaccttgtt tatcatcagc aatcgcctca ttttaatttt attcctgaaa atacttacaa
    10981 gaaaagtcag cgaaaaccac catagtgagg ctccaaagaa aacactggat ataagtacaa
    11041 gaattgacac atatttatac tgataatcaa tcagtccgaa tccggcgaaa acagcaataa
    11101 acacaaaaag agtaatggga ttcgaaaaag taagtccaaa cgatgacaga aaatctcccc
    11161 atagcccctt attcttctgt ttcatttgtt ttcgcatcac ttttgctgga ttggtgataa
    11221 acaatctaac cccgataaaa agaaggaaag ctccaccaac caccttaatg atatcctgat
    11281 attgggtaat taaatcgata atgaacgaaa tgctgaatcc ggcaataatt gcgtacattg
    11341 catcggcagc ggctgctccc aatccgctca caaaaccggc gtgtctgcct ttggaaatgg
    11401 ttctctgaat gctcagtatt ccaatgggtc ccaaaggtat cgaggctgat aacccaatga
    11461 ttattccctg ccaaaaaatt ttaattaaca taagctcatt gcatgaattg caaaataatg
    11521 aaagatttta cttttttaaa actattttcg tataaagcta ttattattgt tacatgaatt
    11581 caggactgga acatttacaa gagtatctcg attataaggc agcttactac aataactcat
    11641 gttttgttga agacgacccc gtgagtattc cccaccggtt tacaaacaac agtgatatag
    11701 agatttcggg gcttttaact gcactgattt catggggaaa aagaaaatca ataattcaaa
    11761 gtgcaaataa acttatggat atgctcgatg actctccata cgattttgtt aaaaatcaca
    11821 attcagccga cctgcaaaga ctgaaaccct gggtatatcg tactttcaac tgtacagacc
    11881 tgtgtgaaat gatttcagca ttaaagacaa tatacaacca ttctcaaagc ctcgagtcat
    11941 tgttcatcaa cagaagtatc tatgatggcc ttgcttactt gcgtaccgct attcttgcac
    12001 aaccgcacag taagcatctc gaaaaacaca tcagcaacgt aaatgccggt gccgcagcaa
    12061 aaaggctgaa catgttcctt cgatggatgg ttcggagcga caacaaggga gtggattttg
    12121 gaatatggca gaacatctcc cctgcatcac tcatgatgcc ccttgatgtg catacaggca
    12181 gggttgcgcg ttcgctcgga ctgttgaaac gcaaatcgga cgactggaaa gctgtggaag
    12241 aacttacgac tgtgcttcgg caattcgacc ccgatgaccc tgtgaaatac gattttgcac
    12301 ttttcgggat aggtgtttac gaaggttttc gatatatttg agacatgaaa gccaaaggca
    12361 taataaaaca acttccccgc aatttctgga ttgccaatac acttgaacta ttcgagcgct
    12421 gggcatggta tggcatgttc atggtgctgg ctctctatct tacgggtagc accgatacag
    12481 gggccctggg gttcagtcag gagcaaaaag gtattttaat gggaaccgtg gtggctattc
    12541 tctatgtttt acctgtcatt acgggcgcaa ttgccgataa atacggatat aaaaaagtac
    12601 ttgcggttgc gtttaccata ttatcgaccg gttatggttt tatggcatgg ttcagcaatt
    12661 acacgctggt atttatgagt tttatttatc ttgccatcgg tgccgggctt ttcaaaccgg
    12721 taatatcggc atgtatagcc aaaacaacca caccaacaaa ttcttctatc gggttcggaa
    12781 tattttatat gatggtgaac atcggggctt ttataggacc catttttgct tcaaaattac
    12841 gcggtatcga ctggtatctg gtttttgtga tgtcggcatc ggttattctg ctaaacctgt
    12901 tgctgctaat tgtatttttc aaagaacccg taagaaaaaa aaacactgat gctttgtgga
    12961 aatcgattac cactattttc aaaaatattg gcactgccat caccgacttt cgttttctga
    13021 tttttctgat cattgttatc ggtttttgga caatgtataa ccaattgttt tacaccctgc
    13081 cggtgtttat tgaacaatgg atggatacaa ctagcgtata tcaggcaatt caaaacatca
    13141 gtccatggct ggcttcaaaa attggaactg agcaaggaag cattgccccc gaaatgctta
    13201 ccaatatcga cgcaatgtac atagtgttgc ttcaggtatt ggtttcgttt gcggtaatga
    13261 aattaaaacc actgcacgcc atgatatcgg gttttctggt gagcagcata ggcatcgggc
    13321 ttatgttcat gttcaacaac ccaatgtatt tgtttttcag cattttgatt tttggtctgg
    13381 gcgaaatgtc ggggtcacca aaaatctcgg aatatgtcgg acgcatagct ccccgcgaaa
    13441 aagtggctct gtatatgggt tgcagctttc tgcccatggc aggaggcaat ttctttgccg
    13501 gtttgttgtc cggtacggta tatcagaata tgtccgacaa aatcaccctg ctgcaacacg
    13561 aagtatcggc ccgttctctc gacattcctg ccatcagcga cacattttcg caaaacgact
    13621 acattgccag tgcgtgccaa cagatgcaca tgaaccaaac tgaactaacc caataccttt
    13681 ggaacaccta ccatccgggt aacatctgga tagttttcag cgccatcggt ctggggacag
    13741 taatactgct ggttttatac gacaggatat ttctcaaatc gtagatttgc aacgctttac
    13801 tttctgggtt tcaaaaactt ctgcaaccag ttgaaaatct gtttattaaa acaatatcag
    13861 ctctcgtatt gcaaatgctt attgtaaatg catcctcgtt gcaaacgagg gcgggagaac
    13921 gttttttaat tttcttcgtt tcgcttatat ccaataggtt tacggttgcg atattcaagc
    13981 tcttgttgct tagcttcttc aaattgtttt atatactcaa aaattaacat gattttttcg
    14041 tctctttcga tgcctttccg ttcaagctca tctaattttg aaagtatatc tttatgcgtt
    14101 tcaagcattt cacgcatctt tgtaaaaatt ctgattatct ggatgtttac cattatggca
    14161 gtatcactat ttaaaacact tgacaacatg gcaactcctt gttctgtaaa aactaacggg
    14221 ggtttcctaa gacccatctt ttccttactg gatatcacaa tttgtgattt ccattcttct
    14281 aattcttcag gtgtcatctg gaacataaaa tcttcaggaa aacgttttgc atttcgtctt
    14341 actgcctgat tcaatactcg tgtttccact ccatacaatt ctgccaattc acggtctatc
    14401 attacctttt tccctctgac aagatatatg cgattgatga tattttcatc ggtatagact
    14461 tctacttttc ctttttcctg tgccattttt tcaatttcat attattacga aagtactatt
    14521 taaatttgga atcacttatt aaatacacca ttctttaagg tctcaatttg ctaccttaaa
    14581 aattattgta acggatgcag tttttgtata gctgatttta attcttctat tccactttat
    14641 ctatatgcac tgcgatcatt gcattgcaaa tgcttattgt aaatgcgtcc tcgttgtaaa
    14701 cgaggacggt tcagggctat taaagcaaac atagataaac gccttctctt ccccgaatca
    14761 attcatcctc acatcaaatt tatcattcaa catcacctca ccagcacaaa cgctgcccat
    14821 ttgtatggct cgtcggggta ctttttgttc atgtattgct gtgtattcct aaaagcatcc
    14881 ggtatggact caccaccaaa cagtttattg taaaagtact ccataaactc agcagtttcg
    14941 gcatcgggaa ctttccacag actcatcata atgtattcga ctcctgcatt tttaaatgct
    15001 ctttgtaagc cgaacacacc ttcgctcccg cgaatttcgc ccagaccggt ttcgcaggcc
    15061 gatagtacga ccagttcggt gttccccagt ataacattgg tagcttcgta agcagtgaga
    15121 atgccatcgt cgatgccatc gtttgatgcc ttgccctgcc atgaattatt ggctccggca
    15181 aacagcagtc cggcacggtt cagcgggttt ttagctgcat tgtactgctc cccctgctcg
    15241 gcatcttcaa agaaaaagcc gtgggtagat atgtgaacga ttcgtggtga attgttaccc
    15301 tttaggcttt taaactgttc ttcgatggct gtattgccgg taaatttggt tgttgctact
    15361 ttttgctttt tcgcaatatt ctctatcgca ttcacttcct gcaaggttcc ctgcaaatat
    15421 ccccaagcgg tgcttcccct ttcaacatct gatgaaatcg aacgcgacac atactcctgt
    15481 ttgcttcgta tgttttcggc tgttgccagt aattcttcca tatccgtttc gtaattaata
    15541 ccaccaaaca atgcaatgct ttcgggtttt gtggttttga acttcgacgg ctgtaacagc
    15601 acagtagtac tgctaacctg catcaatttg tatttctggc tgagacagat actttcgttt
    15661 ataggtatgg ctgcaaatgc aattttgtgc aagctaccgg atggagcata atacaccgta
    15721 tagttttccg ggataatatc ttcgataggt tcccatagca attcatacat tctttcacca
    15781 taatctactc cagccttaaa cggtagagaa gctccacgat acattctgtt taaagctccc
    15841 ggtgtagatg cggtattgta caaaatacta tctaattgtt tttgctcaca taaaggcacc
    15901 aaaataggct gttcgctttc aggcaataat accaaagcca cgtacattat gctatcggta
    15961 gattccatat ctttttggta tgaaaacgaa gaaaactcca cacaagcctc gttgggattc
    16021 agcacatgtt gcacatcctg ccacgtaatt gctcccagtt tttgagattc tgcaaatgct
    16081 gacgaaaccc gtgctaattc tccttccagg gcttcggctt cagcttccat ttcaggtaaa
    16141 ttttgcaatc gtttttcaac aggctggccg tgttgcttcg caatagttaa gcgcaaagcc
    16201 tgccatttat cgtatttggc aagagcagca ctgtcgccgc tttgctcaat ggattcgcgc
    16261 atttgtatgc cggcttgcag tatcatgcct ttggtagcga tttctatgtt gtaggcatcc
    16321 ctggctactt ccggattttc gggagcataa tcgataaaga agcttttgta attattgaaa
    16381 ttaccggaaa gtgtttttat aaacttctct ttttcgcttt cggcaagaaa actgaaagcc
    16441 ttgttgatgt tataaaagtt gttttggatg gcttctctgt aaagtggcag agctttgtgg
    16501 tattgtccca tatcgtaaaa aaaggacgcc aaatttttta atgaagctcc atatcgtgta
    16561 tgctctttgc ccaaagattt ttcagtgttt tctaatgcct caatataaag cggaaggg
//
LOCUS       DAOOQP010000063        16594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4241_length_16594_cov_5.558014, whole genome shotgun sequence.
ACCESSION   DAOOQP010000063 DAOOQP010000000
VERSION     DAOOQP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4241_length_16594_cov_5.558014"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLK75_07740"
     CDS             complement(<1..248)
                     /locus_tag="PLK75_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39177.1"
                     /translation="MKKALIICLIALATINVFSQGKSALFIGNSYTYYNDGVGYWVQK
                     IANSLGDEFEYQMVAPGGYQFNQHCVYTPTLDAIALGS"
     gene            complement(546..1427)
                     /locus_tag="PLK75_07745"
     CDS             complement(546..1427)
                     /locus_tag="PLK75_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOY39178.1"
                     /translation="MKQKILILCLPLLLGFLSVSAQEKAPYRIYDSKGKASTYNEMLS
                     TTSEADVVLFGEQHNNAVAHWFEIELAKDLFQTKGNNLMLGAEMFEADEQLILDEFVN
                     GTITEKNFLEQARLWPNYATDYAPLVNFARNSKIRFVATNIPRRYASMVSKGGFEVLG
                     DLSEEAKKYIAPLPVEYDSELSCYKSLLTMGGMGMGQGNANPNFPKAQAIKDATMAYF
                     IIKNLQPETIFLHYNGAYHSDNYESIVWYLNKSDKKLKIITISTVEQSDINVLSNDYK
                     GLADFIIVVDEDFTKTY"
     gene            complement(1663..4602)
                     /gene="dnaE"
                     /locus_tag="PLK75_07750"
     CDS             complement(1663..4602)
                     /gene="dnaE"
                     /locus_tag="PLK75_07750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOY39179.1"
                     /translation="MYLNCHSYYSLRYGALSLSDLIDGALRNDMDTIALTDINNTTAL
                     PEFVFLAQSKGLKPVGGADIRKGNRRIAVCIARNNPGLREINSFLSDIAINGRKHDAI
                     PDFDNAYVIFPAGCVPDRPLKDNEYVGVALWQTHLLPLLVAKYRITKMLVLHSLSVGS
                     DESYELHRHLRAIDKNTLLSKLLPADLAQWGDRFVSPSTIRTAFAAYPEVVSNTLNLL
                     HNCSLHFDSSVKNLKMFTGNARDDRNLLAREAYAGLVLRYGTDNAEAMRRLEHELGII
                     ENMGFASYFLIAWDLVRYTMSRGFYHVGRGSGANSIAAYCLRITDVDPIELDLYFERF
                     LNPKRSSPPDFDIDYSWSDRDEVHNYLFDRYGKERCALLGAMSTFRSRAALRELGKVY
                     GLTGDELENLSHRPAQYGRSDDVHRRIVKMAAQMDGFPNLRTIHAGGVLISDEPITTF
                     AALDMPPKGYQTTQWDMYTAEHIGFEKFDILSQRGIGHIRESCEIILKNKGIEVDIHD
                     IPRFKQDEASRQLLKSAHTTGCFYIESPAMKGLLRKLRCEDYITLVAASSIIRPGVSS
                     SGMMQEYIRRFHNQDGFEYLHPVMKEQLEETYGVMVYQEDVLKVCHHYAGLDLADADV
                     LRRAMSGKFRSRAEFERIFDRFFEGCRSKGRPEEISREVWRQIASFAGYSFSKAHSAS
                     YAAESFQSLYLKAHYPLEFMTAVINNFGGYYHSWVYFQEARRLGANICLPCVNRSNYM
                     TCIRGSDIYVGFIHLQNLEQQLAHRIVVERERGGDYTGLGDFVKRIGTGREQLTILIR
                     SGAFSFTGKTKAELLWLAHDMTQKTKTPPPMPVTMFEPGLREWRLPAFNSDRLEDAYD
                     EIELIGFPVSMSWFDLLQTSFRGEIMAGDMLNHAGKTVRMLGLFVTLKYVYTSKKEIM
                     NFGAWLDAEGNFFDSVHFPASLKEYPFRGNGLYLMLGKVSEEFGHPTLDVQKLARLPL
                     KPDPREK"
     gene            5401..6417
                     /locus_tag="PLK75_07755"
     CDS             5401..6417
                     /locus_tag="PLK75_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39180.1"
                     /translation="MKKILLFTSLTTISLFTFSQLVTISGVNQIPQVGDTIHYTNINT
                     FGFELAGTGTVMDKTWDLSFMTEDDNLYFFYIDPAETPDAASFPTSNLAEGTDQAEGY
                     FYFKTGDYYMARKGVTGEMYIDYANDSATTFTFPITAGHDYDCTYEGMLYASGLEMGI
                     ANGDIHIEADAQGTLITPNGSTFTNVLRLHIVESFDGMYDVGTGTPMVVLSVEDDYYY
                     WFHEDYTSPILIYGVTTTESLGSNPEETEVLRYQPINNSSVVQNNSTKEMHVFPNPSS
                     GLVFITNSSHYTTFNIFDLNGKLLKTINASLTIDLSAYNNGIYFIEMIGATRCDYQRI
                     VITR"
     gene            6619..7527
                     /locus_tag="PLK75_07760"
     CDS             6619..7527
                     /locus_tag="PLK75_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOY39181.1"
                     /translation="MSKKIPEAFKEYLHTLQAKPGKPVSLIDDFKTCCHEKPVPEYDI
                     KAMLKKSISQLSTMQDKLYAHDKYGVLIVLQAMDAAGKDGAIKHVMKGLNPQGVKVWS
                     FKSPNSIELDHDYFWRHYSALPARGEIGIFNRSHYENVLVTRVHPEYVMNEKLPGIEK
                     VDDITPEFWQSRYEQINRFEKNLHENGTIVLKFFLHISKEEQRKRFLERIDNPSKNWK
                     LSPADSKERKLWDNYMHAYEEMISATTSGIAPWYIIPADDKWFARFCIASVIVAATKK
                     LDLKYPEVDDKKRAELLKIKEDLLNE"
     gene            7651..8400
                     /locus_tag="PLK75_07765"
     CDS             7651..8400
                     /locus_tag="PLK75_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY39182.1"
                     /translation="MLLAFISDIHEDVRMLEKALRTAHKLGAERILCLGDITGYSPIN
                     QNFASEKNAAACIDLLREHCEISIAGNHDMFTARRMSEFAAGIHYPKNWYDLPFTEKQ
                     RLTGNKVWLYEHEIDPLLGPDHLEWLRTLPELAYIDACELKICLTHYIYPDVSGSLQM
                     GHEPNSDAYVHRNRFSCDLYLSGHLHPNGCIVQGKKTAHRWVNIDFGEKYSVENGDYL
                     FAPACLRHPVNCGILLLDAATKSVQTVQIKS"
     gene            8501..10636
                     /locus_tag="PLK75_07770"
     CDS             8501..10636
                     /locus_tag="PLK75_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOY39183.1"
                     /translation="MQKFLENIAHEFQTPFQNYVLVFAVMLFIILLIPLFLRKIRIPG
                     IIGLIISGILIGPHGFNIIEKNSAVDLFSTIGLLYIMFIVGLELDLAKFIKNKYKSFV
                     FGFFTFAIPIAIGFPVCFYLLHYPLNTSILVATMFATHTLIAYPIVSKLGIAKNEAVA
                     ITVGGTIFTDTAVLVALAIITGSDTGFANTEFLIRLGIAFVVYILFVIFVVYRVSRWF
                     FKHLEEERNSQFIFVLSVVFLCAFLAEIAGLEPIIGAFTAGFTLNRLIPQTSSLMNRL
                     EFVGNSLFIPFFLIGVGMIVDPGVFLSGPETLIVAGILTVVALIGKWLAALLTQWTLR
                     YNSLQRKLIFGLSSSHAAAILAVTMVGHRMGIIDDTILNGTIILILITCIVASMVTEN
                     VGKKIVVTTASETDPETPVATEKILVPVSNPETMPRLIDLALMLKTTKKQVPVYGLTV
                     VEDDETAREKLRQSRKVLTEGVSYAAASEQHFEVIATIDQNVVSGIKRVAREISATDI
                     VLGSSGKTHISELFFGKTVTQIVSSTDQAIYVYNAAMPLNVHKHIHLACPRYSEKEPG
                     FFDWLEKVVRIATLLNLKTDVYSDNDTCKAIEKFIKYKKMAANFKYRDFDDWEDFLVL
                     SRYFGQEDLLIVVTPRKPGVSHSNVLDGIPAKLARHFAEYSFLVVYPAVNNNEEFRKY
                     SENYDASLIEKGIRSVKKSAEELGKLFKT"
     gene            10743..11264
                     /locus_tag="PLK75_07775"
     CDS             10743..11264
                     /locus_tag="PLK75_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOY39184.1"
                     /translation="MTNTFEKTTWESLNDNAIRMIGKDWMLIGAGTKEQGFNMMTASW
                     GGMGWLWEKPVSFIFVRPQRYTYQFTERENYYTVCFFSEEHREALKICGSKSGRDCDK
                     VKESGLTPIFTPSGCVAFEEAVIILECKKLYAGKIEESNFVDAEVVKSKYPNKDFHKM
                     YVGEIVNVWKKKQ"
     gene            11279..14158
                     /locus_tag="PLK75_07780"
     CDS             11279..14158
                     /locus_tag="PLK75_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY39185.1"
                     /translation="MKKLLFLSLLGLAFLSLQAQNFDYKPVAVDVYQLDNGLTVILSE
                     DHTQPQVFGGVIVRAGGKDDPPDATGMAHYQEHMLFKGTQELGTTNWEAEKIHIDSIF
                     MLYDELGKTTDEVTRANIQKKINEQSLKANEYVIPNELSNLIKVMGGTDLNAGTGQDI
                     TIYYNAFPPSEINKWLDLYSHRFENPVFRSFQAELEVVYEEKNMYSDIFIFPLLESFN
                     KQFFKNHPYGQQTLIGSVEDLKNPSLSKMFEFFKTWYVPNNMALIIVGDFDKEAIKPM
                     INEKFGKWVKRELPIRPVYEEKAFNGREFAEVKLSPIKLALLGFRTVPAGHSDEIVLE
                     VCNAILSNQNQTGLLDKLVNNNELMAAQALNMPYNDHGACLFLIVPKILGQKLSDAEQ
                     LVMNEIDKLKSGDFDDYMIENIKLELYRNYLLSMESNDYKANMLAAAFGRYQSVEDLL
                     SYPQKLMAITKDDVIKIAQKYYGDNYLAFYSNMGFPKKEKIEKPGFKPIVSNTESESQ
                     YARHFNQIAPVEKEITYINFENDITHVEISSNIDIYGVKNPANDIFSLKIKIKAGYEK
                     IPMLKYATDLMNYAHPEGVLFDDFKEKVARLGCTYSFSGNESYTEIEIIGVESNISDA
                     IQLVGELLNKPVIDQDKINILYEGEKANRKMERSEPDAVADALFEFVKYGKASDFIDR
                     LSLKEIKALQADSLAIAFRDACNYGVEFHYVGQNDIQTIADLCKTTFRFEASKFTETP
                     VVKPIAKFSENTIYFVHKKKATQSKVYYLANGFEYTPDIQASMDAFNLYFGGDFSGLV
                     LQEIREYRSMAYSAGAKYVAAIRKGEQTYFMGYVGTQADKTVEAMVVFDSLIRTMPEK
                     PERLKMIQQYLLLSSSSDRPHFRSLSEQILAWKIQGFESDPAKMKKECYTNLRFTDIS
                     GFYKEHLAGKPLAICIVGDKSKIDMAELSKYGKVVFIKESSLFNE"
     gene            complement(14167..15372)
                     /locus_tag="PLK75_07785"
     CDS             complement(14167..15372)
                     /locus_tag="PLK75_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOY39186.1"
                     /translation="MMIDTIKQLSSSILAETISFQEYIHCNPELSFCEENTAMYVNSI
                     LNRNGITTYLNYAPNSVVAVVEGCLPSAKKPVCVALRADMDALPITESLNHAPRSEKE
                     GIMHACGHDIHTASLLGTALILKKLENNFSGKVVFIFQPAEEKLPGGAKTMIDNGLLR
                     DFAPMLILGQHVLPGLPVGSFGFCKGEYMASTDEIYIRFSGRGGHAATPDITSNTVMA
                     LAEFVMSTQTIKQKSAKTSKPFVLSFGRLNADGATNIIPNEARAEGTFRCFDELFRAE
                     VKRKLHILANEIAPRYKCEAHLKIVDGYPFVLNNPEFTDYCMQIAADFSGADRVVRLE
                     PRLTAEDFGYFSHEIPAVFYRMGIAADGKGVTNLHNPDFDHSPAALKDAPAMMAWLAM
                     KLVNDFPAK"
     gene            complement(15369..15737)
                     /locus_tag="PLK75_07790"
     CDS             complement(15369..15737)
                     /locus_tag="PLK75_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY39187.1"
                     /translation="MDFNTVILLVLAGLVAGVLGGFMGVGGGIVVIPVLVYVLGTSQH
                     TAQGTSLAFMLAPVGIFAFINYYKAGYVNVKYAIIIAVAFMVGSYFSSKWAVQMNTDT
                     LKKVFGALIILVGIKMLLGK"
     gene            complement(15743..>16594)
                     /locus_tag="PLK75_07795"
     CDS             complement(15743..>16594)
                     /locus_tag="PLK75_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39188.1"
                     /translation="FNQVSSVQLTLSGRHDKMTDLSLDYFSLLSEPEYNYWAGTKLEY
                     IFDNTLEKQINIYNGFRYKVFAEYYRQIDKNQSDLIVFGGDFRYYLPLHRNLIIAARV
                     AASGSAGRSKLIYYLGGIDNWINFSTKTPTFDNTVRIDPDKNYVYQAVATNMRGFSQN
                     VRNGTNFAVLNAEIRWPLVSYILNRPLNSAFLQNLQLIGFFDMGCAWSGLNPFSGDNA
                     FDNDVYENYPVTVIIDNNNYPIVAGYGVGVRSKLFGYFIRADWAWGIENNRRLPGIFY
                     LSLNLDF"
BASE COUNT         4919 a         3666 c         3531 g         4478 t
ORIGIN      
        1 cacgatccaa gggctattgc atccaaagtc ggggtatata cacaatgttg gttaaattga
       61 taaccaccgg gagcaaccat ctggtactca aattcatctc cgagagaatt cgcaattttt
      121 tgcacccagt aacccacccc atcgttgtaa tatgtataac tattgccaat aaagagagcc
      181 gatttaccct gcgaaaacac atttattgta gccaaggcta tcaaacaaat aattagtgct
      241 tttttcataa ttcagaatta aatatgtaca agacgaagta ttttataaag ggttgcgaat
      301 actaccagta acgtattatt aaattggaga agacagaatg tagaatacag gagatagaat
      361 acagaaggga gcggtagtta attcgaaggc atttgcctct ggcttgatgt tgccaaaatc
      421 tcttggagca gtaggtttcc ctcgcccaac gaaacgtacc cttccgtatt cttaattctg
      481 tctcctgtat tctgctttaa aacacctcgt catttataat gatttcatca ccctatattt
      541 acaccttagt atgtctttgt aaaatcctca tcaacaacaa tgataaaatc agcaaggcct
      601 ttgtagtcgt tgctaagcac attgatatcg gattgttcta ccgttgatat ggtaataatc
      661 ttcagctttt tatctgattt gttgagatac cagacaatgc tttcgtagtt atcggaatga
      721 tatgcaccat tatagtgaag aaatatcgtt tctggttgca ggtttttaat aatgaaatac
      781 gccatggtag catctttaat ggcttgtgct ttgggaaaat tcgggtttgc attgccttga
      841 cccattccca ttcctcccat tgtcaataag cttttgtaac agctcagttc actatcatat
      901 tccaccggaa gtggtgcaat atattttttt gcttcttcgc tcaaatcacc gagaacttca
      961 aaaccccctt tggaaaccat tgatgcatag cggcgaggga tattcgtggc aacaaaacga
     1021 atcttgctat tgcgtgcaaa atttacaagc ggagcatagt cggtggcata attgggccat
     1081 aatcttgcct gttctagaaa gtttttttct gtaattgtgc cattcacaaa ctcatctaaa
     1141 atcagttgtt cgtcagcctc aaacatttcg gcgcccagca tgaggttgtt gcctttcgtc
     1201 tgaaacaaat cctttgccag ctcgatttca aaccaatggg caacagcgtt gttgtgctgc
     1261 tctccaaaca acactacatc agcttcggag gttgttgaca acatttcgtt gtaagtactt
     1321 gcttttcctt tagaatcgta aatacgatat ggcgcttttt cctgggctga taccgataaa
     1381 aatcccagta gcaagggcag gcaaagaatc agtattttct gtttcatatt ttttttagta
     1441 aaaatagaaa aatgaaatat ataatacaac tgacaacagt taaattcgat aatcggaatg
     1501 gcggccgcct agcgaagtcg aggcgcccgc catttgaaaa caatattttt tatctcggta
     1561 acatttcgcc gacaactcga ctacgatgtc cggctcaaag actggcttgt tgacatccat
     1621 tggggcatat tgaaaacctg agtttgtcag agcaacgttt gtttacttct ctctcggatc
     1681 aggtttcaac ggcaatctag ccagtttctg cacatcgagg gtgggatgcc cgaattcttc
     1741 gctcacttta cccagcatca gatagagacc attacccctg aacggatatt ccttcaggct
     1801 ggccgggaaa tgcaccgagt cgaagaaatt gccttcggca tcgagccagg caccaaaatt
     1861 cataatttct ttttttgagg tgtacacata cttcaacgta acaaacaagc ccagcatacg
     1921 gacggttttt ccggcatggt tcagcatatc gccggccatt atctcaccgc ggaatgaggt
     1981 ctgcaacagg tcgaaccaac tcatactaac gggaaagcct atcagttcaa tttcgtcgta
     2041 ggcatcttcg agcctgtcgg agttaaaagc gggtaggcgc cattctcgca atccgggttc
     2101 gaacatggtt accggcatcg gaggcggtgt ttttgttttc tgcgtcatat cgtgggccag
     2161 ccacaacagc tcagccttgg tttttccggt aaacgaaaaa gcaccgctgc gtatcagaat
     2221 ggtcagttgt tctcttcccg taccaatgcg ttttacaaaa tcgcccaatc ccgtataatc
     2281 gcctccccgc tcccgttcaa ccacaattcg gtgcgccagt tgttgctcaa gattctgcag
     2341 gtgaatgaac cccacgtaaa tatcgctgcc gcggatgcag gtcatataat tgctccggtt
     2401 cacgcatggc agacaaatat ttgcccccag gcgacgggct tcctgaaaat agacccacga
     2461 atgatagtat ccgccaaagt tattgatgac tgccgtcata aactcaagcg ggtaatgcgc
     2521 cttcagatag aggctctgaa aactctcggc cgcatacgac gccgaatggg ctttcgaaaa
     2581 ggaatatccg gcaaacgagg caatctgccg ccacacctca cggctgattt cttccggacg
     2641 acccttgctg cggcagcctt cgaaaaagcg atcgaaaata cgttcgaact ccgcccgcga
     2701 tctaaattta ccgctcatgg cacgacgcag cacatcggca tcggcaaggt cgagccctgc
     2761 ataatgatga cacactttca gcacatcttc ctgatacacc atcaccccat aggtttcttc
     2821 aagctgctct ttcatcaccg gatgcaagta ctcaaaacca tcctgattat ggaatcggcg
     2881 aatgtactcc tgcatcattc ccgagctcga aacaccgggg cgtatgatac tgctggcagc
     2941 caccagggta atgtaatcct cgcaacgcag cttacgcagc aaacctttca tggcagggct
     3001 ctcaatgtaa aagcagcctg tggtatgcgc cgatttcagg agttgccgcg atgcctcgtc
     3061 ctgtttgaag cgtggaatgt cgtgaatatc cacttcgata cctttatttt tcaggatgat
     3121 ttcgcagctt tcgcggatgt gtccgatacc ccgctggctg agtatgtcga atttctcgaa
     3181 accgatatgc tcagcggtgt acatatccca ctgggtagtc tgataacctt tgggtggcat
     3241 atcgagagcc gcaaaagtgg tgatgggttc atcgctgata agcaccccac cggcatgaat
     3301 ggtacgcagg ttcgggaagc catccatttg ggcagccatt ttcacaatgc gtcggtgcac
     3361 gtcgtcgctg cgtccgtatt gcgcagggcg gtgactcagg ttttcgagtt catcgccggt
     3421 gaggccgtac acctttccca gttcgcgcag tgcagcacgg ctacggaaag tggacatggc
     3481 tcccagtaaa gcacaacgtt cttttccata cctatcgaaa agatagttat gcacctcgtc
     3541 gcggtcgctc cacgaataat cgatatcgaa gtcgggaggg ctgctgcgct tcgggttcag
     3601 aaagcgttcg aaatagagat cgagttctat ggggtccaca tcggtgatgc gcaggcaata
     3661 ggcagcaatg ctgttggcgc cactcccccg ccccacatga taaaagccgc ggctcatggt
     3721 gtagcgcacc aaatcccatg caatgaggaa atacgaggca aagcccatat tttcgatgat
     3781 ccccagctcg tgctccagac ggcgcatggc ttcggcattg tcggtaccat aacgcaatac
     3841 caatcctgcg taagcctcgc gggcaagcag gttgcggtcg tcgcgggcat tgccggtgaa
     3901 catcttgagg tttttcaccg aactgtcaaa gtgcagcgag caattgtgga gcaagttcag
     3961 ggtattgctt accacttcgg gataggcagc aaaagcagta cgtatggttg aaggactgac
     4021 aaagcggtcg ccccactggg ctaaatcggc aggcaaaagc ttcgagagca atgtattttt
     4081 atcgatggca cgcaggtgac gatgcaactc atacgattca tcgctgccga cactcagact
     4141 gtggagtacc aacattttcg taatgcggta ctttgcaacc agcaagggca gcaggtgggt
     4201 ctgccacagg gctacgccta catattcgtt atctttcaac ggtctgtcgg ggacacatcc
     4261 ggcagggaaa atcacgtatg cattgtcgaa atcaggaata gcatcgtgtt tacggccgtt
     4321 gatggcaatg tcgcttaaaa aactgttgat ttcacgcaaa cccgggttgt tgcgggcaat
     4381 acacacagcg atacgccggt tacccttgcg gatgtcggca ccgccaacgg gtttcagccc
     4441 cttgctttgc gccagaaaca cgaactcggg taaggcggtg gtgttgttga tgtcggtaag
     4501 tgcaatcgta tccatgtcgt tgcggagtgc cccgtcaatc aggtcgctga ggctgagcgc
     4561 accatagcgc aagctgtagt atgaatggca gttaaggtac atctgctgtt aaaattcgtt
     4621 tttatcattc aattcccgag gtcatcgagc cggtcatcga gcggagccga gatgatccga
     4681 gacgagccga gaaagtacaa agaacatctc cataatcagc cttcgattac cactccccta
     4741 cgctccgtta ctcaggctga caaaagcccc cctgtcatgc tgagtagtgg agcgcagcga
     4801 aacgcttatc gaagcatgga caggggagtc aaagaagtac aaagaacgtc tccataatta
     4861 cgctgcgatg tttcggcgtc tcgactccgc tcgacgaccg aaaacccgac tccgttcgat
     4921 gaccgaaaac ccgactccgc ccgatgactg aaaattcgac ctcaccataa cccgctcgag
     4981 aaccttattc ttaattttta atcatatttt cgccaataat ttaatcattt agataattat
     5041 ttgcaaatgt tttttaaatt gaaaaaatag cacatagtac aagtaatcgc tttgttacag
     5101 caaaaacatg aaaaaaccaa tgctattttg aataataata acgaatttaa tctattgctt
     5161 tgcaatcact tcgacagcct caccgatttg tgttacgaca acgaaatcat ttaaaaaggt
     5221 tattgcagta cagcacatgt tcatgctgaa tacggtatgc aacatccaga ataaccacac
     5281 ttacaaatag atacttgtgc ttaaaattat cacaaatcac attttataat gttataataa
     5341 agctaaaaca agctaaaaaa aattattatc tttaggcttg taaacttaaa taatcacagt
     5401 atgaaaaaaa ttttactttt cacttctctt acaacaatat ccttgtttac attttctcaa
     5461 ttggtaacta tttcgggcgt taaccaaatt cctcaggttg gcgataccat tcactacacc
     5521 aatataaaca cttttggttt tgaactggca ggtaccggaa ctgtgatgga taaaacatgg
     5581 gatctttcgt ttatgaccga agacgataat ttgtatttct tttatattga ccctgccgaa
     5641 acgccagatg cagcaagttt cccaacatcg aatcttgctg aaggcaccga tcaggccgaa
     5701 gggtattttt actttaaaac aggcgattat tatatggcac gcaaaggtgt aacgggcgaa
     5761 atgtatatcg attatgccaa cgacagtgca acaactttca cattccctat tactgctgga
     5821 cacgattacg attgtactta tgaagggatg ttgtatgcat caggtttgga aatgggaatt
     5881 gccaatggag atatccacat tgaagctgat gcacaaggaa cgcttatcac acccaatggt
     5941 tcaactttta caaacgtcct gcgtctccac attgttgaat cgttcgatgg gatgtatgat
     6001 gtaggaaccg gtacacccat ggttgtgctt tcggtggagg acgattatta ttattggttt
     6061 cacgaagatt atactagtcc aattttaatt tacggagtga ctacaaccga aagtttaggc
     6121 tcaaaccccg aggaaacaga ggttttacgc tatcaaccca ttaataatag ttctgttgtt
     6181 caaaacaact ctactaaaga aatgcatgta ttcccaaatc catcgtccgg acttgtattt
     6241 atcactaatt cgagccatta caccactttt aacatattcg atttaaacgg aaaactttta
     6301 aaaaccatca atgcttcatt aacgattgat ttgagcgctt ataacaatgg tatttatttt
     6361 atcgaaatga ttggagcaac acgatgcgat tatcaacgaa ttgtaattac acgataaaaa
     6421 tataataatc atccggagag ggacaagtaa tacttatttt aattgggcaa aaaacaatgg
     6481 cctaataaaa tttgaacact tcaatacgag tgaaagtatt ttaattaaca aattttattg
     6541 aatacgaaat ccatgattaa ataactataa ataatttata aaaattccct ttatcattac
     6601 caacaaaccc atacaagcat gagcaagaaa atacccgaag cttttaaaga atacctgcac
     6661 accctgcagg caaaacctgg gaaaccggtt tcactgattg atgacttcaa aacctgttgc
     6721 cacgaaaaac cggtgcctga gtacgacatc aaagcgatgc ttaaaaaaag tatatcgcag
     6781 ctttcgacca tgcaggacaa actgtatgcc cacgataaat acggtgtttt gattgtgctg
     6841 caggctatgg atgcagccgg aaaagacgga gcaatcaaac acgtaatgaa aggcttaaac
     6901 cctcagggag taaaggtatg gagttttaaa tcgcccaaca gcatagaact ggaccacgac
     6961 tatttttgga ggcactacag cgcgctgcct gcccgtggcg aaatcggcat tttcaaccgt
     7021 tcgcactacg aaaacgtgct ggttacccgc gtacaccccg aatatgtgat gaacgaaaaa
     7081 ctacccggta tcgaaaaagt ggacgatatc acccctgaat tctggcaatc gcgttatgaa
     7141 caaattaacc gttttgagaa aaacctgcac gaaaacggaa ccatcgtact gaaattcttc
     7201 ctgcatatat cgaaggaaga gcaacgcaag cgcttcctgg aacgtatcga caacccatcg
     7261 aaaaactgga aactatcacc ggccgactcg aaagagagaa aattgtggga caactacatg
     7321 catgcttacg aagaaatgat ttcggcaacc acctcaggca ttgccccgtg gtatattatc
     7381 cctgccgatg acaaatggtt tgcccgtttc tgcatcgcat cggtgattgt ggctgcaaca
     7441 aaaaaactcg acctgaaata ccctgaagtg gatgataaaa aacgtgctga attgttgaaa
     7501 attaaggaag atttgttgaa tgagtaaaaa ccgatatgta aaggatggaa gtattcggtc
     7561 acctcgactc cgctcggcga ccgaattaag caaccggcag gaaacccagt catcgcgcag
     7621 agccgagatg cccgggttaa aatttgtgtt atgctgctag cttttatttc tgacattcac
     7681 gaagatgtga gaatgctcga aaaagccctt cgaaccgctc acaaactggg cgcagaaaga
     7741 atcttatgtc ttggtgatat tactggctac tctccaataa atcaaaactt tgcatcagag
     7801 aagaatgcag cagcctgtat cgatttactt agggaacatt gcgaaatcag cattgccgga
     7861 aaccacgaca tgttcactgc acggcgtatg tcggaatttg cggcaggaat acattatcct
     7921 aaaaactggt atgaccttcc atttaccgaa aaacaacgac ttaccggaaa taaagtctgg
     7981 ctctacgaac atgaaataga cccactgctt ggccctgacc atttagaatg gcttcgtacc
     8041 ctacctgagc ttgcctatat agatgcctgc gaattgaaaa tttgcctgac gcattacatc
     8101 tatcccgatg tttcgggttc gctgcaaatg ggtcacgagc caaacagcga tgcatacgtg
     8161 catcgcaaca ggttctcatg tgacctgtat ctgtcggggc acctgcatcc caacggctgt
     8221 atcgttcagg ggaaaaagac agcgcatcgg tgggtaaata ttgattttgg cgagaagtat
     8281 tctgttgaaa acggagatta cctgtttgcg cctgcctgtc taaggcatcc tgtgaattgt
     8341 ggaattttat tgcttgatgc tgcaactaag tcggtacaga cggttcagat taagagttaa
     8401 cttgtttcag gctgttttac aaaatcatat taggcaggct aataacttgt tctcatcggg
     8461 attttattat ttttacacac taaattttgg gtaaagggtc atgcaaaaat ttctcgaaaa
     8521 catagcacac gaatttcaga caccttttca aaattacgtt ctggtgtttg ccgtcatgct
     8581 gtttattatc ctgctgattc cactattttt acgtaaaatc cgcatcccgg gaatcatcgg
     8641 actgattatt tcggggattc tcattggccc tcatggcttt aatatcatcg aaaaaaactc
     8701 agctgttgat ttattttcca ccataggctt gttgtatatc atgtttattg tcggtctgga
     8761 gcttgatttg gcaaaattca tcaagaataa atacaaaagt tttgttttcg gctttttcac
     8821 ttttgcgata cccattgcca tagggtttcc ggtatgtttt tacttactcc actacccact
     8881 caacaccagc atactggtag ccaccatgtt tgccacgcac acgctgatag cctaccctat
     8941 tgtcagcaag ctgggtattg caaaaaacga agcagtagcc atcaccgtgg gcggaacaat
     9001 tttcaccgat acagcggttt tggttgcgct tgccattatt accggttccg acacgggttt
     9061 tgccaacacc gaattcctga tcaggctggg aattgcattt gtggtttata tattatttgt
     9121 gatttttgtt gtgtaccggg tttcgcgatg gttttttaaa caccttgaag aagagcgcaa
     9181 ttcccagttc atatttgtcc tttccgttgt ctttctgtgt gcttttctgg ccgaaatagc
     9241 gggattagaa cccatcatcg gggcttttac tgcgggtttc acactgaatc gtctcatacc
     9301 gcaaacttcc agtttgatga accggctgga gttcgtaggc aactcgttgt ttatcccgtt
     9361 ctttttgatt ggtgtcggga tgattgtcga tccgggcgta ttcctcagtg gacccgaaac
     9421 cctgattgtt gccggaatac tcaccgtggt tgccctgatt gggaaatggc tggcagcttt
     9481 acttacgcaa tggacgttac ggtacaactc gctgcagcgc aagctgatat ttggtctcag
     9541 cagttcgcat gccgcagcaa ttctggctgt taccatggta gggcaccgca tgggaataat
     9601 cgacgacacc attttgaacg gaaccataat actgattctg ataacatgca tcgtagcctc
     9661 gatggttacc gaaaatgtgg gaaaaaagat tgtagttacc accgcatctg aaaccgatcc
     9721 tgaaactccg gtagccaccg aaaaaatact ggtacctgtg tcgaatcccg aaactatgcc
     9781 acggctgatc gatctggcac tgatgctgaa aaccacaaag aagcaggtgc cggtttacgg
     9841 ccttacggta gttgaagatg atgagactgc acgcgaaaaa ctgcggcaat cgcgcaaggt
     9901 acttaccgag ggagtaagct atgcagcagc ttcggaacag catttcgaag ttattgccac
     9961 cattgaccaa aatgttgttt cgggtattaa gcgggttgcc cgcgaaattt cggcaaccga
    10021 cattgtactc ggaagttcag ggaaaaccca catcagcgag ttatttttcg ggaaaacagt
    10081 aacccaaatt gtgagcagca ccgaccaggc aatatacgtg tataatgcag ccatgccact
    10141 gaatgtacac aaacatatcc acctggcttg cccgcgctat tccgaaaaag aacccggatt
    10201 tttcgattgg ctcgaaaaag tcgtgcgcat tgccaccctg ctgaatctga aaaccgatgt
    10261 gtattccgac aacgacacgt gcaaggccat tgagaaattt atcaaataca aaaaaatggc
    10321 cgccaacttt aaataccgcg atttcgacga ttgggaagac tttttggtgc tttcgcgcta
    10381 tttcgggcag gaagatttat tgattgtggt aacaccgcgt aagcccggag tttcgcatag
    10441 caatgtgctc gacggaatcc ctgcaaaatt ggcacgccat tttgcagaat acagttttct
    10501 ggtggtgtat cctgcagtga acaacaacga ggagttccgg aaatacagcg aaaactacga
    10561 cgcttcgctg atagaaaaag gaatcagatc ggtgaagaaa agcgccgaag aactgggaaa
    10621 attgtttaaa acgtgataat cttttaactt cgtaaactaa aaacataaag tatatgacta
    10681 gaaaaatcta tattctcgca ataattatcc ttacatcatt atgcattaaa gcccaaaaca
    10741 atatgacaaa tacattcgaa aaaaccacct gggaatcgct caacgacaac gccattcgta
    10801 tgataggcaa agactggatg ctaattggtg ccggcaccaa agaacaaggt ttcaacatga
    10861 tgactgccag ttggggtggc atgggatggc tttgggaaaa gcccgtgtcg tttatctttg
    10921 tcagaccaca gcgctacacc tatcagttta ccgaacgcga aaactactat acagtgtgtt
    10981 tctttagcga agaacaccgc gaagcattaa aaatctgcgg ttcgaaatcg ggccgcgatt
    11041 gtgataaggt gaaagaatca ggcctcaccc ccatctttac cccatcaggc tgtgtggcat
    11101 tcgaagaagc agtaataata ttggaatgta aaaagctcta tgccggtaaa atcgaagaat
    11161 caaactttgt tgatgccgaa gtagttaagt cgaaatatcc caataaggat tttcacaaaa
    11221 tgtatgttgg cgaaattgta aatgtgtgga agaaaaaaca ataatcaaat atttatccat
    11281 gaaaaaatta ctttttttat cgcttttagg tttagctttc cttagcctgc aagcacagaa
    11341 tttcgattac aagcccgttg ctgttgatgt atatcaactc gacaatggat taactgtgat
    11401 tttaagcgaa gatcatacgc aacctcaggt atttggtggg gttattgtgc gggcgggtgg
    11461 taaagatgat ccgcccgatg ctacagggat ggctcactat caggagcata tgctatttaa
    11521 aggcacacag gaattgggaa ccacaaattg ggaagcagaa aaaatacata tcgacagcat
    11581 ttttatgttg tacgatgagc ttggtaaaac caccgatgaa gtaacccgtg ctaatattca
    11641 gaaaaaaatt aacgagcaat cgctgaaagc taatgaatat gtgataccca acgaactgag
    11701 taacctgata aaagtgatgg gtgggaccga tttaaatgca ggcacagggc aggatatcac
    11761 aatttactat aatgcattcc ctccttctga gataaacaag tggctcgatt tgtacagcca
    11821 tcgtttcgaa aatcctgttt ttcgttcatt tcaggctgag ctcgaagtag tttatgaaga
    11881 gaaaaatatg tattccgaca tcttcatatt cccattgctg gaaagtttca ataagcaatt
    11941 ttttaaaaac catccctatg gccaacaaac cctgattggt tcggttgaag acctgaaaaa
    12001 cccatcttta tcgaaaatgt ttgaattttt caaaacctgg tatgtcccga acaacatggc
    12061 attgataata gtgggagact tcgacaaaga ggccattaaa ccgatgataa acgaaaaatt
    12121 tggcaaatgg gtgaaaagag agcttccgat acgccccgtt tatgaagaaa aagcatttaa
    12181 cgggagagag tttgcagagg ttaagctcag tccgataaaa ctcgcattgt taggcttcag
    12241 aactgtgccg gccggtcatt ccgatgaaat cgtattagaa gtgtgcaatg ccattctgtc
    12301 aaatcaaaat caaacaggat tgcttgataa actggtaaat aacaatgaat taatggcggc
    12361 tcaggcactg aatatgcctt acaatgacca tggagcttgt ttattcctta ttgttcccaa
    12421 aattctcgga caaaagcttt ctgatgccga acagctggtt atgaacgaaa tagacaagct
    12481 gaaatcaggt gatttcgacg attacatgat tgaaaatatt aaactggaat tgtacagaaa
    12541 ctaccttctc agcatggaat cgaacgatta taaagcaaac atgctggccg cagcgtttgg
    12601 cagatatcaa tcggtggaag accttctttc atacccgcaa aaactcatgg caatcactaa
    12661 agatgatgtc attaaaattg cccagaaata ttatggcgac aattacttag cattctattc
    12721 caatatggga ttcccgaaaa aagaaaaaat tgaaaaaccc ggttttaaac ccattgtttc
    12781 gaatacagaa tcagaatcgc agtatgccag gcattttaat cagattgctc cagttgaaaa
    12841 ggaaattacg tacatcaatt tcgaaaacga cattacccac gtagaaatta gtagtaacat
    12901 cgatatttac ggagttaaaa accccgctaa tgatattttt agcctgaaaa tcaagattaa
    12961 agcgggatat gaaaaaatcc ccatgctcaa atatgcaacc gatttgatga attatgccca
    13021 tcctgaaggc gttttatttg atgattttaa ggaaaaagta gccagactgg gatgtacata
    13081 ttctttttca ggtaacgaaa gttatactga gattgaaatt attggggttg aaagtaatat
    13141 ttctgatgca attcaattgg ttggtgaatt attgaacaaa ccggtaattg atcaggacaa
    13201 aataaatatt ttgtatgaag gtgagaaagc aaaccgcaaa atggagcgca gtgaaccaga
    13261 tgcagttgcg gatgcattat ttgaattcgt gaaatacgga aaggcttccg atttcatcga
    13321 cagactaagc ttaaaagaga taaaggcttt acaagctgat tcgcttgcca tagctttccg
    13381 tgatgcttgt aactacgggg ttgaatttca ttatgtaggg caaaacgata ttcaaactat
    13441 tgctgaccta tgcaaaacga ctttccgttt cgaagcatca aaattcactg agactcctgt
    13501 tgtaaagcca attgcaaagt tcagcgaaaa caccatttat tttgttcata agaagaaggc
    13561 cacccaaagt aaggtgtatt atctggctaa tgggtttgaa tatactcctg atattcaggc
    13621 aagcatggac gcattcaacc tgtattttgg tggggacttc tcgggtctgg tgcttcagga
    13681 aatacgcgaa taccgctcca tggcatattc tgccggtgca aaatacgttg ccgcgatacg
    13741 taaaggtgag caaacctact tcatggggta tgttggtact caggcagata aaaccgttga
    13801 agccatggtc gtatttgaca gtctcatccg tacgatgccg gagaaacctg aacgactaaa
    13861 aatgatacaa caatatctgc tgttatcttc atccagcgat cgcccccatt tcaggagcct
    13921 gagtgagcaa atacttgcat ggaagattca aggatttgaa agcgatcctg ctaaaatgaa
    13981 gaaagaatgc tacaccaatt tacgctttac agatatcagc ggtttttaca aagaacatct
    14041 ggccggcaaa ccacttgcga tatgcattgt tggtgataaa agcaagattg atatggcaga
    14101 attaagcaaa tacggcaagg tggttttcat taaagaaagc agtctgttca atgaataagc
    14161 aacaatctat ttcgcaggaa aatcgttgac aagtttcatg gctaaccatg ccatcatagc
    14221 cggggcatct ttcagtgcag cgggggaatg gtcgaaatcg ggattgtgaa gatttgtaac
    14281 ccctttccca tcagctgcaa tgcccatacg gtaaaaaaca gcaggaatct cgtgactgaa
    14341 atagccaaaa tcctcggcag taagtctggg ctccaatcta accacgcgat ctgctccgct
    14401 gaaatcggca gcaatttgca tacaatagtc agtgaattca ggattattca acacaaaggg
    14461 gtacccgtcc acaattttca gatgagcctc acacttgtac cgcggtgcaa tctcatttgc
    14521 caatatgtgc agttttcgct taacttctgc cctgaacaat tcgtcgaaac atcggaatgt
    14581 tccctctgct ctggcttcat ttgggataat attggttgcg ccatcagcat ttaatcgacc
    14641 aaagcttaaa acaaacggtt ttgaagtttt cgctgatttt tgcttgattg tttgtgtgct
    14701 catcacaaat tctgcaagtg ccatcacagt gttgctggta atgtcggggg tagcggcatg
    14761 tccgcctctc cctgaaaaac gaatataaat ctcatctgtt gaggccatat attctccctt
    14821 acaaaaccca aaactaccga ctggcaaccc gggcaataca tgctgtccga gaattagcat
    14881 gggtgcaaaa tcgcgaagta atccattatc aatcatagtt tttgctcctc ccggtagttt
    14941 ttcctcagca ggttgaaaga taaacaccac ttttccagag aaattgttct ccagtttctt
    15001 aaggattaga gctgttccaa gcaaggatgc agtatgaata tcgtggccac aggcatgcat
    15061 tattccctct ttctccgatc gtggtgcatg attcaatgat tcggttatcg gcaaagcatc
    15121 catatcggca cggagtgcaa cacataccgg tttttttgca gaaggcaagc agccttcaac
    15181 aacagcaaca acagagtttg gggcataatt caggtatgtt gtgataccat ttctgttaag
    15241 aatggaattt acatacatcg ccgtattttc ctcacaaaac gacagctccg ggttgcagtg
    15301 tatgtactcc tggaacgata ttgtttcagc cagaattgaa gagcttaact gtttaattgt
    15361 atcaatcatc atttaccaag taacatttta attccaacca aaataattaa agcgccaaat
    15421 acctttttca aagtatcggt attcatttgc acagcccatt ttgagctaaa atagctgcca
    15481 accataaatg ctacggcaat tattatggcg tatttcacat tcacataacc tgctttataa
    15541 taattgatga aggcaaaaat acctaccggt gcaagcatga atgcaagact ggttccctgt
    15601 gcagtatgct gagacgttcc gagcacatac acaagaaccg gaataacaac aatgcctccg
    15661 ccaaccccca taaacccacc aagcaccccg gctacaaggc cggcaagaac taaaagtatc
    15721 actgtgttaa aatccatgta tcttaaaagt caagattaag tgaaagataa aatatcccag
    15781 gcaaacgacg gttattttcg attccccatg cccagtcggc gcgaataaaa tatccaaaaa
    15841 gtttacttcg cactcccaca ccatatccgg caacaatagg gtaattgttg ttatcgataa
    15901 ttacagtcac cgggtaattt tcgtacacat cattatcaaa agcattatcg ccgctaaagg
    15961 ggttcaaacc gctccaggcg catcccatat caaaaaatcc tatcaattgc aaattttgaa
    16021 gaaatgccga attcagcggt ctgttcagaa tgtatgaaac aagtggccag cggatctctg
    16081 catttagcac agcgaagttg gtaccattgc ggacattttg cgagaaacct ctcatattgg
    16141 ttgcaacggc ctgatagaca taatttttat ccgggtcaat cctgaccgta ttgtcaaaag
    16201 tcggggtttt agtagagaaa tttatccagt tatcgatacc tccaagataa tatataagtt
    16261 tgcttcgtcc tgcagaaccg ctagctgcta ctctggcagc aattatcagg ttgcggtgca
    16321 ggggcaaata gtaccgaaaa tcgcctccaa aaactatcag gtccgattga tttttgtcga
    16381 tttgtcggta atattctgca aaaaccttat agcggaaacc attatagata tttatctgtt
    16441 tttccaaggt gttatcgaaa atatattcaa gttttgtccc tgcccagtaa ttatattcag
    16501 gttctgataa caaactgaaa taatcaagcg acaaatcggt cattttatca tgccgcccac
    16561 tgagggtaag ctgaactgat gaaacctgat taaa
//
LOCUS       DAOOQP010000064        16520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4275_length_16520_cov_5.987610, whole genome shotgun sequence.
ACCESSION   DAOOQP010000064 DAOOQP010000000
VERSION     DAOOQP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_16520_cov_5.987610"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLK75_07800"
     CDS             <1..564
                     /locus_tag="PLK75_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOY39189.1"
                     /translation="TRLNFDVVTCVNPWNIKELESLYQFLIDRNVKAWRLFTIAPMGR
                     AASNQELFLNSDQFDVLMKFIATKRQLHQNDIKFSCEAYVGKYELKVRDVPFFCRAGI
                     NIGSVLIDGSISACPNIDRNFVQGNIYKDNFFDVWQHRFDVFRNRDWNKTGICATCNE
                     YKYCAGNGFHYRECNNPEVMVCRKTKE"
     gene            complement(586..1779)
                     /locus_tag="PLK75_07805"
     CDS             complement(586..1779)
                     /locus_tag="PLK75_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39190.1"
                     /translation="MKTPGISFYTVLVLLLLGIISCKSKVDTELTVEISKKEGLIYFA
                     DNPQDSMLISTLAIVDSITMSEKGDYFDVFVEVWHQKFGDEKMYNHFKKAWLLEHEMN
                     GYRSLEHSPEIQVLKGCFEVIQKEALDVISKRLSEAGAKKVTVAKTEKNDVFVITMTN
                     APAKEIIQKLATTHGVFGIYETYNLIDVFQCFDAANKKLIENLGQFNDSQLIVGPVFE
                     LLAPSVLEGSEQSWKIAPGPVAGQCLNTDTARLIGLLKDAETEFPKELLFMWSAGASN
                     ISNEMSDLFMIKTYRGNSSMDNSHISYACVGSALKNNSTELKLEMNEEGKSLLRKLTL
                     ENTGLFLAVVIDNKVYNASSVNERNESGKFIFPVKLNSDQATELSYILSAGPILPNVH
                     VVSIK"
     gene            1894..2331
                     /locus_tag="PLK75_07810"
     CDS             1894..2331
                     /locus_tag="PLK75_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39191.1"
                     /translation="MKAFVCIMALFIAIPAFSQEFTKKEIKKHCNGFAKAVTDGDMEL
                     CLLYFDKEYLEVQHNDFLQGNTEQFLEEFLAGYDMKAPEGEQIIVPVFSEINSCKLIK
                     LKATSSDSYMVIFEIKLVNGITYLIETQVVKSPSGIGLVGAMG"
     gene            complement(2510..3412)
                     /locus_tag="PLK75_07815"
     CDS             complement(2510..3412)
                     /locus_tag="PLK75_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39192.1"
                     /translation="MQLLRNSAKPKPYLLIRRTDKNIGLGSYILLNLGHIHYAVENNM
                     IPVVDMQSYPNTYLENHEVGKVNAWELYFRQPANIGVADVKLRDCRVVDTAPAFVPCD
                     NMDFLTNHKLVQYWQKFTRKYLVLTKEIQAHMDEVYLNITGGSEKRILGVLCRGTDYL
                     STKPYGHPVQPEVDDVMAKAEQVMKQYNCSYLYLATEDKGILDRFREKFGDRLLYNSA
                     MRYGDTQDKRLSEFRFNRDRDAYLKGLEYLESLVILSRCNCLIGGRTNGSLCALMMSE
                     GYEYQYFWNLGRYRLDDKYSILSP"
     gene            complement(3455..4627)
                     /locus_tag="PLK75_07820"
     CDS             complement(3455..4627)
                     /locus_tag="PLK75_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOY39193.1"
                     /translation="MNKRIFLIIAMLLAFQTSRVVAQVVFNNNKPDSADIVIGKRYVI
                     HSEVLGEDREIYIHVPSEEVPDYDTVHYPVIYVFDGSTQFVSVVDIMNRFSTSLGDEI
                     CPKMIVVGIQQNDRQKDLGLVHDETEILTTNSFTGDPFTKFIEKELQPYVEANFNTMP
                     YRVLVGYSLGGLKAMNIIAYNTALFNAYIVIDPSVGNYNNQWFTSSIAKIQEADLSGK
                     SVFLAMAQTMQMGMDTIDIKKDSTTDSRHMRDIMASAEAFRKNSHSFDFYWKYYPDEY
                     HGSVPFIAEYDAFYAIFDWYNLQKLRYLFKSDDSPQAVKEAISLHFEQASGKIGFRLN
                     PPEHYLTDMISYFYFTGKFDKALAVSELLIEYYPDSKNSIRIHQILLDEQKSGNKM"
     gene            complement(4671..5273)
                     /locus_tag="PLK75_07825"
     CDS             complement(4671..5273)
                     /locus_tag="PLK75_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39194.1"
                     /translation="MNRMLMLITICTLAVFSSCSGNNSPEKQKTDTVKLQLTLNNMLP
                     VGWGVIYKATVDKIVEGNADDFDDTIEFGIIASKEYEKVQTGNTYIIGFTNSGEKATT
                     SYLPAIYGTVSNKNDIWLITDIGYVTESGLRTFEGTAIMSDGRALFVWDFADSEAFYL
                     DGVSTWDPKYLNKKICVEGELIQFIDGKSVIKNWKIVACE"
     gene            complement(5356..5607)
                     /locus_tag="PLK75_07830"
     CDS             complement(5356..5607)
                     /locus_tag="PLK75_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39195.1"
                     /translation="MKKLILILTSLTGLLSAFFIWLYVQRASLDYNDEGRIFCAENGV
                     VYHEQAKEVYGILALLSIMVTGILVIVIIKRKLHKRKKN"
     gene            complement(5743..6642)
                     /locus_tag="PLK75_07835"
     CDS             complement(5743..6642)
                     /locus_tag="PLK75_07835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOY39196.1"
                     /translation="MIKTFYKSKDKNFSLLQGNCIELLNQFDFKFDMIFADPPYFLSN
                     NGISVQSGKMVSVNKGDWDKSQGFEKDNEFNRNWLKACKEHLTENGTIWISGTFHNIF
                     SVAQMLTELDYKILNCITWAKTNPPPNLSCRYFTHSTEFIIWARKSKKSSHFYNYELM
                     KTINGGTQMKDVWNLPAIAPWEKSCGKHPTQKPLALLTRIILASTEKNAWILDPFTGS
                     STTGIAANLANRKFLGIDKEERFLNISKNRKLEIENPQIFGSFRKKLGGFENKNQLEL
                     FLAKEPEIEFERSLNFDRRIVNP"
     gene            complement(6652..7428)
                     /locus_tag="PLK75_07840"
     CDS             complement(6652..7428)
                     /locus_tag="PLK75_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39197.1"
                     /translation="MANNESWNRIFADYNILEHNFDENPFELSASQIKRSCQGFEDVN
                     QKEVRVLCKQDSRSDRPQIFVDNNLFILPVRNGFYKIIKGEGYVDIPEITSDVITYNS
                     ILDFKLESSEVGNSEMQHLDFAYASSLIRTFMEDDSLVLTIRGRKYTPKFEFNVGIHL
                     INTRSVQTEVDAGYEGRNKIVLIEAKNSSTNNTIIRQLFYPYKQWKIQTTKNVFLLFF
                     EKRQDIFHIWQYGFNNENDYNSITLIKSAKYKIDTAIEGA"
     gene            complement(7440..8360)
                     /locus_tag="PLK75_07845"
     CDS             complement(7440..8360)
                     /locus_tag="PLK75_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOY39198.1"
                     /translation="MTLKAKPFIKWVGGKTQLIESIEKALPVNFAENKNLTYVEPFVG
                     GGAVLFWILQQFPNITKAVINDINPDLTTAYKTIKKSPYELVKYLQTIQDEYLSLNEE
                     KRKEYYLNSRERFNTKSLDAVENTALFIYLNRTCFNGLYRVNSKGLFNVPFGRYANPK
                     ICDKQTIFADSEILQKVEILTGDFEQTLKYATNNCFFYFDPPYKPLSETSSFNSYAKE
                     DFNDNEQIRLGNFCKRIHLLGHDFILSNSDVKGKNPTDDFFDDLYEQFSIKRVFATRM
                     VNANAEKRGKLTELLITNNTTADINLLVSV"
     gene            complement(8546..9088)
                     /locus_tag="PLK75_07850"
     CDS             complement(8546..9088)
                     /locus_tag="PLK75_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOY39199.1"
                     /translation="MTTYISLLRGINVNGKNRITMDALRKSYGNLGFGSVRTYVQSGN
                     VVFTANDAQAEELAQKITQQIKKDFGVDVPVIVLTIDQLQHIVDSNPFLEHGDTNENS
                     LYITFLSSYPHNIDIPTIEAKKLRNEEVFFAGNAVYLYCHDGYGNTKLTTGFFETKLN
                     LHATTRNWKTTNTLLQMALQ"
     gene            complement(9107..9637)
                     /locus_tag="PLK75_07855"
     CDS             complement(9107..9637)
                     /locus_tag="PLK75_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39200.1"
                     /translation="MKLLFYFVLICTSFAACFAQTSDSNAGCNKEATAYWRQLADSGR
                     YNEAIEVLLDSIQKDNHKADRRNYWHVGQLYAFNNEYAEAIEYMKKSTGFFDKIFDRE
                     WRLYYNGTIAFLSRNKHKLNDCNEKLWNRHTQYYYVNACRLKSLNENFDKPYKIAYEK
                     PCELNCCCRYHPLKNQ"
     gene            complement(9657..10397)
                     /gene="blaOXA"
                     /locus_tag="PLK75_07860"
     CDS             complement(9657..10397)
                     /gene="blaOXA"
                     /locus_tag="PLK75_07860"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HOY39201.1"
                     /translation="MTEIRDDFKKYYDRFHVEGSFVLYDPQKDMYVFYNQDQCNQRFS
                     PASTFKICNSLIGLETGVITDENFVIPWDSVIRQNTCWNTDQNFKTAFKNSTVWYYQE
                     LARRVGGKQMKYWLDKASYGNADTSGGIDKFWLTGGLRISPQQQIDFLQRLHNNQLPF
                     SKRSMDIIKNVMIAKDTLGYVVRAKTGWGSDDNKDIGWYVGYIETKDNVYYFANCIQS
                     ADENNKDFANAPTDIVYLILCDLKLLSQ"
     gene            10729..14724
                     /locus_tag="PLK75_07865"
     CDS             10729..14724
                     /locus_tag="PLK75_07865"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOY39202.1"
                     /translation="MPGLKVLSFCVCLIMLVLTQVESSFCQIVINEGSNRNYSTISDE
                     DNEYPDWIEIFNSGDEEVSLNDWALSDNPANPHKWVFPNISLEPGAFKVIFCSSKNRR
                     PVTAFVNVAYIQDFAATASQWNTHQLTEAFYWDGVSNLLINICSYRSTGYTTNSVFNQ
                     TATDYYSTLYNFQDDSDDICYTKYGTRVKQRPNIQLNGVTIGNGTITNSPYDYPAPYG
                     NWYWSSRHQILITADDLIAAGLTEGMITSIAFDVVSTDPNTVYDYLDFSFKLITESEL
                     TSEFSPVDLHLTLHTNFSVSGEGETVTLYNPEQLEVSSLNVHCTGIDHSTGSFPDASG
                     NNHIFATPTPGESNNSSVYFDSYLLPPQFSVASGLFDLQVVVSITNPNPQESVIRYTT
                     NGDNPGPESPIYEGTPVVFYFSGVLKACAFSDDMLPSTSTTASYLIGINHTTPVISVV
                     TQNSNLYGDNGIFDNWWTDWEKSAYVEYFDTANNLVFSQPSAIQIDGGWGGSRSYPQH
                     SFRVELDHSVLGQAPVEYKLIPDRPQRIKYSKFYLRNGSNQFLTLPYKDAFQVTSLGE
                     GTLNHYSAMEPVSVYINGQYFGLYELREKIDEEYFEVYDDADEDSLDILTLSAWYSGV
                     LRATAGSVENFWDSYEAFLTLDPAQENFVESADHYFDMNSYADYIIAEAYTQNTDWPQ
                     NNIKMYRSEKTDYRFRFCLIDLELSMQPSGWSSTSDNPIDFLFSRDPNIPYINIWLRS
                     IQNEQFKNYFINRFADVMNTTYLTDKVLEKEQEMFSKMAAEMPKEFARWGDPGNVAGQ
                     MATFVGNHNILRSEYQSRTMFVREYITTGFDLIKQVDVGLDVIPENAGHIKISTIIPD
                     SLPWSGVYFDGNPVRLIAIPENGYEFLYWQPNDVLTEQNPEDSITLNISQSTEFIAVF
                     AQTETQGEITISEINYHSDSTRDAGDWVELLNFGDGQIDISNWKFTDSTLFHEFVFPE
                     GTVLQPGERLVLSSDPELFIEQHGSIDNFYEIEFGFSNSGEPLTLIDDNGFTYLSVHY
                     TDSLPWQEAADGHGRTLELRNDTLNPAIAANWFAGCMGGSPGQAYVACTEDIIFNEIN
                     YNSDGDADAGDWVELHNTGDEDIDISGWIFRDSDDAHSFEIPEATVINAGSYLVIFSD
                     PEKFSDRFPDVSNKAGPFDFGLSGSGEVIRLYGSTGNIYLSVVYSNLPSWPQGAAGNG
                     YTLEILDESGIFCDGDNWINGCLEGSPGVGFVPPCGNYIQENTSLKTISVLPNPTNGK
                     FRLNTSNLKPGNYMFTCANATGRIIFSRTETITDGTAFPEFTLEGLPEGIYFILVISG
                     NEKYSVKIVKQ"
     gene            15126..>16520
                     /locus_tag="PLK75_07870"
     CDS             15126..>16520
                     /locus_tag="PLK75_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOY39203.1"
                     /translation="MNSQLEKIIKKYNSDKNRLMDILLDTQAEFGYISKEATQSVAKI
                     LNMAVVDVEQTLSFYHFFTQKSRGTNTIYLNNSAVACMMGSEEVKKAFEEAAGCKFGE
                     NSADGKIGLYNTACIGLNDQEPAAIINGKMFTNLTPAVAKQIVTGLKEGKKVTDLYHK
                     NYGDGKNASAEILSMVNSNIKRRGQVILSDYTAGAALKTKLSSLQPKDVIDEVKKSNI
                     RGRGGAGFPTGLKWDFCSKSTGPKRYVLCNADEGEPGTFKDRVLLTERPEMLIEGMAI
                     AAYAIGSDEGIIYLRWEYKYLETFLEKVLADARKDNRLGQNVGGIKGFNFDIRIQFGS
                     GAYICGEETALIESMEGKRGEPRDKPPFPVDKGYMCLPTIINNVETFCSVVRIIEKGS
                     SWYKNIGTAESSGTKLLSISGDCAQPGVYEIEWGMTIAEMLEMVGAKDVQAVQVSGPS
                     GAVIGPKDFGRKICY"
BASE COUNT         4898 a         3242 c         3147 g         5233 t
ORIGIN      
        1 actcgcttga atttcgatgt ggttacctgc gtaaacccgt ggaatatcaa agagctcgaa
       61 agcctgtatc agtttttgat tgaccggaat gttaaagcat ggcgtctttt tactattgct
      121 cctatggggc gtgctgcttc aaatcaggag cttttcctca acagcgatca gtttgacgta
      181 ctgatgaagt ttattgctac aaaacgacag ttgcatcaaa acgatattaa attcagctgt
      241 gaagcctatg tcgggaaata tgagcttaag gtacgcgatg tgccattttt ctgccgtgcc
      301 ggtatcaaca ttggttctgt gctcatcgac ggctcaatat cggcatgccc taatattgac
      361 cgcaattttg tgcagggaaa catatataag gataattttt tcgacgtatg gcagcacaga
      421 tttgatgttt tcagaaaccg cgactggaac aaaaccggca tctgtgccac ctgcaatgag
      481 tataaatact gtgcaggtaa cggatttcac tatcgggaat gcaataatcc ggaagtaatg
      541 gtatgtcgca aaacaaaaga atagtttttc ggaagaagta ttaatttatt tgattgatac
      601 tacatgaaca ttggggagta taggtcctgc agacagtatg taacttaatt cggtagcctg
      661 atcagaatta agctttacag gaaaaataaa tttaccactt tcatttcttt catttacact
      721 tgaagcatta tacactttat tgtcgataac aactgcaaga aataagccgg tattctccaa
      781 tgtcaacttg cgtaaaagac ttttaccttc ttcattcatt tcaagtttca actcggtgct
      841 gttgtttttc agtgcagagc ccacacatgc atatgaaata tgagaattat ccatagaaga
      901 gtttcctcta taggttttta tcatgaaaag gtccgacatt tcgttgctga tatttgacgc
      961 acctgccgac cacataaaaa gaagttcttt cggaaactca gtttcggcat ctttaagtaa
     1021 gccgataagc cttgctgtat cagtgtttaa acattgccct gctaccgggc cgggagctat
     1081 tttccacgat tgttcgctcc cctcgagaac cgacggagcc agcaactcga atacaggccc
     1141 aacaattaat tgagaatcat taaattgacc taaattctca ataagctttt tattagcagc
     1201 atcaaagcac tgaaaaacat caattaaatt atacgtttcg taaatcccga aaactccatg
     1261 ggttgttgca agtttttgaa ttatctcttt tgcgggagca ttggtcatgg tgattacaaa
     1321 cacatcattt ttttctgttt ttgcgactgt aactttcttt gctccggctt cggatagccg
     1381 tttcgatatc acatcgaggg cttctttctg aatcacttcg aagcaacctt tgagaacttg
     1441 aatttccgga ctatgctcta gggacctata cccattcatc tcatgttcca gaagccaggc
     1501 tttcttaaaa tgattataca ttttttcatc accaaatttc tgatgccaca cttcgacaaa
     1561 aacatcaaaa taatcgcctt tttcagacat agtaatcgag tcgacaattg ctaacgtact
     1621 gattaacatg ctgtcctgag gattatccgc aaaataaatc aaaccttctt tcttgctaat
     1681 ttctacagta agttctgtgt caactttgct tttacaggaa attattccta acaatagcaa
     1741 aaccagtaca gtgtaaaatg agatgccggg tgttttcatg gatgttgtat ttgtctggta
     1801 aaattaagca ataattatat attatccaat taaacctaat gtaagaattg catttaatta
     1861 tctattttcg tccaattaaa aattaaagtt cctatgaaag catttgtttg tattatggct
     1921 ttatttatcg caatacctgc cttttcgcag gaattcacaa aaaaagaaat taaaaagcat
     1981 tgcaatggtt ttgcaaaagc ggttaccgat ggcgacatgg aattatgcct gctgtacttc
     2041 gacaaggaat atctcgaagt gcagcacaac gactttttgc aaggcaatac tgagcaattt
     2101 ctcgaagagt ttcttgccgg ctacgatatg aaagctcctg aaggggagca aataattgtt
     2161 ccggtatttt ccgaaatcaa ttcgtgtaaa ttaataaaac tcaaagcaac ctcctccgat
     2221 tcctatatgg taatttttga aattaaactt gtaaatggta ttacatacct catcgaaact
     2281 caggtagtaa agagcccatc aggaataggt ttagtaggtg caatgggata aagagttcat
     2341 ttctgcttca attactctgt cacgcctttc atcgagccgt tcatagagcg gagccgagat
     2401 gtcggcgaga tagtcatgac gctgaaacat agccaggggg ttaaaacgaa taattactta
     2461 acacatcttc gtagtcaccc ttcgattacc actccgcttt gccccgttac tagggtgaca
     2521 gaatgctgta cttatcatcg agccggtatc tgccaaggtt ccagaagtac tgatattcgt
     2581 aaccttccga catcatcagg gcacagaggc ttccattggt tcttccccct atcaggcaat
     2641 tgcagcgtga aagtatgacc aacgattcga ggtactccag ccctttcagg taggcatccc
     2701 tgtcgcggtt gaacctgaac tccgataagc gtttatcctg tgtgtcgcca tagcgcattg
     2761 cagaattata aagcaacctg tcaccgaatt tttcgcgaaa acggtcaaga atacccttgt
     2821 cctcggttgc cagatacagg tagctgcaat tatattgctt cattacttgc tctgcttttg
     2881 ccattacatc gtccacttca ggctgcaccg gatgcccata gggtttggtg ctcaaataat
     2941 cggtgcccct gcaaagcaca ccaagtattc ttttttcgct tccaccggtg atgttaaggt
     3001 acacttcgtc catgtgagcc tggatttctt tggtaagaac aaggtatttc cgggtgaact
     3061 tctgccagta ttgcaccaat ttatggttgg taagaaaatc catgttatcg cagggaacaa
     3121 atgcaggagc cgtatccacc accctgcagt cgcgtagttt tacatccgca acacctatgt
     3181 tagcgggttg cctgaagtac aattcccagg cattcacctt ccctacttcg tgattttcga
     3241 gataggtgtt tgggtagctt tgcatatcaa caaccggaat catgttgttc tcaacggcat
     3301 aatgaatatg gcccagattc agcagtatat acgaccccag tccgatgttt ttatcggttc
     3361 tccgtatcag cagatacggc ttgggttttg cggaatttct cagtaattgc atgtcagttt
     3421 acaagcttaa tagtattgtt tttcacagcc aaagctacat tttatttcct gacttttgtt
     3481 catcaagcaa tatttggtgt attcttatcg aatttttact gtcggggtaa tattcgatca
     3541 ataattctga aacagccagg gctttgtcaa attttccggt aaaatagaag tacgatatca
     3601 tatcggtaag gtaatgctcg ggagggttaa gccgaaatcc gatttttcca gaggcttgtt
     3661 caaaatgtaa ggaaatggct tctttcaccg cttgagggct atcgtccgat ttgaagagat
     3721 accgtagttt ttgcaaattg taccaatcga aaattgcata aaaagcatcg tattcggcaa
     3781 taaagggaac gctcccatgg tattcgtcgg gataatactt ccaataaaaa tcgaacgaat
     3841 ggctattttt tcgaaaagcc tcggcggatg ccataatatc gcgcatatgc cgtgaatcgg
     3901 ttgtgctgtc ttttttaata tctatggtat ccattcccat ttgcattgtc tgcgccatag
     3961 ccagaaaaac tgatttaccc gacaggtctg cctcctgaat tttggcaata gaactggtaa
     4021 accattggtt gttgtaattc cctacactgg gatcgatcac tatgtaagca ttaaataagg
     4081 ctgtattgta agcaataata ttcattgctt ttaatccgcc gagtgaatac cctaccagaa
     4141 cccggtacgg cattgtgttg aaatttgctt cgacataggg ctgcaactcc ttttcgatga
     4201 atttcgtaaa tgggtcaccg gtaaaactgt tcgttgtgag aatttcagtc tcgtcgtgta
     4261 ccagccccag atctttttgc ctgtcgtttt gctgtattcc aactacaatc attttggggc
     4321 aaatttcatc acccagcgaa gtgctgaacc tgttcataat gtcaacaacc gaaacgaact
     4381 gggtggagcc atcgaacacg taaatcaccg gataatgcac cgtatcgtag tcgggcacct
     4441 cttccgaagg aacatgaatg tatatttccc tgtcttcgcc aagcacttca gaatgaatta
     4501 catatctctt cccgataaca atatcagccg agtcgggctt gttattatta aaaactacct
     4561 gagccactac cctgcttgtc tggaaggcca acaacattgc gattatcaga aatattcgtt
     4621 tattcatggc attaatgtta gacatatctt gcacccctag ttttagacga ctattcacac
     4681 gctacaatct tccagttttt aatcaccgat ttcccatcga taaattgaat cagttcacct
     4741 tcgacgcaga ttttcttgtt caggtacttt gggtcccagg tgctaacccc atcgagataa
     4801 aaggcttcgg agtcggcgaa atcccatacg aacaatgccc tgccatcgct cattatggcg
     4861 gtaccttcga aggttctcaa tcccgattca gtgacgtaac caatatcggt gatgagccaa
     4921 atatcgtttt tgttgctcac ggttccgtaa attgccggaa gatagcttgt tgtggctttt
     4981 tctccggagt ttgtaaaacc gattatgtat gtgttgccgg tttgtacttt ctcgtattct
     5041 ttcgaagcaa ttattccgaa ttcgatagta tcgtcgaaat cgtctgcatt gccttccaca
     5101 attttatcga cagttgcttt gtagataacg ccccagccta ccggcagcat gttgttgagg
     5161 gtaagctgca acttcactgt atctgttttt tgtttttcag ggctgttatt tccgctgcac
     5221 gacgaaaaca cagcgagggt acaaatcgtt ataagcatca gcatccggtt catagtgcgt
     5281 ggtttaccat tgttaaaact caattcccaa ttttcagata aagataacac tatgaataac
     5341 gcaaattatt actatttagt tctttttgcg tttatgcaat tttcgtttta taataacaat
     5401 aaccaatata ccggttacca ttatactcag gagggcaaga ataccataaa cttcttttgc
     5461 ttgctcatga tacaccaccc cattctcagc acagaaaatc ctgccttcat cgttgtaatc
     5521 cagacttgcc cgttgcacat acaaccaaat aaagaaagcc gagagcaatc ctgtcaaaga
     5581 cgttagtatg agtattagtt ttttcatgat tctcaaggga gtaaagtaag cggtataatt
     5641 agttttcgat ttcgaggcac tgtcctataa agttatgcga aagtttatta gatgcagaaa
     5701 ctctgaaatg gccacttatt tgacaataaa acataccacg tgttatgggt ttacaattct
     5761 tctatcaaaa ttcaaacttc tttcaaattc aatttcaggc tctttcgcta aaaataattc
     5821 caattggttt ttattttcaa atcctccgag tttttttcta aaagaaccaa atatttgagg
     5881 gttctcgatt tcgagcttcc tattttttga aatatttaga aatctttctt ctttatcaat
     5941 accaagaaat tttctatttg caagatttgc agcaattcct gttgttgaac tacctgtaaa
     6001 tgggtctaaa atccaagcgt ttttttcagt agatgctaat atgattctgg tcaataatgc
     6061 caaaggcttt tgagttggat gttttccgca agatttttcc catggggcta ttgcaggcaa
     6121 attccaaaca tctttcattt gagtaccacc attaatggtt ttcattaact cataattata
     6181 gaaatgagag gatttcttgc tttttcgagc ccaaatgatg aactctgtag aatgggtaaa
     6241 atagcgacaa gaaagattgg gaggaggatt tgtctttgcc caggtaatgc aattcagaat
     6301 tttgtaatcc aattccgtaa gcatttgggc aacggaaaaa atattatgaa aagtgccgct
     6361 tatccagata gtaccatttt cggttaaatg ctctttacat gcttttaacc aattgcggtt
     6421 aaactcattg tccttttcaa acccttgcga cttgtcccaa tcgcctttgt tgacacttac
     6481 cattttacca ctttggacag aaattccgtt atttgataaa aaataaggag ggtctgcaaa
     6541 aatcatatcg aacttaaaat caaactgatt taaaagctca atacaattgc cttgtaaaag
     6601 actaaaattt ttgtcttttg atttgtaaaa agttttaatc atatgtaact ctcaagctcc
     6661 ttctattgca gtatcaattt tgtattttgc agatttaatt agagttatac tattataatc
     6721 attttcgtta ttaaatccat attgccatat atggaaaata tcctgtcgtt tctcgaaaaa
     6781 caaaagaaat acattcttag tcgtttggat tttccattgt ttatatggat aaaaaagttg
     6841 tctaattatt gtattattcg ttgaagagtt tttagcctca attaaaacta ttttatttct
     6901 cccttcatat ccagcatcta cctctgtttg tacacttcgc gtgttaatta aatgtattcc
     6961 aacattaaat tcaaatttag gagtatattt tcgccctcta attgtaagta caagtgaatc
     7021 atcttccata aacgttctaa tcaaacttga ggcataagca aaatcaagat gttgcatttc
     7081 agaattacct acttcagaac tttctagctt gaaatctaat attgagttat atgtaataac
     7141 atcacttgtg atttctggga tatcaacata tccttcacct ttaattattt tataaaaccc
     7201 atttctaact ggtaagataa ataaattatt gtcaacaaaa atttgaggtc tatcagatct
     7261 tgaatcttgt ttgcataaaa cgcgtacttc cttttgattt acatcttcaa aaccttgaca
     7321 agatcttttg atttgagatg cagataattc aaatggattt tcatcgaagt tatgttctaa
     7381 aatattatag tctgcaaaaa ttctgttcca agattcatta tttgccattt ttttattgat
     7441 taaacactta ctaataagtt aatatcagca gttgtgttgt tagttattaa aagttcagtt
     7501 agttttcctc gtttctctgc atttgcattc accattcttg ttgcaaaaac tcttttgatg
     7561 ctaaattgct cgtataaatc atcaaaaaaa tcatcggtag gatttttacc ttttacatca
     7621 gaattactta aaataaaatc atgaccgagt agatgaattc ttttacaaaa atttcctaat
     7681 cgaatttgct cattgtcatt gaaatcttct tttgcataag aattaaaact tgaagtttca
     7741 cttaagggtt tatatggtgg atcaaaataa aagaaacaat tattagtagc atatttcaat
     7801 gtttgctcaa aatcgcctgt caatatttca accttctgaa gaatttcact atctgcaaaa
     7861 atggtttgtt tatcacaaat cttagggttg gcatatcggc caaaaggtac attaaaaagt
     7921 cctttactgt ttacacgata taagccatta aagcatgtac ggttcaaata aataaacaat
     7981 gccgtatttt cgactgcgtc aagtgatttt gtattgaatc tttctcttga gtttaaatag
     8041 tattcttttc gtttttcttc attcagagac aaatattcat cttgaatcgt ttgaagatat
     8101 tttactaatt catacggtga tttcttaatt gttttatagg ccgttgttaa atcaggattt
     8161 atgtcattaa tcacagcttt tgtaatattc gggaattgtt gtaaaatcca aaacaaaaca
     8221 gcgcctccac caacaaacgg ctctacataa gtaagatttt tattttcagc aaaattgaca
     8281 ggcaaagcct tttcgatgct ttcaatcagc tgggtcttgc ctcctaccca ttttatgaat
     8341 ggttttgctt tcaaggtcat atttattttc ttcgaaaata gtggtttttc aagtatcctc
     8401 aaacaaagtt ttgatttttt ataaaaattt tgcacctaat gttgttggga ttaaggttgt
     8461 tatttcaccg ttctgtcttt cgcacaacaa ccccgttttg cagcgatatt accgccatgg
     8521 ttaaaacgca tcaacgcact tattcttatt gcaaagccat ttgtaaaaga gtattcgttg
     8581 ttttccaatt tcttgtggtt gcatgcaggt tcagttttgt ttcgaaaaaa ccggtggtca
     8641 attttgtatt gccatatccg tcatggcagt acaggtacac agcattgccg gcaaaaaaca
     8701 cctcttcgtt gcgcagtttc tttgcttcaa ttgttgggat atcaatgttg tgcgggtaag
     8761 acgacagaaa agtaatgtac aaggaatttt cgttcgtatc gccatgttct aaaaacgggt
     8821 tgctgtcgac gatatgttgt aattgatcaa tggtaagaac gattacaggt acatcaacgc
     8881 caaagtcctt tttaatttgc tgtgttattt tctgtgcaag ttcctcagcc tgtgcatcat
     8941 tcgcggtaaa aacaacattt ccgctttgca cataggttct cacactacca aagcccaaat
     9001 tcccgtacga cttccgtaac gcgtccatcg tgatacggtt tttcccgttt acattaattc
     9061 ctctgagtaa tgaaatgtat gtcgtcattc gcattctggt tgattgctat tgatttttaa
     9121 gtggatggta ccggcaacag cagtttaatt cacatggctt ttcatacgct attttatatg
     9181 gtttatcaaa attttcgtta agcgatttta gtctgcacgc attcacataa taatactgag
     9241 tatgcctatt ccacaatttt tcgttgcaat cattcagctt gtgtttgttt ctgctaagaa
     9301 aagcaatcgt tccattatag tataatcgcc actcccgatc gaagatttta tcaaagaaac
     9361 ctgtcgattt cttcatgtat tcaatcgcct cagcgtattc attattgaaa gcatacagtt
     9421 gccctacatg ccaataattt ctacggtcgg ctttatggtt gtctttttga atactgtcca
     9481 acaaaacttc aatggcttca ttatatcttc cgctgtcagc caattgcctc caataggctg
     9541 tggcttcttt gttgcatccg gcgttactat cgcttgtttg agcgaaacag gcagcgaaag
     9601 aagtgcaaat taatacaaag taaaaaagca gtttcatatt gcatcagatt tcattctcat
     9661 tggcttaata gtttcagatc gcaaaggatt aaatatacaa tatcggttgg tgcatttgca
     9721 aagtccttgt tgttttcgtc agccgattga atacagttag cgaaatagta cacgttgtct
     9781 tttgtttcga tataaccgac ataccaacca atatctttat tgtcatcact cccccagcct
     9841 gtttttgccc ttaccacata acccagggtg tcttttgcaa tcattacatt ttttattata
     9901 tccattgagc gcttagaaaa agggagttgg ttgttgtgaa gtcgttgtaa aaaatcaatt
     9961 tgctgttgtg gtgaaattct aagcccgcca gtaagccaaa acttgtcgat gccacccgaa
    10021 gtatcagcat taccatagct tgctttgtcg agccaatatt tcatttgctt gccgccaacc
    10081 cgccttgcaa gttcctgata ataccaaact gtcgagtttt tgaaagcggt tttaaaattc
    10141 tggtcggtgt tccagcaagt gttttgtctg attacgctgt cccagggaat tacaaaattc
    10201 tcatctgtaa taacacctgt ttcaagcccg atgagagaat tacaaatctt aaaagtcgag
    10261 gctggtgaaa atctttggtt gcattggtct tggttgtaga aaacatacat atctttttga
    10321 gggtcgtaaa gcacgaatga accctcaaca tgaaatctgt cgtaatactt tttgaagtca
    10381 tcacgaattt cagtcacatg tttttgttta caagccgata ctgcaacaac agaaaaaatc
    10441 aacaataaca gggaatgttt cacaatggta atattattac tgctaatgta aacagataga
    10501 ttcgaaattc aaattatttg agttttttta atctgtgact taaagttatt ctgatacatc
    10561 ggagaagtct ttgagggtat ctaatggaaa aaaccaactc attagagaat tttgtataaa
    10621 atttatttca cattcattca ttattatgga ttgcaaatta tttaattttt attctgacag
    10681 acaatttcta aattgcgaag tattacaata gcattttaac atttatttat gcccggatta
    10741 aaagtattga gtttttgtgt gtgccttatt atgttggttc ttacacaagt agaaagctct
    10801 ttttgccaga ttgtgataaa cgagggatcg aaccgcaact atagcacaat ttctgacgaa
    10861 gacaatgagt atcccgactg gattgaaatt tttaactcag gcgatgagga agtgagtctg
    10921 aacgattggg cactgagcga caatccggcg aatcctcata agtgggtttt ccctaatatt
    10981 tcacttgagc caggtgcatt taaggtaata ttttgtagta gtaaaaacag acgtccggta
    11041 acagcatttg taaatgttgc ctatattcaa gattttgcag caacagcctc gcaatggaat
    11101 acccatcaac ttaccgaagc cttttactgg gacggagtat caaatttgct gatcaatatt
    11161 tgctcatacc gttcaaccgg gtacactaca aactctgttt tcaaccagac tgcaaccgat
    11221 tattactcta ccctctataa ttttcaggac gatagcgatg atatatgcta tacaaaatat
    11281 ggaaccagag taaagcaacg cccgaatata caattgaacg gagttaccat tggtaatggc
    11341 acaataacca attctcctta cgactacccg gcgccttatg gtaactggta ctggtcgtcg
    11401 cgccatcaga tactgattac tgccgacgac cttattgccg ccggacttac tgaaggaatg
    11461 attaccagta ttgcgtttga tgttgtttcg accgatccca ataccgtgta cgactatctt
    11521 gatttttcgt ttaaacttat tacagagagc gaacttacct cagaattttc accagtcgat
    11581 ctacacctca ccttacatac gaatttttcg gtttcaggcg aaggtgaaac tgttacgctt
    11641 tacaaccctg aacaattaga agttagttcc ctgaatgtac attgcacagg aattgatcat
    11701 agcacaggtt cgtttcctga tgcttccgga aacaatcaca tctttgctac accaactccc
    11761 ggtgaatcaa ataatagcag tgtatacttc gattcctacc tcctgccacc acaattctcg
    11821 gtagcatcag gtttatttga cttacaagtg gtggtttcaa taaccaatcc aaatcctcag
    11881 gaatcagtta tccggtacac aaccaacggc gataatcccg gaccggaatc cccgatttac
    11941 gaaggaaccc ctgttgtttt ctatttttcg ggagttttaa aagcctgcgc attttccgat
    12001 gacatgctgc caagtacctc caccacagca tcctatttaa tcggtatcaa tcacactact
    12061 ccggtaatat ctgttgttac ccaaaactct aatttatatg gagataatgg gatttttgac
    12121 aattggtgga ccgattggga aaaatcggcc tatgtagaat atttcgatac tgctaacaat
    12181 ttggttttct cgcaaccatc agcgatacag atcgacgggg gttggggagg cagcaggtcg
    12241 tatccgcaac attcgttcag ggttgaactt gaccattcgg tgcttggtca ggcccctgtc
    12301 gaatacaaat taattccgga ccggccacaa cgcataaaat acagtaagtt ttatctgcgc
    12361 aacggaagca atcagtttct taccttgcca tacaaagatg cttttcaggt tacatcgctg
    12421 ggcgaaggaa cgctgaatca ttattcagca atggaacctg tatcggttta tatcaacgga
    12481 cagtactttg gcctctacga attaagagag aaaattgatg aagaatattt cgaggtatat
    12541 gatgatgccg acgaagattc tctcgacatt ctcacactga gtgcgtggta cagtggtgta
    12601 ctcagggcaa cagccggctc tgttgaaaac ttctgggata gctacgaagc atttctcact
    12661 ctcgatcctg cgcaggaaaa ttttgttgaa tcggccgacc actatttcga catgaattct
    12721 tatgcagact atatcattgc cgaagcctat actcaaaaca ccgactggcc acaaaacaat
    12781 attaagatgt acagatccga aaaaaccgat tatagatttc ggttctgcct tattgacctt
    12841 gaattatcga tgcaaccaag tggatggagc tcaaccagcg acaatcccat tgatttcctc
    12901 tttagtcgcg atccaaacat cccttacatt aatatttggc tgcgaagcat ccagaatgaa
    12961 cagtttaaaa actatttcat caatcgcttt gccgatgtaa tgaacacgac ctatcttact
    13021 gataaggtac ttgaaaaaga gcaggagatg ttttcgaaga tggccgccga aatgcctaaa
    13081 gaatttgcgc ggtggggaga tccgggtaat gttgcgggtc agatggcaac ttttgtcggg
    13141 aatcataata ttctcaggtc ggaatatcaa tcaagaacta tgtttgtaag agaatatatc
    13201 acaaccggtt ttgaccttat aaagcaggtt gatgtgggtt tggatgtaat tcccgaaaac
    13261 gctggccata taaaaatcag tacaataatt cccgactcac tgccatggtc aggggtatat
    13321 ttcgatggaa atccggtacg tctgattgcc attcccgaaa acggctatga gttcctctat
    13381 tggcagccga acgatgtgct taccgaacaa aacccggaag atagcattac cctgaatatt
    13441 tcgcaaagca cagaatttat tgctgtattt gcgcaaaccg aaactcaggg agaaataaca
    13501 atatccgaaa ttaactacca ttccgacagc acacgcgatg caggcgactg ggtggaacta
    13561 ctcaattttg gtgatgggca aattgacatt tctaactgga aatttaccga cagcacactg
    13621 tttcatgaat ttgttttccc tgaaggcacc gttcttcaac cgggcgaaag gttggtactt
    13681 tcttccgatc ccgaattgtt tattgaacag catggtagca tcgataattt ttatgaaatt
    13741 gaatttggct tcagtaattc cggcgaaccc ttgacattga ttgatgataa cggttttacg
    13801 tacctgtcgg tacattacac cgatagtttg ccctggcagg aggcagctga tggtcacgga
    13861 cgcacgctag aactgcgcaa cgacacgctt aatccggcta tcgccgcaaa ttggtttgca
    13921 ggttgcatgg gcggttcccc cgggcaagcc tatgttgcat gtaccgaaga tattattttc
    13981 aatgaaataa actataattc agatggcgat gccgatgcag gcgactgggt tgaattgcac
    14041 aataccggcg atgaggatat cgatatttcg ggatggattt ttcgcgacag cgacgatgcc
    14101 catagctttg aaatacctga agctactgta ataaatgcag gatcgtatct ggttattttt
    14161 tcagacccag aaaaattctc tgatagattt cctgatgtaa gcaataaagc gggtccgttt
    14221 gactttggtt taagtggttc gggcgaagta atcaggttat acggcagcac aggaaacatc
    14281 tatctttcgg ttgtttacag taatctacca tcatggccac agggagctgc gggaaatggc
    14341 tatacacttg agattctcga tgaaagcggc attttttgcg atggcgataa ttggattaat
    14401 ggttgtttag agggttcgcc cggggttggt ttcgttcctc catgtggaaa ttatattcag
    14461 gaaaatactt cattaaaaac catttctgtt ttgccaaacc cgacaaatgg taaattcagg
    14521 ctaaatacat caaatcttaa accgggtaat tacatgttta cttgcgctaa tgccacagga
    14581 agaattattt tcagcagaac agaaaccatt actgatggta cggcattccc ggaattcaca
    14641 ctcgaaggat taccggaggg aatttatttc atattggtaa tttcaggaaa cgaaaagtac
    14701 tcagtaaaga ttgtaaagca ataagcttgc ggctatatat tttaatgttc aaataagtaa
    14761 tctgagtttt acaaaaactt caattataat ctttgtttgt cagcattcat attatttgga
    14821 ataaaaaatc aggtaaaaaa caaaaaccta ttttgggtaa atggaagaac ttctccaata
    14881 acttttgcat aaaataatgc ttgttttaca attcatcaat aatgtgagca gcaaccttat
    14941 ttttacaaga taacaacagt cctcctgatg tttgtgcaag actagtgtat ttgaatgtct
    15001 aattttgaat atttccaaat tagaataatg tgttattaaa gcattgctca tttttagaat
    15061 aataatatat ttggcacaat ttaataatta gttaattttt taacaaataa taaagcttat
    15121 acagaatgaa tagtcaatta gaaaagatta ttaagaagta taactcagac aagaacaggc
    15181 ttatggatat tctattggat acccaagctg agtttggtta tatctcgaag gaagcgacac
    15241 aaagtgttgc taaaattctc aacatggcag ttgttgatgt tgaacagaca ttatcatttt
    15301 accatttttt tactcagaaa tcacgcggga ctaacactat ttatcttaat aatagtgctg
    15361 tagcctgtat gatgggtagc gaagaagtaa aaaaagcatt tgaggaagcc gccggatgca
    15421 aattcggaga aaacagtgct gacggaaaga ttggtttgta caatactgct tgtattggtt
    15481 tgaacgatca ggagcctgct gctataatca acggtaagat gtttaccaat ctcacacctg
    15541 ctgtagcaaa acaaattgtt acaggtttga aagaaggtaa aaaagttaca gatttgtatc
    15601 acaagaatta tggtgatggc aaaaatgcat ctgctgaaat tctgtcaatg gttaacagta
    15661 acattaaacg cagaggtcag gtaattttaa gtgactatac tgcaggtgct gctcttaaaa
    15721 ccaagttatc atcattacaa ccaaaagatg taattgatga agtaaaaaaa tcaaacatcc
    15781 gcggacgcgg tggagcaggt tttccaacag gtttgaaatg ggatttctgt tcaaaatcaa
    15841 caggtccgaa gcgttatgtt ttgtgtaatg cagatgaagg cgaaccgggt acatttaaag
    15901 atagagttct cttgacagaa cgtccggaaa tgttaatcga agggatggct attgcagcgt
    15961 atgctattgg ttcagacgaa ggtattatat atttgagatg ggagtacaaa tatctagaaa
    16021 catttctgga gaaagtctta gcagatgcaa ggaaagataa cagacttggt cagaatgtcg
    16081 gtggaatcaa aggattcaat ttcgacataa gaattcagtt tggttcaggt gcttatattt
    16141 gcggcgaaga aactgcactc atcgaatcaa tggaaggtaa gagaggcgaa ccaagggata
    16201 aacctccctt ccctgtagat aaaggttata tgtgtttacc aactatcata aacaacgtag
    16261 aaacattctg ctcagttgta agaataattg aaaaaggcag cagctggtac aaaaatattg
    16321 gtacagcaga atcatcaggc accaaattat taagtatttc tggtgattgc gcacaacctg
    16381 gcgtgtatga aattgaatgg ggaatgacta ttgctgaaat gctagaaatg gttggagcaa
    16441 aagatgttca ggctgttcag gtaagtggtc cttcaggtgc agttattgga cctaaagatt
    16501 ttggcagaaa aatatgttat
//
LOCUS       DAOOQP010000065        16501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4280_length_16501_cov_5.249970, whole genome shotgun sequence.
ACCESSION   DAOOQP010000065 DAOOQP010000000
VERSION     DAOOQP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4280_length_16501_cov_5.249970"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /gene="gpmI"
                     /locus_tag="PLK75_07875"
     CDS             <1..1373
                     /gene="gpmI"
                     /locus_tag="PLK75_07875"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOY39204.1"
                     /translation="SEVGHLNIGAGRVVYQDLVRINKEVKSNAISNNPILKQAFSYAK
                     AHNKAVHFVGLIGNGGVHSMSSHLFKLCDITRDYNLDKVFVHALTDGRDTDPMSGKGF
                     TEDLLQHIQGTKVKVASLAGRYYTMDRDKRWERIKQGYDALIYGIGEYSTDLIGSIQK
                     SYNNGITDEFIKPIIMVDENGKPVAVIQDNDVVICFNFRTDRLRQISIALTQKDMPEA
                     GMKTIPLQYYTMTRYDESFENVHVIYDKQNVQNTLGEVLSQHGLKQLRIAETEKYAHV
                     TFFFSGGREDAFDGETRIMIPSPKVATYDLQPEMSAYKVKDAVIEQLDKNENDFICLN
                     FANGDMVGHTGVYNSIFKAVQAVDTCVGEVVAAARKNNYEVVIIADHGNADYATNDDG
                     SPNTAHSLNPVPIIVISNRVKRVTSGILADVAPTVLHLMEIQAPIEMTGKVLIEKYSC
                     GCCGCS"
     gene            1482..2411
                     /locus_tag="PLK75_07880"
     CDS             1482..2411
                     /locus_tag="PLK75_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOY39205.1"
                     /translation="MRNLLFGIVYLIALPTVLFAQKTTAQYIEEYAAMAVREMERSGV
                     PASITLAQGILESGSGNSRLALEGNNHFGIKCHTWNGEKIYADDDAKNECFRKYKSAD
                     ESFRDHSDFLKTNSRYSFLFEIDKTNYKEWAKGLKKAGYATSPTYAEALINLIEKYEL
                     YRYDTQGSLIKPHNNRKKTQSKPAEEHFTLNPYGRDIYYINNVPFILAGDNESIEKIC
                     SEFDMMPWQVRKYNDIEKGDSITTGDRIYLKPKRNKVDKEHSTHTVSDGETMYSISQQ
                     YGIKLNQLYRKNKMEPGTEPKPNTVLSLRKSIR"
     gene            complement(2468..5404)
                     /locus_tag="PLK75_07885"
     CDS             complement(2468..5404)
                     /locus_tag="PLK75_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOY39206.1"
                     /translation="MNMVDIERFESLREKLTGMLYCDNVMRTLYATDASAYREKPIAV
                     IVAANKNDIKNAILFARENNLSIIPRTAGTSLAGQVVGNGIVVDISKHFNGVIELNVA
                     EKWVKVEPGVVLDELNKILAPHKLFFAPETSTSNRCMIAGMVGNNSCGAHSLIYGSTR
                     DHTLEIECLLSDGSEVVFKSLTRSEFFEKCELQNLEGNIYRNIRDILSIPENQKEIEN
                     QYPDKEIKRRNTGYAIDLLLDTEVFGESEEKFNFCKLLAGSEGTLAFTTAVKLNLVDL
                     PPKQKALVCIHTHTLREAFEANLIALKYQPGSVELMDKKVLDLTKANITQSRNRIFVE
                     GDPEAILIVEWAREDMGEIKNLAMAMETEMRNSGYGYAFPMITGSDIPKVWELRKAGL
                     GVLSNMPGDMKPVPVIEDTAVAPRLLPDYMREFEQILQRLKLSCVYYAHIGTGELHLR
                     PVLNLKDAAHVEMFRTIAFETALLVKKYKGSLSGEHGDGRLRGEFIPLVLGDKVYEML
                     KQVKNAWDPHLVFNPNKIVDTPAMNTQLRYSPGKATPEINTVFDFSSTGGILRTAEKC
                     NGSGDCRKSSLIGGTLCPSFQYTRDEKHSTRARANIIREVLTNSTQKNPFDSKDIYDV
                     LDLCLVCKACKSECPSGVDVAKLKMEFLQHYYDAHGIPLRTRAIAYLPVIHRLFSPFS
                     GVANFFMANAFFSGILKSIIRFAPKREFPLMHKPSLTHWTKKYSGTLTPSSPKKTVYL
                     FNDEFTNYLDANTGIAAIRLLTSLGYEVIIPKTVESARTWLSKGLVRTAKNRANKNIA
                     LLKDLISAETPLIGLEPSAILGFRDEYPDLAFPELKEAAKQLAKHSYLFEEFIVSEID
                     NGNISSAMFTDEERTIVFHGHCQQKALTTTAATRKALSLPCNYKVSEIPSGCCGMAGS
                     FGYEKEHYETSMGIGEMVLFPSIRNRPEGSIVVAAGTSCRCQIKDGTSVQALHPAEVL
                     WMALRR"
     gene            5575..6492
                     /locus_tag="PLK75_07890"
     CDS             5575..6492
                     /locus_tag="PLK75_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOY39207.1"
                     /translation="MKPKDAITLVLKGLAMGTANVIPGVSGGTIAFITGIFERLINAI
                     KSFNLTALKLLFKGDFRGLLKHIDFFFLLWLFLGIGIAIISVAKLFEYLFANYPVYLW
                     SYFFGLVLASVVFVSITIKKWSFSVITTYLIGAAIAVVISFMTPASENTNVFYLLLCG
                     AIAACSMILPGISGSFVLVLLGNYELVMIKAVTQLRFEILLPIIIGAAVGLLGFSYVL
                     SWVFRKFRNQTIAGISGFILGSLLIIWPWKDAVMKQYGSIEKVSGYIWKLPELNVEMF
                     VAIAIMIFGIACVWIMEYYASKKEKPDGE"
     gene            6482..7246
                     /gene="aroE"
                     /locus_tag="PLK75_07895"
     CDS             6482..7246
                     /gene="aroE"
                     /locus_tag="PLK75_07895"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOY39208.1"
                     /translation="MENKKEFGLIGYPLGHSYSKVYFTEKFRSLGIDNASYQTFPLSD
                     IGKMHNLVKSHKNLVGLNVTTPYKEIVIPYLNEVDASVLKIGTVNAISIFRNEESYHM
                     KGYNTDVDGLRKLFSGFNFSGIEKALILGSGGSGKTTAFVLKEMGIHCRFVSRHPSGN
                     ETVSYTSLNAKLMADNLLIVNASPVGMHPLSYNFPDIPYEYISEKHTCIDLVYNPEIT
                     EFIKKCREKGAQTANGTVMLYEQADKAWEVWQRDVF"
     gene            7303..7374
                     /locus_tag="PLK75_07900"
     tRNA            7303..7374
                     /locus_tag="PLK75_07900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7336..7338,aa:Arg,seq:cct)
     gene            7760..8533
                     /locus_tag="PLK75_07905"
     CDS             7760..8533
                     /locus_tag="PLK75_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOY39209.1"
                     /translation="MSNEESSIHDFDFNLICEYFSAIERQGPGSEDVTLKALSFIDNL
                     TDRSQIADLGCGTGGQTMVLAQHAPGKFTGIDLASVFIDLFNRNAQRMNLSSRVQGII
                     GSMDALPFGNEELDLIWSEGAIYNIGFERGLAEWHRFLKPGGFVAVTEACWFTGSRPA
                     EIENFWLDAYLGIDTIPNKLLQLQQSGYVPVACFILPENCWRENFYVPQAAAQHVFLL
                     KHAGNKTAEMLVANERRESQLYEKYSRYYGYAFFIGKKI"
     gene            9035..10171
                     /locus_tag="PLK75_07910"
     CDS             9035..10171
                     /locus_tag="PLK75_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOY39210.1"
                     /translation="METPFTFGKIAVKSNFTDREEEAVRLVTNFSSKINTILISPRRW
                     GKSSLVSKAAQLASKKDSRLRFVFIDLYNVRSEEEFYQLLAKNILLASSTKLEELRDK
                     AGKFLGKLIPKLSFSPDPNSEFNISFDWDEVKKDTDDILNLPENLTNEKNIRFVVCID
                     EFQNIASFGDSLAIQKKLRSNWQKHQNTSYCLYGSKRHMMIDVFTSASMPFYKFGNII
                     FLQKIEKNALQRFIVKRFKDTGKTIDKASAGLIADKAECHPYYVQQLAQLTWLRTGSV
                     CTPEIVEEAHQGLLMQLDFLFQSITDDLGNTQVNFLKALLEGVTQLSASETLQTYKLG
                     TSANVLRIKSSLENKEIIDLYGGKISFMDPMYKCWLKTVYFRIK"
     gene            complement(10702..12759)
                     /locus_tag="PLK75_07915"
     CDS             complement(10702..12759)
                     /locus_tag="PLK75_07915"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOY39211.1"
                     /translation="MKKLVLSLVSFLLFAGIIYSQHTAMNGTWKMIASESSFLDYYKE
                     LTLDITISNNSAEIITRMGPKLKFEEKLTFSTDGKVNKSEIKDGTFSTNIHMGLRLPV
                     GSKKEIKAIWENDNTITLTEKYDYYASQGKKHGESVYRYQLSEDGKRLKCTITRPTRP
                     NDSDLVFVFKRSNTQNAFIHRMADDWDIGKHLPEQAFWISLQGVVNQNEPLLYFTFAK
                     GYPFNYTTELAEYLETKRNFSFTSLSTVEQGIETFKSHINGYIIWDKNVRTSLIVAYT
                     LAGLENAVIITDDMIPLMQQHGFKQVADFRGQFNGMSDFEIYSWAKEQYWNRCSREVI
                     TWLGGQHGTVLMPACADYGMMKKTFFSDLSARPTDTLEYNLTRSLFAGMKPLSQVWGW
                     HSYKKDLEEQMTTLLSSYALTSDGLNTQPNTSFLIHIPLSEGFVFKNNHNIEAGKTYM
                     PEKKIYISFIQSDGIGIGAWFEPGRGSIPYAWEVTMKMLEMSPVLLEYYYAQATPNDY
                     FIAALSGSSYMYPKAFPPQWLPAELKRAHDLMKKLDLNVFEIMDYSEGGTETCDNNLP
                     KSVVDEYYRSMPDAIGFINGYRASNTFTVRDKKPFVSYDYYLAAEKPEAEVVADLEEL
                     AAINSERPYFLLVHVRESSDIARVKSICDKLSENIEVLPLDIFLKMAGENPTFKEYYY
                     QGK"
     gene            complement(12900..13367)
                     /locus_tag="PLK75_07920"
     CDS             complement(12900..13367)
                     /locus_tag="PLK75_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39212.1"
                     /translation="MSRRKLFLIGFICFLLCNVIILSCTDQHDIGRLPVEGLNNIYVK
                     LDQDNEFDHSTGIHYEIIFGNGQVIVEKTLLTGCSDYIEDLSDFTAGSCDSVIFVTFF
                     DSTHVFAVYDLKTGNGHPRSSKETDYKTSYFFGDSLIQELNSCKPELKADWKH"
     gene            complement(14196..15806)
                     /locus_tag="PLK75_07925"
     CDS             complement(14196..15806)
                     /locus_tag="PLK75_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOY39213.1"
                     /translation="MIQNKIFPLLKEIPEEHCIPIPFNQTEYLLNGELMHWEGPTEKV
                     FSPVYFYENNTLINLIGSYPLMDGKTAMMSLHAAIEAYNNGQGTWPKMTISQRIGCMR
                     KFILLMKEQRDIIVKLLMWEIGKNLTDSQKEFDRTVEYINDTIEALKDMDREASRLKI
                     TQGVIAQIKRSPLGVVLCMGPFNYPLNETFATLIPALIMGNTVIFKPPKLGVLLHAPL
                     LRAFQEAFPKGVVNTVYGEGREVIAPIMESGKVNVLAFIGSCKVADTLKKQHPHPHLL
                     KSVLGLEAKNPAIILKVANIENAVKECVTGALSFNGQRCTALKILFVHDSIYDDFINR
                     FISEVDSLKSGMPWEKDVNITPLPEDYKPKWLEDLIEDAVNNGAVICNKNGGIMEKSL
                     FFPVVLSGVSSKMQIYHLEQFGPVIPVVKFHDVEEPLEYVVTSNFGQQASIFGEDPVI
                     ISVLVDTLVNQLCRVNINSQCQRGPDVFPFTGRKDSAEGTLSVSDALRVFSIRSLVAL
                     KENDTNKKILTDILNSKNSNFLSTDYIL"
     gene            16045..16485
                     /locus_tag="PLK75_07930"
     CDS             16045..16485
                     /locus_tag="PLK75_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOY39214.1"
                     /translation="MAENNSLFGEFKPISYEEWHAQVIKDLKGADYGRKVVWNTLEGV
                     SLEPFYRFEDVKDLAYTDALPGEKPFVRGSKPSHSWRIRQDFRAADPAEANRKALKAL
                     QKGAQSLGFVTCTPLTYQQLETLLNGIDLTTVPVHIVMFRVVDK"
BASE COUNT         4872 a         3264 c         3502 g         4863 t
ORIGIN      
        1 atagtgaagt tgggcatctg aatattggcg cagggcgtgt agtgtatcag gacctggtaa
       61 gaataaacaa ggaggtgaaa agcaatgcta tcagcaataa tccgatacta aaacaagctt
      121 tttcgtatgc taaagcccat aataaagctg tgcactttgt cggactcatt ggtaatggtg
      181 gtgtgcattc tatgagctca catttgttta agctatgtga tattacccgg gattataacc
      241 ttgataaagt attcgttcat gcgctgacgg atggacgcga taccgaccct atgagcggca
      301 aaggatttac cgaggatttg cttcaacata ttcaaggcac caaagtgaaa gttgccagcc
      361 ttgcaggtag atattataca atggatcgtg ataaacgatg ggaaaggatt aaacaaggct
      421 acgacgcttt gatttacgga ataggagaat atagcacaga tttaatcgga agcattcaga
      481 aatcatataa taatggcatt acagatgaat ttattaaacc catcattatg gtggatgaaa
      541 acggaaaacc tgtcgctgta attcaggata acgatgtagt gatttgtttt aacttccgca
      601 ccgacagact taggcaaata tccattgccc ttacacaaaa ggacatgcct gaagcaggta
      661 tgaagacaat tccattgcag tactatacca tgacgcgtta tgatgagtcg tttgaaaatg
      721 tgcatgttat ctacgataaa caaaacgtac aaaacaccct tggagaagtt ttatcgcaac
      781 atggtttaaa gcagctcagg attgctgaaa cggaaaaata tgcccatgta acattttttt
      841 tcagcggggg cagggaagat gcattcgatg gagaaacccg catcatgata ccatctccta
      901 aagtggcaac ttatgatttg caacccgaaa tgagtgctta caaggtaaaa gatgctgtga
      961 tagaacaact tgataaaaat gaaaatgatt ttatctgcct gaattttgca aatggagata
     1021 tggttggcca tacaggcgtt tataattcaa tatttaaggc agtgcaagcg gttgatacat
     1081 gtgtcgggga agttgttgct gctgcaagaa aaaacaatta tgaggttgta ataatagctg
     1141 accatggtaa tgccgattat gctaccaatg atgatggtag cccaaatact gcccattcgt
     1201 tgaatccggt tccgataatt gtaataagca atcgggtgaa gcgggttact tcaggaatat
     1261 tggcagatgt tgccccaaca gttctacacc tgatggaaat acaagcacca atcgaaatga
     1321 ccggaaaagt actcattgag aagtatagtt gcggatgctg tggatgcagt taaatttgtt
     1381 tgagattcgc aatattaacg aacccgtgtt aaaaagtaac attaacatgg taataacagt
     1441 ttacatgttc ggtctatttt tgcacaatac aaagtcttct catgaggaat ttgctttttg
     1501 gaatagttta tttaattgct ctacccactg tgttgtttgc acagaaaacc acggcccagt
     1561 atattgagga gtatgctgcc atggctgttc gcgaaatgga gcgttcaggc gtacctgcaa
     1621 gcattacact ggctcaggga attttagagt cggggagtgg aaattcacgc cttgccctag
     1681 aagggaacaa ccattttggg attaaatgcc atacatggaa tggagaaaaa atatatgcgg
     1741 atgatgatgc taagaatgag tgctttagaa aatacaaaag tgcagatgaa tcgtttcgag
     1801 accatagcga ttttttaaaa acgaacagta gatacagttt tttattcgaa atagataaaa
     1861 ctaattataa agaatgggca aaaggcttaa agaaagcggg ttatgcaaca tcgcccacat
     1921 atgccgaagc attgattaat ctgattgaga aatatgagct atatcgttac gatactcagg
     1981 gaagcttgat taaacctcac aataaccgta aaaaaacaca atcaaaacct gcagaagaac
     2041 actttacgtt aaatccttat gggagagata tttactatat aaataatgtt ccatttattc
     2101 ttgcaggcga caatgaaagc atcgaaaaaa tatgtagtga atttgatatg atgccttggc
     2161 aggtacgtaa gtacaacgac atagagaaag gtgacagtat tacaaccggc gacagaattt
     2221 atctgaaacc aaaacgaaat aaagtcgaca aagaacattc gactcatacc gtttctgatg
     2281 gcgaaaccat gtactctatt tcgcaacaat atggcattaa actcaatcag ttatatcgca
     2341 agaataaaat ggaacctgga acagagccta agccaaatac ggtactgtca ctccgaaaat
     2401 ccattaggta aatttcaatc agttttgcgc agactgggaa tttaatcaac gagttaattc
     2461 ttcttattta tcttctaagg gccatccaca aaacttcagc cggatgcaat gcctgtacac
     2521 ttgttccatc cttaatctgg caacggcaac tggtacctgc agcaacaaca atagagcctt
     2581 caggccggtt tctaatactg ggaaaaagca ccatttcacc tatccccatt gaggtttcgt
     2641 aatgctcttt ttcatatcca aacgaccccg ccatgccaca gcaacctgag ggaatttcac
     2701 taactttata attacagggg aggcttaaag cttttcgtgt tgcagcagtt gttgtgagtg
     2761 ctttttgctg acagtggccg tgaaaaacaa ttgttctttc ctcgtcggta aacatagcag
     2821 atgatatatt cccattatca atttcagaaa caataaattc ttcgaacaaa tacgaatgtt
     2881 tggccagctg ctttgccgct tctttcaact ccggaaaggc aagatcagga tactcatctc
     2941 tgaaacccag tatagcagaa ggctcaagac cgatgagtgg tgtttcagca gaaattaaat
     3001 cctttaatag ggcaatattt ttgtttgcac gatttttagc agttcttacc agtcctttcg
     3061 aaagccaggt acgtgcactt tcgactgttt tcggtataat aacttcatac ccaagcgaag
     3121 taagtagcct tatagcggca atacctgtgt tggcatccag gtaatttgta aattcatcat
     3181 tgaaaagata tactgttttt ttgggcgatg aaggcgttag tgtgccggag tattttttag
     3241 tccagtgtgt taaggatggt ttatgcatta acgggaattc acgttttggg gcaaagcgga
     3301 tgatagattt cagtattcct gaaaaaaaag catttgccat gaaaaagttt gcaacaccgc
     3361 tgaatggact gaacaacctg tgaattaccg gaaggtatgc gatagctctg gtccgtaatg
     3421 gaatcccatg agcatcgtaa taatgctgta aaaactccat ttttagtttt gctacatcaa
     3481 caccggaagg acattccgat ttacaggctt tgcatacaag gcagaggtca agaacgtcgt
     3541 aaatatcttt cgaatcgaac gggttctttt gggtggagtt ggtaagtacc tctctgataa
     3601 tattggcacg cgctctggtt gaatgttttt catcacgagt atattggaaa cttgggcaca
     3661 atgtaccgcc aatcagcgat gatttgcggc agtcgccaga gccgttgcat ttttcagcgg
     3721 tgcgcaaaat gccaccggtt gaggagaagt cgaaaactgt gttaatctcc ggagtagcct
     3781 ttcctggaga atatctcagt tgagtgttca tggcaggagt gtccacaatt ttgttagggt
     3841 taaacaccag gtgcggatcc catgcgtttt ttacctgctt aagcatttca taaaccttgt
     3901 cgccaagaac cagaggtata aattcacctc tgagcctgcc atcgccatgt tctccactca
     3961 gcgacccttt gtattttttt acaagcaaag cagtctcgaa ggcaattgta cgaaacatct
     4021 caacatgagc agcgtctttc aggtttaata caggtcttaa gtgcaactct ccggtaccta
     4081 tgtgagcata atatacacaa ctcagtttca atctctgaag aatttgttca aactcacgca
     4141 tgtagtcggg gaggagtctg ggcgccactg cagtgtcttc aattacaggg acaggcttca
     4201 tatctccggg catgtttgaa agcaccccca ggccggcttt tctcaattcc catacttttg
     4261 ggatatcgct accggttatc atcgggaaag catagccgta acctgaattg cgcatttcag
     4321 tttccatggc cattgccagg tttttgattt ctcccatatc ttcacgtgcc cattccacaa
     4381 tgagaattgc ttcaggatct ccctcaacaa aaatgcggtt tctgctttgg gtaatattgg
     4441 cttttgtaag atccagcacc tttttgtcca tcaattctac tgaaccaggt tgatatttca
     4501 atgctatcag gttggcttca aatgcctctc tgagggtgtg ggtatgtatg catacaagcg
     4561 ctttctgttt tggcggcagg tcaacgagat ttagttttac agcggtagta aaagccaaag
     4621 tcccttcaga ccctgcgaga agcttgcaga aattaaattt ttcttctgac tctccaaaaa
     4681 cttctgtatc aagcaacagg tcaatggcat aacctgtatt acggcgttta atctccttat
     4741 cggggtattg attttcaatt tctttctgat tttcgggaat ggaaaggata tcacgaatgt
     4801 ttctgtaaat attgccttca aggttctgca attcacactt ctcgaaaaac tctgaacgtg
     4861 tcaaactctt aaaaacaact tcgctgccat cacttaacaa acactctatt tcgagggtgt
     4921 ggtcgcgggt acttccataa ataagagaat gtgctccaca actgttgttt ccaaccatac
     4981 cagctatcat gcagcgattt gaagtggagg tttcgggagc aaaaaacagc ttatggggag
     5041 caagtatttt attcaattcg tcaagaacca caccaggctc aacttttacc catttctcag
     5101 cgacattcag ctcaatcacc ccattaaaat gcttcgaaat gtcaacgaca atcccattgc
     5161 caactacctg acccgctagc gatgtgcccg ctgtgcgggg tatgattgaa agattgtttt
     5221 cacgagcaaa tagaattgcg ttttttatat cgtttttatt agcagcaaca ataactgcaa
     5281 tgggtttttc gcgatatgcc gaagcatcgg tggcgtacag agtgcgcatc acattgtcgc
     5341 aatacaacat tcctgtaagc ttttccctca gagactcgaa tctttcaata tcaaccatat
     5401 tcataaatag ggattaaaat aaagctcaaa attataaaac ttagtggcaa cgcataaaga
     5461 taattgtaac aaaaaaataa ctgttgataa acacacagca ttcattaata agttttagaa
     5521 tgcagataag caataatgct aaaatttact attttcgcgg tcaatttcga aactatgaaa
     5581 ccaaaagatg caattacact tgttctaaaa ggacttgcca tgggtacggc aaatgtgata
     5641 ccgggtgttt cgggtggcac tattgctttt ataacaggta tttttgagcg cctaatcaat
     5701 gctataaaat catttaatct cactgcactg aaattgctat ttaagggaga tttccgtggg
     5761 ctcctgaaac atatcgattt cttttttctg ctgtggttgt ttttggggat tggaattgca
     5821 ataatttctg ttgccaagtt attcgagtat ctttttgcca actacccggt ttatttatgg
     5881 tcatatttct tcggcttggt attggcttcg gtagtttttg tcagtattac cattaaaaaa
     5941 tggtcattct cagttattac aacatattta attggagctg caattgctgt tgtaatatcg
     6001 tttatgacgc cggccagcga aaataccaat gttttttacc tgttgctttg tggggcaatt
     6061 gcggcttgta gtatgattct gccaggtatt tcagggtcgt ttgtactggt attacttgga
     6121 aattatgagc tggttatgat taaggcagtt actcagcttc ggtttgagat acttcttcca
     6181 atcataatcg gagctgcagt aggtttgcta ggcttttcat atgtgttatc gtgggtgttt
     6241 cgtaaattca gaaatcagac catcgcagga atatcagggt ttatcttagg gtcgctttta
     6301 attatttggc catggaaaga cgctgtaatg aaacagtatg gcagcattga aaaagtttca
     6361 ggctacatat ggaaacttcc tgaattgaac gttgaaatgt ttgttgccat tgcaataatg
     6421 atttttggta ttgcctgtgt ctggattatg gaatattatg cttcaaaaaa ggagaaacct
     6481 gatggagaat aaaaaagaat ttggtttgat cggttacccg ttggggcatt cctattcaaa
     6541 agtttatttc acagaaaagt tcagaagttt gggtattgat aatgcaagtt atcagacttt
     6601 tccgctatcc gatattggaa aaatgcataa tcttgtaaaa agtcataaaa accttgtggg
     6661 tttgaatgtg acgaccccat ataaagaaat tgtaattccc tatcttaacg aagtggatgc
     6721 ttcggttttg aaaataggaa cagtaaatgc catcagcata ttccgaaacg aagagagtta
     6781 tcatatgaaa ggttacaata cagatgttga tggtttacga aagctcttct caggcttcaa
     6841 cttctccgga attgaaaaag cgctgatact tggctctggt ggttcaggca aaacaacagc
     6901 atttgtactg aaagaaatgg gaattcattg ccgttttgtt tcacgtcacc catcaggtaa
     6961 cgaaaccgtt tcgtatactt cgctgaatgc aaagcttatg gctgacaacc ttctcattgt
     7021 gaatgcaagt ccggtcggta tgcacccttt gtcgtacaat tttcctgata ttccctatga
     7081 atatatctcc gaaaaacaca cctgcatcga tctggtgtat aatcctgaaa ttactgagtt
     7141 tattaagaaa tgtagagaga aaggtgcaca aactgctaat ggcacagtga tgttgtatga
     7201 acaggccgat aaggcatggg aggtttggca gcgcgatgtg ttttaattac ggtttctctt
     7261 tgtagcattt gataaattgt tttatttttg tgtcgtagaa aaggccctat agttcaatgg
     7321 atagaacgga agtttcctaa actttaaatt caggttcgat tcctggtggg gctacagagt
     7381 gaacacacca aagccacgag agtggctttt tttcgttaga aaaagaatcg aagtttaccc
     7441 tgagttacga agggcttcct ggtggggcta cagagtagac acaccaaagc cacgagagtg
     7501 gcttttttca ttgggaaacg aagtgaagtt cgaaattgta aagttttaaa atttctgatt
     7561 atctttgtca taataaaact tttggatgtt taataattgt acggataggg tgatgtaaat
     7621 aggccggctg atgtcgtgct tatccctttt gtttattgca gtggctgcta gccgttgtgt
     7681 tatcatttta aatgtataat ccggatgatg tgattcttta attattccgt atatcgcaca
     7741 tccacaacaa agtaatatca tgagtaacga agaaagttca attcacgatt ttgatttcaa
     7801 tttaatttgc gaatattttt ctgctatcga aaggcagggg ccgggaagtg aagatgtaac
     7861 actgaaagcc ttaagtttta ttgataacct gacagaccgg tcacaaattg ccgatttggg
     7921 ctgtggcacc ggtggacaga ccatggtact ggcgcagcac gcaccgggaa aattcacagg
     7981 tatcgatttg gcgtcggttt ttatcgattt attcaaccga aatgcgcaaa ggatgaatct
     8041 tagcagcagg gttcagggca ttattggttc gatggatgca ttgcctttcg gtaacgaaga
     8101 actcgacctg atttggtcgg aaggggctat ttataacatc ggcttcgaac gtggactggc
     8161 agaatggcac cggtttctga aaccaggagg ctttgtggca gtgactgagg cttgctggtt
     8221 taccggcagc agacctgccg aaatcgaaaa cttctggctg gatgcatatc tgggtatcga
     8281 cactatacct aataaactgt tgcagttgca gcagtcgggt tatgtgcctg tggcgtgttt
     8341 tattctgccc gaaaactgct ggagagagaa tttttatgtt ccacaggcag cagcgcaaca
     8401 cgtttttctg ttgaaacatg ccggtaataa aacagccgaa atgctcgtcg ccaacgaacg
     8461 acgcgaatca caactgtacg aaaagtacag tcggtactat ggatatgctt tctttatcgg
     8521 aaagaaaatt tagtttttta gtcaggcgtt gaatttgttt gacgcctgtt ttttactctt
     8581 ttatcttgac atcgtatgca acgattaccc tgttatgctg aggactaatt attggggcaa
     8641 taaaagcaat atgaaatgac aaggtatatg gaatcagaat acagaataca gaattcagaa
     8701 gtgggcaact ggcaattatc aagcattctt ctctatctgg atttttagaa ccttctcggg
     8761 gtagcagatt tctttagcac gacgaaaagc cccttctgta ttctgtctcc tgtattccca
     8821 aatttaacaa tacgtcattg gtagtagtgc aacggcaatt tatacttcgg caggccatta
     8881 gtataaattg cccttgcgct acacagactg acaaattcat aagaactgca tggaattgct
     8941 ttgatttact taatggtaag ttacaaaata gtaagttaca aatttgtaag tttgtaaaat
     9001 attaattata tttgtgaaat aaaaacatca gaaaatggaa acgccattta catttggtaa
     9061 aattgcggta aagtcgaatt ttactgacag ggaggaagaa gcagtccgac tggtgacaaa
     9121 cttctcatct aaaataaaca cgatactaat ttcaccgagg cgctggggta agtcgtcgct
     9181 ggtgagtaag gcggctcagt tggcatcgaa aaaggacagc cgtctcaggt ttgtctttat
     9241 cgatttatat aatgtgagaa gcgaggagga gttttaccag ttactggcaa aaaacatcct
     9301 gcttgcctca agtacaaaat tggaagaact tagggacaaa gccggtaagt ttttgggaaa
     9361 actgattccg aagctttcgt ttagtcccga cccaaacagt gagttcaata tcagtttcga
     9421 ttgggatgaa gttaaaaaag acacagatga tattttaaat ctgcctgaga atctaaccaa
     9481 cgaaaaaaac atacggtttg ttgtttgtat agacgaattc cagaatattg catcatttgg
     9541 cgactcactg gccattcaga aaaaactccg gtcgaactgg cagaagcatc agaatacttc
     9601 gtattgcctg tatggcagca aacgacacat gatgattgat gttttcacgt cggcatcaat
     9661 gccattctat aaatttggta atattatttt ccttcagaaa attgaaaaaa atgccttgca
     9721 aaggttcatt gtcaagcgtt ttaaagacac cggcaaaaca atagacaaag ccagtgccgg
     9781 actgattgct gacaaggctg aatgtcaccc gtactatgtg cagcaactgg cacagcttac
     9841 ctggctacgc accggcagtg tatgtacgcc cgaaatagtt gaagaagcgc atcagggtct
     9901 gcttatgcag ctcgactttc tgtttcaatc aattacagac gatttgggca acacccaggt
     9961 gaactttctt aaagcactac tcgaaggagt tacacaactc agtgcaagcg aaacactcca
    10021 aacttataag ctgggaactt cggccaatgt acttcgtatt aaatcgagtc tcgaaaacaa
    10081 ggaaatcata gatctatacg gtggcaaaat cagttttatg gatcctatgt acaagtgctg
    10141 gctcaaaacc gtgtatttca gaattaaata atttcccctt caaagggggc ataattctca
    10201 cagcaacagt cacatttgct gccattgcgg tacacgtcat cgcgagcaaa gccacacgtc
    10261 atcgcgagca aagcgaagcg atctccttac ccgtagacgt tgctgtagcc gcctgtctta
    10321 atgccgccag cttcgttacc ctgccgcgga cactgaggtt ctcgaagtgc ccgttgaaat
    10381 gcagattgct ttgtcctcgt tcctcgtccg cgcaatgacg ggcttgaaaa cgtcatcgaa
    10441 gccgagatgg agccgagacg cagaagtccc tttggtgagc ccgtcgagta cagcggctcc
    10501 gagtcccgtt ccggaaactt ggagcgggag ggaaaagaca gaacgtgttc agaattaaat
    10561 aactccccct tcgaaggggg gtcaggggga tgtgtaatat acgctgcccg cagcacgtac
    10621 tgtacacttc gagacgattc tcgttcctcg aatccctcag tatccagtaa cgtaccgcat
    10681 ttacacaaag cattttcatt actacttccc ctgataataa tactccttaa atgtcgggtt
    10741 ttcgccggcc atttttagga aaatgtcgag gggcagcact tcgatgtttt cgctcagctt
    10801 gtcgcagatt gactttacac gggcaatgtc gctcgattca cgcacgtgta caagcagaaa
    10861 atatggtctt tcggaattaa tggccgccag ttcctcaagg tcggccacca cttcagcttc
    10921 aggtttttcg gcagccagat agtagtcgta cgaaacaaag ggctttttat cgcgcacggt
    10981 gaatgtgttc gacgcccggt atccattgat aaatccgatg gcatcgggca ttgagcggta
    11041 atattcgtca accacgcttt tgggcaggtt gttgtcgcag gtttcggtac caccttcgga
    11101 gtaatccatt atctcgaaaa cattcaggtc gagttttttc atcaggtcgt gggcacgttt
    11161 cagctcggca ggcagccatt ggggaggaaa ggccttcggg tacatatacg acgaacccga
    11221 taatgcagca atgaaatagt cgttgggcgt ggcctgtgca tagtagtact cgagcaatac
    11281 cggcgacatt tcgagcattt tcatagtaac ttcccatgca tagggtatgc tgccccttcc
    11341 gggttcaaac catgcaccaa tgccgatgcc gtcggactgg atgaaactga tgtagatttt
    11401 cttttcaggc atgtaagttt tgcccgcctc tatgttgtga ttgttcttaa aaacaaaacc
    11461 ttccgacaag ggaatatgaa tcagaaaact ggtgttgggc tgggtgttta acccatcgga
    11521 agtgagcgcg tacgatgata ggagtgtggt catttgttct tcgaggtctt tcttgtagga
    11581 atgccacccc caaacctgcg acaagggttt catgcctgca aacagcgaac gggtgagatt
    11641 atactcgagt gtgtcagtag ggcgggctga caaatcagag aaaaacgtct ttttcatcat
    11701 tccataatcg gcacaggcag gcataagcac cgtgccatgc tgaccaccca gccaggtaat
    11761 cacttcgcgc gagcagcggt tccagtattg ttcttttgcc caggaatata tttcaaagtc
    11821 cgacattcca ttgaactgac ccctgaaatc ggcaacctgt ttgaatccat gctgttgcat
    11881 taatggaatc atgtcgtcgg ttataattac tgcattttca agaccggcca gtgtgtaggc
    11941 tactatgagc gaagttctta catttttgtc ccagataatg tagccattga tgtgcgattt
    12001 aaaagtttca attccctgtt caactgtgct cagcgatgta aatgaaaagt tcctctttgt
    12061 ttcgagatat tcggccagtt ctgtcgtgta gttgaatggg tatcccttgg caaaggtaaa
    12121 atacaataag ggttcgttct gatttaccac accctgaagc gaaatccaga aagcctgttc
    12181 cggcaggtgc ttgccaatat cccagtcgtc ggccatgcga tgtataaacg cattttgggt
    12241 attgctgcgc ttaaacacaa aaacaaggtc ggaatcgttg gggcgtgtag ggcgcgttat
    12301 tgtgcatttc aaccgttttc cgtcttccga caactgataa cgatacactg actctccatg
    12361 ctttttcccc tgtgaagcat agtaatcgta tttttcggta agcgttattg tgttgtcgtt
    12421 ttcccagatg gctttgattt cttttttcga tcccacaggt agcctgagac ccatgtgtat
    12481 gttggtggaa aaggtgccgt ctttaatttc acttttattg actttgccat cggtgctgaa
    12541 agtcagtttt tcctcgaatt tgagttttgg ccccatacgg gtaataattt ctgctgaatt
    12601 gttgctaata gtaatatcga gtgtgagttc cttgtaataa tcgagaaacg agctttctga
    12661 tgcaatcatt ttccatgttc cattcatggc tgtatgctgc gaatagatga ttccggcaaa
    12721 cagaagaaaa gatacaaggc ttaatacaag ttttttcatc ggcttttaaa tttagcgtga
    12781 agatacaggt ttacattaag aaaaaaaacg cccagattga atattgaatc agtacttcaa
    12841 agtattgaaa atttaaaatt gaaataatta taaatccctt tacaataaag gaagttgttt
    12901 taatgcttcc aatctgcttt caactccggc ttacatgaat tcagttcctg aataagggag
    12961 tctccaaaga aataacttgt tttgtaatcg gtttccttac tactgcgtgg gtgcccattg
    13021 ccggttttta aatcgtacac agcaaagaca tgagtactat caaaaaaagt tacgaaaata
    13081 acgctgtcgc atgagcctgc tgtaaaatca gacaagtctt caatgtaatc actacaaccg
    13141 gttaataaag tcttttcaac aatcacctgt ccgtttccaa aaatgatttc ataatgtatg
    13201 cccgtagaat ggtcgaattc attatcctga tccagcttta catatatatt gttcagtcct
    13261 tcaacaggta agcggcctat atcgtgttga tcagtacagg aaagaattat tacgttgcac
    13321 aaaagaaaac agatgaatcc aattaaaaat aacttccttc tgctcattta ttttgttttt
    13381 cttaacaaaa atagaaaatt gttcaaataa aaattcaatc aaaaagcatg ggtataaatc
    13441 atttgttact ctgattattt tttcttaatt tgcatcatga tgaaattgaa aattttaagc
    13501 tctcttttgc ttttatgctt tttagtttct gggggtaagg cctgtgaagc gagaaagtta
    13561 tcatcaatac atctatttga gcagaagtgt ttagttttcg gcaaagaacc gattactggc
    13621 tacaatgtat ttcataaacg gctgccgcct ccatcgaaca atctaaaatt tgttaagcag
    13681 acattaccaa gggcaatcgt gagcttgttc atgcatggta gcgctgattt ccatatttac
    13741 tccccagttt acgaaatcgt tatttttctg caaccgcata caatcttctt gaagaatgtg
    13801 agtccggtta atgaaaaaag aggccctcct ttagactaac tcactttttt accccaaata
    13861 caccatacct gcaatgtata gaaccatggt tggaatggtt tgattgcaat aagcttgtat
    13921 atcattgcgt ctgtgtcatt cacaaactgc attacaaaca aattagtttt agtttaatta
    13981 aatgaaaaat tgtcatggaa aataaaagat atgatggaaa tcaaagttca ggaaaaaacg
    14041 aaaaagagtc gatggataaa acatctaaaa cgttgctcat actgtttata tgcattttta
    14101 tagccgctgt tataatcgtt cagttttttg tgtaaaaaag tgcgttaatc aaaaaattac
    14161 tgatgttgtg tattagagga acaagcacct gagatttaca atatataatc agtcgacaga
    14221 aagttagagt tcttagaatt caatatatca gtaagtatct ttttattcgt atcattctct
    14281 tttagtgcta cgagcgaacg aatagagaac acccggagtg catcgcttac cgataaggtg
    14341 ccttcggcag agtctttgcg tccggtaaat ggaaacacgt cgggacctcg ctgacattga
    14401 gaattaatat ttacacgaca taattgattt accagcgtgt cgacaagaac agatattatt
    14461 acggggtctt caccgaaaat gcttgcctgt tgtccaaaat ttgatgtaac gacatattct
    14521 aaaggttcct ccacatcgtg aaatttgaca accggaatca ctggaccaaa ctgttctaaa
    14581 tgataaatct gcattttgct gcttaccccg gaaagtacaa caggaaagaa aagtgatttt
    14641 tccataatac ccccattttt gttgcaaatt acagcaccat tattaacagc atcctcaatc
    14701 agatcctcca gccatttcgg tttataatct tctggcaatg gagtgatgtt tacatctttt
    14761 tcccatggca taccggattt cagagaatca acttcgctaa tgaaacggtt gataaaatca
    14821 tcgtaaatac tgtcgtgaac aaacagaatt ttgagtgcag tgcaccgttg tccattaaaa
    14881 gataaagcgc ccgtaacaca ttcttttaca gcgttttcaa tatttgcaac tttaagtatt
    14941 atagcagggt tcttggcttc cagccctaaa accgacttga gaagatgggg gtgagggtgc
    15001 tgttttttca aagtatcggc aaccttgcag gaaccaataa atgcaagtac atttaccttc
    15061 cccgattcca taatgggggc aattacttct cggccttcac catatactgt atttactacc
    15121 cctttcggga acgcttcctg aaaagctctg agcaacggag catgcagcaa tacaccaagt
    15181 tttgggggtt tgaatataac cgtgtttccc ataatcagcg ccggaatcaa cgtagcaaag
    15241 gtttcattca gaggataatt gaatggcccc atgcaaagaa ctacacccag aggtgatcgt
    15301 tttatctgtg caattacccc ctgagtgatt ttaagcctcg atgcttccct gtccatgtct
    15361 ttcagtgctt cgatggtatc gttgatatat tcaacggttc tgtcgaattc cttttgcgaa
    15421 tcggtaaggt tctttccgat ttcccacatc aatagtttta caattatatc acgttgttct
    15481 ttcatcagca aaatgaactt tctcatacac ccgatacgct gagaaatagt cattttaggc
    15541 caggttccct gtccattatt gtaggcttca atggcagcat gaagcgacat cattgctgtt
    15601 tttccatcca taagaggata ggagccaatc aagtttatca acgtgttatt ttcgtagaag
    15661 tacacagggg aaaatacttt ttcggttggt ccttcccaat gcatcaattc tccgtttaat
    15721 aaatactctg tttgattaaa aggtattgga atgcagtgtt cttcgggaat ttcctttaac
    15781 aaaggaaaaa ttttgttttg tatcataagc gataaaatgt attttcaaag atacaaatca
    15841 aaacccaata aaacacaagc tcattattgt tttgagaaag cactctaatg ttttaaaata
    15901 agttaaattt cgcacgccaa taaatcggta aaacactgta aaatgaaatc aaaattcttt
    15961 tcttgcttca ataacatgat aaaaacttgc tgtttagcta aaaaataaca ttattttcgc
    16021 caattaaaat ataaattcta ttttatggca gaaaacaata gtttattcgg ggaattcaaa
    16081 cccataagct acgaggaatg gcacgcacag gttataaagg acctgaaggg tgccgactac
    16141 gggcgcaagg tggtttggaa taccctcgaa ggcgtcagcc ttgaaccttt ctaccgtttc
    16201 gaggatgtca aagatctggc gtataccgat gcgctccccg gagaaaaacc ctttgttcgg
    16261 ggcagtaagc cttcgcacag ctggagaatc aggcaggact tccgtgctgc cgacccagca
    16321 gaagccaacc gcaaagcact gaaagcattg caaaaaggtg cgcaatcgct gggtttcgtt
    16381 acctgcactc cactcactta tcaacaactc gaaacattac ttaatggcat cgacctgacc
    16441 acagttccgg tgcatatagt aatgtttcga gttgttgata agtgagtgga gtgcaggtaa
    16501 c
//
LOCUS       DAOOQP010000066        16333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4329_length_16333_cov_5.889851, whole genome shotgun sequence.
ACCESSION   DAOOQP010000066 DAOOQP010000000
VERSION     DAOOQP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_16333_cov_5.889851"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2869
                     /locus_tag="PLK75_07935"
     CDS             <1..2869
                     /locus_tag="PLK75_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39215.1"
                     /translation="SATIDGSGCTNPVTSAGATVQVVADPSLSAATQTNSIICQGGST
                     IYSSNLTGGTGTVAYQWQYSADGITYSNVATGTPTGATYTNATSATMTIGGISAIGTY
                     YYQLYATIAGNGCTSPVTSASATVQVVADPSLTAASRTNSIICQGGSSVYSSSLSNGT
                     GTFTYQWQYSPNNSSWSNVITGTPAGAAYTNPTTTSMTVSGITVTSTYYYRLSATTTG
                     NGCTSPIYSLSASVQVVADPSDPSATKSPNTSNVCVGDILTLTGVTDNGGGTGTCNIE
                     YCYSTNAGNTYSSWSTSLPSFAATGTDNRIKIRKNCNGIGCDISPENTYIWSVDATSV
                     SGTASSNPASEICAGTTALLSLTGNTGNIQWQTNSSGTWQNIAGATNSTYTTPQLSKT
                     TSYRAIVTSGVCSSVTSNVIEVAVTIPSTSGILSNDYIWTGAINTAWDGNTNGNWIKF
                     SGSDFEVPASVPAEASNVFIRSNGSCFTAIPSVNTVNVAKCNKLTIDNGNSLSINSGG
                     NLTVNGDFTNSGTVTMSSSSTLYAKGNWVNDGTFNAGSGTVIFNGSSNQEIQSGASLF
                     NNVTFNNTATGVTDLNISESMIINGSAIFTNGVLYFTGTGSLSFTSNASSDIGTANSY
                     VDGQVTKSGTGAFTFPVGEGIVWAPVGIAAPSNNSTITAEYNFTEGPYNWSAAYMCDE
                     SQLHHTSGVEHWVLTTTNSTPAVTLYWYSGSRSGITDLTDLAVAHYTGSCWEYKAGSL
                     SGDLTSGTITSTISFSSYSPVSFGSKRGINPLPVELTSFDAKCTGTGVLLTWQTASES
                     NNSKFTIERSEDGSYWNIAASVDGAGNSITLKNYYYTDKIVSDSILYYRLSQTDFDGV
                     SKYSAVVSVFCKDAATEPGIFIYPNPFNNSLNIKVENWDSDVFIAEMYDVTGKKIGEW
                     QEANAPVDFTKEINIKGLAPGMYTIKITTAEKIIVRKVEKE"
     gene            2957..4378
                     /locus_tag="PLK75_07940"
     CDS             2957..4378
                     /locus_tag="PLK75_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOY39216.1"
                     /translation="MLYIIKLLFLCCLLIPVSVSAHKRALYNIIIDTDGGQDDFRAVT
                     LFCASPDFNINAITSTDGVLYPDETAGYFQLLLRGFGHEGIPVGVGEKNNCKKNFREH
                     ALLGWERLFPEYTAPLLPKSLQIMYDALANESKTTIIVALGPLTNISDILKSHPELKP
                     KIGLILWYNNDTELESGYNLESDIEAFQYLNASHIPLKIVGSISGFNYSSGFFEDLDS
                     LNNVYVAAFKKFNDAYGFENIEIWDDMCALYLGNINFFKETYNRSGLPAISPIEPFYG
                     DILAGSMLNTNKTGQGVVFSEIPVSDFWIMQDIRDEVDKIVQIHGVQEFKIVAMASEF
                     HSHLGAYSIVGAKMGFRALEYFHAGLDEIQVISYAGDLPPVSCMNDGLQYGTGATIGY
                     GNILVDTTNVRPSAKVIYNKRAIEISLKPEICHQIEADISQLVKKYGLESERYWAELR
                     MISIRYWHDWDRRDMFDIKILNE"
     gene            complement(4394..6268)
                     /locus_tag="PLK75_07945"
     CDS             complement(4394..6268)
                     /locus_tag="PLK75_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOY39217.1"
                     /translation="MFRNVIVLIILLGNASCIFSQPLTPVSVQIQMRDDKFLAADVYI
                     PQPQQPRPTILIQTPYNKNLYRWSLPLGFGVLPDLSPYNFVIVDWRGFYGSAAAFVAQ
                     YDRGLDGYDCIEWITQQSWSNGEIGTWGPSALGKIQFMTAKHQHPAHKCMVPLVASPE
                     YLYEEYYPGGVYREEYVEQLDALGYGMSTTLMAHPYYDFVWQYTQNQNNYPQLISIPC
                     LMISGWFDHDANMKIDYHKKLCNEAESSVRDKQWLIMGPWVHGGHGSSMVGSDQQGEL
                     SFPEAAGVSDVAALKFFGYYLLNAYNAWESTPKVQYFLMGENIWLDAEEWPPPGEIQV
                     ENYYLTETGELRKNIVLPSNSFSGFSYDPRDPSPTWGGPTLRNDLLQGPYDQVEMVES
                     RNDILVFTTSQLSEDVVVIGRPRVRLYVSSNCADTDIAVRLTDVYPDGSSILLLDDIF
                     RMRFLNGFSEQDVSFLQTGTIYEVEIEMSDIAICFQAGHYIRLDITGSNWPRFQRNKN
                     TDAAPFAAGDTLISNTRVYHQTNAASCIELPISFQNIIKDIPANSIDVYPNPANNRIY
                     LKSSSNCIIRNVELYDIQGEKRITSAENSIDISTVLPGIYFLKIFTDDGVIGKRFIKY
                     "
     gene            6449..6530
                     /locus_tag="PLK75_07950"
     tRNA            6449..6530
                     /locus_tag="PLK75_07950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6483..6485,aa:Leu,seq:tag)
     gene            6601..7947
                     /gene="tig"
                     /locus_tag="PLK75_07955"
     CDS             6601..7947
                     /gene="tig"
                     /locus_tag="PLK75_07955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOY39218.1"
                     /translation="MNITRQNTDDLNAVINVSITKDDISENVEKSLKEYKRKAKVPGF
                     RPGMVPMGLIKKMYGDALMADEINKKISEVLNKYLTDEKLNILGDPLPSDQQQPIDFA
                     TTDEYSFLFDIGLSPEFEVKLSKRDKINFYKIVVNEEMIEKQIQSHRQRFGSLIETDV
                     TDDKSMIKGSIVETDADFNPLENGIQKDDVSLYVGIIKDEEIKHNFVGTKAGSSIAFD
                     LKKALPSEAEISSMLGIDKKQVAAIGNFYRLTIDSIRTFTDAEMNKTLYEKATGNTEI
                     DTEEQFREAVKNELESALINESNYKFRIDAKNKLLSKCDLKLPEDFLRRWITFANKEV
                     TEEQLNKDFVHFMDDLRWTLIINKILKENSIEIQHDDVLEAAKDEILQQFRQYGIHQV
                     PDPTLIKYAEEMMHRESDAKRLFEKVGEAKALEFIKTAVKLEENLVSPEEFDKLFA"
     gene            7960..8643
                     /gene="clpP"
                     /locus_tag="PLK75_07960"
     CDS             7960..8643
                     /gene="clpP"
                     /locus_tag="PLK75_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOY39219.1"
                     /translation="MSTNNDFRKYATKHMGINSTTFDKITSVYNSYISPTIIEERQLN
                     IATMDVFSRLMMDRIIFLGLPIDDYVANIIQAQLLYLDSTDPQKDIQIYLNTPGGSVY
                     AGLGIYDTMQYIGCDVATICTGIAASMGAVLLCAGKKGKRTALKHSRVMIHQPMGGAE
                     GQASDIEITAREILKLKNELYEIISVHSGNPIEKVAADSDRDYWMIASEAKDYGMIDE
                     VLYRDNSAK"
     gene            8667..9884
                     /gene="clpX"
                     /locus_tag="PLK75_07965"
     CDS             8667..9884
                     /gene="clpX"
                     /locus_tag="PLK75_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOY39220.1"
                     /translation="MNTCSFCGRKEDEVNLLLSGIDAKICDDCVERAHEIISEEIKSS
                     KAKSFSKIELKKPAEIKAFLDEYVIGQEHAKKVLSVAVYNHYKRLNQSVQNNDVEIEK
                     SNIILTGETGTGKTLLARTISKLLKVPFTIVDATVLTEAGYVGEDIESLLTRLLQVAD
                     YDVAAAERGIVFIDELDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVNVPPQGGR
                     KHPEQKMIQVDTKNILFICGGAFEGIEKIISRRLNTQIVGYGAHKKSEGIDKTNLLRY
                     IAPQDLRAFGLIPEIVGRLPVLAYLEPLDRDALRSILTEPKNALVKQYIKLFEMDGVR
                     LTFTKDALEFVVDKAIEYKLGARGLRSICENLMLDAMYNLPGTKHKSFVVDLEFAKSK
                     LDAELMRRLKNAG"
     gene            10050..10646
                     /locus_tag="PLK75_07970"
     CDS             10050..10646
                     /locus_tag="PLK75_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39221.1"
                     /translation="MKQLFLIFLGLSMFLFAHSQNSQFPVNPDGAIEFTGSINTDLDV
                     KQFFEEAKLWATGNRHIVVYENTESGTITLKCLVKGKTSYNPFAGQFTESYDCVLKLT
                     HKGENVDYSISDIYIINNYMGYGMSTTTTKVEEKVAELEKTKADKSAAESNTELSKKK
                     RKDIIEDCDDAIATLTESLAVTKKFADDIIESLKVKFE"
     gene            10704..11738
                     /gene="rlmN"
                     /locus_tag="PLK75_07975"
     CDS             10704..11738
                     /gene="rlmN"
                     /locus_tag="PLK75_07975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOY39222.1"
                     /translation="MKTDIRKFEYPDIEEVITRFGESKFRAKQVYDWIWTKGAGSFEE
                     MTSLSKTLRSRLGDSFCFTRIQPLIQQVSRDGTIKTGFRLHDSHIVEGVLIPSNDRAT
                     ACISTQVGCAMNCHFCATGMLGFTRNLDHGEIFDQFVMLNALSIEKIGIPLSNMVVMG
                     MGEPFLNFENLMRALAIISSPEGINFSPTRITVSTIGIPEKIRAFADLHTTINLSVSL
                     HSAIDAVRSSMMPAAKKFKLSQIRDSIDYFYKNTGLRITYEYILFENINDDMKHAKAL
                     AEFCKISPCKINLIVYNHTSDSTFRASSSEHQSAFVEYLESKNLIVNIRRSKGSDIDA
                     ACGQLANKNR"
     gene            complement(11888..12217)
                     /gene="ssb"
                     /locus_tag="PLK75_07980"
     CDS             complement(11888..12217)
                     /gene="ssb"
                     /locus_tag="PLK75_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOY39223.1"
                     /translation="MNLRNRVQLIGNMGKNPIVKELNKGGKMARFSLATNEFYVKDGE
                     RVKETQWHNLVAWGKTADIISEHMAAGAEVVVNGKLKNRTYNDEKGNARYITEVVVDE
                     VLFRTTK"
     gene            12516..12662
                     /locus_tag="PLK75_07985"
     CDS             12516..12662
                     /locus_tag="PLK75_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39224.1"
                     /translation="MQLQETGSEKNSVQTMPTPDVTKMIIGAMEKNRLRVCVGQDFKF
                     LDFL"
     gene            complement(12721..12939)
                     /locus_tag="PLK75_07990"
     CDS             complement(12721..12939)
                     /locus_tag="PLK75_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39225.1"
                     /translation="MKLTYFTIIGILHTKIPKIIITLAIMYVYLSHNPTRGYHPIDYC
                     GIIASEKLTLMYQASDFIVLTSNKGKPG"
     gene            12960..>16333
                     /locus_tag="PLK75_07995"
     CDS             12960..>16333
                     /locus_tag="PLK75_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOY39226.1"
                     /translation="MRLRNNQLKIVNTFLLSVALGISSLIACNPPSNVSLSSILQESV
                     TFNWSCDDSAESWTIIYGPDGFIPALGGTIVSGISENQFTLSGLSPNTAYQVYIRSDC
                     SQEGNHSDLVGPFSFTTLITCPAPANIALISYTDSSTTFTWTQPDQTELWEIEYGPLG
                     FTLGTGTTITGITQNPFTITGLSYGTSYHVYIRAVCGELNSSWSQATGFTTNCIVLVP
                     DLSESFDTAPIPSKCWSVRSGQITTNSSLGYTNHWGSDGFANAGTTGAARVHVQGTAL
                     FGWLISPSINLGDGSVSYILEFDVALTSLHLTTNPQLTGTDDKFAVVISTDNGSTWSS
                     ANILRVWDNLGSPWVYNGLSPQGCHVTIDLSGYNGIIKIGFYAESTVSNASNDLFIDN
                     VAVNALPECMAPENVIAYDATETSVLVSWTDFTTTTAWDIIYGEFGFDPDVSGNIISG
                     IVANPFTLEGLVDGITYDIYVRAHCSGNYNNWTGPAQISTLPYCLSPIGLSYSEVSAS
                     SVDFSWNESGTANEWDIVYGQAGFDPFTGGTLISGLASTDFTIEGLLPSTEYNIYVRS
                     DCGEHKSNWSEKLKVITSCDNSTVLPVSESFESDLGYFINDFANNVDWDIISTVSTDG
                     SSCVRNHFSDNNSNSMILRCPIDLSTTINPVLTFAHMPLTFSDTDHCYVEILTNGSTW
                     TILPANRYMGSGNYHIPTQNNPEGPCFDMSSYYTSFSTPLTNAYWRNEKFSLSDYSGQ
                     AEVWLRFRVNSGSQLNFPGWYIDNIQIFDETCPKPADAEIIVIESSFAKLIWADNSFA
                     EQWKVIYGLHGFNPVGEGSVMISNDTMTVITDLIPESYYDVYVLGICSGDEQSEITGP
                     VSFHTTEQCVTPSELSVSNITKSQVTLAWAVPEPNSWIILYGETPLNIESGGILLSDI
                     SENPYTLTGLLPGIQYDVYVKADCDTDGESHWSGLYQFTMLPACLVPVGFTVSDVTAS
                     TALVSCANEPDATGWAILYGVSGFNPYTDGTIISGINTADYTISGLTPHTSYDIYCRS
                     ICGSCHSEWSGKIEINTTCYEPFNLPLIETFENGFGAFINSEGNGANWSIESAIAFEG
                     QHCIKNAYTNSDNNLIVLRCPVDLSYAQHPYISFAS"
BASE COUNT         4937 a         3383 c         3393 g         4620 t
ORIGIN      
        1 atcagccaca attgacggca gtggctgtac aaaccctgtt accagtgccg gagcaactgt
       61 gcaggtggtt gcagatcctt cgttatcggc tgctacacaa accaattcta ttatctgcca
      121 gggaggttca actatataca gcagcaatct taccggtggt acaggcactg ttgcatatca
      181 atggcagtac tctgccgatg gaattactta tagcaatgtg gctacaggaa cacccaccgg
      241 agctacatat accaatgcga catcggccac aatgactatt ggaggtattt cagccattgg
      301 cacatactat tatcagttgt atgctacaat tgcaggaaat ggttgtacaa gtcctgtcac
      361 aagcgcttca gcaactgttc aggtggttgc agacccgtcg ttaacagcgg catcgcgtac
      421 caattcaatc atttgccagg gtggttctag cgtttatagt agttcattgt caaatggaac
      481 cgggacattt acttatcaat ggcaatattc accgaataat tcctcatgga gtaatgttat
      541 tacaggaaca cctgccggag ctgcttatac aaacccaact acaaccagta tgacagtaag
      601 tggtatcaca gtaacttcta cctattatta caggctttct gctactacca ccggtaacgg
      661 ttgcaccagt cctatctaca gcttaagtgc cagtgttcag gtggttgcag atccatccga
      721 tccctcagct actaagtctc ccaacacttc taatgtttgc gttggtgaca tacttacact
      781 taccggggta accgataatg gtggtggaac cggaacatgc aatatagagt attgctattc
      841 gacaaatgca ggtaacacat attcatcatg gagcacctcg ctacccagct tcgcagcaac
      901 cggcaccgat aacagaataa aaattcgtaa aaattgtaat ggcatcgggt gcgacatcag
      961 tccggaaaac acctatatct ggagtgttga tgcgacttcg gtaagcggaa cagcttcatc
     1021 aaatccggct tcggaaattt gtgccggaac tactgctttg ctgtctctca cgggtaatac
     1081 aggaaatatt caatggcaaa ctaattccag tggaacatgg cagaatattg caggagcaac
     1141 caattcgacc tatactacgc cacaactttc taaaaccaca tcgtaccgtg caattgttac
     1201 cagtggagtg tgcagttcag tcacttccaa tgtgattgaa gtggccgtta caattccatc
     1261 aacctcaggt attcttagca atgattatat atggacagga gcaattaata cagcctggga
     1321 cggaaatacc aatggtaatt ggattaaatt ttcaggctcc gattttgaag ttccggcttc
     1381 tgtacccgca gaggcttcaa acgtatttat tcgttctaat ggaagctgtt ttacagccat
     1441 accatcggta aacactgtga acgttgcaaa atgtaataaa cttaccattg ataacggaaa
     1501 cagcttaagc attaattccg gaggaaacct tacagtgaac ggagacttca caaattcagg
     1561 aactgttact atgagcagtt catcaacgct ttatgcaaaa gggaactggg ttaatgacgg
     1621 tacatttaat gccggttcag gaactgtgat attcaacggt tcatcgaatc aggaaattca
     1681 atcaggagca agcctcttca ataatgttac atttaacaat actgcaaccg gagttacaga
     1741 tttgaatatc agtgaatcaa tgattataaa cggatctgct atatttacga acggagtgct
     1801 gtattttaca ggcaccggat cgctgtcatt tacttcaaat gcttcatcgg atattggaac
     1861 ggcaaacagc tatgttgacg gtcaggtcac caaatcaggt acgggtgcat tcacatttcc
     1921 ggttggagaa ggtattgtat gggcacctgt aggaattgcg gcgccttcga ataattcaac
     1981 aattactgcg gaatataatt tcacagaagg accttataac tggtcggcag catatatgtg
     2041 cgacgaaagc cagttgcacc atacaagcgg ggtagaacac tgggtactga ctaccacaaa
     2101 ttcaactccc gctgtaactt tatactggta tagcggcagc agaagcggta ttaccgatct
     2161 gaccgacctt gctgttgctc actatacagg tagttgctgg gagtacaagg caggttcact
     2221 ttcaggggat ctgacttcgg gtacaataac ttctaccata tcattttcat cttacagtcc
     2281 ggtaagcttc ggaagtaaac gcggtattaa tccgctgcct gttgagctta caagttttga
     2341 tgcaaaatgt actggaactg gtgtactact tacatggcaa actgcatcgg aaagcaataa
     2401 ttcaaagttc accatcgaac gaagcgaaga cgggtcctat tggaatattg cagcatctgt
     2461 tgatggggcc ggcaactcaa tcacgctcaa gaactactat tataccgata aaattgtttc
     2521 tgactcaata ctgtattatc gtttaagcca gactgatttt gacggagtaa gtaaatattc
     2581 ggctgtggta agtgtattct gtaaagatgc tgccacagag ccgggtatat ttatttaccc
     2641 taatccattc aataacagct tgaacattaa agttgaaaac tgggacagcg atgtattcat
     2701 tgctgaaatg tacgatgtaa ccggcaagaa aatcggagaa tggcaagaag caaatgcacc
     2761 tgttgacttc acaaaagaaa tcaacattaa gggactggca cccgggatgt acacaatcaa
     2821 aataacaacg gctgagaaga ttattgtgcg caaggtagaa aaagaatagc cggaatttga
     2881 ttaacatatc aatttataat ttccggattt taaaccggat aataataatt gtatttttgc
     2941 acaaaacagt aaacccttgt tgtatataat aaaactactc tttctgtgtt gcttactcat
     3001 tccggtaagt gtttcggcgc acaaacgcgc tttatacaat attataatcg acaccgatgg
     3061 cggacaggat gattttcgtg cagtaacgtt attctgtgca tctcccgatt tcaatattaa
     3121 tgcaattact tctaccgatg gggtgcttta ccccgatgaa acagccggat attttcagct
     3181 tctgctgaga ggttttggcc atgagggaat acctgttgga gtaggggaaa aaaataattg
     3241 caaaaaaaat ttcagagaac atgcactttt agggtgggag cgacttttcc cggaatatac
     3301 tgctcctctc ctaccaaaat cgttgcaaat catgtatgat gcccttgcaa acgaaagcaa
     3361 aacaacaata atagttgcac tcgggcctct aaccaatatt tcagatattt taaaaagcca
     3421 cccggagctc aagcctaaaa tcggactaat actttggtac aacaacgata cagagttaga
     3481 atcaggttac aatcttgaat cggacattga agctttccaa tatcttaatg ccagccacat
     3541 cccactcaaa attgtcggat ctatctcagg ttttaactat tcttccgggt ttttcgaaga
     3601 cctcgatagt ttaaacaatg tctatgttgc tgcttttaag aaattcaatg atgcctacgg
     3661 gtttgagaac attgagatat gggacgatat gtgcgccttg tacctcggta acattaactt
     3721 ctttaaagaa acatacaacc ggtcgggttt gccagcaatc agccctattg aaccatttta
     3781 cggtgatata cttgccggat cgatgcttaa tactaataaa accggacagg gagttgtctt
     3841 tagcgaaata ccggtttcgg acttctggat tatgcaggac atacgggatg aggtagataa
     3901 aattgttcag attcatgggg ttcaggagtt taaaattgtg gcaatggcat ctgagtttca
     3961 ttctcacctg ggagcctata gtatagtcgg agcaaagatg ggattcagag cactggaata
     4021 ttttcatgca gggctcgatg aaattcaggt aatttcgtat gcaggggatt tacctcctgt
     4081 cagttgtatg aacgatggac tccaatacgg cacaggcgca accatagggt atggaaatat
     4141 tcttgtcgac accaccaatg taaggccttc agccaaagtt atttataaca aaagagctat
     4201 tgaaattagc ttaaaacccg aaatatgcca tcaaatagaa gctgatattt cgcaattggt
     4261 taaaaaatac ggtcttgagt cggaaagata ctgggctgag ttacgcatga tttccattcg
     4321 ttactggcac gattgggatc gtagagatat gttcgatata aaaattttga acgaataaaa
     4381 tcttcagcga tttttaatat ttaataaagc gctttccaat cactccatca tcagtaaaaa
     4441 tcttcaggaa gtatatcccc ggcaatactg ttgaaatgtc aattgaattt tcagcagatg
     4501 taattcgttt ttcaccctga atatcgtata gctcaacatt tcttattata caattactgg
     4561 aactttttag gtaaattcta ttatttgcag ggttcggata cacatcaata ctatttgcag
     4621 gtatgtcttt aataatattt tggaagctga taggtagctc aatacaggag gctgcattgg
     4681 tttggtgata aacccgggta tttgatatca gcgtatcgcc tgctgcaaaa ggggctgcat
     4741 cagtattttt atttcgttga aagcggggcc agttgctgcc ggttatatca aggcggatat
     4801 aatgccctgc ctgaaaacaa atagcaatat cactcatttc gatttcaact tcgtagatag
     4861 tcccagtctg caaaaaactc acatcctgct ctgaaaatcc attaagaaac cgcattctga
     4921 atatatcatc gaggagtaat attgaacttc cgtcggggta aacatctgtc agccttacag
     4981 caatatcggt atcagcgcag tttgaagaaa cgtacagtct tacacgtggg cgtccgataa
     5041 caacaacatc ttcggaaagt tgtgaagttg taaaaaccaa tatgtcgttg cgcgattcca
     5101 ccatttccac ctgatcgtaa ggtccctgta ataaatcatt tcgaagcgtc gggccacccc
     5161 atgtaggaga agggtcacgt gggtcgtacg agaaccccga gaacgagttc gaaggtaaaa
     5221 caatattttt tctgagttct ccggtttcgg taagataata attctccact tgtatttcgc
     5281 ccggaggagg ccattcttct gcatcaagcc atatgttttc acccattaaa aaatattgca
     5341 ctttaggtgt tgattcccat gcgttgtagg cattgagcaa ataataacca aaaaatttca
     5401 atgctgcaac atccgaaact cctgccgctt ccggaaatga taactccccc tgttggtcgc
     5461 tgcctaccat tgagctgccg tgcccgccat gcacccatgg acccattatg agccactgct
     5521 tgtcgcgaac agaagattct gcttcgttgc atagtttttt atgataatcg attttcatgt
     5581 ttgcatcatg gtcgaaccag ccgctgatca tcagacacgg aatgcttatt aattgggggt
     5641 aattgttctg gttttgagta tattgccata caaaatcgta ataaggatga gccatgagcg
     5701 ttgtcgacat gccataaccc aaagcgtcga gttgctcaac atactcttca cgatatacgc
     5761 ctcccgggta atattcttcg taaagatatt caggagaggc gacaagcggc accatacatt
     5821 tatgagcagg gtgctgatgt ttagctgtca tgaattgtat tttccccaat gcagatggtc
     5881 cccatgtacc tatctcacca tttgaccagc tttgttgtgt tatccattcg atacagtcgt
     5941 acccatctaa acctcggtcg tactgcgcca caaaagcggc cgctgagcca taaaagccgc
     6001 gccaatctac aattacaaaa ttatatggcg ataaatcggg caagactcca aaccccaagg
     6061 gtaaactcca gcgatacagg ttcttattat aaggggtttg aataagaatg gttggcctag
     6121 gttgttgtgg ttgtggaata taaacatctg ctgcgagaaa tttatcatcg cgcatctgta
     6181 tctgaacact aaccggggta agaggctgcg aaaatataca gcttgcattg ccaagcaaga
     6241 taattaacac aatgacattt ctaaacataa aagagttttc atcaaaatta aaaaaacaaa
     6301 tcgatgtttt tctgaatttt gattatcaaa caatatacat aaaaccagcc ctgatatgta
     6361 tgcaagaata cttaaattta aggaatttta tcttttaggt aaaacataga aaaaatttga
     6421 aaaataaatt acctttgcag cgatttttgc ggatgtggcg gaattggtat acgcgccaga
     6481 cttaggatct ggtgccgcaa ggcttggggg ttcgagtccc ttcatccgca caacaataat
     6541 tctgcttcaa tactcaggca ttggggcagt tttattcatt atttgtataa atattaactt
     6601 atgaacatta cacgccaaaa caccgatgat ttaaatgctg ttatcaatgt aagcattaca
     6661 aaagatgata tttcggaaaa cgtagaaaaa tcgctaaaag aatataagcg caaagcaaaa
     6721 gttcctggat tcagaccggg aatggttccg atgggtttga taaaaaaaat gtatggagat
     6781 gcattgatgg ctgatgaaat caataaaaag atttctgaag tacttaataa gtaccttacg
     6841 gatgaaaagc tgaatattct gggggacccc cttcccagcg atcagcaaca acctattgac
     6901 tttgctacta ccgatgaata ttcgtttctt ttcgatattg gcttgtctcc ggagtttgaa
     6961 gttaaacttt cgaaacgcga caagatcaat ttttataaaa tagtcgtaaa cgaagaaatg
     7021 atcgaaaagc agatacaaag tcaccggcag cgttttgggt cattaattga aaccgacgta
     7081 accgacgata agagcatgat caaaggatcg atagtggaaa cggacgccga tttcaatcca
     7141 ttggaaaacg gtattcagaa agacgatgta agtctttatg tcggcataat caaagatgag
     7201 gaaattaaac acaatttcgt cggcaccaaa gcaggcagca gtatcgcatt tgatctgaaa
     7261 aaagccctcc cgagtgaggc tgaaatctcg tcgatgcttg gcattgataa aaagcaagtt
     7321 gccgctatcg ggaatttcta ccgacttacc atcgacagca ttcgcacatt caccgatgca
     7381 gaaatgaaca agacccttta tgaaaaggca accggcaata ctgaaattga taccgaagag
     7441 caattcagag aagcagtgaa gaacgaactc gaatcagctt taatcaatga aagcaattat
     7501 aaattcagaa tcgatgcaaa aaacaaactt ctgtcgaaat gtgatcttaa acttccggaa
     7561 gatttcctgc gcaggtggat tacttttgct aacaaagagg ttactgagga gcaacttaat
     7621 aaagacttcg ttcattttat ggacgactta aggtggacac ttatcataaa taaaatcctg
     7681 aaggaaaata gcatcgaaat tcaacacgac gatgtgcttg aggctgctaa agatgaaata
     7741 ctacaacaat tcaggcaata tggcattcat caggttccgg acccaaccct tataaaatac
     7801 gccgaagaaa tgatgcatcg cgaaagtgat gcaaaacgtc tgtttgaaaa agtgggtgag
     7861 gcaaaagcac ttgagttcat taaaactgca gtaaaactgg aggaaaattt ggtaagtccc
     7921 gaagaatttg ataaattgtt tgcctaaaac ctgattatca tgtcaacaaa caacgatttc
     7981 cgcaagtatg ccaccaagca catgggcatc aacagtacca cattcgataa aatcacctcg
     8041 gtgtataaca gctatatttc accgaccatc atcgaagaac gtcaactgaa tattgctact
     8101 atggatgtct tctcccgctt gatgatggac agaataattt ttcttggtct gccaatcgat
     8161 gattatgtgg caaacatcat tcaggcccaa ctactgtatc ttgattctac cgatccgcaa
     8221 aaagacattc agatttatct gaacactccc ggagggtcag tttatgcagg tcttggaatt
     8281 tatgacacaa tgcagtatat cggttgcgat gtggctacca tttgcacagg tattgcagcc
     8341 tcgatgggtg ccgttttact ttgtgccgga aagaaaggga agcgaacagc gttaaaacat
     8401 agtagagtaa tgatacacca gcccatgggc ggcgctgaag gtcaggcctc tgatatcgaa
     8461 attacagcac gggaaatatt aaaacttaaa aacgaattgt acgaaatcat ttctgtacat
     8521 tcaggcaacc ctattgaaaa agtggccgcc gattccgacc gcgactactg gatgattgca
     8581 tctgaagcaa aagactacgg tatgattgat gaagttttat accgcgataa ttcagcaaaa
     8641 taattacata tcattacttt taaataatga atacctgctc attttgcgga agaaaagaag
     8701 atgaagtaaa tttgctctta agcggcattg atgctaaaat ctgtgatgac tgtgtagaaa
     8761 gagcgcatga aattatttct gaagaaataa aatcgtcgaa ggccaaatca ttctcgaaaa
     8821 tagaattaaa aaaaccagct gaaattaaag cattcctcga tgaatatgta atcggtcagg
     8881 aacatgccaa gaaagtgctt tcggttgcgg tgtacaatca ctacaaacga ttgaatcaat
     8941 ctgttcagaa caacgatgtt gagattgaaa aatcgaatat aatactcact ggcgaaaccg
     9001 ggaccggtaa aaccctgctc gcccgcacca tttcgaaact gctgaaggta ccatttacca
     9061 tcgttgatgc cacagtactc accgaagcgg gttacgtggg tgaagacatc gaaagtttgc
     9121 tgacgcgtct gcttcaggtc gccgattacg atgtggctgc tgccgagaga ggcattgttt
     9181 ttattgatga acttgataaa attgcccgca agggcgacaa cccaagtatt acgcgcgacg
     9241 tttctggaga aggtgtgcaa caaggtttat taaagcttct cgaaggctct gtcgtaaacg
     9301 taccccctca gggtgggcgc aaacatccag agcaaaaaat gatacaggtc gatacaaaaa
     9361 atatcctttt catttgcggt ggggctttcg aaggtatcga aaaaattatt tcccgaaggc
     9421 ttaacaccca gattgtggga tatggtgcac acaaaaaatc ggaaggcatc gacaagacca
     9481 atctactccg ttatattgcc ccacaagatt tacgggcatt cggactaata cctgaaatcg
     9541 tgggaagact tcctgttctg gcgtatttgg agcctctcga cagagatgcc ttgcgaagca
     9601 ttcttacaga acccaaaaac gctttagtaa agcaatatat taagcttttc gaaatggatg
     9661 gcgttcgcct gacattcacc aaagatgccc tggaatttgt tgttgacaaa gctatcgaat
     9721 acaaacttgg cgcaagggga ctgcgatcaa tatgcgaaaa cctgatgctc gatgccatgt
     9781 acaatttacc cggcactaaa cataagagct ttgtcgttga tcttgagttt gccaaaagta
     9841 agctggatgc tgaactcatg cgtcggttaa aaaatgctgg ttagctgcaa tatattcttg
     9901 gcaagaaatc cattcttctt aaattttaat gccaccgtag aagtaataca catacatggc
     9961 taacaatatt ttcatatttt tgtgatattg cattcaaaaa ttcacatctt gtaatttacg
    10021 ttcttattta catagttaaa caaattatta tgaaacaatt atttcttatt tttcttggat
    10081 tgtcaatgtt tctatttgct cacagtcaga acagccaatt ccctgtaaac cccgatggag
    10141 caatcgaatt taccggttcc atcaacacgg atcttgatgt gaaacagttc ttcgaagaag
    10201 ccaaattatg ggctacaggt aatcgacaca ttgttgttta cgaaaacaca gaatcaggta
    10261 ccattacatt aaaatgcctg gtaaaaggta aaacttcata caatccattt gctggccagt
    10321 ttaccgaatc gtacgactgc gttctgaaac tcacccataa aggggaaaac gttgattatt
    10381 ctatttccga catttatatc attaataact atatgggtta tggcatgagt acaaccacaa
    10441 caaaagttga agaaaaagtg gctgaacttg aaaaaacaaa agccgacaaa tcggctgccg
    10501 aaagcaatac agaactatcg aagaaaaaac gcaaagatat aatcgaagat tgcgatgacg
    10561 ctattgccac attaactgag tcgctagctg taacaaaaaa atttgcagat gacattatcg
    10621 aaagcctgaa agtaaaattt gaataaatat cagttggttt gctgatatgc ctttttcgtc
    10681 gtattgataa ataagattat tttttgaaaa ctgacatccg gaaatttgaa tatcccgaca
    10741 ttgaagaagt aattacacgc tttggagaaa gtaaattcag agctaagcaa gtttatgatt
    10801 ggatttggac aaaaggggcc ggaagtttcg aagaaatgac atccctttca aaaacgctcc
    10861 gcagccgttt gggcgacagc ttctgcttta cgcgtattca accgttaatt cagcaagtca
    10921 gcagggatgg aacaataaaa acagggttcc gcctccatga ctctcatatt gttgaaggag
    10981 tgctgattcc ctcaaacgac cgggctacag cctgcatttc gacacaagta ggatgcgcca
    11041 tgaactgtca tttctgtgct accggtatgc tggggtttac ccgcaatctg gaccatggag
    11101 aaatattcga ccagtttgtg atgctaaatg ctctcagtat cgaaaaaatc ggaatacccc
    11161 tgtcgaacat ggtggtgatg ggcatgggag aaccattttt gaatttcgaa aacctaatgc
    11221 gcgcactcgc tattatttca tcccccgaag gaataaactt ctctccgacc cgcattacgg
    11281 tttcgacaat tgggattccc gaaaaaataa gggcgtttgc cgatttgcat actacaatta
    11341 atctgtctgt atcgctgcat tctgctattg atgcagtacg aagcagcatg atgcccgcag
    11401 ccaagaaatt caaattgtct caaattcgcg acagcattga ttatttctac aaaaataccg
    11461 ggctcagaat tacctacgaa tatattttgt tcgagaatat taacgacgat atgaaacacg
    11521 ccaaagcact tgcagaattt tgtaaaatat cgccatgcaa gataaacctg attgtgtaca
    11581 accacacttc tgatagcact ttcagggctt catcgtctga acatcaaagc gcttttgttg
    11641 aatatcttga gagcaaaaat cttattgtaa acattcgccg gtcgaagggg tccgatattg
    11701 atgcagcctg cggtcagctt gccaataaaa accgttgatt tcttaatttt tcaaaacaaa
    11761 ctcctgcata ggacaataaa aaaaccggtg cctgaatcac ttcggtttcg gaaagcattt
    11821 atgcactcct actcactggg tatattcctg caaccggtgt ttacgctgca aagtttatat
    11881 gccgcgtcta tttggtcgta cggaacagca cttcgtcaac caccacttcg gtaatatagc
    11941 gggcattgcc tttttcatcg ttgtaggtgc gatttttaag ttttccgttc accaccacct
    12001 ctgcacctgc agccatgtgt tccgaaatga tatcggctgt ttttccccat gccacaagat
    12061 tgtgccactg cgtttctttc acacgctcac cgtctttcac gtagaactcg tttgttgcca
    12121 gcgagaacct tgccatttta ccacctttgt tcaactcttt tacaataggg tttttaccca
    12181 tgtttccgat caactgtacg cgatttctta aattcataac actaaaaatt aaattaaaca
    12241 tttctttatt tcgcaatgcc gactgtgttg atgtcaatcg acgatgcaaa gatgcagcag
    12301 cggtttttcc ggtttcggtt ttttgttgtt tgctttcgct tattattcgt ttgttgtcgg
    12361 ttgctgtcgt tttaatactt gttttacaat tacttatatc attttttaaa atattttatt
    12421 attattccga aatacggggc atcgatgtac aggttaccgt tatattcccc agatccattg
    12481 ccacatacat ttcagtaaac tcagggctca cagttatgca gttacaagaa accgggtcgg
    12541 agaaaaatag cgtgcagaca atgccaactc ccgatgtaac aaaaatgatc attggtgcca
    12601 tggagaaaaa tcgtttgcgt gtttgtgtgg gtcaggactt taaatttctc gactttctgt
    12661 aacgcatgtt cccggagtat gccaacggct ttattgaaaa aaatggatgc actgctgaac
    12721 ttaaccaggc ttgcccttat tgcttgtcaa tacaataaaa tcggaagcct gatacatgag
    12781 cgtcagtttt tcactagcta ttattccaca gtaatcaatt gggtgatatc ctcttgtcgg
    12841 gttatggctt aaatacacat acataattgc cagggttata ataatttttg gtatttttgt
    12901 atgtagaata cctattatcg taaaataagt taatttcaaa ctgaataaat tttaataaaa
    12961 tgcgcctgag aaacaatcag ttgaaaattg tgaatacgtt tctgctctca gtcgctttag
    13021 gaatttcttc gctaatagca tgtaaccctc cgtcaaatgt cagcctttcg agcatcttgc
    13081 aggaaagtgt tacttttaat tggagttgtg atgactctgc tgaatcttgg acaataatat
    13141 atggccccga tggttttatt cctgcactag gaggaaccat tgtttctggc atttcagaaa
    13201 atcaatttac actatcaggt ttatcgccca atactgccta tcaggtttat attcgttccg
    13261 attgcagtca ggaaggtaat catagcgatt tagttggccc ttttagtttc acaaccctga
    13321 taacatgtcc ggccccagcg aatatcgctc taatttcata taccgattcc agcacaacat
    13381 tcacctggac tcagcccgat caaactgaat tatgggaaat tgagtacggt ccgttgggat
    13441 ttactttagg tactggaaca accataaccg gcattactca aaacccattc acaataactg
    13501 gtttgtcgta tgggacaagc taccatgtgt acatcagggc tgtatgtggc gaattaaaca
    13561 gttcttggtc gcaggcaact ggttttacaa caaattgcat agttttagta cctgatttat
    13621 cggaaagttt tgataccgcg ccaattcctt cgaaatgctg gagtgtcagg tcgggacaaa
    13681 taaccaccaa ttcttctctg ggttatacaa atcactgggg ctctgatggc tttgcaaatg
    13741 ctggaacaac cggtgctgca agagtacacg ttcagggcac agcgttgttt gggtggctga
    13801 tatcgccatc aatcaatctt ggggacggct cagtttcgta tatacttgag tttgatgtgg
    13861 cattaacaag cttacacctt acaacaaacc ctcagctaac aggcaccgac gacaaatttg
    13921 cggttgtgat ttcgacagat aacgggtcaa catggagttc tgcaaacatt ctcagggtct
    13981 gggataatct gggttcaccc tgggtttaca atgggctaag ccctcaggga tgtcacgtga
    14041 ctattgacct ttctggttat aacggcataa taaagattgg cttttatgcg gaatcgactg
    14101 tttcgaatgc aagcaacgat ctgtttatcg acaatgtcgc tgtaaatgct ttgccggaat
    14161 gcatggcacc tgagaatgtt attgcatacg atgccactga aacatccgta ttggttagct
    14221 ggaccgattt tacaacaacc actgcatggg acattattta cggtgaattt ggttttgacc
    14281 ctgatgttag tggtaacatt atatccggga tagttgcaaa tccgtttacc ctcgaagggt
    14341 tagtcgatgg aataacttat gatatttacg tacgagccca ttgttccggc aattacaaca
    14401 actggacagg cccggcacag ataagtaccc tgccctattg cctgtctccc atcggtctaa
    14461 gttactccga ggtttcggca agctccgttg atttcagctg gaacgaatca ggcactgcaa
    14521 atgaatggga tatcgtttat gggcaagcgg gatttgatcc tttcaccggg ggaactctta
    14581 tttcaggcct tgcttcgact gatttcacca tcgaagggct attaccctca acagagtata
    14641 atatttacgt taggtccgac tgtggtgagc acaaaagtaa ctggtcagaa aaactcaaag
    14701 tcatcacttc atgcgataat agtacggtct tacctgtttc tgaaagtttt gaatctgacc
    14761 tgggatattt cattaacgat ttcgcaaata atgtcgactg ggatattata tctactgttt
    14821 caaccgacgg atcgagttgc gttagaaacc attttagcga caacaactcg aacagcatga
    14881 ttttgcgttg tccgattgac ttgagtacaa ccataaaccc ggttctaaca tttgctcata
    14941 tgccactaac attttccgat accgaccatt gttatgttga aatattaacc aatggtagta
    15001 cttggacaat tctacccgcc aaccgatata tgggctctgg caactatcat attccaacac
    15061 aaaacaaccc tgaaggccca tgttttgata tgagttcata ttatacatca ttttcgactc
    15121 cattgaccaa tgcgtattgg aggaacgaaa agttttcact ttccgattac tcggggcagg
    15181 ctgaagtctg gctgaggttt cgtgtcaatt caggttcaca gcttaatttc cccgggtggt
    15241 atatcgacaa catacagatt ttcgacgaaa cctgccccaa acctgccgat gcagaaatta
    15301 tagttatcga aagcagtttt gcaaaattaa tttgggcaga caatagcttt gcggaacaat
    15361 ggaaagttat ttatggcctt catggtttta atccggtagg tgaaggttct gttatgattt
    15421 caaatgacac aatgacggtg attacagact tgattcctga aagctattac gatgtttatg
    15481 ttttgggtat ttgctcaggc gatgagcaaa gtgagatcac ggggcccgtt tcatttcata
    15541 ctaccgaaca atgtgtaacg ccttcggagt tgtcggtatc aaacattaca aaatctcagg
    15601 taacgttagc atgggcagtt cctgaaccca attcctggat aattctttac ggagaaactc
    15661 ctctgaacat tgaatccgga gggatactgc tatcggatat ttcagagaac ccatacaccc
    15721 tgacaggcct tctccccggc atacaatacg atgtatatgt aaaggctgat tgcgatacag
    15781 atggagaatc gcactggtcg gggctttacc aatttaccat gttaccggca tgtttggttc
    15841 cggttggttt tacagtttcg gatgtgaccg caagcacagc tcttgtttcc tgtgctaacg
    15901 aacccgatgc aaccggatgg gcaatattat acggcgtctc tgggtttaac ccttataccg
    15961 atggaactat aatcagtggt attaacaccg ctgattatac aatttccggg ttaacgccac
    16021 acacatcata cgatatatat tgcaggagta tttgcggaag ctgccatagc gaatggtccg
    16081 gtaaaattga aataaatacc acatgctatg aaccgttcaa ccttccatta attgaaacct
    16141 tcgaaaatgg atttggcgcg tttatcaatt ccgagggaaa cggggcaaac tggagcattg
    16201 aatctgcaat agctttcgag ggtcagcatt gtattaaaaa tgcatacacc aattccgata
    16261 ataacctgat tgtgttacga tgccctgttg atttaagcta tgcacagcat ccatatatta
    16321 gttttgcaag cat
//
LOCUS       DAOOQP010000067        16304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4337_length_16304_cov_6.481999, whole genome shotgun sequence.
ACCESSION   DAOOQP010000067 DAOOQP010000000
VERSION     DAOOQP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4337_length_16304_cov_6.481999"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLK75_08000"
     CDS             complement(<1..988)
                     /locus_tag="PLK75_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39227.1"
                     /translation="MRRLRKFVFASIAVCLTINAMADKDPRIPSQNNDNQKNTRAAAA
                     CEPSKTSTELYINNVRALIHTGGDMWWDLQGDPRYEVPAGSGRNALFAGSIWIGGKDA
                     NGQLKLAAQRFRQVGIDFWPGPLIVSGNLQGTTSQEICREYDKHFVISKEMVKEFREW
                     YACSQDPDCDVAENFSTYTIPDIILNWPAHGPSGGYDYYLAPFWDVDGDGTYNPLQGD
                     FPYYEFANEGITDDPDCMRPRDRMSKLFGDYTLWWVYNDRGNIHTETGGDPIGMELRA
                     QAFAFTTNDELNDMTFYNYNIINRSTYTLYKTYFGVWTDADLGGATDDYTGCD"
     gene            complement(1023..4658)
                     /locus_tag="PLK75_08005"
     CDS             complement(1023..4658)
                     /locus_tag="PLK75_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY39228.1"
                     /translation="MLQRLLLLTVAMAFYASIFAQSGTFSGTVKDAATGEPIPFANVS
                     VEENGRIVTGGMTDFDGKFSIKPIPAGKYTVKASYVGYATLQYNNVQIPAGKITFQDF
                     DLKASAEILQEVEIKDYKVPLIAKDQTESGGTVTSEDIAKMPGRSATAVATTVAGVYS
                     EDGEVGSIRGARSEGTVYYVDGVKVRGSSSIPKSAIEQVTVITGGLAAKYGDATGGII
                     SVTTKGPTSKYYGGAEFLTSQFLDPYGYNLGGVMLSGPIWTIEEKGTNRKRTVAGFLI
                     SGEVNNIIDDYPSAVGVWRAKPEVVESIRTNPLRLVQTENGAIIYQNADYLGADAFTN
                     EKTRPDAENLQVNLAGKIDFQPVRNLNLTFGGTMDYRLGRAYSRSNDLFNSSNNGAYA
                     YNNWRVYGRLTQKFDSGKSDEEEDNSVIRNAYYTIQVDYSKTYSNYFDRTYRDNFFQY
                     GHVGTFQTDVLKFYNYGVDTVANLEGWLQEVFVYAIDTFIPSTYNEDLAIYSTQFFDY
                     FSDYSLLRKEDVELNGGLLNGQSAPSVYGLWASPGNPYTSYTISDATQFRVSASGTAD
                     IKDHEISFGFEFEQRNDSYYGLAPFGLWSLARKLMNYHINEKDLANPIIERDANGVYL
                     DTINYPRLYNAETQSTFDKNFRTYLGYDITSLNWIDIDSYDPDQFDISYFSADELFNQ
                     GNNYVAYYGYDHTGKKLNNNATIEDFFTQTDANGVLTRPIPSFQPNYIAGYIQDKFAF
                     KDLIFNIGLRVDRYDANQKVLKDQFLFSEAYTVGDNDPQGLIAGVEHPSNIPDNAVVY
                     VNDLNNPSGINGYRVGNVWYDAAGTEVSNPNRIASANGIAPYLIDKETELSAGAFEDY
                     KPQLVFMPRISFSFPISDEALFFAHYDILSKRPTYYNRLDLIEYYFIRQKNGDILNNP
                     NLKTEKTIDYELGFQQKLGNTSSLKLSAFYREMRDMQQAVAVYGAYPVNYYTYGNIDF
                     GTVKGFTASYDLRRTGNVTMRVAYTLQFANGTGSNVESAAAIIKSDQPNLRTSIPLDF
                     DQRHNIAITVDYRFGGKVSGTPYDGPRWFGADIFANTGVNFVINSGSGSPYTKYDKPT
                     NGSIVGSLNGSRKPWRTSISMRLDRDIRVKLASAKEDGRKDKYGYLNVYLDISNLLNA
                     KNVLSVYSYTGNPDDDGYLNFSENQSQLATQNDEEAYRNYYSMLINSPYNYTLPRTIR
                     LGVQFSF"
     gene            complement(4851..5213)
                     /locus_tag="PLK75_08010"
     CDS             complement(4851..5213)
                     /locus_tag="PLK75_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39229.1"
                     /translation="MQFRQILFLVLFLLSGIMLSAQSQRYSLDNKETQHKRMVKSRET
                     RKRERLTREEKMVIRIENKQAKAAQYKKKKDARMHAKAVKKHNRKINGQGRNMITGER
                     VHKQMKRSRREAQKNNRR"
     gene            5246..5677
                     /locus_tag="PLK75_08015"
     CDS             5246..5677
                     /locus_tag="PLK75_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39230.1"
                     /translation="MKRFSKILILLLSFVGIFSLSCNNDDRIPYVQVNLQLPLNLAEF
                     QDLKIPGNWVYITGGVRGIIVFCANTDEYYAFERNCTYQPYNDSARIYVDDTNIFAVC
                     EHCGSKFNLYDGYPVSGEAGLPLTQYATSLENDILYIYNRY"
     gene            complement(5794..7209)
                     /gene="ahcY"
                     /locus_tag="PLK75_08020"
     CDS             complement(5794..7209)
                     /gene="ahcY"
                     /locus_tag="PLK75_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOY39231.1"
                     /translation="MTTIISELPYKVKDISLADFGRKEIDIAEKEMPGLMSIRRKYAV
                     DKPLRGARITGSLHMTIQTAVLIETLVELGADVRWASCNIFSTQDHAAAAIALRGIPV
                     FAWKGESLEEYWWCTLQALSFPGGKGPTLIVDDGGDATLLVHKGFYAEADNTLFDKTP
                     ENNEEKIILTTLKMVYAEDKNKWHRMVPAIKGVSEETTTGVHRLYQMFENNSLLFPAI
                     NVNDSVTKSKFDNLYGCRESLSDGIKRATDVMVAGKVVVVLGYGDVGKGSAKSLRGFG
                     ARVIVTEIDPICALQAAMEGYQVALIDDVVSVGDIFVTTTGNKDVITAEHMSKMKDQA
                     IVCNIGHFDNEIQVEQLINWKGIRHLNIKPQVDKYIFPDGHSIILLAEGRLVNLGCAT
                     GHPSFVMSNSFSNQTLAQIDLNTKTYKTGVYRLPKKLDEEVARLHLEQLGVKLTKLTK
                     EQADYIGVPMEGPYKPEHYRY"
     gene            complement(7277..8206)
                     /gene="ribF"
                     /locus_tag="PLK75_08025"
     CDS             complement(7277..8206)
                     /gene="ribF"
                     /locus_tag="PLK75_08025"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOY39232.1"
                     /translation="MKVFQGADIPVLNAPVVTIGFFDGVHLGHCSIIAQLIETARLKG
                     TDHLLITMWPHPTTLVRKKNDDSFLLSTLDEKLLLIEKLGVENVLVLSFDKETADYNA
                     DEFIAKILVEKLKVTTLLMGFNNTFGKGGKPVTAIAKIAEAYNIEFIVAHEYQSTVPI
                     SSSTIREKLSKGNIPEANNILGYQYSFEGIVVSGYKIGRTLGFKTANVKPCFDEKILP
                     GNGVYAVEIIVDSTNYYGVMNIGYRPTFDGKERSIEVHIFDFESDIYGDIITIRLHKK
                     LRNEIRFSNADALIAQLNRDKRIAAKYFALRRQ"
     gene            8319..9491
                     /locus_tag="PLK75_08030"
     CDS             8319..9491
                     /locus_tag="PLK75_08030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY39233.1"
                     /translation="MKNYVIVVILLVFSIVSCKNNSEKNDPNSFTIEGTIANSSGKTA
                     YLLLLHSDKVETVDSVKIGDDNRFSITYSNEQVGFYILKIDKENQIYLIVSAGDTISV
                     EGIYGKLASTYSVNGSDDSENARQLNLHMLESASKLDEFNRIYQAAVQNKEVNIDAVL
                     KELDAEALKLYENDKLFLTNFIHKHEKSPVIYLALFQQLGTTRILNAESDMAVYEFTL
                     EKMKQYHENLEQTKQLEKQINDYKLKQQSSSQNASSEGEAPDIELPTPDGEIIRLSSL
                     RGQYVLLDFWASWCKPCRAESPYLVEAYKKYHEKGFEIYQVSLDSEKSQWVNAIKSDQ
                     LNWIHVSDLKYWDCAPAKVYGVNGIPANFLLDKNGVIIATNLRGKALEAKLSELLK"
     gene            9505..12459
                     /locus_tag="PLK75_08035"
     CDS             9505..12459
                     /locus_tag="PLK75_08035"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOY39234.1"
                     /translation="MRKYYVLINILLVFINISNAQDYFQQEVNYKINVELDDKNHCLR
                     GNIDIEYINNSPDTLSFIYFHLWPNAYKNNKTDFAKQQLLLRSKDFYNTDAVHRGYID
                     SLIFTSGTTGLHLQKTEKEDIALLILDKALQPGDTIMLNTPFFVKIPDSFSRFGHIGQ
                     SYQITQWYPKPAVYDKYGWHYMPYLDMGEFYSEFGRYEVALTLPEDYVVAATGNLVTK
                     QELEWLEDMSLYNSGKRSASVKQRNTQKTKTIRYVENNIHDFAWFANRSFIVTSDTLL
                     LHNSGKVVRCWSFATPENKSQWLQSVEYVKEAVDYYSECIGDYPYNNCTAVDGALSAG
                     GGMEYPGITVISSGGDAYSLERVIFHEVGHNWFYGMLASNERQFPWIDEGFNSFYEYR
                     FFDQKYPEKNLAESFLGESINISGLNHIPHSFELTILYQYLESFGLSQAANTESEAFS
                     PMNYWVMSYQVPVAVLKHLETYLGPEKFDDMMQEFFNNWMFKHPYPEDIRESFEKTTG
                     KNLGWFFDELISSNQRFDYAIKTVEGDSVVIVNRSKVTAPVILCTDSSFLVVEGFTGT
                     KNIAIHGNSLLLSIDPNCITTDFNKNNNYYNRNALFPKWEKTKIMMTGFFDIPGFNEL
                     NILPALGYNTSQKLLLGFIAHNSTIRLKPMEWMVMPLFSPFRTDNVNTAIAGSGKISF
                     NIAGEDNFFRLISLTQTINKYSFDQHISDYDTTYNWISLRTSATIWLKKNHTKPYLDS
                     YLEVSFNTAGILNEKLNRFINLNYTHSDYRIVNPYVFSFHAEQGPGYIKAWSQFCYTI
                     SYPKKNRGLSMRLFGGKFLYNSTEYFGNFNFRLSGDLGLQDYQFQDVYTGRGNDLRDN
                     SSDFWAHQFHRNGGGFVSYTPLGQTNDWLIAVNISANLPIPVLKIFVNAATWSGIREF
                     EYSGYMSWETGLELEVIKSIFSIYLPVLVSKDIQKTNTIYFDNYFERIRFTLNLNLLN
                     PYNYRYKISYLI"
     gene            12456..13202
                     /locus_tag="PLK75_08040"
     CDS             12456..13202
                     /locus_tag="PLK75_08040"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOY39235.1"
                     /translation="MIEKTKIYFVSDAHLGAPGLKSSFEREKLLINWLTEVSADAKEI
                     YLLGDIFDFWFEYKRLAPRGYTRLLCKLADITEAGIPVYFFTGNHDMWVFDYLPLETG
                     VTVCREPVEREYNGKKFYIAHGDGLGPFDKKYKLLKACFKSKFCQWLFQRFHPNFSFW
                     IAHSWSSNSRKKHYLPDKPDYEEEWLVRYSRMVLQKKHIDHFVFGHRHIPYQYKLNEN
                     SMITNLGDWLINFTYAVFDGENMKQMHFLA"
     gene            13601..>16304
                     /locus_tag="PLK75_08045"
     CDS             13601..>16304
                     /locus_tag="PLK75_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39236.1"
                     /translation="MFLLIINNLALAGGINNKGKLWDDNNEPSRFSHILENCISKKNA
                     VADNSISLLNSFVVNNYTYKFEFQKNLNKNSGFEPIIDAVLKNTTNPPITADKTQENH
                     SYSTPENNNNNSFSDSEPGLSEYLAGTSMTISVSSSTICYGSSVNVTWTWNLGDSPQG
                     YAQYSLDNVTWYNMNAGPLATISSGSPKTITPNATTTYYGRVMGNMGVIQLNATPVTV
                     TLTCCSNLDHGGANWTISSNTVLGGSHINVGTFTVNSGVVVTVSPSCNYLYVDAQNIV
                     INGTINANGAGGSGGTGGSYGGLWAQGGYTDGRGITSCWDKDNCRSLGLGGGYRGATG
                     IGTGGGGISYNGSNGTMGYGSKQQCFFIGDEGGMVGGGGGAGAGAGGSYGGLAGSGSS
                     GGQGGSDDDWCGNAGCQSYSEGVGGNGANAYVTYGNTTSQAIEYGSGGAGGGGGGRGS
                     FCYDYDPLTSHTCFTAGGTGGTGGGAVRLIASNNLTVSASGVITANGANGNKGGEGGE
                     NDYTEGCCEDLSPDCTEQTYTAPGGGGGGAGGGSGGGIMLKANCNLTMVGTLQANGGQ
                     GGQGGDGGWSDWSAAYDGGKGSGGGGGGGGRIKIFTNLCSAPNIGGSVSVTGGTGGAV
                     ANYGRSGTGSSGNSGNAGTYNTNTFAIDFTMPANGSSTVPCIANATAPTPPTVYDYCG
                     NIITPTGPSIGGTYNGCEGTRTYTYTYRNCVGGYSHNWVYTYTIERNDFSLPANGSST
                     VACIANATAPTPPTVNDDCGNAITPSGPVLGGTYVNCEGTRTYTYTYADCEGNSHNWV
                     YTYTIEVLPFPNPPDGSSTVDCIADAVAPTLPVVTDNCGNTLTPTGPTIGGTYVNCEG
                     TRTYTYVYTDCEGNTQDWLYTYTIERQDFSLPANGSSTVACIANA"
BASE COUNT         4826 a         3307 c         3319 g         4852 t
ORIGIN      
        1 catcgcaacc cgtatagtca tcggttgcac cacccaagtc agcatctgtc cagacaccaa
       61 aataggtttt gtaaagtgta tatgtagaac ggttgataat gttataattg taaaaagtca
      121 tgtcattgag ctcatcatta gttgtaaaag cgaatgcctg tgccctgagt tccataccaa
      181 tcgggtcgcc tcctgtttca gtgtgaatat ttcctctgtc gttgtaaacc caccataatg
      241 tatagtcgcc aaataatttc gacattctgt ctctgggtct catacagtcg gggtcatcag
      301 taatcccttc attggcaaat tcataatatg ggaagtctcc ctgcaatggg ttataagttc
      361 cgtcaccatc cacatcccag aaaggagcga gataataatc gtaaccacct gaaggaccat
      421 gtgcaggcca attcaaaatg atatccggaa tagtgtatgt actgaagttt tctgccacat
      481 cacagtcagg gtcttgtgaa caagcatacc actcacggaa ttccttaacc atctcctttg
      541 agattacaaa atgtttgtcg tattccctgc agatttcctg agaagtagtt ccttgcaaat
      601 taccagaaac aatcagagga ccaggccaaa aatcgatacc tacctggcgg aaacgttggg
      661 ctgccaattt aagctgacca ttggcgtctt tacctccaat ccagatagaa cctgcaaaaa
      721 gtgcatttct gcctgaacct gcaggaactt catatcgggg gtctccctgg aggtcccacc
      781 acatatcgcc gccggtatgg atcaaagccc taacattatt gatatataac tctgtagaag
      841 tttttgaggg ttcgcaagcc gctgcagccc gtgtgttttt ttgattgtcg ttgttctggc
      901 tgggaattct gggatcctta tcagccatgg cattaattgt taaacacact gcaatcgaag
      961 cgaatacaaa ttttcttaat cttctcattt tatgctgtct tttagaattt cacaatattt
     1021 ctttaaaaac tgaactgaac accaagcctg attgtccgtg gcaaggtata attataagga
     1081 ctgttaatga gcattgaata ataattccga tatgcttcct cgtcgttttg tgttgccaat
     1141 tgactttggt tttcggagaa attcagataa ccgtcatcat cggggttccc agtataactg
     1201 tatacgctca gaacattctt tgcattcagg aggttggaaa tatcaagata tacattcagg
     1261 taaccatatt tatcttttct tccgtcttct ttagcactgg caagttttac ccggatatct
     1321 ctgtcgagac gcatgctaat gcttgttctc caaggcttgc gagagccatt cagagaacca
     1381 acgattgaac cattggtagg tttatcgtat tttgtatagg gcgatccaga tccactgttt
     1441 ataacgaagt ttacaccagt attggcaaaa atatcagcac cgaaccatcg tggaccatca
     1501 taaggtgttc ctgacacttt tccgccaaaa cgatagtcaa cggtaatggc aatattgtgt
     1561 ctttggtcga agtcgagtgg aatagaagta cgcaaattcg gctgatctga tttgataata
     1621 gcagcagcag attccacatt tgaaccagtt ccattggcaa actgcaatgt ataagccacc
     1681 ctcatggtaa catttccggt acggcgcaaa tcgtacgatg cagtaaagcc tttcacagtt
     1741 ccaaaatcga tattaccata agtgtaataa ttaaccgggt atgcaccata cactgcaact
     1801 gcctgctgca tatcgcgcat ttcgcggtag aatgctgaca atttcagaga agaagtgtta
     1861 ccgagttttt gctggaatcc caactcatag tcaatggttt tttcagtttt cagattggga
     1921 ttattcagaa tatctccgtt tttctgacga ataaaatagt attcaatcag atcgagtctg
     1981 ttgtagtagg ttggtctttt tgaaagaatg tcatagtgag caaaaaacag tgcctcatcc
     2041 gaaatgggga atgagaaaga aatacggggc ataaacacaa gctgaggctt atagtcttcg
     2101 aaagctccag cactcaattc tgtttctttg tcaattagat agggtgcaat tccattagca
     2161 ctggcaattc tgtttgggtt agaaacctcg gtaccagctg catcatacca tacattgcca
     2221 acacgatagc cattaattcc ggatgggtta tttaaatcgt ttacataaac aacagcattg
     2281 tcggggatat tgcttgggtg ttcaaccccg gcgataagtc cctgcgggtc gttatcgccc
     2341 acggtgtagg cttcactgaa gaggaactga tctttcagta ctttttgatt ggcatcatac
     2401 cggtcaacac gtaacccaat attaaatata aggtctttaa aagcaaactt atcctgaata
     2461 tatccggcaa tataattagg ctggaatgaa ggaattggac gggtgagaac gccatttgca
     2521 tcggtttggg taaagaagtc ttcgatagtg gcgttgttgt ttaatttttt tcctgtgtgg
     2581 tcgtagccat agtatgccac atagttatta ccctgattga atagttcgtc agcactgaag
     2641 taggaaatat caaactgatc aggatcgtat gaatcgatat caatccaatt taaacttgta
     2701 atgtcataac ccaaataagt tctaaagttc ttatcgaatg ttgattgtgt ttcggcgttg
     2761 tataacctcg gataattaat tgtatcaagg taaactccat tggcatctct ttcaatgatt
     2821 gggttagcaa ggtctttttc gttgatgtga tagttcatca gtttacgtgc gagtgaccaa
     2881 agcccaaatg gagccagtcc ataataagaa tcatttcgtt gttcgaattc aaaaccgaag
     2941 ctgatctcat ggtctttaat gtcggcagta ccactggctg atacgcggaa ctgtgtagcg
     3001 tcagagatgg tataactggt atatggattt ccggggctgg cccaaagtcc gtaaaccgaa
     3061 ggcgcactct gcccgttcaa caaacctccg ttgagttcaa cgtcttcttt acgcaagaga
     3121 gagtaatcgc tgaaataatc gaagaactgt gttgaataaa ttgcaagatc ttcgttatag
     3181 gttgaaggaa taaaagtatc gattgcataa acaaaaactt cctgcagcca cccttcgagg
     3241 tttgctactg tatcgacacc atagttgtaa aatttcaaaa catcggtctg gaaagttccc
     3301 acatgaccgt attggaagaa attgtcgcga taggtacgat cgaaatagtt tgaataggtt
     3361 tttgaatagt caacctgaat tgtatagtag gcattacgaa taacagagtt atcttcttcc
     3421 tcatctgatt ttccgctatc aaatttctgg gttaatcttc cgtaaaccct ccagttatta
     3481 taagcgtaag ctccattgtt ggatgaattg aataaatcgt tgcttcttga atatgcacgt
     3541 ccgagtctat aatccatagt acctccaaaa gtgaggttca ggttgcgaac cggttggaag
     3601 tctatttttc cggcaagatt aacctgcaaa ttttcggcat ccgggcgtgt tttctcgttt
     3661 gtgaatgcat cggcacccag gtagtcggca ttctgataaa ttatagcacc gttttcggtc
     3721 tgaacaagcc tcagcggatt agttctgatg ctttccacta cttcgggttt agcgcgccat
     3781 actccaacgg cagacggata atcatcgata atattattta cttcacccga aatcaggaag
     3841 cctgcaactg tacgttttcg gtttgttcct ttttcttcga ttgtccatat agggcccgac
     3901 agcataacgc ctccgagatt gtatccgtaa ggatcgagga actgggaagt aaggaattct
     3961 gcaccgccat aatatttact ggtcgggcct tttgtggtaa ccgaaataat accaccggta
     4021 gcatctccat atttagcagc aagccccccg gtgataacgg ttacctgttc tattgcagat
     4081 ttgggtatag aactcgatcc ccgcacttta acgccatcaa catagtaaac agtaccttcg
     4141 gagcgggcac cacggatact acccacttca ccgtcttcgg aataaacccc tgctactgtg
     4201 gtagcaactg cggtggcaga acgccccggc atcttggcaa tatcttcgga agtaactgtt
     4261 cctcccgatt cagtttggtc ttttgcaatc agtggaacct tatagtcctt gatttcgact
     4321 tcctgcagaa tttcagccga agctttcaga tcaaaatcct gaaaggtaat cttacctgcc
     4381 ggtatctgca cgttattata ctgcaatgtt gcataaccaa catatgaggc tttgaccgtg
     4441 tatttaccgg ccggaattgg tttaatcgaa aatttaccat cgaaatcagt cattccccca
     4501 gtaacaattc gtccgttttc ttcgacggaa acattggcaa aaggaattgg ttcgcctgta
     4561 gcagcatctt ttaccgttcc gctaaaagtg cctgattgtg caaaaatact tgcgtaaaat
     4621 gccatggcaa cagtcaatag aagtagtctt tgtaacataa tcctgtattt actttttaaa
     4681 atacttaacc cattttaaac aaactgtaaa tataaaaccg attaataaac cagccaaact
     4741 tttttttcca aaatcaaaga ttatttaaaa aattgttaaa aaaatatagg atgaattatt
     4801 cttattatct gataatcatt atattattat agtaaaatta tataacatac ttatcttctg
     4861 ttattttttt gcgcttctcg cctcgatctt ttcatttgct tatgcaccct ttcaccagta
     4921 atcatatttc taccttgtcc gttaattttt cggttatgct tttttacagc tttggcatgc
     4981 attctggcat ctttcttttt cttatattgt gctgcttttg cctgtttgtt ttctattcgg
     5041 ataaccattt tttcttctct cgtaagtcgt tcccgtttac gggtttcacg cgacttaacc
     5101 attcgtttat gctgggtttc tttattatca agagaatagc gctgcgactg ggcagaaagc
     5161 attatacccg acagtaaaaa taaaaccaaa aaaagaattt gcctgaattg cataaaaagc
     5221 gtatttttga atttatattt tacaaatgaa acgtttttca aagatattaa ttttattgtt
     5281 gtcgtttgtc ggaatttttt ctttgtcatg taacaatgat gacaggattc cttatgttca
     5341 agtaaatttg caacttcctt taaaccttgc tgagtttcag gatctgaaaa tcccggggaa
     5401 ttgggtttat ataacaggtg gtgtccgagg aattattgta ttttgtgcaa atactgatga
     5461 atactatgcc ttcgaaagga attgcacata tcagccttat aacgacagcg ccaggattta
     5521 tgttgatgat acgaatattt ttgctgtatg tgagcattgc gggtccaaat tcaatcttta
     5581 cgacggatat cccgtatcgg gagaagccgg gttgccattg acccaatatg caacctcact
     5641 cgagaacgac atattatata tatataatag gtattagcat aagcaaggtc ttaaaaattt
     5701 cactcttaga agcaatcaca ggtataatta tctattgcaa aaaaaagagg ccttgcagcc
     5761 tcaattattt tctttctcat ttcatataaa tctttaatat ctgtaatgct cgggtttata
     5821 aggaccttcc atcgggactc ctatataatc ggcctgttcc ttggtcagct ttgtaagttt
     5881 aacgccaagc tgttctaaat gaaggcgtgc gacttcttca tcaagttttt tgggaaggcg
     5941 gtaaacgccg gttttataag tttttgtatt tagatcgatt tgtgccaagg tctgattcga
     6001 aaatgaatta ctcatcacaa acgacggatg acctgtagcg caacccagat tcaccagccg
     6061 cccttcagca agtaaaataa tggaatgacc atcggggaat atatatttat cgacttgtgg
     6121 ttttatattc agatgcctga ttcctttcca attgataagc tgctcaacct gaatctcatt
     6181 atcgaaatgc ccgatattgc agacaatggc ctggtctttc attttcgaca tgtgctcggc
     6241 ggtaatcaca tctttgtttc ctgttgtagt aacaaaaata tcgccgactg aaaccacgtc
     6301 atcaatcagt gcaacctgat agccttccat agcagcctgc aatgcacaaa ttgggtcgat
     6361 ttcagtgacg attacccttg caccgaagcc tctgagcgat tttgccgaac ctttgccaac
     6421 atctccatag cccagcacca caaccacttt gccggcaacc ataacatcgg ttgcgcgttt
     6481 aataccatcc gacaacgatt cgcggcatcc atagaggttg tcaaatttcg attttgttac
     6541 cgaatcgtta acattaattg ccggaaataa taggctgttg ttttcgaaca tctgatacag
     6601 tcggtgaaca ccggtggttg tttcttctga aacacccttt attgcaggaa ccatccggtg
     6661 ccatttgttt ttatcttcgg cataaaccat cttaagggta gtgagaataa tcttttcttc
     6721 gttgttttca ggagttttat cgaaaagggt attatctgct tcggcataga atcctttgtg
     6781 caccaacaaa gtagcatctc caccatcatc aacgattaaa gtcggacctt tcccaccggg
     6841 gaatgaaaga gcctgaaggg tgcaccacca atattcctca agactttcgc ctttccatgc
     6901 aaacacagga attcctctta gggcaattgc tgccgctgca tgatcctgag tggagaaaat
     6961 attacaactt gcccagcgga catctgcccc cagctctaca agagtttcaa taagcacagc
     7021 agtttgaata gtcatatgaa gcgaccccgt aatacgtgct cccctgaggg gtttatccac
     7081 agcatatttt ctccggatac tcatcaatcc aggcatttcc ttttcggcga tatcaatctc
     7141 tttgcgcccg aaatcagcaa gcgatatatc tttaacttta tagggcaatt cagaaattat
     7201 tgttgtcata acgtaaatat tttagcgcac aaagatactt tataaatatt aaagaaacaa
     7261 taaaagcatc gcattttcac tgcctccgga gggcaaagta ttttgcagca attcgtttgt
     7321 cgcgattcaa ctgagcaatc aaagcatcgg cattgctgaa gcgaatttca tttcgcagtt
     7381 ttttatgtaa ccggatagtt attatgtcgc cataaatatc ggattcaaaa tcgaatatgt
     7441 gcacttcgat gctgcgctct tttccatcga aagtcggcct gtaaccaata ttcataacac
     7501 cataataatt cgttgaatca acaattattt caaccgcata aactccgttt cccgggagaa
     7561 ttttttcatc gaagcaaggt tttacgttag cagtcttaaa gccaagcgtt ctgccaattt
     7621 tgtaaccgct tactactata ccttcaaatg aatattgata acccaatatg ttattcgctt
     7681 cagggatatt tccttttgag agcttttcgc ggattgttga ggaacttatt ggcacagtgc
     7741 tttgatattc atgagcaacg ataaattcaa tgttgtaggc ctccgcaatt ttggcaattg
     7801 cagttaccgg ttttcctcct ttgccaaaag tattattaaa gcccataagc aaggttgtga
     7861 ctttcaactt ttcaacaagt atttttgcaa taaattcatc tgcattataa tctgcagttt
     7921 ctttatcgaa actcagcacc agtacatttt cgacacccaa cttctctatc aacaacaact
     7981 tttcatcgag tgtggagagt aaaaaactat catcattttt tttccttacc agtgttgtag
     8041 ggtgaggcca catagttata agaagatggt cggttccttt cagacgtgct gtttcaatta
     8101 gttgtgcaat aatactgcaa tgccccaagt gcaccccgtc gaagaaacct attgtaacca
     8161 cgggtgcatt aagtacaggt atatcggctc cctgaaagac cttcattcca aaataatatg
     8221 ccgtaaaaat aataaatgta tcgataaaag aattattaat gaacaaacaa cataataact
     8281 cgttattaag tttaattata tttgtaaaaa ttaaccttat gaaaaattat gtaattgttg
     8341 taattttgtt agtattttca attgtttcat gcaagaataa ttctgagaaa aatgatccca
     8401 attcatttac aattgagggt acaattgcaa attcatcagg aaaaacagcc tatctgcttc
     8461 ttttgcatag cgacaaagtg gaaaccgttg actcagtgaa aattggcgac gacaatcggt
     8521 tcagtattac ttacagtaac gaacaggtcg gtttctatat attgaaaatc gacaaagaga
     8581 atcagatata tttgatagtt tcagccggcg acaccataag tgttgaaggt atctatggaa
     8641 aacttgcttc tacctattcg gtaaacggat ctgatgattc cgaaaatgcc cggcaactga
     8701 atcttcatat gctcgaaagt gcgtcgaagc ttgatgagtt caacagaatt tatcaggcag
     8761 ctgttcaaaa caaagaagta aatattgatg cagtattaaa agagttggat gctgaagccc
     8821 tcaaacttta cgaaaatgat aaattatttt tgacgaattt catacataaa cacgaaaaat
     8881 cgccggtaat atatcttgca ttgtttcagc aattggggac aacccgcatc ttaaatgctg
     8941 aaagcgatat ggctgtttat gaattcacac ttgaaaaaat gaagcaatat cacgaaaacc
     9001 ttgagcaaac aaaacagctg gaaaaacaaa ttaacgacta caaactgaag cagcagtcgt
     9061 catcgcaaaa tgcttcatct gaaggtgagg ctcccgatat cgaacttccc acgcccgatg
     9121 gagaaataat aaggctgagt tcgttgcggg gacaatacgt attgctcgat ttctgggcat
     9181 cgtggtgtaa accatgccgt gctgaatcac cataccttgt ggaggcttat aaaaaatacc
     9241 atgaaaaggg tttcgaaatc tatcaggttt cactcgactc ggaaaaaagc cagtgggtaa
     9301 acgcaataaa atcggaccaa ctgaattgga tacatgtaag cgatttgaaa tactgggatt
     9361 gtgctcctgc aaaagtatat ggcgttaatg gcattcctgc caattttctg ctcgacaaaa
     9421 atggcgttat aatagccaca aatttgagag gtaaagctct agaggcaaag ctttcagaac
     9481 tattgaaata agatagcact ttctatgaga aaatactatg tcctgatcaa tattcttctt
     9541 gtatttatta atatatcgaa cgcccaggac tattttcaac aggaagttaa ttataaaata
     9601 aacgtagaac tcgacgataa gaatcattgc ctgaggggta atatcgatat tgaatacatc
     9661 aacaattctc ccgatacgct gagttttatt tattttcatt tgtggcccaa tgcatataaa
     9721 aacaataaga cagattttgc aaaacagcag cttcttctgc gtagtaaaga cttttataac
     9781 accgacgcag ttcatcgggg atatatcgac agcttgattt ttaccagtgg cacaacagga
     9841 ctgcacttac aaaaaacaga aaaagaggat attgctttgc taatccttga caaggcactt
     9901 cagccgggcg ataccattat gctaaataca cccttttttg ttaaaatacc ggactctttc
     9961 agcaggtttg gacacatagg gcagtcttat caaatcacac aatggtaccc caaaccagca
    10021 gtgtacgaca aatacggttg gcattatatg ccatatcttg atatgggtga attttattct
    10081 gaattcgggc gttacgaagt tgctcttacc ctacccgaag attatgttgt ggctgcaaca
    10141 ggaaaccttg ttaccaaaca ggaacttgaa tggctcgaag atatgtcgct atacaatagc
    10201 ggcaaacgat ctgcatcggt aaaacaaaga aatacgcaga aaacaaaaac aatccgctat
    10261 gtcgaaaaca atattcacga ttttgcatgg tttgccaatc gcagttttat tgttacttcc
    10321 gatacattat tattacataa ttcgggcaaa gttgtcaggt gctggagttt tgcaacaccc
    10381 gaaaataaat cgcaatggtt gcaatcggta gaatatgtaa aggaagccgt ggactattac
    10441 tccgaatgca tcggagatta cccatataat aactgtactg ctgtcgacgg agctctttct
    10501 gcaggcggag gaatggaata tcccggtata accgtgatat cttccggcgg ggatgcatat
    10561 tcgcttgaga gggttatttt tcatgaagtt ggacacaact ggttttacgg aatgctggca
    10621 agtaacgaaa gacaatttcc gtggattgat gaagggttta attcatttta tgaatatcgg
    10681 tttttcgatc agaaatatcc cgaaaaaaat ctggctgaat cttttcttgg agaaagcata
    10741 aatatttcag ggttgaatca cattcctcat tccttcgaac taactattct ataccaatac
    10801 cttgagagtt ttggattatc gcaggcagcc aacaccgaat cggaagcatt cagtccgatg
    10861 aattactggg taatgtcgta tcaggttcct gttgctgtac tcaaacatct tgaaacatac
    10921 cttggcccgg aaaagttcga tgatatgatg caggagtttt ttaataactg gatgttcaag
    10981 cacccttacc ctgaagacat ccgggagtct tttgaaaaaa caacagggaa gaatctgggt
    11041 tggttttttg atgaattgat ttcttctaat cagcgttttg attacgcaat aaagacagtt
    11101 gaaggcgact ctgttgtaat cgtaaataga agcaaggtta ccgcaccggt aattttatgt
    11161 accgacagca gttttttggt agtagaaggg tttacaggca ctaaaaacat tgccatacac
    11221 gggaactcat tattactgag cattgacccc aactgcatta caaccgattt caacaaaaac
    11281 aacaattact acaacagaaa cgcattattt cccaaatggg agaaaacaaa aattatgatg
    11341 accggatttt tcgatatacc gggcttcaac gaattgaaca ttttacctgc attgggatat
    11401 aatacaagtc aaaaactatt actcgggttc atcgcgcata acagtactat ccgtttaaaa
    11461 ccaatggaat ggatggtaat gccacttttt tcacctttca gaaccgataa tgtgaataca
    11521 gcaatagccg gcagcggtaa aatatcattc aacatagcag gagaagacaa tttttttagg
    11581 ttaatcagcc tgacacaaac tataaataag tatagtttcg accagcatat ttcggattat
    11641 gataccactt acaactggat cagtttaaga acttcagcaa caatctggct gaaaaaaaat
    11701 cacacaaaac cctatcttga ttcgtatctg gaggtttctt tcaatacggc aggtattctg
    11761 aacgaaaaac taaacagatt tataaatctg aattatactc acagcgatta ccggattgta
    11821 aacccctacg ttttttcttt tcatgcggaa caagggcccg ggtatattaa agcatggagt
    11881 cagttttgct atactattag ctacccaaaa aaaaacagag gcttaagtat gcgattattc
    11941 ggtggcaagt ttttatataa ttcaacagaa tatttcggca attttaattt cagactttcc
    12001 ggggatctcg gcttacaaga ttatcagttt caggacgtgt ataccggcag agggaacgac
    12061 ttacgcgata attcgtctga tttctgggct caccaatttc accggaacgg gggtgggttt
    12121 gtttcgtata ccccattggg gcaaaccaac gactggctta ttgctgtaaa tatctctgct
    12181 aatttaccca tacctgtgct gaaaatattt gtaaatgctg ccacctggag tggtattcgc
    12241 gagttcgagt attccggtta catgtcatgg gaaacggggt tagaacttga agtaataaaa
    12301 agcattttca gcatttacct acctgttctt gtttcgaagg atattcagaa aacaaacacc
    12361 atttatttcg ataattattt tgaaagaata cgatttactt taaatttgaa ccttttaaat
    12421 ccgtacaatt atcgttataa aatttcttat ttgatttgat agaaaaaact aaaatatatt
    12481 ttgtttctga tgcccattta ggggcaccgg gcttaaaaag cagtttcgaa agagaaaagc
    12541 ttctgataaa ttggctaaca gaagtatcgg ctgatgccaa ggaaatatat ttattgggag
    12601 atattttcga tttttggttc gaatataaaa ggcttgctcc ccgaggttat acccgtttac
    12661 tatgtaaact cgctgacata actgaagcag gaattcccgt atattttttt accggaaacc
    12721 acgatatgtg ggtattcgat tacctgcccc tggaaaccgg cgttaccgtt tgcagagaac
    12781 ccgttgaaag agaatacaac ggaaagaaat tttacatcgc ccatggagat gggttgggtc
    12841 ctttcgataa aaaatacaaa ttgttgaaag catgcttcaa atcgaaattt tgccagtggc
    12901 tattccaacg ttttcatccc aatttctcat tttggattgc acactcatgg tcgagcaaca
    12961 gccgaaaaaa acactatctt cccgataaac ctgattacga agaggaatgg ttggtgcgat
    13021 attccagaat ggtattacag aaaaaacaca tcgatcactt tgtatttgga catcgtcata
    13081 tcccgtacca atataaattg aacgaaaaca gtatgattac taatttgggc gactggctaa
    13141 ttaactttac ctacgcggtt ttcgacggag aaaacatgaa gcagatgcat ttcttggctt
    13201 agcaactatg tgtttctgat ttcttgcaaa ctcgcgtcta attagatttc attccatagg
    13261 ttttaccaag acccctgaat tcaatactgc tacaagtaaa tacataacaa gttttgtcga
    13321 tttggtgaca accaaaatat aattacttcg tttaaactta aaatagtttt tttatatacg
    13381 ggtagttttc ttttatgata ttaaaaacag aattaataaa atctagctat tgtaaatttt
    13441 tatctgattc aactattaat attttataaa ttttactatc tttcgtttct tatatataga
    13501 ggaaatgtat aaacatatct caaccatgaa aaagccaaac caagcttatt tgctgaaact
    13561 ttctttattg ataaaatcag tggcgatatt ctccatttat atgttcttac taattattaa
    13621 taacctggca ctggcaggtg gcattaacaa taaaggaaaa ttatgggatg ataacaatga
    13681 gccatcaagg ttttctcaca ttttagagaa ttgcatctca aagaaaaacg cggttgcaga
    13741 taattccatt tcattattaa acagttttgt cgttaataac tatacctata aatttgaatt
    13801 ccaaaaaaac ttaaataaga actctggctt tgagcccata attgacgctg ttttaaaaaa
    13861 cacaacaaac cctccaataa ctgcggataa aacacaagaa aatcatagct actctactcc
    13921 agaaaataac aataataact ctttttccga ttcagaaccc ggattatcag aatatttggc
    13981 tgggacatcg atgacaatta gtgtaagttc ttccactatt tgttatggtt cttctgtaaa
    14041 tgttacatgg acatggaatc tgggagactc tccgcagggt tacgcgcagt attcgcttga
    14101 caatgtgact tggtataata tgaatgcggg gccattggcc accatttcat caggctctcc
    14161 aaaaacaatt acaccaaatg caactaccac gtattatgga agggttatgg gtaatatggg
    14221 agtgatacaa cttaatgcaa ctccggtaac cgttactctt acttgttgca gtaatcttga
    14281 ccatgggggt gcgaactgga caatttcatc gaatacggtt ttaggaggca gccatattaa
    14341 tgttgggaca tttacagtga attccggagt tgttgttacg gtttctccca gttgtaatta
    14401 tttatatgtt gatgctcaaa atattgttat aaatggcaca atcaatgcca atggcgcagg
    14461 gggctcaggg ggaaccggag gaagctatgg cggcttatgg gctcaaggag gctacaccga
    14521 tggccgcgga attactagct gctgggataa agacaattgt aggtcgttag gccttggagg
    14581 agggtaccgt ggagctacag gaattggcac tggaggaggg ggtatctctt acaatgggtc
    14641 aaatggtaca atgggatacg gttcaaagca gcaatgcttt tttatcggag atgaaggagg
    14701 tatggttggt ggtggtggcg gtgccggcgc cggcgccggc ggttcttacg gagggctggc
    14761 aggtagtggt tcttccggag ggcagggtgg atccgacgac gattggtgtg gtaatgcagg
    14821 atgtcaatcc tacagtgaag gagttggcgg gaatggggcc aatgcatatg taacttatgg
    14881 taatactact tctcaagcaa ttgaatatgg atcgggcggt gctggtggtg gtggtggcgg
    14941 tagaggctct ttctgttatg attacgaccc tttaacttca catacctgct tcactgcagg
    15001 agggactggc ggtaccggag gtggtgctgt ccggttaatt gccagtaaca atctaacggt
    15061 ctcagcatcg ggtgtaatta ctgctaatgg tgccaatgga aataaaggtg gcgaaggagg
    15121 agagaatgac tatactgaag ggtgttgcga agacttaagt cctgattgca ctgaacaaac
    15181 atatactgca cccggtggtg gcggtggcgg tgcaggcggc ggttccggtg gtggcattat
    15241 gttaaaagca aattgcaact taactatggt gggtaccctg caagccaatg gcggtcaagg
    15301 tggtcaaggc ggcgatggag gttggagcga ctggtcggct gcatatgatg gtggcaaagg
    15361 aagtggtgga ggtggaggtg gaggtggccg aattaaaata ttcacaaacc tctgttctgc
    15421 tcccaatatt ggagggtcag tctctgttac cggtggcacc gggggggctg ttgcaaatta
    15481 tggaagaagt ggaaccggat cctctggaaa cagcggtaat gccggtacgt ataatacaaa
    15541 tacatttgca attgacttta caatgccggc caacggaagc agcactgtac catgtattgc
    15601 caatgcaact gctcctacgc cgcctacagt ttacgactat tgcggaaata taataacccc
    15661 taccggtcct tccattggag gtacatataa tggttgcgaa ggaaccagaa cctacactta
    15721 cacctatcgg aattgtgtgg gcggatattc ccataactgg gtatatacct atactatcga
    15781 gcgcaacgac ttctcgctac ccgctaacgg tagcagcacc gtggcttgta ttgccaatgc
    15841 aactgctcct actcccccaa ccgtgaacga tgattgcggt aacgcaatta ctccatccgg
    15901 accagttctt ggtggcactt atgtcaattg cgaagggact cggacttata cctataccta
    15961 tgccgactgc gaagggaact ctcataactg ggtatatacc tacactattg aagtactccc
    16021 gttccccaat cctcccgatg gaagctctac cgtcgactgt attgccgatg ctgtggctcc
    16081 tactttgccc gtggtaaccg acaattgtgg caataccctg actccaacag gacccactat
    16141 cggaggtaca tacgttaact gcgaagggac caggacttat acttatgtct acaccgattg
    16201 cgaaggcaat acccaggact ggctgtatac ttacactatt gaaagacagg acttctcgct
    16261 acccgctaac ggtagcagca ccgtggcttg tattgccaat gcaa
//
LOCUS       DAOOQP010000068        16277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4344_length_16277_cov_5.485637, whole genome shotgun sequence.
ACCESSION   DAOOQP010000068 DAOOQP010000000
VERSION     DAOOQP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_16277_cov_5.485637"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="PLK75_08050"
     CDS             <1..1940
                     /locus_tag="PLK75_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOY39237.1"
                     /translation="YFSNTVLLQNLEKQNRTKTKLKQYLILLTRILLIVALVTAFSQP
                     YIPVNKNQNQGGKKYTAIYLDNSFSMDGESEKGKMIDAARQLAYDIVSAYPPDMQYFF
                     LTNHFSPEYLQLMNTNEIKENIADVNLTPMFRSLPEVLARFSALAGQDATLNIYVISD
                     MQECNFKSDITFSSFSHHVVLVPITGNLQGNLSVDSCWFNAPVKNIGKADELTVQIKN
                     HSNQPYVDIPVKLSINDTVKTISSLTIEAGETVKLPLTFTNTSSGNIKGEVGIDDYPV
                     TFDNNLFFSYSINNVVNILIINETGTNQYLSALYKTADDNFNYTEVKAGSEKNSEFQQ
                     YQLIIANGLKSIPSGLAESFKEYIETGHSLVLIPGEQCDLGSYNSFLSALEVGKISEV
                     SLQQRRISEINYRNYLFKDVFGKIDKNIDLPDINSFYPLQVFSNSNADVVLFDDKSNP
                     LIATNPTGAGKFVFCAIPLSKTNELFMKHPLFVPLFYNMALYSGSSQSLYCVAGRESY
                     IDISGFRPDQNTVLHIVDSLTDIIPASRIIPGGTRITIPETLVRAGHYSVTSGNATHS
                     LISMNYSREESATKYLQGNEIIEELKQAGIESVSSVDYLSENFRSNVAEQSLGTMLWK
                     YFIILALVFVVAEIAIIRLMK"
     gene            complement(1949..3103)
                     /locus_tag="PLK75_08055"
     CDS             complement(1949..3103)
                     /locus_tag="PLK75_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39238.1"
                     /translation="MNYSFNRNHAVIALQHLKMIAPGAVCGYCIYLSQEDNLLHYTRF
                     FRGNEPEDNVISDEDNIISNLRKPRKTPIQWFTPDNVPIKNNISGITQLEIFSESEHT
                     VMCLRIDSMADSNQDLVFIELKQDLSAFGINPADGKLEVQTKKILGLLLHNSVMAVIR
                     AAESDARLHRDLSRNTLMLIDTVKKSKEAVCMEQSMHKQSIERLAKEYLDGISQQYNR
                     KILLSDDAAEKLWKQREAIHKINHILANAVQFAVNITATDPSTPLIIGEELLFLNESS
                     DRNNQPPHVTSVTRTISKELKAEQFLYKLLRSVEKLLAKGHNITGKMVGANCEPSVSA
                     PAITEFIKKYRKQILNLIKEDPSKWALLINEFRPIQNVYYSSPGVISKTG"
     gene            3450..4523
                     /locus_tag="PLK75_08060"
     CDS             3450..4523
                     /locus_tag="PLK75_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOY39239.1"
                     /translation="MFNKNGLSLKKLNGIKAFVQKLLEYYREECNRRGLVVFVDNTFL
                     VQKEQWIYDKASCKSHAIKKQTEKNLPGFVKVHHSETYSKSGNVSSIENQQRGDLMYS
                     FHWNNEFESLVLQAIATDVSNVEFYPKSSLQQHKSSNKSVMVLNFRLNKVLLEKYYCD
                     ELIYSDTIGTGHNTIIENIARTFGISQQHAAKMYVMYGSVFLDKSLTDLFIDVKLNEF
                     HVNEVPYAQLCSLIQEGYIDMLKKVSARLSSKKVSPECDRIMVSGKNISQKVPQLVSL
                     FFNTECNQVVDAFDFSYNELVSFVPATKELPIMTAAEVEICENIIPEPRKSSFQKKSL
                     RVINAIMNEIREFMTEPEAEFIS"
     gene            complement(4513..5199)
                     /locus_tag="PLK75_08065"
     CDS             complement(4513..5199)
                     /locus_tag="PLK75_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY39240.1"
                     /translation="MKKDKILLVEDDINFGAVLKSYLEINDYEVMLVNDGMRAVPVFR
                     STSFDLCILDVMLPNKDGFSIAMDIRKIDEGIPLIFLTAKSLKEDVLRGFEVGADDYL
                     TKPFDSEVLLRKLKAILRRNKESFDDIPNMIEIGPYIFDHQLRTLTGNNSTFRLTPRE
                     SDLLKMLYANKNHVLDRNVALKHIWGDDNYFNARSMDVYITKLRKYFKDIDSIDINNV
                     HGSGFILVMK"
     gene            complement(5206..6750)
                     /locus_tag="PLK75_08070"
     CDS             complement(5206..6750)
                     /locus_tag="PLK75_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY39241.1"
                     /translation="MKKKRTVYLLAGLMIVSLLTIIAMQAGHIYRSYNEKKELVDRGV
                     RESLSQALIKLEKRDALVFLYNKMLNSRSAQNDSTYPADPNMSRFGYIPPQPELPIHN
                     FSISIRSSYGGVQVYEFSNTWGNDYYPEQFLSYSTFERFFDEKFNTGGLALDEVVSEL
                     EKEYKQMRRPLENRFDKEYIRKAISEELKQRGLSLNFEFAVADANHNIRLKTDGYNES
                     ITDDAYKINLYQGAIFSAPDALYIYFPGKRKYVLEGIYAQLISTVVLSLILIITFAIT
                     LVAFFRQKKLTEIKNDFINNMTHEFKTPLATIQLAAASINNPRVRENPKTVDKFTEII
                     NQETQRMNQHVEQVLQLALLDRESLNLKKEPVDMHELIKDAISNIELIIQQRNGDVRT
                     DFLADIPVIKADRDTLLNVVGNLLDNANKYSVDQPDILVRTYNSGNHFVFEVEDKGIG
                     MSKDVMKRVFDKFYRASTGNVHNIKGFGLGLNYVKEIVAAHKGNISVKGNHSKGSTFI
                     VSLPIN"
     gene            complement(6848..6982)
                     /locus_tag="PLK75_08075"
     CDS             complement(6848..6982)
                     /locus_tag="PLK75_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39242.1"
                     /translation="MYTKEIISLLMWPVLILAAYLISLAALRHFDKQEDKTNVEAEEK
                     "
     gene            complement(6994..8127)
                     /gene="cydB"
                     /locus_tag="PLK75_08080"
     CDS             complement(6994..8127)
                     /gene="cydB"
                     /locus_tag="PLK75_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOY39243.1"
                     /translation="MASITFAALQHYWWFLVSVLGSALVFLLFVQGGQMLIYTLGKNE
                     TERSMLVNSLGRKWEFTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWLIILLAFTVQA
                     VSYEFRSKPSNVFGKKTFETLLFINGFLGTILLGIVVATLYNGAEFSLNTMNHVKWDN
                     PARGLEAALNYHNLLLGFSVFFLSRVLALLYFRKTIENNEICNRIKKQLIINAVPFVL
                     LFVSFVTILLLKTGYAIDPETKSIVFEKYKYLHNLIQMPLVAIMFIAGVLAVLWGIIA
                     TVVSDKYDNGIWFTGLGTFFAVLALLLIAGLNNTSFYPSITHPESSLTVYNASSSEYT
                     LKTMAIVSLIIPFVFAYIVVAWRSINKRKITRSEIEDSNEHHY"
     gene            complement(8130..9680)
                     /locus_tag="PLK75_08085"
     CDS             complement(8130..9680)
                     /locus_tag="PLK75_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOY39244.1"
                     /translation="MLLNVDPSLVDWSRAQFALTAMYHWIFVPVTLGILFIIAFMETI
                     YVKTGDPEWKRTTKFWMKLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGIMAFFMESTFIAVMFFGWNKVSKKFHLASTWLTAIGANLSALWILVANGWMQFPT
                     GMYFNPDTARNEMVNFWDVFLSPVAVNKFLHTITSGYIVASLFVIGISSYFLLRGRES
                     LLAKRSIIIASVFGLISSIAIILTGHGSAVQVAEKQPMKLAAMEGLYKGGTNAELMGF
                     GIITPGKQAFDGKNDYVVKFGFKGMLSYLIAFNKDHYVPGVEDLINGVPEKGILPAQE
                     RIDKGKIAVSSLKAYKIAKKNGNTAEARKMLALFNENYKHFGYGFLDKPTDIIPNVQL
                     VFYSFHAMVLLGTFFVFVFLAFFWFSFRNTIEKFRFRKLLYYIALLCIPLSFIASEAG
                     WVVVEVGRQPWVVQDYMPVSAAVTDISSSSVQLTFFIFAVVFTALLIAEVSIMTRQIK
                     IGPTKQEK"
     gene            complement(9664..9903)
                     /locus_tag="PLK75_08090"
     CDS             complement(9664..9903)
                     /locus_tag="PLK75_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOY39245.1"
                     /translation="MKLIRNIFRFYVDGFKNMPAWGRTAWAIIVLKGVIIFILIKFLL
                     FPNFLKKNFETDEERSDYVLEQLTKKADNYDVTER"
     gene            complement(10127..11209)
                     /gene="hydE"
                     /locus_tag="PLK75_08095"
     CDS             complement(10127..11209)
                     /gene="hydE"
                     /locus_tag="PLK75_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOY39246.1"
                     /translation="MTYFEEILKKENHTQEDIILMLQAEGNDYELLLKRAYSLKRKHI
                     GNKVFFRGLIELSNICSKNCMYCGIRKDNEKVDRYALTDEEVLSCARYAYEHKWGSIV
                     IQSGELSTDNFTSRVEKLIREIKSIGKGALGITLSCGEQSDDVFKRWFEAGAHRYLLR
                     IEASSKELYGKIHPQDENHSYQERIAALNRLKRIGYQTGTGVMIGLPFQTIEDLADDL
                     MFMRELDIDMCGMGPYIEHEDTPLYSFRKAIPMLEDRLLLTFRMIACLRIIMPDINIS
                     STTALQTIHPNGREMGLKAGANIIMPNITPVKYHGNYNLYKGKPQIPEETEDYIKSLE
                     EQIKNAGDKVCYNAWGDSVHYLNKKK"
     gene            complement(11213..12088)
                     /gene="folD"
                     /locus_tag="PLK75_08100"
     CDS             complement(11213..12088)
                     /gene="folD"
                     /locus_tag="PLK75_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOY39247.1"
                     /translation="MQIIDGKKISEDIYQELYAETEKIKESGARVPHLAAIIVGNNAA
                     SETYVANKEKACTKVGFDSTLIRYPENTSQPELLCKIQELNTDNGIDGLIVQLPLPAQ
                     FNEQQIIEAIHPEKDVDGFHPINLGKMVIGMPSYISATPLGIIELLKRYHIDTEGKHC
                     VVIGRSNIVGKPISILMSRNTYPGNATVTVCHSKTNNLKDICLSADILIVAIGKPNYL
                     KADMVANGAVVIDVGINRVPSDKTKSGFALVGDVAFDEVAPKCSFITPVPGGVGLMTV
                     ASLLLNTMKSYKNKQ"
     gene            complement(12092..13423)
                     /gene="ffh"
                     /locus_tag="PLK75_08105"
     CDS             complement(12092..13423)
                     /gene="ffh"
                     /locus_tag="PLK75_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOY39248.1"
                     /translation="MFDNLTDRLERSFKLLKGEGKITEVNVAETLKDIRRALLDADVN
                     YKTAKQFTEDVKDKAMGQNVLKAVKPGQMMIKIVHEELTALMGTEASEIRLASKPSII
                     LMSGLQGSGKTTFSGKLANMLKAKKGRRPLLVACDIYRPAAIEQLKVIAAQIEIPVYA
                     EPESKKPVEIAKASIQYAKQNNCDVVIIDTAGRLAIDELMMTEIKAIKDAVKPDEILF
                     VVDSMTGQDAVNTAKEFNDRLDFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KMEALDVFHPSRMATRILGMGDIVSLVEKAQEQFDVEESRKLQKKIARNEFNFDDFLK
                     QIQQIKKMGNFKDIASMIPGVGKALKNLDIDDNAFKGIEAIIHSMTPLERRSPKLING
                     SRRKRIAEGSGTSVQEINRLLKQFDETRKMMQMMTDKNNTRKLMGRMPQKR"
     gene            13566..14642
                     /locus_tag="PLK75_08110"
     CDS             13566..14642
                     /locus_tag="PLK75_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39249.1"
                     /translation="MKKHRVFKLIIWTVVVILLLVLVPASVLITNIVKDYKPDDKTMI
                     EENPSFDTIHIDSVSSFNLMSWNIGYCGLSNEMDFFYDGGTKVRPSKEKVFENYIEIQ
                     DFLQNDSITDFFLLQEVDNKSKRSYKMKEYDNLWGLKLLDGYSAYYAINYKVWFVPLP
                     ITNPMGKVESGLVSITRFKPKSVIRISYPSLEKFPMKYFMLDRCIMVIRCQLSNGKDL
                     TIINTHNSAFDDGSQRKLEMNMLKKYLLQEFEAGNYVIAGGDWNQVPPGFKPDFKKDS
                     FDPGYSIGAPDNFLPDGWTWAFDNRVPTNRKVDKAYSRGTTGTTVIDYFIVSPNLKVD
                     SIANLDMEFRHSDHNPVTLTVSFR"
     gene            complement(14925..>16277)
                     /locus_tag="PLK75_08115"
     CDS             complement(14925..>16277)
                     /locus_tag="PLK75_08115"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39250.1"
                     /translation="GCIEQTVSAAFPQLYLSYLINLDAKQKEKIQQYINQALNSLALY
                     QSGSGGFNYWPSAYSEVSSWGTSYAGQFMLEAEALGYQVPSQVKKKWLSYQKAKASGW
                     VDEGYSSQLDQAYRLYTLALAGNPDKGAMNRLLQNKNLRKESKWRLAAAYQLCGKKDV
                     ALQLIKGADTNVSDYYEMGGNFGSALRDKAMILETLVLLGEKQKAFELLLDISEQISK
                     DTWYSTQTTAYSLLAMSKYVAYVKPTGKIACKIAFNGKSTPVNCDKPVYKMQLPIHET
                     AANSFSLKNNGSSMVYYRLVFEGIPQYGNETAANSKINMKIDYTTLDGTPLDPAKIIQ
                     GTDFIATVQIQPEGNPTKYENLALSQLFPSGWEIINSRMFDTDLGSQSYASYQDIRDD
                     RIYTYFDINYRSVKTFKVMLNASYAGKFYMPPVQVAAMYDNTIYARTKGQWIEIETSY
                     "
BASE COUNT         4839 a         3235 c         3111 g         5092 t
ORIGIN      
        1 tgtattttag taacactgtt cttttacaaa accttgagaa gcaaaacagg accaagacaa
       61 agcttaaaca atatttaata ctgctcacaa gaatactcct gatagttgca ttagttacag
      121 cattcagtca gccctacata cctgtgaata aaaatcagaa tcagggaggc aagaaatata
      181 ctgcaatata tttagacaat tcttttagca tggatgggga aagtgaaaag ggtaaaatga
      241 tagatgcagc caggcagttg gcctacgata ttgttagtgc gtatccgccc gatatgcaat
      301 acttttttct gacaaaccat ttttcaccgg aatacctgca attgatgaac acaaacgaga
      361 taaaagaaaa tattgctgat gtgaatctga ccccgatgtt caggagtttg cctgaggtgc
      421 tggctcgttt ttcggcacta gccggtcagg atgctactct aaacatatac gttatcagcg
      481 atatgcagga atgtaatttt aaatcagata ttactttttc atcgttcagt catcatgttg
      541 tactagtgcc tattacaggt aatttgcagg ggaatctttc agtagattca tgctggttta
      601 atgctcctgt taaaaacatc gggaaagctg atgagttaac tgtacaaatt aaaaaccatt
      661 ccaatcagcc ttatgtcgat attccggtaa aacttagcat aaacgatacg gtgaaaacca
      721 tttcttcgct gaccatagaa gcaggagaaa cggtgaaact cccgcttact ttcacaaata
      781 catcctccgg taacattaaa ggggaggttg gtattgatga ttatcctgtt actttcgata
      841 acaacttatt tttttcatat tccattaata atgttgtaaa tattctgata atcaatgaaa
      901 cgggaacgaa ccaataccta agtgcactgt acaaaactgc tgacgataat tttaattaca
      961 cagaagtaaa agccggcagt gaaaagaata gcgaatttca acaatatcag ttgattattg
     1021 ctaacgggct gaaatcaatt ccaagcggtc tggctgagtc gttcaaagag tatatcgaaa
     1081 cagggcacag tttagtattg attccgggag agcaatgtga tctgggttcc tacaacagtt
     1141 ttctgagtgc attggaagtt ggaaaaatca gtgaggtatc gctgcagcaa cgtagaatat
     1201 cggaaatcaa ttatagaaac tatctcttta aagatgtatt cggaaaaatt gataagaata
     1261 ttgatttgcc tgatataaat agtttttatc ctttgcaggt tttctcaaat tcaaatgcag
     1321 atgttgtgct gttcgatgat aaatcgaatc ctctgattgc aacaaaccca acaggggcag
     1381 ggaagtttgt attttgcgca attccattaa gtaaaaccaa tgagcttttt atgaaacacc
     1441 ctttatttgt tccattattc tataatatgg ccttatattc tgggtcttcc caaagcttgt
     1501 actgtgttgc cggaagggaa tcgtatattg acataagcgg gttccgaccg gatcaaaata
     1561 ctgttttaca tatcgttgac tcactaactg atataattcc tgcgagcaga attattccgg
     1621 gcggtacccg tattacaatt cccgaaacac tggtgcgggc cggacactat agtgttacat
     1681 caggaaatgc cacacattcc cttatatcga tgaactactc tagagaagaa tcggcaacga
     1741 aataccttca gggaaacgaa attatcgagg aattgaaaca agcgggtatt gaaagtgtgt
     1801 caagtgttga ttacctgtcg gagaacttca ggtcaaatgt agcagagcaa agcttaggaa
     1861 ccatgctgtg gaaatatttt attatcctgg cattagtttt cgttgtcgca gaaattgcaa
     1921 taatccgact gatgaaataa taagaagctt agccggtttt actaattact cccggcgatg
     1981 aataataaac attctgtatt ggccggaact cgtttatcag caatgcccat ttcgaaggat
     2041 cttcttttat caggttcagg atttgtttac ggtatttttt tataaactca gtgattgcag
     2101 gtgcagaaac actgggttca caatttgccc caaccatttt gccggtgata ttatgaccct
     2161 ttgctaaaag tttttcaact gaacgcagaa gtttgtaaag gaattgctct gcttttagtt
     2221 ctttcgaaat agtacgggta acagaagtaa catggggggg ctggttattt ctatcggaag
     2281 attcgtttaa aaacaaaagt tcttcaccaa ttattaaagg cgttgaaggg tcggtagcag
     2341 ttatatttac cgcaaactga accgcatttg ccaggatatg gtttattttg tggatggctt
     2401 cacgctgctt ccacagtttt tctgctgcat catcagaaag cagaatttta cgattgtatt
     2461 gctgcgaaat accatccaag tattcttttg caagtcgttc aatgctttgt ttatgcattg
     2521 actgttccat acagacagct tctttgcttt tttttaccgt gtcaatgagc atcaaagtgt
     2581 tccggctgag atccctgtga agtctggcat cgctttcggc agcccttatt acagccatta
     2641 cactgttatg aagtagtaat ccaagaattt tttttgtctg aacctcgagc tttccatcag
     2701 cagggttaat tccaaatgcg cttaaatctt gtttcagctc aatgaaaacg agatcctgat
     2761 tgctgtcagc catactatca atgcgcaagc acataaccgt gtgctcgctt tcagagaaaa
     2821 tttcgagctg ggtaatgccg ctaatattat tttttattgg aacattgtcg ggtgtgaacc
     2881 attgaatcgg ggttttccgg ggctttcgca agtttgaaat aatattatct tcatcactaa
     2941 ttacattatc ttcaggttcg ttgcctcgaa aaaaccgcgt ataatgcagc aagttatctt
     3001 cctgactcaa ataaatacaa tatccacaaa cagcacccgg tgcaatcatc tttaaatgtt
     3061 gcagtgcaat aactgcatga ttccggttga agctgtaatt cacgtaatgc tagtattgaa
     3121 tgcacaaata taataattaa ttaattaaaa tgcaaatgct taattaaaaa attgcttaat
     3181 aatttaattg tttaacaaaa ccccttcagt tatgaattaa cttaattgtt tctgaaagtg
     3241 cttataaatc aaatatattt acatcaaaaa caaagctgtg aattatttaa taattatagt
     3301 atttagctat ttaattctgc aagtattaat tcgtattatt aaccatttaa ttaagtatga
     3361 tgatgataaa taaaacaaat ccactcattt tactgatctc agatcggtat attagcattc
     3421 atacagatgt tcaaaccaaa aggaattttt tgtttaataa aaatggcctc agtttaaaaa
     3481 agcttaacgg aataaaggcc tttgttcaaa aattacttga gtactaccga gaggaatgta
     3541 acaggcgtgg tctggtagtg tttgtcgata acacattcct agtacaaaaa gagcaatgga
     3601 tttatgataa agcctcttgt aagagccatg caataaaaaa gcaaacagaa aagaatcttc
     3661 cgggttttgt aaaagtacac cattcagaaa cctactcaaa gtcagggaat gtgagcagca
     3721 ttgaaaatca acagcgtgga gatttaatgt acagctttca ctggaataat gaatttgagt
     3781 cacttgtttt gcaggccata gccactgatg tttcaaacgt ggaattttac cccaaaagtt
     3841 cacttcaaca acataaatcc agtaataaaa gtgtaatggt tctcaatttt cgacttaaca
     3901 aagtattgct agaaaaatat tactgcgatg agttgattta ttcagatacg attggaacag
     3961 ggcacaatac aataattgag aatatagccc gtacattcgg tatatcgcaa cagcatgcag
     4021 caaagatgta tgtaatgtac ggttcggtgt tcctcgataa aagtcttaca gacctgttta
     4081 ttgatgtaaa gctgaatgaa tttcatgtaa acgaagtgcc ctatgcccaa ttatgctctc
     4141 tgattcaaga aggttatatt gatatgctga aaaaggtttc cgcaagactt tcctccaaaa
     4201 aagtaagtcc cgaatgcgat aggattatgg tttccggtaa aaatatcagt caaaaagtcc
     4261 cgcaactggt aagtttattt tttaatacag agtgtaatca ggtagttgat gctttcgatt
     4321 tttcatacaa cgagttggtt tcttttgtac ctgcaacaaa agaactacct ataatgacag
     4381 cagcagaagt tgaaatatgc gaaaatatta ttcctgagcc aaggaaaagc tcttttcaga
     4441 agaaatcgtt gcgtgtaatc aatgcgatta tgaatgaaat cagagagttc atgactgagc
     4501 cggaagctga gtttatttca tgaccagaat gaatccgcta ccgtgtacat tgttaatatc
     4561 aatactatcg atatccttaa aatattttcg aagtttggtt atgtaaacat ccatactgcg
     4621 ggcattaaaa taattgtcgt caccccagat gtgtttcagg gcaacattcc tgtcgagtac
     4681 atgattttta tttgcgtaca acattttaag aagatccgac tcccgcgggg taagcctgaa
     4741 ggtagagttg tttccggtaa gagtgcgcaa ttgatggtca aaaatgtaag gtccgatttc
     4801 aatcatgtta ggaatatcgt cgaagctttc tttgtttctg cgcaaaatag ctttcagttt
     4861 acgcagcaat acttccgaat cgaaaggctt tgtaagatag tcgtcggcac ccacttcaaa
     4921 gccacgtaga acatcttcct tcagcgattt ggcggttaaa aaaattaaag gaataccttc
     4981 gtcaatcttt cttatatcca ttgcaatact aaatccgtct ttattcggaa gcattacatc
     5041 gagtatgcaa aggtcgaaac tggtactcct gaaaacaggt acagctctca tgccatcgtt
     5101 aacaagcatt acctcgtaat cgtttatttc gaggtatgat ttcagcactg cgccaaaatt
     5161 gatgtcgtct tccacaagta ggattttgtc ttttttcata gtattctaat ttattggtag
     5221 gcttacgata aatgtacttc cctttgaatg attccctttc accgaaatat ttcctttatg
     5281 agcagcaaca atttccttaa catagtttaa ccccagtcca aagcctttaa tattatgtac
     5341 atttccggtg gaagctctgt agaatttgtc gaagaccctt ttcattacgt ccttactcat
     5401 gccaattcct ttgtcctcaa cttcaaaaac aaaatggttt ccggaattat aggtgcgaac
     5461 aagaatgtcg ggttggtcaa cagagtattt attggcatta tcgagaaggt ttcccacaac
     5521 attgagcaat gtgtcacggt cagcttttat aacaggaata tctgccagaa aatcggttct
     5581 gacatcaccg tttcgttgct gaatgataag ttcaatattg cttattgcat ctttaatgag
     5641 ttcatgcatg tcgacaggtt ctttcttcag gttcaggctt tccctgtcga gaagtgcaag
     5701 ctgaagtacc tgttctacat gctggttcat tctttgggtt tcctgattta tgatctcggt
     5761 gaacttatcc acagttttcg ggttttcacg tacgcgcggg ttgttaatcg acgctgcggc
     5821 cagttggatg gttgccaacg gagttttaaa ctcatgcgtc atgttgttga taaaatcgtt
     5881 cttaatctcg gtaagttttt tttgccgaaa aaaagctaca agagttattg caaaagtaat
     5941 gataagaata agtgaaagta ctaccgttga gattaactgg gcatatatac cctccaaaac
     6001 atactttctt ttccccggaa aataaatgta caatgcatca ggagcggaaa atattgcacc
     6061 ctgatataag ttaattttat acgcatcgtc agtaatactt tcgttatagc catcggtttt
     6121 aagcctgatg ttgtgattgg catcagccac agcaaattca aaattcagtg aaagcccacg
     6181 ctgtttcagt tcctcgctta tcgcttttct gatatattcc ttatcgaatc tgttctccag
     6241 agggcggcgc atttgtttgt attctttttc gagttcgctc acaacttcat caagggcgag
     6301 tccaccagta ttaaattttt catcgaaaaa acgctcaaaa gtggaatatg ataaaaactg
     6361 ctccgggtaa tagtcgttac cccatgtgtt cgaaaactcg taaacctgaa cgcccccata
     6421 cgatgaccgg atggaaatac taaaattatg aatcggtaat tcaggctggg gagggatata
     6481 cccaaaacga gacatattgg ggtcggcagg gtaggtgctg tcgttttgag cagatcttga
     6541 attcagcatt ttattataca gaaaaaccaa tgcatcacgc ttttcaagct taataagtgc
     6601 ctgcgacaaa gattccctca cacctctatc aaccagctct ttcttttcgt tgtacgatcg
     6661 gtaaatatga cctgcctgca tcgctataat ggttaaaagc gatacaatca tcagacctgc
     6721 cagcaggtat actgttcttt tctttttcat tattacaaaa gtaaccaaaa ccggacttca
     6781 ttcaatcgta tttaactatt cattaagata atgaacagta agaacttgcc atactgtttt
     6841 tatttattta tttctcctcg gcctcaacat tcgttttgtc ctcctgcttg tcgaaatgcc
     6901 gcaatgcggc cagtgaaatt aaataagcgg ctagtattaa tacaggccac atcaaaaggc
     6961 tgattatttc tttcgtgtac ataattgttg attttaatag tgatgctcat tgctgtcttc
     7021 tatttctgac cttgtgattt tacgtttgtt tattgaacgc caggccacaa cgatataggc
     7081 aaaaacaaac ggaatgatta acgaaactat tgccattgtt ttgagtgtgt actcactgct
     7141 gcttgcgtta tacactgtga gagaactctc agggtgtgta atggatgggt aaaacgaagt
     7201 gttattaagt cctgcaataa gcaacagtgc aagaacggca aaaaaagtac ctaagccggt
     7261 aaaccaaata ccgttatcat atttgtctga aaccacagtt gctattatac cccatagaac
     7321 tgcaagcact cctgcaataa acattattgc aaccaagggc atttgaatga ggttgtggag
     7381 gtatttgtac ttttcaaaaa caatgctttt tgtttcaggg tcaatagcat atcccgtttt
     7441 cagcaataaa atggtaacaa atgaaacaaa tagtaaaaca aacggtacag cattgattat
     7501 caattgtttc tttattctgt tacaaatttc attattttca attgttttac gaaaatatag
     7561 taatgctaga accctggaca agaaaaaaac gctgaatcca agcaacaggt tatgataatt
     7621 caacgctgct tccaatcctc tggcagggtt gtcccatttt acgtggttca ttgtgttcaa
     7681 agagaattca gcgccattat acagggtagc tactacaata ccaagcaaaa ttgtgcccag
     7741 aaatccatta atgaacaata gtgtttcgaa ggtttttttt ccgaatacat tcgatggttt
     7801 gcttctgaat tcgtatgaaa ccgcctgaac tgtaaatgca agcagaataa tcagccacac
     7861 ccagtatgct ccgccaaaac ttgtgctgta gaataacggg aacgatgcaa aaaatgcacc
     7921 tccaaaggtc accagtgtgg tgaaagtgaa ttcccattta cgcccaaggc tgtttacaag
     7981 catacttcgt tctgtttcat tttttccaag ggtataaatg agcatttgcc ctccctgtac
     8041 aaaaagtaaa aatactaaag cgcttcccag taccgaaacc agaaaccacc agtaatgctg
     8101 aagtgcagca aatgttattg aagccataat tatttttcct gttttgttgg accaatttta
     8161 atttgtcggg tcataatgct gacctctgcg ataagcagtg ccgtaaatac tacggcaaaa
     8221 ataaagaatg taagttgtac agatgagctc gaaatatcgg tgactgcggc tgatacaggc
     8281 atataatcct gaacaaccca cggttgcctg cccacctcaa caacaaccca tcctgcttct
     8341 gatgcaataa aactgagggg aatacacagt agggcgatat aatacagaag ctttcggaac
     8401 cggaattttt cgatggtatt tctgaatgaa aaccaaaaga acgccagaaa tacaaaaaca
     8461 aaaaaagtac ccaataatac cattgcatgg aagctgtaga aaaccaactg aacattgggt
     8521 attatatccg taggtttatc gaggaaaccg tatccgaaat gcttgtagtt ctcgttaaac
     8581 aatgcgagca tttttcgtgc ttcagcagta ttgccgtttt tcttggcgat tttataggct
     8641 tttagcgatg atactgcaat tttaccttta tcgattcgct cctgagccgg taaaatacct
     8701 ttttcgggaa caccattgat taaatcttca accccgggga catagtggtc tttattgaat
     8761 gcaataagat aagaaagcat tcctttaaac ccgaatttaa caacgtaatc atttttaccg
     8821 tcgaaagcct gtttgccggg tgtaataata ccaaatccca taagttcggc gtttgtaccg
     8881 cctttataaa gaccttccat tgcagcgagt ttcataggct gcttttcggc aacctgcaca
     8941 gctgagccat gtccggtaag aataattgct atgctcgata ttagcccgaa aacagatgca
     9001 attattatgc tcctttttgc cagcaacgat tctctgcccc gtaacaaaaa ataggaacta
     9061 atgcctatta caaataaaga agcaacgata tagccgcttg tgatggtgtg aagaaacttg
     9121 tttacagcaa ccggcgatag aaaaacatcc cagaaattaa ccatttcgtt tcgtgcagta
     9181 tccgggttaa aatacatacc tgtcggaaac tgcatccatc cgtttgcaac cagtatccaa
     9241 agtgctgaga gattggctcc aattgctgta agccacgttg atgcaaggtg aaatttctta
     9301 ctcactttgt tccagccgaa gaacattaca gcaataaaag tagactccat aaaaaacgcc
     9361 atgatgcctt caatagcaag aggtgcccca aaaatatcac ccacaaacca ggaataattc
     9421 gaccagtttg tgccaaattc aaactccaga ataataccgg ttgctacacc aattgcgaag
     9481 tttatcccaa atagcttcat ccaaaatttt gtagttcttt tccattccgg atctcctgtt
     9541 tttacataaa tagtctccat gaaagctatg ataaacaaaa tacccaaggt aaccggaaca
     9601 aaaatccagt ggtacatggc agtaagtgca aattgtgctc tcgaccagtc gactaaggaa
     9661 gggtcaacgt tcagtaacat cataattatc ggcctttttt gttagttgtt ctaatacata
     9721 atcgcttctt tcttcatctg tttcgaagtt cttcttcagg aaatttggaa aaagtagaaa
     9781 tttaatcagt ataaaaatta ttaccccttt caagacaata attgcccagg ctgttcttcc
     9841 ccatgccggc atgtttttga aaccgtcaac atagaatctg aaaatattgc gaatcagttt
     9901 catgcagtca aattagtaaa aaaataatgt aaacaaatcg ttacgatgcg aaaatttttc
     9961 aaaaattatt aatacgacgt cttttcaaaa tcatcggaat ttagctctcg ttaacggcgt
    10021 ttaaaagtat tgtatttggg ggtaatcaag cttgaaaatt tacaaataat atattgaata
    10081 acatatttaa ataagcattg ataattaatt ataatataat cagttattat ttctttttat
    10141 ttaaatagtg tactgagtcg ccccaagcat tatagcacac tttgtcacct gcatttttta
    10201 tctgctcctc aaggctttta atataatctt ctgtttcttc gggaatttgg ggttttcctt
    10261 tgtacaggtt gtagtttcca tggtatttta caggggtaat gttaggcatt ataatgtttg
    10321 ccccggcttt caatcccatt tcgcgcccgt tgggatgtat ggtttgcagc gcagtagtgg
    10381 acgaaatgtt tatatcgggc ataataattc taaggcatgc aatcatcctg aaagtaagta
    10441 gcaatctatc ctcaagcatg ggaattgcct ttcggaatga gtacaatggt gtgtcttcat
    10501 gttcgatata ggggcccatt ccgcacatat caatatccaa ttcacgcata aacatcaaat
    10561 catcggcgag gtcttcgatg gtttggaacg gaaggccaat cataacgccg gtgccggtct
    10621 gatatccaat tctcttcaac cggttcagtg ctgcaattct ttcctgatag ctgtggttct
    10681 catcctgtgg gtgaatttta ccgtaaagtt ctttcgagga ggcttctatg cgcagtaaat
    10741 accggtgtgc cccggcttcg aaccatcttt taaacacatc atcactttgc tcaccgcacg
    10801 aaagagtaat acccaatgcg cctttgccga ttgattttat ttcgcgtata agtttctcaa
    10861 ccctcgaggt aaaattatcg gttgaaagct ctcccgactg aataacaatc gatccccatt
    10921 tatgctcgta ggcataacgt gcgcacgaca gcacttcttc atcagtgagt gcatatcggt
    10981 cgactttttc attgtccttc ctgataccac aatacatgca gttcttagaa caaatattcg
    11041 atagctcaat cagacctcta aaaaacacct tattcccaat atgttttcgt ttaagcgaat
    11101 atgccctttt taaaagaagt tcgtaatcgt tcccttctgc ctgaagcata agaattatgt
    11161 cttcctgtgt gtggttttct tttttaagga tttcttcgaa ataggtcata attcattgct
    11221 tgtttttata tgatttcatt gtattaagca ggagagaggc aaccgtcatc aatccgacac
    11281 cgcccggaac gggtgtgata aagctgcatt tgggagctac ttcatcaaag gctacatcac
    11341 caaccagagc aaaacccgat tttgttttat cgctgggcac cctgttaatt ccaacgtcaa
    11401 ttaccacagc tccattggcc accatatccg ctttaagata attgggtttg cctatggcta
    11461 ctatcagtat atcagcactt aagcaaatgt cttttaaatt attagtttta ctatgacata
    11521 cagttacggt tgcattccca gggtaagtat tccggctcat cagaatgctt attggtttac
    11581 caacgatatt acttctgcca attacaacac aatgtttgcc ttccgtatcg atatgatatc
    11641 tcttaagtaa ttcgataatt cccagaggtg ttgcagagat ataggaaggc ataccgatta
    11701 ccattttgcc aaggtttatg gggtggaacc catcaacatc cttttcgggg tgaatggctt
    11761 caattatctg ttgttcattg aattgagcag gcaggggtag ctgtacaatt aaaccatcga
    11821 taccgttatc cgtatttagt tcttgtattt tacaaagcaa ttcaggttga ctggtgtttt
    11881 cagggtagcg tattaacgtg gaatcgaacc ctacttttgt gcatgctttt tccttatttg
    11941 caacataagt ttcgctggca gcattatttc cgacaatgat tgctgcaagg tgaggcactc
    12001 ttgctcctga ttctttaatc ttttcggttt cggcatatag ttcctgataa atatcttccg
    12061 agattttctt tccgtcaata atttgcataa tctatctttt ttgaggcatt ctacccatga
    12121 gttttcgcgt gttgttttta tctgtcatca tttgcatcat cttgcgggtt tcgtcaaatt
    12181 gcttgagcaa acggtttatt tcctgcaccg atgttccact accttcagca attcgtttgc
    12241 gtctgctgcc gttaataagt ttcggactcc tgcgttcaag cggtgtcatg ctatgaataa
    12301 tggcttcaat gcctttgaac gcattgtcat caatatcaag gtttttcaat gccttgccaa
    12361 ctcccggaat catgctggct atatccttga aatttcccat ttttttaatt tgctgaattt
    12421 gctttaagaa gtcgtcgaaa ttgaattcgt tccttgcgat tttcttttga agttttctcg
    12481 attcttcaac atcgaattgt tcctgtgctt tttctaccag cgaaacaatg tcgcccatgc
    12541 ccaagatacg ggttgccata cgacttggat ggaaaacatc gagggcttcc attttctctc
    12601 cagttccaac aaatttaata ggtttattaa caacgctacg aatcgagagg gcagcaccac
    12661 cgcgtgtatc cccgtcgagt ttagtaagca ccacaccatc aaaatcgaga cgatcattaa
    12721 actccttggc tgtatttacc gcgtcctgac cagtcattga atctactaca aacaatatct
    12781 cgtcgggttt aacagcgtct ttaatagctt taatttctgt catcatcagt tcatcgatag
    12841 caagccgccc tgcagtatcg ataataacta catcgcagtt gttctgcttt gcgtattgaa
    12901 tggatgcctt ggcaatctca acaggctttt tactttcggg ttcagcataa actgggattt
    12961 caatttgcgc agcgataact ttcagctgct caattgcagc tgggcggtaa atatcgcagg
    13021 caacaagcag cgggcggcgg cctttttttg ctttaagcat gttggcaagt ttccctgaaa
    13081 aagtagtttt ccctgatccc tgtaatcccg acattaatat aatagatggt tttgaggcta
    13141 ggcgtatttc ggaagcttcg gttcccataa gggcagtaag ctcttcatgg acaattttaa
    13201 tcatcatctg ccctggcttc actgctttca gcacattctg ccccatggct ttatccttga
    13261 catcctccgt aaactgtttt gcagttttat agtttacgtc agcatcgagt aaagcacgcc
    13321 taatatcttt aagcgtttcg gcgacgttta cttcagtaat tttaccttca ccttttaata
    13381 acttaaatga tcgctccagc cgatcggtca aattgtcgaa catcgttttt tatttttagt
    13441 ccagcaaaat tataaaaaaa cttcatccct aggaaacgat ttcaacaaag gcatcttaat
    13501 caaaactaaa aatttgaatt tttatctgaa attatatttg taattttgta taaattaatt
    13561 tttgattgaa gaagcataga gtttttaagc ttattatatg gacagtagta gtaattcttc
    13621 tgcttgtcct tgttcctgca tcggtactaa taacaaacat agttaaagat tacaaacctg
    13681 acgataaaac catgatcgaa gaaaacccga gcttcgatac cattcatatc gattcagttt
    13741 cgtcatttaa tttgatgagc tggaatattg gatattgtgg cttaagcaat gagatggact
    13801 ttttttatga cggtggcaca aaagtaaggc cttcaaaaga aaaagttttt gaaaattata
    13861 tcgaaataca ggattttctt cagaacgaca gcattaccga tttttttctc ctgcaggagg
    13921 tcgataataa gtcgaagcgt agttataaaa tgaaggaata cgataattta tgggggctga
    13981 aattgctgga tggctatagt gcatattacg caattaacta taaagtttgg tttgtgccac
    14041 tgcctataac aaatcctatg ggaaaagttg agagtggttt ggtttctatt acgcgtttta
    14101 aacccaagtc ggttatcaga atttcttacc ccagcctcga aaaatttccc atgaagtatt
    14161 ttatgcttga cagatgcatt atggtaattc gttgccaatt aagtaatggg aaagacctga
    14221 caataataaa cacacacaat tcagcattcg acgatggatc gcaacgaaaa cttgagatga
    14281 atatgctgaa aaaataccta ttgcaggaat ttgaggcagg gaattacgta atagccggag
    14341 gcgactggaa tcaggtgcca cccggtttta aacctgattt taaaaaagac agctttgatc
    14401 cagggtattc gataggggcc cctgataact ttctgccgga tggttggact tgggcattcg
    14461 acaatcgagt tccaactaac cgtaaagtag acaaagcata ttcaaggggt actaccggta
    14521 caacagttat tgattacttc attgtttccc cgaacttaaa agttgacagc attgccaacc
    14581 ttgatatgga atttcgtcat tccgatcata accctgtaac gctcactgtt agttttcgct
    14641 aaaacaataa tagcctacct gaggactaat tattggggta ataaaagcaa tttaatgtgt
    14701 cgaggtgttt ggaatcagaa tacaggagac agaatgcaga atacagaagg ggcgattgcc
    14761 aattgtaaag cattcatctc tatttggatt ttccatcctt tatcgtatca taagatttcc
    14821 atcccacaac gaaaagcacc ttctgtattc tgcattctgt attccccaaa gtaacaatat
    14881 gtcattggta ataagctact attgttgcga tatgtcaaac caaatcagta tgaggtctca
    14941 atctcaatcc attgcccttt tgttcttgca taaattgtgt tgtcgtacat agcagctacc
    15001 tgaacagggg gcatgtagaa tttaccggca tagctggcat tgagcattac cttaaacgtt
    15061 ttcacacttc tgtagttgat atcgaagtag gtatagattc tgtcgtcgcg gatatcctga
    15121 tagctagcgt atgattgact tcccaaatcg gtgtcgaaca tcctagaatt tattatttcc
    15181 cagcctgagg ggaataattg cgaaagcgcc aggttttcgt atttggttgg gttgccttca
    15241 ggttgtattt ggacggttgc aataaaatcg gtcccttgta ttatctttgc agggtctaat
    15301 ggagtgccgt caagggttgt ataatcaatt ttcatattga ttttagaatt ggcagcagtt
    15361 tcgttaccgt attgcggaat cccttcaaat accagtctgt aatataccat cgaactgcca
    15421 ttattcttga gagaaaaact atttgctgct gtttcatgaa ttggtaactg cattttgtac
    15481 acaggtttat cgcaattaac cggtgtcgat ttgccattaa aagcaatttt acacgctatt
    15541 tttcctgtag gcttaacata ggcaacatac ttcgacattg ccagtaaaga atacgctgtg
    15601 gtttgcgtac tgtaccatgt atccttgctt atttgttctg aaatatcaag tagcagctca
    15661 aaagctttct gcttttcacc caaaagaacg agggtttcta gtatcattgc cttgtcgcgc
    15721 aatgcggatc caaagtttcc acccatttcg taataatcgc ttacattggt atcggcacct
    15781 ttaattaatt gcaaggccac atcttttttg ccacatagct ggtatgcagc tgcaaggcgc
    15841 catttcgatt cttttctgag gtttttattt tgcaagagcc tgttcatagc tcctttgtcg
    15901 gggttgcctg ccagtgctaa tgtatataag cgatacgcct gatcaagctg ggaactatat
    15961 ccctcatcaa cccaacccga tgcctttgct ttctggtacg acagccattt tttcttaact
    16021 tgagaaggaa cctgatagcc cagagcctcg gcttcgagca tgaattgacc cgcataactg
    16081 gtgccccagg agctaacttc ggaatatgca ctcggccagt aattaaatcc gcctgacccc
    16141 gactggtaaa gagctagaga attcaaagcc tgattgatat actgctgaat tttttctttt
    16201 tgcttggcat caagatttat aagataggag agatacaact gtggaaatgc tgcagaaaca
    16261 gtttgctcaa tacagcc
//
LOCUS       DAOOQP010000069        16247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4350_length_16247_cov_4.641798, whole genome shotgun sequence.
ACCESSION   DAOOQP010000069 DAOOQP010000000
VERSION     DAOOQP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4350_length_16247_cov_4.641798"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLK75_08120"
     CDS             <1..1158
                     /locus_tag="PLK75_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOY39251.1"
                     /translation="TRVLDELSGEELFEGGVQLGEGLGKLFIETGKPFGKELISSFPE
                     QIPDHRILAIAQFLRDKNTDRQVVLVTKDINLRVKAKSLGLTAQDYKNDKVANLDNIR
                     KQVAIIEGADKNLISSLYDHPEGIPLEDFNFGIDPIAHQYFVLRNGSNSVLAHYDPFK
                     NVIDRVVKTAAYGIEPRNAEQAFAIDALLRPEIQLVALTGKAGTGKTLLALAAAIHMR
                     KSYLQIFLARPIVSLSNKDIGYLPGDAKEKINPYMQPLFDNLSVIKHKFSQQSADFKR
                     IEEMQKSEKLLIEPLAYIRGRSLSRVFFIIDEAQNLTPHEVKTIITRAGEGTKIVFTG
                     DIQQIDSPYLDSVSNGLSYLAEKMRGQDIFAHINLIKGERSYLAELASDLL"
     gene            complement(1164..3605)
                     /gene="pheT"
                     /locus_tag="PLK75_08125"
     CDS             complement(1164..3605)
                     /gene="pheT"
                     /locus_tag="PLK75_08125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOY39252.1"
                     /translation="MEISYSWLKDYIQFEESPEQLAEILTNLGLEVSSVTKFQSIEGG
                     LEDLFVGEVVSCIPHPNSDHLSLTKVNIGSSNLLDIVCGAPNVAKGQKVVVATVGATL
                     YGMAEPLTIKKSKIRGELSEGMICAEDEIGIGKSHAGIMVLPEDTPVGMKAAALFGIE
                     TDYVLEVDITPNRIDSASHFGVARDLAAFFAKTREVVCRLPEVPQLPYPSTPSEITVH
                     IENIEACPRYMGLYIKNVSISPSPQWLQNRLKAIGLKPINNVVDISNYVMFETGQPLH
                     MFDAEKISGGKVIIKTLPENTNFVTLDGIERKLSCNDLMICNSSEGMCIAGVFGGMDA
                     GISDTTKNVFIESAYFNPSWVRRTAKRHAISTDSSFRFERGADPNMAPYALKRAADLV
                     VQLAGGKIASDIIDQYPQPITPVTVNCLYKHFERLCGVELTKELIHSILKSLEIEITS
                     VNESGFSAIVPTYRVDVTREADIIEDFLRVYGYNNVTLPSKMVSSLTFTQKPDRTAIE
                     ANIAEMLTGRGFSEIMCTSFTRKRYFELFPDKKPVVEIHNALSNELNCMRPTLLFGGL
                     ESIARNISFQQNDILIYELGRRYTFNPDKDPVPGKGYCETNTIAIWMTGNLFNGGWNT
                     SAIKTDFFTVKAVTNMVLQRIGITPDDITADDFTDNKFETALRYTINGLEIAEFGIID
                     RKVLKETDIAQSVYYSEMNTDNALKIIKNRRTKAEPLPRYPKVKRDLALLIDEDIKYS
                     SIRDLALRTERKILRKVELFDVYKGKGIPEGKKSYALSFILQDSEKTLNDKEIERVMQ
                     KLMAAFIRELQAQVR"
     gene            3811..5271
                     /locus_tag="PLK75_08130"
     CDS             3811..5271
                     /locus_tag="PLK75_08130"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOY39253.1"
                     /translation="MIRTREDYLRSLKAMRPNIYKNGELIQDVTVHPATKRTVESHSR
                     AFDAANDEKYRALFTTVSYLTGKPIMRFNSLMKSLDDIMNNAKLKREMYHFTGSCSGG
                     VCVGWNAQNVMWAVTSDVDTEFGTNYHQRLEKWILQAEERGITCAGALTDAKGNRTLK
                     PSQQENPDTNVRIVEKRNDGIVVRGAKVMICGAAASEEIFVLPGSSYKDTDADYAVSF
                     VVPRDIEGLTLVETRRPSDGREYEEGWDVPDTGISQAYLIFDNVFVPNERVFMAGEFK
                     YTGKVIEYFTANYRACIGACVAGQGDVMIGAAVLMARANGISNKKFDDKFTVMAANNE
                     TTFGLGIGAMALGGPHPSGPWFAESKIAHLNKIYVATLPYETKRLCQEIGGGIVETGC
                     TPSYADFKNLIYGNTLLNLVKAGPCSSESRLRATRLSEWLTIGAGVPGCMHGGGSPDG
                     ARMVVKAFTPFEKYVEMAKKIAGIEEDIPDPAAVAK"
     gene            5338..6480
                     /locus_tag="PLK75_08135"
     CDS             5338..6480
                     /locus_tag="PLK75_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOY39254.1"
                     /translation="MFQFFNEDQKMLQKVVRDFVEQEIAPYAAEWDDKDTCPVELFKK
                     MGEMGMLGVFVPAQYGGAGLGLTERNIILEEVSRHSAGFGIAMMTHELAIAAIVNFGS
                     EEQKLTYLSPLISGEKIGGLSVTEPGGGSDFGNQMATIEKTDTAYVINGRKCFITNSH
                     NAEINVVTGTCGVNEKGRKLISAIIVPPGTPGWNAGRKEHKLGLRGSVTGDIICDNIV
                     VPHNCIIGKDGDGSKIAMHTIGHFGRSGMSAIAVGVLRGCLEEGVKFAKERIIYGKPL
                     SNLQSIQFIIAENKVDYDAAHAMLYNASAIYDRKQECVSDIAAVKLFSSEAAVRASKR
                     TIDLMGGYGIINEYPVGRFLRDAMAIIPSGGTSHIMSVIIASGLTR"
     gene            6540..7340
                     /locus_tag="PLK75_08140"
     CDS             6540..7340
                     /locus_tag="PLK75_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOY39255.1"
                     /translation="MPLQIAVCIKPVPDSKYYDKISIDPKTKTWDRSGIPMVIGDLDK
                     NALEAALVLREKFQGKISVFSMAPEFTREVMVQALAMGADEAFLLSDRSFAGADTLAT
                     SYTLASAIKKAGNFDLVLTGNESDDGSTGQVSPQLGEWLGFPHLMNVVHLDFDGELLY
                     VDTLAETGIYHYQVKLPAVIAVTRKVNKPRLIGVMGLMKAKAKPLNLWTAADLGADAN
                     RLGLKGSPTQPGDMVSPDTNRKAERIDGSPEDIADRIIVELRKAGIIG"
     gene            7343..8368
                     /locus_tag="PLK75_08145"
     CDS             7343..8368
                     /locus_tag="PLK75_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOY39256.1"
                     /translation="MESSDNTYRILVFAEIIDNTIARNSLELLGEANRMKKCIKKSVE
                     VIAAFIGNDVHAFADMAIKYGADTCVIFNNTNLEHYRSDCYGDALAKIVKKLQPEIVL
                     IGATFFGSEIAPTVAAKLQTGLAAHCTELKIDNNERFIQVVPAFGGKVLGEIITPVHR
                     PQMASVKPGIMTSLYDEKRTGQIAYFSEELSDFGDRIKFAGITPVKASGKPLHEADCI
                     IGGGYGIGSRENWEVLTKIAALLNGATGCTRPALDEGWTTGEQTMIGTSGVAVRPKVY
                     INAGISGAAHHTCGIKEAGLVISINKDENAPIFNSSDIKIVADWKKVLPVLLEKLNQL
                     NDAGSLK"
     gene            complement(8421..8999)
                     /locus_tag="PLK75_08150"
     CDS             complement(8421..8999)
                     /locus_tag="PLK75_08150"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOY39257.1"
                     /translation="MITLPELPYAMNALEPYISEQTVKFHYTKHHQAYVNKVNALIAG
                     TNFEGKSLEEIVKKSEGGIFNNGAQVWNHTFYWEGFGKNKGGVPVGQLAKAIDTQFGS
                     FDSFKEQFANAAANQFGSGWAWLVVNQQGKLEILATGNANTPLRDGYKPLLTCDVWEH
                     AYYLDYQNLRPDYIRNFWEIIDWEKINTRFLQ"
     gene            complement(9124..9906)
                     /locus_tag="PLK75_08155"
     CDS             complement(9124..9906)
                     /locus_tag="PLK75_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOY39258.1"
                     /translation="MKYGITEIAIVPLRSSPSEGSEMLSQLLFGEWFEVLIERSNWTK
                     VKLFHDGYEGWIDTKTISFITHEKYEQLCSDKKVSVVSEPFLEIENQKHERFYLPGGT
                     TLPYHNGNQFEVSGNNFTIIGKTPVKFTATDNQLPVVLAMQYLNSPYLWGGRTPWGID
                     CSGLTQTVYRILGISIPRDASKQVQAGTTVNLLSEAKPGDLAFFDDNNGHIKHVGIIA
                     ENGKIVHASGKVRIDNIDHNGIFNTDRHIYTHNLRVIKRIIQ"
     gene            complement(9949..10470)
                     /locus_tag="PLK75_08160"
     CDS             complement(9949..10470)
                     /locus_tag="PLK75_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOY39259.1"
                     /translation="MKPILKYTVFILTGLMVIFACNPKPKRPDVSNNKELKQVVYNFD
                     NPPKFVKDGELVFIDKGSLRDIATFDIELASTFEKRAMGLMYRKEMKPNQGMLFIFDN
                     EEVQSFWMRNTLIPLDMIFVNAHKEIVTIHKNTPVLSDESFASDKPALYVIELNAGTC
                     DSLNIKKGDLINY"
     gene            complement(10473..>11658)
                     /locus_tag="PLK75_08165"
     CDS             complement(10473..>11658)
                     /locus_tag="PLK75_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY39260.1"
                     /translation="RKTSMIGQQQMGQMLSQIEEMITGLRIIKAFNAENHIRRKFIHG
                     NQEYTNLMNKINRRRFLASPLSEFLATVAIVFVMWYGGKMVLQNTSGLSSQAFIGYLI
                     IFSQIITPAKAFSTAWYNIKKGLASLDRVNEIMNSEERITEKPNAVSINSFNNQINYT
                     SVWFRYNETHEVLKNINITIEKGKTIALVGQSGSGKSTLVDLLPRFYDIRQGIISIDG
                     NEIKDLKIKDLRKLIGYVNQTPILFNDSFYNNIAFGIENTSMEDVVAAAKVANAHDFI
                     IETQHGYNSNIGDGGCKLSGGQKQRLSIARAVLKNPPILILDEATSALDTESEKLVQE
                     ALENLMKNRTSIVIAHRLSTVKKADEIFVIHDGEIVESGKHNDLIDKNGLYTKLYTLQ
                     MS"
     assembly_gap    11659..11668
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11669..12291)
                     /locus_tag="PLK75_08170"
                     /pseudo
     CDS             complement(11669..12291)
                     /locus_tag="PLK75_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            complement(12312..13655)
                     /gene="purB"
                     /locus_tag="PLK75_08175"
     CDS             complement(12312..13655)
                     /gene="purB"
                     /locus_tag="PLK75_08175"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOY39261.1"
                     /translation="MNTFSLYAVSPIDGRYRKQVESLSAYFSEYSLIRYRLHTEVEYF
                     IALCEIPVPVLADFPNAGFQIMRNWVAGFSESDAIRVKEIESVTNHDVKAIEYLLKEK
                     FDQQGWGKYRELVHFGLTSQDINNTAIPAQLRDFIQEQYAPALQMLIDNINHLSEQWN
                     DVPMLAHTHGQPASPTRLGKEFRVFSARLETQFQQLKAIPFSAKFGGATGNLNAHYVS
                     YPNIDWHCFAERFVTKKLYLSRSYPTTQIEHYDNLAAICNTLGRINTILIDFARDMWT
                     YISMEYFRQKIKTSEVGSSAMPHKVNPIDFENAEGNLGMANTIFTHLAEKLPVSRLQR
                     DLTDSTVLRNLGVPSAHTIIALNSLQKGLGKLLLNEDKISADLASNWAVVAEAIQSIL
                     RRENYPEPYETLKALTRKNNVVSKEDIASFIKALNVDENIKNELLQITPYNYIGK"
     gene            13755..14522
                     /locus_tag="PLK75_08180"
     CDS             13755..14522
                     /locus_tag="PLK75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOY39262.1"
                     /translation="MKIAVVSYLNTIPFRYAIEKSPVKPVGVEYIYCPPSECASLLIN
                     NKAQGGLIPVAAIPSLKKHYVFSDYCISAYREAYSVVLLSQMPLSEIKTVILDYQSRS
                     SVAMFKLLNQHYLHFNFDFVQGQENFEKQICGTTAGLVIGDRAMKYRMHAEYVYDLAE
                     MWFEHTGLPAVFALWVSTVPVASQIKLAFDSMMQFGMKSVDEAIHYFNPAYDRELMHS
                     YLTENLCYSLDSNRRNSVNLFLSLLNNGDFKTNFSFQ"
     gene            14519..14971
                     /locus_tag="PLK75_08185"
     CDS             14519..14971
                     /locus_tag="PLK75_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY39263.1"
                     /translation="MNKFMYSVRNYKSDDYQDVENIWNACDMGGSVRGDTATIVERTL
                     AGGGALLLLVASDGENETIIGTTWITNDARRLYLHHCGILPEFRGLGLSKLLVQEALL
                     MAKRLGMQIKLEVHRNNTVAKALYAKKYFTYLGDYEVHIVRDVSKIPD"
     gene            14972..>16247
                     /locus_tag="PLK75_08190"
     CDS             14972..>16247
                     /locus_tag="PLK75_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOY39264.1"
                     /translation="MKSLHNDILRLAVPNILTNLTVPLLGLVDIGLMGHLTDAVYLGA
                     VALGSTIFSLLYSGLLFLRMSITGFTAQAYGAGYHRESSLVLGRGVFLALIMGVMLIL
                     AQHPIEWGLFKLVNASDAAERIAVDYFRVRIWAAPATLCVFVFTGWFLGMQNSRIPLL
                     IAVVINVVNILLSILFVNRFGMNAAGVALGTVIAQYCGLLISVLCFLKYYGRFRLLFS
                     LSGMIRMDRLTRFFKVNGDILVRSILITGTFFYFNAVSASMGNLVLSVNSILLQFLYI
                     FSYFIDGFAFAAESLTGKMVGSGDNSGLRKLVKTLFLWSGILSLAFSLSYLVFGNDFI
                     SLLTDNSEIVSQVKPFFFWAVLIPVVSFAAFVWDGIYIGATASRYLRNAMLIVVLVFF
                     LPASYFMKDAWGNHGLWLSLIIFLSARGVMLGL"
BASE COUNT         4549 a         3237 c         3478 g         4973 t
ORIGIN      
        1 acacgtgtat tggatgagct ctccggggag gaattgtttg aaggaggcgt tcaattggga
       61 gaaggattgg gaaaattgtt tatcgaaact gggaaaccct ttggtaagga gcttattagc
      121 tcattcccgg agcagatacc cgatcataga attttagcca tagcccaatt cctcagagat
      181 aaaaacaccg atcgtcaggt ggtgctggtt accaaagata ttaacctcag agtgaaggca
      241 aagtcgctgg gattaactgc gcaggattat aagaacgata aggttgcgaa cctcgataac
      301 atccgcaaac aagttgccat tattgaagga gccgacaaaa acctcatttc tagcctctac
      361 gaccatccgg aaggcatacc cctcgaagat tttaattttg gtattgaccc cattgctcac
      421 cagtattttg ttttgcgaaa cgggtcgaac agtgtattag cccattacga tccatttaaa
      481 aatgtgattg accgggttgt gaaaacggca gcctatggta ttgagccacg aaatgctgag
      541 caggcatttg caatagatgc cctgctccgc cccgaaattc aattggttgc ccttaccgga
      601 aaagccggaa ccggtaaaac gcttctggcc ttggcggctg caatacatat gcgtaaaagc
      661 tatttgcaga tcttccttgc aaggccgatt gtgtcgcttt caaataagga tattggatac
      721 ctccctggtg atgctaaaga aaaaattaac ccatatatgc agcccttgtt cgataatttg
      781 tcggtgatta agcataaatt tagtcagcag agtgctgatt ttaaaagaat cgaagagatg
      841 cagaaaagcg aaaaattgct tatcgaaccg cttgcctaca tcagggggcg cagtctttca
      901 agggtgtttt ttatcatcga tgaggctcaa aaccttactc cccatgaggt gaaaactata
      961 attacacgtg caggtgaagg aacaaaaatc gtatttaccg gcgacattca gcaaatcgat
     1021 tcgccatatc tcgactctgt ctccaacgga ttatcgtatc ttgccgaaaa aatgcgcggt
     1081 caggacatct ttgcacatat caatcttatt aaaggtgaaa ggagttacct cgctgagttg
     1141 gccagcgatt tactataatc agtttaacgc acctgcgctt gcaattccct tataaaagct
     1201 gccatcagct tctgcattac cctttcaatc tctttgtcat taagagtttt ctcagaatcc
     1261 tgtaaaatga aactaagtgc atacgatttt ttaccttcag gaattccttt gcccttatat
     1321 acatcgaaca actcaacttt tcgtaatatc ttacgttctg tccgcaatgc caggtcacgg
     1381 attgagctgt actttatgtc ttcgtcaatc aggagtgcaa ggtcgcgttt tactttagga
     1441 tatctgggca gtggttctgc ttttgttctg cgatttttga tgatttttag tgcattgtca
     1501 gtattcatct ccgaataata cacactctga gctatatcgg tttctttcag cactttcctg
     1561 tcgatgatac caaattcagc aatctcaagt ccattgatgg tgtaacgcaa tgctgtttcg
     1621 aatttattat cggtgaaatc atcggctgta atatcatccg gtgtaatccc gatgcgctgc
     1681 aataccatat tggtaacggc ttttactgtg aaaaaatcgg tttttattgc agaagtattc
     1741 caaccgccat taaataagtt cccggtcatc caaattgcta tggtgttcgt ttcgcaatat
     1801 cctttgcctg gaacagggtc tttatcagga ttgaaggtat agcggcgccc caactcataa
     1861 ataagaatat cgttttgctg aaaactaata tttcttgcaa tgctttcgag tccaccgaat
     1921 aaaagcgtcg gacgcatgca atttaactcg ttacttaacg cattatgtat ttcgaccaca
     1981 ggttttttat ccgggaaaag ctcaaaatat cgctttcttg tgaagctggt acacatgatt
     2041 tcggagaagc cccttccagt aagcatttca gcaatatttg cttcaatggc ggttctgtcc
     2101 ggtttttgtg taaacgtcag cgacgacacc atttttgaag gaagtgtaac attgttatag
     2161 ccatataccc ttaaaaagtc ctcaattata tcagcttcac gggtaacgtc aaccctatat
     2221 gtcggtacaa ttgctgaaaa tccgctttcg ttaacagaag taatttctat ttcaaggctc
     2281 ttcagaatac tgtgaataag ctcttttgta agctcaacac cacacaatct ttcaaagtgc
     2341 ttgtaaaggc aattcaccgt gaccggtgtt ataggttgtg ggtattgatc aataatatcg
     2401 gatgcgattt tacctcccgc aagttgaaca accaggtccg cagctctttt aagggcatat
     2461 ggtgccatat ttgggtcagc tccccgctca aaccggaacg aagagtctgt gctgattgca
     2521 tgacgctttg ctgttctgcg cacccatgaa gggttaaagt atgcactctc aataaaaaca
     2581 ttctttgttg tatcagaaat cccggcatcc attcccccga aaactcctgc gatgcacatg
     2641 ccttccgagc tgttgcatat catcaggtca ttgcacgaaa gcttcctttc aatgccatcg
     2701 agtgttacaa agttcgtatt ttcaggtaat gttttaatga taacttttcc tccgcttatt
     2761 ttttcagcat caaacatgtg taagggctgc cccgtttcaa acattacata attgctgata
     2821 tccactacgt tatttattgg ctttagccca atggctttca agcggttttg caaccattgc
     2881 ggcgatggtg aaatgctaac atttttaatg tatagaccca tgtatcgcgg acatgcttca
     2941 atgttctcga tatgaactgt aatttccgat ggggtagagg gataagggag ttgtggtacc
     3001 tcgggtaaac ggcatactac ttctcttgtt ttagcgaaga aggctgcaag atcgcgtgcc
     3061 acaccaaaat gagatgcgct gtcgatgcga ttgggtgtga tatctacttc cagaacataa
     3121 tcggtttcga tgccaaataa ggctgcagct ttcataccta ccggagtgtc ttctggcaga
     3181 accataatgc cggcatgtga tttgccgatg cctatttcat cttcagcaca aatcattcct
     3241 tccgaaagtt ctcccctgat ttttgatttt ttaatagtca atggctcagc cattccatac
     3301 aaagttgcac caactgttgc aaccacaact ttttgtcctt tggcaacatt gggagcccca
     3361 caaacgatat caagcagatt gctgctacca atattaactt tggtcagact taaatggtcg
     3421 gaatttgggt gaggtatgca cgaaacaacc tcgccaacaa aaaggtcttc caatccacct
     3481 tcgatgcttt gaaattttgt aaccgacgaa acttctaacc caagattggt aagtatttca
     3541 gccagttgct caggcgattc ttcaaattga atgtaatctt tcagccatga atacgatatc
     3601 tccatacagt aattgattaa gagttgcaaa agtaagaaaa atgccgatta cttgttctgt
     3661 tgttactgta tttcaccagc atctgtacaa aaataatcca attataaatg cgccgatttt
     3721 atttctaagc ttttcaaaat aaaagaactc cgtttttttt gggtaaggac atttttttat
     3781 atttttaacc cataaacaaa atttattatt atgatacgta ctcgcgaaga ttatctgagg
     3841 tcgttaaaag caatgcgacc gaacatttac aaaaatggag aacttattca ggatgttacg
     3901 gttcacccag ccactaaacg tacagttgaa agccactcac gtgctttcga tgcagccaac
     3961 gacgaaaaat atcgtgccct ttttaccact gtttcctatc tgaccggtaa gcccatcatg
     4021 cggttcaaca gcctgatgaa aagtctcgac gatattatga acaatgccaa actgaagcgc
     4081 gaaatgtatc atttcacagg ttcatgcagt ggtggtgttt gtgttggctg gaatgcccag
     4141 aatgtgatgt gggctgtaac cagtgatgtt gacacggaat ttggtaccaa ttaccaccaa
     4201 cggcttgaaa aatggatatt gcaagccgaa gagcgtggaa taacctgtgc cggtgcgctt
     4261 accgatgcta agggcaaccg tacactgaaa ccctcacagc aagaaaaccc cgatacaaac
     4321 gtaagaatcg ttgaaaaacg caacgatgga attgttgtta gaggtgcaaa ggttatgatt
     4381 tgcggcgcag ctgcttcgga agaaattttt gtgttgcccg gatcttctta caaagatacc
     4441 gatgccgatt atgcggtttc ttttgttgta ccccgtgata tagaaggtct tacacttgtt
     4501 gaaacccgcc gccctagcga tggcagggag tacgaagaag gttgggatgt tccggacact
     4561 ggtatttcgc aggcatattt aatttttgac aatgtttttg ttccaaacga acgggttttt
     4621 atggcagggg agtttaaata taccggcaag gtaatcgaat actttactgc taattaccgt
     4681 gcatgcattg gcgcatgcgt agcagggcag ggcgatgtaa tgatcggagc tgctgtttta
     4741 atggctaggg ctaatggcat ttcgaataag aaattcgatg ataagtttac agtgatggct
     4801 gccaacaacg aaactacctt tggtttgggt attggtgcta tggcattggg gggacctcac
     4861 ccatcaggac cctggtttgc cgagtcaaaa attgctcacc tgaataaaat atatgttgcc
     4921 actttacctt acgaaaccaa aaggttgtgt caggaaatag gagggggcat tgttgaaacc
     4981 ggttgtactc cttcatatgc cgattttaag aaccttatct acggaaatac actattgaat
     5041 cttgtgaaag caggaccctg ctcttccgaa agccgactgc gtgccacacg tttatcggag
     5101 tggctcacga ttggtgccgg agtgccgggg tgtatgcatg gtggtggatc acctgacggt
     5161 gcgcgcatgg tggttaaggc atttacccca tttgagaagt atgttgaaat ggcaaagaaa
     5221 attgccggta tcgaagaaga tattccggat cccgctgctg ttgcgaaata atcatcgcaa
     5281 cattacatgc tgtgagttta aatgaattga gtttataaca aataataagg aggaatcatg
     5341 tttcagtttt tcaatgaaga tcaaaagatg cttcaaaaag ttgttagaga ttttgttgaa
     5401 caggaaattg caccgtatgc agccgaatgg gatgataaag atacatgccc agttgaactg
     5461 tttaaaaaaa tgggtgaaat gggtatgttg ggagtttttg ttcctgcaca atatggtgga
     5521 gccggattgg gccttactga aaggaatatt attcttgaag aagtttcgag gcactccgca
     5581 ggctttggta ttgccatgat gacgcatgaa ctggcaattg ccgcaattgt aaactttggt
     5641 agcgaagaac aaaagttgac ctatttatct ccgttaattt caggcgaaaa aattggtgga
     5701 ctttcggtta ctgagcccgg aggaggatcc gattttggaa accagatggc tactatcgaa
     5761 aaaactgata ctgcctatgt aattaatggc agaaaatgct tcatcaccaa ttcgcataat
     5821 gccgaaatta acgtagttac cggaacttgt ggtgtaaatg aaaaaggacg taagctgatc
     5881 agtgccatta ttgttcctcc gggaactccg ggatggaatg ctggacggaa agagcataaa
     5941 ttgggacttc gcggctctgt gactggcgat attatctgcg acaacattgt tgtaccacat
     6001 aattgcatta ttggtaaaga tggtgacggg tcaaaaattg caatgcatac gattggtcat
     6061 ttcggaaggt caggaatgtc ggcaattgct gtcggagtgc tgcgtggttg cctggaagag
     6121 ggggttaaat ttgccaagga aagaataata tacggaaaac ccttatcaaa tttgcagtca
     6181 attcagttta ttattgccga aaataaggtg gattacgatg ccgctcatgc catgttgtat
     6241 aatgcaagcg caatttacga ccgcaaacag gaatgtgtat ccgacatagc tgccgtgaaa
     6301 ctgttttcgt cggaagctgc cgtaagggct tcaaagagaa ctatcgatct gatgggtggg
     6361 tatggaatta ttaacgaata tccggttggc aggttccttc gcgatgcaat ggcaattata
     6421 ccctccggtg gcacatccca tatcatgtcg gttattatag cttccggact tacccgatag
     6481 ggtctgtcat attcgtcatt ctggaattac ggtatgtata ataattttac gaaataatta
     6541 tgccgttaca aatagcagtt tgcataaaac cggttcctga ttcaaaatac tatgataaaa
     6601 ttagtattga ccctaaaacc aaaacctggg acaggtcagg tattccaatg gtaatcggtg
     6661 accttgataa aaatgcattg gaagcagcat tggttctacg tgagaagttt cagggtaaaa
     6721 tttcggtttt cagcatggct cctgaattta caagggaggt tatggtgcag gcattagcaa
     6781 tgggggccga tgaagctttc cttctcagcg accgctcttt tgccggtgcc gacacccttg
     6841 ccacatccta tacgctggct tcagcaatta aaaaggcagg gaacttcgac ctggttctta
     6901 ccggcaatga aagcgatgat ggcagtacag ggcaggtttc tcctcaactt ggcgaatggc
     6961 tgggttttcc acatctgatg aatgtggttc acctggattt cgacggcgaa ttattgtatg
     7021 ttgacacact tgccgaaacg ggcatttacc attatcaagt aaagctgccc gctgttattg
     7081 ctgtaacgcg gaaagttaac aaacctcgcc tgataggagt gatgggttta atgaaagcta
     7141 aggcaaaacc tttgaatttg tggacagctg ccgaccttgg tgcagacgcc aacagattgg
     7201 gtttaaaagg ctcacctacc caaccgggcg atatggtttc gcctgatact aaccgcaaag
     7261 ccgagagaat tgatggttct cccgaagata tagccgaccg tattattgtt gaattgcgaa
     7321 aagcaggaat catcggatag taatggaatc aagcgataac acatacagaa ttctggtatt
     7381 tgccgaaatc atcgacaata caatagcccg aaattccctc gaattgttag gggaagccaa
     7441 cagaatgaag aaatgcatta agaaaagtgt tgaagtaatt gcagcattta ttggtaacga
     7501 tgtacatgcc tttgctgata tggctataaa gtacggtgcc gatacgtgtg taatttttaa
     7561 caacactaat ctggaacatt atcggagcga ctgttacgga gatgcacttg caaaaattgt
     7621 aaaaaagctt caacctgaaa ttgtactaat cggtgctaca ttttttgggt cagaaatagc
     7681 acccacagta gctgctaaat tgcaaaccgg tttggctgct cattgtactg aacttaaaat
     7741 cgataataat gaaaggttta ttcaggtagt acctgctttt ggagggaaag tcctcggtga
     7801 aattataacc cctgtgcaca ggccgcagat ggcttctgtt aaacccggaa tcatgacttc
     7861 gttatatgat gaaaagagaa ccggacaaat tgcctacttc tctgaagaat tatctgattt
     7921 tggtgataga ataaaatttg ctggaattac cccggttaaa gcatcgggta agcctttgca
     7981 cgaagcagat tgcataattg gggggggcta tggtattggt agtcgggaaa actgggaagt
     8041 tcttacgaaa attgcagctt tactgaatgg cgcaaccggc tgtactcgtc cggctctcga
     8101 tgagggatgg acaaccggtg agcagactat gataggtacc agtggcgtgg ctgtcagacc
     8161 caaagtgtat atcaatgctg gaatttcggg tgccgcgcat catacttgtg gcataaaaga
     8221 agccggtttg gtaattagca ttaataagga cgaaaatgcc cctattttca acagttccga
     8281 tattaagatt gttgctgact ggaaaaaagt tcttcctgta ttgttggaaa aattgaatca
     8341 gttgaacgat gcaggttcat tgaaataaaa aaagccgcat tgagcggcaa ttttggtaac
     8401 aacacagcaa ttaaaccaat ttattgtaaa aacctggtat tgattttttc ccaatcgatg
     8461 atttcccaga agtttctaat gtagtcaggc cttaaattct ggtagtcgag gtagtaggca
     8521 tgttcccaca catcgcaagt gagcaatggt ttgtagccat cacgcagagg tgtgtttgca
     8581 ttaccggtag ctaatatttc aagtttaccc tgttggttta ccacaagcca tgcccagccc
     8641 gaaccaaact gattggctgc agcatttgca aattgctcct taaatgaatc aaatgaacca
     8701 aactgtgtgt cgattgcctt tgcgagttgg cctacgggta cacctccttt gtttttacca
     8761 aaaccttccc agtagaaagt gtgattccaa acctgtgccc cattattgaa aataccgcct
     8821 tccgattttt ttactatttc ttcaaggctt ttaccttcaa aattggttcc tgcaatcaat
     8881 gcattcactt tatttacata agcctgatgg tgttttgtgt aatgaaattt tactgtttgc
     8941 tccgatatat acggttccag agcattcatc gcgtaaggta attcgggtaa tgtaatcata
     9001 gttaaaatat ttttaagtaa ttattttttg taattatata tttataacaa taaacctatg
     9061 catttgttca attcttcaat aaaaaatacc ttgaatcagt ttgcagaacg aaaagaagta
     9121 ctactactgt ataattcttt tgataactct caaattgtgg gtatatatat gcctgtcggt
     9181 attaaaaatt ccgttatgat caatattatc gatgcgaacc ttgcccgacg catgaactat
     9241 ttttccattt tctgcaataa tcccaacatg cttaatgtgt ccattattat catcgaagaa
     9301 tgcaagatca cctggtttcg cctcggaaag caggtttacg gtagttcctg cctgaacttg
     9361 tttcgaagca tctcgtggaa tgcttatgcc aagaattctg tacacagttt gggtgaggcc
     9421 agaacaatca attccccatg gagtgcgccc gccccataag taaggactgt tcaaatactg
     9481 cattgccagt acaacaggca actggttgtc ggtagcggta aattttacag gtgtcttgcc
     9541 gatgatcgta aaattgttac ccgaaacctc aaattgattc ccgttgtgat agggtaaagt
     9601 agtacctccg ggtaaataaa acctttcgtg tttttgattt tctatttcta aaaagggttc
     9661 cgaaaccacc gaaacttttt tgtcgctaca cagttgctca tatttttcgt gggtaatgaa
     9721 actaatggtt tttgtatcga tccatccttc gtagccgtca tgaaaaagtt taacttttgt
     9781 ccagttactc ctttcaatta acacctcgaa ccattctcca aagagcagct ggcttaacat
     9841 ttcgctacct tccgaaggtg aggaacgcag tggaacaatt gctatttcgg taataccata
     9901 tttcatatat ttcaacttct taattgtact cttcccgtta cttacgcttt aataattaat
     9961 cagatctcct tttttaatgt tcaggctgtc gcatgtgccg gcattaagtt ctatcacata
    10021 taaagccggc ttatcggagg caaacgattc atcgcttaac acaggggtgt ttttatgaat
    10081 ggtaactatt tccttatgtg catttacaaa aatcatgtca agtggtatta gggtgtttcg
    10141 catccagaac gactgtactt cttcattatc aaaaatgaac agcatgccct gattaggttt
    10201 catttctttt cggtacataa gacccatggc acgcttttcg aaagttgatg ccaattctat
    10261 atcgaaagtt gcaatgtcgc gaagacttcc tttatcaata aaaacaagct ccccgtcttt
    10321 aacaaatttc ggagggttat cgaagttgta aaccacttgt ttcagttctt tgttgttgct
    10381 cacatcaggg cgtttgggct taggattgca cgcaaatata accattagac ctgtcagaat
    10441 aaatactgta tattttaaaa tgggtttcat tgctacgaca tttgcagtgt ataaagttta
    10501 gtatataatc cgtttttatc aattaaatcg ttgtgcttac cgctttcaac aatttcacca
    10561 tcatgaataa caaatatttc atcggctttt tttaccgtac tcagccgatg ggcaattaca
    10621 atagaagttc tgtttttcat caggttctca agtgcttcct gaacgagttt ttctgattcg
    10681 gtgtcaagag ctgaggttgc ttcatcgaga atgagtatcg gtgggttttt taaaacagcc
    10741 cgggcaatgc tgaggcgttg tttttgccca cccgataact tgcaaccgcc atcaccaatg
    10801 ttggaattat aaccatgttg agtttcaatg ataaagtcat gcgcgttcgc aacttttgcc
    10861 gctgcaacaa catcttccat tgaggtgttt tcaataccga atgctatatt gttgtagaaa
    10921 ctgtcgttaa acaatattgg tgtttgattg acatatccta ttaattttcg taaatctttg
    10981 atttttaaat ctttgatttc attgccatca atgcttatga tcccttgacg gatatcataa
    11041 aaccttggta gtaggtcaac cagagtcgat ttacccgacc cgctttgccc aaccagggca
    11101 atggttttac ctttttcaat agtgatgttg atgtttttaa gtacttcgtg ggtttcgtta
    11161 tacctgaacc ataccgaagt gtaattgatt tggttattga atgaattgat tgaaacggcg
    11221 tttggttttt ccgtaattct ctcttcactg ttcattattt catttaccct atcaagcgaa
    11281 gccagtccct ttttaatatt ataccatgcg gttgaaaatg cttttgcagg tgtaatgatc
    11341 tgactgaaaa taatcagata tccgataaat gcctgcgatg aaagcccgga tgtgttttgc
    11401 agaaccattt tacctccata ccacataaca aatactattg caactgttgc aagaaactcg
    11461 ctcagcggac tagcaagaaa acgccttctg ttgattttat tcatcaggtt tgtatattcc
    11521 tgattgccat gaataaattt gcgacgaata tggttttcag cattaaatgc tttaataatt
    11581 cttaaaccgg ttatcatttc ctcaatttgc gaaagcatct gccccatttg ttgctgaccg
    11641 atcatcgagg tcttgcgann nnnnnnnntt tccaacaata aagccaacta ccggcagtag
    11701 tatcattaca aacaatgtaa gctgccagct cataagcacc agtgttataa gaaagaacag
    11761 tattttaagt gggtctctga acaacatttc aagagaactc ataatgcccc attcaatttc
    11821 ggtaacatcg ttcgacattc tggaaatgat atttcctttt ctctcttcgg aaaaataacc
    11881 cagcggaagc cctgatactt tgttgaaaag ttggtttctg atgtcgcgga caacattgtt
    11941 gcgtataggt accatgaaat ggttggccaa ataccatgat atagttttaa ggaaagtaaa
    12001 aaccataaca agcataccaa ttgcaaacaa tgcattggcc ggtccgcttt ctgtaataat
    12061 tttactgagc cagaagtaga agttgttttc gatgctcgca gcactcatca gatcgagagg
    12121 aacgcgctct gttaccatgg gctgagtacc gaaaagaatg ccgagaaatg gtattaccat
    12181 cccaaacgaa aacaggctga aaattgttga cagagcgttg aacagtacat taagtactgc
    12241 gtaccacttg taaggaacaa tgaatttaaa aattttagca atatctttca tcaatctgca
    12301 ctttccgttt tttatttccc tatataatta taaggtgtaa tctgaagcaa ttcattttta
    12361 atattttcat ccacattcag tgctttgata aagcttgcaa tatcttcttt cgaaaccaca
    12421 ttgttcttgc gagtcaatgc tttcagggtt tcgtagggct cgggataatt ctcccgtcgc
    12481 agaatgcttt gaattgcttc agcaaccaca gcccaattcg aggcaaggtc ggcactgatt
    12541 ttatcttcgt tcaacaataa cttacccagc cctttttgta acgaattcaa ggcaataata
    12601 gtatgtgctg aaggaacccc cagattgcgc aaaacagtgc tgtcggtaag gtcgcgttgc
    12661 aatcggctta ccggaagctt ttcggcaagg tgggtaaaaa tggtgtttgc catccccaga
    12721 ttcccttctg cgttttcaaa atcaattggg ttcactttgt gtggcatggc actggagcca
    12781 acttccgaag ttttgatttt ttgtctgaaa tattccatcg aaatatacgt ccacatatcg
    12841 cgggcaaaat cgatgagaat agtattaatt cttccaagtg tattgcatat agcagcaaga
    12901 ttgtcgtagt gctctatctg agttgtaggg taacttctcg ataaatatag tttcttcgtt
    12961 acaaatcttt cggcaaagca atgccaatca atatttggat aacttacgta atgagcgttg
    13021 agatttcccg ttgctcctcc gaattttgca gagaaaggga ttgctttaag ttgttgaaac
    13081 tgagtttcga ggcgtgctga aaaaacgcga aactctttcc cgagacgcgt tgggcttgca
    13141 ggctgaccgt gtgtatgcgc cagcattgga acatcattcc actgctccga aagatgattg
    13201 atgttatcaa ttaacatttg taatgcaggg gcgtattgct cttgtataaa atcacgtagt
    13261 tgtgcaggaa tagctgtatt attaatgtcc tgagaagtta gtccgaagtg caccaattca
    13321 cgatatttac cccagccttg ttggtcgaat ttttctttta ataaatattc aatcgctttt
    13381 acgtcatgat tggttactga ttcaatttct ttaacccgta ttgcatcact ttccgaaaaa
    13441 cctgcaaccc aattccgcat gatttgaaac ccagcatttg ggaaatcagc aagcaccggc
    13501 acgggtattt cgcatagcgc aataaagtat tccacttctg tgtgcagcct gtaacgaata
    13561 agtgaatatt ccgaaaaata tgccgacaaa ctttcaactt gtttgcggta tcttccatcg
    13621 atgggtgata cggcgtatag actgaatgta ttcatcaaaa caaatgtttt gtcaaaggta
    13681 aaaatttata actgatttta aatattttcg gtaagcgata ttgttttata catttgcatt
    13741 aacatagact tgcgatgaaa atagctgtag tatcatacct caataccata cccttcaggt
    13801 atgcaataga gaaatcccct gttaaaccgg ttggggttga atacatatat tgtcctcctt
    13861 cggagtgcgc atctttgttg ataaataata aagctcaggg tggcctgata cccgttgcag
    13921 caatcccatc actgaaaaag cattatgttt tttccgatta ttgcattagt gcttaccgtg
    13981 aggcttattc tgtagttttg ctgagccaaa tgcctttatc cgaaattaaa acggtgattc
    14041 tcgattatca aagccgctca tcagtagcaa tgttcaagct gctgaaccaa cattatttgc
    14101 acttcaactt tgattttgta cagggccaag aaaacttcga gaagcagatt tgtgggacta
    14161 cagccggtct ggtaattggc gacagagcaa tgaagtatcg gatgcatgct gaatatgttt
    14221 acgatttggc agagatgtgg tttgaacata ccggcttgcc tgcagttttt gctttgtggg
    14281 tttccacagt gcctgttgca tcacaaatta aactagcatt cgattcaatg atgcaatttg
    14341 gcatgaagtc ggttgatgag gctattcact acttcaaccc tgcttacgac agggaactga
    14401 tgcacagtta ccttaccgag aatttatgct attccctcga ttccaatagg cgaaatagtg
    14461 ttaatctatt tctcagcctg ttgaataacg gcgattttaa aactaatttt tcatttcaat
    14521 gaacaaattt atgtactcag tcagaaacta taaaagcgat gattatcagg atgttgaaaa
    14581 tatatggaat gcttgcgata tgggtggttc agtgcggggc gacactgcta ctattgttga
    14641 aagaacttta gccggtgggg gagcattatt gttgctggta gcatcggatg gcgaaaatga
    14701 aacaataata ggtacaacat ggattaccaa cgatgcacga aggctttatt tgcaccattg
    14761 cggcatacta cccgaattca gggggcttgg gctctcaaag cttctggttc aggaagcatt
    14821 gctgatggca aaacgactgg gtatgcaaat caaactcgaa gtacaccgaa acaataccgt
    14881 tgctaaagca ctatatgcga aaaaatactt cacctacctg ggtgattacg aggttcatat
    14941 tgttagggat gtaagtaaaa tacccgacta attgaagtca ctgcacaacg atattctacg
    15001 actggccgtt ccaaacattc tcaccaatct tacagttcct ctacttggtt tggtggatat
    15061 cggtttaatg gggcacctta ccgacgcagt atatcttggt gccgttgcct tgggatcgac
    15121 catattcagt ttgttatatt ccggattgtt gtttctaaga atgagtataa ccggattcac
    15181 agcgcaggct tatggagccg gatatcacag agaaagctcg ttggtattag gcaggggtgt
    15241 ttttcttgcg ttaattatgg gcgtaatgct gatattagct cagcacccaa tagaatgggg
    15301 tttattcaaa ctggttaatg ccagcgatgc agccgaaagg attgctgttg attacttcag
    15361 agtacgaatt tgggctgctc ctgcaacttt atgtgttttc gtttttaccg gctggtttct
    15421 aggcatgcag aattcacgca tacctttgct gatagcagtt gtaataaatg tcgtaaatat
    15481 attgctaagt attctatttg tcaaccgatt cggaatgaat gctgccggag ttgcgctcgg
    15541 aactgtgatt gcacagtatt gtggacttct gatctccgtg ctttgctttt taaaatacta
    15601 tggccgcttt cggttgttgt tcagcttatc gggtatgatt cgtatggaca ggcttacacg
    15661 gttttttaag gtaaatggtg atattctggt aaggtcaata ctgattactg gcactttctt
    15721 ttacttcaat gctgtttctg cttcaatggg aaatctggtt ttgtcggtaa attcaatttt
    15781 actgcagttt ctttacattt tctcttattt tattgatggt tttgcttttg ccgcagagtc
    15841 gctcaccgga aaaatggttg gatcgggaga caattcaggg ctgcgaaaac tggtgaaaac
    15901 attgttttta tggagcggaa tattaagcct tgccttttcg ctgtcttacc ttgtttttgg
    15961 aaatgatttt atatcgttgc tgacagataa cagcgaaatt gtttctcagg ttaagccatt
    16021 tttcttctgg gcagtgttaa ttccagttgt gagttttgct gcatttgtct gggatggtat
    16081 ttatattggt gcaacagctt cacgctattt acgcaatgcc atgctgattg ttgttttggt
    16141 attttttcta ccggcaagtt atttcatgaa ggatgcctgg ggaaatcatg gattgtggtt
    16201 gtccctgatt atttttctat cggccagggg agtgatgtta ggcttgt
//
LOCUS       DAOOQP010000070        15485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4659_length_15485_cov_4.730784, whole genome shotgun sequence.
ACCESSION   DAOOQP010000070 DAOOQP010000000
VERSION     DAOOQP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4659_length_15485_cov_4.730784"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PLK75_08195"
     CDS             <1..1371
                     /locus_tag="PLK75_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOY39265.1"
                     /translation="EIIIFILMIGGAFWIMNASKALDVGIMAFIRTTGRLEKYRFLRK
                     LGVNNIVMVLIMLMFSLFGAIFGMSEETIAFVIILVPLAISMGYDSITGVCLVYVSAH
                     LGFAGAMLNPFTIGIAQGLSDIPIFSGIEYRIICWLLINAVGFSFILVYANRIKRKPE
                     RSLTHKIDEFWRNRKIEGEQHISYYTPKIAWVVYAILVLVLAAFAWFYPSTSLHIGNN
                     NITGPVLPVICGLFALTAFFSLRKSVHYFNLNLLAYTIVFLIVGVMGYQWYVMEIGTL
                     FLAMGLSAGISINMSANNLVKQFLEGAKDIFSAALVVGLAGGIIVILTDGYIVDSIMY
                     GLSKTMQGFGKMGSLSTMYGIQTALNIIIPSGSAKAALTMPIMAPFSDLIGVSRQATV
                     MAFQFGDGFTNMITPTSGVLIGALGVAKIPYDIWFRWVWKLILALFILGIIMLLPTIY
                     MQLNGF"
     gene            complement(1432..3099)
                     /locus_tag="PLK75_08200"
     CDS             complement(1432..3099)
                     /locus_tag="PLK75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOY39266.1"
                     /translation="MKKLTAFIIPGVFVAMLGLSIAFTSCDPETTEDVITALGWLFEN
                     EDTTTIPDDINLATAYGDESLPASVDLSMYFPPIGNQGQYGTCVAWACGYNLRSYLNA
                     RANDLTTDDMIYSANVFSPKDLFWAIPNSEKGADCNGTQFESALDVMVSRGIARMSVV
                     PYSAMGNCSSSPSSDWTSDAGNYKILNYRQIDQTVDNIKRYLYQGKAVVFGAKLGDEF
                     MNASGDVVLTSQTYGYTGQHAYHALILCGYDDNKGPNGAFRVVNSWGTGWGDDGYIWV
                     DYNYFVQQDYFAFASFVASNIEDEPDLDNPTSGYDLQAWELNDIDDSEQTDATYREAL
                     YNVYNTGENSLNASSDWNILYMYYNAYNANDYGIILYDMYTDNYGSYGFFDRPDPVPS
                     DVPAADYWATYLDFPGQTSIVNEVARQLYGNTAERFSWKYQMPQINGYYYLILIADGF
                     DAFSEFNEDNNYFYYAYENGDPLYIVNGVIQGPPAAKMAVKNSKTPVKGASSDFQSVR
                     VGKNVNAYSTGEIRKLIDDRRQSGAMARKVMQWQNSDAAKKVSKSIK"
     gene            complement(3124..4107)
                     /locus_tag="PLK75_08205"
     CDS             complement(3124..4107)
                     /locus_tag="PLK75_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOY39267.1"
                     /translation="MKHGILLFALMSVVLVSCGNLSVQNDSKGKEIDADIVIKMINSG
                     EPVYLEDKIITGDLDLTKLKNRSHENKASIRAYIDEPLSFINCTFNGKLIAAGFDAEK
                     KYIKTSFAKSVVFTGCVFNEEVILKESVFRGLTDFSKCIFSKKADFSGSYFDCRDVYF
                     SETSFNGESNFNDSKFTGNVSYFKSFFKETSLFQQITFMGTVNVASATFSAYTDFSAS
                     SFYGLLNANYCKSGKKLLLNYCRFFGKADFIQAEFSGNFEIKEAVFYFQARFKESIFS
                     GKAEFVKCLFIDSAPDFTGVKKNENTEIVFTGNMQSSHNELDTIEISDVKE"
     gene            complement(4219..6165)
                     /locus_tag="PLK75_08210"
     CDS             complement(4219..6165)
                     /locus_tag="PLK75_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY39268.1"
                     /translation="MTKTITVFLFLSLFCSCVSLSQPKQSYHTQSKKAIKNFETALEL
                     ANNRKNGDALQLLYKAIEIDSTFIEAYVLMNEIFTAQAQYKESTEMLKKIIQIDPYYD
                     LNYYMVLAQYEYKTGRYDESKMHISFFMENADLTEEEKSKTEYLLKRVEFAIVAYDNP
                     SAFEPINLGNGVNTKYDEYWPSLTADEEILVFTRLIPGDEYGNASGEYQEDVFISHLK
                     DGNYMPAYKMPGETNTEYNEGAQCIAQNGKICILTACNMPDGYGSCDLYISYITPQGW
                     SKPRNLGKMVNLGSWDSNPSLSADASTLYFASNRGGGMGKMDIWSVALGPDGFPVSNA
                     VNLGPVINTEFNECSPFIHPDDRTLYFASDGHIGLGSYDLFLSRKDPVKGWGNPENLG
                     YPINTQNEERSLIVNAKGDVAMFASARERGKGLDIYKFNLPPNAKPVTVSYVKGYVYD
                     VETGNRLKADCKLIDLETEILVAEETSDAQTGNYLVCLPIDRDYAFNVSKQGYLFFSE
                     NFSLSNLEDPSKPYIMNIPLQPVKEGTTVVLKNIFFDFNKFDLKETSFAELNVVIKFL
                     IDNPHFRIEIGGHTDNVGSKDFNRSLSENRAKAVYDYLVSKGIAKSRLSYKGYDYSMP
                     IATNDSEEGRALNRRTEFKIIGMK"
     gene            complement(6162..6779)
                     /locus_tag="PLK75_08215"
     CDS             complement(6162..6779)
                     /locus_tag="PLK75_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOY39269.1"
                     /translation="MLKSKSIDSIPVIEEFYSVQGEGFHTGKAAYFLRVGGCDLGCRW
                     CDSKESWDIDVHSYIPVSEIIERILQTAARTVVVTGGEPMLYDFTGFTRIAAQHGIAC
                     YLETSGAYPVSGKWHWICLSPKKQEPPVPANFTMADELKIVIYEESDFNWAEHCASQV
                     KHDCHLFLQPEWSRLRKNANTIAEYVKQNPQWRISLQSHKYLNIP"
     gene            complement(6786..8003)
                     /locus_tag="PLK75_08220"
     CDS             complement(6786..8003)
                     /locus_tag="PLK75_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39270.1"
                     /translation="MKRLFITSGLLLSACILFGQSHDKAVYSDFKAGFYDNVILKDIN
                     PDFVRTSFPKRFTMDFSGREFPTDIKKYTRSWHSAPKSQGNTGTCWCFAVTSFIESEA
                     YRLHGLQPDLSEMYTVYWEYVDRAKDYVNTKGETYFAQGSEANAIKRLWPVYGIVPET
                     AYSGLTGNHKFHSHDAMVDEMNEYLEKMKESGNWDLATAEKDIRGILDKYMGKPPVEF
                     IVEGKKYTPQTYLSEYLFLKPTDYFSFMSTMAFPYNEKHELVEDDNWWHSEDYYNVTP
                     DDFMKIINDAITNDFTVCLCGDVTEPGYDSYAEVAVVPDFDVPSELINEKSRQLRLEN
                     GSTTDDHCIHIVGYQKVGDDYWYLIKDSGSGGFDGPNKGYRFYHEDYVKLKMMNIMVY
                     KNAARNILDKIIK"
     gene            complement(8117..9151)
                     /locus_tag="PLK75_08225"
     CDS             complement(8117..9151)
                     /locus_tag="PLK75_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39271.1"
                     /translation="MTFDHDGTCSLCSKQKTFQPIGENKLLELLENAKAKKAPYDVLV
                     PLSGGKDSTYILHLAVNVYKLRVLTMTFDNGLFSDIARANINLALQATGVDHVWCRPD
                     EKLLNKIYGYMLLNTGDICGTCDIATKANIIKVACDYRIPYVLYGTSPLEEDSFVPDS
                     IQDVARFKYIIRKSKEFSNSEINKFLIYPNLNNFVLSVRKSTGAFAREIRPLFYLNPV
                     TDREIGCIISQTMGWRDDSREYSKHLDCIAEPLTNYIRNRIYGYERRLCQFSNMVRNG
                     EISREHALQLYQEDCIETIPQNAAEVLAQFGLRTYDIEKIVSYKPLQYEKNTSGWNSL
                     YARIMKIRKK"
     gene            complement(9221..10474)
                     /gene="hutI"
                     /locus_tag="PLK75_08230"
     CDS             complement(9221..10474)
                     /gene="hutI"
                     /locus_tag="PLK75_08230"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOY39272.1"
                     /translation="MKTIIKNIRYLVQAERKCSMLVKGAEMANVPVLENAWLLFEDDL
                     IKDFGRMEAYPFTSIITDASTTEIDASGKTVMPSFCDSHTHIVYAGSREIEYSDKIKG
                     LSYEEIAKRGGGILNSAALLHKTSEDELYEQSIVRVFEIISQGTGAVEIKSGYGLNTD
                     DELKMLRVIKRIKDTTPLVVKSTFLGAHSFPAEYKNNRDEYVDLIINEMIPVVAAEEL
                     ADYIDVFCDTGFFTTEQTERILMAGIKYGMRPKIHANELAKSGGIQVGVKYSALSVDH
                     IEYTGKEEIAALLSGTTMPTILPGAAFFLNMPFAPAREMIKAGLPVAIASDYNPGSSP
                     SGNMKLMMSFACVNYKMLPQEALNAATINSAYAMGVSDVAGSICPGKLANIIITKPIP
                     SLEFIPYAYGSDLIDTAILKGKIIN"
     gene            complement(10513..11793)
                     /locus_tag="PLK75_08235"
     CDS             complement(10513..11793)
                     /locus_tag="PLK75_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39273.1"
                     /translation="MKYLAFFLLTCWYLALFSCRSQDNRRQSLTINDKGIMALIHEKC
                     PDADIVEIEESKEYTEVNYFCGNTLYETGIKNGQIIFTESPADISVVPIDKINKKLEK
                     KYQGWILDEISLVELPDTSFLKVEIIRDGIEQNVYFTTSGKFYKSKSIAVSDKWNSSK
                     LSEIALYKNSPYDFFNTDSVFEMPDVLNELSGIAKLTNESVICIQDELGVVFEFNLIS
                     ESITKMHRFTDVGDFEDIAIAGNNVFVLRSDGTLFSFDYYHNNSAVNQSILPINSLNL
                     EGLYYDKTQNCFYVASKEHLLGGDETKRLVFKYLPGQASEIESAVVIDQLELNRLFAS
                     QFGSIYSGKVSFNPSAIAVHPVTKELYILSSIDKMLVVCKDGKILRFYPLPADIYFKP
                     EGIAFFENGDLLIANEGDKKGLVKGNIVFLPYRN"
     gene            11951..12757
                     /locus_tag="PLK75_08240"
     CDS             11951..12757
                     /locus_tag="PLK75_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39274.1"
                     /translation="MKTLVFALSLLIIASIATAQENTSPVSDKPADDSVFITQHLLGL
                     NVFPAFGILGGGITNNSKITLQYKILTKKFNYRFSLNYLNFYRSHNAFDIVGIVADTT
                     LPASIGDDTIFQSLKIRNYFNQVNTYDLRFGAEYAIQRDDIRFYIGLGGIIGIHNIVN
                     SYNYFYKPCPEEGYPVDNLYFGYEYLNELDNHSRATSFLKMGADLSLGVDFNLADNCV
                     VALQYTPEFAYYRWLSDKKTNDTDDVFPNRLNSFWVFSPDFIDLLVYIRF"
     gene            complement(12846..15131)
                     /locus_tag="PLK75_08245"
     CDS             complement(12846..15131)
                     /locus_tag="PLK75_08245"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOY39275.1"
                     /translation="MRHFNKFFFVLLIAAMLYEPMSLRAQAFTEGFNDITTLTGSGWF
                     MQNNSVPLGSTNWFQGNSDVFSAQSGAATAYIGTNFNNTTGANTISNWLVTPSVTISN
                     GDVLSFYTRTTDDNTYPDRLQVRMSTNGASTNVGTGSAAVGDFTTLLLDINPTLVTSV
                     YPYLSWGFYSITITGLSAPVSGRFAFRYFVTNGGPSGTNSDYIGIDEVIYTPFVCPAV
                     TLSPLGSVPDGTTGTVYSALITQTGALGPASFEVTDGNLPPGISLSTSGVLSGLPTLA
                     GTYNFTVTVASDYNCSKSEDYIFQVNCPANPIVFPEFEPICSNIGNILLNTATPSGGT
                     YSGTGVENNFLDPTSGSQTITYTYTDMYGCEYSDENDIVVNIAPEVTHSAIDPICIDN
                     GMVSLMGGSPENGFYEGNGIEENNFNPEAGSQMLHYIFIDDNQCSDTAYIQVTVYELT
                     TLVVMDDMEICQYEPITLSASTNGTAAWYISGSEDELTELTVTPMETTEYVVVAQNGV
                     CAAVSDTVMVTVKNVVETPAIINGNNVVCQGQGDVYYSVDPQTDALSYIWTLPNGAYG
                     ASTTNEIYISYGIWAVSGNITVAAVNDCGTSDAVILEITVNQKPPTPIIIQSGATLIS
                     NSGTGNQWYNDDGAIDGANSQFFDYEGDGNYYVIVTVNGCASDPSNVISIINSSVFNG
                     LSGSIIGVYPNPARDQITVSAAGNLQFVVAEIVNSLGQVIEQAHFSGSFIFDLTGYPE
                     GLYFINATINGKNIATRFIKE"
     gene            complement(15219..>15485)
                     /locus_tag="PLK75_08250"
     CDS             complement(15219..>15485)
                     /locus_tag="PLK75_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39276.1"
                     /translation="HFSRFFPAYKMKDVPPTPVETIEQACDIARKAGIKYVYMGNVQN
                     QAEFTSCPKCGFEIIDRKAYNIQIAPSFIGKCPKCGTGISGVWK"
BASE COUNT         4381 a         3349 c         3069 g         4686 t
ORIGIN      
        1 gaaattatta ttttcatatt gatgattggc ggcgcctttt ggataatgaa tgcaagcaaa
       61 gcattggatg tgggcatcat ggcttttatc agaaccaccg gtcgtctcga gaaataccgg
      121 tttttgcgta agcttggagt gaacaacatt gtcatggttc tcatcatgct tatgtttagc
      181 ctcttcgggg cgatttttgg catgagcgaa gaaaccatag cctttgttat tattttagta
      241 ccgctggcca tttcgatggg atatgattct attaccggag tgtgtcttgt atatgtctcg
      301 gctcatctgg gatttgcagg cgccatgctc aatccgttta ccattggtat cgcacagggc
      361 ttatcggata ttcccatatt tagcggcatt gaataccgca ttatctgctg gttactgatt
      421 aatgctgttg gattcagctt tatactggtt tacgcgaacc gcattaaacg caaacccgaa
      481 cgcagcctga cccacaaaat cgatgagttt tggcgcaacc gtaaaattga aggagaacaa
      541 cacatcagct attatacccc gaaaattgcc tgggtggtct atgcgatact ggtactcgtg
      601 ttggccgcat ttgcatggtt ctaccccagc acttcgctac atatcgggaa caacaatata
      661 acaggtccgg tacttccggt aatttgcgga ttatttgcct taacagcttt cttttcactc
      721 cgtaaatcag tgcactactt taacctcaac cttctggcct acaccattgt ttttttaatt
      781 gtcggggtta tgggatacca atggtatgta atggaaatcg gcacactatt tcttgctatg
      841 gggctaagtg ccggtatctc catcaacatg tcggcaaaca atctggtaaa acagtttctc
      901 gaaggggcaa aagatatttt ttcagccgca ctggtagtag gtcttgccgg gggcatcatt
      961 gtaattctta ccgatggtta tattgtggat tcaattatgt acggactttc gaaaacaatg
     1021 cagggatttg gtaaaatggg ctcactaagt acgatgtatg gaatacagac agccctcaac
     1081 ataataattc catcgggttc tgccaaagcg gcacttacaa tgcccatcat ggcacctttc
     1141 agcgacctga ttggtgtatc gcgacaagcc actgtgatgg catttcagtt tggtgatggt
     1201 tttacgaata tgattacacc aacatcggga gttctaatag gtgctctggg agttgcaaaa
     1261 attccttatg atatttggtt tcgctgggta tggaaactca ttctggctct gtttatactt
     1321 ggcataataa tgctactgcc aacaatttat atgcagttga acggctttta gtttactctc
     1381 aataaagcaa taaaaaagcc gctcttaaaa agcggcttca aataaaaacg tttattttat
     1441 tgatttacta actttcttgg cagcgtcgct attttgccac tgcattactt tacgggccat
     1501 ggctcctgat tgcctccggt catcgataag tttacggatt tcaccggtag aataggcatt
     1561 cacgttttta ccaacacgaa ccgattgaaa atcggagctg gcacctttca caggagtttt
     1621 actattttta acggccattt tcgctgcagg aggaccctga attactccat tcacaatata
     1681 taatggatcg ccgttttcgt aagcatagta aaagtaatta ttatcctcat tgaattcgct
     1741 gaaagcatca aacccatcag caattaatat caggtagtaa tacccgttga tctgcggcat
     1801 ctgatatttc catgaaaagc gctctgctgt gtttccatat aattgccttg caacttcatt
     1861 cacaatactt gtctgccccg ggaaatcgag gtatgttgcc cagtaatcgg ctgccggcac
     1921 atcgctgggt accgggtcgg gacgatcgaa aaagccatag ctgccatagt tatcggtgta
     1981 catatcgtag aggattatgc cataatcgtt tgcattatag gcattgtagt acatatacaa
     2041 tatgttccag tcgcttgaag cattgagtga attttcacca gtattgtaca cgttatacag
     2101 tgcttcacga taagttgcat cggtttgttc tgaatcatcg atatcgttca gttcccaggc
     2161 ttgtaaatcg tagcctgaag ttgggttgtc gaggtcgggt tcatcttcaa tatttgaagc
     2221 aacaaacgaa gcaaaggcaa agtaatcctg ctgcacaaaa tagttgtaat cgacccagat
     2281 atatccatca tcgccccaac cggttcccca tgaattcaca accctgaacg caccgtttgg
     2341 gcctttgtta tcatcatagc cgcataaaat cagggcatgg taggcatgtt gacctgtata
     2401 accatatgtc tgacttgtta gcactacatc accgctggca ttcataaact catctcccag
     2461 ctttgcacca aatacaacag ctttaccttg atacagatat cgttttatat tatccacagt
     2521 ttgatctatc tgcctgtaat ttagtatttt ataatttcct gcatcgcttg tccaatcgct
     2581 tgatggcgag gacgaacaat tacccattgc agaataagga actacactca ttcgcgcaat
     2641 gcctctcgac accattacat ctagagccga ctcgaattga gttccattac aatcggcacc
     2701 tttttcggaa tttggaattg cccagaacag gtctttagga ctaaaaacat ttgctgaata
     2761 aatcatatcg tctgtggtaa gatcatttgc ccttgcatta aggtagctgc gcaaattata
     2821 cccgcatgcc catgcaacac aggtaccgta ttgcccctga tttccaatcg gcgggaaata
     2881 cattgacaga tcgacacttg ccggcagcga ttcgtcgcca taggctgttg cgaggttaat
     2941 atcatcgggt attgtggttg tatcttcgtt ttcgaacaac caacccagtg cggtgattac
     3001 atcctcagtg gtttcggggt cgcaggaggt gaaagcaata cttaaaccca gcattgctac
     3061 aaaaactcca ggaatgatga aagctgtcag ttttttcatt ttgatatttt ttagtgttat
     3121 attttattct ttgacatcac taatttcaat tgtatctaac tcattatgcg acgactgcat
     3181 attcccggta aaaacgattt cggtgttttc gtttttcttt accccagtaa aatcgggtgc
     3241 tgaatcaata aaaagacatt ttacaaactc cgctttcccc gaaaatatcg attctttgaa
     3301 acgcgcctga aagtaaaata ctgcttcctt aatttcgaag ttgccagaaa attcggcttg
     3361 aataaaatcg gctttgccaa agaaacgaca ataatttaaa agcagttttt taccggattt
     3421 acaataatta gcatttaaca agccataaaa cgaagaggca gagaaatcgg tgtaggcact
     3481 aaaagttgca gatgcaacat tcaccgtgcc cataaaggta atttgctgaa aaagagaggt
     3541 ttctttaaaa aatgatttaa aataacttac gttgccggta aacttggaat cattaaaatt
     3601 tgattctccg ttaaaactgg tttctgaaaa gtacacatct ctgcaatcga aataacttcc
     3661 cgagaaatcg gcttttttcg aaaaaataca tttactaaag tcggtaagac ctctgaagac
     3721 cgactctttc aatattacct cttcgttgaa aacacaaccg gtgaacacca cactctttgc
     3781 aaatgaggtt ttaatgtatt ttttttcggc atcgaagccg gctgctatta gcttaccatt
     3841 aaaggtgcag tttataaagc tcaatggttc atcaatatac gccctgatgc ttgctttgtt
     3901 ttcgtgactg cggtttttca gtttcgtcaa atcaaggtcg ccggtgataa ttttatcttc
     3961 gagatatacc ggttctccgg agttaatcat ttttatcacg atatctgcat cgatttcttt
     4021 acccttggaa tcgttctgca ccgaaaggtt tccacacgac accaaaacaa ccgacatcaa
     4081 agcgaacaaa agtattccgt gcttcataat ttaaaagttt tatcaaacaa tagtaatcaa
     4141 atgtagcatt ttcagcgcta aaatacaact caatttatca acatctattt aatgcagtat
     4201 tgcggcatat catggcaatt atttcatgcc aataatctta aactccgttc tgcggtttaa
     4261 agccctgccc tcttcgctat cgttggtggc aataggcata gaataatcgt agcccttata
     4321 gctcaaccgc gatttggcaa tgcctttcga aaccagatag tcgtacactg ctttagccct
     4381 gttttctgat agactccggt tgaaatcctt cgatccgaca ttgtcggtat gtcctcctat
     4441 ttcgatacgg aagtgcggat tgtcaatcag aaattttatt accacattta attcggcgaa
     4501 cgatgtttct ttcaaatcga atttattgaa gtcgaagaat atattcttca aaacaacggt
     4561 agttccctcc tttaccggct gcaacgggat gttcatgata tagggttttg acgggtcttc
     4621 gaggttcgac agcgaaaaat tctccgaaaa aaagaggtag ccctgtttgg atacattaaa
     4681 agcatagtca cgatcgattg gcagacacac aaggtaatta cccgtttgcg catcgctggt
     4741 ttcttctgca accagaattt cggtttcaag atcgataagt ttgcaatcgg ctttcagtcg
     4801 atttccggtt tccacatcat acacatatcc ttttacatag ctgaccgtga ccggttttgc
     4861 attaggaggc agattaaatt tgtatatatc gagcccttta cctctttcac gggcactggc
     4921 aaacatggcc acatcgcctt tggcattcac aataaggctg cgctcttcgt tttgagtatt
     4981 tataggatat ccgagatttt cggggtttcc ccaacctttt accgggtctt tccgtgaaag
     5041 gaacaaatcg tagctgccca gtccaatatg cccgtcagat gcaaaataga gagttcggtc
     5101 atcagggtga ataaacggcg agcactcatt gaattcagta tttataacgg gaccaagatt
     5161 aacggcatta ctcaccggaa atccatcagg tccgagcgct acactccaga tatccatttt
     5221 ccccatccct cctcctctgt tgctggcgaa atacaaggtg cttgcatcgg ccgataaact
     5281 gggattggaa tcccatgaac ccaaattcac cattttcccc aggttacgtg gtttcgacca
     5341 cccctgtgga gtaatatatg aaatgtagag gtcgcacgac ccgtaaccgt caggcatatt
     5401 gcatgctgtg agaatacaaa ttttaccgtt ctgggcaatg cactgcgcgc cttcgttgta
     5461 ctcggtattg gtttcacccg gcattttata cgcaggcata taatttccat ctttcaaatg
     5521 agaaataaaa acgtcttcct gatattcacc agaagcatta ccatactcat cgccggggat
     5581 gagccgagta aaaaccagta tttcttcatc ggcagtaagg cttggccagt attcatcgta
     5641 ttttgtgttc acaccgtttc ccagatttat aggctcaaat gcactgggat tatcgtatgc
     5701 aacaatggcg aattcaaccc gtttgagcaa atattcggtt ttggacttct cttcttccgt
     5761 taaatctgca ttttccatga aaaaagagat atgcattttc gattcatcgt atcttccggt
     5821 tttatattca tattgcgcca gtaccatgta gtaattcagg tcgtaatacg ggtcgatctg
     5881 tattattttt ttcaacattt ctgtcgattc cttatattga gcctgagcgg taaatatttc
     5941 attcataaga acataggctt caataaaagt actatcaatt tcgatggctt tgtaaaggag
     6001 ttgcaatgca tcgccgtttt tgcgattatt ggcaagctct agtgcagttt caaagttttt
     6061 tattgccttt ttcgattggg tatgatagga ttgtttgggc tgcgataagc ttacgcagga
     6121 acaaaaaagg ctaagaaaaa gaaaaaccgt tattgttttt gtcatggtat attcaagtat
     6181 ttatgcgatt gtaacgaaat tctccattgg gggttttgtt tcacatattc ggcaatagtg
     6241 ttggcatttt tgcgcaaacg actccattcc ggctgaagga ataaatgaca atcgtgttta
     6301 acctgtgatg cacaatgttc agcccagttg aagtcggatt cttcgtaaat aactattttc
     6361 agttcatcag ccattgtaaa atttgccggc accggtggtt cctgtttttt gggtgacagg
     6421 caaatccagt gccattttcc tgaaacagga tatgctccgc tggtttcgag ataacatgca
     6481 ataccatgtt gggcagcaat acgggtaaat cctgtaaaat cgtataacat cggttctcca
     6541 ccggtaacca caaccgtacg cgctgctgtt tgcaatattc tttcaatgat ttcgctgacc
     6601 ggaatataag aatgaacatc aatatcccaa ctctccttac tgtcgcacca gcggcatccc
     6661 aaatcgcaac caccaaccct taaaaaatag gctgccttgc ctgtgtgaaa gccttcgccc
     6721 tgcaccgaat agaattcctc gataacaggt attgaatcaa tggatttgct ctttaacaaa
     6781 ataatctatt taataatctt atccaaaata ttccgtgccg cgtttttata taccatgatg
     6841 ttcatcattt taagctttac ataatcttca tggtaaaagc gatagccttt atttgggcca
     6901 tcgaaacctc cgctgccgga gtcttttatc aggtaccagt aatcgtcgcc aactttctga
     6961 tatcctacta tgtgtatgca atgatcgtcg gtggtgctgc cattttcgag ccgtagctgc
     7021 cgtgattttt cgttgatcag ttccgaaggc acatcaaaat cggggacaac agcaacttca
     7081 gcgtaactgt cgtagccagg ttctgttaca tcgccgcaca ggcatacggt aaaatcgttg
     7141 gtaatggcgt cgttaattat tttcataaaa tcgtcgggag taacattata ataatcttcg
     7201 ctgtgccacc agttgtcgtc ttccacaagc tcatgctttt cgttgtaagg gaatgccatt
     7261 gttgacataa agctgaaata atcggttggt ttcagaaaca aatattcgct gagatacgtc
     7321 tgaggtgtat attttttccc ttctacaata aactccacag gaggtttccc catatatttg
     7381 tcgaggatac cacggatgtc tttttctgct gttgcaaggt cccaattgcc cgattccttc
     7441 attttttcga gatactcatt catttcgtcc accatagcat cgtgggagtg aaatttatga
     7501 tttccggtga gtcccgaata agctgtttcg ggaacaatgc catacacagg ccagagcctt
     7561 ttaattgcat tggcttcgct accctgcgca aaataggttt ccccttttgt gttcacatag
     7621 tctttggcac gatcgacata ttcccagtac acggtgtaca tttcagaaag gtccggctgc
     7681 agcccgtgaa gccggtaagc ttccgattct ataaatgagg taaccgcaaa acaccagcag
     7741 gttcctgtgt ttccctgcga tttgggtgca ctgtgccagg agcgcgtgta ttttttaata
     7801 tccgtcggga attccctgcc gctgaaatcc atagtaaaac gcttcggaaa gctggtgcgg
     7861 acaaaatcag gatttatatc tttcaggatt acattatcgt aaaaacctgc cttaaaatcg
     7921 ctgtaaacag ccttatcgtg actttgcccg aataatatac aagccgaaag aagaagacct
     7981 gaggtgatga acaatctttt catagtatat gttttatctt tttagtacaa caatattgcc
     8041 actactcacc gcaaacttac acaaattagc agaaatttac cttacaacat ggcagtaaat
     8101 aaatcgagta tagaaattat ttcttacgta ttttcataat tcgggcatac aaactgttcc
     8161 agcccgaagt atttttttcg tactgcaagg gtttgtacga aacaattttt tcgatatcat
     8221 acgttcttaa tccgaattgc gcgagaactt ccgctgcatt ctggggaatg gtttcaatac
     8281 aatcctcctg atacagttgg agcgcatgtt cccttgagat ttcgccatta cgcaccatat
     8341 tgctgaactg gcagagccgc ctttcgtagc catagatccg gtttctgata taattcgtca
     8401 gtggttcagc aatacaatcg agatgtttcg aatactctct tgaatcgtcg cgccaaccca
     8461 tagtttgact gataatacaa cctatttcgc ggtctgtaac cggattcaga taaaataagg
     8521 gtcgtatttc tctggcaaat gctcctgtgg attttctgac cgataaaacg aagttgttca
     8581 gattcgggta aataagaaat ttgttgattt cgctgttgct gaattctttc gacttcctga
     8641 taatatattt gaaccgtgct acatcctgaa ttgagtcggg aacgaaggag tcttcttcaa
     8701 gcggtgaagt accgtagagc acatagggaa tgcggtaatc gcacgcaacc ttgattatat
     8761 tggcttttgt ggcaatatcg caggtaccgc agatatcacc tgtattcagc aacatataac
     8821 catatatttt attcagcaac ttctcgtcgg gacggcacca tacatgatca acacctgtgg
     8881 cctgaagcgc aaggttgatg ttagctctgg caatgtcgct gaataaaccg ttgtcgaagg
     8941 tcatcgtaag caccctgagc ttatagacat taacagcaag gtgcaggatg tatgtactgt
     9001 ctttcccacc gctcagggga accaacacat cgtaaggggc cttttttgct ttggcatttt
     9061 ccagcaattc tagaagcttg ttttcgccta taggttgaaa agttttttgt tttgagcaga
     9121 gagaacaggt tccgtcatgg tcaaaggtca aatgcgggta attactgcta atgatacaac
     9181 ggttgcaagt ttgcattgag aataaataga gatgatgaaa tcaattaata atttttcctt
     9241 ttaaaatagc ggtatcaatc aggtctgagc cataggcata cggaataaat tcaaggctgg
     9301 gtataggctt ggtaatgata atgttggcca gtttgccggg acatatacta ccggccacat
     9361 cacttacacc catagcgtag gccgagttga tggttgcagc gttcagtgct tcctgcggca
     9421 gcattttgta attcacacag gcaaacgaca tcatcagttt catatttccc gaaggcgaag
     9481 agcctggatt ataatcggat gcaatggcaa ccggaagtcc tgctttaatc atttcgcgag
     9541 ccggtgcaaa aggcatattc agaaagaatg ctgcccccgg taatatagtc ggcatagtgg
     9601 tgcctgaaag caaagcagcg atttcttcct tccctgtata ttcgatatgg tcaaccgaaa
     9661 gtgcgctata tttaacacca acctgaatgc ctcccgattt tgccagctcg ttggcatgga
     9721 ttttcggccg cataccgtat ttaattcccg ccatcagtat tctttcggtc tgttcagtag
     9781 taaaaaagcc cgtatcacaa aagacatcga tatagtcagc gagttcttca gccgctacca
     9841 ccggtatcat ttcgttgata atcaggtcga catattcatc acggttgttt ttatattctg
     9901 ccggaaaaga atgggctccg agaaaggttg acttcaccac caaaggagta gtatctttaa
     9961 tgcgtttaat cacacgcagc atcttcagtt catcatcggt attcaatccg tatccgcttt
    10021 taatttcaac ggcaccggtt ccctgcgata ttatttcgaa cacacgaact atgctttgtt
    10081 catataattc gtcttcgcta gttttgtgaa gcaacgcagc cgaattcagg atgcctcctc
    10141 cccttttagc tatttcctca tagctcaatc cctttatctt atcagaatat tcaatttcgc
    10201 ggcttcctgc atatacaata tgcgtatgcg aatcgcaaaa cgaaggcata actgttttcc
    10261 ctgaagcatc aatttccgta gttgaagcat cggttataat gcttgtaaac ggatatgctt
    10321 ccatccggcc aaaatccttt atcagatcat cctcgaacag aagccatgca ttctccaaaa
    10381 caggaacatt tgccatttct gctcctttta ctagcattga gcatttgcgt tcggcctgaa
    10441 caaggtacct gatgttttta ataattgtct tcattcattt cgtctttagt aaattattgc
    10501 attgcacgtc aatcagttcc tgtatggtaa aaacacaata ttgcctttta ccagtccctt
    10561 tttatcacct tcgtttgcaa taagtaaatc accgttttcg aaaaaggcga taccttcagg
    10621 tttaaagtaa atatcagcgg gcaaaggata aaaccgcaga atttttccat ctttacaaac
    10681 cactaacatt ttatcgattg aagaaagtat gtaaagctcc ttagtaaccg gatgcaccgc
    10741 aatggctgac ggattaaaac tcactttacc tgaatagata ctaccaaatt gggatgcaaa
    10801 caaacggttc agctccagtt gatcaattac aactgcagat tcaatttctg atgcttgacc
    10861 gggtaaatac ttgaaaacta agcgtttggt ttcatcgcca cccaaaagat gctccttact
    10921 ggccacataa aaacaatttt gtgttttatc atagtacaat ccttcgagat tcagagagtt
    10981 aataggcaga atagattgat ttacagccga attgttatga taataatcaa aactgaagag
    11041 ggttccgtcg ctgcgtaaca caaatacgtt attccctgca attgcaatat cttcaaaatc
    11101 gccaacatcg gtaaaacgat gcattttggt aatactctcc gaaattaggt taaactcaaa
    11161 tacaacgccc agctcatcct gaatacatat caccgattcg ttagtcaatt tagcgatacc
    11221 agagagctcg ttcaatacat cgggcatttc gaaaacactg tcggtattga aaaaatcata
    11281 gggagagttc ttgtataaag ctatttcgga cagtttcgaa gaattccact tatcggatac
    11341 ggcaatagat ttcgatttat agaatttacc tgatgttgtg aaatacacgt tctgctctat
    11401 accatcgcgt ataatttcaa ctttcaggaa cgaagtgtcg ggtaactcca ccaatgatat
    11461 ttcatcgaga atccaaccct gatatttttt ttccaacttt ttattgattt tatcaatggg
    11521 gacaaccgaa atatcagccg gtgattcagt aaatatgatc tgaccattct taataccagt
    11581 ttcatataac gtgttcccgc agaaatagtt tacttcggta tactctttcg actcttcaat
    11641 ttcaacaata tcggcatcag ggcatttttc atgtattagt gccattatac ctttatcgtt
    11701 aattgtgagc gattggcggc ggttgtcctg acttctgcag gaaaacaacg ccaaatacca
    11761 acaagttagc aagaaaaaag ccaggtattt catcaattct aaatctgttt tattcgcaaa
    11821 agtgccaaaa taaatttcag aaagcaaaca taagcgtttg aatttaacat caaacaaagt
    11881 tgaaaagtct aagcctttat caataaatat tgattaaata ttttactaaa tttgcacaaa
    11941 acaatgcctt atgaaaacat tagtatttgc tctgtcgtta ctgattattg cttcgattgc
    12001 aacagctcag gaaaacacca gccctgtttc tgataaaccg gcggatgact ctgtgtttat
    12061 cacgcagcat ttattgggat tgaatgtttt ccccgctttt ggaatacttg ggggtggtat
    12121 tacgaataac tccaaaatta ctttgcaata taaaattctc acgaagaagt ttaactaccg
    12181 tttcagcctg aactacctta atttttaccg atcgcacaat gcgttcgaca ttgtaggcat
    12241 tgttgccgac actacgcttc ctgcctctat tggagatgac accatatttc agagcctgaa
    12301 aataagaaat tatttcaatc aggtaaacac gtatgatttg cgatttggtg ccgaatacgc
    12361 aatacagcgc gacgacatca gattttacat cggtctgggt ggcataatag gaatccataa
    12421 tattgtgaac agctacaact atttttacaa gccttgtccg gaagaaggtt atcctgttga
    12481 taatctgtat tttggatatg agtacttaaa tgaacttgat aatcacagca gagctacaag
    12541 ttttttaaaa atgggtgccg atctttcgct gggtgttgat ttcaatctag ccgacaactg
    12601 cgttgttgca ttgcaatata cccctgaatt tgcatattac agatggttga gtgacaagaa
    12661 aaccaacgat actgatgatg tctttcctaa tcgtttgaac agtttctggg ttttctcccc
    12721 cgatttcatc gatttgctgg tgtatataag gttttaacgt acttaccacc atacgtgctt
    12781 ccttgccgga ctaacaatat agggtaaaag ggtgcaaata cgtcatttgc taattttcgc
    12841 agattttact ctttaataaa acgcgtcgca atatttttac cgttaatagt tgcattaata
    12901 aaatacaatc cttcaggata tccggtaagg tcgaaaataa agctgcccga aaaatgtgct
    12961 tgttcaataa cctgacctaa cgagttaaca atttcagcaa caacaaattg taggtttccg
    13021 gcagcggaaa cggttatttg gtcgcgtgca gggttcggat acacacctat tatcgaaccg
    13081 gataatccat taaaaactga actgtttata attgaaatta cattggatgg gtcggatgca
    13141 caaccattta cggtaacgat aacatagtag ttaccatcgc cttcataatc aaaaaactga
    13201 gagttcgctc catcgatagc tccatcatcg ttataccact gattacccgt gccggagttc
    13261 gatataagcg ttgcacccga ttggataata ataggtgtag gtggtttctg attcacagta
    13321 atttcgagaa tgacagcatc gctggtgccg caatcgttta cagcagctac cgtaatattg
    13381 cctgaaacag cccatatacc atagctgatg taaatttcgt ttgtcgtact agcaccgtat
    13441 gctccgttag gcagcgtcca aatatacgag agtgcatctg tttgcgggtc aactgaataa
    13501 tacacatcgc cctgcccctg acatactacg ttgttcccat taataattgc aggagtttct
    13561 acaacatttt ttactgttac cataaccgta tcggaaacag ctgcacaaac tccattttgg
    13621 gcaacaacca catattcggt tgtttccata ggggtaacag taagctcggt aagctcgtct
    13681 tctgaaccgg aaatatacca ggctgctgtg ccatttgtcg atgcagaaag agtaatcggt
    13741 tcatactgac atatttccat atcgtccatt acaacaagcg tagtgagctc atacactgta
    13801 acctgaatgt aagccgtatc ggagcattgg ttatcgtcaa taaaaatata atgcagcatt
    13861 tgagaaccgg cttcgggatt aaaattattt tcttcaatgc catttccctc ataaaaacca
    13921 ttttccggag aaccacccat taaggagacc atgccattat caatgcaaat cgggtcaata
    13981 gccgaatggg ttacctcagg agcaatatta acaacaatat cattctcgtc ggagtattcg
    14041 caaccataca tgtcggtata tgtataagta atcgtttgag agccgctggt ggggtccaga
    14101 aaattgtttt ccactcctgt tcctgaatac gtaccccctg aaggcgtggc agtgtttagc
    14161 agaatatttc cgatattact gcatattggc tcgaactcgg gaaatacaat tgggtttgca
    14221 gggcaattta cctgaaaaat gtagtcctct gattttgagc aattataatc gcttgcaact
    14281 gtcactgtaa aattgtaagt acctgcaagt gttggcaaac cagaaagcac acccgatgtt
    14341 gacagcgaaa ttcctggagg caggttgcca tcggtaactt cgaaacttgc ggggcctaaa
    14401 gctcccgttt gcgtaatcag tgcagagtaa acagtaccgg ttgttccgtc aggtacggaa
    14461 cctagggggg ataatgttac cgctgggcaa acaaatggag tatatattac ctcatcaata
    14521 ccaatgtaat cggaatttgt acctgatggg ccaccgttgg tgacaaaata tctgaacgca
    14581 aagcgacctg acacaggggc gcttaagcct gtaattgtta ttgaataaaa cccccacgac
    14641 aagtaagggt acacactggt taccagcgtg ggattaatat caagcaacaa cgtggtaaaa
    14701 tcgccaactg ctgcagaacc tgttcctaca ttggtgctgg caccattcgt actcattctt
    14761 acctgaagcc ggtcgggata agtgttatca tcagtagtac gggtataaaa gctcaagaca
    14821 tcgccattac taatggttac tgagggtgta accagccagt tgctaatggt attggcaccc
    14881 gttgtgttgt tgaaattggt acctatataa gcggttgccg cccccgattg tgccgaaaac
    14941 acatcagagt taccctggaa ccaattggtt gaacccagtg gcacactgtt gttctgcatg
    15001 aaccagccag agcctgtaag tgttgttatg tcgttaaatc cctctgtaaa cgcctgtgca
    15061 cgaagcgaca tgggctcata caacattgcc gcaattagca aaacaaagaa aaatttgttg
    15121 aaatgtctca taattgtatt gggtttagta tttatgcaaa tataatcata aaattggtta
    15181 tttttatcaa ccaatttaac ccctaatttt ttaaaactct acttccatac tccagaaata
    15241 cccgttccac actttgggca tttcccgata aaacttggtg caatttgaat gttgtaagct
    15301 tttcggtcaa ttatctcaaa accgcatttg gggcaagagg taaattcagc ctgattttgt
    15361 acattaccca tatacacata ttttatcccc gctttacgag caatatcaca ggcttgctct
    15421 atagtttcaa ccggagtggg cggtacatcc ttcattttat aggcaggaaa aaaacggctg
    15481 aaatg
//
LOCUS       DAOOQP010000071        15082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4836_length_15082_cov_4.885539, whole genome shotgun sequence.
ACCESSION   DAOOQP010000071 DAOOQP010000000
VERSION     DAOOQP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4836_length_15082_cov_4.885539"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLK75_08255"
     CDS             complement(<1..180)
                     /locus_tag="PLK75_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOY39277.1"
                     /translation="MKSKQIIFCSLIFLTLISCTHKPVKVKSIYQPKPYVELTHPEWS
                     KNATIYEVNIRQYTPE"
     gene            complement(208..1293)
                     /locus_tag="PLK75_08260"
     CDS             complement(208..1293)
                     /locus_tag="PLK75_08260"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOY39278.1"
                     /translation="MNSLKIGNLKVRIPIIQGGMGVGISLSGLASAVANEGGIGVISS
                     AGLGLIYRHTANDYMEACIIGLKEEIKKAKEKSTGIIGVNIMMALTNFSDMVKTAIAE
                     KVDIIFAGAGLPLDLPKYLTEGCSTKLVPIVSSARAAKIICEKWKTLYNYLPDMVVVE
                     GPKAGGHLGFKTNQIDDPDFSIENLIPEVVRELAIFEEKYNQEIPVVAAGGIYTGEDM
                     YKIMQLGAKGVQIASRFVATFECDANIKFKESYLNATDNDVEIIKSPVGMPGRALKGI
                     FLDRVKQGLTAPKSCPYNCLHTCDYKKVPYCIMLALFNAYKGKMENGYAFAGTNAYLA
                     NKIVSVKEVINELVFDFERTRLAVLTV"
     gene            1585..2385
                     /gene="mazG"
                     /locus_tag="PLK75_08265"
     CDS             1585..2385
                     /gene="mazG"
                     /locus_tag="PLK75_08265"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOY39279.1"
                     /translation="MNPEYKEHTNEEKQQQFERLLNIMSRLRAECPWDKEQTNESLRT
                     LTIEEVYELADAVHTNKPTEIKKELGDILLHIVFYSKLGEEQGEFTITDVLKSINEKL
                     IYRHPHIFGDVSVADAREVEKNWERLKLKEGKKKTVLGGIPKGLPAMIKAYRMQDKAR
                     GVGFDWEKKEHVWDKVHEELKELQAEMEKSDVDNMEKEFGDLFFALINAARLYGINPE
                     NALEKTNTKFFNRFNYLEEQTIQQGKELKKMKLEEMDKFWEEAKNKGL"
     gene            2425..3543
                     /locus_tag="PLK75_08270"
     CDS             2425..3543
                     /locus_tag="PLK75_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39280.1"
                     /translation="MPIYTMWLFGLFSFMCVFQVLTYLLLFRKSTCNKTTVPGMAVPV
                     SVVIAARNESENLRLLIPALLNQKYPDFEIVVIDDRSEDDSYEIIREYMHKSDKIICV
                     RIDAESSELRGKKNALTAGIIRAKNNLLLFIDADCLPVTDMWISCMVSGLKPECEIVL
                     GYSGFYSDKSFLNEVIRYDTVFIGMQYSSFACAGLPYMGVGRNLLYTKSLWQMNNGFT
                     SHADLISGDDDLFVQQVANRVNTAVRIQADATTRSKAAQSFIGWCRQKSRHFSTAGRY
                     PITVGLFLVSEPVTRILYWLSFLTLLFTLPDILKILLISMWIFREMVTIKITCSFCKI
                     TGEPCSWVKIPFYNLILPFIYIFLWAFNKSQKGNYHWK"
     gene            3540..4136
                     /locus_tag="PLK75_08275"
     CDS             3540..4136
                     /locus_tag="PLK75_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY39281.1"
                     /translation="MNEHFSQKAQEDIVLINSALNGDGSAYTKLLNKYFDSIYYMLLR
                     KVNSVVEAEDLAMETFGKAFNNLVQYTPEFAFSTWLFRIANNNCIDYLRKKRAKTFPI
                     DTSVESSDSDNMPPILANSPNPEESIIKEQKALFLLELIKKLKPRYAKLIELRYYQEL
                     SYEEIGKELDLPIGTVKAQLFRARELLYPLIKFKAENI"
     gene            4136..4759
                     /gene="rsmG"
                     /locus_tag="PLK75_08280"
     CDS             4136..4759
                     /gene="rsmG"
                     /locus_tag="PLK75_08280"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOY39282.1"
                     /translation="MNLISVYFPELQHDRLVLLEKYAKLLIEWNAKINVISRKDIDSI
                     EERHILHSLSLVKLFSFSASSQIADVGSGGGFPGIPIAIFCPQIQVSLIESIRKKANV
                     LCDMVNALGLTNVTVENVRAEDISKKYDFVIGRAVAAFPDFYNIVKHLVSLKSRNEFA
                     NGILYLKGGDFSGELSGFSRAKIFSIQTVFSESYFETKKLIYLPFSR"
     gene            4868..5026
                     /gene="rpmH"
                     /locus_tag="PLK75_08285"
     CDS             4868..5026
                     /gene="rpmH"
                     /locus_tag="PLK75_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOY39283.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMATGDGRKVLARRRAKGRKKLTV
                     SDEGRPKM"
     gene            5159..6004
                     /locus_tag="PLK75_08290"
     CDS             5159..6004
                     /locus_tag="PLK75_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39284.1"
                     /translation="MKKILLIITCAISVGYMVQAQEIDMVSKRGVPILPEQGDIALGI
                     DALPVFKYLGNMFNASINNTIAWNFVGDLGSTNNIYLQYYLEDNASVRLAVRLGTQNL
                     VNQEFAVKDLPVPDPDILVTDKMLVSNTNLNIGGSYQMHRGKGRVQGYYGAGLMFVWQ
                     QSAQHYTYGNPITTEFNDPNTTDFGTNLTGTGRILDVYNVKRLGVVAHAFVGVEYFFA
                     PKLSIGGEFGYGLLAIRTVGGSITEERWTGTAIERQTTDIANDGILTLDNHNNGANIY
                     LKFHF"
     gene            6040..6504
                     /locus_tag="PLK75_08295"
     CDS             6040..6504
                     /locus_tag="PLK75_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39285.1"
                     /translation="MKKLFPVLLVMFIGVAAFLTSCEEETPTADPLPTSTIEGVVYAN
                     IDLTNAVNEKAPAGTKIFFRINANELVLNPLPGYTYETLQYETTVNSEGKYTIELPCA
                     THQNVNVAVTPNDFSAVRQINATTTDTHYFDGGVLNQTIRNGEKHYLDIMYF"
     gene            complement(6549..7571)
                     /locus_tag="PLK75_08300"
     CDS             complement(6549..7571)
                     /locus_tag="PLK75_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOY39286.1"
                     /translation="MMKYPMPEKKRHLVWLRIKQILIITCITLVLSEIALGIYFRIKD
                     RDLIEAETTDYPYFYFQLLPEAGLRNTDGFKTQYDRNKNPGITRIAVTGGSVAYGNKP
                     EETIAYFLEKQLQQTYPYKKFEVINAGVPAFVVEQEFIMIQLVLQYYKPDIIVSIDGY
                     NDLISGDINRYYPSPDAIPPHNWRDFRSIEYNSRKNSLSSRFFGLFSNIGRLADYLYR
                     RTANEQQLYENLAGNKTLIGLTYANRVGDIYDFCKSKNIGYFHFLQPVNWGLKSKLNE
                     ARNNCLQQIYKEMQLQLDTCYYVCNITPIFDTVKDIYLDECHVHPEGNKIIAKTIAVK
                     LESTIQ"
     gene            complement(7549..8541)
                     /locus_tag="PLK75_08305"
     CDS             complement(7549..8541)
                     /locus_tag="PLK75_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOY39287.1"
                     /translation="MTLPVNAQTLLEAFSSKTVLIIGDVMIDAYLWGKVDRISPEAPI
                     PVVSVTHRENRLGGAANVALNISELGAVPILCSVVGTDEKGNIFSQLLKQNNLNDAGI
                     IYDSSRKTTVKSRVISNNQHLLRVDDEVSNPISDSITEALFARVQKIVAEYKPNAIIF
                     EDYDKGVITPSLIAKVTQLAEENNIITAVDPKKRNFDKYYNVTLFKPNFKEFCDGLKC
                     NIEKSDFDSIRQYAIKFMQAHQIRIIFITLSELGVFIGDGQNFYHIEAEHRDISDVSG
                     AGDTVISTATLCLASGLDIYNTAKISNLAGGIVCERSGVVPISKSALLDEISNA"
     gene            complement(8582..9649)
                     /locus_tag="PLK75_08310"
     CDS             complement(8582..9649)
                     /locus_tag="PLK75_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOY39288.1"
                     /translation="MRLILIVILALFIVNGYSQIPSGYYNSAQGLAGTPLRAALHNII
                     DNHTQVSYSSLYDYFEQTDKKSNGKVWDMYSDIPGSTPPYEYSFSSSDQCGNYDGEGD
                     CFNREHSWPNSWFGGEVSPMYSDLFHVYPTDGYVNNQRSNLPFGEVGSTSWTSQNGSK
                     VGSSNYPGWTGVTVFEPIDGYKGDFARTYFYMTTRYYTEDASWPSNQNSMVNKCEIKP
                     VPMQMLLSWHHSDPVSQKEINRNNAVYGIQHNRNPFIDHPEYADLIWDPDYSATSATE
                     ISLTTVFPNPATERLYIVPPQSYPNLQLCIYNTTGELVFENQFTNETIIQLDVSGYKQ
                     GCYIIRLDNNNEINYFKVILF"
     gene            complement(9659..10702)
                     /locus_tag="PLK75_08315"
     CDS             complement(9659..10702)
                     /locus_tag="PLK75_08315"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY39289.1"
                     /translation="MKFSFLVILLLLVAFVTGFSQEKKEFEIVCVAFYNVENLFDTID
                     QPDVQDEEYLPTSANQWNSEKYFRKIERISTTLVKIGNEKTGDAPAVIGLCEIENSIV
                     LTDLVNSEAMKPFNYQIVHFDSWYLRGMDVALLYRPGYFEPENSVTHRIYFRNGVTFQ
                     SRDHLLVSGKLLGEPVHFVVMHWPSRRGGEKRSRQLRIESAELALQITDSIRKLDNDA
                     KIILFGDLNDDPFNYSIIKTLKVSNQRDIVSETNLYNPMYELYKKGVGSLAYRDSWNL
                     FDQFIVSSSLLNNESKSLIFYSAHIFNKPFLMQQDGRFKGYPWRTFVGNTYMDGFSDH
                     FPTFIYLIKEKTK"
     gene            complement(10699..11634)
                     /locus_tag="PLK75_08320"
     CDS             complement(10699..11634)
                     /locus_tag="PLK75_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39290.1"
                     /translation="MHRFASIDLGTNTCNLLLAGYEHGELMVQASEKRAIKLLRNSPA
                     QGLISDEGIALCIDAVMEYKNLILKYEPDVILANATSGVRSASNGLELLDKIERNSGI
                     HFTTISGTEEANLIFKAVKRAVNIENTDALLVDIGGGSIEFIVYYNNAAAWKQSFDIG
                     ISRVLNTFRFNDPLINNDMGRISQYFDTQLTDLYHILEQHNVKTIIGSSGTFETLSNI
                     LMHAGKSVESPLNTYRSIEIGDFKKVYYEIINSDFDKRMAIKGMEPMRVELIPLASWF
                     VNYLVDKYGIELIIHSRYSLKEGAIFAFIEKNNLK"
     gene            complement(11638..13032)
                     /locus_tag="PLK75_08325"
     CDS             complement(11638..13032)
                     /locus_tag="PLK75_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY39291.1"
                     /translation="MSKIGLIIQREYSTRVKKKSFIIMTILGPILFAAIMIAPALLAQ
                     LEDDELKTVAVIDDSYAFAPIVGEDGKVFHQVLKDTKYIKFDYLIGYDLDSAKKQLNG
                     SKYYALLYIPHNIVNVGTGNAQLFSYQQPNMALKMHIANCMEKHLEDIKLHKKGEELG
                     ISQQSIQEIIRVVNSNVEILTYKMEEDGKETKSSTEISMVLGYIAGFLIYMFVFMYGS
                     QVMRGVIEEKTSRIIEVIVSSVKPFQLMMGKIIGVALVGLTQFMLWVVLTFGLVTVAQ
                     AVLVDDVKMSQMASPAGDITGNTLTSSDQITDEKAEEFVEVFSSIKTINFPLIIGMFL
                     FYFLGGYLLYAAMFAAIGAAVDNETDTQQFMLPITGPLVLSIIIMVSAISNPNGSLAY
                     WFSLIPFTSPIIMMVRLPFEVPIQDVIISMSLLVVTFIFMVWLAAKIYRTGILMYGKK
                     VSYRELWKWLRHKS"
     gene            complement(13078..14013)
                     /locus_tag="PLK75_08330"
     CDS             complement(13078..14013)
                     /locus_tag="PLK75_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY39292.1"
                     /translation="MDFLIANEVEKKYGSFVALDKLNLNVPQNSIFGLLGPNGAGKTT
                     FIRIVNQITAPDSGGILIDGKKLSQIHINEIGYLPEERGLYKKMKVGEQALYLSRLKG
                     LSRNDAYKQLKYWFEKFEIAAWWDKKVEELSKGMQQKIQFIITVLHKPKLLIFDEPFS
                     GFDPINTNQLKQEILKLRYEGATILFSTHNMSSVEEICDYIALINHSKKVLDGNINEI
                     RNRYASGIYEFTYKGYFHKLAATLTPSYEILETSEVDGISNIKIRLLDKNISENELIQ
                     RMLGYGAVQSFSLVKPSMNDIFIRVVGESQKSESI"
     gene            14223..>15082
                     /locus_tag="PLK75_08335"
     CDS             14223..>15082
                     /locus_tag="PLK75_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39293.1"
                     /translation="MKRIILSTVLFALFSLLCFTGNAQFSIPALSTDYTISFDASVDG
                     VGNGVYAGTGFTNTPGVGQLDGNAWELNGMSDGNKLFDAEATTGDFARGTTLGSTTGG
                     FYAYEVETGNFAFGMQPGGTDVTPGYIALKVINNTGSEVYGIDFSYDVWVYNNAARSQ
                     KFNGEYSLDGTNWTAIPALALNTPEAADGTPAWVKTSLTSTFNASIADAGTLYVRWYT
                     DDLAGTGSRDKIAFDNVKIQMRAPEYVDWCNLSTPSLLTIIQGSTGNIYGEVLKTGVS
                     EAVGHGANIQG"
BASE COUNT         4617 a         2875 c         2926 g         4664 t
ORIGIN      
        1 ctcgggagta tactgccgga tgttaacttc ataaatagtg gcatttttac tccattcggg
       61 atgagtaagt tccacgtaag gctttggttg atagatactc tttactttaa caggcttgtg
      121 agtacatgaa attaaagtca aaaaaataag actgcaaaat attatttgtt ttgatttcat
      181 gatgtatgat ttgattaaat ttatatttta aactgttagt acagctaatc gtgttcgttc
      241 aaaatcaaat accaattcat taataacttc tttcactgaa acaattttat tagccaaata
      301 agcattagta cctgcaaatg catatccatt ttccattttt cctttatatg cattaaaaag
      361 agcaagcata atacaataag gtactttttt gtaatcgcag gtatgtaaac aattgtaggg
      421 acatgatttt ggagctgtca agccttgttt cactctatca agaaaaatac ctttcaatgc
      481 acgtccgggc ataccaaccg ggctttttat tatttcaaca tcattatcag ttgcattcaa
      541 ataagattct ttgaatttta tattcgcatc acattcgaaa gtagcaacaa accgactggc
      601 aatttgtaca cctttagcac ccagttgcat gattttatac atatcttcac cagtataaat
      661 tcccccggct gccacaactg gaatttcctg attgtatttt tcttcaaata ttgccagttc
      721 tcttacaact tcgggaatca aattttcaat agaaaaatca ggatcatcaa tttgattcgt
      781 tttaaaccca aggtgtccac cagccttagg accttcaact acaaccatat ccggcaaata
      841 attgtataaa gttttccatt tttcacagat aatttttgct gctcttgctg atgaaacaat
      901 aggaacaagt ttcgtactac aaccttctgt cagatattta ggcaaatcca gaggaagtcc
      961 tgcacctgca aaaataatat ctactttttc agcaattgcc gtttttacca tgtcactgaa
     1021 gtttgtcagt gccatcataa tatttactcc gataattcct gtagattttt cttttgcctt
     1081 ttttatttct tctttcagcc caataataca agcctccata taatcattcg ctgtatggcg
     1141 ataaataaga cccaatcctg cacttgaaat cacacctatt cctccctcat tggcaacagc
     1201 agatgctaaa cctgataatg aaattccaac acccattcca ccttgaataa taggtatacg
     1261 aacttttaaa tttccaattt ttaaactatt catttttgca catttatata attaatgtgc
     1321 aaatataggc aattaatgac taacttacag aaaaccttca aatctctacg ttatatttaa
     1381 ctctattttc tggcttctcg cttgttagtg tatatttata agtaaaattg ccaataagtt
     1441 gattcatttt aacattaaaa attttactac aaatatagtt tatttagata aaaacaacca
     1501 acagtgaata tcataaaaga aaaaatcgta attcagagat tagaattata tttgtaactt
     1561 tgtcggaaat aaaagaatga ttaaatgaat cccgaataca aagaacatac aaacgaagaa
     1621 aaacagcaac agttcgaaag attgttgaac atcatgagcc ggctcagagc ggaatgtccc
     1681 tgggataaag aacaaaccaa tgagagcctc aggacgctta ccatcgaaga agtatatgaa
     1741 cttgccgatg cggttcacac aaataaacct actgaaataa aaaaggagct gggtgatata
     1801 ttgcttcaca tcgtttttta ttcaaaatta ggagaggagc agggcgaatt tactattaca
     1861 gatgtgttga aatcgattaa cgaaaaatta atttacaggc atcctcatat ttttggagat
     1921 gtttcagtgg ccgatgcccg tgaagtggag aagaactggg aaagactaaa actgaaggaa
     1981 ggcaaaaaaa agactgtact cggtggcatc cccaaaggat tgcctgcaat gattaaagcg
     2041 tatcgtatgc aggataaagc cagaggggtt ggcttcgatt gggaaaaaaa agagcatgtt
     2101 tgggataaag tacatgaaga gcttaaagaa ttgcaggctg aaatggagaa aagcgacgta
     2161 gacaatatgg agaaagaatt cggcgatttg ttttttgctt tgattaatgc agcgaggcta
     2221 tatggaataa atcctgaaaa tgctcttgaa aaaaccaata ccaaattttt taaccgcttc
     2281 aattacctcg aggaacaaac catacagcaa ggcaaagagc taaaaaaaat gaaacttgaa
     2341 gaaatggata aattctggga agaagcaaag aataaaggct tgtaagtgta ctgtaataat
     2401 tacataacag tattaattgc agaaatgcct atttacacaa tgtggttgtt tgggcttttt
     2461 tctttcatgt gtgttttcca agtcctgacc tatcttttgt tattcaggaa aagcacttgt
     2521 aataaaacaa ctgttcccgg aatggccgtg ccggtatcag tggttattgc agcccgcaac
     2581 gaaagtgaaa atctgaggct gttgattcct gccttactga atcagaaata tccagatttc
     2641 gaaatcgtgg tgattgacga ccgctcggaa gatgatagct atgagattat cagggaatat
     2701 atgcataagt ccgataaaat aatttgtgtg agaattgatg cagagagttc tgagcttcgt
     2761 ggtaagaaaa atgcccttac tgccggaatt atacgggcta aaaataattt gcttcttttt
     2821 atagatgccg attgcttgcc tgttactgat atgtggattt cctgtatggt ttcaggatta
     2881 aaacctgaat gtgaaattgt gctcggatat agtggcttct attccgataa aagttttctg
     2941 aatgaagtaa tacgctacga tacggttttt attgggatgc aatattcttc ttttgcgtgt
     3001 gccggtttgc catatatggg cgttggcaga aatctgctct ataccaaaag tctatggcaa
     3061 atgaacaatg gttttaccag tcacgctgat ttgatatcgg gagatgatga tttatttgtt
     3121 cagcaggtgg cgaacagagt gaatactgct gtaagaattc aagccgatgc tacaacgcgt
     3181 tcgaaagctg cccagagctt cattggctgg tgtcgccaaa aatcaaggca tttttcaaca
     3241 gcaggcagat accccattac agttggctta tttctggtat ctgagcctgt aacaagaatt
     3301 ttatactggc tcagttttct aaccttactt tttactttgc cggatatttt aaagattctt
     3361 ttaatatcaa tgtggatttt cagggaaatg gtaactatta aaataacctg ttctttttgt
     3421 aaaataaccg gagaaccgtg tagttgggtt aaaattcctt tttacaattt aattttacca
     3481 tttatttata tatttttatg ggcatttaat aaatcgcaga aaggcaatta tcactggaaa
     3541 tgaacgaaca tttttcgcaa aaagcacagg aagacattgt tctgattaac tcagcactta
     3601 atggagatgg ttcggcctat acaaagctgt taaacaaata cttcgattca atttattaca
     3661 tgttgctgcg taaggtgaat agtgttgttg aagcggagga ccttgctatg gaaacatttg
     3721 gcaaagcttt caataacctg gtgcaataca ctcctgaatt tgctttcagc acatggcttt
     3781 tcagaattgc caacaacaat tgtatcgatt atttacgtaa aaaacgggca aaaacttttc
     3841 cgattgatac cagcgttgag agctccgatt ccgataatat gccacccata ctggcaaaca
     3901 gccccaaccc ggaggaaagc attattaagg agcaaaaggc attgtttctg ctggaactga
     3961 taaagaaact aaaaccgcga tacgcaaaac tcattgaatt gcgttattat caggagcttt
     4021 cttacgaaga aatagggaaa gaacttgatt tacccatagg caccgttaaa gctcagttgt
     4081 tcagagccag ggagcttcta tatcctctca tcaagttcaa agccgaaaac atctgatgaa
     4141 tctcatatct gtttatttcc ctgaattaca gcacgaccgt cttgttttgc tggagaagta
     4201 tgcgaaactg ctcatcgagt ggaatgctaa aattaatgtg atttcaagaa aagatatcga
     4261 ctcaatcgaa gagcgccaca ttttacattc cctatcgttg gttaaattat tcagtttttc
     4321 agcatcttca cagattgcag atgttggatc aggcggggga ttcccgggta tacccattgc
     4381 cattttctgc ccccaaattc aggttagcct gattgaaagt atccgcaaaa aggcgaatgt
     4441 cttgtgcgac atggtaaatg cattggggtt aacaaatgtt accgttgaaa atgtgagggc
     4501 tgaggacata agcaaaaaat atgattttgt tatagggcgt gctgttgcag cctttcctga
     4561 tttttacaac atagtgaaac atcttgtgtc gttaaaaagc agaaatgaat ttgcaaacgg
     4621 cattctgtat ttaaagggtg gagatttctc cggcgagctc tctggattca gcagagccaa
     4681 aatattctcg attcaaacag tgttctcgga aagctatttc gaaacaaaaa agcttatata
     4741 tttacctttc agtcgctgat tttataacag aaataagaaa aaatcaaaat tttcaacaat
     4801 cgtttgcatt agtaaaatta agttttatat ttgcagtccg aaataaatac ttataaaata
     4861 ttacgtgatg aaaaggacat ttcaaccctc gcaacgaaag agaagaaata aacacggttt
     4921 tcgtgaaaga atggcaacgg gagatggccg taaagtgtta gcacgccgta gggcaaaagg
     4981 aagaaaaaaa cttacagtat ccgatgaagg tcggcccaaa atgtaagaag aaatatttgt
     5041 aattgattgc aaaaagctgc tgtaaaggca gcttttttat ttgaaataaa aatataaaat
     5101 gtcttgtatt aataagaata aatgtatatt ttcgcaatac aaccaaaata aattcagtat
     5161 gaaaaaaatt cttttaatta tcacttgcgc cattagtgtt ggatatatgg tacaagcgca
     5221 ggaaatcgac atggtttcca aaaggggagt tccaatactt cctgaacaag gcgatattgc
     5281 tctgggtatt gatgcacttc cggtgtttaa atatctcgga aacatgttta acgccagtat
     5341 aaacaatacc attgcatgga attttgttgg tgatttggga agcaccaata atatttatct
     5401 gcaatactac ctcgaagata atgcctccgt taggcttgcc gttaggcttg gaacccagaa
     5461 tcttgtcaat caggaatttg cagtaaaaga tcttccggtg cctgacccgg atatccttgt
     5521 taccgataag atgctggtta gcaacacaaa cctgaatatc ggcggatcct atcaaatgca
     5581 tcgcggtaaa ggccgtgtac agggatatta tggtgctgga cttatgttcg tatggcaaca
     5641 gtcggcacaa cattatacct acggtaaccc gattacaacc gaattcaacg acccgaacac
     5701 aaccgatttc ggaactaacc tcacaggaac cggccgaata ctggatgttt acaatgtaaa
     5761 acgtcttgga gttgtagccc atgcatttgt tggagttgag tatttctttg cccccaaatt
     5821 gtcgataggc ggtgaatttg gttatggact gcttgcaatc cgtactgtcg gaggaagtat
     5881 taccgaagaa cgttggactg gaacagctat cgaaaggcag acaaccgaca ttgctaatga
     5941 tggtatctta accctcgata atcataacaa tggtgcaaat atttacctta aatttcattt
     6001 ttaataataa ttaattgtca aacttaaaaa tataaaaaca tgaaaaaatt atttcccgtt
     6061 ttattagtga tgttcatcgg agtagctgct ttcttaacaa gttgcgaaga agaaaccccg
     6121 actgccgacc cactaccaac atccacaatc gaaggcgtag tgtatgccaa tatcgattta
     6181 accaatgcag tgaatgaaaa agcacctgca ggaacaaaaa tttttttccg tatcaatgca
     6241 aatgaattgg tacttaatcc gctaccgggt tatacttacg aaacattgca gtatgaaacc
     6301 acggtgaatt ccgaaggaaa atatactatt gaactaccgt gtgctacaca tcaaaatgtg
     6361 aatgttgccg ttactccaaa cgatttttca gctgttcggc agattaacgc aactaccaca
     6421 gacacccatt attttgacgg tggtgtgttg aaccaaacta ttcgtaatgg tgaaaaacat
     6481 tatctggata ttatgtactt ctaaataagc ttaaacgaaa tgagaaaagc accgtaacgg
     6541 tgcttttttt attgtatagt actttcaagc ttgactgcga ttgtttttgc aatgatttta
     6601 ttcccttccg gatgaacatg acattcatca aggtatatgt cttttaccgt atcaaatata
     6661 ggagttatat tgcaaacata ataacatgtg tcgagttgaa gctgcatctc cttataaatc
     6721 tgttgtagac aattatttct agcttcattt agcttacttt ttaaccccca gtttacaggc
     6781 tgtagaaaat ggaaatatcc tatgtttttc gatttgcaga aatcataaat atcacctacc
     6841 ctgtttgcat acgttaagcc aatgagcgtt ttattgccgg ccagattttc atacaactgt
     6901 tgttcattgg cagtgcggcg gtataggtaa tctgccagac gaccaatatt tgaaaataat
     6961 ccgaagaagc gggatgacaa tgaattttta cggctgttgt attcgatact tctgaaatcg
     7021 cgccagttat gcggtggaat agcatccgga gaagggtaat atcggtttat atctccgctt
     7081 attaaatcgt tataaccgtc gattgaaaca ataatgtcag gtttgtaata ctgcaatacg
     7141 agttgaatca taataaactc ctgctctaca acaaaagcag gaactccagc gttgataact
     7201 tcaaattttt tatagggata ggtctgttgt agttgctttt cgagaaaata cgctatggtt
     7261 tcctctggtt tatttccgta agcaacacta ccgcctgtaa ccgcaattcg tgttattccg
     7321 ggatttttat ttctgtcgta ttgtgtttta aacccatcgg tgttccttag tccagcttca
     7381 ggaagtaact gaaaatagaa ataggggtag tcggttgttt cagcttcgat aaggtcccgg
     7441 tctttaatac ggaaatatat acccagtgca atttcggaca gtacaagagt aatgcatgta
     7501 attataagga tctgcttgat acgcagccaa accagatgcc gttttttttc aggcattgga
     7561 tatttcatca agtaaagcac ttttactgat aggaactacg cctgatcgtt cgcatacgat
     7621 acctcctgca agattcgata tttttgcagt attgtaaata tcaagacctg acgcaaggca
     7681 tagagtagca gtgctgataa ccgtgtctcc tgcacccgaa acatctgaaa tatcgcgatg
     7741 ctcggcttcg atatgataaa aattttgtcc gtcgccaata aaaactccca attccgacaa
     7801 ggttatgaaa ataatgcgta tttgatgtgc ttgcatgaac ttgatagcat attgccgtat
     7861 cgaatcaaaa tcgctttttt cgatattgca ttttaatcca tcgcaaaatt ccttgaaatt
     7921 gggtttgaaa agtgtaacat tataatactt gtcgaaattg cgttttttag gatcaacagc
     7981 agtaataatg ttgttttctt ctgccagttg tgtaactttc gctatcagcg acggggtgat
     8041 aactcccttg tcgtaatctt caaaaataat ggcgttgggt ttatattcag caactatttt
     8101 ttgtactcgg gcaaataaag cttcggtaat tgaatcgcta atgggatttg atacttcgtc
     8161 gtcaaccctc agtaaatgtt gattgttact tataacacga ctctttactg ttgtttttct
     8221 ggaagaatcg taaataatac ctgcgtcgtt cagattattt tgtttaagta gctgactaaa
     8281 aatgtttcct ttttcatcgg tgccaactac cgaacataag attggtacag cacctagttc
     8341 agagatattc agtgcaacat tggcagcgcc gcccaacctg ttctcacgat gggtaaccga
     8401 aaccacaggt attggtgctt cgggcgatat tcggtcgact tttccccaca aatatgcatc
     8461 aatcataaca tcgccaatta taagcactgt tttcgaagag aaagcctcaa gcaatgtttg
     8521 agcgttaacc ggtaaagtca tttatctata tttgaagtaa atatataaat atcattttta
     8581 atcaaaagag tatcactttg aaataattga tttcattgtt gttatctaac cgtatgatat
     8641 aacatccttg cttatagcca ctaacatcga gttgaataat ggtttcattc gtaaactgat
     8701 tttcaaaaac gagctcaccg gttgtattat atatacacag ttgtaaattt gggtacgatt
     8761 gtgggggcac aatataaagc ctttcggttg cggggttcgg gaatactgtg gtcaagctga
     8821 tttctgtggc acttgttgca ctataatcgg gatcccagat taaatcggca tattcgggat
     8881 gatcgataaa tgggttgcgg ttatgttgta tgccgtacac tgcattatta cggttgatct
     8941 ctttctgact tacagggtcg ctgtgatgcc acgacagaag catttgcata ggcacaggtt
     9001 taatctcaca tttgtttacc atactgttct gattcgaagg ccatgaggca tcttcggtat
     9061 aatagcgggt agtcatataa aaatatgtgc gtgcaaaatc gcctttatat ccatcgatgg
     9121 gttcaaaaac agttactcct gtccacccag ggtaattaga tgaccccacc ttagatccat
     9181 tttgcgaagt ccaggaggta gatccaactt cgccaaaggg tagattactc ctttgattat
     9241 tcacatatcc gtcggtgggg tatacgtgga acaaatcgct gtacatcgga cttacctctc
     9301 cgccaaacca ggaattgggc caggaatgtt ccctgttaaa gcaatcgcct tctccatcgt
     9361 agtttccgca ttgatcggag gaagaaaaag aatactcata cggaggagtg cttcccggaa
     9421 tatcgctgta catatcccat accttgccgt ttgatttttt atcggtttgc tcaaaataat
     9481 cataaagcga tgaatacgaa acctgagtat ggttgtcgat aatattatgc aaggccgcac
     9541 gcaatggcgt tcctgcaagc ccttgtgccg agttgtaata tcccgatggt atttgagaat
     9601 acccattaac aatgaatagt gcaagtatta caatcaggat aagacgcatg agtgctgttt
     9661 atttagtttt ttctttaatc agataaataa atgtcgggaa atgatcacta aacccgtcca
     9721 tgtaggtatt tccaacaaag gttctccagg ggtatccttt aaatctgccg tcctgctgca
     9781 tcagaaaagg cttgttaaaa atatgggctg agtaaaatat cagcgacttt gattcattgt
     9841 tcaataagct ggagcttaca ataaactgat cgaatagatt ccatgaatcg cggtatgcca
     9901 acgatccaac gcctttcttg tagagttcat acatgggatt gtacaagttg gtttcactaa
     9961 ctatatcacg ttggttagat acttttaaag tcttaataat actgtaattg aatgggtcat
    10021 catttaaatc gccgaagaga ataattttgg catcattgtc aagcttcctt atggagtcgg
    10081 taatttgcaa ggcaagttct gctgattcga tgcgcaattg ccggctgcgt ttttcaccac
    10141 ccctgcgcga tggccagtgc attacaacaa aatgtaccgg ttctcccaac aatttcccac
    10201 ttacaagcag atggtcacgc gattgaaagg taactccgtt cctaaaatat atcctgtgcg
    10261 ttacagagtt ttccggctcg aaataaccgg ggcggtaaag aagggctaca tccatacccc
    10321 tgagatacca cgaatcgaaa tgaacaatct gatagttgaa gggtttcatg gcttctgaat
    10381 tcaccaaatc ggtaagtacg atactgttct caatctcgca caagcctatt actgccgggg
    10441 catcccccgt tttttcattg cctattttca ctagagtcgt tgaaattctt tcaattttcc
    10501 ggaaatattt ttctgaattc cattgattag cactggtagg taaatattct tcgtcctgca
    10561 catcgggttg atcgatggtg tcgaacaagt tttcaacatt atagaaagca acacaaacta
    10621 tttcgaactc ttttttttcc tgcgaaaatc cggtaacgaa tgcaaccagt aagagtagaa
    10681 taactaaaaa agaaaatttc attttaaatt atttttttca ataaatgcaa agatagcacc
    10741 ttctttcaaa gaatatcggg aatgtatgat taattctatg ccatatttat caaccagata
    10801 gtttacgaac caggatgcca gtggtataag ttcaacacgc atgggctcca tacctttgat
    10861 tgccatacgt ttatcaaagt cggaattgat gatttcgtaa tatactttct taaaatcgcc
    10921 aatctcaatt gaacgatatg tattcaatgg gctctcgacg cttttcccgg catgcattag
    10981 aatgttcgat aaagtttcaa aagttcctga ggagcctatg atagttttta cattgtgttg
    11041 ttcaagaatg tgatataaat cagtgagttg cgtatcgaaa tattgcgaga ttctgcccat
    11101 atcgttgttt atcagcggat cattaaagcg gaatgtattc aatactctcg agattccaat
    11161 atcgaagctt tgcttccaag ctgctgcgtt attataatag actataaact caatacttcc
    11221 accaccgata tcaacaagca acgcatctgt attttcaata tttacagcac gctttactgc
    11281 cttaaaaatc aggtttgctt cctcagtgcc cgaaatagtt gtaaaatgaa tgccactgtt
    11341 tcgttcaatt ttatcaagaa gctccaatcc gtttgaagca ctcctgacac ctgatgtggc
    11401 atttgcaagg ataacatcag gctcatattt cagaatcagg tttttatatt ccataactgc
    11461 atcaatgcaa agagcaatcc cttcatcgga aattaaaccc tgtgcaggtg aatttcggag
    11521 tagcttaatg gctcgttttt ccgacgcttg taccatgagt tcgccatgct cataaccggc
    11581 caacaagagg ttgcaggtat ttgtacccag gtcgattgat gcgaagcggt gcatatatta
    11641 actcttatga cgcagccatt tccataattc gcgatagctc acttttttac catacattaa
    11701 aatacctgta cgatatattt ttgcagcaag ccaaaccata aaaataaaag taacaaccag
    11761 cagcgacatc gaaataatca catcctgtat cggaacctca aaaggtaacc ttaccatcat
    11821 aataatggga gaggtgaatg gaatcaacga aaaccaatat gccagcgagc cattggggtt
    11881 tgaaatggct gaaaccataa taattatcga aagtaccagc ggtccggtga tgggtagcat
    11941 gaactgctgt gtgtcggttt cgttgtcaac agcggctcca atggctgcga acattgcggc
    12001 atacaaaagg taaccgccaa gaaaatagaa taaaaacatt cctattatta atggaaaatt
    12061 tatggtttta atactgctga aaacctcaac aaattcttcc gctttttcat ctgttatttg
    12121 gtcggagctg gttagggtat ttcctgtaat atcacctgca ggacttgcca tttgcgacat
    12181 ttttacatca tccaccaaaa cagcctgtgc cacggtaacc agtccaaatg taagcacaac
    12241 ccatagcata aactgtgtta atcccacaag cgcgacgcca ataattttcc ccatcataag
    12301 ctgaaagggt tttaccgaag aaacaatgac ttcaatgatg cgactggttt tttcctcgat
    12361 taccccacgc attacctgag aaccgtacat aaaaacgaac atgtaaatga ggaatccggc
    12421 aatgtatccc aataccatcg aaatttcggt ggatgatttg gtttctttcc cgtcttcttc
    12481 cattttgtag gttaatatct ctacatttga attaactaca cgaataattt cctgaatcga
    12541 ttgctggcta atgccaagtt cttcaccctt tttatgcagt tttatgtctt ccagatgctt
    12601 ctccatgcag ttggctatgt gcattttaag ggccatattg ggttgctgat agctaaagag
    12661 ttgtgcatta ccggtaccca cgtttacgat attgtgcgga atatataaga gggcgtagta
    12721 ttttgatcca ttgagttgtt tcttagcaga atcgaggtcg tacccaatca ggtaatcgaa
    12781 tttgatatat ttggtgtctt tcagtacctg atgaaacact tttccatcct ctcctacaat
    12841 cggtgcaaat gcgtaggaat cgtcaatcac agccacagtc tttaattcat cgtcttcgag
    12901 ttgggcaagt agtgcagggg caatcattat ggctgcgaat aaaattgggc ccagaatagt
    12961 catgatgatg aaacttttct ttttgacccg tgtactgtat tcccgctgta ttattaaacc
    13021 aattttactc atgtctggtg ttatttgatg tattgcttag attcgtttta ttatatgcta
    13081 tatgctttct gatttttggg attctccaac cacccggata aatatatcat tcatgctggg
    13141 tttaactagg ctgaatgact gaacggctcc atatccaagc attctttgaa ttaattcatt
    13201 ttcactaatg tttttatcca gcagacgtat tttaatattg ctgattccat caacttcgct
    13261 cgtctcaaga atttcgtagc tgggggtcag ggttgcagca agcttatgaa aataaccttt
    13321 ataagtaaat tcatatatcc cggaggcgta tcggtttctg atttcgttta tgttcccgtc
    13381 aagaactttt ttcgaatggt taattagtgc aatataatcg caaatctctt caaccgacga
    13441 catgttatgc gtagaaaaca aaatagtcgc tccttcgtaa cggagtttga gtatttcttg
    13501 cttcagttga ttggtattga ttgggtcgaa tccgctgaag ggctcatcga aaataagcag
    13561 cttgggttta tgcaaaaccg ttataataaa ctgaatcttt tgttgcatgc cctttgaaag
    13621 ctcctcaact tttttatccc accatgcagc tatttcgaat ttttcgaacc aatacttcaa
    13681 ttgtttgtag gcatcgttac gcgagagtcc ttttaatcgg gataaataaa gggcttgctc
    13741 tccaactttc atttttttat acagtccgcg ctcttccgga agatatccta tttcattgat
    13801 atgaatttgc gaaagttttt tgccgtcaat caatattcca ccgctgtcgg gtgctgtaat
    13861 ctggtttact attctaataa acgtggtttt tcctgcaccg ttgggaccca ataacccgaa
    13921 tatactgttc tgcggcacat tcaaattcag tttatccaag gctacaaaag atccgtattt
    13981 tttctctacc tcattggcaa ttaaaaaatc catactcata aggtttaatt gtgcgaaaat
    14041 agaaaaattt aacaatttag tgcttaaggt agcttgggat aaacttaata atttcataaa
    14101 ttaacaccac aaaaaataaa gaagtgatat tttacttgtt tttttaatga ttaaaatatt
    14161 atatttgttt ggaggaaaaa tgcgtacgtt gtaaacatta aataactaat aaataactac
    14221 ttatgaaaag aattatctta agtacggtgc tgtttgcact gttttcgctg ctgtgtttca
    14281 caggaaatgc acagttttca attccggcat taagtactga ttacacaatc agttttgatg
    14341 catcagttga tggtgtcggt aatggagtgt atgcgggtac aggctttact aatactcccg
    14401 gagttggaca attggatggc aatgcatggg agttgaacgg tatgagcgat ggtaataaat
    14461 tgtttgatgc cgaagccaca actggtgatt ttgcccgtgg aaccacactg ggtagcacaa
    14521 ccggaggctt ttatgcctac gaagttgaaa ccggtaactt tgcttttggc atgcagccag
    14581 gaggaaccga tgtcactccg ggttatattg cacttaaagt gattaataac acaggcagtg
    14641 aagtgtatgg tatcgacttt tcgtatgatg tgtgggtata taataatgca gcccgtagcc
    14701 agaaattcaa cggtgaatac tctcttgatg gaactaactg gactgctatt ccagcactag
    14761 cgttaaacac cccagaggca gcagatggaa cacctgcctg ggtaaaaaca agtttgacct
    14821 ccacttttaa tgcaagcatt gccgatgcag gtaccttata cgtaagatgg tatactgacg
    14881 atttggcggg aaccggttca cgcgataaaa ttgcattcga taatgtgaag atacaaatga
    14941 gggcacccga atatgttgac tggtgtaatc tctccactcc gagccttcta acaattattc
    15001 agggttcaac aggcaatatt tacggtgaag tattaaagac cggagtatca gaagccgttg
    15061 gtcatggcgc aaatattcaa gg
//
LOCUS       DAOOQP010000072        14952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4888_length_14952_cov_5.016849, whole genome shotgun sequence.
ACCESSION   DAOOQP010000072 DAOOQP010000000
VERSION     DAOOQP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14952
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4888_length_14952_cov_5.016849"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PLK75_08340"
     CDS             complement(<1..1613)
                     /locus_tag="PLK75_08340"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39294.1"
                     /translation="MKKLVLLIFLGFFAAFNAMSQCTPDEGCLDLENPGEICPMVFDS
                     GYVNVPYEQVITFIPQATYNYSGMNMTVQKIVINSVSGLPAGLDWDTNAEEFIPTNPI
                     TRYCGVIFGTPTTEGEYPFQFAATVTVLIGSVPVEVPVTQETLGYEAKVVILPENMAP
                     PVAEFSASLTSSPGGEVINFTDESFNATTWEWTFEGAMNPTSTEQNPTTTWYTQGTYD
                     VTLTVTNTNCFPPKTHTLIAEDYITITSNVNPYYFQESAQICEGDLFLWQGETYNTQG
                     THFAYYTSIHGMDSIYELTLTVNPVYFFPETHVLCDGDLYSWHGTDYSTVGVYTATYQ
                     TSAGCDSIYELTLTVNPSYEFVTNQNICDGEFFEWRGNNYDVPGTYTDLYYTNNGCDS
                     VFVLNLTVNSLPQHVVVLQNPSDGILTSGNYGQISLSTSFVGTQYWVTMGGAYFTGEI
                     GGTGSGLSLGNSFPPGSYNIWSRNQYGCVLLQGTVSFVENTGANKIVANVTFGTPASN
                     FPANHVKVVLYRETTDIGNNEVVVFEDEQL"
     gene            complement(2012..5473)
                     /locus_tag="PLK75_08345"
     CDS             complement(2012..5473)
                     /locus_tag="PLK75_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39295.1"
                     /translation="MLELLDHSNYKLTDSMKEYYFKKRVISIKLIILLTVIGKICFSQ
                     EQITETVLSGHTDIVNTICYSPDGKLLASGSNDKTIRIWDVNSGKCIKTLRKHNLKAV
                     NSVSFSPDGKFIASASNSRTVKLWDLQEEKNTKNLYRHKKKPVYSIVFSNDGNSILSR
                     SSNSIILWDIKNDVAIKKIIEISEFSDKIIDLCRDGRFFAYYTDGNYIIIMKRVNNNF
                     SQAFNIDDSNRYAGTLDISSNGDYVAYVDRNNNIKIRDINNNPIITLSEKTIADNNIN
                     NETEDYINSICFSPDGNSIVSSGSKETKIYVVNSGDCIKTIPKSLKYVRFSPDSMHIA
                     CVYDKDVYIYKVFFRDKICVDGNCGKYFGQEINGKANGYGKIFYDNGATYVGYFLDNK
                     RQGNGQFLDSVGNRYTGNWAVNRRNGYGIQIFFSTQGKYDGFWIDDERSGRGLMFDNQ
                     GKYDGLWKQNLRNGQGKMTYKDGGIYDGEWMNDKRHGQGTMLYASGDKYEGNWINDDY
                     GIEGKITYKDGTFYNGELKSNRYDGLGKIIYPNGEAYDGEWKDGKKSGKGVMNYANGD
                     VYTGEWLDNRRNGFGLHKYNKSNQTFEGNWIDDQIANGLGTYTWPDGQKYVGEFNNGT
                     FDGQGIRTYSDGSTYEGTWKKGIREGNGTMIYANGDKYVGDWKSDMRNGFGVHSYNQS
                     KQIYEGNWVDDYVTGQGTYTWPDGQKYVGEFSKGVAHGKGKRTYADGSSYDGNWEKDT
                     REGKGTMIYANRDKYEGDWVNDIRSGYGTIWYANGKKYTGEWLDDKEYADLAINDSED
                     GKYISVELASGGKCTVWCDINCENRSYFIKEMECKEGYADGWCSFITSTLDGKPICGY
                     MGYMQHGKKHGMGREIFILENVEYFYEGDWKFNKKDGLGELNLPDLHYYGSFKNDKFD
                     GKGRLYFKKGGVEVARYIGNFSNDLPNGQGAYYGEDGNIYEGNWENGEKNGQGKMTFT
                     DGTIYEGELKEGSLTGFGAFTYSNGSKYEGYYIEGVISESVVITMPNGDIIKGPVKNG
                     ELDGNVTIYYKNGTTSYAKFSNGEIVSSSSTTSKTSNSVGSPCIIKIDENYDTKEIGY
                     YKLKIIKVKCNDNGYELDYLLYNYPKRDNSHEEGWYEYNNSLFFNDDCLYTEDFSEAV
                     NKVCHCK"
     gene            complement(5448..>6139)
                     /locus_tag="PLK75_08350"
     CDS             complement(5448..>6139)
                     /locus_tag="PLK75_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOY39296.1"
                     /translation="QGPVGPTGATGSTGATGATGSTGATGATGATGATGTTGAIGPIG
                     PTGATGATGATGATGATGATGATGSSGNNVLTIGQNYQGGIIFWLDESGQHGLIAAIN
                     DQSAGIQWYNGENLVTEASLNAVYAGKENTRRIINNQGVGNYAAQVCDDYAVTLNNEY
                     YGDWYLPSKYELNLLFLQKDNVGGFTSGYYWSSNEDDIDNAWFQDFYSGGQMNWSNRT
                     FAYHVRAIRSF"
     assembly_gap    6140..6239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6310..6409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6410..6974)
                     /locus_tag="PLK75_08355"
     CDS             complement(<6410..6974)
                     /locus_tag="PLK75_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39297.1"
                     /translation="MKNLLTFSIAILLTSIIYAQVPQKMSYQSIIRNEQGQLIPNNAV
                     GMRLSILQGSADGEPVYIETHTPITNGNSSVTIEIGNGVVVSGVFEDIDWSAGLYWLQ
                     TEIDPQGSNGYSITGTFQLISVPYALYAESSGSSISGPQGPVGADGATGPQGPVGPTG
                     ATGSTGATGATGATGATGATGATGATGA"
     gene            complement(6981..7451)
                     /locus_tag="PLK75_08360"
     CDS             complement(6981..7451)
                     /locus_tag="PLK75_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39298.1"
                     /translation="MRKLVLLLIFALPSVLHAQYAITSVGGNSEGAGGYTSFSVGQVA
                     YTYISSETGSVSMGVQQPYEIWETGISEFDNVNLSIATFPNPTSDYLILKIGDKENLN
                     IDYQLFDINGKMLYNGVVIEGETKIDMKLLDPGTYVLNLVTLNQSLKTFTIIKH"
     gene            7851..8978
                     /locus_tag="PLK75_08365"
     CDS             7851..8978
                     /locus_tag="PLK75_08365"
                     /inference="COORDINATES: protein motif:HMM:NF018880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY39299.1"
                     /translation="MKKFVSILMLLSPVLLFAQDYPVGYREISVYDSNRERDITAYAY
                     YPAVQAEENASPVEGTFPYIVFGHGFMMNQGDYDYLYNYLVPKGYFFVSLTTETGASP
                     DHETYAQDLAFVCDNFYDLMLDDVVFHDCVGPKCVVMGHSMGGGASHLASSYTQNMEA
                     IITFAAAETDPSAIDAAANANVPTIVFYGEGDAVAPPDENQIPIYNSSNSDCKILIGI
                     NGGIHCYFAESQWACDFGETMSGSDAGIEREEQHDIVTDLLGLYLDKKLKGNHASFLQ
                     FVDSVDNSSRITTQASCDFTEITTDELYQFEFYPNPTQDVIEINIASIDFEVSIINIM
                     GQTVKEVHNKKLIDVSDLKPGTYVIQVITGSRSMRNKVIIW"
     gene            9136..10839
                     /locus_tag="PLK75_08370"
     CDS             9136..10839
                     /locus_tag="PLK75_08370"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39300.1"
                     /translation="MQAIRIRQISFIIGLIIIAKSGFSQTDIPDSFNSYIDSLTELSL
                     NSPNPEVRVDALNKISTEYAHRNPVRIYKYAIDAIELANEINYTTGLIKAYYHLGDYY
                     YFQANFEEAQKYFWKSYSIANQHNIDSLAVDALNVIAVINASMGNYDKAIGYFEKIIR
                     ISQEKQFSSSENHAMINIGNCYREQGKNELAEKYYIRAYQQAKIRQANDEQIYSSHNL
                     AVFYGMTGNYPKAIMYCNEAIALAEKIQMNEQILEGQNILANIYIYQNQPGKAIEIAE
                     KALTRADSTHANLQKMHLYNKLMTANEQIGDYESALKWMKLEQTIKDSIFNIEKHFQI
                     TNLMEQYESNLKDLEIEKKNNELGRKNSLIRFILIILLIVALSATTIIIMFIRKRKTN
                     KILVKINHELSQKYQNYNIEFIPAISASELTSNGKSNFEENKNENNVKYVSSSLSDER
                     KRELLNRLNKLMSEDALYLDPEISIERVSESLQVNSKYLSQVINEGLGRNFPQYINEL
                     RVLESIRILSDPKQDKYSIEAVANMSGFKSKSVFNTAFKKQTGITPSFFRAESKNISI
                     N"
     gene            10943..>14952
                     /locus_tag="PLK75_08375"
     CDS             10943..>14952
                     /locus_tag="PLK75_08375"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39301.1"
                     /translation="MKRRVLLLSMVGLLILFSAQAQISLVKDIANAPVTGNSYPKEFV
                     ELNGNILFVATDPIHGSELWRSDGTTAGTYLLKDINPGSNGSTPQYFTKSGNKVYFKA
                     NNGSHSNELWVSDGTPDGTLMLKDINPGIDESYPGNFVDMNGILIFKAYTPEYGSELW
                     KTDGTPGGTVMIKDINPGTVSSYPGPGICVNNVVYFAAEDTIHGQELWRSDGTEAGTY
                     LLKDCAIGKRNGTPHAYVNLNNQLIFVAFDTIHGFELWKSDGTTAGTFMYCDINPGYC
                     DTTYDDIPSQLIVYNNKVYFQSAFESYGQELFVSDGTEAGTYMLKDIDPADASRPGNF
                     AIANNLLFFTADVNGYGRELFKTNGTAAGTVMIKDIWPGNTSGYPLALTAMGNYLFFA
                     ANDSLHGEEIWRSNGTTAGTVMVKDIYPGTQGSTPSTYGMGSSGEKVFFCATDGTIVG
                     RELYATDGTMAGTTLIKDIYSGSAGSEPLYLTALNGNVFFTAIVDTYGRELFISDGTT
                     SGTQLLKDIRVGSSSSSPLNLITFGNKLLFTANNGSSGTELWSSDGTTANTSMIKDIC
                     PWAGSSPQNFIQMGNYVYFDANDCNNGTELWKTDGTTAGTTMVKDITPGSNGSSFGTK
                     FLHNNHIYFNVWSNSIGFEPWASDGTEIGTFLLKDIAPGNRSGSPSKHCNLDTLMLFN
                     AYDSIHANELWISDGTEAGTHLLKDINTGENSSNPNNLTSLDSLIVFTAFQPETGTEL
                     WVTNGTSDGTHLLKDFSTGPSSSNLSNFFAFNNQIFFSAFQAATGYELWVTDGSIQGT
                     YMVKDINPGDYSSNPSDFVVIDNKLYFYANDGSHGIEVWTSDGSEVGSFLVSEGIEGI
                     DNLNARKMVANGSHALFCAQNSNSGYELWRFDPHYVSVTNVNTDSVCVGAEISVIFEA
                     VGTYNSGNSFVLQMSDSNGNFDNPTIINSISALESDTIIGNIPGTIDGGTYVFRVVAT
                     SPETNRGAISQSVVINPLPQISIDNVSPICINATPIELSHGQPSGGSYSGVGVSSNFF
                     NPALCGSGETSITYSLTQNSCTNTVTYSITVSPLPAANHSITGEQSICQGQQNVVYQI
                     EALDNTDSYAWTLPDGATGNSNTNSISVNFGLNAMSGNISVTGHNNCGDGQTISLPIT
                     VNPLPAANHSITGEQSICQGQQNVMYQIDALDNTDSYIWTLPDGASGNSNTNSISVNF
                     GLNAMSGNISVTGHNNCGDGQTISLPISINPLPAANHSITGEQSICQGQQNVVYQIEA
                     LDNTDSYAWTLPDGATGNSNTNSISVNFGLNAMSGNISVTGHNNCGDGQTISLPITVN
                     PLPAANHSITGEQSIC"
BASE COUNT         4563 a         3149 c         2511 g         4529 t
ORIGIN      
        1 agttgttcat cttcaaaaac tactacctcg ttattgccaa tatcagtagt ttccctgtac
       61 agtacaactt taacatgatt tgcggggaaa ttgcttgcag gtgtaccgaa agtaacattt
      121 gccacaattt tatttgcacc tgtgttttca acaaaactta ctgttccttg cagcaacaca
      181 cacccatatt gattgcggct ccatatattg taactacccg gagggaagct attgcctaaa
      241 gataatcctg atcctgtacc accaatttca cctgtaaaat aagcaccacc catagttacc
      301 caatactgag ttcccacaaa acttgttgag agtgaaattt ggccataatt cccacttgtc
      361 aaaataccat cacttgggtt ttgcaagaca accacgtgct gaggtaatga attaactgtt
      421 aaattcaaaa caaacacact atcgcagcca ttattggtat aatataaatc ggtataagtg
      481 ccgggaacat cataattgtt cccgcgccat tcgaagaatt ctccatcaca aatattttga
      541 ttggtaacaa actcgtagct tggattgact gtcaatgtaa gctcgtaaat actatcgcaa
      601 ccagcgcttg tctggtaagt ggcagtataa actcctactg tactgtagtc tgtaccatgc
      661 catgaataca aatccccatc gcacaaaaca tgtgtttctg ggaaaaagta aaccggattg
      721 accgtcagtg taagctcata aatactgtcc attccatgaa ttgaagtata atatgcaaaa
      781 tgtgttcctt gtgtattata agtctcacct tgccataaaa ataagtctcc ctcacaaatt
      841 tgagctgact cctgaaagta atatggatta acattgcttg taattgtaat ataatcctca
      901 gctatcaatg tatgagtttt gggaggaaag cagttggtat tggtcacggt caaggtaaca
      961 tcataggtac cttgggtata ccatgttgtc gtcggatttt gttcggtgct ggtaggattc
     1021 atagcacctt caaatgtcca ttcccatgtg gtagcattaa agctttcatc ggtaaagtta
     1081 ataacctctc cacccggaga tgaggttagc gaggcagaaa actcagctac cggaggagcc
     1141 atattctcgg gaagaataac aacttttgcc tcatatccga gggtttcttg agtaacagga
     1201 acttcaacag gaacagaacc aatcaatact gtaactgttg ctgcaaattg aaaaggatat
     1261 tcgccctcag ttgtaggtgt tccaaaaata acaccgcagt acctggtaat tggattggtt
     1321 ggaataaact cttcggcgtt ggtatcccag tcgagcccgg cgggaaggcc gctaacactg
     1381 ttgattacaa ttttctgaac agtcatattc atgccactgt aattgtatgt tgcctgtgga
     1441 ataaaggtaa tcacctgctc gtaaggaaca tttacatagc ctgaatcaaa aaccataggg
     1501 caaatttctc ccggattttc gagatcgaga catccttcgt caggagtaca ctgtgacatt
     1561 gcattaaagg ctgcaaagaa gcccagaaaa ataagtagaa ctagtttttt cataatattg
     1621 tattttgttt aattttttca atattagatt aatacatgcc atctttttta ataatttctg
     1681 caattcaaat tttcaaaatt aaagcttatc gaaatgtatt caaccaacaa tctatatttt
     1741 aatatttccc tcaaaattaa atcatttcct caataatcca agacaaaatc caatatctga
     1801 aatagatttt tcataggcag ttgttttgta cctgtttctc aagcgttttt gcacccgtgt
     1861 tgctttcaaa atgtgttggt ttgcgctgct tagtccggtc ggtatttgtc tgcaaagctg
     1921 ggaaaccgaa agctaagcga tttctctaca cccgagacct ccgcagtcac ttcacctaag
     1981 catgtggctt tttctgatgt aataaataca actacttaca gtgacaaacc ttatttactg
     2041 cttcagagaa atcttctgta tacaagcaat catcattaaa aaaaagacta ttattatatt
     2101 cataccaacc ttcttcatgg gaattatcac gtttaggata gttatacaac aaataatcta
     2161 attcatatcc gttatcattg cactttactt tgattatttt tagcttataa tatccaattt
     2221 cttttgtatc ataattttca tctattttaa taatacaagg gcttcctact gaatttgatg
     2281 ttttcgaagt agtcgaactt gaacttacaa tttcaccgtt ggaaaacttc gcgtaagatg
     2341 ttgttccatt tttataataa attgtaacat tgccatcaag ttctccattt tttactggcc
     2401 cttttataat atcaccattt ggcatagtta taactacact ttcagaaata acgccttcga
     2461 tatagtaacc ttcatattta cttccattac tataagtaaa ggcaccgaaa cctgtcaaac
     2521 ttccttcttt taattctcct tcataaattg ttccatctgt aaatgtcatt ttcccttgtc
     2581 catttttttc accattttcc cagttacctt catagatatt cccatcttca ccataatatg
     2641 ctccttgtcc attgggcaaa tcattagaga aattgccaat gtatctagcc acttcgacac
     2701 ctcccttctt aaaatacagt cgcccttttc catcaaattt atcattctta aagctaccat
     2761 aataatgtaa atctggcagg ttcaactctc caagtccgtc ttttttattg aatttccaat
     2821 caccttcata gaagtattct acattttcta atataaatat ttccctaccc attccgtgtt
     2881 ttttaccatg ttgcatatac cccatatatc cacaaatagg cttcccatct aaagttgatg
     2941 ttataaaact acaccaccca tctgcatacc cttctttgca ctccatttct tttataaaat
     3001 atgatctgtt ttcacaattt atatcgcacc atactgtgca ttttccccct gaagctaatt
     3061 caacagagat atatttccca tcctcagaat catttattgc caaatcggca tactccttat
     3121 catctaacca ttctcctgtg tattttttac catttgcata ccaaattgtt ccataaccac
     3181 ttcttatatc attaacccaa tcaccttcat atttatctct attagcatat atcatggttc
     3241 ctttgccttc ccttgtgtct ttttcccaat taccatcata actactgcca tcagcataag
     3301 tcctttttcc cttaccgtga gcaactcctt tactaaattc gccaacatat ttttgtccat
     3361 ctggccaagt gtatgtacct tgcccagtga cataatcatc tacccaattc ccctcatata
     3421 tctgttttga ttgattgtaa gaatgtacgc caaacccatt acgcatatca cttttccaat
     3481 ccccaacata cttatctcca tttgcataaa tcatagtacc gttaccttct ctaatgcctt
     3541 ttttccatgt accttcatat gtgctaccat cagaataagt tcttattcct tgtccatcaa
     3601 atgttccgtt attaaattcg ccaacatatt tttgtccatc tggccaagta tatgttccta
     3661 gtccattagc tatttgatca tctatccaat tcccttcaaa tgtctgattt gatttattat
     3721 atttatgcaa tccaaatcca ttgcgtctat tatcaagcca ttccccagta taaacatctc
     3781 cattagcata attcattacg cctttccctg atttcttacc gtctttccat tcgccatcat
     3841 acgcttctcc atttggataa ataattttac ccaaaccatc atatctattc gattttaatt
     3901 ctccattgta gaacgtgcca tctttgtagg taattttccc ttctattcca tagtcatcat
     3961 taatccaatt accttcatac ttatcaccac ttgcatataa catggttcct tgcccatgcc
     4021 ttttgtcatt catccattcc ccatcatata ttccgccatc tttgtaggtc attttccctt
     4081 gtccgtttct taaattttgt ttccataagc catcatattt gccttggtta tcaaacatta
     4141 atcccctccc acttcgttca tcatcaatcc aaaaaccgtc atattttcct tgagtgctaa
     4201 aaaaaatctg tattccgtac ccattccttc tatttaccgc ccaattacca gtatatctat
     4261 ttccaactga atcaagaaat tggccattgc cttggcgttt attatctaaa aaatatccaa
     4321 cataggttgc tccgttatcg taaaagattt taccataacc attggcttta ccattgattt
     4381 cctgaccgaa gtattttcca caattcccat caacgcatat tttatctcga aaaaacactt
     4441 tataaatata aacatcctta tcatatacac aggcaatatg catactatca gggctaaaac
     4501 gcacgtattt taatgattta ggaattgttt taatacaatc tccgctattt acaacatata
     4561 ttttagtttc ttttgaacca ctactaacaa tggaattgcc atcagggcta aaacaaatac
     4621 tattaatata atcctcggtt tcattattta tattattatc ggcaatagtc ttttcgctta
     4681 atgtaataat cgggttatta ttaatatctc tgattttaat attgttattt cgatccacat
     4741 atgcaacata atctccatta gaactaatgt ccaatgttcc cgcatatcta ttagaatcat
     4801 caatattaaa cgcctgagag aagttgttat ttaccctctt cattataata atataattcc
     4861 catctgtgta atatgcaaaa aaacgaccat cacggcataa atcaattatt ttgtccgaaa
     4921 actcagatat ttcaataatt ttttttatgg caacatcatt tttaatgtcc cataatataa
     4981 tactattact tgatctgctt aaaattgaat tgccatcatt actaaaaaca attgagtaaa
     5041 caggtttctt tttgtgacga tataaattct tagtgttttt ttcttcttga agatcccata
     5101 actttacagt acgactgttc gatgcacttg ctatgaattt accatcaggg ctaaaactaa
     5161 ctgagttaac agctttaaga ttatgttttc ttaaagtttt aatacatttt ccagaattta
     5221 catcccaaat tcttatggtt ttatcatttg atccgcttgc taataatttc ccatcaggac
     5281 tatagcaaat cgtattaaca atatcagtat gaccacttaa tacggtttca gttatttgct
     5341 cttgagagaa acaaatctta ccgattacag tcaacaatat tataagcttg atagaaatta
     5401 ctctcttttt aaaataatat tccttcatac tatctgtaag tttataatta gaatgatcta
     5461 atagctctaa catggtaagc aaatgttcta ttactccagt tcatttgtcc gccgctgtag
     5521 aagtcctgaa accatgcatt gtcgatatca tcttcatttg aactccaata atatccactt
     5581 gtaaatcctc caacattatc tttttgcaaa aataataagt ttaactcata tttagaagga
     5641 agataccaat ctccgtaata ctcattattt aatgtaacag cataatcatc acacacttga
     5701 gctgcataat ttccaactcc ttgattattt attattctcc ttgtgttttc ctttcctgca
     5761 tatacagcat ttaaagaagc ttctgtaact aggttttctc cattatacca ttgtattcct
     5821 gcactttgat catttattgc tgcaattaat ccgtgttgtc cggattcatc taaccaaaaa
     5881 ataattcctc cttgatagtt ctgtcctatt gtgagaacat tattaccgga tgaacctgtt
     5941 gcacctgttg cacctgttgc acccgttgca cccgttgcac ctgtagcacc ggtagcccca
     6001 gtaggaccaa tcggacctat ggcgcctgtt gtaccggtag caccggtagc acctgtagca
     6061 ccggtagcac ctgtagaacc tgtggcacct gtggcaccag tagaacccgt tgcaccggta
     6121 gggccaacgg gaccttgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     6241 caccagtaga acccgttgca ccggtagggc caacgggacc ttgcggacca gttgtaccag
     6301 ttaaacctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt agcacctgta
     6421 gcacctgtag cacctgtagc acctgtagca cctgtagcac ctgtagcacc tgtggcacca
     6481 gtagaacccg ttgcaccggt agggccaacg ggaccttgcg gaccagttgc gccatcagca
     6541 ccaacaggac cttgcggacc tgaaattgaa ctaccagaag attctgcata aagagcatat
     6601 ggcacgctaa ttaattgaaa tgtacctgta atactataac cgtttgaacc ctgaggatca
     6661 atttctgttt gtaaccaata caaacctgcg gaccaatcaa tgtcttcaaa aacaccagag
     6721 actaccactc catttccaat ttcaattgta acagaactgt tcccgtttgt tattggcgta
     6781 tgtgtttcaa tataaactgg ttctccatca gcagaaccct gcaatatcga aagtctcatt
     6841 ccaaccgcat tattaggaat aagttgacct tgttcatttc gaattattga ctgataactc
     6901 attttttgag gaacctgagc ataaattatt gaggtcagca atattgcaat tgaaaaagta
     6961 agtagatttt tcataaaata ttagtgtttg attattgtaa aagttttcag agattgattt
     7021 aatgtaacta aatttaaaac atacgtacct gggtctaaaa gcttcatatc aattttcgtt
     7081 tcgccttcta ttacaactcc attataaagc attttcccat taatatcaaa taattgataa
     7141 tcaatattca agttttcctt atccccgatt tttagaatta aataatcgct tgtcggattt
     7201 ggaaaagttg caattgaaag attaacatta tcaaattctg aaatcccggt ttcccatatt
     7261 tcgtatggtt gttgtacgcc catcgatacc gaaccagttt cgcttgatat atatgtgtag
     7321 gcaacctgcc caactgaaaa acttgtataa ccgcctgcac cctcactatt tccgcctaca
     7381 gaagtaatag catattgtgc atgtaaaact gaaggtaaag caaaaataag tagtaaaact
     7441 aattttctca taaacttatc gtcttaaatt tatcaataaa cacgtagctc cacaaactta
     7501 cccccaattt cccaaatatc caagacaaat ccaacatctg agatagattt ttcataggca
     7561 gttgttttgc acctgtttct caagcgtttt tgcacccgtg tcggatttca aaatgtgttg
     7621 gtctgtgctg ctgagtccgg tcggtatgtg tctgcatagc cgggaaaccg aactgcccat
     7681 cacaacattt gacttatagc tttttcgagg gcatatattc atccgagtcc cgaagcggtg
     7741 gctcggttgt ggaatggaaa ttacatcatt gttcaattca aaaaaaatca ctatttttca
     7801 aaacattttg gtaagttttt tattatatat ttgaatcaaa atgcattact atgaaaaaat
     7861 ttgtatccat tctaatgttg ttatcgcccg tgttgttgtt tgcgcaagac tacccggttg
     7921 gttaccgcga aatttctgtt tatgattcaa accgtgaaag agatattact gcctatgcat
     7981 attatcctgc agttcaggca gaagaaaatg cctcgcctgt cgaagggacc tttccttata
     8041 tagtatttgg acacggattt atgatgaatc aaggcgatta cgattatttg tacaactacc
     8101 ttgtaccgaa aggttacttt ttcgtatcgc tcacaactga aacaggtgct tcacccgacc
     8161 acgaaaccta tgcacaggac cttgcttttg tttgcgataa tttttacgat ttgatgcttg
     8221 acgatgttgt ttttcacgat tgcgttggtc ccaaatgtgt tgttatgggc catagcatgg
     8281 gcggaggtgc ttctcatctg gcttcgtcat atacccagaa tatggaagcc atcatcacct
     8341 ttgcggctgc tgagaccgat ccatcagcca ttgatgcggc tgcaaacgcc aacgtaccca
     8401 ccattgtatt ttacggcgaa ggcgatgctg ttgcaccacc cgacgaaaac cagattccca
     8461 tttataacag ttccaattcc gattgtaaaa tcctgatcgg gatcaatggg ggtattcact
     8521 gctattttgc cgaatcgcaa tgggcatgcg attttggaga aaccatgtcg ggttccgatg
     8581 ccggaattga acgcgaagag cagcatgaca ttgttaccga tttgctcgga ttgtatctcg
     8641 acaaaaaact gaagggcaat cacgcttcat ttctgcaatt tgttgattca gtcgataatt
     8701 catctcggat aaccactcag gccagttgtg attttacgga gattacaact gacgagttgt
     8761 atcaatttga attctatccc aatccaactc aagatgtcat agaaattaac attgcttcta
     8821 ttgatttcga ggtaagcatt atcaatatca tggggcaaac cgtaaaggag gtgcacaata
     8881 aaaagctaat cgatgttagc gatttaaaac cgggaactta tgtaattcag gttataacgg
     8941 gttctcgaag catgcgaaat aaagtaatca tctggtaatt atattttaca aaacaggaaa
     9001 tttgtaaata ctacttcata ctaatacaaa atattaacta atctattcga ataaataact
     9061 ctatctgaga ttgtcaaaaa tcgactttta tatatttttg cctatgtaat tgagtcgacc
     9121 aacaaagatt aaaaaatgca ggcaattcgt attcggcaaa taagctttat aataggctta
     9181 ataataattg caaaatcagg attttctcaa acagatatac ccgatagttt taattcttat
     9241 attgattctc ttaccgaatt aagccttaat tctcctaatc cggaggttcg tgtcgatgca
     9301 ctcaacaaaa tttcgactga atatgcacat cgaaatcctg taaggatata caaatatgcc
     9361 attgatgcaa tagaattggc caatgaaatc aactatacca ccggcttaat taaagcttat
     9421 tatcatctgg gggattatta ctatttccag gcaaatttcg aagaagcaca aaaatatttt
     9481 tggaaatcat attccatcgc taatcaacac aatatagatt ctttagctgt agatgctctg
     9541 aatgtgattg ctgttattaa tgccagcatg gggaattatg acaaagccat aggatatttc
     9601 gaaaaaatca taagaataag tcaggaaaaa caattttcaa gctccgaaaa tcacgcaatg
     9661 attaatattg gcaattgtta tcgagaacaa ggtaaaaatg aattggcaga gaaatattat
     9721 atcagagcct atcaacaagc aaaaatcaga caggctaacg atgaacaaat ctattcgtct
     9781 cataatctgg ctgtttttta cggcatgacc ggaaattatc cgaaagcgat tatgtattgc
     9841 aacgaagcca ttgctttggc cgaaaaaatt caaatgaacg agcagatact cgaaggacaa
     9901 aacatccttg caaacatcta tatatatcaa aatcaacctg gtaaggcaat agagatcgct
     9961 gagaaggctc ttacaagagc cgacagcacc catgccaact tacaaaaaat gcatctctac
    10021 aacaaattga tgactgccaa tgagcagatt ggtgattacg aatctgcact aaaatggatg
    10081 aaactggaac aaaccatcaa agacagcatt ttcaacattg aaaaacattt ccagattaca
    10141 aaccttatgg aacaatacga atcaaattta aaagatctgg aaattgaaaa gaaaaacaat
    10201 gaactgggga gaaaaaactc gctgattcgt tttattttaa taatacttct tattgtggct
    10261 ctatcggcaa ccactatcat cattatgttt atcaggaaac gtaaaaccaa taaaattttg
    10321 gttaaaatca atcatgaact atcacaaaaa tatcaaaact ataacattga attcattcca
    10381 gctatttctg cttcagaatt aacctctaac ggtaaatcta atttcgaaga aaacaaaaat
    10441 gaaaacaatg taaaatatgt ttcctcaagc ctttcggacg aaagaaaacg cgaattgcta
    10501 aatcgattga acaaattgat gagcgaagat gctttatatc ttgatccaga aatcagtatc
    10561 gaaagagtga gcgaatcgct tcaggtgaat tccaaatatt tgtcgcaagt aatcaacgag
    10621 ggtctcgggc gcaatttccc acaatacatc aatgaattaa gagttctgga atcaatccgg
    10681 atactttccg accccaaaca ggacaaatac agcatcgaag ccgtagccaa tatgtcgggc
    10741 ttcaaatcaa aatcggtttt caatacagcc tttaaaaaac agaccggaat aacgccatcc
    10801 ttcttcagag cagagtcaaa aaacataagt attaattaat caaatactta cacacgttct
    10861 gttttccgaa tttcgaacat tataagcttt gcagtcaaaa ttcaaataaa tagctttgtt
    10921 taaatcaata ttaacaaacg ctatgaaaag aagagtatta ttattatcga tggtgggatt
    10981 gcttatactg ttttcggcac aagctcaaat aagtttggtt aaagacatcg caaatgctcc
    11041 agttaccggc aattcgtatc caaaagaatt tgtagagtta aatggcaaca ttctttttgt
    11101 tgcgaccgat ccaattcatg gtagcgaatt atggcgaagt gatggcacaa cagcagggac
    11161 atatttgctt aaagacatta atccggggtc gaacggtagt acgccacaat atttcactaa
    11221 atcaggtaat aaggtctatt tcaaagccaa taacggctct catagcaatg agctttgggt
    11281 tagcgacggt acacccgatg gtacattgat gttgaaggat ataaatccgg gtatagatga
    11341 gtcatatccc ggaaactttg ttgatatgaa tggaatttta atttttaaag cctatacgcc
    11401 cgaatatggc tccgagttat ggaaaaccga tggaactccc ggcggaacag tgatgataaa
    11461 agacataaat ccagggacag tgtccagcta tccaggaccg ggtatctgtg taaacaatgt
    11521 agtatatttt gcagcggaag acaccattca cggacaagag ctttggcgaa gcgatggaac
    11581 tgaagccgga acatatttgc tgaaagattg tgctatcgga aaacgcaatg gcactcccca
    11641 tgcttatgtc aacttgaata accaactgat atttgttgca tttgacacca tccatggatt
    11701 cgaattatgg aaaagtgatg ggacaactgc aggcacattt atgtattgcg atatcaaccc
    11761 cggatactgc gatacaactt atgatgatat accttctcag ttaattgttt ataacaacaa
    11821 agtgtatttc cagtctgcat tcgaatcgta tggacaagaa ctatttgtaa gtgatggcac
    11881 ggaagcaggt acttatatgc ttaaggatat tgatcctgcc gatgccagcc gaccgggtaa
    11941 ttttgcaata gccaataatc tattattctt tactgccgat gtgaatggat atggtaggga
    12001 gttatttaaa accaatggta cagcggccgg tacggtcatg atcaaagaca tttggcctgg
    12061 aaatacgagt ggttatccat tggcgctcac tgctatggga aattatttat tttttgccgc
    12121 caacgacagc ctgcacggtg aagaaatttg gaggagtaat ggcacaacag ccggtactgt
    12181 aatggtaaaa gacatttatc cgggcacaca gggtagtacc ccttctactt atgggatggg
    12241 aagttcaggc gaaaaggttt tcttttgtgc aactgatgga actattgttg gaagagaact
    12301 atatgccact gatggcacta tggccggtac tactctcatt aaagacattt attcaggatc
    12361 agcaggcagc gaaccccttt acctcacagc acttaatgga aatgttttct ttacagcaat
    12421 tgtagacact tatggtcgag aactattcat cagtgatgga actacttccg ggactcaact
    12481 gcttaaagac attcgtgtag gaagcagttc atcgtctcca ctcaatctta tcactttcgg
    12541 caacaaacta ttgtttacag caaacaatgg gtcttcgggc acagaattat ggtcaagtga
    12601 tggcacaaca gccaatacaa gtatgataaa agatatatgc ccatgggcag gcagctctcc
    12661 tcaaaatttc atccaaatgg gtaactatgt atatttcgat gccaatgact gtaacaatgg
    12721 aactgaattg tggaaaaccg acggaacaac agcaggtaca acaatggtca aagacatcac
    12781 tcccggaagc aatgggtctt cttttggcac taagttctta cacaacaatc atatttattt
    12841 taatgtctgg tcgaatagca ttggtttcga gccttgggct agtgatggaa ccgagattgg
    12901 aactttcctg ctaaaagaca ttgctcccgg caatcgtagt ggttcgccat caaaacattg
    12961 taacctcgac acactgatgt tgttcaatgc atacgactca attcatgcca acgaactttg
    13021 gataagcgat ggtactgaag cgggcactca ccttttaaaa gatatcaata ccggagaaaa
    13081 tagctcgaat ccaaataatc taacatcact cgatagttta attgttttca ctgcctttca
    13141 acccgaaaca ggcaccgagt tatgggttac caacgggaca tcagatggaa cccatttatt
    13201 aaaagacttt tctacaggtc catcaagttc taacttaagt aatttcttcg cattcaataa
    13261 ccaaatattc ttttcagcgt ttcaagctgc tacgggctat gaattgtggg ttacagatgg
    13321 tagtattcaa ggtacatata tggtaaaaga tataaatccc ggagattatt catcgaatcc
    13381 ctccgatttt gttgtaatcg ataacaagtt gtacttttat gcaaacgatg gaagtcatgg
    13441 cattgaagtt tggacatcgg atggctccga agtgggaagc tttctcgtat ctgaaggaat
    13501 cgaaggcatc gataatctaa atgccagaaa aatggttgca aacggttccc atgcattatt
    13561 ctgtgctcaa aatagtaact ctggttatga actgtggcgt ttcgacccac actatgtttc
    13621 agtgactaac gtaaacacag atagtgtttg tgtcggtgct gaaatatctg taatattcga
    13681 agctgttggc acatacaata gcgggaactc ctttgtttta caaatgtctg actcgaatgg
    13741 aaactttgac aacccaacaa tcattaactc cataagtgcc ttggaatctg acaccattat
    13801 cggaaatatt cccggtacta tagatggagg aacatatgta ttcagggtag tcgccacatc
    13861 acctgaaact aaccgtggag ccattagtca gtcagtggtt atcaatcctc ttccccaaat
    13921 aagtattgac aatgttagtc ctatttgcat aaatgctact cccattgaac taagccatgg
    13981 acagcctagt ggcggaagct attcaggagt aggtgttagt agcaactttt tcaacccagc
    14041 tctatgtggt tcaggagaga ccagtattac gtatagcctg actcaaaact cttgtaccaa
    14101 tacagtaact tattccatta cagtgagccc acttccggct gccaaccaca gtatcaccgg
    14161 agagcaaagc atctgtcagg gacaacaaaa tgttgtttac caaattgagg ctctcgacaa
    14221 caccgattcc tatgcgtgga cattgcccga cggtgcaaca ggaaacagca ataccaacag
    14281 catcagcgtt aactttgggc tgaatgctat gtcgggaaac atcagcgtta ccgggcacaa
    14341 caattgcggc gatggtcaaa ccattagttt gcccattacg gtaaacccac ttccggctgc
    14401 caaccacagc attaccggag aacaaagcat ctgtcaggga caacaaaatg ttatgtacca
    14461 gattgatgct ctcgacaaca ccgattccta tatctggacc ttgcccgatg gtgcatcagg
    14521 aaacagcaat accaacagca tcagcgttaa ctttggcctg aatgctatgt cgggaaacat
    14581 cagcgttaca gggcacaaca actgcggcga tggtcaaacc attagtttgc ccatttcgat
    14641 aaacccactt ccggctgcca accacagcat taccggagaa caaagcatct gtcagggaca
    14701 acaaaatgtt gtttaccaaa ttgaggctct cgacaacacc gattcctatg cgtggacatt
    14761 gcccgacggt gcaacaggaa acagcaatac caacagcatc agcgttaact ttgggctgaa
    14821 tgctatgtcg ggaaacatca gcgttaccgg gcacaacaat tgcggcgatg gtcaaaccat
    14881 tagtttgccc attacggtaa acccacttcc ggctgccaac cacagcatta ccggagaaca
    14941 aagcatctgt ca
//
LOCUS       DAOOQP010000073        14927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4899_length_14927_cov_5.633136, whole genome shotgun sequence.
ACCESSION   DAOOQP010000073 DAOOQP010000000
VERSION     DAOOQP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4899_length_14927_cov_5.633136"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLK75_08380"
     CDS             complement(<1..369)
                     /locus_tag="PLK75_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39302.1"
                     /translation="MPNNSIFLIVIIAYFTFLILWGLYQGRKVKNAGDYAIAGRKLPG
                     WVAALSERATGESSWALLGLPGAAYATGVMEIWTAVGCVAGIVTSWAVLSWRLRKESE
                     KYQVSSFVDYLSKKHGDYAKP"
     gene            432..1706
                     /locus_tag="PLK75_08385"
     CDS             432..1706
                     /locus_tag="PLK75_08385"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOY39303.1"
                     /translation="MKHILILLLAITQYTISFSQQIRYSEYFDTAALRVDYIHSGTKN
                     TEAVSFVRFVRDDRWAGNPDKLICEFEYGKYRVEVFDAVSNKLIYSYGYCSLFEEWQT
                     TDEATLTNKAFGESIVIPFPLKAVRIELFSVQRDNSKTKIFTFNFNQSTEFELVGNHA
                     QVNTLLINGSVEHSIDVAVVADGYSLNERGKFESEADFLVKYLLSDPAFKDSNKKFNI
                     HLVFSPSLESGCDIPGKGLWLNTVADSRFYTFGSDRYLTTASYHKLRDLVSKVPCDQI
                     IIIANSNIYGGGGIYNFYSIASGSNLQSAEVLVHEFGHAFAALGDEYYTSETSYNDFY
                     DLNHEPYQPNLTTLVDFDSKWKNMIDENVPIPTPLDVKFKNKTGVFEGGGYVEKGIYR
                     PAFNCKMKELSSPFCKVCSRAIMKMIDYYCEP"
     gene            complement(1783..3345)
                     /locus_tag="PLK75_08390"
     CDS             complement(1783..3345)
                     /locus_tag="PLK75_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOY39304.1"
                     /translation="MKKTVSINLNGLIFNIDEDAFMVLSDYLEKLKAYFSKKEEGEEI
                     MSDIELRIAEIFTSKISPSKLVINISDVEEVMNTMGRVEEICDGDSSTDTGSRAGEKK
                     NQKKFFRDTDNRLLGGVCAGLSHYSGINLLAIRILFVVFFLFSAGFVTLVYFILWLIV
                     PKAVTVADRLEMKGKDINISNIENTIKEEYKEVKENFRKMKNSKNFQDNLNRSGRVMA
                     GSLNIIFGIFGKVLGALFIIIGVFFIFVLGLGLFASSSEFFINEIAGLEIVPLPYILS
                     LFTNPTISWLFVVALSLVCFIPLIAIVYAGINIVLKLKSVKILNLSMFILWLVGLSML
                     IALSISMSSHFVSSNDVVLKNIVIADSAKTIHVNTLSNMGEDDFMEHVFIENLRLVKV
                     NNAVKAAGQVEVSVHASTDSIAYFEVQKCSRGENRSQAGKNASMIRYRFAVQDSLITL
                     DEYFFLPDGFKLRGQEVEIGICIPVGTKLVLDKNTMPLIEDTESVGNIDFDELQSGTF
                     LMTPQGLILVAE"
     gene            complement(3347..3682)
                     /locus_tag="PLK75_08395"
     CDS             complement(3347..3682)
                     /locus_tag="PLK75_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOY39305.1"
                     /translation="MESEKTNAQMRKGILDYCILLIISRKDAYVPDVISLLKDSGLHV
                     VEGTLYPLLMRLKNNGCLDYRWEESSSGPPRKYYKITEMGLQILQELDRDWHELEESI
                     HYLKNYKPE"
     gene            3905..5098
                     /locus_tag="PLK75_08400"
     CDS             3905..5098
                     /locus_tag="PLK75_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HOY39306.1"
                     /translation="MKRKITLIALLSAALLIGVTSCSKKTANLNFVPKNAFVVMTVDG
                     KSWKDMVKPEKMLENDQYKEGMKEIEQTSKKLAEFIEKVAKDPNELGIEADGMMYGFV
                     SMEDENLLLGFIAGVDKKKFEENLKMISEELDFELETKEKDGITYFMPEDELILGWDK
                     NKVMLLGLVDGHDFDIEKELFALMNQKKSESILDDKDFNAFLKDSKDFNIWISSNIVN
                     VVDDNEEIAELEKMLGIDFNDNYGHIFFEYTKGESTMTAKLRFNESIQKADWNKVFKN
                     LTEMGMLDGIFSEFGGFGGSAAEADVKLMFMVMDDYTMAAREAIADKVISQEEADQLN
                     TLMEELNDYSDEFEVKYKDDAKAAKLFEELSKEPEYEKILTELMQVSFELYGCKGSEL
                     LEMGE"
     gene            5134..6120
                     /locus_tag="PLK75_08405"
     CDS             5134..6120
                     /locus_tag="PLK75_08405"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HOY39307.1"
                     /translation="MKKNIQIVLIALVGGALFLSSCTKKSDCLTNIPKGAFVVVSIDG
                     NACHEMMDPNILLENEQFKEGMTQIEETSKKIADLINKSVKNPDETGIKLTEQVFGFG
                     EAEKENLIIGIIAGVDKAKFEENMKMIATEMKFEFATKEKDGITYFMPEETMIMGWNK
                     NKIMILTLAEGYDYDLEKKLFSLMNQKKDESILENKDFNIWLKGCKDFNVWATTNVID
                     QIFNAKDIAGYESSIGLDFNDNYLHIFFDAEKAEWTLVTKIRVNESIQNADWNKVLKN
                     LENLGIMEDLMNSIWGGGYDDEYEDYDWESDTAYEALTEEEWAEIEELTKTN"
     gene            6147..6788
                     /locus_tag="PLK75_08410"
     CDS             6147..6788
                     /locus_tag="PLK75_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY39308.1"
                     /translation="MNLSFSKLIPSPFLDTDYSVSQVWGRDFTLQGSTSVLVSAASGK
                     GKTTLVNIIIGQRNDYVGTMLIDGKSPLDMNARELSILLSRKISTVHQGLMLFDELDT
                     WQNIALKNRITNFKTDSEIDEMLNQLGLAAFKKQKIKRLSYGQKQRVAIIRALCQPFG
                     FILLDEPFSHLDSENASKAWDLIVSEAKLQQAGIVMTSLGGDHKISFDQILQL"
     gene            6785..7990
                     /locus_tag="PLK75_08415"
     CDS             6785..7990
                     /locus_tag="PLK75_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY39309.1"
                     /translation="MIWKVLKTEISATQLSAAFMGAFAGLVIMLAAAGFYVDVIGVFD
                     DNEGFWKEEYIIISKRITSDETLRQINEDVKNKPIFSKQEIDELCSKSFVKQVTPFSN
                     CTFGVAAFTGKEGPMAGFYTDMFFEAVPREYIDVNYEGWSWKEGDSFIPVIIPKTYLN
                     LYNFGFATTQNLPQVSEKSASLVKFNVLITGKNQRKKFEARIIGFSDRINTILVPKEF
                     IEWGNKNFGDSEIPDPNRVIVVANDPSNPEMFRYFEKQGFDVNKSELSNSKALMFLRI
                     IISMVISVGLLITALAFWLMITSIMLLLQKNKNNILKLKLLGYHPEKISRPYYLLSLV
                     MIGAIALLSVIPLYFIRSFYTGKMLAMGYESAATYTPILALLMLSFVIALLLICLISI
                     RIRVRQFIK"
     gene            8105..10462
                     /gene="feoB"
                     /locus_tag="PLK75_08420"
     CDS             8105..10462
                     /gene="feoB"
                     /locus_tag="PLK75_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOY39310.1"
                     /translation="MLLSEAHVGESYVVARLSGRWGIRRKLIAQGLEHGVTIRIKSID
                     SENFHVAVNGHIGRRRLPLTLAPFVTLVSANDKNLHEKLDDLCNSIPVISFGNPEILN
                     SVFGTDIADFRESIVFQHDHEIHITHLPNTDTIAGNGENETKIRELITQEYPDLIVGI
                     IDASRLKEDLYLASFFIDMDVKMMLIITGLEHLDEQGLKLDYEHLGKMLGIPILPYKK
                     PEDKPLILRTLLNIHHNTEPYVRSIDINYGDELERSIRRIEKHIVKDMALHDEIAPRY
                     TALRLLENDENTQKNVSYCNRCTRRSCLTGSEIKRLEKLFDLPVMQIVHEARMSFING
                     GLKENLTHGKSKKAVKTEKRLDAILTHKIWGIPIFFGFILLTFFATFQLGKYPSDWLE
                     KGILMLMDYLGKTMHEGALKSLLIDGVLGGVGGILIYIPNIFILFFFIGFLEITGYLS
                     RGAFLVDKYMHHIGLHGKSFIPLIMGFGCTVPAIMATRMLDDRRNRLLTMLIVPFMSC
                     SARLPVYVLMIAAFFPEHPTLILFIIYSIGILFAIITAFLFNKTIFRKKEVPYVMQLT
                     NYHKPSLKMLTHYTWIRGREYLKKIAGIILLASVLIWALGYYPRDIKYSKNYDTLVTE
                     ATDTKEVKKLNLAREAEQQENSYIGKIGKFIQPAMEPLGFDWKMSISVLTGIAGKEIV
                     VSTMGVLYQASDENGHSAESLPVKLSSEKHTSGNNAGKPVFNKVSAFAFIIFILLYFP
                     CMGVVVAIWKESEKIKWSVFTVIYTTALAWIMAFLVNQIGSALFL"
     gene            complement(10813..11127)
                     /locus_tag="PLK75_08425"
     CDS             complement(10813..11127)
                     /locus_tag="PLK75_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOY39311.1"
                     /translation="MKGFMYILKCANGKFYTGSTIDLEQRVNQHKSGTGSNYTRKHLP
                     VELVYFEEFQYIDQAFYREKQVQNWSHKKKEALIIGSVEKLPELSKCKNTSNYKESKK
                     KL"
     gene            complement(11680..13119)
                     /locus_tag="PLK75_08430"
     CDS             complement(11680..13119)
                     /locus_tag="PLK75_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOY39312.1"
                     /translation="MEPYILVIVSAGVLFLFILLITFLRRYKRCPSDRILVVYGKVGK
                     KGTDESRSSRCIHGGASFIWPVIQDYQFLDLTPISIEINLKSALSKQNIRVDVPSIFT
                     VGISTEVGIMSNAAERLLGLAREDISNLAKDIIFGQLRLVVATMDIEEINSNRDKFLA
                     NVSTNVEQELKKIGLKLINVNVTDIKDESGYIEALGKEAAAQAINEAKKSVAEKNRDG
                     SIGEANAMKDQRIQVSQANATAVDGENMAAIAIANSSSNRREKEADAERKAVAAEKVN
                     KARALEESYSAEKSAEIIRAQREEATRQADIIVPTKIDKEKIEIAAEAIAEQTRRHAK
                     GEADAIFMKMEAEGKGIYEILTKQADGFEKLVKAAGSDPQKAVMLMIADKLPDMVKTQ
                     VEAIKNIKIDKITVWDGMSSGKDGKSPATANFLSGMLGSIPPMQDLFKMAGMELPEYL
                     KGKVQTDQYSNVKAEEIPPAEPPKTDKKK"
     gene            complement(13138..13725)
                     /locus_tag="PLK75_08435"
     CDS             complement(13138..13725)
                     /locus_tag="PLK75_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39313.1"
                     /translation="MLLSFENWWQAMELAEKIYWCIAVPFSVVFIIQLIMTLIGGDFE
                     AGASTGDADVAVDSDAGIGFQFISIKNIVGFFTIFGWTGIACSVGGLSVILSLVIALA
                     AGLLMMLIMALLIYYLSKLAEDGSMKMSNAIGKTATVYLPIPGKRAAQGKVQLTLHGF
                     QTLDAITDDEEAIPNGTLVKVTDIINNEILLVTRL"
     gene            13864..14526
                     /locus_tag="PLK75_08440"
     CDS             13864..14526
                     /locus_tag="PLK75_08440"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOY39314.1"
                     /translation="MKALVIGATGLTGSSLLEQLLSDTHFTEITVLARRSTQVVSEKV
                     TEHTVDFDKPESWKHLITGDVLFSALGTTLKLAGSKQAQYTVDYHYQLAVAKAAAENG
                     VGRIVLISSYGADAKSGVFYLKMKGELEESIMQLGFKNCVMLRPGQIDGKHSPQRAGE
                     KFALKAVKALNRCSILKRYTPITPQKLATAMINAALLNEEKNITIVELEQVHKLAEKQ
                     IV"
     gene            14523..>14927
                     /locus_tag="PLK75_08445"
     CDS             14523..>14927
                     /locus_tag="PLK75_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39315.1"
                     /translation="MRGNNGILVPIFIIILSLLTDWYVLQGLKTIILPIRQPGIRQAI
                     IILFRIFSIGVIVGTIIGGYFVMVKGEFTLMFRIFMHIFLIYFTAKIAFILFLSFEDI
                     VRLFRGTISLISSGTLGSFLPPRSFGVSVAGLS"
BASE COUNT         4581 a         3036 c         3008 g         4302 t
ORIGIN      
        1 aggttttgcg taatcgccat gctttttgct aagataatcg acaaaactgc taacctggta
       61 tttttccgac tctttacgaa gccgccacga aagtacagcc catgaagtta caatccctgc
      121 aacacaacca acagccgtcc agatttccat tacaccggtt gcatatgctg caccggggag
      181 ccccagtagg gcccaggagg attcccccgt cgcccgctcc gataaagcag caacccatcc
      241 gggtagctta cgcccagcaa tggcataatc acctgcgttc ttaacttttc ttccctgata
      301 aagaccccat agaatgagaa atgtaaaata tgcgataatg acaatgagaa atattgaatt
      361 attaggcata atgtttgatt tttgccaaat taaaaaattg tattattgta atgaaaaatt
      421 tgtgaataaa aatgaagcat atattaattc ttctgctagc cataactcag tacactattt
      481 cattctcgca acaaataaga tattcagagt atttcgatac tgctgcattg cgggtcgatt
      541 acattcacag cggtacaaaa aatactgaag cagtcagctt tgttcgtttt gttagagacg
      601 accgatgggc cggaaacccc gacaaactga tctgcgagtt tgaatatggc aagtaccggg
      661 tagaagtttt tgacgcagtg agcaacaaac ttatatacag ttatggatat tgcagtcttt
      721 tcgaagaatg gcaaactacc gatgaggcaa ctctaacaaa caaagctttt ggagaaagta
      781 ttgtaatacc cttcccccta aaagccgtgc ggatagagtt attctctgta caacgggata
      841 atagtaaaac aaagattttt acattcaatt ttaatcaaag cactgaattt gagcttgttg
      901 ggaatcatgc ccaggttaat accttactta taaacggcag cgttgaacac tctatcgatg
      961 tggctgttgt ggcagacggg tacagcttga atgagcgtgg aaaatttgaa agtgaagccg
     1021 attttctggt aaaatacctt ctgagcgatc cggcctttaa agacagcaac aagaaattca
     1081 acatacatct tgtttttagt ccttctttgg aaagcggttg cgatataccg ggtaaagggt
     1141 tatggctaaa caccgttgcc gacagccggt tttacacctt cggttccgac aggtatttaa
     1201 caaccgcttc atatcataaa ctgagagacc tggtaagcaa agttccctgc gaccagataa
     1261 ttattattgc aaacagcaat atttacggag gcgggggtat ttataatttc tacagcatag
     1321 catcgggaag taatctgcaa tcggctgaag tgcttgttca tgagtttggg catgcatttg
     1381 cagcccttgg agatgagtat tatacttcag aaacaagtta taatgatttc tacgatctga
     1441 atcatgaacc ttatcagcca aacctgacaa ctttggttga tttcgacagc aaatggaaga
     1501 atatgattga cgaaaatgtt cctattccaa cacctcttga tgtgaaattt aaaaataaaa
     1561 cgggagtttt cgaaggcggt ggttacgttg aaaaaggcat ttaccgcccc gcattcaact
     1621 gtaaaatgaa agagctttca tcgcctttct gtaaagtctg ttctcgtgct atcatgaaaa
     1681 tgattgatta ctactgtgaa ccctgaaaat tgaaaaacaa tataattacg gggagcaagt
     1741 ggaatgactt gctccccgta atacatattt tatgaatcat ttctactcgg caaccagaat
     1801 caagccctgg ggtgtcatca gaaaagttcc ggattgcaac tcatcaaaat cgatattacc
     1861 caccgattca gtatcttcta tcaatggcat tgtgttttta tcgagcacaa gttttgtacc
     1921 gacaggaata catatcccga tttcaacttc ctgtccgcgc aatttgaagc cgtcaggcaa
     1981 aaagaagtat tcatcaaggg taattagaga atcctgaact gcaaagcggt atcttatcat
     2041 tgatgcattt tttccagcct gactccggtt ttcgccgcga ctgcattttt gtacttcgaa
     2101 atacgcaata ctgtcagttg atgcatgaac cgagacttca acctgtccag ctgctttcac
     2161 cgcattattc accttaacca gacgcaagtt ttcaataaaa acatgctcca tgaagtcatc
     2221 ctcacccata ttggacaggg tgttgacatg gattgttttt gccgagtcag caattacaat
     2281 atttttcagc acaacatcgt tcgaacttac aaagtgcgat gacattgata ttgataatgc
     2341 aattagcatc gataaaccaa cgagccatag aataaacata ctcaggttaa gtatttttac
     2401 cgacttgagt ttgagaacaa tattaatacc ggcataaact attgcaatca aagggataaa
     2461 gcaaacaagc gataaagcca caacaaaaag ccacgaaatg gtcgggtttg taaatagact
     2521 caatatataa ggcaatggca ctatttcgag tcctgcaatc tcgttgataa aaaattcgga
     2581 actggatgca aatagcccca aaccaagtac aaaaatgaaa aatactccta taatgataaa
     2641 caaagctcct aaaaccttcc caaaaatacc gaatattata ttcaacgaac ccgccatcac
     2701 cctgccggaa cggttcaaat tatcctgaaa gttttttgag tttttcattt tcctgaaatt
     2761 ctccttcact tctttgtatt cctctttaat tgtattttcg atattcgata tgttaatatc
     2821 tttccctttc atttcgaggc ggtcggccac agtaaccgct ttgggtacaa tcaaccaaag
     2881 aataaaatat acgagggtta caaagcctgc cgagaataaa aagaaaacaa caaatagtat
     2941 tctaatggca agcaggttta ttccagaata atgcgacaga ccggcacaaa cacctccaag
     3001 aagtctgttg tcggtatctc tgaagaattt tttctgattt ttcttttcac ctgcacggga
     3061 gcctgtatct gttgaactgt ctccatcaca gatttcttct acccgcccca tcgtattcat
     3121 cacctcttca acatcgctga tattgattac aagttttgat ggcgatattt ttgatgtgaa
     3181 tatttctgcg atacgcaatt caatatcact cattatttct tcgccctctt cttttttcga
     3241 aaaataggct ttcaactttt caagataatc cgaaagtacc ataaaggcat cctcgtcaat
     3301 attgaaaatg agcccgttca aattaatact aactgtcttt ttcatgttat tcaggtttat
     3361 aattttttaa ataatgaata ctctcttcca gttcatgcca gtctctatcc aactcctgca
     3421 gtatctgtaa gcccatttca gtaattttgt aatattttct aggtgggccg gaactcgact
     3481 cttcccagcg ataatcgagg catccgttgt ttttcaggcg cataagcaat gggtacagtg
     3541 tgccttcaac cacatgtaac ccggagtcct ttaaaaggct tatcacatct ggcacataag
     3601 catccttcct gctgattatc agcaaaatac aataatcgag aatgcctttt cgcatttgtg
     3661 cattggtttt ttcgctttcc ataggtttat tattaatatt gtattgcaaa gatataatat
     3721 tttattaata ccttgtttta catagtagta tttttaacat tattttaaca atttatctgt
     3781 atctgttaaa aaaaaactca aattaatttg tggaaaaata atttactaag tatatttaaa
     3841 cattattact ttaatagtct aaatgcagtt taaccttata attatcaatt aaataccact
     3901 aactatgaaa agaaaaatca cattaatcgc cttactatct gctgctttgt taataggcgt
     3961 tacatcctgc tctaaaaaaa cagcaaatct aaactttgtt cctaaaaatg ctttcgtagt
     4021 gatgactgtc gacggtaaat cgtggaaaga tatggtaaaa cctgagaaga tgctcgaaaa
     4081 cgatcaatac aaagagggca tgaaggaaat cgaacaaacc agtaaaaagc ttgctgaatt
     4141 tattgagaaa gttgcaaaag atcccaacga gctgggcatc gaagccgatg gaatgatgta
     4201 tggttttgtt tcgatggaag acgaaaacct gcttcttggt tttattgccg gagttgacaa
     4261 gaaaaaattt gaagaaaatc ttaaaatgat ttccgaagaa cttgattttg aacttgaaac
     4321 caaggaaaaa gatggaatta cctattttat gcctgaagat gaattgattc tgggttggga
     4381 taaaaacaaa gtaatgctgc ttgggcttgt cgatgggcat gatttcgata ttgaaaagga
     4441 gctttttgct ttgatgaatc agaaaaaaag tgaatcgata cttgatgaca aagattttaa
     4501 cgcatttctt aaagacagca aagatttcaa tatctggatc agtagcaata ttgtgaatgt
     4561 tgtggacgat aatgaagaaa ttgctgagtt agaaaagatg ctgggtattg atttcaacga
     4621 caattatggc catattttct ttgaatacac caaaggtgaa agcaccatga ctgcaaagct
     4681 gcgcttcaat gagagcattc aaaaagccga ctggaacaaa gtgtttaaaa atcttaccga
     4741 aatgggaatg ctggatggca ttttcagtga attcggcggg tttggcggaa gtgctgctga
     4801 agcggacgta aaattaatgt ttatggtaat ggatgattac acaatggccg cacgtgaagc
     4861 aattgccgac aaagtaatta gtcaggaaga agccgatcag ctcaatacac ttatggagga
     4921 attaaatgat tattccgacg aatttgaagt gaaatataag gatgatgcaa aagctgctaa
     4981 gttgtttgaa gaattatcaa aagaacccga atacgaaaaa attcttaccg aattaatgca
     5041 ggtttctttt gagttatatg gatgtaaagg ttctgaatta cttgaaatgg gcgaatagta
     5101 agcaacaatt tttaaattaa aaataaaaat aatatgaaaa agaatattca aatagtatta
     5161 atagcactcg tcggtggtgc gttgttcttg tcgtcgtgta caaaaaaatc ggattgcctg
     5221 accaatattc ctaaaggtgc ttttgtagta gtaagcatcg atggtaatgc ctgccacgaa
     5281 atgatggatc cgaacattct tcttgagaac gaacaattca aggaaggaat gactcaaatt
     5341 gaggaaacaa gcaagaaaat cgccgatctt atcaacaaat cggtgaaaaa ccccgatgaa
     5401 acaggaatta aactaaccga acaggtattt ggatttggcg aagccgaaaa agagaatctg
     5461 ataattggga ttattgcagg tgtggataaa gccaaatttg aagaaaacat gaaaatgatt
     5521 gcaacggaaa tgaagttcga atttgcaaca aaagaaaaag acggaatcac ctatttcatg
     5581 cctgaggaga caatgattat gggatggaat aaaaacaaaa ttatgatcct tacccttgcc
     5641 gaaggctatg actacgacct cgaaaagaaa ttgttcagcc tgatgaacca gaaaaaagac
     5701 gagtcaattc tcgaaaacaa ggacttcaat atatggctga aagggtgcaa agacttcaat
     5761 gtttgggcta caaccaatgt aatcgatcag atatttaatg caaaagatat tgcaggatac
     5821 gaatcctcta ttggtctgga ttttaacgac aactacctgc atatattctt tgatgctgaa
     5881 aaggcagagt ggaccctggt tactaaaatc cgcgtaaacg aaagcataca aaatgccgac
     5941 tggaacaaag tactcaaaaa cctcgagaac ctcgggatca tggaggacct aatgaattcg
     6001 atatggggag gtgggtacga tgatgaatac gaagattacg attgggaatc agatacagca
     6061 tacgaggctc tgactgaaga agaatgggca gaaattgaag aacttacaaa aaccaattaa
     6121 tttttaaagg acatgtcctt tactgtatga acctgagttt ttcaaaacta atcccttccc
     6181 cgtttctcga caccgactac agtgtttcgc aggtctgggg aagggatttt actttgcagg
     6241 gatcgacaag cgtgcttgtt tcggctgcct ccggaaaagg gaaaaccaca ttggtaaata
     6301 taattatagg gcagcgaaat gactatgtgg gtacaatgct catcgacggt aaaagccctc
     6361 ttgatatgaa tgcacgcgaa ctcagcatat tactgtcaag aaagatttcg actgttcatc
     6421 agggcctgat gctatttgat gaactcgata catggcagaa catcgccctt aaaaacagaa
     6481 ttacaaattt caaaaccgat tccgaaatcg acgagatgct caaccaactc gggctagcgg
     6541 cctttaagaa acagaaaata aaaaggcttt cctatgggca aaagcaacgt gtagcaatta
     6601 tcagagcatt gtgccagccc tttggtttta tattactcga tgaacctttc agccatctcg
     6661 acagcgaaaa cgcttcgaaa gcatgggact taatcgtttc ggaagcgaaa ctgcagcagg
     6721 caggcattgt aatgaccagc ctcgggggtg atcataaaat cagtttcgac caaatattgc
     6781 agctatgatt tggaaagtac tcaaaaccga aatcagtgca acccagctca gcgccgcttt
     6841 tatgggagct tttgcgggct tggttataat gctggctgcc gcaggcttct atgtggacgt
     6901 aattggagtc ttcgacgaca acgaaggttt ctggaaagag gaatacatta tcatcagtaa
     6961 gcgtattacc agtgatgaaa cgctgcggca aatcaatgaa gatgttaaga acaaacctat
     7021 tttttcgaaa caggaaatcg atgagctatg ctccaagtca tttgtaaaac aagtcactcc
     7081 attttcgaac tgcacattcg gagtggctgc ctttactgga aaagaggggc caatggccgg
     7141 tttctacacc gatatgtttt tcgaagccgt tccccgcgaa tacatcgatg ttaactatga
     7201 aggatggtca tggaaagaag gtgatagttt tatacctgtt ataattccta agacatatct
     7261 gaacctgtac aattttggat ttgcaacaac ccaaaacctt ccacaggtat cggagaaatc
     7321 ggcatcattg gtgaaattca atgtattgat aaccggcaaa aatcaacgca agaaattcga
     7381 agcacgcatc atcggattca gcgacaggat taatacaatt ctggtcccta aagaattcat
     7441 agaatggggg aataaaaact tcggcgacag cgaaatcccc gacccaaacc gtgtaattgt
     7501 tgtagcaaac gatccttcaa accccgaaat gttccgctat ttcgaaaaac aaggcttcga
     7561 tgtaaataaa agtgagctca gcaatagcaa ggcactgatg tttctccgaa taataataag
     7621 catggtaatt tcggtgggac ttcttatcac tgcacttgcc ttttggctaa tgattaccag
     7681 tataatgctg ctgttgcaga aaaacaaaaa caacattctt aaactcaagc tactgggcta
     7741 ccatcccgaa aagatttcgc gcccctacta ccttttatcg ctggtaatga ttggagctat
     7801 tgccttgtta tcggtaattc cgttatactt catccgcagc ttttataccg gtaaaatgtt
     7861 ggctatgggt tatgaatcag cagcaacata cacacctatt cttgcgctgc tcatgctttc
     7921 gtttgttatt gctctgctac ttatttgtct gataagtatt cgcatcagag tacgacagtt
     7981 tataaaataa tcctacccac atcacattca ttttcaaaca catacagaat cgataagatt
     8041 ttattaatgt acaaaacttg attcttattt agaattattt taaatttgta gaaatttatt
     8101 gagcatgtta ttatcggaag cacatgttgg cgaaagttat gttgttgccc gcctctcagg
     8161 cagatggggt attcgtcgta aactgattgc gcaggggtta gagcatggag taaccattag
     8221 aataaaatca atcgacagcg aaaattttca tgttgcagtt aacggtcata ttggaagacg
     8281 cagattaccc ctcactctgg ctccattcgt tactctggtt tctgccaacg ataaaaacct
     8341 gcatgaaaaa ctcgatgact tatgcaacag catcccggta atcagttttg ggaatcccga
     8401 aatattgaat tcggtttttg gaacagacat cgccgatttc agggagtcga ttgtttttca
     8461 acacgatcat gaaatccaca tcacccacct ccctaacaca gatactatag ctggcaatgg
     8521 tgaaaatgaa acaaaaatac gtgaactcat tacgcaagaa taccccgatc tgattgttgg
     8581 aataatcgat gcctccaggc tgaaagagga cttgtattta gcatcttttt tcatcgacat
     8641 ggatgtgaaa atgatgttga ttattacagg tcttgaacac cttgatgaac aaggtctaaa
     8701 gctcgattac gagcatctgg gaaaaatgct ggggattcca atactcccat ataaaaaacc
     8761 agaagataaa ccgctgatat taagaacatt actaaacatt catcacaaca ccgagccgta
     8821 cgtacggagt attgatataa actacggcga cgaattggag cgatcgatca ggcgaatcga
     8881 gaagcatatc gtaaaagata tggcgctgca tgatgagata gcaccacgct acaccgcatt
     8941 gcgcttactc gaaaacgatg aaaacactca aaagaatgtt tcgtattgta acaggtgcac
     9001 aaggcgttcc tgccttaccg ggtcagaaat aaaacgtctt gaaaaactgt tcgatctgcc
     9061 ggtaatgcaa attgtacatg aagcaaggat gagcttcatc aatggtggtc tgaaagaaaa
     9121 cctgacccat ggcaagtcga aaaaagcagt taaaacagag aaaaggctcg atgccattct
     9181 tacacataaa atatggggta ttccaatatt tttcggcttt attctcctaa cattctttgc
     9241 cacatttcaa ctagggaaat atccttcgga ttggctggaa aaaggcatac tgatgctgat
     9301 ggattacctt ggaaagacaa tgcacgaagg tgcactgaaa agtcttttga tcgatggtgt
     9361 actgggcggt gttgggggta ttttaattta tatacccaac atctttattc tgtttttctt
     9421 catcgggttt ctcgagataa ccggctatct ttcgagggga gcatttcttg tggacaaata
     9481 catgcaccat atcgggcttc atgggaagtc gtttattccg ctaatcatgg gttttggatg
     9541 tactgtgccg gcaataatgg caacacggat gctcgatgac cgaaggaatc gcctgctgac
     9601 aatgctgatt gttcctttta tgtcgtgcag cgccagattg cccgtttatg tgctgatgat
     9661 cgctgcattt ttcccggaac accctacgct catattattt ataatatatt caatcggtat
     9721 tctgtttgca atcatcacgg catttctgtt caataaaaca atattccgga agaaagaagt
     9781 accttatgta atgcagctga ccaactatca taagccgtca ttaaaaatgc ttacgcatta
     9841 cacatggatc aggggacgcg aatatctgaa aaaaatagca ggaatcatac tgcttgcctc
     9901 tgttttgata tgggcgctgg gctattaccc gagagatata aaatattcga aaaactacga
     9961 tactctggta acagaggcta ctgacactaa agaagttaaa aagctgaatc ttgcacgtga
    10021 agccgagcaa caggaaaaca gctatattgg gaaaatcggg aagttcatac aacctgccat
    10081 ggaacccctt ggcttcgact ggaaaatgag tatcagcgtg ctcacaggca ttgcagggaa
    10141 agagattgtt gtgagcacta tgggggtatt atatcaggca tccgacgaaa atggtcatag
    10201 tgccgagagt ctgccggtga aactcagttc cgaaaagcat acatccggca acaatgcagg
    10261 taaacccgtt tttaataaag tgtcggcatt cgcttttatt attttcattt tattatattt
    10321 cccttgcatg ggagttgtgg tagcaatatg gaaagaatca gaaaaaatca aatggtcggt
    10381 atttacggtg atttatacta cggcactcgc gtggatcatg gcttttctag tgaatcaaat
    10441 cgggagcgca ttgtttttgt aattgagccg gtcatcgagc gtagtcgaga tgtcggcgtg
    10501 atgctgccga tatttaaaag attgtctttt caaatagcgg gcacatcctc tacgccattc
    10561 cggtcttcga gccggtcatc gagcacagtc gagatgtcga cgacatatta cctaaatgta
    10621 aagaattatc tttaaataac gggcacctcg actacgctcg gtgtccgcat tatttaatca
    10681 atttattaaa taaacactac agatgtacct gtcactattt ctccaaggtt tatagaatta
    10741 tccgttccat ttcgacaacc cacatttcga ctttcgaggt taacccatat gatttgtttc
    10801 actttactat aatcaaagtt ttttcttgga ttctttatag tttgaggtgt ttttgcattt
    10861 acttaattct ggtaattttt caacagagcc aattatcaaa gcctctttct ttttgtgtga
    10921 ccaattttgg acctgctttt cgcgataaaa tgcttgatcg atatattgaa actcttcaaa
    10981 atacaccaat tctacgggaa gatgcttacg agtgtagttt gaacctgttc cgctcttatg
    11041 ttgattaacc cgttgttcta aatcaatagt acttccggtg tagaattttc cattggcaca
    11101 tttcaatatg tacatgaacc ctttcatgtt tcaaagttaa tgattcaatt tttaataaat
    11161 gtgtgttttt tcatttatta ctcgggcacc tcgactacgc tcggtgtccg tatatccaat
    11221 aatcaagccg gacatcgagc gtagtcgaga tgtcggcgtg atgtcagcat aatatcggcg
    11281 taattttacc gaaatataaa gattgttttc aaataacggg cacctcgact tcgccattcc
    11341 ggtcttcgag ccagtcatcg agcgcagccg agatgtcggc gacatattac ctaaatgtaa
    11401 aagaatatct ttaaataacg ggcacctcga cttcgccatt ccggtcttcg agccggtcat
    11461 cgagcgcagc cgagatgtcg gcgacatatt acctaaatgt aaaagattat ctttaaatag
    11521 cgggcacctc gactacgctc ggtgtccgca tgatgaaatc aatttcttta ataaatacta
    11581 tatgaataac cgtctccgat tactctagcg ttaacggacg tctcgactac gctcgacgtc
    11641 cttaaaaaaa agccgcattg tgcggctttt caaattgttt tacttttttt tatcggtttt
    11701 gggtggttcg gcaggaggta tttcttcggc ttttacattg gagtactggt cggtctgtac
    11761 tttacctttc agatattcgg gcaactccat acctgccatt ttaaacagat cctgcattgg
    11821 tggaattgag cccagcattc cagaaaggaa attagccgta gccggtgact tgccgtcttt
    11881 accactcgac ataccatccc aaacagtgat tttatcaatt ttgatatttt taatggcttc
    11941 aacctgagtt ttcaccatat cgggcaattt atcggcaatc ataagcatta cggctttctg
    12001 aggatcgcta ccggcagctt ttaccagctt ttcgaaacca tcggcctgct tggtaagaat
    12061 ttcgtaaata cctttacctt ctgcttccat tttcatgaat atagcatcgg cctcgccttt
    12121 ggcatggcga cgggtttgtt ctgcgatagc ttcagctgca atttctattt tttccttatc
    12181 aatttttgta ggaacaatta tatcagcctg ccgtgttgct tcctcgcgct gagcacggat
    12241 gatttctgca gatttttcgg cagaatacga ttcttcgaga gctcgggctt tgttcacctt
    12301 ttcggcagct actgcctttc gttctgcatc ggcttctttc tcgcgtctgt tcgacgatga
    12361 gtttgcaatg gcaatagcag ccatattttc accatcaact gcagtagcat ttgcctgaga
    12421 aacctgaata cgctgatctt tcatagcatt agcttcacca atagaaccat cgcggttttt
    12481 ctcggccact gactttttag cttcgttgat agcctgagct gctgcttcct tacccagtgc
    12541 ttcgatatat ccactttcgt ctttaatgtc ggtaacattc acgttgatga gtttcagacc
    12601 gatttttttc aattcctgct ccacattggt cgaaacattg gcaagaaatt tatcacgatt
    12661 agagttaatc tcttcaatat ccatggttgc aaccacaaga cgcaactgac cgaaaataat
    12721 atctttggcc aaattgctga tgtcttctct ggcaagacca agcaaacgtt ctgcagcatt
    12781 cgacataatt ccaacttccg tagaaatacc gacagtaaaa attgagggaa catctacacg
    12841 tatattctgc ttgctcaagg cacttttcag gttaatctca atcgatatgg gtgtaagatc
    12901 cagaaactga taatcctgaa ttacaggcca tataaacgat gctcctccat gaatacatct
    12961 cgatgaacgc gactcatcgg tacctttctt gcccactttt ccgtaaacga ccagaattcg
    13021 gtcggacggg cagcgtttgt acctgcggag gaaagttatt aaaagaataa acaggaacag
    13081 tactcctgca ctgacaatga ctaaaatgta aggttccata attgtgagat tttaatttta
    13141 taagcgggtt acaagaagaa tttcattatt gattatatcg gtaactttaa caagagtgcc
    13201 gttgggtatt gcttcctcat cgtcggtgat ggcatccagc gtctggaaac catgaagggt
    13261 aagctgaact tttccctgcg cggctctttt accaggaata ggaagataga ccgtagcggt
    13321 ttttccaata gcattgctca ttttcattga accgtcttcg gctaacttcg acaggtaata
    13381 aatcagcagg gccatgatca gcatcatcaa taaacctgct gccagagcta taaccagcga
    13441 gagaattacg ctcagaccgc caacgctaca ggcaataccg gtccatccaa atatggtaaa
    13501 aaacccaaca atattcttga tgctaataaa ctgaaagcca ataccggcat cgctatctac
    13561 ggccacatca gcatctccgg tagaagcacc ggcttcaaag tcgccgccaa tcaaagtcat
    13621 gatcaactga atgatgaaaa caacactgaa tggtacagct atgcaccaat aaattttttc
    13681 ggccagctcc attgcctgcc accaattttc gaaacttaac agcatagtaa ttcttgtctt
    13741 taaataatct cacaagataa taaacctttg tatataaacc gcaatttaaa tcaaacatta
    13801 tttgcattat tttgttaaat aatgttaaca ataaggttta cttttgcaaa aaatttttgc
    13861 gttatgaaag ctttagtcat tggcgctacg ggacttacag gttcaagcct tcttgaacaa
    13921 ctgttgtcgg acactcattt tactgagata acagttcttg cacgccgctc aactcaggtt
    13981 gtttcggaga aagttacgga gcataccgtc gattttgata agcctgaatc atggaaacac
    14041 ctgataaccg gcgatgtgct gttttcggca cttggcacta cgcttaaact ggccggaagc
    14101 aaacaggcac aatataccgt ggattatcac taccagcttg ctgtggcaaa agctgcagcc
    14161 gaaaacggag taggacgcat cgtactcatc agttcgtatg gtgccgatgc caaatcgggt
    14221 gttttctacc tgaaaatgaa aggagaactc gaagaaagca ttatgcaatt gggattcaaa
    14281 aactgtgtca tgttacgtcc cgggcaaatt gatggaaaac attcgccgca acgtgccgga
    14341 gaaaagtttg ccctgaaagc cgtgaaagcc ctgaacagat gcagcatttt gaaacgatat
    14401 acgcctatta caccgcaaaa acttgccacg gcaatgatca atgctgcgct gctgaacgaa
    14461 gagaaaaaca tcactattgt tgaactggag caggtacata agcttgccga aaaacagatt
    14521 gtatgagggg aaacaacgga atactcgtac ccatttttat catcatcctg agcttgctta
    14581 ccgactggta tgtactgcag ggattaaaaa ccatcatact gcccatacga cagcccggaa
    14641 taaggcaggc tatcatcatc ctgttccgga tattttcgat cggggtgatt gtgggtacca
    14701 ttatcggtgg atattttgta atggtgaagg gagaatttac cctgatgttc aggattttta
    14761 tgcatatttt cctgatctat ttcaccgcca agattgcttt tattctgttt ttatcgttcg
    14821 aagatattgt gcggcttttc cgcggcacca tcagcctgat atcttcaggt acgttgggtt
    14881 cgtttctgcc accccgtagc ttcggggtga gtgtggcggg tttgtcg
//
LOCUS       DAOOQP010000074        14914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4907_length_14914_cov_5.622788, whole genome shotgun sequence.
ACCESSION   DAOOQP010000074 DAOOQP010000000
VERSION     DAOOQP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14914
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4907_length_14914_cov_5.622788"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLK75_08450"
     CDS             <1..285
                     /locus_tag="PLK75_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39316.1"
                     /translation="LIVFIQLNQWSNFRGVLYTTLNLNPERIISAFNTYMKLSQLSVR
                     RKDFLVNLEEKINDSDFRKDIFGLIHPSITCNIDEAYTIVRDSIVMLLME"
     gene            complement(328..2226)
                     /locus_tag="PLK75_08455"
     CDS             complement(328..2226)
                     /locus_tag="PLK75_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39317.1"
                     /translation="MKLISIAVFLLLCMGTYAQERFNKIIYDNDPHIGNTVIVCDSTY
                     VMLTGTLVNGERGIALTKVDKQGNVVLQKKYRKDNYLCYEGRYNCMKYDSITNLFYLC
                     GLAMTYDYQNKYPFFVELNENLDTIIFKIIEVDSVYTTVDVIKLDNTHYAITGQIITP
                     DDTEMGLMIYGYDETVLKIGYGVPDYTSNEASRKVIKTTNNEILLGGFSFGFNSGNFA
                     QDWYLVKTDSIGNMIWQQHYGNPVINDGYISGMLQVSDTSYMLCGGQGICNDNGDAIL
                     EGALRMVDTSGNLVWEKFYRHYLYSQMQDEIKYSMTYFTDLVELEDSGYAGLLYYADY
                     YTNGRFLVMKLTSDGKIIWTRRLQAYEELGLSEPSSIKQTHDSGFIIQGYANYPSGQQ
                     FFLIKTDSLGVEGSVYPQPPPELINCTGLPDTLYCEQTYSCKLQVQGKSAPYTLNFST
                     GEQIDSLYYPPVFLSATIETGTDFDVLVGLTNYTHSYDSATIFNETQNEESIPDYIEI
                     PFSITVPENYTDTTISVGLTNGIGESYEITLPVYVDCNVSSEPLNETAAIQVYPNPAT
                     DYLQIQLPDENETSFVKILNLQGQTVLVQPLQSQTTRLDISGLAPGTYMVRLYYGHRI
                     ENVRMEKQ"
     gene            complement(2369..3313)
                     /locus_tag="PLK75_08460"
     CDS             complement(2369..3313)
                     /locus_tag="PLK75_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOY39318.1"
                     /translation="MANKCLYCYEPVEDGANFHKTCSMEFFGTAAAPNIEYSLNQMDE
                     LAKNVVERSVSVPGVQAKLSLSLVRKTKETADNRLTIVGALGGQYIFKPPSEHYREMP
                     QNEHLSMRIARSFGIRVVPSSLIRLASGELSYITKRIDRSDSGEKIHMIDMFQITEAN
                     DKYKSSMEKIGKAIGEYSVNTLLDKVFYFELVLFSFLTGNNDMHLKNFSLIKSSSGWM
                     LSPAYDLLNVAIVLPEDKEELALPMRGRKKRFTRNDFEQFGTDLGLTDKQIQSIFRRM
                     IKNKAHAFRLIDISFLSDEMKMAYKSVMEAKYHQLEMM"
     gene            complement(3303..3644)
                     /locus_tag="PLK75_08465"
     CDS             complement(3303..3644)
                     /locus_tag="PLK75_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOY39319.1"
                     /translation="MRQGQVFYKDIPAGILTETDDGEYVFRYDAEYINTHPDDFITFT
                     MPVSQWAYTSPRLFPFFEGLIPEGWLLDIAASNWKINKSDRMGLLLACCQNCIGAVSV
                     KPLTAGGSDGE"
     gene            complement(3641..3850)
                     /locus_tag="PLK75_08470"
     CDS             complement(3641..3850)
                     /locus_tag="PLK75_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY39320.1"
                     /translation="MKTLAEFVKERRKEVNLTQSEFAERAGVALTLVRKIEQGKTNMN
                     LDKVNLVLQMFGHELAPVSSKELKK"
     gene            complement(3938..4120)
                     /locus_tag="PLK75_08475"
     CDS             complement(3938..4120)
                     /locus_tag="PLK75_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39321.1"
                     /translation="MGKFKMQTSKQINIARNTAGRKNWQTDYDDRIIRNDAEYQRIVE
                     YIRNNPGEWFGRSLYA"
     gene            4307..5389
                     /locus_tag="PLK75_08480"
     CDS             4307..5389
                     /locus_tag="PLK75_08480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOY39322.1"
                     /translation="MQKINFDCRYFRGGIPCKPNKQHRVECGNCSYYQPISKRILIIK
                     LGAMGDVIRTTPLIAAYRKKFPDCHISWLTLTPEILPSSEIDSIYKWDVGSVFILSNR
                     KFDIAINLDKEDEACMLLSKLEATEKYGFIWNDNHIEAATPAAMHKLVTGLFDHISQD
                     NTQHYMQEIFDISHLPFNDEEYLINLNEKLAQQWAMHFETISGGKKIIGLNTGCGNRW
                     LTRLWPDEYWITLINQLLRQGFFPVVLGGPQENDKNTMLSEKTGCYYPGTFSLEEFIA
                     LSKSLDLVVTQVSMMMHIAMALKRKIVLMNNIFNKHEFYMYNRGIIVEPESGCDCYYG
                     NSCSRERSCMYDLKPEIILKKIKELL"
     gene            5389..6510
                     /locus_tag="PLK75_08485"
     CDS             5389..6510
                     /locus_tag="PLK75_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39323.1"
                     /translation="MQIAYLSTFYPFRGGIAQFNASLLQTFVDMGHRAQAYTFTCQYP
                     GFLFPGKTQMVTTGDNAMVIDSTRVLNTINPFSYCSTAKRINREAPDLLLMKYWMPFF
                     GPSLGTVAGRLKNQTKSIAILDNFIPHEKKFFDTAFNSYFVKRVNGFVAMSDKVKTDL
                     LNHKPDAHVMLMPHPVYNHFGQRTDKESAQQKLGLDPKKKSLLFFGFIRTYKGLDNLI
                     RAFNLLDESYQLIIAGETYGSFEKYANLIEENKFKSNIKVYNDYIPDNAVANFFSASD
                     VCILPYRSATQSGITSISYNFDVPVIATNTGGLAEFIENGSTGLIIADSNPETIADGI
                     RRFYADCNISAMQQRISEIKQNLSWEHFAGEIIKFSKTL"
     gene            complement(6526..7917)
                     /locus_tag="PLK75_08490"
     CDS             complement(6526..7917)
                     /locus_tag="PLK75_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOY39324.1"
                     /translation="MNQRFAIIDVETTGGSAGQDKITEIAIVVTDGEQIVEQFSSLVN
                     PECMIPAYIVGLTGITNEMVENAPCFYQLARKIVELTENTIFVAHNAAFDYNFIRCEF
                     LRLGYKFRREQLCTVKMSRKLIPGLPSYSLGNLCEKLGIVINNRHRAEGDAVATYHLF
                     KLLYKIDREQYSGKNMQGFSTKGLNRNLDIATLKALPEAAGVYYFYNENTDLIYIGKS
                     KNIYRRVITHLNNETTSKGVQLKSEIAGVSFELTGSELIALLKESCEIKRNKPRYNKA
                     QRRTKHQWGLYYDVNENGYLQFSIRKNDDGYDVPLDSFVSQGAAKDNLFSLCNEYSLC
                     QKHCGLYQSAGACFYHALGDCRGACIGEEPAESYNDRATQVLKRYGLENENIFIVTSG
                     RNEEEIGVVSVKNGKYMGYGFCDIQHQNNPELLSDCIASYPDNRDARQIIRTYLKTNR
                     DYRLISMQADVCS"
     gene            complement(7919..8242)
                     /locus_tag="PLK75_08495"
     CDS             complement(7919..8242)
                     /locus_tag="PLK75_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39325.1"
                     /translation="MECPNCHSKGSVFKRIFSRKKGSKNRFCVFCGAEVKITYNWKRI
                     FILCIIVMLCLFVVNIVIQLLGAYGLTSGFSGGLAAAVIAIFMRKPPYTVIELVNKPG
                     KKHRH"
     gene            8584..9426
                     /locus_tag="PLK75_08500"
     CDS             8584..9426
                     /locus_tag="PLK75_08500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOY39326.1"
                     /translation="MKFLTNKGFLGIVLLIIIGAVCSWLLDGTLLSLSGVWRLLFAIG
                     VTLLPGLLWLWFFYMEDKYEKEPKQMLLGVFALGGLTAYALSKPITALFVPEVSVFDQ
                     NLLSQFLVAFLLVGMIQQLVIFLTVRYSIYFSNEFNEPADGVIYSIAAGLGFAAVYNF
                     EYLVQMDAMNLGIVITRIINFYLVMAIFSGIMGYFIGFAKFSKAKNCGREMFMAIGLI
                     LAAFLNGLYWTVLTNVQGMNFSILKELAISAGSVIVIYLIMYFLLNRSVEASPFKNPE
                     NKPQ"
     gene            9466..10032
                     /locus_tag="PLK75_08505"
     CDS             9466..10032
                     /locus_tag="PLK75_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39327.1"
                     /translation="MENQKLSYISWAVLIGAVLILAGWFLTRSSNANTKPFSFAINDS
                     VSKKAVTVGFEYPKDWNEMATDKAESKLGSLKTPKTYVLIDKQTLTMMGLKGAQAVVN
                     QRNFEEYTEISSEDLKINDLNAVKIGFSYTDNGINMQACQVLVDLKTNAKDMIVITYF
                     APEKDYAKSIGDFGKLIKSISITSSNLK"
     gene            10043..11302
                     /locus_tag="PLK75_08510"
     CDS             10043..11302
                     /locus_tag="PLK75_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOY39328.1"
                     /translation="MKKHIIISLFAALLLLMQSQTFAQINPVAISDNYYKGIVKILLF
                     DPVLAQVDEETLGYLGRGSGFFVTTDGVIFTNRHVIEQCVYGYILYQDKKGEYDIVTY
                     SKGIEKDPSVKCVAYSGYTVPIVQVYHGRGPNDYKLYLAEVLTLSDAYDGAVIRIVSD
                     MDGNKPTAPFTALPIGNSDNCKQGEDLVVMGYPAQYDENYDLALKDMITFTKGYNSGL
                     DYVFNEDYGFIKTDAAINGGNSGGPVFGNDNKVIGIASAKGIKTNIGLVGGINGMYYV
                     VAPNATIFKTLISKGLTPPDKAENITVTKGDAKPMPEFVSCDQQTSKAKQTTVTGIVK
                     SADTGKPIKDAVVGLLIYDAAKDDFIVVSGGYTGSDGKFTMEPDVTLDTDYGIAVMAD
                     GYQDLVDVITITSSSNYLTITLAKQRK"
     gene            11401..13140
                     /locus_tag="PLK75_08515"
     CDS             11401..13140
                     /locus_tag="PLK75_08515"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOY39329.1"
                     /translation="MAEADLIAKVRNKATAWLNDNIDAESACQIEDMLHKNDDTLIES
                     FYKDLEFGTGGLRGIMGMGSNRMNRFTVGMATQGLANYLIKTFPKEKIAVAIAHDSRN
                     NSRSFAQNAADVLSANGITVYLFDELRPTPELSFAVRTLECKSGIVITASHNPKEYNG
                     YKVYWADGGQIISPHDKNIIAEVRNISRISEVKFTGNSALIHPIGSDIDTLYIEKVKS
                     LSLNPEVIQRQQKLSIVYTPIHGSGVNIVPMALQSFGFTNIIRVPEQDIPDGNFPTVH
                     SPNPEEAAALSMALEYAKKAGSEIVMGTDPDADRVGIIVRDSNGEYVLLNGNQTASVL
                     LYYLISEWHRKGKIDGRQFIVKTIVTTDLLAEIADSFNVKNYDVLTGFKYIAAIIREY
                     EGKMEFIGGGEESYGYLAGDFVRDKDAVISCALIAEVAAWAKDNGKTIIDILKEIYVR
                     FGFYKEKMISVTRTGKSGAEEIAAMMDSFRNQPPESICGTDVTIIHDYEKQASIDMLS
                     QLRYTIPLPKSDVLQFELKDGSKITVRPSGTEPKIKFYFSLKASLQRVEDYDTVNVEL
                     DQLVEKMAKEIIR"
     gene            13141..13557
                     /locus_tag="PLK75_08520"
     CDS             13141..13557
                     /locus_tag="PLK75_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOY39330.1"
                     /translation="MKSYRKELWFRTETRRKLINITPKIIQCLTESGIREGFCLVNAM
                     HITASVFINDDEAGLHHDFEVWLEKLAPEKPHSQYRHNGYEDNADAHLKRSVMGREVI
                     IGVTEGELDFGPWEQIFYGEFDGMRDKRVLVKIIGE"
     gene            complement(13564..>14914)
                     /locus_tag="PLK75_08525"
     CDS             complement(13564..>14914)
                     /locus_tag="PLK75_08525"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOY39331.1"
                     /translation="AFRIEVTVTGHENGLLYFGSHYGPEYQVIDTIQTDSKGHAVFFG
                     DFVLPQGVYFIVIPPKTRFDFLVLDDQDFSITTGLDNVLGKLIMTGTEKPDLFFGLQR
                     DIAAINLASSELDIKKQYYANSGQANADEIKRTEDSLKNRRMVVYKYYKTQDNNGFLS
                     KMMAAMLPPEFPPYVLQWQTEDPARYYYYYKDHYFDAVDFSDERLLRTPEFVFHRMLD
                     EYSKFFLSTRVDSIENVYKDVDALIAKASVNIDFEKYIVSYLVDHFENPSVIGMDAVF
                     VYLANNYFLNGKIKWADEKAIDMIRSRVDEMKYNMLGAKSHNLHLKRIDGALFETDSI
                     QARYIILWFWERGCSLCDEQTPVLHSSYEEIKKMGAELIAINIQSDKNAWEDYVTNMK
                     LSWVNACSAGSTDDFFIYYGTTRTPRLFILDSNKIIVAKDVKPKEIVRYLKYLDTKK"
BASE COUNT         4349 a         3135 c         2993 g         4437 t
ORIGIN      
        1 ttaattgttt ttattcaact taaccagtgg tccaattttc ggggagtatt atatacaaca
       61 ttgaacttaa atcctgaaag gattatttct gcctttaata catacatgaa actatcgcaa
      121 ctttcggtaa gaagaaagga ttttttggtt aatcttgaag aaaaaatcaa tgattctgat
      181 ttcagaaagg acatttttgg tttgatacac ccatccataa catgtaatat tgacgaagcc
      241 tatactattg tgagggattc aatagtaatg ttattgatgg aataagaatt aaatttgtga
      301 ggcgttggct atttgttcgt tgtcttttca ttgtttctcc atccttacat tctctatccg
      361 gtggccatag tataacctca ccatgtaggt tccaggggcc aggccgctga tgtctaaccg
      421 ggttgtttgt gattgcaatg gctgcaccaa cacggtttgc ccctgcaggt tgagtatttt
      481 cacaaacgag gtctcgtttt catcaggcaa ttgtatttgt aggtaatccg ttgcagggtt
      541 cgggtaaacc tgaatggcgg ctgtttcgtt tagtggttct gaacttacat tgcaatcgac
      601 ataaacagga agtgtaattt cgtagctttc gccgatgcca ttggttaagc ctacgctaat
      661 ggtggtatca gtatagttct cagggacagt aattgaaaac gggatttcaa tataatcagg
      721 aatcgattct tcgttttgcg tttcgttaaa tatggtcgct gaatcgtaag agtgagtgta
      781 attagttagt ccaactaaca catcgaaatc tgttccggtt tcaatcgttg ccgataaaaa
      841 aacaggagga tagtatagcg aatctatttg ttctcctgta ctaaaattaa gtgtgtaagg
      901 cgccgacttt ccctgtacct gcaatttgca gctataagtt tgttcacaat acaaggtgtc
      961 tggcaaacct gtgcagttta ttagttcagg gggaggctgg gggtaaacag agccttcaac
     1021 tcccagactg tcagttttta ttagaaagaa ttgctgtcct gatggataat ttgcataccc
     1081 ttgaattata aatccgctat catgtgtctg tttaattgac gatggctcgc taagacccag
     1141 ctcctcatag gcttgcaaac gtcttgtcca gattatttta ccgtctgatg tcaacttcat
     1201 taccaaaaat cttccattgg tgtaataatc tgcataatac aataagccgg cataaccgct
     1261 atcttccaat tcaacgagat ctgtaaaata ggtcatcgaa tattttatct catcttgcat
     1321 ttgactatat aaataatgcc tgtaaaactt ttcccatacc aggtttcccg acgtatctac
     1381 catacgcaaa gctccttcaa gaatggcatc tccattgtcg ttgcagattc cctgcccacc
     1441 acacaacata tagctggtat cactgacttg aagcattccc gatatatatc cgtcgtttat
     1501 cacagggttc ccataatgtt gctgccatat catattccca atactgtcgg tctttactaa
     1561 ataccagtcc tgagcaaagt tcccgctgtt aaagccaaag ctaaaacctc ccaataatat
     1621 ttcattattt gttgttttaa ttacttttct cgaagcttca ttagaagtat aatccggaac
     1681 accatagccg attttcagta cagtctcatc ataaccataa atcattagcc ccatctctgt
     1741 gtcatcaggt gtaataattt gaccagtgat tgcataatga gtattatcta gttttataac
     1801 atctacggta gtgtaaacag aatcaacttc aattatttta aaaataattg tgtctaaatt
     1861 ttcatttaat tcaacaaaaa atggatactt attttgataa tcataagtca tcgctaatcc
     1921 acataaatag aataaattag taatgctgtc atatttcata cagttatatc ttccttcata
     1981 acagaggtag ttatctttcc gatatttctt ttgcagaact acattgccct gtttatcaac
     2041 tttggttaaa gctatccccc gttctccgtt taccaaagta ccggttaaca taacataggt
     2101 gctgtcgcaa acaatcactg tattcccaat atgcgggtca ttgtcgtaaa ttatcttatt
     2161 aaacctctcc tgcgcataag ttcccatgca caggagaagg aataccgcta tactgatcag
     2221 tttcatcggc ctacattttt gtgaattttt ttaccgcatc acccattata gatgaaaaac
     2281 aaattcacca tgctaagata caaatatttg ggttgttggc gttcgataat ggttttttcg
     2341 caaggttttt tatgcaggct gccttgcatt acatcatctc caattgatgg tactttgctt
     2401 ccatcaccga tttgtaagcc attttcattt catcggagag gaatgagata tcgattaaac
     2461 gaaatgcatg ggctttgttt tttatcatcc tgcgaaagat gctttgaatt tgtttatcag
     2521 taagccccaa atcggttccg aattgctcaa aatcgtttcg tgtaaatcgt ttttttcgcc
     2581 ctctcatcgg aagtgcaagc tcttctttat cttcgggcaa tactatggcc acatttaata
     2641 aatcgtaagc cggtgatagc atccagcccg aagaactttt gattaacgaa aagtttttga
     2701 ggtgcatgtc gttgttccct gtcagaaacg agaaaagaac caattcgaag taaaacactt
     2761 tatcgagcaa ggtattgact gaatattctc caatggcctt cccgattttc tccatcgagc
     2821 ttttatattt atcattggct tcggttatct gaaacatatc tatcatgtgt atcttttcgc
     2881 cggaatcgct gcggtcgatt cgtttcgtta tatacgacaa ttcgcccgat gccaaacgga
     2941 ttaacgacga aggaactacc ctgataccaa aacttcgtgc aattcgcatg ctgaggtgtt
     3001 cgttttgagg catttcgcga tagtgttccg aaggtggttt aaagatatac tgtccgccca
     3061 atgcacctac aatcgtcaga cgattatctg ctgtttcttt cgtttttctc actaacgaca
     3121 aggatagttt tgcctgaact ccgggcaccg aaacactgcg ttccacaaca ttctttgcca
     3181 actcatccat ctgattcaac gaatactcga tgttgggggc tgcagctgtc ccaaaaaact
     3241 ccatagagca agtcttatga aagttggctc catcctcaac tggctcgtag caatacaaac
     3301 atttattcgc catcgctacc tcctgcagtt aaaggtttaa cactaacagc cccgatgcaa
     3361 ttctggcaac aggccagcaa caaacccatg cggtcgcttt tattaatttt ccagttcgag
     3421 gctgcaatat cgagcaacca tccttcggga ataagtcctt cgaaaaatgg aaacaatcgc
     3481 ggcgatgtat aagcccattg tgatacgggc atggtgaatg tgatgaaatc atccggatgc
     3541 gtatttatgt attcggcatc gtagcgaaat acatattcgc cgtcgtcggt ttcggttagg
     3601 atacccgccg ggatgtcttt ataaaaaact tgtccctgtc tcatttctta agctctttac
     3661 tgctaaccgg tgccagttcg tgtccgaaca tttgaagtac cagattcacc ttgtcgagat
     3721 tcatattggt tttcccttgt tctattttcc gaactagggt cagggcaaca cctgcacgtt
     3781 cagcaaactc gctttgcgtg agattaacct ccttacggcg ctcttttaca aattctgcca
     3841 gtgttttcat tatatgctta tgaatataat tatttgcaaa tatacacaat tatatgcaat
     3901 tagatatagt tggataaaaa atatttaaat tatatgttta ggcatataac gacctgccga
     3961 accattcccc gggattattc cttatgtatt ccacaatacg ttggtattcc gcatcattgc
     4021 ggataatacg gtcgtcgtaa tcggtttgcc aatttttccg tcccgccgta tttcgggcaa
     4081 tattgatttg tttcgatgtt tgcattttga atttccccat gattttcggt attaacattt
     4141 caacaggttc aatgcggcgc atgttgcggc gcgataaacg gtatttttcc tgggttgaag
     4201 tccgtttaaa acataaaaat gaccgtttct gtctacataa tttcttggac ttgaatctgg
     4261 cagatttgaa tgttttttct acttttgtag taagtatagt tttacaatgc aaaaaattaa
     4321 tttcgactgc aggtatttca gaggcggtat tccatgcaaa cccaacaagc aacacagggt
     4381 tgaatgtggc aattgcagct attatcaacc cataagcaaa cgtattctta ttataaaact
     4441 gggtgcgatg ggcgatgtta tccgcaccac accccttatt gctgcgtatc gcaaaaaatt
     4501 tccggactgc cacatcagct ggcttaccct taccccggaa attcttccat cctctgaaat
     4561 tgacagtatt tacaaatggg atgtaggctc cgtttttatt ctttcgaacc gtaaattcga
     4621 tattgctata aacctcgaca aagaagacga agcctgcatg ttgctttcga aactggaagc
     4681 aaccgaaaaa tatgggttca tctggaacga taatcatata gaagctgcaa cgcctgctgc
     4741 catgcataaa ctggtaactg gtttattcga ccatatttcg caggataaca cgcagcacta
     4801 catgcaggag attttcgaca tttcccacct gccattcaac gacgaagaat atcttatcaa
     4861 ccttaatgaa aagctggctc aacaatgggc aatgcatttt gaaacaatat cgggaggaaa
     4921 aaaaatcatc ggcttgaata cgggttgcgg gaatcgctgg ctaacacgcc tctggcccga
     4981 tgaatactgg attactttaa ttaaccaatt gttgcgtcag ggtttcttcc ctgttgtttt
     5041 gggtggtccg caggaaaacg ataaaaacac gatgctgtcc gaaaaaacag gctgttacta
     5101 tccgggaacc ttttcgctgg aagaattcat tgcgctttca aagtcgctcg accttgtggt
     5161 aacacaggtg tcgatgatga tgcacattgc catggcactg aagcggaaaa ttgttctgat
     5221 gaacaatatt tttaacaagc acgaatttta tatgtacaat cgcggaataa ttgttgagcc
     5281 ggaaagcggc tgcgattgtt actatgggaa tagctgctcc cgcgaacgct cgtgtatgta
     5341 cgacctaaaa ccggaaatta tactgaaaaa aataaaagag cttctgtaat gcaaattgcc
     5401 tacctctcca cattttatcc gttcaggggt ggaattgccc agttcaacgc ttcactgctg
     5461 caaaccttcg tcgatatggg gcatcgtgct caagcctata cattcacgtg ccagtatccc
     5521 ggttttttat ttccggggaa aacgcaaatg gtaacaaccg gcgacaatgc catggtaatt
     5581 gatagcacca gagtgctgaa cactattaat ccattttcgt attgcagcac agcaaaaaga
     5641 attaatcgcg aagcacccga cctgctgctt atgaaatatt ggatgccttt tttcggacca
     5701 tcgctgggaa ccgttgcagg gcgtttgaaa aatcaaacca aatcgatagc aattctcgac
     5761 aattttatac ctcacgaaaa aaagttcttc gatactgcat tcaattcata tttcgttaag
     5821 cgtgtcaacg gatttgtagc catgtcggac aaagtaaaaa ccgatttact caatcataaa
     5881 ccggatgcac atgtaatgct tatgccccac cctgtatata atcacttcgg gcaacgcacc
     5941 gataaagaat cggcgcaaca aaaactcggg ctggatccta aaaaaaaatc actgttgttt
     6001 tttggtttca tccggacata taaaggactc gacaacctaa tcagggcgtt taacctgctc
     6061 gatgagtcgt atcaacttat tattgcaggt gaaacatacg gaagtttcga aaaatatgca
     6121 aacctaatag aagagaataa gtttaaaagc aatattaaag tttacaacga ctatattccc
     6181 gataatgctg ttgccaactt tttctcggct tccgatgtgt gcatactgcc ctatcggtcg
     6241 gcaacacaaa gcggtattac cagcatttcg tacaattttg atgtgccggt tattgctaca
     6301 aacacagggg gtctggctga atttatcgaa aacggcagca caggtctgat cattgctgat
     6361 tccaaccctg aaacaattgc tgacggaatc agaagattct acgccgattg caatatttca
     6421 gccatgcaac agcgtatttc cgaaataaaa caaaacctaa gttgggagca ctttgccggc
     6481 gaaattatta agttcagtaa aaccctctaa aaactccctc gaaaatcagg aacatacatc
     6541 agcctgcatg gaaatcagcc tgtaatcgcg attggttttt aggtaagtcc gtattatctg
     6601 cctcgcatcg cggttgtcgg ggtacgatgc aatacaatcg gaaagcaatt ccggattgtt
     6661 ttgatgctgg atatcgcaaa agccgtagcc catatatttg ccgtttttaa ctgataccac
     6721 tcctatttct tcctcgtttc gtccactggt aacaatgaaa atattttcgt tttcgagtcc
     6781 gtaccgtttc agcacctgcg ttgccctgtc attatagctt tcagcaggct cttccccgat
     6841 acatgcaccc ctgcagtctc ccagcgcatg gtagaaacag gcaccggcac tttgatacaa
     6901 cccacagtgc ttctgacaca acgaatattc gttacacaag ctgaacagat tgtctttagc
     6961 tgctccctgc gacacaaacg aatcaagcgg aacatcgtat ccatcatcat tcttacggat
     7021 tgaaaactgc aggtatccgt tttcattgac atcatagtat aagccccact ggtgctttgt
     7081 tcttcgctgt gctttgttgt aacgtggttt gtttctcttg atttcgcacg attcctttaa
     7141 taatgctatc agctcgcttc cggtaagttc aaaactcacc cctgctattt cggacttcag
     7201 ctgaactcct ttcgaagtgg tttcgttatt taaatgggta attactctcc ggtagatatt
     7261 tttacttttc cctatataaa tcaggtcggt attttcgtta tagaaataat acactccggc
     7321 agcttccggg agcgctttca gggtggcaat atcaagattt ctgttgagtc ccttagttga
     7381 aaatccctgc atattttttc cggaatactg ctcacggtcg attttgtaaa gcaatttaaa
     7441 cagatgatag gtagccacag catctccttc agccctatgg cggttgttta taacaatacc
     7501 cagtttttcg cataagtttc cgaggctgta cgatggcaat cccggaataa gcttgcggct
     7561 catttttaca gtacataatt gctcacggcg gaatttatac cccaatcgca gaaactcgca
     7621 tctgataaag ttgtagtcga aggcggcatt atgcgcaaca aaaatggtat tttcggtaag
     7681 ttccactatt ttccgagcca gttgataaaa acagggtgca ttttcaacca tttcgttggt
     7741 aataccagta aggcctacaa tgtaagccgg tatcatacat tccgggttca cgaggctcga
     7801 aaactgctcg acgatttgct cgccgtcggt aacaacaatc gcaatttcgg taattttatc
     7861 ctgcccggca ctacccccgg tagtttctac gtcgattatt gcaaaccgct gattcatttc
     7921 aatgccggtg ttttttaccg ggtttattaa ccagttctat tactgtatat ggaggttttc
     7981 gcatgaaaat agcaattacg gcagccgcaa gaccccccga aaagccactt gtaagtccgt
     8041 atgctccgag caactgaata acaatattta ccacaaacaa acagagcata acaataatgc
     8101 ataggataaa tattcttttc cagttatagg ttatttttac ttcagcaccg caaaaaacac
     8161 aaaatctgtt ttttgaacct ttctttctgg aaaatattct tttaaatacg cttcctttgc
     8221 tatggcaatt cggacattcc ataatgtcat cctaagatta ttaattaggt atgcaatatt
     8281 acaaaaaaat tggaatgggc atacgacaaa ctttcagggg agttattatg caatttcgaa
     8341 atatgataaa atttgttatc aatttttaaa caatattgcc tcgaataatt gaaattataa
     8401 attcaactgg gttaattttc aattgctaat acattactat tctgataatt ataataaata
     8461 tcgttttcta tttatgaaaa ttaaaaaaaa tggtatataa attgtaaaat gttaacggta
     8521 tttgtttttt taaatagatt atttatattt gctttaatta gtaaacataa aaaccaaaaa
     8581 actatgaagt ttctaacaaa caaaggattc ctcggaatcg tattattaat tatcatcgga
     8641 gctgtatgtt catggcttct tgatggtacg ttactcagtc tcagcggagt ctggagatta
     8701 ctctttgcaa taggtgtaac cctgcttccc ggtctgttgt ggttatggtt cttctacatg
     8761 gaggataaat atgaaaagga acccaaacaa atgcttcttg gtgtttttgc tcttggcgga
     8821 cttacagcct atgctctcag caaacccatt accgctttgt ttgtaccgga agtttcggtt
     8881 ttcgaccaaa acctgctcag ccagttttta gtagcatttc tgttggtagg gatgatacaa
     8941 cagctggtaa ttttccttac agtgcgttac agcatttact tcagcaatga gttcaacgaa
     9001 cctgccgacg gtgtgattta ttccattgct gctggtttgg ggtttgctgc agtttacaat
     9061 tttgagtatc tcgttcagat ggatgccatg aatctgggaa ttgtgattac ccgtattatt
     9121 aatttctatc tggttatggc aattttttca ggcattatgg gatactttat cggtttcgcc
     9181 aagttttcga aagcaaaaaa ttgcggacgc gaaatgttca tggccattgg actgattctg
     9241 gctgctttcc tcaatggatt gtattggaca gttctcacca atgttcaggg tatgaacttc
     9301 agcattctga aagaactggc aatatctgcc ggaagcgtta ttgtcattta cctgataatg
     9361 tacttccttt tgaatcgttc tgttgaagct tcacccttca agaatccgga aaacaaacct
     9421 cagtaatcag taacaagaaa tataaactaa aaaaaaatca gaaaaatgga aaatcaaaaa
     9481 ttatcatata tttcatgggc agtgctgatt ggtgcagtgc tgattcttgc tggctggttc
     9541 ctgacccgca gctcaaatgc aaacaccaag cctttttctt ttgctatcaa tgattctgtt
     9601 agtaaaaaag ccgttactgt tggatttgag tatccaaaag actggaacga aatggccacc
     9661 gataaagcag aaagcaaact gggctcgctg aaaactccaa aaacttatgt tctcatcgat
     9721 aaacaaacgc ttacgatgat gggattaaag ggtgctcaag ctgttgttaa ccagcgaaac
     9781 ttcgaagaat ataccgaaat atcttcggaa gacctgaaaa taaacgacct gaatgcagtg
     9841 aaaatcggtt tctcatacac cgacaatggc attaacatgc aagcatgtca ggttctcgtt
     9901 gaccttaaga caaatgccaa ggacatgatt gtaatcacat attttgctcc tgagaaagat
     9961 tatgcgaaga gtattggcga tttcggcaaa ctcattaagt caataagcat tacatcaagc
    10021 aatttaaaat aaaccataaa ctatgaaaaa gcacatcatt atcagtctgt ttgcagccct
    10081 gctactctta atgcagtcac agacctttgc gcaaataaat ccggttgcaa tttccgacaa
    10141 ctactacaag ggtattgtga aaatactttt gtttgaccct gtgctcgctc aggttgatga
    10201 agaaacactg ggatacctcg gcagaggctc cgggttcttt gtaacaaccg atggcgttat
    10261 tttcacaaac cgccatgtga ttgaacaatg tgtttacgga tacattctat atcaggataa
    10321 aaagggcgaa tacgacattg tcacctacag taaaggtatt gaaaaagacc cctcggtgaa
    10381 atgtgtagcc tacagtggtt acactgttcc cattgtgcag gtttaccatg ggcgtggtcc
    10441 gaacgactat aagctctacc tggccgaagt acttaccctc agcgatgctt acgacggagc
    10501 agtaatccgc attgtttccg atatggatgg caacaaaccc accgcaccat ttacagcatt
    10561 acctattggc aattccgaca attgcaagca gggtgaagac cttgttgtga tgggatatcc
    10621 tgctcagtat gacgagaatt acgatttggc actgaaggac atgatcactt ttaccaaagg
    10681 atataattcc ggtcttgatt atgtttttaa cgaagattac ggcttcatta aaaccgatgc
    10741 ggctatcaac ggaggaaaca gcggaggacc cgtttttgga aacgacaata aagtaatcgg
    10801 tattgccagc gctaaaggaa tcaaaaccaa tatcggtcta gttggtggta tcaacgggat
    10861 gtattacgtt gttgctccca atgccactat ttttaaaacc cttatttcaa aaggactcac
    10921 cccgcccgat aaagcagaaa atataaccgt taccaaaggc gatgccaaac caatgcccga
    10981 atttgtatcc tgcgatcagc aaacctcaaa ggctaaacaa accactgtaa caggtatcgt
    11041 aaaaagtgca gatactggga aacccataaa ggatgcagtt gtcggcctgc ttatttatga
    11101 cgcagcaaaa gatgacttca ttgtggtaag cggaggttat acgggtagtg atggcaaatt
    11161 caccatggaa cccgacgtta cactcgacac cgattacggt attgctgtta tggccgatgg
    11221 gtatcaggat cttgttgatg taatcaccat tacttctagc tcaaattatc tcacaatcac
    11281 attggctaaa caacgaaaat aattaccagt tatttatact taaaagactt gcccgaaact
    11341 ggcaagtctt ttttttagta atttagacag atttactaca tttacataaa aatttcggat
    11401 atggcagaag cagatttaat tgcaaaagtg cgcaacaaag caacggcatg gctcaacgac
    11461 aatattgatg ccgaaagtgc ctgtcagatt gaagacatgc tccataaaaa cgatgacacc
    11521 ctgatagaat cgttttacaa agaccttgag ttcggtaccg gcgggcttcg tggaatcatg
    11581 ggcatgggtt caaaccggat gaaccggttc actgtgggca tggctacgca ggggctggca
    11641 aactatctga taaagacatt tcctaaagaa aagattgcgg ttgccattgc tcatgatagc
    11701 agaaacaata gccggagttt tgcccaaaat gccgctgacg tgctctctgc caatggcatt
    11761 actgtgtatt tatttgatga gctccgccct acccctgagc tatcatttgc tgtaaggaca
    11821 ctggaatgca aaagtgggat tgttataacc gcatcgcata accccaaaga atacaacggg
    11881 tataaagtgt actgggctga tggcggacag ataatatcac cacacgacaa aaacattatt
    11941 gcggaagtaa gaaacataag cagaatttcg gaggtaaaat tcacgggaaa ctccgcattg
    12001 atacatccca ttggtagtga tatcgataca ttgtatatcg aaaaagtaaa atccttgagt
    12061 ttgaatcctg aagtaattca gcggcagcag aagctttcga tagtgtacac acccattcac
    12121 ggttcagggg tgaacattgt tccaatggct ttacaaagtt tcggtttcac caatattatt
    12181 cgtgtccctg agcaggatat ccctgatggt aatttcccta cggtgcattc gcccaaccct
    12241 gaggaagcgg ctgctctgag catggctttg gaatatgcaa aaaaggcagg ctccgaaatt
    12301 gttatgggta ctgaccctga tgccgacagg gttgggataa tagtaaggga ttcaaacggt
    12361 gaatatgtgt tgctgaatgg caatcaaacc gcatctgttt tactgtatta cctcatcagt
    12421 gaatggcacc gcaaaggaaa aattgatggc agacagttta ttgttaaaac cattgttact
    12481 accgacttgt tggctgaaat tgccgactct ttcaatgtaa agaactatga tgtactcact
    12541 ggctttaaat acattgctgc tatcatccgc gaatacgaag ggaaaatgga atttatcggt
    12601 ggcggagaag aaagctacgg gtatctggca ggtgattttg tgagagacaa agatgcggta
    12661 atttcctgtg ccctgattgc tgaagttgct gcctgggcaa aagataacgg aaaaactatc
    12721 attgacatac tgaaagaaat atatgtacga ttcggatttt ataaagaaaa aatgatatcg
    12781 gtaacacgta ccggaaagtc gggagcagaa gaaattgctg ccatgatgga ttcgttccgg
    12841 aatcagcctc ccgaatcgat atgcggaact gatgttacca ttattcacga ttacgaaaaa
    12901 caagctagca tcgatatgct gagtcaactg cgctacacaa ttcccctgcc caaatcggat
    12961 gtgcttcagt ttgaactgaa agacggttca aaaattacag taagaccttc gggcacggaa
    13021 ccaaagatca aattttattt cagcttaaaa gcatcgctcc agcgggttga agattacgat
    13081 acggtgaatg tagaacttga ccagcttgtc gaaaaaatgg caaaagaaat cattcgttaa
    13141 atgaagtcgt accgaaaaga gctctggttt cgtaccgaaa cacggcgtaa actgataaat
    13201 attacaccta agataattca atgccttact gaaagcggaa tacgcgaggg gttctgcctg
    13261 gtgaacgcaa tgcatattac ggccagtgta tttataaacg atgatgaagc cgggcttcac
    13321 cacgattttg aggtgtggct cgagaagctt gctcctgaaa aaccccattc acaatatcgc
    13381 cacaacggtt acgaagacaa tgccgatgcg cacctaaagc gttcagtaat ggggcgcgaa
    13441 gtaataattg gagttaccga aggtgagctt gactttggcc cctgggaaca gattttctat
    13501 ggagaattcg atggcatgcg cgacaagcgc gtgttggtga aaattatcgg ggaatagcag
    13561 cacctacttt tttgtatcga ggtacttcag gtaacggaca atttcctttg gttttacatc
    13621 tttggcaact attattttgt tgctgtcgag aataaacaag cgtggagtgc gtgtggttcc
    13681 gtaatagatg aaaaaatcgt cggtactgcc ggcactgcaa gcatttaccc acgataattt
    13741 catgtttgta acatagtctt cccatgcatt tttatcggac tgtatgttga tggcaataag
    13801 ctctgctccc atctttttaa tttcttcgta gctactgtgg agcaccggag tttgttcatc
    13861 gcagagtgag cagccccgct cccaaaacca cagaattata tagcgcgcct gaatgctgtc
    13921 agtttcgaaa agagctccgt cgattcgttt caaatgcagg ttatgcgact ttgcacccag
    13981 catattgtac ttcatttcgt caactctgct gcgtatcatg tcgattgctt tttcgtcggc
    14041 ccatttaatt ttaccgttca ggaagtagtt gtttgccaga taaacaaata ctgcatccat
    14101 acctattacc gaagggtttt cgaaatgatc gacaagatat gatacaatat atttttcgaa
    14161 atcgatgttc accgatgctt tggcaatgag agcatcaaca tctttataca cattttcgat
    14221 actatcgaca cgggtactca ggaagaattt tgaatattcg tcgagcatgc ggtgaaaaac
    14281 gaattcaggt gttcgcaaca gcctttcgtc actaaaatcc actgcatcga aataatgatc
    14341 tttataataa tagtaatacc ttgcagggtc ctcggtctgc cattgcaata catacggcgg
    14401 aaattcaggc ggaagcattg ctgccatcat tttcgaaaga aaaccattgt tgtcctgagt
    14461 tttgtaatat ttgtacacaa ccattctcct gtttttcaaa ctgtcttcgg tgcgttttat
    14521 ttcatctgca ttagcctgac cagaatttgc ataatactgt ttttttatat cgagctcaga
    14581 gcttgccaga ttgatggcag caatgtcgcg ctgaaggcca aagaaaaggt cgggtttttc
    14641 ggtacctgtc ataataagtt tccctagtac attatcgaga ccggtggtaa ttgaaaaatc
    14701 ctgatcgtcg agaacaagaa aatcgaatcg tgttttaggg ggtattacaa tgaaatacac
    14761 cccttgcggt aacacaaaat cgccgaaaaa aaccgcatga cccttactat cggtctgaat
    14821 ggtatcaatc acctgatatt caggaccgta gtgcgatcca aaatataaaa gtccattttc
    14881 gtgcccggta acagtaactt cgattcggaa ggca
//
LOCUS       DAOOQP010000075        14871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4924_length_14871_cov_5.389511, whole genome shotgun sequence.
ACCESSION   DAOOQP010000075 DAOOQP010000000
VERSION     DAOOQP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14871
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4924_length_14871_cov_5.389511"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLK75_08530"
     CDS             complement(<1..990)
                     /locus_tag="PLK75_08530"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOY39332.1"
                     /translation="MKPFFSNISYKFKVIIPLIISIVLSIVVVTLFSVQKSRENIRKS
                     MENYLSLEVQTVKKMFEREKALKTEKAITGMKVAHTIFYSNTLTITGNDYTIEAENQL
                     TGTKHDEELQTWFLDGESLNGGSAFVDTVQYLLGGTVTVFQKMDSGFVRIATNVLKTD
                     GSRATGTFIPFGSPVVEAVMKGETYVGRAFVVNDWYASVYEPIVVNNEIAGMLYVGNK
                     EKDISELSVALKSLKIGTSGYVYVFDEDGNVLIHPENEGKNFARETFFGEITKNKKGL
                     IRYPFEGNMRLSAYDYFDDFKLYIVATINEKEETKQQINNIILSSSVITFLIIL"
     gene            complement(1230..2249)
                     /gene="pheS"
                     /locus_tag="PLK75_08535"
     CDS             complement(1230..2249)
                     /gene="pheS"
                     /locus_tag="PLK75_08535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOY39333.1"
                     /translation="MKDIIGRLITEIEAYLPENAVENENFRLKYLSKKGAISILFDDF
                     RLLAPDEKREVGKMLNELKNLASSKFETFKELFEKQQTSTNNIDVTLPGEPGEIGSRH
                     PVSLLISDICSVFTRIGYTLSHGPEIEDDDHNFARLNFAPDHPARDMQDTFFITPDLN
                     ILLRTHTSSVQAHEMQEMKPPIRTISPGRVYRNEAVSARSHCQFHQVEGLYIDENVSF
                     ADLKQTLLYFAKEMFGEQTRIRLRPSFFPFTEPSAEMDVSCTLCGGKGCRVCKNTGWL
                     EVLGCGMVDPNVFDAAGIDSQKYTGFAFGIGVERMAMLKFGVNDIRLYFENDLRFLKQ
                     FKTIY"
     gene            complement(2268..3167)
                     /locus_tag="PLK75_08540"
     CDS             complement(2268..3167)
                     /locus_tag="PLK75_08540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY39334.1"
                     /translation="MSKRIIYISLILAVLSTSCSLLNKTGNMKNYVLFKTDMGDLVVG
                     VYEATPMHRDHFIKLCNKHYYDSLLVFRASPSGYIATGDSLSRTATATTLLGDNITDT
                     AINPEFNSKLINRKGAVGFLRADDKINPRKMSHSGIIYFVIGEVFDDKMLTKLETQTN
                     APVIGKYVSAFMDEPGHEKLRDSMQYYRQHRLMDDFRRIYVQVTDSVMPQIKKDKVSL
                     FSLSPYQRKAYTTEGGLPTMDNNYTVFGHIVFGFNVIEQMSKVRTGIRFRPRTDIYIH
                     STTVMNKKEWKNFLKEHKKLANQ"
     gene            3240..3653
                     /locus_tag="PLK75_08545"
     CDS             3240..3653
                     /locus_tag="PLK75_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39335.1"
                     /translation="MNKTIYAVILLSLFAGLFVFLNSCKEPNPPEAIVIVQDEDGQPV
                     EGAMVIVKAASSDSSHTVVYLLSGPKLVADTSWTNENGQVSYEFKYQSIYKVEVTKGT
                     DRDHPFVRRGLGVLMLENDKTYVETITINEQTVFN"
     gene            3684..4844
                     /locus_tag="PLK75_08550"
     CDS             3684..4844
                     /locus_tag="PLK75_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39336.1"
                     /translation="MMKRFFLFLNFIILVYVLSAQIVYKRSDFASVNDVYPVARYSNS
                     SPTSGMLVSSFGEIANLLYEDFTFEQCVLDTIRYYAPDEYDPDDYYPLANCAFINEIG
                     YVVFLRITYPQVEVVGMQGEIPMLGGTANLKSEQNLFTLRFPLTTGLDLSESGSFSQQ
                     FHISMFADIIPTEYYDLVVALYDSVKFSITLEQISDFDTEGFLTINDTNSFNDARNVL
                     REYRYEKQITNVYLHAIYNGQWTPLQDAPLIGDQLPVELPMVDSTYTINYWTKYLGIP
                     MVSMKTSPDYLTAYSVALFDGEFTGISPVDSELWMVYPNPANEMIYFKNLKQTPAHIR
                     IFSIDGKLVADDDFNSDIFIFDAQNTNQGMYFYELWNEDELNKTSGKFLIVK"
     gene            4856..4929
                     /locus_tag="PLK75_08555"
     tRNA            4856..4929
                     /locus_tag="PLK75_08555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4890..4892,aa:Ala,seq:ggc)
     gene            4984..6165
                     /locus_tag="PLK75_08560"
     CDS             4984..6165
                     /locus_tag="PLK75_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY39337.1"
                     /translation="MDTSKKGFNTKLIHAGDHEDVYGSAVTPIYQTSTFKFRSAKHGA
                     DCFAGKDPGYIYTRIGNPTINSLEDKINALENGVGCIALSSGMAAVSTVYMALLGKDA
                     HMIGTSAIYGPSRGVMERDFSRFGVSFDFIDTSDITNIEKHIRPNTKLLYLETPANPT
                     MQITDIRKAAELAHQHNILVCVDNTFCSPYLQRPLDLGADIVLHSLTKFINGHADIVG
                     GALVAANHEIYKKLRQTMVYIGGNMDPHQAYMVYRGLKTLSLRMDRSQQSACIIAEYL
                     EKHPKVEWVKYPGLKSHPQFELASSQMDGPGAMISFELKGGLKAGEILMDNVHIATLA
                     VSLGGVETLIQHPASMTHAGVSRENRIGAGITDGLVRYSVGIEDVNDLVEDLDQAFKK
                     V"
     gene            6203..7042
                     /locus_tag="PLK75_08565"
     CDS             6203..7042
                     /locus_tag="PLK75_08565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY39338.1"
                     /translation="MAKQKRSFKKWLNKKFRLVLYLSDSLEARWTFRFSRAGMVLLNF
                     TILALLMAVFWLIVVHTSIKESIPGYPDSNVKEYMITNKIRIDSLEEELRMRDQYLEV
                     IKSILVGIDSVDSNYMFPAGNMGNDSLSRVKSELTKGGIWVNQSFKNPFPKQDVINLY
                     PPVKGMVSNSFNAAMQHFGTDIVAIDDKSVKAARSGTVISANFTVETGYSVVIQHDMG
                     IVTIYRHNSKIMVKAGDKVVAGQVIAAMGNSGELTNGPHLHFEVWIDGKPVNPEDYTR
                     FTK"
     gene            7039..8205
                     /locus_tag="PLK75_08570"
     CDS             7039..8205
                     /locus_tag="PLK75_08570"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOY39339.1"
                     /translation="MSNKRIAILGSTGSIGTQAIDVIVNNPEKFTAEVLVANTNSDLL
                     IQQARMLEPNMVVIGDESKYTTVKEALSDIPVKVFAGKDSIRHAAAIGSVDLVLTAMV
                     GFAGLEPCIQALEAGKVIALANKETLVVAGDLITRLSKMYQAPIIPVDSEHSAIFQCI
                     IGEVSNSVEKLILTASGGPFRGKNRESLQDVTPEQALKHPNWTMGAKVTIDSASFMNK
                     GLELIEAHWLFGLSPDEMEVIIHPQSIIHSMVQFCDGSVKAQLSPPDMRMPIHYAFAF
                     PERVPSSFGRINFDSSLNLTFEKPDLVVFRNLAIAYEALRKGGNVPCAMNAANEVAVQ
                     AFLQRKTGFLQMSDIIEKTLEQTYFISSPDLTDYIETDKAARIIANELINKNKL"
     gene            8202..9503
                     /gene="rseP"
                     /locus_tag="PLK75_08575"
     CDS             8202..9503
                     /gene="rseP"
                     /locus_tag="PLK75_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOY39340.1"
                     /translation="MNGIIMAAQLILGLAILVLVHEFGHYIAARIFKIRVDKFFIFFD
                     AGFKIWSKKIGDTEFGIGWLPFGGYCKIAGMIDESLDKEQMKQPPQPWEFRSKPAWQR
                     LIVMLAGVIMNVILGILIFTMSHLVFTKEYLPAENVKDGIFAYEQARFIGFKTGDHIV
                     AINGKEVERAEDILSTRFYFGGEITVKRNNDFVVVDVPDTLYKYISRGNPFIGFDNFP
                     LNIDSVNDTLNAWKAGLRNGDKLVSLDNIPVSSYGNFKEILFKHRGDTLDFEIDRAGS
                     SMFLCIPVDSMGKIGVYARMPEYQTKKYTVFTAFKYAYSDAFEAIYSNIKGYKMIFTG
                     VEKPKDIAGPIGIAKVYGAEWNWARFWYITGLLSMVLAFLNILPIPALDGGHALFLVI
                     EAVTRRKFSDRFIEIVQYIGLAILLVLMVFLLGNDIVNLFR"
     gene            9506..10315
                     /locus_tag="PLK75_08580"
     CDS             9506..10315
                     /locus_tag="PLK75_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39341.1"
                     /translation="MIRKWIILCFALFPSTLFAQLELDFRTGPVHDVRMLIVPFDPII
                     YYNDATPEMAQKYNMTHDQLMTYFRREWNKQIHMAFADSCYTVDLYSDNTKEARDDLD
                     GLYTIISYEMRNKMQNAPEEEKELSLFRKIFPKKEKQQSDENFYGTKKQNGELVNYTQ
                     GTENKFFHIVFNDTTFLRTLAERRDIDYFLFINQFEIKGNYSDPYASGRSDFTRTLKV
                     HFSIFDRTGTLVHGSYASVDIPFYLYDKDIVVGEYFPPAIRQIIHNVKFSY"
     gene            10318..10893
                     /locus_tag="PLK75_08585"
     CDS             10318..10893
                     /locus_tag="PLK75_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOY39342.1"
                     /translation="MELIFATNNPHKLREVSEMLPPHIIIKSLADIQCFEDIPETGST
                     LSENAVQKAKYVSDKYHYDCFADDTGLEVEALNMQPGVFSARYAGNQKNPADNIEKLL
                     LQMQGVTNRKARFRTVIALSLQGKILLFEGTVNGSIIHTLKGEKGFGYDPVFVPDGYT
                     HSFAEMSPQTKNKISHRALAFEKLIGFLSTF"
     gene            11019..13367
                     /locus_tag="PLK75_08590"
     CDS             11019..13367
                     /locus_tag="PLK75_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY39343.1"
                     /translation="MNTIQKYFSIIPLLIISMLAVGQVAVGQWRDHLPYNYGKMLAET
                     DSKVFVVTNVGMYSYDKNSGEIEKLSKITGLSDAGMLSVAYDSTHDLVFIGYSNGNID
                     IINNNRIINVPDIKRKPLTYSKAINSILFIDDYAYLSCGFGIVVFDMVKLEIKDTYYI
                     GDNGGFVYVNEIATDGDNLYAATVQGIYKGAFTSNLVDFSNWQVITDMYAIDRFAWVA
                     GMSFNCLEWFGGKLIVNFDNQEEANSDTLLVFDNGSWSYLDTVVKATNSITSNGNKLV
                     VTSDYYIRMYNEGLENYHHVWSYNFENGSLYPNYAIIGKENTLWIADRLYGLVRSRNL
                     WTFEKKSINGPLNYVFFDMASAGNEVYGVAGGFNLSLTPTYRYSMLYRFSDQWDTRYY
                     VNEPEMQPVTDLVSVAVNPANPTQVYAGSWTKGLLEFNNFALSNIFDETNSPIETIPG
                     NDLIRVGGLAFDSENNLWITCSDNEMPVVVKMADGTWHTVDYSAEINALNVRQIIITQ
                     TGKKWIVLPRGGGLFVFDDHGTPADKSDDEYRKLSIVDEYGQVISNEVFSIAEDKNGA
                     VWVGTNAGVVVYYNPDDVFDQSVFYGQQVKIPRNDGTDDADILLGAETVTAIAVDGAN
                     RKWFGTQNGGVFLTSSDGISQKYHFSTDNSPLLSNNVYCITINPSSGEVFFGTERGLI
                     SFRGEATEGDNDFTEVYSFPNPVPPGYTGTITIHGLIAGSYVKITDISGNLVTEMRSL
                     GGQAVWDGNDINGNRVCSGVYLVFSSNEDGSKKNITKILFMN"
     gene            13378..13971
                     /locus_tag="PLK75_08595"
     CDS             13378..13971
                     /locus_tag="PLK75_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HOY39344.1"
                     /translation="MIRNLLLFALTLLLTANTLSAQSDFYNRLADSAYVLTQQKVRYD
                     PKYMQIPYPNGDVPSNIGVCTDLIVRAYRKFGIDLQKLVHEDMQSNFNLYPKYWGLSK
                     PDSNIDHRRVPNLMVFFERYGSVKKNTENSIDYKPGDIVCWRLNSWSTHIGIVSNRKS
                     ADGLRYMMIHNIGEGQVLEDCLFSWTIIGHYQYGSKQ"
     gene            14080..14808
                     /gene="recO"
                     /locus_tag="PLK75_08600"
     CDS             14080..14808
                     /gene="recO"
                     /locus_tag="PLK75_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOY39345.1"
                     /translation="MIVKTRAIVLKKIPYGESSFVVHLFTEALGRQSVIVRGVRNPKS
                     KVKAALFQPMTVLNCEFNFKESQSLHSLKEVRVDFPFQQIYSRIEKNAVVMFMCEILS
                     KTLREEGPQPDLFLFLRQILIHLDDENTQVANLPLFFLLRLLPYLGFKPGDNFSSQTP
                     YFNIREGVFMPVFTTETDTVNKDATVILWLLMQADHRNYQAVKMPKSTREYLVQKIAD
                     YYAIHVNNFGSLKTIPVLHDVLNS"
BASE COUNT         4409 a         2828 c         3186 g         4448 t
ORIGIN      
        1 aaggattatc agaaaagtga taaccgacga agaaagaata atattgttga tctgttgttt
       61 tgtttcttct ttttcgttaa tggtcgccac aatatacagt ttgaaatcgt cgaaataatc
      121 gtaagccgat aagcgcatgt ttccttcgaa aggatagcgg atcaatcctt ttttattttt
      181 cgtaatttca ccgaaaaagg tctctctcgc aaagtttttc ccctcgttct cagggtgtat
      241 cagcacattc ccatcttcgt caaatacata aacgtagccc gatgttccaa ttttgaggct
      301 tttgagtgca acacttaatt cacttatatc tttttcttta ttcccaacat acagcatacc
      361 tgctatctcg ttgttcacaa cgatgggttc atatactgaa gcataccagt cgttcaccac
      421 aaaagccctg cccacatagg tttcgccttt cattacagct tcaactacag gcgacccgaa
      481 ggggataaat gttccggttg cccttgaacc atcggttttt agtacattgg ttgcaattct
      541 cacaaaaccg gaatccattt tctgaaaaac ggtaaccgtt cctcctaaaa ggtattgcac
      601 tgtatcgaca aatgcgcttc ctccgttcag tgattcccca tccaaaaacc atgtctgcag
      661 ttcttcatca tgtttggtgc ctgtaagctg gttttctgct tcaatagtat agtcatttcc
      721 ggtgatagtt aaggtattgg aataaaaaat ggtatgagcc actttcattc cggtaatggc
      781 tttttcggtc ttcaaagcct tctctctttc aaacattttc tttacggttt gaacttcaag
      841 acttaaatag ttctccatcg acttacggat attttcgcgc gatttctgaa ccgagaacaa
      901 ggtaacaacc actatggaca gaacaatgct tattatcagc ggaataataa ctttgaactt
      961 atacgaaata ttcgaaaaaa atggcttcat aaaaatacct ccaggacatc ttcattgaaa
     1021 tttatgcaaa aataccatat ttgtaaatgc aaaaacagat gcctgagcaa gaatttgggg
     1081 aagatgagcg aatgtggttt aacagttaaa aatgtcaaat gttaaaagtc agaagtcaaa
     1141 tgtaaaatgt tttatgtaat ttgaggaaac cataaacatt taacatcaga catttgacgt
     1201 cagacattaa aaatacgata ttcttttgtt taatagattg ttttaaattg tttcaggaac
     1261 cgaaggtcgt tttcgaaata caatctaatg tcgttcactc caaatttaag cattgccatt
     1321 cgttcaacgc cgatgccgaa tgcaaacccg gtgtatttct gcgaatcgat acctgcagcg
     1381 tcaaacacat tggggtcaac cattccacag cccagtactt cgagccatcc cgtgttctta
     1441 caaacacgac atcccttgcc tccgcatagg gtgcagctta catccatttc ggctgaaggt
     1501 tcggtaaagg ggaaaaacga agggcgcagg cgtatacggg tttgctcgcc aaacatttct
     1561 ttggcaaaat acagcagcgt ttgtttgaga tcggcaaacg atacattctc atctatatat
     1621 agcccttcaa cctgatgaaa ctggcagtgc gaacgtgccg acacggcttc gttacgatac
     1681 accctgcccg gagagatagt acggataggt ggtttcatct cctgcatctc atgtgcctga
     1741 actgaggagg tatgcgttcg aagcagaatg ttcaggtcgg gagtgataaa aaacgtgtcc
     1801 tgcatgtcgc gtgccggatg gtcgggcgca aaattaagac gggcgaaatt atgatcatca
     1861 tcttcaattt caggaccatg actcaatgtg tagccaatac gggtaaaaac actgcagata
     1921 tcgcttatca atagcgacac cgggtggcgt gatccgattt cgccgggttc acccggaagt
     1981 gttacgtcga tattgttcgt tgaggtttgc tgcttttcga acagttcttt aaaggtttcg
     2041 aatttcgatg aggcaaggtt cttcaattcg ttcagcattt taccaacctc ccttttttcg
     2101 tcgggagcca gcaatctgaa atcgtcgaat aatatgctga tcgcaccctt tttagaaagg
     2161 tatttcaacc ggaaattttc attttcaact gcattttcag gtaaataagc ttcaatttca
     2221 gttatcagcc tgccgataat atctttcatt gataatagta ttgagtgtta ctgattggct
     2281 agttttttat gttcttttaa aaagttcttc cattcctttt tattcattac cgttgtggaa
     2341 tgaatgtaaa tatctgttct gggccgaaac ctgatgccgg ttctcacttt cgacatttgc
     2401 tctattacat tgaatccgaa aacaatatgt ccaaacacag tataattgtt atccatggtg
     2461 ggcaaacctc cctcagtagt ataagctttc cgttgatagg gcgacaacga aaacagggag
     2521 actttgtctt ttttaatttg tggcataaca ctatcggtaa cctgtacgta aattcttcgg
     2581 aaatcatcca tcaaacgatg ttgccggtaa tattgcatac tgtcacgcaa cttctcatga
     2641 ccaggttcat ccataaaggc actcacatat tttccgataa cgggtgcatt ggtttgggtc
     2701 tcaagttttg taagcatttt atcgtcgaaa acttcgccta tcacaaaata aatgataccc
     2761 gagtgcgaca tttttcgggg atttattttg tcgtcggcac gcagaaagcc aacggcacct
     2821 ttgcggttaa ttagtttgct gttgaattcg gggtttattg cagtgtcggt aatgttatcg
     2881 ccaagcaaag tggtggcggt ggcagtgcgc gataaactgt cgccagtggc aatatagcct
     2941 gaagggcttg ccctgaaaac gagcaaacta tcgtaataat gcttgttgca aagttttata
     3001 aaatgatcgc ggtgcatggg tgtagcttcg taaactccca ctacaagatc gcccatatcg
     3061 gttttgaaca aaacatagtt tttcatattg ccggtcttat tcagaaggct gcacgacgtg
     3121 gacagaactg ctagaatcag gcttatgtaa ataatacgtt ttgacattac aataaaattt
     3181 tgaaataaat aaaatatatt gtataattgt gggttgcaaa gttataaaat atatttacaa
     3241 tgaataaaac tatctacgct gttattttgc tgagcctgtt tgcgggtttg tttgtattcc
     3301 ttaattcttg caaagaacca aatcctcccg aagcaatagt tattgttcag gatgaagatg
     3361 gtcagccggt ggaaggtgct atggttatcg ttaaagctgc gtcttctgac agttctcaca
     3421 ccgttgttta cctgctcagt ggtcccaaat tggttgccga tacgtcgtgg actaatgaaa
     3481 acggtcaggt atcatatgag tttaaatacc agtcgattta taaagtagag gttacaaaag
     3541 gcaccgatcg cgatcatcct tttgttagaa gaggtcttgg tgttctcatg ctcgaaaacg
     3601 ataaaaccta cgttgaaaca attactatca acgagcagac tgtatttaat tagtatatcc
     3661 tttaaaataa tcaaaccttt attatgatga aaagattttt tctttttttg aattttataa
     3721 tattagtgta tgtgctatca gctcagattg tttacaaacg ctctgatttt gcaagtgtaa
     3781 atgatgttta tccggtagcc aggtactcaa attcatcgcc aacctccgga atgctggtaa
     3841 gtagtttcgg ggaaattgcc aatttgcttt acgaagattt tacttttgag caatgcgttc
     3901 ttgatacaat taggtattac gcacctgatg aatacgaccc tgatgattat tacccactgg
     3961 caaattgtgc ttttattaac gaaattggct atgtggtttt tttgcggatt acataccctc
     4021 aggtggaagt tgttggaatg cagggagaaa ttcccatgct tggaggcacc gcaaacctta
     4081 agtcggagca gaaccttttt acattgaggt tcccgcttac aacagggctc gatttgtctg
     4141 aatccggctc gttttctcag caattccata tttcaatgtt tgccgatatt atccctactg
     4201 agtattatga cctcgtagtt gctctctacg actcggttaa gttcagtatt acccttgagc
     4261 agatttccga ttttgacaca gaaggatttc tcacaattaa tgacacaaac tcatttaatg
     4321 atgccagaaa tgtgttacga gaatatcgtt acgaaaagca aataaccaat gtatatcttc
     4381 atgcaattta caatggtcaa tggacccctc tacaggatgc accacttatc ggagaccaac
     4441 tgcctgttga attgcccatg gtagattcta cttacacaat taactactgg acaaaatacc
     4501 ttggtattcc aatggtgtcg atgaaaacca gccctgatta tcttacagcc tattctgttg
     4561 ctctgttcga tggagaattt actggaatca gtcctgttga tagcgaattg tggatggtgt
     4621 acccgaatcc cgcaaacgaa atgatttatt ttaaaaatct taaacaaacc ccggcacata
     4681 ttcgaatctt tagtatagat gggaaattgg tggcagatga tgattttaat tcagatatat
     4741 ttatctttga cgcacaaaat accaatcagg gaatgtactt ctatgagctt tggaatgagg
     4801 atgaactcaa caaaaccagc gggaagtttc tgattgtcaa atagttcttt taataggggc
     4861 attagctcag ttggctagag cgcgtgactg gcagtcacga ggtcagcggt tcgaccccgc
     4921 tatgctccac aattttcaag gagttcttgt tgagctcctt ttgtttttta ataatttatg
     4981 gttatggata catcaaaaaa aggattcaac acaaagctga ttcacgcagg tgatcacgaa
     5041 gatgtgtatg gtagtgcagt tactccgatt tatcagacat caacatttaa attccgtagt
     5101 gcaaagcatg gtgccgattg ttttgccgga aaagaccctg gttatattta tacccggatt
     5161 ggaaatccaa cgattaattc gcttgaggat aaaataaatg cccttgaaaa cggtgtcgga
     5221 tgcattgctc tttcatcagg gatggctgca gtatcaacag tttacatggc attgttgggg
     5281 aaggatgcac acatgattgg taccagtgct atttatggtc ccagccgtgg agtaatggaa
     5341 cgggattttt cgcgcttcgg tgttagtttc gattttatcg atacttccga cattacaaat
     5401 atcgaaaagc acattcggcc caatacaaaa cttttgtatt tagaaactcc tgcaaaccct
     5461 acaatgcaga ttaccgacat acgtaaagca gccgaactgg cgcatcagca taatatcctg
     5521 gtctgtgtag ataatacttt ttgctcacct tacttgcagc gtccacttga tttgggtgcc
     5581 gatatagtgc ttcattcgct taccaaattt ataaatggtc atgccgatat agttggtggt
     5641 gcattggtgg ctgcaaatca tgagatatac aaaaaacttc gtcagaccat ggtttatatt
     5701 ggtggcaata tggatccgca tcaggcatac atggtatatc ggggtttaaa aacactatca
     5761 ctccgtatgg atcgttctca gcaatcggca tgtattattg cagaatatct ggagaagcac
     5821 ccaaaggttg agtgggtgaa atacccaggg ctgaaatcgc atcctcagtt cgaactggca
     5881 tcttcacaaa tggatggtcc gggtgctatg attagctttg aactcaaagg ggggcttaaa
     5941 gctggagaaa tacttatgga taatgtccat attgcaacac tggcggtttc gctaggcggt
     6001 gtcgaaacac tgatacaaca ccctgcgtcg atgacccatg ccggagtgag tcgcgagaac
     6061 cgaattggtg ctggtatcac cgatggtctg gtgaggtatt ccgttggtat tgaagatgta
     6121 aatgacctag tcgaagattt ggatcaggct tttaaaaaag tataattgca aaataaatta
     6181 cttcgtttta cgatattaat gcatggcgaa gcaaaaacgc agttttaaaa aatggctgaa
     6241 taaaaaattc aggcttgtat tgtacttgtc cgacagcctc gaagcccggt ggacattcag
     6301 gtttagccgt gcaggtatgg ttttactgaa ctttacaata ttggctttac tcatggcagt
     6361 tttctggctg attgttgttc atacgtcaat caaggaaagc atccctggat accccgacag
     6421 taatgtaaaa gagtacatga ttacaaataa aatccgaatc gatagccttg aagaggagct
     6481 aaggatgcgc gatcagtacc tcgaagtaat taaaagtata ctggtgggaa tcgattcggt
     6541 tgacagcaac tatatgtttc cggcaggaaa tatgggaaac gacagtctgt ccagagtgaa
     6601 atcggaactg acaaagggtg gtatttgggt aaatcaatcg tttaaaaatc ctttccccaa
     6661 acaagatgtc atcaatcttt atccccccgt taaaggtatg gtatccaact cttttaacgc
     6721 tgcgatgcag cactttggta ccgatattgt agctatcgat gacaaatcgg tcaaagcagc
     6781 gcggtcggga acggtaatct ctgcaaactt cactgttgaa accggatata gtgttgttat
     6841 tcagcacgat atggggatag taacgattta cagacacaat agtaaaatca tggtgaaagc
     6901 tggtgataaa gtggtcgctg gtcaggtaat cgcagctatg ggcaattcag gagaacttac
     6961 caatggcccc catttgcatt ttgaagtctg gatcgacggg aaaccagtca atccggaaga
     7021 ttatacacga tttacaaaat gagcaataaa cgtattgcca tattaggttc aacgggctcc
     7081 atcggaactc aggctattga tgttatcgtg aataaccccg aaaaatttac ggctgaagtg
     7141 ctggttgcaa acacgaatag tgatctgctg attcagcagg ccagaatgct ggagcccaac
     7201 atggtagtta ttggcgatga atcgaaatac accacggtga aagaggccct ttctgatatt
     7261 cctgttaagg ttttcgccgg taaagattcg attcgtcatg ctgcggctat tgggtctgtc
     7321 gaccttgttt taacggcaat ggttggtttc gccggactgg aaccctgcat tcaggctctt
     7381 gaggcaggga aggtgattgc acttgccaac aaggaaaccc ttgtggtggc aggtgacctc
     7441 attactcgac tatcgaaaat gtatcaagca ccaataatac ctgtcgattc cgaacactct
     7501 gcaattttcc agtgtattat cggagaggta tcaaattctg tcgaaaaact aattttaaca
     7561 gcatcaggag gtccgttcag aggaaaaaac agagaatctc tccaagatgt tacccctgaa
     7621 caggctttaa agcatcccaa ctggacgatg ggcgcaaaag ttacaatcga ttcggctagt
     7681 tttatgaaca aagggctgga actcattgaa gcccactggc tttttggctt aagtcctgat
     7741 gaaatggaag taattataca cccgcagtca ataattcatt ccatggttca attctgcgat
     7801 ggttcagtga aggctcagtt aagccctccc gacatgagaa tgcctattca ctatgctttt
     7861 gcatttcccg aaagagttcc ctcatcattt gggcgcatca acttcgacag ctcacttaac
     7921 ctgacttttg aaaaacctga cctggtggta tttcgcaact tggcaattgc ctacgaggca
     7981 ttgagaaaag gtgggaatgt cccatgtgct atgaatgctg ccaacgaagt tgctgtacaa
     8041 gcgtttctgc aacgtaaaac cggctttttg caaatgtcag atattattga aaaaactttg
     8101 gaacaaacct attttataag cagccccgat ttaactgatt acatcgaaac cgataaggct
     8161 gcgcgcatta ttgcaaatga attaattaat aaaaacaagt tatgaatgga ataataatgg
     8221 ctgcccagtt gattttaggg cttgcaatac tggttctggt gcacgaattc ggacattata
     8281 ttgctgcgag gatttttaaa atacgggtag ataaattttt tatttttttc gacgcagggt
     8341 ttaagatttg gagtaagaaa atcggtgata ccgaatttgg tattgggtgg ctgccttttg
     8401 gcggatattg caaaatagcc ggaatgatag atgagtcgct cgacaaagag cagatgaaac
     8461 aacctcccca accctgggaa ttccggtcaa aaccagcatg gcagcgattg attgtaatgc
     8521 tcgcaggtgt tattatgaat gttattctgg gcattctgat ttttaccatg tcgcacttgg
     8581 tttttacgaa ggaatatctc cctgccgaaa atgtgaagga cggaattttt gcgtatgagc
     8641 aagcccgatt cattggtttt aaaacagggg atcatattgt tgctatcaat ggaaaagaag
     8701 ttgaacgtgc cgaggatata ctctctacac gtttttattt cggaggcgaa attaccgtga
     8761 agcgaaataa tgatttcgtt gttgttgacg ttcccgatac attatataaa tatatcagca
     8821 gaggaaatcc cttcattggt ttcgataatt tccctttgaa tatcgatagt gtaaacgata
     8881 ctttaaatgc atggaaagcc ggtcttagaa atggagataa acttgtttca cttgataata
     8941 ttccggtaag ctcatatggt aattttaaag agatattgtt taaacacagg ggcgataccc
     9001 ttgattttga aatagatagg gctggtagct caatgtttct atgcattccc gttgattcga
     9061 tgggtaaaat aggtgtttat gccagaatgc cagaatatca aacaaaaaaa tatactgtat
     9121 ttactgcatt caaatatgct tattccgatg ctttcgaggc catatattcc aatataaaag
     9181 ggtataaaat gatatttacc ggtgtggaaa aaccaaaaga tattgccggc ccgattggaa
     9241 tcgccaaagt gtatggagct gaatggaatt gggctcggtt ttggtatatt accggtttgc
     9301 tttcaatggt acttgctttt ttaaatatcc tgccaatacc tgctttagat ggaggccatg
     9361 cattatttct ggtaattgaa gctgtaactc ggcgaaaatt ttctgacagg tttattgaaa
     9421 ttgtacaata tattggcttg gcaatactct tggtactaat ggtattttta cttggtaacg
     9481 atattgtaaa tttattccga taactatgat tcgtaagtgg ataatactgt gttttgcact
     9541 tttcccttca acactttttg cccagctgga actcgatttc cggacaggcc ctgtgcatga
     9601 tgtaagaatg cttatcgtgc cattcgaccc cattatatat tataatgatg ccactccaga
     9661 aatggcgcag aaatataata tgacgcatga tcagctgatg acatatttcc gtcgcgaatg
     9721 gaacaagcag attcatatgg ccttcgccga ttcgtgctat acagtcgatt tatattccga
     9781 taatacaaaa gaagccagag acgatttgga tggactatat accatcatca gttacgagat
     9841 gcgtaataaa atgcagaacg cccctgaaga ggagaaagag ctatccttat tcagaaaaat
     9901 atttcccaaa aaggaaaaac aacaatcaga tgaaaatttt tatggtacaa aaaaacagaa
     9961 tggtgaatta gtaaattata cgcagggtac cgaaaataag ttttttcaca ttgtattcaa
    10021 cgacaccact tttttgagaa cactggccga aaggcgcgat atcgattatt ttttgtttat
    10081 aaaccagttc gaaattaaag gaaattatag cgacccgtat gcttcgggaa gatccgattt
    10141 taccagaaca cttaaggtac atttcagtat ttttgacaga acagggactt tggttcatgg
    10201 aagctatgct tctgttgata taccatttta tctttacgat aaagatattg ttgtcggcga
    10261 atatttccct ccagcgataa ggcaaattat tcataacgtc aaattttcgt attgacaatg
    10321 gagctgatat ttgccacaaa caatcctcac aaactcagag aggttagcga aatgctgccc
    10381 cctcacataa tcattaaaag ccttgccgat attcaatgct ttgaagatat tcctgaaacc
    10441 ggttctaccc taagtgagaa tgctgtgcaa aaagcgaaat atgtttctga taaataccat
    10501 tacgattgct ttgccgacga cacaggcctt gaagtagaag ccctaaacat gcaaccgggc
    10561 gttttctcag cccgatatgc cggtaatcag aaaaatcctg ctgataatat tgagaaatta
    10621 cttttacaaa tgcagggagt tacgaatcgg aaagctcggt tccgaacggt aatagctctg
    10681 agtttacagg gtaaaatttt gctttttgaa ggcacggtga acggaagcat aattcacaca
    10741 ttgaagggag aaaaaggctt tggttatgat cctgtttttg tgccagacgg atacactcat
    10801 agctttgccg aaatgtcgcc ccaaacgaaa aacaaaatca gccacagggc tttagcattt
    10861 gaaaaactaa tcggattcct ctccacattt tgatcaatat aataattcgc tgtcatttca
    10921 gttataattt ttattagtat tgtatgatga aagcagggct gaagaatcca atacaggagt
    10981 aagttttaaa gtttaatgtg ataattgttg caattaatat gaatactatt caaaaatact
    11041 tttcgattat accgttgctc attatttcaa tgctggcggt cggacaggta gcagttgggc
    11101 agtggagaga ccatttacct tacaattacg ggaaaatgct tgccgaaacc gatagtaagg
    11161 tctttgttgt tactaatgta ggaatgtata gctacgataa aaactccggc gaaatagaga
    11221 aactctcgaa aattacagga ctttccgacg cagggatgct ttcggtagcg tacgatagta
    11281 cccatgacct tgtgtttata ggttatagta atggaaatat tgacattata aataacaata
    11341 gaattataaa tgttcctgat attaagcgaa aaccacttac atattcaaaa gcgatcaata
    11401 gtattttgtt tattgacgac tatgcatacc tgtcgtgcgg gtttggtatt gttgttttcg
    11461 atatggtaaa acttgaaatt aaagatacat attatattgg cgataatggt ggtttcgttt
    11521 atgtgaacga aattgccact gatggcgata atctgtatgc agcaacggtc cagggtattt
    11581 ataagggggc atttactagc aatcttgtcg atttttccaa ctggcaagta attactgata
    11641 tgtatgcaat tgaccggttt gcctgggtgg cggggatgag ttttaattgt ctggagtggt
    11701 ttggtgggaa gctgattgta aacttcgaca accaggaaga agccaattcc gatacgttgt
    11761 tggtttttga taatgggagc tggtcgtatc tcgacacagt agtaaaagct accaactcaa
    11821 ttacgtcaaa tggcaataaa ctggttgtta caagcgacta ttatatcagg atgtacaacg
    11881 aaggtcttga gaattatcat catgtatggt cctataattt cgaaaacgga tccctatatc
    11941 cgaactatgc aattatcggc aaggaaaaca cgctttggat tgccgaccgt ttgtatggac
    12001 tggtaaggag tcgtaacctc tggactttcg aaaagaaatc gattaacgga cccttaaact
    12061 atgttttctt cgatatggct tctgcgggaa acgaagtgta tggtgttgcg ggaggtttta
    12121 acttgtcgct cacacccaca tatcgatata gtatgctgta tcgtttttcc gatcagtggg
    12181 acacaagata ttacgttaat gaacctgaaa tgcaaccggt taccgacctt gttagtgttg
    12241 ctgttaatcc ggcgaaccca actcaagtgt atgcagggtc gtggacgaaa ggactgctgg
    12301 aattcaataa ttttgcacta tcaaatattt ttgatgaaac gaatagtcca attgagacca
    12361 tccccggaaa cgacctgatc agggtcgggg ggctggcatt cgattccgaa aacaaccttt
    12421 ggataacatg ctccgacaac gaaatgcctg tggtggtaaa aatggccgat ggtacatggc
    12481 atactgttga ttacagtgcc gaaattaacg cacttaatgt acggcagata attattaccc
    12541 aaacagggaa aaaatggatt gtacttcccc gtggaggagg cctttttgta tttgacgatc
    12601 acgggacacc tgccgataaa agtgatgatg aatatcgcaa acttagtatt gttgatgaat
    12661 acgggcaggt tataagcaac gaagtgtttt caatagcgga ggataaaaat ggagcagttt
    12721 gggtaggaac aaatgcaggt gttgtagtgt actacaatcc ggatgatgtt ttcgaccagt
    12781 cggtttttta cggacagcag gttaagattc cgcgaaacga tggtaccgat gatgctgata
    12841 ttctgcttgg agcagaaacg gttacggcaa ttgcagtgga tggagcaaac cggaaatggt
    12901 tcggcacaca gaatggaggg gtgtttctta cgagcagcga tggtatctct cagaaatacc
    12961 atttcagtac agataatagc ccactgttat cgaataatgt gtactgtatc acaattaacc
    13021 caagcagcgg agaggtgttt ttcggtacag aacgcggtct tatttctttt aggggagaag
    13081 ccaccgaagg ggataacgat tttactgaag tatattcgtt cccgaaccca gttccacctg
    13141 gttatacggg aacaatcact attcacggtt tgattgccgg ttcttacgtt aaaattaccg
    13201 atatttcggg aaaccttgtt accgaaatgc gatctctggg aggtcaggct gtttgggacg
    13261 gcaacgatat taacggaaat cgtgtgtgct ctggagttta ccttgttttt tcatcaaacg
    13321 aagacggatc aaaaaagaat attacaaaaa tattattcat gaattaaggg cttacgaatg
    13381 ataagaaacc tgctcttatt tgctctgaca ttattgctga cggcaaacac gctttccgca
    13441 caatctgatt tttataaccg actggctgac tctgcctacg ttttgaccca acaaaaagtt
    13501 cgttacgatc ctaagtatat gcaaatccct taccctaacg gtgatgttcc gtcaaatatt
    13561 ggagtatgca ccgatttaat agtgcgagca tacagaaaat ttggaattga tttgcaaaaa
    13621 ctggtacacg aagacatgca gtcaaatttt aatctatatc ccaaatattg gggattaagc
    13681 aagcccgaca gcaatattga tcacagacgt gttccgaacc tgatggtgtt ttttgaacga
    13741 tatggctcgg taaagaaaaa taccgaaaat tcaatcgatt ataaacccgg cgatattgtc
    13801 tgttggagat taaacagctg gagtacacat attggtattg tttcgaaccg aaaatcagca
    13861 gacgggctaa gatacatgat gatacataat attggagaag gtcaggttct tgaagactgc
    13921 ttgttcagtt ggacaattat cgggcattat caatacggta gtaagcaatg atttatttgc
    13981 ataaggcgag tatgcgaatc gactgttttt tagaatgggt ttacgaagaa gctgttattt
    14041 tggacattga gaagtagcaa agcggaataa taaaaaaaga tgattgttaa gacccgggcc
    14101 attgtattaa agaaaatccc ttacggggaa agcagttttg ttgttcacct ttttaccgaa
    14161 gcattagggc ggcaatctgt tatcgtacgg ggtgtgagaa acccaaaatc aaaggtaaaa
    14221 gcggcccttt ttcaaccaat gaccgtattg aactgtgagt ttaattttaa agaatcgcag
    14281 tcgttgcata gccttaaaga ggtgcgggtt gatttccctt ttcagcagat atattcacga
    14341 atcgagaaaa atgccgtggt tatgtttatg tgtgaaattc tatccaaaac cctaagagaa
    14401 gaagggcctc aacctgattt gtttttgttt ctgcgccaga ttctcatcca tctcgatgat
    14461 gaaaataccc aggttgcaaa tttgccatta ttcttcttac ttcgtttact accctatctg
    14521 ggttttaaac caggtgataa tttctcatct caaacacctt atttcaacat cagagaagga
    14581 gtgtttatgc cggtctttac caccgagaca gatacggtga acaaagatgc gacagttatt
    14641 ctatggctat tgatgcaggc tgatcataga aattatcaag cggtaaaaat gccaaaatca
    14701 acccgggaat atctggttca aaaaattgca gactattatg ccattcatgt gaataatttt
    14761 ggttccctga aaactattcc cgtattgcac gatgttttga attcatgaat tcaaaatagt
    14821 agtggtgcgt taatttttta tttttagtca taactcattg atattaatat a
//
LOCUS       DAOOQP010000076        14863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4928_length_14863_cov_5.244665, whole genome shotgun sequence.
ACCESSION   DAOOQP010000076 DAOOQP010000000
VERSION     DAOOQP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_14863_cov_5.244665"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /gene="ftsA"
                     /locus_tag="PLK75_08605"
     CDS             <1..1111
                     /gene="ftsA"
                     /locus_tag="PLK75_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOY39346.1"
                     /translation="PDHEIVSADIEFLRKKVNEVSVETGEEILHVIPQSYIVDREVGI
                     KNPVGMCGRQLEGNFHVVIGQMAAISNIRKCVNRLAIKVNDLILEPLASSSAVLTCDE
                     KEIGVAMVDIGGGTTDLAIYHDGMIVHTAVIPFGGNAITSDIRQYFNILERQAEELKK
                     QYGSAIYEKSQEEIIITIPGLKGRPPKELSLSKLTMITKARVEEIIHAVMFQIETSGV
                     ADKLGAGIVICGGGALLKNIRQAFSYITELDVRVGYPNEYLNSDVVEEINQPQFATAV
                     GLIMKGYELQNTVVAKKEPFDLIKDTFPGDSQPDEKEMRKLKEKERKEREKAEKEEKK
                     KAKLDDDDDDKEKTSFMDKIKTTVSKLFDEDDNKF"
     gene            1131..2447
                     /gene="ftsZ"
                     /locus_tag="PLK75_08610"
     CDS             1131..2447
                     /gene="ftsZ"
                     /locus_tag="PLK75_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOY39347.1"
                     /translation="MLDIDFGIEKQSTSIIKVIGVGGGGSNAVNQMYRLGICDVDFII
                     CNTDAQALKSSPVATKIQLGKSLTEGLGAGNNPERGKQAAIESLKDIEDALDNNTKMV
                     FITAGMGGGTGTGAAPIIAKAAKEREILTVGIVSVPFKVEGPLRKDQAINGIKELQQY
                     VDSLLIINNERLQEIFKDWKMSGAFSKADEVLATAAKGIAEIITVHGYINVDFEDVRT
                     VMKNSGVALMGSAVAEGENRALLAIKEALNSPLLKDNDIKGAKNILLNIMSGKGDNEI
                     SMSEFGAITTYVTQAVGTSSNIIWGTGFDETLDTQLRVTVIATGFNSFPDFYNGEPKK
                     VIHIPLGEEIPVQTQAGDLEFAQDEILEIDESDDESARTVEFVINNKPETSLHDTEAD
                     IDDKSQLRDHEDFGHLKDLKKLNESSYLDNIENEPAYKRLNKNEDN"
     gene            2472..2918
                     /locus_tag="PLK75_08615"
     CDS             2472..2918
                     /locus_tag="PLK75_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOY39348.1"
                     /translation="MTIPEQINEDIKKAMLARDKVRLTALRSVKSAFLLVNTEAGDKG
                     LTDERALSIIQKMVKQRRETAEIYKTQNRPDLCENEIAEADAIAEYLPKQMSTVEIEE
                     VVKVVIQQVQATGIKDMGKVMGTVNKQLAGKADGRVVSEIVKKLLS"
     gene            complement(2923..3630)
                     /locus_tag="PLK75_08620"
     CDS             complement(2923..3630)
                     /locus_tag="PLK75_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY39349.1"
                     /translation="MEQLQDNDPRLKVFGDNASIFFSLSEEEKNSLIINTSIRQIKKN
                     EIVFNEGARPSGLICLSQGKVKIFKEGVGGREQIVRMAKPGGFIGYRALFAGQNYHAS
                     AVAIENSVACTIEKEALFNVMKQNPGLTFRVLKSLATELGISNSQTVNLTQKHIRGRL
                     AESLLFLAETYGYEEDGVTLKAYLSREDVANLSNMTTSNAIRTLSTFAGEKVIDLDGR
                     KIKILNISKLQRISDLG"
     gene            3786..5606
                     /locus_tag="PLK75_08625"
     CDS             3786..5606
                     /locus_tag="PLK75_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39350.1"
                     /translation="MKKFSFLLMVLFVATFAFGQNLMLQKSRSIPVKSDKADQTKDLQ
                     VIWENDFSTPAEWTMEYSNDNPNDGPWVIGTDAPAGYYSEGMGAIESTTAANGFAMYD
                     SDAIGNNASGTQDSKITYTGTINCTGYTNVGVQFESYYRKFQGTPYLEISLDGTTWTP
                     YEVHSDLGVNAATDNPAYVVVNISTVAANQPAVHIRFRYLGVWDYAWMVDDVKVYVAP
                     DYDVSLIQARINFFPQYINYGYSGFYGRIPAFQIDGIGAPVFASGIIKNYGTQAATPN
                     LNFTVLDHTNTEVYTSSVTFDAALGTEEFDTASTGEPYYYFDPATYGMYTFNLDADVT
                     GVTDENPENNVLSYNTYLTPATWAHDNDNLTGQWSTGNYTSGGIDGDIVGVTYPFFVS
                     CDVNSVDVYIGSMTELGTSFVVKLMTWDEGSSAWVEAIGSEIITLENDDQVGAMHTIT
                     FPDTYTVEVPEGELVEILVAVEIYFGGAEFRLGVDATAPTSGWETWLYFSGEDTWYYF
                     GGDYVPIIRMNVGPYSATNTLTSDEINMYPNPTRNLVNFTNVEGANIQIVDLTGKVVA
                     NIDAAQNEISFDLSSYAQGTYIVRITNEDGLTIKKVNLVK"
     gene            complement(5700..6032)
                     /locus_tag="PLK75_08630"
     CDS             complement(5700..6032)
                     /locus_tag="PLK75_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOY39351.1"
                     /translation="MISINKVQLIGEISCSPECCDANGNKIARFTLLTIDYYQGSGKK
                     FFDRQWHKVVAIGKQAIITTKYLKKGDEVVIDGSITKRIYVDKNGRSTIVTEIIAHDI
                     IYPFTKRA"
     gene            complement(6177..7958)
                     /gene="argS"
                     /locus_tag="PLK75_08635"
     CDS             complement(6177..7958)
                     /gene="argS"
                     /locus_tag="PLK75_08635"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOY39352.1"
                     /translation="MEQILKAKTIEAVKAIYNKTALDNDIQIQFTNKEIEGDYTIVVF
                     PLLQYSKNKPENTAKELGVYLKNKISDISSYNVIKGFLNIRMTTEYWIEKLNKISFGE
                     EKPDKNSTPLRYMVEFSSPNTNKPLHLGHIRNNLIGDSVSRILRAAGSEVIRVNLVND
                     RGIHICKSMLAYNKWGNNCSPESTGIKGDKFVGDYYVKFEQEYKKEIAKLVTEGSDEK
                     DAALQSTLMKEAREMLQQWEKGDMDTMNLWRKMNNWVYEGFEETYKSLGISFERTYYE
                     SQTYTSGKEIVFEGLEKGLFNKKDDGSIWVDLTDYGLDEKLLLRSDGTSVYITQDIGT
                     ALQRFSEFNLDKHIYVVGNEQNYHFQVLKLVLKKLGFDWADNIYHMSYGMVELPEGKM
                     KSREGTVVDADDLINEMLQTATSMSSEHGKLNEVDTDYKIKALKMIALGALKYFILKV
                     DPKKTITFNPRESIDFNGNTGPFIQYTYARICSILRNAEKMNIKFNGKASEETNTVNK
                     EIELLRLIDKYKYVLREAARNFDPGIIANYCFEIAKEFNQYYQEYSILKEKDINILHF
                     RLLLCKLIKETMHNGMNLLGIEMPERM"
     gene            complement(8022..8675)
                     /gene="nth"
                     /locus_tag="PLK75_08640"
     CDS             complement(8022..8675)
                     /gene="nth"
                     /locus_tag="PLK75_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOY39353.1"
                     /translation="MTKKERYQGVISWFSNNIPVAETELHYTNPYQLIVAVILSAQCT
                     DKRVNIITPAFFKHFPTVYSLSEAKIDEIYELIKSCSYPNNKTNHLKRMAETLVNDFN
                     GIVPSDTAELIKLPGVGRKTANVIASVAFNQPKMAVDTHVFRVSNRLGLTRNAKTPLA
                     VETELVKHFPESLIGKAHHWLILHGRYVCKARKPQCESCGISLYCKFFEHLQKNVNN"
     gene            complement(8688..9608)
                     /gene="trxB"
                     /locus_tag="PLK75_08645"
     CDS             complement(8688..9608)
                     /gene="trxB"
                     /locus_tag="PLK75_08645"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOY39354.1"
                     /translation="MEKTKCLIIGSGPAGCTAAIYASRANLNPIMYEGLEPGGQLTTT
                     TEIENFPGYPEGITGPLMMEDLKKQAQRFGADIRYGIVTKVDFSKRPFTVTIDGSSSI
                     EADTVIIATGASAKYLGIESETRFKGQGVSACATCDGFFYRGKDVAVVGGGDTAAEEA
                     TYLAGLCHKVYLIVRRDVLRASKAMQERVMRTPNIEVLWKHQTKEIVGNMSGVTGAIL
                     TDNNGQEKHIDIHGFFVAIGHHPNSDVFRSFIETDETGYIKTIPGTTKTNVPGVFACG
                     DVQDKHYRQAITAAGSGCMAAIDAERFLSE"
     gene            complement(9617..10117)
                     /locus_tag="PLK75_08650"
     CDS             complement(9617..10117)
                     /locus_tag="PLK75_08650"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOY39355.1"
                     /translation="MKKLLIILILLGNMSILLAQNSTPVFWSFSVRESNDTSFVLHIK
                     ATIEKGWYIYSEDVTDGGPIPLIINFDETTNLIAGGTLTAKTASVELYDDIFEMNIRY
                     YEDSAIFERQFIPSGNRVDLLFYSEAQACFKKDGQCRLESNDILIQAKKEDNIWIIEN
                     TKIIIK"
     gene            complement(10114..11043)
                     /locus_tag="PLK75_08655"
     CDS             complement(10114..11043)
                     /locus_tag="PLK75_08655"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOY39356.1"
                     /translation="MKIRIIHITLILTVILALFSCETDISVELPRPEEKLVVEGYIAS
                     GEHPVVFLTKNSGYFDSITAETVIDMIVLDSTVVTVSDGSVTDTMSLSFYPGKFPYFG
                     YSCSDIIGEYGKEYRLDIYWKNNHYFATTRIPQPVAIDSVWFTLLTDYDSLGILSFRY
                     QDPPERGNYYAYHTRVEGEQTEFYKPYYGSHIDDDLYDNGAQVIYFGITRGMDEISFF
                     ETEQSDEEFLNSLAFGVGTNVSVRLSTIDIEHYEFWNSYYRHMITWGNPFTNPATIKS
                     NIEGDPALGIWGGYGTSIAKVHITDSATIEMLR"
     gene            complement(11045..13420)
                     /locus_tag="PLK75_08660"
     CDS             complement(11045..13420)
                     /locus_tag="PLK75_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39357.1"
                     /translation="MKYYLLLLIIIFTAAASYSQKFTISGYVKDASSGEELIGANVFV
                     ESLKQGATTNTYGFYSLSLPAGSYIVTYSFIGYADEKKEINLNHSQSFNVSLKSTAYT
                     TDEVVVSGERTDRNIQDSRMSVNKLPVEEVKTIPAFMGEVDVLKTIQLLPGVQSAGDA
                     NSGFYVRGGGPDQNLILLDEAVVYNAAHLFGFFSVFNADAIKDVELYKGGMPAQYGGR
                     VSSVLDITMDNGNMKSYHFDGGIGTISARITAQGPIIKDTCSFLISARRTYIDILIKP
                     FVNDTSPFKGSGYYFYDFNAKFNYRFSDKDRLFLSGYYGKDVFTFKSNETPFTMKIPW
                     GNGMVSLRWNHLFNDRFFLNTTTVFSDYQFSTDINMKNNDGTDGFRLIQNSGIRDFNL
                     KQDYTWLPNTKHTIKFGMNYVYHIFTPNTVNADFGDMEYNFNRKNQQFAHEAAVYIGD
                     DYIITDKISLYAGFRFAGFFQTGPFTRYIKDQNNLQTVDSIMYGSNQLVCDYYSPEPR
                     FSIKISTGETSSFKASFMQNNQFIHLASLSASTLPTDLWVPSSDRVKPQFGRQYAMGY
                     FRNFYNNQYEASLEIYYKDLRNLIEYMDGAMYGDDLNDNPDNYFIFGDGNSYGLELFV
                     KRRTGRVTGWVGYTWSKTTRFFEGVDNGEPFPAKYDRRHDLSVTASYQLSEKWSFGGV
                     FVFATGNTTTLPIARYIINGQIVNEYGPRNSYRLAPYHRLDVSVTYTHKKTDKWKSEW
                     NLSVYNVYNRKNPYFIYFYYEGNLDTESFRTVAKQVSLIPILPAITWNFSF"
     gene            13569..13642
                     /locus_tag="PLK75_08665"
     tRNA            13569..13642
                     /locus_tag="PLK75_08665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13603..13605,aa:Pro,seq:ggg)
     gene            13713..13970
                     /locus_tag="PLK75_08670"
     CDS             13713..13970
                     /locus_tag="PLK75_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOY39358.1"
                     /translation="MGYYVYVLESQSSKSLYVGQTANPTSRLREHNSGRSKYTRNKGP
                     WEMIFLKDFPSRIEAVNFEKKLKALKSPGCIRKLIISGSVG"
     assembly_gap    14104..14203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14245..14610)
                     /locus_tag="PLK75_08675"
     CDS             complement(14245..14610)
                     /locus_tag="PLK75_08675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOY39359.1"
                     /translation="MKILRSFKYAINGLRIVVTEERNAVIHTIITGAVILSAILLKIN
                     HYEWIAIIFAIGFVFVSEIINTVIENLADFVTKENNIDIKRIKDIAAAAVLVSAIVAA
                     IIGLIVFLPRILMLFSFNS"
     gene            complement(14664..>14863)
                     /locus_tag="PLK75_08680"
     CDS             complement(14664..>14863)
                     /locus_tag="PLK75_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOY39360.1"
                     /translation="IYTDDFMEVWNKGFRDFRNRKIFKQGMCKNCKEWNHCMGDGMHL
                     HKPSTPDPVICYYHKLYQKNH"
BASE COUNT         4346 a         2963 c         2971 g         4483 t
ORIGIN      
        1 ccccgaccac gaaatagtca gcgcagacat agaatttctt cgaaaaaaag taaatgaggt
       61 ttccgtggaa accggcgaag aaattctgca cgtaattcca cagtcatata ttgtcgacag
      121 agaggttgga attaagaacc ctgtgggcat gtgtggccgg caacttgagg gaaattttca
      181 tgtggtaatc ggacaaatgg ctgcaatctc gaatatacgt aaatgtgtaa accgtcttgc
      241 aataaaggtg aatgatctca ttctggaacc tcttgcttcg tcatcggcag ttttaacttg
      301 cgatgaaaaa gaaattggtg tggctatggt tgatataggt ggtggaacaa ccgaccttgc
      361 tatctaccac gacggaatga ttgtacacac tgccgtaata ccatttgggg ggaatgccat
      421 aacttctgat atcagacaat attttaatat tctggaacgg caggctgaag agcttaaaaa
      481 gcaatatggt tcggccatat acgaaaagtc gcaggaagaa attatcatca caattccggg
      541 tttaaagggc cgccctccaa aggaattgtc gctgagcaaa cttacaatga ttactaaagc
      601 gcgggtcgaa gaaataatac acgcggtaat gtttcagatt gaaacctctg gcgttgctga
      661 caaattaggc gccggaattg taatttgcgg aggcggtgct ttgttgaaaa acatcaggca
      721 ggcattctcc tatataactg aattagatgt gcgcgttggt tacccgaacg aatatctgaa
      781 cagcgatgtg gtggaagaga ttaaccaacc tcagtttgca actgctgttg gtcttataat
      841 gaaaggctac gagttgcaga atactgttgt ggctaaaaaa gagcctttcg atttaattaa
      901 agatactttt cccggagata gtcagcctga tgaaaaagaa atgaggaaac tcaaggagaa
      961 agagcgtaaa gaacgtgaaa aagccgagaa agaagaaaag aaaaaagcca agctcgatga
     1021 cgatgacgac gataaggaaa aaacaagttt catggacaag ataaaaacca cggtttcgaa
     1081 actttttgat gaggacgata acaaatttta ataattaaac ctaacaaaat atgctggaca
     1141 ttgatttcgg aattgaaaaa cagagcactt caataattaa agtgattgga gtgggtggcg
     1201 gtggcagtaa cgctgtcaat caaatgtacc gtctgggtat atgcgatgtt gacttcatta
     1261 tctgtaacac cgatgcacaa gctcttaaaa gcagccctgt agctacaaaa attcagctgg
     1321 gcaaatcgct gaccgaaggc ttgggagcag gtaataatcc ggagcggggc aaacaggcag
     1381 ccatcgaaag cctcaaagat attgaagacg cactcgacaa caatacaaaa atggtattta
     1441 ttacagcagg tatgggaggt ggcaccggaa ccggtgctgc accaattata gccaaggctg
     1501 caaaagaacg ggaaatactt accgtgggca ttgtttcagt gcctttcaaa gtggaaggac
     1561 cgctgcgtaa ggatcaggca ataaacggaa ttaaggagct tcagcaatat gtcgattcgt
     1621 tgctgattat aaataacgaa cgcctgcagg aaatatttaa agattggaaa atgtcgggag
     1681 cattcagcaa agccgacgaa gttctcgcaa ctgctgctaa aggtattgcc gaaataatca
     1741 cggttcatgg atatatcaac gttgacttcg aagatgttag aaccgtaatg aaaaacagtg
     1801 gggtagcact tatgggctca gctgttgccg aaggagaaaa tcgtgcactc ttagccatta
     1861 aagaagcttt aaattcacca ctgttgaaag ataatgatat taagggggca aagaacatcc
     1921 tgcttaatat tatgagtggc aaaggcgaca atgaaatttc gatgagtgag ttcggagcta
     1981 ttactactta tgtaactcag gctgtcggaa cttcttcgaa tattatttgg ggtacaggtt
     2041 tcgacgaaac cctcgataca caactcagag ttactgttat agcaacaggg tttaattcat
     2101 ttcccgattt ttacaacggt gaaccgaaga aagtgattca cattcctctg ggagaagaaa
     2161 tccctgttca gactcaggcc ggtgaccttg agtttgctca ggacgaaatt ctggaaattg
     2221 atgaaagcga cgacgaatcg gccagaacag tggagtttgt gattaataat aaaccggaaa
     2281 catcgttaca tgataccgaa gcagacattg acgataaatc gcaacttcgc gaccatgaag
     2341 attttgggca ccttaaagat cttaaaaaac tcaatgaatc atcttacctc gataatatcg
     2401 aaaatgaacc tgcctacaaa cgattaaaca agaatgaaga taactgattc gacaaaatat
     2461 aactaactgc tatgactatt ccggaacaaa ttaatgaaga tattaaaaaa gcaatgcttg
     2521 caagagacaa agtcagactt accgctttgc gatctgtaaa atccgctttt ttgcttgtaa
     2581 ataccgaagc cggcgacaaa ggcctgactg atgaaagagc actttctatt atacagaaaa
     2641 tggtaaaaca acgccgcgaa actgctgaaa tttataaaac acaaaaccgg cctgacctat
     2701 gcgaaaacga aattgccgag gctgatgcaa ttgctgaata tctgcccaaa caaatgtcaa
     2761 ctgtcgaaat tgaggaagtt gtgaaagtgg tcattcaaca ggttcaggcc accggtatca
     2821 aagatatggg taaagtaatg ggaacagtta ataaacaact tgccggaaaa gccgatggaa
     2881 gggtggtttc cgaaattgta aaaaaactgc tttcctgaaa tactaaccca ggtcagaaat
     2941 acgttgtagt ttgctgatat tcagtatttt aattttcctg ccgtcaagat caataacttt
     3001 ctcaccggca aaagttgaaa gtgttctgat agcattactg gtggtcatgt tgctcaggtt
     3061 agctacatct tccctagaaa gatatgcttt cagggtaact ccatcttctt cgtagccata
     3121 agtttctgca aggaaaagca acgattcagc aaggcgcccg cgtatgtgct tttgggttag
     3181 atttactgtt tgagagttgc tgatacccaa ttctgtggca aggctcttga gcacacggaa
     3241 tgtaagtccc gggttttgct tcatcacatt aaacagtgct tccttttcga tggtgcaggc
     3301 aacggagttt tcgatagcta cagccgaagc atgatagttc tggccggcaa acagagccct
     3361 gtaaccaata aatccaccgg gtttggccat acgtacaatt tgctccctgc caccaactcc
     3421 ttccttaaaa attttaacct taccctgcga cagacatatc agcccacttg gccgtgcccc
     3481 ttcgttaaaa acgatttcat tcttttttat ctgcctgata cttgtgttta taatcagtga
     3541 gtttttttct tcttccgaaa gactgaaaaa aattgaggca ttatcgccaa acacttttaa
     3601 ccttggatcg ttatcctgta gttgttccat tacttattca actttggtga cttggcaaag
     3661 gtaaataatt caaattgttt tgacaaatta agtagcgcag ctgggtatta attttttttt
     3721 tattgttgtt attttttttg taaatttggc gttagttata attttataaa tttaaagtaa
     3781 tagttatgaa aaaattttct tttttattaa tggtactttt tgttgctacc tttgcttttg
     3841 ggcagaacct gatgctccag aagagcaggt cgattcctgt taaaagcgac aaagcagatc
     3901 agaccaaaga tcttcaggta atttgggaga acgacttttc aactcccgca gaatggacaa
     3961 tggaatattc aaacgacaac cccaatgatg gtccctgggt aattggaact gatgctcctg
     4021 ctgggtatta ttccgaaggt atgggtgcca tcgaatctac aaccgctgcc aatggttttg
     4081 cgatgtacga ttctgatgca attggtaata atgcatcggg aacccaggat tcaaaaatta
     4141 cttacaccgg tactatcaat tgcaccggct ataccaacgt aggtgttcag tttgaatcat
     4201 attaccgtaa attccagggt actccctatc tggaaatcag ccttgacgga actacatgga
     4261 ctccttatga agttcacagt gatttgggcg taaatgctgc aaccgataat cctgcttacg
     4321 tagttgtaaa tatttctact gttgcagcaa atcaacctgc agttcatatc cgcttcagat
     4381 atcttggagt gtgggattat gcatggatgg ttgacgatgt taaggtgtat gttgctcccg
     4441 actacgatgt aagcctgatt caggcacgta ttaatttctt cccgcaatat atcaattacg
     4501 gatatagcgg attctatgga cgcattcctg ctttccagat tgatggtatc ggtgcccctg
     4561 tttttgcaag tggtatcatt aagaactatg gaacacaagc tgcaactccg aacctcaatt
     4621 tcacagtgtt agaccatact aataccgaag tttacaccag ctcagttact ttcgatgctg
     4681 ctttaggtac cgaagaattc gatacagctt caacaggtga accgtattac tatttcgatc
     4741 ctgcaactta cggaatgtat acttttaacc tggatgcaga tgttacgggt gttaccgacg
     4801 aaaaccccga gaataacgtt ctttcttaca atacttattt aacaccggca acatgggccc
     4861 atgataacga caatcttaca ggtcagtgga gcacaggcaa ctacacttcg ggtggtatcg
     4921 acggcgatat cgttggtgta acttatcctt tcttcgttag ctgcgatgtg aactctgttg
     4981 atgtatatat tggttcaatg accgaacttg gaaccagttt tgttgtaaaa cttatgacat
     5041 gggacgaagg ttccagtgct tgggttgaag caatcggaag cgaaattatt accctggaaa
     5101 acgacgatca ggttggtgca atgcacacca ttactttccc cgatacttat accgttgaag
     5161 ttcccgaagg cgaactggta gaaattcttg ttgcagttga aatttatttc ggtggcgctg
     5221 aattcagact tggcgtagat gctactgctc caacatccgg ttgggaaaca tggttatatt
     5281 tctcaggtga agacacatgg tattattttg gcggcgacta tgttccaatc attcgtatga
     5341 atgttggacc ttacagtgca accaatactt taaccagcga tgaaatcaac atgtatccga
     5401 acccgacacg caatttagtt aacttcacca atgtagaagg cgcaaacatt cagattgttg
     5461 acctcactgg taaggttgtt gcaaacatcg atgctgctca gaacgaaata tcgttcgact
     5521 tgtcatcgta tgctcagggt acctacattg ttcgtattac taacgaagac ggacttacaa
     5581 ttaaaaaagt taatcttgta aaataagtct gacttacggt ttacctaaaa aggctgcagc
     5641 aatgcggcct tttttatttc tgatgctaac cgacaatacc ttttcaaagc gagactatgt
     5701 tatgctcttt tagtaaaagg gtagataatg tcgtgtgcaa ttatttcagt tacaatggtg
     5761 gaacgtccat ttttatcaac ataaattctc tttgttatgc taccatcaat tactacttca
     5821 tcgccttttt ttaagtattt tgtagtgatg atagcctgtt ttcctatggc aacaacctta
     5881 tgccattgcc ggtcgaagaa ttttttcccg ctgccctgat aataatcaat tgtaagaagg
     5941 gtaaacctag ctattttatt cccgttagca tcgcaacatt caggggagca actgatttca
     6001 ccaattagct gtactttatt aatcgaaatc ataattgaaa ttttcagcaa atatcgtaga
     6061 caattgcaaa tcaatcgtat gatagctgtt tactttcggt ttttattcgt ttgtaaacgg
     6121 atgtaaatgg aaagttgaaa accaagaatt gtacaaatga accctgaaac gatacattac
     6181 attctttcag gcatctcaat ccccagcaaa ttcatgccgt tgtgcatggt ttctttgatt
     6241 aacttgcaaa ggagtagcct gaaatgtaaa atgttgatgt ctttttcttt taaaatggaa
     6301 tactcctgat aatactgatt gaactctttt gcaatttcga agcagtaatt tgcaatgatg
     6361 cccgggtcga aattcctggc agcttcccgc agaacatatt tatatttgtc gataagccgg
     6421 agcaattcta tttctttgtt aaccgtattg gtttcttccg aagcttttcc gttgaatttg
     6481 atattcattt tttcggcgtt acgcaatatg gagcagatac gggcataagt gtactgtata
     6541 aatggcccgg tattaccatt aaaatcaatc gattcacgtg gattaaaggt aatggttttc
     6601 tttgggtcaa ctttaagaat aaaatatttg agtgccccta aggcaatcat tttcagtgct
     6661 tttatcttgt aatcggtgtc gacttcattc agtttgccat gctccgacga catcgatgtt
     6721 gcggtttgta gcatttcgtt tatcaaatcg tcagcgtcaa ctacggtacc ttcgcgcgat
     6781 ttcattttac cttcaggcaa ttccaccatt ccgtacgaca tgtggtaaat gttgtctgcc
     6841 caatcgaatc caagcttttt caacacaagt ttaagtacct gaaaatgata attctgctca
     6901 ttaccaacaa cgtaaatatg cttatcaagg ttaaattcgc tgaagcgttg cagcgcagta
     6961 cctatgtcct gagttatata cacactggtt ccatcagaac gaagaagtag tttctcgtca
     7021 agtccgtaat cagttaaatc gacccatata gaaccatcgt cttttttatt gaaaagacct
     7081 ttttccagtc cttcgaaaac aatttcttta cctgaagtgt aggtttgcga ttcgtaataa
     7141 gttctttcga agctaatccc aagtgattta taagtttctt caaacccttc gtaaacccag
     7201 ttattcattt ttcgccacag gttcatagta tccatatcgc ctttttccca ctgctgaagc
     7261 atttctcgtg cttccttcat taaggtgctt tgcagagcgg cgtctttctc gtcggagcct
     7321 tcggttacca gtttggcaat ctctttcttg tactcctgtt caaatttcac ataataatca
     7381 ccgacaaatt tatcaccctt aattccggtt gattcaggcg agcaattatt accccattta
     7441 ttataggcca gcatcgattt gcaaatatga atacccctgt cgttgacgag gttaacgcgt
     7501 attacctcac ttccggcagc tctcagaatt ctggaaacag aatccccgat caggttgtta
     7561 cggatatgac ccagatgtag tggtttattg gtattcggtg aagaaaactc aaccatataa
     7621 cgcaaaggtg ttgaattctt atcgggcttt tcttctccga acgatatttt gttaagtttt
     7681 tctatccaat attccgttgt cattcggata tttaaaaatc ctttgataac attatatgac
     7741 gagatatctg aaatcttgtt tttcaggtaa accccaagtt ctttggctgt gttttcaggt
     7801 ttgtttttgc tgtattgcag tagtggaaaa acaacgatag tataatcacc ttcaatttcc
     7861 ttgtttgtaa actgaatctg aatgtcgtta tcaagtgctg ttttattata aatagccttg
     7921 actgcttcta ttgtttttgc ttttaatatt tgttccatga aaaccttact aaagtgcagc
     7981 aaatatatta aaatgcgcta tactttactt ttatgattct tttaattatt aacatttttt
     8041 tgcaagtgtt caaagaactt gcaataaaga gaaatgccac agctttcgca ctggggcttg
     8101 cgggctttac atacataacg gccatgaaga ataagccaat gatgagcttt acctatcaat
     8161 gattcaggaa aatgtttcac tagctctgtt tcaacggcaa gtggtgtctt tgcatttcgg
     8221 gttaggccaa gtctgttcga caccctgaaa acatgggtat cgactgccat tttgggttga
     8281 ttaaatgcta ccgaagcaat tacatttgct gtttttcgac cgactccggg tagtttaatt
     8341 aattcggctg tatcagatgg aacaataccg ttaaaatcat tgacgagggt ttccgccatt
     8401 cttttcaggt gatttgtttt attgtttggg taagaacacg attttattaa ttcataaatt
     8461 tcatcgattt tggcttctga taggctataa accgtaggaa aatgtttgaa gaacgctggt
     8521 gtaattatat taacacgttt gtcggtgcac tgtgccgata gtattacagc cactatcagc
     8581 tgataagggt ttgtatagtg cagctcggtt tcggcaacag gtatattgtt gctaaaccat
     8641 gaaataacac cctgatagcg ctcctttttt gtcatatcag tactgcttta ctcgctgaga
     8701 aagcgttcag catcaattgc tgccatacat cctgacccgg ctgcggtaat agcctggcga
     8761 tagtgtttat cttgcacatc gccgcaggca aatacacctg gaacattggt tttggtggtt
     8821 ccgggaatgg ttttaatata tccggtttca tcagtttcga taaacgacct gaatacatca
     8881 ctgttagggt gatggccgat agccacaaaa aaaccgtgga tatcgatatg cttttcctgc
     8941 ccgttattat cggtaagtat tgctccagta acccccgaca tatttccaac aatttctttt
     9001 gtttgatgtt tccacaaaac ctcgatgtta ggtgttcgca taaccctctc ctgcattgct
     9061 tttgatgccc ttaatacatc gcggcgcaca ataaggtaaa ctttgtggca aagccctgca
     9121 agatatgtgg cttcttccgc agctgtgtcg ccaccgccaa caacggcaac gtctttgcct
     9181 ctgtagaaaa aaccatcgca ggttgcacat gccgatactc cctgaccttt gaatcgtgtt
     9241 tctgactcta ttccgagata tttagccgat gcgccagtgg ctataattac cgtatctgct
     9301 tcaatgcttg aacttccatc tattgtaacg gtaaatggtc gtttcgaaaa atcaactttt
     9361 gtaacaatac cgtagcgaat atctgcacca aatcgttggg cttgtttttt taggtcttcc
     9421 atcattagcg gacccgtaat accctcaggg taccccggaa agttctcaat ctcagttgtg
     9481 gttgtaagct gaccacccgg ctcaaggcct tcatacataa ttggattcag gttagctctt
     9541 gatgcatata tggctgctgt gcaaccggcc ggtcccgatc caattatcag gcacttcgtt
     9601 ttttccatat aagaaattat ttaataatta tttttgtgtt ttcaataatc cagatattat
     9661 cttccttttt tgcctgaatc aaaatatcgt tgctttcgag cctgcactgc ccgtcttttt
     9721 taaaacaggc ctgtgcttca gaataaaaca ataaatccac acggtttccg gaaggtataa
     9781 actgtctttc aaaaattgct gagtcttcgt agtacctaat attcatttcg aaaatatcgt
     9841 cgtataattc cactgatgct gttttagcag taagtgttcc gccggcaatt aggtttgttg
     9901 tttcatcaaa attgataatt agaggtatag ggccgccatc agtaacatct tcagagtata
     9961 tgtaccatcc cttctcaata gtggctttta tatgtaaaac gaagcttgtg tcgttggatt
    10021 ctctgactga aaacgaccag aatacaggtg tggaattctg agctaaaagt atgctcatgt
    10081 tgcccagcaa aataagtatg attaataatt ttttcatctc agcatttcta ttgttgcact
    10141 atcggtaata tgaacttttg caatactagt gccatatcct ccccatatcc ccagagcagg
    10201 gtctccctcg atattcgact ttatcgtagc cgggttggta aaagggttcc cccaagttat
    10261 catgtggcgg tagtatgaat tccagaactc gtagtgttct atatcaattg tggatagacg
    10321 aacagagaca ttggtcccaa ccccgaaagc tagggaattt aaaaactcct cgtcagattg
    10381 ctctgtttcg aaaaacgaaa tctcatccat ccctctggtt ataccaaaat atatcacctg
    10441 agccccgtta tcatataaat catcatcaat gtgggaacca taataaggct tgtaaaattc
    10501 ggtctgctca ccctcaacac gtgtatgata tgcgtaatag ttacctcgtt cgggtgggtc
    10561 ctgatatctg aaacttaaaa tccccagtga atcgtagtcg gtaagtaaag tgaaccaaac
    10621 actgtcgatt gcaaccggct gtggaatacg ggttgttgca aagtagtgat tgtttttcca
    10681 ataaatatcc aagcggtatt ctttcccata ttctccgatg atatcactgc agctatatcc
    10741 aaagtaagga aatttccctg gatagaaact taagctcatt gtgtcagtaa cactgccatc
    10801 gcttactgta acaaccgttg aatctagaac tatcatgtca attacagttt cagcggttat
    10861 cgaatcgaag tagcctgagt tttttgtaag aaacaccacc ggatgttcac cactggcaat
    10921 atacccctcc actaccaatt tctcttcggg gcgtggaagt tccactgaaa tgtcggtttc
    10981 gcaactgaac aatgctagga taactgtcag gataagtgtt atatgtatta ttcttatttt
    11041 cattttaaaa actaaaattc catgttattg caggaagaat tggaataagt gacacttgtt
    11101 ttgcaacagt tcggaatgat tccgtatcaa gattgccttc ataataaaaa taaataaaat
    11161 aggggttctt ccggttgtac acattataaa ccgagaggtt ccattccgat ttccatttat
    11221 cggttttttt atgagtataa gttaccgata catccagtcg gtggtaaggg gcgagccggt
    11281 atgaatttct tggtccatat tcattcacaa tttgcccgtt aattatatat cttgcaattg
    11341 gcaaggtagt agtgtttccg gtagcaaaaa caaatacacc tccaaacgac catttttcgc
    11401 taagttggta gctggcggta accgaaagat catgccgccg atcgtatttg gcagggaaag
    11461 gctctccatt gtcgacaccc tcgaagaagc gcgtagtttt cgaccacgtg taacctaccc
    11521 agccagttac tcgcccggta cgtcgtttaa caaaaagctc aagtccgtat gagttgccat
    11581 ctccgaagat aaaatagttg tctggattat cgttcaagtc atcgccatac attgctccat
    11641 ccatatattc aatcagattc ctgaggtctt tgtagtatat ttctaaggaa gcctcatact
    11701 gattgttata gaagttacgg aagtaaccca ttgcatactg tctgccaaac tgtggcttca
    11761 ccctgtcgct gctgggaacc cacagatcag taggtaaagt cgatgcagaa agcgaagcaa
    11821 gatgaatgaa ctggttgttt tgcatgaacg atgctttgaa actggatgtt tcacctgtcg
    11881 atatttttat ggagaacctt ggctccggtg agtaatagtc gcagactagt tggttagatc
    11941 catacattat tgaatcaacg gtttgcagat tgttctgatc cttaatatac cgggtaaatg
    12001 gtccggtctg aaagaatcct gcaaaacgaa acccagcata cagcgaaatt ttatccgtaa
    12061 ttatataatc atcaccaatg taaacagcag cttcatgggc aaactgttga ttctttcggt
    12121 taaaattgta ctccatatcg ccaaaatcgg catttacagt atttggcgta aaaatgtggt
    12181 aaacataatt cattccgaat tttatcgtgt gtttcgtgtt gggaagccat gtgtaatcct
    12241 gcttcagatt gaaatcgcga atacctgagt tttgtatcag acgaaaccca tctgtaccgt
    12301 cgttattctt catattaata tcggtcgaaa actgataatc gctgaaaacc gtcgttgtgt
    12361 tcaggaaaaa cctgtcgttg aaaagatggt tccaacgcag cgataccatg ccgttgcccc
    12421 agggtatctt cattgtaaat ggcgtttcat tacttttaaa tgtgaatacg tcctttccat
    12481 aatatccact taagaacaga cgatccttgt cggaaaacct gtaattgaat ttcgcattga
    12541 aatcgtagaa ataatacccc gatcctttaa aaggacttgt atcgttaaca aaaggtttta
    12601 ttaaaatgtc gatataggtt cggcgggctg atattaaaaa actgcaagta tctttaatga
    12661 ttggaccttg tgcagtgatt cgtgctgaaa tggttcctat tccaccatcg aaatgatacg
    12721 atttcatatt cccgttatcc atagtaatat cgagaacaga gcttacccgt ccaccgtact
    12781 gagctggcat gccgccctta tatagttcaa catccttgat ggcatcggca ttgaaaaccg
    12841 agaaaaaccc gaagagatgt gctgcattgt aaacaactgc ttcatcgagt aatatcaggt
    12901 tctggtctgg gccaccgcca cgaacataaa atccggaatt tgcatcacct gccgattgta
    12961 caccgggtaa tagctgtata gtttttagaa catccacttc tcccataaac gcagggatgg
    13021 tcttaacctc ttcaactgga agcttattca cgctcatgcg gctgtcctga atgtttcggt
    13081 cggtacgttc tcccgaaaca actacttcat cagtggtata agctgtgctt ttcaagctta
    13141 cattgaaact ctgactgtga tttaagttga tttctttttt ctcgtcggca tatcctataa
    13201 atgagtatgt aactatgtaa ctcccggcag gaagcgaaag agaataaaac ccgtatgtat
    13261 tggttgttgc tccctgtttt aacgattcta caaacacatt tgccccgata agctcttctc
    13321 cactggaagc atccttcaca tatccgctga ttgtgaactt ctgcgaatag cttgctgccg
    13381 cagtaaaaat tataattagt aatagcaaat aatacttcat aggttcctaa aaattcaagc
    13441 tataaatgta ttttaaaaaa gaacatctca ttaaataatt tagattataa ccaaaaaaaa
    13501 tacggtttgt tctaaaaaaa agaatttgca ttgttcaaac acttttataa ctttgcagca
    13561 caatttttcg gggtgtggcg cagttggcta gcgtacttgc atggggtgca aggggtcgga
    13621 agttcaagtc ttctcacccc gacaatgatt ggaaagaaag gaagagtttc atattactgt
    13681 gagactcttc ttgttataat aaggtataaa atatgggtta ctatgtttat gttttagaat
    13741 cgcaatcttc gaagagtttg tatgtagggc aaacagcaaa cccgacctca cggttgcgag
    13801 aacataatag cggtaggagc aagtatacaa ggaacaaagg accatgggaa atgatatttc
    13861 tcaaggattt cccatcaaga attgaagcgg ttaacttcga gaaaaagctg aaagctttga
    13921 aaagccctgg ttgtatcaga aagctaataa taagtggctc agtgggctag ttccgattga
    13981 cctcggaaca ggctcccttg cctgagtgca atgggtcgga agggaacttg tcccgaaaat
    14041 atttcgggaa gtcttctcac cccgacaatg attggaaaga aaggaagagt ttcatattac
    14101 tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacaaagg accatgggaa
    14221 atgatatttc tcaaggattt cccatcaaga attgaagcta aacaacatca aaattctagg
    14281 taaaaatact atcaatccga ttattgcagc aactattgca ctcacaagga ctgctgctgc
    14341 tgctatatct tttattcttt taatatcaat attattctct tttgtaacga aatcagccag
    14401 gttttcgatg acagtattta taatttcact gacgaaaaca aaaccaatcg caaaaataat
    14461 ggcaatccat tcatagtggt taatttttaa tagaattgcg cttaaaatta cagcgcctgt
    14521 aattatcgta tgaattacag catttctttc ttcagtaact acaattctaa gtccgttaat
    14581 tgcatattta aaacttctga gaattttcat agttcatcag tttttgttat tataattaat
    14641 tgatattgtt taaacagcag tatttaatga ttcttctgat acagtttgtg ataataacat
    14701 ataaccggat caggtgttga tggcttgtga agatgcatgc catcacccat gcagtgattc
    14761 cattctttac aattcttgca cataccttgt ttaaagatct tacggtttcg gaaatcccga
    14821 aaacccttgt tccatacctc catgaaatca tcggtataaa tat
//
LOCUS       DAOOQP010000077        14831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_4944_length_14831_cov_5.454792, whole genome shotgun sequence.
ACCESSION   DAOOQP010000077 DAOOQP010000000
VERSION     DAOOQP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_14831_cov_5.454792"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLK75_08685"
     CDS             complement(<1..399)
                     /locus_tag="PLK75_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOY39361.1"
                     /translation="MIVKIVVLALYVIMIIAVGIFGLRKTKSFNDFFLGGGNVGPWMS
                     AFSYGTAYFSAVIFIGFAGKVGWGFGYSGVWIGIINGLLGVGVVWALLGWRIKKMSLD
                     YGVSTMSEFLQKRYDCQFLKMAAVVVIFIFM"
     gene            complement(408..566)
                     /locus_tag="PLK75_08690"
     CDS             complement(408..566)
                     /locus_tag="PLK75_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39362.1"
                     /translation="MKRKLTKNEIVLIVILSLSLIAVAISHKRIGDRLTKVWQIYTQS
                     NTNSETNK"
     gene            complement(744..1745)
                     /locus_tag="PLK75_08695"
     CDS             complement(744..1745)
                     /locus_tag="PLK75_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39363.1"
                     /translation="MKTIGLTILFSACMLTSGILYAQNKTTFTQNNDTIIISTDNGTT
                     VTIVTPNVDQIMEFLESNINNITEQTDSLEKRISVTVLTDEDVIADDTIKKEKRTTII
                     TVVHDSSYNDYEHQCHYSDKPKLFEPYFSVEWGMNNYLNNGVFPDATDAQYSVKPWGS
                     WSCAIGPGIRINAGDNLSLNFSTDVLWYNFKFQDRTTRFEETPSGVVFGNDEGVQYPL
                     RSKLTVTYLNLQFMPMVHFGKDADHFNKKMFRIGAGAYAGYRIDSYAKYVDQDNGVKN
                     KLHYRDNYYLENFRYGVKVLLGVDDFNIFANYDLNNLFSQGKGPQLNAFSIGLNFTI"
     gene            complement(1747..2691)
                     /locus_tag="PLK75_08700"
     CDS             complement(1747..2691)
                     /locus_tag="PLK75_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39364.1"
                     /translation="MSNTIDLNNYEAWLLDFIEGNLSESEENILMAFLKSNPELCDEV
                     DNLTPFTLKADKNEKCSIKKNLKKDFDADTLMISSVEGLLSDEQNAELEQLCQTNEQV
                     IQDYEQYRKTILHPDTTIAYTDKLKLKHHGFAITPLQVLAAAVIVILAASAFALTYFG
                     KTDTIITTALKNRTAEVSNYNIRKAFTDILHTDSNTYVAMVKPVPTIAVPQITDNTMR
                     TALAKLPHSNIEPINVTLAPVQMGYCNETLHAVAGFEKTSTFNLSQWFDKGVISEKLT
                     AFTEKSDIKSPIKLLRKTKEDLLSQDYLAEIRNFKNNK"
     gene            complement(2688..3173)
                     /locus_tag="PLK75_08705"
     CDS             complement(2688..3173)
                     /locus_tag="PLK75_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY39365.1"
                     /translation="MNTKEYNKAVDQYADNLYRFLKAGIGNHQLAEDVLQDSFERLWV
                     NRDKVDNDKVKSFLFTTGYHRLIDVLRKEKRMVPVGEYYDDVVLDSSGYTDTNEILHK
                     ALSRLSPIQRTVVLLRDYEGYSYDEIAAITELNESQVKVYIFRARKFLKEVIGKTENV
                     I"
     gene            complement(3357..7577)
                     /gene="glgP"
                     /locus_tag="PLK75_08710"
     CDS             complement(3357..7577)
                     /gene="glgP"
                     /locus_tag="PLK75_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOY39366.1"
                     /translation="MILPHYLFEISWEVCQKVGGIHTVLATKASLIQSSEHGSLDDRY
                     IFIGPDLWREEKANPEFIEMPELFQEWKQHAIAEGIRIKTGIWKIEQPQIAILVDFTG
                     LFSKKDEIFKSLWEEYKLDSITGQWDYIEPALFGYAAGMVIESFINFYLHIRERAIAH
                     FHEWMTGSGVLYLKQHCPQAGTIFTTHATVLGRCLAGNGRKLYGQLGDFQPEITASEF
                     NVTSKYSLERNAALHADVFTTVSSITANECKYLIGRAPDIVTPNGFNLNIVPPVETIN
                     DTRKFCRDRLLKLAEIMQGPLPAKESVLVGTSGRYEFRNKGIDMFIDSLMLAANSNPD
                     KNIIAYVLVPAYNQGPDKEIVCMLENEAETHHLTSKSICSHILHDYDNDPLVRRLRET
                     GMPDNLFFVFIPAYLNGNDGILNKHYYEVLPGLDLTIFASYYEPWGYTPLESIAFYVP
                     TVTTSLAGFGAWIQSRYPGGNQSCYVVNRDDDNYQEAVNELASHIASLEQLNCEQHDR
                     LRSEAHETSKTALWQNLIVNYFEAFRKTLEIVESRKESFIHIRQTGYIPQFRTDINQP
                     DWQSIEVAASLPEEFNKLRDIMMNIWWSWNTDVVDMFQMMDPELWEKHKYNPIKMLKD
                     ISEMRFAELVADEEFHEVYNRIVKKYETYMSEETYKKAPHIAYFSMEYGFNDNLKIFS
                     GGLGILAGDYLKEASDSEVNMTGIGLLYRNGYFKQKLSVYGEQLSEYTPQRFSNLPIS
                     AARNPDGSWAEVSIVFPGRIVYARIWMVNIGRNKLYLLDTDFEKNQENDREITYQLYG
                     GDNENRLKQEMILGIGGIRLIRLLQLRPDMYHVNEGHAAFIGLERLRVLMHESGLTFS
                     EALDYIRASSLFTTHTPVPAGHDVFDEDLMRTYMFHYPERFNITWDDFMNLGKASPAD
                     KKFSMSYLAANFSQEINGVSLLHGHVSRGIFHNLYHDFLPEELHISYATNGVHYKSWT
                     APKWQEFYINNLGEDIDNWLSDTDRWKILSNVPDSEIWSLRQQQRQILVDYLHKRIRK
                     FWVSRFENPKNMISVLDNLKPQALTIAFARRFATYKRAHLLFKNIERLKEIVNNPKQP
                     VQFIFAGKAHPNDKAGQDLIKYIVEISKQPEFLGKIIFLENYDINLAKRLVQGVDIWL
                     NTPTRPLEASGTSGMKAVLNGVLHFSVLDGWWVEGYKKGAGWALPKERTYQNQDFQDE
                     LDSESIYSMLENEIIPMFYNRNAEGVPEQWVGYIKKSISEVAPQFTTRRMLQDYFSRF
                     YNKLYKRSKQLKADNYQLLSQLNRWKKYITARWKEVELVSLETNRPEAEMAQTGDIIN
                     MHIAMNLNGIKPDDVQIELIITENFFGHRELIGIEEAKLIEEKSGIHYFTISTRLFEP
                     GTINYALRIVPRNDLIPYKADFNLIKWL"
     gene            complement(7596..8786)
                     /locus_tag="PLK75_08715"
     CDS             complement(7596..8786)
                     /locus_tag="PLK75_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOY39367.1"
                     /translation="MKTICFYFQVHQPFRLKRYRFFDIGHDHYYFDDYSNRTIMRKVA
                     EKCYLPANQLFLDLIHKMNGAFKISYSISGVAIEQFEKYAPDVMDSFKRLADTGCVEF
                     IAETYAHSLAALKSEEEFAAQVKIQTGLVEKHLGMTPVSFRNTELIYSDAIGEMVANM
                     GYKVMLTEGAKHVLGWKSPNFMYCNAINPRLKVLLKNFRLSDDIAFRFSNKAWEEWPL
                     TADKYIDWLNSSDDKEEVINLFMDYETFGEHQWAETGIFDFMKAFPEMALHSGNYKFM
                     TPSEIADTLTPMSAIHVPYPISWADEERDLTAWLGNELQDEAFKKLYDLEEKVKATGN
                     EQIIKEWRMLQTSDHFYYLCTKWFSDGDVHKYFNPYDSPYSAFINYMNVISDFIIRLN
                     KLQK"
     gene            complement(8825..10102)
                     /locus_tag="PLK75_08720"
     CDS             complement(8825..10102)
                     /locus_tag="PLK75_08720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY39368.1"
                     /translation="MKVLMFGWEFPPHITGGLGTACYGLTRALSSLGVEMIFVVPRLF
                     GDENAPFMRLRNASDIEIATDTEEQKLFWEKMQYIEVNSRIIPYLDPELYSRLFENTL
                     LNTEDIKYRIFSKKFSFSGEYGPNLMEEVSRYAVIASAIALQNNFEVIHSHDWLTYPA
                     GIAAKEVSGKPLVIHVHATEFDRSGENINQNVYDIERKGMLAADRIITVSNLTRKIVI
                     EKYGISEDKVFTVYNGVQTGNFSEPDFTRGVSEKIVTFLGRITYQKGPDYFVEAANLV
                     INKDPNVRFVMAGSGDMLTRMIKRVAKLGIADKFHFTGFLKGADVHRMFAISDVYVMP
                     SVSEPFGISPLEAMTANVPVIISKQSGVSEILNHAIKIDFWDIDEMADSIYGLLHYEG
                     LTTMFKKYGRSEVEQLKWDHAAFRVKEIYESLV"
     gene            complement(10099..12051)
                     /locus_tag="PLK75_08725"
     CDS             complement(10099..12051)
                     /locus_tag="PLK75_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOY39369.1"
                     /translation="MSYIKFTKDELINLEFSLSRELIRTNRAGSYASTTIIGCNTRKY
                     HGLLVCPMDEIDGERHVLLSSLDESVIQHGAMFNLSIHKYPVIFEPKGHKYAREFFVD
                     PIPMLIYRVGGVVLIKEQLFSVEEERIMTRYTLFEANSPTTLRFKPFLAFRSVHTLTR
                     ENMYTNKKYEKAANGISMKLYESYKPVFMQFSKEVDYVHVPHWYYNIEYIEDQKAGLD
                     FQEDLFVPGYFEVNIERGESIIFSAGLTETDPKILNELFDKESFSRVPRSNYLNCLTN
                     ASQQFVIRNKQKYEITAGFPWFGSWGRDTFISLPGLTLINGHFDTCLKVLQTSSEKLR
                     NGLFPNMESGEEAVYNAADAPLWYFYAVQQYLLFSNNTEAVRQNFYLKMMEIFEAYSK
                     GTSFNIHMCDNGLIYAGADNAALTWMDVKINGIPVTQRRGFTVEINALWYNAVCFLLE
                     FSKSVGNNEFAEKYSYLPAKIKESFTGIFWDEKKKYLADFHDNGYTDWKIRPNMIFAA
                     SLPYQVVDEDIAKSVVDVVKSHLLTPKGLRSLSPKDKDYIGTYEGNQENMDRAYHQGT
                     VWPWLLGSFVDAYLKLHGQSGIVLAEKLFYGFEEEMTRHGIGTVSEVFDGDPPHNPGG
                     AISQAWSVAELLRIHYTIEKMKEEQP"
     gene            12555..13307
                     /locus_tag="PLK75_08730"
     CDS             12555..13307
                     /locus_tag="PLK75_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY39370.1"
                     /translation="MFLSIGLSAQTDHIAVQDVKLENGTTITNYNNGIRKEKVFENTG
                     FETVPILCDSLTDSRDGSTYKIVKIGNQVWMAENLRYLPEVVGRKAGSIDTKDLKYFY
                     VYGFNDTTVSKAKATKNYKTYGVLYNWKAANVSCPKGWHLPSNAEWQQLSDFLGGDNV
                     AGGELKEIGVTLWKKPNIGATDAVGFKARPGGGRHYDKRFYFIGRYGYWWSSTEDGPN
                     TAWARYMWDQASKMHRNNGRKDMGFSVRCVKD"
     gene            13542..>14831
                     /locus_tag="PLK75_08735"
     CDS             13542..>14831
                     /locus_tag="PLK75_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HOY39371.1"
                     /translation="MKKIIISILVFGLVSASAFCQQWGLYTLYSVQNSTAAYLIDTAD
                     SPATYHSWTFSSSKKTGYSSYLTHGDTLVRSYMSGSSGGGGSTGGLQKVLWNGTVYWD
                     ITYANMHHDICPLPNGNVLVIAYDNKTSTQAVAMGANSSRSIKSEKIVELKRTGQNTY
                     QEVWVWYLWDHLCQEYSSSKPNYYSDVALHPELLDINVNTQSEIMHMNGIDYNASKDQ
                     IVFSCHNLNELFVIDHSTTTAQAATHSGGNSGKGGDFLYRWGCPSNYDESGTTNFNVI
                     HDARWIPSDHPTYPNCISAFNNKGGSGSKSASDVIDPPDNGYVYSITSGEPFLPTTYN
                     TRYTSNYSCNNMGGQQRLPNGNTFMCVPASATYLIEVNSAGTSLWNKTVSGTVAQAIR
                     YSKCYVRGPVVTAGASLTSVCSGNAVTLNSSAVSVTET"
BASE COUNT         4108 a         3101 c         3061 g         4561 t
ORIGIN      
        1 cataaaaata aatattacca ctacagctgc catttttaaa aactggcaat cgtatctttt
       61 ctggaggaat tcactcatgg tggacactcc gtaatctaac gacatttttt taatacgcca
      121 tcccagcaaa gcccatacta cgcctactcc cagtaatcca ttaataattc ctatccatac
      181 tcccgagtac ccaaaccccc agccaacttt cccggcaaaa ccaataaaaa tcacggctga
      241 aaaataagct gttccatacg aaaaagccga catccatgga ccaacattcc caccaccaag
      301 gaagaagtcg ttaaacgatt ttgttttgcg taaaccaaaa atacccactg ctattatcat
      361 aataacatag agtgcgagta ctacaatttt aacaatcata atctgagtta tttatttgtt
      421 tcggaatttg tgttgctctg ggtataaatt tgccagactt ttgtaaggcg gtcaccaatt
      481 cttttatgcg aaatagccac tgctatcaac gaaagcgaca gtattacaat aagcacaatc
      541 tcgttttttg tcagttttcg cttcattatt cgcttttaaa aaacaaatgt aaaaaaactt
      601 tatggtttcg gaaactaacc tgttaaaatt ccgaattaca gctcctaagt tagtatttat
      661 ggaggtttca attgcaataa aaaagaaagg aagccgattg gcctcctttg cctgagaaaa
      721 cctgtaaatg cttatgggtt aagctatatg gtgaagttaa gtcctatcga aaatgcattg
      781 agttgtggtc cctttccttg tgagaacaag ttattaaggt cgtagttagc gaaaatattg
      841 aaatcatcca caccaagaag cacttttact ccatagcgga agttttcgag atagtaatta
      901 tcgcggtagt gaagtttgtt ttttacacca ttatcctgat caacgtattt tgcatagctg
      961 tcgatacgat agccggcata ggctcctgcg ccaatacgaa acattttttt gttgaaatgg
     1021 tctgcatctt taccaaaatg aaccattggc ataaactgaa ggttcaaata cgttacagta
     1081 agtttgcttc gcaatgggta ctgcacgcct tcgtcgttgc caaacacaac accggagggc
     1141 gtttcttcga aacgggttgt tctgtcctga aatttaaaat tataccacaa cacatcagta
     1201 ctgaagttca gcgacaaatt atcgccagca ttgatgcgga tacccgggcc aattgcacag
     1261 ctccacgatc cccatggttt tactgagtat tgagcatcgg ttgcatccgg aaaaacacca
     1321 ttgttaagat aattgttcat accccattca actgaaaagt agggttcaaa caatttgggc
     1381 ttatcgctgt aatggcattg atgttcatag tcgttataag aactgtcgtg cacaacggta
     1441 ataatagttg ttctcttttc cttttttatg gtatcgtcgg cgataacatc ttcatcggta
     1501 agaacggtga ccgatattct cttttcaaga ctgtcggttt gttctgtaat gttgttgatg
     1561 ttgctttcca gaaattccat tatctggtca acattgggag ttactattgt aaccgtggtc
     1621 ccattatccg tcgaaataat aattgtgtcg ttgttttgtg taaaggtcgt tttgttttgt
     1681 gcatataaaa tacctgaagt aagcatacat gcactgaata aaattgttaa tcctattgtt
     1741 ttcatgttat ttgttgtttt taaagtttct tatttctgcc agatagtctt gcgaaagcaa
     1801 gtcttctttt gtttttctca ataatttaat tggcgattta atgtcagatt tttcggtaaa
     1861 cgctgtcagt ttttcggaaa ttacaccttt gtcaaaccat tgactcaagt tgaaagtaga
     1921 tgttttttca aatccggcta cggcgtggag cgtttcgttg caatatccca tttgcaccgg
     1981 tgcaagggtc acgttaattg gctctatatt ggagtgcgga agcttagcaa gggctgtccg
     2041 catagtgtta tcggtaattt gaggtacagc gatggtgggt accggcttca ccattgctac
     2101 atatgtgttg ctgtcagtat gcaaaatgtc ggtaaaagct ttgcgaatgt tataattgct
     2161 aacctcggct gtacgatttt taagtgcagt ggtaataatt gtatcggttt tcccaaaata
     2221 cgtcagtgcg aaagcagaag cagcaagaat aacaattaca gcagccgcaa gaacctgaag
     2281 tggtgttata gcaaagccgt gatgcttcag tttgagtttg tcggtgtagg caattgtagt
     2341 atccggatgt aatatggttt ttctgtattg ttcgtaatcc tgaattacct gttcgtttgt
     2401 ctggcaaagt tgttctaact ccgcattttg ttcgtcactt aataggccct caacggaaga
     2461 gatcataagg gtatcggcgt cgaaatcttt tttcaggttc tttttgatgg agcatttttc
     2521 gttcttatca gcctttaagg taaacggggt taagttatcc acttcatcac acaactcggg
     2581 attgcttttt aaaaaagcca taagaatgtt ttcttcgctt tcgctgagat tcccttcgat
     2641 aaaatcgagg agccaggctt cgtagttgtt caagtcaata gtattgctca tatcacattc
     2701 tcggttttac ctattacttc ttttaaaaat ttgcgtgctc tgaaaatata caccttcacc
     2761 tgcgattcat taagctcggt tattgcggcg atttcatcgt aagaatagcc ttcgtaatcg
     2821 cgaagcaaca caaccgttcg ctgtatgggg cttaagcgcg aaagtgcttt atgaagtatt
     2881 tcgttggtgt cggtatatcc ggaactgtcg agcacaacat catcataata ttccccgaca
     2941 ggaaccatgc gtttttcttt tcttaacacg tctataagcc tgtgataccc ggtagtaaac
     3001 aaaaatgatt tcaccttatc gttatccact ttatcgcggt ttacccacaa acgttcaaag
     3061 ctgtcctgta acacatcttc agccaactga tgattgccaa tccctgcttt tagaaagcga
     3121 tacaggttat cggcatattg gtcaacggct ttattgtatt cttttgtgtt catagtcgag
     3181 cataaaacgt acaggttgac gaaaagttac aacaaagtaa aatgtttttt taatagctgc
     3241 tgtaattaag ttctattaac ttgcttaggt tatatggaat cggaataaaa ggagacagaa
     3301 ttcagaatta tatccaagta caattctata gcagtctcaa atcaatttag gacaatctaa
     3361 agccatttaa tcagattaaa atcagcctta taaggtatca agtcgttgcg cggaacaatg
     3421 cgcagtgcat agttaattgt gccgggttca aataatcttg ttgaaatagt gaagtaatga
     3481 atgcccgatt tttcttcaat tagcttggct tcttcaatcc ctataagttc gcggtgtccg
     3541 aaaaagttct cggtaataat gagttcaatt tgtacatcat caggtttaat gccgttgagg
     3601 ttcattgcaa tatgcatatt aatgatatca cctgtctgag ccatttcggc ttcaggacgg
     3661 tttgtctcta aagataccag ctcaacttct ttccagcgtg cagtgatgta ctttttccac
     3721 cggtttagtt gcgatagtaa ctggtaattg tccgctttca gttgtttact gcgtttgtag
     3781 agtttgttat aaaaccgact gaaataatct tgcagcattc ttcgcgttgt gaattgtggt
     3841 gcaacctcag agatagactt tttaatatac ccgacccatt gctcgggaac cccttcagca
     3901 ttacggttat agaacatagg aatgatctcg ttttcgagca tggagtaaat actttcacta
     3961 tcgagctcat cctgaaaatc ctgattctgg tatgttcttt ccttaggtaa agcccaaccg
     4021 gcgccttttt tatagccttc aacccaccaa ccatcgagta cactaaagtg caatacaccg
     4081 ttcaatacgg ctttcatacc gctggttcca ctggcttcaa gaggtcgggt aggagtgttg
     4141 agccagatgt ctacgccctg caccagcctt ttggcaagat taatgtcata attctcgagg
     4201 aaaataattt tacccagaaa ctcgggttgc ttcgaaatct caacaatata ttttatgagg
     4261 tcctgaccgg ctttatcgtt agggtgtgct ttccctgcaa aaatgaactg tacgggttgt
     4321 ttcggattgt ttacgatttc tttcagtctt tcaatatttt taaaaagaag atgtgccctt
     4381 ttataagtgg caaagcgccg tgcgaaggca attgtaagcg cttgtggttt caggttgtcg
     4441 agaactgaaa tcatattttt cgggttttca aaccggctta cccaaaactt tcttatgcgt
     4501 ttatgcaaat agtcaactaa gatttgacgt tgttgctggc gcagagacca aatttcagaa
     4561 tcgggaacat tgcttagaat tttccacctg tcagtatccg ataaccagtt gtcgatgtct
     4621 tcccccaggt tgttaatata gaattcctgc catttgggcg ctgtccacga cttataatga
     4681 acaccattgg tagcatagct aatatgcagc tcctcaggta aaaaatcatg gtacagattg
     4741 tgaaaaatac cacggctgac gtgcccgtgc agcaaactaa ccccgttgat ttcctgagaa
     4801 aagttcgctg ccagatagct catcgagaat tttttatcag cagggcttgc tttcccaaga
     4861 ttcatgaaat catcccaggt tatgttgaac ctttcgggat aatgaaacat ataggtacgc
     4921 ataagatctt cgtcgaaaac gtcatgccct gcgggaacag gcgtatgcgt ggtaaacagc
     4981 gacgatgctc tgatgtagtc gagagcttcg gagaaggtaa gaccgctttc atgcattagt
     5041 acacgaagac gctccagtcc tataaaagcc gcatgccctt cgtttacatg gtacatatcc
     5101 ggtcgtagtt gtaataatcg aatcagcctg atgcctccaa ttccgaggat catttcctgt
     5161 tttagcctgt tttcgttatc accgccatac aattggtagg tgatctccct gtcgttttcc
     5221 tgattttttt caaaatcggt atcaagcagg taaagtttgt ttctgccgat attaaccatc
     5281 caaatacgag catacacgat gcgccctgga aaaacaatgc tcacttccgc ccagcttccg
     5341 tcggggtttc tggctgcgct aatcggcaaa ttgctgaatc gctgcggagt gtattccgaa
     5401 agctgttcac cgtaaacaga gagcttttgt ttaaaatatc cgtttctata aagaagaccg
     5461 atacccgtca tatttacttc actgtcgctg gcttctttaa ggtagtcgcc ggcaagtatt
     5521 cccagaccgc ccgagaatat tttaaggttg tcgttaaaac catattccat gctgaaatac
     5581 gcaatatgag gcgccttttt gtaggtttct tcgctcatat atgtttcgta tttcttcacg
     5641 attctgttgt acacttcatg aaattcttca tcagcgacaa gctctgcgaa gcgcatttca
     5701 gaaatatctt tcagcatttt aatgggattg tacttgtgct tttcccaaag ttcagggtcc
     5761 atcatttgaa acatatcaac cacatcggta ttccacgacc accaaatatt catcattatg
     5821 tcgcggagtt tattgaattc ctcgggtagt gaagcagcca cttcaatact ttgccagtcg
     5881 ggttggttaa tatcggttct aaactggggt atgtaacctg tttgtcgaat atgtataaac
     5941 gactcctttc ttgattcaac aatttccaga gtttttcgga atgcttcgaa gtaatttaca
     6001 attaggtttt gccataacgc agttttggag gtttcgtgtg cttcggagcg aagccggtca
     6061 tgttgttcgc aattgagctg ttccaatgat gcgatgtgcg acgcaagctc atttactgct
     6121 tcctgatagt tgtcatcgtc tctgttaaca acataacagg attggtttcc tccggggtaa
     6181 cgcgactgta tccatgcacc aaatcctgca agacttgtag ttactgtcgg tacataaaaa
     6241 gctatgcttt caagaggggt gtatccccat ggttcataat acgaagcaaa aatagtcaga
     6301 tcaaggcctg gaagtacttc gtagtaatgt ttgttgagta taccatcgtt accgtttagg
     6361 taggcaggga tgaaaacaaa aaacaggtta tcaggcatgc ctgtttcacg aagacgtctt
     6421 actagcgggt cgttatcgta atcatgcaaa atatgactgc atatgctttt tgaggtcaga
     6481 tgatgtgttt ctgcttcgtt ttcgagcatg caaacaattt ctttatcggg accctggtta
     6541 tatgcaggta cgagtacata tgcgataata tttttgtcgg gattcgaatt ggcagcaagc
     6601 atcagcgaat cgataaacat gtcgatacct ttattcctga attcgtaacg tccactggta
     6661 cccacgagaa ccgattcttt tgctggcagc gggccttgca tgatttctgc aagtttcagt
     6721 aatctgtctc tacagaattt ccttgtatca tttattgttt caactggggg tactatgttc
     6781 agattaaatc catttggggt aacaatatcg ggggctcgtc cgattaggta tttacactca
     6841 ttggcagtga tgctgcttac tgtggtgaac acatctgcgt gcaaagctgc atttcgttcc
     6901 agagagtatt ttgaggtgac attgaactcg ctcgccgtta tttccggctg gaagtctccc
     6961 agttgaccgt atagcttgcg tccgtttcct gccaaacatc tgccaagcac agttgcatgg
     7021 gttgtgaaaa tagtccctgc ctgtgggcaa tgctgtttaa gataaagaac accggagccg
     7081 gtcatccatt catgaaaatg tgctatggca cgttcacgta tatgcagata aaagttaata
     7141 aagctttcaa ttaccattcc tgcagcatac ccaaataagg caggttctat gtaatcccat
     7201 tgcccggtta tcgaatcaag cttatactct tcccataaag atttaaaaat ttcatctttt
     7261 ttcgagaata acccggtgaa atcaaccagt atggctattt gaggctgttc aattttccat
     7321 atgccagtct taattctaat accttcggct atggcgtgtt gtttccattc ctgaaacagc
     7381 tcaggcattt cgatgaattc ggggttggct ttttcttctc tccataaatc gggtccgata
     7441 aaaatgtatc tgtcatccag gcttccatgc tccgacgatt gaattaacga tgcttttgtt
     7501 gcaagcacgg tgtgtattcc tcccactttc tgacaaactt cccagcttat ttcgaaaagg
     7561 taatgcggta gaatcataat tgtatttcgg tatttttatt tttgtaattt gttcaggcga
     7621 atgataaaat cactgattac attcatatag tttataaaag cagaatacgg tgagtcgtag
     7681 ggattaaagt atttgtgaac atcaccgtcg gagaaccatt tggtacacag gtaatagaaa
     7741 tggtcgctgg tttgcagcat gcgccattct ttaatgattt gttcatttcc ggtggcttta
     7801 actttttctt caagatcata cagcttttta aaggcttcgt cctgaagttc attaccaagc
     7861 caggcagtga ggtcgcgttc ttcatcggcc catgatatcg gatacggtac gtgtattgcc
     7921 gacatggggg taagtgtgtc ggctatttcg gaaggcgtca tgaatttgta attccctgaa
     7981 tgcaatgcca tttcggggaa agctttcata aaatcaaata tgcccgtttc agcccattga
     8041 tgctccccaa acgtttcata atccataaaa aggttgatta cttcttcctt atcgtcggag
     8101 gagttaagcc agtcgatgta tttatcggct gtaagtggcc actcttccca tgctttattc
     8161 gaaaaacgaa atgcaatatc atcgctgagc cggaagttct tgagcagtac tttcaatctg
     8221 ggattaatag cattgcagta catgaagttt ggacttttcc acccaagaac atgctttgca
     8281 ccttcagtga gcattacttt atatcccatg tttgcaacca tttcaccgat ggcgtcagaa
     8341 tagatcagct cggtgtttct gaaagaaacc ggtgtcatgc ccaagtgctt ttcaaccaga
     8401 cctgtttgaa tttttacctg agcagcaaat tcttcttcgc ttttgagtgc tgcaaggctg
     8461 tgtgcatagg tttcagcaat aaattcaacg cagcctgtat cggcaagacg tttaaaacta
     8521 tccatcacat cgggagcata tttttcgaat tgctcaatgg caacacccga aatggaatag
     8581 cttattttga aagcgccgtt cattttatga attaaatcga gaaacaactg attggcagga
     8641 aggtaacatt tttcggcaac cttgcgcata atagtacggt tcgaataatc gtcgaaataa
     8701 taatggtcgt gcccgatgtc gaaaaagcgg tatctcttca gccgaaatgg ctggtgaacc
     8761 tgaaaataaa agcaaattgt cttcatatct ttctttttta tcctgctgtt acgcaagaaa
     8821 attatcaaac taaactttca taaatctctt taacccggaa agcggcatga tcccatttta
     8881 actgttctac ttcgctacgc ccgtatttct tgaacatggt agtaagtcct tcgtaatgca
     8941 acaatccata tattgagtca gccatttcat cgatatccca gaaatcgatt ttaatggcat
     9001 gattcagaat ttctgatacg cccgactgtt tcgaaataat tacaggaaca ttggcagtca
     9061 tggcctcgag gggtgaaatt ccgaagggtt ctgaaaccga aggcatcaca tacacatcgc
     9121 ttatagcaaa catacggtga acatcggctc ctttcaggaa gcctgtgaaa tgaaatttgt
     9181 cggcaatacc aagtttagcg acgcgtttta tcattcgggt gagcatgtcg ccgctgcctg
     9241 ccatcacaaa acgcacattg gggtctttat tgatgacaag attggctgct tcaacaaaat
     9301 aatcggggcc tttctgataa gtgattcttc ccaggaaagt aactattttt tcgcttacac
     9361 cgcgggtgaa atcgggttcc gaaaaattgc ctgtctgcac tccgttgtac actgtaaata
     9421 ccttgtcttc gctgataccg tatttttcga taacgatttt gcgggtaaga ttgcttacag
     9481 taattattct gtcggcagcg agcatgcctt tacgttcaat atcatacaca ttctggttga
     9541 tattctcacc acttcggtca aattcggtag catgaacatg aattaccagc ggcttacccg
     9601 aaacttcctt ggctgcgatg ccggcagggt atgtgagcca gtcgtgggaa tggataacct
     9661 caaaattatt ctgcagtgct atggcagaag caattactgc gtagcgcgaa acctcttcca
     9721 tcaggttggg tccgtattca cccgaaaaac tgaatttctt actgaaaatg cggtatttta
     9781 tatcttcggt attcagtaat gtgttttcga aaagccttga gtataattca gggtcgagat
     9841 aaggtataat acggctattc acttctatgt actgcatctt ttcccagaat agcttctgct
     9901 cttcggtgtc ggtcgcaatt tcgatatcac tggcgttgcg caagcgcata aaaggtgcat
     9961 tttcgtcgcc aaataatcgt ggtacaacaa aaatcatttc cacacccagc gacgaaagtg
    10021 ctctggtgag cccataacag gcagtaccca ggcctccggt aatatgggga ggaaactccc
    10081 atccgaacat cagtactttc atggctgttc ctccttcatt ttttcgatcg tgtaatgtat
    10141 ccgtaagagt tcggcaacgc tccaagcctg cgaaattgcc ccacccggat tatgcggcgg
    10201 gtctccgtcg aacacttccg atactgtgcc tattccgtga cgggtcattt cttcctcgaa
    10261 tccgtaaaat agcttctctg ccagcacaat accgctttgc ccatgaagtt tgaggtaagc
    10321 atccacaaaa ctacccagaa gccaaggcca aacagtacct tgatggtaag ccctgtccat
    10381 attctcctga ttgccttcgt aagttcctat atagtcttta tcttttggcg acaggctcct
    10441 gagacctttg ggtgtgagaa ggtgcgattt aacaacatcc accactgatt tggcaatatc
    10501 ttcatccact acctgatatg gcaatgaggc tgcaaatatc atgttgggcc ttattttcca
    10561 gtcggtgtat ccgttatcgt gaaaatcggc cagatatttc tttttctcgt cccagaaaat
    10621 accggtaaac gattctttaa tttttgctgg caaataggag tatttttccg caaattcatt
    10681 gttgccgaca ctttttgaaa actccagcag aaagcacacc gcattgtacc acagtgcatt
    10741 gatttcaacg gtgaaacctc ttcgctgtgt taccggaata ccgttaattt ttacatccat
    10801 ccatgtgagt gcagcattat cggctcctgc atatatcaat ccattgtcgc acatatgaat
    10861 gttgaacgaa gtccctttgc tgtacgcttc gaagatctcc atcattttga ggtaaaaatt
    10921 ctgacgaaca gcttcggtat tattgctgaa caacaaatat tgctgcactg cataaaaata
    10981 ccagagcgga gcatcggcag cattgtacac agcctcctcg cccgattcca tgtttgggaa
    11041 tagcccgttg cggagctttt cgctactcgt ttgaagtacc ttcaggcaag tgtcgaaatg
    11101 gccattgatc agtgtaagtc ccggtaatga tatgaaagtg tcgcgacccc aactgccaaa
    11161 ccatgggaag ccagcggtga tttcgtattt ctgtttgttg cggatgacaa attgttgcga
    11221 agcattggta aggcagttca ggtaattgct ccgtggaaca cggctgaacg attctttgtc
    11281 gaacaactca ttcagaattt tcggatcggt ttcggtaagt ccagcactga aaatgatgct
    11341 ttcgccccgt tcaatgttca cttcaaaata cccgggtaca aacaaatctt cctgaaaatc
    11401 gagcccggct ttctggtctt cgatatattc gatgttataa taccagtgcg gaacatgaac
    11461 gtagtcaact tctttcgaaa actgcatgaa cacaggcttg tatgattcat aaagtttcat
    11521 gctgatacca ttggctgctt tttcgtattt tttattggtg tacatatttt cacgagtgag
    11581 agtatgtaca ctccggaatg caagaaaagg cttgaagcgt agagttgttg gactatttgc
    11641 ttcgaacagg gtgtaacgag tcataatacg ctcttcttca accgaaaaaa gctgctcttt
    11701 tatcaaaaca accccaccga cacggtaaat gagcatgggt atagggtcaa caaaaaactc
    11761 ccttgcatat ttatgtcctt tgggttcaaa tataacagga tatttatgga tggagaggtt
    11821 gaacatagcg ccatgctgaa ttaccgactc atcgagcgat gaaagtaata cgtgcctctc
    11881 accatcaatt tcatccatcg gacatacaag caagccatgg tatttacggg tgttgcatcc
    11941 gataatcgtg gtgctggcat acgacccagc cctgttggtc ctgatcagct cccgtgaaag
    12001 agagaattca aggttgatca gttcgtcttt ggtaaactta atatacgaca tacaattaga
    12061 tgacttttca aaagtgtata aaaatacaaa aatatgctgg tttataaaag aatatgttag
    12121 ctaattatta atattgataa tgaaaatgtt tctcttattg aaaacagata gctaaagccg
    12181 ataaaataaa tagccaactt cgcataaaac agggttattg ctcattgaaa cccactgtaa
    12241 aatgaaacga attcatctct catttctatt ttttgcaggc ctcactttaa gtaagaaccc
    12301 ttcactcggt gccgtgtgga agagcatcta aacttcattt tccatttgtt gtttttctat
    12361 tcacgacttc actggcaagg ctcaggtgtg cccatcggga agtgaaaata aaacgataaa
    12421 acagtgtatt tgttataaaa aatatttttt acttttgttg agtttacact aagcaaccaa
    12481 aattattttg tccatgccaa tttttttctc ttaaaaacaa gaaaatggaa aaagaatttt
    12541 attcaattat ttttgtgttt ttatcaattg ggttaagcgc tcaaaccgat cacattgcag
    12601 ttcaagacgt aaaacttgaa aacggtacga ccatcaccaa ttacaacaat ggtatccgca
    12661 aagaaaaagt atttgaaaat accggatttg aaacggtgcc tatactttgc gatagtttga
    12721 ccgactcgcg ggatggaagc acatataaaa tagtaaaaat cggcaatcaa gtgtggatgg
    12781 ctgagaactt acgctattta ccagaagtgg tagggcgtaa agctggatca atagatacaa
    12841 aagatcttaa atacttttat gtttacggtt tcaatgatac cactgtgagt aaagccaaag
    12901 caacaaaaaa ttataaaacc tatggtgtgc tttacaattg gaaagctgca aatgtcagtt
    12961 gtccgaaagg atggcatttg ccaagcaatg ctgaatggca gcagctaagc gattttttgg
    13021 gtggtgataa tgtcgctggt ggtgaattaa aagaaatagg tgttacccta tggaaaaaac
    13081 ctaatattgg cgctaccgat gctgttggct tcaaggctcg tccgggtggt ggtcgccact
    13141 acgataagcg tttctacttc attgggcgct atggctactg gtggagttct accgaggacg
    13201 gtccgaatac cgcttgggcc cgctacatgt gggatcaggc cagtaaaatg caccgaaaca
    13261 acggtcgcaa agatatgggt ttttcggttc gatgtgtcaa ggattagact agctgacaaa
    13321 gtgattcagt tccagcaaag tcaatttttt cttcccaata cccaccgtta tttcgaattt
    13381 agataagcga tagtgctatg taaaattttc aggaggggga attatttatt ctatcggtta
    13441 aaaatcaacg tctgtctatt ttattttacc agaatgttgc cattctgtag ctttgttaac
    13501 tgaaaattta ttcaatgtta attaattctc attttgggtt tatgaaaaaa ataatcattt
    13561 caattttagt attcggtttg gtttcggctt cggcattttg tcagcaatgg ggtttgtaca
    13621 ccttatacag cgttcagaat agcaccgctg cttatcttat cgacactgcc gatagcccgg
    13681 ccacctatca ctcatggacg ttttcgtcat cgaaaaaaac gggttattca agttatctta
    13741 cccacggaga tactttagta cgttcatata tgtcgggttc cagtggtggc ggtggatcca
    13801 cgggaggatt gcagaaggtt ttgtggaatg gaaccgtgta ttgggatatt acctatgcga
    13861 atatgcacca cgatatatgc ccgctcccaa acggaaatgt acttgtgatt gcttacgata
    13921 ataaaacatc tactcaggca gtggcaatgg gagcgaattc gagcagatcg ataaaatcgg
    13981 agaaaattgt tgaacttaaa cgtaccggtc agaatacata tcaggaagtg tgggtgtggt
    14041 acctatggga tcacttgtgt caggaataca gctcctccaa acccaattat tacagcgatg
    14101 ttgccttgca ccctgaattg cttgatataa atgttaatac ccaatcggaa ataatgcaca
    14161 tgaatggaat tgactacaat gcgtcaaaag accagattgt tttcagttgt cataacttaa
    14221 atgagttgtt tgtgattgac cacagtacta caacagcaca ggcagctact catagcggag
    14281 gtaactccgg taaaggcgga gattttcttt accgctgggg ttgcccatcg aattacgatg
    14341 aatcaggcac cacaaatttt aatgtaattc atgacgcacg ttggattcca tccgatcacc
    14401 cgacgtaccc caattgcatt tcggcattca ataataaagg gggaagcggc tcaaaatcag
    14461 cttctgatgt tattgatcct cctgataacg gttacgttta tagtatcact tccggagaac
    14521 cgtttttacc cacaacctac aatacacgct acacaagcaa ttactcatgc aacaatatgg
    14581 gggggcagca acgccttccg aatgggaata catttatgtg tgtacccgct tcggctactt
    14641 accttattga agtaaattct gccggcacaa gcctttggaa taaaaccgta agcggaacag
    14701 tggctcaggc aatccggtac agtaaatgtt atgtcagggg tccggtggtt actgctgggg
    14761 catcactcac ttcggtttgt tccggtaatg cggttaccct gaattcctca gccgtttctg
    14821 tcactgaaac a
//
LOCUS       DAOOQP010000078        13946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_5347_length_13946_cov_5.453891, whole genome shotgun sequence.
ACCESSION   DAOOQP010000078 DAOOQP010000000
VERSION     DAOOQP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5347_length_13946_cov_5.453891"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLK75_08740"
     CDS             complement(<1..627)
                     /locus_tag="PLK75_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39372.1"
                     /translation="MKKIILSIFLFFFFQQIFAQTINGEVLYSDDQFEVIVVHGTHSE
                     RGYAVGFLLADRIADIFNSYLLPAFGSYYETGRMLIQTDAHISIDDDYKTEAEAMADA
                     INDAGVMEMEVDYVDILLANSFLDLQNFVGKDLGLDNGCSSFINWGEATQGTSLNGTP
                     VISRHLDWSNQQAIIRNQVLVVHIPSEENLQPWLLIGFAGQMSVLSGLN"
     gene            complement(739..1932)
                     /locus_tag="PLK75_08745"
     CDS             complement(739..1932)
                     /locus_tag="PLK75_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOY39373.1"
                     /translation="MKKISFLITAILLLSLFRVGYAQQVEDESYTFEIKKQIKTTPVR
                     DQYRSGTCWSFSANAFIETELLRMGKPEVDISEMWMVRHAYQDKAEKYVRVHGSLNFG
                     GGGAFHDVFDTWRKYGVVPEEAYPGLNYGEEKHVHGELDGVLKKFVDGVIENGNKKLS
                     TAWKEAFMGILDAYFGVMPESFTYQGKKYTPKEFADNIGFKPDDYVDITSYTHHPFYK
                     AFILEIPDNWAWGSAQNLPLDEMMQVIDNALNNGYPVCWGADVSEKGFSWSKGIAIVP
                     ADTRPDLDGLERDKWEKLSDKEKNEKLYSFSEILPEMEITQELRQAAFDNYQTTDDHG
                     MVFTGIAFDQKGNKYYLVKNSWNTNNIYEGYFYASEAYVRYKTMNIVVHKDAVPKDIR
                     KKLDI"
     gene            complement(1954..2715)
                     /locus_tag="PLK75_08750"
     CDS             complement(1954..2715)
                     /locus_tag="PLK75_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY39374.1"
                     /translation="MKITVLGSGTSQGIPIIGCNCNVCTSKNPKDKRLRSSVLIDTGK
                     VVILVDAGPDFRQQMLRENVKRLDAIIFTHEHRDHIGGLDDIRAFNYINQAPMDVYAE
                     DRVIKALTREYNYAFAEEKYPGVPKLTMHCINENPFEIAGTEITPIRLMHYNLPILGF
                     RIGGFTYVTDANWIAESELQKMSKTEHLIINALRQFKHISHFSLSEAIDVARQCHAKK
                     TYLTHLSDQMGLHDEINATLPENIFLSYDGMKIII"
     gene            2829..3536
                     /locus_tag="PLK75_08755"
     CDS             2829..3536
                     /locus_tag="PLK75_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOY39375.1"
                     /translation="MDQGELILIPTLLGPGAPAQLLPAAVNQAVCNLRYFVVEELRTA
                     RRFLKQVDKSIDIDSITFFILNEHTPLLNISEYLQPVFSGESVGLMSEAGLPALADPG
                     SALVALAHKQNVKVRPLTGPSSVMLALIASGCNGQNFAFNGYLPVNRKERIQKLIALE
                     RRSADLRQCQIFIETPYRNMHLLEDILLHLKPETRFCVASGITHPDEFIKTLTIREWK
                     KINLNLGKKPSVFIIEA"
     gene            3533..4711
                     /locus_tag="PLK75_08760"
     CDS             3533..4711
                     /locus_tag="PLK75_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY39376.1"
                     /translation="MKKIGLRILQTAIVLGLFIGVYMMNGRLLNSDLRPVTSQKFQDS
                     LFVIRSVFPENKFIASPATNTVIVKNNAGKKVAELLFTMPYCENIKGFGGKIPMIIVL
                     DTERKIEQLVLLSNNETPDWIGSLEDAGFMKTWNGLTPNEAAVKQVDAVSGATFSSTA
                     ILESVKRRCSVYCNEVHKSEELNKKSLLINIAVILTVVFALIQFVFQGKLRKVRWLMH
                     LLNIGVMGFLTVNFLSVAFFYNSLLNGMDLIQKMGLFLVLILSVIIPLFSGKAFYCQY
                     VCPYGSLQYFSGLLIKKKFKIPMKVSRILSYTRYVYLFTLLALLVSGVSIVLEDFEPF
                     SAFAYKFASISAFIIAGGFAVLAVFLNKPWCRYFCPTGAVLELMRKPLIAKTKNEDEE
                     "
     gene            4701..5354
                     /locus_tag="PLK75_08765"
     CDS             4701..5354
                     /locus_tag="PLK75_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY39377.1"
                     /translation="MKNRTLTIFLLLLAIIVLATGNLFQYLNLRDTKNNKTEAMKNDS
                     VIGALDIIHQRKSVRHFTGEPVDTALLVEIVKAGMAAPSARNLQPWAFIVVTDRVLLD
                     SLGNALPYAKMLSTAGAAIVVCGDMNKAATNTKTDYWVQDCSAATENILLATEALGLG
                     AVWTAAFPYDERINPVRRVLNLPENLIPLNVIPIGHPTGEDTPKDKWKPENLHWNKF"
     gene            5357..5917
                     /gene="thpR"
                     /locus_tag="PLK75_08770"
     CDS             5357..5917
                     /gene="thpR"
                     /locus_tag="PLK75_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOY39378.1"
                     /translation="MESKRLFLAIEADDDIAAIAEEIKAANSHLAGLRWAKTGDLHVT
                     LCFLGETPEEKIPEVKDKITRLHKHFMPLELKNPVFCKVSGNRGSGMLWLKFDNCNCF
                     ARMVMHSYRSLLNAKPDHTPTPHVTLARFKNDILSIDMLNREYKYSKIFTGIALFESV
                     LTPHGTEYILLEKFGFNNRVLGNNKF"
     gene            6010..6312
                     /locus_tag="PLK75_08775"
     CDS             6010..6312
                     /locus_tag="PLK75_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39379.1"
                     /translation="MEEQTTQIAEQPEQPVVKGKNGLGLAGFILALIGLVLCWVPVLG
                     YILLLTGFILSTIGLIMGIVKKRKLGLAITGLVLSCVGFILSAVVMAGLMQASHMF"
     gene            6509..6925
                     /locus_tag="PLK75_08780"
     CDS             6509..6925
                     /locus_tag="PLK75_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOY39380.1"
                     /translation="MLARRNNMFMPAIFDEIFNNVRQFDNSVFTTPAFNVKETEKEFV
                     IEAAAPGMDKKDFKIDLKERVLTISAEKEVKEENQEGENYFRKEFCYGSFCKSYQLPE
                     NVNEEKIGASYENGILIVRIPKAEEKEKLSKMIKIS"
     gene            complement(6953..8131)
                     /locus_tag="PLK75_08785"
     CDS             complement(6953..8131)
                     /locus_tag="PLK75_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39381.1"
                     /translation="MRYLSPFFILLIVLTGFSCKQRNKTEKNSNLTEPCKTNTEQLQT
                     NPYDTFSYTAYNDIACFIAGNQLHNKESFLSSYFDNKHWKNYATQFSSNWNTYNNSVI
                     SVIQQWSNEHLVYGDTIFYPFSGPDYNYLNAFFPEANYAVLIGLESAGSIPEPDTVTT
                     DSLANYLDAIHNSIYFNLKVSYFRTKSMKNELNSVSLNGTIPMIMLFLNRHGKEILNI
                     HPVEIDGLGNIINTAPDLEFVHDFHKEFDNGVVFTYRDTTDSTIKKLVYFSMDLSNTG
                     LKDGNLKLVENVSKNNTVFLKAASYLCFQNMFTTVKSAILNNASQIITDPSGMPYMNL
                     RDTWDVTLHGEYSGPIALFSGRKQDELRHDIDSLKPARLPFKFGYHPVHWCMIVANRK
                     "
     gene            complement(8128..9441)
                     /locus_tag="PLK75_08790"
     CDS             complement(8128..9441)
                     /locus_tag="PLK75_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOY39382.1"
                     /translation="MNKTTKVAGIGLITAIAYVIANMVGTGVFTSLGFQLAGGVDNVF
                     AVMMLWFFGAIIAFTGAQVYGELGSVMPRSGGEYHYLSQLYHPSLGFMSGFFSAFIGF
                     AAPIALASMALSQYFSNIYSGIHPKIVAAAIIVLVSGVHIISVKSGSRFQIIFTTLKI
                     LLILFFIVAGFFFTKNPQPISLMPTNVSWAQMISSGFAVSLIFVYYSYSGWNASAYFV
                     SELRNPQKNLPRSLLIGTLVVAALYLLLNLVFLYTAPVSELAGQLDVGFVSAYYIFGE
                     SGGKIVSLFISILLISTISSMIFAGPRVIQVMGEDYKIFGFLARKRNNGVPLNAIILQ
                     STISLTLLLTSTFDALLTYLGFVMNLFAFLTIAGVYIHRIKHPEMHRPYKTWAYPILP
                     AIFMIFVIWNVWFMFQIKTAETLFGLLTLGIGLLIYFIFLNKKRA"
     gene            9516..10397
                     /locus_tag="PLK75_08795"
     CDS             9516..10397
                     /locus_tag="PLK75_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY39383.1"
                     /translation="MKNFIPSLAISVTLMLFLSVSCKNTTHKKPDEKKQDYRYENIEP
                     NDRLLSYNFRDSVNYGMKIVFPDNEAKACILMLHGWNLPAEEWCEKTDFCEKALARGY
                     ILIIPDWQKCNYPLSVYPETKREYWRYPDLHWIIDTIINDLQGNHHLLLDGHNNFVAG
                     ISTGGRGAGLLAFYKPDVFTGMASLSGDFDITSMQEEFLYQAWFGPYEKFPLRWKQEC
                     FAYNCSEYKVPSYIAHGRADKVSPVEQSIAMYDSLTKYHPELGHKAHFPDSAGHDYNF
                     WSSETESVLDFFDSLCR"
     gene            10459..10530
                     /locus_tag="PLK75_08800"
     tRNA            10459..10530
                     /locus_tag="PLK75_08800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10491..10493,aa:Gly,seq:ccc)
     gene            10709..11920
                     /locus_tag="PLK75_08805"
     CDS             10709..11920
                     /locus_tag="PLK75_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOY39384.1"
                     /translation="MVFTKLMLDKRRQRSDSTYRISLRITHKRIPIMIGSDYCVSTFD
                     WDEENCRIKPDCKRFNNVARVNNSLDKFRVNAEQMIDLLTLSGEIDKLSPNELKNRIV
                     NKNRPTTFREFNNKIIKELELSNKLGNASCYIQAQRFLDRYSNQKDLSFEDIDFKFLK
                     SMEAMHLSKNNSLNSLSFYLRTVRAVFNRAIHEGVIRREIYPFDKYSIKETKTKKRAI
                     RKNDINLIRDLNIEENTPMWHARNYFMFSFYCIGMNFADMANLRRSDIIKGRIEYVRA
                     KTGKPYSVKLVEPARDIIDLYDHGQGPKDYVFPIIKREDPALRMKDLQNERKTYNKYL
                     KHIAAACGIEANLTSYVARHSWATIAKDLNQPISVISEGLGHEDSKTTQIYLDSFDDE
                     VIDNANELVTG"
     gene            complement(11973..12986)
                     /locus_tag="PLK75_08810"
     CDS             complement(11973..12986)
                     /locus_tag="PLK75_08810"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOY39385.1"
                     /translation="MINFSHLHEWFKLPDETKVRIFAETSKQIGLPSPPTAEKDWWVV
                     HTLSVIFSLKCANALIFKRGTSLSKAWNLIQRFSEDIDLVLDREFLGFTGELSKNDVK
                     KLRRKSYQFITETFTEELKNKFSELGFKEVTVKYREVENHDQDPLIIEIYYNKLTETD
                     TYIKPGVLVEIGSRSLKEPFTQRTFGTMTSEVFAGKPFSDNPITIPVVNPERTFLEKI
                     FLLHEEFQKDTSKIRYRRLSRHLYDIEKISQTDFATTALQNTDLYYTIVEHRSKFTKI
                     QGVDYANHKPENIKFVPPANLLPLWEKDYQDMRESMLYGETLNFEELIKRLTELQKRI
                     NSI"
     gene            complement(12983..13582)
                     /locus_tag="PLK75_08815"
     CDS             complement(12983..13582)
                     /locus_tag="PLK75_08815"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOY39386.1"
                     /translation="MHISVEKQILARVKRKERGTLLFTESVLKNENPEAVRKALERLV
                     KSGDLHRVATGIYVRPVIDDHIGKVLPNIEEIAIAIAKRDRATIVPTGSYAMYKLGLT
                     NQVPLNVVYYTDTSARKIKIGKQTITFKKASSKNLAFVGEVSMLVIQALRTIGKNQVT
                     NEELKQIKIILNKENPKHLHHDLELAPVWIRQLLKIKDE"
     gene            complement(13650..>13946)
                     /locus_tag="PLK75_08820"
     CDS             complement(13650..>13946)
                     /locus_tag="PLK75_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39387.1"
                     /translation="LEAPIYNLCRRNIFRKKTSISTHTDPLEEDKVLLAAPPLSYDKF
                     QNVRLFRQSTGQIDCICTIINKMKTAEYIVVTIDRLAKYYVFTYADFTTVQKIQ"
BASE COUNT         4153 a         2736 c         2855 g         4202 t
ORIGIN      
        1 attcagtccc gatagcaccg acatttgtcc tgcaaaacca atcagaagcc atggttgaag
       61 gttttcttcg gagggaatat gaaccaccaa cacctggttg cgaattattg cctgctgatt
      121 cgaccagtca agatgtcgcg atatcactgg ggtaccgtta aggctggtgc cttgcgttgc
      181 ttcgccccag ttgatgaagg aagaacaccc gttatcgagt ccaagatctt ttccaacgaa
      241 gttctgcaaa tcaagaaatg aatttgccaa taaaatatcg acgtaatcaa cctccatttc
      301 cataacgcct gcatcattaa tagcgtcggc cattgcctct gcttcggtct tgtaatcatc
      361 gtcaatactt atatgagcat cggtttgtat caacatcctg ccggtttcat aataacttcc
      421 gaaagcaggc agcagataac tgttgaaaat atcagcaatt ctatctgcaa gaaggaatcc
      481 aactgcatac ccgcgctctg agtgggttcc atgaaccaca attacttcga actggtcgtc
      541 ggaataaagc acttcaccat tgatggtttg tgcaaaaatc tgttgaaaaa aaaagaacag
      601 aaaaattgag agaataattt ttttcatagg ttttattaag gtttacacaa aaatacatca
      661 attttaaaca acctgcagag taaattttaa ccgtttaatt aagaaaagca cccgatcagg
      721 agtgcttttc ctaaattact atatgtcgag tttctttctg atatcttttg gtacagcgtc
      781 tttatgcacc acaatgttca ttgttttata gcggacgtaa gcttccgagg catagaaata
      841 gccttcgtaa atattgttgg tgttccagct gttttttaca aggtaatatt tgttcccttt
      901 ctggtcgaag gcaataccgg taaacaccat gccgtggtca tcggttgtct ggtaattgtc
      961 gaatgcagcc tggcgtaact cctgggtaat ttccatttcg ggaagaattt cactgaaact
     1021 gtaaagtttt tcattttttt ctttgtccga tagtttttcc cacttatccc tttccaatcc
     1081 gtccagatca ggtctggtat ctgccggaac aatagcgatt cccttcgacc atgaaaaacc
     1141 tttttcacta acatcggcac cccagcatac agggtatccg ttgttgagtg cattgtcgat
     1201 tacctgcatc atttcgtcga ggggcaggtt ctgagcactt ccccatgccc agttgtcggg
     1261 aatttcgaga atgaaagctt tgtagaaagg atggtgtgtg tatgaggtaa tatccacata
     1321 atcatcgggc ttaaagccaa tattgtctgc gaattcttta ggcgtatatt tcttgccctg
     1381 gtaggtgaac gactccggca ttacgccgaa atatgcatca agaatgccca taaaagcttc
     1441 tttccatgcg gtggaaagtt ttttgttccc attctcaatt acgccatcaa cgaatttctt
     1501 aagtactccg tcgagctcac catgcacatg tttttcttct ccatagttaa ggccagggta
     1561 ggcttcttcg ggaacaactc catattttcg ccatgtgtcg aaaacatcgt ggaaggcacc
     1621 accgccacca aagtttaatg agccgtgcac acgtacatat ttttcggctt tatcctgata
     1681 tgcatggcga accatccaca tctcagatat atccacctcc ggtttaccca ttctgagtaa
     1741 ttctgtttca ataaaagcat tggcactaaa gctccagcag gttcctgaac gatactggtc
     1801 gcgaaccggg gtagttttga tttgcttttt gatttcgaag gtgtaggatt cgtcttcaac
     1861 ttgctgggcg tatccgaccc tgaaaagtga gagcagtaaa attgctgtaa ttagaaaact
     1921 gatttttttc ataacgataa ttgtatttga ttgttaaata ataattttca ttccatcata
     1981 acttaaaaaa atgttttcgg gaagcgttgc gtttatttca tcatgtaacc ccatctgatc
     2041 gctcaggtga gtaagatagg ttttttttgc atgacattgc cgggcaacat caatagcttc
     2101 tgataatgaa aaatgtgaaa tatgcttaaa ttgacgtaat gcattgatta tcagatgttc
     2161 tgtcttcgac attttttgaa gctcgctttc agcaatccaa tttgcgtcag taacataggt
     2221 aaagccacca atgcggaaac ctaaaatggg aagattatag tgcatgagcc tgatgggtgt
     2281 aatttccgtt ccggcaattt cgaatgggtt ttcgttgatg cagtgcattg ttagctttgg
     2341 tacgcccgga tatttttctt cagcaaatgc atagttatat tcccttgtaa gggctttaat
     2401 tactctgtct tcggcataaa cgtccatggg tgcctgatta atgtaattaa aagctcttat
     2461 atcatcaagt ccgccaatat gatcgcggtg ttcgtgtgtg aaaataattg catcgagtcg
     2521 tttaacattt tcgcgaagca tctgctgtcg gaaatcggga cctgcatcaa caagaatcac
     2581 aacctttccc gtgtcgataa gaaccgatga ccggaggcgt ttgtcctttg ggtttttaga
     2641 ggtgcataca ttgcaattgc atccaattat tggaatgccc tgcgatgtgc ccgacccaag
     2701 tacagtgatt ttcaagcctg aaaaatttta ccgaagatag caattcctta taatttccat
     2761 tatgaaaata atcagaatga taatgaatag agttaataaa ttattaaaga ctttgttaat
     2821 tttgtactat ggatcagggt gagcttattt taattccgac attactgggg cccggagcac
     2881 ctgctcagtt gcttcctgct gcggttaatc aggctgtttg caacctcagg tattttgtgg
     2941 tggaggagtt acggacggca cgtagatttt tgaagcaagt tgacaaaagc attgatattg
     3001 attcaattac ttttttcatt ctgaatgaac atactccgtt gcttaacatt agtgagtatc
     3061 tgcaacctgt gttctccgga gaaagtgtgg gacttatgtc ggaggcaggg ttgccggcat
     3121 tggctgatcc ggggtcggct ctggtagccc tggcgcataa acaaaatgta aaagtgcggc
     3181 ctttaacagg gccttcatcg gtaatgctgg cactcattgc ttcgggatgt aatgggcaga
     3241 attttgcttt taacggctat ttaccggtga atcgtaagga aaggattcaa aagcttattg
     3301 cactcgaacg gcgatcggct gatttgcgac agtgccagat attcatcgag acgccgtacc
     3361 gaaatatgca ccttctcgaa gacatacttt tgcacctgaa acccgaaacc agattttgtg
     3421 tagcttcggg cataacgcat cctgatgaat tcataaaaac acttacgatt cgcgaatgga
     3481 aaaagataaa tttgaatctc ggcaaaaaac cttcagtttt tattattgaa gcatgaaaaa
     3541 aataggactt aggatattgc aaacagcaat tgttttgggg ttgttcattg gcgtttatat
     3601 gatgaatggc agattattga atagtgattt acgccccgtg acgtctcaga aatttcaaga
     3661 tagcctgttt gtgataagga gtgtttttcc cgaaaataaa tttatagctt ctcccgcaac
     3721 aaataccgtg attgttaaaa acaacgccgg aaaaaaagtt gctgaactat tgtttaccat
     3781 gccatattgc gaaaacataa agggttttgg agggaaaatc ccaatgataa ttgtgcttga
     3841 taccgaaaga aaaatcgagc aacttgtgct tttgtcaaac aatgaaaccc ccgactggat
     3901 cggctcttta gaagacgcag ggtttatgaa aacctggaat ggactcaccc ccaacgaagc
     3961 tgctgtaaaa caagtggacg ctgtgtcggg tgctaccttt tcgagtactg ccatactcga
     4021 aagcgtaaaa cggaggtgct cggtttattg caacgaagtt cacaaaagtg aagagttaaa
     4081 taaaaagtca cttcttataa acattgctgt gattttgacg gtagtgtttg cattgataca
     4141 gtttgttttt cagggtaagc tccgaaaagt ccgctggctg atgcatctgc ttaatatagg
     4201 tgtcatggga tttttaaccg tgaacttcct tagcgtagca tttttttata actcattgct
     4261 aaatggaatg gatctgattc aaaaaatggg tttatttctg gttctcattt tgtcggtcat
     4321 aattccttta ttctctggca aagcttttta ttgtcagtat gtttgccctt atggttcact
     4381 gcagtatttt tcagggttat tgataaaaaa gaaattcaaa atccctatga aagtttcacg
     4441 gattttaagc tacacaaggt atgtgtatct gtttaccctt cttgcattac ttgtttcagg
     4501 agtaagtata gtgctcgaag attttgagcc attttcggca tttgcatata aatttgcgtc
     4561 aatttcggca tttattattg ccgggggctt tgctgtattg gcagtattcc tgaataaacc
     4621 ctggtgtcgc tatttttgcc ctaccggtgc agtattggaa ttaatgcgaa aacctttaat
     4681 cgctaaaact aaaaacgaag atgaagaata gaactttaac cattttttta ctgttgttgg
     4741 caattattgt gcttgctaca ggtaatttgt tccagtactt gaatttaaga gatacaaaaa
     4801 ataataaaac cgaagctatg aaaaacgatt ccgtcatagg cgccctcgac attattcatc
     4861 agcggaaaag tgtccgtcat tttacaggag aacctgttga tactgctctt ttggttgaga
     4921 ttgttaaagc cggcatggca gctcccagtg cacgaaacct tcaaccatgg gcatttattg
     4981 tagtaaccga cagggtttta ctcgactcac ttgggaatgc tttgccctat gcaaaaatgc
     5041 tttccacagc gggtgccgcc attgtggttt gtggcgatat gaataaagct gctaccaata
     5101 caaaaaccga ttattgggtg caggattgca gtgctgcaac cgaaaacata cttcttgcaa
     5161 ccgaagcact gggtcttggc gctgtgtgga cagcagcatt cccttacgac gaacgtatta
     5221 accctgtgcg cagggtgttg aacctgcccg aaaacctgat cccgcttaat gttatcccca
     5281 ttggccaccc aaccggcgaa gatactccga aagacaaatg gaaaccggaa aacctgcatt
     5341 ggaataaatt ctgattatgg aatcgaaaag acttttttta gccattgaag ccgatgatga
     5401 catagcagca attgctgaag aaataaaagc ggccaacagt caccttgctg ggttacgatg
     5461 ggcgaaaacc ggtgatttac acgttaccct atgctttctc ggagaaacac ccgaagaaaa
     5521 aatccctgag gtgaaagata agattacccg cctgcataaa cattttatgc cattggagct
     5581 taaaaaccct gtattttgta aggtttccgg taacagaggc tccggaatgc tctggctgaa
     5641 atttgacaac tgcaattgct ttgcaagaat ggtaatgcac tcgtatcgaa gcctgctaaa
     5701 tgcgaaacct gatcatactc caacccctca tgttaccctt gccagattta aaaatgacat
     5761 cctttcgatt gatatgttga atcgggaata caaatactct aagattttta caggaatagc
     5821 tttgttcgaa tcagtgttaa ctcctcatgg aactgaatat atattgcttg aaaaatttgg
     5881 ttttaataat cgggtacttg gcaataataa attctgaaaa tttggttaca tggaatttat
     5941 tgtatatttt tagcctaatt aaaattactt ttaattcact caaatactaa cacaaaaact
     6001 taaaaaacta tggaagaaca aactactcaa atcgcagaac aaccagaaca gccggttgta
     6061 aaaggaaaaa acggactggg tctcgcagga tttattcttg ccttgattgg cttggtttta
     6121 tgttgggttc cggtattagg ctatattctt ttattgacag ggttcatttt atcaactatc
     6181 ggacttatca tgggaattgt taaaaagaga aaactgggat tggctattac tggattggtt
     6241 ttatcctgtg ttggctttat tctttctgct gttgttatgg ctggcttaat gcaagcttct
     6301 catatgtttt aacagactta aaatgtttta acaaaagaga ttgatcatgc ggtcaatctc
     6361 tttttttttc tatataggtg acaatatgac gtgtgtatgt ccgtaaaaat gctaaaatgg
     6421 cagtaaaatt gggttggtat atcatttgcc atcctgtaca tgaaattaat taatgttaaa
     6481 taaactaaaa ttataggagg acaaagccat gttagcacgt agaaacaata tgtttatgcc
     6541 ggcaattttc gacgaaattt tcaacaatgt tcgtcagttc gacaacagtg tgttcaccac
     6601 acctgcattc aatgtaaaag aaaccgaaaa agagtttgta attgaagccg cagctccggg
     6661 aatggacaaa aaagatttta agattgacct gaaagagcgc gttcttacca tttcggcaga
     6721 aaaagaagta aaagaagaga atcaggaggg tgaaaactac ttccgtaaag aattttgcta
     6781 cggctcattc tgcaaatcgt atcagcttcc cgaaaatgtg aacgaagaaa aaattggggc
     6841 atcatacgag aacggcattc ttatcgtgag aattcccaaa gcagaggaaa aagaaaaatt
     6901 gtcgaaaatg attaaaatca gctaagcatc agagcccctt cgggggcttt ttttatttcc
     6961 gattggcaac aatcatgcac cagtgcaccg ggtgataccc aaatttaaac ggcaagcgag
     7021 ctggcttaag agagtcgatg tcgtgacgta gttcatcctg ttttctgccg gaaaacaatg
     7081 caatcggacc ggaatactca ccatgcagcg taacatccca tgtatcgcga agattcatgt
     7141 agggcatgcc tgaaggatct gtgataattt gtgatgcatt gtttaaaatg gctgatttaa
     7201 cggttgtgaa catgttttga aaacataagt atgaggcagc ttttaaaaag acagtattgt
     7261 ttttggaaac attttctacc agctttagat ttccgtcttt caatcctgta ttactaagat
     7321 ccatgctgaa gtaaaccagt ttcttaatcg tgctatcagt agtgtcgcgg taagtaaaaa
     7381 ccacaccatt atcaaattcc ttatgaaaat catgaacgaa ttcaagatcg ggggcagtgt
     7441 taattatatt gcccaagcca tcgatttcaa ccggatgaat gttcagaatt tctttcccat
     7501 gcctgttcag aaacaacatg atcattggaa tagtgccatt tagagaaaca ctgttgagtt
     7561 cgttcttcat acttttagtt ctgaaataac tgactttgag gttaaagtaa attgaattat
     7621 gtattgcatc cagataattt gcaaggctat cggtcgtaac agtatccggt tcgggaatac
     7681 tacctgcact ttcgagccct atcagcacgg cataattggc ttcagggaaa aatgcattca
     7741 aatagttata atcaggtccg ctgaaagggt aaaaaatagt atcgccataa accagatgtt
     7801 cattcgacca ttgttgaatc acactaataa ctgaattgtt gtaggtgttc caatttgatg
     7861 aaaattgcgt tgcgtagttc ttccaatgtt tgttatcgaa ataggaagaa agaaatgatt
     7921 ccttattatg aagttgattc cctgcaataa aacaggcaat atcgttatac gcagtgtagg
     7981 agaatgtgtc gtaaggattg gtctgaagct gctctgtatt agttttacat ggttccgtta
     8041 ggttactatt cttttcggtt ttgttgcgct gtttacacga aaatccagtc agaacaatca
     8101 gcaaaataaa gaatggcgat agatatctca tgcgcgtttt ttgttcagaa aaataaaata
     8161 aataagcagt ccgattccca gagtaagcag tccgaagaga gtttcagcgg tttttatctg
     8221 aaacatgaac caaacattcc atataacaaa tatcataaaa atagccggaa gtatcggata
     8281 tgcccatgtt ttgtagggtc ggtgcatttc ggggtgttta atgcggtgaa tatacacacc
     8341 ggcgatggta agaaatgcaa acaaattcat tacaaagccc agataggtga ggagtgcatc
     8401 gaaagtactg gtaagcaaca gggtgaggct aatggtgctt tgaagaatga ttgcattaag
     8461 cggaactcca ttattgcgtt tacgtgctag aaaaccaaat atcttataat cttcgcccat
     8521 tacctgaatt accctcggtc cggcaaaaat cattgagcta atggttgata tcagcaatat
     8581 actgatgaaa agtgagacga tttttccacc actttcacca aagatgtagt aagctgacac
     8641 aaaacctacg tcaagttgtc ctgctaattc cgagactggg gcagtataga ggaataccag
     8701 attaagcagt aaatacaaag ctgcaaccac caatgttcca ataagcagcg acctgggaag
     8761 gtttttctgc gggttgcgca gctcgctaac aaaataggca gaagcattcc atcccgaata
     8821 ggaatagtac acaaaaatca gggacactgc aaaacccgaa ctaatcattt gcgcccacga
     8881 aacattcgtt ggcatcaggc ttatgggctg cggattcttg gtgaagaaaa atccggccac
     8941 tataaaaaag agaatcagta atattttcag ggtggtaaat attatctgaa agcgtgaccc
     9001 cgatttaaca ctgataatgt gaaccccgct taccagaaca ataattgctg cggcaacaat
     9061 cttgggatga attccgctat agatgtttga gaaatattgt gataatgcca tcgaagccag
     9121 tgcaatggga gcagcaaacc cgataaatgc tgagaagaag cccgacatga accccagcga
     9181 aggatggtat agttgcgaca ggtagtgata ttctccgcct gagcgcggca taaccgaacc
     9241 caactcacca tatacctgtg cgcctgtaaa ggcaattatc gccccgaaaa accagagcat
     9301 catcactgca aatacattgt cgactccacc cgctaactga aagcccaggg atgtgaatac
     9361 accggttccc accatgttgg caatcacata ggcaatggct gttatcaggc ctattccggc
     9421 aacttttgta gttttattca tagcacgctt ttaaaagacg caattataag ataaatttgc
     9481 gattgttaca aaaacgccag ttaactcaac agctaatgaa aaattttatc ccaagccttg
     9541 ctatcagtgt taccctgatg cttttcttgt cagtttcctg taaaaacaca acacacaaaa
     9601 aaccggatga gaagaaacaa gactaccggt atgaaaacat agaacccaac gacaggttac
     9661 tttcgtacaa ttttcgcgat tcggtaaact atggaatgaa aatagttttc cccgataatg
     9721 aggcaaaagc ctgtattctg atgctgcatg gatggaacct tccggcggaa gagtggtgcg
     9781 aaaagacgga tttctgcgag aaagcattag cgaggggata catactgatt attcccgact
     9841 ggcaaaagtg caattatcct ttgtcggttt accctgaaac gaaacgcgaa tactggcgtt
     9901 atcctgattt acactggatt atcgacacaa taatcaacga tttacagggc aatcatcatc
     9961 tgctgttgga tggtcataac aactttgtag ccggtatttc gaccggtggg cggggagcag
    10021 gcttgctggc gttttacaaa ccggatgttt ttaccggaat ggcaagcctt tccggtgatt
    10081 tcgacatcac ctcaatgcag gaagaatttt tgtatcaggc atggttcgga ccctacgaaa
    10141 agttcccttt acgatggaaa caagagtgct tcgcctataa ttgcagtgaa tacaaggtac
    10201 cctcgtatat agcccatggc agagctgaca aagtatcacc ggtcgagcaa agtattgcca
    10261 tgtacgactc gctgacgaaa taccacccag aattgggtca taaagcacat tttcctgatt
    10321 ctgccgggca tgactataat ttttggagta gtgaaactga gtctgtactt gatttttttg
    10381 atagtttatg tcgttgaaaa attgcaaatg atttgtcgga tattatcaaa taatattaca
    10441 tttgcacaga aaacttaagc gggagtagct cagtggtaga gcatcagctt cccaagctga
    10501 gggtcgcggg ttcgaccccc gtttcccgct ctcttcgaaa aacgctaaaa tgcttctgat
    10561 tgcttcgatt caggagcatt tatgttttca ttttgttgac aactttttta gtcaaaaatg
    10621 caaaataatg cataaaaaag cattaaaaag ttaaatttgt tagtcaaatt ttattcacac
    10681 gctaacaaat aaatataaaa aataagatat ggtatttaca aaactaatgc tcgacaaacg
    10741 ccgtcagcgc agcgattcta cgtatcgaat cagtctcaga ataacccata aacgaattcc
    10801 aattatgatt ggatcggact attgtgttag cacatttgac tgggatgagg aaaattgccg
    10861 tattaaaccc gattgtaagc gatttaataa cgttgctcgc gtcaacaata gcctggacaa
    10921 atttcgggtt aatgctgaac aaatgattga tttacttaca ttgagtggag agatagataa
    10981 actgtcgcca aacgaattga aaaatcgaat tgtaaataaa aatcgaccta caactttcag
    11041 ggagtttaac aataaaatta tcaaggaact cgaactttcg aataaactgg ggaacgcatc
    11101 ctgctatatt caggcacagc gttttctcga cagatattcg aatcaaaaag atctgagttt
    11161 cgaggatatt gactttaagt ttctgaaaag catggaagcc atgcacctct cgaaaaacaa
    11221 ctctctaaac agcctgagct tctacctgag aacagtgcgg gcggtgttta accgtgcaat
    11281 tcacgaggga gtaatccgcc gcgagattta tccattcgat aaatactcca tcaaggaaac
    11341 caaaaccaag aaacgtgcta ttcgtaaaaa cgacatcaac ctgattcgtg atctcaatat
    11401 tgaggaaaac acacccatgt ggcatgcacg caattacttt atgttctcgt tttattgcat
    11461 cgggatgaac tttgccgaca tggcgaacct ccggcggtct gatattataa agggacgcat
    11521 tgagtatgtg cgtgcgaaaa ccggaaaacc ctattctgta aaattggtgg agccggcacg
    11581 agatattatt gacctgtacg accacggtca gggacccaaa gactatgttt ttcccatcat
    11641 caagcgcgaa gaccccgcat tacgcatgaa agaccttcaa aacgagcgaa aaacctacaa
    11701 taagtacctg aagcatattg cagctgcctg tggtattgaa gctaacctga catcgtacgt
    11761 agcccgacac tcatgggcta ctatagccaa agacctgaac caacccatca gcgtcatctc
    11821 cgaaggcctc ggccacgaag actccaaaac cacccaaatt tacctcgaca gcttcgacga
    11881 tgaagtaatt gacaatgcca atgagttggt tacggggtag ggggtgattt aagtaattac
    11941 agatccatca atttgtgacc cattcgtcat ggttaaatgg agtttattcg tttttgtagc
    12001 tctgtcaatc tttttattag ttcctcaaag ttcaatgttt caccgtaaag catactttct
    12061 ctcatatctt gatagtcttt ttcccagaga ggcaacaagt tggctggtgg aacaaattta
    12121 atgttttcag gtttgtgatt agcataatca actccttgaa tttttgtaaa cttactgcga
    12181 tgttcaacga ttgtgtagta taagtcagtg ttctgtaatg cagttgtggc aaaatcagtt
    12241 tggcttattt tttcaatgtc gtaaagatgg cggcttagtc gtctatatct tattttactc
    12301 gtatcttttt gaaattcttc gtgaagcaga aaaatctttt ccaaaaatgt cctttcagga
    12361 tttacgacag gaatggtaat agggttgtct gaaaatggct tgcctgcaaa aacttctgaa
    12421 gtcatagttc cgaaagtccg ttgtgtaaat ggttctttca aagagcgact tcctatttct
    12481 accaaaacgc ctggttttat gtatgtgtca gtttctgtaa gtttgttgta atatatttca
    12541 ataatcaacg ggtcttggtc gtgattttca acttctcgat attttacggt tacttcttta
    12601 aagcccaatt cagaaaattt gtttttcagt tcttcagtga aagtttcagt gataaactgg
    12661 taggattttc ttctcagttt ttttacgtca ttcttgctca attcgcctgt aaatcctaaa
    12721 aactctctat ccaataccaa atcaatatct tccgaaaagc gttgtatcaa attccaagct
    12781 ttactcaacg atgttcccct tttaaaaatc aaagcatttg cacattttaa agagaaaata
    12841 acagacaaag tatgcactac ccaccaatct ttttcagcgg taggtgggga aggcaagcca
    12901 atttgcttac tggtttctgc gaatattctg acttttgttt cgtctggtaa tttaaaccac
    12961 tcgtgtaaat gcgaaaaatt aatcattcat ctttgatttt taacaattgc ctaatccata
    13021 caggtgccag ctcaaggtcg tgatgcaggt gtttgggatt ttctttattt aaaatgattt
    13081 ttatttgctt cagttcttca tttgttactt ggtttttacc gattgttcgt agtgcctgta
    13141 taaccaacat actaacttcg ccaacaaaag ctaagttttt tgaacttgct tttttaaatg
    13201 taattgtttg tttgccaatt ttaattttcc ttgccgaagt atcggtataa taaaccacgt
    13261 ttagcgggac ttggtttgtt agccccaatt tatacattgc gtaactccct gttggaacaa
    13321 tcgttgctcg gtctcttttt gcaatagcta ttgcaatttc ttcaatattt ggcaatacct
    13381 tcccaatgtg atcatcgata acaggacgaa cgtaaatacc agtggctacc cggtgtaaat
    13441 cacctgattt caccaaacgt tccaaagcct tacgcactgc ttcaggattc tcgtttttta
    13501 aaacactttc tgtaaataat agtgttcctc tttctttgcg tttaactctt gcaagtattt
    13561 gtttctcaac acttatatgc atgtgtaaat catattattt tgtcacaaat ttagtaataa
    13621 tttgtgacag tataaagact atttcgacac tattgaattt tttgtactgt ggtaaaatcg
    13681 gcataggtga acacataata tttagcaagt ctgtcgatgg taactactat gtattcagcg
    13741 gttttcattt tgtttattat ggtgcaaata caatcaattt gtccagttga ttggcgaaat
    13801 aaccggacat tttgaaattt gtcgtaggaa agtgggggag cggcgagaag cactttatct
    13861 tcttcaaggg ggtcagtatg tgtcgaaatg gaagttttct tccgaaatat atttcttctg
    13921 cacaagttgt aaattggtgc ttccaa
//
LOCUS       DAOOQP010000079        13646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_5528_length_13646_cov_6.200132, whole genome shotgun sequence.
ACCESSION   DAOOQP010000079 DAOOQP010000000
VERSION     DAOOQP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_13646_cov_6.200132"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /locus_tag="PLK75_08825"
     CDS             <1..2300
                     /locus_tag="PLK75_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39388.1"
                     /translation="TFGQAFYRIRGYDSQYYQVLINGVGANDPETGRAFWSNWGGLND
                     ATRSQIEYNGLNFSSEGFGDIGGMTNIITRASNYAPSVKVSYSIGNKSYRHRAMFLYS
                     TGLLENGWAFAVSGSHRWAVNGYADGTFYDAWSYFVSAEKIFNKHHSIGFTGFASPNR
                     RGKSSVSTQEVYDLTGDNYYNSYWGYQNGEIRNSRVNDYHQPTMMLTHYWKIDEQKKL
                     TSSVSYMFGKGGATSLNWYDAADPRPDYYRYLPSYFESDDFMFDYLTDKWQNEESFRQ
                     LDWDAFYDANRKNLFTIIDVNGVTGQNLTGNRSKYIIEERRTDVSKLAFNSVFNDQIT
                     ENIRLVGGVNALLYKAEHYKKMFDLLGGDWWLDIDQFAERDFADDYLSQSDLDNLNRP
                     VTVGDKFGYDYVANINNYNIFAQAGFTYKKIDFYGGVTGSYTNFYRTGMMRNGLFPSN
                     SKGDSEHQNFFNYQAKAGATVKITGRHFILVNAGYFTRAPYFENAYISPRTRDNVVEN
                     LCSEKGYTGDLSYAYRSPVVKARLTGYYSEFNDQTEIKSFYHDELRTFVNYVMTNVGK
                     KHYGAEMGVEVKVGATINLQAALGYGRFLYSTRPLVTIAQDNDATVLATNRTVYLKNY
                     HVGGAPELAASVGVKYSDPKYWFVGVNANYFDENYIDINPERRTAEALESFVITDPQW
                     NQILDQEKLDPGFTVDIWGGKSWRIEDYYLGFTLSIDNILNNKNLVTRGFEQLRFDEG
                     DVDKYPTKYMYMYGRTFMLNVYFRF"
     gene            2320..3567
                     /locus_tag="PLK75_08830"
     CDS             2320..3567
                     /locus_tag="PLK75_08830"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOY39389.1"
                     /translation="MKKNVLVFVLFVSLCGLMSCIHNDFDEPPLNVIPEGHVLTIEQI
                     RNMYADSILSGTYLATYKFTGDYSVYAVVTMDDKSGNIYKTAYIQDSTGAINLHLLAS
                     GGLYEGDSVRVYLKGTVLGAYNNMLQIDSVDADKNVIKQATDRDLIPKVVTIPQILSG
                     AYQGQLVQINDVQFIEGDTNQLYSDPVGLTTINRTIEDCNGNTIIVRTSGYASFAGAP
                     VPNGKGNIIAIAGQYREDWQLYIRRTEEVDLSHLRCGEYNALHEEDFSTVENNVVIAI
                     SGWKNIATTGTVQWVGLNNGIDAMAKFVGTGNNTGWLISPAINLDATTGEYLNFISRA
                     FNVAGASLKVYVSTNYTGGTNPEASTWTELSATIANSTTVLQSGDVDLSSYNGNIYIA
                     FKYTATGGATGEFYIDNFVVATE"
     gene            complement(3620..4612)
                     /locus_tag="PLK75_08835"
     CDS             complement(3620..4612)
                     /locus_tag="PLK75_08835"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOY39390.1"
                     /translation="MKIRTYIIVVTIATVIAGCTGNYKNFKHGATGTPGEILLVTSDE
                     YWKTEVGDSLRAVFLAYCPSTPMEEYLFNLVQIPKEKFIELNQRHRNILFTAVSPDFS
                     KAELKVKNDPYAIGQIMVSLSAPSIPELVKLISDNRNLLVDIYKKADSERWLSSFEKY
                     KVQPVIDQLTKLYHVKMVIPKSYSLDVKKDDFAWISYETRKYTMGIFIYTYPFTDTNT
                     FSLDYLLNKRDAFLQKYVPGERKGSYMTTERKYDYPQMAIINQKGNYTAAITGLWRMQ
                     GDFMGGPFVSRTMVDTLRNRVVTVEGFVYYPNEEVRNYVRQLETLLSTMEITAE"
     gene            4779..5123
                     /gene="rbfA"
                     /locus_tag="PLK75_08840"
     CDS             4779..5123
                     /gene="rbfA"
                     /locus_tag="PLK75_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOY39391.1"
                     /translation="MLSKRQNQVGRLIQCELAELLLKEGKNWLPGKLLTVTAVRMSPD
                     LGVAKVYLSIFPSGHATTDLNEIKKQYGHIRHVLGDSIKNQMKRIPELIFYIDDTLDY
                     VQRIDDKLNPKS"
     gene            5120..6352
                     /locus_tag="PLK75_08845"
     CDS             5120..6352
                     /locus_tag="PLK75_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOY39392.1"
                     /translation="MNFSFYIARRYLLSKKSRNAINIISGISVSGVAVGTAALIIILS
                     VFNGFDGLIKNLFTTFDPDIKITSATSKTFVPSPGFIQKLNNDTYIEAYSFCLEENAL
                     LEYDGQQMIATIKGVDSNYSKVTGIDTMVKNGEFFLGDDQFPMAIAGRGVAYKLNLGI
                     DNAFPVKVNIPSRTAELKGNFQDAEGLINKRNIYTSGIFTIQQEYDNKYFIVPITFAR
                     ELLEFDKDITSVEVKLKHPGNAVFVKESLRNFLGATCIVADRNEQHEFLYKVMRTEKW
                     VIFLILCFILIIASFNVIGSLTMLIIDKKKDINILRSLGANDKQIRNVFFYEGMLISF
                     LGAFLGLLIGGTICWLQQQYGIVKLGTEGSFVVDAYPVIMQAGDFIVVFLTVLIIGFL
                     AAWYPVRYITRRYAIAEY"
     gene            6470..7918
                     /gene="sufB"
                     /locus_tag="PLK75_08850"
     CDS             6470..7918
                     /gene="sufB"
                     /locus_tag="PLK75_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOY39393.1"
                     /translation="MEENTDKLLNDVTSGEYKYGFVTDVDHFTIPKGLNENTVRLISE
                     FKQEPEWLLKFRLKAFATWQSMKMPQWANLSIPEIDYQDIIYYAAPKQKETLKSLDEV
                     DPEIKKTFDKLGIPLDEQKRLSGVAIDAVFDSVSVKTTFQETLAEKGIIFCSFSEAVK
                     NHPDLVQKYLGSVVPYSDNYFAALNSAVFSDGSFCYIPLGVRCPMELSTYFRINAANT
                     GQFERTLIVAEEGAYVSYLEGCTAPIRDENQLHAAVVEIIVHKNADVKYSTVQNWYPG
                     DKNGKGGIYNFVTKRGICKGENAHLSWAQVETGSAVTWKYPSTILLGDNSSSEFYSVA
                     VTNNYQQADTGTKMIHLGKNTKSLIVSKGISAGYSQNSYRGLVKVAKNATNARNFSQC
                     DSLLLGDKCGAHTFPYITVDNETAIVEHEATTSKIGEDQIFYCNQRGINTENAIGLIV
                     NGYVREVLNQLPMEFAVEAQKLLQISLEGSVG"
     gene            7954..8460
                     /locus_tag="PLK75_08855"
     CDS             7954..8460
                     /locus_tag="PLK75_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39394.1"
                     /translation="MQNSVFSKELMLEYLNGGISNVDDAITLALSNEQPFAWRSAWIL
                     GHFLNNCDERLIPHRSQLVKAIADASPDGYQRELLRLAAMCGINDSNAGEIADICFAI
                     WEQPRKQSSVRMIAFKILMQISNMYPEMKPELLLITDTYSDTMSPGVKHSISLLRKKI
                     EKEITKQR"
     gene            8472..9221
                     /gene="sufC"
                     /locus_tag="PLK75_08860"
     CDS             8472..9221
                     /gene="sufC"
                     /locus_tag="PLK75_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOY39395.1"
                     /translation="MLSINNLHARINGKEILKGLNLEVKPGEIHAIMGPNGSGKSTLA
                     AVLTGRDLFEVTTGEITYKGKNLFDLSPEDRAREGMFLSFQYPVEIPGVSMVNFMRTA
                     LNEKRKYLGLEPFSSAAFLKYMREMSELVEIDTKLTNRSVNEGFSGGEKKKNEIFQMA
                     ILQPTLSILDETDSGLDIDALRVVANGVNKLKNKDNASIVITHYQRLLDYIVPDKVHI
                     LYNGKIVKTAGKELALELEEKGYEWIKNSVM"
     gene            9240..10580
                     /gene="sufD"
                     /locus_tag="PLK75_08865"
     CDS             9240..10580
                     /gene="sufD"
                     /locus_tag="PLK75_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOY39396.1"
                     /translation="MTLTEKYCDIFSSLLKNSDNGILSRHRMEALGKFREIGIPTHEN
                     ENYKYSDFTKVLEGKYDFIQKQEKFNADLNQYWQCSVPDMDTRVILFSNGWFYKNNDV
                     VTDLPEGLIICSLKEAAEKHAALFEKHYNRYISQNDGFAYMNTMFANDGVFIYVPENM
                     HVKNTIQLVNLTHGFSERFVMQRNLIVMGNNSRMRLMICDHTLNKADNMVNNVTESFL
                     GKQSSLEVYSIQNEPDRSAVIKSLFFHQSTGSKLINLAFSLHGGMLRNNVSVTLAEEN
                     ADTNLYGLFLNDREQHVDNFTSVDHAAPGCTSNQLYKGILDEDAHGAFQGKVLVRKDS
                     QKTVAYQSNKNICLTPSAKMRTKPQLEIYADDVKCSHGATVGQLDDAALFYLRSRGIG
                     TKEARLMLMYAFAEDIVRQIPVLPLKEQISGLVNSRLRGEFSDCNHCLLNCSRH"
     gene            10694..12589
                     /locus_tag="PLK75_08870"
     CDS             10694..12589
                     /locus_tag="PLK75_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39397.1"
                     /translation="MKKTILFLAAFLLVGIFCIQAQVMYTDDFESYTVGEGIAEQEST
                     WWNTWSGTPGSAEDPTVSNTYAYSGTKSIKVFGSNDGVIEFDDLTQGRYRVEFYLYVP
                     AGRQGYYNIMQNFNPSGAGLVWGMQVFIQNGMMTIDGAGEAAATVPYTAGTWIKVQHF
                     IDLDNDWVDMYIDDVLVHAYQWSKGTFNDGSGINKLDAFDFYAYNDGGTCEYYMDNFL
                     IEQVETPVPPSNFTYEIVNDNDVVLTWDAPETGDPENYTIVRDGIQVGTTSDLTFTDE
                     QLYPNDYEYQLYAYYGTNMGYSASAGLLEVTIPGGNERQFVVYEIFTGTWCQYCPTAA
                     QAIDMMEDENLDVAIIEYHGPEGSDEFANPAITARMLYYQPFFEDPDDEDDDFGFPTT
                     IMNGMNGIEGALTTVADMNEYYDYFYEQIKPIPAIYTISPNIEVVSTDPYEYNVTIDV
                     EETFHYYDHPMKLFVVLTESNIAYPWQTLNELNFVCRAMFPDQNGLDLDFSTESTDQH
                     SVDVIVDASYDILNCQLIMFVQDLTTAEIMQAAKIKMSDYVTTPVNQQNSISVYPNPA
                     NDLINLNAAETINSVVISNIAGQPVFASNPSATSYTIPVENLASGIYMISVETNGKSL
                     KKKIVVE"
     gene            12897..>13646
                     /locus_tag="PLK75_08875"
     CDS             12897..>13646
                     /locus_tag="PLK75_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY39398.1"
                     /translation="MGLDLKKIRTDFPILNTTIYGKPLIYFDNAATTQKPLAVIEKIN
                     EMYTRCNSNVHRGVHFLSTCATNEMEAARKTAQKFLNAGSESEIIFTRGTTEAVNLIA
                     FSFSEEFLGEGDEIIVSEMEHHSNLVPWQMACSRKKAKLVQWDMNDNGELSLTRLREL
                     INSKTRIIALTHVSNALGTINPVKEIIEIAHGQGIPVFIDGAQAVQHISVDVQSLDCD
                     FYAFSGHKVYGPTGIGVLYGKSFWLKKLPPYQ"
BASE COUNT         4219 a         2647 c         2875 g         3905 t
ORIGIN      
        1 acactttcgg gcaggcattc tatcgtataa gaggatacga cagccagtat tatcaggtgc
       61 ttattaatgg cgtgggagca aacgacccgg aaacaggaag ggcgttctgg agcaattggg
      121 ggggcttaaa cgatgctacc cgttcgcaga ttgaatacaa tggtcttaat ttttcgagtg
      181 agggatttgg tgatattggc ggcatgacga atattatcac cagggcatcg aattatgctc
      241 caagtgttaa ggtgtcgtat tccatcggta ataaaagtta ccgccaccgc gcaatgtttc
      301 tttactcaac cggattactg gaaaatggct gggcgtttgc cgtatccgga tcccatagat
      361 gggcagtgaa tggctatgcc gacggaactt tttatgatgc ctggtcgtat tttgttagtg
      421 ctgaaaaaat atttaataag catcacagca ttggctttac cgggtttgca tcgccaaaca
      481 gaagaggtaa aagcagtgtt tcaactcagg aagtttatga ccttaccgga gataattatt
      541 ataacagtta ttggggttat caaaatggtg aaatccgtaa ttcgcgagtt aatgattatc
      601 atcagcctac tatgatgctt acgcattact ggaaaattga tgaacagaaa aaactcacaa
      661 gctctgtgtc gtatatgttc gggaaaggtg gtgctacatc gctcaactgg tacgatgctg
      721 ccgatcccag accagattat taccgctatc tcccaagtta tttcgaaagc gacgatttta
      781 tgttcgatta tctcactgac aaatggcaga atgaggagag ttttcgccaa ctcgactggg
      841 atgcattcta tgatgccaac cgaaaaaacc tgtttaccat tattgatgta aatggtgtta
      901 ccggacaaaa ccttacaggg aatcgttcaa aatacatcat cgaagaacgc agaaccgatg
      961 tttcgaaact tgcattcaat tctgttttca acgatcagat taccgaaaac atacgattgg
     1021 ttgggggtgt aaatgcattg ttatataaag cagagcatta caaaaaaatg tttgatctgc
     1081 tgggaggcga ttggtggctc gatatcgacc agtttgctga acgcgatttt gccgacgact
     1141 acttgtcgca aagcgacctc gacaacctca accgccctgt aacagtgggc gataaattcg
     1201 gctatgatta tgttgctaat atcaataatt acaacatttt tgcccaagca ggatttacct
     1261 ataaaaaaat tgatttctac ggaggagtta ccggttcata caccaatttc tacaggaccg
     1321 gaatgatgcg caacggactg ttcccgtcca attcgaaggg agactcggaa catcaaaatt
     1381 ttttcaacta tcaggcaaaa gcaggtgcaa ctgtaaaaat tacaggccga cattttatac
     1441 ttgttaatgc tggttatttt acccgtgctc cttattttga aaatgcctat atcagtcccc
     1501 gtacccgcga caatgttgtc gagaacctgt gttccgaaaa gggatataca ggagacttga
     1561 gctatgctta caggtcgcct gttgtaaagg caaggcttac cggttattat tcagaattca
     1621 acgatcaaac cgaaattaaa agcttctatc atgatgaact tcgaactttt gtaaattatg
     1681 taatgaccaa tgtcggaaaa aagcactatg gtgccgaaat gggggttgaa gtaaaagttg
     1741 gtgcaacaat aaaccttcag gcagcattgg gttacggccg ttttctttac agtacgcggc
     1801 ccctggttac catagcgcag gataacgatg ctactgtgct tgctaccaac cgtaccgtgt
     1861 accttaaaaa ctatcacgtt ggtggggccc cggagcttgc tgcatctgtt ggcgtaaaat
     1921 attccgatcc caaatactgg tttgttggag ttaacgccaa ctattttgat gagaactata
     1981 ttgacatcaa cccggaacgt cgcactgccg aagctcttga aagctttgtt ataaccgatc
     2041 cccagtggaa tcaaatactc gatcaggaaa aacttgaccc cggatttaca gttgatattt
     2101 ggggtggaaa atcatggaga atagaagatt attatctcgg ttttacttta agtatcgaca
     2161 atatactgaa caacaaaaac cttgttacac gaggctttga acaattgcga ttcgacgaag
     2221 gcgatgtgga caaatatccc acaaaataca tgtatatgta cgggcgtact tttatgttga
     2281 atgtatattt cagattttga taattaataa aatgcagata tgaaaaagaa tgttttagtt
     2341 tttgtattat ttgtctcttt atgcgggctt atgtcgtgta ttcacaacga tttcgatgag
     2401 ccaccactga atgtaatccc agaaggtcat gttcttacca ttgagcaaat acggaatatg
     2461 tatgctgaca gtattctttc cggaacgtat ctggcaactt acaaatttac cggcgattat
     2521 tcggtgtatg ccgtggtaac catggatgac aaatcgggta atatttataa aaccgcatat
     2581 attcaggact ccaccggagc tataaatctt catttactgg cttcgggagg tctttatgaa
     2641 ggcgattcgg taagagttta tttaaaaggc actgtgttgg gtgcttataa caatatgctg
     2701 caaatcgact cggtagatgc tgataaaaat gttatcaaac aggctaccga tagagatttg
     2761 ataccaaaag tagttacgat tccgcaaatt ctcagcggag catatcaggg gcaactggtt
     2821 cagataaacg acgtgcaatt tattgaaggc gataccaatc aattgtattc cgatcctgtg
     2881 ggtcttacca cgattaaccg cacaattgaa gattgcaacg ggaatacaat tattgttcgt
     2941 accagtggtt atgcaagttt tgcaggagct cccgttccca atggcaaagg caacattatt
     3001 gccattgcag gacaataccg tgaggactgg caactatata tccgacgtac ggaagaagtt
     3061 gatttatcac acttacgttg cggcgaatac aatgcactgc atgaggagga tttttctacc
     3121 gtggaaaaca acgtggttat tgctatttca ggctggaaaa atattgctac aacgggtaca
     3181 gttcaatggg ttggtttaaa taacggcatc gatgccatgg ccaaatttgt aggaacaggt
     3241 aacaataccg gatggctgat atctcctgct attaacctcg atgctacaac cggtgaatat
     3301 ctgaatttca tttcacgtgc atttaatgtt gccggagcga gtttgaaagt atatgtttct
     3361 accaactata ccggtggtac aaacccggaa gcctcaactt ggacagagtt aagtgcaact
     3421 attgcaaatt caaccactgt gttacagtcg ggagatgttg atctttcatc gtataacggt
     3481 aatatctaca tcgcattcaa atatacagct accggtggag ccacgggaga gttctatatc
     3541 gataactttg tggttgctac tgagtaaaat agtcttgaaa taaaaaaagg ggattgcatt
     3601 atcccctttt tttattctat tattctgcag tgatttccat ggttgacaac aaagtttcga
     3661 gttgccgtac ataattccgg acctcttcgt taggataata cacaaaaccc tcaaccgtaa
     3721 ctactctgtt gcgcaaggtg tcgaccatgg ttctgctaac aaatggccca cccataaaat
     3781 cgccttgcat ccgccataat cctgtaatag cagcggtgta attccctttc tgattaatta
     3841 ttgccatttg gggataatca tatttacgtt ctgtagtcat ataagaacct tttctttctc
     3901 cgggtacata tttctgcaaa aaggcatcgc gtttgttgag aagatagtcc aacgagaaag
     3961 tgttggtatc ggtaaaggga taggtatata taaaaattcc catagtgtat tttcttgttt
     4021 catacgaaat ccaggcaaaa tcatctttct ttacatcaag cgaatacgat ttgggaataa
     4081 ccatttttac atgataaagc tttgtaagct gatcgataac cggctgaacc ttatattttt
     4141 cgaacgaact tagccatctt tcactatcag cttttttata aatatctacc agcaaattcc
     4201 tgttatcact gataagtttt actaactccg gaatacttgg agccgagaga ctaaccatta
     4261 tctgaccaat ggcataagga tcatttttca ccttgagttc tgcttttgaa aaatcaggtg
     4321 atactgcagt aaataatata ttgcggtggc gctggtttaa ttcaataaac ttctcctttg
     4381 gaatttgaac taggttgaac aaatactcct ccatgggagt ggacgggcaa tacgcaagaa
     4441 acacagcacg caacgaatcg cctacttcgg ttttccagta ttcatcactg gttactaaaa
     4501 gtatctctcc gggagtgccc gtagctccat gcttaaagtt tttgtaattg cccgtgcagc
     4561 ctgcaataac tgtagcaatg gttactacta taatataagt tcttattttc atatcgaatt
     4621 tgttaattct ttcaaataat tactgaggta aaggtagtaa atctttctct aatttttaat
     4681 tgcatggtaa aagcaaaata tgcagaaatc aatgtttaga ttacaattat aaacggcatt
     4741 tctgaaactt tagttacatt tggaaaaaaa ttcaacaaat gctttcaaaa cgtcaaaatc
     4801 aggttggcag gctcattcaa tgcgagcttg ctgaattgtt gctgaaagaa ggaaaaaact
     4861 ggctgccggg aaaattactc actgtaacag cagtacgcat gagtccggat cttggcgttg
     4921 ccaaagtgta tctgagtata ttcccatcgg ggcatgctac taccgatttg aatgaaataa
     4981 agaaacaata cggacatata agacatgtac ttggcgactc aataaaaaac caaatgaaac
     5041 gtattccgga acttattttt tacatcgacg acacactgga ctacgttcaa cgcatcgacg
     5101 ataagttaaa ccccaaaagt tgaatttttc attttacata gcacgacggt acctgttgtc
     5161 gaaaaaatcg cgtaacgcca taaatattat ttcggggata tcggtttcag gcgttgcggt
     5221 aggcactgca gctcttatta ttattctttc ggtatttaac ggattcgacg gcctgataaa
     5281 aaatctattt accacctttg atcctgatat aaaaattaca tccgccacat cgaaaacctt
     5341 tgttccatcg ccgggcttta ttcaaaaact aaacaatgat acgtacattg aagcgtattc
     5401 gttctgtctc gaagaaaatg ctttgcttga atacgacggg caacagatga ttgccactat
     5461 aaaaggtgtt gattctaatt atagtaaagt tacgggtatc gacaccatgg ttaaaaacgg
     5521 ggaattcttc cttggcgacg accagtttcc catggctatt gcaggaagag gagttgccta
     5581 taaattaaac cttggaatcg ataatgcttt tcctgtaaaa gtgaacatcc ccagccgcac
     5641 tgctgaacta aaaggtaatt ttcaggatgc tgaaggcctc atcaataaac gaaacatcta
     5701 tacctccggg atatttacca tacaacaaga atatgataat aaatacttta ttgtccccat
     5761 aacgtttgcg cgtgaattgc tggaatttga taaggatata acttcggttg aagtaaaatt
     5821 gaaacacccc ggaaatgcag tattcgtgaa agaaagcctc agaaactttc tgggtgcgac
     5881 atgtattgtg gctgaccgaa acgaacagca tgaatttttg tataaggtca tgagaacaga
     5941 aaaatgggtt atttttttaa tactgtgttt cattctcata attgcctcgt tcaatgttat
     6001 tgggtcgctg accatgctta ttatcgacaa gaaaaaagat atcaatatcc tgcgaagcct
     6061 tggagctaat gacaaacaaa tcagaaacgt gtttttttac gaaggtatgc tgataagctt
     6121 tttaggagct tttttaggac tgcttatcgg ggggacaatc tgctggttac aacaacagta
     6181 tgggattgtt aaactcggca cagagggttc ttttgtggtt gatgcttatc ctgtaataat
     6241 gcaagcaggc gactttattg tagtattttt aactgtactc attataggct ttcttgcggc
     6301 ctggtaccct gtaagatata ttacccgaag gtatgcaata gccgaatatt agtgctttat
     6361 ccgttctgcg gaaatgaaca atacaagttt gaaactgttt gattaaaaaa cgcagttctt
     6421 caggttttga tttatatttt tgtgaaattt attttctgtt tttatagcta tggaagaaaa
     6481 taccgataaa ttgctgaatg acgtcacttc gggagaatac aaatacggat ttgtaacgga
     6541 tgttgaccat ttcacaatac caaagggtct gaatgaaaat acggttcgtt tgatttcgga
     6601 attcaagcaa gagcccgaat ggctgctcaa attccggttg aaagcttttg ctacatggca
     6661 aagcatgaaa atgccgcaat gggcaaacct gagcatcccc gaaatcgact accaggatat
     6721 tatctattat gcggccccaa aacaaaagga aaccctaaaa agcctcgatg aagttgaccc
     6781 ggaaataaaa aaaaccttcg ataagcttgg cattccgctc gatgaacaga agcgcctttc
     6841 gggggtggca attgatgcag ttttcgattc ggtttcggtg aaaacaacat ttcaggaaac
     6901 tcttgccgaa aaaggaataa tattctgttc tttcagcgaa gctgtgaaaa atcaccctga
     6961 ccttgttcaa aaatacctgg ggagcgtagt tccttatagc gacaattact ttgctgcatt
     7021 aaattccgct gttttcagcg atgggtcttt ctgctatata cccctgggag tgcgctgccc
     7081 tatggagcta agtacttatt tcagaattaa tgcagccaat acaggacagt ttgagcgaac
     7141 tctgatagtt gcagaggaag gtgcgtatgt ttcgtacctt gagggatgca cagctcctat
     7201 ccgtgacgaa aaccaactgc atgccgcagt agttgagata atagtacaca aaaatgcgga
     7261 tgtcaaatat tcgactgtac agaactggta tcccggagat aaaaacggaa aaggcgggat
     7321 atacaatttt gttaccaaga gaggtatctg taagggagaa aatgcccatt taagctgggc
     7381 tcaggtagaa accggttctg ctgttacatg gaaatacccc agcaccattc ttttgggcga
     7441 taattcatcc tcagagtttt atagcgttgc tgtaaccaat aattatcagc aagccgacac
     7501 cggcactaaa atgattcatc ttggcaaaaa caccaaaagc cttattgttt cgaaaggaat
     7561 ctctgcaggt tacagccaga attcgtatcg tggtttggtg aaagtggcta aaaatgccac
     7621 caacgcaaga aatttcagtc agtgtgacag ccttttgctg ggcgacaaat gcggggcgca
     7681 caccttccct tatatcactg tcgacaacga aacggcaatt gttgaacatg aggcaaccac
     7741 ttcaaaaata ggagaagacc agattttcta ttgtaatcaa aggggtatta ataccgaaaa
     7801 tgccattggt cttatcgtga acggttatgt aagggaagtt ttaaaccagc tgcctatgga
     7861 atttgccgta gaagcccaaa aactcctgca aataagcctt gaaggtagcg taggatgatg
     7921 aaccaattgc catatcgaaa ttttaaagaa ctgatgcaaa atagtgtatt tagtaaagaa
     7981 cttatgttgg aatacctaaa tgggggaatc agtaacgtgg atgatgctat tacacttgcc
     8041 ctgagcaatg aacagccatt tgcctggcgt tccgcatgga ttcttggtca ctttctaaat
     8101 aattgcgatg aaagattaat cccccaccgc tcgcaattgg ttaaggcgat tgctgatgcc
     8161 agccccgacg gttaccagag agagttattg cgtctggcag ccatgtgtgg cattaatgat
     8221 tcaaacgcag gtgagattgc cgatatttgc tttgcaattt gggaacagcc ccgtaagcaa
     8281 tcttctgtaa ggatgattgc atttaaaatt ttgatgcaaa tatccaacat gtacccggaa
     8341 atgaaaccgg agttattgct gattaccgat acttactctg atacaatgag tccgggggta
     8401 aaacattcaa tttctctgct tcgaaaaaaa atagaaaaag aaattacaaa acaacgttaa
     8461 aaaacccgaa tatgttaagc attaataact tacatgcccg catcaatggc aaagaaattc
     8521 tgaaaggtct aaaccttgaa gttaaaccag gtgaaataca tgcaatcatg ggcccgaatg
     8581 gatcgggtaa atcaacattg gctgcagtat taacaggcag agatttgttt gaggttacaa
     8641 ccggtgaaat aacttataaa ggtaaaaatt tgttcgacct ttcaccggaa gacagagccc
     8701 gcgagggaat gtttctcagc tttcaatacc ccgtcgaaat acccggagtg agtatggtga
     8761 atttcatgcg taccgcatta aacgaaaagc gtaagtattt aggattggag ccctttagct
     8821 ctgctgcctt tctgaaatac atgcgcgaaa tgagtgaact ggtagaaatt gacacaaagc
     8881 tgacaaacag gtcggtaaac gaaggatttt ccggcggtga aaaaaagaaa aacgaaatct
     8941 tccagatggc tattcttcaa cccacacttt ctatacttga cgaaaccgat tctggcctcg
     9001 atatcgatgc gctgcgggta gtagctaatg gtgtgaataa acttaaaaac aaagataatg
     9061 catccattgt tatcacccat taccaaaggc ttctcgacta cattgttccc gacaaggttc
     9121 acattttgta taacgggaaa attgtgaaaa ctgccggcaa agaacttgca ctcgaactgg
     9181 aggaaaaagg atatgaatgg ataaaaaata gtgttatgta gttaataaaa agtattacta
     9241 tgaccttaac cgaaaaatac tgcgatattt tcagttctct cttgaagaac tccgataatg
     9301 gcattttgtc gagacacaga atggaagccc tcggcaaatt cagagaaatc gggattccaa
     9361 cccatgagaa cgagaattat aagtactccg attttaccaa ggtgcttgag ggtaaatacg
     9421 attttattca aaaacaggag aaatttaatg ccgatttaaa ccaatactgg caatgttccg
     9481 ttccggatat ggatacccgg gtaattcttt tttcgaatgg atggttttat aaaaataacg
     9541 atgtcgttac agatttaccc gaaggattga taatttgcag tctgaaagag gccgctgaaa
     9601 aacatgccgc attattcgaa aaacactata ataggtacat ttcccaaaac gatggattcg
     9661 cgtatatgaa taccatgttt gcaaacgatg gcgtttttat ttacgttccc gaaaatatgc
     9721 atgttaaaaa tacgattcag ctggtaaatc tcactcatgg gttttctgaa cgttttgtta
     9781 tgcaaaggaa ccttattgtg atgggcaata acagccgcat gcgcttaatg atatgtgacc
     9841 ataccctcaa taaagccgac aatatggtga ataatgttac tgaatcattc ctcgggaaac
     9901 agtcaagttt agaagtatat tctattcaga atgaacctga ccgttcggca gtaattaaat
     9961 cactattttt ccaccaaagt accggaagca aactaattaa ccttgcattt tcgcttcatg
    10021 gcggcatgtt gcgcaataat gtttcagtta cacttgcaga agaaaatgcc gacacgaatc
    10081 tgtatggact cttccttaac gacagggagc agcatgtcga taatttcaca tcggtcgatc
    10141 atgcagcacc cggttgcaca agcaatcagc tgtacaaagg aattcttgat gaagatgcac
    10201 atggcgcatt tcaaggcaag gtattggttc gcaaagattc gcaaaaaaca gtggcatatc
    10261 agtcaaataa aaacatttgc cttacgcctt cagcaaaaat gagaaccaag ccccagctcg
    10321 aaatttatgc agatgatgta aagtgttcgc acggagccac tgtgggacaa ctcgatgatg
    10381 ctgccctgtt ctatttgcgc tcgcggggta ttgggaccaa agaagcacgt cttatgttga
    10441 tgtatgcttt tgcggaagac attgtccgac aaattccagt gctcccgttg aaagaacaaa
    10501 tttcggggtt agttaattcc cgtttgcgtg gtgagttttc cgattgtaat cattgtttgt
    10561 taaactgctc aaggcattag tcacagagca aattgcattt ctgtgggttc ctccggcaag
    10621 taattctgat tttttcgtac atcattttgt tttttttcat atcattgtgt aataaaatct
    10681 aaaaacaaga aatatgaaaa aaactatcct atttttagcc gcatttttgt tagtaggcat
    10741 cttctgtatt caagcacaag tgatgtatac cgatgatttt gaatcgtaca cggttggtga
    10801 gggtattgcc gaacaggaat cgacttggtg gaatacatgg agtggaactc cggggtctgc
    10861 cgaagatcca acggtttcta atacctacgc ctatagcgga accaaatcaa ttaaagtatt
    10921 tggcagtaac gatggcgtta tcgaatttga cgaccttact caaggtcgat atcgcgttga
    10981 gttttatcta tacgtaccag caggcagaca gggttattac aatatcatgc agaatttcaa
    11041 cccttcggga gcaggtcttg tatggggaat gcaggttttt atccagaatg gtatgatgac
    11101 aatcgacggt gccggtgaag ccgctgcaac agtgccatat actgcgggta cttggattaa
    11161 agttcagcat tttattgatc ttgacaacga ctgggttgac atgtatatcg acgatgttct
    11221 cgttcacgca tatcagtgga gcaagggtac tttcaacgat ggttcgggta ttaacaaact
    11281 cgacgctttc gatttttatg catacaacga cgggggaacc tgtgaatact acatggacaa
    11341 ttttctaatt gagcaggttg aaacacccgt gccaccaagc aattttacct atgaaatcgt
    11401 taatgataac gatgtggtat taacctggga tgctcccgaa accggcgatc ctgaaaatta
    11461 taccattgtg cgagatggca ttcaagtagg cactaccagt gaccttacat ttaccgatga
    11521 acaactctat cctaacgatt acgaatatca gctctatgcc tactatggta caaatatggg
    11581 ttattcagcc tctgccggac tgctcgaagt taccattccc ggtggaaacg aaaggcaatt
    11641 tgtagtatat gaaattttca caggcacatg gtgccagtac tgccccacgg cagcgcaggc
    11701 aattgatatg atggaagacg aaaaccttga tgtcgctatt attgagtatc atggtcctga
    11761 aggctcagac gaattcgcca accccgccat aactgcaaga atgctgtatt accagccctt
    11821 cttcgaagac cccgacgacg aagatgatga ctttggcttc ccgacaacca ttatgaatgg
    11881 tatgaatgga attgaaggtg cacttactac cgttgccgac atgaatgaat attacgatta
    11941 tttctatgag cagattaaac caattccggc tatatataca atttctccca acattgaggt
    12001 tgtttcaacc gatccatacg aatataatgt tacaattgat gttgaagaaa cattccacta
    12061 ctacgatcat ccaatgaagt tatttgttgt tctcactgaa tcgaatattg cctatccatg
    12121 gcaaacactg aacgagctaa attttgtttg tcgggcaatg ttccccgatc aaaacggtct
    12181 tgatcttgat ttttcgacag aatcaaccga tcagcattcg gttgatgtaa ttgttgatgc
    12241 atcatacgat attttgaact gtcagttgat tatgttcgta caagatttga caacggccga
    12301 aattatgcag gcagcaaaaa ttaagatgag cgattatgtc acgactccgg taaatcagca
    12361 gaattcaata tcggtgtacc cgaatccggc aaatgactta atcaatctga atgcagcaga
    12421 aactattaac tcagtagtga taagcaatat cgcaggccaa ccagtgttcg catcaaaccc
    12481 gtctgcaaca tcatacacta ttccggttga gaaccttgct tccggtattt atatgatttc
    12541 ggttgaaaca aacggaaaat cactgaaaaa gaaaattgta gttgaataat tggttagctt
    12601 aatagtggtc agagaattct ggccactatt tttaactttt atacaacaaa tctgagttgt
    12661 tttatgttta gttaatgaat aattttgttg ttttacttaa actttatagt catgggaaaa
    12721 gaaaaatcac cggtaaaaga aaacaaaaaa tcagctcaaa aaagcctcaa agaaaaacgc
    12781 gcagcaaaaa aggaaaagcg tgattcaaaa agggattaac atcccttttt tactttttat
    12841 ttctttgttt tttgtacggg aatagttaac cgggcataaa ttattatgta tgttctatgg
    12901 gattagattt aaaaaaaata agaaccgatt ttccgatttt aaacacaaca atttacggaa
    12961 agccactgat atattttgat aatgcggcaa ctacgcaaaa accgttggct gtaatcgaaa
    13021 aaataaatga aatgtataca cgatgtaatt caaatgtaca ccgtggggtt cattttttaa
    13081 gcacctgtgc tacaaacgaa atggaagccg ccaggaaaac agctcagaaa tttctgaatg
    13141 ccggcagtga atcagaaata attttcacga ggggtactac cgaggcagta aacctgattg
    13201 ccttcagttt ttcggaagaa tttctcgggg aaggcgatga aattattgtt tcagaaatgg
    13261 aacatcattc caatttggta ccatggcaga tggcttgcag tcgcaaaaag gcaaaactgg
    13321 ttcaatggga tatgaatgat aatggagaat tgtcgctaac ccgattacgg gaactaatca
    13381 attccaaaac ccgcattatt gcactaaccc atgtttccaa cgctctgggg acgattaacc
    13441 ccgtgaaaga aatcattgaa attgctcatg ggcaaggtat ccctgttttt atagacggag
    13501 ctcaggcggt gcaacatatt tcggttgatg ttcagtccct tgattgcgat ttttatgctt
    13561 tttcgggtca taaggtttat ggaccaacgg gcataggagt gttgtatggt aaatcattct
    13621 ggctcaagaa attaccaccc tatcaa
//
LOCUS       DAOOQP010000080        13535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_5589_length_13535_cov_5.356973, whole genome shotgun sequence.
ACCESSION   DAOOQP010000080 DAOOQP010000000
VERSION     DAOOQP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_13535_cov_5.356973"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLK75_08880"
     CDS             <1..1165
                     /locus_tag="PLK75_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39399.1"
                     /translation="IHNGCGSLTVQFTDNSTNNPTEWDWDFGDGSSSEEQNPIHTYLA
                     PGHYTVILTVSNDDGSDDITKNEFIHVWPIVEIDIASTEESAEGAADGTATVTIVQGI
                     HPFTFLWDNGGETATIEGLTAGTYCVTVTDSVGCQANMCVEVSLQAVNPLNALFSFEQ
                     SGVCGSSTLSFHDESTGDITSWNWDFGDGTQSNEQNPVHNYVSPGNFTVLLIISDGEN
                     QSTYDAVVEVFEVPSLSFEVTEESSPGAADGEIVLSITGGAAPYQINWSNGQHTATIT
                     GLQAGDYSVAVVDNNGCYATSAVTVPLGTGIAVDVSELFLLYPNPASGYFNLTADHVI
                     EQVTIMDATGRSCFSSNPGKDAVNIDIQHLPSGLYFVVCSTDQFSQVLKLVVR"
     gene            complement(1218..2738)
                     /gene="purH"
                     /locus_tag="PLK75_08885"
     CDS             complement(1218..2738)
                     /gene="purH"
                     /locus_tag="PLK75_08885"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOY39400.1"
                     /translation="MNRQIKNALISVYHKDGLENIIKKLDAAGVTIFSTGGTHKFIAE
                     LGVKAVAVEDVTGYPSILGGRVKTLHPLIFGGILARRDLPEDIHDQAAYNIPPIDLVV
                     VDLYPFEETLRKGASEDDIIEKIDIGGISLIRAAAKNFNDVLVISHRNQYDRLYSLLE
                     DHKGITDLQTRKQFAREAFSVSSNYDAAIFSWFDSGEFTEFRKSFSPQYKLRYGENPH
                     QKGFFFGKLTDMLEQLQGKEISYNNLLDIDAAVNLINDYDNGNAVAILKHNNACGMSC
                     RISLCEAWKDALAGDPVSAYGGIIVTNAIIDLETATEMNKIFFEIIIAPGYTAEALEI
                     LKQKKNRIILILKNTTTPLHQFRSVLNGVLLQESDKHREKISELSYVTDTRPSEVETE
                     DLLFAFKLVKHTKSNAIVLVKNKQLLASGTGQTSRVDALRQAIEKAKHFGFDLNGAVM
                     ASDAFFPFKDSIEIAYEAGIRSVIQPGGSIRDQETIDFCNENKIAMVFTGIRHFKH"
     gene            2938..3603
                     /locus_tag="PLK75_08890"
     CDS             2938..3603
                     /locus_tag="PLK75_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39401.1"
                     /translation="MKTLYTLILLCFAAVHLIAQDTARIAYSFGFNFKNGIYLNFDQF
                     RNNSPVAIESVVIDFDKNKYNDVYDYIDNSKTLQYYNEFGILSETSTASLWGYCRNGK
                     PWVNWSNGFRQIGFIGSVCHFVATIIVYQDMSASPYYDPYSYNYMGSNQYYTSELHQL
                     IITMNTGEILDFDVENVARAISSDTALYNEFNDLSKRKKRNMMFYYIRKFNENHPLYL
                     PSK"
     gene            3617..4477
                     /locus_tag="PLK75_08895"
     CDS             3617..4477
                     /locus_tag="PLK75_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39402.1"
                     /translation="MKKLLLTSLFPVLYACLLTAQVNIPTGSVQEVNTFFNGKTYIVL
                     ENRLMSDYNDMMKAVTANIWSVTPVEFITLKEFESKRKEKNVSFLMINQVYFDADKSN
                     TFFDFLVLIAGGNYPSVNEMPLLCGVPLCYEGEEEETWVPKIPLATRFIQSHMILSKN
                     HPEMNSKNAVEYYISHHAPLSSKILYIPKEQLEVALQSKSAFSAIYPFPFEICETSKT
                     DELISMADSSALILHIISPVDNDQTDKCIKFIINPGTAEIYYYDMHDISANKPPCLLE
                     SDIKKLAKQQ"
     gene            4474..5751
                     /locus_tag="PLK75_08900"
     CDS             4474..5751
                     /locus_tag="PLK75_08900"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOY39403.1"
                     /translation="MNYQEAIRFLFNSLPMYQRIGQAAYKANLDNTLAFDAYFGHPHN
                     NFRTIHVAGTNGKGSVSHMIAAVLQEAGYRTGLYTSPHLKDFRERIRINGEMISENEV
                     IGFVEKHNAIIDTIQPSFFEMTVAMAFDYFARNQVEIAVIEVGMGGRLDSTNIITPLL
                     SVITNIGYDHTQFLGKTLEEIAGEKAGIIKDGIPVIIGETQPETQRVFTDKAASCHAP
                     IVFADTIYNACRKLNSGKYSVYTVNSDITYHLDLGGLYQGKNLQTTLCALDELRKHCK
                     AITKEHIYKALSSVQNTTGLRGRWQVLSENPMVVCDTGHNAEGISYTMQQIASHSFEK
                     LHIVFGVVNDKDLSKVLQLLPKEATYYFTKASIPRALNENELKLIAESTGLHGEAYTS
                     VSEAYDTALRHAGKKDFIYIGGSTFVVAELELD"
     gene            5820..5894
                     /locus_tag="PLK75_08905"
     tRNA            5820..5894
                     /locus_tag="PLK75_08905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5855..5857,aa:Val,seq:tac)
     assembly_gap    6042..6141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7003..8667
                     /locus_tag="PLK75_08910"
     CDS             7003..8667
                     /locus_tag="PLK75_08910"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOY39404.1"
                     /translation="MNQTNQAWGSRIGLILAMAGNAVGFGNFLRFPVQAIQNGGGAFI
                     IPYLVSFLILGLPLLFIEWSIGRYGGNRGFHSTPLMMQSMDRRRMWKYVGVFGLFCNI
                     GIASYYCYLESWTLAYIFHSAVGTFNGMNQHQVVQLFSDYVSIGKTTLIPFEPVVFFL
                     ICLGINVYILSKGLSGGVEKVAKIGMPLLILFGIILAIRAVTLKAGVDGAVNDSSVGL
                     NFLWTPHFDSIYNPKVWFAAASQIFFTLSLGMGSIHAYASYLKRKDDIALNAMSAGWM
                     NEFVEVVLGGAIIIPIAVGYLGIDRVKELATLGGLGLGFKTLPYLFAQWGHVFGAFCG
                     VLWFGLLFFAGITSSLAMGTPVQGFLEDEFKWSRKKTALAFGLIILVLGLPTILFFNQ
                     GVFDEYDYWTGSFALFVFAALESILFAWFFGIKKGWAEINYGADIKVSRIFRFLIAYV
                     TPVLLIIVFLGAVFTPAGNDWAAAFQGLFHGQGWTFDHGSVIGKLMNSGLKEQLSAAT
                     DQVVIAELKYRILLTNLSRMLLVILFVAISLVVRKAYYKHKREGTL"
     gene            8667..8810
                     /locus_tag="PLK75_08915"
     CDS             8667..8810
                     /locus_tag="PLK75_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39405.1"
                     /translation="MNTSALILMLCSICGVTAVTVYFFIKVLTTKPKQEPDSYTDNDE
                     VPR"
     gene            9122..9613
                     /locus_tag="PLK75_08920"
     CDS             9122..9613
                     /locus_tag="PLK75_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39406.1"
                     /translation="MKAHYLLVFIFIVALIPMVSAQQIIRQTICSVGMNTNSGNFVLK
                     QTIGQPSNTSTFKTESGMLRQGFQQPAHINRAIVHYENPIEMQLYPNPAENSFTLLIV
                     GDDLRFSAVALDITGKTLLNMNMILNQKTVVDCSGWAPGLYFINIYSDSRLIASKKIV
                     KTI"
     gene            9617..10270
                     /locus_tag="PLK75_08925"
     CDS             9617..10270
                     /locus_tag="PLK75_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY39407.1"
                     /translation="MKNTFISILFCLIASYSLAQDNVGLNFAQNFTSFRFIDSQGNFD
                     ENMSSDIRFSYGVNYNKIFNKGIFIRPEIGYKNFGATSVMNENYKMSWNLHYADINAG
                     AGYIFNKFALKPYIGASFYFSYLFKAEQMMGQYYYNIWSSGEIKKTDFGINAFAGVVF
                     AFSKTASVFLEFRNTTGLQQLELNSYEGQNQKLFNRAFSLQFGLQFGIPQNEKRFGV"
     gene            10300..11892
                     /locus_tag="PLK75_08930"
     CDS             10300..11892
                     /locus_tag="PLK75_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39408.1"
                     /translation="MRKFKNIVIAAIACFISSFSYGQIVVDGITYQAVAVDVNGREIA
                     GMDAYGNIVANQAIWLRFSILEQSIAGPVVYQEIVQVNTDINGLFTHVIGLGAITDES
                     PSNSILDIDWGIEKHFLKVEMDDVPYDNYVLAGIQQMMAVPYAFFALNVKGIDATDIE
                     NWNTAYYWGNHADVGYLTSYTETDPIFGSSPAFVISTSDIANWNSAFSWGNHADVGYL
                     TSYTETDPLWTASPSFEITNENINQWNSSLLTLNGITATTQLLSTSTDGFFLEWISEG
                     ATHKLNIPLASNSGVDAGLISNSDYNSFNDKVSGSGVFQQIPFWWDQNQLGGDPGLTF
                     NADDNCLRIGDVEENIAGSRLIIKTLNPVSSANGITNIIESHNEEDAQGFGINNFLNC
                     YNATNNGMQVGIRNMIYKEGSGGGYGIYNSITDFGNGDSYAGYFASYKNSIDGINYGV
                     YSLVEGGTINWAGYFVGNLFAQGNVGIGTNTPTSKLSINGGDINILQIGSGIIMKSPD
                     GQCWRVTIDNGGNFVSTAITCP"
     gene            12038..12970
                     /locus_tag="PLK75_08935"
     CDS             12038..12970
                     /locus_tag="PLK75_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39409.1"
                     /translation="MLLPTEIQCLSNCKLCPRECGANRVLEKTGFCKTDALFRVSAVC
                     AHKGEESVISGSKGICNVFFPHCNLQCVYCQNADISANNIDNLPEAQIFDKLITKICS
                     VLDTTENIVGFVSPSHYVMQMKAIIRGILETGRKPVFVYNTNAYDKPDTLKMLEGIID
                     VYLPDFKYTNSSTASMYSQATDYPKAALAAIKEMYRQKGSTLIVDDKGIVQSGIILRH
                     LVLPGMVNQSIEALKCIAEEISPKIHLSLMSQYYPAHFAKNYPEINRSLFEEEYKDVL
                     EACYSLGFYRGWMQDLESTKIFRPDFSAESPFNE"
     gene            complement(13061..>13535)
                     /locus_tag="PLK75_08940"
     CDS             complement(13061..>13535)
                     /locus_tag="PLK75_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39410.1"
                     /translation="IWQDIPGAVGTSLLANPPYTRSYRVWVTSNGLNCNAIISNIATL
                     EVVDPLTKAPIEDVNMPDESEQTPEPNTEVLASMNLYPNPFRNELNIYLEDCSAGPVV
                     IEVYNLTGSLIYRLDLETETDSFFWQMDISDWAPSIYMFRIENGNQVMVKRAVKN"
BASE COUNT         3990 a         2587 c         2751 g         4107 t
ORIGIN      
        1 cattcacaat ggatgtgggt ctcttaccgt tcagtttacc gataacagta ccaataaccc
       61 gacagaatgg gattgggatt ttggtgatgg cagcagctcg gaagaacaga acccaattca
      121 cacctatctt gcccctggcc attacacagt aattcttact gtttcgaatg acgacggtag
      181 tgatgacatt accaaaaatg aatttattca tgtgtggccg attgttgaaa tagatattgc
      241 ctctacagaa gaaagcgccg aaggtgctgc cgatggcaca gcaactgtta ctatagttca
      301 gggcattcac cctttcactt tcctttggga caacggaggc gaaacggcaa ccatcgaagg
      361 cctgacggca ggtacctatt gtgttaccgt taccgactct gtaggctgtc aagccaacat
      421 gtgtgttgaa gttagccttc aggctgtaaa cccattaaat gcattgtttt catttgaaca
      481 aagcggagtt tgtggttcat caacgcttag cttccacgat gaaagcaccg gtgatattac
      541 ctcatggaac tgggatttcg gcgacggaac acagtcgaat gagcaaaacc ctgttcataa
      601 ttatgtaagc cccggaaact ttacggtatt gcttataatc agcgatggtg aaaatcaaag
      661 tacctacgat gccgttgttg aagtattcga ggtgccgtct ctttcttttg aagttaccga
      721 agaaagcagc cccggtgctg ccgatggaga aatcgtactt agcattaccg gaggagcagc
      781 accgtatcag attaactggt cgaacggaca gcatactgct accattacgg gacttcaggc
      841 tggcgactac tcggttgctg tggtcgataa caacgggtgt tatgccactt cagcggttac
      901 cgttccgctg ggaacaggta ttgcagttga cgtcagcgaa ttgtttttgc tttatcccaa
      961 ccctgcatcc ggatatttta atttgaccgc cgaccatgta attgaacagg tcaccattat
     1021 ggacgcaaca gggcgaagct gttttagcag taatccagga aaagatgctg taaacattga
     1081 catacaacat cttccttcgg gtttatattt tgtggtttgc agtactgacc aattttcgca
     1141 ggtgcttaaa cttgtggtgc gataaacctt tcaatagcaa cacaaaaaat actgcagtag
     1201 aagtattgcg gtatttttta gtgtttaaag tgtcgtattc cggtgaaaac cattgcaatt
     1261 ttattctcat tgcagaagtc gatggtttcc tgatcacgga ttgaaccacc gggttgtatt
     1321 acacttcgta ttccggcttc ataagcaatt tcaatactgt ctttaaatgg aaagaaagcg
     1381 tcggaagcca ttacagcacc attcaggtcg aacccgaaat gctttgcttt ttcaattgcc
     1441 tgacgaagcg catcaacacg tgatgtttga ccggttccac tcgccagcag ttgcttgttt
     1501 tttacaagta caatggcatt cgattttgta tgctttacca atttaaatgc aaacagcaga
     1561 tcttcggtct cgacttccga aggccgggta tcggtaacgt agctcagttc agaaattttt
     1621 tcgcgatgct tatcgctttc ctgaagcaaa acaccgttta acaccgaccg gaactggtgc
     1681 aggggtgttg tggtattttt aagtatgaga ataatccggt tctttttctg cttcagtatt
     1741 tcgagagcct cggcagtata tcccggagca ataattatct cgaaaaatat tttattcatc
     1801 tctgttgcag tttccaaatc gatgatagca ttggtaacta tgatacctcc gtaagccgaa
     1861 acagggtcgc ctgccagagc gtctttccat gcctcgcata ggctgatgcg gcaagacatt
     1921 ccgcaagcat tattatgttt aaggatagca acggcgttcc cattatcata atcgttgata
     1981 aggttaacag cagcatcaat atcgagcaga ttgttgtacg atatttcttt gccctgtaat
     2041 tgctcaagca tatctgtaag ctttccgaag aaaaagcctt tctgatgtgg gttttcaccg
     2101 tagcgaagtt tgtactgcgg actgaagctt ttacggaatt cggtgaactc accgctatcg
     2161 aaccaggaaa atattgcagc atcatagttt gagcttacgc tgaacgcctc acgtgcaaac
     2221 tgcttcctcg tttgcaaatc ggtaattcct ttatggtctt cgagcagact gtacaggcgg
     2281 tcgtattggt tgcggtgcga tattacaagc acatcattga aattttttgc ggctgcacgt
     2341 atcagtgaaa taccaccaat atcgattttt tcgataatat catcctcgct tgcaccttta
     2401 cggagtgtct cttcaaacgg gtataggtcg acaacaacca gatcgattgg ggggatattg
     2461 tacgccgcct gatcatgaat atcttcgggc agatcgcgcc gtgcaagaat acctccaaaa
     2521 ataaggggat gaagtgtttt aacacgacca cccaaaatag aagggtatcc ggtaacatcc
     2581 tccactgcaa cagcttttac accaagttca gcaataaact tatgggtacc tcccgtcgaa
     2641 aaaatggtga cacctgcagc atcaagtttt tttatgatgt tttccagacc atccttatga
     2701 tacactgaga taagcgcgtt tttaatctgt cggttcatac ttcgttattt tgaccgtaaa
     2761 aatagctttt ttgctgaacc ggttttaata catttggcag ccagccaaaa aaaatatccc
     2821 catttattaa caacaccgcg gcatagaaaa tgttttaaat gtgttcgcaa attgttcact
     2881 attggttttt atgactgaaa tgattaaaac ttttatcgta tttttacatc aacaacaatg
     2941 aagacattat atactctgat attgctctgc ttcgcagctg tgcacctgat agcgcaggat
     3001 actgcaagaa ttgcctattc atttgggttc aattttaaaa acggaattta cctgaacttc
     3061 gaccagttca ggaataactc tcccgttgca attgaatcgg ttgttattga ttttgataag
     3121 aataaataca atgacgttta cgactatatt gataattcga aaacgctaca atattacaac
     3181 gaatttggga ttttatcgga aacaagcaca gcttctcttt gggggtattg ccgtaatggt
     3241 aagccatggg taaactggag taacggtttc aggcaaatcg ggttcatagg gtctgtatgt
     3301 catttcgttg ccacaattat tgtgtatcag gatatgagcg catctcccta ttatgatccg
     3361 tattcctata actacatggg ttcaaatcaa tattatacaa gcgagcttca ccaactcatc
     3421 attactatga ataccggaga gatcctggat tttgatgtag aaaatgtagc acgggcaatc
     3481 agcagcgaca cagcacttta caatgaattc aacgatttaa gcaagcgtaa aaaacgtaat
     3541 atgatgtttt attatattag aaaattcaac gaaaatcacc cgttatactt accatcaaaa
     3601 tagatattca attataatga aaaagctact tttaacatcg ctattcccgg ttctttatgc
     3661 atgcttatta acagctcagg taaatattcc caccggtagt gttcaggaag tcaatacgtt
     3721 tttcaatggg aaaacctata tcgtactcga gaacaggctt atgtcggatt acaacgatat
     3781 gatgaaagca gtaacagcaa atatctggtc ggttacaccc gtagaattta tcacattaaa
     3841 agagtttgaa tcgaaacgta aagaaaaaaa cgttagtttt ctgatgataa atcaggtgta
     3901 tttcgatgct gacaaatcga ataccttctt cgactttttg gttttaattg ccggtggcaa
     3961 ttatccctct gtaaatgaaa tgcctttact ttgtggtgtc cctctctgct atgaaggaga
     4021 agaagaggaa acatgggttc ccaaaattcc actggcaacc aggttcattc agtcgcacat
     4081 gattctttca aaaaatcatc ctgaaatgaa ttcgaaaaat gcagttgaat actatatttc
     4141 tcaccacgcc cccctgagca gtaaaatcct ttacattccc aaagaacaac ttgaagtggc
     4201 attacaaagt aagagtgctt tcagtgccat ttaccctttt ccattcgaga tttgcgaaac
     4261 ctcaaaaacc gatgagctta tttcgatggc agatagctcc gctcttattc ttcacataat
     4321 atcgccggta gataatgatc aaaccgacaa atgcataaaa ttcattatca atccgggaac
     4381 agcagaaatt tattattacg atatgcatga tatcagtgcg aataaacccc cttgcctgct
     4441 cgaaagtgat attaaaaaac ttgccaaaca acaatgaatt atcaggaggc tatccggttt
     4501 ttgttcaatt cattgcctat gtatcagcgt ataggtcagg ctgcatataa agccaatctc
     4561 gacaacacct tagcatttga cgcatatttc ggacacccgc acaataactt tcgcaccatc
     4621 catgttgcgg gtaccaatgg gaaaggttcg gtgtcgcaca tgatagcagc agtgttgcaa
     4681 gaggctggct acagaacggg gctgtacacc tctccccacc tgaaagattt cagagaacgt
     4741 attcgcatca acggagaaat gatcagcgaa aatgaggtta ttggttttgt tgaaaagcat
     4801 aatgcaataa ttgacacgat tcagccctcg tttttcgaaa tgactgtagc catggctttc
     4861 gattattttg ctcgaaatca ggttgaaatt gcagtgatcg aagttggcat gggaggtcgc
     4921 ctcgattcaa ccaatattat tactccgctt ttatcggtta tcacgaacat aggttacgat
     4981 catacccagt ttctcgggaa aacccttgaa gaaatagccg gtgaaaaagc gggtattatt
     5041 aaagatggta ttccagttat aataggtgaa acacagcccg aaacacaacg ggttttcacc
     5101 gacaaagcag cttcatgcca cgccccaatc gtttttgccg atacaatata taatgcctgt
     5161 cggaagttga attcgggaaa atattctgtt tataccgtaa acagtgatat tacttaccac
     5221 ctcgaccttg gtggattgta tcagggtaaa aacctgcaaa caacattatg tgcacttgat
     5281 gaactgcgta aacactgtaa agccataaca aaagagcaca tatataaagc tctctcctca
     5341 gtacaaaaca ctacaggact acgcgggcgg tggcaagtgt tgtcggagaa tcctatggtt
     5401 gtttgcgaca cagggcataa tgctgaaggc atcagttaca caatgcagca gatagcgagc
     5461 cattcattcg aaaaactaca tatagttttc ggagtggtaa acgacaaaga cctgagcaaa
     5521 gttctgcagc ttttgcctaa agaagcaaca tactatttca caaaagcatc aatcccccgg
     5581 gctctcaatg aaaacgaact aaaattgata gcagaatcta ccggattgca cggggaagca
     5641 tatacaagtg tttctgaagc ctatgacaca gcccttcggc atgccggtaa gaaagacttt
     5701 atatacatcg gaggaagcac atttgtggta gcagaactgg aattagattg atttttttaa
     5761 taaaatattt tgcaggataa atattttatc tacatttgca ctctcaaaac cggagaaaag
     5821 ggagtttagc tcagctggtt cagagcatct gccttacaag cagagggtcg ggggttcgaa
     5881 tccctcaact cccacaagcc aagaaaacca ccgcgaaagc ggtggttttt gtttagtccc
     5941 ctgcgctcaa acttgtttga gagggcaagg ggactaaaca aaaactaggg acgcagtccc
     6001 gaagttttcg aaggcttgat tctcacccct aatagggatc annnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn ngtaatccct gaaagcaaaa ttctgtttat tctgcttgaa
     6181 aaaccaggga cgcagtcccg aagttttcga aggcttgatt ctcaccccta atagggatca
     6241 cgagcaaagc gagtaatccc tgaaagcaaa attctgttta ttctgcttga aaaaccaggg
     6301 acgcagttcc gaggttttcg aaggtttgat tttcccacca aatagggatc acgagcaaag
     6361 cgagtaattc caaatacaga ttactcctac acatgtatct tcttcgatga actccaatga
     6421 catttaatta cttctgagaa tacagaatgt agaatacagg aggggcgact gtcaattcaa
     6481 cagcattatt ctcttgctgg agttttcgtt ctctcccgta acatcatgcc tctatcgacc
     6541 aacgaaatgc ccccttctga attctatatt ctgtctcctg tattctgatt ccatattcct
     6601 ggtcttttca gattgctttt tttgccccaa taatgagccc tcataatggc aaggtgtcca
     6661 attacagact atctcgaaac ctgtctattc ttcgataaac tcagaatgac agggggagct
     6721 acctataatg tacttgtttc acatgtataa catacttgct gccaaaataa tattttgaaa
     6781 aacaaataat tttgagatta cgtaatccat tggtttttag attcatagaa aattattact
     6841 gaaaaatagt tttgaataaa atcacaaaac taattaagga atttcaatga taattgggta
     6901 tattcttaaa aaacattatt tttaatacga attttcgaaa taaataaaag taactataaa
     6961 tttacctaaa taaatttctg tctgtaattc aataatcagc gaatgaatca aaccaatcag
     7021 gcatggggaa gcagaatcgg gttaattcta gccatggcag gcaatgcagt cgggtttggc
     7081 aactttcttc ggtttccggt gcaggcaatt cagaatggcg gcggagcgtt tattatcccc
     7141 tatctcgtaa gttttttaat attggggttg cctttattgt ttattgagtg gagtattggt
     7201 cgttatgggg gcaaccgcgg ctttcacagc actcccctca tgatgcaaag tatggatcgc
     7261 agaaggatgt ggaaatacgt cggtgtattt ggtttgtttt gcaacatcgg gattgcctca
     7321 tattattgct acttggaatc atggacactt gcatatattt ttcacagtgc tgtaggcact
     7381 ttcaacggaa tgaaccagca tcaggtggtt cagttgttta gcgattatgt atcgattggc
     7441 aaaactacac ttataccctt tgagcccgtt gtattttttc taatctgcct cggcattaac
     7501 gtgtatattc tgagtaaagg tttaagcgga ggtgttgaaa aggttgctaa aataggaatg
     7561 ccattgctga tattatttgg tatcattctg gcaatcagag cagttacact gaaagcgggt
     7621 gtagatggtg cagtgaatga tagttcggtg gggctgaatt tcttatggac acctcatttc
     7681 gactcaatct ataaccctaa agtctggttt gcggcggcga gtcagatatt ctttaccctt
     7741 tcactgggta tggggtctat tcatgcttat gcctcttacc tgaaacgaaa agacgatatt
     7801 gccctgaatg ccatgtcggc aggatggatg aacgaatttg tagaggtagt tcttggtggt
     7861 gctattatta ttcctattgc tgtcggctat ttaggcatcg atcgtgttaa agaacttgca
     7921 acacttggtg gacttggtct tggttttaag acactaccgt atctctttgc acagtgggga
     7981 catgtatttg gagccttctg tggtgtgtta tggttcggac ttctattctt tgccggtatc
     8041 acgtcttcac tggcaatggg cacgcctgtg cagggctttt tagaagacga attcaaatgg
     8101 tcacgtaaaa aaactgccct cgctttcggc cttattatac ttgttttagg tttaccaacc
     8161 atcttgtttt tcaatcaggg ggtatttgat gaatatgatt attggacagg atcattcgcc
     8221 ttatttgtat ttgctgccct cgagtcgatt ttatttgctt ggttctttgg gattaaaaag
     8281 ggttgggccg aaataaacta cggggccgat attaaagtga gcaggatttt ccgatttcta
     8341 attgcttacg taacgcctgt actgctaata attgtttttt taggggcagt atttacacca
     8401 gcaggaaacg actgggctgc ggcttttcaa ggtctatttc atggccaggg ttggacgttt
     8461 gaccatggaa gtgtaattgg taagctcatg aacagtgggt tgaaagaaca actttctgcg
     8521 gctaccgatc aagtggttat agctgaattg aagtatagga tattgctaac caatttgtcg
     8581 agaatgcttc tggttatatt atttgttgca atatcgcttg ttgttcgaaa agcttattat
     8641 aaacataaaa gagaggggac tttataatga atacttcagc acttatttta atgctctgtt
     8701 caatttgcgg tgttaccgca gtaacagtgt atttcttcat caaagttcta acaaccaaac
     8761 ctaaacagga gcccgattct tataccgaca acgatgaggt gccacgataa atggcattta
     8821 attgtattga gttgctttta aatatttaag aatattattt gttaaggctt tttaagcttt
     8881 accttaagat atttttcagt attcaaccag aaataatcac ataaatattc ttcataaaag
     8941 aaatttctat tttgttcaaa aaactgtttt ttgtataaaa tattctatat ttttgtaatg
     9001 aatgttttta atatcactaa aatcattttc tatgaaatcg agtctgataa aaaaacacca
     9061 acagagatga aaatattttt atgcgaggtt ttatttgacc aataaaataa tttatataca
     9121 catgaaagca cattacttat tagttttcat attcatagtt gcccttatac caatggtttc
     9181 ggcccagcaa attattcggc aaactatatg cagtgttggg atgaatacaa attcaggtaa
     9241 ctttgtctta aagcaaacga tagggcagcc ttctaatact tcgactttta agaccgaaag
     9301 tggaatgctc cgtcaaggat ttcagcaacc tgctcatatt aacagagcaa ttgtacatta
     9361 cgaaaaccca atcgagatgc aactgtatcc gaatcctgct gaaaatagtt tcaccttgtt
     9421 aatcgttggg gacgatttgc gtttttctgc agtggcactt gatatcactg gtaaaactct
     9481 cctcaatatg aatatgattc tcaatcagaa aacggttgtt gattgctcag gctgggcacc
     9541 aggattgtat tttataaata tctattccga ttcccgctta attgcatcga aaaaaattgt
     9601 aaaaactata taacacatga aaaatacatt tatttctatt ctgttctgtc ttatagcaag
     9661 ctacagctta gctcaggata atgtcggttt gaattttgcg caaaatttta cgagttttcg
     9721 cttcatcgat tcccagggaa attttgatga aaatatgtca tctgacattc gtttttcgta
     9781 tggagtaaac tataataaga tattcaacaa gggtatattt attcgccccg agattggata
     9841 caaaaacttc ggagcaacat cggtaatgaa tgagaactac aaaatgtcgt ggaacctgca
     9901 ttatgccgat ataaatgccg gagcagggta tattttcaac aaatttgcat tgaaacccta
     9961 tattggggca tctttttact tctcgtattt gttcaaagca gaacagatga tgggtcaata
    10021 ctattacaat atttggagca gtggcgaaat taaaaaaaca gattttggaa tcaatgcctt
    10081 tgcaggagtg gtgtttgcgt tttcgaaaac agcatcagtt ttcttagaat tcagaaacac
    10141 aacaggatta caacagttag aacttaatag ttacgaaggg caaaatcaaa agctgttcaa
    10201 cagggctttt tcgcttcaat ttggattgca atttggcatt ccacaaaatg aaaagagatt
    10261 tggagtttaa tttatttcga aattataaat acagtaaata tgagaaagtt taaaaacata
    10321 gtaattgcag caatagcatg ttttatcagc agcttttctt atggtcagat tgttgttgat
    10381 ggtattacat atcaggctgt ggcagtcgac gtaaacggaa gagaaattgc aggtatggat
    10441 gcttatggta atatcgtggc caatcaagct atatggctca gattctccat tcttgaacaa
    10501 agcattgcag gtcctgtggt gtatcaggaa attgtgcaag tgaatactga tattaatggt
    10561 ttattcacac atgtaattgg tcttggtgct ataactgacg aaagtcccag caatagcatt
    10621 cttgatattg actggggcat tgaaaaacat tttcttaaag tagaaatgga cgatgtcccc
    10681 tacgataatt atgttttagc cggaattcaa caaatgatgg cagtacccta tgctttcttt
    10741 gcccttaatg ttaaaggtat tgatgcaaca gatatcgaaa attggaatac agcttattat
    10801 tggggaaatc atgccgatgt aggttatcta accagctata ccgaaaccga tcccattttt
    10861 ggcagcagcc ccgcttttgt aatctctact tcggatattg caaactggaa ttcagcgttt
    10921 tcgtggggaa atcatgccga tgtaggatac ttgaccagct ataccgaaac cgatccactt
    10981 tggacagcca gcccttcgtt tgagattacc aatgagaata tcaaccaatg gaattcttct
    11041 ttattaactt taaatggtat aactgcaacc acacaactgt tatcaaccag taccgatggt
    11101 ttttttctcg aatggataag tgaaggtgca actcataagt tgaatattcc gttagcctca
    11161 aattctggag tagatgcagg tttgattagc aatagcgatt ataattcatt taatgataaa
    11221 gtttcaggta gtggagtatt tcagcaaatc ccattttggt gggatcaaaa tcaacttggt
    11281 ggtgaccctg gtttaacatt taatgctgac gataactgcc ttcgaattgg agatgttgag
    11341 gaaaatatcg ctggaagccg tttgattatt aaaacattaa atcctgtttc aagtgcaaat
    11401 ggaatcacaa atattattga aagccataac gaagaagatg ctcaaggatt tggaatcaat
    11461 aattttttaa attgttataa tgcaacaaat aatggcatgc aagttggtat aagaaacatg
    11521 atttacaaag aaggttcagg tgggggctat ggtatttaca atagcattac cgactttggt
    11581 aatggcgatt cttatgccgg ttactttgca tcatacaaga attccatcga tggaattaac
    11641 tatggagtat attcactggt agagggaggg acaatcaact gggccggtta ttttgttgga
    11701 aacctatttg cacagggaaa tgttggcata ggaacaaata caccaacctc gaaacttagc
    11761 attaatggtg gtgatataaa tattttacaa atcgggtccg gtattattat gaaatcacct
    11821 gacgggcaat gctggagagt tacaatagac aatggcggaa actttgtaag tactgctatt
    11881 acctgcccat aagtatatcc tttgttaggt ttgatccaaa tatgccattt tattatcaca
    11941 atgtttatta aatggcaatt attccggtgg taacttatat tttttccatc tttgcaccga
    12001 aaaattgtac aaaataattt aaagtttcgt tctcgttatg ctgttgccta ccgaaataca
    12061 atgcttgtcg aattgcaaac tttgtcctcg tgaatgtggt gccaacagag ttttggaaaa
    12121 aacagggttc tgtaagactg atgcattatt tcgtgtttct gctgtatgtg cacacaaagg
    12181 cgaagaaagt gtaatttcgg ggagcaaagg aatatgcaat gttttttttc cacattgtaa
    12241 ccttcaatgt gtttattgtc agaatgccga tatttcggct aataatatcg ataatttacc
    12301 tgaagcccaa atatttgata agcttatcac gaaaatatgc tctgtacttg ataccaccga
    12361 aaatatagtg ggttttgtct cgccatcgca ttatgttatg caaatgaaag ctattattag
    12421 aggtattctt gaaactggtc gtaagccggt atttgtttat aataccaatg catacgataa
    12481 gcccgatact cttaagatgc ttgaaggtat aatagatgta tatctgcccg attttaaata
    12541 cacgaattca tccactgcat ccatgtactc gcaggcaact gattatccga aagctgcttt
    12601 ggctgctatt aaagagatgt accgacaaaa aggttcgact ctcattgttg atgataaagg
    12661 cattgtgcaa tcggggatta ttttgcgtca tttggttttg cccgggatgg ttaatcaaag
    12721 tatcgaagct cttaaatgta ttgccgaaga aatttctcca aaaattcacc tttctcttat
    12781 gtcgcaatat tacccggctc attttgcgaa aaattatccc gaaattaatc gcagcttatt
    12841 cgaagaagaa tataaagatg tgttggaagc gtgttacagc ttgggattct acaggggctg
    12901 gatgcaagat ttggaaagta ctaaaatatt ccgacctgat ttttcggcag aatctccttt
    12961 taatgagtga atgtcccgtg tgctttgatt ttacaaacgc gttggctatg taaaacaaaa
    13021 aaccgctata taagcggttt ttttgtttaa tataggatgt ttagttttta acagcccgtt
    13081 tcaccataac ctgatttccg ttttcgatgc ggaacatata tatcgaagga gcccagtcgc
    13141 tgatgtccat ctgccagaag aagctgtctg tttctgtttc cagatccaat cgataaatca
    13201 gacttccggt aagattgtat acttcaatca caacaggtcc ggcactgcaa tcttccaaat
    13261 aaatattcag ttcgttgcgg aacgggttgg gatacagatt catcgaagcc aggacttcag
    13321 tgtttggttc gggtgtttgt tcactctcat ccggcatatt gacatcttca atcggagcct
    13381 tagtaagtgg gtcgacaacc tcaagtgtag caatattgct tataatggca ttacaattca
    13441 aaccattgct ggtaacccat accctataac ttcttgtgta aggagggttt gccagtaagc
    13501 tggttccgac ggctcccggg atatcctgcc aaata
//
LOCUS       DAOOQP010000081        13434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_5653_length_13434_cov_6.545183, whole genome shotgun sequence.
ACCESSION   DAOOQP010000081 DAOOQP010000000
VERSION     DAOOQP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_13434_cov_6.545183"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLK75_08945"
     CDS             complement(<1..1206)
                     /locus_tag="PLK75_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39411.1"
                     /translation="MSPVNPIANQVIPNTNIDFSWNMHPDATVYHITIASNNDLTAII
                     YEDDVVINNKNYTITGQDTCYWNIVAFNGLEPVMLSDTFMFVIFTPLTFPGIQVWLNG
                     DDVEADGNNKVATWTDKSDNANSFAQDDDSKKPILITNGLNDLPALQFDGNDFLSGGD
                     ILDIGNASSWSLFVVGKSNASEGTFIAKSLAAGVPNRWALFYEGGSLTFLLSDQNGQA
                     FPFAAALSQYHLTTIHANRTGQNLKYRIDGVLKTSNNSISSNTYNLDSPYRCLLGGYS
                     NGSDNGEIYYLNGNISELIIYSSLSEDQSLFVENMLRYKYYPTLYKEPVSLGFDILIA
                     DNFCDTTLDAGIGFSDYLWSTGETTQTISVNQKGYYSVTATDSYGVTSSDIIYVSYPE
                     IGYSFADTTI"
     gene            complement(1446..1982)
                     /locus_tag="PLK75_08950"
     CDS             complement(1446..1982)
                     /locus_tag="PLK75_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY39412.1"
                     /translation="MTSFYSEHELQELGLHSVGKGVLISRKSSFYSPESIRIGNNVRI
                     DDFCILSGDITIGSHIHISAYCALYGAFGITLHDFTGMSPRSTIYSAMDNFSGDFLIG
                     PIHPIEKTDVTGGRVIMKQFSHLGACSTIFPNIVIEEGAVIGAYSLVNTNIPAWKIYC
                     GIPAKYLKDRSTNLLNLG"
     gene            2296..3768
                     /locus_tag="PLK75_08955"
     CDS             2296..3768
                     /locus_tag="PLK75_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOY39413.1"
                     /translation="MESLQKKSFKAFYWDFSGKIAGQLVAFIVSIFLARLLSPADFGL
                     LAIVNVIIAFSNIFLDLGLSVSLVQNANIREEHYGSVFWFNLATGILFTLLLFFTAGP
                     LAVFYENTEIKDITRLMSLLFLINAFGRVISAKLHKEMKFDVPVKAQLGAAFFSGVLG
                     VAMAFAGFGVWSLVAQSLLFAVLSNLLLYLLEKWRPKLYFSWKALKELWGFGFRMFLS
                     GVLNALYTNLDALIIGKLFPAAVLGQYYRAKSTSNIIVNNASGSLMAVFLPALSSVQN
                     DLERVRQIVSKSFHFICLISFFLTGFFYVIGGDCIIILFSDKWMPAVPLFELTMLSAY
                     AYPVSAVLVNVIVAMGNSKAFLRLEIYKKVLLSLNFIIGFYFGIEEFLIGFIIVNLLS
                     VLLNIWYAADDMKVPSKWFISRSAIYILSTFAITVTCKFAFMFFETNYVLHLLLCGSA
                     FCLLFLAAMLLFRSQGMRYVLEQLKWKNIGSRIIQRFYDR"
     gene            3758..4897
                     /locus_tag="PLK75_08960"
     CDS             3758..4897
                     /locus_tag="PLK75_08960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOY39414.1"
                     /translation="MTDKKKIYVTRPALPPLEEFEIYLKQMWETGWLTNNGPFHIELE
                     NKLRDFLQVDHISLFSNGTIALITALQALDLKGEIITTPYSFVATSHAIKWNGLTPVF
                     ADIDDNTCNLDPRSVEKQITQHTSAILPVHVYGIPCDIDALQKIADKYRLKIIYDAAH
                     AFNVKLQGRSILNFGDLTVLSFHATKAFHTFEGGAIVSHSAEQKAKIDFLKNFGFADE
                     LTIVGVGINGKMNEVQASMGLLQLKYFNNYVEQRKQISSMYLKKLNKTPGIRIPIIDK
                     NLDYNYSYFPVFINSQQFGNTRDGVYNSLKDNDIYARRYFYPLISNLPSYRSVVSASP
                     ENLPVANRIADVVLCLPLYPGLSEQEISEICEIIQNCYHPNIKNN"
     gene            4947..5963
                     /locus_tag="PLK75_08965"
     CDS             4947..5963
                     /locus_tag="PLK75_08965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39415.1"
                     /translation="MEENLLSTKIDFASEPNKPMLSVMMITYNHEKYIGQAIKSILQQ
                     TTDFEFEIVIGEDCSTDKTRSIICEIALEYPDKFKLMLHDKNLGMMQNFFTIIAACRG
                     KYVAICEGDDYWIDNGKLQKQVNFLEQNPDVSICCHNVFTEEEDGNRAVLWEWKEMQR
                     FSLYDLAKGNFISTPSVVFRRTPFSILPEKYLQAPLGDFILHLFNARAGDICYMPDIM
                     AVYRIHSGGVWTTRKSDIDKQINLYTKYLHTNQMLLEDFSDIRICRISFSKGTSESLN
                     QLRILSAKKGRIKESKQFARKLIHHMMSFGLVNARVLISCVQNLYFFKRFERNIYLHE
                     KGSE"
     gene            5944..7155
                     /locus_tag="PLK75_08970"
     CDS             5944..7155
                     /locus_tag="PLK75_08970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY39416.1"
                     /translation="MKKAVSNIILLFTNYLMLISVIISIPFLLLLKLIVRKSGFLYLE
                     NFPEENAGYQYRAAKWAQVLCQNSFKVKIKTVISEKKRFDNTGHFYYLWISYFKKFWH
                     IASSVRYKRVIVRREIMAANDYGYLFMERFLLVLHPNAILDFDDDLAYAKNQPKNNLS
                     FFGKIMLEHRDKFNASLRLYRYFIVGSPFLADLITSANPQLKRNAICYIPTCVDYDSI
                     EAKTYIAENDTIVLGWIGGIYNLYLLESLIPSLNRIAEKHKIKLVVIAGKQPEWEASF
                     EIQFLQWSLKNETDFLKSIDIGLMPLQNNIESIGKCGFKLIQYMGLGIPAIATNIGIN
                     TLIVEDGVNGFLVQPDADWYPVLDKAIKEKTRLSGMGRQARNTIEKEYSFKANAGKYM
                     EFVVPDYNSKE"
     gene            7227..9059
                     /gene="asnB"
                     /locus_tag="PLK75_08975"
     CDS             7227..9059
                     /gene="asnB"
                     /locus_tag="PLK75_08975"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY39417.1"
                     /translation="MCGIAGIVNFNKKVLETDVRRMCDTLIHRGPDAAGVWINHSSDV
                     GFGHRRLSIIDLSAAANQPMHYSGKFTITFNGEIFNYIELREELTKKGMQFRTDSDTE
                     VLLAAYEVYGENMLQHLDGQFAFAIYDNRNESVFCARDRIGEKPFYYYYDEDKGFYFA
                     SEMKAIFAAGIPCDVNQSMVYNYLLHWTLENPFNKAETFYKNIFSLEPASMLILNRHG
                     LKKRMYWQLNPNYTNHKISFQDAMQEYRFLFGESVRCRQRSDVPVGTSLSGGIDSSSV
                     VREMNSQLKDGTGQFCFSARFEEAGYDEGGFMSLATQGLNNVEWRQIFIDENDYWNNL
                     QKIAYHQEEPFLGPSVFVLWQVQKLAHDNGIVVLLDGQGADEILAGYFKYFNTFLRET
                     LFTRPLNFRNAKNNIFQKTGYQSGITALLTERMRNSASRTSFKGKRIKQLEDKGVLVD
                     TNAAANALQNPFTAFSKLNEALCFDVCFYGLGKLLRFADRSSMAFSREIRLPFLSHKL
                     IEFVFSLPADFKIHDGYTKYIHRKSMESVLPSEICWRTDKMSPQAPYDKWLNSSRGKE
                     MVQHYRHLLIESGCFSKDINISNFEIIDLGAFFENARALASHQN"
     gene            9071..10081
                     /locus_tag="PLK75_08980"
     CDS             9071..10081
                     /locus_tag="PLK75_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39418.1"
                     /translation="MKTKKDKYLLLKGVAGIGDRLHILAAAIDYCERNNRKIIVDWAD
                     GVFAAPGTDLFEKYFTCLHPLYCKQKPSANELTEMSFYPDIFRSFIYDDIYNHYTPVH
                     PGFIARFPRRFIPDGKWCKIYGYWCYNSNQIYKALGILLNINNKHDILQGRYLPDDIR
                     SDVVVYADMANISRKETFLKYIQIKDLIKSQIEKFVAEANLSEKSIGLHIRNTDKKPL
                     NSTATLIEHIKRKFGSDYTIFVATDNKNTEEEIKAHFTNVVKFDKFLPEVHIGGVHHY
                     AQQTKNWEKAELVLEQSIIDMWLLAKCEYLYYLGNSSFSVISSYLHNNAAKQTDWNML
                     QQ"
     gene            10092..10895
                     /locus_tag="PLK75_08985"
     CDS             10092..10895
                     /locus_tag="PLK75_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39419.1"
                     /translation="MKNTLITFLKNLFALAGFDYHRLMDRESVWQLIRKLRPWNTGTA
                     IIRLGPDGDGGYLVPDDLSGIEACFSPGVSNISGFEEDCAARGMLLFMADKSVERPNI
                     QLPEGKYQFTKKFIGHFNSDDYITMDEWVSSSLKNNNSDLLLQMDIESCEYLSFINMS
                     QKLLQRFRIIVVEFHQLDRLWNRDFYKLAEATFDKILLTHTCVHIHPNNRYSLHRRKG
                     IEIPVLAEFTFIRNDRITQKSPKTVFPDPLDFDNTTGKHIALPTCWYGE"
     gene            complement(10922..11482)
                     /locus_tag="PLK75_08990"
     CDS             complement(10922..11482)
                     /locus_tag="PLK75_08990"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOY39420.1"
                     /translation="MDDTLQKTLETLQHGGTILYPTDTVWGIGCDATNPNACKKLIQI
                     KPRDPSKSFIILVDSLVMLEKYVSRVPEIAYELIEVTTSPLTIIYPNACSLPEIVMSS
                     DESVGIRLVKSGFVKELISAFRKPIVSTSANFPGTPAPLEYSEIDHEIMKKVDYAVPF
                     EKEENTTQKESAIIKLFSDGSFKMIR"
     gene            complement(11491..12609)
                     /locus_tag="PLK75_08995"
     CDS             complement(11491..12609)
                     /locus_tag="PLK75_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOY39421.1"
                     /translation="MRIGFDAKRAFFNTSGLGNYSRNCIDLLRKNSPETELVLFTPRV
                     PNSLPEFATHPSIEVVYPNHGGKITEWLWRTHGMSKSASKCNIDLYHGLSNEIPLGLN
                     KRNIPCVVTIHDLIFLRYPELYRSVDRKIYHAKSLASCKYADAIIAVSKQTKEDIINF
                     YGIKPQKIEIIYQNCNPKFAEKISESEMQTVKDKYKIPENYILFVGTIEERKNVITIL
                     KAMQRFGIDKPLLLVGKATPYVNTIIGYAENNHIEDKLFIRHQIPDADMPAIYQMADV
                     FVYPSIFEGFGIPVLEAITSGVPVITSNISSLPEAAGPHSILTDPASVEMLGKSIIKV
                     LNDSDLRANMIGMGYQHAQLFSEKAIAEKLINLYHSLI"
     gene            complement(12620..13426)
                     /locus_tag="PLK75_09000"
     CDS             complement(12620..13426)
                     /locus_tag="PLK75_09000"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOY39422.1"
                     /translation="MQQLIESAYEDYRLLETNACTNAIQSAIAFLDTGKIRVAENIAG
                     QWKINEWIKKAVLLFFRIQKMEVIDTGYFEFYDKIPVKKGLSKLGVRVVPQAVCRYGC
                     FVAPGVVLMPSYVNIGAYIDEYTMVDTWATVGSCAQIGKNVHLSGGVGIGSVLEPLQA
                     SPVIIEDNCFIGSRCIVVEGVHVGKEAVLGANVVITSSTKIIDVSGSTSHEYTGYIPD
                     QSVVIPGTINKKFSAGEYQVPCALIIGKRKASTDKKTSLNQVLRDFEIAV"
BASE COUNT         3958 a         2581 c         2750 g         4145 t
ORIGIN      
        1 aatggtagta tcggcaaaag agtagcctat ttcggggtac gatacataaa taatatcgct
       61 tgaagtaaca ccataagagt cggttgcagt aaccgaataa taccctttct gatttaccga
      121 aatcgtctgc gttgtttctc ctgtactcca aagatagtcc gaaaatccga ttccggcatc
      181 aagggtggtg tcgcagaaat tatcagcaat taaaatatcg aacccaagac tcaccggctc
      241 cttgtataat gtggggtagt atttataacg cagcatattt tcaacaaaaa gcgattgatc
      301 ttccgacaga gaactataga tgattagttc gctgatattt ccgttaaggt aatatatttc
      361 cccattatca gaaccgttag aataccctcc caacaggcac ctgtaggggc tgtcaagatt
      421 atatgtattg ctcgaaattg agttgttgga ggttttgagc acaccgtcaa ttctatactt
      481 gaggttttgc cctgtgcggt ttgcatgaat ggttgtcaaa tgatactgcg ataaggctgc
      541 agcaaatggg aaagcctggc cgttctgatc cgataacaga aatgttaaac taccgccttc
      601 gtaaaacaga gcccatcggt tgggcacacc agctgcaagc gattttgcaa taaacgtacc
      661 ttctgaagca ttcgacttgc ctaccacgaa caatgaccat gaactggcat tcccaatgtc
      721 gagaatatcg ccaccactta aaaaatcgtt cccgtcgaac tgaagcgctg gcagatcgtt
      781 cagaccgttc gtaataagaa ttggtttttt tgaatcgtcg tcttgtgcaa aactgtttgc
      841 attatcagat ttatctgtcc atgttgccac tttattgttc ccatcagctt ctacatcatc
      901 accattgagc caaacctgaa ttcccgggaa agttaggggc gtaaagatta caaacataaa
      961 agtgtcggag agcatcacag gctctaatcc gttgaatgcc acgatattcc agtaacaagt
     1021 gtcttgccct gtaatggtat aatttttatt atttattaca acatcatcct catatataat
     1081 agcggtaagg tcgttgtttg acgcaattgt gatatgataa acagttgcat cggggtgcat
     1141 gttccatgaa aaatcgatgt ttgtatttgg aatcacctga tttgcaatgg gatttactgg
     1201 gctcaaaaac tgtgaataca gattatttat tgccagcact gatattagaa atataaaaaa
     1261 tgtaaatttt ctaagcataa ttggagacgt ttacacaaat gtaagttaat ttcaaataaa
     1321 tatgtataat ttatttgcat gaagtgtaaa tattttacag gattgtcttt agaaatttct
     1381 ctaagtttta aaaacctgtc agtgtaactg aatatgcatt tttaaattgg tctgattaca
     1441 acaaattaac ccagattcaa caaattagtg cttctgtctt tgaggtattt tgcgggaatt
     1501 ccacagtata ttttccatgc aggaatattg gtgttaacca gagaatatgc tccgattact
     1561 gctccttctt caatgacgat attcgggaaa atggttgagc atgcacccaa atgagaaaat
     1621 tgtttcatta ttacgcgtcc accggtaaca tcggtttttt caataggatg aataggccca
     1681 attaaaaagt cacccgaaaa attatccatt gcactatata tagtacttct gggcgacatg
     1741 ccggtaaaat cgtgtagcgt tatcccgaaa gctccatata gagcacaata ggctgaaatg
     1801 tgaatatggg aaccgattgt gatatccccc gataaaatgc aaaaatcatc aatcctaaca
     1861 ttattaccta tgcgtatgga ttccggggaa tagaagctac ttttcctgct tattagaacg
     1921 cctttcccaa cggaatgcaa cccaagttcc tgtaattcgt gttcgctgta gaatgaagtc
     1981 ataaattcgt gtgttactta tctgttaaaa gggttttgtt gtttttatcg ctaaaaattt
     2041 ttcatgtaaa attgataaaa tcaaagataa tattttctgc gatacttctc tgttttgaaa
     2101 atttcaacat gcgatacaaa tagatttacc ttatattacc cgtttaagta aatgattatt
     2161 ttgacgagaa aatatgccta ttattccatc tttacagcgg aacaattttc aatactttct
     2221 caacacagcg aattttgaaa agtaatcggg tatgaatgac tattttttat aatttaggca
     2281 tttaaatata cagcattgga aagccttcaa aagaaaagtt ttaaagcctt ctactgggat
     2341 ttcagcggaa aaattgcagg gcagctggtt gcttttattg tttccatttt tttggcacgt
     2401 ttacttagcc ccgccgattt tggcttactg gctattgtta acgtaattat tgctttttcc
     2461 aatatttttc tcgacctggg attgagtgtg tcgctggtgc aaaatgccaa tatcagagaa
     2521 gaacactatg gtagtgtttt ttggtttaac cttgcgaccg gcattttatt cacccttctg
     2581 ttatttttta ctgcagggcc tttggctgta ttttacgaaa acactgaaat taaggatatt
     2641 acccggctaa tgtcgctgct gttcctgata aatgcttttg gcagggtaat ttcagcaaag
     2701 cttcacaaag aaatgaaatt tgatgttcct gtgaaggcgc aattgggtgc tgcctttttc
     2761 agcggagttc ttggtgttgc aatggctttt gcaggttttg gcgtttggag ccttgttgcc
     2821 caaagtttat tatttgctgt attatcgaat cttttgctgt atctgcttga aaaatggagg
     2881 ccaaaactct atttctcgtg gaaagcactt aaagaactat ggggatttgg tttcagaatg
     2941 tttctttccg gagtgttgaa tgccttgtac acaaaccttg atgcactgat tatcggcaag
     3001 ctttttcctg ccgccgtttt agggcaatat taccgggcaa aatcaacttc aaatattatt
     3061 gttaataacg catccggaag ccttatggct gtattccttc cagcgctgag ttctgtacag
     3121 aacgatttgg aaagggtcag gcagattgta agcaaatcgt ttcactttat ttgcctgatc
     3181 tcgttttttc tcacgggctt tttttatgta attggaggcg actgtattat tattttattt
     3241 tccgacaaat ggatgcctgc cgttccgctt tttgaactga ccatgcttag tgcttatgct
     3301 tatccggtta gtgctgttct ggtgaatgtg attgtagcaa tgggtaattc aaaagcattt
     3361 ctgcgcctcg aaatttataa aaaagtactc ctgagcttga actttattat tggtttctat
     3421 tttggtatcg aagaattcct gattggtttt attattgtca acttactttc ggttttgttg
     3481 aatatctggt atgctgccga cgacatgaaa gttccgtcga aatggtttat ttcacgaagc
     3541 gcaatttata ttttaagtac ttttgccatt acggttacat gcaaatttgc atttatgttt
     3601 ttcgaaacaa attacgtatt gcatttatta ctttgcggta gtgcgttttg cttgttgttt
     3661 ttggcagcga tgcttttatt cagatcgcag ggaatgaggt atgttctcga gcaactgaaa
     3721 tggaaaaata ttgggagtag aataatacaa cggttttatg acagataaaa aaaagatata
     3781 cgttacccga ccggcattgc caccgcttga agagttcgaa atatatctga agcaaatgtg
     3841 ggaaacaggt tggcttacca acaatggccc attccatatt gaactcgaaa acaaattgcg
     3901 cgatttttta caggtcgatc atatatcctt gttctccaat ggtacgatag ctttgataac
     3961 agccttgcaa gccctcgacc tcaaaggaga aatcattaca acaccctaca gttttgtggc
     4021 aacttcgcat gccataaaat ggaacggact tacccctgtt tttgctgata tcgacgacaa
     4081 cacatgcaat cttgacccac gttcagtcga aaagcaaatt actcaacata cctctgcaat
     4141 tcttcctgtt catgtgtatg gtattccatg cgatatagat gccctgcaaa agattgccga
     4201 caagtatcga ctgaagatta tttacgatgc tgcgcatgct ttcaatgtaa agcttcaggg
     4261 gcgaagtatt ttgaattttg gagaccttac tgttttaagc tttcatgcta ccaaagcctt
     4321 tcatacgttt gagggcgggg ccatagtttc tcattcagca gaacagaaag caaaaatcga
     4381 ctttctgaaa aactttggat ttgccgatga gcttaccatt gtgggtgtgg gcattaacgg
     4441 taaaatgaac gaagtacagg cttcgatggg gttgttacag ctgaaatact tcaacaacta
     4501 tgttgaacag cgtaagcaga tatcgtcgat gtatttgaaa aaattgaaca aaactcccgg
     4561 aattagaatt ccgataattg ataaaaatct cgactataat tattcctatt tcccggtttt
     4621 tattaattca caacagtttg gcaacacaag ggatggtgtt tataattcgc tgaaagacaa
     4681 tgatatatat gccagaagat acttttatcc tcttatcagc aacctgccca gctatcgctc
     4741 agttgtttcg gcatctccag aaaatcttcc ggttgcaaat cgtattgcgg atgtagtgtt
     4801 atgtctgccc ttgtaccccg gattgagcga gcaagaaatt tcggagatct gcgaaataat
     4861 acaaaattgt taccacccta acatcaaaaa taattgacag ggcgtttcat taatattgtt
     4921 aaccggcttc tagcttcctg aaagatatgg aagagaacct attgtcaacg aaaatcgact
     4981 ttgcatcgga accgaataaa ccgatgctga gtgttatgat gattacctac aatcacgaaa
     5041 aatatattgg ccaggcaatc aaatcaattc tgcagcagac aaccgatttt gaatttgaaa
     5101 ttgtaatagg agaggactgc agcaccgata aaacaaggag tattatatgc gaaattgccc
     5161 tagaatatcc cgataagttc aaactaatgc tgcatgataa aaatttgggc atgatgcaga
     5221 atttttttac aataattgcg gcttgtcgag gtaaatatgt ggccatatgc gaaggcgatg
     5281 attattggat tgataatggc aaattgcaaa agcaggttaa ttttcttgag caaaatccgg
     5341 atgtttctat ttgttgccat aatgtattta ccgaagagga agacggcaat agagctgttt
     5401 tgtgggaatg gaaagaaatg cagagattca gcctctacga tttggcaaaa ggcaacttta
     5461 tttcaacgcc aagtgtagta tttcgcagaa cacctttctc gattttaccc gaaaaatatc
     5521 tgcaggctcc gttgggcgat ttcattttac acttgtttaa cgccagagcg ggcgatattt
     5581 gctatatgcc cgacattatg gcagtctatc gtattcacag cgggggtgtt tggactaccc
     5641 gaaaaagcga tattgataaa caaattaacc tgtacacaaa atatttacac accaaccaaa
     5701 tgcttctcga agatttttcc gacattcgta tttgccgcat aagcttcagt aagggtacct
     5761 cagaatcgct aaatcaactc aggattctat cggcgaaaaa aggaagaata aaagaatcga
     5821 aacaatttgc acgaaaactt atacaccaca tgatgagttt cgggcttgtc aacgcccgtg
     5881 ttttaatatc atgtgttcaa aatttgtatt tttttaaacg cttcgagcgt aatatttatt
     5941 tacatgaaaa aggcagtgag taatatcata ttattgttta ccaactattt gatgttgatt
     6001 tcagttatta tcagcatccc ctttctgctg cttttaaagt taattgttag aaaaagtggg
     6061 tttttgtatc tcgaaaattt tccggaagaa aatgccggtt atcagtatcg cgctgccaaa
     6121 tgggcgcaag tactttgtca gaactcgttt aaagtgaaaa ttaaaacagt tatttcagaa
     6181 aaaaaacggt ttgataatac agggcacttc tactatttat ggatttctta ttttaaaaag
     6241 ttttggcata tagcaagctc tgtgcgctat aaaagagtaa ttgtcaggcg cgaaattatg
     6301 gctgccaacg attatggata tttgtttatg gagaggttcc tattggtgtt acacccgaac
     6361 gccatcctcg attttgacga cgacctggca tatgcaaaaa atcaacccaa aaataatttg
     6421 agtttttttg gcaaaatcat gctggagcat cgcgataagt tcaatgccag ccttcgtctg
     6481 taccggtatt ttattgtggg gtctccattt cttgctgatt taatcacctc tgccaacccc
     6541 caactgaaac ggaatgctat ctgctatatt cctacctgcg tcgattacga cagtattgaa
     6601 gcaaaaacat atattgcaga aaacgatact atcgtgttgg gttggattgg tggtatctat
     6661 aatttgtact tgctcgaatc gcttattccc agcttgaatc gcattgcaga aaagcataaa
     6721 atcaaacttg ttgttattgc cggcaaacaa cccgaatggg aagcaagttt cgaaattcag
     6781 tttttgcaat ggtctttgaa aaatgaaacc gacttcctga aaagtattga catcggcttg
     6841 atgcctctgc aaaataacat tgagtctatc ggaaagtgcg gatttaagtt gattcaatat
     6901 atgggccttg gcattcctgc aatcgcaacc aatataggca tcaacacctt aattgtggag
     6961 gatggagtta atggttttct ggttcagcct gatgccgact ggtacccggt tctcgataaa
     7021 gccattaagg agaaaacaag gctttcggga atgggccggc aggctcgcaa tactatcgaa
     7081 aaagaatatt catttaaggc gaatgccggt aaatatatgg aatttgttgt gccggattat
     7141 aattctaaag aatagtaata ttgaaccatg cgcaattata tttttataag tttgtaaagg
     7201 gctaggttaa ttttttaaga acagttatgt gtggtatagc aggtattgta aattttaata
     7261 aaaaagttct tgaaacagac gtacgacgca tgtgcgatac tcttattcat cgcggtcctg
     7321 atgctgcagg tgtttggatt aaccacagct cagatgttgg tttcggacac aggcgtctga
     7381 gcatcattga cctttcggca gctgccaacc agccaatgca ttattccggc aagtttacca
     7441 ttacatttaa tggtgagatt ttcaattata tcgagcttcg cgaagagctt accaaaaaag
     7501 gaatgcagtt ccgtacagac agcgataccg aagtactgct tgctgcttat gaggtgtatg
     7561 gagagaatat gctgcaacac ctcgacgggc agtttgcttt tgcaatatac gataatcgca
     7621 atgagtctgt tttttgtgcc cgcgaccgta tcggcgaaaa accattttat tactactacg
     7681 acgaagacaa gggtttttac tttgcctcag agatgaaagc aatttttgca gccggaattc
     7741 cttgcgatgt aaatcaaagc atggtttata actatcttct tcactggaca ctggaaaacc
     7801 cttttaataa agcagaaacg ttttataaaa atattttttc actcgaaccg gcttcgatgc
     7861 tgatactgaa ccggcatgga ttgaaaaaaa gaatgtactg gcagctgaac ccgaattaca
     7921 caaatcataa gatatcgttt caggatgcaa tgcaagaata ccgattcctt ttcggggagt
     7981 cagtccgctg tcgtcaacgg tcggatgtgc ccgttggtac gtcgctttcg gggggaatcg
     8041 attcgagcag tgttgtacgg gaaatgaatt ctcagctcaa agatggtact ggccagtttt
     8101 gcttttccgc ccgtttcgag gaagcaggat acgatgaagg tggttttatg tcgcttgcga
     8161 ctcagggcct taacaacgtg gaatggaggc agatatttat cgatgaaaac gattactgga
     8221 acaacctgca gaaaattgcc tatcatcagg aggaaccttt tcttggcccc agtgtttttg
     8281 ttctctggca ggttcagaaa ctggcgcatg ataatggaat tgtagtatta ctcgacggac
     8341 agggagccga tgaaatactt gctggatatt ttaaatattt taatactttc ctcagggaaa
     8401 cgctttttac acgcccactc aactttagga atgcgaagaa caatattttt caaaaaacag
     8461 ggtaccagtc cggaattact gctcttttaa ctgaaagaat gagaaatagt gcttcccgca
     8521 cttcttttaa aggcaaacga ataaagcagt tagaagataa aggagtattg gtcgatacaa
     8581 atgcagcagc caatgcgctt cagaatccgt ttacagcttt ttcgaaactt aacgaagcac
     8641 tgtgctttga tgtgtgtttc tatggtcttg gaaaactgct gaggtttgcc gacaggagtt
     8701 ctatggcctt ctcacgggaa atcaggctgc catttttatc gcacaaactg attgaatttg
     8761 tgttttcgtt acccgctgat tttaaaatac acgatgggta taccaaatat attcaccgga
     8821 aaagcatgga atcggtgtta ccatcagaaa tttgctggag aaccgataaa atgagtccgc
     8881 aggcaccata cgacaaatgg ctgaattctt ccagaggaaa ggaaatggtg cagcactaca
     8941 gacatctgct gattgagtca ggatgctttt caaaggacat taacatatcc aatttcgaaa
     9001 ttattgacct gggtgcattt ttcgaaaatg cgagggcact tgcttcgcat caaaactaaa
     9061 gcattacaaa ttgaaaacga aaaaggataa atatctgttg ctaaaaggag ttgccggaat
     9121 tggcgaccgc ctacatatat tggcagcagc tatcgactat tgcgaacgaa acaaccggaa
     9181 aattattgtt gattgggcag atggcgtgtt cgctgctccg ggtaccgacc ttttcgaaaa
     9241 gtactttacc tgtctgcacc cattatattg taagcaaaaa ccatctgcaa acgaattgac
     9301 ggagatgagc ttttatcctg atatatttcg cagttttatt tacgacgata tttataatca
     9361 ctatactccg gtgcacccgg gcttcattgc gagattcccc cgacgattta ttcccgatgg
     9421 gaaatggtgt aaaatatatg ggtactggtg ctataatagc aatcagatat ataaagcctt
     9481 gggaatactg ttgaacatta acaataaaca cgatatcctt cagggaagat acctgcccga
     9541 tgacattcga agcgatgtgg ttgtgtatgc cgatatggcc aatatctcac gaaaagaaac
     9601 ttttttaaag tatattcaaa ttaaagattt gataaaatca caaatcgaaa aatttgttgc
     9661 tgaggcaaat ctgagtgaga aatcgattgg actgcacatt cgcaataccg ataaaaagcc
     9721 actgaactca acagcaacat tgatagaaca tattaaaaga aaatttggta gcgattatac
     9781 tatttttgtt gctaccgata ataaaaatac agaggaagaa attaaagcac attttacgaa
     9841 tgtcgtcaaa ttcgataaat ttttgccaga agtacatatt ggtggagttc accattatgc
     9901 gcaacagaca aaaaactggg aaaaggcaga attggtattg gaacaaagta ttatcgacat
     9961 gtggctttta gcgaaatgtg agtatttgta ttatctcggg aactcttctt tttcggttat
    10021 atcctcttat ttgcataata acgctgccaa acaaaccgat tggaatatgc tgcagcagta
    10081 acatcggaaa tatgaaaaac acattgatta catttttgaa aaatctgttt gcgctggccg
    10141 ggttcgacta tcacaggctt atggaccgtg aaagcgtttg gcagctgatt agaaaactaa
    10201 gaccatggaa tacgggtacc gcaattatcc ggcttggccc tgatggtgac ggtgggtatc
    10261 tggttccgga tgatttatcg ggaattgaag cctgcttctc gcccggtgta agcaatatca
    10321 gtggttttga ggaagactgt gccgcccgcg gtatgctgtt gttcatggcc gataaatctg
    10381 ttgaaaggcc taatattcag cttcctgagg ggaaatacca atttactaag aaatttatag
    10441 ggcacttcaa cagtgacgat tacatcacaa tggatgagtg ggtaagctca tctcttaaaa
    10501 acaacaactc cgatttattg cttcagatgg atattgaaag ttgcgaatac ctgagtttta
    10561 tcaatatgtc acagaaatta ctgcagcgat tcaggataat tgttgttgaa tttcaccagc
    10621 tcgacaggtt gtggaaccgc gatttttaca agcttgccga agcaactttc gataaaatat
    10681 tgcttacaca tacctgtgtg catattcatc caaacaatag gtattctttg cacagaagaa
    10741 agggcataga gattcctgtg cttgcagaat ttacttttat tcgcaacgac agaattacgc
    10801 agaaatctcc caaaactgtt tttcccgacc ctcttgattt tgataacaca actggaaaac
    10861 atattgccct tccaacttgc tggtatggtg aataggtttt taaaacccaa taggctctgt
    10921 attacctaat cattttaaaa cttccgtcag aaaacaactt aattatcgca gattcttttt
    10981 gggtcgtgtt ttcttctttt tcaaatggaa cagcgtagtc gaccttcttc atgatttcat
    11041 ggtcaatttc ggaatattcc aggggagcag gagtcccggg gaaatttgct gaagtactaa
    11101 caataggttt ccggaaagca ctgattaatt ccttgacaaa tccggatttt acaagcctga
    11161 ttcctacact ttcatcggac gacattacga tttcaggcag actacatgca ttgggataaa
    11221 ttatagtcaa aggacttgtg gtaacttcga taagttcata ggctatttca ggaactcttg
    11281 atacatattt ttcgagcatg accaaactgt ctacaagtat gataaaactc ttggaggggt
    11341 cgcggggctt gatttgaata agcttcttac aggcattcgg attggtggca tcacatccaa
    11401 taccccatac cgtatctgtg gggtataaaa ttgttccgcc atgttgcaat gtttccagag
    11461 ttttttgaag ggtgtcgtcc atgtactata ttaaattagc gaatggtaaa ggtttataag
    11521 tttttcagca atagctttct ctgaaaaaag ttgggcatgc tggtatccca ttccaatcat
    11581 gtttgctctt aagtcgctgt cgtttaaaac cttaataatt gacttgccaa gcatttcaac
    11641 cgatgcggga tcagtaagaa tgctgtgcgg tccggccgct tcaggtagac tgcttatgtt
    11701 gctggtgata acaggcacac cggaggtaat agcttcgagt acagggattc cgaacccttc
    11761 aaatatcgag gggtaaacaa atacatcagc catttgataa atagccggca tatctgcatc
    11821 aggaatctga tgccggataa atagcttgtc ctcaatatga ttattttcgg catacccaat
    11881 tatggtattt acataagggg tggccttgcc aaccaatagc aacggtttgt cgataccgaa
    11941 gcgctgcatg gcttttaata tcgtaattac gttttttcgc tcttcgatag taccaacgaa
    12001 caatatatag ttttcaggaa ttttatattt atcctttaca gtctgcattt cggattcgga
    12061 aattttttca gcaaattttg gattacagtt ctgatagata atttctattt tctgaggttt
    12121 tataccgtaa aagttaataa tgtcttcttt cgtttgtttg cttactgcaa taatagcatc
    12181 agcatattta cacgatgcca gcgatttggc gtggtatatt tttctgtcaa cagagcggta
    12241 tagttcagga tatcgtaaaa aaatgaggtc gtgaatagtt actacgcaag ggatgtttct
    12301 cttgttgagc ccaagtggta tttcgttact gagcccatgg tacaaatcga tattgcattt
    12361 cgatgctgat ttcgacatcc catgcgttct ccagagccac tcggtaattt ttcccccatg
    12421 gtttggatac actacctcaa tggaaggatg ggttgcaaat tcaggcagtg agttgggtac
    12481 acgtggtgtg aaaagcacaa gttcagtttc cggagaattc ttacgcagga ggtcaataca
    12541 gttgcggcta tagttaccca gcccgctcgt gttgaaaaat gcccgttttg catcaaagcc
    12601 tatccgcata actattatat taaactgcta tttcaaaatc gcgcaacact tgattcaatg
    12661 aggttttttt gtcggtagaa gcctttcttt tcccaataat caaggcgcag ggaacctgat
    12721 actctcctgc actaaacttt ttattaattg tgcccggaat aacaaccgac tgatcgggaa
    12781 tgtagcctgt gtattcatgg cttgtactgc ccgatacatc aataattttt gtgcttgatg
    12841 taatcactac atttgcaccc aatacggctt cttttccaac atgtacacct tcaacaacaa
    12901 tacaacggga tccgataaag caattgtctt caattatcac cgggctagcc tgcaagggtt
    12961 ctaatacact accgatacca acacctccgc tcagatgtac attttttcct atctgagcac
    13021 atgaacctac cgttgcccag gtatcaacca tcgtgtactc atcgatatag gcaccaatat
    13081 ttacatacga tggcatcagc actacgccgg gggctacaaa gcacccatag cggcaaactg
    13141 cttgtggaac cactcttacc cccaacttcg aaaggccttt ctttactggt attttgtcgt
    13201 agaactcaaa atacccggta tcgattactt ccattttttg tattctgaaa aacagaagaa
    13261 cagctttctt tatccattca ttaattttcc actgtcctgc aatattttcg gcaaccctaa
    13321 tcttacctgt gtcgagaaag gcaatagctg attgtattgc attggtacac gcatttgttt
    13381 cgagcaaccg ataatcttcg tatgcacttt caataagctg ttgtatgtgt tctg
//
LOCUS       DAOOQP010000082        13394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_5678_length_13394_cov_5.783042, whole genome shotgun sequence.
ACCESSION   DAOOQP010000082 DAOOQP010000000
VERSION     DAOOQP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_13394_cov_5.783042"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4147
                     /locus_tag="PLK75_09005"
     CDS             <1..4147
                     /locus_tag="PLK75_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39423.1"
                     /translation="KSNNKGLLIPRMNSTDRGNITNPAAGLLVYQTSAPAGFYYYDGT
                     SWTRISAGVSASGAINYVSKFTDTNSLGNSQIYDNGANVGIGTVSPGAKLDVVGPATG
                     AGPTIRALGGGDVVLNTGGSLFFDGNYAYGSGNYIRPVSSNTQAFFTGGAERMRITST
                     GNIGIGTGSPSYKLDVAGGAIRTDGQFISTLATGTSPLSVNSSTLVSNLNADLLDGYH
                     ASSFSTSSHTHAVLSQGTGIATFSYNGSAAQTVGLTGQALAFHNLATLGLVTLTSAGN
                     VTGRSITGTSNQITITNGNGVSGNPNVGINASFPEQIRGNVNITGGGTISYDASYNFR
                     WGTRFIIISNGRGAHFSIYGYYDIAIPAIGATITGVGGAANATVTAAGIPLTAWQALY
                     YIIVPGNSNTSVPANFRIVSYTSDLEVPESWILLAVHNGDDNSLRVCNGIILRPGQTW
                     SSGTSSITGGGTANYVPKWSSATTLTNSQIFDNGASVGVGIASPTEKLHVNGNIQSTG
                     YGIFPSGIAPASTGAPDAKIFSPSGQYLSNATWGINYNEGSPDYIEFLNANSVTSRIA
                     LDDGSAHLALGGGSVGIGKTNPAQKLDILGTSRFNDPSYVDTRYLEIWAGTAQFIRGT
                     NDLYIQPAGGMILQPGYPNLGTGNIQIKDGGAYLYGTFNGVNRTMDMSGNAGGLRFSA
                     NNWTDGGATYGHVISDNGTYKALMIVGNNITGNSGRGREVKVWDYLNVQGGFNVTGRF
                     YGVGTTDLVSNLNADLLDGQHGSYYAVASGSGNYIQNQTGADQSAGFRITGNGLFNGG
                     NVGIGVTSPGWKLDVNGNARIGANASQNTLAALAVSAGQGSATTYRDIDLHGSWSAGE
                     GHAITASHSTGSTNIVGQIVFQHDSPGSRIRFGRLYHSGDQSTYPMELVSNGSNAILR
                     LNTDSYTLYGPNSTWGAYLQVGGNGRVTTYASVAATNGNLHLDAADGSFATYVNYYSQ
                     NNTYINAQAGNVGIGNTGPSYKLDVTGDIRSTGNHIINNTSPTIYLQDSDNRSGMIHM
                     NSNLLYFLSANGTNSLTWAMNGSYWPLTINMTNDEFTFGGAAYFMEGNVGIGTTSPSY
                     KLHVNTTASGAAIYGYSSYSGSAIGGYFIGNYSGTSNPSYTYGIQAYGQGNGYQDSYG
                     VYGYASGPTSFRHGGYFSNSSGNYSYVAGYTNGAYKIYGSGSVSEIIPTENHGRVTLT
                     CPESPEYWYQDYGTVTMVNGKAHVELDPILVDIIVVDENNPLKVFCQVNIPDANDVAV
                     VNKTANGFDIIERNGGTHSGEIDYQIIAKPKTNFGQGRFIQAPGPGFLKAENDPAKAK
                     AANDGKQKIWTWPADWDVYNYDPANYCEPGKFVEGGKYDGYYKDEKGNLIPPDVYREM
                     FNK"
     gene            4174..5058
                     /locus_tag="PLK75_09010"
     CDS             4174..5058
                     /locus_tag="PLK75_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY39424.1"
                     /translation="MVKNTSLIFLLLSFAACVSYAQSSLSDTSYLKWCVYSLADDSMG
                     GRLAGSGYEEKAAIWIAQQLKENKINPFFKKNYYQGFEWTADSVNNISFNVIGTINNY
                     ADKTIIIGAHYDHVGMGGKRSRSYGKHNVHNGADDNASGVAAMLYVAAELRRNGSKNY
                     NYLFIAYGAHEPGLFGSQYFVDSNIVNPGSIRLLVNFDMIGRVSQSEPVLFWSSTDTT
                     LNLNNVTKDNNQMRCKEIDLPPGDHTAFADKKTPVLFLTTGVHDDYHKISDDADKINY
                     PGILGVCRIVIETISALK"
     gene            5154..7652
                     /locus_tag="PLK75_09015"
     CDS             5154..7652
                     /locus_tag="PLK75_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY39425.1"
                     /translation="MEVQTEINPQLLLAAEFVQNTHQNIFLTGKAGTGKTTFLRNLRE
                     SCPKRMAVLAPTGVAAINAGGVTIHSFFQLPFTPFIPEQQPASRPKNNTGESQEGFVH
                     KMTRNKIKLIRSLDLLVIDEISMVRADLLDAIDSMLRRYRYNQKPFGGLQLLMIGDLN
                     QLAPVVKEDEWRLLSEYYSNAYFFNSHALGKAGFTTIELKQIYRQEDQKFIELLGEIR
                     NNSMTQASFDLLNSRYIANFDPDESEGYITLTTHNAGAALINDKKMAALKSRSRKYKA
                     NITGDFQPYMFPTDEVLELKPGAQVMFVKNDSSPEKRYYNGKIGRLTRLDEDSVVVEC
                     KGDYDEIRVTPEEWSNVKLHLNEETREIEEEIIGSFKQIPLKLAWAITVHKSQGLTFE
                     KAVLDVNQAFASGQVYVALSRCRTLEGLVLISKIQRHAIKTDVNIREFNETALQNEPG
                     EDSLREAKAAFQQEVVFSIFDFSDLQRQFRSLKKHYQLNASKIHGADEAAFGRIEADA
                     VSEMYEVNDKFNIQLRSIIKQNPDLLPCDNPALQERISKAASYFLEKMASLFAIPMSS
                     IGFDSDNKEVKKEIIESAGRFELAMVLKTAALRAVTGGLDIQAYLRAVSDAEAGFVQT
                     LGLKKVPVENSVKSITHKKLYSLLHAWRASVADDLRVSPFMVMPFNTMKLICDKLPAT
                     QKELSKIKGLGKVKLKQFGSDILEMIHDYCLENAIERETPADDIPTKKKKEKKPESKS
                     VSLALFRENKSIPQIASERGFAESTIWGHLSQYVITGELQAEDLLDAGRIAEIKEFVG
                     NKRFSSIGQIMAECNGKFTFNELKIALSGNTLRD"
     gene            7891..8112
                     /locus_tag="PLK75_09020"
     CDS             7891..8112
                     /locus_tag="PLK75_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39426.1"
                     /translation="MKKIIKPVAGLVLFLVLNNMNLNAQGVAINLDGSVANASAMLDI
                     KSTDRGMLIPRISLSQTTSASPVSNPVVS"
     gene            8113..10794
                     /locus_tag="PLK75_09025"
     CDS             8113..10794
                     /locus_tag="PLK75_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39427.1"
                     /translation="MVYNTATANDVTPGYYHWDGSKWLRMFSGSPNDYILNQTASDQA
                     AGFRISGNGLIAGNVGIGTTSPSYKLDVNGNIRGTGTLYISTGNTTGGGIKLADDGDI
                     VDLNDGWATHRFSYGLRLTNANSSGATVIQLANGGSGYTYFNSGNVGIGTSTPQNTLD
                     VNGVINATTGYRVGNAATSGYYLRGNGTNFVSSPIQASDLPASSGNYIQNQYSGAQSA
                     NLWISGTANAATYTFTVPAGDPAPIITARTVPSGQGAANEKTELILFHTNDPANGSGV
                     DQITLRAPGLSFQTYNNATVGDINNNAGYNERMYINPDGNIGIGTTSPGSRVHISAGD
                     ASLTLYGPNASWSGKLYVGAAPNQNVASTAQVLSTDGNLHLDPAPSKNMYLGYYQARD
                     IYINPNGGNVGIGTTGPTSKLDVLGGDIRISSQGYGVYLNDETSGTNWQLHSHGDRLR
                     TYNGSLEYTYLTTADFTSGISGTTNYVAKFTGTNSIGNSVIFDNGTNVGIGTTSPQDK
                     LDVRDAMSVNEIKFRNVGGGDDSDPYRLRKVQGTSNTNWLELQLNDDSNEEFRIYGNS
                     CVGYGCAEYSGNLYHFFRADGTAYHSGNLGLGVMSPAYKLDVNGAANATNFWVGSGSY
                     ELTTSGWGGDRAIRIPAAANFRIYSDAGSTNLYVDGQYYANEGTALVWNAGNDGTGSG
                     SDADLLDGQHASSFLSTSNYGVGTVSADIAFSRDDISGWTTAAGSGVDDGAYLVNLGF
                     TITINGSSYTQCYLSTNGNIQFGSSGGTSYYYNTALPSTTFSLPTLCFYWDDMSTTGN
                     GIRYVTLGTTGNRVFIADFELITLSGSYYVTAQVQIHENSNLMNVRYYSVHPSACGDY
                     ATIGFQANTSQAIPVSCDAKVLDDNSNPESISFQIIK"
     gene            10833..11654
                     /locus_tag="PLK75_09030"
     CDS             10833..11654
                     /locus_tag="PLK75_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY39428.1"
                     /translation="MKQVIIGFIFLFSVQILIAQSKEYGTVGDQSLNDNFIKVIQIVY
                     PEFAFSPDAKSFESRIKKYYTVPKAAANRAREMGLDKSPQVQQELALLYQTIEEKYLS
                     ALYYNEINNSTQVTIEESKTYYNTHLSDFTKPAEYSYLVALVYDTTKNTIIKAGEKLN
                     YFAKSGNAQNDLKYGDKESFIIICQSNRVLTEQNREAAIIRSLNVGQISEPVLVNGML
                     SIFCVTGIKKEVVLPFDEVKEICAQQVKTIKINIATEQILEKAYEEYPVNMNTNK"
     gene            complement(11676..>13394)
                     /locus_tag="PLK75_09035"
     CDS             complement(11676..>13394)
                     /locus_tag="PLK75_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39429.1"
                     /translation="QWEWSINGEIVSNNQDFTHVFDSAGNYLIGLIVENSDGCSNSFN
                     EALLIHGLPVPDFSPDIACSGNLIVFTDNSISSEGVITSVWWNFDGTYNIEGTQVLHE
                     FVDSGEIPVTITVENSIGCTDSLTLFVEVLPSPVAAFTSTPACEGDLVYFINQSTSPF
                     PWEITNYNWLFGDGNSSAVSNPGYEYPEAGQYEVTLWVKALNGCSDTISEILTVNPLP
                     VADFNIPEFCLNRSQQLTDNSSVESGNIESWEYEVFWIGTYNIPNPEVSFPNVGDYPV
                     TLTVTTNAGCTDSETKTVSVYPLPTALFTSDVDWGAVPLEVAFSNQSIGAVDYEWRFG
                     DTGQSDIAEPTHTFADSGVYHVELIAVSEHGCTDTMRSSINVIVPILDLVLLNLETQI
                     ENDYLITSVDIINAGTLPLTEIDLIIHMPQNRSYRETVSGIFNPGSVTQFTLATKIYL
                     NSDIMPDYFCIEGVVTEYPEYADINPENQILCQSESENMIVSMPYPNPVNQILYLDIY
                     KTNGNSFDIEIYNSLGSKVYSETISDLSVRNYRIIYNLSDYPPGLYNIVIKQSDNIFS
                     RQFIKL"
BASE COUNT         4022 a         2696 c         3003 g         3673 t
ORIGIN      
        1 gaaatcgaac aacaaaggcc tgcttattcc acgaatgaac agcactgacc gaggcaatat
       61 taccaatccg gccgctgggc ttttagtgta tcaaacctct gcccctgctg gtttttatta
      121 ttacgatggg acatcatgga ctcgtatcag cgcaggagtt agtgcatcgg gagcaataaa
      181 ttatgtttca aagttcactg ataccaattc gcttggaaat tcacagattt acgataatgg
      241 tgccaatgtt gggattggca ctgttagtcc gggggctaag cttgacgtgg taggcccggc
      301 aacaggtgca ggtcctacaa ttcgggcctt gggaggtggc gatgtagtgc tgaacaccgg
      361 aggttcgttg ttttttgatg ggaattatgc atatggttca ggtaattata tccgacctgt
      421 ttcgagcaat acgcaggcat tttttaccgg aggcgctgaa cggatgcgta ttacttcaac
      481 aggaaatatc ggaattggaa ccggttctcc ttcgtataaa cttgatgttg caggaggagc
      541 tatcagaacc gacggtcagt ttatttctac tcttgctact ggtacatccc cactatcggt
      601 taactcatca acacttgttt cgaatcttaa tgccgattta ttagacgggt accatgcttc
      661 atcgttttct acctcgagtc atacccatgc agtactttct caggggacag gtatagcgac
      721 atttagttat aatggtagcg ctgcacaaac cgttggttta acaggtcaag cacttgcgtt
      781 ccacaattta gccacactgg gattggtaac cctcaccagt gccggaaatg ttactggcag
      841 aagcataaca gggacttcca atcagataac aattaccaat ggtaacgggg tctccggaaa
      901 cccaaatgtt ggaataaatg cttctttccc agagcagatt agaggcaatg ttaatattac
      961 aggaggtgga actatttcct acgatgcttc atataatttt cgctggggta cacgttttat
     1021 cattatttcc aatggcaggg gagctcactt ttcaatatac ggatactatg atattgcaat
     1081 acccgcaatt ggtgccacaa taactggtgt tgggggagca gcaaatgcaa ccgtaaccgc
     1141 ggccggtatt cctcttacag catggcaggc attatattac atcatagtac cgggaaattc
     1201 gaatacttct gtccctgcca acttcagaat agtatcctat accagcgatt tggaagtacc
     1261 tgagtcatgg atattactag ccgtacacaa tggagatgat aattctctca gggtttgcaa
     1321 tggaataatt cttcgcccgg gacaaacatg gtcatcggga acttcatcaa taactggagg
     1381 aggaacagcc aattatgttc ccaaatggag cagtgcgacg acacttacca attcgcagat
     1441 ttttgacaac ggagcttcag ttggtgtcgg aattgcaagc ccaacagaaa aacttcatgt
     1501 aaatgggaac attcaatcga cgggatatgg gatctttcca agcggaattg caccagcttc
     1561 tacgggggct ccggatgcta aaatattttc accatccggc cagtatttgt caaatgccac
     1621 atggggtatc aattacaatg aaggcagtcc tgattatatt gaattcctta acgcaaacag
     1681 tgtaacatca cgcatcgcat tggatgacgg aagtgcccat ttagcattgg gaggaggttc
     1741 ggtgggcatt ggaaaaacaa accctgctca aaaacttgat attcttggaa catcccgatt
     1801 taacgatcct tcgtatgttg ataccaggta tttggaaatt tgggccggta ctgcacaatt
     1861 cattcgtggc actaacgatc tttatattca gcctgccgga ggaatgattt tgcaaccagg
     1921 atacccgaat ttgggaacag gtaatattca aataaaagac ggtggcgcat acctttatgg
     1981 gacatttaac ggtgttaaca gaaccatgga tatgtcgggt aatgctggcg gacttcggtt
     2041 tagtgcaaat aactggactg atggaggggc aacctatggc catgtgatca gtgataacgg
     2101 cacttataaa gcgctgatga ttgtaggtaa caacattact ggtaacagcg gacgtgggcg
     2161 tgaagttaaa gtatgggatt acctgaatgt acagggtggt tttaatgtta ccggaaggtt
     2221 ttatggagtt ggtaccactg atcttgttag caatcttaat gctgatttgc ttgatggtca
     2281 gcatgggagt tattacgcag tggcatccgg tagtggaaac tatattcaga accagacagg
     2341 ggcagatcag tctgccggat tcagaattac cggtaatggt ttatttaatg gtggaaatgt
     2401 tgggattggc gttacaagcc cgggttggaa gctcgacgtg aatggcaatg caagaattgg
     2461 agcgaatgcc agccaaaata cattagctgc actagctgtg tctgccggac aggggagtgc
     2521 aacaacttat cgagatatcg atttgcatgg gtcgtggtct gccggtgagg gtcatgccat
     2581 tactgcatcg cattccacag gtagtacaaa catagtgggg caaattgtat ttcaacatga
     2641 cagcccaggt tcacgaattc gtttcggccg gctatatcat agcggagatc agtcgacata
     2701 tcctatggaa cttgtgagca acggatcaaa tgcaatactc agactcaata ccgatagcta
     2761 cacattatat ggaccaaatt caacatgggg ggcatatttg caggttggtg gcaatggcag
     2821 ggtaacaacc tatgcttcag tagcagcaac caacgggaat ctgcaccttg atgcagccga
     2881 tggaagtttt gcaacctatg tgaattatta tagccagaac aacacgtata tcaacgctca
     2941 ggcaggtaac gtcggcatcg ggaatacagg tccttcctat aaacttgatg ttaccggtga
     3001 tataaggtca accggaaatc atattattaa caatacttca ccaaccattt atctgcagga
     3061 tagcgataac cgttctggta tgattcacat gaattcaaat ttgctttact ttctttctgc
     3121 aaatggaacc aacagtttga catgggctat gaatggctcc tattggccac ttactattaa
     3181 tatgacgaat gatgaattta cattcggagg tgcggcctat tttatggaag gcaatgtggg
     3241 tattggaaca acaagcccga gttacaaatt acatgttaat actactgcat ctggagcagc
     3301 catatatggg tattcatcat attctggatc tgctataggt ggatatttta ttggtaatta
     3361 ctcaggcact tcaaatccta gttatacgta tggaattcaa gcatacggac aaggaaatgg
     3421 ttatcaggac tcttatggtg tatatggata tgcaagcggt cccacaagtt ttcgtcatgg
     3481 cggatatttt tcaaatagct caggtaatta ttcatatgtt gcaggttaca caaatggagc
     3541 atataagata tatggtagtg ggtcggtttc cgaaattata cccacggaaa atcatggaag
     3601 agttactttg acttgccctg aatctccaga atattggtat caggactacg gaacagtaac
     3661 aatggtcaac ggcaaagctc atgttgaact agaccccatc cttgttgaca ttattgttgt
     3721 tgacgagaat aatccgttga aagtattttg tcaggttaat attcctgatg caaacgatgt
     3781 tgctgttgta aataaaacag ctaatggctt cgatattatt gaacgaaacg gaggaacaca
     3841 tagcggcgaa attgattatc aaattattgc aaaacctaaa acaaactttg gtcaaggccg
     3901 atttattcaa gctccaggcc ccggattctt aaaagctgaa aacgatccgg caaaagcgaa
     3961 agccgcaaac gatggtaaac aaaaaatatg gacatggcct gcagattggg atgtatataa
     4021 ctatgatcca gccaattatt gtgagccggg taagtttgtt gaaggcggga aatatgatgg
     4081 ctactataaa gatgaaaaag gtaatttaat accaccggat gtataccgtg aaatgtttaa
     4141 caagtaattt ctaagcggct gaaaactcag cttatggtaa aaaatacaag cctcatattt
     4201 ctattgcttt catttgctgc ttgcgtatcg tatgctcaaa gttcacttag cgatacctcc
     4261 taccttaaat ggtgtgtgta ctcgcttgcc gatgatagta tgggaggtcg gctggccggg
     4321 tctggatacg aagaaaaagc cgcaatatgg attgctcagc aactaaaaga aaataaaatc
     4381 aatccttttt tcaaaaagaa ctactatcag ggttttgaat ggactgctga ttcggtgaat
     4441 aacatatcct tcaatgttat tggcactatt aataattatg ccgataaaac aattatcatt
     4501 ggtgctcatt acgaccacgt gggaatggga ggaaaacggt cgcggtcgta tgggaaacac
     4561 aatgttcaca atggggccga cgataatgcc agtggggtgg ccgcaatgct gtacgttgcg
     4621 gcagaattgc gccgcaacgg cagcaaaaat tataattacc tgttcatcgc ctacggtgca
     4681 catgaaccgg gtctcttcgg ttcacagtat tttgtcgata gcaatatcgt taatccgggt
     4741 tctatacgcc tgctggttaa cttcgatatg attggaaggg tgtcgcaatc agagcccgtt
     4801 ttgttctggt cgtctaccga taccacactg aacctgaata atgtgacgaa agataataat
     4861 caaatgagat gtaaagaaat tgatctgcca ccgggtgatc acaccgcctt tgccgacaaa
     4921 aaaacaccgg tcttatttct cacaacgggt gtacacgacg attatcacaa aataagtgat
     4981 gatgccgata aaatcaacta cccaggaatt ttaggagtct gccggattgt tatcgaaaca
     5041 atatctgcat taaaatagta aatcgcgccc actctattca ctctggtaaa aatattgggt
     5101 tcatttgcgt atattcgcta cgaattcatc aattttttga aaagacaatt gacatggaag
     5161 tccaaaccga aataaacccc cagctattgc tggccgctga gtttgtgcaa aatacccacc
     5221 aaaatatttt ccttaccgga aaagccggca ccggaaaaac cacattcctg cgtaatctcc
     5281 gggaaagctg tcccaaacgt atggctgtgc tggctcccac cggggtggct gctataaatg
     5341 ccggaggcgt aaccattcat tcgttttttc agttgccttt tactcctttt atacctgaac
     5401 agcaaccggc ctcccgcccg aaaaacaata ccggtgagtc gcaggaaggt tttgtacata
     5461 aaatgacccg taataaaatt aaactcatcc gcagcctcga tttgctggtt attgacgaaa
     5521 tcagtatggt gcgcgccgac ctgctcgatg ccatcgacag catgctgagg cgataccgat
     5581 ataatcagaa accattcgga ggactgcaac tgctgatgat aggcgattta aaccagttgg
     5641 ctcccgtcgt aaaagaagat gagtggaggc tgctgtcgga atattattcc aacgcatact
     5701 ttttcaacag ccacgccctg ggcaaagccg ggtttactac catcgaacta aagcaaatct
     5761 atcgccagga agaccaaaaa ttcattgaac tgctgggtga aatcagaaac aattcgatga
     5821 ctcaggcctc ttttgatttg ttaaattcga ggtatattgc aaactttgac cccgatgagt
     5881 cggaaggcta tattaccctc acgacccaca atgccggtgc agcgctgatt aacgacaaaa
     5941 aaatggccgc actgaaatcg cgttcacgca agtacaaggc taatattaca ggcgactttc
     6001 aaccctatat gtttcccacc gacgaggtgc tggagcttaa acccggggct caggtaatgt
     6061 ttgtaaaaaa tgactcttca cccgaaaaaa gatattataa tggtaaaatt ggccgcctaa
     6121 cccgtctaga cgaagactcg gtggtggttg aatgcaaagg tgactacgat gaaatcagag
     6181 taactcccga agaatggagc aatgtgaagc ttcacctcaa cgaagaaacg cgcgaaattg
     6241 aggaagaaat tataggcagc ttcaagcaaa ttccattgaa acttgcctgg gctattacgg
     6301 tgcacaaaag tcagggactt acattcgaaa aagccgttct cgatgtgaat caggcttttg
     6361 cctcaggtca ggtgtatgtt gccctcagcc gttgccgaac cctcgaagga ctggttctta
     6421 tttcgaagat acaacgccat gccataaaaa ccgatgtcaa catacgcgag ttcaacgaaa
     6481 cagccttgca aaacgagccc ggcgaagatt ccctgcgaga agcgaaagcc gcatttcagc
     6541 aggaagtcgt tttttcgata ttcgatttca gcgatttaca acgacagttc aggtcgttga
     6601 aaaaacacta tcagctcaat gcttccaaaa ttcacggtgc cgatgaagca gcttttgggc
     6661 gtatcgaagc agatgcagtt tcggaaatgt atgaggtgaa tgataaattt aacatccagt
     6721 tgcggtcaat cattaaacaa aatcccgacc tactgccctg cgataatccg gcattgcagg
     6781 aacgcatttc gaaagccgca agctattttc tcgaaaagat ggcgtcgctg tttgccattc
     6841 cgatgagtag catcggtttc gactccgaca acaaggaagt gaaaaaagaa atcatagaat
     6901 cagccggtcg cttcgagctg gctatggtgc taaaaaccgc agcactcagg gctgtaaccg
     6961 gaggacttga tatacaggct tacctcaggg cggtttccga tgctgaggcc ggctttgtgc
     7021 aaacgcttgg acttaaaaaa gtgcctgtcg aaaactcagt aaaatcaatc actcacaaaa
     7081 aactgtattc gctgcttcat gcatggcgtg cttcggttgc cgacgattta cgggtgtcgc
     7141 cgtttatggt aatgccattc aataccatga aactgatttg tgataaacta cccgcaacac
     7201 aaaaagaact atcaaaaata aagggactgg gcaaggtaaa acttaaacag tttggcagcg
     7261 acattctcga aatgatacat gattattgcc tcgaaaatgc cattgaacgg gaaactcctg
     7321 ccgacgatat tcctacaaaa aagaaaaaag aaaaaaagcc ggaatccaaa tcggtaagtc
     7381 tggcgctgtt ccgcgaaaac aaaagtattc cgcaaatcgc ttccgaaaga ggttttgccg
     7441 aatctaccat ctggggacac ctcagtcaat atgttatcac cggagaacta caggccgaag
     7501 acttactcga tgccggacgc attgccgaaa tcaaggaatt tgttggaaac aaaagattca
     7561 gtagtattgg tcaaataatg gctgagtgca atggcaaatt cactttcaat gaacttaaaa
     7621 tagcgctgtc aggcaatacc ttgcgggatt agtacaactt ttactgaatc agggtgttta
     7681 taataaacgg ttcttctgca tggcaattcc ctgttaaact tacacgcttg tttaatgcca
     7741 accaaatggg aaacatgatg caaccgttga aacgtttaag cacatagtgc aagtcattac
     7801 aaccggcata atcgttcact gtagttgatg aataataatt tctgtttgtt gcaaagaata
     7861 cactaatttt gcagtataaa tacaattgca atgaaaaaaa taataaaacc tgttgcaggt
     7921 cttgttttat ttcttgtttt aaataacatg aatcttaacg ctcagggcgt tgccattaac
     7981 cttgatggtt cagttgcgaa cgcatcggct atgcttgata ttaaatcaac agacaggggt
     8041 atgcttattc ccaggatatc tttgtcgcaa actacctctg ccagtcctgt ttcgaaccct
     8101 gttgttagtt agatggtgta caatactgca acagccaacg atgtaacgcc cggttattat
     8161 cattgggatg gcagcaaatg gttacgtatg ttttctggta gtccgaatga ttatatattg
     8221 aatcagacag catctgatca ggcagcagga tttcggatca gtggaaatgg attaattgca
     8281 gggaatgtgg gtatcggcac aaccagccct tcatataaac ttgatgtcaa cggtaatatt
     8341 cgaggtaccg gaaccctgta catcagcacc ggaaatacta ctggcggcgg gattaaactg
     8401 gctgatgatg gggatattgt tgacctcaat gatggttggg ctacccaccg attcagctat
     8461 ggattaaggt taactaatgc aaatagcagt ggtgccactg taattcaatt agcaaatggt
     8521 ggttctggtt atacatactt caatagtgga aatgttggta ttggtacttc tacccctcaa
     8581 aacacccttg atgtaaacgg agtaataaat gcaactaccg gatatagggt tggtaatgca
     8641 gccacaagtg gttattacct tagaggaaac ggtaccaatt ttgtttcatc gccaattcag
     8701 gctagcgatt tacctgcaag tagtggtaat tatattcaaa atcaatattc cggagctcaa
     8761 agtgctaacc tctggattag tggaacagcc aatgctgcaa cctatacttt tacggtccca
     8821 gctggcgatc ctgctccaat tattactgca cgcactgttc cttctggtca gggcgccgcg
     8881 aatgaaaaaa ccgagttgat attgtttcac accaacgacc ctgccaatgg ttcaggagtg
     8941 gatcagatca cactaagagc tcccggacta agcttccaga cctataataa tgctacggtt
     9001 ggcgatatta ataataatgc cggatataat gaaaggatgt acattaatcc cgatggaaat
     9061 attggcatcg gtacaacctc tccgggttca agagtacata tttcagcagg agatgcctcg
     9121 cttacgctgt atggacccaa tgcatcatgg agtggaaaac tatacgtagg ggccgctccc
     9181 aatcagaatg tagcctcaac agcgcaggtt ctctcaaccg atggtaattt gcatttggac
     9241 cccgctccat caaagaatat gtatttagga tattatcaag cacgagatat ttatattaat
     9301 ccaaatggtg gaaatgttgg tataggaaca actggtccaa cttcgaagct ggatgttttg
     9361 ggaggagata tacggatttc gtcacagggc tatggcgtgt atctgaacga tgaaacatcc
     9421 ggaaccaact ggcagttgca ttcgcatggc gacaggctga gaacgtataa tggttctttg
     9481 gaatatacat accttaccac agccgacttt acctcaggta tttcgggaac gaccaattat
     9541 gttgcaaaat ttactggaac caatagcatt gggaattctg ttattttcga taatggtaca
     9601 aatgtaggaa taggtaccac tagccctcag gataagcttg atgttcgcga tgccatgtcg
     9661 gtaaacgaga ttaaattccg taatgttggt ggtggcgatg attccgaccc ataccggtta
     9721 cgaaaagttc agggaacgag caatacaaac tggcttgaac tacagcttaa cgatgattca
     9781 aacgaggagt ttcgtattta tggcaactct tgcgtgggtt atggatgtgc agaatacagc
     9841 ggaaatttat atcatttttt cagagcggac ggaactgcct atcattcggg aaatttggga
     9901 ctgggagtga tgagcccagc ttataaactt gatgttaatg gcgcagcaaa tgctacaaat
     9961 ttctgggttg gaagtggcag ttacgaatta acaacgagcg gatggggagg cgacagggca
    10021 attcgcatac ctgctgcagc aaatttcaga atttattctg atgccggatc aacaaactta
    10081 tatgttgatg gtcaatatta tgccaacgaa ggaactgcat tggtatggaa tgccggtaat
    10141 gacggaactg gcagcggaag tgatgccgat ttattagacg gacaacatgc ttcgtcattt
    10201 ctgagtacaa gcaattatgg agtaggaact gtttcggccg atattgcctt cagccgtgat
    10261 gatattagcg gatggacaac ggccgctggc tccggtgttg atgatggagc ttatcttgtt
    10321 aatcttggtt ttaccataac aattaacggt tcatcctata cgcagtgtta cctttctacc
    10381 aatggcaata ttcagtttgg tagttcaggg ggaacttcgt actattataa tacggcatta
    10441 ccaagtacaa ctttttcgtt gccaacacta tgtttttatt gggatgatat gtcaacaaca
    10501 ggaaatggta ttagatacgt tactttagga actaccggaa acagagtctt tattgcagat
    10561 tttgagctta ttactttatc aggatcttat tatgttacgg cccaggtgca aatacacgaa
    10621 aattctaatt taatgaatgt acgctattac agtgtgcatc ccagcgcctg tggtgattat
    10681 gctacaatag gctttcaggc taacacatct caggcaatac cagtatcgtg tgatgcaaag
    10741 gttttggatg ataattcgaa tccggagtca atatcctttc aaatcattaa gtaaaatagg
    10801 tattgatagt attttaaatt aggggaattg gcatgaaaca agttatcata ggatttattt
    10861 ttcttttttc ggtacaaatc cttattgcgc agagcaagga atacggtact gttggggatc
    10921 agagtctcaa cgataatttt attaaagtaa ttcagattgt ttaccccgaa tttgcatttt
    10981 ctccggatgc gaaatctttt gaaagtcgta ttaaaaagta ttatactgtc cctaaagctg
    11041 cagcaaaccg tgccagagaa atggggctgg ataaaagtcc gcaggtgcag caggaacttg
    11101 cgcttcttta tcagacaatt gaggaaaaat atctgtctgc attgtactac aatgaaatca
    11161 ataatagtac acaagtaacc attgaagaaa gtaaaaccta ttataataca cacctgtctg
    11221 attttaccaa accggctgag tatagttatt tagtggctct tgtttacgat acaacaaaaa
    11281 atacaattat taaggcaggt gaaaagctga attattttgc taaatcaggt aatgcacaga
    11341 atgatttaaa atatggagac aaagagagtt ttataattat ttgtcaatcg aacagggttt
    11401 taacagagca aaatcgtgaa gcagcaatta tacgttcgtt aaatgtcgga caaatcagtg
    11461 agccggtgtt ggtgaatggt atgctaagca ttttctgtgt aaccggtatt aaaaaagaag
    11521 ttgtgttgcc atttgatgaa gtaaaagaaa tatgtgctca gcaagtaaaa acaattaaga
    11581 ttaatattgc cacagaacaa attcttgaaa aagcatatga agaatatccg gtcaacatga
    11641 atacgaacaa ataattaatt ggcggtattt accaattaca attttataaa ctgccggctg
    11701 aaaatgttat cactctgttt tattacaata ttatatagcc ctggtggata atcgctaaga
    11761 ttatagatta tacggtagtt tcttaccgac aaatccgaaa tggtttcgga atacactttt
    11821 gatcccagcg aattgtaaat ctcgatatca aacgaattcc cattggtttt ataaatatca
    11881 aggtagagaa tttgattaac agggttcggg tatggcattg atacaatcat gttttccgac
    11941 tccgattgac acaaaatctg gttttcgggg tttatatcgg catattcagg gtattcggta
    12001 accactcctt cgatgcagaa ataatcaggc ataatgtcgc tgtttaaata gattttggtt
    12061 gctaatgtga actgggtaac agagcccgga ttgaaaatac ctgaaacagt ttcgcgatag
    12121 ctgcggtttt gtggcatgtg aattattaag tcgatttccg tcaggggtag tgtgccggca
    12181 tttataatat cgaccgaggt aatcaggtaa tcgttttcga tttgggtttc caaattgagc
    12241 agtaccaggt ctaaaatagg aacaatcaca tttattgaag atcgcatggt gtcggtgcag
    12301 ccatgctccg aaaccgcaat aagttcgaca tgatacactc ccgaatctgc aaatgtatgt
    12361 gttggttcag caatatcgct ttgcccagta tcaccaaaac gccattcata atctaccgcg
    12421 ccaatacttt gatttgagaa tgctacttcg agaggaacag caccccaatc aacgtcggaa
    12481 gtgaacaatg ccgtcggcag cggatatacc gaaaccgttt tggtttcaga atcggtgcat
    12541 cctgcattgg ttgtaactgt aagggttaca ggataatcgc caacatttgg aaaactgact
    12601 tccggatttg gtatattgta agttcctatc cagaaaactt catattccca actttcgata
    12661 ttgcccgatt ccacactgct gttgtcggtt aattgctgtg aacggtttaa acaaaattcc
    12721 ggaatattaa aatcggccac aggtaacgga tttacagtca gtatttctga aatggtatcg
    12781 ctacatccgt taagcgcctt tacccataag gtaacttcgt attgcccggc ttcgggatat
    12841 tcgtaaccgg ggtttgaaac cgccgaggaa tttccgtcgc caaatagcca gttataattt
    12901 gtaatttccc aggggaacgg tgaagtcgat tgattgatga aatataccaa atcgccttcg
    12961 catgctggtg tactggtaaa agctgcaaca ggagaaggca atacctctac aaacaaggtc
    13021 agagaatcgg tacatccaat actgttttca acggtaatag taaccggtat ttcaccggaa
    13081 tcaacaaatt cgtgtaacac ctgcgtccct tcaatattat atgtgccatc gaaattccac
    13141 cataccgaag taattacacc ctcggaagag atgctattgt cggtgaaaac aatcaggttt
    13201 ccgctgcaag caatatctgg cgaaaaatcc ggaactggca gaccatgaat tagcaatgct
    13261 tcattgaatg aatttgaaca cccgtcgctg ttttcaacaa taagcccaat aagatagtta
    13321 cctgctgaat caaagacgtg agtaaagtct tgattatttg agacaatttc tccattaata
    13381 ctccattccc attg
//
LOCUS       DAOOQP010000083        13130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_5835_length_13130_cov_6.405660, whole genome shotgun sequence.
ACCESSION   DAOOQP010000083 DAOOQP010000000
VERSION     DAOOQP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_13130_cov_6.405660"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2790)
                     /locus_tag="PLK75_09040"
     CDS             complement(<1..2790)
                     /locus_tag="PLK75_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY39430.1"
                     /translation="MSIYSSAVKKPITTIMIFLAVIIFGLYSLTRLPIDLYPEVELPA
                     ISVVTIYAGANASDIETNVSKPIEDACNSVENLKEINSTSRDNMSIVTLEFEWETNLD
                     EAANNIRNSLEFVKKNLPDGAETPIIYKFNSSMMPLLFYSVVAKESYPGIEKLLDEKL
                     VNRLNRIDGVGSVFIAGAPGRAVYVEVDPRKIEAYNMSVEMIGGILQAENFNLPAGNV
                     EMGQMDYQLKVEGEFANSDQVKEVVLGSFNGQTIYLKDVATVRDTVKDMSLDERINGV
                     QGIRMFIMKQSGGNTIAVARDVKKELEEIKKTLPPDVKIEPIFDSSEFITHSVNNLSE
                     TLLYALLFVVLVVLFFLGRWRATFIIVLTIPISLIVAFIYLSVTGSSINIISLASLSI
                     AIGMVVDDAIVVLENITKHIERGATPREAALYATNEVWLAVIVTTLVVLAVFLPLTFV
                     SGMTGVLFRQLGWIVSIVVTTSTLAAITLTPMLSSLMLRLRPQKKYPKFGYTNTIRRF
                     LDVVDRAYEKLIRWSLHHKIIVLGSALVIFISSFALMPYIGTEFIPESDQSMVTAKIE
                     LQTGTRVEESAKVVKQVEDIIKKRYGSEVELVSTSAGSNDARGVSAIWSTSGSHIINI
                     QLRLVDIDKRNRTCWEIGDDLRAQLAMIPEIVNYSLAFSSGMGGFGSQDIEVEIFGYD
                     FDVTNKIASELSENIKKLEGARDITISREKSKPELTVVLDREKLAQYGLNTASVSMAV
                     RNRVLGYTATKFREQGDEYDVIVRLKEDYRGSISDIENITLMNAQGVKVRLSEVGKVV
                     EQWSPPTIARRQKERIVSVTVQPYKVSMGELAAQIQKIVDNTDKPGDVMITVGGSYEE
                     QQDSFADLGLLLVISLLLVFIVMASQFESFKMPLIIMFSIPFSFSGVILALFITGTSL
                     SVIAALGAVLLIGIVV"
     gene            complement(2822..3886)
                     /locus_tag="PLK75_09045"
     CDS             complement(2822..3886)
                     /locus_tag="PLK75_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY39431.1"
                     /translation="MNTTKSFLLAGAAVVLLLMSSCGSKKGSDSNAQTAADSVKQIKT
                     VKLLTLSKTEVMRNLEYTASISPYEELYMAPAQPGRITKINVEIGDRVSKGQIIAEMD
                     KTTLNQAELQLINLEKDYKRLDTLMQVGGVAAQQYDQIKTQLNVTRNNVEFLRDNVVL
                     KSPFTGIITAKYFENGELYSGAPNTQVGKAALVIIQQINPLKATIAISEKYYPIVKTG
                     MEAVLTCDIYPGEIFKGKISLIHPTINAMSRTFNVEVQIPNGNEKLRPGMFAKVNVSV
                     GKEEAMVVPVTSVLVQEGTNIRYVFVYIDGKAKRVNVETGKRFDDKIEIFSDNLKEGD
                     KIIVAGQNKLLEGDVVTVVE"
     gene            complement(3910..5295)
                     /locus_tag="PLK75_09050"
     CDS             complement(3910..5295)
                     /locus_tag="PLK75_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY39432.1"
                     /translation="MKRRIIFTVLLLGWLTIGGFSQTSTTLSLSLDQAREYALEHNRV
                     LKNAQYDVAISKKQIWANIAQGLPQAEGSIDYTDFFNYEVELNFGGGGGTPDIDFTKL
                     DAGDLEILSLLNQMMGSGTSTIKMDNSSSAKFQVSQLVFSGQYLVGLKIAKIAHHLTE
                     LALESTEADIIEAVTSSYYIALVTIESSKTLDASIENLNKTLAQTKVMLAAGMAEQVD
                     VDQLELAVMMLQNTKSQMQRAVEMSYNLIRFQLGVDANTEIVLTESLDDIIGQINFQA
                     LAGDSFVLENNPTYKMLLTQEEMTGKMMQLEKWNYAPTIVGFYSYNEKLITTDFDMNP
                     KHIAGVTMTIPIFSSGMRNAKVQQKKIEFEKSKNTRLMVQDQLLMQEKQYRFDLINAI
                     EQYELQKRGIDVAKRIYDNTEVKYTHGVASSLQLTQASDNYLKAQNNYISALMNLLQA
                     KNNFDKLLNNL"
     gene            complement(5397..6008)
                     /locus_tag="PLK75_09055"
     CDS             complement(5397..6008)
                     /locus_tag="PLK75_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY39433.1"
                     /translation="MDTRLRILKEAGILFSRMGIRAVTMDYIACQLGVSKRTIYELFS
                     DKDDLLFQAISEGMNYHKEQMIAMIESSENVIDAIFKFAVFHNRIIEKIHPMFLEDLK
                     KYHSNVYNSLNNNRVFDDDTILEHMLNRGIIDGMFRSDISVKLAGVFMRQTVKFCSLQ
                     HNDGENQFTKEDISNTIFLPYLRGICTEEGLTILNNFKADALY"
     gene            complement(6134..7438)
                     /locus_tag="PLK75_09060"
     CDS             complement(6134..7438)
                     /locus_tag="PLK75_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOY39434.1"
                     /translation="MRIKALFIGGAMFLLLGFTGLASGVARELPQYLLAIPEVQDIDT
                     LETSDFFSEKYLVLFKQEIDHGNPDAGYFTQRVFISHYGFNSPVVYTTEGYTGSYAEM
                     PGYVNELSAMFEANQIVVEHRYFDQSVPEPFDWKYLTTANAAADHHAVFEALKPVYKE
                     KWISTGISKGGETAMLYNMYYPDDVDICVAYVGPVAFAVEDGRHEPFIASVSTGKDRK
                     RILSFQKEVLKRRSEMIPLLEAFSKKNKLTYRVSIDEVLDYCVLEFSFAFWQWGASPA
                     TIPGTKASTQELFRYLVMIASPDYFAIEGIEPTLPFFYQAARELGYYGYDVKPFKKYL
                     SISSAEGYLYTIFLPPDLKVDFIPETSQKLADYMQHKAHHTILIYGEFDPWSAAAVDV
                     SQNPNVLKVISPAGSHMARIKNLPKIQHDKVINTITQWLNEK"
     gene            7661..10048
                     /locus_tag="PLK75_09065"
     CDS             7661..10048
                     /locus_tag="PLK75_09065"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOY39435.1"
                     /translation="MLCTLNAFSQKRVVTAVRTSEKIIIDGSLQEGIWQSAPIQTNFL
                     TYQPTNGQKSQLESDVMVAYNDRAIYVAAFLKDHTPDSIYNEPTIRDDDNGNADMFGI
                     DLNPNNDGQNSYMLWITAANVQTDIRVSTFDSDYSWDAVWESGVQLCDSGWMVEVRIP
                     WSSIRFPNNASQIWGINFYRSIRRTREVSSYNLVDKKSGTKTEQMAEMHGISNIEAPL
                     RLSFTPYLAAYLNHYSELSKPAYAFNGGLDMKLGLSESYTLDMTLIPDFGQEKSDNIV
                     LNLSPYETYYEERRPFFTEGTELFNKCGLFYSRRIGSTPDGFNSVDTLLDDGYTILKN
                     PNQSRLINAFKITGRDKNNLAVGFFNAITANTYARVTNPLGESEKLLTEHWANYNMIV
                     VDKAFNKNSYVNLTNANVVQPGNGFLSNVLGTSFRLMDKQNKFGIKTNAAWSYYKRGN
                     TNQQPGGFNTSFGLGKFNGSFNYGYSVNVESDRYNPNAMGFLQQNNEFSQTLSFAYNI
                     FEPKGNFNTILCEVYSTYSRLYSPNTFTGWYLHFWNYALTKKQLSIWNNLHFDLTKTN
                     DYFEPRVSGRFVKWPQMFYDNIYLSTDYRKRLAIDFVANLTVNTSDYRGFTVEISPRL
                     RLNKHIILIVSFNADNDWNDYGYVENSEDNVFFGKRNIGIYTSSLGLNWVFTNKIYLQ
                     LSARHYWSKVDYRSFYLLNEDGSLAPVEITFTDKNINFNAFNVDLLFSWNFAPGSFLN
                     FSYKNNIYNMEYFDTGNIFPRFGENFTNTLQQPQWNTISLKLMYYIDWQYFKKSH"
     gene            complement(10241..11674)
                     /locus_tag="PLK75_09070"
     CDS             complement(10241..11674)
                     /locus_tag="PLK75_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOY39436.1"
                     /translation="MRKEKDFLGEMQIPANALYGIHAARAVANFKNDSTFPVEWYKSV
                     GAVKLACYKTCEKFFELSLEKFGKNLPLTIIESNVLDALKVAATEVSLGKWFEYFIVP
                     AVQGGAGTSINMNINEIIANRALQITGHNPGEYQFIHPVEHANIYQSTNDVIPTAMRL
                     AVIQLLTELEEEVNKLRLLMEETEQRFRNIPRIAYTQMQQAVPTTYGKLFSTYCDALA
                     RDWWRISKCFERIKTVNLGGSAVGTSITVPRYFVMEAVRTLQKNTGLPLTRGENLCDA
                     TANNDALVEVHAILKSHAVNIEKFASDLRLLAADISNENLSIPQKQVGSSIMPGKINP
                     VIPEFLISIAHRVYANDQMIASLCAQGCLELNAYLPSIGNAMIESLKLLIAADISFRE
                     NLLTGINADAGKSEEIYLKSPALSTALLPYIGYEKASEVASYMQQHRCTIFEANKALG
                     FIEEQKLHEALHPDKLLKQGFLIKDLL"
     gene            complement(11679..12842)
                     /locus_tag="PLK75_09075"
     CDS             complement(11679..12842)
                     /locus_tag="PLK75_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOY39437.1"
                     /translation="MARIIVIDDERSIRNTMKDILEYEKHEVDVACDGKEAIGLISEK
                     AYDVAFCDIKMPQMDGLEVLEQIKTINPDLSVVMLSGHGTIETAVESIKKGAFDFIQK
                     PPDINRIMITIRNAMDKSQLVNETRQLKKKVGMKYQMVGSSESINKVKIMIDKVAPTD
                     ARVMIMGPNGSGKEVVARWMHEKSHRSNGPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRIGKFEQATGGTLFLDEIGDMSLSAQAKVLRALQENKITRVGSDKDIAVDVRVI
                     TATNKDLRAEIDNKNFREDLYHRLSVIIIKVPSLNERIDDVPELASYFIEMACSEQGI
                     AVKSITPDAIEALQKCKWTGNIREFRNVIERLVILCGNSITASDVDAYVYPLT"
BASE COUNT         3602 a         2927 c         2665 g         3936 t
ORIGIN      
        1 caccacaata ccgatgagca atactgcgcc cagtgcggca ataacgctca agcttgttcc
       61 cgtgataaac agggcgagaa taacacccga gaacgagaaa ggaattgaaa acataataat
      121 caatggcatt ttaaatgatt cgaactgcga agccattaca ataaatacca gtaagagcga
      181 aatcaccaac agcaaaccga ggtcggcaaa cgagtcctgt tgttcttcgt atgacccgcc
      241 cacggtaatc atcacatcgc cgggtttatc ggtattatca acaattttct gaatttgtgc
      301 agccagttca cccatcgaaa ccttataggg ttgtacggtt accgaaacta ttctttcctt
      361 ctggcggcgg gcaatggtag gaggcgacca ttgttccacc accttgccca cttcggaaag
      421 acgcacttta acaccttgtg cattcatgag tgtaatgttt tcgatatcgc tgattgaacc
      481 ccggtaatct tccttgagac gaacgatcac atcgtactca tcgccttgtt cccggaattt
      541 tgtggcggtg tatccaagca cccggttacg cactgccatc gaaacacttg ctgtattgag
      601 tccgtattgt gccagttttt cacgatcgag cactacggta agttcgggtt ttgatttttc
      661 gcggctgatg gttatatcgc gtgcaccttc gagctttttg atattctccg acagttcaga
      721 cgcaattttg ttggttacat caaagtcgta accaaaaatt tcgacctcaa tatcctggct
      781 gccaaaccca cccataccac tcgaaaatgc caggctgtaa ttcacaattt caggaatcat
      841 ggcgagttgg gcacgcaggt cgtctccaat ttcccagcat gtacgattac gtttatcgat
      901 atcgaccagg cgaagttgta tgtttataat gtgggatccg gaagtactcc agatagccga
      961 aacacctctg gcatcgttac tacctgctga tgttgaaacc aactcaactt cgcttccata
     1021 ccgtttttta attatatctt cgacctgttt caccaccttg gccgattctt cgacacgggt
     1081 tcctgtctga agttcaattt ttgcagttac catgctttgg tctgattccg ggataaactc
     1141 agtgccaata tatggcatca gggcaaacga agaaataaaa atcaccagtg ctgatcccag
     1201 tacaataatt ttatggtgca agctccagcg tataagtttt tcatacgccc tgtcaacaac
     1261 atcaaggaag cgcctgatgg tattggtata accaaatttg gggtattttt tctgtggacg
     1321 caggcgcagc atcagcgagc tgagcatagg ggtaagggta atggctgcca gtgtagaggt
     1381 tgttactact atcgaaacaa tccaacccag ctgacggaac aacacaccgg tcataccact
     1441 tacaaacgtc aatggcagga atactgcaag taccaccagg gttgttacga taaccgccag
     1501 ccacacttcg ttggtggcat acagggctgc ttcgcgcggt gtagctcccc tttcgatgtg
     1561 ttttgtaata ttttcgagta ccacaatggc gtcgtccacc accatcccaa tggcgattga
     1621 aagcgaggcc aacgaaataa tatttatgga actccccgtt accgacaggt agatgaatgc
     1681 tacaatcagc gatataggaa tggtgagaac aataataaat gtagccctcc agcgtccgag
     1741 gaaaaacagc acgacgagca ccacaaagag caatgcataa agtagtgttt ccgacaggtt
     1801 gtttaccgaa tgggtaataa attctgacga gtcgaatatg ggttcgattt ttacatcagg
     1861 cggcagggtt ttctttattt cctcaagctc tttctttaca tcgcgggcaa ccgcaatggt
     1921 gttacctccc gattgcttca tgataaacat tctgataccc tgcaccccat taattctttc
     1981 gtcgagcgac atatccttca ccgtatcgcg caccgtggcc acgtctttca ggtagatggt
     2041 ctgtccgtta aaactgccca acaccacttc cttcacctga tcgctgttgg cgaattcgcc
     2101 ttccacttta agctgataat ccatctgacc catttcgaca ttaccggcag gcaggttaaa
     2161 gttttccgcc tgaaggatac cgccaatcat ttcgaccgac atgttgtagg cttcaatttt
     2221 acggggatca acttcgacat acaccgccct tccgggagca ccggcaataa aaaccgagcc
     2281 gactccgtcg atacggttga ggcggttaac cagtttttca tcgagcagtt tttcgatacc
     2341 cggatagctt tctttggcga ccaccgaata gaacagtaag ggcatcatac tgctgttgaa
     2401 tttgtaaatt atgggtgttt cggccccatc gggtaagttt ttcttcacaa attcgagtga
     2461 gtttctgata ttgttggccg cttcatcaag gttggtttcc cattcgaatt ccagggtaac
     2521 aatcgacata ttgtcgcgtg aagtggagtt tatttccttc agattttcga ccgagttgca
     2581 agcatcttcg atgggtttcg acacgtttgt ttcgatatcg gaggcattgg ctcccgcgta
     2641 gattgtaact accgaaattg ccggaagctc tacttcggga tataaatcga tgggcagacg
     2701 ggtaagcgaa tacaacccga aaatgattac agccaaaaat atcattatgg ttgtaatcgg
     2761 tttttttact gcactactat aaatactcat aattcctgtt ttttaaaaaa ttttatccct
     2821 cctattcgac cacggttacc acatcgcctt cgagcaattt attctgtccg gcaacgataa
     2881 ttttatcgcc ttccttcagg ttgtcagaaa aaatctcgat tttatcgtcg aatcgttttc
     2941 cggtttcaac attcacccgt ttggccttac catcgatgta aacaaaaaca tagcggatgt
     3001 tggtaccttc ctgaacgagc accgaagtta ccggcaccac catggcctct tctttaccaa
     3061 cactaacatt tactttggca aacattccgg ggcgtagttt ttcgtttcca ttcggaatct
     3121 gtacctcaac attgaaagta cggctcattg cgttgatggt gggatgtatc agactgattt
     3181 tacctttaaa tatttctccg ggatagatgt cacatgtcag cacagcctcc attccggttt
     3241 ttacgatagg ataatatttt tctgatattg caatggtggc cttcaatggg tttatctgct
     3301 gaattatcac caaggctgct ttaccaacct gtgtgttggg tgctccgctg tagagttcgc
     3361 cattttcgaa atatttggcg gtaataattc cggtaaaagg tgacttcaga accacattat
     3421 cgcgtaagaa ctccacattg ttgcgggtta cattcaattg ggtctttatc tggtcgtatt
     3481 gctgagcggc aacaccacct acctgcatga gagtatcgag gcgcttataa tctttttcga
     3541 gattaatcaa ctgcaattcg gcctgattca gggtagtttt gtccatttcg gcaattatct
     3601 ggcccttact cacgcggtcg ccaatttcga catttatttt agtgattcta cccggttggg
     3661 cgggcgccat atacaattct tcgtagggtg aaatactcgc tgtatactca agatttcgca
     3721 taacctcggt ttttgaaagg gtcaacagtt ttaccgtttt aatctgcttc accgagtcgg
     3781 cagctgtttg ggcattacta tcagaaccct ttttagatcc gcacgacgac atcaacagca
     3841 atactaccgc tgctcctgcc aataaaaatg atttagttgt attcattgct tgttcgctat
     3901 ttaaaaaatt tacaaattgt ttaacagttt atcgaaattg tttttggcct gaagcaggtt
     3961 catcagcgcc gagatgtaat tattctgtgc tttaaggtag ttgtcgctgg cctgagtaag
     4021 ctgcaaacta ctggcaacac catgggtata ttttacttcg gtattgtcgt aaatacgctt
     4081 tgccacatcg ataccacgct tttgtaattc atattgttct atggcattaa tcaaatcgaa
     4141 acggtattgc ttttcctgca tcagcaattg atcctgcacc atgaggcgtg tgttttttga
     4201 tttttcgaat tcaattttct tttgctgtac tttggcattc cgcataccgc tcgagaaaat
     4261 cgggatggtc atggtaacac cggcaatatg tttggggttc atatcgaaat cggtagtaat
     4321 caatttttcg ttgtagctgt agaaacctac aatggttggt gcatagttcc atttttcgag
     4381 ctgcatcatt ttcccggtca tttcttcctg agtcagcagc attttgtaag taggattatt
     4441 ttcgagcaca aaggaatcgc cggccaatgc ctgaaagttg atttgaccaa tgatgtcgtc
     4501 gagtgattcg gtaagaacta tttcggtatt tgcatcaaca ccgagttgaa aacgaatcag
     4561 gttataactc atctcaacag cgcgctgcat ctgacttttt gtattctgaa gcatcattac
     4621 cgccaattcc aactgatcca catctacctg ttcggccata cctgctgcca gcattacctt
     4681 ggtttgggcc aaagttttat tcaggttttc gatactggca tcgagtgttt tagacgactc
     4741 gatggtaacc agcgcaatat aatatgatga tgtaaccgct tcgataatat cagcctcggt
     4801 actttcgagg gcaagttctg tcaggtgatg tgctattttg gcaatcttaa gtcccaccag
     4861 atactgcccc gaaaaaacga gctgagaaac ctgaaatttg gccgaagacg agttgtccat
     4921 tttgattgta ctcgtaccgc ttcccatcat ttgattcaac aaactcagta tttcgaggtc
     4981 gccggcgtcg agctttgtaa agtcgatatc gggagttccg cctccgccac caaaattgag
     5041 ttccacttcg taattgaaaa aatcggtata atcaatgctc ccttcggctt gaggcaagcc
     5101 ctgcgcaata tttgcccaaa tctgcttctt cgaaatggcg acatcgtatt gtgcattttt
     5161 aagtacacgg ttgtgctcga gtgcatattc tctggcctgg tcaaggctga gagagagggt
     5221 tgtgctggtt tgcgaaaatc cacctattgt taaccaccct aacaatagta ccgtaaatat
     5281 tattcttctt ttcatcttaa atcttttttc aaaaaacaac aaaaatatgt ctgcaatata
     5341 tcaatcactt cgatattcgg taaactgcct caaggcagcg gtaaattacg attccatcaa
     5401 tacaaggcat cggctttaaa attatttagt attgtcaaac cttcttcagt acaaatcccc
     5461 cgtaaatagg gaagaaatat tgtgttactg atatcttcct ttgtaaactg attttctcca
     5521 tcgttatgtt gcaaagagca aaactttacg gtctgccgca taaaaactcc ggcaagtttc
     5581 acacttatat cagaacggaa cattccgtca ataatccccc tattcaacat atgctccaaa
     5641 atagtgtcat cgtcaaatac tctgttgtta ttcagtgaat tataaacgtt gctgtgatac
     5701 ttttttaaat cctccagaaa catcgggtga atcttctcga ttattctatt atgaaatact
     5761 gcaaatttaa atattgcatc tattacgttt tccgatgatt caatcatagc aatcatctgc
     5821 tctttatgat aattcattcc ttcggaaata gcctgaaaca acaggtcgtc cttatcggaa
     5881 aacaattcat atatcgtccg cttcgaaaca ccgagctgac acgcaatgta gtccatggtt
     5941 acggcacgta ttcccatgcg cgaaaacagg attccggctt ccttcagtat tcttaatcgg
     6001 gtgtccatca caataattaa tcacgcaaaa ctaaaaaact ttttttagtt tcaatagttt
     6061 tattaatcat aaatgacgat tttaacaaat atttaatatt gttgcaatcg attacccctg
     6121 caaacagagg gggctacttc tcgttaagcc attgagtgat tgtattaatc actttatcat
     6181 gttgaatttt tggtaggttt ttgattctgg ccatgtgcga acccgcaggg gaaatcactt
     6241 tcaaaacatt tgggttttgc gacacgtcga ctgctgctgc tgaccaaggg tcaaattcac
     6301 cataaataag tattgtatgg tgagccttgt gttgcatgta atcggcaagt ttttgcgaag
     6361 tttcgggaat aaaatcaacc ttcaaatcag gaggcagaaa tattgtataa agatatcctt
     6421 cagcactact gatgctcagg tattttttaa atggtttcac atcataacca taatacccca
     6481 gttcacgtgc tgcctgatag aagaagggta gagttggttc aattccttca atggcaaaat
     6541 aatcggggga ggcaatcatt accagatacc gaaaaagctc ctgagtggat gcttttgtgc
     6601 cgggtattgt tgccggagat gcgccccatt gccaaaatgc aaacgaaaat tccagtacac
     6661 aataatcgag aacttcatcg atgcttactc tgtaggttag tttgtttttt ttcgaaaagg
     6721 cttcgagcag gggaatcatt tcactcctgc gcttgagcac ttctttctga aaactaagta
     6781 tccgtttacg gtctttcccc gtgctgaccg atgcaataaa cggttcgtgg cgaccatctt
     6841 caaccgcaaa agctacaggt ccaacatatg caacacaaat gtcaacatca tcagggtagt
     6901 acatattata caacatggct gtttcaccac ccttactaat tcctgtgctt atccattttt
     6961 ctttatacac aggctttagt gcttcgaaaa cagcgtggtg atcagcggca gcatttgcgg
     7021 ttgtaaggta tttccaatcg aaaggttccg gcaccgactg atcgaaatac cgatgctcca
     7081 caacaatctg atttgcctcg aacattgcag aaagttcgtt gacatagccc ggcatttctg
     7141 catacgatcc ggtatatcct tccgtggtat aaacaacagg actattaaaa ccataatgac
     7201 ttataaaaac gcgttgagta aagtagccgg catccggatt gccatgatca atttcctgct
     7261 tgaacaatac caggtatttt tcgctaaaga aatcggaagt ttcaagggtg tcgatatcct
     7321 gcacttccgg gatcgcaaga aggtattgcg gtagttcacg tgcaacgccc gaggccagtc
     7381 ctgtaaaacc aagtaacagg aacatagcac caccgataaa caaggcttta attctcataa
     7441 gattagtttt aagcatacca agatagcaat aagttttatt ctttatcaga tggcaaggta
     7501 acaaaaacta cattgaaatt ttaacgaaaa gatcatttta ataattcata aagaattttc
     7561 actgttgggt gtttttgttt ttgatcaaat tgtaccatca ttgtatttta aatatcttcg
     7621 tgaaagaatg aagctgttgc ctgttttttt tatcctctgt atgctgtgca cactgaatgc
     7681 gttttcgcag aagcgcgtgg ttactgccgt tcgtacttcg gaaaaaataa tcatagacgg
     7741 cagtttacag gaaggaatct ggcaatcggc acccatacaa acgaattttt taacgtatca
     7801 gcctacaaat ggacagaaat ctcagttaga aagtgatgta atggtggctt ataatgacag
     7861 agcgatatac gttgcagcct ttctgaaaga ccatactccc gacagtattt acaacgagcc
     7921 aaccatccgc gacgatgaca acgggaatgc cgatatgttt ggtattgacc taaacccgaa
     7981 caacgacggg caaaactcat acatgttatg gataactgct gccaacgtac agaccgatat
     8041 tcgtgtttct accttcgaca gtgattattc gtgggatgca gtttgggaaa gcggtgttca
     8101 gttgtgtgac tcggggtgga tggtcgaagt gcgtatccct tggtcatcga ttcggtttcc
     8161 gaacaatgcg tcgcagatat ggggaatcaa tttctacaga tcaattagaa gaacgcgcga
     8221 agtttcaagc tataatctgg tagataaaaa atctggaact aaaacagagc aaatggcgga
     8281 aatgcatggt atatcaaaca ttgaagcccc tttacgcctg tcgtttactc cctatttggc
     8341 agcctattta aaccattatt ccgagttgtc caaaccggcg tatgcgttca atggcggtct
     8401 tgacatgaaa cttgggctta gcgaaagcta tacactcgat atgacattga ttcccgactt
     8461 cgggcaagag aaatccgata atattgtact gaatctgtcg ccctatgaaa cctattacga
     8521 agaaaggcga ccgtttttta cggaaggcac cgaattgttc aacaagtgtg ggctgtttta
     8581 ttcgcgccga atcgggagca cccccgacgg gtttaatagt gttgataccc tgctcgacga
     8641 tggctacaca atattgaaaa atccaaatca atcgaggctg attaatgctt tcaaaattac
     8701 cggccgcgat aaaaacaatc ttgccgtcgg ttttttcaat gccatcaccg ccaacaccta
     8761 tgctcgtgta accaaccccc ttggcgaaag cgaaaaatta cttacggagc attgggcaaa
     8821 ttataacatg attgtggttg ataaagcatt caataaaaac tcatacgtaa accttaccaa
     8881 tgccaacgta gttcagccgg gcaacggatt cctttccaac gttctcggaa cttcgttccg
     8941 gttaatggat aagcaaaata aattcggcat aaaaaccaat gccgcttgga gctattataa
     9001 gcgtggtaat accaatcaac agcccggtgg ttttaatacc tctttcgggc tagggaaatt
     9061 caatggttcg tttaattatg gatacagcgt aaatgttgaa tcggaccgat acaatccgaa
     9121 tgctatggga tttcttcaac aaaacaacga attcagtcag accttgagtt ttgcctataa
     9181 catttttgag ccgaaaggaa atttcaacac cattctttgc gaggtttatt ccacatattc
     9241 gcgactatac agtccaaaca catttacagg ctggtacttg catttctgga attatgccct
     9301 gacgaaaaag cagttgagta tatggaataa tcttcatttt gacctgacaa aaacgaatga
     9361 ttattttgaa cccagagttt cgggtcgctt tgtaaaatgg cctcaaatgt tttacgataa
     9421 catatacctt tcaaccgatt accgcaaacg actggccatt gatttcgttg ccaatctaac
     9481 cgtgaacaca tccgactatc ggggatttac cgttgaaatc agcccccggc ttagacttaa
     9541 taaacatatt attttaattg tatcgttcaa cgccgacaac gattggaacg actatggcta
     9601 tgtagaaaac tcggaagata atgtgttttt cggaaaacgg aatataggca tttataccag
     9661 cagtttgggg ttgaactggg tgtttacaaa taaaatatat ctgcagctaa gtgcacggca
     9721 ttactggagc aaagttgatt atagaagttt ttacctgttg aatgaagacg gaagccttgc
     9781 tcctgttgaa attacattta ccgataaaaa tatcaatttc aatgcgttta acgtggattt
     9841 gttgttcagt tggaattttg ctcccggaag tttcctgaat ttctcctata aaaacaacat
     9901 ttacaatatg gagtatttcg atacgggaaa tatctttccg cgatttggtg aaaattttac
     9961 aaacacgtta cagcaaccac agtggaatac catatcactc aagcttatgt actatatcga
    10021 ctggcagtat tttaaaaaat cgcactaaaa tttttccgcg gacaaaagaa aacaaaaaaa
    10081 ggcagcacaa tcaaacaacc cttgcgattt acaatatatt gccatggcag aaaaaggcaa
    10141 tctcatcatc cgaatgaaaa cattgggact tatgagtatc gatcgtaaaa ccggcaacgt
    10201 gaactggact ttttcgttta aatagctgaa ttgttcacac ttacaataga tctttaatca
    10261 aaaacccctg ttttagcagt ttatcggggt gcaaagcttc atgcagtttt tgttcttcga
    10321 tgaagccaag tgctttgtta gcttcaaaaa tagtgcagcg gtgttgctgc atatatgacg
    10381 caacttcgga agctttttcg taaccaatat aaggaagcaa tgcggtgctg agggcagggc
    10441 ttttcagata gatttcttcg ctctttcctg catctgcatt aattcccgtc agtaagtttt
    10501 cgcgaaaact gatatcggct gctataagga gcttcaggct ttcgatcatg gcattcccga
    10561 tggaaggcag gtatgcattc agttctaggc aaccctgtgc acaaagcgaa gcgatcatct
    10621 ggtcgttagc atacacacgg tgtgcaatgc taataagaaa ttccggtatt accggattga
    10681 ttttaccggg cataatgctg gagcctacct gcttttgcgg tattgacagg ttttcattgc
    10741 tgatatctgc agcaagcaac cgtaagtcgg aggcaaattt ttcaatattt accgcatgcg
    10801 atttcaggat ggcatgcact tcgaccagtg catcgttatt tgcggtagca tcgcaaaggt
    10861 tttcaccacg ggttagcggt aatcctgtgt ttttctgcag ggttcttaca gcttccataa
    10921 caaaatatct gggcactgtt atgcttgttc ctacagcgct tccaccaagg ttgaccgttt
    10981 tgatgcgttc aaaacatttg gaaatacgcc accagtcgcg cgcaagggca tcgcagtacg
    11041 tactaaagag ttttccgtaa gtagttggaa cagcctgttg catttgggta taggctatcc
    11101 gaggaatatt ccgaaatcgc tgttcagttt cttccatgag cagacggagc ttattcacct
    11161 cttcttcgag ctcggtgagc aactgtatta ctgcaagccg catcgctgtt ggaattacat
    11221 cgtttgtgct ttggtaaatg ttggcatgct ccaccgggtg aataaattga tattctcccg
    11281 ggttatgtcc tgtaatctgc agtgcacgat ttgcaattat ctcattgata ttcatattaa
    11341 tactggtacc cgctccaccc tgaactgcag gaactatgaa atattcgaac catttgccca
    11401 ggctcacttc ggtagcagca actttgagtg catcaagaac attactttca ataattgtca
    11461 gcggcaggtt tttcccaaat ttttcgaggc tcaattcaaa aaacttttca catgtcttat
    11521 aacaggcaag ctttacagcc ccaaccgact tgtaccattc cactggaaag gtactatcat
    11581 ttttaaaatt agcaacagcc ctagctgcgt gtattccata cagggcattt gcaggaatct
    11641 gcatttcgcc taaaaagtct ttttcttttc tcataagtct aggttaatgg atacacatag
    11701 gcatccacgt cgctggcagt tattgagttt ccacaaagta taaccaacct ctctatcaca
    11761 ttacggaatt cacggatatt tcctgtccac ttacattttt gcaatgcctc aatggcatca
    11821 ggagtaatgc ttttcaccgc aattccctgt tctgagcagg ccatttcaat aaaataggat
    11881 gccagttcag ggacgtcgtc aattcgctcg ttgagcgacg gaactttgat gataatgacg
    11941 ctcagacggt gatagagatc ttccctgaaa tttttattat cgatttcagc tcgtaaatct
    12001 ttgtttgtag cagtaataac ccttacatcg acagcaatgt ccttatcgct gcctacacgg
    12061 gtaattttgt tttcctgcag ggctctcagc acttttgcct gagccgacag actcatatca
    12121 ccaatttcgt cgagaaacaa ggtacccccg gttgcctgtt caaatttacc aatacgctgc
    12181 tttattgctg aagtaaaagc acctttttcg tgcccgaaca gctcgctttc aattagttct
    12241 gaaggtatgg cagcgcaatt cacttccaca aaaggaccgt tacttcggtg acttttttcg
    12301 tgcatccatc gtgctaccac ctctttccca cttccattgg gtcccattat catcacccgt
    12361 gcatcggtgg gggcaacctt atcgatcatg attttaactt tattgataga ctcgctgctg
    12421 cctaccatct ggtatttcat tccgaccttc ttctttaact gccgggtttc gttcaccaac
    12481 tgtgatttgt ccatggcgtt gcggatagtg atcatgatac ggttgatatc cggcggtttc
    12541 tgaataaaat cgaaggctcc ctttttaatc gattctactg cagtttcgat ggtgccatgt
    12601 cccgaaagca taaccaccga taagtcgggg ttgatggttt taatctgttc gagaacttcc
    12661 agtccgtcca tctgaggcat ttttatatcg caaaaagcca catcgtacgc tttctcgcta
    12721 atcaacccga ttgcctcttt tccgtcgcag gcaacatcaa cttcgtgttt ctcgtattcg
    12781 agaatgtctt tcatagtgtt gcgtatgctg cgctcatcgt cgataactat aattcgtgcc
    12841 atatatcaaa ctgttttcgg taaaattaat aaagaaatcg ttgcagaaaa gcaattattt
    12901 tagaatgcaa catataccaa tgcatttgta cgttgtttct cagtaaaaca atgtgtcgtt
    12961 tgttgtgctt cgaatcccct gtctatgctt caattatcgt ttcgcttcgc ttctctactc
    13021 agcatgacag gggcataaaa aaaaagctgc cctttcggac agctttttaa catgtaagtt
    13081 tttttaatat ttgtatttga atacagcgat attacctgtt gtgaaatcgc
//
LOCUS       DAOOQP010000084        12371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6322_length_12371_cov_5.577460, whole genome shotgun sequence.
ACCESSION   DAOOQP010000084 DAOOQP010000000
VERSION     DAOOQP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6322_length_12371_cov_5.577460"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..578
                     /locus_tag="PLK75_09080"
     CDS             282..578
                     /locus_tag="PLK75_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39438.1"
                     /translation="MNSEHETLLLNLQDKVEKLSALFFVEKEKNNKLLSELEQYKLSL
                     SDSKQQIETLKSKYENLRFAGAFRANSEIEQAEAKQRLNRIIREIDQCIALLNK"
     gene            580..894
                     /locus_tag="PLK75_09085"
     CDS             580..894
                     /locus_tag="PLK75_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOY39439.1"
                     /translation="MDEKLTIHVTISERTYPINIKRNDADGEARIRQAADLINNTVSQ
                     FRRMGYVNRDEQDYLAMSALQICLKLIEKDNNVDIAPIINKLKDISLIIDEILEKEQK
                     VL"
     gene            1133..2671
                     /gene="rny"
                     /locus_tag="PLK75_09090"
     CDS             1133..2671
                     /gene="rny"
                     /locus_tag="PLK75_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOY39440.1"
                     /translation="MEIMIWIVGAAALVAGGGVSYFVWDKALVRKKDRMIRDAEQESE
                     VIKKEKILQAKEKFLQLKAEHETQINERNQKVLVGENRIKQKEGALSQKLEEVQREKK
                     EMEMLRESIDTQKERLARKEEELEKLHKEQVIKLETLSGLSAEEAKKQIMESLKNEAR
                     TESMALVNEIVEEAKMTANKKAKKIVVETIQRVASEVTIENAVTVFHIESDELKGRII
                     GREGRNIRALEAATGIEIIVDDTPEAIILSGFDPVKREVARLALHQLVTDGRIHPARI
                     EEVVNKVAQQVEEEMIETGKRTTIDMGVHGLHPELIRLIGKMKYRSSYGQNLLQHSRE
                     VANLSGIMAAELGLNAKLAKRAGLLHDIGKIPDEETDLPHAIYGMRLAEKCKERPEVI
                     NAIGAHHDEVEMTSLISPIVQVCDAISGARPGARREIVESYIKRLKDLEALALSYPGV
                     MKTYAIQAGRELRVIVGSEKVTDADTEKLSQEIAQKIQTEMTYPGQVKITVIRETRAV
                     NYAK"
     gene            2677..3669
                     /locus_tag="PLK75_09095"
     CDS             2677..3669
                     /locus_tag="PLK75_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY39441.1"
                     /translation="MSKPILCGKKVLVTGGAGFIGSNIVDKLLKQGNKVVCLDNFATG
                     KRENLADALNHPNFTLIEGDIRNFEICCTAVEGIEIVLHQAALGSVPRSINDPKTTND
                     VNIGGFMNMIWAAKQAGIKRFVFAASSSTYGDNKDLPKVEDTIGRPLSPYAVTKYVNE
                     LYAKVFSDLFGITTIGLRYFNVFGKRQDPNGSYAAVFPKFISNFLRHEAPLIHGDGTQ
                     SRDFTYVDNVIQANELAAVTENTEAFNTVFNIAYGESNTLNNLTQEIKSLLLNYDAEI
                     NSIEPMFGPERTGDIRHSLASIEKAKRLLGYAPEFNVHQGLKLAIDWYYRQLKA"
     gene            3676..4425
                     /locus_tag="PLK75_09100"
     CDS             3676..4425
                     /locus_tag="PLK75_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HOY39442.1"
                     /translation="MLPKQGIIIQARLGSTRFPGKILRDFSGGRSILQIQCEKLTGLN
                     ETQLIVATTTSEKDDDIEMFCKDHDLLCFRGSENDVLKRFIDCAHKYEFTHVIRVCSD
                     NPFLSGKGVAQLIQLQANSPDSDYIAFKIGDKPSILTHFGLWAEAVRVSALKRIANTT
                     NESFYHEHVTNYIYNNPDLFNITFIDLGSFWESRQQLRFTVDTPEDFMNMAVLYALTS
                     NNDDAEQLVYAAEQSPALLESMKKQIIKNTK"
     gene            4425..5465
                     /locus_tag="PLK75_09105"
     CDS             4425..5465
                     /locus_tag="PLK75_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOY39443.1"
                     /translation="MKPVYVIGEIGQNHNGSVDIAKQLIDIVAVPVVDELFNRELKPM
                     NAVKLTKRDLNEELSASAMMRPYDSPNSFGKTYGEHRKALELDDEQHAELYRYAKSKG
                     LDVVETLCAKGCLVMLKHFLPDRLKVASRDLTNLPLLAAMAETKIPMILSTGMGGVNE
                     LENALQVITKFHSDISILHCLSEYPSDYKNVNLRTINYLQKHYPQNTIGYSDHTLGIM
                     APIAAVALGAEIIEKHITLDRNMKGTDHKGSLGPEGIWRMMRDIRNLELSLGVEEFYI
                     SEASKASSVKLERSIATIRNMRKGETITENDLHLLSPGDGYKWAQVGLIIGKKLIADV
                     AENEIIYPNMIE"
     gene            5467..5970
                     /locus_tag="PLK75_09110"
     CDS             5467..5970
                     /locus_tag="PLK75_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOY39444.1"
                     /translation="MIKLVLTDIDGVWTDGGMYYDQTGNESKKFNTTDSAGVLFLHLL
                     NIPVGIITGEDTEIVKRRAAKLKVDYVFMGVQNKLLVARELCEKLNISLSEVAYIGDD
                     LNDINLLKAAGMSAVPYGAPGYVACHANMQLTKKGGDGVFREFVETLLGPDLLQQAIG
                     LYLDSQR"
     gene            5970..7328
                     /locus_tag="PLK75_09115"
     CDS             5970..7328
                     /locus_tag="PLK75_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39445.1"
                     /translation="MNGSNINQIEVNYPVGEIRANGFAMWPVLRIYLGDKLLFDKDRR
                     VKIAAINKKLFIKSLLFGLFPIFKKADYLVFSVNSQRRILNGFMVDRFDYLTEKLSNC
                     LFAESPSPLHSKRSQIPTKNIMSKSWFHVFEMMISVFFRPRWKGNDILHQILQESGQQ
                     LEYRSIAKRYLAQYKIMKFLIKHKKIKALFLITSYTNMGYVKACRDSSIPVVEMQHGV
                     INASHYAYNVAVDIDAHNYPDYLLTWGNFEKHIFSEKNFFIQQDNVYPVGHFYLDYIN
                     SRNNTDEVLQQKLSGYKLTLAFTAQDAFEEKVLPLIIELAQRKPEWAIVYIPRRKTED
                     QYPTLPCNIIFASHLNCYQIIAQTDWHITVSSTCALEAPAIGKPNVLLNFENRSQLFF
                     GATFSGKTHTRFVRDIDELITSLENDFIPNRSTIIDESNELFAADFKNNVINFISQVF
                     HK"
     gene            7325..8593
                     /locus_tag="PLK75_09120"
     CDS             7325..8593
                     /locus_tag="PLK75_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY39446.1"
                     /translation="MKLGQHKILFKTAGVYTFANFLNAAIPFLLLPFMTTHLSPGDYG
                     IVAMFQVLMQFTTPFVGMNATSALTRQYYERDTINIKQYVSDSFFITMVTGIFITLLF
                     ILFKSPLASITQFPENWLFVVVVYCLAQNIGLIILSLWQVQYKPVPYGIFRIGRTVLD
                     ITFSVCLILFVGMKWEGMVIGQTSATILLSFVALFFLLKGKWLTVKVNKAYVIDALKF
                     GVPLIPHVVGAIIITYSDRLFITNMVNLEETGLYSVGYNVGMIVYLVQNSFNQAWVPW
                     FYEKLKNNLIADKIKIVKFTYAYYVAMAAIVAVITLGAPWFFATFLGKDFHSAVQFVF
                     WIALGFGFNGMYKMVVNYIFYLKKTYIISIMTVVTAVINIVLNYFFIKSMGAIGAAKA
                     TAVSFFIEFIVVWIISSRLYKMPWLLRIKE"
     gene            8590..9738
                     /locus_tag="PLK75_09125"
     CDS             8590..9738
                     /locus_tag="PLK75_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY39447.1"
                     /translation="MIGILSYFPTLAFIPHYAFALTFIYAVIRRKDIVINHFSSLQAI
                     KSNFLLLLLIASLSVANCFIGVVFYGTEPGFPYPVLMLATYFIALSLSKTDLKFIFWL
                     AVIESLVVVAEFASGTSSFIPAYASMDNPDTDLLYFSRPMGLSYNSSIVAYKLLVAIL
                     IAEYLDKRTLLYRLAQVVLLAGIILTFSRTILVVLVIYFAIRYSRQYLIAFVELVRFR
                     IRYTNLVFLFAFSAFIVVSFYFAYTNFSSISTQFTRGRGNIEFSGREKIWPQFIDFIK
                     KNPLAGNHSLKFYADYHGIPDSAHAHNSFLQVLADNGIFIFALYMIFVFANINRKNVY
                     YILPILIYSITQYGVFWGISLLDIVFMMILLRKDKELTMLTCKPIQIS"
     gene            10274..11629
                     /locus_tag="PLK75_09130"
     CDS             10274..11629
                     /locus_tag="PLK75_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39448.1"
                     /translation="MINYSFAQTEWITEIPVFGDACSMLRTKDSCYAVLKCPIYYNAD
                     STNYFYDLQLSKVTPQGELLWYDTVFSDTLDFVDLSFIQLSNEDYFITGQCWCCPFYC
                     IVSKEGELKYFDRLENYMGTYSSFFSPIEISDTEVWVLGGYSQMYITKINLTNHEVSV
                     DSSALCIHNASWPLMESLSDNRILVASNYLTNIYIIDIAQNVINQTSLNSRFFNPRYK
                     PFHINPYDSLYYFYVQTLDPYPNIRYGFMVLNSQLDSIKFIDNTEFYPNQSQVEYPRI
                     LDFDFPKPGNITMTGYIYWYPGYSEELPYIHSYNYIEDTDEYFHAYVGSVTRYSINML
                     KDGNGYVMLHAFQGNPGFVFLVKENTDTLTNRVPDLEYDTAVKIYPNPAGAYITIESN
                     KTTFSEIIILNAVGNTISDITFGETRQKEISVEKLAPGRYFIKIRNNKNQTHITSFVK
                     S"
     gene            11714..12319
                     /locus_tag="PLK75_09135"
     CDS             11714..12319
                     /locus_tag="PLK75_09135"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOY39449.1"
                     /translation="MNNQNTHIASIKLIIRKYFLFFTKFSCVIILVVCNSNCFAQWDD
                     ITPLNGLYNKVLFINQDTGFVIANYGLIIKTYDFGLTWDVVSQNNGYNYKDICFNNNL
                     CFILGSNENLNSQIFIKSADYGQSWDTIVLPNNHGESFLFINDSTALIVGDNILRTTD
                     YCNSFESVMEFSNIGAFYGGLLSIAFVNDSVGFASGIKRAN"
BASE COUNT         3890 a         2324 c         2535 g         3622 t
ORIGIN      
        1 aatctacaaa aaacgaagaa gccagactta tgcgctggct tctttaaaaa gtttttatcg
       61 gacaacagtg attttttaat actatttgaa gtataattga attggtttga tgcataatta
      121 taacgatgca ttgtgtaacc cgcacatagt cgcaatagta cctgaaaaca attagtaaac
      181 cttttcgtat tgaagctttg ttttggttta gtcgtcgaaa aatcaaagct gtacttttaa
      241 aaacaaataa aaatcactat ttttgtatta agatttataa catgaattcc gaacacgaaa
      301 cgttgttgct taatctccag gacaaagttg aaaaactttc agccctgttt tttgttgaaa
      361 aggaaaaaaa caacaaactt ttgtcggaat tagaacaata taaattatct ttgagtgata
      421 gtaaacaaca aatagaaact ttaaaatcaa aatatgaaaa ccttcgtttt gccggagcct
      481 ttagagcaaa cagcgaaata gaacaggcag aagcaaagca aaggttaaac aggataatac
      541 gggaaattga tcaatgtatt gctcttttaa acaaatagaa tggacgagaa actgaccata
      601 catgttacga taagtgagcg cacctatcca atcaacataa aacgcaacga tgctgatgga
      661 gaagccagga tacgtcaggc agcagacctt atcaacaaca cggtaagcca gtttcgcaga
      721 atgggttatg ttaaccgcga tgaacaggat tatttggcaa tgtcggcatt gcagatttgt
      781 ttgaagctga ttgaaaaaga taacaatgta gatattgcac ccataataaa caagctgaag
      841 gatatcagtt taataattga tgaaattctt gagaaggagc aaaaagttct ttaacataaa
      901 aaaattcggc ccgtattaat tctttatagg ttgaaaagct caacactaaa acaattggag
      961 ctatgctctg attatcgacg gcaggcctca ttccattttg gtggaattgc ttaagcacag
     1021 aggaaggcga aaattttcag gatgctccac ccatgttata aacaggggtt tttctaacat
     1081 gtaaagattt taatacgggt tttttaataa cttaaactaa tatattattg aaatggaaat
     1141 aatgatatgg attgttggtg ccgcggcact ggtagcaggg ggaggagttt catattttgt
     1201 atgggacaaa gcgcttgtta gaaaaaaaga caggatgatt cgggatgcag agcaagagtc
     1261 agaggtaatc aaaaaagaaa agatacttca ggcaaaagag aaatttctgc aactcaaggc
     1321 agagcacgaa acacaaatta acgagcgaaa tcagaaagtg cttgtaggcg aaaaccgcat
     1381 taaacagaaa gaaggtgcct tatcgcaaaa gctggaagag gtacagcggg aaaagaaaga
     1441 aatggagatg cttcgtgaaa gcattgacac tcagaaagaa cgactcgcaa gaaaagaaga
     1501 agaactggag aagctgcata aagaacaggt cataaagctc gaaactctat cgggtttatc
     1561 ggctgaagaa gccaaaaaac aaatcatgga atcgcttaaa aacgaagcca gaaccgaatc
     1621 gatggctctt gttaatgaaa ttgtggaaga agcaaaaatg accgccaata aaaaggcaaa
     1681 gaaaatagtt gtggaaacca ttcaacgcgt tgcctctgaa gttacaatcg aaaatgcagt
     1741 tactgttttt catattgaaa gcgacgaact taaaggcaga ataatcggac gggaaggtag
     1801 aaatatcaga gcccttgagg ctgccactgg tatcgaaatt attgttgacg atactccgga
     1861 agctattatt ttgtctggct ttgaccctgt gaaacgcgag gtggcccggt tagcacttca
     1921 tcagcttgtt accgacggac gcatccaccc tgcccgaatc gaggaagtcg ttaataaagt
     1981 tgcacagcag gttgaagaag aaatgattga gacaggaaag cgaaccacca ttgatatggg
     2041 tgtacatggt ttgcatcctg aattgatacg cctgattggt aaaatgaaat accgatcatc
     2101 ctacggtcag aatttgttac aacattcgcg cgaggtggct aacctttctg gaattatggc
     2161 tgccgaacta ggactgaatg caaaattggc taaacgcgct gggctgcttc acgatattgg
     2221 taaaattccc gatgaggaaa ctgaccttcc gcacgctata tatgggatgc gccttgctga
     2281 aaaatgtaaa gaacgccctg aagttatcaa tgccattgga gcccaccacg atgaagttga
     2341 aatgacaagc ctcatttcac ccattgttca ggtttgtgat gccatttcgg gagcacgccc
     2401 cggagcacgc cgcgaaattg ttgaatcgta tataaaacgc cttaaagatc ttgaagctct
     2461 tgcattgtca tatccgggtg ttatgaaaac ctatgcaatt caagccggaa gggagctcag
     2521 ggtaattgtg ggtagcgaga aagttaccga tgccgacacc gaaaaactta gccaggaaat
     2581 tgcccagaag atacagaccg aaatgacata tccgggccag gttaaaatta cagttatcag
     2641 agaaaccaga gctgttaatt acgctaaata aaaaagatga gcaaaccgat tctttgtggt
     2701 aaaaaagttc ttgtaacagg tggtgccggt tttatcggat ccaacatagt agataaactc
     2761 ctgaagcagg gtaataaggt tgtttgcctc gataattttg caaccggcaa gagagaaaac
     2821 cttgccgatg cattgaatca tccaaatttt accttgattg aaggtgatat cagaaacttc
     2881 gaaatttgct gtactgcagt agaaggaatt gaaattgtgc tgcatcaggc ggctcttggg
     2941 tctgtgccaa gatcgataaa tgatccgaaa actaccaatg acgtgaacat tggggggttt
     3001 atgaatatga tttgggcagc aaaacaggcc ggaattaaac gctttgtttt tgcagccagt
     3061 agttcaacgt atggcgataa caaagacctt ccaaaagtgg aagataccat tggacgacca
     3121 ctttctccct atgcggtaac aaaatatgta aacgaattgt atgctaaagt tttttctgat
     3181 ctgtttggga taactactat tggtttacga tattttaatg tatttggtaa acgccaagat
     3241 cctaatggtt cctatgctgc ggtgttcccg aagtttataa gcaattttct caggcatgaa
     3301 gctcccctga ttcatgggga tggcacacaa agccgcgatt ttacgtatgt ggataatgtg
     3361 attcaggcta atgaattggc tgctgttaca gaaaatactg aagccttcaa tacggttttc
     3421 aatattgcgt atggtgaaag caatacgctg aataatttga cgcaagaaat aaaatcgctc
     3481 ttactaaact atgatgctga aataaattca attgaaccga tgtttggccc ggagcgaaca
     3541 ggagatatca gacattccct tgcatcaatt gagaaagcaa aacgattgct gggctatgca
     3601 cccgaattta atgtgcatca ggggcttaaa cttgctatag actggtacta ccgacaattg
     3661 aaagcttaat atacgatgtt gccaaaacaa ggcattatca tacaggcgcg ccttgggtcc
     3721 acaaggttcc ctggaaaaat actgagagat ttttccggtg gcagatcgat tttacagata
     3781 caatgcgaaa aactaacagg attaaatgag acgcaactta ttgtggcaac aactacctct
     3841 gaaaaggatg atgatattga gatgttttgt aaagaccacg acttgttatg ctttaggggt
     3901 tccgaaaatg atgtgctgaa gcggtttatc gattgcgctc acaaatatga atttacccat
     3961 gtaataagag tttgttccga taacccgttt ttgtcgggaa agggtgtcgc ccaactaata
     4021 cagttacaag caaattctcc tgattccgat tatattgctt ttaaaattgg agacaagcca
     4081 agcattctca cccactttgg attatgggct gaagctgtga gggtttctgc tctaaaacgc
     4141 atcgctaata ctacaaatga gtcattctat catgagcatg ttaccaatta tatttacaat
     4201 aaccccgatc tttttaatat tacatttatt gacctcggca gcttttggga gagccgtcaa
     4261 cagttgcggt ttacagtgga tactcccgaa gacttcatga atatggctgt gttatatgct
     4321 ttaactagca ataatgatga tgctgagcaa ttagtgtatg cagcagaaca atcgccagct
     4381 ttgcttgaat caatgaaaaa acagataata aaaaacacca aataatgaaa ccggtttatg
     4441 ttatcggaga aatagggcag aaccacaacg ggtcggtaga tattgcaaaa caacttatcg
     4501 atatcgttgc agttccggtt gtggatgaat tattcaacag agaattgaag ccgatgaatg
     4561 ccgttaagct gacaaaacgc gacctgaatg aagaattgtc ggcttcagca atgatgcgtc
     4621 cttacgattc accaaacagc tttggaaaaa cttatgggga gcatcggaaa gcccttgaac
     4681 ttgacgatga gcagcatgct gaattgtatc gttacgcaaa atcgaaagga cttgatgtgg
     4741 ttgagacctt gtgtgcaaaa gggtgtctgg ttatgctgaa acatttttta cccgacagac
     4801 tgaaagttgc aagccgcgat cttacaaacc ttcccttgct cgctgcaatg gccgaaacta
     4861 aaatccccat gatactttca accggaatgg gaggggtgaa tgagctggaa aatgcccttc
     4921 aggttatcac aaaatttcat tccgatattt ccatactgca ttgtttatcg gaatatccaa
     4981 gcgattacaa aaacgtaaat ttgcgtacca taaattatct gcagaaacac tacccgcaaa
     5041 ataccatagg atacagcgac cataccttgg gtattatggc accgattgca gcagtggcac
     5101 ttggcgctga aattatcgag aaacacatta ccctcgaccg caacatgaaa ggtaccgatc
     5161 ataaaggttc attaggaccc gaagggattt ggagaatgat gcgcgatata aggaatctgg
     5221 aattatcgtt gggcgttgag gaattttata tctctgaagc ttcaaaagct tccagtgtaa
     5281 agcttgagcg gtcaattgca acaatccgca atatgcgaaa gggcgaaacc attaccgaaa
     5341 acgacctgca ccttctgagt cctggcgatg gctataaatg ggctcaggtt gggttgatta
     5401 tagggaaaaa actcattgcg gatgttgcag aaaatgagat catttaccca aatatgattg
     5461 aataatatga taaaactggt gcttaccgac atcgacggag tctggaccga tgggggtatg
     5521 tattacgacc agacaggtaa tgaatcgaag aaattcaata ccaccgatag tgcaggtgtg
     5581 ctttttttgc atttgctgaa cattccggtt ggaataatca ccggagaaga taccgaaata
     5641 gtgaagcgga gagccgcaaa actaaaagtg gattatgttt ttatgggcgt acagaataaa
     5701 cttctggttg cccgtgagct gtgtgaaaaa ctgaacattt cgttaagtga ggtggcatat
     5761 atcggcgacg acctcaacga catcaacctg ctgaaagccg caggaatgag cgctgtaccc
     5821 tatggagccc ccggatatgt tgcctgccat gccaacatgc aactgacaaa aaaagggggt
     5881 gatggtgttt tcagagaatt tgtcgaaaca ctgcttggtc ccgatttatt acaacaggct
     5941 atcggattgt atctcgattc acaacgctga tgaatggcag caacataaat caaattgagg
     6001 taaattaccc tgttggcgaa attcgggcaa acggttttgc catgtggcca gttctcagaa
     6061 tatacctggg tgataaactt cttttcgata aagaccggag ggtaaaaatt gctgcaatca
     6121 ataaaaaact ttttataaaa tcgctgctat ttggattgtt cccgattttt aaaaaagccg
     6181 attacctcgt tttttcggtg aacagtcagc gacgcattct gaatggattc atggtggata
     6241 ggttcgatta tcttaccgaa aagctaagca actgcctttt tgccgaaagc ccaagtcctt
     6301 tgcattcaaa gcgttctcag atacccacca aaaatattat gtcgaaatcg tggtttcatg
     6361 ttttcgaaat gatgatttcg gtttttttcc ggccaaggtg gaaaggtaat gacattttgc
     6421 atcagatact ccaagaatct gggcaacaac tggaataccg gtctattgcc aaaagatatc
     6481 tggcgcagta taaaatcatg aagttcctga ttaaacataa gaaaataaaa gcgctattcc
     6541 ttatcacgtc ctatacaaat atggggtatg taaaagcatg tcgcgactca agcattcctg
     6601 ttgtcgaaat gcagcacggt gtaatcaacg cctcgcacta tgcctataat gttgctgttg
     6661 atatcgatgc ccataattat cctgattacc tgctcacatg gggtaacttc gaaaagcaca
     6721 ttttttcgga gaagaatttc tttattcagc aggacaatgt gtatccggtc gggcatttct
     6781 atctggacta tatcaacagc cggaataaca ctgacgaagt gttgcaacag aagctatcag
     6841 gttataaatt aacgcttgca ttcactgcgc aggacgcttt cgaagaaaag gtgttacctc
     6901 ttattattga attggcgcaa cgcaaacccg aatgggcaat cgtatatatc ccgcgccgga
     6961 aaactgagga tcagtaccca acacttccct gcaacattat ttttgcatcg catttaaatt
     7021 gctaccagat aattgcacaa accgactggc atattacagt gagctcaact tgtgcacttg
     7081 aggcaccggc tatcgggaaa ccaaacgtac tgctcaattt cgagaaccgc tctcagctct
     7141 tttttggtgc tacattttcc ggcaaaacac acacacgatt tgtaagagat attgatgaac
     7201 tgatcacttc gctcgaaaat gattttattc ctaaccgtag taccatcatc gacgaatcaa
     7261 atgaattgtt tgcagccgat tttaaaaata atgtaataaa ttttatttca caggtttttc
     7321 ataaatgaag ctggggcaac ataaaatatt attcaagact gcgggtgtgt acaccttcgc
     7381 caatttcctg aatgcagcaa tacctttcct gttgttgcct ttcatgacca cgcacctgtc
     7441 gcccggcgat tacgggattg tggcaatgtt tcaggtgctg atgcaattta ccacgccctt
     7501 tgtgggaatg aatgccactt cagcattaac gcggcagtac tacgaaagag ataccatcaa
     7561 cattaaacag tatgtgtcgg atagcttctt tatcacgatg gttacgggga tttttattac
     7621 cctgctgttt atactgttta aaagtccgct ggcatcgatt actcaatttc ccgaaaattg
     7681 gctttttgta gtggtagtgt attgccttgc gcagaatatc ggactgatta tattgtcgtt
     7741 gtggcaggta cagtataagc cggtacctta tggtattttc aggattggac gtaccgtgct
     7801 cgatattaca ttttcggttt gtctgatttt attcgtagga atgaaatggg agggtatggt
     7861 gattggtcag acttctgcta ccattctact ttcgtttgtc gcgttgtttt ttctgcttaa
     7921 aggcaaatgg ttgacggtaa aagtgaacaa ggcatatgtg attgatgcat tgaaatttgg
     7981 tgtcccgtta attccacatg tggttggcgc tattattatc acatattccg atcgactatt
     8041 tatcaccaat atggtaaacc tcgaagaaac cggtttatac agtgttgggt ataatgtggg
     8101 aatgattgtt tatctggttc agaattcatt taatcaggca tgggtaccat ggttttacga
     8161 aaaattaaaa aacaacctca ttgccgataa aatcaaaatc gttaaattta cgtatgcgta
     8221 ttatgttgcg atggcagcga ttgtggcagt catcaccctg ggagcaccat ggttttttgc
     8281 cacttttctt ggtaaagatt tccacagcgc agtccaattt gttttttgga tagcacttgg
     8341 ctttgggttt aacggtatgt acaaaatggt tgtaaactat attttttacc tgaaaaaaac
     8401 atatattatc agcattatga ctgtggtaac tgctgtaata aatattgtgc tgaattattt
     8461 cttcataaaa agtatgggag ccataggtgc tgcaaaagca acggctgttt cgttttttat
     8521 cgaatttatt gttgtgtgga ttatcagttc gcgattatat aaaatgccct ggttactgag
     8581 aattaaagaa tgataggaat actttcatat ttcccgacgc ttgctttcat accgcactat
     8641 gcatttgcgc ttacttttat ttatgctgtc attcgaagga aagatattgt aattaatcac
     8701 ttttcgagtt tgcaagccat taaaagcaat tttctgctac tgttgctcat tgcttcactg
     8761 agtgttgcaa actgctttat cggagttgtt ttttatggaa cagagccggg tttcccatat
     8821 ccggtactca tgcttgccac ctactttatt gcattgtcgt tgtcgaaaac cgacctgaaa
     8881 tttattttct ggctggctgt gatagaatcg cttgtggttg tagctgaatt tgcaagcgga
     8941 acctccagtt ttattcctgc ttatgccagt atggataatc ccgacactga tctgctgtac
     9001 ttttcgagac ccatgggctt gagctataat tcaagtattg tcgcctacaa actgctggta
     9061 gcaattttaa ttgccgagta tctcgacaaa cgaactcttc tgtatcggtt ggctcaggtg
     9121 gtgctgctgg caggaatcat tctcactttt tcgagaacaa ttcttgtggt tttggtaatt
     9181 tactttgcaa ttcgatattc acggcagtac ctgattgcat ttgttgaact cgtaaggttc
     9241 aggatcagat acacaaatct tgtattcctt tttgcattca gtgcttttat tgtggtatcg
     9301 ttttattttg cctatacaaa tttttcttcg ataagcacac agtttacgcg cgggcgtggc
     9361 aacatcgaat tttcgggaag agaaaaaatc tggcctcagt ttattgactt tatcaaaaaa
     9421 aatcctttgg caggaaacca ttcgctcaag ttttatgccg attaccatgg gatacccgac
     9481 tctgcccatg cacacaattc gttcctgcag gtgcttgccg ataatggcat tttcattttt
     9541 gcgctgtaca tgatatttgt tttcgcaaat attaaccgga aaaatgtgta ctacatttta
     9601 ccgatactca tttacagtat aacacagtat ggagtgttct ggggaatatc gctacttgat
     9661 attgtattca tgatgatttt gttgcgcaaa gacaaagaat taacgatgct gacttgtaaa
     9721 cccattcaaa tttcatgaaa ataatactgg taaaccatta tgccggttca caatcggaac
     9781 ctaccttctg caaatacaga caaaacataa tgtaattacc caacgtataa tcaaaaacta
     9841 aggaatcatg aaacaacttc ctcccgcccg agccatcggc acgggattcg gagtaggagg
     9901 tgaatagcga attataaaaa tcattacaaa atcactaatt accataaaga acaaagcgag
     9961 acaatggcaa atgccagatg tcaaaggttt ataagcaatt aaagaacaat cgtgtttcag
    10021 atggcaaaag tcgctctcct cagtagaacc cacatttgac ttttggcatc agacttctga
    10081 catgcaatat tacttcttca aaaggaaccc ggaaggtgga tttcattatt gccgataaaa
    10141 tattgataac gaaagctaca aaattaattc aaatcaatta aaatagtttt ctcttgaaca
    10201 ttcatattta ttatttttac ataaagctta tgttatgaaa ctaatttgtt tattctcttt
    10261 acaattaata ttcttgatta attattcctt tgcccaaacc gaatggatta cggagatacc
    10321 tgttttcggt gatgcatgct caatgttacg tacaaaagat agctgttacg ccgttctaaa
    10381 atgccctatt tattacaacg ctgattccac aaactacttt tacgacctgc agcttagtaa
    10441 agtaacgcca cagggcgaat tgctctggta cgacactgtt ttttccgata ccttggattt
    10501 tgttgatctt agctttatcc aactttcgaa cgaagattat tttattaccg ggcaatgctg
    10561 gtgttgcccg ttttattgca ttgtttcaaa agaaggagaa ttaaagtatt tcgacaggct
    10621 cgaaaattac atggggacat atagtagttt cttttcccct atagagatat ccgatactga
    10681 ggtatgggtt ctgggtgggt attcacaaat gtatatcact aaaattaacc ttaccaacca
    10741 tgaagtaagc gttgattcca gtgctttatg cattcataac gcgagctggc cattaatgga
    10801 aagcttatct gataatagaa ttcttgtagc ttcaaattat ttaacaaata tttatataat
    10861 tgatattgct caaaatgtta ttaatcaaac ctcgttaaat tctaggtttt ttaaccctcg
    10921 ttataaacca ttccacatta atccgtatga cagcctttat tatttctacg tacaaacact
    10981 tgatccgtat cccaatatca ggtatggctt tatggtactc aacagccagc tcgattcaat
    11041 aaaattcatc gataacaccg aattctatcc caaccaatcg caggtagaat atccgagaat
    11101 attggatttc gattttccaa aacccggtaa catcaccatg accgggtata tctattggta
    11161 tcctggatat agtgaggaat tgccatatat ccatagctat aactacatcg aagataccga
    11221 cgagtatttc catgcctatg tcgggtcggt caccaggtat agcatcaaca tgctaaaaga
    11281 cggcaatggt tacgtaatgc tccatgcatt ccagggcaat ccgggtttcg tgtttttggt
    11341 aaaagaaaac accgataccc tcacaaaccg ggtacccgac ctggaatatg acactgctgt
    11401 aaaaatctat ccaaacccgg caggcgctta cataacaata gaaagcaata aaacgacttt
    11461 cagcgaaatc atcatactca acgccgtggg gaacacaata agcgacataa catttggaga
    11521 aaccaggcaa aaagaaatat ccgtcgaaaa acttgctccc ggcaggtatt tcataaaaat
    11581 acgtaataat aaaaaccaaa cgcatattac atcattcgta aaatcctgaa cctatgaaaa
    11641 acaagtttta ctgcataata atattatttg cggctttgct gccaaccgcc attccggctt
    11701 attctcaaaa ctcatgaata accaaaatac ccacattgct tctataaaat taataattag
    11761 aaagtacttt ttatttttta ctaaattttc atgtgttatt atattggttg tttgtaatag
    11821 caattgtttt gctcaatggg atgatattac ccctttaaat ggtttatata acaaagtttt
    11881 attcatcaat caggacactg gttttgttat tgctaattat ggcttaataa taaaaacata
    11941 tgattttggt ttgacttggg atgttgtgag tcaaaataat ggatacaact acaaggatat
    12001 atgttttaat aataacctgt gttttatatt agggtccaat gaaaatttaa attctcaaat
    12061 atttattaaa tcagccgatt atggacaaag ttgggataca atagtattgc caaataatca
    12121 tggtgaatca tttcttttta ttaatgattc aacagcatta atagttggcg ataatatttt
    12181 aagaacaaca gattattgta atagttttga atcagttatg gagttttcaa atattggtgc
    12241 attctatggt ggattattat caattgcttt tgtaaatgat agtgttggat ttgcctcagg
    12301 aataaaacgc gcaaattaaa ctactcccca ttccttagac cacttttttg tattcttaat
    12361 tgaaatcttt a
//
LOCUS       DAOOQP010000085        12240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6411_length_12240_cov_6.196553, whole genome shotgun sequence.
ACCESSION   DAOOQP010000085 DAOOQP010000000
VERSION     DAOOQP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6411_length_12240_cov_6.196553"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLK75_09140"
     CDS             <1..1116
                     /locus_tag="PLK75_09140"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOY39450.1"
                     /translation="VLHTIPSGIFRENKVNIIGNGVVLDPISFRGELQATAKQGGFVY
                     ENLFISRKAHLILPTHRMLDAANEKLKGNDKIGSTLKGIGPAYTDKTARIGLRVGDIE
                     LPGFREKYNRLRDHHQALLQSMKFDYAIESEETDWFSAIEDMKRIKFIDSEYVINKAL
                     ADGKTVLAEGAQGSLLDVDFGSYPFVTSSNTCCSGVCSGLGIPPSAIGEVIGVFKAYC
                     TRVGSGPFPTELFDKTGAQLRDNGREYGSTTGRPRRCGWLDLVALKYSTMLNGVTKLH
                     MMKADVLDEMHSIKVCVAYKLHDRIIDYIPYDINEQITPVYKELQGWNCDITSLKNAN
                     HVPEALQEYIRFIENELKIPVVGLSVGPNRNQTLKLR"
     gene            1275..2219
                     /locus_tag="PLK75_09145"
     CDS             1275..2219
                     /locus_tag="PLK75_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY39451.1"
                     /translation="MIRTGIIGSEQIWQGYVESINNIGELRLVGFFDPAADSTIRELS
                     GVKPFAESDSLLHEVDAVIALSPMSSAEHIENYVRNSKHVLFEPVQLFSKSEASRLMN
                     MIDEADVKVQPGFDKRYNMVFRAVRPFIKNPRIIVSNHFVQYDVKTRNTSVLIDILLN
                     DIDLVLSLVNSEIKSISATGVSINNTSPDTINARIEFNNKCVAQFNAGRVALEEKNEI
                     TFYHNNNYVYANLSANSAMLVRKNAPFTDLGLFSSLKGELQCEPILIPSTDEKYSSIK
                     AFASAIVKNTSPEVNLESMMRTFSAAQIIAEKLKLVSH"
     gene            2243..3175
                     /locus_tag="PLK75_09150"
     CDS             2243..3175
                     /locus_tag="PLK75_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39452.1"
                     /translation="MPAIIPCILSLYVFTAKGSLMKLFYVAITFILLAAAMSCEIINP
                     PEEIPSYISVDSVVLNTNYSTQGSRVENITDAWVYVDDKLVGVFEIPFTVPVLASGQS
                     EIEIFPGIKNNGIAAERLIYPFMNFYSDNVELTPAEILKITPHFSYKDVTFALLEDFE
                     DLGIAFQVSEQSDTSIMLVTGPDAMEGNSMFFALDSVRDVFECRSTELFELPLAKAVY
                     LEMSFKTTDYFTFGLFARKYSNGSSIEIRDPIITFNPTSEWKTIYIDLSDCINDNSDA
                     FDFRLYYTCARSDDQENDTTRVYIDNVKLIYQQL"
     gene            3172..4587
                     /locus_tag="PLK75_09155"
     CDS             3172..4587
                     /locus_tag="PLK75_09155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOY39453.1"
                     /translation="MNKAKQLIKYICFDLLAAGLSYYLFYLFRKVYIEPSKFGYDIPF
                     EISTQFIIGLIFIPIFWIFLHLLSGYYRNPLRKSRLHELWMTFLTTLIGMLILFFVLL
                     LDDWVGNYKQYYITFSVYFGLQFITTYIPRLVITSRTNHRIHTRKLGFNTIIVGSGRK
                     AVKLFQEIENQAVSIGFKFLGFLSIDSKDTVLLDKHLPHLGTIKDIKQIIADNKIEEV
                     VIALESKEHGTIKNIINQLQGLMVEIQVIPDMYDILIGKVRMSAILGVPLLQIRHNLM
                     PVWQLHTKRFIDVFISCLALLLLSPLYLFLAIGVKLSSSGPVIYSHERIGKNGKPFTI
                     YKFRSMQKNAEKNGPELSNKSDNRVTNFGKFMRKTRLDELPQFFNVLKGDMSIVGPRP
                     ERKYFIDQIVKVAPHYVHLLSVRPGITSWGQVKYGYAENINQMVDRLKYDILYIENMS
                     LYVDFKIMIYTVKIIFQGKGI"
     gene            complement(4646..5704)
                     /locus_tag="PLK75_09160"
     CDS             complement(4646..5704)
                     /locus_tag="PLK75_09160"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOY39454.1"
                     /translation="MKFRDTLYQWYELHRRTLPWRETDDAYRIWISEIVLQQTRVIQG
                     LGYYERLIASYPDVFSLAAASEDDVYKHWEGLGYYNRAALMLKTAREIANNYNGVFPT
                     TSAELIRLKGIGEYTAAAIASFAFGEAVMCVDGNVKRLISRYFGIESLYGSSRFETEV
                     CTSLSAVFDKENPALFNQAIMEFGALQCKPKAVCPTCPFLNECFANMHGKQNSIPVKP
                     IKKTVATRYFNYFVVLSDDMKTLIKKRTADDIWKNMFEFPMTETIGPISDKILLPTDI
                     KAVCKSTQASLLFEAAIHKLTHQQIAARFYVIRTSVVLPIRKKTDLLLVDSVDLEQYA
                     MPRIITKYLPQIRESIFI"
     gene            5830..6111
                     /locus_tag="PLK75_09165"
     CDS             5830..6111
                     /locus_tag="PLK75_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOY39455.1"
                     /translation="MTKSDIVNEISKNTGIEKQMVQHTVEAFMESLKKSLIKGNNVYL
                     RGFGSFIIKQRAEKTARNISKNTTITIPAHRVPSFKPSKTFINQVTENN"
     gene            6273..7859
                     /locus_tag="PLK75_09170"
     CDS             6273..7859
                     /locus_tag="PLK75_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOY39456.1"
                     /translation="MKNSNVEKQSEELKNELYIDVRPSEITIALLQNKQLVELNREVN
                     NTKFSVGDIYLGKVKRIMPGLNAAFIDVGYEKDAFLHYLDLGPQFQTFNKFVQQIISK
                     KQKKPTFASFVPEADINKNGKIGDILNPGQFIMVQIAKEPISSKGPRLSAEISIAGRN
                     LVLIPFSDRISVSQKIKSNEERKRLKQLIQSIIPKNFGMVVRTVAETKKVANLDRELR
                     QLVQRWDTILSKLPTAAAPSLLIGEDDRATVILRDILNASFNQIVVNEAGTYEDIRAF
                     ISEIAPEKQKIVKFYNQKPPIFDHFGIEKQIKTLFGKTVMLKNGAYLIIEHTEAAHVI
                     DVNSGNRSSTDENQEMNATEVNLLATEEIARQLRLRDMGGIIIIDFIDMHDGTNKIKI
                     LEKMKASMAGDRAKFHILPLSKFCLMEITRQRVRPEMYIKTAETCPTCSGSGKIGASI
                     NLVDEIENNLKFILKKYSFSKICIKTHPFVATYLNKGWFSIVSKWRKQYKQKLVVEAM
                     QEYTYMEYHFFNDLDEEIIY"
     gene            7871..9916
                     /locus_tag="PLK75_09175"
     CDS             7871..9916
                     /locus_tag="PLK75_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOY39457.1"
                     /translation="MKHRFRIFLSSLFLLIASFSFSQITEPVTWTFSTEKVSEKELYL
                     VLTAKIENGWHLYSQYFDDGGPIPMVISFEKNQNYITVDSTLEVGKAIDEYDDVFMLN
                     TKYYKGIVVFKQKIQVNSTEKFIIEGSLAGQACQDDGMCVPVNADFKFGINGADANAE
                     SGSTSEAIASKDLPQTPDIVSENTYKNTEKKENLFWFFLISFLAGLAAILTPCVFPMI
                     PMTISFFMKENSKSKARFNAFAFGISIILIYTLIGSVLAIIFGPSIANWLSTHWIPNI
                     LFFLIFMIFAASFFGAFEIVMPSWLVNKSDSKADRGGVLGAFFMALTLVLVSFSCTGP
                     IVGSILVESFGGSIVKPIIGMLGFALAFALPFTLFALFPGWLKNMPKSGGWLNTVKVV
                     LGFIEVALGFKFLSIADQTYHWGLLDRQVYLAIWIVVFALMGFYLLGKLRFKHDDEPK
                     YLSTGRLSLAIVTFVFVLYMIPGMWGAPLKALSGYLPPITTHYFNIPQIIADNNRLML
                     QKMGIEEENQTCEEPKYKEQLHLPFGLHGYFDWKQAFDCAKQQNKPVFIDFTGHGCVN
                     CREMETAVWSDSRVIELLRNDYIILAMYVDDKTIELPEDEWFVSKNDKKVKKTLGDAN
                     ADIQIVNFDRNSQPYYVLISPEGVVLNEPRAYDKNADEFVKFLQKGLENFEKLNKSE"
     gene            complement(9938..11548)
                     /locus_tag="PLK75_09180"
     CDS             complement(9938..11548)
                     /locus_tag="PLK75_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY39458.1"
                     /translation="MKETFEDIIKKSLENYSPAAPEHLLKKLQTSYPKPGFRDFVSLH
                     SIQISVILGILISGIIALVIFTNNTKNNSPQTADSNNSSNNETINTNIRERDNSMKPS
                     PVAVWPKKSNSVNTFVPSDKNKTVDTRIFTCSDTSVCGNEITISTVVSVDKLFIDHSC
                     KIEETNANLFRITASIPGVYYLKHLNSGHSTDSMRICFTGTAEPRIKIVENVVCAGEP
                     LVVMVDNANSRELFWDIENATVESAGNNLYKIVFKSYEGQKVKISVSARNAGCSCNTC
                     IEYQLPEKLQVKTSVEPEICSNSNGVIHIKTNATGAHYLLNNTKENSDGTFRNLKHGN
                     YNVSVIYNNGCFENFNITIPANKQLKASFTYNTDPANSSRLLVTNTTSIDNKGYTQFG
                     DIDFTWIANNETYYSDNPTLEFSSSGKQEVILVASYGESCKDTCRTAIQISNDEVLIP
                     NIFSPNGDGISDIFFVKASNVVSFRGIITNIRGEVVFNWDNISEGWDGKINGNNLASE
                     GVYYYLIGIEFTNGNKVEKKGSLNLIRK"
     gene            complement(11532..12167)
                     /locus_tag="PLK75_09185"
     CDS             complement(11532..12167)
                     /locus_tag="PLK75_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY39459.1"
                     /translation="MLPKKYGSIFFIRQRFICYVCLINATAKLENERLIKQCQKKSKK
                     AFDELYRTYSGMIYGICMRYTKNSAEAEDLLHDCFIKVMNKIGEFEFKGSFEGWLKRI
                     AVNSAINNIKAKKLVYVDDLETETKETNCPESLGIIQSMDCKQIIELINTLPVGYKTV
                     FNLYVVEGYKHKEIGEMLGFTENTSKTQFLKARKALMEMLSKLKEEDERNI"
BASE COUNT         3702 a         2331 c         2426 g         3781 t
ORIGIN      
        1 gtattgcata caattccctc gggtatcttc agggaaaata aggtaaatat cattggcaat
       61 ggggtagtac tcgacccgat aagtttcagg ggtgaattac aggcaacagc taaacagggt
      121 ggatttgttt acgaaaactt gtttatcagc agaaaagcgc atttaatttt accgacccat
      181 agaatgcttg acgctgcaaa tgagaaatta aaaggaaacg ataaaatagg gagtactctt
      241 aaaggcattg gccctgcata tactgataaa actgcacgta ttggactacg ggtgggcgat
      301 attgagcttc caggtttcag ggagaaatac aacagacttc gcgaccatca ccaggccttg
      361 ttgcagtcca tgaaatttga ttatgccatt gaaagcgaag agaccgattg gtttagcgca
      421 atcgaagata tgaagcgtat aaaattcatc gatagcgaat acgttattaa taaagccctt
      481 gcagatggta aaactgtact tgccgaaggt gcacagggaa gtctgttgga tgtagatttc
      541 gggtcctacc cgtttgttac ttcatcaaat acatgttgtt ccggtgtatg ttcaggccta
      601 ggcatcccac cctctgcaat cggggaggtc attggtgttt ttaaagccta ctgcacacgt
      661 gttggaagtg gccctttccc taccgagttg tttgataaaa caggagcaca gctgcgtgac
      721 aatgggcgtg aatatggatc caccaccggt cgtcctcgcc ggtgtggatg gctcgacctc
      781 gttgcactaa aatattcgac catgctcaat ggggttacaa aattgcatat gatgaaagcc
      841 gatgtactcg acgaaatgca tagtattaag gtttgtgttg catataagtt acatgacagg
      901 ataatcgatt acattcccta cgatatcaat gagcaaatca cacctgtgta caaagagctt
      961 cagggatgga attgcgatat aacctcgtta aaaaatgcaa atcatgtccc ggaagcatta
     1021 caagaatata tccggtttat tgaaaatgaa cttaaaattc cggttgttgg attgtctgtt
     1081 ggtcctaaca gaaatcagac actgaagctc cgttaatcat tggtgaactt caaaaatata
     1141 tgaagttttt gttgaataca catgtctatg tacatgataa tcagtgctat taacacgaca
     1201 gggttaatat attccgacgg atcgccttat ttcacggcat aaatacattt atttttatcg
     1261 gaaaatctgt tcaaatgata cgtaccggca taatcggaag cgaacaaata tggcagggtt
     1321 atgttgaaag catcaacaac attggcgaac tgcggcttgt tggctttttc gacccagctg
     1381 cagattcaac aataagagaa ttgtcaggtg ttaaaccttt cgcagaatcc gactctctgc
     1441 ttcacgaagt tgatgctgta attgccctgt cgcctatgtc atcggctgag catatcgaaa
     1501 attatgtgcg aaattcaaag catgttcttt ttgaaccggt gcaattgttc agtaaatcgg
     1561 aagccagccg cttgatgaat atgattgatg aagctgatgt gaaagtacag cccggtttcg
     1621 acaaaaggta caatatggtt tttcgtgcag tacgaccatt tattaaaaac cccagaataa
     1681 ttgtttcaaa ccattttgta cagtacgatg tcaaaacacg gaacacctca gtattgattg
     1741 atatattatt gaatgatatt gatttagttc tcagcctggt gaattccgaa atcaaaagca
     1801 tttcagcaac cggtgttagc attaataata ccagtccgga tactattaat gcacgaattg
     1861 agttcaataa taaatgtgtt gctcagttta atgccggaag agtagccctt gaagaaaaaa
     1921 acgaaatcac tttctatcat aacaacaact atgtttacgc taacctttcg gcaaatagtg
     1981 ccatgctggt aaggaagaac gctcctttta ccgatctggg acttttcagt tccctcaaag
     2041 gagagttgca atgcgaacct atacttatac cttcaaccga tgaaaaatac agcagcataa
     2101 aggcatttgc cagtgctatt gttaaaaata cgagcccaga ggtgaacctt gaatcgatga
     2161 tgcgaacttt ttcagcggcg cagataattg cggaaaaact taagttagtt tcgcattgat
     2221 tatttgctta taaattttat ttttgccggc aataattccg tgtattttat cgttgtatgt
     2281 ttttactgcg aaaggaagtt tgatgaagtt gttctacgtt gccattacat ttattttgct
     2341 tgctgcagca atgtcgtgcg aaataattaa tcctccggag gaaataccct cctacatttc
     2401 ggttgacagc gtagtgctta acactaacta tagtacccag ggcagtaggg tagaaaatat
     2461 tacagatgca tgggtttatg tcgacgacaa gctcgtcgga gttttcgaaa taccttttac
     2521 cgttccagtt ttggcctcag ggcaaagtga aattgaaatt tttccgggaa ttaaaaacaa
     2581 tggaattgca gctgaacgtc tcatttaccc ctttatgaac ttttattcgg ataatgtgga
     2641 acttacccca gccgaaatat tgaaaataac accacatttt tcgtataagg atgtaacttt
     2701 tgctttgctc gaagattttg aagatttggg gatagcgttt caggttagcg aacaaagcga
     2761 cacctcaata atgctggtaa ctggtcccga tgccatggaa ggcaattcaa tgttttttgc
     2821 actcgactcg gtgcgtgatg tttttgagtg ccgctctacc gaattatttg aactacccct
     2881 tgcgaaggcg gtatatcttg aaatgagttt taaaactacc gattatttta cttttggatt
     2941 atttgcccgc aaatacagta atgggtcttc aattgagata agagacccga ttattacttt
     3001 caatccaact tcggagtgga aaactattta tatcgacctg agcgattgca tcaacgataa
     3061 ttccgacgca tttgatttca ggctttatta cacctgtgca cgttctgatg atcaggaaaa
     3121 cgatactacc agagtatata tcgacaatgt aaaactaatt taccagcaac tatgaacaaa
     3181 gcaaaacagc tgataaagta catttgtttc gatttgcttg ctgccggttt atcgtattac
     3241 ctgttctatt tgtttcgcaa ggtgtatatt gagccatcaa aattcggata cgatattcct
     3301 ttcgaaatca gcacacagtt tattataggg ttaattttta tacccatttt ctggatattt
     3361 ctgcatttac tgagcggata ttaccgtaac ccattgcgca aatcgcgcct tcatgaattg
     3421 tggatgactt ttcttacaac gctgattggg atgctgattt tattttttgt tttgcttctc
     3481 gacgattggg ttggcaatta caagcaatac tacattactt tctcggtata ttttgggctt
     3541 cagtttatca caacctacat tccccgcctt gtgattacga gcaggaccaa tcaccgtatt
     3601 catacccgta agctgggatt taatacgatt attgtcggtt caggacgtaa agcagtaaaa
     3661 ctttttcaag aaattgaaaa tcaggcagtt tctataggat ttaagttctt aggatttcta
     3721 agcatcgatt caaaagatac ggtattgttg gacaagcatc tgcctcacct tgggacgatt
     3781 aaagacataa aacagattat tgctgataac aagattgaag aagttgtaat tgcactggaa
     3841 tcgaaagagc acggcacaat taaaaacatc attaatcagt tgcagggttt aatggtcgaa
     3901 atacaggtaa ttcccgatat gtacgatatt cttattggta aagttaggat gagtgctatt
     3961 ctgggggttc cccttttaca aatcaggcac aacctgatgc ctgtttggca gttgcataca
     4021 aaacgattta tcgatgtttt cattagttgc cttgccttgc tcttgctatc tccgttatat
     4081 ttgttcttgg ccatcggtgt taagctttcc tcttccggac ctgttatata cagccatgaa
     4141 cgaattggta aaaacggaaa gccgttcact atttataaat tcaggtcgat gcagaagaat
     4201 gccgagaaaa atggacctga gcttagcaat aaatctgaca accgggttac aaattttgga
     4261 aaattcatgc ggaaaacacg cctcgatgaa ttgccccagt tttttaacgt acttaaaggt
     4321 gacatgtcga ttgtgggccc acggccggaa cgcaaatatt ttattgacca gattgtaaaa
     4381 gtggcgcctc attatgtaca tctgctgagt gttcgtcccg ggatcacatc atgggggcag
     4441 gtgaaatacg ggtatgccga aaacattaat caaatggttg atcgcttgaa atacgatata
     4501 ttatacatcg aaaatatgtc gctatatgtc gattttaaaa taatgattta cactgttaaa
     4561 atcatatttc aaggcaaggg gatttaaaat aaaacactta atcacaaggg gatagagcct
     4621 tattccttga ctactggaat tgccttcaga taaaaatact ttctctgatt tgaggcaaat
     4681 attttgtaat tattcgtggc attgcatact gctccaaatc aactgaatca accagcagta
     4741 agtcagtttt tttacgaatt ggcaatacaa cagatgttcg tattacgtaa aatcttgctg
     4801 caatttgttg gtgagtgagc ttgtgaatgg ctgcttcaaa aaggagagat gcttgtgtcg
     4861 acttgcaaac agccttaata tctgttggta ataaaatttt atcacttatt ggtccaatgg
     4921 tttcagtcat cggaaattca aacatgtttt tccatatgtc atcagcagtt ctttttttta
     4981 ttagggtttt catatcatca ctaagtacaa caaaataatt gaagtatcgc gtggcaacag
     5041 ttttcttaat tggtttaacg gggatactgt tctgtttccc gtgcatgttg gcaaagcatt
     5101 cgttcaaaaa ggggcaggtt gggcaaacag cttttggttt gcattgaagg gctccaaatt
     5161 ccattattgc ctggttgaat aatgcagggt tttctttatc gaatactgca gacaaagagg
     5221 tacaaacttc agtttcaaag cgtgaagatc catacaacga ttcaatccca aaatacctcg
     5281 agatcagtct tttaacattt ccatcaacac acattaccgc ttcaccaaag gcaaacgatg
     5341 caatcgctgc cgcagtatat tctccaatgc ctttaagcct tattagctcc gcagacgtag
     5401 taggaaatac tccattgtag ttatttgcaa tttcccgggc tgttttcagc atcaatgcag
     5461 cgcgattata ataacccagg ccttcccaat gtttatacac atcatcctcg ctagcggccg
     5521 ctaaactaaa tacatccgga taagacgcaa taagacgctc ataatatccc aaaccttgaa
     5581 ttactcgtgt ctgctgaagc acaatctccg aaatccagat tcggtacgca tcgtctgttt
     5641 cccgccatgg aagagtgcgc cggtgcaact cgtaccattg atatagtgta tccctgaatt
     5701 tcaatataat gattttaatt aattaacaac attactattt atttgttttg caaataaaaa
     5761 aaactactta tatttgcacc tcttttaatt gacttatcta atttttaata ttataaaaaa
     5821 cactgagaaa tgacaaagtc ggacattgta aatgaaattt ctaaaaacac aggtatcgaa
     5881 aaacaaatgg tacagcacac tgttgaagct tttatggaaa gtttaaaaaa atcgcttata
     5941 aaaggtaaca atgtttatct gagaggtttc ggaagtttca tcattaaaca aagagcagaa
     6001 aaaacagcac gcaatatttc gaagaatacc acaattacaa ttcccgcaca cagggttcct
     6061 tctttcaaac cttcgaaaac ttttattaac caagttactg aaaacaacta aaatattgta
     6121 tcatgcctag tggaaaaaaa cgcaagagac ataagatgtc gacgcacaag agaaaaaaac
     6181 gtcttcgcaa gaacagacat aagaagaaaa acaaatagca tcctgcatct tttaatcaaa
     6241 cctttaggat tcctaaaggt ttgatttgtt acatgaaaaa ttccaacgtg gaaaagcaaa
     6301 gcgaagaatt aaaaaacgaa ttatatattg atgtcaggcc ttctgaaatc acaatagcat
     6361 tacttcaaaa caaacaactg gtcgaactca accgggaagt caataacaca aaattttcag
     6421 tcggcgacat ctatctggga aaagtgaaac gcattatgcc cggattgaat gctgctttta
     6481 ttgatgttgg atacgaaaaa gatgcttttt tacattatct cgaccttggc ccacagttcc
     6541 aaacgtttaa taaatttgta cagcaaatta tttcgaaaaa gcagaagaaa cccactttcg
     6601 caagttttgt tccggaggcc gatatcaata aaaatggaaa aataggagac atccttaacc
     6661 ccgggcaatt catcatggtg cagattgcca aggaacccat ttcgtcgaaa ggaccaagac
     6721 tgagcgctga aatttctatt gcaggtcgta atctggtact tattccattc tccgacagaa
     6781 tttcggtaag ccagaaaatc aaatcaaatg aggagcgcaa acgtttaaaa caactgatcc
     6841 agagtattat accgaaaaat tttggtatgg tggtcagaac ggttgccgaa acaaaaaaag
     6901 tagccaacct cgacagagaa ttgcggcagc ttgtgcaacg atgggatacg attttgagca
     6961 agcttcccac ggcagcagcc ccaagtttac tcattggcga agacgaccgc gctacagtaa
     7021 ttttgcgcga tattttaaat gcttctttta atcagattgt cgttaacgaa gccggcactt
     7081 acgaagatat cagggctttt atcagtgaaa ttgctcccga gaagcaaaaa attgtaaaat
     7141 tttataatca gaaacccccg atttttgacc atttcggaat cgaaaagcaa attaaaaccc
     7201 ttttcgggaa aacagtgatg ctcaaaaacg gtgcttacct cattattgaa cacaccgaag
     7261 ctgctcacgt aattgacgtg aatagcggaa accgttccag tacagacgag aatcaggaaa
     7321 tgaatgcaac cgaggtaaac cttcttgcaa ccgaagaaat tgcccgtcag ttacggttgc
     7381 gcgacatggg aggcattatt attatcgatt ttatcgatat gcatgatggc acaaataaaa
     7441 tcaagattct cgaaaagatg aaggccagta tggctggcga ccgggccaag tttcatattc
     7501 taccactcag taagttctgt ttaatggaaa ttacaaggca acgtgttcgc cctgagatgt
     7561 acattaaaac tgctgaaacc tgcccgactt gctcaggttc tggtaaaatt ggtgcgagca
     7621 taaatctggt tgatgagatt gaaaacaacc tgaaatttat tctaaaaaaa tattcattca
     7681 gcaaaatttg cattaaaaca catccatttg ttgcaaccta tttaaacaaa gggtggtttt
     7741 cgattgtgag taaatggcgt aaacaatata aacaaaaact tgttgttgaa gcgatgcagg
     7801 aatataccta catggaatat cattttttta atgatctcga cgaagaaatt atttattgaa
     7861 tttataacat atgaagcata gatttagaat ttttctgagc tcactgtttc tccttattgc
     7921 atccttttct ttttctcaaa ttactgagcc tgttacatgg acattcagta ccgagaaagt
     7981 aagcgaaaag gagctatatc tcgttctcac agccaaaata gaaaatgggt ggcatttgta
     8041 ttcgcagtat ttcgatgatg gtggtccaat tccaatggtg ataagcttcg agaaaaatca
     8101 gaattacatc acggttgata gtactctgga agttgggaaa gccattgatg aatacgacga
     8161 tgtgtttatg ttgaatacga aatactacaa aggtattgtt gtgttcaaac aaaaaataca
     8221 agtgaattcc actgagaagt ttattattga aggcagcctt gccggacagg cttgtcagga
     8281 tgatggtatg tgtgtacctg tgaatgccga ttttaaattt gggataaacg gagccgatgc
     8341 aaatgctgaa agcggttcca catcagaagc aatagcatca aaagacctcc cgcaaacacc
     8401 tgacattgtt tcggaaaata cctataagaa taccgaaaaa aaggaaaacc tgttttggtt
     8461 ttttctgatt tcattccttg caggattggc agcaattctt acaccttgtg ttttcccaat
     8521 gataccgatg acaatttcat tctttatgaa ggagaatagt aagtcgaagg ctcgtttcaa
     8581 tgcttttgct tttggtatca gtattatcct tatttacaca cttattggat cggtacttgc
     8641 aattattttt ggaccatcca ttgcaaactg gctgagtaca cactggattc caaacatact
     8701 gttcttcctg atttttatga tatttgccgc ttcgtttttt ggagcattcg aaatagtgat
     8761 gccctcatgg cttgtgaata aatcggattc caaagccgac agaggaggtg ttcttggtgc
     8821 gttttttatg gctcttactc tggttctggt ttcgttttcc tgtactggac ccattgtcgg
     8881 gagcatactt gttgaatctt tcggaggtag tattgtaaaa cctattattg gaatgttggg
     8941 ttttgcactg gcttttgccc tcccatttac actttttgct ctcttcccag ggtggctgaa
     9001 gaatatgcca aaatccggag gctggctcaa cactgttaag gtagttcttg gctttattga
     9061 agtagctctt ggttttaaat ttctcagtat tgccgatcag acctatcatt ggggtttgct
     9121 cgaccgccag gtttaccttg caatttggat tgttgttttc gcactaatgg gattttattt
     9181 gctgggaaaa cttcggttta aacacgatga tgaacccaaa tatctgagta ccggcaggct
     9241 gagtctcgcg atagttacat ttgtttttgt tttatatatg attcccggta tgtggggcgc
     9301 accacttaaa gcattgtcag gatatcttcc tccaattaca acacattatt tcaatatacc
     9361 ccagatcatt gctgacaaca ataggttgat gcttcaaaaa atgggtattg aggaagagaa
     9421 tcaaacatgt gaagaaccca agtataagga acagttgcac ctccctttcg gactgcatgg
     9481 gtatttcgat tggaaacagg cttttgattg cgcaaagcaa caaaataaac cagttttcat
     9541 tgatttcaca ggccatggat gtgtgaattg ccgagaaatg gaaacagctg tgtggagtga
     9601 ttccagggtg attgaattac ttaggaacga ttatataatt ttggctatgt atgtcgatga
     9661 taaaaccatt gagctgcccg aggacgagtg gtttgtctcg aaaaatgata agaaagttaa
     9721 gaagacactg ggcgatgcca atgccgacat tcaaattgta aacttcgacc gtaattcaca
     9781 accctactat gtgctgattt ctcctgaggg agttgttcta aatgaaccca gagcgtacga
     9841 taaaaatgcg gatgaatttg tgaagttctt gcaaaaaggg ctggagaact ttgagaaatt
     9901 gaataaaagc gaataaggac agactgagtt actgaaatta ctttctgatt agattcaggc
     9961 tgcctttttt ctccacctta tttccgttcg taaattcaat gcctatcagg tagtaatata
    10021 caccttccga agctagattg ttaccgttta tttttccatc ccatccttct gaaatattat
    10081 cccagttgaa tacaacctct ccacggatat tggtaataat tccgcggaat gatacaacat
    10141 tagaagcttt tacgaagaaa atatcggaaa tgccatcacc gtttggtgaa aaaatattag
    10201 gaatcaacac ttcatcattc gaaatctgaa ttgctgttct gcatgtatct ttgcatgatt
    10261 ctccatacga tgcaaccaaa ataacctcct gcttcccgct gctcgaaaac tccagtgtgg
    10321 ggttatcgga ataatatgtt tcgttattgg ctatccacgt gaaatcgatg tcaccaaatt
    10381 gtgtataacc cttgttatcg atgctggtag tgttggtaac taataacctg cttgaatttg
    10441 cagggtcggt gttgtaagtg aacgatgcct taagttgttt atttgcaggg atagtaatat
    10501 tgaagttctc aaaacaacca ttgttgtata tcaccgatac attgtagttg ccgtgcttaa
    10561 gattgcggaa ggtcccatca ctgttttctt tggtattatt cagaaggtaa tgcgctcccg
    10621 tggcgttggt tttaatatga attaccccat tgctgttact gcatatttca ggctcaaccg
    10681 atgtttttac ttgcaatttt tcgggtaact gatattcgat acatgtgtta caactacaac
    10741 cagcatttct tgcggaaaca gatattttaa ctttttgacc ttcgtatgat ttaaaaacaa
    10801 ttttgtacaa attgttgccg gctgattcaa ctgtggcatt ttcaatatcc caaaacaatt
    10861 cccggctgtt tgcattatca accattacta ctaagggttc ccctgcgcag acaacgtttt
    10921 ctacaatctt gatcctgggt tcagcagttc ctgtaaagca tattctcata gaatcggtag
    10981 aatggcctga attaagatgt ttcaaataat aaacccctgg aatagaggca gtaattctga
    11041 ataaattagc atttgtttct tcaattttgc aggagtgatc aatgaaaagc ttatccacac
    11101 taactactgt gctaatagtg atttcattac cgcaaaccga tgtgtcggag caggtaaata
    11161 tgcgggtatc aacagttttg tttttatccg acggaacaaa tgtgtttaca gagttgcttt
    11221 ttttcggcca aactgcaaca ggggatggct tcattgaatt gtctcgttcc cgaatattgg
    11281 tgttgattgt ttcattattc gaagaattat ttgaatccgc agtttgtgga gagttgtttt
    11341 tagtattgtt tgtgaaaatt accaatgcaa taatccctga aattaagatt ccgagaatca
    11401 ccgaaatttg tattgaatgt aaagaaacaa aatcgcgaaa ccccggcttt ggatagctgg
    11461 tctgaagctt ttttaagaga tgctccggag ctgcgggaga atagttttcc agcgattttt
    11521 taattatatc ttcaaatgtt tctttcatct tcttctttca gtttactcaa catttccata
    11581 agggcctttc tggctttcaa aaattgtgtc ttcgaagtgt tttcggtaaa tccgagcatt
    11641 tctccaattt ctttatgctt ataaccctcg acaacataca ggttaaatac ggttttatag
    11701 ccaacgggca gggtgttgat aagctcgatt atttgcttgc aatccatact ctgtataatt
    11761 ccgagcgatt cggggcaatt tgtttctttt gtttcagttt ccagatcatc gacataaacc
    11821 agcttttttg ccttgatatt gtttatggct gaatttactg cgattctttt tagccaacct
    11881 tcaaacgaac ccttgaattc aaattcccca attttattca tcacctttat aaaacagtcg
    11941 tgtaaaagat cttctgcttc agccgaattt tttgtgtacc tcatgcaaat accgtaaatc
    12001 attcctgaat aggttctgta caattcatca aaagcttttt tcgacttctt ctgacattgc
    12061 tttattaacc tttcgttttc caattttgct gtagcattaa taagacaaac gtaacatata
    12121 aaacgttgtc tgataaaaaa aatgctgcca tatttttttg gcagcatttt taaaatttat
    12181 gcaattattt ttctctcatg aggtgcattg gtccctggaa ctcgtattcc acatcgtcga
//
LOCUS       DAOOQP010000086        11931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6652_length_11931_cov_4.597087, whole genome shotgun sequence.
ACCESSION   DAOOQP010000086 DAOOQP010000000
VERSION     DAOOQP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6652_length_11931_cov_4.597087"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLK75_09190"
     CDS             <1..404
                     /locus_tag="PLK75_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY39460.1"
                     /translation="NLKSVFNLTKAVQKIMLKQKNGSIINISSVVGVNGNAGQANYAA
                     SKAGIIGFTKSIAKELGSRNVRCNAIAPGFIETEMTAALPEDYRNTWNEKIALRRAGK
                     PEDVADVVVFLGSDLSSYVTGQVISVCGGMI"
     gene            450..2525
                     /locus_tag="PLK75_09195"
     CDS             450..2525
                     /locus_tag="PLK75_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39461.1"
                     /translation="MIAQYSWWWLALFLVLSVGMSMALYYRDKALAELQKWKILLLAL
                     LRFLSVFLILCLLFAPLLKTKKKIIEKPIIVFALDNSASVSRNTDSLYYSRQYANDIR
                     NLMSDLSTDYQVYQYNFGEQMRQDSIIDFSDKATDMSEIFPMIRAAHSGRNLGAVIVA
                     GDGIFNVGENPVYSTDIGTTVYTIALGDTVRSKDVFIKQVQHNKVAFYKNSFPVRIIV
                     EAVQCNNEKIRIRVLNVNEKLFEKEIIAYEDDCIEKIELELPASSKGLQQYEVVIDRI
                     EGEISTENNRQSFVVEVIDNKQNILILGNSPHPDIGAIAYALKSNPDYQISVQYAGTV
                     TESMNKYQLIILHQVPSVNNPAGNILNQISRNKIPCIYILGAKTSLDALNLTQSGLKI
                     TAAKSSFDEATPLFQSSFSAYSNDQEWIDIVSSLPPLVVPFGEYSFTGAGRKLMTQKI
                     NAVTTNRPLIAFSDNGSSKNGFITGEGIWRWRMEAYRQSGSHQLFNQFVNKMVQYMIT
                     THDKQSLVVDSKRVFNENEPVIIEAEFYDEAFELNNTSELYATITDEKGRILKKLFSR
                     NDNYYTLNLGSLPPGRYSYEAALEFDTKTYSASAEFIVVEINLEQLSSMADHSMLFKL
                     AVENGGKMVYPNQIGTLNGLIRSDKNIVTNMFEQIKLSNLIDLKILFFIILFLLSLEW
                     FLRKFWGVY"
     gene            2673..4073
                     /locus_tag="PLK75_09200"
     CDS             2673..4073
                     /locus_tag="PLK75_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOY39462.1"
                     /translation="MKLRYLDLIEQSFEFPQEEFYVEDGELYFHEIPLMDIVKQYGTP
                     LKFTYLPKISENINRARVWFNVAIAKANYSGKYYQCYCTKSNHFSFVLEEVLKNKVHI
                     ETSSAFDIDIIRSLEESNVVDKTLHIVCNGYKMPVYVEKITELINDGYEWVIPVLDNT
                     GELDKLLEHTEKHLNVGIRIASEEEPKFEFYTSRLGLGYKNIMEYYESNIQNNPRVSL
                     KMLHFFINTGIKDTSYYWNELHKCMQVYCQLKKVCPELDSLNIGGGFPIKNNLAFEYE
                     YEYMAEEIVNQIKLACEENDVPEPDIYTEFGTFTVGESGGALYAIMHQKQQNDREKWN
                     MIDSSFITTLPDTWATSKRYLMLPVNHWEKPYERVFLGGLTCDSDDYYNSEQHVNAIY
                     LPEYDGEETLYIGFFNIGAYQESIGGFGGLQHCLIPCPKHIIINKDEDGKITTKMFSK
                     EQSYKSMLRILGYDYS"
     gene            4128..4991
                     /gene="speB"
                     /locus_tag="PLK75_09205"
     CDS             4128..4991
                     /gene="speB"
                     /locus_tag="PLK75_09205"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOY39463.1"
                     /translation="MSRTFAGIPSEFATFENSKTVLLPVAYDGTSTWGKGADKGPEAF
                     LHAAENMELYDIESASEPFRNGIHLLPLVGGFASPEKMIEKIYVEATKYMAIGKLFSM
                     VGGEHSVSIGALRAANDLYENLTVLQLDAHSDLRPEYDGSTCNHACAMHEASKKSKLI
                     QVGIRSMDIVEVPFLKKENVFFANDIRKKKDWIDQVCSKLTDNTYITIDLDVFDPSVL
                     PSTGTPEPGGMIYYEVLDLLEKVISKTKIIGFDIVELCPNAIDKSSDFLAAKLYYKIL
                     SYIFKKHTHDK"
     gene            4981..5952
                     /locus_tag="PLK75_09210"
     CDS             4981..5952
                     /locus_tag="PLK75_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOY39464.1"
                     /translation="MTSKPITTFIEEHFRHFNAAVVIDAAKAYEALIQKNGKMMITLG
                     GAMSTAELGRSLAEMIRKDKVSIITCTGANLEEDVFNLVAHNHYIRVPNYRDLSPADE
                     QALYDKGMNRVTDTCIPEDVAFRKIEKKMLKIWKDAQAKGERYFPHEFMYKLLLSGVL
                     KSEYQIDPKNSWLLAAAEKNIPVIVPGWEDSTMGNMFAAATIRNDISVNILKTGIEYM
                     VFLADWYRANSKNGIGFFQIGGGIAGDFPICVVPMMIQDLEWENIPFWSYFCQISDST
                     TSYGSYSGAVPNEKITWGKLSPETPKFVIESDATIVAPLIFAHILGW"
     gene            complement(6012..7826)
                     /locus_tag="PLK75_09215"
     CDS             complement(6012..7826)
                     /locus_tag="PLK75_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39465.1"
                     /translation="MKKRNLVLIFIVCLYSITLQAQVQIGSSFQGDNATDRLGFAISM
                     PSATTIGIGAYNNDVSEGYIKIFTLYNNEWVQKGETISSDVVNDNVGGSFSMPDENTI
                     AIGCSGSSANGYNSGKVNVYSWNGSSWIQKGASILGEAAYDGSGISVSMPDENTVAIG
                     ANGNDGDVGIDSDVGHVRVFSWDGSQWIQKGGDIDGENPDDRSGWPVSMPDANTVASG
                     AYYNTGSFSHGGHVRVFEWSNTDWVQKGNDIDGIADGENAGWSISMPNANTVALGAIG
                     YNGSSTAIGQVRIYGWDGSEWVQKGEGIDGEAAYDQSGFSISMPDENAIAIGSIFNQG
                     NGEYSGHVRVYKWVGNSWVKYGSDIDGVAVYNFEGFAVSMPDSKTVATGAPYNNDNGT
                     EAGQVRVFSVCDSYSYYSQEACVSYLSPSGNYSWTSSGLHNDTIPNSLGCDSIISINL
                     TIIGVDISVTNSSPVLIANAEEAQFQWLDCDNNYAVINGEISSIFTATSIGNYAVEVL
                     QNGCIDTSECITVTTISIPELTNEDEVYIYPNPAGERIKIVSLEPIFEIELLNVSGKE
                     LLHLESVNEIDVQSFPPGIYILQFKTDKLIIRRSIVIL"
     gene            8124..9560
                     /gene="pyk"
                     /locus_tag="PLK75_09220"
     CDS             8124..9560
                     /gene="pyk"
                     /locus_tag="PLK75_09220"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOY39466.1"
                     /translation="MFGILDSKTKIIATLGPASQDRESLKKMITEGLDVFRLNFSHGK
                     HEDKKIVIETIRSLEEEMDVNISILADLQGPKIRIGDVENNGVLVEKNDIVEITIKPC
                     IGTSKQLFINYEFFARDVEPGSMVLIDDGKIRLEVLETDRMVCVKTRVVNGGIISSKK
                     GVNLPDTSITMPSLTEKDILDAEFAIKNAVDWIALSFVRKAEDLKDLKKLIEKHNADI
                     DIIAKIEKPEAVSDIDNIVAEACGIMVARGDLGVEVSFSQVPTIQKLIISKCIKAGKP
                     VIIATQMLESMITNFRPTRAEATDVANAVIDHADALMLSGETSVGAFPVDSIKAMQSI
                     IEYTEHNNDIFYLDHKPNESASDFLPDSICYSAGVMAKQTGAKAIVAFTYSGHTAFQI
                     SSYRPKADIYAFTGNKRLLRRLPLLWGVIKTYSIPLYTNIDDAIEHSLRILKSHDVVR
                     PGDLVIHVGSTPMTSKTPTNMLKLTRVV"
     gene            complement(9583..10290)
                     /locus_tag="PLK75_09225"
     CDS             complement(9583..10290)
                     /locus_tag="PLK75_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39467.1"
                     /translation="MKRSFRFLPKNGTKLCETYIGSYTLRIKLRGFFIVFFTVLSSVH
                     CFGQNDSVFKSLQPLFESQEEIQCDSVSSYTVTGKIECKLIFSQRKFDSQGNQSVDIG
                     RIKVAVNDQSILLSEEKITGKKVLQLGYDTCYVREFTQKDNMREYSYLMLFRNKSVAN
                     DWEIEVSPQGSHCIDTDYHWVKISKDKDNFICFSCLYNIKIIELDLNADNQTEIYLIS
                     YIFCEGKAEIYRINNKN"
     gene            11060..>11931
                     /locus_tag="PLK75_09230"
     CDS             11060..>11931
                     /locus_tag="PLK75_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39468.1"
                     /translation="MAKYFYFLLFIICIMQNNTAFSQTQLYGLSYSGGSDQYGTIFHY
                     TPSDETQMVDFSFSSPYPGAAPMYTKLTDVGNGKLYGMTSGGDGINAGGVIFEWDPLT
                     NEYIKKYRWLYTNNPGNTPWGTLTLYNGKLYGTTRWGGNNGDGVIFEYDYVNNIYTKK
                     FDFIEATGAEPKGSLVMVNGKFYGMTSMGGANNLGVIFDWDPATNVYTKRYDLSTTSG
                     TKPDGDLVYYNGLLYGVAYSGGSNNYGTIFSFNPSTGAFTLKLNMASATGGYSTSTMV
                     MRNNKFYGTTRLGGA"
BASE COUNT         3674 a         2280 c         2390 g         3587 t
ORIGIN      
        1 taaaccttaa atcggtgttt aacctgacca aagcagtgca aaaaatcatg cttaagcaga
       61 agaacggatc cataataaac atcagctctg ttgtaggtgt gaacggtaat gcagggcagg
      121 caaattatgc agcttccaaa gccggaatca ttgggtttac caaatccatt gccaaggagt
      181 tgggctccag gaatgtgaga tgcaatgcca ttgctcccgg atttatcgaa acagaaatga
      241 cagcagcatt gccggaggat tacagaaaca cgtggaacga gaaaattgcc ctcagaagag
      301 caggaaaacc agaggatgtt gccgacgttg ttgtattttt ggggtccgat ttgtcgtcgt
      361 atgtaaccgg acaggttatc agtgtgtgtg gaggcatgat ttagccagtg gatgtttttc
      421 aaactaaaag ctttaaaacc tcattgtaaa tgatagctca gtactcgtgg tggtggttgg
      481 ccttatttct agtgttgtca gtaggtatga gcatggcgtt gtactatcgc gacaaggcac
      541 tggcagagct tcaaaaatgg aagatcttac ttctggcact cctgaggttt ctctctgttt
      601 ttctaatatt atgcttgttg tttgctccat tacttaaaac aaagaaaaaa atcattgaaa
      661 aaccaattat tgtattcgca ctcgataatt ccgcatcagt ttcgcgaaac actgattctt
      721 tatattattc ccgacagtac gccaacgaca taaggaacct gatgagtgac ctgagtaccg
      781 attatcaggt gtatcaatac aattttggag aacaaatgcg ccaggactca atcatcgatt
      841 tctccgataa agctactgat atgtctgaaa tttttccaat gattcgtgct gcgcattcag
      901 ggagaaacct tggggcagtt attgttgccg gtgatggcat attcaatgtg ggtgaaaacc
      961 ctgtatactc caccgatata ggcacaactg tttataccat tgcactgggt gatactgtgc
     1021 gctcaaaaga tgtatttata aaacaggtac aacacaataa agttgctttc tacaaaaaca
     1081 gttttccggt acggatcatt gttgaagctg ttcaatgcaa taatgaaaaa attcgcatac
     1141 gtgttcttaa cgtaaatgaa aaactcttcg aaaaagaaat tatcgcgtat gaggacgatt
     1201 gtatcgaaaa aattgaactg gaacttccgg cttcttctaa aggcttacag cagtacgagg
     1261 ttgtaatcga ccgcatcgaa ggagagatca gcaccgaaaa caaccgtcag tcgtttgtag
     1321 tggaagtgat cgataataaa caaaacatac ttattctggg taatagtcct catcccgata
     1381 tcggagctat tgcctatgct ctgaaaagca accccgatta tcagatatcg gtgcaatacg
     1441 ctggcacagt tactgagtca atgaataagt accagcttat tatattacat caggtacctt
     1501 cggttaataa cccagctggt aacattctga atcagatttc acgaaataaa atcccctgta
     1561 tatatatatt aggggcaaaa acatcgcttg atgcactgaa tcttacacaa tcgggtttga
     1621 aaattactgc agccaagtca tctttcgacg aagcaacgcc tttgtttcaa tcttcgttct
     1681 cagcatactc aaatgatcag gaatggatag acattgtgag ctctttgccg ccgttagttg
     1741 tgccatttgg tgaatattca tttaccggtg caggtcgtaa attaatgacg caaaaaataa
     1801 atgcagtcac aacaaaccgt ccgctaatcg cattttctga taatggaagc agtaaaaatg
     1861 gttttattac aggagaaggc atttggcgct ggagaatgga ggcataccga cagtcaggct
     1921 ctcaccagtt gttcaaccaa tttgttaata aaatggttca gtatatgatt accacccacg
     1981 acaaacaaag tttggttgtt gactctaaaa gggtgtttaa cgaaaacgaa ccggtgataa
     2041 ttgaggctga gttctatgat gaagcatttg agctaaataa tacctcggag ttatacgcta
     2101 caattactga cgagaaagga agaatcttga aaaagctgtt tagtcgtaac gataactatt
     2161 acacgctgaa tttgggctct ttgcctccag gaagatattc atatgaagca gctctggagt
     2221 ttgacacaaa gacttacagt gccagtgcag aatttattgt tgtcgaaatc aatctggagc
     2281 agctttcatc aatggccgac cactcgatgc tctttaaact ggctgttgaa aatggcggaa
     2341 aaatggtcta tcccaatcag atcggaacat taaatgggtt aatacggtcc gataaaaata
     2401 ttgttaccaa tatgtttgaa caaatcaaac tcagcaacct aatcgactta aaaatactct
     2461 tctttattat tctgttcttg ctaagtcttg aatggttttt acgcaagttt tggggagtgt
     2521 attaataatg tttttctaac tcattgaaat ataatataat acaattattt tattgaattg
     2581 aaataattgt aaattattta ctcagcttaa ttcgacatat aattaattgc attacatttg
     2641 taaaattaaa ttaatgtaat cgatatagaa ttatgaaact taggtacttg gacttaatag
     2701 aacaatcatt tgaattccct caggaagagt tttacgtgga ggatggtgag ttgtactttc
     2761 acgaaatacc tctaatggat attgttaagc aatatggaac ccctttaaag tttacatacc
     2821 tccctaagat atccgaaaat attaaccgtg cgagagtttg gttcaatgtt gctattgcca
     2881 aggcaaatta ctccgggaaa tattatcagt gttattgcac caagagtaat catttttcgt
     2941 ttgtgcttga agaagtgtta aagaacaaag ttcatatcga aaccagctct gctttcgata
     3001 ttgatattat ccgatcgctg gaggaatcga atgtggttga taaaacactt catattgttt
     3061 gcaatggtta taaaatgcct gtttatgttg aaaagataac tgaattgata aacgatggct
     3121 acgaatgggt aataccggtg ctggataata cgggcgaatt ggataaactg ctagaacata
     3181 ccgaaaagca tttaaatgtc ggcattcgga tagcatcgga ggaggaacct aagtttgaat
     3241 tttataccag caggcttggg ctgggttata agaatataat ggaatattat gagtcgaaca
     3301 ttcagaataa tccaagggtc agcctaaaaa tgctgcactt ctttattaat acagggataa
     3361 aggatacgtc gtactattgg aatgaattgc ataaatgtat gcaggtttat tgtcagctga
     3421 aaaaggtatg ccctgagctt gattctttaa atattggcgg aggatttccg attaagaaca
     3481 atctggcttt cgaatacgaa tacgaataca tggctgaaga gatagtaaat caaattaagt
     3541 tggcttgtga agaaaatgat gtgcctgagc cggatatata taccgaattt ggtacgttta
     3601 ctgtcgggga aagtggaggt gctctatatg caattatgca ccagaagcag cagaatgata
     3661 gggaaaagtg gaacatgatt gatagctctt ttattacaac tctgcccgat acttgggcaa
     3721 ccagcaagcg atatctgatg cttccggtga accactggga gaaaccatat gagcgtgtct
     3781 ttttgggtgg acttacgtgc gacagcgacg attattataa ctccgaacaa cacgttaacg
     3841 ctatatacct tccagaatac gatggtgaag aaaccctgta tatcgggttt ttcaatattg
     3901 gtgcctatca agaatcgatt ggggggtttg gaggcttgca gcactgcctg ataccatgcc
     3961 cgaagcacat tattataaat aaggatgaag atggtaaaat aactaccaag atgttttcaa
     4021 aagagcaaag ctataagtcg atgctgcgca ttcttggtta cgattattct taaaatcatt
     4081 tcatcaattg taataattgt tcatatttgc aaaaataatt gcgttttatg agcaggactt
     4141 ttgcaggaat accatctgag ttcgcgacct tcgaaaacag caaaactgtg ttgctgcctg
     4201 tagcttacga cggcacatct acatggggca aaggagctga caaaggcccg gaggcatttc
     4261 ttcatgctgc agaaaacatg gaactttacg atattgaatc ggcttcggag ccttttcgta
     4321 atgggattca cctgttgcca ctggtaggag gttttgcctc tcccgaaaaa atgatcgaaa
     4381 aaatttacgt tgaagccact aaatatatgg caatcggtaa gttgttcagc atggttggtg
     4441 gcgaacattc tgtaagtatt ggcgctttgc gtgctgcaaa tgatttatat gagaatctga
     4501 cagtattaca actggatgca cacagcgatt tacgacctga atacgatggg agcacttgta
     4561 atcacgcctg tgcaatgcac gaagcgtcga agaaaagcaa gttgattcag gtggggatac
     4621 gaagtatgga tattgtggaa gttccgttcc tgaaaaaaga aaacgtgttt tttgccaatg
     4681 atattcgtaa aaagaaggat tggatcgatc aggtttgcag taaacttacc gataatacct
     4741 acattacaat cgatcttgat gttttcgacc catctgtgtt gccatcaacc ggtacacctg
     4801 aaccaggtgg aatgatttac tacgaagtac tcgacctgct tgaaaaggtg atatccaaaa
     4861 caaagataat tggattcgat attgttgagc tatgccctaa tgcgatagat aaatcctcag
     4921 attttcttgc agcaaaattg tattataaaa ttttgagtta tatatttaaa aaacataccc
     4981 atgacaagta aacctattac tacttttatt gaagagcatt tccgccattt caatgcagcg
     5041 gtggttattg atgcagcaaa agcttatgaa gccctgattc agaaaaacgg aaaaatgatg
     5101 ataacccttg ggggtgcaat gagtacagcc gaattgggtc gttcgcttgc cgaaatgatt
     5161 agaaaggata aagtaagcat tatcacttgc acaggggcta acctcgaaga agatgttttt
     5221 aatctggtag cccacaatca ctatatacgt gttcctaact accgcgacct gtcgcctgcc
     5281 gatgaacagg ccctttacga taaaggtatg aaccgcgtta ccgacacatg tatccccgaa
     5341 gatgtagctt tccgcaagat cgaaaagaaa atgcttaaaa tatggaaaga tgctcaggct
     5401 aaaggcgagc gctatttccc acatgagttc atgtataaac ttttactttc aggtgtgctg
     5461 aaaagcgaat atcagatcga cccgaagaat tcttggttgc tggcagctgc agagaaaaac
     5521 attcctgtaa tagttccggg gtgggaagac agcactatgg gaaatatgtt tgccgctgct
     5581 acaattcgta acgatatttc ggtcaatatt ctgaaaacgg gtatagaata tatggttttt
     5641 cttgccgatt ggtacagagc caattccaag aatggtattg gtttctttca gattggagga
     5701 ggtatcgccg gagattttcc aatttgtgtt gtgccgatga tgattcagga tttggaatgg
     5761 gaaaacattc cgttttggtc atatttctgc cagataagcg attctacaac cagttacgga
     5821 tcgtattccg gagctgttcc caacgaaaaa ataacatggg gaaaactctc acccgaaaca
     5881 ccgaagtttg tcattgaatc ggatgcaact attgttgccc ccctgatttt tgcacacatt
     5941 ctgggctggt aggcgggttg cgaggcagtc aaatcaaaac atttgaagcc taaatcctgt
     6001 ttagaaactg cctataaaat aacaatactt ctcctgatga ttaatttatc agttttaaac
     6061 tgcaggatat aaattccggg aggaaaactc tgtacatcta tttcgttgac gctctccaaa
     6121 tgcaataatt cttttcctga aacatttaat aattcaattt caaaaatcgg ttcaaggctt
     6181 acaattttga tcctttctcc tgcggggttg ggataaatat atacttcatc ttcatttgtg
     6241 agctccggaa ttgatattgt ggttactgta atacattcag aggtatcgat acagccattt
     6301 tgcaatactt caactgcata attcccaata ctggtggctg taaaaatact agagatttct
     6361 ccatttataa ctgcatagtt attatcgcaa tccaaccatt gaaattgagc ttcttcagca
     6421 tttgctatca gcacgggtga tgaatttgtg acagaaatat caaccccaat aatggtaaga
     6481 ttaattgaaa taatgctatc gcatcctaaa gaattgggaa tagtatcatt atgtaatcca
     6541 cttgatgtcc aagagtaatt cccacttggt gatagataac ttacgcatgc ttcttgagaa
     6601 taataactgt aactgtcgca gacagagaaa acacgtacct gacctgcttc cgtgccattg
     6661 tcattgttat agggggctcc cgttgcaact gtttttgaat cgggcatgct tactgcaaaa
     6721 ccctcaaagt tatatacagc tactccgtcg atgtcggaac catatttaac ccacgaatta
     6781 ccaacccatt tatatactct gacatgaccg gaatactctc cgttgccctg attaaatatt
     6841 gatccaatgg caatagcgtt ttcatcaggc atgcttatcg aaaaacctga ttggtcgtaa
     6901 gccgcttccc catcaattcc ttcgcctttt tgcacccatt cgctgccatc ccaaccgtag
     6961 atgcgtacct gaccaatagc ggtactactt ccgttatatc caattgctcc cagtgccact
     7021 gtattggcgt tgggcatact gatcgaccat cctgcatttt ctccgtcggc gataccgtcg
     7081 atatcattcc ctttctgcac ccaatctgta ttgctccatt caaagacacg cacatgacct
     7141 ccatggctaa aactgccggt gttataataa gctcctgatg caacagtatt tgcatcgggc
     7201 atagataccg gccagcccga tctgtcgtcg gggttttctc cgtcgatatc gcctcccttc
     7261 tgaatccatt gagacccatc ccaggaaaat accctgacat ggccaacatc actatcaatt
     7321 ccaacatctc catcgttccc attagcaccg atagcaacgg tgttctcgtc gggcatgctt
     7381 accgaaatac cagacccatc gtaagctgct tcaccaagaa tgctggcacc tttttgaatc
     7441 cagctgcttc cattccatga atacacattt accttgcctg aattgtagcc atttgcggaa
     7501 ctgccactgc aaccaattgc aattgtattt tcatcgggca tgctaaacga tcctccaaca
     7561 ttatcgttta ccacatcact gctaatagtt tctccttttt gaacccattc attattgtac
     7621 agggtaaata tcttgatata tccctccgaa acgtcgttgt tatatgcgcc tattccaatt
     7681 gtggtagcac tgggcataga aatggcaaac ccaagacgat cggttgcatt atctccctga
     7741 aagctactgc caatttgaac ctgagcctga agagtaatgc tatataaaca aacaataaat
     7801 attaaaacaa gatttcgttt tttcattata tggtgttttg gtaatcagtt gaaaaacgta
     7861 caggaagaaa ttgaatattt ttagtaaata ttttcaaatc aacatgttat aatggtatct
     7921 agcacaaaat acgaattttt tttataagtg ctattcctca acatcatttt attattattt
     7981 aaggactttg cattataacc tatttatatg aaatctatta atcaaattga atttaattat
     8041 tgtacgaaat gacttcgatt cattgctatc gtaaaatata atgattattt ttaacacttg
     8101 ttaacccctg aaaatatcaa acaatgtttg gcatccttga ttcaaaaacc aaaattattg
     8161 ctacgctggg tccggcttcg caagaccgtg aatcgttaaa aaaaatgata accgaaggac
     8221 tcgatgtgtt ccgtcttaac ttttcgcatg ggaaacacga agacaagaaa atcgtaatcg
     8281 aaactatccg ctcgctggag gaagaaatgg atgtgaatat ttctattctg gccgacttgc
     8341 agggtccgaa aattagaatt ggagatgtcg aaaacaatgg tgtgctggtt gagaaaaacg
     8401 acattgttga aatcaccatt aaaccttgta tcggaacatc taagcagttg tttatcaatt
     8461 atgagttttt tgcccgcgat gttgagccgg gaagcatggt tctgattgat gacggcaaaa
     8521 tacggctaga agttctcgaa accgacagaa tggtctgcgt taaaaccaga gtggtgaacg
     8581 gtggcatcat cagctcaaaa aaaggagtta atcttcccga cacaagtata acaatgccta
     8641 gcctgaccga gaaagatata ttggatgccg aatttgcaat taaaaatgcg gttgattgga
     8701 ttgccttgtc ttttgtgaga aaagcggagg atttaaagga tctaaaaaag cttattgaaa
     8761 aacataatgc cgatatcgac atcatagcca aaattgaaaa gcctgaagct gtcagcgata
     8821 tcgataatat tgttgccgaa gcctgcggta tcatggttgc acgtggcgat ttgggggtag
     8881 aggtgagttt tagtcaggtc cccaccattc agaagcttat tatcagcaaa tgtattaaag
     8941 caggtaagcc tgtaattatt gccacacaaa tgcttgagag catgattact aatttcagac
     9001 caacccgtgc cgaagcaact gacgttgcca atgcagttat cgatcatgcc gacgcattga
     9061 tgttgagtgg tgaaacatcg gtaggagcct ttccggttga ctcaatcaaa gccatgcaaa
     9121 gcattattga gtacacggag cataacaacg atatttttta tctcgaccac aaaccaaacg
     9181 agagtgcatc tgatttcctg ccggattcaa tttgttattc agccggagta atggcaaaac
     9241 agacgggcgc aaaagccatc gtggcattta cctattctgg gcatacggct tttcagattt
     9301 caagctatcg tccgaaagca gatatttacg cgtttacagg gaacaaacgt ttactgcgcc
     9361 ggctgccgct gctgtgggga gtaattaaaa cctatagcat tccgctctat accaatatcg
     9421 acgatgctat agagcattcg ttgcgaatat tgaaaagtca tgatgtggtt cggccaggcg
     9481 atctggtgat tcatgtaggt tccactccga tgactagcaa aacccccacc aatatgttga
     9541 aactcacacg cgtggtttaa atacccggaa tgtgtccggt tattaatttt tgttgtttat
     9601 acggtaaatt tctgccttgc cttcgcaaaa aatgtagctg attaagtaaa tttcagtttg
     9661 attatctgca tttaaatcaa gttcgataat cttaatattg tacaggcacg aaaaacagat
     9721 gaagttatct ttgtctttgc ttattttaac ccagtgataa tcggtatcga tgcagtgact
     9781 tccttgagga gatacctcaa tttcccagtc atttgcaact gatttatttc taaaaagcat
     9841 taagtatgaa tattctctca tattatcctt ttgggtaaat tctcttacat agcaagtgtc
     9901 gtaacccagc tgaaggactt ttttgccggt tatcttttcc tctgataaca gaatagattg
     9961 gtcgttgact gcaaccttga ttctgccaat atcaacagat tgattgcctt gagaatcaaa
    10021 cttccgctgc gaaaaaatca gtttacattc tatcttccca gttactgtat agctactaac
    10081 tgaatcacac tgaatttcct cctgactctc aaacagaggt tgaagtgatt taaaaactga
    10141 atcgttctgc ccaaaacaat gtactgaaga taacacagta aaaaatacta tgaaaaatcc
    10201 tcttaacttt attcttagag tgtaagatcc gatgtaagtt tcgcaaagtt tagtaccgtt
    10261 tttcggaaga aatcgaaaac ttcttttcat tgaatataat tgtcaaatgc cactttttta
    10321 atcctgtgta aattattaaa taaactcgag gacattaaaa tgtaactcaa tccccatgtt
    10381 gtcgcgaaaa gcagttcatt aattgttttt atttttatca caactacttt tataatttct
    10441 ttctcggata attcgccctt caccaatatt acgcttacag tctgatctga tttttatctt
    10501 tctgattaaa atattacact catcatagaa aaataatatt tcttcagtta tcaaacaatt
    10561 cgtagaagtg tattttattc ttttttttgt aactttcatt ttaccttcat aattttgtaa
    10621 agtatctgtt tcccacttgg tccactggtt ctgaataata ataatatcct gagttttcgc
    10681 agcaatagat atgtaaataa gaaccgcaag aaaacatagt ctctttatga gcttttttgt
    10741 tccaatcatt gaattcataa ccttataaaa aactacccaa ctgaaatata tgaatgaaag
    10801 aagtcatgtt aatatgctaa cctcaaagct aacagttttt tttatattcg ataagaaacc
    10861 cctgttgttt tttttgaagc attgctgtaa agttgcaatt gtgaatacga cagttgagta
    10921 atacattaaa gtttagaaac aaaatagcaa ccaagtatat aataatggtt tccacagaaa
    10981 ttttaaatta agtatttgct atatttgttt aatgtagttt attctttcaa ttgagtatct
    11041 gatattaata actggttata tggcaaaata cttttacttt ctattattta ttatctgcat
    11101 aatgcagaat aatacagctt tctcacagac acagctttat ggcttgtcgt actcaggagg
    11161 gtctgaccaa tatggtacta tttttcatta tactccatcc gacgaaacac agatggtcga
    11221 tttttcattt agttctcctt atccgggtgc agcaccgatg tataccaagc tcaccgatgt
    11281 tggcaatggc aaattgtatg gaatgaccag cggtggtgat gggattaatg cagggggtgt
    11341 tatttttgaa tgggatccac ttacgaacga gtatatcaag aagtataggt ggttatatac
    11401 aaacaacccg ggcaataccc catggggtac acttacgttg tacaatggga aattgtatgg
    11461 tacaacacgg tggggaggaa acaatggcga cggagtgatt tttgaatatg actatgtgaa
    11521 caacatttat actaaaaagt tcgattttat tgaagccacc ggggctgaac caaaaggatc
    11581 tcttgtaatg gttaatggta aattctatgg aatgacttcg atgggtggtg caaataacct
    11641 cggtgttatt tttgattggg atcctgcgac caatgtatat accaagcgat acgaccttag
    11701 cacaacaagt ggcaccaaac ctgacgggga tctggtatat tataatggat tattgtatgg
    11761 agtggcctat tcaggtggca gcaataacta tggtaccatt ttttcattta acccctctac
    11821 cggtgctttt accttaaaat tgaacatggc ttctgcaaca gggggatatt ctaccagcac
    11881 catggtgatg cgtaataaca aattttacgg tacaacccgc cttggtggag c
//
LOCUS       DAOOQP010000087        11928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6658_length_11928_cov_5.580392, whole genome shotgun sequence.
ACCESSION   DAOOQP010000087 DAOOQP010000000
VERSION     DAOOQP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6658_length_11928_cov_5.580392"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLK75_09235"
     CDS             complement(<1..971)
                     /locus_tag="PLK75_09235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOY39469.1"
                     /translation="MRRVVITGMGIYATIGKNLEEVCKSLYEGKSGIGIDPKRYEYGY
                     RSPLTGILDRPELKPFLDRRMRTGLSEEGEYAYMATREALENAKVDMEYLEKNEVGIF
                     YGNDSSAKAVIESHSIAAEKKDTQLMGSGAIFQSMNSTVTMNLSVIYKLKGINMTISA
                     ACASGSHSLGLGYLFIKNGWQDMVICGGAQETNVYSMGSFDALAAFSINVEDPTKASR
                     PFDANRDGLVPSGGAATLILEEYEHAIKRGAPIIAEVTGYGFSSNGGHISQPNNEGSY
                     IAMKRALAEASINPCDVDYINAHATSTPLGDQYEAMAIYKLFGECNTP"
     gene            complement(983..1711)
                     /gene="fabG"
                     /locus_tag="PLK75_09240"
     CDS             complement(983..1711)
                     /gene="fabG"
                     /locus_tag="PLK75_09240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOY39470.1"
                     /translation="MKYALVTGGSRGIGRAVCLQLAGQGYPVIVNYCSNEAAADETCT
                     MIQQAGGIAEKLKFDVALVEEVDTALMKWQENHPDDYIAVLVNNAGVREDTLMMWMQN
                     EQWEKIIGIHLNGFFYVTRRLLKDMLVNRYGRIVNVVSLSGLKGLPGQTNYSAAKAGV
                     IGATKALAQEVGRKGVTVNAVAPGFIATDMTEGLDVKTLKEMVPLNRFGKPEEVADLV
                     GFLASEKAAYITGEVVSINGGLYT"
     gene            complement(1708..3288)
                     /locus_tag="PLK75_09245"
     CDS             complement(1708..3288)
                     /locus_tag="PLK75_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOY39471.1"
                     /translation="MKNGCIKLNAINAIKVDNTLQFFQCVLFEGKKIEIGDEAFKNLE
                     LSFDFLKDFSKDKIIYGINTGFGPMSQYRISDADLKQLQYNLIRSHCSGAGNHIHPMY
                     VKAGMMCRLVNILQAKSGIHPECAVLLKDFINNDIIPVIFEHGGVGASGDLVQLAHLA
                     LALIGEGDVYFQGSIVPASVAMRKCKLKPIGIHIREGLSLINGTSIMTGIAIVNLIYA
                     KRLFNWSVMAGSIINEIVDSFDDGFSKELNAVKLHEGQQQVAKMMRSILAGSKLIKKR
                     QNTLYNRKVTEKIIKEKVQEYYSLRCMPQVLGPVYDTIGFASKIVCSEANSVNDNPIV
                     DMETKNVYHGGNFHGDYISLEMDKLKIAITKMTMLLERQLNFLLNPKLNEKFSPFVNL
                     GVIGLNLGMQGLQFTAVSTTAECQTLSFPNSLHSIPNNNDNQDVVSMGTNSALMTKKV
                     IDNAYEVLSIYLIALLQAVEFMNEKEKMSDTTYNVFNSLRQIVPVFRDDTPKYPEIAK
                     MKQHLMLNDVKIKINGSV"
     gene            complement(3467..4519)
                     /locus_tag="PLK75_09250"
     CDS             complement(3467..4519)
                     /locus_tag="PLK75_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39472.1"
                     /translation="MKQIFILVITILICYSGFTQYYAVPDWFESIPASDDTCLYAVGI
                     SEPRMEDTALALNIATWRALGIASLLQHSHVMYTSDYFETTSEESRTLVMKETVQELA
                     KIESAIAVFQGCYEIVEHEMNDNGEMIVLIRYYRNSSPPNTTVCSEFFRQDFEVSNTL
                     HLEKIRSYSLSVEKTDSSGNIHTVFKAFNLNNSRSYEISYDTIRVIPKGYYYGYYSSM
                     YDSLDISAFTSLASLEKGLWNAFYESFLTSVIRKNKNYRSQFEVVDDSYYTAIDNAPD
                     KANGSLSRQVAKNDISLNLGLMLVDNNNMLHMSLLFPGEMQKEFIMAHKATEDDKKDE
                     KNEKCNWFLKLFKKRK"
     gene            4554..5858
                     /gene="rimO"
                     /locus_tag="PLK75_09255"
     CDS             4554..5858
                     /gene="rimO"
                     /locus_tag="PLK75_09255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOY39473.1"
                     /translation="MKQIPKKIAIISLGCSKNLVDSEQLHFHLQQSGYAVQHNPEKVN
                     AEIVVVNTCGFINDAKEESIDTILGLAEAKKQGNIKKLVVFGCLPQRYMAELKKEIPE
                     VDVFQGNYNLNSLLSEITGISSQHPWFYRSLENPGHYAYLKIAEGCNRKCSFCAIPGI
                     KGKYVSRSIDSIVEEASYLAGGGVKEMLLIAQDTGYYGYDHSKRFMLSTLLNELVPIN
                     GIEWIRLHYLYPGTITRELSLLIKNNDKICRYIDIPVQHISDAVLKRMQRNTKRADIE
                     KAISYLRELIPDIAIRTTLLVGYPGETEKDFDELCRFVESTEFDRLGAFSYSHEENTW
                     GGMHHSDDIPEHIKQQRLETIMSIQQAISLKKNQQKVGKTMKVIFDRKENGQYIGRSE
                     YDSPEIDNEVIVESSRSLVIGNFYQVEITGAGDYEVFGKISK"
     gene            5904..5977
                     /locus_tag="PLK75_09260"
     tRNA            5904..5977
                     /locus_tag="PLK75_09260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5938..5940,aa:Arg,seq:acg)
     gene            complement(6198..6392)
                     /locus_tag="PLK75_09265"
     CDS             complement(6198..6392)
                     /locus_tag="PLK75_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39474.1"
                     /translation="MLSVTARRSTCAEPVEIQIASCSYLTFAKTGSFVFVTVNPEGLP
                     DSYLIGINFSSVFDKKNEDR"
     assembly_gap    6529..6628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6629..6848
                     /locus_tag="PLK75_09270"
     CDS             <6629..6848
                     /locus_tag="PLK75_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39475.1"
                     /translation="STGTSTGTIQAFRRAVAGTSASRLRSTTEDRFALLSVGRVNGSG
                     MVVSSIKKRKPAAKQTGFRLTHNIQHIA"
     gene            complement(6884..7207)
                     /locus_tag="PLK75_09275"
     CDS             complement(6884..7207)
                     /locus_tag="PLK75_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOY39476.1"
                     /translation="MERRHFCNFQVAGFTYYDGPVVFHQLEIGSVVAMRFEPDNRFDP
                     YAIALYFEGYKLGFVPRTCNHQLSKLLEMGYSNCLEARIQSINSQEHPENQLHVIVYL
                     LKSPV"
     gene            complement(7270..8163)
                     /locus_tag="PLK75_09280"
     CDS             complement(7270..8163)
                     /locus_tag="PLK75_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOY39477.1"
                     /translation="MIEQAKFESILRLLVKMSGSFGKTIAELAEDMERSERTIRRYIQ
                     TFRDCGFVVENRNGYFRISEFEKGKNLSDLLHFSSEESELLSRAIHSIDFDGALKESL
                     TKKLYSLYDNNLVPYAIVSAEKSENITALCEAIRQKKQVILHDYSSSNSGRISDRVVE
                     PYDFTTNYTMVCCYEPASNSVKFFKLARIGKVIITDTPWAFENLHAKIFVDVFRISGE
                     NLVRVKLRLSLRAYQLLIEEFPLSENYVTPGDYNSYIFEADVCSYEGVGRFVLGLPDE
                     VSVISPDDFKEFLNEKILKKF"
     gene            complement(8175..9218)
                     /locus_tag="PLK75_09285"
     CDS             complement(8175..9218)
                     /locus_tag="PLK75_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39478.1"
                     /translation="MKKLLVSLSLLCFINVSMFSQNCFLLEKGYINKSVSHNYYSDLT
                     STKGWSKMKPEKKAELINQFNTDVLNGTKAPSSSYFADIYVKDIERGEIEKLTYGLTI
                     NGIEYTFPCLCVDNVHYYYRTVGPVYYVSNGDTIGAGFNGTQAIPNNLKVGDILPSYE
                     DISNLIIGTTSYEDKQRVLDGYREVETIEKNATYLSKQTGRYETGTWKVTKYEEVWKD
                     IDVKVLESTALNSHTIHYVNAIVDRTEEVVLDDKTYTAYVIESEMWIQSGFESTFAAD
                     NAKWQKRRENFQNRLDNQVQKKLIKSKFLNEEGYYVTYLSEWFIPNIGVVKTITYDSE
                     GCISSIGKWDGLK"
     gene            complement(9550..10812)
                     /locus_tag="PLK75_09290"
     CDS             complement(9550..10812)
                     /locus_tag="PLK75_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY39479.1"
                     /translation="MKQISLLFLISVLSISWLSAQNLDYAGKVINDLSSEKYQGRGYF
                     KHGDRKAARYIENEYHKLGLKSWDDYYQNFSFDVNTQHGNHRITIDGRLLVPGVDFVV
                     REYCRSFEGDFNIYRIDTLKPKETTVQELLALDTSNTFVAVEYAYYVRDSLLLTTVNN
                     MKLRNVMLIFDGPLKRYMAASGSTRAARVIWITDNALGKSPARINARFKSKFVKSYKT
                     GNVIGYIEGSQQPDSFFVITAHYDHMGRFGRDVWYPGANDNLSGVAMLLNLAEYYSKP
                     ENKPKCSIAFMAFAAEETGLLGSMNYVQHPFFPLSQVKYVINFDMIADNNPVVYTEVS
                     DNGMRGFEMMKAISSSNMLFEGFDLSELSGNSDHYPFAIEGVPAIFFLDHGDAFKVYH
                     TPLDTLYSESLTLFPSFFKLAQNFIKQY"
     gene            complement(10938..11891)
                     /gene="ftsY"
                     /locus_tag="PLK75_09295"
     CDS             complement(10938..11891)
                     /gene="ftsY"
                     /locus_tag="PLK75_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOY39480.1"
                     /translation="MALFGLFNKEKKEKLDQGLSKTRTGVFDKINRAIFGKSKVDDEV
                     LDQLEEILISSDVGVETTLKIIKRIEERVKRDKILGTSELNNVLRDEIVELLEENHVE
                     NFDSWEFPENSKPYVIMVVGVNGVGKTTTIGKLAYKFNKAGKRVYIGAADTFRAAAIE
                     QIEIWANRVGVPVIKQQMGADPASVAFDTLVSAQKNNADVVIIDTAGRLQTKLNLMNE
                     LSKIKKVMQKVIPDAPHEILLVIDASTGQNAFEQTKQFTLATDVNALAVTKLDGTAKG
                     GVVIGVSDQFSIPVKYIGIGEGIDDLEVFNRREFVESLFKQ"
BASE COUNT         3415 a         2569 c         2286 g         3558 t
ORIGIN      
        1 ggtgtattgc attccccaaa gagtttataa atagccatag cttcatactg atctcccaac
       61 ggtgttgaag tggcatgtgc attgatataa tcaacatcac acggattaat acttgcttcg
      121 gcaagtgcac gtttcattgc aatatatgaa ccttcgttat tgggttgcga aatatgtcca
      181 ccattcgatg aaaaaccgta gcctgtaact tcggcaatta tcggtgcacc tctttttatg
      241 gcgtgttcgt attcctccag tatcaaggtt gctgcaccgc cactgggtac aagaccatca
      301 cgatttgcat caaacggacg cgaggctttg gtagggtctt caacattaat agagaatgct
      361 gccagagcat cgaagctacc catggaatat acattggttt cctgtgctcc gccacatatc
      421 accatatcct gccagccatt tttaataaac agatagccaa gtccgagcga gtgagaacca
      481 ctggcacagg cagcactgat ggtcatgttg atacctttca gtttgtatat caccgagagg
      541 ttcatagtaa ccgttgaatt catcgactga aaaatagccc cggaacccat tagctgagtg
      601 tctttttttt ctgcagcaat gctgtgggat tcgataacgg ctttcgctga cgaatcgttg
      661 ccataaaata ttccaacctc gtttttttcg agatattcca tatcgacttt tgcgttttcg
      721 agtgcttcac gcgttgccat atatgcatat tcaccttctt cggagagccc tgtcctcatt
      781 cggcggtcaa gaaatggctt taattccggt ctatcgagaa taccggtaag cggcgaacga
      841 tacccatatt cataacgttt ggggtctatt ccaattcccg atttaccctc atataacgat
      901 ttacaaacct cttccagatt ttttcctatt gttgcataaa tacccattcc ggtaattacc
      961 actcttctca tagtattact ttttatgtat ataatcctcc attaatcgaa acaacttcgc
     1021 ctgttatgta agcggctttc tctgatgcta aaaagccaac aaggtcggca acttcctcag
     1081 gtttaccaaa gcggttcaat ggaaccattt ccttgagggt cttcacatcg agtccttcgg
     1141 tcatatcggt agcaataaaa ccgggagcaa cagcgttcac tgtaactcct tttctaccca
     1201 cttcctgcgc cagggctttg gtggctccaa taacaccggc tttggctgca gaataattgg
     1261 tttgccctgg caggcctttg agccctgaca atgataccac atttacaatt cttccatagc
     1321 ggttcaccag catatctttc agcaaccggc gcgtaacata gaaaaagccg ttcaggtgta
     1381 tgcctataat tttctcccat tgttcattct gcatccacat catcaatgta tcttcgcgga
     1441 ctccggcatt atttaccaac actgcaatat aatcgtcagg gtgattttcc tgccacttca
     1501 tcaaggctgt atcaacttcc tcaaccaatg caacatcgaa tttcagtttt tcggcaatac
     1561 cgcctgcttg ttgaatcatt gtgcaggttt catcggcagc ggcttcgttg gaacaataat
     1621 ttacaatgac aggatagcct tgccctgcca gttgtaaaca cactgccctg cctatgcctc
     1681 ttgaacctcc tgtaactaat gcgtatttca tactgaaccg ttgattttaa tttttacatc
     1741 gtttaacata agatgctgct tcatttttgc aatttccggg tactttggag tatcatccct
     1801 gaataccgga actatttggc gcagcgaatt aaaaacattg taggtagtat ccgacatttt
     1861 ttccttctcg ttcataaatt caacagcctg caacagtgct attaagtaaa tcgaaagaac
     1921 ctcataggcg ttatcgatca ctttttttgt catcaatgct gaatttgtac ccatgctcac
     1981 cacatcctga ttatcgttat tattcggaat actgtgtagc gagttgggga acgataaagt
     2041 ctggcactca gccgttgtcg aaacggccgt aaactgaagt ccctgcatac caagattcaa
     2101 tcctataacc ccaagattaa cgaatggtga aaatttctca ttcagtttgg ggttcaacag
     2161 aaaattcaac tgtcgttcca acaacatggt catcttggtt atagcaattt tcagtttatc
     2221 catttcaagc gaaatgtaat cgccatgaaa gttgccgcca tggtacacgt ttttggtttc
     2281 catatcaaca atggggttat cgttgactga gttggcttcg gagcacacta ttttagatgc
     2341 aaatccaatg gtgtcgtaaa ccggaccaag tacctgtggc atgcatcgca gcgaatagta
     2401 ttcctgaact ttttctttga ttattttttc ggtaaccttc cggttgtata gtgtattctg
     2461 gcgtttttta atcagtttac tgcctgcaag aatactacgc atcatttttg caacctgctg
     2521 ctgaccttcg tgcaatttaa ctgcattcag ttcttttgaa aatccgtcgt caaatgaatc
     2581 aacgatttcg ttgatgattg agccagccat taccgaccag ttaaacaagc gtttcgcata
     2641 aatcaggttt acaatggcaa tacccgtcat tatcgatgtt ccattaatca gcgataaacc
     2701 ttcacgaata tgaatcccga taggtttcag tttacatttg cgcatagcta ccgaagccgg
     2761 cacaatgctt ccctgaaaat acacatctcc ttcaccgatt aaagcaagtg ccagatgcgc
     2821 cagctgaaca aggtctccgc tagccccaac accaccatgc tcaaaaatta ccggtataat
     2881 atcgttgttg ataaaatcct tgagtaaaac agcacattcg ggatgaattc ccgactttgc
     2941 ttggagaatg tttaccaacc gacacatcat ccctgcttta acatacatcg ggtgaatatg
     3001 gttccctgct cctgaacaat ggctcctgat caggttatat tgcaattgtt ttaaatcagc
     3061 atccgaaatc ctgtattgcg acatcgggcc aaaacccgta ttaataccat aaattatttt
     3121 gtctttagag aaatccttta gaaaatcgaa acttaattcc aggtttttaa atgcctcatc
     3181 gcctatttct atcttcttcc cttcgaacaa cacacattga aaaaactgta atgtattgtc
     3241 aacttttatt gcgttgatag catttaattt aatacatcca tttttcataa taattttatc
     3301 tccccttagc taaaaatgta tcttcaatct ttaaatgttg caaaaataat taatctgact
     3361 gttttagcca aaagcaatac caatttaatt ttatgttatt taatttaagt atgagtttta
     3421 ttagattgta tttcagaaaa atagttaccc aaagaagaat gcagaattat tttcgtttct
     3481 tgaaaagttt caggaaccag ttacattttt cgtttttttc atccttttta tcatcttccg
     3541 tagctttatg ggccatgata aactcttttt gcatttctcc aggaaacaac aacgacatat
     3601 ggagcatgtt attattatcg acaagcatca gacccaaatt cagtgaaata tcgttttttg
     3661 ccacctgacg cgacaaactg ccgttggctt tatcgggagc attgtcgatt gcagtataat
     3721 acgaatcgtc aaccacttcg aactgcgacc ggtagttctt gtttttacgg ataaccgatg
     3781 ttagaaacga ttcgtaaaat gcattccaga gtcctttttc gagcgatgcc aacgatgtaa
     3841 aagcactgat atcgaggcta tcgtacatcg aactgtaata gccatagtaa tatcccttgg
     3901 gaattaccct aattgtatcg tacgatattt cgtaactcct gctgttattc aggttgaagg
     3961 ctttgaaaac ggtatgtata tttccggatg aatcggtttt ttcaacagag agactataac
     4021 tcctgatttt ttcaagatgt agcgtattgc ttacttcgaa atcctgcctg aagaactctg
     4081 aacataccgt tgtattaggc ggagagctgt tcctgtagta ccggatcagt actatcattt
     4141 caccattgtc gttcatttca tgttcaacaa tttcgtaaca tccttgaaaa acggcaatcg
     4201 ctgactcaat tttggcaagt tcctgcaccg tttctttcat aaccagggta cggctttctt
     4261 cggaagttgt ctcaaaataa tcggaggtat acattacatg cgaatgctgc aatagcgaag
     4321 caatacccaa cgccctccat gtggcaatgt tcagcgccag agcagtgtct tccattcttg
     4381 gttccgatat ccccacagcg tataggcaag tatcgtcaga ggcagggatc gactcaaacc
     4441 aatcgggcac tgcatagtat tgagtgaatc cactataaca aatcaatatt gtaataacca
     4501 gaataaaaat ctgcttcatg ctacgtatat ttgcagggtt aaaaataaac ttaatgaagc
     4561 aaattccgaa aaaaattgcg ataataagtc tgggttgttc aaaaaatctt gtcgattccg
     4621 aacaactgca cttccatttg cagcaatcgg gctatgcggt gcagcacaat cccgaaaaag
     4681 ttaatgccga aattgtggtg gtgaatacct gtggttttat aaatgatgca aaagaagaat
     4741 cgatcgacac catactgggt ctggcagaag ccaaaaaaca aggaaatatc aaaaaactgg
     4801 ttgtatttgg ttgtctgccc cagcgatata tggcagaact aaaaaaagaa attcctgaag
     4861 tagatgtatt tcagggaaat tataacctga actcattgtt gtcggaaata acggggattt
     4921 cttcacaaca cccttggttt taccggtcgc tggaaaatcc gggacattat gcttacctga
     4981 aaattgcaga gggctgcaac cgcaaatgtt cgttttgtgc catacctggc attaagggga
     5041 agtacgtatc gagaagtatc gacagcattg ttgaagaagc ttcgtacctt gccggtggtg
     5101 gagtaaaaga gatgttgctg attgcacagg atacggggta ttacggctac gaccattcaa
     5161 aaagattcat gctgtcaacc ttgctcaatg agcttgtccc catcaacggt atagaatgga
     5221 tacgactgca ttatctatat ccaggaacca ttacgcgtga attatcattg cttattaaaa
     5281 ataacgataa aatatgccgc tatattgata taccggttca acacataagc gacgcagtgc
     5341 taaaacgcat gcagcggaat acaaaaaggg ctgatattga aaaggcaatt agctatcttc
     5401 gggaattgat accggatatt gctatcagga ccaccctttt ggttggatat ccgggtgaga
     5461 ccgaaaaaga tttcgacgag ttgtgcaggt ttgtcgaaag tactgagttc gaccgtctag
     5521 gagcattttc atattcgcac gaggaaaaca cgtggggtgg aatgcatcac tccgacgata
     5581 ttccagagca catcaagcaa cagcggctcg aaacaatcat gtccatacag caggcgattt
     5641 cactgaaaaa aaaccaacag aaagtcggga aaaccatgaa ggtaatcttc gaccgaaagg
     5701 aaaacggtca gtatattggt cgatctgaat acgattctcc cgaaattgac aacgaggtca
     5761 ttgttgaaag cagccgaagc cttgttattg gtaatttcta ccaggtggaa attaccggtg
     5821 caggcgatta tgaagttttt ggtaaaattt cgaaataatt gaatgcagtt taattttttg
     5881 ttgtaaattt gcccactgaa aaaggacccg tggctcaatt ggatagagca tctggctacg
     5941 gaccagaagg ttgggggttc gagtccctcc gggttcactg taacaaagac agatgatttc
     6001 atctgttttt tttatttata aaggaggacg agaagtttat cccgatgagg tacgaatcgg
     6061 gaagtccctc cgggttcaca gttacgaaga cgatttttat cgatcttcgt tttttttatc
     6121 gaagacagac gagaagttta tcccgatgag gtacgaatcg ggaagtccct ccgggttcac
     6181 agttacgaag acgattttta tcgatcttcg ttttttttat cgaagacaga cgagaagttt
     6241 atcccgatga ggtacgaatc gggaagtccc tccgggttca cggtaacgaa gacaaaagag
     6301 cccgtctttg cgaatgtgag gtacgaacat gaagcaatct gtatttcaac gggctcagca
     6361 caggtacttc gacgggcggt tacagacagc attacaaatg cttagataaa tgcgccctcg
     6421 ttgcaaacga gggcgagttg gggcttcact gcgttcgcga tgacgtttga gaacccgtct
     6481 ttgcgaatgt gaggtacgaa catgaagcaa tctgtatttc aacgggctnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gcacaggtac ttcgacaggc acaatacaag
     6661 catttcgacg ggcggttgca ggcacttcgg cgtctcgact ccgctcgacg accgaagatc
     6721 gtttcgcttt gctatcggtg gggagggtaa atggctccgg catggtggtt tcgtcgataa
     6781 agaaaagaaa acctgctgca aagcaaacag gttttcgatt gacgcacaac atacaacaca
     6841 ttgcataaac aggatatatg cacaaattgt tcacaaaaag ttgttacacc ggtgacttaa
     6901 gcaaatacac tatcacatgt aattgatttt caggatgttc ctgagagtta atactctgaa
     6961 tacgtgcttc gaggcaattg gaatacccca tctccagcag cttgcttaac tggtgattgc
     7021 aggttctggg tacgaacccc agtttgtatc cttcgaaata caaggcaatg gcataggggt
     7081 cgaaccggtt atcaggttca aaacgcattg ccaccacact tccaatttct aattgatgaa
     7141 aaaccaccgg tccatcgtaa tatgtaaaac cggctacctg aaaattgcaa aaatgtcgtc
     7201 tttccatgat tttatttgta ttagttcggt acaaataaaa gccggcaaat tgtcaactac
     7261 tgacaatcgc taaaatttct ttaaaatttt ttcgttgaga aactctttaa aatcatccgg
     7321 agaaatcacg cttacttcat cgggcaaacc cagtacaaac ctgccaacgc cttcgtagct
     7381 gcacacatcg gcttcaaaaa tataggaatt gtaatcaccg ggggtaacat agttttcgga
     7441 aagaggaaac tcttcgatga gcaattggta tgcccttaat gacaaccgca gtttaacacg
     7501 gacaagattt tcgccggaaa tacggaatac gtcaacaaaa atctttgcat gaagattctc
     7561 gaaagcccat ggagtatcgg taataatgac tttcccgata cgtgccagct tgaagaattt
     7621 taccgaatta cttgccggtt cgtaacaaca caccatggtg taatttgtgg tgaaatcgta
     7681 aggctccacc accctgtcgc tgattctgcc actattcgac gaggaataat cgtgcagaat
     7741 tacctgcttt ttctgcctga tggcttcgca gagggcagtg atattttctg atttttcggc
     7801 actaacaatg gcatatggaa ccaggttatt atcgtagagc gagtacagtt tttttgtaag
     7861 actttcttta agagcaccgt cgaaatcgat ggagtgtatt gccctggaga gcaattcaga
     7921 ttcttcggat gaaaagtgga gtaagtccga taagttcttc cccttttcga attccgaaat
     7981 tctgaaatac ccatttcggt tctccacaac aaatccgcaa tcgcggaaag tttggatata
     8041 tcggcgaatc gtccgctccg accgttccat gtcttcagcc agttctgcga tagtttttcc
     8101 gaacgacccg ctcattttca ccaacaaccg gagaatgctt tcaaattttg cctgttctat
     8161 caaaataaat tatattattt taaaccgtcc catttaccaa tactactgat gcatccctcg
     8221 gaatcgtagg ttattgtttt aacaacgcca atatttggaa taaaccattc agaaagataa
     8281 gtcacataat atccttcctc gttaaggaac ttcgatttaa taagtttctt ctgaacctga
     8341 ttatccagtc tgttctgaaa gttctcacgg cgtttttgcc attttgcatt atcagcggca
     8401 aatgtgcttt caaagcccga ttgaatccac atttcgcttt cgattacata ggcggtatag
     8461 gttttatcat ccagtacaac ttcttcggtg cggtcaacta ttgcgtttac ataatgaatc
     8521 gtgtggctgt tcaaagctgt cgattctagt acctttacat cgatatcttt ccaaacctcc
     8581 tcatatttcg tcaccttcca agtgcccgtt tcatacctcc ctgtttgctt gcttaaatat
     8641 gtggcatttt tctcaatggt ttccacttca cgatatccgt cgagaacccg ttgcttgtct
     8701 tcgtaagaag tggtgccaat aatcaaatta gagatatctt cgtaactggg aaggatatcg
     8761 cctactttaa gattgttggg aatagcctgc gtcccattaa atcctgcccc aatggtatcg
     8821 ccattactca cataataaac cggtccgaca gtcctataat aataatggac attatctacg
     8881 cacaagcaag gaaatgtgta ttcaattcca ttgatcgtta atccgtaggt cagtttttca
     8941 atttcgcctc tttcaatgtc tttaacataa atatcggcaa aatacgaaga tgatggagct
     9001 tttgttccgt ttaatacatc ggtgttaaat tgattaataa gctcagcctt cttttcgggc
     9061 ttcattttac tccagccctt ggtagaagtc aaatctgaat aataattgtg agataccgat
     9121 ttattgatat accccttttc gagcaaaaaa caattctgcg aaaacatgga tacattaata
     9181 aaacacagca aggataaaga aacaagcaac tttttcatag taattggttt tacatttcct
     9241 actcatacgc ttttcggtaa ccgcgtgcat taagtatttg gcataagcca aattttaata
     9301 caataatggt tctactacca ttttagtggg taaattcaca aaaagcatgg ctatcttgct
     9361 atatatcatc tatttatcat tgtatgcgcg taatattagg gaatctgttc gtaactattt
     9421 gcttaacaga ttgatatgtc gtaaatttca tcttctttcc actaaatttg cagtagaacc
     9481 acaataataa acaaatataa gtacgatatt tcaattaaac aatacgaaaa acagaaaaac
     9541 aatcgcaatt tagtactgtt taataaaatt ttgcgccagt ttaaaaaatg aaggaaataa
     9601 tgtaaggctt tcgctgtata gtgtgtccag aggagtatga tatactttaa atgcatcacc
     9661 atgatcgaga aagaatattg ctggaacacc ttcgattgca aatgggtagt ggtcgctatt
     9721 accactcagt tccgataaat caaatccctc aaaaagcatg ttgctgctac ttattgcctt
     9781 catcatttcg aagccgcgca tcccattgtc gctaacctcg gtgtaaacaa cagggttgtt
     9841 atccgctatc atatcgaaat tgattacata ttttacttgc gacaatggaa aaaatggatg
     9901 ttgaacataa ttcattgacc caagaagccc tgtttcttca gcggcaaacg ccataaatgc
     9961 aatggagcat ttcggtttgt tttcaggttt cgaataatac tctgccagat tcagaagcat
    10021 agccacacca ctcaagttat cgtttgcacc agggtaccaa acatcgcgtc cgaacctgcc
    10081 catgtggtcg taatgggcgg tgatcacaaa aaaactatcc ggctgctgcg aaccctcaat
    10141 gtaaccgatg acattaccgg ttttgtagct tttcacgaat ttgcttttaa aacgagcatt
    10201 tattcgcgct ggagattttc ccaacgcatt gtcggtgatc cagataaccc gggcagctct
    10261 ggttgatcca gatgcagcca tatagcgttt cagcggacca tcgaaaataa gcataacatt
    10321 tcgcagcttc atgttattca cagtagtaag caacaggctg tccctgacat agtatgcgta
    10381 ttccactgca acaaaagtgt ttgatgtatc aagagccaaa agttcctgaa ctgttgtttc
    10441 ttttggtttt agggtatcga tacggtaaat attaaaatca ccttcgaacg aacgacaata
    10501 ctcacggaca acgaaatcga cccctggaac caataatcgt ccatcgatgg taatgcgatg
    10561 attgccatgt tgtgtattga cgtcaaagga aaaattctga taatagtcgt cccagctttt
    10621 taaccccagt ttatgatact cgttttcgat atatcgggca gctttacggt cgccatgttt
    10681 aaaataaccc cgtccctgat atttttccga cgacaggtcg ttaattactt tgcccgcata
    10741 atcgaggttt tgcgcactca accatgaaat agacaatacc gaaattaaaa ataacagaga
    10801 gatttgtttc ataaaaaatc ttttcttcaa atataggttc agtggaagtc agattttctt
    10861 aataaaaata taaaacgatt gtagcaagta tatattcaat ctgaattgct tgcagataat
    10921 aagaacctta aatagcccta ctgtttgaac aggctctcga caaactctct gcggttgaat
    10981 acttccagat cgtcgatacc ttctccgata ccaatatatt ttacaggaat gctgaactgg
    11041 tcggaaacac cgataaccac tccacctttg gcagttccat cgagttttgt aacagctagg
    11101 gcattcacat ccgtagcaag agtgaattgt tttgtttgct caaaagcatt ctgaccggtg
    11161 gaggcatcaa ttaccagcag tatttcgtga ggtgcatcgg gaataacttt ttgcatcact
    11221 ttttttattt tcgaaagctc attcatcaga ttcagttttg tctgcaatct accggccgta
    11281 tcgattatca ccacatcggc attatttttt tgagctgaaa ccagtgtatc gaatgccacc
    11341 gaagcaggat cggctcccat ttgctgttta atcacaggca ctcctaccct gtttgcccag
    11401 atttcaatct gttcaattgc tgcagcacga aatgtatcag cagcaccaat atacactcgt
    11461 ttaccggctt tattaaactt gtatgcaagt tttccgatag tagtagtttt accaactcca
    11521 tttaccccga caaccataat tacatagggt tttgagtttt cggggaattc ccacgaatca
    11581 aaattctcaa cgtggttttc ctcaaggagt tccacgattt cgtcgcggag tacattattc
    11641 agttcggagg tacccaaaat tttgtcgcgt ttaacacgtt cttcgatacg tttaataatt
    11701 ttaagtgtgg tttctacacc aacatccgaa gatatcagta tctcttccag ttgatcaagc
    11761 acttcatcgt caactttgct ttttccaaaa atagccctgt tgattttatc gaaaacacct
    11821 gtccgtgttt ttgacaagcc ctggtcgagt ttttcttttt tttctttatt gaataatcca
    11881 aataatgcca tggaatttgc tttgttaaga acagccacaa agatacat
//
LOCUS       DAOOQP010000088        11780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6765_length_11780_cov_6.640000, whole genome shotgun sequence.
ACCESSION   DAOOQP010000088 DAOOQP010000000
VERSION     DAOOQP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_11780_cov_6.640000"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1137)
                     /locus_tag="PLK75_09300"
     CDS             complement(121..1137)
                     /locus_tag="PLK75_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY39481.1"
                     /translation="MIEKFSTTCCLLLLGLLMQGQDIHFSQFLSSPLNLNPALCGSYE
                     GDLRLCLNHKGQWQSFANAYSTFSASFDANLKAPFVRKSRTGLGILLNSDKSGDGNLS
                     TNQVKLNFSWNQPLTRDSSIYLAAGVNAGFTNHGVDVTLLHFGSQYIDGQFNPDAPTN
                     EYFNATSISYFDYSAGINLGYIANANVLYHIGFSLQHINEPEKSFNQNEHIVLQRKFV
                     YHAGCEWNVKDNLYIDPFLLFMHQGPYIESDLGAIVRIAYNPLGLKYVFTGISMRTLD
                     AGILMLGANYQNIRLTLSYDINLSKLSNISYGRGGIELSLIYIYQKPRPFKPPYYRKC
                     PEFM"
     gene            1230..2513
                     /locus_tag="PLK75_09305"
     CDS             1230..2513
                     /locus_tag="PLK75_09305"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39482.1"
                     /translation="MLRGRKILFFFVVVLMFVPLLQEYFHIFKVKPLLGSYNLAAKPE
                     IGFKQWFSGSCQDSLNDYYNQHFTLRPVFVRVNNQLDYSLYRKSNAKKVVIGKSGVLF
                     ENNYLLAWQGADFIGEKPIDSIMELASSAQNLLAQKGVDMLVVLAPGKASFFPEYIPA
                     PYNKKVSEFTNYELISKKCQEKGIHYIDLNCWFSEIKSASPYPLYPKCGIHWSEYGMF
                     LASDTIIKTIESIRKVNLPDMYIKNIEITKQQLGLDYDIGNTLNLLFPINAYPMAKLD
                     IGYSNTTNYKPDVLVIGDSYYWNIYLSSIPGNVFRSKDFWYYNLNAYNDGTNIMAATT
                     NTLDYKSEILKRDVIIILQTDGGLNNFGFNFFTDVINTLNNPGFNDRVEQYRQAILKD
                     DAWKKSVEEKARQRNIPFEEMLLLDAKYMAEHEAQ"
     gene            complement(2516..3400)
                     /locus_tag="PLK75_09310"
     CDS             complement(2516..3400)
                     /locus_tag="PLK75_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39483.1"
                     /translation="MKTYMYYAVVLLVLASCSSTPLSKKEKELNEKITNLETENKSLK
                     KSDADKDKAIKDFVGTLNEISANLEVIKQKENVISLSAKDVELNSTEIEKISSDILLI
                     NDLMDKNRRTIEQLHNDLENSIIYSEELKQIIDNFSIVINQQEFEIRRLQEQLMKVSK
                     SFVLLQQDIDSLNAANAEQKQQLDEHETAMNTGYYISGYANYLTENEIVFVTGGIFGI
                     GSVFILSEDFDKKIFKSISISETKKIPLNCSSAQLLSVHPSNSYKFDGPSDHVNNLII
                     TRPREFWSNTKYLVILKK"
     gene            complement(3459..3800)
                     /locus_tag="PLK75_09315"
     CDS             complement(3459..3800)
                     /locus_tag="PLK75_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY39484.1"
                     /translation="MKSDITIPLLKEYLQFTIEVIDFCDYLTKQKKDVIANKLLDAAI
                     GVSSTLQSCKLVERESDCAANLKKVKKNMEEVNYWLGQCHKSENYHHNQELLETGRAL
                     DKKLIDLLTKK"
     gene            complement(3951..4625)
                     /gene="rsmI"
                     /locus_tag="PLK75_09320"
     CDS             complement(3951..4625)
                     /gene="rsmI"
                     /locus_tag="PLK75_09320"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOY39485.1"
                     /translation="MSKLIIVPTPLGNLGDITFRALEVLRTADVILAEDTRKTAILLH
                     HFGIKTKMLSHHKFNEHQFAEVICNRILQGETMALVSDAGTPSVSDPGYLLVRTCIRS
                     AVEVECLPGPTALIPALVVSGLPSDRFVFEGFLPVKKGRQKRLSELANESRTIIFYES
                     PHRIAKTLEQLSQHFGSERLASVSREISKIHEETLRGSLNELLSHFAKINSKGEFVIV
                     VAGHDQ"
     gene            complement(4618..5892)
                     /locus_tag="PLK75_09325"
     CDS             complement(4618..5892)
                     /locus_tag="PLK75_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY39486.1"
                     /translation="MIRLIFFIFFFAFLYLVATGQDTSYVRRNISELSGEEYHGRGYV
                     KKGVNKAAHYLASEMEEIGLQCLGKDYYQSLTYPVNTFPGNITVRVDGKLLQTGTDYF
                     IGAACPQVDREYKVVVFDSVTLSDTIRFFAKAGMMRLKDALVVIDFSYLQASDVRWYY
                     VNLMKSNFIQAAGYIEISNGNLAWATRNAQSDFPVLKIKKNAFSAETETVYIRVKPKL
                     IKNYKTENIVGYIPGTSDEYIVIGAHYDHLGMMGKNVFIPGANDNASGTVMVLDLARY
                     YKQHPGKYNMVFILFTGEEAGLMGSEYFTENPLIPLEKTKIMLNFDMVGTGEKGAAIV
                     NGEAEGYEKIAAKFIEINNRNNYLKDIKLRGLSKNSDHYPFHEAGVKAVFFYTMGADT
                     FYHSPGDVVDNLSLQAYIPLFKLVTDFISEYE"
     gene            complement(5889..6695)
                     /locus_tag="PLK75_09330"
     CDS             complement(5889..6695)
                     /locus_tag="PLK75_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39487.1"
                     /translation="MKNLSLKTEEILASLKRIEANGMQQIDIDLCLQQIRELYSEVLQ
                     LPDLEKLIADKPAVEISATQPQSYFEFAPPTEITLIDEKVDPIAPSEPVQTTSEALPE
                     TNTLSEKPITAQEFKPEVVDTVVEPVKTETKPAEKKATDKKITQPSLFNNGTESETKT
                     IGEQLGEKKTSLNEVLGLKTQQQDVVSRIQAKPITDIKAAIGIGDRFLFIRELFDGDN
                     DLYNKTLEYLNALSEINEANEYLKANFTWNNENSTVDHFMKIVQRKFIKQ"
     gene            6771..7349
                     /locus_tag="PLK75_09335"
     CDS             6771..7349
                     /locus_tag="PLK75_09335"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOY39488.1"
                     /translation="MFLERPGYEHNIKGWIEVIAGSMFSGKTEELLRRLRRAKYAKQK
                     IEIYKPSVDTRYSETNVVSHDENSFPSTPVSVSSNILLLAGEVDVIGIDEAQFFDNGL
                     ADVCNQLANRGIRVIVAGLDMDYLGRPFGPMPALMAIAEYVTKVHAICVQCGDLAQHS
                     YRVSASDNLVLLGEKDSYLPLCRKCYNESIKK"
     gene            7353..9797
                     /locus_tag="PLK75_09340"
     CDS             7353..9797
                     /locus_tag="PLK75_09340"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOY39489.1"
                     /translation="MTPYTLLDLNIAVQGQAFGNTAIVVESLSTNSRTVFNPEGVVFF
                     AIPGKNHDGHIYIEALIEKGVKAFIVSDPTCIREIKDCGFILTDNVVLALQKIAAFHR
                     SKFNIPLVGITGSNGKTIVKEWLNYLLSSQLRVTRSPKSFNSQIGVPLSVWQLSEKSD
                     IGIFEAGISMPDEMDYLEKIIHPDVGVFTNLGNAHQKNFTSLHDKASEKAKLFRNAGT
                     IVWCADDPIIDGIIPRDSNRKLLSWSFGNKGAIRAMVSQNSECCSVIEITSKSETYQF
                     KINYTDKASIENAITCFVCCMALHCDLHCLIPLFSTLPIVEMRLEMIDGRNSCRIIND
                     SYSLDINSFEIALDNLVSHAANKDTILIISDFQQSHLPESELYAAVSRMITAHRITRI
                     IAIGKIISKNKNLFPPETAFFEETKQFIDSSLWMTFRNSEILIKGARSFCFENIVTLL
                     QKQTHETVFEINLNAVESNLNYFRQISGKKVKTMVMVKAFSYGTGYFEIAGFLQYLKV
                     DYLAVAYIDEGIDLRRGGISMPIMVMNPRREAYDKMFEFSLEPEIYSLGMFEDCIHAI
                     KTHGLRKYPVHLKLDTGMHRLGFSENDIEPLQFLIEGNREYLKIQSVFTHLVGADDPK
                     HDEFTHKQVAGFSSMASRLADDGCIQHVLNSSGIIRFPQYCFDMVRIGIGLYGFAAGT
                     SNLINTGTLKTTISQIRQVEQGETIGYNRHGKVMRKSLIATIPVGYADGFNRHLGNGK
                     WQVIINNQKAPVIGDVCMDMTMIDVTGLNVKQGDEVIVFGSEMPVTEMAKVLGTIPYE
                     ILTGISQRVKRVYYQE"
     gene            9960..10715
                     /locus_tag="PLK75_09345"
     CDS             9960..10715
                     /locus_tag="PLK75_09345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY39490.1"
                     /translation="MKFTAVIITFNEERNIERCIQSLVPLTDDIVVVDSFSEDATVDI
                     CHKYGARVFQRSFAGYSDQKNWANSQANYDYIFSIDADEVVSAELQQSIQNIQTPLAE
                     TAFIVNRLTNYCGKWIRHGGWYPDKKTRLWNKNNAIWVGLIHETVSFSKPVATIPLKG
                     DLQHYSYISIDDHHNQAMKFAKLSAVQMFENCKKTTLLTILVAPAFRFLKTYLFKGGF
                     LDGYYGFVIAKISARATFYKHLWLYKKKCDKNL"
     gene            10793..>11780
                     /locus_tag="PLK75_09350"
     CDS             10793..>11780
                     /locus_tag="PLK75_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39491.1"
                     /translation="MRKITLMLSLLAFVSFAFAQVSGTYRATSDINKQVVTSQAVKAP
                     GDILWEVTFDEATPVWTFGQVSGTKTWNVTDTTPDYGYTNANYQGGTVPVPPLWLYMG
                     WQYVHDWSDSGEKFAWVDGISDLLGAGGNTPQVCNTYIQFDGIDMTGVTAPKLSFYQD
                     YKGFNGDHCYIDFSTNGGSTWETLEINQGVAGNAYAEPTFEVFVGDYIGNQGNVSLRF
                     RWETTGTTAATYGYGWQIDDVRIIENALNDLKLVDARMNFFEYIDYTVAGQGDYFHSS
                     SHYGMIPNEEFFSEFANMWFNGMFENKGMNSVAPEFNVIVLNPQDEEIFNETV"
BASE COUNT         3491 a         2369 c         2350 g         3570 t
ORIGIN      
        1 agaagttctt atcctgaaat gcaatatcgg ctgacttaat ggtactaata aggttttgcg
       61 atgagagagc tgttgcaaaa caaaggaaga aaagcaatat gtataatttc tttatttgca
      121 ttacataaat tccgggcatt tacggtaata cgggggttta aaaggtcgtg gtttctgata
      181 aatatatatc agcgaaagtt caatacctcc cctcccatag ctgatattcg acaatttcga
      241 aagattaata tcgtagctca gagtaagcct gatattctgg taattggcac ctaacatcag
      301 tattcctgca tctaaagtgc gcatactgat accagtaaat acatacttta aacccagagg
      361 attataggca attcgaacta ttgcacccaa atccgattca atgtacggac cctgatgcat
      421 aaagagcaga aatggatcta tgtacaaatt gtctttaaca ttccattcac aaccggcatg
      481 atacacgaat ttcctctgta acacaatatg ttcgttctga ttgaacgatt tctccggctc
      541 attaatatgt tgtaacgaaa atccaatgtg atacaaaaca tttgcattgg caatataccc
      601 aaggttaatt ccggctgaat aatcgaaata gctgatactg gtcgcattga aatactcgtt
      661 cgtaggtgca tcagggttaa attgtccgtc gatatactga ctgccaaaat gaagcagggt
      721 tacatctact ccatggttcg taaaaccggc attaacaccg gcagccaaat aaatcgaaga
      781 atctcgggtt agtggctgat tccaagaaaa attgagtttc acctgatttg tactcaaatt
      841 accatcaccc gacttatcag agttcagcag gattcccaac ccggttcttg atttgcgaac
      901 aaacggagcc ttcaggttgg catcgaacga agcagagaat gtggaatatg cattcgcaaa
      961 cgattgccat tgtcctttat gattaaggca caggcgaagg tcaccttcat atgaaccaca
     1021 taaggccgga ttaagattta atgggcttga aagaaactgg ctgaaatgaa tgtcctgacc
     1081 ctgcatgagc aacccaagca acaataaaca gcaagtcgtg gaaaatttct caatcacaga
     1141 aatcaatcat tcaggttata aagtcaaatt taataaaata tatttcattc aaatatcgaa
     1201 aattaaaaat acttagttaa tttgcgttta tgcttagagg tcgtaaaata ctgttttttt
     1261 tcgttgtagt tttaatgttc gttccattac tgcaggagta ttttcacatt ttcaaggtaa
     1321 aacccctgct gggatcgtac aacctggcag ccaaaccgga aattggattc aagcagtggt
     1381 tcagcggaag ttgtcaggac agcctgaatg actattataa ccagcacttt acgttgcggc
     1441 cggtttttgt aagggttaac aatcagttgg actatagcct ttatcgaaaa agtaatgcta
     1501 aaaaagttgt gattggcaaa tcgggagtcc ttttcgaaaa taattatttg ctggcatggc
     1561 agggtgccga ttttatcgga gaaaagccaa ttgacagcat catggaatta gcatcaagtg
     1621 cacagaatct gctggcccaa aaaggtgtgg atatgctggt tgtacttgcc ccggggaaag
     1681 ccagtttttt cccggagtat atacctgctc catataataa gaaggtatca gaatttacca
     1741 attacgagct aatttcaaaa aaatgtcagg aaaaagggat acattacatc gatttaaact
     1801 gttggttttc tgaaattaaa tcagcctccc catacccgtt gtaccccaaa tgcggaattc
     1861 actggagcga atacgggatg tttctcgcat cggatactat tattaaaaca attgagtcga
     1921 tacgcaaggt taatttgcct gacatgtata ttaaaaacat tgagattaca aagcaacagt
     1981 tgggcttgga ttatgatatc ggtaacactc tcaatctgct tttcccgatt aatgcttatc
     2041 ctatggcaaa actcgacatt ggctattcaa atacaacaaa ttacaagccc gatgtactag
     2101 ttattggcga cagttattat tggaatattt acctgagttc aattccagga aatgtattca
     2161 ggagcaaaga tttctggtat tataacctga atgcttacaa tgatggcacc aatatcatgg
     2221 ctgctacaac caacactctc gattataaaa gcgagattct gaaacgggat gtgattataa
     2281 ttttacaaac tgatggtggt ttgaataatt ttggcttcaa cttctttact gatgtaataa
     2341 acactctgaa taacccggga ttcaacgata gggttgagca gtacagacag gctattctga
     2401 aagacgatgc atggaaaaaa tccgttgaag aaaaagcacg tcaacgcaat atacctttcg
     2461 aagagatgct actgcttgat gcaaaataca tggctgagca cgaagcacag taaagttatt
     2521 ttttaagaat tacgaggtat ttagtattac tccagaattc acgcggtcgt gtgattatga
     2581 ggttattcac atgatcggaa ggaccatcaa atttatacga gtttgaaggg tgtaccgaaa
     2641 gtagctgtgc gctactgcaa ttcaacggaa ttttctttgt ttcggaaatg gaaatacttt
     2701 taaatatttt cttatcgaag tcttcactta atataaatac actgccaatt ccgaaaatac
     2761 ctccggtaac aaacactatt tcattttcgg taaggtagtt agcatagccg gaaatataat
     2821 aaccggtatt cattgccgtt tcgtgttcat cgagttgttg tttttgttcc gcattagctg
     2881 cattcagcga gtcgatatcc tgttgcaata gcacaaaaga tttgctcacc ttcattaatt
     2941 gctcttgcaa tcgcctgatt tcaaactcct gttgatttat cactatcgaa aaattatcga
     3001 taatctgctt caattcttcc gaataaataa ttgaattctc aaggtcgttg tgcaattgtt
     3061 caatagttcg cctgttttta tccatcaggt cgttaatcag caatatatca ctgctgattt
     3121 tttcaatttc ggtgctgtta agctcaacat cttttgccga taacgatatt acattttctt
     3181 tctgctttat tacttctagg ttggcactga tttcgttcag ggttccaaca aaatccttga
     3241 ttgccttatc tttgtcggca tccgactttt tcagcgattt attttcagtt tcaagattgg
     3301 tgattttttc attaagttct ttttctttct tcgacagggg tgtactactg catgaagcca
     3361 gcaccaacaa tacaactgca taatacatgt aggttttcat aatctttcaa taaaacatga
     3421 taacgtacaa agtaacaggt tttgtacaaa tgttgaggtt atttttttgt taataagtca
     3481 ataagctttt tatcaagtgc ccgtcctgtt tccagcaact cttgattatg gtgatagttt
     3541 tccgacttat ggcattgacc cagccagtaa tttacttctt ccatattttt cttcactttt
     3601 ttaaggttcg cggcacagtc cgactccctt tcaacaagtt tacacgactg aagtgtgcta
     3661 ctcacaccaa tcgcagcatc gagcagtttg ttggcaataa catctttctt ttgtttggta
     3721 agataatcgc agaaatcaat tacttcgatg gtaaactgaa ggtattcctt taacagaggt
     3781 atagtaatat ccgatttcat gataataggg tttgatgaac aaatgtaata ataattttca
     3841 attgagcctg aatattttgc aaaattttca tagctcccca tcacaacagc aaacactagg
     3901 ggattgcagg cgtattggtt gactcacaag ttattacaaa aattgccagt ctattgatca
     3961 tggccggcaa caacaattac aaattcacct tttgagttaa ttttagcgaa atgcgacaga
     4021 agttcgttca aactgccccg aagggtttct tcatggattt tacttatttc gcggcttacc
     4081 gatgcaagcc gttcggagcc aaaatgctgc gataattgct ccagtgtttt tgcaatacga
     4141 tgtggcgatt cataaaaaat tatagtacgc gattcatttg ccagttcgct taatcgcttt
     4201 tgcctgcctt ttttaacagg aagaaaacct tcgaaaacaa accggtcgga aggtaaaccc
     4261 gaaacaacaa gagccggtat aagggccgtt ggcccgggaa ggcactctac ctccactgct
     4321 gaacggatgc aggtacgcac aagtagatat cccgggtcgg aaaccgatgg tgttccggcg
     4381 tcactaacca aagccatggt ttctccctgt aagattctgt tgcaaatgac ttcggcgaac
     4441 tggtgttcgt taaacttatg atgagaaagc attttagttt taattccaaa atgatgcaac
     4501 agaatcgccg ttttccgggt atcttcggca agaattacat cggctgtgcg aagcacttcc
     4561 aatgccctga aagtgatatc cccaagatta ccgagcggtg ttggaacgat aatgagttta
     4621 ctcatattct gaaatgaaat cagttacaag tttaaacaaa ggtatataag cttgaaggct
     4681 aagattgtca actacatcgc cgggactatg gtaaaaggta tctgcaccca tagtataaaa
     4741 gaataccgcc ttcactcctg cttcatgaaa aggataatga tcgctgttct tcgacagacc
     4801 cctcaattta atatctttca ggtaattgtt gcgattattg atctctatga attttgccgc
     4861 aattttttca taaccctctg cctcaccgtt tacaattgca gccccctttt caccggttcc
     4921 aaccatgtcg aagttaagca ttatcttggt tttctccaat ggaattaatg ggttttcggt
     4981 aaaatattcc gaacccatca acccggcttc ttcgccagtg aaaaggatga aaaccatatt
     5041 gtattttccc ggatgttgtt tgtagtagcg tgcaagatcc aacaccatga ctgtaccgct
     5101 ggcattatcg ttagcacccg gaataaatac attttttccc atcattccca gatggtcgta
     5161 atgtgcacca ataactatgt attcatcgga agttcccgga atatatccta cgatattttc
     5221 cgttttatag tttttaatca gtttgggttt tacccgtata tatacggtct ctgtctctgc
     5281 agagaaagcg tttttcttga ttttaagaac cggaaaatct gattgtgcat tacgggttgc
     5341 ccatgcaagg ttgccattgc tgatttcgat ataacccgcg gcttgtatga aattactttt
     5401 cattaaattc acgtaatacc agcgaacatc ggatgcctgc aggtaagaga agtcaatcac
     5461 cacaagcgca tctttaagcc tcatcatccc agctttggca aaaaaacgaa ttgtgtcgga
     5521 gagggttacc gagtcaaata caacaacctt gtattccctg tcaacctgag gacatgccgc
     5581 accaataaag tagtctgtac ccgtctgaag cagtttgcca tcgaccctaa cagttatatt
     5641 gcccgggaac gtatttaccg gataggtcaa cgattggtaa tagtcctttc ccaaacactg
     5701 caatccaatt tcttccattt cactggcaag gtaatgggca gctttattca ctcctttttt
     5761 cacataacct ctgccgtgat attcttcacc gctcaactct gaaatattcc tgcgaacata
     5821 agaagtatcc tgccccgttg caaccaaata taaaaatgca aaaaagaata taaaaaatat
     5881 taaccgaatc attgtttaat aaattttctc tgaactattt tcataaagtg atcaaccgta
     5941 ctgttttcat tattccatgt aaaatttgct ttcaggtact cattggcttc gttgatttcg
     6001 ctcaatgcgt ttaggtactc cagagtctta ttatacaaat cgttatcgcc atcgaacaac
     6061 tccctgataa acaaaaaacg atcgccaatt ccaatagcag ccttaatatc ggtaatgggc
     6121 tttgcctgaa tgcgtgaaac cacatcctgc tgctgggttt tcagtccaag cacttcgttc
     6181 agcgatgttt tcttctctcc cagctgctcg ccgatggttt ttgtttccga ttcggttccg
     6241 ttattaaata aactcggttg cgtaattttt ttatctgttg ctttcttttc tgcaggtttt
     6301 gtttcagttt ttacaggttc aacaactgta tcaacaactt ctggtttaaa ctcctgtgct
     6361 gtaattggtt tttcggaaag tgtgttagtt tcgggtagtg cctcagaagt agtttgaact
     6421 ggctcagatg gagcaatagg gtcaactttt tcatcgataa gtgtaatttc ggtcggtggc
     6481 gcgaattcaa aatagctctg gggctgtgtt gcagaaattt caacggcggg tttgtctgct
     6541 atgagtttct ccagatcagg cagttgaagc acttcggaat acaattcgcg tatctgctgc
     6601 aaacaaaggt caatatcgat ctgttgcatt ccgtttgctt ctatcctttt tagcgatgcc
     6661 aaaatttctt cggtttttaa acttaaattt ttcatgtgat tcttccttac gagttttttt
     6721 ctacatttgt gtattctgaa tgacaaaaat aattatttta ttgaaccaat atgtttttag
     6781 agcgtccggg atatgaacat aatattaaag gctggattga agtgattgcc ggatcgatgt
     6841 tttcgggaaa aactgaagag ttgttgcgac ggttacgcag ggcaaaatat gccaaacaga
     6901 agatcgaaat ttacaagccc agtgttgata cacgctattc cgaaaccaat gttgtgtcgc
     6961 acgatgaaaa ctcttttccc tctacaccgg tgagtgtttc ttcaaacata ttattgcttg
     7021 ccggcgaagt tgatgttatc ggtattgacg aagcccaatt tttcgacaac ggactcgccg
     7081 atgtatgcaa tcaactggca aacagaggca tacgcgtaat tgtcgcaggg ctggatatgg
     7141 actacctcgg ccgacctttt ggtccgatgc ctgcacttat ggccatagcc gaatatgtaa
     7201 ctaaagtaca cgccatttgc gtacaatgtg gcgacttagc ccagcattcc tacagggtta
     7261 gcgcctccga taacctcgtt ctccttgggg aaaaagattc gtatttgccc ctttgccgca
     7321 aatgctacaa cgaatcgata aaaaaataat caatgactcc ctatacacta ttggatctga
     7381 atattgccgt acagggtcaa gcctttggca atacagccat tgttgtcgaa agcctgtcga
     7441 caaacagccg cactgttttt aatccggaag gggttgtgtt ttttgccatt ccaggtaaaa
     7501 atcatgatgg gcacatatat attgaagctc tcatcgaaaa aggtgtaaag gcatttattg
     7561 tttccgaccc aacctgtatc cgcgaaataa aggattgcgg ttttatactt accgacaatg
     7621 tagttcttgc tttacagaaa attgccgcat ttcaccgcag taaattcaac atacctttgg
     7681 taggtattac cggcagtaac gggaaaacca ttgttaaaga atggctgaat tatttacttt
     7741 cgtcgcaatt gagagttacc cgcagtccga aaagtttcaa ttctcaaatt ggcgttccgc
     7801 tatcggtgtg gcaattgagc gaaaaaagcg atataggcat ttttgaagcc ggcatttcga
     7861 tgccagatga aatggattat ctcgaaaaaa tcattcatcc agatgtcgga gtttttacaa
     7921 acctggggaa tgctcatcaa aagaatttca cgtcgttaca tgataaggct tccgaaaaag
     7981 caaaactgtt ccgtaacgca ggcacaatag tctggtgtgc cgacgaccca ataatcgatg
     8041 ggattattcc cagagattca aaccgaaagt tgctttcatg gtcgttcggc aacaaggggg
     8101 ctattagggc aatggttagc caaaattccg agtgctgctc ggttatcgaa attacaagca
     8161 agtcagaaac ctatcagttt aaaattaatt acaccgacaa agcttccatc gaaaatgcaa
     8221 ttacctgttt tgtatgttgc atggcattgc attgcgatct ccactgtctg attccgcttt
     8281 tttcaaccct gccaattgtt gaaatgcgcc ttgagatgat cgacggtaga aactcctgcc
     8341 gtataattaa cgattcctac agtttagaca taaattcttt tgagatagca ctcgacaatc
     8401 tggtttcgca tgctgcaaac aaagatacca ttcttatcat cagcgacttt cagcaaagcc
     8461 acttacctga atcagagtta tacgctgctg tcagccgcat gattacagct cacagaatta
     8521 cacgcatcat tgccattggg aaaataattt cgaaaaataa aaacctgttt ccgcccgaga
     8581 ctgcattctt cgaagaaaca aagcaattta tcgacagttc gctttggatg acttttcgca
     8641 attcggagat cctaattaaa ggcgcaagga gtttttgttt tgaaaacatt gttactctat
     8701 tgcagaagca aactcacgag actgttttcg agataaacct gaacgcagtt gaatcgaacc
     8761 tgaattactt ccggcaaatt tccggaaaaa aagttaaaac catggttatg gtcaaggctt
     8821 tttcgtatgg aaccgggtat ttcgaaattg cagggtttct ccagtatctg aaagtggatt
     8881 accttgctgt tgcatacatc gacgaaggca tcgatcttcg acgtggagga ataagtatgc
     8941 cgattatggt gatgaaccct cgccgcgagg cttacgacaa aatgtttgag ttttctttgg
     9001 agcctgaaat atattcgctc ggaatgttcg aagattgtat tcatgcaata aagacacatg
     9061 gactacgaaa atatcccgtt cacctgaagc tcgacacagg aatgcacaga ctcgggttct
     9121 ctgaaaacga tattgagcca ttgcaattcc taattgaagg caaccgtgaa tatctgaaga
     9181 ttcaatctgt attcacccat ctggttggtg ccgacgatcc gaaacatgat gaatttacgc
     9241 ataaacaggt tgccgggttt tcttcaatgg ccagccggtt ggcagatgat gggtgcatac
     9301 agcatgttct gaacagctct gggattatac gatttcctca gtattgtttc gacatggtac
     9361 gtatagggat tggtttgtat ggttttgctg ccggcacaag caacctgata aatactggga
     9421 cactaaaaac aactatatcg cagatacggc aagtggaaca aggggaaaca atagggtata
     9481 accgccatgg taaagtaatg cgcaaatcgt tgattgctac catcccggtt gggtacgccg
     9541 atggctttaa ccggcatctg gggaacggaa aatggcaggt tataataaat aaccaaaaag
     9601 caccggtaat cggcgacgtt tgcatggaca tgacaatgat tgacgttaca ggactcaacg
     9661 taaaacaggg tgatgaggtg atcgtgtttg ggtcagaaat gcctgttacc gaaatggcaa
     9721 aggtccttgg aacaatacct tacgagattc ttaccggtat ttcgcagcgg gtaaaaagag
     9781 tatattatca agagtagaaa tcaccgtatt tcgaaaatta gttggtagaa ttccacacga
     9841 ttgttgcctt tcgccttatt aattaactca ctttaacagg caaagtgcct attattttaa
     9901 ggtttttaat gttttttttt tagttttgat gattgatacg tattataatt ccttcagtaa
     9961 tgaaatttac cgcagtaatt atcacattca acgaggaacg aaatattgaa agatgtattc
    10021 agtcgcttgt tcctctaacc gatgacattg tggtagttga ttctttttcg gaagatgcaa
    10081 ctgtagacat ttgccataaa tatggagcca gagtatttca gagatctttc gcaggttatt
    10141 ccgaccaaaa aaactgggca aacagtcagg caaactatga ttacattttt tcgattgatg
    10201 cggatgaagt tgtttctgca gagcttcaac aaagcataca aaacattcag acaccccttg
    10261 ccgaaacagc gtttattgtg aatcgtctca caaactattg tggcaaatgg atacgccatg
    10321 gcggttggta tcccgacaaa aaaacaaggc tttggaataa aaataatgcg atatgggttg
    10381 gtttgataca tgagactgtt tctttcagta aacctgttgc aaccatacct cttaaaggcg
    10441 atttgcagca ttattcttac atttcgatag atgatcacca caatcaggca atgaaatttg
    10501 caaaactaag tgctgtccag atgtttgaga attgcaaaaa aacaaccctt ttaacaatac
    10561 tcgttgcgcc cgcatttcgt tttttaaaaa catacttatt caaaggcgga tttctagatg
    10621 ggtattacgg ttttgtaatt gcaaaaattt cagcgcgtgc aaccttttac aaacatttgt
    10681 ggttatataa aaaaaagtgt gacaaaaatt tataacgata atttttaaaa atatttattt
    10741 tatatttgca tgtttttaat tgaaaactaa ttattaattt aaatttatag ctatgagaaa
    10801 aattacatta atgttatcat tgcttgcttt tgtttctttt gcttttgcac aggtttctgg
    10861 aacctaccgt gcaaccagcg atattaacaa acaagttgtt acgagtcagg cagtaaaagc
    10921 ccccggtgat attctttggg aagttacttt cgacgaagcc actccggtat ggactttcgg
    10981 tcaggtttcc gggacaaaaa cctggaacgt aactgacaca actcccgatt atggatatac
    11041 caatgccaac tatcagggtg gtactgttcc tgttccacca ctatggttgt acatgggctg
    11101 gcaatatgtt cacgactggt cagattcagg tgaaaaattt gcatgggttg acggtatttc
    11161 cgatttactt ggtgccggtg gcaatactcc tcaggtttgc aacacatata ttcaattcga
    11221 tggtattgat atgaccggtg taacagctcc caaattatca ttctatcagg attataaagg
    11281 tttcaacggc gaccattgct acatcgactt ctccaccaat ggcggttcta cctgggaaac
    11341 cctcgaaatc aatcaaggtg ttgcagggaa tgcgtatgca gaacctactt ttgaagtatt
    11401 tgtaggcgac tatattggca atcaaggcaa tgtttctctc cgtttccgtt gggaaaccac
    11461 cggaacaact gctgcaacat acggatacgg ctggcagatt gatgacgtaa gaatcattga
    11521 aaatgcactg aacgacctca aactggttga tgcccgtatg aacttcttcg aatatatcga
    11581 ttatactgtt gccggacaag gtgattattt ccactcaagt tcacattatg gcatgatccc
    11641 taatgaagaa ttcttcagcg aatttgcgaa catgtggttt aacggaatgt tcgaaaacaa
    11701 aggtatgaac tcggtagctc ccgaattcaa cgtaatagta ttgaacccac aggatgaaga
    11761 aatcttcaac gaaacagtta
//
LOCUS       DAOOQP010000089        11693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6846_length_11693_cov_5.049493, whole genome shotgun sequence.
ACCESSION   DAOOQP010000089 DAOOQP010000000
VERSION     DAOOQP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6846_length_11693_cov_5.049493"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1874)
                     /locus_tag="PLK75_09355"
     CDS             complement(9..1874)
                     /locus_tag="PLK75_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOY39492.1"
                     /translation="MVLKFFSTQILMFIVFALHAQMILEYNTALSSGTTIMLPLYGAV
                     DVSVDWGDGNTETHNIEGNKSHTYSANGTYIVTISGLLTHFGTGNYGFAEKLTRIIDF
                     GDIGLTDLSFSCSNATNLTEVPEQIPATVTNLSSMFYNANSFNMDIGSWDVSNVTDMS
                     SMFSDADSFDQPIGNWDVSNVTNMSQMFKYATNFNQPIGNWDVGNVTDMSEMFSYTSY
                     FNQDINNWDVSSVTDMYQMFRSVLVFNQPIGSWNVSSVTNMRQMFQDSHFFNQELGDW
                     DVSNVNNMFAMFTNAVAFNQPIGNWNVGNVTDMSSMFSYAHDFNQDIGNWDVSNVTNM
                     NSMFKSADAFDNNIGNWDVSNVTDMGLMLGGVTLSVDNYDALLIGWASLDLHPNISFS
                     GGYSKYSCAAIAAHDILTNNPNNWIITDGGNIEDEVDPTISCPSDFTIIADDESQTYT
                     VEGNELDPLLYSDNCPGFFVINDYNSMSSLYGTVFNLGTTCVLWTIEDGSGAQTSCSF
                     DVTIEAFVGLADNDALCVFSVYPNPAHEQIIVDVGTNYSAMEEITLEIVNTLNQVVFT
                     TQINSHQTVLDFSDWAGNGIYLVRLINAKRNTLNTKKIVIINYSPFLRPLFCILN"
     gene            complement(1966..2694)
                     /locus_tag="PLK75_09360"
     CDS             complement(1966..2694)
                     /locus_tag="PLK75_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY39493.1"
                     /translation="MINCIAVDDERLALELLEDNIRKVPFLNLIATCSSAKHAIEVVQ
                     SEKIDLVFLDIEMPEINGLQLVKGLVHKPAVIFITAYEKYAIDGYDLDVVDYLLKPVM
                     PERFLKAATKAFEYIRFLHQDKAEEQGNNNIAFIFVRSERKILKLNTSDILYIEGLKD
                     YAKIFTGTKPILSLVTLKHLEDILPEKEFVRIHRSFIIALSKINYISRSSVYIGEKSI
                     PVSNFYRDSFFKRISDNTGKSIIF"
     gene            complement(2691..3758)
                     /locus_tag="PLK75_09365"
     CDS             complement(2691..3758)
                     /locus_tag="PLK75_09365"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY39494.1"
                     /translation="MAKVFTSKYWLLLIHILGWAGYFLFQILTENHFGPNGSLTHDTV
                     WWIIQMAIILFFYVNYLALIPFLLQRKRYFLYALSIIAILVLTLFGLDFFIHSVSDKL
                     PDSAMNPANIPHHHGPPPDDKGIFKGLSVFIMVYVISTGLKMTSEWFRGERQKQVLAN
                     EKLTAEITALKAQINPHFIFNVLNNICSLARKKSDETETYIIKLSQLLRYNLYEDKGG
                     KILLEKEISFLTQYIDLQKMRLGDEVSVILDIELKDSSMLIEPMLLFPFIENAFKHGI
                     SYSEHSEIKIRMKAEGKILFFSVTNPIVTQHSNLHTEPSSGIGLTNVNRRLELLYPQK
                     HNILVNQENGLFTVSLEIDLS"
     gene            4003..4587
                     /gene="ruvA"
                     /locus_tag="PLK75_09370"
     CDS             4003..4587
                     /gene="ruvA"
                     /locus_tag="PLK75_09370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOY39495.1"
                     /translation="MYEYIKGKLTELNAAYAVVESGGIGYSIQISLNTFSKLTLNDNA
                     LLYLHFIVREDVQQLFGFADKTERNVFKHLIAVNGIGPNTARMILSSLGPEELMQAIA
                     TKNISVLKSIKGIGQKSAERMVIELKDKLVTSDADFSHNFNNVSNSLYDDALQALLML
                     GFNRASIDKVLREIMKSSTESSVEQIVKRALKEL"
     gene            4589..>11693
                     /gene="sprA"
                     /locus_tag="PLK75_09375"
     CDS             4589..>11693
                     /gene="sprA"
                     /locus_tag="PLK75_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOY39496.1"
                     /translation="MIAKIVKYTLGIASLLTLIAVIFPSNATLIDYSHQAPGINDDTI
                     PGDDTIPDVILPYPYSDNDGNPFTQDDNSPLYGSDPENISNDIEYDPETGDYIFRRKV
                     GGRDIETPFNASFNDYLDYDFDNSMKSYWRQRARSESFESYSSLIPKLQVSGEVFDRI
                     FGGNTIDIRPQGAAELSFGLNISYVDNPTYPENIRRSVTFDFDEKIQMNVVGQIGDKM
                     KLNVQYDTESAFDFENSVKIEYTGYDDEIIQKIEAGNVSLPLSGTLISGSQSLFGFKT
                     ELKFGRLNITTLFSQQKGEASTIEVQGGAQTKEFEIYADKYEANKHYFLSHYFEENYN
                     RFLSSLPLITSGINITKVEVWVTNRQGNFETSRNIVAFTDLGENNPDNVESSYTLSGS
                     FSNVPYPSDSANILAIIESLIPEVRNINSAGTSLLAAGFEGGVDYEKIESARMLSTSE
                     YTVNTQLGYISLNSTLTSDQVLAVAFEYTIGGKVFRVGEFSNSAVTAPDALILKLIKG
                     TSFTPQMKVWDLMMKNIYSIGAYQLSNDDFYLDVYYKNDKTGTEINFLPAGAIDSVRL
                     LTVLNLDDLNSQLDPYADGIFDFINGVTVNASNGRIIFPVTQPFGTYLRQAITGGNPS
                     LNEVADQYVFQELYDSTQSTARQIAEKNKFFIAGKYKSSSGSDISLNAINIPQGSVKV
                     TAGAQVLTENVDYTVDYNLGRVKIINQGILESGTSIQISIESNSMFNIQTKTLVGTHL
                     NYEISKDFNIGATILNLTERPLTQKVSIGDEPISNTIWGVNTSYRTNAGWLTKAIDFI
                     PFIETKEMSTITFSGEFAQLIPGHSKAIDKEGNAYIDDFEGAETNIDIKSYSAWTIAS
                     TPNDPNMFPEHSGASPLDVGYNRARLAWYVIDPLFYRSNSPVDVELQSSHMVREVYES
                     EIFPYKESVTGIPTNLSVMNLAYYPTEKGPYNYSINGMNADGSLSDPYSRWAGIMRKL
                     ETNDFEEANIEYIEFWMMDPFVEDSLNPGGDIYFNLGDVSEDVLKDGRKSFEHGLATP
                     ANQNPVDTTIWGIVPLIQSLVTAFDNDAVSRQYQDVGLDGLSDEQERGFFSSASGWST
                     YLDQIAALYGTGSMAYQKALNDPSNDNYHYYQGSDFDSAGYGITRRYYNFNGLEGNSP
                     SSDQSGEDYSTSASASPNIEDINRDNTLNENETYFQYRVSIRPEDLVVGKNFITDKIE
                     YNAKFANNERSRVTWYQFKIPVHSYDRRVGTIQDFKSIRFIRMFMKNFNQNTIVRLAT
                     LDLVRGEWRKYNDSFMQPGEYNPDELVETPFVVTAVNIEENGNKTPVNYILPPDINRQ
                     IDPTNPQLRQLNEQSMVLKVIDLQDGDGRAVYKNLDMDMRKYKKLKMFIHAEAIVGET
                     NLYDDDLCVFIRLGSDYKNNYYEYEIPLKVTAPGHYEGSEEDDPDRLIVWPDANNLSL
                     EFELLQDVKQMRNDEMRMLGTGVTLTRLYTIMDGGNKVSIMGNPNLANVRTVMIGVRN
                     PKRAGATSDDDGMPKSGEIWVNELRLTDFDEKGGWAANARFTAKLADFGSLTIAGQTS
                     KPGFGSINEKLTERQKEEINRYDVSSNLELGKFFPEKLNVRIPLYMGYSENVKNPEYN
                     PLDPDIPLSMALTDPTKTPEEIKEIKYKSQDYTSLKSLNFNNVKVNKTQGKARIYDLA
                     NWSASYGFNESFHRDINTVFNTNKTISGALSYNYNATPKNYEPFKNAKFLNKPAFRLI
                     KDFNLYLLPSQLAFRTNLNRQYSENQLRNVYNESVALPLSVSKDFIWQRQYDLKYNIS
                     KGLKIDFSATNSARIDEPEGRIYRGDPEYESKMDTIYNNLKNFGRNTQYHHTIDVTYT
                     IPINKIPLFNWVTANARYSANYDWVSGPITADTINIGNTVQNGNAISANVQFSLLNLY
                     NKINYLKEVNQKYRGNKSKNAKPKKETVKFEVADVKLKANKKKTINHKLKTSDVSLVV
                     VSGDGKTIKGTTEIIDDNKVIFTPEEDADNAKITVNGSRELKDGVAKIIFDNTLVLLM
                     SVKSVSIGYTQNNGTLLPGYMPNTQIVGLSKYTPDPEMFGVQPSVTAPTIPFILGWQD
                     RDFGEWAYEHNMLTKDTTLAQAFAFMHSTNWNVRASIEPVRDLRIDLTLTHSFSENVS
                     EYFRYNNQISDFDRLNPLTTGSYSMSTISWGTAFEITGRKGDYSSDNFQRFSDYRKEI
                     AVRLASQRPDYNPGNVDEFGYPIGFNQFSQDVLLTSFMAAYTGKDPQKCTLETFPNVF
                     NALPNWRITYDGLGKINALKKIFRTVNISHVYRSTYNIGSYTTRLSDQYFENDGFNYI
                     REVLGQNQEGAITYGNFYSKYEVNSVNISEQFSPLISFDMTMVNSFIAKVEVKKNRNL
                     SMSF"
BASE COUNT         3713 a         2435 c         2311 g         3234 t
ORIGIN      
        1 taaagatttc aattaagaat acaaaaaagt ggtctaagga atggggagta gtttattatt
       61 acgattttct tagtattcag cgtattcctt tttgcattaa tcaaacgaac taaatatatt
      121 ccgttgccgg cccagtccga aaagtcaagt acagtttgat gtgaatttat ttgcgttgta
      181 aaaacaactt ggtttaatgt atttactatt tctaatgtga tttcctccat tgcactataa
      241 tttgtgccaa catcgactat aatttgttca tgtgccggat ttggatacac cgagaatacg
      301 cacaatgcat cgttatctgc taatcctaca aaagcttcaa tagttacgtc gaaactacaa
      361 gaagtttgtg cgcccgaacc atcttctatt gtccagagta cacatgttgt tcctaaatta
      421 aatacagttc catataaact gctcatactg ttataatcgt ttataacgaa aaaccccgga
      481 caattatcac tataaagcaa tggatctaac tcgttaccct caacagtata agtttgactt
      541 tcatcatcag caattatggt aaaatcagac ggacatgaga ttgttggatc tacttcatct
      601 tcaatattac cgccatctgt aataatccag ttatttggat tattggttaa aatatcgtgc
      661 gctgctattg cggcacaact atatttacta taaccaccag aaaaactaat attggggtgt
      721 aaatctaagc ttgcccaacc aataagcaat gcgtcgtaat tatcaactga aagagtcacg
      781 ccacccaaca ttaagcccat atcagttacg ttgctaacat cccaattgcc gatgttatta
      841 tcgaatgcat cagctgattt aaacatcgaa ttcatgtttg ttacattact tacatcccag
      901 tttccaatat cctgattaaa atcgtgagcg tatgaaaaca tcgaactcat atctgtaaca
      961 ttgccaacat tccagttgcc aatgggttga ttaaacgcta cggcattagt aaacatagca
     1021 aacatattat tcacattact aacatcccaa tctccaagtt cctgattaaa aaagtgtgaa
     1081 tcttgaaaca tctgacgcat atttgtaaca ctgcttacat tccagctacc aatcggttgg
     1141 ttgaatacca atacactacg aaacatctga tacatgtcgg taacgctact aacgtcccag
     1201 ttgttaatat cttgattgaa atatgatgta taactgaaca tttcactcat atccgttaca
     1261 ttaccaacat cccaatttcc aataggctga ttgaagtttg tagcatattt aaacatctga
     1321 ctcatatttg taacattact tacatcccag ttcccgattg gttggtcaaa tgaatcagca
     1381 tcactaaaca ttgaactcat atcagtcaca ttactcacat cccagctgcc aatgtccata
     1441 ttaaatgaat ttgcattata aaacatcgaa ctcaaatttg ttaccgttgc cgggatttgc
     1501 tcaggaacct cagtcaaatt tgtcgcatta ctacacgaaa acgataaatc agttagtccg
     1561 atatctccaa aatcaataat tcttgtcaat ttttcagcaa atccataatt gccggtgcca
     1621 aaatgagtga gtaagccgct aatagttacg atgtaagtgc catttgcact ataggtatgg
     1681 cttttattac cttcaatgtt atgcgtttct gtattcccat cgccccagtc aacgctaaca
     1741 tcaaccgcac cgtacaaagg taacatgatg gttgtgcccg aagataaagc ggtattgtat
     1801 tccagaatca tctgtgcatg caaagcaaat acaataaaca taagtatctg ggtagagaaa
     1861 aatttaagta ccataatata ctatccagca ttattaaaca tctgccaaat tatacaattt
     1921 tttcgataat ttataactaa ttgtagatga attttgaata aaaaatcaaa atataataga
     1981 tttaccggta ttgtcgctga ttcttttaaa aaaactatcg cggtagaagt tgctcacagg
     2041 aatgcttttt tcaccgatat aaacactgct gcgacttatg tagttgattt ttgaaagcgc
     2101 aatgatgaag gaacggtgga ttcttacaaa ttccttttca ggcagaatat cttccaggtg
     2161 cttcaacgta acaagcgata gaatgggctt agtgcctgta aatattttag catagtcttt
     2221 taagccttca atgtacagta tatcggaggt gtttaatttt aatatttttc gttctgagcg
     2281 aacaaatata aaggctatgt tattattacc ttgttcttca gctttatctt gatgtaaaaa
     2341 acgaatgtac tcaaaggctt ttgtagcagc ttttagaaag cgctcgggca ttacgggttt
     2401 cagtaggtag tcaaccacat ccaaatcgta accatcaatt gcatattttt cgtatgcagt
     2461 aataaaaatt acagccggct tgtgtaccaa tccttttaca agctgaagtc catttatttc
     2521 aggcatttca atatcaagga aaaccagatc tattttttcg gattgtacca cttcaatagc
     2581 atgttttgcg gaactgcacg ttgcaatcag gttcagaaac ggaaccttcc ggatattatc
     2641 ctcaagtaac tccagagcaa gcctttcgtc gtcgacagca atacaattta tcatgataaa
     2701 tcaatttcaa gacttaccgt gaatagcccg ttttcctgat tgaccagaat attgtgcttt
     2761 tgtggataaa gcaactcaag tcggcggtta acatttgtaa gaccaatgcc cgaggaaggt
     2821 tcagtgtgaa gattgctatg ctgcgtaacg ataggattcg ttaccgaaaa aaatagaatt
     2881 ttaccctcag ctttcattct gattttaatt tcggagtgtt ccgagtagct gatgccatgc
     2941 ttgaatgcgt tctcgataaa tgggaaaaga agcataggtt caataagcat actcgaatct
     3001 ttcaattcaa tatcaagaat taccgaaact tcatcgccca gtcgcatttt ttgcaggtcg
     3061 atatattgtg tgagaaatga gatttctttt tcaagcagaa ttttgccccc tttatcttcg
     3121 tacaaattat aacgtagcaa ttgagaaagc ttaatgatgt aagtttcggt ttcatcagac
     3181 tttttacgtg caagtgaaca gatattgttc aaaacattaa atataaaatg aggattaatc
     3241 tgagctttta atgcagtgat ttcagcggtg agcttttcgt tggcgagcac ttgcttctgg
     3301 cgctctcccc gaaaccattc actggtcatt ttcaaaccgg ttgaaataac atataccatt
     3361 ataaaaactg atagtccttt gaaaatgcct ttgtcatcgg gtggcggacc atgatgatgc
     3421 gggatgttgg cggggttcat agcagagtca gggagcttat ccgatactga atgaataaag
     3481 aaatctaaac caaacagtgt aagtacaaga attgcaatta tactcaacgc atacaaaaag
     3541 taccttttcc tttgcagaag gaaaggaatt aatgcaaggt agttgacata aaaaaacaaa
     3601 atgatagcca tttgaataat ccaccaaacc gtatcatgtg tcaggctacc attgggcccg
     3661 aaatgatttt cggtaagtat ctgaaacagg aagtaccctg cccaccccaa tatgtgtatc
     3721 agtagaagcc agtattttga agtgaacact ttagccatca gtacaatttt tgcaaaatta
     3781 cgaaaaaaaa atcccttacc tgcaaataat cgatataatg attattgccc gtgccggaaa
     3841 gcattttaaa accgacagat tatttttttt gaatcgttcg acctgttaac aatacttacg
     3901 atgcttaatt ataaaaacta atattttatt tagaatggca gaagctatta attgtaacat
     3961 ttttttattt ttgcaaatct tagtcaaagc atatttcgac atatgtacga atatataaaa
     4021 ggaaaactta cggaactgaa tgcagcttat gcagttgttg aaagcggagg aattgggtac
     4081 tcgattcaaa tatcgttgaa cactttttca aaacttactc tcaacgataa cgccttgcta
     4141 tacctgcatt ttattgttcg cgaagatgtg cagcagttgt ttgggtttgc cgataaaaca
     4201 gagagaaatg tatttaaaca tctcatcgca gttaatggaa ttggcccgaa cacggcaagg
     4261 atgattcttt catctctggg acctgaggaa ttgatgcaag ccattgccac aaagaatatt
     4321 tcggtgctga aatcaataaa aggaataggc cagaaatcgg cagaaagaat ggttattgag
     4381 ctaaaagata aattggtgac gtcggatgca gatttttcac ataattttaa caatgtaagc
     4441 aatagtttat acgatgatgc gttacaagca ttattgatgt taggttttaa tcgtgcatcg
     4501 atagataaag tgcttaggga aattatgaaa agcagtaccg aaagttctgt ggaacagatt
     4561 gttaagcgtg cattaaaaga gctgtagttt gattgcgaaa attgtaaaat acaccctggg
     4621 aattgcctcc ttgctaacat taattgcagt gatattccca tcaaatgcaa ccctgatcga
     4681 ttactctcat caggcccccg gaataaatga tgatactatt cccggtgacg atactattcc
     4741 cgatgtaata ttaccgtatc cgtattccga taacgacggt aatccattta ctcaggacga
     4801 taattcacct ttatacggaa gcgatccgga aaatatttcc aacgacattg aatacgatcc
     4861 cgaaacaggt gattatattt tccgccgtaa agtgggaggg cgcgatatag agactccatt
     4921 caacgcttcg ttcaacgatt atctcgatta cgatttcgac aattcgatga aaagctactg
     4981 gagacagcgt gcccgtagcg aatcttttga gtcgtattct tcattgatac ctaaattgca
     5041 ggtcagcggt gaggtattcg accgtatttt cggcggcaac actattgata tacggccaca
     5101 gggagctgct gaacttagtt tcggcctgaa catatcttac gtcgataacc ctacttatcc
     5161 cgaaaacatt cgccgaagcg taacttttga tttcgatgaa aaaattcaga tgaatgtcgt
     5221 cgggcagatt ggcgataaaa tgaaactcaa tgttcagtac gacaccgaat cggcttttga
     5281 cttcgaaaat tcggtaaaaa ttgaatatac cggttacgat gacgagatca ttcagaaaat
     5341 tgaagcagga aatgtgtctc ttcctctttc gggaacgctt atatcaggaa gtcagagcct
     5401 ttttggcttc aaaaccgaac tcaaattcgg aagactcaat attacaaccc tgttctcgca
     5461 gcagaaagga gaagcgtcta ctatagaggt gcaaggggga gcccagacca aggagtttga
     5521 aatatatgcc gacaaatacg aagccaacaa acactatttc ttatcgcatt atttcgagga
     5581 aaactacaac cgatttctat cgtcgttgcc tttaatcact tcaggtatta acattacaaa
     5641 agtggaagta tgggttacca accgtcaggg taacttcgaa acttcgagga atattgttgc
     5701 atttaccgac cttggtgaaa acaatccgga caacgtggaa tcgtcgtata cactgagcgg
     5761 cagtttttcg aatgttccat accctagcga ttctgccaat attctcgcca tcatcgaaag
     5821 ccttatcccc gaggtgagaa acatcaactc agcaggtaca tcccttcttg cagctggttt
     5881 cgaaggcgga gtcgattatg agaaaattga gtcagcacgc atgctttcaa cctccgaata
     5941 cactgtaaac acccaactgg gttatatttc gctgaactca accttaacct ccgaccaggt
     6001 gctggctgtt gcgtttgaat acacaatcgg cggaaaagtt ttcagggttg gtgaattttc
     6061 gaatagcgca gttaccgcac ccgatgcact tattctgaaa ttaatcaagg gaacatcatt
     6121 tacacctcaa atgaaagtgt gggatctgat gatgaaaaat atttattcta taggagccta
     6181 tcagctttcg aacgatgact tttatctgga cgtatattac aaaaacgaca aaaccggcac
     6241 cgaaataaac tttcttccgg ctggtgctat cgatagtgta cgattgctta ccgttctcaa
     6301 cctcgacgac ctgaactcac aactcgaccc gtatgcagat ggtatcttcg actttataaa
     6361 cggtgtcact gttaatgctt cgaatggtcg cattattttc ccggtaaccc aacctttcgg
     6421 aacttatctc cgacaggcta taacaggggg aaacccatcg ttgaacgagg ttgcagatca
     6481 atatgttttt caggagcttt acgacagtac ccaaagtaca gcccgtcaaa ttgctgaaaa
     6541 aaacaaattt ttcattgcag gaaaatataa atcttcttcg ggcagcgata tttcgttgaa
     6601 tgcaataaac atccctcagg gaagtgttaa agttaccgcg ggtgctcagg tacttactga
     6661 aaatgttgat tatacggtgg actataacct cggtagggtg aaaataatca accagggtat
     6721 tttagaatcg ggtacaagta ttcagatttc cattgaaagt aactcgatgt ttaatattca
     6781 aacaaaaaca ctagttggaa cccatctcaa ttacgagatt tcaaaggact tcaatatcgg
     6841 agctactatt ttaaacctta cagaacgccc gctaactcaa aaagtgagca ttggtgatga
     6901 acctatttcc aatactattt ggggtgtaaa tacatcatac cgtaccaatg ctggttggtt
     6961 gaccaaagcc atcgatttta tcccatttat cgaaaccaag gaaatgtcga caataacatt
     7021 ttccggcgaa tttgctcaat tgatacccgg acattccaaa gcaatcgaca aagaaggtaa
     7081 tgcctatatc gacgatttcg aaggtgccga aaccaacatc gacattaaaa gctactctgc
     7141 atggaccatt gctagtacac cgaacgaccc gaacatgttc ccggaacata gcggggcatc
     7201 tccgcttgat gtaggctata accgtgcaag attggcgtgg tatgtaatag atccgttgtt
     7261 ttaccggtca aattctccgg ttgatgttga actgcaatcg agccacatgg tacgcgaggt
     7321 ttacgagagc gagatatttc cctataagga aagcgttacc ggcattccga ccaacctttc
     7381 ggttatgaac cttgcctatt atccgaccga aaaaggccca tacaactact caataaatgg
     7441 catgaatgcc gatggctcac tttctgatcc gtattcacgc tgggcaggga ttatgcgtaa
     7501 actcgaaacc aacgatttcg aagaagccaa tattgaatac atcgaattct ggatgatgga
     7561 tccttttgtt gaagacagcc tgaatcccgg cggtgatatt tattttaacc tgggggatgt
     7621 ttcggaagat gtactcaaag atggaagaaa atcttttgaa cacggccttg ccactccggc
     7681 aaatcagaat ccggtagata caaccatttg gggtattgtt cccttgatac aatcgctggt
     7741 tactgccttc gataacgatg cagtatcgag acagtatcag gatgttggcc tcgatggcct
     7801 cagcgatgaa caggagcgag gtttcttctc atctgcaagc ggatggagta cctatcttga
     7861 tcagattgcc gcgttgtacg gaactggttc aatggcttat cagaaagcat taaacgaccc
     7921 ttcgaacgat aattaccatt attatcaagg ctcagatttc gactctgccg gttatggtat
     7981 tacccgcagg tattacaatt ttaacgggct cgaagggaac tcaccgtcat ctgaccagtc
     8041 gggagaagat tattcaacat cggcatcagc atcgcccaat atcgaagata ttaaccgcga
     8101 taatacgctc aacgaaaacg aaacctattt tcagtacagg gtgagcatac gtcccgaaga
     8161 tcttgttgta ggtaagaatt ttattaccga caaaatcgaa tacaacgcca agttcgccaa
     8221 taacgagcgg tcgagagtta cctggtatca gtttaaaatt ccggtgcaca gctacgaccg
     8281 cagggttggt actattcagg atttcaaatc cattcggttt atccgaatgt ttatgaagaa
     8341 ctttaatcaa aatactattg tccgtttagc aacgcttgat ctggtgaggg gggaatggcg
     8401 gaaatacaac gattcgttta tgcagcccgg cgaatataac cccgatgaac tggtggaaac
     8461 accgtttgtg gttactgcgg tgaatattga agaaaacgga aacaaaacac ctgtaaacta
     8521 tattctgcct cccgatatta accggcagat tgatccgaca aatccgcaac tccgccagct
     8581 gaatgagcag tcgatggtgt tgaaagtaat cgacttgcag gatggcgatg ggagggctgt
     8641 gtataaaaac ctcgacatgg atatgcgtaa gtacaaaaaa ctcaagatgt ttatccatgc
     8701 tgaagcgatt gtgggcgaaa ccaatttata cgacgatgac ctctgcgtgt ttatcaggct
     8761 cggcagcgat tacaaaaaca actactacga gtacgaaatc cctcttaaag ttacagcccc
     8821 cggacattac gagggaagcg aagaggacga tcccgacaga cttatcgtat ggcccgatgc
     8881 aaacaatctt agcctggaat ttgaattgct gcaggacgta aaacaaatgc gtaacgacga
     8941 aatgaggatg ctaggtaccg gtgtaaccct caccagatta tacaccataa tggacggtgg
     9001 aaataaagtg agtataatgg gcaacccaaa tctggccaat gtcagaactg ttatgatagg
     9061 ggtgcgtaac cccaaaagag caggagcaac ctccgacgac gatggtatgc cgaagtcagg
     9121 tgaaatctgg gtaaacgaac ttcgcctcac cgatttcgac gaaaagggag gctgggcagc
     9181 aaatgcacgt tttactgcaa aactcgctga ttttggttcg ctaaccattg cagggcagac
     9241 aagtaaaccc ggatttggta gcattaacga aaagcttaca gaacgccaga aggaagagat
     9301 taaccgatac gatgtgtcgt cgaaccttga attgggcaag ttcttccccg aaaaactgaa
     9361 cgtaagaata ccattgtata tgggatacag cgaaaatgta aaaaatcccg aatacaaccc
     9421 acttgatccc gacataccgc tcagtatggc acttaccgat ccgacaaaga ctccggaaga
     9481 aataaaagaa ataaaatata aatcgcagga ttataccagc ctaaaaagcc tgaattttaa
     9541 caatgttaag gtgaacaaaa cacaggggaa ggcacggatt tatgacttgg caaactggtc
     9601 ggcaagctat ggattcaacg aatcgttcca tcgcgacata aatactgtat tcaatacaaa
     9661 taaaactatc tcaggagcat tgtcgtacaa ctacaatgca acaccgaaga attatgagcc
     9721 ctttaaaaat gccaagttcc ttaataaacc tgctttcaga ctgattaagg atttcaacct
     9781 gtatctgctt ccttcgcagc tggcattcag aaccaattta aaccggcagt attccgaaaa
     9841 tcagctgagg aatgtgtata acgagtcggt tgcccttccg ctttcagtta gcaaagattt
     9901 tatctggcaa cggcagtacg acctaaaata caatatttcg aagggtttaa aaattgattt
     9961 ctctgcaacc aattcagccc ggattgatga gcctgaagga cgcatctaca ggggagatcc
    10021 tgaatacgaa tcaaagatgg acaccattta caacaacctg aagaattttg gccgaaacac
    10081 acagtatcat cacaccatcg atgttaccta tacaattcca atcaataaaa taccgctgtt
    10141 taactgggta acagccaatg cccgctatag tgccaactac gactgggtat cgggacctat
    10201 aactgccgat acaattaata tcggaaacac agttcaaaac ggtaatgcca tttcggcaaa
    10261 tgttcagttc agccttctta acctctacaa taaaattaac tatctgaaag aggtaaacca
    10321 gaaatatcga ggcaacaaga gcaaaaatgc caaaccaaag aaagaaactg tgaaattcga
    10381 agttgccgac gtaaaactca aagccaataa aaagaaaacc atcaatcata agctcaaaac
    10441 cagcgacgta agtttggttg ttgtatcggg cgatggaaaa acaattaaag gtaccaccga
    10501 aattatcgac gataataaag tgatattcac accggaagaa gatgctgata atgcaaaaat
    10561 cacagtaaat ggttccaggg aattaaaaga tggtgtagcc aaaataattt tcgacaatac
    10621 cttggtgttg ctcatgagtg taaagtcggt ttccattggc tacacgcaga acaacggaac
    10681 cctgttgccc ggatatatgc ctaacacaca gattgtgggt ttatcgaaat atacgcctga
    10741 tcccgaaatg ttcggtgtgc agccttcagt aaccgccccg acaatcccat ttattctcgg
    10801 atggcaggac agggattttg gcgagtgggc ttatgaacac aacatgctta caaaagacac
    10861 aacccttgca caggcatttg cctttatgca ttccaccaac tggaatgtga gagcctcaat
    10921 cgagccggtg cgtgatttaa gaattgacct gactttgacc cattcattct ccgaaaacgt
    10981 aagtgaatat ttcagataca ataatcagat ctctgatttc gaccgcctga atccacttac
    11041 cactggatcg tattccatgt cgactatttc gtggggaaca gccttcgaaa ttaccggacg
    11101 taagggcgat tattcatccg acaacttcca gcgtttcagc gattaccgca aggaaattgc
    11161 tgtacgcctt gcttcgcagc gaccggatta taatcccgga aacgtagatg aatttgggta
    11221 tcccattggt tttaatcagt tttcgcaaga tgtattgctc acttctttca tggctgctta
    11281 tacaggcaaa gacccgcaga aatgcaccct tgaaactttc cccaatgttt tcaatgcatt
    11341 gccaaactgg aggattactt acgacggact aggaaagata aatgccctga agaaaatttt
    11401 cagaacagtg aatatcagcc atgtgtatcg ttcaacctac aacataggct cgtacaccac
    11461 ccggttgagc gaccagtatt tcgaaaacga cggattcaac tacatccgcg aagtgctagg
    11521 ccagaaccag gaaggcgcaa tcacctatgg taacttctat tcgaaatacg aagtgaattc
    11581 ggtgaacatt tcggagcaat tttcgcccct catttcgttt gacatgacca tggtaaacag
    11641 ctttattgcc aaggtagaag taaagaagaa ccgcaacctc tccatgagct tta
//
LOCUS       DAOOQP010000090        11630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6897_length_11630_cov_4.809244, whole genome shotgun sequence.
ACCESSION   DAOOQP010000090 DAOOQP010000000
VERSION     DAOOQP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6897_length_11630_cov_4.809244"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLK75_09380"
     CDS             <1..458
                     /locus_tag="PLK75_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOY39497.1"
                     /translation="WLSMHHYNSSTDNTPSAVETLRKKGYRIIATSPRNESTTLGDFD
                     ITRGPFALFFGSEVNGLSPYVLNNADEHLSVPMVGFTESLNISVTAAICIHFLSHKLR
                     SSKVDFRLSEADKAEVFIGWLKASIKRSEKIVAEFYKTEQKNFFPPNPQ"
     gene            complement(493..3273)
                     /locus_tag="PLK75_09385"
     CDS             complement(493..3273)
                     /locus_tag="PLK75_09385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOY39498.1"
                     /translation="MKKLISSCIFLLAIVFLATAQTYPDYLPNRVIFKIRPEHRNAIS
                     DRKVHDENLKYLFEEYRVSAYYQKFIGSKAPETEFDKYKRKMIDISTIYEAELNADIF
                     AFIGKLEKLEIVEYAQPHYLPQLLYIPDDPYNTTNQFHLAKIMAYEAWDISKGDSSIV
                     IGITDTGTDIDHPDLIYQIAYNTNDPVDGADNDGDGFIDNFRGWDLAEYDNNPQADSS
                     PHGTWITGLSSAETDNGIGVAGVGFKCKYLPVKISDNENQLTMAYEGIVYAADHGCKV
                     INCSWGGSHRDALGQDVVDYAVINRDVLVIAACGNTNNQIDYYPASFDRVLSVAATTW
                     KDEKWSPLNTQTTSGSSYSYRVDISAPGAMLYSTDNGGYLTCWGGTSFASPVVAGAAG
                     VLRSYYPHLSALQIGELLKISSDNIDTVAFNEPFAGMLGAGRINMFNAITDEFTPSII
                     MEDIVVEDDRENNYENSTLVKVKGNVINYLADAENVVVQVSCSSLFVTLLTTQISIGD
                     LAAMEHYSLDEHSIQLNMAASTPVNTPVLLKLQFTADNYITVQNIEITLNPGCKNLNV
                     NDISLPVAANGRFGYYDVARKFGDGMDYREFDDLFNDCGLIAGTALNTVFCSVRQQSD
                     FLPVTSPVYVNPPESGDMQISCSMNESNDINSLGITINQNVYAWMAEEYKNLVIIDYK
                     FINTTTIPVTGLYAGIFADWDLVNPYENIASYDFQDKFIFTQHTGANTLYCAMKLLSP
                     QNENHYCLELVSGGDGLVDLTDGMSDSERFYIISNSRDEMLTDSNGEDAANVVSAGPV
                     NIAPADTATMVFAMFAAENYFDLKQTAEHAQALYNTLLYPNGITANSTEDIRVYPNPA
                     SDYLIVEIPEVLLHDAVSVCITDVSGRNVFSDCIGEKRSVIGLRWLKPGIYTVEIRAG
                     STPFVKRISVNL"
     gene            complement(3354..4454)
                     /locus_tag="PLK75_09390"
     CDS             complement(3354..4454)
                     /locus_tag="PLK75_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY39499.1"
                     /translation="MKRLILISAALFAAFGLMAQQKTANISWEKTSHDFGAFKEEAGP
                     KTAVFNFTNTGSEPLIVTNVKASCGCTTPDWTKEPVQPGQKGFVKATYDPKNRPGKFD
                     KSITVTTNTEQPTTILRISGDVTPREKTLEDFYPKAIGDLRLKTSHLAFVKVYNTKKA
                     VDSIPIVNMGTTDLKLTFSEIPAHITIKVVPEVLKGKSGNAESGQKGNIIVTYDGTKQ
                     KDFGFIMDRVVMSLNGVPQTNDRISISATVEEDFSYMSPEEKAKAPHMSFNSTEFDFG
                     KIKTGEKASYDFTFTNTGKSDLYIRKIKASCGCTATNPEKMVIKPGETSKIGVTFNSS
                     GKKGNQNKTITVIANDPDSPSIILKVKGEVLE"
     gene            complement(4494..4904)
                     /locus_tag="PLK75_09395"
     CDS             complement(4494..4904)
                     /locus_tag="PLK75_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY39500.1"
                     /translation="MKKIYLMGILLMGFIAVYAQGAVQTTTIKWEQTENNFGTFKEDA
                     GPQSFTFNFTNTGKVPLVLTDVKASCGCTTPQWSKEPVQPGKSGFVTATYNPSGRPGS
                     FSKSITVTANTDPASTVLKISGEVIPMAPPQENK"
     gene            complement(5015..5695)
                     /locus_tag="PLK75_09400"
     CDS             complement(5015..5695)
                     /locus_tag="PLK75_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOY39501.1"
                     /translation="MMLYWVIFGFFLILSVIVSSVLKSKFKKYSKIPTQGFITGREVA
                     EKMLRENGIYDVNVVSVRGQLTDHYNPLTRTINLSQDVYSGNSVAAAAVAAHETGHAL
                     QHAQSYSMLNIRSKLVPVVSFASRWVQWVLLAGIIMVNSFPQLLLAGIVLFALTTLFS
                     IITLPVEVNASSRALQWLQTARITTDENHRYAKDALQWAATTYFIAAISSLATLLYFV
                     LIYMGARD"
     gene            complement(5780..6814)
                     /gene="tsaD"
                     /locus_tag="PLK75_09405"
     CDS             complement(5780..6814)
                     /gene="tsaD"
                     /locus_tag="PLK75_09405"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOY39502.1"
                     /translation="MVNNPNILAIESSCDDTSAAVMCGSVVMSNVIASQAVHSLYGGV
                     VPELASRAHQQNIVPVVDLAIKKAGLTVSDIHAVAFTKGPGLLGSLLVGAAFAKSFAQ
                     AQGIPIIAVNHLHGHLLSNFIVEKDEAPVFPQFPYLCLLVSGGHTQLVIVRDYLQFEI
                     IGKTIDDAAGEAFDKCAKAIGLDYPGGPMVDKLAATGNELAFTFGLPRIRDYDYSFSG
                     LKTSFLYTLRDHLKDNKNFVEENMNDLCASLQKAIIDVLVAKLKKAIRDTGIKTVCIS
                     GGVAANMGLRLRLTLLSEKNHWNVFLPKKQYTTDNAAMIAVVANYCYSKGFFDELSVS
                     PLARLDVQDF"
     gene            7012..11409
                     /locus_tag="PLK75_09410"
     CDS             7012..11409
                     /locus_tag="PLK75_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOY39503.1"
                     /translation="MTRTAIQYFAEHYGLKLSIEKVYVNFPNKIVFGGVYLEDMNHDT
                     LIYANELYTKISAVKIMDSKIYVDRLVFNEAYLNLYVDTAGVNNMDFLIKKFTSNDTT
                     SSGGFDIFCNEFEFENSRFDYNDRNAPIAAKGLDVKHISLDNFNVWISDFQMYADTIE
                     LDLNEFSFYDRSGLNIEELSAHIKYNPKGIDFDQLVFRTSESILLCHKAQVEGNGDDY
                     LSDPFKKMKVNLQIDTCRISLRDISLFMPDVLCIDEYLAFQGNLKGKLSDIKLKDFSF
                     SYGENTRLNADISCSNLENFSEAFVFADISNLSTTVNEVEHLMQCFSSDPAAKLPKNI
                     HTLGLVRYQGNITGMINDLVTYGSLSTDAGDLKTDLALSYDMDKEKIAFNGKIDVYDL
                     HLGELIQDNKNFGNMSMNAKLKGEADSKGNFTANVDCTIDYIKILDYTYRNIDIDGDF
                     SNALLLGEIHVNDPNLKFNINGSYNTSGDEPVALLQMDIDANIDEVLVDTSMNASVKL
                     NMNADFRGDITEAVIGKLVVNDINVNYNGKIIALDAIKVRATEENNMQIFTVKSDYLD
                     LNLQGKYNFIELTQVFSSLVSTYLPSMVTENTEHRYFSKNDFTYELHLKNLNSIFDFF
                     IPQLRINDDWLVEGRMESSTGLFTAQSQLPRIIYDSISVEGGNFYISTNSKSIKLDMG
                     SAAVNAGKLNIFNNLAIGLHAENDSIYLIMLWDDKAEPDYSGNVSLSAILKRKENTDK
                     LLIFANIYESEININNTNWRVEETAIIIDTTDITIDNFVVFYGDQSLRIDGFLSEDKN
                     KLLRFYIENTQLNNINPLIADLGYNLDGVLTASGRVSGLYGTPVFKTNISVDQLTVNK
                     EKFGRFDLSADWLNNDQGFRLMGSNPFLRFNGNYIVEKDLMNITAEIDNFKLEVLEPY
                     LNTVDVSDTKGFIDGRINITGTTKTPELEGFIDFERAQLTYDYLKLKVNLNDTVWITP
                     NSILFNRFVVKDLENNTGTVNGGVYHDNFENFRFAFDINTNNLKMLNTTEVDNSLFYG
                     TAYGSGNVQIKGNISAFGIDVVIKTEPNTIFVLPMTESYEAGENTILTFIKSDTAVTD
                     IASLTEKESDMSYYLNMDIELTPDAEAQIVFDPKVGDMIKANCKGNLNIYYDSNEDFK
                     MIGEVEVVKGSYLFTLENVINKRFEVAEGGTIVWNGDPYDAVMNINAVYRLRAPLYDL
                     MIAIDSSSTYKKLQNVECYISMTGSLMTPDIAFDIKVPNADDKVLSQLNNMSDDEKNK
                     QLLYLLIMNRFYTPENMRPDYAQGRNASAVGNTASELLTNQLSNWLSQITKDFDIGVK
                     YRPGDEISSQELEVALSTQLLNDRILINGNLGVGQHQSQASNIVGDFEVQVKINKKGN
                     LRVKGFTRANKDLDMEYGPYTNGAGIFFTDEFNTIGDLFRKMIQWFVPDKKKKETTNS
                     DKRKTTTKWAHPNSHQSMLLNMFYPEIRS"
BASE COUNT         3483 a         2405 c         2380 g         3362 t
ORIGIN      
        1 agtggctcag catgcaccat tacaattctt ctaccgataa cactccttcg gcagtagaaa
       61 cgctgcgaaa aaaaggttac agaattattg ccacttctcc ccgtaatgaa tcgaccacac
      121 tcggcgattt cgatattacc aggggtccgt ttgcattgtt ttttggttca gaagtcaacg
      181 ggctcagccc ttatgtgctc aacaatgcag atgaacatct gagtgttccc atggttggct
      241 ttaccgagag cctgaacatt tcggttactg ctgctatatg catacatttt ctgtcgcata
      301 aactacgcag cagtaaggtt gacttcagac tcagcgaagc tgacaaagca gaagttttca
      361 ttggttggct caaagccagc atcaaacgtt ccgaaaaaat cgttgccgaa ttctataaaa
      421 ccgagcaaaa aaactttttc ccgcccaatc ctcaatgaac cagctcattg aattaccagc
      481 cccctgtgat acttataggt ttactgaaat cctttttacg aacggagttg atcctgcacg
      541 tatctccacc gtataaattc ccggtttgag ccaacgcaaa ccaataacac tccttttttc
      601 accaatacag tcagaaaaca cattcctgcc agagacatcg gtaatacata ccgatactgc
      661 gtcatgcagc aacacctctg gaatttccac tatcaggtaa tcggaagccg gatttggata
      721 taccctaata tcttcggtcg aattagcggt tataccattg ggataaagca aagtgttata
      781 caacgcctgc gcatgctctg cagtctgttt gaggtcgaaa tagttttcgg ccgcaaacat
      841 agcaaatacc atggttgcag tgtcggcggg agcaatgttc acagggcctg ctgaaaccac
      901 atttgctgcg tcttcgccgt tgctgtcggt aagcatttca tctctcgaat tcgagattat
      961 ataaaatctt tcactgtccg acatgccatc agtaagatcc accaagccat ctccaccgct
     1021 caccagttcc agacaatagt ggttttcatt ctggggcgat aacagtttca tcgcacaata
     1081 cagggtattt gcgcctgtat gctgggtaaa tataaactta tcctgaaagt cgtatgaagc
     1141 aatattctca tacgggttca ccaaatccca gtcggcaaat attcctgcat ataaccccgt
     1201 aacagggatg gttgtagtat tgataaattt ataatcgata attacaagat ttttatattc
     1261 ttcggccatc caagcgtaaa cattctgatt aattgtaatc cccaatgaat taatatcgtt
     1321 cgattcgttc atggaacagc ttatttgcat atcgccggac tcaggaggat tcacatatac
     1381 aggtgaagtt accggtagaa aatcagattg ctgccttacg gagcaaaaca cggtgtttaa
     1441 agcggttcct gcaattaaac cacaatcgtt gaataagtca tcaaattcac gataatccat
     1501 accatctccg aatttccgtg caacatcgta atagccgaac ctgccattcg ctgccaccgg
     1561 caaactgata tcattaacat tcaagttttt acatccgggg tttaaagtga tctcgatatt
     1621 ctgaaccgta atatagttat cggctgtaaa ctgcagcttg agaagaacag gggtattaac
     1681 gggagtgctt gctgccatgt tcagctgaat agagtgttca tcaagagaat aatgctccat
     1741 tgctgccaaa tcgccaatgc tgatttgtgt tgtaagtaag gtaacaaaca acgaagaaca
     1801 agaaacctgt acaacaacat tttcggcatc ggcaaggtaa ttaatcacat ttcctttaac
     1861 cttaaccagc gtggagttct catagttgtt ttcacgatcg tcttcaacca caatatcttc
     1921 cataataata gaaggtgtga attcatcggt tatagcatta aacatgttta ttcttccggc
     1981 tccgagcata ccggcaaacg gctcattaaa tgccactgtg tcgatgttat ccgagcttat
     2041 tttcagtaat tctccaatct gcagtgccga aaggtgtggg taatatgagc gtaaaactcc
     2101 ggcagccccg gcaactaccg gtgatgcaaa tgacgtccct ccccagcagg tcaggtaacc
     2161 tccattgtcg gtggaataca acatagcacc cggagcagaa atgtcaacgc gatagctgta
     2221 ggagcttccc gaggtggttt gtgtgttcag tggcgaccat ttttcatctt tccaggttgt
     2281 tgctgctacc gacagaaccc tgtcgaacga ggccgggtaa tagtctatct gattgttggt
     2341 attcccacag gcggcaatta ccaatacatc tctgttgatt acggcataat caaccacatc
     2401 ctgccccaga gcatcacgat ggctcccccc ccaggaacag tttataacct tgcaaccatg
     2461 atcggcagca tacactatac cttcgtaagc catagtgagt tggttttcgt tatccgaaat
     2521 cttcacaggg aggtatttgc atttaaaacc aacacccgca accccgatac cattatcggt
     2581 ttctgcagaa cttaatccgg taatccatgt tccgtgcggt gacgagtccg cctgagggtt
     2641 attatcgtat tctgccagat cccatcccct gaaattatcg ataaatccgt cgccatcgtt
     2701 atcggctcca tcaaccggat cgttggtatt ataggcaatc tgataaataa ggtcaggatg
     2761 atcgatgtcg gttccggtgt cggtaatacc tatcacaatg ctgctgtcgc ccttgctaat
     2821 gtcccaggct tcgtatgcca taatctttgc caaatggaac tggtttgttg tattataagg
     2881 gtcatcgggg atatacagaa gctgtggaag gtagtgtggc tgagcatact caactatttc
     2941 aagcttttca agtttaccga taaatgcaaa tatgtcagca ttcagctctg cttcgtatat
     3001 agtgctgata tcaatcattt tacgcttata tttatcgaat tctgtttctg gggccttgct
     3061 gcctataaat ttctgataat aagcggaaac ccggtattct tcaaacagat acttcaagtt
     3121 ttcgtcgtga acttttctat cagaaattgc attcctgtgc tcgggtctaa ttttaaatat
     3181 taccctgttt gggaggtaat cgggatatgt ttgtgcagtg gcaagaaaaa caattgcaag
     3241 gagaaatata caacttgaaa tcagtttttt catagttagg taatttattg aattgcaaat
     3301 taataaaaaa aggctgacaa acagccagcc tttcgttttt ttttacaatg ctcctattcc
     3361 aacacttcgc cttttacttt gagaataatg ctgggtgaat ccggatcgtt ggcgataacg
     3421 gtaatggttt tattctgatt cccttttttc ccgctggaat taaaggttac accaattttg
     3481 ctggtttcgc caggttttat caccattttt tcgggattgg tagcagtaca gccacatgaa
     3541 gctttgattt tgcgtatata caggtcgctt ttccctgtgt tagtgaatgt gaagtcgtag
     3601 ctggcttttt ctcctgtttt aattttgccg aaatcaaatt cagttgaatt aaacgacata
     3661 tggggagctt ttgctttttc ttcaggtgac atgtatgaga aatcttcttc aaccgtagcg
     3721 cttatcgaaa tcctgtcgtt ggtttgggga acaccgttaa gcgacatcac taccctgtcc
     3781 atgataaacc caaaatcttt ttgttttgta ccgtcgtaag ttacaataat gttgcctttc
     3841 tgtcctgact ctgcattgcc tgatttgcct tttaacactt caggaacaac tttaatagta
     3901 atatgagcag ggatttcgga aaaagtaagt ttcaaatcag ttgttcccat attaacaata
     3961 ggaatggaat cgaccgcctt tttcgtgtta tatactttta caaatgcaag gtgtgaggtt
     4021 ttgaggcgta aatctccgat tgcctttggg tagaaatcct caagagtttt ttctctgggt
     4081 gtaacgtcac cagaaatgcg cagaatagta gttggttgct ctgtgttggt tgttactgtt
     4141 atcgatttat cgaattttcc cggtctgttt ttggggtcat aggtggcttt aacaaaacct
     4201 ttttgtccgg gttgaactgg ttcttttgtc caatcgggag ttgtgcaacc gcacgaagct
     4261 ttcacattgg taacaataag gggttcgctg ccggtattgg tgaagttgaa aacagcagtt
     4321 ttggggccag cctcttcttt aaacgcacca aaatcgtgtg atgttttctc ccatgaaatg
     4381 ttagcggttt tttgctgagc catcaggcca aatgcagcaa aaagagctgc cgaaattaaa
     4441 attaatcttt tcatatattc aacattttaa tatttaatta actgataaat actctattta
     4501 ttttcctgtg gcggagccat aggtattact tctcccgaaa tcttcagtac ggttgaagca
     4561 ggatcggtgt ttgcagtaac ggtaatcgac ttactaaatg agcccggacg gccactgggg
     4621 ttgtatgtag cagtaacaaa tccgctttta cccggttgca cgggttcttt cgaccattgc
     4681 ggtgtggtgc aaccgcacga agctttaaca tcagtgagta ctaaaggtac ttttcccgtg
     4741 ttcgtgaaat taaaagtaaa cgactgggga ccggcatctt ctttaaatgt tccgaaatta
     4801 ttttcggttt gctcccattt aattgtggta gtttgaacag ctccctgagc ataaacggca
     4861 ataaagccca tcagtaatat gcccattaaa tagatttttt tcattttgcc ctgatttagg
     4921 ttaattttca atgtaacgtg caaacttaaa caattatcag accaaaaaaa taaaaaaagt
     4981 tgcctgcttt aacatttttt aaagaaatca gcaattaatc gcgtgcaccc atgtaaataa
     5041 ggacaaaata cagtaaagtg gcgagcgacg atattgcagc aataaaatag gtagttgcag
     5101 cccattgcaa agcatcttta gcgtaccggt gattctcatc agtagtaatg cgggctgttt
     5161 gtaaccattg caaagcacgg ctgctcgcat tcacttctac ggggagggta ataatgctga
     5221 aaagcgtggt aagggcaaac agcacaatgc cggcaagcag caactgtggg aaagaattca
     5281 ccataattat tcctgcaagc aggacccatt gtacccaccg tgatgcaaag cttaccacag
     5341 gcaccagctt tgaacgaata ttcagcatac tgtacgactg agcatgctgc agtgcatggc
     5401 cggtttcgtg agcagccacg gcagcggctg caaccgaatt cccggaatat acatcctggc
     5461 taagattaat ggttcttgta agagggttgt aatgatcggt aagctgacct ctgaccgaaa
     5521 caacatttac gtcgtaaata ccgttttcgc gtaacatttt ttcagcaact tctcttcccg
     5581 taataaaacc ctgtgttgga atctttgagt atttcttaaa ctttgattta aggacagaac
     5641 ttacaataac actcaagatt agaaaaaatc caaatataac ccagtatagc ataatttttt
     5701 aatttaattg ttccgaatgg tgatttcaaa atgtttgcca cagattaatg caagacaaaa
     5761 tgacagtttt tatcaaatct cagaaatcct gaacatcgag gcgggcaagc ggagaaacgc
     5821 taagttcatc gaaaaaccct tttgaatagc aataattggc aaccacagca atcatagctg
     5881 cattatcggt tgtgtattgc tttttcggaa ggaacacgtt ccagtgattt ttttcgctca
     5941 gcaaggtcag tcgtaatcgt aatcccatat tagcggcaac accaccgctg atgcaaactg
     6001 ttttaatgcc ggtatcacga attgcttttt tcagttttgc gaccaaaaca tcgatgattg
     6061 ctttttggag gctggcgcaa agatcattca tattctcttc aacaaagttt ttattgtctt
     6121 tcagatgatc gcgaagcgtg tacaaaaacg atgttttcag tccgctgaaa ctgtagtcgt
     6181 agtctctgat gcgcggcaga ccaaatgtaa atgcgagttc attacccgta gccgcaagtt
     6241 tatcaaccat gggaccaccc ggataatcga gtccgattgc tttggcacat ttatcgaatg
     6301 cttctcccgc agcatcgtca attgtttttc ctataatctc aaattgcaga taatcgcgca
     6361 caattacgag ttgcgtatgt ccacccgaaa ccaacaaaca gagatagggg aattgtggga
     6421 aaacaggagc ttcgtctttt tcaacaataa aattcgataa taaatgtcca tgcaaatggt
     6481 ttacagcaat aatcgggatt ccctgtgcct gtgcaaacga cttggcaaat gcagcgccta
     6541 ctaataagga tccaagcaat cccggacctt ttgtaaaggc aacggcatga atatcggaga
     6601 ctgtaagccc tgcttttttt atggccaaat cgactacagg cacaatattt tgctgatgag
     6661 ctctgctggc cagctcggga accacacctc catacaagga gtgaactgcc tgagatgcaa
     6721 ttacattcga catcactaca gaaccgcaca tcacagctgc tgatgtatcg tcgcatgatg
     6781 actcaatagc gagtatgttt ggattattaa ccataatcag ttggcaaaaa taacgaaaat
     6841 ggcgttaaaa tggttttatt ggagcaaaat ctgtcttagt tttgcattat taataatatc
     6901 gacttgggtt ggttcagatt taaaagattt ctgttggttg tagccttatg gctactgcat
     6961 tggttttttg tcatattcat cctacttcag cttccttcag tacaaagtgt tatgacccgc
     7021 acggccatac agtattttgc tgagcattac gggttgaagc tcagtatcga aaaagtgtat
     7081 gtcaatttcc cgaataaaat tgttttcgga ggtgtatatc tcgaagatat gaaccacgac
     7141 accctgattt atgccaacga gctatatacg aaaatatcag cggtaaaaat aatggattca
     7201 aaaatatatg tcgaccgcct ggtttttaac gaagcatacc tgaatttata tgtggacaca
     7261 gccggtgtga ataatatgga tttcctgatt aaaaaattca cctccaacga taccacgtct
     7321 tccggtggct tcgatatttt ctgcaatgaa tttgaatttg aaaattcacg gtttgattac
     7381 aacgaccgga atgcacccat tgctgcaaaa ggtcttgatg ttaagcatat cagccttgat
     7441 aattttaacg tatggataag cgactttcag atgtatgccg acacaatcga gcttgacctg
     7501 aacgaattta gtttttacga ccgaagcgga ctgaatatag aagagctttc ggcacatata
     7561 aagtataatc cgaagggaat tgatttcgac cagcttgttt tcaggacaag tgaatcgata
     7621 ttgttatgcc acaaagcaca ggtagaaggt aacggcgatg attatttgag cgaccctttc
     7681 aaaaaaatga aagttaatct gcaaatcgac acttgccgga tttcgttacg cgacatttca
     7741 cttttcatgc ccgatgtgct ctgtattgat gaatatctgg catttcaggg caacctgaaa
     7801 ggaaaactat ccgacatcaa gctcaaagac ttcagttttt catatggtga aaacacacga
     7861 ctgaatgccg atatcagttg cagtaatctg gaaaactttt cagaagcatt tgtttttgcc
     7921 gacatttcca atttaagtac tacggttaat gaagtggagc atctcatgca gtgctttagc
     7981 agcgacccgg cagcaaaact tcccaagaac attcacacac tgggcttagt acgttatcaa
     8041 ggtaatatta ccggtatgat aaatgatttg gtaacttacg gttctttgag caccgatgca
     8101 ggcgacctga aaaccgacct ggcattgtcg tatgatatgg acaaagaaaa gattgcattc
     8161 aacgggaaaa tcgatgtgta cgacctgcat cttggtgaat taattcagga taataaaaat
     8221 ttcggaaaca tgagcatgaa tgccaaacta aagggtgaag cagactctaa aggaaatttc
     8281 acggcgaatg ttgactgtac cattgattat attaaaatac ttgattatac ctatcgaaac
     8341 attgatatag atggcgattt cagcaacgcg ttattgctag gcgaaataca tgtaaatgac
     8401 cccaacctaa agttcaatat aaacggcagt tataatacat caggcgatga acccgtggct
     8461 ctactgcaaa tggatattga tgctaatatt gatgaagttt tggttgatac gtcgatgaat
     8521 gcatctgtta agctgaatat gaacgccgac ttcaggggcg acattaccga agcggtcatc
     8581 ggaaaactcg ttgtgaacga cattaatgta aattacaatg gaaaaataat tgcccttgat
     8641 gccattaagg taagagccac tgaagaaaat aacatgcaga tcttcacggt taaaagcgat
     8701 tatctcgatt taaatctaca ggggaaatac aacttcatcg aactaacaca agtgttttct
     8761 tcgctggttt ctacctattt accgtcgatg gtaacagaga ataccgaaca ccggtatttt
     8821 tcgaagaacg attttacgta tgagctgcat ctgaaaaacc tgaacagtat ttttgatttc
     8881 ttcataccac aactccgaat aaacgatgat tggcttgtag aaggcaggat ggaatcatcg
     8941 acaggtttgt ttacagcgca atcgcaatta ccccgtatta tctatgattc catttcggtt
     9001 gagggaggaa acttttatat ttccactaat tctaaaagca ttaagctcga tatgggatct
     9061 gcagctgtaa atgcaggcaa actaaatata ttcaataatc tggccatcgg tctgcatgcc
     9121 gaaaacgaca gcatttacct gataatgctc tgggatgata aagcagaacc cgattattcg
     9181 gggaatgttt cgctttctgc tattctgaaa cgaaaagaga acaccgacaa attgttgatt
     9241 tttgccaata tttacgaatc ggagattaat attaataata caaactggag agtcgaagaa
     9301 actgcaatta taattgatac taccgatata actatcgaca attttgtagt attctatggc
     9361 gaccaatcgc ttaggataga cggttttctt tcggaggata aaaataagct gttgcgattc
     9421 tatatcgaaa acacacaact gaataacatt aatccgctca tagccgattt gggatacaac
     9481 cttgatgggg ttttaactgc cagtggcagg gtttcggggc tttacggcac acccgtgttt
     9541 aaaaccaata tatcggtcga tcaacttacg gtgaataagg agaagtttgg aaggttcgat
     9601 ctttcggccg actggttgaa taatgatcag ggattccggc tcatggggtc aaaccctttt
     9661 ctgcgcttca acgggaatta cattgtagaa aaagatctga tgaacattac agcagaaatc
     9721 gacaatttca aacttgaggt actggaaccc tatcttaaca ctgtggatgt aagcgatact
     9781 aaaggattta ttgatggcag gatcaacatc acaggaacca caaaaactcc tgaattggaa
     9841 ggcttcatcg attttgaaag agcccagctg acctacgatt acctgaagct taaagtaaac
     9901 ctgaacgata ctgtttggat tacccccaac tcgattctgt tcaaccgttt tgtagttaaa
     9961 gacttagaaa ataatacagg aacagttaat ggaggtgtat atcacgataa tttcgagaac
    10021 ttcagatttg cattcgacat taacaccaac aacctgaaaa tgttgaatac taccgaggtt
    10081 gataacagtt tgttttatgg tacagcttat ggatcgggaa atgttcagat caaaggtaac
    10141 atttcggcat ttggcattga tgtggttata aaaacagagc ccaataccat atttgttctt
    10201 ccgatgaccg aaagctatga agccggtgaa aataccattc tcacattcat taaaagcgat
    10261 actgctgtta ccgacatagc ttctcttaca gagaaagaat ccgacatgtc gtattacctg
    10321 aacatggata ttgaactaac acccgatgct gaagcccaaa tagtatttga tccgaaagtc
    10381 ggggatatga taaaagccaa ttgcaaggga aacctaaata tctactacga ttctaacgaa
    10441 gattttaaaa tgattggaga agtggaagtg gtaaaaggga gttacctctt cactcttgaa
    10501 aatgtgatta acaaacggtt tgaggtcgcc gaaggtggaa ccatagtatg gaatggcgac
    10561 ccttacgatg ccgtgatgaa tatcaacgca gtgtaccggc tcagggcacc cctctacgat
    10621 ttaatgattg ccattgattc gagcagtacc tataaaaagc ttcaaaatgt tgagtgttac
    10681 atcagcatga ccggaagcct gatgactccg gatattgctt tcgatataaa agttcccaat
    10741 gccgacgata aagttctgtc gcaattgaat aacatgtcgg atgatgaaaa gaacaagcaa
    10801 ctgctttacc ttctcataat gaatcgtttt tatacacccg aaaacatgcg ccccgactat
    10861 gctcagggaa ggaatgccag tgccgttggg aatacagcaa gcgaactcct tacaaatcag
    10921 ttaagcaact ggctgtcgca gattaccaaa gatttcgaca tcggggtaaa atacaggcca
    10981 ggagacgaaa tttcgtcaca ggagctggag gtggctctat caacacagtt gctcaacgac
    11041 agaatcctga taaacgggaa tctgggggtc ggacaacacc agtcgcaggc aagcaatatt
    11101 gttggagatt tcgaagtaca ggtaaaaatc aacaaaaagg gcaacctgcg tgttaaaggt
    11161 ttcacacgtg caaataaaga ccttgacatg gaatatggtc catataccaa tggagccggt
    11221 atatttttca ccgatgaatt caatacgatc ggtgatttat tccgtaaaat gattcaatgg
    11281 ttcgtccccg acaaaaaaaa gaaagaaact accaattccg ataagcgcaa aaccactaca
    11341 aagtgggctc atcctaacag ccatcaatct atgctgttaa atatgtttta ccccgaaatt
    11401 cgaagttaaa catattgaat atcttctaat tttgtgcgtt tattcttaat tgaattaata
    11461 ttaattagcg gcctcatatg agcctgcaaa aattaagtat tgaacatttc agcccccggg
    11521 atttaatgtc tcacgcaaga atttactccg aaacccaagt tttaatatta acaaatacta
    11581 gtgatgcgtt aagtttttaa aatattttat aataatttat atatcagcta
//
LOCUS       DAOOQP010000091        11585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6940_length_11585_cov_5.042498, whole genome shotgun sequence.
ACCESSION   DAOOQP010000091 DAOOQP010000000
VERSION     DAOOQP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6940_length_11585_cov_5.042498"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLK75_09415"
     CDS             complement(<1..295)
                     /locus_tag="PLK75_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39504.1"
                     /translation="MKKAVLIALTVVASNLLFSQEVISNAGCSAENQSISVNYTIGEP
                     IVETFNSDDFVVTQGFNQPIITITAVEIAQCPDFSIQIFPNPTSTTLEVLVESE"
     gene            539..1663
                     /locus_tag="PLK75_09420"
     CDS             539..1663
                     /locus_tag="PLK75_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39505.1"
                     /translation="MKINDLITLFFLGISIFFTSCEDPDPDDNDNQNNTKVRYITDDI
                     DATEIWYSDTVYVVDVYNLSVEAALTIQAGTVVKFKSENGMNVLNGGSLQAIGTAEAP
                     IIFTAFNDDQFGGDVNNDAAATVPAKGYWGGIQFESVASSSLSYCKVFYAGADNPNGG
                     GPHCAIMIEHGATNLSFNHCTFAHTSGNGSSDEHCAVKILSPQLSDFVFENNTIYDNG
                     GTVVLSEAVLAGRISNTNIFHDPANAVIKNVRQGIFIYDFSFSQSIALNVTEVPYVFD
                     IVQGIDDGITITLADHVIIKFLSGAEFSYRSQGGGGFINHDGEGVLFTSIKDDTGGDS
                     NGDGNLSSPANGDWVGIKDRFNGVDYYVFQTWSNILYDQY"
     gene            complement(1773..3200)
                     /locus_tag="PLK75_09425"
     CDS             complement(1773..3200)
                     /locus_tag="PLK75_09425"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY39506.1"
                     /translation="MNKPVMWLLTIVFAITLFSCGTKSNKTHVEGTINNGEDSIILVV
                     YVDRTDTVYVGNDDAFSIDMNIDKPVWIKLIIQKNAVCLYVQPGANIVLNSDLSDFDT
                     NTKITGSDAEIENYLLTQNNFFYNHDCNNPSYKYETDISEYLGSLLAMEHQLNKNFDE
                     FQKAHGKKYKEFTDVEKLRLQYMLATLKLDYSRFVDYSSKLSDEERASYYSFMETINL
                     NNPANESIIEYNSFASLYVDWKVNTEANKEGSSILTDSDYTNMIFDIIQHDFKNSTTI
                     EYLYYVKLNDFLLYYGIDNLEPFYNEFMKIGKNQKYISEIKTLHNKWLSLGKGKPAPV
                     FNCVDAEGNSHSLSEFQGKLIVVDVWASWCGPCIREIPYLEKLKKAYLNKPVEFVAIS
                     VDDSREEWLTGLEDEKLSGNQWFVNGWNNDLCNNYIINSIPRFMVIDADGNIININAP
                     NPSGDLKQLIDSSLAELQSQTKTIQ"
     gene            3355..5040
                     /locus_tag="PLK75_09430"
     CDS             3355..5040
                     /locus_tag="PLK75_09430"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOY39507.1"
                     /translation="MKKTIILILGLMVGMQYAALACTNFLITKGASTDGSTMISYAAD
                     SHVLYGELYYFPAADYKAGDMLKVYEWDSGKYLGEIPQISHTYQVVGNVNEHQVAIGE
                     TTYGGRPELVDTTAILDYGSLIYIALQRSKTAREAIKVITDLIALYGYYSSGESFSIA
                     DKNEVWIFEMIGKGPGNKGAVWVARLVPDGYVCAHANQARITTFEYQKKNKWDDPNAN
                     TFNSPDVISFARSKKYFSGKDEEFSFSDTYAPVDFSAARFCEIRVWAFFNSVTDGMDK
                     YWDYAKGHIEHGANGYATNRMPLWVKPTKKIGVLDVMAYMRDHLENTELDMSKDAGAG
                     PFGCPYRWRPLTWQVDGQTYLNERATATQQTGFSFVAQMRSWLPDKVGCINWFSVDDA
                     ACTVYMPIYCGIAKVPYPIAHGNGSMMEFSDESAFWIFNQVSNFAYTRWNSIYPEIKG
                     LQYDLEKGFTKETAVIDSKIANMKSISDAEITDIVTQYSVTAVEMTFKTWKKLYSDLF
                     VKYMDGNIKEKNPVPDNYLYVTPSVKQPGYSYSFLKNIVDETKDKLKVVGPAH"
     gene            complement(5095..6024)
                     /locus_tag="PLK75_09435"
     CDS             complement(5095..6024)
                     /locus_tag="PLK75_09435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOY39508.1"
                     /translation="MKFIISVLKQHFRDDFSRKGVVVSLLIAAILILAESVFKVNERI
                     TNEHSYTFIIYLWDFLRNAFVYYGSTAVLSATGIFHVENKKRFVAVSLVGIILFSIYS
                     VFYGYIYAVSNAPYPCRLWLHSICENISGIFTLFLPLLLFWYFFQRREVNGFYGLHFR
                     NLQFVRYADVILLVVICMFFAMFLPGVSEYYPVLNDTYYRNCANYIGLSQWVTATGFE
                     ISYLFDFVWIELFFRGFLVFGMVKFLGKNVILPMIVLYVVIHFGKPLPEIISSFFGGY
                     LLGILALHTGNIRTGILLHITLALSAEVFGMLL"
     gene            complement(6057..7073)
                     /locus_tag="PLK75_09440"
     CDS             complement(6057..7073)
                     /locus_tag="PLK75_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39509.1"
                     /translation="MIKKLFLGCLITLIFFQSVNAQWAECVPEPSVTDPEGVGVRYPY
                     LLPVGFNNEYYHTVLTIISPVSAETWGNFSVTITRIQLTEITNIPEGLTWETNSGNAD
                     DYLYGGEKYCLVLEGTPNSTPGIRTVTVYANAWIKLLWGLFETAAPGNPQEGGTVKYT
                     LCNELNLELGNDRTITTNDEFTLSADQNTSFHHYLWQDSTTNPTYTIRGSVLGVGTHK
                     IKVTVSDTVGTTGVHDGEVTRCFKSDSINITVINSNAIDLSATGKISIFPNPGKDEII
                     ITQIDELRNTTVSIFDIRGVKVLEVVLDKPEQRFDISSLSNGIYFVKFSSDRLNDVVK
                     FLKE"
     gene            complement(7198..8904)
                     /locus_tag="PLK75_09445"
     CDS             complement(7198..8904)
                     /locus_tag="PLK75_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOY39510.1"
                     /translation="MANFFFDNKNLEFHLHHPLMKKIVPLKEANYRDYGIYDDAPADY
                     EDCMDSYEKVLEIIGEICGEIIAPNADDVDKEGPVVENGRVIYAKGTAQNHEALTKAG
                     VVGMALPRKFKGLNFSMVPYVMAAELVSRADAGFANIWGLQDCGETLLEFASDDIKNK
                     YLPMFNEGATASMDLTEPDAGSDLQAVRLKAHFDEKSQQWLLNGVKRFITNGDAEIAL
                     VLARSEEGTVDGRGLSLFVYDKNDNAVTVRRIEHKLGIVGSPTCELVFHNAPAQLVGE
                     RKLGLIKYVMSLMNGARLGVAAQSVGIAEAAYREAVKYASERKQFGKLINSFPAVYEM
                     LKMMQVNIFAIRSLLYETTRFVDMYKCYQFLAEERKLEKEEISDQKKYNRLADVFTPL
                     VKLVSSEYANQIAYDSLQIHGGTGYMKDFPIERIYRDARITSIYEGTTQLQVVAAIRG
                     VNTGTFLSQINEYASIEVKPEHEYLKFHLEEMTQTFREAQEKVNSAGNLEFLDFHARR
                     LVEMCGNIIMGYLLLHDAERDKEYSLAAEIFILDAQSKNRMKANIISSFDMPLANKFK
                     QL"
     gene            complement(8909..9928)
                     /locus_tag="PLK75_09450"
     CDS             complement(8909..9928)
                     /locus_tag="PLK75_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOY39511.1"
                     /translation="MNNICVICETNGGKVAEVSLELLCKAHELAGTIGCKVEAMLIGH
                     DLGPASTELTKYGADKVLIADDVRLKVYQSLPYTAIVVGVLKETQPVIALLGATNAGR
                     DLGPRVSSALHSGLTADCTDLQIGEHYEKKNDTTYRNLLYQIRPAFGGNIIATIINPD
                     HRPQMATVREGVMKKQIFSGRKPSEIVGLDVNKYLHDSDFVVKIIERSIEESKTDIKS
                     APVLISGGYGVGSKENFALLFELAELLGGEVSGSRAAVDAGFISHDRQVGQTGVTVRP
                     KLYIACGISGQVQHTAGMNQSAMIISINNDPNAPINEIADYVITGDLNSVIPKIIEAY
                     KKNKK"
     gene            complement(9980..10843)
                     /locus_tag="PLK75_09455"
     CDS             complement(9980..10843)
                     /locus_tag="PLK75_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOY39512.1"
                     /translation="MKALKIVVLAKQVPDTRNVGKDAMKADGTVNRAVLPAIFNPEDL
                     NALEQALQIKDALPGTHICLLTMGPPRAAEIIREGLFRGIDEGILLTDRKFAGSDTLA
                     TSYALALAVKKLNPDIIIAGRQAIDGDTAQVGPQVAEKLNFPQITYAEQIYILGSTIR
                     VKRRLEHGSELVECPLPALVTVGSSAPVCRTRNAKLTLKYKHAATNTELQEQSSDYFM
                     HNKPYLKITEWGVDEIGADAEQLGLSGSPTKVKKIENVVFSAKDTIQLNDNYTEVESM
                     VKDLITSHIIG"
     gene            10894..11568
                     /locus_tag="PLK75_09460"
     CDS             10894..11568
                     /locus_tag="PLK75_09460"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39513.1"
                     /translation="MHDARLCCCDSLPLDFVRDMLDNADICAISTPAGQGGIAVIRVS
                     GDNALVIAQKIIESNKPRISGFSPRKVYLCNIVEGSDLIDEVLFVWFKAPYSYTGQDM
                     VEISCHGSLYIQHKIIDMLIVAGARIAMPGEFTQRAFLNGKIDLSQSEAIADLIASGA
                     KASHRLAINQMKGKLSNEILLLREKLLEFISLIELELDFSEEDVEFADRKQLNALIAV
                     LIARIS"
BASE COUNT         3355 a         2334 c         2345 g         3551 t
ORIGIN      
        1 tttcactttc aacaagtact tcaagggtag tggatgtggg attggggaat atttgaatgg
       61 aaaaatcggg gcattgagct atttcgacag ctgtaatagt aataattggc tgattaaacc
      121 cctgtgtaac tacaaaatca tcagaattga atgtttcaac aatgggttcg ccaatagtgt
      181 agttaacact gattgactgg ttttcggcag aacatccggc attggaaatt acttcctgac
      241 tgaacagcag gtttgatgca acaacagtta atgcaattaa aacagccttt ttcatattga
      301 ttataatttt attaataatc aaatataatg tataaaaagg tatactacat tgcttcggtt
      361 aaattgttaa catattgtac tttgttttag tgctttttaa cttaatagtg caatttattt
      421 atcgaatatt cttggtataa ttatcaaata tttagagaat aagctcaact ccgttttttt
      481 tataaatgta atatcctggc attaaaaaat atcaaacata aactacaact caataactat
      541 gaaaataaat gatcttataa ctctgttctt ccttggtatt tcaatttttt ttacatcttg
      601 cgaagaccct gaccctgatg ataacgacaa tcagaacaac acaaaagtac gttacataac
      661 cgatgatatt gatgcaactg aaatatggta ttccgacacc gtatatgttg ttgatgttta
      721 caatctctct gtcgaggccg cactaacaat tcaagcgggg acagttgtga agttcaaatc
      781 ggaaaacgga atgaatgttt tgaatggggg tagcctacag gctattggaa cggctgaagc
      841 tcctattatt tttacggctt ttaacgatga tcaatttggc ggtgatgtga ataacgatgc
      901 tgctgccact gtcccggcca aagggtattg gggtgggatt cagtttgagt cagttgcttc
      961 aagttccctt tcgtattgca aagtttttta tgctggtgcc gataacccca acgggggagg
     1021 accacattgt gccatcatga ttgagcatgg agcaactaac ctgagtttca atcattgcac
     1081 ttttgcccat acttcaggga atggcagcag cgacgaacac tgtgcagtca aaattctaag
     1141 tccgcaactt tccgattttg tttttgagaa taatactatt tacgataatg gtggtacggt
     1201 ggtactaagc gaagcagtac tagcaggcag aatcagtaat actaatatat ttcatgatcc
     1261 agccaatgct gtaatcaaaa atgtacgtca gggaattttt atttatgact tctcatttag
     1321 ccagagcatt gctctgaatg taactgaagt accatatgtt ttcgatattg ttcagggcat
     1381 tgatgatggg attacaatta ctctcgctga ccatgtgatt attaaatttc tttccggggc
     1441 tgaattttct tatcgaagtc agggtggagg cggctttata aatcatgatg gagaaggtgt
     1501 gctcttcacc tcaataaaag atgacactgg tggcgacagc aatggggatg gaaacctgtc
     1561 aagccctgca aatggtgatt gggttggtat taaagaccgc tttaacgggg ttgattacta
     1621 tgttttccaa acatggagca acatactgta cgaccagtat taatattacg ttgaggtgcc
     1681 atcaattaat aagcaccccg tacatgcaat gttttataat taagaaatga agttactatg
     1741 ctggcattaa ctgaatggga ttttccggta tatcactgaa tcgtttttgt ttgactttgt
     1801 aattcagcca aacttgaatc gataagctgt tttaaatcgc ccgaagggtt gggtgcattg
     1861 atgtttataa tattaccgtc agcatcaata accatgaatc gtgggatact gttgatgata
     1921 taattattgc acaggtcatt gttccagccg ttaacaaacc actgattgcc tgaaagcttt
     1981 tcatcctcaa gtccggtaag ccattcctcc ctgctatcgt caacagagat ggctacaaat
     2041 tcaaccggtt tgttcagata tgcttttttt aacttttcca aataaggtat ttcgcgtatg
     2101 catggtccac accacgaagc ccagacatca accacaataa gctttccctg gaattcgctg
     2161 agagaatgtg aattaccctc ggcgtcaaca cagttgaaaa caggggcagg cttgcctttg
     2221 ccaaggctta accatttgtt gtgcaaggtt ttaatttcag agatgtattt ttgattttta
     2281 ccaattttca taaactcatt atagaatggc tcgagattat cgattccgta ataaagcagg
     2341 aaatcgttaa gttttacata ataaaggtat tcaatagtgg tactgttttt aaaatcgtgc
     2401 tgaattatat caaaaatcat atttgtataa tcgctgtcgg taagaatgct gctgccttct
     2461 ttgttggctt cggtatttac tttccaatcg acgtatagtg aggcaaagct attgtattca
     2521 atgatgcttt cgtttgcggg attattgaga tttatggttt ccataaagga ataatagctc
     2581 gcacgctctt cgtcgctcag tttactgctg tagtctacaa aacgactata gtccagtttt
     2641 aatgtagcca gcatatattg taagcgcaat ttctcaacat cagtaaattc tttgtatttt
     2701 ttaccgtgtg ctttctggaa ttcatcgaag tttttgttca actgatgttc catggccaat
     2761 aatgaaccca gatattcgct gatatcggtc tcatatttgt aactgggatt attgcagtcg
     2821 tgattataga agaaattgtt ctgtgttaac aggtaatttt caatttctgc atcagagcct
     2881 gttattttgg tattggtatc aaaatccgac aaatctgaat taagaactat attagctcca
     2941 ggctgtacat acaaacaaac tgcatttttc tgaataatca gctttatcca aaccggttta
     3001 tcaatgttca tatcaattga gaaagcatcg tcatttccaa cataaaccgt atcggttcga
     3061 tcgacataaa ctacaagaat gattgagtct tccccattgt ttatcgtgcc ttccacgtgc
     3121 gttttattgc tctttgttcc acacgaaaac agcgttatgg caaacactat agtcagaagc
     3181 cacattaccg gtttattcat aataaatatt ttttttgcaa tttaggtata aattttggaa
     3241 gcaggtaaac cggacaaaat aaaatataaa cacgatttta tataaaatat tttttgtttc
     3301 ttttatatta acctaataat tttatttttg tgtcaataaa aacaaaaacg gcaaatgaaa
     3361 aaaacaatta ttctaatttt agggcttatg gtgggaatgc aatatgcagc ccttgcatgc
     3421 accaactttc ttataactaa aggagcctca accgatggtt caacaatgat cagctatgct
     3481 gccgactcac acgttcttta tggtgagctg tattattttc ctgcagctga ctacaaagca
     3541 ggagatatgt tgaaagttta tgaatgggat agtggtaaat atctgggtga aataccacag
     3601 atatctcata catatcaggt ggtcggaaat gtgaacgagc atcaggttgc tattggtgaa
     3661 actacctatg gcggaagacc ggaattggtt gatacaacag ctattcttga ttatggatct
     3721 ctcatataca tagcattgca acgctcaaaa acagccagag aggcaataaa agttattacc
     3781 gacctgattg ctttgtatgg ttattacagc agtggagaaa gcttttcgat tgccgacaaa
     3841 aatgaggtat ggattttcga aatgattggg aaaggacccg gtaacaaagg tgctgtgtgg
     3901 gttgcccgcc ttgttcctga tgggtatgtt tgtgctcatg ccaaccaggc acgtatcact
     3961 acttttgaat atcagaagaa aaataaatgg gatgacccga atgccaatac tttcaattcg
     4021 cctgatgtta taagctttgc cagaagcaag aaatattttt cgggaaaaga cgaagaattc
     4081 agtttcagcg atacatatgc gccggtcgat ttcagtgcag cacgtttttg cgaaatcagg
     4141 gtttgggcgt tctttaattc cgttaccgat ggtatggaca aatattggga ttatgcaaaa
     4201 gggcacattg agcatggtgc taatggatat gcaaccaaca gaatgccttt gtgggtgaaa
     4261 cctacaaaaa aaatcggtgt tcttgatgta atggcttata tgcgcgatca cctcgaaaat
     4321 accgaactcg atatgagtaa ggatgccggt gccggtcctt ttggttgtcc gtaccgatgg
     4381 agaccgctta catggcaggt tgacggccaa acctatttga atgaaagagc aactgcaaca
     4441 caacaaacag gattttcgtt tgttgctcaa atgcgctcat ggctacccga caaagtgggt
     4501 tgcatcaatt ggttcagcgt cgacgatgca gcttgcactg tatatatgcc aatttattgc
     4561 ggtattgcta aagtgcccta cccaatagca cacggtaatg gttcaatgat ggagttttcg
     4621 gatgaatcgg cattttggat ttttaatcag gtttcaaatt ttgcttatac acgatggaat
     4681 tctatttacc cggaaatcaa aggcttgcag tatgaccttg aaaaaggatt tacaaaagaa
     4741 acagctgtta ttgactctaa aatcgcaaat atgaagagta tcagcgatgc tgaaataaca
     4801 gatattgtta cgcaatactc tgttactgca gtcgaaatga cttttaaaac atggaaaaaa
     4861 ctttattccg acttgtttgt gaaatatatg gatggcaata taaaagaaaa gaaccccgtt
     4921 cctgataatt atttatatgt aacaccatcg gttaaacaac ccggatatag ttatagtttt
     4981 ctgaagaata ttgttgacga aacaaaagat aaactaaaag ttgttggacc agcccattaa
     5041 ttgacctcat atataattat gcagctgccc gtataaaatc tggcagctgc ttttttatag
     5101 aagcattcca aaaacttctg ccgaaagcgc caatgtaata tgaagtaata tacctgttct
     5161 aatgttaccg gtgtgtaacg ctaatatacc gagcaggtag cctccgaaaa acgacgatat
     5221 aatttcgggt agtggtttac cgaaatgtat tacaacatac aatacaatca taggcagaat
     5281 aacattctta cctaggaatt taaccatccc aaaaaccaga aatcccctga aaaacagctc
     5341 aatccatacg aaatcgaaaa gatacgatat ctcaaaaccc gtagcggtta cccattgtga
     5401 taggccaata tagtttgcac agtttcggta ataggtatcg ttcagtaccg gataatactc
     5461 tgaaactccg gggagaaaca tggcaaagaa catgcaaata acaaccaaga gaatcacatc
     5521 ggcgtatcgt acgaattgca gattacgaaa gtgaagtccg taaaaaccat tgacttcccg
     5581 tcgctgaaaa aaataccaaa acaacaataa aggcagaaac aatgtgaata tgccggagat
     5641 gttttcgcaa atagaatgca accataaccg gcaagggtag ggggcgttac ttaccgcgta
     5701 aatatacccg taaaacacgc tgtagatgct gaataatata atcccgacaa ggcttactgc
     5761 aacaaatcgt tttttatttt ctacatggaa tatgccagta gcagataata cagcagtact
     5821 tccgtaatat acaaacgcgt tgcgtaagaa atcccataaa tagatgataa aagtatagga
     5881 atgttcattg gttatccttt cattaacttt aaatacactt tctgccagaa ttagaattgc
     5941 tgctataagt aacgaaacaa ctactccttt tcgtgagaaa tcgtcacgaa agtgctgttt
     6001 tagtaccgat attatgaatt tcatactgcc ggtgttatcc agcaattatt aataaattat
     6061 tctttcagga attttacaac atcgttgagt ctgtcgctgc tgaatttaac aaaataaata
     6121 ccattactta agcttgatat atcgaatctc tgctccggtt tatcaagcac tacttcgagc
     6181 acctttactc ctcgaatgtc gaaaatggag accgttgtgt tccgcaattc atcgatctgg
     6241 gtaattataa tttcgtcttt acccgggttg ggaaaaatgg agattttccc tgttgcagaa
     6301 aggtctatag cattgctatt gataactgta atgttgatac tatccgattt aaagcaacgg
     6361 gttacttcac cgtcgtgtac gcctgttgtt ccaactgtat cggaaactgt aaccttgatt
     6421 ttatgcgttc ccactcccaa caccgatcct cgaatagtat aagtgggatt agtggttgaa
     6481 tcctgccaga ggtaatgatg aaaactggta ttttggtcag cacttagtgt aaattcatca
     6541 ttagtagtta tggttctgtc gttgccaagt tccaaattga gctcattaca taaggtatac
     6601 ttcacggttc cgccttcctg tggatttccg ggagctgcag tttcaaacaa gccccacagc
     6661 agttttatcc atgcattggc atacaccgtt actgttctga tgcctggagt gctgttaggg
     6721 gtgccttcca gtacaagaca atacttttca cctccgtaca aataatcatc cgcattgcct
     6781 gaattggttt cccaagtaag cccttcgggt atatttgtaa tttcggtaag ctgaatgcgt
     6841 gtgatagtaa cactaaaatt tccccaggtt tcggcactaa ccggtgatat aattgtaagc
     6901 actgtatggt aatattcgtt attaaaacca acaggcagca ggtatggata cctcactcca
     6961 acgccttcgg gatcggtaac cgaaggttca ggaacgcact ctgcccattg ggcattgacc
     7021 gattgaaaaa aaataagggt gatcaaacat ccaagaaaga gtttcttaat catttcaata
     7081 gtgatgtgtt atgaacaatt ttgtaacaaa actataaagt aaatttgaaa ataaaagacc
     7141 atgttgcaat aaaaattata acaacatact gttaatgatt tgatattgat ttttctgcta
     7201 taattgcttg aatttgtttg ccaatggcat gtcgaaactg cttattatat ttgctttcat
     7261 cctgtttttt gattgagcat ccaaaataaa tatttcggct gcaagagaat attctttatc
     7321 gcgttctgca tcatgtagca acagatagcc catgattata tttccacaca tttcaaccag
     7381 ccgacgggca tgaaaatcga gaaactcaag attacctgct gaattaactt tttcctgagc
     7441 ttcgcgaaaa gtttgggtca tttcttcgag gtgaaatttt agatattcat gctcaggttt
     7501 cacttctatt gaagcatatt cattaatctg cgacagaaat gtaccagtat ttacacccct
     7561 gatagctgcc acaacctgca gttgagtagt gccttcgtaa atggatgtga tgcgcgcgtc
     7621 gcggtaaatg cgctcgattg ggaaatcctt catatagcca gtcccgccat gtatctgcaa
     7681 gctatcataa gctatctgat tggcatattc gcttgaaacg agtttaacca gaggagtaaa
     7741 tacatcagca agccggttgt attttttttg gtctgaaatt tcttcctttt cgagcttgcg
     7801 ctcttctgca aggaactgat agcatttgta catatctaca aaacgtgttg tttcgtacaa
     7861 taggcttcta attgcaaaga tatttacctg catcatttta agcatttcgt acactgctgg
     7921 aaaactgttg ataagctttc cgaactgttt acgttcagat gcatatttca cagcttcacg
     7981 gtaagcggct tcggcaatgc ctaccgactg tgcggcaact ccgagccttg ccccgttcat
     8041 cagcgacatt acatatttta tcagtccgag tttcctttcg ccaaccaatt gtgcgggtgc
     8101 attatggaaa accagctcgc aagtgggaga gcccacaatt ccaagtttgt gttcaatgcg
     8161 ccttaccgta acggcattat cgtttttatc gtaaacaaac agcgaaagtc ccctgccatc
     8221 gacagtacct tcttcggagc gcgcaagcac aagggctatt tcagcatccc cattggttat
     8281 aaatcgcttt acaccgttca gcaaccactg ctgactcttt tcgtcgaagt gagctttcag
     8341 tcgcactgct tgaagatcag agccggcgtc aggttccgtg agatccatcg aggctgtggc
     8401 gccctcatta aacatcggga gatatttgtt tttaatgtcg tcggaggcaa attctagcaa
     8461 tgtttcgccg cagtcctgca atccccagat attggcaaaa cccgcatcag ctctcgaaac
     8521 cagttcagca gccatcacat agggtaccat cgaaaaattc aaccccttaa atttacgcgg
     8581 taatgccata ccaacaacac cggctttagt tagcgcttca tgattttggg ctgttccttt
     8641 agcatatatt accctgccgt tttcaaccac cggaccttcc ttgtcgacgt catcggcatt
     8701 cggggcaatg atttcgccgc atatttctcc tattatttcc agcacttttt cataactgtc
     8761 catgcaatct tcgtaatcgg caggggcatc gtcgtatata ccatagtcac ggtagttcgc
     8821 ttctttaagc ggaactattt tcttcatcaa tgggtgatga aggtgaaact caaggttttt
     8881 attgtcgaaa aaaaagttag ccatagcatt attttttgtt ctttttatat gcttcaatta
     8941 ttttaggaat cactgaattc agatcaccgg taattacata atcggcaatt tcgttgatag
     9001 gcgcattcgg gtcgttattg attgaaatga tcattgccga ctgattcata ccggcagtat
     9061 gctgcacttg cccactgata ccgcaggcaa tatatagttt tgggcggacg gttacgccgg
     9121 tttgcccaac ttgcctgtcg tgcgaaataa aacctgcatc aacagctgct cttgaccctg
     9181 atacttctcc ccctagtaat tcagcaagtt caaaaagcaa agcaaagttc tctttcgacc
     9241 cgacaccata ccctcccgaa atcagtacag gcgccgattt aatgtcggtt tttgattctt
     9301 cgatgcttct ttcaatgatt tttacgacaa agtcgctgtc gtgcaaatat ttattaacgt
     9361 caaggccaac tatttcgcta ggttttcttc cgctgaaaat ttgttttttc ataactcctt
     9421 cgcgaacagt agccatctgt ggccggtggt cagggttgat gatggtggct ataatgtttc
     9481 caccgaacgc gggacgtatc tggtacaaca gatttcggta ggtcgtatcg ttttttttct
     9541 cgtaatgttc accaatctga aggtcggtgc aatcggctgt tagtccgctg tgcaatgcgg
     9601 aagaaaccct tggacccaaa tctctccctg catttgtagc ccccagcaat gcaattacgg
     9661 gttgtgtttc tttaagaact ccaacaacaa ttgctgtata aggcaacgat tggtaaacct
     9721 taagccgtac atcgtcggca atgagtacct tgtcggcacc atacttggtt aattcggtgg
     9781 atgcgggtcc caaatcgtga ccaatcaaca ttgcttcaac cttgcatccg atagttccgg
     9841 caagttcgtg tgctttacaa agtagttcaa ggctgacctc agcaactttg cctccatttg
     9901 tttcacatat cacacaaata ttattcattt taagcttgtc taattatttt aacttcgttt
     9961 tagtttccaa ctttcagtat tatccgataa tatggcttgt aatcaaatct tttaccatcg
    10021 attccacttc ggtgtaattg tcgtttagtt gaatggtgtc ctttgccgaa aaaacgacat
    10081 tttcaatctt cttgacttta gttggagaac cgcttaatcc cagttgttca gcgtcggcgc
    10141 caatttcatc aacaccccat tctgtaattt tcaaataggg cttattatgc atgaaataat
    10201 cgctgctttg ctcctgaagc tctgtattgg tagctgcatg tttatatttc agtgtaagct
    10261 ttgcattccg cgtccggcaa acaggtgcag agcttccaac tgttacgagc gcgggcaggg
    10321 ggcattctac caattcgctg ccgtgttcaa gtcggcgttt taccctgata gtactaccta
    10381 ggatgtaaat ttgttcggca taggtaattt gaggaaaatt taatttttcg gcaacctgtg
    10441 ggcctacctg ggctgtatcg ccatcaatag cctgacgccc agcaataatg atatcgggat
    10501 tgagtttttt cacggcaagg gcaagggcat acgaagttgc taaagtatcg ctgcctgcaa
    10561 atttacggtc agtaagcaaa ataccttcgt cgatgccacg gaacagccct tctctgatta
    10621 tttcagcagc acgtggcgga cccatggtta gtaaacaaat gtgagtaccg ggcagggcat
    10681 cttttatctg taatgcctgc tcaagtgcat tcaggtcttc cggattgaaa atagcgggta
    10741 acacagccct gtttactgtg ccgtcggctt tcatggcatc tttgccaaca ttccttgtgt
    10801 caggtacttg ctttgccaga actactattt ttaatgcttt cataggttaa aaatatgctg
    10861 caaagatata aaactgtttt aaacagcaaa tttatgcacg acgcgaggtt atgttgctgc
    10921 gattcattac ctttggactt tgtaagagat atgctcgaca acgctgacat atgtgccatt
    10981 tcaactccag cagggcaggg tggtattgct gtgatcaggg tgagcgggga caatgctttg
    11041 gtgattgctc aaaaaatcat tgaaagtaac aaacctcgca tttccggctt ttccccccga
    11101 aaagtatatt tgtgcaacat cgttgaaggc agcgatctta tcgatgaagt gctgtttgtt
    11161 tggttcaaag ctccatactc ttacaccggc caggatatgg tggagataag ctgccacgga
    11221 agcctctaca tacagcataa aattatcgac atgcttattg tcgcaggagc gagaattgcc
    11281 atgccgggag agttcacaca acgggctttc ctgaatggaa aaatcgacct tagtcagagt
    11341 gaggctattg ccgacctgat agcttcggga gcaaaagcaa gtcacaggct ggcaataaat
    11401 cagatgaaag ggaagcttag taacgaaatc ctcctgttgc gggaaaaact tctcgaattc
    11461 ataagcctga ttgaacttga gcttgatttt tcggaagaag acgttgaatt tgccgaccgg
    11521 aaacaactga atgctcttat agctgtactc attgcccgta tttcgtaatt actggcaact
    11581 tttcg
//
LOCUS       DAOOQP010000092        11513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_6994_length_11513_cov_6.076715, whole genome shotgun sequence.
ACCESSION   DAOOQP010000092 DAOOQP010000000
VERSION     DAOOQP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_11513_cov_6.076715"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLK75_09465"
     CDS             complement(<1..629)
                     /locus_tag="PLK75_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39514.1"
                     /translation="MKNTVLILITAMCIVMHSTAVAQLQSVLSDAPIACYFEQDAYNS
                     FPDGDDAPSPNIESHISGQINRDKGDMKNYSPDFEPELTSFLASSGCSGFSVTATGGT
                     TICSGSSAQLDVSVPSALNYSYTSTYNYSGTDCDQSYAITYFNCGQTNSVITNIEYTI
                     SGLPGCSSGWYSADVYFDGGLADEYLCDGTYSYSGLNGLDATGVHNFGVV"
     gene            complement(845..1654)
                     /locus_tag="PLK75_09470"
     CDS             complement(845..1654)
                     /locus_tag="PLK75_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOY39515.1"
                     /translation="MFVTDYDGTLASTSGFVSLANIDALERLGEKGIVRVIATGRSLF
                     SLFQVIDFTFPVDYVIFSSGLGIINLKTKKIRSKSTLEKSDTKLIMKWLSQNGYNFML
                     HKAPPDNHRFYAYTAEYEHDDFQRRLAHYMRLDVKLLVDPPELASQFVVICNDDEGHF
                     QHIQSAFNNFKVIRTTSPFDGISLWVEIFPYDVSKAHGITEICNELSIESKNVHVVGN
                     DFNDSDMLEWSVNSFVVENGSEALKQIYTVIPSNTDNGIAFLINNLLANTV"
     gene            complement(1690..2358)
                     /locus_tag="PLK75_09475"
     CDS             complement(1690..2358)
                     /locus_tag="PLK75_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39516.1"
                     /translation="MGTYQKIFHYQRLILIVFYVLFCCMSIYPIILILQKMGVSNMAV
                     LILIPFLFVVICTIAGAYLYVISHIPLKLVDAFDDIRNKIASEEIHTVEQFATHVNRF
                     TVSFFSFMFFDVHHSVFIIGDKRNIYVSDKLAGEDLQNVDDLWQYAAQNENIRYEGTQ
                     KIQGINEHRVVVPIWFGKEFLGLMLLYIEQKPGKFFTGMIRDYEELFIDDQLLHIVNL
                     NKHK"
     gene            2455..2811
                     /locus_tag="PLK75_09480"
     CDS             2455..2811
                     /locus_tag="PLK75_09480"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOY39517.1"
                     /translation="MDITGKITHILPVQTGEGKNGTWKKQLFVIETTDEFPKSVCFTA
                     WGDRVELSKMSVGEQIKVYFDLESREYNNKWFTDAKAWKAESIGAANGTVTPTAYDQP
                     EAIKNLEPEEGDDLPF"
     gene            complement(2834..4825)
                     /locus_tag="PLK75_09485"
     CDS             complement(2834..4825)
                     /locus_tag="PLK75_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1736 domain-containing protein"
                     /protein_id="HOY39518.1"
                     /translation="MKKNRLPYYILFGLSVMLYLNTIPNRYALDDKMIIFKNNYTQKG
                     FAGIGDILTKDAMAGMFGDNNQAVAGGRYRPLSMISFAVEQEIFGGNPHVSHFFNLLF
                     YALSVIVLYRLLLRLCKDNNKTKWYFSIAFIASLLFAVHPLHTDAVANIKGRDEIFCF
                     LFVLLSWDSILKYTDTKSKGHIAISFIWFLLAVFSKETAITFLFIIPVSMYFFRKPNR
                     KQYLTSFFSIAGASIIYLITRFAVLGNTLGNEVGELMNNPFLEATTGEKYATIFMTLG
                     IYLKLLVFPHPLTWDYYPYHIPLIEWSDWRAILPLLLYVALGVVAIVGTLRKSPYAFA
                     VWVYLATLSITSNLFFTVGAFMSERFLYMSLLGFCLAIAYFVNTRMVKTKLLQRVAIG
                     TLLLICLAFSGKTIIRNFDWKDNLTLFGHDVAVSVNSAKGNSSYASELYSLAETQTDT
                     AERNKILKSAMPYFRKAIEVYPSYTEPLIRLGNCYYIMYGDYKTMFDYYLKALRADPN
                     NTDVWGNAIGVLQNNVTDPKFEIAFWTEVSKVAPNRFESFWYMGEQYRLAENRPDSAV
                     IYLEKANSMNPGNFDILKSLGIVYGSLNRFNLARETLLKAVSINPNDAECHRYIGVSY
                     GIEENDSLALMHFEKAYKLNPNDVQNQNNLLIARKRLGL"
     gene            4986..5936
                     /locus_tag="PLK75_09490"
     CDS             4986..5936
                     /locus_tag="PLK75_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOY39519.1"
                     /translation="MDYEVILQNIWWIILILIILFSGLKTVSQGTIAVITLFGKYRRI
                     MRPGLNFKIPVIEKIARRISIQNRSVELEFQAVTQDQANVYFKSMLLYAVQNENEETI
                     VKVAFKFYSDKDLMQALIRTIEGNIRSYVATKKQAEILALRKEIVDSVKLEVDHVLED
                     WGYHLLDLQINDITFDAAIMESMSKVVASNNLKAAAENEGQALLITKTKAAEAEGNAI
                     KISAQAEMQAAQLRGQGVALFRQEVAKGMSEAAEQMKQANLDTNVILFSMWTEAIKNF
                     AEYGKGNIIFLDGSSEGMQHTMKQIMGLMKMDEGNQGKNH"
     gene            complement(5962..8901)
                     /locus_tag="PLK75_09495"
     CDS             complement(5962..8901)
                     /locus_tag="PLK75_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOY39520.1"
                     /translation="MLKKIILHLLWLSIVLMPLSLFSQNLSRDEVRNLAILFYKEINK
                     QCNDSLISDELFIRTGNSENIAIFCFQSGGFVALSMYKGVAPIIAYSGEGFHDLNTIP
                     ENALLWYSSCSKEIDAMRISCPNSSKHMNEWTQLMSGTLKHSSVKSTNLLTTEWGQGC
                     YYNTQCPADVNGPCGHTVTGCVATSMAMIMKHWNYPEHGFGANSYQTNYYGVLSADFE
                     NTTYQWNEMPDILIEENVALSTLMYQCGVAVNMQYTATSSGAAPGPSNFTAHFGYSPN
                     AHVVYRSDYSDEEWVALMKWEIDNNRPVQYVGDSDVFPDGHAWVCDGYDENDYLHFNW
                     GWDNLGGFYEPGTGIFPLNQYATIGIMPAAQCDIAVTELLAPYSQTFTEPAYITVKIE
                     NFSLEPISDIPVSYTVNNGIPVAEIVEQVIEPMSSVLYTFYTPCDFSQEQGSIYNVSV
                     FSSLACDAYLYNDTLHSGIESVMCAETPYVMGFTADESSAGWHSEDANNDGNTWQIPA
                     YNPAIENDYAFYQCNGVQADDWLFTKCLILGVNKMYRLSFDYKATGMYWPQNLEVRLG
                     TAQSNEDMYLVLDSIVGFNNSQPETCVVYFTADEHPHHYIGFHSFSEPDMLAMTIDNI
                     VVEEMAEPDVAVVDIISPAESCNMSTEPVTVKIRNLSSATLSQLPLSYILNNGAVVQN
                     IVDVSLLPGETYTYTFEEPVDMSAYGAFNLKVYSSYATDLNHANDTLIRDYQNIEAGE
                     IPYINGFDDEESLEGYVIENTNNDNRYWQYYPSAGHQNLGCMRYEYSDFNAADDWFFT
                     RCLFLEPEYIYTLSFYQKVESSQWHESMEVYFGQNPTSAFMNSILLNLPDLSNELWQD
                     EHTDFSVPEEGFYYIGFHCNSGAQMFNLYIDDIQVDGEINHANTWESISISVFPNPAS
                     ERVYIRIPEIAEYNISIYDVQGRVVLKNQGYSEIINVDTENLNSGIYMIEIRTNRQVY
                     NSKLVVR"
     gene            complement(8984..>11513)
                     /locus_tag="PLK75_09500"
     CDS             complement(8984..>11513)
                     /locus_tag="PLK75_09500"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOY39521.1"
                     /translation="VTGVLHMGHMLNNTIQDILVRRARQLGKNSVWIPGTDHASIATE
                     AKVVAKLKSEGLSKNDLSRDEFLKHAWEWKEKHGGIILEQLKKLGASCDWDRTCFTMD
                     EARSESVLRVFVDLYNKGLIYRGVRMVNWDPSAQTAVSDEEVIYTDEQSKLYYVRYPI
                     EGSSDFIMVATTRPETILGDTAVCVNPADPRYKHLHGKHVIVPMVNRIVPVISDSYVD
                     MEFGTGCLKITPAHDMNDYEIGRKHRLESIDIFNPDGTLSEAAGLFVGHDRFEARKMA
                     EDLLQQLGALHKVEDYNNKIGRSERTGSVIEPKISTQWFLSMEKLAQPALKAVMNDDI
                     RVYPPKFKNLYRHWMENVRDWCISRQLWWGHRIPAWYLPGGNEYIVALNETEALKAAR
                     EKSGNTSLQLSDLRQDEDVLDTWFSSWLWPISVFDGIRYPDNPDIRYYYPTNDLITAP
                     EILFFWVARMIIAGYEYRNEKPFKNVYLTGIVRDGLGRKMSKSLGNSPDPLELIEKYS
                     ADGVRVGMLLCSAAGNDLLFDIKKDEKTGEENYPLPEQGRNFANKIWNAFRLVKSWSV
                     DENEPQPDAAKAAGEWFTHRFAQELEQLNQQFDSFRLSEALMTVYKLFWDDFSAWYLE
                     LVKPAYGKGIDKDTYNSTIAHFENMLKLLHPFMPFITEEIWQQLGSRKQGESIMIEMW
                     PNTSEFSLQLVDQFDYARELVTAIRNIRQSKNIPQREQLRLIRNESNALTKQMIPVVE
                     KLAGLSEIKENGVKPEGTATILVKNDEFYLELGNMINKDEELAKLKAELEYTEGFLNS
                     VLRKLHNEKFVSGAPEAVVAAERKKQADAEAKIAILKGQIDSLN"
BASE COUNT         3508 a         2481 c         2200 g         3324 t
ORIGIN      
        1 acgacaccga aattatgaac acctgtagca tcaagtccat ttaacccgga ataagaatac
       61 gttccgtcgc agaggtactc atcagcaaga ccaccatcaa aataaacatc agcactgtac
      121 catccgctgc tgcaacctgg gagtccacta attgtatatt caatattagt aatgactgaa
      181 ttcgtttgcc cgcaattgaa ataagtgatt gcatacgact ggtcacagtc ggttcctgag
      241 taattatagg tactcgtata ggaataattc aatgcggaag gcacggaaac gtcgagttgg
      301 gcagagctgc cagaacaaat tgttgtaccc ccggtagccg tcaccgaaaa gccagaacag
      361 ccgctggaag ccagaaaaga agtaagctcc ggttcaaaat caggagaata atttttcatg
      421 tcgcccttgt cgcgattaat ttgaccggaa atatgacttt caatatttgg agaaggagca
      481 tcatcaccat cgggaaaaga attgtaagca tcctgctcaa aatagcaggc tattggtgca
      541 tccgacaata ccgattgcag ctgtgctaca gctgttgagt gcattacgat gcacattgca
      601 gtaatgagaa tgagtacagt atttttcatt ttattattat tttcattcta aataatcgca
      661 taaattaggg tcataaatcg caaataattc tgactacgat gtgcaaaacc tacattttta
      721 atcaactgac aaacacttgt aattagaata caaatatata agtattctta gaattagacg
      781 caaaaatata taaaagttgc taacaatttt acaaaaaaaa atcaggcgat ttatttagga
      841 ttatttacac tgtatttgcg agaagattat ttataaggaa agcgattcca ttatcggtat
      901 ttgatggtat aacagtatat atttgtttca atgcttccga tccattttcc acaacaaaag
      961 aatttaccga ccattccagc atatcgctat cgttgaaatc attacccact acgtgaacat
     1021 tcttactttc aattgacagc tcattacaaa tctcggtaat accatgagct tttgaaacat
     1081 cgtaaggaaa gatttcgacc cacaaactta ttccgtcgaa aggcgaagta gtcctgatta
     1141 ctttaaaatt gttaaaagca ctttgaatat gttgaaaatg accttcgtca tcattacaga
     1201 taacgacaaa ttgtgatgcc aattcaggtg ggtcaactag aagtttcaca tcaagtctca
     1261 tgtaatgcgc aagtctcctt tggaaatcat catgttcgta ttctgcagta taagcataaa
     1321 accgatgatt atccggcggg gctttgtgaa gcatgaaatt atacccgttc tgcgataacc
     1381 atttcattat taatttcgtg tcgctttttt caagcgtcga tttacttcga atttttttgg
     1441 ttttcaaatt gattattcca agtcctgagg aaaaaatcac ataatcgacc ggaaatgtaa
     1501 aatcgattac ctgaaacaac gaaaacaacg aacgacctgt tgcaattact cttacgatac
     1561 ctttttctcc gagtcgctcc aatgcatcaa tgttcgccaa tgaaacaaag ccggaggtac
     1621 ttgcaagagt tccgtcgtaa tcggtaacaa acattttgca tggcaaatga taatgcaata
     1681 tcaaccggtt tatttgtgtt tattcaaatt tactatatga agcaattggt catcgatgaa
     1741 taattcttca taatcgcgga tcataccggt aaagaatttt ccaggcttct gttcgatata
     1801 taatagcatt aacccgagaa attctttccc aaaccatatt ggcaccacta cccgatgctc
     1861 atttattccc tgtatttttt gagtaccttc gtaacgaatg ttttcgtttt gagcagcata
     1921 ctgccataaa tcatcaacat tttgcagatc ttctccagct aatttatccg acacatagat
     1981 atttcgctta tcaccaatta taaaaacaga atgatgcaca tcgaaaaaca taaaactgaa
     2041 gaaactcaca gtaaaccggt tcacgtgggt agcaaactgc tcaaccgtat gaatttcttc
     2101 cgaagcaatt ttattgcgta tatcatcgaa agcgtcaacg agtttaaggg ggatatgaga
     2161 aataacatac agataagctc cggcaatggt acaaataaca acaaaaagaa aaggtatcaa
     2221 tatcagcact gccatattgg aaacacccat tttttgaagt atcagaatga taggataaat
     2281 gctcatgcag cagaatagta cataaaaaac tatcagtatc aaacgctggt aatgaaatat
     2341 tttttgataa gtacccatag ttgttgaaat tttttacaag taaaattaaa atttatttaa
     2401 gatttttgta cgaccatcta aaatattatt tttacaaaca gaaaattata caatatggat
     2461 attacaggaa aaatcaccca tatactacca gtacagacgg gtgaagggaa aaacggtaca
     2521 tggaaaaaac aattatttgt tattgaaacc acagatgaat ttccgaaatc ggtatgtttc
     2581 acagcctggg gcgatcgggt tgaacttagt aagatgtcgg ttggggaaca aataaaggtg
     2641 tatttcgacc tcgaaagccg tgagtataac aataagtggt tcaccgacgc caaagcatgg
     2701 aaagccgaaa gtatcggtgc tgcaaacggc actgttactc cgactgcata cgatcaaccg
     2761 gaagccataa aaaaccttga acccgaagag ggtgatgatt taccatttta gtaatccgta
     2821 atttattaac tctttacaat cccagtcgct ttcttgctat cagcaagtta ttctggtttt
     2881 gaacatcgtt tggattgagt ttatatgctt tttcgaaatg cataagtgcc aggctgtcgt
     2941 tttcctcaat accgtaggaa acaccaatat agcggtgaca ttctgcatcg ttcgggttga
     3001 tagaaacagc tttgagaagc gtttcacggg caagattaaa tctgttcagt gagccatata
     3061 caattccaag cgatttcagt atatcgaaat tacccggatt catagagttt gctttttcga
     3121 gatatattac tgcggaatcg ggacgattct ctgccagtcg gtattgctct cccatatacc
     3181 agaacgactc aaaccggttg ggtgctactt tgctaacttc tgtccaaaag gcaatctcaa
     3241 attttggatc agtaacattg ttctgcagaa cgccgatggc atttccccac acgtcggtat
     3301 tgtttggatc ggcccgcaat gctttaaggt aataatcaaa catcgtttta taatcgccat
     3361 acattatgta atagcaattc ccaaggcgaa ttaatggttc tgtgtaggat gggtatactt
     3421 caattgcttt tctgaaataa ggcattgctg atttcagtat cttattgcgc tccgctgtat
     3481 ccgtctgtgt ttcagccaag ctatacaatt cgctggcata cgaactgttg cctttggcgc
     3541 tatttaccga aacagcaaca tcatggccaa atagagtaag gttatctttc cagtcaaaat
     3601 ttcttattat ggtttttccg gaaaatgcaa gacagataag caacaacgta ccaattgcaa
     3661 ctcgctgcaa gagctttgtt tttaccatcc ttgtatttac aaaatacgca atcgcaaggc
     3721 agaaacctaa gagagacatg tataaaaatc gctcgctcat gaaagcaccc actgtaaaaa
     3781 acaagttcga tgttattgaa agtgtagcaa gataaaccca gactgcaaat gcatagggcg
     3841 atttacgcaa tgtaccaact atagcaacaa cacccaacgc cacatacaac aacagcggca
     3901 aaattgcccg ccagtcggac cattcaatca agggaatgtg atagggataa taatcccagg
     3961 taagcgggtg gggaaaaacg agcagcttca gatagatacc aagtgtcata aatattgttg
     4021 cgtacttctc tcctgtcgtg gcttcgagaa acggattgtt catcagctca cccacttcgt
     4081 ttcccagtgt attacccagt acagcaaagc gggtaattaa gtaaataatg cttgctcccg
     4141 caattgaaaa aaacgatgtc aaatattgct tcctgtttgg tttcctgaaa aaatacatac
     4201 ttacaggaat aataaataaa aaagtgattg ctgtttcctt actgaaaaca gccagtaaaa
     4261 accaaataaa agatattgcg atatgccctt tgctttttgt atcggtgtac ttcaatatcg
     4321 aatcccagga aagcaataca aacaagaagc aaaaaatttc atcgcgtcct ttaatgtttg
     4381 ctaccgcatc ggtatgcaac gggtgcacag caaacagcag agatgcaata aatgctatac
     4441 tgaaatacca tttggtttta ttattatcct tacataaacg caataacaac ctatacagca
     4501 ctataaccga caaggcataa aaaagcaaat taaaaaaatg gctcacatgc ggatttccac
     4561 caaaaatctc ctgctcaaca gcaaagctta tcatagaaag cgggcgatat cttccgcctg
     4621 caacagcttg attattatcg ccaaacatac cggccatggc gtcttttgta agtatgtcgc
     4681 cgattcctgc aaaacctttc tgcgtatagt tatttttaaa aatgatcatt ttgtcgtcga
     4741 gggcgtacct gttggggatc gtattcaaat acagcattac acttaatccg aataagatat
     4801 aatatggaag cctgttcttc ttcatagcat tttcattttg gatataaaac tacaataaaa
     4861 tcagatttat ttcagattcg aaattctatt ttttttattt ctgcatcatg caataatatt
     4921 ttgaaaaaat cgagaataag ctccaaaaac tatatatttg ttgaataatt taaactaata
     4981 aaaccatgga ttacgaagta attttacaaa acatctggtg gataatactt atcctcatca
     5041 ttctgttttc aggactaaaa acagttagtc agggaacaat tgctgtaatc acgctatttg
     5101 gcaaatacag gcgcattatg cgacccggac tgaatttcaa aattcctgtt atcgaaaaaa
     5161 tagcccgtcg tatcagtatt cagaaccgct cagttgaact ggaatttcag gctgttacac
     5221 aagatcaggc caatgtatat tttaaaagta tgctgctcta tgctgttcag aacgaaaatg
     5281 aggaaacaat cgtgaaggtt gcttttaagt tttattccga taaagacctg atgcaagcgc
     5341 taatccgcac catcgagggg aatatccgtt cctatgtagc tactaaaaag caagctgaga
     5401 tacttgccct tcgcaaggaa attgtcgaca gcgtaaaact ggaagtggac catgtactcg
     5461 aagactgggg ctatcatctt ctggaccttc agataaacga tatcactttt gatgctgcca
     5521 ttatggaaag tatgagcaaa gttgttgcca gcaacaacct gaaagccgct gccgaaaacg
     5581 aaggacaggc attattgatt accaaaacca aagccgctga agccgaaggg aatgctataa
     5641 aaatttctgc tcaagccgaa atgcaggctg cgcaattgcg cggacaaggc gtcgcattat
     5701 tccgtcagga agtggcaaaa ggtatgagtg aggcagcgga gcaaatgaaa caggcaaacc
     5761 tcgataccaa tgtgatatta ttcagtatgt ggacagaggc tattaaaaac tttgccgagt
     5821 acggtaaggg gaatattatc ttcctcgatg gcagtagcga aggtatgcag catacaatga
     5881 aacaaattat gggactgatg aagatggatg aaggtaacca gggtaaaaat cactagattc
     5941 ctcagtcact gagcgctgac tttaacgcac tacaagtttt gaattataaa cttgccggtt
     6001 tgttcgtatt tctatcatat aaattccgga attcaggttc tccgtatcaa cattgatgat
     6061 ctcggagtat ccttgattct ttaaaacgac cctgccctga acatcataaa tacttatatt
     6121 gtattctgca atttcgggaa tcctaatata aacacgctcg cttgcagggt taggaaaaac
     6181 agatatggag atgctttccc atgtatttgc atgatttatt tcaccatcaa cctgaatgtc
     6241 gtcgatgtaa aggttgaaca tttgcgcccc actattacaa tggaatccga tataataaaa
     6301 cccttcttca ggaacactaa aatcagtgtg ttcatcctgc cataattcat tagatagatc
     6361 tggtaaattg agtaaaatgg aattcataaa agcggaggtt ggattttgtc cgaaatacac
     6421 ttccatactc tcgtgccact gacttgattc aactttttgg taaaatgaaa gagtataaat
     6481 atattcgggc tccagaaaaa ggcagcgtgt aaagaaccaa tcgtcagcag cattaaaatc
     6541 gctgtattcg taacgcatac aaccaagatt ctgatgccct gctgatggat aatactgcca
     6601 ataccggtta tcgttattcg tattttcaat tacatatccc tcaaggcttt cctcatcatc
     6661 aaacccatta atataaggta tctctcccgc ctcaatattt tgataatcac gaatcaatgt
     6721 atcattagca tgattcaggt ctgttgcata ggaagaatat actttcagat taaatgcacc
     6781 ataagccgac atatcaacgg gttcctcaaa agtataggta taggtttctc ccggcaataa
     6841 acttacgtca actatatttt gcaccaccgc tccgttgttc aaaatgtacg acaaggggag
     6901 ctggctaagc gtggcagaac tcaggttcct gatttttaca gttaccggtt cagtgctcat
     6961 attgcaggat tctgcaggtg atattatatc cactacggca acatcgggtt cagccatttc
     7021 ttcaacaaca atattatcaa tcgtcattgc aagcatatcg ggttcgctga aagaatgaaa
     7081 accaatgtaa tgatgcgggt gctcatctgc tgtaaaatat actacacaag tttccggctg
     7141 tgaattatta aaacctacaa ttgaatcaag tactagatac atatcttcat tcgattgcgc
     7201 cgtaccaagc cgtacttcaa gattctgagg ccagtacata ccggttgctt tataatcgaa
     7261 gctaaggcga tacattttat tcacaccgag tatcaggcat tttgtaaaaa gccaatcatc
     7321 ggcctgaaca ccattacact ggtaaaatgc gtaatcattt tcaatcgcgg gattatatgc
     7381 tgggatctgc caggtattgc catcgttgtt cgcatcttcg gaatgccagc ctgctgaact
     7441 ctcatcggca gtaaagccca tcacataggg tgtttccgca cacataacac tttcgatgcc
     7501 gctatgtagt gtatcattgt aaagatatgc atcgcaggca agtgaactga acacgctcac
     7561 attatatatg cttccctgtt cctgactgaa atcgcatggc gtataaaagg tatagagcac
     7621 cgacgacata ggctcaatga cttgttcaac tatctctgct acaggaattc cgttgtttac
     7681 ggtataagaa accgggatat ctgaaatcgg ttctaagcta aagttctcga tcttcaccgt
     7741 gatataagca ggttcagtga atgtttgcga atagggtgcc aacaactcag taaccgctat
     7801 atcgcattga gcagcgggca taattccgat agttgcatac tgattcagtg ggaatattcc
     7861 tgttccgggc tcgtaaaagc cacccagatt gtcccatccc cagttaaaat gcaaataatc
     7921 gttttcatcg tatccgtcgc atacccaggc atggccatcg ggaaacacat ctgagtcacc
     7981 aacatattga accgggcggt tgttatcaat ttcccatttc attaatgcaa cccattcctc
     8041 atcgctgtag tctgaccgat aaacgacatg tgcattgggt gaatacccga aatgcgcagt
     8101 aaaattcgat ggtcccggag cagctcccga actggttgcc gtgtactgca tgttaaccgc
     8161 aaccccacac tggtacatca gcgttgacaa agccacgttt tcttcgatga gaatatcagg
     8221 catttcattc cattgatatg tagtgttttc gaaatcagct gagagcaccc cgtaataatt
     8281 tgtctgatac gaatttgctc caaacccatg ttccggataa ttccagtgtt tcattatcat
     8341 tgccatcgaa gtagccacac atccggtaac cgtatgcccg caggggccat tgacatcggc
     8401 tgggcactgg gtattgtaat aacagccctg cccccactca gtcgtaagga gattggtcga
     8461 ttttacggaa ctatgcttga gcgttccact cattaattgt gtccattcat tcatatgttt
     8521 cgaactattt ggacatgaaa tcctcatcgc atctatttct ttcgagcagc tactatacca
     8581 taataaagca ttttcaggaa ttgtattcag gtcatggaaa ccttctcctg aataggcaat
     8641 tatcggggca acacccttat acatcgaaag tgcaacaaag ccacccgatt gaaaacagaa
     8701 aattgcgatg ttttcgctat ttcccgtacg aataaacaac tcatccgaaa ttaaagaatc
     8761 attgcattgt ttgttaattt ccttgtaaaa caatatggca agattgcgga cttcatccct
     8821 actcaggttt tgagaaaaca acgacaacgg catcaataca atcgatagcc acagcaagtg
     8881 taatattatc tttttgagca ttgtctgaaa atattaaata atacaagcaa attcattgca
     8941 ttttaacata tgcaatttac taaaaattat cgaaattaaa ccattagttc agactatcaa
     9001 tttgtccttt cagtatagct attttagctt cagcatctgc ctgcttcttg cgttctgcgg
     9061 caaccactgc ttcgggagcc ccacttacaa atttttcgtt atgcaatttg cgcagtacag
     9121 aattgagaaa accttccgtg tattcgagtt cggctttcag cttggcaagt tcttcgtctt
     9181 tattaatcat gtttcccaac tccagataaa actcatcgtt cttcaccaga attgttgcag
     9241 ttccttcggg tttaacccca ttctctttga tttcggacaa cccggcaagc ttttctacca
     9301 ccggaatcat ttgttttgtt aatgcattgc tttcgttacg gataagcctg agttgttcac
     9361 gctggggaat atttttgctt tgtctgatat tgcgtatggc tgtcaccagt tcccgggcat
     9421 aatcaaattg gtcaacaagt tgtagactaa actcggatgt gttgggccac atttctatca
     9481 taatgctttc gccttgtttt cgacttccta attgctgcca gatttcttca gtgataaaag
     9541 gcataaacgg atggagtagt ttaagcatat tttcgaaatg tgctatggta gaattataag
     9601 tgtctttatc aattcctttt ccataagccg gttttaccaa ttcaagatac catgccgaga
     9661 agtcatccca aaacagtttg tacaccgtca tcagagcttc ggaaagacga aaagaatcaa
     9721 attgttgatt cagttgttcc agttcctgcg caaagcggtg ggtgaaccat tctccggctg
     9781 cctttgcggc atcgggttgg ggttcgtttt cgtcaacgct ccacgacttc accaatctga
     9841 aggcattcca tattttattg gcaaaatttc ttccctgttc gggcaaagga tagttttcct
     9901 cgccggtttt ttcgtctttc tttatatcga aaagcaagtc gttaccggca gcagagcaaa
     9961 gcaacatccc tacccttacc ccatccgcac tgtatttttc tatcagctca agtgggtcgg
    10021 gtgaatttcc cagcgatttc gacatctttc gtccgaggcc atcgcgaaca atacctgtga
    10081 ggtaaacatt tttaaatggc ttctcgttgc gatattcata accagcaata atcattcggg
    10141 caacccagaa aaacagaatt tccggagcag taatcaaatc gtttgtagga tagtaatacc
    10201 gtatatctgg gttatcggga taacgtattc cgtcgaatac cgaaattggc catagccaag
    10261 agctgaacca tgtatcaagc acatcctcgt cctgccgtag gtcgctaagt tgcaaagaag
    10321 tattgcccga tttctcccgg gcagctttca gggcttcggt ttcgttaaga gccactatat
    10381 attcgttacc cccgggcaga taccatgcag gaatacgatg cccccaccaa agctggcgac
    10441 tgatgcacca atcgcggaca ttttccatcc aatgacggta caggttttta aacttgggtg
    10501 ggtacaccct tatgtcgtcg ttcatcacag cttttaacgc cggttgagcg agtttttcca
    10561 tactcaggaa ccactgtgtc gaaattttgg gttcaatcac cgaaccggtt cgttcagagc
    10621 gaccaatctt attattgtag tcttcaactt tatgcaatgc accaagctgt tgaaggagat
    10681 cctctgccat ctttcgcgct tcaaaacgat cgtgaccaac aaacaatccg gcagcttccg
    10741 ataaagttcc atccgggttg aaaatgtcga tgctttcgag cctgtgctta cgaccaatct
    10801 cgtagtcgtt catatcgtgg gcaggcgtaa ttttcagaca tccggttccg aattccatat
    10861 ctacataact gtccgaaatc acaggtacta ttcgattaac catagggaca atcacgtgtt
    10921 ttccatgtaa gtgcttatag cgaggatcag cagggttcac gcaaaccgcc gtatctccca
    10981 aaatcgtttc gggacgggtg gttgcaacca taataaaatc gctgctgccc tcaatgggat
    11041 aacgaacata atataattta ctttgctcat cggtgtaaat aacttcttcg tccgacactg
    11101 ctgtctgcgc tgaagggtcc cagtttacca tccgcacacc tctgtaaatc agccctttgt
    11161 tgtagagatc gacaaacaca cgcagtacac tttcggaacg agcctcatcc atggtaaagc
    11221 aagtcctgtc ccaatcgcat gaagcgccca gctttttcaa ctgttcaaga ataataccgc
    11281 catgtttttc tttccattcc cacgcatgtt tcaggaactc atcacgactc aggtcgtttt
    11341 tcgaaagacc ttcgcttttt agttttgcaa ccactttcgc ttcggtagca atcgaagcat
    11401 ggtcggtacc aggtatccat actgagtttt tacccaattg tcgcgcacgc cgaaccaata
    11461 tatcctgaat ggtattgttg agcatatgac ccatgtgcag cactccggta aca
//
LOCUS       DAOOQP010000093        11401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7084_length_11401_cov_4.967742, whole genome shotgun sequence.
ACCESSION   DAOOQP010000093 DAOOQP010000000
VERSION     DAOOQP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7084_length_11401_cov_4.967742"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2249)
                     /gene="amrS"
                     /locus_tag="PLK75_09505"
     CDS             complement(<1..2249)
                     /gene="amrS"
                     /locus_tag="PLK75_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOY39522.1"
                     /translation="MKKGVRLVIILSSWLIIMLCATSLNSFSQNTELFMKQNRKPAVA
                     GTFYSDDPETLLSDMKAFFNNAKPRSCIAVRAIISPHAGYVFSGQTAADAFNQIDENI
                     SYSRVFVICSSHQMYFNGASVYNVGNYETPLGEIEVDTALCSKLIQENILFKFVQGAH
                     SKEHSLEVQLPFLQYKLKSPFLLVPIVIGSNDTSEIQLIARALQPYFTPENLFVFSSD
                     FSHYPGYEDAVKTDAETADAICSGKPSVFLNTLSKHKKSGIRNLVTDLCGWSSVLTLL
                     YMADGKQLTYNKISYTNSGDSKFGEPGRVVGYNAIAVSDENKTFSLSDSEKKTLLQIA
                     RNTAYNKLAFGKSYNVDSLLITDALQSECGVFVSLHKNGELRGCLGRFTANEPLYKLI
                     GEMAVASCLNDSRFSPVTASELDSLEIEISVLTPMRKITDTSEIVLGKHGVYIRSGMY
                     SGTFLPQVATQTGWTLEEFLGHCSRDKAGIGWNGWKQAELYIYEALVFNESMFSKEHT
                     SDNHTLFYEVLENGKVKCTLCPNYCELSNGQTGLCNARRAENNSMKALTYGKLAAVHN
                     DPIEKKPLYHFLPQSMTYSIGTGGCNLHCKNCQNHHISQVSPEEVDYIEATPEQVVEN
                     ALKRKSASISYTYNEPTVFYEFMLETAKLAREKGLKNIIVSNGYISEEALRKLIPYID
                     AANIDLKCFNDSVYQKMCSAHLEPVLNTLKVLRQSGVWLEITNLIIPGWSDNMVEIEQ
                     MCKWLVENG"
     gene            2404..3999
                     /locus_tag="PLK75_09510"
     CDS             2404..3999
                     /locus_tag="PLK75_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY39523.1"
                     /translation="MKRFLFFLILLIAFSLSLKSQVFDEQLHKFGRVINLINTMYVDS
                     VESSKIVETAIIEMLKELDPHSVYISKEEVARMNEPLEGSFEGIGIQFRIDKDTLIVS
                     GVITGGPSEKVGLEAGDRIIQVEGNTIAGIGLQNDSVIKLLRGDKGTVVNIVVKRPGT
                     LKLLDFSITRDKIPLYSVDAGYVSKEGIGYIKLNKFAKTTMDELNEVFNLFQKAKVKG
                     IILDLSDNTGGYLDQSITLVDEFLKENQMIVYTEGLHSPKQEYKSTSKGRFDKCKLVV
                     IVDEGSASASEITSGAIQDWDRGIIIGRRTFGKGLVQRPYSLPDGSMIRLTVARYYTP
                     SGRLIQKPYTDDLDEYSSDIVNRYKHGELSNQDSIVFADSLRYETLINKRSVYGGGGI
                     MPDFFIPLDTTMYGEFHYALLRKGLLFRFINSYIDQHRSQLKSDYPDIETFESKFHVD
                     KNVLQELYKFASDNKISIDSFPAATEVQQYRLQVHLKSLIAGELWKTNEFYRIFNEIN
                     PNFVEARNILLSDDRYNTLLKNK"
     gene            4004..4594
                     /gene="pnuC"
                     /locus_tag="PLK75_09515"
     CDS             4004..4594
                     /gene="pnuC"
                     /locus_tag="PLK75_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOY39524.1"
                     /translation="MSETLEWIRLHWVELAGACLGLWSIYLQILRKPLYWPVSIVMVI
                     LYIFVYFHARIYADMSFQFYYLAISIYGWYYWITHKRAEKETVESDISRITRLTFWSI
                     VIFCTVVFVALGLVLSEFTDSDVPWCDALTTSLSFAATWLLARKVLENWLFWIVVDSV
                     SVAIYIYKGLYPTAFLFTVLTALAFVGYFKWKKEIH"
     gene            4591..5097
                     /locus_tag="PLK75_09520"
     CDS             4591..5097
                     /locus_tag="PLK75_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY39525.1"
                     /translation="MKRIVLTGPESTGKSQLCLKLAQHFDCPMIPELARDYLLLHGAN
                     YTESDVLKIAEMQIEAEEQVSSSKHDFLFIDTDLIITKVWLQHCYKRVPDWINQCIDN
                     TPRHLHLLCYYDLPWIQDPLRENPSIRKELFEAYLNEIRFFGFRYSIIKGNGDTRFNN
                     ALKAISLL"
     gene            complement(5094..5957)
                     /locus_tag="PLK75_09525"
     CDS             complement(5094..5957)
                     /locus_tag="PLK75_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39526.1"
                     /translation="MFLFADSGSTKTEWMLVRGNGDVVAHKKTMGLNPYFVTTGDVSA
                     AVNSVIPADVDPLEVQHVFFYGAGCGNSERRQMMVDSLENTFTVAIVDVYTDILGAAR
                     AVWQKERGIAIILGTGANACAYNGDNIFRKVMSLGYLLGDEGSGAYLGKKFLDTYYKS
                     RFDHDFMRTIRNELDMDYNNVMNSLYRGSTPSVYLASFVPFIHKYREQAQLAVMLKDA
                     FQDFFDNIVRMIPECCDLPVGFVGSIGYHFEKEIRTVAADNKILVGQFIQDPLEGIVK
                     FHLSDEVALVY"
     gene            6072..6809
                     /locus_tag="PLK75_09530"
     CDS             6072..6809
                     /locus_tag="PLK75_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOY39527.1"
                     /translation="MKAMILAAGLGTRLKPLTDNKPKALVEINGVPMLGLLIEKLKRY
                     GCDNIVVNIHHFGQQIIDYLSQNNNFNISIAVSDERFMLLDTGGGIKHARKLLPESEA
                     FIVHNVDIYSDIDIAAMYRYHLSSGNLVTLAVKQRESSNYLYFDSNNLLCGWKSYKTG
                     REIISRPIEQYKELAFSGIHIINPSIFDMITEEGCFGIVPVYLRLAATHSIGAYLHQG
                     DTIIDLGKTESLLEVERAILQKNDDLQ"
     gene            complement(6868..7947)
                     /locus_tag="PLK75_09535"
     CDS             complement(6868..7947)
                     /locus_tag="PLK75_09535"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOY39528.1"
                     /translation="MKKCLLLLLVVAVAYVTNAQNRLVVFSDDGEKFLLFMNGEQQNQ
                     MPEVNVKSKDLNSDSYRVKIAFNNKSLPELNKSITFLEPNKEMTVSIVKNKKGEYVLR
                     LISEAPYTALPASETQTSVKEVVYETPETTPPTTQTVTQVTTTDINTQTTTSGGNTQT
                     VGATLNVNSNGVSMQVTDPDGENVQLNVNINVPDAPATSTTTVTTTTSTTYNESVTIT
                     ETDQQHYETPDQPAERPHDHHGNQHGEHQHGYDPLPPQQYNDGGCRYAMSDADFLSAS
                     KSIEEKSFSDSQLTLAKQIAKSNCMTCDQIKKVMEIFDYESTRLEFAKYAYDFAYDKN
                     NYYKVNDAFQFESTIEELNEFLEGK"
     gene            complement(8007..8960)
                     /locus_tag="PLK75_09540"
     CDS             complement(8007..8960)
                     /locus_tag="PLK75_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOY39529.1"
                     /translation="MKKLLLFTAVVCMATPVFSQGSGNYYFSQSNNPNFKKEYKDYDY
                     EMNQQNYYNANYSNYNSSPTYSKGSDTVCYVIANVMMNVQPDYFVIMLGTSQVGESLE
                     VCHQLLDQRINDFITSLGAEGIQQKDVYVDFISQAPIFSYEEEKTLFSKKYVKVPKGF
                     ELKKNIHIRYNDRKQADKIIKNAAASEIYDIIKVDYIVNDMQSILDTMRKDCIDILNK
                     KTQEYNTLGVNAANMYRTYEESHYCYYPLQRYETFISYTPDNLKALSSTASLINENSK
                     INLYYSKLPDNYFDKVINPDVLEPVIQYTYTVKMKTVFKKN"
     gene            complement(9053..9256)
                     /gene="xseB"
                     /locus_tag="PLK75_09545"
     CDS             complement(9053..9256)
                     /gene="xseB"
                     /locus_tag="PLK75_09545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOY39530.1"
                     /translation="MGKEKISYQQALAELEQIVEKIEQQEIDVDELADNVRRATYLIN
                     TCKAKLRSTEDELNQILSDFTKD"
     gene            complement(9267..9608)
                     /locus_tag="PLK75_09550"
     CDS             complement(9267..9608)
                     /locus_tag="PLK75_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOY39531.1"
                     /translation="MDNNWKSRQGVVYSTNPEFTFSHGEAPESELLPPEKQQLYIAID
                     RKQRNGKTVTLITGFSGPEDALLELAKSLKQYCATGGSAKDGEIYIQGEFREKIKNYL
                     LSKKYKVKLKG"
     gene            complement(9733..10428)
                     /gene="ispD"
                     /locus_tag="PLK75_09555"
     CDS             complement(9733..10428)
                     /gene="ispD"
                     /locus_tag="PLK75_09555"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOY39532.1"
                     /translation="MKTNIAIIVSGGSGVRMHSDIPKQYISVAGKTILEHCVAKFIHH
                     PLISGIIVVAHTDYIDTVEELMNDFRKIKHVEIVCGGAQRYHSVYQGLLKAEAMAPDG
                     NVLIHDAVRPNVDETTINGVVEALSEYEAACPLIAVADTVMQLKEGFQPKPLNRSEIK
                     LVQTPQGFRLDKILFAYREFFSQSNIEPTDDASIYLRYAFNADLAVTKGNPENFKITF
                     PGDISRFEQLFSV"
     gene            complement(10583..10963)
                     /gene="gcvH"
                     /locus_tag="PLK75_09560"
     CDS             complement(10583..10963)
                     /gene="gcvH"
                     /locus_tag="PLK75_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOY39533.1"
                     /translation="MNIPKNLLYTKTHEWIRVEGDEAVIGITEFAAEQLGEIAFVEIE
                     TEGEELAKGEVFGSIEAVKTASDSYMPVAGEVLEANELVKNDSTLVNSDSFGDGWLIR
                     IKISDPAELKDLLTPADYEALIKA"
     gene            complement(11023..>11401)
                     /locus_tag="PLK75_09565"
     CDS             complement(11023..>11401)
                     /locus_tag="PLK75_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39534.1"
                     /translation="SFTNNQLTENLTDEFVIGSGYRFKDVEITIKTGGKQRNFKSDLN
                     LRVDFSYRNSLTIIRKLNEEVDQPTAGQRVFTIKTSADYVLSDRFNLRIFYDQVINRP
                     RISLSYPNSNTNFGVSLRFTLAS"
BASE COUNT         3221 a         2399 c         2317 g         3464 t
ORIGIN      
        1 aacccatttt caaccagcca tttacacatt tgctctattt caaccatatt atcggaccaa
       61 ccgggaataa tgaggtttgt gatttcgagc caaaccccag attgcctgag tactttgagt
      121 gtattcaata ccggctccag atgggcagag cacatctttt gatacacaga atcgttaaaa
      181 catttgagat cgatgtttgc tgcatcaata tacggaataa gtttccgaag ggcttcttca
      241 gaaatatacc catttgaaac tataatattt ttaaggcctt tttcacgtgc cagctttgct
      301 gtttcaagca taaattcgta gaacacagtt ggttcgttgt acgtatagct tatggaggct
      361 gatttacgtt tcaatgcatt ttcgaccacc tgttcaggag tggcttcaat ataatcgacc
      421 tcttcgggtg atacctgcga aatatgatga ttctggcagt ttttgcaatg caaattacac
      481 cctcccgtgc caatggagta tgtcatcgac tggggcaaaa aatggtataa tggtttcttt
      541 tctatcggat cgttatgaac tgccgcaagt tttccgtaag taagtgcttt catcgagtta
      601 ttttcagccc ttcgggcatt gcatagtccc gtctgaccat tgctcagctc acagtaattc
      661 ggacaaaggg tgcatttaac cttaccattt tcgagcactt cgtaaaacaa ggtatgatta
      721 tcgctggtat gctctttgct aaacatcgat tcattgaata ccagcgcttc gtaaatgtac
      781 aattcagctt gcttccagcc attccaacca attccggctt tatcgcgtga acagtgaccc
      841 agaaactcct ccagagtcca accggtttgc gttgctacct gaggcagaaa tgttcctgaa
      901 tacattccac tcctgatgta tacaccatgc tttccgagaa caatttcgct ggtgtctgtt
      961 attttacgca taggtgtaag caccgatatc tcaatttcaa gtgagtcaag ctccgaagca
     1021 gttaccggtg aaaacctcga atcgttcagg cacgaagcca ctgccatttc accaataagt
     1081 ttatacaagg gctcgtttgc agtgaaacga cccaggcagc cacgaagttc tccattttta
     1141 tgcaggctca caaaaacacc acattccgat tgcaatgcat cagtaatcag taagctatca
     1201 acattatatg atttaccgaa tgccagcttg ttataggcag tgttgcgagc aatttgaagt
     1261 aaggttttct tctcactgtc gctgagtgaa aaagtcttat tttcatcgga tacggcaatg
     1321 gcattgtaac ccactactct acccggctca ccaaatttcg aatcgcccga attggtatag
     1381 cttattttat tgtaagtcaa ctgcttacca tctgccatat aaagcaaagt tagcaccgac
     1441 gaccagccac acaggtcagt taccaggttt cttattccgg attttttgtg tttcgagaga
     1501 gtattgagaa atactgaagg cttccctgag caaatggcat cggcggtttc agcatcggtt
     1561 tttacagcat cttcatagcc tggataatgc gagaaatcgc tcgaaaacac aaaaaggttt
     1621 tccggagtaa aatacggctg caacgcacgg gcaattagct gaatctctga agtgtcgtta
     1681 cttccgatca cgattggtac aagtagaaat ggacttttca atttgtactg caaaaacgga
     1741 agctgaactt cgaggctgtg ttctttgctg tgagcaccct gcacgaactt aaacaaaata
     1801 ttttcctgga tgagcttgct gcataatgcg gtatctacct ctatttctcc aagtggcgtt
     1861 tcgtagttac ccacgttgta caccgaagca ccgttaaaat acatctggtg gctggagcaa
     1921 attacaaaaa cacgcgaata gcttatgttt tcatcaatct gattaaatgc atctgctgca
     1981 gtttgcccac tgaaaacata accggcatgt ggcgaaataa ttgcgcggac ggcgatgcat
     2041 gatcggggtt ttgcattgtt aaaaaatgct ttcatatccg ataaaagcgt ttcagggtca
     2101 tcggaataaa atgttccggc cacagcaggt tttcgatttt gtttcataaa caattcggta
     2161 ttttgagaaa acgaattcaa ggatgttgca caaagcataa tgattagcca cgacgacaaa
     2221 ataatcacca aacgaactcc tttcttcatt gggcacaaaa tttgaatatg attactcaat
     2281 ttataaaatt aatgcattcc gtcgtaaaat ggaaatattt ctttcattta ctaataaatt
     2341 gttaaaccgc tcatttttgg agaggatttc tttacatttg ttcaaaacat aaaaagccac
     2401 aggatgaaac gttttctctt ttttctaata cttcttattg ctttctctct atcgttgaaa
     2461 tcacaagtat ttgacgaaca attgcataag tttggaaggg taataaacct gattaatacc
     2521 atgtacgtcg acagtgtgga aagctcaaaa attgttgaaa cagcaattat cgaaatgttg
     2581 aaagagcttg acccccattc ggtttacatt agcaaagaag aagttgcaag gatgaatgag
     2641 ccccttgagg gcagtttcga agggattggt attcagtttc gcatcgataa ggacacattg
     2701 atagtttcgg gtgtcatcac cggtgggcct tccgaaaaag taggattgga agcaggcgac
     2761 cgtatcattc aggttgaagg aaacactata gcaggcattg gcctgcaaaa cgatagtgtt
     2821 attaaacttt tgagaggtga caaaggaacg gttgtgaaca ttgtcgttaa acgacccgga
     2881 actctcaagc ttcttgattt cagtataacc cgcgataaaa ttcctttata cagtgtagat
     2941 gcaggatatg tatcgaaaga aggcataggt tatataaagc tgaataaatt tgcaaagacc
     3001 acaatggacg aattgaatga ggtttttaac ctttttcaga aagccaaagt taaaggtata
     3061 atacttgacc tgagcgacaa cacaggaggt tatcttgacc aatcgataac actggttgat
     3121 gaattcctta aagaaaacca aatgattgtt tatactgaag gattacatag tccgaaacag
     3181 gaatataaat ccacgtcaaa aggccgtttc gataaatgca aattggtagt aattgttgat
     3241 gaaggctctg cttcggcaag cgaaatcacc tcgggagcaa ttcaagattg ggacaggggc
     3301 ataatcattg gtaggcgcac tttcggcaaa ggtttggttc aacgcccgta ttccctcccc
     3361 gacggctcaa tgatacggct taccgttgcc cgttattata caccatccgg cagacttatt
     3421 cagaagcctt acactgatga tctcgatgaa tattcatctg atattgtcaa ccgatacaaa
     3481 cacggggaat tatcgaatca ggacagtatt gtttttgcag attcgttgag atacgaaacc
     3541 cttatcaata aacgcagtgt gtatggaggc ggcggaataa tgcctgattt tttcattcca
     3601 ttagacacta ccatgtatgg agaattccac tacgctcttc ttcgtaaagg gctgttgttc
     3661 cgattcatta actcttatat tgaccaacac cgcagtcagc tgaaatcgga ttatcccgac
     3721 atagaaactt tcgagagtaa gtttcatgtt gataaaaacg tattgcagga actgtataaa
     3781 tttgcctctg acaacaaaat cagcatcgac agttttccgg cagctaccga agttcagcaa
     3841 taccggcttc aggtgcattt aaaatcgctg attgcgggtg agctttggaa aacaaacgaa
     3901 ttctacagga tcttcaatga aataaatcca aacttcgttg aagcccgtaa cattttgctt
     3961 tccgatgatc ggtacaacac gttgctgaag aacaaataac gcgatgtcag aaaccttgga
     4021 atggatcagg ctgcactggg tagagcttgc aggtgcatgc cttggtttat ggtcaattta
     4081 cctgcagata ctgcgaaaac ccctgtattg gcctgttagc atcgtcatgg ttatattata
     4141 tattttcgtg tattttcatg cccggatcta tgctgacatg tcgtttcagt tttactacct
     4201 tgcaataagt atttatggct ggtattactg gataacacac aaacgggccg aaaaagaaac
     4261 tgtagagagt gacataagcc gcattacaag actcacgttt tggagtatcg ttattttttg
     4321 tactgttgtt tttgtagccc tgggtcttgt tctatcggaa ttcaccgact cagacgtgcc
     4381 atggtgcgat gcattaacca catcgttgag ttttgcagca acctggcttc tcgctcgaaa
     4441 agtactcgaa aactggcttt tttggattgt ggtcgactct gtttcggttg caatatacat
     4501 atacaaagga ttatatccaa cggcgttttt attcacagta ctgacagcat tggcttttgt
     4561 aggttatttt aaatggaaaa aggaaattca ttgaaacgaa ttgttctcac cggacccgag
     4621 tcaacaggca aatctcagtt atgccttaag ttggcacagc attttgattg cccgatgatc
     4681 cccgagctcg cacgcgacta cctcttgctt catggtgcaa attacaccga gagcgatgta
     4741 ctgaaaattg ccgaaatgca gatcgaagct gaagaacaag tgtcttcttc aaaacacgat
     4801 tttcttttta tcgacaccga tcttatcatt acaaaagttt ggctgcagca ttgctataag
     4861 cgggttcccg actggataaa ccagtgcatt gacaacacac caagacatct tcatttattg
     4921 tgctactacg atctgccttg gatacaggac cctttgaggg agaatccctc gatacgcaaa
     4981 gaattgttcg aagcatacct taacgagata cgctttttcg gatttcgcta cagcattata
     5041 aaagggaacg gagatacccg gttcaataat gcactgaagg caataagcct gctttaataa
     5101 acaagcgcga cttcgtcgct gaggtgaaat ttcacgatac cttcgagggg atcctgaata
     5161 aattgtccga caagtatttt attatcagcg gcaacagtcc ggatttcctt ttcgaaatga
     5221 tatcctatcg agcctacaaa acctaccggt aaatcgcagc attcaggaat catgcgaaca
     5281 atgttgtcga agaaatcctg aaaagcatct ttcagcatta ctgccaactg tgcttgttca
     5341 cgatatttat gtataaacgg cacgaacgaa gccagataca cacttggagt agagcccctg
     5401 tacaacgaat tcattacgtt attgtaatcc atatcaagct cgttgcgaat tgtgcgcata
     5461 aagtcgtgat cgaatctgct tttgtagtaa gtatccagaa atttcttacc cagatacgct
     5521 ccgctgcctt catcaccaag caaatatcca agcgacataa ctttcctgaa tatattatca
     5581 ccattgtagg cgcaggcgtt ggcacccgtt cctaaaatta ttgcaattcc cctctccttt
     5641 tgccatactg cgcgcgcagc cccaagaata tcggtgtaaa cgtccacaat agcaacagta
     5701 aaagtgtttt ccagcgaatc gaccatcatc tgacgcctct cgctgtttcc acaaccggct
     5761 ccgtaaaaaa acacatgctg cacttcgagg gggtcgacat cggcaggaat tacggaattc
     5821 actgcagccg acacatcgcc ggttgtaacg aaataagggt tcaaccccat ggtttttttg
     5881 tgggcaacca catcaccatt gcctctcaca agcatccatt ctgttttggt agaaccactg
     5941 tcggcaaata aaaacatata attcggttta atgcttaaaa ttataagttt ctgcgtaatt
     6001 ttacaattaa aattttggaa tatttagtgc atcattgtta atttgattat ttaaaccaga
     6061 aaaagcccat tatgaaagcc atgattttag cagcaggact gggaacgcga ctcaagccac
     6121 ttaccgataa caaacccaaa gcgcttgtcg aaatcaatgg tgtgcctatg ctgggcctgc
     6181 ttatcgaaaa gctgaaaaga tatggttgcg ataatattgt ggtaaacata catcactttg
     6241 gacaacaaat tatcgattat ctttcccaaa acaacaattt taatatcagc attgctgtct
     6301 ccgacgaaag attcatgctt ctcgacaccg gtggaggtat aaaacatgca agaaaactgt
     6361 tgcctgaatc ggaagcattt attgttcaca atgtcgatat ttactctgat atcgatattg
     6421 ctgcaatgta tcgctatcat ctgagcagtg gtaatttagt aactctggct gtaaaacagc
     6481 gcgaaagcag caattatctt tatttcgaca gcaacaattt gctttgtggt tggaaaagct
     6541 ataaaactgg cagagaaatt atttccagac ccattgagca atacaaggaa ttggctttta
     6601 gcggaataca cattattaat ccatccatat tcgatatgat tactgaagaa ggctgcttcg
     6661 ggattgtgcc ggtatatctg cggttggctg caacgcattc tattggtgct tatctgcatc
     6721 agggcgacac cataatcgac ctcggaaaaa ccgaatcgct gcttgaagtt gaaagagcta
     6781 tactgcaaaa aaacgatgat ttacagtaaa atctctgtaa atcatctaaa acctgttcag
     6841 tacgagttaa tttgccacca aattctgcta cttgccttcg agaaattcgt tcagctcctc
     6901 aattgttgat tcaaactgaa aagcatcgtt caccttgtaa tagttatttt tatcgtaggc
     6961 aaaatcgtag gcatactttg caaactcaag gcgtgtcgat tcataatcga atatctccat
     7021 cacctttttt atctgatcgc aagtcataca attcgatttg gcaatttgtt ttgcaagggt
     7081 cagttgacta tcgctaaagc ttttttcttc gatacttttg ctggcactta aaaaatcggc
     7141 atcgctcatc gcatatctgc acccgccatc attatattgc tgcgggggca aggggtcgta
     7201 accgtgttga tgttctccat gttgatttcc atggtgatca tgtggtcttt cggccggttg
     7261 atccggtgtt tcgtaatgct gctgatcggt ttcggtgatg gtaaccgatt cgttgtaggt
     7321 tgtcgaagta gtggtagtta ccgtggtagt tgaggtagca ggggcatcgg gcacattgat
     7381 attaacattc agttgtacgt tttctccgtc gggatcagtt acctgcatcg aaactccgtt
     7441 ggaatttaca ttcagtgttg caccaacggt ttgggtattt ccgcccgatg tagtggtttg
     7501 tgtgtttata tcggtggtag tcacctgagt taccgtctgg gttgtaggtg gagtagtttc
     7561 aggcgtttcg taaacaactt cttttacgct ggtttgagtt tcgctggcgg gcagtgctgt
     7621 atagggagct tcgctgataa gccgcaacac gtattctcct tttttattct tcacaatcga
     7681 caccgtcatt tctttgttgg gctccagaaa tgtaattgat ttattcaatt ctggaagact
     7741 tttattgttg aatgctattt tcacacgata cgaatcggaa ttcaggtctt tcgacttcac
     7801 attcacttca ggcatctggt tctgctgttc tccattcatg aagagcagga atttttctcc
     7861 gtcatcggaa aacacaacaa gccggttctg agcatttgta acataagcca ctgcaacaac
     7921 gagcagtaat aagagacatt ttttcataat tacatatttt agtttacttc atttttttat
     7981 cgaaaaacgt gccagcttca aaagctttag ttctttttga aaacagtttt cattttcacg
     8041 gtataggtat attgtataac gggctccaac acgtcagggt taatcacctt gtcgaaataa
     8101 ttatcgggaa gtttactata gtacaggttt atcttggagt tctcattgat aaggctggct
     8161 gtgctcgaca atgccttcag gttgtcggga gtatacgaaa taaatgtttc gtagcgctgc
     8221 agcggataat agcaatagtg gctttcttcg taagtacggt acatattggc tgcatttacg
     8281 ccaagggtat tgtattcctg tgttttttta ttcaggatat cgatgcaatc cttacgcatg
     8341 gtgtcgagaa tcgactgcat atcattaaca atataatcta ctttgatgat atcgtaaatt
     8401 tcggaagcag cagcgttttt aattatttta tcggcttgtt tgcggtcgtt gtagcgaatg
     8461 tgaatgttct ttttcagctc aaagcctttg ggaaccttta catatttttt gctgaacagt
     8521 gttttctctt cttcgtagct gaaaatcgga gcctgcgaaa taaaatcgac ataaacatct
     8581 ttctgctgaa taccctctgc tccgagcgaa gtaataaaat cgttaatgcg ctggtcgaga
     8641 agctgatggc aaacctcaag gctctctccc acctgactgg ttcccagcat aataacaaaa
     8701 taatcgggct gcacattcat catcacattg gcaatgacat agcaaacagt atcggaaccc
     8761 ttgctgtatg taggcgacga attataatta gaataattgg cattataata attttgttga
     8821 ttcatttcgt agtcgtaatc cttgtattct tttttgaaat tcgggttatt gctttggctg
     8881 aagtaataat ttcccgagcc ctgcgaaaac accggcgttg ccatgcaaac aactgcagtg
     8941 aataataata gctttttcat aatgatggtt tttggtttat gaggtaaaaa taataaaaac
     9001 gctcgatgta tcaaaacaga taattgttag caaaatataa ccaacccgta tttcaatctt
     9061 tggtaaaatc gctgagtatc tggttcagtt catcttcggt gctgcgcagt tttgctttgc
     9121 atgtatttat aagatatgta gccctgcgta cattgtcggc aagctcgtca acatcaattt
     9181 cctgctgttc gattttttcg acaatttgtt ccaattcggc aagcgcctgc tgatagctga
     9241 ttttttcttt tcccatgtta atggttttat ccttttagtt taaccttgta ttttttcgac
     9301 agcagataat ttttaatttt ctcgcggaat tcgccttgta tatatatttc gccgtcttta
     9361 gccgacccgc ctgttgcaca atactgcttt aatgattttg ccaattccag caacgcatct
     9421 tccggaccgg agaaacctgt aatcagcgtc actgttttac cattgcgttg ctttcggtcg
     9481 atagctatgt aaagctgttg tttttcgggt ggcagcaact ccgattcggg agcttcgcca
     9541 tgactgaacg taaactccgg attggtggaa tacactaccc cttgccgcga tttccagttg
     9601 ttgtccatac ttgttgcgaa gatagtgttt tatttgttct ctgcgtattg ttatgaatca
     9661 aaaagctctt accaaccatt gaaatatgac aaccatcgaa aaatgaatag aaaacctttg
     9721 gtaaagactt ggttacacag agaataactg ttcaaacctg ctgatatcgc ccggaaaagt
     9781 gattttgaaa ttctcagggt tgcctttggt tacagccaaa tcggcattaa aagcgtatct
     9841 gaggtaaatg ctcgcatcgt cggtgggttc aatattgctt tgactgaaaa actcgcgata
     9901 ggcaaataaa atcttatcga gccggaaacc ctgaggtgtt tgcacgagtt ttatttcgct
     9961 gcggttcagt ggcttgggtt ggaatccttc tttcagttgc attacagtat cggcaaccgc
    10021 aataagcgga caggcagctt cgtactcact aagcgcttca acaacaccat tgatggttgt
    10081 ttcatccaca ttcggacgca ctgcatcgtg aataagcaca ttgccatcag gagccattgc
    10141 ctccgctttc agcaatccct ggtaaacaga gtgataacgc tgtgctccac cgcatactat
    10201 ttcaacgtgt ttaatttttc taaaatcgtt cattaactcc tctaccgtat caatataatc
    10261 ggtatgagcc accactataa taccagaaat aagcgggtgg tgaatgaatt ttgccacaca
    10321 atgttccagt atggtttttc cggcaacact gatgtattgt ttcggtatat cggaatgcat
    10381 cctgacaccc gatccacccg atactatgat tgcaatatta gttttcatgg ataacaagtt
    10441 gttctgcaaa tgtaagaatt gtttcgtaaa taggtttcaa cccaatccga gtcactcgta
    10501 gaggacttgg atgcaaccaa aggctaaaaa aaagtccatc aaccttatcg gcaaatggac
    10561 ttcctgttat aattaagcta tgttatgctt taatcagtgc ttcataatcg gctggggtca
    10621 gcaaatcttt caactcggca gggtccgaaa tttttatcct tatcagccat ccatcgccaa
    10681 agctatcgga atttactaat gtggaatcgt ttttcaccag ctcgtttgct tcgagcactt
    10741 cgcctgccac aggcatgtaa gaatcggaag cagttttcac agcttcgatc gaaccaaaca
    10801 cttcgccttt tgccagctct tcgccttcgg tttcgatttc gacaaaagca atttcgccca
    10861 gttgctcagc agcaaattcg gtgataccta taacagcttc atcaccttca acacgaatcc
    10921 attcatgggt ttttgtgtac agcagatttt ttggaatatt cataacaaca aatcgtttaa
    10981 taaattaata tgtaatttgt ttcaaagtaa ctaataaaat tactaagatg caagagtaaa
    11041 acgcaaactt acaccaaaat ttgtatttga attcggataa gacaagctta tcctcgggcg
    11101 gttaattacc tgatcgtaga atattcgcag attaaagcgg tcgctgagca cataatcagc
    11161 agaggtttta atggtaaata cccgctgccc tgcggtaggc tgatcgactt cttcgttcag
    11221 tttccggatt atggtaaggc tgttgcgata tgagaagtca accctcaggt tcaaatcgct
    11281 cttgaaatta cgctgcttcc ctcctgtttt aatggtaatt tcaacgtctt taaatcgata
    11341 gcccgaacca atcacaaatt catcggtcag gttctcggtg agctgattgt tggtaaagct
    11401 c
//
LOCUS       DAOOQP010000094        11377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7108_length_11377_cov_5.770447, whole genome shotgun sequence.
ACCESSION   DAOOQP010000094 DAOOQP010000000
VERSION     DAOOQP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7108_length_11377_cov_5.770447"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..745)
                     /locus_tag="PLK75_09570"
     CDS             complement(353..745)
                     /locus_tag="PLK75_09570"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOY39535.1"
                     /translation="MAKIGELEQDQVNIIGNGTVIKGDVVSTGDMRIDGLLQGNLQTK
                     GRLIIGATGKLIGEIDCKNSEISGTIEGKIVVGELLTLKSTAKIMGDIITSKLSIEPG
                     AIFTGTCNMGGNQRKPEVTPVTENASAK"
     gene            959..2221
                     /gene="metK"
                     /locus_tag="PLK75_09575"
     CDS             959..2221
                     /gene="metK"
                     /locus_tag="PLK75_09575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOY39536.1"
                     /translation="MGYLFTSESVSEGHPDKLADQISDAILDEFLAFDPQSKVACETL
                     VTTGQAVVAGEVKSKVYVDIQETTRRVINEVGYTKGEYRFDGNSCGVLVAIHEQSPDI
                     NQGVEEGKGLHAEQGAGDQGMMFGYACRETDDFMPLSLDLAHRLLQELAVIRREGVQM
                     TYLRPDSKSQVTIEYSDDNKPVRIHTIVVSTQHDDFDENEMKMLEIIKNDVKTILIPR
                     VKSQLSPSMASLFTNDFILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGA
                     FSGKDPSKVDRSAAYAARHIAKNMVAAGVADEMLVQVAYAIGVAQPVGIYVNTYATSK
                     VGMPDSVIAEKIAHIFDMRPAAIEQRLKLRNPIYRETASYGHFGRTPRKVTKQFCSPY
                     IEDKTVDVELFTWEKLDYIDKVKAVFNC"
     gene            2354..3481
                     /locus_tag="PLK75_09580"
     CDS             2354..3481
                     /locus_tag="PLK75_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOY39537.1"
                     /translation="MSKIYKYLIGSLGVILMILFVFLFPDITAWILISVVIAMIGSPL
                     VEFLSKIRFRKLIFPRWLAAIITLGAFYSIIFLFFWFFIPLVAGQINEFRKLDVTLIE
                     QGFDEPIRVVDSVINDYHLSADENFSVRQIATEKIQSIVNFSSVSNFLSNVTSTISTS
                     LLGLFAISFISFFFLKEKYLFDRGVIAMMPEKVEEKTRNVLASIRKLISRYFIGLILE
                     IICVMSLITGGLTLLGLKFELAILIGMFAGVFNIIPFLGPWIAAALGILLTITGNIGL
                     DFNSEILPLIYKLIAVFVVVRLIDDFILQPLIYSKSVKAHPLEIFLVIIVAASIGGVI
                     GMMLAIPVYTVLRVIAKEFFNHFKFVQEITKNMSIPEDKKP"
     gene            3487..4743
                     /locus_tag="PLK75_09585"
     CDS             3487..4743
                     /locus_tag="PLK75_09585"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOY39538.1"
                     /translation="MRIFKFGGASVKNAEAVRNLAKIVAAKNENIIVVVSAMDKITNA
                     LEQVHKHHKYASSEIAESFAYIRSFHDGIAGQLFEDSAHPVFSRLNSLYSEIEHIISQ
                     PPSENSAFDYDRLVSYGELISTCIVAEFLIFNGVDAQYFDIRKILRTDNTYREAKIQW
                     DVSAKLISKYLPQHGHKVIITQGFIGCTSEGLTTTLGREGSDFSAAILAYLLNAEDVT
                     IWKDVPGIMNADPAWYKESEKIDKLSYEEAIELAFYGAKVIHPRTVKPLQNKKIPLHV
                     KSFLNPNNSGTTIEEIRGNLVLPPIYILKNEQLLLSISPKDFSFIGEDHISHIFALFT
                     QFRTHVNLIQNSAISFSACADMNSDTYKELISELQINFKVKYNDNLLLITIRHYNEEA
                     IEKMISDKSVLLEQKSRSTVRFVVKQ"
     gene            4797..6068
                     /gene="purD"
                     /locus_tag="PLK75_09590"
     CDS             4797..6068
                     /gene="purD"
                     /locus_tag="PLK75_09590"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOY39539.1"
                     /translation="MNVLLLGSGGREHAIAWKLAQSRHCKKLFIAPGNAGTLSCGANV
                     ALDPMNFDRVADFVIENSIGLVIPGPEAPLVAGIYDYFRGRHDLSHVGIIGPGIEGAR
                     LEGSKMFAKEFMVRNNIPTAAYFTVTSETFHQGCRFLEELRPPYVLKADGLAAGKGVI
                     ILSDLQQAKETLNEMLNGMFGLASKSVVIEEYLDGIELSCFFVFDGTDYRVLPEAKDY
                     KRAGDNNSGLNTGGMGSVSPVPFANSEFKNKVEEKIIKPTVQGLINENIDYRGFVFVG
                     LMNVRGEPFVIEYNVRLGDPETESVLPRVKNDFLEMLMAVSSGTLKPITIDTDERCVV
                     SIMLVSQGYPGKYEKGKEIKCGSTTNNELIFHAGTKVLDGKIFSDGGRVISAGAFGNS
                     LAEAIKNAYAVSSGIGFENKYFRSDIGFDLL"
     gene            6065..7054
                     /locus_tag="PLK75_09595"
     CDS             6065..7054
                     /locus_tag="PLK75_09595"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOY39540.1"
                     /translation="MKTIFKRNHTFNHIFIGFLAVIIGLARYFVLVNSEIDVIQYHNI
                     VYSFNIVSNLFFIETIICISLLIVLSAYLNAFNSKHSIVEGAFQLPGMIFIALTGVFI
                     MDPDIVPVLTASCLIMVSLDRLFPVYRKPNALSNCFDAGFWPGLALFIYPDSLILFPL
                     IIIVLLYIKPFQLREVILMLLGLALPLIIMLSVFYLTDEWELLRENYFGNRLLQFTMA
                     KYNTANIVFASGVLILGVIALMYYLLAANFKKIVVRRYLAITAFFLIISGVESMTPVT
                     GFAFFLFYLIPLSYMLSNMVVNSKSRFLNAIIPLLIIFILGSYHYLYFSGFII"
     gene            7103..8284
                     /locus_tag="PLK75_09600"
     CDS             7103..8284
                     /locus_tag="PLK75_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY39541.1"
                     /translation="MNIYKKKKTWKLILFVAAVAIGGVSLLYTNLLMEKLSVEERKKV
                     ELWAEATKNLANGDPDQDVSFLFEVLKNNNTIPVILTDENDVIIAFRNIDSLKMADTV
                     YAKRVLISMKNHHEPIVIELFEGHYNKIYYKDSTLLTQLQYYPFVQLGIIFLFIIVSY
                     LAFSASRKAEQNHVWLGMSKETAHQLGTPISSLMGWVEIMKEQEQDREMISEMSKDIE
                     RLEKITARFSKIGSDPVLKLMNLNDVIHSAVTYIKTRASEKIRISVVHSNDEPIIVPL
                     NQELFEWVIENLCKNAIDAMDGKGEIQILISDTAKLVTIDVKDNGKGIPKSKYKTIFQ
                     PGYSTKTRGWGLGLSLSKRIIVQYHKGKIFLKSSEINQGSTFRIILSKGVGIEVRSNK
                     K"
     gene            8332..8865
                     /locus_tag="PLK75_09605"
     CDS             8332..8865
                     /locus_tag="PLK75_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOY39542.1"
                     /translation="MHKCSSYIVSIRLGVENSQTYCFDINERFFSLFEEPVIKAGELQ
                     ARLILTQEATSYLMQFFIEGFVQVECDRCLELCKQPVQVDTEIIANIGQETVFDTNTD
                     NISIASTDSELDVSGLLYELIILSLPYQRIHNSEYGENQVCRPDMIEILNRYIIEEEK
                     HIDPRWEKLQEINLKKQ"
     gene            8867..9052
                     /gene="rpmF"
                     /locus_tag="PLK75_09610"
     CDS             8867..9052
                     /gene="rpmF"
                     /locus_tag="PLK75_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOY39543.1"
                     /translation="MAHPKRRHSKTRSRSRRTHYKAVAPQLSTCSNCNASVPYHHVCP
                     ECGFYRGKLVIEKEVKV"
     gene            9072..10037
                     /gene="plsX"
                     /locus_tag="PLK75_09615"
     CDS             9072..10037
                     /gene="plsX"
                     /locus_tag="PLK75_09615"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOY39544.1"
                     /translation="MRIGLDVSGGDFAPQTTIDGAIMCLEDLVPDDIIVLLGNRKSII
                     AELHKRGVDAENFEIIDASQSIEMHEHPTKAFESKPQSSIVMGFRMLVESSLDGFASA
                     GNTGAMLVGAMQMVKPVNGIIRPCIASVYPNIQGGFNLLLDVGFNPDAKPEMLFQFGQ
                     IGSVFARNVYSCTKPKVGLLNIGSESGKGNLLSKSAFNLLSANDKINFIGNVEGNQLF
                     DSNVVDVIVTDGFTGNIVLKQAEGFYSLLMKQNLMNEYFKKYNFEFYGGTPVLGINKN
                     VVIGHGRSSAFAVKNMILLTAKIAKVNLSKKINEFMNYEKNQSNH"
     gene            10012..11019
                     /locus_tag="PLK75_09620"
     CDS             10012..11019
                     /locus_tag="PLK75_09620"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOY39545.1"
                     /translation="MKKIRATIKGVGGYVPEYVLTNEEISQMVDTSDEWIMTRIGIKE
                     RRILKEEGKATSDMAMLAVNELLMKTNTRPEEIDMLICGVVTPDMQFPATANIICDKT
                     GILNAFGYDVNAGCSSFLFSLVTATKFIESGTHKKVIVVGADKMSSITDYSDRKTSPI
                     FGDGAGAVLLEPSDDESGIYDSLMHVDGSGRHHLYQLAGGSLNPPSFETIMRRQHFIY
                     QEGQPVFKAAVSNMADISLEIMQRNNLNGETLNWFVPHQANMRIIDATARRMGIEDKN
                     KIMVNIEKYGNTTSATIPLLLWDYEKQMKKGDSVVISTFGAGFTWGACYLKWAYDGIT
                     I"
     gene            complement(11085..>11377)
                     /locus_tag="PLK75_09625"
     CDS             complement(11085..>11377)
                     /locus_tag="PLK75_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY39546.1"
                     /translation="ITIIDVQEDCIRIPNAFTPNADGVNDTWIIDNIDMYPRAYIEVF
                     NRWGQCIFEAKGSEDPWDGTYNGKIVPTGPYIYVVNLFNGDEAYTGTVTIVR"
BASE COUNT         3479 a         1983 c         2399 g         3516 t
ORIGIN      
        1 agagtaagga aaaataaaaa aatgtgatat gtgtaaaacc ccttggggct ggtgtatcca
       61 agtaaaaaat agcttataag ccagtgggtg caaaaaaaca gaataccaaa aatcaaataa
      121 atgattccat cggagaggta ttttttcatt tgtttattca cgtggtattt gtttaattaa
      181 caagaaaatc gcagcaaata caccaaacaa ggaaagcact acagtaaaga caggaaattt
      241 aatgtttagt ttatcatcaa gccaaacacc aaggtaaaca catccaaaaa taattgcaat
      301 catttcgacg acgattgccg aaaatctggc atacttctga aatggattat cgttacttgg
      361 ctgaggcatt ttcagttaca ggagtaactt cgggtttacg ttgattgcca cccatattgc
      421 atgtgcctgt aaaaatcgca ccgggttcaa tagacaattt cgaagtgatg atatcgccca
      481 ttatctttgc cgttgatttt aaagtaagca attcaccaac aacaattttg ccctcaatgg
      541 tacccgaaat ttcggagttt ttacaatcga tttcaccaat aagtttaccg gtagcgccaa
      601 taataagcct tcctttggtt tgaagatttc cctgcaacaa gccatcaata cgcatatcgc
      661 cggtcgaaac cacgtcgccc ttaattacag taccattgcc aataatgtta acctgatcct
      721 gctcgagttc gccaattttt gccatgtttg tgtgcttttg tttgttaccg actacaaata
      781 tattaatatt catttattaa taaataaaag tttttggatt ttaaacaatc ttgctatttt
      841 tgtgttttca tagtggaaaa atttgggtga ttgcaaccac agaaaaagaa tgctaaaaca
      901 ccgttctgca ctacaatccc caaaattatt tgaatgttta attttaattt attaattaat
      961 gggttactta tttacatccg agtcagtgtc tgaagggcat ccggataaat tggcagatca
     1021 gatttctgat gcaattcttg atgaatttct tgctttcgac cctcagtcga aagttgcctg
     1081 tgaaacattg gttactaccg gacaagctgt tgtggctggt gaggttaaat cgaaggttta
     1141 tgttgatatt caggaaacta cccgcagggt gataaatgaa gtcggttaca caaaaggaga
     1201 atatcgtttc gacggtaatt cgtgtggggt tttagtggct attcacgaac aatcgcctga
     1261 tatcaatcag ggtgttgaag aaggcaaagg ccttcatgct gagcaaggag caggagatca
     1321 ggggatgatg tttggttatg catgtcgtga aaccgacgac tttatgccat tgtcgctcga
     1381 cctggcgcat cgtttgctgc aggaactggc tgtaataagg cgtgaagggg ttcaaatgac
     1441 ctatttgcgg cctgattcaa aatcgcaggt taccatagaa tacagcgatg ataataagcc
     1501 tgtacgaata cacacaatag tggtttcaac gcaacatgac gacttcgatg aaaacgaaat
     1561 gaaaatgttg gagattatta aaaatgatgt taaaacaata ctgataccta gggtaaagtc
     1621 acaattatcg ccaagtatgg cctcattatt tacaaacgat tttatccttc atgtgaatcc
     1681 tacgggaaaa ttcgtaattg gtggtcctca tggagacaca gggctgaccg ggcgcaaaat
     1741 catagttgat acatatggag gcaaaggtgc tcatggcgga ggcgcattct caggtaaaga
     1801 tccatcaaag gttgacaggt cggccgctta tgcagccaga cacattgcta aaaacatggt
     1861 tgcagcaggc gttgccgacg agatgcttgt tcaggtggct tatgccattg gcgttgctca
     1921 acctgttggt atttatgtga atacttatgc cacatcaaaa gttggtatgc ctgatagtgt
     1981 gatcgctgag aaaattgcac acattttcga tatgcgaccc gctgcgattg agcaacgact
     2041 gaaactacgt aacccgatat atcgcgaaac agcgtcatac ggacatttcg gaagaactcc
     2101 ccgaaaagtt acaaaacagt tctgctcacc ctatattgag gataaaaccg ttgatgttga
     2161 actttttaca tgggagaaac tcgattatat cgataaagtt aaggctgtgt ttaattgtta
     2221 atattgccaa aacacaatgc gaacagagct gtatcaattg atacggctct gttattttgt
     2281 ggtgatgatt atttggaaat tgtaaaacat ttcaatcctt tttcgcatct ttgtagttca
     2341 tacaaaaaaa actatgtcga aaatatacaa atatcttatc gggtcgttgg gggtaattct
     2401 gatgattctt tttgtatttt tatttcctga tattactgca tggattctga tatctgttgt
     2461 aattgccatg attggatctc ccttagtgga gtttctcagc aaaatcagat tcagaaaact
     2521 gattttcccg aggtggcttg ctgcaataat tacattggga gctttttact ctattatttt
     2581 tctctttttc tggtttttta ttccactcgt tgccgggcag attaacgaat tccgaaagct
     2641 ggatgttact ttgattgagc agggctttga tgagccaata cgcgttgtgg attcggtaat
     2701 taacgattat catttgtcgg ctgacgagaa tttttctgta agacagatag caaccgaaaa
     2761 aattcaaagt atcgtgaatt tttcgagcgt gtccaatttt ctgagcaatg ttacaagcac
     2821 tattagtact tcgctgctgg gtttgtttgc catttctttt atttcgttct ttttcctgaa
     2881 agagaaatat cttttcgaca gaggtgtaat cgcaatgatg ccggaaaaag tggaggaaaa
     2941 aaccagaaat gtgctggctt ctataagaaa actgatttcc agatatttta ttggtcttat
     3001 ccttgaaata atttgtgtga tgagcttgat taccggtgga cttacgcttc tggggttgaa
     3061 atttgagctt gctattctta ttggaatgtt tgcaggcgtt tttaacataa taccattcct
     3121 gggcccctgg attgctgctg cattaggaat attgctgact attacgggaa atattggttt
     3181 agattttaat tcagaaatac ttcctttaat ttataaactt attgcagtat ttgttgttgt
     3241 gcgactcatt gatgatttta tacttcaacc attaatctat tcaaaaagcg taaaagccca
     3301 tccccttgag atatttttgg ttattattgt cgctgcctcc attggaggtg tcattggaat
     3361 gatgctagcc atccccgtat acacagtact acgggttatt gcaaaagagt tttttaatca
     3421 ttttaaattt gtgcaggaga taactaagaa tatgagtatt cctgaagata aaaagccctg
     3481 aaagagatga gaatatttaa gtttggagga gcttctgtga agaatgcaga agctgtcaga
     3541 aacctagcaa agatagttgc agcgaaaaat gaaaacatta ttgttgttgt ttctgccatg
     3601 gacaaaatta ccaatgcatt agagcaggta cacaagcatc ataaatatgc aagcagtgaa
     3661 attgcagaga gtttcgcata tattagaagt tttcatgatg gtattgcggg gcaattattc
     3721 gaagattcag cccatccggt tttttccaga ttgaattcgt tatattccga aattgaacat
     3781 attattagtc aacccccttc cgaaaactca gcattcgatt acgacaggtt ggtgagttat
     3841 ggcgagttga tttcaacgtg tatcgtagct gagttcctta ttttcaatgg ggttgatgcg
     3901 caatattttg atatacgcaa gatcttaaga acagacaata cgtaccgcga agctaagata
     3961 caatgggatg tttcagcaaa actgatttca aaatacctgc ctcaacacgg acataaagtt
     4021 ataataaccc agggttttat tggatgcacg tcagaagggc ttacaacaac acttggtaga
     4081 gaaggatccg atttttcagc agccatcctt gcttacctgc taaatgcaga agatgttact
     4141 atctggaaag atgttccggg aataatgaat gctgaccctg catggtacaa agagtcggaa
     4201 aaaattgaca aactgtcgta tgaagaagca attgaacttg ctttttatgg cgcaaaagtg
     4261 atacatcccc gaacggttaa accgttgcaa aacaagaaaa taccgcttca tgtaaaatct
     4321 tttctcaatc caaacaactc aggtacaact atcgaagaaa tcagggggaa cttggttctt
     4381 cctccaatat acattcttaa aaatgaacaa ttattgcttt cgatttctcc caaagatttc
     4441 tcttttatcg gtgaagacca catcagtcat atatttgctc tctttaccca attccggacc
     4501 cacgtgaatt tgatacagaa ctcagctatt agtttttcgg catgtgccga tatgaattcc
     4561 gatacgtata aagagctgat ttcggagctg caaattaact ttaaggtgaa gtacaacgat
     4621 aatttgttac tgataaccat caggcattat aatgaagaag caattgaaaa gatgattagt
     4681 gataagagtg ttttgctaga acaaaaaagc cggtcaaccg tgcggtttgt tgtgaagcaa
     4741 taagctcttt caattgacct tctgcaaaaa taagttgcat ttaaaaagtt ctacacatga
     4801 atgttttgtt attaggatca ggtggcaggg agcatgcaat tgcgtggaaa ttggcacaga
     4861 gtcggcattg caaaaaactg tttattgcac caggtaatgc cggaacctta agttgtggtg
     4921 ccaatgtagc cctcgatcca atgaattttg atagggttgc tgactttgtt attgaaaata
     4981 gcattggctt ggttatcccc gggcctgaag caccattggt tgctggaata tacgattatt
     5041 tcaggggccg gcacgatctg agccatgttg gaattattgg tcccggtatt gaaggtgcca
     5101 ggttggaagg gagtaaaatg tttgccaaag agtttatggt tagaaataat attcccacag
     5161 cggcttactt tacggttact tccgaaacat tccatcaggg atgccggttc cttgaagaac
     5221 ttcggcctcc ttacgttctt aaggccgatg gtcttgcagc aggaaaaggt gtgataattc
     5281 tttctgattt gcaacaggca aaagaaacct tgaatgaaat gctgaatggg atgtttggat
     5341 tggcaagcaa gtcggtcgta atcgaagaat atctcgatgg tatcgagttg tcttgttttt
     5401 ttgttttcga tggtaccgat tatagggttt tacccgaggc aaaagattac aaaagagcag
     5461 gcgataacaa ctctggtctt aacaccgggg gcatgggttc tgtttctcct gtgccatttg
     5521 ctaactcgga gtttaaaaat aaagttgaag agaaaattat taaaccaact gtgcaaggac
     5581 ttataaatga aaacatcgat tatagaggat ttgtttttgt gggactgatg aatgtacgtg
     5641 gtgagccatt tgttattgaa tataacgtga gattgggaga tccagaaaca gagtcggtgt
     5701 taccccgtgt taaaaacgat tttcttgaaa tgctgatggc agtaagttcc ggaaccctga
     5761 aacccattac tattgataca gatgagcgat gcgtagtaag tataatgctt gtttcgcaag
     5821 gctatcccgg gaaatatgaa aagggaaaag aaattaagtg cggtagtaca acaaataacg
     5881 agttgatatt tcatgcaggg acgaaggtat tggatggtaa aatattttcc gatgggggca
     5941 gagtaatttc tgcaggtgca tttggcaatt cgttggctga agccattaaa aatgcctacg
     6001 ctgtttcatc gggaattggt tttgaaaata aatacttcag gtctgatatt ggtttcgatc
     6061 tgttatgaaa actattttta aacgaaatca tacttttaac catattttta ttggctttct
     6121 tgccgttatt atcggactgg cgcggtattt tgtcctggtg aattcggaaa tagacgtaat
     6181 acagtaccat aatattgttt acagcttcaa cattgttagc aatctgtttt tcattgaaac
     6241 tataatatgc atctcgttac taatagtatt gtcggcatac ctgaatgcct tcaacagcaa
     6301 acactctatt gttgaaggag catttcagct tcccggaatg atatttatcg cactgacagg
     6361 tgtttttatt atggacccgg atattgttcc ggttcttacc gcatcctgtt taataatggt
     6421 atcgctcgac agacttttcc cggtgtaccg aaaaccaaat gcgctttcga attgcttcga
     6481 tgctggtttt tggcctgggt tggcactgtt tatatatccg gattccctca ttttattccc
     6541 gttgattatt attgtattgc tctatattaa gcccttccaa ttgagagagg ttattttaat
     6601 gcttcttggc cttgcattac ccttaataat aatgttatcg gtgttttatt taactgatga
     6661 atgggaactg ctaagggaaa actatttcgg taaccggtta ttgcaattta ccatggcgaa
     6721 atacaacaca gccaacatcg tttttgcttc aggtgtatta atactgggtg taattgcact
     6781 gatgtattat ttattggcgg ccaatttcaa aaaaatagtg gttaggcgtt atctggcaat
     6841 tactgcattt tttctgatta tttcaggagt tgaatctatg acaccggtaa ccgggtttgc
     6901 ttttttcttg ttttacctga ttcctttgag ttatatgctt tcaaatatgg tggttaattc
     6961 taagtcacgc tttctgaacg ccataatacc tttacttatt atatttattt tagggtcata
     7021 ccattatctc tattttagcg gatttattat ttgaaaatac tatatgagtt atgaaaacgt
     7081 ttcttaatca aatctgaata cggtgaatat ttacaagaaa aaaaagacct ggaaacttat
     7141 acttttcgtt gctgctgtgg caataggagg ggtgtcgttg ttgtatacaa atcttttaat
     7201 ggaaaaactt tcggttgagg aacgaaagaa ggtggaatta tgggcagaag caactaaaaa
     7261 cctggcaaac ggcgacccgg atcaagatgt gagcttttta tttgaagttc taaagaataa
     7321 taacacaatc ccggtaattc taactgatga aaacgatgtc ataatcgctt tccgaaatat
     7381 cgattcattg aaaatggccg atactgtata tgccaaacgg gttctaattt ctatgaaaaa
     7441 tcaccatgag cctatcgtaa ttgaattgtt tgaaggtcac tataataaaa tttattacaa
     7501 agattctaca ttactgactc aattacaata ttaccctttt gtgcaactcg gaataatttt
     7561 tctgtttatc atcgtttcgt atcttgcatt cagcgcatca agaaaagcag agcagaatca
     7621 tgtgtggctt ggaatgtcta aagaaactgc acatcagttg ggtacgccca tttcgtcgtt
     7681 gatgggttgg gtcgaaatta tgaaggagca ggaacaggat agagaaatga tatcggagat
     7741 gagtaaggat atcgaaagac ttgaaaagat tactgcccga ttttctaaaa tagggtctga
     7801 cccggtattg aagttaatga atctgaatga cgtaatacac agcgctgtaa cctatattaa
     7861 aacaagagct tcggaaaaaa tccgaatttc ggttgttcac agcaatgatg aacctatcat
     7921 tgttcccctg aaccaggaat tgtttgaatg ggttattgaa aatttgtgta aaaatgcaat
     7981 cgacgccatg gatggcaaag gtgaaataca aatattaatt tcagatactg caaaattggt
     8041 aacaatcgat gtaaaagaca atggcaaagg cataccgaag tcgaaatata aaacaatttt
     8101 tcaaccgggt tattccacaa aaacaagggg ttgggggttg ggcctttcat tatcgaaaag
     8161 aataatagtt caataccata aaggcaaaat attcctgaaa agctccgaaa taaaccaggg
     8221 ctccaccttt agaataattc tcagtaaagg tgtcggcatt gaagtaaggt caaataaaaa
     8281 ataattggca gaatagaaat tgttttatta attttgccga aattttttac tgtgcataaa
     8341 tgctcatcat atattgtttc tataaggctg ggagttgaaa attcacagac ttactgcttt
     8401 gatattaatg agcgtttttt ctctcttttt gaggagccgg ttattaaagc aggggagttg
     8461 caagcccggc ttatccttac acaggaggct acatcgtatc tgatgcaatt ttttattgaa
     8521 ggattcgttc aggtggagtg cgatcgctgt cttgaattat gcaagcaacc tgtgcaagtt
     8581 gacactgaga taattgcaaa tattggtcag gaaacggttt ttgatacaaa caccgacaat
     8641 atcagtatag cctctaccga tagtgaactt gatgtatcgg gtcttttgta tgaattaatt
     8701 atattatcgc ttccttatca acggattcat aattctgaat acggcgagaa tcaggtttgt
     8761 aggcctgata tgattgaaat tttaaacaga tatattattg aagaagagaa gcatattgat
     8821 ccccgctggg aaaaattaca agaaataaac cttaaaaaac aataaaatgg cacatcctaa
     8881 acgaagacat tcaaaaacaa gaagcagaag tcgtcgtact cattacaaag cagttgctcc
     8941 gcaattgtca acatgttcga attgcaatgc aagcgttcca tatcatcacg tttgccctga
     9001 gtgtggtttt tacagaggta aactggtaat cgaaaaagag gttaaggttt agttttctca
     9061 taaactacat tatgagaatt ggccttgatg tttcaggcgg tgattttgct cctcaaacaa
     9121 caatagatgg agcaattatg tgtttggagg atcttgtacc tgatgatatt atcgtattat
     9181 taggcaacag aaagtccatt attgctgaat tgcataaacg cggtgtggac gcggagaatt
     9241 ttgaaataat cgatgcttcg caatccatcg aaatgcatga gcatcctacg aaagcttttg
     9301 aatcgaaacc ccaaagcagc attgttatgg gattcagaat gcttgtagag tcatctcttg
     9361 atggatttgc aagtgcagga aacacaggtg ccatgctggt cggtgccatg caaatggtaa
     9421 aacccgtcaa cggaataata cgtccttgca ttgcctcagt atatcccaat atacagggag
     9481 gctttaacct tttactggat gttggattta atccggatgc caaacccgaa atgctctttc
     9541 aatttggaca aattggttct gtttttgccc gtaatgtata ttcatgcaca aagcctaaag
     9601 ttggtctttt aaatattggg tctgaatccg gaaagggaaa ccttctttcg aaatcagcct
     9661 ttaatttact atcagcaaac gacaaaatca attttatcgg aaatgtagaa gggaatcaat
     9721 tatttgattc caatgttgtc gatgttattg ttaccgatgg atttactggc aatattgttc
     9781 ttaagcaggc cgagggtttt tattctttac tgatgaagca aaaccttatg aatgaatact
     9841 ttaaaaaata taattttgaa ttttacggtg gaactccagt gctgggtatt aataaaaatg
     9901 ttgtaatcgg gcatggaaga agcagtgcat ttgctgttaa aaacatgatt cttcttacag
     9961 ccaaaattgc aaaagtgaat ttaagtaaga aaataaacga attcatgaac tatgaaaaaa
    10021 atcagagcaa ccattaaagg cgtcggggga tatgtacctg agtatgtgct tacaaatgaa
    10081 gaaatatcgc aaatggtaga tacttctgac gaatggataa tgaccagaat tggtattaag
    10141 gaaaggcgca ttttaaaaga agaagggaaa gctaccagcg atatggcaat gcttgctgtt
    10201 aatgaactgt tgatgaaaac caatacacgt cccgaagaga tagatatgct catttgcgga
    10261 gtggtaacac ctgatatgca attccctgct acagccaata ttatatgcga taaaacagga
    10321 attcttaatg cttttggcta cgatgtaaat gcgggttgct ccagcttttt attttctctt
    10381 gttactgcaa caaagttcat tgaaagcggt acacataaaa aagtaatcgt tgtgggtgct
    10441 gacaaaatgt cgtcaattac cgattactcc gatcgcaaaa caagtcccat ctttggagat
    10501 ggagccggag ctgttcttct tgaaccatct gatgatgaaa gtggaattta tgattccctg
    10561 atgcatgttg atggttcggg taggcaccat ttataccagc ttgctggagg ctccttgaac
    10621 ccacccagct ttgagacaat aatgcgcaga caacatttta tttatcagga aggtcaaccc
    10681 gtgtttaaag ctgcggtttc aaatatggct gatatttccc tcgaaattat gcaacgaaat
    10741 aacctcaatg gtgaaactct taactggttt gttccgcatc aggcaaacat gcgcatcatt
    10801 gatgccactg cccgaagaat ggggattgag gataaaaaca aaataatggt gaatatcgaa
    10861 aagtatggaa acactacttc tgcaactata ccattattat tgtgggatta tgaaaaacaa
    10921 atgaaaaagg gtgacagtgt agtaatttcg acttttggtg ccggctttac gtggggagct
    10981 tgctatctga agtgggctta tgatggaata acaatctaat tacttcgcta ttaataaaag
    11041 ggctgcccgg agaaaatcgg gcagcccttt ttatttagta gcccttatct tacaattgtt
    11101 actgtacctg tataagcttc atcgccgttg aaaagattaa cgacgtagat ataaggtccc
    11161 gtgggaacaa tttttccatt gtaagtacca tcccaggggt cttcgcttcc cttagcttcg
    11221 aagatgcact gtccccagcg gttgaaaacc tcgatgtatg ctctgggata catatcgatg
    11281 ttgtcgataa tccaggtgtc gttcactcca tcggcatttg gtgtgaaggc attgggtatt
    11341 ctgatgcaat cttcctgcac atcgattatt gtaattg
//
LOCUS       DAOOQP010000095        11308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7191_length_11308_cov_4.878255, whole genome shotgun sequence.
ACCESSION   DAOOQP010000095 DAOOQP010000000
VERSION     DAOOQP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7191_length_11308_cov_4.878255"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1037
                     /locus_tag="PLK75_09630"
     CDS             153..1037
                     /locus_tag="PLK75_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY39547.1"
                     /translation="MENNVIIINENCRLCKSCVEVCPNQIFALNENHVVVKPERTHLC
                     FACGHCMAVCAHNAVVVEGLNLERDFFDLPSATNSSEAFRCLLQTRRSVRVFKNTPVP
                     EEMLQMIAEAVSFSPPSFPPVKTELVIINGREKLEAALPQMVAMYEKMVKGLSNPIAK
                     TFIKKSMGHEKYKIVKNHLLPLLSVKLPYMLAGTEDSIFRGAPACMLFHADREADNAV
                     PDIYIAVGFAILAAHSLGLGATVVDLIAPAVERTPALRALFSIPEGNKVVAAVILGHP
                     KYRFKKGIKRQLKSVVYM"
     gene            1077..1337
                     /locus_tag="PLK75_09635"
     CDS             1077..1337
                     /locus_tag="PLK75_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39548.1"
                     /translation="MKQIIRLAYYNKSEWNYLLDIIDDRCSLPESWEAWFESHKNMKR
                     DIVAGGNKVREIKIVVDELIEYCELRQLKIDTKAINQFVKNY"
     gene            complement(1494..1892)
                     /gene="mscL"
                     /locus_tag="PLK75_09640"
     CDS             complement(1494..1892)
                     /gene="mscL"
                     /locus_tag="PLK75_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOY39549.1"
                     /translation="MGIVKEFKDFAMRGNVIDMAVGIIIGGAFGKIVSSLVNDVIMPP
                     IGYLLGGVNFTDLKVTISEATAEKAAVTLNYGNFVQVVIDFIIIAFVIFLMIKGMNSM
                     KKKKEEAPAAPPAPSKEQELLTEIRDLLKK"
     gene            complement(1914..2891)
                     /locus_tag="PLK75_09645"
     CDS             complement(1914..2891)
                     /locus_tag="PLK75_09645"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOY39550.1"
                     /translation="MKRKLLLILLVVLNFNLVIAQVTDGEKTLRNANTTTDDGWKTGG
                     VLSINLAQASFVNWAAGGQNSLGINGLFSAFATYHKGKSSWDNMLDVGYGILKQGKNS
                     SFIKTDDKIDFSSKYGRQASKSWYYAAILNFKTQMTNGYNYPNDSVAISKLMAPAYLI
                     GAIGMDFKPCADFTAFIAPFTSKTTFVLDEDFANAGAFGVTPAIYTDGLLTTPGKKVR
                     SEFGGYIRVVYTHSFFKEKNVTVLSKLDLFSNYLHNPQNIDVNWENIFSFKVNKFISA
                     TITTQLMYDDDIIIAVDRNDDGVINATDGPGGPRLQFKEILGIGFTYKF"
     gene            complement(3107..3760)
                     /locus_tag="PLK75_09650"
     CDS             complement(3107..3760)
                     /locus_tag="PLK75_09650"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOY39551.1"
                     /translation="MNIVFLIILLAIAFIGIITAEATDWQKLRYVTKPFIMPVMALLY
                     TFITHNFSPIIIAALGFSFGGDIFLLWPKNNKCFLFGLIAFLIAHGLYIYGILQWLIV
                     FHKIPAIAFTSVIIYFVIGICIFNLLKKGLGKLKIPVIVYMSFLLGMSFLSMLLFIFN
                     CSYTTGFVFTGSIFFIISDSILALHNFRKPKRFAGVYFMITYVMAQAFIVIGFSELF"
     gene            complement(3878..3950)
                     /locus_tag="PLK75_09655"
     tRNA            complement(3878..3950)
                     /locus_tag="PLK75_09655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3915..3917),aa:Pro,seq:cgg)
     gene            4118..6781
                     /locus_tag="PLK75_09660"
     CDS             4118..6781
                     /locus_tag="PLK75_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY39552.1"
                     /translation="MSRSNKKTTGKQRSNGTSAQEIGWSRKMESVLLANSDLTFVIDK
                     NGVFKELHYSDPSLLYVQPDNIIGMKVDQLLPGDVAEITMHKIGRVIETDKKEQYVYA
                     LGGHTFRSTITKIDDDTVIALINNIEEEIGLRENLNLTKQNYRKLIENMPLGIVICRM
                     IYDDNNKCIDFNVVSVNKEFIIITNETDIVGKQLSNVFSEIESQWFALCLKAANSGKA
                     VSHESYDPWMDKWISMTGYPTGQEEFVMVMTDLGKSVEQRYLIRKNEERYSFALKNSK
                     AFVWEWNRQMEMSYFSESLFSILGYVKTDLPRTFRSVLDMLHPDDVGTLKNVFAELHN
                     RVNDQFSVQLRFRKKNGEYVWLETQGKVAEFEANDLPYRIIGSCLDITSIKAKEIRLQ
                     ISETIFKSIFNNSPIGIGLFDLAGNLILANEAAIKIFKITENPEAKKLNIFTGNIIPA
                     EYLNQVKNLKPVDFSAWFRTEKLLLQGIEITEKKRVFLHCRFTPYNINLESNNVDRLL
                     LQVTDNTYEKQHERELLISKKYHENAMEIARLAFWSYFFDEKKLNISSEFMHIFELSN
                     SNDIENIEIMKRIISPDDWEFLVQFYNDVVSQSIEKEAEYEFIIVTQSGEKHVFSKVA
                     VIYEKGNKPIGIFGVLQDITERKKYEIELKKAKESAEESDRLKTAFLANMSHEIRTPL
                     NSIVGFSSLLANDMFDNAERTEFAKRIEFNSNQLLKLISDIIDIAKIESGSLTVIWEP
                     VLLLELLQNVYHANKINCPENVSFFVDFKGIEPDLQVQTDLVKFTQIITNLLSNAFKF
                     TEKGKIEFSATILNNNIRINICDTGIGIRQEELDMIFDRFYQANHFKTGTGLGLSICK
                     TLTEAMGGTIEVTSTLHEGSRFTVNLPLKIS"
     gene            6781..7548
                     /locus_tag="PLK75_09665"
     CDS             6781..7548
                     /locus_tag="PLK75_09665"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HOY39553.1"
                     /translation="MKKTIFAVILSIVIFSQYVAAQGAYNGVVVVCDIDDTYKITNTT
                     SKFSSVINALFSSRTFGGMNELLLGMNARGAQIYFLSNSPKVLKRRIIKLLKENQIEF
                     GGLFLRKTGEDGISHKITVLTSMIDTCSRQSFILIGDDSENDPLIYDSIKHLYPENIE
                     AIYIHHIKGKNLRTFYRFYSAVDIAYYESIVGRMNDVGTEKILDAVMASELHSVFPDY
                     IVFDEKLILSDVAGCFDRHCLSKNKTIEWLIDCWYKQ"
     gene            7605..9524
                     /locus_tag="PLK75_09670"
     CDS             7605..9524
                     /locus_tag="PLK75_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOY39554.1"
                     /translation="MKKLLVVIVCAILSLPVFAQEVDISSEKVLIDGKKFYLHTVKQG
                     QTLYGISKAYNVSTDDIIRINPESSNGVKTGQILKVPFNEVSNNEPVSGNPGKDKETH
                     HVSNPIPEGAITHKVEKKETIYSICKKYNLSVEALYKANPSIQEKGLRAGDVLVIPES
                     DLITDATKPEPEVQKPEPENPKDDSIGFVNHIIVKGETLSAISKKYGVTIAEILKYNP
                     GVSADKIKEGDKLQIPRKPNEQAAFPPEQRNHNGQGGDTLNAKDSRSIEYKIKNEINI
                     IMMLPFEVYVNTKNWYNQELGSKEYTVLPVTEMMLSYYGGCLMAFDSIKQTGLKVNMH
                     VFDTGSDTSSLGSLVNKDIFANSDLIIGPVYSKQINYIKDFIPETAVLLSPFSDYEKV
                     DIGDKSVLWANPGKIGRFEALAAFAAKNASNKYIIIYNNDGKSKEDAEELQKMMNESF
                     ALAGKKDSLNVSVLSSSEANGASLGKLLSHSGLNVIICDDKSEAQLSSLMAQLVQIKD
                     VDIQLIGEESWLNYRSIDVNYFQKLRFSYTTPYYIDYTNDKTLRFIIAYRQIFLSEPD
                     EFSFAAYDQMLMFSSLYMKHGGNLADAFNMKVPYCGMVSKNNFVNISGSRQYVQSFCN
                     LLMVDEDFQIKRIHP"
     gene            9686..>11308
                     /locus_tag="PLK75_09675"
     CDS             9686..>11308
                     /locus_tag="PLK75_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOY39555.1"
                     /translation="MMRKAGRLSAIMVFVMIFCIFLTQPRVQACNYSSTFAGTTITPT
                     ATWQTAGSVQAGARYQFTASQYMVYVFSYCDGGGSNSGDTQIEIFSAAGVSMAENDDF
                     CTLGSKLTWVAPASGTYYVVTFKYNCLADHTVMGTMAYKVMTQPNNQDCLGAIPLCFN
                     TYSTTISYSGVGNYTGEIPTTGGCPGNCLLSGERNDVWYTFTVQSSGTVSFLIDPVDN
                     DDDYDWAVYNLTNNECATIASNPSSVQVSCNYTATAGNTGPNGGSTNTCIGASGTPYN
                     AALNVTAGQTYVVNVSNFSSTQNGYSITFGGSAQILDNSDPFLTGIVYTPVCGQNNIT
                     VQFSENVTCLSISPSYFFVTGPGGTYTISDVVSSICAANGSYDDTYTLMLSDPLSDGG
                     TYTVGLTSTNGINDICYNQAVNNGNFTFSFTGLTASGSVTHNVTCYGGTDGAATVTSV
                     SGGTPPYDYTWSNGATTQSVANLPGGVAYVTVSDFYDCQEVLTFNITQPSQIIVEAGT
                     SQPVCPGNGITLGGSPTITNGVPPLTYGWSPTT"
BASE COUNT         3606 a         2049 c         2369 g         3284 t
ORIGIN      
        1 aacaggagat agccttgtgg catgcattgt gaacagaaag tacaaagttg taaagcttaa
       61 ttcggcttag gagacaagag cgattcgatt atagattgtt tttaaggact gatttgttga
      121 atataggtga gtaataataa taccttaaat atatggaaaa caatgtgatt attattaatg
      181 aaaattgtcg gttatgtaaa tcatgtgttg aggtttgtcc gaaccagatt tttgccctga
      241 atgaaaatca tgtagttgtt aaaccggagc gtactcatct atgctttgca tgcggacatt
      301 gtatggcagt ttgtgctcac aatgctgttg tggtagaagg tttaaacttg gagcgcgatt
      361 tttttgattt accgagtgct accaatagta gcgaagcgtt tcgatgctta ctgcaaacac
      421 ggaggtcggt acgggtattc aagaataccc cggttcctga agagatgttg caaatgattg
      481 cagaagcagt aagcttttcg cctcccagct tcccgccggt gaagacagag ctggtgatta
      541 tcaacggcag ggaaaaatta gaggcagcgc ttccgcaaat ggttgcgatg tacgaaaaaa
      601 tggtgaaagg gctttccaac cccattgcga aaacatttat aaaaaaaagt atgggtcatg
      661 agaagtataa gatagtcaaa aatcacctcc tcccattact gagtgtaaaa ctaccctata
      721 tgcttgccgg cacagaagat tcaatattca ggggggctcc tgcttgtatg ctttttcatg
      781 ctgacaggga ggctgacaat gcagttcccg atatttacat agctgttggc tttgccattc
      841 ttgcggcaca ttctcttggg ctgggagcaa ctgtagtcga tttgatagca cccgcagttg
      901 aacgcacccc tgcactgaga gcattgtttt cgatacccga aggcaacaag gttgttgcag
      961 cagttattct cgggcacccg aaataccgct ttaaaaaagg tataaaaaga caactgaaaa
     1021 gcgttgttta catgtagaaa aaatattaaa attgcaactc aaaccttaat tgccggatga
     1081 aacagataat acgccttgct tactataata agagcgaatg gaattatctg ctcgatatta
     1141 tcgacgacag atgcagtctg ccggaatcat gggaagcatg gtttgaaagt cataaaaata
     1201 tgaagaggga tattgttgcc gggggaaata aggttaggga gattaaaatt gttgtagatg
     1261 aattgattga atactgcgaa ttgcggcaac ttaaaatcga taccaaggca atcaatcagt
     1321 ttgtgaaaaa ctattaagct gtcccgaaaa tagatcttga aaaaaagagg ctgatgcgtt
     1381 ttcgcatcag cctctttagt attaaaaata aaaatgtaaa acgcagaagt ataaccctta
     1441 actgacttgc aaagcaaggt taaggagcta tactaaattt ttcagaaatc agattatttc
     1501 ttcagcagat ctctgatttc ggtaagtaat tcttgttctt tcgatggagc cggtggagca
     1561 gcaggagctt cttccttttt cttcttcatg ctgttcattc cttttatcat caggaaaata
     1621 acaaaagcaa tgatgatgaa gtcgatgact acctgaacaa aattgccgta atttaacgta
     1681 accgcagctt tttcggctgt tgcttcgcta atagtaacct tgaggtcggt gaagttgacg
     1741 ccacccaata aataaccaat gggcggcata attacgtcgt taaccagtga gctcactatt
     1801 ttaccaaatg ccccaccgat gatgataccg acagccatgt cgataacatt gccacgcatg
     1861 gcaaaatctt tgaattcttt tacgattccc atgattaagt ttttaagtta ttactagaac
     1921 ttatacgtaa atccaatacc cagtatttcc ttgaattgca gtctcgggcc ccctggacca
     1981 tcagttgcat taattactcc gtcgtcgtta cggtcaacgg caatgataat atcgtcgtcg
     2041 tacatgagct gggtggtaat tgtggccgaa atgaatttgt tcaccttgaa tgagaaaatg
     2101 ttttcccagt ttacatcaat attctgagga ttgtgcagat agttcgagaa taagtcgagt
     2161 ttcgataata cggttacatt tttttctttg aaaaatgaat gggtataaac aactctgata
     2221 tagcccccga attcacttct cactttctta ccaggggtag tgagcaagcc atctgtgtaa
     2281 atagcaggtg tcaccccaaa agctccggca ttggcaaagt cttcgtcgag aacaaaggtt
     2341 gttttcgatg taaaaggtgc aataaaggca gtgaagtcgg cgcatggctt aaaatccatc
     2401 ccgatggctc caatcagata ggcaggggcc attagcttcg aaatagcaac agaatcattc
     2461 gggtagttgt agccatttgt catttgggtt ttaaaattca aaatagcggc ataataccac
     2521 gatttcgatg cttgtcttcc gtattttgat gaaaagtcaa ttttatcgtc ggtttttata
     2581 aaactgctgt ttttgccttg tttcaatatc ccatagccca catcgagcat attgtcccat
     2641 gacgatttgc ctttgtgata ggtggcaaaa gcactgaaca gtccgttgat gcccaacgaa
     2701 ttctgaccgc cggcagccca attgacaaac gaagcctgag ccaagttgat gcttagaact
     2761 cctccggttt tccacccatc atcagtggta gtatttgcat tgcgcaaggt tttttctcca
     2821 tcggtaacct gagcgataac cagattaaaa ttaagcacaa ccaacaaaat tagtagtagt
     2881 tttcttttca tttttttaaa ctattaaagt taaccagtat actttttatg taatttaaga
     2941 ttactattac aaatataaaa atggtttttg tacttacaaa attataaatt ataattgttt
     3001 tgtcagcgtt gtgcttcagc agatagtgta gcctatgata ccgatatgtt ttacatacta
     3061 ctaattttta gaaggataat tcttataccg gattgcaatc agtacgttaa aacaattcgg
     3121 aaaaacctat aacaatgaat gcctgagcca taacataagt tatcataaag tatactcctg
     3181 caaagcgttt tggctttctg aaattatgga gggccaaaat agaatcggaa ataataaaaa
     3241 agatgctgcc ggtaaaaaca aagccggtgg tataactaca attgaaaatg aacaacaaca
     3301 ttgaaagaaa agacattccc aataagaaag acatataaac aattaccggt atttttaatt
     3361 ttcccaatcc ctttttaagc aaattgaaaa tacaaatgcc tattacaaaa taaataatta
     3421 ctgacgtaaa tgctatcgca ggaattttgt gaaacactat taaccattgt aaaatgccat
     3481 atatataaag cccatgtgct attaaaaaag caatcaatcc gaaaaggaag catttgttgt
     3541 ttttaggcca tagtaaaaaa atgtctcctc caaaagaaaa cccaagcgca gcaatgatta
     3601 tagggctgaa attatgggta ataaaggtat atagtaaagc catcactggc atgataaagg
     3661 gtttggttac atatcgcagc ttttgccaat cggttgcctc tgctgtaatt atgccaataa
     3721 atgcaattgc aagcagaatt atcagaaaaa cgatgttcat atattgcaat ttgaatactc
     3781 taagataaat aaaattttga taaagaaacc cgatgagctg taagatttgt taaaatcata
     3841 aaaaaagcag caataacaaa gccttgctgc ttttaggtcg gggtgagaag actcgaactt
     3901 ccggcctcta cgtcccgaac gtagcgcgct accaactgcg ccacaccccg aaattcaagc
     3961 tgcaaaatta gtattttttc gatacagata attatgcatg aaataaaatt cattggtcaa
     4021 ttttattaat taaaaactaa gccgaaaatc atttcttgaa aacataataa attagtaatt
     4081 ttaaaacaga tttttaccct ccaaaaatgc ccttaatatg agcagatcaa ataaaaaaac
     4141 taccggaaag cagcgcagca acggaacatc tgcacaggaa atcgggtgga gccgtaagat
     4201 ggaatcggtg ttgttggcga attcggacct tacttttgtt attgataaaa acggggtttt
     4261 taaggagctt cactattccg atccatcact tctttatgtg cagcctgata atatcatcgg
     4321 gatgaaagtt gaccaattgt tgccgggaga tgttgctgaa ataacaatgc ataagatagg
     4381 tcgtgtgatt gaaactgata aaaaggaaca atatgtatat gccctgggtg ggcatacttt
     4441 tcgatccaca attaccaaaa ttgacgatga cacagtaatt gcactgatta ataatattga
     4501 ggaagagatt ggccttcgcg agaacctgaa tttaaccaaa cagaattacc gaaagctgat
     4561 tgaaaacatg ccgttgggaa ttgtgatatg cagaatgata tatgatgaca ataacaaatg
     4621 catcgatttt aatgttgtgt cggtaaacaa ggaattcatt attataacca acgaaaccga
     4681 tatagttggt aaacaactta gcaatgtgtt ttcggagatt gaatcacagt ggtttgcgtt
     4741 gtgcctcaaa gcggcaaatt cgggcaaggc tgtcagtcac gaaagctatg acccgtggat
     4801 ggataaatgg atttcaatga caggttaccc aaccgggcag gaagagtttg tgatggttat
     4861 gactgatttg gggaaatcgg tggaacagcg gtatctgata cgaaaaaatg aggagcgata
     4921 cagttttgca ttaaagaatt ccaaagcttt tgtgtgggaa tggaaccggc aaatggaaat
     4981 gagctatttt tcggaaagcc tttttagtat tttgggatat gttaaaaccg acctgccacg
     5041 taccttcaga tcggttcttg atatgctgca tcctgatgat gttggtaccc tgaagaacgt
     5101 ttttgctgaa cttcataata gggtaaatga tcagttcagt gtgcagctgc gtttcagaaa
     5161 gaagaacgga gaatatgtct ggctcgaaac tcagggtaag gttgccgagt ttgaagcaaa
     5221 cgatctgcct taccggataa ttggaagttg ccttgacatt acctccatta aagcaaaaga
     5281 aatcaggctt cagattagcg aaactatttt caaaagcatt ttcaataatt ctccgattgg
     5341 tatcggattg ttcgatctgg ccggaaacct tattcttgca aatgaagcag ctattaaaat
     5401 atttaaaata accgagaatc cggaagctaa aaaactaaat atttttaccg gtaatatcat
     5461 tcctgctgaa tacctgaatc aggtgaaaaa tctgaaacca gttgattttt ctgcttggtt
     5521 tcgcaccgaa aagttgttgt tgcaaggtat tgagataaca gagaaaaaac gggttttcct
     5581 gcattgccgg tttacgccat acaacattaa tctagaatcg aataatgttg acagattact
     5641 gcttcaggta actgataaca catatgagaa acaacacgaa cgggaactgc tgatatctaa
     5701 aaaatatcat gaaaatgcca tggagattgc ccggcttgct ttttggagct atttcttcga
     5761 tgaaaagaaa ctaaatattt cttcggaatt tatgcacatc ttcgaattat ccaacagcaa
     5821 tgatattgag aatatagaga ttatgaaaag aataatctca ccggatgatt gggagttttt
     5881 agttcagttt tataatgatg tggtttcgca aagtattgaa aaagaagctg aatacgaatt
     5941 cattattgtt acgcagtcag gagaaaaaca tgttttttcg aaagtcgcag ttatttacga
     6001 gaagggcaat aaacccattg gtattttcgg agtactacaa gatatcacgg aacgtaaaaa
     6061 atatgaaata gagctgaaga aagccaaaga aagtgctgaa gaaagcgatc gcctgaaaac
     6121 cgcgtttctg gcaaatatga gtcatgaaat cagaaccccc ttgaattcga ttgtagggtt
     6181 ttcgagtttg ctggcaaacg atatgttcga taatgctgaa cgtacggagt tcgcaaaacg
     6241 cattgaattt aacagcaatc agttgctgaa acttatttca gatattattg atatcgccaa
     6301 gatagaatcc ggttctctca cagtaatctg ggaacccgtt ttactgctgg agttactgca
     6361 aaatgtttat catgccaata aaattaactg cccagagaat gttagttttt ttgtcgactt
     6421 taagggaatt gaacctgatt tgcaggtgca gaccgatttg gtgaaattta cccagataat
     6481 tacaaacctg ctgagcaatg cttttaaatt tacagagaaa ggtaaaattg aatttagtgc
     6541 aacgatttta aataataata tccgtattaa tatttgcgat acaggaattg gaatcaggca
     6601 ggaagagctt gacatgattt tcgaccgatt ttatcaggcc aatcatttta aaacgggaac
     6661 cggtctggga ctgtcgatat gcaaaacact taccgaagca atgggtggta ctatcgaagt
     6721 tacgtcaacc ttgcatgaag gtagtaggtt tacggtaaat ctgcctctta aaatctcatg
     6781 atgaaaaaaa cgatttttgc tgtaatttta agtattgtta tattctcgca atatgtagca
     6841 gcacaaggcg cctataatgg cgttgtggtt gtctgcgaca tcgacgatac gtataaaata
     6901 acaaatacga catcgaagtt tagctcggtg attaacgcct tgttttcttc ccgtactttc
     6961 gggggtatga atgagctact tttaggaatg aatgcacggg gtgcacaaat ttattttctt
     7021 tccaactcgc ctaaagtttt aaaacgaaga atcattaagt tactcaagga aaatcagatt
     7081 gaattcggtg gattattttt gcgaaaaacg ggagaagatg gcatcagtca taaaattact
     7141 gtactcacat caatgattga tacatgttcc cgacaaagtt ttatcctgat tggggatgac
     7201 agtgagaatg accctttaat ttacgattcc ataaagcact tgtaccccga aaatattgag
     7261 gcaatttata ttcatcatat taaagggaaa aatctccgga ctttctaccg cttttattct
     7321 gcggtcgata ttgcttatta tgaatcaatc gtaggcagaa tgaatgatgt tggtacggaa
     7381 aagatactcg acgccgtaat ggcatctgaa ctacacagtg tttttcccga ttatattgtt
     7441 ttcgacgaaa agttgattct cagtgatgtt gcaggctgtt tcgatcgaca ctgtctgagc
     7501 aaaaacaaaa ctattgagtg gttaatagat tgttggtata aacagtaggt acgagttttg
     7561 tattatgtta atttttgtaa ttttgaaccg caaaacaacg tatcatgaaa aaactgttag
     7621 tcgttattgt atgtgcaatt ctttctttgc cggtttttgc tcaggaagtt gatatatcca
     7681 gcgaaaaggt acttattgat ggtaaaaagt tttacctgca taccgttaag caggggcaga
     7741 cattatatgg aataagcaaa gcgtataatg tatcgacaga tgatatcatt cgcattaatc
     7801 ctgaatcatc caatggtgtg aaaaccggtc agatactcaa agtgcctttt aatgaagtaa
     7861 gcaacaatga acctgtatcg ggaaacccgg ggaaagataa agaaactcat catgtaagta
     7921 atccgattcc cgaaggagca ataacgcata aagttgagaa gaaagaaacc atttacagta
     7981 tctgtaaaaa atataattta tcggttgagg ctttgtataa agccaaccct tcaatacagg
     8041 agaaaggctt gcgggctgga gatgtgctgg ttattcctga atcggattta ataaccgatg
     8101 caaccaaacc ggaaccggaa gtacagaaac cggaaccgga gaaccctaaa gatgacagca
     8161 ttggttttgt taatcacatc attgtaaaag gagaaacctt atcggcaatt tcgaaaaaat
     8221 atggtgttac tattgctgaa atcctaaaat ataaccccgg tgtttctgcc gataaaatta
     8281 aagagggcga taaattgcaa attccgcgta aaccaaacga acaagcagca ttccctccag
     8341 agcaaaggaa tcataatggg cagggtggcg acaccctgaa cgccaaagat tcgcgatcga
     8401 ttgagtataa aattaagaac gaaatcaaca taatcatgat gctcccattc gaagtgtacg
     8461 taaacacaaa gaactggtac aatcaggaac tggggagtaa agaatataca gtgttgccgg
     8521 taaccgaaat gatgctatcg tattacggag gttgtcttat ggctttcgat agtattaaac
     8581 aaaccggact taaagtgaat atgcatgttt ttgataccgg atctgatact tcatcgctcg
     8641 gaagcctggt gaataaagat atttttgcaa attccgatct tattatcgga ccggtgtatt
     8701 caaaacagat aaattatatt aaagatttta tacccgaaac cgctgtattg cttagccctt
     8761 tttccgatta cgaaaaagtt gatattggcg ataaatcggt gttatgggca aacccgggaa
     8821 aaatagggcg ttttgaggct ctggctgcct ttgcggcaaa aaatgcttca aacaagtaca
     8881 ttatcatcta caataacgat ggcaaatcga aagaagatgc agaggagctg cagaaaatga
     8941 tgaatgaaag ctttgctttg gcaggtaaaa aggacagcct gaatgtttcg gtgctttcat
     9001 catccgaagc aaatggtgca agcttaggaa aattgttatc tcattcagga ctaaatgtta
     9061 ttatttgcga tgataagagc gaagcccagt tatcgagcct gatggcacag cttgtacaaa
     9121 ttaaagatgt ggatatacaa ttgattggag aagaatcctg gctcaattac cgtagcattg
     9181 atgtaaatta ctttcagaaa ttaagattca gttataccac accatattat attgattaca
     9241 ccaacgataa aaccctgaga tttatcatag cttaccgtca gattttcctg agcgaacctg
     9301 atgagttttc ttttgcagca tacgatcaga tgctcatgtt ttcgtcatta tatatgaaac
     9361 atggcggcaa tcttgcagac gcatttaata tgaaagtgcc atattgtggg atggtatcaa
     9421 aaaacaactt tgtaaatatt tccgggtcaa gacaatatgt gcagagtttc tgtaacctcc
     9481 tgatggtgga tgaagacttt cagataaaaa gaatacatcc ctaaagcctg ttttgttaat
     9541 cggtttttgt tatttaccaa aataattttt acttttaacc ggtaattgat ttttgaagca
     9601 tgttattcag gcaactaaat gttattttgg tcgtcaatta gtaacttata agtaaattat
     9661 ttgataacac aaatacacct aaaatatgat gagaaaagca ggtaggttaa gtgccatcat
     9721 ggtatttgtc atgattttct gtattttttt aacacaacca cgtgtacaag catgtaatta
     9781 ctcctctact tttgcaggca ccaccataac cccaactgcc acctggcaaa cagcaggttc
     9841 ggtgcaggca ggggcgcgat atcagtttac tgcgtcgcaa tatatggtat atgtattttc
     9901 gtattgcgat ggagggggtt ccaattcagg cgatacccaa atagaaatat ttagtgctgc
     9961 cggcgtttcg atggctgaaa acgatgactt ctgcacgttg ggttcaaagc ttacctgggt
    10021 agctccggca agtggaacct attatgttgt aacttttaaa tacaattgcc ttgcagacca
    10081 tacggttatg gggacaatgg cttataaagt catgacccag ccaaataatc aggattgctt
    10141 gggtgcaata ccactctgtt tcaataccta ttccactaca atttcatata gcggtgttgg
    10201 aaattacacg ggcgaaattc cgacaaccgg agggtgcccc ggaaattgtt tgttaagtgg
    10261 tgaaagaaac gatgtgtggt acacttttac ggtgcagtca agcggtacgg ttagtttttt
    10321 aatagaccct gttgacaacg acgatgatta cgactgggca gtatataacc ttacgaataa
    10381 tgagtgcgct actattgctt cgaatccttc atcggtgcag gttagttgta attatactgc
    10441 cacagcgggg aatacaggtc ccaatggagg aagtaccaac acctgtattg gtgccagcgg
    10501 tactccctac aatgctgcct tgaatgttac tgccgggcaa acctatgtag tgaatgtgag
    10561 caatttttca tctacccaaa acggatactc tattactttt ggggggagtg cgcagatact
    10621 cgataacagc gacccgtttc ttacaggtat agtgtatacc ccggtttgcg gacagaataa
    10681 tattaccgtt cagttctccg aaaatgtaac ctgcctgtcg atttccccat cctatttctt
    10741 tgttaccgga ccagggggaa cctatacaat cagcgatgta gttagttcaa tttgtgctgc
    10801 aaatggctca tatgatgata cctatactct tatgttgtca gaccctcttt ccgatggcgg
    10861 aacctatacc gtaggattaa caagtaccaa tggtattaat gatatatgtt ataatcaggc
    10921 cgtgaataat ggtaatttta cttttagttt tacaggcctt accgcttctg gatcggttac
    10981 ccataatgta acatgctatg gaggaaccga tggtgcagca actgtaactt ctgtgagcgg
    11041 cggaacacct ccttacgatt acacatggtc gaatggtgca acaacacaat ctgttgccaa
    11101 tttaccgggt ggtgtagctt acgttacggt gagcgatttc tacgattgcc aggaagtgct
    11161 cacttttaat attacgcaac cttctcagat tattgttgaa gcgggtacaa gccagcctgt
    11221 ttgtccgggc aacggtatta ctctcggggg atcaccaacc ataacgaatg gggtcccacc
    11281 acttacgtat gggtggtcgc ccacaacc
//
LOCUS       DAOOQP010000096        11281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7213_length_11281_cov_4.484678, whole genome shotgun sequence.
ACCESSION   DAOOQP010000096 DAOOQP010000000
VERSION     DAOOQP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7213_length_11281_cov_4.484678"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2727)
                     /locus_tag="PLK75_09680"
     CDS             complement(<1..2727)
                     /locus_tag="PLK75_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOY39556.1"
                     /translation="MKRFFTIILITTLTFSAYSQNNDLTHQMWYSSYDQNMWGPDWAY
                     GIDVEQPIFDVNIDESWGFTEVTDILGMQLGVGFEVGIQAILASTFEAHGFYTGEFDL
                     DYPIETHLDFPEDYTFDYGGPATIYTDYEVTDGWNLETEFPPVGVITLDFEYMFNPDM
                     NIIVCVFGCDTIELIPAAVALPYTLDTLFHINGETQYCIFPCYVDGQFQFCHDWELPI
                     DVDFTDLVGFNFEAHITLPYVETEDHIELESNCLIAQGDSMYLYVHLDVLGFLYVMAG
                     YIPAPEGPQIQEAIDFLNDTISYPIETPLGEVIFQIEYSLLQVNLLLRNYLHQDIYFC
                     PTIWGTFSFPIPLEYVVNNPDDGSTVETGFNDSVRMAIGNDLTITYPCHGWDSMYVAV
                     QYNIQPTIRNHTWDSMAFALLIEALEVNITIITPFKATYGDAEMPEFKLPTYISGNAD
                     LPEPIKCPPIEMSSYYAYNEDDPKDFGPWTIGPLFVWEIPLGYIPLTWFDETWELEHF
                     QEDIVFPGTYIKPYDKSEVNALLYASSYCYGDPYSYVHAEAQNGVPPFTYTWSTGDIT
                     YNTYSDVDSIFAPPGYYLVTITDAYGCESYDSISIVVDPPLNYDLEVTDVLCHGMHTG
                     IVYTNVSGGTPPYFYHWEPSGSEEANPSNLAAGWHYVTISDWVGCPIIDSVFIDEPET
                     ALSFNTLVTDVQCNGLTNGSIDLNPTGATPPYNYIWSNGATTQDLLNIPAGAYNVTVI
                     DANNCNWMDLYVVNEPDTLIAYLMHTDITCYGDDDGTLDVITTGGVEPYTYTWFHDPS
                     NHNSSLTNLPPGFYMVSVTDSHNCTDTALSMITQPDELIINSNIFNLNCFGIPDGMIV
                     VEPVGGTPDYIIHWSNNYFNDTIQNLFPGTYTVTVTDEHNCSIIESYTVTQPS"
     gene            complement(2738..3253)
                     /locus_tag="PLK75_09685"
     CDS             complement(2738..3253)
                     /locus_tag="PLK75_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39557.1"
                     /translation="MSKILLTFVSFIIVWSVSFSQTLTSFVEEKQANMVYDTAKLLQV
                     PNTSVKMIPPEYFIPDENIHGFAHPGSACTIQVIEIPGVSHQTIESAMTDEYISSQGY
                     KLIEKKVVQTKTGERAAWFVVQFNTKDVVYERIMLFTGSSNTIWVNANYPQELKKLLL
                     PALEAALLSVQ"
     gene            complement(3573..4226)
                     /locus_tag="PLK75_09690"
     CDS             complement(3573..4226)
                     /locus_tag="PLK75_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY39558.1"
                     /translation="MIAATQITKSFGALEVIKGIDLSVETGEIISIVGASGAGKTTLL
                     QILGTLLKPDTGKIYIDGNDISKLGDKAMAALRNEKIGFVFQFHHLLPEFTALENICI
                     PAFIQGKSKAQSEKAAMELLEILRLSDRINHKPNELSGGEKQRVAIARALINKPSVVL
                     ADEPSGNLDSRNAEELYKILFELRHEYHQTFIIVTHNEHHAKITDRTYTIVDGKIIQ"
     gene            4398..5948
                     /gene="dnaB"
                     /locus_tag="PLK75_09695"
     CDS             4398..5948
                     /gene="dnaB"
                     /locus_tag="PLK75_09695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOY39559.1"
                     /translation="MANNSAPYKKNITQINEEYGRIPPQAVDLEEAVLGALLIEPDAI
                     YSVVQLLTPDSFYKMEHRTIFESILNLYKNHQPIDLLTVTEYLRKTSKLEEVGGPFYI
                     SSLTNRIGTSAHIEYHSRIIQQKYIQRELIRVSSEIQQKAFDDSLDVDKLLNFSESEI
                     FKISEGHLSRESLKISQLIPDVMQGIEEASKREDKLLGIPSGFSDLDRLTAGWQPGDL
                     VIVAARPSMGKTAFVLSMARNMAVTHKKEVAIFSLEMANAQLVHRLISIEAELPGDKL
                     KTGNLRQYEWKQLESKTKNLENAPIFVDDTPAITIFELKSKCRRLVQHRNIKAVFIDY
                     LQLMTTGMDMKGSREQEVSTISRSLKAIAKELGVPVIALSQLNRAVEVRTGDKRPHLS
                     DLRESGAIEQDADMVIFIHRPEYYNQTEDRDGRSTVGLAEIIIAKHRNGPTGVINLRF
                     KSEYAKFTDPEVVIAGVNAINDGNTIKVSSKINTDEIKNHTDVSFSDGKEYDHDSGDK
                     GNNRDSPF"
     gene            6039..6503
                     /locus_tag="PLK75_09700"
     CDS             6039..6503
                     /locus_tag="PLK75_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39560.1"
                     /translation="MKIVSAFVFLMAALSSKAQCPDSMPLWLLGKWQIQNESGLSFEE
                     WTLSNDTLMVGRTYSFYGNDTLELDRMRIVCSQGSVVFYMNASVNNNNIVVPFFPDKD
                     NNSKVWAFYNPDAAFPHTFRYVYASADECFVSFESNNPLEACTDFKMIRISR"
     gene            6504..6956
                     /locus_tag="PLK75_09705"
     CDS             6504..6956
                     /locus_tag="PLK75_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOY39561.1"
                     /translation="MQALNFPSYQFSTRIVGERTEIFDQFRKKYVVLTPEEWVRQHLL
                     RFLVEERSYPPGLISVEKSIDVNGLQRRYDAVVFDRSGKPLMIIECKAPSVAVTQQVF
                     DQVYAYSMNIPASFYFISNGIIHAVVKKAQGSAAAFLDDIPSYIEINS"
     gene            complement(6953..7579)
                     /locus_tag="PLK75_09710"
     CDS             complement(6953..7579)
                     /locus_tag="PLK75_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY39562.1"
                     /translation="MKKLITTFGLLLLAVVIFAQGFHVGPRAGVTVSNMITKFNDGTV
                     EYASKPGFQVGATAEFELTSFLYVGTTIGLYQKGYKNVVTPGVFTIRNTFSYIDIPIE
                     VGYKLPIGNFSIFGQIGPYVSVAVVGQSRMVNHDMPEYNETYSVYEYDEYKRFDSGLS
                     FAVGGDYKQFQIRANYAFGFIDMNKYETYSSVNSTFGFSFAYFFGRDY"
     gene            7710..8498
                     /locus_tag="PLK75_09715"
     CDS             7710..8498
                     /locus_tag="PLK75_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39563.1"
                     /translation="MRLPVVIILLLLSISAMPQKLIRGYTVGYAHGFLSHGDAISIID
                     TTGYVDESYHAYADEEKLKYASASSLYFGFPIEYGYSRHRFTLLPAVDINTAHWYLHF
                     EDPESMPFGLSANDSLYFKSFMIMPQVGLTYKFHFFAGGIHLAVGAGVDVKIPLSILT
                     QTSLVDSDKTEYDPTMLGYKIHYTPNTIFDIASNPGVHLSPKFVFDIYLSRFLAINLM
                     YYRNKLNSGEHNPLLSGYFGGGVTYLLPFGKEDDIRVLQQYKKE"
     gene            8568..>11281
                     /locus_tag="PLK75_09720"
     CDS             8568..>11281
                     /locus_tag="PLK75_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY39564.1"
                     /translation="MAKSMIIAFFLTIIVVGSNAQNVIPVVQTGHSGKIKCVNWDNTG
                     RYIASADMNNDVVINDVIAGKQFFKVKLPGKGTVTGLQFDKSKLIIANHGQNYVFDMN
                     SLTLEPCNESINIAPDDGGYKLRGATVKKGARSLINKDIYVHFTQVASHPNGELVIAG
                     DEAGWLYFCKPSLNIIKRDRTHRLDITDIDYSDDGKYIAIASADRSISIWSIAELKLE
                     KRLVPRSFNIYGMDAHPDGSRLAFGDELGYTYLLVFENDKITCKGINTHRSRVNDVCF
                     TPNPMCVATAGGDNFASVVDFENNIPLQYFKLQPNTSKLKQTFNIKGIQAKATSTDED
                     EIFFDENVYSVAVSPDGKQIAYSGGKWGNTNPMLKVSAISNLNLDEPKEKRKKRKMEI
                     GLTQKNNKHIFPQLFFISENEFYGLGESNNEAQRFKLSITGYDEKDNTSVRSGFDDNV
                     RPNNMTVIVSKSKKSMTDDYLVKKDLNSEDEFSCRGYTITRTSRGRSVKFEGHRGYIN
                     DVEIIRDKNYLISSAEDAAMIIWNIETGEKIMTVYVVDQGKLVFISPDNYYLAQGDAL
                     TGMGFNYNGRVLPAEQFDLKYNRPDIVMDKLGIFKPDVIALYYKAYLKRLSKTGFNVE
                     MLKADMNLPELVIENVTDIQYKTTQPDLKLKLNMSDKMYEIDRMNVWVNDVPLFGTNG
                     LSLIDKHTKNTGTEVNIPLSEGNNVIQVSCMNSIGVESLKDEFVIEREVSKKRQPDIY
                     LVSIAVSDFKESKYNLRYTVNDGKEFIKLFEKRGGNYGHVYVDSFYNASCTRENVLNA
                     KNRLMNSHVDDYVIVHIAGHGLLDDNLDFYFATSDIDFSNPSERGLRYDELEGLLDGI
                     PARNKLFLMDACHSGEIDKGDDFAVEQVIGDNQRGVTVFTPQGNTVPKT"
BASE COUNT         3392 a         2169 c         2382 g         3338 t
ORIGIN      
        1 gctgggttgg gtcaccgtat aagattctat tatactgcaa ttgtgctcgt cagtaacggt
       61 aacagtatat gtacccggga ataggttctg tattgtatcg ttaaaataat tattactcca
      121 gtgaataata taatctggag ttccaccaac gggttcaaca actatcattc catcgggaat
      181 tccaaaacaa ttcagattaa aaatgttgct gttaataatt aattcgtcgg gttgtgtaat
      241 catgctcaag gcggtatcgg tacaattgtg cgaatcggta actgatacca tgtagaatcc
      301 cggaggcaga ttggtaaggc ttgaattatg gttagaagga tcgtggaacc atgtatatgt
      361 gtatggttca actccgccag tggttataac atcaagtgtg ccatcgtcat caccataaca
      421 ggtaatatcg gtgtgcatca gataagcaat cagggtatca ggttcattga caacatataa
      481 atccatccag ttacagttgt tggcatctat cactgttacg ttataggccc ctgccggaat
      541 gtttaataaa tcctgagttg ttgctccgtt gctccaaatg taattgtaag gaggggttgc
      601 ccctgttgga ttaaggtcaa tactaccgtt ggtgagccca ttgcattgca catcggtgac
      661 caaagtatta aaacttagag ctgtttcggg ctcgtcgatg aaaacggaat cgatgattgg
      721 gcatcctacc cagtcggaaa tagttacata gtgccagcct gctgcaaggt tacttgggtt
      781 tgcttcctcg cttccgctgg gctcccaatg atagaaatag ggtggagtac ctccgcttac
      841 gtttgtgtaa acaattccgg tatgcatacc atgacaaaga acatcggtaa cttctaaatc
      901 ataatttaaa ggtggatcta ccacaatcga aatagagtcg taactttcgc agccataagc
      961 atcggtaatt gttaccagat aatatccggg aggtgcaaaa atactgtcaa cgtcagaata
     1021 agtattgtag gtgatatcgc ctgtagacca tgtataagta aaaggaggaa cgccgttttg
     1081 agcttcggca tgtacatagc tgtaagggtc accataacaa tatgatgaag catacagcaa
     1141 tgcattaact tccgatttat catagggttt gatataagtt cccggaaaaa caatgtcttc
     1201 ctggaagtgc tccagttccc atgtttcgtc aaaccatgtc agtggtatat agcccaaagg
     1261 aatctcccaa acgaacaaag gccctattgt ccagggtccg aaatccttcg gatcatcttc
     1321 gttgtaagcg tagtaactgc tcatctcaat gggtggacac tttataggct caggtaggtc
     1381 ggcattgcca gaaatataag ttggaagttt gaattcaggc atttcggcat caccatatgt
     1441 cgctttaaaa ggtgtgataa tagtgatatt tacttcgagc gcttctataa gcagagcgaa
     1501 agccatactg tcccaggtat gattgcgtat tgtgggctgt atgttgtatt gcaccgcgac
     1561 gtacatacta tcccatccat ggcagggata ggttatagta aggtcattcc cgatagccat
     1621 tctaaccgag tcgttaaacc ctgtttcaac agtagaaccg tcatcggggt tattgacgac
     1681 atattccaat ggaatcggga aactgaatgt tccccagatt gtaggacaga aatagatatc
     1741 ctgatgcaaa tagtttcgta ataaaagatt tacctgaagc aggctgtact cgatctggaa
     1801 aattacctca cctagcggag tctcgatagg atatgatatt gtgtcgttta aaaaatcgat
     1861 agcttcctgt atttgtggcc cttccggcgc aggaatatag ccagccataa catataaaaa
     1921 cccaagtaca tcgagatgaa cgtataaata catgctatcg ccctgtgcta tcaaacagtt
     1981 ggattccaac tcgatatggt cttcggtttc gacatagggt aatgttatat gggcttcgaa
     2041 gttaaatcct acaaggtcgg taaaatcaac gtcgatgggc aactcccaat cgtgacagaa
     2101 ttgaaactgc ccgtcgacat aacatggaaa aatacaatat tgtgtttcac cgttaatatg
     2161 gaacaatgtg tcgagggtgt atggaagcgc aacggcagca ggtattaatt cgatggtatc
     2221 gcatccgaaa acacaaacga taatattcat atccggatta aacatgtatt caaagtccag
     2281 agtaattact ccaaccggag gaaattcagt ttcgagattc cagccgtcgg taacttcgta
     2341 atcggtgtat attgttgcag gacctccata gtcgaaagtg tagtcttcag ggaaatcgag
     2401 gtgggtttct atagggtaat caaggtcgaa ttctccggta tagaagccat gtgcttcgaa
     2461 agttgaggca agaatagcct gaatgccaac ttcgaatcct acccccagtt gcattccgag
     2521 tatgtcggta acttcggtaa atccccagct ttcgtcgata ttgacatcaa atatgggctg
     2581 ctcaacatcg attccgtaag cccagtcagg tccccacatg ttctgatcgt acgacgaata
     2641 ccacatttgg tgggtaaggt cgttgttttg ggaataggct gagaacgtca gcgttgtaat
     2701 tagtataatt gtaaaaaatc ttttcataaa taacaattta ttgaaccgat agtaacgctg
     2761 cttccaatgc cggtagcaat aatttcttaa gctcctgggg gtagttggca tttacccaga
     2821 ttgtattact gctccccgta aaaagcatga ttctttcata tacaacgtcc ttagtgttga
     2881 actgaacaac aaaccatgct gcacgctctc ctgtttttgt ttgtacaact ttcttttcga
     2941 ttaatttgta tccttgcgaa gagatatatt cgtcagtcat tgcactttcg atagtttggt
     3001 gcgacactcc gggtatttca attacctgaa ttgtacaggc agatccgggg tgggcaaaac
     3061 catgaatatt ttcatcagga ataaagtatt ccggaggtat catttttacc gaagtattag
     3121 gaacctgcaa tagtttagct gtgtcgtaca ccatgttcgc ctgcttctct tcaacaaaac
     3181 ttgttagtgt ttgcgaaaac gaaacactcc aaactataat aaagcttaca aatgttaaca
     3241 aaatctttga catgactaca aaattttaag actgctaata tagaaaaact attgcattta
     3301 acgcaatttt atgacgttat ttactggatt tagttgtctt tatttattca cagttttttc
     3361 ggattaaccc caagattttt acgttaacac tttctctttt accaagataa caaaaacaag
     3421 gcaagcatca agtttcgata aattgttgat aaaataataa tctacagtgt gcaactacct
     3481 tccacgaggc aaattgctca agttttatga tttattgtgg tattacaaaa acaaattatc
     3541 atggagcatt tataacctaa tttttataag cgtcattgaa ttattttccc atcgacaatt
     3601 gtataggttc tgtcggttat tttagcatga tgttcgttat gcgttacaat aataaatgtc
     3661 tgatgatatt cgtgccggag ctcaaataaa atcttatata gctcttctgc atttcgggaa
     3721 tcgaggttcc ctgaaggttc atcagcgaga accaccgatg gtttgttaat cagggctctc
     3781 gcaatggcaa cgcgttgctt ttcacctccc gaaagttcat tgggtttgtg attgattctg
     3841 tcggataacc tcaatatttc aagtagttcc atagccgctt tctcgctttg tgctttgctt
     3901 ttaccctgaa taaaagcagg tatgcaaatg ttttccagag cagtaaactc tggtagcagg
     3961 tggtgaaact gaaaaacaaa cccgattttt tcattcctta atgctgccat agctttgtcg
     4021 cctagtttag aaatatcgtt gccatcgata taaattttac ctgtatcagg tttcagtaag
     4081 gttccaagaa tctgcagaag cgtagttttt ccggcaccgc ttgcgccaac aatcgaaatg
     4141 atttcaccgg tttcgaccga taaatcaatc ccttttatca cttccagtgc cccaaacgat
     4201 ttggttattt gtgttgctgc aatcatatcg caataattat gcgctaaaaa taaacatttt
     4261 ttcacaatca tgtttgataa cttgtcagct agttgtatgc aaacatgtta aactgttgaa
     4321 aactataatt caagtaaaac agtgaatgaa gatatcttga cttatttttg tttcctgttt
     4381 ttatttaatt cacgtttatg gctaataact ctgcaccgta caaaaagaac attacccaga
     4441 taaatgaaga atacggccgc attcctcctc aggctgttga tcttgaagag gcggttcttg
     4501 gagcattact gattgagcct gatgctattt actctgttgt tcaattactt acgccggata
     4561 gtttctataa gatggaacat cgcaccatct ttgagtcgat actaaatctg tataaaaatc
     4621 accagcctat cgaccttctt acagtgaccg aataccttcg aaaaacttcg aaactggaag
     4681 aagttggtgg tccgttttat atctcctcgc ttaccaaccg tattggcact tcagcacata
     4741 ttgagtatca ctcccgcatt attcagcaaa aatatattca acgagaactg ataagggttt
     4801 cttccgaaat acagcagaaa gccttcgacg acagcctcga tgtggacaaa ttgttgaatt
     4861 tttcggaaag tgaaattttc aaaatttccg aaggccatct gagtcgggag tctctcaaga
     4921 taagtcagct tatacctgat gttatgcaag gtatcgaaga ggcgagtaag cgtgaggata
     4981 aactgttggg tattccttcc ggcttttctg acctcgaccg tttaaccgca ggatggcaac
     5041 ccggcgatct ggtaatcgtt gctgcacgcc cgtcgatggg taaaactgct tttgtgctca
     5101 gtatggcgcg caatatggct gtaacccaca aaaaggaagt tgccatattt tcgctggaga
     5161 tggcaaatgc gcagctggtc caccgtttga tatccattga agctgaattg ccgggcgata
     5221 agcttaaaac aggaaacctt aggcaatatg aatggaaaca gcttgaatcg aagactaaaa
     5281 atcttgaaaa tgcccccatt tttgttgatg atactcctgc aattactata tttgaattga
     5341 aaagtaagtg ccggcggttg gtgcagcacc gaaacattaa agcagtattt attgattatc
     5401 tgcagctcat gacaaccgga atggacatga aaggcagccg tgagcaggag gtaagtacca
     5461 taagccgctc gctgaaagcc atagctaaag agcttggtgt tccggtaatt gctctttctc
     5521 agttaaacag ggctgtagag gttcgtaccg gagacaagcg accgcattta tccgaccttc
     5581 gtgaatcggg tgctattgaa caggatgccg atatggtaat atttatccat cgcccagagt
     5641 attacaatca gaccgaagac agagatggtc gctcaaccgt tggactggca gaaattatta
     5701 ttgctaaaca ccgtaatgga ccgactgggg ttataaacct cagattcaaa agtgagtatg
     5761 ccaagtttac cgacccggag gtggttattg ctggagttaa tgctatcaac gatggcaata
     5821 ccataaaagt ttcttcgaaa attaatactg atgaaatcaa aaatcatacg gatgtttctt
     5881 tttcagatgg taaagagtat gaccatgatt ccggtgataa agggaataac cgtgatagcc
     5941 ctttttaatg atctggttat tgttaattcg ataaacgaga ttcattgttt aaaggtacaa
     6001 tgattgtttt ttatattttg ggaaattgga actacaatat gaaaattgtt tcagcatttg
     6061 tatttttaat ggcagcattg agttcgaagg cacaatgccc cgattctatg ccgctatggt
     6121 tgcttggtaa gtggcagatt cagaatgaat cgggtctatc ctttgaggaa tggacactga
     6181 gtaacgacac cctgatggta gggcgaacgt atagttttta cggaaacgat acacttgagc
     6241 tcgatcgtat gcgtattgtg tgcagtcagg ggtcagtggt tttttatatg aacgcatcgg
     6301 taaacaacaa taatatagtt gtaccttttt tccctgataa agataacaac agcaaagtat
     6361 gggctttcta taatccggac gctgctttcc cgcatacatt caggtacgtg tatgcatccg
     6421 ctgatgaatg ctttgtttct ttcgaaagca ataatcctct tgaagcttgt accgacttta
     6481 aaatgataag aatcagccgg taaatgcaag ctttgaattt cccatcgtat cagttttcaa
     6541 cccgcattgt tggagaaaga acagagattt tcgatcaatt ccggaaaaaa tatgttgtac
     6601 ttacgcccga agaatgggtt cgtcagcatt tgttaaggtt tttagttgag gaaagatcat
     6661 atccgccggg attgatttct gttgagaaaa gcattgatgt taacggttta caacgaaggt
     6721 acgatgcagt tgttttcgac cggagcggaa agcccctcat gattattgaa tgtaaggctc
     6781 cttcagtagc cgtaactcaa caagtgtttg atcaggtgta tgcgtatagt atgaatattc
     6841 cggcttcatt ttattttatt agcaatggaa ttatccatgc tgttgttaaa aaagctcagg
     6901 gaagtgcagc ggctttttta gatgatattc ctagctatat cgaaataaac tcttagtaat
     6961 cgcgaccaaa gaaatatgca aaagagaatc caaacgtaga gtttacagaa ctataggtct
     7021 cgtatttgtt catatcaatg aatccaaaag cataattggc tctgatctgg aattgtttgt
     7081 aatcgccacc aacagcaaaa ctcagacctg aatcgaatcg cttatactca tcatattcat
     7141 aaacagagta tgtctcattg tactcgggca tgtcgtggtt taccattcta gactggccaa
     7201 caaccgcaac gctcacataa ggccctatct gcccgaaaat actgaaatta cctataggaa
     7261 gtttgtaacc aacttcgata ggtatatcta tataactaaa tgtatttcgg atggtaaaga
     7321 cacccggagt tacaacgttt ttgtatccct tctgatagag cccaatggtt gtgccgacat
     7381 ataaaaaact ggtcaattca aattcagcag tggctccaac ctgaaaaccg ggtttacttg
     7441 catattccac agtgccatcg ttaaattttg taatcatgtt cgaaactgtg acacctgctc
     7501 tgggaccaac atgaaaacct tgagcaaaaa ttacaactgc tagaagcaac aagccgaagg
     7561 tagtaattaa ttttttcata atattgaatt tattaaggtt attacagtga aaaacaaatc
     7621 aatatgtaaa tataaaaaat attttaacga ctgtaaaatc attatctgtt tttttgtatt
     7681 tttagttatt aatttttaaa gaatatttta tgcgcttacc tgttgttatt atcctgcttt
     7741 tattgagtat ttctgcaatg ccgcagaaac tgatcagagg ctacacagtg ggttatgccc
     7801 atgggttcct tagccatggc gatgctattt ccattatcga tacaaccggc tatgttgatg
     7861 aatcgtatca tgcctatgcc gatgaagaga agctgaagta cgcgtcagcc agcagtttgt
     7921 attttggatt ccccattgaa tacggctact cgaggcatcg ctttactttg ctacctgctg
     7981 ttgatattaa cactgcccac tggtatctgc attttgaaga tccggaaagc atgcccttcg
     8041 gtttaagtgc taatgactcg ctctatttta aatcgtttat gattatgcct caggttggtt
     8101 taacgtataa atttcatttt tttgcgggag gaatccatct tgccgttggt gcaggagttg
     8161 atgttaaaat acctttgagc atcttaactc agaccagtct ggttgatagc gataaaaccg
     8221 agtatgaccc tacaatgttg ggatataaaa tccattatac accaaatacg attttcgata
     8281 ttgcaagcaa ccctggagtt cacctctcgc ctaaatttgt tttcgatatt tatttaagcc
     8341 gattcctggc tataaatctg atgtattacc gaaataaact taacagtggt gagcataatc
     8401 cattgctgtc tggatatttc ggaggaggcg ttacttatct gctcccattt ggaaaggaag
     8461 acgatatcag ggtgctgcaa cagtataaaa aggaataatt atgttaagcg aagtaatcaa
     8521 ttgtatccat caaaaatatg catatcaaca catcaaatag cagaactatg gcaaaatcaa
     8581 tgataattgc ttttttcctg acaataattg ttgtagggag caatgctcag aatgtaatac
     8641 cggtggttca aacggggcat tccggaaaaa tcaagtgcgt gaattgggat aataccggaa
     8701 ggtatattgc cagtgccgat atgaacaacg atgtagttat caacgatgta attgctggta
     8761 agcaattttt taaagtgaaa ctacccggta aaggaacggt tacaggtctg caattcgata
     8821 aaagcaaact gattattgcc aaccatgggc aaaattatgt tttcgatatg aacagcctta
     8881 cactcgagcc ttgcaatgaa tcgataaata ttgcccccga tgatggcggc tacaagctgc
     8941 ggggagcaac ggtgaagaaa ggcgcaaggt cgcttatcaa caaagatata tacgttcatt
     9001 tcactcaagt tgcttctcat cctaatggtg aactcgttat tgcaggagat gaggctggat
     9061 ggctgtattt ttgtaagcct tcgctgaata ttatcaagcg ggatcgtact cacaggctcg
     9121 atatcaccga tattgattat tccgacgacg ggaaatatat agccattgct tctgccgacc
     9181 gttctattag tatctggagc attgcagagc ttaagctgga gaagaggctt gttccgcgca
     9241 gttttaatat ttatggtatg gatgcacacc ccgatggtag ccgtctggct tttggcgacg
     9301 aactcggata tacttacctg ttagtatttg agaatgataa aattacttgt aagggtatta
     9361 atactcatag aagccgtgta aacgatgttt gctttacacc caaccctatg tgtgtcgcta
     9421 ccgctggtgg cgataacttt gcctcggttg tcgattttga aaataatata ccgttacagt
     9481 attttaaact tcagcctaat acatcgaagc tgaagcagac atttaatatt aagggaattc
     9541 aggcaaaagc taccagtacc gacgaagatg agattttctt tgatgaaaat gtatactcgg
     9601 tagctgttag ccctgatggg aaacaaatag cttactccgg cggtaaatgg ggaaatacca
     9661 acccaatgct aaaagtttct gcaatttcaa atctgaacct tgatgaaccc aaagagaaac
     9721 gtaagaagcg aaaaatggaa atcggcctta ctcaaaaaaa caacaaacat atttttcctc
     9781 aactgttctt tatcagcgaa aacgagttct atggtcttgg ggaaagcaat aacgaagccc
     9841 aaagatttaa actctcaatt accggttacg atgaaaaaga caatacaagt gttcgctcag
     9901 gcttcgacga taatgtcagg ccaaataata tgacagttat tgtatcgaaa tcgaaaaaat
     9961 cgatgaccga cgattatctt gttaaaaaag acctcaactc tgaagatgaa ttttcatgca
    10021 ggggatacac tatcaccaga acaagcagag gcagatcggt gaaattcgaa gggcacagag
    10081 gttatatcaa cgatgtggag attattcgcg ataaaaacta cctgattagt agtgccgaag
    10141 atgctgccat gataatttgg aacatcgaaa ccggcgagaa aataatgact gtttatgtgg
    10201 ttgaccaggg aaaacttgtt tttatttcac cggataatta ctacctcgct cagggagacg
    10261 cacttacggg aatgggcttt aattataacg gaagagtgct acctgccgaa cagttcgacc
    10321 tgaaatataa ccgacctgat attgtaatgg ataaactggg catctttaaa ccggatgtaa
    10381 ttgccttata ttataaagcg tatttgaaac gacttagtaa aacgggcttt aatgtagaaa
    10441 tgcttaaagc cgatatgaat ctgcccgaac tggtcatcga aaatgtaacg gatattcagt
    10501 ataagaccac acagcctgac ctgaaactga aactgaatat gagtgataag atgtatgaaa
    10561 tcgatagaat gaatgtgtgg gttaacgatg ttcctttgtt tggaaccaat ggtttgtcgc
    10621 ttattgataa gcacacaaag aataccggca ctgaggtaaa cattccatta tcggaaggaa
    10681 ataatgtgat acaggtttca tgcatgaact ctattggggt agagtcgctt aaggatgaat
    10741 tcgtaattga gagagaagta tcgaaaaagc gacaacccga tatatatctc gtatctattg
    10801 ctgtttccga ttttaaagag tcaaaatata atcttcgcta tacagttaac gatggaaagg
    10861 agtttattaa gcttttcgaa aaacgcggtg gcaattacgg acatgtatat gtcgattcat
    10921 tctataatgc ctcgtgcaca cgtgaaaatg tgttgaatgc aaagaatcgt ttgatgaaca
    10981 gtcatgtcga cgattacgtt attgtacaca ttgccgggca tggactcctt gacgacaatc
    11041 ttgattttta ttttgctacc tctgatatcg atttttcaaa cccatcggag agaggcttac
    11101 gatatgatga actggagggt ctgctcgatg gtataccggc acggaacaaa ctgtttctta
    11161 tggatgcctg ccatagcggt gaaattgaca agggcgatga ctttgccgtt gaacaggtta
    11221 ttggcgacaa tcagcgtggc gttacggttt ttactcctca gggcaatacg gtacccaaga
    11281 c
//
LOCUS       DAOOQP010000097        11164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7302_length_11164_cov_4.844991, whole genome shotgun sequence.
ACCESSION   DAOOQP010000097 DAOOQP010000000
VERSION     DAOOQP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7302_length_11164_cov_4.844991"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLK75_09725"
     CDS             <1..481
                     /locus_tag="PLK75_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY39565.1"
                     /translation="IGDTVSVLTEVDQENITYTWTPQIDISCFDCPSPYLYPKEDTRY
                     LLVVEDSAGCFRHNYYVDIYVVVAYSLEVPSAFTPLGPEVNSVVYAKGFGIKHFIEFR
                     IFNRWGEEVFSTDDITKGWDGYYKGELQNIDTYKYFVRAEMWNGEIFERKGDILLMR"
     gene            complement(556..996)
                     /locus_tag="PLK75_09730"
     CDS             complement(556..996)
                     /locus_tag="PLK75_09730"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY39566.1"
                     /translation="MGITRKATAVWNGTGKEGKGILSSTSGVLNETPYSFAARFVSED
                     GKAGTNPEELIAAAHAGCFAMALSFQLNGAGFTANELKAKAAVSMDNASGHFEFTGIH
                     LDLEAKIPGISQEKFMELAEVAKKNCPVSLALASVNITMDAKLK"
     gene            complement(1099..1734)
                     /locus_tag="PLK75_09735"
     CDS             complement(1099..1734)
                     /locus_tag="PLK75_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY39567.1"
                     /translation="MQIKSFVLNPFQVNAFVVFDESNEAVLIDAACMSKNEEDILCSF
                     IEGNKLVPVAIFSTHGHVDHICGNAFLKEKFAIPFFMNKADNFFIETALLHGSLFGLR
                     IKEPPLPDANPVQGDIFRFGNNSELHIIEVPGHSPGSIAIFSPKANFVITGDALFAGS
                     IGRTDLPKGNYSELISSVNEKLFTLPSNTQVLPGHGDFSTIEIEKAENPFF"
     gene            complement(1751..2620)
                     /locus_tag="PLK75_09740"
     CDS             complement(1751..2620)
                     /locus_tag="PLK75_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY39568.1"
                     /translation="MKKFIYILFRFLTAWFSITPFCIIYLFSDITFLVVYYVLGYRKK
                     VVYSNLKNSFPKKNDKEINKIALAYYRHLCDISVETIKGITLSQIVIKRRYHVVNPEI
                     LDKYFERGSNIICLASHYNNWEYGILALGPALKHQAVSIYLPFTNPYMEKYGVRERCK
                     YGMEMIPVNQTKEYFSRNHPKPFALILAADQSPSNPEKFIWVDFLQQDTACLHGAEAY
                     SKKLNAPLVYFDVQKTRRGFYRLKMEILVENPLETSNGEITAAYMKRLEKDIVSNPQF
                     WLWSHKRWKHKRN"
     gene            complement(2645..3346)
                     /locus_tag="PLK75_09745"
     CDS             complement(2645..3346)
                     /locus_tag="PLK75_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY39569.1"
                     /translation="MNKLLILWFWIVLPSCLFAWERKSDTTLYVGIQGGMSYCGWRNF
                     FDEYKHRLLPDYNIFAEFQPTREFSLRGGIGNTTKGISYRYTFYDVLHNPFDTSAFIN
                     LNYLTLPFTACYNLGRRFNPYLGVGFELNFLTKSRHFARLPETRNGIEVEPFDNEIKD
                     FYKNFDICLLAEAGFDYLIKPDFVVFIQARYHYSLLSIYKDSEAIPGGNLVKNNAIAI
                     NAGIKIGIPIKYTVY"
     gene            complement(3343..4737)
                     /gene="xseA"
                     /locus_tag="PLK75_09750"
     CDS             complement(3343..4737)
                     /gene="xseA"
                     /locus_tag="PLK75_09750"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOY39570.1"
                     /translation="MIETDRFSLSELNNLVGNTLKEHLDGFFWVVAEINNLNENRSGH
                     CYLELIEKSADGDHIIASAKAIMWAAKYRFIRAYFEQTTGESLKAGIKIMAKVSIEYH
                     SVYGLSFIISDIDAAYTLGEAALKRQSTIQSLMEAGVFDMNKSLPLPAVIKRIAVISS
                     RGAAGYEDFVNQLENNPYGYKFQLAPFEASMQGSDTEKTVVEAMNAIFYGKKNFDTVV
                     IIRGGGSKADLAAFDNYNIAYYITQFPIPILTGIGHERDESVADLVANTRLKTPTAVA
                     EFIIDRNQSYEQIVNSVYENIIERSQAIIRDHFSLIHYFSLDLIQRAKTISNKHKQKH
                     FILFQRLSNSSNRILMEKKLDMNTQTIALKSNSGYAISRSYDKIQTDSRELSRSWQHH
                     LLLMNEKINHFSKTIEIANPENILKHGFSITRIGGKALTSCKEIHAGQAISTQLFDGI
                     ITSIVENCDENTSK"
     gene            complement(4734..6401)
                     /locus_tag="PLK75_09755"
     CDS             complement(4734..6401)
                     /locus_tag="PLK75_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY39571.1"
                     /translation="MKKFIFSSILIFFGISAFPQIAQDKYWVSFTDKIGTPFTLEQPE
                     DYLSPRSIARREAQGIQIDSLDLPVNPAYVIAVRDMGVEILNVSKWLNGVTIYTTNPN
                     IINDISQLNFVNSVSKSKKKKGKNKNSLEPMPYFDSNFNLREAESSDYFDYGLCIDQI
                     YIHNGQVLHNEGFRGEGMLIAITDAGFQGYDLPAFDSIRNNNQVVGTKNFLNKTESVF
                     NYSEHGMNVLSILAGNIPGQLIGSAPYANYLLLVTEDAASEQIIEEINWSCGAEYADS
                     AGADLISVSLGYFDYDFPNWSHSVAELDGNTAWVSRAANVAVGRGMIVVVAAGNNGDE
                     EIPYISMPGDSYEVVTVGAVGADSIRADFSSIGPTSDGRIKPDVCAIGYNTYCQQSFG
                     GVWPGSGTSFATPVMSGLIACLWQAHPEMNRYEVISAVINAGNMFSNPDNFMGAGIPN
                     FQIAHNNLSNVKYLNTGSDCKLFIAPNPFSDSISLSIRNLSSTSFTILITSIDGKALY
                     ADEFTASNTQEANLVIHDLSLQGNGMYLLHLDADNIHITRKIVKTGK"
     gene            complement(6413..7111)
                     /locus_tag="PLK75_09760"
     CDS             complement(6413..7111)
                     /locus_tag="PLK75_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39572.1"
                     /translation="MKQWVFILISFSLFAASCDNTPEAEPAGLYGLQYFPLDTSKFIL
                     YDVVEITIDKPVNVYDTLHYQLMERYSEPYTDNSGNLAYKMERWVRDDDLSNFTLSDV
                     WVAQVSDQSAQTVEENVRFVKIRFPARPNRSWNGNVFNIYESQEYYITNIDKAAEING
                     FSFDSTLTVVQQLDSSLIHKNDCIEIYAKNIGLVFKQKININSQEVIPNVDIDDRITT
                     GTKYYQKIIGFGIN"
     gene            complement(7121..8203)
                     /gene="mnmA"
                     /locus_tag="PLK75_09765"
     CDS             complement(7121..8203)
                     /gene="mnmA"
                     /locus_tag="PLK75_09765"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOY39573.1"
                     /translation="MTSGNKKVLIAMSGGLDSSVAALLLQSQGYELTGVTFIPYESIS
                     QACLEKQTGCCNADTIFEAGKLAAKLGFEHKIIDVRDTFRETVVKNFTSEYMHGRTPN
                     PCVLCNKLIKWGVLSLEAEKNGCSFIATGHYARIGNSGENYFLQKGTDLSKDQSYFLW
                     MLTSENLCRTLFPLGNMTKNEVREIARKNGFTKIADRRESQEVCFIPDNNYRRFLAEE
                     LSSLPGEGDIRDINGKIIGKHKGFPFYTIGQRKGLQVATGQPVYVVSIDSNTNTIILG
                     SKNHLLAKKTQLENAVFNTKLIIGKTYTVKIRYRTPGVKANLTIEDGKYIINFMEEAY
                     AITPGQSAVVYDDIDVIGGGIIAKID"
     gene            complement(8206..9021)
                     /locus_tag="PLK75_09770"
     CDS             complement(8206..9021)
                     /locus_tag="PLK75_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOY39574.1"
                     /translation="MTKSLLVIIASLFMPMLSYSQVGTYNNDTLINYIDINMKKQGKW
                     VKKYDDGQVRYKGFFINDEPVGTFIYYHTNGKIKSILDYTYPDAVKAELYYPDATIAA
                     AGKYNKSRERIGLWKMYYESGDLFAEINYKANVKHGTSTTYYPSGVILMECTFVDGLL
                     QGSYVKYFDTGKPYEIGSYKNNQRHGECSVYDLTNQLTEKGPYFEGMRHGVWYFFAEG
                     NIVDTVTFTYDMPDNFDEISKETQEKIEWARQNQDKFKQPEDYFDRPEDFFKP"
     gene            9290..10201
                     /locus_tag="PLK75_09775"
     CDS             9290..10201
                     /locus_tag="PLK75_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY39575.1"
                     /translation="MALRTVSLMICIFSASVLFSQSLVLDTIEVSDSTIVLYRDFTWK
                     YVSAIDNKEVLLLETSDSINLHDAIYEPDSSEVFGTYWDSVELFAYGGMNYSVCNDTL
                     AIPLLQDPFTIPVVGRIYSEFGYRGSRFHNGVDIDLKTGDPVRASFGGKVRKAYYNPG
                     GYGYLIVIRHYNGLETYYAHLSKLLVAEGQWVNSGDTIALGGATGRAYSPHLHWEIRY
                     KDNSFNPRLIADFEHSVSACDTLILKPTDFKHVKDLSEAKYHYIVQGDTLWGLSRRYG
                     VSVSQLCRLNNISETTTLRLGTSLRVR"
     gene            10297..>11164
                     /locus_tag="PLK75_09780"
     CDS             10297..>11164
                     /locus_tag="PLK75_09780"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOY39576.1"
                     /translation="MKKNKKKRYSLFLEAFRVNLLVAFTAIFIWQIIQAFDTLNPIDQ
                     MFEDFDYTDLYFSTFISKKMPPDTNVIIVNIGGRQFNRANIANQINVLQKYNPRVIGI
                     DAVFAERKGMEDFMLKQALNQKNNIVMGVFGDYDKDLNLVGYTRSNPFFGEFPFGHLE
                     FAVFSKTSRDFEKFITAYDTVVNSTCKELLLKYSQIYSEIEWARNSATDTATELVPID
                     TTIIRQKEMVLDSLRTAFFTQFVMRKPVKGFKEVRINAFTTEIIKMYDQKIYEKYLKR
                     TNTKEVINYHGGE"
BASE COUNT         3189 a         2227 c         2139 g         3609 t
ORIGIN      
        1 aatcggtgat accgtgagcg tactaacaga agtcgatcag gagaatataa cctatacatg
       61 gactcctcag attgatatat cctgtttcga ttgccccagc ccgtatttat atcccaaaga
      121 agacacccgt tatcttctgg ttgttgagga ttctgcaggt tgcttccgtc ataactatta
      181 tgtagatatt tatgtagttg ttgcctactc ccttgaagtg ccttctgctt ttacgcctct
      241 tggtccagaa gtgaatagtg ttgtttatgc aaaagggttt ggtattaaac atttcattga
      301 attccgcatt tttaaccgtt ggggagagga agttttttct accgatgata taacaaaagg
      361 ctgggatgga tattataaag gcgaacttca gaatatcgat acatacaaat atttcgttcg
      421 tgccgaaatg tggaatggag agattttcga aagaaaagga gatattttat tgatgcgata
      481 attgattgat gtctgtttct gaaatagaaa agccggctaa aaccggcttt ttcaataaaa
      541 taatatctgc ctgatttact ttaattttgc atccatggtt atattaaccg aggctaaggc
      601 cagagagacc ggacaattct tttttgcaac ttcggccaac tccatgaatt tctcttgtga
      661 aatgccggga atttttgctt ctaaatccag atgtatcccg gtgaactcaa aatgtccgct
      721 tgcattatcc atacttactg ctgcttttgc tttcagttca ttagcggtaa atccggcccc
      781 gttgagctgg aaactgagtg ccatggcaaa acatcctgca tgggctgcag caatcagttc
      841 ctccggattg gtacctgctt tcccgtcttc gctaacaaac cttgctgcga aagaataggg
      901 ggtctcgttg agaacgccac tggttgatga caaaattcct ttaccttctt ttcctgtgcc
      961 gttccatacg gctgttgctt tacgtgtgat tcccatagtt ggtttgttta atgagttatt
     1021 gatattaata tttgaattat aacctgtatc ttaacaaatt gttcatacat tgatagatgt
     1081 tttcaattat tttgaaggtt aaaaaaatgg gttctcggct ttttcaattt caatggtgga
     1141 aaaatcgcca tggccaggta acacctgcgt gttggagggc aacgtaaaaa gtttctcatt
     1201 cacacttgaa ataagctcgc tataatttcc ttttggcaaa tcggttcgtc caatgcttcc
     1261 tgcaaacagc gcatcacctg ttattacaaa attggccttc ggagagaata tcgcaatgct
     1321 ccccggtgaa tgccctggaa cctctataat atgtaattct gaattgttcc caaacctgaa
     1381 aatgtctccc tgaacagggt ttgcatcggg taatggcggt tctttgattc gtaacccgaa
     1441 taaactcccg tgcaatagtg cagtctcgat gaagaaatta tctgctttat tcataaaaaa
     1501 cggtatcgca aatttttctt tgagaaaagc attaccgcaa atatgatcaa catgaccatg
     1561 tgtgctgaaa atagcaacgg gtacgagctt gtttccctcg atgaagctgc ataaaatgtc
     1621 ttcttcattc ttgctcatgc atgcagcatc aataagtact gcctcgttac tttcgtcgaa
     1681 tactacaaat gcatttacct gaaatgggtt taaaacaaac gatttaatct gcatactttg
     1741 atttttgcat ttaattccgt ttatgtttcc accttttgtg actccataac cagaactgcg
     1801 gattacttac aatatctttt tcgagccttt tcatgtaagc cgcagtaatt tctccattgg
     1861 aggtttcaag cgggttctca actagaattt ccatttttaa ccggtaaaaa ccacggcgtg
     1921 ttttttgcac atcaaagtaa accagaggtg catttaattt ctttgaatag gcttcagctc
     1981 catgcaaaca ggcagtatcc tgttgcaaaa aatcgaccca aataaatttt tcgggatttg
     2041 atgggctttg atctgcggca agaataagtg cgaacggttt gggatgatta cgagaaaaat
     2101 attctttggt ttgattaaca ggaatcattt ccataccata tttgcatctt tctctaacac
     2161 cgtatttttc catgtacgga ttggtaaatg gaagatatat agaaacagcc tgatgcttca
     2221 atgccggacc aagggcaagg attccatatt cccagttgtt gtaatggctc gcaagacaaa
     2281 ttatatttga acctctttcg aaatacttat caagtatttc tgggttcaca acatggtagc
     2341 gcctttttat aacaatttgg cttagggtaa tgccttttat cgtttctacg ctgatgtcgc
     2401 ataaatgtcg gtaatacgcc agagctattt tattgatttc tttatcgttt tttttgggga
     2461 atgaattttt aagattgcta tatactactt ttttacggta acccagtaca taataaacaa
     2521 ccaaaaaggt gatatcggaa aacagataaa taatgcaaaa tggtgtaata ctaaaccatg
     2581 ccgtaagaaa tcgaaataaa atatatataa attttttcat cgggcaaatg taataaaaac
     2641 ggtgttaata aaccgtgtac ttaatgggta tgcctatttt aatacctgcg tttattgcaa
     2701 tggcattgtt ttttactagg tttcctccgg gtatcgcctc gctatcctta tatatgctta
     2761 acagcgaata atgatatcgt gcctgaataa atacaacgaa atccggtttt atgagataat
     2821 caaaacctgc ttctgccaac aaacaaatat cgaaattttt atagaagtct tttatttcat
     2881 tatcgaatgg ctctacctca attccgttgc gtgtttcagg caatcttgca aaatgtcgtg
     2941 attttgttag aaaatttaac tcgaacccaa ccccaagata tgggttaaac ctcctcccaa
     3001 ggttgtagca agctgtaaat ggtagggtca agtagttcag atttataaat gcactggtat
     3061 caaacggatt gtggagtaca tcataaaaag tataccggta acttattcct ttggttgtat
     3121 tgccaatgcc tcctctaagc gaaaattcgc gggtgggctg aaactcagcg aaaatattgt
     3181 aatcgggtaa tagcctgtgc ttgtattcat cgaaaaaatt tcgccatccg caatagctca
     3241 tgccaccctg aatacccaca taaagtgttg tgtctgattt gcgttcccac gcgaaaaggc
     3301 atgaaggtaa cacaatccaa aaccatagta taagtaattt attcatttac tggtgttttc
     3361 gtcgcagttt tcaacaatac tggttattat tccgtcgaat aattgggtgc ttattgcctg
     3421 ccctgcatga atctctttac acgacgtcaa tgccttacca ccgatacgtg ttatcgagaa
     3481 cccatgcttt aggatattct ccggatttgc aatttctatc gtttttgaaa aatggttgat
     3541 tttttcattc ataagaagca ggtgatgttg ccatgacctg ctgagttcgc ggctgtctgt
     3601 ttgtatttta tcgtatgacc ggctgattgc atacccggaa ttcgatttca atgcaattgt
     3661 ttgagtgttc atatccagtt ttttctccat taatattcta ttggaactat tgctcaaacg
     3721 ctgaaataga atgaagtgtt tttgcttgtg tttgttgcta atagtttttg ccctttgaat
     3781 aagatccaga gaaaagtagt gaatcaaact gaaatggtct ctgattatag cctgacttct
     3841 ttcaataatg ttctcataaa cagaattgac aatttgctca taactttgat ttcggtcaat
     3901 tatgaattct gcaacagctg taggagtttt tagacgggta tttgcaacca agtcggcaac
     3961 agactcatcg cgttcgtgac caattcccgt aagaatcggt atcgggaatt gggtaatata
     4021 ataggctatg ttgtaattat cgaatgcagc taagtctgct tttgaacctc cgccacggat
     4081 gattaccacg gtgtcgaaat ttttcttgcc ataaaaaatt gcattcatgg cttcaacaac
     4141 cgttttttca gtatcgcttc cctgcatgct tgcttcgaaa ggagccaatt gaaatttgta
     4201 gccatacgga ttgttttcaa gctggttgac aaaatcttcg taccctgcag ctcccctcga
     4261 tgatataact gctatacgtt ttattacggc aggcagagga agcgatttat tcatatcgaa
     4321 tactccggct tccataagac tctgaatagt ggactgtcgc tttagtgctg cttctccaag
     4381 agtataggca gcatcaatat cgctaataat aaagcttaag ccatacactg aatgatattc
     4441 aatgctcact tttgccatga ttttaattcc ggcttttaga ctttctccgg tggtctgttc
     4501 gaaataagcc cttataaagc gatattttgc agcccacatt attgcttttg ccgaggcaat
     4561 gatatggtcg ccatcagccg atttttcgat tagctccaaa tagcagtgac ctgaccggtt
     4621 ttcgtttagg ttgtttattt ctgcaacaac ccagaaaaat ccgtcaaggt gttctttcag
     4681 agtattacct acaagattgt ttaattccga taatgaaaaa cggtcagttt caatcatttt
     4741 ccggtcttga caatttttcg tgttatatgg atattatcag catcaagatg aagtagatac
     4801 atgccatttc cctgaagcga taaatcatgt attacaaggt ttgcctcctg agtattagat
     4861 gcagtgaatt catcagcgta caacgccttg ccatcgatgc ttgttatcaa aattgtaaat
     4921 gacgttgagc tcaggtttct tatacttagg ctgattgagt cgctgaacgg gttaggagct
     4981 ataaaaagct tacaatcgct tccggtgttt aagtatttta cattgctcag attattgtga
     5041 gctatttgaa aatttggaat tccggcaccc ataaaattat cggggttcga aaacatgtta
     5101 cctgcattga taacggcgct gataacctcg taacggttca tttccggatg agcttgccac
     5161 aggcaggcaa ttaatcctga catcacgggt gttgcaaatg atgttcctga gcccggccac
     5221 accccgccaa acgattgctg acaatatgta ttgtaaccta tagcacacac gtcaggtttt
     5281 attctgccat cactggttgg gccaatactg ctgaaatcag cccgaatact gtcggcacca
     5341 acagcgccaa cggtaactac ttcgtacgaa tcgcccggca tgcttatata tggtatttct
     5401 tcgtccccat tatttccggc tgctacaacc acaatcattc ccctgccaac agcaacattg
     5461 gcagcacggc ttacccaggc agtattgcca tcaagttctg ccactgagtg cgaccagttg
     5521 gggaaatcat aatcaaaata gcccaatgat acactaataa ggtcagcacc agcactgtcg
     5581 gcatattctg caccacacga ccagttgatt tcctcaatta tctgctcgct ggcagcatct
     5641 tcggttacca ataacaggta gttggcataa ggtgcagacc cgataagctg tccgggaata
     5701 tttcccgcaa gaattgagag aacattcata ccatgttcgc tgtaattaaa aacactttcg
     5761 gttttattta gaaaattttt tgtaccgaca acctgattat tgtttctaat gctgtcgaaa
     5821 gcaggtaaat catacccttg aaaacctgcg tcagttattg ctataagcat tccttctcca
     5881 cgaaaacctt cgttatgaag aacctgtccg ttgtgtatat atatctgatc aatgcaaagt
     5941 ccataatcga aataatcgct tgattcagct tctcttaaat taaaattact gtcgaaatag
     6001 ggcatgggtt caagcgagtt tttattcttt ccctttttct ttttcgactt cgacactgaa
     6061 tttacaaaat ttagctgcga tatatcgttt attatgtttg ggttggttgt atatattgta
     6121 actccgttta accatttcga aacattcaga atctcaactc ccatatccct tacagcaata
     6181 acataagcag ggtttacagg taaatcgagt gagtcgattt gaatgccctg tgcttcgcgc
     6241 cttgcaatgg acctggggct caaataatct tcgggttgtt ccagggtgaa aggagttcct
     6301 attttatcgg taaacgaaac ccagtactta tcctgtgcaa tctgaggaaa tgcacttatt
     6361 ccgaaaaaga ttaaaatgct gctgaaaatg aattttttca tttttttctg tattaattga
     6421 taccaaatcc gattattttc tgatagtatt ttgtccccgt tgtgattctg tcgtcgatat
     6481 caacattggg aattacctcc tgcgaattga tgtttatttt ttgcttgaaa accagcccaa
     6541 tatttttagc atatatttct atgcagtcgt ttttatgaat cagcgaggaa tccagttgct
     6601 gaaccactgt taaggttgaa tcaaacgaaa atccattgat ttctgcagct ttatcgatgt
     6661 ttgtaatata gtattcctgc gattcgtata tattgaatac attgccgttc caacttctgt
     6721 tgggtcgggc agggaacctg atttttacga aacgaacatt ttcttcaacg gtttgtgccg
     6781 actgatcgga aacctgagcc acccaaacat ccgataaagt aaaattgctc aagtcatcat
     6841 ctctgaccca cctttccatt ttatacgcaa gatttccgga attatcggtg taaggttctg
     6901 agtatctttc cattaactgg tagtgtaagg tgtcgtaaac atttacaggt ttatcaattg
     6961 taatctccac tacatcatat aaaataaact tcgaagtgtc gagaggaaaa tactgcaaac
     7021 cgtaaagtcc ggcaggctca gcttcaggtg tattgtcgca tgatgcagcg aacaacgaaa
     7081 acgaaatcaa tatgaaaacc cattgtttca ttaagcaaat ttagtcaatt ttcgcaataa
     7141 tccctccgcc aattacatcg atatcatcat atacaacagc cgattgtccg ggggttatgg
     7201 catatgcctc ttccatgaag ttaattatat actttccatc ttctatcgtc agatttgctt
     7261 taacccccgg tgtacggtaa cgtattttta cagtataggt tttccctatg ataagttttg
     7321 tgttaaaaac cgcattttcg agctgagttt tttttgcgag caggtgattt ttcgaaccga
     7381 gaattattgt attcgtattg ctatcaatag aaaccacata aacgggctgc ccggttgcaa
     7441 cctgtaaacc ttttcgctga ccaatggtat aaaacggaaa acctttgtgt ttgccaataa
     7501 ttttaccatt aatatctctg atatcacctt cccctggtaa tgagcttagt tcttctgcaa
     7561 ggaatctgcg atagttgttg tcaggaataa aacacacttc ctggctttct cttctgtcgg
     7621 caatttttgt aaacccattt ttacgggcaa tttcgcgtac ttcatttttt gtcatattgc
     7681 caagcgggaa aagggttctg cataagttct cgctggtaag catccacaaa aaataagatt
     7741 ggtctttcga aaggtctgtt cctttttgca ggaaatagtt ttcgccacta ttaccaattc
     7801 tggcataatg ccctgttgca ataaaactgc atccgttctt ttcagcctcc agcgacaata
     7861 ctccccattt tataagcttg ttacataaaa cacatggatt tggagttctt ccgtgcatgt
     7921 attccgaagt gaagttttta acaactgttt ctctgaaagt atctcttaca tcaataattt
     7981 tatgctcaaa acccagcttt gctgcaagtt tacctgcttc gaaaatagta tcggcattac
     8041 aacatccggt ttgtttttct aaacaagcct gagaaattga ttcgtacggg ataaaagtta
     8101 cacctgttag ttcatagcct tgtgattgaa gcaataatgc agcaactgag ctgtcgagcc
     8161 cgccgctcat tgcaatcagg acttttttat ttccgctggt catgatcagg gtttgaaaaa
     8221 gtcttccgga cggtcgaaat aatcttccgg ttgcttgaat ttatcctgat tttgcctggc
     8281 ccattcaatt ttttcctgtg tttcttttga tatctcatcg aaattatcag gcatatcata
     8341 agtaaatgta acagtgtcaa caatattacc ttcagcgaaa aaataccata ctccatgacg
     8401 catcccttcg aaataggggc ctttttcagt aagctgattg gtaagatcat ataccgagca
     8461 ttccccgtgt cgctgattgt ttttataact gcctatttcg taaggttttc cggtatcgaa
     8521 atatttaaca tatgagccct gaagtaaacc atcaacaaaa gtacattcca tcagtattac
     8581 acccgatggg taatatgttg ttgatgttcc atgttttaca ttggctttgt aattaatttc
     8641 agcaaataaa tcgccgctct cgtaatacat tttccacaac ccgatacgtt cacgcgattt
     8701 gttgtatttt ccggcggcag caatggtagc atcagggtaa taaagttcgg cttttacagc
     8761 atccggatag gtgtagtcaa ggatagattt tatttttcca ttcgtatggt aataaataaa
     8821 agtgcctaca ggctcatcgt tgatgaaaaa gcctttgtaa cgaacctgac catcatcgta
     8881 tttttttacc catttcccct gcttcttcat gttgatatca atgtagttta tcaaggtgtc
     8941 gttgttgtac gtccccacct gtgaatacga aagcattggc ataaacagtg aagcaataat
     9001 tacaagtaaa ctctttgtca tggttcttat catttgaatt ttcgtttagc aaatttaatg
     9061 cctctatgca agtgtaaaaa caaaaacgca ggaaaataat atttatttta taaacatttg
     9121 cctgcttaaa ttttgacagg cctattaaga ggctcagaca tacattgcaa agaaatctgt
     9181 tataatcctt cccaagttta ataaatgaca tacgttttat ttgccacgac aacataaccc
     9241 ctgtttaatt tttattgaca tatttgcatc aggataatta atataaggta tggctttaag
     9301 aactgtaagt ctgatgattt gtattttttc agcatcagtg ctgttttcac agtcattggt
     9361 gttggacacg attgaagtaa gtgactcaac aattgtattg tatcgggatt ttacatggaa
     9421 atatgtttcc gctattgata ataaagaggt acttctgctt gaaactagtg attcaatcaa
     9481 cttacatgat gccatatatg agcccgacag ctccgaagta tttggcacat attgggattc
     9541 ggttgagctt tttgcatatg gcggcatgaa ttactctgtt tgcaacgata ctttggctat
     9601 acctttgtta caggacccat ttactattcc tgtcgtgggg cgtatctaca gtgaattcgg
     9661 ctatcgcggt agtcgttttc ataacggggt tgatattgat ctgaaaaccg gtgacccggt
     9721 aagagcttca ttcggtggaa aagttcgcaa ggcgtattac aaccccggtg gctacggata
     9781 tttaatagta ataagacact acaacggtct tgaaacctac tatgcccatc tatcgaaact
     9841 tctggttgca gaagggcaat gggttaattc gggagacaca attgcattgg gtggagccac
     9901 cggaagagca tattcacctc acttgcactg ggagatccgg tataaagaca attcgtttaa
     9961 cccacgtctt attgccgatt ttgagcattc cgtgtctgca tgcgatacac ttattcttaa
    10021 gccaaccgat tttaaacatg taaaggatct ttctgaagca aaatatcatt acattgtgca
    10081 gggcgacacg ctttgggggc tttcaagaag gtacggcgtt tcggtgtcgc agctttgccg
    10141 actgaacaac ataagcgaaa ctaccacact gcgactgggc acttcactcc gggtgagata
    10201 gcattgtttt tcggtactac ttatattttt attcctgtgt gagaaatctt gtaaatcgta
    10261 gtgttttttt tcttaatttt atgcaaatgt tttcccatga aaaagaataa gaaaaaaaga
    10321 tactcacttt tcttagaagc attccgcgtt aatctcctgg tagcttttac agcaatattt
    10381 atctggcaga tcattcaagc gttcgatacc ttgaacccca ttgaccagat gtttgaagac
    10441 ttcgattata ccgatttgta tttttcgaca tttatatcaa aaaaaatgcc tcccgataca
    10501 aatgtaatta ttgtaaatat tggtggcagg caattcaata gggcaaatat tgccaaccag
    10561 attaatgttc tgcaaaaata taatccacgc gtaataggga ttgatgctgt ttttgctgaa
    10621 cggaagggga tggaggattt catgcttaaa caagcattga atcagaaaaa caatattgta
    10681 atgggtgttt ttggagacta cgacaaagat ttgaatttag taggatacac ccgaagtaat
    10741 ccgtttttcg gagagttccc attcggacat ctggaatttg cagttttttc aaaaacaagc
    10801 cgcgattttg aaaaattcat tactgcctac gacacggttg taaacagtac atgcaaagag
    10861 cttctgctaa aatattccca gatatatagc gaaattgagt gggccaggaa tagtgcaact
    10921 gacactgcaa cagagttagt acccattgat acaaccatta ttagacaaaa agaaatggtg
    10981 ctcgattcat tgcgaacggc attctttact cagtttgtta tgagaaaacc ggtgaaaggc
    11041 tttaaggaag tcagaatcaa tgcttttacc accgagatta taaaaatgta tgaccagaaa
    11101 atctacgaaa aatatctgaa gcgtactaat acaaaagagg ttattaatta tcacggtggc
    11161 gaaa
//
LOCUS       DAOOQP010000098        11132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7324_length_11132_cov_4.802925, whole genome shotgun sequence.
ACCESSION   DAOOQP010000098 DAOOQP010000000
VERSION     DAOOQP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7324_length_11132_cov_4.802925"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLK75_09785"
     CDS             complement(<1..228)
                     /locus_tag="PLK75_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39577.1"
                     /translation="MNKVLILISISIIAVNAWGQSTKDTIIEDHKLTNIPVISLSDSD
                     LQNDAQSQDISGLLQSSSDVFVSVAGYTFGQA"
     gene            complement(327..2228)
                     /locus_tag="PLK75_09790"
     CDS             complement(327..2228)
                     /locus_tag="PLK75_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOY39578.1"
                     /translation="MSDAIKHECGIAVIRLLKPLEYYQEKYGTWQWGLNKLYLLMEKQ
                     HNRGQDGAGAVCIKTDMDPGNKYINRFRSVRINPIQHIFEQISENLTQLALNGNGTIT
                     DCNEAKRNFDFAGELYMGHLRYGTFGKNDIENAHPVIRQNNWRSRTLVMAGNFNLTNV
                     DELFKYLVDFGQNPRDYSDTVTALEMVGLHLDEENQRLFRDYKNRGIAHKDISPLIEK
                     NIDLVKILASASQNWDGGYVMAGMLGHGDTFIIRDPWGIRPAFYFKNDEIAVAASERP
                     VIQTVFNVPFNEIKEVEPGHALIIKKNGDTLHSLIRVPRVKTPCSFERIYFSRGTDTD
                     IYNERKKLGELLVPKILESINHDFENTVFSFIPNTAETAFYGMLKGLENYLLQRNKKI
                     ISENREHLNDEMLDKIFSLRPRVEKIAVKDMKLRTFISSDKDRNNLVHHVYDITYGTI
                     VPHTDTLVIIDDSIVRGTTLRESILRILDRLQPKKIFIVSSAPQIRYPDCYGIDMAKL
                     GDFVAFQAAIVLLKENGMEHVIENVYQKCKSQQYLPKEEMINYVGHIYNTFTEEQISH
                     KISELIRDEAIHAEVGIIFQRVEDLHKACWNNHGDWYFTGEYPTPGGNKVVNTSFINY
                     IEGRNERAY"
     gene            2483..3937
                     /locus_tag="PLK75_09795"
     CDS             2483..3937
                     /locus_tag="PLK75_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39579.1"
                     /translation="MKRALLFEFIMVLFVGIQAQTINNSSTKVNIPDGTYVIFSNLIN
                     SGSGAEFNYNNDLTISGNWTNSNAAVFTSGPNSSVVFNGSSQQTITSGGSQFRNVVIN
                     NTSTTDYAIILGDDMSISAQLTLTDGVINGGTYQVKILNEATSSAGSAISYVDGIVAK
                     TGTVAFTFPVGDGLVWAPVGIAAPSTSSTISAQYTFSEGPLNWSASYMCDESQMQYTS
                     GVEHWDMATTSATPAVTLYWKNGTRSGITDLTDLAVAHFNGTCWEYMEGTTTGNTSDG
                     SITSTLPFSSYSPVSFGSKNRINPLPIELLSFSSNCTGYGVSLEWVSASESNSYGYYI
                     EKSLDAVNFKEIGFVDAAGNSNQVLNYSFTDENVEKQAKYYRLRMVDVDGQFKYSSTI
                     VAFCYEAGAPVIMVYPNPFKDDLNIIGENFRDENTIIYVYDMLGKIIAEYKVENLNGS
                     FSQIINIHDLSPAMYTVKIISGSFNRIVKVTKEQ"
     gene            4003..6801
                     /locus_tag="PLK75_09800"
     CDS             4003..6801
                     /locus_tag="PLK75_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39580.1"
                     /translation="MKTKIAFLFFVVIMLYVTTINLRAQGVAINLDGTAADASSMLDV
                     KATNRGLLIPRVALTQTTSASPVSNPTASLMIYNTASTGDVTPGYYYWSGSAWVRFGS
                     AVHTHATLTQGTGMNTFSYNGSSSATVALANTTVTPGTYGSASQVGTFTVDAQGRLTS
                     ASNVNMTMPTSTGALNMVYPVYIRGTGLNNNANQVLIVGSTTIYNTGARGLRLTVLSK
                     TDFSVVSDATYDTYGSTTASDNLAIALNGLNNSQIGVLTSWDAWEGAVTTNLENAFKR
                     LGLYKAYATVNSGSRRTYSAIFEAASSGETTDKAVEVSTDNTANQPYAEIRGWLINGS
                     FVASGSQKNALTTPQGTALGLIVDPSGYVGIGTDNPGSRLDVSGGNVRTTNQFVSTIA
                     TGNAPIVVSSTTLNTNLNSDMLDGLHLNSTTTNNQVNAVVRTDANGYANFGWINTISG
                     DNGTTAISRIYASNDAYIRYYTPANFITAMGLHTGSGSANYLARWTGSTTLGTGVTYD
                     NGTNVGIGNYSPAYKLHVGSGNGDGIIIGNYNDRLGWDGSGAQPELAIRFAGYRDVVS
                     NFTGAKISAIRTNICCSALSQGTELAFYTQESTAIGSGDSNLSEKMRIGSGGNVGIGT
                     SYPGEKLVVSTSSTNIAVYGYNGRSTSSANNIGVEGVATGTTGSSYRNIGVYGYAADN
                     TSAEDYGVLGSCANTTDDWAGFFAGDVYASDYYDIAEMYSDAGEIPDGALVSLIQPIN
                     DPADAKVQISQNAYDPLLIGVKSENPALYIGPTESGLEVIPKYRELLKTREEILHKIY
                     IEQVGEQAVDLHQQLETVDNEISQLLIKMNPKGKGDVGSPIALVGRVNVLACTENGPI
                     KPGDPLTSSSKPGYAMKATKPCQIVGFSMSELSAGEGKIVLNVNHSFYNPFSETTESL
                     WQYIYSLEKRIEGLENKQ"
     gene            6952..10914
                     /locus_tag="PLK75_09805"
     CDS             6952..10914
                     /locus_tag="PLK75_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39581.1"
                     /translation="MKTQKVLFLLACIIVSFTNSVVFAQGVAINLDGTAADASAMLDV
                     KATNRGLLIPRVALTQTTSASPVSNPVASLMVYNTATANDVTPGYYYWSGSAWVRFGS
                     AVHTHATLTRGAGLTGNNYDGSTTTTWAVDFGNTTVAGNGPKSQGNFGQFQPHGTYTN
                     FNTDPAYWGWNYVQGNTNAPNTNSSQWYREVISLGDAYSARGTGGYSLELAFPRFSAS
                     TAGIWMRTVENGTIGAWTRIDANGINYSNLAGIPVRNAWSGVHRGFVAEQLSWKNYGN
                     NHTIFDASQGTSPDGTAVNNTNSQVAWTASYPTLMGWNGSNTYGVRVDVARYAESAGS
                     APGDNLGNHTATTTLNMNNNQITNFSYMQPYGVGGDSGQGNNAYGIFQEGGSWSSPYP
                     DLRIAFHTGINLGANASYGGIGFYTDYDMGTLVMSINDAVTGAGANNVYMAGGLKAGS
                     AYNINIPNSCGGNWATNCDLAIWTADHTGGCGDEAYIAHYIRTGEATTLEIGNKNDAD
                     DHIALMPSGNVGIGTTAPDQKLVVNGGNVDVTAGGGGLRLGSNNWSDGGSTYGHIVTD
                     NGSYDALMIVGNNTAGDGHRNVRIWDHLNVEGTLTRGGNTVWDAANDGSGSSLDADLL
                     DGNHASAFAASSHTHPWNQVTSKPSTWLDGATLIEDNADFNNSRPSGFYQYHNAANAP
                     TGGTWYNMINVRHSNTGNDHGFQIAACYYDENIWTRTYQGGTGHNDGSFTPWRALIHT
                     GNLSSNAILNQYSGAQSANMWISGETRTGGWFRSSSGSTGWYSEPYGSGIYATTSGEV
                     ATYNGSYFRITGSANGSGNLRFDAANPYIYASSYFLAPGGAYFNSGTVYTEAQYQCRG
                     GIHNDAAGDLTIAGGTNGTTYFSGPISGMSGTIPSNYMVRLTPNLHLNAGTGYAVIAN
                     WDNGTTGTSQALRVGNGAGSDAFYVRADSYTWAGTTSTSLSNVIYASRPSGNYGGGRA
                     TIYGYRYGGSSGSDGSSYSVYGTDAGVEGYTFYGNLYSFGVAGHTYGDYNRTGGTMGA
                     DNNGSYWGSLGYKNSGSSWYGLYYTSAGSGGGYMNNNGIISGIGSGGCGGVMGGWSRG
                     EVLGFTTAGELYASYNMGNEYTSGVSADIVTTSTERIPVYSVTSNDVKVYADGTAQLV
                     NGKCFVAFDESFVNVISKEGKPTITVTPNGECNGLFTVDISSRGFTVKELNNGSSTVE
                     FSWIAIGKRIDAEKAGDLPQAIKDKNFDNNLKGVMFNENDKDNSATPIWWDGNNLRFD
                     KMPETPVNKPKDDPNLPAVNNSDDVNRLIQQNKQSQPENKEWRPTPEEPIRIQGNEKK
                     "
BASE COUNT         3262 a         2161 c         2516 g         3193 t
ORIGIN      
        1 tgcctgcccg aaagtgtagc ctgcaaccga aacaaaaaca tcgcttgatg actgcaataa
       61 cccacttata tcctgcgatt gtgcatcatt ttgcaaatca gagtccgata gcgaaatcac
      121 cggaatattg gtaagtttgt gatcttctat tatggtgtct tttgttgact gcccccatgc
      181 atttacagca ataattgaaa ttgaaattaa gattagtact ttgttcatgc tgtcgtttta
      241 ttaataatca ggacaaaaat aagaataaaa taatttactg gctaatatct aagcatttat
      301 tgtgctggat taaaaaacgg aaaagcttaa tatgctcttt cgttgcgacc ctcaatataa
      361 ttgatgaatg atgtatttac aactttgttc ccgccgggag tagggtattc gccggtaaaa
      421 taccagtcgc cgtgattatt ccagcatgcc ttgtgaagat cttcgacccg ttggaaaatt
      481 atccccacct cagcatgtat agcctcatcg cgtatcaact ccgatatttt atgcgaaatc
      541 tgctcctcag tgaaagtatt gtatatatgg ccaacatagt taatcatttc ttctttagga
      601 aggtattgct gcgacttgca tttctgatac acattttcaa taacatgctc cattccgttt
      661 tctttaagca aaactattgc tgcttgaaaa gccacaaaat cgccgagttt tgccatgtcg
      721 attccgtagc aatcggggta acggatttga ggtgccgacg aaactataaa tatctttttt
      781 ggctgaagac ggtcgagtat acgaagaata ctctcgcgaa gtgtggtgcc acgaacaata
      841 ctgtcgtcga taattacgag ggtatcggta tgcggtacaa tggttccgta ggtaatatcg
      901 taaacatgat gaacaaggtt gttcctgtct ttatcgctgc ttataaatgt acgaagtttc
      961 atatctttga cagcgatttt ttcaacacgc ggacgaagcg aaaatatttt atctagcatt
     1021 tcatcattta gatgttcgcg attttccgaa ataatttttt tatttcgttg caatagataa
     1081 ttttcaaggc ctttcagcat accataaaat gcagtttcag ccgtgttagg aataaacgaa
     1141 aacactgtgt tctcaaaatc atgattaatg ctttccaata ttttgggtac aagaagttct
     1201 ccaagttttt tcctttcgtt gtaaatgtcg gtgtcggtac ctcgagagaa gtaaatacgt
     1261 tcaaaagagc agggagtttt tacccggggt acgcgaatca agctgtgcaa tgtatcaccg
     1321 tttttcttga taattagagc atgaccaggt tcaacttctt tgatttcgtt gaagggaaca
     1381 ttgaaaactg tttgtattac cggcctttcg gaggctgcaa cggcaatttc atcgttttta
     1441 aagtaaaaag ccggacgaat gccccatggg tcacgtataa tgaatgtatc gccatgtccc
     1501 agcataccag ccatgacata tccgccatcc cagttttggc tggcactggc aagaatttta
     1561 accaggtcaa tgtttttttc gattaatggc gaaatgtcct tgtgagcaat tcccctgttt
     1621 ttataatccc tgaaaagcct ctgattttct tcatcaaggt gcaaacctac catttcgagt
     1681 gcggtaacgg tgtcggaata atcgcgaggg ttttgtccga aatctaccag atatttaaac
     1741 agttcgtcca cattggtcag gttaaaatta cctgccataa cgagtgtacg tgagcgccag
     1801 ttgttctgtc gaattacagg gtgggcattt tcaatgtcgt ttttgccaaa agttccatag
     1861 cgtaaatgcc ccatatatag ttcacctgca aaatcaaagt ttctttttgc ttcattacag
     1921 tcggtaatgg ttccatttcc attaagtgct agttgggtca ggttttccga tatctgctca
     1981 aaaatatgct ggatgggatt tattcttacc gatctgaagc gattgatgta tttgtttccg
     2041 ggatccatgt ccgttttaat gcaaacagct cctgctccat cctgcccgcg gttgtgctgt
     2101 ttttccatta aaaggtacag cttgttcaac ccccattgcc atgtgccgta tttttcctga
     2161 taatattcca aaggttttaa caggcggata acagcaattc cgcattcatg ttttatggcg
     2221 tcactcattt ttttgtcttt atcggtttgg taaaaacgcg aaacaaagct ataagttttt
     2281 atcggagttt tgtaactttt tagtgagagt tgaaaacttt taataagaat ggcccaacag
     2341 cagtgcctaa tagaatatag gtttgtgcaa aaacggttta acattaggtt gttgtggtca
     2401 gaaagagatt ataaaaagtt atcattcaac ttgtaaatga tattataaaa tgtgctatct
     2461 ttatagcctg aaaatcaaaa ctatgaaacg agctctactt tttgaattca taatggtatt
     2521 atttgtaggt atacaagccc agactatcaa taatagcagt acaaaagtca atattccaga
     2581 tggtacgtat gttattttca gcaacttgat aaactctggt tccggtgctg aatttaatta
     2641 taataatgat ttaaccattt cgggaaactg gacaaattct aatgcagcag tttttacaag
     2701 cggcccgaat agttcggtcg tttttaacgg atcgtctcaa caaaccatca caagtggcgg
     2761 tagtcagttt cgcaatgtag taattaataa tacatcaact actgactatg ctataatttt
     2821 gggtgacgac atgtcaatat ctgctcaact caccctgact gatggtgtta ttaatggagg
     2881 aacgtatcag gttaaaattc ttaatgaggc tacctccagc gcaggaagtg cgattagtta
     2941 tgtcgatggg attgttgcaa aaacgggcac agttgccttt acttttccgg tgggtgacgg
     3001 gctggtttgg gctcctgtcg gaattgcagc tccttcaaca agctcaacaa tatcggcaca
     3061 atacactttt agcgaaggcc cattaaattg gtcggcttct tatatgtgcg acgaaagtca
     3121 gatgcaatat accagtggtg tggaacactg ggatatggca acaacttcgg caactcctgc
     3181 cgtgacgtta tattggaaaa atggaacgcg aagtggtatc actgatctta ctgatctggc
     3241 tgtggctcat tttaacggaa cctgttggga gtatatggaa ggtactacta ccggaaacac
     3301 tagcgatggg tctattacat ctaccctgcc gttttcttcg tacagcccgg tgagttttgg
     3361 cagtaaaaac cgcatcaatc cgttgcccat cgaactgcta agtttttcat ccaattgtac
     3421 cggttatggc gtgagtctgg aatgggtgtc tgcctccgaa tcgaatagtt atggttatta
     3481 tatcgaaaaa tcgctcgatg cagttaactt caaagaaatt gggtttgtag acgctgccgg
     3541 taatagtaat caggttctta attattcctt caccgatgaa aatgtagaaa aacaagccaa
     3601 gtactatcgg ttaagaatgg tggatgtcga cgggcaattc aagtatagca gcaccattgt
     3661 agcattttgt tacgaagcag gggcaccggt tattatggtg tacccaaacc cgttcaaaga
     3721 cgacctcaat ataatcggag aaaatttccg ggacgaaaac acaataatct acgtttacga
     3781 catgcttgga aaaattattg ctgaatataa agttgaaaat ttaaacgggt ctttctctca
     3841 aattataaat atacatgatt tgagtccggc aatgtatact gttaaaatta tttccgggtc
     3901 ctttaaccgg attgtgaaag ttacgaaaga acaatagcgt aggtttatta tacgatccat
     3961 aattgccaat tttttgggtt gttttatgta ttaatccata ttatgaaaac aaaaattgca
     4021 tttctttttt tcgttgttat tatgttatat gtaacaacaa tcaatttgag agctcagggt
     4081 gttgctatta acctcgatgg aactgcagcc gatgcatcat caatgctcga tgtgaaagct
     4141 acaaacagag ggttgcttat tccccgtgtt gcattaacac agacaacctc ggctagtccg
     4201 gtatcgaacc cgacagccag tctgatgatt tataacactg catcaacggg agacgttact
     4261 ccgggatatt actattggag cggctcagcc tgggtgcgtt tcggaagtgc agtgcatacc
     4321 catgcaactc ttacgcaggg aacgggaatg aacactttta gttataatgg aagctctagc
     4381 gctacggttg ctttggcaaa tactacagtt actcccggta cttatggaag tgcctctcag
     4441 gtgggtacat ttactgtcga cgctcagggt cgattgacat ctgcctcgaa tgtaaacatg
     4501 acaatgccaa ccagcacggg agcactaaat atggtttatc cggtttatat tcgtggaaca
     4561 ggtttaaata ataatgcaaa tcaggtttta atagtaggaa gcacaactat ttataataca
     4621 ggagcgagag gtttacgatt aaccgtattg agcaaaaccg atttttcggt ggttagcgat
     4681 gcaacatacg acacttatgg cagtaccact gcatccgata atcttgcaat tgctcttaat
     4741 ggattaaata atagtcaaat tggtgtttta accagctggg acgcatggga gggagctgtt
     4801 acaaccaatc ttgaaaatgc gttcaaaaga cttggtttgt ataaagcgta tgccacggtt
     4861 aacagtggat cgcgacgcac ttacagtgcc atcttcgaag ctgcatcctc aggcgaaacc
     4921 accgataaag cggttgaagt ttcaacggat aataccgcaa atcagcctta tgcagaaatt
     4981 aggggttggc tgattaacgg ttcatttgtt gcatctggtt cccagaaaaa tgcacttacc
     5041 acaccacaag gaacagcatt aggattaatt gttgacccat cgggttatgt tggcattgga
     5101 actgacaatc ccggatcaag gcttgatgtg tcgggaggca atgttcgcac caccaatcag
     5161 ttcgtttcaa caatagctac agggaatgcc cctattgtgg tttcttcaac tacgctaaat
     5221 acaaatctga actccgatat gttggatggg ttacatttga attcaaccac aacaaacaat
     5281 caggtcaatg cagttgtacg taccgatgct aacggatatg ccaattttgg gtggattaac
     5341 acaataagtg gcgataatgg aacaaccgcc ataagcagaa tatatgcttc taacgatgca
     5401 tatatacgat attatactcc ggcaaacttc attactgcaa tgggtttgca tacgggcagc
     5461 ggttcggcaa actatctggc cagatggaca ggttccacaa cccttggaac gggtgttact
     5521 tacgataatg gtaccaatgt tggaattgga aattacagcc cggcctataa gcttcatgtt
     5581 ggctccggga atggagacgg tattatcatt ggcaattaca acgacaggct gggttgggac
     5641 gggtcagggg cacaaccgga attggcaatc cgatttgcag gataccgtga tgtggtgagt
     5701 aattttaccg gtgcaaaaat atcggcaatc agaaccaata tttgctgttc tgcattatcg
     5761 cagggcacag aattggcttt ttatacacag gaatctactg caattggttc aggcgactca
     5821 aacctgtcag aaaaaatgcg tatcggcagt ggtggcaatg ttggaatagg aacatcatac
     5881 ccaggtgaaa aattggttgt atctacttct tcaaccaaca ttgcagttta tggctataat
     5941 ggaagaagca cctcttcggc aaacaatatc ggagtggaag gtgtggcaac cggaacaaca
     6001 ggctcttctt accggaatat aggtgtatat ggttatgctg ccgacaatac gagtgccgaa
     6061 gactatggtg tattgggtag ttgtgcaaac accaccgatg actgggccgg attttttgcc
     6121 ggtgacgtat atgcctcaga ttattacgat attgctgaaa tgtattctga tgccggagaa
     6181 atacccgatg gggcattggt ttcattaatt cagccgatta atgaccctgc agatgcgaaa
     6241 gttcagattt cccagaatgc gtacgatcca ttattaattg gtgttaagtc agagaatccg
     6301 gcgttatata tcggaccaac tgaaagcgga ttagaggtga ttcctaaata tagagaatta
     6361 ttgaaaacgc gggaagaaat tctacacaag atttatatcg aacaggttgg tgagcaagct
     6421 gtggatttgc atcagcaatt agaaaccgtc gataacgaaa taagtcaatt attaattaaa
     6481 atgaatccca aaggtaaagg tgatgttggc tctccaattg ctttggttgg tagagtgaat
     6541 gtgttggctt gcaccgaaaa cggacctatt aaaccaggcg atccgttaac atcgtcaagt
     6601 aaacccggat acgctatgaa agctactaag ccctgtcaga ttgttggttt ttcaatgagt
     6661 gaattatcag caggcgaagg taagattgta cttaatgtta accacagttt ttataacccc
     6721 tttagtgaaa caacagaatc actttggcaa tatatttact cattggaaaa gcgaatagaa
     6781 ggtttagaaa acaaacaata aatgcaagcg aattaaatta tcgttttata ggagcatttt
     6841 tgtggtaaaa taattcctaa tccattgatt aataaatcgt cttacaattt cgacgcacat
     6901 attcaatata gagttacatt tgtattttaa tggattatta aactgaaaaa tatgaaaaca
     6961 caaaaagtac tctttttgtt ggcatgtatt atagtgtctt tcacaaattc cgtggttttt
     7021 gctcagggtg ttgccattaa cctcgatgga actgcagccg atgcatcagc aatgctcgac
     7081 gtgaaagcta caaacagagg gttgcttatt ccccgtgttg cattaacaca gacaacctcg
     7141 gcaagtccgg tatcgaatcc ggtagccagt ttgatggttt ataatactgc tacagcaaac
     7201 gatgtaaccc cgggttatta ctactggagc ggctctgctt gggtacgttt cggaagcgcg
     7261 gtgcataccc atgcaacttt aacacggggg gcgggtttga caggaaataa ctacgacggt
     7321 tccacaacca ccacatgggc tgttgatttt gggaatacta cagttgccgg aaacggacct
     7381 aaatcacagg gaaattttgg acaatttcag ccacatggaa cctacacgaa ttttaatacc
     7441 gaccctgctt actggggatg gaactatgtg cagggaaaca ccaatgctcc aaacacaaac
     7501 tcatcgcagt ggtaccgcga agtaatcagc ctcggcgatg cctattcggc acggggaaca
     7561 ggaggatact cccttgaact ggcatttcca cgatttagtg caagtactgc cggcatttgg
     7621 atgcgaactg tggaaaatgg tactatcggt gcttggacaa gaattgacgc taatggtatt
     7681 aactattcaa atcttgccgg aatacctgta agaaatgcat ggagtggggt gcatcgcggc
     7741 ttcgtagctg aacaattatc atggaaaaat tacggtaaca atcatacaat ttttgatgcc
     7801 tcgcaaggaa catcacctga tggaactgcg gtaaataata ccaattcaca agttgcatgg
     7861 acagcctcct atcctacgct tatgggctgg aatggcagca atacctatgg ggttagagtt
     7921 gatgttgccc gttatgcaga atctgcaggc tccgcacctg gcgacaatct gggtaatcat
     7981 acagcaacca ccactttaaa tatgaacaac aatcaaataa caaatttttc atatatgcaa
     8041 ccctatggtg ttggggggga ttccggtcag ggcaataatg cttatggaat atttcaggaa
     8101 ggcggttctt ggtcgagccc ttatcccgat ctgagaattg ctttccacac aggtataaat
     8161 ctgggggcaa atgcgtcgta tggtggaatt gggttttata cagactacga catgggaaca
     8221 ttggtaatgt cgataaatga tgcagtgacc ggtgccggtg caaacaatgt gtatatggca
     8281 ggtggcctta aagccggtag tgcttataat atcaacattc ccaattcatg tggcggcaac
     8341 tgggcaacaa attgcgattt ggccatctgg acagcagacc ataccggagg ctgcggcgat
     8401 gaggcttata tcgcgcatta tatacgcaca ggtgaagcga cgaccctcga aattggaaat
     8461 aaaaacgacg ctgatgatca tattgctctg atgccatcgg gcaatgtagg aattggaaca
     8521 actgcacccg accaaaaact ggtcgtgaat ggcgggaatg ttgatgtaac agctggtggc
     8581 ggcgggctca gattgggttc aaataattgg tctgatggtg gttctacata cggacacata
     8641 gttacagata atggctctta cgatgctctt atgatagtag gaaacaatac cgccggagat
     8701 ggacatagga atgtccgtat ttgggatcac ctgaacgtgg aagggactct tacccgtgga
     8761 ggtaataccg tttgggatgc agcaaatgat ggctccggtt ctagtcttga tgcggatctt
     8821 ctagatggga atcatgcttc ggcgtttgca gcttcaagcc acacccatcc atggaatcag
     8881 gtaaccagta aaccttcaac atggctcgat ggagcaacgc tgattgagga taatgctgat
     8941 tttaacaact caagacccag tggattttat caataccaca atgcagccaa tgctcctaca
     9001 ggcggaacat ggtataacat gattaatgta aggcatagta atactggcaa cgatcatggg
     9061 tttcaaattg cggcatgtta ttatgatgaa aacatttgga cacgtaccta tcaaggcggc
     9121 accggacata atgatggatc ttttacccca tggagagcat tgattcacac tggcaacctg
     9181 tcgtcaaatg caattttaaa tcagtattca ggtgctcaaa gcgcaaacat gtggatcagc
     9241 ggtgaaacca gaaccggggg gtggttcaga agcagtagcg gcagtacggg ttggtacagt
     9301 gaaccgtatg ggtctggaat ctatgccact acaagtggcg aggttgcaac ctataatggt
     9361 tcgtacttca ggataaccgg atctgcaaat ggctcaggca atctaaggtt tgatgcagcc
     9421 aatccttata tttatgcttc atcgtatttt ttggcacccg gaggcgccta ttttaacagc
     9481 ggaacggttt ataccgaggc tcaatatcag tgccgtggtg gtattcacaa tgacgcggct
     9541 ggcgatttaa ctattgccgg tggaaccaat ggaaccacct atttttcagg ccctatttcc
     9601 ggaatgagtg gtaccatacc gtcaaattat atggtacggt taaccccgaa tctgcatttg
     9661 aatgccggga cgggatacgc agttatagcc aattgggata atggcacaac cggcacgtct
     9721 caagcattgc gggttggaaa cggagcagga tctgatgcat tctatgtaag agccgattct
     9781 tatacatggg caggaacaac ttcaacttcg ttgtcaaatg taatttatgc atcaagacct
     9841 tctggtaatt atggcggcgg tagagcaaca atttatggtt atagatatgg tggtagttca
     9901 gggtcagatg gttcaagcta tagtgtttac ggaactgatg caggggttga gggctataca
     9961 ttttatggta atttgtactc ttttggggtt gctggtcata cctatggcga ttataacaga
    10021 accggaggta ctatgggagc ggataataat ggttcctact ggggttcgct tggttataag
    10081 aatagcggta gctcatggta tggattgtat tatacttctg cgggttctgg gggaggttat
    10141 atgaacaaca atggaattat atccggaatt ggttctggtg gttgcggtgg ggtaatgggt
    10201 ggctggagcc gcggcgaagt gcttggtttt accactgcag gcgaactata tgcttcgtat
    10261 aatatgggaa atgagtatac ttcaggtgtt tctgcagata ttgttaccac ctccacagaa
    10321 cgaatcccgg tttattcggt tacctcaaac gatgttaaag tctatgctga tggtaccgca
    10381 caactggtaa atggcaaatg ctttgttgca tttgatgaat catttgtgaa tgtcatttcc
    10441 aaggaaggaa aaccaacaat cacagttacc ccaaacggag aatgcaatgg attgttcacg
    10501 gttgatattt ctagtcgcgg ttttactgta aaagaattaa ataatggctc ttcaaccgtt
    10561 gagttctctt ggattgctat cggtaaaaga attgatgcag aaaaagccgg agatttgcca
    10621 caagcaatta aagacaaaaa ctttgataat aacctgaagg gtgtaatgtt caacgaaaac
    10681 gataaagaca attcagcgac tcccatctgg tgggatggaa acaacctgag gttcgacaaa
    10741 atgccagaaa caccggtgaa caagcccaaa gatgatccta atttgcccgc cgtaaataat
    10801 agcgatgacg taaaccgtct gattcagcaa aacaagcaat cgcaacccga aaacaaggaa
    10861 tggagaccta ctcccgaaga accgatacga attcagggaa atgagaaaaa ataagctaat
    10921 atatcatagt atatgacgta ttgtaaagcg ggaaaaaccc gctttatttt ttttatatgg
    10981 tagacaatgc atgaaatttt actcaatttt cagtttttaa aattaggttt aatataagag
    11041 taaaccctgc tataatgttt catagcttat ttttcattta tgccaaataa taccgtattt
    11101 ttgtgtaata acttaaccaa aatagtatga aa
//
LOCUS       DAOOQP010000099        10897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7530_length_10897_cov_5.621011, whole genome shotgun sequence.
ACCESSION   DAOOQP010000099 DAOOQP010000000
VERSION     DAOOQP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7530_length_10897_cov_5.621011"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLK75_09810"
     CDS             complement(<1..825)
                     /locus_tag="PLK75_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39582.1"
                     /translation="MISKSVSILISGLLFIGISLNLQAQITANGASGSFPTTYSTGFI
                     NNGGQNDLVYVFCGTQDEANVGSLTVSASGCSVAWFRYDGISFVPIGQTSATANNLTS
                     GCYMARVTCGTTINCYRAWVWVNQSYVEIDPIAPGCESFTLQGTAQIMDNSYTINDPP
                     GSNFEIDENTYIKVCFWATHTYVSDIGFYLKSPGNQPTEPGNPGVVQLCPAASDWGPG
                     AAQGSWTGIPWTTLGCSDPSDENTVCNSGNNVNNFCFATHTSPGGTALPAGNPTYTP"
     gene            complement(845..1267)
                     /locus_tag="PLK75_09815"
     CDS             complement(845..1267)
                     /locus_tag="PLK75_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39583.1"
                     /translation="MKEFSKIGFFCIMLTLIVQPAFSQENTLTEGRYIVITNSATWFN
                     EVNGYIEVRDFLLYNVNDDESSKPLFTEINEMSNIVKFSIGSSSEKIEHTRRCTVIMN
                     RDDYRNTFYKVLRKMQVKTVFVDGTAMSVEDFFMLINE"
     gene            1685..3970
                     /locus_tag="PLK75_09820"
     CDS             1685..3970
                     /locus_tag="PLK75_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY39584.1"
                     /translation="MDFLNDLNEAQREAVEYIDGSSLVIAGAGSGKTRVLTYKIAYLL
                     ECGYDAHQILALTFTNKASGEMKERIASLVGESLSHNLTMGTFHSVFSRFLRTEALNI
                     GFTKDFTIYDTADSKNLVKSLITELNLNDEYYKPNRIYAQISKCKNNLLPPGTYRQHD
                     TLLEADAKARIPQFIDLYDAYCKNCRKSNVMDFDDLLYYTNVLFRDFPGVLAKYADRF
                     RFILVDEYQDTNLAQYIIIKKLASVHRRLCVVGDDAQSIYGFRGARIENIFNFQSDYP
                     EYKLFRLEQNYRSTQNIVDAANSVIKKNSRQISKTVFSRNEEGQPVKVMKALTDHEEG
                     IMVAHAITESIYNNRIPYSEIAVLYRTNAQSRIIEEALRKLNVPYKIYGGLSFYQRKE
                     VKDVLAYLRILINQKDNEALRRIINYPSRKIGVATVDKLFSLAEDNELSIWEVIRNEK
                     LLHDSDFNTGTITKLSKFAELINELRGQIEELNAADLLDLVIRRTGIKHELMEDKTPQ
                     GVSRYENVDELINAARTYVDEQSVVDPNQLVTISSYVENVSLLTDQDSDNKEDFPKVT
                     LMTIHSAKGLEFEHVFLAGIEEGLFPNAMSLESELELEEERRLFYVAITRAKKRLTIT
                     YCGSRYRFGSIAYPNPSRFITEIDPKFVSWAETSRNAHKTTPENIVVSTPANAQSRLI
                     SLKSHDLTGGGVAAPQQHDNEFMHPDQVVPGIDVLHDRFGKGKVISTDSYGNDKTAVV
                     FFENLGNKKILLKFARLSAIK"
     gene            4016..4624
                     /locus_tag="PLK75_09825"
     CDS             4016..4624
                     /locus_tag="PLK75_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOY39585.1"
                     /translation="MDYNTQRSKLIFPEYGRHVHRMVDYLKQIDNKADRTRNAYAVIS
                     IMGSMHPHLRDVPEFTHKLWNHLAILADYELDIEWPFPLPDKEELLSKPDMIHYSDNK
                     IAFKHYGKNVENMLKKVNDYENPEERDALITLIANHMKKLFFTWKKEHIEDTVIFEDI
                     RRLSGNAISIPENLKLNEAWDMYALSGQTNNRTRKKKQFKKK"
     gene            4641..5948
                     /gene="murA"
                     /locus_tag="PLK75_09830"
     CDS             4641..5948
                     /gene="murA"
                     /locus_tag="PLK75_09830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOY39586.1"
                     /translation="MPTFKIEGGHRLKGSILPQGAKNEALQVICAVLLTDEPVKISNI
                     PDIADVNKLIELLRDLGVKVTPVAKGEVVFEAKNINIDYLESTEFRKKGSSLRGSIMI
                     MGPLLARMHSVAMPKPGGDKIGRRRLDTHFFGFIKLGASFDYSYKDDFYRMFADELRG
                     SYMHLDEASVTGTANIIMAAVLAKGLTTIYNAACEPYIQQLCNMLNSMGAKITGIGSN
                     LLYIDGVSSLHGTNHRILPDMIEVGSFIGMAAMTHSEITIKDVSYDNLGIIPAQFSRL
                     GIQMERIGDDIFIPAQERYEIETFIDGSIMTIADAIWPGLTPDLLSVFLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLDNQIKLRGIQMSSPDIRAGM
                     ALLIAAMSAEGVSTIHNIEQIDRGYEQIDVRLSQLGANIKRIE"
     gene            5963..6655
                     /locus_tag="PLK75_09835"
     CDS             5963..6655
                     /locus_tag="PLK75_09835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY39587.1"
                     /translation="MKKIHLFFIAVVIGLSAVAQQQPVTGLNIGQTAPEISLPDVNGD
                     TISLSSLRGKVVLIDFWASWCNPCRKENPNVVRAYRKYGEKKFTVGNGFTVYGVSLDK
                     AADAWKAAIDKDSLAWVNVSDLKYWSSVAAAAYSISSIPSNILIDKNGVILARNLRGP
                     ALEETLEKYVLPDPIELLVNQKKSMDKAIEMMKQSEDYSKYSKNIKNLEKKLAALQKE
                     IDALQSLVKPVK"
     gene            6814..7395
                     /locus_tag="PLK75_09840"
     CDS             6814..7395
                     /locus_tag="PLK75_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOY39588.1"
                     /translation="MQQDGQNDMKDAVNSNLEHDDNSVESKGEQNETSRKSRKLRKRE
                     KEEDVLKKEISELKDKHMRLQAEFDNYRKRAMKDRIDLMRTAGEDLLVKLLPILDDFD
                     RAINHIEKADTLEGFKEGVFLIAGKFRDFMKQNGVAEIEAKGEVFDTDFHEAVTQYPA
                     PSDDLKGKVVDVAEKGYKLHDKVIRFSKVIVGV"
     gene            7405..8553
                     /gene="dnaJ"
                     /locus_tag="PLK75_09845"
     CDS             7405..8553
                     /gene="dnaJ"
                     /locus_tag="PLK75_09845"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOY39589.1"
                     /translation="MAKRDYYEILEVAKNASAEEIKKAYRKKAIQYHPDKNPDDKSAE
                     EKFKEAAEAYEVLSNPEKRQRYDQFGHAGVSSGGGFSGGGMSMDDIFSQFGDIFGDSF
                     GGFGSFFGGGSRRGGQKRVNKGTNIRIKVKLDLIEIASGTTKKIKVNKYVSCSHCNGT
                     GEHGGNAHSTCHTCNGTGMQTRVTQTILGHMQTSSPCSACGGEGKVITQKCTHCYGEG
                     IVQGEEVIEIRIPGGVSDGVQLSMSGKGNAARRGGVNGDLIILIEEEEHPEFVRDGND
                     LLYNMFIGIPDAIIGNAVEIPTLDGKVKIKIDPGTQSGKIVRLRGKGLPDLQGYGKGD
                     LLVRINVFIPSNLNRDERKIIERLQSSESFDPAKEPKSKLFDKFKGIF"
     gene            8932..10143
                     /locus_tag="PLK75_09850"
     CDS             8932..10143
                     /locus_tag="PLK75_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOY39590.1"
                     /translation="MKWYPLFFLLLTIAPNLINAQIKVLNDGNVGINCSTPLESLQIG
                     DRWTFYNGSPKVIGYNFYYDGSYSKRILADEVGLLKFEANGDIKFSFAGYGSAGSTIS
                     SFTDGLVLKNSGRVTIGSLDNGRFTVSDNSNLTPITAYANHSVDWTYGLISLVNRVNT
                     KAICVYYNSGDQFVVYGDGTTWTRDLIEYSDLTLKENIETINNPLEKVLQLNGVYFTY
                     KITELAADADSNTVISDEPPHRQMGLIAQEVEEIVPEVVNTNENGLKGVSYQNLIALL
                     IEAIKELEVQVSALENELNECCNSSDVNKKSSNIIESQSNLSGCYLLQNRPNPFDNET
                     IIEYYIPLERANASMLFFDMNGKLLFSKDVDLNKGNLTINRGELMPGMYYYTLIVNDE
                     EIDTKKMILTN"
     gene            10153..>10897
                     /locus_tag="PLK75_09855"
     CDS             10153..>10897
                     /locus_tag="PLK75_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39591.1"
                     /translation="MRSIKFILIISLCFCTKIYAQDNIVQIWLNDSTILSTQPFNHYN
                     EIYWGPPVDITAGGGAYSTSGFNNTNDIVTQLGDNGGQGYAALICDTLTAGGFSDWYL
                     PSRFELITIYHKADSVGGIFTFYSENTPFVWSSTEWMDTSALCFVSGMESYAVNKNEL
                     ANVRCVRRDTVSGINSIADNVFKITYNNETRELYVSAFNGSGNINIAIYDANGKQLFS
                     KTSRLYVSSSSERISLSAFTPGICITFFFV"
BASE COUNT         3444 a         1995 c         2375 g         3083 t
ORIGIN      
        1 gggggtatag gttggatttc ctgcgggaag ggctgtgccg ccaggcgatg tgtgggtggc
       61 aaagcaaaaa ttgttaacat tattaccgct gttgcaaacc gtattctcgt cgcttgggtc
      121 actacaaccc aaggttgtcc aaggaatgcc agtccagcta ccctgagcag cacccggacc
      181 ccaatcggaa gctgccggac ataattgtac tacaccaggg tttccgggtt cagtaggttg
      241 atttccgggt gatttcaaat aaaacccgat atcggaaaca taggtgtgag ttgcccaaaa
      301 acatactttg atatatgtgt tttcatcaat ttcaaaattt gatccgggag ggtcgttaat
      361 tgtgtaagaa ttgtccatga tttgggcagt accttgcagt gtaaatgatt cgcatcccgg
      421 ggcaattgga tctatttcaa cataactttg gtttacccag acccatgcac ggtagcaatt
      481 aattgtggtt ccacaggtaa ccctagccat atagcaacct gaagtcaggt tgtttgctgt
      541 agcagaagtt tgcccaatcg gaacaaaact gatgccatcg taacggaacc atgccacact
      601 gcatcccgaa gcactgacgg taagtgagcc aacatttgcc tcatcctgtg tgccgcaaaa
      661 aacatacact aggtcgtttt gcccaccgtt gttaataaat cccgtactgt aagtggtagg
      721 gaatgagcca ctcgcgccgt tggccgtgat ctgtgcctgt agattcagac tgataccaat
      781 aaacagcagt ccggaaatta aaattgatac acttttggaa atcatattgt atcggttttt
      841 attattattc attgattaac ataaaaaaat cttctacgct cattgctgtg ccatcaacaa
      901 aaacagtttt tacctgcatt tttctgagca ctttataaaa cgtattcctg tagtcatctc
      961 tgttcataat tactgtgcat cgccttgtat gttcaatctt ttcggaagaa gacccgatgc
     1021 tgaattttac aatattgctc atttcattta tttcagtaaa gagcggtttc gagctttcat
     1081 cgtcatttac attgtataat aaaaaatcgc gcacttcaat gtatccgtta acttcattaa
     1141 accatgtggc agagttcgta attacaatat acctgccttc agttaaggtg ttctcctgtg
     1201 aaaaagcagg ctgcacgatt agcgttagca taatgcaaaa aaagccgatt ttactaaatt
     1261 ctttcatatt gtgttgtttt gaattattca ttagctctaa aactgcgaaa tttaattata
     1321 attttcaaaa gttcaaaata aagacaaatc tgcacactga aacgttgcct gttttggtaa
     1381 aattgaacac tggtttcgtt aaaaattatt ataaaatgcg atttatttga gatgaaaaat
     1441 aagcgaactc caatggtagg ataggttttg aataattata ataattgtaa aacaaagctt
     1501 tgataaatta aaatttcata tcgtttttgg agaggtgtac agcatcatat ttgctgtatc
     1561 tggttcaaaa gaagaataac aattgcaaaa tgttggcttt ccggactaag gaaatgcctg
     1621 agaaaattat taacaatagg aaaaactaca aactatagat ataattttgc caaaaaaaac
     1681 ggtgattgat tttttgaatg atttgaatga ggctcaacgc gaagcagttg agtatattga
     1741 tggctccagc cttgtgattg caggtgcggg aagtggtaaa acaagagtac tgacatacaa
     1801 aatcgcttat ttgcttgaat gtggatacga tgcgcaccag atattagcgc tcacttttac
     1861 taacaaggct tcaggcgaaa tgaaggagcg cattgcttca ctggtaggcg aaagcttatc
     1921 gcacaatctt acaatgggga catttcactc ggttttttcc agattcctcc gcacagaagc
     1981 tttgaatatt ggatttacta aggattttac tatttatgat actgctgatt ctaagaatct
     2041 tgtaaaatcg ttgattactg agctgaacct gaacgatgaa tactataagc ccaatcgcat
     2101 ttatgcgcaa atatcaaagt gtaaaaacaa tcttttgcct cctggcactt acaggcagca
     2161 cgatactctg ctggaagctg atgccaaagc acgaatacca cagtttatcg atttgtatga
     2221 tgcatactgt aaaaattgcc gtaaatcgaa tgtaatggat ttcgacgatc tgttgtatta
     2281 cacaaacgtg ttattcaggg atttccccgg tgttttggcg aagtatgccg atagattcag
     2341 gtttatactc gtggatgagt atcaggatac caatcttgcg cagtatataa tcattaaaaa
     2401 gcttgcatct gttcatcggc ggctttgtgt tgtaggcgac gatgcccaga gtatatatgg
     2461 cttcagggga gcacgtatcg aaaatatatt taattttcaa tccgattatc ctgaatataa
     2521 acttttcagg cttgaacaga actatcgttc tacacagaat attgtggatg cagccaactc
     2581 cgttataaaa aagaactcgc gtcagataag taaaacggta ttctcccgaa acgaagaagg
     2641 gcaacctgtt aaagtgatga aagcgcttac cgaccacgaa gaaggtatta tggtggcgca
     2701 tgcaataacc gaaagcattt ataataatcg tataccatat tccgaaatag cggtattata
     2761 tcgtaccaat gcacagtcgc gtatcatcga agaagcattg cgtaagctaa atgttccata
     2821 caaaatttat ggaggccttt cgttttacca acgcaaagaa gtgaaggacg tacttgccta
     2881 cctgaggatt cttatcaatc agaaagataa tgaagccttg cgcagaatca ttaattatcc
     2941 cagtcgcaaa atcggtgtgg caactgttga taagttgttt tcgcttgccg aagacaacga
     3001 actaagcatt tgggaggtaa tcaggaacga aaagctgttg cacgattctg acttcaacac
     3061 aggtaccatt actaagttat cgaaatttgc cgaattgata aacgaactca ggggacagat
     3121 tgaagagcta aatgccgctg acttgctgga cctggttatc aggcgtacag gaattaagca
     3181 tgaattgatg gaagataaaa cacctcaggg tgtaagtaga tacgaaaacg tagatgaact
     3241 gattaatgcg gccagaactt acgttgatga acaaagtgtg gttgacccga accaacttgt
     3301 tacaatttcg tcgtatgtcg aaaatgtcag ccttcttacc gatcaggatt ccgacaacaa
     3361 ggaagatttc ccgaaagtta cactgatgac tatccactca gcaaaaggac tggaatttga
     3421 gcatgttttt ttagccggaa tcgaggaagg acttttcccc aatgccatgt cgcttgaaag
     3481 tgaattagaa ctggaagaag aacgccgctt attttatgtt gctattacgc gtgcaaaaaa
     3541 aagattgaca ataacctatt gcggatcgcg ataccgtttt ggttcaatag catacccgaa
     3601 tcccagccgg ttcattacag aaatcgaccc gaagtttgtc agttgggcag aaacaagccg
     3661 taatgcgcat aaaacaactc ctgaaaatat tgtggtttca acaccggcga atgctcagag
     3721 ccggcttatc agcctgaaat cacatgacct gacgggtggt ggcgttgctg ctcctcaaca
     3781 acatgataac gaatttatgc accccgatca ggtggtgcca ggaatcgatg tgttacacga
     3841 caggtttggt aaaggaaagg taatatcaac cgattcatac ggtaacgata aaaccgctgt
     3901 ggtctttttc gagaaccttg gaaacaagaa gattctgctt aaatttgcca ggctcagcgc
     3961 aatcaaataa ttttgaatta taattaatta aagtagtttt gtaaaaactt tcgctatgga
     4021 ttataacacc caacgcagta aacttatttt ccctgaatat ggccgtcatg ttcaccgaat
     4081 ggttgactat ctgaaacaga ttgataataa agccgacaga acaagaaatg cctatgctgt
     4141 gatttcaatt atgggaagta tgcacccgca tctgcgcgat gtacctgaat ttacacataa
     4201 actctggaat caccttgcca ttttggcaga ttatgagttg gatattgaat ggccgttccc
     4261 gctgcctgat aaagaagagc tgctgagtaa acccgatatg atacattatt ctgataataa
     4321 aatagccttc aaacattacg gaaagaatgt ggaaaacatg ttgaaaaaag tgaatgatta
     4381 cgaaaatccc gaagagaggg atgctttaat cacactcatt gcaaatcata tgaaaaagct
     4441 cttttttaca tggaaaaaag aacatatcga agatactgtt atttttgaag acattcgtcg
     4501 tttgtcgggc aatgctatat cgattcccga aaacctgaaa cttaacgaag cttgggatat
     4561 gtatgctcta tcggggcaga ccaataaccg cacaagaaag aaaaaacaat ttaaaaagaa
     4621 ataattaagt tctgcttgct atgcctacat ttaaaattga gggtgggcac cgtttgaaag
     4681 gaagcattct gcctcaaggt gccaaaaacg aagccctgca ggttatttgc gcggtattac
     4741 tcactgatga accggtgaaa ataagcaata tccccgatat cgccgatgtg aataaactga
     4801 ttgaattatt gagagatctg ggtgtgaaag ttacaccggt tgcaaaaggc gaggtggtgt
     4861 tcgaagcaaa gaacatcaac atcgactatc tcgaaagcac agagtttcga aaaaaaggca
     4921 gctcactacg tggttcgata atgataatgg gtccgttgct ggcgcgtatg catagcgtgg
     4981 ctatgcccaa acccggtggt gataagattg ggcgccggcg gcttgatacc catttttttg
     5041 gatttataaa actgggtgcc agtttcgatt acagttacaa ggatgatttc tatcgcatgt
     5101 ttgccgatga actcaggggt agctatatgc acctcgatga agcgtcggtt acgggaacgg
     5161 caaatattat tatggctgct gtgctcgcta agggacttac caccatttac aatgcagcct
     5221 gcgaacctta tattcagcaa ttgtgcaata tgctgaacag tatgggcgcg aaaatcaccg
     5281 gaataggttc taaccttttg tatatcgacg gagtgagctc acttcacggt accaaccatc
     5341 ggattttgcc cgatatgatc gaagtcggca gttttatcgg tatggcagct atgacccatt
     5401 ccgaaatcac tataaaagat gtatcttacg ataatctcgg aattataccg gcacagttca
     5461 gcaggcttgg aattcaaatg gaacgaatag gtgatgatat tttcattccc gcacaggagc
     5521 gttacgaaat cgaaactttc atcgatggct caattatgac cattgctgat gccatttggc
     5581 cgggacttac ccctgacctg ttaagtgttt ttctggttgt ggcgactcag gcaaaaggca
     5641 gcgtacttat tcatcagaaa atgttcgaga gccgtttgtt ttttgtcgac aagttgattg
     5701 atatgggtgc tcaaatcata ctttgcgatc cgcaccgggc cactgtcatt ggcctcgaca
     5761 atcagatcaa actgcgcggt attcagatgt cgtcgcccga tatcagggcg ggtatggcct
     5821 tactcattgc agccatgtca gcagaagggg taagcacaat tcataacatt gaacaaattg
     5881 atcgtgggta cgaacaaatt gacgtccgac tgagtcagtt gggcgccaac ataaaaagaa
     5941 tagaataaac taaaacgtct ctatgaaaaa aattcatctg ttttttattg ctgtggtaat
     6001 tggactttca gccgtggctc aacagcaacc agtaacgggt ttaaatatcg gacaaactgc
     6061 acctgaaata agcttacccg atgttaacgg tgatacaatt agcctgtcgt cgcttcgcgg
     6121 aaaagtcgta ctcatcgatt tttgggcatc atggtgtaac ccttgccgta aagaaaaccc
     6181 aaatgtggta agagcatacc ggaaatatgg tgagaaaaaa tttaccgtag gcaatggctt
     6241 tacagtatat ggcgtttctc tcgataaagc agccgatgca tggaaagctg caatcgataa
     6301 agacagcctt gcatgggtta atgtgagtga cctgaaatac tggagctctg ttgcagctgc
     6361 agcatatagc ataagcagta ttcccagcaa tattctgatc gacaaaaacg gcgttatcct
     6421 tgccagaaac ctcagaggtc cggcattgga agaaactctt gaaaagtacg ttttacccga
     6481 cccgattgaa cttctggtga atcagaaaaa atctatggac aaggccatcg aaatgatgaa
     6541 acaatcggag gattacagca aatattcgaa aaacattaaa aatctggaga aaaaactggc
     6601 tgcattgcag aaagaaattg atgcgttgca atcgctggta aaaccggtaa aatagattta
     6661 ccgtgatttc tgtcagaatg gcataaaatc aattttggct tactgtttgt tgaatatgca
     6721 cggtttaatg tcaatatcac tttgtggtaa tgagtatagg tgtttgttta tgattaatga
     6781 tgttaaaatg gcttaataaa aaagatgaaa gatatgcagc aagatggaca aaacgacatg
     6841 aaggatgcag ttaatagtaa tttggaacat gatgacaatt ctgtagaaag taagggtgag
     6901 cagaacgaaa catcaagaaa atcgagaaaa ctcaggaaaa gagaaaagga agaggatgta
     6961 ctgaagaaag aaatttctga gctgaaggat aaacatatga ggcttcaggc tgagttcgac
     7021 aattacagaa agcgggccat gaaagacagg atcgatctga tgcgtacggc cggcgaagat
     7081 ttactcgtta agctgttgcc aattcttgat gatttcgaca gggctataaa tcacatcgaa
     7141 aaagctgata ctctggaagg ctttaaagaa ggcgtttttt tgattgccgg caaattccgc
     7201 gattttatga aacagaatgg cgttgcagaa attgaagcca aaggggaggt gttcgacacc
     7261 gattttcacg aagcagttac gcaataccca gcgccttccg atgatttgaa aggcaaggta
     7321 gttgatgtgg cagaaaaagg gtataagttg cacgataaag tgatacgttt ttcaaaggtt
     7381 attgtagggg tataaattgg ttgtatggca aaacgcgatt attacgaaat tctggaagtt
     7441 gcaaaaaatg cttctgccga agagataaag aaagcttacc gcaaaaaagc aattcaatat
     7501 catcccgaca aaaaccctga tgataaatca gcagaagaaa aattcaagga ggctgccgaa
     7561 gcctatgagg tgctcagtaa cccggaaaag cgtcagcgct acgatcagtt cgggcatgcc
     7621 ggtgtaagct ccggtggcgg cttcagcggt ggcggtatga gcatggatga tatcttttcg
     7681 caattcggtg atatttttgg tgactccttt ggtggttttg gcagtttctt tggtggcgga
     7741 agtcgcaggg gaggccagaa gcgcgttaat aaagggacaa acatcagaat caaggtgaaa
     7801 ctggatctta tcgaaatagc ttcaggaaca acaaaaaaga taaaagtcaa taaatatgtt
     7861 tcgtgttcgc actgcaacgg caccggtgag cacggcggta acgcacactc aacctgccac
     7921 acctgtaatg gaacgggaat gcagacgcgt gtaacccaaa ccatattggg ccacatgcaa
     7981 acaagttcgc cttgctcggc atgtggtggc gagggaaaag tgattaccca aaaatgcacc
     8041 cattgctatg gagaaggaat tgttcagggc gaagaggtta tcgagatccg aattccgggc
     8101 ggtgtaagcg acggcgtaca actttcgatg tcgggtaaag gcaatgctgc ccgcaggggt
     8161 ggggttaacg gtgatttgat tattctcatc gaagaagagg aacaccccga atttgtgaga
     8221 gacggtaacg atttgctata taatatgttt attggtattc ctgatgcaat tattgggaat
     8281 gctgtcgaaa ttccaaccct cgatgggaaa gtgaaaatca aaatcgaccc cggaacacag
     8341 tccggcaaaa tagttcgact tcgcggcaaa ggattgcccg acctgcaagg ctacggtaaa
     8401 ggcgatttgc tggttaggat taatgtgttt ataccttcaa atctgaatcg cgatgaacgc
     8461 aaaattatcg aacgcctaca gtcgtcagag agcttcgacc cggcaaaaga accaaaatcc
     8521 aaacttttcg ataagttcaa agggatcttt taagctttaa cgaaatagtg taatatacga
     8581 agctttttga tacccttaat tgctaaagca aactttataa gtcaaaataa gggtgtaaac
     8641 gaatgctttg caatgctaat ttagctatgt ccttaagcaa caaacttaag tattaggggt
     8701 ggaaatttga taaatgaaaa ttgcttatac caattatttt gtattgtatt gtgagtttgt
     8761 aatccttaga gggaatcctt aagaaatttt tcacacaagt ggcaacaacc cattacattt
     8821 gaattgtcgt tcatttttga agtgattata ttattacctc aaaatatatt gtttttattt
     8881 ttatatataa attgtttata ttcgcaatta atcatcttaa taaacaattt tatgaaatgg
     8941 tatcctcttt tcttcttatt attaacgatt gcccctaatc ttataaatgc acagattaag
     9001 gtgcttaacg atggtaacgt tggtataaat tgtagtaccc ctcttgagtc tttacaaatt
     9061 ggcgatcgtt ggacttttta caatggctct ccaaaggtaa taggttataa tttttattat
     9121 gatggctctt attcaaaaag aatacttgct gatgaggtcg gtttgctgaa gtttgaagca
     9181 aacggtgata ttaagttttc atttgctggt tatggttctg ctggttcaac aataagttca
     9241 tttactgatg gtttggtgtt gaaaaacagc ggaagggtta ctataggttc gcttgataat
     9301 ggaagattca cggtatccga taatagtaac ctaactccaa ttacagctta tgcaaaccac
     9361 tcggttgatt ggacatacgg attaatttca cttgtaaata gagtaaatac taaagcaatt
     9421 tgtgtttatt acaatagtgg agatcagttt gtggtatatg gagatggaac cacttggaca
     9481 agagacctta ttgagtatag tgaccttact ttaaaagaga atattgaaac tataaacaat
     9541 ccgcttgaaa aagttttaca gctaaacgga gtctatttta cttataagat aactgaattg
     9601 gctgctgatg cagattctaa taccgtaatt tcagatgaac caccccatcg acaaatggga
     9661 ttaattgctc aagaagttga agaaattgta ccggaagtag taaatactaa cgagaacgga
     9721 ctcaaaggag tttcatatca aaacttgatt gctttgctga ttgaagcaat taaagaactt
     9781 gaagttcagg ttagtgcgct tgaaaatgag ttgaacgagt gttgtaactc ttctgatgtg
     9841 aataaaaagt cttcaaatat tattgaatct caaagcaatt tatctgggtg ctacttgttg
     9901 caaaacagac ctaatccgtt cgacaatgaa acaataatag aatattatat cccattggaa
     9961 agagccaatg cgtctatgct gtttttcgat atgaacggga agctactttt ttccaaagat
    10021 gttgatttga acaaaggcaa tttaactata aaccgaggtg aacttatgcc gggaatgtat
    10081 tattacacgc ttatagtaaa cgatgaagaa atcgacacaa aaaaaatgat attaacgaat
    10141 taatttttta ttatgagatc aattaaattt atattaatta tttcattgtg tttttgcacc
    10201 aaaatatatg ctcaggacaa tattgtacaa atttggttga acgattctac aatattaagt
    10261 actcagcctt tcaatcatta taacgaaatt tactggggac cccctgtaga tattaccgca
    10321 ggcggggggg catacagtac cagcggtttt aataatacca atgatattgt tacccaattg
    10381 ggcgataatg gtgggcaggg ctatgcggca ctaatatgcg atacgcttac ggcaggaggt
    10441 ttttccgatt ggtatttgcc atcaagattt gagctaatta caatatacca taaagccgat
    10501 tcggttgggg gtatttttac gttttattct gaaaacacac cttttgtatg gtcaagcaca
    10561 gaatggatgg ataccagcgc tctttgtttt gtttctggta tggaaagcta tgctgtaaac
    10621 aaaaatgaac tggcaaatgt acgatgtgtt cgaagagata ctgtttcagg aataaacagt
    10681 attgcggata atgtttttaa aattacatac aataacgaga caagggagct ctatgttagt
    10741 gctttcaatg gatcgggcaa tatcaacatt gccatttacg atgcaaacgg aaaacagttg
    10801 ttctcaaaaa cttcacgact gtatgtatca tcttctagcg aaagaatcag cctttcggca
    10861 tttactccgg gtatctgtat aacttttttc ttcgtac
//
LOCUS       DAOOQP010000100        10827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7602_length_10827_cov_5.612143, whole genome shotgun sequence.
ACCESSION   DAOOQP010000100 DAOOQP010000000
VERSION     DAOOQP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_10827_cov_5.612143"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..474
                     /locus_tag="PLK75_09860"
     CDS             94..474
                     /locus_tag="PLK75_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39592.1"
                     /translation="MKTSRKRNFIFVLFATVLLALIVGGMLLGSERYVVYNTSPCDIF
                     VVYKTYDGMNDSVCIEATKWKLIGKRMVWYKKYDKKNKCSVNQYFTEVKIYVGNKTGF
                     INALDSNWICASQPRLNEFTLTIK"
     gene            762..2186
                     /gene="dnaA"
                     /locus_tag="PLK75_09865"
     CDS             762..2186
                     /gene="dnaA"
                     /locus_tag="PLK75_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOY39593.1"
                     /translation="MAKRYDEAWNECLQIIRDNVPKVSYRTWFEPIIPLALDGNVLTI
                     QVPSTFFYEFLEEQYLDLLSKTLFKVIGPGAKLEYHIVMDNSDNAKPYTVKIPAIENT
                     KMVNKPVSVPLDPDAHSIKNPFVIPGIKKLQVDPQLNPDYNFNNFVEGECNRLARSAG
                     YAVAQNPGGTAFNPLFIYGSTGLGKTHLAQAIGIEVKNRFPEKIVLYVDANKFMTQYA
                     ESTRNNNRNDFIHFYQMMDVLIVDDVQYFTKKEGTQEVFFHIFNHLHQQGKQIILTSD
                     KPPVELNGMEQRLLSRFKWGLSADLQEPDFETRINILKQKTYKDGIIISEEILEYISS
                     HITSNVRELQGALISLLAQSTLNRKEITLELARDMIDKLVKSTKREISIDYIQKVVCN
                     YFNMPVESISSQTRKREIVQARQIAMFFAKNMTKSSLAAIGSLIGGKDHATVLHACKT
                     VNNLMETDKKFKLDVEEIEKRLKM"
     gene            2246..3106
                     /locus_tag="PLK75_09870"
     CDS             2246..3106
                     /locus_tag="PLK75_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOY39594.1"
                     /translation="MIWLLLAILSSTIILILFKLLKRYNIPTLNVIIVNYLTASVLGF
                     LLTQRAPASEISLSTWFLPAVGIAVIFIVSFFLIAYSSQNIGISVTSVAAKMSVIVPV
                     AFSIIYYQEPVNTLKIAGMVLALFAVFISVYRKEREKFVAKSMLIPVAIFLLMGTCDS
                     LIKFAQGAFVPDDVAPVFSASIFAVAFVLGLVYIVTKPSQFTEFRKLPVWWAGILLGA
                     VNFGSVYFIILALNSRFVDGSIVFGVVNIGVVGLSVIIGKFAFAEKLSALNIAGVILA
                     VTSIVLLMLS"
     gene            3112..3720
                     /locus_tag="PLK75_09875"
     CDS             3112..3720
                     /locus_tag="PLK75_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOY39595.1"
                     /translation="MDKDAYHSIREKTQGYFRDRGSKFLSFAFPVSCDEDVKNILNQL
                     RKEYYDANHHVYAYRLGVDENNYRSSDDGEPANSSGKPVLGQILSYGLSDVLVVVIRY
                     FGGVKLGVPGLIHAYRTAADDALKSADIIEKLVQRTICVEFPYSLMNQVLRLVEEEHA
                     EVFSRDFTEDCRLSFRVRKSHYKDVSIKLNQIFGVKIISDED"
     gene            3710..4270
                     /locus_tag="PLK75_09880"
     CDS             3710..4270
                     /locus_tag="PLK75_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY39596.1"
                     /translation="MKIKSALASFFLKILGWKPTEFPDVGNCVIAVAPHTSMWDFLWG
                     KLYILSKRRESCVLIKKEIFKFPLGWLLKMAGGVPVDRMSPQSLAKQLPKLFDEKPGL
                     AIAITPEGTRKKTNHWKKGFIFVAKAAQVPIVIGFINYKTKELGVLGVMDYNGDEDEI
                     MNNFKRKFIGMEGKHPEKFITGIENE"
     gene            4263..5036
                     /locus_tag="PLK75_09885"
     CDS             4263..5036
                     /locus_tag="PLK75_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOY39597.1"
                     /translation="MNELTISLVQTDLVWENPEANLNQLNTMLDSIRRTDIIILPELF
                     TTGFTMRCNGFAETMNGFTIHWMKEVATSKNAAVCGSIIIQEGTCFYNRFLFVTPDNV
                     YSYDKHHLFSIASEDKFYSKGSQRVIINYRNWRIMPLICYDIRFPVWSRNRNDYDMLI
                     YVASWPASRRKIWDALVRARALENQCVVAAVNRVGVDGAYIYYNGGTQLIDARGDTIA
                     MARDNHEEIFTTTLDKQKLDSFREKFPVWKDADPYYFNV"
     gene            complement(5026..6150)
                     /gene="mqnE"
                     /locus_tag="PLK75_09890"
     CDS             complement(5026..6150)
                     /gene="mqnE"
                     /locus_tag="PLK75_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOY39598.1"
                     /translation="MDKLNIEPREKQVSDGIRKITDKIKLSQRLNRNDALFLYEHFPL
                     GLLGELAFEWKRKISGDSVYFNRNIHIEITNICVNRCRFCSYYRDATSRDSWSLSMQD
                     VLEIIREKYIEGITEVHIVGGLNANYYLNFYTALFEQISALYPDLHIKAFTAVEIEHL
                     ARTSGRSIDSCIQSLKNAGLDSVAGGGAEIFNKAVRMRICEKKISAETWLSIHKELHK
                     NNIPSNCTMLYGHIESYSDRVNHLMRLRKLQDETQGFNCFIPLKFKNSNNKLSSVPES
                     NLAEDLRNIAVCRLVLDNIPHIKAYWPMLGKETARLCLHFGADDIDGTIHDTTKIYSM
                     AGSVEKRPVFDAWDINNAIRSEGFQAIERDSLYNKISPKR"
     gene            6238..7989
                     /locus_tag="PLK75_09895"
     CDS             6238..7989
                     /locus_tag="PLK75_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39599.1"
                     /translation="MPVRILSKYKHSITCLVAGVFVILLFRVINYTATEGFNPTDDGV
                     VLAQSYRLLQGEIPHLDFISIRPVFSGVMHSIHFFSPLPLLDSARWFVVLQFFIIAMA
                     VTSIVITLSGRSFSVITVFFLQLFLFTAEILNYNLFPWTTLDAVFLSTIGMWIYLAGY
                     NKNHFLKNALAVLLFTLAAISRQNFLLPAVFVSFLVFRRYFWEQRIIKGLLVIGIGWL
                     PVTLYFAMLVAYSALPEFGTQLSGRTELLQTGVLQYVKQWFYSPIMFMHLLIYVLVAM
                     HIIWGKRKQFFGNVIRKYYQGFALIAGVWIVFMLVLHFSRTETNIIMLPFELFWLLID
                     LLLVLWVVRKPNETLLSFIIITLLIAYTSSISLGDNSPVFAVGMLAGASLLLIHNMSD
                     DNGLFYGNRKLFRFAFISVSILIFVLGVFSQKSVNYRDRGAKQLVYELNNVRPAFGSV
                     RTNAITGAYYKELGNIFDTLPGAINHCVVLPHDAMFYPVFETRNPLPLDWMQPAEFVG
                     NEQRVIESVQQLIQKPGMYFIIDKYDIKKLYIRKVPLFRNDFPYVNLIEKSCQKMDSK
                     SLYFEIYKSRSAALSQR"
     gene            complement(7975..9381)
                     /gene="rlmD"
                     /locus_tag="PLK75_09900"
     CDS             complement(7975..9381)
                     /gene="rlmD"
                     /locus_tag="PLK75_09900"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOY39600.1"
                     /translation="MNKKKNLEILEEITITDIADEGMAVGRHNNMVVFVKNAVPGDIV
                     NVQLTRVKSHYREGVVHELVKASDYRVQPFCKHFGTCGGCKWQHLSYDKQLEFKQSQV
                     TNQLKRIGGLELPEPEPVIGAEKTTYYRNKLEYTFSDRKWFEPDEPIYEQGDKAAYAL
                     GFHAPFLFDKVVDIKQCYLQADPGNNIRNFVRDYALSNDLTYYSIRRQTGLLRNLIIR
                     NTLDGQFMVLLSIAENEPETAKKLLGALHKKFPEIKSLHFVINSTKNDTLATIEAVHV
                     FGDKSIAETMGSLKFAIGPKSFFQTNSSQAFRLYQKILEYASPDPNDVIFDLYTGTGT
                     IANFVAPHCSQVIGIESIAEAITDARTNAAINGISNAQFFTGDVKDVMNNDFLQQHKK
                     PSIIIVDPPRAGLHPQVTETILGVLPQKIVYVSCNAATQARDLKMMQPYYKIDSLQAV
                     DMFPHTQHIENLAKLSLR"
     gene            complement(9760..10548)
                     /locus_tag="PLK75_09905"
     CDS             complement(9760..10548)
                     /locus_tag="PLK75_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOY39601.1"
                     /translation="MPKSYKNFYKQNGVCPFCNENIPKVFDEFAIQSIHDRTLTIWEC
                     DCGYWELLNRYLIVEEVNYEDTYTNNWGFKDEFTTDVKIFTAIKNLEDRKKEKAIKQL
                     INEIQNNKNKMYSINPQKLEILAQYVFSAYFNCEVEHVGKSHDGGIDLLVVNSDKPIL
                     VQVKRRSRNDVVESVSTIRDFLGAMYINNNYNGIVVSTAKRFSKDSKKIITSLLEENR
                     LEYFEIFDFNRFVSILDVVNDTNKKNWIQYVESFKGLEKGTGSR"
BASE COUNT         3070 a         2043 c         2222 g         3492 t
ORIGIN      
        1 aaacacgcaa atacttttaa tcatactgtt taaaataaat acttaggtaa agttaaaaaa
       61 ttacacgcat tttactattt tcgaacaaaa aacatgaaga cttcacgaaa acgtaatttc
      121 atatttgttt tatttgcaac ggtgttattg gctttgattg tcggaggtat gcttttgggt
      181 tctgaaaggt atgtggttta taatactagc ccatgtgata tttttgtggt ttacaaaaca
      241 tatgatggaa tgaacgattc tgtttgtatt gaggcaacaa aatggaaact tattggtaaa
      301 agaatggtgt ggtataaaaa atatgataaa aaaaacaaat gtagtgtaaa tcagtacttt
      361 actgaggtaa aaatatacgt tggaaataaa acggggttta ttaatgcttt ggattcaaac
      421 tggatatgtg cctcacagcc aaggttaaac gaattcacgc tgactatcaa ataaaaaaaa
      481 taaaaaaaaa agtaaaatgt agtgttggta ttgcagaaaa ttcctatttt tatcaatctg
      541 tgaggaaagg caattagaaa tttgaggatt atattgaatt gaatgtaact aattgatttt
      601 aaagtagtta tttgttattt attgtaattg aaaataaaaa gtttgaagtc aaatatttta
      661 attaaacaag agaaaaaaaa ttttttttaa aaaaagattg ttgaatattt gctttttcat
      721 atggcgattg ttgtgttaat taataataat taagtaggct catggcgaag agatacgatg
      781 aagcatggaa tgagtgcttg caaattatca gggataacgt accgaaagta agttatcgca
      841 catggtttga gcctattatt ccgttggccc ttgatggcaa tgttcttacc atacaggtgc
      901 caagtacttt cttttatgag ttcctcgaag agcaatatct cgatttactc agcaaaaccc
      961 tgtttaaagt gattggtccg ggagcaaagc ttgaatatca cattgtgatg gacaactccg
     1021 ataatgccaa accttatact gttaaaatcc cggctatcga gaataccaaa atggtgaata
     1081 agcctgtatc ggtgcctctc gacccggatg cccattctat taaaaacccg tttgtaattc
     1141 caggaataaa aaagttgcag gttgaccctc aactcaaccc ggattacaat ttcaataatt
     1201 ttgtagaagg agaatgtaat aggctggccc gttctgccgg atatgccgtg gctcagaacc
     1261 ccggaggtac tgcatttaac cccttgttta tttatggctc aacaggattg gggaaaaccc
     1321 atctggctca ggccattggc attgaagtga aaaaccgttt ccccgagaaa atagtgcttt
     1381 atgttgatgc gaataagttc atgactcaat atgcagagtc aactcgcaac aataaccgta
     1441 acgattttat ccacttctat cagatgatgg atgtgcttat cgttgatgat gtgcagtatt
     1501 ttaccaagaa agaagggaca caggaagttt tcttccatat ttttaatcac ctacatcagc
     1561 aggggaaaca aattatactt acatccgata agcctccggt tgaattgaac ggtatggagc
     1621 aaaggttgct ttcaaggttt aaatggggac tttctgccga tttgcaggaa cccgatttcg
     1681 aaacacgaat caacattctt aagcaaaaaa cttataaaga tggaattatc atttcggaag
     1741 aaattctgga atatatttca tcgcacatta caagcaatgt ccgcgaattg cagggagccc
     1801 tcatttcgtt gcttgcacag tcaacactga accgtaagga aattactctt gagcttgccc
     1861 gcgatatgat cgacaaactc gtgaagagta caaaacgcga gatatcaatt gattacattc
     1921 agaaagttgt atgcaactat ttcaatatgc ctgttgaatc aataagcagc cagacacgta
     1981 agcgcgaaat cgttcaggca aggcagattg ctatgttttt tgcaaaaaac atgacaaaat
     2041 catcgctagc agcaatcgga tcgcttattg gcggaaaaga tcacgctact gtgctccatg
     2101 cctgcaaaac agtaaataac ctgatggaaa ccgataaaaa gtttaaattg gatgtggagg
     2161 aaattgaaaa acgattgaaa atgtaaaccg taatatcaaa agggctgaaa acagcccttt
     2221 tttattgatc taatcttatt tgcacatgat ttggctgctg ctggcaattc tttcatcaac
     2281 tattattctg atactgttca aactcctcaa gaggtataat atacccacat tgaatgttat
     2341 tattgtaaac taccttaccg ccagtgtgtt gggttttctt cttacacaga gagcgccggc
     2401 ttccgaaatt tcactgagca catggttttt acctgctgtt ggaatagcag tgatttttat
     2461 agtgtcgttc ttccttattg catacagttc acaaaatatt ggaatttcgg tcacttcggt
     2521 tgctgcaaaa atgtcggtaa ttgttccggt agccttttct ataatctatt accaggagcc
     2581 tgtgaataca ttgaaaattg caggaatggt tctggcgctt tttgccgtat ttatttcggt
     2641 ttaccgcaaa gagagagaaa aatttgttgc taaatcaatg cttattccgg tagcaatttt
     2701 tctactaatg ggtacatgcg actctttaat taaatttgca cagggggctt ttgttcccga
     2761 tgatgtggca cctgtttttt ctgcgtcaat ttttgcagtt gcctttgttc ttggcttggt
     2821 ttatattgta acgaaaccat ctcaattcac cgaattcagg aaattgccgg tgtggtgggc
     2881 tggtatttta ctgggtgccg ttaattttgg ctcggtttat tttattatac ttgcgctgaa
     2941 cagccggttt gtagatggaa gcattgtgtt tggtgtggta aatatcgggg tagtaggttt
     3001 atcggttatt atcggtaaat ttgctttcgc agagaagctt tcggcgttaa atattgccgg
     3061 ggtgattctt gcagttacgt caattgtatt attaatgctg agttaaaaca gatggataag
     3121 gatgcatacc atagtatccg tgaaaaaaca cagggttatt tccgcgacag aggaagtaag
     3181 ttcctgtcat ttgcattccc tgtttcctgt gacgaagatg taaagaacat cctgaatcaa
     3241 ctgagaaaag agtattacga cgccaatcac catgtgtatg cctatcgtct cggggttgat
     3301 gaaaacaact atcgctcgag cgacgacgga gaacctgcca acagtagcgg taaaccggtg
     3361 ctgggccaga tactgagtta tggtttaagc gatgttctgg tggttgttat tcgttatttc
     3421 ggaggtgtga aactgggggt tcccggtctg atacatgcct atcgcaccgc agcagatgat
     3481 gcccttaaaa gcgcagatat catcgagaag ctggtacaaa gaaccatatg tgttgaattc
     3541 ccgtactcac tcatgaatca ggtgctgcga ttggttgaag aagagcatgc cgaagtgttt
     3601 tcgcgcgatt ttaccgaaga ttgccgcctt agcttcaggg ttcgcaaaag ccattacaaa
     3661 gatgtaagta taaaactaaa tcaaattttt ggagttaaaa ttatcagtga tgaagattaa
     3721 aagtgctctg gcatcattct tccttaaaat tttgggatgg aagcccacag agtttccgga
     3781 tgtagggaat tgtgtaattg ctgtggcccc acacaccagt atgtgggatt tcctgtgggg
     3841 aaaattatac atactttcaa aacgacggga gtcatgcgta ttaataaaaa aagagatttt
     3901 taaatttcct ctgggttggc ttctgaagat ggcaggaggg gtacctgttg acagaatgtc
     3961 accgcaaagc cttgcgaagc aattgcctaa attgtttgat gaaaaaccgg gtctggcaat
     4021 tgcgattaca cccgaaggaa cacgaaaaaa aacaaaccat tggaaaaaag gttttatctt
     4081 tgtagctaaa gcagcacagg tacctattgt tattggtttt attaattata aaacaaaaga
     4141 gctgggagtg cttggtgtaa tggattacaa cggagatgaa gatgagatta tgaacaattt
     4201 taaacgtaaa tttataggaa tggaagggaa acaccctgaa aaatttataa ccggcatcga
     4261 aaatgaatga attaacaatt agcctagtac aaacagattt ggtttgggaa aatcctgaag
     4321 ccaatctgaa ccagcttaat acaatgctgg attctatacg cagaaccgat ataattattc
     4381 ttccggaatt gtttacaaca ggatttacta tgcgttgcaa cggttttgca gaaaccatga
     4441 atggttttac tatacattgg atgaaagaag tggcaaccag caagaacgca gccgtttgcg
     4501 gaagcattat tatccaggaa ggtacatgct tttataatcg gtttcttttt gtaactcccg
     4561 ataatgttta ctcttacgac aaacaccact tgttcagcat tgcctctgaa gataaatttt
     4621 acagtaaagg ctcacaacgg gtcattataa actatcgcaa ctggaggata atgcccctga
     4681 tttgctacga tatacgcttc cctgtgtgga gccgcaacag gaacgattac gatatgctta
     4741 tttatgtggc tagctggcct gcaagccgcc gtaaaatatg ggatgctctt gtaagggcaa
     4801 gagctttaga aaatcaatgt gttgtagctg ccgtgaaccg agtgggtgtt gatggcgctt
     4861 acatatacta caatggtgga actcagctta tcgatgcacg aggcgatacc attgcaatgg
     4921 cgcgcgataa ccatgaagaa atttttacta ccaccctcga caagcaaaag cttgatagtt
     4981 tccgtgaaaa atttccggta tggaaagatg ctgacccata ctactttaac gtttaggaga
     5041 aattttatta tataaggaat ctctttctat tgcctgaaac ccttcggagc gaatggcatt
     5101 attgatgtcc catgcatcga aaacagggcg cttctcaact gagccggcca tactgtatat
     5161 ttttgttgta tcgtgaatcg tgccgtcgat atcgtcggcc ccaaaatgca ggcacagtct
     5221 ggctgtttct tttccaagca ttggccaata tgcttttata tgtgggatgt tgtcaagtac
     5281 aagcctacaa acagcaatat ttcgtaaatc ttctgccaga ttgctttcag gaaccgagct
     5341 gagtttattg tttgagtttt taaattttaa cggtataaaa caattgaacc cttgcgtttc
     5401 atcctgaagt tttcgcaacc gcatcaaatg gttaacccgg tcggaataac tctcaatgtg
     5461 accataaagc atggtgcaat tgcttggaat gttgtttttg tgaagctctt tgtggatact
     5521 caaccatgtt tcggcagaga tttttttttc gcagatgcgc attcttacag ctttgttaaa
     5581 aatttcggca cctcctcccg ctaccgaatc taagcctgca ttttttaatg actgaataca
     5641 agaatcaatg ctccttccac ttgttctggc aaggtgctct atctcaacag cggtaaaagc
     5701 tttgatatgc aaatcgggat ataacgcaga tatttgttca aagagtgcag tatagaaatt
     5761 caggtagtag ttcgcattca aaccaccaac aatatgcact tctgtgatgc cttctatgta
     5821 cttttcgcgt attatctcaa gcacatcctg catgctcagc gaccatgaat cgcgagaggt
     5881 ggcatcgcga taatacgaac agaacctgca acgattcaca cagatatttg tgatttcgat
     5941 gtgtatattc cggttgaaat acaccgaatc tccggaaatt ttacgtttcc attcaaaagc
     6001 cagttctccc agtaagccta aggggaaatg ctcataaagg aataacgcat cgtttcggtt
     6061 aagccgctgc gacagtttga ttttatcggt aatttttcta atgccatcag aaacctgttt
     6121 ttcccgaggc tcaatgttca gtttatccat atcacaaaag tacctaattg ccccatgcaa
     6181 agtacaaatt tataattttc tgccttacca atcaaatgat gtctatcttt gtaaactatg
     6241 cctgttagaa tattgagcaa atataagcac agtataacct gtctggttgc aggtgtattt
     6301 gtgattttac tgtttcgggt tattaattat accgccaccg agggtttcaa ccccaccgat
     6361 gacggagttg tccttgctca gtcgtatcgt ttgttgcaag gcgaaatacc gcatctcgat
     6421 tttatctcaa ttcgaccggt gttcagtggt gttatgcaca gtattcactt tttttcgccg
     6481 ctgcccttgc tcgacagtgc gcggtggttt gtggtattac agttttttat cattgcaatg
     6541 gcagttacaa gcattgtaat tacactgagt ggtcgcagct tttccgtaat tacagttttc
     6601 tttttgcaat tatttctgtt tactgccgaa attctgaact ataatctatt cccatggaca
     6661 acccttgatg cggttttcct ttctaccatc ggaatgtgga tttaccttgc aggctacaat
     6721 aaaaaccact ttctgaaaaa tgcgcttgca gtattattat ttacactggc ggctatctca
     6781 aggcaaaact ttctgcttcc ggctgttttt gttagcttcc ttgtgtttag gcgttacttc
     6841 tgggagcaac gtattattaa aggcctgctt gtaataggca tcggctggtt accggtaacc
     6901 ctttattttg ctatgcttgt tgcttattct gctcttccgg agtttggtac gcagctttcc
     6961 ggacggacag agttattgca gaccggagta ctgcaatatg tgaagcaatg gttttattcc
     7021 ccgattatgt ttatgcattt gctcatatat gttctggttg cgatgcatat aatttgggga
     7081 aaaagaaagc agtttttcgg gaacgtcata cgcaaatatt atcagggctt tgcgcttatt
     7141 gccggtgtat ggatagtatt catgcttgtg ttgcactttt cccggacaga gaccaatatc
     7201 attatgcttc cttttgagct gttttggtta ttaatcgatt tattgttggt gttgtgggtg
     7261 gtgcggaaac caaacgaaac ccttcttagc ttcattatta ttaccttgtt gatagcttat
     7321 acatcttcta tttcgctcgg cgacaacagc cctgtgtttg ctgtgggcat gctggccggt
     7381 gcttctttgt tgctaattca taacatgagc gatgataacg gattgtttta cggaaatcgg
     7441 aagctttttc gttttgcttt tatttctgta tcaatattaa tatttgtttt gggtgtattt
     7501 tctcagaaaa gtgtgaatta tcgcgacagg ggggcaaaac agcttgttta cgaactgaac
     7561 aatgtacgcc cggcttttgg ttcggttcgc accaatgcca taaccggtgc atattataaa
     7621 gagcttggga atatttttga tacccttccc ggagcaataa atcattgtgt tgtgttgccg
     7681 catgatgcca tgttttaccc tgtattcgaa acgcgtaacc cactgcctct cgactggatg
     7741 caaccggctg aatttgtcgg aaatgagcaa cgggtaattg agtcggtgca gcaactcatt
     7801 caaaaaccgg gaatgtactt tattatcgat aaatacgaca ttaaaaaatt gtacatccgc
     7861 aaggtcccat tattccgtaa cgattttcct tacgtgaatc tcattgagaa atcctgccaa
     7921 aaaatggact caaagagcct ctacttcgaa atatataaga gcaggtctgc agccttatcg
     7981 caacgataat tttgccagat tttcgatatg ctgggtatgc gggaacatat ctaccgcctg
     8041 aagtgaatca attttataat acggttgcat catctttaaa tcccttgcct gcgttgctgc
     8101 attgcaactg acataaacaa ttttttgcgg taacacaccg agaatggttt cggtaacctg
     8161 aggatgcaat cctgcgcgag gcggatcaac aattattatt gatggttttt tatgttgttg
     8221 taaaaaatcg ttgttcatca catcttttac atcgcctgtg aagaattgag catttgaaat
     8281 gccgtttata gctgcatttg ttctcgcatc ggttattgct tcggcaatac tttcgattcc
     8341 tattacctgg ctgcaatgcg gcgcgacaaa attggcaatg gttccggttc cggtgtacag
     8401 gtcgaaaatc acatcgttcg ggtcgggtga ggcatactca agtatttttt gatacaacct
     8461 gaatgcctga gagctgttgg tctgaaaaaa cgacttcgga ccaatggcaa atttcaacga
     8521 tcccattgtt tcggcaatag atttgtcacc aaaaacgtgt actgcctcaa tcgttgctaa
     8581 tgtgtcgttc ttcgtgctgt taattacaaa gtgcaacgat ttgatttcgg gaaatttttt
     8641 gtgcagtgca cccagcaact tcttcgcggt ctctggttca ttctctgcaa tactcagcaa
     8701 taccataaac tgcccgtcga gtgtgttacg gattatcaga ttacgtagca aacctgtctg
     8761 cctgcgaatg ctgtaatatg tcaaatcgtt gctgagagcg taatctctta caaagttccg
     8821 gatgttgttt ccggggtcag cctgaagata gcattgtttt atatccacta ctttatcgaa
     8881 gagaaaagga gcgtgaaagc caagtgcata ggcagctttg tcgccctgtt catagatcgg
     8941 ttcatcgggt tcaaaccatt tgcgatcaga aaaggtgtat tcaagtttgt tgcgataata
     9001 tgtagttttt tcagccccaa taacgggttc cggttcgggt aattcaagtc cgccgatacg
     9061 ttttagctgg ttggtaacct gactttgttt aaattcaagt tgtttgtcgt agctcaggtg
     9121 ctgccacttg catcctccac aagtgccgaa atgcttgcaa aacggttgca ccctgtaatc
     9181 tgaagctttt acaagttcat gaaccacccc ttcgcgataa tggctcttca ctcgtgtaag
     9241 ttgcacattc acgatatcgc ccggaacagc attcttaaca aacaccacca tattgttgtg
     9301 gcgccctact gccatacctt cgtcggcaat gtcggtaatg gttatttctt ctaggatttc
     9361 aaggtttttc ttcttgttca tcgtattatt ttattcaacc gtaaaggtaa taaaccgcta
     9421 aggttttgca actgttgtat gtgtcgggtg tagcgtaaag caatatttcg ttgtgtgaga
     9481 ggtgttttta gatgtattcg aaggactctc cccgtcagtg gttgatgagc ttgtcgaatc
     9541 atactggcgg ggctgtctac tcgattacag gtgttgattt tttgatttat gaaatcgttt
     9601 ttcggattat gctgactcct ccacagtttg taaagcgata ccgatagtgg ctatttttta
     9661 ttgaatcccc ataatatcgt acccaagcct tcaatgttga ccgcgcgggg tttcgttcaa
     9721 catggcttca tgtttggcaa gccacacgaa gcctaattat tagcggctgc ccgttccttt
     9781 ctccagtcct ttaaaacttt ccacatactg aatccagttt ttcttatttg tgtcatttac
     9841 aacatcgaga attgatacaa atcggttgaa atcaaaaatc tcaaaatact ctagtcgatt
     9901 ttcttctaat aaagaagtga taatcttttt agaatctttt gaaaatcgct ttgcagtaga
     9961 aactactata ccattataat tattatttat atacattgca cctaaaaagt ctctgatagt
    10021 gctaacactt tctactacat catttctact tcttctcttt acttggacta aaattggttt
    10081 gtcggaatta acaacaagta aatcaatgcc cccatcgtgt gattttccga catgttctac
    10141 ttcgcaattg aaatatgcag aaaatacata ttgggctaaa atctctaatt tttgaggatt
    10201 aatggaatac attttattct tgttgttttg tatttcattt attaattgct ttattgcttt
    10261 ttcttttttt ctatcttcta aattttttat tgctgtaaat attttgacat cagttgtgaa
    10321 ttcatcttta aaaccccaat tgtttgtata tgtgtcttcg taattaactt cctcgacaat
    10381 aaggtagcgg tttagtagtt cccaatagcc acaatcgcat tcccaaatag ttaaagttct
    10441 atcatgtatt gattgaatag caaattcgtc aaatacttta ggtatatttt cattgcaaaa
    10501 aggacaaaca ccattttgtt tataaaagtt tttgtaactt ttaggcattt catatatttt
    10561 ggttgtacga ttcaacgaat ctttgtcata tgaaaatctg tcaataatta attcctggaa
    10621 atttagattt attttattca ttttattact aactattttg aatacacatt ctaagggttc
    10681 tgactaacgg tcacgcaaat ctctgatttt catatgtgac tattttggtt ggagctccgt
    10741 aactcaatgt ttttcatttt cagggttgcc gctaacggat ggcaataaga aacgtagggc
    10801 atttcgaagc ttctacttat taagtta
//
LOCUS       DAOOQP010000101        10621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7786_length_10621_cov_6.799735, whole genome shotgun sequence.
ACCESSION   DAOOQP010000101 DAOOQP010000000
VERSION     DAOOQP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7786_length_10621_cov_6.799735"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="PLK75_09910"
     CDS             complement(<1..1691)
                     /locus_tag="PLK75_09910"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOY39602.1"
                     /translation="MKKYFIVFSSILLLATFSANAQIIYVKHDATGTNTGNSWENAFT
                     SLQSALGIAVSGDQIWVARGTYKPSYDYGLGGGARYYHFRMIDGVAIYGGFAGTETLV
                     SQRTDFGEGGNNETILSADLNGDDILVGDGNLISISNNSENCYHVFMHPSAMTPLLTN
                     TAILDGFTITGGNADGTVAPYTSGGGILCDKNSPTLNQLIIKYNFAKGGGGITISGDE
                     INPGNNNTKLTNSVIKNNLSSGSGAGVNMANCSADAEVINCTISGNKTTLNPGSFNQG
                     GAGVRIYHRAKFINCIIENNWAPDCTAGGGGAFIDYGTFWGSQGIFFINCTIINNYAK
                     NGGGTNYVITGGEFRNCILWGNTDQNGVSNYIGSSFAYCNTYPLPSGTGNISIYPDFV
                     DPLSKDFRICGNSICVNSGNNAYNSQLSDIRGQVRIQNTTIDMGAYEFTSGVDPIRYY
                     VKHDASGLNNGTTWENAFTSLQSALNVATAISEIWVAKGTYKPSYDYGIGGGLRFNHF
                     RMINGVSIYGGFAGNEISINQRSDFGEGEVNETILSADINDDDVVAGSGSNLSIT"
     gene            complement(1950..4049)
                     /gene="recG"
                     /locus_tag="PLK75_09915"
     CDS             complement(1950..4049)
                     /gene="recG"
                     /locus_tag="PLK75_09915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOY39603.1"
                     /translation="MGLDSKIQFLKGVGPKTAELLKSEIGIESFEDMLYYFPYRYIDR
                     SKFYNISEIDENLPYIQITGYFSSFENIGARGKTRVTGVFNDETGSIEIIWFKGTKWI
                     TSTYKPDKKYILFGKPVRYGSKINMVHPEVEDLETWSRKIGKEVSPQYSTTEKMKNHG
                     LSSRTLQNIEDRLLKECAEQIYETLPAYIISKYQLMPLKSAIKEMHFPGSIEQLNKAQ
                     YRLKFEELLYIQLRILRLKLMRKVSSTGIVFNRDRDSLVAECFRNLPFKLTNAQIKVL
                     RDIRHHVESGKQMNVLLQGDVGSGKTLVALLSMLMAVDNGFQACLMVPTEILAQQHYI
                     SFSKYLAKLDIAVALLTGSTKKAERKTIHEGLTNGSLKILIGTHALIEDTVNFTNLGY
                     VVIDEQHRFGVAQRAALWAKSVNPPHILVMTATPIPRTLAMTLYGDLDVCVIDEMPPG
                     RKPIITRHVTDNSRLKVFGWMEKLIALGKQIYVVYPLIEESENFDYKTLEEGVEGISR
                     AFPLPKYTISVVHGRMKSNDKEFSMNMFITGKANILIATTVIEVGVDVPNAAMIVIES
                     AERFGLSQLHQLRGRVGRGQDQSYCILMTENKISENALNRIQAMVDTNDGFTLAETDM
                     RLRGPGDLEGTRQSGFDINLRVANLAKDGQILLYVRQIAESILDKDPDLDSPENSLLK
                     KQLNKMKDSSTDWRQIS"
     gene            4153..4926
                     /locus_tag="PLK75_09920"
     CDS             4153..4926
                     /locus_tag="PLK75_09920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY39604.1"
                     /translation="MIDLRWIICSRKPGHFADERLKSMFSFVFGKTYGYYVFSEIENQ
                     RKILLETHTEIEVIDKGAGSRVFKGNKRSIQRIARYCLTEQEDAQILFRLGAWAGSNH
                     ILELGTSLGVTTAYLATTPCNLELISIDACSNTIKEAKKLLSELSVENVTLLNSDFSL
                     AIENLIKENRKFDFIYFDGDHNGLRMRNYYEGVRLNLVGKRYVMVFDDISWSRDMNTF
                     WKYIARNSDSCIISTGTTGYVIHGFDLPCGYFMLKLSKT"
     gene            4979..5674
                     /locus_tag="PLK75_09925"
     CDS             4979..5674
                     /locus_tag="PLK75_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY39605.1"
                     /translation="MSNNTVIQLKDASKDYYVGINVVNALKSISLYIEKNEFVAIMGP
                     SGSGKSTLMNIIGCLDTLTKGSYILNGSDVSDLTDNSLATIRNKEIGFVFQTFNLLPR
                     YSALDNVMLPLVYAGVPSKKRKEIAEEVLLNVGLQDRMHHKPNELSGGQRQRVAVARA
                     LVNKPSIILADEPTGNLDTKTSEEIMELIEQIHNKGNTIVLVTHEEDIALHAKRIVRL
                     RDGLIESDALNNN"
     gene            5797..6354
                     /locus_tag="PLK75_09930"
     CDS             5797..6354
                     /locus_tag="PLK75_09930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOY39606.1"
                     /translation="MKIYTKTGDDGTTGLIGGVRVSKADIRVEAYGTVDEANSWLGLI
                     RAVCNYEEINQELICIQRNLFTICAHLASDYKRVPQSAPAIPVLDPEFSKFLEKSIDK
                     YNESIPALENFIIPGGSQLSASCHLARAVCRRAERRVTALAGTAKVDAEILVFLNRLS
                     DYLFVLARYFSFKQHVDEIFWKNDL"
     gene            6424..6645
                     /locus_tag="PLK75_09935"
     CDS             6424..6645
                     /locus_tag="PLK75_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOY39607.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPLEVIENLNEIE
                     DEGEIYESIEDIWPDYPSKDDFFFNEDEY"
     gene            6835..7863
                     /locus_tag="PLK75_09940"
     CDS             6835..7863
                     /locus_tag="PLK75_09940"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY39608.1"
                     /translation="MKKSHLIPILFSCATYSISFAQINVVPVTDPVQAAASFVFDGVS
                     VTNVVYTGDENAFGSFSNATGIVPGLESGLVLSTGSINPEGSAPVGAPVSSFANTIND
                     APGHYLLNCLISSDTYDAAVFEFDLVPIGNILEFQYVFASEEYPEFAGSSFNDVFGFF
                     ICGPNPNGGNYNNVNIAIIPGTTSPVAINNVNGNVNSSFYIDNEALNNPDFVFDGSTV
                     LLTAQINVIAGNTYHLVMAIADVGDMCYDSAIFLKASSMKSYIFSGEQNPATIKPMVF
                     YDAVSKTICIQYVDGGPIQLQLFNLQGAMVLSGMNESLSTEGLKKGVYLAKISFGENV
                     AVEKILID"
     gene            complement(7869..8585)
                     /locus_tag="PLK75_09945"
     CDS             complement(7869..8585)
                     /locus_tag="PLK75_09945"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOY39609.1"
                     /translation="MSELTVYQQIMETKEHGKHLLAMLIDPDKFSEQNFREFVKSSDF
                     SAVDLLFAGGSFLSTGTNSCIEGIKKYTDKPVILFPGNACHISKNADAILLLSLISGR
                     NAEFLIGNHVTAAPFIKKSGIEAISTGYMLIGNGNDTSVEYISNTHPIPAAKTDIAVA
                     TALAGEMLGMKLIYLEAGSGARKSVPDEMVRAVSNACNSPLIVGGGINTQDQMERKFE
                     NGATIVVIGSAFEKGKSFKR"
     gene            complement(8622..9242)
                     /locus_tag="PLK75_09950"
     CDS             complement(8622..9242)
                     /locus_tag="PLK75_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOY39610.1"
                     /translation="MITDLNISGTRIVCWQITESADILTGSLPENIQKEAVKYTHIGR
                     REEFAASRLCLQHAGFKDPIYKDTWGKPFINNAHIGITHTDGFAAVIISKTMCVAIDA
                     EQPDVRLLRTAPRFLNNYESEYCGNDIEKIALIWCCKECIYKLYGHGYSDFKQSLEVK
                     ETDDGNFEGKIQGKDIVILRRLNLSGENMPVIVWCSQAPENIAIQD"
     gene            complement(9248..9820)
                     /gene="gldD"
                     /locus_tag="PLK75_09955"
     CDS             complement(9248..9820)
                     /gene="gldD"
                     /locus_tag="PLK75_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOY39611.1"
                     /translation="MKTSSILLSVAIIALLFASSCKKHYAPRPYGYFRIDFPEKDYKT
                     SDTTFPYSFEMPSYSFMQADTIKLNWANLVFPLNHAIIYLTYKELNNDFETHMEESRE
                     FVYKHTVKADAIEESVFVNEDEKVYGILYDIKGNAASSVQFFVTDSTRHFLRGSLYFD
                     CPPNKDSLDPVISFIRTDIVHIMETLKWAK"
     gene            complement(9807..>10621)
                     /locus_tag="PLK75_09960"
     CDS             complement(9807..>10621)
                     /locus_tag="PLK75_09960"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOY39612.1"
                     /translation="SRVNKRMQRRHKQNISIDDLSHAIELASDELNEEKEMLEGIINF
                     TNIEVKEIMKPRMDITAFEYSETFTKVLSVIVETGYSRIPVYKENPDNIVGVLYIKDL
                     LPYIHIEDKKSFKWQKLIRTHLIIPETKKINDLLKDFQSKKMHMAVVVDEYGGTCGIV
                     TMEDILEEIVGDINDESDTDGILFTQISENTWEFDGRTTIIDFCKTLDVDSNLFDAVR
                     GESDSLAGLILEMKGDIPHKNENINYSRFTFTIESVDKRRIKKIRVTIHENK"
BASE COUNT         3038 a         2136 c         2074 g         3373 t
ORIGIN      
        1 tttgtaatgg aaagattgct gccactacca gctaccacat catcgtcgtt aatatcagca
       61 ctgagaatag tttcgttgac ttccccttcg ccaaaatcac ttctttgatt aatacttatt
      121 tcatttccgg caaaaccgcc gtaaatagaa accccgttaa tcattcggaa atgattaaat
      181 cgaagccctc ctccaatgcc ataatcatac gaaggtttat aagttccttt ggcaacccaa
      241 atctcgctaa tagcggttgc gacattaagt gctgattgca acgatgtaaa tgcattttcc
      301 caagttgttc cattatttag tccgcttgcg tcatgtttta catagtatct tatcgggtcg
      361 actcctgacg taaattcata ggctcccatg tcgattgtag tattttgaat tcttacctga
      421 cccctgatat cggagagttg agagttatag gcattgttcc ctgagtttac acaaatcgaa
      481 ttgccacaaa ttctgaagtc cttggataat ggatcaacaa agtcaggata aatactaata
      541 tttccagttc ctgaggggag agggtaagta ttgcagtaag caaaactact gccgatatag
      601 tttgaaacgc cattttggtc ggtatttccc caaagtatgc agttgcggaa ttctccacca
      661 gtaataacat agtttgtccc tccgccgttt tttgcgtaat tgttgataat ggtgcagtta
      721 ataaagaaaa tcccctgtga accccaaaag gtgccgtaat cgataaaagc accgcccccg
      781 cctgctgtac agtccggtgc ccaattattc tcaataatgc aatttatgaa ttttgcccgg
      841 tgatagattc ttacccctgc tccaccttga ttgaaactac ccgggttgag tgtggttttg
      901 tttccgctga tagtacaatt tattacctca gcatctgcgc tgcagtttgc catatttaca
      961 cctgcacccg agcctgaaga aagattgttt tttattactg aattcgtaag cttcgtgttg
     1021 ttattacctg gatttatttc gtcccccgaa atggttatac cgcctcctcc ttttgcaaaa
     1081 ttatatttta ttattagttg gttaagggta ggggaattct tatcgcagag aattccgccc
     1141 ccactagtat aaggagcaac tgttccatcg gcattacctc cggttatggt aaatccatcg
     1201 agaattgctg tattggtgag cagaggtgtc attgcagaag ggtgcataaa tacatgatag
     1261 caattctcac tgttatttga aatactaatt aggtttccat cccctacgag aatatcgtca
     1321 ccgttcaggt ctgcgctgag aatagtttcg ttattgccgc cctctccgaa gtcggttcgc
     1381 tgcgaaacca atgtttcggt tccggcaaac ccgccgtaga tcgcaactcc gtcaatcatc
     1441 ctgaaatggt aataccgtgc gccgccaccc aatccataat catatgacgg tttgtaggtg
     1501 ccacgcgcaa cccaaatctg atcgccactt acagcaatgc caagagccga ttgaagtgat
     1561 gtaaatgcat tctcccagct gtttccagta ttggtgcctg tagcatcatg ttttacataa
     1621 attatctgag catttgcgct gaaagttgct agaagtaaga tgctggaaaa tacgatgaaa
     1681 tactttttca tattttagtt ttttggttga ccgtagaata taatacaaac caagttgcac
     1741 aatgggaaat atcctaataa caaaacagat atggtttccc tggttgaaca aggttagtat
     1801 atacaaatat atgaaataaa tttgtataag tcaatacttg ttatatttgg atgattacca
     1861 tcggtagtct catagcatgg gtacatttac acaaagcaaa tgaatatggt aaattatata
     1921 aaatatttac gtaaagtata tcagtattgt caacttattt gtcgccaatc agtagaagag
     1981 tctttcattt tgttgagttg tttttttaat agcgaattct ccggagaatc gagatccggg
     2041 tctttgtcga gaattgattc tgctatttgg cgaacatata gtaatatctg accatctttg
     2101 gctagatttg ccacccgaag gttgatatca aatccgcttt gacgtgttcc ttcaaggtcg
     2161 ccggggcctc ttagcctcat gtcggtttct gcaagtgtaa atccgtcatt tgtgtcaacc
     2221 atagcctgga tacggtttaa agcattttcc gatattttat tttcggtcat cagaatgcaa
     2281 tacgattgat cctgcccccg acccacacgc cccctcaact ggtgcaactg cgataatcca
     2341 aagcgttcag cgctttctat tacgatcatt gctgcattgg gaacatcaac acccacttcg
     2401 ataactgttg ttgcaattag aatattcgct ttacctgtaa taaacatatt catcgaaaac
     2461 tctttgtcat tagatttcat acgcccatgc acaacagaaa tagtgtattt gggaagaggg
     2521 aatgcgcgcg aaattccttc aactccttct tcaagggttt tataatcgaa gttttctgat
     2581 tcttcaatta atggatacac aacataaatc tgcttcccta aagcaataag cttctccatc
     2641 catccaaata ctttaagacg gctgttgtcg gtaacatggc gggtaataat tggctttcgg
     2701 cctgggggca tttcatcgat tacgcaaacg tcaagatcac cgtatagtgt cattgccaat
     2761 gtccttggaa ttggggtggc tgtcataacc agaatatgag gcgggttaac tgactttgcc
     2821 cataaagcag cgcgttgcgc aacaccaaaa cgatgttgct catcaatgac aacatagccg
     2881 agatttgtaa aattcacagt atcttcaatc aaggcatggg ttccgattaa aattttcagg
     2941 ctgccgttag ttaaaccttc atgaatagtt tttctctctg cttttttagt gctgccagta
     3001 agcagggcaa cggcaatgtc caattttgct aagtattttg aaaacgaaat ataatgctgt
     3061 tgtgccaata tttctgtggg gaccatcagg caagcctgaa atccgttatc aacagccata
     3121 agcatactta gcagtgctac cagtgttttt ccgctaccga catctccctg aagaagcaca
     3181 ttcatttgtt ttccactctc aacatgatgt cgtatatcgc gcagaacttt tatctgagca
     3241 ttggtaagtt tgaaaggcag gttccgaaaa cactccgcaa ccaatgaatc gcgatcacgg
     3301 ttgaagacaa ttcctgttga actgactttg cgcatcagtt tcaaccgcaa gatacgaagt
     3361 tgaatataca gaagttcttc aaattttaag cgatactggg ctttatttaa ttgttcaata
     3421 cttccgggaa aatgcatttc tttaattgcg ctttttagcg gcatgagctg atatttcgaa
     3481 ataatatatg cgggaagtgt ttcgtaaatc tgctctgcac attctttaag caaacggtct
     3541 tcaatatttt gtaatgtcct gctgctcagt ccatggtttt tcattttttc ggtagtactg
     3601 tactgcggac tgacctcttt acctatcttt cgtgaccatg tctctaagtc ttccacttcg
     3661 gggtgtacca tgtttatttt tgagccatag cgtacaggct taccgaataa aatatacttc
     3721 ttatcgggtt tataggtgct cgttatccat tttgtccctt tgaaccagat gatttcgatg
     3781 ctacccgttt cgtcgttgaa aacaccggtt actcgtgttt tccctcttgc gccaatattt
     3841 tcaaaactgc tgaaatatcc tgtgatttga atgtatggca gattctcatc aatctcgctg
     3901 atattgtaaa acttcgaacg gtcaatgtag cgatatggaa aataatacaa catatcctca
     3961 aagctttcaa tgccaatttc cgattttaac aactcggccg ttttggggcc tacccctttg
     4021 agaaattgta ttttactgtc aaggcccata gaggaatata aaaatatcga attcaatttg
     4081 cgtaaaatta tcaaaacttc atgaataatt atctaaattt accccaatga atagtataaa
     4141 aatgagtttc tgatgattga tttacggtgg ataatctgct caagaaaacc cggccatttt
     4201 gcagatgaac gcctgaaatc aatgttctcg tttgtatttg gaaaaaccta tggctactat
     4261 gttttttctg aaatagaaaa tcaacgaaaa attttattgg aaacccatac cgaaatagaa
     4321 gttatagaca agggggccgg ttcgcgggtt tttaaaggaa acaaaaggag tatacaacga
     4381 attgcgaggt actgcctcac agagcaggag gatgcacaaa tcttattcag acttggtgct
     4441 tgggcagggt caaatcacat tcttgaactg ggaacatccc ttggtgttac aactgcgtat
     4501 ctggcaacca ctccgtgtaa tttggaactg atttcgatag atgcatgcag taacaccatt
     4561 aaagaggcaa aaaaattact ttctgaattg tcagtagaga atgttactct tttaaacagc
     4621 gatttttcat tggctatcga aaacttgatt aaggaaaacc gcaaattcga ttttatatat
     4681 tttgatggcg atcacaatgg actgcgaatg agaaattatt acgagggtgt tcgcttgaat
     4741 cttgttggta aaaggtatgt aatggttttc gatgatattt cctggtcgag agatatgaac
     4801 acattttgga aatacattgc acgtaatagt gattcctgta ttatttcgac cggaacaaca
     4861 ggttatgtaa ttcatggatt tgatctccct tgtggatatt ttatgttaaa attatctaaa
     4921 acctgattca gattcaactt aatacactat tttcgttacc gggtctcaat tcaattttat
     4981 gtcaaacaat actgttattc aacttaaaga cgcctcaaag gattactatg ttggaattaa
     5041 tgtggtcaat gccttgaagt cgatttcgct gtatattgaa aaaaatgaat ttgttgcaat
     5101 aatgggaccc tccggctccg gaaaatctac tttgatgaac attatcgggt gtcttgatac
     5161 actgaccaag gggtcatata ttctgaacgg ttccgatgtt agcgatttga cagataactc
     5221 actggccaca atacgcaata aagaaattgg ctttgttttt cagactttta atttgctccc
     5281 acgatattcg gcacttgata acgtaatgtt gccattggta tatgccggag taccttcaaa
     5341 gaaaagaaag gaaatcgccg aagaggtctt gttgaatgtt ggtttgcagg atcgaatgca
     5401 ccacaaacct aacgaattgt ctggaggtca aagacaacgg gttgcagtgg cccgtgcact
     5461 tgtaaacaaa ccttccatta ttcttgctga tgaacctacc ggcaaccttg atacaaaaac
     5521 atcggaggaa atcatggagc tgattgagca aattcacaac aagggcaata ctattgtgct
     5581 ggtaactcat gaggaggata ttgccctgca tgcaaagaga attgtcagac tacgagacgg
     5641 gcttatagaa tcggatgctc ttaataataa ctgacagtgt attaatcctg aaacccgatt
     5701 tattttttca ttatttttat tcgaatcaaa agtgcatgta ttgaaaatct tgtttattat
     5761 tgtgttggtt atctaaatcc taaaaaggaa atttgtatga aaatatacac aaaaacaggt
     5821 gatgatggca ctaccggttt aataggcgga gtcagagtaa gtaaagcaga tatcagggtg
     5881 gaagcttatg gtacggtgga tgaagcaaat tcatggcttg gattaatacg ggctgtgtgc
     5941 aattatgaag aaattaatca ggaattgatc tgtattcagc gaaatttatt tactatttgt
     6001 gctcatttgg cgtctgatta caaacgagta ccccagtctg caccagcaat tcctgttttg
     6061 gatccggaat tttcgaaatt tctcgaaaaa tcaatcgata aatacaacga atcaattcct
     6121 gcgcttgaaa attttattat tcccggaggc tcacaacttt cggcaagctg ccaccttgcc
     6181 cgcgcagtat gtcggcgtgc agagcgtaga gtgaccgcgt tggccggtac cgccaaagtt
     6241 gatgcagaaa tccttgtatt tttaaacagg ctatccgatt atctctttgt attggcgcga
     6301 tatttcagtt ttaaacaaca tgttgatgaa attttttgga agaatgattt gtaaatgatt
     6361 ttttttgctt tatttcgcag accttttttt gaaccataat aataattaat ttattttttt
     6421 agaatgtact ggactttaga attagcatcg aaactagaag atgcgccatg gcctgcaaca
     6481 aaagatgagc taatcgacta cgccatcagg tccggagccc ctctcgaagt tattgaaaac
     6541 ctgaacgaaa ttgaagatga aggtgaaatc tacgagagca ttgaggacat ttggccggat
     6601 tacccgagta aagatgattt cttcttcaac gaagatgagt actaatccag aaaaactgct
     6661 aaagtggaag ctgcaaacat tttgcagctt tttttatttt agtgtaaaat ggctctaagt
     6721 atatgcaaaa aaaaaggcga catttcaaag taatacaaat catcgtttag ttaattttta
     6781 tgcagaattt ggcaattttg attgtacatt tgtgaataat caaaaacccg gaccatgaag
     6841 aagagccacc tgataccaat tctgttttcg tgtgcaactt atagtatttc gtttgcacaa
     6901 attaatgttg tccctgtaac cgaccctgtt caggcagctg ccagttttgt tttcgacgga
     6961 gtttcggtta cgaacgttgt atatacaggt gacgaaaatg catttggtag tttttcaaat
     7021 gccacgggta ttgtgcccgg attggaatca ggcctggttc ttagcactgg atcgattaat
     7081 ccagaaggtt ctgcgcccgt tggagccccg gtttcttcat ttgccaatac tattaacgat
     7141 gctcctggcc actatttact caattgcctg atatcgtcgg atacttacga tgcggcagtt
     7201 ttcgaattcg atctggttcc cattggcaat attcttgaat ttcagtatgt gtttgcttcc
     7261 gaagaatacc cggagtttgc aggctcttca tttaacgatg tatttggatt ttttatttgt
     7321 ggacccaatc caaatggcgg aaactacaat aatgtaaata ttgccattat tccaggcact
     7381 accagtccgg tagccattaa caatgttaac ggcaatgtta attcttcgtt ttatattgat
     7441 aacgaagcgc taaataaccc tgattttgtt ttcgatggct caaccgtttt acttaccgct
     7501 cagataaatg ttatagccgg gaatacctac caccttgtaa tggctatagc cgatgttgga
     7561 gacatgtgct atgactctgc tatttttctg aaagcgtcaa gcatgaagag ttacatattt
     7621 tcaggggaac aaaatcctgc aacaatcaaa ccgatggttt tttacgatgc agtaagtaag
     7681 accatttgca tacaatatgt tgacggaggc cccatccagt tacaattatt taatttacag
     7741 ggtgcaatgg ttttgtcggg tatgaatgaa tcactgagta ccgaaggcct gaaaaaaggt
     7801 gtttaccttg caaagattag ttttggagaa aatgtggcgg tcgaaaagat actgatagat
     7861 taataaatct atcttttaaa gcttttcccc ttctcaaatg cgcttccaat tacaacaata
     7921 gtagctccat tttcaaattt acgttccatt tgatcttggg tatttattcc accacccaca
     7981 atcaatgggc tattacaagc attgcttaca gctctgacca tttcgtcagg taccgactta
     8041 cgggcaccac tgcctgcttc cagatagatc agtttcatgc caagcatttc gcctgccaaa
     8101 gcggtagcaa cagcaatgtc ggttttagca gcgggaatgg ggtgtgtgtt gcttatatat
     8161 tcaaccgatg tatcgttgcc attcccaatc agcatatacc ctgtgctgat agcttcgatg
     8221 ccggatttct ttataaaagg tgcggctgta acatggtttc caataaggaa ctccgcattt
     8281 cgccccgata tgagcgataa aagtaatatg gcatcggcgt ttttactgat gtggcaggcg
     8341 ttcccgggaa ataatattac cggtttgtcg gtatatttct taataccttc aatacatgag
     8401 tttgtaccgg tgctcaaaaa gcttcctcct gcaaacaaca aatctacagc cgaaaagtcc
     8461 gaacttttta caaattctct aaaattctgc tcagaaaatt tatcgggatc aatcagcatg
     8521 gcaagcaaat gtttgccatg ctctttcgtc tccataattt gctgatatac cgttagctca
     8581 ctcatttgtg caaattaatt ataattttaa tattttgcaa cctaatcttg gatggctata
     8641 ttttcaggag cttgactgca ccatacaatc acaggcatat tttcaccaga taaattcaac
     8701 ctgcgaagaa taacaatatc cttgccctgg atttttcctt cgaaatttcc gtcatcggtt
     8761 tctttaactt ccaagctttg tttaaaatcg gaatatccat gaccatagag cttgtatata
     8821 cactccttgc agcaccaaat gagtgcaatt ttttcgatat cgttgccaca atattcagat
     8881 tcgtaattat ttaaaaatcg tggagctgtc cgcagtaaac gcacatctgg ttgttcagcg
     8941 tcaatggcaa cacacattgt tttacttata attactgcag caaatccgtc ggtatgggtt
     9001 atcccaatat gagcgttatt tataaatggt ttcccccatg tatctttata aataggatct
     9061 ttaaaccctg cgtgctgtaa acacaacctt gaagcagcaa actcttctct tcgcccgatg
     9121 tgggtatatt ttactgcttc tttttggatg ttttcaggaa gactgccggt gagtatgtca
     9181 gcagattcgg ttatttgcca gcatacaatc cgggtacctg aaatgtttaa atcagtaatc
     9241 acaggcatta tttagcccat tttaaagttt ccatgatgtg aacaatatcg gtgcggataa
     9301 aactgataac cgggtccagc gaatctttgt tcgggggaca atcaaaatat agagaacctc
     9361 ttaaaaaatg ccgggtgctg tcggttacaa aaaactgaac ggaacttgcg gcgttgcctt
     9421 tgatatcgta tagaattcca taaacttttt catcttcatt cacaaaaacc gactcttcga
     9481 tggcatcagc ctttacagta tgcttataaa caaactcccg cgattcttcc atatgagttt
     9541 caaaatcgtt gttcaactct ttataggtaa gataaataat ggcatgattc agagggaaaa
     9601 ccagattagc ccagttgagt tttattgtat cagcttgcat aaaactataa ctcggcatct
     9661 cgaatgagta cggaaaggtg gtatcagatg ttttatagtc tttttcaggg aaatcgattc
     9721 tgaaatatcc atatggacgt ggggcatagt gctttttaca ggaagatgca aataatagtg
     9781 caatgattgc aaccgacaat agaatgctac ttgttttcat gaattgtaac ccttattttt
     9841 tttattctcc gtttatcaac cgattcaata gtaaaagtaa accgactgta atttatattt
     9901 tcatttttat ggggaatatc gcctttcatt tcaagtatta gtccggcaag cgaatcgctc
     9961 tcacctctta ctgcgtcaaa taggttgctg tcaacatcca gtgttttgca gaaatcaata
    10021 atcgttgtcc tgccatcgaa ctcccacgtg ttttcactaa tctgggtaaa taatatgccg
    10081 tcggtatcgc tttcatcatt gatatcacca acaatttcct caagaatatc ctccatggta
    10141 acaatcccgc aggtgccacc gtactcatca accacaacgg ccatgtgcat tttttttgac
    10201 tggaaatctt tgagcaggtc attgattttt tttgtttccg gaataatcag gtgcgtcctg
    10261 attaattttt gccatttgaa cgatttttta tcctcaatat gaatatatgg gagtaagtct
    10321 ttaatgtaca gcacccctac aatgttatca gggttttctt tatagaccgg aattctggag
    10381 tagccggttt ccacaataac tgaaagtact ttggtaaatg tttcggaata ttcgaaggcc
    10441 gtaatatcca tgcggggctt cataatttct ttaacctcaa tattcgtgaa attgattatt
    10501 ccttcgagca tttccttttc ttcattcagc tcatccgaag ccagttcaat ggcatgcgag
    10561 aggtcatcga tactgatgtt ttgtttatga cggcgctgca ttcgcttgtt tactctgctc
    10621 g
//
LOCUS       DAOOQP010000102        10567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7835_length_10567_cov_7.317732, whole genome shotgun sequence.
ACCESSION   DAOOQP010000102 DAOOQP010000000
VERSION     DAOOQP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7835_length_10567_cov_7.317732"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..2441)
                     /locus_tag="PLK75_09965"
     CDS             complement(36..2441)
                     /locus_tag="PLK75_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39613.1"
                     /translation="MNRYFLLACLLSVCVWTQAQNCEIGGSVFDSITHEPLAYCNISV
                     LNSNDSVVSGCMSNEKGEFRIKYIQCNKHYKLMVSYTGYSPVLLDLPETLPAKYTTGN
                     VYISPTVMHLNEAVVEGTTRYMEQKFDRKVYTMNESKVLSAKSIFDLLRTLPGVTVDE
                     EGNVKYKGAPATIYIDDMPSQYIYPKTEMIPVAQVIKIEVIDAALQSGSGKGGIINIK
                     MKSMATDGISGMAQAKAATVNFKDLNSGSGFANLNYKAGNFLVFANGNYVANNEWGQS
                     ENTGTLQFGETTYQMNSNTSTTNKANSLWAYGGLRYSPSENTRMFFSLGLYDYIGSYP
                     TTGNSSLVSDATSLYYDKYSQSTLYDYTYLNKWLNAYFYHKFDTIGRELSVYAGIQDQ
                     KNDYMSEVYYYHEFINGIPVFHNTSNSTITGIASRGIWAGAYYNHPINNLTRWNVGYR
                     SWIPLSNVMVEDYTLDGDDYLPNSGTTRASTPSHTLYSRFGTTVKKWKFDAGASIDYS
                     RNKVDFQRYNEASQDTAFSVDKKYLHLTPSATIIYSADSLNDIKLTYSRNVEAPFYNQ
                     ISSYIDKQDPRNWSSGNPNLKPIVYHNIYLGWQLNKLTWNINTDLFYSLTTNEVSYLT
                     LPVNEVVYLTIPQNIEHKSSLGADISAWTSIKNKVDLNFSSSIFHTLIDAENLNENLK
                     KKSFGYSLKLSSDIRFAKRTSANIYINYYSREITVDGYSYGYFDSSVSLTQKFLRNSL
                     LLTVGVNSLLNDLLKHGSYTEYGGFQYYSYTYRTTYKPTFFISVKYNFRQGDRGTQNA
                     GKM"
     gene            2760..6971
                     /gene="cas9"
                     /locus_tag="PLK75_09970"
     CDS             2760..6971
                     /gene="cas9"
                     /locus_tag="PLK75_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791619.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOY39614.1"
                     /translation="MKKILGLDLGTNSIGWAVVNEAESESEKSSIIKLGVRVNPLTVD
                     EQQNFEKGKSITTNADRTLKRSMRRNLQRYKLRRENLIEILKENGFINDTTILSENGN
                     RTTFETYRLRAKAATEEITLEQFARVLLMINKKRGYKSSRKAKGTEDGNLIDGMEVAK
                     RLYNENLTPGQLCLQLIKAGKKSLPDFYRSDLQAEFDKIFDKQLMFHPDYLTNELKEE
                     LRGKKRDAIWAICAKYFKEKGVELTGIKRQGNTAEQKTENYIWRTLGLFEKLSLEQLV
                     IVLQQISIQINASSGYLGAISDRSKELFFTKQTVGQYQIAVLNENRNASLRNMVFYRQ
                     DYLDEFNTIWEKQAEFHKELTEDLKHEIRDIVIFYQRRLKSQKGLISFCEFENRKEEV
                     EFEGKKMTKTVGSRVIPRSSPLFQEFKIWQVLNNVEVFGKGKKNKKNNEVHLFNEKQD
                     PLEFGGKRELYQEEKELLATELSIKKELKKSDILKLLFNNAKELDMNFPSIQGNLTQA
                     TLFNAYRQIIELSGHEIDFEKKSASDIITSVEEVFSILKIDTKILHFNSDKEQGAQPM
                     YKLWHLLYSFEGDNSRTGNENLINKICELYGFEKEYACILANVSFQDDYASLSAKAIG
                     KILPYLKEGNKYDIACEYAGYRHSASSLTKEEIDSKYYKDRLDILPKNSLRNPVVEKI
                     LNQMIHIINSISDNYGKPDEIRIELARELKKNANERNELTKSIAETTKLHDEYKEILS
                     KSPFNLSYISRNDIVRYKLYEELKGNGYKTLYSNTYISPSSVFSGDFDIEHIIPQSRL
                     FDDSFSNKTLELRSINIEKGDKTALDFIKEKYGETENENSLDNFLVRIEDLYNADIIS
                     RAKYNKLKMQCKDIPDGFIDRDIRDTQYIAKYAKTMLGDYVKHVVSTTGSITDRLRED
                     WQLIDVMKELNWTKYQNLGLTEEFTNKEGHKIRRIKDWTKRNDHRHHAMDALTVAFTK
                     PSFIQYLNNLNARSDKSSSIYGIEQKELYRNEKSKLLFKPPFNLDDFRSEAKHHLENT
                     LISIKTKNKVTTQNINITKKAGGVNKKVQLTPRGQLHNETIYGSQKQYVVKEETVNAS
                     FDFEKIAEVSKKTYRDALLKRLQQFNNDPQKAFSGLNAPTKNPIWLDELHTKKIPAKV
                     KTVRFETLYTIRKDISADLKLDKVIDKHVKSILETRLKEFNGNAKQAFSNLTENPIWL
                     NKDKGIAIKRVTISGVSNAEPLHDKRNKEGKLIFDENGKKQPVDFVSTANNHHVAIYR
                     DADGNLQENVVSFYEAVARRNLGQPIIDKGYKSSEGWRFLFSMKQNEYFVFPNEHTGF
                     NPTEIDLLNPDNYHLISPNLFRVQKFGSLSSGFWFRHHLETNVNTIKELKEITYKVIQ
                     SANNLNDILKVRVNHIGQIVSVGEY"
     gene            7097..8230
                     /locus_tag="PLK75_09975"
     CDS             7097..8230
                     /locus_tag="PLK75_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY39615.1"
                     /translation="MKILANPFLISNYVSKEYFCNRENELQQLIRNAKNNANTTLISS
                     RRLGKTGLIFRLFELLGEENDMATVYVDIYASRNIEDFISLLADAILKKFPERTPVGK
                     QLLKILRGFRAVFTYDDMSGAPQVQFNYRTSGDKEYTLQQLLQFLDRQPLPVLVAIDE
                     FQQIAEYPEKNMEALLRTQIQQLKNVQFIFSGSKQHTLVEIFTNAKRPFYASTQFITL
                     TAIDKEKYRGFIRAHFETGKRSIDDEAIEYILNWTNTYTYYTQFVCNRVYFHKKINVE
                     IVKQECAAILREQEPVFLQYRKLLTVKQWDLLVALAKEERLYQLYSKDFLLRHKLGSP
                     SSIKRLADALIEKEMILEETTTGDTSYRVYDVFLMRWLQRTYQ"
     gene            8259..8906
                     /locus_tag="PLK75_09980"
     CDS             8259..8906
                     /locus_tag="PLK75_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39616.1"
                     /translation="MIPLEANKIYHIFNHANGFESLFISEENKRFFMERYATHIAPIA
                     KTYAYCLMNNHFHLLLKIKGEEEVVNHMTSKVSNFGSHAGEGNKIEYFVSRQFANFFS
                     SNTQAFNKMYGRMGSLFMKNFKREEITTEEYFRALVVYIHRNPVHHRFCNQYSDWKFS
                     SYNSFVSSTNDSDNIGLDTEFVFQAFGSMEAFGAVHAEKLEQFHFSTALDMSYKL"
     gene            8903..9820
                     /gene="cas1"
                     /locus_tag="PLK75_09985"
     CDS             8903..9820
                     /gene="cas1"
                     /locus_tag="PLK75_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOY39617.1"
                     /translation="MIKRTLYFGNPAYLSVKNEQLVINIPQSGNETADTSIKTIPIED
                     IGVVVLDNPQITLTHYAIEKLLANNCALITCNSQHLPAGLLLPLEGNSVQSERFSDQI
                     HASIPLKKQLWQQTVIAKIENQQRNLKQLHGNQAKELLFWAEKVNSGDTQNIEARAAA
                     YYWKNIFPQLNGFTRDREGIFPNNLLNYGYAILRAIIARSLVASGLLPTLGIHHHNRY
                     NAYCLADDIMEPYRPVVDRLVVETMSEIENCAFLTKEVKAKLLTIPVLDVVINGKRSP
                     LMIAAGITAASLHRCYCGESRKISYPEPV"
     gene            9828..10160
                     /gene="cas2"
                     /locus_tag="PLK75_09990"
     CDS             9828..10160
                     /gene="cas2"
                     /locus_tag="PLK75_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOY39618.1"
                     /translation="MQRFSEYRIMWVIVFFDLPTETKKERKIYADFRKRLLKGGFSMF
                     QFSIYVRHCASYENAIVHATRVKTHLPEKGHVVIMKITDKQFGEMEIYYGRKTMPPAD
                     TGTQLELF"
BASE COUNT         3551 a         1988 c         2087 g         2941 t
ORIGIN      
        1 tgataatctt attattaaaa gaacggcaat ctattctaca ttttcccggc attttgtgtg
       61 ccgcggtctc cctgcctgaa gttgtatttt accgaaataa aaaatgttgg tttgtaggta
      121 gtacgatagg tataggaata atattgaaac ccgccatact cggtataaga gccatgcttg
      181 agaaggtcgt tgagtaaact gttcactccc actgtaagca acaaactgtt ccgtaagaat
      241 ttttgggtta ggcttaccga tgagtcgaaa tacccgtaag aataaccatc aaccgtaatt
      301 tcgcggctgt agtaattgat ataaatattt gccgaagtac gttttgcaaa ccgtatatcg
      361 gagctaagct tcaacgaata gccaaagctt tttttcttca ggttctcgtt caggttttcg
      421 gcatcgatga gggtgtgaaa aatgctggat gaaaaattca gatctacttt attctttatt
      481 gatgtccatg cagatatatc cgcgcctaag ctgcttttat gctcaatatt ctgtggaatg
      541 gtaaggtaaa caacttcgtt aacaggcaat gtaagatatg aaacctcgtt cgtggtaagg
      601 ctgtaaaaca aatcggtgtt tatattccag gttagcttat ttaattgcca accaagataa
      661 atattatggt aaacaatggg ttttaggtta ggattacccg aggaccagtt tctcgggtcc
      721 tgtttgtcga tataactgct aatctgattg tagaaaggtg cttcaacatt gcgtgaatag
      781 gtaagtttaa tatcgtttaa actatcggct gaataaataa tggttgcaga gggagtaaga
      841 tgcaagtact ttttatccac cgaaaacgca gtgtcctgcg aagcctcgtt atatcgctga
      901 aaatctactt tattcctgct gtaatcaatg gaggcaccag catcgaattt ccatttttta
      961 acggtggtac caaagcgtga atacaaggta tgcgatggag tacttgccct ggtagtacct
     1021 gaattgggaa gatagtcgtc accatcgagg gtgtaatcct cgaccataac attgcttaaa
     1081 ggtatccagc tcctgtatcc aacattccag cgtgtaaggt tgttgatggg atgattataa
     1141 tatgcccctg cccatatccc cctcgaggca ataccggtta tagtgctgtt gcttgtatta
     1201 tgaaaaaccg gaattccatt aataaattca tggtaataat acacttccga catataatcg
     1261 ttcttttgat cctgaatacc ggcatacacc gataactcac gcccaattgt atcgaacttg
     1321 tgatagaaat atgcattcag ccatttgttg agataagtat agtcgtacag agtagattgt
     1381 gaatatttat cgtaatacaa acttgttgca tccgatacca gcgatgaatt tccggtagtt
     1441 ggatagctgc ctatataatc gtataaccca agcgaaaaaa acattcttgt gttttcggat
     1501 ggagaatatc gcagcccccc atatgcccac aaagaatttg ccttattcgt tgtgctggta
     1561 ttagaattca tttgatatgt ggtttcacca aattggaggg tgccggtgtt ttccgattgg
     1621 ccccactcat tattagcaac ataatttccg tttgcaaata caaggaaatt tcctgctttg
     1681 taattcaagt tggcaaagcc ggagccactg ttcagatctt taaaattaac agttgcggcc
     1741 tttgcctgtg ccatgccgct aattccatca gtggccatac ttttcatttt aatattgata
     1801 atgccaccct tacccgaacc gctttgcaat gctgcgtcta tcacctcaat tttaattacc
     1861 tgtgcaacag gaatcatttc agttttggga tatatgtatt gcgaaggcat atcgtcgatg
     1921 taaattgttg cgggagcacc tttgtatttg acatttcctt cctcatcaac cgtaacaccg
     1981 ggaagggtac ggagcaggtc gaaaatgctt ttggccgaca acactttgct ttcgttcatg
     2041 gtatatacct tgcggtcgaa tttttgttcc atataacggg tcgtgccttc caccactgct
     2101 tcgttcaggt gcataactgt aggactgatg taaacatttc cggtggtgta ttttgcgggc
     2161 aatgtttcgg gtaaatcaag caatactggt gagtaaccgg tgtagctcac catgagcttg
     2221 tagtgtttat tgcattgaat atactttatt cgaaactctc ccttttcgtt gctcatacac
     2281 cccgacacta ccgaatcgtt gctgttcagc accgaaatat tacagtaagc aagcggttcg
     2341 tgtgtgattg agtcgaaaac actgccgccg atttcgcaat tttgtgcctg tgtccatacg
     2401 caaacactta ataaacaagc caataaaaaa taacggttca ttcgcaaaat tttcatctaa
     2461 ataaatcaat tatgcccaaa ttattcgggt tgctatacta attatgaaaa ttttaacaaa
     2521 agcataagcg agcatttatt cttgcgctta ataacattgc tttctcggat gggaaaacag
     2581 atgatgacaa aaaaagcctc ccacttaggc ggagaggctt accaaatgtt ttcacaacgg
     2641 aataatcctt attgtgattt gcttcaaatt cgtacaacaa aaatacaaaa aaaaatataa
     2701 acaacaattt taatgtaaaa caatttcata tttttatacc aacaaaatca aatgttatca
     2761 tgaaaaaaat acttggactg gatctgggca caaacagtat tggatgggca gttgtaaatg
     2821 aagctgaaag tgaatcggaa aaatcttcta ttatcaagct gggagttcgt gtaaatcctt
     2881 tgactgttga tgaacagcaa aattttgaaa aaggcaaaag cattaccaca aatgctgata
     2941 gaacactaaa acgcagtatg cgccggaact tacaacgcta taaacttcgc cgtgaaaatt
     3001 taatcgaaat ccttaaagaa aacggattta tcaacgatac caccattcta tctgaaaacg
     3061 gcaaccgaac tacatttgaa acttacagac tacgtgcaaa ggcagcaacc gaagaaatca
     3121 ctttggaaca atttgcacgc gtgttgctga tgataaacaa aaagagggga tataaaagca
     3181 gccgaaaagc gaaaggaact gaagacggca acttgattga cggtatggaa gttgcaaaac
     3241 gcctttataa cgaaaatctt acgcctggac agctatgctt acaacttata aaggcaggca
     3301 aaaaatcgct ccctgatttc tatcgttctg atttacaagc cgaattcgat aaaatttttg
     3361 acaaacagtt gatgtttcac cccgattatc ttaccaatga attaaaagaa gaactcagag
     3421 gtaaaaagcg agatgcaatt tgggctattt gcgcaaaata ttttaaagaa aaaggggttg
     3481 aattgacagg cataaagcga cagggtaata ctgctgaaca aaaaacagaa aactatattt
     3541 ggcgcacact aggattgttt gaaaagttga gtttggaaca attagtaatt gtattgcagc
     3601 aaattagcat tcaaattaat gcatcaagcg gttatctggg tgcgattagc gaccgaagca
     3661 aagaattgtt tttcacaaaa caaacggttg gacaatacca aatagctgtt ttaaacgaaa
     3721 atcgtaatgc aagtttgcga aatatggtat tttaccgaca ggactattta gatgagttca
     3781 acaccatttg ggaaaagcaa gctgaatttc ataaagaact tacagaagat ttgaaacacg
     3841 aaattcgaga tattgttatt ttctatcagc gccgattaaa aagccaaaag gggttaatta
     3901 gtttctgcga attcgaaaac cggaaagaag aagttgaatt tgagggtaaa aaaatgacaa
     3961 aaactgtcgg aagtcgtgtt attccacgtt catcgccact gtttcaggag tttaaaattt
     4021 ggcaggtttt gaataatgta gaagtttttg gcaaggggaa aaagaataaa aagaataacg
     4081 aagtccattt gttcaacgaa aaacaagacc ctttagagtt tgggggaaaa cgagaactgt
     4141 atcaagaaga aaaagaatta ttggcgacag aactttcgat taaaaaagag ctcaaaaaaa
     4201 gtgatattct aaaattgctg tttaacaacg caaaagaatt ggacatgaac ttcccttcaa
     4261 tccaaggcaa cttgacacaa gcaacattat ttaatgctta caggcagata atagagctta
     4321 gtgggcacga gattgatttt gaaaaaaaat ctgcatcaga cataataaca tcagttgagg
     4381 aagtttttag catactgaaa atcgatacta aaatcttgca ttttaattcc gataaagaac
     4441 agggggcaca accgatgtac aaactttggc atttattata ttctttcgaa ggtgataatt
     4501 ctagaacagg caatgaaaat ttaatcaaca agatatgcga actttatgga tttgagaaag
     4561 aatatgcgtg catattagca aatgtatcat ttcaggatga ttatgcaagc ctatcagcaa
     4621 aggcaatagg taaaatatta ccatatctga aagagggtaa caaatatgac attgcttgcg
     4681 aatatgcagg gtatcgtcac tctgcaagtt cactgacaaa ggaagaaata gactcaaaat
     4741 attataagga tcggcttgat attttaccta aaaattcgct acgtaatcct gtagttgaaa
     4801 aaattcttaa tcaaatgata catataatta attcaataag cgataattat ggaaaacctg
     4861 atgaaatccg catcgaattg gctcgcgaat taaagaaaaa tgctaatgaa cgcaacgaac
     4921 tcacaaaatc aattgctgag acaactaagt tgcacgatga gtataaagaa atattatcaa
     4981 aatcaccttt taatctatca tatattagtc gcaatgatat tgttcgttat aaactatatg
     5041 aagaattgaa agggaacgga tataaaaccc tatattctaa tacatatatt tcaccatcat
     5101 cggtttttag cggagacttt gatatagagc atattatacc tcaatcacga ttgttcgacg
     5161 actcattttc aaataagaca cttgaattaa gaagtataaa tattgaaaaa ggggacaaaa
     5221 ctgctcttga ttttattaaa gaaaaatatg gtgaaaccga gaatgagaat agtttagata
     5281 atttcttggt tagaattgaa gatttgtaca atgcagatat tatttcccgt gcgaaataca
     5341 ataaactaaa aatgcaatgc aaagatatac ctgacgggtt tatagaccgt gatatccgtg
     5401 acacccaata tattgccaaa tatgcaaaaa ccatgttagg cgattatgtc aaacatgttg
     5461 tttctacaac gggttcaatt acagaccgct tgcgtgaaga ttggcaactt atagatgtta
     5521 tgaaagaatt aaactggacg aaatatcaaa accttggatt aaccgaagaa tttaccaaca
     5581 aagaaggaca caagataaga agaattaaag actggacaaa acgcaatgac caccgacatc
     5641 atgcgatgga tgcactcaca gttgcattta ccaaaccaag ttttatccaa tacctcaaca
     5701 atctgaatgc acgcagcgat aaaagctcaa gcatttacgg tatagaacaa aaagagctct
     5761 atcgaaacga aaaaagcaaa ttgttgttta aaccgccttt taatttggat gattttcgtt
     5821 cagaagccaa acaccacttg gaaaacacat tgatttcgat taaaaccaaa aataaagtta
     5881 ctacacaaaa tataaatatc actaaaaagg caggcggtgt aaacaaaaag gtgcagctaa
     5941 ctccacgcgg acaactgcac aatgaaacca tttacgggag ccaaaaacaa tacgtggtaa
     6001 aagaagaaac agtaaatgcg tcatttgatt tcgaaaagat tgctgaagtt tcaaaaaaaa
     6061 cttataggga tgctttgtta aagcgtttgc agcaattcaa taacgatccc caaaaagcat
     6121 tttccggttt aaatgctccg acgaaaaatc ctatttggtt ggatgaactg catacaaaaa
     6181 aaatccccgc gaaggtgaaa accgtgagat ttgaaacatt gtataccatt cgcaaagata
     6241 tttctgctga tttgaaatta gataaagtga tagataaaca tgtgaagagc attcttgaga
     6301 cccgattaaa agagtttaac ggcaatgcaa aacaagcctt ctctaatttg actgagaatc
     6361 caatttggct caacaaggac aaaggcattg ctataaaacg ggttactatt tcgggggtaa
     6421 gtaatgctga accattgcac gacaagcgaa ataaagaagg aaaactgatt tttgatgaaa
     6481 atggaaagaa acaacctgtc gatttcgtca gtacggctaa taaccaccac gttgctattt
     6541 accgcgatgc cgatggtaat ttacaggaga atgtggtttc gttttacgaa gccgttgcac
     6601 gcagaaactt aggacaaccc attattgaca aagggtataa gtcaagcgaa ggctggagat
     6661 tcctttttag tatgaagcaa aatgaatatt tcgttttccc gaatgaacat acaggcttta
     6721 atcccaccga aattgatttg ctcaaccccg ataactatca tttgataagc cccaatttgt
     6781 ttagagtaca aaagtttggg agtttatcat caggcttttg gtttagacat catttagaaa
     6841 ccaatgtcaa tacaatcaaa gaattaaagg aaataacata taaagttatt caaagtgcaa
     6901 ataatttaaa tgatattctc aaagttcgtg ttaaccatat cgggcaaata gtttcagttg
     6961 gagagtatta attatcaaac tcaacattga ttgtcttggc aacatatccc tataaaactt
     7021 atttcgcaaa atgcgtttca cattttgcga aatataaatt tgttgtatat ttgttgagta
     7081 atttaatttt atgttcatga aaatacttgc aaatcctttt ttaataagca attatgtatc
     7141 gaaggaatat ttttgcaatc gcgaaaacga gcttcaacaa ctcatcagga atgccaaaaa
     7201 caatgcaaat actacgctga tttcatcgag gagattgggt aaaacagggc ttatttttcg
     7261 tctttttgag cttttgggcg aagaaaacga tatggctacc gtgtatgttg atatttacgc
     7321 ttcgcggaat atcgaagatt ttatcagcct gctggctgat gcgatattga aaaaattccc
     7381 cgaaagaacc ccggtcggca aacagctttt aaaaatactc aggggattcc gggctgtttt
     7441 tacttacgac gacatgagcg gcgcgcctca ggtgcagttt aattaccgga cgtccggtga
     7501 taaggaatac accctgcagc agttgttgca atttctcgac cggcagccgc taccggtgct
     7561 ggtggctatc gacgagtttc agcagattgc cgaatatccc gaaaaaaaca tggaagcatt
     7621 gctgcggacc caaatacagc aactgaaaaa tgtacagttc attttcagcg ggagcaaaca
     7681 acatacattg gtcgaaattt ttaccaatgc caaacggcct ttttatgcaa gtacacagtt
     7741 tattacgcta acagccatcg acaaggaaaa atatcgtggt tttatacgtg cccatttcga
     7801 aacaggcaaa cgcagcatcg acgacgaagc catcgaatat atcctgaact ggacaaatac
     7861 ctacacctat tatacccagt ttgtatgcaa cagggtgtat tttcataaga aaataaacgt
     7921 ggaaatcgta aaacaggagt gcgctgccat attgcgggaa caggaaccgg tgtttttgca
     7981 ataccgcaaa ctgcttaccg taaagcaatg ggacctgctg gttgccctgg ctaaggaaga
     8041 acgcttgtac caattgtatt cgaaagactt tctgttgcgg cacaaactcg gaagtccttc
     8101 gtcgataaaa cgattggcag atgctttgat agaaaaagaa atgatactgg aagaaactac
     8161 gaccggcgac accagctatc gtgtatatga tgtattttta atgcgctggt tgcaacgaac
     8221 atatcagtga aaatgaaaac tgattacacc gaagccctat gatccctttg gaggccaata
     8281 aaatatacca tatcttcaac catgccaacg ggttcgaaag tttgttcatt agcgaggaga
     8341 acaaacgctt ctttatggag cgatatgcca cacacattgc gcccattgcc aagacatacg
     8401 catattgcct gatgaataat cattttcatt tgctgctgaa aataaagggg gaggaggagg
     8461 ttgtaaacca catgacttcc aaagtttcga actttggaag tcatgccgga gaaggaaata
     8521 aaatcgaata ttttgtttcg aggcaatttg ccaatttttt cagcagcaat acgcaggctt
     8581 tcaataaaat gtatggacgc atgggttcgt tgtttatgaa aaactttaaa cgcgaagaaa
     8641 ttactacaga agagtatttt agggcattgg tggtgtacat acaccgaaat ccggtacatc
     8701 accggttttg caatcagtat tccgattgga agttcagctc atacaacagc tttgtttcca
     8761 gcactaacga tagcgacaat attggtttag acactgaatt tgtttttcag gcatttggga
     8821 gcatggaggc attcggggca gtacacgccg aaaaacttga acagtttcat ttttcgactg
     8881 cactcgatat gtcttataag ctatgataaa aagaactctg tatttcggga atccggcgta
     8941 cttgtcggta aaaaacgaac aattggtaat aaacattccc cagtcgggca acgaaaccgc
     9001 cgatacatca ataaaaacga ttcctatcga agacattggg gtagtggtgc tcgacaatcc
     9061 gcaaattacc cttacccact atgccatcga aaaacttttg gccaacaatt gcgccctgat
     9121 tacctgcaac tcgcaacatc tgcctgccgg actgttgcta ccgctcgaag gaaattcggt
     9181 gcaatcggaa cggttcagcg atcaaataca tgccagtatc ccccttaaaa agcaactttg
     9241 gcaacaaacc gtaattgcaa aaatcgaaaa tcagcagcga aacctgaagc aattgcatgg
     9301 aaatcaagcc aaagaacttt tgttctgggc cgaaaaggta aacagtggcg acacccaaaa
     9361 catcgaagcc cgtgcagccg catactattg gaaaaatatt ttccctcaat tgaatggatt
     9421 cacccgcgat cgcgaaggta tttttccgaa taacctgctc aactatggtt atgctatttt
     9481 aagagccatc atagcccggt cgttggttgc cagtggactg ttgccaactc tgggaattca
     9541 ccatcacaat aggtacaatg cctattgtct tgctgacgat ataatggagc cataccgccc
     9601 tgttgttgat aggctggtgg tagaaaccat gagcgaaatc gaaaattgtg cttttcttac
     9661 caaagaagta aaagcaaaac tactcaccat accggttctt gatgtggtaa tcaatggaaa
     9721 acggagtccg cttatgattg cagcaggcat tactgcagca tcgctgcatc gctgctattg
     9781 tggagagagc cgcaagattt cgtatcctga accggtttaa ttgaaccatg caacgcttta
     9841 gtgaatatcg cattatgtgg gtaatagttt ttttcgacct tccaaccgaa accaagaaag
     9901 aacggaaaat atatgccgac tttcgaaaac gcttactcaa aggcggattt tcaatgtttc
     9961 agttctcgat ttacgtgagg cactgcgcca gttacgaaaa tgctatagtt catgccacgc
    10021 gtgttaaaac ccatcttccc gaaaaagggc atgtggtaat aatgaaaatt accgacaagc
    10081 aatttggcga aatggaaata tattacggca gaaaaaccat gccgcctgcc gatacaggaa
    10141 cgcaacttga gctgttctaa aaacagaaat ccgcccctat agagcggatt actgcagtac
    10201 aaacacctga ttttttattt ctataacacc tgttaactca ttgattatct gtcattttaa
    10261 gcattaatgg tgtttatcct atttcaaaga tacgaatttg aaagcaaatc acaacagtaa
    10321 acattcgagc catttcggca gcagtggtgt ttatcctatt tcaaagatac gaatttgaaa
    10381 gcaaatcaca acagctcttt gtttggtttc atggctaaag aggtgtttat cctatttcaa
    10441 agatacgaat ttgaaagcaa atcacaacaa gcaatgtact catgagtaca gatgtttcgg
    10501 tgtttatcct atttcaaaga tacgaatttg aaagcaaatc acaacgcatt agcagaacaa
    10561 tacgaaa
//
LOCUS       DAOOQP010000103        10553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7852_length_10553_cov_4.943323, whole genome shotgun sequence.
ACCESSION   DAOOQP010000103 DAOOQP010000000
VERSION     DAOOQP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_10553_cov_4.943323"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLK75_09995"
     CDS             <1..601
                     /locus_tag="PLK75_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39619.1"
                     /translation="TYRLMVQPTNYSQIIDLGIKGNIDRKIRFNLSGNYSKIDNMHFF
                     VIDTTTTLKNKFTAVYSNVERFSGYGEIMVVPNDRLNIYLNGHYYWYSYIRSEEKPWH
                     MPNFDISLCAKYNLTKELSIGGCVYVVGTRWSKDYSLSNPVYKMKPYFDINLQAEYKF
                     ASNFSSFIYLNNIAAQKYYIWEHYRAYGFNALAGIKYSF"
     gene            725..2725
                     /gene="gyrB"
                     /locus_tag="PLK75_10000"
     CDS             725..2725
                     /gene="gyrB"
                     /locus_tag="PLK75_10000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOY39620.1"
                     /translation="MSELDNILEPTRSTAENGNASASNNYSADSIQVLEGLEAVRKRP
                     AMYIGDIGIRGLHHLVYEVVDNSIDEALAGYCNKIDVIIHENNSISVKDDGRGIPVDM
                     HEKEGRSALEVVMTVLHAGGKFNKDSYKVSGGLHGVGVSCVNALSSHMCVKVFRDGKI
                     YQQEYECGIPMYDVKVVGESNDSGTLVTFIPDSSIFTVSEYHYDIISARLRELAFLNR
                     KILLTLTDERQKDVDGNYKIEEFYSEEGLKEFVEYLDGNREKLIEKVIHISLDRNEVP
                     VEIALQYNTSFNENIHSYVNNINTIEGGTHLTGFRRGLTRTLKTYAEESGMLSKLKFE
                     INGDDFREGLTAVISVKVQEPQFEGQTKTKLGNSEVSASVDQAVSTMLKYYLEENPKD
                     ARSIVSKVILAATARHAARKARELVQRKNVLGGSGLPGKLADCTDKNAANCELYLVEG
                     DSAGGTAKQGRDRMFQAILPLRGKILNVEKAMQHKVFDSEEIKNIYTALGVTIGTDED
                     SKAANLDKLRYHKIIIMTDADVDGSHITTLIMTFFFRYMKSLIENGHLYIATPPLYLI
                     KKGKFEKYAWTENDRKDIIAEITKDGGESGIHIQRYKGLGEMNAEQLWDTTMNPQYRT
                     LRQVSIENALEADRIFSMLMGDEVPPRREFIEANATYAKIDA"
     gene            2871..4307
                     /locus_tag="PLK75_10005"
     CDS             2871..4307
                     /locus_tag="PLK75_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39621.1"
                     /translation="MLKTFILLFAFFCIASIAAGQHFKPGASDNERHPASSEFNRSLN
                     DLKGTQTIYKPATEKVYYPDPADNSIWVYSFYKTYSYNMAGKAFQIITKSAAEENQEL
                     VTSTYSPNPYGLETERTTQIWDNSNWENQSKYTWVYNSYNAVVEGRIFLWQNNAWLPS
                     GGYQSEYSYYAEKILSETTKYFDIQIMNFVYSTKVIYTYDTNDHMTKMLNQVWTGNQW
                     ENESDQTYVWNSNLITEVLFRSYSNGILVDSSRYIDVQWHELQSVNGAFEGLFQSYTR
                     QITDNGNWVNQSKYQWEYDQFDGYSETVQVYEPETNTWYYDGRYSEFYDSYHNYTGNR
                     YELWDNGWELMSELKIVYLYDGNNNLVVKIWQQWDGDDEAFTNTAKAEFSNFVPLSEV
                     YNNTTSYIKVFPIPAHDYICIETPETQLQSSELGYTLYDITGKSVIGKTILEAKENID
                     ISSLGAGMYVIRVTDCNGTIFTTKFLKQ"
     gene            4317..4898
                     /locus_tag="PLK75_10010"
     CDS             4317..4898
                     /locus_tag="PLK75_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39622.1"
                     /translation="MRKILIATSFIIMAMATFSQPANKSIYSGGMLFLQPGYIITNNP
                     QQNIRDLSMGIGGILRIYFGRYFTTGIYGGTQKAGYHSENSDQSYISLGYGGPFLGFS
                     YKSGKFRYTISAFAGMGSVKNLHIESQTGNSLSDAWLYKYSAFTVSPLLSVDYSITER
                     LNATVQTICLTAFYDKQRVMVNPVFQIGILFSR"
     gene            complement(4927..6327)
                     /locus_tag="PLK75_10015"
     CDS             complement(4927..6327)
                     /locus_tag="PLK75_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39623.1"
                     /translation="MNKILIAITALLTAAISSAQIDPLDSPYYSDGPYTSVMVEETGI
                     SPDLLIYRPDNADNGPYPTLLFQPGANSGSNTYIDKHSYDLYLEHLASYGFVVIIINN
                     TSGGSNTSLFTNAHNWIKTQVNSGTHWMSTYVDLNRFIVAGHSNGGMNATDIILDRPD
                     EVDAIVYLASYPNPGFWGIGAQDVSDYLGKVLFLNGSEDDTSVPLIGATNDISEEAYN
                     ERFTSASCKTWAYFTGVGHGGFGDYDNPDQPVGSLGREPVTASIRHILVSFLLSQFGN
                     SYTAFNNLNNPELRPSSLGEFENSCGYSGPMDYTLILNVVPEGSGTVTGAGIYTETNQ
                     VSVSASANQGFVFLHWKDQNSIIVSELPEYQFYMPSANTMLTAYFDVDSYGSVYNAEQ
                     VMVYPNPAYDEIIINHGQYRDIRITDITGKQVYFSTGERELIRVDVSEWFDGLYLITL
                     TNGETVVTRKIMVSNR"
     gene            6582..8018
                     /locus_tag="PLK75_10020"
     CDS             6582..8018
                     /locus_tag="PLK75_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39624.1"
                     /translation="MMNTFVQKLQVSAIILVFSIIWSTSVAQPYLVKTFGTPVNDYPI
                     GFIKDSDTTLLFCGWRSLVDGLLVKTNLLGDTIFTRTYPILDVVADFIKLNSEQYLII
                     DEGNNMVSISSDGTTNWQKTGVNSNYRRYIHSAAIAFDNHFVVAGEVDIFDHLIFVPD
                     YGMDSVFQRDIYIALYNFEGELIQSDTIALSDNDDFLNDIIYYNNEYHIVGYSVNEVP
                     NLYIARINSEFNLTCHLPYDYNYIFRPKSFVIDIEGNYILTGTKWLTSSHRLDMFLMK
                     FDLEGGLIWQKYFNYTTYEYGFDEGAKVIQNLDGSISALGIVNNGSNTQQLLLTKVDA
                     GGNLLVNELIDFPGHNTACSLMELDDNKIALYGCTNYNTQGNADLLYIVCDSLGNYNV
                     GISDNNELCNTILYPNPTEGAFDVSAGSGKNIRGIAIFSVTGNLISEYKNVSSNHCNL
                     SPDLKYGTYIVRIALSDENVIYKKLFIR"
     gene            8114..9049
                     /locus_tag="PLK75_10025"
     CDS             8114..9049
                     /locus_tag="PLK75_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOY39625.1"
                     /translation="MASPGIALIKNVRILLSRIVYHLTPFSYVYWFSGNLNKLKRQMS
                     LYFMIFPKRKKLFKNELSYIMEKATEKQLHSFVIPYPFVENYLSEKIQVYKDDDNGLY
                     YVIHQGKRLYYSRDYSTESAVRENYNYILIEQDADSPHSYISECFTVNQNDTVFDIGA
                     AEGNFSLDVVERVSSLYLFETNEKWVEALQATFKPWEHKVHIINKYVCNSDSNTDISL
                     NNFMGLHSVDFIKMDVEGAEVNILRDIQNLIKENRGVKFAVCTYHNHSDAKIIEHIFT
                     ECGYKHTYSSGYMLFIHSKLRPPYFRRGLIRAYPA"
     gene            9150..9818
                     /locus_tag="PLK75_10030"
     CDS             9150..9818
                     /locus_tag="PLK75_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39626.1"
                     /translation="MKKYKPILRILPVTLLCISVLVSCNPPVTFKEPQPAGVQNSPEF
                     PKHLQGTYLSVADSSTLIISNKVMRRISLIKDTVNRHDIDTGFVQQGDTLFNPYTGET
                     FRFTEKGGTLYTSRDYIDTIFQIDYDNVLRKWKGYYFINIRYDKEAWVVKKVELNKGK
                     LSISSISALTDIEKLKEITEATDDTLAPYSFAPTKKEFSEFVIEEGFGDTEIFIRISR
                     IPME"
     gene            complement(9832..10326)
                     /locus_tag="PLK75_10035"
     CDS             complement(9832..10326)
                     /locus_tag="PLK75_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOY39627.1"
                     /translation="MENTIYNFTAISLNGKAVSMSEYSGNTILIVNTASKCGFTPQFT
                     GLEQLYSKYRDKGFVILGFPCNQFANQEPGDEKSIAEGCLLNYGVSFPMFSKIDVNGK
                     SAHPLFVFLKKTLPGFWGGRIQWNFTKFLIDSNGNPVKRFSPSTTPEKIDEYLMSYFK
                     NRLT"
     gene            complement(10329..>10553)
                     /locus_tag="PLK75_10040"
     CDS             complement(10329..>10553)
                     /locus_tag="PLK75_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39628.1"
                     /translation="CLKKTFGRIFRVAHCRSQGMVLLNNTTIFLQELLEASICFVCCD
                     DSKICFWGKYFLIVNTFLSELLLVKEINIG"
BASE COUNT         3465 a         2043 c         2040 g         3005 t
ORIGIN      
        1 tacttaccgc ctgatggtgc aacctaccaa ctattcacaa atcatcgacc ttgggatcaa
       61 agggaatata gacagaaaga tccgttttaa tttaagcgga aattattcaa aaattgataa
      121 catgcatttc tttgtaattg ataccaccac cacactgaaa aataaattta cggcagtgta
      181 ttccaatgtt gaacgtttca gcggatacgg tgagattatg gtagtaccaa acgaccgcct
      241 gaatatatat ctgaatgggc actattactg gtactcctac atcagatcgg aagaaaaacc
      301 atggcatatg cccaacttcg atatcagtct ttgcgcaaaa tacaacctca ccaaagaact
      361 tagcatagga ggctgcgtgt atgttgtcgg aacacgatgg tcaaaagatt attcactgag
      421 caatccggtg tataaaatga aaccatactt cgatattaac cttcaggcag aatacaagtt
      481 cgcttctaac ttcagcagtt tcatatacct gaacaatatt gctgctcaaa agtactacat
      541 atgggaacac tacagggctt acggattcaa tgcactggcc ggtataaaat attcctttta
      601 gtttaacata attttaatca ttttgttgat aactaaggca taaacaagag gaatagccca
      661 atttcaagtc acaatttttt ctatatttgc gtaagcaaaa taataagatt taaacttatt
      721 gataatgagc gagttagaca atattttgga accaaccaga tctactgcag agaacggaaa
      781 cgccagcgca agtaataatt attcagccga tagcattcag gtactcgaag gccttgaagc
      841 tgtaaggaaa aggccggcaa tgtacattgg cgatattggc atcagggggc tgcatcacct
      901 tgtttatgaa gtagttgaca attccattga tgaagcattg gcaggatatt gcaacaaaat
      961 tgatgttatc atccacgaaa acaactccat tagtgttaaa gatgatggta ggggcatacc
     1021 ggttgacatg cacgaaaaag agggtagatc ggctcttgaa gttgtaatga ccgtactcca
     1081 tgcaggtggt aaattcaata aagattcgta taaggtttca ggaggccttc atggtgttgg
     1141 tgtatcttgt gtaaatgcgc tctcatcaca tatgtgtgta aaagtgtttc gcgacgggaa
     1201 aatatatcag caggaatatg aatgcggcat tccgatgtat gatgttaaag tagttggaga
     1261 atcgaatgat agcggaacat tggtaacttt tattcctgat agctcgattt tcacagtttc
     1321 ggaatatcat tatgatatca tttcagcacg tttgcgggaa cttgctttcc tgaacaggaa
     1381 aatccttctc acgcttaccg atgaaaggca aaaagatgtc gatggaaatt ataaaatcga
     1441 agaattctat tctgaggaag gtcttaaaga atttgttgaa tacctcgatg gtaaccgtga
     1501 aaagcttatc gaaaaagtga tacatatttc gctcgaccgc aatgaggtac ctgttgaaat
     1561 tgcacttcaa tacaacacat ccttcaacga aaacatacat agttatgtta acaacattaa
     1621 caccatagaa ggaggaaccc accttaccgg ttttcgccgc ggactgaccc gtacgctaaa
     1681 aacctatgcc gaagagtcgg gcatgctcag taagcttaaa ttcgaaatta atggagacga
     1741 cttcagggaa ggccttactg cggtgatttc tgttaaagtg caggaacctc agtttgaagg
     1801 acaaaccaaa acaaaactag ggaactccga agtgagtgcc tctgtcgatc aggcggtaag
     1861 cactatgctg aaatactatc tcgaagaaaa ccccaaagac gcacgttcca tagtttcgaa
     1921 agtaattctt gctgcaacgg cacgccatgc tgcacgcaaa gcgagggaat tagtacagcg
     1981 aaaaaatgtg cttggaggct ccggcttacc cggtaaactc gctgattgca ccgataaaaa
     2041 cgcagccaac tgtgagttgt atctggttga aggtgattct gcaggtggta ctgccaaaca
     2101 agggcgcgac agaatgttcc aggcaatatt gcccctgcgt gggaaaatcc tgaatgttga
     2161 gaaggcaatg cagcataagg tattcgatag tgaagaaatc aaaaatatat atacagctct
     2221 cggtgttacc ataggaaccg atgaagacag taaagcagcc aatctcgata aactgcgtta
     2281 tcataaaata ataattatga ccgatgctga tgtagatgga agccacatta ccacactaat
     2341 catgacgttt ttcttccggt acatgaaaag cctcattgaa aacgggcatt tatacattgc
     2401 aacaccgcca ctttacctta ttaaaaaagg taaattcgag aaatacgcct ggaccgaaaa
     2461 cgacaggaaa gatattattg ctgaaataac taaagatggt ggagaaagcg gtattcacat
     2521 tcagcgctac aaaggcttgg gtgaaatgaa cgcagaacaa ctctgggata ctacaatgaa
     2581 cccccagtac aggacactca gacaagtaag tattgaaaat gccctcgaag ccgatcgcat
     2641 attttcgatg ctcatgggcg atgaagtgcc tccaagacgt gaattcatag aagcaaatgc
     2701 aacatatgca aaaattgacg catagttagg ctttttagtt gccgctatcc gtttattgag
     2761 atttccacga acattactat aggaacagta aaactcaatt cgtatttatt taggttttta
     2821 tcaattgctt ctatcagaaa attcattatc tttggttaaa catttgagcc atgttaaaaa
     2881 ctttcattct tttattcgca tttttttgca ttgcaagcat tgccgcaggc caacacttta
     2941 agccaggcgc aagcgacaat gagagacacc ctgcatcaag cgaattcaat agatcgctta
     3001 atgacctgaa agggactcaa acaatttaca aaccggctac cgaaaaagta tattaccctg
     3061 atccagccga caatagcatt tgggtttaca gtttttataa aacatacagc tacaacatgg
     3121 caggcaaagc atttcagata ataaccaaga gtgcagccga agaaaatcag gaacttgtaa
     3181 catcgactta ctctcccaac ccatacggat tagaaaccga gagaaccaca caaatttggg
     3241 acaacagtaa ctgggaaaat cagtcgaaat atacttgggt ttacaatagc tacaacgctg
     3301 tagttgaggg cagaattttc ctttggcaga acaatgcatg gctcccaagc ggagggtatc
     3361 aatctgaata cagttactat gctgaaaaaa tactatctga aacaacaaaa tatttcgata
     3421 tacaaatcat gaactttgtt tattccacta aggtaatata tacctatgat acgaacgacc
     3481 acatgactaa aatgctgaat caggtatgga caggcaacca gtgggaaaac gaatctgatc
     3541 agacctatgt atggaacagc aatttaatca ctgaagtttt atttcgcagc tattccaacg
     3601 gaattcttgt ggattcttca agatatatcg atgtgcaatg gcatgaatta caatcggtta
     3661 acggagcatt tgaaggactt ttccaatcat atacacgtca aataaccgac aatgggaatt
     3721 gggtaaacca gagcaaatat caatgggaat acgatcagtt tgatggatac agcgaaacag
     3781 tacaggttta tgaacccgaa accaatactt ggtattacga tggtcggtat tcggaatttt
     3841 acgattctta tcataattat acaggtaata ggtacgagct atgggacaat ggttgggaac
     3901 tgatgagtga attaaaaatt gtttacttgt acgatggaaa caataatttg gttgttaaaa
     3961 tatggcagca atgggatggt gatgatgaag catttacaaa taccgccaaa gcggaattca
     4021 gcaattttgt tcccctgagt gaagtttata ataacacaac atcgtatata aaagtttttc
     4081 cgattcctgc ccatgactat atttgcatcg aaactccaga aactcaatta cagagctctg
     4141 agttaggtta tacactgtac gatattaccg gaaaatcagt cataggcaaa accattctcg
     4201 aagcaaagga gaatattgat atttcctcct tgggtgcggg catgtatgtt atcagagtca
     4261 ctgactgcaa tggaaccatt tttaccacca aattcttaaa gcaatagctg gcattgatga
     4321 gaaaaattct gattgctact tctttcatca ttatggcaat ggctacgttt tcgcagccgg
     4381 cgaacaaaag tatttacagt gggggaatgc tttttttaca acctggttat attatcacta
     4441 ataatccgca acaaaacatt agagacctca gtatgggcat cggcggaatc ttaaggatat
     4501 attttggcag atattttaca accggtattt acggtggaac tcaaaaagca ggctatcatt
     4561 ccgagaattc cgatcaatcg tatatatccc tggggtatgg gggtccgttt ttagggttca
     4621 gttataaatc ggggaaattt cgttacacca tttcggcttt tgcggggatg ggatcagtta
     4681 aaaaccttca tatcgaatcg cagaccggta attcactctc agatgcatgg ctttataaat
     4741 attcagcatt tacggtatcg cccctgctta gtgttgacta ttccattact gaaaggctga
     4801 acgcaacagt acagacaatt tgtctaaccg cattctacga taagcaacgc gtgatggtta
     4861 accctgtttt ccagataggg atattattca gccgttaaaa acatatattt tagctgagcc
     4921 accctttcac cggttcgata ccataatttt gcgcgtaaca acagtttcac cgtttgtaag
     4981 tgttatcaga tataagccat cgaaccattc cgaaacatcc acacgaatta gttccctttc
     5041 accggtagaa aaatacactt gttttccggt aatatcagtt atacgaatat cacggtattg
     5101 tccgtgatta ataataattt catcgtaagc cggattcggg taaaccatca cctgttctgc
     5161 attatatacg ctcccatacg aatcaacatc gaaatatgct gtgagcatgg tatttgccga
     5221 aggcatataa aactgatact ccggcaattc cgaaacgatg atactgttct ggtctttcca
     5281 gtgaagaaac acaaacccct gattggcaga agcagaaacc gaaacctgat tggtttcggt
     5341 atagataccg gctccagtaa ctgtacctga cccttcgggt actacattca ggataagggt
     5401 ataatccatg ggacccgaat acccgcaact gttttcgaat tcgccgagtg atgaagggcg
     5461 taactcaggg ttgttcagat tgttaaaggc tgtataacta tttccaaact gacttagcag
     5521 gaagcttact aaaatatgac gaatacttgc cgttaccggt tcccttccca gcgaacccac
     5581 aggctgatca ggattatcgt aatcaccaaa tcccccatgc ccgacgcctg taaaatatgc
     5641 ccatgttttg cacgatgcag aagtaaaccg ttcattatac gcttcttccg aaatatcatt
     5701 ggtcgcacca atcaggggaa ctgaggtatc gtcttcgcta ccgttcagga acaaaacttt
     5761 accgagataa tctgacacat cctgagcacc aatcccccaa aaaccggggt ttggatacga
     5821 tgcaaggtaa acaatagcat caacttcgtc gggacgatca agaataatat cagtggcatt
     5881 catgccacca ttggagtgcc ctgcaacaat aaaccgattc agatcaacat aggtgctcat
     5941 ccaatgggta cccgagttta cctgagtttt tatccaattg tgtgcattgg taaacaacga
     6001 cgtattcgat cctcccgaag tattgttgat gataataacg acaaatccat agcttgccaa
     6061 atgctcaagg taaaggtcgt aggaatgttt atcaatatac gtgttcgacc ctgaatttgc
     6121 accgggctgg aagagtaatg tcggataggg accattgtca gcattatcgg gtcggtaaat
     6181 aagtaaatcg ggactaatac cggtttcttc aaccatcaca gaagtataag gtccatcgga
     6241 ataatatggc gaatctaaag gatcgatctg agccgatgaa atcgccgcgg taagcagtgc
     6301 agtaatggca ataagtattt tattcataat gtattgatat ttaggtttat gcaaagaaaa
     6361 ttaaaaaata gacaaacaca atattcaata aaaatatatc ttagaaattt ggaagaattt
     6421 tcataacctt tcttttatat ttaaattatc attaccttgt gattgatttt tatagtaagt
     6481 agtattttgt gtttatttat cccaacatat ctgaaatgaa tttgtttcaa agcaaacata
     6541 gaatagaaaa acataagacc ttttccaaaa ttaatgcaac catgatgaac acttttgtgc
     6601 agaagttaca ggtatcagcc attatattag ttttctccat tatttggagt acttctgtgg
     6661 cacaaccata ccttgtaaaa actttcggca ctcctgtaaa tgattacccg atcgggttta
     6721 ttaaagacag tgatactact ttgttgtttt gcggctggag atcgttggtc gacgggttat
     6781 tagttaaaac gaatttgctg ggtgatacaa tttttaccag aacttatcct attcttgacg
     6841 ttgttgccga ttttatcaag cttaacagtg aacaatacct aataattgat gaaggaaata
     6901 atatggtgag tatttcttcc gatggtacta caaactggca aaaaacggga gtaaactcca
     6961 attaccgtcg ttacatccac agtgcagcaa tagctttcga taaccatttt gttgttgcag
     7021 gagaagtaga tatatttgat cacttgatat ttgtacctga ctacggaatg gatagcgtct
     7081 ttcagcgaga tatttacatt gctttatata atttcgaagg agaacttatt caaagtgaca
     7141 ctatcgccct tagtgataat gacgactttt taaatgatat aatttattat aacaacgaat
     7201 atcacattgt aggatactca gtcaatgaag tgcccaattt atatattgcc agaattaaca
     7261 gtgaattcaa tcttacatgc cacttgccat atgattataa ttacatattc aggcccaaat
     7321 cgtttgttat cgatatcgaa ggtaattata ttttaacagg aaccaagtgg cttaccagca
     7381 gtcacagact ggatatgttc ctgatgaaat ttgaccttga aggcggctta atatggcaga
     7441 aatactttaa ttacacgacc tatgaatatg gttttgacga aggtgctaaa gtaatacaga
     7501 accttgacgg cagcatttca gcattaggga tagtaaataa tggatcaaat acacagcaat
     7561 tattgctgac aaaagtagat gccggcggaa atcttctggt caatgagctc atcgattttc
     7621 ccggtcataa tacagcatgt tctttaatgg aattagatga taataaaatc gctttatatg
     7681 ggtgtacaaa ctacaatact caaggcaatg ccgacttatt gtacattgta tgcgatagtc
     7741 tgggaaacta caatgtcggt atcagcgata ataatgaact ttgcaacaca atcctctacc
     7801 ccaacccaac agaaggggca tttgatgtat cggctgggag cggaaaaaac ataaggggaa
     7861 ttgccatttt ctccgttacc ggcaacctaa tatcggaata caaaaatgtc agttcaaacc
     7921 attgcaatct tagccctgac ctaaaatacg gaacttacat agtacgtata gctctttcag
     7981 atgaaaatgt aatatataaa aagctattta tccgataata taaattgtaa aactctcggc
     8041 ttttaaaatt gcgaaagatc tttgcgatgg cttaccataa tatttctatt tttgttctat
     8101 tagaaataaa tacatggctt caccgggaat agcactaata aaaaatgtca gaatattact
     8161 gagtcgcatt gtatatcacc ttaccccatt tagttatgta tattggttca gtggaaacct
     8221 gaataaatta aaaagacaga tgagtcttta tttcatgatt ttcccaaaaa gaaaaaaatt
     8281 gttcaaaaat gagttgagct atattatgga gaaagctacg gagaagcagt tgcactcatt
     8341 cgttataccc tatccatttg tagaaaacta cctgtcagaa aagattcagg tatataagga
     8401 tgatgataac gggctgtact atgtgataca tcaaggtaaa agactgtatt acagccgcga
     8461 ttatagtacc gaaagtgcag ttcgcgaaaa ctacaattac attctgattg aacaggatgc
     8521 agattctcca cacagctata taagcgaatg tttcactgta aatcagaacg ataccgtttt
     8581 cgacattggt gcagctgagg gaaatttttc gcttgatgtt gtagaaaggg taagcagttt
     8641 atatttgttc gaaacaaacg aaaaatgggt tgaagccttg caggcaacat ttaaaccatg
     8701 ggaacacaaa gtgcatatca taaacaaata tgtatgcaac agcgatagta ataccgacat
     8761 ttctcttaat aatttcatgg gcttacactc ggtcgatttt atcaaaatgg atgttgaagg
     8821 ggctgaagtc aacattctac gtgatataca gaatttaatt aaggaaaatc ggggtgttaa
     8881 gtttgctgtt tgcacatacc acaaccattc cgatgcaaaa attattgaac acatttttac
     8941 ggaatgcggg tataagcata catattcatc cggctacatg ttgtttatcc acagtaaatt
     9001 acgccccccg tactttcgac gtggacttat cagggcttat ccggcataaa taattttact
     9061 ccaataaata ttgacaattc aaatattatt tgctattaaa acttcgtttt aatattttta
     9121 cgatatacca acaaatcaat cattaagcca tgaaaaagta caaaccaatt ttacggattt
     9181 tacctgtgac acttctttgc atttcggtgt tagtttcctg taatccgccc gtcactttca
     9241 aagaacctca accggccggc gtacagaatt ctcccgaatt tccgaaacac ttacaaggga
     9301 catatctgag tgttgccgac agctcaacac tcatcatctc caacaaggta atgcggcgaa
     9361 tttcgctaat aaaagatact gtcaaccgtc atgacattga cactggtttc gttcaacaag
     9421 gcgatacatt gttcaaccct tataccggag aaacattccg atttaccgaa aagggaggta
     9481 ccctctacac aagcagggat tatattgata ctatttttca gattgattat gataatgttt
     9541 tgcgtaaatg gaaggggtat tattttatta atattcgtta cgataaagaa gcctgggttg
     9601 ttaaaaaagt tgaactgaac aaggggaagc ttagcatcag tagtatttcg gcacttaccg
     9661 atatcgagaa gctaaaagaa attaccgaag caaccgacga tacccttgcc ccatacagtt
     9721 ttgctcctac taaaaaagaa tttagcgaat ttgttataga ggaaggcttc ggcgatacag
     9781 aaatttttat tcgtatcagc cgcattccaa tggagtaaat atccgcattt attaagtcag
     9841 cctgttttta aaataagaca taaggtattc gtcaatcttt tcaggtgttg tagaaggtga
     9901 aaaacgtttt acagggtttc cgttgctatc aatcagaaat ttggtgaaat tccattgaat
     9961 tcttccgccc caaaaacccg gaagcgtctt tttcaggaat acaaacaatg gatgcgctga
    10021 ttttccattt acatcgattt tcgaaaacat aggaaaactg acaccgtaat tcagcagaca
    10081 gccttcggcg attgactttt cgtcacctgg ttcctgattg gcaaattgat tgcaggggaa
    10141 tcccaggatt acaaaaccct tgtcgcggta tttactatat aactgttcaa gacctgtaaa
    10201 ctgaggtgta aaaccacatt tgctggcagt gttaacaata agtattgtat tacccgaata
    10261 ttcgctcatt gaaactgcct ttccattcag tgaaattgca gtgaaattat aaattgtatt
    10321 ttccacagtt accctatatt tatctcttta acaagtaaca attcactcaa gaaagtgttt
    10381 acaatcagaa aatattttcc ccaaaaacat atttttgaat catcacaaca aacaaaacaa
    10441 atactagctt ccaataattc ttgcaaaaat attgttgtat tgttcaacaa taccatcccc
    10501 tgacttcgac aatgcgccac cctaaaaatt ctcccaaaag ttttcttcaa aca
//
LOCUS       DAOOQP010000104        10499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_7902_length_10499_cov_5.014267, whole genome shotgun sequence.
ACCESSION   DAOOQP010000104 DAOOQP010000000
VERSION     DAOOQP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7902_length_10499_cov_5.014267"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLK75_10045"
     CDS             <1..390
                     /locus_tag="PLK75_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39629.1"
                     /translation="TLRASTDRALFAMNKTLAEKLFFVSEKYGLGSEISVSDCFPATI
                     NHQECVTIIENAVSTTGLQINRLQLPFRWSEDFGHYSALAKSAMFGLGSGIDHTPLHM
                     AGYEFPDEIIASGIEAFAAIIKQVNGL"
     gene            complement(397..1452)
                     /gene="corA"
                     /locus_tag="PLK75_10050"
     CDS             complement(397..1452)
                     /gene="corA"
                     /locus_tag="PLK75_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOY39630.1"
                     /translation="MNTTENQVNKAGLPPGSAVYVGSKPFGNTTVNHLCYNKTDIESF
                     EGFDLARFNNAGLCNHLLLISGLHNASLIEEICRHFEINQLIIEDILNTHHIPKIEDY
                     GSYAFITLKDLEMEQGHQKITVNQYSFILMPHLVICFSESSNSIFDPVLMRLHQNSSR
                     VRAKNSDYLLFEMLDVIIDRYIVLCENLNAFTNKLEEGVYRNPAKDFLRKVLYNKKNL
                     TGTYQSLAPLYEQISKLKKTENELVSKTAFKYVEDLSDHLKYSLQTIESLRELNISIR
                     DSYTNELNLKMNKVMQVLTLVATIFIPLSFFAGIYGMNFENMPELHTRYGYFVFLGVI
                     ISIGVGLVIFFKKKKWF"
     gene            1772..2800
                     /locus_tag="PLK75_10055"
     CDS             1772..2800
                     /locus_tag="PLK75_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39631.1"
                     /translation="MKKIILLIAISVFCYQLHAAKYRVNNTGGSAQFSNIFDAHNAAS
                     AGDTIYIEGSPVLYIGITITKPLVIIGPGYFLEDNPQTQANLYDARVQGITFNTGSAG
                     SIIMGLHVWNSGASASLITVNESNIVIRRNYLDMTGPSGYSPNTIRINSGKNNIIIEQ
                     NFLELKTNGGPTIKLEGNNTGIVINNNFIFQSAGTQAINVPSTSTAQISNNVIAGSMI
                     INNSNVHNNIMTGGGITQTSCIFTNNIGNSTQFSEPDNLQNIDMNLVFDATDPSRDGK
                     YRLINDVSNPALGYGMFGVDCGMFGGNSPYMLSGIPPVPAVFFFYTSPSGSEGLGLPS
                     SIKVKTNR"
     gene            2812..4524
                     /locus_tag="PLK75_10060"
     CDS             2812..4524
                     /locus_tag="PLK75_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39632.1"
                     /translation="MKNIILFLAATGMLCFEQCVIAQEASDTVVQIEYFIDSDPGYGL
                     GTPLSFAEDQMIDMDFIADISELSINTTHMLGIRAMDGEGIWGQTTIRMFTVTNEPPS
                     EPKPELAEMEYFIDTDPGFGTASSLNVINDSIAEFTHTFDIMDLSNDTVHFVGIRAMD
                     NEGKWGQTTLRMFTVTENPPFEPKPELAEMEYFIDTDPGFGAATSLNVVNDSIAEFTH
                     TFDITDLSYDTVHFVGIRAIDNEGIWGHTYFLPFTVSNVAAALPNLTYIEYFIDSDPD
                     FGNGTKVYFDPDSVWDEEIVIDLQSQSLGNHFLGIRGYEENRKFSHTFLYDFTVVPFY
                     TIVSSFSEGGVIDPAGSIMVNEGSDADFAINPETGHHIWDVEIDGLSEGVITTYNFIN
                     VTEDHSIYASFVIDTLTITASAGENGTIDPSGTIYVLHGSDASFIITPDEHYHVADVN
                     VDGFGVGAVEVYDFVNVTANHTIHAQFDFNIFIEETSENFSDIIIYPNPAHDYFYLKY
                     PERAIQAVLSLHIFDLSGRKIIDLPISSDENRVNLSDCKSGLYLIEIVADNEVVATYK
                     LIKR"
     gene            complement(4585..6009)
                     /locus_tag="PLK75_10065"
     CDS             complement(4585..6009)
                     /locus_tag="PLK75_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY39633.1"
                     /translation="MHDFSKYIELPDNQLFAIAGDTSAALGSKVFLIGGYVRDRLLLR
                     DRNQDIDIVVSGDGIAFARELAKKLGISEVAVFKNFGTAMFHFGGVQYEFVGARTESY
                     RKESRKPMVAAGSITDDQNRRDFTINAMAVSLNKSDYGLLIDPHGGLDDLEKQIIRTP
                     LDPDITFSDDPLRMLRAIRFASQLGFDIESETFEAISRNKERIRIISDERIHTELNKI
                     LMSPRPSVGFNLLNISGLLLIVFPELDALSGIDVYNNRGHKDNFYHTLQVVDNVARKS
                     DNLWLRWAALLHDIAKPVTKKFIEGQGWTFYGHNIVGEKMVAGLFNRLKLPLNEKMKY
                     VQKIVGLHMRPIALVEEEVTDSAVRRLLFEAGNDIDDLMTLCEADITSKNEEKVAQYL
                     SNFKKVRKKLREVEEKDALRNFQPPISGEEIMQTLGLAPCKTVGDIKSAIKDAILDGI
                     IPNDKEAALDFMFEIARELGITKP"
     gene            6083..6436
                     /gene="trxA"
                     /locus_tag="PLK75_10070"
                     /pseudo
     CDS             6083..6436
                     /gene="trxA"
                     /locus_tag="PLK75_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            6526..7635
                     /locus_tag="PLK75_10075"
     CDS             6526..7635
                     /locus_tag="PLK75_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39634.1"
                     /translation="MKKLFVALALILSGLAAMSQKVQLKPVFDVNEYIDMLGIIARQV
                     DTPWTDVKIPLNDKYQFDYRSGVFGFDNRWDLWISSDSVLVIDMHATTGSTESWLENF
                     YAAMAPASGSWQVSPEKLFIYKLARDERAAVHTGWLSTLAFMGDDILHKLDSCIKLGY
                     RDLIVTGHSQGGAIAYLCTSWLRHKQLDGKFPADFRIKTYCSAAPKPGNLYYAYDYER
                     ITATGWAYNVVNPYDWIPETPFTVQATTDFSPGNPFTHARKTIKKQSFPANIIMNHVY
                     QRVNKPLNRTVRRFNHFLGKKVQRISQKRISVNPPLYDGGLNYSRTGNFIVLDADSAY
                     LDRYGRGEVRTFAHHMLGPYYYLAKQLRQKGILNK"
     gene            complement(7641..8372)
                     /locus_tag="PLK75_10080"
     CDS             complement(7641..8372)
                     /locus_tag="PLK75_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOY39635.1"
                     /translation="MKYSLVISLVLILAISCTHTDADRGTALFHRNGITTTRKAISQF
                     ELNMIAAGMVDVQKLSPEIRVDLKYTTIDNYWKSDLYGNLSKAYLHVTAAEKLCKAQA
                     LLQNEKPGYRLLIYDAARPASVQQTMWETIDIHPSQKHLYLANPARGSLHNYGMAVDL
                     TICDENGNPLDMGTEFDFFGHPAYSYLESYYLQTGQLNQQQYENRLYLANLMKRAGFS
                     PSTTEWWHYNACSIYYARQYCKIVP"
     gene            8432..>10499
                     /locus_tag="PLK75_10085"
     CDS             8432..>10499
                     /locus_tag="PLK75_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOY39636.1"
                     /translation="MSRFSIIFFSFVLFSGNVLLAQQQQLSIEDAVYGIYTKLAPDRI
                     NQLQWRPGYDQYSFVKNNALHLYDITTSLETEPVNTDSLNTKIIPLRDDSLKSFPAMT
                     WIDTERFSFSTSKAMYVYNLKSNSLEKSFTFSETAENFDFSPDCRFLAYTTGTNLNIV
                     GTDHAEINVTNDRDLNIVYGATVHRNEFGIEKGTYWSPSGKYLAFYRNDESHVTSYPL
                     VDISQRIATVDSIKYPMAGMDSEEVELAIYNTQSQKTIRIETGKPVDQYLTNIAWHPD
                     EKIIYIAVLNREQNHMKLNAYNIESGKFVKTLFEEKNDNYVEPLHPIMFLPDSKTFIW
                     QSRRDGFNHLYLYDDSGKLLKQLTTGQWEVNEVYGLNSNGKLLFFKANKENPINFDIY
                     CLDMESGKIQRLSKTPGNHNAIVQFDGTHVIDVYSSTETPACYEIIDFNGQKIKTLLT
                     AENPLKNYRLGNMRIGSIKAADNKTDLYYRIILPPDFDKSKKYPCIVYVYGGPHSQLV
                     SNRWMGGAAGWDYYMAQNGYIMFTLDNRGTANRGLQFENVIHRNLGVAETEDQMLGIE
                     YLRSLKYVDMERIGVHGWSYGGFMTITMMTSHPEIFKVGVAGGPVIDWKYYEVMYGER
                     YMDTPDENSLGYQTTSLLNKAGKLQGRLLIIHGAIDNTVVWQHSLTFIQECIKNKVLV
                     DYFVYPR"
BASE COUNT         3063 a         2247 c         2194 g         2995 t
ORIGIN      
        1 actctcaggg cttccaccga cagggccctt tttgcgatga ataaaacact tgccgaaaaa
       61 ctttttttcg tttcggaaaa atacgggctt ggctccgaaa tttcggtttc cgattgtttc
      121 ccagcaacca tcaatcatca ggaatgtgtt acaattattg aaaacgctgt ttctacgaca
      181 gggcttcaga ttaatagatt acaattacca tttcgctggt ccgaagactt cgggcattac
      241 tcagcgctgg caaaatcggc aatgtttggg ctgggatccg ggatcgatca tacaccgcta
      301 catatggcag gttatgagtt tccggacgaa attattgctt caggaattga ggcttttgct
      361 gctattataa agcaggtgaa cggtttatga aagatgttaa aaccactttt tctttttgaa
      421 aaaaatcacc aatcctacac caattgaaat aattactcct aaaaacacaa aatacccgta
      481 gcgggtatgc agctctggca tattttcgaa gttcatccca taaatcccgg caaaaaacga
      541 gaggggaata aagattgtgg caaccagtgt aagcacctgc atcaccttgt tcattttcag
      601 attcagttca ttggtgtagc tatccctgat gctgatgttc aactcgcgca gtgattctat
      661 tgtctgcagc gaatatttca ggtgatccga caggtcttcc acgtatttaa aagcagtctt
      721 cgaaacgagc tcattttcgg tctttttaag tttcgatatc tgctcatata gtggtgcaag
      781 gctctgatag gtgcctgtaa ggtttttctt gttgtagagc actttacgca gaaagtcttt
      841 tgccgggttg cggtatacgc cttcttccaa cttatttgtg aatgcgttca ggttttcgca
      901 aagaactata tagcgatcga taattacatc gagcatctca aacagcaggt aatcggagtt
      961 ttttgcccta acacggctgc tattctggtg caaacgcatc agcacagggt caaaaatgct
     1021 gttgctgctt tctgaaaaac aaataaccag atggggcatg aggatgaaac tgtattggtt
     1081 tacagttatt ttctgatgac cttgctccat ttcaaggtct ttcagagtaa tgaaagcata
     1141 actgccgtaa tcttcaattt tcggaatatg atgcgtgttc agaatgtcct caataataag
     1201 ttggttgatt tcgaaatgcc tacaaatttc ttcgataagt gaagcattgt gcaatcccga
     1261 gattaatagc aaatgattgc acaacccggc attgttaaaa cgtgccaaat caaaaccctc
     1321 aaaactttct atatcggttt tgttgtaaca caaatggttt actgtggtat tgccgaaagg
     1381 cttactgcca acatatactg cgcttcccgg aggtagtccg gctttgttga cctggttctc
     1441 ggtagtattc ataggcattg ggttgatttg caaatttaca aaactttatt cgcgaaactt
     1501 cattttcaat aacaaagtgc gctattaagt tggttaagtt ctgcgataac ttattgaaat
     1561 cccactcttt taaaataaat ctctgaatta aaattcacca atctaaaggt gcctattagc
     1621 gttgaaaaaa ctgcaaggtt ttaggtaagc cgtcatcaaa ctgataattg caagctttgt
     1681 gcaatcatat caatatttat attaaatttg cttaataaca tgagtattta aataaatatg
     1741 atacaaatat tattaaccaa aatcaaccat catgaaaaaa atcattttac ttattgcaat
     1801 ttcggtgttt tgttatcagc ttcatgcagc aaaatacaga gtaaacaaca ccggagggag
     1861 cgcacagttt tctaacattt ttgatgcgca taacgctgct tcagccggag acactattta
     1921 tattgaaggt tctcctgttt tatacatagg cataacaatt acaaaacctt tggtaattat
     1981 tggtcccggg tattttcttg aagacaatcc gcaaactcag gcaaatcttt acgatgcaag
     2041 agttcaggga atcactttta ataccggttc agcaggaagt ataataatgg gcctgcatgt
     2101 atggaattcc ggtgcctcgg caagtctgat aacagtgaac gaaagcaaca tagtaatcag
     2161 aagaaattac cttgacatga ccggaccttc ggggtattcg ccgaacacaa tcagaattaa
     2221 ctcagggaag aacaatatta ttattgagca aaatttcctt gaattaaaaa cgaacggcgg
     2281 acccacaata aagcttgagg ggaacaacac cggtatcgtg atcaataaca attttatctt
     2341 tcagagtgct ggtactcagg caataaatgt tccctccacg agcacagcgc aaatatccaa
     2401 taatgtgata gcaggcagca tgattattaa caactcaaat gtacataaca acatcatgac
     2461 cggtggcggc attactcaaa cctcatgcat ttttacgaac aatatcggga attctacaca
     2521 attcagcgaa cccgacaatc tgcaaaacat tgatatgaat ctggtttttg atgctacaga
     2581 tcccagtaga gacgggaaat acaggctgat taacgatgta tcgaatccgg cgttaggcta
     2641 tggcatgttt ggtgttgatt gcggaatgtt tgggggaaac tcaccttata tgctttcagg
     2701 catacctccc gtacctgcgg ttttcttttt ctatacctct ccatcaggat ccgaaggctt
     2761 gggattgcca tcaagtatta aagttaagac caatcgctaa aaacgatgag tatgaaaaat
     2821 ataatcttgt ttttagcagc cactggaatg ctttgttttg aacagtgtgt tattgcacaa
     2881 gaagcgagtg acactgtcgt gcaaatagag tattttattg attctgaccc cggctatggg
     2941 ttggggactc ctttaagctt tgccgaagat caaatgatag atatggattt tattgctgat
     3001 atttcagagc ttagtattaa tacaacgcac atgctgggca ttcgtgcaat ggatggcgaa
     3061 ggcatttggg ggcaaaccac catcagaatg tttacagtaa caaatgaacc tccttctgag
     3121 ccgaaacctg aacttgccga aatggagtac tttattgaca cggatccggg atttggcaca
     3181 gcttcaagtt taaatgttat taacgacagt attgccgaat tcacgcatac atttgatata
     3241 atggatctca gcaacgacac ggttcatttt gtcggaatac gggccatgga taatgaaggt
     3301 aaatggggac aaaccaccct aagaatgttt acagtaacag agaaccctcc atttgagcca
     3361 aaacctgaac ttgccgaaat ggaatacttt attgacactg accctggatt tggcgcagct
     3421 acaagcttaa atgttgttaa cgacagtatc gccgaattca cgcatacatt tgatataact
     3481 gacctcagtt acgacacagt tcattttgtt ggaatacggg caattgacaa tgaaggtatt
     3541 tgggggcaca cctacttcct gccattcact gtgagtaacg tagcggctgc ccttcctaat
     3601 ttaacttaca ttgaatattt tattgactca gatcccgact tcgggaatgg tacaaaggtg
     3661 tattttgatc cagacagtgt atgggatgaa gaaatagtca ttgatttgca atcgcagtcg
     3721 ttgggcaatc attttcttgg aattcggggc tacgaagaaa atcgcaagtt cagccataca
     3781 tttctttacg atttcacagt tgtgccattc tatactattg tttccagctt cagcgagggt
     3841 ggtgttatcg accctgcagg cagcattatg gtaaacgaag gcagcgatgc agatttcgca
     3901 atcaatcccg aaaccggaca tcatatttgg gacgttgaga ttgacggact gagcgaaggt
     3961 gttattacaa cctataattt tataaatgtt acagaagacc atagcattta cgcctcattt
     4021 gtaattgaca cattaacaat aaccgcttct gccggcgaaa atggaacgat agacccctcc
     4081 ggtacgatat atgttttaca cggttctgat gccagtttca tcatcactcc cgacgagcat
     4141 tatcacgttg ctgatgtgaa tgtggatggc tttggtgtcg gagctgttga agtttacgat
     4201 tttgtgaatg taactgccaa ccacacaatt cacgcccaat ttgatttcaa tatttttatt
     4261 gaagaaactt cggaaaattt ttccgatata ataatatatc cgaaccctgc acacgactac
     4321 ttttacctga agtaccccga gagagctatc caggcggttt tgtcattaca tattttcgac
     4381 ttatcgggta gaaaaataat agacttgcca atttcttcag atgaaaacag ggtaaatctt
     4441 tcggattgca aaagtggttt atatcttatt gaaattgttg cggataatga ggttgtggcg
     4501 acttacaagc taattaaacg atgaccgaat tatgtttcat tgggttataa cacaataatt
     4561 agcaactaat ttctctgaac caccctatgg ttttgtgatt cccaattcac gggctatttc
     4621 gaacataaaa tcgagagctg cctctttatc gtttgggata atgccatcga gaatagcgtc
     4681 tttaattgcg ctcttaatgt cgccaaccgt tttacaggga gccagaccca gggtttgcat
     4741 aatctcttct ccactaatgg ggggttgaaa gttgcgcaaa gcatcttttt cttctacctc
     4801 ccgtagtttt tttcgtacct ttttaaaatt cgataaatat tgagcaactt tttcctcgtt
     4861 tttagaggtg atatcggctt cacataatgt catcagatcg tcgatgtcgt tgccggcttc
     4921 aaacaataac cgccttaccg cagagtcggt aacttcttct tcaacgaggg caatcgggcg
     4981 catgtgaagt cccacaattt tctgaacgta tttcattttt tcgtttaacg gaagtttaag
     5041 tcggttgaac aggcctgcaa ccattttttc accaactata ttatgtccgt agaacgtcca
     5101 cccttgccct tcgataaatt tctttgtaac cggtttggca atatcgtgaa gcaatgcagc
     5161 ccagcgcagc catagattat cgctcttacg ggcgacatta tcgaccactt gcagggtatg
     5221 gtaaaaatta tctttatgcc ctctgttgtt gtacacatct attcctgaaa gcgcgtccaa
     5281 ctcaggaaaa actattaaaa gtaagccact gatatttagc aggttaaacc caacagaagg
     5341 gcgtggtgac attaaaattt tattcaattc tgtatgaatc cgttcgtccg aaataatacg
     5401 aatgcgttct ttgtttcgcg aaattgcttc aaatgtttca ctctcaatat caaaccccag
     5461 ttgactggca aaccgaattg cacgcaacat gcgcaagggg tcgtcggaga aggtgatgtc
     5521 agggtcaaga ggtgtacgta ttatttgttt ttcgaggtca tcgagccctc catgcgggtc
     5581 aatcaacaaa ccataatcgc ttttgttcag gcttactgcc attgcattta tcgtaaagtc
     5641 gcgacgattc tggtcgtcgg ttatgctacc ggctgcaacc ataggtttgc gagattcttt
     5701 acggtacgat tcggtgcgtg cccctacaaa ctcatattga acacccccga aatgaaacat
     5761 ggctgtaccg aaattcttaa acacggcaac ttcactaata cctagttttt ttgcaagctc
     5821 tctcgcaaaa gcaattccat caccagaaac aacaatatca atatcctgat tcctgtcgcg
     5881 cagcaacaac ctgtcgcgca cataacctcc tataaggaac actttgctcc caagggctgc
     5941 agatgtatct cctgcaattg caaataactg attatcagga agttctatgt atttcgaaaa
     6001 atcatgcata cccggtgaac attataacag cttgcttgtt tatcatcaaa caaaaataat
     6061 aaactttaca aaaatggcag aacatttaac aaagcaaagc tttatcgaaa aagttttcga
     6121 ttatgaaaag gaaaaagagt ggaaattcaa gggtagtatc cctgctgtag ttgactttta
     6181 tgccgactgg tgcggtccat gcaaaatggt tggacctgta attgaagaat taagcaaaga
     6241 gtataaaggt aaaatcgatt tttataaagt cgacaccgaa gatcagcagg aactggcatc
     6301 ggtttttggc atcagaagca tcccttcgct acttttcata cctgttgacg gacaacccca
     6361 aatggctgcc ggagcattac caaagaactc ttttgagagc gctttcaaag atatttttgg
     6421 tgttgaaaaa gcctgatttg tgcttataaa aaagaatttg ctttagctac agcttttagg
     6481 tttcaaatct gagcttttcg gtatatttgt aaaaacctta ccggtatgaa aaagctgttt
     6541 gtggcacttg ctcttattct ttcgggcctt gctgcaatga gtcagaaggt gcaactgaag
     6601 cccgttttcg atgtgaacga atacatcgat atgctcggaa ttattgcacg gcaggtcgac
     6661 acgccctgga cagacgttaa aatacccctg aacgataaat atcaattcga ttaccgttcc
     6721 ggggtatttg gctttgataa ccgttgggat ttgtggataa gttccgattc ggtgttggtg
     6781 atcgatatgc acgccacaac cggtagcacc gagagctggc tcgaaaactt ttatgcagca
     6841 atggcacctg cttccggttc gtggcaggtt tcccccgaaa agctgtttat ttacaaactt
     6901 gcccgcgacg agcgcgctgc agtgcatacc ggttggctga gtacccttgc ctttatgggc
     6961 gatgatattc tccataaact ggattcctgc atcaagcttg ggtatcgcga tctgatagtt
     7021 accgggcata gtcagggcgg tgccattgcc tacctgtgca cctcatggtt gcgacataag
     7081 cagctcgacg gcaaatttcc tgcagatttc cgcattaaaa cctattgcag tgctgctccc
     7141 aagcccggaa acttgtatta cgcctacgat tacgaaagaa taactgcaac cggctgggct
     7201 tataatgtgg ttaatcccta cgactggatt ccagaaaccc cctttactgt tcaggcaacc
     7261 actgacttca gtccgggaaa cccatttacc catgcccgta aaacgataaa aaagcagtcg
     7321 ttccctgcca atattatcat gaatcatgtg tatcaacgcg tcaataagcc actgaacaga
     7381 acggtaaggc gctttaatca tttccttggt aaaaaagttc agcgtatttc tcaaaaacgc
     7441 atttcagtca accctccact ttacgatgga ggtttgaatt attcccggac tggaaatttc
     7501 attgttctcg atgccgattc ggcgtatctc gaccggtatg gtcgcggaga agtacgcact
     7561 tttgcccatc atatgctggg cccgtattat tatctcgcaa aacagttacg tcagaagggc
     7621 attttgaaca aataagcgca tcagggaaca atcttgcaat attgccttgc gtaatatatc
     7681 gaacaggcat tgtagtgcca ccattcggtg gtagatggag aaaaaccggc acgcttcatc
     7741 aggtttgcta gatatagcct gttttcgtat tgctgctgat tgagctgacc tgtctgcaaa
     7801 tagtagcttt ctaaatagct gtaggccgga tgaccgaaaa agtcgaactc ggttcccata
     7861 tccaatgggt ttccgttctc atcacaaatg gtaagatcga ctgccatacc ataattgtgc
     7921 agactgcccc ttgcggggtt tgccagatac agatgttttt gcgaggggtg aatgtcgatg
     7981 gtttcccaca tggtttgctg aacacttgcc ggccgcgcag catcgtaaat cagtaaccgg
     8041 taaccgggtt tttcgttttg taacagggct tgtgccttgc aaagtttttc cgcagcagta
     8101 acatgaagat atgctttgga taaattgcca tataaatctg atttccaata attatcaata
     8161 gtagtatatt tcaaatcaac acgaatttcg ggcgacaatt tctgaacgtc aaccataccg
     8221 gcagcaatca tattcaactc gaattggctt atggctttac gtgttgtagt tattccattt
     8281 cggtgaaaca aagcagtccc cctatcagca tcggtatggg tgcatgatat tgccaagata
     8341 aggacaagag atatgaccaa actatatttc aattctttca ggtttgcaat aaccgattat
     8401 atttgcataa aagttttcaa aattaatgat tatgagtcgt ttttcaatca ttttcttttc
     8461 atttgtgctg ttttcgggga atgtcctgct tgcacagcaa caacaactga gcattgaaga
     8521 tgcagtatat ggcatataca ccaaacttgc gcccgacagg atcaaccaac tgcagtggcg
     8581 gcctgggtac gaccagtaca gctttgtaaa aaacaatgcc ctccacttat acgatataac
     8641 cacatcgctt gaaactgagc ctgtgaacac cgattcgctg aataccaaaa ttattccctt
     8701 acgcgatgac agcctgaaaa gctttcctgc aatgacatgg atcgataccg aaaggttttc
     8761 gttttctact tcaaaagcga tgtatgtgta taatctgaaa tcgaattcac tcgaaaaatc
     8821 gttcacattc agtgagacag ccgaaaactt cgacttcagt ccagattgcc ggtttcttgc
     8881 ttacacaaca ggaaccaatc tgaatatagt tggaaccgat cacgcagaaa taaatgtaac
     8941 caacgaccgc gatttgaata ttgtgtatgg cgctacggtg catcgaaacg aattcggtat
     9001 tgaaaaaggt acttattgga gcccatcggg caaatacctg gctttttacc gtaacgacga
     9061 gtcgcatgta accagctatc cacttgttga catctcccag cgcatagcca ctgtcgacag
     9121 tataaaatac cctatggccg gaatggacag cgaagaggtt gagctcgcaa tttataatac
     9181 ccaatcgcag aaaaccattc gtattgaaac aggcaaacct gtcgaccaat acctgaccaa
     9241 tatcgcatgg catcccgatg aaaagatcat ctacattgca gtgctcaaca gggagcagaa
     9301 tcacatgaaa ctgaacgcct acaacataga atcagggaag tttgttaaaa cccttttcga
     9361 agaaaagaac gacaattacg ttgagccttt gcaccccata atgtttttgc ccgatagcaa
     9421 aacattcatc tggcaaagca gacgcgacgg gtttaatcat ctgtatcttt acgacgattc
     9481 cgggaaattg ctgaagcaac ttaccacagg gcaatgggag gtgaacgaag tttatggact
     9541 gaacagcaat ggcaaattat tgtttttcaa ggccaacaaa gaaaacccaa taaatttcga
     9601 tatctactgc ctcgatatgg aaagcggtaa gattcaacgc ttatcaaaaa cacctggtaa
     9661 ccacaatgcc atcgttcagt ttgatgggac ccatgtgatt gatgtgtata gcagcaccga
     9721 aacacctgcc tgttatgaaa tcatcgactt taacgggcaa aaaataaaaa ctctgctcac
     9781 tgccgaaaac cctctgaaaa actatcggct tgggaacatg cgtatcggaa gcattaaagc
     9841 cgccgataac aaaaccgatt tgtattatcg catcattctg ccgcctgatt tcgataaatc
     9901 gaaaaaatat ccctgcattg tgtatgtgta cggtggacct cattcgcaac tggtttcgaa
     9961 ccgatggatg ggaggtgctg cgggctggga ttactatatg gcacaaaacg gctacatcat
    10021 gttcacgctc gataaccgag gcaccgcaaa ccggggattg caattcgaga acgttattca
    10081 ccgcaacctt ggggtagctg aaaccgaaga tcaaatgctt ggcatcgaat acctacggtc
    10141 gctcaagtac gttgacatgg aacgtatcgg cgtgcatggc tggagttatg gtggttttat
    10201 gaccattaca atgatgacct cccacccaga gatttttaaa gtaggtgttg ccggcggacc
    10261 cgtaatcgac tggaaatact acgaggtgat gtatggagag cgctacatgg acactcccga
    10321 tgagaattcg ctgggatacc agaccaccag ccttctgaat aaagccggca aattgcaggg
    10381 cagattgctg atcattcacg gtgccatcga caatacggtg gtatggcagc acagcctgac
    10441 ctttattcag gaatgtatta aaaacaaggt gctggtcgat tattttgtgt acccacgcc
//
LOCUS       DAOOQP010000105        10373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8013_length_10373_cov_5.770983, whole genome shotgun sequence.
ACCESSION   DAOOQP010000105 DAOOQP010000000
VERSION     DAOOQP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8013_length_10373_cov_5.770983"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="PLK75_10090"
     CDS             <1..1558
                     /locus_tag="PLK75_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOY39637.1"
                     /translation="LFSENNKQRFVAVWAILHKNNEGGRYCEGFVQDVSDTLLQQAEN
                     NALIADALNRNASLLLPLIDIAKAAPVCNPDTPVTTAARLMTASDSKCIFVQSECDKT
                     IGMVTDADIRSRWIESGRNFDKTTGEIMTAPVIAFSSGQTLFDALLTMQKMNIDVLPV
                     LDDAGNIRSYVAGSEIVSRLANSGEWIIRSVNNASHASELAGMQFRLRKMVELMFNTG
                     IHARYISHVISQVSDAVIARLGDLIIREIGQPPADFAFLIFGSVGRNEQSLATDQDNA
                     LVYADADDERGKKAYAEYYNTFARKMNDYLMQCGYRSCKGGYMACNTKWCRPFGEFKN
                     YFNEWINKPVPQNLLEAEVFFDFRLGYGNQELAEAMKNYMLECMLNSEPFFYQMAANT
                     ASIKLPAQISGTIDIKKLMLPLVNIVRLYALKAQLSQSNTFQRIAELYQHNHLANDDC
                     TMLTKTYDFLLKLRFLNQLFQLSNGRVPDNLLEVSILNDTEIQQVKQAVSVIQGFYTR
                     LNYDFKHAIG"
     gene            complement(1555..2244)
                     /locus_tag="PLK75_10095"
     CDS             complement(1555..2244)
                     /locus_tag="PLK75_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY39638.1"
                     /translation="MKFSCKVNCDNCTARCDIYNSIRDSGLHFDDFNPLQAIYKKHET
                     VCKQGSDVTHAIYLVSGSAKLYIEGINHRNIIMYIMRPSSYIGLLSFFESEKYCYTVT
                     TLEESKICMIDLELVQQLYKENHAFFLKLNQAFARSTASILHKIITLNQKQARGRLAE
                     NLLYLSDLHGGSIFNMGITRRELGEMCAISEENTVRVLREFCNENIIHMEGRRLSILD
                     RKLLKKLSEIG"
     gene            2375..3145
                     /locus_tag="PLK75_10100"
     CDS             2375..3145
                     /locus_tag="PLK75_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HOY39639.1"
                     /translation="MHETNNSYSFSLFKPLNKYGRSNRNLIIILILVWFVAIFGFQVL
                     LMILQEPTPEKALLTYQAVSGKVKSGTSELVDKQQFINALIMVSGKSLIKPDDRIILH
                     KAISWAVYDIIGEDQEAKVAGYVAQLSETRTRLATADDTEYVGLQAQLTELKANINLL
                     ANEPTGVDPMNLKASILPYSLNTEKQILSDQDWKRLDQMMSFYLTHNQSVLTNASFLG
                     FPFHYFYTSEFLLFLFVGICLFYSYRITQLQKKYSVQE"
     gene            3167..4846
                     /locus_tag="PLK75_10105"
     CDS             3167..4846
                     /locus_tag="PLK75_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HOY39640.1"
                     /translation="MKKTIISLLLLTSPLLLMAGNLTQLEGSFKVGPAIILIAMLVVF
                     VVVGFMSRAKDTTDYYAAGRKISNVSSGMAIGANWMSAASVLGLSGMMYLSGYNGLAY
                     VVGWTGGYVLLLILMAGQIRKYGKFTASDFIGDRYYSQGLRATSAVFAIFISFAYCVG
                     QFGGVGLMFKWILGIDYEWAVIIGASVVLFYTMISGMIGATKNMVIQYIIIIVTFLIP
                     TFMLTYKFDYFWVIPQFGYGTVVTDIIQGIPAPNIADGAALLNAYGHNVSIVPSPEFA
                     MPWDPATGSTAFQWVAICFSLMLGTAGLPHVLQRFYITPTIRDARLSCVWGLFFICLL
                     YWSTPIYSVFGKLFSSNPEVGNLASDAIVVYTAQLGNIPPLIVGFLAAGAISAAFSTI
                     SGLLVAGSSAFSHDIYVKILKPKASPKQALRMARIATIIMGLLVGAVAMLKLGMIAQL
                     VAVAFSMAACTIFPLFLLGIWWSGSNRAGATAGISVGIAISLFALTYFIAGKAGTQLP
                     GHKFIEYWLNVWYFAWIGAPLAIITNIVVSKFTKSTPLEIRKFLVEKVHSS"
     gene            4908..5669
                     /locus_tag="PLK75_10110"
     CDS             4908..5669
                     /locus_tag="PLK75_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39641.1"
                     /translation="MIRISRHTKLILIAIAVIVGAAFVIAYVYYSAKNRAEDPRVVEV
                     KKMLARFDKLCSEKGFDYALIKLDSIETVLKNTEAYADSYELGIVYNNRGSVFLQQAL
                     YETTDSAARQSMLDAAAYWIEKSIERYTGWMKKYGAMQADSLMIITRKTFNRSDPAFA
                     GHNYERVVQKRVDDLLLAQKETPRRISVAYTNQGIIRRHQYRQDEALQCYIQAIKLWK
                     DNVTARNNFNVLMGLPPEDRSIIDRLFPPEKNKFD"
     gene            5681..6976
                     /locus_tag="PLK75_10115"
     CDS             5681..6976
                     /locus_tag="PLK75_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39642.1"
                     /translation="MKNFLIVVSFLAFSQAIFAQQEPAPKYGISFSGFVKNDFFVDTR
                     DCYTIREGHFLLYPKEVATDADNIDINDKTSFNFLSIQSRLSGKITAPDAFGAKTSGV
                     LEADFFGNENTNFTDNNGFRLRHAIVKLNWKKTELLTGQYWHPFFIPSCFSGVISFNT
                     GVPMQPFSRNPQIRLTHNLGKFGVMAAVAAQRDFTSPTGSAVLRYTAIPDISAGISYE
                     TKNEQAKTGFLIGAVAGYKMLQPVRTITVGTSTYVTNEKVQGYSATAYMKITLKPLII
                     KMQGVYGQNLFDLLMLGGFAVHEITDPLRQTVNYTTINNMAVWTEFETRGEKVQAALW
                     AGYTRNLGSNLSILNYSNTASGTESTVRGANLLSVFRVSPRFVFIRGNFNLATECELT
                     RACYASQTDGTLNRNEFGVITDIYSVHNVRVLLSVIYKF"
     gene            complement(7123..7506)
                     /locus_tag="PLK75_10120"
     CDS             complement(7123..7506)
                     /locus_tag="PLK75_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39643.1"
                     /translation="MKNHHISITVIMSCIIAGFLGCSQPHEFRKDCDLCDQVNQHEVE
                     AALIQFVYNDSNNHISKEAESRLLDKKETLHHGFKCIECGCRGNTLVYRLWCATNETY
                     ELEVQPDKNNQFTITGIQAATFEHP"
     gene            7611..7745
                     /locus_tag="PLK75_10125"
     CDS             7611..7745
                     /locus_tag="PLK75_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39644.1"
                     /translation="MKRMELQFKGGFSGKEIIGIFTTKNEAEKVAKMTLLKAGAITKF
                     "
     gene            7789..8568
                     /locus_tag="PLK75_10130"
     CDS             7789..8568
                     /locus_tag="PLK75_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOY39645.1"
                     /translation="MERLKFIFSKYFDKYNDKQPITLIKHFNKIKTIGSNSENFNLYF
                     SNSAVYSSMIEGNIIDFDSYMKYSYSGMNNTGKSFIEIEDLKKSYSFAKEHKLNLSNF
                     LKCHKILSNTIVEDNKDKGVIRDKDVYVFSNGKRKFTGASKEIVKGEMSKLFDDIEIL
                     ISRELTINQVFYYASMLHLILVQIHPFADGNGRCSRLIEKWFLSEKLGVNAWFIQSER
                     LYQKRIVSYYKNVHLGDNYTNINYDYSVPFLLMLPMALRLH"
     gene            8797..9857
                     /locus_tag="PLK75_10135"
                     /pseudo
     CDS             8797..9857
                     /locus_tag="PLK75_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611874.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10005..10295)
                     /locus_tag="PLK75_10140"
     CDS             complement(10005..10295)
                     /locus_tag="PLK75_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39646.1"
                     /translation="MKNILTLFIGLTFVACNNSTTENKSDLTTDSNKTVSVDSLYIDE
                     KTNKTEIVIKNKGDYSEKFIQGLKGLGYERFELIDSLLLINGTDTAYFPEIL"
BASE COUNT         3125 a         2026 c         2160 g         3062 t
ORIGIN      
        1 actgttttcc gaaaacaaca aacagcgatt tgtggcagta tgggcgatac ttcacaaaaa
       61 caacgagggt ggacgctatt gtgaaggttt tgttcaggat gtaagcgata ccttgctgca
      121 acaggctgaa aacaatgcgc ttattgccga tgctttgaat cgcaatgcat cactgctctt
      181 accattgatt gatattgcca aggcagcccc tgtatgcaat cccgacactc cggtaactac
      241 agcagcccga ctaatgaccg cttcagactc taaatgcatt tttgtccagt cggagtgcga
      301 taaaacaata gggatggtta ccgatgctga cattagaagt cgctggatag aatcaggaag
      361 aaatttcgat aaaactacag gcgaaattat gactgctcct gtaattgctt tcagttccgg
      421 acaaaccctt ttcgatgccc tgcttacaat gcagaaaatg aatattgatg tattgccggt
      481 acttgatgat gcgggaaata tccgcagtta tgttgcagga tcggagattg tttcacggct
      541 ggctaatagc ggtgaatgga taattcgctc ggtaaacaat gcttcccatg caagtgaact
      601 tgcaggaatg cagttccggc tgcgaaaaat ggtggagctc atgttcaata caggcattca
      661 tgcccgatat atcagccatg taatatcaca ggtttccgat gcggtaatcg cccgccttgg
      721 cgatttaatt attcgtgaaa taggtcagcc ccctgcagat tttgctttcc tgatatttgg
      781 tagcgttggg cgaaacgaac aaagccttgc aacagatcag gataatgcac tggtatatgc
      841 cgatgctgac gacgaaagag gtaaaaaagc ctacgcagag tattataata ctttcgcccg
      901 aaaaatgaat gattatctta tgcagtgcgg ataccggtcg tgtaaaggtg ggtacatggc
      961 atgcaataca aaatggtgta ggccgttcgg tgaatttaaa aactatttta acgaatggat
     1021 caacaagcct gtaccgcaga atttgctcga agcagaggtg tttttcgatt tccgccttgg
     1081 ctatggtaat caggaattgg ctgaggctat gaagaattat atgctggagt gtatgttaaa
     1141 cagcgagccg tttttttacc agatggccgc caacacagct tctattaaat tgcctgcaca
     1201 gatttccggt actattgata ttaaaaaact gatgctccca ctggtaaata ttgtacgatt
     1261 atatgcactg aaagcccagc tttctcaaag caatacattc cagcgcatcg cagaattgta
     1321 ccaacacaac catctggcaa acgacgattg tacgatgctt accaaaacct atgatttcct
     1381 gctgaaatta cgctttctaa atcagttgtt ccagttgtcg aacggaaggg tccccgataa
     1441 cctgctcgaa gtatcaattc ttaacgatac cgagatccag caggtgaaac aagcggtatc
     1501 ggtaatacag ggtttttata cacgtctcaa ctacgacttt aaacatgcaa ttggctagcc
     1561 tatctcactt aattttttta gcaatttgcg atcgagaatg cttagtctgc gaccctccat
     1621 gtgaatgata ttctcgttac aaaactccct caaaacccgt acggtgtttt cttctgatat
     1681 ggcacacatt tcgccaagtt cacggcgggt aatgcccatg ttgaaaattg aacctccatg
     1741 taaatcggat aaatataaca gattttcggc caatcgaccc cgtgcctgct tttgattaag
     1801 ggtaattatc ttgtgaagta tcgatgcggt ggaacgggca aaagcctgat tcagcttcag
     1861 aaagaatgcg tgattttctt tatatagttg ctgtaccaac tctaagtcga tcatacaaat
     1921 tttcgattct tcaagcgtgg taacagtata gcaatacttc tctgattcga aaaacgataa
     1981 caacccgatg taactgctcg gacgcataat atacataata atgttcctgt ggtttatgcc
     2041 ttcaatatac aacttagcac ttccgctcac cagatatatg gcatgcgtca catcggatcc
     2101 ctgcttgcac actgtttcgt gctttttata tattgcctga aggggattga aatcgtcgaa
     2161 atgcaatccc gaatctctta tcgaattata aatatcgcac cttgccgtac aattatcgca
     2221 atttacttta cacgaaaatt tcataatgaa ttgatatagt tgcaataata taaaaaagtt
     2281 gattagtatc aatgttccat ttcaacttat ttgaacattt tttttaaatc tatataaata
     2341 tatttgacag tattaattca taagctgata agttatgcat gaaacaaaca attcctattc
     2401 tttcagtttg tttaaacccc tgaacaaata cggaagaagc aaccggaacc tgattattat
     2461 cctcattttg gtttggttcg ttgccatttt tggatttcag gtattactga tgattcttca
     2521 agagccaact cccgagaaag cactgttaac ctatcaagcg gtttccggta aagttaagtc
     2581 aggtacatca gaacttgtag ataagcaaca gtttataaat gccttgatta tggtgtcggg
     2641 gaaaagcctt ataaaacccg atgacaggat aatcctgcac aaagcaatca gctgggcagt
     2701 atacgacatt attggcgaag atcaggaagc caaagtggcc ggatatgttg cacaactgag
     2761 cgagacccgc acccggctgg ccactgccga cgatactgaa tatgtaggtc ttcaggctca
     2821 gcttacggag cttaaggcca atatcaacct tttggcaaat gagccaaccg gagttgatcc
     2881 tatgaacctg aaagcatcta ttcttccgta cagcctgaat actgaaaagc agatattgtc
     2941 ggaccaggac tggaagcggc tcgaccagat gatgagcttt tatctgaccc acaaccagtc
     3001 ggttcttacc aatgcaagct tcttaggttt cccgtttcac tatttttata caagcgaatt
     3061 cctgttgttc ttatttgttg gcatatgcct cttctatagt taccggataa cgcaacttca
     3121 gaaaaaatat tcagttcagg aataaacaag ataaaaaaaa cacattatga aaaagactat
     3181 catatctcta ttattactta caagcccgtt gctgctgatg gcaggcaatc ttacacagct
     3241 tgaaggaagc tttaaagtgg gccctgcaat tattcttatt gccatgctgg tggtttttgt
     3301 ggttgtcggg ttcatgtcga gagcaaaaga taccaccgac tattatgctg ccggtcggaa
     3361 aatatcgaat gtgagttctg gaatggccat tggggccaac tggatgagtg cagcctctgt
     3421 gctcggtttg tcgggaatga tgtatctttc tgggtacaac ggattggcct acgtggttgg
     3481 atggacaggt ggctatgtgt tactgctgat acttatggct ggtcagataa gaaaatacgg
     3541 gaaatttacc gcgtccgact tcatcggcga cagatattat tctcagggct tacgcgcaac
     3601 cagtgctgtg tttgccattt ttatctcgtt tgcgtattgc gttggacaat ttggaggtgt
     3661 tggactcatg tttaaatgga ttctcggaat cgattacgaa tgggccgtaa tcatcggagc
     3721 atcggttgtg ctattctata caatgatttc aggtatgatt ggtgctacaa aaaatatggt
     3781 aatacaatac attatcatta ttgtgacatt tttgattcct actttcatgc tgacctataa
     3841 attcgattac ttctgggtaa tcccacagtt tggttatggc actgtagtta ccgatatcat
     3901 tcaggggata ccggcaccca atattgctga tggtgctgca ttgctcaatg catacggaca
     3961 taatgtctct atcgtgccca gcccggaatt tgctatgcca tgggatcctg ctacaggcag
     4021 tactgctttt cagtgggttg ccatctgctt ttcgctgatg ctcggaaccg ccgggcttcc
     4081 gcacgtatta caacgctttt acattacacc aacaatacgc gatgctcgac tgtcgtgtgt
     4141 ttggggcttg ttttttattt gcctcttata ctggagtacc ccaatatact ctgtttttgg
     4201 aaaattgttt tcatcgaatc cggaagttgg aaaccttgca tctgatgcca ttgtggttta
     4261 tacagctcaa ttgggtaata ttccaccctt gattgtaggt tttctggctg caggtgcaat
     4321 ttctgctgca ttctcaacta tttctggcct gcttgtagcc ggctcctccg ccttttctca
     4381 cgatatctac gtgaaaattc tcaaacccaa agccagccca aaacaagccc ttcgaatggc
     4441 tcgtattgca accatcatta tggggttgct cgtgggcgct gtggcaatgc ttaaactggg
     4501 aatgattgct cagttggttg cagtagcttt ttctatggca gcatgcacaa tcttcccact
     4561 cttcctgctt ggaatctggt ggagcggatc gaaccgcgct ggagctactg ccggtatttc
     4621 ggtaggtata gcaatttcgc tgtttgctct tacttacttt attgccggta aagcagggac
     4681 acagttgccg ggtcacaaat ttatagagta ctggctgaat gtgtggtatt ttgcctggat
     4741 aggggctcct ctggcaataa ttacaaatat tgttgtgtcg aaatttacaa aatcaacccc
     4801 gcttgaaata cgtaaattcc ttgtggaaaa ggttcatagc tcttaaatta taattatatt
     4861 acaattctag taatttcaag gtttgtaata tttaattccg aagctcaatg atccgcattt
     4921 cccgccatac aaagttaatt cttatagcaa ttgcagtgat tgtgggagca gcatttgtaa
     4981 ttgcgtatgt ttattacagt gcaaaaaatc gtgctgaaga cccccgcgtg gttgaagtta
     5041 aaaaaatgct tgccagattc gataagctat gctccgaaaa aggcttcgat tatgctctga
     5101 ttaagcttga ctctatcgaa acagtgttga aaaataccga agcctatgcc gattcgtatg
     5161 aattgggaat tgtttacaac aaccgcggca gtgttttcct gcaacaggca ctttacgaaa
     5221 caaccgacag cgcagcccgg caaagtatgt tggacgctgc cgcttattgg attgaaaagt
     5281 cgattgagag atataccggc tggatgaaaa aatacggagc gatgcaggcc gacagcctta
     5341 tgataattac caggaaaact ttcaacagaa gcgatcccgc tttcgcagga cataactatg
     5401 aaagggtggt gcagaagagg gtagacgacc tacttttggc ccagaaagag acaccccgcc
     5461 gcatctcggt tgcctatacc aatcagggta tcatacgccg gcaccagtac cgtcaggatg
     5521 aggcactgca atgctacatt caggcaatta aactttggaa agacaatgtt acggcacgaa
     5581 acaattttaa tgtgctgatg gggttgcccc ctgaggacag atcgataata gatcggttgt
     5641 ttccaccaga aaaaaataaa tttgactaaa aaataccgtt atgaaaaatt ttctgattgt
     5701 tgtaagtttt ttagcctttt cgcaggcaat atttgctcaa caagagccgg caccgaaata
     5761 tggtattagc ttttcaggtt ttgtgaaaaa cgactttttt gtcgatacgc gcgattgcta
     5821 taccatcagg gaaggacatt ttttactgta cccgaaagaa gttgcaaccg acgccgacaa
     5881 tatcgatatt aacgacaaaa catcatttaa tttcttgtcg atacaatcaa ggttgtccgg
     5941 aaaaattact gccccagatg cttttggggc caaaacttcg ggagtgcttg aggccgattt
     6001 ttttggcaac gaaaacacca attttaccga caacaatggc ttcaggctgc gacatgccat
     6061 tgtgaaactg aattggaaaa aaaccgagct tcttaccggg cagtattggc acccattttt
     6121 tatcccttct tgtttttcgg gggtaatttc gttcaacacc ggtgtaccga tgcaaccttt
     6181 ttcgcgtaac ccgcaaattc gcctgacgca taacctgggt aagtttggag ttatggctgc
     6241 cgttgctgca cagcgcgact tcacatctcc aaccggatcg gcagtattgc gttatacggc
     6301 aattcccgac atcagcgcag gaataagcta tgaaaccaaa aacgagcaag caaaaacagg
     6361 gtttttaata ggggcagtag ccggctataa aatgctgcaa ccggtgcgaa caataacagt
     6421 tggcacctca acatatgtta caaatgaaaa agttcagggg tattcggcaa ccgcatatat
     6481 gaaaattacc ctgaaaccac tcattatcaa aatgcaggga gtgtacgggc agaacctgtt
     6541 cgatttgctg atgctcggtg gctttgcagt acacgaaatt acagacccct tgcgacagac
     6601 tgtaaattat actacaataa acaacatggc tgtgtggacg gagttcgaaa cccgtggcga
     6661 gaaagtgcag gctgcattgt gggctggtta tacacgcaat ttggggtcga acctaagcat
     6721 cctgaattat tcgaatactg cttcgggaac cgaatcgact gtgcgtggtg caaacctgct
     6781 atcggtgttc agagtttcgc cacggtttgt ttttatccgg ggtaatttta acctcgccac
     6841 cgaatgcgaa ttaacccgtg catgctatgc ctcacaaacc gatggcaccc ttaaccggaa
     6901 cgaatttggt gtaattaccg atatctactc cgtacataac gtcagggttt tgctttccgt
     6961 aatctataaa ttctgaaaac aacactgaaa caaaacgcgt attgataaat agcacctttt
     7021 tctgagaatt gatgttctca tacgttttgt ttattgtatt aattttcaga gttcctataa
     7081 cttgcactca ttcgctgcgc ctgtgtacga aaccattacg cttcaagggt gctcaaatgt
     7141 tgcagcctga atacccgtta tcgtaaattg gttgtttttg tcaggttgca cttccaactc
     7201 ataggtttcg ttagttgcac accacaggcg gtacactagt gtattgcccc tgcacccgca
     7261 ctcaatacat ttaaaaccat gatgtaatgt ttctttttta tccagcagcc ggctttctgc
     7321 ctctttcgaa atatgattgt tcgaatcgtt atacacaaat tggattagcg cagcttcaac
     7381 ttcatgttgg ttcacctgat cgcataagtc acaatctttc ctgaattcat gtggttgcga
     7441 acacccaaga aaacctgcaa taatacaact catgattacc gtaatgctaa tgtgatggtt
     7501 tttcatggag ttgaaccttt attaatttat cagctcaaag ttagtaaatt taaggtagta
     7561 accaatccac caaaaattct gaattaattg atattctgtt tcatacagac atgaagagaa
     7621 tggaattaca attcaaagga ggtttttcag gtaaagaaat tattggtatt tttacaacca
     7681 aaaatgaggc tgaaaaagtt gccaaaatga ctttattaaa agctggtgca attacaaaat
     7741 tttaaatcag tttggggtgt aatgccccaa actttaaatt gaattattat ggagagatta
     7801 aaattcattt tctcaaagta ttttgataaa tacaatgaca agcaaccaat aactttgatt
     7861 aaacatttta acaaaatcaa aacaatcggc tctaattctg aaaattttaa tttatatttc
     7921 tcaaattcag cggtttattc ttcaatgatt gagggaaata ttattgattt tgatagttat
     7981 atgaaatata gttattctgg aatgaataat acgggtaaat cattcataga aattgaagat
     8041 ttgaaaaaat cgtattcatt tgcaaaagag cataaattga atttaagcaa ctttttgaaa
     8101 tgtcataaaa tattgtctaa tactatcgtt gaagacaata aagataaggg cgtaataaga
     8161 gataaagatg tttatgtttt ttctaatgga aaaagaaaat ttacaggagc aagcaaggaa
     8221 attgtaaaag gagaaatgtc aaaattattt gatgatatag aaatattgat ttcaagagaa
     8281 ctaacaatca atcaagtttt ttattatgct tcaatgttac atttgatatt agttcaaatt
     8341 catccttttg ctgacggcaa tgggcgttgc tcaagattga ttgaaaagtg gtttctatct
     8401 gaaaaattag gagtaaatgc ttggttcatt caatctgaaa ggctttatca aaaaagaatt
     8461 gtgtcatact acaaaaatgt ccatttgggc gataattata cgaacattaa ttatgactat
     8521 tcagtgcctt ttttattaat gttaccaatg gctttacggt tgcactaatt cgtttgccca
     8581 taacggattg cgtgcttgtg cttttttaat tgcacaagca cgcttgtgtg ttgttgttgt
     8641 tgtaagctcc cccaaaaata gtacgattta aaaatagaca aaagtttaaa tttttaaatt
     8701 cgcattatta tgaatgttgt tgttgttgta agctccccca aaaatagtac gatttaaaaa
     8761 tagacaaaag ttttaaattt ttaaattcgc attattatga aaagaagtaa attttcaccg
     8821 acacaggtgg ccaagattct caaggaacat gaacttggca aaagtgtaga agaattatgc
     8881 agggaatacg gctttgcccg gcagacattt tatgtatggc gtcagcgcta tggaggtatg
     8941 gatgcaaaag atttgaaaag ggtaagagag ttagaagaag aaaatgccaa gttaaagcgg
     9001 atgtatgcca atttggccat agagttagaa acagccaaat atattatcga aaaaaagtta
     9061 tagcgccttg cgataagcgc cgaataatag aagaagtagt agaagaaaaa cccataggca
     9121 taagcaaggc atgccgcaca atgggaataa gcagaagcag tttcaggtat caatcaataa
     9181 aagatgatag catgctaata tcacgtcttc atgagttgtc ggtagatcat ccaagagaag
     9241 gattttggaa gagctactat aggatacgaa actcaggtga aaaggtaaat cataaaaggg
     9301 ttcaccgggt ttacaagcaa atggggttgg ccttacggcg aaaatgcaaa aagagattac
     9361 cggcaagagt aaaggaacca ttgagccgcc caccgcattt tacacatacc tggagtatcg
     9421 attttatgag cgatgcatta attaatggca gaaaatttcg aagcttcaat gtaattgatg
     9481 actacaacag ggaaatactt tatatagaaa ccgactattc gttaaagagc agtcgagtga
     9541 tctggatact ccggcactta ataaaccgac atggcaagcc tgaaaaggtc aggatggaca
     9601 atgggcctga atttgtagca aaaatcacac agcaatggag cgaaatgaat gaaatagagt
     9661 ttaagtatat tcaacccgga aagcctactc aaaatgcata tgttgaacga tttaataaaa
     9721 cttacagaaa gcatgttctt gatgcgtatt tgtttgaaaa ccttaacgag gtaagggaga
     9781 taacagaaac ctggttgcaa gactataacc atttcaggcc tcacgattct ttaggtggat
     9841 tgccaccggt aatgtatcgg aatattaaag gaaaaaagca gcaactagaa gggctttgtt
     9901 ccgcttcggc tacgccttcg ctccacaaag cccttctaga aacagaaagt tattgaaata
     9961 tgaatcattt attctatttt tgaactgtac taaaaatggg aagcctacag tatttcagga
    10021 aaatatgcgg tgtctgtgcc gttgattaaa agcaaactat caataagttc aaacctttca
    10081 taaccaagtc ctttaagtcc ttgaataaat ttttctgagt agtcaccttt attttttatt
    10141 acaatttcag ttttattggt cttctcgtca atatataaac tgtctaccga tactgtctta
    10201 ttactgtccg ttgttaaatc gcttttgttc tctgtcgttg aattgttaca agccacaaaa
    10261 gtcagtccta taaaaagggt caatatgttt ttcattgctg tcattttaac attggctata
    10321 acggatggca ataagaaacg tagggcattt cgaagcttct acttattaag tta
//
LOCUS       DAOOQP010000106        10180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8215_length_10180_cov_4.260049, whole genome shotgun sequence.
ACCESSION   DAOOQP010000106 DAOOQP010000000
VERSION     DAOOQP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_10180_cov_4.260049"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLK75_10145"
     CDS             complement(<1..392)
                     /locus_tag="PLK75_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39647.1"
                     /translation="MKKFSLLLGFLMAVSIGFSQKKNVISCWSFMKDGFYEDAKEVID
                     KAAEDPTTKEWYKTWWYFGQTYHALGVSDKAKDKKLCNNECLDKAFEAYLKAITFNFL
                     DPEMKKLNLESEVDLIKFFGALNNSDTK"
     gene            complement(503..3118)
                     /gene="gyrA"
                     /locus_tag="PLK75_10150"
     CDS             complement(503..3118)
                     /gene="gyrA"
                     /locus_tag="PLK75_10150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOY39648.1"
                     /translation="MQVEETNSRIIPVNIETEMKSAYIDYSMSVIVSRALPDVRDGLK
                     PVQRRIMFGMSELGVFSGKAYKKSARIVGEVLGKYHPHGDSSVYLAMVRMAQNWSLRY
                     CLVDGQGNFGSVDGDSPAAMRYTEARLSKIAEEMLADIDKNTVDFMLNFDDTLEEPKV
                     LPTRLPNLLVNGASGIAVGMATNMPPHNIGNTVDAICAFIDNQEIPIDDLIGIIKAPD
                     FPTGGIIYGYEGVREAYETGRGRIVIRSKSEIEVEEGREKIVITEIPFMVNKSELIMK
                     IAELVNDKKIEGISNINDESDRSGMRIVIDIKRDSVANVVLNKLYKYTMLSSAFSVNN
                     IALVHGRPMLLNLKDLIKYFVEHRHDVIVRRTHYELDQAEKRAHILEGLLKAIDNIDE
                     VIQIIRSSATVDDARNRLMERFDFSDIQARAIVDMRLRALVALESDKIKQEYEELMAK
                     IEYYKLVLSDLSLRMKIIRDELTELKQKYSDKRKTEIVYASEELNPEDFYADEDVVIT
                     ISHLGYIKRTPVTEFRAQNRGGVGAKGSNTRDSDFIEHLYVATMHDTMLFFTQFGKCY
                     WLKVYEIPEGSRTSQGRAIQNLLNIEPNDAIKAFINVKDLKDAEYLKNRFIVMVTKKG
                     ILKKSHLEDYSRPRTNGVIALNVREDDSLLTAALIEDNTPDNPRFIIIAARNGKAIRF
                     PHNIVRPIGRTATGVKGIEIDDSDEVIGMIVVQDGQDVLVVSENGYGKKSMVEDYRIT
                     NRGGKGVKTINITEKTGKLVAVCGVYDNDHLMIINKSGITIRMAVNTLRLMSRATQGV
                     RLISLREDDAIGAVAKIVDGEAEDKTIGAAESVNPVVDVLDENNDPEAIDSEINDDND
                     FDSEAPDNNGDEDDE"
     gene            3379..5889
                     /locus_tag="PLK75_10155"
     CDS             3379..5889
                     /locus_tag="PLK75_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOY39649.1"
                     /translation="MGVRLSKEMQNVITYSREEAIRLGNEQVGPEHLLLGILRDADSL
                     GVELLNMFKIDLKTLKERIEDRIRNNDIELRDSDMDKLVYSKTAERIYRFMFIEATTM
                     QSEEVHSGHLLLSLLKIPNAVSQVFDNLGVDYTIAKMNLEQIEPIDSMSDDDDDEDAA
                     TGQDASKESASKGKGKTSDTPVLDSYGVDLTAAAEEGKLDPIVGRDVEIERIAQILSR
                     RKKNNPVLIGEPGVGKSAIVEGLAMRIVQKKVSHILFDKRVITLDMASIVAGTKYRGQ
                     FEERMKAILNELSKSNNIILFIDEIHTIVGAGGATGSLDAANMLKPSLARGELQCIGA
                     TTLDEYRQYIEKDGALERRFQKVMVEPTNEAETLTILHNIKERYEDHHNVKYTDAAIE
                     ACVKLTVRYVSDRSLPDKAIDALDEAGSRVHIKAINVPELILKLEDDLMLVKQEKLNA
                     VKNQNFEKAASFRDKERDIENKIEMAKRRWHEDLIKHRETVDEQHVAEVVAMITGVPV
                     QRIAQSEGSKLLHMDKELNGSVIGQNEAVKKVVLAIQRNRAGLKDPNKPIGTFVFLGP
                     TGVGKTQLAKVLARYLFDSEDALIRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTE
                     KVRRKPYSVVLLDEIEKAHPDVFHILLQVLDEGRLTDSLGRRIDFRNTIIIMTSNIGT
                     RQLSEYGKGIGFDTKSHKESYDIRSKSIVENALKKAFAPEFLNRIDDVIMFNSLGKSE
                     ISKIIDIELKGLFLRVKSLGYDIKISPAAKDFIAEKGFDPQFGARPLKRALQKYIEDA
                     MAEYILRSELKSGDTIMVGFNEKTGIKLRILKNDKIKG"
     gene            5957..>10180
                     /locus_tag="PLK75_10160"
     CDS             5957..>10180
                     /locus_tag="PLK75_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOY39650.1"
                     /translation="MKNKKLILWIGAAILLVVLTVVALKIFGKSGKKELSDPQKFEPY
                     ITGYTKGIISKNSDIVVQFTDKFIQEIKDKKITEDIFEFSPDIKGKTSWVDEHTVAFT
                     PAAPLKSGQEFFVTVNLKTLNKEIPKEFKEFIFKTKVKNQNFDVSISELITTDLNDFS
                     KQDVIGTIRLADVESEDKVKSLISASVGGKKQTISFEKIDDLRFSYRVTGITRTNAAQ
                     ELIISYDGKPIEVNKNGVVKYPLPALDNFSIIEVKINNFPEQSVVIQFSDPIMSEQLL
                     DGLITIGDEASGDFIIRNNIIKFIPAERIEGEYELVVSEGVKNIKGKEFEATYSKTLN
                     FESLKPQVRLVSSGVVLPTIDAGLVFPFEAVNLKAVDVRITRIFENNILQFLQVNNLD
                     GDSELERVGKVVKETTVDLSASGVTDFGEWNRFTLDLNDLIKTEPGAIYRIEIGFRKK
                     HSAYPCSDTISENPATSEVYDNDSTFWDYFENYTYDEYDYYYYDYYYDDYDKPSPCKN
                     SYYGYKNVVSRNIMASDIGLTAKLGKNNQVVAITTNLLTAKPMSGIEIKVYTFQMQVI
                     ATGKTNEDGVSTISIPAKEKPYFVVASDGKQKAYVALNYGSSLSLSEFNVSGASSGNS
                     KGFIFGERGVWRPGDSIYLSFILQDKDIPIPEGHPIVLEVTNPSGQLIYREVQNKNTW
                     GYHTFKFKTDQKSVTGNYAAKISVGSDVYYKTLPVETIKPNRLKINLDFDKSYISGDG
                     EVNATLTTQWLHGAVGKDLKAQININLDNAYTGFDKFEGYTFSDFTKRFQFTTQLLFD
                     GVTDENGKVVQPVKLDIGNQAPGVLTAYLTAKVFEKGGNFSIFEKSIVYHPYSNYIGI
                     KPDLKNSSNYVKPGSKNKMSVVIVDRNGKAITGSKELELEVFKYDYSWWYDYENTQTN
                     FASANYNNALSREKVTISNGKGSIQFEAPDVNYGSFIVIVSDKKGGHSASTTIYVSSY
                     DYYYDDGENHQNVNQIMLTANKDTFAVGEDIRINIPSVDGNALISIENGSRVLKTFWQ
                     ETHRGTTEVVFKATREMSPNIFINISLFQPHSSTGNDHPIRMYGLIPVMVEDPETHLH
                     PVITMPNELRSESTYTVKISEKDGKAMSYTIAVVDEGILNLTGFKTPDPWSHFYSREA
                     LGVNTWDMYDYVIGAFGVEIERLLSIGGDAGLKERLGDLKANRFEPMVRFIGPFHLEK
                     GKTASHTIAAPAYVGSVRTMVVARENAAYGSAEKTTPVTKPLMLLGTLPRVLGPQEEV
                     FLPISIFSMDSNIKQASVQVKTNDLVMIDGSTSQTVNFPQKGEQFIQFKLKAGNKTGV
                     AKIMVTAQSGTNIATQQIEVDIRYPITTQTDVKSGIADAGKSIEESFSATGIQGTNKL
                     CVEVYSIPPLNLEKRLNYLIQYPYGCIEQT"
BASE COUNT         3159 a         2011 c         2047 g         2963 t
ORIGIN      
        1 aatttagtgt cgctattatt aagtgcgccg aaaaatttaa ttaaatcaac ttcactttca
       61 aggttcagtt ttttcatctc cggatcaaga aagttgaaag taattgcttt cagatacgct
      121 tcaaaagctt tatcgaggca ctcattattg cacaattttt tatcctttgc cttatcgctg
      181 actcccaacg catggtaagt ctggccaaaa taccaccatg ttttatacca ctctttagta
      241 gttgggtctt cagccgcttt atcaattact tcttttgcgt cttcgtagaa gccgtctttc
      301 ataaagctcc agcaactgat tacattcttt ttctgactga aaccaatgct cacagccatc
      361 agaaaaccaa gtaacagact aaattttttc ataataacat ttaattttaa taacatgcaa
      421 aattaatact catttcaaca ttttgcaaat actttgccaa aataacattt ccaatagcgt
      481 gcataagaac atcatagtgt atctattcat catcttcatc accattgtta tctggtgctt
      541 cagaatcaaa gtcgttatca tcattgatct ccgaatctat tgcttcaggg tcgttatttt
      601 catctaaaac atcaaccact gggtttactg attctgctgc accaattgtt ttatcttcgg
      661 cttctccatc aacgatttta gcaacagcac cgattgcatc atcctccctg aggctgataa
      721 ggcgaactcc ttgtgttgct ctgctcataa gccgcaatgt gtttacagcc atacgaatgg
      781 taatgcctga tttattaata atcatcaggt ggtcgttatc gtatacaccg catacagcaa
      841 ccagtttgcc ggttttttcg gttatattga ttgtttttac ccctttcccg ccccgatttg
      901 ttatgcgata gtcttctacc atcgatttct tcccgtagcc attttccgat accacaagca
      961 catcctggcc gtcctgtacc acaatcatcc ctatcacttc atcgctgtca tcgatttcta
     1021 taccctttac cccggtagca gttctgccta taggtctcac aatattatgc gggaaacgaa
     1081 ttgccttccc attgcgggct gcaattatta tgaagcgggg gttgtcggga gtattatctt
     1141 cgataagagc agctgtaagg aggctgtcgt cttcccttac attcaaggca attacaccgt
     1201 ttgtacgtgg gcgtgaataa tcttcgagat gcgacttttt aagaatgcct tttttggtta
     1261 ccataacaat gaaccggttt ttaaggtatt ccgcatcttt aaggtcctta acattgataa
     1321 aagctttaat tgcatcattg ggttcaatgt ttagaaggtt ttgaatggcc ctgccctggg
     1381 atgtgcgact tccttcaggt atttcataca ctttcagcca gtagcatttg ccgaattgtg
     1441 taaagaaaag catagtatcg tgcatagtgg caacatacag atgttcaata aaatcagaat
     1501 cgcgggtgtt ggatcctttt gctcccaccc ctcccctgtt ttgagccctg aattctgtta
     1561 ctggggttct tttgatataa ccgagatgtg agatagtgat aacgacatct tcgtcagcat
     1621 aaaagtcctc gggattaagc tcttccgatg catacactat ttcagttttc ctcttgtcgc
     1681 tgtatttttg ctttagctct gtaagttcgt cacggattat tttcattcta agcgataaat
     1741 cggaaagtac cagcttgtaa tactcgattt tggccatcag ttcttcatat tcctgtttga
     1801 ttttatcgct ttcgagggcc accagtgctc tgaggcgcat atcaacgatt gccctggcct
     1861 gaatgtcgga aaagtcgaat ctttccatca aacgatttcg cgcatcatca acggtagccg
     1921 atgacctgat aatctgtata acttcatcta tattgtcgat agctttcagc agaccttcga
     1981 ggatatgtgc gcgtttctct gcctgatcga gttcgtagtg tgtgcggcga acaataacat
     2041 catgcctgtg ctcaacgaag tacttaatca gatctttcag gttgagcaac atgggtctgc
     2101 catgtacaag tgcaatatta tttacgctga atgccgatga cagcattgtg tatttataca
     2161 atttatttaa aaccacgttt gcgaccgaat cccttttaat gtcgattaca attcgcatcc
     2221 ctgagcggtc tgattcatcg ttaatattcg aaatgccttc aatctttttg tcgttgacca
     2281 attccgcaat cttcataatc agctccgact tattaaccat aaaaggtatt tcggtaatta
     2341 caatcttctc gcggccttct tcaacctcaa tttcgctttt tgaccggatt acaattcttc
     2401 ctctgcctgt ttcgtaggct tctctcacac cttcatagcc ataaattata cctccggttg
     2461 ggaaatcggg agcttttatt attccaatca ggtcatcaat agggatttcc tggttgtcga
     2521 taaaagcaca aattgcatca acagtatttc caatattgtg tggaggcata ttcgttgcca
     2581 tgccaacagc aataccgctg gcaccattca caagaagatt gggtaatcgg gtcggcaaaa
     2641 cctttggctc ttcgagtgtg tcatcgaaat tcaacataaa atcaaccgta ttcttatcga
     2701 tatcggcaag catttcttca gcaattttcg acaaacgggc ttcggtatac ctcatagcag
     2761 ccggactatc accgtcgaca gaaccaaagt ttccctggcc gtcaacaaga caatagcgta
     2821 acgaccaatt ctgtgccatt cttaccatag caagatacac agatgaatcg ccatgaggat
     2881 ggtatttacc cagcacctcc cccacaattc tggcagattt tttatacgcc tttcctgaaa
     2941 acacgcccag ctccgacatt ccaaacatta ttcttctctg aacaggtttt aacccatctc
     3001 ttacatccgg caaagcccta ctcacaatta ccgacatgga gtaatcgata tacgccgatt
     3061 tcatttcggt ttctatattt accggaataa tcctcgaatt tgtttcttct acctgcataa
     3121 tttactttct ttaaaaatat ctaaaccatt aataatcaga caattacatt ccttataaca
     3181 aaacatataa atttagtttt ttttacgttt ggtttttgtg taatcgcacc aacttttcaa
     3241 caatttttgt ttataaaaat atactgccat tttatgcttt taaaacaatg ttgattatta
     3301 aaacggtgtt ttattaagaa gatttttcac aattgataat aattattttt attaaatttg
     3361 ttaccaaaca aaaatattat gggagtacgc ttatcgaagg agatgcaaaa cgtaattact
     3421 tacagcagag aagaggctat acgccttggt aacgagcagg ttgggccgga gcatttatta
     3481 ttagggattc ttcgcgatgc tgattcactc ggggttgaat tgctgaatat gtttaaaatt
     3541 gatttaaaga ccctgaaaga aagaattgaa gataggatta ggaataacga cattgagttg
     3601 agagacagcg atatggataa acttgtttac agtaaaactg cagaaaggat ttaccggttt
     3661 atgtttatcg aagctaccac catgcaaagc gaagaagtac attccggaca tttattactc
     3721 agtttactta aaatacctaa tgcggtatcg caggtgttcg acaacctggg tgttgattat
     3781 acaattgcaa aaatgaatct tgaacaaatt gaacccatcg attcaatgtc ggatgatgac
     3841 gatgatgaag atgcagctac tggtcaggac gcatcaaaag aatccgcatc taaaggaaaa
     3901 ggcaaaacca gcgacacacc ggtactggat tcctacggtg ttgaccttac cgctgctgct
     3961 gaagaaggca aactcgatcc aattgttggt cgtgatgttg aaatagaacg aattgctcag
     4021 attttgagca ggagaaagaa aaacaaccct gttcttattg gggagcccgg cgttggaaaa
     4081 tctgccattg ttgaagggct tgcgatgcgc attgtacaga agaaagtttc acacattctc
     4141 ttcgacaaaa gagttattac tcttgatatg gccagtattg ttgcaggtac aaaatatcgc
     4201 gggcagtttg aggaacggat gaaagccata ttaaatgaat tatctaagtc gaacaatatc
     4261 atacttttta ttgacgaaat tcatactatt gttggtgccg gaggggccac aggctctctg
     4321 gatgcggcca atatgctgaa accgtcgctt gccaggggcg aattgcaatg cattggtgct
     4381 actacgctcg acgaatacag gcagtatatc gaaaaggacg gtgctttgga aagacgcttt
     4441 cagaaagtaa tggtagaacc tacaaatgaa gctgaaacac tgacgatcct acataatatt
     4501 aaagaacgtt atgaggatca tcataacgtg aaatacaccg atgctgcaat tgaagcatgc
     4561 gttaagctta ccgttaggta tgttagtgac cgtagtttac ccgacaaggc tattgatgcg
     4621 ctggatgaag caggtagccg tgttcatatc aaagcaatca acgtacctga attaattctc
     4681 aaacttgagg acgatctgat gcttgtgaaa caggaaaagc tgaatgctgt aaaaaatcag
     4741 aatttcgaaa aagcagcgtc gtttcgtgat aaggaaagag atattgagaa caaaattgaa
     4801 atggcaaaac gccgttggca cgaggatctt attaagcatc gtgaaactgt agatgaacag
     4861 catgttgcag aagtggttgc catgatcaca ggtgtaccag ttcaacgtat tgctcagagt
     4921 gagggctcga agttgctgca catggataaa gaactcaacg gaagcgtaat tggacagaat
     4981 gaggcagtaa aaaaagtagt actggcaatt cagcgtaatc gtgccggttt gaaagacccc
     5041 aataaaccta ttgggacttt cgtgtttttg ggtccaactg gtgtaggcaa aacacagctt
     5101 gccaaagtac ttgcccgtta tctgttcgac tcagaagacg ccctgatacg aattgacatg
     5161 agtgaatata tggagaaatt cagtgtttca cgacttgttg gagcccctcc gggatatgtg
     5221 ggttacgaag aaggcggaca gctgaccgaa aaagtccgca gaaagcccta ctccgtagtt
     5281 ttgctcgatg aaatagaaaa agcccatcct gacgtattcc atattttgct gcaggtactt
     5341 gatgaaggca gacttaccga cagtctcggt agaagaatcg acttcaggaa tacgataata
     5401 ataatgacat cgaatattgg aacccgacag ctttcggaat atggaaaagg tattgggttc
     5461 gacacaaaat ctcataaaga atcatatgat attcgcagca aaagtattgt ggaaaatgca
     5521 ctgaaaaaag cttttgcacc tgagtttctg aatcgtatcg atgatgttat tatgttcaat
     5581 tcacttggaa aatccgaaat ttcgaaaatt attgatatag agctcaaagg ccttttcttg
     5641 cgtgtgaaaa gccttggtta tgatattaaa atttcccctg ccgcaaaaga ttttattgct
     5701 gaaaaaggtt ttgatcctca atttggtgca cgccccctta aacgtgcact tcagaaatat
     5761 atcgaagatg caatggctga atatattctc agatcagagt tgaaatcggg agatacaata
     5821 atggttggat ttaacgaaaa aacaggaatt aaactacgca ttctgaaaaa cgataaaatt
     5881 aaaggatgat tgcgtattta attattgcag agtgttttaa taacggtatt taaactatat
     5941 aattaaccca attgctatga aaaacaagaa actgattctt tggattggtg cagctattct
     6001 gctggttgtg cttactgtag ttgcactaaa aatatttgga aaatcaggta aaaaggagct
     6061 ttctgatcca cagaagtttg aaccttatat cacaggatat accaaaggta ttatatcgaa
     6121 aaacagtgat atagttgttc agtttaccga caagttcatt caggagatta aagataaaaa
     6181 aattacagaa gatatatttg aattcagtcc tgatattaag ggaaagacaa gctgggtcga
     6241 tgaacatacg gttgccttca ccccagctgc tccgcttaag tcgggacaag agttttttgt
     6301 tactgtgaac ttaaaaacgc tgaataagga aattcccaaa gaattcaagg aatttatttt
     6361 taaaacaaaa gttaaaaatc aaaattttga tgtaagcatt tctgagctca tcacaaccga
     6421 tttgaatgat ttcagcaaac aggatgttat tggaactata cgtcttgctg atgtagaaag
     6481 tgaagataag gttaaaagcc tgattagtgc ctctgtagga ggtaaaaaac aaacaatcag
     6541 ctttgaaaaa attgatgact tgcgattttc ttatcgtgtt actggtatta caagaacaaa
     6601 tgctgcacag gagttgataa tatcatatga tggaaaacca attgaagtaa acaaaaacgg
     6661 agttgtaaaa tacccccttc ctgcactcga taatttctct attattgaag ttaaaataaa
     6721 caactttccg gagcaaagcg ttgtaattca gttctctgac cccataatgt cggaacaatt
     6781 gctcgatggc cttattacca ttggtgacga agccagcgga gattttatca tccgcaacaa
     6841 tattattaaa tttattccag cagagcgcat tgaaggcgaa tatgagttgg ttgtaagtga
     6901 aggtgttaag aatattaagg gcaaagaatt cgaagccact tattccaaaa cactgaattt
     6961 tgaatcgctt aaaccacaag tacgattggt aagttcaggg gttgttctgc ctacaatcga
     7021 tgcagggcta gtgttcccat tcgaagccgt taaccttaaa gctgtagatg tgagaattac
     7081 ccgcattttc gaaaataata ttctgcagtt cctacaggtt aataatctgg atggtgactc
     7141 ggaacttgaa agggttggaa aagttgtaaa agaaacaacg gttgacctta gtgccagcgg
     7201 ggttaccgat tttggtgaat ggaatcgttt tactcttgat ttgaacgatt taattaaaac
     7261 tgagcctgga gctatttaca ggattgaaat tggcttccgt aaaaaacact cggcttaccc
     7321 atgcagcgat accatctccg agaatcctgc tactagcgaa gtatatgaca atgattcaac
     7381 tttttgggat tatttcgaaa actacactta tgacgaatat gattattact attatgatta
     7441 ttattacgat gattacgata aacccagccc ttgcaagaat agttattacg gctataaaaa
     7501 tgtggtttca agaaatatca tggcatctga tattggttta acagcaaagt taggcaaaaa
     7561 caatcaggtg gttgctatta caacgaatct gttaacagca aaaccaatga gcggtatcga
     7621 aataaaggtc tatacatttc agatgcaggt tattgctacc ggaaaaacca atgaagacgg
     7681 ggtatcgacc atttcgatac cagcaaaaga aaaaccatac tttgtagttg cctccgatgg
     7741 aaaacaaaag gcatatgttg cattgaatta tggatcgagc ctatctctta gcgagttcaa
     7801 tgtatctgga gcatcctccg gcaattcaaa aggtttcatt tttggagagc gcggagtatg
     7861 gcgccccggt gattctattt atctcagttt tatattgcag gataaggata ttcctattcc
     7921 cgaagggcac cccattgtac ttgaagttac caatcccagc ggtcaactaa tttatcgcga
     7981 agttcaaaat aaaaacacat ggggttatca cacgttcaaa ttcaagacag accaaaagag
     8041 tgtgactggt aattatgctg caaaaattag tgttggaagc gatgtatatt acaaaacctt
     8101 acccgttgaa acaatcaaac ccaaccgact taaaataaac ctggattttg acaaatctta
     8161 tatttcgggt gacggtgaag taaatgccac acttacaacc cagtggctgc atggtgcagt
     8221 tggcaaagat ctgaaagctc aaatcaatat caatcttgat aatgcatata ccggattcga
     8281 taaatttgag ggttacactt tttctgactt cacaaaaaga tttcaattca caacccaact
     8341 gctttttgat ggggtaaccg atgaaaacgg taaagtggtg caaccggtaa aactggacat
     8401 aggaaatcag gcaccagggg ttctaactgc ataccttacg gcaaaagtct tcgaaaaagg
     8461 ggggaatttc agtattttcg aaaaaagcat cgtttaccac ccttattcta attatattgg
     8521 aattaaaccc gatttaaaaa atagctccaa ttatgtaaag ccaggctcta aaaacaagat
     8581 gtctgtggtt atagtcgatc gcaacggaaa agcaataact ggtagtaaag aactggagct
     8641 ggaagtcttt aaatacgact attcttggtg gtatgattat gaaaatacgc agaccaattt
     8701 tgcttcggcc aattacaata atgctttatc aagagaaaag gtaactattt cgaatggcaa
     8761 aggaagcatc caattcgagg ctccggacgt taattatggc agttttattg taattgtttc
     8821 cgataaaaaa ggtggtcact ccgcatcaac cacaatttat gtaagttcct acgattatta
     8881 ttacgatgac ggggaaaatc accaaaacgt aaaccagata atgcttacag caaataaaga
     8941 cacctttgct gttggcgaag atattaggat taatattcct tcggttgatg gcaatgccct
     9001 gatatcaatt gaaaacggtt caagagtctt gaagacgttc tggcaggaaa cacacagggg
     9061 aactaccgag gtagttttta aagctacccg cgaaatgagt ccgaatattt ttatcaacat
     9121 cagcctcttt cagcctcata gcagtaccgg taacgatcat ccaatcagaa tgtacggatt
     9181 aataccggtt atggtggaag accctgaaac tcaccttcat ccggttatca ctatgcccaa
     9241 tgagctccgc tccgaaagta cttatacggt taagatatcg gaaaaagatg gcaaagccat
     9301 gtcgtatacc atagctgttg tggatgaggg aattctcaac ctgaccgggt ttaaaacacc
     9361 tgatccatgg tcgcatttct atagcagaga agcattgggc gtaaacacat gggacatgta
     9421 cgactatgta atcggtgctt tcggtgttga aatagaaagg cttttaagca taggtggaga
     9481 tgccggtctg aaagagcggc taggcgacct gaaagccaat agatttgaac ccatggttcg
     9541 ttttatcgga ccttttcatc tggaaaaggg gaaaacagcg tctcacacta tagctgctcc
     9601 ggcatacgtg ggttctgtaa gaacaatggt tgtagcccgc gaaaacgcgg cttatggatc
     9661 tgcagaaaag acaactcctg ttacaaaacc cctgatgctt ctcggaacac tcccacgagt
     9721 gctcgggcct caagaagaag ttttcctgcc aataagcatc ttctctatgg atagcaacat
     9781 aaaacaagcc agtgttcagg ttaagaccaa cgacttggta atgattgacg gaagtacaag
     9841 ccagactgtg aattttcctc aaaagggtga acagtttatt caattcaagc tcaaggcggg
     9901 taataaaacc ggtgttgcta aaatcatggt cactgcacaa tcgggtacaa acattgctac
     9961 acagcaaatt gaagtagata tacgttaccc gataacaacc cagaccgatg ttaaaagcgg
    10021 tattgctgat gcgggtaaaa gcattgaaga aagcttttca gcgacaggaa ttcaaggaac
    10081 aaataaactt tgtgttgaag tttattcaat tccgccatta aatttggaaa agcgcctgaa
    10141 ttatctaata caatacccat acggctgtat tgagcaaact
//
LOCUS       DAOOQP010000107        10136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8261_length_10136_cov_4.533082, whole genome shotgun sequence.
ACCESSION   DAOOQP010000107 DAOOQP010000000
VERSION     DAOOQP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_10136_cov_4.533082"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLK75_10165"
     CDS             <1..901
                     /locus_tag="PLK75_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOY39651.1"
                     /translation="ALLNEILIDTTTIELKNTSQLYFKPLLLHEALRLRNEFPLATIV
                     NGSTDIALRQTKKKELIRQIIDISDVSELNGFYEDDNQYIFGAGTTIETVLKQSSGKI
                     PALETMLAQFGSLQIRNIATIGGNLSSASPIGDTTPVLFVLGAHVRLKSIISDRQIPV
                     EDFITGYRKTVMDFGEILTEIMIPKYDASYLTASYKVSKRKSLDISTLSAAFLVKLQD
                     GNVEEIRIAFGGMAAVTSFAVKTQSFLNSREWNRKYIEQAIQILSEEFTPISDARADA
                     NYRKNVAANLLLKFFISTPYGAK"
     gene            916..3222
                     /gene="xdhB"
                     /locus_tag="PLK75_10170"
     CDS             916..3222
                     /gene="xdhB"
                     /locus_tag="PLK75_10170"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HOY39652.1"
                     /translation="MNKEIHHDSAELHVTGKSVYVNDIDAGEGMLLGKVLFSKHAHAK
                     ITSIDITGALKIQGVKTIMLAADIPGTNQMGPVVHDELCLADTEVCFIGQAIALIAAI
                     DRDALHAAEMAIHIEYELLEAVVTLDEAIERNTLIAPPRTIVRGNATEAIKNAPNSLV
                     GELHTNGQEHWYLETQSALAVPGENRDMFVYASSQNPSETQAIVAEVLGLPKNKVEVE
                     VKRMGGGFGGKETQGNHVAAWASLLAYATGKPVKMHLFRDDDQIMTGKRHPAYSKYEV
                     GFDNDGKILGYKVKIHINAGAATDLSMAILERAMLHAENSYFIPATEIIGRAYKTNLP
                     SNTAFRGFGGPQGMAVIENAIDRIARFLQKEAAEVRKLNFYGISKNNIAPYGQIIENN
                     RLHQIYDQLIISSEYLGRRRKITDYNMSHEFSKRGMALTPVKFGISFTTSFLNQAGAL
                     VNIYNDGTVLVNHGGTEMGQGLHTKIKQIAALELGIDTERVKVNATNTSKVPNTSPTA
                     ASSGSDINGMAVKNAIDVLKERLCHVAVEEFLTVCPDMKICTGDIIFADNKLVCALCP
                     ALAIPFNEIAAKAHLKQVSLSSTGFYKTPGIYFDRQKGEGKPFYYFSYGMAVSEVEID
                     VLTGQYSLIRTDIINDSGSSINESIDIGQVQGAFIQGVGWVTTEELKWDKKGNLLTHS
                     PDTYKIPAIADIPSDFRVELLKNVPNEGTIRRSKAVGEPPFMLAFSVWLAIKDAIASI
                     CNNQLEPEIRIPMTPEAVLNSITQIKNE"
     gene            3215..3874
                     /locus_tag="PLK75_10175"
     CDS             3215..3874
                     /locus_tag="PLK75_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOY39653.1"
                     /translation="MNRKIKTLIFIVLFGTVMLSNVIAQEVTRIDLPEPVMSGGKPLM
                     DVFKSRCSQRSFSNQELTLQSISNLLWAANGINRPDSKKRTAPTAMNDQEIEVYLANK
                     DGVYVYLPEEHAIELVESGDLRGKFGIQGFVKDAPVILIYVADYKKMSSLMSKEDKDF
                     YAATDVGFVSQNVYLFCASEGLATVVLGMVSRDSAAKSINLDSGKKIMLTQCVGYPLE
                     K"
     gene            complement(3949..4512)
                     /locus_tag="PLK75_10180"
     CDS             complement(3949..4512)
                     /locus_tag="PLK75_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39654.1"
                     /translation="MKSIQNIIERINVRYEELGESFNINQLRSIIRDQETPIEDIEIP
                     DVPGEMYPYSRKIITSTEIVEITVLRWNINGQSMIHDYGESYGIVRVLSGHLNFTLFN
                     SELNEIGQGSVPAIDTFDLPGKLIHKMYNPSNYQETISLHFYCPKLNGINIYLPDENR
                     KIALKGGIGAWYPQTSDIISEGKLHSS"
     gene            complement(4751..5590)
                     /locus_tag="PLK75_10185"
     CDS             complement(4751..5590)
                     /locus_tag="PLK75_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOY39655.1"
                     /translation="MFPSNLHTHTDFSDGTHPPEVYINQAIACGLQTYGFSDHAPLTF
                     RNFGWCLKPEKVNAYCNEIKRLKAEYSNHLNILLGFEVDFIPGVSAPSDVEKYKPDFI
                     IGSIHFLKKPNRDYIMEIDGGYENFREGFKYLFADNPKALAEFYFDTVSKMVQAGGFQ
                     IIGHVDKIKMYLNRCFPGIYQEPWYKNLENEYAIFLGKSEIIVEINTRSIYKKHFTEP
                     YPSWDMIKTIWEAGGKLTLSGDTHDPHGVSAYFKYCSDVLKKMGITHLQAHNGTSWFD
                     YKL"
     gene            complement(5662..9156)
                     /locus_tag="PLK75_10190"
     CDS             complement(5662..9156)
                     /locus_tag="PLK75_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOY39656.1"
                     /translation="MKYRFLLIALIAIPLTGFSQVYSGAAADKLYSGANQIRLKPFTH
                     VPDYISFYEAFRPGAEKGEAVIAAFFINPKLSLKKIEQFGDKLGWEHFRYAQTYDGTD
                     IEFTQYIVHTKNGKVLSMNGSIFSNPVVSGSFIISNDDAVNAALDYTGAKKYMFETEK
                     TELPEDYTSYIKPVAEAVYFPENVELEKNHIIAAYRVDVFSLEPYDRKWVYVNAENGE
                     IITSISRIQTTDVPGTAQTAYSGSQTITTDYTGSYYRLRETGRGNGVSTYNCQLTTSY
                     NSAIDFTDNDNIWNNANANLDQYATDAHFATERTYDYYYNVHGRNSINNSGFHLKSYV
                     HFNLVEAGYSSNVNAFWNGECMTYGDGDETRTPLTTIDICAHEITHGLTSYTANLVYQ
                     NESGALNEAFSDIFGTVVEHYAAPDYFDWTIGEDIAYPFRSLSNPNEYGLPDTYQGLN
                     WHTDPGSDNGGVHRNCGPLSYWFYLISEGGSGTNDLNHNYNISGIGMNDAADISYRLL
                     TVYLTNTSNYADARYYGIQAAIDYFGACSEEVEKVTNSFYAIGVGDAYVPEVIAGFTS
                     DYAENCSAPFTVQFYNTSMNASDFLWNFGDGTTSTEVNPEHTYNSAGNFNVSLSADGG
                     TCGDDNSTITDFIRIGPEYPCLIFMPESGNVATSQCSGILYDIGGPSNPYYDNTFASY
                     TIQPPGASKIILNINEFDIEPGSGSTCNYDYIAFYDGVNTSAPLINNTTYCNTNGNPG
                     TIESTGGAITIAFYSDPGLSLQGFEIEWNCLQDNGSPLAAFSAVPTSSCSGIIQFTNE
                     SLNEPDSFYWTFGDGNNSSDENPTHLYSNDGNYTISLTVSNDFGENTKTKTGYIQVSL
                     AEPPPYSDTIICIDSVFTLGNASGEAIHWYADEGFTDLVHTGATWEHEPISEVTSYWL
                     RSFDNSETYYVGETQSNVGGGPFGNPSYIHYLVFDAYKPFTLVSVEVNADGNGERQIA
                     LRDAQMNTIETRNVYCSNGIQRIDINFNIPVGESLQLVGLNSPNLFRTNESAYLNYPY
                     TIEDVCSIKYSSASQSPTGYYYYFYDWEILTQACESEPKHINITPIECTSSAGTNSIE
                     PDITIYPQPTSEFVHISGLKDTEEYFATIFDASGLEISKKFILSNNRIDLSYFTPGLY
                     IIRIETDSFTIYRKLIKS"
     gene            9316..9774
                     /locus_tag="PLK75_10195"
     CDS             9316..9774
                     /locus_tag="PLK75_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOY39657.1"
                     /translation="MHKEVVYDTVKKMFADYLEKNGHRKTPERFAILDEIYNNNGHFD
                     IENLYISMKNKNYRVSRATLYNTIDLLMKTKLVVRHQFGSNVSYYEKALSNTKHDHMI
                     CVVCGKLTEFSDQRIEDIKNSISENYQFGVSHHSLYIYGTCSNCIQKANK"
     gene            9847..>10136
                     /locus_tag="PLK75_10200"
     CDS             9847..>10136
                     /locus_tag="PLK75_10200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOY39658.1"
                     /translation="MKTDVLLGLQWGDEGKGKIIDVLAENYDIVARFQGGPNAGHTLE
                     FNNIKHVLHTIPSGIFRENKVNIISNEIVLDPISFRNELQTTAKQNGFVYEN"
BASE COUNT         3073 a         1909 c         2106 g         3048 t
ORIGIN      
        1 tgcgttattg aacgaaatat tgattgatac cacaaccatt gagctgaaaa atacttctca
       61 gttgtatttt aaaccactac tcctgcacga ggcattgcgt ttgcgaaatg aattcccctt
      121 ggcgacgatt gtaaacggat cgaccgatat tgcgttgcgc cagaccaaaa aaaaggaact
      181 gatccggcaa ataattgata tttcggatgt gtccgaattg aatggttttt acgaggacga
      241 taatcaatat atattcggag cgggcactac catcgaaacg gtattgaagc agtcatctgg
      301 aaaaattcct gcactcgaaa ccatgctggc gcaatttggc tctcttcaga tacgaaatat
      361 tgctacgatt ggcggtaacc ttagctctgc atcacccata ggagatacaa cacccgtgct
      421 gtttgtactc ggggcacatg ttaggcttaa atctataatt tccgacaggc aaataccggt
      481 cgaagatttt ataaccggct accgaaaaac ggtaatggat tttggagaaa tacttaccga
      541 aataatgatt ccaaaatatg atgcatcgta tttgacagcc tcatataagg tgtcgaaaag
      601 aaaaagtctt gatatttcaa ctctaagtgc agcttttcta gtgaaactac aagacggtaa
      661 tgttgaggaa attcgtattg cttttggggg gatggctgct gttaccagct ttgctgtaaa
      721 aacccagtcg tttttgaata gcagggagtg gaatcgtaaa tatattgagc aggcgataca
      781 aattctgagc gaggagttta ctccaatttc ggatgcacgt gccgatgcca attatagaaa
      841 aaatgttgct gcgaacctgc tgctcaagtt ttttatcagt accccgtatg gtgccaaata
      901 acaaatttct tttttatgaa taaagaaata catcacgaca gtgctgaact ccatgtaaca
      961 ggtaaatcgg tctatgtaaa tgatattgat gcaggtgagg gtatgcttct cggaaaagta
     1021 ttgttcagta agcatgccca tgcaaaaata acaagcattg atattactgg cgctctgaaa
     1081 attcagggtg ttaagactat catgctggct gcggatattc ccggtacaaa tcaaatgggt
     1141 ccggttgttc acgatgaatt gtgccttgcc gacaccgaag tctgttttat cgggcaggct
     1201 atagcattaa ttgctgcaat cgacagggac gcattgcatg cagcggaaat ggcaattcat
     1261 atcgagtatg aattattgga agctgttgtt actttagatg aagctattga aagaaacaca
     1321 cttattgcac caccaagaac cattgtgcgt ggtaatgcaa ctgaagctat aaaaaatgct
     1381 cccaactcac ttgtcggcga attgcataca aacggacagg aacactggta tctcgaaacg
     1441 caatcagcgt tggcagttcc cggagaaaat cgtgatatgt ttgtttatgc atcatctcaa
     1501 aaccccagtg aaacacaggc gattgttgca gaggtattgg gattgccaaa aaacaaggtg
     1561 gaagttgagg taaaacgtat gggtggtggt tttggtggta aagaaacaca gggtaatcac
     1621 gttgctgcat gggcttcgtt gcttgcatat gctacgggta aaccggtaaa aatgcatctt
     1681 ttccgcgacg acgatcagat aatgacagga aaacgccatc cggcatattc gaaatatgag
     1741 gttggattcg acaatgatgg gaaaatactg ggttataaag ttaaaataca tattaatgcc
     1801 ggtgcagcta ccgacctgag catggcaatt ctcgaaaggg ctatgcttca tgccgaaaat
     1861 tcctatttta tcccggccac cgaaattatt ggcagagctt ataaaacgaa tcttccttct
     1921 aatactgcat ttcgcgggtt tgggggacca cagggtatgg ccgttatcga aaatgctatc
     1981 gaccgtattg cacggttcct tcaaaaagaa gctgcagaag tcagaaagct gaatttttat
     2041 ggtattagta agaataacat tgccccatat ggtcaaataa tcgaaaacaa tcgcctgcat
     2101 caaatctacg atcaattgat catttcttcg gaatacttag gacgacgtag aaaaattacc
     2161 gattataaca tgtcgcatga gttttcaaaa agaggtatgg cgcttactcc tgttaagttt
     2221 ggaatatcgt ttacaacatc tttcctgaac caggcgggag ctttagtgaa tatctacaac
     2281 gatggaaccg tgcttgtgaa ccatggagga actgaaatgg gacaggggtt gcacacaaaa
     2341 attaaacaaa ttgcagctct tgagttgggt attgatactg aaagggtaaa agtaaatgcc
     2401 accaatacgt caaaagtacc taacacatcc ccgactgcag catcatcggg aagcgatata
     2461 aacggaatgg cagtgaaaaa tgctattgat gtgttgaaag aaagactttg ccacgttgca
     2521 gtggaggaat ttttaacggt ttgcccggat atgaaaattt gtacaggaga tataattttc
     2581 gcagataata agcttgtttg tgcgctatgc cctgcattgg caattccgtt taatgaaata
     2641 gcagcaaaag ctcatttgaa gcaagtaagt ctcagttcaa cgggttttta taaaacgccg
     2701 ggtatttatt tcgaccgtca aaaaggagaa gggaaaccat tctattattt ttcgtatggc
     2761 atggctgtgt cggaggttga aattgatgta cttacagggc agtattcttt aatacgcacc
     2821 gatattatta acgattccgg tagttcaatt aacgaaagca tcgatattgg tcaggttcag
     2881 ggggctttta ttcagggagt gggttgggtg acaaccgaag agttaaaatg ggacaaaaag
     2941 ggcaacctgc tcactcattc gcccgatacc tataaaatac ctgcaattgc tgacattccc
     3001 agcgattttc gggttgagct tttaaagaat gttccgaatg aaggtacaat ccgtcgcagc
     3061 aaagcagtgg gcgagccgcc cttcatgctt gcattttcgg tttggctggc aattaaagac
     3121 gctattgcct cgatctgcaa taatcagtta gagcccgaaa tacgtatccc aatgacaccc
     3181 gaagctgttt taaactcaat aacacaaata aaaaatgaat agaaaaatta aaaccctgat
     3241 ttttatagta ctgtttggta cggttatgtt aagcaatgta atagctcagg aggtaacccg
     3301 tattgatttg ccagaaccgg ttatgtcggg tggcaaaccc ttgatggatg tttttaaaag
     3361 tcgctgctcg caacgaagtt tttctaatca agaactcaca cttcagagca tttctaattt
     3421 actttgggct gcaaatggta taaatcgccc tgactccaaa aagcgtactg cgcctactgc
     3481 tatgaacgat caggaaatag aagtttacct tgctaataaa gatggagtat atgtctatct
     3541 gcctgaagaa catgctattg agttggttga atcgggcgat ttacggggaa aatttggtat
     3601 tcagggcttt gtaaaggatg ctccagttat attaatttat gttgccgatt acaaaaagat
     3661 gagcagcctg atgtcgaaag aagataaaga tttttatgct gcaaccgacg ttggttttgt
     3721 tagtcaaaat gtgtatttgt tttgtgcttc cgaaggtctg gcaacagttg ttcttgggat
     3781 ggtgtcacgc gatagtgcag caaaatcgat aaatcttgat tccggaaaaa aaattatgct
     3841 gactcaatgc gttgggtacc cattggaaaa ataaatgcgt aactgtttct gtgtaatcga
     3901 ggtaatgtta acgacccaag tcgcgaaaaa tgattgcttg atataacttt aactggaatg
     3961 taatttgcct tccgaaataa tgtcggaagt ttgtggatac caggcaccaa tgcctccttt
     4021 taatgcaatc tttcggtttt cgtcgggcag gtaaatgtta atcccattta gttttgggca
     4081 atagaagtgc aggcttatgg tttcctgata attgcttggg ttgtacattt tatgaataag
     4141 tttaccaggc agatcaaaag tgtcgatagc aggaacgcta ccctgtccaa tttcgttcaa
     4201 ctcagaatta aataaggtga aattcagatg tcccgataat acacgaacaa taccatacga
     4261 ttcaccgtaa tcgtgtatca tgctttgccc gttaatattc catcgcagca cagtaatttc
     4321 aacaatttcg gtgcttgtga ttatttttct ggaataaggg tacatttctc ccggtacatc
     4381 gggtatctca atatcctcta tgggagtttc ctgatcacga ataatagatc gtaactgatt
     4441 tatattgaaa ctctcgccaa gttcctcata tcttacattt atgcgttcaa taatattctg
     4501 aatactcttc ataatgtcca gtttagaaaa tacccttaat tataaccctt ttttttcgga
     4561 aatattgtaa taaagcattt ttactaatct tgtttaatta ccggattcta tttcgaaatt
     4621 attttcatta cggttcaaat gttacatatt tttttgattt atccaaaagt tatacggatt
     4681 aaacagggga acttataact ataatttgaa aggagtcgtt gctatgttgg gtgaattgtt
     4741 ggaaagcgaa ctataattta taatcgaacc atgaggtacc attgtgagct tggagatggg
     4801 tgattcccat tttttttaaa acatcagaac aatatttaaa gtaagcgcta acaccatgag
     4861 ggtcgtgggt atcaccgctc aaagtcagtt tccctccagc ttcccatata gttttaatca
     4921 tatcccagga gggataaggt tctgtaaaat gtttcttgta aattgaacgg gtgtttatct
     4981 caacaataat ttctgatttt ccaagaaata ttgcatattc attttccagg tttttatacc
     5041 atggctcctg ataaattccc ggaaagcacc ggttcaggta cattttaatt ttatcgacat
     5101 ggccaattat ttgaaaccct cctgcctgaa ccattttgct aacagtatcg aaataaaact
     5161 ctgccagtgc ttttgggtta tctgcaaaaa gatacttgaa tccttccctg aagttttcgt
     5221 aacccccgtc aatttccatg atataatcgc gattgggctt ttttagaaaa tgaattgagc
     5281 cgattataaa atcaggtttg tatttttcaa catccgatgg cgcggaaacg ccaggtatga
     5341 aatctacttc aaatcccaat aaaatattca gatggttgga gtattctgct tttaaccttt
     5401 taatttcatt acaatatgca tttacttttt caggtttcag gcaccagcca aagtttctaa
     5461 atgtcagcgg ggcatgatca gaaaagccgt aagtttgcaa tccacaggca attgcttgat
     5521 taatataaac ctcgggagga tgtgtcccat cgctgaaatc ggtgtgtgta tgcaaattcg
     5581 atggaaacat aaacgccgtg tttattatca aaataagcgt atttcgatga acaaaacaag
     5641 cattatgtta ttaaatagct tttacgattt aataagtttc cggtagattg tgaatgagtc
     5701 agtttcaatt ctgatgatgt acaagcccgg agtaaagtag cttaaatcaa tgcgattatt
     5761 gctgagtatg aattttttag aaatttccaa accgcttgca tcaaaaatgg tcgcgaagta
     5821 ttcttcggta tctttcaatc ccgaaatatg aacgaattct gatgtaggtt ggggataaat
     5881 tgtaatatcg ggctcaattg aatttgttcc tgcagaagaa gtgcactcta ttggagtgat
     5941 gttaatatgc ttgggttcac tttcacatgc ctgagttaaa atttcccaat cgtaaaagta
     6001 atagtagtag ccagtggggc tctgcgaagc actgctatat ttaattgaac aaacatcttc
     6061 gattgtgtaa ggatagttca gataggcact ttcattagtt ctgaaaagat ttggagaatt
     6121 aagtccgaca agctgcagcg actctcctac aggaatattg aaattaatat caattctttg
     6181 aatgccattg gaacaatata catttctggt ttcaatggta ttcatttgtg catcacgaag
     6241 agcaatttgt ctttctccat tcccgtcagc gtttacttcg actgaaacaa gggtaaatgg
     6301 tttataagca tcgaacacca gataatgaat atacgaagga ttgccaaaag gacctccgcc
     6361 tacattgctt tgcgtttcac ccacataata ggtttctgag ttatcgaagc tacgaagcca
     6421 gtaagaggtt acttcggaaa taggttcatg ttcccatgtt gctccggtat gaaccaagtc
     6481 agtaaagcct tcatcagcat accaatgtat tgcttctccc gaggcattcc ctaaggtaaa
     6541 aacggagtcg atgcaaataa tcgtatcgct atatggcggt ggttcagcca gactaacctg
     6601 aatatagcct gtttttgtct ttgtgttttc accgaaatcg ttcgaaacag ttaaactaat
     6661 tgtgtagttg ccgtcgttgc tgtacaaatg agtcggattt tcatcagacg agttgttccc
     6721 atcaccaaaa gtccagtaaa atgaatccgg ttcattgagc gattcattgg taaactgaat
     6781 aattccgctg catgatgatg ttggtactgc agaaaatgca gccagcgggc tgccattgtc
     6841 ctgaaggcaa ttccattcga tttcaaagcc ctgtaggcta agaccagggt cagagtagaa
     6901 agcaatggta attgccccac cggttgattc aattgttcct gggtttccgt tggtgttgca
     6961 atacgttgta ttatttatca atggtgccga agtgtttact ccgtcgtaaa atgctatata
     7021 atcgtagttg catgtgctcc ctgaaccggg ttcgatatca aattcattga tattcaggat
     7081 aattttagaa gcacccggag gttgaatggt gtaacttgca aatgtattgt cgtagtatgg
     7141 attggaaggt cccccaatat catatagtat tccggagcat tgactagtag ctacattccc
     7201 gctttcaggc atgaaaatca aacaaggata ctccggtcct atccgaataa agtcggtaat
     7261 ggtggaatta tcatcaccac aggtaccccc gtcagctgac agacttacgt taaagttacc
     7321 agcgctattg tatgtgtgtt ctggattgac ttcagtactg gtagtgccat ccccgaaatt
     7381 ccacagaaaa tcgcttgcat tcatgctggt attataaaac tgaactgtga atggcgctga
     7441 acagttttca gcgtaatcgg atgtgaagcc ggcaattacc tccggaacgt aggcgtcgcc
     7501 aacaccaatt gcgtaaaaag aatttgtaac cttttcaact tcctcagagc atgcgccgaa
     7561 atagtcgata gctgcctgaa tcccataata acgtgcgtct gcataattag aggtattggt
     7621 caggtaaaca gtcagcaatc ggtatgaaat gtctgcagca tcattcatgc caatacctga
     7681 aatattataa ttatgattta ggtcatttgt accagagccc ccttccgata ttaagtaaaa
     7741 ccaatacgat aaaggtccgc aattccgatg tacaccgcca ttatcgctgc caggatcagt
     7801 gtgccagttt aggccctgat aagtatcggg taaaccgtat tcattaggat tggaaagtga
     7861 ccggaagggg taagcaatat cctctccaat tgtccagtcg aaataatcag gggcagcata
     7921 gtgttcaact acggtgccaa aaatgtcgct gaaggcctcg tttagagctc ctgattcatt
     7981 ctgatatacc agatttgcag tataggaggt caatccatgg gttatttcat gagcgcatat
     8041 atctatggtt gttagcgggg tccgggtttc atctccatct ccgtaagtca tacattcacc
     8101 attccagaat gcattcacat tacttgaata ccctgcttcc acaagattaa aatgtacata
     8161 tgatttcaga tgaaatccgg agttgttgat gctgttgcga ccatgaacat tgtagtagta
     8221 atcgtacgtt ctttctgttg caaaatgagc atcagtggca tactgatcca gattcgcatt
     8281 cgcattattc caaatgttat cattgtcggt aaaatcaatc gcggaattat aactggtggt
     8341 taactggcag ttgtaagtac ttacaccatt accccttccg gtttcgcgca gcctgtaata
     8401 actgccggtg taatcagtcg taattgtctg acttccactg taagcggtct gtgcagttcc
     8461 gggaacatcg gtagtttgta tacgtgatat actggtaatg atttctccgt tttcagcatt
     8521 tacataaacc cattttctgt cgtagggctc caatgaaaat acatcaaccc ggtaagcggc
     8581 aataatgtgg ttcttttcca gttcaacatt ttcagggaaa taaacagctt cagcaaccgg
     8641 ttttatgtaa cttgtataat cttcaggcaa ctctgtcttt tcggtttcga acatatattt
     8701 ttttgcgcct gtataatcaa gtgcagcatt tactgcatcg tcattgctta taataaaact
     8761 tccacttaca acaggatttg agaaaatact gccattcatc gaaagcactt tcccattttt
     8821 ggtatgaaca atatattgtg tgaattcaat atcggttccg tcgtatgtct gtgcatacct
     8881 gaagtgctcc caacccagct tgtcgccgaa ctgctctatt ttttttaaac ttaacttggg
     8941 gttgataaaa aatgctgcaa ttactgcctc tcctttctcg gcgccaggcc tgaatgcttc
     9001 atagaacgag atatagtcgg gaacatgcgt aaatggcttt aagcgtatct gattggcacc
     9061 tgaatacaat ttatcggcag ccgcaccact ataaacctgt gaaaatcctg taagtggaat
     9121 tgctattagt gcaattaata aaaaacggta cttcataatc aatgttatta aaaaaaagca
     9181 agataaacaa tattccttta aaagatgtat cgtgaatata aaactcaaaa cactttgccg
     9241 aaaataaaac ggaattttct gaaaaacttt gtttttttaa aaaattattt taatttagac
     9301 ttaaatttaa taacgatgca taaagaagta gtatatgata cggtaaagaa gatgtttgct
     9361 gattatcttg aaaaaaacgg acatagaaaa acccctgaac gttttgcaat tcttgatgaa
     9421 atctacaata ataacggaca cttcgacata gagaatttgt atatctcgat gaagaacaag
     9481 aactatcggg taagccgtgc aaccttgtac aataccattg atttactgat gaaaacaaag
     9541 cttgtcgtca ggcatcagtt cggaagtaat gttagttact acgagaaagc actttccaat
     9601 acaaaacacg accacatgat ttgcgttgta tgtggaaagc tgacagagtt ttctgatcag
     9661 cggattgagg acattaagaa ttctatttcc gaaaattatc aattcggggt ttcgcatcat
     9721 agtctttata tatacggcac atgctcgaat tgcatacaaa aggccaataa ataatgatct
     9781 cttacatgtg ttttaacata tttattttga gcttaatgtt ctatctttgc taattatatt
     9841 ttttttatga aaacagatgt tttgttagga ttgcagtggg gcgatgaggg taaaggaaaa
     9901 attattgatg ttctagccga aaactacgat attgttgccc gttttcaggg aggacccaat
     9961 gcaggtcata ctcttgaatt taacaatata aagcatgtat tgcatacaat tccctcgggt
    10021 atcttcaggg aaaataaggt aaatatcatt agcaatgaga tagtactcga cccgataagt
    10081 ttcaggaatg aattacaaac aacagctaaa caaaatggat ttgtttacga aaactt
//
LOCUS       DAOOQP010000108        10064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8315_length_10064_cov_5.509242, whole genome shotgun sequence.
ACCESSION   DAOOQP010000108 DAOOQP010000000
VERSION     DAOOQP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8315_length_10064_cov_5.509242"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLK75_10205"
     CDS             <1..236
                     /locus_tag="PLK75_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39659.1"
                     /translation="NENIYAGLIEMLQNDSIAVINLYQYYSDPEIKTQIAAYFWREGH
                     HNPRGYKLMAEGVYRGLVINKVLPEDSADKQLR"
     gene            complement(272..1849)
                     /locus_tag="PLK75_10210"
     CDS             complement(272..1849)
                     /locus_tag="PLK75_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOY39660.1"
                     /translation="MKVALIQCPAWNTDSPPHGLSLLSADLKNHGHSVMCYDLNMAIF
                     RCALSQSGLPEQKEWDFTRIDDNWTDLNVVQQVFEKSLPCLSPYIDKIREYNPEIIGF
                     SVFSTTAYFSYFLTRHFRIEFPDAKIIWGGPECYENFGRVEYIAKHIPEIDVIFTGYS
                     CGIAPAVVDFFEKYSVFPASAPGYLNTNVNSNTSFISADTDFNQTPVADFRAFNVADY
                     AMQYFPVWVNKGCVNRCAFCQECRANTTYEWRDPGIIAKEIAHYKTELPQIQHFWLTS
                     SNIGGNLNKLIELSELLMNAAPGISWVSQISVHMQLTLPVLELMKDSGCNCLYFGIES
                     GSNKVLRLMNKPITRKLIKQVVRNTVKVGISFNFNLIVGFPGETISDFIKTLFLIHHF
                     RRYGISPSVATCKIIPNSTLFMQPEKYGIINHTQDNWLTEDRKNTLQTRNKRKQIAER
                     AFSGSLINMFQIPYRLFLFAQYYGNNKASGVKYSLVIPANILSKLLFMISFIPFVIWI
                     SVISIRLKYNELRLINT"
     gene            complement(1855..3282)
                     /locus_tag="PLK75_10215"
     CDS             complement(1855..3282)
                     /locus_tag="PLK75_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39661.1"
                     /translation="MTPIEPINLNTAQTLYKTVLLNPPGDKMILRDYYCAKVSKARYY
                     YHPVDFIYLSGRLAALGELMVIDAIASQLSAEETLDKISRFEPSAVIFLSSAPSFVAD
                     MAFMQRLSVQLPHTQIIGTGDVFRDYGKEAMIRYPFMHAVFIDFSTDDIIRTLTHQSG
                     VINNVIYRKNNELIAGTELHGNGVFDVPLPRWDLFDIAAYRFPFARRKPFASILTDFG
                     CPFSCDFCPVSSLGFKLRSIDSVIEEMTLLSQMGVKELYIRDQTFGANRKRTVALLNA
                     MLDKSLKFSWTGLSRADILDVELVKLMKKAGCHTLMIGIESANDNLMAVHKKNTRVSE
                     VSEKISQIRSTGVRVGGFFMIGFPGESKESVLNTIRLALSLKVDYASFNIATPRLGTH
                     FRRHAIEQGLVQSENIDAESSSAMPVWQNQQLSNNELFNLRRMAVRKFYLRPLYLLKR
                     FAGIRTLNELSNLINEGFALLSKTR"
     gene            complement(3269..4258)
                     /locus_tag="PLK75_10220"
     CDS             complement(3269..4258)
                     /locus_tag="PLK75_10220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY39662.1"
                     /translation="MEANESVDVSVIVPVYNEQGNLKELFNRLQMSLLSIDKTSEIVF
                     VDDGSTDDSFKVLQSLETTQICAKLIRFRRNFGQTAAMAAGLAHSKGKIVVFIDADLQ
                     NDPYDIPALLSKLDEGYDVVSGWRQSRKDTLLFKKIPSRIANAMIRKTSKVNIHDLGC
                     SLKAYRREIIEEISMVGEMHRFLPVLAARIGARITEIPVQHHPRTYGRSNYGLKRSYK
                     VILDLMTVQFFHSFSTKPIHLYGGFALISLLMGLCSGIAVILMKIFWGTDMTGNPILL
                     LTVLFILITIILALLGIQSEILVRIYHQQGRAKQYYIRETHDLTCQGNTDDSN"
     gene            complement(4457..6601)
                     /locus_tag="PLK75_10225"
     CDS             complement(4457..6601)
                     /locus_tag="PLK75_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39663.1"
                     /translation="MKKKLPQKNLTWLTLLFIVNLLVAGGLYIYHLRVNFNSEIKLYS
                     HELRELRVTTPAGGNYTIQVPASDTVVLYARPIHSFAWLHNGSKVIWGDPQQSEEFES
                     LTGISVQIANPDKPSLGMTIRFFTILRMHWPVIMLNIVLLMVWLTRKRSAVKAFCRRI
                     KIRLFRTAIRHKPIGNAGVPPHSRFGIVLLLLALYYVVSLFAFGNRTEIDQISTERIR
                     MLVSLEMNVSGNYFTPTINGKPYYNKPPLFNQLLIPVVDDESDPEYKARLITTFSILV
                     LGFIVFLIARKNLNKEHSLLATLLFLSSFAMCIGYPDWVGLDPLFSAFVLAGVFSNYH
                     FAAKRQYVSMYTIGYLFTVAAFLTKGLPALWFQLASLFFALMVFGKLKVLFSWKHGVG
                     IAVFLLLTGSYFAMSYPLPELFTYFRQLFIGEPELQLNVSMLNRVSNMLSFAGKAMLA
                     YAPVMLLFPAVFLKPGYYSILKSPFQTYLFMMIVSICVVFIPGNFQPHYILCAVPLAA
                     IMLTGNLSMINAMNMRQRGFVTLFFCVAVPLEVFLHQFAYDYFSISYKLYAVSAIAVT
                     LCVALSVFFVKKIRTLLVCFIFTTITLRVIVVGCIPGNVFKKDTYPVKEKAKEIVEHT
                     KGKSLFIYPENTEINHATLYYLTYFYGKIIYKTNDLCRKNAFFLSPSSDIPEGFTIID
                     SISQRQQIRPGEADSVFVCNNKLYLHTVELEK"
     gene            6918..9050
                     /locus_tag="PLK75_10230"
     CDS             6918..9050
                     /locus_tag="PLK75_10230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY39664.1"
                     /translation="MPKLLKIALILIIAFHGLLIAVFCFYYFNTGYTGSFMITSEKMH
                     SITLITPLGEEIECIQAAGDTLITPDRPIRNVFIADENGVSVSFHRNDVNGTLTDNSG
                     LPVYVSGTENGNNRAMAVFSFLMEATPVLKAYIFLWGLVLLAIAIGILLVRRFRVLMK
                     HRVFRWLVKAGKVQRRPELSQPKFDMFRKFVIVAACILLLPATYLRLGKYPFSQSSEE
                     RKRALVSLEMKLQNSYIAPTLNGDAYYNKPPLFNWFLLPVMGCDNVEFATRAVSATFL
                     LLSGVLVFVLLRRQRGGWYAASVALLFMASYHVIMDLSLLLNLDALFSLLIIPLFYLN
                     YRFADKRKFYQLFITGYLLTSLAFMTKGFPALWYQGVSLFGALAFTRNLKMLFSKQHL
                     VGLLTLICIPGLYYIIYSQTADGGRYIARLFYETQIVADYRLTEIVKHFIAFPSQNMI
                     AFFPMALLAPVLLLRRNISLILRNREQGYLLFVVITGSLTFAISPYYLPYYCMIFVPL
                     VIDILLNSLIVNGNMSIKEKMAVTAWMVLFLVGWLVLRQFSHIEYAMYVLLLLFTLLL
                     LNRYRLHIVLSMALIMVLIKLLFPVHTLYADYFGYPTREECRAVVANNSEKPVCVLKG
                     SEKVNYTTVFYLSYFSNRVIRIAPVAAETANVIFISEAAKVPAGAQVIGIIHQSYWTS
                     DASSRLKGKNSDLPVMVFTMREKTSEVP"
     gene            complement(9154..>10064)
                     /locus_tag="PLK75_10235"
     CDS             complement(9154..>10064)
                     /locus_tag="PLK75_10235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39665.1"
                     /translation="GEPFHISKYNGMRNGSPPPKARLLRFGGGEPFLIPLYFEMWDAI
                     LRENKKANIYVQTNGTIINDRIRKWLASGQFTLGVSLDSLNAERFEMIRKNARLSEVL
                     HNIDEFISLMPVRKNISNLVISATATRLNINDMPTLLRYANSNNAQIVFNTVWSPGEM
                     AVYNLPANDLRIIADELSAEVLQEHTLLMKHNASLFRAFLQQMEQWHSLAKLREAKLK
                     EYNASDTSSLEKELRIRLENAMQQNQEGIVKIGLIFDRLRQHIRYRDFLMGVLLEDTG
                     DIVRYLINHNEEEIIETVVSRIEAIP"
BASE COUNT         2837 a         2088 c         2142 g         2997 t
ORIGIN      
        1 aaaacgaaaa catatatgca gggctaattg aaatgctgca aaacgatagt atagctgtaa
       61 ttaatctata tcagtattat tctgaccctg agattaaaac tcaaatagca gcttacttct
      121 ggcgcgaagg gcatcataac cccagaggtt acaaacttat ggctgaaggt gtgtacaggg
      181 ggttggttat aaacaaggtt cttccggaag attcggcaga caaacaattg aggtaaaaaa
      241 aatgaaacag cacatttatc cgctgttggt gttaagtgtt tattaaacgc aactcgttgt
      301 atttcagtct gattgaaata acagatatcc aaataacaaa gggtatgaac gaaatcataa
      361 ataacagttt ggataatata tttgccggaa tcactaaaga gtacttcaca ccgcttgctt
      421 tgttattacc gtaatattga gcaaataaaa acaacctgta gggtatttga aacatattga
      481 tgaggctgcc tgaaaaagct ctctctgcaa tttgttttcg tttgtttcgt gtttgcagtg
      541 tgttttttct atcctcagta agccagttat cctgcgtgtg gttaataatt ccgtattttt
      601 cgggttgcat aaacagggtt gaattcggaa ttattttgca tgttgctacc gaaggagaaa
      661 tgccatacct tctgaaatgg tggatcagaa agagggtttt aataaaatcg cttatcgttt
      721 ctccgggaaa acctacaatc aggttgaagt taaatgaaat acctactttt accgtatttc
      781 ttaccacttg tttaattaat ttccgggtta tgggtttgtt cataagccgg agtactttgt
      841 tgctacccga ttcaatgcca aaatacaaac aattgcagcc cgaatctttc atcagctcaa
      901 gaactggtaa tgtaagctgc atgtgaaccg atatctgcga aacccacgaa atacccggag
      961 ctgcattcat gagtaattcc gacaattcaa tcagtttatt caggttgccg ccaatgttgc
     1021 tcgatgttaa ccagaaatgc tgtatttgtg gtagctcagt tttataatgt gcaatttcct
     1081 ttgcaattat tccggggtct cgccattcgt atgttgtatt tgccctgcat tcctgacaaa
     1141 aagcacaacg atttacacaa cctttgttca cccaaacagg gaagtattgc atggcataat
     1201 cagctacatt aaatgcacga aaatcggcca cgggggtttg gttgaaatcg gtatctgccg
     1261 atataaaact ggtattactg ttgacgttgg tgtttaaata cccgggagca gatgctggaa
     1321 aaaccgaata tttttcgaag aaatctacta cagcaggtgc aattccacaa ctgtagccgg
     1381 taaaaatcac atctatttcg ggtatatgtt tggcaatata ttcaacacgc ccaaagttct
     1441 cgtagcactc cggtcctccc caaatgattt ttgcatccgg aaactcaatc ctgaagtgtc
     1501 ttgtaagaaa atatgagaaa taggcagtag ttgaaaaaac cgaaaaaccg ataatttcag
     1561 gattatattc cctgattttg tcaatatagg gtgaaaggca gggcaggctt ttttcgaaaa
     1621 cttgttgtac aacattcaaa tcggtccaat tgtcgtcgat acgggtaaaa tcccattctt
     1681 tctgttccgg taatccggat tgtgaaaggg cacacctaaa aatagccata tttaaatcgt
     1741 aacacatcac tgaatgtcca tgatttttca ggtcggctga aagtaggctc aacccgtggg
     1801 gagggctgtc ggtattccat gcagggcatt gtatcagggc aactttcatg gcaatcagcg
     1861 ggttttcgaa agcagtgcaa agccttcgtt gataagattg ctcagttcgt taagggtgcg
     1921 aataccggca aatcgtttca gcaagtataa aggccgcagg tagaattttc gaactgccat
     1981 gcgccggaga ttgaacaatt cgttattcga aagctgttga ttttgccaga ccggcattgc
     2041 tgacgacgac tccgcatcga tgttttccga ttgcaccagc ccttgctcga tggcatgcct
     2101 gcggaaatgg gttcccaaac gcggggtggc gatgttgaac gatgcatagt caactttcag
     2161 gctaagggca agcctgatag tattaagcac cgactctttg ctctcacccg ggaaaccgat
     2221 cataaaaaaa ccaccgaccc gcacacccgt gcttcttatt tgagatatct tctccgatac
     2281 ttccgaaacc cgtgtgtttt tcttatgcac tgccatcagg ttatcgtttg ccgactcaat
     2341 gccaatcatc agggtatggc agcccgcttt tttcattaac tttaccagtt ccacatccag
     2401 aatatcggct cgcgaaagac ctgtccacga aaacttcagc gacttatcga gcatcgcatt
     2461 aagcagtgct accgtgcgct tgcggttggc accgaaagtc tggtcgcgta tatataactc
     2521 tttcactccc atttggctga gcaaagtcat ttcttcaatc acagaatcaa tgcttctgag
     2581 tttaaaaccc agactgctta cggggcaaaa atcgcacgaa aacggacatc caaaatcggt
     2641 caaaatgctg gcaaagggct tccgcctggc aaatgggaac cggtatgcag ctatatcgaa
     2701 caaatcccat cgtggtagcg gtacatcgaa gacaccattt ccatgaagtt ctgtaccggc
     2761 aataagctca ttattttttc ggtaaatcac attatttata actccggatt gatgtgtaag
     2821 ggtacggatg atatcatcgg tggaaaaatc gataaacaca gcatgcataa aagggtagcg
     2881 gatcatcgct tcttttccgt aatccctgaa aacatcgcct gtacctatga tttgtgtatg
     2941 tggaagctgt accgatagcc tctgcatgaa tgccatgtcg gcaacaaacg atggcgcgga
     3001 actgagaaaa ataacggctg atggttcgaa acgcgaaatt ttatccagcg tctcctctgc
     3061 actcagctgc gaagcaatgg catcaatcac cataagttca cccagcgctg ccagtcttcc
     3121 gctcaggtaa ataaaatcga caggatgata atagtatcgg gctttcgaaa ccttggcaca
     3181 atagtagtcg cgaagaatca ttttatcgcc gggagggttc agcaataccg ttttatacag
     3241 cgtttgtgca gtattcaggt ttataggctc aattggagtc atcggtattt ccctgacacg
     3301 tcaaatcatg tgtttctctt atgtaatact gttttgctct gccttgctgg tggtagatgc
     3361 gtaccagtat ctcgctctgt attcccagca gcgcgagtat tatggttatc aatataaaca
     3421 gtacggtaag caacaatatt gggttgcccg tcatatctgt accccagaaa attttcatca
     3481 gaattaccgc aatgccggaa caaagtccca tgagcagact tatcagagca aaaccgccat
     3541 ataaatgaat gggcttggta ctgaaagaat gaaagaattg cactgtcata aggtcgagaa
     3601 ttactttata agatcgtttt aatccgtaat tcgaccgacc ataggtgcgt gggtgatgct
     3661 gcaccggtat ttcggtaatt ctggctccta tgcgtgcagc aagcaccgga aggaacctgt
     3721 gcatttcgcc aaccatactg atttcttcaa ttatttccct gcggtaggct ttcaacgaac
     3781 agcccagatc gtgaatattt accttactag ttttgcgtat cattgcattg gcaatacgcg
     3841 aaggaatttt tttgaacaac aaagtgtctt ttcgcgattg cctccaaccg ctcactacat
     3901 cgtacccctc atccagcttg ctgagaagtg ccgggatatc gtacggatcg ttctgcagat
     3961 cggcatcaat aaaaactaca atcttcccct tggagtgagc caggccggcg gccatggctg
     4021 ctgtctgacc aaaattgcga cggaaacgaa taagttttgc acaaatttgg gtggtttcga
     4081 gcgactgcaa taccttaaac gagtcatcag tgcttccgtc atcaacaaaa acaatctcgg
     4141 aagttttatc gattgacaac aaactcatct gaagccggtt aaataattcc ttcagattcc
     4201 cctgttcatt gtaaaccggc acaattaccg atacgtccac cgattcgttg gcttccattg
     4261 tagtatgttt acacaagcaa tttgatgtaa atatacaaaa gaattgtatt gattgtatgt
     4321 tcttgacttt taacttcaga tgataaatga tgtgagattt attaatctgc tataaaaggt
     4381 gataaacaaa atttaatcca tgtcaggtat tttttttgat ggctggacac catgttcttt
     4441 tcctgattgc caattgttat ttttctaatt ctactgtatg caggtatagt ttattgttgc
     4501 atacaaacac actatcggct tcgccagggc gtatttgttg ccgctgactg atactgtcga
     4561 ttattgtgaa cccttccgga atgtcggatg acggtgaaag aaagaatgca tttttacggc
     4621 acaaatcatt ggttttgtaa attattttac cataaaaata cgtcagatag tacagcgttg
     4681 catggttaat ctcagtgttt tcggggtata taaataatga tttccctttg gtgtgctcca
     4741 caatttcttt tgctttttcc ttaacagggt aggtgtcttt tttgaaaacg tttccgggta
     4801 tgcagcccac cacgattaca cgcaatgtta tggtagtaaa tatgaagcat acgagtagag
     4861 tacggatttt tttaacaaag aaaacggata aagccacgca caatgttact gcaatggctg
     4921 atactgcgta gagtttgtaa gaaattgaga aataatcgta cgcaaactga tgcagaaata
     4981 cctcaagagg aactgccacg caaaaaaaca gggtaacgaa cccgcgctgc ctcatattca
     5041 ttgcgttaat catggacaaa tttcccgtca gcattatagc tgcaagtggg acagcgcaga
     5101 gtatgtagtg tggctggaaa tttccgggaa taaaaacaac gcaaatcgac acaatcatca
     5161 taaacaggta agtttggaac gggcttttca ggatactgta atagcccggt ttaagaaata
     5221 cagccgggaa aagtagcata accggagcat aggcaagcat ggctttacct gcgaaactaa
     5281 gcatattgct taccctgttg agcatactta cgttaagctg taattcaggc tctccgataa
     5341 acagttgtct gaaatacgtg aataactcag gcaatggata actcattgcg aaatacgacc
     5401 ctgtaagtaa aagaaacact gcaataccga ctccatgttt ccacgaaaac aggactttca
     5461 gtttcccaaa aaccattagg gcaaaaaaca gcgatgccag ttgaaaccac aaggcaggca
     5521 gtcctttggt gagaaatgca gccacggtga acaaatatcc aattgtgtac atgcttacat
     5581 actgcctttt cgccgcaaaa tgataattgc taaagacccc tgcgagcaca aatgcactga
     5641 ataagggatc gagccctacc cagtcggggt agccgataca cattgcgaaa gaagagagaa
     5701 acaagagtgt ggcaagcagc gagtgttcct tgttaaggtt ttttcgcgca ataagaaaaa
     5761 cgataaatcc cagtaccagt atcgaaaatg tagttatcaa cctagcttta tattcaggat
     5821 cgctttcatc gtcaacgaca ggaataagca actggttgaa tagcgggggt ttattgtagt
     5881 aaggcttccc atttattgta ggagtgaaat aattgcctga tacgttcatc tcgagcgata
     5941 caagcattct gattctttcc gtactgattt ggtcgatttc agtgcggttg ccaaaagcaa
     6001 aaaggctgac aacatagtac agagccaata atagtaagac tatcccaaaa cgggaatgag
     6061 ggggaacacc tgcgttgcca attggtttgt gtcgtatcgc tgttctgaaa aggcggattt
     6121 ttatacgtct gcaaaaagct ttaacagccg acctctttct agtaagccat accatcaaca
     6181 atacaatatt cagcattata acaggccaat gcatgcgaag gatggtaaaa aaccggatgg
     6241 tcattccgag ggaaggtttg tcaggatttg ctatttgtac actaatgcct gtaagactct
     6301 caaactcttc cgattgttga gggtcgcccc atattacttt gctaccgttg tgtaaccatg
     6361 caaacgaatg tattggccgc gcatataata ccaccgtatc actggcgggc acctgtattg
     6421 tataattgcc tccagcaggg gttgtaaccc gtaattcgcg cagctcatgt gagtacagtt
     6481 tgatttcgct gttgaagttt acgcgcaaat gatagatgta taatccgcct gctaccaaaa
     6541 ggtttacgat aaataagagt gtcaaccagg taagattctt ttgcggaagt ttcttcttca
     6601 tattgttttt gcaattactg cttcatgttt cgtaaaatta ataacttatt tagtataatt
     6661 caatgctgcc ccatttgatt gtaaattttg cagcacgaaa aaacactttt aatcttttag
     6721 catgtgcaga ttctttccaa atatgccaaa atgtgcattt atcaacaccc gattcatgtt
     6781 catttgtgat ttttttatcg tttgctacca aaatcaggac agcagagtag ggaaaattta
     6841 tttaaatgca aaaatttaat gttaatttta aagtttgtat tcgttattca gaaactacaa
     6901 tgtcccgttg attgaatatg ccgaagttgc ttaaaatagc cctgatcctt ataatagctt
     6961 tccatgggtt gcttattgcg gtattttgtt tttactattt taataccggt tatacaggta
     7021 gtttcatgat tacctccgaa aaaatgcaca gcattaccct cataacccct ttgggtgaag
     7081 aaatagagtg tattcaggct gccggcgata ccctgattac ccctgacagg cccatacgaa
     7141 atgtatttat tgctgatgaa aacggagtct ctgtaagctt tcatagaaat gatgtaaacg
     7201 gcacactgac cgataattcg ggtctccctg tgtacgtttc gggtaccgaa aacggcaaca
     7261 atcgagcaat ggcagtgttc tcttttctga tggaagcgac cccggtttta aaagcgtata
     7321 tctttctgtg gggattggtg ctactggcta ttgcgattgg aatattgttg gtacgcagat
     7381 tcagggtttt aatgaaacac cgtgttttca ggtggttagt taaagcaggt aaagtgcaac
     7441 gaaggcctga attgtcgcaa ccaaaattcg atatgtttcg gaaatttgtt attgttgccg
     7501 catgtattct gttattgcct gctacttacc tgcgactggg aaagtatcca ttctcacaaa
     7561 gctccgaaga gaggaaacgg gcactggtaa gcctcgaaat gaaattgcag aatagctaca
     7621 ttgcaccaac cctgaatggc gatgcatatt acaacaagcc tccgctattc aactggtttc
     7681 tgttgccggt aatgggctgc gataatgttg aatttgccac ccgggcggtg tcggctactt
     7741 ttctcctgct ttccggtgta ttggtttttg tattgctcag gcggcagcgg ggtggctggt
     7801 atgcagcctc tgttgccttg ctgtttatgg cttcgtatca tgtgattatg gatctgtcgt
     7861 tacttctgaa cctggatgcc ttgttttcgc tgcttattat tcctttgttt tatctgaatt
     7921 accgttttgc tgataaacgc aaattttatc aattatttat aacaggctat ttgttaacat
     7981 cactggcatt tatgaccaag ggctttccgg ctttatggta tcagggagta tcgcttttcg
     8041 gtgcattagc ctttacgaga aacctgaaaa tgcttttcag taaacaacat ctcgttggat
     8101 tgctcacttt aatttgtatt cccgggttat attacataat atactcacaa acggcggatg
     8161 gtggcaggta tatagcacgg ttgttttacg aaacacaaat tgtagctgat taccgtctta
     8221 ccgaaattgt aaaacatttc atcgctttcc cgtcgcaaaa catgattgcc ttttttccta
     8281 tggcattact ggctcctgtg ttgctcctcc gcaggaatat ttctcttatt cttcgaaacc
     8341 gggagcaagg ttacttattg tttgttgtaa ttaccggaag ccttaccttt gccataagcc
     8401 cgtattattt accctattac tgtatgatat ttgttcctct tgttatcgat atattactga
     8461 actcattgat tgtaaacggc aacatgagca taaaggaaaa aatggctgta acagcatgga
     8521 tggtgctgtt tttggtagga tggctggtat tacgccaatt cagccatata gaatatgcaa
     8581 tgtatgtgtt gctgctactt tttacgctgc tgctcctaaa ccgttaccgg ctgcatattg
     8641 tactgtcgat ggctctgata atggtactta taaagctctt atttccggta catactttat
     8701 atgctgacta ttttggctat cccacccggg aggaatgtcg cgcagttgtt gctaataaca
     8761 gcgaaaaacc ggtttgtgtg cttaagggta gcgaaaaagt taattatact acggtgtttt
     8821 atttatcata cttttccaat agggttattc gtattgcgcc tgttgcagct gaaaccgcca
     8881 atgtgatttt tatttctgaa gcggctaaag tgcctgcagg agctcaggtt attggcatca
     8941 ttcaccaaag ctattggaca tctgatgcct cttcgcgttt aaaagggaaa aattccgatt
     9001 tgccggttat ggttttcacc atgagagaaa aaacatccga agtgccataa tcgttggttg
     9061 aaatacctgt gagcgctggg tacaggtagc tttggcacac ttatggatat cattaaatta
     9121 gaaccataac cctgtaacag cgcttgatgt gtactaagga attgcctcaa tcctcgaaac
     9181 cacagtttca ataatttcct cttcgttgtg gtttatcaaa tatctgacta tgtcgcccgt
     9241 atcctccagc aatacaccca taagaaaatc gcggtaacgg atatgctgcc gcaacctgtc
     9301 gaaaatcaat ccaatcttaa cgataccttc ctggttttgc tgcatggcgt tttcaagacg
     9361 aatgcgcaat tccttttcaa gagatgaagt atcggaggca ttatattctt tcaattttgc
     9421 ttcgcggagt ttggcaagag aatgccattg ttccatctgt tgcagaaatg ccctgaacag
     9481 tgatgcatta tgcttcatga gtagtgtgtg ctcctgaaga acttctgccg acaattcatc
     9541 tgctataatg cgcaaatcgt ttgcggggag gttgtaaacc gccatttcgc ccggcgacca
     9601 tacggtattg aacacaattt gagcgttgtt actatttgcg tatctgagca atgtgggcat
     9661 gtcgtttatg tttaatcttg ttgcggtggc tgaaatcacc agattactga tgtttttgcg
     9721 cacaggcatc aacgaaataa attcgtcgat gttatgcaaa acctcgctaa ggcgtgcatt
     9781 ctttctgatc atttcaaagc gttcagcatt cagagagtcg agcgaaactc ccagggtaaa
     9841 ctgccccgat gcaagccatt tccggatgcg gtcgtttatg attgttccgt ttgtttgaac
     9901 atagatattg gcttttttat tttcacgcaa tattgcgtcc cacatttcga agtacaatgg
     9961 tatgaggaat ggttcaccac caccaaaacg caacaaccgg gcttttggtg gtggtgaacc
    10021 attcctcata ccattgtact tcgaaatgtg gaatggttca ccac
//
LOCUS       DAOOQP010000109         9941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8429_length_9941_cov_5.979567, whole genome shotgun sequence.
ACCESSION   DAOOQP010000109 DAOOQP010000000
VERSION     DAOOQP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8429_length_9941_cov_5.979567"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..565)
                     /gene="trxA"
                     /locus_tag="PLK75_10240"
     CDS             complement(167..565)
                     /gene="trxA"
                     /locus_tag="PLK75_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOY39666.1"
                     /translation="MKHIIKIIGITLAVIFSGLGESFAQNNSSGIKVLNESNFTKNTK
                     KGVVLVDFYADWCGPCKMMHPVLEELAVETSGKIKFFKVNIDNANELAAKYNIRSIPC
                     LIVFKNGREAERFVGFQQKETLLLNLGKFL"
     gene            complement(562..1479)
                     /gene="miaA"
                     /locus_tag="PLK75_10245"
     CDS             complement(562..1479)
                     /gene="miaA"
                     /locus_tag="PLK75_10245"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOY39667.1"
                     /translation="MIQDCSLIIIAGPTSVGKTEVCINLAKHFNTEIVNCDSRQMFRE
                     MQIGSGCPSPDQLKIVKHHFIGNLSVHDYYNAGRYETDVMELIESLFHRHSVLILSAG
                     SGMYLDAIMQGIDFMPEYNQEVRDELNQTLKTYGLEYLLNELEKADPVYYREVDKNNI
                     QRVLRGLEVKKLTGIPYSQYRVKQHKVRPFNMVKILLQRNRDELYQRIDKRVDSMLAA
                     GLEQEARNLYGFKQLVPLKTIGYREFFMYFDGIIDRNEAIRLIKRNTRHLARRQLTWF
                     RSKKDFTPIEADNLHEILRYIINSTSNKI"
     gene            complement(1476..2003)
                     /locus_tag="PLK75_10250"
     CDS             complement(1476..2003)
                     /locus_tag="PLK75_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39668.1"
                     /translation="MILKFRIISPEKENFARDIEIFDDSSFLELHEAIQLSCDYDPSI
                     MTSFYVSNERWEKKEEIVMQIMDEEEQKESLLMDSTPLNRFFNHKGQRLIYIYDFFSV
                     RLFLLEVVNIREFEKSDESLEFPICTLCSGIPPQQIKVEEISEMRYEDEITDDFDDLD
                     NIDPSEFDDIENYDL"
     gene            complement(2006..3034)
                     /locus_tag="PLK75_10255"
     CDS             complement(2006..3034)
                     /locus_tag="PLK75_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39669.1"
                     /translation="MNFEYRKRVLTYSGELFSAILNDEYQHRFDGFEKVFFANNWFTP
                     ENVKYALQHWSTLLTGENTESWLAEYPGIDNVSGGKNIALVFAGNIPLVGFHDLLCCI
                     ACGMNIEIKMSSKDKLLPKWYIDQIIGEFPFLRSKITFTDNYFQDFDAVIATGSNNTG
                     RYFDYYFGKYPHIIRRNRNSTAILTGNEDEEDLAKLADDIFLFFGLGCRSVSHIFVPE
                     GYCFDRLLKVCAESYPVVSYHHKYFNNYEYQKAILQFNRVSYFDSGNLLLTENDKTAS
                     PVSMLHYTYYKNLQEVQDALSSRSLILQCIVGKNTGLENTPLGQTQQPKLNEYADGID
                     TLNFLLNL"
     gene            complement(3042..3584)
                     /locus_tag="PLK75_10260"
     CDS             complement(3042..3584)
                     /locus_tag="PLK75_10260"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HOY39670.1"
                     /translation="MTDLENNSPESPSSTEKNTENENPVSENIIQPEPADTMGNNDAC
                     PEEILIQQQQIKDAQNAMKPPQPMQVKLPGSGAVLTLGILSIATISCCGPFIGPVLAI
                     IALALAARANKLYRENPDMYKGSSYGNTKAGKICAIIGLSIGAFVLTMWIINYLTGAH
                     NPQIDKIEEMYMQLWDNMNY"
     gene            complement(3581..3787)
                     /locus_tag="PLK75_10265"
     CDS             complement(3581..3787)
                     /locus_tag="PLK75_10265"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39671.1"
                     /translation="MELLHGNLAESILLYPALFPLVAMFVFLMLHLKFEFKKGHIVLK
                     FFFIINVAIIIISYFCKIFNFDLL"
     gene            3919..4566
                     /gene="aat"
                     /locus_tag="PLK75_10270"
     CDS             3919..4566
                     /gene="aat"
                     /locus_tag="PLK75_10270"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOY39672.1"
                     /translation="MAVYLLNEEIVFPDPRLADSDGLLAVGGDLSAERLLLAYSNGIF
                     PWYNPGEEIMWWAPDPRFVLFTEDFKTTKSYRSLIRNHNFAISFNQAFPKVIKACKTR
                     KRQGQPGTWITEAMRKAYITLHNKGFAVSVEVWDNDLLVAGLYGVITRHVFCGESMFH
                     TVNNAGKVAVYHLVELCKIGKIKLIDVQMHSVFFEQIGAAYIPLSEYLHILQDEL"
     gene            4563..5900
                     /gene="tilS"
                     /locus_tag="PLK75_10275"
     CDS             4563..5900
                     /gene="tilS"
                     /locus_tag="PLK75_10275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOY39673.1"
                     /translation="MRKHKSNIAERISDFVTGHEIGYNARLLCAVSGGVDSVVMLEVL
                     LKEGFKCAVAHCNFKLRGNESDGDENFVQHLTETHKLDFHVKSFATSDYALEKGVSIQ
                     MAARELRYQWFNELMNNFGYDYLALAHHADDCVETVLINLARGTGISGIAGIPEISGK
                     MIRPMIQLRRQEILAYAKKNKIKFRIDSSNNEVKYHRNLIRHRIVPLFEQINPSFTET
                     MLQNIEHFKAGNLLFAAASNELLHRCVRKNEEETIIEIDKIPEEAVRNGTFFSLLREL
                     HISANASDEVIKILSGQSGKMVFDGSKQILRDRNTLIIREKAVHEETICYIENDTRFI
                     SEPVVLQIKLIHRNHLPSIPDDSKVACIDYAKISFPLSIRKWKKGDRFIPLGMSGYKK
                     LSDFFTDNKVTRFDKENTFLLLSGTDIVWIIGMRIDERYKIAKTTEWIYLLSL"
     gene            5913..6473
                     /locus_tag="PLK75_10280"
     CDS             5913..6473
                     /locus_tag="PLK75_10280"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOY39674.1"
                     /translation="MKWLKIILVSIAFLGATAMVLLFSGCSQQKSGDNLQKHRYQSVC
                     IDAKAMPVRAEVYVPVYSDIYHIDGTRKFALTATLSIRNASTTDIFYVYSIEYYDSDG
                     RLLRNYLDSAIIVKPMHSVEFVVENKESDGGAGANFIVKWVAEKKVTNPVIQAVMIGT
                     ESKQGISFAVDGITTNYVCNDSAAIK"
     gene            6480..7103
                     /locus_tag="PLK75_10285"
     CDS             6480..7103
                     /locus_tag="PLK75_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39675.1"
                     /translation="MARILSICLAAFIILTSCNNHKTDKSEQSDSMNIASQISHDDSR
                     VFQIEHGYIKYESSKGQIREIWFDDFGRLQYEESYRIQKGVKNGSCSYVINGYKYDMH
                     LGSSEIVRSPYKAEPATEYDKLSDEIKQQYEITKIGDESVNGKYCSVISFKKPIPSTV
                     WLYKGIPMKSISSNGVEELTITAILFTEDEINPQMFVLPQGVKIVEY"
     gene            7253..7561
                     /locus_tag="PLK75_10290"
     CDS             7253..7561
                     /locus_tag="PLK75_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOY39676.1"
                     /translation="MNIYVAGIPFKASENELKGLFEEYGEVVSAKIIIDKFTQKSRGF
                     GFVEMSNDEEAQQAISSLNGSEFMGKNLIVNEAKPRTENSFNNNRNSGGYGKNPNKRY
                     "
     gene            complement(7666..8754)
                     /locus_tag="PLK75_10295"
     CDS             complement(7666..8754)
                     /locus_tag="PLK75_10295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39677.1"
                     /translation="MRIVVSVSNDIVTDQRLHRICSTLVDNKYDIILLGRKKRNSPQL
                     GEYSFATKRFRLLFEKGPMFYACLNVRIFLFLLFAKADIFLSNDLDTLPANYLASKIR
                     RKKLVYDSHELFTEVPELQGRPRIKAFWERIEKRILPNIKYSYTVCESIAAYYNQKYG
                     IDMKLVRNLPTKKEIQIPFHERGNVFIYQGVLNKGRGLEMLIESMVHLKEYQLIIAGS
                     GDIEDKLKHIAAEHKADNVVFAGRIPPDVLHALTCKAILGFSLEEDMGLNYRYALPNK
                     IFDYIQAGVPVFVSNLPEMRAIVEAYEVGRFFQKKAAMQLAQEIMDITGNKTLLLEYH
                     LNCQKAACVLNWETESVRLMELFETINI"
     gene            8828..9844
                     /gene="murB"
                     /locus_tag="PLK75_10300"
     CDS             8828..9844
                     /gene="murB"
                     /locus_tag="PLK75_10300"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOY39678.1"
                     /translation="MPAQVIKNCSLKPYNTFGIEATAEQLMSFNATEELINFIKNTEL
                     PDKIFILGGGSNVLFTADYDGLIIHPLNKGIKILEETDACAVVEVEAGEVWDDFVLWA
                     VEHKLYGVENLSGIPGSVGAVPVQNIGAYGDEVRNVIKLVKGVEISTGNAVSYLNFEC
                     RFGYRDSIFKNELKNKIIITSVVFSLSKIKNLNLKYADLANYFGSNTDSELQEVRDAV
                     CKIRDSKLPDYKLTGNAGSFFKNPVVLKSELEGLLNKFPDIRYFDYTSNHVKLAAGWL
                     IDKCGLKGFRQGRAGVHEKQALVLINIGGAEGREILRLADLVRQKVMDKFGVELEYEV
                     NILP"
BASE COUNT         3040 a         1839 c         2010 g         3052 t
ORIGIN      
        1 tgtgaaatcc atgtcccaaa tttaaaactt tccactaaat ttgcagtaga accaaagttt
       61 tgtttaccat catgctattt catttaatag atattctcaa aggtagagaa tcctattaaa
      121 aacaaaatgt gttttcgcgc aaaaatgatc cgcctttgaa acggtgttat aagaatttcc
      181 ctagattcaa cagtagggtc tctttctgct gaaaaccaac gaatcgttca gcttcgcgcc
      241 cgtttttaaa gacaattaag catggaatgc ttcggatatt gtatttggca gctagttcat
      301 tagcattatc gatattcact ttaaaaaatt tgattttacc gcttgtttca acagccagtt
      361 cttctagaac aggatgcatc attttacatg gtccgcacca gtcggcataa aagtccacaa
      421 gaacaactcc cttttttgtg tttttcgtga agttactttc gttgagtact ttaatacctg
      481 agctgttgtt ttgcgcaaaa ctttctccca aacctgaaaa gatcacagct agtgtaattc
      541 caataatttt aattatatgc ttcatatttt atttgatgtt gaattaatta tgtaacgtag
      601 aatctcatgc aaattgtctg cctcaattgg tgtaaaatct ttttttgacc tgaaccaggt
      661 tagctgtcgc cttgcaagat ggcgtgtgtt gcgtttgata agtctgattg cttcatttct
      721 atcaataatt ccgtcgaagt acataaagaa ttcgcggtaa cctatcgttt ttagaggcac
      781 aagctgcttg aatccgtaca aattacgagc ttcctgctcc agtccggctg caagcataga
      841 gtccacacgc ttgtcgattc tctggtacaa ttcatctctg ttgcgttgaa gaaggatttt
      901 aaccatgttg aagggacgca ctttatgctg ttttacacga tattgcgaat aaggaatccc
      961 tgtaagtttt ttcacttcaa ggcctcttaa tacacgttga atgttgtttt tatcgacttc
     1021 ccgataatac actgggtcgg ctttttcgag ttcgttaagc aggtactcca acccgtaggt
     1081 tttaagggtt tgatttagtt catcacgcac ttcctgattg tattccggca taaagtcgat
     1141 tccctgcatg atggcatcga gatacattcc tgagccagct gataaaatca acacactgtg
     1201 ccgatgaaac aaggactcaa ttagctccat aacatcggtt tcgtacctgc cggcattgta
     1261 gtaatcgtga acagacagat tcccaatgaa atggtgtttt acaattttta attggtccgg
     1321 ggaggggcag cctgacccga tctgcatttc acgaaacatc tgcctcgaat cgcaattaac
     1381 aatttcggta ttgaaatgct tcgccagatt tatgcaaact tcggttttac caacgctggt
     1441 tggaccggcg attataatca ggctgcaatc ctgaatcata aatcatagtt ttcgatgtcg
     1501 tcgaattcac tggggtcaat gttgtccaaa tcgtcaaaat catcagtgat ttcgtcttcg
     1561 tagcgcattt cgctgatctc ttctactttt atttgttggg gaggaatacc actgcagaga
     1621 gtacatatcg gaaattccag tgattcatcg cttttttcga attccctaat gttaactact
     1681 tcgagaagga agaggcgtac cgagaaaaaa tcgtagatat aaattaaccg ttgccctttg
     1741 tgattgaaaa agcgattcaa aggagtgcta tccattagca atgattcttt ttgctcttct
     1801 tcatccatta tttgcattac aatttcttct tttttctccc agcgctcatt cgaaacgtaa
     1861 aacgaagtca tgatggaagg atcgtagtcg cacgacaact gaattgcttc atgcaactct
     1921 aaaaaactgc tgtcgtcgaa aatttcgata tctcttgcaa aattttcttt ttccggcgat
     1981 attattctga attttaaaat cataattaca aatttaataa aaaattaagc gtatcaatac
     2041 catcggcata ttcatttaat ttcggctgct gcgtttgccc taaaggcgtg ttttccagcc
     2101 cggtgttttt gccaacaata cattgcaaaa ttaatgaacg tgagcttaaa gcgtcctgca
     2161 cttcttgcaa atttttgtaa tacgtgtaat gaagcatgga tacaggtgat gcggttttat
     2221 cgttttcggt aagcagcaga ttgccggaat cgaaatagct aacacgattg aattgaagta
     2281 tcgctttttg atattcataa ttattaaaat atttatgatg gtacgataca acagggtagc
     2341 tttctgcaca aaccttcagc aaccggtcga agcaatatcc ctcgggtacg aaaatatgac
     2401 ttacactccg gcaacccagg ccaaaaaaaa gaaatatatc atcagcgagt ttggccagat
     2461 cttcctcgtc ttcatttccg gtaagaatgg cagtcgaatt tcggtttcgg cgtattatat
     2521 gcggatattt tccaaaataa tagtcgaagt atcggcctgt gttattgctt ccggtggcaa
     2581 taacagcatc aaaatcttgg aaataattgt cggtaaatgt aattttactg cgcaggaaag
     2641 ggaattctcc aatgatttga tcgatgtacc atttgggcag taacttgtct ttagagctca
     2701 ttttaatctc aatattcatc ccgcaagcaa tgcagcacag caaatcgtga aatccaacaa
     2761 ggggtatgtt acctgcaaaa acaagcgcaa tatttttacc gcctgataca ttatctattc
     2821 cagggtattc agccagccaa ctttcggtgt tttctcctgt gagaagggtg ctccagtgct
     2881 gaagcgcata tttaacattt tcaggggtaa accagttgtt cgcgaagaat accttttcga
     2941 atccatcaaa acgatgttga tattcgtcat taagtattgc agaaaaaagc tcaccgctat
     3001 aggttaatac tcttttccga tattcgaaat tcataatgtt gtcaataatt catattgtcc
     3061 catagctgca tatacatttc ttcaatttta tcgatctgtg ggttatgtgc gccggttaag
     3121 taattaatta tccacatggt aagcacaaag gcaccaatcg acaagccaat aattgcacag
     3181 atttttccgg cttttgtatt accatacgaa gagcctttgt acatgtcggg attctcacga
     3241 tacaacttgt ttgctctggc agcaagtgcc aatgctataa tggcaagtac aggacctatg
     3301 aagggaccgc agcaacttat tgttgcaatc gatagaatcc caagggtaag aactgcaccg
     3361 gatccgggaa gtttaacctg cattggttga ggcggtttca tcgcattttg cgcatccttt
     3421 atctgttgtt gctgtatgag tatttcttca gggcatgcat cattgttacc catagtatct
     3481 gcaggctccg gctgaattat attttcgctt accgggtttt cattttccgt attcttctcg
     3541 gtagaacttg gcgattcggg agaattgttt tctaaatcgg tcataataaa tcgaaattaa
     3601 atattttaca aaaataactg attataatta tcgcaacatt aattatgaaa aagaacttca
     3661 atactatatg tccttttttg aactcaaatt ttaggtgcag cattaaaaac acaaacattg
     3721 caacgagcgg aaacaaagca ggatatagca agatgctttc ggcaaggttg ccgtggagta
     3781 attccattat tgagcgttgc atcccgcatc cgggacaatc gtatccggta atagccttaa
     3841 tagggcaagg aatcagatga tatgccagaa tacctgtcat ttgatttcaa aattatgtaa
     3901 aattatatag ttttgactat ggctgtttat ttattaaatg aagaaatagt ttttccagac
     3961 cccaggctgg ctgatagtga cggattgctt gcagttggag gcgatttgag cgctgaaagg
     4021 ctgttacttg catattcaaa tggtattttc ccatggtaca atcccggcga agaaattatg
     4081 tggtgggctc ctgatccgcg ctttgtactc tttacggaag actttaaaac cacaaaatcg
     4141 tatcgaagcc taattagaaa tcacaatttt gccatttcct tcaaccaagc gttccccaaa
     4201 gttattaagg catgtaaaac aagaaagcgg caaggtcaac caggtacctg gattacagaa
     4261 gcaatgagaa aagcatatat tacattgcat aataaaggat ttgctgtttc ggtagaagtg
     4321 tgggataacg acttacttgt tgccggtttg tacggagtga taacaagaca tgttttttgc
     4381 ggtgaatcga tgttccatac tgtgaacaat gcagggaaag ttgctgtata tcatttggtt
     4441 gagctttgta aaataggaaa aatcaagctc attgatgtgc aaatgcattc cgtatttttt
     4501 gaacaaatcg gggctgctta tataccattg tcggaatatc ttcatatctt gcaagatgaa
     4561 ttatgaggaa acataaatca aatattgcgg agaggatttc ggattttgtt accgggcatg
     4621 aaattggtta caatgctcgt ttactttgtg ctgtaagcgg aggtgtcgat tctgtcgtta
     4681 tgctggaagt actattaaaa gaaggtttta aatgtgcggt tgctcactgt aatttcaaac
     4741 tccgtggcaa tgaatcggat ggagatgaga actttgtgca acacctcaca gaaacgcata
     4801 aacttgattt tcatgttaaa tcatttgcta cttccgatta cgctctggag aaaggggtaa
     4861 gcatacaaat ggctgcccgc gagctgcgtt accaatggtt caacgagtta atgaataatt
     4921 ttggttacga ttatctggca cttgcccatc atgctgacga ttgtgtggaa accgtgctga
     4981 ttaacctggc ccgtggcacc ggcatcagtg gtattgcagg aattcccgaa ataagtggaa
     5041 aaatgatccg gccaatgatt cagctcagac ggcaggaaat actggcctat gcaaagaaaa
     5101 ataaaataaa attccgaatc gactcttcga acaacgaagt taaatatcac cgcaatttaa
     5161 tacggcatcg cattgtacct ttattcgaac aaatcaaccc ttcattcacc gaaacaatgc
     5221 tgcagaatat tgaacatttt aaagcgggta atttattatt tgcagctgct tcgaatgagc
     5281 tgctccatcg ttgcgtacga aaaaacgaag aggaaaccat catagaaatc gataaaattc
     5341 cggaagaagc agtcagaaat ggtacatttt tctcactgct aagggagctt cacatttcgg
     5401 caaacgcatc ggacgaagta attaaaatat tgtcgggcca atccgggaaa atggtatttg
     5461 atggtagcaa gcaaattctt cgtgacagaa atactcttat tattcgtgaa aaagcagtac
     5521 acgaagaaac catatgctat atcgaaaacg atacccgatt tatatctgaa cccgttgtgc
     5581 ttcaaataaa gcttattcac agaaatcact tgccaagtat tcctgacgat tcaaaagtag
     5641 cctgtatcga ttacgccaaa atatcttttc cgctctctat acgcaaatgg aaaaaaggag
     5701 accggtttat ccctttggga atgagcggtt ataaaaagct tagcgacttt tttaccgata
     5761 ataaggtaac aaggttcgat aaggaaaaca catttttgct gttatcggga actgacattg
     5821 tatggataat cggaatgcgg attgatgaaa gatataagat tgcaaaaacg accgaatgga
     5881 tttatttact tagtttataa aaattgttag aaatgaaatg gttaaaaatc atacttgtgt
     5941 cgattgcatt tttgggtgca acagcaatgg tattattatt ttcaggttgt agtcagcaaa
     6001 aatcaggaga taatttgcag aagcaccggt accagagtgt ttgtattgat gcgaaagcaa
     6061 tgccggtgcg tgcagaagta tatgtacctg tgtactccga catttatcat atcgatggaa
     6121 cgcggaaatt tgcccttaca gcaacactaa gtataagaaa cgcaagcact accgatattt
     6181 tttatgtgta ttcaatagaa tattatgatt cagatgggag actgctccga aattatcttg
     6241 attctgctat tattgttaaa ccgatgcatt cagttgaatt tgtagttgag aataaagaat
     6301 cggatggagg agcgggagct aattttattg tgaaatgggt ggctgagaaa aaagtgacaa
     6361 atcctgtaat acaggctgtt atgataggta ctgaaagcaa acagggtatt tcttttgcag
     6421 tggatggaat aactacaaac tatgtgtgca acgacagtgc agcaataaaa tagttaggaa
     6481 tggcgagaat tttaagtata tgtttggcag cttttatcat tctgacgtcg tgcaataatc
     6541 ataaaaccga taaatctgaa caatcggatt caatgaacat tgcttctcaa atttcacacg
     6601 atgacagcag agtttttcag attgaacatg gatatattaa gtacgaatca tcaaaagggc
     6661 aaatacgaga aatttggttt gacgattttg gcaggcttca gtatgaggaa tcgtaccgga
     6721 tacaaaaagg agtaaaaaat ggaagttgtt cttatgtgat aaatggatat aaatacgata
     6781 tgcacctggg ttcttccgaa atagtgcgga gtccttataa agctgagcct gctactgaat
     6841 acgataagct ttctgatgaa ataaaacaac aatatgagat cacaaaaatt ggagatgaaa
     6901 gcgttaatgg taaatattgt tccgtaatca gctttaaaaa accaataccc agcacggtgt
     6961 ggctttacaa gggtattccg atgaaatcga taagtagtaa tggagttgaa gagcttacta
     7021 ttactgctat tttgttcacg gaagatgaaa taaacccgca aatgttcgtc ttgccacagg
     7081 gcgtgaaaat tgttgaatac tgagcttaga aattggtatt gcataaatct ctgaccattg
     7141 cttaattatt tcctttattt ctgttgtgtt tgtaaattat atttgtatat ttgccgagtt
     7201 ttacatgtgt aaagcgagta attataatta ttaaacaaat ttagaagaag aaatgaacat
     7261 ttatgtagca ggaattcctt ttaaagcttc tgaaaacgaa cttaaaggat tattcgaaga
     7321 gtatggcgaa gttgtatctg caaagattat catcgacaaa tttactcaaa aaagcagagg
     7381 ttttggtttt gttgaaatga gcaacgatga agaagcccaa caggctatca gctcgttgaa
     7441 cgggtcagaa tttatgggta aaaacctaat tgtgaacgaa gctaaaccca gaaccgaaaa
     7501 ctcattcaat aacaaccgga acagcggcgg ttacggaaaa aaccctaaca aacgttatta
     7561 atattgttat tgaaacccct cggaaagcct cagtgatgag gctttttttt ttgaagtgct
     7621 cagaaaaaat caataatttc gcttgatacc aaaacacaat caaagtcaaa tattaatggt
     7681 ttcaaatagt tccatcaacc taacgctctc agtttcccaa ttcagcacac acgcagcttt
     7741 ttggcagttt aagtgatatt ctagtaacaa tgttttattg ccggttatgt ccataatctc
     7801 ctgtgccaac tgcatcgctg cttttttctg aaaaaacctg ccaacctcgt atgcttccac
     7861 aattgctcgc atttcaggca ggttgcttac aaacaccggc acacctgcct gtatataatc
     7921 gaaaatcttg ttgggcaatg catagcggta attcagcccc atatcttctt cgagcgaaaa
     7981 tccgagaatt gccttacagg taagtgcatg aagcacatca ggcggaattc ttcccgcaaa
     8041 cacaacatta tcggctttgt gttcagcagc aatatgttta agcttatctt caatatctcc
     8101 gctgccggca atgattagct gatattcttt taaatgaacc atagattcaa tcagcatctc
     8161 gagtccacgg cccttattca aaactccctg ataaataaat acattccctc tttcatggaa
     8221 aggaatctga atttcttttt ttgtaggtag gtttctgacc agcttcatat caattccata
     8281 tttttgattg tagtaagctg caatactttc gcacactgta tagctgtatt taatgttggg
     8341 aagtattcgt ttttcgatgc gttcccagaa tgcttttatg cgtggccggc cttgcagttc
     8401 tggtacttcc gtaaacaatt catggctgtc gtataccagt tttttgcgtc gaattttcga
     8461 ggcaagataa ttggcaggca atgtatcgag gtcattgctc aaaaaaatat cggcttttgc
     8521 aaaaagtaaa aacagaaata ttcggacatt cagacaagca taaaacatcg gtcctttttc
     8581 gaaaagcaac cgaaaccgct ttgttgcgaa tgaatattcg cccaactgcg gagaatttcg
     8641 ttttttacgt ccaagcaaaa taatatcata tttattgtca accagtgtac tgcaaatacg
     8701 atgcagccgc tgatcggtta caatatcgtt gctaactgaa actactattc tcacggatgc
     8761 tgtttttcga ttttaaaatt atacttttga aacgaataaa acttaatttt taatataaaa
     8821 ttcgattatg cctgctcagg ttattaaaaa ttgttcgctc aaaccgtata ataccttcgg
     8881 tattgaagct acagcagaac aattgatgag ctttaatgct actgaagagt taataaactt
     8941 tatcaaaaac acggagttac ctgataaaat attcatactt ggtggtggaa gcaatgtgct
     9001 ttttactgct gattatgatg gtttgattat tcatccccta aataaaggaa ttaaaatatt
     9061 ggaagaaacc gacgcttgtg ctgttgtgga agtcgaagca ggggaagtgt gggacgattt
     9121 cgttctttgg gctgtggagc ataaattgta tggagtggaa aacctttcgg gtattccggg
     9181 ttcggtgggt gcggttccgg tgcagaatat cggggcctat ggcgacgagg ttcgtaatgt
     9241 tataaagctg gtgaaagggg tggaaatatc aaccggaaac gcggtttcat accttaactt
     9301 tgaatgtcgc tttggatatc gtgacagtat ttttaagaac gaattgaaaa ataagattat
     9361 tattacttcg gtggttttca gcctttcgaa aattaagaac ctgaatttaa aatacgctga
     9421 cctggcaaat tattttggaa gcaacactga ctctgaactt caggaagtga gagatgctgt
     9481 ttgcaaaatc cgtgattcca aactgcccga ttataagctt accggaaatg caggtagctt
     9541 tttcaaaaac ccggtggtat taaagtcaga attagaaggc cttctgaaca agtttcctga
     9601 tatccgatat ttcgattaca cctctaatca tgtaaaactg gctgcaggtt ggctcattga
     9661 taaatgtggt ttaaagggtt ttcggcaggg cagggcaggg gttcatgaga agcaggcgtt
     9721 agtactgatt aatattggag gagccgaagg gagagaaatc cttcgtttag ctgatttggt
     9781 aaggcaaaaa gtaatggata aatttggtgt agaactcgag tatgaggtaa atattttacc
     9841 ataaaaaaag acgctgctcg cgtctttctg taattcaaat tttaatctcc tgacttcgac
     9901 aatgcgccac cctaaaaatt ctcccaaaag ttttcttcaa a
//
LOCUS       DAOOQP010000110         9635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8788_length_9635_cov_4.607098, whole genome shotgun sequence.
ACCESSION   DAOOQP010000110 DAOOQP010000000
VERSION     DAOOQP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_9635_cov_4.607098"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1202
                     /locus_tag="PLK75_10305"
     CDS             120..1202
                     /locus_tag="PLK75_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY39679.1"
                     /translation="METLAWIGFTQSLFGALLLLTKPNTRLPDRLLTVWMVICAITFI
                     EIAIAKAESFTITPINAMITNVLLYFYSILLTKENFKFRSKHFYHALLPVVFILVIVW
                     TGAKLRIDQYFYEDVFLPYRLVLTVSLIVSFFLYWFLSIRAIHRYRMQLKNEYSSIEA
                     GIKLNWLLFVLIFYILYNLVLITAGFVEVFSAIETNYILFILIFNLFLVFAFTFYGLR
                     QNALLPVAQNQLEQEAYSNSRLDDDEKTLIKQKLLQHFEKNEPYIDGSLTITSLANQL
                     KVQRYKLTEVLNTELKMNFYRFVNEYRVEKVKKMLTDPAYARLSIEAVGYDCGFNSKS
                     TFFTVFKNLVGMTPAKYQEKHKTRKL"
     gene            1385..2689
                     /locus_tag="PLK75_10310"
     CDS             1385..2689
                     /locus_tag="PLK75_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39680.1"
                     /translation="MKVILICAVAFFAVISEIVYAQEVPDSNEINRPASNYSGSIRGF
                     AFAGSERFDYSCAFAEAGFRVDANLKKTLTFRSDLRFRYGYQFNEEKTILDIREAYAG
                     FVSKPFSVSLGKKVLDWSRVDAYSVSNRLTPTDYFYFSPDADDQKLSQWMIHTKVMFG
                     NRISWQISAIPFFAPSVYRFDLFDMGENAVFEQAVMPERTFSNGSIASRLNFELNAIG
                     FAFSYFEGYATEYGFTLSGVNFFNDPIVATLRPVYHKMRSASIDFEVPAGSWIFRGEF
                     AFNQIKDTGNMMYLPRNGLHYVFAVEKSFFNITSMAEYIGHFTPDFESLATPTNVIED
                     MLYQLFNFNRQIFYQQEKYNHAVALIFTRDFYYNAIQANLALYYNITSEEFTVRPQIS
                     YSVNDNLKVTVGGMYMRGPENSIFSYSSEIMNGAFVQLKASF"
     gene            2690..4942
                     /locus_tag="PLK75_10315"
     CDS             2690..4942
                     /locus_tag="PLK75_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY39681.1"
                     /translation="MHNTSNFWFFRYRFAILGAAIMLIILGIIPFFWVQVDSDLKNYF
                     PNEMDALKNTKRIEESFGTTEPVLLLLCSDNVLKKTTLKRFSTICSEIEEQEWADKVI
                     SVFNSKNIHSEGGMMLVDEAIPFYPENKAELDQLTSLIDNNPLISGFLVSENHRYMLA
                     MVSKKPNSDDKQINESISNILKANPGTDDILVAGEPQLRAVINKDIKKDLVILLPLGI
                     LLMLLFLWASFKNIRAVFIPIFIVCISIILSVGLMPVLGWKLSIISILIPVMMLAIAN
                     NYGIHFVSAYREELSERQHDDKKVLVQSVFRRLKYPVLLTGLTTIAGILTMVTHVMIP
                     ARQTGIIAGWGITAALVFSMFLVPVLLVLADKKHFAPQSKKERPTNTPLVLDKMSRWV
                     TKSPKLIIVGGAIVMIASGYGISRLAVDPNSENVLPAKHEFLQANTLINENFGGSKNF
                     SVMIEGDIKDPALLRKIDSIGTTLKSLPEVGNVISIATVIKTMSTALYDTGDSLCGKI
                     PDTREAVAQILELYAMGGDPSDFEQIVDFNYEKTVMSVQFKANSNADYKNVFSTLDSL
                     ISDIDTPVIYGGYALITYEMIDAIIRGQTYSLALSVVIVFVLLLWIFRSWRYALLGCV
                     PLSATILIMFGMMGVFGFSIDVISAMISSIVVGIGVDYTIHFLWRYRLEKQNGRNVRD
                     AVYKTLTTTGKGIIINSLSVMIGFVVLLFSSFAALKSFSFLIITALLMCLFFSLLVVP
                     ALCIISSKEK"
     gene            4939..5355
                     /locus_tag="PLK75_10320"
     CDS             4939..5355
                     /locus_tag="PLK75_10320"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOY39682.1"
                     /translation="MIKTIITIVFLLNSCIAFCQMVLTIEISKLEKSSGTIFIGLFDN
                     AQSFPKEGNAYRDIRLKAVAPTTTTSVQLPCGTYAIAVFHDCNNNGKCDTNFLGIPTE
                     GYGFSNNVKPKLSAPAFKHTCFELNSDRTLKINLIY"
     gene            5398..6180
                     /locus_tag="PLK75_10325"
     CDS             5398..6180
                     /locus_tag="PLK75_10325"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOY39683.1"
                     /translation="MKTLPFIVTASLLLFVQTVFCQTAREISDKASSAINLTTFEMTQ
                     TLKIIDAKGNQRVRQTTSKSLKSTDSFKTLITFNSPADVAGTSMLVYDYDKKEDDMWI
                     YMPALRKSRRIVSSEKSQNFMGSEFTNADMSKPNPDEYTYKLLPSTKYDGVDCYVVEI
                     TPLNKDVSSKMGFSKKNCYIEKSNYLTRKVEYFDATGKKVKEMVYKNYEMIDTVNKKY
                     FAKYMEVVNLSNGRKSIITVDKINTKAQIKESDVSPAVLGAL"
     gene            complement(6232..8046)
                     /locus_tag="PLK75_10330"
     CDS             complement(6232..8046)
                     /locus_tag="PLK75_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOY39684.1"
                     /translation="MWRVRINKIREFLNIRIYASKKAVLLSLQILSVIVTIAVIASVV
                     YYYGFPQNDLTSKICLLVIKISLVFYIVRYLLRLIYEFSPLKYLKNTWIEAAISFIMI
                     VAGLLFIIFRIPFFHTTAFHDYFPYSGSFVILLLQAFFIIVFIVDMSKAVQKIKILRI
                     GPAGLLSLSFMVLIFAGAGLLMLPEMTYNGEISFINALFTSTSASCVTGLTVVDTAGF
                     FTTKGKIIIMLLIQLGGLNIITFAAYFATFFRTAGIKYQSLMQDMFSAESIADSKHLL
                     RSIVLFSLIMEIIGSVAIFLQWSDSVQFNSLGQKIFYSVFHTVSAFNNAGFSLYTNNL
                     YEGVVQHAYGIHMTIALLIVFGGLGFTVMSDIFSPRMIYRRIKKPWIRLRVHTKLALF
                     TSLMLIVFGAVMFYLLEQHNAVDKSSIGGTVVSSVFQSVTCRTAGFNTVDFTKLGQPI
                     LIVFMLLMFIGASPVSTGGGIKTTTFAMLIKSAMATIRGKNNITLYKKNIPFEVIDRA
                     YSVLFFSISVIIISVFFLTITEPNTRFIQLMFEEISAFGTVGLSTGITSGLSLGGKII
                     ILTSMFIGRIGPLTLVLALVNRSGSERYRYPSASIIIG"
     gene            complement(8058..8750)
                     /locus_tag="PLK75_10335"
     CDS             complement(8058..8750)
                     /locus_tag="PLK75_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOY39685.1"
                     /translation="MKSEKFAVIGIGQFGHVIAKELSNKGAEVLAIDNDPEVIERVSE
                     DVALAVACEASDKKALAAHNITDFDGVVVALGHNFEELLTCCITLLDMKVKRIIARSS
                     GPNQESILRKIGIKEILSPEAEVGILVAERLMNPSIISYLQLPDEYRIAEIKAPSKFI
                     GRSIKDISPRSKYNLNIITIKRLFQNDNNQENPVQHITGVPDSSYQIEKSDTLIIFGK
                     NKDIERMIEIND"
     gene            9017..9406
                     /locus_tag="PLK75_10340"
     CDS             9017..9406
                     /locus_tag="PLK75_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOY39686.1"
                     /translation="MPEIQKELTRAELQIMQILWSIEKGFVNDILVALPEPRPAYNTV
                     STIVRILEKKGFVSYVAYGKTHEYFPLISKEQYTREYVGGVVKNYFQNSFRQMLSFFA
                     NENKLSVNEIEEIRKLMEEELERKKNK"
     gene            9417..9608
                     /locus_tag="PLK75_10345"
     CDS             9417..9608
                     /locus_tag="PLK75_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39687.1"
                     /translation="MEWFITYSIKASLLTTIFFGVYHLIMKGDTHFRLHRYYLVSTAL
                     LSYLLPLIPLSFSGGASDL"
BASE COUNT         2972 a         1834 c         1917 g         2912 t
ORIGIN      
        1 agcgtgttgt tctgaagaaa taacgaaaca aatgcaatta aagagggtgt cattaaattg
       61 atgccctctt ttttttatac tattttctct ttagctgcgt tttatcctat atttgtagta
      121 tggaaacact ggcatggata ggttttacac aatcgttgtt cggagcatta ttacttctca
      181 ccaagccgaa tacacgctta cccgatagat tacttactgt ttggatggtg atttgtgcaa
      241 tcacttttat cgaaattgca atagccaaag cagaaagttt tacgattacc ccaattaatg
      301 caatgatcac caatgtattg ctttattttt attccatttt gctgacaaaa gagaatttca
      361 aatttcgttc aaagcacttt taccatgcac tgttaccggt tgtatttatt ctggttattg
      421 tatggacggg tgcaaaactt cggatcgacc aatatttcta tgaagatgtg tttctaccat
      481 accgactggt gctaacggtt tcgctgattg tgtctttttt tctatactgg tttctgagca
      541 tcagggctat acatcgttac aggatgcaat tgaaaaatga gtattcgtct attgaggcag
      601 gtataaagct gaactggctt ttgtttgttc ttatttttta tatactatat aatctagtac
      661 tgattactgc gggttttgtc gaagtgtttt ctgcaatcga aaccaattac atattattta
      721 ttttaatctt caatttgttt ctggtttttg cttttacctt ctacgggtta cgtcaaaatg
      781 ctttgttgcc tgttgcacaa aaccagttag agcaggaggc ttacagcaat tctcgattgg
      841 atgatgacga gaaaacattg attaaacaga aactcctgca gcatttcgaa aaaaatgagc
      901 cttatattga tggctctctt accattacct ctctggcaaa tcagctgaaa gtgcagcgct
      961 ataaactcac cgaagtcctg aataccgagc taaaaatgaa cttttaccga ttcgttaacg
     1021 aataccgtgt tgagaaagtt aaaaaaatgc tcaccgatcc tgcatacgca aggttatcta
     1081 ttgaagctgt tgggtacgat tgtggcttta atagcaaatc aacttttttc acagttttta
     1141 aaaaccttgt cggaatgact ccggcaaaat atcaggaaaa gcataaaacc cggaaactct
     1201 gaaaaaaatc aagttttaac ccgctccagg tttgatatgc acaatcagaa ctcgtaaata
     1261 tcaataaaac agtatgttat gatgtgaggg ttcgattcgc ttgtccggtt taatttgtgg
     1321 ttttatttgt gaatctaaaa tcaaaaacaa tcataagtca gcgttatttg cataaaatga
     1381 cgttatgaaa gttatattga tttgcgcagt tgcctttttt gccgttattt cggaaattgt
     1441 ctacgctcag gaagttcccg attctaatga aattaatcga cccgccagta attattccgg
     1501 tagtatacga ggctttgctt ttgccggctc agaacggttc gattattcct gtgcatttgc
     1561 agaggctgga ttcagggtag atgcaaatct taaaaaaacg ctgacttttc gttccgattt
     1621 gcgttttcgg tatggatacc agtttaacga agagaaaacc atattggata ttcgcgaagc
     1681 ctatgcagga tttgtatcga agccattctc tgtttcgttg ggtaagaaag tgctcgactg
     1741 gagccgtgtt gatgcctata gcgtttcgaa caggcttact ccgaccgatt atttctattt
     1801 ctcgcccgat gccgacgacc agaagctgtc gcaatggatg atacatacca aagtaatgtt
     1861 tggaaaccgt atatcttggc aaatttctgc aatcccattt ttcgctccat cggtatatcg
     1921 tttcgattta ttcgatatgg gagaaaatgc tgttttcgag caggctgtta tgccggaacg
     1981 aacattcagt aatggcagca tagcctcaag gttgaacttt gaacttaatg caattggctt
     2041 tgcattttcg tacttcgaag gctacgcaac agagtatggt tttactttaa gcggagtgaa
     2101 ctttttcaat gaccccattg tagcaaccct gcgacctgtt taccataaaa tgaggtcagc
     2161 gtcaatcgac tttgaagtgc ctgcgggttc ctggatcttt cgcggagaat ttgcatttaa
     2221 tcaaattaag gacactggaa atatgatgta cctacccaga aatgggcttc attatgtttt
     2281 tgccgtggaa aaatcatttt tcaatattac ttcaatggcg gagtatattg gccatttcac
     2341 tccggatttt gaatctctgg ctacaccgac aaatgtaatt gaggatatgc tataccaact
     2401 gtttaatttt aaccgacaga tattttatca gcaggagaaa tacaaccatg ccgtagctct
     2461 aatttttacg agagattttt attataatgc aattcaggca aaccttgctc tgtattataa
     2521 catcacatct gaagaattta ctgttagacc ccagatttca tattctgtga acgataatct
     2581 gaaagttacg gttggaggta tgtatatgag gggacccgag aactcgattt ttagctattc
     2641 atccgaaatt atgaatggcg catttgttca attaaaagcc agtttctaaa tgcataacac
     2701 ttcaaatttc tggtttttca ggtatcgctt cgccatactt ggtgcagcca taatgcttat
     2761 tatactggga ataataccat ttttctgggt tcaagtggac tccgacctga aaaactattt
     2821 tcccaacgaa atggatgccc tcaaaaatac aaagcgtatt gaggaaagct ttggaacaac
     2881 cgaacctgtt ttacttttgc tttgttctga taatgttctt aagaaaacaa cactcaagcg
     2941 cttcagtact atttgttccg agattgaaga gcaggaatgg gctgacaaag ttatatcggt
     3001 tttcaattct aaaaatattc attcagaagg tggaatgatg ctggttgatg aagctatccc
     3061 attttatcct gaaaataagg ctgaacttga ccaattaact tcgcttattg ataataatcc
     3121 attgatttca gggtttcttg tgtcggagaa ccatcgctat atgcttgcca tggtgagtaa
     3181 aaagccaaat tccgatgata agcaaattaa cgaatcgatt agcaatattc ttaaggcaaa
     3241 ccctggaaca gatgacatac ttgttgcagg agaacctcag ttaagagcag taatcaataa
     3301 ggatataaaa aaggatctgg tgattctttt gccccttggt attttactca tgttgctttt
     3361 cttgtgggct tcatttaaaa atatcagagc agtgtttatt cccatattta ttgtttgtat
     3421 ttctattatt ctttctgtag gcctaatgcc ggtattgggg tggaaactca gcattatcag
     3481 tatactgata ccggttatga tgctggcaat tgcaaataac tatggaattc actttgtatc
     3541 cgcctatcgg gaagagttat ccgaacgaca gcatgacgat aaaaaagtat tggtgcaatc
     3601 cgtcttcaga cgattgaagt acccggtatt gctaacaggg ctaacaacaa tcgcaggtat
     3661 acttaccatg gtaacgcatg taatgatccc tgcacgacag actggcatca ttgccggatg
     3721 gggaattacg gctgcactgg tattcagtat gtttctggtt cctgttctgc tggtactcgc
     3781 cgacaaaaaa cactttgccc ctcaaagtaa aaaggaaaga cctacaaaca ctccattagt
     3841 tcttgataaa atgagccgtt gggtaacaaa aagtccaaaa ctaattatcg taggaggcgc
     3901 aattgtaatg atagcttccg gttatggaat atcgagactt gcagttgatc caaattccga
     3961 aaacgtgctt ccggcgaagc atgagttttt gcaggcaaat actctgatta atgaaaactt
     4021 cggtggctca aaaaactttt ctgtgatgat tgaaggcgac attaaagacc ctgctttatt
     4081 gagaaagatc gattcaattg gtactactct aaaatcactc cccgaagtag gaaatgttat
     4141 ttcgatagcc acggttataa aaaccatgag cacagcctta tacgatacag gcgattctct
     4201 ctgtggtaaa attcctgata caagggaagc cgttgcccag atactcgaat tgtatgcaat
     4261 gggaggcgac ccgtctgact ttgaacagat agtggatttt aattacgaaa aaactgtgat
     4321 gtctgttcag tttaaagcaa atagtaatgc tgactataaa aatgttttca gtacactcga
     4381 ttcgctcatc tcagatattg atactccggt gatatatggt ggttacgcat taattaccta
     4441 cgaaatgatt gatgccataa ttcgcggcca gacatattct ctcgcattat cggttgtaat
     4501 cgtattcgtt ttgctgcttt ggatttttcg ctcatggcgt tatgccctgt tgggatgtgt
     4561 gccactttca gctaccattc ttataatgtt cggtatgatg ggcgtgtttg gtttttcgat
     4621 agatgttatc tcagcaatga tatcttcgat agtggtgggc attggagtgg actatacaat
     4681 tcatttcctg tggcgctaca ggctagaaaa gcaaaatgga cgcaatgtgc gcgatgcagt
     4741 atacaaaaca ttgaccacaa ccggcaaagg aattataata aactcactgt cggtgatgat
     4801 aggctttgtt gtgttgcttt tttcttcgtt tgctgcactg aaaagcttct catttttaat
     4861 catcactgct ttgcttatgt gtctgttttt ttcactgttg gtagtacctg ccttatgtat
     4921 tatttcaagt aaagaaaaat gattaaaacc attattacaa tagtattcct tttgaatagc
     4981 tgcatagcat tttgtcagat ggtactcacg atcgaaatca gcaaacttga aaaatcatcg
     5041 ggcacaatat tcataggttt gttcgacaat gcccagagtt ttccgaagga aggcaatgct
     5101 tatcgcgata tcaggcttaa agctgttgca ccaactacta ctacaagcgt tcaattacct
     5161 tgtggcactt acgctattgc tgtttttcac gattgcaata ataatgggaa gtgcgatacc
     5221 aattttttgg gtattcccac cgagggctat gggttctcca acaatgtaaa acccaaatta
     5281 agcgcacctg cattcaaaca tacatgcttc gaactgaatt cggatagaac tttaaaaatc
     5341 aatcttatat actgacataa ttattaaaac aaaatgaata aattaaaaat aactgcaatg
     5401 aaaacactac catttatcgt aacagcatcg ctgcttttgt ttgtgcaaac ggtattctgc
     5461 cagactgcac gtgaaataag cgacaaagct tcttcagcaa ttaacctgac cacattcgaa
     5521 atgactcaga cactgaaaat tatcgatgct aaagggaatc agcgtgttcg gcaaactacg
     5581 agtaagagcc tcaaatcaac agactctttt aaaacactga ttacgtttaa ctcaccggca
     5641 gatgtagcag gaacatcgat gcttgtgtat gactacgaca aaaaagaaga cgacatgtgg
     5701 atttatatgc ctgcattacg aaaatcgaga cgcattgtca gttctgaaaa aagccagaat
     5761 ttcatgggct ccgagttcac caatgccgat atgagtaaac ccaacccgga tgaatacaca
     5821 tataagcttc ttccttcaac aaaatacgat ggtgttgact gttatgttgt ggaaattact
     5881 cctttgaata aagacgtaag ttctaaaatg ggtttctcaa aaaagaactg ctatatcgaa
     5941 aaatcgaatt atcttacaag aaaggttgag tattttgatg ctacagggaa aaaagttaaa
     6001 gaaatggtgt acaaaaatta cgaaatgatc gacactgtca acaagaaata ctttgcgaag
     6061 tatatggaag ttgtgaacct ctcaaacggg cgcaaatcaa ttattactgt cgacaagatt
     6121 aatacaaaag ctcagattaa agaaagcgat gtcagtcctg cggttttggg tgcattgtga
     6181 ttgctttatg taaccggttg atgcagtgtc gttgtgaaaa gctaagaagg tttagccgat
     6241 aattatactg gcacttggat atctgtaacg ttcagaacca ctgcggttaa caagagcaag
     6301 aacaagcgtg agcggcccaa ttctgccaat aaacattgag gttaaaataa tgatttttcc
     6361 acccagcgat aatccggaag taatgccagt tgataaccct acggtgccaa aagcagatat
     6421 ctcttcgaac atcagttgaa tgaaccgcgt attgggttcg gtaatagtga gaaaaaacac
     6481 cgatataata ataacactaa tcgaaaagaa cagaacagaa taagcccgat cgattacttc
     6541 gaagggaatg ttttttttgt aaagagtaat attgtttttc cccctgatgg tggccattgc
     6601 cgattttata agcatagcga aagtggtggt tttaatgccg cctccggtcg atacgggtga
     6661 ggctccaatg aacatcagca gcatgaaaac tatgagtatg ggctgcccta attttgtgaa
     6721 atcaacggtg ttgaatcccg cagtcctgca ggtaaccgat tggaaaacac ttgaaactac
     6781 ggtgccgccg atactgctct tgtcaactgc gttatgttgc tccaacaagt agaacatcac
     6841 agctccgaaa acaataagca tcagcgaagt gaataatgca agttttgtgt gaacacgaag
     6901 gcgtatccat ggttttttaa tgcgccggta aatcattctg ggtgaaaaaa tatcactcat
     6961 caccgtaaac ccgagacccc caaatacaat aagcagggca atggtcatgt gtatgccata
     7021 cgcatgttga acaacgcctt catacagatt gtttgtgtat agtgagaacc ctgcgttgtt
     7081 gaatgcagaa actgtgtgaa acaccgaata gaaaatcttc tggcccaaag aattgaattg
     7141 gacactgtct gaccattgaa ggaatattgc aacggaccca ataatctcca taatgaggct
     7201 gaacagcaca atgctgcgta aaagatgctt gctatcggct atactttcgg cactgaacat
     7261 atcttgcata agcgactgat atttaatgcc tgcagtccgg aaaaaagtgg caaaataagc
     7321 tgcaaaggtt ataatgttca aaccaccaag ttgtatcagc agcatgataa taattttacc
     7381 tttagtggta aaaaagcctg cagtatcaac cactgtaagt cctgtaacac aagaagcact
     7441 ggttgaagtg aagagcgcgt ttataaaact gatttcacca ttgtatgtca tctcggggag
     7501 catcagtaaa cctgctcctg caaatatcaa gaccatgaac gataacgaaa gcaaccccgc
     7561 aggcccaatc cggagaattt taatcttctg gacagcctta ctcatatcta ctatgaaaac
     7621 aataatgaaa aaagcctgca gaagcaatat cacaaaagag cctgaataag ggaaatagtc
     7681 gtgaaatgcg gtagtatgaa aaaatggtat tcgaaaaata atgaacaata atccggctac
     7741 aatcattatg aaacttatag ctgcttcaat ccatgtgttt ttaaggtact ttaaaggact
     7801 gaattcataa attaacctga gtaaatatcg aacgatatag aagacaaggc tgattttaat
     7861 aaccaaaagg catatttttg atgtgaggtc gttttgcgga aaaccatagt aatatacaac
     7921 actggctatt accgcaatgg taacaataac tgaaagaatt tgcagcgata gcaacacggc
     7981 ttttttgctg gcataaatgc gtatgttcag gaattccctg attttattta ttcttacccg
     8041 ccacataaca cctgttacta gtcgtttatt tcaatcattc tttcgatgtc cttattctta
     8101 ccgaaaatta tcagcgtgtc cgacttttca atctggtagc tcgaatcagg aactccagta
     8161 atatgctgca ccggattttc ctgattatta tcgttctgga acaaacgttt aatggtaatg
     8221 atattcagat tatacttact acgcggcgat atatccttga tggaacgtcc aatgaatttt
     8281 gaaggggcct taatttcagc aatcctgtat tcatcgggta gctggagata cgaaatgatg
     8341 ctggggttca tcagccgctc agcaaccagt attcctacct cggcttcggg agaaaggatt
     8401 tctttaatgc ctatttttcg cagtatagac tcctgattcg gtccggaaga acgggcaatt
     8461 atacgtttta ctttcatgtc gaggagagta atacaacagg ttaagagttc ctcaaaatta
     8521 tgtccaagag caactacaac cccatcgaaa tcggtaatgt tatgcgctgc aagagctttt
     8581 ttgtcggatg cttcgcaagc aaccgccagt gcaacgtctt ccgaaaccct ttctattacc
     8641 tcagggtcat tatcaatggc tagaacctca gcccccttat tcgacaattc tttagcaata
     8701 acatgcccga actgtccaat tccaataact gcaaattttt ctgatttcat accggagaaa
     8761 tattgcgtca aatatatagc aattaatgtt ttattggtaa aaatatttta tgggaaatgg
     8821 tacaattaag aaacgctttg cttcttgaat aaggtatgac ttagcgcgca agaaacaaat
     8881 ttttatatag atgattgtta ttgttttcag ccgttaaatc gtatatatta attttatttt
     8941 gatataaaaa ctaaaaatat agttgtataa ctaaaaaatt agttgtatat ttgtttcaga
     9001 aattaattca ttaaaaatgc ctgaaataca aaaagaactt acccgggctg aacttcagat
     9061 aatgcagata ctctggagca tagaaaaggg ttttgtcaac gacatcctcg ttgcgttgcc
     9121 agaacccagg cctgcttata ataccgtttc gacaatagtg cgtattcttg aaaaaaaagg
     9181 atttgtatcg tacgtagcgt atggaaagac ccatgaatat tttcccttga tctcgaagga
     9241 gcaatataca cgcgaatatg taggaggggt ggtaaaaaac tacttccaga attcattcag
     9301 gcagatgttg tcgttctttg ctaacgaaaa caagctgtcg gtgaacgaga tagaagaaat
     9361 acgtaaattg atggaagagg aactggaacg caaaaaaaac aaataaaaaa ccgtatatgg
     9421 aatggttcat cacatatagc attaaagcaa gtttgctaac taccattttc tttggtgtgt
     9481 atcacttgat aatgaaaggc gatacccact tcaggctgca ccggtattac cttgtaagta
     9541 ccgcattact atcctatctt ttgccactga tccccctgag cttctccggt ggggccagcg
     9601 atctttaaat tgcggatatc ctccagagat ttaaa
//
LOCUS       DAOOQP010000111         9568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8870_length_9568_cov_6.571744, whole genome shotgun sequence.
ACCESSION   DAOOQP010000111 DAOOQP010000000
VERSION     DAOOQP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8870_length_9568_cov_6.571744"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..281)
                     /locus_tag="PLK75_10350"
     CDS             complement(87..281)
                     /locus_tag="PLK75_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39688.1"
                     /translation="MKLYITLYILILVTFISSFSQAQTSMTQEEENMVKYWYYRNRLQ
                     YFMVQGAGIGREWGKRWGAN"
     gene            complement(286..786)
                     /locus_tag="PLK75_10355"
     CDS             complement(286..786)
                     /locus_tag="PLK75_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39689.1"
                     /translation="MKKTYILTAIILLIFLFGCSRYEDGPCISFRSPTSRVLGTWKVE
                     KLLINDLDSTELYRQQMGCEWEFTDDPFYENGDGYVLYYKNCMDDTVRKGLWYFDNIS
                     ENIIAIDMSGDIYSNPIGPLGRWEGWDILRLSNSEMHLWVKPEHYIIYWDYPENTTFY
                     LELKKL"
     gene            1093..1716
                     /locus_tag="PLK75_10360"
     CDS             1093..1716
                     /locus_tag="PLK75_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39690.1"
                     /translation="MLSNKLCLVFILASGLLSCGETKTTTETYPNGDLKMMGKEKYRK
                     ETYFSIKKEYYENEVLKSSERISPFGYRFYEYNKSGNLLRACTGRDVCSIIIYRCFNP
                     HAYTDGGSYHYKKQINSGDGNRYLEIKGKYLNAWSDSKHLEFRLSQFDTINSCRVITM
                     TLKKSTTGGIKIIKRQKGSKETLYFNNEKYFRQFVDSLYPTALGFQH"
     gene            complement(1757..3049)
                     /locus_tag="PLK75_10365"
     CDS             complement(1757..3049)
                     /locus_tag="PLK75_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOY39691.1"
                     /translation="MPESENKYSKALEIFTSQCDSKLLTHLNSCVHCGLCAESCIYYI
                     AMKEPKFMPGKKVDIVSSIYRRYCTFTGKVFPKLVGARDLDEPTINEMIDLLYGSCTM
                     CGRCTIHCSIGVDIAYVVRTGRNMLASMKMVPASLQSTVNAAVTSGNNMAIPTDEFVD
                     TIEWMEEELRSEDGCSEACIPLNQPDKNILYTLNPREPKFFPLSISAMAKIFHAAGES
                     WTLSTAMYDVTNYAFFSGDPEEAQIIARRLEGEMKNLNAKRCILGECGHGSRAFRWEA
                     PNYLHENFDFDVITSVELIYEYFKQGRIKLDKSKQTAMVTMHDPCNLVRNGGIIMQQR
                     EILRHCVTNFVETTPFGVDNYCCGGGGGQLAMSEYNERRMKIGEIKANQVKATGATIL
                     VTPCHNCVDQLIQLNSAFKMGVQIKTLAEIVADALVME"
     gene            complement(3053..3679)
                     /locus_tag="PLK75_10370"
     CDS             complement(3053..3679)
                     /locus_tag="PLK75_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39692.1"
                     /translation="MTLFAVQIASLVSLGVCLSFIVFHFIKLIRLGKPSDLSKSIGNE
                     KDGIIYSFTGAMSPTHKETAYLHLPTYTMGIIFHLATFLGIVVFFVKITGLYLPFTIA
                     AVISAAMTAGFVSGVIILIKRITLQRMRLLSNPDDYISNIVVDIFQFTTAIALVFEKF
                     DVVYYIVVCLLLLYFPLGKLRHAVYFFAARYHLGFFYGRRGVWPQKNK"
     gene            complement(3690..4007)
                     /locus_tag="PLK75_10375"
     CDS             complement(3690..4007)
                     /locus_tag="PLK75_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOY39693.1"
                     /translation="MAQLEIDGRIFEVDGDGFLQNPEIWNDDVARLFAKSDGIESMND
                     NHWAVVRVIRQNFEEKGMAPMVRVICKETGLKLKDIYELFPLGPARGACRIAGLPKPD
                     GCV"
     gene            complement(4019..4210)
                     /locus_tag="PLK75_10380"
     CDS             complement(4019..4210)
                     /locus_tag="PLK75_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39694.1"
                     /translation="MDDKIIQQNYISNYCKNSSGNWSNCKRLITKNEIHFCPDFVLPD
                     TVESVSEIIDIYDNSIDNN"
     gene            complement(4303..5175)
                     /gene="atpG"
                     /locus_tag="PLK75_10385"
     CDS             complement(4303..5175)
                     /gene="atpG"
                     /locus_tag="PLK75_10385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOY39695.1"
                     /translation="MSGLKELRSRISSVKSTRQITSAMKMVAAAKLKGAQDRIVAFRP
                     YAEKMQGIACSLSNTDNEKITSPYLLPSKSAKVLIVLFTSNRGLCGGFNNNIFKKAVE
                     WAYHNYQEAIKNNDLCFYCIGKKGGELIKRRGFKIDFVDNTIYEKISFANSNKIAEHL
                     MQLYSDGIYGKIDIAFNNFKNAGVQIQTIERFLPVSLPSGKQKPIDYLFEPSLKEIMS
                     ELIPKTLRVHFFKALQNSFASEQGARMTAMHQATDNASEIIKELTLQYNKARQAAITN
                     EILEITSGAEALKS"
     gene            complement(5184..6764)
                     /gene="atpA"
                     /locus_tag="PLK75_10390"
     CDS             complement(5184..6764)
                     /gene="atpA"
                     /locus_tag="PLK75_10390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOY39696.1"
                     /translation="MADIKPSEVTEILREQLQGIESKAGLEEVGTVLQVGDGIARIYG
                     LTKVQANELIEFSNGVQGIILNLEEDNVGAVLLGHSEEIKEGDIVKRLRRIASIKVGE
                     SMLGRVVNTLGQPLDGKGPISGELFEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMTP
                     IGRGQRELIIGDRQTGKTAIAVDTIINQKEFYEKGEPVYCIYVAIGQKGSTVAKIAKT
                     LEDAGAMPYTTIVCATASDPAAMQFYAPFAGAAIGEYFRDTGRPALIIYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDIFYLHSRLLERAAKIIEADEIAQQMNDLPACLQGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETGLFNSGIRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKIDQSQFRELEAFAKFGSDLDASTLAILDKGKKNVQILKQPQY
                     HPCKVEDQVAILFCGTNALLKAVPLAKVSEFENKYLSYLHLKHQDVLDQLKKGVFNDE
                     IASVLRSAAGEIADTYKN"
     gene            complement(6778..7332)
                     /gene="atpH"
                     /locus_tag="PLK75_10395"
     CDS             complement(6778..7332)
                     /gene="atpH"
                     /locus_tag="PLK75_10395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOY39697.1"
                     /translation="MNQSKISVRYAKALLLSAKEQNLLAPVYNDITSLFNLFSTSTDL
                     AEVFSNPVVKPSVKIKIVKELGRNTNAMVQSFMVMVINNGRENQFVQIFRNFISFYKA
                     EMGIKSAHVTSVFPIDKKNLENIRTILSEYFNCTIEIAQHSDPNLIGGFIIRIDTMQL
                     DKSVSSELKKIKRELTSETYKSKL"
     gene            complement(7334..7828)
                     /gene="atpF"
                     /locus_tag="PLK75_10400"
     CDS             complement(7334..7828)
                     /gene="atpF"
                     /locus_tag="PLK75_10400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOY39698.1"
                     /translation="MDLITPGIGLLFWMSISFILLVLILAKFAWKPILTSINKRNQSI
                     EDALMSAEKAREEMASLKSENEKILLQAKAQREEILKEARIFGEKIIADAKTSAKTDI
                     DKMKEQARLEITSEKTAALSQIKNQVAEISIQIAEKLLRKNLEEKERQEQLIEDILKE
                     VNLN"
     gene            complement(7905..8111)
                     /gene="atpE"
                     /locus_tag="PLK75_10405"
     CDS             complement(7905..8111)
                     /gene="atpE"
                     /locus_tag="PLK75_10405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOY39699.1"
                     /translation="MINGIAAIGAGLAVIGAGLGIGRIGGSAMDAIARQPEATSKIQL
                     AMIIAAALIEGVALFAVVVALMQG"
     gene            complement(8151..9239)
                     /gene="atpB"
                     /locus_tag="PLK75_10410"
     CDS             complement(8151..9239)
                     /gene="atpB"
                     /locus_tag="PLK75_10410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOY39700.1"
                     /translation="MRLLITYLLLLSISLGLNMNGFCSESHSGDDDFNTNELIMHHIK
                     DSHDWNLFSYSKNGEKHQVAIPLPIVLIYDGKFDVFMSSEFNHGNAVVTKGDRNYIMH
                     HEKIYLANDAGALDFEKSDNGKEDKILNNKPYDFSITRNVAALFLTIGILIWLFVGVA
                     KSYKKRPGRPAGIQAFLEPLILFVRDDIVLPNIGHRKYEKFLPYLLTVFFFILFSNVL
                     GLIPFFPGGSNVTGNIAVTFILALITLILTNINGNRSYWKHIFAAPGVPVWLYIVIIP
                     VELIGIISKPFALMIRLFANITAGHIVVLSLVSLIFIFKTLWISPVAVIFAVFMDVLE
                     LLVAFLQAYIFTILSALFIGMAVQEEHH"
     gene            complement(9301..>9568)
                     /locus_tag="PLK75_10415"
     CDS             complement(9301..>9568)
                     /locus_tag="PLK75_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39701.1"
                     /translation="TISGHIFLFFLTLINIICILWLFKKNITIMGYGFMLLGFVKMLL
                     SIIFLLPVLLHKNSATTGYVIQFLVVYVFYLVYEVIYVVRILKN"
BASE COUNT         2816 a         2006 c         1782 g         2964 t
ORIGIN      
        1 aagttataga tttgtgtcta taattttgtc tgatttttta aggggctaaa acactatcaa
       61 agttttgcta ttgtttttta tgcatgctaa tttgcacccc acctcttccc ccattctctc
      121 ccaattccag ctccctgaac cataaagtat tgtaggcggt tacggtaata ccagtatttt
      181 accatgtttt cttcttcctg tgtcatgctg gtttgtgctt gagaaaaaga ggagataaat
      241 gttactagta ttaaaatgta taatgtaata tataatttca ttttattaaa gtttttttaa
      301 ttcgagataa aatgtagtat tttcaggata atcccaataa ataatataat gctcaggttt
      361 aacccacaag tgcatttcgc tgttgcttag tcgtaatata tcccatcctt cccacctgcc
      421 taacgggccg attggattgg agtaaatatc acctgacata tcaatagcta ttatattttc
      481 tgaaatatta tcaaaatacc aaagtccttt gcgcactgta tcatccatac agtttttata
      541 atataaaaca tatccatctc cattttcgta aaatggatca tcggtaaact cccattcgca
      601 acccatttgc tggcgataca attcggtaga atccaagtcg tttatcaaca gtttttcgac
      661 tttccatgtt cccaataccc tgcttgttgg agaccggaaa ctgatgcacg gcccgtcttc
      721 gtagcgggaa cacccaaaga gaaaaatcag caagattatt gcggtaagga tgtatgtttt
      781 tttcatagct gtctggttta tttattaggg tcaatcttgt taagattggc ataacataca
      841 aatttaatca ttttgtttta tacttattaa tcaagcataa ttagtctgtt cttttccatt
      901 cccatccgtg taaatcggag actcgaatgt aaccgtattc ccgaatccct ttttaacaaa
      961 agcctacgcc ccaatggcgt tagccgtgca tcaattttgc tataattacc tgcaattttc
     1021 tccccaaacg tatatcttta gcattcgaaa tcgttatacg atgtactgtt gaatttatta
     1081 ttgcacaagt gtatgctgtc gaataaactt tgcctggttt ttatactagc ctcgggttta
     1141 ttatcctgtg gcgaaacgaa aaccaccacg gagacctatc caaatggtga tttgaaaatg
     1201 atgggaaaag agaaatacag aaaagagacg tattttagta tcaaaaaaga atattacgaa
     1261 aacgaagttt taaaatcatc tgaaaggatt tccccgtttg ggtatcgttt ctacgagtac
     1321 aacaaatcgg gtaacttact aagagcctgt accggacgag atgtctgtag cattattatt
     1381 tatcggtgtt ttaacccgca tgcttatact gatggggggt catatcatta taaaaagcaa
     1441 atcaattcgg gtgatggcaa tcgatacctc gaaataaagg gtaaatatct gaatgcctgg
     1501 agcgattcaa aacatttaga attcaggttg agtcaatttg atacgataaa tagttgtagg
     1561 gtaattacta tgaccctaaa gaagtcaaca accgggggca ttaaaattat caaacgacaa
     1621 aagggtagca aagagactct atattttaat aacgagaaat atttccggca atttgtagat
     1681 agcttgtacc caactgcctt aggttttcaa cactgaattt cctttttata ctggtattac
     1741 gataaatcct agatttttat tccataacca atgcatctgc cactatttcg gcaagtgttt
     1801 taatttgcac tcccatttta aatgcactgt tgagctggat caactgatca acacagttat
     1861 gacaaggtgt aaccaaaata gttgctccgg ttgcttttac ctgattggct tttatttcac
     1921 caattttcat tcggcgttca ttgtattcgc tcatagccag ttgcccacca cctccaccac
     1981 aacaataatt atccacgcca aaaggagtgg tttcaacaaa atttgtaacg caatgcctga
     2041 gaatttcgcg ttgctgcata attatcccgc cgtttcttac caggttgcag gggtcgtgca
     2101 tagtaaccat tgctgtttgt ttcgacttgt cgagcttgat tctcccttgt ttgaaatatt
     2161 cataaatcag ctccacagaa gtaatcacgt caaaatcgaa attttcgtga agatagtttg
     2221 gagcttccca gcgaaaggct cttgaaccgt gtccgcactc acctaaaata catcgttttg
     2281 cattcaggtt tttcatttct ccttccaagc gcctggcaat aatctgagct tcttcggggt
     2341 cacctgaaaa aaatgcataa ttcgtaacat catacattgc agtgcttaat gtccaacttt
     2401 cgccggcagc atggaatatt ttagccattg ccgaaattga aagcggaaag aacttgggtt
     2461 cccgcgggtt cagggtatac aatatatttt tatcgggttg attcaatgga atgcaagcct
     2521 cactgcagcc atcttcgcta cgaagttcct cttccatcca ctcgattgta tcaacaaatt
     2581 catcggttgg gatggccata ttattgccgg atgtaaccgc agcatttaca gttgattgca
     2641 acgatgcagg aaccattttc atactggcaa gcatattccg ccctgtgcga accacgtagg
     2701 caatatcaac accgatagaa cagtgaattg tacaacgtcc acacatagta cacgacccgt
     2761 acaacaaatc aatcatttca ttaattgtgg gttcatccag gtctcttgcg ccgacaagtt
     2821 tcgggaaaac cttgccggtg aaagtgcaat agcgccggta aatcgatgaa actatatcaa
     2881 cctttttgcc aggcataaac tttggctcct tcatggcgat atagtagata cagctttctg
     2941 cacataaccc gcagtgaaca catgaattca ggtgtgtaag caacttactg tcgcactggc
     3001 tggtgaaaat ttccagtgct tttgaatatt tgttttcaga ttcgggcatc ggttacttat
     3061 ttttttgagg ccatacaccc cttcttccgt aaaaaaatcc taaatgatag cgcgctgcaa
     3121 aaaagtaaac agcatgccgc aatttcccaa gtggaaaata caacaacagc aggcaaacaa
     3181 ctatataata aacaacgtcg aatttttcga acaccaatgc tatggctgtg gtaaactgaa
     3241 agatatcgac aacaatgttg gagatataat catcgggatt cgaaagcaat cgcatcctct
     3301 gcagggtgat tcgttttata aggatgataa ccccagaaac aaatcctgcg gtcattgctg
     3361 cagaaataac agccgcaatg gtaaaaggaa ggtacaatcc ggtaatttta acgaaaaaca
     3421 cgactattcc cagaaacgtt gccagatgaa aaataatccc catcgtatag gtaggaagat
     3481 gcaaataagc agtttcttta tgtgtcggac tcattgctcc agtaaaggag tagataattc
     3541 catctttttc gtttccgatg cttttcgaaa ggtcggaggg tttcccaagc cggatgagtt
     3601 ttataaaatg aaacacaata aaactcaggc agaccccaag cgatacaagc gatgcaatct
     3661 gaactgcaaa taatgtcata gtggtttgat taaacacatc catctggctt cggaagccct
     3721 gcaatgcgac aagccccacg tgccggcccg agagggaaaa gttcgtaaat atccttgagt
     3781 ttcagtccgg tctctttgca gattacacgc accatgggag ccattccctt ttcttcaaaa
     3841 ttctgccgga taacgcgaac tactgcccag tggttatcgt tcatagattc aatgccatcg
     3901 ctttttgcaa aaagcctagc aacatcatcg ttccaaattt caggattttg caagaatccg
     3961 tcaccatcaa cctcaaaaat tctcccatca atttccagtt gcgccatagt tatttgaatt
     4021 aattattatc aatgctatta tcgtatatgt caattatttc gctaacactt tcaacggtgt
     4081 cgggaagaac aaaatccgga cagaagtgga tttcgttttt tgtaataagt cttttacaat
     4141 tgctccagtt ccccgaagaa tttttgcaat aattgctgat gtaattctgc tgaataattt
     4201 tgtcatccaa aattgagttt ccggatatta acttgcaggt ttcaaaattt ggacaatgaa
     4261 tttccacttc aatttatttt gataataaaa cagctcactt cactaagatt taagggcttc
     4321 ggcaccgctt gttatttcaa ggatttcgtt ggtaatggca gcctgacggg ctttgttgta
     4381 ttgcagtgta agctctttaa taatctctga tgcattatcg gtagcttgat gcattgctgt
     4441 catccgggca ccttgctccg aagcgaaaga attctgcagt gctttaaaga aatgcacacg
     4501 aagtgtttta gggataagct ctgacataat ttccttcaga cttggttcaa acagataatc
     4561 gatgggtttt tgcttccccg aggggagaga cacaggtaaa aatctctcaa ttgtctgtat
     4621 ctgaacacct gcatttttaa aattattaaa agcgatatcg atttttccgt aaataccatc
     4681 tgaatacagt tgcatcaaat gttccgcgat tttgttggaa tttgcaaagc tgattttttc
     4741 gtagattgtg ttgtcgacaa agtcaatttt aaagcccctg cgcttaataa gttctccccc
     4801 tttttttccg atacagtaaa aacacaaatc attgttttta atagcttcct gataattatg
     4861 gtatgcccat tctaccgctt ttttaaaaat attgttgtta aagcctccgc acaatcccct
     4921 attggaggta aataaaacaa tcaacacttt tgctgatttc gatggcagca agtatgggct
     4981 cgtaattttt tcgttatctg tgttggaaag cgaacaggca ataccctgca ttttctcagc
     5041 ataaggtctg aaagcaacaa ttcggtcctg agctccttta agtttggctg cagccaccat
     5101 tttcatggca gaagtaatct gtcgtgtcga tttaaccgaa gatattcttg atcgcagctc
     5161 cttaagtcct gacataaagc atactagttt ttataagtat cagcaatttc acctgcagcc
     5221 gaccttagaa cagaggctat ttcatcgtta aaaacaccct ttttcaattg gtccagcaca
     5281 tcctggtgtt tcagatgcag atacgataag tacttgtttt cgaattcact gacttttgcc
     5341 agcggaacag ctttaagcaa tgcattggtg ccgcaaaaaa gaatggcaac ctgatcttcc
     5401 actttacagg gatgatattg gggttgctta agtatctgaa cattcttttt ccctttgtca
     5461 agaattgcca gtgttgaggc atcaaggtcg gagccgaatt tagcaaaagc ttccagttcg
     5521 cggaattgcg attggtctat ttttagtgtg ccggcaactt ttttcattga tttaatttgc
     5581 gcattacctc caacgcggga aaccgaaata cccacattta ttgcgggacg gattccggaa
     5641 ttgaataaac cggtttccag aaatatttga ccatcggtaa tcgaaattac attggtagga
     5701 atataggcag aaacgtcgcc tgcctgtgtt tcgatgattg gtagtgccgt aagcgatccc
     5761 cctcctttta cttttccttg aaggcaagcg ggtaagtcgt tcatttgctg ggcaatttca
     5821 tcagcttcaa taatttttgc agctctttcg agtaggcgtg agtgcagata aaaaatatca
     5881 ccgggataag cttcacgacc aggaggcctg cgaagcagca acgatacttc acggtacgaa
     5941 acagcctgtt tcgacaggtc atcgtaaatg attagtgcag gacgcccggt atcacgaaag
     6001 tattccccta ttgcagcacc tgcaaaaggt gcataaaact gcattgctgc aggatcggaa
     6061 gctgtggcac aaacaatggt agtgtagggc attgctcctg catcttcgag ggttttagca
     6121 attttagcaa cggtagatcc tttttgtcca attgcgacat aaatacagta taccggttcg
     6181 cctttctcgt agaattcttt ttgattgata atggtatcga ccgcaatagc ggttttccca
     6241 gtttgcctgt cgccaataat gagctcgcgt tgaccccttc ctatcggagt catggcatcg
     6301 attgctttaa taccggtctg caaaggctcg ttaacaggtt gccggaaaat aactccgggt
     6361 gcttttcgtt ccaggggcat ttcgaagagc tcaccactaa tcggaccttt cccatcaagc
     6421 ggttgcccca gggtattcac cacacgcccc aacattgatt ctccaacctt gatagaagca
     6481 attctgcgaa gtcgtttcac aatatcgcct tcctttattt cttcgctatg gcccagcaaa
     6541 acagccccaa cattatcttc ttcaaggttt agaataatgc cctgaacacc attgctgaat
     6601 tcgatcaatt cgtttgcctg aaccttggtt aaaccataaa ttcttgcaat accatcgcct
     6661 acttgcaaca ctgtgcctac ttcttcaagg ccagctttac tttcgatgcc ctgcagttgc
     6721 tcacgcaaaa tttcagttac ctctgatggt tttatatcag ccattttttt aaattaatta
     6781 tagtttactt ttatacgttt ctgatgtcag ttcgcgcttg atttttttca actcggaact
     6841 tacactttta tcgagttgca tagtatcgat acgaattata aaacccccga tcagatttgg
     6901 atcggaatgc tgcgcaattt cgatagtgca gttgaaatat tcagataaaa tagttctgat
     6961 attttcgagg ttttttttgt cgataggaaa tacgctggta acatgagcag attttattcc
     7021 catttcagct ttatagaacg aaataaaatt cctgaagatc tgaacaaatt ggttttcacg
     7081 gccgttattg atgaccatca ccataaaact ttgaaccatt gcatttgtgt tccgccccag
     7141 ttctttcact attttaattt taaccgatgg ttttaccaca gggtttgaaa aaacctcggc
     7201 aagatcggtg cttgtgctga acagattgaa caaagatgta atgtcattgt aaaccggtgc
     7261 caacaggttt tgttctttgg cagacagcag caaggctttt gcgtaacgga ccgatatttt
     7321 actctgattc atgttagttc agattcactt ctttaagtat atcttcaata agttgttcct
     7381 gccgttcttt ttcttcaaga ttctttctaa ggagcttctc tgctatttgg atggagattt
     7441 cggcaacctg atttttaatc tgacttaatg cagctgtttt ctcactggta atttccaacc
     7501 gtgcctgttc cttcatttta tcgatatctg ttttagcact ggttttagcg tcagcgataa
     7561 ttttttcgcc aaatattctg gcttctttca gtatttcttc acgttgtgct ttagcctgaa
     7621 gcagaatttt ttcgttttcc gatttcaggc tggccatttc ttctctagct ttttcggctg
     7681 acattagcgc atcttcaatc gactgatttc gcttgtttat tgaagtaagg atcggcttcc
     7741 aagcaaattt tgccagaata agaacaagca aaatgaagga aatgctcatc cagaacaaca
     7801 atccgattcc gggggtaatt aaatccataa gttcttattt taatggtaca ttaaaaaatg
     7861 taaagtaacc ctggtgccaa ccgcctacag ggttactaag tttgttaccc ttgcataagt
     7921 gcaacaacaa ctgcaaaaag ggcaacccct tcgatgagtg ctgctgcaat aatcattgca
     7981 agctgaattt tacttgtggc ttctggttgg cgtgcaatag catccattgc tgatccgcca
     8041 attcttccaa tgcccagtcc ggctccaatt actgccagac ctgctccgat tgctgcgatt
     8101 ccgttaatca taattataat ataaaattaa acataaaatt caatataact ttaatgatgc
     8161 tcttcctgta cagccattcc aataaataat gccgacagga tagtaaatat atatgcctgt
     8221 aagaaagcca ccagcaactc caacacatcc ataaacactg cgaaaattac tgctacaggt
     8281 gaaatccaca aggttttgaa aataaatatc aatgaaacaa gacttaatac gacaatatga
     8341 ccagcggtaa tgtttgcaaa caaacgaatc ataagtgcaa aaggcttgct gataattcca
     8401 ataagttcaa cgggtattat tacaatgtaa agccacaccg ggacccccgg agcagcaaat
     8461 atatgtttcc agtagctgcg gttcccgtta atattggtta aaatcaatgt tattaacgca
     8521 aggatgaacg taacagcaat gttccctgtg acatttgacc caccgggaaa aaatggtatt
     8581 aatcctagaa cgttgctgaa aagaatgaaa aagaaaacgg taagcaaata cggtaaaaac
     8641 ttctcgtatt tgcgatgccc gatatttggc agaacaatgt cgtcgcgtac aaatagaatt
     8701 aatggttcta aaaatgcctg tatgccagcc ggacgtccgg gtcttttctt gtacgatttc
     8761 gcaacaccaa caaaaagcca tattaaaatt ccaattgtca aaaacaatgc agcaacattt
     8821 cgggtaatcg agaagtcgta tggtttattg ttaagaattt tgtcttcttt tccattgtct
     8881 gatttttcaa agtcgagtgc cccggcatcg ttggcaaggt aaatcttttc gtgatgcatt
     8941 atataatttc gatcaccctt ggtcaccact gcgttaccgt gattaaactc cgacgacatg
     9001 aaaacgtcaa atttgccatc atatatcagt acaatcggaa gtggaattgc cacctgatgc
     9061 ttttctccgt tttttgaata gctgaacaga ttccaatcat gcgaatcttt aatatgatgc
     9121 atgattaatt cattggtatt aaaatcgtca tccccggaat ggctttcact gcagaaccca
     9181 ttcatattca agcccaaaga tatactcagg agcagtaaat atgttattaa taaacgcatc
     9241 agaaaactct ttttttcagg tcttgaaaat acacattttt tattaaatga agactctgaa
     9301 ttaatttttt agtattctta caacataaat tacctcataa actaggtaaa aaacataaac
     9361 aacgagaaac tgtatcacat agcctgttgt tgcactgttt ttatgcagca gaacaggcaa
     9421 aagaaaaata atggatagca gcatcttaac aaaaccgagc aacatgaacc catatcccat
     9481 gatggttatg tttttcttaa aaagccacaa aatacaaatg atgtttatca gagtaaggaa
     9541 aaataaaaaa atgtgaccag atatggtt
//
LOCUS       DAOOQP010000112         9555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8883_length_9555_cov_4.796421, whole genome shotgun sequence.
ACCESSION   DAOOQP010000112 DAOOQP010000000
VERSION     DAOOQP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8883_length_9555_cov_4.796421"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLK75_10420"
     CDS             <1..484
                     /locus_tag="PLK75_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39702.1"
                     /translation="IAVFFLQGFLSVAQIYRNGLGVRIGETQGIIYKQFFAKTVALEA
                     GLAFFKQGVNLTLIAENHHFDMFRTKNLYLFYGFGLHAGYFDGTRIPLWFPDDRGFRY
                     YFVSGVDGIVGMEYTFEQIPVCIGLELKPAMNIIGTVSYWQGYAVSLKYIFYNIKPLQ
                     "
     gene            487..1611
                     /gene="dprA"
                     /locus_tag="PLK75_10425"
     CDS             487..1611
                     /gene="dprA"
                     /locus_tag="PLK75_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOY39703.1"
                     /translation="MEQDIYKLAISRVPGMGCITARKIVSIVHDLEVVFLEKASVLKR
                     ISGIGDFAAGKIARNRQSALELAHQELEYCVRNEISFISFIDKGYPLQLQECEDAPLL
                     LYYKGCPDFGNVKNISVVGTRNVTRYGSENCNKIVSQIAARHPEAVIISGLALGVDGI
                     AHTAALKSGLKTWAVIGHGFETIYPGEHYGLAADILEKEGAIITEYPHNSKIRPSNFV
                     ERNRIVAGLAKAVLVVESAKKGGAMITADLANQYNRDVFALPGAVDRMGGNGCNLLIK
                     THRAHLCEDVEDIEYITGWKPNAETKNCQSIEFPDDGRFSEQELLILEALKKEQYNDF
                     DGLLTKTGLTTGQISTSLLELEFRGIIRSLPGKIFSLRKS"
     gene            1808..3154
                     /gene="gdhA"
                     /locus_tag="PLK75_10430"
     CDS             1808..3154
                     /gene="gdhA"
                     /locus_tag="PLK75_10430"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOY39704.1"
                     /translation="MEAQVKLFMEKVKAQNPGQPEFIQAVQEVAEKLIPFIEENPKYK
                     DAKILERMVEPERTIIFRVPWVDDEGEIQVNRGYRIEFNSAIGPYKGGIRFHPSVTLS
                     ILKFLGFEQVFKNSLTTLPMGGGKGGSDFNPKGKSDAEVMRFCQSFMMELFRHIGPDT
                     DVPAGDIGVGGREIGYMFGMYKKLRNEFTGVLTGKGIAWGGSLARPEATGFGNVYFAQ
                     NMLQTRGETFKGKDVAVSGFGNVAWGACKKVTDLGGKVVTISGPDGYIFDPDGINTEE
                     KFDYMLELRASNNDVVAPYAERFPKATFHPGKRPWEQKVKVALPCATQNELNLEDAKH
                     LIANGCICVSEGANMPCTPEAVEAFQEAKLLFAPGKAANAGGVATSGLEMTQNSMRLS
                     WTFEKVDEELKHIMKSIHDTCVKFGKNKEGYIDYVKGANIGGFVKVADAMIAQGVV"
     gene            3238..4002
                     /locus_tag="PLK75_10435"
     CDS             3238..4002
                     /locus_tag="PLK75_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOY39705.1"
                     /translation="MFVDTHTHIYLEQFDNDRDDVVHNAIQTGVTRMLLPNINSETVL
                     PMMSLCKQYPKHIFPMIGLHPTSVTPDYHKELINVEKELNQHKYIAIGEVGIDLYWDD
                     TYIQEQKQVFDYQIGIALQYHLPVVVHMRNSFNEIWDVLQPYKNKNLKLVMHCFSGDR
                     VQAEKIVDFGYYIGIGGVLTYKNSLISEIANYIPLENIVLETDAPYLAPVPYRGKRNE
                     PKYIQIINNRLAEIRKADVHAVKLQTTQNAIDLFNL"
     gene            4113..4200
                     /locus_tag="PLK75_10440"
     tRNA            4113..4200
                     /locus_tag="PLK75_10440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4147..4149,aa:Ser,seq:gga)
     gene            4263..5087
                     /locus_tag="PLK75_10445"
     CDS             4263..5087
                     /locus_tag="PLK75_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOY39706.1"
                     /translation="MKRILAVFFAASLLTLSATSFVMGQTKDENKPTEATQQVADSNV
                     TTATEAAADVEEPAPTPEVPMHQQLKIKFIEGGPMWMAPILICLIIGLALAFERILYL
                     NFASTNTKKLLRNVENALNNGGVDAAKEVCRNTRGPVASIFYQGLDRYHAGIDIVEKS
                     VISYGSVQMGLLEKGLTWIALFIALAPMLGFLGTVVGMVQAFDAIEAAGDINPTLVAG
                     GMKVALITTIGGLVVAMVLQVFYNYIIGKIDSIVNNMEDASISLIDILVEYKNKNA"
     gene            5090..5596
                     /locus_tag="PLK75_10450"
     CDS             5090..5596
                     /locus_tag="PLK75_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39707.1"
                     /translation="MVEKIIKYLGWAVFALTAILSLLFFFKDYPGLSTEISKIEGLTS
                     VDKQIATAQIASNWSATLLNFAGIMLFVTIGLVIVFALYAFIANFLIEPKKAIRSLMS
                     ILAIGAVVVVAYILASDAIPVFIGSEKLEITPRLSKWVDTSLYTTYIVFGAALIMTLY
                     SEVSKIWK"
     gene            5634..6215
                     /locus_tag="PLK75_10455"
     CDS             5634..6215
                     /locus_tag="PLK75_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOY39708.1"
                     /translation="MARKTPEVNSGSTADIAFLLLIFFLLTSTMDVDTGIARKLPPMP
                     DKTVQETKVDIKERNVCVVLINAQDRILFRNKPVAATDVRELAKEFIKNDRDEEDLPE
                     KEPVYVKELNKTVNVTKGVISLQNDRGTSYGMYITVQNELVAAYNEVRDEAAMLYFGI
                     RKFDDLKEYQQEAIKKIYPQRISEAEPKNIGGK"
     gene            6217..6690
                     /locus_tag="PLK75_10460"
     CDS             6217..6690
                     /locus_tag="PLK75_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOY39709.1"
                     /translation="MAKFRKGEKKEAPGISTSSLPDIVFMMLFFFMVISSMREVELKI
                     EVNAATLPAATEIKKLEKKSLVSFIYVGKPKVQFQNRYGTEPMIQLNNSFASVSDIRD
                     YIIAEREQRDESEIPYMTTSLKVHRDTKMGIITDIKQELRKASALKINYSARKVI"
     gene            6885..>9555
                     /locus_tag="PLK75_10465"
     CDS             6885..>9555
                     /locus_tag="PLK75_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39710.1"
                     /translation="MKKIILVPLVLIIALVNPVIAQTYLISSGGTVNTCSGTFYDSGG
                     AGGQYGNSQNYTMTICSNLPGAHIVVHFTSFQLESNFDYLSIYNGPTTGSPVLVNNGS
                     GTTLNGNTYTSTGTCLTFRFTSDGSVTYSGWSATISCSFPCQDYSVAITSTNPPVSNA
                     DSLFVDICQGDVASFTAQGSYPNSGVNYTQSDATTEFVWMVVDGSTTTEYPGVGMTTF
                     THTFTQAGGYFVNLVTYDPVDCTATLVDQYRVRVSLTPNFENVGSDVSIICPGDPVNL
                     TGTVTSNTWTMPVTETQIVQECFYDNYEGTVCFTVSAFQPGQVITSMYDLESVCFNME
                     HSFIGDFYMYIVCPNGQEAMLHEYYNCNGAYFGIPDQSDNCNPGTGYTYCWSMSASQP
                     VTSICSSGSTVPAGTYLPLGTFEDLIGCPINGDWCVRFFDNWGADDGTIFYADLDFAD
                     NIIPNTLWAITNTFNVSPTSTDLVWSGDGVEPNTGGNALAHPTTPGDQPFTFTVTDEY
                     GCEYDTTIYVTVRQPNDPMCCVMPDPDAGPDELVCTNTYTFNPTVTVGNTYNWTMVSG
                     PGTAVWTNQTSANASVTVSEYGVYVFQITEQNLSPTCSVSDQVEISLWPVPTSTFAAS
                     AVLCEGEISTITYTGSATAAASFTWDFAGGTVSSGSGIGPFHVTWSTAGAHLMSLQVT
                     EHTCSSVVTTLSISTPALLDANPTFTDDPCYHSCNGEATLNVIGGTPPYEFSWASSTN
                     YQNHLCAGDYVVSVTDVNECEDVVNFTIAEPTELIITDTSFSNISCYQADDGEMHITV
                     SGGVLPYTYVWSDFGPGVPDRTNVPAGNYYVTVYDSNGCNVVEFYQLTQPAQLQVTIP
                     NSMSICEGQAITLTAQAMGGTMPYTYYWNSGGGF"
BASE COUNT         2808 a         1788 c         2133 g         2826 t
ORIGIN      
        1 aattgctgtt ttttttctgc aagggttctt gtcggttgca cagatctacc gtaatggttt
       61 aggtgtgaga attggggaga cacagggtat tatttataag cagttttttg ctaaaactgt
      121 agcgcttgaa gccggtctgg cctttttcaa acagggggtg aatttaaccc tcattgccga
      181 aaatcatcat ttcgatatgt tccgcacaaa aaacctttac ctgttttatg gctttgggtt
      241 gcatgcaggc tatttcgatg gaacaagaat cccgctatgg ttcccggatg atcggggttt
      301 tagatattat tttgtgtcgg gagtagatgg cattgtgggc atggaataca cattcgaaca
      361 gatacctgtt tgtattgggc ttgaattgaa acctgcaatg aatattatag gtaccgtatc
      421 gtactggcag ggctatgcgg tgtcgctgaa gtacatattt tataacataa aaccattaca
      481 gtagggttgg aacaggatat ttacaaactt gcaataagca gggttcctgg catgggatgt
      541 attactgcga gaaaaattgt ctccatagtt cacgatctgg aagtggtgtt tctcgaaaaa
      601 gcctctgtgc taaagagaat ttcaggcatt ggcgactttg ctgccggaaa gattgccaga
      661 aaccggcaaa gcgctctcga gcttgctcat caggagcttg agtactgtgt ccgcaacgaa
      721 atatcattta tttcttttat cgataagggg tatccgttac aattgcagga atgcgaagat
      781 gcaccattgt tgttgtatta taaaggatgt ccagattttg ggaatgtaaa aaatattagc
      841 gttgtgggca ctaggaatgt aacacgatat gggtcggaga actgcaataa aattgtgtcg
      901 cagattgctg cgaggcaccc agaggctgtt attatcagtg gtcttgctct tggtgttgat
      961 ggtattgcac atacagctgc gctgaaatct ggattgaaaa catgggctgt tattggtcat
     1021 ggttttgaaa ccatttaccc aggcgaacat tatgggttgg ctgctgacat tttggagaaa
     1081 gaaggcgcta taattaccga gtatccccat aacagtaaaa ttcgaccatc aaattttgtc
     1141 gagcgcaacc ggatagttgc aggtttggca aaagcagtgc tggttgttga gtcggccaaa
     1201 aaaggaggag caatgattac tgccgatctg gcgaatcagt acaacaggga tgttttcgcc
     1261 ttaccaggcg ctgttgaccg gatgggtgga aatggttgca acctgctgat aaaaacccat
     1321 cgggctcatt tgtgtgaaga tgtcgaagac attgaataca ttacggggtg gaagccaaat
     1381 gcagaaacaa aaaattgtca aagtattgaa ttccctgatg acggtaggtt tagtgaacag
     1441 gaattactga tactcgaagc attgaaaaag gaacagtaca atgatttcga cggattgctt
     1501 acaaaaacag gacttacaac cgggcaaatc agtacttctt tgctcgagtt agaattcaga
     1561 ggtataatcc ggtcattgcc cggtaaaata ttttccctgc ggaaatcata aaattatgac
     1621 attaatcata taatgctgta aaacagattt gcatgaggca taaccatcag aaaatcagtc
     1681 ttttgatgta attttcgcaa acgaataatc aaggcaaaaa gagcgtattg ttgttaaata
     1741 gttgtattat tgcaaactat ttcgaatttg taagattaag tacctaaaac tataaacaaa
     1801 caaaagcatg gaagcacaag taaaattatt catggagaag gttaaagccc agaatccggg
     1861 acaacctgag tttattcaag cagttcagga agtagctgaa aagctgattc cttttattga
     1921 agaaaatccg aaatacaagg atgcaaaaat cctcgagcgt atggtagaac ccgaaagaac
     1981 tattattttc agagttccct gggttgatga tgaaggagaa attcaggtta accgtggata
     2041 ccgcatcgag ttcaatagtg ctattggccc atacaaaggc gggattcgtt ttcacccgag
     2101 tgtaacttta tcaatactga agtttttagg ttttgagcaa gttttcaaaa attcacttac
     2161 aactcttcct atgggtggtg gtaaaggtgg atccgatttc aaccctaagg gaaaatctga
     2221 tgctgaagtt atgcgttttt gtcagagttt tatgatggaa ttatttcgtc atattggtcc
     2281 ggatactgat gttcccgctg gtgatatcgg tgttggtggc cgcgaaatag gttatatgtt
     2341 tggcatgtac aaaaaactgc gtaatgaatt caccggagtg cttacaggta aaggcattgc
     2401 atggggtggt tcgctggcac gtccggaagc tacaggcttt ggaaacgtat actttgcaca
     2461 gaatatgctt caaaccagag gtgaaacatt taaaggaaaa gatgttgcag tatcaggatt
     2521 tggtaatgtg gcatggggtg catgcaaaaa agtaaccgat ttgggtggta aagttgttac
     2581 catttcgggc cccgatggtt atattttcga tccagacgga atcaatactg aagaaaaatt
     2641 cgactatatg cttgagttac gtgcttcgaa caacgatgtt gtagctcctt atgctgaaag
     2701 attccccaaa gctactttcc atcccggcaa acgcccgtgg gaacagaaag tgaaagttgc
     2761 tctcccatgt gcaactcaga acgaactgaa tctcgaagat gcaaaacacc ttatcgccaa
     2821 tggttgtatt tgtgtttctg aaggtgccaa tatgccttgt acccccgaag cagttgaagc
     2881 tttccaggag gccaaactgt tatttgcacc cggaaaagca gccaatgccg gtggtgttgc
     2941 tacttcagga cttgaaatga cacaaaattc aatgcgtctt tcatggactt tcgaaaaagt
     3001 tgatgaagaa ctgaaacaca tcatgaaaag cattcacgat acttgcgtaa aatttggaaa
     3061 aaacaaggaa ggttacatcg attatgtaaa aggtgcaaac atcggtggtt ttgtaaaagt
     3121 tgccgatgca atgattgctc agggagttgt ttaataatta agttatattt tgttttcgaa
     3181 aagccttcaa ttgagggctt ttcttatttt tgtaatcttt tactttatta aaacgcaatg
     3241 tttgttgata cacatactca tatatatctg gagcaattcg acaacgacag ggacgacgta
     3301 gtgcataatg ccattcaaac tggtgtaacc cgaatgcttc tcccaaatat caacagtgag
     3361 acagtgcttc caatgatgag tttatgcaag caatatccaa aacacatttt tccaatgata
     3421 gggcttcatc ctacttcagt aacacccgat taccataaag aactcataaa tgttgaaaaa
     3481 gagcttaacc aacataagta tattgcaata ggggaggttg gaatcgattt gtattgggat
     3541 gatacatata tacaagaaca aaaacaggtt ttcgattacc agatcggcat tgctttgcag
     3601 tatcaccttc cggttgtcgt tcacatgagg aattcattca atgagatatg ggatgttttg
     3661 caaccttaca agaataaaaa cctgaaactc gtcatgcatt gcttctctgg agaccgtgtt
     3721 caggctgaaa agattgtaga ttttggttac tatattggta ttggcggtgt gttgacgtac
     3781 aagaacagtc ttatctccga aattgccaat tatattcctc ttgaaaacat tgtgcttgaa
     3841 acagatgcac catacctggc tcctgtacca tatcggggca aacgcaatga accaaaatat
     3901 attcaaatta ttaacaaccg acttgctgaa atccgtaagg cagatgtcca tgcggttaaa
     3961 ttacaaacta cacaaaatgc tattgattta ttcaatttat aagcgttggt cgaatatgtt
     4021 ggccgtgggt taattattgg taaataatca taaaatattt tgttgtcaat ttaaaaattt
     4081 attattttgc gtggcatttt gagagagtga tgggagagat ggccgagtgg tcgaaggcgc
     4141 acgcctggaa agtgtgtatg ccccaaaagg gcatcgtggg ttcgaatccc actctctccg
     4201 ctagtatcga attacattga tttatttaaa atagtgatag ttaatttaaa ccaaaaatga
     4261 aaatgaaaag aatcttagcg gtattttttg cagcaagttt gctgactctt tcagcaacat
     4321 cttttgtaat gggtcaaact aaagatgaaa ataaaccaac cgaagccact cagcaagtag
     4381 cagactccaa tgtcacgaca gcaacggaag ccgctgcaga tgttgaagaa cctgctccca
     4441 cgccggaagt cccgatgcat cagcagttga agattaagtt tattgagggt ggtccgatgt
     4501 ggatggcacc aatccttatc tgtcttatta ttggtttggc cctagcattt gagcgcatac
     4561 tctatcttaa ttttgcctca acaaatacta agaaactgtt gagaaatgtc gaaaatgcac
     4621 tgaataacgg aggtgttgat gcagcaaaag aagtttgccg taacaccagg ggccctgtgg
     4681 ctagtatatt ctatcaggga ctcgacaggt atcacgctgg tattgatatc gttgagaaat
     4741 cagttatttc ttacggaagt gttcaaatgg gtttgctcga aaaagggctc acttggattg
     4801 ccttgtttat tgctttggca cctatgttag gattccttgg tacggtagta ggtatggttc
     4861 aggctttcga tgctattgaa gctgccggtg atatcaaccc gactctggtt gccggaggta
     4921 tgaaagttgc tcttatcacc actatcggag gtcttgttgt tgccatggtt cttcaggttt
     4981 tctacaatta tatcattggc aaaatcgatt ctattgtaaa taacatggaa gatgcctcta
     5041 tttctttaat agatattttg gttgaataca aaaataaaaa tgcataatca tggttgagaa
     5101 aataattaaa tatttgggat gggcagtctt tgcattgact gctatcctca gcctgctctt
     5161 cttttttaaa gattatccgg ggcttagcac tgaaatctcg aaaatagaag gacttacatc
     5221 tgttgacaaa cagattgcca cggcccaaat tgcttccaat tggagcgcta cattgttgaa
     5281 ttttgcagga attatgcttt ttgttactat cggtttggtg atagtttttg ctttatatgc
     5341 ttttattgcc aattttttaa ttgaaccgaa gaaggccata cgaagcctga tgtcgatact
     5401 ggctattggc gcagtggttg ttgttgcata catacttgct tctgatgcta ttcctgtgtt
     5461 tatcgggtca gagaaactgg aaatcacccc gaggttatca aaatgggttg acaccagctt
     5521 gtatacaaca tacatcgtgt tcggagcagc gttaataatg acattgtatt cagaagtatc
     5581 gaaaatctgg aaataaggaa ttcgaattta tattaatatc aaattaattt attatggcta
     5641 gaaaaacgcc tgaagttaat agtggatcaa cagccgatat tgcattcctg cttttgattt
     5701 tctttctact gacctcgaca atggatgttg atacaggtat tgcaagaaaa cttccaccta
     5761 tgcctgacaa aaccgtacaa gagacaaaag tagatattaa ggaacgtaat gtttgtgttg
     5821 ttttgataaa tgcacaggat cgtattttat tcagaaataa accggtagcc gcaacggatg
     5881 ttcgtgaatt ggcaaaagaa ttcattaaaa acgaccgcga tgaagaggat ttgcctgaaa
     5941 aagaacccgt atatgtgaag gaattaaata aaacagtaaa tgttacaaaa ggcgtaatat
     6001 ctttgcaaaa cgacaggggt acgtcgtatg ggatgtatat tactgtgcag aatgaattgg
     6061 tggctgcata taacgaggtt agggatgagg cggctatgct gtattttggt atcagaaaat
     6121 tcgatgacct gaaagaatat cagcaagaag caattaagaa aatttatccc cagcgcattt
     6181 cggaagcaga acctaaaaac ataggaggca aataaaatgg caaaattcag aaaaggtgaa
     6241 aagaaagaag ccccgggaat ttcgacttcc tcgctacccg atattgtatt tatgatgctt
     6301 ttcttcttta tggtaatttc ctcaatgaga gaagttgagc ttaaaatcga agttaatgcc
     6361 gcaactttac ctgctgctac cgagattaaa aaactggaga aaaaatccct cgtaagcttt
     6421 atttatgttg gaaaaccaaa agtccaattc cagaacagat atggaacaga accaatgatc
     6481 cagttgaata attcctttgc atcggtatcc gatatccgcg attacattat tgccgaaaga
     6541 gagcaacgcg atgaatcgga aatcccctat atgactactt ctcttaaagt acacagagat
     6601 acaaaaatgg gcattatcac tgatattaaa caagaattgc gcaaagccag tgctttgaaa
     6661 atcaattatt cggcacgaaa agtgatttaa tgcaaatcga tgatttctta aaaacgggaa
     6721 gtgtttcaca ttcccgtttt ttttttatct tagtacattt tatgtagcaa acctttttga
     6781 attaaaaacg tcatcatgat tgtagatgcc gctgtaaaaa tgatattaat aaatatgcca
     6841 aaaggttttt caacggtttg aaagcgagat ataataatgt tgttatgaaa aaaattattt
     6901 tagttccttt agtcctgata atagccctgg tgaatcctgt gattgcacaa acctatctta
     6961 tcagcagtgg aggcacagtt aatacctgtt cgggtacgtt ttacgattca ggtggggctg
     7021 gtgggcaata tggtaatagt caaaattata cgatgactat ttgcagcaat ttaccgggtg
     7081 cacatattgt tgtccacttc acatcttttc agcttgagtc gaatttcgat tacctgtcta
     7141 tttataacgg accaacaaca ggcagccctg ttctcgttaa caatggatct ggtacaaccc
     7201 tcaatggcaa tacctatact tctaccggca cttgtcttac attccggttt acatccgatg
     7261 gctctgtgac ttactcgggt tggtctgcaa caatcagctg cagttttccc tgccaggatt
     7321 actcggttgc cattacttcg acaaatcctc ctgtgagcaa tgccgattcg ctgtttgtgg
     7381 atatttgtca gggcgatgtt gctagtttta cagctcaggg atcttatccg aatagtgggg
     7441 taaattatac gcagagcgat gcgaccaccg aatttgtctg gatggttgtt gacggaagca
     7501 caacaaccga atatcccggc gtgggcatga caacgtttac tcacaccttt acccaagcgg
     7561 gcggatattt tgttaatctt gttacttacg accctgttga ttgcaccgca acgcttgtcg
     7621 atcaatatag ggtcagggtt tcattaaccc ctaactttga gaatgtcggt agcgatgtca
     7681 gtattatttg ccccggagac cctgtgaacc ttaccggaac tgtaacatca aatacatgga
     7741 cgatgcctgt tacagagact cagatagtgc aagagtgctt ctacgataat tatgagggaa
     7801 ccgtttgttt cactgtaagt gctttccagc ccggacaggt gattacttca atgtatgatc
     7861 tggaatcggt ttgttttaat atggagcatt cttttattgg tgacttctat atgtacattg
     7921 tttgtccgaa cgggcaggag gcaatgcttc atgaatatta taactgtaac ggtgcatact
     7981 ttggaatacc tgatcagtct gacaattgca acccgggcac aggatataca tactgctggt
     8041 cgatgtctgc ttctcagccc gtaacatcaa tatgttcatc aggatctaca gtgccagctg
     8101 gaacatatct cccgttgggt acattcgaag acctcatcgg atgccccata aatggtgact
     8161 ggtgtgtgcg attcttcgat aactggggtg cagatgacgg tactattttt tatgcagacc
     8221 ttgatttcgc agataatatt atacccaata ctctttgggc tattacgaat acttttaatg
     8281 taagtcccac atcaaccgac ttggtttggt caggggatgg ggttgagcca aataccggag
     8341 ggaatgctct tgcgcatcct acaactcccg gagaccaacc gtttaccttt actgttaccg
     8401 atgaatatgg ttgtgagtac gatacaacta tttatgtaac tgtgcgtcag cctaacgacc
     8461 caatgtgctg tgtaatgcca gaccccgatg cagggcccga tgaactggtt tgcacaaaca
     8521 cgtatacttt taacccaaca gtaactgtgg gcaatactta taattggaca atggtcagtg
     8581 gtcctggcac agctgtatgg acaaaccaga catcagccaa tgcttcggtt acagtttctg
     8641 agtatggagt atatgtgttc caaataacgg aacaaaacct ctccccaaca tgcagcgttt
     8701 ccgatcaggt agaaatttct ttatggccgg tcccgacaag tacgtttgct gcaagtgctg
     8761 tgctttgcga aggcgagatt tcgacaatca cttatacagg aagtgctact gctgctgcga
     8821 gcttcacctg ggattttgcg ggtggtacag tgtcttcagg ttcaggtatt ggaccgtttc
     8881 acgtaacatg gagcacagct ggagcacatc tgatgagctt gcaggttact gaacatactt
     8941 gttcctcagt ggtaacaacg ctaagcattt cgacacctgc attacttgat gccaatccaa
     9001 cttttaccga cgatccctgt tatcattctt gcaatggaga agcaacactt aatgttatag
     9061 gagggacacc cccttatgaa ttttcgtggg catcttcaac aaattaccag aaccatcttt
     9121 gtgccggaga ttatgttgtt tcggttaccg atgtaaatga atgtgaagat gttgtgaatt
     9181 ttaccattgc tgaaccaaca gaattgatta tcaccgatac tagtttttca aatatttcgt
     9241 gttatcaggc cgatgatggc gagatgcata ttaccgtctc cggaggcgta ctgccttata
     9301 cttatgtatg gagcgacttt ggtccgggag tgcctgatcg tacaaatgtc ccagccggaa
     9361 attactatgt tactgtttac gattcaaatg gatgcaacgt tgttgaattt taccagttga
     9421 ctcagccggc tcaattgcag gttactattc cgaatagcat gtcgatttgt gaaggacagg
     9481 ccataacgct tactgctcag gccatgggtg gaacaatgcc ctatacgtat tactggaact
     9541 cgggtggcgg ttttt
//
LOCUS       DAOOQP010000113         9480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_8966_length_9480_cov_5.322971, whole genome shotgun sequence.
ACCESSION   DAOOQP010000113 DAOOQP010000000
VERSION     DAOOQP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8966_length_9480_cov_5.322971"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="PLK75_10470"
     CDS             complement(<1..1979)
                     /locus_tag="PLK75_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY39711.1"
                     /translation="MKRTLLLIALCFSILGVFSQTQITLKDIYGAGTFYPNTVDGIVS
                     MNDGEHYTTLNMGRNTISKYSYKTGEMVENLFKAKDFKDVKIPWIFDYQFNHDETMML
                     ISTNYDPIYRHSFKADYYIYDLKEKTLKPLSEEGSQQLACFSPDGSKISFVRNNNIYV
                     VDLATGIEQSITTDGEWNHIINGAPDWVYEEEFSFSKAYEWSPDGNYLAFLKTDESNV
                     KQYSIQLFDGLYPSNYEYKYPKAGEENSKVDVYVYQFKSGLTVKMDIPGREDDYIPRI
                     KWTNTENTLAIVHLNRLQNELTIIFGDALSGNTEDVLTYTEKKYIEINDDLTFLEDGQ
                     RFLFSHESDGYRHLYLYSNNGKLINQVTKGNWEMTTLYGCDNNAGKVYYQSCESSPIN
                     RSIYSVNLDGSGKKLLSAATGTNEAEFSSSFAYFINTYSDANTPYIVTLNNSEGELIR
                     EIENNNALRQDIKDKYCFSEKKFFTFTTSEGVELNGWMIKPANFKASKKYPVLFYVYG
                     GPGIQTVTNDWDYNLAWWQMLAQQGYIVVSVDNRGTGARGKDFRQVTYGQLGKYEVID
                     QIEAAKYLGAQKYVDKSRIGIFGWSYGGYMTALCMTIGADYFKAGIAVAPVTNWRYYD
                     SVYTERYNGLPQNNASGYDDNSPINHTSELKGSFLL"
     gene            complement(2201..2614)
                     /locus_tag="PLK75_10475"
     CDS             complement(2201..2614)
                     /locus_tag="PLK75_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39712.1"
                     /translation="MKRSKFWFSVKHYTDTIMFLSGFMALIFLSSCDCHINDFRNPYI
                     GKYQVHVAINSYGTCWEPYSNEYDTIISVTYGESDSSLLLMGREIYLDADGYFYEYHY
                     GCRLWSDSVWSSYMNGGLGCGQYETYLGHKISNKL"
     gene            complement(2705..4081)
                     /locus_tag="PLK75_10480"
     CDS             complement(2705..4081)
                     /locus_tag="PLK75_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOY39713.1"
                     /translation="MSKSPGNKPNAHELGTERPGKLLIRYSLPAIIAMAAMSLYNIID
                     RIFIGQGIGAYAISGLSLTLPLMNITTAFGAMVGVGASTLVSIRLGQHDRKGATQVLG
                     NAVSLNLILGILVTVVTLIFLDKILIILGASNETLPYAKEFMRIILYGNVITHLFFGL
                     NGVMRSSGYPRKAMYTTLITVAANIILAPLFIFVFHWGIQGAAWATVLSQLVGTAVLI
                     VHFSQIKSFLHFLPGYMKLKFSIVKEVISIGMSNFIMLISASVVISIINISLYKYSGD
                     FAIGAFGIINSISGLIFMIVIGFNQGMQPIVGYNFGAKKIPRLLRTFQLTVFAATIVT
                     CAGFLLAEIIPHTVSSAFTTDSQLTELSTEGLRLCMLMFPIVGFQVVTSSFFQSIGKA
                     KFSIFLALTRQVLFFIPILLILPHFWGLKGVWLSIPVSDFLSSLLSFIVLRWQVKKLK
                     AEHLTTGN"
     gene            complement(4458..5066)
                     /locus_tag="PLK75_10485"
     CDS             complement(4458..5066)
                     /locus_tag="PLK75_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39714.1"
                     /translation="MKQVKYINKIFIIGGILSIGLFANCNFNNDSNDLSSSDYFYFGN
                     NISDSKKLIICKLHPDYDTSKYLCTYDCIIKTSFKKEPIYVRLGIKGYNLYLLIPLLN
                     NVTEIPILNFENLDKEFWITQRNCSLIDPALLMESKLMNSEIIGNDTIYFIKNTLRYE
                     ELEFEENHIPHEENRIIVFSRKNGVNGFYTNETGENDFCWPY"
     gene            complement(5253..5858)
                     /locus_tag="PLK75_10490"
     CDS             complement(5253..5858)
                     /locus_tag="PLK75_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39715.1"
                     /translation="MKSLNISESYTSNSNGEEKKMIRKSYFVLVLMTLFFSKCNYITK
                     SNNNISSHKINTNINALSQNNTKENILNFCNALINDFKNNDTLNLINKTVFPLQVECF
                     SDKSKGILVEMKSFSIDFDNIFGDNFLIGLIYFKDKLNSSQQIDDYEVGVDLRDSTSF
                     TVSIQYKNLDNVGEMTDEYSRLFHFKMLDGDYKLVCVSCAG"
     gene            complement(6053..6487)
                     /locus_tag="PLK75_10495"
     CDS             complement(6053..6487)
                     /locus_tag="PLK75_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39716.1"
                     /translation="MLKKQELFSSAIIISILFFACSSSKKNYVGFVSNEFFVGMDLGE
                     FKNKAYGFYDYELSDSKQIVYTEIKDVKSDTIYTITESYYFNAENKKLISVKFLIVLN
                     EFLVNNNNYKEVLDNYFKNKIYTNKVKIEYLEVHYPTIVLTW"
     gene            complement(6459..7571)
                     /locus_tag="PLK75_10500"
     CDS             complement(6459..7571)
                     /locus_tag="PLK75_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOY39717.1"
                     /translation="MDDRDCFAIIDTLTVENGTTLTTPVETVRYQYSNHLGSACLELD
                     ASAAIISYEEYHPFGTTSYRSGRNEVDVSLKRYKYVGKERDEETGLYYYGARYYAAWI
                     ARFISVDPLNEKYPELSAYQYASNRPIIAIDLDGLEAKLLITDNIIGYTSQHIYGDQL
                     GVTEITVPVYDVILIDAQNPSKEIAHFGVTRDAWYSRGTNSEGTHLVNRAFEPKDGNK
                     NLYGTVPLNYPSGFSAYALRRQGNANLEAEPFTKDMNTYLNGEPIDDARENLGIANGV
                     MFHIGGYYNNGTKNKLAGSYGCFGFIPKNQIYKTKSFAEMVTRAKMYQSNKTSNESYN
                     DAINQIIEVKNNTNGIKTVQIQVEKRNNVEKARVVQ"
     gene            complement(7571..7813)
                     /locus_tag="PLK75_10505"
     CDS             complement(7571..7813)
                     /locus_tag="PLK75_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39718.1"
                     /translation="MKFGYIRVLSEWLGWRGKKRKDFGVTELKLCNFEKLTIRLCTAT
                     QLYRPCPILEAIPAAVTKFTANTSATASIKNGKPFT"
     gene            complement(8451..8561)
                     /locus_tag="PLK75_10510"
                     /pseudo
     CDS             complement(8451..8561)
                     /locus_tag="PLK75_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(8801..8874)
                     /locus_tag="PLK75_10515"
     tRNA            complement(8801..8874)
                     /locus_tag="PLK75_10515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8838..8840),aa:Arg,seq:tct)
     gene            complement(9136..>9480)
                     /locus_tag="PLK75_10520"
     CDS             complement(9136..>9480)
                     /locus_tag="PLK75_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39719.1"
                     /translation="MGYNSWEIEEGSATIKLFYNRETRFIVADAYLCNLPKENIMPVY
                     EYMLRENYTNDGLIFSVNNQAVLLSFISYEDDLSVDSGVELLHNLMQKADYYDDILIE
                     QFRGQPRVNEED"
BASE COUNT         2732 a         1889 c         1754 g         3105 t
ORIGIN      
        1 agcagaaatg agcccttcag ttccgaagta tgattgatgg gggagttgtc gtcgtagccc
       61 gaggcattgt tctgcggcaa accgttatat ctttcggtgt acaccgaatc gtaataccgc
      121 cagttggtaa ccggagcaac ggcaataccc gctttaaaat aatcagcgcc aatggtcatg
      181 cacagtgcag tcatataccc accatagctc cagccaaaaa tcccaatcct gcttttatcg
      241 acatacttct gggctccaag gtatttcgct gcttcaatct ggtcgatcac ctcgtacttt
      301 ccgagctgac cgtaggtaac ctggcggaaa tctttaccac gcgcaccggt tccgcggtta
      361 tcaaccgaaa caactatgta tccctgttgg gcaagcatct gccaccaggc aaggttataa
      421 tcccagtcgt tggttactgt ctgaatgccg gggccaccat acacataaaa aagcaccgga
      481 tatttcttgc tggctttaaa attggcgggt ttaatcatcc acccattcag ctcaactcct
      541 tccgaagtgg taaatgtgaa aaatttcttt tcggagaaac agtatttatc ttttatatcc
      601 tgtcgtaagg cattgttgtt ctctatttcg cgtatcagct ctccctccga attgttcaat
      661 gttactatat aaggggtgtt ggcatcgctg taggtattga tgaaatatgc aaaactggag
      721 ctgaattcag cttcgttggt accggtagca gcgctgagta gctttttacc gctgccgtcg
      781 aggttgaccg aatagatgct gcggttgatg ggtgaagatt cacacgattg ataatacact
      841 ttgccggcat tgttgtcgca gccgtacagt gtcgtcattt cccagttccc tttggttacc
      901 tgatttatca gttttccgtt gttgctgtaa aggtacaggt ggcggtagcc gtccgattca
      961 tgcgagaaca ggaagcgctg cccgtcttcg agaaaagtaa gatcatcgtt tatttcgatg
     1021 tatttttttt cggtataagt cagtacatct tcggtgtttc cgctgagtgc atcgccgaaa
     1081 attattgtta gctcattttg caggcggttc agatgtacga tcgccagtgt gttttcggta
     1141 tttgtccatt tgatgcgtgg aatgtagtcg tcctctctgc cggggatgtc cattttcact
     1201 gtgagccccg atttaaactg gtaaacatac acatcaacct ttgagttttc ttcaccggct
     1261 ttcggatatt tatactcata attgctggga tacaatccat cgaatagctg aatggaatat
     1321 tgttttacat tcgattcgtc ggttttaaga aatgccagat aatttccgtc gggcgaccat
     1381 tcgtatgcct tgctgaacga aaattcttct tcgtacaccc agtcgggagc gccgttaatg
     1441 atgtggttcc attcgccgtc ggtggtgatg ctttgttcaa tgccggttgc cagatcaaca
     1501 acgtaaatgt tgttgtttct cacaaaagat attttgctgc catcgggcga gaaacatgca
     1561 agttgctggg aaccttcttc cgaaaggggc ttcagtgttt tttctttcaa gtcgtaaatg
     1621 tagtaatctg ctttgaatga atgccggtaa atcgggtcgt aattggtgct gatcagcatc
     1681 atagtttcat cgtgattgaa ctggtaatcg aaaatccagg gaattttcac atctttaaag
     1741 tctttggctt taaacagatt ctcaaccatc tctcctgttt tgtacgaata tttgctgatg
     1801 gtattgcgac ccatattcag ggtggtgtaa tgttctccgt cgttcatcga cacaatgcca
     1861 tccacggtgt ttgggtagaa tgtaccggca ccgtaaatgt cttttaaggt gatttgggtt
     1921 tgtgaaaaaa ctcctaaaat gctgaagcat aacgcaatga gtaataatgt ccgtttcatt
     1981 ttgtttcgtt ttgatttcaa atataacaga tttctagtta acatttgtta aaatagattt
     2041 aaaccttgca ccggattgct gtgtaattaa tttttttcac ccttggttaa tcgctttgtc
     2101 accctgagtt aatcgaaggg tgatgtccga agtcgcattc cagcattccc ctgtctatgc
     2161 ttcgattaac tcagcatgac acccgaactc cgaaccctgc tcagagttta ttcgagattt
     2221 tgtgccccag ataggtttca tattgcccgc aaccgagtcc tccgttcata tagctcgacc
     2281 acacactgtc gctccagagc ctgcagccgt aatgatattc atagaaataa ccgtcagcat
     2341 caaggtatat ttcccttccc atcagcagca ggctggagtc ggattcacca taggtaacgc
     2401 tgattatggt gtcgtattca ttggaatagg gctcccagca tgttccataa ctgtttattg
     2461 ctacatgcac ctgatactta ccaatgtatg ggttgcggaa atcattgatg tggcaatcac
     2521 atgagcttag aaaaatcagt gccataaatc ctgaaagaaa cattattgta tcggtataat
     2581 gcttcactga aaaccaaaat ttgctgcgtt tcatactgtc ttgttaaaat gcaatatacg
     2641 cataagaatc aatcaaatct acaaaattga ttcgtagttt ctgaaaactt gaagtattat
     2701 cttatcaatt tccggttgtg aggtgttctg cttttagttt ttttacctgc cagcgcagca
     2761 cgataaaact caacagcgaa gaaaggaaat cagaaacggg aatgctaagc caaacacctt
     2821 ttagtcccca gaaatggggt aaaatcagca gaatgggtat gaaaaacaag acctgtcgtg
     2881 tgagcgcaag aaaaatcgag aattttgcct tgccaatcga ttggaaaaaa ctggaagtta
     2941 ccacctgaaa tccaacgata ggaaacatca gcatgcacag ccgcaagcct tcggttgata
     3001 attcggtcag ttgactgtcg gttgtaaatg ccgatgaaac cgtatgggga atgatttcgg
     3061 ctaataggaa cccggcacat gtaactatgg tagcggcaaa aaccgtaagc tgaaaagttc
     3121 tgagtaaacg cggaattttc tttgcaccga aattatagcc cacaatgggc tgcattccct
     3181 gattgaaccc aataacaatc atgaaaatga gcccggaaat gctgttgatg atgccgaatg
     3241 ccccgatcgc aaaatcgccg ctgtatttgt acaggctgat gttgattatc gaaatcacca
     3301 cgctggcgct gataagcatg ataaaattag acatcccgat tgaaatgacc tcttttacaa
     3361 tagagaactt tagtttcata tacccgggca ggaagtgcaa aaaacttttg atctgcgaaa
     3421 agtgaactat cagcacagcc gttcccacca gctgactcaa tacggttgcc catgccgcac
     3481 cctgaatacc ccagtggaaa acgaaaataa acaggggagc aagaataata ttcgctgcta
     3541 ctgtgatgag agtagtgtac atggctttcc gcggatatcc cgatgaacgc attacgccgt
     3601 tcaaaccgaa gaacaaatgg gtaatcacgt tcccgtacag gattattctc atgaactctt
     3661 tggcataggg tagtgtttcg ttgcttgctc cgaggataat gagtatttta tcaagaaaga
     3721 tcagcgtaac cactgtaaca agaataccca aaatcagatt caggcttact gcattgccga
     3781 gtacctgtgt ggctcctttg cggtcgtgct gcccgaggcg tattgacaca agggtgcttg
     3841 cacctacgcc caccatggct ccaaatgcgg tggtaatatt catgagtggt aacgtaagcg
     3901 acagtcccga aatggcatac gccccgatac cctgcccgat aaaaatccgg tcgataatgt
     3961 tgtaaagcga catggctgcc atggcaatga tggctggcag ggagtatcgg ataagcagtt
     4021 ttcccggtct ttccgttccc aactcatgag cgttcggttt atttcccggg cttttcgaca
     4081 tgaatgataa taattacagc ctacaaaaat aggatatatt tcaatcagcg ataaggtaat
     4141 gtaagccaat taacatttgc tgcaataata tttgttcgcc aggcaggtaa cctgttccgg
     4201 cttgtcgaag tacagattgc ttcatgttcg tacctcacat tcgcaataga cgggctctgt
     4261 tttcctctcc atccgaatca ctcagatagg actcggatat tttcatgctt cccaaagttt
     4321 ggggataatt cttattcgaa atatacccaa aatattttaa agatttctgt taacgtaagc
     4381 gctgaatatc aaggtaatac aatattgagt ttttgaaaag tgccatatgg tacacgaccg
     4441 tacacgaccc ctattaatta atatggccaa caaaaatcat tttcacccgt ttcatttgtg
     4501 taaaacccat ttacaccatt tttccgcgaa aatactataa ttctattttc ttcatgtgga
     4561 atgtgatttt cttcgaattc aagttcttca tatctgagtg tatttttgat gaaataaatg
     4621 gtgtcatttc ctatgatttc actattcatc aatttgcttt ccattaataa agcagggtca
     4681 attaatgaac aattcctttg tgttatccaa aattccttgt caagattttc aaaattcaaa
     4741 attggaattt cagttacatt atttaacaat ggtattaata aatacaaatt atacccttta
     4801 attcctaatc taacatatat tggctctttc ttgaaagatg ttttgattat gcaatcataa
     4861 gtgcataagt atttcgatgt gtcgtaatca ggatgtaatt tgcatattat tagtttttta
     4921 ctatcagata tgttattccc gaaatagaaa taatctgaac tgctcaggtc attgctatcg
     4981 ttgttgaaat tacaattggc aaataagcct atcgaaagta ttcccccaat tataaaaatt
     5041 ttattaatat attttacctg cttcatatag taaattttta gctatttgat caagtgattt
     5101 aatagatttt tcaatctcat tttttataat tcctgccgga ctatcttctg gaatgtcgtc
     5161 ataatcaatc gaatatcctt taagaaaact attttcctcc ccatcccagt cactctcaga
     5221 ggactcggat ataattggga atgacactac tgctacccgg cacaagaaac acaaaccaat
     5281 ttataatcac catctaacat tttgaaatga aataatcttg aatattcgtc cgtcatttca
     5341 cctacattat ccaaattctt gtattgtatt gagacagtaa acgatgttga atcacgcaaa
     5401 tcaaccccca cttcataatc atctatttgt tgcgaagaat ttaatttatc cttaaaatat
     5461 attaacccaa tcaaaaaatt gtccccgaaa atattgtcaa agtcaatact aaaacttttc
     5521 atctctacta atatcccttt acttttatca gagaaacatt ctacctgtaa tggaaaaaca
     5581 gttttattaa tcaaattcaa tgtatcatta tttttgaaat catttataag cgcattacaa
     5641 aaattaagta tgttctcttt cgtattgttt tgcgataagg cattgatgtt tgtattaatt
     5701 ttatgggagg atatgttatt atttgatttt gtgatgtaat tacatttact aaagaataac
     5761 gtcattaata ctaaaacaaa atatgatttt cttatcatct ttttttcctc cccatttgag
     5821 ttactcgtat aggactctga tatgttcagg cttttcaaat tttaaagata gttcttattc
     5881 gaaatatacc caaaatattt tattggaatt tgttaacata tatgcagatt aacaacgagt
     5941 tgcaaatttg tgtttaaaat aagtgccata tggtacagta ccacagatta tcaacgagtt
     6001 gcaaatttgt gtttaaaaaa agtgccatat ggtacagtac cgacactatc tactaccacg
     6061 ttaaaactat tgttgggtaa tgaacttcta aatattcaat tttaacttta tttgtataaa
     6121 ttttattttt aaaataattg tctaatactt ccttataatt gttattattg actaaaaatt
     6181 catttaaaac aattaagaat ttaacactaa ttagtttttt attttcagca ttaaaatagt
     6241 aagattcggt aatagtataa atggtatctg atttaacgtc ttttatttca gtataaacga
     6301 tttgttttga atcggataat tcataatcat aaaatccgta cgctttattt ttaaattctc
     6361 ctaaatccat gcctacaaaa aattcatttg aaacaaatcc cacatagttt tttttactac
     6421 ttgagcaggc aaaaaataaa atgctaatta ttatagcact actgaacaac tcttgctttt
     6481 tcaacattat ttcttttttc tacttgtatt tgtactgttt ttatcccatt tgtattgttt
     6541 ttcacttcta tgatttggtt tattgcatca ttataagatt catttgaagt tttattcgat
     6601 tgatacattt tggcgcgtgt aaccatttct gcaaaagatt tagttttgta tatttggttc
     6661 ttaggaataa acccaaagca tccatatgaa cctgctagtt tatttttagt gccgttatta
     6721 taataaccac ctatatggaa cattacccca tttgctattc ctaagttttc tctagcatca
     6781 tcaattggtt ccccattcaa ataagtattc atatcttttg taaatggctc tgcttcaaga
     6841 tttgcatttc cttgtcttct taaagcataa gccgaaaacc cagaagggta gtttaatgga
     6901 actgttccat ataaattttt atttccatct tttggttcaa atgccctatt aactaaatgt
     6961 gttccttctg aatttgtacc tctagaatac caagcatctc tagttactcc aaaatgagca
     7021 atttctttac tcggattctg ggcatcaatc aagattacat cataaacagg cacagtaatt
     7081 tctgtaacac ctaattgatc cccataaata tgttgagagg tatatccaat tatattatct
     7141 gttattaata attttgcctc caacccgtcc aaatcaattg ctattattgg tctattactc
     7201 gcatactggt atgccgaaag ttccgggtac ttctcattta gagggtccac cgaaataaac
     7261 cttgctatcc acgccgcata ataccttgct ccatagtagt aaagaccagt ttcttcgtcg
     7321 cgttctttac caacgtattt ataccttttc agcgatacat caacttcgtt tcttccactg
     7381 cggtaacttg tcgttccaaa cggatggtat tcctcgtagc ttattattgc agcgcttgcg
     7441 tcaagctcca aacaagcaga accaaggtgg ttagaatact gataacgtac tgtttcaacc
     7501 ggagttgtta atgttgttcc attttcgaca gttagagtgt caattattgc aaaacagtca
     7561 cggtcatcca ttatgtgaac ggtttcccgt tctttatcga ggctgttgct gatgtatttg
     7621 cggtaaactt cgtaaccgcc gccggaatag cttccagaat agggcagggg cgatatagtt
     7681 gggttgcggt gcaaagcctg attgttagtt tttcaaaatt acacaatttt aattctgtta
     7741 cgccgaaatc tttcctcttt ttccctctcc atccgagcca ctcggagagg actcggatgt
     7801 aaccgaattt catttccatt taagtagcac cgagtcccgt tccggtgact cggtgcgaga
     7861 ggtgcgtagt ttcctattta aaaagtgttt taatagaaaa tcttgttagt gtaacaacct
     7921 tctctccatc cgagtcactc gaagaggact ctgatgtaac cgaattccac taagtagcac
     7981 caagtcccgt gctggtgact tggagcgggg gaagaggagg ggaaattggt atctttgccg
     8041 catggtatat tatccagcgt ttgccagaat caatattgct gatcataatt aactgacctg
     8101 ctaattctac catgtattaa ataggttata aaatccaata tatacaagac aaccaagaaa
     8161 ccccattaaa actactataa ttttagttga tcttaatttg cttataaata cccctattaa
     8221 tgtattaatt atcagaataa ttataaatac taaagtataa ctatggtaaa actgttttac
     8281 ttgaaaggct gcttcacccg aagttttatt aaaaatgaaa taaaggacaa ttataatatg
     8341 aaataatatc aataaaccat ttataaaaat tggagtcctc ttcatttaat actcatttaa
     8401 tagtctacag atggcacacc atcaaaattt tcttttaacc cgttgtgcat ttaggtcaaa
     8461 tttagtttaa ttttattaat ggcacggata aaaaggaatt tattgaggaa ttgaatatta
     8521 gttaaggagg gaatttttgc aaggattcgg gtttcccctt ccatccgaat cactcggaga
     8581 ggattcggag tgggaggcga gtagtttcca aatttataaa gtgccatatg caatagcgca
     8641 gatacgcaca attcgttacc actttttgaa attatttttg gtaacgattt ggtaacgaaa
     8701 gcgggttata tattgcgtta ttttgcgctt ttgatagtaa acgcaaaaac ctgaaagcat
     8761 tgaatttcag gtttttgcgt tgttttgctg gttgcgactg tagtccggac aggaatcgaa
     8821 cctgcgacct acggcttaga aggcagttgc tctatccaac tgagctaccg gaccaagatt
     8881 ttgcgatgca aagatattaa aaattgcttt ttaaaaactg cttgtcgaaa ttttattgat
     8941 tagttgattg gttgattagt taatttgctg attggcggat tgtttgttag tttatttttg
     9001 atttgcatgc tttttcttca tagtgcccat aatactgttg agtagttttt taatactttg
     9061 caattgcttc aacaactcat ccggattttt aaaagcggtt gatttctcac atcctcacat
     9121 ccatcaatca tcacatcaat cctcttcatt aaccctgggt tgtcctctga attgttcaat
     9181 aagaatgtcg tcgtaatagt cggctttctg catcaggttg tgcagaagtt caacgccgga
     9241 atcaacgctc aggtcgtctt cgtacgaaat aaacgaaagc agtactgctt gattgtttac
     9301 gctgaatatc agtccgtcgt tggtatagtt ttcgcgcagc atatattcat acaccggcat
     9361 aatgttttct ttcggcaggt tgcacaaata ggcatcagcc acaataaagc gggtttcgcg
     9421 gttgtagaac agtttaattg tcgcgctgcc ctcttcaatt tcccagctgt tgtagcccat
//
LOCUS       DAOOQP010000114         9436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9024_length_9436_cov_4.774118, whole genome shotgun sequence.
ACCESSION   DAOOQP010000114 DAOOQP010000000
VERSION     DAOOQP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9024_length_9436_cov_4.774118"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLK75_10525"
     CDS             complement(<1..527)
                     /locus_tag="PLK75_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOY39720.1"
                     /translation="MEIPFSESYKIKMVEPLKRSTYDERLQWIQEAKYNLFKLRGDQV
                     FIDLLTDSGTGAMSDRQWAAIMTGDESYAGARSYFEFRDAVTSIMGFEHVLPTHQGRA
                     AENVLFSVLVKPGDIVPGNSHFDTTKGHIEFRKAHAVDCTIDEAFDTTIEHPFKGNID
                     LNKLEAVLKAHPKEK"
     gene            complement(775..3639)
                     /locus_tag="PLK75_10530"
     CDS             complement(775..3639)
                     /locus_tag="PLK75_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOY39721.1"
                     /translation="MNPFLKQVSDYLKLQYPGEMEQVCIVFPNMRPALYFRRYFAQGN
                     SQPVWTPEICTITQLFENASSLSAKDELSLMFDLYRTYIKLTSSAETFDNFYSWARVL
                     LTDFDDVDTHLADAQKVFSNVKDLKEIDLLIGNDVYIDKDIVQAFWGNILSSRKSAER
                     DAFLQTWMILPELYHDHRSGLKNENQGSQGMIYREVAENPERIDTTKTYLIVGFNVLN
                     ECEKRIFSFLSKEGKAVFLWDYDEYYMNNPSHEAGYFMRMNVKQFPMPPDFSLHNKGI
                     RHNPPEITCIASPSAVAQVKYACWLVSEKIREGAFVAEETAIILGNEDLMPLVLQSLS
                     SEIGPYNVSMGVSIKDSNAVAWIRNIMLLQIKIRHAGVPRFYFKYVNALLNHPFTRLL
                     SPHTSEMVSALRTQKIIYVSPEFFSGKNFLEELFQYSGGNVHDFSEYLLRMLSKVLTA
                     EKQEDGFEVQREIVYQIYRSLKYIDRIIAEKEISFSRLATYSHLVDQIIKTTVVPFEG
                     EPLQGLQVMGLLETRLLDFKNILILSVNEGVLPKNTLAPSFIPVNLRYAFHLPSQQHT
                     DSLYSYYFYRLLQRSSSVGLIYKMATKDVGGGERSRFIQQLKFELPNSVNFKQINYSV
                     VAKANSLITIPKNKNIAAFLNAFAEGKIAHKKLSASLLNQYIKCPLAFYFSTVAGISE
                     PESVEEDTNMKQFGNIFHKAIELIYKEFALSGKTINASDLASIADIHNNDLVEKSLLQ
                     AFDIEYKTSFLLKSELQGKQRLIYEVLLKYIGRLLHYDMEKAPFRIASLEKQLLFSMP
                     FIDGNNVRTALIKAKIDRVDVQNDSTVVLDYKTGKVERKFKNIAALFEPGRGKEMDYF
                     FQILLYCVMLKNAEKIIGKVRPGLISLRNLKDDSNCTIHIKNDTADYDILNIEGELEN
                     EFSVALQDLIAEIVNPRIPFVQYPEKTKCSYCIYNTICSV"
     gene            complement(3636..4619)
                     /locus_tag="PLK75_10535"
     CDS             complement(3636..4619)
                     /locus_tag="PLK75_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOY39722.1"
                     /translation="MNSFGNLFRITLYGASHESEVGVIIDGCLPGLALSVNDFTEIIN
                     RRKPTATGGTLRNESDTPEFRSGIYQGFTTGAPLNISFRNRDVKSEDYNFDGFYRPGH
                     ADYTASVKYQGYSDPRGGGIFSGRMTLPLVAAGVVALKMLQGISVSAELVKAGGSTEI
                     HAAVQNASQHGDSVGGVVKCEICGVPAGWGEPFFDSVESLLSHLLFSVPGIRAISFGD
                     GVESADMLGSEFNDVFIGENGETATNHSGGINGGITNGNNLIFSVYCKPTSSITKGQQ
                     TFNFTAKEMSHFSVPGRHDVCFALRIPVIIEAAAAIVLADLKLLHFNNCCR"
     gene            complement(4612..5868)
                     /gene="aroA"
                     /locus_tag="PLK75_10540"
     CDS             complement(4612..5868)
                     /gene="aroA"
                     /locus_tag="PLK75_10540"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOY39723.1"
                     /translation="MITILPSEISGIAKLPPSKSHAQRLLVAAMLANGISEIHNAGQA
                     EDVKAVASIVQRCARSLSLDNDRYTVKGGIDFLPSSVFDCGESALCARLMVTVLSTKM
                     KPFTITGRGSLLKRPVAFEMHALQSMGLKTENKGKYLPFTVLGGKMQTGSYQLDTSVS
                     SQFASGMMMTLPTLKGFSTLELKNLKSRPYVLLTLDVMKLFGIECEFSTDNIFTCHGD
                     QHYKAASVTVEPDWSSAAALMVAAALCGEIRLEGLNMHSEQADIRVLDLLSEAGVSYT
                     ADDNHIQILKSKPHAFKFDFTNCPDLFPVASVLAAGANGVSLLQGLKRLKFKESDRHG
                     LVYRQLQRAGIPVLVREDEIEITGKPVEKFFTTDSHCDHRMAMAFSVLALQSQKGAKI
                     KNHECVNKSFPGFYETLKQLGGKIDE"
     gene            complement(5865..6893)
                     /gene="aroB"
                     /locus_tag="PLK75_10545"
     CDS             complement(5865..6893)
                     /gene="aroB"
                     /locus_tag="PLK75_10545"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOY39724.1"
                     /translation="MQSDYEEIIIHTKKTYPVLICDACKEVESRIPSNSIIITDSNLM
                     HYYHNALEKYQCIVIPAGEESKQWKSLEIIFGELVRMNAGRDTFLLGFGGGVVLDITG
                     FVASTYKRGCRFGFVATSLLAQVDASVGGKNGINFGGYKNLVGVFNQPEFVVCDGSLL
                     ATLPRIDFINGMAEVIKHALIADSNFYDYLVMQAPLILQLDKYSINHIVESSVQIKSG
                     YVIGDEYDQGLRHHLNFGHTFGHAFELSENIGHGYAVAKGMMVAAEISFRMKLCNRQL
                     VEKTKQILLVYNLPVDFTINQEVMNSVMNDKKNRSGKISMVLIKGIGETIMVDFELDE
                     LKKIAEQL"
     gene            7035..7952
                     /locus_tag="PLK75_10550"
     CDS             7035..7952
                     /locus_tag="PLK75_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY39725.1"
                     /translation="MAIPTSRTKVNAEIRIDIDRCSGCGLCTQVCTDSNIVIANAKAK
                     TTAGSVFGCIACGHCMAVCPENAISINGRTMSFSDLIPLSEKHKMANYDELLALMQSR
                     RSIREFRDKNVDDDTIDKILEYASFSPMGLPPSDVHVIVFRGKDKVNAFAKDYSDFLK
                     GMKYITSNWFITLMRPFWGKANDEMFRNFIKPAVNTFTKQMDCGRNMITYDAPLMFYF
                     YGSPYIDPADPIIAATYAMHAGVSLGLGTCMLGAIHPFLQYGKNGKLFREKYGIKYKS
                     REGLFLIMGYPKVHYHNGLKRSFAEVKKL"
     gene            complement(8005..8130)
                     /locus_tag="PLK75_10555"
     CDS             complement(8005..8130)
                     /locus_tag="PLK75_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39726.1"
                     /translation="METQIQENEPKNKKWYQGGTVPFIIMLVVLVGAMVVLGIVL"
     gene            complement(8240..8578)
                     /locus_tag="PLK75_10560"
     CDS             complement(8240..8578)
                     /locus_tag="PLK75_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39727.1"
                     /translation="MKSLLKMMIALVLIPSILVLICNANNTATSQRNVGGNPAEKVFT
                     LPGTPIAVVSKEESNHCPVYDKFRVIFSLSLRIINNNFHFYSASESMHIVSVCHDKMP
                     KYLQTMCFKN"
     gene            complement(8609..9385)
                     /locus_tag="PLK75_10565"
     CDS             complement(8609..9385)
                     /locus_tag="PLK75_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOY39728.1"
                     /translation="MDLTFDNTMIVSEIKILKSHGIQYVPDLKNATLTELFRGKPEID
                     NSLTDSEKETLVKLCPTDAFSKDEFGIDLGKCVFCKECSFACGGKIRFTNDYKIAANQ
                     RSNLIVTQGRESEFFSETAIRPEIRKLFRNSLKLRQVSAAGDNSCEMELNASGNVNFD
                     MGRYGIEFVASPRHADGIVITGPISQNMAEALQITYDAIPSPKIIILVGSDAIGGGVF
                     EGSKALKRDFIDKYPIDLFVPGNPAHPLTFINGVLKLLGK"
BASE COUNT         2739 a         2071 c         1808 g         2818 t
ORIGIN      
        1 attttttctt tgggatgggc tttcagcaca gcctcaagct tattcagatc gatattgcct
       61 ttaaaggggt gttcgatagt ggtatcgaaa gcttcgtcga tggtgcagtc aactgcatgc
      121 gccttgcgaa actctatgtg ccctttggta gtatcgaaat gagaattacc gggtacaata
      181 tctccgggct taacaagcac actaaaaagc acattctcgg cagccctgcc ctgatgggta
      241 gggagcacat gttcgaaccc cataatgcta gtaacagcat cgcgaaactc gaaatacgaa
      301 cgtgcgcctg catagctttc atcaccggtc ataatagctg cccattgacg gtcgctcatt
      361 gcacctgtgc cactatcggt gagcaagtcg ataaaaacct gatcgcccct gagtttaaag
      421 agattgtatt tagcctcctg tatccattgc agccgttcgt cgtaagtact acgtttcagc
      481 ggctcaacca ttttaatttt atacgattcc gaaaaaggta tttccataac ctaaggattt
      541 aaaaataaat actaaattaa atacacgaaa tggttgatag tggttagaaa gcgtagctgt
      601 cgcgatcttt tatatgcctg aagatctgct tggtagttaa caatatggtg ttgtttttcg
      661 acataataat cactaatacg cttaacaaaa ttatattttt ttattttaaa acaaatccgc
      721 tttaataaaa taaatattaa caagttaaca ggcgtttatt ctcgttttta acccttatac
      781 cgagcaaatg gtattgtaga tacaatagct gcattttgtt ttttcggggt actgaacaaa
      841 gggtattcgc gggttaacaa tttctgcaat taggtcttgt aatgcaacgc taaattcatt
      901 ttccaattca ccctcgatgt ttaagatatc ataatcggca gtatcgttct ttatgtgtat
      961 tgtacaatta gaatcatcct tcaaattgcg taacgaaatt agtcctggcc gaactttacc
     1021 gataattttt tccgcatttt taagcattac acaatacaac aatatctgga agaaataatc
     1081 catttccttg ccgcgtccgg gttcaaacaa tgcagcgatg tttttgaact tccgttcaac
     1141 tttgcctgtt ttataatcaa gtacaacggt gctatcattc tgcacatcta ccctgtcgat
     1201 tttggctttt attaaggcag tgcgcacatt gtttccatct ataaaaggca tgctaaaaag
     1261 taactgcttt tcgagtgaag caatcctgaa aggcgctttt tccatatcgt aatgcagcaa
     1321 tcgcccaatg tatttcagca aaacttcgta aataagcctt tgctttccct gaagctctga
     1381 ttttagcaag aaagacgttt tatattcgat atcaaaggct tgaagcaacg acttctctac
     1441 cagatcgtta ttatgaatat cggcgataga tgccaggtcg ctagcattaa tagttttacc
     1501 actcaaagca aattccttat aaatcaactc tattgcttta tgaaagatat tgccaaactg
     1561 cttcatatta gtatcttctt caacactttc aggttctgaa atccccgcta ctgtactaaa
     1621 ataaaaagca agcggacatt taatgtactg attaagcaaa ctggctgata gttttttgtg
     1681 tgcaatcttc ccttcggcaa aagcattcag gaatgcagca atattcttat ttttaggaat
     1741 ggtaattaag gaatttgctt tagcgacaac actataattt atctgcttaa agttcaccga
     1801 atttggcaat tcaaatttta attgttgaat aaaacgggac cgttctcctc cgccaacatc
     1861 cttagtcgcc attttataaa tcagtcctac tgaagaagag cgctgcaaca gacgataaaa
     1921 atagtaggaa tacagcgaat cggtgtgctg ttgcgaaggc aaatgaaaag cataacgcag
     1981 gttaaccggt ataaacgaag gggctaaggt atttttgggt aacacacctt cgttcaccga
     2041 tagaatcaaa atatttttaa aatcgagaag ccgcgtttcg agtagaccca taacctgtaa
     2101 accttgcagt ggttctccct cgaatggaac aacagttgtt ttgattatct ggtccaccaa
     2161 atgcgaatag gtagcaagtc tggaaaaaga tatttccttt tcagcgataa tacggtcgat
     2221 gtatttaaga gacctataga tctggtacac tatttcgcgt tgcacttcga agccgtcttc
     2281 ctgtttttcg gctgtgagaa ctttagaaag catgcgcaac aaatactctg aaaaatcatg
     2341 cacattgcca cccgaatatt gaaaaagttc ttctaaaaag ttcttaccag aaaaaaattc
     2401 aggagagaca taaataattt tctgggtacg caaagcactc accatttcgc ttgtatgcgg
     2461 cgacagcaaa cgtgtaaacg gatgattcaa tagtgcattt acatatttaa aataaaacct
     2521 gggtactccc gcgtgcctta ttttaatctg cagaagcatg atgtttctaa tccaggctac
     2581 tgcatttgag tcttttatac taacccccat cgagacatta taaggtccga tttcagagct
     2641 caaactctgt aatactaagg gcatgaggtc ttcattaccg agtattattg ctgtttcttc
     2701 agcaacaaaa gcgccttctc tgattttttc ggagactaac caacaagcgt atttcacctg
     2761 tgctactgct gatggagatg caatgcaggt aatttcaggc gggttgtgtc gaattccttt
     2821 gttatgcaga ctgaaatcgg gaggcattgg gaattgtttc acattcatgc gcataaaata
     2881 gcctgcttca tggctggggt tgttcatgta gtattcatcg taatcccata ggaatacagc
     2941 ctttccttct ttacttagga agctaaatat tcttttttcg cattcattca gcacgttgaa
     3001 acccacaatt aaataggttt tggtggtatc gatacgttcg gggttttcgg caacttcgcg
     3061 atatatcata ccctgcgacc cctggttttc gtttttcagc ccgctgcggt gatcatgata
     3121 caactctggc aaaatcatcc atgtttgtaa aaaggcgtcg cgttctgccg atttccgtga
     3181 tgataatata ttaccccaga atgcctgcac aatatccttg tcgatataaa cgtcattgcc
     3241 aatcaaaagg tctatctctt tcaggtcttt tacattacta aacacttttt gggcatcagc
     3301 aaggtgtgtg tccacgtcat cgaaatcagt aagcaaaacc ctggcccagc tgtaaaaatt
     3361 atcgaatgtt tcggcagaag atgtaagctt gatgtaagtc cgatataaat cgaacatcaa
     3421 acttagctca tctttggcac ttaatgatga tgcattctcg aacaactgtg taatggtaca
     3481 tatttcagga gtccaaaccg gttgggaatt cccttgtgca aagtatcgcc tgaaatagag
     3541 ggcaggcctc atgttgggga atacaatgca tacctgctcc atctcaccgg gatattggag
     3601 tttcaggtaa tcgctaactt gtttcaggaa agggttcatc ggcagcagtt gttgaaatgc
     3661 aacaatttca ggtctgccaa aacaattgct gcagctgctt ctattatcac cggaattctg
     3721 agggcaaagc acacatcatg gcgaccggga acgctgaaat ggctcatttc ttttgcagta
     3781 aaattaaaag tttgttgccc cttagtaata cttgatgttg gcttacaata tacgctgaat
     3841 atcaaattat taccattagt tattccgcca ttaattcctc ctgaatgatt ggttgccgtt
     3901 tctccatttt ctccaataaa aacatcgtta aactccgatc ccagcatatc agcggactcc
     3961 acgccatcgc caaatgaaat ggctcgtatg ccgggaaccg aaaataacag gtggctcagc
     4021 aacgattcga cagaatcgaa gaaaggctct ccccaccctg ccggaacacc gcaaatctca
     4081 catttcacca caccgccaac agaatcacca tgctgagaag cgttttgcac agcagcatgt
     4141 atttcggtac tgccacccgc tttaaccaat tcagcactta ccgaaatccc ctgcaacatt
     4201 ttcaaggcaa ccacacctgc tgcaaccaat ggtaaagtca ttctaccgga gaagatacca
     4261 ccgccacgcg gatcgctata gccttgatat ttcacgctgg ctgtataatc ggcatgtccc
     4321 ggccggtaaa atccatcaaa attataatct tcgcttttta catccctatt tcgaaagcta
     4381 atattcaggg gtgctcctgt agtaaagccc tgataaatac cagacctaaa ttcaggcgta
     4441 tcgctttcat tgcgcaaagt accgcctgtt gctgtaggtt tacgacggtt aatgatttca
     4501 gtgaagtcgt ttactgaaag ggctaaaccg ggcagacaac catcgataat tacacccact
     4561 tccgactcat gtgatgcgcc gtatagtgtg attctgaaaa ggtttccgaa gctattcatc
     4621 gatttttcct cctaattgtt ttaatgtttc gtaaaacccg gggaatgatt tatttacaca
     4681 ttcatgattt ttaattttag cacctttctg cgattgcagg gcaagtaccg aaaatgccat
     4741 cgccatacgg tgatcgcaat gactgtcggt agtaaagaat ttttcgacag gttttcctgt
     4801 tatctctatt tcatcttcgc gcaccagcac gggtattcct gctctctgaa gctgacgata
     4861 aaccagacca tgcctgtcgc tttccttgaa tttcagcctt tttaaacctt gcagaaggct
     4921 aaccccattg gctcctgctg ccaacaccga tgccacaggg aacaggtcgg gacaattggt
     4981 gaaatcaaac ttaaaagcat gaggtttgga tttaagaatc tgaatgtgat tgtcatcggc
     5041 tgtatagctt actccggctt cactcagcaa atccaatacc cgtatgtcgg cttgctcaga
     5101 atgcatgttt aagccttcga ggcgtatttc gccacagaga gcagctgcaa ccataagggc
     5161 tgccgcgcta ctccagtccg gttccaccgt cacgctggca gctttataat gctgatcgcc
     5221 gtggcaggta aatatgttat ccgtcgaaaa ttcacactca ataccaaaca atttcattac
     5281 gtcgagtgta agcaatacat agggcctgct ttttaggttt ttcagctcca gtgtcgaaaa
     5341 ccctttcagt gtagggagag tcatcatcat tccactggca aactgcgacg aaaccgaagt
     5401 atcgagctgg tatgagccgg tttgcatttt acctccaagt accgtgaaag gaagatattt
     5461 tcccttattt tcagttttca gtcccatgct ctgcaatgca tgcatttcga aagctaccgg
     5521 acgtttcaga aggctaccgc gccccgtaat ggtaaaaggt ttcatcttcg tgctcagcac
     5581 tgttaccatc aagcgagcgc acagggctga ttccccgcaa tcgaaaaccg atgaaggaag
     5641 gaaatcgatc cctcccttta ccgtatatct atcattatcg aggcttaaac tccgtgcaca
     5701 gcgttgcact atcgatgcta cagctttcac gtcttcggct tgtccagcat tgtggatttc
     5761 cgatatacca tttgcaagca ttgcagccac taaaaggcgt tgtgcatggc tttttgaagg
     5821 cggtaattta gctattcctg aaatttcgga gggtagaatt gttatcataa ttgttcggct
     5881 atttttttca actcatcaag ttcaaaatca accattatgg tttctccaat ccctttaatc
     5941 aacaccatgc ttattttccc agaccggttt tttttgtcgt tcattacgct gttcatcact
     6001 tcttggttga tagtaaaatc aacaggcaaa ttataaacta aaagaatttg ttttgttttt
     6061 tcgaccaatt gccggttgca caatttcatc ctgaacgaaa tctcagcggc aaccatcatg
     6121 cctttagcaa cagcatagcc atgtccaata ttttcgctta gctcaaaagc atgaccgaat
     6181 gtatggccga aattcaaatg atgacgcaaa ccctgatcgt attcatcgcc aataacataa
     6241 cccgatttta tctgtaccga agactctact atatggttaa tagagtactt atcaagctgt
     6301 aaaatcaatg gtgcctgcat tacgaggtag tcataaaaat tgctgtcagc tatcaaggcg
     6361 tgtttaatca cttcagccat tccgttgata aaatcaattc ttggaagagt agctagcagg
     6421 ctgccgtcgc agaccacaaa ttccggctga ttgaaaacgc caaccaggtt tttataaccc
     6481 ccgaaattta taccgttttt cccccccaca cttgcatcga cctgagcaag gaggctggtt
     6541 gcaacaaacc caaaccggca gcctctttta tatgtagaag ctacaaaccc cgtaatgtcg
     6601 agaaccacac cacccccaaa gccgagaaga aacgtatcgc gtcctgcatt catacgtaca
     6661 agctcaccaa aaattatttc cagacttttc cattgcttcg actcttcgcc tgccggaatc
     6721 acaatacatt ggtatttttc tagagcattg tggtagtaat gcatcaggtt cgaatcggta
     6781 ataataatgc tgtttgaagg gatgcggctt tcaacttctt tacatgcatc gcatattaaa
     6841 acgggataag ttttttttgt gtgaataata atttcctcgt agtcactttg cattattatc
     6901 tgggacctgg tttattctgt aaaaataaaa catataattc aagatatgtt gctaatacca
     6961 ttttttttaa aatttagaat ttttaaaatg taaaattctc tgattttcaa ttaattttat
     7021 cgaaaatttt cggcatggct atacccacat cccgcacaaa agttaacgct gaaattcgca
     7081 tcgacattga tagatgtagc ggttgcggac tatgtacaca ggtatgtacc gacagcaata
     7141 ttgttattgc gaatgcaaag gcaaaaacca ctgccggctc cgttttcggg tgcatagcat
     7201 gtggtcattg catggcagtg tgtcctgaaa atgccatcag tattaatggc agaacaatga
     7261 gtttttctga tttaataccc ctttccgaaa aacataagat ggccaattac gatgaattgc
     7321 ttgccctgat gcaatcgcgc cgaagtatca gagaatttcg cgataaaaat gttgatgatg
     7381 atactattga taagattctt gaatacgctt ctttcagtcc gatgggtctg ccaccttccg
     7441 atgtgcacgt aattgttttc aggggcaaag ataaagttaa tgcctttgca aaagattaca
     7501 gcgactttct gaaaggcatg aagtacatta catcaaactg gtttattacg ctgatgcgac
     7561 cgttttgggg caaagctaac gacgaaatgt tcaggaactt catcaagcct gctgtaaaca
     7621 ctttcaccaa acagatggat tgtgggcgca atatgattac ttacgatgct cctctgatgt
     7681 tttatttcta tggctctcct tacatcgatc cggcagaccc aataatagcc gcaacctatg
     7741 caatgcatgc cggagtatcg ctaggactgg ggacttgtat gctcggagca attcaccctt
     7801 ttctgcagta tggaaaaaac gggaagcttt tccgtgaaaa atatggcata aaatataaaa
     7861 gcagagaagg tttgttcctt attatggggt accccaaagt gcactaccat aacgggttaa
     7921 aacgcagctt tgcagaggtt aaaaagcttt aaaaaaaagc cacccattgc agagtggctt
     7981 atatttctaa agaaatcgtg tttatcaaag cacgatgcca agaacaacca ttgctccaac
     8041 gagcacaaca agcatgataa taaaaggcac tgttcctccc tgataccatt ttttgttttt
     8101 aggttcattt tcctgaattt gtgtttccat gttaattatg atattctgat accaacatat
     8161 ataatctatg cagtatcggt taattactaa aaatcgaatc actattatct gccattggca
     8221 gtgcaaaaaa tagagaattc taatttttaa aacacatggt ttgcaggtat tttggcatct
     8281 tatcgtgaca gacgctcaca atatgcattg attcggaagc tgaataaaaa tggaagttat
     8341 tattgattat cctgagacta agcgagaata tcaccctgaa tttgtcataa accgggcaat
     8401 gatttgactc ttctttcgaa accacagcaa taggagttcc cggcaatgta aaaactttct
     8461 ctgcagggtt cccacccaca tttcgctgag acgttgcagt attgtttgca ttgcatataa
     8521 gaaccagtat tgaaggaatc aatactaaag caatcatcat tttaagcagc gatttcatat
     8581 caatattagc ctgaagcaaa attataaatt atttacccaa taatttcaaa acaccgttaa
     8641 taaatgttag tggatgagcc gggttacccg gaacaaacaa atcaatcggg tatttatcga
     8701 taaaatcacg ttttaatgct tttgaaccct caaaaacacc tcctccaata gcatcgctgc
     8761 caacaagaat aatgattttc ggtgagggaa tggcatcgta ggttatctga agcgcttctg
     8821 ccatattctg cgatatgggt ccggtaatta caataccatc ggcatgcctt ggagatgcaa
     8881 caaattcgat tccatagcgc cccatatcaa aattcacatt tcccgatgca ttcagctcca
     8941 tttcgcatga attatcgccg gcagccgaca cctgccttag tttcagtgag tttctgaata
     9001 actttcttat ttcgggacga atggcggtct ctgaaaaaaa ttctgactct ctgccctgcg
     9061 ttacgatcaa attgctgcgc tggttggctg caattttgta atcgttggta aacctgatct
     9121 ttccaccaca ggcaaaacta cattccttgc aaaagacaca cttgcccagg tcaattccaa
     9181 attcatcctt cgaaaaagca tctgtcgggc atagcttgac aagtgtttcc ttttcgctat
     9241 cggtgagaga attgtctatt tcaggcttgc cgcgaaacaa ttccgtaagt gtcgcatttt
     9301 tcaggtcggg aacatattgg attccgtgac ttttaagtat ttttatttcg ctgactatca
     9361 tagtattatc aaaggtcaaa tccacaataa cttaaattaa aacttttgtt gcaaaccgga
     9421 aaatcagata tctcct
//
LOCUS       DAOOQP010000115         9420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9045_length_9420_cov_5.110625, whole genome shotgun sequence.
ACCESSION   DAOOQP010000115 DAOOQP010000000
VERSION     DAOOQP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9045_length_9420_cov_5.110625"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="PLK75_10570"
     CDS             <1..1863
                     /locus_tag="PLK75_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39729.1"
                     /translation="AGTLELTPLTTTTYTVSVVDAHNCESNTVSMTITVSPHLEITEV
                     LLQQNRCFHSCDGSAELVIIGGIPPLNYSWGSTNHIYNNLCAGIYTVTVSDQIGCFAT
                     TYFLITEPDTLTYSINTVDASCYGSEDGTANIIVAGGTLPYSFLWPNNATTSFITTGA
                     GDFVVTVTDAHSCRIEASYTVGQPTELVVQPAGNPQICIGQEATVSAQAAGGTPYYDF
                     HWTGSDGTTYPTHLFHVSPDTTTIYTVTVVDSHGCLGNVATVTVNVYPPVEIESVTTS
                     YDTVCQGERAIIYVNVIGGNGGPYIINLENGATVGSPFTVFPGFTSTYHVTASDMCGS
                     PSASDSITITVMPFPEVSFTADHVNGCSPAVVHFTSLGNDENYSYLWDFGDYNFADIQ
                     NPMHIYNSAGTYDVSLIVTSPYGCQTSSVTNDLITVHPTPTASFYLEPEIATLLESEV
                     VFTNLSQDAMRYFWYFGDGDSSLFVHPRHQYADVGEFEVYLVAESEFGCRDTVMRRVY
                     VENIITFYAPETFTPNGDGINDCFRVCGNGIDPNHFALSVYDRWGECVFSTEFYDTEV
                     SCDACSSGSWDGTDNGNRLAGDKYLPMGLYTWYCEFKDVNNITYKKSGKIRLIR"
     gene            2058..2480
                     /locus_tag="PLK75_10575"
     CDS             2058..2480
                     /locus_tag="PLK75_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39730.1"
                     /translation="MKHFALFFLLLLSFAAYSQSDDEVIPDSRLYVRFSADYINVMLQ
                     SDPKSVEYLNFELDFGYKIMDAGLEKCEGLPYLHYFNSTDKTVEGIVEDINESSINIM
                     EYHYDRNYDKPNIYRIGNTGQVIIFYSLKDLNRKFNQN"
     gene            2482..7032
                     /locus_tag="PLK75_10580"
     CDS             2482..7032
                     /locus_tag="PLK75_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39731.1"
                     /translation="MKRISGIIIIILLLGILGNLKAQTYLISQGGTITTCSGTLYDSG
                     GANGEYGTYEDYTITICSSTGGSISLLFTVFQLESATYDHLSIYDGPNTSANDIIYHA
                     GGSTLLNQEITGSGNCLTLVWHTDGSVTYPGFAATISCTYPCQDYTIDIVSSNPPQSP
                     PADSLWVDVCQGETVTFTATADFSNNNVDYNQTLANTTWYWTVVSEAGTEDLGGLGQN
                     TLTYTFTNEGGYYINLQTVDMNGCTQILNDYWRVRASLTPDFTVTHPATVCPGETFDL
                     TGTASVDPWVLQIPEVAFIEVCFEDVVGVDQEQCFVHSAFAPGQFITAASDVESICMN
                     MEHSYMGDLDIWIECPNGQTANLFEQACGGTYFGEANDNDDCVPGVGWNYCWSMSGSS
                     LMSANCYTGSSLPAGTYLPVGSFSSLIGCPLNGEWCIHFLDNLSIDDGNVFTVELHFA
                     DYLIPSAENMWTYTTTYNTSPTSTDLEWTGEGVGANTGGVTTASSLTPGSQPFTFSAT
                     DNLGCTYDTTVYVTVLPFNDPTCCVFPDPNAGNDDQVCSNSYTFNGTITQGNTANWTL
                     VSGPGTGTWTNQTSPAATVTVSAYGTYVFQLYEQNLSPSCSATDQVTIVFLENPQSTF
                     TVTTIPCFGGNTTVTYTGNAQSTATYNWNFNGAQVISGSGAGPYVLSWTTAGAHPVSL
                     QVVQDGCTSTNTTNSVMMPAVLSHVLTTHNDPCYQSCQGNASVLVTGGTAPYTYSWAS
                     GNNYLPNLCAGNYAITVTDANGCTTVESFSISEPTQMVMTSYQTTNLSCYNAADGAIA
                     VVAQGGTGNLMYIWSDIGVGEPTRVGMSAGNYSLTIMDENNCQLVEFFQLTQPDELLL
                     TISNSLSICEWQTAHIVAQHMGGTAPYSFFWDTGTGYEIGPNELNTVPHQTTTYNVYV
                     QDGNGCKSNTASMTVTVSPELIIDTILLQHNRCFQSCDGRAELQITGGIPPLQYSWGS
                     ADNVLAGLCAGIYTVTVTDEIGCTINDVFTITQPDEITYTLNTVPASCYNYNDGEATI
                     YVQGGVLPYTYLWPDGDTDMTMLNHSGVYIVTVTDDHNCRIEVPITITQPDDILLISS
                     DDKQICIGQNAILSAQAVGGVPYYSFLWQGTDGSTFPTASITVNPDVTTEYSVTVTDS
                     HGCMGNVGLVRVNVYPPLTITDIVTSYDTVCPGEPAIIEVDAAGGNGGPYMLLLEDGS
                     VVPSPFTVYPQVTTMYYVTLMDACGSPSAVDSIKINVYPAPPNLFVSDRVSGCPGVAI
                     NFTELNSVNSLSYLWNFGDNGYSIEKNPAHIYTEPGVYSVSLTTRTSFGCSTTQTIPY
                     MITIFENPVASFTVTPEIVSVLDGDVFFENLSSLADSVYWFFGDGDSSIYYSPWHRYE
                     QIGNYEIMLIVATEHGCTDTTFRNVVVNGEFSFYAPTMFTPNGDGKNDCFRICGNGID
                     ANTFYMMIYDRWGNKVFETETYNPNVICKSCAEGSWDGTNKGDVDKGDTILENGVYTW
                     YCEFKDWHGTIYHKTGNVHLVR"
     gene            complement(7181..7696)
                     /locus_tag="PLK75_10585"
     CDS             complement(7181..7696)
                     /locus_tag="PLK75_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39732.1"
                     /translation="MLAEILTITIPPLLVLLTAYLVIDKMLKRDAENRKLEVSLKNQS
                     IITPIRLQAYERVVLLLERISPNHLVMRIQQPGMTVNELQREMLVNIRAEFEHNLSQQ
                     LYLRPETWDIVKIAKENTIKLINTCAAGLDPSDEAMVLTKSIFDKLIELTKAPNQVAL
                     DTIKKEINELF"
     gene            7779..8972
                     /locus_tag="PLK75_10590"
     CDS             7779..8972
                     /locus_tag="PLK75_10590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY39733.1"
                     /translation="MEKASDRLNKLAVSATLAMTNKSRELKEQGIDVINLSIGEPDFN
                     TPDYIKEAAKKAIDENYTHYPPVPGYTELRKAVVNKFKRENNLDFTVDQIVVSTGAKH
                     SIINVFLSVLNPGDEVIVPAPYWVSYVDMVKLADGEVIEIPCSIEQDFKMTPAQLKSA
                     LNEKTRALIFSSPSNPTGSLYTRDELRAFADILQQYPRVIVISDEIYEHINFVGPHQS
                     IAQFENIRDRVVVVNGVSKGYAMTGWRIGFIGAPLWIAKACVKLQGQFTSGACSIAQK
                     AAEAALNAGNHACKEMTSIFKKRRDAVLQQMKDIPGMKTNVPQGAFYVFPDISYFFGK
                     SDGECTIKTSDDLALYLLNKAHVATVAGEAFGAPECLRLSYATSEEEMSKAIDRIKKA
                     LALLR"
     gene            complement(9191..>9420)
                     /locus_tag="PLK75_10595"
     CDS             complement(9191..>9420)
                     /locus_tag="PLK75_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOY39734.1"
                     /translation="GDNENAIKIQIYCVLIANLLMTVVQKMLKRSWAFSNLVSFCKIH
                     LFNYIHLLRFLENPDKDWQKEQVELHQLSLF"
BASE COUNT         2692 a         1845 c         2011 g         2872 t
ORIGIN      
        1 gcaggcactt tggaattgac accacttact acaacaacct atacagtttc ggtagtggac
       61 gcccataatt gtgaaagtaa taccgtatcg atgaccataa ccgtgagtcc tcatttggaa
      121 ataactgagg tattacttca acaaaacaga tgttttcact cctgcgatgg cagtgctgag
      181 ctggttatta ttggcggaat tcctccgcta aactatagct ggggctctac caatcacata
      241 tataataacc tttgtgcagg aatttataca gttactgttt cggatcagat tggttgtttt
      301 gctaccactt attttctcat tactgaacct gatacattaa cctattcaat aaatactgta
      361 gatgccagtt gctatggctc agaggatggt acagcaaata ttattgttgc cggcggtacg
      421 cttccctatt catttttgtg gccaaacaat gcaacaacat cttttataac cacaggtgca
      481 ggtgattttg ttgttactgt taccgatgcg catagctgca gaatcgaagc atcatatact
      541 gtagggcaac ccactgaatt ggttgttcaa cctgcaggaa acccacaaat ttgcattggg
      601 caggaggcaa ctgtctcagc gcaggctgca ggaggaacgc cttattacga tttccactgg
      661 acaggtagcg atggcacaac atacccaacg catttgtttc atgtcagccc tgatactaca
      721 actatttata ctgtgaccgt agttgattca catggttgtt taggaaatgt tgccacggtt
      781 actgtaaatg tttacccacc tgttgaaatt gaatcggtaa ctaccagcta cgacacggtt
      841 tgtcaggggg aaagggccat tatttatgta aatgtgattg gagggaatgg aggcccctat
      901 attatcaacc ttgaaaatgg tgctacggtt ggctccccat ttactgtatt tccgggattt
      961 acatcaacct atcatgtaac agcatccgat atgtgtggat ctccatcggc aagcgattca
     1021 atcactatca cggtaatgcc atttccggaa gtaagtttta ctgccgacca cgttaatgga
     1081 tgttcacctg ctgttgtgca ttttacctcg ttgggtaacg atgaaaatta ctcgtatttg
     1141 tgggactttg gtgattataa ttttgcagat atacaaaacc cgatgcatat ttacaacagt
     1201 gcaggtacct atgatgtatc gctgattgtt acttcaccgt atggttgcca gaccagttca
     1261 gtaaccaatg atttgataac agtacatcca acacctacgg catcttttta tcttgaacca
     1321 gaaatagcta cactgcttga atcggaagtt gttttcacaa acttgtcgca ggatgcaatg
     1381 cgatacttct ggtattttgg agacggcgac agttctctgt tcgtgcatcc gaggcaccag
     1441 tatgccgatg ttggtgagtt tgaagtatat cttgtggcag aatcggagtt tggttgtcgc
     1501 gatactgtaa tgcggagagt ttatgtggaa aacataatta ctttctatgc tcccgaaaca
     1561 tttaccccca acggagatgg tatcaacgat tgcttccggg tttgcggaaa cggaatcgac
     1621 cccaatcatt ttgcattaag cgtttacgac cgatggggag aatgtgtctt tagtacagag
     1681 ttttatgata ccgaagtgag ttgcgatgca tgcagtagtg gatcttggga tggtaccgac
     1741 aacggaaacc gcctcgccgg tgataaatat ctccctatgg gattatatac ctggtattgc
     1801 gagtttaaag atgtgaataa tattacttat aagaaatccg gtaaaatcag gctgatacgt
     1861 taagcgttag agttcttaga tatttgaaaa ttgaacagaa aagaatgatt ctaattttaa
     1921 atcggtatgt gatgtttaga acaaattgta acacgtttta taacataaat aaaaaagaat
     1981 tacctttaca ggttaggaat catagtgaag agcaaaccat ttacaataaa tatcgtcttg
     2041 aattaaacta aataattatg aagcactttg cattgttttt tttgttgtta ttatcatttg
     2101 ctgcttattc gcaaagcgat gatgaagtaa ttcccgattc acggttgtat gtccgattta
     2161 gtgccgatta catcaatgtt atgttgcaat ctgatcctaa atcagttgaa tacctgaact
     2221 ttgaacttga ctttggctac aaaattatgg acgccggact tgagaaatgc gaaggattac
     2281 catatttaca ctacttcaat tctactgata aaaccgtaga aggtatagtt gaagacatca
     2341 atgaatcatc catcaatatt atggaatatc attacgacag gaattacgac aagcctaata
     2401 tttaccgtat cggtaacaca gggcaagtta ttatttttta ttcgttgaaa gatcttaacc
     2461 ggaaatttaa tcaaaactaa gatgaaaaga atttcaggaa ttattattat tatactcctg
     2521 cttggtatac tgggtaactt gaaagctcag acctacctga tcagtcaggg aggaacgatt
     2581 acaacctgtt caggcacgtt gtatgattct ggtggtgcca atggtgagta cggaacttac
     2641 gaagattaca caattaccat ctgcagtagc actggagggt caatttcact attatttaca
     2701 gtttttcagc tggagtctgc tacttacgat catttatcga tttacgacgg accgaatact
     2761 tcggcaaatg acattattta tcatgctggt ggctcaacat tacttaatca ggaaattacc
     2821 ggaagtggca attgcctgac tttagtatgg cacactgatg gatcggttac ttatccgggt
     2881 tttgctgcaa ctatttcctg cacttatcca tgtcaggatt atactattga tattgtaagc
     2941 tcaaaccctc cgcagagtcc tcctgctgat tcgctctggg ttgatgtttg tcagggtgaa
     3001 accgtaactt ttactgccac cgctgatttc tctaataata atgttgatta caatcaaact
     3061 ttggcaaaca caacctggta ctggacggtt gtgtcggagg ctggtaccga ggacttaggt
     3121 ggcttggggc aaaacacgct cacctatact ttcaccaacg aaggaggcta ttatataaat
     3181 ctccagaccg tggatatgaa tggctgtact caaattctta acgattactg gcgggttagg
     3241 gcttcgctta cacctgattt tacagtgaca cacccggcaa ctgtgtgtcc tggtgagact
     3301 ttcgacctta ccggtaccgc gagtgtggac ccatgggtac tgcaaatacc ggaagttgct
     3361 tttattgaag tttgtttcga agacgttgtt ggtgtcgatc aggaacaatg ctttgttcac
     3421 agtgcttttg caccggggca gtttattact gcagcttctg atgtggaatc gatttgtatg
     3481 aatatggagc attcctatat gggagacctg gatatttgga ttgaatgccc gaacggacag
     3541 actgcaaacc tttttgaaca ggcctgcggg ggtacctatt ttggcgaagc taacgataat
     3601 gatgattgcg ttcccggtgt tgggtggaat tattgctgga gcatgtcagg gtcttcgctg
     3661 atgtctgcta attgttatac tggatcctct ttacctgccg gaacttattt gcctgttggt
     3721 agcttttcat cgttgattgg ttgcccgttg aacggcgagt ggtgcattca cttcctggac
     3781 aacctgagta tagacgatgg aaatgttttc actgtagagc tccactttgc agattatttg
     3841 attccgagcg ctgaaaacat gtggacttat accaccacat ataacacttc gcctacttca
     3901 actgatcttg aatggacagg agaaggcgta ggagctaaca cgggaggagt taccaccgcc
     3961 tcatctctta caccagggag tcaacccttt acgttttcgg caaccgataa tttagggtgt
     4021 acttacgata ctacagtgta tgtaacggta cttcctttca acgatcctac atgctgtgtt
     4081 ttccctgatc ccaatgccgg aaacgatgat caggtttgtt caaactcata tacttttaat
     4141 ggaaccatca ctcagggcaa taccgcaaat tggacattgg taagtggacc tggaacaggt
     4201 acatggacga atcagacatc tcctgctgca acggtaacag taagcgctta tggtacttat
     4261 gtgtttcagt tatatgagca gaatttgtcg ccttcctgct ctgcaactga tcaagttacc
     4321 attgtctttc tcgaaaatcc acaatcaaca tttacagtaa ctacaatccc ttgttttggt
     4381 ggaaatacaa cagtaactta cacgggcaat gcccaatcta cagctacata taattggaat
     4441 tttaacggag ctcaggtaat cagcgggagc ggcgctggtc catatgttct ttcgtggact
     4501 actgcaggag cacatcccgt gagcctacag gtagtacaag atggttgtac atcaaccaac
     4561 acaacaaaca gtgttatgat gcctgcagtt ctctctcatg ttcttactac ccacaatgat
     4621 ccttgttatc aaagttgtca gggtaatgca tctgttttag taactggtgg aacagcccca
     4681 tatacgtatt catgggcttc gggcaataac tatttaccaa acctctgtgc aggaaactac
     4741 gccattacag taaccgatgc aaatggatgt actacagttg agagcttttc gatatcggag
     4801 cctacacaaa tggtaatgac ctcatatcag actacaaatc tatcgtgtta caacgctgct
     4861 gatggagcaa ttgcagttgt ggctcagggt ggaacaggca accttatgta tatttggagt
     4921 gatatcggtg taggtgaacc cacacgcgta ggtatgagtg ccgggaacta ttctctcaca
     4981 attatggatg aaaataattg ccaattagtg gaattctttc agttgacaca acccgatgaa
     5041 ctactgttaa caatttcgaa tagcttatct atttgcgaat ggcagacagc acatattgtt
     5101 gctcaacata tgggaggaac agctccgtat agctttttct gggataccgg tacgggttac
     5161 gaaattggtc cgaatgaatt gaatactgta ccccatcaaa caactaccta caatgtatat
     5221 gttcaggatg gtaatggttg taagagcaat acagcctcaa tgacggtaac tgttagtccg
     5281 gaactaatta ttgatacgat tttacttcag cataaccggt gtttccaatc ctgtgacggc
     5341 agagctgaac ttcaaataac cggaggcatt ccacctcttc agtacagttg gggatcagcc
     5401 gataacgtgc ttgctggatt atgtgcaggt atttataccg ttactgtcac cgatgaaatc
     5461 ggatgtacta ttaacgatgt atttacaatt acccaacccg acgaaataac ttacacgcta
     5521 aacactgttc ctgcatcgtg ctacaactac aacgatggtg aagcaactat ttatgttcag
     5581 ggaggcgtac taccatatac atatctatgg cctgacggtg acactgatat gacaatgtta
     5641 aatcattctg gagtatatat tgttactgtt acagatgatc acaactgccg gattgaagtt
     5701 ccgattacaa ttacccagcc cgatgatata ttactcattt catcagatga taagcagatt
     5761 tgtattgggc aaaatgcgat actttcagca caggcagtag gtggagtgcc atattacagt
     5821 ttcctgtggc aaggaaccga tggcagcaca ttcccgacag cttccattac agtaaaccct
     5881 gatgtaacta ccgaatattc ggtgactgtg accgactctc atggttgcat gggtaatgtt
     5941 ggtttagtga gagtgaatgt ttatcctccg ctaacaatta ccgatattgt aaccagctat
     6001 gacaccgttt gccctggtga acctgctatt attgaggttg atgcagctgg cggaaatggg
     6061 gggccatata tgcttctgct tgaagatggc tctgttgtac caagcccgtt tactgtgtat
     6121 cctcaggtta caaccatgta ttatgtgacc ttgatggatg cttgtggctc tccttcagca
     6181 gttgattcaa taaaaattaa tgtatatccg gcaccgccaa atttgtttgt ttccgacagg
     6241 gtgtcgggtt gtccgggagt tgccattaat tttacggaat taaatagtgt taacagcctt
     6301 tcatatttat ggaactttgg tgacaatggt tattctatcg agaaaaaccc tgcgcatatc
     6361 tatactgaac ccggggttta ttcggttagc cttacaacac gaacttcctt cgggtgttca
     6421 accacacaga caatacctta catgattact atcttcgaaa acccggttgc atcgttcaca
     6481 gtaacacctg agattgtttc ggtacttgat ggcgatgtgt ttttcgaaaa tctgtccagc
     6541 ttggcagact ctgtatattg gttttttgga gatggtgata gctcaattta ctatagtcca
     6601 tggcacaggt acgaacaaat tggaaattat gagataatgc ttattgttgc aactgaacat
     6661 ggttgcaccg acacgacttt ccgtaatgtt gtggtgaatg gagaattttc attctatgct
     6721 cccacaatgt ttacccctaa tggtgacggt aaaaacgatt gtttcagaat ctgtggtaat
     6781 ggtatcgatg caaatacatt ctatatgatg atttacgaca gatggggcaa taaagtgttc
     6841 gagaccgaaa catataaccc caatgtaata tgtaaatctt gtgccgaagg ctcatgggat
     6901 ggtacaaata agggtgacgt tgacaagggc gatacaattc ttgaaaacgg tgtgtatact
     6961 tggtattgcg agtttaagga ctggcacggt actatttatc ataaaacagg aaatgttcat
     7021 ttggttcgat aataaaaaag ccggctagcc ggcttttttg tttttaatat tcttcctgag
     7081 aattttagag ttctcgatat tccctgacgt aagtttgtgt tgtgcgtttg attattgcga
     7141 ttgtaagcaa attacctaat ttatgagtgc aggttatcag ctaaaacaat tcattaattt
     7201 cttttttaat tgtatcgaga gcaacttgat tgggagcttt ggtcaattct attaacttat
     7261 cgaagatcga ttttgtcaga accatagcct catccgaagg gtcaaggcct gctgcacagg
     7321 tattgataag tttaatcgtg ttttctttag caatttttac aatatcccat gtttccggtc
     7381 gcagatatag ttgctgcgac aggttatgct caaattcagc acggatattt accagcattt
     7441 cacgctgtag ttcgttcaca gtcatccccg gttgctgtat gcgcatcact aaatggttcg
     7501 gacttatacg ctcaagtagt aaaacaactc tttcgtatgc ctgaagtcgg attggcgtaa
     7561 taatggattg gtttttaagt gaaacttcaa gtttacggtt ttctgcatcc cttttcaaca
     7621 ttttgtcgat cacaagatac gctgttaata aaacaagtag cgggggaatg gtaattgtta
     7681 aaatttctgc cagcatgatg aagtaatttt atttatgctc tacaatatta gttaattata
     7741 aaataactgc gtaatttcgg caaagaaaac taaatattat ggaaaaagca tcagacagac
     7801 ttaataaatt ggctgtttca gccacattgg caatgacaaa taagagccgg gagcttaagg
     7861 aacaaggtat tgacgtgatt aatctgagta ttggagagcc cgattttaat actccggatt
     7921 atataaaaga agctgcaaaa aaggctatcg acgaaaatta tacgcattac cctccagttc
     7981 cgggatatac cgaactgaga aaggcagtgg tcaacaagtt taagagagaa aataatcttg
     8041 attttaccgt ggatcaaatt gttgtatcta cgggcgcaaa acattccata attaatgtct
     8101 tcctcagtgt tctgaacccg ggcgatgagg tgattgtacc tgcaccttat tgggtaagct
     8161 atgttgatat ggttaaactt gccgatggag aggtgattga aatcccttgc agtattgaac
     8221 aggattttaa aatgactccc gctcaattga aatcagccct caacgaaaag acacgagcat
     8281 taatattcag ctcaccttcg aatcctaccg gaagtctgta cacccgcgat gaactgaggg
     8341 catttgccga tattcttcag cagtacccga gggtaatagt tatttcagat gaaatttatg
     8401 aacatataaa ttttgttggt ccccaccaaa gtatcgctca gtttgaaaat atacgcgaca
     8461 gggtagtggt ggtgaacggt gtttcgaaag gttatgccat gaccggatgg agaattgggt
     8521 ttataggagc ccctctatgg attgcaaaag catgtgttaa acttcagggg caatttacct
     8581 cgggtgcttg cagcatagct caaaaagctg ctgaagctgc cttgaatgcc ggaaatcatg
     8641 cgtgtaaaga aatgacatcc atctttaaaa aacgccgtga tgcagtgttg caacaaatga
     8701 aagatattcc aggaatgaaa acaaatgttc ctcagggcgc tttctacgta tttccggata
     8761 ttagttattt cttcggaaaa tcggatggag aatgcactat taaaacatct gatgatcttg
     8821 ctttatatct tctaaataaa gcacatgtag ccaccgtggc cggtgaagct tttggtgctc
     8881 ctgaatgtct gcgtttatca tatgctacat cggaagagga aatgagtaaa gccattgacc
     8941 gaattaaaaa agcactggca ttgctgagat agtccctgag cttattaatt tagtttaatc
     9001 tcgcttatat tgtgattatt ccacttagct aaccggtgaa atctcaaagg tttattttat
     9061 tactgacacg atttctattt tttaacacta ttgtccgata aaaatatgga ataattttta
     9121 atttttgcta agttattgaa aatcaattaa tatatttcac aaaatcctaa taattaaaaa
     9181 gtaagccccc ctaaaacagt gaaagttggt gcaattcaac ttgctctttt tgccaatctt
     9241 tatcgggatt ttctaaaaat ctaagcagat ggatgtagtt gaacagatgg attttgcaaa
     9301 aacttaccag attcgaaaaa gcccatgacc ttttcaacat tttttgcact actgtcatta
     9361 gcagatttgc aattagcaca cagtaaattt gtatcttgat ggcattctcg ttatcgccca
//
LOCUS       DAOOQP010000116         9397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9079_length_9397_cov_5.542282, whole genome shotgun sequence.
ACCESSION   DAOOQP010000116 DAOOQP010000000
VERSION     DAOOQP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9079_length_9397_cov_5.542282"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLK75_10600"
     CDS             <1..308
                     /locus_tag="PLK75_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY39735.1"
                     /translation="INLLSVILGIIGGYGFNYITRLVSMPDFIYGLHYYFIPFYVTYS
                     LIKMVVFAIIITTVSAYHGYFTQGGALEVGRSSTRAVVYSIILILFFDLVLTQILLT"
     gene            305..1060
                     /locus_tag="PLK75_10605"
     CDS             305..1060
                     /locus_tag="PLK75_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY39736.1"
                     /translation="MIRVVNISKSFQKQQVLFNISAEFYPGKVNQIIGKSGSGKTVLL
                     KSIVGLHDILEGEIWIGDKEYSKASFSERRVIRQEMGLVFQGSALFTSLTVEDNVMLP
                     LNMFSKMSRAEKLERVNFCLNRVNIDNANKLFPAEISGGMQKRVAIARAIVLNPKYLF
                     CDEPNSGLDPQTSIVIDNLIKEITYEYNITTVINTHDMNSIMEIGDQIIFIFEGKNWW
                     QGSKNEIFTSDNQELNEFVFASKLYQKIKTVSI"
     gene            1057..1425
                     /locus_tag="PLK75_10610"
     CDS             1057..1425
                     /locus_tag="PLK75_10610"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOY39737.1"
                     /translation="MKLGRIHLIISIAVVCILWSCSSGAKRPGVPSGILNEKQMEDVI
                     YDLHKADGIMTSGVIPSSELYADSVIYHSVYLKHNTTSEQFQKSIMYYIRSDTKGLRL
                     VYSNVIERFNQDKTELMEWN"
     gene            1435..2616
                     /locus_tag="PLK75_10615"
     CDS             1435..2616
                     /locus_tag="PLK75_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY39738.1"
                     /translation="MKKLSAHYIFDGICLRKNAILEIEDSGKIVGLSEERKNIAEAEN
                     LSFYNGILLPGIINSHQHLELAWMKNRIAGSKGMAGFLNSVAELRKSTPEEPTISEEM
                     RTLDRIMYSQGITAAIDVSNNAESIGVKKDSNIEYFTTVELYGLDSCRNDEIVRKGEE
                     IVGQFRSKSLNASLTPHSFYLTNTELLKIISCIGNNMVSIHFFESNEEACFNVGNDNE
                     LSLFLRSFYPPFAIPENYSEIIVKTISKIIKTNKLILVHNTFIPEFLLKSLMALDNTE
                     NLFFCLCPKSNRYITERLPDLNKFISHGCKLLLGTDSPASNPDFNFASELFEIQQNYP
                     SVSIAEMLQWCTSNPAEIFCCKGNMGSFITGTSPGIVLLENAYLPNLSLTSSSIFRRL
                     F"
     gene            2667..3317
                     /locus_tag="PLK75_10620"
     CDS             2667..3317
                     /locus_tag="PLK75_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOY39739.1"
                     /translation="MNSFIKRNIVFAICVLLLSFVGMAVILITDKFQLHVILNNQHSV
                     ALDVFFKTITFVASGWAITICVLIFLFYNTKYALITAISGIAGSFLVQFLKRAIFAYH
                     RPSYYFDIMPGLNKIENYELYQNFSFPSGHATTAFTLFFVLSMVSGRWWLKMGLFILA
                     LVVAYSRVYLSQHFFEDVIAGAALGFVVAYCTAYIFDKSRFNRLELPVYKLFKSKQ"
     gene            3314..4954
                     /locus_tag="PLK75_10625"
     CDS             3314..4954
                     /locus_tag="PLK75_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY39740.1"
                     /translation="MRNIFDKNHVLLLLLGALLFIPFNGGVHLFDWDEINFAESAREM
                     IVTGNYLDVQINYEIFWEKPPLFIWMQVASMKIFGINEFAARFPNAICGIVTMLLLYA
                     AGKRLQNSKFGLFWSLTYITSLLPFFYFKSGIIDPWFNAFIFLSVWYIILYSGKFKGN
                     KLYFSFISALFLGLAILTKGPVALLVFALVYLIQLIYFRFKTFFRIKDLLVFILTLVF
                     IGGFWFILQIISGNIGAVQDFINYQIRLFSTQDAGHGGFLFYHFVVLFFGVFPASVIA
                     LRAFIKKKDTPDAVRYVKTWLIVLFLVVLILFTIVKTKIIHYSSLCYFPLSFLAAMQL
                     YRIAADKDKIRLFEKILLIFMGCIYAIGLAAFSNIEIIKTYLFKNHLIQDPFAIANLQ
                     ADAHWNGFEWLISLILLMGLVYFIANIGKQNKLKTLIVFGATLLVFHMGAMVLFVPRA
                     EEYTQKAAIEYFKELPEDECYVKTLGYKSYADLFYFNKPVPINKLSSDAVWLLYGNID
                     KPVYFISKCTRKEQILNENRHLQVIGEKNGFVFYKREL"
     gene            4959..5735
                     /locus_tag="PLK75_10630"
     CDS             4959..5735
                     /locus_tag="PLK75_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39741.1"
                     /translation="MAGFLFNDLIFGPVYSRRLGRSLGINLLPINAKWCNFDCVYCEC
                     GWSAHYNDSDVFPDSKQIVEMLENVLTDEIRKHERIDAITFAGNGEPTLHPDFAWIVE
                     NVVALRNRLMPDAQVAVLTNATMLHKKNINDALQRADKAILKLDTAIQRSYNLINQPF
                     DAVSVEAVIENIKLFPGDIYIQTMFFTGENKGVAIDNTSEEELKMYFAALKYIQPKQV
                     MIYSIDRATPGSGLQKVAVGKLEEISQKIQSLGFEVLLTP"
     gene            5732..6817
                     /locus_tag="PLK75_10635"
     CDS             5732..6817
                     /locus_tag="PLK75_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOY39742.1"
                     /translation="MKKATVNILYGVLDWGLGHATRSIPVIRYLRDCNHRVILVGNGQ
                     SLQLLKHHFPELETIDFKCPEIRYGKSKSYILKLVVMAPYYMALFRRQYYRTRKIVED
                     KNIDLVISDNRPGLRSHLCKSIYITHQITPDFGRWSGMLSKIASQLHQAIIKKYNSCL
                     VPDNAYEPSLSGKLSHCHELQNVQYIGILSRFATYKAEVDKKEQILLLLSGPEPQRTI
                     FEEILLSMFSKCEDAVILVRGTYKKTERAYSPKFTVIDIAEDELLYKLIAESSLIFCR
                     SGYSTLMDLALCRKKAIIVPTPGQSEQEYLAKMLEEKYGFTTVSQHSLPNFEWKLAER
                     SMEWNFPTQSLFKEKICSIIDQIIKKR"
     gene            6814..7689
                     /locus_tag="PLK75_10640"
     CDS             6814..7689
                     /locus_tag="PLK75_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39743.1"
                     /translation="MKVTGFSFIRNALKYDYPVTEAILSVLPLCDDFVVAVGKSEDGT
                     RNLIDSVNPTKIKVIDTIWDDTLREGGRVLAVETDKAFQAIPCDSDWCFYIQGDEVVH
                     EKYLNTIREAMLRYKDDERVDGLLFKYLHFYGSYDYVGESYRWYRNEIRVIRNNKKIY
                     SYRDAQGFRKDDNKVLIVKPVDAYVYHYGWVKDPRAMQLKQEDFNKLWHDDDWVAQNV
                     VKADEFDYAKIDALSLFTDTHPAVMKDRILRKNWKFDYDISFNTHKFKDRVKRFAEKK
                     LHVKFGQYANYKILK"
     gene            complement(7746..8528)
                     /locus_tag="PLK75_10645"
     CDS             complement(7746..8528)
                     /locus_tag="PLK75_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39744.1"
                     /translation="MKFKNFTSSLLLLFIILVPLLNGCQKGDEDPFFSIYSRKARVVG
                     DWVVNYYKSDVKTISGIEGTDQIRTLVEVNALNWEENVYILGTDSVRETKGKITVDNN
                     GRARFNYNFNSNGIMTSIYEYQVIEEVSDEDMGTDTTITTTIKIEYSGTWNFLAGVDD
                     YKNKERLALVIQEEKYLKIVTKYTVQEDDEYGGVTTTTREAYTKRFANGEMSTIWVLR
                     MLKNKEMIMDQNVNDLYVVTDINGTGTSISQVGTRTQTLQLD"
     assembly_gap    8667..8676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8952..>9397
                     /locus_tag="PLK75_10650"
     CDS             8952..>9397
                     /locus_tag="PLK75_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39745.1"
                     /translation="MVINNKELSTKIRLGIMMFALLCLALIVFSLIYAWVPNHLIEII
                     STCVFVLFAVFILIRKYCYILFNSEGSKIILRYSPLQPLSHGNFSIEIPKKDFKNFVI
                     TKSLFGLRRNLIMFVETPQGVAKFKPVSVTSLSKKELTDLIESLQS"
BASE COUNT         2883 a         1595 c         1887 g         3022 t
ORIGIN      
        1 ttatcaattt gttgagtgta atccttggta ttattggtgg ttatgggttc aattatatta
       61 cccggcttgt gagcatgccc gattttattt atgggctaca ttactatttt ataccctttt
      121 atgtaaccta ttcattgatt aaaatggttg tatttgctat tataatcaca actgtatcgg
      181 catatcatgg ctattttact caggggggag cactcgaagt tggccgtagc agcacccgtg
      241 ccgtagtgta cagtattata ctgattctgt tttttgattt ggttcttacc caaattctat
      301 taacatgata agggtggtaa acatatcgaa aagctttcag aaacaacagg tattgtttaa
      361 tatatctgct gagttttatc cgggaaaggt aaaccagatt atcggtaagt ctggctccgg
      421 aaaaaccgta cttcttaaat cgattgttgg cctgcacgat attcttgagg gagaaatctg
      481 gattggtgac aaggaatatt caaaagcctc tttcagcgaa aggagggtta taaggcaaga
      541 gatggggttg gtttttcagg ggagcgcatt gttcacatca cttacagttg aagataacgt
      601 aatgctgcca ttaaatatgt tctcgaaaat gagcagggca gaaaaacttg agcgtgtcaa
      661 tttttgtttg aacagggtga atattgacaa tgccaacaaa ttgtttcctg ccgaaataag
      721 cggtggtatg caaaaacggg ttgctattgc cagagctatt gtgctgaatc caaagtactt
      781 gttttgcgat gaacctaata gcggacttga cccacaaaca tctatagtaa ttgataatct
      841 tattaaggaa attacatacg agtacaacat tacaacagtt atcaataccc acgatatgaa
      901 ctcaatcatg gaaattggcg accagattat cttcattttc gaaggtaaaa attggtggca
      961 gggtagcaaa aacgagatat ttacatcgga taaccaggaa ttgaacgaat ttgtatttgc
     1021 atcgaaacta tatcagaaaa ttaaaactgt cagcatatga aacttggcag aattcatttg
     1081 attatttcaa ttgctgtagt ttgtatttta tggtcgtgta gctccggtgc aaagcgcccc
     1141 ggtgtaccaa gcggtattct caacgaaaaa caaatggaag atgtaattta cgatcttcat
     1201 aaagcagatg gtattatgac ctccggagtt attccttctt cggaactcta tgctgattct
     1261 gtgatttatc attccgttta tttaaaacac aataccacca gtgagcaatt ccagaaaagc
     1321 attatgtatt atatccgctc cgataccaaa ggattacgat tggtctattc gaatgtaatt
     1381 gagcgtttta accaagataa aaccgagtta atggagtgga actaatttgg agttatgaaa
     1441 aaactgtcgg cacactatat tttcgatgga atctgcttac gaaagaatgc aatactggaa
     1501 atagaggaca gcggaaaaat agtcggactt tccgaagaga gaaaaaatat cgctgaagct
     1561 gaaaaccttt ctttttacaa tggaatatta ctgcccggaa taatcaattc gcaccagcat
     1621 cttgaattgg cctggatgaa aaaccgaatc gcaggttcaa aaggtatggc gggtttcctg
     1681 aattcagtgg cagaattgcg aaaatcaaca ccggaggaac ccacaatatc cgaagagatg
     1741 agaacattgg ataggattat gtacagccag ggtattactg ctgctattga tgtttcgaat
     1801 aatgcagaaa gtattggagt aaaaaaagat agtaacatag aatatttcac aacggtagag
     1861 ttatatggtc tggatagttg tcgaaatgac gaaattgtcc gaaaaggaga agaaattgtc
     1921 gggcaattca gatcaaaatc cctcaatgct tcacttaccc cgcattcttt ctacctgacg
     1981 aacaccgaat tattaaaaat cattagctgt attggcaaca atatggtttc aattcatttt
     2041 tttgagtcaa acgaagaggc ttgtttcaac gtaggcaacg acaatgagtt gtcgctattt
     2101 ttgcgaagct tttatcctcc attcgctata ccggagaatt attccgaaat tatcgttaaa
     2161 acaatatcga agattataaa aacgaataag ctgattcttg ttcacaatac ttttatccct
     2221 gagttcttac tgaaaagtct gatggcattg gacaacacag aaaatttgtt tttttgcctc
     2281 tgccccaaaa gtaaccgata tattacagaa agactacctg atctcaataa gtttatttcg
     2341 catggttgta agttactgct tggaaccgat tctcccgcaa gtaacccgga ttttaatttt
     2401 gccagtgagc tatttgaaat acaacagaat tatccgtcgg tatcaattgc tgaaatgttg
     2461 caatggtgta cctcaaatcc ggctgaaatt ttttgctgca aaggtaatat gggctctttc
     2521 attacaggca cttcgccggg aattgtattg ctcgagaatg cttacctccc taatttatca
     2581 ttaacttcat catcaatatt cagaaggctt ttttaattct tttgacttat attattaatt
     2641 ttgggtgatg tttaattgca ttcgatttga atagcttcat aaaaagaaat attgtatttg
     2701 ctatttgtgt tttactgctc tcatttgttg ggatggcagt gattttaatt acggataaat
     2761 tccaattgca tgttattttg aacaaccaac actcagtagc attagatgtg ttttttaaga
     2821 ctatcacctt tgttgcatcg ggttgggcta ttacaatttg cgtgcttata ttcttgtttt
     2881 ataacacaaa gtatgctctt atcaccgcaa tttcaggaat agcaggaagt tttttagtgc
     2941 agtttcttaa acgcgcgatt tttgcttatc atagaccttc atattatttt gatattatgc
     3001 ccggcttgaa caaaattgaa aattacgaac tgtatcaaaa tttcagcttc ccttcaggtc
     3061 atgcaacaac ggcgtttaca ttgttttttg tgctatctat ggtaagcgga agatggtggt
     3121 tgaaaatggg tttgttcata ttggccttgg ttgttgctta ttcaagagtg tacctttcgc
     3181 aacatttctt tgaggatgta attgcaggtg cagcattggg cttcgtagta gcttattgca
     3241 cggcttatat tttcgacaaa agccgattta acaggcttga actaccagtg tataaattgt
     3301 ttaaatcaaa gcaatgagaa acattttcga taagaatcat gttttgctac ttctactggg
     3361 agcattactt tttattcctt ttaacggagg ggttcatttg tttgattggg atgagattaa
     3421 ttttgcagaa tcggcacgcg aaatgattgt aacgggcaat taccttgatg tgcagattaa
     3481 ttatgaaata ttttgggaaa aaccgccatt gttcatctgg atgcaggtcg catcgatgaa
     3541 aatttttgga atcaacgaat ttgctgcgcg ttttccaaat gctatatgcg gtatagttac
     3601 catgttgtta ctatacgcag ccggcaaacg acttcagaat tcaaagttcg gattgttttg
     3661 gtcgcttacc tatattacca gtctgttgcc atttttttat tttaaatcag gaattattga
     3721 tccctggttt aatgcattta tttttttaag tgtatggtat ataatacttt atagtggtaa
     3781 attcaaaggg aataaactgt atttttcttt tatttcagcc ttatttctcg gactggctat
     3841 tctcaccaaa ggaccggttg cgttgttggt gtttgccttg gtgtatctga ttcaattaat
     3901 atatttccgt tttaaaacct ttttccgtat taaagacctg ttggttttta ttcttacact
     3961 cgtatttatc ggagggtttt ggtttattct tcagataata agtggaaata ttggtgctgt
     4021 ccaggatttt atcaattatc agatccgact tttttcgact caggatgctg gtcacggtgg
     4081 attcttattt tatcattttg ttgtgctgtt ttttggagta tttccggcat ctgtaattgc
     4141 tttgagggct tttataaaaa agaaagatac tcctgatgct gtacgctatg tgaaaacctg
     4201 gctgattgta ttgtttcttg tagtgctgat attatttacc atagtcaaaa caaaaattat
     4261 tcattactct tcgttgtgtt atttcccttt gagctttctt gctgcaatgc agttataccg
     4321 gattgctgcc gataaagaca aaatcagatt attcgaaaaa attcttttaa tttttatggg
     4381 atgtatttat gccattggtt tggctgcgtt ttcaaatatc gaaataatta aaacatattt
     4441 gttcaaaaac cacctgattc aggatccttt tgccatagca aatttgcagg ctgatgcaca
     4501 ttggaatgga tttgagtggc tcatttcgtt aatactactg atggggcttg tctactttat
     4561 tgcaaatatc gggaaacaaa acaaattgaa aacacttatt gttttcggag ctactttact
     4621 tgtattccat atgggtgcaa tggtactttt tgtccccaga gccgaggaat atacgcaaaa
     4681 ggcagctata gaatatttta aggaactacc tgaagatgag tgctatgtga aaacactggg
     4741 ttataaatca tatgctgact tgttttattt caataagcct gttccaatca acaaactttc
     4801 atcagatgca gtgtggttgt tgtatggcaa tatcgataaa ccggtatatt ttataagcaa
     4861 atgcacccga aaggaacaga ttttaaacga aaatcgacat ttgcaggtta taggtgaaaa
     4921 aaatggtttt gttttctata agcgtgaatt gtaaatctat ggcaggtttt ttatttaacg
     4981 atttgatttt tggccctgtc tattccagac gcttggggcg ttcgctgggg ataaatctgt
     5041 tgccgataaa tgcaaaatgg tgtaatttcg attgtgtata ttgcgaatgt ggctggtcgg
     5101 cacattacaa cgactccgat gtttttcccg attcaaagca aattgtcgaa atgcttgaga
     5161 atgtacttac cgacgaaatt cgaaaacatg aaagaattga tgccattacc tttgccggca
     5221 acggtgagcc aactttgcat cctgactttg cctggattgt cgaaaatgta gttgcgctac
     5281 gaaaccgact gatgcccgat gctcaggttg ctgtattaac aaatgcgacc atgctgcata
     5341 aaaaaaacat aaatgatgct ttgcaacggg cagataaggc tattctgaaa cttgacactg
     5401 caattcaacg aagttacaac cttataaatc agcctttcga tgcagtatcg gtagaagcag
     5461 ttattgaaaa tattaagctg ttcccgggcg atatttacat tcaaaccatg ttctttacag
     5521 gcgaaaataa aggagtcgca atcgataaca catcggagga ggagttgaaa atgtattttg
     5581 ctgccctgaa atatattcaa cccaaacaag taatgattta ttccatcgac agggcaactc
     5641 cgggcagtgg tttgcaaaaa gtcgctgtgg ggaagctgga agaaatttcc caaaaaattc
     5701 aaagtcttgg atttgaggtg cttcttaccc catgaagaaa gccactgtaa atatattata
     5761 tggtgttctc gactgggggc tggggcatgc tacccgatcg ataccggtta tcagatatct
     5821 cagggattgc aaccatcggg taattcttgt cggtaacggc cagtcgctgc aactacttaa
     5881 acatcatttc ccggaattgg aaacgattga ttttaaatgt cccgaaatca gatacgggaa
     5941 aagcaaatca tatattctga aactggttgt tatggcacct tattatatgg cacttttcag
     6001 gagacagtac tatcggaccc gtaaaattgt tgaagataaa aatattgact tagttatttc
     6061 ggataaccgc ccagggttaa gaagccattt atgcaagagc atttatatta cacatcagat
     6121 tactcctgat tttggcagat ggtcagggat gctgtcaaag atagcatcgc agctacatca
     6181 agcaatcatt aaaaaataca actcatgttt ggtcccagat aacgcctatg aaccgtcttt
     6241 gtcgggtaag ctttcgcact gccatgaatt gcagaatgtt caatatatcg gtatactcag
     6301 caggtttgcc acgtataaag ctgaagtgga taaaaaagag cagatattgt tgctattaag
     6361 cggacctgaa ccccagcgta caatttttga ggaaattctt ttatcgatgt tttcaaagtg
     6421 tgaagatgcg gtaatattgg ttagaggaac ttataaaaag actgaaagag cctattcacc
     6481 gaaatttact gtaattgata ttgccgaaga tgagcttctt tataagctta ttgctgaaag
     6541 tagccttatc ttttgccgta gtgggtattc cacgcttatg gatctcgcat tatgccgaaa
     6601 aaaggcaatt attgtcccca cacccgggca atcagagcag gagtatctgg caaaaatgct
     6661 ggaggaaaaa tatgggttca ctactgtaag tcagcacagc ttaccaaatt ttgaatggaa
     6721 acttgctgaa agaagcatgg aatggaactt cccgacccaa tcgttgttta aagagaaaat
     6781 ttgtagtatt attgatcaga taattaaaaa gagatgaaag ttaccggttt tagttttatc
     6841 aggaatgctt tgaaatatga ttacccggtg acagaagcaa tattgtcagt gttgccttta
     6901 tgcgacgatt ttgttgttgc ggttggaaaa tcggaagatg gtacacgaaa tctgatagat
     6961 agcgtgaacc ccacaaaaat aaaagttatc gatacaattt gggatgacac attgcgtgaa
     7021 ggcggcagag tattggcagt ggaaaccgat aaagcatttc aggcaatccc ctgcgatagc
     7081 gactggtgct tctatattca aggtgatgaa gtagtccatg aaaaatatct caatacgatt
     7141 agggaggcaa tgctaaggta caaagatgat gaaagggtcg atggtctatt attcaagtac
     7201 ctgcattttt atgggtcgta cgactatgtt ggagaatcgt atcgctggta caggaacgaa
     7261 ataagggtaa tcagaaacaa taaaaagatt tactcctatc gtgatgctca gggctttcgt
     7321 aaagatgaca ataaggtgct cattgtgaaa ccggttgatg cctatgtata tcactatggc
     7381 tgggtgaaag atccacgtgc aatgcagctc aaacaggaag atttcaataa gctatggcac
     7441 gatgatgatt gggtagcaca gaatgttgtt aaagctgatg aatttgatta tgctaaaata
     7501 gatgccttga gtttatttac tgatacgcat cccgcggtga tgaaagacag aatactccgt
     7561 aaaaactgga agtttgacta tgatatcagt ttcaacacac ataagttcaa ggatagggtt
     7621 aaacgttttg ctgagaagaa attgcacgtg aaattcggac aatatgcaaa ttataagatt
     7681 ctgaaataaa aaagaggctg ttcttctgaa acagcctctt tttttgagta aattcaataa
     7741 aagaattagt cgagttgtaa agtctgggtt ctggttccta cctgagaaat agaagtaccc
     7801 gttccgttaa tgtcggtaac aacatacaaa tcgttcacat tctgatccat tatcatttct
     7861 ttattcttca gcatacgcaa cacccaaatg gtagacattt caccgttggc aaaccttttt
     7921 gtgtatgctt ctctggtggt tgttgtaaca cctccgtatt catcatcttc ctgaacggta
     7981 tatttggtaa caatcttcag gtatttttct tcctgaatta caagtgctaa cctttcttta
     8041 tttttgtaat cgtcaacgcc agccaggaaa ttccatgtgc cggaatactc tattttaatg
     8101 gttgtggtaa tggttgtgtc ggtacccatg tcttcgtcgg aaacttcctc gattacctga
     8161 tactcataaa tagaagtcat gattccgttg ctgttgaaat tgtagttgaa acgagctctt
     8221 ccattattat caacagtaat tttacctttg gtctctctta cactatcagt accgagaata
     8281 taaacattct cttcccagtt taaggcgttc acttcgacca aggtcctgat ttgatcagtc
     8341 ccttcgattc cgctaatggt ttttacatct gatttgtaat aatttacaac ccaatcccca
     8401 actactctgg cctttcgtga gtaaattgag aaaaacgggt cttcatcacc tttttggcat
     8461 ccgttcagca aaggaacgag aataataaaa agcagtaata atgaactggt aaaattcttg
     8521 aatttcatag gcaatttact ttcagatatc aactaacaaa attaatcgaa ttaattacaa
     8581 atacaaaaaa agcaattttt tttaaatata cataacattt cataaaaaaa ttaacaaaaa
     8641 aaaataatgt ctgttaataa aaagagnnnn nnnnnnccat cacagtcatt atctttatta
     8701 caagaaaata aaacaattat taacaaaaaa ctgaaagtga aattgaacat ttaagtcatt
     8761 agtaaaagta ctgtaaattg gggtataatg caaagaaaaa tcataagctg gcagcgaaat
     8821 aaagaatatt tgttgatgtt tttaaagtaa ggcgtcaatc gtataattaa aaaggttttg
     8881 aagtgtgctt gaaaatcggt tattttgcat agaatattta aacgtaatag tctatatcaa
     8941 attgcaatgc tatggtaata aacaataaag aactttctac aaaaatccga ctgggaataa
     9001 tgatgtttgc gttgctatgt ctggcactta ttgtttttag ccttatttat gcatgggttc
     9061 ccaatcacct gattgaaatt atcagtacct gtgtttttgt tttatttgcg gtgtttattt
     9121 taataagaaa atactgctat attttgttca acagcgaggg tagcaagata atactgagat
     9181 attctccgtt gcagccattg tcgcatggga atttttctat tgagataccg aagaaggatt
     9241 ttaaaaactt tgtaataacg aaatctttgt ttggattgcg tcgtaaccta attatgtttg
     9301 ttgaaacccc gcagggtgtg gcaaagttta aaccggttag cgtaacctcg ttgtcgaaga
     9361 aagagctaac agatcttata gaaagcttac agtcaat
//
LOCUS       DAOOQP010000117         9300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9197_length_9300_cov_6.554246, whole genome shotgun sequence.
ACCESSION   DAOOQP010000117 DAOOQP010000000
VERSION     DAOOQP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9197_length_9300_cov_6.554246"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1136)
                     /locus_tag="PLK75_10655"
     CDS             complement(45..1136)
                     /locus_tag="PLK75_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY39746.1"
                     /translation="MKILVRILAILHGLVCIPLLAAYLARYIDPLKIKWLVVATYAFP
                     WLLLLNALFIPLWLIFKRKMLWFAVPLLLLGTPMINGLIPLRNYFSATPSGEGHLKIM
                     TYNVHVFGLYNWRQKDITKNEMFRYIETEDPDIACFQEYFYDSGGFFPTTDSLLKILK
                     AKYYYQVFSAHSAKTQKFGIATFSKYPIIAKGSIRFEKTHNLCIYTDILYEKDTLRVY
                     NCHLQSLYFNDKNYRELENFDSSDIPKGNVGQYRNIVGKILLAAQRRSSQVKILRQHI
                     DSCQYSIIVCGDFNDNPFSYAYRIVSKGFKDSFSGAGSGFGYTWEQKGVKQRIDYVLY
                     SKDLVCSEHKITRVPYSDHYPLVVKIGKK"
     gene            1316..1951
                     /locus_tag="PLK75_10660"
     CDS             1316..1951
                     /locus_tag="PLK75_10660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY39747.1"
                     /translation="MLFICLLLSTSVFSQTTKVLIKTSKGDITVMLYDDTPLHRDNFI
                     KLVNESYYNDVLFHRVIKQFMIQAGDPDSRNAPAGKMLGNGGPGYTIAAEFVPTHIHK
                     KGALAAARTGDNMNPEKRSSGSQFYIVQGQVFTDMQLDSYEKQLGKVFTKEQRDAYCT
                     VGGAPHLDGAYTVFGEVTDGLDVIDKIAAVETNKANRPLEDVKIISVSIIK"
     gene            2004..2288
                     /locus_tag="PLK75_10665"
     CDS             2004..2288
                     /locus_tag="PLK75_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39748.1"
                     /translation="MASKKNLKQDINRIVNELVADCLQYMKMQPGKAETEIAEIISEA
                     LKMRKELIYKLNHLEDTEDAKKLKSQINEIAVTLIGKTDASYKTLSKLPR"
     gene            2288..3013
                     /locus_tag="PLK75_10670"
     CDS             2288..3013
                     /locus_tag="PLK75_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOY39749.1"
                     /translation="MQKILVSGDFGKIQILKKKGTKNIRLSVHPVTGVKISIPWYVSY
                     EEGVRFLHLKKDWVLAKQTEFKQMQTERKGFIGDGFEFAGYKLLFNHGDHAKISARIH
                     DKNIEVKLPMGSDLTQSFLQQQLHKFCEKALKIKASAKLPGRVEYLAKLHRFNYHSVK
                     IGKSRTRWGSCRSDNKLTFSCYLMLLPEELIDFIIMHELCHTVFKNHSHQFHALVNQI
                     CDNNEALLTKKLKQIKHPLMYAV"
     gene            3023..4342
                     /gene="miaB"
                     /locus_tag="PLK75_10675"
     CDS             3023..4342
                     /gene="miaB"
                     /locus_tag="PLK75_10675"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOY39750.1"
                     /translation="MQQKKVYIETYGCQMNVADSELVAAMLPDQKFAISASIENADVI
                     IINTCSVREHAEKKIWNRLQHIHGLKRKNKQLVTGVIGCMAQRLGEEILNHKTVDFVA
                     GPDAYRSISGLIEKALQGKKESDTHFSIDENYHNILPKRSDINHISGFISITRGCNNF
                     CSYCIVPYTRGRERSRDYTDILSQAEDLSKNQYREIYLLGQNVNSYQNNNLNFSALLS
                     LIADSYPDMRIRFTTSHPKDLTDELIEVIAKYKNICNHIHLPVQSGSNRILKLMNRRY
                     SREWYLSRVEKIRELIPGCGLSTDIFCGFCGETEDDHAETLELLRRVRFDSAFMFKYS
                     GRPGTLAAEKMADDIPEELKIKRLNELISLQNKISLQSNKNDIGKLFEVLIENESKRS
                     KDYFSGRTETNKTVVFEKDQTQIGQFVTVMITDASSATLKGKIINHG"
     gene            4335..4673
                     /locus_tag="PLK75_10680"
     CDS             4335..4673
                     /locus_tag="PLK75_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOY39751.1"
                     /translation="MDKVTEFANYRSEMNKRLLESDNKIIKRIFSLDSMTYTDSALSA
                     KVKEMLGLATSLVLRCDDCVKYHLEQCYKHKLSTDEIFDILAVCNVVGGTIVIPHTRR
                     AVEYWDLLNK"
     gene            4704..5216
                     /locus_tag="PLK75_10685"
     CDS             4704..5216
                     /locus_tag="PLK75_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39752.1"
                     /translation="MKSRFFYSIALILFATVLLFSCGKSAKKAAQYNEKIVQEQIKLQ
                     DNFNAFEATFADYKKEEMSSKLQEALKQADASLEFFTKLDDFDNQSAYKNAAIEFASM
                     YKRLATNEFPKLVDRYSLPDSLFKADVEIECFNLLQDIDSIYNESFTKLVKAQKEFAS
                     TYQFTIEEKK"
     gene            complement(5246..6508)
                     /locus_tag="PLK75_10690"
     CDS             complement(5246..6508)
                     /locus_tag="PLK75_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOY39753.1"
                     /translation="MQKVKTLLISIIFVICSAADIWAQHVEDTIKQLNHELARYRIIS
                     DSLENLLEDANLRMIAANLNMYGLPEIKQGEELIEHNGYILVFSQKHHQAAWVAHMII
                     SEVAESRVSRTNDFRPDPLVKGGTAVESDYFLRIVGPDSTITYDGFGFDRGHLVPSAD
                     FRWSQKALSETYLYSNISPQLPDFNREKWAELENTLRAYVIEGKSASVYVVTGPVFSD
                     SATFITKGVNKLPVPSYFFKAVFDKPNMRSIAFLMPHTKLEYPVEWYAITIDSLESFT
                     GIDFFASLDDSLESSIESQCDYKYWLTVEQKNDVKPLKETERPKNTYNTVEAALFSGT
                     NKNVCICGTVVSTHISAKGNVFINLDKSFPNQIFSISIFKKNLVNFSYLPHIYLQNKK
                     ICVKGLVKEYEGTPGIYPVNEKAIYEIE"
     gene            complement(6496..6756)
                     /locus_tag="PLK75_10695"
     CDS             complement(6496..6756)
                     /locus_tag="PLK75_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOY39754.1"
                     /translation="MDKIIKLPNIGVKLTERLSEAGFQSLSDLKSMGAEGVFLKLKTV
                     FPDACVSTLFAIEGAILGIRWHGIPEQRKRELKNFFNQTCKK"
     gene            complement(6775..8076)
                     /locus_tag="PLK75_10700"
     CDS             complement(6775..8076)
                     /locus_tag="PLK75_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOY39755.1"
                     /translation="MNRYSPIPADLFVRNRKKLYGKICSPAIILLFSSEKKLRSGDQY
                     YKYRQNSDLFYLSGIEQDDTVLVLCPSHPNTALHETVFVPAPDERNRIWYGDYMSQNE
                     ITDISGITNVKLLPELESYIGELLKFSSRIYVPYGVNENGAVIHSSCDNVMASKICHH
                     FNDCWFGSLNPILSDLRLVKEPEELMLIQKAISITSEAFQQALKTICPGKYEYEIEAA
                     ITSEFIRSGASDHAFPPIVASGSNACILHYNKNSGVLQEGDLVLLDFGAEYANYSADC
                     SRTAPVNGYFSERQKSLYNAVLSVSRQITSYVVPGNTILKLNEIAGELMQEELLKLGL
                     ISVADVAVQSPENAAYKKYFMHGVSHFMGLDVHDVGAKSDVFRKGMVLTCEPGIYIAK
                     EGIGVRIENDILVGDEPVNLMDGIPIVTEEIEDLMRQNIGK"
     gene            complement(8073..>9300)
                     /locus_tag="PLK75_10705"
     CDS             complement(8073..>9300)
                     /locus_tag="PLK75_10705"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOY39756.1"
                     /translation="DVNTTVKACRLLNARMLEFGWNFPQHLGVTEAGEGEDGRIKSAL
                     GIGSLLNDGIGDTIRVSLTEEPENEIRFAKKLVSAYVNRAPRIQNMPMDIAPYEVFKT
                     EIPGEGKELNARKCAIMVNSEVLPYLSAQHKEEIDFVFYCTGNGTGTGEKYVSPLHGI
                     NPTDEIYPYYNSAEVIAQLVQAKIPVGKRFFLEMNVGEFQKLKQFIVQTKLRISIVLN
                     AITQYPAGEIRYFYKQLLDAGLKVPLIIRYHHNKFYDDNAKAEMSLYPGSILVDRLAQ
                     GIFPMIEAQHNEHFDAILAMFQALGLRYTKAEFISCPGCGRTAYDIETIVKQVKIRFS
                     HLKGIKIAVMGCVVNGPGEMNDSDYGYVGSQKGIVTLYKGCVAVRKNIPENKAIDELV
                     SLIKENGDWCDPDYGV"
BASE COUNT         2873 a         1893 c         1794 g         2740 t
ORIGIN      
        1 aaaaactgaa atacaccata tgataataaa ccaaccaaag actttcattt cttaccaatt
       61 ttaacaacca acgggtaatg atcggaataa ggtaccctgg taattttatg ttcactgcaa
      121 accagatctt tactataaag cacataatcg atgcgttgct tcaccccctt ttgctcccaa
      181 gtatacccaa aaccggatcc ggcacccgaa aacgaatctt taaagccttt cgaaacaata
      241 cggtacgcat acgaaaatgg attatcatta aaatcgccgc aaacaattat gctatactgg
      301 catgaatcga tatgctgccg aagtatttta acctgcgagc tgcgcctttg tgctgcaaga
      361 agaattttac cgacgatgtt tcggtactgc ccaacattac ctttaggaat atcgcttgaa
      421 tcgaagtttt cgagttctcg gtagttctta tcgttgaaat acaacgattg taaatgacag
      481 ttgtacaccc gcagggtatc tttctcatat aaaatatcgg tataaataca caggttatgt
      541 gttttttcga aacggatact tccttttgca ataataggat acttgctgaa agtggctata
      601 ccaaattttt gtgtttttgc cgaatgtgca gaaaacacct gataataata tttggccttt
      661 aaaattttca gcagcgagtc ggtggtaggg aaaaaacctc ctgaatcgta gaaatactct
      721 tgaaaacagg caatatcagg atcttcggtt tctatatacc gaaacatttc gtttttggta
      781 atatctttct gtctccagtt gtacaaacca aaaacgtgca cattgtacgt cattatctta
      841 agatgccctt ctcctgaagg tgtagcagag aaatagtttc ttaaaggaat gagtccatta
      901 atcattgggg ttccgagcaa cagcaatgga acagcgaacc atagcatctt ccttttaaaa
      961 ataagccata acggaataaa taatgcgttt agcaacaata gccacgggaa agcatatgtg
     1021 gctactacca gccatttaat cttcagaggg tctatatatc tggccagata tgcagcaaga
     1081 aggggtatgc aaaccaaacc atggagtatg gccaatattc tgacaagaat tttcaactgt
     1141 caacagcttt gtattaagca aacaaaacta caatattcct tttaatattt gactctgttt
     1201 gctaaaatct tgagttttct actatctgct attggtatca atattgttat ttattatttt
     1261 tattactttt gtgcgaaatt aattggttat gaaatcggtt ttaaacatag cagccatgct
     1321 ttttatatgc ttactgttaa gcacatctgt tttttcacag acaaccaaag tgcttattaa
     1381 aacaagcaaa ggcgatatta cagtgatgtt gtatgacgac actccgttgc atcgcgacaa
     1441 cttcattaaa cttgtcaacg agtcgtatta caacgacgta ctatttcaca gagtaatcaa
     1501 acaattcatg atacaggctg gagaccctga ttcgagaaat gcaccggcag ggaaaatgct
     1561 tggcaatgga ggcccaggat ataccattgc tgcagaattt gtcccaacgc atatccacaa
     1621 aaaaggagcc ctggctgcag cacgtaccgg cgacaatatg aaccccgaaa aacgaagcag
     1681 cggcagtcag ttttatattg tccaggggca ggtattcacc gatatgcaac tcgattctta
     1741 cgaaaagcag cttggcaagg ttttcacaaa agagcaaaga gatgcttatt gtaccgttgg
     1801 aggggctcct catctcgatg gagcatatac ggtgttcgga gaagttacag atggtctgga
     1861 tgtgatcgac aagattgctg ctgtggaaac taataaagcg aaccgaccac ttgaggatgt
     1921 taaaataatt tcggtatcga taattaaata gttgtgattt tatttgaagt ataaaccaga
     1981 aattttgaaa ttaaccagcg attatggcaa gtaaaaagaa cttaaaacag gatatcaatc
     2041 gcatcgtaaa tgaactagtt gccgattgtt tacaatatat gaaaatgcag cccggaaaag
     2101 ctgaaacaga aatagctgaa atcatcagcg aagccctgaa aatgagaaaa gaattgattt
     2161 ataagctaaa tcatctcgaa gataccgaag atgctaaaaa actaaaatcg cagatcaacg
     2221 aaattgcggt tactctaata ggaaaaaccg atgcatctta taaaaccctg agcaaattgc
     2281 ctcgttgatg caaaagattt tagtttccgg agatttcgga aaaatccaga ttctaaaaaa
     2341 gaaaggcact aaaaatattc gcttatcagt gcaccctgtt acaggagtga aaataagcat
     2401 tccctggtat gttagctatg aagaaggtgt ccgattttta catctgaaaa aagactgggt
     2461 actcgcaaag caaacagagt ttaagcagat gcaaaccgaa cggaagggct tcatcggcga
     2521 tggatttgaa tttgcgggct ataagttatt attcaatcat ggcgaccatg caaaaatttc
     2581 tgcccgaatc cacgataaaa atatcgaagt taaactacct atgggaagtg atttgacgca
     2641 aagcttttta cagcaacaac tgcacaaatt ctgcgaaaaa gcactcaaga ttaaagcctc
     2701 tgcaaaatta ccaggcaggg ttgaatatct tgcgaaattg catcggttta actatcattc
     2761 ggttaaaata gggaaaagcc gcactcgttg ggggtcgtgc cgctccgata ataaactcac
     2821 tttttcgtgt tatctgatgc tgcttccaga agaactgata gattttatta taatgcatga
     2881 gctctgccat actgttttta aaaaccacag ccatcagttt cacgcacttg tcaaccagat
     2941 ttgtgataat aatgaagccc tgcttacaaa aaaactcaaa caaataaagc atcccttaat
     3001 gtatgcagtt taactttgct caatgcaaca aaaaaaagta tatatcgaaa cctacggatg
     3061 ccagatgaat gtcgccgaca gcgaattggt agctgcgatg cttcctgatc agaagttcgc
     3121 tatatccgcc agcatcgaaa acgcagatgt tattattatt aatacctgtt cggttcgtga
     3181 acatgctgag aaaaaaattt ggaaccgttt gcagcatatt catggtttaa agcgcaaaaa
     3241 caagcaattg gttaccgggg ttatcggttg tatggcacag cggttaggtg aagaaatatt
     3301 gaatcataaa actgttgact ttgttgcagg tcctgatgcg tatcgtagca tttcgggttt
     3361 gattgaaaag gcacttcaag gtaaaaagga gtcggataca cattttagca tcgacgaaaa
     3421 ttatcataat attctgccaa agcgtagcga tattaatcac atttccggat ttataagtat
     3481 aaccaggggt tgtaataatt tttgcagcta ttgcattgtt ccgtacaccc ggggtcgtga
     3541 acgcagccgc gattatactg atatactttc gcaggccgaa gacctttcta agaatcaata
     3601 tcgtgaaatt tatctgcttg gccagaacgt gaacagttat cagaataaca acctgaattt
     3661 cagcgcactg ttatcgttaa ttgccgatag ctatcctgat atgcgaatac gtttcaccac
     3721 atctcaccca aaagacctca ccgatgagtt aatcgaggtg attgcgaaat ataaaaatat
     3781 ttgcaaccat attcatcttc cggttcagtc gggaagcaat agaattctga aacttatgaa
     3841 tcgcaggtat tcgcgggaat ggtacctgag cagggtcgaa aaaatcaggg aactaatacc
     3901 gggctgtggt ttgagtaccg acattttttg tggtttttgc ggggaaaccg aagacgacca
     3961 tgccgaaacg cttgaactgt tgcgtcgggt tcgtttcgat tctgcgttca tgtttaagta
     4021 ctctgggaga ccgggcactc tggcagcgga gaaaatggca gatgatattc cggaggaatt
     4081 aaaaataaaa agactgaatg aactgatttc gcttcagaat aaaatttctt tacaaagcaa
     4141 taaaaacgat attgggaaac tatttgaagt tctgattgaa aatgaatcga aacgttctaa
     4201 ggattatttc tctgggcgaa ccgaaaccaa taaaactgtt gtattcgaaa aagatcagac
     4261 tcagattggt cagtttgtta cagttatgat taccgatgcc agctcagcaa cattaaaagg
     4321 aaaaattatt aatcatggat aaagttaccg aatttgccaa ttaccgatcg gagatgaata
     4381 aacgtttgct cgaatccgac aataaaatta tcaaacgcat ctttagtctc gacagcatga
     4441 catataccga ttctgctctg tctgcaaaag tgaaagagat gttagggctt gccacatcgc
     4501 tggtgttgcg ttgtgacgat tgcgttaagt atcaccttga gcaatgctac aagcacaagc
     4561 ttagcaccga tgaaatattt gatatacttg cagtttgcaa tgttgtgggc ggaaccattg
     4621 ttattccgca cacccgtcgc gctgtggagt attgggattt actaaataaa taagtacatt
     4681 tacctctgaa taaaatctta cttatgaaaa gccgtttctt ttattcaatt gctttgattc
     4741 tgtttgcgac agtattacta ttttcatgtg gtaaatctgc aaaaaaagca gcacagtata
     4801 atgagaaaat cgtacaggaa caaattaaac tacaagataa ctttaacgct tttgaagcga
     4861 cgtttgccga ttacaaaaaa gaagaaatgt cttcaaagct tcaggaagcc ctgaagcagg
     4921 cagatgcctc gcttgagttt tttaccaaac ttgatgattt cgacaatcaa agtgcatata
     4981 aaaatgctgc cattgaattt gcgtcgatgt ataaaagact ggctactaat gagttcccaa
     5041 aacttgttga tagatattct cttcccgaca gcttattcaa agccgatgtt gaaattgagt
     5101 gtttcaattt actacaagac atagacagca tatacaacga gtcgttcact aaattggtta
     5161 aagcacagaa ggaatttgca agcacttacc aattcaccat tgaagaaaaa aagtgattga
     5221 aggattttgt ccgaatcaga agcttctatt ctatttcata gatagctttt tcatttacgg
     5281 gatatattcc cggagtacct tcatattcct ttaccaaacc tttcacgcag atttttttgt
     5341 tttgaagata tatatgaggc aaatagctga aattgaccag gttcttttta aaaatagata
     5401 ttgaaaaaat ctggtttgga aaactcttat caagattaat aaatacattc ccctttgccg
     5461 atatatgagt gctcaccaca gtgccgcaaa tacacacgtt tttgtttgta cccgaaaaaa
     5521 gagctgcttc aacagtatta tatgtgtttt taggccgttc tgtttctttc aggggtttta
     5581 catcattttt ttgttctacg gttagccaat acttataatc gcattgcgat tcaatactac
     5641 tttcgaggct atcatcaagc gatgcaaaaa agtcgatacc tgtaaaactt tcgagcgagt
     5701 cgattgttat ggcataccac tccacagggt attccaattt agtatgaggc ataagaaatg
     5761 ctattgaacg catattgggt ttatcgaata ctgctttaaa aaagtatgaa ggtaccggta
     5821 gcttgtttac tccctttgta ataaaagtcg cagagtcgga gaaaaccgga cctgtaacaa
     5881 catacacgct tgccgacttc ccttcgatga cataagctct gagtgtattc tcgagttctg
     5941 cccatttttc acggttgaaa tctggaagtt ggggtgaaat attcgaatac agataggttt
     6001 cggaaagtgc tttttgcgac cacctaaaat cggcagaagg aaccaaatgc ccccgatcga
     6061 aaccgaagcc gtcgtatgtt attgtactgt cgggccccac aatgcgtaaa aagtaatcgg
     6121 attcaacagc agtacctcct ttcacaagcg ggtctggccg aaaatcattg gttcggctca
     6181 ccctactttc ggctacttca gaaataatca tatgagccac ccatgctgcc tgatggtgtt
     6241 tttgtgaaaa aaccaatatg taaccgttgt gctcaatcaa ctcctcacct tgcttaattt
     6301 caggtaaacc atacatgttg aggtttgcag caatcattct gaggtttgca tcttcaagca
     6361 gattttccaa actatctgaa attattcgat atctggcaag ctcatgattc agttgcttaa
     6421 ttgtatcttc gacatgttgt gcccaaatat cggcagcaga gcatattacg aaaataatag
     6481 aaatgagtag agtttttact ttttgcatgt ttgattaaag aaatttttta attcacgttt
     6541 tcgttgttcc ggaataccat gccatcgaat gcctaaaata gcaccttcaa ttgcaaaaag
     6601 cgtcgatacg caggcatctg gaaaaacagt tttaagtttt aaaaaaacac cttcagcccc
     6661 catagatttc aaatcggaca ggctttgaaa tcctgcttcg gaaagccttt ctgtaagttt
     6721 aaccccgata ttaggaagtt ttataatttt atccatgaat tcaaaatagt gttatcattt
     6781 gccaatattc tgccgcatta agtcttctat ctcttctgtg actatgggaa tgccatccat
     6841 aagattaact ggctcatccc cgaccagaat atcgttttcg atacgaactc ctattccttc
     6901 cttagcaatg tatatccctg gttcacaggt taaaaccatt cctttgcgga aaacatccga
     6961 ttttgcacca acatcgtgca catcgagacc cataaaatgg gaaactccgt gcataaaata
     7021 ttttttatag gcggcatttt caggtgattg aacagcgaca tctgcaacag agataagccc
     7081 cagcttcagc aactcctcct gcattaattc accggcaatt tcgttcaact ttaaaattgt
     7141 attccccgga acaacatatg atgttatttg tctcgaaacc gataatactg cgttataaag
     7201 actcttttgt ctttcgctga agtacccatt aacaggagca gttcttgagc aatctgcaga
     7261 ataattggcg tattccgcgc caaaatcgag aagtacaaga tctccctcct gcaacacacc
     7321 actgttttta ttatagtgca atatgcatgc gtttgaacca cttgccacga tgggtgggaa
     7381 tgcatggtcc gatgctccgg aacgaataaa ctctgaagtg atggctgcct caatttcata
     7441 ttcgtacttt cccgggcaaa tcgttttcaa tgcctgttga aatgcctctg aagtaatact
     7501 gattgccttt tgtatcagca taagctcttc cggctcctta accagccgaa ggtcagaaag
     7561 aatagggttc aaactgccaa accagcaatc attaaaatga tggcaaattt tactggccat
     7621 cacattatca caggaactat gtatcacagc tccgttttca ttcaccccgt aggggacata
     7681 aatacgggaa gaaaacttaa gtagctctcc gatatatgat tccagctcag gaagcagttt
     7741 tacattggtt atgcctgata tgtcagtaat ttcgttctga ctcatatagt cgccatacca
     7801 tattcggttt ctctcatccg gggccggaac aaacaccgtt tcatgcaagg ctgtattagg
     7861 gtgcgacggg caaagcacca atacggtgtc gtcctgctca atacctgaca agtagaataa
     7921 atcggaattc tggcggtatt tgtaatactg atctcctgaa cggagtttct tctccgatga
     7981 gaaaagcaat attattgctg gggaacaaat tttcccataa agcttcttgc gattccttac
     8041 aaacaaatct gctggtatgg gagaatatct attcatactc cataatctgg atcacaccaa
     8101 tcaccgtttt cttttattaa agatacgagt tcatcaatag ctttgttttc gggaatattt
     8161 ttgcgtaccg ccacacaacc cttatataaa gttacaattc ctttttgcga cccaacgtaa
     8221 ccgtaatcgg aatcgttcat ttctcccggg ccattaacca cacagcccat aacggcaatt
     8281 tttattcctt tcaggtgcga aaatctgatt tttacttgct ttacaattgt ttcgatatcg
     8341 tatgcagttc tgccgcaacc ggggcaagag atgaattctg ccttggtata tctcagccca
     8401 agtgcctgaa acattgcaag tattgcatca aaatgctcat tgtgttgcgc ttcaatcatt
     8461 gggaatatcc cctgggctaa cctgtctaca agtatgctcc cagggtagag gctcatttcg
     8521 gctttggcat tatcgtcata aaacttatta tggtgatatc ttattattag cggtactttc
     8581 aaacccgcat caagcaactg cttataaaaa tagcgaatct ccccggcagg gtactgggta
     8641 attgcattga gtactatact gatccttaat ttagtttgaa caataaactg ctttagtttt
     8701 tgaaattctc ccacgttcat ttccaaaaag aaacgcttac caaccggaat ttttgcttgc
     8761 accagctgag ctattacttc agccgaattg tagtaagggt atatttcatc agtaggatta
     8821 ataccatgta atggggagac gtatttttcg cctgttcctg tcccattgcc agtacaatag
     8881 aatacaaaat ctatttcttc cttgtgctgg gctgataaat acggtaaaac ctctgagtta
     8941 accatgatag cacatttacg tgcattcagt tcttttccct ctcccggtat ttcggtttta
     9001 aaaacttcgt agggagcaat atccataggc atattttgta tccgtggtgc acggtttaca
     9061 taagcagata ccagtttttt tgcaaagcgt atttcgtttt cgggttcttc ggtaagcgat
     9121 acccgtattg tatcaccaat cccatcgttc agcaacgatc cgattccaag tgctgattta
     9181 atccgcccat cttctccttc acctgcctcg gtaaccccta aatgttgagg gaaattccat
     9241 ccgaattcaa gcattcttgc attcagcaaa cgacatgctt tcacggtggt attcacatcg
//
LOCUS       DAOOQP010000118         9103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9430_length_9103_cov_4.440318, whole genome shotgun sequence.
ACCESSION   DAOOQP010000118 DAOOQP010000000
VERSION     DAOOQP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9430_length_9103_cov_4.440318"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLK75_10710"
     CDS             complement(<1..779)
                     /locus_tag="PLK75_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39757.1"
                     /translation="MNRAILLRMFILASMGLFLISPFDGFTQCTNGASTTNVGTLTMQ
                     PVFQTIAVNSGTRRTFTANATITYVFTFCEGGGSYTGDPQLEICDASGATVYAYNDDF
                     CGLGSHISWTCPTTGTYSIVIFRYSCQVTNSPIGTLAYMVLTPPNTQDCLGAIPLCNS
                     FYSTTASYSGTGHYPGEIPTTGGCPGNCMLSGELNDVWYTFTPQSAGFVSFVISPVSS
                     DDYDWAVYDITNYGCEGIYNNVSSTQVSCNWYDGSYYGVYPT"
     gene            complement(825..1376)
                     /locus_tag="PLK75_10715"
     CDS             complement(825..1376)
                     /locus_tag="PLK75_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39758.1"
                     /translation="MKRFSILLLMTFFGLSAFAQENILEFVQKKTTPEGFVIYFDVIG
                     INGDDHANAILEDFMADENVQRGRYFISSANKDRYQLYVNNKVTPNYVLGILKTHGVD
                     FDYTSVSVNGYIKPDDNKSTQNSATSTRTDVQHPGFPKYVDTGNPLQDQQRYADEKSK
                     WINEHPDEYEKMLNGQVAPNEND"
     gene            complement(1389..2675)
                     /locus_tag="PLK75_10720"
     CDS             complement(1389..2675)
                     /locus_tag="PLK75_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY39759.1"
                     /translation="MNKFEQIINERLKNFEAAEIPNAWEDFSKKLPRNAPPPPKGKLG
                     YWLGGAAVLTATIIIIYMAFNDGINANKLTQHKPANKANIVSPSLPTDNSNVQKTAPA
                     LTEKNNLSVPAPDNKNNTVAVPQQVTQKESGSEKLNTTTIDVTEDNVDNKTSASTPET
                     NQINNSDNNASSVDISNITADLNIISQCQPAQVLFSVSNLPESCSVNWITGTEENSNL
                     PETEFQYAEAGTFYPVCMIYNKEGEVVRTFKLSEIMVFPSPNADFTFESKNNLYTFNT
                     LTENVVNYQWELDNQKFSTANLDYRFSKEGNYMVKLIVKNEMGCKSEKSEMISVTINH
                     NYYVPNAFKPFSDGVNSTFGPIGDNLFDYSFKMLIFDKTGKLIFETNNVEHLWNGRIN
                     GTGVMAEPGVYSWEIMTTDKYGNYKHKKGQVTLLMN"
     gene            complement(2662..3249)
                     /locus_tag="PLK75_10725"
     CDS             complement(2662..3249)
                     /locus_tag="PLK75_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY39760.1"
                     /translation="MDLSSRENAERIVNGCVKGDAKCQRELYETLYRRMLGVCLRYSA
                     NNEDAKDLLHDGFIKVFEKLKYFKHSGSLEGWIRKIIVNNAIDNIRRNKNEFLEYEGE
                     HKFERIEDNSDDAIAEMKLTNLKAERLIELIQCLSPAYKAVFNLYVIEEYSHKDIADM
                     LGISVGTSKSNLAKAKLRLKQMYLEKYKSVENEQV"
     gene            3434..3844
                     /gene="mce"
                     /locus_tag="PLK75_10730"
     CDS             3434..3844
                     /gene="mce"
                     /locus_tag="PLK75_10730"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOY39761.1"
                     /translation="MNITHIEHIGIAVKSIDACLPYYEGVLGLKCYAVEVVHDQKVKT
                     AFFHVGQTKIELLEPTDPESTVAKFIEKKGEGIHHIAFAVDDVAASLGQVEAKGVSLI
                     DKTPRGGAEGLTIGFLHPKSTCSVLTELCGHKKE"
     gene            3872..5431
                     /locus_tag="PLK75_10735"
     CDS             3872..5431
                     /locus_tag="PLK75_10735"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOY39762.1"
                     /translation="MAIVNKIGELVSKRAEARLGGGEKRIQAQHDKGKLTARERIELL
                     LDPGSFEEFDMFVTHRCTDFGLEKEIVLSDGVVTGYGTIDGRVVYVFSQDFTVFGGSL
                     SETNARKICKIMDMAMKVGAPVIGLNDSGGARIQEGVQSLAGYAEIFQRNIEASGVIP
                     QLSAIFGPCAGGAVYSPALTDFILMTEDNSYMFVTGPKVTKTVTGEDISVENLGGAKV
                     HAVKSGVTHFTSSDEEEGIKTLRKLLSFLPQNNMETTPLAACTDPVDRVDDILNVIIP
                     DNPNKPYNVIDIINIIVDDGDFLEVHKDYAKNIVVGFARFNGQSVGMVANQPNFLAGV
                     LDVDSSKKGARFVRFCDAFNIPLVTLVDVPGFMPGSAQEYGGIITNGAKLMFAYGEAT
                     VPKVTVTLRKSYGGAHDVMSCKQLKSDINYAWPSAEIAVMGAQGAIEILNFKEVKAID
                     NPEEKTKFIAAKEEEYRYKFANPYQAAKYGFIDDIIEPRNTRFRIIRALASLVTKKDN
                     RPAKKHCNIPL"
     gene            5435..5827
                     /locus_tag="PLK75_10740"
     CDS             5435..5827
                     /locus_tag="PLK75_10740"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOY39763.1"
                     /translation="MTTMWLINAITFSTKAIDDQAWTLFITGITVVFVALWAIAFILR
                     QIQRVIASSESKTLNRKKSKTNTVDVPKKETVVLNSPDDETNVAIALALHFYMDDQHD
                     EESLVLTINTVDRHASPWAAKTFNSLKK"
     gene            5847..6293
                     /locus_tag="PLK75_10745"
     CDS             5847..6293
                     /locus_tag="PLK75_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOY39764.1"
                     /translation="MKEFKFKINGNLFEVSVDTIDESLAEVVVNGKTHRVELLNEIKS
                     PKPKPIVTASNKVKPVEPPDPSSKAGSGPRPSGSAKPILSPLPGIILELKINEGDIIK
                     KGDVVLVMEAMKMENNILAEIDGTVSSIKVSKGQAVMQNDVLVEIA"
     gene            6774..7958
                     /locus_tag="PLK75_10750"
     CDS             6774..7958
                     /locus_tag="PLK75_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOY39765.1"
                     /translation="MKSFWEFTGFANATWGHGVMLLVALFFIFLAIRYHFEPLLLIPI
                     GFGIIIGNIPMFQAVDFNLGLGVYEPGSVMNFIYGGVINGWYPPLIFLGLGAITDFSA
                     LISKPKLMLLGAAAQFGIFAVFMLALLVGFHPSEAGAIGIIGGADGPTSIFTASKLAN
                     GVNVLGYTQDGIPIIAKNLIGPIAIAAYSYMALVPVIQPPIILLLTSKRERKIKMKDP
                     RQVSKLEKILFPIIGVLLTAYLTPTSLPLLGMLFLGNLLKESGVTRRLADSAGNALLD
                     TITILVGVSVGASTQADVFLTSDSILIFAMGAISFMFATAAGVFVGKIMNWLAPKNDP
                     INPMIGAAGVSAVPDSARVVHDMGLKADRTNYLLMHAMGPNVAGVIGSAIAAGILLSF
                     LL"
     gene            complement(7999..>9103)
                     /locus_tag="PLK75_10755"
     CDS             complement(7999..>9103)
                     /locus_tag="PLK75_10755"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOY39766.1"
                     /translation="ISFSGLCQHTHQFHAEFITGTIAPHYSNMKILRQNAVLGGALQY
                     NFIPDKSSSVFELHYKKPEYGAGIEVLSLGNPEMLGKAYSIYLHWGFHAVNKNRSKLK
                     IWFAPGVAYVSKIYDPLTNPENTALSNHLNIYFKLASTYAYQIASAWSIAATVSVTHY
                     SNGATRFPNRGLNQINAGFGVSRNLMSKPVQKTKNRDFPDRGLETWLIGTIGKCDSYS
                     TGINSSGVSYLCNTVTLGLAYRYSWFGKAGLSLDGIINRADHHYWDINWDKMLPVPDQ
                     TFFDYFRLGLCAGHEFTYRNFGFLTYAGLYVYNRVKPHDWSYLRTGFRYYLRPVVLNV
                     TLKSVGFKAQYLEFGAGVYLDGLSIFRKKNKRL"
BASE COUNT         2617 a         1778 c         1886 g         2822 t
ORIGIN      
        1 gtaggataaa cgccataata cgatccatcg taccaattac agctaacctg tgttgatgat
       61 acgttattgt atataccttc gcaaccataa tttgtaatat cgtaaactgc ccagtcataa
      121 tcatctgatg atacaggact gataacaaaa gaaacaaaac ctgctgattg aggtgtgaag
      181 gtataccata catcattcaa ctcgccgcta agcatacaat taccagggca tccacctgtg
      241 gttggtattt ctccgggata gtggcctgtt cctgaatatg atgctgttgt ggaatagaag
      301 ctgttacaca atggtattgc tccgaggcag tcctgtgtgt tcggtggggt tagtaccatg
      361 taggccagtg tgccaatcgg tgaattggtt acctggcagc tgtacctgaa aataacaatg
      421 ctataggtcc ccgtagttgg gcaagtccat gaaatgtggc tgccgagtcc acaaaaatca
      481 tcgttatagg cataaaccgt agctccggat gcatcgcaaa tctccagttg cgggtcaccg
      541 gtataacttc ctccgccttc gcaaaatgta aacacatagg taatggtggc atttgcggta
      601 aatgttcttc gtgttcctga gtttacagca atagtttgga acactggctg catagttaat
      661 gttcctacat ttgtggttga ggccccgttg gtacattgtg taaaaccatc aaatggactt
      721 attaaaaata agcccatcga cgccagaata aacattctca ggagtatagc tctattcata
      781 gttgaaagaa ttacaaattg gttcaatatt ttctcttaaa aaacttaatc attttcattg
      841 ggagcgactt gcccgttcag cattttttcg tattcatcgg gatgttcatt tatccattta
      901 ctcttttcat cagcatatct ttgttgatcc tgtaaaggat tccccgtatc aacatatttg
      961 ggaaaaccag ggtgttgaac atccgttctt gttgatgttg ctgaattttg ggtcgatttg
     1021 ttgtcatcag gtttaatata tccgttaaca gaaaccgaag tataatcaaa atctactcca
     1081 tgtgttttca gaatacccag cacataattg ggtgttactt tattattaac atagagttgg
     1141 tatctgtctt tattggcgga actaataaaa tatctgccgc gctgcacatt ttcatcggcc
     1201 ataaaatctt ctaatatggc atttgcgtga tcatcaccat taatgcctat tacatcaaaa
     1261 tagataacaa aaccttcagg tgttgttttc ttctgaacaa attcgagaat attttcctgt
     1321 gcgaaagctg ataacccgaa aaaggtcatc agtaataaaa tagaaaatct tttcatgtta
     1381 tattgttttt agttcatcaa taaagttact tgtccttttt tatgtttgta attaccatat
     1441 ttgtcggtcg tcattatttc ccaactgtaa acacccggtt cggccatgac accagtaccg
     1501 tttattctgc cgttccatag atgttccaca ttattggttt caaaaatcaa cttgccggtt
     1561 ttatcaaaaa taagcatttt aaatgaataa tcaaataaat tgtcgccaat tgggccaaaa
     1621 gttgagttaa cgccgtcgct gaatggtttg aaagcattag gaacatagta attgtgattg
     1681 atagtaacag aaatcatctc tgatttctca cttttgcaac ccatttcatt ttttacaatc
     1741 aatttcacca tgtagttccc ttcttttgag aaacggtaat cgagattagc tgtgctaaac
     1801 ttttgattat caagctccca ctgatagttg acaacatttt ctgttaaagt gttaaatgta
     1861 tataaattat ttttactttc aaaagtaaaa tcagcatttg gtgaaggaaa aaccattatt
     1921 tcgctgagct tgaaagttct taccacttca ccttctttat tataaatcat acagaccgga
     1981 taaaacgttc ctgcttcggc atattgaaat tctgtttcgg ggaggttgga gttttcttcg
     2041 gtgcctgtaa tccagttcac agagcaagat tccggtagat ttgaaacaga aaacaatacc
     2101 tgagcaggct ggcattgact aataatattt aaatcagccg ttatatttga tatatcaaca
     2161 gatgaagcgt tattgtctga attatttatt tgattggttt ctggtgttga tgcggaagtt
     2221 ttgttgtcaa cattatcctc ggtaacatct atcgttgtgg tatttagttt ttcagaacct
     2281 gattctttct gtgtgacttg ttgaggtact gctacagtat tgtttttatt gtcgggtgcc
     2341 ggaaccgaca aattgttttt ttcggtaagt gccggagctg ttttttgaac attgctatta
     2401 tcagtcggca atgaagggga tactatgttt gctttatttg caggcttatg ttgtgtaagt
     2461 ttatttgcgt ttataccatc gttgaatgcc atgtatataa ttataatggt agcagtaagt
     2521 actgcagcac cacctaacca gtaacccaat ttgcccttag gagggggcgg ggcattccgg
     2581 ggtaattttt tactgaagtc ctcccatgca ttcggaattt ccgctgcttc gaagttcttt
     2641 agtctttcgt tgataatttg ttcaaacttg ttcattttca acacttttgt atttttcaag
     2701 atacatttgc tttaacctga gttttgcctt tgcaaggttc gatttcgatg ttcctacact
     2761 tataccaagc atatcggcaa tgtctttatg agaatattct tcaataacat aaaggttaaa
     2821 cacagcttta taggcgggcg aaaggcactg gatcaattca ataagtcgtt ctgccttcag
     2881 attggtcagt ttcatttctg caattgcatc gtctgaattg tcttcaatcc tttcaaattt
     2941 atgttcgcct tcatattcca gaaattcatt tttatttctg cggatattgt cgatggcgtt
     3001 atttacgatt attttgcgta tccatccttc aagcgaaccg ctgtgtttaa agtatttcag
     3061 tttttcaaag acctttataa atccatcgtg aagcaagtcc tttgcatctt cgttattggc
     3121 agagtaccgg aggcatacac ccaacatcct tctgtataaa gtctcataca gttcgcgttg
     3181 gcatttcgca tctcctttaa cacagccatt tacaatcctt tcagcatttt ccctgctact
     3241 aagatccata attaactttt cgatttattg acgtataaat attattagag ttgtctgaac
     3301 aaattagttt ttttatttaa accgatttaa ttaatgtttt aaataacata taaaacccag
     3361 gttaattttg gcataaaaat aaaaaaaaca aaatcataat tcgttatatt tgcgacttta
     3421 aaaaaaaaat acaatgaata ttacgcacat tgaacacatt ggtattgctg ttaagagtat
     3481 tgatgcatgc ctaccatatt acgagggtgt attgggctta aaatgttatg cagtggaggt
     3541 tgtacacgat cagaaggtga aaactgcttt ttttcatgtt gggcagacta agatagagtt
     3601 acttgaaccc actgatccgg aaagtactgt tgccaaattc attgaaaaaa aaggtgaagg
     3661 catacatcat attgcctttg cagttgatga cgttgcagct agtttagggc aagttgaagc
     3721 aaagggcgtc agccttatcg ataaaacccc ccggggaggc gccgaaggtt tgacaattgg
     3781 ttttctgcat cccaaatcaa cctgtagtgt acttactgaa ctttgtggcc acaaaaagga
     3841 ataaaacagt ttactataat ttatattaca catggcaatt gttaacaaaa ttggagaatt
     3901 ggtttcgaaa cgcgctgaag caaggcttgg gggtggagag aaacgtattc aggctcagca
     3961 tgataaagga aaactaactg cccgcgaacg tattgagctt ttacttgacc cgggcagctt
     4021 cgaagagttt gatatgtttg tgacccaccg ttgcactgat tttggtctgg aaaaagaaat
     4081 tgtgctcagc gatggtgttg ttactgggta tggaactatc gacggcaggg tggtttatgt
     4141 cttctcacaa gattttacag ttttcggcgg ttctctttcc gaaaccaatg cccgtaagat
     4201 atgtaaaatt atggatatgg caatgaaagt tggtgctccc gtgattggat tgaatgattc
     4261 gggaggtgct cgtattcagg aaggtgtaca aagcctagca ggatatgccg aaatcttcca
     4321 gcgtaatatt gaggcatcgg gagttattcc tcagttgtcg gcaatttttg ggccatgcgc
     4381 cggaggtgca gtatattcgc cagcgcttac cgattttatc ctgatgactg aagataacag
     4441 ctatatgttt gttaccggtc caaaagttac caagacggta accggcgagg acataagcgt
     4501 tgaaaatctg ggtggtgcta aagttcatgc agttaagtca ggggtaaccc attttacatc
     4561 aagcgatgag gaagaaggaa ttaaaacact gcgtaaatta ctatcatttt tacctcagaa
     4621 taatatggaa accactccgt tggctgcttg taccgatccg gttgacagag ttgatgatat
     4681 actgaacgta attattccag acaatccgaa caaaccatat aacgtaatcg atatcatcaa
     4741 tataatagtt gatgatggcg atttcctaga agttcataaa gactatgcaa agaacattgt
     4801 ggttgggttt gccagattca atggacaatc ggtgggtatg gttgccaatc agccgaattt
     4861 ccttgctgga gtgctcgatg tggattcatc gaaaaaaggc gcccgctttg ttcgattctg
     4921 cgatgcattt aatattccac tggttactct ggttgatgtt ccgggattta tgcccggctc
     4981 ggctcaagaa tatggaggta ttattaccaa tggcgcaaaa cttatgttcg cctatggtga
     5041 agcaaccgtg ccgaaggtta cggttacctt gcgtaaatcg tatggtggtg ctcatgatgt
     5101 aatgagttgc aaacaactga aaagtgatat caattatgca tggccttctg ctgaaattgc
     5161 agtgatggga gcacaggggg ctattgaaat cctcaacttc aaggaagtta aagccattga
     5221 caatcccgaa gaaaagacaa aatttattgc agctaaggag gaggaatatc gctacaaatt
     5281 tgctaatccg tatcaggctg caaaatacgg ttttatcgat gatattattg aaccacgaaa
     5341 tactcgtttc agaattatca gagccctcgc atcacttgta actaaaaaag acaaccgtcc
     5401 ggcaaagaaa cactgtaata tacctttata aattatgact acaatgtggc taattaacgc
     5461 aataacattt agcactaaag ctattgacga tcaagcctgg actcttttta ttacaggtat
     5521 aactgttgtg tttgttgcgc tatgggcaat tgcctttatt ctcagacaaa tacaaagggt
     5581 aattgccagc agtgaaagca aaacactgaa taggaaaaag tccaagacga atactgtgga
     5641 tgtgccgaaa aaagaaaccg tggtattgaa ttcgcctgat gatgaaacta atgttgctat
     5701 tgctttggct ttgcactttt atatggacga tcagcacgac gaggaatctc tggtacttac
     5761 aattaacact gttgacaggc atgcgtcgcc atgggctgca aaaacattta attcattgaa
     5821 aaaataacag aaatattatc agatacatga aagagtttaa attcaaaatt aacgggaatc
     5881 ttttcgaagt aagtgtcgac acaatcgatg aaagcctcgc tgaagttgtt gttaatggaa
     5941 aaacgcacag ggtggaattg ctgaacgaaa ttaaatcgcc caaaccaaaa cctattgtta
     6001 ctgcttcgaa taaagttaaa ccggttgagc ctcctgatcc ctcttcaaaa gcaggaagcg
     6061 gaccccgtcc aagtggcagt gctaaaccga tcctctcgcc tctaccggga ataattctgg
     6121 agctcaaaat taacgaaggc gatatcatta aaaaaggtga tgtggttttg gtcatggagg
     6181 ccatgaagat ggaaaataac atccttgcag aaatcgacgg aacggtaagt tccataaaag
     6241 tgtcgaaagg acaagccgtg atgcaaaacg atgtattggt tgaaatagct taatttaata
     6301 attttgaaac ttgtaaaatg aagaagtttt tactgctggt tctagcgctt cctttccttt
     6361 ttactgtatc gaaagccgga aatgatgaac ttggtaaagt gctgctgaaa ggcaaatgga
     6421 tgaacgaatc agaaggttac attgcagcgc ctattaagcc ttcaaatacc caaaatgtta
     6481 gggttaccag taggctcaaa tctatccaat ttaatgaaga tggtactttt gaactagact
     6541 cagccaagca aacttatcag ggaagctggt tggtacgaaa tgatactgtg tttttaacat
     6601 tccttccaaa ggcaacagtg cactatgcta aaaaactcgg ttctgcctct acaggctaca
     6661 atatgaccaa cgacacggtg aaactatgcc ccagacatgg taccgtaaac ggtgatgtac
     6721 tttctctaag cgataatcag gaatacactc agcattccgg agctttggta gggatgaaat
     6781 cattctggga atttaccggt tttgccaatg ccacatgggg acacggtgta atgcttctgg
     6841 tagctttatt ttttatattt ctcgctatac gttatcactt tgagccgtta ttgctcattc
     6901 ccatcggttt tggaatcatt attggaaata ttcctatgtt tcaggcggtt gattttaacc
     6961 tcggattggg ggtgtatgaa cccggctcgg taatgaattt tatttatggc ggggttataa
     7021 acgggtggta tccaccgttg attttccttg gactcggtgc aattaccgac ttttcggcat
     7081 tgatttcaaa acctaaatta atgcttttgg gtgctgcagc ccaattcggt atttttgctg
     7141 tatttatgct tgctcttttg gtcggttttc atccttccga agccggagct attggcataa
     7201 taggcggtgc tgacggaccc acttccattt tcactgcatc gaaactggcc aatggtgtta
     7261 atgtgctggg ctacactcag gatggcattc ctattattgc taaaaacctt atcggaccta
     7321 ttgctattgc tgcttattct tacatggctc ttgtgccggt tatacagccc ccgattattt
     7381 tgcttctcac atcgaaaaga gagagaaaaa taaagatgaa agaccccaga caggtttcga
     7441 agctagaaaa aatacttttc cccattatcg gtgttttact aaccgcatac cttacaccaa
     7501 cttcactccc tttgcttgga atgttgtttc tcggcaatct gctaaaagaa agcggggtta
     7561 cccgacgact ggcagactca gccggaaacg ctcttctcga tacaattacc atactcgtgg
     7621 gagtttctgt aggtgcatct actcaggccg acgttttcct tacttccgat tccattctga
     7681 tttttgcaat gggtgcaatt tcgtttatgt ttgctacagc tgccggagtg tttgttggaa
     7741 aaattatgaa ctggctcgca cctaaaaatg atcctataaa tccgatgatt ggtgcggcag
     7801 gagtatcggc tgttcccgac agtgcccgag ttgttcacga tatgggttta aaagccgaca
     7861 gaaccaacta cctcctcatg catgccatgg gtccaaacgt tgccggggtt attggctctg
     7921 caatagcagc cggtattttg ctgagtttct tattgtaatc ggactaatgt ttgattgaac
     7981 gcctgtagag tttatccttt acaggcgttt gttctttttg cggaaaatag aaagcccatc
     8041 aaggtacacc cccgctccaa attccagata ttgcgcttta aaacctactg actttaatgt
     8101 aacattcagt accacaggtc ttaggtagta acggaatccg gttcggagat acgaccagtc
     8161 gtgaggcttc actctgttat aaacatacag gccagcatag gtgagaaatc cgaaatttct
     8221 gtacgtaaat tcatgtcctg cacacagacc taacctgaaa tagtcgaaaa acgtctgatc
     8281 agggacaggc agcattttat cccagtttat gtcccaatag tgatggtcag ccctgttgat
     8341 tattccatcc aacgaaagtc cggctttccc gaaccaactg taacggtatg ccagcccaag
     8401 ggtgacggta ttgcaaaggt agctcactcc cgaactattt attccggtgg aataagaatc
     8461 gcatttgcca atagtgccga taagccaagt ttccaatcct ctgtcgggaa aatccctgtt
     8521 ttttgtcttc tgaaccggtt ttgacattaa attgcgtgaa acaccaaaac ccgcatttat
     8581 ctgattcaaa cccctgttcg ggaaacgggt agcaccattc gaataatgtg ttaccgaaac
     8641 cgttgcagca atgctccatg ctgaggcaat ttgataggca taggttgatg caagtttaaa
     8701 atatatgttc aaatgattac ttaaggcagt attctcaggg tttgttaacg gatcatatat
     8761 ttttgaaaca taagctacgc ccggtgcaaa ccaaattttt aatttactgc gatttttgtt
     8821 gacagcgtgg aatccccagt gcagatatat agaataagcc ttcccaagca tttctgggtt
     8881 tccaagtgaa agaacctcaa taccggcacc gtattccggc tttttatagt gtaattcaaa
     8941 aacactgctg cttttatcgg gtatgaaatt gtattgcaaa gcacccccca acaccgcatt
     9001 ctggcgtaaa attttcatat tgctgtaatg cggtgctatt gtacctgtaa taaactcagc
     9061 atgaaactga tgagtatgct ggcagagtcc ggaaaatgaa atc
//
LOCUS       DAOOQP010000119         9089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9448_length_9089_cov_5.667921, whole genome shotgun sequence.
ACCESSION   DAOOQP010000119 DAOOQP010000000
VERSION     DAOOQP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9448_length_9089_cov_5.667921"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLK75_10760"
     CDS             complement(<1..745)
                     /locus_tag="PLK75_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39767.1"
                     /translation="MILIIWIILSSLSSNAQVADSYCNEIDNPKAVKYFETGVKQLNS
                     GKLDNAIESFMKAIDEEENFTEAWVALSEIYSYRLSNSMDSKNDKMYSTLLKKCILKI
                     AETCPAYDNYSVNYSLGIIFFNDDDYVQAKKFLNIYVKNTEKNTEKYNEAISKLNYIN
                     TYMALIGSPVPFEPRLLGGVCSADDDYLPLISPDGTMAFYTRAYMKKDLNSIYGDQYT
                     EEFTMSQKLSEIDENLVFSRGKAMGNPFNM"
     gene            872..1489
                     /locus_tag="PLK75_10765"
     CDS             872..1489
                     /locus_tag="PLK75_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39768.1"
                     /translation="MKKRRNKTWSQKLDIYHRYNKKIGVYSFLKSNYKKILLVFAVFL
                     AIIVVVNYFYDLETAAEFIVNNYTTDIIFLVFYLSESILGLIPPDIFILWVKGFEDPY
                     LMLGILGVISYLGGLTAYGIGHRLERVPKIREYLEEKYSVHINKIKKWGGIFIIIAAL
                     LPLPYATVLLLAGMLKYPFSRVLYLGIARIARFFIHGVVLFGVIS"
     gene            complement(1484..2356)
                     /locus_tag="PLK75_10770"
     CDS             complement(1484..2356)
                     /locus_tag="PLK75_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOY39769.1"
                     /translation="MSNTKRISKYLSLVKFSHTVFALPFALVAMFSATHQYSYTFDYT
                     ILLLIVLCMVFARNAAMSFNRIIDRNFDAINPRTSNREIPSGKIRLSHAILFCIVNAI
                     LFVVCAGFINTTCLWLSPLALAIITGYSYTKRFTALSHFFLGLALSVAPFGAFVAVSN
                     EIRLFPLLLSMMVLLWTAGFDIIYSLQDISFDRNNQLHSLPAKLGISKSLLISEILHA
                     FVVVAVLLTGLIFSLGMLYWVGSIIFTFMLIWQHIMVNRRKQKNIGIAFGLLNGIASC
                     FYGLFVILDFTLRL"
     gene            2408..2881
                     /locus_tag="PLK75_10775"
     CDS             2408..2881
                     /locus_tag="PLK75_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOY39770.1"
                     /translation="MEEWKKIVTRLQLVPHIEGGYYREIYRSSERVKNSHLAERNLCA
                     SIYYLLCESDFSRFHRIQSDEIWHYYSGNTRLAIYTLQQGQACRIDYLDDESPFLVVK
                     ANTWFSACLETCSNTYVLAGCTTSPAFDYRDFEIADRQQLLYNFPESSVEIVKLT"
     gene            complement(2898..4175)
                     /locus_tag="PLK75_10780"
     CDS             complement(2898..4175)
                     /locus_tag="PLK75_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOY39771.1"
                     /translation="METTPLAERLRPKDFNSFAGQQHLVGKNSSLRLMIESGKIPSMI
                     LWGPPGVGKTTLARIISNYAGRRFYTLSAVSSGVKDVREVIAKAKEQNLFTKGSAVLF
                     IDEIHRFSKSQQDSLLGAVEDGTIVLIGATTENPSFEVITPLLSRCQVYILEYLGETE
                     LKALAEKAIHDPIIGGSRKIKIREHNALFRFSGGDARKLYNIIELIVNSDSSTKIEIT
                     DKLVSEVLQKNISVYDKGGEQHYDVISAFIKTVRGSDPNAAVYWLARMLDGGEDIIFI
                     ARRLVVLAAEDIGLANPNALLMANACFDAVHKIGMPEARIILSECTIYLACSPKSNSA
                     YMAIEKAFEKVRNHGSLPVPLHLRNAPTNLMKQIGYGNDYKYPHDFGQHFTMQQYMPD
                     NLKGDILYNPAENPRENEFKVRLKAFWDGNYKY"
     gene            4234..5034
                     /gene="lgt"
                     /locus_tag="PLK75_10785"
     CDS             4234..5034
                     /gene="lgt"
                     /locus_tag="PLK75_10785"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOY39772.1"
                     /translation="MEPIINFIHWSPDPVIFEIGSRGIRWYGLLLATGFLLSYLVLAN
                     VLKKESWTQQRVDILAIYIIVGVVLGLRLGHCLFYEPEYYLRHPLEILKVWEGGLASH
                     GGAIGILIAVWLFCRKYKYPYLHILDRAAAIVLLAGSFVRVGNLMNSEIYGIPTTVPW
                     AFYFDKARMDGLLPRHPTQLYEAVFYIIAFVVLYKLFMYFKSKWNDGTFLGWFLIVLF
                     VFRFIIEFTKEAQIDNWQHSMKMGQLLSIPFVLLGIFLVVWKGKQKIT"
     gene            complement(5104..6102)
                     /locus_tag="PLK75_10790"
     CDS             complement(5104..6102)
                     /locus_tag="PLK75_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOY39773.1"
                     /translation="MAVSFKELGLVNTSEMFRKAFEGKYAIPAFNFNNMEQMQAIVSA
                     CAETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGWDQPQICLHLDHGDSFEL
                     CKSCVDMGFSSVMIDGSHLSYEDNIALTRKVVEYAHTQNVSVEGELGVLAGIEDEVSA
                     EHHTYTRPEEVVDFVKRTGVDSLAISIGTSHGANKFTPAQCTRDANGMLIPPPLRFDI
                     LTEIEKQIPGFPIVLHGSSSVPPEYVNTINTHGGQLKDAIGIPEDQLRRASKSAVCKI
                     NIDSDGRLAMTATIRKFFTESPEKFDPRQYLGPAREELKKLYIHKIKNVLGSEGKA"
     gene            complement(6291..6797)
                     /locus_tag="PLK75_10795"
     CDS             complement(6291..6797)
                     /locus_tag="PLK75_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39774.1"
                     /translation="MKRFSITILIVIFSASSYFVYAQHHCKANIDSVKQHEWIKQTAS
                     TMYNLKPGDFPKIEPGIQESGDFYMISYKLKSQGSIRLSSGAYIFLMSNSSHENPVVG
                     DITVAIDNKNNIFVNYGHVCGGIINFECNKKILLKSSTDFFDNFTDDTGGTKWVQFNM
                     EVSDPDFE"
     gene            complement(6860..8071)
                     /locus_tag="PLK75_10800"
     CDS             complement(6860..8071)
                     /locus_tag="PLK75_10800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY39775.1"
                     /translation="MTFSQFNFDKRISEAIEAVGYDTPTPVQEKAIPTIMSGRDMIAS
                     AQTGTGKTAAFLLPIIQQLLCIEKNEFIHAVVIVPTRELAVQIGQHITGLAYYTGISS
                     ISVYGGGDGSDFAKEESALQKGTDIVVCTPGRMLSHLSSGKLKLSELKFLVLDEADRM
                     LDMGFYDDILRIVSYLPAKRQTLMFSATMPVNIRRIARQILKNPYELNIAIATPPEKI
                     EQCAYMIPNSAKKTLIRIILQEKHYRSVLIFCSTKSGVKELTKVLHQAGLNASEIHSD
                     LEQSKREEVLLAFRNRKIETLVATDVLSRGIDVDDIELVVNYDVPSDAEDYVHRIGRT
                     ARAESTGIAITLVGEKDRFAFSKIEKLIGKKINIREVPDVLGISAVTTDGHSRKSKHR
                     FGKQKHIKSRS"
     gene            complement(8165..8500)
                     /locus_tag="PLK75_10805"
     CDS             complement(8165..8500)
                     /locus_tag="PLK75_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39776.1"
                     /translation="MKKLILTLFVLASLPFLIFAHPPKKVKLNYTPGELVIEVIHPVK
                     DAAQHYIDQITILIDGKEYKILKYSSQTTNESLIIEVKVPDLQKGSVIQVKANCNKMG
                     LKSGKLKVK"
BASE COUNT         2642 a         1740 c         1790 g         2917 t
ORIGIN      
        1 ccatattaaa aggattaccc attgcttttc cgcgcgaaaa aacaaggttt tcatcaattt
       61 cgctaagttt ttgcgacatc gtgaattctt cggtgtactg gtctccgtat atactgttta
      121 aatccttttt catgtaggcg cgcgtataaa aagccattgt gccgtcggga gaaatcaaag
      181 gtaaatagtc gtcgtcagct gaacaaaccc cgccaagtag cctgggctcg aaagggacag
      241 ggctcccaat caaagccata tatgtgttga tgtaattcaa tttcgaaatt gcttcgttgt
      301 atttttctgt atttttttct gtgtttttaa cataaatatt caggaatttt tttgcctgaa
      361 cataatcgtc atcgttaaaa aaaataattc caagcgaata gttgaccgaa tagttatcgt
      421 atgccggaca tgtttcagca atttttaata tgcacttctt cagcagggta gagtacattt
      481 tgtcgttttt agagtccatt gaattcgaca atcggtaact gtatatttcg gaaagagcaa
      541 cccaggcttc cgtaaagttt tcttcctcat caattgcttt cataaagctt tcgatggcat
      601 tgtcgagctt gccggagttc aattgcttaa ctccggtttc gaaatactta acagcctttg
      661 ggttatcaat ttcgttgcaa taagagtctg caacctgtgc attgcttgat aacgaggaca
      721 ggatgatcca aattattaga atcaaatatt gtaattgtaa tttcatttaa tagaaacgct
      781 attacaaaga taaaattttg cgcaaaccca cgaattgata attatcaata aatgtgcctt
      841 cgattttcaa aaaattataa atttgtagat gatgaaaaaa cgtagaaata aaacttggtc
      901 gcagaagctg gatatatatc accgttacaa caaaaaaatt ggtgtgtata gcttccttaa
      961 aagtaattat aagaaaatat tactggtctt tgccgttttt ttagcgatta ttgttgtagt
     1021 gaactacttc tacgatctgg aaacggctgc tgaattcatt gttaacaact atactacgga
     1081 tataattttt ttagtttttt atttatcaga gtcaatatta gggcttattc cacccgatat
     1141 ttttatttta tgggtgaaag ggttcgaaga tccctatctg atgttaggta ttttgggggt
     1201 gatttcgtac ctcggtggtt taaccgctta cggtataggg cacaggcttg aacgagtacc
     1261 taaaatcaga gaataccttg aagagaaata ttctgtgcat ataaataaaa ttaaaaaatg
     1321 gggtgggatt tttattatta ttgcagcact tctgccattg ccctatgcaa cagtattact
     1381 ccttgcaggt atgcttaaat atcccttttc aagggtcctt tatttgggta ttgcccgaat
     1441 agcacggttt ttcattcacg gtgttgtcct ttttggagta atatcataac cgcagcgtaa
     1501 aatcaaggat aacaaacaga ccgtaaaagc acgatgcaat gccgttaaga aggccgaaag
     1561 caatgccgat gtttttctgc ttcctgcgat taaccatgat atgttgccat ataagcatga
     1621 atgtaaaaat aattgaacct acccaatata acattcccag tgaaaatatt aaacctgtta
     1681 aaagaactgc tacaactaca aaagcatgaa gaatttctga aattaacagt gatttactaa
     1741 tgccaagttt tgcaggtaag gaatgtagtt ggttgttacg gtcaaacgaa atgtcctgca
     1801 gcgaataaat aatatcgaaa ccggcagtcc acaacagcac catcatcgac aataacaacg
     1861 gaaacaggcg aatctcgttg ctcacagcta caaatgcccc gaatggcgca accgacagtg
     1921 ccagcccgag aaaaaagtga ctcaaggcag tgaatctttt cgtatagctg tatccggtaa
     1981 ttattgccag agccaatggc gagagccaca ggcaggttgt gtttataaaa ccagcacaaa
     2041 ctacgaaaag aatcgcattt acaatacaga ataatatggc atgactcaag cgtatttttc
     2101 ctgatggaat ttccctgtta ctggtgcgag gatttatagc atcgaaattc ctgtcgatga
     2161 tacggttaaa actcatagct gcatttctgg caaataccat acagagaaca atgagcagca
     2221 atatggtata atcaaatgta taggagtatt gatgcgttgc cgagaacatt gcaaccaaag
     2281 caaaaggcaa tgcaaatacc gtgtggctga attttaccag tgataaatac ttgcttatgc
     2341 gttttgtgtt gctcaatttt gtagttttaa caaaattaat gaaacagaac gaaacttttg
     2401 atgttttatg gaagaatgga aaaaaattgt cactaggcta cagcttgtac cccatattga
     2461 gggtggttat tacagggaaa tatacaggtc gtctgaacgc gtcaaaaaca gccaccttgc
     2521 tgaacgcaac ctgtgtgcaa gtatatatta tctgctatgt gaaagcgact tcagcagatt
     2581 tcatagaata cagagcgacg aaatatggca ctattactcc ggcaatacac gccttgctat
     2641 ttataccctg cagcagggtc aagcttgccg catcgactac cttgatgatg aatccccatt
     2701 tcttgttgtc aaagcaaata catggttttc cgcctgcctc gaaacctgca gcaataccta
     2761 cgttctagct ggttgcacca ccagcccggc atttgattac cgggattttg aaattgcaga
     2821 ccggcagcaa ctgctttaca attttcccga atcatctgta gaaattgtta agcttacctg
     2881 atgtaggtga tatattttta atacttgtaa ttaccatccc agaatgcttt caagcgcact
     2941 ttgaattcat tttctcttgg gttttcggca gggttatata aaatatctcc tttaaggtta
     3001 tctggcatat attgttgcat tgtaaagtgc tgtccgaaat catgagggta tttgtaatca
     3061 ttcccatagc ctatctgttt cattaaattt gtaggcgcat ttcgcaaatg caggggtact
     3121 ggcagtgaac cgtgattccg aactttctca aaagcttttt cgattgccat atatgccgag
     3181 ttgctcttgg gtgagcaggc aagataaatg gtacattccg aaagaataat tctggcttcg
     3241 ggcattccaa ttttatgcac ggcatcgaaa caagcatttg ccataagcag ggcgttgggg
     3301 ttggcaagtc cgatatcttc agcggccagt acaaccagcc ttcgtgcaat aaaaatgatg
     3361 tcttcacctc cgtccaacat acgcgcaagc caatacacag cagcattagg gtctgaaccc
     3421 ctaacggttt ttataaatgc cgaaattaca tcgtagtgct gctctccgcc tttgtcataa
     3481 accgaaatgt ttttctgaag aacctctgaa accagcttgt cagttatttc aattttcgtt
     3541 gagctgtcgg agttaacaat cagttcgatg atgttgtaaa gttttcgcgc atcgccgccc
     3601 gaaaaacgaa agagcgcatt atgctccctg atttttattt tacggctgcc tccaattata
     3661 ggatcatgaa tggctttttc ggcaagtgct ttaagctctg tttcgcctaa atactccaga
     3721 atatatacct gacaacgtga aagtaagggt gttattactt caaatgaagg gttttcggta
     3781 gttgcaccaa taagtacaat tgtaccatcc tcaacggcac ccagaagaga atcctgctgc
     3841 gatttgctga agcggtgaat ttcatcgata aataatactg ccgaaccctt tgtaaaaagg
     3901 ttttgttctt ttgctttggc tatcacctcg cgcacatcct tcactccgct gctcacagcc
     3961 gagagagtat agaaacgcct tcctgcataa ttcgaaataa tccgggctag agttgtcttt
     4021 ccaaccccgg gcgggcccca caatatcatt gaaggtattt ttccggattc aatcatcagg
     4081 cgcaggcttg agttttttcc aacaagatgc tgctgtcctg cgaatgagtt aaaatctttg
     4141 ggacgtagcc gctcggccag tggtgtcgtt tccataatta ttttgtattc ttgtttttgt
     4201 cggtatattt gctggctaaa ttaatgaatt tacatggaac caataattaa ttttattcac
     4261 tggagtccgg acccggtgat ttttgaaatc ggtagccgtg gaatccgttg gtatgggttg
     4321 ttgctggcta ccggattttt actttcgtat ttggtgttgg ccaatgtatt gaaaaaggag
     4381 tcgtggacac agcagcgcgt cgacatcctg gctatttata ttattgtggg cgtagtatta
     4441 gggctacgcc ttgggcattg tttgttctac gaaccagaat attatttacg ccatccactg
     4501 gaaatattga aagtgtggga gggcggactt gccagtcatg gcggtgctat agggattctc
     4561 attgctgtct ggcttttttg ccgaaagtat aagtaccctt atctgcatat tctcgacagg
     4621 gctgcggcta ttgtgctcct tgccggaagt tttgtcaggg ttggaaacct gatgaactcg
     4681 gaaatctatg gaattcccac tactgttcca tgggctttct acttcgataa agctcgtatg
     4741 gatggtttgc ttccgcgcca cccgactcag ttatacgagg ctgtttttta cattattgcc
     4801 tttgtggtat tatataagct gtttatgtat ttcaaatcga aatggaatga tggtactttt
     4861 ctgggttggt ttctcattgt gctgtttgta ttccgcttta ttattgagtt taccaaagaa
     4921 gcccaaatcg ataactggca gcattccatg aaaatggggc agcttttaag cattccattt
     4981 gtgttgctgg gtatttttct tgttgtttgg aaagggaagc aaaaaataac ctgatgggtt
     5041 gaaaaataaa agcacagaca aaatggttgt ttttctgtgc ttcataaatt tgtatgtaaa
     5101 ttactaggct tttccttcgc ttccaagtac atttttaatc ttatgaatat aaagcttttt
     5161 tagctcttcg cgggcaggtc ccaagtactg acgcgggtcg aacttttcag ggctttcagt
     5221 aaaaaatttg cggatggtag ctgtcatggc caaccttcca tcggagtcga tgttgatttt
     5281 acaaacagct gattttgatg ccctacgcaa ttggtcttca ggaattccta tggcgtcttt
     5341 cagttgacct ccatgagtgt taattgtgtt tacatattca ggtggaaccg atgatgaacc
     5401 atgcaataca atgggaaatc cgggtatttg tttctcaatt tcggttagta tgtcaaaccg
     5461 caatggtgga ggaatcagca ttccgtttgc atcgcgagta cactgggctg gcgtgaattt
     5521 attcgcgcca tgagatgtgc ctatcgaaat agcgagcgaa tcaacgccgg tccgctttac
     5581 aaaatcaacg acttcttccg gacgtgtata ggtgtgatgt tctgccgaca cctcatcttc
     5641 gatgcctgca agtacgccga gttcaccttc taccgaaaca ttttgggtat gcgcatattc
     5701 tactactttc cgtgtaagtg cgatattgtc ttcatacgac aaatgtgaac cgtcgatcat
     5761 taccgatgag aaccccatat ctacacaact tttgcataat tcaaagctgt cgccatggtc
     5821 gaggtgtaag caaatctgtg gttgatccca tccgagctcc ttagcatatt ccacagcacc
     5881 ctgagccata tatcgcagca atgtctggtt cgcatatttg cgtgctccgc tggaaacctg
     5941 taaaatcaca ggggattttg tttcggcaca tgccgaaaca attgcttgca tctgctccat
     6001 attattgaaa ttgaatgccg gaatagcata tttcccttca aatgctttac gaaacatttc
     6061 tgaggtattt accaacccca gttctttaaa acttactgcc atgtcgaaat aattttataa
     6121 ttaaacaagt ttttaacatc ataatttcct gctaaattac acaatgctat ctgcattaac
     6181 aatgataatg gcttaataat tacatattaa gaaacatatt acgcgaaatt ggaagttgat
     6241 ttgatagtta atatttgagg aacaaccagg attgccctgg ttatctgtgt ttattcaaaa
     6301 tcgggatcag agacttccat attgaactga acccacttcg ttcctccggt atcatcggtg
     6361 aaattatcga aaaaatcggt agagctttta agcagaattt ttttattaca ttcaaaatta
     6421 ataattccac cgcacacatg cccatagttt acaaagatat tattcttatt gtcgatagcc
     6481 actgttatat ctccgacaac cggattttcg tgacttgagt tagacatcag gaaaatataa
     6541 gcgccactag atagccttat tgaaccttgc gatttcaatt tgtaggagat catgtaaaag
     6601 tcacccgatt cctgaatacc cggttctatt ttagggaaat cgcctggttt taagttatac
     6661 atggtcgatg cagtttgctt aatccattcg tgctgtttca ctgaatcgat attagctttg
     6721 caatgatgtt gagcatagac aaagtagctg gatgctgaaa aaataactat taaaatggtt
     6781 atgctaaatc ttttcatatt tattgctcgg ttttacaact gtaaatatac cattttattt
     6841 gactgtggtg tgttgaggtt taggatcggc tttttatatg tttctgtttg ccaaatctat
     6901 gcttggactt ccgtgaatgt ccatcggttg tgacagcaga tatacccaga acgtcgggca
     6961 cttccctaat gttgattttt ttgccgatta gtttttcgat tttcgaaaat gcaaaacggt
     7021 ctttttcgcc cacaagagta attgcaatac ctgtgctttc ggctctggca gtacgaccaa
     7081 tgcggtgcac ataatcttcg gcgtcagaag gaacatcgta gtttactacc aattcaatgt
     7141 cgtcaacatc aatacctctc gataatacgt cagtggcaac cagtgtttca atttttcggt
     7201 tcctgaaagc aagcaacacc tcttcgcgtt tcgattgctc aaggtcagaa tgtatttccg
     7261 atgcatttaa tcctgcttgg tgaaggactt tggttagctc cttaactccg ctttttgtag
     7321 aacagaaaat aagtacactt ctatagtgtt tttcctgcaa aataattcgg ataagcgttt
     7381 tttttgctga attgggaatc atataggcac attgctctat tttttcggga ggagtagcaa
     7441 tagcaatgtt caattcataa ggattcttaa ggatttgtct ggcaattcga cgaatattta
     7501 ccggcatagt tgccgaaaac attaaggtct gacgctttgc aggcaaataa gagacaatac
     7561 gcaggatatc gtcgtaaaat cccatatcga gcattctgtc agcctcgtcg agtaccaaaa
     7621 attttaactc cgacaattta agctttccgg aactcaggtg tgatagcatt ctgcctggag
     7681 tacaaacaac tatgtcggta cctttttgca atgcactttc ttctttggcg aaatcagaac
     7741 catctccgcc gccgtacacc gaaatagaac taattcctgt gtaataggcc agccctgtaa
     7801 tatgttgccc gatctgtacc gccagttcac gcgttggtac tattaccaca gcatgtatga
     7861 actcgttttt ttcgatacag agcagttgtt gaataatagg cagcaaaaag gcagctgttt
     7921 taccggtacc tgtttgtgct gaagcaatca tgtcgcgccc gctcatgatt gtagggatag
     7981 ctttttcctg aacaggagtg ggtgtgtcgt agccaacagc ttctatggct tcagaaatac
     8041 gtttatcaaa attgaattgc gaaaatgtca taatgtaaat taaaaaaagc cggagctata
     8101 tcggcataat tccggcaaaa ttataaagtt tcagttaaat aactctataa taaagataaa
     8161 tctgttattt gactttgagc ttacctgatt ttaaacccat tttattgcaa ttagccttta
     8221 cctgaataac actgcctttt tgcaagtcag ggaccttcac ttctattatc aggctttcgt
     8281 tagtagtctg actggagtat ttaagaattt tatattcttt tccatcaatc agaatggtaa
     8341 tttgatcgat gtagtgttgc gctgcatctt taacaggatg aataacttca attaccagtt
     8401 ccccgggtgt gtagttcagt tttacttttt tcggaggatg cgcgaaaatt aaaaatggta
     8461 acgatgccag aacaaataga gtcaggatta attttttcat aattttcggt tttaaatttg
     8521 tataaaaaca attcagaaag tagtttgttc aaaattaatt taaaaaatgg aattagcaaa
     8581 gaaaaatcaa gattctcttg aacaacttta acattaattt agattgaata aaaattatta
     8641 ttttcataaa gtggtgaaaa tcaattcgta aaacaatcat ggtttatttt agtaatgtta
     8701 aatactttct aatttcatta agtaaaaata tccgattaaa tagctgttat ttataaactg
     8761 atttatactg aatttgcagc attttttaca aattgcatat agttgaaaaa tagtttgtta
     8821 tcgtaatgct ataatattat atattatatt catttaagct gatgaaaagt gtaaaaaaca
     8881 cagtattgtg atttgtataa aaccatttgc aaaacgaaaa atcatttatt aaatttatag
     8941 agttgttttc agcggaaatc gattaattac taaatttaaa tatttgaaaa acttctacac
     9001 aagcacttct agacaaaatt cttttcgatg caaaaatcac tattgtccga taaaaatatg
     9061 gaataatttt taatttttgc taagttatt
//
LOCUS       DAOOQP010000120         8908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9703_length_8908_cov_5.147634, whole genome shotgun sequence.
ACCESSION   DAOOQP010000120 DAOOQP010000000
VERSION     DAOOQP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9703_length_8908_cov_5.147634"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLK75_10810"
     CDS             complement(<1..284)
                     /locus_tag="PLK75_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39777.1"
                     /translation="MKNIYFLICFLLFTPLAFSQAKSGRLVIVHTNDMHSMLTGFSPE
                     MEYTPQTTGDDFTLGGFSRISAFIKEIQKQHPENCIVMDAGDFLMGSFFH"
     gene            complement(423..1103)
                     /locus_tag="PLK75_10815"
     CDS             complement(423..1103)
                     /locus_tag="PLK75_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39778.1"
                     /translation="MKYWMFVLMLVFASVCFGQSLSLEKSHFKPGETIKLSFTALSSY
                     ESNAWIGIVPSSIAHGSEDVNDQHDITYQYLNGKTSGVLSFTAPAAEGNYDFRLHNSD
                     NNGKEVAYFSFTVSTTDFVATETFRNASMHLEKLVFSPGEKINLYYTAPPFTGSNPWI
                     GIVPSDKPHGSETQNDQYDISYQYIGSKTSGTLSFTAPTTPGNYSFRMHDTDANGKEV
                     SNISFTVK"
     gene            1266..2393
                     /locus_tag="PLK75_10820"
     CDS             1266..2393
                     /locus_tag="PLK75_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY39779.1"
                     /translation="MQSRPNTSLISYFSNMVKQSGGINLAQGLPGFRPPLKLMQNLSE
                     ISLTDSHKYAPGLGLDLLRCQIAESCNSTAIGAENVMITNGGTEAISLIYTWLYKKLH
                     HQLNVMSFAPVYESYRELPGIFGNHFYAFRNDPNQSINIPLLCEELARNEINLVFLAT
                     PGNPLGRIFSEYELTEICNYCSRKRIYLVIDLVYKYLYYDKKPSIPYSALSEYILFAD
                     SFSKQFSITGWRLGYLIAPPEIICEIASVHDYTGLSSPLPLQKALSMYLSNTSDALQY
                     LQDTREKVSENFEYASHRLSEAGFRCIPAHGGIFLCTALPDHFDDGFQFASNLYKSCK
                     VAVVPGEHFDREWKRIIRLNIAHPADVLNQALDEIIHFSAS"
     gene            2450..2533
                     /locus_tag="PLK75_10825"
     tRNA            2450..2533
                     /locus_tag="PLK75_10825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2484..2486,aa:Leu,seq:gag)
     gene            2626..3717
                     /locus_tag="PLK75_10830"
     CDS             2626..3717
                     /locus_tag="PLK75_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOY39780.1"
                     /translation="MIKIRSLILCFFSVIFLFTGKVFSQTDSLFLQPTTVLSAEQIAA
                     HDSVENIRRQLIDSIITYGKTYVGLHYRYGGITPSGFDCSGFVFHVHRHFNLPIPRVP
                     SATCLMGERIELDSLQPGDLVYFKGRHTNSNTIGHVAMVVEKLPGDFIMIHSASHAGV
                     IVETFADKSYYTSRYLFANRLPTEFYLRQWNDSLMKIYSYRNADIYKEYAATPVTVAQ
                     PEGTTLLNYTVKSGDMLGLIASWYKVTVNELMAWNGLSSTRLSIGQNIKVYVKTDQLS
                     KFSNMNTMSFDEKQKSSGSSSSNKTVTGAGTREDASYEYYTVQSGDSPYSIAKKFPGT
                     TAESIMKLNNISNASGLQIGQKLKIRKKN"
     gene            3833..4753
                     /locus_tag="PLK75_10835"
     CDS             3833..4753
                     /locus_tag="PLK75_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOY39781.1"
                     /translation="MSGQSSSKKSILFAFAANIGIAITKTVAAVITGSGAMLAEAIHS
                     YADSGNQVLLFAGLRAARKKPTQTYPLGYGKSVFFWSFVVALILFSVGGLFSVYEGIH
                     KLNSHNGVSSPLIAIVVLSISILLEAASLYGCITQIRKVKRKNENLLQWFKHSRQSEL
                     VVVLGEDVAALLGLLFALIAVLLTMLTGNPVYDAAGSIGIGALLIIVSIFLAVKIKGL
                     LIGQSADEKTVLEIKEFIESRHEVDTLMNIITFQLGPDIMVAVKARMKETQSASVLID
                     HINLCEREIKKNFPDVKWMFFEPDIADSKN"
     gene            complement(4750..5274)
                     /locus_tag="PLK75_10840"
     CDS             complement(4750..5274)
                     /locus_tag="PLK75_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39782.1"
                     /translation="MNAPVLSRRTRKLLTIALIFVMGYCLVALVEKYPNIWIVYIVLA
                     AVSLAAFLIIRFIMNVFGYYTTKILKFKNRYSSWEKSEAVVFHKRGPEFFGKLHCTIV
                     SVIYCDQNESIVCASVIYDDLNKKNDLNSLTHFDAVSHLKIDRKVQIKYNPSDPRQIM
                     IPVVHMIDLGLKGY"
     gene            complement(5386..8244)
                     /locus_tag="PLK75_10845"
     CDS             complement(5386..8244)
                     /locus_tag="PLK75_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOY39783.1"
                     /translation="MDNNDKTKIYKDLQNQKTGLYNAQSQNGELLKGNMQPAESSLIP
                     GEKISLRNKEYNVLSTLSEGTGEAIVYLIRDSNSKDFALKLYYDYLQGAEEPNGIALE
                     RIKSLNDEDILKLYDFGVGADKFRGKNCYEISQYARGGNLLDVPNFKQKYTALFLENQ
                     LIPQIFKAITKLHEYKIYHCDLKPQNILYLDETQNDLVIGDYGSAKAWDLQSEMELRR
                     SKIVKGTEAYLAPEQARGITSEKNDYYSFGMILLHLLYPEQICTSENFSEIDKEKFSI
                     LIEKQYNGGPLVKFSPEYERLNRLITGLTMAYYHDRWGWSEVEAWLKGKFLDAEAGRK
                     QLVTDENLLIVDGIEIHNVHELALEIENRSDFIDVLLEDEDNFSALKTWLIQTKDIAY
                     KNKLVNIIAFHREMGKKYLKDALNLFLGESYVVNYEGVTLNLAKSNDLSHEINTLRKD
                     MAEWNNSEKELAVFFRVEFILRLIAESPTAKNNKTSTAILHSLIKEFKIDSADVQRIA
                     CEITKARLIQTQHAVQEFESVGKTQQNKGFQKQNKENNKTLGIKKQADADRSFQQKIN
                     IFESEANGIRFYGHYSTEAAMNYAKAWYFYFFYTEAESRRFNQVETMAAIKKIIVDNG
                     WNEKEMNHYAIYSHNSKATRMQLESHGLQAEGFNTQSAWSYVDLEKRFVFYSENGFNV
                     PVNAENIALIQRKNMLLKLFVVYPLMDDSRLPKKKAQPKLSRSEHVTKLNYPLLCTSK
                     NKYADSFFSGEFDTVLNFVDTHPSRPIECEKVKELGRFTVSDALLQYFLYLKYNRKDG
                     IREINNGFSMEAYCNKINNILSQDKSNSTEITIENIRMAIDEEYEKIKVWCQDWCKKN
                     SWIVWYPKKNYRKTGIKMIDFVTLYNGNLYSAYRCVTTNKLLEAFNDLLEAYNKIDTM
                     LYFGIYDIENKWWYYFGDTFVTDYAAYYTEMKYKYDKI"
     gene            8683..8898
                     /locus_tag="PLK75_10850"
     CDS             8683..8898
                     /locus_tag="PLK75_10850"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOY39784.1"
                     /translation="MKNITVKHVILAISVLLLLIFTFQNLETVRVHFLFFDFGMPLVI
                     LIVLVFFIGFFTAWVMKKLKKKNIDLR"
BASE COUNT         2598 a         1757 c         1660 g         2893 t
ORIGIN      
        1 atatgaaaaa aactgcccat cagaaaatcg ccggcatcca taacaatgca attttcggga
       61 tgttgcttct ggatttcttt tataaaagcc gatatcctgg aaaaaccacc cagagtaaaa
      121 tcatcgccag tagtctgagg ggtgtactcc atttcgggcg aaaaacctgt gagcatgctg
      181 tgcatatcgt tagtgtgcac aatcaccagc ctacccgact ttgcctgact aaaagccagc
      241 ggggtaaaaa gcaaaaaaca aattaaaaaa tagatgtttt tcataaactg catttattgg
      301 gtactaaagt aataatttat ttttcagcag tatgtaataa tttacacaaa gagttacatt
      361 tataaaacct ttaattcgtt gattacaaat tatctgcttt caatgaagtt taaattgttg
      421 gactacttta cagtaaatga tatattactg acttcttttc cattagcgtc ggtatcgtgc
      481 attctgaagc tatagttacc gggagttgtg ggggcagtaa agcttaatgt tccggaggtc
      541 ttggatccaa tgtattgata actgatgtcg tactgatcgt tttgcgtctc agagccatgg
      601 ggtttatcgg aagggactat gcctatccac gggttactgc ctgtgaaagg gggtgcagta
      661 tagtaaagat tgattttctc tccgggtgaa aaaaccagtt tttcaagatg cattgaagca
      721 tttcgaaatg tttcagtagc gacaaaatct gtagtgctta cagtaaaact gaaataggcg
      781 acttcttttc cattgttatc gctattgtgc aatctgaaat cgtaattacc ctccgcagct
      841 ggtgctgtaa agctgagaac tcctgaagtt tttccattca ggtactgata tgtaatgtcg
      901 tgttgatcgt tcacatcttc actaccgtgc gcaattgacg aggggactat tccaatccat
      961 gcattggatt catagctcga aagggctgta aaacttagtt taattgtttc accgggtttg
     1021 aaatgcgatt tttcaaggct caggctttgt ccaaaacata cactcgcaaa aactaacatc
     1081 agaacgaaca tccagtattt cataattgtt taattttaag tttttcaaat ataccgtttt
     1141 atagttatcc aaaattcact aagccgtgat tctgcgatat ttttacataa aaagcgaatc
     1201 acggatgcat caacatacat gcataaaaca aatcaatcgt atatttgaac caaatttacc
     1261 cgtttatgca atcacgtccg aacacttctc tcatcagtta tttcagcaat atggtaaaac
     1321 aaagcggagg tattaatctg gcgcagggtc tgcccggttt ccggcctccg ttaaagctga
     1381 tgcaaaatct ttcggaaatt tcattaaccg attcccataa gtatgctccg ggcctggggc
     1441 ttgatttatt acgttgtcag atcgcagaga gttgcaactc cacggcaata ggtgcagaaa
     1501 atgtgatgat aaccaatggc ggcactgaag ccatttcgct tatatataca tggctttata
     1561 aaaagctaca tcaccaactc aacgtgatgt cgtttgcacc cgtatacgaa agctaccggg
     1621 aattacccgg catatttgga aaccatttct atgcttttcg taatgacccg aatcaaagca
     1681 ttaacattcc attgttatgt gaggagttgg cccgaaatga aatcaatctt gtttttcttg
     1741 ccactcccgg aaatccgctg gggcgcattt tttcggaata cgagcttacc gaaatatgca
     1801 attattgttc ccggaaaagg atttaccttg tgattgacct tgtgtacaaa tatctgtatt
     1861 atgataaaaa acccagcatt ccttactctg ctctatcgga gtatattctg tttgccgaca
     1921 gtttttcaaa acaattttca attaccggat ggcgcttggg gtaccttatt gcaccaccag
     1981 aaattatatg cgaaatagca agcgttcacg attatacagg tttatcgtcg ccattaccac
     2041 tccagaaggc actcagcatg tatctgagca acacatcgga tgctttgcaa tacttacaag
     2101 atacaagaga aaaagtatcc gaaaatttcg agtatgcttc tcataggctg agtgaggccg
     2161 ggttccggtg cattccggcc catggaggga ttttcctttg cacggccctg cccgaccatt
     2221 tcgatgatgg gttccaattt gccagcaatc tgtataagag ttgtaaagta gccgtagtac
     2281 ctggagagca tttcgacaga gaatggaaaa ggatcatcag gttgaatatt gcccacccgg
     2341 cggatgtact caatcaggca cttgatgaga tcattcactt tagcgccagc taaaaaaaca
     2401 tggagcttgt tgcagtaata aaaaaattat tatttttgca ttcacatttg cccagatggt
     2461 gaaattggta gacacgccag cttgaggggc tggtgtcctt aaggatgtgc aagttcgagt
     2521 cttgttctgg gcactgaaaa cgaagcagtt ttactgtttc gttttttttt gagtaacaat
     2581 taggtaagtt tgcctgtttg gttttctttt gtatttttgc cggtaatgat aaaaatacga
     2641 tcattaatac tttgtttttt ctcagtgatt tttcttttta caggaaaagt attttcacaa
     2701 accgatagtc tttttcttca accaacaacg gtacttagtg ctgaacaaat tgctgcacat
     2761 gactctgttg aaaatatacg gcggcaattg attgattcaa tcattacata tggaaaaacc
     2821 tatgtaggat tgcactaccg ctatggtggt attactcctt ctggtttcga ctgcagtggt
     2881 tttgtttttc acgttcacag gcatttcaac ctgccaattc cccgtgtacc cagtgctacc
     2941 tgtcttatgg gagaacgcat tgaacttgat agcctacaac caggggatct tgtatatttt
     3001 aaaggacgcc acaccaacag caatacaatc gggcatgttg ccatggtagt tgaaaaactt
     3061 cccggtgact ttataatgat tcactcagca agccatgcag gggttattgt tgaaactttt
     3121 gcagacaaat catattacac ttcccgctat ttatttgcaa accgcttacc cactgagttt
     3181 taccttaggc aatggaacga cagtctgatg aaaatctaca gctaccgtaa tgccgacatc
     3241 tataaagaat acgctgcgac tcctgttacg gtagcccaac ctgaggggac aaccctactg
     3301 aattatactg taaaatcggg tgatatgctt ggactaattg cttcgtggta taaggtaact
     3361 gtaaatgaat taatggcatg gaacggtctt tcatccacta ggctatctat tggccaaaac
     3421 atcaaagtct atgtaaaaac cgatcagtta tcgaaattca gcaatatgaa tactatgagt
     3481 tttgatgaaa agcaaaaatc atcaggttca tcttcgtcga ataaaactgt aacaggtgcc
     3541 ggaacccgcg aagatgccag ttatgaatac tatacggtgc agtcgggtga cagtccgtac
     3601 agcattgcaa aaaagtttcc cggaaccacg gctgagagca tcatgaagct aaataatatt
     3661 tcgaatgctt caggccttca aatcggccaa aaattgaaaa ttcggaagaa aaactaagct
     3721 tctttcaagt ataatagatt ttttacaata gaccttgttg cgcataaatt ttcatcaagt
     3781 tgttttttat cctgtggctt cagtacattt gtattttaaa ttttagaaga caatgtcagg
     3841 acaaagtagc tctaaaaaat cgattctgtt tgcatttgca gcaaacattg gaatagcaat
     3901 cacaaaaaca gttgctgccg taattacagg atcgggtgca atgcttgctg aagcaataca
     3961 ttcttatgcc gacagtggca atcaggtact cctatttgca ggtttaaggg cagcccgaaa
     4021 aaagccaaca cagacatacc cgctggggta cgggaagtca gtcttcttct ggtcgtttgt
     4081 tgttgcattg attcttttca gcgtgggcgg ccttttttcg gtatacgaag gcatccataa
     4141 actcaacagt cacaatggcg taagctcccc gcttattgca attgttgtgc ttagcataag
     4201 catacttctt gaagctgctt ctctctacgg ctgtattacc caaatacgaa aagtaaaacg
     4261 aaaaaacgaa aatttactgc aatggtttaa acacagcagg caaagcgaac tggttgttgt
     4321 gcttggcgaa gatgtcgctg ctttattagg tcttttattt gcacttatcg ctgtgctgtt
     4381 gacaatgctg acagggaatc ctgtttacga tgccgccggt agcataggta tcggggcatt
     4441 actcattata gtttctatat ttcttgctgt taagattaaa ggattactta tcggacaaag
     4501 tgctgatgaa aaaacggtgc tggaaataaa agaattcatt gaatcgcgcc acgaagttga
     4561 taccctgatg aacattatta cttttcagct gggacccgat atcatggttg ctgtaaaagc
     4621 aagaatgaaa gaaacgcaat cggcctcagt gttaattgat cacattaatt tatgcgaaag
     4681 agaaattaaa aaaaatttcc ccgatgtgaa atggatgttt tttgaaccgg acattgctga
     4741 tagtaagaat tagtaacctt tcagccccag atcaatcata tgcaccaccg gaatcataat
     4801 ttgccgcggg tcggatggat tgtatttaat ttgaaccttc ctgtcaatct ttaaatgcga
     4861 aacagcatca aaatgcgtca gtgaattcaa gtcatttttt ttgttcaagt catcgtaaat
     4921 cactgaagca caaacaatgc tctcattttg atcacaataa atcacactaa caatggtgca
     4981 atgaagtttc ccgaaaaact ctggccctct tttatgaaat accaccgcct ccgacttctc
     5041 ccaggaagaa tacctgtttt taaattttag aatttttgta gtgtaatagc caaacacatt
     5101 cattataaaa cggattatta aaaacgcagc taacgatact gcagcaagta ctatatatac
     5161 gatccaaata ttaggatatt tttctaccaa agcgaccaag caatatccca tgacaaatat
     5221 caatgcaatt gtaagcagct ttcgtgttct ccggctgagt acaggtgcat tcatagggaa
     5281 cgtacttgat ttaggcattg ttatatagct tgatgatagg atactgttct gcgataccat
     5341 gttcagtgat aatacaactt atatctgttg gttccaaaag atagatcata ttttgtcata
     5401 tttatatttc atctctgtat agtatgctgc ataatctgta acaaacgtgt cgccaaagta
     5461 ataccaccat ttattttcaa tatcgtagat gccaaaatac agcattgtat caattttgtt
     5521 atacgcttcc agcaaatcat tgaaagcttc tagcagctta tttgtcgtta cacatctgta
     5581 ggctgaatat aagtttccgt tatagagtgt aacaaagtca atcattttta ttcctgtctt
     5641 acgatagttt tttttcggat accaaacaat ccaactattt tttttgcacc aatcctgaca
     5701 ccaaacttta atcttttcat attcttcatc aatagccatt cttatgtttt caatggtgat
     5761 ttcggttgaa tttgacttat cctgactaag aatattattt attttattgc aataggcttc
     5821 catcgaaaag ccattgttaa tctccctgat tccatccttt ctgttatatt ttaagtagag
     5881 aaagtattgc agcagtgcat cgctaacagt aaacctaccg agttctttaa ctttttcgca
     5941 ttcaatcggg cggcttggat gcgtatcaac aaaattaagt acagtgtcga actcacctga
     6001 aaagaaagaa tccgcatatt tatttttgct agtacacaaa agcggataat taagcttagt
     6061 tacatgttcg gagcgagata gttttggctg tgcttttttt ttcggcaatc gcgaatcatc
     6121 cattaaggga tacacaacaa acaatttcag caacatgttt tttcgttgaa tcaaggcgat
     6181 gttttcagca tttacaggaa cattaaaacc gttttcggaa taaaatacaa atcgtttttc
     6241 tagatctaca tatgaccagg cactttgagt attaaacccc tcagcttgta atccgtgcga
     6301 ttccagttgc atacgtgttg ctttagagtt gtgtgaatat attgcataat ggttcatttc
     6361 cttttcattc caaccattgt caacaataat cttctttata gcagccatgg tctcaacctg
     6421 attgaacctt ctcgattcgg cttcggtata gaaaaaataa aagtaccatg ctttcgcata
     6481 attcatagct gcttcggtgg agtaatggcc gtagaagcgg ataccgtttg cttcactttc
     6541 aaaaatgtta attttttgct gaaagctcct gtccgcatca gcctgctttt taataccaag
     6601 tgttttattg ttctctttat tctgtttttg aaagccttta ttttgctgtg tttttcccac
     6661 ggattcaaat tcttgtactg cgtgttgcgt ttgaattaag cgggctttag tgatttcaca
     6721 ggcaattctt tgtacatctg ctgaatcaat tttaaattct tttatcaatg aatgcaatat
     6781 cgcagtgctt gttttattgt tttttgctgt agggctttca gcaatcaaac gtaagataaa
     6841 ttcaactcta aaaaacaccg ccagttcttt ttcgctgttg ttccattctg ccatatcctt
     6901 tcgcagagta tttatttcat gagaaaggtc attgcttttc gcaaggttta atgtaacacc
     6961 ttcataattg actacatagc tttcaccaag aaacagattc aaggcatctt tcaagtattt
     7021 cttacccatt tcgcgatgaa aagcgatgat gttaaccagc ttatttttat aggctatgtc
     7081 tttggtttga attagccaag tttttaaagc agagaaatta tcttcatctt cgagtaaaac
     7141 atcaataaag tcactcctgt tttctatttc caaggctaat tcgtgaacat tatgtatttc
     7201 aattccatcc acaatcagca ggttctcatc agtaaccagc tgctttcgac cggcttctgc
     7261 atccagaaac tttcccttaa gccaggcttc cacttcactc cacccccatc tgtcgtgata
     7321 ataagccatg gtaagtccgg taatgagacg gttcagcctt tcatattcag gagagaattt
     7381 taccagcgga ccgccattgt attgtttctc gatcagtatc gaaaattttt ctttatcgat
     7441 ttcgctaaaa ttctcgcttg tgcaaatttg ctccgggtac agcaggtgta ataatatcat
     7501 tccaaacgag tagtaatcgt tcttttcgga agtaataccc cgagcttgtt ccggagccag
     7561 atacgcttcg gttcccttaa cgattttcga cctacgcagt tccatttcgc tttgcaagtc
     7621 ccatgctttt gctgagccat aatccccgat caccaaatcg ttctgcgttt catcgagata
     7681 gagtatgttc tggggtttca agtcgcaatg gtatatttta tactcatgga gctttgtgat
     7741 agccttgaaa atttgcggga tcaattggtt ctcgaggaac agagctgtgt atttttgctt
     7801 aaaattagga acatccagta aattgccccc tctggcgtac tgcgatattt cgtaacaatt
     7861 tttcccgcgg aacttatcgg caccaactcc aaaatcatac agtttcaata tatcttcatc
     7921 gttgagcgat ttaattctct caagggcaat tccattgggt tcttctgcac cctgtaggta
     7981 gtcgtagtat agtttcaagg caaaatcttt actattcgag tcacgtatca ggtaaacaat
     8041 tgcttcgcca gtgccttcgg atagggttga caaaacattg tactccttat ttcgcaatga
     8101 tattttttct ccaggaatca gactgctttc agcaggttgc atgttgcctt tcaacaattc
     8161 gccattctgg ctttgtgcat tgtacaatcc ggttttctgg ttttgtaaat ctttgtaaat
     8221 ctttgtcttg tcattattat ccataaaaaa agtattatat cgggttccta tttaatgaat
     8281 acaaaatgga tggctttaag caattcaaca caagtgatta aaaatatccc tgtatattat
     8341 tatactatcc ctaggctatt cggaatcaag ggctgaggtt actgcaaaag cacgaaagca
     8401 acaatttgga aaacattttt aaacttcatg gttattaggt ttgatatatt tagctaaaat
     8461 acagtttttt caatttttgt gcaatttctt atgcaatatt cttgtaactc cgacggaatt
     8521 actttaaggt aatatttaca attaataaga atagaatcca tacattttcg aagcaaaaat
     8581 tattttttta caattgctac aattacataa ttgctgtatt gataaatcga atttttatga
     8641 atacaaatac ttttctattt ttgtaatata aaatcaagca ttatgaaaaa tatcaccgtt
     8701 aagcatgtaa tacttgccat cagcgtactg ctgcttttga ttttcacttt tcaaaacctt
     8761 gaaacagtaa gggttcattt ccttttcttc gattttggga tgcccttggt aattcttatt
     8821 gttcttgttt tttttattgg tttttttact gcatgggtaa tgaagaaact gaagaagaaa
     8881 aacattgatt tacggtaact cattcttt
//
LOCUS       DAOOQP010000121         8860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9777_length_8860_cov_5.312095, whole genome shotgun sequence.
ACCESSION   DAOOQP010000121 DAOOQP010000000
VERSION     DAOOQP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9777_length_8860_cov_5.312095"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLK75_10855"
     CDS             complement(<1..184)
                     /locus_tag="PLK75_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39785.1"
                     /translation="MNCTGIEILQQLKLLTVLMGVCTGSLCAASPDNDTLPSLPAMHD
                     YIQGVDADVALSADYRI"
     gene            complement(192..929)
                     /locus_tag="PLK75_10860"
     CDS             complement(192..929)
                     /locus_tag="PLK75_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY39786.1"
                     /translation="MKKLKTAVLIPTYNNASTLNSVISSVLNYCSDVIVVNDGSTDNT
                     ESIIAEYKGRIHAISYSPNKGKGFALRAGFAYAAKNSFDNVITIDSDGQHSAADILLF
                     AETAQENPGALIIGSRKLVQENMGGKSTFANRFSNFWFLVQTWKKLPDTQSGFRLYPI
                     STMRGMRFITRRYETELEILVLSAWRRIRLIPIPISVYYAPPDEKVSHFRPFHDFFRI
                     SVLNTVLVIFSIVYGYPSMLIRKIFQK"
     gene            complement(959..1354)
                     /locus_tag="PLK75_10865"
     CDS             complement(959..1354)
                     /locus_tag="PLK75_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39787.1"
                     /translation="MTNRRTDNYFLNDLYTIDSIGAAGNSLTASVMFNPSHWIYSCHF
                     PGNPITPGVCVVQVARELVSTHLNKALHIRMLKSIKFLNIINPETDGPIRFLFTLHEE
                     NDNTIRAEVNVENSTTVVVKMNIILCQER"
     gene            complement(1351..1941)
                     /locus_tag="PLK75_10870"
     CDS             complement(1351..1941)
                     /locus_tag="PLK75_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39788.1"
                     /translation="MLTRALLILCFASTSLLGFTQISGSENDANTAMRRFVSIFPFED
                     GKSLNFQASVEHINFYITGVVVAKMMGDTVKGVFMNEFGIKVFEFEVLRGRCRLMQVL
                     DIFDKWYFRKTIRNDFAFIFTVLEKPSEAVGVYEFPFRGEKNVYRYRFEHEKIATLLR
                     IEKGKTTAVLECGSDSVFTFRNTDRNISYKFNIVPK"
     gene            complement(1898..2554)
                     /locus_tag="PLK75_10875"
     CDS             complement(1898..2554)
                     /locus_tag="PLK75_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOY39789.1"
                     /translation="MKSIVLKAVFLAIAHVVVFHALSFSQNLKPATELQKSEIIKKMQ
                     SAALSTNSMECKFVQSKTLSVLAETVTSEGKMYFKKENKLRWQYEKPYSYIYILSEGK
                     VYIKSEGNTSTFDTNSNKMFKEISEIMIGGVRGNLLLNNKKFSAQYFMGADKAVIKLT
                     PTGAELKNMISSINLTVSTSDWLVKSIEMVEKGGDITLITFTEKNVNKSIADTLFRLN
                     "
     gene            complement(2574..2927)
                     /locus_tag="PLK75_10880"
     CDS             complement(2574..2927)
                     /locus_tag="PLK75_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOY39790.1"
                     /translation="MGKLIFYASVQSLFLVISQIFLKLAVARSGNMSFTWSYIKEIVT
                     TWQMICSGLSIMIATIIWMYIFKHYEFSQAYPLISISYVFAIIAAVVIFHEAVPFTRW
                     LGIALIIVGVIFVTK"
     gene            complement(2915..3985)
                     /locus_tag="PLK75_10885"
     CDS             complement(2915..3985)
                     /locus_tag="PLK75_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOY39791.1"
                     /translation="MKVKNAYINGASVISIQPEFSIAMLESPATYHEPYARCIDPSFR
                     DYFDPLAARRMSRIIKRAIITARKAVAEASIEIPDAIITGTGLGCVEETEKFLDSMIR
                     NEEKFLQPAFFIQSTHNTISSQIALSMGCKGYNNTYIHRGVSFENALMDALLQLNATR
                     VGNVMVTGNDEMTPNYFTLLKRIGYWKQEVPDTLKIIGDCSTPGSFAAEGSVSMIISE
                     APASTTYARIEAMKLDYKPQGFDSNDISVFLTENNCTPNDIDVFLTGMNGDIENDKVY
                     KGLFSFFGEDKIAFYRNICGEYFTSSAFGILAAALCVKSGKVPASMTLKGRPVEKVRR
                     VLFYNHFKNKDHSLILLTTWAN"
     gene            complement(3982..5184)
                     /locus_tag="PLK75_10890"
     CDS             complement(3982..5184)
                     /locus_tag="PLK75_10890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOY39792.1"
                     /translation="MPETIVVSGMGIVSGIGNNQQETLCSLLENRSAISSLKHIKSIH
                     ELPCAEVSFTDEEMKKMLCLEPDSVYTRTTLLSMLAVREALQQAKLEDVKNIRAAFIN
                     GTTVGGMEKSEIYYFDFLNTDLYNEYIEAHDCGACTEKIADYFGGFCFVSTPSTACSS
                     AANAIALGADLLKAGVVDVAVVGGTECITKFHLNGFNSLLILDKQICRPFDETRAGLN
                     LGEGAAYLVVEFEQQSKKRNVQPLAILSGYGNACDAFHQTASSPNGDGAVMAMLKALT
                     NAGLLPADIDYINAHGTGTPNNDESEGNAIVRIFGDKPPYISSTKSLTGHTTSAAGGV
                     EAVVSILALQNDFVPANFNFSQKMLSLPFEPVKQVILNKELHHVLSNSFGFGGNNSSL
                     IFSKYKGK"
     gene            complement(5186..5443)
                     /locus_tag="PLK75_10895"
     CDS             complement(5186..5443)
                     /locus_tag="PLK75_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOY39793.1"
                     /translation="MDELVLQLKKEIIEVLNLEEITPDDIDENAPLFGEGLGLDSIDA
                     LELIVLLEKNYGIKLQDANEGREIFKSISSMADYIQKNRKI"
     gene            complement(5454..6077)
                     /locus_tag="PLK75_10900"
     CDS             complement(5454..6077)
                     /locus_tag="PLK75_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39794.1"
                     /translation="MILTIQSYTRISNYTLIINGNDVFASPESESYTSVLTAIYRHLQ
                     VQYPKFFKMDNLSKTGFLSVEALFNKKNEEAALDGSNTPVFLICKSSSLETDCNYQLT
                     LAEGNIPSPSLFVYTLPNILLGELAIRFKLFSENTCFVSENYLNENVFDYICQSCLTS
                     DIQKAIVLFADQYGNNGEVLALLVEKTNNKMYEGLVFDIETIKSLIK"
     gene            complement(6074..7240)
                     /locus_tag="PLK75_10905"
     CDS             complement(6074..7240)
                     /locus_tag="PLK75_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOY39795.1"
                     /translation="MAVVYQISDNIISPLGLTTHENYVAVVNGQHGLRKFSNDYLEVS
                     EDFCAAIIDDCNTYTMRQKFGLLDKYTRAEKLSILSVALAMENSGIDASSERTLFVVS
                     TTKGNISVLEGNFNREHPDKRLYLWHLAQEISDYFNNPNTPVVVSQACISGISAQIVA
                     KRLILSGRFDHAIVVGIDLVTRFVVSGFQSFKALSPDPCRPFDAERKGLNIGEAAATI
                     IYGKANSITELPENAIYLDAESVTNDANHISGPSRTGEGLFQAIQKAMLQSGNTLPDF
                     ICAHGTATPYNDEMEAIALTRAGLLSVPVFSLKPYFGHTLGAAGVLETILASEALKNN
                     MVLRNMNYTSSGVSAPVNVVTSNSKLRANSFLKTISGFGGCNAAAIFKKKGGAL"
     gene            complement(7244..7663)
                     /locus_tag="PLK75_10910"
     CDS             complement(7244..7663)
                     /locus_tag="PLK75_10910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOY39796.1"
                     /translation="MTKLTVRKEIEIRFNEVDSMGIVWHGNYATYFEDAREEFGRKYN
                     LGYLRMFAEGFYTPLVSLDFSYKHPLIYGDKAIVEIVYIPVEAAKICYKYKIYSLPKN
                     TLIATGSSIQVFLDKNYQLIWNNPDFYIEWKKNNGLT"
     gene            complement(7660..8073)
                     /locus_tag="PLK75_10915"
     CDS             complement(7660..8073)
                     /locus_tag="PLK75_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridylate synthase"
                     /protein_id="HOY39797.1"
                     /translation="MRNIDIRTLLPQQYPFVLIDRLVAFDLVITKTEFVVPEDYVFVR
                     EGKLTEPGIIENIAQTCAARMGYRNMVLYSGDIKLGYIGAIKNLTIQRLPFSGEMLET
                     TIEVLSEVLNMLLVKATVLSGGTLIAECEMKIAEQ"
     gene            complement(8083..>8860)
                     /locus_tag="PLK75_10920"
     CDS             complement(8083..>8860)
                     /locus_tag="PLK75_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOY39798.1"
                     /translation="NDDLTRVNMDFTHDVNYLGFFRLLESVKNGRPEGLQVFGKWPTV
                     YEALSELPEQVRKSWFGFDHYYSDASFPMVLPIIFADKPSKIMDVGGNTGKFAIQCAA
                     YNPDVKITILDLPGQLSDAYKNIRERGYENRIDGYAINLLDHSKPYPKNYDVVWMSQF
                     LDCFSQYDVVELLKKAKDAISETGYVYIMETFWDNQRTDAGKYCLVATSLYFTCIANG
                     CSQMYHTEDMYAMIEKAGLKVAETFNNIGVSHTVLKCVTA"
BASE COUNT         2639 a         1941 c         1632 g         2648 t
ORIGIN      
        1 taatcctgta gtcagctgat aaagccacat cagcatcaac accctgaata taatcatgca
       61 tagccgggag cgatggaagg gtgtcgttat cgggggaagc agcgcataaa gaaccggtgc
      121 aaacacccat aagtaccgtg agcagcttca attgttgtaa tatttcaatt cccgtgcaat
      181 tcattcaaca ttcatttctg aaaaattttc ctgatcaaca tcgaaggata gccatacact
      241 attgaaaata tcaccaatac tgtgttcaaa acacttatac ggaagaaatc gtgaaatggt
      301 cggaagtgcg aaactttttc atcggggggt gcataatata cactgatagg aattggaata
      361 agccggattc tccgccaagc cgaaagcacc agaatctcaa gttcggtttc gtatcgccgc
      421 gttataaacc gcatacctcg catcgtggaa atcggatata agcgaaaccc cgactgggta
      481 tcaggcaatt tcttccacgt ttgaaccaga aaccagaaat tagaaaaacg gttggcaaaa
      541 gtgcttttcc cacccatatt ttcctgtacc agttttcggc taccaattat caaagccccg
      601 ggattttctt gcgcagtttc agcaaacaac aaaatatctg cagcactatg ctgaccatcg
      661 gaatcgatag taatcacatt atcaaaggaa ttcttagccg cataggcaaa ccccgccctc
      721 aacgcaaagc ctttgccttt gttaggagaa tacgatattg catgtatccg gcctttgtac
      781 tctgcaatta tcgattcagt gttatcggta gaaccatcgt taaccacaat tacatcgctg
      841 caataattca gcacagaaga aatgacactg ttcagcgtgg aagcattgtt atatgttggt
      901 attagcacgg cagtcttcag ctttttcaaa atactgcagc aattttgcac atccttactc
      961 atcgttcctg acaaagtata atattcattt tcacaacaac ggttgtgctg ttttctacat
     1021 tcacttccgc tcttatggta ttatcgtttt cttcatgcaa tgtaaataaa aaccggattg
     1081 gtccgtcagt ttcagggttt atgatgttca ggaatttaat actcttcagc attctgatat
     1141 gcaaggcttt gttaagatgg gtggaaacaa gctctctggc aacctgaaca acacatacgc
     1201 ccggagtaat gggattgccg ggaaagtgac aggaataaat ccaatgagac gggttaaaca
     1261 tcaccgatgc tgtcagagaa tttcccgcag caccaatgct atcaatggtg tacaagtcgt
     1321 tcagaaaata attatccgtt cttctatttg tcatttcggt acaatgttaa acttataaga
     1381 tatattccta tcggtattac gaaaggtaaa aactgagtct gaaccacact ctaacactgc
     1441 agtagttttt cctttttcaa tacgaagcaa tgtagcaatt ttttcatgtt caaaccgata
     1501 acggtacacg tttttttccc cccgaaaagg gaattcatat acaccaactg cttccgaagg
     1561 tttttctaaa accgtgaaaa taaaagcgaa atcatttctg attgttttgc ggaaatacca
     1621 cttgtcgaaa atatcaagta cctgcatcaa gcggcacctc ccgcgcagca cttcaaactc
     1681 aaacactttg atgccgaatt cattcataaa aacaccttta acggtgtctc ccatcatttt
     1741 agcaaccact accccagtga tataaaaatt gatatgttca accgacgctt ggaaattcaa
     1801 tgatttacca tcttcgaacg gaaaaatcga aacaaagcgg cgcatagcgg tattcgcatc
     1861 attttcactt cccgaaatct gagtgaatcc caaaagacta gttgaggcga aacaaagtat
     1921 cagcaatgct cttgttaaca tttttttcgg tgaaagttat cagcgttata tctcctcctt
     1981 tttcaaccat ttctattgat tttaccagcc agtcggatgt tgaaacagtg aggttaattg
     2041 atgaaatcat gttttttagt tcagcaccgg ttggtgtaag cttgattact gccttatcgg
     2101 cacccataaa atattgagca ctgaattttt tattattaag caataaattg cccctcaccc
     2161 cccctatcat tatttcgctg atctctttga acattttgtt cgaattcgta tcaaaagtgc
     2221 tggtatttcc ttcactttta atatacactt ttccttcgct taaaatataa atgtagctat
     2281 atggtttttc atactgccac cgcagtttgt tttctttttt aaaatacatc ttcccctcgg
     2341 aagtcactgt ctcagcaagc accgacaagg ttttactttg aacaaattta cactccatac
     2401 tattggtaga gagtgcggca gattgcatct ttttaattat ttcgctcttt tgcaattcgg
     2461 tagcgggttt cagattctga gagaatgata aggcatgaaa cactacaaca tgagctatag
     2521 caagaaaaac agcttttaaa acaattgatt tcatcgatac gtttttataa caactatttt
     2581 gttacaaaaa ttactccaac aattataagg gctatcccga gccaccttgt gaaaggtact
     2641 gcttcatgaa aaattacaac ggctgcaatt atggcaaata cgtagctgat actgataagc
     2701 gggtaagcct ggctgaattc gtaatgttta aaaatataca tccagatgat tgtggcaatc
     2761 ataattgaca agccggaaca aatcatctgc catgttgtaa caatttcttt aatatacgac
     2821 caggtaaaac tcatgtttcc tgacctcgcc actgccagtt ttagaaatat ttgagaaata
     2881 accaaaaaaa gcgattgcac cgaagcgtaa aatattagtt tgcccatgtt gtcaatagaa
     2941 taagtgaatg atctttgttt ttaaaatgat tataaaataa taccctgcgt actttttcta
     3001 caggtcgtcc tttgagggtc attgaagcag gaactttacc cgatttcaca cacaaggctg
     3061 ctgccagtat tccaaaagca gatgaagtaa aatattctcc gcatatattt cggtagaatg
     3121 ctattttatc ttccccgaaa aagctgaaga gacctttata gaccttgtcg ttttcaatat
     3181 caccattcat tccggtaaga aaaacatcaa tatcattggg agtacagttg ttttcagtta
     3241 ggaatactga tatatcgttg ctatcgaaac cctgaggttt ataatcaagc ttcatagctt
     3301 cgattctggc ataggtggtt gatgcaggtg cctctgaaat tatcatacta acactaccct
     3361 ctgctgcaaa cgaccctggt gtggagcagt cgccaattat tttcaaagta tcgggaactt
     3421 cttgtttcca atatccgatt cttttcagca gggtaaaata attgggcgtc atctcatcgt
     3481 taccggttac cataacatta ccgacccgtg ttgcgttgag ttgaagtaag gcatccatca
     3541 aagcgttttc aaacgataca cccctgtgaa tatatgtatt gttgtaacct ttgcagccca
     3601 tactgagagc tatttgtgag ctaatggtgt tatgagttga ctggataaaa aatgcaggct
     3661 gaagaaactt ctcttcattg cgaatcatcg agtctaggaa cttttctgtt tcctctacac
     3721 atcccaaacc ggtacctgta ataattgcat cgggaatttc gatacttgct tcggcaaccg
     3781 ctttacgtgc tgtaataatt gctcttttta taatccggct catacgacgg gcagcaagag
     3841 ggtcgaaata atctcggaat gaagggtcaa tacatcttgc atacggctca tgataagtag
     3901 ccggtgattc gagcatcgca atgctgaatt caggctgtat cgatattaca gatgccccgt
     3961 tgatatatgc gtttttaacc ttcatttgcc tttgtatttc gaaaaaatca aacttgaatt
     4021 attcccgcca aaaccaaacg aattcgaaag cacatgatgc aactctttat tcaggataac
     4081 ctgtttaacc ggttcaaatg gcaagcttag catcttctgc gaaaaattaa aatttgcagg
     4141 gacaaaatcg ttttgtaaag ccagaatcga aacaacagct tcaactccgc ctgcggcact
     4201 tgtagtatga cccgtgagcg attttgtaga actgatataa ggcggtttat caccaaatat
     4261 tcttacaatc gcattgcctt ccgattcatc gttattgggg gttcctgtgc cgtgagcatt
     4321 gatatagtcg atgtctgccg gcaacaaccc tgcgttggtc agtgctttca gcattgccat
     4381 aacagcacca tcgccattgg gtgacgatgc ggtttgatga aaagcatcac aggcattccc
     4441 atatcctgac agaatagcca aaggttgaac attgcgtttt ttgctttgct gttcaaattc
     4501 aaccaccaga taagcagcac cttcgcctaa attcagtccg gcacgggttt cgtcaaacgg
     4561 acggcagatc tgtttatcga gaatcaacaa agaattaaaa ccattcagat gaaattttgt
     4621 aatgcattct gtgcccccaa caacagcaac atccactaca ccggctttca acaggtcagc
     4681 accgagcgca attgcatttg ctgccgatga gcatgctgtc gaaggcgttg aaacaaaaca
     4741 aaaaccgcca aagtaatcag ctattttttc ggtacatgca ccacaatcgt gggcttcaat
     4801 atattcatta tataaatcgg tattcagaaa atcgaaatag tatatttcgc ttttctccat
     4861 ccctcccaca gttgttccat taataaatgc agccctgata tttttaacat cctccagctt
     4921 tgcctgttgc agagcttcgc gcacagcaag catcgatagc aatgtcgtac gagtatacac
     4981 cgaatccggt tctaggcaca acattttttt catttcctca tccgtaaacg acacttcggc
     5041 gcacggcaat tcatggatac ttttaatatg tttcaggctg cttatggcag aacggttttc
     5101 aagcagagaa cacaaggttt cctgctgatt gtttccgata ccggaaacaa ttcccattcc
     5161 gctcacaaca atggtttcag gcatatcaga tttttcggtt tttttgaata taatcagcca
     5221 tgctgcttat cgatttaaaa atctcgcgac cttcgttagc atcctgaagc ttaataccat
     5281 agtttttttc gagcaacact atcagttcca gtgcatcaat agagtcaagt ccaagccctt
     5341 caccaaacag aggagcattt tcgtcgatat catcaggggt aatctcttca agatttaaaa
     5401 cctcgataat ttcctttttc agttgtaata ccagttcgtc catataaata tacttattta
     5461 attaaacttt ttattgtttc aatatcaaaa accaagcctt catacatctt gttgtttgtt
     5521 ttttccacca acagtgccaa cacttctccg ttgtttccgt actgatcagc aaagagcaca
     5581 atcgcttttt gtatatctga agtcaaacaa ctttgacaaa tataatcgaa aacattttca
     5641 ttcaaatagt tttcggaaac aaagcaggtg ttttccgaaa atagtttaaa gcgaatagca
     5701 agctctccta aaagtatgtt aggcaatgtg tacacaaaaa gcgacggact cggaatattg
     5761 ccttcggcaa gtgttagctg atagttgcaa tccgtttcaa gcgaagatga tttgcaaatt
     5821 aaaaatactg gtgtatttga accatcaagc gcagcctcct catttttttt gttgaataat
     5881 gcttccaccg ataaaaagcc tgtcttcgac agattgtcca ttttaaaaaa tttcgggtat
     5941 tgaacctgca gatggcgata tattgcagtg agaactgatg tgtaactttc actttcagga
     6001 gaagcgaaca catcgttccc gttaataatc agcgtgtaat tcgaaatacg ggtgtagctt
     6061 tgaatcgtaa gaatcataat gcaccccctt tctttttaaa tattgctgct gcgttacaac
     6121 cgccaaaacc ggaaatagtt ttcaggaaag agtttgccct gagctttgag ttggatgtaa
     6181 ccacgtttac aggtgcagaa accccgctgg aagtgtagtt catatttctg agaaccatgt
     6241 tatttttcaa tgcttccgaa gcgagtatgg tttcgagcac tccagcagca cccaatgtat
     6301 gtccgaaata tggtttcagg ctaaaaaccg gtaccgacaa caaacctgca cgtgttaaag
     6361 caatagcctc catttcatcg ttgtaaggtg tagcagttcc atgggcacaa ataaaatcag
     6421 ggagggtatt ccccgattgc agcattgctt tttgtatagc ctgaaacaag ccctctcctg
     6481 tgcgtgaggg acccgaaata tgattggcat catttgtaac gctttcggca tcaaggtaga
     6541 tagcgttttc gggaagctcc gtaatagaat ttgctttgcc atagataata gttgcagcag
     6601 cctcaccaat attcaggcct tttcgctctg catcaaatgg tcggcaggga tcaggcgata
     6661 aggccttaaa cgactgaaac ccacttacaa caaaacgcgt aacaaggtca attcccacta
     6721 caatggcatg gtcgaagcga ccggaaagaa tcagtcgttt tgcaacaatt tgtgccgaaa
     6781 tgcccgagat acaagcctgt gaaactacaa ccggcgtatt ggggttattg aaatagtctg
     6841 aaatttcttg ggccaggtgc cataaataca agcgcttatc agggtgttcc ctgttgaaat
     6901 taccctccaa tactgatata ttcccttttg tggtagaaac aacaaaaagt gtcctctccg
     6961 atgatgcatc aattcctgaa ttttccatag ccaatgctac ggacaaaatc gacagttttt
     7021 cggcacgcgt atatttatcc agcagaccaa acttttgcct cattgtatac gtattgcagt
     7081 cgtcgattat ggcagcgcaa aaatcttcag aaacttccaa ataatcattg ctgaattttc
     7141 tcaaaccgtg ttgcccatta acaacagcca cataattttc gtgggtagtt aacccaagag
     7201 gagaaataat attatccgat atttgataaa cgaccgccat tttctacgtc aatccattgt
     7261 tttttttcca ttcaatataa aaatccgggt tgttccagat aagttgatag ttcttatcca
     7321 gaaaaacctg aatgctgcta ccggtagcaa taagtgtatt tttcggaagc gaataaattt
     7381 tgtacttata acaaattttt gcagcttcta cagggatata cacaatttcg acaatggctt
     7441 tatcaccata aatgagtggg tgtttatagc taaaatcgag gctaaccaat ggggtataaa
     7501 acccttccgc aaacattctc agatatccaa gattgtattt tctcccaaac tcttcgcgtg
     7561 catcttcaaa atatgtagca tagttaccat gccaaacaat tcccattgaa tccacttcat
     7621 tgaagcgaat ttcaatctct tttcttacag taagtttcgt cattgttctg caattttcat
     7681 ttcgcattct gcaattaatg ttccacctga taatactgta gcttttacca agagcatatt
     7741 aagtacttcc gacaatacct caatggttgt ttcaagcatt tctccggaga aaggcagccg
     7801 ttgtattgtc agatttttaa tggctccaat atatcccagc ttaatatcgc cactataaag
     7861 caccatgttc cggtaaccca tcctagcagc acacgtctga gcaatatttt cgattatacc
     7921 cggttcggtc aattttcctt ccctaacaaa cacataatct tcaggtacaa caaattccgt
     7981 ttttgtaatt accaaatcga aagccaccag cctgtcgatt aatacaaacg ggtattgttg
     8041 aggcaatagg gttcttatat caatatttct catattttta aactatgcag taacacattt
     8101 cagcaccgtg tgacttaccc caatgttgtt gaaggtctct gcaactttaa gtcccgcttt
     8161 ttcaatcatg gcgtacatat cttcggtatg gtacatctgg ctacaaccgt ttgcaataca
     8221 ggtaaaatac aaacttgttg caacgaggca atacttgcca gcatcggtac gctggttgtc
     8281 ccagaaagtt tccatgatat atacatatcc ggtttcagat attgcatcct tggctttttt
     8341 cagtaactca accacatcgt attgcgaaaa gcaatcgagg aactgactca tccacaccac
     8401 atcgtagttt ttcggatacg gttttgagtg atccagcaaa tttatggcat aaccatctat
     8461 cctgttctca tagccgcgtt ccctgatatt tttataagca tcgcttagtt gccccggcaa
     8521 atcgagaatg gtaattttta catctggatt ataggcagcg cattgaatgg caaatttacc
     8581 cgtatttcct cccacatcca taattttcga tggtttatca gcaaaaatga taggtagaac
     8641 catggggaaa gaagcatcgg aatagtaatg atcgaaacca aaccaacttt tgcgaacctg
     8701 ctcaggaagc tccgataagg cttcgtatac agtaggccat ttgccaaaaa cctgtaagcc
     8761 ttcgggccga ccgtttttca ctgattcaag caggcgaaaa aaaccgagat aattgacgtc
     8821 gtgggtaaaa tccatattaa cccgtgtaag gtcatcgttc
//
LOCUS       DAOOQP010000122         8754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9925_length_8754_cov_5.343718, whole genome shotgun sequence.
ACCESSION   DAOOQP010000122 DAOOQP010000000
VERSION     DAOOQP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9925_length_8754_cov_5.343718"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1214
                     /locus_tag="PLK75_10925"
     CDS             45..1214
                     /locus_tag="PLK75_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39799.1"
                     /translation="MKKRFTLLLSFILVSLIGFTQILPENFTVRNNVGQLKGLKSTKA
                     LKQRLDQIVYEAYSWDTQQWSNDAKVTYTYNDNGNVTSAITFDYSGGTWVNDLKEDCS
                     YNGEGDIVQVMESDWDGTAWQLSKKYDYTYNGSGYIETKITSIYQSGNWVNSLKSEYT
                     YDGNGKMTLDFVSAWSTAKGWQTDSKYEYAYDINQRISEIVRSTIDVGMGEWELNKRN
                     AFTHDENGNLTRRDDSMWIGSWNVYQYEEFTHSNTYTLADLLLPYSWLLQYFYFAHQL
                     TGTIRYVYNYDVVNFMQFSRSTYIYSQQNVGITDNVVPVYSVYPNPVTDKLNFTLPEN
                     TQNAMVQLSDLYGRKVLECTVSNNQSISVNHLASGMYICNITDGNFCYTCKLQIH"
     gene            complement(1349..2641)
                     /locus_tag="PLK75_10930"
     CDS             complement(1349..2641)
                     /locus_tag="PLK75_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOY39800.1"
                     /translation="MKTLLPLLQDADLKDKVVLVRVDHNVVKKGQIHDPYRIDATFGT
                     LFHILAKGGKIILMTHVGRPKDKKTGAIKIDEESSVQPIVEYLQNKLHVKIKVPEFYV
                     HGDTGYAGIETSVNHLIRQLRNHEIDMIYLPNTRWFTGEEAKDESAERLANQMAGLAD
                     IYVNDAFGSWQAHVSTVGISKYLPSYAGFLLQKEIESLDRIYDAQKPMVAVVAGSKFD
                     TKIDSLNALIQKADYLVLGGVIYNAYLAAKYGISITGIEDDDIRLAKEFITKSEKYAD
                     RIVELPYIVESDVFEGKIEGKNRVHDIRKLKSGAKLNYVLDIAPESFEDAHVKEVFNK
                     ANTIFVNAVMGYTPHFNDGTIALDSLIDTNINAVKLYGGGDTMQELKRLLPGLYIMAL
                     DSPKYYIFTGGGAVLKAIENGTYLGLEPVKALINGKTQ"
     gene            2728..3750
                     /locus_tag="PLK75_10935"
     CDS             2728..3750
                     /locus_tag="PLK75_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39801.1"
                     /translation="MSIRLPYISVEVNENCNQSCCFCYNNKVSQSEYHQSNYAQLRRL
                     LVTLYKQARPERVVFTGGEPLLEPRIAEIVLFCKMRKSAVSIITNGTAATKNVYKDLI
                     LLQTDLFQLPVHSFNAEAHDKMTGLKGSWEKSVNSILQIKSLGGRVVPVIVITRHNVK
                     EIDKTVQFIISLGIKELMLNRFNFSENAGKEAAGMSASKSELNQAYTAVNTLARDNAM
                     KVSSNVNTPYCVLRPSEYPFIRFSGCASDILRNPITTDIHGNLRKCNHSNVVAGNIFR
                     TSIEEILRSDYLKSWNEIIPDFCKKCSDWPQCRGGCRAAAERTGGNISLPDPMVLLLS
                     ENQQLV"
     gene            3854..7606
                     /locus_tag="PLK75_10940"
     CDS             3854..7606
                     /locus_tag="PLK75_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY39802.1"
                     /translation="MKKINGLFALMFCCLMAHSQEANLILPEGHLEKIIQIGYSPDGN
                     YLVSASDDNTIKVWDVRTGRLLHNLSDHTDDILKFVFIESGTKIRSLSRDKTVRTWNI
                     KTGELEFVSSGSKVVNTRAFINEKGTLVATTDMNDGIARIWDVTGKEWVLKYELPLNA
                     ESPLYLVCFSDNDKYVCTNYEEGKAAVWDLESGEKRFVTKNKGIIFTASFSNDNRKLI
                     TIISDTLSVWNIETGKLSFEVKKNNGCISCAKYSNNDSIIITGSTRGELRIFEGATGK
                     LIRKIPANIDEISDIAISSDSRFVYSVDYEGLVTKSDLSTGKLISEFSTLYGSNSTMR
                     FRLRLSLSPDEKTLAVTNFAGTVEIWNIADNRILHELKGQTSKIKKPVFSPLGDIVAV
                     VSADFTVKVWNYKNASLLHSLKGHQTPIETLTFTPDGSKIITTSEDGFLKVWDVSTGR
                     LLFSIEDLYSRIDDVLISPNGKYLFILPRWKNPRVYNILSGELLFELMIESSKFRGAY
                     YSDSGSYLYVFYRENDQIKYLEINVTKSIAIDDIPVGEIENHEFDGHKTVLLLPDKSG
                     LLYLDEFELNYCSLTDKNKNVVFSAHPMEITKDPFNNNGKLIVFDTYDGTDELHAQVC
                     DIETGKTVYDLSGYTFNPNGGIEQPFSSDARWILTMDDSGFVRVWNAADGKLKTEIKC
                     GDVNNILAFARFMPDNNTVMAGSFKVNEMQYIYHFNPVGIYDIETGNLIGTIPFEQGF
                     PAAFCDSENIAAYCNNSQLVLYDTKTCKQNASYIALDNSEYIVITPEKYYKCSKNAAG
                     KVLWRAGGKFYRFDQFDLQYNRPDIVLEKVGNPDTVLIKLYREAYKKRLRKSGFSENV
                     FQPGWSCPEVEILNASTFPWITDKSKITLNIVAEDKNASLNRLNISVNEVPINGVTGI
                     GIKTDNLPSVENWVEVDLCEGYNIIEVSCTNEFGVESFKENLEINYVPAVPHQPILHL
                     IAVSVSEYADGSFNLTYPVKDGRDMVALLGNGTSGTSAIAVVDTLFNAMATRSNLLDM
                     KTDLGKTNIDDIVILYLSGHGLLSDNLDFYYATYNVDFARPELQGISYDELEWLLDGI
                     PARKKLILIDACHSGELDRDYVDQTNDSVILADGTRSGLKTYSYKTAMMPSQQGNIPF
                     NSAFELMQETFNDLSKGSGAVVISAASGTGFAIESPVWNNGVFTYAVISGLKELRADL
                     NNDNMITVSELKVFVSNEVESLTRGRQKPSGRGESLLFDWKLVQKE"
     gene            7733..>8754
                     /locus_tag="PLK75_10945"
     CDS             7733..>8754
                     /locus_tag="PLK75_10945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY39803.1"
                     /translation="MKTLRIAVVLIIAAFSTYMLTSCDPESAEPTITWEGIISYNYDA
                     NEDGNYAHTLNLVVDAEAGINLFNIWKHVITDTDTVSSLVDSPADCIGLLTYNHDLDI
                     AVTPADVAGGVTKIIYEMEVTDNDLNTATEEFVIFVTNGTLSYNVTFVVKDALGGDIA
                     DAIMVFGDSTNAAGDYTLVGVPAGTYNYTVNKAGYDEKAGTGFVLASDTTVTIELQQE
                     WSAEVPLALYGQTTWATYQGNPVTVYYSEVFGVAVDFTNAGILRIIDTKHDGTTLGCD
                     GWVVVNAADVAAMNTQAALATAYAAGTVIHTYDLQCDQHKNYEELNFISKMGSTYKIV
                     RYVAGYR"
BASE COUNT         2690 a         1671 c         1841 g         2552 t
ORIGIN      
        1 atttttgaat ttataagtcc tatgtcgaac caaaacaaaa cattatgaaa aagagattta
       61 cacttttgtt atcttttata ctggtaagtc tgattggatt cacccagata ttgcctgaaa
      121 atttcacagt tagaaacaat gtaggacaac tgaagggttt gaaatcgacc aaagcactga
      181 aacaacgcct tgatcaaatt gtgtacgaag cctattcgtg ggacacacag caatggtcga
      241 acgatgcaaa agtgacatac acttacaatg acaacggaaa tgttaccagt gccataacat
      301 tcgattattc gggaggcaca tgggtaaacg accttaaaga agattgctcc tacaatggcg
      361 aaggggacat tgttcaggtg atggaatcag actgggatgg tactgcatgg caactcagca
      421 aaaaatacga ctatacctat aatggcagtg gatatatcga aacaaaaatt acttccatat
      481 atcaatcggg taattgggta aattctctaa aatcggaata tacttacgat ggaaatggga
      541 aaatgactct cgattttgtt tcagcctgga gcacggccaa aggatggcag accgattcaa
      601 aatacgaata tgcatacgac attaaccagc gaatttcaga aatagtccgg tcaacaattg
      661 acgttggaat gggtgaatgg gaactaaata aacgcaatgc ttttacccat gatgagaacg
      721 gtaacctgac ccgccgcgat gattccatgt ggattggtag ctggaatgta tatcagtacg
      781 aagagtttac ccattcaaat acctataccc tggcagatct tttgttaccg tattcatggt
      841 tgctgcagta tttttacttt gcacatcagc ttaccggaac tattcggtat gtgtacaact
      901 acgatgtggt aaactttatg cagttcagta ggagcaccta tatctattcg caacaaaatg
      961 tggggattac cgataatgta gtacccgttt attcagttta ccccaatccg gttaccgata
     1021 aactgaactt taccctgccc gaaaatactc aaaatgctat ggtgcaacta tccgacctct
     1081 atgggcgtaa agttcttgaa tgcaccgtta gcaataatca aagcatttcg gtaaatcatc
     1141 tggcttccgg aatgtacatt tgtaatatca cagatggtaa tttttgctac acctgcaagc
     1201 tgcaaatcca ctagattttt agcttttacg gcgaaatgtt gctcttttat tgaataggca
     1261 aaaaacttct tagtaaacaa gttgcgttgc ggctaaaatt tcacatgaat ttgggccaaa
     1321 ttttcgtgcg atttgccgaa attgtaactt actgagtttt gccgttgata agtgctttta
     1381 ccggctccag accaagatag gttccgtttt caatggcttt gagcacagca cctccaccgg
     1441 tgaaaatata gtatttcgga ctgtcgagtg ccataatata caacccgggt aaaagtcgtt
     1501 tcagttcctg cattgtgtcg ccaccgccgt agagcttaac cgcattgata ttggtatcaa
     1561 ttaggctgtc gagagcaatt gtaccatcgt tgaagtgtgg ggtgtagccc ataactgcat
     1621 ttacaaaaat tgtatttgct ttattgaaaa cctctttcac gtgagcgtct tcaaacgatt
     1681 cgggagcaat gtcgagtacg taattcaact ttgctccgct ttttaatttc cgaatgtcgt
     1741 gaaccctgtt tttcccttca attttccctt caaacacgtc tgattcaaca atataaggca
     1801 attcaacaat gcgatcggcg tatttttccg attttgtaat aaactctttt gccaaccgga
     1861 tgtcgtcgtc ttcgatgccg gtgatcgaaa ttccgtattt tgctgccaga taagcattat
     1921 aaattacacc accaagaacc agataatcgg ctttctgaat cagtgcattc agtgaatcga
     1981 tttttgtgtc gaatttgctt ccggcaacaa ccgctaccat aggtttctgt gcatcgtaaa
     2041 tccggtcgag gctttcaatc tctttttgca gtagaaatcc agcgtacgaa ggcaggtatt
     2101 tgcttattcc tacggtggaa acatgggcct gccacgaacc aaacgcatcg ttcacataaa
     2161 tatcggccag accagccatt tgattggcca gacgctctgc cgattcatcc tttgcttctt
     2221 ctccagtaaa ccaccgggtg ttgggaagat atatcatatc gatctcgtgg ttgcgtaatt
     2281 gccgtatcag gtgatttacg ctggtttcga ttcctgcata accggtatcg ccatgaacat
     2341 agaattcagg cacttttatt ttgacatgca atttgttctg aagatattca acaataggct
     2401 gaacagaact ctcttcatca atttttatgg ctccggtttt tttatctttt ggcctgccta
     2461 cgtgggtcat tagaattatt ttaccgcctt tagccaaaat atggaacaat gtcccgaatg
     2521 tggcatcaat acggtaagga tcgtgaatct gacctttttt aacaacgtta tggtcaaccc
     2581 tgacaagtac tactttgtct ttcaaatcag catcctgcaa taaagggagt agtgttttca
     2641 tgtgaaatca atttattcca aatttataac aatattttta aaataaatat atgtttttta
     2701 cacctattca ttacatttgg aaagcttatg agtatcaggc tgccatatat ctcggtcgaa
     2761 gtgaacgaaa actgcaatca atcgtgttgt ttttgctata ataataaggt atcgcaatcg
     2821 gaatatcacc aaagcaacta tgcccagtta cgcaggttgc tggtgaccct ttataaacaa
     2881 gcgagaccag agcgtgtagt atttaccgga ggagaacccc tgcttgaacc aagaattgca
     2941 gaaattgtac ttttctgtaa aatgcgaaaa tcagcagtaa gcatcattac taatggaacc
     3001 gctgcaacga aaaatgtata taaagacctg atattattgc agaccgattt gtttcagttg
     3061 ccggtacaca gctttaatgc tgaagctcac gataagatga caggactgaa aggcagttgg
     3121 gagaaaagtg ttaactcaat cttacaaatc aaatcactag ggggtagggt tgttcctgtg
     3181 attgtgatta cacgacacaa tgtgaaagaa atcgataaaa ctgtacaatt cataatttct
     3241 ttgggaatca aggagctgat gctgaaccgg ttcaatttta gcgaaaacgc cggtaaagaa
     3301 gccgctggta tgtcagcttc aaaaagtgaa cttaatcaag cctatacggc agtaaataca
     3361 ttggcgcgtg ataatgcaat gaaagtttca tcaaacgtga atacgccata ttgtgtttta
     3421 cgaccttccg aatatccatt catccggttt agcggttgcg cttctgatat tttgcggaat
     3481 ccaatcacca cagatattca tgggaacctg cgtaaatgca accatagcaa tgttgttgcc
     3541 ggcaatatat ttcgtacatc tatcgaagaa atcctgcgat ccgactactt gaaaagctgg
     3601 aatgaaataa ttccggattt ttgtaaaaaa tgcagcgact ggccccaatg ccgtggaggc
     3661 tgtcgtgctg ctgcagagcg gacaggtggc aatatctcgc taccagaccc tatggtacta
     3721 ctattatcag aaaatcagca gcttgtttaa ataaccggta attgccattt taaatgaata
     3781 tcagataaat ctgatagaaa gattgattaa atttgtttat ttaaatcact ggtttagtct
     3841 catatttcac aaaatgaaga aaatcaatgg gttatttgct ttgatgtttt gttgcctgat
     3901 ggcgcattct caggaagcaa atctgatact acccgaaggc catttggaaa aaattatcca
     3961 gattgggtac agtcccgatg gaaattatct cgtaagtgcc tccgacgaca acaccatcaa
     4021 agtatgggat gtgagaacag gccgcttact tcataacctt tctgaccata ctgacgatat
     4081 tttgaaattt gtttttattg aaagtgggac caaaatcaga tcgttatcac gcgataaaac
     4141 ggttagaaca tggaacatta aaacaggaga gcttgaattt gtaagtagtg gcagcaaagt
     4201 tgtaaataca cgtgctttta taaatgaaaa aggaactctt gttgccacta ccgatatgaa
     4261 cgacggcatt gcccgcattt gggatgttac tggaaaggaa tgggtgctta aatatgagtt
     4321 acctcttaat gcagaaagtc cattgtattt agtctgtttc agcgataatg acaaatatgt
     4381 ttgcacaaac tatgaggaag gaaaagctgc cgtatgggat ctggaatccg gggaaaagag
     4441 gtttgtaaca aagaataagg gaatcatttt tacagcatct tttagcaacg acaacagaaa
     4501 attaattaca attatctccg acacattatc agtctggaat attgagaccg gtaaattgag
     4561 ttttgaagta aagaagaaca acgggtgtat ttcttgtgca aaatatagca acaacgattc
     4621 aattattatt accggttcta caagaggcga actcaggatt tttgaagggg cgacagggaa
     4681 attaatacga aaaataccag ccaatatcga cgaaatttcc gatatagcga tttcgtccga
     4741 tagtcgattt gtttattctg tcgattacga agggttagtt acgaaatcag acctttcgac
     4801 cggaaagttg attagcgagt tttcaacctt gtatggtagc aatagcacaa tgagatttcg
     4861 gcttcgcctt tcgttaagcc ccgatgaaaa aaccttggca gttaccaatt ttgcaggaac
     4921 agttgaaata tggaacattg ccgacaacag gattcttcat gaactgaagg ggcagacatc
     4981 taagataaaa aaacctgttt ttagtccttt gggcgatatt gttgcagtgg tgagcgctga
     5041 tttcacggtt aaagtttgga attataaaaa tgcaagctta ttacattctc taaagggcca
     5101 tcaaaccccc atcgaaaccc tgacttttac gccagatggg tcaaagatta taactacttc
     5161 cgaagatggc tttctaaaag tgtgggacgt ttcaacaggt cggttgttat ttagtatcga
     5221 ggatttgtat tccagaattg atgatgtgct aataagccct aacggaaaat atctttttat
     5281 tctcccccga tggaaaaatc caagagttta caatattctt tctggcgaat tgctgtttga
     5341 actcatgatt gaatcttcaa aatttagggg tgcatactat tcggatagtg gaagctattt
     5401 gtatgttttt tatcgggaaa acgaccagat taaatatctt gaaataaatg taacaaaaag
     5461 cattgctatt gatgacatcc cagtgggaga aatcgaaaat catgaatttg acggtcataa
     5521 aacggtctta ttgttgcccg ataaaagcgg gttactttac ctggatgaat ttgagttaaa
     5581 ttattgcagt ttaaccgata aaaacaaaaa tgtggttttt agcgcacatc cgatggagat
     5641 aaccaaagat ccttttaaca ataacggtaa attaattgtg ttcgacactt acgatggaac
     5701 cgatgaatta catgcccagg tttgtgatat cgaaactgga aaaacagttt atgatttatc
     5761 cggatataca tttaatccca atggcgggat cgaacagcca ttcagcagcg atgcccgttg
     5821 gatactcacc atggatgact caggttttgt aagggtatgg aacgccgctg acggaaaact
     5881 aaaaaccgaa ataaaatgcg gtgatgtaaa taatatactg gcatttgccc gatttatgcc
     5941 tgacaataat acagttatgg ccggtagttt taaagttaac gaaatgcaat atatctacca
     6001 ctttaatcct gttggaattt atgacatcga aacgggaaat ctcatcggaa caataccttt
     6061 tgagcaagga tttcctgccg cattttgcga tagtgaaaac atcgctgcct attgtaataa
     6121 ctctcagttg gtgttatacg acaccaaaac atgcaaacaa aacgcctcgt atattgctct
     6181 cgataatagc gagtatatag tgattacccc tgaaaaatat tataaatgct caaaaaatgc
     6241 tgcaggaaaa gtactttggc gcgctggtgg taagttttac cgcttcgatc agttcgattt
     6301 gcagtataac cggcctgaca ttgtgctcga aaaagtaggt aatcccgaca cagtattaat
     6361 aaaactttac agggaagcct ataagaaacg cctgagaaaa agcggattca gtgaaaatgt
     6421 atttcaaccg ggttggagct gtccggaggt tgaaatttta aatgccagca cattcccatg
     6481 gataactgat aaatcaaaaa ttacattgaa tatcgttgcg gaagataaaa atgcctcact
     6541 caacaggtta aatatcagtg ttaatgaggt gccgataaat ggtgtaaccg gcatcgggat
     6601 aaaaaccgat aacttgccgt cggtcgagaa ttgggtcgaa gtggaccttt gtgaggggta
     6661 caacattatt gaagtctcct gtaccaatga atttggggtt gagtcattta aagaaaatct
     6721 cgaaattaat tatgtgccgg cggttcctca tcaaccaatc ctgcacctga ttgctgtatc
     6781 ggtatcggaa tatgcggatg ggagttttaa tcttacctat ccggtaaaag acggaaggga
     6841 catggttgct ttattgggga atggaacatc cggaacctca gcaattgccg tggtcgacac
     6901 attgttcaac gcaatggcaa cccgaagcaa tttgcttgat atgaaaactg atcttggcaa
     6961 aacaaatatc gacgatattg tgattttata tctatcaggg catgggttac ttagcgataa
     7021 tcttgatttt tattatgcaa cttacaatgt cgattttgca cgccccgaat tacagggcat
     7081 ttcgtacgac gaactggaat ggctactcga cggtattccg gcacgtaaaa aattaatact
     7141 aatcgatgcc tgccatagcg gagaactcga cagagattat gttgatcaga ccaacgacag
     7201 tgttatactt gccgatggca cacgcagcgg actgaaaact tattcctata aaaccgccat
     7261 gatgccatcg cagcagggaa atataccgtt taactcagca tttgaactca tgcaggaaac
     7321 tttcaacgac ctgagtaaag gttccggcgc tgttgtaatt tctgctgcgt ctggcacggg
     7381 ttttgcaatc gagtcgcccg tgtggaataa cggagttttt acgtatgcag ttatttcagg
     7441 attaaaagag ttgagggctg atttgaacaa tgataatatg attactgttt ctgaattgaa
     7501 agtttttgta agcaacgaag tggaatcgct aaccagagga aggcaaaaac catcgggaag
     7561 gggcgaaagt ttgcttttcg actggaaatt ggtgcagaaa gaatgataaa ctctcaaaac
     7621 aataaataat gatttggttt gcttaaaaac actaaaaaat ctgccgattg tattaataaa
     7681 tatgttattt ttgtaaccta aataaactaa gtttaattta aattctaatt ctatgaaaac
     7741 attacgaatt gcagttgttt taattattgc ggctttctca acctatatgc ttaccagctg
     7801 cgaccctgag agtgctgaac ctacaatcac ttgggaaggt attatttcgt ataactacga
     7861 tgcaaacgaa gatggcaact atgcccacac cttgaatctt gtagttgatg ctgaagcagg
     7921 gataaacctg tttaatatct ggaaacatgt aattaccgac accgacacag tatcgtcgct
     7981 tgttgacagt cctgccgatt gcatcggact gcttacctac aaccacgatc tcgatatcgc
     8041 agttactcct gccgacgttg ccggtggtgt tacaaaaatt atttacgaaa tggaagttac
     8101 tgacaacgac ctgaacacag ctaccgaaga atttgtgatt tttgttacca acggaacatt
     8161 gagctacaat gtaacttttg ttgttaaaga tgctttgggt ggcgacattg ccgatgctat
     8221 catggtattc ggtgattcaa ccaacgctgc tggtgattat acactggttg gcgttcctgc
     8281 aggaacctat aactacactg taaataaagc cggttacgac gaaaaagcag gtacaggttt
     8341 tgtattggct tccgacacaa ctgttaccat tgaattgcag caagagtggt cagccgaagt
     8401 tcctctggct ctttacggac aaaccacttg ggctacttat cagggaaatc ctgtaacggt
     8461 ttattattcc gaagtatttg gtgtagctgt tgattttacc aatgctggta ttttaaggat
     8521 tattgatact aaacatgatg gcacaactct gggttgcgat ggatgggtgg ttgtgaatgc
     8581 tgccgatgtt gctgctatga atacacaggc tgctctggct actgcttatg ctgccggaac
     8641 agtgattcat acctatgatt tacagtgcga tcagcataaa aattatgaag aattgaactt
     8701 catttcgaaa atgggttcta cctacaaaat cgttcgttat gttgccggat accg
//
LOCUS       DAOOQP010000123         8736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_9936_length_8736_cov_5.716738, whole genome shotgun sequence.
ACCESSION   DAOOQP010000123 DAOOQP010000000
VERSION     DAOOQP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_8736_cov_5.716738"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..2909
                     /locus_tag="PLK75_10950"
     CDS             150..2909
                     /locus_tag="PLK75_10950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOY39804.1"
                     /translation="MNRTIIFIILAIGMSALLITIPFFNKINKFQTLPLSEMVNPMVG
                     TDAHGHTFPGAVIPFGMVQLSPDTRLSGWDGCGGYHYSDSVMYGFSHTHLSGTGCSDY
                     GDILIVPMSGFYSMWNVAEATGNTMGRSYTSKFSHATEKASPGYYSVFLETPGVDAEM
                     TVTQRVGFHKYHYRSMGKHFVILDLEHRDKLIETSLKKISDTRYVGLRRSKEWADDQY
                     VYFVIDFSSPVEIVDYRGGLLPDKNNENMPAKLEPIPDAFNSQATVYVFEVADSSVND
                     LMIKTALSTVSTDGAIKNMEAELPDWDFDSVKHNAEELWNAELSKIIVKGGSDEQRKV
                     FYTALYHSFIHPSLAQDVDGQFRGTDLQVHKATEFTNYTVFSLWDTYRAIHPLFTIVQ
                     QKRTLDFIKTFLAQFTYGGQLPMWELSANETGCMIGYHAVPPIVDAYSKGITDFDTHL
                     ALKAMLQRAEADELGKHDYMKYGFLPIESEHESVSKTLEYGFDDWCIARFAKMTGNEE
                     VFKNYLVRSQAYKHIFDTETGFMRPKQNGSWKTPFDPKEVDCNYTEANAWQYSFYVPH
                     DILGLIAMMGGKEMFEAKLDELFSADSKTAGRTQADITGLIGQYAHGNEPSHHIAYLY
                     NYCCKPEKTQKMVRRIMNEMYNSTPSGNCGNEDCGQMSAWYVFSAMGFYPVNPANGVY
                     DFGSPIFDTVVIQLENGKNFTIIAHNNSAKNMYIDRIELNGRDYKYSYLLHKTIMKGG
                     KLEFYMSDKPGNTVGKTPETIYHSVVQEHLVTPVPYTDASRLTFYDSIQIQLSCPVEG
                     AMIYFRCDSTKRFLVYTKPFTISENTVLQTYAKKSGMRSSKTIEAKYIKIEGNRKIKL
                     NSTYDTQYSAGGDDALIDRIKGNKDFRSGTWQGYQGQDFEVVVDLGEIKPVKTIETCF
                     IQELR"
     gene            2973..3170
                     /locus_tag="PLK75_10955"
     CDS             2973..3170
                     /locus_tag="PLK75_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39805.1"
                     /translation="MSKITHNIPDTDYNQQIFNFTYTPENQKTRYIKVFAKYYGQLPE
                     WHLGAGGKSWLFADEIDIICK"
     gene            3219..4022
                     /locus_tag="PLK75_10960"
     CDS             3219..4022
                     /locus_tag="PLK75_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39806.1"
                     /translation="MRTGLSARTYLQFGAIVITTFFIICCGNTVNYNDLKDAFAETEN
                     SSDTAICPDSIFSLIPEYDSFIKPLSVSGRSFNKDVVVGISTQNEELGIKQTCFLLGV
                     YIADIGYVRHYEQVQLCMDYLEEMKSCAARLSIPEELFNDVIVRSEATLTSKEQLFAI
                     ADSVVKNTSDYLEENELYGISVIIAAGLWLETYRIGIQLPLQRNDELMVNHMILLDQL
                     IRATGLVRNDQFIQKLNTSLVQVQDAYNSNMPPEKLLEGIYESLGISVH"
     gene            4129..4884
                     /locus_tag="PLK75_10965"
     CDS             4129..4884
                     /locus_tag="PLK75_10965"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOY39807.1"
                     /translation="MEIKKEYRFTCSQNKKSCSGIKKVTFGLLLVAAGVLLILDRTGL
                     IKHELMQYIFSWQSLLIALGIWGLSGFRAIAFHLMLIATGGFFLYAEIVKLPIETQHI
                     FWPVVLVFVGLIIIFKKFNIDRHRKRMNENTEISSQDFIEKKILLSGDNSIITSKNFQ
                     GGNISAVLGGCELNMEQAEMAEGNHILNISAVLGGINIIVPKHWDVIVEVDGVLGGFK
                     DERKFVNTELIDRSRRLFIRGSAVMGGGEVKYS"
     gene            4885..5937
                     /locus_tag="PLK75_10970"
     CDS             4885..5937
                     /locus_tag="PLK75_10970"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY39808.1"
                     /translation="MNHPLLKYRKYLWTYIASWLLLTVANFLFMFAVNNVGVTESVID
                     AVIFNTLFAVFGFAIYYVTYYAGLDKRFGYIVLNHFFSALIFVGLWLLISALALNLIY
                     SILGLETLDSPAWLQFKFLQGLFYYVVMALIYYLMRTSEVNRQAKTDLLQLENELKEA
                     QLQTLKSQINPHFLFNSLNSISYLVSTDSTKAREMLSVLSDYFRYSLKNQSKLSSIDD
                     ELIHCRKYLEIEQVRFGEKLLIVENIDRSISHLLVPSMILQPLYENAVKHGVYESRTP
                     VTIATHLSDHDDFVKLIIENNYDEESIPRKGEGIGLKNVMQRLEITYGHPNLVTIIRK
                     PDKFVVEIHIPKNIAP"
     gene            5934..6677
                     /locus_tag="PLK75_10975"
     CDS             5934..6677
                     /locus_tag="PLK75_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOY39809.1"
                     /translation="MIPCIIIDDETPARSIIKEFLNDHKDFYVIGEASNGFDGFKMIQ
                     DLKPIVVFLDIQMPKLTGFEMLELLDEKPIILFVTAYDQYAIKAFECNAVDYLLKPFT
                     PNRFSEALIKIRVRIAAAKSDNMQFNALVNSYKSELSCIDRVVVKSGNKIKILPLHQI
                     VCIEAADDYVIIHTGDEQFIKNESLKFYEAKLPEKEFLRVHRSFIISLSYIDSIEPYT
                     KETHLIKMKNGLSVKSSRTGSHLLKAILH"
     gene            complement(6674..8095)
                     /locus_tag="PLK75_10980"
     CDS             complement(6674..8095)
                     /locus_tag="PLK75_10980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY39810.1"
                     /translation="MISDLIILIVCLTGYAYAFRVQQKNRHLLALSMLVLLGMILRTY
                     LSLDLFLHPWDERYHALVAKNMLQSPFSPMLYNDPIMPFDYRDWSANHIWLHKQPLPL
                     WLMSVGVWLFGNTEFAIRVPSILFSGVGIAFMYLLARELYDKKVAFLSAFLFSIHGLI
                     LEITAGRVATDHVDLFFLVFVLAAVYFSVKSYKTGKLSYSIFCGFSIGLAILCKWLPA
                     LIVFGIWGILVFKKWPFWKMLGHLSVMLLITTVVALPWQIYIHLRYPAEALWENGFNV
                     KHITEVLDGNGGPLYYHFDKLRMIYGELIYLPILWLTYKIIRRKRNRTDIAVFLWFLI
                     PFVFFSIPKTKMQAYTLFAAPAIFLIQSLFIVYLFRYRKVLKPKILTYTILFLLMALP
                     VRYSTERMKFFTAYERQPQWATDIKTLKKSLPENTHNLVIFNTPHPIETMFYIDCIAY
                     DFVPDSTLINNIKDRGYTVKIMN"
     gene            8229..>8736
                     /locus_tag="PLK75_10985"
     CDS             8229..>8736
                     /locus_tag="PLK75_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39811.1"
                     /translation="MKSFLGIQVMLLLCLSSMAQVYQSYMNDMGIIDNFEVPYLDNLS
                     KSGDTLPQLSGWPKGFVRNPSFQNFRGLTLEYLDDVPGHEIIAGINDSLYVLHGDGSR
                     LWSVKLDGTAIYPASTADIDNDGEMDIVIVTGGVPYNGHVYVFDNQGNIKTGWPQSVA
                     SCWYICAPV"
BASE COUNT         2746 a         1569 c         1822 g         2599 t
ORIGIN      
        1 cgtcaaagaa caacctacac tatttgatta aaaaattaac gcaccactac taaattcaaa
       61 acaattttac tagtgctgta ataatcttgg aaaagaaaaa ttttcatttt gattgttgaa
      121 tcacataggt tatttttact aaaaaaaata tgaatcgaac tattattttc attattctgg
      181 ctattggtat gtcagcatta ctgataacca tacccttttt taataaaatc aacaaattcc
      241 aaacactccc actttctgaa atggtgaacc caatggtggg aaccgatgcc cacgggcata
      301 cttttccggg agcagtaatt ccctttggaa tggtacagct tagtcccgac acacggttaa
      361 gcggttggga tggatgtggc gggtatcatt actccgattc ggtaatgtac gggttttcgc
      421 acacgcatct tagtggtaca ggttgttctg attatggcga tattctcatt gtgcctatgt
      481 cgggttttta ctcaatgtgg aatgtagccg aagctacagg gaataccatg ggcagatctt
      541 atacatccaa gttctcacac gcaactgaaa aagcgtcgcc cgggtattat tccgtttttc
      601 tcgaaacacc gggtgtagat gcagaaatga ctgttaccca aagagtaggc tttcataagt
      661 atcattatcg cagtatggga aagcattttg ttatactcga ccttgaacac cgcgataaac
      721 tgattgaaac cagtttgaaa aaaatcagtg atacgcggta cgttggtctc cgtaggtcga
      781 aagagtgggc cgatgatcag tatgtgtatt tcgtaatcga tttttcttca ccggtagaaa
      841 ttgtcgacta tcgtggcggt ttactgcctg ataaaaacaa tgagaatatg ccggctaaat
      901 tagaacctat tcctgacgct tttaactcac aggcgactgt ttatgttttt gaagttgctg
      961 acagttcggt gaatgatctg atgataaaaa cagccttatc gacagtaagt actgatggtg
     1021 ctattaaaaa tatggaagca gaattgcctg actgggattt cgactcggtt aagcataatg
     1081 cagaagaatt gtggaatgct gaactcagta aaattattgt aaaaggaggc agcgatgagc
     1141 agcggaaagt tttttacact gcgctttatc acagttttat tcacccaagc ctggctcagg
     1201 atgttgatgg acagttcagg ggcactgacc tgcaggtgca caaagcgaca gaatttacaa
     1261 attatactgt gttttcgtta tgggacactt acagggctat tcatccgctt tttaccatag
     1321 tgcaacaaaa acgtacactc gattttatta aaacatttct ggcacaattt acctatggcg
     1381 gtcagcttcc tatgtgggag ctttcggcaa atgaaacagg gtgtatgatt ggatatcatg
     1441 cagttccacc cattgttgac gcatatagca aaggcataac agatttcgat acacatctgg
     1501 cacttaaggc gatgttgcag cgtgctgaag ctgatgaact gggaaagcac gactacatga
     1561 aatatggatt cttacccata gagtcggagc atgaatctgt ttcgaaaaca ctcgaatacg
     1621 gtttcgatga ttggtgtatt gctagatttg cgaaaatgac gggtaatgaa gaagttttca
     1681 aaaattacct tgtgcgcagt caggcataca aacatatttt cgatactgaa accggtttta
     1741 tgagacctaa acagaacgga agctggaaaa ccccttttga ccccaaggaa gttgactgca
     1801 attataccga agccaatgca tggcaataca gtttttatgt accacacgat attttgggtt
     1861 taatcgcgat gatgggaggt aaagaaatgt tcgaagccaa acttgatgaa ctgttttcgg
     1921 cagacagtaa aacagcgggt cgcacacagg ctgatattac cggacttatc ggacaatatg
     1981 cccacggaaa tgaacccagt catcatattg catatctcta caactattgc tgcaaaccgg
     2041 agaaaactca aaaaatggta cgccgcatta tgaatgaaat gtacaattcc acgccgtcgg
     2101 gtaattgcgg aaacgaagac tgtgggcaaa tgtcggcttg gtatgtattc agtgcaatgg
     2161 gcttttatcc tgtaaatccg gcaaacggtg tatacgattt tggaagtcca attttcgata
     2221 cggttgttat tcagcttgaa aacggaaaaa acttcaccat aattgcccat aacaatagtg
     2281 caaagaatat gtacatcgat cgtattgagc tgaatggcag agattacaaa tattcatacc
     2341 ttttacataa aaccattatg aaaggaggta aacttgaatt ttatatgagc gataagcctg
     2401 gtaatactgt tgggaaaacc cctgaaacaa tatatcattc agtcgttcag gagcatttgg
     2461 taactccggt tccttacacc gatgcctcaa gactgacatt ttatgattca attcaaattc
     2521 aattaagttg ccccgttgaa ggtgcaatga tatatttcag atgcgattct acaaagcgat
     2581 ttcttgttta cacaaaacct ttcacaatta gcgaaaacac tgtgctgcaa acctatgcta
     2641 aaaaaagcgg aatgaggagc tccaaaacca ttgaagcgaa atatattaaa attgaaggta
     2701 atcggaagat aaaactaaat agtacttacg atacgcaata tagtgcagga ggtgatgatg
     2761 ctcttatcga cagaattaag ggtaacaaag attttcgttc cggcacatgg cagggctatc
     2821 aggggcagga tttcgaagtt gttgtcgacc ttggagagat taaacctgtg aaaacaatag
     2881 aaacctgctt tattcaggaa ttgaggtaat ggattttact tcctttgtat gttgaatact
     2941 atttctcggt tgatgggaca aactttgtga agatgagtaa aattacccat aatatacccg
     3001 atacggatta taatcagcaa atttttaact ttacctatac tcctgaaaac caaaaaacac
     3061 gatatattaa ggtttttgct aagtactatg gccaattacc tgaatggcat ttgggtgccg
     3121 gaggtaagtc gtggcttttt gctgacgaaa tcgatataat ttgtaaatag aatgtttgcc
     3181 cattgaagaa tagcttttta aaatttagat ctccccaatt gaggactggt ctttctgcaa
     3241 gaacttattt gcagtttgga gctattgtta ttactacatt tttcattata tgctgcggaa
     3301 atacagttaa ctataacgac ctgaaagatg catttgctga aacagaaaac tctagcgata
     3361 cagctatttg tcccgatagc attttttcac ttataccgga gtatgactcg ttcataaagc
     3421 ctctttcagt atcaggtaga tcttttaata aagatgtcgt tgttggtata tcaacacaaa
     3481 atgaggaatt gggcattaag caaacttgtt ttttattagg ggtatatatt gccgatatcg
     3541 gatatgtacg acattacgaa caagtacagc tttgcatgga ttaccttgaa gaaatgaaat
     3601 catgcgctgc aaggctttcg atacccgaag agttatttaa cgatgttatt gtcaggtctg
     3661 aagcaaccct taccagtaaa gagcagcttt ttgctattgc tgatagtgtt gtaaaaaaca
     3721 cgtcagacta tctggaggaa aatgaattgt atggaatttc ggttataatt gctgctggac
     3781 tttggctcga aacttaccgc attggaattc agcttccgtt acaacgtaac gatgagttaa
     3841 tggtgaatca tatgatattg cttgatcagt tgattcgggc aacaggtttg gtgaggaacg
     3901 atcagtttat tcagaaattg aacacaagcc ttgtccaggt gcaggatgcg tataattcta
     3961 atatgccacc ggaaaagttg ttggaaggga tatatgaaag cttgggaatt tcggtgcact
     4021 aggtaccaag attcgttatt taccgatgag aatcgtaatt tgccgtatcc ggtttttaaa
     4081 agggggattg ccgctgtagt tttgactcaa atttaaaagc aaattgtcat ggaaataaaa
     4141 aaagaatatc ggtttacctg ttcacaaaat aaaaaatcgt gctcaggtat taaaaaagtt
     4201 actttcggat tgttgttggt tgctgctggt gtgcttctga ttctggaccg cacgggcttg
     4261 attaaacatg aattgatgca gtacattttt agttggcagt ctcttcttat tgcacttgga
     4321 atctggggct tatcgggatt tagagcaatt gcattccacc ttatgcttat tgcaaccggg
     4381 ggattttttc tgtatgccga aatagtaaaa cttcccattg aaacgcaaca tatattctgg
     4441 ccagttgtgc tggtttttgt gggattaata attattttta aaaaatttaa tatcgaccgg
     4501 cacagaaaac gtatgaacga aaacacagaa ataagttcgc aggactttat tgaaaaaaaa
     4561 attctgctga gtggcgataa cagcataata acttcaaaaa actttcaggg tggaaacatt
     4621 tcagccgttc ttggaggctg cgaattgaat atggaacagg ccgaaatggc agaaggcaat
     4681 cacatcctga atatttcagc agtattggga ggtattaaca taattgttcc caaacattgg
     4741 gatgtaattg ttgaagtgga tggtgtactt ggcggtttca aagatgaacg aaagttcgtt
     4801 aatactgaat taatcgacag gagtcgtcgc ttgtttataa gaggaagtgc agtaatgggc
     4861 gggggagaag tgaaatattc ataaatgaat cacccactac tgaaatatcg aaagtatttg
     4921 tggacatata tagcaagttg gctgttactt acagtagcca actttctttt tatgtttgca
     4981 gtaaataatg tgggtgtcac tgagtcggta atcgatgcgg taatatttaa tacattattt
     5041 gccgtatttg ggttcgcaat ttactatgtt acctattacg ccggtttaga taagcgattt
     5101 gggtacattg tgctgaatca ctttttttca gctttaattt ttgtaggcct ttggttgctt
     5161 atttcggcac tggcgctaaa cctgatttat tcgattctgg gtttagaaac attagactct
     5221 cctgcttggc ttcagtttaa gttcctgcaa ggacttttct attatgtggt tatggctctg
     5281 atttattatt tgatgaggac gtcggaagta aaccggcagg caaaaaccga tttactgcaa
     5341 ctggagaacg aacttaagga agcacaactt caaacattga agtcgcaaat aaacccacat
     5401 ttcttattca atagtttaaa ctcaatttcg tatctggtaa gcaccgacag cactaaagcc
     5461 cgcgaaatgc tttctgtatt atccgattat ttcagatata gtcttaaaaa tcaatcaaaa
     5521 ctatccagca tcgacgacga attgatccat tgccggaaat atcttgaaat tgagcaggtt
     5581 cgatttggag aaaaactact gattgtggaa aatattgatc gcagtatctc acacctgctt
     5641 gttccttcaa tgatattgca acctttgtac gaaaatgccg ttaaacatgg agtttacgaa
     5701 agcaggactc ctgttaccat tgcaacacat ctgtccgatc atgatgattt tgttaagtta
     5761 attattgaaa acaattacga cgaggagtcc attccaagaa aaggggaggg gattggcctt
     5821 aaaaacgtaa tgcaaagact cgaaatcact tatggccatc ccaatctggt taccattatc
     5881 agaaagcccg ataagtttgt tgttgaaatt catattccta aaaacatagc accatgatac
     5941 catgtataat tattgatgat gagacccctg cacgtagcat tattaaagaa tttctgaacg
     6001 accacaaaga tttttatgtt attggcgaag cttcaaatgg tttcgatggg tttaagatga
     6061 tacaagacct gaagcccatt gtggtttttc tggatataca aatgccgaaa cttaccggct
     6121 tcgaaatgct cgagcttttg gatgaaaagc caattatact ttttgttact gcatacgatc
     6181 aatatgctat taaagctttt gaatgtaatg ccgtcgatta tttacttaaa ccatttactc
     6241 cgaacaggtt tagtgaggct cttattaaaa ttagggtgag aatagccgct gcaaaatcag
     6301 ataatatgca atttaatgca ctggttaatt cttataaaag cgaattgagc tgtatcgaca
     6361 gggttgttgt gaagtcagga aataaaatca aaattcttcc tttacatcag attgtgtgca
     6421 tcgaagcagc cgacgattac gtgattattc atactggaga tgaacaattt attaaaaatg
     6481 agtcacttaa attctatgaa gcaaaactcc ctgaaaagga gtttttgcgc gtacacagat
     6541 cgtttattat ttcgttaagc tacatcgata gcattgaacc atatacaaaa gaaactcatc
     6601 ttattaaaat gaaaaacggt ttaagcgtaa aatcgagccg gaccggatca catctgctaa
     6661 aagcaatttt gcattaattc attattttca ctgtgtaacc tcgatctttg atgttgttaa
     6721 tcaatgtgct gtcaggcaca aaatcataag caatgcaatc gatataaaac atggtttcaa
     6781 taggatgagg tgtattgaat attaccagat tgtgggtgtt ctccggaaga gatttcttca
     6841 aagttttaat atctgtagcc cattggggct gacgttcata tgctgtaaag aacttcattc
     6901 tttcagtgct gtaacgtaca ggcaaagcca tgagcagaaa caaaattgtg tatgtcagga
     6961 ttttgggttt gagcaccttg cgatagcgaa acaggtaaac tataaacaag gactgaatca
     7021 gaaaaattgc cggagcagca aacaatgtat atgcctgcat ttttgtttta ggaatcgaaa
     7081 aaaacacaaa cggaattaag aaccacagaa aaactgcgat atctgtccgg tttcttttac
     7141 gtcgaataat tttataggtg agccataata taggcaggta aatcaactct ccgtatatca
     7201 ttcgtagttt gtcgaaatga taatataacg ggccaccgtt gccatcgagc acttcggtta
     7261 tatgttttac attgaagcca ttttcccata atgcctcagc cgggtagcgc aagtgtatgt
     7321 atatctgcca tggcagtgct acaacagttg taattaatag cataaccgac aagtgcccga
     7381 gcattttcca aaacggccat tttttgaaaa ccaaaatacc ccatattccg aatacaatca
     7441 gtgcaggaag ccatttgcat aatatggcca aacctataga aaatccacaa aaaatactgt
     7501 acgaaagttt tccggtttta taagatttca ccgaaaaata gactgcagcc agaacaaaaa
     7561 ccagaaaaaa cagatccaca tgatcggtag caactctccc agctgtgatt tcgagaatca
     7621 gaccatgtat cgaaaataaa aaagccgata aaaaagctac tttcttatca taaagttccc
     7681 ttgcaagcaa gtacataaat gcaatgccaa caccagaaaa cagaattgac ggaaccctaa
     7741 ttgcaaattc tgtgttcccg aacagccaaa ctccaaccga cattaaccaa agaggaagag
     7801 gttgtttatg caaccatata tggtttgcgc tccagtcgcg gtaatcgaat ggcataatgg
     7861 ggtcgttata taacatcgga ctgaaggggc tttgaagcat gttttttgca acgagtgcat
     7921 gataacgctc gtcccagggg tggagaaaca aatcaagact gaggtaagtg cgcagaatca
     7981 tcccgagcag cacaagcatt gataatgcaa gtaaatgcct gtttttctgt tgaacccgga
     8041 atgcataggc atagccggtc aggcaaacaa ttaatataat taggtcggaa atcatggtgc
     8101 caattcaaaa aattgattta caaattaaag aagaaaacaa taaaaaacgt tcttttaggc
     8161 ttcagatagc tctgtttttt tctaaatttg ctttgtcctc actattttat catgtaaagt
     8221 tttataaaat gaaaagcttt ctgggaatac aagtaatgct gcttctttgc cttagctcaa
     8281 tggcacaggt gtaccaatca tatatgaatg atatgggtat aatagataat tttgaagtgc
     8341 catacctcga taatctttct aaatcgggtg acactttgcc ccagttatct gggtggccaa
     8401 aagggtttgt caggaatcct tcttttcaga atttcagggg attaacactt gaatatcttg
     8461 atgatgttcc ggggcatgaa attatagccg gtatcaacga ttcgctttat gtgctacatg
     8521 gcgatggctc tcgtttatgg tccgtaaaac tcgacggcac cgcgatttac ccggcttcaa
     8581 ctgccgacat tgataatgat ggcgaaatgg atattgtgat tgtaaccggt ggagtgccat
     8641 ataatgggca tgtttatgtg tttgataatc agggtaatat caagactgga tggccacaga
     8701 gtgtagcatc gtgctggtat atttgtgctc ctgttt
//
LOCUS       DAOOQP010000124         8596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_10158_length_8596_cov_5.797213, whole genome shotgun sequence.
ACCESSION   DAOOQP010000124 DAOOQP010000000
VERSION     DAOOQP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_8596_cov_5.797213"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLK75_10990"
     CDS             complement(<1..283)
                     /locus_tag="PLK75_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOY39812.1"
                     /translation="MRIALLISVICITGNLFSQNVTIAQNFSITDTDGNTYVLYDELD
                     ANKTVYLFFFSVDCGHCHIQAPNVDSVWQQLGSGTGEVLVWGFESSTLEP"
     gene            complement(382..1980)
                     /locus_tag="PLK75_10995"
     CDS             complement(382..1980)
                     /locus_tag="PLK75_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39813.1"
                     /translation="MKKLFWPLIPFLFFAVSGFTQALSVNFGELEYTIPRGHDYVKMV
                     GGDNQSIFAVRMDEKNQLYLDQYNSSSMQQISANPITLPIIGDVQSKFVELIFLDGKL
                     ILFTKVVKRSNNIADNTNTDVLYAHYIDETGKVDENSQTILSDPEDPMMDVDYSVELS
                     TDGQYIFIHYNRKFTKYAQEKFYLKVIGSDLEKRFGKKITLPLDNQVFNIIQYETNKL
                     GYVYMMAEITPEKRSKRTNVSTTSQFKLLVYNVKEDKTSAYDVKAEKFELANVLMGVD
                     FKGNVDIYGLMSRKGKNELEGFFHKKFDIEGGKFLAADAKKAYYVFKREELPDFRNER
                     VSERYDQIYNYALKDILYLGNDESIVIAEHQNYWVDSTPDPQTKTTNYYEYMKYNDLL
                     IANTNAQNNMVWMKRIPKSQVSYNDFGKYSSYYAAANSNGLWAFLFYNDNPKNIKALN
                     KKVLDGEKYKSCFLPNRSGSPVVVCIASDGDIIGYPMFTKKLQKNIIVPSMIARYGDY
                     FYTYTQKSIKYKFASFTISLQSFD"
     gene            complement(2099..2782)
                     /locus_tag="PLK75_11000"
     CDS             complement(2099..2782)
                     /locus_tag="PLK75_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY39814.1"
                     /translation="MQKEINFYQLLSNTPFFGNLDETSVMKLLASVHYQLKSYNKGDI
                     IVHKNDVCEWFMLVTSGSVRGEMSDPEGKVLKIENVMAGHTIALAFLFGRNARYPVTV
                     VANENSQIFRILKQDLIKMLQKSRELLETMLILVSERTQFLTGRIHFLSLKTIKGKLS
                     FYILQRVKPNSQFVYFERNQTELSEFFGVARPSLARALKQLEEDGCIEVKGKSVKILN
                     LQLLKSLSH"
     gene            2873..3757
                     /locus_tag="PLK75_11005"
     CDS             2873..3757
                     /locus_tag="PLK75_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY39815.1"
                     /translation="MKRKIIRIDEELCNGCGNCVPNCHEGALQIIDGKARLISDLMCD
                     GLGACLGHCPEGAITVEEREAEEYNETEVMKGMIPNGKNTLIAHFTHLLDHEQDEYFK
                     EGISYLFDRKDELAFDVQEVIEGIRNHVDSKSCGCSGHSNHPGGCPGSQEQSFEKNTK
                     STDLISSDSQLTHWPIQLHLINPSSAHFKNCDLLVAADCTAFSIGNFHPDYLKGRKLI
                     IACPKLDDGQDSYLEKIRKLIAVAHVNTITVMRMEVPCCFSLVQMVKHAIDETGAKVP
                     LKEIVVGTTGEIVSSNWI"
     gene            3802..5445
                     /gene="hcp"
                     /locus_tag="PLK75_11010"
                     /gene_synonym="priS"
     CDS             3802..5445
                     /gene="hcp"
                     /locus_tag="PLK75_11010"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOY39816.1"
                     /translation="MFCYQCQETAKGTGCTVAGVCGKTEEVANMQDLLIYVTKGISIY
                     ATIAREKGIENHEVNKFVFDALFMTITNANFDKAAIIAKIKKGFEIRDLIKAEILKAG
                     IKLPENMHSSTGWFSTDEAEMEHKNKLVGVLSIGNEDIRSLKELLTYGIKGMAAYAEH
                     AFNLGYENPGVFAFMQKGLVATTEEKTVPELIDLVMECGKFGVDVMALLDKANTETFG
                     NPEITEVNIGVRNNPGILISGHDLNDMEQLLKQTEGTGVDVYTHSEMLPANYYPAFKK
                     YKHFVGNYGNAWWKQREEFETFNGPILFTTNCIVPPHKNAGYLGKIYTTGAAGLEGAK
                     HIPTPPKGQNKDFSEIIAHAKKCQPPVEIEKGKIIGGFAHNQVFQLADKVVDAVKSGA
                     IRKFFVMAGCDGRMKSRDYYTQFAGKLPKDTVILTAGCAKYRYNKLELGDIGGIPRVL
                     DAGQCNDSYSLAVIALKLKEVFELNDVNELPIVYNIAWYEQKAVIVLLALLYLGVKNI
                     HLGPTLPGFLSPNVANVLVQNFGISGVSEVEKDIELMMA"
     gene            5498..5677
                     /locus_tag="PLK75_11015"
     CDS             5498..5677
                     /locus_tag="PLK75_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOY39817.1"
                     /translation="MEKNKIICTCNQVTYGEIEDAVLNHGCDSVEKVQEKTEAGTVCG
                     GCIEDIEEIIKKLKG"
     gene            5682..6011
                     /locus_tag="PLK75_11020"
     CDS             5682..6011
                     /locus_tag="PLK75_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOY39818.1"
                     /translation="MEATDFPRAEVMQLKSMVEYQQGAIVSRTIIKKLQGNLSLFAFD
                     KGESLSEHTAPFDAMIQVLDGSAEVIIGGKPYRVSCCETIIMPANIPHAVNAVEKFKM
                     LLTMIKE"
     gene            complement(6116..7276)
                     /locus_tag="PLK75_11025"
     CDS             complement(6116..7276)
                     /locus_tag="PLK75_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY39819.1"
                     /translation="MSQMIYLDNSATSFPKPDVVYDFMCSFYKKHGVNPGRSGFDAAI
                     ETEEVVFNTRKMLAEFFNGDDPNRLTFSYNASDSLNMVIQGLAEKGDHVITTYLEHNS
                     VLRPLYHLQMNGIIDVTHVPFDKNGFVNPDDIKKAIRKNTKFVIVNHCSNVIGTIQPV
                     AEIGKICRQAGVYFIIDASQSAGAIPIDIKAMNIDIVVFTGHKCLMGPTGIGGSYVQE
                     NVNIHGTRFGGTGVRSAQLTHLEEFPYRMECGTLNLVGVAGIHAGLKWVLDQGMENIH
                     KQEMQLWEKLRLGVNAIPGITTYCANSMVNHNPVLSLNINGFESGDVGIMLDVDYNIA
                     CRTGLQCAPKVHEGIGTDKIHGTVRLSIGAFNTEEEIDTALDALKEIAAIKN"
     gene            complement(7495..8487)
                     /locus_tag="PLK75_11030"
     CDS             complement(7495..8487)
                     /locus_tag="PLK75_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HOY39820.1"
                     /translation="MAASEIKIYQTDDGQTKIDVKFDKETVWLTQKQMALLFDKDSDT
                     IGLHLKNIYQSGELEELSTTEESSVVQQEGKRQVNRKIKLYNLDAIISVGYRVNSKRG
                     IQFRIWASKLIKEYLLKGFVVDNKRLIQQNNQLKELQTSVKILADSMKYKQLTNDERI
                     GLLRIISDYAYALDILDQYDYQNLEIKNTSGKELYQLTYDEAMTQIKLAKKFHGNSDL
                     FGHEKDQSFQSSLSTIYQTFDGVDLYPSIEEKAANLLYFITKNHSFSDGNKRIAAFLF
                     LLFLERNGVLFNDDGNKRIADNTLVALTLMIAVSKPEEKDTMIKVIVNLINKNN"
BASE COUNT         2623 a         1654 c         1678 g         2641 t
ORIGIN      
        1 aaggttcaag tgtgcttgat tcaaatcccc ataccagcac ttcgcctgtt ccacttccga
       61 gttgttgcca aaccgagtcg acatttggag cctggatatg acaatgcccg caatcgacgc
      121 tgaaaaagaa aagataaacg gttttatttg catcaagctc atcatataat acatatgtat
      181 tgccgtcggt atcggtaatt gaaaagttct gtgcaatagt aacattttgg gaaaacaaat
      241 ttcccgttat gcaaataact gatataagta aagctattct cattttaatt acgatgtgca
      301 atttacataa aatttgcaag cagatgttga tgcatatttt ataaaaaacg cagcggttaa
      361 agctgcgtta atgatagtgg gctaatcgaa gctttgcaac gaaatagtga aagaggcgaa
      421 tttatacttg atactctttt gtgtgtaagt atagaagtaa tctccatatc tggcaatcat
      481 cgaaggaaca ataatgtttt tctggagctt cttggtaaac atcggatatc caattatatc
      541 accatcagaa gcaatacaaa caacaaccgg cgaaccagac ctgttgggaa ggaaacaact
      601 cttgtatttt tcgccatcta aaactttctt gttcagggct ttaatgtttt tggggttatc
      661 gttgtaaaac aaaaaggccc ataagccgtt tgaattagca gcagcataat acgatgaata
      721 tttaccgaaa tcattatagc ttacctggct tttcggaatg cgcttcatcc acaccatatt
      781 gttctgggca tttgtatttg ctattaaaag gtcattatac ttcatatatt cgtagtaatt
      841 cgtcgttttt gtttgtggat caggagtgga atcaacccag tagttttgat gctcagcaat
      901 cactatcgat tcatcgttgc ccaaatacag aatatctttt aaggcataat tataaatttg
      961 atcataacgt tccgacaccc tttcgtttct aaaatcgggc aattcttcgc gtttaaaaac
     1021 gtagtatgcc tttttagcat cagccgcaag gaatttacca ccttcaatat cgaacttttt
     1081 gtggaaaaac ccctcgagtt cgtttttacc ttttcgcgac attaatccgt atatatcaac
     1141 attacctttg aaatcgacac ccatcaggac attggcaagt tcgaactttt cagctttcac
     1201 gtcataagcg gaggtcttat cttcttttac attataaaca agcaatttga actgacttgt
     1261 ggttgacaca ttggttcttt tacttctttt ttcaggagtg atttcagcca tcatataaac
     1321 gtaccctaat ttattggtct catattgaat aatattaaag acctgattat ccaatggtaa
     1381 ggttatcttt ttgccaaagc gcttttctaa gtccgaacca attactttta aatagaactt
     1441 ctcctgcgca tattttgtaa attttctatt ataatgaata aaaatatatt gaccatcagt
     1501 gctcaattca acgctgtaat caacatccat catcggatcc tctggatcgc tcaggatagt
     1561 ttgagagttt tcgtcaactt taccagtttc atcgatgtag tgagcataca atacatcagt
     1621 gttggtgttg tcggcaatat tattcgaacg cttaacgact tttgtaaaaa gaattaattt
     1681 gccgtccaga aaaatcagtt caacaaattt ggattgaaca tctccaatta tcggcagcgt
     1741 aatagggttt gccgaaattt gctgcataga gctggagttg tactgatcga gatacagttg
     1801 gtttttttca tccattctta ctgcaaaaat gctctgatta tcccctccga ccatttttac
     1861 gtaatcgtga cccctcggta ttgtatattc gagttctcca aagttaaccg acaatgcctg
     1921 cgtaaatccc gaaacggcaa agaacagaaa aggtattaat ggccaaaata actttttcat
     1981 atgcttattt tttgtttggt tgctaaaata atgaaattcc tgataattac gaaaaaatat
     2041 cttttattgc tctatatatt tattcgtttt aaccgactca agttttgtgc cacatcaatt
     2101 aatgtgaaag cgatttgagc agttgtaaat tcagtatttt tacagatttc cctttcacct
     2161 caatacaccc atcttcttca agctgcttta atgcccttgc cagtgaaggc cttgctactc
     2221 cgaaaaactc cgataattcg gtttggttgc gctcgaagta aacaaattgt gaattgggtt
     2281 ttacgcgctg gagaatataa aatgagagtt ttccctttat tgttttcaaa ctcaggaaat
     2341 gaattcttcc cgtcaggaac tgggttcttt ccgataccag aatcaacatg gtttcgagta
     2401 attcgcggct cttttgcagc atctttatca ggtcttgctt aagaatacga aaaatttgtg
     2461 aattttcgtt tgcaactact gtaacagggt accgtgcatt gcggccaaac aagaaagcca
     2521 gcgcaatggt gtgcccggcc ataacatttt caattttcag caccttaccc tcagggtccg
     2581 acatttctcc tctgacgctc cccgaagtta ccagcataaa ccattcacag acatcgttct
     2641 tgtgcacaat aatatcgcct ttgttgtacg acttcagttg gtagtgaacc gatgcaagta
     2701 atttcattac agaagtctca tcaagatttc cgaaaaaagg agtatttgac aacagctgat
     2761 aaaaattgat ctctttttgc attttttaaa attattttgt attttgtaac aaatgttaca
     2821 aaatatgagc aaataaccat ggatatttgc gatataattt aaaaatagca acatgaaaag
     2881 aaaaataata agaattgatg aagaattatg caatggttgt ggtaattgcg taccgaattg
     2941 tcacgaaggt gctttacaaa taattgatgg gaaagccaga ttgatcagcg atttaatgtg
     3001 tgatgggctg ggtgcttgtc tcggacattg ccccgaagga gctataactg ttgaggagag
     3061 ggaggcagag gaatacaacg aaactgaagt gatgaaaggg atgatcccca atggaaaaaa
     3121 tactcttata gctcatttca cacatttgct cgaccatgag caggatgaat attttaaaga
     3181 gggtatttcc tatcttttcg acaggaagga tgagctggca tttgatgttc aagaggttat
     3241 tgaaggaata cgcaatcatg ttgatagcaa gagctgtggt tgttccggcc attctaacca
     3301 tccgggtggt tgccccggtt cacaggaaca aagctttgaa aaaaatacca aaagcactga
     3361 tctgatatca tctgattctc agcttaccca ttggcctatt cagttgcatc tgataaaccc
     3421 atcttcggca cattttaaaa attgcgacct gcttgtagca gccgattgca ctgctttcag
     3481 cataggcaat ttccatccgg actatctgaa ggggcggaaa ttaatcatag cttgtccgaa
     3541 acttgatgat ggacaggata gttatcttga aaaaattaga aaactcatcg ccgttgctca
     3601 tgtgaatacc attacggtaa tgcgcatgga agtgccatgc tgtttttcac tggtacaaat
     3661 ggtaaaacac gccattgatg aaacgggtgc gaaggttccg ctcaaagaaa tcgtagtagg
     3721 aaccaccgga gaaattgtaa gcagtaactg gatttaaatc atttaagtaa ataataatct
     3781 aaaacctata aaattatgag tatgttttgt tatcaatgtc aggaaaccgc caaaggaaca
     3841 ggttgtactg ttgccggagt atgcggaaaa acagaagaag ttgccaatat gcaggatttg
     3901 ttaatctatg taactaaagg catttcgatc tacgcaacta ttgcccgcga aaaaggcatc
     3961 gaaaaccacg aagtgaacaa gttcgtgttc gatgcattat tcatgacaat caccaatgcc
     4021 aatttcgata aggcagctat aattgcaaaa attaaaaaag gtttcgaaat cagagacctg
     4081 attaaagcag aaattttaaa agcaggtatc aaactcccgg aaaacatgca ttccagcacc
     4141 ggttggttca gtaccgatga agccgagatg gaacacaaaa acaaacttgt tggagtatta
     4201 tccattggga atgaagatat tcgttcgctg aaggaattat tgacatacgg aattaaaggt
     4261 atggctgcct atgccgaaca tgcattcaat ctgggatatg aaaaccccgg tgtttttgct
     4321 tttatgcaga aaggacttgt tgccacaaca gaagaaaaaa ccgttcctga actgatagat
     4381 ttggttatgg aatgcggaaa atttggagtt gatgtaatgg cattgctcga taaagcaaat
     4441 accgaaacct ttggtaaccc cgaaattact gaagtaaata taggagttcg taataatccc
     4501 ggcatcctca tcagcggtca cgatttgaac gatatggaac aattgctgaa acagaccgaa
     4561 ggaaccggtg tagatgttta cacacacagc gaaatgctgc ctgccaatta ttatcccgct
     4621 ttcaagaaat acaaacattt tgttggtaac tatggaaatg catggtggaa acaacgtgaa
     4681 gaattcgaaa cattcaatgg tccgattctt tttaccacca actgtattgt tccgccgcat
     4741 aaaaatgcag gatatcttgg gaagatttat actaccggtg ctgccggact tgaaggtgca
     4801 aaacatatcc caacacctcc aaagggtcaa aataaagatt ttagtgaaat cattgcgcat
     4861 gctaaaaaat gtcaacctcc tgttgaaatt gaaaaaggta aaattatcgg aggttttgca
     4921 cacaatcagg tatttcagct tgccgataag gtggtggatg ccgttaaatc aggtgcaatc
     4981 cggaaatttt ttgtaatggc aggttgcgat ggacgaatga aatcgagaga ttattataca
     5041 cagtttgccg gaaaactgcc caaagatacc gtaatactta cggccggttg tgcaaaatat
     5101 cgttacaaca aactcgaact gggagatatc ggcggaattc ctcgcgtatt ggatgccggt
     5161 cagtgcaacg acagttattc gcttgcagta atagccctga aactgaaaga agttttcgaa
     5221 ctgaacgatg tgaatgaatt gcccattgtt tacaacattg cctggtacga acaaaaggca
     5281 gttattgtgc tgcttgcatt gctgtatctt ggtgtgaaaa acatccacct tggaccaaca
     5341 ttaccaggat tcttatcacc taatgtagcc aatgtactgg tgcaaaattt tgggatatcg
     5401 ggggtttcag aagttgaaaa ggatattgaa cttatgatgg cataatatgt tatatcttag
     5461 gggcggcaac aatgccgccc cttaaaattt atacaatatg gaaaagaata aaattatttg
     5521 cacctgtaat caggtaacct atggcgaaat tgaagatgca gttttaaacc acggctgcga
     5581 ctcagttgaa aaagttcagg agaaaaccga agccggtact gtttgtggcg gatgcatcga
     5641 agatattgaa gaaattatta aaaaactgaa aggttagaac tatggaagcg accgattttc
     5701 cacgggctga agtgatgcag ctcaaaagta tggttgaata ccagcagggt gctattgtga
     5761 gcagaactat cattaagaaa ctgcagggta acctcagttt atttgcattt gataagggtg
     5821 agtcgctgag tgagcacacc gctccgttcg atgctatgat tcaggtactt gacggaagtg
     5881 ccgaagtgat tatcggagga aaaccatacc gtgtttcttg ctgtgaaacg attattatgc
     5941 cggcaaacat cccccatgct gtgaatgcgg tagaaaagtt taaaatgctt cttaccatga
     6001 taaaggaata acttgaaaac aattcatttc atttcattaa taacattggg aaagaaaaag
     6061 taaggatact gatttttaag aggctgtagt tttaaacgac agtgcgcttc agctttcagt
     6121 ttttaatcgc agcaatttct ttgagcgcat caagagctgt gtcgatttct tcttcggtat
     6181 taaaagcgcc tatacttaat cgtaccgtac cgtgaatttt atccgtaccg atgccttcat
     6241 gcacttttgg agcgcattgc aatccggtgc ggcaggcaat attataatca acatcaagca
     6301 taataccgac atcgcccgat tcaaacccat tgatgttcag actaagaacc gggttgtggt
     6361 ttaccatact gtttgcgcaa taggttgtaa tgccagggat agcattgaca ccaaggcgca
     6421 atttttccca caattgcatt tcctgcttgt gaatattttc cataccctgg tcgagtaccc
     6481 atttcagacc ggcatgtatt cctgcaactc ccaccagatt taacgtgcca cattccattc
     6541 tgtaaggaaa ctcctcaaga tgagtaagtt gtgcagatcg cacccctgtg ccaccaaaac
     6601 gggtgccgtg gatatttaca ttttcctgaa catagcttcc accgataccg gtaggaccca
     6661 tcaggcattt gtgacctgtg aaaacgacaa tgtcgatgtt catggcttta atatcgatag
     6721 gaatggctcc tgcactctgg ctggcgtcaa taataaaata cactcctgcc tgacggcata
     6781 ttttaccaat ttcggccacc ggttgaatgg tgcctattac attggagcag tggtttacaa
     6841 taacaaactt agtgttttta cggatggctt ttttaatatc atcaggatta acaaaaccgt
     6901 ttttatcgaa aggtacgtga gttacatcga taatgccatt catttgcaaa tggtacagag
     6961 gacggagcac tgagttgtgc tcaagatagg ttgtaataac atgatcgcct ttttcggcaa
     7021 gtccctgaat taccatattc agcgaatcgc tggcattata actgaatgtt aggcggtttg
     7081 ggtcgtcgcc gttgaagaat tcggcaagca tttttcgggt attaaaaacc acttcttcgg
     7141 tttcgatggc agcatcaaat ccggacctcc cgggattgac cccatgtttt ttataaaagc
     7201 tgcacataaa atcgtaaacc acatcgggct tcggaaagct ggtggccgag ttgtcgagat
     7261 atatcatctg gctcatgttg cgttggttta ttgtgtttta accccgtaaa aatcaatctg
     7321 aattaattac gcaaatctaa aactttatgt tgaagtaaaa aagggttgct gcttgcgaaa
     7381 taattactcg cgaagacgaa attatccagt agtctgagtg gtggttcttg tggtattcga
     7441 agtgtactca ttggcaggag gtaattgggt ggcaggcaac tggcttgcca tgtgctaatt
     7501 attcttatta atcaggttta caataacttt tatcattgta tctttttcct caggcttact
     7561 tactgcaatc attaatgtca atgcaaccaa cgtattatct gcaatgcgtt tgttcccatc
     7621 atcattaaat aatactccgt tacgttctaa aaataataag aacaagaatg ctgcaatgcg
     7681 tttattcccg tcggaaaacg aatgattttt tgttataaaa tacaacaaat ttgctgcttt
     7741 ttcctcaata cttgggtata aatcaacccc atcaaaagtc tgatatatag ttgataaaga
     7801 gctttgaaag gattggtctt tttcatgtcc aaacaaatca ctgttaccat gaaacttttt
     7861 tgccaactta atctgggtca ttgcttcatc atatgtcaat tgataaagct cttttccaga
     7921 ggtgtttttt atctcaaggt tttgataatc atattggtct aaaatatcta acgcatatgc
     7981 ataatccgaa attattctaa gtaatccgat tctttcatca tttgtcaact gtttatactt
     8041 catcgaatca gcaagtattt ttactgaagt ttgaagttct tttagttgat tattttgctg
     8101 aattaatcgc ttgttgtcaa caacaaaacc ctttaataaa tactctttta taagcttgct
     8161 tgcccaaatt cgaaattgta tccctcgttt agaatttact ctatatccaa ccgagataat
     8221 cgcatccaaa ttatatagtt ttatttttct atttacttgt cgtttgccct cttgctgaac
     8281 taccgaggat tcctcggtag ttgaaagttc ctctaactca ccagattgat atatattttt
     8341 gagatgaagt cctattgtgt cagagtcttt atcaaacaac agagccatct gtttctgagt
     8401 taaccaaact gtttccttat caaactttac atcaatttta gtctgcccat catccgtttg
     8461 gtatatttta atctctgatg cagccattta ttctttcttt ttcacaaatc tacaaaaaca
     8521 attataatat ttctctacta ttggtctggc cccctataaa tcggacaatt ttataaacgg
     8581 ttaaacaaag gtacac
//
LOCUS       DAOOQP010000125         8595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_10159_length_8595_cov_5.564169, whole genome shotgun sequence.
ACCESSION   DAOOQP010000125 DAOOQP010000000
VERSION     DAOOQP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_8595_cov_5.564169"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PLK75_11035"
     CDS             complement(<1..1523)
                     /locus_tag="PLK75_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOY39821.1"
                     /translation="MKRAKIPVFNQSFDVRVFIRIFFMSLWLVVLILIASVLAGFLYY
                     RYTLPIYETRSIIQVKEENKTNQFLKLDNEVSQSDLTSVTELIQSKTFLRECFTELPL
                     DISYFKKGTFISSEIYKQSPFTVYVNINNPVIYNTRININFIKDKYQLRYKIGNEDYE
                     YILSPEDWHPIFGGEIFIHYDSPKTIRIEQESDAKSKYFFVINNPGTFLQKISANLIV
                     RVQNDAARTILIMYSDNNALKAAEITNTIAEKFLEYDLSRKKESATNVIKYIDQQLET
                     IYKELDQTEKDLSDFKRENKIPPGLDQYGTNKVTMYTSKVSELEDLQIDIELEIATLE
                     KVKKDISNNPKLNIYELMALVTGSNSERFLTSLLSTLQSLIEEKEELMFDITGNNFRI
                     KKIDEQIESKKQIIIDFINSAILRLEQQKIQYQASISELESGMFSDTSYKEIEFTKLN
                     RLYGINESFYYEMIRTKAEYMISQAGLVSNNIILEKASVPRTQKSPILTTIMLLSIL"
     gene            complement(1532..2308)
                     /locus_tag="PLK75_11040"
     CDS             complement(1532..2308)
                     /locus_tag="PLK75_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOY39822.1"
                     /translation="MQLRLISIFFTAAVLMSLSSCKILRPTEMFKENKDYPISEFKPS
                     EKEYRIKPFDILSIRITTNDGYSLIGVGGGTQAGTQSDYRMLQSGLEYLVEYDGLVKL
                     PTLGRTNMAGFTIREAEQELETMYSEYYQNPFVLIDVTNRKVFVFYDGGTRAHTITIP
                     KENITLVEAISRLGGLTDISRAYRIKLIRGDITNNPEVYYWNLSTLDELKETNLLLQA
                     NDVIYIDSKPQYVTKILKEIAPYLTLATTALSVYGIFFKK"
     gene            complement(2367..3551)
                     /locus_tag="PLK75_11045"
     CDS             complement(2367..3551)
                     /locus_tag="PLK75_11045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39823.1"
                     /translation="MTKVLRIINRFNLGGPTYNVAYLSKYMEPEFETLLVGGAIDESE
                     ASSDYIIKKLGLNPVVIPEMKREINGKLDLIAYRKIKKLIKEFKPDIVHTHASKAGFL
                     GRYAAINCNVPIIIHTFHGHIFHSYFNRYKTMIFKGLERNLARKTSAIIAISNLQLQE
                     LCYEHRIAPYDKFRVIPLGFDLERFQDSYSLKRESFRKEYNLDDDEVAVSIIGRLVPV
                     KNHPLFINALAKAKKNSAIKLRAFIVGDGNLIGDLQGLANQLGLSCTTIENKKQKADI
                     TFTSWIKDADYVFAGSDIVALTSYNEGTPVSLIEAQATNKPIVSTKVGGIEDVVISGT
                     TALLSENDNLDAFADNLTLLVNNKELREDMSRAGYEFVKNKFHYTRLVADMKDLYNQL
                     LK"
     gene            complement(3555..5438)
                     /gene="asnB"
                     /locus_tag="PLK75_11050"
     CDS             complement(3555..5438)
                     /gene="asnB"
                     /locus_tag="PLK75_11050"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOY39824.1"
                     /translation="MCGITGIYAFNKSGTDKFRFVDAAVKTLSKRGPDGYGTYQGNNV
                     RLGHTRLSIIDTSDAGSQPFTDPTGRYTLIYNGEFYNYKEFRHQYEKEGIHFRSTSDT
                     EVLLYLLIKEGKEGLKKLNGCFSIAFYDNDKEELLLARDRMGINPLLYYLDKNVLIFG
                     SEMKSILAYNIEKNIDSSSLYAYFQLNYIPGEKSIFQNVFRLTPGAYLEVSKNSVNKE
                     RFYQIPNSGKLDISLNEAESHLISLLDASVQRRLVADVPLGSFLSGGIDSSIIVALAS
                     RHTSHLNTFSIGYSDEPFFDETNYAELVAKKFNTNHTVFKLSNDDLLHQLPEVLDYLD
                     EPFADSSAIAVHVLSMHTRKTATVALSGDGADEMFAGYNKHLAHFKLIHPSAKEHLAL
                     YFSWLWSNLPQSRNTKYSNLFRQLDKFCQGAKLTAKERYWNWAGITQENQIHFLLKDT
                     FLNKTGGKARKEEMLQYFEGKSEMREILYTDMHMVLQNDMLTKVDLMSMANSLEVRVP
                     FLDHTVVDFAFQLPDAYKINLSHKKIILQEAFRSMLPAELYHRPKHGFEVPLLKWFRN
                     ELWSMIDNDLLSERFVTEQNVFKYEAIEKLKDKLTSNNPGDSQAQIWALLVFQNWWKK
                     YYV"
     gene            complement(5445..6833)
                     /locus_tag="PLK75_11055"
     CDS             complement(5445..6833)
                     /locus_tag="PLK75_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOY39825.1"
                     /translation="MRIALLIFFCLASLLTFSQNSLLPEDVSTIKTGFDNSKPADRAL
                     LNAVSGNSIIKLATVRSITGKEQHQFSDKVVVKGITDQKSSGRCWMFTGLNTLRPQII
                     EAHSLQNFEFSTNYLFFWDQLEKANMFLEGIIATAGLPLNDRKVEWLLKEPIGDGGVW
                     NSWANLIVKYGVVPKHVMPETYHSENTAQLNQFLKRKLREQALMLRDECNSGNSEEQL
                     HALKLEMLKEIYRILVLCLGEPPEKFEYGFPDKNGKVKEMVEYTPLSFFQKNFKEFDL
                     TNFVMLMNDPTREFYKLYEIESDKNVVEGISWRYINLPADEIKKFAILSIKDNTPMYA
                     SCDVGKFRNNDDGTLDLNNYDFESLLGVPFSMSKKERIESYDSGSSHAMLLIAVDINR
                     DGNPIKWQFENSWGASSGYNGYLSFTDAWFSEYLFRLVVNKKYVGNDVLEVLKQEPII
                     LPPWDPMFSQDE"
     gene            complement(6985..7389)
                     /gene="trxA"
                     /locus_tag="PLK75_11060"
     CDS             complement(6985..7389)
                     /gene="trxA"
                     /locus_tag="PLK75_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOY39826.1"
                     /translation="MKNLKLALILFVSLSLINCTGSAKQEDSDNVVVLQSESDFDKII
                     SGSTTVIVDFYADWCRPCKMQGPILQEIASEMNGKIIVLKVNVDKFPDIARRYQVTGI
                     PSLIMFNKGEHVWDAVGLQEKETIISAYKLNT"
     gene            complement(7386..7595)
                     /locus_tag="PLK75_11065"
     CDS             complement(7386..7595)
                     /locus_tag="PLK75_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOY39827.1"
                     /translation="MKSLIKKYYLLVLFTIIGATGGFLYWKFVGCASGACPIKSLWYL
                     STLYGSIFGFLIGSLLSTSIKKSQK"
     gene            complement(7634..8284)
                     /gene="elbB"
                     /locus_tag="PLK75_11070"
     CDS             complement(7634..8284)
                     /gene="elbB"
                     /locus_tag="PLK75_11070"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOY39828.1"
                     /translation="MKRIAVILSGCGQLDGSEIHEATLSLYALSKHGFDYHCFAPDID
                     QYHVINHFTREVVPEKRNVLIESARIARGNIKPLSDYNASNFDGLLLPGGYGAAKNLC
                     SYAVEGENFSVNSQVENALLQTYKAGKPIVALCIAPVIIAKVLKADVTIGTDAVTSGH
                     IMHVGGKNKPCNATGYYYDTDKNIITTPCYMEAKSIAEVGAGIENAVLKLKEITSV"
     gene            complement(8289..>8595)
                     /locus_tag="PLK75_11075"
     CDS             complement(8289..>8595)
                     /locus_tag="PLK75_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39829.1"
                     /translation="LTAGSWLLADAFSLSNSVAIEKTNALDNNIAIYPNPSKGTVYIT
                     NAKNSDIVVYNMIGKRVMSVNNFNSDRLNISSLPNGNYVFQVLKNNAVTTQKIILSK"
BASE COUNT         2436 a         1708 c         1607 g         2844 t
ORIGIN      
        1 atcagtatcg aaagaagcat aatggtggta agaatgggcg acttttgagt tctgggaacc
       61 gatgcttttt cgagaatgat attgttcgaa acaagtcctg cctgagatat catgtattca
      121 gccttagtgc ggatcatttc atagtaaaag ctttcgttga tgccatacaa tcggttgagt
      181 tttgtaaatt caatttcctt gtacgatgta tctgagaaca tgcccgattc aagttcgctg
      241 atcgaagcct gatattgaat cttctgttgt tcgagcctaa ggattgcact atttatgaaa
      301 tcaataatta tttgtttttt cgactcaatc tgctcgtcaa tttttttaat tctgaaatta
      361 ttcccggtga tatcgaacat cagttcttct ttctcttcaa tcaacgattg cagggtgctt
      421 aacagggatg ttaaaaatct ttcagagttt gagccagtaa ccaaggccat caactcataa
      481 atgttaagtt tcggattatt ggatatgtct ttttttactt tttcaagggt tgcaatttca
      541 agttcaatgt caatttgaag gtcttccagt tccgagactt tcgatgtgta catggtaact
      601 ttattggtgc catattgatc cagcccgggg gggattttgt tttcccgttt aaaatcactc
      661 aggtcttttt cggtttgatc aagctcttta taaatagttt ctaactgctg gtcgatgtat
      721 tttatcacat ttgttgcact ttcttttttc cggcttaaat cgtattcaag aaacttttct
      781 gcaatagtat tggtaatttc agcagcttta agtgcattgt tatcactata cataatcagt
      841 atagttctgg cggcatcatt ctgaaccctg acaataaggt tggcagatat tttttgcaaa
      901 aaagtgcccg gattgttaat tacaaaaaag tatttcgatt tagcgtctga ttcttgttca
      961 atcctgatag tttttggcga atcgtagtga atgaatattt cgccgccaaa aatagggtgc
     1021 cagtcttcag gtgacagaat atattcatag tcttcgtttc ctattttata acgcagctga
     1081 tatttatcct taataaaatt gatattgatt ctcgtgttgt aaataacagg gttattaatg
     1141 ttgacatata cggtgaaagg agactgctta tatatctcac ttgagataaa cgttcccttt
     1201 ttaaaataac taatatccag tggcaactcc gtgaagcatt cccttaaaaa cgtctttgat
     1261 tgaataagct ctgttaccga tgtaagatcg ctttgcgata cctcgttgtc aagctttaag
     1321 aactgattgg ttttgttttc ttccttgacc tgaatgattg accgtgtttc atatatcggt
     1381 agagtgtacc tgtaataaag aaagccagca agaactgatg caatcaggat gagaacaacc
     1441 aaccacaggc tcatgaagaa aattctgatg aatactctta catcgaagct ttggttaaat
     1501 acaggaattt ttgctctctt cattgctgta attacttttt aaagaaaata ccgtaaaccg
     1561 aaagtgctgt ggtggctaat gtaagataag gggcgatttc tttcaggatt ttggtaacat
     1621 attgtggttt gctatcaatg tatattacat catttgcctg aagaagaagg tttgtttcct
     1681 taagttcgtc taaagtggaa aggttccagt agtacacttc cgggttgtta gtgatatcgc
     1741 ctctgataag ttttattcta taggctctgc ttatatcagt cagtcctccc agtcgactta
     1801 tcgcttcaac caaggtaata ttttcctttg gaatggttat cgtatgagcc cgggtgccac
     1861 cgtcgtaaaa aacaaatact tttcggttgg taacatcaat taaaacaaat ggattctgat
     1921 aatattcaga atacatggtt tcgagttcct gttcagcttc cctgatcgtg aacccggcca
     1981 tattggttct acccagtgtc ggtagcttta ccagtccatc gtattcaaca aggtactcca
     2041 atccactttg aagcattctg taatcacttt gggttcctgc ttgcgttcct ccgcccactc
     2101 caataagtga atacccatcg ttggtggtaa ttctaatcga taaaatatca aaaggtttga
     2161 ttcgatactc tttttcagat ggcttgaatt ccgatatggg gtagtctttg ttttccttga
     2221 acatctctgt tggccgcaat attttgcagg aacttaacga catcaggact gctgcagtaa
     2281 agaatatgct gatcaatcgt aattgcattt tgcaatgatt tgaatatttt tcaaaattaa
     2341 aaaaataatt gtaaaataca accaagctat ttgagtaatt gattatacaa gtctttcata
     2401 tcagctacaa gccgagtgta atgaaatttg ttttttacaa actcatagcc agcacgcgac
     2461 atatcttccc taagttcctt gttgttgaca agtaaagtaa gattatcggc aaaagcatcc
     2521 agattgtcgt tttcgctgag caatgcagtt gtaccgctta taaccacatc ttcgatgcct
     2581 ccaacttttg tactgacaat aggtttgttg gttgcctggg cttcgatgag tgataccggt
     2641 gtcccttcat tgtaagaagt taaagcaaca atgtcggagc ctgcaaaaac atagtctgca
     2701 tctttgatcc acgaagtaaa agtaatatct gctttttgtt ttttgttttc aatggttgtg
     2761 caactaagtc ccaattgatt tgcaagacct tgtaaatcgc cgataagatt gccatccccc
     2821 acaataaaag cgcgaagttt tatagcagaa tttttctttg ctttggccag agcatttata
     2881 aacaatgggt ggttttttac aggaaccagg cgccctataa ttgaaacggc aacctcatca
     2941 tcatcgagat tatattcttt tctgaatgat tcccgcttaa gactgtagga atcctgaaat
     3001 ctttccaggt cgaatcccag aggaattact ctgaatttat cgtaaggggc aatgcggtgt
     3061 tcgtaacaaa gttcctggag ctgaagattg cttatcgcaa tgattgctga ggtttttctg
     3121 gctaaattcc gctcaaggcc tttgaaaatc attgtcttat agcggttaaa atacgaatga
     3181 aatatatgcc cgtgaaatgt gtgaattata ataggtacat tgcaattaat tgcagcatac
     3241 ctgcccagaa atccggcttt tgaagcatgg gtatgaacaa tgtctggttt aaattctttg
     3301 ataagttttt ttatctttcg ataagcaatt aaatcaagtt tcccgttgat ttctcgcttc
     3361 atttcaggga ttaccaccgg gttaagtcca agttttttta ttatataatc ggaacttgcc
     3421 tcactttcat caatggcacc tcccacaagc agggtctcaa attcaggctc catatatttg
     3481 ctgagatatg caacgttgta tgttggcccg ccaagattaa atctgttaat aatacgtaga
     3541 acttttgtca taatttaaac ataatatttt ttccaccagt tctggaatac aagcagagcc
     3601 caaatctgag cctgcgaatc acccggatta ttacttgtaa gcttatcttt aagtttttcg
     3661 attgcttcgt acttgaatac attttgctca gttacaaacc tctccgataa caagtcgttg
     3721 tcaatcatac tccaaagttc attgcggaac catttcagaa gcggaacctc aaaaccatgt
     3781 ttcggtcggt gataaagctc tgccggcagc atgcttctga aagcttcctg taaaattatt
     3841 tttttatgag ataagttaat tttataagca tcaggtagtt gaaatgcaaa gtcaacgaca
     3901 gtgtggtcga ggaagggtac cctaacctca aggctatttg ccatcgacat taaatcaact
     3961 ttggtaagca tatcgttttg cagtaccata tgcatatccg tatacaatat ctcacgcatt
     4021 tcgcttttac cttcaaaata ctgaagcatt tcttcttttc ttgctttacc tccagtttta
     4081 tttaggaaag tatccttcaa taaaaaatga atctgatttt cctgtgtaat gccggcccaa
     4141 ttccaatatc tttcctttgc tgtcaattta gctccctggc aaaatttgtc gagttgtcgg
     4201 aataagttgc tgtattttgt gtttctcgac tgaggcaggt tgctccaaag ccatgaaaag
     4261 tacaatgcaa gatgctcttt tgccgagggg tgtattagct taaaatgcgc caggtgtttg
     4321 ttgtagcctg caaacatttc gtcggcacca tcacccgata atgcaacagt tgctgttttg
     4381 cgcgtatgca tgcttaaaac atgcaccgca atggctgaac tgtcggcaaa gggttcgtca
     4441 agataatcga gtacttccgg taactgatgt agcaaatcat cgttcgaaag cttgaaaacc
     4501 gtatggtttg tgttgaattt tttagctaca agttctgcat aatttgtttc gtcaaagaat
     4561 ggttcatcgc tgtaacctat cgagaatgtg ttcaggtgcg atgtatgccg gcttgccaat
     4621 gctacaatta tcgatgaatc aatgcctccg ctgaggaacg acccgagagg aacgtctgca
     4681 actaatcggc gctgtaccga tgcatcgagc aaggaaatca gatgactttc tgcttcattt
     4741 aagctaatat caagcttccc tgaattggga atctggtaga atctttcttt attcacgctg
     4801 tttttactga cctcaagata tgccccggga gttaagcgaa acacattttg aaagatactt
     4861 ttttcccccg gaatataatt aagctggaag taggcatata acgatgatga gtcaatgttc
     4921 ttttctatat tatacgcaag aatcgatttc atttcagagc caaaaatcaa tacatttttg
     4981 tcgagataat acagtaacgg gttgattccc attctatcgc gtgcaagcag gagttcttct
     5041 ttatcgttgt cgtaaaatgc aatcgaaaag catccgttaa gttttttaag tccttctttt
     5101 ccttccttaa taaggagata aagcaggact tctgtgtcgg atgtacttcg gaaatgaatt
     5161 ccctcttttt cgtattggtg gcggaattct ttataattat aaaattcacc gttgtaaata
     5221 agggtatatc tcccagtcgg gtcggtaaaa ggttgagaac ctgcatcgga tgtgtcgatt
     5281 attgaaagcc gcgtatgacc aagacggaca ttattaccct gatatgtccc atatccgtca
     5341 ggaccccttt tgctaagggt tttaacagca gcatcaacaa acctgaattt atcggttcca
     5401 cttttattaa atgcatatat acctgttatt ccgcacatat ttttttattc gtcttgagaa
     5461 aacatcggat cccatggtgg aagaataatg ggttcttgct taagcacttc aagcacatca
     5521 ttaccgacat attttttatt gacaaccagc ctgaaaagat actccgaaaa ccatgcatcc
     5581 gtaaagctaa ggtatccatt atagccagaa gatgctcccc agctgttttc gaactgccat
     5641 tttatcggat tgccatccct attaatatca acagctataa gaagcattgc gtggctcgat
     5701 ccactatcat agctttcaat tctttccttt ttgctcatac taaacggtac tccaagcaaa
     5761 gattcaaaat cataattatt aagatcaagg gtgccgtcat cgttattcct gaatttaccc
     5821 acatcacatg aagcatacat gggtgtatta tcttttatac ttaatatggc aaacttcttt
     5881 atttcgtcag caggaagatt tatatatctc cacgaaatac cttctaccac atttttgtct
     5941 gattcaattt cgtatagctt gtaaaactca cgtgtagggt cattcatgag catcacaaaa
     6001 ttggttaaat caaattcttt aaaatttttc tggaaaaacg acaacggagt atattcaacc
     6061 atttctttaa ctttcccgtt tttatcgggg aacccgtatt caaatttttc aggaggctct
     6121 cccagacata gcactaagat gcgatatatt tctttaagca tttccagttt gagtgcatgc
     6181 agctgttctt cactattccc agaattgcat tcatcacgca gcatcaatgc ctgctcacgc
     6241 agcttacgtt ttaaaaactg atttaactgg gctgtgtttt ctgaatggta ggtttcaggc
     6301 ataacgtgtt ttggtaccac tccatattta acaatcaggt tagcccatga attccaaact
     6361 cctccatcgc caataggttc ttttaatagc cactcaacct tgcggtcgtt cagggggagt
     6421 cctgctgttg caattatgcc ttccagaaac atatttgctt tttcgagttg gtcccaaaag
     6481 aaaagatagt tggtggaaaa ctcgaaattc tgaagggaat gggcttcaat tatttgcggg
     6541 cgcaatgtgt tcaaacctgt aaacatccaa caccttccgc tgcttttctg gtcggtaatc
     6601 cctttaacaa ccactttatc cgaaaactga tgctgctctt taccggtaat acttcgtaca
     6661 gtagcgagct taataatact gttgccgctt accgcattca gcaacgccct gtcggcaggt
     6721 ttcgaattat cgaatcctgt ttttatagtg cttacatctt ccggtaataa ggagttttga
     6781 gaaaaagtca acaacgatgc taagcaaaaa aatatcaaca aagctattct catcccaact
     6841 caacagttaa ttaactcgta aagttataaa aatactgaat agttgaaata taatttggtt
     6901 aaaaagttat atgttgctat agatataaca ttaataaatt cgcattatta atgcaaggtt
     6961 ttaaatgtat atcaccttcg gtttttaggt attcaattta taggcgctga ttatcgtttc
     7021 cttttcctgc agtccaacgg catcccatac gtgttctccc ttattaaaca taataagcga
     7081 tggaatacct gtaacctgat aacgccgtgc aatgtccgga aacttatcta cattaacttt
     7141 aagcacaata attttcccgt tcatttcaga agcaatttcc tgcaaaatcg ggccttgcat
     7201 tttacagggt ctgcaccagt cagcatagaa gtcaacgatt actgttgttg aacctgaaat
     7261 tattttgtcg aaatctgatt cactttgcag cacgaccaca ttatcggagt cttcctgctt
     7321 tgctgacccc gtacaattta ttaatgacag agaaacaaat aaaataagtg ccagttttaa
     7381 atttttcatt tttgagattt ttttatagaa gtggaaagga ggcttccgat aagaaagcca
     7441 aatattgagc cgtataaagt actcaaatac cacaacgatt taatagggca tgcgccggaa
     7501 gcgcaaccaa caaatttcca gtacagaaat ccgccggtgg ctccaataat ggtaaacaat
     7561 accaagagat agtacttttt aattaagctc ttcattttta attttatgta taaccgcttt
     7621 ttttttacaa tgttcaaacc gaagttattt ctttcagctt cagtacagcg ttttctatac
     7681 ctgccccaac ttcggcaatg ctttttgcct ccatatagca gggagtagtg attatgtttt
     7741 tatcagtatc gtagtaatac ccggtggcat tgcagggttt gttttttcct ccaacatgca
     7801 tgatatgccc ggatgtaacc gcatcggtgc caatggtaac atcagccttt agaacttttg
     7861 caataattac aggagcaatg catagtgcaa caataggttt tccggccttg tatgtttgta
     7921 atagagcatt ttctacttgg ctgttcaccg aaaaattctc tccttctacc gcatatgaac
     7981 aaaggttttt tgctgcccca taccccccgg gtaagagaag cccgtcgaag ttcgatgcat
     8041 tgtaatcgct aagtggttta atattgcctc tggcgattct ggctgattca ataagaacat
     8101 tccttttctc cggaacaact tcgcgtgtga aatgattaat aacatggtac tgatcgatgt
     8161 caggggcaaa gcaatggtaa tcaaacccat gcttcgacaa tgcatacaaa cttagtgtag
     8221 cctcatgaat ttcgctgccg tcaagttgtc cgcaacccga tagaataact gcaattcttt
     8281 tcattatttt attttgatag aatgattttt tgagtagtca cggcgttgtt tttaagaacc
     8341 tggaatacat agttcccgtt gggtaaactc gaaatattca atcgatctga attgaagtta
     8401 ttcacactca ttacccgttt ccctatcatg ttataaacta ctatgtccga gttttttgcg
     8461 ttggttatat aaactgtacc cttcgatgga ttgggatata tggcaatgtt gttatccaaa
     8521 gcgttagttt tttcaatggc aacggaattc gaaagagaaa aggcgtctgc cagtagccaa
     8581 ctgcctgctg ttaat
//
LOCUS       DAOOQP010000126         8522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_10276_length_8522_cov_4.683477, whole genome shotgun sequence.
ACCESSION   DAOOQP010000126 DAOOQP010000000
VERSION     DAOOQP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10276_length_8522_cov_4.683477"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLK75_11080"
     CDS             <1..212
                     /locus_tag="PLK75_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39830.1"
                     /translation="FTTLMLGFPIGCNFRLWNHLTIKPGVSVLFPLTNRTIQIGSDIV
                     QQGPWGLEIGLRIGYQFRWKQNKVM"
     gene            complement(328..1227)
                     /locus_tag="PLK75_11085"
     CDS             complement(328..1227)
                     /locus_tag="PLK75_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOY39831.1"
                     /translation="MNWDIYIKQFHDYLMLEKSLSGNSVEAYISDITKLKQFAGMLNP
                     PRNADEISAGDLKSLMEYLGSLGIAARSQARIVSGIKSFYRFLELDGAVSTDPSLLLG
                     TPKLGLMLPVVLSIDEIDAIMHCIDLSQPLGHRNKAIIETLYSCGLRVSELTDLKISN
                     LYFNEDFIRVTGKGSKERLIPISTRAKSEIKYYFQSFRNHILPAKGHEDFVFLNRRGK
                     KLTRVMIFTIIKDLVTKAGIEKTVSPHTFRHSFATHLIEGGADLRAVQEMLGHESILT
                     TEIYTHIDREYLREAIIQFHPRS"
     gene            1267..1689
                     /locus_tag="PLK75_11090"
     CDS             1267..1689
                     /locus_tag="PLK75_11090"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOY39832.1"
                     /translation="MKKLLIINGPNLNLLGIRETELYGEKSLADIITDLRQTFIGFTI
                     DEIQSNSETEIVDVLQHASNNYHGVAINAGAFTHTSVSIRDAVAAINIPVVELHITNT
                     SARELFRATSLISPVCKGTINGFGYFSYHLAVYSLINL"
     gene            1695..2219
                     /locus_tag="PLK75_11095"
     CDS             1695..2219
                     /locus_tag="PLK75_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39833.1"
                     /translation="MDQQRTITKLRMLKISIMFSLILLVYAVVLGALLEMNKEMIMRS
                     LRDKATEALGSKYDNNMSVALKTCNDAFQYFVKSQENAVVFGVTGLVMALIFIRLPLT
                     QRQKSLPSLCFSFGSILYVFYYLGVGIILPSAAKLEVAKAYVSWLFYPAIILFSLGLI
                     LFIYHSFRKIVVES"
     gene            2298..2573
                     /gene="rpsO"
                     /locus_tag="PLK75_11100"
     CDS             2298..2573
                     /gene="rpsO"
                     /locus_tag="PLK75_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOY39834.1"
                     /translation="MSNYLDVEKKKEIFEKYGKSNNDTGSPESQIALFSHRINHLTEH
                     LKLNKKDFGTQRALMKLVGKRRRLLDYLHKNDIERYRAIVSELKLRR"
     gene            2644..4803
                     /gene="pnp"
                     /locus_tag="PLK75_11105"
     CDS             2644..4803
                     /gene="pnp"
                     /locus_tag="PLK75_11105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOY39835.1"
                     /translation="MYNEIKKVITLEDGREIEISTGKLAKQAHGSVVVKMGKTMLLAT
                     VVAADEAKPDVDFMPLSVEYREKYTAVGRFPGGFLKREGRTSDYEILISRLVDRALRP
                     LFPDDFHAETFVNIYLISAEKDATPEALAGLAASSALMVSDIPFHGPISEVRVARIDG
                     KFVINPSASLLAKADIDMIVAATYDNIIMVEGELDEASENDMIEAIRTAHDAIKVQCL
                     AQKELEAAVAKQVKRNYCHEVNDLELKQKVYDETFEKTYSVAKQLITNKTLRIQAFEA
                     IKEEFLSKYTEEELLTVKPLVNRYYHDIHKKAVRKMILDDQIRLDGRKMDEIRPIWGE
                     VDYLPTTHGSAIFTRGETQSLTAITLGNKLDEQVIDGAMIAGYEKFILHYNFPPFSTG
                     EARPIRGVGRREIGHGNLAHRALKRMVPEKGPDFPYTLRIVSDILESNGSSSMATVCA
                     GTLALMDAGIQIRKPVSGIAMGLITDSASGKYAVLSDILGDEDHLGDMDFKVTGTADG
                     ITATQMDIKVDGLSYEILAKALEQARKGRLHILGKILEIIPEPRKDYKPHAPRINTII
                     INKDQIGAVIGPGGKIIQGIQADTNTVISIEEVDNKGVVEICADNAENMNAALARIRA
                     IVALPEVNEIYNGKIKSITSYGAFVEILPGHEGLLHISEIEWRRLRSVDEVLKEGQMV
                     QVKLLEIDEKTGKMKLSRKVLLPRPPKPEEETKEAGQ"
     gene            complement(4839..5765)
                     /gene="miaA"
                     /locus_tag="PLK75_11110"
     CDS             complement(4839..5765)
                     /gene="miaA"
                     /locus_tag="PLK75_11110"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOY39836.1"
                     /translation="MHKNKLVCLLGPTAGGKTKLAAWFCERVGGEIISADSRQVYRGM
                     DIGTGKDISDYYVEGMLIPYHLIDIRNPGEKYNIFEFANDFKRVYKCISDVGKLPVLC
                     GGSGLYIESVIGNYQLLAVPTNDELRKKLQGKTLQELGIILSTYKKLHNHTDTDTIKR
                     AIRAIEIADYEKLHGKVMQSEPEFNNLIFGIAYDRETRRERITARLKERLGQGLLEEG
                     RQLLQNGLSLEDMEYYGLEYKYMALHLSGRLSLDEMAEKLNTAIHQFAKRQMTWFRRM
                     ERNGVKIHWIDPNMNFDKKLTYMLTKYQDTFC"
     gene            5844..6404
                     /locus_tag="PLK75_11115"
     CDS             5844..6404
                     /locus_tag="PLK75_11115"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOY39837.1"
                     /translation="MSHYFHHIRLLFSNPPKAWHNIKKDDGSYKRLFFGPVILVIAGM
                     SLCLLVGESLSMASTGLFIDIIKHALIFFVANLLSFYIISVFINAIISSFTGKKDMAR
                     VGQLLLYTLSPYYFVMSVFYIFPSLIFILLVPVYCFVLLWFGVKQLIEIPSDKITGFY
                     IVTALIVVGINLISHFVFNYAFLKVI"
     gene            6406..6849
                     /gene="smpB"
                     /locus_tag="PLK75_11120"
     CDS             6406..6849
                     /gene="smpB"
                     /locus_tag="PLK75_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOY39838.1"
                     /translation="MKAEPRTFKNKKAYFQYELKDFVTAGIQLKGTEIKSIRDGKVNF
                     TDSFCLVVDGEMWLKGLHIAEYVLGTCNNHNPVRDRKLLLKKREIRQFDRKVREKGYS
                     IIPVSLFIDENGRVKVEIALARGKKQFDKRETIKRKDIQRDLERI"
     gene            6965..>8522
                     /locus_tag="PLK75_11125"
     CDS             6965..>8522
                     /locus_tag="PLK75_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39839.1"
                     /translation="MRKAQTILLTAIMLASVSFSHSQNLVMNPSFESVNTGSLLCSWY
                     VSSGEFNAAVNNWTMPTGGSTDLFHSSLATSCFCSPWSTNASAVGQQTPRTGSAMSAI
                     FVYGAGGCTPYREYLQGRLSSPLVPGQQYTVEFYVSMADYSMYACNNIGVYFTTASFY
                     SSSMCVYSVTPQVNYTGIITDKTNWTLISMTFTATAAFQYFTIGNFYYDTGTTTTNVG
                     GSKAQTRYFVDDVSIMVSNVNPVITVNNPTICAGQSATLTATSSIAGTTFAWSNGATG
                     ATISVNPTSTTTYTVTGTAPSGTTGTATATVTVNPLPVVTASSNSPVCETQSLNFTGG
                     GGAAGATYTWTGPDGWTSNLQNPVRTPASVSMSGTYTLVVTNTTGCSSSNTTTVVVQA
                     RPNASINSVGPFCQSDSPVTLTAATGGGTWSGTGITGSTAGTFSPAVAGSGDHTITYS
                     VTTGACTGTSSITIHVDGDVNADISPAGPFCINASAVNLTAVSNGGTWSGNGITNAAT
                     GQFSPSVAGAG"
BASE COUNT         2479 a         1767 c         1802 g         2474 t
ORIGIN      
        1 attttaccac attgatgctt ggttttccga taggatgtaa tttcagacta tggaatcacc
       61 ttaccattaa acccggagtt tcggtgttgt ttccgctaac aaaccgtacc attcaaattg
      121 gtagcgacat tgtacagcaa gggccatggg ggctggaaat cgggctgaga atcggatatc
      181 agtttcgatg gaaacagaat aaagtaatgt aaccaggcca gtttataggc agttttggag
      241 tgaccaaact tcgtgctaac aatcgctttt ataaaccttg ccattgaaaa ttattcccaa
      301 atttgttctg attacagtat ttattgctta tgacctagga tgaaactgaa taatagcctc
      361 gcgcagatac tctctgtcga tatgcgtata tatttcggtt gttagaatcg attcatgacc
      421 caacatttcc tgaactgccc tcaaatcagc tccgccttca ataaggtgag tcgcaaatga
      481 atggcgaaaa gtatggggac ttaccgtttt ttcgatacct gcttttgtaa cgagatcttt
      541 aattattgtg aaaatcatta cacgtgtcag ctttttaccc cttcggttca aaaacacgaa
      601 atcttcgtgc cctttggcag gtaaaatgtg gtttcggaac gactggaaat aatactttat
      661 ctctgatttg gccctagtgc tgatcggaat aagcctttct ttacttccct tgccggtaac
      721 ccgaatgaaa tcctcattaa aatacaagtt tgatattttc agatcggtaa gctcacttac
      781 acgaaggccg caactgtaga gtgtttctat tattgcttta ttcctatggc caagaggttg
      841 gctaaggtcg atacaatgca taattgcatc gatttcatca atgctgagta ctaccggcaa
      901 cataagccca agtttagggg taccaagcag caaagatggg tcggttgata cagcaccatc
      961 caactcaagg aagcggtaga aggattttat tcctgaaact atacgtgcct gactacgtgc
     1021 tgcaatgcca agggaaccca aatattccat taacgacttt aaatcacccg ccgatatctc
     1081 atcggcatta cggggagggt taagcattcc tgcaaattgc tttaatttgg tgatatcgct
     1141 aatatacgct tctactgaat tacccgacaa cgacttctcc aacataagat agtcgtgaaa
     1201 ctgttttatg taaatatccc agttcatttc aacgccaaat taataaatta aattatattt
     1261 gtccacatga aaaaacttct gataataaac ggtccaaacc ttaatttatt gggaataaga
     1321 gagaccgaac tgtatggcga gaaaagcctt gcagatatta tcacagactt gcgtcaaact
     1381 tttatcggtt ttacaatcga tgaaattcaa tcaaattcgg aaactgaaat agttgatgtc
     1441 ttgcaacatg ccagcaataa ttaccacgga gttgccatca atgcgggtgc cttcacacat
     1501 acatcggtaa gcattcgtga cgctgttgct gcaattaata taccggttgt ggagttgcat
     1561 attactaata cctctgcgcg ggagctgttt agggcaacat cgctgatttc acccgtttgt
     1621 aaaggtacaa tcaatggatt tggatatttt tcatatcact tggcagtata ttcattaata
     1681 aatttataga ctttatggac caacagcgaa ctatcaccaa actcaggatg ctgaaaataa
     1741 gcatcatgtt ttcgttaatc cttttagttt atgcagttgt tttgggcgct ttactcgaaa
     1801 tgaataagga aatgattatg cgcagtctgc gcgataaagc aactgaagcg cttggaagca
     1861 agtacgacaa caatatgtcg gtggctttga aaacatgtaa cgatgcattt cagtactttg
     1921 taaaatcgca ggaaaatgct gtcgttttcg gagttacagg tctggtgatg gcattgatat
     1981 ttatccgcct cccacttacc caacggcaga aatcactccc aagcctctgt ttttcatttg
     2041 gatctatttt gtacgtgttt tattacctcg gagtgggtat tattcttccc tctgcagcaa
     2101 aacttgaggt cgctaaagcc tatgtgagct ggttgtttta ccctgcaatt attcttttta
     2161 gcctgggact aatattgttc atctaccata gtttccgaaa gattgttgta gaaagctgat
     2221 tattaaattg ctgtattaaa aaaaaaatag tatttttgca tccttattaa atgaattgat
     2281 tattaattgt ttaagaaatg agtaattatt tagacgtaga aaagaagaaa gaaatcttcg
     2341 aaaagtacgg aaagtctaat aatgacacgg gttcgcctga gagccagatt gcattatttt
     2401 cccaccgtat caatcacctt accgaacacc tgaaattgaa caaaaaagat ttcggaacgc
     2461 agagggcatt gatgaaattg gtagggaaaa gaaggcgtct gctcgattat ttgcataaaa
     2521 acgatatcga aagatataga gccattgtgt cggaattaaa actccgtcgg tagaagaaac
     2581 taaagaaagg caaggcactt gcctttcttt tttattaagg aacattaacc taaagatgaa
     2641 tatatgtaca atgagattaa aaaagtgatc acgttagaag atgggcgtga gattgaaatt
     2701 tccaccggaa aactggccaa gcaggcgcat ggttcagttg tggtgaagat gggtaaaacc
     2761 atgttgctgg ctacagttgt agcagctgat gaagctaaac cggatgtcga ttttatgccg
     2821 ctttcggttg aatatcgcga aaaatatacc gcagtggggc gtttccccgg tggttttctt
     2881 aaaagagaag gaaggacttc cgactatgaa atccttattt cgcgtctggt cgatagagcc
     2941 ttgaggccat tgtttccaga cgattttcat gctgaaacat tcgttaatat ttacctgatt
     3001 tccgccgaaa aagatgcaac cccggaagca ttagccggac ttgctgcctc atcggcatta
     3061 atggtgtcgg atatcccgtt tcacggacca atttccgaag ttcgtgttgc acgcattgat
     3121 ggaaagtttg tgattaaccc cagtgcaagt ttgctcgcaa aagccgatat cgacatgatt
     3181 gttgcggcta cttacgataa tataatcatg gtggaaggag aacttgacga agcatctgaa
     3241 aacgatatga tagaagctat cagaaccgcc cacgatgcca taaaggttca gtgccttgct
     3301 caaaaggaac tcgaagctgc tgttgccaaa caagtgaaga gaaattattg ccacgaagta
     3361 aatgaccttg aactaaagca aaaagtatac gacgaaactt ttgaaaaaac ctatagtgta
     3421 gctaaacagt tgattacaaa taaaaccttg cgtattcaag ctttcgaagc aataaaggaa
     3481 gaattcctgt cgaaatatac cgaagaagaa ttgcttacag tgaaaccatt agtaaatcgt
     3541 tattatcacg atattcacaa aaaagccgtt cgcaaaatga ttcttgatga tcagattcgt
     3601 ctcgacggcc gcaaaatgga tgaaatccgt ccaatttggg gcgaagttga ctatttaccc
     3661 accacgcacg gatcagccat ttttacccgt ggcgaaacgc agtcgcttac tgctattacc
     3721 cttggaaata aattggatga gcaggtaatt gacggtgcga tgattgcagg atacgagaag
     3781 tttatattgc attataattt tccacccttt agcactggtg aagcacgccc catacgtggt
     3841 gtaggccgtc gtgaaattgg tcatggcaat ttagctcaca gagcattaaa aagaatggtt
     3901 cctgaaaaag gcccagactt cccctatacg cttcgtattg tgtcggacat tctggaatcg
     3961 aatggttcat cctcgatggc aacagtatgc gccggaacac tggctcttat ggatgccggt
     4021 attcaaatcc gtaaacccgt ttctgggatt gctatgggcc tgattactga ttccgcttca
     4081 gggaaatatg ctgtacttag cgatattctg ggcgatgaag atcatcttgg cgatatggac
     4141 tttaaagtta caggaacagc cgatggaata accgccacac aaatggacat taaagttgac
     4201 ggactttcct atgaaatcct tgccaaagcg ttggaacagg ctcgcaaagg ccgcttacat
     4261 attctcggga aaattcttga gataattcct gaaccgcgga aagactataa gccgcatgca
     4321 cccagaatta atacgattat tattaataag gaccagattg gagcagttat tggccccggt
     4381 ggtaaaatta ttcagggaat tcaggctgat accaatacag taatcagtat tgaggaagtc
     4441 gataacaaag gtgttgttga aatttgcgct gataatgccg aaaatatgaa tgctgcactg
     4501 gcaagaattc gagccatagt tgctcttccc gaagtaaacg agatttacaa tggtaaaatc
     4561 aaatcgatta cttcgtatgg tgcctttgtt gaaatattgc ccgggcatga aggactctta
     4621 catatttccg agattgaatg gcgcagactt cgttcagtag atgaagtgct gaaagaaggt
     4681 cagatggttc aggtgaaact cctcgaaatt gatgagaaaa ccggaaaaat gaaattaagc
     4741 cggaaagtac tgttaccaag gcctccaaaa cccgaagagg aaacaaaaga agctggtcag
     4801 taggctccct tttctgatat ttcaatcaag acatcttcct aacagaaggt gtcttgatat
     4861 tttgtaagca tataagtgag ctttttatca aaattcatat tggggtcaat ccagtgaatt
     4921 ttaaccccgt tcctctccat tcgtcgaaac catgtcatct gtctcttagc aaattgatga
     4981 atggctgtat tcaacttttc tgccatctcg tcgagcgata accggccaga aagatgcagt
     5041 gccatatact tatattctaa tccgtagtat tccatgtctt cgaggctcag accgttttgc
     5101 agcaactgtc tcccttcttc cagcaatcct tgtccgagcc gctcctttag tcgggcagta
     5161 attcgttccc tgcgtgtttc tcggtcgtag gcaatcccaa aaatgaggtt gttgaattcc
     5221 ggttcagatt gcattacttt accgtgtagc ttttcatagt ctgctatttc tattgccctg
     5281 atagcacgct ttatcgtatc ggtatcggta tgattatgca gctttttgta ggtcgaaagt
     5341 attattccca actcctgaag ggtttttccc tgtagttttt ttcgcaactc atcgtttgtt
     5401 ggaactgcca ataattgata gtttccaata acggactcga tgtataaacc acttcctcca
     5461 cataagaccg gtagcttgcc aacatcggaa atacatttat aaacacgctt aaaatcattt
     5521 gcaaattcaa aaatgttgta cttttcaccc gggtttcgaa tgtcaattaa atgataagga
     5581 atcagcatcc cctcaacgta ataatctgag atgtctttac cggtaccaat atccattcca
     5641 cggtaaactt gtcggctatc tgcactgata atttctccac caacccgctc acaaaaccat
     5701 gctgcaagct tcgtttttcc tccggcagtg ggtccaagca gacaaaccag tttattctta
     5761 tgcattgttc acaatttaca caagtaaatt tatgaaaatt agattaattt tgtgggctga
     5821 atcagaaata ttatttattc accgtgagcc actactttca ccatatcaga ctgctttttt
     5881 ccaacccgcc gaaagcttgg cataacatta aaaaagatga cggcagttac aaaaggcttt
     5941 ttttcggacc tgtgattctg gtaattgctg gaatgagcct ttgcttactg gtaggagagt
     6001 cgctgtcgat ggcctcaacc gggttgttta ttgatattat taagcatgct ctgatattct
     6061 ttgtggcaaa tttactcagc ttttatatta tttctgtatt tattaatgcc attatttcca
     6121 gctttacagg taaaaaagac atggccaggg ttggtcagct gctgctttat accttgtcgc
     6181 catattactt tgtgatgtcg gtgttttata ttttcccgag cctgattttt atcctcttag
     6241 tacctgtata ctgcttcgta ttgttatggt tcggagtaaa acaactcatc gaaatccctt
     6301 ctgataaaat tactggtttc tatattgtaa ctgccttgat tgttgtggga atcaacctta
     6361 tttcacactt tgtttttaac tatgcattcc ttaaagtgat atgacgtgaa agctgaaccc
     6421 cgaacattca aaaataaaaa ggcatacttc cagtacgaat tgaaggactt tgttaccgct
     6481 ggcattcagc tgaaaggcac cgaaataaaa tcgattcgag atggcaaagt gaatttcact
     6541 gattcgtttt gtctcgttgt tgatggcgaa atgtggttaa aaggtttaca catagccgaa
     6601 tacgttttgg gcacctgcaa taatcacaac ccggtgagag accgtaaact cctgctcaag
     6661 aaaagagaaa tccgacagtt cgaccgtaaa gtcagagaaa aaggttactc catcattccg
     6721 gtatcactgt ttatcgatga aaacggacgc gttaaggttg aaattgccct tgcccgcggt
     6781 aaaaagcagt tcgacaaacg cgaaaccatt aaacgaaaag acattcagcg cgatttggaa
     6841 aggatttgat acctattctt attccattgc aaatgcaaac aatatgcgaa ttctcatcat
     6901 cggtaaatta attttcattt ataaactttt attcttaatt tagtaagtct ataaatttat
     6961 aataatgagg aaagcccaaa ctatattgct gactgccata atgttggcca gtgtcagttt
     7021 ctcacattct cagaaccttg ttatgaaccc cagctttgag agcgtaaaca caggcagctt
     7081 gctctgcagt tggtatgttt cgtccggaga atttaatgct gccgttaata attggaccat
     7141 gccaaccggt gggtcaaccg atctttttca ctcttcgttg gcgacgagct gtttttgcag
     7201 tccctggtca acaaatgcaa gtgctgttgg gcagcaaaca ccacgtacag gcagtgcaat
     7261 gagtgcaatt tttgtgtatg gggcaggagg gtgtacgcct tatcgcgaat accttcaggg
     7321 caggctgtca tcacctttgg ttccggggca gcaatatacc gtggagtttt atgtttcaat
     7381 ggctgattac agtatgtatg cctgtaataa catcggagta tatttcacta ccgcctcatt
     7441 ttacagcagc agtatgtgtg tatatagcgt caccccgcag gtgaactata ctgggattat
     7501 cactgataaa accaattgga cgctgatttc gatgactttt accgcgactg cggcattcca
     7561 atattttacc atcgggaatt tttattacga cacaggaact actactacca atgtaggtgg
     7621 ctctaaagca cagacgcgat attttgtgga tgatgtttcg ataatggttt caaacgttaa
     7681 cccggtgatt acggtaaata atccaaccat ctgtgcaggg caaagcgcga ccttaacggc
     7741 aaccagctcg attgccggga ctacttttgc ctggagtaac ggtgcaaccg gagcaaccat
     7801 cagtgttaac ccgacctcaa ctactaccta tacggttacg gggacagccc catcaggcac
     7861 aacaggtaca gctactgcaa cggtgactgt gaacccatta ccagtggtta ctgcatcaag
     7921 caacagccct gtgtgtgaaa cacagtcact aaattttaca ggcggtggcg gagctgcagg
     7981 agcaacctac acatggacag gacccgatgg ttggaccagt aatttgcaaa accctgtcag
     8041 aacaccagct tctgtttcca tgtcggggac atatacactg gtggtaacca atactacagg
     8101 atgcagcagc tcaaatacaa ccactgttgt tgtacaggcc cgtccgaatg caagcataaa
     8161 ttctgtcgga ccattctgcc agagcgattc acctgttact cttaccgctg ctacaggagg
     8221 tggtacatgg tcgggcactg ggattacagg cagcacggca ggtactttca gtcccgccgt
     8281 tgccggttcg ggcgatcata cgataaccta ttctgttaca accggagcat gcacaggtac
     8341 gagcagtatc acaattcatg ttgatggaga tgtaaacgca gatattagcc cagcaggccc
     8401 attttgtatt aatgcttctg ctgtgaatct gactgccgtt tcgaatggag gcacctggtc
     8461 gggaaatggt attaccaatg cagctacagg gcagttctca ccctcggtag ctggtgcggg
     8521 ta
//
LOCUS       DAOOQP010000127         8186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_10815_length_8186_cov_5.047227, whole genome shotgun sequence.
ACCESSION   DAOOQP010000127 DAOOQP010000000
VERSION     DAOOQP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10815_length_8186_cov_5.047227"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /gene="vorB"
                     /locus_tag="PLK75_11130"
     CDS             <1..856
                     /gene="vorB"
                     /locus_tag="PLK75_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOY39840.1"
                     /translation="MTSSSSPGISLKQEGISYIAGAELPCLIVNVVRGGPGLGTIQPS
                     QSDYFQAVKGGGHGDYKLIVLAPASVQEMYDFVSLGFEVAMKYRNPVMILSDGVIGQM
                     MEKVELGPQKNRRSREEVIKSAPWATTGKPASRPHNIITSLDLIAANQEKFNKKLIEK
                     YKTIEQNEVRFEELYMDDAKYAFVAYGSSSRICQSAIKIARKQGIKVGLIRPITLFPF
                     PKKQIVKRAEKVKGFLSVEMSAGQMIEDVKLALNGIRPAEHFGRNGGIVPTPEEVVEA
                     LKHNFIGG"
     gene            869..1648
                     /locus_tag="PLK75_11135"
     CDS             869..1648
                     /locus_tag="PLK75_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY39841.1"
                     /translation="MTLKDIIKEENLVYSRSKVMTPNELSFCPGCGHGTAHRLIAETI
                     EEMGIQSEVIGVSPVGCSVLMYNFMDVDMQQAAHGRAPSLATAIKRLMPDKYVFTYQG
                     DGDLAAIGTAETIHACNRGENITIVFVNNGIYGMTGGQMAPTTLPGMRSSTSPYGRDV
                     SMMGNPLKMTELVAQLAGTYYVTRQSVHTPRNVRRAKKAIRIAFENQKLNKGLSFVEI
                     VSNCNSGWKLPPVKANEWMEENMFPFYELGDIKVDGKLVTK"
     gene            1661..2212
                     /locus_tag="PLK75_11140"
     CDS             1661..2212
                     /locus_tag="PLK75_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOY39842.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGLMQGQEVSWMPSYGPEMR
                     GGTSNVTVIISDERINSPILTQFDTAIVLNQPSMDKFENMVKPGGLLLYDPNGITSPP
                     TRKDINIYKVEATDTAVKMGIAKVFNMIVLGSFIKIKPIVKIENVMKGLKKSLPPRNH
                     NLLPMNEDAIKKGLEIVEAVNVL"
     gene            complement(2290..7419)
                     /locus_tag="PLK75_11145"
     CDS             complement(2290..7419)
                     /locus_tag="PLK75_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOY39843.1"
                     /translation="MKNPIVCIVFLILSISVFSQQRSIDIELLEACKNNNYEEAKRLI
                     DDGADVNVKDENGYTSLHFATANEDGLEIAKLLIEKGADVNALTCKMKEPRYRRVRLL
                     GGDERKKESYSPLHFAVENNNGYEIVKLLIENGANVNAKISKNVRESAGFTPLHIAIK
                     CIGNSKIVKLLIDNGADVNATTKTGETSLHIAVEKSDFRITQLLIDNGAKINVKNKYN
                     VTPLCISMYNDEFEMVDLLLENGADINTTCDMEYGEGITPLHYAATKNGSYKMAKFLI
                     ENEANINIRTKSPKRIDTQMEFLGWTPLHIAAANNNAEFVSLLLKNGADTSLWNGEGL
                     SALEISRNFSSIDVLQLLANTDHEIFLNYWAKDFTSLEHLITQKPLIVDTKDSNGQTI
                     FHNAIKDNQTEVLKLLYSNKSLVNTTNSEEQTLLVYALLLGNTDVAAKLIDMGADMNK
                     PDAKGYTPYTIAKLRNQTNIVTALISQNADTLSSIKPVLTTMIGHNNTVKSVCLSTDG
                     KLLASCSDDNTIKIWDYPSGVLIKTFAGQHQEISCISFSEDGRLLVSADETGEMNLWD
                     VCSGVLLRTFGENNGSFESIAFSPDGRYIATIDDNAYCKLWDVQNGNLLWTYSEDYYS
                     FRFSPCGTKIVFDSDNDVKLYAVQTGALLLNLSGHTNFVNSVCFSPDGKLLASGSDDS
                     TIKLWDVQNGMLINTIQGNTEHFFYVCFSPDGKKLASKSSCGTLKIWDLQNDSLIHNF
                     NLKENYGSLCFSQNGEFIAEISANSFALWDLISGKEVDSKSSNGFEGFISPDFMSYVQ
                     IADNNIEIKDIENNIPLNNNKSNGFFVNSVSYFKQFDMLAMHTASDELKLLNVTNGNF
                     INHLDGVSSDNFGWNDNVSPDGTLIAKVNNSNTIELIEVQTGIMSKEFKGHDNYVRRI
                     LFSYDGKYLASCGSQSYKIFNVESGNSVKEFQLDDQHWKQGTSNDICFSPNENLLAMA
                     YSDRSILNWDLPTESLSAMYAYSDFGILVWDVQSEQIRCILNGHNDYVSFVTFDRSGE
                     ILISVSIDGTIKFWDIQSGNIIETYEFTPRDVADIENVYAMPDDETFLFVKSNQTTIV
                     IDSKRRKHIGYISGKPIWFNIEYGRKNLATIDNKSIVQWQIINQKISIIRSVRGHKDR
                     ISSHEFLDNSIITTSYDKTIKLWDFETLNAITFSGHNAPVIGAILKDTTKIVSADESG
                     VIIYWDIATQKQLAAIYPVGDSLTVVVTPEGYFDGSPKALKYLYYVQGLDIIPLEAYY
                     EQFYRPNLLQRILRGESIEKSKVDFNKRKPNSGVKITEPSQGDYRSINMPVPTNANLL
                     KLKASFTDNGGGVDEVRVYRNNKLVQSEVIGLNKPGESVSRSFDVPLMPGINTITVSV
                     FNSDRTEICDTIKMNFSGTITETPNLYLVCIGINEYENPQYHLNFAVSDVLSFSQAIE
                     TGAKTLFGNIYTYNITDRDATKANITKTLSEVQSKAKPWDVFVFYYAGHGAAISEDGR
                     TNFYFISSDVTNIYSKEQLTGKAISNSELLFFSRDIQAEKQFLVIDACNSGAAGDLLA
                     YRSGPEEQRALAVLARSTGTHFLFASTADQLAKEIPQIGHGVFTYAILQAMTGGEGYF
                     KSDAGVSVKDISNYTERKVPDISQTYIPGGVPQYPTAYSYGQDFPLVLASQTPGVTKL
                     KGKYDDYSIEQLQELKAKAIEEEDFLKANEIKTEIEKRNKN"
     gene            complement(7625..>8186)
                     /locus_tag="PLK75_11150"
     CDS             complement(7625..>8186)
                     /locus_tag="PLK75_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39844.1"
                     /translation="SANEKENFAWYADNLHSKEKSWQRVQELTLPFERKIFDEVENLL
                     MPRWRNADWNNKKAVEELGRQMNRYQNQTAADAFTLIRFLYRPHSEKLQMAGITYRIE
                     SAQVQALLCSNKPIASAKSIDGRKLVLNKPWLQYNKIQVNYGDGSLATESLQHTYAQA
                     GTFLVSVSITTTLGIGISGWVWVDVL"
BASE COUNT         2365 a         1775 c         1582 g         2464 t
ORIGIN      
        1 gatgacctcg tccagctcgc cgggcatcag cctgaagcag gaaggcatca gctatatagc
       61 aggggcagaa ttaccttgtc ttatagttaa tgtggttcgc ggaggtcctg gtctgggaac
      121 catccaaccg agccagagcg actatttcca ggcagtgaaa ggcggtggac atggcgatta
      181 taaactcatc gttcttgcac ccgcatcggt acaggagatg tacgattttg taagcctcgg
      241 gtttgaagtg gcaatgaaat accgcaaccc ggttatgata ctctccgatg gtgttatcgg
      301 ccagatgatg gaaaaagttg agctcggccc tcagaaaaac cgcagatcac gcgaagaagt
      361 tatcaaatcg gcaccttggg caaccacagg gaaacctgct tcgagaccgc ataacattat
      421 cacatccctt gatctgattg ctgcaaatca ggaaaaattc aataaaaaac tcatcgaaaa
      481 atataaaacc atcgaacaaa acgaagttcg cttcgaggag ttatatatgg atgatgctaa
      541 atatgctttt gttgcctacg gttcaagcag tcgtatctgc cagagtgcca ttaagattgc
      601 acgcaaacag ggtataaaag ttggtctcat caggcccatt accttattcc cgttcccgaa
      661 aaaacaaatt gtaaaacgcg ctgaaaaggt taaagggttc ttatcggttg aaatgagtgc
      721 aggccaaatg atagaagatg taaaacttgc gctcaacgga atacgtcctg ccgagcattt
      781 cggaagaaac ggcggtattg tacccacacc cgaagaagtt gtcgaagctc ttaaacataa
      841 ttttatagga ggataaacca tgtcggaaat aacattaaaa gatattataa aagaagaaaa
      901 ccttgtttac agcagaagca aagtaatgac tccgaacgag ttatcgtttt gcccgggatg
      961 cggacatggt actgcccacc ggcttattgc cgaaaccatc gaagagatgg gaatacaaag
     1021 cgaagttatc ggcgtgtcgc cggtaggttg ctctgtactg atgtataact ttatggatgt
     1081 ggatatgcag caggctgccc atgggagggc tccttcactg gccacagcta tcaaaaggct
     1141 gatgcccgat aaatacgttt tcacctatca gggcgacggc gaccttgcag ctatcggaac
     1201 cgctgaaacc atccatgcct gcaatcgtgg cgaaaatata accatagtat ttgtaaacaa
     1261 cggtatttat ggtatgaccg gtggtcagat ggctcccact accctgcccg gtatgcgttc
     1321 atcaacctcg ccctatggac gtgatgtttc gatgatggga aacccgctta agatgacaga
     1381 actcgttgct cagcttgccg gaacctatta tgttacccgt cagtcggtac atacaccacg
     1441 caatgtacgc agggcaaaaa aagccatccg catcgctttc gaaaatcaga aactcaacaa
     1501 agggctttcg tttgtcgaaa ttgtttcgaa ctgcaactcg gggtggaaac tccctcccgt
     1561 aaaagccaac gaatggatgg aagagaatat gttcccgttc tacgaactgg gcgatattaa
     1621 agtggacggt aaattagtaa ccaaataaaa ttcagaaatt atgaccgaag aaataattat
     1681 agccggtttt ggcggacaag gtgtgctctc gatgggtaaa attcttgcct actctggcct
     1741 gatgcagggt caggaagttt catggatgcc ctcatacgga cccgaaatga ggggtggaac
     1801 ctctaatgtt accgttatca tcagcgacga acgtatcaat tctcccattc ttacccaatt
     1861 cgatacagcc attgtgctga accagccttc gatggataaa ttcgaaaaca tggtaaaacc
     1921 gggtggcttg cttctgtacg atccgaatgg gattaccagc ccccctaccc gtaaggatat
     1981 aaacatctac aaggtagaag ctaccgacac tgccgttaaa atgggtattg ccaaggtatt
     2041 caacatgatt gtactgggca gttttatcaa aataaagccg attgttaaaa tcgaaaacgt
     2101 gatgaaagga ctgaagaaat cgctcccgcc acgtaaccac aacctgttac ccatgaatga
     2161 ggatgccatt aaaaagggcc tcgaaattgt ggaagctgtg aacgtactgt aacgatttaa
     2221 cttataatat tgaaaaactc cggtgttgaa ccggagtttt ttgtttggca ttattattct
     2281 aaagaaaagt caatttttat ttctcttttc gatttctgtt tttatttcgt ttgccttcag
     2341 gaaatcctcc tcctcaatcg cctttgcttt caactcctgc aactgctcaa tactgtaatc
     2401 atcatacttc cctttcagtt ttgtaactcc gggtgtttgc gatgccaaaa ccagcggaaa
     2461 atcctgcccg tagctgtaag ccgtggggta ctgtggcaca ccgccgggga tataggtctg
     2521 cgatatatcg ggaaccttgc gctcggtgta gttcgagatg tctttcacac tcacacctgc
     2581 atcgcttttg aaataccctt cgcctccggt cattgcctgc aaaatggcgt aggtgaacac
     2641 tccgtgccct atctgcggga tttcttttgc caactggtcg gcggtgctgg caaacaaaaa
     2701 gtgggtgccc gtgctgcgtg ccaatactgc aagtgcccgt tgttcttcgg gtccgctgcg
     2761 gtaagccagc aaatcacccg ctgccccgct gttgcaggca tcaatcacca aaaattgctt
     2821 ctcggcctgt atgtcgcgcg agaaaaacaa caattcgctg ttcgaaatgg ctttgcctgt
     2881 gagctgttct ttgctgtaaa tgttcgttac atcgcttgag ataaaataaa aattcgtccg
     2941 cccgtcttcg cttatggcgg ctccatgacc ggcgtagtag aacacaaaca catcccaggg
     3001 tttggctttc gattgtacct cactaagagt tttcgtaata tttgctttgg tagcatcgcg
     3061 gtcggttatg ttgtatgtgt aaatgttccc aaacaaggtc tttgctccgg tttctattgc
     3121 ctgagaaaaa ctcagcacat cgctcactgc aaaattcagg tggtattgcg ggttctcata
     3181 ttcgttaatg ccaatgcaaa ccagatacag gttgggtgtt tcggtaatgg tacccgaaaa
     3241 gttcatcttg atggtgtcgc atatttcggt acggtcgctg ttgaataccg atacggtaat
     3301 ggtgttgatt ccgggcatga gtggcacatc gaagctgcgc gaaacgcttt caccgggctt
     3361 gttcagacca atcacttccg attgcaccag tttgttgttg cgatacacgc gaacttcatc
     3421 caccccgcca ccgttatctg tgaagctggc tttcagtttg agcaggttgg cattggtggg
     3481 caccggcata ttgatgctgc ggtaatcgcc ttgcgacggt tcggtgatct taactccaga
     3541 attgggctta cgcttgttaa aatcaacttt cgatttctct atactctcac ccctgagaat
     3601 gcgctgcaat aagttcggcc tatagaattg ctcgtaatat gcctccaaag gaataatatc
     3661 caatccttgc acataataca aatactttaa agctttgggt gagccatcaa aatagccttc
     3721 gggagttacc accactgtta aactatcgcc aaccggataa atggctgcca gttgtttttg
     3781 ggtggcgatg tcccaataga tgatgacgcc ggattcatcg gctgacacga ttttagtggt
     3841 gtcttttaat atagcgccta taacaggtgc attatgaccg gaaaaagtaa ttgcatttaa
     3901 tgtttcaaaa tcccataatt taattgtctt atcatagctt gttgtaatta tgctattatc
     3961 caaaaattca tgtgaagaaa ttctgtcttt gtgccccctg acacttctga ttattgatat
     4021 tttctgattt ataatttgcc attgtacaat gcttttattg tcgattgtag caagattttt
     4081 tcttccatac tcaatattga accaaatagg tttgcccgaa atataaccga tgtgttttct
     4141 tcttttggaa tcaattacta tagttgtctg atttgatttt acaaatagaa aagtttcgtc
     4201 atcaggcata gcataaacat tttcaatatc tgcaacatcc ctaggggtga attcataagt
     4261 ttctataata tttccgcttt gaatatccca aaacttaatt gttccatcaa ttgaaacaga
     4321 aatcaagatt tcgccacttc tatcaaatgt aacaaaagac acataatcgt tgtgtccatt
     4381 taaaatacat cgaatttgtt cactttgcac atcccaaact aaaataccaa aatcactata
     4441 tgcatacatt gctgacaggc tttcagtcgg taaatcccaa tttaaaatgc ttctatcgct
     4501 gtacgccatt gctaataggt tttcgttcgg agaaaagcaa atatcattac ttgttccttg
     4561 tttccaatgc tgatcgtcaa gctgaaattc ttttacagaa tttccggact caacgttaaa
     4621 aattttataa gattgagaac cacatgatgc taaatacttc ccatcatagc taaataggat
     4681 acgtctcaca taattatcat ggcccttaaa ttcttttgac ataatacctg tttgcacctc
     4741 aataagctcg atggtattag agttgttcac ttttgcaatc agcgtaccgt cgggagaaac
     4801 attatcattc cagccaaagt tatcgcttga aacaccatct aaatgattga taaaattgcc
     4861 atttgtaaca ttcaacaatt tcagctcatc tgaagctgta tgcattgcaa gcatatcaaa
     4921 ttgcttaaaa tatgaaacag aattaacaaa aaatccattc gatttattat tattcagagg
     4981 aatgttgttt tcaatatctt ttatttcgat attgttgtca gcaatttgca catagctcat
     5041 aaaatctggt gatataaagc cttcaaagcc attggagctt ttactatcaa cttctttgcc
     5101 acttatcaaa tcccaaaggg caaaactatt tgcagagatt tctgcaataa actcaccatt
     5161 ttgtgaaaag cacaaagatc cataattctc tttaagattg aaattatgta ttaaactgtc
     5221 attttgtaaa tcccaaattt ttaaggtacc gcaactgctc tttgaagcca attttttacc
     5281 atccggcgaa aagcacacat aaaaaaaatg ttcagtatta ccttggatag tatttattag
     5341 cattccattc tgaacatccc aaagtttaat agttgaatca tcgctccctg aggccaacaa
     5401 ttttccatcg ggcgaaaaac aaacagagtt aacaaaattt gtatgacctg agagatttaa
     5461 tagaagtgca ccggtttgca cagcataaag ctttacatca ttatcagaat caaatactat
     5521 tttcgtaccg caagggctaa aacggaaaga gtaataatcc tcagaatatg tccacaacaa
     5581 atttccgttc tgaacatccc aaagtttaca ataagcatta tcatcaatag ttgcaatgta
     5641 tcttccatcg ggtgaaaatg caatcgattc aaatgaacca ttgttctctc caaatgtacg
     5701 taacaaaaca cctgaacata catcccacaa attcatttct cctgtttcat ctgccgatac
     5761 caacagccta ccatcttcag aaaaactaat acaagaaatc tcctgatgct gtccggcaaa
     5821 tgttttgatt aaaactccac tcggataatc ccaaatctta atagtgttat cgtcgctgca
     5881 tgatgccaat aactttccat cggttgagag acacacactt ttcactgtat tgttatgccc
     5941 aatcatcgta gttaaaacag gcttaatcga agataaggta tctgcatttt ggcttataag
     6001 tgctgtaaca atattggttt ggtttcttaa cttagctatt gtatagggag tatatccttt
     6061 agcatcaggc ttgttcatat cagctcccat gtcaataagt tttgcagcaa catctgtatt
     6121 acccaacaat aaagcatata ccaaaagtgt ttgttcctca ctattcgttg tgtttaccaa
     6181 acttttatta gaatataata atttaagaac ttctgtttga ttgtctttaa ttgcattgtg
     6241 aaaaatggtt tggccattgc tgtctttggt atcaacgatt aaaggttttt gagtaatcaa
     6301 atgttccaga cttgtaaaat ctttggccca gtaattcaaa aatatctcgt gatcggtatt
     6361 tgcaagtagt tgaagtacat ctatacttga aaagtttcgg gaaatttcta aagccgacaa
     6421 cccttcgcca ttccataaac ttgtatcggc accattcttt aatagcaaag agacaaattc
     6481 tgcattattg ttcgctgcag caatatgcaa tggggtccat cctaaaaact ccatctgtgt
     6541 atctatacgc ttaggagatt ttgtcctaat attaatattt gcttcatttt caattaggaa
     6601 tttcgccatc ttgtaagaac cattctttgt tgccgcataa tgcaagggtg taattccttc
     6661 gccgtattcc atatcacatg tagtattaat gtcggctcca ttttcaagca ataaatccac
     6721 catttcaaac tcatcattat acatcgaaat acacaatggt gttacattgt atttattttt
     6781 cacattaatc ttggcaccat tatcaataag caattgagta attcggaaat ctgatttttc
     6841 tactgcaata tgcaaagaag tttcaccggt ttttgtcgta gcattcacat ctgcgccatt
     6901 atcaatgaga agtttcacta ttttgctgtt accaatacat tttatagcta tatgtaatgg
     6961 agtgaatccg gcgctctctc taacattttt agaaatctta gcattcacat ttgcgccatt
     7021 ttcaatgagc agttttacga tttcatatcc attattattc tcaacagcaa aatgcaaggg
     7081 agagtaactt tcctttttcc tttcgtctcc cccaagcaag cgaactctgc gatatctagg
     7141 ctctttcatt ttacaagtta gtgcattcac atcggctcct ttttcgatga ggagtttggc
     7201 gatttccagc ccatcttcat ttgccgtcgc aaaatgcaag gacgtgtatc cgttttcatc
     7261 tttcacattc acatccgctc cgtcatcaat aagccttttc gcttcttcgt aattgttgtt
     7321 tttgcaggct tcgaggagtt cgatatcaat actgcgctgt tgtgaaaaca ctgatattga
     7381 tagtatcaga aagactatac aaacgattgg gttcttcatt attgatgatt aattataggc
     7441 taaatttata ataattattt tgattttttt aaagataata tgtacactct ctccttcaaa
     7501 ccccacaaaa atacttaatc aaaaaaagcc tcccaaaccg ttttccacca tcacaaaggc
     7561 aatcgggaaa ttgaaatagt gttgagtttg tttgggttta tagaactaaa attctacatt
     7621 tttttcacaa aacatcaacc caaacccagc ccgaaatacc aatacccaag gtagtggtga
     7681 tgctcaccga aaccagaaaa gttcctgcct gagcatacgt gtgctgcagg ctttctgtag
     7741 ccaaactgcc atcgccataa ttcacctgaa ttttgttgta ttgtagccat ggtttgttta
     7801 gcacaagctt acgaccatca atcgatttgg cagaggcaat gggtttattg ctgcaaagta
     7861 gcgcctgcac ctgtgcgctt tctatacggt aagtaatacc agccatttgc aacttttcgc
     7921 tgtgggggcg atacagaaac cgaatcagcg taaaagcatc ggcggcggtt tggttctggt
     7981 aacgattcat ttgccggcca agctcttcaa cagccttttt attgttccag tcggcattgc
     8041 gccagcgggg catcaacagg ttctcaacct cgtcgaagat tttgcgttca aatggtagtg
     8101 taagctcctg cactctctgc catgattttt ctttgctatg caaattgtcg gcataccatg
     8161 cgaagttctc tttttcgttt gcggaa
//
LOCUS       DAOOQP010000128         7998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11163_length_7998_cov_4.357799, whole genome shotgun sequence.
ACCESSION   DAOOQP010000128 DAOOQP010000000
VERSION     DAOOQP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11163_length_7998_cov_4.357799"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLK75_11155"
     CDS             <1..354
                     /locus_tag="PLK75_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39845.1"
                     /translation="YASYDTQGNIVNDPWPTPFHTCGFDLDAVGVINETSNAIGNVPV
                     QNVSLYPNPANGYVCIQGISEAQRIEICDSAGRLLMVKKNANSFSIETLASGSYIVRV
                     HTLENHVCTLKLIRY"
     gene            323..2320
                     /locus_tag="PLK75_11160"
     CDS             323..2320
                     /locus_tag="PLK75_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY39846.1"
                     /translation="MFVPLNLFAINVNRIYIALLCLLFGFTVKAQDVYLDDSITMLPN
                     VNILAERPVYEHGLKISHIDSLIYEENRQYSMSELIAWKTPVFIKSYGRGSMATASFR
                     GTDPSHTQVTWNGVPLNSPLLGMADFSQIPLIIADGIDLYHGTASLLSGYPAIGGRIS
                     MTGSPVWNKGFELDVISAAGSFHTFDESLQAGYSSKKLSAKAGMWYSYSRNDFGFQNR
                     DVLDGVYQVRENASWKRYGLIADSYFRLRENSVLSVKLWGQSGSRYIPQLSTNESGYQ
                     NNLNRQTDELLNGIISFSSYNNNSRLTVQSAVSMQDCHYTLENYISGTGFLSAVSSVS
                     TVFSSYNFADYKVNFSKKWFASALIQFDFHAVESKEDISVQQYNVRRGEGAASVAVSY
                     KPVENLTVGVSLRQQLTGNRFLPLMPSLQLEFNPIGSLILKSNISRVFRSPSLNDLYF
                     QPGGNPFLLPEESVLTETGIQWSYAHNKVQLQTEITGYYSDIRNWIMWKPTLRGYWTP
                     ENVAKVQSYGIEASLKTEIQVHSFTVSLNSGYTLSHSVNKTVPENAGDLSYGCQLPYI
                     PVHSAMLYGGVSYKGFTFGYQWNYYSERLTSTAGESSPLISLYPYFMNDVSLGKHFDM
                     NAYSINLRFSVMNLFNESYRSVLWQPMPGRNYMLTLRFMLK"
     gene            2371..3375
                     /locus_tag="PLK75_11165"
     CDS             2371..3375
                     /locus_tag="PLK75_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOY39847.1"
                     /translation="MHDDVKLNTYNTGSGVFVVCSGNFMYGNSSLSFYHPSTGDVNNE
                     VFFNANGFPLGDVAQSLILTDSLGYIVVNNSGRIYAFDPNSFIAKGKIIGLTSPRYIH
                     LLNAEKAYVSDLYAKKIWVVNPINLSVIGSIDVDNHNNEFYQHSTEQMVQYGKKVFVA
                     CWSFDNKLLVIDTENDRLCDSITVGLQPAKMLIDRYHQIWVYCDGGSEGAPFGHESPS
                     LYRVNSETLEAVKVFEFDTGVFGAAMCMNANGDTLFFTACDVYAIDVLSPTMPEQPFV
                     EAGARNITAIACDAATGDFYMADAVDWMQQGIVYRYSRNAHPLDTFRVGINPVEIVFK
                     "
     gene            complement(3383..3760)
                     /locus_tag="PLK75_11170"
     CDS             complement(3383..3760)
                     /locus_tag="PLK75_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOY39848.1"
                     /translation="MDKRNLIKYLLIAAGIFSTALGVIGIFVPLLPTTPLLLLAAACF
                     MRSSDRLYTALIRHKVLGIYIKNYMDKKGIPIRIKLFSITILWAGILSSAVFATDKLW
                     LRILLVTIAIAVSIHIILIKKKD"
     gene            complement(4307..5650)
                     /locus_tag="PLK75_11175"
     CDS             complement(4307..5650)
                     /locus_tag="PLK75_11175"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39849.1"
                     /translation="MKKLLFTFGFITFCMFAFAQQEDITEDIQTLKEKAKQGSAEAQG
                     KLGYCYITGTNTEKNEKEGFRLLTESVKEGIAFSQYYLGQCFQKGIGTSQNDVKAFQW
                     TLKAGEQEFTKAFPLLGVWYYNGIGTSKNTKEAAKWLKKAVDIDNDTESMYYLAMCYK
                     TGDGVFINEGKAVEYFAKAAEKGHAQAQNELAYAYRYGKGVTQNSAKAVELYNSIAEK
                     GDKEAQYYLATCYEKGDGAVKNETTAFGWYLKAAENGHVLAQNSVGACFEKGIGTSVN
                     YDKAFEWYKKATDAGNLDAMNNLGNCYYNGYGTKTDYLKAFELYTKASKNRMPAALYN
                     MGVCYSKGNGVKLNTKKSSELLFEAAQANYTPAQNLLGTFYLEGIGVAKDLKKATTWF
                     SIAAEAGNADAQYNLGNCYRTGIGLPKDANKAREWLSKAAAQGHEQAKKDLEKIK"
     gene            complement(5872..7182)
                     /locus_tag="PLK75_11180"
     CDS             complement(5872..7182)
                     /locus_tag="PLK75_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOY39850.1"
                     /translation="MLTNEGLRRDVGLWGLTANIVNVVIGSGIFVLPALVAAGLGAAG
                     ILAYLFCGFLISIIMLCFAEVGSKITVTGGAYAYIEAAFGKYFGFLAACLLIFGACLM
                     ATAAVANAMADMLSCIWPVFSNQSFRVFFFVALFSGFTIINIVGIKQGITLIIITTIV
                     KLAPLLLLVIWGSTEIVPSNLLWHGFPAVSDIGKVSLLLFFAFQGAESSLSVSGEVKN
                     AHKTIPKGILLSFLIILVFYISIQLVAQGVLGNAFAGFSEAPLAEVASRLMGPAGLTL
                     MIAGASVSMFGYLTSDTLNIPRVLFRSSIDKVIPVKPLAMVHKRYATPYIAVISYSAL
                     GCLFAITGEFKQLAILSSASVLLVYLGVSLSVIKLRRSVKPEHRTFRIPGGYAIPILS
                     SATIIWFLSNLTHQEMAGMGIALVLLSITYIAIRLLRKQSQLKS"
     gene            7486..7953
                     /locus_tag="PLK75_11185"
     CDS             7486..7953
                     /locus_tag="PLK75_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY39851.1"
                     /translation="MKTSMKIPEREPIGRVMARAGKLFLTEIRNQLPDISINRSFYPL
                     MLIYSGNGELTQQELANLLKIDKVQVVRIVDYLSQHGYVERINNSSDRRKHLLCTTSK
                     ATIAMPFILAAFESVTQMAMKGLSSEQKDMLYQSMGIIEANLISNRKIKTNES"
BASE COUNT         2266 a         1637 c         1681 g         2414 t
ORIGIN      
        1 tatgcaagct acgatacaca gggaaacata gtaaacgacc catggccaac gcctttccat
       61 acctgtggtt tcgacctcga tgcagtaggt gtgatcaatg agacgagtaa tgctattggc
      121 aatgttcctg ttcagaatgt ttcgctttat cctaacccgg ccaatgggta tgtgtgtatt
      181 caaggcatat ccgaagctca acgcatcgaa atatgcgata gtgcagggag attgctgatg
      241 gtcaaaaaaa atgcaaactc tttttcgata gaaacacttg cttcgggtag ttatattgtc
      301 cgggttcaca cccttgaaaa ccatgtttgt acccttaaac ttattcgcta ttaatgtaaa
      361 tcgtatctat attgccctgt tatgcctgct gttcggattt accgtgaaag ctcaggatgt
      421 atacctcgat gactcgataa ccatgctccc caatgtaaat atactggcag aaaggccagt
      481 atatgaacac ggacttaaaa tatcgcatat cgattccctg atttatgagg aaaaccggca
      541 atacagtatg tcggagctga tagcatggaa gacgcctgtt tttattaaat cgtacggtcg
      601 tggaagtatg gctacagcgt cgtttcgggg aactgaccca tcgcatacac aggtaacatg
      661 gaacggagtt ccgctgaatt ccccgttact tgggatggcc gattttagtc agatacccct
      721 gattattgca gatggcattg atttgtatca tggcactgct tcgctgttgt cggggtaccc
      781 tgccattgga ggcagaattt cgatgaccgg cagtccggtt tggaataaag gttttgaact
      841 tgacgtgatt tcagctgccg ggagctttca cacttttgat gaatcgctgc aggcagggta
      901 ttcttcaaaa aaactgagtg caaaagcagg aatgtggtat tcatattccc gtaacgattt
      961 tggctttcag aaccgcgatg tgcttgatgg tgtttatcag gtcagggaaa atgcctcatg
     1021 gaaaagatac gggcttattg cagattctta cttccgtctc cgcgaaaata gtgtgctttc
     1081 ggtaaaactc tgggggcaat ctggaagcag atacattccg caattatcca ccaacgaaag
     1141 tgggtatcaa aacaacttaa accggcagac tgatgagctg ctgaacggaa taatttcttt
     1201 cagcagttac aacaataact ccagattaac ggttcagtct gctgtttcga tgcaagattg
     1261 ccactacaca cttgaaaact atatcagtgg aaccggattt ttgagtgctg tcagcagcgt
     1321 aagtacagta ttttcgagtt ataattttgc cgattataag gtaaatttta gcaaaaaatg
     1381 gtttgcttca gccctgatac aatttgattt tcatgctgtt gaatcgaagg aggatatatc
     1441 ggtccagcaa tataatgtga gaaggggaga aggtgcagca tcggttgcag tatcctataa
     1501 acctgttgaa aatttaaccg ttggagtatc cttacggcaa cagcttaccg gtaaccgttt
     1561 ccttccgcta atgccttctt tgcagcttga attcaatccg attggttcgt tgatcttaaa
     1621 atcaaatatt tcgcgtgtat ttcgcagccc ttcgctgaac gatttgtatt ttcagccggg
     1681 tggcaatcca tttttattac ctgaggagtc tgtgctcacc gaaacgggaa tacaatggtc
     1741 gtatgcacac aataaggtcc aactgcagac cgaaattaca gggtattaca gcgacatcag
     1801 gaactggatt atgtggaagc cgaccctaag aggctactgg acacctgaaa atgttgcaaa
     1861 agttcagtca tacggaatag aagcatcact gaaaacagaa atacaggttc attcattcac
     1921 agtgtcactg aacagcggat acacattgtc gcattccgta aataaaaccg tacctgaaaa
     1981 tgcaggagac ctttcgtatg gttgtcagtt accctatatt ccggttcatt cggctatgtt
     2041 atatggcggt gtttcctata agggttttac tttcggctat caatggaatt attatagtga
     2101 acgcctcaca tcaacagcag gcgaatcgtc tcccttaatc agtctgtatc cgtattttat
     2161 gaacgatgtc agtttgggaa agcatttcga tatgaatgca tactcaatca atctgaggtt
     2221 ttcagtaatg aatcttttca acgaaagcta tcgctcggtt ttgtggcaac ccatgccggg
     2281 acgtaattat atgctgactc tacgatttat gctcaaatga gaaaggtgct gttatatatc
     2341 gtttttttgt ttgtgtttat gcactcgtgc atgcacgacg atgtaaaact gaacacatac
     2401 aacactggca gcggggtttt tgtggtttgt tccggtaatt ttatgtatgg caattcttcc
     2461 ttgagttttt accatccgtc caccggcgat gtaaataacg aagttttttt caatgcaaac
     2521 ggatttccgt tgggtgatgt tgcccagtcg cttatcctta cagattcact tggttatatc
     2581 gtcgtaaaca attcgggacg tatttatgcc ttcgatccga attcatttat tgcaaaaggt
     2641 aaaattatag ggctcacttc accgcgttat attcacctgt tgaatgctga aaaagcatac
     2701 gtgagcgatc tgtatgcaaa aaaaatatgg gttgttaatc cgataaacct gagcgttatt
     2761 ggcagcattg atgttgacaa tcacaataat gagttctatc agcacagcac tgagcaaatg
     2821 gtgcagtacg gcaaaaaagt gtttgtggcg tgctggtcgt tcgacaataa actcttggta
     2881 attgacactg aaaatgacag gttgtgcgat tcgataaccg ttggtctgca acctgctaag
     2941 atgctgattg accggtatca ccagatatgg gtgtattgcg atggaggaag tgagggggca
     3001 ccatttggac atgaatcccc ttcgttgtat cgtgtcaata gcgaaacact ggaagctgtt
     3061 aaagttttcg aattcgacac cggagtgttt ggtgcagcaa tgtgtatgaa cgccaatggc
     3121 gatacccttt tctttactgc atgcgatgtg tatgcaatag atgtgctatc gccaacaatg
     3181 ccagaacagc cgtttgttga agcaggtgcc cgaaatatta ctgcgattgc ctgcgatgct
     3241 gcaaccggcg atttttatat ggccgatgca gtggattgga tgcaacaagg aattgtgtat
     3301 cgctattcac gaaatgcaca tccgctcgat actttcagag ttggcataaa tccggtggag
     3361 attgtgttta aataattacg actcagtctt tttttttgat aagtataatg tggatactta
     3421 ccgcaatggc aatggtaacc agtaatatgc gcagccacag cttgtcggtt gcaaaaacag
     3481 cactgctcag gatacctgcc cacaatatag taatcgagaa tagttttatt ctgataggta
     3541 tgcctttttt atccatataa ttttttatgt agatgccaag cactttatgg cgaatcaggg
     3601 ctgtgtacag acgatcgctg ctgcgcataa aacatgctgc agccagcaga agtaggggag
     3661 tagtgggaag tagcggaaca aaaataccaa tcacacccag ggctgtcgaa aatatgccgg
     3721 cagctatgag taggtatttt atcaggttcc ttttatccat gcgccaaaag tactgaaatt
     3781 ggaggatatt gctctgccta cattcgaaat attaccatat tatcgcgggg tggtgaatat
     3841 aaggtttggg gataattctt tatagaatac agaagtcaga atacagaata cagaaggggc
     3901 gatttttaac gtttgtcacc ctgagtagcg aagcggtaat cgaagggtga atccgaagac
     3961 tttttccatc cccctgtcta tgctacgatt aaccttctgc tacgctccat tactcagcat
     4021 gacaggggaa tttgtcaccc ttagtagtgc agcgataatc gaagggtgaa tccgaagact
     4081 ttttccatcc ccctgtctat gcttcgatta accttccgct acgctccatt actcagcatg
     4141 acaggggaat ttgtcaccct tagtagtgca gcgataatcg aaggctgaat gcgcaaaggt
     4201 ttatcggttt ttcctcttgt agtctgtatc cccaacatta atatgatacc aaaaacaaca
     4261 aaggctgcct gtgattgaca gcctttggtt tgtatagaat tcagcactat ttaattttct
     4321 ccagatcttt ctttgcctgt tcgtgcccct gtgctgcagc ttttgagagc cattcacgtg
     4381 ctttattggc gtctttgggt aatcctatac ctgtacgata acagtttcct aagttatact
     4441 gagcatcggc atttcctgct tcggcagcga tgctgaacca tgtggttgct tttttcagat
     4501 cttttgcaac tccaatacct tcgaggtaaa acgttcccaa caggttttgc gccggagtgt
     4561 aatttgcctg tgcagcttcg aaaagtaatt cagatgactt ttttgtatta agcttaactc
     4621 cattcccttt tgaatagcaa acacccatat tatataaagc tgctggcatt ctgtttttcg
     4681 aagcttttgt atagagttcg aaagctttca gataatctgt ttttgtgccg tatccattgt
     4741 aatagcagtt tcccaggtta ttcattgcat caagattacc ggcatcggtg gcttttttat
     4801 accattcaaa tgctttatcg tagtttactg atgttccaat tcctttctca aagcaagctc
     4861 ctacagaatt ctgtgcgagt acgtgcccgt tttcggctgc tttcagatac cagccgaatg
     4921 ccgtggtttc gtttttaaca gcaccatcac ctttttcata acaggttgcc agataatact
     4981 gggcttcttt atcgcctttt tctgctatcg aattgtataa ttccacggct ttagcagaat
     5041 tttgtgtaac tcctttgcca tatctgtatg cgtatgcaag ctcattttgt gcttgggcat
     5101 ggcctttttc agcagctttt gcgaaatatt caaccgcctt gccttcgtta ataaaaactc
     5161 cgtcaccggt tttatagcac atggccaggt aatacatcga ttcggtgtcg ttgtcgatat
     5221 ctactgcttt ttttagccat ttggccgctt cttttgtgtt tttacttgta ccaattccgt
     5281 tgtaatacca gacaccaagc aaaggaaatg ctttagtaaa ttcctgttcc ccagctttta
     5341 atgtccattg gaatgctttt acatcgtttt gagatgtccc gattcctttc tgaaaacatt
     5401 gccccagata gtattgcgaa aaagcaatac cttctttcac cgattctgtc aacagtctga
     5461 atccttcttt ttcgtttttc tcggtatttg ttccggtaat gtagcagtag ccaagtttcc
     5521 cttgtgcttc ggcactcccc tgtttagctt tttctttcaa tgtctgaatg tcttcagtaa
     5581 tatcttcctg ctgtgcaaat gcaaacatac agaacgtaat gaaaccgaaa gtaaatagta
     5641 gctttttcat attgtggttt ttagttaata ttctgctttt ctaaatggtt gttatgttac
     5701 aaatttatga aaaacttatt ttgtattcaa ctatttgttt aaaatcttga aattttattg
     5761 actttatatc cctcttacaa aatacgtgca aaatttactg agtttaaatt atgtttaaac
     5821 accaaaaaaa aacagcagac attacatatt cggataaaaa aaaagtacgt tttatgattt
     5881 taattgtgat tgcttacgga gcagacgaat agcaatgtag gtaatgctta acagaacgag
     5941 tgcaataccc attcctgcca tttcctgatg tgtgaggttc gatagaaacc atattatggt
     6001 tgctgaggat aatattggta ttgcatatcc tccgggtatt ctgaaagttc tatgctccgg
     6061 ttttactgaa cgtcgcaatt ttattaccga aagggaaacc cctaaatata ccagtaatac
     6121 cgaagcggac gaaagaatag caagttgctt gaattcgccg gtaatggcga aaaggcatcc
     6181 cagggctgaa taggatatta cagcaatata aggggtagca tatcttttgt gaaccatagc
     6241 tagcggtttt actggaataa ctttatcgat agacgatcgg aacagtaccc gcggaatatt
     6301 cagtgtgtcg ctggtaaggt atccaaacat cgaaacggag gcccctgcaa tcatcaatgt
     6361 taaccctgct ggccccatca gtctgctcgc aacttcggca agaggcgctt cggaaaaacc
     6421 tgcaaaagca ttgcccagca ccccctgtgc aaccaattga attgaaatat aaaataccag
     6481 aataataaga aaactgagta aaatcccttt gggtattgtt ttgtgtgcat ttttaacctc
     6541 tccgctcacg ctgaggctgc tttctgctcc ctgaaaagca aaaaatagca gtagtgaaac
     6601 ttttccgata tcggaaactg ccggaaatcc atgccagaga aggttggagg gaacaatctc
     6661 agtagatccc catattacca gcagcaacag gggtgcaagc ttaacaatgg tcgtaatgat
     6721 aattaaagta atgccctgtt ttattccaac aatattaatt attgtaaaac ctgaaaacaa
     6781 agccacaaaa aagaaaactc tgaatgattg attgctgaaa acgggccaaa tacacgataa
     6841 catatcggcc atagcatttg caacagcggc agttgccatc aggcatgccc cgaaaataag
     6901 caggcaggca gctaaaaacc caaaatattt tccgaatgct gcttcaatat aggcataggc
     6961 accaccggtt accgtaattt tacttccaac ttcggcaaaa caaagcatga ttatgctgat
     7021 gagaaatccg caaaacagat aggctaatat tccggctgct cccaaacccg ccgctaccaa
     7081 tgcgggcaaa acaaaaattc cggagccgat gactacgttt acaatattgg cggtcaggcc
     7141 ccatagccct acgtctctcc ttaacccctc gtttgtaagc atctgcaatt attcgggttg
     7201 ttgttgattg taccagtata tcgggtaaaa ttataaatat tttctgaata tgaaggggct
     7261 tcaatttatt aaaacagttt taccaggtac gaaaaagtat tgcagagtta cctgctggat
     7321 gctttttctg aaggaggctt tatggtaatg aacccgttgt atatctgtat ttaagaatag
     7381 gcatctatta tccgcacagg cattagtaaa aatttatctt aaaaaatcgt taacattgct
     7441 aactatttac aaatataact aattttatcc cttagaatcc gcgttatgaa aacatcaatg
     7501 aaaattccag aaagggagcc tattggaaga gtaatggcac gtgccggaaa attgtttctt
     7561 accgaaattc gtaatcaact tcctgatata tcaatcaaca ggtcttttta tccgctcatg
     7621 ctaatttatt ctggtaatgg ggagctaacg cagcaggagc ttgcaaacct tctcaaaatc
     7681 gacaaggttc aggtggtaag gattgttgac tatttgtcgc agcacggtta cgttgagcga
     7741 attaataata gcagtgaccg gcgcaaacac ttgctttgta ctaccagcaa agctactatt
     7801 gcaatgccgt ttattcttgc agcattcgaa tccgtaaccc aaatggcaat gaaaggactc
     7861 agcagtgaac agaaagatat gctttatcaa agcatgggta taattgaagc aaaccttata
     7921 tcaaatcgaa aaattaaaac aaatgaaagc taatatagtt gcctggatta tcgcggtaat
     7981 tgtggctgct ttacttgt
//
LOCUS       DAOOQP010000129         7975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11205_length_7975_cov_4.952399, whole genome shotgun sequence.
ACCESSION   DAOOQP010000129 DAOOQP010000000
VERSION     DAOOQP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_7975_cov_4.952399"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLK75_11190"
     CDS             <1..604
                     /locus_tag="PLK75_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOY39852.1"
                     /translation="IIALGKQFPGKKANRFIEWFTYSDESIDGKSKTKAYALFESSFI
                     DSIEVGTSKGLQQIHAYLFGGLYDFAGQIRQKNISKGGFQFAVSHFLGETLQQIERMP
                     ENSFDEIVEKYVEMNIAHPFMEGNGRSTRVWLDLIMKKRIKKCIDWSKIGKADYMNAM
                     ILSSQDDSTLKQLLKNALTDQINSREMFMKGIDYSYYYEE"
     gene            complement(784..1914)
                     /locus_tag="PLK75_11195"
     CDS             complement(784..1914)
                     /locus_tag="PLK75_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39853.1"
                     /translation="MRKIVLLIVLSIFGLSMQAQTSTSKGGWKMVNYSFIDGTKTVDR
                     VLAGTSDYMEDVTKYEGSKGDIIIHFNRYDKSSGKLLAGVDYNVQWTNPESEIFPGDF
                     IRIKYTLKTMSSKTWTPDQQSIYFNQGYNGIYMTNDLGEKYFKKDFSGVLSGKIAVDK
                     GVKAGQERTITINMGAGFKAIYSYVWNPELVKTSTQENLKEENSVADDKTGWYFTRWE
                     YIVSTSDGTKTGKFANGDTYSEVNTATGDKNNFTTSVTRIDKNGKTIASGKAVTRWTD
                     PPKYFSDIELPSIEVNRAVVSSWGISQFSVSFDMKDINPGGGTAGKINFATADAKTHI
                     QAFEGTMKAPKISKGNKHGEQKAIIFHLNGYGFKYYYEWRVY"
     gene            complement(2105..3697)
                     /locus_tag="PLK75_11200"
     CDS             complement(2105..3697)
                     /locus_tag="PLK75_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY39854.1"
                     /translation="MNKALLYIIGLLIFSSASAQQLSLTDFYFVNAYYLNPAAAGENG
                     TNLYLQNRQQWKQIPGAPETTALTFDTKINGRNVGVGINLSIDADNIFRKTMLGASYS
                     YSVRFGEKHYLSMGLSLNVVNTRVAYDQIESADWSDPLLFASSKSVSNISGDAGLNYR
                     FNNLHIGLAGYQLPGTRMLFQSNGNVQQVSYQLVQHFWGVINYEFHLLNNKLSISPEI
                     QARTTIGLPLQLDAGLNFEVLSVAQARLAYRHNSSVYLALAFDIYDNLSIGCAYDYSV
                     GAIADFSGSTYELIIGYKIIRNQNKETNSAENRKLDKSMKRNMQEQSEQLDKLEYENK
                     SMKDEIDDNKEQITILKEEIEQLKKCAELSDEDMKLLNDIKKNHEFVDTDYNSQDRSN
                     DANNNSDSIHQNPTLNNNFNKTDNSNSQDSRNNRNDNEISAINSKQEVTEDISGQYCV
                     IVGAYRDINYAKQGQKILQREINLETTLIREETGNFYFICSNFFDSKQEVQAEKERLH
                     LLNIEDYIVGKPWTYVKFTKNN"
     gene            complement(3704..7057)
                     /locus_tag="PLK75_11205"
     CDS             complement(3704..7057)
                     /locus_tag="PLK75_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39855.1"
                     /translation="MNDFSFKSVIVMLSFLLWSTATYSQVSDGGTAVASPGTVCADVN
                     TGTIALMSSVGDVLYWEYSISGSDPWITISYQSKPLPFNNLQQTTYFRAIVKFGATDQ
                     DTSTVAVVHVSSLTQAGNLVGNMELCGSGNSGTLNLSNYNGNITRWEYSNNEGVSWNT
                     IANTNPSYSFNNLSSTNWYRTVVQSGACSEAISNTAVVTIYPPTQTGTLEGNNDVCAL
                     SNNGQLIVSGHTGDLVGWESSQTGFSPWSSIDYQNDTLEYTNLLQTHHFRALVKSGVC
                     QSIYTNTVEVYVSPVSHGGIVSGHQEVCAGNNSGTLVLSDYTGTIMKWQYSFDYGTTW
                     NDTANATHTLNFSNLNQTTMYRTVIQSGACAAAYSEICTIVVHPIPDVGFTYDTVCYS
                     HPTSFVNSSSISAGSIVSYSWDFGNGDGNSSTNPVYIYPSYGMFTVKLVAVSNHSCID
                     SISAVVPVYPSPVVSFYAANKCHADTLFCVNNSFAPNGGALAYEWNFGDGSMSDIENP
                     NHVYPAAGNYAVSLVVTQIESGCSDSVKHNIEIFPRSIPNFITQNQCDGTPLSFNNTT
                     YIEYGSAMSFWAFGDGNFSNEINPVHLYNSFGEYVVTLTATTNQGCVDTVSKIIHVHP
                     QPIANFSFDDVCFTDSMYFVDNSILPQGDLYYHWNFGNSDNSNEVSPTYYYQAPGTYS
                     VTLSVNTDSACQSSISKLVNVYALPNVNFVANNECLYNEVNFENLSVIQQGSLEYVWD
                     FGDGNNSDDVEPLHLYNSAGTYVVKLIATVNNMCADSVSKQVVVYPIPEPMFSAENVC
                     DGEMSYFFDESHIESGSIANYSWDFGDGTNSVQQNPVKQYLNPGTYQVVLSVTSNFSC
                     QHTYSSFVTVDYMPLANFSVSDVCDGIPINPQNQSIIQAGNMFYQWSFGDMEISIVPN
                     PNHIYNTHGIYRLVLQVQSENGCTDSLARYVQVFALPNADAGQDVVISKGDEIRLSGS
                     GGALFTWFPSSFLSNATIANPVANPLETIEYILQAEDTRGCVNSDTVMIEVLEDFKIK
                     PNNIITPDGNGINDTWYVENIESYGLCEVFIFDRIGNMVFQTKSYANNWKGENMNGDI
                     LPDGTYYYAIVFENSDVVYKGSVSILRNN"
     gene            complement(7061..>7975)
                     /locus_tag="PLK75_11210"
     CDS             complement(7061..>7975)
                     /locus_tag="PLK75_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39856.1"
                     /translation="GNPNCSFLWNTLQTSATIQVVETGNYTVQVTNEFGCSSEDSVAV
                     VFHPNPIVHLGADLYLCANQMALLSAGDAGQYYAWGSDFGFSSFENAVIVADSGKYWV
                     DVTNEFGCTASDTVIVQFSELSLTAYFLAASEAMRGDTIRFVDVSYPEPTHYLWDFKD
                     GVTSTEAIPQHVFYVEGNYLVTLMVSNNICSDTISKPIQISGTSKIYIPKEVENDNTE
                     HFFEILSEKLYPNPNDGSFTYELQLNQHSVISLSLFDLHGKMIYQEQIDHASYVNKSF
                     NFNHLDPGIYILRMNVLNKMKTYKIVIM"
BASE COUNT         2344 a         1583 c         1556 g         2492 t
ORIGIN      
        1 aattattgca ttgggcaaac aatttcctgg taaaaaagca aaccgattca ttgaatggtt
       61 tacatacagt gatgaaagta ttgacgggaa aagtaaaaca aaggcgtatg ccctgtttga
      121 gagctctttt atcgacagta ttgaagttgg aacaagcaag ggattacagc agatacatgc
      181 ctatttgttt ggcggtttgt atgattttgc aggacaaatc aggcaaaaaa acatttcaaa
      241 gggtggattc caattcgctg tatcgcactt tcttggcgaa acattacagc aaattgaacg
      301 aatgcccgaa aattcgtttg acgaaattgt cgaaaaatat gtagagatga acatagctca
      361 tccatttatg gaaggcaatg gtcggagcac ccgagtctgg cttgatctga taatgaaaaa
      421 acgcataaaa aaatgcattg attggagtaa aattggcaaa gccgactaca tgaacgcaat
      481 gattctgagt tctcaggacg acagcacatt gaagcaatta ttaaaaaacg ctttaaccga
      541 ccaaatcaat agccgtgaaa tgtttatgaa aggaattgac tactcgtatt attatgaaga
      601 gtaatgaatt atgaattatg aattatgaat tatgaattat gaatgttaag gattggttat
      661 gaatgaaagg tatattccat taaaagatag gtcttttgca tttgcagtta tacttgtcaa
      721 ttctttcaag gatttgaacg aaaataaata agtagttcgt aatcagcaag cactaaaaat
      781 caatcaatat accctccact cgtagtagta tttaaaccca taaccgttaa ggtgaaagat
      841 aattgctttt tgttcgccat gcttgtttcc cttgctaatt ttcggagctt tcatcgttcc
      901 ttcaaatgct tgaatatgag tttttgcatc agcagttgca aaattgatct taccagcggt
      961 tccgccacct ggatttatat ctttcatatc aaagctaacc gaaaattgag aaattcccca
     1021 tgaagataca acagccctat taacttcaat cgaaggcaat tcgatatcgg aaaaatattt
     1081 tggaggatct gtccatcttg taaccgcttt gcccgatgca atagttttac cgttcttatc
     1141 aattcgagta accgaggtag taaaattatt cttatcgcct gttgcagtat taacttcgga
     1201 ataagtatca ccatttgcaa acttaccggt ttttgtccca tccgatgtgc ttacgatata
     1261 ttcccatcgt gtaaagtacc aaccagtttt atcatctgca acagaattct cctcttttaa
     1321 attttcttga gtgcttgttt ttactaattc tggattccaa acataagaat aaatcgcttt
     1381 aaacccagct cccatattga tagtgatagt tctttcttgc cctgctttca cacctttatc
     1441 caccgcaatt tttccagata agacaccact aaaatccttt ttaaaatatt tttcgccaag
     1501 gtcgttcgtc atataaatac cattataacc ttgattaaaa taaatagatt gttgatctgg
     1561 tgtccacgtc tttgaactca tcgtttttaa agtatatttt atacgaataa aatctcctgg
     1621 aaaaatctca gactcaggat ttgtccattg cacattataa tcaacaccag ccaaaagttt
     1681 accagagctt ttgtcgtaac gattgaagtg aataataata tcacctttac tgccctcgta
     1741 tttggtaaca tcttccatgt aatcagacgt tccggctaac accctatcta cagttttggt
     1801 cccatcaata aaactgtaat ttaccatttt ccagcctcct tttgaagtgc ttgtctgtgc
     1861 ctgcatactc aaaccaaaaa tgctcaggac aattaataag acaatttttc tcatatcatt
     1921 tttttttaag atgtgaaaga tacaaaataa taacgattat ttaaactaat aaacgtataa
     1981 atgctgtgaa atattgcaaa aatagaggtc aattcgacaa gttgtggatg ggtgcacatt
     2041 gtaacaagcg caacgtaaag attattgtat tgaccctatt ggcaaatgat tttggtgcaa
     2101 tcagctagtt gtttttggta aactttacgt aagtccatgg cttaccaaca atataatctt
     2161 cgatgttgag taaatgtaat cgttcctttt cggcttgaac ttcttgtttg ctatcgaaaa
     2221 aattagagca aataaagtag aaattacccg tttcttcgcg aatcagagtg gtttctaagt
     2281 tgatttctcg ttgcaaaatc ttttgacctt gtttggcata gtttatatcg cggtaagccc
     2341 cgactatcac acaatattgt ccggaaatat cctctgtaac ttcttgcttt gaattgatgg
     2401 ccgatatctc attatcgttg cgattgtttc gggaatcctg tgagttgctg ttatctgttt
     2461 tattgaagtt gttatttagt gttgggtttt gatggataga atcggaatta ttatttgcat
     2521 cattactacg atcctggctg ttataatcgg tgtcgacaaa ctcatggttt ttcttaatat
     2581 cattaagcaa cttcatgtct tcgtcactta gctctgcgca tttttttagt tgttcaattt
     2641 cttcttttag aatcgtgatt tgttctttgt tatcatcaat ttcatctttc atagatttat
     2701 tctcgtattc taatttatct agctgttcag attgttcctg catatttctc ttcatcgatt
     2761 tgtcgagttt tctgttttcg gcactgtttg tttccttgtt ttggtttcta atgattttat
     2821 atcctataat tagttcgtag gtagaacccg aaaaatcagc aattgctcct acgctataat
     2881 cgtaagcaca gccaatgctt agattgtcgt atatatcgaa agcaagagcg agatataccg
     2941 agctattgtg cctgtatgcc aagcgagctt gtgccacact aagaacttcg aagttgaggc
     3001 ctgcatcaag ttgtaagggc aatccgatag ttgttctggc ttgaatttca ggacttattg
     3061 ataatttgtt gttcaatagg tgaaattcat aattaatcac tccccagaaa tgctgaacca
     3121 attgatagct tacttgttga acatttccat tcgactgaaa caacattcgt gttccgggaa
     3181 gttgataacc ggccaaacca atatgcaaat tgttgaagcg ataattgaga cctgcgtcac
     3241 ctgaaatgtt actaacactt ttgcttgacg caaataaaag agggtcgctc cagtcggcag
     3301 attcaatttg gtcgtaagcc actctggtat ttaccacgtt gaggctaagc cccatcgaca
     3361 aataatgttt ttcgccaaac ctaaccgaat aggagtagct ggctccaagc atagttttac
     3421 ggaagatgtt gtctgcatca atcgaaagat ttatgccaac acctacgttc cgcccgttaa
     3481 ttttcgtgtc gaatgtcaat gccgttgttt cgggtgcacc tggaatctgt ttccattgtt
     3541 gtcggttttg taaatacagg tttgtgccat tttcaccggc tgctgcgggg tttaaatagt
     3601 atgcattcac aaaatagaaa tcggtaagcg acaattgttg cgccgaagca gacgaaaaaa
     3661 tcaacaaacc gatgatatat aatagagcct tattcataaa caattaatta tttcttaaaa
     3721 ttgaaacact gcctttgtaa accacatccg aattttcgaa aacaatagca tagtaatagg
     3781 tgccatcggg cagaatatcg ccattcatat tttccccttt ccagttgtta gcatacgatt
     3841 tcgtttgaaa taccatattt ccgattctgt cgaaaataaa gacctcgcat aagccataac
     3901 tttcaatatt ttcgacatac caggtgtcgt taatcccatt tccgtcgggt gtaataatat
     3961 tatttggctt tattttaaag tcttcgagca cctcaatcat aaccgtgtcg gagtttacac
     4021 atccccttgt atcttctgcc tgcaaaatgt attcaatagt ttctaaggga ttggccactg
     4081 ggttggcaat agtagcattc gataaaaatg atgatggaaa ccatgtaaat agggcaccac
     4141 cgcttccact caggcgtatt tcgtcgcctt tactgataac aacatcttgt ccggcatctg
     4201 cattgggcaa agcaaacact tggacatatc gtgccagcga gtcggtgcaa ccattttccg
     4261 attgtacttg caataccaaa cggtatatac cgtgtgtgtt atatatgtga ttgggatttg
     4321 gaacgatgga tatttccatg tcgccaaaac tccattgata aaacatatta ccagcttgga
     4381 ttatactttg attttgtgga tttataggaa ttccatcgca gacatcacta accgaaaagt
     4441 ttgccaatgg catataatcg acagtaacaa acgaagagta agtgtgttgg caagagaagt
     4501 tagatgtaac acttagcacc acctgataag ttcccggatt cagatattgt ttaacggggt
     4561 tttgttgcac cgagttggtg ccatctccaa aatcccacga gtagttggca atgcttccgc
     4621 tttcgatgtg cgattcatcg aaaaagtatg acatttctcc atcgcataca ttttcggcac
     4681 tgaacatggg ttcgggtatt ggatacacaa ctacttgttt gcttaccgaa tcggcgcaca
     4741 tgttgttaac cgttgcaatt aattttacca cataagtgcc ggcactattg tacaaatgca
     4801 gaggctcaac atcatcagaa ttatttccgt ctccaaaatc ccatacatat tctaaactcc
     4861 cttgctgaat aaccgacaga ttttcaaaat tcacttcgtt atataagcat tcgttgtttg
     4921 caacaaagtt tacattgggt aaggcataca cgttgactaa tttagagata ctgctttggc
     4981 aggcactgtc ggtatttacg ctaagtgtta ccgaataggt tccaggagct tgataatagt
     5041 aggtgggtga aacttcgttt gagttgtcgc tattgccaaa attccagtga taatataaat
     5101 ctccttgcgg taaaatcgaa ttatcaacaa aatacatcga atcggtaaaa cagacatcgt
     5161 cgaaactaaa gttcgctata ggttgcggat gcacatgaat aattttcgaa acagtatcaa
     5221 cacagccttg attggtagtg gcagttaatg taactacata ttctccaaag ctgttgtata
     5281 aatgtacagg attaatttcg ttcgaaaaat taccatcacc aaaagcccaa aacgacatgg
     5341 cactaccgta ttctatatat gtggtgttgt tgaaactgag cggtgttccg tcgcattgat
     5401 tttgtgtgat aaaattggga atggaccgcg gaaaaatttc gatgttatgc tttaccgaat
     5461 cgctacaacc cgattcaatt tgtgtaacaa ctaggcttac cgcatagttg ccggctgcag
     5521 gatacacatg gtttgggttt tcgatatccg acatacttcc gtctccaaaa ttccattcat
     5581 atgccagtgc tcctccgttg ggcgcaaacg aattgttaac acaaaacagg gtgtcggcat
     5641 ggcacttgtt ggcagcataa aaactaacta ccggcgaggg ataaacagga acaacagcac
     5701 ttattgaatc gatgcacgaa tgatttgata ctgcaaccaa ttttactgta aacatgccgt
     5761 aagaaggata aatataaaca ggattagttg agctgtttcc gtcgccattg ccaaaatccc
     5821 aactgtagct tacaatggag cccgccgaaa tgctgctgct gtttacaaag cttgtcggat
     5881 gcgaataaca aacagtatca taagtgaacc cgacatcagg tattggatgc actacaatgg
     5941 tacaaatttc ggaataggca gcggcacatg ctcccgattg tatcaccgtt cgatacattg
     6001 tggtttggtt taaattgctg aaatttagtg tgtgagtggc attggctgtg tcgttccaag
     6061 tagtgccata atcgaatgaa tactgccatt tcataattgt gcctgtataa tcggaaagaa
     6121 ccagagtgcc actgttgttg ccagcacata cttcctgatg acccgacaca attccgccat
     6181 gcgataccgg tgatacgtac acctcaactg tattggtata tatcgactgg caaacaccac
     6241 ttttcaccaa agccctgaaa tggtgtgtct gcaataaatt ggtatattca agcgtatcgt
     6301 tttgataatc gatgctcgac cagggagaaa aacctgtttg cgacgattcc catccgacaa
     6361 ggtctccagt gtggcctgat acaatgagtt gtccgttgtt actcagggca caaacatcgt
     6421 tgttcccctc gagtgttcca gtttgggtgg gcggatatat cgtaacaact gctgtgtttg
     6481 atatggcttc gctgcatgca ccactttgta ccacagtgcg ataccaattg gtgctcgata
     6541 ggttgttgaa cgaatagctc gggttggtgt ttgcaatggt attccacgaa acaccttcgt
     6601 tgttggagta ttcccaacgt gtaatattgc cgttgtagtt gcttaagttg agtgtgccgg
     6661 agtttccact accacacaat tccatgttgc caactaagtt gcctgcttga gtgaggctgc
     6721 ttacatgaac tacggcaacc gtgcttgtgt cttgatcggt cgctccaaat ttcacaatag
     6781 ccctgaaata ggtagtctgc tgcaaattgt tgaagggtaa gggtttgctt tgatacgata
     6841 tagtaatcca tgggtcgctg cctgatattg agtattccca gtataacacg tcgcctaccg
     6901 acgacattag tgcgatggtg ccggtgttga catcggcaca cactgtgcca ggtgatgcta
     6961 ccgcagtacc accatcagac acttgtgaat atgtggccgt tgaccacagc agaaaactca
     7021 acatcacgat tactgactta aatgaaaaat cattcataag ctacattatt acaattttat
     7081 aggttttcat tttgttgagt acattcattc gtagaatata gattcccgga tccaaatgat
     7141 taaagttaaa cgacttattt acatacgatg catggtcaat ctgctcttga taaatcattt
     7201 ttccatgtaa atcgaataag gacagcgaaa ttactgagtg ttgattgagc tgtaactcgt
     7261 aagtaaaact accatcattg gggttgggat agagtttttc ggaaagaatt tcgaaaaagt
     7321 gttcggtgtt atcgttttca acttctttgg gaatataaat tttgcttgta ccgcttattt
     7381 gtattggctt tgaaatggta tcggaacaaa tattgttgct taccatcagt gtaaccagat
     7441 agttgccttc tacatagaat acatgctgag gaattgcttc ggtgcttgta acgccgtctt
     7501 taaaatccca taaataatgg gttggttcag gatatgaaac atcaacaaaa cgtattgtgt
     7561 cgccccgcat ggcctccgat gctgccagaa aatatgcggt aagcgaaagc tcgctgaatt
     7621 gaactatcac tgtatcggaa gcagtgcaac caaattcgtt ggtaacatca acccaatatt
     7681 tacctgaatc ggccacgatt actgcattct caaaagagct aaaaccgaaa tcagaacccc
     7741 aagcatagta ttgtccggca tcaccggcac ttaacaaagc catttgattg gcacagagat
     7801 acaaatcagc acctaaatgt actattgggt tgggatgaaa aactactgca acagaatctt
     7861 cggagctaca accaaattcg ttggtaactt gcacagtata attccctgtt tctacaacct
     7921 gtatcgtggc gcttgtttga agtgtgttcc agagaaagct gcaattggga tttcc
//
LOCUS       DAOOQP010000130         7959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11226_length_7959_cov_5.880946, whole genome shotgun sequence.
ACCESSION   DAOOQP010000130 DAOOQP010000000
VERSION     DAOOQP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11226_length_7959_cov_5.880946"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLK75_11215"
     CDS             <1..951
                     /locus_tag="PLK75_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOY39857.1"
                     /translation="KKNIIKIPILIIAGILIGMIISNLFIFSSKTEEKTRDIFNFQDS
                     LFQYSVISPEIPDSVFFAGERVPLEYFDVYESLDMEMLINTYRHSSTILYIKRAERYF
                     PIIEEILRQNGIPDDFKYLCVAESGLSNAISPSQAVGFWQFLKATAQEYKMEVNNNVD
                     ERYNVIASTQAACNYLKAAYRIFNSWSLAAVSYNMGMGGLSQRIKHQKVNTYWDLYLH
                     EEPARYVYRIISLKLIMENPERYGFYMKDTDKYSPISTREVIVDTSINRLQEFAFSQG
                     INYKMLKVFNPWLRDTLLVNRSGKKYTIVIPENGARIIAR"
     gene            1000..3825
                     /gene="uvrA"
                     /locus_tag="PLK75_11220"
     CDS             1000..3825
                     /gene="uvrA"
                     /locus_tag="PLK75_11220"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOY39858.1"
                     /translation="MTETESIEIFGAKVHNLNNIDLQIPRNKLVVITGLSGSGKSSLA
                     FDTIYAEGQRRYLETFSAYARQFIGNLERPEVDKITGLSPVISIEQKTTVKNPRSTVG
                     TITEIYDYLRLLYARASIAYSYNTGEEMVRYTDEQIIQNIIHSYSGKPVYVLAPLVDG
                     RKGHYRELFESLRKKGFLNARIDGKVQEISHSLKLERYKSHFIELVIDKLIPDNKDHK
                     RLKSSIETAMKFGKGIIMILEKDGTDARYFSKFLMCPTTGVSYNEPAPHNFSFNSPLG
                     ACPNCNGLGWLPDIDINKIIPDENKSISDGAIVPLGKYKNMFSFWIIEAICDKFKCSI
                     QTPVSELPDAVMNIILYGCEEPIHLKNTPLGANPGYVVHFEGIINQLRNKQSEDDSNN
                     KNNSDSEQYNTYKVCPVCNGLRLKKESLHFRISGRNIAQLAQMDVSDLYNWFLELPAQ
                     LNARQKSIASELIKEISIRLDFLLKVGLDYLSLDRPTKSLSGGETQRIRLATQIGSRL
                     INVLYILDEPSIGLHHRDNLKLIDSLKELRNRGNSIIVVEHDKDMIMSADYIVDIGPA
                     AGKNGGDIVFAGEPHEMLDSGSITAAYINGTKKIEIPSKRRKGNGKSLQIIGATGNNL
                     KNIDISLPLGMIICVTGVSGSGKSSIIHETLFPVLSGHFYRSKTQALPCTEIKGIENI
                     DKVIEVDQMPIGKTPRSNPSTYTGLFSDIRKLYESLPESKIRGYKLGRFSFNVKGGRC
                     ETCQGAGVKIIEMNFLPEVHVPCEVCNGKRYNRETLEVKFKGKSIADILDMTIDRAAD
                     FFENIPFIQHKLKMLQEVGLGYISLGQSSTTLSGGESQRIKLATELMKKDTGKTLYIL
                     DEPTTGLHFEDIRVLMHVLQKLADKGNTLIIIEHNMDVIKCADHIIDMGPEGGNKGGQ
                     IVAEGSPEEIALHKNSYTGYFLKSELL"
     gene            4013..4240
                     /locus_tag="PLK75_11225"
     CDS             4013..4240
                     /locus_tag="PLK75_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39859.1"
                     /translation="MGKDFDPFMDDMKEFEDDDFDSIEEFEEEDLEGLNDDTEFGEFD
                     DFDEDEGEDFDFDEFDEDDDEDLDNFDEDDN"
     gene            4377..5669
                     /gene="mtaB"
                     /locus_tag="PLK75_11230"
     CDS             4377..5669
                     /gene="mtaB"
                     /locus_tag="PLK75_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOY39860.1"
                     /translation="MTIAKTIAFCTLGCKLNFAETSSISQEFAALGFKIVDFKNYADV
                     YVINSCTVTATANKKSRNAIQRAYHLNPAAVIAVTGCYAQLKADEIKRIHKDIIVVGN
                     FEKDNISGLVQRKLNNEISEPLQICHPERFIPAYSIGNRTRSFLKIQDGCDYKCSYCT
                     IPLARGKSRNQSIENTAKHASILIKSNINELVLTGVNIGDFGKSTNERFIDLLKRLDS
                     TEGMVRIRIGSIEPDLLSDEIIAFMAASKHIMPHFHIPLQSGSNTMLRLMRRRYTTEI
                     FADRIRTIKKYLPLSFVGCDVIVGMRGESEKYFEDSYTFIESQDISFIHVFSYSEREH
                     TDALAISGPVGFPEIQRRSNRLHQLSALKHKQFYQKNIGQHQHILWESSKKNMQMFGF
                     TENYIKAEMPYEACNINTITHVELIGLSESGNMKIRTL"
     gene            5666..6472
                     /locus_tag="PLK75_11235"
     CDS             5666..6472
                     /locus_tag="PLK75_11235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOY39861.1"
                     /translation="MNLNLSSICTAVESIAGEAASLIRNERSKLQSSEIEIKGLNDLV
                     TYIDKSSEQFIVESLKDIVPNAGYITEENSIKPEKADYQWIIDPLDGTTNFVHGLQPY
                     AVSIALMYRSTTLLGVVYEITGSECYSAIKSKGAFMNSKRIKVSSTSNIKNSLYVTGF
                     PVSNFSRNKEYFRLMDYLIQNSHGVRRLGSAAADLAFVACGRLDAFYEYDLKPWDVAG
                     GAIIVEEAGGRVGDFSGLPNHIFGKEIIASNSLIFSDLQNIIADFMQHKK"
     gene            6505..7950
                     /locus_tag="PLK75_11240"
     CDS             6505..7950
                     /locus_tag="PLK75_11240"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOY39862.1"
                     /translation="MKNLLLCIIVSMLSSSLFASNLEAYFMYSSFNSPEGPYIDACIS
                     TIGNSAVFVKNENGKYNAEIEVTLVFYKNGEVYTYRKYNLKSPEVSDSTGAKPNFIDV
                     QRVSLVNGIYNLELIIKDIHAESDQYLYSDIINIEYPEKALKFSNIQYIESYKASETP
                     TVLTKHGYDLVPYISDFYPSNIDKLIFYVEMYNVKQITTDPVVLTYYIEKFENSEKID
                     MYNRFKKMESQDYNVFIGELMITELYSGNYNLVVEVRNRNNEIIGSTKAFFQRSKRSP
                     TDANPANQNSYNLQFAGISENLDTVRHYILSLRPIANNNECNFIDYQVKTADMQTMQN
                     FFSSFWIKRSPADPTGEWGIYKAQVDYVNKWYSTPIKKGFETDRGRVYLKYGTPNDIY
                     VSKHEPTAYPYEIWHYYKIGDETNRKFVFYNPSIAGEEYELLHADVTGELRNPSWERQ
                     LNKRNNTLYNPDQMNSDDQWGGRAKDEYNKR"
BASE COUNT         2673 a         1459 c         1624 g         2203 t
ORIGIN      
        1 aaaaaaaaca ttattaaaat tcccatcctg attattgcag gtatactgat aggcatgatt
       61 atcagcaacc tgttcatctt cagtagcaaa accgaagaga aaacccgtga tatttttaat
      121 ttccaggata gtttatttca atattctgta atatcacccg aaatacccga ttcggttttc
      181 tttgcaggtg aaagagtccc ccttgagtat tttgatgttt atgaaagcct tgatatggag
      241 atgctcataa acacctaccg gcattcctcc accattctgt atattaaaag ggctgagcga
      301 tacttcccaa taattgaaga aatactcagg caaaacggca ttcccgacga cttcaaatat
      361 ttatgtgttg cagaaagcgg attgagtaac gcaatcagtc catctcaagc ggtaggtttc
      421 tggcaattcc tgaaagcaac ggctcaggaa tataaaatgg aggtaaacaa caatgttgat
      481 gaaaggtata atgtaattgc ttcaactcag gctgcctgca attatttaaa agccgcttac
      541 cgcattttca acagttggtc actggccgcg gtttcctaca acatgggtat gggcgggctg
      601 tcgcagcgca taaagcatca gaaagtaaac acatactggg atttatatct acatgaagag
      661 cctgcccgct atgtatatcg tattatcagt ttaaaactta tcatggaaaa tcctgagaga
      721 tacgggtttt atatgaaaga tacggacaaa tactctccta taagtactcg cgaggtaatt
      781 gttgacacat cgataaatcg attgcaggaa tttgcttttt cgcaaggaat taattataaa
      841 atgctgaaag tttttaatcc atggcttcgc gacacccttt tggtaaatcg gtccggaaaa
      901 aaatacacaa tagtgattcc ggaaaatggc gcacgaataa tagccaggta aattattaaa
      961 ggggtaacag tttttaaatc aaacgattgc aagaagggta tgacagaaac tgaaagcata
     1021 gaaatctttg gggcgaaagt ccataatctt aataacatcg acttacaaat acccagaaat
     1081 aaactggtgg ttataaccgg tttaagcggt agtgggaaat cgtcgcttgc atttgatact
     1141 atatatgctg aagggcaacg caggtacctg gagacatttt cggcatacgc ccgccaattt
     1201 attggtaatc tggaaagacc cgaagtagat aaaatcaccg gattaagccc tgtaatatca
     1261 attgagcaaa agacaaccgt taaaaaccct cggtcaactg ttggaacaat aactgagata
     1321 tacgattatc tcaggctatt gtatgcgcgt gcatcaatag catattccta caataccggt
     1381 gaagaaatgg ttcgctatac cgatgaacaa attatacaga atataattca ctcctattca
     1441 ggtaaaccgg tatatgttct ggcaccgctt gttgatggaa gaaaagggca ttatcgtgaa
     1501 ttatttgaat cgcttcgcaa aaaaggcttc ctaaatgccc gtattgatgg aaaagttcag
     1561 gagataagtc attcattaaa acttgagcga tataaatcgc atttcataga attggttatc
     1621 gacaagctga taccggacaa caaagaccat aagcgattga aatcatccat agaaaccgca
     1681 atgaaatttg gcaaaggtat tatcatgata cttgaaaaag atggaaccga tgcccggtat
     1741 ttcagcaagt ttttgatgtg ccctacaacc ggagtttctt acaatgaacc tgcaccgcac
     1801 aacttctcat tcaattcccc attaggggca tgtccgaatt gcaacggttt ggggtggttg
     1861 cctgatatcg acattaataa aataattccg gatgaaaata aaagcatcag tgacggtgct
     1921 attgttccat tggggaaata taaaaatatg ttttcatttt ggattatcga agctatttgc
     1981 gataaattca aatgttcaat acaaacaccg gtttcggagc tgcccgatgc cgtaatgaat
     2041 attattctgt acggctgcga agaacccatt cacctgaaaa acacacctct tggtgcaaat
     2101 ccagggtatg tagttcactt tgagggaata ataaaccagc taaggaacaa gcaatcagaa
     2161 gatgatagta acaataaaaa caattccgat tctgaacaat acaacacgta taaggtgtgc
     2221 ccggtttgta atggattgcg gttaaaaaaa gaatcattac acttcaggat ttcaggtaga
     2281 aacattgcac aacttgctca gatggatgtt tctgacttgt acaactggtt tcttgaatta
     2341 ccggcacagc taaatgcacg gcaaaaaagc atcgcttcag aacttattaa ggaaataagc
     2401 atccgcctgg attttttact aaaagttgga cttgattacc tttcgcttga cagacctaca
     2461 aagtcgttat cggggggtga aacccaacga atccggcttg caacccaaat tggctccaga
     2521 cttataaatg tgctgtacat tcttgatgaa cccagcattg gcctgcatca tcgcgataac
     2581 ctgaaactaa tcgattcact taaagaactg cgaaacagag ggaattcaat aatagttgta
     2641 gaacacgaca aggacatgat tatgtcggca gattatattg ttgatatcgg gcctgctgct
     2701 ggtaaaaacg gaggagatat agtctttgcc ggagagccac atgaaatgtt agattcaggg
     2761 agtattaccg ctgcctatat taacggaaca aaaaaaattg aaatcccatc gaaacgtcgt
     2821 aagggcaacg ggaagtcact tcagataatc ggtgctacgg gtaataattt aaaaaacatt
     2881 gacattagcc ttccgcttgg aatgataatt tgtgttaccg gtgtttccgg aagcggaaaa
     2941 tcgagtatca ttcacgaaac cttgtttccc gttttaagtg ggcattttta tcgctccaaa
     3001 acacaagccc tgccttgcac cgaaatcaaa ggcatcgaga atatcgataa ggtaatagaa
     3061 gtcgatcaga tgccgattgg gaaaacaccc cgctcgaacc cttcgaccta tacaggattg
     3121 ttttcagata tccggaaact ttatgaaagt ttgcctgaat cgaaaattcg gggctacaag
     3181 ctaggccgtt tttcattcaa tgtaaaaggg ggaagatgcg aaacgtgcca gggtgccggt
     3241 gtaaaaatta ttgaaatgaa ctttcttcct gaagtacatg ttccctgcga agtatgtaac
     3301 gggaaacgtt ataacagaga aacccttgaa gtaaagttta aggggaaatc gattgcggat
     3361 attctggata tgaccataga cagggctgct gattttttcg aaaatattcc tttcatacag
     3421 cataaactca agatgcttca ggaagtcgga ttaggttata tctcactcgg gcagtcatcg
     3481 acaactttat cgggtggtga atcacaaagg ataaagctcg caactgagct tatgaaaaag
     3541 gataccggga aaaccctgta cattcttgat gagccaacaa caggtcttca tttcgaagat
     3601 atcagggtgc tgatgcatgt gctacaaaaa cttgccgata aagggaacac actgattatt
     3661 attgagcata acatggacgt tattaaatgc gcagaccata ttattgacat gggaccggaa
     3721 gggggtaaca aaggtggtca aatagttgca gaaggctcac ccgaagaaat agctctccat
     3781 aaaaacagct atacagggta tttcctgaag tcggagttgc tgtagaactc aattcaaaca
     3841 tgggcaactt ggctttacca ttgattttca gaatattaca cacaagacat ttttaaaaca
     3901 aaaaatattt tggcaaaaga atttcttcag cgaattttat tgtgttatta ttttttttaa
     3961 taagtttacc cacagaatta actcaagttt ttgaacaaaa aaaagtatag ctatgggtaa
     4021 agattttgat cccttcatgg acgatatgaa agaattcgaa gatgatgatt tcgacagtat
     4081 cgaagaattc gaagaggaag atttggaagg actaaacgac gatactgaat ttggtgaatt
     4141 cgacgacttt gatgaagacg aaggtgagga ttttgatttt gatgagtttg atgaggacga
     4201 tgatgaagac ctcgataact ttgacgaaga cgacaattaa tccacattag aaaaatatta
     4261 tttcgtgcgg gaggcgtcgg tgcccgtata ttacaactat attttaagaa cattgtactt
     4321 caaagggagt atttaaacac aagtgcattt taaagctttt tttcacctgt ataatcatga
     4381 caattgcaaa aacaatagca ttttgtacgt tgggatgtaa gctcaatttt gccgaaacct
     4441 cctcaatatc acaggaattc gcagcacttg gttttaaaat cgtcgatttt aaaaattatg
     4501 ctgatgtata tgtaataaat tcgtgcacag ttaccgccac agcaaacaaa aaaagcagaa
     4561 atgccattca gcgggcttac catttgaacc cagcagcagt tatagcagtt acaggctgtt
     4621 atgcccaatt aaaagctgat gaaataaaaa ggatacacaa ggatatcatt gttgttggaa
     4681 attttgagaa agacaacatt tcgggtttgg tacaacgaaa gttaaataat gaaatatctg
     4741 aaccattgca aatctgtcac cccgaacggt ttattcccgc ctacagtatt gggaacagaa
     4801 cacgtagttt tctgaaaata caggatgggt gcgactataa atgttcatac tgcaccattc
     4861 ctttagctag gggaaaaagt cgaaaccagt cgattgagaa tacggcaaag cacgcaagca
     4921 ttctgataaa aagcaatatc aacgaacttg ttctcaccgg tgtgaatatc ggagattttg
     4981 gaaaatcaac gaatgaacga tttatcgatt tacttaaacg gctagacagt acagaaggga
     5041 tggtccggat tcgaatcggg agcattgaac ccgacctgct tagcgatgag attattgctt
     5101 ttatggcagc atcgaaacat attatgccgc attttcatat tccgctgcaa tcggggagca
     5161 atacaatgct gaggttgatg cgacggcgat acactaccga aatatttgcc gatagaatca
     5221 gaacaataaa gaaatacctt ccgctttcgt ttgttggttg tgatgttatt gttggaatga
     5281 ggggagaatc ggaaaagtat tttgaagact cctacacttt tatcgaaagc caggatattt
     5341 catttataca tgttttcagc tattctgaaa gagaacacac tgatgcactt gctattagcg
     5401 gacctgtggg ctttcccgaa attcaacgaa ggagcaatcg tttacatcaa ctatcggcat
     5461 tgaaacacaa acaattctac cagaaaaata tcgggcagca ccagcatata ctttgggaat
     5521 ccagcaaaaa aaacatgcaa atgtttggtt tcaccgaaaa ctacataaaa gcagaaatgc
     5581 cttacgaggc ttgtaatatt aacacaatca cacatgtaga acttattgga ttatcggaat
     5641 ccggtaacat gaaaatcaga actctatgaa tttgaattta tcgtcaattt gcactgcagt
     5701 tgaatcaata gcaggcgaag cagcaagtct tatccgaaat gaaaggtcaa agcttcagtc
     5761 ttcggaaatt gagattaagg ggttgaacga ccttgtaact tatattgaca aatcatcgga
     5821 gcaatttatt gtggaatcgt tgaaggacat tgtcccaaat gcaggttaca taacagagga
     5881 gaattccatt aagcctgaaa aagccgacta tcagtggata attgaccccc ttgacggtac
     5941 aacgaatttc gttcatggct tacaacctta tgctgtaagt attgcattaa tgtatcgcag
     6001 caccacatta cttggagttg tgtatgaaat aaccggctct gaatgttaca gcgcaataaa
     6061 atcaaaagga gctttcatga attcgaaaag aattaaagta tcctctactt caaatatcaa
     6121 aaacagccta tatgtcaccg gctttcccgt aagcaatttt agcagaaata aagaatactt
     6181 caggctaatg gattatctta tccaaaatag ccacggtgtg aggagattgg gctcagcagc
     6241 agccgatctt gcctttgttg cttgtggaag attagatgct ttttatgaat atgaccttaa
     6301 accttgggat gtggcaggag gagccataat tgttgaagaa gccggaggaa gagttggcga
     6361 tttttcgggt ttacccaatc acattttcgg caaagaaata atagcaagta atagtttgat
     6421 atttagtgat ttgcaaaata ttattgccga ctttatgcaa cataaaaaat aatggctatc
     6481 ttgtgtagtt aaaattcatc aactatgaaa aatttgctct tgtgtattat cgttagcatg
     6541 cttagttctt cgctatttgc aagtaatctg gaagcatatt tcatgtattc aagcttcaat
     6601 tcaccggaag ggccgtatat tgatgcttgt ataagcacta taggtaattc agctgttttt
     6661 gtcaagaatg aaaatggtaa atacaatgct gaaatcgaag ttactctggt gttctacaaa
     6721 aacggggagg tttacacata tcgtaaatac aatctcaaaa gtcctgaagt ttcggattct
     6781 acaggagcaa aacctaattt tattgatgtg cagcgagttt ctcttgtaaa cggtatttat
     6841 aatctcgaac tcattattaa ggatatacat gcagaaagcg atcaatatct gtattccgat
     6901 attatcaata ttgagtaccc tgagaaagca ttaaaattca gcaatattca atatattgaa
     6961 tcatataaag catcggaaac acctactgtg ctcacaaaac acggatatga tctggttcca
     7021 tatatttctg acttttaccc ttcgaatatt gataagttga ttttttatgt agaaatgtac
     7081 aacgtaaagc aaataaccac tgaccctgtt gtactaacct actatatcga aaaattcgaa
     7141 aacagcgaaa aaattgatat gtataacagg tttaaaaaaa tggaatcgca ggactacaat
     7201 gttttcattg gagaactcat gattactgaa ttatactcgg gtaattataa tcttgtggtt
     7261 gaggtcagga atcgcaacaa cgaaattatc ggatcgacaa aagctttttt tcagcggagt
     7321 aaaaggtctc caactgatgc aaatcctgcc aatcagaaca gctataactt acaattcgca
     7381 gggatttcgg aaaacctcga tactgttaga cactatatcc taagtctgcg ccccatcgca
     7441 aacaacaatg aatgtaattt tattgattat caggtaaaaa ctgctgatat gcaaacgatg
     7501 cagaatttct tctcgagctt ctggattaaa cgcagccctg ccgacccaac cggtgaatgg
     7561 ggaatataca aagctcaggt tgactatgtg aataagtggt attccactcc aattaaaaaa
     7621 ggttttgaaa ccgatcgtgg cagagtatat ctgaaatatg gaacacctaa cgatatttac
     7681 gtcagcaaac acgaacccac agcatatcca tacgaaattt ggcactatta taagatagga
     7741 gatgaaacaa accgtaagtt tgttttttac aatccttcga ttgcaggaga agaatatgaa
     7801 ttacttcatg ctgatgtaac cggtgaatta cgtaatccaa gctgggagcg gcagttaaat
     7861 aaaagaaaca atacactgta taatcccgat cagatgaata gcgatgacca atggggaggc
     7921 cgtgccaaag atgaatacaa caagcgataa gagtctgga
//
LOCUS       DAOOQP010000131         7881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11371_length_7881_cov_5.919882, whole genome shotgun sequence.
ACCESSION   DAOOQP010000131 DAOOQP010000000
VERSION     DAOOQP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_7881_cov_5.919882"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2820)
                     /locus_tag="PLK75_11245"
     CDS             complement(<1..2820)
                     /locus_tag="PLK75_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39863.1"
                     /translation="MKFIIKAILAGSLILSSTALFSQNECSNAVNLPFESYSTCGNLA
                     MQSINLATATHSSTTPDPGCASFGSSTKDFWYTITVPAGINTLAFHMFNSNVTPFLNS
                     SQPGMAVYRGTSCTSLTLLDCFESSGGFMENGEIRFEQISGLVPGETLYIRVWDENNV
                     SQQIFLIVSELLSLPEDDCSTPVPLGTGGCNILSTGGDITAPEDCGWNTTDNSIFYYF
                     TVYPDDPQPYTITAENGECWANSGGENPEIQFAVYSWNGSNCNGIGGSGSSYQGCANG
                     TGVVTFSENLPPGNYILAFDGYSMMSGNSLCIFGFEAPFITPELTVTLNTTNAICGSS
                     GSASITVVESCTGNPVISWSNSASGYTVQNLAPGNYSVTVADGVECGDTVINFTITDS
                     GSLSVDATVSGESCEGDMSATATVTGADPGQCNFAWNTTPPQYTQTVTDLSAGTYTVT
                     VSLGSCTATDQVLVTVYDNITVNNLTTDCNSTNTEFTVSFTVTGSSGGPAPFNANWGS
                     GNQSFTGSFSQNFTSPGTYNITVTDNNNCDEFVLTGNIDCGCQTYAGTMSSLQPIHLC
                     QEECTDAVSHNGDQALDSDDTFEFIIHTGGFPATILGRSNDPVFCLNDIQGGGSFETV
                     YYISSVAGNNSGGHVSQSDQCYSQSVGTPVIWHQNPIAYINGTELETCGNIIHLSAMA
                     PPSGMIAYWSSESQFVPIGGSMLSSPEINVLVNTYGDITFYWNVQNQQCTGRDSILVH
                     FYQTPSAYAGEDMTVCGNTVDLEAVLSLETSNGLWSGNGATFSQGTNPESNVIVSNFG
                     TYVFTWSEFNGTCFDQDNVTINFIQTPTPTVSMNHDTICGVSYNLHVYNVTGEGIWTA
                     FDDGVPITPTYVPPSTPYSPDAVVVVPAYSGLYNTIEFCWTEINDVNGIQCTAQACIE
                     VTFSREPSASVGPDSWEQVCGNVFTF"
     gene            complement(3029..3103)
                     /locus_tag="PLK75_11250"
     tRNA            complement(3029..3103)
                     /locus_tag="PLK75_11250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3066..3068),aa:Pro,seq:tgg)
     gene            3219..4670
                     /locus_tag="PLK75_11255"
     CDS             3219..4670
                     /locus_tag="PLK75_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOY39864.1"
                     /translation="MIQIRKILLVLGILLAVESIFLALSGIIAVIYKGNDIFSLFLVT
                     AISLILGISLFLFSRKTKREISKKESYLIVALSWIVLSAVGALPFVFHGCIPSYTDAF
                     FETVSGFTTTGATILNDIESVPHGLLFWRSITQWMGGMGIIVLSIAVFPLIKVGGFQL
                     FVAEVPGVTYDKLHPRIKGTAKRLWLIYIGLTAAEIILLTLGGMTLYDAVNHSFTTMA
                     TGGYSTKQASIAFYNSPFIQYVIIFFMFLAGVNFSLIFYAFKGQFRKLIRNNEFRFYT
                     LITIVSAVFVALGLMFYMNLPFEKAVRDALFQVVSIITTTGFITADYMLWVPPYLWVI
                     IFLLMFVGGSSGSTGGGIKAVRIHLILRNSYLELKRLIHPNAVIPVRYNGQSVSSGII
                     TNVMAFVIIYFIIFGAGTIILAINGLDLDSAMGASATCLGNIGPGINQLGPAYTFSFL
                     NDISKWTLSALMLLGRLELFTILIIFTPVFWKK"
     gene            complement(4674..5240)
                     /locus_tag="PLK75_11260"
     CDS             complement(4674..5240)
                     /locus_tag="PLK75_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39865.1"
                     /translation="MKTLLIFGISILLLLYYNISPAQNLKWVEAELVYNNGGRQNGLI
                     KARKNSCFAFMKCKTDTESKAFVVHPDEVNEVITDAFHFFSIHFNESDHFIKSSSFGI
                     LHGGNEVKLLETTFFYKSCSCNSRGSYRRGYFLWSDNDYMYVKLHAGNIVSNPGELNI
                     FLSKHNFEVFSNTSSTSLNDLLNHLNRN"
     gene            complement(5237..5731)
                     /gene="ribH"
                     /locus_tag="PLK75_11265"
     CDS             complement(5237..5731)
                     /gene="ribH"
                     /locus_tag="PLK75_11265"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOY39866.1"
                     /translation="MSSALNKSPLSGTEVLPKGIHAQIGIVVSKWNSEITDKLLAGAI
                     QTLTEKGISEDCIKVEFVPGSFEIPLAAQWLIETYGCDSVICLGCVIQGETRHFDFIC
                     NSVANGIMDLNLKFGIPVIFGVLTTNNMHQAIDRSGGKYGNKGIEAASTAISMISLKF
                     KCSL"
     gene            complement(5788..6486)
                     /locus_tag="PLK75_11270"
     CDS             complement(5788..6486)
                     /locus_tag="PLK75_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39867.1"
                     /translation="MSKEKKIQKQAESNIGTVQNALSRTEQFIENNQKIIIYVIVGLV
                     VVVGGYLAYRSFIQQPREKRASAEMYTAQYYFELDSFKLALQGDGKNAGFLNIIDDYG
                     STKAGNVANYYAGVCYMRLGEYNTAIGYLEDFDSDDKILKPISIGLIGDANAELGKLD
                     LAIEKYLEAADQASNDFVSPIYLMRAGIAYESLNKWNEALEIYNRIDKEYYGTSEQRN
                     IEKYITRAKLKAGK"
     gene            6654..7739
                     /gene="recF"
                     /locus_tag="PLK75_11275"
     CDS             6654..7739
                     /gene="recF"
                     /locus_tag="PLK75_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOY39868.1"
                     /translation="MYLKTLTLVNFRNFRSFGYDASQRINCFVGNNGVGKTNLLEAIH
                     YLSFSKSFINYSDRLNITHGEEMFLIQGDYERNGSSEKIYCAFKSGQKKVLRHNGKEY
                     EKMSDHIGFIPLVYALPSDSTMIFAGSEQRRRFIDQVISQFDHNYLNELIAYNHGIEQ
                     RNKMLKDAARESKFDNLSFEVWDDFLSEKSAYIYKVRNDFINGFLSSFQKYFELISGG
                     NETVELIYESQLASENPLTLLKKSFHRDSVLQYTTTGVHRDELIFNIEKHALRSTGSQ
                     GQQKSFVAALKFAQFDFIKNKTGLAPILLLDDIFDKLDHERVGNIMNIVSGEQFGQIF
                     ITDTDEKRIRNMLSHIDIESNILNIYE"
BASE COUNT         2276 a         1534 c         1624 g         2447 t
ORIGIN      
        1 gaaagtgaat acattgccgc aaacctgttc ccagctgtca ggtccaacac tggctgatgg
       61 ctctcttgag aaagttactt caatgcaagc ctgagcagtg cactgaattc cgtttacgtc
      121 attaatttct gtccagcaga attcaatggt gttgtaaagg ccggaataag caggcacaac
      181 cacaactgca tcagggctat aaggcgttga aggaggaaca tatgttggag taatcggaac
      241 accgtcatca aatgcagtcc aaataccttc accggtaaca ttatatacat gtaaattgta
      301 gctcactcca caaatggtat cgtgattcat cgagacagta ggagttgggg tttgaatgaa
      361 atttatggta acattgtcct gatcaaagca tgtaccgttg aattccgacc aggtgaaaac
      421 atacgttccg aagtttgaaa caataacatt gctttcaggg ttagttccct gtgaaaatgt
      481 agcgccatta ccgctccaaa gtccgtttga tgtttcaagg ctgagcactg cttccagatc
      541 aactgtattt ccacaaacgg tcatatcttc cccggcatag gcacttgggg tttggtagaa
      601 atgaacgaga atgctgtcgc ggcccgtgca ttgctggttt tgaacattcc agtaaaaagt
      661 gatatctccg taagtattaa ccaagacatt aatttcggga cttgataaca tgcttccacc
      721 aataggtaca aattgtgatt ccgacgacca gtatgcaatc attcccgaag gtggtgccat
      781 agcactgaga tgaattatat taccgcaggt ttccaattcg gtcccattga tgtaggcaat
      841 ggggttttgg tgccatatta cgggggttcc cacggattga ctatagcatt gatcactttg
      901 gctcacatgt ccgccagaat tatttcctgc aactgatgaa atatagtata ctgtttcaaa
      961 actaccgcct ccctgaatgt cgttgaggca aaaaaccgga tcgttgcttc tgccgagaat
     1021 ggttgcagga aagcctcctg tgtgaatgat aaattcaaag gtatcgtcgc tgtccagtgc
     1081 ctgatcacca ttatgactga cagcatcagt gcattcttcc tgacacaggt gaattggctg
     1141 caggctgctc atggttcctg catatgtctg acacccgcag tcgatatttc cggtaagcac
     1201 aaattcatcg cagttgttat tgtcggtaac tgtgatattg tatgtcccgg gagatgtaaa
     1261 gttctgtgag aacgaccctg taaagctctg gttcccgctc ccccagtttg cattgaaagg
     1321 cgccggcccc cctgaagagc cggtaaccgt aaagctgact gtaaactccg tattggtgga
     1381 gttacaatct gtggtaagat tatttacagt gatgttatca tatacggtaa ccaatacctg
     1441 atcagtagct gtacatgatc cgagtgaaac agtaacagta taggttccag ccgatagatc
     1501 tgtaacagtt tgggtatatt gtggaggtgt ggtgttccac gcgaagttac actgaccggg
     1561 gtctgcccct gtcacagttg ctgttgcact catatcgcct tcacaggatt ctcctgagac
     1621 ggttgcatct actgagagtg atccggaatc ggtaatcgtg aagttaatta ctgtatcacc
     1681 acattccacc ccatcggcaa ccgttaccga gtaattgccg ggtgcaagat tttgcactgt
     1741 gtagcccgaa gcactgttgc tccacgaaat tactgggttg cctgtgcagg attcaactac
     1801 tgttatagag gccgacccgc ttgatccgca gatagcattc gtcgtattca gtgttactgt
     1861 aagttccggt gtaatgaatg gtgcttcgaa tccgaatatg caaagcgagt ttcctgacat
     1921 catgctatat ccgtcgaagg cgagaatata atttccggga ggcagatttt cggaaaaggt
     1981 aaccacacct gtcccattgg cgcaaccctg atagctgctt ccagaaccac cgatgccgtt
     2041 acagttcgac ccgttccagc tatacacggc aaactgaatt tcggggttct ccccgcctga
     2101 gtttgcccag cactcaccgt tttcggcagt gatggtgtaa ggttgggggt catcggggta
     2161 aacagtaaaa taatagaata tggagttatc tgttgtgttc cagccgcaat cttccggagc
     2221 cgtaatgtcg cctccggtgg aaagaatatt acacccgcct gtgcctaaag gcaccggtgt
     2281 tgagcaatca tcttcgggta agctcagtaa ttcggaaaca attagaaaaa tttgttgcga
     2341 cacattgttc tcatcccaaa ctcttatata cagagtttct cctggaacaa gcccgcttat
     2401 ttgttcaaac ctgatttctc cgttttccat gaagccacct gacgattcga aacaatcaag
     2461 caatgtaagg ctggtacaac tggtgccgcg gtaaactgcc attccaggct gggaactgtt
     2521 aagaaacggt gtaacattgg aattaaacat atgaaatgcc agagtgttaa ttccggcggg
     2581 tacagtaatg gtgtaccaaa aatctttggt agaacttcca aaactggcgc acccgggatc
     2641 tggggttgta gaggaatggg ttgcagtagc aaggtttata gactgcatcg caagattacc
     2701 acatgttgaa taactctcaa aaggcagatt cacagcatta gaacactcat tttgactaaa
     2761 taaagcagtt gacgaaagta ttaatgaacc tgccagtata gccttaataa taaatttcat
     2821 atgatcgttt ttttacattt tgttaacctt acacacaaaa atatttaaat atctcatatt
     2881 taaaatacct taattaaata aaagacaatt aaatagtaat tttagttgtc taaaagctca
     2941 attggaaaaa taaaaaatga attttaaagc aaagaataat atttcaaaaa ttaagcctga
     3001 cgagaaattc atcaggcttt ttttattgtc ggggtagcag gattcgaacc tgcgaccccc
     3061 tggtcccaaa ccaggtgcgc taaccggact gcgctatacc ccgtactgtt ttattttaac
     3121 agcacattca actataaaat tgaggtgcaa aaataataag ttttggttaa tatcaaattc
     3181 atgtgctaat tttttaaatt tgacgaaaat tattaagctt gattcagata cgtaaaatat
     3241 tacttgtgct gggaattcta ctggcggttg agagcatttt tctggctctc tcaggcataa
     3301 tagcagtaat atataaaggt aatgatattt tttcactttt tctggtaacc gcaatatcct
     3361 tgattctcgg aatatcgctc ttcttattca gcagaaaaac aaagcgtgaa atcagcaaaa
     3421 aggagtctta cctgatagtt gctttgagtt ggattgttct ttctgctgtt ggtgccttac
     3481 cctttgtttt tcatggctgt attccttctt atactgatgc gtttttcgaa acagtgtccg
     3541 gctttactac tacaggtgct accatattaa atgatattga atcggttccg catggattat
     3601 tgttttggag aagcatcacg caatggatgg gtggcatggg tattattgta ttgtcgatag
     3661 cggtatttcc acttattaaa gtgggaggat ttcagctttt tgttgctgaa gttccaggtg
     3721 ttacttacga taaacttcat ccccgcatta aaggcactgc caaaaggcta tggctgattt
     3781 acattggatt aacagcagct gaaattatac tgcttacttt aggtgggatg acgttgtatg
     3841 atgccgtaaa ccactcattt actacaatgg ccaccggagg gtattccacc aaacaggcca
     3901 gtattgcatt ttataattcc ccgtttattc aatatgtgat aatatttttt atgtttttgg
     3961 ccggtgtgaa cttttcttta atcttttacg cattcaaagg gcaattccga aagttgattc
     4021 gcaataatga atttagattt tacacgctga ttactattgt ttcggctgtt tttgttgcat
     4081 tgggattgat gttttatatg aatttacctt ttgaaaaagc agtgagggat gcgttgtttc
     4141 aagtggtttc aattattacc accacaggct ttattaccgc cgattatatg ctatgggttc
     4201 ccccatacct ttgggtaata atttttcttt tgatgtttgt tggtgggtcg tcaggatcaa
     4261 caggcggagg aatcaaagcg gtaagaattc atctcatttt acgtaatagt tatctcgagc
     4321 ttaaaaggct tattcatccc aacgcggtaa tacctgtacg gtataacgga cagagtgtaa
     4381 gctcgggaat tataactaat gtaatggcat ttgtaataat atacttcatt atttttggtg
     4441 caggcacaat aattctggca attaacggac ttgaccttga cagcgctatg ggagcatcag
     4501 ccacatgcct gggcaatatc gggccgggta tcaaccaatt gggtcctgct tacacatttt
     4561 cttttttaaa cgatatttca aaatggacat tgtcggcatt aatgctttta gggaggcttg
     4621 aattgtttac aatcctgata attttcacac cagttttctg gaaaaagtaa gtcctaattg
     4681 cggttcaaat gattaagtaa gtcgttcagc gatgttgatg aagtattact gaacacttcg
     4741 aaattgtgtt ttgacaaaaa gatattcaac tctcctgggt tcgaaactat atttccggca
     4801 tgcagtttta catacatata atcgttgtca ctccaaagaa agtacccccg ccggtatgag
     4861 cccctgctgt tgcaactaca gcttttatag aagaatgtgg tttcgagtag ttttacttcg
     4921 ttgccgccat gcaatatccc gaaactgctc gacttaataa agtgatcgct ttcattaaaa
     4981 tgaatactga agaaatgaaa cgcatcggta attacttcgt ttacttcgtc gggatgaaca
     5041 acaaatgctt tgctttcggt atctgtttta catttcataa aagcaaagca actgtttttt
     5101 cttgctttta tgaggccgtt ttgtctgcct ccattgttat ataccaattc agcttcaacc
     5161 cattttaaat tttgcgcagg ggagatgttg taatacaaca gtaacagaat acttattcca
     5221 aaaataagca gtgttttcac aaactgcatt tgaattttag ggatatcatc gaaatggctg
     5281 tgctagcagc ctcaattcct ttgttgccgt atttacctcc ggagcggtcg attgcctgat
     5341 gcatgttgtt tgttgtgaga acaccaaaga tcacaggaat tccaaacttc aggttaaggt
     5401 ccattattcc gttggcaacg ctattgcaaa taaaatcgaa atgacgagtt tcgccttgaa
     5461 taacacatcc caaacaaatt accgaatcgc atccgtaggt ctcaataagc cattgagctg
     5521 ctagaggtat ctcaaaacta cccggaacaa attctacctt aatgcaatcc tctgagatac
     5581 ctttctctgt cagggtttga attgcccctg caagaagttt gtcggtaatt tcgctattcc
     5641 atttcgaaac aacaatccca atctgggcat gaatgccttt gggcaacact tctgtaccag
     5701 acaaaggcga tttatttaat gcactcgaca tactgtagat ttttgtaaaa ataaaaaaaa
     5761 agaggcatag cccctctttt catttaatta tttcccggct tttagttttg ctctggtaat
     5821 atatttttca atattgcgtt gctcactggt gccataatat tctttatcaa tacgattata
     5881 aatttcgagg gcttcgttcc atttattaag gctttcgtat gctatgcctg cacgcataag
     5941 atagattgga gaaacaaagt cgtttgatgc ctgatcagct gcttccagat acttttcgat
     6001 ggcaaggtca agttttccga gttcagcatt tgcatcccca attaaaccaa tggaaattgg
     6061 ttttaatatt ttgtcgtcgg agtcgaagtc ttccagataa ccgatggcgg tattatattc
     6121 tccaagcctc atatagcaaa ctccggcata ataattagcc acattgcctg ctttagtgct
     6181 gccgtaatca tcaataatat ttaggaaacc tgcattttta ccatcacctt gcaaagccag
     6241 tttaaaggag tcaagctcaa aatagtattg tgctgtgtac atttctgctg aagctctttt
     6301 ttcgcggggc tgttgaataa atgaacggta agccagatat ccaccaacca ccactaccaa
     6361 tccaacaata acatatatta ttattttctg attgttttca ataaactgtt cagttctgct
     6421 aagggcattc tgcaccgtgc cgatattgct ttctgcttgt ttttgtatct ttttttcctt
     6481 gctcattttg aataaagttt tttgagaacg caaaggtaac tttttatgaa taattataaa
     6541 attaaattta aaaaagttgt acgaaaattc taaaagccac agggatgtgt tttttggatg
     6601 ctaaaagttg gaggcatttc attttgtttt atttttgaaa caaatatgaa tgaatgtatc
     6661 ttaaaacgct tactcttgta aattttagaa attttcggtc gttcggctac gatgcttcgc
     6721 agcgcataaa ttgctttgtt ggtaataatg gagttggaaa aaccaaccta cttgaagcca
     6781 ttcattattt gtcgttttct aagagcttta taaattattc tgatcgcctc aacataaccc
     6841 atggggagga aatgtttctt attcagggtg attatgagag aaatggaagt tctgaaaaaa
     6901 tatattgcgc atttaaatca gggcagaaaa aagtattgag acacaatggc aaggaatacg
     6961 agaaaatgtc cgatcatatt ggatttattc ccttagtata tgctttgcca tctgactcta
     7021 ccatgatttt cgctgggagc gaacagcggc gccggtttat cgatcaggtg atttctcaat
     7081 tcgatcacaa ttatctgaac gagttgatag cttataacca cgggatagag caacgaaaca
     7141 aaatgcttaa agatgctgca cgcgaaagta aattcgataa tcttagtttc gaagtgtggg
     7201 atgatttcct ttctgagaaa tcagcctata tttataaggt ccgcaacgat tttattaatg
     7261 ggttcttaag tagttttcaa aaatactttg agctaatatc gggtggaaac gaaaccgtgg
     7321 aattaatcta tgagagtcaa ctggcttcag aaaaccctct tacactgctc aaaaaatcgt
     7381 ttcatcggga tagtgtatta cagtatacta ctactggggt tcacagggat gaactgattt
     7441 ttaatattga gaaacacgcg cttaggagta ccggatcaca ggggcagcaa aagtcgtttg
     7501 ttgcagcatt aaaatttgca caattcgatt ttattaaaaa taaaacgggt ttagcaccaa
     7561 ttttgctgtt ggatgatatc tttgataagc tggatcatga gcgggtgggt aatattatga
     7621 atattgtgtc gggagaacag tttggacaaa tattcatcac ggataccgat gagaaaagga
     7681 ttagaaacat gttgtcgcat attgatattg aaagtaacat tttaaatatt tacgaatgaa
     7741 aaaatctttg ttcataataa tattaatata tatttttgcg tcctcgatcg gatacacaca
     7801 aaataaagca aaaagcgatt tgtgtgttta caacgagaaa ctgatggtag gcacctggaa
     7861 aatggatacc atctatttga a
//
LOCUS       DAOOQP010000132         7867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11407_length_7867_cov_4.553891, whole genome shotgun sequence.
ACCESSION   DAOOQP010000132 DAOOQP010000000
VERSION     DAOOQP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11407_length_7867_cov_4.553891"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="PLK75_11280"
     CDS             <1..1907
                     /locus_tag="PLK75_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39869.1"
                     /translation="TETFKFNDEMNIWIPPVYYEKIDGRWWYFVYNMPYESFDECGDT
                     IDFEVYFDQDYATDDIIYLRVIRTDEAVPEMQGWYRGDTHYHSMFTDNLAEMGFPPDI
                     VAYMAKTLSIDWITITDHSCDYDNYGQSMADNWTQLGNMVLANNQNDTAFKFIRGIEM
                     SVNNSDDHIVHCLAYPSEEAPFSMPYLGDGGGDSFGTEIDLDGLFEELLNCGGFAYAA
                     HPFAEGDALGVFVNGGSWNLGHPGFKQNGEAHTYSGTVICNDISLASDVYTADGMNLK
                     PGLLGFEILNMRNRLSVADEMNNPWNATNDGTDAFGIIDTFDTYHYLYRYRQNLEVYF
                     YMLQKALLFAESENINYKIFQSAGSDAHGSFNYSNTDYSFGVTGSVTDNAIGKFSTLV
                     YCPEGMGNNAENVLMALRNGRTILSEGPLVSIEVTNDALAENYYIGDDASVNYTDLLE
                     WKLNYNYASSGLYGNVNYIKFIVGTKDYLFEFYADTTTDHIHLYDLIAGTLGDDYYNG
                     MFFIMAEIRTSKPETPYIDPMGGFYYAYTNPIWIELYDNTQISEFGGMDSFIYPNPAS
                     DFIDLQLSDNDNDNYEVSIYGINNDLISKSKHNKSNPRIHLANLKDGVYTVKLKSARK
                     VRKQKLVVVK"
     gene            complement(1912..2403)
                     /locus_tag="PLK75_11285"
     CDS             complement(1912..2403)
                     /locus_tag="PLK75_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39870.1"
                     /translation="MKKTLLIMVFFAVANVLHSQNTVKSIGLRGGYAYINGPLVEATL
                     QLEYKFLKRFELDMGMTFNRTYIKSGVCLMHQWRFILGKSWSWYLGGGFSFGYWDYSI
                     GYHDFEDDGFFIGFAPNIGIEYLLPKDRFKLSLDIRPELGLYNNPGEDFSPQAALSLR
                     YNF"
     gene            2584..3471
                     /locus_tag="PLK75_11290"
     CDS             2584..3471
                     /locus_tag="PLK75_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOY39871.1"
                     /translation="MAFLTHEKFLSGKWFKAVLLILTGTFIMAAGFVFFISPYRFAPG
                     GVYGIAIVLHHLAGFPTGLTALCIDIPLTLIGIKILGPRFGWKTVLGFVSLAVFVDLL
                     TFFWGDKPLVEGDALLSALFGGVLVGVGLGLVFKSKATSGGSDIIAMIIAKYTKLPVG
                     QLLIYVDSAIVLIGLFAFQDWKIPLYSWIVIYITGHVIDLIVEGMHYDKSLFIISDKY
                     DEIRLKIINDLKRSGTFISGTGMYGNSEKSIIFTVVNRRELSILKDFIKEIDPKAFVT
                     VIDTREILGEGFKSLNEES"
     gene            complement(3476..3819)
                     /gene="rnpB"
                     /locus_tag="PLK75_11295"
     ncRNA           complement(3476..3819)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLK75_11295"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3929..4477
                     /locus_tag="PLK75_11300"
     CDS             3929..4477
                     /locus_tag="PLK75_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39872.1"
                     /translation="MAFLDKIKNWYGKKQLLNLARLRTEPINCNYNDLHTIGILYDAT
                     NEANFKTVRSFVCKLKESHKEVISLGYVNQLHLEDFHIQPKEFLFFCRKDLNWYFRPT
                     ESSTVDFRKIEFDVLIDLCIEEHLPVRYVLNESPAKLKVGKYSEANINYYDMLIDISE
                     EESVDHLFNQTLHYLKMMNQNK"
     gene            4483..5355
                     /gene="dapA"
                     /locus_tag="PLK75_11305"
     CDS             4483..5355
                     /gene="dapA"
                     /locus_tag="PLK75_11305"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOY39873.1"
                     /translation="MNSLFKGTGVALVTPFNTDKSIDFDALRNVVEYVIAGGVEYLVV
                     MGTTAESPTLSSDEKDAVLAHVIKYNAYRLPIVYGIGGNNTEEVVHKIHSSSLSGVSG
                     ILSVTPYYNKPSQDGIYHHFKTIAEASKLPVILYNVPGRTGCNMSANTCVRLASDFKN
                     IIAVKEASGNFEQIMQIRKNAPSGFLVISGDDAITMPLLACGIDGVISVAANAYPKEF
                     SDMVRAGMTSDFTTARALHYPLLDPIQMMFAEGSPAGVKAFLTHKGLVKNILRLPCYE
                     VSEMLFSKIGNYLK"
     gene            complement(5347..6294)
                     /locus_tag="PLK75_11310"
     CDS             complement(5347..6294)
                     /locus_tag="PLK75_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39874.1"
                     /translation="MKTYPSTSSEIENLIRNKVREGNGLYFFDGKLLEADRKQAYKIS
                     QVISAISNTGEACIYCGITPRRGRASFLSPVAACYDETWLLHEIQSNIQLPVRGMELR
                     RIEMPGQKGFVYCVCFPRINDAPHMFDNKMYYKWYRHKCEPMTEGEVRMHYHASVSPE
                     LDFVGIYNTNGLPVLSDGAIKTISFYPKLLIQNKGGAVEKDYKIEVYLPAELHDIQFI
                     PLQSKLTRHEGLYSVFSVAGVNALYQEEITNPLEIKILVTPENFELFCKEFLSIRVYY
                     SKGVTHHDLKLSDTFTYNGHRLAKDDFNNKNVLPQSELF"
     gene            complement(6344..>7867)
                     /locus_tag="PLK75_11315"
     CDS             complement(6344..>7867)
                     /locus_tag="PLK75_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOY39875.1"
                     /translation="ISEAETGFQLHLMKEMGYDVVALGNHEFDYGPRKLSEIINKSML
                     LGEIPAITCCNLSFQEPSDATSGLASLYEKEIIRRSMIIEKAGFKIGVFGLLGYNAAE
                     VAPNMKPLTCDDALKTAKKVSRFLKTEAGADIVICLSHSGLIPESNGTYSGEDVKLAE
                     NCPYIDVIVSGHSHTCLTEALMVNDVIIVQTGAYGVNVGKLELNIESGKIVNFKYQIT
                     QMNDEIQGDYYIFNEVEKQKKTVDSKVLAPLGLHYNQIIASTTFDLVCDEINNPSGSN
                     LGPFLADAIYYYTNKYSPQGTDVALIATGVIRDKISKGKKGYQTPPDIFRVVSLGEGN
                     DGVPGYPLAKIYLTASELKKVLEILYIAPSMSSSNYCYYSGITVYINPDKGMLNKVRK
                     IEINGEEIDFSKENQKLYGLVANSYMLEFVGLIRKISYGLVKVFPKDADGNRIKDMHT
                     AWIDFDSNKEGVQEGKEWLAVLKFIESFEDCNADNIPDIPDNYRMPVTNVVIETSLK"
BASE COUNT         2261 a         1632 c         1613 g         2361 t
ORIGIN      
        1 gtaccgagac gtttaaattt aatgatgaaa tgaacatttg gataccaccc gtttattacg
       61 aaaaaattga cggaagatgg tggtactttg tttataacat gccatacgaa tcgttcgatg
      121 aatgcggtga caccattgat ttcgaagtgt attttgatca ggattatgca accgatgata
      181 taatttatct tagggtaatt cgcacagatg aggctgttcc tgaaatgcaa ggctggtatc
      241 ggggcgacac ccactatcac agcatgttta ccgacaacct cgcggaaatg gggtttccac
      301 cggatatagt tgcctatatg gcaaaaacac tgagcattga ttggataacg ataacggatc
      361 attcatgcga ctacgataat tacggtcaat caatggcaga caactggaca caactgggga
      421 atatggtcct tgccaataac cagaacgata ccgcgttcaa atttatcagg ggcatcgaaa
      481 tgtcggttaa caattcggac gaccatattg tacattgcct ggcctaccct tctgaagaag
      541 cccccttttc catgccttat cttggcgatg gcggaggtga ttcgtttgga accgagattg
      601 atctcgacgg attattcgaa gaattgctca attgcggagg tttcgcttat gctgcccatc
      661 cttttgcgga aggcgatgca cttggagttt ttgtcaatgg tggatcatgg aatctgggac
      721 atccggggtt taagcaaaat ggtgaggcgc acacttattc cggaacggtc atttgcaatg
      781 atataagcct cgcttctgat gtttacactg ctgatggaat gaatctgaag cctggattac
      841 tgggatttga aattctgaat atgcgtaaca gactttccgt cgcggatgag atgaacaacc
      901 catggaatgc tacaaacgat ggaactgatg cattcggtat tatcgacact ttcgatacat
      961 atcattatct gtaccgttac cggcaaaacc tggaagtata tttttatatg ttgcaaaaag
     1021 ccctgctttt tgctgaatca gaaaatatca attataaaat atttcaatct gcggggtccg
     1081 atgctcatgg ttcattcaat tattccaaca ccgactactc gtttggtgta accggttcag
     1141 ttaccgataa tgcaatagga aaattttcga cactggttta ttgtcctgaa ggaatgggca
     1201 ataatgctga gaatgtactt atggctctcc gcaacggaag aacaatactg agcgaagggc
     1261 ctctggttag tattgaggtt actaacgatg cgctggcaga gaattactac atcggcgatg
     1321 atgcttcggt taattatacc gatcttctgg agtggaaact taattataat tatgcctctt
     1381 caggtttgta tggcaatgta aattatatca agtttattgt tggaaccaaa gattacttat
     1441 ttgaatttta tgctgataca accaccgatc acatacacct ttacgattta attgccggaa
     1501 cgctaggcga tgactactac aacggaatgt tctttattat ggctgaaata cgtacttcaa
     1561 aacccgaaac accttatatt gacccgatgg gtgggtttta ttatgcctat accaatccta
     1621 tctggataga gctttacgat aatacacaaa tttcggagtt cgggggcatg gattcattta
     1681 tttaccccaa tcctgccagc gacttcattg atctgcagct ctcagacaat gataatgaca
     1741 attacgaagt aagtatatac ggcataaaca atgacctcat ttcgaagtcg aaacacaaca
     1801 aaagtaatcc gcgaattcac ttagccaatt taaaagatgg tgtatatacg gtaaaactca
     1861 aatctgcacg gaaagtaagg aagcaaaaac tagtggttgt gaaataggag ctcagaaatt
     1921 gtatcgtaac gataaagctg cctgaggtga aaaatcctcg ccggggttat tatataaacc
     1981 caattcaggg cgtatatcga gcgataattt aaatctatct tttggcaaga ggtattctat
     2041 tcctatattc ggtgcaaaac caatgaaaaa gccatcgtct tcaaagtcgt ggtaaccgat
     2101 gctataatcc cagtaaccaa aggagaaccc cccaccaaga taccatgacc atgatttacc
     2161 gagaatgaac ctccactgat gcataaggca aacgcccgat ttaatatagg tccggttaaa
     2221 agtcattccc atatcaagct caaacctttt cagaaacttg tattcaagct gtaatgttgc
     2281 ctctaccaat ggcccgttga tataggcata ccctccgcga aggccaatcg atttcactgt
     2341 attctgcgag tgcaatacat tagctaccgc aaaaaatacc attatcagaa gcgttttttt
     2401 cataactcag acaggttgca aatatacagt ttttattcat gctaacgaat gtattatatc
     2461 caacctgtgc tcaacagatt taccgaaagt ggttaactaa tcattgcaaa ataatgttgt
     2521 taaacaattt tacattttcg tcaggaatgg tatttttgcc ggctaatttt caaagaaacc
     2581 tatatggcat ttttaactca tgaaaagttc ctatcaggaa agtggttcaa ggcagtactg
     2641 ctgatactta caggtacatt tattatggca gcggggtttg tattttttat ttctccttac
     2701 aggtttgccc ccggcggtgt atatggtatt gctattgtat tgcatcacct ggctggtttc
     2761 ccgaccggct tgacagctct gtgcatcgat ataccactta ccctgattgg tattaagatt
     2821 ctgggaccac gttttggatg gaaaaccgtt ttggggtttg tgtctcttgc tgtctttgtt
     2881 gatctgctta cctttttctg gggcgataaa cccttggtgg aaggggatgc cttgctttcg
     2941 gcattatttg gcggcgtact tgttggcgta gggctcggac ttgtttttaa atcgaaagca
     3001 acatcgggtg gcagcgatat cattgccatg attattgcta agtacaccaa actccccgtt
     3061 gggcaattac ttatttatgt ggactcagcg attgttttaa tagggttatt tgcatttcag
     3121 gactggaaaa tccctctgta ttcatggatt gttatatata ttaccggtca tgtaatcgac
     3181 ttaattgtgg aggggatgca ctacgataaa agcctgttca ttatttcgga taaatacgac
     3241 gaaatcaggc tgaaaataat aaacgacctc aaacgcagcg gaacgttcat ttccggtacc
     3301 ggaatgtatg ggaatagtga aaaatcaata attttcacag ttgtgaaccg aagggaattg
     3361 tccattctga aggattttat caaggagatt gatcccaaag cattcgtaac ggtaattgat
     3421 acacgggaaa tacttggcga aggattcaaa tcgttaaacg aagaaagcta gaaaaaaaaa
     3481 gagcagacct gtaagccgga ttctgtaatc cgcaaagcgg atcctttatc atttatctgt
     3541 gcgacctacc ctccgactcg ggcgggccac cctcaggcgt cggtttactt ggtcttgcaa
     3601 cctgcaggac agacagccag caatgttgcc actgctgctg gtaggctctt accctacctt
     3661 ctcaccattg gtgcagcaag ctgcatctgt tgttttcttc tctgttacca taccttcaca
     3721 gacatcttcc atttcggaag tgcagcgccc tgtgttgtcc ggactttcct ccctgccata
     3781 gttctaccta acatgcaaag cgataaagcg gtctgctcgc agcaaagata cattttcata
     3841 agtaaaacac agcatgtaca ctgaaatttg ttttttttgg caatttctaa ttcaattttg
     3901 tatctttcgc aattaaatta aatctattgt ggcgtttctt gataaaataa aaaactggta
     3961 cgggaaaaaa cagcttctga atctggccag gttgcgcact gagccaatca actgcaacta
     4021 caacgacttg catacaatcg gaatactcta tgacgctacc aatgaagcaa atttcaagac
     4081 agtgagatct ttcgtatgca aacttaaaga atcgcataaa gaggttattt ctcttggtta
     4141 tgtcaaccag ttacatcttg aagattttca cattcaaccg aaagaatttt tatttttctg
     4201 ccgaaaagat ttgaactggt atttccgccc tacagagtca agcactgttg atttccgtaa
     4261 aatagaattc gacgttctta ttgatctatg cattgaggag catcttccgg ttcggtatgt
     4321 tctgaatgaa tctccggcaa agctaaaagt tggcaaatac agcgaagcaa atatcaatta
     4381 ttacgacatg cttatcgata tttcggaaga agaaagcgtg gatcaccttt tcaatcagac
     4441 attgcactat ttaaaaatga tgaatcaaaa caagtaaaac atatgaattc gttatttaaa
     4501 ggaaccggag ttgccctggt aaccccattc aataccgaca agtcaattga tttcgatgcc
     4561 ctcagaaatg tagttgaata cgttatcgct ggtggagttg aatatttggt tgtaatgggc
     4621 accacagccg agtcgccaac cttatcatcg gatgaaaaag acgcggtgct tgctcatgtc
     4681 atcaaatata atgcataccg gcttccgatt gtgtacggta taggtggtaa caataccgaa
     4741 gaggtagttc ataaaataca tagttcttca ctgtcgggag tgagcggcat tttatcggta
     4801 acaccttatt ataacaaacc ttcgcaggat ggcatttatc atcattttaa aaccattgcc
     4861 gaagcttcga agcttcctgt gatactttat aatgttccgg gaaggactgg ctgcaatatg
     4921 agtgcaaata cctgtgtacg cctggcatcg gattttaaaa acattattgc tgtgaaagaa
     4981 gccagtggta attttgaaca gatcatgcaa atacgtaaga atgctccatc cggttttctg
     5041 gtaatttcgg gcgacgatgc cattaccatg cccttgcttg cttgcggcat tgacggtgta
     5101 atttcggtgg cagcaaatgc gtatcctaag gagttttcag atatggtaag ggctggaatg
     5161 accagcgatt ttacaaccgc gcgcgcattg cactaccccc tcctcgaccc tattcaaatg
     5221 atgtttgctg aaggaagccc agcgggtgta aaagcattcc tgacacataa gggtttagtc
     5281 aagaacatcc tgagacttcc gtgttacgag gttagtgaaa tgttgttcag taaaatcggc
     5341 aattatttaa aatagttccg actgaggcaa aacgttttta ttgttaaaat catcctttgc
     5401 cagccggtgc ccgttatagg taaaggtgtc cgataattta aggtcgtgat gggtgacacc
     5461 ttttgaatag taaacccgaa tgctgagaaa ctctttacag aataattcaa aattttcggg
     5521 agtaaccagt attttaattt cgagaggatt tgttatttct tcctgataca aagcattaac
     5581 gcctgccacc gaaaatactg aatacaggcc ttcgtgtcgc gtaagcttcg attgaagtgg
     5641 aataaactga atgtcatgca actcagcagg aaggtaaact tctatcttat agtctttttc
     5701 cactgccccc cctttgtttt gaatgagaag cttagggtaa aaactgatag ttttaatggc
     5761 tccatccgaa agaaccggca gtccgttggt gttgtatata ccaacaaaat caagctccgg
     5821 cgacacagat gcatgataat gcatcctcac ttccccttcg gtcatgggtt cacatttgtg
     5881 ccggtaccat ttatagtaca tcttattgtc aaacatatgc ggagcatcgt ttatgcgggg
     5941 gaagcagaca caatatacaa agcccttctg acccggcatt tcgatacggc gaagctccat
     6001 accccttacg ggtaactgaa tattcgactg tatttcatgc agcaaccagg tttcatcgta
     6061 acacgcggca accggactca ggaaagatgc acgccctctg cggggggtga ttccgcaata
     6121 tatacaggct tctcctgtat ttgaaattgc agaaataacc tgtgatattt tgtaggcttg
     6181 ttttctgtca gcttccagaa gtttcccatc aaaaaaatac aaaccattcc cttctcttac
     6241 tttatttctg attaaattct ctatctcact tgaggtactc ggatatgttt tcatatagtt
     6301 gtcacttaat gtgaatccct tgcttgcaat ttcaacaata gaatcatttc aaactggttt
     6361 cgataactac atttgttacc ggcattctgt aattatcggg aatatcaggt atattgtcag
     6421 cattacaatc ttcgaaactt tcaataaact ttagtacagc aagccactcc ttaccttcct
     6481 gaacgccttc tttgtttgag tcaaaatcaa tccatgctgt atgcatgtcc ttgatccggt
     6541 tgccatcagc atctttgggg aacaccttta caagcccata gctaattttt cgtatcaggc
     6601 caacaaattc gagcatatag gagttggcaa cgagcccata taatttctga ttttctttcg
     6661 aaaaatcgat ttcttcgcca ttaatttcta ttttacggac tttattcagc atccccttat
     6721 ctggatttat atatacagtt atacccgagt aatagcaata atttgaagag ctcatcgaag
     6781 gggcaatata tagtatttcc agtacttttt tcagttctga tgccgttaga taaattttag
     6841 ccaggggata gccgggaacc ccatcatttc cttctcccag tgatacaacc ctgaagatat
     6901 ccggtggtgt ctggtatccc ttcttccctt tcgatatttt atcgcgaatc acaccggtag
     6961 caatcagagc aacatcggtg ccttgaggac tgtatttatt ggtgtaatag tatattgcat
     7021 cagcaagaaa aggccccaga ttgctccctg aagggttgtt tatttcatcg caaacaaggt
     7081 caaaagtggt agaagcaatg atttggttat agtgcaaacc gagtggtgcc agtacttttg
     7141 agtcaactgt ttttttctgt ttttcaactt cgttaaagat ataatagtcg ccttgaatct
     7201 catcattcat ttgagtaatt tgatacttaa agttgactat tttaccactt tcaatattta
     7261 gctcaagttt gcctacgttt actccataag caccggtttg tacgataata acatcgttta
     7321 ccatcagtgc ttcggtaagg caggtatgag aatgtccgct aacaattaca tcaatataag
     7381 ggcaattttc ggcaagctta acatcttcac ctgaataagt cccgttgctt tcggggatta
     7441 aaccgctatg agaaaggcat atcacaatat cggctcctgc ttcggttttc aaaaatctcg
     7501 atactttctt tgctgtttta agggcatcat cgcaggtaag aggcttcata ttgggagcca
     7561 cttcggcagc attgtaaccc agtaaaccaa agactccaat tttaaagcca gccttttcaa
     7621 ttatcattga gcggcggata atttcttttt cgtatagcga agccaggccg cttgtggcat
     7681 cggaaggctc ttgaaacgaa aggttgcaac aggtaatggc tggtatttct cccaaaagca
     7741 tcgatttatt gataatttcg gaaagtttgc gaggaccata atcgaattcg tgattgccca
     7801 gtgccaccac atcataaccc atttctttca tcaagtgcaa ctgaaaaccc gtctcggcct
     7861 ccgatat
//
LOCUS       DAOOQP010000133         7750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11621_length_7750_cov_4.646914, whole genome shotgun sequence.
ACCESSION   DAOOQP010000133 DAOOQP010000000
VERSION     DAOOQP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11621_length_7750_cov_4.646914"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..2353
                     /locus_tag="PLK75_11320"
     CDS             236..2353
                     /locus_tag="PLK75_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39876.1"
                     /translation="MKHKNQHIGKAGKSRPTSGQKLLHKKSNTGGIDRINAKKKIGPL
                     QLIIIFLLPFLLYSQTISFGFTYFDDDGIIIRNIDFLSDAGNIPEAFLTDQFILKDSS
                     FYRPLGTVSYMLDILLSGGNNPWMYHFTNVLLMSLIAVMFFIFLTQFNFTVRTALFGS
                     LIFSVHPLFVSTVAFIPNRAELLVVLFSLMAFSMFIKSLASRKAFHLILHFAAFTLAL
                     FSKESAAFLPVLLLLYYFIFYYKKPFQRKHLLLVFVYLIPGVLWLWLRSGAINQVANL
                     EEKFGLTALLYNLRLFPESLAKFAFPFDSAPIPQFSIPMFIAGIVMIAAIVYFLFKSK
                     GKKKYINLFYLGWFIIIMLPPALFRHPYIDYLDHRFIFPFIGIMMFILTAVTDGRTQR
                     SSPLFKAIPFMLIIAFACMSFIKTLPYSDPQSFYDSAVSKNPKSALAYNNRGFCVKLK
                     NNDYDGAIQDFTAAISINPKYADVYGNRGFAYDQMKMYNLAVADFSAAIQLNPSKSLF
                     YYDRGISYFNMHRYDSAVADLTKAIELSPQIAEYYYNRGIAYVNFDSAYKAITDFTKA
                     IELKPDYKDAYLKRGSCYNSASDYVKAISDFNKVIQTDPGNADAYNNRAKAFVNSGKV
                     EQGIGDYTKAIQLKPESAEYYNNRGIAYAVQGNMDMALSDFSKAIELNPSNKESYFNR
                     GRVYSTMGLHEQANQDFLKAGVQ"
     gene            2384..4222
                     /locus_tag="PLK75_11325"
     CDS             2384..4222
                     /locus_tag="PLK75_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39877.1"
                     /translation="MSKNKPNSKAPLQTGKSIPLKKQPENGLVSKKHWLLLFLIPVLL
                     YSQSLKFGFTDFDDDTIIRNNIGFLGNPANIPKVFTSDAFLAETGHFYRPLQTLSFMT
                     DIQLSGADSTWMFHLTNILLLGFICCALFALIRQFSVTVSAAFYGSLIFACHPLFVFS
                     VAWIPARGDLQLTLFSLLSVLYFMKYLNSTRALHLMLHIVCFTLALFSKETAVALPAV
                     YFTYVVLFGKRKNAKKLIFIFAMYLVIGVLWFWLRSIAMGSAPDNHEFQGFLERTNEV
                     GVIAALTNLQSLPEAIFHFFIPIDIDIIPNYSTIRTAIGLILFGMIVVIFFKNRKKYA
                     KEKLFGLVWFILLLLPAMLYKHNIIDYLHHRFLLPLGGILIFLMFLIPPQWIQKGKIK
                     HSWVLICFLIVAGTMSFIKTRAYSDPMNFYNSAIAQNSSSDFSFNNRGVLFTDQGKFN
                     EAIQDYTKAIEMNPQYYEAFYNRGVAYNTLGMYDDAIADNSRAIELFPEYIDALNNRG
                     VAYYHKGMMDKAIADYSTSIRIKPNAPIAYFNRGIIYHQQGFTEKAMADYTKAIELKP
                     DYDVAYYNRSMIYIAQADNTLACNDLRKAADLGYQPAIESVKTLCK"
     gene            4219..6306
                     /locus_tag="PLK75_11330"
     CDS             4219..6306
                     /locus_tag="PLK75_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39878.1"
                     /translation="MKKQRINKPASKPASHLKQHKKINTGILLLLIVILVIATFSPTF
                     QNDYTNWDDQAQITENPDIQNLSSQSIVEIFSSTYVGMYQPVTTTTYALLHAIFGLNP
                     TAFHSFSLMLHLINVILVFLLISRFTGNKALGLGVCLLFGVTPMQTEVVAWASATSSL
                     LYSMFFLLALLMYIRYVQSGRNVGHYMFSLTLYLIALMSKSAALTLPLVMLACDYFLN
                     RKITKTDLANKIPFLALSVVFGILTIITRKEAGDALAVPEAYTIIDRFFLILFSLTFY
                     IFNLLLPVSLSAFHTYPEKTGNLLPWFYYAYPLLLAGLIFFVWRLKKQRRDIVFGLVF
                     FVLSISVMIDLIPAGEQYVKERYTYIPSIGVFFSLLIYLYSLSERNIRTKKYVTAAVI
                     ALALVFSTLSYARTLTWNNSLALWNNVIEKNPGCSVAFNNRGNLFYDMGMRENALSDY
                     NKAIELRHDYPEAWYNRGIMHFDDNRFNEAINDYSQAITLKPSYAEAYLNRGAAYDRL
                     LLFDKAIADYSKAIAIDSTDYKAYNNRGVSYYNEKKYNQAIEDHGKAIKINPNDPQSY
                     ANRANCYFATSNYIKAIEDYSRAISIHSEYIEALYFRSISYERTGNIDNAISDLTTLI
                     SIKPDMTDAYMNRGILYGKKNMPDNAITDFTKVIELNPGNKAAYWNRGIAFGQKGMQA
                     ESEADFKIAGTLP"
     gene            6336..>7750
                     /locus_tag="PLK75_11335"
     CDS             6336..>7750
                     /locus_tag="PLK75_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39879.1"
                     /translation="MKKTKSKHSKSTASKPLGLSDKWNENKKLTLTIATIVFAALITF
                     APVIFNDFTNWDDNLQVTENADITSLSFKNIGKIFSSYYADMYQPLVTLNFAFIYRFF
                     GLDAMAFHAFSLVLHLVNIMLVFLLTKRLRTSIAVSIGVSLLFALNPLQVEPVAWVSA
                     TSTLLFTMFYLLSIIVYIDYSRNKSQTKVYFISLGLFTLSLLSKSAAVTLPVVLLAVD
                     FFLNSKITRKDIIIKTPFLVLSVVFGMITVFKRGSLGGELQMTDYYTLFQSAVLLLYP
                     LAHYAVSAIVPFNISPYHPYPEVNGGALPLLYYIIPASVLLLAIALIFIKKSRVSIMF
                     GVLFFLFSISVMLAFIPLNSGVVKERYFYLPCIGIYFVLFSVLHDYTSRRKSTRTVSM
                     VAVAGLAVFFAVSSFTGAKVWKNSSTLWTKVIEEYPKAHLAYYNRGTAFFDRQFYLES
                     IDDYKKTIELKPDFAESYLNMG"
BASE COUNT         2229 a         1643 c         1441 g         2437 t
ORIGIN      
        1 aatttctaca acctgtgtcc gggtgaatat ataattcaac tgcaaaccgg aaataggatg
       61 gttaccaata aaatgatggt tgaataaccc atacccctct gttttagaag atgctgcagc
      121 cttgcttgca gcatcttttt tattttagtc aaataatcaa caaacaaata tttttatact
      181 ttagctttta ataattttac ctgttattca aaaacaacaa gtacaaaatc ttggcatgaa
      241 gcacaaaaat cagcacattg gaaaagcagg aaaatcgcgc cctacctctg gtcagaaatt
      301 gcttcataaa aaaagcaata caggcggaat agatcgaata aatgcaaaaa aaaagatagg
      361 cccgttgcag cttatcatta tctttttgct tccctttctt ttgtattcac agacaatttc
      421 gtttgggttc acctattttg atgatgatgg aataataatc agaaacattg actttttaag
      481 cgatgcaggc aatattccgg aggcttttct caccgatcag ttcattttaa aggacagctc
      541 attctaccgg cctctgggca cagtgtcgta catgctcgac atactacttt ccggcggcaa
      601 caacccttgg atgtaccatt tcaccaatgt attgctgatg agcctgatag cagtaatgtt
      661 ttttattttt ctgacgcagt ttaatttcac tgtgcgcaca gcattatttg gatctctgat
      721 attcagcgta cacccgttat ttgtttcaac cgttgcattt atacccaacc gcgccgagct
      781 gctggtggta cttttttcgt tgatggcatt ttcgatgttc attaaatccc ttgcaagcag
      841 aaaagcattc cacctgatac ttcacttcgc tgctttcaca cttgcgcttt tcagcaagga
      901 atcggcagca tttctgccgg ttttgctatt gctctattat ttcatttttt attataaaaa
      961 accatttcaa cgaaaacatt tgctacttgt gtttgtatat ttgattcccg gtgttctctg
     1021 gctatggctg aggtccggtg caattaatca ggttgcaaat ctggaagaaa aattcggatt
     1081 aacggcactt ctttataatc ttaggctttt ccccgaatca ctggctaaat tcgcttttcc
     1141 tttcgacagt gccccaattc ctcagttcag tattcccatg ttcatagcag ggatcgtgat
     1201 gattgcagca atagtctatt ttctttttaa atcgaagggt aaaaaaaaat atataaacct
     1261 gttttatctg ggctggttta taataattat gcttcctccg gctttgttcc gccatccgta
     1321 tatcgattat ctcgatcatc ggtttatttt tccatttatc ggcattatga tgtttatact
     1381 tacagcggta actgatggac gcacacaaag aagttctcca cttttcaaag ccattccttt
     1441 tatgctcatc attgcatttg cctgtatgtc gtttattaaa acccttccat actccgaccc
     1501 gcaaagcttt tacgacagtg ctgtttcaaa aaatccaaaa tcggctttgg cctacaacaa
     1561 ccgcggattt tgcgtaaaat taaaaaacaa cgactatgac ggcgcaattc aggacttcac
     1621 tgcagccatc agcatcaatc caaagtatgc cgatgtttac gggaaccgtg gttttgcata
     1681 cgatcagatg aaaatgtata atctcgcagt tgccgatttt tctgcggcaa ttcaacttaa
     1741 tccaagcaaa tcgctgtttt actacgacag gggtatttcg tatttcaaca tgcatcgtta
     1801 cgatagtgcc gttgccgact taacaaaggc tattgagtta agccctcaga ttgcggagta
     1861 ttattacaac agaggtattg cctatgttaa tttcgactca gcctataagg ctatcaccga
     1921 ctttacgaaa gccattgaac tgaagcccga ttacaaagat gcttatttaa agcggggatc
     1981 ctgttacaac agtgcttccg attatgtaaa agctatttcc gacttcaaca aggttataca
     2041 aactgacccg ggtaatgcgg atgcatataa caatcgcgcc aaggcctttg taaacagtgg
     2101 gaaagtagaa caaggtattg gcgactacac aaaagcaatt caactgaaac cggaatctgc
     2161 cgaatactac aacaacagag gcattgcata cgctgttcag ggcaatatgg atatggccct
     2221 tagcgatttt tcaaaagcca ttgagctcaa tccttcaaac aaagagagct attttaaccg
     2281 aggcagggtg tattctacga tgggcttaca cgaacaggcc aatcaggatt ttttaaaagc
     2341 cggggtacag taaaaccgct agtttcatta gtttattatc tgaatgagta aaaacaaacc
     2401 caatagtaaa gcgcctcttc aaacagggaa atctattcct ctgaaaaaac aacctgaaaa
     2461 tgggcttgtt tcaaagaagc actggctgtt gcttttcctt attcccgtcc tgctctattc
     2521 acagagcctt aaatttggat ttaccgactt tgatgacgat accataattc ggaataatat
     2581 aggattttta ggtaatccgg caaatattcc aaaagtgttt acttctgatg cctttctcgc
     2641 agaaacaggg catttctaca gacctttgca gacactgtcg tttatgacag acatacaact
     2701 atcaggggca gatagtacct ggatgtttca tctgactaac atcttacttc ttggattcat
     2761 ttgctgtgct ctctttgcac taataagaca gttttcggta actgtgagtg ctgccttcta
     2821 cggaagcctt atttttgcat gtcatccatt atttgttttt tctgtggcct ggattccagc
     2881 ccgcggtgat ttgcaactta cacttttttc gcttctatcg gtgttgtatt tcatgaaata
     2941 cctgaatagc acacgggcat tacatctgat gctgcacata gtttgcttta cgttggcatt
     3001 gttttcaaaa gagactgctg ttgcattacc tgcagtgtat ttcacatacg tcgttttatt
     3061 tggaaaacgc aaaaatgcaa aaaagttgat ttttattttt gccatgtact tagttattgg
     3121 tgttctgtgg ttctggcttc gttccattgc catgggcagt gctccagaca atcatgagtt
     3181 tcagggattt ctggaaagaa ccaacgaagt gggtgttatt gctgcattaa ctaacttgca
     3241 aagccttccc gaagcaattt ttcacttctt tatcccaatt gatatcgata ttattcccaa
     3301 ttactcaaca atcagaaccg ctattggatt gatattgttt gggatgattg tagtaatatt
     3361 tttcaaaaac cgtaaaaaat atgctaaaga aaaactcttt ggtctggttt ggttcatact
     3421 gctattactt cctgcaatgc tttacaagca taatatcatc gattatctgc atcaccgttt
     3481 tctgttgcct ttgggaggta ttttgatttt tcttatgttt cttattccac cccaatggat
     3541 tcaaaaaggg aaaattaaac actcctgggt tttgatttgc tttctgattg ttgccggaac
     3601 gatgtcgttt attaaaacgc gtgcatattc cgacccgatg aatttctata attccgccat
     3661 tgctcaaaat tcatcctccg atttctcgtt caacaacagg ggtgtactat tcaccgatca
     3721 ggggaaattt aacgaggcga tacaggatta caccaaagcc attgaaatga acccgcaata
     3781 ttacgaagcc ttctataacc gcggtgtggc gtataataca cttggaatgt atgatgatgc
     3841 catagctgac aattcacgag caatagagct tttccctgaa tatatcgatg cccttaacaa
     3901 ccgcggagta gcatactacc ataaaggtat gatggataaa gccattgccg actacagcac
     3961 ttctatcagg ataaaaccga atgcccccat tgcttatttt aacaggggta taatctatca
     4021 tcagcaagga tttaccgaga aagccatggc tgattacacg aaagccatcg aacttaaacc
     4081 cgactatgac gtggcttact acaacaggtc aatgatttac attgcacagg cggataacac
     4141 attggcttgc aacgaccttc gaaaagctgc cgatttagga tatcaacctg ctatcgaaag
     4201 tgttaaaacc ttatgcaagt gaagaaacag cgaataaaca aaccagcatc caagcctgct
     4261 tcgcacttaa agcagcacaa gaaaatcaat acaggtattt tacttttgct tatcgttata
     4321 ttggttattg caacattttc gcccactttt cagaatgatt atacaaactg ggacgatcag
     4381 gcacaaatca ccgaaaaccc cgacattcaa aacctgtcgt cacaaagcat agtagaaata
     4441 ttttcatcca cctatgtggg tatgtatcaa cctgtaacaa caacaaccta tgcactgtta
     4501 catgcaatat ttggtttgaa tccaacagca ttccacagtt ttagtctgat gctgcattta
     4561 atcaatgtaa ttctggtttt tttgctgatt tcccgtttta cagggaacaa agccttaggt
     4621 cttggcgttt gccttttatt cggagtcacc cccatgcaaa cagaagttgt tgcctgggcg
     4681 tcggcaacaa gcagtttact gtattcgatg ttttttctgc ttgcgctgct catgtatatt
     4741 cgctacgttc aatcggggcg gaatgtcggt cattacatgt tttcattaac cctgtattta
     4801 atcgcactga tgtcgaaatc ggctgctctt accctgccat tggtaatgct tgcatgcgat
     4861 tattttctca atagaaaaat cactaaaacc gatctggcca acaaaattcc atttctggct
     4921 ctttcggttg ttttcggaat tctaactatc attacccgca aagaagccgg agatgcgctt
     4981 gctgttcctg aagcctatac tattatcgat cgatttttcc tgatactatt ctctctcaca
     5041 ttctatattt tcaaccttct actacctgtt agcttatcgg cttttcacac ctatcccgaa
     5101 aaaaccggca atctgcttcc atggttttac tatgcttacc ctttgcttct tgcaggactc
     5161 atattttttg tttggagatt gaaaaaacaa agacgtgata tagtgttcgg gttggtattt
     5221 tttgtgctct ccatttcagt aatgattgat ttgattccgg caggggaaca atatgtaaaa
     5281 gaacgataca cttatattcc ttccattgga gtattttttt cattgctgat ttatttatat
     5341 tccctttccg aaagaaacat caggacaaaa aaatatgtga ctgccgctgt aattgcatta
     5401 gcattggtat tttcaacact tagctacgca aggacactta catggaataa tagtcttgca
     5461 ctatggaata atgtaatcga aaaaaacccg gggtgttcgg ttgctttcaa caaccgcgga
     5521 aatttatttt acgatatggg aatgagagag aacgcactca gcgattataa taaagctatc
     5581 gagctgcgtc acgactaccc cgaagcgtgg tacaacaggg gcataatgca tttcgacgac
     5641 aaccggttca acgaagccat caacgactat tcgcaagcaa tcactctgaa accttcgtat
     5701 gcagaagcct atttaaacag gggtgccgcc tacgacaggt tgctgctttt cgataaagcc
     5761 atagccgatt actccaaagc catagctatc gattctaccg actataaagc ctataataac
     5821 aggggtgtaa gttattacaa cgagaagaaa tacaatcagg cgattgaaga ccatggtaaa
     5881 gctataaaaa ttaatcccaa cgatccacaa tcgtacgcca acagagccaa ttgctatttt
     5941 gccaccagca attacataaa agccatcgaa gattacagcc gggcaatttc aatacactct
     6001 gaatacatag aagctttata tttcagaagc atctcttacg aaagaactgg caacatcgac
     6061 aatgcaatca gcgatttaac cactcttata tcaataaaac cggatatgac tgacgcatac
     6121 atgaaccgtg gaattttata cgggaaaaag aacatgcccg acaatgccat caccgatttt
     6181 acaaaagtca ttgaactgaa tcctggtaat aaggcagcct attggaaccg aggtatagct
     6241 tttgggcaaa aaggcatgca ggctgaatca gaagccgatt ttaaaatagc tggaacatta
     6301 ccctaagttc ttcattgttg aaccatttgt actttatgaa aaaaacgaaa tcaaaacata
     6361 gcaaatcaac agcttcaaaa cccctaggat tgtcagacaa atggaatgaa aacaaaaagc
     6421 tgaccctgac gattgcgact atcgtttttg cagcgttaat aacttttgct cctgtaatat
     6481 ttaatgattt cacaaactgg gacgacaact tacaggtaac cgaaaatgct gatatcacat
     6541 cattgtcatt caagaatatt ggtaaaattt ttagcagcta ctacgcggat atgtatcagc
     6601 cgctggtaac actgaatttt gcatttattt acaggttctt tggtttggat gcaatggcct
     6661 ttcacgcatt cagccttgta cttcatctgg taaatattat gttggtcttc cttcttacaa
     6721 aaagactgcg tacttccatc gccgtctcaa taggcgtaag tttgttattt gctttgaatc
     6781 cccttcaggt agagcctgtt gcctgggtct ctgctacaag cacattgctt tttaccatgt
     6841 tctatcttct ttcaataatt gtatatatcg attattcaag aaacaagtcg caaacaaaag
     6901 tgtattttat ctcactgggg ctattcacct tatcgcttct ttcgaaatcg gctgcggtaa
     6961 cccttccggt ggtgttactt gcagttgatt tttttctcaa ttcaaaaatc acccgtaaag
     7021 atattatcat taaaacacct tttcttgtgt tatctgttgt tttcggcatg ataaccgttt
     7081 ttaaacgggg ttcacttggc ggtgagttgc aaatgactga ttattatacc ttatttcaat
     7141 cggctgttct gttgttgtac cctttggctc actatgcagt tagtgcaatt gtaccattca
     7201 acatttctcc gtaccaccct tatcctgagg ttaacggagg agctctgcca ttgttatact
     7261 atattattcc tgcatcagtg ctgttactag ctatcgcatt gatattcatc aaaaaaagca
     7321 gggtatccat aatgtttggt gtattgttct ttttgttcag catttcggtg atgcttgctt
     7381 ttattccact gaattctggg gttgttaaag aaaggtactt ttatcttccg tgcatcggaa
     7441 tttattttgt tttgttcagc gtattgcacg attatacttc acgcagaaag tctacaagaa
     7501 ccgtttcgat ggttgctgtt gctggtttgg ctgttttttt tgcagtcagc tcttttacag
     7561 gcgcaaaggt ctggaaaaac agtagtacat tgtggacaaa ggttatcgaa gaatacccca
     7621 aagcgcatct tgcctattac aacagaggca ctgcattttt tgaccggcag ttttacctgg
     7681 agtcaatcga cgattataaa aaaactatag agcttaaacc ggatttcgcg gagtcatacc
     7741 tcaacatggg
//
LOCUS       DAOOQP010000134         7585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_11943_length_7585_cov_5.380080, whole genome shotgun sequence.
ACCESSION   DAOOQP010000134 DAOOQP010000000
VERSION     DAOOQP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11943_length_7585_cov_5.380080"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLK75_11340"
     CDS             <1..284
                     /locus_tag="PLK75_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39880.1"
                     /translation="NKNPYIGIVPNQATQAFEIYPNPAKNEVFIKLNGKNNGKSGTFA
                     LYDLSGKKLMHAEDIPKNGRIALPALAKGLYVAEIEVERQKYSRKLQIE"
     gene            466..927
                     /locus_tag="PLK75_11345"
     CDS             466..927
                     /locus_tag="PLK75_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOY39881.1"
                     /translation="MLQRIQTLYLLITAIIMGSILFIPYGEILLKDTQIVKVTSLGTR
                     VTAGDTITVFRTYPVFVIGLLSFLVTIVSIFLYKKRILQIRLCVLNFILVLSTTGLMF
                     LYIYRAAKYFDATVYHGFLMVLPLVAAILLWLTIRAIGKDEALVRSIDRIR"
     gene            complement(997..2208)
                     /gene="rocD"
                     /locus_tag="PLK75_11350"
     CDS             complement(997..2208)
                     /gene="rocD"
                     /locus_tag="PLK75_11350"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOY39882.1"
                     /translation="MTPQEYMDRESKYGAHNYHPLPVVLERGEGPFVWDVEGKKYFDF
                     LSAYSAVNQGHCHPKIVDALCDQARKMTLTSRAFYNNVLGEYEEYVTKYFGYDKVLPM
                     NTGAEADETALKLCRRWGYDKKGIPENQAKIIVCEGNFHGRTITIISMSTDPDSYKGF
                     GPYTPGFITVPYNDLNALAKALEDPNVAGFLVEPIQGEAGVYVPEDGYLKKAYDMCKA
                     KNVLFIADEVQTGIARTGKLLACDHEGVRPDILILGKALSGGVIPVSSVLADDDIMLV
                     IKPGEHGSTFGGFPLAGKVAMAALEVVKNEKLAEKAEYLGKIFRNEMLAAKSDMIGLV
                     RGKGLLNAIVVNPKNGKEAWDVCLEMAKNGLLAKPTHKHIIRFAPPLVITEEQLMDAI
                     AIIKKSFHKFE"
     gene            complement(2423..3640)
                     /locus_tag="PLK75_11355"
     CDS             complement(2423..3640)
                     /locus_tag="PLK75_11355"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY39883.1"
                     /translation="MFTRKLFHIVKEHLNRKEFSIIVGARQVGKTTILRQIEKFLQKS
                     GESVYFISLEDFDVLQELNKHPENIFKFVSKQKSSRMYVLIDEIQYLDKPSNFLKLLY
                     DKYADELKIVATGSSAFYIDKKFADSLAGRKRLFYLPSLDFEEFLIFKKAENLIAEVE
                     LIKQQEDYISLHRNEIQSLFNEFLTFGGYPGVVIESQIEEKHNVLKDLYNSYLKKDVV
                     DAGIQDQPRFYKLLTLLAHQTGSLLNVNEISNTLGLSNTAVNNYIEVLNKSFHISKVS
                     PFSNNIRNELTKMPKIYFSDLGFRNIILNSFNPIDNRIDKGIIVENFIYKALINHYDK
                     DNVKHWRTADGDEVDFVVEAMYSKFAIEAKFNCKQFNPKKYARFNKFYPDIPISCKAY
                     ISDKNSDNVLSLF"
     gene            complement(3928..6024)
                     /gene="scpA"
                     /locus_tag="PLK75_11360"
     CDS             complement(3928..6024)
                     /gene="scpA"
                     /locus_tag="PLK75_11360"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOY39884.1"
                     /translation="MKQVKPSGKAGIQGGWLTPEQIPVKPVYTKDDLLNMEHLNYAAG
                     LPPYLRGPYSTMYVMQPWTIRQYAGFSTAEESNAFYRRNLKAGQKGLSVAFDLATHRG
                     YDSDHPRVVGDVGKAGVAIDSILDMKVLFDEIPLNQMSVSMTMNGAVLPIMAFYIVAA
                     LEQGAKLEELAGTIQNDILKEFMVRNTYIYPPEFSMRIIGDIFRYTSEKMPKFNSISI
                     SGYHMQEAGATNDIELAYTLADGLEYLRTGIRSGLDIDAFAPRLSFFWAIGMNHFMEI
                     SKMRAARLLWAKMVNQFNPKNPKSLALRTHCQTSGWSLTEQDPFNNIARTCIEAMGAA
                     LGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICKQVDPWGGSYYVESLTH
                     AIAHRAWKHIEEIEQLGGMAKAIESGIPKMRIEEAAARAQARIDSGNQTIVGVNKYRL
                     EKEDPIDILDVDNTAVRLAQIERLKRLKAERNEAECQAALKAITRSAETGEGNLLELA
                     VIAAQKRATLGEISDACEKIAGRYKAVIRSISGVYSSEAHNDPDFEKARALCEQFAKK
                     EGRQPRIMIAKMGQDGHDRGAKVVSTGYADIGFDVDIGPLFQTPEEAARQAVENDVHI
                     LGVSSLAAGHKTLIPAVIEELKKLGREDIMIVAGGVIPAQDYAFLYENGVAAVFGPGT
                     SVAKAAVQILELLLQD"
     gene            complement(6084..>7585)
                     /locus_tag="PLK75_11365"
     CDS             complement(6084..>7585)
                     /locus_tag="PLK75_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOY39885.1"
                     /translation="CTPLTYQQLETLLNGIDQTTVPVHIVGNGSDSSVPGMMAEFAAK
                     HQIDPNAIKGSSGFDPLAYLMLSGKTPCGTDSCQCVESAFAKAGNTLPNFKLLNINAR
                     HIHNAGGSIVQELATGLAMGAEYLQRLTDAGHSIDDVAPRMLFVFAQGSNYFMEIAKL
                     RAARLLWSAICDEFNPKSADSGHMCIFAETSEWNKTIYDPYVNILRATTEAMSGALGG
                     ASAICVQPFDAVYACSDDFSERIARNIQIILKEEAHFDKVNDPAAGSYYIEHLTKAIA
                     EEAWKLFLQIQELGGFTPAFRQEFIQKFIDETLAKRNSNAETRREIILGTNQFPNFNE
                     KVDASKLKKPHPAIEGGIRPIRLHRAAEAFEAMRLKTDSAAREINVFMLKIGNVAMRQ
                     ARAQFAANFFACAGFRIIDNLGYETVAEGVNDALAAKADIVVMCSSDDEYALLAPEML
                     SILGNKAICVIAGNPACRPELEAAGISNYIHVKSNLLAELKQYQQKLGL"
BASE COUNT         2086 a         1650 c         1585 g         2264 t
ORIGIN      
        1 gcaacaaaaa cccgtatatc gggattgtgc ccaaccaagc aacgcaggca ttcgaaatct
       61 accccaaccc ggctaaaaac gaagtgttca tcaaacttaa tggtaaaaat aacggcaaat
      121 cgggcacttt cgcactgtat gaccttagtg gaaaaaagct catgcatgca gaagatattc
      181 ctaaaaacgg ccgcattgca ctgcctgcgc tggcgaaagg cttgtatgta gccgaaattg
      241 aggtggagag acagaagtat tcgagaaaat tgcaaattga gtaaatatct taacccccta
      301 aatagctgca ctaaattaaa tcttgcaatt ctatcgtttc caatacaaaa agctgctttt
      361 aaagtagctt gtagccaatt tcaattagaa gaaatgagat taaaagtctt ccgtttaatt
      421 agccttgttt ttgaaaaaca gttattacat ttgtaaaaaa aaactatgct tcagcgaata
      481 cagacattat acctgctgat aaccgcaatt ataatgggat cgattttgtt tattccttat
      541 ggggaaattt tattgaaaga cacccagata gttaaggtaa ccagtttggg cacccgtgtt
      601 acagcaggtg atactattac tgttttcagg acatatcctg tttttgtgat tggcttgtta
      661 agttttctgg ttactatagt aagtattttt ctgtataaaa aacgaattct gcaaatcagg
      721 ttatgcgtgc ttaattttat actcgtactt tctacaacag gattgatgtt cctgtatatt
      781 tacagggctg caaaatattt cgatgcaacg gtttaccatg gttttctgat ggttctgcca
      841 ttggttgcag caatcctgct ttggctgact atccgtgcta ttggcaaaga tgaagccttg
      901 gtccgttcga tcgacaggat acgatagcac atgacaataa ttacaaaaaa aaagagccct
      961 gcattattgc cgggctcttt ttttagtcac atatttctat tcgaacttat gaaatgattt
     1021 tttaatgata gcaatagcat ccatgagttg ttcctcggta atcaccagcg gcggagcaaa
     1081 gcggatgata tgcttgtggg ttggttttgc gagtaatccg ttcttggcca tttctaaaca
     1141 aacatcccat gcttcttttc cgtttttcgg gtttacgact attgcattca gcaagccttt
     1201 accacgaacg agtccaatca tgtctgattt cgctgccagc atttcgtttc tgaaaatttt
     1261 acccagatat tctgcttttt cggcaagttt ttcgtttttc actacttcga gagctgccat
     1321 agccactttt cctgccaatg ggaaaccgcc aaaggtggaa ccatgttcac cgggtttaat
     1381 cacgagcatg atgtcgtcgt cggctagcac tgatgacaca ggaattacac ctcccgaaag
     1441 agctttaccc aaaatcagga tgtcgggacg aacgccttca tggtcgcatg caagcaattt
     1501 acctgtacgg gcaatgccag tctgtacttc gtcagctatg aaaagcacat ttttggcttt
     1561 acacatatcg taagctttct tcaggtatcc gtcttcagga acatatacac cggcttcgcc
     1621 ctgaatgggt tcaaccagaa atcctgcaac atttgggtct tcgagtgctt tggcaagtgc
     1681 gttcaaatcg ttgtaaggaa ccgtaataaa tccaggagtg tatggaccaa aacctttgta
     1741 ggagtccgga tcggtactca tcgaaatgat tgtgatggta cgaccatgga aattaccttc
     1801 gcaaacaatg atttttgcct gattctcagg aatacctttt ttatcgtaac cccatctgcg
     1861 gcaaagctta agtgcggttt catcagcttc cgcaccggtg ttcatcggta gcactttgtc
     1921 gtaaccgaaa tatttggtaa cgtactcttc gtactcaccc aatacattat tataaaatgc
     1981 acgcgatgtc agtgtcattt ttcgagcctg atcacataga gcatcaacaa tcttaggatg
     2041 acaatgacct tgatttacag ctgaataagc tgaaagaaag tcgaaatatt tttttccttc
     2101 tacgtcccaa acaaacggac cttcgcctcg ttcgagaaca accggtagtg ggtgataatt
     2161 gtgggcaccg tatttcgatt ccctgtccat gtactcctga ggagtcatct tttttgtttc
     2221 aatcataatt ataattattt ggttatgttt ttttcaactt gtcaaaaaaa gcaaatttta
     2281 ttgtcaaaag caaacagcta tataacaggt ttaaaaatat tgagcagaga attgagtgta
     2341 tactatagcg cactttattc cctatcggag tggttagtga cagtctgtaa agaagatgaa
     2401 aagggagcca aaccgatttc actcaaaaca aacttaaaac attatccgag tttttatcgc
     2461 ttatatatgc tttgcaagaa attggaatat ccggataaaa tttattaaag cgagcgtatt
     2521 ttttgggatt aaattgtttg caattaaatt tggcttcaat cgcaaatttc gaatacattg
     2581 cttcaacaac aaaatcaacc tcgtctccat cggctgttct ccaatgcttt acattgtctt
     2641 tgtcgtaatg atttattaaa gctttataga tgaaattttc tacaataatg cctttatcaa
     2701 tacggttatc tatgggattg aaggagttaa gaatgatatt cctaaaaccc aaatcagaaa
     2761 aataaatttt aggcatttta gtaagctcat ttctgatgtt gtttgaaaaa ggactaactt
     2821 ttgagatatg aaaagattta tttaaaacct caatataatt gttaacggct gtatttgaca
     2881 atcctaaagt gtttgatatt tcatttacat ttaataatga tccggtttgg tgagcaagca
     2941 aagtcaaaag tttataaaat cttggctggt cttgtattcc tgcatcaact acatcttttt
     3001 ttaaatatga gttatataaa tcttttaaca cattatgctt ctcttcaatc tgactttcaa
     3061 ttactacccc aggatatcca ccaaaagtaa ggaattcatt aaacaaagat tgtatttcgt
     3121 tacgatgcaa cgaaatataa tcttcctgtt gctttatcaa ttctacctca gctataagat
     3181 tttcggcttt tttaaaaatg agaaactctt caaaatcaag acttggtaag taaaacaacc
     3241 tctttctgcc agcaagcgaa tccgcaaatt tcttatcaat ataaaaggca ctacttccgg
     3301 ttgccacaat ttttaattcg tcagcatatt tatcatacaa cagcttcaga aaattcgatg
     3361 gtttatctaa atattgaatt tcatcaataa gaacatacat tctagaagac ttctgtttag
     3421 aaacgaattt gaaaatattt tccggatgct tattcagttc ttgtaataca tcaaaatctt
     3481 caaggcttat aaaataaacg gattctcccg acttttgcaa gaacttctca atttgccgca
     3541 aaattgtagt ttttccaacc tgtcgtgctc caacaataat ggaaaactct tttctgttca
     3601 aatgctcttt tacaatatga aatagttttc ttgtaaacat ggtaaatagt ttttataaag
     3661 ataaatttta tttcagaaat gaaaaatatc cagataattt ttacttttga agtaaataat
     3721 ttcttttttt gattatgcct tcacataaac ctcaatgtcg taaacaaatt aatgtattac
     3781 aatcgtgcgc aaagaattga atatccactg tattcgtccg gggttttaac tccatctgaa
     3841 tcttctgcga atgacccgga tggagaattc aaaataaacg ggattgccaa atgaagaatt
     3901 tgacaacccc gtcagataat caatgaatta gtcctgcagc aataattcaa gtatctgaac
     3961 agccgctttg gcaaccgatg ttccgggacc aaaaactgca gcgacaccat tctcatacag
     4021 gaaagcgtaa tcctgagcag gaataacacc acctgccacg atcataatgt cctcacgacc
     4081 aagcttttta agctcttcaa ttacagcagg tattaaagtc ttatgtcctg ctgccagcga
     4141 agaaacaccc agtatgtgaa catcgttttc cactgcctga cgggcagctt cttcgggagt
     4201 ttgaaacaat ggacctatat caacgtcgaa gcctatgtcg gcatagccgg ttgaaaccac
     4261 tttggcgccg cggtcgtgcc catcttgccc cattttggca atcataattc ggggctggcg
     4321 gccttctttc ttcgcgaact gttcgcacaa tgcccgtgct ttttcgaagt cggggtcgtt
     4381 gtgtgcttct gaggagtaca ctcctgatat tgatcgtatc acagctttat acctccctgc
     4441 tattttttca catgcatccg aaatttctcc caaggtagct cttttttgtg cagcaatcac
     4501 cgccagttcg agcaaatttc cttcaccggt ctcggcactg cgggtaatag ccttcagtgc
     4561 agcctgacat tcagcttcgt tgcgttcagc tttgagcctt ttcaatcttt cgatctgagc
     4621 cagacgcaca gccgtattgt ccacatcgag aatatcaatc gggtcttctt tttcgagacg
     4681 gtatttgttt acgcccacaa tggtctgatt gccactgtcg atacgtgcct gagcgcgtgc
     4741 agcagcttct tcgatgcgca ttttgggaat acccgactcg atggcttttg ccataccacc
     4801 cagttgctca atttcctcga tgtgcttcca tgcacgatga gcaatggcat gggtgagcga
     4861 ttctacataa taactaccac cccatgggtc aacctgtttg cagatattgg tttcttcctg
     4921 cagataaatt tgggtattgc gggcaatacg agccgaaaaa tcggtgggta aagcaatggc
     4981 ttcatccaga gcattggtat gtagactttg tgtatgaccc agagcagcgc ccatggcttc
     5041 gatacaggta cgggcaatat tattgaacgg atcctgctcg gtgagcgacc aaccggaggt
     5101 ctgacaatgg gtgcgcagtg ccagcgattt aggattcttg gggttaaact gattaaccat
     5161 ttttgcccac aacagacggg ctgcacgcat tttggaaatt tccataaaat gattcatccc
     5221 aatggcccag aaaaacgaca atctgggtgc aaaagcatcg atgtcgaggc ctgagcgaat
     5281 tccggttcgg agatactcaa gaccatctgc aagagtataa gccaattcaa tatcgttggt
     5341 agcaccggct tcctgcatgt ggtatcccga aatggagatg ctgttgaatt tcggcatttt
     5401 ttcgctggta tagcggaaaa tatcaccgat aatacgcatc gaaaattccg gaggataaat
     5461 atatgtgttg cgaaccataa attccttcag aatgtcgttc tgaatggttc cggccagctc
     5521 ttcgagcttt gctccctgtt ccagggcagc cacaatgtag aatgccatga tgggaagcac
     5581 agccccgttc atggtcatcg aaacgctcat ctggttcagc ggaatctcat cgaacagcac
     5641 tttcatatcg aggatgctgt cgatggcaac accggcttta cccacatcgc ccaccacacg
     5701 cggatggtcg ctgtcgtagc cacggtgtgt agccaggtcg aaagctaccg aaaggccttt
     5761 ctgaccggct ttcaggtttc tgcggtaaaa agcattcgat tcttcagctg ttgaaaagcc
     5821 ggcgtactgt ctgattgtcc acggctgcat cacatacatg gtggaataag gtccgcgcag
     5881 gtaaggaggc aatccggcag catagttcag atgctccatg ttcagcaggt cgtcttttgt
     5941 atagacaggt ttcactggta tctgttcggg ggtaagccac ccgccctgaa taccggcttt
     6001 gcccgagggc tttacctgct tcataaaatt tgttttttta taatcgggtt tcatattgat
     6061 tctgttttaa tgcaacaatc gttttataat cccagctttt gctgatattg tttcaactcg
     6121 gcaagcaggt tcgactttac atgaatgtaa ttgctgatgc cggctgcttc aagctccggt
     6181 cggcaggcag ggttgccggc aattacgcaa atggctttat tgcccaatat gctgagcatt
     6241 tcgggagcca agagagcata ttcatcgtcg ctgctgcaca ttaccacaat atcggctttg
     6301 gctgccaggg catcgttgac gccttcagcc acggtttcat atccaagatt gtcgataatg
     6361 cgaaatcctg cacaggcaaa aaagttcgct gcaaattgtg cacgtgcctg acgcatagca
     6421 acgttgccga tcttcagcat gaacacatta atctcgcgtg ctgcactgtc ggttttcagg
     6481 cgcatggctt cgaatgcttc agcagcacgg tgaagacgaa tgggtctgat gccaccttcg
     6541 attgcaggat gtggcttttt cagctttgaa gcatccactt tttcgttgaa attcggaaac
     6601 tgattggtac ccagaataat ctcccttcgg gtttcggcat tgctgttgcg tttggctaag
     6661 gtctcgtcaa taaacttctg aataaattcc tgacggaaag ccggagtaaa tccacccaat
     6721 tcctgtatct ggaggaagag tttccatgct tcttcggcaa tggctttggt gaggtgctca
     6781 atatagtaac tgccggcagc cggatcgttt accttatcga aatgggcttc ttccttaaga
     6841 ataatctgga tgttgcgggc gatgcgttct gagaaatcat cgctgcaggc atacactgca
     6901 tcaaaaggct gaacacagat ggcagatgct ccgcccagcg ctcccgacat ggcttcggtg
     6961 gttgccctga ggatgttcac atacgggtcg taaatggttt tattccattc ggatgtttcg
     7021 gcaaaaatgc acatgtgtcc ggaatctgcc gatttgggat tgaactcgtc gcaaatggcc
     7081 gaccacaaca agcgggcagc cctgagtttg gcaatttcca tgaaatagtt ggatccttgc
     7141 gcaaaaacga acaacatgcg cggcgcaacg tcgtcgatac tatgtccggc gtcggtaagg
     7201 cgctgcaggt attcggcacc catggcaaga ccggtagcca gttcctgtac aatgctgcct
     7261 ccggcattat gaatatggcg tgcgttgata ttcaataact taaaattcgg cagggtgttc
     7321 ccggctttgg caaatgcgct ttcaacacat tggcatgagt cggtgccgca gggtgttttt
     7381 ccgctaagca tcaaataggc cagcgggtcg aatcctgatg aacctttaat tgcattaggg
     7441 tcgatctgat gtttggctgc aaattctgcc atcatacccg gaaccgacga gtcggaacca
     7501 ttacctacta tatgcaccgg aactgtggtc tggtcgatgc cattaagtaa tgtttcgagt
     7561 tgttgataag tgagtggagt gcagg
//
LOCUS       DAOOQP010000135         7505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_12125_length_7505_cov_6.423087, whole genome shotgun sequence.
ACCESSION   DAOOQP010000135 DAOOQP010000000
VERSION     DAOOQP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12125_length_7505_cov_6.423087"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..2374
                     /locus_tag="PLK75_11370"
     CDS             302..2374
                     /locus_tag="PLK75_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39886.1"
                     /translation="MGNLNELKVSMKLKKILLLIIVLIVVALIYSNHFRNPFHFDDAH
                     TIESNMMIRDLGNTSKFFTDATTFSSNPTNQSYRPLITLSFAIDYYLAGGLNPVYFHA
                     DMMLVFLLQIILVYFLFLALIKTTKADKNPLAILFFMIAWYGFHVANAETLNYISARS
                     DLYSSFFVVAGLCMYIYFPVLRKWQLYLIPVVLGMLSKEQSAMFAPILFFYILFFELQ
                     MDLPGIFRKKNFSILVKAFVRTLPALIVCVIMSVIILSKQADTFNASDTPLYNYIITQ
                     PWVYLRYFIAFLLPVNLSADSDWVAFKSIFDERAIVGFLFIGILIYASFRCSINRNWR
                     PVSFGILWFLLALIPTSSVIPLAEVTNDHRMFFPFVGLAFAVVWSMYLLIETVSVRIK
                     AAKTLKLIFGIVCICILTGNAYGVYQRNKIWSSEETLWYDVTLKSPNNGRGLMNYGLT
                     QMWKGNYQVANDYFNKALVFSPYYPYLHLNMGVLQKNMGNVAEAENCYKKAIQYGSEF
                     YLSYYYYASFLSDQNRNAEAVGYAETSLRLSPNYLQNRHLLASIYVKLNETQKLRMLL
                     QETLRLFPDDAETKELMKLGSTGLPLPVQLALQKAQSVPTAENYLNLSLEYYNNRMFA
                     ECIDACQQSLKIKPDYAMAYNNICSAYNSLGEWDKAIEAGEKALAIDPNYTLAANNLA
                     LARKNKSK"
     gene            complement(2378..3823)
                     /locus_tag="PLK75_11375"
     CDS             complement(2378..3823)
                     /locus_tag="PLK75_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39887.1"
                     /translation="MKVKTRMDKNRIHIIYPPISKQERYSSDIGNAGGDQIPLGIYYL
                     ASTLLQHGFQVKVTDAEALNLNAKQLTEQILAFEPAYVGISSTTVAFHRALELATEIK
                     AMFPDIIIILGGPHVSANPEHAMASGFFDYGVLGEGEITLPELLKALNHGSGVENIHG
                     LAFKTNNGMVTLTEKRAFIGDLDMLPFPAYHLIEDISLYTPPPSNYKTLPVINIITSR
                     GCPGKCTFCDKNIFGNTCRRRSAENIVEEIKLLNTQYHVNEIAFVDDTFLLDKKRVYR
                     LFELLDAEGIHFPWTCMSRINDVTDEYLLFLKKHGCWHISFGIESGNEEILKLIRKHI
                     SLQKTENVIALCRKYGIKTKGFFIIGHPGDTLQTIDETIRFACRLKLDDIVVTLNTPI
                     PGSPQYSEADAYGSLDKADWNNFNYWRPVFVPIGLDQGTLLRKHREIYRRFYLRPSKL
                     WRYFLSFFGKGGGKRFITILKASLFLLKKSS"
     gene            complement(3792..4187)
                     /locus_tag="PLK75_11380"
     CDS             complement(3792..4187)
                     /locus_tag="PLK75_11380"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOY39888.1"
                     /translation="MDSFSLRIQILSIVVSLLLLAYSARLIIRGKLRAEYAIFWLAIT
                     GTLVIFSIWRDGLQFFSDLLGVYDAPNLVFLVALFGVFLYILHFSIVISKLKDNNKKL
                     SQEIALLREIIEKKEKTSGNESKNEDGQE"
     gene            complement(4180..4908)
                     /locus_tag="PLK75_11385"
     CDS             complement(4180..4908)
                     /locus_tag="PLK75_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY39889.1"
                     /translation="MKTLAIVIPAYNEEKSVAAVVESVNNIRLPNVEITPVVINDCST
                     DNTQHIIASLKCVSLNLPCNLGIGGAVQTGYIYAWENGYDYAMQMDGDGQHPASEIPA
                     FVAAMQTDNAPDVVIGSRYIHRQGFQSSKIRRLGIQYLGFIIRILTRYRITDCTSGFR
                     MINRRALSVVCELYPDEYPEPEAILIFRNAGLKIREIPVTMQERIGGKSSIRDIASLY
                     YIVKVSLAIVFTFFNKQIKIKHHG"
     gene            complement(4963..5616)
                     /locus_tag="PLK75_11390"
     CDS             complement(4963..5616)
                     /locus_tag="PLK75_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOY39890.1"
                     /translation="MYKFVFIFIVLFPCALFSQTTSVRIGLLKYKGGGDWYANPTSLP
                     NLIKFCNENLNTNIQAEYVNVEPGSADIFSCAFIHMTGHGNVIFSDYEAENLRIYLLS
                     GGFLHIDDNYGMDEYVRREMKKIFPDIEMVELPFDHPIYHSRFDFPNGLPKIHEHDNK
                     APQGFGIVHDGRLLVFYSYECDLGDGWEDQSVHHDSDEARLKALQMGANIITYVFSQ"
     gene            5714..5802
                     /locus_tag="PLK75_11395"
     tRNA            5714..5802
                     /locus_tag="PLK75_11395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5748..5750,aa:Ser,seq:gct)
     gene            6043..7272
                     /locus_tag="PLK75_11400"
     CDS             6043..7272
                     /locus_tag="PLK75_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOY39891.1"
                     /translation="MEKKNTFTMLVYMKKTRKDKNGKSPLYLRISYNSNQATITLHES
                     WDEELWSTNMGMAIGNDKFAKNLNRYLTEVKSNVYDHFKRLRDNSKAFSVDDIRDAYL
                     GIETKKGRTILEVFEEHNEKARALKSIDFAPATVQRYEVSKMHTRDFMIWKYKKDDLL
                     LTDITWEFLGDYELYLKTVRNCAHNTALKYLKNFKKIVRIAMATGELTYDPFANYKMK
                     LKPVDRGFLTEEELDKIIKKDFKNDRLDQVRDCFLFSCFTGLAYADIHDLIDENIVIG
                     TDGGKWIKIKRKKTSNLSSIPILKITQKIIDKYSDHPECIKSKRLLPVKSNQKMNCYL
                     QEIADVCKIDKHLTSHVARHTFATTVTLNNNVPIETVSKMLGHSSIEMTRVYARLLDK
                     KVGQDMKHLDGKYDMAV"
BASE COUNT         2285 a         1471 c         1466 g         2283 t
ORIGIN      
        1 aagccagact tatgcgctgg cttctttaaa agtttttatc ggacatcagt gattttcaat
       61 ttaatatttt catcaataat agtctaaaaa aagccaattg tatacaaaat attttaaatg
      121 tgaattttgc aatattatta ttaacaataa ccctattcac acttttacaa aagtagttaa
      181 tacttttaaa tgagtttatt ttaaatcgta tttattttta aaataatata actacaatat
      241 attgatttga taataaagaa aaacattata tttttggaca aatagaaaat attgaatttt
      301 tatgggaaac ttgaatgaac tgaaggtatc gatgaagtta aaaaaaatct tattattaat
      361 tattgtcctc attgttgtag ctttaattta ttcaaatcat tttcggaatc cttttcactt
      421 cgacgatgct catactattg agtcgaatat gatgatcagg gaccttggta atacttcaaa
      481 gttttttact gatgcgacta ctttcagctc taatccaacg aatcaatcgt accgcccttt
      541 gattacatta tcatttgcta tcgattatta tttggcaggt ggactcaatc cggtttattt
      601 tcatgctgat atgatgctgg tatttttgct acagataata ttggtgtatt ttttattcct
      661 tgctctaatc aaaacaacca aagcagacaa gaacccgcta gcgattttgt tttttatgat
      721 tgcatggtac ggatttcatg ttgctaacgc tgaaacattg aactacatca gtgcaagatc
      781 cgatttgtat tcaagctttt ttgtggtagc aggtttgtgc atgtatattt attttccggt
      841 tttacgaaaa tggcagctct acctgattcc tgttgtgctt gggatgcttt cgaaagaaca
      901 gtctgcaatg tttgcaccaa ttttgttttt ttacattttg tttttcgagt tgcaaatgga
      961 tttgcccgga atctttcgaa aaaagaattt tagcatactt gtaaaagcat ttgtaaggac
     1021 attgccggca ttaattgttt gcgtaatcat gagcgttatt atactcagta agcaggccga
     1081 cacatttaac gcaagtgata cccctttata taattacatc attactcagc catgggttta
     1141 tctaaggtat tttattgctt ttcttttgcc ggttaattta tcggccgaca gcgattgggt
     1201 tgcattcaaa agcatcttcg acgaaagggc tattgttgga ttccttttta taggaatatt
     1261 aatttatgca tcattcaggt gttcaatcaa tagaaattgg cggcctgtaa gtttcggtat
     1321 attatggttc cttttagctc ttatccctac ttcaagtgta atccctcttg ccgaagttac
     1381 caacgatcac cgcatgtttt tcccatttgt cgggttggct tttgcagttg tttggtcgat
     1441 gtatttatta atcgagactg tttcagtaag gataaaagcg gcaaaaacgc ttaaacttat
     1501 ttttggcatt gtttgcattt gtattttaac cggaaatgca tacggagttt atcagcgtaa
     1561 taaaatatgg agcagcgaag aaacactctg gtatgatgtt accttaaaaa gcccgaataa
     1621 cggtagaggt ttgatgaatt atggcttgac gcaaatgtgg aaagggaatt accaagtagc
     1681 aaacgattac tttaataaag cattggtatt ttccccttat tatccttatt tgcatctcaa
     1741 tatgggtgtt ttgcaaaaaa atatgggtaa tgttgccgaa gcagagaatt gctataagaa
     1801 agcaattcaa tatggaagcg agttctattt atcgtattat tattatgcat cgtttttatc
     1861 ggatcaaaac cgaaatgctg aagctgttgg ttatgcagaa acatcccttc ggctgagtcc
     1921 gaattacctg caaaaccggc atctgcttgc cagcatatat gtaaaactga atgagaccca
     1981 aaagctcaga atgctgttgc aggaaacctt gcgactgttc ccggacgatg ctgaaacaaa
     2041 ggaactaatg aaactcggat ccactggatt accattgcca gtgcagcttg ccttgcaaaa
     2101 agcacagtcg gtaccaacag ccgaaaacta tctgaattta agcctcgaat attataataa
     2161 ccggatgttt gcagagtgca ttgatgcctg ccagcaatcg ttgaaaataa aacctgatta
     2221 cgctatggca tacaataata tatgcagtgc ttacaattcg cttggtgaat gggacaaggc
     2281 gattgaagcc ggtgaaaaag ctctggccat tgacccgaac tatacattgg ctgccaataa
     2341 cctggctctg gcacgaaaaa acaaaagcaa ataaaattta cgaagatttt tttaacaaaa
     2401 aaaggctggc tttcaggata gttataaacc gtttacctcc gcctttccca aagaaactta
     2461 agaaatacct ccagagtttt gacggccgta ggtaaaatct acggtaaatt tcacgatgct
     2521 tacggagtaa tgttccttga tcaagcccaa tgggtacaaa aacggggcgc caatagttaa
     2581 aattattcca gtcggcctta tcaaggctcc cataagcatc agcctcagaa tattgcggcg
     2641 atccgggaat gggcgtattc agtgttacca caatatcgtc gagctttagg cggcatgcaa
     2701 agcgaatggt ttcatcgatg gtttgcagtg tatcgccggg atgcccgatt ataaaaaagc
     2761 ctttggtttt gattccgtat ttcctgcata gtgcaattac gttttcggtc ttctgcagag
     2821 agatatgctt tctgataagt ttcagaattt cttcattgcc cgattctatg ccaaaggaaa
     2881 tatgccaaca gccgtgcttt ttcaaaaaca acagatattc atcggttaca tcatttattc
     2941 ggctcatgca tgtccagggg aagtgaatgc cttctgcatc gagaagctcg aaaagacggt
     3001 acaccctttt tttgtcgagc agaaaagtat cgtcaacaaa agctatttcg tttacatgat
     3061 attgcgtatt aagcaacttg atttcttcta caatattctc ggcactgcgt ctgcggcatg
     3121 tattaccaaa gatgttttta tcgcaaaaag tacactttcc ggggcatccg cggctcgtaa
     3181 taatattgat aaccggcaat gttttataat tcgacggggg aggagtgtaa agcgagatat
     3241 cctcaataag atggtatgcc gggaatggta acatgtctag gtcgccgata aatgcccttt
     3301 tttctgttag tgttaccatt ccattgttgg ttttaaaagc caatccatga atattctcaa
     3361 ctcccgagcc atgatttaaa gccttaagca actccggaag cgtaatttcc ccctcgccaa
     3421 gcactccata atcgaaaaat cctgacgcca tggcatgttc agggtttgca ctcacatgcg
     3481 gacctcccag aatgataatg atgtccggaa acatcgcttt aatttcggtt gccagctcca
     3541 aagcacggtg aaaggccact gtggtagagc tgatgccaac gtaagcgggt tcaaatgcca
     3601 aaatttgctc tgtcagttgc ttggcgttta aattcagtgc ttcggcatcg gtaaccttaa
     3661 cttgaaaacc atgctgaaga agggttgaag caaggtaata aatacccaga ggaatttggt
     3721 cgccacctgc attgccaata tccgacgaat acctttcctg cttcgaaatg ggtggatata
     3781 taatatgtat cctattcttg tccatcctcg tttttacttt cattacccga tgtcttttct
     3841 tttttttcga ttatctctct gagcagtgca atttcctgag aaagcttttt attattgtcc
     3901 ttcagctttg aaatgacaat ggaaaaatgc agtatataga gaaatacacc aaacagtgca
     3961 accagaaaaa caaggttagg cgcatcgtac accccaagca ggtcggagaa aaactgtaat
     4021 ccatcgcgcc agatagagaa tatcaccagt gtaccagtaa ttgccagcca gaaaatggca
     4081 tattctgcgc gcagtttgcc tctgatgata agccgtgcac tgtatgccag caagaggaga
     4141 cttacaacaa tgctaagtat ttgaatccgt aacgaaaaac tatccatggt gttttatttt
     4201 tatttgttta ttgaagaaag taaacactat tgccagcgac acttttacga tataatacag
     4261 cgatgcaata tcgcgtatcg aagactttcc acctattctt tcctgcatgg ttactgggat
     4321 ttcgcgtatt ttcaaccctg cattgcgaaa gatgagaata gcttccggtt cggggtattc
     4381 gtcggggtaa agttcgcaga ccacactcag ggctctgcgg tttatcattc tgaaacccga
     4441 cgtgcaatct gtaatgcgat aacgggttag tattcggatg ataaatccaa ggtattgtat
     4501 tcccagccgg cgtattttcg acgattgaaa cccctgccgg tggatatagc gagaaccaat
     4561 aaccacatcg ggcgcattgt cagtttgcat ggctgccaca aatgcgggta tttccgatgc
     4621 aggatgttgt ccgtcgccat ccatttgcat cgcataatcg taaccgttct cccatgcgta
     4681 aatatatccc gtctgtacag cacctccaat tccaagattg cagggtaaat tcaggcttac
     4741 acatttgagc gaagctatga tatgttgtgt attgtcggtt gaacaatcgt taattactac
     4801 aggggttatt tctacattgg gcaacctgat attatttacg ctttcaacaa cagcggcaac
     4861 agatttttct tcgttatacg caggtattac tattgccagt gtcttcatgg ttgtgttgca
     4921 ttaatcatac agcaaaaata gtcagatttg cgttacactg aattattgag aaaaaacata
     4981 agtaataata ttggctccca tttgtaaggc tttcagacgt gcttcatccg agtcgtgatg
     5041 cacactctgg tcttcccaac catcgcccag atcgcattca tacgaataaa aaacaagcag
     5101 tcttccgtcg tgaacaattc caaaaccctg aggagctttg ttgtcgtgtt catgaatctt
     5161 gggcaggcca ttcgggaaat cgaatctgga atgatagata ggatgatcga aaggcagttc
     5221 taccatttcg atatcgggaa atattttttt catttcgcgc cggacatact cgtccatacc
     5281 ataattatcg tcgatatgta agaaacctcc tgaaagcagg tatattctga gattttcagc
     5341 ttcgtaatcc gaaaaaatca cattgccatg cccggtcatg tgaatgaaag cacatgaaaa
     5401 aatgtcagcg cttccgggtt caacattaac atactctgcc tgaatattag tattaaggtt
     5461 ttcgttacaa aatttaatca ggtttggcag gctggtgggg tttgcgtacc agtcgccacc
     5521 gcctttgtat ttaagaagcc ctatccttac actggtggtt tgcgaaaata aagcacaggg
     5581 aaacaggaca ataaaaatga aaacgaattt atacataacc atcaaaaatt atgaatgtaa
     5641 aaatagcaaa tcttgccgta taaatatttt tttgcagaaa ccgaaacttt attatttttg
     5701 taaactctgt tttggagagg tgggtgagtg gctgaaacca ccagtttgct aaactgacgt
     5761 actgagaaat cggtaccggg ggttcgaatc cccccctctc cgctgacaca ctaagcagaa
     5821 cgacgcaaaa gcctgaaaat caatactttc aggttttttg ttttcaccaa aatacgcaaa
     5881 aaaacgcagt tttacacggg ggtttgttac caaatcgtta ccacccattt ttttgaaaaa
     5941 gtggtaacta atcggtgccc ctttgcactg atatgcagca ctttgcattc ttgtttttca
     6001 atcctatgca gtatttttgt ttaacgtaaa aacttatttg aaatggaaaa gaaaaacacc
     6061 ttcacaatgc tcgtgtacat gaaaaaaaca cgcaaagaca aaaatggtaa atcaccgctt
     6121 taccttcgta tcagctacaa ttcgaaccaa gcaaccatca ccttgcacga atcgtgggat
     6181 gaagaattgt ggagcaccaa catgggtatg gccattggta acgacaaatt cgcaaaaaac
     6241 ctgaatcgtt acctaaccga agttaaatca aatgtgtacg atcactttaa acgactgaga
     6301 gacaactcaa aagcattttc tgttgacgat attcgcgatg cttatctagg aattgaaaca
     6361 aaaaaaggac gcactatttt agaagttttt gaagaacata acgagaaagc cagggcttta
     6421 aaaagtattg actttgctcc tgcaaccgtt cagcgatatg aggtttcgaa aatgcatacc
     6481 cgcgatttta tgatatggaa atacaaaaaa gacgacttgc ttttaaccga cattacttgg
     6541 gagtttttgg gagattatga attataccta aaaacggtta gaaattgtgc acacaacacc
     6601 gcgctgaaat acctgaaaaa ctttaaaaag attgtaagaa ttgccatggc caccggagaa
     6661 ctaacatacg atccttttgc caattataag atgaagctga aaccggttga tcggggattt
     6721 ctcactgaag aagaactgga taaaatcatt aaaaaagact tcaaaaatga taggctcgat
     6781 caggtgcgcg actgttttct gttttcatgt tttaccggtc ttgcctatgc cgatattcac
     6841 gacctgattg acgaaaacat tgttatcggt acggatggcg ggaaatggat caaaatcaaa
     6901 cgtaagaaaa cctcaaacct tagcagcatc ccaattttga aaatcacaca aaagattatt
     6961 gataaatata gcgaccaccc cgagtgtatt aagagtaaac gtcttttacc ggtaaagtcg
     7021 aatcagaaaa tgaattgcta tctgcaggag attgccgatg tgtgcaagat tgacaaacac
     7081 cttactagtc acgttgcccg acatactttt gcaacaaccg ttaccctaaa caacaatgtg
     7141 cctatcgaaa ccgtatcaaa aatgcttggt cactcctcta ttgaaatgac tagggtttat
     7201 gcaaggctac ttgataaaaa ggttggccaa gacatgaagc atttggatgg gaagtatgat
     7261 atggctgttt aaaatgaaag ggctgagagg cccttttgtt ttattctaca gttagtttgc
     7321 agcatctgat gcatttcgat ttacaacttt cataatgcag aactacctct caacaataaa
     7381 ctaactgact atttgctaaa cacattgcta tgtggtcgta cttttattaa tttatcaaaa
     7441 aaatcagttc ttgttattaa attcttcctt tcaattattg ctccaagttt ttcaatttta
     7501 accaa
//
LOCUS       DAOOQP010000136         7210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_12747_length_7210_cov_5.233403, whole genome shotgun sequence.
ACCESSION   DAOOQP010000136 DAOOQP010000000
VERSION     DAOOQP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12747_length_7210_cov_5.233403"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLK75_11405"
     CDS             complement(<1..383)
                     /locus_tag="PLK75_11405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39892.1"
                     /translation="MTSVAVIILNWNGASLLRKFLPSVVNYSNMPEVEIWVADNNSAD
                     DSIDVITNEFPEVKYLKFDQNYGFATGYNKAIKHIECKYALLLNSDVEVTPGWLDPLI
                     SMMESDNSIAACMPKIRSYYRRDVY"
     gene            complement(470..1519)
                     /locus_tag="PLK75_11410"
     CDS             complement(470..1519)
                     /locus_tag="PLK75_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOY39893.1"
                     /translation="MRLSLIVVLFLSLLGLVSCTSEFDVNSDWTPIPVVYCVLDQSQA
                     TQYVKVTKAFLGDAPAYVMAQYSDSLFYNDVEVRLYEKNSTGRQLVATFDETNNIPMD
                     TGYFASDRNTLYTSDVTLNPEKEYFLDVQINDIGKTVTNDDPVSLIENCYIATPSVQQ
                     AFINIYSYDNNSSYEYYTGTNGKVYQMIIKFYYLEVIGTDTTENALCIEWPQAVKVDS
                     LSNTNNKVRGEFSVSAWYNLLKESIPTQPNVKRLVRMPVSIEYHLVGADENYYTYMQI
                     SSPSTGLAQSKPLFTNLSGGIGLFAARVNTVVAKKLGERTLDSLSFGMYMYDKGFALH
                     TDPYYQPHFPLPGSK"
     gene            1582..2397
                     /gene="panB"
                     /locus_tag="PLK75_11415"
     CDS             1582..2397
                     /gene="panB"
                     /locus_tag="PLK75_11415"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOY39894.1"
                     /translation="MTDAKEVRKVTTHVLQEMKHQQEKIAMLTAYDYSMGRLLDESGI
                     DVILVGDSASNVMAGYDSTLPITLDQMIYHAASVVRGVQRALVVVDMPFGTYQGNSKL
                     ALQSAIRIMKETGAGAIKIEGGSEVGESIRRILSAGIPVMGHLGLTPQSIHKFGTYIV
                     RATKEEEADILVEDAHLLEELGCFGIVIEKIPARLAGQVAKEVKIPIIGIGAGHDVDG
                     QVLVLHDMLGITQEFSPRFLRRYHNLSKEITDAVKQYIVDVREGEFPNSKEQY"
     gene            2402..3097
                     /locus_tag="PLK75_11420"
     CDS             2402..3097
                     /locus_tag="PLK75_11420"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOY39895.1"
                     /translation="MSNSYQPDILFEDNHLIAVNKRCGEIVQGDKTGDECLADRVKAY
                     IKTRYAKPGDVFLGVVHRLDRPVSGALLFARTSKALERMNSMFREKTIQKTYWAIVKS
                     IPPENEGRLINHLIRNEKLNKSFVVPMGTSNSKESILDYKLLASSDKYHLLEIKLHTG
                     RHHQIRCQLAHIGCPIKGDLKYGFPRSNENAGIDLHSRKIEFIHPVAKTRICVIAPLP
                     ETELWHFFGGLAE"
     gene            complement(3065..3937)
                     /locus_tag="PLK75_11425"
     CDS             complement(3065..3937)
                     /locus_tag="PLK75_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39896.1"
                     /translation="MMNKVIFLVVFVSILSFSCSHKGKNGIQGKNKVKEIELVWAGDS
                     IGDLYIPNSRITMPFTLEGLTEQFYVEFYLDFEGTVLYDEVLRDVSFSNQDLRIKMDS
                     VSAYRQADPMRGQIHGLNGKIGGMGFTNSSFILKKFQGNPGDSVIGRVGLDFFKNKYL
                     IVDLQHPKVLVFDTCPGLMCYEECCFSDFAVNHGRVTVPLHIDDKSYNFWVCPQSKAY
                     VFFSKKNLTRSKVFFANQQVGPPMTYPEKDSFIAYEKIDGILGAPFFSEQKIVIDLIH
                     NKIALFSQPTEEMP"
     gene            4154..6016
                     /locus_tag="PLK75_11430"
     CDS             4154..6016
                     /locus_tag="PLK75_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39897.1"
                     /translation="MGKPNVKKEISKKGSHPKQAVQAKPVSFLAQAIAVVVLLIATIL
                     AYQNGMRGMLLNFDDVEYFFNYPEVVNLSWESVKTYFSGYYVLMYQPLPILSFALNYH
                     FTGLNTFPMHMVNLFFHLSNIILVYFLTKNLSKRFDVSIIVAALFALHPMNIEAVTWI
                     SARSSGMYTFFYLLSLIFYVKYIRSELKWKYIVAAILFFIFSLFSKAQAVTLPVVLLA
                     IDYYMNRKLISWRVVLEKIPFFILSVIFGLVAISEEGTVTNMTQGMMIHYTTFESFFL
                     VCYSFVFYLYKLFVPFSLSAIYVFPPKVNGYLPMIYYLSPVILALLTFLFFRFARKKR
                     YIVFGLAIFLITISLNIQIIPSRLFIVTDRYGYFPYLGLFFIIACFISENRDKKFLNA
                     KMAFVLPALLILYLCAFTITIYRHNKVWDNDIVFMTHLIEKNPEVPYISRAYGNRGHA
                     YFELADYRTAISDFNNAVRIKPDDAKSYFNLGASYLKLKEYEQAISSFDSAAKYEPKQ
                     MIIYENRAIARFNLKDYEGTIRDCRYAISLDSTVTDLFNLRGVSRYYIKDFKGAEEDF
                     SKCIRLNPQYGEAYKNRGYLYQEMQRLSEACSDWNHALEYGFPEVQEAINLYCK"
     gene            6054..6431
                     /gene="crcB"
                     /locus_tag="PLK75_11435"
     CDS             6054..6431
                     /gene="crcB"
                     /locus_tag="PLK75_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOY39898.1"
                     /translation="MLKTILLIGSGSFIGGVSRYFVARAVQNTWITSFPAGTLIVNLS
                     GCLLIGLLYGLFEKYNVLSDDIRFFATVGFCGSFTTFSTFAFENVALLRDGNWINASI
                     YTGLSVFLGIFAVYAGRIIVKTL"
     gene            6434..6781
                     /locus_tag="PLK75_11440"
     CDS             6434..6781
                     /locus_tag="PLK75_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOY39899.1"
                     /translation="MENTGNARLMRIFLSSTDKFRSTPLYEVIIFAAKRYQMSSAIVV
                     KGIMGFNAGSNINTINFWDISERLPLIVEIIDTSEKTEKFLEIIKPYFEKLRNGCIVT
                     TEVVDIAFIKMNR"
     gene            6792..7088
                     /locus_tag="PLK75_11445"
     CDS             6792..7088
                     /locus_tag="PLK75_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOY39900.1"
                     /translation="MKKNNLITEEQKITARYAKALGHPVRVYILSLLAKQSCCYSGDL
                     TEVLPIAKSTLSQHLKELKNAGLIQGEIEAPKIRYCLNRENFMEAKKLLNFLFD"
BASE COUNT         2190 a         1402 c         1442 g         2176 t
ORIGIN      
        1 tcatagacat ctcttctata atagctacgt atcttaggca tacaagcagc aatgctattg
       61 tcagactcca tcatgctaat taacgggtcg agccatccgg gagtaacctc aacatcggag
      121 ttcagcagca atgcatattt gcattcaata tgctttattg ctttattata gccggttgca
      181 aagccgtagt tttggtcaaa cttaagatat tttacttcgg gaaattcgtt tgtaataaca
      241 tctattgaat cgtctgcaga gttgttatca gcaacccaaa tttcaacctc gggcatatta
      301 ctgtaattca caaccgaagg caaaaacttc ctgagaagcg aagcgccgtt ccagttcaga
      361 ataattacag ctactgatgt catgcacgca aaagtattaa atttttaatt tggtgcttgt
      421 atatatgaac gcattataaa aacattaaca gtttgggaaa agttttatgc tatttgctgc
      481 ctggtaacgg gaaatgaggc tgataatacg gatcggtatg aagggcaaaa cctttgtcgt
      541 acatatacat tccaaaacta agcgagtcga gtgtacgttc tcccaatttt tttgcaacaa
      601 cggtgtttac tctggctgca aaaaggccta tgccgcctga aagattggta aataagggtt
      661 tcgactgagc caaccctgtt gacggcgacg aaatctgcat atatgtgtaa tagttttcat
      721 ctgccccaac caaatgatat tcaatactca caggcattct gacaagtcgc ttcacattgg
      781 gttgtgtcgg aatggattct ttcagcagat tataccatgc cgaaaccgag aactctcctc
      841 tcactttgtt atttgtattc gaaaggctgt caacttttac agcctgaggc cattcaatgc
      901 acagtgcatt ttcggtggta tcggtaccta ttacttcgag gtaatagaac ttaattatca
      961 tctgatacac ttttccattt gtaccggtgt agtattcgta gctggaatta ttatcgtagc
     1021 tgtaaatatt aataaaagcc tgctgtaccg aaggtgtggc aatatagcaa ttttctatca
     1081 ggcttacagg atcatcgttg gtaaccgttt ttccgatatc gttaatttgc acatcaagaa
     1141 aatactcttt ctcagggttc agggttacat cagaagtata caatgtattt cggtcactgg
     1201 caaaataccc ggtatccatg gggatgttgt ttgtttcatc aaaggtggca acaagctgcc
     1261 tccctgtgct gtttttttcg taaagcctga cctcaacatc gttataaaac agagaatcgc
     1321 tgtattgagc cataacataa gcaggggcat cacccagaaa agctttagta actttcacat
     1381 actgagttgc ctgcgattga tcaagtacac aatatacaac ggggataggg gtccaatcgg
     1441 aattaacgtc aaattcgctg gtacaggata ccaaacccag cagagaaaga aaaagaacaa
     1501 ctattaagga caaccgcatt caaataagtt ttataaataa acgtcaaaat taattaatat
     1561 tgcattataa aaaataaaaa aatgaccgac gcaaaagaag tacgcaaagt aaccactcat
     1621 gtgttgcagg aaatgaagca tcagcaggag aaaattgcca tgctcactgc ctacgattat
     1681 tctatgggac gcttgctcga tgaatcgggt atcgatgtaa ttctggttgg cgactctgca
     1741 tcgaatgtga tggcaggtta cgattccaca ttgcccatta cgctcgacca gatgatttac
     1801 catgcagcaa gtgttgtaag gggagttcaa cgggctttgg tggttgttga tatgccattt
     1861 ggaacttatc aggggaattc aaagcttgcg cttcaatctg caatcaggat aatgaaagaa
     1921 accggagcag gtgccataaa aattgaaggc ggatcggaag ttggagaatc aatccgacgt
     1981 attctcagcg ccggaatacc ggttatgggt catctggggc ttaccccgca gagcattcac
     2041 aaatttggca cttacattgt tagagcaacc aaagaagagg aagccgatat tcttgttgaa
     2101 gatgctcacc tgcttgaaga actcggttgt tttggtattg taatcgaaaa aatacctgca
     2161 cgccttgccg gacaggtcgc aaaagaagtt aaaattccaa ttatcggtat cggtgccgga
     2221 catgatgttg atggccaggt gttggtactt cacgatatgc ttggtattac acaggagttt
     2281 tcaccacgtt ttttgcgcag gtatcataat ctgagtaaag aaattactga tgctgtgaaa
     2341 caatacattg ttgacgtgcg agagggagag tttccaaaca gcaaagagca atattaaccc
     2401 attgagcaat agctatcaac cggacatttt gtttgaagac aatcacctga ttgctgtaaa
     2461 taagcgctgt ggggaaattg tacagggtga taaaaccggc gacgaatgtc tggccgatcg
     2521 cgtaaaggcg tatattaaaa cccgatatgc caaaccggga gatgtgtttt taggggttgt
     2581 acaccgtttg gacaggccgg ttagcggagc attgctgttt gcccgtacca gtaaagcttt
     2641 agaaagaatg aactcaatgt ttcgcgaaaa aacaattcag aaaacctatt gggcaattgt
     2701 taagtctatt ccgcccgaaa acgaaggcag gctgattaat cacctcatcc ggaacgaaaa
     2761 gctgaataaa tcgtttgtgg tgcccatggg cacttcgaac agcaaagaaa gcattcttga
     2821 ttataagcta ctggcatctt ccgacaaata ccatcttctt gaaattaaac ttcacacggg
     2881 gcgtcatcat caaatacgtt gtcagctggc tcacattggc tgtccaataa aaggggatct
     2941 aaaatatggt tttccccgat caaatgaaaa tgcggggatc gacctgcatt cacggaaaat
     3001 tgaattcata catccggttg caaaaaccag aatttgtgtt attgctcccc tccccgaaac
     3061 tgaattatgg catttcttcg gtgggttggc tgaataatgc tattttgttg tgaattaagt
     3121 caattactat tttctgttcc gaaaaaaatg gtgcacccaa tataccatct attttttcgt
     3181 aggcaataaa agaatccttt tcgggatatg tcataggagg accaacctgt tggtttgcaa
     3241 aaaacacttt ggaacgcgtc aggttctttt ttgaaaagaa cacataagcc tttgattgcg
     3301 gacacaccca gaaattataa gatttatcgt cgatgtgcaa aggcacggta actctgccat
     3361 gattcactgc gaaatcagag aagcagcact cctcgtaaca catcaatcca gggcaggtgt
     3421 caaatactag tactttgggg tgttgcaaat caacaatcag atatttgttt ttaaagaaat
     3481 cgagcccaac cctgccaata acagagtccc ccgggtttcc ctgaaatttt ttcaatataa
     3541 aactactgtt tgtaaatccc ataccaccaa ttttgccatt caatccgtga atctgaccac
     3601 gcattggatc ggcctgtcga taggcagaaa cagagtccat ttttattcgc aaatcctgat
     3661 ttgagaaact gacatcccta agaacttcat cgtaaagaac agttccttcg aaatcgaggt
     3721 aaaactccac ataaaactgt tcggtcagtc cttcaagcgt aaaaggcatt gttatccgcg
     3781 aattcggaat gtacaaatca ccaatactgt cgcctgccca tacaagttca atctctttca
     3841 ccttattttt gccctgtatg ccatttttac ccttatgtga gcatgaaaaa ctcagtatag
     3901 aaacgaaaac aaccagaaat attactttat tcatcattcg aaattgactt gacaagtaaa
     3961 gttactacat tttttaatgt aataaaagaa aagtcagtta tattatcgaa aattatcgga
     4021 aactaattat gcatcgggtt gttaggcaaa ttctcaacat aatgcagaat cggctgtagt
     4081 ttttgtaaaa gttaaaattt tatacctttg aaaacgcaaa tttttaacaa ggggttatta
     4141 aacaaatact tgtatgggga aaccaaatgt taagaaagaa atttcgaaaa agggttctca
     4201 tcctaaacag gcggtccaag ccaaacccgt ttcattttta gcacaagcca ttgctgtagt
     4261 agttttattg attgcaacaa tacttgcata tcagaacgga atgcgcggta tgcttctgaa
     4321 ttttgatgat gtggagtatt tcttcaacta tcccgaagta gtaaatctat cgtgggaaag
     4381 tgtaaagacc tacttttcgg gatattatgt tctgatgtat caacctttgc ccatactcag
     4441 ttttgcgctt aattaccatt ttacaggatt gaacactttc cccatgcata tggtgaatct
     4501 gtttttccat ttaagcaata ttattttagt gtattttctt actaaaaacc tatcaaaaag
     4561 gttcgatgtt tccatcattg ttgcagccct ttttgccctg catccgatga atattgaagc
     4621 agtaacatgg atatcggcac gcagcagcgg gatgtatact ttcttttatc ttctctctct
     4681 tatattttat gtaaaatata ttcgtagcga actcaaatgg aagtacatcg ttgcagcaat
     4741 tctatttttc attttttcgc tcttttctaa agctcaggca gtaaccttgc ctgttgtact
     4801 gcttgccatt gattattaca tgaaccgtaa gcttatttcg tggcgtgtgg ttcttgaaaa
     4861 aataccgttt ttcattctgt cggtaatatt tgggcttgtc gcaataagcg aagaaggcac
     4921 cgttaccaat atgacccagg gaatgatgat acattacacc acttttgaat cgttctttct
     4981 ggtgtgctat tcattcgttt tttacctgta taagcttttt gtacctttct ctctatcagc
     5041 aatttatgta tttcctccaa aagtgaatgg ctatcttccg atgatttact atttatcacc
     5101 ggtaattctt gcactactta cttttctgtt ttttagattt gcacgcaaaa aacgatatat
     5161 tgtattcgga ctggcaatat tcctgatcac tatttcgctg aacattcaga ttattccctc
     5221 ccgtctattt attgtgaccg acagatatgg ctatttccct tacctgggtt tgttttttat
     5281 cattgcctgt tttatttcgg aaaaccgtga caaaaagttc ctaaacgcta agatggcgtt
     5341 tgttctacca gcattactga tattgtattt atgcgctttt acaatcacta tttaccggca
     5401 caataaggta tgggataacg atattgtttt tatgacgcat ttaatcgaaa agaatccaga
     5461 agtcccctac atatcaagag catacggcaa tcgtggacat gcctactttg aactagccga
     5521 ttacaggacg gctatcagcg attttaataa tgctgtgcgc attaaacccg atgatgccaa
     5581 atcgtatttt aatctgggag ccagttattt aaaactgaag gaatatgaac aagcaatctc
     5641 cagtttcgac agcgcagcaa aatacgaacc caaacagatg attatctatg agaaccgtgc
     5701 catagcccgc ttcaacctca aagattacga aggcaccatt cgcgattgcc ggtatgcaat
     5761 aagtctcgac tccacagtaa ccgacctgtt caacctgagg ggtgtatcaa ggtattatat
     5821 aaaagatttc aaaggtgccg aagaagattt ttcgaaatgt attcgtctga atccgcaata
     5881 tggagaggca tacaaaaatc gtggatatct ctatcaggaa atgcagcggc tcagtgaagc
     5941 ctgcagcgac tggaaccatg ctctggagta tgggtttccg gaagtacagg aggcaataaa
     6001 tttgtattgt aaataattac aggttagtga aatgaccatt tgaaaagttt tacatgttaa
     6061 aaacaattct attgattggc agcggaagtt ttattggagg ggtaagccgg tatttcgtag
     6121 cacgcgctgt tcaaaatact tggatcacat cttttccggc aggaacactg attgtaaatc
     6181 tgtcgggctg cctgctgatt ggattattgt atgggctatt cgaaaaatac aatgtacttt
     6241 cagatgatat caggtttttt gcaacggttg gtttttgcgg cagttttacc acattttcaa
     6301 cttttgcttt tgagaatgta gctttgctcc gcgacggaaa ttggattaat gcttcgatat
     6361 ataccggatt gagtgtattt ttaggtatat tcgcagtata tgctggcaga ataattgtta
     6421 aaactttata agcatggaaa ataccggaaa tgcccgattg atgcggatat ttttaagcag
     6481 caccgacaaa ttcagaagca ctcctttata tgaagtgata attttcgctg caaaacgcta
     6541 ccaaatgtca agtgccatag ttgtgaaagg cattatgggt tttaatgccg gtagtaacat
     6601 taataccatt aatttctggg atatctccga acgacttccg ctcattgttg aaattattga
     6661 cacaagcgaa aaaaccgaaa aattcctcga aattataaaa ccttatttcg agaagttacg
     6721 caacggttgc attgttacaa ctgaagtcgt ggatattgca tttattaaaa tgaatcgctg
     6781 aattcaatat tatgaaaaaa aacaatctga taaccgaaga acaaaagatc actgcccgat
     6841 acgccaaagc cttaggtcat ccggtgaggg tatatattct cagcctgctt gcgaagcaat
     6901 cgtgctgcta cagcggtgac ctaactgagg tgctgccaat tgcaaaatct accctatcgc
     6961 aacacctgaa agaattgaaa aatgccggtt taattcaagg cgagatcgaa gcgcccaaaa
     7021 taaggtactg cctgaatcgt gaaaatttta tggaagcaaa aaaattgttg aactttttgt
     7081 ttgattaatt ttttttaata attatgttcg taattttacg aacttcgaat caaacattaa
     7141 attttatatg atgaaagatt agtagtggtg cgttaatttt ttaatcaaat agtgtaggtt
     7201 gttctttgac
//
LOCUS       DAOOQP010000137         7181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_12810_length_7181_cov_4.838759, whole genome shotgun sequence.
ACCESSION   DAOOQP010000137 DAOOQP010000000
VERSION     DAOOQP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_7181_cov_4.838759"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLK75_11450"
     CDS             <1..316
                     /locus_tag="PLK75_11450"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOY39901.1"
                     /translation="IYLKENINLGDYKELYDQKFRELKDSTVFYFRNDYTYERVAKGQ
                     STKGTWSISNDGTLVIVKMIGKEAEEKSRILELTKTNLVMSPMSESSNSKVVMHKVID
                     NE"
     gene            309..752
                     /locus_tag="PLK75_11455"
     CDS             309..752
                     /locus_tag="PLK75_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39902.1"
                     /translation="MNDIAERRYIVAVDFDGTIVEHKYPSIGKEMPLAFEVLREMQEA
                     GYRLILWTFRDGEYLKDAVEYCLSNGIMFYAVNQSSPNEEFNKYMSRKINADIYIDDR
                     NVGGFPGWEKIREWLLPDSPNINRNNNVKRNNDNKKRGFFRDLFI"
     gene            901..4194
                     /locus_tag="PLK75_11460"
     CDS             901..4194
                     /locus_tag="PLK75_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY39903.1"
                     /translation="MKKSLFIFSVLFLMVAGLFAQEGIYLKPNASGAIITENTDTRLV
                     FQSDISIIPLQEVKTKDGIYSRIYLPGYTPNTDYGMPELPNLNRIIEVPYGAKIAVNI
                     VAYNEQVISLDEYGFQYQLMPCQPSVFKNQDPSQLVFYKDENTYNGVSAYSPETVSVT
                     LLSKMRGVQLARLSINPFTYDAQSNTLIVKNDIVVEIRFENADLTAYNETKARYYSPS
                     FGNLYAGVWNYKAPATKDALSQYPIKYVIVSSPMFEEALQPFIEWKTKKGFTVIEAYT
                     NNPSVGTTVTSIHNYLKGLYDAATIEDPAPTYVLLVGDLAQIPATNAGQHYSDMYTVE
                     FDGGSDYVPDMYIGRFSATNVTQLQPQIDKTLMFEEYTFPDPAFLDKVVLVAGADNNY
                     GPTHANGQINYANQYYFNSAHGMDPYVYLYPASANSETAILGNLSSGVGFVNYTAHCG
                     SDGWGDPSFTTSNIPSLQNDNEYFFSIGNCCLSNKFDESECFGEALLRAQHKGAVVHI
                     GGSNSTLWNEDFYWSVGVTATINANPTYSGTTQAAYDHLFHENGEDPYVDAAQITYMG
                     NMSVMQSSSSEDEYYWEIYHVMGDPSLMPYAGVPSELEADYFASVPIGVSQLLVSTEP
                     GAYVAISLDGVLLDARLADENGEALLQFEAFSTVCNPDVVATKQFRSPYIGTLQVIPN
                     DNDYDAMLKSIVLPTNIMNIADATFQPTFEIMNLGQLNLTSVTVSYQIDEESPVQTAW
                     TGDLDFLGVTTVLFDEITLAEGQHTITASVSMPNGNTDQYPSNDQMTKNFLVYSGNVS
                     LLTVESPIEIYCNENSFVPEITIKNLDPTPLTSLTCAYTVGLINYEFVWTGNVAQNQT
                     TNIQFPEASFASGNHTIEFTISEPNGGSDMNDADNYAEKDFRINSPGQIVHLTLLTDN
                     YGTETTWELSDNESQAVLYSAGPYTNSVATFNYDWCLGEGCYTFTILDSYGDGMSGSS
                     WFGIPPGHVTITNTVTSEVYFDLAGDDFEDSAEDSFCITIDNSNAISKTNATIVFPNP
                     ASDFVNVIASEPIIGLTVINSLGQSTFCSSNESDNIVLDTKDYVAGIYILKVETVSGI
                     SIHKLQINK"
     gene            complement(4267..4746)
                     /locus_tag="PLK75_11465"
     CDS             complement(4267..4746)
                     /locus_tag="PLK75_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39904.1"
                     /translation="MKHFIICVLSSLLCIANSKAQDNEYNSIGKNGIFTEVYILRHDF
                     NSGFISLNYERFLGHKKRISLRGGFYPDFKTLYTFPFTCTWVTHPLKKHHIEFGLGFV
                     YRIEIYNENVYHDIPALMLPIMYRYQPAKGFYFRGGINLFYSWPVLPSPSISAGYRF"
     gene            complement(4908..>7181)
                     /locus_tag="PLK75_11470"
     CDS             complement(4908..>7181)
                     /locus_tag="PLK75_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HOY39905.1"
                     /translation="SYVVNGGEFRNCILWGNTDQNGLSNYYGSNYIYCNTTPLPSGAG
                     NISTDPGFVDSGNNDYRLVDISPSVNTGTNGYNAQTYDVRGQARIQGGTIDMGAYEWT
                     TGIDPVATTPEITCPADIYVNNDAGLCTAVVNYDAAVATGTPPPSLIYSHASGSEFPV
                     GTTTVTVTATNIVGVNQCTFEIIVSDAEIPTITCPPNLAINTDTDLCTASGVVLGISI
                     TSDNCGVLSTLSDAVEPYAVGTHIITWTVTDASNNSATCQQTVTVTDAQVPTIACPSN
                     VSVNTDADFCTASGVDLGTPTAGDNCSFTVTNDGVEPYAVGTHIITWTVIDASNNSAT
                     CEQTLTVTDNQLPVINCPADVLVNTDTDLCTASGVDLGTPTTSDNCDILSVTNDAVEP
                     YSVGVHTITWTVEDVNGNTETCEQTLTVADAQLPVITCPEAVEVNADADMCTASGVEI
                     GDAVTGDNCGVLSVTNDAAEPYAVGVNIVTWTVEDVNGNTNTCQQSVTVIEEELPEIT
                     CPANVSVNTDADLCTAGGIVLDEPITSDNCGIYSVVNDAPETFETGETTVIWTVTDNN
                     GNIATCSQVVTVTDNQAPAIVCPPNVSVPFDEGENTASNVDLGTPETSDNCGIQTVEN
                     DAVEPYPVGITTVTWTVTDVNGNTATCTQTVTVYDPIYVVQFTEDGKNYISNYPNPFT
                     NATTIEFGILHNSKVSITIVDLNGRKIASLFDGVVFEKGVHHFNFNSNFYNLCPGEYI
                     IQLQTGNKMVTNKMAVK"
BASE COUNT         2127 a         1431 c         1502 g         2121 t
ORIGIN      
        1 catctatttg aaagaaaaca ttaatctggg cgattataag gaattatacg accagaagtt
       61 tcgggaactg aaagacagta ctgtttttta ttttcggaac gactatacgt acgaaagagt
      121 tgctaaggga caatcaacga aaggtacctg gagcataagt aacgacggga cattggttat
      181 tgttaaaatg ataggaaaag aagcggaaga aaaaagcaga atacttgaac ttacaaaaac
      241 caatctggtg atgagtccta tgagcgaaag ctccaactca aaggttgtaa tgcataaagt
      301 gattgataat gaatgatatt gctgagagaa gatacattgt ggctgttgat ttcgacggga
      361 caattgttga acacaaatac ccttctatag gaaaagaaat gcctcttgcg tttgaggtgc
      421 tacgtgaaat gcaggaagcc ggatatcgtt tgattctatg gactttcaga gatggtgaat
      481 atctgaaaga tgcagtcgaa tattgtctga gcaatggtat aatgttttat gctgttaacc
      541 aaagctcgcc aaacgaggaa tttaataaat atatgagccg gaaaattaat gctgatattt
      601 atatcgacga taggaatgtg ggtggttttc cggggtggga aaaaattcga gagtggcttt
      661 tacccgattc accgaatatc aacagaaaca acaatgtgaa aaggaacaat gacaataaaa
      721 aacgcgggtt tttccgcgac cttttcattt aagactgggt tgatatttct acattgttaa
      781 catttgttaa aaaacaaaaa taattttcag aacctccttc gaaaattatc taaatccatg
      841 ttcatataag tattatcttt gaacccataa agatttatta aaattaaaaa cagaataata
      901 atgaaaaaat ccctttttat ctttagcgta ttatttttaa tggtggctgg tctgtttgcg
      961 caggaaggca tttatttgaa accaaacgca tccggtgcaa ttataaccga aaacaccgat
     1021 acacggcttg tttttcaaag tgatataagc attattccgt tgcaggaagt taaaaccaaa
     1081 gacggaatat attcaagaat ttatctaccc ggatatactc caaacaccga ttatgggatg
     1141 cccgagcttc caaatttgaa ccgaattatt gaagttcctt acggagccaa aattgctgtc
     1201 aatattgttg cttacaatga acaggtcata tctcttgacg aatacgggtt tcaatatcaa
     1261 ttgatgccat gtcagccttc ggttttcaag aatcaggacc cctcgcaatt ggttttttat
     1321 aaagacgaaa acacatataa tggagtatct gcgtattcac ctgaaacagt ttcggttacg
     1381 ttgctaagta aaatgagagg cgttcagtta gcccggctta gcattaaccc atttacctac
     1441 gacgcacagt cgaacacgct tattgtgaaa aacgatattg tagttgaaat acgcttcgag
     1501 aatgctgact tgacagctta taacgaaaca aaggccagat attattcccc atcgttcgga
     1561 aatctgtatg ctggcgtatg gaattacaaa gctcctgcaa ccaaggatgc tttatcgcaa
     1621 tacccgatta aatatgtaat tgtatcgagc cctatgttcg aagaggcact tcaaccgttt
     1681 attgaatgga aaaccaaaaa gggttttact gttattgaag cctataccaa taacccttct
     1741 gttggcacca ctgttaccag cattcacaat tacctcaaag gtttgtatga tgcagctaca
     1801 atcgaggacc ctgctccaac ttatgtgctt cttgtaggtg atttggcaca aattccggct
     1861 actaatgcag gccaacatta cagcgatatg tatacagttg aatttgatgg gggtagcgac
     1921 tatgttcccg acatgtacat tgggcgcttt tcggcaacca atgtaactca acttcagccc
     1981 cagattgata agacacttat gtttgaagag tatactttcc ccgatccggc tttccttgat
     2041 aaggttgttc tcgttgccgg cgcagataat aattatggtc ctacccacgc caatggccag
     2101 ataaattatg ctaatcaata ctattttaac agtgcgcatg gtatggatcc ttatgtatat
     2161 ctgtacccag cgtctgcaaa ctccgaaact gctattttag gaaatctgag tagtggcgtt
     2221 ggatttgtta actatacagc acattgtggt tccgacggat ggggtgaccc ttcgtttaca
     2281 acttcgaata ttcccagcct gcagaacgat aacgaatatt ttttcagtat tgggaattgc
     2341 tgtctgtcga acaaatttga tgaatcggag tgttttgggg aagcattgct gagggctcag
     2401 cataaaggcg cggttgtgca catcggagga tcgaacagca ctctgtggaa cgaagacttt
     2461 tactggagcg taggtgttac tgcaaccatt aacgcaaatc caacctattc cggtactacc
     2521 caggcagctt acgatcacct gtttcatgag aatggcgaag acccctacgt tgatgctgct
     2581 caaattacct atatgggtaa tatgtcagta atgcagagca gctcctcgga agacgaatac
     2641 tattgggaaa tatatcacgt gatgggagac ccttctctaa tgccctatgc aggagtaccc
     2701 agcgagcttg aagccgatta ttttgcatca gttccgattg gtgtttcgca attgctggtt
     2761 tccacagaac ccggtgctta tgtagcaatc agccttgatg gagttctcct tgatgcccga
     2821 cttgctgatg aaaatggtga agccctgttg caatttgaag cttttagtac cgtctgcaac
     2881 cctgatgtag ttgctacaaa gcaattccgt tccccatata ttggaaccct gcaggtgatt
     2941 ccaaacgaca atgattatga tgcaatgctc aaatcaattg ttttaccaac aaatatcatg
     3001 aacattgccg atgctacttt tcaacctaca ttcgaaataa tgaatttggg tcagttgaat
     3061 ttaacgtctg ttactgtaag ctatcagatt gatgaagaaa gtccagttca gacagcttgg
     3121 acaggagatc ttgatttcct aggtgttaca acagttttgt tcgatgaaat cactcttgcc
     3181 gaaggacagc acacaattac agcatcggtg agtatgccca atggcaatac cgatcaatac
     3241 ccttctaacg accagatgac gaagaatttt ctggtttatt caggtaatgt aagtttgttg
     3301 acggttgaat caccaataga aatttactgc aacgaaaact ctttcgtacc cgaaattacg
     3361 attaagaatc ttgaccctac tccgcttacg agtttaacct gtgcttatac tgttggctta
     3421 ataaattatg aatttgtatg gacaggcaat gttgcacaaa accaaacaac caatattcag
     3481 tttccggagg caagctttgc ttcgggaaat catactatag agtttaccat ttcggaaccc
     3541 aatggtggta gcgacatgaa cgatgccgat aattacgccg aaaaagattt caggattaat
     3601 tcccccggcc agattgttca tcttacattg cttaccgata attatggaac cgaaacaaca
     3661 tgggaacttt ctgataacga aagtcaggca gtgctatatt ctgctggacc atatacaaat
     3721 agtgtagcca cttttaacta cgactggtgc ttaggcgaag gttgttacac ctttaccata
     3781 ttagattcat atggtgatgg tatgagtgga tcttcttggt ttggaatacc tcccggtcat
     3841 gttaccatta cgaacacagt taccagcgaa gtatatttcg atcttgcagg agatgatttt
     3901 gaagacagtg cagaagacag tttctgcatt acaatagata attcaaacgc aattagtaaa
     3961 accaatgcta ctatagtttt cccaaaccct gcatctgatt ttgttaatgt aattgcttcg
     4021 gaaccgatta ttggcttaac ggttatcaat agtctcggtc aaagtacatt ttgctcttcg
     4081 aatgaatcag ataacattgt tctcgataca aaggactatg tcgcaggcat ttacatttta
     4141 aaagttgaaa cggtttcggg aatatcaatt cacaaattgc aaatcaataa ataacaaaag
     4201 tcattatagc attatcaaaa gccgcaattc atgttgcggc ttttttttga tttatgtaag
     4261 tgtgtattaa aacctgtaac cagcactaat tgaaggggaa ggcagcaccg gccaactata
     4321 aaataaattg attccgcctc tgaaataaaa cccttttgca ggctgatacc gatacattat
     4381 tggtagcatt agggctggta tgtcatgata gacattctcg ttgtatattt caatccgata
     4441 aacaaaccct aacccaaact ctatatggtg tttttttaaa ggatgagtaa cccaagtgca
     4501 tgtaaacggg aatgtgtaca gtgttttgaa atcaggataa aagcctccac gcagtgatat
     4561 acgtttttta tgacccagaa aacgttcata gttcaacgag ataaatccag agttgaagtc
     4621 atgccttaaa atatacactt ctgtgaatat accatttttc ccaatagaat tgtactcatt
     4681 gtcctgtgct ttcgaatttg ctatgcagag caaagatgat aagacacaaa taataaaatg
     4741 cttcatagat tcccccggta taaaataatc ctaatttgaa gaagttaatg ctctcgtatg
     4801 ttttttaata tcaacatgta aaggtattat ttgtttttca ataatttggc aaatggttta
     4861 ttcctaatag gcagaagtag gaattgcatt tttgtcagtc gaaaaaacta tttaaccgcc
     4921 attttattcg taaccatttt atttccggtt tgcagttgaa ttatatattc acccggacac
     4981 aggttgtaga aattggagtt gaagttaaag tgatgcactc ctttttcaaa gacaacgcca
     5041 tcgaataagc ttgcaatttt tcttccattg agatcaacaa tggtaatgct gactttcgag
     5101 ttatgcaaga ttccgaattc aatcgttgtt gcgtttgtaa acgggttcgg ataattgctg
     5161 atatagtttt taccgtcttc ggtaaattgt acaacataaa tggggtcgta aacggtaaca
     5221 gtctgagtgc aggttgccgt atttccgtta acatcggtaa cggtccatgt tacagtagta
     5281 attccgacag gataaggttc aacggcgtcg ttttctacag tttgtattcc gcaattatcg
     5341 cttgtttcgg gagtacccaa atcgacattt gaggcagtgt tttcgccctc gtcaaaagga
     5401 acgctaacat ttggcgggca aacaatagcg ggagcctgat tatcggtaac agtaaccacc
     5461 tgagagcagg ttgcgatgtt cccgttattg tcggtaaccg tccaaataac ggtagtttca
     5521 ccggtttcaa aagtttcagg agcatcattg acaaccgaat aaattccgca gttatcgctg
     5581 gtaattggct catcgagaac aataccgccg gcagtacaga ggtcggcatc ggtattaacc
     5641 gaaacatttg ccgggcaagt tatttcaggc agctcttctt caattaccgt aaccgattgc
     5701 tggcatgtat tggtatttcc gttgacgtct tccacggtcc aggtaacaat gttaacgccg
     5761 acagcatacg gttcggcagc atcgtttgta acgctgagta caccgcaatt gtcgccggtt
     5821 acagcatccc caatttcaac tccactggca gtacacatat cggcatcagc gttaacttcc
     5881 actgcttccg gacaagtaat taccgggagt tgggcatcgg caaccgtaag ggtctgctca
     5941 caggtttctg tatttccatt cacatcttct actgtccatg taattgtatg aactccaacg
     6001 ctatatggtt caaccgcatc gttggtaacc gaaagtatgt cgcaattatc ggaagttgta
     6061 ggtgtaccca aatccacccc gcttgcggta cataaatcgg tatcggtgtt caccaataca
     6121 tcggcagggc agttaattac cggcaattga ttgtcggtaa ctgtaagggt ttgctcacag
     6181 gtagccgaat tgttgctggc gtcgataact gtccatgtga taatgtgtgt tccaactgca
     6241 tacggttcaa ccccgtcgtt ggtcacagtg aagctgcagt tatcgccggc tgtaggtgtt
     6301 cccaaatcaa ccccacttgc tgtacaaaag tctgcatcgg tattaaccga aacatttgat
     6361 ggacaagcaa ttgttggaac ctgagcatcg gtcacggtaa ctgtttgctg acaggtagcc
     6421 gaattgttgc tggcatcggt aaccgtccat gtgataatgt gtgttccaac tgcatatggc
     6481 tcaactgcat cgcttaaggt ggatagaacc ccgcagttat cgcttgtgat tgaaattcca
     6541 agtacaacac cgctggcagt acaaaggtct gtgtctgtat ttatagcgag gtttggagga
     6601 catgtaattg taggaatttc agcatcactt acgattattt cgaatgtaca ttgattaact
     6661 cctacaatgt tggtagcagt aactgtgact gttgttgttc caacagggaa ttccgagcct
     6721 gatgcatggg aatagataag tgaaggagga ggggtaccgg tagcaacagc agcatcgtaa
     6781 ttcacaacag cagtgcataa tccggcatcg ttatttacgt aaatatctgc agggcaggtt
     6841 atttcaggtg ttgttgctac aggatcaata cctgttgtcc attcgtatgc tcccatatca
     6901 attgttcccc cttgaattct ggcttgtccg cgtacatcat acgtctgtgc attatagcca
     6961 ttggtacctg tgtttaccga gggtgaaata tcaaccaacc tataatcatt gtttccagaa
     7021 tcaacaaaac cgggatctgt tgaaatattt ccggctcctg aaggtagtgg ggttgtattg
     7081 caataaatgt aattacttcc ataatagttc gatagtccat tttggtcggt atttccccaa
     7141 agaatacagt tgcgaaattc ccctccattt acaacgtagc t
//
LOCUS       DAOOQP010000138         7092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_13021_length_7092_cov_5.353133, whole genome shotgun sequence.
ACCESSION   DAOOQP010000138 DAOOQP010000000
VERSION     DAOOQP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13021_length_7092_cov_5.353133"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..813
                     /locus_tag="PLK75_11475"
     CDS             103..813
                     /locus_tag="PLK75_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOY39906.1"
                     /translation="MSAEDQLIHEALTDRTWSEIKSNDSWSIFKMMGELVSGFEHLAK
                     IGPCVTIFGSARTQPSNKYFQMAADTAFMLTKKGFGIITGGGPGIMEAANKGANAGGG
                     KSVGCNIKLEFEQYPNKYIDNDKLVTFDYFFIRKVMFMKYSQGFIVFPGGFGTFDELF
                     EAMTLIQTKKVARFPIILVGSDYWNGLLVWVRDIMLQKENNISESDLDLIHVVDTAED
                     AARIIDEFYNKYKLKPNF"
     gene            complement(797..1600)
                     /gene="asd"
                     /locus_tag="PLK75_11480"
     CDS             complement(797..1600)
                     /gene="asd"
                     /locus_tag="PLK75_11480"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOY39907.1"
                     /translation="MRRKKSLRKKISLLVGKITMWRKPYWLVRFMIRIFVRFYHIDMS
                     DYNFTIKRGVSFHDFFTRRLKPGARHVAGAIVSPVDAKIMSFGMAGKTTTIRAKGLDF
                     VFSELIDCDHHYQDEVSYTVLYLSPADYHRIHAPFDMQIDEVRHIPGNLFSVKPKSVQ
                     KREKLYSINERVLLTGTSAYGRFWIVLIGAFLVGSVKLNFSSVTTNQRYRKNKTHTAH
                     VSFNAGDEIGHFRFGSTVVMCIHGDLFSKITYQKGERVLMGEELVKSLV"
     gene            complement(1600..2163)
                     /gene="pth"
                     /locus_tag="PLK75_11485"
     CDS             complement(1600..2163)
                     /gene="pth"
                     /locus_tag="PLK75_11485"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOY39908.1"
                     /translation="MKFLVTGLGNIGAEYADTRHNIGFTTLDFWAKDAGVTFEDKRYG
                     MVCEIKYKGRIFVLLKPSTYVNRSGLAVNYYLKKEKLTAESLFVIVDDIALAFGTIRI
                     KPKGSSGGHNGLQNIVDVLATDSFARMRFGIGGNFHQGGQVDYVLGKWMPEELDVLPD
                     KIARMVEAAKSFGTIGLERTMNIFNTK"
     gene            complement(2189..2761)
                     /locus_tag="PLK75_11490"
     CDS             complement(2189..2761)
                     /locus_tag="PLK75_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOY39909.1"
                     /translation="MQHFEIKGEKRTDLGKNASRKLRKLDSVPCVLYGCGENVNFSAT
                     RAELRNLIYSHNVYIVDIKIGKKKYQAIMKDIQYDPISSKVNHIDFLELSDDKPVAIH
                     VPLIINGQSIGVKAGGKLKKNQRLMHIKALPSYLPDIVEVDITDLNLNDSIRVKDVVI
                     ENVELLDQKNKVLVTIVPVRGVETAAPAAE"
     gene            complement(2921..3859)
                     /locus_tag="PLK75_11495"
     CDS             complement(2921..3859)
                     /locus_tag="PLK75_11495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOY39910.1"
                     /translation="MESPIKFFTGQASRYLSEEIVSFYDPEMELGKSTLIRFSDGEFQ
                     PSLNETVRGCTVFIIQSTCPPAENLIELMLMVDAAKRASAYKVVAVIPYFGFARQDRK
                     DKPRVSIGAKVIADVLSASGVDRVMTMDLHADQIQGFFNVPVDHIFASALFVPYIKSL
                     DLSDLVIASPDMGGSKRAKAYSHFLNCGMAIAHKSREEENVVAEMKVIGEVEGKNVII
                     VDDMIDTAGTVCMASDLMLEKGAKSVRVVATHPVLSGPAYERIEKSSISEVVVTNSIP
                     VKPLSDKITVLSVASLFADVIRKVYTHKSISTTFIV"
     gene            complement(4127..5062)
                     /locus_tag="PLK75_11500"
     CDS             complement(4127..5062)
                     /locus_tag="PLK75_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOY39911.1"
                     /translation="MKKESFLLSLGFLMIIGPSVCQNCNTKVLDSYPDGNAKTKIEYL
                     NPNDTSDIKLIGFYNTGDTNFVFSIFHNQRNGVSVYYYDNGHMKYRVNYKNNQLYGES
                     IFWYPSGQLWQQAMYKNGQLTDTLHDYYENGALKSIGVFENGKGETIYYHPNGMLWKT
                     GKILNDQEEGIWVYYYLNGQKLSEGNYKNGLRHGDYKMFYATGELMESGTYNSNVITQ
                     NTFYLKNGQINSNLTDFAKNNRNVVPWTEEQKQTLTSKCLNDNLKENKNRADDYCYCV
                     VEKIELFLSYVEYSALTEKENAELMQKIDTLCDYE"
     gene            complement(5136..5855)
                     /locus_tag="PLK75_11505"
     CDS             complement(5136..5855)
                     /locus_tag="PLK75_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOY39912.1"
                     /translation="MKASCPILITVINLCFLALTACETFEPNDRHNYEQLRLPPVDTL
                     KFTTDEKALITAGDSADRMEIMYYNQQPDSIILRTISTDIRLTEQELALLTDRMYATV
                     RNPQNPGVGIAAPQVGINRRVVWVKRYDKVGKPWEVFINIRITALSDTLKLRNDGCLS
                     IPGVSGQSWRAIWCDVEYDLPDGTHCTERISHEYTAHIFQHEIDHLDAILWIDRRPSS
                     KNVIIEGNPNEYDGEIMPSID"
     gene            complement(5920..>7092)
                     /locus_tag="PLK75_11510"
     CDS             complement(5920..>7092)
                     /locus_tag="PLK75_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOY39913.1"
                     /translation="TYTVTDANGCSAYDSIYVSLGEYPEVNLGPDQEYCDNDIPVIDA
                     GSGFVSYLWSDASSGATISPETSGTYSVTVTNQTGCTAADTISVTVRPAPEVSLGADV
                     QTCANNEVSLSPPSGFTIYEWSTGASMPILNVTESGEYSLTVHDEYGCTAVDTVEVNF
                     FEVETPFLGFDFVSCDSAPLLLDPDGYFKSYLWQNGSDDTVQLAVYPGVYSLTVTDFN
                     GCTSSDNITISHADSPVLTEEFSASGRIVVNAAGGTPPYSYSVDGLYWSPVNSFNQLL
                     EDWYNVYVSDSNSCKDTIRVYVDNNIIIPEFFTPNGDGYNDTWEIIGIFRYPNADITI
                     FDRFGKQVAKYKGNQQGWNGIYARQPVAADTYWYIIMLNSDMGESAPFKGPVTIIR"
BASE COUNT         2103 a         1496 c         1278 g         2215 t
ORIGIN      
        1 tcttattttt tcatgggggc ttaaatctgt acccactaaa atggtagtag aaccactttt
       61 tcaattaaaa ttttagtatt tttatcatta aataattata atatgtcagc cgaagatcaa
      121 ttaattcatg aagcacttac cgacagaact tggagtgaga taaaatcaaa tgactcatgg
      181 tcgattttta aaatgatggg tgagctagtc agcgggtttg aacatcttgc aaaaataggg
      241 ccttgtgtca ctatatttgg atctgcacgc acacagcctt ccaacaagta ttttcagatg
      301 gctgccgaca cagctttcat gcttactaaa aaaggttttg gaataattac agggggaggg
      361 cctggaataa tggaagctgc caataaagga gctaatgccg gtggtgggaa atcggtaggg
      421 tgcaatatta aactggagtt tgaacaatat ccgaataaat atatcgacaa cgacaaattg
      481 gttactttcg attatttctt cattcgaaag gtaatgttta tgaaatactc gcagggattc
      541 atcgtatttc cggggggatt tggcacattc gacgagcttt ttgaggcgat gacactaatc
      601 cagacaaaaa aagtagcacg attccccatt attttagttg gctccgatta ttggaacggt
      661 ttgctggttt gggttcgaga tattatgctg cagaaagaga ataacatttc ggagtcggat
      721 ctcgatttaa tacatgttgt ggataccgca gaagacgcag ccaggattat tgacgaattc
      781 tacaataaat acaaactcaa accaaacttt taaccagctc ttctcccatt aaaacccttt
      841 cacctttctg gtaggtgatt ttactaaaaa ggtcaccatg tatacacata accaccgtgg
      901 aaccaaaacg gaaatggcca atttcgtcgc ctgcattgaa cgaaacgtgt gcagtgtgcg
      961 ttttattttt tcggtacctt tgattggttg ttaccgatga aaaattcagt tttaccgaac
     1021 cgacaagaaa tgctcctatt agaacaatcc aaaatctacc gtatgctgaa gttccagtaa
     1081 gcaataccct ctcgttgata ctgtatagtt tttcccgttt ttgaacggat ttaggtttta
     1141 ccgaaaacaa attgccgggt atgtgcctca cttcgtcaat ctgcatatcg aatggtgcgt
     1201 gaatgcggtg atagtcagcg ggagaaagat acaaaacggt ataactcact tcatcctggt
     1261 aatggtgatc acagtcaatt aactctgaaa aaacaaaatc gagtcctttt gccctgattg
     1321 tagtagtttt tccggccata ccaaagctca taattttagc atcaacaggc gacacaattg
     1381 cccctgcaac atgccttgcc cctggtttca aacgtcttgt aaaaaaatca tgaaagctta
     1441 cacctctttt aattgtgaaa ttataatcac tcatatcaat atggtagaac ctcacaaaaa
     1501 ttcgtatcat aaatctgacg agccagtaag gttttcgcca catggtaatt ttcccaacca
     1561 gcaatgatat ttttttgcgt aagctctttt tccggcgcat tatttcgtgt tgaaaatatt
     1621 cattgttctt tcaaggccaa tagtaccaaa acttttcgca gcttcaacca ttcgtgcaat
     1681 tttatccggt agaacatcta attcctcagg catccatttc ccaagcacat aatcgacctg
     1741 cccgccctga tggaaatttc cgccaatccc aaagcgcata cgtgcaaagg aatcggttgc
     1801 caatacatca acaatattct gcaaaccgtt gtgtcctccg gaactacctt tgggttttat
     1861 ccgtatcgtt ccaaaagcta aggctatatc gtccacaata acaaacaggc tttcagcggt
     1921 gagtttttcc tttttcaaat agtaattaac tgccaaacca cttctgttta cataggtact
     1981 gggcttgaga agaacaaata ttctgccctt gtactttatt tcgcatacca tgccatatcg
     2041 tttatcctcg aaagtgacac ctgcatcttt tgcccaaaaa tctaaggttg taaaacctat
     2101 attgtgtcgg gtatccgcgt attcagctcc aatgttacct aatccggtta ccagaaattt
     2161 cattattcca atgagtacgt aaaactcatt attcagctgc cggagcagcg gtttcaacgc
     2221 ctcttacagg aacaatagta acgagaactt tatttttctg atcgagaagt tcaacatttt
     2281 caatcaccac atctttaact ctgatagaat cgttcagatt caggtcagta atatccacct
     2341 caacaatatc aggtaaatac gacggaagtg ctttaatatg catcagtctt tgattctttt
     2401 taagctttcc accagctttc acaccaatgc tttggccatt gataatcaag ggaacatgaa
     2461 ttgcaaccgg tttatcatcg ctcaactcca aaaaatcgat gtgatttact ttacttgaaa
     2521 tgggatcgta ttgaatgtct ttcataatag cctgatattt ctttttccca attttaatat
     2581 cgacaatgta aacattgtga ctataaatca gattcctcaa ttcggctctg gtagcagaga
     2641 aatttacatt ttcgccacaa ccgtacagga cacagggaac actatccagt tttcttaatt
     2701 tacgtgaagc atttttccca agatctgtgc gtttttctcc tttaatttcg aaatgttgca
     2761 taaaattatt ttttactgtg ttactaaagg taatggcaaa tttacgattt tcgtttctgc
     2821 acaatacctc ccctcacacg ttcgacaatc aggcggcgaa aataatgaat tattttgaat
     2881 tttaaaaact cattttttaa taggataatc cgttttccgc ctaaactata aaagtagtgc
     2941 tgatagattt atgtgtataa actttacgaa ttacatccgc aaaaaggctg gcaaccgata
     3001 gtacagtaat tttatcggaa agtggtttaa ctgggataga attggtaact accacttcgg
     3061 aaattgacga tttttctatt ctttcgtagg caggtccgga aagaacagga tgagtagcca
     3121 caactctgac acttttagca cctttttcca acataagatc cgaagccata cacactgttc
     3181 cggctgtatc aatcatatcg tcgacaatga ttacattttt gccttcaacc tcacctatta
     3241 ctttcatttc ggcaaccaca ttctcttcct ctcttgattt atgagcaatt gccattccgc
     3301 aattcagaaa gtgggaataa gctttagctc ttttgctgcc acccatgtcg ggtgaagcaa
     3361 taaccaaatc actcagatcg agacttttaa tataaggtac aaacaatgca gaggcaaaaa
     3421 tatggtcaac aggtacgtta aaaaatccct gaatctgatc cgcatgcaaa tccatagtca
     3481 taactcgatc gactccggag gccgagagta catcagcgat aacttttgca ccgatcgaaa
     3541 ctcgcggctt atctttgcga tcctgccttg caaagccgaa atacggtatc acagctacga
     3601 ccttataggc agacgctctc tttgcagcgt caaccataag catcagttca ataaggtttt
     3661 cagcaggtgg acaggttgac tggatgataa aaacggtgca acctctaact gtctcgttca
     3721 aactgggttg aaattctcca tcgctgaaac ggatcagagt acttttaccc aattccattt
     3781 ccgggtcata gaatgaaact atttcctctg acaggtatcg gctggcctgt cctgtaaaaa
     3841 attttatcgg tgattccatc aggtgaagtg ttgtttttgt ttcgacaaat gtaatgaaat
     3901 agccaaattg ggatagaaaa aatagaagaa acctttgtta aattatcaac agttatttca
     3961 ctattgttga gttctatata actatttgat tcacaataac caaagcatca aattaaaagt
     4021 ttaaaatatg atgtggatat cactgatttt acccggggtt cttaaaatcg ggcgatgccc
     4081 aaagggctgc catcaggtgc gtgatatatt attagtgtgc cccaaattat tcataatcac
     4141 aaagtgtgtc gattttttgc attaattccg cattttcttt ttctgttaaa gctgaatact
     4201 caacatagct caaaaacaat tcaatttttt cgacaacaca atagcaataa tcatctgctc
     4261 tgtttttatt ttcttttaaa ttatcattta aacatttact tgtaagggtt tgcttctgtt
     4321 cctctgtcca aggaacaaca tttctattgt tttttgcaaa atcagtcaaa tttgaattaa
     4381 tctgtccgtt ttttaaataa aatgtattct gggtaattac attgctatta taggttccac
     4441 tttccattag ttctcctgtt gcataaaaca tcttgtaatc gccatggcgc aatccgtttt
     4501 tataattccc ttcacttagt ttttgtccgt ttaaatagta gtacacccaa attccctcct
     4561 cttgatcatt tagtatttta ccggttttcc aaagcattcc attaggatgg taataaattg
     4621 tttctccttt tccgttttca aaaacgccta ttgatttgag agctccgttt tcgtaatagt
     4681 catgtaaggt atctgtcaat tgcccgttct tgtacatagc ctgttgccac aattgcccac
     4741 tgggatacca aaagatgctt tccccataga gctggttatt tttatagttg acccggtatt
     4801 tcatatgccc attatcgtaa taatataccg atacaccatt tctctgattg tgaaatattg
     4861 aaaaaacaaa attagtatca cctgtattat aaaaaccaat cagcttaata tccgaagtgt
     4921 cattggggtt aaggtattca attttagtct ttgcattacc atcagggtag ctatcaagaa
     4981 cctttgtgtt acaattctgg caaaccgatg ggccaattat cattaaaaaa cccagcgata
     5041 ataagaaaga ttctttcttc atgttagatt gatccctatt aatttagtat tgaaaagctt
     5101 cacaaaacac aataatttgt ttgctctcta aaagtttagt cgatagaagg cattatttca
     5161 ccatcatact cattcggatt tccttcaata attacgtttt ttgagcttgg gcgcctgtcg
     5221 atccaaagta tggcatcgag gtgatcgatt tcatgttgga aaatatgtgc tgtatattca
     5281 tgcgatattc tttcggtgca atgcgttcca tccggcaaat cgtattcaac atcgcaccat
     5341 attgcccgcc atgattgccc gctaactccg ggtatcgaca aacaaccgtc gttacgaagt
     5401 ttaagggtat cggaaagtgc tgttatcctg atatttataa aaacttccca gggtttcccg
     5461 accttatcat aacgttttac ccatacaact ctcctgttta ttcccacttg tggcgcagca
     5521 ataccaacgc cggggttctg ggggttcctt acagtagcgt acattctgtc ggtaagcaaa
     5581 gcaagttcct gttcggtaag ccggatatct gtgctgatgg ttctcaaaat aatcgaatcg
     5641 ggttgttgat tataatacat tatttccatt ctatcggcag aatcaccggc tgtgatcaat
     5701 gctttttcat ccgtagtaaa cttcagcgta tcgacagggg gcaaacgcaa ctgttcataa
     5761 ttatgtctgt cgttaggttc aaaagtttca catgctgtca gtgcaagaaa acacaaatta
     5821 ataacagtaa tcagtatggg gcaacttgct ttcatagtgc agattttaca ttacaaagat
     5881 tcaaatatac aaataccatt taataatgtg caatcgtttt catcggataa ttgttacagg
     5941 tcctttaaac ggagcacttt cacccatatc ggaatttagc attataatat accaatatgt
     6001 atcagctgca acaggttgtc gagcatagat tccgttccat ccctgctgat ttcccttata
     6061 ttttgcaact tgtttcccaa acctgtcgaa tattgtgata tctgcgttcg gatatctgaa
     6121 tattccaata atttcccagg tatcgttata gccatcacca ttaggcgtga aaaactcagg
     6181 aataattata ttattatcga cataaaccct gattgtatct ttgcaggaat tgctatcgga
     6241 aacatataca ttataccagt cttcgagcag ttgattaaaa gaattcactg gcgaccagta
     6301 taagccatcg acagaatagc tgtaaggggg cgttccgccg gccgcattca ctacaattct
     6361 gcccgaagca ctgaattctt ctgtaagcac agggctatcg gcatgactaa ttgtaatatt
     6421 gtcggaactg gtgcagccat taaagtcagt cacagtaaga gaataaacac ccggatatac
     6481 agcaagctgt acggtatcgt ctgatccatt ttgccatagg taacttttaa aatatccatc
     6541 agggtctaac aacaaagggg cactatcgca tgaaacaaaa tcaaaaccga ggaacggggt
     6601 ttcaacctca aagaagttca cttctacagt atcgacagca gtgcaaccat attcatcatg
     6661 cactgtaagt gagtattcgc ctgactcggt aacattaaga atgggcatag aggctcctgt
     6721 actccactca tatattgtaa atccggatgg aggacttagc gaaacttcat tatttgcgca
     6781 agtctgcaca tctgctccga ggctcacttc aggagccggt cttaccgtca ctgaaatagt
     6841 atcggctgca gtacaacccg tttgatttgt tacagttact gaatacgtcc ccgaagtctc
     6901 aggactaatt gttgccccgg agcttgcatc ggaccacaaa tagctaacaa agccacttcc
     6961 ggcatcaatt acaggtatat cattatcgca gtactcctgg tctggtccca gattaacttc
     7021 ggggtactct cccagtgata cgtatatact atcgtatgcg ctacaaccat tggcatcggt
     7081 tacggtataa gt
//
LOCUS       DAOOQP010000139         7046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_13121_length_7046_cov_4.332284, whole genome shotgun sequence.
ACCESSION   DAOOQP010000139 DAOOQP010000000
VERSION     DAOOQP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13121_length_7046_cov_4.332284"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PLK75_11515"
     CDS             complement(<1..1807)
                     /locus_tag="PLK75_11515"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39914.1"
                     /translation="MKNTFRNRMRYAFDNFMSKGTIALILGLSVVTIIIILLAAFFVH
                     VCAIAQPGTGEMGFFEAFWQSLMRTLDAGNLAGDEGWDWRLVMFIVTLGGIFILSALI
                     GVLNTGLNTKLHELRKGRSLVIEKNHTVILGWSAKIFTILSELIEANSNHKNCRIVIL
                     GDKEKVEMEEAIHDKIKNFGTTKIICRSGRPIIMNDLKMVNLPDARSIIVLAPEKKDP
                     DSEVIKTVLAITNDPERATKKYHIVAEINDFKNADVCRMVGKDEIEIVLSTEIISKIT
                     AQTCRQSGLSAVYMELLVYEGDELYFQKQPEFIGKTYGEALFAYDDSSLFGLYTASGE
                     TLINPLMNTVIGPDDEVICISQDDDTVIFNGRPFEIQEKYIQKPSSHNLKKENTLILG
                     WNSRGSLIIKELDAYVEKGSQITIAANVKKLDQQAQTEIESLCNCSLNIIICDTTNRK
                     AIETLNLTLFDHIILLSYAGLMSPEESDSATLITLLHLRDIAEKTGLEMSIVSEMLID
                     DNRILAEVTNADDFIVSEKVISLLLTQISEKKELAKVFNNMFSPEGSEIYLKPVTNYI
                     TAGIETDFYTLLEAARRQNETAIGYRLIRYKHDSAR"
     gene            complement(1938..3128)
                     /gene="kbl"
                     /locus_tag="PLK75_11520"
     CDS             complement(1938..3128)
                     /gene="kbl"
                     /locus_tag="PLK75_11520"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOY39915.1"
                     /translation="MYGKMQEYLQTELKNIREAGLYKNERIIISPQKAEIKVNSGQEV
                     LNFCANNYLGLSNNPKLIEAAKKALDTHGYGMSSVRFICGTQDLHKTLEAKIAEFFGT
                     EDTILYAACFDANGGVFEPLFNEQDAIISDSLNHASIIDGVRLCKAERYRYNNADMAD
                     LEEKLKEAQKHRFRIVVTDGVFSMDGNVAPMDKIVALAEKYDAMVMVDECHSAGVVGK
                     TGRGVTEEFNVRGKVDIITGTLGKAFGGAIGGFTTGRKEVIELLRQRSRPYLFSNSIP
                     PSVAAAGIKMFDMMSETHELQDKLHRNTEYFSAKMQAAGFDCKPTKSAIIAVMLYDAK
                     LSQDFAAKLLEEGIYVIGFYYPVVPKGLARIRVQVSAGHEIEHLDKCIAAFTKVGKEL
                     GVIK"
     gene            3412..4374
                     /locus_tag="PLK75_11525"
     CDS             3412..4374
                     /locus_tag="PLK75_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY39916.1"
                     /translation="MKNILVVGSAGQIGSELVLALRNIYGGNHVIAGIRKTKPSEKLT
                     STGPCEVVDANDVNNLVSVVKKYDVDVIINMAAILSATGEQNPMMAWNVNMNGLINIL
                     EVAREFKMEQVLVPSSIAVFGPTTPVDNTPQETILKPTTMYGVTKVAGELLGDYYVRR
                     FGLDVRGLRYPGIISNETLPGGGTTDYAVAIYYEAIKNKKYTCFVNAETRLPMMYMPD
                     CLKATIDLMQADFNKLKHHCDFNVGAMSFSTAELAASIKKYIPEFEISYEPDFRQKIA
                     DSWPNSVDDTAARQEWGWKPSFDLDAMTQDMLKVIGEKHSKGLI"
     gene            complement(4692..>7046)
                     /locus_tag="PLK75_11530"
     CDS             complement(4692..>7046)
                     /locus_tag="PLK75_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY39917.1"
                     /translation="YFTSMRKGGFGGMDIYYSELDKHGKWGPAVNLGPTINTVGYEEM
                     PYISRDGTELYFSSEGHTTKGGFDVFYSKLDTASGKWSEPENVGYPFNTDGNDMGYIV
                     EFNGDVSFCPVNSSKRRGGYEECDCIRIKLGQGAPLITCKVVNDNTIPEFPDDVMLFV
                     INDATKDTVGTYNISESKDHTALFILPQGKYNLYTQSDEALPTLTVLEIPERTGPIDT
                     TIVISSPQAPQAPVVCVVKHDNTLPKLPNDVMLYVINDNTKETIGTYNVTDRKNSVTL
                     PLPPGDYTFRAESDSAYPASKSLHVPETHDTLTVVVTISAPSDKYLQITWVQFDFDKS
                     DIKPEYYANLDSLADYMKRYPNAVIYVNGHCDPFGTNEYNIALGDRRANSVKTYLVSK
                     GVNPDNLITKSFGEEELIARGESAEARVYDRRVEFIVKQQGDVELRVVPKNPPAKYAL
                     DGKGEEPQNIQEPQQGTVKEPIVADPPKGTEQKVVVTWVQFDFDKSDIKPESYENLDS
                     LADYLVTYPEAKVYVHGHCDPFGTNAYNIALGDRRANSVKNYLTSKGVNSNQLLTDTY
                     GEERLIAKGESAEARVYDRRVEFIVIKQGGIDLIVVPKLPPAKYALDGQSYSQDNNTV
                     PPASNQPVNTNTTETAPADSEFGTVNPDIILEITWVLFDFDKSNIKPEFYANLDSLAD
                     YLKKYPEARIYVHGHCDPFGSNAYNIALGQRRANSVSAYLINKGVKSEQLVPETFGEE
                     RLNAKGESAEARIYDRRVEFIVIKQGGITLKVIPRKPPAKYAID"
BASE COUNT         2006 a         1451 c         1426 g         2163 t
ORIGIN      
        1 ttcttgctga atcgtgtttg taccgtatca ggcggtatcc gattgccgtt tcgttctgac
       61 gtcgggctgc ttctaacaaa gtataaaagt ctgtttcaat tcctgcagta atgtagtttg
      121 taacaggttt taaatatatc tccgagcctt cggggctaaa catattattg aataccttgg
      181 ccaattcttt cttctcgcta atttgtgtta gcagcaaact aattactttt tcgcttacaa
      241 taaagtcatc ggcattggta acttctgcca gaatacggtt gtcgtcgata agcatttcgc
      301 tgacaatgga catttccagc cccgttttct ccgcaatatc tcgtaaatga agaagggtta
      361 ttaaggttgc tgaatctgac tcttcaggac tcatcaatcc tgcatagctt aaaagaataa
      421 tatggtcaaa caatgtcaga ttcaatgttt ctattgcttt tcggttggtc gtgtcgcaaa
      481 taatgatatt taggctgcaa ttgcatagac tttcgatttc ggtttgagct tgctgatcga
      541 gttttttcac attagcagca atagttattt gcgatccttt ttctacatat gcgtccagct
      601 ctttaataat cagagagcct cgcgaattcc acccaagaat taacgtattt tcttttttca
      661 ggttatgcga ggagggtttt tgaatatatt tttcttgaat ttcgaaaggt cttccattaa
      721 aaataaccgt atcgtcgtct tgagaaatgc aaattacttc gtcatccggt ccaataactg
      781 tattcatgag aggattgatc agtgtttccc ctgaagcagt atataaaccg aaaagactag
      841 aatcgtcgta tgcaaaaaga gcttctccat aggttttgcc gataaactca ggttgtttct
      901 ggaaatatag ttcatctccc tcatacacca aaagctccat ataaactgcc gaaagccccg
      961 attgacgaca ggtctgtgct gtaatctttg atatgatctc cgtagaaagg acaatttcaa
     1021 tttcgtcttt tccaaccata cgacagacat cagcattttt gaaatcgttg atttcggcaa
     1081 cgatatgata tttttttgta gctctttcgg ggtcattggt aatggcaagt accgttttaa
     1141 ttacttcaga atcaggatct ttcttctccg gagccagcac aattatagaa cgggcatcag
     1201 gcaggtttac catttttaaa tcgttcatga taatggggcg tccactacgg caaataatct
     1261 ttgtagtgcc gaagttcttt atcttatcat gtatggcttc ttccatttcc actttttctt
     1321 tatcccccag aataacaatg cggcagtttt tatgatttga attggcttcg ataagttcac
     1381 ttaaaattgt aaatattttg gcagaccatc caagtatcac ggtatggttt ttttcgataa
     1441 caagcgatct gcctttgcgg agttcgtgaa gttttgtatt taaccccgta ttcaacacac
     1501 caataagagc gcttaggatg aaaataccac ccagcgttac aataaacatt acgagccgcc
     1561 aatcccagcc ttcgtcacct gccaaattcc cggcatccaa cgtccgcatc aggctttgcc
     1621 agaaagcttc aaaaaacccc atctcaccgg tgcctggctg agcaatggca caaacatgca
     1681 caaaaaaagc cgccagtaga attataatta ttgtaaccac cgacagcccc agtattagag
     1741 caatggtgcc tttcgacata aagttgtcga aagcatagcg catacgatta cgaaatgtgt
     1801 ttttcatcca aagcgttttg tttaacaaat gtataaaatg tttaacaaat ttaacctgcc
     1861 actcatatta tttgtattta cataaaaaag gagggcagta aaactgccct ccttgaaaat
     1921 atttgattat tggtagttta tttaattaca ccaagctctt ttccaacttt ggtaaaagca
     1981 gcgatacatt tatcgaggtg ttcaatttcg tgtccggccg atacctgaac gcggatacgt
     2041 gccaaaccct taggaactac cgggtagtag aagccaatta cataaattcc ttcttcaaga
     2101 agtttagctg caaaatcctg tgataatttg gcatcgtaaa gcattactgc aataatagcc
     2161 gatttggttg gtttgcaatc aaacccggca gcctgcattt ttgcggagaa atattcggtg
     2221 ttgcgatgaa gcttatcctg taattcgtgg gtttccgaca tcatatcgaa cattttgatt
     2281 ccggcagcag ctactgaagg gggaatagag tttgaaaaaa gatagggacg cgagcgctga
     2341 cggagtaatt cgataacttc tttacgacct gtggtaaatc cgccgatagc accaccaaat
     2401 gctttaccca atgtaccggt aataatgtca accttcccgc gaacattgaa ttcttcggta
     2461 acaccacgac cagttttacc aacaacaccg gcagaatggc attcgtcaac cattaccata
     2521 gcgtcgtatt tttcggccag ggctacaatt ttatccatag gagccacatt gccatccatt
     2581 gagaaaacac catcggtaac tacaatgcgg aaacgatgtt tctgtgcttc cttgagtttc
     2641 tcttccaaat cagccatatc ggcattgttg tagcgatatc tttcggcttt gcagaggcgc
     2701 acaccatcta ttatcgaagc gtggttcaat gaatcggaaa taatagcatc ctgttcgttg
     2761 aataatggtt caaaaacccc accgtttgca tcgaagcagg cagcatacag aatagtgtct
     2821 tccgttccaa aaaactcggc tatttttgct tccagcgttt tgtgaaggtc ctgggttccg
     2881 caaataaagc gaaccgacga cattccgtaa ccatgagtat cgagagcctt tttcgctgct
     2941 tcgataagct ttggattatt cgacaagcct agataattgt tagcgcagaa attcagaact
     3001 tcctggcctg aatttacttt gatttcagct ttttggggag aaataattat ccgctcgttt
     3061 ttgtacaaac ctgcttcgcg aatgtttttt aattctgttt gcaggtactc ttgcattttt
     3121 ccgtacatag taatttgatt tttagatgtt attgaatgtt gaatacagtt taaatttcag
     3181 tgctcaaata tagttttttt ggtaatcgct gatacttcaa aaaagcaaaa ttttgacagg
     3241 gaaatgaata ttatcggcga catcggaata taatttttca aagcataaaa atatggtgtt
     3301 tataaattga taattaggat ttttatcata ttttcggaac cggatttgaa ttagttttga
     3361 cgcgcaaaat gttaggtata ttgtatatta ataattaata aacacaacga aatgaaaaac
     3421 atcttagtag taggatctgc aggtcagatc ggttcagaac tcgtgctggc actgcgaaat
     3481 atttacggtg gcaatcacgt gattgccgga atcaggaaaa ccaaaccttc cgaaaaactc
     3541 acatcaacag gtccctgcga agttgtcgat gccaacgatg tgaataacct cgtgtcggtt
     3601 gtaaaaaaat atgatgtgga cgttatcatc aatatggcag ccattctttc tgctacggga
     3661 gaacaaaacc caatgatggc atggaatgtt aacatgaatg gcctgatcaa tattcttgaa
     3721 gttgcccgtg aatttaaaat ggaacaggtg cttgttccca gcagtattgc tgtttttggt
     3781 ccaaccacac cggtcgataa cactcctcag gaaacaattc tgaagcccac taccatgtat
     3841 ggcgttacta aagttgcagg cgaattatta ggcgattatt atgttcgtcg tttcgggctc
     3901 gatgtacgtg gtttacgtta tcctggtatc atcagtaacg aaacccttcc cggtggcgga
     3961 actaccgatt acgcagtagc tatttattac gaagcaatca aaaacaaaaa atatacttgt
     4021 tttgtaaatg ccgaaactcg ccttccgatg atgtatatgc ccgactgcct gaaagctacc
     4081 atcgacctta tgcaggctga ttttaacaaa ctgaagcacc actgcgattt caacgtggga
     4141 gcgatgagct tcagtaccgc tgaactggct gcttcaataa agaaatatat tcccgaattc
     4201 gaaatttcgt atgaacccga tttccgtcag aaaatagctg actcctggcc aaattcagtt
     4261 gacgatacag ctgcacgaca ggagtgggga tggaaaccaa gtttcgatct tgatgcaatg
     4321 actcaggata tgctgaaagt gatcggcgaa aagcattcta aaggtttgat ttaagcaaaa
     4381 attacacaat tgaaaaggag aggagctgca acattgttgt ggctcttttt gtttcataac
     4441 cataaaatac aatcgaaaac cctcagtaag tttgagagcc cgtcttagaa tacgaatgtt
     4501 agatacgaac atgaagcaat ctgtatttct acgggcgaat ttaaaaagtt cgttaatacg
     4561 gtgatttaaa ttcgtttatt ggtggattac aacgacgatt tgagaaaagg aataacgaaa
     4621 ttgaataaat gactaaaaaa agagctgtca taaatagttg acagcccttt ttaaaaaaaa
     4681 tctaaaacgg gttaatcaat tgcatacttg gcaggaggtt ttctgggaat cactttcagg
     4741 gtgataccgc cttgtttgat aacaataaac tcaaccctgc ggtcgtaaat acgtgcttcg
     4801 gcgctttcgc ctttggcatt taatctttct tctccaaatg tttcgggaac cagttgctcc
     4861 gatttaactc ctttattgat taaataagct gaaacagaat ttgctctgcg ttggcccaac
     4921 gcaatgttat atgcatttga accaaatgga tcacaatgtc cgtgaacata aattcttgct
     4981 tccggatact ttttcaggta atcggcaagt gaatcgagat tcgcatagaa ttcgggttta
     5041 atgttgctct tgtcgaagtc gaatagaacc caagtaatct caaggattat atcagggtta
     5101 acggtaccaa attctgaatc cgccggtgca gtttctgtag tatttgtatt tacaggctgg
     5161 tttgaagccg ggggaaccgt attattatcc tgagaataac tttgtccgtc aagtgcgtat
     5221 tttgcaggag gtaatttggg aacaacaatc aaatcgatac cgccttgctt gattacaatg
     5281 aattcaaccc tgcggtcgta aacacgtgcc tctgcacttt cgcccttggc aatcagtcgt
     5341 tcttcaccgt aagtgtcggt aagcaattga tttgagttaa cacctttact tgtgagataa
     5401 ttcttcactg agtttgcgcg acggtcgcca agtgcaatgt tgtatgcatt ggtgccaaac
     5461 gggtcgcaat gaccatgaac ataaactttt gcttcgggat atgtaaccag atagtcagct
     5521 aaagaatcca gattttcgta ggactcaggt ttaatgtcgc tcttatcgaa atcgaattgc
     5581 acccaggtaa ctaccacttt ttgttcagta ccctttggtg gatctgctac aataggttct
     5641 ttaacagttc cttgttgtgg ttcctgaatg ttctgaggtt cttctccctt gccatcaaga
     5701 gcatacttgg caggaggatt ctttggcact acccgaagtt caacatcgcc ttgctgttta
     5761 acaataaact ctactctgcg gtcataaact cgggcttctg cactttcgcc acgggcaatc
     5821 aattcttctt caccaaacga tttggttatc agattgtcag gattaactcc tttacttact
     5881 aaataggtct ttaccgaatt tgcacgacgg tcgccaagtg caatgttata ttcgttagtt
     5941 ccaaatgggt cgcagtgacc gtttacatat ataactgcat ttgggtaacg cttcatgtaa
     6001 tcggcaagag aatcgaggtt tgcataatat tcgggtttaa tgtcgctctt atcaaagtcg
     6061 aactgtaccc aggtaatctg gagatacttg tcgctgggag ccgagatggt aacaactact
     6121 gttaatgtgt cgtgggtttc aggtacatgc aacgattttg aggcagggta agcagaatcg
     6181 ctttcagccc tgaaagtgta atcacccgga ggcaaaggaa gcgttacact gttttttcta
     6241 tcggttacat tataagtgcc gatggtttct tttgtattat cgttaatcac ataaagcata
     6301 acatcgttcg gaagtttagg cagtgtatta tcgtgtttaa cgacacaaac aaccggagcc
     6361 tgaggagcct gtggtgatga aataacaatg gtagtatcta taggtccggt gcgttcaggt
     6421 atttccagga ctgttagagt aggtaatgct tcatcgcttt gggtgtacag attatatttt
     6481 ccctgtggca gtataaataa agctgtgtgg tcttttgatt cactgatatt gtaagttccc
     6541 acagtgtctt ttgtggcatc attaattaca aatagcataa catcatccgg aaattcagga
     6601 atagtgttgt cgttaaccac cttacaggta atgagtggag caccctgacc gagtttaata
     6661 cggatgcaat cgcattcttc gtaaccaccc ctgcgtttgc tactatttac agggcagaac
     6721 gaaacgtcac cgttgaattc aacaatatat cccatatcgt taccatcggt gttgaaagga
     6781 tagcctacat tttcaggttc cgaccatttg ccgctggcag tatccaattt cgaataaaaa
     6841 acatcaaaac cacctttagt ggtatggcct tcagaactaa agtacaattc agtcccatct
     6901 ctcgaaatgt acggcatttc ttcgtacccc acagtgttga tggttggacc aaggtttaca
     6961 gctggtcccc atttaccgtg tttatcaagc tctgaatagt aaatgtccat gcctccaaat
     7021 ccgccttttc gcatacttgt aaagta
//
LOCUS       DAOOQP010000140         7025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_13172_length_7025_cov_6.004304, whole genome shotgun sequence.
ACCESSION   DAOOQP010000140 DAOOQP010000000
VERSION     DAOOQP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13172_length_7025_cov_6.004304"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PLK75_11535"
     CDS             complement(<1..1276)
                     /locus_tag="PLK75_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39918.1"
                     /translation="MKNLFTSGKAIVGILMLIALLSGSLNGYSQCTNCGTNYPSGTTT
                     GAPIAWTTRAGCNYSGEYAYYTLTTGYIYQWGSQGTTYDTQLTLYPSGTCTSGYQLAY
                     NDDFNGNTSLISYEPGTTSVRVLFSKYSCVAYGSGNTCGYQLYRAIPKTPTITNSNTS
                     ICTGGSSTLSANSGLVDDVYMDCYWGTSAGGNNVGSSVASVLVSPTTTTTYYLRYLVY
                     GGSAGTRYSSDASTTVTVVADPSISISANYTNICTGGSVTFTQSVSGGTGTITNQWQY
                     STDNVNWTNWTTTTNPTYSGLTQTMYFRCIRSATASGCDTYTSNTVTVTVNADPYITA
                     HPTGATICTGNSHSMSVTAAGGSSAGLTYQWQSSVIGAGSWTNASTGSSLVATPPYPG
                     RDYRCLVSGTAAHGCSVNVTSNTATVYVDAATV"
     gene            complement(1296..1667)
                     /locus_tag="PLK75_11540"
     CDS             complement(1296..1667)
                     /locus_tag="PLK75_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39919.1"
                     /translation="MKNCLMTLVLLVIAAISFGQNVDKLYTDDMSYDEKIAAGKAYID
                     ETFPDCDFDYFAPNCIYNKKFSSEDERQTMKKDWFEKFRYEYELCEYYNSKKANSQMQ
                     ENDMQLREKEQIEKEKQHRNK"
     gene            1904..4264
                     /locus_tag="PLK75_11545"
     CDS             1904..4264
                     /locus_tag="PLK75_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39920.1"
                     /translation="MKLSSGHTKIVLLFALLIITVFVYFKVTGYGYVNWDDDENIRAN
                     PRFTEFSSENLVYHFEHSRFKTIAIWSYMAQYQLFGPDAGSFHTVNLILHLCNIVLVF
                     FFVKRILPAKKFTALIVAALFALHPAFVEPVVWVTGRKDLLFVMFSLLSFLAYVRFVN
                     TTNGIKKYLFFAFTMLCVYLASMAKIQAVALPVVFIAIDFLYQRKTRVFNLAEKLILL
                     LICFDYFLPVIALLLLGLMLKFYKHLIGIITKSKSSKIISIVLLLLVVSALQMPFKYF
                     LKEFPIFAIEGFNNNLILWIIYLIFISYVIFNKFFDRIANRLKENGVNKHRKWIAPVF
                     FISIIFVVVIKFDSILSQFTGLWNYNSGSEHFYSIWERILFLSGSMVYYLKRFFLLQA
                     QDPMIPYPERGINGAMPDYLFTDFIAVLIIVALSAFVLFRYFRKNRVVLFGVAFFLIN
                     IGLVLHIIPIEGRVLVADRYTYLAYIGLFLLIGLLADRLAERQKRPAIIAGAGGLILL
                     FVGFTTYRDKAAWENSFTLWQKALVVNPENHYAMYSLALAYFSEGQNASTAENYLNEA
                     ILMKQDFMYYNNRGRVRYSLGNLRGALSDFDKAIAMDSTNFASYNNRGAVRQQFCDYR
                     GALIDYAEAIRIFPDYQEAKNNKRKVEELILLDSLLFAWNDTLSVPRERLTDFVLMAT
                     EKLISSEQTEKALLYLDAGMKIYPAEARFYEKTAVIFHLKNQYAEAKKYYDRGLQEVP
                     ENTSLLLGRGLLFIQTGDTAKACIDLQIAAEKGDADAKNLLQQFCK"
     gene            complement(4302..6848)
                     /locus_tag="PLK75_11550"
     CDS             complement(4302..6848)
                     /locus_tag="PLK75_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39921.1"
                     /translation="MGKKAEKAKFFLKRKWVFFFLSVLILIVYAKSFQNGYNIDDNYV
                     IENHQLAQQGIKAIPEIFTSRYHTQDNLYFGYRPLTVAIYAIEWDFFVNFVGVGADNF
                     PHVGHIINVIYYIITCCLLYYTIALLFNKTPYGNLLALISAIIFVVHPVHTEVVLSLK
                     NREELICTILSLIATIQAIRYYDSKKIWRALLTILCLTFAFLAKESAVIFLLIIPITV
                     IFFRSELHLNYKFDWKQKETYYNAGFWTLALLYIFSLKWFSLGGFKADNILFILPEKL
                     NKICLFGFILSYLIFNIIASIRSKFSAIYKPDLNSAYFYFSTACIILAISISSSILAA
                     LFLFISCIFITRKTIIFQTRPDEAGIDKTSNIKSLFKNKLVVASLIVILVSGAAILSI
                     YYAQNVSLPEKNAPVFKWQNPVFDFTKSSSEKLSIALYSFGYYSKLLLIPFPLRFYYG
                     YRMIPDSNLSHPVVILSVIMLLALLAVALITFNKRSPLSYGILFFLITIIPFSNFVFP
                     LTGIVAERLLFIPSIGFCVIVSYLIILLLKSHKTVPDTKKQFTRPLLLSLVVVIPFSV
                     VSVNRNDDWKDRPTLYAHDIIYLSNSAKANNLYANYLIAEVYARIQTNTDISPVKENI
                     NLAINYYKRAVEIDSTYANPLHNLGYIYLILKEDFPLAKTYFNKCIALDTTIDEAILN
                     RGIANYHLHLTDSCITDLDHYMKISGKMPDKAYYYLAKAYIDKNNHPIAADYFIKLLE
                     TNKTTRKINDEIKTYFFERKDYTHAILAAQNQIRFDPNSDLPYVDIGNYYLLSGDTVQ
                     AIVNWETAFENFNGNYNIGMTLSGYFAGIGNTAKAQYYYQKAIAFRQKHK"
BASE COUNT         2084 a         1255 c         1471 g         2215 t
ORIGIN      
        1 ctacggtagc tgcatcaaca taaactgttg cggtatttga ggtaacattt acactacacc
       61 catgagcagc ggttcccgaa acaaggcatc ggtaatcgcg ccccggataa ggtggggttg
      121 caacgagact gctccctgtg gaagcatttg tccagctacc tgccccaata acgcttgatt
      181 gccattggta cgttaggccg gctgaagatc ctcctgctgc agtaaccgac attgagtgtg
      241 agtttccggt acaaattgtt gctccggttg ggtgagcggt gatgtatgga tcagcattaa
      301 cagttactgt aactgtgtta gaagtgtagg tatcacaacc acttgctgtt gcagagcgaa
      361 tacagcggaa gtacatggtt tgggtgagac cggaataggt tgggttggtt gtggttgtcc
      421 agtttgtcca gttcacatta tcggtagaat actgccattg gttggtaatg gttccggtgc
      481 cacctgaaac agattgtgta aaggttacgc ttcctccggt acaaatattt gtgtagttgg
      541 ccgatatgga gattgacggg tcggcaacta ccgttactgt ggttgatgcg tcactagaat
      601 atcgggtacc ggcactacca ccatatacga gatatcgtaa atagtaggtt gtggtagttg
      661 taggggaaac cagtacagaa gctacactag aaccgacatt gttaccgcct gccgatgtac
      721 cccaataaca atccatataa acatcatcaa ccaagcctga attggccgac aaagttgatg
      781 aacctcccgt gcaaattgat gtattcgagt tggtgattgt aggtgttttg ggaatagccc
      841 ggtacagttg gtacccgcat gtatttccac tcccataggc aacgcaacta tatttcgaga
      901 agagaactcg caccgaagtt gttccgggtt catacgaaat taaagatgtg ttcccattaa
      961 aatcatcgtt atatgccaat tgatagccac tagtacatgt gccgctgggg tacagggtta
     1021 actgtgtgtc gtaggtggtc ccctggcttc cccattggta gatataacct gttgtcagtg
     1081 tgtaataggc atactctccg gaataattac aacccgcacg cgttgtccaa gcaattggag
     1141 cacccgttgt tgttccggat gggtaattag tgccgcaatt cgtacattgc gagtacccgt
     1201 tcagtgagcc tgataatagg gcaatcagca tcaaaatccc aactattgct ttacctgaag
     1261 taaataaatt cttcatattt attattttaa ttcaattatt tattacgatg ttgtttttct
     1321 ttttcaatct gttctttttc ccgcaactgc atatcgtttt cctgcatttg tgagtttgct
     1381 tttttgctgt tatagtattc acacagttca tattcgtagc ggaatttttc gaaccagtct
     1441 tttttcatgg tttgcctttc atcttcgctg gaaaattttt tattgtaaat gcagtttggt
     1501 gcaaaataat cgaagtcgca atcggggaaa gtttcatcga tataagcttt gccggcggca
     1561 attttttcgt cgtacgacat atcgtcggta tagagtttat caacattctg cccgaaagaa
     1621 atagcagcaa taaccaataa aacaagcgtc attaaacagt ttttcataat gcaaaacata
     1681 attaaacaat aattctaaat tacggcaaat ttacgaaatt gcatgagtgg cacgtaaaaa
     1741 cattcgtttt ttttttaata attatactat taaagatgcc caccaacaag atgatgaaat
     1801 atttatataa acttatgatt atgaaaatgt tgggcaaagg tatgcaaact cgtttttttg
     1861 taatagtttt gcatgaattg tttttcatta aaaacacagc aagttgaaac ttagctccgg
     1921 ccatacgaaa attgtgcttc tctttgcgct gctgattatt acagtatttg tttacttcaa
     1981 agtcacaggt tatggttacg tgaactggga tgatgacgaa aacattcgtg caaacccccg
     2041 ttttacggag ttcagctccg agaacttagt gtatcatttt gagcattctc gatttaaaac
     2101 cattgcaata tggtcgtata tggcgcagta tcagttgttt ggacccgatg ccggtagctt
     2161 tcatacagta aatctgatat tgcatttgtg caacattgtg ctggttttct tttttgtgaa
     2221 gcgcatactg cctgctaaaa agtttacagc attgatcgtt gcagcgttat ttgctttaca
     2281 cccggcgttt gtagaacctg ttgtgtgggt taccggccgt aaagacctgt tgtttgtaat
     2341 gttcagtctg ctgtcgtttc tggcttatgt tcggtttgtt aacactacaa acggtatcaa
     2401 aaaatatttg tttttcgctt tcacaatgct atgtgtttat ttggcaagta tggcaaaaat
     2461 tcaggcagtg gcattgcccg tggtgttcat agccattgat tttctgtatc aacgtaaaac
     2521 cagagtattt aatttggctg agaaattaat cctattgctt atttgtttcg attatttttt
     2581 gccggtcatt gcgttgcttc tattaggctt gatgctgaaa ttttataaac accttatcgg
     2641 gataattacc aaatcaaaaa gttcaaaaat tatttcaatt gtgttattgc tactggttgt
     2701 ttctgctttg caaatgccat ttaaatattt tttaaaagag tttccaatat ttgctatcga
     2761 aggtttcaat aacaatttaa ttttatggat tatttatttg atttttatta gctacgtcat
     2821 ttttaataaa ttttttgaca gaattgcaaa tagattgaaa gagaatggtg taaataagca
     2881 caggaagtgg atagcacctg ttttttttat ttctataatc tttgttgttg ttattaaatt
     2941 tgattctatt ctatcacaat ttacaggatt gtggaactat aactccggaa gtgagcattt
     3001 ttatagcatt tgggaaagaa tcttattcct aagcggtagc atggtgtatt accttaagag
     3061 attttttctg cttcaggctc aggatcccat gattccatat cctgaaaggg ggattaacgg
     3121 ggcgatgccc gattacctat tcactgattt tattgcagta ctaataattg ttgcactaag
     3181 tgcatttgtt ctgttcagat actttcgcaa aaatagggta gtgctctttg gcgtagcgtt
     3241 tttcctgata aacattgggc tggtgctgca tattattccc atcgaaggac gtgtgctggt
     3301 ggcagaccgg tacacatatc tggcatatat cggacttttt cttttgatag gtttgcttgc
     3361 cgaccggctg gctgaaaggc aaaagagacc tgcaattatt gcgggcgctg gcggattaat
     3421 attgctgttt gttggattta caacataccg cgataaagct gcctgggaga attcttttac
     3481 cctttggcaa aaggcactgg tcgtgaatcc ggaaaaccat tatgccatgt atagccttgc
     3541 gcttgcatac ttcagcgagg ggcagaacgc gtctactgcc gaaaactatt tgaacgaagc
     3601 catcttgatg aagcaggatt ttatgtatta caacaaccga gggcgtgtgc gatattcgtt
     3661 aggcaatctc aggggagcct tgtccgattt cgataaagcc attgcgatgg attcgactaa
     3721 ctttgcatcg tataataaca ggggagccgt aaggcaacaa ttttgcgact acaggggggc
     3781 gttgatagat tatgcagaag ctatacgcat attccccgat tatcaggagg ctaagaacaa
     3841 caagcgcaag gttgaagaac tgatattgct tgacagcctg ctctttgcat ggaacgacac
     3901 cctctctgtt ccaagggaaa gacttacaga ttttgtgctg atggctacag aaaagctgat
     3961 tagttcagag cagactgaaa aagcccttct ttacctcgat gcaggaatga aaatataccc
     4021 cgcagaagcc cgcttttatg aaaaaactgc ggtaatattt catttgaaaa atcaatacgc
     4081 cgaagcaaaa aaatattatg atagaggtct acaggaagtt cccgaaaaca cctcgcttct
     4141 gctgggaagg ggactcttgt ttattcaaac aggagacaca gccaaagcat gcattgattt
     4201 gcagattgcg gccgaaaaag gtgatgctga cgcaaaaaac ctcttgcagc agttttgtaa
     4261 ataattccac gaattaattt caccgttttt tttggtaagg actacttgtg tttttgcctg
     4321 aaagcaatgg ccttctgata atagtattgt gcttttgcgg tgttgccgat acctgcaaaa
     4381 taccctgaca acgtcatacc aatgttataa ttgccattga aattttcaaa cgcagtttcc
     4441 cagtttacta tggcttgcac tgtatcgcct gaaagcaggt aataatttcc gatatcaaca
     4501 tacggcagat cgctgtttgg gtcaaagcga atttgattct gtgctgccaa aatggcatgt
     4561 gtatagtctt ttctttcgaa aaaataggtt tttatttcat cattaatttt gcgggtggtt
     4621 ttgttggttt cgagcagttt gataaaataa tccgcagcaa tagggtgatt atttttgtcg
     4681 atatacgctt ttgcaagata gtagtatgct ttgtctggca ttttacccga gattttcata
     4741 taatggtcaa ggtctgtaat acagctatcc gtaaggtgca ggtgatagtt tgcgattccg
     4801 cggttcagta tggcttcgtc aatagtggta tcaagtgcga tacatttgtt gaaatatgtt
     4861 tttgccaatg gaaaatcctc tttcagaatc agataaatgt atcctaaatt gtgcaatggg
     4921 ttggcatagg tagaatcaat ctcaaccgct cttttataat aattgatggc aagattaata
     4981 ttttctttaa ccggcgatat atcggtattc gtctgtattc gggcatacac ttccgcaatg
     5041 aggtaatttg catacagatt atttgcctta gccgagttgc tgagatatat tatatcgtga
     5101 gcatacaatg tgggacggtc tttccagtcg tcgttcctgt ttaccgaaac caccgaaaat
     5161 ggtatgacaa ctacaagcga aagaagaaga ggtcttgtaa attgtttttt tgtatcaggg
     5221 acagttttgt gcgattttaa aagtagaatt atcaggtacg aaacaatgac acagaaacca
     5281 attgatggta taaacagcag tcgctcagca acgatcccag tgagcggaaa aacgaagttc
     5341 gaaaagggaa taattgtaat taaaaaaaag agtattccat agcttaaagg ggagcgtttg
     5401 ttaaatgtaa ttaatgcaac ggcaagcaaa gcgagcagca taataacact caatattaca
     5461 accggatgcg ataaattgga atcaggtatc attctatatc cgtaataaaa acgaagaggg
     5521 aatggtatga gcagaagctt ggaataatac ccaaatgaat acaaggcaat cgatagtttt
     5581 tcggagctgg attttgtgaa atcgaacacc ggattctgcc atttaaaaac cggtgcattt
     5641 ttctcaggta acgaaacatt ctgtgcataa taaatggata gtatcgccgc tcccgatact
     5701 aaaatcacta ttagcgaagc gaccactagt ttgttcttga aaaggctttt aatattgctg
     5761 gttttatcta tgccggcttc atctgggcgc gtttgaaaaa tgatggtttt tctggtaata
     5821 aatatgcaac tgataaataa aaagagggct gcaagaatgc tggaacttat tgaaatagct
     5881 aaaataatgc aagctgttga gaaatagaaa taggccgaat ttaagtctgg tttgtaaatt
     5941 gcagagaatt tactcctgat gcttgcaatt atattgaata taagatagct cagaataaat
     6001 ccaaaaaggc aaattttatt cagcttttcg ggtaaaataa ataaaatgtt atcagcttta
     6061 aacccgccta aagaaaacca ctttaatgaa aatatatata ataatgccaa agtccaaaac
     6121 cccgcattgt aataggtttc cttttgtttc caatcgaatt tataattcag gtgcagttcg
     6181 gagcgaaaaa atattaccgt aatgggaata atgagcagaa atataaccgc cgattctttg
     6241 gcaagaaatg caaaagtcag gcacaatatt gttaataatg cacgccatat ctttttagag
     6301 tcgtaatatc ttattgcctg tatcgtagca ataagtgata aaatagtaca tatcagttct
     6361 tcgcggtttt ttaagctgag tacaacctct gtatgaaccg ggtgcaccac gaaaattatt
     6421 gcggatatta atgccagcag gttgccatat ggtgttttgt taaacaatag agcgattgta
     6481 taatatagaa ggcagcatgt tataatatag tatatcacat tgataatgtg accaacatga
     6541 ggaaagttat cagctccaac gcccacaaaa ttcacaaaga aatcccactc tattgcatat
     6601 atggcaacgg tcaacggcct gtacccaaaa tacaaattat cctgtgtgtg gtagcggctg
     6661 gtaaatattt ccggaatagc tttaattcct tgttgtgcca gttggtgatt ttcaatcaca
     6721 tagttgtcgt cgatgttgta gccattctga aacgactttg cataaacaat cagaataaga
     6781 acagagagaa aaaagaatac ccattttctc tttaaaaaaa acttcgcttt ttccgctttt
     6841 ttacccacgc ttttttgtgt aaaaataaac aaaattgtga attatttaat ggttattaca
     6901 aaaattgtgt ctctttaatg tttgctaaat atcagtaaat caatgaaatg ggataaaagt
     6961 ttaaaaagat aaaaaaaaga tataaaaaaa gttgtaaaga agtaaaaatg aatattatct
     7021 ttgcc
//
LOCUS       DAOOQP010000141         6979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_13282_length_6979_cov_4.463460, whole genome shotgun sequence.
ACCESSION   DAOOQP010000141 DAOOQP010000000
VERSION     DAOOQP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13282_length_6979_cov_4.463460"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLK75_11555"
     CDS             complement(<1..408)
                     /locus_tag="PLK75_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39922.1"
                     /translation="MRKNLLIAIILSIFTISSVIIYDKYWDDWTSVPESPKSPPPATL
                     YGIPASDYKIIVDSVQSGETIGSILAKYGISGNLIHKIGMMSTDSINLTMIHIGQKYT
                     VFIDTATDSIPITKYFIYENGLIVYTKYDFSIAD"
     gene            complement(408..3191)
                     /gene="leuS"
                     /locus_tag="PLK75_11560"
     CDS             complement(408..3191)
                     /gene="leuS"
                     /locus_tag="PLK75_11560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOY39923.1"
                     /translation="MEYSFKEIEEKWQANWREKEIYKVQINPRKPKFYVLDMFPYPSG
                     SGLHVGHPLGYIASDIYSRYKRNMGFNVLHPMGYDAYGLPAEQYAIQTGTHPAITTEK
                     NITRYRQQLDRIGFSFDWNREVRTCDPKYYKWTQWTFIKLFNHYYDINANKALPIEKL
                     IAVFEKSGSASVSAVHNKHDSFTPVEWQKMDDATKHDILMKYRLAYLSYTFVNWCPKL
                     GTVLANDEVVNGLSVRGGHPVERKLMKQWSLRITAYAQRLLDGLNTIDWPEAVKDIQS
                     NWIGRSEGATCYFSIKGSPNKVEIFTTRPDTMFGASFMVLAPEHELVGELTTPDKSKE
                     VAEYVHWAKNRPDVDRMADKKVSGVFTGSYAINPMNGNEIPVYIADYVLIGYGTGAIM
                     AVPAHDSRDFAFARHFNLPVVQVVSLEKATESDTSEWIDSYDSKEGYMVNSGFITGLP
                     VGDAIRATIEYLEKESIGTRQVNFRLRDAIFSRQRYWGEPFPVYYKNGIAYSFDASDL
                     PLVLPEIDAFLPTETGEPPLARAANWKTKDGYPIETSTMPGFAGSSGYYLRYMNPDNE
                     NEYFSQEAVQYWKQVDLYIGGREHATGHLMYARFWNMFLYDLGMVVYPEPFAKLINQG
                     MIQGRSNFVYRVTGSNEFVSFNLRNQYETQKIHVDVNIVSNDVLDIEAFKKWQPEFAN
                     AGFILEDGKYICGWEIEKMSKSLYNVQNPDDLIEKYGADTLRLYEMFLGPIEQAKPWD
                     TNGIEGVFRFIKKLWRLYHNEKNEFEVSNEQATTAELKTIHTAIKKVTQDIERFSFNT
                     AVSAFMICVNDLGRCNKKEVLEPLACIVSPFAPHIAEELWHLMGHNTSIIDAAWPQFD
                     ESLLQEDSITYPVSFNGKTRFQIDLPVIATKEEIEKSVLVDERTTKWVAEAEIVKIIV
                     VPHKIINIVIKQN"
     gene            complement(3283..>6979)
                     /locus_tag="PLK75_11565"
     CDS             complement(3283..>6979)
                     /locus_tag="PLK75_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY39924.1"
                     /translation="PVITSATGYCVADAPIDLTASVPNGTWSGPGITNSATGTFNPAI
                     AGVGNFTILYTLVSGACVAEDDAVLEVAEWPDATITPVGPLCETEDPVQLIAVTAGGL
                     WSGTGITNQTEGVFNPAYAGDGIHQIVYTTENAYCTDSDTIWIEVFNYNSVNITNIGP
                     FCQSAGTVTLSAIPSGGTWSGTGISNPVTGAFSVGGLLPGSYAITYTVINGVCIAEDT
                     QDIVVDEMVDATISGPVTFCETDPQTQFTTVTTGGTWSGPGVVSTTNGTFNPSVAGVG
                     VHSINYLVTNGSCNDSDTHTITVYDNPEPIISGPTSTCVNAGIIDFSVNLTGGTWSGI
                     GITDVNNGIFDPLVAGTGTHQIAFTIDNFNCQAVTYHNITVNTATPLSFLITDTTFCI
                     YETPVNITVNPAGGILSGAGISGTSFNPASAGPGPHVITYTFTNSYGCTSTITKVFTV
                     YNTPNASFTGLEPDYCLTDNPSILTGNPAGGVFFGDGITAGIFNPYTAGSGIHEIQYI
                     YIDSHLCSDTAQVSTTVYENPSVITDSLIEPSCFGSTDGGVYISVVSGDSPYAYLWNN
                     PSHSSTQDLIGIQAGNYTVSITDSHGCSTMSSITLNQPAALSLSPIVITNVSCFGGEN
                     GSANVSVTGGTPPYEYLWNSPNGGTTEFVNTLPAGTWIVLVSDANGCQANTSVTISQP
                     QNITMQVDEIENVLCFNGNNGSVSISVTGGSFPYTYQWDDPASSESQHVYNLPAGEYH
                     VTITDANFCEFTQSISITQPDSIILIPQIQHVSCGNTPGSVNVNVTGGTTPYEYIWNT
                     GSELPVISGLAAGTYTLSLTDAHSCTAEASYRIETTGTNQVNINQTASIHCFGDTTAS
                     LLGFMPEGAAPFEFQWSTGDSENEIANLGAGEYFLTITDSWGCTGESSRIVTQPEQLL
                     YAVVTTPVLCYGQSTGTASLNISGGVPPYLAVWENGHVGFSITGLSAGSYTVSVSDSY
                     NCQHIADFEISQPANPLSMVLNITPISCYQDEDGEINAVAQGGTPPYTYYWFAGNHFY
                     NTSTIRFLPQGVYNITITDSHSCSIDSLLFLPQPRPLDASFTTHDPSCVGNYDGAIYV
                     AVTGGTTPYVFETNGLTYEESPVDSLYQGTYSVVIRDAHDCTFELNGITLTDTYADCL
                     IIPNAFTPNGDGVNDEWILGNIHLYPKAYYKIINRWGQVIYESMANESSWDGTCNSQP
                     VPAGPYMYVINVQTQADPYIGIVTVVR"
BASE COUNT         1960 a         1487 c         1477 g         2055 t
ORIGIN      
        1 gtcggctata ctaaagtcgt acttggtgta cactatcaac ccattttcgt aaataaagta
       61 ttttgtaatg ggtatgctat cggttgcagt atcaataaaa actgtgtatt tctgaccaat
      121 atgaatcata gttaaattta tactatcagt cgacatcatc ccgattttat gaatcaggtt
      181 tccagagatg ccatactttg ctaaaataga tccaatcgtt tcacctgact gtacagagtc
      241 aacgataatt ttataatcag aagcaggtat gccatatagt gttgcaggtg gcggactttt
      301 gggtgattct ggtacagatg tccaatcatc ccaatattta tcataaatta taacagacga
      361 aatggtaaaa atcgaaagaa tgatggctat cagcaggttt ttcctcatca gttttgtttg
      421 atgacaatat taataatttt atgaggaacc acaattattt taacaatctc tgcttcggca
      481 acccatttag ttgttctttc atcaacaagg acgctttttt caatttcttc tttggtagcg
      541 ataacaggta aatcaatttg aaacctggtt tttccattga atgataccgg atacgtgatt
      601 gaatcttcct gcaaaaggct ttcatcaaat tgaggccatg ctgcatcaat aatactggtg
      661 ttatggccca tcaaatgcca aagctcctcc gcaatatggg gagcgaaagg agaaactata
      721 caagccaggg gttccaacac ctcctttttg ttacatctgc caaggtcgtt tacacaaatc
      781 ataaaagcac taactgctgt attaaacgaa aacctttcga tatcctgggt gacttttttt
      841 attgctgtat gaatggtttt taattcagct gttgttgctt gttcattgct gacctcaaat
      901 tcgttttttt cgttatgata aaggcgccac aattttttga taaaccggaa aaccccttcg
      961 atcccatttg tatcccatgg ctttgcctgc tctattggtc cgagaaacat ttcgtacagt
     1021 cggagtgtgt ctgcaccgta tttctcgata agatcatcag gattttgcac attataaagg
     1081 gatttcgaca ttttttcgat ctcccatccg cagatatatt tcccgtcttc gagaataaaa
     1141 ccggcgttag caaactcggg ctgccacttt ttgaaagctt caatgtcgag aacatcgtta
     1201 cttacaatat tcacatcaac atgtattttt tgagtttcat actggtttct taagttgaac
     1261 gaaacaaatt catttgaacc cgtaacccgg taaacaaaat tcgaacgacc ttgaatcatg
     1321 ccctggttga ttagttttgc aaaaggttca ggatatacaa ccatgccaag atcgtaaagg
     1381 aacatattcc agaaacgagc atacataagg tgcccggttg catgctccct gcccccgatg
     1441 taaaggtcaa cctgcttcca gtactgcact gcctcttgcg agaagtattc attttcgtta
     1501 tcaggattca tataccgaag ataatacccc gatgacccgg caaaccccgg catagttgat
     1561 gtctcaatag gatatccatc tttcgttttc cagttggctg cgcgtgcaag gggaggttct
     1621 ccagtttctg tcggaaggaa ggcgtctatt tccggcaata ctaaaggtaa atccgatgca
     1681 tcgaatgagt aggcaatccc atttttataa tacactggga aaggctctcc ccaatagcgt
     1741 tggcgactaa atatggcatc acgtaaccgg aaattaacct gacgtgttcc aatactttct
     1801 ttttcgagat attcaatagt tgcacgtatt gcatcgccaa cgggcagtcc ggttataaaa
     1861 ccggagttta ccatgtagcc ctcttttgag tcgtaagaat cgatccattc agaagtgtcg
     1921 gattccgtag ctttttcaag agaaacaacc tgaacaaccg gcaagttgaa gtgtcgtgca
     1981 aatgcaaaat cacgactatc gtgtgccggc acagccataa ttgctccggt tccataacca
     2041 attaaaacat aatcggcaat ataaactggt atttcatttc cgttcattgg gttaatggca
     2101 tacgacccgg taaacacccc tgaaactttc ttatctgcca ttctgtcgac atcaggccgg
     2161 ttttttgccc aatgaacata ttcggcaact tctttgcttt tatcgggtgt ggtgagttct
     2221 ccgaccaatt cgtgttcagg agcgagaacc ataaacgaag ctccaaacat agtatcaggt
     2281 cttgtcgtaa agatttccac cttatttggg ctccctttta tagaaaaata gcaggtggct
     2341 ccttcgctac gacctatcca attagattga atatctttta ctgcttcggg ccaatcgatt
     2401 gtattcaatc catccagtaa tcgctgggca taggcggtaa tacgaagtga ccattgcttc
     2461 attagctttc gttcaaccgg atgaccgcct cttaccgaaa ggccgtttac cacctcgtca
     2521 tttgcaagaa cagtacccag ctttggacac cagttcacaa aagtatacga cagataggca
     2581 aggcgatatt tcataagaat atcatgtttc gttgcatcat ccattttctg ccattcgacc
     2641 ggggtaaatg aatcgtgttt gttgtgcact gctgaaacag atgccgaccc agatttttca
     2701 aaaacagcta tcagtttttc aatgggtagg gctttattgg cattaatatc gtaataatga
     2761 ttgaacaatt taataaacgt ccactgagtc catttataat actttggatc acaggttctt
     2821 acttcacgat tccagtcgaa tgaaaaccca atacgatcga gttgctgccg atagcgggtt
     2881 atgttttttt cggttgtaat tgcagggtga gtaccggttt gaattgcata ttgttctgct
     2941 ggtaatccat aggcatcgta acccatagga tgaagcacat tgaaacccat attgcgttta
     3001 taacggctgt agatatccga tgcaatataa ccaagaggat gccctacgtg caaacccgaa
     3061 cccgatggat atgggaacat gtcgagcacg taaaattttg gttttcgggg gttaatctgt
     3121 accttatata tttctttttc acgccagttc gcctgccatt tttcttcgat ttctttgaaa
     3181 gaatattcca tattaaaaca atatggcggc gaatttacta aaaatcaccg aaaatcatgg
     3241 aatttcaaaa cagaatgaat ggttgaattt gcgaagtatt tatcatcgta ccactgttac
     3301 aatccctata tacggatctg cttgagtttg cacgtttata acgtacatat atggaccggc
     3361 cggtaccggt tgagaattac aggttccatc ccacgaactt tcatttgcca tactttcgta
     3421 tattacctga ccccagcggt taattatttt atagtaggct ttaggataca gatgaatgtt
     3481 cccaagtatc cactcatcat ttactccatc tccatttgga gtaaaagcat tggggataat
     3541 cagacaatca gcgtaggtgt cggtgagggt tatcccattc agctcgaaag tacaatcatg
     3601 agcatctctg ataacaaccg aataggtccc ctgatatagg ctgtcaacgg gtgattcttc
     3661 ataagtaagc ccattggttt cgaatacata aggggtagtt ccaccggtta cagctacata
     3721 tattgcccca tcgtaatttc caacgcatga tggatcgtgg gttgtgaaag aagcatccag
     3781 cggtctgggc tgtggaagga ataacaaaga atcgatggaa caggaatgag aatccgttat
     3841 ggtgatattg taaactcctt gtggtagaaa tcgtatagtt gatgtgttgt agaaatgatt
     3901 cccggcaaac caataatatg tatagggtgg tgtgcctccc tgagcaacag cgttgatttc
     3961 gccatcttcg tcctgatagc aacttattgg agtaatattc agtaccatcg acagggggtt
     4021 tgccggttgg gagatttcaa aatcagcaat atgctgacaa ttgtaggaat cggatacgct
     4081 taccgtataa ctaccggcag aaagaccggt aatactgaaa ccaacatgac cgttttccca
     4141 aaccgcaaga tagggtggaa caccgcctga aatgttcagg ctggcagtac ctgtagattg
     4201 cccgtagcaa agaacaggag ttgtaacaac agcatacaac aattgctctg gttgggttac
     4261 aattctggaa gattctcccg tacagcccca cgaatcagtt attgttagga aatactctcc
     4321 ggctccgaga tttgcaattt cattttcaga atcaccagtt gaccactgaa attcaaatgg
     4381 agcagctcct tccggcataa accctaatag cgaggctgta gtatcgccaa agcaatgtat
     4441 actagctgtc tgattaatgt tcacctgatt tgtgccagtt gtttcaattc tgtaggaagc
     4501 ttcggcggtg cagctgtggg catcggtaag gcttaaagta taagtacctg ctgcaagtcc
     4561 tgaaattaca ggtaattcag agcccgtatt ccagatgtat tcgtaaggtg tagtgccacc
     4621 tgttacattt acattcacag aaccgggagt gttgccgcat gaaacgtgct gtatttgagg
     4681 gattagaata atagagtcag gttgggtaat gctaatagac tgggtaaatt cacagaagtt
     4741 tgcatcagtt atggtaacat gatattctcc ggccggcagg ttataaacgt gctgcgattc
     4801 tgaagatgca ggatcatccc attgataggt atagggaaaa gagcctcctg taacgcttat
     4861 tgaaacagac ccgttattac cattaaagca caacacgttc tcaatttcat caacctgcat
     4921 agttatattt tgtggttgag aaatggtaac actggtattt gcctgacaac cattggcgtc
     4981 gcttacaagc acaatccatg ttcctgcagg tagcgtgttt acaaactctg ttgtgccacc
     5041 gtttggagaa ttccacagat attcgtatgg aggagtgcct cctgtaacac ttacattagc
     5101 gcttccattt tctccaccaa agcagcttac attggttatg actatcggac tcaacgataa
     5161 tgcggccggt tggtttaaag tgatactgct catggtactg caaccgtggg aatcggttat
     5221 agaaacagta taattaccgg cttgtatgcc aatcaggtct tgtgtagaag agtgcgatgg
     5281 attattccat aaataggcat atggcgaatc gcccgaaaca acgcttatat aaactcctcc
     5341 gtcggtgctg ccgaaacacg atggctcaat caggctgtcc gtaataacag aaggattttc
     5401 ataaacggtg gtagatactt gggcagtgtc actacataag tgagaatcaa tataaatata
     5461 ctgtatttca tggattcctg accctgcagt gtaagggtta aaaatgcctg cagttattcc
     5521 atctccaaag aacacccctc cggctgggtt cccggtcagg attgacgggt tgtctgttaa
     5581 gcagtaatct ggttccaggc ctgtgaaact tgcatttgga gtgttataaa ctgtaaatac
     5641 ttttgttatt gtgctggtgc atccgtagga gtttgtaaat gtataagtga ttacatgtgg
     5701 tccgggccct gctgaagcag ggttgaaaga tgttcccgat attccggcac cgcttaatat
     5761 accacccgct gggttaaccg taatgtttac gggtgtttca taaatgcaaa atgtagtatc
     5821 ggtgataaga aatgacaagg gtgttgctgt gtttactgta atgttatgat atgtaacagc
     5881 ctgacaatta aaattatcaa tagtaaaagc aatttgatgt gttcctgttc ctgcgacaag
     5941 tggatcaaat ataccgttat taacgtcggt aataccgatc cctgaccatg ttccacccgt
     6001 taagtttacc gaaaaatcaa ttattcctgc gtttacacag gtcgacgtcg gacctgatat
     6061 aattggctct gggttatcgt aaacagtaat ggtatgggta tcggaatcgt tacaacttcc
     6121 attggttacc aaataattaa tactatgtac tcccacacct gcaaccgatg gattaaatgt
     6181 cccgttggtt gtactaacta ccccgggtcc gctccaggtg ccccctgtag ttactgttgt
     6241 gaattgggtc tgtggatctg tttcacaaaa tgtaactggg ccggaaattg ttgcatctac
     6301 catttcatcc acaacaatgt cttgtgtatc ttcagcaatg cagacaccgt taataactgt
     6361 ataagtgatt gcgtaagatc caggaagcaa tccgccaaca gaaaaagctc ctgttactgg
     6421 gttgcttata ccggttccgc tccaggttcc ccctgaaggt atagcagata gggtaaccgt
     6481 ccctgcactt tgacagaatg gaccaatatt ggttatgtta acggaattgt aattaaaaac
     6541 ttcgatccaa atggtgtcgg aatcggtaca ataggcgttt tcagttgtat aaactatctg
     6601 atgaatccca tccccggcat aagccgggtt gaaaacccct tctgtttggt ttgtaatacc
     6661 cgtgccagac catagtcccc cggcagtaac agcgataagt tgaactggat cttcggtttc
     6721 acagagtggg ccaactggag taatagttgc atcaggccac tccgccactt ccaatactgc
     6781 atcatcttca gccacgcagg ctcctgaaac aagggtgtat aaaatcgtaa aattaccaac
     6841 tcctgctatt gcagggttga atgttcccgt tgcagaattc gtaatccccg gtccgctcca
     6901 tgtcccgttt ggaacagaag ctgtaagatc tataggagcg tcagcaacgc aatagcctgt
     6961 ggcactggta ataacgggg
//
LOCUS       DAOOQP010000142         6836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_13606_length_6836_cov_5.403038, whole genome shotgun sequence.
ACCESSION   DAOOQP010000142 DAOOQP010000000
VERSION     DAOOQP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13606_length_6836_cov_5.403038"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLK75_11570"
     CDS             complement(<1..332)
                     /locus_tag="PLK75_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY39925.1"
                     /translation="MPKQNTSSNLLVAKVLKANQRFNLIGNGDSVLAAISGGSDSLLM
                     LEALYRLLRSRKLDFQLSALHVSTENVDYSIDREKLDEFCHIRNIPVFFRSFRFEPSK
                     QTEKSPCF"
     gene            complement(418..1539)
                     /gene="gcvT"
                     /locus_tag="PLK75_11575"
     CDS             complement(418..1539)
                     /gene="gcvT"
                     /locus_tag="PLK75_11575"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOY39926.1"
                     /translation="MKRTAFTHFHEALGAKMSPFCGFNMPIEYTGINDEHITCREKLG
                     VFDVSHMGEFWVKGPKAAAFIQKVTTNDVYALTDGKVQYSCFPNEQGGIIDDLLVYRF
                     KADSYMLVVNAANVDKDWVWCLKVAREMGLTIGDELKNISDDICQLAVQGPYALKAMQ
                     KLTDANVIDMEYYTVQTCKFAGIDEVIFATTGYTGAGGCEIYAYNKDADKLWNAVMEA
                     GAEYGIKPIGLGARDTLRLEMGFCLYGHDINDETSPIEAGLGWITKFVDGNNFTMRPY
                     HEKLKNEGVIKRLCGFEMIDRGIPRQHYHVYDAQGNLIGEVTSGTISPMMKIGIGMAY
                     LNKGFWKEGTEIFIDIRDKHLKAKVVKMPMFKGQIPMVK"
     gene            1709..1831
                     /locus_tag="PLK75_11580"
     CDS             1709..1831
                     /locus_tag="PLK75_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39927.1"
                     /translation="MKFKLRTLTTADAECAYRAANNKNISNYMSDSVPGGIEKW"
     gene            1865..2128
                     /locus_tag="PLK75_11585"
     CDS             1865..2128
                     /locus_tag="PLK75_11585"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOY39928.1"
                     /translation="MKYFAIEIGGRLAGSIGLHIQTNNDIKNAEMGYWLAEVYHGKGI
                     VTDAVKAITAMAFGQFQIGRIFATPFSNNPASIKVLEKAGYIY"
     gene            2317..2958
                     /locus_tag="PLK75_11590"
     CDS             2317..2958
                     /locus_tag="PLK75_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39929.1"
                     /translation="MLGDVLLITDKHRVAAQELVALIMKSYHPKMVMAISGESGSGKS
                     ELAHCIGKSLVKEHAIRTKIIHSDNYYRIHPHQRTEWRKQHGFGAVGMHEIDWETLNK
                     NIDDFRHSRISEMPCIDIVPDEPDRLITDFSKIDFLIIDGLYALNAKGIDIGVFIDLT
                     YHETKKAQTDRGKEPANDFRFAVLEREHQEVITLKKMAHYIIQKDYTVVEKAL"
     gene            2972..4861
                     /locus_tag="PLK75_11595"
     CDS             2972..4861
                     /locus_tag="PLK75_11595"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39930.1"
                     /translation="MNRLHQITVFVLLIIIVGCKGNKTSDNYTYDKIPELKAPIVLYP
                     NQNTIYTQNYITDLSILDSVQFSMPKYFSISPDLQEIYLDIDFDSIKPLTELKFWIEG
                     YCYSVLCKKAPLTKATFTTEVIKNNTFNKVTLTGDFNNWNTDELPMIYQGYSWSHYLY
                     LEPGRYEYRIVGDGKYNLSSMKVKDSVKNEAGGYNYIKKIPDKSDKAPSISIKSIEED
                     EICITSTQVLVEYFIFWQNMRLPLDLKKVEGYNYYFSTPPVAEKINTSHIRVYGYNKN
                     GISNELVIPLVHGKAVNTATALNSSFPHSNIMLASDMVEPSVEILDSMISKHIFSKLG
                     INSLCISELPYTNDYIPTKSLGKTGNKTSSLMPFIEMRKSDKNTTSFEKLNQECTKSG
                     ITAYYVKNFGETYFNRKLNYIANSIFANDTLSFDGLAQAMRYSSRTFGDLHLLGNLVE
                     DPLTQGFHGQIYNIGSDSAIATAQQRGEYINKKWMQYIAFQFTIPGIPCLLLGSKTES
                     LCSALNDIEQSNLSPTEKEQLLVFLKLLQIRKNNIALFYGEIKILECNKSYLIYQRKY
                     LNNIVIVAFNKTPFTQMLGVNLTDIQSSTRFSNHFASLIKQEDFYVRMEIPAYSFEIL
                     TSKNK"
     gene            4954..6339
                     /locus_tag="PLK75_11600"
     CDS             4954..6339
                     /locus_tag="PLK75_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOY39931.1"
                     /translation="MKRLFFIITAALLIASCNQPLKKPETKMNEDKKALETFAPEWTN
                     NKNIYEVNIRNFTPEGTLNAFANHLPRLQAMGVDIIWLMPVNPIGEMNRKGSLGSYYS
                     IKDYTQINPDFGTLEDFRNLVKQAHSLGMYVIIDWVANHAAWDNTWTKNHPEYFIHDE
                     NGNFTPPVPDWSDVIDLNYDNPQMRQAMIDAMKFWVSETDIDGFRCDVAMMVPVDFWE
                     DARSQLDSIKSVFMLAEAEEPAHHFASFDASYSWELMHTFNDIAKGSKNSESIDSLLR
                     DEKVKFPAKAFRLRFITNHDENSWNGTEFQRLGDGVDAFTVLCYTLPGMPLVYSGQES
                     AFNRALRFFDKDQIDWGNYSKQKFFRALNHLKHEHECLWNGSYGGDMITVFNHQNNHN
                     IYAFLRCKKESIIGVIVNLSNQPEKVTLVIPDTYTGKYKDYFRGPEFEIGKEVDIAMQ
                     AWEYRVFEKVN"
     gene            complement(6358..>6836)
                     /locus_tag="PLK75_11605"
     CDS             complement(6358..>6836)
                     /locus_tag="PLK75_11605"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOY39932.1"
                     /translation="FNALRREYTYYICNGKTPFFSKYSWEYHQKLDLQLMNDAAGLLI
                     GKKNFGSFCKLHSNNKTNICEVYVAEWRCFGMFYVFTISADRFLRNMVRAIVGTLVDV
                     GRGRTSVDEFRTILAAENRCASGQSVPARGLFLTDVTYPDGDFIVHKGPSLPLLLL"
BASE COUNT         2193 a         1392 c         1341 g         1910 t
ORIGIN      
        1 taaaaacagg gtgatttttc ggtttgcttt gatggttcaa acctaaaact tcggaaaaat
       61 acaggaatgt tcctgatatg gcaaaattca tcaagtttct ctctatcgat gctataatct
      121 acattttcgg tagaaacatg caatgcgcta agctgaaaat ccagctttcg gctacgcaat
      181 aacctgtata acgcttcgag catcagcagc gaatcgcttc ctcccgaaat ggcagcaagc
      241 acactgtcgc cattcccaat caggttaaac cgctggttag cctttagcac tttagctaca
      301 agcagatttg atgatgtatt ctgtttcggc attgggtaaa aataacagaa aattgcgttt
      361 aaaacaagaa agccactgct gtaacaacaa tggctttcat aaatattaat tgtttgttta
      421 tttaaccata ggtatctgtc ctttaaacat tggcattttc accacttttg ccttcagatg
      481 tttatcgcgg atatcaataa aaatctcagt gccttctttc cagaatccct tattgagata
      541 ggccattccg attccgattt tcatcatcgg ggatatggtt cccgaagtaa cttcgccaat
      601 caggtttcct tgtgcatcgt acacatgata atgctgacgt ggaataccac ggtctatcat
      661 ttcaaacccg cagaggcgtt ttattacacc ttcatttttg agcttttcat gatacggacg
      721 catggtaaaa ttgtttccat caacaaattt ggttatccag cccaagcctg cttcgatggg
      781 tgaggtttca tcgttgatat cgtgtccata gaggcagaaa cccatttcga gacgcaaggt
      841 gtcgcgtgct cccaaaccaa ttggtttgat accatattca gcaccggctt ccataaccgc
      901 attccaaagt ttgtcagcat ctttgttgta tgcatatatt tcgcatcctc cggctcctgt
      961 atatccggta gtggcaaaaa tcacctcatc aatcccggca aatttgcagg tttgcacggt
     1021 gtagtattcc atatcaatca cattggcatc ggtaagtttt tgcatcgctt tcaaggcata
     1081 aggtccctgt acggctagct gacagatatc gtcgcttata ttttttaatt catcaccaat
     1141 agtaaggccc atttcacggg ctactttcag gcaccatacc cagtctttat caacattggc
     1201 agcattcact accaacatat aactatctgc tttgaaccga tatacaagaa ggtcatcgat
     1261 aattccaccc tgttcgttgg gaaagcaact gtattgcacc ttgccatcgg taagggcata
     1321 cacatcgttg gtggttactt tctgaataaa ggcagcagct ttcggtcctt tcacccaaaa
     1381 ctcacccata tgcgacacgt cgaaaacacc aagtttttcg cggcaggtaa tatgttcatc
     1441 gtttatgccg gtatattcaa tcggcatgtt aaagccacag aaaggcgaca tttttgcgcc
     1501 caatgcttcg tggaaatgtg taaatgcagt tcttttcatt actatggaat tttacgtttt
     1561 atattctaat ggcaacaaag gtaaaaatat aaaacaataa aaattatgac ctagggcagt
     1621 aaattatcaa ctattaacca atacaaaaac tcttattgta tcgtaaaatt cagtaatttt
     1681 tgcagattaa aatacagtac ttaaataaat gaaatttaaa ctcagaacgc taacaactgc
     1741 tgatgcagaa tgtgcttacc gtgctgccaa caataaaaac atttcgaatt acatgtcaga
     1801 cagcgttccc ggcggcattg agaaatggta aaaattcatt gagtttgcta atagcagtga
     1861 atcgataaaa tattttgcaa tcgaaattgg tgggaggctt gcaggtagta tcgggctgca
     1921 tattcaaaca aacaacgaca ttaaaaatgc tgaaatgggt tattggcttg ccgaagtgta
     1981 tcacggcaaa ggaattgtca cggatgcagt aaaagcaata acagcaatgg cgttcgggca
     2041 gtttcagatc ggacggatat ttgccacccc tttttcgaac aacccggcat cgattaaagt
     2101 tcttgaaaaa gcaggatata tttattaaaa ccatctccct gcagcagtta tcagaaatgg
     2161 gattgcaaca gacaaactgg ttgatggtat tacccgcaat atcaaatgaa gtttgcccga
     2221 tgccgatggc aataacggag aaaataaacc acatggcagt tcagtttgtt ttgttatgtt
     2281 gattttgttt cataaatttc gtgcaaaatt aggagcatgc tgggagatgt acttttaatc
     2341 actgataaac accgtgttgc agctcaggag cttgttgcgt taatcatgaa atcgtatcac
     2401 cccaaaatgg tgatggcaat ttcgggtgag tcgggcagcg gaaaatcgga gttggcacat
     2461 tgcattggca aaagcttagt aaaagaacat gccatccgaa ccaaaatcat tcattccgat
     2521 aattattacc gcattcaccc acatcagcgg accgaatggc gtaagcaaca cgggtttgga
     2581 gctgtaggca tgcatgagat cgattgggag accctgaata aaaatatcga tgacttcagg
     2641 cacagccgca tttcagaaat gccatgcatt gatattgtac ccgacgagcc tgacagactg
     2701 ataaccgatt tttcgaagat cgattttcta attatcgatg gtttatatgc cctcaatgca
     2761 aaaggtattg atattggtgt ttttatagac cttacgtatc acgaaacaaa aaaagcccag
     2821 accgaccgcg gaaaggagcc tgcaaacgac tttcggtttg ctgtgctgga gcgggaacat
     2881 caggaagtta ttacacttaa aaaaatggca cactacatta ttcagaaaga ttacactgtt
     2941 gttgaaaagg cattataatg aagggaaaca tatgaatcgt ttacatcaaa ttacagtatt
     3001 cgttttgctc ataattattg tgggctgtaa gggtaataaa accagcgaca actacacata
     3061 cgacaaaatt cccgagctga aagctccaat cgtgttgtat cccaatcaaa acacaatcta
     3121 cacccaaaac tatattaccg atttgtcaat actcgattct gtacagtttt cgatgcctaa
     3181 atattttagc atatcacctg atttgcaaga gatttacctc gatattgatt ttgattcaat
     3241 aaaacctttg actgagctta aattctggat tgaaggatat tgctattctg ttctctgcaa
     3301 aaaagcccca ctgacgaaag ctactttcac cactgaagtt attaaaaaca atactttcaa
     3361 taaagtaact ctgacaggag acttcaataa ctggaatact gatgaactcc ccatgatata
     3421 tcagggatac tcatggtcgc attacttgta tcttgaaccg gggcgttatg agtatcgcat
     3481 tgtgggtgac ggaaaataca atctttccag tatgaaggtg aaagattcgg tgaagaatga
     3541 ggcaggcggc tataattaca taaaaaagat tcctgataag agtgataaag caccctcgat
     3601 tagtattaaa tcaattgagg aggatgaaat atgcataact agcactcagg ttctggtcga
     3661 gtactttatt ttttggcaaa acatgaggtt gccgttggat ctaaaaaagg tggaaggcta
     3721 caattattat ttctcaactc cccctgtggc agaaaaaatc aatacttccc acattcgggt
     3781 ttacggttat aacaaaaatg gcatttcgaa cgaactggtg attccattgg tacacgggaa
     3841 agcagtaaac acagctacag cattgaacag ttcattccct cacagtaaca ttatgcttgc
     3901 atcggatatg gttgagccct cggttgaaat attggacagc atgatttcaa aacacatatt
     3961 tagtaaattg ggaattaatt ctttgtgcat cagcgaattg ccttacacaa acgattatat
     4021 accaacgaaa tcattaggca agaccggtaa caaaacaagc tcccttatgc catttattga
     4081 gatgagaaaa tcggataaaa atactacttc tttcgaaaag ctcaatcagg aatgcactaa
     4141 atcaggaatt acggcttact atgttaaaaa ttttggagaa acatatttca atagaaagtt
     4201 gaattatatt gcgaatagca tatttgccaa cgatactcta tcattcgatg gcctggcaca
     4261 ggcaatgaga tattcatcac gtacgtttgg cgacttgcat ttgcttggca accttgtaga
     4321 agacccactt acacaaggat tccatggtca gatttataat atcggatcgg attcggcaat
     4381 tgcaaccgcg cagcagcggg gagaatatat aaacaaaaaa tggatgcagt atattgcttt
     4441 ccagtttact attccgggaa ttccctgttt gttgttggga agtaaaacgg aaagcctatg
     4501 tagtgcatta aacgacatcg agcaatcgaa cctttcgcca acagaaaagg agcagctttt
     4561 ggtttttttg aaattactgc aaatccgcaa aaacaatatt gcattgtttt acggggaaat
     4621 aaaaattctg gaatgcaaca aatcgtacct gatttatcaa cgaaaatact taaataacat
     4681 tgtaattgtt gcgtttaaca aaaccccatt tacccagatg ctcggagtta accttactga
     4741 tattcagagt tcgacacggt tttccaacca ctttgcaagc cttataaaac aggaagattt
     4801 ttatgtaaga atggagatac ctgcctatag cttcgaaata ttaacctcaa agaataaata
     4861 gtaccatgaa gtcgagcatg ataataatat caccggccaa gatgaatatt aatctatgca
     4921 agattccctt agtccaataa aaaatattta caaatgaaaa ggttgttttt tattattact
     4981 gctgcactgc tgatagcttc gtgtaatcaa cctttaaaaa aaccagaaac taaaatgaat
     5041 gaagataaaa aagcactcga aacttttgcg ccggaatgga cgaataataa aaacatttac
     5101 gaagttaaca tccgcaactt cacacccgaa ggcaccctga atgcctttgc aaatcattta
     5161 ccgcgcttac aagcaatggg ggtggatata atatggctca tgccggttaa ccccattggc
     5221 gaaatgaacc gaaaaggcag cctgggcagc tactattcaa taaaggacta tacccaaatc
     5281 aacccggact ttggtactct cgaagatttt agaaaccttg taaaacaggc acacagcctg
     5341 ggtatgtatg tcattatcga ttgggttgca aaccatgctg catgggacaa cacatggaca
     5401 aaaaatcatc ctgaatattt tatacacgac gaaaacggta attttacacc acctgtaccc
     5461 gattggtcgg atgtaatcga cctgaactac gataatccac agatgcggca ggctatgatt
     5521 gatgccatga aattctgggt atcggaaact gatatcgacg gattccgttg cgacgttgca
     5581 atgatggttc cggttgactt ctgggaagac gcacgcagtc agctcgattc aataaaatcg
     5641 gtttttatgc ttgccgaagc cgaagagcca gcccatcatt ttgcatcatt cgatgcatct
     5701 tatagctggg aactgatgca tactttcaac gacattgcaa aaggcagtaa aaattcagaa
     5761 tcaatcgata gtctgctcag agatgaaaaa gtaaaattcc ctgcaaaagc cttccgtctg
     5821 agatttataa ccaaccacga cgaaaactca tggaatggaa ccgaatttca aaggctgggc
     5881 gatggcgttg acgcattcac cgtattgtgt tacacactgc ctggtatgcc tttggtttac
     5941 agtggtcagg agtcggcatt caacagagca ttacggtttt tcgataaaga tcagattgat
     6001 tggggtaatt attcgaaaca gaaatttttc agggcactta accatttgaa acacgagcat
     6061 gagtgtcttt ggaatggttc atatggagga gatatgatta ctgttttcaa tcatcagaac
     6121 aatcataaca tctatgcttt cttacgttgt aaaaaagaaa gcattatagg ggttattgta
     6181 aatctttcga atcaaccgga aaaagttaca ttggttatac ctgatacata caccggaaag
     6241 tataaggact atttcagggg cccggagttt gaaatcggga aagaagtaga tattgcaatg
     6301 caggcatggg aataccgtgt tttcgaaaaa gtgaactaag caccaatcac atacatatta
     6361 taacagcaac aggggaagag acggaccttt atggacaatg aaatcaccat ccgggtatgt
     6421 aacatcggta agaaataatc cgcgtgcagg taccgactgg cctgaggcac accgattttc
     6481 cgctgcaaga atcgtcctga actcatcaac acttgttcta ccccttccca catcgacaag
     6541 agtcccaaca atagccctaa ccatattcct taaaaaacga tctgcagaaa tagtaaatac
     6601 ataaaacatt ccgaaacatc gccattcagc aacgtaaact tcgcaaatat tggttttatt
     6661 gttgctatgc agtttgcaaa aactaccaaa attcttcttc ccaatcagta agccggctgc
     6721 atcattcatc agctgcagat cgagcttctg atgatactcc cacgaatatt tcgaaaagaa
     6781 aggagtttta ccgttgcata tataataggt atactcccgc cgcagggcat tgaaac
//
LOCUS       DAOOQP010000143         6562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_14318_length_6562_cov_4.138313, whole genome shotgun sequence.
ACCESSION   DAOOQP010000143 DAOOQP010000000
VERSION     DAOOQP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14318_length_6562_cov_4.138313"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="PLK75_11610"
     CDS             <1..1855
                     /locus_tag="PLK75_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39933.1"
                     /translation="NFYGAARLYEDALKFDNKMYDIHYKAAEAFRMDNDYVNAIKHYK
                     IVCDRAASRFPLSVLYLAEMQKSDEDYFSAQFNFNKYYTTHKSDSTDYFTIKAKQEIV
                     FCENAIKIKYNHTGVNIIALDTTINSLYSEIGASSWGDSVIFFSSVKPDSAFSNEFVS
                     SIYCSVRRNGEWVNTYRLDTIINATGYHNASAFFSTSTSTLLFSRKGFGNDDKTRIFK
                     TTYRNYEWETPVPLGENINDTSSNNTQATLAEFNGKTYLFFVSDRAKGLGGYDIWFSE
                     MKDDFICSPPKLAGIPTSVDSLYLAYFGVKSVINSAGNELCPYFNYKDSCLYFSSDWF
                     EGLGAYDIYKSKTNLIDWQEPVNLGYPINSSQNDLYYFIDNKSGFVMFTSNRKEARAL
                     KHQSCCNDIFMYPIDKEIDTVKIVEQQITTLTLEASELIPITLFFDNDHPNPNTWDTL
                     TTINYTDAYNRYVEKEELFCSEFSKGYRGDEKDLLRDSVLNFFDYQVSSEYQKLLKFA
                     ALLKELVLKDQQIIITIKGYTSPLNTTEYNEALAKRRISSLINFFREYDDGFFLPYEA
                     SGKIIIQRVAFGETLAGINVSDDPNDRRNSVFSPLASAERKIRIIAVSVNE"
     gene            1879..2991
                     /locus_tag="PLK75_11615"
     CDS             1879..2991
                     /locus_tag="PLK75_11615"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOY39934.1"
                     /translation="MIELFIEVIQQTLLITGFVITMMMIIEFVNILSHGNLLLRLKSS
                     PVGQVIAGTVLGMIPGCLGTYTAVSLFTHQLLSFGALVAALIATSGDETFVMLALIPR
                     EAIIIIAVTGVVGFIAGVLIDRFYKPTFNFYKNDYKFELHGHENSVAIWSKLSIKNFS
                     FTYRRVLALLIFGFFIIAPFTGIIEHNHNVMMPSIENVGNADALHNNDVGHPSHEHHV
                     NWLGITLAICASIGFIICLTCSEHFFTDHIWKHLIKKHFIKIFLWTFLALILIQVLLK
                     YTSFDIWISENLWLVLLAAVLIGIIPESGPHLLFISLYISGAIPLSILIANSIIQDGH
                     GALPLFAESKKSFFIAKGLNIVISLAIGAIGLLIGF"
     gene            3008..3439
                     /gene="ndk"
                     /locus_tag="PLK75_11620"
     CDS             3008..3439
                     /gene="ndk"
                     /locus_tag="PLK75_11620"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOY39935.1"
                     /translation="MERGEYTFTIIKPNALKNGHLSSILKAITNAGFRIVALKITIIN
                     KQQAERFYRVHKERDFFDMLTTFMSTGPIAVAIIKKQNAVEDYRKLIGNTDPAKAEEG
                     TIRKLFGMSITQNAVHGSDSESNAYHESCFFFNELEIFEQL"
     gene            3564..6233
                     /locus_tag="PLK75_11625"
     CDS             3564..6233
                     /locus_tag="PLK75_11625"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39936.1"
                     /translation="MKFCIKSISYKIVITIAATASLLLNGTVFAQNSYDVALSGIECP
                     NGQYMSDEALQYPYVIVENIGQETINSIDVYYRIDEGDAVLRHVAPIILNQGETRNVI
                     FPQITLPDGMHTFTAWVELDEGLSDENTENNTLSCSFIVAGGDIAILNIISPNNDICG
                     LDSIYPTVNIKNVGAIDVESFVVDFSMNEVIVQSITWTGSLTPNAIAQVVFDGVFPTN
                     GEYYIEFLAHSPNESVDANMVDNSAQTTLEMSAGNLIEVTIQTDQWGMQNSFEIINAV
                     GDAVLFDGPLATNSLLQYSICLPPGNYVFIIYDSNGNGMCIGPQSGYYIVENATTGEQ
                     IASDCDFQDQAEYPFTIIQPNSPPVASFNYDQLFNCSGYIQFTNTTVSDVPVLSYFWN
                     FGDGSTSGEESPIHVYAESGNYTVSLTSTNQFGSNTFSCSNCVPVNVNDGPEVFDGYS
                     CGPGNVSLSVQSSEGQVKWYHSQAELEAFHTGEAYLAEGLNETTVYYVEQITGNEHEY
                     FGMFDNTGPGGYFGWNIDRRLYFDAIDDVTIVSAKVYAQGTANRTFRLYDSNDELLDQ
                     KTVNIPAGESRIDLGFELPEGSGYAISVSMQNNLAYTGDYDGPSYPYPYVINGLISLT
                     GNNYSDSFYYFLYDIEVYAGFEDQCFSGRVPVTAYIQYPNPELPSVWLCCQGNECEIS
                     AAAGFETYHWSNGSYNQSVIVTQETSYSVTVTDEFGCPGSASTLMQFNEPGTIDVESH
                     VSSGPGVADGWAVVTVTGGTAPFQYVWSNGQTTPTATGLMQGTYSVTVIDAAGCELVA
                     EAVIIVGSSNLFVSDNISVYPNPCHNRIVVNTGNFNAKEFCIFDISGKLMILSDAPDQ
                     SFEIELNGLPSGLYIIRLKSHDNRYSKVTFIKD"
     gene            6249..>6562
                     /locus_tag="PLK75_11630"
     CDS             6249..>6562
                     /locus_tag="PLK75_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOY39937.1"
                     /translation="MPRYIIEFSFDGSGYCGWQFQPDSKTIQQTLQEALGMLLHANVE
                     LTGCGRTDTGVHALYYAAHFDCDADIQENTLTERLNRLLKQEFCVFSVQQVAEAFHAR
                     FNA"
BASE COUNT         1907 a         1228 c         1414 g         2013 t
ORIGIN      
        1 gaacttctat ggagccgccc gcctttatga agacgcactg aaattcgaca ataagatgta
       61 tgacattcat tataaagcag cagaagcgtt tcgaatggat aacgattatg ttaatgctat
      121 aaagcattat aaaattgtct gcgatagggc cgcatcacgc ttccctcttt cggttttata
      181 tttggcggag atgcagaaat cggatgaaga ttatttctca gctcagttta atttcaataa
      241 atactatacg actcacaaat cggactctac cgattatttt acaataaaag caaaacaaga
      301 aattgtgttt tgcgaaaatg caattaaaat aaaatacaat cacacagggg tgaatattat
      361 tgcacttgat actacaataa acagccttta cagcgaaatc ggggcttctt cctggggtga
      421 ttcagtgata tttttctctt cggtgaaacc cgattctgca ttttcgaatg aatttgtgtc
      481 atcgatctat tgcagtgtac gcagaaacgg agaatgggtg aatacttatc gactcgacac
      541 gataataaat gcgaccgggt atcacaatgc cagtgcgttt ttttctacct cgaccagcac
      601 gttgttgttt agccgaaaag gttttggtaa cgatgataag acgcgtattt ttaagacaac
      661 ctatcgtaat tatgaatggg aaactccggt tccacttgga gaaaatatca acgacacgtc
      721 ttcgaacaat actcaggcaa ctttggcaga gtttaatggt aaaacatact tgttttttgt
      781 ttctgacagg gcaaaagggc tcggggggta cgatatctgg ttttcggaaa tgaaagatga
      841 ttttatatgc tcaccgccga aattggccgg tattccaacc agtgtcgata gtttatatct
      901 tgcctatttc ggtgtaaaat cagtgattaa ctcggcgggt aatgaattat gcccgtattt
      961 taattataaa gatagctgcc tgtattttag ctctgactgg ttcgaaggtc tgggtgctta
     1021 cgatatttat aagtcaaaga ctaacctgat agattggcag gagcctgtaa atctgggtta
     1081 cccgattaac agcagtcaga acgacctgta ttattttatc gataataaat ctggttttgt
     1141 tatgttcaca agtaatcgca aagaggcccg tgccctcaag catcaaagtt gctgtaacga
     1201 catttttatg taccctatcg ataaagaaat tgatacagta aaaattgtgg aacagcagat
     1261 aacaaccctt actttagaag ccagtgagct gattccgatt accttgtttt tcgataatga
     1321 tcaccccaat ccgaacacat gggatacgct taccaccata aattatacag atgcctacaa
     1381 ccgatacgtt gaaaaggaag agcttttttg ctccgaattt tcgaagggtt atcgtggcga
     1441 tgaaaaagac ttgcttagag atagcgttct taactttttc gactatcagg ttagctcaga
     1501 gtatcagaaa ttgttgaaat ttgctgcatt actgaaagag cttgtgctta aggaccagca
     1561 gattattatt acaatcaaag gttataccag ccctttaaat acaacggaat acaatgaagc
     1621 tcttgcgaaa aggcgaatat caagcctgat taacttcttc cgggaatatg atgatggatt
     1681 ctttctgcct tatgaagcga gtggaaaaat aataatacaa cgggtagcgt tcggtgaaac
     1741 gcttgcaggg attaatgtta gcgatgaccc aaacgatcgc agaaattctg tttttagtcc
     1801 cctggcttcc gccgaaagaa aaattcgcat aattgctgta tcggtgaatg agtagattga
     1861 tgatttaata ttagtgaaat gattgaactt tttatcgagg ttattcagca gactttattg
     1921 attacgggtt ttgttatcac aatgatgatg ataatagaat tcgtaaatat tctttcgcat
     1981 ggtaacctac tgttaagact taaaagctca ccggttggcc aagtgattgc cggaacagtg
     2041 ttgggaatga ttccgggctg tctgggtact tatacggctg tttctctttt cacccaccag
     2101 ttgctaagct ttggtgcatt ggtggcagcg ctgattgcta ccagcggcga cgaaacattt
     2161 gtaatgttag cacttatacc acgcgaagcg ataataatca tagcagtaac cggtgtagta
     2221 ggttttattg caggggtgct gattgacaga ttttataagc ctacttttaa tttttataaa
     2281 aacgactaca agtttgagtt acacggtcat gaaaattcag tcgcaatctg gtcaaagctc
     2341 agtattaaaa actttagctt tacatatcgt cgcgtacttg cattacttat tttcggattc
     2401 tttatcattg caccttttac aggtatcatt gagcataacc acaatgtaat gatgccatcc
     2461 atcgaaaatg ttggtaatgc agatgcactg cataataacg acgttggaca tccttcacat
     2521 gagcatcatg taaactggct tggaataaca cttgccatat gcgcttcaat cggatttata
     2581 atatgtttaa cctgtagtga acatttcttt accgatcaca tatggaaaca tctcataaaa
     2641 aagcacttca ttaaaatctt tctctggacg tttttagcac ttatactaat tcaggtttta
     2701 ttgaaatata cttcctttga tatttggatt agcgaaaacc tctggctggt gttgctggct
     2761 gcagtactta ttggtattat acctgaatca ggaccgcatc tcttgtttat ttcattatat
     2821 atcagtgggg caattccctt atcgatttta attgctaaca gtataattca ggatggtcat
     2881 ggggcgttgc ctttgtttgc tgagtcgaaa aaaagctttt ttatcgctaa aggacttaac
     2941 attgtcatca gtctggccat aggtgctatt ggccttttaa ttggttttta atttaaaatc
     3001 ataatatatg gaaagaggag aatatacttt tacgattatt aaacccaatg ccctgaaaaa
     3061 tggtcatttg agcagtattc ttaaggctat cacaaacgca gggtttcgca ttgttgccct
     3121 taaaattact attattaaca agcagcaagc cgagaggttt taccgagttc acaaggaaag
     3181 agatttcttt gatatgctta ccacatttat gtcgacaggc cccattgcag tggctataat
     3241 taagaaacaa aatgcagtgg aagattaccg taagttgatt ggtaataccg accctgccaa
     3301 agctgaggaa ggaaccatca gaaaattgtt cgggatgtca attactcaaa atgctgtgca
     3361 cggatccgac agcgaaagca atgcttatca cgaaagctgc tttttcttta atgaactgga
     3421 gatttttgag caactgtaga aataatcttt gcataactca ggtattttta acacctgtga
     3481 attcatgctg ttttttgttg taaattgcgt ggctttcagg agaaacaaaa atgagcaaga
     3541 gttactattt aattcatatt tctatgaaat tctgtataaa atctatttcg tataaaatag
     3601 tcataacgat tgcggctact gcgtcgttat tattgaatgg gacagttttt gcacaaaatt
     3661 cttacgacgt agcactcagc ggtattgaat gcccaaacgg acagtatatg tccgatgaag
     3721 cattgcagta tccttatgta attgtcgaaa acatagggca ggagacgata aattcaattg
     3781 atgtgtatta ccgcatcgat gaaggtgacg ccgtattgcg gcacgttgca cctataatac
     3841 tgaatcaggg agaaaccaga aatgttattt tcccgcaaat aacactaccc gatggcatgc
     3901 atacttttac tgcatgggta gagctcgacg aagggcttag tgatgaaaac acagaaaata
     3961 atacgctttc atgtagcttt attgttgctg gcggcgacat tgctatcctg aatatcataa
     4021 gcccgaataa tgatatttgc gggttggata gtatttaccc cacggtaaac ataaaaaatg
     4081 tcggggctat agatgttgaa tcctttgttg tcgatttttc aatgaacgaa gttattgtac
     4141 aaagtataac ctggacgggt agccttactc ccaacgccat tgcgcaggtt gttttcgatg
     4201 gtgttttccc gacaaacggt gaatattata tcgagttttt agctcattct ccaaatgaaa
     4261 gcgtggatgc aaatatggtt gacaattcag cgcaaacaac gctggagatg tcagcaggca
     4321 atttgattga agttaccatt caaactgacc aatggggtat gcaaaattct ttcgaaatta
     4381 ttaacgccgt aggagacgct gttttatttg atgggccttt ggccaccaac agtttgctac
     4441 agtatagcat ttgcttacct cccggcaact atgtctttat tatttacgat agtaatggca
     4501 acggtatgtg tattggtccc caaagtggtt attacattgt tgaaaatgcc actaccggtg
     4561 agcagattgc cagtgattgc gattttcagg atcaggcaga ataccccttc acaattattc
     4621 aacccaatag cccacctgta gcttcgttca actacgatca attattcaat tgctccggat
     4681 atattcagtt cacaaataca acggtttcag atgttccggt tttgtcatat ttttggaatt
     4741 ttggcgacgg cagtacttcc ggcgaagaat cacccataca cgtttacgca gaaagcggta
     4801 attataccgt aagcctaact tcaacaaatc agttcggaag taatactttt tcatgttcca
     4861 actgtgttcc ggttaatgta aacgatggtc cggaggtttt tgatggatat agttgcggac
     4921 cggggaatgt aagcttatcg gtacaatctt ccgaaggcca ggtaaagtgg tatcattcac
     4981 aggctgaatt agaagcattt cataccggtg aagcatattt ggctgagggt ttaaatgaaa
     5041 ctactgttta ttatgttgaa cagattaccg gaaacgaaca cgaatacttt gggatgtttg
     5101 ataatactgg tcccggcggt tattttgggt ggaatattga ccgtaggttg tatttcgatg
     5161 ccattgatga tgtgacaatc gtgtcggcaa aagtatatgc tcagggtaca gcaaaccgta
     5221 catttcgttt atacgacagt aatgatgagc ttctcgatca gaaaaccgta aatatccccg
     5281 ccggtgaaag cagaatagac cttgggtttg aattacctga aggttcgggc tatgcaattt
     5341 ctgtttcgat gcagaataac ctggcttaca caggtgatta tgatggtccc agctacccat
     5401 atccttatgt gattaatggt ttgatttcgt taaccggaaa caattattcc gactcgtttt
     5461 actattttct ttacgatatc gaagtatatg ccggtttcga agaccagtgc ttcagtggcc
     5521 gtgttcctgt taccgcttac atacagtatc ctaacccgga actgccatca gtatggttat
     5581 gttgtcaggg caacgaatgt gaaatatccg ctgcagcggg attcgaaact tatcactggt
     5641 cgaatggcag ctacaatcaa agtgtgatag ttactcagga aacgtcgtat tctgttactg
     5701 ttaccgatga gtttggttgc cctgggtcgg cttcaacctt gatgcagttt aatgagcccg
     5761 gtactattga tgtggagtcg cacgtttcga gtggtcctgg tgtggcagat ggctgggcgg
     5821 ttgtaactgt taccggtgga acagcaccct ttcagtatgt gtggagcaat gggcaaacaa
     5881 cgcctaccgc taccggattg atgcagggaa cctattcagt tacggtaatt gatgcagccg
     5941 ggtgtgaact tgtggcagag gctgtgatta ttgtaggtag cagtaattta tttgtttctg
     6001 ataatatatc ggtatatccc aatccttgcc acaacagaat tgttgtgaat acagggaact
     6061 tcaatgcaaa ggaattttgc attttcgata ttagcggaaa attgatgatt ttatcagatg
     6121 caccggatca gtctttcgaa atcgaactta acggattgcc ttcaggcttg tatatcataa
     6181 ggcttaaatc gcacgacaat agatacagta aggtaacttt tattaaggat taatcacgtc
     6241 aattagaaat gccgcgctat attattgaat tttcgttcga cggcagcgga tactgcggtt
     6301 ggcagtttca acccgattca aaaaccattc agcaaacatt gcaggaagcc cttggaatgc
     6361 tgctccatgc taatgttgag cttacaggtt gcggcagaac cgacacgggg gtacatgcct
     6421 tgtattatgc cgctcacttt gactgtgatg ctgatataca agaaaatacc ctgactgagc
     6481 gacttaacag gttgctgaaa caggagtttt gtgtattttc agtccagcag gttgcggagg
     6541 catttcatgc gcgtttcaat gc
//
LOCUS       DAOOQP010000144         6552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_14347_length_6552_cov_4.691550, whole genome shotgun sequence.
ACCESSION   DAOOQP010000144 DAOOQP010000000
VERSION     DAOOQP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14347_length_6552_cov_4.691550"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3015)
                     /locus_tag="PLK75_11635"
     CDS             complement(<1..3015)
                     /locus_tag="PLK75_11635"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39938.1"
                     /translation="MYIPIKKTIALLVFSLFFIHGIFAQVTVDISSNVIEGCAPLEVA
                     FTSQVQNCTGTVNYTWDFDFPGGNSPLANPTTMFQNPGVYTIILQVSCAAGSASDMVQ
                     ITVHESPVAIISTTPQTGCRPMLVDFTDGSTPGDSDIIQWEWYFNDGTPFSSLQNPQH
                     LYEFTGIFNVLLTVRDENNCSSTTTAQNLVSVSDTPSIAFVAFPLSYCHPPLLVNFQS
                     NVVTNFNLPYTAIWDFGDGGTGSGQTTTHTYTTSNVFDVKLTAIDSYGCSNNRTYYDY
                     INITELVPEFNMLVGSIPIYDGSVICEGTTVHFSNLTPYPCQWDFGGGQTSQLNIVNY
                     SFQESGTYNITFTVMPPGGECENSVSFQLFVEEVSASFTTTPTSLFSCNTPFTVQFNS
                     TTSGNVVSFAYTFGDGSESSLASPSHTYETAGHFPVVLTATTSHGCTATATAEVVIES
                     PDATFVVDSTEGCAPVPVICTYTGTSGETITNYHWDFGDGFVIENGNPEEQHSFTNAG
                     EYDIVLTVTDESNCSASSTVTVNVGNPIPGELTFMIYDDLVSDWIIADELVYCAQDTF
                     RIYNSVWDNDDIDEFQWELDSIDDDNDEEYLEWAFDQDTGYVSVVFITNYNGCRDTLE
                     LDSIYFINGPVIESIQKEYDCDDPYNYLFTVNIINEEDDATLNWDWFIMQGETASVQL
                     FEELGSTEDQLSYTFPGSGVYWVKVLARSAETECDFVDSLRVDVSLPVASFIVSDDEF
                     CAGDEIYFDASGSSPDVQYFWVFGDGATQDWSENPITNHTYEGYGDFTVQLIVRDVNG
                     CEVETTRILHSRGPEVTIHVSPDPAHGCNSLTVHFSYDVVSDYPVSFNVWQISGSSQS
                     YYQDEFDKTFGPGTYDVSLSVTTTDGCDAQVTIPALVVVSSIEAEISTLDPVECVNVP
                     VQFYAGIENPLLNYSWNFGDGGISNQINPQHIYNTAGFYTATLVVDDGLGCNDEDEFI
                     IEVQGIDVDFTLEETVFTCYPAFLDIANNTDST"
     gene            3101..4105
                     /locus_tag="PLK75_11640"
     CDS             3101..4105
                     /locus_tag="PLK75_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY39939.1"
                     /translation="MRILALLICVLFSVIKLEAQDIHFSQYQYSPLTLNPAQTGNFNG
                     NWRFSNNYRDQWSAVGIPYQTISAGFDKPFRTKKGNIAIGVMFVNDRSGSSRLMTNKL
                     CLSLAYIVKLTDGQFIGIGAQGAYAVQAFSIGSLTFPVQWNELTGHYDTNLPNEVSQW
                     DENISYPDLSLGVHYRGESGNIKPFAGVSLYHINKPQISFLREDNRMDMRLTGYGGIT
                     IPLKSKVYIQPNLLCMYQKKASDYIAGIEIGYIFPEKSIIEQAYLGGGFRTSFSSYDA
                     AIVSGGIGLYHFLIGLSYDINVSRLATVSNMRGAFEISIIYKDITKTLHQITLPCDRY
                     "
     gene            4122..6215
                     /locus_tag="PLK75_11645"
     CDS             4122..6215
                     /locus_tag="PLK75_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY39940.1"
                     /translation="MFFAVLLPGMVVYAQDGNTKMKPYQIKNVARVAAESGDIFTAID
                     LYEAYAKERPGNTKVQHELGNLYWRERNYTKAAENLRKSFKGNRKKYIIDQYYYASCL
                     KIMGDYEESEKQFGIFLTHLKSKKYKDTYGKMAKLQIESFKKIPDLQKNADSIVVTHL
                     DTAVNKPHIEFSPIPYEGDKLIFASLKQEKLEYYDPVTQELPVRKFYVAYNKNGKWVN
                     LGEFDKNINGNNTNTGNGAFSESKKKFYFTRCEKNPGDNKVLCKIFVSINMNNEWQPP
                     TELNEEINMPLYTSTMPTLGVSKRNTDFLYYVSDRPDGRGGMDIWFTVFNDKKNIWET
                     PKNCGKAINTLGNEITPYYSIDTKTLYFSSDLHPGLGGYDIFGASGEGKQWDDAVNLG
                     ASINSSFDDLYYILTANRESGYFVSNRTGSNSIRHENCCDDIYEFVYKDFISISVTGQ
                     VFGITDSTFFKSIEEDYREDMQLNVDLNKSEEYIKVLFNYPVSLFRIDSQTGKEVFIK
                     TDSTRNGDYFFHLEQGVDYFIKVKDFNRIEKRLDFTTKNIVKADTLVLDAIIVNTIPS
                     QPFIVKNIYYEFGMSNLTGAAKSTIDKTIFSLMSKYPNIIVEISSHTDSVSSDDFNMN
                     LSQARAESVVKYLINKGISKERLVAKGYGEKFPIAPNSNPDGSDNPDGRAKNRRTEFR
                     IIGAVEDYSEVLYED"
     gene            complement(6212..>6552)
                     /locus_tag="PLK75_11650"
     CDS             complement(6212..>6552)
                     /locus_tag="PLK75_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY39941.1"
                     /translation="WVFTILKNQFVNRHRRNRQLRAILKSAEKRGLPFVYNSGNYAAE
                     ADSALMTDDVQNMINRLGPKYSGPLNDYINGYKYKEISDRFKLPIGTIKSRIFYARKM
                     IAEMRMNETS"
BASE COUNT         2090 a         1333 c         1313 g         1816 t
ORIGIN      
        1 agtggaatcg gtattattgg ctatatcaag aaaagcagga tagcaggtaa acactgtttc
       61 ttcgagtgta aaatcgacat caataccctg aacctcgata atgaattcat cttcatcgtt
      121 acagcctaaa ccatcgtcaa ccaccagagt ggcagtataa aatcctgctg tgttgtaaat
      181 atgttgaggg ttaatctgat tgctgatgcc gccatcacca aaattccagg aataattcag
      241 cagtgggttc tctatgccgg cataaaactg cacaggtacg tttacacatt ccaccgggtc
      301 gagcgtagaa atttctgctt ctatgcttga aaccactacc agtgcaggaa ttgttacctg
      361 agcgtcacat ccatcggtgg ttgtaacaga taatgacaca tcataagtac ccggaccaaa
      421 agttttatcg aattcatcct gataatacga ttgcgaagag ccacttatct gccagacatt
      481 aaaacttacc ggatagtcag aaactacatc ataggagaaa tgcacggtca agctattgca
      541 accatgggcc ggatcaggac tgacatgaat tgtaacttca gggccccggc tatgtaaaat
      601 cctggttgtt tcaacttcgc acccgttcac atcgcgaaca atcagttgaa cggtaaaatc
      661 accatagccc tcgtaagtat gatttgtgat tgggttttct gaccaatcct gtgtggctcc
      721 gtcaccaaaa acccaaaaat attgcacatc gggggaagac cctgatgcat cgaaataaat
      781 ttcgtcaccg gcacaaaact catcatccga cacaataaag cttgccacag gaaggctgac
      841 atccacccta agggagtcga caaaatcgca ttctgtttca gcactgcggg caagtacttt
      901 tacccaatat acaccggaac ccggaaatgt gtacgacaac tggtcttcgg tgcttcctag
      961 ctcttcaaaa agctggacag atgcagtttc accctgcata atgaaccaat cccagtttag
     1021 cgttgcatca tcttcctcat taataatatt tactgtaaac aaataattat atgggtcgtc
     1081 gcagtcgtac tccttctgaa tgctttcgat taccggacca ttaataaaat agattgaatc
     1141 cagttcgagt gtgtcgcggc atccattata attagttata aaaacaacag aaacatatcc
     1201 tgtgtcctga tcgaaagccc attcaagata ctcttcatcg ttgtcgtcgt caattgaatc
     1261 gagctcccac tgaaattcat cgatatcatc attatcccat accgaattat atattctaaa
     1321 agtatcctga gcacagtaga ctaactcatc tgcgataatc caatccgaaa cgagatcgtc
     1381 ataaatcata aaagttagtt ctccaggaat aggatttcca acattaactg taacagttga
     1441 acttgcagaa cagttacttt catcggtcac agtgagaaca atatcatatt caccagcatt
     1501 ggtgaacgaa tgttgttctt cgggattacc attttctatt acaaaaccat cgccaaagtc
     1561 ccagtgataa ttggtaatag tttcacctga ggtcccggta tacgtgcaaa taaccggtac
     1621 tggtgcgcac ccctcggtgg aatcaaccac aaaagtagca tcgggactct caatgacaac
     1681 ttctgctgtt gcagtagcag tgcaaccatg agatgttgtt gcggtaagaa ccacagggaa
     1741 gtggcctgct gtttcgtagg tatgcgacgg gcttgccagg cttgattcag aaccatcgcc
     1801 aaaggtatat gcaaagctaa ccacattacc gcttgtagtt gaattgaact gcacagtaaa
     1861 aggagtgttg catgaaaaca aagaggtagg tgttgttgta aatgaagcgc taacttcttc
     1921 gacaaacagc tgaaaactaa ctgaattttc gcattcaccg cctggaggca ttacagtaaa
     1981 agtaatgttg tatgttcccg attcttggaa tgaatagttc acaatattca attgcgacgt
     2041 ctgtccacca ccaaaatccc actgacaggg ataaggtgta aggttcgaaa agtgtacagt
     2101 agtaccttca caaataacgc taccatcata tatgggaatg ctgccaacga gcatattgaa
     2161 ttcaggaacc aattctgtga tattaatata atcataatac gttcggttgt tagaacatcc
     2221 gtaagaatca atggctgtaa gtttcacatc aaaaacattt gatgtagtat aggtatgagt
     2281 agtagtttgc cccgaacctg ttccaccatc tccaaagtcc cagatggctg tatatggtag
     2341 attaaaattt gtaaccacat tgctctggaa atttaccaat aatggagggt gacaataact
     2401 caatgggaaa gcaacaaaag caatgctggg tgtatcggaa accgaaacga gattttgtgc
     2461 tgtagtggtt gaggagcaat tgttttcatc tcttacagta agcaacacgt taaatatgcc
     2521 agtaaactca tataaatgct gcgggttttg caaagaagaa aaaggcgttc catcattgaa
     2581 ataccattcc cattgaatga tatcactgtc tcctggggta gatccatcgg taaaatcaac
     2641 caacattggc ctgcatccgg tctgtggtgt tgtggaaatg atggcaaccg gtgactcatg
     2701 aactgttatt tgcaccatat cggatgcaga accagcagca cacgaaacct ggagaattat
     2761 tgtatacact cctgggtttt gaaacatagt ggttggattc gccagaggac tgttgccacc
     2821 gggaaagtca aaatcccatg tataattaac cgttcctgtg caattttgca cctgacttgt
     2881 aaatgcaact tcaagcggag cacaaccttc tataacattg gaactgatat caacagtaac
     2941 ctgagcaaaa attccatgaa taaaaaacaa gctaaaaact aataaggcta ttgttttttt
     3001 tattggaata tacattgtag taatggttaa aataatggtt tttttattaa aacaaaaata
     3061 ctatatttga cgaataaaaa aagtaaaagt gattaattta atgagaatat tggcattgtt
     3121 gatatgtgtg ttgttttccg taataaaact ggaagcacag gatatacatt tcagtcagta
     3181 ccaatatagt cctcttacat taaatcctgc acaaacgggg aatttcaacg gcaactggcg
     3241 cttttcgaat aattatcgcg atcaatggag cgctgttggc ataccttatc aaaccatttc
     3301 agccggattc gataaaccct tccgcactaa aaaaggtaat attgctattg gtgtaatgtt
     3361 tgtaaacgac cgttcgggca gttcacgcct gatgacgaat aagctttgtc tttcgttggc
     3421 ctatatagtg aaattaaccg acgggcagtt catcggaatt ggcgctcaag gagcctatgc
     3481 ggttcaggca ttttcgattg gcagcctaac tttcccggta caatggaatg agcttaccgg
     3541 acattatgac accaatcttc cgaatgaggt cagccaatgg gatgaaaaca tcagctaccc
     3601 tgaccttagt ctgggtgtgc attatcgcgg tgaatcagga aacattaaac ccttcgcagg
     3661 ggtttcgctt tatcacatca acaagccgca aatatccttt cttcgtgaag ataaccggat
     3721 ggatatgagg ctaacaggtt acggaggaat tactattccc ttgaaaagca aagtgtacat
     3781 tcaacccaat ctgctgtgta tgtatcagaa aaaagcatcc gattatatag caggcatcga
     3841 aatcggatat attttccccg aaaagagcat aatcgagcaa gcctatcttg gaggaggttt
     3901 ccgcacttct ttcagttctt acgacgctgc cattgtttcg ggaggcatag gtttatacca
     3961 ctttctgata ggcttaagct acgacattaa cgtctctcgc ctagccactg ttagcaatat
     4021 gcgcggagct tttgaaattt ctatcatata taaagatatt acaaaaaccc tacatcagat
     4081 tacattgccc tgtgacagat actaagttta taaaatggct tatgtttttt gcagtattac
     4141 tgccaggtat ggtggtatat gctcaggatg gaaacaccaa aatgaagccc taccagataa
     4201 aaaatgttgc ccgtgttgcc gccgaatctg gcgatatatt tacagcaatc gatttatatg
     4261 aagcttacgc aaaagagcgg ccagggaaca caaaggttca gcacgaactc gggaacttgt
     4321 actggcgcga aagaaattac accaaagccg ctgaaaatct gagaaaatcg ttcaaaggca
     4381 acagaaagaa atatatcatt gatcagtatt attatgcaag ctgccttaaa ataatgggcg
     4441 attatgaaga atcggaaaaa caatttggga tattcctaac ccatcttaag tcgaaaaaat
     4501 ataaagacac atatggaaag atggctaagc tgcagattga aagttttaaa aagataccgg
     4561 atttacaaaa aaacgcggac agtattgtag ttacacacct tgatactgca gttaataaac
     4621 ctcatattga attctctccg attccttacg aaggagataa acttattttt gcttcattga
     4681 agcaggaaaa actggagtac tacgatccgg ttacacaaga attaccggtt cgcaagttct
     4741 atgttgccta taataaaaac ggcaaatggg ttaatctggg agagtttgac aaaaatatta
     4801 acggcaacaa cacaaataca ggcaatggag cattctccga aagtaaaaag aaattctatt
     4861 ttacacgttg cgaaaaaaat ccgggcgaca ataaggtttt gtgtaagatt ttcgtaagta
     4921 ttaacatgaa taacgaatgg cagcctccca cagagttaaa tgaagaaatc aatatgcctt
     4981 tatatacttc cactatgcca actctgggag tttcgaagcg aaacaccgac ttcctgtatt
     5041 atgtttcaga ccgaccagat ggcagaggtg gaatggatat atggttcact gtgtttaatg
     5101 ataaaaagaa tatttgggaa accccgaaga attgtggaaa ggcaatcaac acgctgggaa
     5161 atgaaataac tccttactat agtattgata caaaaacact ctattttagt tccgatttgc
     5221 atcctggtct tggcggctac gacatattcg gtgcatccgg tgaaggaaaa caatgggacg
     5281 acgctgttaa ccttggggca tcaatcaact catcatttga tgacctatat tatattttaa
     5341 cagccaatcg tgaaagcggg tactttgtat cgaaccgcac cggcagcaat tcaattcgac
     5401 atgagaattg ctgtgacgat atttatgaat ttgtttacaa agatttcatt agcatttcgg
     5461 ttaccggtca ggttttcggc atcaccgaca gcactttctt taaatcaatt gaagaggatt
     5521 accgcgaaga tatgcagctt aatgttgacc ttaataagtc ggaagaatac attaaagtcc
     5581 ttttcaatta ccccgtttca ctattcagaa tcgacagcca aacgggcaaa gaggttttca
     5641 ttaagactga cagtacccga aacggcgatt atttcttcca tcttgaacag ggtgttgatt
     5701 attttattaa agttaaagac tttaaccgca tagaaaagcg cctcgacttc acaacaaaaa
     5761 atattgtaaa agcagacaca ttggttttgg atgcaattat tgtaaacacc atcccttcgc
     5821 aaccatttat tgtaaagaac atttattatg aatttgggat gtcgaacctt accggggcag
     5881 ccaaatcgac tattgataaa acaattttca gtctaatgtc gaaatatccg aacataatcg
     5941 ttgaaatcag ttctcatacc gacagtgttt catcagatga tttcaacatg aatttatcgc
     6001 aggcgcgtgc tgaaagcgtt gttaaatatc ttattaataa aggtataagt aaagaacgtc
     6061 tggttgcaaa aggttacggc gaaaaattcc cgattgctcc gaattcaaac cctgatggta
     6121 gcgataatcc tgatggaaga gcaaagaaca ggagaacaga gtttaggatt atcggtgcag
     6181 ttgaagacta ctctgaagta ctatacgagg attaacttgt ttcattcatt cgcatttccg
     6241 ctatcatctt tcgtgcataa aatatacggc ttttaatagt tcctatcggc aatttgaagc
     6301 ggtcggaaat ctctttgtat ttatacccgt tgatataatc gtttaacggc ccactgtatt
     6361 tcgggccaag acgattaatc atattctgca catcgtcagt cattaatgct gagtcagcct
     6421 cagcggcata attaccgcta ttataaacaa agggcaggcc tcttttttca gctgatttaa
     6481 gaatggctcg caactgtcgg tttctgcgat gcctgttcac aaactgattt ttcagtatag
     6541 taaaaaccca gg
//
LOCUS       DAOOQP010000145         6294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_15096_length_6294_cov_5.370091, whole genome shotgun sequence.
ACCESSION   DAOOQP010000145 DAOOQP010000000
VERSION     DAOOQP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15096_length_6294_cov_5.370091"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..841)
                     /locus_tag="PLK75_11655"
     CDS             complement(62..841)
                     /locus_tag="PLK75_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOY39942.1"
                     /translation="MQALISIISDWKNHDYYLCTVKSAIIAAVPDVRFIDVTHDIPAY
                     NEQRAAFVLRSSYKAFPEGTVNLMCVNTEANSESPYVIIKNKGQYFIGTDTGIFGLVF
                     DNQPEMVIYIYPDSLPKTYTFPCLSIYSKLACYILGGGDITTLGEKVDDIKRKTPLAP
                     KITEGMIVGHILYIDSYSNGITNINRETFDKHVGSGKFDIYINSNKYHVDKICNSYNE
                     VEKGDLVVIFNSLDLLEVAIREEDAASLLSLDKKTLISIRF"
     gene            931..1893
                     /locus_tag="PLK75_11660"
     CDS             931..1893
                     /locus_tag="PLK75_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOY39943.1"
                     /translation="MTEKSFLLEMADPRQLCGLKDENLYLLQRFFAKLKIIVRGDSLK
                     VMGDEEEVARFESKLKILTAFINHYNKLSADDVQMLLQNDDQGETLNFGKEKDVLLYS
                     ITGKPIKARTKNQRALTEAAVENDLLFAVGPAGTGKTYTAIALAVKALKDHKVKRIIL
                     SRPAVEAGETLGFLPGDLREKLDPYLQPLYDALMDMIPSRKLEQMLEDKVIQIAPLGF
                     MRGRTLNNAFVILDEAQNATFNQMKMFLTRMGEYSKFIVTGDLTQIDLPNKKESGLLK
                     ASEILREIDGIKFIFFDADDVIRHRLVTSIINAYEKENNNNQYE"
     gene            1886..2836
                     /locus_tag="PLK75_11665"
     CDS             1886..2836
                     /locus_tag="PLK75_11665"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOY39944.1"
                     /translation="MNKTLTKTDFRFKNQSHVYHGKVRDVYFIDEEYIVMVVTDRISA
                     FDVILPKGIPFKGQVLNQIADKFLKATEDIVPNWRIATPDPMAMVGYKCQPFPVEMII
                     RGYLTGSSWRDYKNGARSICGVPIADGMKEHQAFEKPIITPTTKAEMGYHDENISKEQ
                     IIVSGMVSEEDYQMLERYTYELFRRGTEIAEQQGLILVDTKYEFGKRNGQIYLIDEIH
                     TPDSSRYFYKESYADNFKAGLPQRQLSKEFVREWLISSNFMGRQGEQIPQMTDEKASE
                     ISQRYIELYENITGDAFIPGTGENLSQRIDDNVTAFLRNL"
     gene            complement(2837..4921)
                     /locus_tag="PLK75_11670"
     CDS             complement(2837..4921)
                     /locus_tag="PLK75_11670"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOY39945.1"
                     /translation="MKKTIIYLFIITGMLACNIPANAQESSWRCATPEMNRKALEANP
                     AAINDILELRNFVEQYVEQEQKTDQVYVIPVVFHIVHNYGPENISKEQIEDQIRILNL
                     DFRKLNSDTMYIIDPFKEIAGDSHIEFRLAKIDPDGNCTDGITRTVSDETYNGGEQIK
                     DIAPSWPRAKYLNVWIVARIPGGVAGYAYYPGWVPDGTDGILIDHAYVGSIGTGSSGR
                     ARTLTHEIGHYLNLMHPWGDSNDPGLPENCEIDDEVEDTPNTIGHTSCSVYAETCGSL
                     DNVQNYMEYSYCGLMFSEGQGVRMRATLNSSICDRNNLWSESNLIATGVFDENSIAVC
                     EPVADFDQNIKVACTGREVQYYDLSYNTDTIYNWIWAFDGGTPELSDEPNPVIVYQQP
                     GHFSTGLTVSNPSGSDTKEKEGSIAVYSHSDALEAPYSFNFEDGAFPGLTGIQNNDFY
                     LVNNGSTYWMRTNSAAAEGSYSIRIRNSLNDDNISNSFYTPLMAIDTNSFPLTVYFKT
                     AYAKTDNTTNDVLRIYCSYDCGETWKIKFYLSGNGLKTTDDFQQNGTFIPTSEEWRQD
                     SFSISRPANLECNSIRLRFETISHGGNCLYIDDIQMLASVSAINVKESEQLSVYPNPF
                     TDKITIDGIEAPEVSISIFDVSGRKVGEKVYAHVYTSLEISSLMEQCTSGTYFLHISY
                     NGLTTTKIINRN"
     gene            complement(5016..>6294)
                     /locus_tag="PLK75_11675"
     CDS             complement(5016..>6294)
                     /locus_tag="PLK75_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39946.1"
                     /translation="ASGEISFARAVSCENDANNDNWDYLAFLIDGNEVQRWDGVLGWE
                     QFTYPVSSGVHTFTWKYKKDGSVNTGSDCAWIDNIIFPSIYDAPPVLVLSTDTIVKHM
                     GIDETDTDTLYVMNMGGGILNYHFEILNVIQGSGQKSIAGSTVTCDQNAFVMGSDVSW
                     AFSIYNASTDNEWIKQVYFDFPAGVTVTMVTDIIDQGGDTLITSGTLGNGVIISWTGE
                     DDNGWGIIHGNETGYLNIDASISEDFEGDLQIEYQLHGDIYGAEPHMVNGSVDLINLG
                     PPIDWLSVDPLQEQVIIGEIDTVLITFDTNGMDEGVYQCLLKIYSDADTVDIPVTLYV
                     ENVLSEQFTEESQINVFPNPSSDCFYVSVPNTAGPIQCEVFNMNGQKIWSDCFPENES
                     IVWKTNRMLPSGIYILKLTTTEDVIVKRLNLMR"
BASE COUNT         1853 a         1293 c         1294 g         1854 t
ORIGIN      
        1 ggaaaatgaa atcgtaataa atagcaagta aacgagaatg gtagccgtat gtttcagcat
       61 atcagaatcg tattgaaatg agggtctttt tatcaagact taacaatgag gctgcgtctt
      121 cttctctaat tgcaacttca agcaggtcga gagaattaaa aattaccaca aggtctcctt
      181 tttcaacctc attgtaagaa ttacatattt tatcaacatg gtacttgttg ctgttgatgt
      241 aaatatcaaa tttgcccgac ccaacgtgtt tgtcgaatgt ttcgcggttg atattggtaa
      301 tgccattgct gtaggaatcg atatatagga tgtgacctac aatcattcct tctgtaattt
      361 ttggagccaa tggtgttttg cgcttgatat catcaacttt ttcaccaaga gtagttatat
      421 ctccccctcc gagaatgtag caggcaagtt ttgagtaaat gcttaaacag ggaaaagtat
      481 aggttttggg cagactatca ggataaatat atatcaccat ttcgggttga ttgtcgaaaa
      541 caagaccaaa aatccccgtg tcggtaccta taaagtattg acctttgttt tttattatta
      601 cataaggact ctcagagttg gcttctgtat ttacacacat taggtttaca gttccttcgg
      661 gaaaagcttt gtagctgctc ctcagcacaa atgcagcacg ttgctcattg tatgcaggaa
      721 tatcgtgggt tacatcaata aaccgtacat cgggaacagc tgcaataata gctgacttca
      781 cggtacacaa ataataatcg tgattcttcc agtcagaaat tatgcttatc agtgcttgca
      841 tattttacta tttttgttga aaatagttat tgttctttgc gggtaaaaat aaaccaaatg
      901 tagttacaaa aaaacaccat atttcaaatt atgacggaaa aaagtttttt gttggaaatg
      961 gctgatccaa ggcagttatg cgggcttaaa gatgaaaacc tgtatttgct tcaacgcttt
     1021 tttgcaaaac tcaaaataat tgtacggggc gattcgctta aggtaatggg agatgaggag
     1081 gaagtggcac gttttgaatc gaaacttaaa atacttacgg ctttcatcaa tcactacaat
     1141 aagcttagtg ctgatgacgt gcaaatgttg ctgcaaaatg atgatcaggg cgaaacctta
     1201 aacttcggaa aagaaaaaga tgtactttta tatagtatca ccggtaaacc tatcaaggca
     1261 cgtacaaaaa atcagcgagc acttactgaa gctgctgttg agaacgattt gctttttgcg
     1321 gtcggtcctg cgggtaccgg aaagacctac actgccatag cccttgcggt aaaagcactg
     1381 aaagatcaca aagtgaaacg aataattctg agtcgccctg cggtggaagc aggggagacg
     1441 ctgggttttt taccgggcga tcttcgcgaa aaacttgacc catacctgca accattgtac
     1501 gatgcactga tggatatgat tccttcacgc aaactggagc aaatgctcga agacaaggtt
     1561 attcagattg ctccactcgg ttttatgagg ggacgtacgc ttaataacgc ttttgtaatt
     1621 ctcgatgaag cccagaatgc aaccttcaat caaatgaaaa tgttcctgac ccgaatgggc
     1681 gagtactcaa agtttattgt cactggcgac ctcacccaga tagacctgcc caataaaaaa
     1741 gaatcaggat tgttgaaagc cagcgaaata cttagagaga ttgatggaat aaagtttatt
     1801 tttttcgatg ctgatgatgt tatccggcat cgccttgtta catcaattat caatgcttac
     1861 gaaaaagaaa acaataacaa tcaatatgaa taaaacactc acaaaaactg attttcgttt
     1921 caaaaaccaa agtcatgtat atcatggtaa agtaagggac gtatatttta ttgatgaaga
     1981 atatattgta atggtagtca ccgacagaat ctcggcattt gatgtgatat tgcctaaagg
     2041 cattccattt aaaggccagg ttcttaatca gattgccgat aaatttctta aagccaccga
     2101 ggatatagta ccgaattgga gaatagcaac gcctgatcct atggctatgg ttgggtataa
     2161 atgtcaacca tttccggttg agatgattat tcgcggatat cttaccggca gcagttggcg
     2221 cgattacaaa aacggtgccc gcagtatttg cggtgttcca atcgctgatg gtatgaaaga
     2281 acatcaggct ttcgaaaaac cgataattac tccaacaacc aaggctgaaa tgggatatca
     2341 cgatgaaaac atttcgaaag aacagattat agtttcagga atggtttcgg aagaagacta
     2401 tcagatgctg gagagatata cttatgagtt atttaggcgg ggaaccgaaa ttgctgaaca
     2461 gcaaggatta attcttgttg acacaaagta tgagtttggc aaacgcaacg gccaaatcta
     2521 tcttatcgac gaaatccata ctcccgattc atcgcgttat ttttataagg aaagctatgc
     2581 agataatttt aaagcaggtt taccccaacg ccagttatcg aaagaatttg tccgcgaatg
     2641 gcttatttcg agtaatttta tgggtcgcca gggggagcag attccacaaa tgaccgacga
     2701 aaaagcgtcc gagatatcac aacgctatat tgagctttac gaaaacatta ccggcgatgc
     2761 atttatcccg ggtactggcg aaaacctgag tcagcgtatt gatgacaatg ttacagcctt
     2821 tctccggaat ctctgattaa ttccgattga tgattttggt agtagtcagc ccgttatagg
     2881 aaatatgcag aaaataggtt cctgatgtac attgttccat caaactgctg atttccaaac
     2941 ttgtgtaaac atgtgcatac actttttcac cgaccttacg acctgaaaca tcgaatatgc
     3001 ttattgaaac ttctggtgcc tcaatcccgt caatagtaat tttatcggtg aacggatttg
     3061 gatatacaga gagttgttcc gattccttta cattgattgc tgatactgat gcaagcattt
     3121 gaatatcatc aatataaaga caattaccac catggctgat agtttcgaac cgaagtctta
     3181 tggaattaca ttccagattt gccggtctgg aaatggaaaa actatcttgc ctccattctt
     3241 cggaagtagg aatgaaagtg ccattttgct gaaaatcgtc agtggtttta agtccgttac
     3301 cggataaata gaattttatt ttccatgttt caccgcagtc gtaggagcag tatatacgca
     3361 aaacgtcgtt agtagtgtta tcggtttttg cgtatgccgt tttaaaatac actgtcaaag
     3421 ggaaggagtt cgtatcaata gccatcaagg gggtatagaa tgaattggaa atattatcgt
     3481 cgttgagcga attacggatt cttatcgaat agcttccttc agcagctgcc gagtttgtac
     3541 gcatccaata tgtgcttcca ttgttcacaa gataaaaatc gttgttttga attcctgtca
     3601 ggccggggaa tgcgccatct tcaaaattga atgagtaagg agcttccaga gcatcactgt
     3661 ggctgtaaac ggcaatgctg ccttcttttt cttttgtgtc gctccctgat gggttcgata
     3721 cagtaagtcc ggtactgaaa tgaccgggct gctggtacac gattacaggg ttcggttcat
     3781 cggatagttc gggtgtaccg ccatcgaatg cccaaatcca gttatagatt gtatcggtat
     3841 tgtagcttaa atcgtagtat tgcacttctc ttccggtgca agcaacttta atattctgat
     3901 cgaaatcggc aaccggctca catacagcga ttgaattctc atcgaaaaca ccggtagcaa
     3961 tcaaatttga ctcagaccag aggttatttc tgtcgcaaat gctggaatta agagtggctc
     4021 tcattcttac gccctgtcct tcactgaaca tgagaccgca ataggagtac tccatatagt
     4081 tctgcacatt gtcgagcgac ccgcaggttt cggcataaac actacaactg gtatgtccga
     4141 tggtgtttgg agtatcttcg acttcatcat caatttcaca gttttcgggt aatcccgggt
     4201 cgttgctgtc gccccagggg tgcattaaat tcaggtaatg cccgatttca tgggtaaggg
     4261 tacgtgccct gcccgaggaa cctgttccta tagaaccgac ataggcatga tctataagaa
     4321 tgccatctgt tccatctggt acccagcccg gataatacgc atatccggct actccacccg
     4381 gaatccgcgc tacaatccaa acattcagat atttggctct gggccatgaa ggggcaatat
     4441 ctttaatctg ctctccacca ttataggttt catccgacac agtacgtgta atgccatctg
     4501 tgcaattacc atctggatca attttagcaa gcctgaattc aatatgagaa tcaccggcaa
     4561 tttctttaaa aggatcgatg atgtacatgg tatcggagtt cagtttcctg aaatcgagat
     4621 tcaatattct gatctgatct tcaatctgct ctttcgaaat gttttcaggg ccataattat
     4681 gcactatatg aaacaccacc ggaattacat acacctgatc tgttttttgc tcctgctcta
     4741 catattgctc aacaaaattt ctgagttcaa gaatatcgtt aattgcagca gggttagcct
     4801 ccagcgcttt acgattcatc tcaggtgtag cgcaacgcca cgatgattcc tgcgcatttg
     4861 ctggaatatt acatgccagc attcccgtaa tgataaacaa atagattatt gtttttttca
     4921 tagagtcatt tttagtgttg caaatatagg ttaaaatttt ttcaatgaat taaccgctat
     4981 ggactcaatc tgttcaaata aaaataaacg aatctttacc gcatcagatt aagtcgctta
     5041 actattacat cttcagtagt tgtaagcttc aggatataga taccggaagg cagcattctg
     5101 ttggttttcc atactatact ttcgttttcg ggaaagcaat cagaccagat tttttgccca
     5161 ttcatattga atacttcaca ttgtatcggc cccgcggtgt ttggaacaga aacataaaag
     5221 cagtccgatg atgggttcgg gaacacattt atctgcgatt cttcggtaaa ttgttccgac
     5281 agaacgtttt cgacatagag cgttaccggt atatctacag tatccgcatc ggaatatatt
     5341 tttaaaaggc attggtaaac gccttcgtcc ataccgttag tatcgaaggt tatcagtacg
     5401 gtatcaattt ctccaataat aacctgctcc tgaagcgggt caacactaag ccagtcgatt
     5461 ggaggcccga ggttgatcaa gtcaacagaa ccattaacca tgtgtggctc agccccataa
     5521 atgtcgccgt gaagctggta ttcaatctgc aaatccccct caaaatcttc tgagatgctt
     5581 gcatcaatat taaggtatcc cgtttcgttt ccatgaataa taccccagcc gttatcatct
     5641 tcaccggtcc agctgattat tacaccattg cccaaggtgc ctgaagtaat aagagtatcg
     5701 cctccctgat caataatatc ggtaaccatt gtaacagtta cacctgcagg aaaatcgaaa
     5761 taaacctgtt taatccactc gttgtcggtt gaggcattat aaattgaaaa ggcccatgaa
     5821 acatcgctcc ccataacaaa ggcgttttgg tcgcaggtta ctgtgcttcc tgcaatgctt
     5881 ttctgacccg aaccctgtat tacattcagt atttcgaaat gataattcag tattccaccg
     5941 cccatattca ttacatacag tgtgtcggtg tcggtttcgt cgattcccat atgtttaacg
     6001 attgtatcgg tgctaagaac aagcactgga ggggcatcgt aaatcgacgg gaaaatgata
     6061 ttgtcaatcc atgcacaatc ggaccctgta tttactgagc catctttttt gtatttccat
     6121 gtaaaagtat gtacacccga tgatacgggg taagtgaatt gttcccaacc gagaacgcca
     6181 tcccatcgct gcacttcgtt cccgtcaatt aaaaaagcaa gatagtccca gttgtcatta
     6241 ttggcatcgt tttcgcagga caccgctctg gcaaacgaaa tttctcctga tgcc
//
LOCUS       DAOOQP010000146         6219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_15324_length_6219_cov_4.939974, whole genome shotgun sequence.
ACCESSION   DAOOQP010000146 DAOOQP010000000
VERSION     DAOOQP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15324_length_6219_cov_4.939974"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1749
                     /gene="nhaD"
                     /locus_tag="PLK75_11680"
     CDS             385..1749
                     /gene="nhaD"
                     /locus_tag="PLK75_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOY39947.1"
                     /translation="MFILIVIVFVLGYVAIALEHPIKIDKAASALLIGVLCWTIYVLS
                     ADTILNLGFSPNWNGTGEHGETLHFVGHELNHHLIDIAQILFFLMGAMTIVEIIDQHN
                     GFRVITDKIKTTHKVKLLWIISFITFFMSAALDNLTTTIVMVALLRKLIDDRPTRWFY
                     ASMVILAANAGGAWSPIGDVTTIMLWIGGQVTTGAIITRVFLPSLASMVIPLTLLSFT
                     MKGHVTRPSNTSESAGITSNKEQLIILIIGVCALLFVPVFKTITHLPPYMGMLLGLSI
                     LWITTELMHRKKESESKKRLAVFNIIRKIDTPTILFFLGILTAVAALQSAGHLNLLAG
                     WLDDKLGNIFLIDVTIGLLSSVVDNVPLVAGAMGMYPIEAAGAVNYAANFMQDGLFWE
                     FLAYCAGTGGSILIIGSAAGVAAMGLEKIDFIWYLKKISWLALLGYLAGAGVYYLQSL
                     IIVH"
     gene            complement(1793..2497)
                     /locus_tag="PLK75_11685"
     CDS             complement(1793..2497)
                     /locus_tag="PLK75_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY39948.1"
                     /translation="MNENLHILLEDVSVAHENNIVLRNVNLRIMQGEFVYLIGRVGSG
                     KSSLLKMLYAEIPILGGNAAVLDYDIDNIQGSKVPFLRRQIGMVFQDFQLLSDRNVEE
                     NLKFVLKATGWKNETEMNNRIGELLISVGMLDKLKKMPHELSGGEQQRVAIARATLNN
                     PPMIFADEPTGNLDPESADNIMNILLDLNRSGKTIVMVTHNYAVLKKYPARTVLCENG
                     EIRELPNDEAIDMSDL"
     gene            2711..5854
                     /locus_tag="PLK75_11690"
     CDS             2711..5854
                     /locus_tag="PLK75_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY39949.1"
                     /translation="MNIKVFLLMILLLAGLFAFGQKTMLYNEPDDRYAGAVQLYEAGH
                     YGAAQKIFEEFASSDKPENSVYKDDAKYYASMCALKLFNKDAEYLISVFIREHPESYN
                     VNDANFQMGNYQFLKKKYPSALKWYEEVDRLSLTAEENGEYFFKTGYCHFARKDYDKA
                     ANAFYEIKDGSTFYAPIAEYFYSHIKYMNQQYQTALLGFKKLENNELFADIIPYYITQ
                     IYYMQSKYEEIIAYAPDVLKKSNGERMFEVARIIGEAYYRTGKYNDALEYLEMSHPEN
                     ASLSDMDAYQLAFAYYKTQNYAKAIPLFESVVNVDDTLSQNAAYHLADCYIKTNRKKD
                     AKLAFGAASKLSYNQTIREDALFNFAKISFDLDMSPFNEALNAFTTYLNEFPQSPRVD
                     EVYDYMLQAFATTKNYQDALGIMDKMKNRPPKIDQEYQRIAFLRGQECFIDSKFEKAI
                     GFFDKSLQFKQYDPVVKAQSYYWKAEAYYRMERYNESIEIYKTFVESSGAVNLDEFAT
                     AHYNIGYAYFVQKDYTNAGNWFRKFEKQYEGGNEKLYNDALNRIGDCYYISKQFEPAT
                     EYYKKSAQLGKYDSDYAMYQMALGYGGQKQHQEKVWSLTRLVREYPNSNYSGFANFEI
                     ARTYSTMLNKPDSAIFYYNKVISDYSGTPMIKPALSDLATLYFDLKRYNEALESYKQL
                     IALYPNSDEARTAKDMIRTIYIAMNDTDGYVEYANTPGSNLVVTVTEQDSLTFLAAQK
                     LYVNQEYERALEAIEKYISKFPQGAYLTEAHYYRAELLYYFKRSDEALPSYEIVATAP
                     IGMYTEESVLKAASINYLNKKYDKALPYYVKLDNISKSKNNILTARLGQLRCNYILGK
                     WDDVITSAKNLTETENAGEDLIKEARYDEAKARYNLQQWVQAYNLFSAISTEVNSYEG
                     AEARFRMAEIQLKIGRDTLAENMIYEFAQMSSPHRYWLAKSFILLADIFLSRNDVFQA
                     KHTLQSVLDNYTDDTDGIKDEAFTKLAEINEKEKADEQSNDFLKMEIDLGGSDDGYEK
                     LIEPEKTENEKPENNNQ"
     gene            5938..>6219
                     /locus_tag="PLK75_11695"
     CDS             5938..>6219
                     /locus_tag="PLK75_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39950.1"
                     /translation="MKTVLLIALFAIIDCAFAQSQDTARPLNSGDITIYNEYTPSIRT
                     ANRLQYMPVIIDTVKVQPRFSYSINSVQYPTLFAPAPLQSAELKGEPLKP"
BASE COUNT         1952 a         1219 c         1253 g         1795 t
ORIGIN      
        1 tatgtggtag gcttttttgc attattcctg attctgggtt gggagcctgc atcaaacaaa
       61 gagcatgcag agcctgcaat cgaatgcaaa gccgctgatt gccctgtaag agaaaaagcg
      121 ctggaattga aaaatcatga gcttgaagca acgaaaaaat gattcttcgg gacgtgcttt
      181 gaacatcagc tttaatttat gttaaacaac atataattaa aaccctttaa aaactgcaaa
      241 cattactatt acaaaagttt gcagtttttt tattttgact ttcaaaccaa attaactccc
      301 tatactaccc aatgaggtaa atcactttga tatttagtgt caaagtttat ttttgcagtt
      361 taattaaagc attgacatat gagtatattt atattaattg taattgtttt tgtactgggt
      421 tatgttgcca ttgcccttga acaccctatt aaaatcgaca aagctgcaag tgcgctttta
      481 atcggagtat tgtgctggac tatatacgta ttatcggctg atacgatttt aaaccttggg
      541 ttcagtccaa actggaatgg gactggcgaa cacggagaaa ccttgcattt cgtcggacac
      601 gaattaaatc atcatttgat tgatatcgcc caaatattgt ttttccttat gggagccatg
      661 actattgttg aaataattga tcaacacaac ggctttcgag ttataactga taaaatcaaa
      721 actactcata aagtaaaact actttggata attagcttta ttaccttctt catgtcggca
      781 gccctcgaca atcttaccac tactatagta atggttgctt tgcttcgaaa gttgattgac
      841 gacagaccaa cccgctggtt ctatgcaagt atggttatac tggcagccaa tgcaggaggt
      901 gcatggtcac caatcggaga tgttaccacc attatgcttt ggattggagg gcaggtaact
      961 acaggggcca ttatcacccg ggtatttctg cccagtctgg cttcgatggt tattccgctg
     1021 acattattat cttttactat gaaaggacat gttacacgac cttcaaacac aagtgaatca
     1081 gcaggtataa catcgaacaa agaacaactt atcatactta taatcggggt atgcgctttg
     1141 ctttttgttc ccgtttttaa aacaattact catttacctc cctatatggg aatgcttttg
     1201 gggttaagca tactgtggat tactactgaa ttaatgcacc gcaagaaaga aagcgaaagt
     1261 aaaaagcgat tggctgtatt taatatcatc agaaaaatcg acacgccaac aattttgttc
     1321 ttccttggta ttttaacagc agtggctgca ttgcagagcg ctggacattt aaacctgttg
     1381 gcgggatggc tcgatgataa acttggaaat attttcttaa ttgacgtaac cattggatta
     1441 ttatcatcgg ttgtcgacaa tgttccattg gtagccggag ctatgggaat gtacccgatt
     1501 gaagctgccg gtgctgttaa ttatgctgct aacttcatgc aggatggttt attctgggag
     1561 tttttagctt actgtgccgg tacaggaggc agcattctta tcattggttc ggccgctggt
     1621 gttgctgcca tgggtttaga gaagattgac ttcatatggt atctgaaaaa aatatcgtgg
     1681 cttgctttac ttggatacct tgctggagca ggcgtgtatt atctgcaatc gttgataatt
     1741 gtgcactaga ggatgtcgtt aatttttacg gattaaccgt tatgggaacc tatcagaggt
     1801 cgctcatatc gatggcttca tcgtttggta attcacgaat ttcgccattt tcacacaaca
     1861 ctgtgcgtgc aggatacttt ttaagaacag cataattgtg ggtaaccatc acaatggttt
     1921 taccggagcg gttaaggtca agcaatatat tcataatatt gtcagccgat tctggatcga
     1981 ggtttccggt gggttcatcg gcgaatatca ttggcgggtt gttgagtgtt gctctcgcaa
     2041 tggcaacgcg ttgctgttcg ccacccgaca gttcatgagg cattttcttc agtttatcca
     2101 acatcccaac gcttattagc aattcgccaa tcctgttatt catttctgtt tcatttttcc
     2161 accctgttgc ttttagcaca aacttcaggt tttcctcaac attccgatca cttagtaatt
     2221 gaaaatcctg aaaaaccatg ccgatttgtc tgcgcagaaa cgggaccttc gaaccctgaa
     2281 tattgtcgat atcatagtcg agtacagcag cattgccccc caatatagga atctcagcat
     2341 aaagcatttt cagaaggctc gattttccac tacccaccct tccgattaaa taaacaaatt
     2401 ctccctgcat tatgcgaagg tttacattcc gaagaacaat attgttctcg tgagctaccg
     2461 aaacatcttc gagaagtatg tgtaagtttt cgttcattgg gtacaggcaa ttttgtaaat
     2521 aaagcgttcg ttattgtaat aaatacaaat ctaacgaaaa ttaaaaggag caagagcctc
     2581 agatgcatta gaaataaaca aaaacctgtt aattaaaaca tgaatatgtt ctgaagcaac
     2641 gaaactttaa atagtacttt tattttctaa ttgtctataa tttattaaaa tataaataac
     2701 gtgtaatatt atgaatatca aggtatttct tttaatgata ctccttctgg caggcttatt
     2761 tgcttttggg caaaaaacca tgctttacaa tgaacctgac gaccgctatg caggagcagt
     2821 tcaattgtat gaagccggtc attacggtgc tgctcagaaa atattcgaag aatttgcaag
     2881 ctcagataaa ccggaaaatt cggtgtataa agacgatgca aaatattatg cttcgatgtg
     2941 tgctcttaaa ctatttaata aagatgccga atatttaatc agtgttttca tacgtgagca
     3001 ccctgagagc tataatgtga acgatgcaaa ttttcagatg gggaattacc agtttctgaa
     3061 aaaaaaatat ccctcagctt taaaatggta cgaagaagtt gatcggttaa gtcttaccgc
     3121 agaagaaaat ggggaatatt tcttcaaaac agggtactgc catttcgcaa gaaaagatta
     3181 cgacaaagca gcaaatgcat tttatgaaat taaagacgga tctacttttt atgcacctat
     3241 tgcagaatat ttttactcgc atatcaaata catgaatcag cagtatcaga ctgcgttgct
     3301 tgggtttaaa aaactggaga acaacgaact ctttgccgat ataattcctt attacattac
     3361 tcagatttat tacatgcaaa gcaaatatga ggaaattatt gcctatgctc ccgatgtact
     3421 gaaaaaatcg aacggtgaaa ggatgttcga ggtagcccgt attattggtg aagcatatta
     3481 ccggaccggc aaatacaacg atgctttgga atatctcgaa atgagtcatc ccgaaaatgc
     3541 aagcctatcg gatatggacg cgtaccagct ggcatttgcg tattataaaa cccaaaatta
     3601 tgcaaaagct ataccgcttt tcgaatcggt cgtaaatgtt gatgacactc tgtcgcaaaa
     3661 tgctgcgtat catctggccg actgttatat aaaaacaaat cgcaagaaag atgcaaagct
     3721 tgcttttgga gctgcttcca aattaagcta taatcagact atccgtgaag atgcgctttt
     3781 caattttgca aaaatatcgt tcgaccttga tatgtcgccc ttcaacgaag cgctgaatgc
     3841 atttaccacc tatctgaatg agtttcctca atcgcccagg gttgatgagg tgtacgatta
     3901 tatgcttcag gcatttgcaa caaccaaaaa ctatcaggac gcattaggta taatggataa
     3961 aatgaaaaac cgccctccaa aaattgatca ggaataccaa cgcattgcat tcttacgtgg
     4021 tcaggagtgc ttcattgatt caaaatttga aaaagcaatc ggatttttcg acaaatcgct
     4081 gcaattcaag caatacgatc ctgtggttaa agctcagtca tactactgga aagccgaagc
     4141 ttattacaga atggagagat ataacgagtc tattgaaatt tataaaacct ttgttgaaag
     4201 ctccggagct gtaaaccttg atgaatttgc cacagcccat tacaatatag gttatgccta
     4261 cttcgtgcag aaagattata caaatgcagg aaactggttt cgaaaattcg aaaaacaata
     4321 tgaaggtggt aacgaaaagc tgtataacga tgcattgaac agaattggcg attgctatta
     4381 catttcgaaa cagtttgaac cggccacaga atattacaaa aagtcggctc aattgggtaa
     4441 gtatgattcg gactatgcca tgtatcagat ggctttaggg tatggaggtc agaaacaaca
     4501 ccaggaaaaa gtatggtcgc ttacccggct tgtacgggaa tatccgaatt cgaattattc
     4561 aggctttgcc aattttgaga ttgcaagaac ctacagtacc atgctgaaca aacctgattc
     4621 tgccattttc tattataata aagtgattag tgattattcg gggacgccta tgattaaacc
     4681 cgcattatcg gaccttgcca cattgtattt cgatctgaaa aggtacaacg aagctctcga
     4741 aagctacaag caactgatag cattgtatcc aaattctgat gaagcccgca cggcaaaaga
     4801 catgattagg accatatata ttgcaatgaa cgacaccgac ggatatgtgg aatacgcaaa
     4861 caccccaggt agtaatctgg tagttacagt aaccgaacag gactcgctta catttctcgc
     4921 agcacagaag ctgtatgtta atcaggagta cgaacgggct ctcgaagcca tcgaaaagta
     4981 tatcagtaaa ttcccgcaag gagcgtacct taccgaagcc cattactaca gggcagaatt
     5041 attgtattat tttaagcgtt ccgacgaagc acttccctcc tacgaaattg ttgcaactgc
     5101 tcccattggc atgtacaccg aagaaagtgt actgaaagcc gcatcaatca attatctgaa
     5161 taagaaatat gataaggccc tgccctatta cgttaagctt gataatatat cgaaatcgaa
     5221 aaataatatt cttactgcaa gacttggaca actccgttgc aactacatac ttgggaagtg
     5281 ggacgatgtg attacttcgg ctaagaacct taccgaaacc gaaaatgcag gtgaggacct
     5341 cataaaggaa gcacgctatg atgaagctaa ggcaaggtat aatttgcaac aatgggtcca
     5401 ggcatacaat ctgttttcag caatcagcac cgaagtaaat tcatacgaag gggctgaggc
     5461 caggttcaga atggctgaga tacagcttaa aatcggtcgc gatacactgg ccgaaaacat
     5521 gatttacgaa tttgctcaaa tgagttctcc acatcgttat tggctggcaa aaagttttat
     5581 tcttctggct gatattttct taagccgtaa tgatgtattt caggctaaac acactctgca
     5641 atcggttctg gacaattata ccgatgatac agacggcatt aaagatgaag cgtttaccaa
     5701 gttagcggaa atcaatgaaa aagaaaaagc cgatgaacaa tccaacgact ttctgaaaat
     5761 ggaaattgat ctcggtggca gtgacgacgg ctacgagaag ctgattgaac cggaaaaaac
     5821 ggagaatgaa aaacccgaaa acaataatca atgacaatac atactttaga tactatgaaa
     5881 aaagtttgca acataattcg gctaagccgc ttcgcaagaa accggaggtc tgctataatg
     5941 aagactgtgc ttttaattgc attgtttgct attattgatt gtgctttcgc acaaagccag
     6001 gacaccgccc gcccattgaa tagtggcgat atcactattt ataacgagta cacacctagc
     6061 atacgcactg ccaacagact acaatacatg ccggtaataa ttgatactgt aaaggttcag
     6121 ccgcgtttca gctatagcat caattcggtg cagtatccta ccctgtttgc ccctgcacct
     6181 ttgcaatcgg cagagttgaa aggagaaccc ttgaaacct
//
LOCUS       DAOOQP010000147         6113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_15661_length_6113_cov_5.123473, whole genome shotgun sequence.
ACCESSION   DAOOQP010000147 DAOOQP010000000
VERSION     DAOOQP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15661_length_6113_cov_5.123473"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1785
                     /locus_tag="PLK75_11700"
     CDS             397..1785
                     /locus_tag="PLK75_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOY39951.1"
                     /translation="MFHSDIYLNRRQSLAKQVGKGIVLLLGNNESPMNYPDNGYHFRQ
                     DSTFLYFFGLDLPGMAAVIDTESNSTTIFANDVDIDDIIWMGPQPSVKELAMKCGVTE
                     TQTFGSLNEYLKNAIQKKSIIHYLPPYRHDNKILLHQMLNIPFESLKQSASVELIKAV
                     VSLRNHKEPCEIEEIDRACNIGYKMHVAAMKMAKAGVVEREIAGAMEGISLSYGSVPS
                     FPIILTKNGQTLHNHYHGNVLKNGDLLLADAGAQTPMMYASDNTRTMPVGGKFSQKQK
                     EIYQAVLNANNHATSIMKPGITNFDVHMAACRVLASDLKDIGLMKGDISEAVALGAHA
                     LFMPHGLGHMMGLDVHDMEDLGQIYVGYDETVRPSNIFGTAFLRLGRKLEEGFVLTNE
                     PGCYFIPELIDLWHSEHKFEDFINYHKVNQYRDFGGIRLEDDILITKTGSRILGEKRI
                     PISITEVEQTMA"
     gene            complement(1802..4447)
                     /locus_tag="PLK75_11705"
     CDS             complement(1802..4447)
                     /locus_tag="PLK75_11705"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOY39952.1"
                     /translation="MKKYILFFALFLVYISSVYAQTNNETGKYEGKMRYSSSHSNSIP
                     LPAINKSTVFFLEDFEAGVIPDEWTTISGDCNWFVTENGSSTYFSIPDHTTYVCVSDD
                     GCNANMSNVWLITPTINLSGVLQPELSFDVYGYSDLLTIKISNDNGNTFADLQQLSDC
                     YEWTHITIPLSDYAGQTDIKIAFHYNDQGYWGYGYCLDNISIRVQSINDLSVVFANPV
                     YVSSGDSPYPSVIVKNSGVNTQNNFSVDIVITDGTGGEAYSSSVLSTDVFLASSEEVE
                     ITMPDPWVTPELGQYNIKAFVSTPIDEDPTNDTLFATCNVLIISNSYTVDALDNNFVF
                     MDVPVGNETVFGDFDCSNFPMEMEFAADGNYYLIRSASGDGTVPAKLSTYDYITETEN
                     VIGDLTGIAGIPTAIAYNWATDTMFIVFINEENQPVLGTVDLSTAECDIIGTGTGLII
                     AMDFDDNDTLYGISIDDDMLYQIDLISGYTDAVGYLYTDINYGQDVSFNRQTGVMYGL
                     LFGNSPGLYSINLQTGEATMLAAKTHQYACFEIEEYPPCQQPSEITVVDLTTESALLQ
                     WENPGSAFGWNAEWGEEGFAPGEGTLESEILDNLFTLSGLSSNTTYSFYLQSICEVGL
                     SSWVGPFSFSTLPFCETQNSLVEGFEGGEIPLCWDNIQNGAGNNTWSVTSQSPYSGAY
                     AAFAMFEASGNENQQWLITNSILIPQNQKLGFFVKDHFSTDNASQLKLFISNDQQTKP
                     MSYIELLTVNESDVSNTEYTHFEADIADFSGQQVCFAFVMIDNNGDSWYIDDINMETN
                     VNSNLESPENIRIYPNPCNNEVIIMNARGASIEVINLTGQTILSFDKEYNNTHYNLES
                     LSAGNYFVRITKNNTTVVKKLSIIR"
     gene            complement(4535..>6113)
                     /locus_tag="PLK75_11710"
     CDS             complement(4535..>6113)
                     /locus_tag="PLK75_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39953.1"
                     /translation="IVLEATGMDANLTWDAPTAGLHGGYFSGTGLTYDVVRYPGGILV
                     SDDQTELTFAETLTDANNYFYEVTPSNAAGEGATGTSNTLVIGDVVFFDFETGLPTNG
                     QLVNGDCGWLFGPNGSSTYFPIPAHGNYAYANDDQCNADMSDVWLILPPVDFTGIVNP
                     YIAFTNVRYYDIFTIKASTDGASWTDVTTLSADNINTWQDENVDLTAYANAPNVFIAF
                     HYNDNGVWAYGWAVDDVMLPGSLVEITCPAPVDIAAITSPGTATISWTETGSATAWNL
                     QYGEAGFTFGEGTTATATTNPQAELTELAAGVYDVYVQADCGGSQSFWAGPFSFVVPN
                     CENSDMCDYVFTLIDDYGDGWNNASVIIFEGDLPVTEITLPDGETNTVNVSLCTGMNI
                     SLYFVSGNYDIEVGLTVADPEGTTIATYAVGYFDGMETGTMFGSFTSDCSVSVENIIS
                     DNIILYPNPSNGLVTITNAEGASIEVINVMGQVIASFDANSSLTTFDATNLSNGNYVV
                     RIVKDNNTTVKTLNIIK"
BASE COUNT         1835 a         1258 c         1267 g         1753 t
ORIGIN      
        1 cctcccgtca ttgcgagggt tgaaaacccg aagcaatctg tatttcgacg ggtggtgttg
       61 ttgctccttc gtggatgtgg attgcttcac tgcgttcgca atgacgttta ggagctcgtc
      121 attgcgaatg tgaggtacga acatgaagca atctgtattt cgacgagctt agcacaagaa
      181 cttctttgac ggaattaata cagacatttc aaaaggttgt gttgtaacgt cttcgtggat
      241 gtggattgct tcactgcgtt cgcaatgacg gttgagagcc cgtcattgcc tttagattag
      301 caccgtaaaa attagttgta attttatggc ataaactgta ttaatcataa actgtttttt
      361 tgtagtttta ggctgattta ttaaattaat taaaacatgt ttcattcgga tatttattta
      421 aacagaagac aaagccttgc caaacaggtt ggtaaaggta ttgtactatt attaggcaac
      481 aacgagtccc cgatgaatta ccccgataac ggatatcatt tcaggcagga ctctacattt
      541 ttgtattttt tcgggctcga tttgccggga atggctgctg tgattgatac cgaaagtaac
      601 tcaacaacca tttttgcaaa tgatgtcgat atcgacgata tcatctggat gggtccccaa
      661 cccagtgtta aggagcttgc aatgaaatgc ggtgtaacag aaacacaaac ctttggcagt
      721 ttgaatgaat acctgaaaaa cgcgatacaa aaaaagagta taattcatta cctgccgccg
      781 tatcgccacg ataataaaat attgctgcat cagatgctca atattccatt cgaaagcctc
      841 aaacagtcag cttcggttga gctgatcaaa gcagttgttt cgctcaggaa ccacaaagag
      901 ccctgcgaaa ttgaagaaat cgacagggct tgtaacattg ggtataaaat gcatgttgct
      961 gcaatgaaaa tggcaaaagc cggtgtggtt gagcgcgaaa ttgccggtgc gatggaaggg
     1021 atttcattat catacgggtc ggtgccttcg tttccgataa tacttactaa aaacggacaa
     1081 acgcttcaca atcattatca cgggaatgtt ctcaaaaacg gagatctgtt gcttgccgat
     1141 gccggtgcac aaacgcccat gatgtatgcg agcgataaca cccgcacgat gccggtagga
     1201 gggaagttct cgcagaaaca aaaagaaatt tatcaggctg ttctcaatgc aaacaaccat
     1261 gcaacatcca tcatgaagcc gggtattacc aattttgatg ttcatatggc tgcatgtagg
     1321 gtactggcgt ccgacctgaa agatatcgga ctgatgaaag gtgatataag cgaagccgta
     1381 gcgctgggcg cacatgcatt gttcatgcct catggcctgg ggcacatgat ggggctcgat
     1441 gttcacgata tggaagatct cgggcagatt tatgtaggtt acgatgaaac tgtccgtccc
     1501 agcaatattt tcggaacggc atttttacgg ttaggtcgta agcttgaaga aggctttgtt
     1561 cttacaaatg aacccggatg ctattttata ccggagctga ttgatttgtg gcatagcgaa
     1621 cataaattcg aagacttcat taattaccac aaagtgaatc agtaccgcga ttttggtggc
     1681 atccgcctcg aagatgatat actcatcaca aaaacaggct caaggatatt gggtgaaaaa
     1741 cgtatcccca taagtattac cgaagttgaa caaacaatgg cctagcgaaa ggttatccgg
     1801 tttatcgaat gatgctgagt ttctttacaa ccgttgtatt gtttttggta atccgaacaa
     1861 aataatttcc ggcactaagc gactcaagat tataatgtgt attgttatat tctttgtcaa
     1921 atgataatat cgtttgccct gtaaggttta tcacttcgat gcttgcgcca cgagcattca
     1981 taattatcac ttcattgtta catgggtttg ggtaaatcct gatgttttcc ggcgactcaa
     2041 ggtttgaatt tacattggtt tccatgttta tatcatctat ataccagcta tctccgttat
     2101 tatcgatcat cacaaaggca aagcacactt gctgccccga aaaatcggca atatcggctt
     2161 caaaatgagt gtattcagtg ttgctgacat ccgactcatt cacggtaaga agttcgatat
     2221 aggacatggg tttagtttgc tggtcgttcg aaataaatag tttcaactgc gaagcgttgt
     2281 cggtcgaaaa atgatctttt acgaaaaagc ccaatttctg attttgaggg attaaaatgc
     2341 tattggtaat cagccattgc tggttttcgt ttccgctggc ttcgaacatg gcaaatgcag
     2401 cataggctcc tgaatatggt gattgagatg taacagacca tgtgttattc ccggcaccgt
     2461 tttgaatgtt atcccagcaa agtgggattt ccccaccttc gaaaccttca acaagagagt
     2521 tctgcgtttc acaaaaaggc agggtactaa aagaaaaggg tcctacccag ctgcttaagc
     2581 ccacttcaca aattgattgc aggtaaaaac tatacgtagt gttgcttgat aatccggaca
     2641 aggtaaataa attatccaga atctcagatt caagcgttcc ttcacccgga gcgaaccctt
     2701 cttcgcccca ctctgcattc cacccaaacg cagatcctgg gttttcccac tgcaaaagcg
     2761 cactttcagt tgttaaatca accactgtta tttcgctggg ttgctgacag ggagggtatt
     2821 cttcaatttc gaaacatgca tactgatgtg tttttgcagc cagcattgta gcttcaccgg
     2881 tttgcagatt tatagagtat agacccggac tatttccgaa aagcaatccg tacatcacac
     2941 cggtttgccg gttgaatgaa acatcctgac cgtaattaat gtcggtgtac aggtagccta
     3001 ctgcatcagt gtatcccgaa ataagatcta tctggtacag catgtcgtca tcaatcgaaa
     3061 ttccatacaa agtatcgtta tcatcaaagt ccatggcaat gataagccct gtccctgttc
     3121 cgataatatc acattctgcc gtagagaggt caactgttcc caaaacgggt tgattttctt
     3181 catttataaa aacaataaac atagtatctg ttgcccagtt atatgcaatt gcagttggaa
     3241 tacctgcaat acctgttaaa tcaccaatta cattctcggt ttcagtaatg taatcatagg
     3301 ttgaaagctt tgcaggtaca gttccgtctc cgcttgctga acgaatcaga tagtaattgc
     3361 catcggcagc aaactccatt tccattggga aattgctgca atcaaagtcg ccgaaaaccg
     3421 tttcgttacc taccggaaca tccatgaaaa caaagttgtt atcgagtgcg tcaacggtat
     3481 acgaatttga aataatcaat acgttgcagg tagcaaacag agtgtcgttg gtgggatctt
     3541 catcaatagg tgtgcttaca aaagctttta tattatattg ccccaattcg ggagtgaccc
     3601 atgggtcggg catggtaatt tcaacttctt cgcttgaagc cagaaacaca tcagtagaaa
     3661 gtaccgacga tgaatatgct tctccgcctg taccatcggt aataactata tcaacactaa
     3721 aattattctg tgtgtttacg cctgagttct taacaataac cgagggatag ggagaatcgc
     3781 cgctgctaac ataaacagga tttgcaaata caacactgag gtcgttaatg ctttgaaccc
     3841 tgatactgat attgtcaaga caatatccat agccccaata gccctgatcg ttatagtgaa
     3901 atgcaatttt tatatcagtc tgcccggcat aatcgctcag aggaattgta atatgagtcc
     3961 attcataaca atctgaaagc tgttgaaggt cagcaaaggt gttgccattg tcattcgata
     4021 tttttattgt tagcaaatcg ctatacccat acacatcaaa cgaaagctcc ggttgcaata
     4081 cgcctgaaag attgattgtt ggtgtgataa gccaaacatt cgacatgttt gcattgcacc
     4141 catcatcact cacacataca taggttgtgt gatcagggat agaaaaatat gtactgctgc
     4201 cgttttctgt tacaaaccag ttgcaatcgc cggaaatagt ggtccattca tctggaatta
     4261 caccagcctc aaagtcctca agaaaaaaca cagttgattt attaattgca ggtaatggta
     4321 ttgaatttga atgactgctt gaatagcgca tttttccttc atatttaccg gtttcgttat
     4381 ttgtttgtgc ataaaccgaa gaaatgtaca ctaaaaacaa tgcgaaaaac agaatgtatt
     4441 ttttcatggt acagagttta ttgttattgc gattatacta caaatataaa aaaaaagggt
     4501 ggtaagcacc accctttaaa taattagatt aagattattt aataatattc agagttttta
     4561 ctgtagtatt attgtctttt acaattctta caacataatt accgttgctc agatttgttg
     4621 catcgaaagt tgtgagtgag ctgttagcat caaaactggc aataacctga cccataacat
     4681 tgattacctc gatggaagca ccttcggcat tggtaatggt aaccaaaccg ttcgaaggat
     4741 tagggtataa gattatgttg tcagaaatga tattttcaac gcttacgcta caatcgcttg
     4801 tgaaggatcc gaacatagta cctgtttcca ttccgtcgaa atatccgaca gcataagtgg
     4861 caatagtggt accttcagga tctgcaactg taaggccaac ttcaatatca taattcccgg
     4921 aaacaaaata caatgaaata ttcattcctg tacataaaga aacattaaca gtattagttt
     4981 cgccatctgg aagtgtaatt tctgtaacag gaaggtctcc ttcaaaaatt ataaccgaag
     5041 cattattcca tccatcacca taatcatcaa ttaaggtaaa aacataatcg cacatatccg
     5101 aattctcgca gttgggaaca acgaatgaaa atggaccagc ccagaagctt tgactgccac
     5161 cgcaatcagc ttgtacatat acgtcgtaca caccagcagc gagttctgtt aattctgctt
     5221 gaggattagt agtcgctgtt gcagttgttc cttctccaaa agtaaaacct gcttcaccat
     5281 attgtaaatt ccatgctgtg gcagagccag tttcagtcca tgaaatagtt gctgttccgg
     5341 gagaagttat ggcagcgata tcaacgggag cggggcaggt aatttcgact agagagccgg
     5401 gaagcattac atcatcaaca gcccaaccat aagcccaaac gccgttgtcg ttataatgaa
     5461 acgctataaa aacattagga gcattggcat aagctgttaa gtcaacattt tcatcctgcc
     5521 acgtattaat gttatctgct gaaagcgttg taacatcagt ccatgaggca ccatctgtcg
     5581 aagcctttat ggtaaatatg tcatagtagc gcacattggt aaaagcaatg tatgggttga
     5641 caattccggt gaaatcaacc ggaggaagta ttaaccatac atcactcata tcggcattgc
     5701 actgatcatc gttagcatag gcataattac catgagcagg aattgggaaa taagtactac
     5761 ttccgtttgg tccgaaaagc cagccacaat caccgtttac taattgtccg tttgtgggca
     5821 atccggtttc aaaatcaaag aaaactacat ctccaattac tagagtgttg gaggttccgg
     5881 ttgctccttc gcctgcagcg ttgctggggg taacttcata gaaataatta tttgcatcag
     5941 taagtgtctc ggcgaaagtt aattcagtct gatcatccga aaccaaaata ccgccaggat
     6001 aacgaactac atcataggtt aagccggttc cagaaaagta tccgccatgc aaacctgcag
     6061 taggtgcatc ccaggtaaga ttagcatcca ttcctgtagc ttcaagaact atg
//
LOCUS       DAOOQP010000148         5977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_16131_length_5977_cov_4.795846, whole genome shotgun sequence.
ACCESSION   DAOOQP010000148 DAOOQP010000000
VERSION     DAOOQP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16131_length_5977_cov_4.795846"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLK75_11715"
     CDS             complement(<1..1028)
                     /locus_tag="PLK75_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39954.1"
                     /translation="MNIKRLIFSALLLIPLLTRPCTEGLIIGEFTHDSRPIHWKLRMW
                     TGTNRLIFWDNDTNNDGIPDTDHDGNGKPDTYAFLGIRSNTNEHPNFLNPMMGLNEAG
                     FSIGITVVGNVVKGNTNPVMIYPLCHISSFSEFFNFLKSCPGVYDERNETWTIKNNYG
                     VMDAGGKAAEFEYHRSDKTTCKVRVYSATDPVRKNVKYQYGKNTNMFGIVCRANEFWS
                     DAPTGEYVEGATAFASAGRFSNAMNNMAALKPGGVSILKLLQGDNPQAGNFAHMVLRR
                     SVVSEPDSNCSTMIVQGVLEGENPLLAVFWAVMGHSDFSVALPVWILGVKNDTDKLPP
                     QCTASANE"
     gene            complement(1041..1739)
                     /locus_tag="PLK75_11720"
     CDS             complement(1041..1739)
                     /locus_tag="PLK75_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOY39955.1"
                     /translation="MKTLYLLLLFVITSIGLTAQNEPIKKYYQTGELLSVGAYNENDM
                     ETGIWEFYHKNGQLMSSGNFENGVVSGEWKFYHENGHLESIGMVENNVKTGEWKLYHD
                     NGQLQYCGKFTKGIKTNEWKSYYQNGIPESVGYYANDAKNGHWQYFDTDGKMMSEGTF
                     VNDLESGIWKVYYPNGNIEMTGNLVDGVQEGEWKFYYENGKIQEQGRFENGERKGEWV
                     EYDSNGNELERMTY"
     gene            complement(1765..>5977)
                     /locus_tag="PLK75_11725"
     CDS             complement(1765..>5977)
                     /locus_tag="PLK75_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOY39956.1"
                     /translation="LKYYKTDTVFLNPDIIYDKYNRIIIGIFDSLEWCYKQENDTLNL
                     LVNSKNRAGYYTENNQYDEAAAIYLSLADHYNKAGDSGAEASIYRSLYEVECYAGDTM
                     KANTYLLAAAGLYYEAEDYNRAIKSYITLAQTEKASDPEKAFKFLLRAEMAADKMPED
                     LYGVSYTYINIAKVCKDLGKNKYAVLLSKKTGINIVDYHASFYSRSFGDLLDDENKFV
                     DGAKDQLFREIFPEAVSSSDSASFLMLLALAIIDSDINENAMPLFNDAIAMYRRGNDL
                     IGEKVAYDVMNYLYMETNALNSYTNSYNADIKTWLPRYEKFVSGIYDESKELYNAKNY
                     EQACDTLLYLSACARIAGDEPTYVQLLINIADILYETNKNEKAKILLNKTLSIIENSD
                     EQDFLEEEYADVCLALAQIYYDEDRYYEAAEWADEALNYAYYTITKSFANLMLSLCYT
                     ETEPEEPDLAFSYAQTALTGAKKSKEPVAIHDACTQLIRLYMFSIYQMPDYSAAGKYL
                     HKCEKLKLPATENYRLMEESYLRAQYLFRTGKPEAALREVEDLESNLTSYPNFYYGDV
                     FSLHGTILKLIGHLPEALTYFEKAIQFEKQHNGKELSSVSYNHIGWIYERYGEPDKAL
                     EYHEKALALQREKNEYDDMAYSLLNIAGIYKDQGDAEMALRRMHEALEIAESRDLFYP
                     LANAYQSLGNYALSENNDADAALDYLENAYYYASEMDDRVSLSWTYSYMGKAYLNKGD
                     YENAIIYYNKSIKTSSKTGVFYGNYDAKLGLAQSYLNMQDVSNAKKYANEAYTEAAKK
                     NEPTALYDCLKMLNKIAVYEKNWNAADSYIPQILQHQNKLMMSNFATMAEEEQANYFN
                     TLSGDFMNFYSYCLFRNLQNPGLSAMAYDNIMKHKGILLKSSTSMRNAVYNSGDRQLI
                     ENYKNWILTKNMIADKYAAGEEASQLEQQANEIEKELVKSSQLFSDFNKIQDYSWKDV
                     QKNLKHDEVAIEFIHFNWHNFDAAIVDNATDSVIYCALIVDKNCQQPLLLPMFSEDEL
                     QRILLALGSETNFLIIDALYGSNRGVTFVDSAYRNVDYSEQLYSLLWQPLEKYLQGIK
                     TVYYAPDGMLHSIAFSALPYNDSLFLCDKYTLHYCASTASLAYETRSSFRKPAGNEIW
                     AYLYGGINFDMQFDESQQSNIDSTVVNRNKNRSVYIPSNSRGESWTYLPGTLEEIEQI
                     GKIFDENTFPYQIITADNGTEDIFKAQSSSNPVQVIHIATHGFYFPQTAYGTMEQGLR
                     FSVSKNPMVRSGLIMAGGNTTWSGFPPYSGKGDGILTAFEVSCLDLNKTSLVVLSACE
                     TGLGDVKGSEGVYGLQRAFKMAGADYLIMSLWKVADTETAEFMTLLYQNLTQTLDVNQ
                     AFVNTQQEMRKKYPPYYWAAFVLVE"
BASE COUNT         1576 a         1310 c         1191 g         1900 t
ORIGIN      
        1 ttttcgtttg cggaagcagt acattgtgga ggcaatttat cagtatcgtt ttttactccg
       61 agaatccata ccggcaaggc taccgagaaa tcgctatgcc ccattaccgc ccaaaacacg
      121 gccagcaatg gattttcgcc ctcaagcaca ccctgcacaa tcattgttga acagtttgag
      181 tcgggttccg aaacaactga acgccgtagc accatgtggg caaagttacc tgcctgcggg
      241 ttatcaccct gaagcagctt gagtatagaa acaccccccg gcttcagggc ggccatattg
      301 ttcattgcat tcgaaaaacg tccggcactg gcaaatgctg tagccccttc cacatattcg
      361 cctgttgggg catcactcca aaactcgttg gcacggcata caatcccaaa catgtttgta
      421 tttttgccat actggtattt cacatttttt ctcactgggt cagtggctga ataaacacgc
      481 accttacacg tggttttatc gctacggtgg tattcgaact ccgcagcttt accaccggca
      541 tccattacac cgtagttgtt tttaatggtc catgtttcgt ttctctcgtc gtacacaccc
      601 ggacaagatt taaggaagtt aaagaactcg ctgaaagaac tgatatggca caacggatat
      661 atcataacag gattggtatt gcctttcacc acattgccaa ccacagtaat gccaatggaa
      721 aaaccggctt cgttgagtcc catcatgggg ttgagaaaat tcggatgctc attggtattg
      781 cttctgatac caagaaaagc ataagtatcg ggcttaccgt tgccatcgtg gtcggtatcg
      841 ggaattccat cgttgttggt gtcgttatcc cagaaaatca agcggttggt gcctgtccac
      901 atacgcaatt tccaatgaat ggggcggcta tcgtgggtga actcaccgat gataagtccc
      961 tcggtgcacg gacgggttaa caaaggtatt agcagaagag cagagaaaat taatcgtttt
     1021 atattcatcc tggcaaattt ttaataggtc attcgttcca attcgttgcc attagaatcg
     1081 tattccaccc actccccttt tctctcgccg ttttcgaaac gaccttgctc ctgaattttt
     1141 ccgttttcgt agtagaattt ccattcacct tcctgaacac cgtcaaccag atttccggtc
     1201 atttcgatat tgccgttggg gtaatacact ttccagatac ccgattccag gtcgttaaca
     1261 aaagtgcctt ccgacatcat cttgccatcc gtatcgaaat actgccagtg accatttttt
     1321 gcatcattgg cataatagcc aaccgattcc ggtattccgt tctggtaata cgatttccat
     1381 tcgttggttt ttattccctt ggtaaattta ccgcaatact gtaattgccc gttatcgtga
     1441 tacagcttcc attctccggt ttttacattg ttctctacca taccgatcga ctcgaggtga
     1501 ccgttttcgt gatagaattt ccattcgccg ctaacaacac cattttcgaa attgccgctt
     1561 gacataagtt gcccgttttt atgataaaat tcccatatac cggtttccat atcgttttcg
     1621 ttgtatgccc caaccgaaag caattcgccg gtttggtagt attttttgat aggttcgttt
     1681 tgtgctgtta aaccaatcga ggttattaca aaaagtagaa gcagatacag tgttttcata
     1741 ctaatttaat ttgatttcgc atatttactc aactaacaca aaggctgccc aatagtaggg
     1801 agggtatttt ttacgcattt cctgctgggt gtttacaaaa gcctgattca catcgagtgt
     1861 ctgggtaaga ttttgataga ggagggtcat aaattcagcg gtttctgtgt cggcaacttt
     1921 ccaaaggctc ataatcagat aatcggctcc tgccatttta aatgcccgct gcaaaccata
     1981 cactccctcg ctgcctttca catcgcccag gccggtttcg catgccgaca gcactaccag
     2041 tgaagtttta ttgaggtcaa ggcacgacac ttcgaaagcc gtaagaatgc catcaccttt
     2101 tccactgtag ggagggaatc cactccatgt agtattaccc ccggccatta tcagtcccga
     2161 tcgtaccata gggtttttcg acacagagaa acgaagacct tgctccatag taccataggc
     2221 agtctgtgga aaataaaaac cgtgggtggc aatgtgaatt acctgaacgg ggttgctact
     2281 gctctgggcc ttgaatatat cttcagtgcc attatcggca gtaataatct gatatgggaa
     2341 ggtgttttca tcgaaaattt taccaatctg ttctatttct tccagtgtgc cgggaaggta
     2401 tgtccagctt tcacccctag aattggatgg tatatatacc gaccgatttt tatttcgatt
     2461 tacaacagtg ctatcaatgt tgctttgctg cgattcgtcg aactgcatgt cgaaattgat
     2521 accgccgtac aggtatgccc agatttcatt gcctgctggc tttcggaacg aacttcgggt
     2581 ttcgtaagcc agcgaagcgg tagatgcaca atagtgcaag gtatatttat cgcagaggaa
     2641 aaggctatcg ttgtaaggta atgccgaaaa tgcaatactg tggagcatgc catcgggagc
     2701 ataatatact gttttaatgc cttgtaggta tttttccaaa ggctgccaga ggagcgagta
     2761 gagttgttcg gaataatcta cattacggta ggcagaatca acaaaggtga cacctctgtt
     2821 actaccgtac aatgcatcaa taataagaaa gttcgtttcg gagcccaatg ccagtaaaat
     2881 gcgttgcagt tcatcctcac tgaacatagg cagaagcaat ggctgttggc agtttttatc
     2941 aacgattaaa gcgcagtata tcaccgaatc agtagcatta tccacaatgg cagcatcgaa
     3001 attgtgccag ttgaagtgaa taaattctat ggcaacttca tcgtgtttta ggttcttttg
     3061 cacatctttc catgaataat cctgaatttt attaaaatcg gaaaacaact gtgaactttt
     3121 cacaagctcc ttttctatct cattggcttg ttgttccaac tggctggctt cttctccggc
     3181 ggcgtattta tcggcaatca tattttttgt taaaatccaa ttcttgtagt tttcgataag
     3241 ttgccggtcg cctgaattgt acactgcatt gcgcattgag gtggatgatt tcagaagtat
     3301 gcctttgtgc ttcataatat tgtcgtatgc catcgccgat aatccggggt tttgcaggtt
     3361 gcggaacaag caatacgaat agaaattcat aaaatctccg gaaagcgtat tgaaataatt
     3421 tgcctgttct tcttcggcca tggtggcaaa attcgacatc atcaacttat tttgatgttg
     3481 cagtatctga gggatatagc tatcagccgc attccagttt ttttcgtaaa ccgctatttt
     3541 attgagcatt ttcaggcaat cgtataacgc agtcggttcg tttttcttag cggcttcggt
     3601 ataggcttcg ttggcatatt tttttgcatt actaacatcc tgcatattca gatagctctg
     3661 tgcgagtccc aattttgcat cgtaatttcc ataaaaaacg cctgttttac tgctggtttt
     3721 tatactttta ttgtaatata ttatcgcgtt ttcgtaatca cccttattaa gataggcttt
     3781 ccccatgtaa ctgtatgtcc acgacaagct cacccggtca tccatttcac tggcataata
     3841 atatgcattc tcgagataat cgagggcagc atcggcatca ttattctcgc ttagagcata
     3901 atttccaagg ctttgatagg catttgccaa agggtaaaac aaatccctgc tttcggctat
     3961 ttcaagggcc tcatgcatac gtctaagagc catttccgca tccccctgat ctttgtaaat
     4021 acctgcaata ttaagcaagg agtacgccat atcatcatac tcattttttt cgcgttgcaa
     4081 ggccaatgcc ttttcgtggt attccagggc tttatctggt tccccatacc gttcgtaaat
     4141 ccagccaatg tgattatacg aaactgaact aagttcttta ccgttatgtt gtttttcaaa
     4201 ttgaatagct ttttcaaaat aggtcagggc ttccggcaaa tgaccaataa gttttaatat
     4261 ggttccatgt aaagaaaata catcaccata atagaaattg gggtagctcg tcaggttcga
     4321 ttccaggtct tcaacttcac gcaatgcagc ttctggtttg cctgttctga atagatattg
     4381 ggctctgagg tagctttctt ccattaagcg gtaattttcg gtagctggta atttaagttt
     4441 ttcgcattta tgcagatact ttcctgcagc cgaataatcg ggcatctgat aaatactgaa
     4501 catatataac ctgattaact gagtgcaagc atcgtgaatt gcaacaggct ctttcgattt
     4561 ttttgctcca gtcaacgcgg tttgcgcata cgaaaaagcg agatccggtt cttccggctc
     4621 tgtttcagtg taacataacg acagcatcag gttggcaaag ctttttgtaa tggtatagta
     4681 tgcataattc aatgcttcat cagcccattc tgctgcttca taatacctat cctcatcgta
     4741 gtaaatttga gctaatgcca aacagacatc agcgtattct tcttcaagga agtcttgttc
     4801 atcagaattt tcaataatac ttaatgtttt attcagtagt attttagctt tttcattctt
     4861 attagtttca tataaaatat cagcgatgtt gataagcaat tgcacataag tgggttcatc
     4921 tcctgcaata cgggcgcatg ccgataaata caataaagta tcgcaagctt gttcgtagtt
     4981 tttagcattg tatagttctt ttgattcatc ataaatacca cttacaaatt tttcgtaccg
     5041 tggaagccat gtttttatgt cagcattata gctatttgtg taagagttaa gagcatttgt
     5101 ttccatatac agatagttca taacatcata tgcaactttt tcgccaatca ggtcgttacc
     5161 tctccgatac attgcaatcg catcgttgaa tagtggcatt gcattctcat taatatccga
     5221 atcaattatc gcaagggcaa gcaacataag aaaactggcg ctgtcggaag aagaaactgc
     5281 ctcaggaaat atttctctga ataattgatc tttggccccg tcaacaaact tgttttcatc
     5341 atctaacaga tcaccaaagc ttcttgagta gaatgatgca tggtaatcga caatattaat
     5401 tcccgttttt ttgctcagca acacggcata tttgttcttt cccaggtctt tgcaaacctt
     5461 tgcaatattg atgtatgtat atgaaacgcc atacaaatct tcaggcattt tatcagcagc
     5521 catttcagcc ctcagcagaa atttaaaagc tttctcaggg tcagatgctt tttcagtctg
     5581 agcaagggta atatacgatt ttatggccct gttgtaatct tctgcctcat aatataaacc
     5641 ggcagctgcg agcagatagg tatttgcttt cattgtgtcg ccggcatagc attctacttc
     5701 gtataagctc ctgtaaattg atgcttctgc accagaatca cctgctttat tgtagtggtc
     5761 agccagtgaa agatatattg ctgcggcttc gtcgtattgg ttgttttcgg tataataccc
     5821 agccctgttt ttcgaattaa cgagcaggtt taatgtatcg ttttcttgct tgtaacacca
     5881 ttccagacta tcgaatatgc cgataataat ccggttgtat ttgtcgtaaa ttatgtcggg
     5941 atttaaaaat acggtatccg ttttgtaata cttcagg
//
LOCUS       DAOOQP010000149         5926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_16299_length_5926_cov_4.557316, whole genome shotgun sequence.
ACCESSION   DAOOQP010000149 DAOOQP010000000
VERSION     DAOOQP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16299_length_5926_cov_4.557316"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLK75_11730"
     CDS             complement(<1..545)
                     /locus_tag="PLK75_11730"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HOY39957.1"
                     /translation="MKTSIKLSIFIFTVFTVFFTSCGIKDNKPPRIYLIGSNDTTIIH
                     REKFVDPGYFAEDNVTHTENIILSNNRSEILDTNSDGYTTRHEIYEITYTATDEEGNS
                     KSVIRKITVNNISTPYAQSYNVIRRQSSFFGDTTYRATVTVDTRVSGRLKISKMYIHK
                     TPGTNNLIYYRVNADLWASDLV"
     gene            complement(614..3256)
                     /locus_tag="PLK75_11735"
     CDS             complement(614..3256)
                     /locus_tag="PLK75_11735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOY39958.1"
                     /translation="MKYIIIIFYLFLFTGQIAFSQVHLSPALRTACENKWNSDEKIGF
                     IIYLNEQVDNEALTEQLSKTSVVHGERATYIIAEHMNLAFKTQKNLLQYLYKHADDYP
                     DHITTIKPYWIRNAVYVEAIPSLVSELVKRDDIHCIDLNYGKFSVIEPIGRGENAPKN
                     LTGPEPGLIAINAPALWAMGYTGRNTVLLSMDTGVWTDHPALSEAFLGNYFPLSQVWF
                     GVRSAIPADNASSCHGTHTTGTVLGLDPATTDTIGVAYNASFIATDPVASSNSDLLAP
                     TDFMDVFQWVLDPDGNPETTYDIPDVINNSWGYDYTLAIPMGACEMEEAHVLETIEAA
                     GICSPFSAGNEGPAASTNGFPAMLAYNLVNCMSIGAVNGNNENYPIADFSSRGPTPCV
                     SEEGPLQIKPEVVAPGYNVRSASGHDTYEALSGTSMACPHVSGALLLLREAFPEASAY
                     ELKYALYVTAHDLGEPNEDNTYGMGMIDVLDAFNYLSGFYTPAPPVTNQVDITCELIT
                     PDVKVFCLDEAHFTPQMHIVNNGTSAVSDLVLKATCNDVVFFDAIPNLLLLPGQDATI
                     DLNEIHGMPGRNEIAVSATHSTEVDEYNVFDNYDVASFNVIGEYDASYIQCFDTLNGY
                     LVNADWFIDNPDSENSWNVTTWNDATSNRALFIDFYNYLPREGQLDDAWSPRVHVPDT
                     GSTSLSFVYAYKKRMETLFKDSLIVLVSTDCDGNFNSELWRIGGVDMATVSGNAGNNG
                     FTPQTEADWDTVTIGLNQFKNNDIVVCLRTRNDDGGTLYIDDFKIEVQSTSGIESPIQ
                     SKPYLAVYPNPATECLFIASNSDLHSSTITIYDTGGRLVFEIPAGTLAGAELGIGFLQ
                     PGMYFIQLDDGESLLQKRFVKLKQ"
     gene            3354..3950
                     /locus_tag="PLK75_11740"
     CDS             3354..3950
                     /locus_tag="PLK75_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39959.1"
                     /translation="MKKIIGFLLATVIILSGVAVHAQENEEGFSNSESGYTKGMGGPI
                     ISFSTIHGEFSVFMGGGGGVVLKNFILGVYGMGQASQIEDWHNSNRFVSIWHGGIWLG
                     YSFLPDKIVHPVIELNAGMGNFSTFAQNDYSSLYTDKTGVFVLSPKLGVEFTVSSFVK
                     IGVGGEYRYANFTKPNTSYSSKDISAPAGYITIKLGEF"
     gene            3966..4619
                     /locus_tag="PLK75_11745"
     CDS             3966..4619
                     /locus_tag="PLK75_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39960.1"
                     /translation="MQKFLLTISVLFVFNLVNAQESIYGIDFSNTAYPFNKWKKEVLD
                     MANTTKDVGYLTEEEKKLIFLCNLLRLDGKLFSQTFYEQYCHIYRIRDNEHTTSLKSE
                     LNKIKKLPCLEPKKDLCNVAEDFATKSGSSGKTGNKNYKSRYSKLSKSYGSITENYTS
                     GNAEAIKIIMTMLIDETNPSHINRKNLISKQSNCTGVSIKPHSKAGYNAVMNFAKTK"
     gene            4738..5016
                     /locus_tag="PLK75_11750"
     CDS             4738..5016
                     /locus_tag="PLK75_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39961.1"
                     /translation="MKKAKIYFWLKNAGLLSAIIVMLAGSTSCRSRRHATKYGPPPSD
                     YNNTITKYGVPADMDNQIDLPPVKDTGNNTITKYGVPVDKPDYKPDEE"
     gene            5059..>5926
                     /locus_tag="PLK75_11755"
     CDS             5059..>5926
                     /locus_tag="PLK75_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY39962.1"
                     /translation="MNLRRKLALKLHSHYVKTQAKLHILNYFFWECTAKCNLACLHCG
                     SDCKVQPNISDMPAADFLNIARQVALRYNPAEVMVVITGGEPLMRSDLHIVGAELMRI
                     GFAWGMVSNGYLFTEEKFRLLRENGLRSVTISLDGLEESHDWMRGKQGSFAKALNAIK
                     ILAAEPGMTYDVVTCVNQRNFGELDQIRKLLVGTGVKRWRLFMIDPIGRACENKELFI
                     SGDQFRQLMDYITALRRKGSLAVSYGCDGYLGEYESQVRDGFFFCRAGIHVASVLANG
                     DISACPNINRSFI"
BASE COUNT         1784 a         1189 c         1275 g         1678 t
ORIGIN      
        1 acaagatccg atgcccagag gtcagcatta actctgtaat aaattaagtt gttcgttccc
       61 ggggttttat gaatgtacat cttacttatt ttaaggcgac ctgaaactct ggtatcaaca
      121 gtaaccgtag cgcgataggt tgtgtcgcca aaaaacgaac tttgccgacg gataacgttg
      181 taagattgcg catagggggt tgaaatattg tttactgtaa ttttccgtat cacagacttc
      241 gaattacctt cttcgtcggt tgcagtatag gtaatttcgt aaatttcatg gcgggtggta
      301 tagccatctg aattggtatc gagaatttcg cttctgttgt tcgaaagaat gatgttctcg
      361 gtgtgggtaa cattatcctc ggcaaaatag ccgggatcca caaatttttc acgatgaatg
      421 atagtggtat catttgagcc tatcaggtaa atgcggggag gcttattgtc cttaattccg
      481 caggaagtga aaaatacggt gaatacagta aaaatgaaaa tgcttaattt gattgacgtc
      541 ttcatattta attaaattaa ttcaaaatgt aaaaatagtg aattttagta taaaacacaa
      601 tatctgtatg attttattgc ttcaatttaa caaaacgttt ttgcaacaag ctttccccat
      661 catccagctg aataaaatac atacccggtt gtaaaaaacc tattcccagt tcagcaccag
      721 cgagagtacc tgcaggaatt tcgaatacaa gccttcctcc ggtatcgtaa attgtgattg
      781 ttgaggaatg caaatcggag tttgatgcaa taaacaggca ctcggttgca gggttcgggt
      841 aaaccgcaag gtagggtttt gactgaatcg ggctctcgat acccgatgtg ctttgaactt
      901 caattttgaa atcatcgata tataaggtac ctccatcatc gtttcgtgtt ctgagacaaa
      961 cgacaatatc gttattttta aactgattga ggccaattgt tactgtatcc caatcggctt
     1021 ctgtttgagg tgtaaaacca ttgtttcctg cattgcccga cacggttgcc atatccacac
     1081 ccccgattct ccacagttcg gaattgaaat tcccgtcaca atccgttgaa actaaaacaa
     1141 tcagcgagtc tttaaacaat gtttccatac gctttttata agcatataca aaactcaggc
     1201 tggttgaccc tgtatcagga acatgaaccc gtggcgacca ggcatcatcg agttgccctt
     1261 cgcggggaag ataattatag aaatctatga ataaggctct gtttgaagtc gcatcattcc
     1321 atgttgtaac attccatgag ttttcggaat cggggttatc aatgaaccag tcagcattta
     1381 ccaaatatcc gttgagagtg tcaaaacact gaatataaga ggcatcatac tcacctatta
     1441 cgttaaagga ggccacatca taattatcaa aaacattata ttcatcaacc tcggtagagt
     1501 gagtagctga aactgctatt tcgttgcgtc ccggcattcc atgaatttcg tttaaatcta
     1561 ttgttgcgtc ttgtccaggc aacaagagca agtttgggat ggcgtcgaaa aaaacaacgt
     1621 cattacaggt tgctttcaat accaaatcgc ttacagcact ggtaccatta ttaacaatat
     1681 gcatctgtgg ggtaaagtgg gcctcatcca agcaaaacac tttcacatca ggggtaatta
     1741 gttcgcaggt aatatcaacc tggttggtta caggtggtgc aggcgtgtaa aagcccgaca
     1801 ggtaattgaa tgcatccagt acatcaatca tacccatacc ataggtatta tcttcgttgg
     1861 gttcaccaag gtcgtgtgca gtaacatata acgcatactt caactcataa gcactggctt
     1921 cagggaaagc ttccctcagc agcagcaggg ctcccgacac atgcggacaa gccattgagg
     1981 ttcccgacaa agcttcataa gtatcatgtc cggacgcaga gcgaacatta tacccaggag
     2041 ccacaacctc gggttttatt tgcagtggac cttcttccga aacgcatggt gtgggtcctc
     2101 tgctgctaaa atcggctatc ggataatttt cgttattccc gtttactgca cctatcgaca
     2161 tgcagtttac caggttatat gcaagcatgg ccggaaagcc attggttgat gctgcagggc
     2221 cttcgtttcc ggccgaaaat ggtgagcata tccctgctgc ttcaatcgtt tccagcacat
     2281 gtgcttcttc catttcgcat gcgcccatag gtattgccag tgtgtaatcg taaccccatg
     2341 aattatttat gacatccgga atatcgtagg ttgtttcggg attaccatcc gggtcgagta
     2401 cccattgaaa aacatccata aaatcggtcg gggcaagcaa atcggagtta ctggaagcaa
     2461 ccggatcagt tgcaatgaag gaggcattat aagctactcc aattgtatcg gtagtagccg
     2521 gatccaaacc aagtacagtt cctgtggtgt gggtaccatg gcaagaagaa gcattgtcag
     2581 caggtatagc acttcgcact ccaaaccaga cctgcgataa cgggaagtaa ttccccagaa
     2641 atgcttctga aagtgcaggg tggtcggtcc acacacccgt atccatgctt aaaaggactg
     2701 tgttgcgacc tgtataaccc attgcccata gtgccggagc attaatcgct attaacccgg
     2761 gctcaggacc cgtcaggttt ttgggagcat tttcacccct gcctatgggc tcaataacag
     2821 aaaacttgcc atagtttaaa tcaatacaat gtatatcatc gcgttttacc agttccgaaa
     2881 caagagaagg gattgcttca acatacactg cattgcgtat ccaataaggc ttgattgtgg
     2941 tgatatgatc agggtaatca tcggcatgtt tatagagata ttgcaacaga tttttctggg
     3001 ttttgaaggc caggttcatg tgttctgcaa taatataagt tgctcgttct ccgtgaacaa
     3061 cgctcgtttt acttagctgc tctgtaaggg cttcgttatc aacctgctcg tttaaataaa
     3121 ttataaagcc aattttttca tcggaattcc atttattttc gcaggccgtc cttagcgcag
     3181 gacttagatg cacctgtgag aaagcgattt gccctgtaaa gagaaagaga taaaaaataa
     3241 taattatgta tttcattgat ttaagtttta ggtaaaatta gtattattca taaagtagca
     3301 caaaaaatta atcaacattt taattgatta tatttgcata taaaatttcc ggtatgaaaa
     3361 agattattgg gtttttattg gcaacagtta ttatcctgtc gggagttgct gtgcatgctc
     3421 aggaaaacga agaaggtttc agcaattccg aatcgggata tactaaaggc atgggtggtc
     3481 ctattattag cttcagtacg atacatggcg aattttcggt atttatggga ggaggtggcg
     3541 gggttgtatt aaagaacttt atactcggcg tttatgggat ggggcaagct tctcaaatcg
     3601 aagactggca taattccaac aggtttgtaa gcatttggca tggaggcatt tggctcggat
     3661 attcttttct tcctgacaaa atagtgcatc ctgttattga attaaacgca ggaatgggga
     3721 atttcagcac atttgctcag aacgattaca gctcccttta taccgataaa acaggagtat
     3781 ttgttctatc cccgaaactc ggcgtagaat tcactgtctc cagttttgta aaaataggcg
     3841 ttggtggtga ataccgatat gcaaatttca caaagcccaa caccagttac tcatcaaaag
     3901 atatttcagc tccagccgga tatataacaa ttaaacttgg agagttctag ctcactaatc
     3961 gatttatgca aaaatttctt ttaaccattt cggtattatt cgttttcaac cttgttaatg
     4021 cacaagaaag tatttacggg atcgatttca gcaacactgc ttatcctttc aacaaatgga
     4081 aaaaggaagt acttgatatg gcaaatacga caaaggatgt tggttatctt accgaagaag
     4141 aaaaaaaact aatttttttg tgtaatcttt tacgactcga tggtaaactt ttcagccaaa
     4201 cattttacga gcaatattgc catatttacc ggataagaga taatgaacat acaacctcgc
     4261 ttaagtccga gcttaataaa attaaaaagc ttccctgcct tgaacccaaa aaagatcttt
     4321 gcaatgtagc tgaggatttt gctacaaaaa gcggaagcag tggtaaaacc gggaacaaaa
     4381 attacaaatc gcgatattca aagctaagca aaagctacgg gagcatcacc gaaaattata
     4441 cttcggggaa tgcagaggca attaaaatta taatgacaat gctgattgat gaaaccaatc
     4501 catcacatat caacaggaag aacctgattt cgaaacaaag taactgcacg ggagtcagta
     4561 taaaacccca ttccaaagca ggttataatg cagtgatgaa ttttgcaaaa acaaaatagt
     4621 agttccacat agtaatattg aacgtttctg gggagggtaa taatggtttc agtgtaaaat
     4681 attttttatg gaatattgtt ttttttgtat cttcacaaag aaaaactact ggtcaatatg
     4741 aaaaaggcaa aaatatattt ctggctcaag aatgcaggtc tgctttctgc aattatcgta
     4801 atgttagcgg gatccacttc atgcaggtcc cgaaggcatg caaccaaata cgggcctcct
     4861 ccctccgact acaacaatac aataaccaaa tacggggttc ccgctgatat ggacaaccag
     4921 attgacctgc caccggtaaa agatacgggg aacaacacga taaccaaata tggagtacct
     4981 gttgataagc ccgactataa acccgatgaa gagtaataat tggggtcagg cgcaccgata
     5041 taacaagttg actatttaat gaatttacgc cgaaaactgg ctcttaaact acattcgcac
     5101 tatgtaaaaa cacaggctaa attgcacata ctgaattatt ttttttggga gtgcacagcc
     5161 aaatgcaatc ttgcctgtct gcattgtggc agcgattgca aggtgcaacc gaacatttcc
     5221 gatatgcctg ctgcagactt cctgaacatt gcacgacagg tagcattgcg ctataaccct
     5281 gctgaagtaa tggtagttat tacgggaggt gaaccactga tgcggtccga cttgcacatt
     5341 gtgggcgcag aactcatgcg aataggcttt gcatggggaa tggtgagcaa cggctacctt
     5401 tttactgagg aaaaattcag actgttacgc gaaaatggct tgcgctcggt taccataagc
     5461 ctggatggat tggaagaaag tcacgactgg atgcgaggaa aacaagggtc gtttgcaaag
     5521 gctttgaatg ccattaaaat tctggccgcc gaacccggca tgacttatga cgtggtgacc
     5581 tgtgtaaatc aaagaaattt cggagagctt gatcaaataa gaaaactgct tgttggaacc
     5641 ggtgtgaaac gatggcgatt gttcatgata gatcctatag gcagggcatg cgaaaacaaa
     5701 gaattattta tttcaggaga tcagtttcgt caattaatgg attacattac cgccctgcgc
     5761 cgcaaaggga gcctggctgt atcatacggg tgcgatggct atcttggtga atacgaatca
     5821 caggtacgcg atgggttttt cttctgtcgt gccggaattc atgtggcttc ggtacttgcc
     5881 aacggagata taagtgcttg cccgaatatt aaccggagtt ttattc
//
LOCUS       DAOOQP010000150         5846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_16581_length_5846_cov_4.476256, whole genome shotgun sequence.
ACCESSION   DAOOQP010000150 DAOOQP010000000
VERSION     DAOOQP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16581_length_5846_cov_4.476256"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /gene="nrfA"
                     /locus_tag="PLK75_11760"
     CDS             <1..187
                     /gene="nrfA"
                     /locus_tag="PLK75_11760"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formate-dependent reduction of
                     nitrite to ammonia; cytochrome C552; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOY39963.1"
                     /translation="GYNEEVPMPDISTKEKAEQYVGLDSQKLKTEKEEWKVKVLPQWL
                     SKAEERESKMPEPKRIK"
     gene            421..1401
                     /locus_tag="PLK75_11765"
     CDS             421..1401
                     /locus_tag="PLK75_11765"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOY39964.1"
                     /translation="MIWLSSAEAALASVLCFLLVIILMAIIPQNISSETIGAKIGYSN
                     IVFTWYYALSVFFLLTVLALTSMRRFLPCNFRNMMFFLNHSGMFIALCAAVLGQADKK
                     QLYMQVPVNTTVWYAENEDGYSVELPFALHLSKFVVEYHPRKVALISHDGQLYKTKGN
                     AVAEAVRGNSFFIGPYRIRVFNEPDSLNSILLKVSKPDKTTMEFSFEHLNDPQTPLFA
                     KISNDTIITLLPAEPSYFGSEALLYTKSGKVAEPVTIAVNKPLKTEGWDVYQHSYNNN
                     TETEPGYSVLYLVYDPWLPVVYIGIALMLAGAVLMLFARKKNEIKHNDLE"
     gene            1388..2191
                     /gene="ccsA"
                     /locus_tag="PLK75_11770"
     CDS             1388..2191
                     /gene="ccsA"
                     /locus_tag="PLK75_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOY39965.1"
                     /translation="MIWNSLIWFVLIFLLCWGIGIVMLYRKNNPDRLPLVAKLAPVAG
                     NISLLLFVIILWVQLDRPPMKTMAETRVLYSLFVSWITWFIFLTTKSKWMYLLGMVMS
                     LVFLIVDVLNPEYQSKNLMPALQSPWFIPHVVIYMIAYAVMAAACLSAVKGLVNLYKG
                     KNDADSDINLAMKLVYPGMALLTTGMLLGAIWAKIAWGNYWTFDPKETWALLTWLFYL
                     FTIHLHYACPKRKKLLLWILGLSFIVLIITWMGIRFLPSAMQSIHVYGG"
     gene            2273..3142
                     /gene="lipA"
                     /locus_tag="PLK75_11775"
     CDS             2273..3142
                     /gene="lipA"
                     /locus_tag="PLK75_11775"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOY39966.1"
                     /translation="MMKTLHKPQWLNKKMDFESVAEVTRMLRTLKLHTVCEGARCPNI
                     SECFARSTATFMILGDVCTRNCSFCAIPQSKPLPPDPDEPRHVAEAAGQLALRHVVVT
                     SVTRDDLPDGGAMQFALTIAEIKKRLPSATIEVLIPDLKGVEASINMIIDAKPHIINH
                     NMETVPSVFKRVRPMGNYATSLSLLKYVKSQDAAIITKSGIMVGLGEIPDEVEHVMDD
                     LRRCGCDVMTIGQYLPPSSNHALLREYVTPEQFESYKIIALGKGFLYVASSPYVRSSY
                     NAAEDMDLIKKQL"
     gene            3145..4122
                     /locus_tag="PLK75_11780"
     CDS             3145..4122
                     /locus_tag="PLK75_11780"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOY39967.1"
                     /translation="MKRISANNLDAAFWFAVEEYIMNNPAFTDEYFMLWRTVPTLMIG
                     RFQNTLHEINSDFVTENNVQVVRRNSGGGTIYTDPSAWQFSFITWKNQGEAKDFQMFT
                     KPVIEALKQLGVPAEFNNRNDILAVNKKFSGNAQFSTRDRFLHHGSILYDTNIDNLVM
                     ALHVDDEKFISKGIKSVRERVTNIRPFMTTDMTSEEFSKAMNRLLASEMEECVLSEKD
                     LQVIENIRTSKFNSWEWNYGHSPEFNMKKSQRFEGGKVEVNLLVKSGKILNCSIQGDF
                     FAHIDIGKLEKSIEGLRHHPDDVLGVLQKSYDNGIIYRVSINELASLFF"
     gene            complement(4130..4744)
                     /locus_tag="PLK75_11785"
     CDS             complement(4130..4744)
                     /locus_tag="PLK75_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOY39968.1"
                     /translation="MFLETVKIVNGELVNFDLHLMRMAKTLNYHYSAFYTATLLKNLS
                     IAAQQIAVSQCSEAIVFKMRIVYTCNDYSISADPYFAKSVQSLKIVHNDAIDYSFKYE
                     DRSRLTSLLQLRENCDDILICKNGFITDTSFSNIVFKKYGSYFTPSHFLLNGIKRMQL
                     VSNGFIKEKEITLHNIKEYESFNLINAMLDLNIYNEHNINNIEL"
     gene            complement(4735..5730)
                     /locus_tag="PLK75_11790"
     CDS             complement(4735..5730)
                     /locus_tag="PLK75_11790"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOY39969.1"
                     /translation="MEINGVQNVIEKMNQWGYESKPFVFAFDFELKQAFIIDNPLLQN
                     EVLFRTPESNNFSKADNRKKNNPKKLQVIDFIDIHDYETKFRKVHNAIKHGDSYLCNL
                     TVKTRISLNISLTELLLQSWSYYGINYKNQFVSFSPERFVYIKDRTILSNPMKGTIDS
                     ISEEAYLQLANDYKEDCEHNTIVDLIRNDLGMVASNISVRRFKYIEPLYTDRGHLLQM
                     SSEIEGTLPENYQSQLGNIIFTLLPAGSVSGAPKQSTLKIIRESEGENRGYYTGVFGY
                     FNGKSLDSAVMIRFIENDYGRFYYRSGGGITINSQCKSEWQESMNKIYIASSPCF"
BASE COUNT         1734 a         1114 c         1205 g         1793 t
ORIGIN      
        1 gggatataat gaggaagtac ctatgccaga tatcagcaca aaagaaaagg cagaacaata
       61 cgtaggcctc gattctcaaa aactaaaaac cgagaaggag gaatggaaag ttaaagtatt
      121 gccgcagtgg ctttctaagg ctgaagaacg ggaaagtaaa atgcctgaac ccaaacggat
      181 aaaataataa aaccccatgc ttctgctgcc atttcgctct aacggatgcc ctcaacgtcg
      241 atgtcactta aaagaatttt aataatagcc agcttcgtat ttgttttggg cgtggttttt
      301 caactgttgt taggaccggt caatattagc ctactcaaat acccggtaaa tatggttgtc
      361 gggttgcaat tcttggtgat tataacctca atgcatcttt tcctgaaaaa aacgaaggtc
      421 gtgatctggc tctcttcagc tgaagcagca ctcgcttcgg ttttgtgttt ccttctggtg
      481 attatactta tggccattat tcctcagaac atatcatcag aaactattgg tgcaaaaata
      541 ggatatagca atatagtttt cacctggtat tatgcgcttt cggtgttttt tttgcttacc
      601 gtacttgctc tcacaagtat gcggcgattc ctgccatgta atttcaggaa catgatgttt
      661 tttttgaatc attcaggaat gttcattgcg ctttgtgctg ccgtactggg tcaagccgat
      721 aaaaaacaac tgtatatgca ggtgcctgtt aataccacag tatggtatgc cgagaatgaa
      781 gatgggtaca gcgtagagct acctttcgct ttgcaccttt caaaattcgt agttgaatat
      841 cacccaagaa aagttgcttt aataagccat gatggccaac tatataaaac gaagggcaat
      901 gctgtagcgg aagcagttcg tggtaatagt ttttttattg ggccataccg gattcgggta
      961 tttaatgagc cagattctct gaacagcatt ttgctcaagg tgtcgaagcc cgacaaaaca
     1021 actatggaat ttagttttga gcatttgaac gatccacaga ctccgctctt tgcgaaaata
     1081 agcaacgata caataattac cttattgcct gcagaaccat cttattttgg ctccgaagcc
     1141 cttttataca caaagtcggg gaaagtggca gagcctgtta caatagcggt aaataagccg
     1201 cttaaaacag aagggtggga tgtgtatcag cacagttaca acaacaacac agaaacagag
     1261 cccggctatt cagttttata ccttgtatat gatccgtggc tgccggtggt atatatagga
     1321 attgccctta tgctggcagg ggctgtgttg atgttgtttg cacgaaagaa aaatgaaata
     1381 aaacacaatg atctggaata gtctgatatg gtttgtactt atttttcttc tgtgctgggg
     1441 cataggaatt gtaatgctgt atcgtaaaaa taatccggat cgtctccctc ttgtcgctaa
     1501 gctggctcct gtggcaggaa atattagtct cctccttttt gtaataatcc tctgggtgca
     1561 acttgaccgt ccaccgatga agacgatggc agaaacacgt gttctgtact cattgttcgt
     1621 gtcgtggata acatggttca tttttcttac aacaaaaagc aaatggatgt atttgctggg
     1681 gatggttatg tcgctggtgt ttttgatagt agatgttcta aatccggaat accagagcaa
     1741 aaaccttatg cctgccttgc agagtccatg gttcattccc catgttgtta tttatatgat
     1801 tgcatatgca gttatggcag cagcctgtct ttcggctgta aaaggcctcg tgaatttata
     1861 taaaggaaaa aatgatgcag atagtgatat aaacctcgca atgaagctcg tttaccccgg
     1921 aatggcattg ctcactacgg gaatgctttt gggtgctatt tgggcaaaaa ttgcgtgggg
     1981 aaattactgg acattcgacc caaaggaaac ttgggcttta ttgacatggc tgttttattt
     2041 atttaccatt catctgcatt atgcatgccc taaacgtaaa aaacttcttc tttggattct
     2101 gggattatcc ttcatcgtgc tcataattac atggatggga attcgttttt tgccgtcggc
     2161 tatgcaaagc atacatgttt acggcgggta aacgtgagtt ttgtcaggag gctcaattcc
     2221 ttatttaaca aaaaagattt cctacatttg caactacatt tgatttatta atatgatgaa
     2281 aaccctgcat aaaccacaat ggctgaataa aaaaatggat tttgaaagcg ttgcagaagt
     2341 tacaaggatg cttagaacac tgaaacttca tacagtatgt gaaggagccc gttgcccaaa
     2401 tattagtgaa tgctttgccc gaagcactgc aacatttatg attttgggcg atgtttgcac
     2461 acgaaactgc tctttctgtg ctatcccgca aagcaaacca ttgccccccg atccggatga
     2521 accccgtcat gtggcggaag ctgccggtca gctagcacta cggcatgttg ttgttacatc
     2581 ggtaacccga gatgacttac ccgatggcgg tgccatgcag tttgctttaa caattgcaga
     2641 aattaaaaaa cgccttccct cagctactat tgaggtattg atacccgatt taaagggggt
     2701 tgaagcaagt attaacatga taatcgacgc aaagcctcat attattaatc ataatatgga
     2761 gactgtccct tctgttttta aacgggtccg gccaatgggc aactatgcaa cttcgctctc
     2821 gttgctaaaa tacgttaaat cgcaggacgc tgcgataata acaaaatcag gcattatggt
     2881 gggccttggt gagattcccg atgaggtgga acatgttatg gatgatttgc gcagatgtgg
     2941 ctgcgatgtt atgacaatag ggcagtacct gcctccaagc agcaaccatg cattgttgcg
     3001 cgaatatgtc actcctgaac aatttgaaag ctacaaaata attgccctgg gcaaaggatt
     3061 cctttacgtt gcatcatcgc catacgtcag aagcagttac aatgctgccg aagatatgga
     3121 tttaatcaaa aaacagttat aaacatgaaa agaattagtg ccaacaatct cgatgctgca
     3181 ttttggtttg ctgtggaaga gtatataatg aataaccctg cctttaccga tgaatatttt
     3241 atgctttgga gaacggtgcc aacacttatg attggtaggt ttcagaatac cctgcatgaa
     3301 atcaacagcg attttgttac tgaaaacaat gtgcaggtag ttagaaggaa tagtggtgga
     3361 gggactatct ataccgaccc atcagcatgg caattcagct ttattacatg gaagaatcaa
     3421 ggggaagcga aagattttca gatgttcacc aaacctgtga tcgaagcact caagcagctt
     3481 ggcgtaccag ccgaattcaa taaccgcaac gatattctgg cagttaataa aaaattctca
     3541 ggtaatgccc agttcagcac ccgtgaccgt tttctgcatc acggttcaat actctacgat
     3601 accaatatcg ataatcttgt aatggcactg cacgttgacg atgaaaaatt tatttcaaag
     3661 ggcataaaat cagtaaggga gcgggttact aatatccggc catttatgac aaccgatatg
     3721 acaagcgaag aattcagcaa agcaatgaac cgcctgcttg catccgaaat ggaggaatgt
     3781 gtgttaagcg aaaaagattt gcaggtaata gagaacatcc gtacttcaaa atttaattca
     3841 tgggaatgga attacgggca ctcaccggaa tttaatatga aaaaatcgca acgattcgaa
     3901 gggggcaagg tcgaagttaa tctcttggta aaaagcggaa agatattaaa ctgtagtatt
     3961 cagggtgatt ttttcgctca tatcgatatc ggcaagcttg aaaaaagcat tgaaggccta
     4021 agacatcatc ccgacgacgt acttggagtg ttgcagaaat cgtatgacaa tggtataatc
     4081 tatagagtaa gtattaacga attagccagt ttgttttttt aaaatttgac tatagttcta
     4141 tattattgat attgtgttcg ttgtatatat ttaaatcaag catagcatta attaaattaa
     4201 agctttcata ttccttgata ttgtgtagtg ttatttcttt ttcttttata aaaccattcg
     4261 aaaccaactg catccttttt attccgttca gaagaaagtg tgatggggta aaataactac
     4321 cgtatttttt gaacacaata ttgctaaatg aggtgtctgt aataaatccg tttttgcata
     4381 tcagtatgtc atcgcagttt tctcttagct gcaacagact cgtcaaccgg gagcggtctt
     4441 catatttaaa actgtagtca atagcatcgt tgtgtactat ttttagagat tgtaccgatt
     4501 tcgcaaaata aggatcggca gatatagagt aatcattgca agtgtaaaca atcctcattt
     4561 tgaaaacaat cgcttcactg cattgagaca cagctatctg ttgcgctgca atacttaagt
     4621 tcttcaatag ggttgcagta taaaaagcac tgtagtgata attcagcgtt ttcgccatgc
     4681 gcatcagatg taaatcgaag ttaaccaatt caccattcac tatttttacg gtttctaaaa
     4741 acatggcgaa gatgcaatat atattttatt catggattcc tgccactcgc ttttgcattg
     4801 gctgtttatg gttattcctc caccgctacg atagtaaaac ctgccataat cattttcgat
     4861 aaaacgtatc attactgcag aatcgaggct tttaccgttg aaatacccaa aaacccccgt
     4921 gtagtaacca cggttttcgc cctcgctttc gcgaataatt tttagcgttg actgctttgg
     4981 cgctcctgaa actgaaccgg ccggtaacaa agtaaagatt atattcccca attgactttg
     5041 atagttttca ggcagcgttc cctcaatttc tgaactcatt tgcaataagt gtccccggtc
     5101 ggtatataat ggttctatat atttaaagcg cctgaccgaa atatttgatg caaccattcc
     5161 aaggtcgttt ctaattaaat cgacaatggt gttgtgctca caatcttctt tgtaatcgtt
     5221 agccaattgc aaatatgctt cttcgctaat tgaatcgata gtacctttca ttgggttcga
     5281 taagattgtt ctatccttaa tatacacaaa cctttcgggt gaaaacgaca caaattggtt
     5341 cttgtagttt ataccatagt acgaccaact ttgcaacaga agttctgtca ggctgatatt
     5401 cagagaaatc cgtgttttaa cagttaaatt acacaggtag ctatcaccat gtttaatggc
     5461 attatgcacc ttcctgaatt tagtttcata gtcatgaata tcaataaaat caataacctg
     5521 cagcttcttg ggattatttt ttttgcgatt atcggctttt gagaagttgt ttgattcagg
     5581 ggtacgaaac aatacttcgt tttgcaacag aggattatcg ataataaatg cttgctttaa
     5641 ttcaaaatca aatgcaaaaa cgaagggttt ggactcatac ccccactggt tcattttttc
     5701 gataacattt tgtacgccgt taatctccat ggtttggagt tttgtgtatt tgcaaatgta
     5761 acgaaataat aaatttgtgg taattgtttt gagaaactgt tttgaatttt ttattttaat
     5821 ctattaagca ttaatttggt cattgc
//
LOCUS       DAOOQP010000151         5824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_16660_length_5824_cov_4.397296, whole genome shotgun sequence.
ACCESSION   DAOOQP010000151 DAOOQP010000000
VERSION     DAOOQP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16660_length_5824_cov_4.397296"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="PLK75_11795"
     CDS             complement(<1..1486)
                     /locus_tag="PLK75_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39970.1"
                     /translation="MTRNLIIALIIFGIPALSFGQLRFIENKNQWEPHILYKMKLSNG
                     SMFLEKDCITFNFINREEHDHSFAHHGATEENHQETIDGHAYRVTFIGANNDVKVESS
                     LPSSDYLNYYIGNDPSRWAGNVKQYQEILYHNLYDNIDLKLYITDKGLKYDFIVHPNG
                     DVDDIVLEYEGAGSLTINNNNLVVTTTVNDVVELQPLVYQIIEGEQVAIPCIYNIKKN
                     RVNYKLDRGYDTERTLVVDPSLVFSTYTGSTGDNWGFTATWDFSDNVYSGGIVFDVGY
                     PTTLGAYQVNFAGGTPPIPNSTYYALGCDVGIIKYNENGTQRLFATYLGGSGGQEMPH
                     SLVVTEENDLVVMGTTGSPDFPTTAGAYDNTFNGGDSVVYDNVVAFSNGVDIYVAKFS
                     STGSSLMGSTYVGGSGNDGLNFKQYYAQNNPVYNINWTMMHGNDSLYFNYADGARGEV
                     ICDSKGDIYVATNTFSMNFPQGINNGFQTSSGGKQDGIVFKLKGN"
     gene            complement(1605..3056)
                     /locus_tag="PLK75_11800"
     CDS             complement(1605..3056)
                     /locus_tag="PLK75_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOY39971.1"
                     /translation="MVTFIGSIVALIAGYFLYGRFIDRFFGIEENRVTPAIELNDGVD
                     YIPMPTWKIFVIQFLNIAGLGPIFGAILGAAFGPAAYIWIVLGCIFMGAVHDYFSGML
                     SIRHKGASLPELVSTYLGKGAKNFLRVFTLFLLIFVGVAFVSGPAGLLESLTGGGYQI
                     WLFVIFVYYILATLLPIDKIIGKIYPFFGILLLVMAVLIGGVIIIKATNGSMPMTELS
                     AAGMVNHHAQKTTTFIFPMMFIVISCGAISGFHATQSPMMARCLKNEKHGRIAFYGAM
                     IAEGIVTIIWATAAMNYFGTATDLNTTLASHNPAWIVHEISVTWLGKFGAILAILGVI
                     ACPITTGDTAFRSARLTIADMFNFSQTSINKRLVVSVPLFALGFLLTFAFNKQFAVVW
                     NFVGISNQILAAIVLWTAAMYLAMKRKFHWIISVPALFLSFVCFTYLLCAPFKTGGFN
                     IPLQYSYPVALFLSVITGVAWSLKLRKNRSRLK"
     gene            complement(3141..3911)
                     /locus_tag="PLK75_11805"
     CDS             complement(3141..3911)
                     /locus_tag="PLK75_11805"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOY39972.1"
                     /translation="MKTKQEIVENWLPRYTGKPLDSLGKHILLTNFQLYVELFAELCN
                     VPVEGLNKNMPSATSDDITIINFGMGSPNAATVLDLLSAIHPKAVLFLGKCGGLKKKN
                     ELGDLILPIAAIRGEGTSNDYLPAEVPALPAFMLQRAVSSAIRDFGKDYWTGTVLTTN
                     KRVWEYDERFKKYLQKTRAMAIDMETATIFIVGFANEIPTGALLLVSDQPMISTGIKT
                     SESDKEVTLRYVKDHVQIGINALEMIKSENQSVKHLRF"
     gene            complement(4023..4784)
                     /locus_tag="PLK75_11810"
     CDS             complement(4023..4784)
                     /locus_tag="PLK75_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39973.1"
                     /translation="MSKNIIAILLRSAMLTAIILTLSASVLRAQYYVIAGYNANCPLK
                     MDNLNLVVDYYNWEHPNLQETMEHFYYMDGFTCAIGTAYEPLFAEIGYTGATQTVSSG
                     MYDSGFWVEKQLRARLRTFDINIGICTGLKEYNYFAAGIGMNVGVFSVKTKTKADYIN
                     NDKWSGNLAKNESGVMTFTFFLRYSPYGGYIWLQPYISIAPNKFLGFDMNRNMHPVDQ
                     YLNPTTVPTHQQDLNITFKSTGVKMCILIPYYDNQ"
     gene            4933..>5824
                     /locus_tag="PLK75_11815"
     CDS             4933..>5824
                     /locus_tag="PLK75_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOY39974.1"
                     /translation="MRKKLFMLYGAVSLLAGCILWYVFCLPEPLFSDPVSIVLLDNRN
                     EILGAQIASDQQWRFPYKNDVPEKYRKCLICFEDKRFYSHAGFDPIAFVRAMVQNVST
                     GEKKSGGSTITMQVIRLQRKGKVRSVGEKIIETIMATRLELGYSKDEILALYASNAPF
                     GGNIVGLDAASWRYFGRTPDKLSWAESAMLAVLPNSPSLIHTGKNRELLKLKRNRLLK
                     KLYLNHEIDSLTYVLALDEDIPAHPYPYPMHARHLVGRANSEIPNSRRLGPMITTINK
                     EIQEKVLAIATTHQRSLAHNE"
BASE COUNT         1726 a         1228 c         1179 g         1691 t
ORIGIN      
        1 ggtttccttt tagcttaaat acaataccgt cctgttttcc gccagagctt gtctgaaacc
       61 cattatttat tccctgaggg aaattcatgg aaaatgtatt tgtggcaacg taaatatcac
      121 ctttgctgtc gcagataact tcaccacgcg caccatcggc atagttaaaa tacagtgagt
      181 cgttaccatg catcattgtc caattaatat tgtacaccgg attgttttgt gcgtaatatt
      241 gtttaaaatt cagtccatca ttaccacttc ctcctacata agtgcttccc atgagagagg
      301 atccggtgct gctgaatttt gcaacataaa tgtcaacgcc gtttgaaaag gctactacgt
      361 tatcatatac tacactgtcg ccgccgttga aagtgttgtc gtaggctccg gcagttgtcg
      421 ggaaatcggg ggagcctgta gtccccatta ccacaagatc gttttcttcc gtgaccacaa
      481 gactgtgtgg catttcctgc ccgcctgagc cgcccaaata agtcgcaaat aggcgctgtg
      541 tgccgttttc gttatattta attatcccta catcacaacc caaagcgtaa taagtggaat
      601 tgggaatagg aggtgtgccc cctgcaaaat taacctgata tgctcccagt gtggttgggt
      661 aacccacatc gaatacaatt ccacctgaat acacattatc gctgaaatcc catgtggccg
      721 taaaccccca gttatcgccc gtgctccctg tgtaggtgct aaaaacaagt gatgggtcaa
      781 ctaccagagt ccgctctgta tcgtagcccc ggtcaagttt gtagtttacc ctgttttttt
      841 ttatattata aatacatggt atggcaacct gttcgccttc aattatctga tataccaaag
      901 gctgcaactc aaccacatcg tttaccgtag tagtcacaac caggttatta ttgtttattg
      961 taagacttcc cgcaccttca tactccagca caatgtcgtc gacatcgcca ttggggtgca
     1021 caatgaaatc atatttcagg cctttgtctg tgatataaag ttttaaatcg atgttatcat
     1081 acagattatg atacaaaatc tcctgatatt gctttacatt tccagcccat cgcgaagggt
     1141 cattcccaat ataataattc aggtaatcgc ttgaaggaag agagctctca actttaacat
     1201 cgttgtttgc accgataaat gtcacacgat aggcatgccc gtctattgtc tcttgatgat
     1261 tctcctcggt agctccatga tgagcaaatg aatggtcgtg ttcttcgcga ttgatgaagt
     1321 taaaagtaat gcagtctttt tcgagaaaca tcgatccatt cgataatttc atcttataaa
     1381 gaatatgtgg ttcccactga tttttatttt cgataaaacg tagttgccca aacgaaaggg
     1441 caggtatccc gaaaataatt agtgcaatta ttagatttct cgtcatgttg attacagaaa
     1501 caaatcacta aaattagttt gtcaatttac aaaaatcaaa gggaaaaaac aaataaatta
     1561 taaaatgcta aaggatgagg gaattaattg ttttgataaa atatctactt aagcctgcta
     1621 cgattttttc ttaatttaag cgaccatgct acaccggtaa taactgacaa gaacagggca
     1681 acggggtagc tgtattgaag cggaatatta aatccgcctg ttttaaaagg tgcacaaagc
     1741 aagtatgtaa aacaaacaaa gctgagaaac aacgccggga ctgaaataat ccaatgaaat
     1801 tttcttttca ttgccagata cattgccgct gtccatagca caattgcagc cagtatttga
     1861 tttgagatgc ccacaaagtt ccagactact gcaaattgtt tattgaatgc aaatgtgagc
     1921 aggaatccta atgcaaataa cggtacgctc acaacaagtc gtttattaat tgaagtctgg
     1981 ctgaaattaa acatatcggc aattgtgagt ctggcgctgc gaaaagcggt gtctccggtt
     2041 gtaatagggc atgcaataac accaagaata gctaatatag cgccaaattt gcccagccaa
     2101 gtaactgaaa tttcatgcac tatccacgcc gggttgtgac tagccagcgt tgtgtttaaa
     2161 tcagttgctg taccgaaata attcatcgct gcggtagccc agataattgt aactatacct
     2221 tccgcaatca tggcgccata gaatgctatg cggccatgtt tttcattttt taagcaccgt
     2281 gccatcatcg gtgactgcgt agcatgaaat cctgaaatag caccacacga aatcacgata
     2341 aacatcatcg ggaaaataaa ggttgtcgtc ttctgggcat gatgattaac catacctgca
     2401 gcagacaatt ctgtcatggg catgctcccg tttgtggctt taataatgat taccccacct
     2461 ataagtactg ccataaccag aagaagaatt ccgaagaatg ggtatatttt cccgatgatt
     2521 ttgtcgattg gaagtaatgt ggcaagaata taataaacga aaataacgaa aagccatatt
     2581 tgataaccgc caccggttaa cgattcgagt aacccagcag gaccactgac aaatgcaact
     2641 ccaacaaaaa tcagtagaaa tagagtgaaa accctgagaa aatttttcgc gccctttcca
     2701 agatatgtgc ttacgagctc tggtaaactg gctcctttgt ggcgtatcga tagcattccg
     2761 gaaaaataat cgtgcacagc acccatgaaa atacagccca acacaatcca gatataagct
     2821 gctggcccga aagctgcccc gagaattgct ccgaaaatgg gacccagccc ggctatattt
     2881 aaaaactgaa ttacaaatat tttccatgta ggcatcggga tgtaatcaac cccgtcattt
     2941 aattctatag caggggttac tctgttttct tcgataccaa agaaacggtc gataaaacgc
     3001 ccatacagaa aatatcctgc tatcagagct acaatacttc caatgaatgt aaccattgct
     3061 caatagaaaa taaagcacta aaaataatcg aatttgattt taataattat gatttctgtt
     3121 atgtttagcg atgattgttg ctagaaccgt aaatgtttta ccgattggtt ctcgcttttt
     3181 atcatttcaa gggcattgat accaatttgt acatggtctt taacatagcg cagcgttact
     3241 tccttgtcgc tctccgatgt ttttattccg gtcgaaatca tgggctgatc cgataccaac
     3301 aagagcgctc cggtaggtat ttcattggcg aacccgacaa taaatattgt tgctgtttcc
     3361 atatcaatgg ccatagctct ggttttctgc aggtattttt taaaacgctc atcgtattcc
     3421 caaactcgtt tatttgtagt aagcacagtt cccgtccagt aatctttacc aaaatcacgt
     3481 attgccgatg aaaccgcccg ttgaagcata aatgccggta aagcgggaac ttcggccggc
     3541 agatagtcgt tagaagtacc ttctccacga atggctgcaa taggtaatat caaatcgccc
     3601 agttcatttt tcttttttag tccaccacac ttgccgagaa aaagcactgc ttttgggtga
     3661 atagcactta ataagtcaag aactgtagcc gcatttggac ttcccattcc gaagttgatt
     3721 atcgtaatgt cgtcgcttgt ggcactgggc atatttttat tcaaaccttc aacaggtaca
     3781 ttgcaaagtt cagcaaaaag ctcaacatag agttgaaaat ttgtgagcaa gatatgttta
     3841 cctaaactgt cgagaggctt tccggtataa cggggtagcc agttttctac gatttcctgt
     3901 ttcgttttca ttgccatgta atttgagcaa atgtaataaa ttgttgtaaa taatttgcca
     3961 tcgcagatga tttgcctcca ttcggaatga aatcgtatcc ggaagaaatg ctgaaagaat
     4021 tcctactgat tgtcgtaata cggtattaaa atgcacattt ttactcctgt tgacttgaac
     4081 gtaatattca aatcctgttg atgagttggc actgtagttg ggtttaaata ctggtcgact
     4141 gggtgcatgt tacgattcat gtcgaaacca agaaatttgt tgggagcaat gctgatatag
     4201 ggttgcagcc agatgtaacc accatagggt gagtatcgta ggaaaaaagt gaatgtcatg
     4261 acccctgact cattctttgc cagatttccc gaccatttgt cgttgttgat atagtcggct
     4321 ttggtctttg ttttcaccga aaaaactcca acattcatac cgattcctgc tgcaaagtaa
     4381 ttgtattcct ttagcccggt gcaaataccg atattaatat caaatgttct tagccttgcc
     4441 cgtagttgtt tttccaccca aaatcccgaa tcgtacatac cagagctaac agtttgggta
     4501 gcccctgtat atccgatttc tgcaaataat ggctcgtaag cggtgccaat tgcacaagta
     4561 aaaccgtcca tataatagaa atgttccatg gtctcctgaa gattagggtg ctcccagttg
     4621 tagtaatcca ccaccagatt cagattgtcc atttttaaag gacaattggc gttataacct
     4681 gcaataacat agtactgagc gcgaagcact gaagctgata atgttaggat gattgctgtg
     4741 agcattgccg accggagaag tattgcaatg atatttttac tcatttcgac ttttattaat
     4801 ggaggtttta tccgttaaaa aaaacattta aaagtagcaa aaattatgct aatattatgt
     4861 ggtttgtgtt agcaaaataa caacgcattt aacgaaacaa gttgtatttt tgaaactcct
     4921 taataaaagc ctgtgagaaa gaagttgttt atgttatatg gggcggtttc gttgttggcg
     4981 ggatgcattc tgtggtatgt gttttgtctt cctgaacctc ttttcagcga tccggtgagt
     5041 attgtgttgc tcgataacag aaacgagatt cttggagctc aaattgccag tgaccaacaa
     5101 tggcgttttc catacaaaaa tgatgttcct gaaaaatacc ggaaatgctt gatttgtttc
     5161 gaggataaac gcttttacag ccacgcggga ttcgatccta ttgcttttgt cagggcaatg
     5221 gttcagaatg tttcgaccgg tgagaagaaa agtggcggca gcactattac catgcaggta
     5281 atcaggttgc agcgcaaagg caaagttagg agtgttggag agaagatcat cgagactatc
     5341 atggcaacgc ggttggagct gggttactca aaagatgaaa tacttgcact gtatgcatcg
     5401 aatgcaccgt ttggcgggaa tattgttggg ctcgatgccg cttcgtggag atatttcggg
     5461 agaacccccg acaaactttc gtgggcagag tcggctatgc ttgctgtact gcccaacagc
     5521 ccttcactca tccacacggg taaaaaccga gaattactca agctcaaacg caaccgtttg
     5581 ctgaaaaaac tatatctgaa ccacgaaatc gatagcctta cctatgtgct tgcactcgat
     5641 gaggatatcc ctgctcaccc gtatccatac ccaatgcatg cacggcacct tgttgggaga
     5701 gcaaattccg aaatacccaa ttcaagaagg cttggcccga tgattacaac tattaataaa
     5761 gaaattcagg aaaaggtact tgcaattgct acgacccatc aaagaagcct ggctcataac
     5821 gaaa
//
LOCUS       DAOOQP010000152         5735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_16989_length_5735_cov_5.582218, whole genome shotgun sequence.
ACCESSION   DAOOQP010000152 DAOOQP010000000
VERSION     DAOOQP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16989_length_5735_cov_5.582218"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLK75_11820"
     CDS             complement(<1..318)
                     /locus_tag="PLK75_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39975.1"
                     /translation="MKQNLVVKISLIANLVLLIGLAVLYVLFFTENSEPKNSKNKTKP
                     KTAKNDTAQAPDLPIAYVNLDTLLLEYQMSIDLNEDLLTEHAKSKANLERQVKQFENE
                     YNAF"
     gene            444..2501
                     /locus_tag="PLK75_11825"
     CDS             444..2501
                     /locus_tag="PLK75_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOY39976.1"
                     /translation="MINEKLIKPESIVVVGGSDDTSKPGGKLLENLKDGTFKGKLYVV
                     NPKSDIVQGIKAYPGVDDLPQTDMAILAIAAGYCLQTVETLINKKGTRAFIILSAGFS
                     EESKAGAEIERKIVNAINAVGGTLIGPNCIGVFNTNYSGAFTLPVPAYNNQGVDFISG
                     SGATAVFILETAISRGLRFSSVYSVGNSAQTGVEEILEYMDETFDPILSSKIKLLYIE
                     NVGKPEKLLKHASSLIRKGCRIAAVKSGGSEAGSRAASSHTGALASSDLAVESLLKKA
                     GIVRCNGREELANVAGLFTLPKPKGKRIAIITHAGGPAVMLTDALSNGGLEVPHIEGE
                     AAKNLLSKLFPGSSVNNPIDFLATGTAEQLGNIIDACENDFSNIDAMAVIFGSPGLFK
                     VYEVYDVLYEKMKSCKKPIFPVLPSIINVKDEIAHFIAKGNVFFPDEVNFGIALSKML
                     NTPDPEPAIQALPEIDVAAIRKVIENASDGYLTPDETNKLLDAAGIDRSNARVIYTEE
                     EAVAFAAETGFPQVMKVVGPVHKSDVGGVVLNVSDEATVRNEFRRLMKIKDTYAVQMN
                     LMLFGAEVFIGAKREEKFGHMVLCGLGGIFIEILKDVATNLAPVSIEKAHQMIRSLKS
                     YGIIKGARGQEPVNEAILAEQVSRVSALVLAAPEIFEMDINPLLGNNKYVKAVDARIR
                     IEK"
     gene            complement(2547..3137)
                     /locus_tag="PLK75_11830"
     CDS             complement(2547..3137)
                     /locus_tag="PLK75_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HOY39977.1"
                     /translation="MKSGFFNSNSYFINEKVNFLKFENEYKIFSEEGKEVGAVKQKLT
                     GSEKMQRVIFDKANLPFRLDIVDNEGAIQASLVRGWSFLLSKITVLDGNGIKIGSIKQ
                     SFSLLKPHLVIFDESNCEVAEIEGDWIGWSFEITDPDGAVLGAINKQWSGLAKELFTT
                     ADNYNVTITQQVNSANRRALIVTCAITIDMILKENQ"
     gene            3319..4671
                     /locus_tag="PLK75_11835"
     CDS             3319..4671
                     /locus_tag="PLK75_11835"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOY39978.1"
                     /translation="MLNNTILLDFTGALFGALFPLFFFAVPVIIVIVIVRMVKKVNQQ
                     QKTDIKDSFQSVFAAFNNQTKNYCGENLKAAKSGNKYGFVDNSGWLVIDYQYDSAYSF
                     TEGLAAVCRNSKWGYINSLNEYVIVDEFENAKPFSEGLAAVCLKGKWGFIDKTVEFVI
                     DVQFDDAGSFSEGLADVMKGNVWYKIDSNGVLFTPNSTADTTSPVIKPEFNENKISDD
                     IPNVLDLLNDIKTNENIDSTVSAGDSPYNHDMVKQQPYGVLKPIMKDFKYGFTDSEGN
                     IVIEPIYDEVSDFSEGYARVKMHWKYGFINEQGQETVPCTYDDASGFMNGMARVKLNF
                     KTGFINTLGELVVPIEFDDVGEFIDGVCIVTKNWKKGLINYQGQLLLNPEYDEIGKFI
                     DGLSRVKKAWKYGLINIDGVEVAACIYDELSEFNNGKAAFRKEGVSGYIGKTGSVLSD
                     "
     gene            complement(4668..5480)
                     /locus_tag="PLK75_11840"
     CDS             complement(4668..5480)
                     /locus_tag="PLK75_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39979.1"
                     /translation="MKSINVSFIFLFLYSFIFAQNTEFKVYFSAGNVYLKNKGTSSYI
                     TSDTPLAENSVICIPRDAYVVLINKAEMPMVISEEGEYSMRKITNLYSKLKESNLTDE
                     FFAYIAGQMFKSEDKKYVGGGVYKLVEPDMEGFSKKPDPGSCIMDTVINFEWFYYVGE
                     TTYLKIVDAKNDSLVCVISTSEYSVSLNISTCNMIRGKTYLWSVEDTPGLVKEKESLS
                     VLKLAGPDDISDFNARLLEIENSVSNPKMRKLAKIRLYVDNNVYPMPDYANF"
     gene            complement(5550..>5735)
                     /locus_tag="PLK75_11845"
     CDS             complement(5550..>5735)
                     /locus_tag="PLK75_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39980.1"
                     /translation="AVKHKELLYDLKLAGDEDIFEFSSRLNEIENSTSNEEMIKLAKI
                     RLYIDYKVYPMPNYDDL"
BASE COUNT         1804 a         1060 c         1138 g         1733 t
ORIGIN      
        1 gaatgcatta tattcatttt caaattgctt cacctggcgt tccagattgg ccttcgactt
       61 ggcatgttca gtaagcaaat cttcgttcaa atcaatcgac atctgatatt caagcagcaa
      121 agtatcgagg tttacataag ctatcggtaa atcaggggcc tgtgcagtat cgtttttagc
      181 agttttaggc ttggttttgt tttttgaatt tttaggctca ctgttttcag taaaaaacag
      241 cacatacaac actgcaagtc caataagaag aaccagattc gcaataaggc tgattttaac
      301 aacaaggttt tgtttcatgt tttaataatt aagagtatga caaaattaaa aatattttac
      361 tcacttttta tggaattaac gaaacataag gttttttgtt accaaatgtt tttttaagtt
      421 tgttagctag aaaatcttat atcatgataa acgaaaaact aataaaacca gaaagcattg
      481 ttgtagtggg tggcagcgac gacacaagca aacccggtgg taaactcctc gaaaacctaa
      541 aggatggcac ttttaaaggg aagctgtatg tagttaaccc aaagtctgat attgttcagg
      601 gcattaaggc ttatccgggt gttgacgatt tacctcagac cgatatggca attctcgcta
      661 ttgcagctgg gtactgtctg caaaccgttg aaaccttaat aaataagaaa ggcacacgtg
      721 cttttattat tttgtcggca ggatttagtg aggaaagcaa agcaggcgca gaaatcgaaa
      781 gaaaaattgt aaatgctatc aatgccgttg ggggtacgtt aataggaccc aactgcatag
      841 gggtcttcaa tactaactat agcggtgctt tcacactgcc ggtgccagct tacaacaatc
      901 agggtgttga ttttatctcc gggtcaggag ccacagcagt atttattctt gaaacagcca
      961 tttcaagggg gctcagattc agcagtgtat actcagtagg caatagtgcc cagaccggtg
     1021 ttgaagaaat tcttgaatac atggatgaaa ccttcgaccc gatattaagc agtaaaatca
     1081 aattactata tatcgaaaat gttggaaaac ctgagaaact gttaaaacat gcctcatcgc
     1141 ttattcggaa aggctgcaga attgctgctg taaaatcagg aggttctgaa gccggtagcc
     1201 gtgcagcatc atcgcatacg ggagctttgg caagcagtga ccttgccgtg gaatcgctgc
     1261 taaaaaaagc aggcattgtg cgctgcaatg gcagagaaga gctggctaat gttgcaggct
     1321 tgtttactct tcctaaaccc aaagggaaac gcattgcaat aataacccat gcaggcggac
     1381 cagcagttat gctaacagat gccctcagca acggaggatt agaagtccct cacatcgaag
     1441 gcgaagccgc caagaatcta ctctcaaagc ttttcccggg ttcatcggta aacaatccta
     1501 tcgatttttt ggctacaggt actgccgaac aacttggaaa cattattgat gcctgcgaaa
     1561 atgatttcag caacatcgat gcaatggcag ttatttttgg aagccccggg ctttttaaag
     1621 tatatgaagt gtatgatgtt ctttatgaga aaatgaaatc atgcaaaaaa cccatctttc
     1681 ctgtattgcc atctatcatt aatgttaaag acgaaatcgc acattttatt gcaaaaggca
     1741 atgttttctt tccggatgag gttaattttg ggattgcttt gtcgaaaatg ctgaacacac
     1801 ctgaccctga acctgccatt caggctttac ctgaaattga tgtcgctgca atcagaaaag
     1861 ttattgagaa cgcatctgac ggatatctga cgccagatga aacaaacaaa ttacttgatg
     1921 ccgctggtat cgaccgctcc aatgcgcgag ttatttacac tgaagaggaa gctgtagcat
     1981 ttgctgcaga aaccggtttt ccacaggtta tgaaagtagt tggtcctgtg cacaaatcag
     2041 atgttggtgg cgttgttctg aatgtttcgg atgaagccac agtacgcaat gaattcagaa
     2101 gactcatgaa aattaaagac acctatgcag tgcagatgaa tttaatgctt tttggggctg
     2161 aagtatttat tggtgcaaaa agggaagaaa aatttggtca catggtgctc tgtggtctgg
     2221 gtgggatatt tattgaaatt ctaaaagatg ttgccacaaa ccttgctcct gtttccattg
     2281 agaaagcaca tcaaatgatc cggtctctga aatcgtatgg aataataaaa ggcgcccgtg
     2341 gacaggagcc tgtgaacgaa gctattttag ccgaacaggt ttcccgcgtt tcggctctgg
     2401 tacttgctgc cccagaaata tttgaaatgg atataaaccc gttgctgggc aataacaaat
     2461 atgttaaagc ggtggatgcc cgaatcagga tagaaaaata aaattgctca aacctctttc
     2521 aaaagctgtt acaagaacag cttttattat tgattttctt ttagaatcat gtcgattgta
     2581 atcgcacagg ttacaataag agccctccga tttgctgaat tcacttgctg agtaatggtt
     2641 acattgtaat tgtcggctgt tgtgaacaac tccttggcaa gccccgacca ctgtttgttg
     2701 attgccccca acacagctcc atcgggatcg gtaatttcga aagaccaacc aatccagtcg
     2761 ccttcaattt cagcaacctc gcaattgctt tcatcaaaaa taaccaaatg cggtttcagt
     2821 agcgaaaagc tctgttttat actgccaatt ttaattccat ttccatcgag aacagtaatc
     2881 tttgacaaca gaaacgacca gccacgaacc agacttgcct gaatagcacc ttcattatca
     2941 acaatatcga gtctgaaagg caggtttgcc ttatcgaaaa taacacgttg cattttctca
     3001 ctgcctgtta atttttgctt tacagcaccg acttccttac cttcttcgct gaaaattttg
     3061 tattcatttt cgaacttcag aaaattaact ttttcgttta taaagtaact gttcgaattg
     3121 aaaaatccgg atttcataca agtgggtcaa tagggttata aataattaga atcaacacaa
     3181 aattataact tattataata ttaattcgtt gttttaattt cattttttct attacacaac
     3241 attgtttctt ttctgccaga aataactata tctttgttat tgatttgcat ttattcggta
     3301 aaaaaaatct caaatcaaat gctaaataat acaatactgc ttgattttac cggtgcattg
     3361 ttcggagcgc tcttccctct ttttttcttt gcagtaccag taatcatagt tatcgtaatt
     3421 gtaagaatgg taaaaaaggt aaatcagcaa caaaaaaccg atattaagga ttcatttcag
     3481 tcggtttttg cagcattcaa caaccaaacg aaaaactatt gcggggaaaa cctcaaagca
     3541 gcaaaatcag gaaacaagta tggttttgtc gataattcag gatggcttgt tattgattac
     3601 caatacgata gtgcctatag ttttacagaa ggactggctg ctgtctgtcg taattcgaaa
     3661 tggggttata tcaacagttt aaatgaatac gtaattgttg atgaattcga gaacgcaaaa
     3721 ccatttagcg aagggcttgc agccgtttgt ttgaaaggca aatggggttt tatagataag
     3781 acagttgaat ttgtaattga tgttcaattt gatgatgccg ggagtttttc ggaaggtctg
     3841 gcagatgtga tgaaaggaaa tgtgtggtat aaaattgata gcaatggtgt tcttttcacc
     3901 ccaaacagca ctgcagatac cacttcaccc gtgataaagc cggaattcaa tgaaaataaa
     3961 atctcagacg atattcccaa tgtactggat ttgcttaatg atataaaaac aaacgaaaat
     4021 attgacagca cggtgtcagc aggggattcg ccgtacaacc atgatatggt aaaacagcaa
     4081 ccttacggtg tgctcaaacc cataatgaaa gattttaaat acggttttac cgatagcgaa
     4141 gggaatatag taattgaacc gatttatgac gaagtgtctg atttctccga aggatatgca
     4201 cgtgtaaaaa tgcactggaa atatggattt atcaatgaac agggacaaga aaccgtgccg
     4261 tgtacctacg atgacgcatc aggctttatg aacggaatgg cgcgcgtaaa gcttaacttt
     4321 aaaaccggtt ttatcaacac cttgggtgag ctagttgttc cgatagaatt cgatgatgtg
     4381 ggtgagttta ttgatggagt ttgcattgtt acaaaaaatt ggaaaaaagg gcttatcaac
     4441 tatcagggtc agcttttatt aaaccctgaa tacgatgaaa taggtaaatt tatcgacggg
     4501 ttatcgcgtg taaaaaaggc ctggaaatat gggttaatca atattgatgg agttgaagtt
     4561 gctgcctgca tttacgacga attaagtgaa tttaacaatg ggaaggctgc ttttcgcaaa
     4621 gaaggggttt caggctatat cggtaaaaca ggttctgtgt tatctgatta gaaattggca
     4681 taatcgggca tcgggtaaac gttgttatca acgtagagtc tgattttagc aagttttcgc
     4741 atttttgggt ttgatactga attttcaatt tcgagcaaac gtgcattgaa atcagaaata
     4801 tcatcaggac cagcaagttt aagcactgaa agcgattctt tttccttcac caaacccggg
     4861 gtatcttcca ccgaccaaag gtaggtttta ccacgaatca tattacatgt actaatattc
     4921 aggcttacac tatattcact cgtagagata acgcaaacta acgaatcgtt ttttgcgtca
     4981 acaattttaa gatacgtggt ttcacctacg taataaaacc attcaaaatt aattaccgta
     5041 tccataatac atgatcctgg atccggtttc ttactaaaac cttccatatc aggttctaca
     5101 agcttatata caccccctcc cacgtacttt ttgtcttcac ttttaaacat ctgtccggca
     5161 atatatgcaa agaattcatc tgtgaggtta ctctccttga gttttgaata cagatttgtt
     5221 atttttcgca tgctatactc cccttcttca ctgataacca taggcatttc tgccttattt
     5281 attaatacaa catatgcatc tctcgggatg cagattacgc tgttttcggc aagaggagta
     5341 tcgctggtta tgtatgatga ggttcctttg tttttaaggt aaacattccc ggcagaaaaa
     5401 tacaccttaa attctgtgtt ctgagcaaat ataaaactgt acaaaaaaag aaaaataaaa
     5461 ctgacattaa ttgatttcat cttcgtaaaa attaattaat catataaatt attaataata
     5521 atatattttg catcatataa attaaaaatt tacaagtcgt catagttagg cataggatac
     5581 actttataat caatataaag ccgtatttta gccagtttaa tcatctcttc attagatgta
     5641 gagttttcaa tttcatttaa gcgagaagaa aattcaaaaa tatcttcatc tcctgcaagt
     5701 tttagatcgt aaagtaattc tttatgctta acggc
//
LOCUS       DAOOQP010000153         5651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_17286_length_5651_cov_4.837205, whole genome shotgun sequence.
ACCESSION   DAOOQP010000153 DAOOQP010000000
VERSION     DAOOQP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17286_length_5651_cov_4.837205"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLK75_11850"
     CDS             complement(<1..227)
                     /locus_tag="PLK75_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY39981.1"
                     /translation="MSDLKYTIPAIFNQSVEKNWNRPAFSFVSETPYTYREVQVLVKS
                     LMSFLNRLGIKKSTKVAILSDNSPNWAMSYLA"
     gene            452..1747
                     /locus_tag="PLK75_11855"
     CDS             452..1747
                     /locus_tag="PLK75_11855"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY39982.1"
                     /translation="MRQKTIVLFYPQNLLSHYLRCISIAKQLGEDYDVFFVGSKNYNR
                     FVEKEHFLIIENNSIAIDEVVQKAMSFDFSWINKGNLEKAFLNDLEIIERCKPDIIVG
                     DANLSLRMVAEKMHVFYVAVENNYLSNRYSDIRPVPHTHKAWNFRDKMSDANWENLIS
                     FVEKIVLRSVHKPFRYLRKKYGLSQQRNLLDEFEGDVVLLLDNPDIFPLGALESNKFC
                     IGPQLYRNEGSEAEIKTWIQERKHRKTIYVSMGSSADVEKNTYFMKHLSTSEFNVIFS
                     GNGNCVTDTVFSKQFINFTEIAEYIDVYVCHGGNGSIYQALFHAIPLLMIPSIFEQEW
                     NVHRIVKLNLGSVHYPEKGITDFLKKLNIQISRKDSTATSAMKNKLLEGKQYHDVSEI
                     FEQIRRQLFPADYPQTGIGNNSVQDKKLISENITTEPLC"
     gene            complement(1649..3124)
                     /locus_tag="PLK75_11860"
     CDS             complement(1649..3124)
                     /locus_tag="PLK75_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOY39983.1"
                     /translation="MSTWLVASILILYFGALVLIGFITSRKATSSTFFTGNRQSPWYL
                     VAFGMIGASLSGVTFISVPGMVINESMAYFQVILGYIIGYIVIAKVLMPVYYKLNLIS
                     IYEFLGQRFGLWSHKTGAAFFILSRTLGSAMRLYLATLVLQLFLFDGLGVPYWVTAVA
                     TVVLIWIYTFKGGIRTIVFTDTFQTFFLVSAVVISVVIISKTLGLNLFELFGKITDSK
                     NIHGESLSKIFFFENYKSALFFPKQFFGGAFLAITMTGLDQDLMQKNLTCRNIKDAQK
                     NMYSFTAVLVVVTFLFLLLGGALYVYAGANGLALPTDANGSIISDRVFPTLAMGEFGT
                     LAGIFFLLGITASSYASADSALAALTTGFCIDFLNFRKRTEKQKQLIKLFVHIGFSLL
                     FLAIILIMKEVASKDLISIVLTVASYTYGPLLGLFFFGIITRRKITDKLTPLVCIVAP
                     MLCYILSSNSAQWFGGYVFGYELLILNGIITYTGLWIISRK"
     gene            3293..3922
                     /gene="recR"
                     /locus_tag="PLK75_11865"
     CDS             3293..3922
                     /gene="recR"
                     /locus_tag="PLK75_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOY39984.1"
                     /translation="MENNFISSKLFADAVSELSKLPGIGQKTAVRLVLHLIRQENSEV
                     EALGNAIITMKKEIRFCKQCHNISDSELCHICGDNRRDSSTICVVAGIQDVVAIENTQ
                     QYRGVFHVLGGVISPLDGIGPQHLHIPELIDRARNGITREVILALPPTMEGDTTGFYI
                     FRKLENSQIPVTTIAKGVSVGDELQYADEITLGQSILNRRPFLTTKISY"
     gene            complement(3930..5240)
                     /locus_tag="PLK75_11870"
     CDS             complement(3930..5240)
                     /locus_tag="PLK75_11870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOY39985.1"
                     /translation="MKVIMVGTGYVGLVSGTCFAEMGVNTVCVDIDKTKIDNLKKGII
                     PIWEPGLEDMVKRNTECGRLSFSSSLKDSIAGADACFIAVGTPPDEDGSADLQYVIAV
                     ASEIGQYMNDYLVVVTKSTVPIGTSNKVKEAIKNELKKRKEKIKFDVASNPEFLKEGD
                     AIEDCMHPDRIVIGTESERAREILNRLYKPFVLNGHPIIHMDIPSAEITKYAANAMLA
                     TKISFMNDMANFCELVGADISAVRRGIGSDRRIGQYFIYAGIGYGGSCFPKDVKAIIK
                     SADKLNYSLEILKAVENVNDRQKSRLFVKAKQYFSGDLSGKKVVLWGLSFKPNTDDMR
                     EAPSLVLIENLLSAGCKVFAYDPVAMQETKRRIGKIITYAENPYEALAGADALFLVTE
                     WTEFRVLNFDKMQLMKHKVVFDGRNIYDPNELIAHGFSYFGIGR"
BASE COUNT         1810 a         1112 c         1069 g         1660 t
ORIGIN      
        1 gccaggtacg acattgccca gtttggagaa ttatcgctca gtatggcaac ttttgtggat
       61 tttttgattc ccagtcggtt taaaaatgac ataaggcttt tcaccaaaac ctgaacctcc
      121 cggtatgtgt acggtgtttc agatacaaat gaaaaagcgg gtctattcca gttcttttca
      181 accgattgat tgaaaatcgc tggtattgta tattttaagt cactcatttc taataaattc
      241 gtcgacaaaa gaacgaaata aaacaatgat tattctggat atcataaaaa gttattaata
      301 tttacctgaa ttttatacca acatttcatt ttataggtta ttatatttga tggcaataca
      361 aatacggtaa ttaatcgctt atttattaat taatttttta acaaacctgt ttaaaaactc
      421 ctcatttact acttttacaa aaaacaaatc aatgcgacaa aaaaccattg tcctgtttta
      481 tccacaaaat ctattatcgc attatttgcg gtgcatatcc attgcaaaac aactgggaga
      541 ggattatgat gttttttttg ttggatcaaa aaactataac cgctttgttg aaaaagaaca
      601 tttcctaatt atcgaaaaca acagcattgc catagatgaa gtggttcaga aagcaatgtc
      661 ctttgatttt tcatggataa acaaaggaaa cctcgaaaag gcgtttctta acgatttgga
      721 aattatagaa agatgcaagc ccgatattat tgttggtgat gcaaatctta gcctgcgaat
      781 ggtagcagaa aaaatgcatg ttttttatgt tgccgtagaa aataattatc tgagtaaccg
      841 ctactccgac attcgcccgg ttccgcatac ccataaagca tggaatttca gagataaaat
      901 gagtgatgcc aattgggaaa acctgatcag ctttgttgag aaaattgtac ttaggtcggt
      961 acacaaacct ttcaggtacc tgcgaaaaaa atacggtctc tcgcagcagc gtaatcttct
     1021 ggatgaattt gaaggtgatg ttgtactatt gcttgataat ccagatattt tcccgcttgg
     1081 agctcttgaa agtaataaat tttgcatcgg accccaatta taccgtaatg aaggttctga
     1141 ggctgaaatt aaaacgtgga tacaggaaag gaaacatcgc aaaacaattt atgttagtat
     1201 gggaagcagt gctgatgtgg aaaaaaacac atattttatg aaacatcttt ctacttcaga
     1261 atttaatgta atcttttcgg ggaatggaaa ttgtgttact gatacagttt ttagcaaaca
     1321 atttatcaat tttaccgaaa ttgctgaata tatcgatgta tatgtgtgtc atggaggaaa
     1381 tggaagtatt tatcaggctc tgttccatgc cataccattg cttatgatcc cctcaatttt
     1441 cgagcaggaa tggaacgttc atcgcatcgt taagctcaac ctgggttcag ttcattatcc
     1501 agaaaagggg attaccgatt ttttaaaaaa actgaacatt cagatatcac ggaaagattc
     1561 aactgccact tccgctatga aaaataagct tctggaagga aagcaatatc atgatgtctc
     1621 agaaatattt gaacaaattc gcagacaact atttcctgct gattatccac aaaccggtat
     1681 aggtaataat tccgttcagg ataagaagct catatccgaa aacataacca ccgaaccatt
     1741 gtgctgagtt tgatgacagt atatagcaca gcatgggagc aacaatacaa actaaaggcg
     1801 taagtttatc agttattttc cttcgtgtaa taataccaaa aaagaaaagc ccaaggagtg
     1861 gcccgtaagt ataggaagca acggtcagca caattgatat aaggtctttt gaggcgactt
     1921 ctttcattat taatattatc gcaagaaata acaacgaaaa tccaatatgc acaaatagtt
     1981 ttataagctg cttctgcttt tcggttcgtt tccggaaatt cagaaagtca atacaaaaac
     2041 cggtggttaa tgctgcaaga gcactatcag cgcttgcata cgatgaagca gtgatgccaa
     2101 gaaggaaaaa aataccggca agggtgccaa attcacccat tgcaagcgta gggaaaacac
     2161 ggtcgctgat gattgaccca ttcgcatctg ttgggagagc cagcccgttc gctcctgcat
     2221 atacatataa tgcccctccg agcaagagaa aaagaaatgt tacaaccacc agtactgcag
     2281 taaaagagta catgtttttt tgagcgtctt taatgtttcg gcaggtaagg tttttctgca
     2341 taagatcctg atccagcccg gtcatggtaa tcgcaagaaa tgcacctcca aagaactgtt
     2401 taggaaaaaa caaagcagat ttgtagtttt cgaaaaagaa gattttacta aggctttctc
     2461 catgtatgtt tttactatca gtaattttcc caaatagttc aaacaggttc agacccaatg
     2521 tttttgaaat tattacgacg ctgattacca ctgcggatac taaaaaaaac gtttgaaaag
     2581 tatcggtgaa cacaatagtt cttattccgc ctttaaacgt gtaaatccat attaacacaa
     2641 ctgtagctac agcagtaacc caataaggga ctccaaggcc atcgaataaa aacaactgaa
     2701 gaaccagcgt tgcaagatat agcctcatgg cagagcccag tgttcgggat agtatgaaga
     2761 aagctgcccc ggttttatgg ctccaaagtc caaaacgctg cccgagaaat tcataaatcg
     2821 aaattaaatt cagtttgtag tacaccggca taagtacttt ggcaatcact atgtaaccga
     2881 taatataacc aagtattacc tgaaagtatg ccatgctttc gtttattacc attccgggta
     2941 ccgatataaa cgtaaccccc gacagagaag ctccaatcat tccgaatgca accaaatacc
     3001 atggactctg gcgattaccg gtaaaaaatg ttgaactcgt ggcttttcgc gatgtgataa
     3061 acccaatcag caccaatgca ccgaaataaa gaataagtat acttgctacc agccaggtcg
     3121 acatgatatg ttaattaatc aatgctacaa ataaccatgg atttttacga aacacaagga
     3181 ataaatacaa aagttttaaa actgcctgtg ttaacattta atcgaaaaat aaaacataat
     3241 gattgtattt tattttttgc acttaaacac aatgtgtaat tttgcagaag ttatggaaaa
     3301 caattttatc tccagtaaac tgtttgctga tgctgtcagc gaattgtcga agctgccggg
     3361 tattgggcag aaaactgcgg ttcgtttggt attgcacttg atacggcagg aaaacagcga
     3421 agtagaggct ttaggcaatg caatcattac catgaagaaa gaaataaggt tttgcaagca
     3481 atgccataat atttcggata gtgaattgtg tcatatttgt ggtgataata ggcgcgattc
     3541 atcaacaata tgtgttgtgg caggaattca ggatgttgtt gctattgaga acacgcagca
     3601 gtacaggggg gtgtttcatg tattgggagg agtgatttca ccacttgatg ggattggccc
     3661 ccaacatttg catattcccg aacttattga ccgagctcgg aatggtatta ctagagaagt
     3721 aatactggca ctaccgccaa ctatggaagg cgatacaaca ggtttttata ttttccgtaa
     3781 acttgaaaac tctcagattc ctgtaacaac cattgcaaaa ggtgtttcgg taggcgatga
     3841 attacaatat gccgacgaga ttacattggg acagtcaatc ctgaaccgac ggcctttcct
     3901 caccacgaag attagttatt aataatggct tatctgccaa ttccaaagta gctgaacccg
     3961 tgcgcaataa gttcatttgg gtcgtaaata tttcttccgt cgaaaacaac cttgtgcttc
     4021 ataagttgca ttttatcgaa attcagcacc ctgaattcag tccactctgt gaccagaaac
     4081 aaagcatcgg caccggcgag agcttcgtaa gggttttcgg cataggtgat tattttaccg
     4141 atgcgccgtt tagtttcttg catggcaaca ggatcgtatg caaaaacctt gcagccggct
     4201 gaaagaaggt tctcgataag taccaacgaa ggggcttctc tcatatcatc ggtgttcggt
     4261 ttaaacgaca aaccccagag aacaaccttt ttgccagata aatcacccga gaaatattgt
     4321 tttgctttta caaacaaacg tgacttctgt cgatcgttga cattctcaac tgcttttaat
     4381 atctcaaggg aataattcag cttgtcagcc gattttataa tagctttaac atccttcgga
     4441 aagcatgacc ccccatagcc aatgcccgca taaataaaat attgcccaat tcttctgtca
     4501 ctgccaatcc cccgcctcac agcactgata tcggcaccca caagttcgca aaaattagcc
     4561 atatcgttca taaaactgat ttttgtggca agcatagcat ttgcagcgta tttggtaatc
     4621 tctgccgaag gtatatccat gtggataata ggatgcccgt tcagaacaaa tggtttataa
     4681 agtcggttta aaatttcgcg ggctctttcc gattcggtcc cgattacaat tcggtcggga
     4741 tgcatacaat cttcgatggc gtcaccttct ttaagaaatt cagggtttga agctacatcg
     4801 aatttgattt tttctttgcg cttctttagc tcatttttaa tagcttcttt tactttattt
     4861 gaagtaccta tgggtacggt gctctttgtt actaccacaa gataatcgtt catgtattgc
     4921 ccgatttccg aagccacagc tattacatat tgcaaatcag cactaccatc ttcatcggga
     4981 ggggtgccta cagcaataaa gcaagcatcg gcgcctgcaa tactatcttt caggcttgac
     5041 gaaaaactga gtcttccaca ttctgtattt cgctttacca tgtcctcgag cccaggttcc
     5101 caaataggta ttatcccttt ttttagattg tcgattttag tcttgtcgat atctacgcaa
     5161 accgtattga cacccatttc ggcaaaacat gttcccgaaa ccaacccaac ataaccagta
     5221 cctaccatta taactttcat atgcagtcgg ctttaattcg ccgcaatatt aataaatcaa
     5281 atccgataaa ttgtttttat aaatcataat attacaaaat catctgtaat aattgggtaa
     5341 ggaaatttgt ttcacttacg aatattccct gccggaagca actatattcc ttattagtgc
     5401 cattttgtcg atgaaaatgt ttttttgagt aaccatttag ggtaatttat atgtatattt
     5461 gtactaataa ccctcataaa ccgtcagaat gaagaatatt gaatttgata acgatctggt
     5521 gctgatgcgc aatcagctat tttcgaaagc aatgtcgctg tcggtaaaca ggcaggatgc
     5581 cgaagacctt acacacgaag cgattgagaa agcaatattt aataaagcca aatttcgcaa
     5641 aaacgacagc c
//
LOCUS       DAOOQP010000154         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_17436_length_5615_cov_4.742086, whole genome shotgun sequence.
ACCESSION   DAOOQP010000154 DAOOQP010000000
VERSION     DAOOQP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17436_length_5615_cov_4.742086"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLK75_11875"
     CDS             complement(<1..320)
                     /locus_tag="PLK75_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HOY39986.1"
                     /translation="MSSNQKISIRFFDDREVRALWDEQNAKWWFSVLDIVAVLTDQND
                     YTKTRNYWKYLKAKLKKEKNELVSGTTQLKLLASDGKRYVTDMLDYNGIIALGKQFAG
                     KKANR"
     gene            776..2503
                     /locus_tag="PLK75_11880"
     CDS             776..2503
                     /locus_tag="PLK75_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39987.1"
                     /translation="MRKNLLLVALLFAAAVAGAQTTIFSENFDEFTAGQTVNSQQPLF
                     YPWTGQAAENSYVSDVQSHSETKSMKIINNNDMVFNFGDKTSGEYDVNFWIYIVSGQG
                     GYFNIEHDFGNSWAFAFYFRTDGTCDLIQGGVTTNFAYAADTWMHVELNINLETDQAT
                     AWVDETEILSWTFSNEESAAGGQNKLDVINFYGLNQTSTGVNNSEYYVDDFEYIEIQS
                     GLIPPTIDIETTEISTFGQDEELLGFSNIGEEPMNYSAYPVYEDPSISSNLINGQMNY
                     DLDIITAIGWENSFDVDVAVRMGNDLTSQHLGQTIHYISFFINDLPASETVTVYVWAK
                     GDYSQPGTSTVLFEQEMTPTAQTWNAVTLDNPIVITGEEIWIGYHFTSPAGLFTLGVD
                     DQPVVPGTCYLRTGPVWSEFPGVDGTGNFCIRAEVEGLGWPAWLLVTPGTGTVAAGQE
                     ANLTLSFNTVALTAGTYYANVVIGANDPDQQWTEVPVTLDYLVGIENQTSIGVMTYPN
                     PTRDIVNLVSDHQIESVTVFNVTGQRVISQEINSSRAAVDVSNLPTGSYIIEVSVQGN
                     ISRSKLVVE"
     gene            complement(2641..3954)
                     /locus_tag="PLK75_11885"
     CDS             complement(2641..3954)
                     /locus_tag="PLK75_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39988.1"
                     /translation="MKKLTFVLLFLALAIAGFAQEKCTFFIDNGAKQVTELLCGTFTD
                     LMVKVPLPKTAPNFDKVVVHYANSTKPNEISRVEFEGAGALKTVAGTTKNLWIHDPSN
                     TSGTQGGAHGDFNLEINDAYVNMNCLCTYPKRDRFESITITIKAVGYTVTGSEQYYSE
                     KAGAWKTKYTYDKGTLLSEGSIVIKQEEFEGFYVDKNVKIVFPDPSISFVKTKTITTN
                     SGMAPDNLYKVITCTSTTLDLVETGQKESQQKTSVVINIVKEADIENLDSYKKAKAAN
                     AAYTPLQYTKDAIECWYKYKSFGCTPADDMKKNKYNTDIHIGSIVPNGFIEELYKGMD
                     VSNELLKKKEVKNQWILWSDKTMGKNTFGYGYIEKMFMANCLTGKPEYNPSKFESVEF
                     YVYYKKPNIYIISPYYVNDQVRGQYYDEQKQKGYVKFVMENIEFQ"
     gene            complement(4143..5543)
                     /locus_tag="PLK75_11890"
     CDS             complement(4143..5543)
                     /locus_tag="PLK75_11890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY39989.1"
                     /translation="MAEKLHVSHSEKSSGTGLITVAMLAAFITPFMGASVNVALPVIS
                     REFTMNASTMSWVAMSYILASAVFLVPFGKYSDITGRKKLFIAGMIIFAIGSLLCSVS
                     QSAGMLISSRVIQGIGSSMVFSTSMAIVVAAFAPEKRGRAIGLISASVYIGLSAAPVL
                     GGLMTQFIGWRSIFVLTMMLCLIVITIANRVIVEQHQNLTREKFDLSGSLLYLISMSL
                     LMYGLSRITEAFAIIMTAIGVAGMVFFVIIEYRITSPVMNIKLIRHNRVFAFSNLAAL
                     INYAATFAVSFILSLYLQYVKGLSPGEAGLVLLAQPLVMAIVTPFAGHLSDRIQAGLL
                     ASLGMAISASGLFALIFLHHDSGFATIVVCLFVVGLGFGIFASPNTNSVMSSVEKPQY
                     GVASAFLGTMRLTGQMLSMAIATLAIYLFIGKSPIKPENIDKFMNSVQIVFIIFSLLC
                     FVGIFASLARGKEKGK"
BASE COUNT         1653 a         1137 c         1123 g         1702 t
ORIGIN      
        1 cggtttgctt ttttaccagc aaattgtttg cccaatgcaa taattccgtt gtaatcgagc
       61 atatctgtca catatcgttt gccgtcactt gctaacagtt tcagttgggt agtgccacta
      121 accaactcgt tcttttcttt ctttaatttg gcttttaaat atttccagta gttgcgagtt
      181 ttggtatagt cgttctggtc ggtaagaaca gctacaatgt ccagcaccga gaaccaccac
      241 ttggcgtttt gttcatccca caatgcccga acctcccgat catcgaaaaa acgtatggat
      301 attttttggt tactgctcat ttttcaagtt atttcgctca ttccccaata gtgctgccca
      361 aagtattcat tgacaaattt aataagtctt acgcaattaa cgtgctaatg aatgtattaa
      421 atcataatct ggtaacaacc tacccatctg ctgtgtaaat tctacctcat ccaagtcacc
      481 cgcagaggtc tcggatgtac cgatattatc gaatgtactt ataacaaaaa tcaaatcatc
      541 tcagagtctc gttggggtga accgtagcgg aaaagaaatt catgatctaa agcaattagg
      601 tccattacag tatttaataa acttggtgtt tttattattt tgtttaacac ttctttttca
      661 gatctaatcg agttattgta actttcgaag ttcaattttt aaaatttata aaatttattt
      721 cttcattata tcaaattttt gtatttttac ataaatctaa ttaaacaaca caattatgag
      781 aaaaaattta cttctggtgg cactattgtt tgcagccgct gtggctggtg cacaaaccac
      841 cattttcagc gaaaacttcg acgaatttac cgccggacaa acagtgaatt cccagcaacc
      901 cttgttttat ccgtggactg gtcaggcagc tgagaacagt tatgtaagtg atgttcaatc
      961 gcattcggag accaaatcca tgaaaatcat caacaacaac gacatggttt tcaattttgg
     1021 cgataaaacc agcggtgaat atgatgtaaa tttctggatt tacattgtgt cgggtcaggg
     1081 aggatatttt aatattgaac acgattttgg caattcatgg gcgtttgctt tctatttcag
     1141 gacagatggt acctgcgacc tgattcaggg tggtgtaaca accaattttg cttatgctgc
     1201 tgatacctgg atgcatgttg aactgaatat taatcttgaa accgatcagg caacagcctg
     1261 ggttgatgaa accgaaatcc tttcgtggac gttttcaaac gaagaaagtg ctgcaggagg
     1321 tcagaataaa ctggatgtta ttaatttcta tggattgaat caaacgagta ctggtgtaaa
     1381 caattcggag tattatgtcg atgattttga atatattgaa attcaaagcg gtttgattcc
     1441 tccaactatc gatatagaaa ctactgaaat atctactttc ggacaggacg aagaattgct
     1501 tggtttctca aatatcggag aagagcctat gaattacagt gcatatccgg tttatgaaga
     1561 cccttcaata agctcaaatt tgattaatgg tcaaatgaac tacgaccttg acattatcac
     1621 agctattgga tgggagaata gtttcgatgt agatgttgct gtgcgaatgg gcaacgattt
     1681 aacatcgcag catttaggcc agacaataca ctacattagt ttctttatta acgatttgcc
     1741 ggcttccgaa actgtcacgg tatatgtttg ggcaaaaggt gattatagtc agcccggaac
     1801 gtcgacagtt ttgtttgaac aggaaatgac tccgacagcc cagacatgga atgctgttac
     1861 cttagataac ccgattgtaa ttacaggcga agaaatatgg attgggtacc attttactag
     1921 tcctgcaggt ctttttaccc ttggggtaga cgatcaaccg gtagttccgg gaacctgcta
     1981 tttacgtaca ggccccgtat ggtctgaatt ccccggtgtt gatggtaccg gtaacttctg
     2041 tatccgtgct gaagttgagg gattgggatg gcctgcatgg ttattggtaa ctcctggcac
     2101 aggaactgtg gcagctgggc aagaagcaaa cctgactctg agttttaata cagttgctct
     2161 cactgctggg acatactacg caaatgtagt tatcggtgca aacgatccag atcagcagtg
     2221 gacagaagtg cctgttactc ttgattatct tgtcggtatc gaaaatcaga cctcgattgg
     2281 agtaatgact tatccaaacc caacccgtga tattgtaaac ctggtttccg accatcaaat
     2341 tgaatcagta actgtgttta atgtaaccgg gcaaagggtt atttcgcaag agataaactc
     2401 cagtcgtgct gctgttgacg tgagcaacct cccaacaggt agctacatta ttgaagttag
     2461 tgttcaagga aatatctcaa ggagcaaact cgtggttgaa tagataagtg tagcattaag
     2521 taaagaggct gcctcataag ggcagcctct tttatgatta tgaaactcaa tcaggttgtc
     2581 aatgtaacag cttaatattc atactgcaaa tgtcatatgg ccttcagcaa caattccaac
     2641 ctattgaaat tcgatattct ccataacaaa ttttacatag cccttttgct tttgttcatc
     2701 gtaatactgc ccacgaactt gatcgtttac ataataggga gatataatat aaatgttggg
     2761 ttttttataa tacacataaa actcaaccga ttcaaacttc gaaggattat attccggctt
     2821 gccggtaagg caattcgcca tgaacatttt ctcgatgtaa ccgtagccaa aagtgttttt
     2881 ccccatggtt ttgtccgacc acaatatcca ctgattctta acttctttct tttttaatag
     2941 ctcattcgaa acatccatgc ccttgtacag ctcttctata aacccgtttg gaactatcga
     3001 acctatgtga atatcggtgt tatatttgtt tttcttcata tcatcagcag gtgtgcatcc
     3061 gaacgatttg tacttatacc agcattcgat cgcatcttta gtgtattgca ggggagtgta
     3121 ggcagcgttg gctgctttgg ctttcttata cgaatcgagg ttctcgatat cggcttcttt
     3181 aacaatattg attactacgg aagttttctg ttgactctct ttttgtccgg tctcaaccaa
     3241 atcgagtgtg gttgatgtac aggttattac tttgtacagg ttatcggggg ccattccgga
     3301 attggtggta atggtctttg ttttaacaaa agaaatactg ggatccggaa atacgatttt
     3361 tacattttta tcaacataaa aaccctcgaa ttcttcctgt tttattacaa tacttccttc
     3421 cgaaagtaag gttcctttat cgtaggtgta tttggttttc caggcaccgg ctttctcgga
     3481 gtaatattgt tcagaaccgg taactgtgta tcccactgct ttaattgtga tagtgatcga
     3541 ttcaaatcta tcccttttgg ggtaagtaca cagacaattc atgttgacat aagcatcgtt
     3601 aatttccaga ttaaaatcac cgtgagcacc accctgggtc ccggaggtgt tggacggatc
     3661 atgaatccat agatttttag tggtgccggc aactgttttc agtgcccctg ctccttcgaa
     3721 ctcaacacga gatatttcgt ttggttttgt tgagtttgca taatgaacaa ccactttgtc
     3781 gaaattagga gcggtttttg gcaggggcac ttttaccatc aggtcagtga aggtgccgca
     3841 taagagttct gttacctgct tggctccgtt atcaatgaaa aaagtgcatt tttcctgcgc
     3901 aaaacctgca atggccagtg caagaaacaa tagaacaaaa gttagttttt tcatggcata
     3961 cgtatttagg ttaggactcg gttagcataa caaatatagt aaatattatt taaaaaccaa
     4021 cagatatttg taatttaatt agccctgtcg tttgtttgct atcaaagcaa taagagatca
     4081 gactgaaagt gctgcttaat tgctaacaat cgacatttta cagaaattac tttgcaatcg
     4141 gtttacttac ccttttcttt acctctggcc agtgatgcaa atatgcccac aaagcatagc
     4201 aaactaaaaa tgatgaatac aatttgtact gaattcatga acttgtcgat gttttcaggt
     4261 tttattggcg acttacctat aaacagataa attgcaagag ttgctattgc catactgagc
     4321 atctgacctg taagcctcat ggttcccaaa aaagcagagg caacgccata ttgtggtttt
     4381 tcaacagaac tcattactga gtttgtgttg ggtgaggcaa atattccaaa gccaagtcca
     4441 accacaaaca ggcatacaac aattgtggca aacccgctgt catggtgaag aaaaatcaat
     4501 gcaaatagtc cggatgcact gattgccatt cctaatgaag ctagtaaacc tgcctgaatc
     4561 ctgtcggata ggtgtccggc aaatggtgtg actattgcca taaccaatgg ttgcgcgagc
     4621 aacacaagcc cggcttcgcc cgggcttagc cctttaacgt attgaagata caaactcaga
     4681 ataaaactaa ctgcaaaagt agctgcatag ttaatcaggg cagcgaggtt cgaaaaagca
     4741 aacaccctgt tatgtctaat tagttttatg ttcattacag gggaagtaat tctgtattcg
     4801 atgattacaa aaaaaaccat tccggcaaca cctatggcag tcataataat agcaaaagct
     4861 tctgtaattc gggaaagacc gtacataagc aaactcatcg aaatcaaata cagcaacgat
     4921 cctgaaaggt cgaacttttc ccgggttaag ttttgatgtt gttcaacaat aacccgattt
     4981 gcaatggtaa tcactatcaa gcaaagcatc atagtcagta cgaaaatact tcgccaaccg
     5041 ataaattgtg tcattaaccc acccaaaaca ggtgctgctg aaaggccaat gtaaaccgaa
     5101 gcgcttataa gaccaattgc cctgcctctt ttttcgggag cgaaggcagc aaccacaata
     5161 gccatacttg tactgaaaac catagaactt ccaattccct ggattactct cgacgatata
     5221 agcatccccg ccgattgcga aactgaacat aaaagactcc caatggcaaa gatgatcata
     5281 ccggcaatga agagcttttt tctgccggtt atgtcggagt actttccaaa gggaaccaga
     5341 aatactgcag aagcgaggat atacgacatg gcaacccagc tcattgtaga ggcattcatt
     5401 gtgaattccc ggcttataac cggaagcgca acattcaccg atgctcccat aaatggggtg
     5461 atgaacgcag caagcatagc taccgtaatt aatcctgtac ctgaactttt ctcagaatgt
     5521 gagacatgta gtttttctgc caccgatgta tgtcaataat gctttaataa gacagttgca
     5581 attgcggtat catcccaaat gccgaaagaa tgagt
//
LOCUS       DAOOQP010000155         5532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_17742_length_5532_cov_4.105715, whole genome shotgun sequence.
ACCESSION   DAOOQP010000155 DAOOQP010000000
VERSION     DAOOQP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17742_length_5532_cov_4.105715"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLK75_11895"
     CDS             complement(<1..407)
                     /locus_tag="PLK75_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39990.1"
                     /translation="MKALFVMILLAVGISSFSQSAKDARLNRLKSKTTMKTVENNGVP
                     TIFKETYEEFDKNGKTTLKTEYSKSGSVKHQETYKYDSFGNVTEYVESDTKDGTVFKT
                     VFTYNANGDKVSETITDVSGKLVEKSTYTYDSKG"
     gene            complement(425..835)
                     /locus_tag="PLK75_11900"
     CDS             complement(425..835)
                     /locus_tag="PLK75_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY39991.1"
                     /translation="MKYRLLLAQCAVLIIVAATIASCEKPEGEGGTATINGSVWVRDY
                     NSTFTQLTGEYAAADEDVYIVYGDHVGYDDKTSTDYNGRFSFRYLRPGDYTVYVYSKD
                     STLTSVSGEIAVIQQFQVTQREQVVELPQFIIFN"
     gene            complement(893..3181)
                     /locus_tag="PLK75_11905"
     CDS             complement(893..3181)
                     /locus_tag="PLK75_11905"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY39992.1"
                     /translation="MLQFASQRFSWYLGTVLLFFALIEFPAVAFSQSDFYNIRKVQEI
                     RINFYDAHWKTTLDSLFAAGNSDIRIGADVTINGTVFHRCGVRYKGFSSWNSSETKNP
                     FNIDLDFSIKNQNYQGYTTLKLSNGIYDPSFIREVLSYYIVRQYMPASQANFSELYIN
                     DTLIGLYTNVEAVNDEFIEKHFGSCKNTFFKGSPEALDFPFGDNANLSYIEGEDSTYY
                     MTFYKMESEYGWSRLFELIRILNSDTASLPSVLNIDRTLWMHAFNYSLLNLDSYIAYS
                     QNYYMYEDDNSVFNPIVWDLNMSFGSFRYSDGALNYNGVTINKLKTLNPLQHLTFSIS
                     PRPLMKNLFLNSTYRKMYLAHIRTIINRNIISDEYLQIAQELHDTIAFYVDRDTNKFY
                     PYEYFQNNLTSTVGASGEQYPGIQDLMEARATYLSTYSGIGGYPLIDTPVCNPARPGN
                     GSIVNITTVVEGATEVYLYYRFHPALVFSKAEMSDDGLHNDGTSDDGIYGITLTIGGE
                     TLEYYVWAENETAGAFLPEDASYKFFTANVYPVSGRLVINEYGFCGANSIEQCVNLPA
                     WIELFNNSQVDINLKSFTLEQNDNVWYFTDTAIPANSFLCVSADAAFAESETNLGFFL
                     SCDCLMLKSDSGEIIDSCVVICPKAGNSVGRYPNGSGDFRLLLPSMASFNIPVTLNLP
                     VLYVSPNPASKYVIVQGEISGNQMNLKIFNQLGGLVYSKQFNSCNTFFAEKIELNGFA
                     SGEYIILLMDGTKYAFVKFIVV"
     gene            complement(3220..4446)
                     /locus_tag="PLK75_11910"
     CDS             complement(3220..4446)
                     /locus_tag="PLK75_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39993.1"
                     /translation="MKSSKTLALTLLVSLITLGAFAQLSVIQYTETNGLPSNDVCGGI
                     AVAPNGNIWAGTSAGVAKFNGTVWTGYDSGDGMISDYCTCIAVDNNNIVWVGTDMGLC
                     RFDGSTFTNYTTVEGLADNSVNYIACDNAGKVYIATFNGVSVFNGISFDNLTTDDGLP
                     TNLIVYVHVVAPNNVWFATLGEGAVYFNGTSFTSFSTGQGLASTDISSVVVDTDQKKW
                     IASFSGIDVLDSENQYYTLQNHEDGLYSPYADGLYHDFVQDVAADALGNIWACVYVDY
                     LQDGAVTLYSNGEWQTVFTETNSLRDIKTDPNGNAWIGSGAGVFKVQIESSLNEIDES
                     LMPVVYPNPAEQYINILYADKICSVKIFNVQGKLVSSYSNEQSNRFDISGLADGYYYI
                     QFDAANLRYTLNFVKK"
     gene            complement(4453..>5532)
                     /locus_tag="PLK75_11915"
     CDS             complement(4453..>5532)
                     /locus_tag="PLK75_11915"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39994.1"
                     /translation="PTYTYAWSSTNGYSGATQTPVIYPTVTGTYTVTITNTAISCSST
                     ASVTVTVLPLPEANAGNDVAINAGSSTTLTASGGGTYHWSTGANTQSITVNPAETTTY
                     TVTVTGSNGCTASDAVVVNVNAGTLSVAATADNQTICIGDPVQLYATPSGGAGYSYSW
                     SSVPEGFYSNEQNPVVYPVENTVYTILVTSGVNNASGSVNITVNPLPPTPTITEYSNF
                     LMSSAAYSYQWYLNGIPMDGAVNQVFTPLEPGNYQVEIIDNNGCSSMSAVFLFTGTSN
                     TSIDKESLITVYPNPSSGKVCIAIEDESIVNYTVKIISLTGANLLQGENLTELNLMNL
                     CTGIYYVYIYPENKNSIVKRISIIK"
BASE COUNT         1691 a         1172 c         1030 g         1639 t
ORIGIN      
        1 cccttggaat cgtaggtata tgttgatttt tcgacaagct tacccgacac atcggtaatt
       61 gtttcggaca ctttatctcc gtttgcattg taagtaaaca cggttttaaa cactgttccg
      121 tctttggtat cggattctac atactcggtt acgttaccga agctatcgta tttatacgtt
      181 tcctgatgct taacagagcc acttttcgaa tattctgtct taagggtagt tttaccgttt
      241 ttatcaaact cttcatacgt ttctttaaaa attgtcggta ccccattgtt ttcaacagtt
      301 ttcatcgtag ttttcgactt caacctgttt aacctggcat ccttagcact ttgagagaag
      361 ctgcttatgc caacagcaag taaaatcatt acaaataatg ctttcatatc aacaataatt
      421 tacattaatt aaaaataata aattgaggta attctactac ctgttctctc tgggtaacct
      481 ggaattgttg aatcacggca atttctcccg ataccgaagt cagtgttgaa tcttttgaat
      541 aaacatatac cgtataatca ccaggcctca ggtaacgaaa actaaaccgg ccgttgtaat
      601 cggtggaagt tttgtcgtcg tagccaacat ggtctccata cacaatatac acatcctcat
      661 cggcagcagc gtattctccg gtaagctgtg taaaagtact gttataatca cggacccaaa
      721 cactcccgtt aatggtagcg gttccgccct ccccctccgg tttttcgcaa gaggctatgg
      781 ttgcagccac aataataagt acagcgcatt gggctaataa taaacgatat ttcatctttc
      841 tatgttttaa attttgttta caaatctaat aaatcaatgt cttaaaaacg cattaaacaa
      901 caataaattt cacaaacgca tattttgtgc catccatcag gagaataatg tattctcctg
      961 atgcaaatcc atttaattca attttttcgg caaagaaagt attgcaactg ttgaactgct
     1021 ttgaatagac aagtccgccc aactgattaa atatcttcag gttcatttga tttcctgata
     1081 tctcaccttg aacaatcaca tacttactgg ctggatttgg gcttacataa agcaccggca
     1141 agttcagtgt aactggtata ttgaatgaag ccatcgaagg gagcagtaac ctgaagtcgc
     1201 cactaccatt ggggtacctt ccgactgaat tccctgcctt agggcatatt actacacaag
     1261 aatcaataat ctctccgctg tcgcttttca gcataaggca atcacacgaa agaaagaatc
     1321 ccaaatttgt ttccgactcc gcgaaagctg catctgccga cacacataaa aaagaatttg
     1381 cagggattgc agtatccgta aaataccaaa cattatcgtt ttgctctaaa gtgaatgact
     1441 tcaggttgat atcaacttgc gaattgttga ataattcaat ccatgccggc agattaacac
     1501 attgttcgat ggagtttgca ccgcagaaac catattcatt aatcaccaat ctccctgata
     1561 cgggataaac atttgcagta aaaaatttat acgaagcatc ttcaggaaga aatgcaccgg
     1621 cggtttcgtt ttcagcccag acatagtact ccagtgtttc tccaccaatt gtgagggtta
     1681 taccatagat tccatcatcg gaagttccgt cgttgtgcaa tccgtcatcc gacatttcag
     1741 ctttgctaaa taccaatgcg gggtggaaac gataatataa atacacttca gttgccccct
     1801 caacaacggt tgtaatattt acaatactac catttcccgg tctggcagga ttgcaaaccg
     1861 gagtatcaat aagtggatat ccgccaatgc ctgaatacgt gctcagatac gttgcacgtg
     1921 cttccatcag atcctggatg ccgggatatt gctctcctga agcacctacg gtagatgtaa
     1981 ggttgttttg aaaatattcg taagggtaaa atttgttagt atcacggtca acataaaaag
     2041 caattgtatc gtgcaattcc tgagcaattt gcaagtattc atcactaatt atattcctat
     2101 ttataatagt ccgtatatgg gccagataca ttttacgata cgtgctgttc aaaaacaaat
     2161 ttttcatcaa cggtcttggt gaaatcgaaa aagtaaggtg ctgcaaaggg tttaaggtct
     2221 taagtttgtt tatggttact ccattatagt tcaaggctcc atcgctataa cgaaaacttc
     2281 caaacgacat gttcaaatcc cagacaatcg gattaaagac tgaattatcg tcttcgtaca
     2341 tatagtaatt ctgtgaataa gcaatataac tatccaaatt aagcaatgaa taattaaaag
     2401 catgcatcca caaagtgcgg tcgatattca aaacagacgg gagacttgca gtatcggaat
     2461 tgaggattcg tatcagctca aacagtctgc tccaaccata ttccgattcc attttataaa
     2521 aagtcatata ataggttgaa tcctcaccct caatgtaact gagattggca ttgtcgccaa
     2581 acggaaaatc gagggcttcg gggctacctt taaaaaacgt gtttttacaa cttccaaaat
     2641 gcttttcaat aaactcgtca ttcactgcct caacattggt atataagcca atgagtgtgt
     2701 cgttgatata caattccgaa aaattggctt gggaggctgg catatactgg cgtacaatgt
     2761 aatacgacaa tacctccctt ataaatgaag ggtcataaat accattgctc agcttcaggg
     2821 ttgtgtaacc ctgataattc tgatttttta tactgaagtc aaggtcaatg ttaaacggat
     2881 ttttggtttc ggatgaattc catgaactga agcctttgta gcgaactccg cacctatgaa
     2941 atacagttcc gttgattgta acatctgccc cgattcgaat gtcgctgttt cctgcagcaa
     3001 acaagctatc gagagtagtt ttccaatgag catcgtagaa attgatacga atttcctgca
     3061 ctttcctgat attgtagaaa tcgctttgtg agaatgccac tgcgggaaat tcaatgaggg
     3121 caaaaaacaa aagtactgta cctaaatacc atgaaaatcg ttgagaagca aattgcaaca
     3181 cttggtctga ttataaatac tgctggttaa ttcaaatact tattttttca caaagttaag
     3241 ggtataacgc aaatttgctg catcgaattg aatatagtag tatccatcag ccaaacctga
     3301 aatatcgaaa cggtttgatt gctcattact ataactgctg accagtttcc cctgcacatt
     3361 aaatatttta acagagcata ttttatcagc atataagata ttgatatatt gttctgcagg
     3421 gtttgggtaa acgacaggca tcaggctctc atcgatttcg ttcagacttg attcaatctg
     3481 aactttaaaa acacctgctc ccgaaccaat ccaagcattg ccattcggat cggtttttat
     3541 atctcgcaac gaatttgttt cggtgaatac agtctgccac tcgccattac tatacaacgt
     3601 aactgcgcca tcctgaagat aatcgacata aacacaagcc cagatattcc caagcgcatc
     3661 agctgcaaca tcctgaacaa aatcatgata taagccatca gcataaggtg aatacaagcc
     3721 atcttcatga ttttgcaatg tataatactg attttcggaa tccaacacat ctattccgct
     3781 aaaagaagca atccacttct tttgatctgt atcaaccaca acagagctga tatcggttga
     3841 ggcaagtcct tgtccggtgc tgaaagatgt aaaagatgtg ccattgaaat acacggcacc
     3901 ttcgcccaag gtagcaaacc aaacattatt tggggcaaca acatgcacat aaacgatcag
     3961 attggtgggc aggccgtcat cggttgtaag attatcgaac gatatgccat taaaaactga
     4021 aacaccattg aatgttgcaa tatatacctt accggcatta tcgcaagcaa tgtaatttac
     4081 cgaattatcg gccagacctt caacagtggt gtagttagta aaagtagatc cgtcgaatcg
     4141 acaaagcccc atatcggtgc caacccatac tatgttgtta ttgtccaccg caatacaggt
     4201 gcaatagtcg cttatcatac catctccact atcgtagccc gtccatacag taccattgaa
     4261 ttttgcaact ccagctgaag tacccgccca aatattccca ttcggtgcaa cggcaatgcc
     4321 accgcaaaca tcgttactgg gtaatccgtt tgtttcagta tactgaataa ctgatagttg
     4381 cgcaaacgca cctaaagtta tgagcgaaac caataaagta agagcaagtg ttttcgaact
     4441 tttcatacga ttttatttta taattgaaat acgcttaaca atagaattct tattttcagg
     4501 ataaatgtac acataataaa ttccggtgca caggttcatc aaattgagtt cagtgaggtt
     4561 ttcaccctga agtaaatttg ctcctgtaag tgaaatgatt tttactgtat aatttacaat
     4621 gctttcatct tcgatagcaa tacatacttt tccactggaa gggttcgggt atacagtgat
     4681 gagactttcc ttatcaattg aagtgttact tgtacctgta aataaaaaaa ctgctgacat
     4741 ggatgaacac ccattattat caataatttc gacctgataa tttccaggct ccagtggcgt
     4801 gaatacctga ttaactgcac catccatagg tattccattt aaataccatt gataagagta
     4861 tgctgctgac gacatcagaa aattgctgta ttcagtaatc gtaggggttg gcggcaatgg
     4921 gtttacagtg atgttcacac ttcccgaagc attgtttaca ccactggtta ctagaatggt
     4981 gtagacagta ttttcaacag gatagacaac cggattttgc tcatttgaat aaaacccttc
     5041 aggcaccgat gaccaactgt aagaataacc ggcccctcct gatggggtgg catatagctg
     5101 aacagggtcg ccaatacata tcgtttgatt atcggcggtc gctgcaacgc taagtgttcc
     5161 agcattaaca tttacaacca cagcgtcaga tgcagtacag ccatttgaac cggttacggt
     5221 cactgtataa gtagtagttt ctgcaggatt tacagtaatg ctctgagtat ttgcacctgt
     5281 actccagtga taggttcccc ctccagatgc tgtaagggtt gtagaagatc ctgcattaat
     5341 agcaacatcg tttcctgcat tagcctcagg taaaggtaat actgtaacgg taaccgacgc
     5401 agtgcttgaa caagaaatgg cagtgttggt aatagttacc gtataagttc ccgttactgt
     5461 tggatatatt accggggttt gggttgcacc tgaataaccg tttgtcgaag accatgcata
     5521 cgtgtaagtt gg
//
LOCUS       DAOOQP010000156         5346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_18473_length_5346_cov_3.966358, whole genome shotgun sequence.
ACCESSION   DAOOQP010000156 DAOOQP010000000
VERSION     DAOOQP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18473_length_5346_cov_3.966358"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="PLK75_11920"
     CDS             <1..1615
                     /locus_tag="PLK75_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY39995.1"
                     /translation="INAVTIYNIQVNNDLIISAATQFNCNGNTMYIGGNWAKTSGQFN
                     RGTGTVYLTGTGKLISGNETIFRNLNISGSYTLQAAQLTAMRTSNVGGDITIASGGSL
                     NCQSNSISLEGNWNNDGSFTAGTGLVRFNGSLQQSIQPGSSVFNNVVFINTTAGSADL
                     NIGEPMVINGAATFTSGIAYFSGTGSLTFGSSATSNDGDDDSYVDGLVAKTGTTAFTF
                     PVGDGIVWAPVRVSAPVSSATITAQYNFVAPELNWSAAYMCNESLMQYTSGVENWNLT
                     TTGPTPSVTLYWKNGTRSGITNLSDLAVAHFNGSCWEYLEGSTAGNVSSGSITSTIPF
                     TSYSPVSFGSKRRINPLPVELINFNATCNSGVVNINWQTASETNNEKFIIERSVNSVD
                     FVEIGEVKGAGNSNVHNDYSYIDNYHDGGQRYYRLKQTDFNGKFEYSDIVVVTCKGND
                     EFSPELSAHPNPFNTELIVSGTNIPSESAMLIVEDVLGKAILSKDIPVNSGSFIVQLQ
                     MTDLPPAVYFIKVISQGYSKTIKVVKEQD"
     gene            complement(1673..1748)
                     /locus_tag="PLK75_11925"
     tRNA            complement(1673..1748)
                     /locus_tag="PLK75_11925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1713..1715),aa:Lys,seq:ttt)
     gene            2042..>5346
                     /locus_tag="PLK75_11930"
     CDS             2042..>5346
                     /locus_tag="PLK75_11930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY39996.1"
                     /translation="MKRLIISLLVVVFSVSAMNVFAQLGHGGEPISFKNPDLLSTYID
                     HIQLAKPDMALIEAEDNHSDKDGTLYMIGRFIDVDRDINDCGTWDVLPNGTRVWRLKI
                     SVPDAKALSVFYDNFYLPQGTQLFIYNQNHKQLVGSLDYRNNPRFSNMYSTEIIEGET
                     TYLELIVSPEATELPAIEIGEVCYIYRGVDPLIGRFMDSKPPTFGGSGACEVNVNCPE
                     GSGWQDQKKGVAVIFCNGGLCSGTLVNNTSNDGTPYFLTADHCGGSTGTVPNWQYYFN
                     YEGSGCTDPASAPAYNTITGSTLRSRGDEDTGSDFLLVELNCTEDELETIGAYYNGWD
                     RSTTGSPSGVSIHHPDGDIKKISSYNTPCVSGTFNTCPANAHWKAVWYNAGTSLGVTE
                     PGSSGSPLFNNNKLVVGTLSGGPSSCGVAAANCYDLYGKFDYHWETNGTADNVRLKPW
                     LDPTSSGATTCPGRFPNSGASSINAQFIGTPTTVNVGGTVAFTDQSTGGATTWSWTFD
                     GGTPGTTMNQNPTVNYPTAGTYNVTLTAGNGTETDTEIKTGYIIVTSGASTLDAAFAA
                     SAYNIFEGECINFQDQSTGNPTSWAWTFPGSNTPTSPDQNPVNVCYNVAGTYNVSLTV
                     QNATDTNTENCTGCITVVVNPVNPIADFSGSPLIIPVGGVVTFTNLSQNGPFIEPWAW
                     TFEGGIPNVSNDSVPQPIMYNTVGTYDVELLCSNTLGVQDIELKHDYIKVIPAATEPP
                     VANFVANYTVIQPGDAINFIDLSLGNPYQWEWTIQGGDPSDPTVQNPSNVIFALPGIY
                     DVQLIVRNNLGVDTLIREDYIVVSATDPCVTAPVASFTAAPRLITAGQVVYMQDQSTG
                     LPANWNWIFEGGNPQFSTEGSITEGVLYSVPGIYNVTLAVNNICGSDMLIKDDYIYVF
                     SGPVTLFCDTLSNIGPNETVGTIVPQDAYGFIAGHNGERVRAYADYFEDYTFGQIESL
                     IVPVERAVYGSYNSYVRFMIWDWDGDTIGEVLGEKKVYIRDLQANFNNVITFNPPVEI
                     DGPFYAGFRLNYPDENNDGYCDDQFVVSIANPRGANESYNTMYVLDGIVWKSNVEMFG
                     FATSLAIKPLGCLV"
BASE COUNT         1536 a         1059 c         1171 g         1580 t
ORIGIN      
        1 tattaatgcc gtaactatct ataacattca ggtcaacaac gatctaataa tatcagctgc
       61 aacccagttc aactgtaatg ggaatactat gtatatcggg ggtaattggg cgaaaacaag
      121 cggccagttt aaccgaggta ccggcactgt gtaccttacc ggaaccggca agcttatctc
      181 gggaaatgaa accattttca ggaacctgaa catatcaggc agttatactt tgcaagctgc
      241 tcagcttact gcaatgcgaa catccaatgt cggtggcgat ataactattg caagtggagg
      301 ttctcttaat tgtcaatcga attctataag cttagagggt aactggaaca atgacggaag
      361 ctttactgcg ggaaccggat tggtacgttt taatggcagt ttgcaacaaa gcatacagcc
      421 tggcagcagt gtgttcaata atgttgtttt tattaacaca actgctggtt cggctgattt
      481 aaatattgga gaaccgatgg ttataaatgg agctgctact tttacaagcg gtattgcata
      541 tttctcagga accggatctc ttacttttgg aagttctgct acgtcgaacg atggtgatga
      601 tgatagctat gttgacggtc tggtggcaaa aacagggaca acagcattta ctttccctgt
      661 aggcgatggt attgtttggg ctccggttag agtttcagcc cctgtaagca gtgcaacaat
      721 aacagcacag tataattttg ttgctccgga gttaaactgg tcggcagcct atatgtgtaa
      781 cgaaagcctt atgcagtata ccagcggtgt tgaaaactgg aaccttacta ctacagggcc
      841 tacaccatct gttactttat attggaagaa tggtacacga agtggtatta ccaatctctc
      901 agatctcgct gtagcacatt ttaatggttc ttgctgggag tatttggaag gatctactgc
      961 agggaatgta tcatccggtt cgataacttc aaccattccg tttacatcat atagtccggt
     1021 aagctttgga agcaagcgca gaattaatcc actacccgtt gagttaataa atttcaacgc
     1081 tacatgtaat tcaggtgttg tgaatatcaa ctggcagaca gcttcggaga caaataacga
     1141 aaaatttata atcgagcgtt ctgtcaattc agtcgatttc gtggaaattg gagaagttaa
     1201 gggagcaggc aacagtaatg tgcataatga ttattcatat atcgataatt atcacgatgg
     1261 agggcagcgc tactacaggc ttaaacagac tgattttaac ggaaaatttg aatattccga
     1321 tatcgttgtt gttacatgca aaggcaatga tgaattttcc cctgagcttt cagcccaccc
     1381 gaatccgttt aatacagagt tgattgttag tggtactaat ataccctcag agtctgctat
     1441 gcttattgtt gaagatgtgc ttgggaaagc aatattaagc aaagatatac ctgtgaattc
     1501 gggcagcttc attgtgcaat tacaaatgac agaccttcct ccggcggttt acttcatcaa
     1561 agtaatttcg caaggatatt ccaaaactat taaagttgtt aaggaacagg attaaacagc
     1621 aacagaaata ttcatttaat gaaaaaagcc ggcattaaac cggcttttcg tttggtgact
     1681 gattggagac tcgaactcca gaccctctgc ttaaaaggca gatgctctac ctgctgagct
     1741 aatcagtctt ttcaattccc ctgaatttgg gactgcaaag atatgttttt gatttttaaa
     1801 aaaccaaaca ttattgataa aataaatcaa tttattttta ttcaagcatt ctaagcccaa
     1861 atcttatgct ggagcctgat tatttaatgc aatagagaaa ggtttgtttc aaacataata
     1921 caaaggcaac cgaatttaac cgcatcagtc ttattaaaat aaaattattg tacgcttaat
     1981 ttaaaaaaaa tgtataaatt gcaggttaat acactgctaa gtatgtaatt atatatttac
     2041 tatgaaaaga ttgataatca gcttgttagt tgttgtgttc tcagttagcg caatgaatgt
     2101 ttttgcacaa ttaggtcatg gtggtgagcc aattagtttt aaaaatcctg atttactcag
     2161 cacttatatc gaccatattc agcttgcaaa acctgatatg gctcttattg aagccgagga
     2221 taatcactct gataaagatg gaacgcttta tatgattggt cgttttattg atgtcgatcg
     2281 ggatatcaat gattgtggaa catgggacgt tttgcccaat ggtaccagag tttggcgtct
     2341 gaaaatatcg gtgcccgacg ccaaggcatt gagcgtgttt tacgataatt tttatcttcc
     2401 acaaggaact cagcttttta tttataatca aaaccacaaa cagcttgtgg gatcgcttga
     2461 ttatagaaat aacccccgtt tcagtaatat gtattccacc gaaattatcg aaggcgaaac
     2521 aacttatctg gaactgatag tttctcctga agctactgaa ttgcctgcaa tagaaattgg
     2581 agaggtttgc tacatatatc gaggagtcga tccattgatt ggtaggttta tggatagtaa
     2641 accacctact tttggtggtt ctggtgcttg cgaagtgaat gtgaactgcc ctgaaggatc
     2701 cggttggcaa gatcagaaaa aaggcgttgc tgtaattttt tgcaatggag gtctttgttc
     2761 cggaactctg gttaacaata cttcaaacga cggtacacca tattttctta cagctgacca
     2821 ttgcggtggt tctacaggca ctgtacccaa ctggcaatat tatttcaatt acgaaggatc
     2881 aggttgcaca gatccagcct cagcacctgc ctacaacact attacaggtt ccactctcag
     2941 atccaggggc gatgaagata caggctccga tttcctttta gtggagttaa attgcactga
     3001 ggatgaactt gaaacgattg gtgcatatta caacggttgg gacagatcga caaccggcag
     3061 cccaagtggc gttagtattc atcaccctga tggcgacata aaaaaaatat catcctataa
     3121 tacaccgtgt gttagcggaa cattcaatac ctgccctgcc aatgcccatt ggaaagctgt
     3181 ttggtataat gcaggaacca gccttggtgt aactgaacct ggttcttcag gctcaccttt
     3241 gtttaacaat aacaagcttg ttgtaggtac tctcagtgga ggcccttcct cttgcggtgt
     3301 tgccgcagcc aactgttacg atctttacgg gaaattcgac taccattggg aaacaaatgg
     3361 tactgccgat aatgttcggt tgaaaccttg gcttgatccc accagcagtg gggctacaac
     3421 atgccctgga agattcccca atagcggagc ttcttcaatc aatgcacaat ttattggaac
     3481 gcctactact gttaatgtcg gtggtactgt tgcttttacc gatcaatcga caggaggcgc
     3541 aacaacatgg agttggactt ttgatggggg aaccccgggt acaacaatga atcaaaaccc
     3601 aactgtgaat tacccaacag caggaaccta caatgttaca cttaccgcag gcaacggaac
     3661 cgaaacagat actgaaatta aaaccggtta tataattgtt acaagcggag catccacact
     3721 tgatgccgct tttgctgcct ctgcctacaa tatttttgag ggtgaatgta ttaattttca
     3781 ggaccagtca acaggcaacc ccacttcatg ggcgtggact ttccctggat cgaatacacc
     3841 cacatcgccc gaccagaacc ctgtgaatgt ttgctacaat gttgcaggaa cctataacgt
     3901 aagtctgaca gtgcagaatg ctactgatac aaatactgaa aactgcactg gttgtattac
     3961 ggttgttgtt aaccctgtaa atcccattgc cgatttttca ggttcaccat taatcatccc
     4021 cgtaggtggt gtggttacat tcaccaattt gtcgcagaac ggacctttca ttgaaccctg
     4081 ggcatggact tttgagggcg gtatccccaa tgtttcgaac gattcggtac cgcagccgat
     4141 tatgtataac acagttggta cttacgatgt ggaattactt tgcagcaata cgcttggtgt
     4201 tcaggacatt gagctgaagc atgattatat taaggttatt ccggcagcta cggagcctcc
     4261 tgttgccaat tttgttgcta actacacggt tattcaaccc ggtgatgcga ttaatttcat
     4321 tgacttaagc cttggaaacc cgtatcaatg ggaatggacc attcagggtg gagacccctc
     4381 tgatcctacc gttcagaatc cgagtaatgt tatttttgcc ttacccggga tttatgatgt
     4441 tcaattaatt gtgagaaaca atttgggtgt tgatacttta atcagggaag attacattgt
     4501 tgttagtgcc accgacccct gtgttactgc acctgttgct tcctttacag cagcaccccg
     4561 attaattact gcaggtcagg tagtatatat gcaggatcag tcaacaggat tgcctgctaa
     4621 ctggaattgg attttcgaag gtggaaaccc gcagtttagt accgaaggca gtattacaga
     4681 aggagtactt tactcggtac ccggaattta taacgtaacc cttgcagtta ataatatttg
     4741 tggaagcgat atgcttatta aagacgatta tatttatgta ttctccggac cggtcactct
     4801 gttttgcgat acattgagta atatcggacc caatgaaacc gtaggaacaa ttgttccaca
     4861 ggatgcttat gggtttattg ccggccataa cggggaaaga gtgcgtgctt atgctgatta
     4921 tttcgaagac tatacatttg gtcagattga gtcgctgatt gtacccgtcg aaagagcagt
     4981 ctatggctcg tacaattcat atgttcgttt tatgatttgg gattgggatg gcgacaccat
     5041 tggcgaagtg ctaggcgaga agaaagtcta tatccgtgat cttcaggcaa attttaataa
     5101 tgtaataacc tttaatcctc cggtcgaaat tgatgggcct ttttacgccg gtttccgttt
     5161 gaattatcct gacgagaaca acgacggata ttgcgacgat caatttgtag ttagcatagc
     5221 aaacccaagg ggagcaaatg aatcgtacaa tacaatgtac gtgcttgatg ggattgtgtg
     5281 gaaatcgaat gtagaaatgt ttggttttgc cacatctctg gctattaagc ctttgggttg
     5341 tttggt
//
LOCUS       DAOOQP010000157         5295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_18725_length_5295_cov_4.386260, whole genome shotgun sequence.
ACCESSION   DAOOQP010000157 DAOOQP010000000
VERSION     DAOOQP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_5295_cov_4.386260"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PLK75_11935"
     CDS             <1..1384
                     /locus_tag="PLK75_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY39997.1"
                     /translation="PILTGFSNTEINNVLAHSEAKCILVSDKYRSKIDIKDLPKLQSI
                     VSIDDFSVVSSPVKGVYYQYGKFENNGFKVYEDDLAAIIYTSGTTGKSKGVMLSHLNI
                     TFNAHSAGILQPINNKDRFLSLLPLSHTYENTLGFILAIMNGACVYYLKQSAAPSVLI
                     QAMQTVRPTIILTVPMIIEKIYKSRIAPSFSKSKIMRSLYKNKNLKLILHQIAGKKLM
                     KSFGGKLRFFGVGGSKIDPVVEQFLLDAKVPIAIGYGLTETAPLVAGTNPQTARHQST
                     GPAITGVEIKIHNQNPQTGLGEIWVRGKNVMMGYYKDAAQTEEVITPDGWFRTGDLGM
                     LSNDNYLYIKGRNKTMILGSSGENIFPEDIESIINKYDHVVESLVVEKNGSIVALVHF
                     NKEEIEQKYHDLYGKISEYTDDTIEILKKELHQYINKQVSKYARVNRIEAQNKPFEKT
                     ATQKIKRYLY"
     gene            1445..1999
                     /locus_tag="PLK75_11940"
     CDS             1445..1999
                     /locus_tag="PLK75_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOY39998.1"
                     /translation="MIEWILIALALNADCLAVSIATGYCIKEDKLISSLRIAIVFSII
                     QTLLPLLGWVGGMQLGSVISRFDHWIAFALLVFIGIRMVWGAAKEGNYRKTVDPDNIL
                     LLIVLGFATSIDALAVGVSLGLIDTPIWLPLAFIFGVTFFVSFGGALTSVFLSKHLKF
                     KMEYVAGVILAGLGTRILITHLIA"
     gene            2116..3189
                     /gene="serC"
                     /locus_tag="PLK75_11945"
     CDS             2116..3189
                     /gene="serC"
                     /locus_tag="PLK75_11945"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOY39999.1"
                     /translation="MRKHIFNAGPCKLSDRTLENTAKAVLELGNSGQSIMEVSHRGKD
                     FEAILADTVALFKEILEIPEGYSVLFLGGGASMQFCMIPYNFMKSKAMYINTGTWATA
                     AIKEAKIWGEVVEISSKDREFKYIPKGYAIPDDVEYLHITTNNTIKGTELLKDLDVKV
                     PLIADMSSDIMSRKVDVSKYVMIYGGAQKNLGPAGVTFVIIKNEYLNKLVDRPIPTML
                     KYSTHIEGGSMYNTPPCVNIYALRETLYWIKEMGGLEVMNRMADERANYLYNAIDSSK
                     MFVGTVDKEDRSRMNICFVMQEAYKHLEDDFIKFASSRNIVGIKGHRSVGGFRASTYN
                     ACTMDDVKALVNAMKDFEKEHVK"
     gene            complement(3236..3655)
                     /locus_tag="PLK75_11950"
     CDS             complement(3236..3655)
                     /locus_tag="PLK75_11950"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOY40000.1"
                     /translation="MKSLYLIPIAIFSGITLFAQTTEVNPIDSMYYACLENPDNYSTA
                     GSIQCADRAFYEWSLEMEKNLVLLKEVLNETEFVLLTESQQHWYAFQNSETTFSNTMH
                     ANMDGTMFQIFAADIRVQIVRHRALALRDYYDTKSIK"
     gene            3800..4483
                     /locus_tag="PLK75_11955"
     CDS             3800..4483
                     /locus_tag="PLK75_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40001.1"
                     /translation="MKAKIILYVVILAAVFQQSCAPCYVPNVVNSPMLTTDKQLNASV
                     HGGTSGFDMQTAYSPAEHWGVMLNGSYMNQTADSSDDYHKHIFAEAGVGHFVNLGKFG
                     LLDAYAGAGMGRINSLQTSGIFGSEAHVMSARVFIQPSVSFVTDYFQSSLSLRTVMVH
                     IWQGAVSDNGYFFEPTLTIKVGVPAIKLVAQAGLSLPANSHHVSFNYEPFIVSVGLQT
                     QFNTGYKKR"
     gene            complement(4517..>5295)
                     /gene="glyA"
                     /locus_tag="PLK75_11960"
     CDS             complement(4517..>5295)
                     /gene="glyA"
                     /locus_tag="PLK75_11960"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOY40002.1"
                     /translation="AYSREWDYKRMREIADKIGAILMVDMAHPAGLIAAGLLENPCKY
                     AHIVTSTTHKTLRGPRGGIILIGKDYDNPWGKTTPKGVMKKMSEIINSAVFPGMQGGP
                     LEHVIASKAVAFGEALDPSFRVYQQQVKKNAAVMADSLIKKGYKVISGGTDNHSMLID
                     LRTKFPDVNGKQVENALVQAEITVNKNMVPFDHRSPFQTSGLRLGTPAITTRGLKENH
                     MDVIVEMIDTVISNFDKPEVIESTKKRVYDLMHAFPLFAW"
BASE COUNT         1656 a         1036 c         1073 g         1530 t
ORIGIN      
        1 tccaatcctt acgggattca gcaacactga gatcaacaat gttttagcgc attccgaggc
       61 taagtgcatt ttagtatcag ataaataccg gtcgaaaatc gatattaaag atctcccgaa
      121 acttcaaagt attgtttcaa tcgatgattt ttctgttgtt tcaagtcctg tgaaaggcgt
      181 ttactaccaa tacgggaagt ttgaaaataa cggatttaaa gtttatgaag atgacctcgc
      241 cgcaataatt tatacatcgg gaacgactgg aaaatcgaaa ggtgttatgt tatcgcatct
      301 aaacatcact tttaatgcac attcggcagg gatacttcaa ccaattaata ataaggatcg
      361 ctttttatcg cttctaccgc tctcacacac ttacgaaaac actttaggat ttattcttgc
      421 cataatgaac ggcgcatgcg tatattacct gaaacaatcc gccgctccgt ctgtgcttat
      481 tcaggcaatg caaacagtaa gaccgaccat tatacttact gtaccaatga ttatcgaaaa
      541 gatatataaa agccgcattg caccttcatt cagtaagagt aaaataatgc gcagcttata
      601 taaaaataaa aacttaaagc taatcctgca ccaaattgcc ggcaaaaaat taatgaagtc
      661 atttggcggt aaattgcgat ttttcggcgt aggtggctcc aaaatcgatc cggttgttga
      721 acaatttctg cttgatgcaa aagtacccat cgccataggc tacggactta ccgaaacagc
      781 tccactggtt gcaggtacca atccgcaaac agcacgacat caatcaacag gcccagcaat
      841 caccggggtg gaaataaaaa tacacaacca gaatcctcaa acgggtctcg gtgaaatatg
      901 ggtacggggt aaaaatgtaa tgatgggata ctataaggat gccgctcaaa ccgaagaggt
      961 aattacccct gacgggtggt ttcgcaccgg cgatctggga atgctaagca acgataacta
     1021 cctttatatt aaaggaagaa ataaaaccat gatactgggc agcagcggtg aaaacatttt
     1081 tccggaagat atagaatcga taataaacaa atacgatcac gtggttgaat cgcttgttgt
     1141 ggaaaagaat ggttctattg ttgctcttgt tcatttcaac aaagaagaaa ttgaacagaa
     1201 ataccatgat ctatatggca aaatatccga atataccgac gataccattg aaatattaaa
     1261 aaaagaactg catcagtaca tcaataagca agttagcaaa tatgccagag tgaaccgtat
     1321 tgaagcacag aataagcctt ttgaaaaaac cgcaactcag aagatcaaga ggtatttata
     1381 ctaagcattg agttggcaat gatgcagcta tgcactcaac ccttcagaaa accagcatgc
     1441 aagcatgatt gaatggattc ttattgcgtt ggctttaaat gctgactgcc ttgcagtatc
     1501 aatcgcaacg ggctattgta tcaaagagga taagctaata tcatcactcc ggattgcaat
     1561 cgtattcagt atcattcaaa cacttttacc tcttttggga tgggttggag ggatgcaact
     1621 cggtagtgta atcagtcggt tcgatcactg gattgcattt gcactgcttg tgtttatagg
     1681 aataagaatg gtttggggtg ctgcaaaaga agggaattac cgcaagactg ttgaccctga
     1741 taatattctg ctgcttattg ttctgggatt tgcgaccagc attgacgcct tagctgttgg
     1801 agtatctctt ggcctaatcg atacacccat atggctcccc cttgcattta tttttggcgt
     1861 aacatttttc gtttcttttg gcggagcttt gacaagtgta tttttatcga aacatcttaa
     1921 gtttaagatg gagtacgtgg cgggggtgat actggcaggc ctcggtacca gaattcttat
     1981 tacccaccta attgcatagt aggttaaggt agtcctgact ttttaccgtg aaaccgtaca
     2041 ataagtatgt tataaatcat tattgttttt tcaacatttt tctacctttg agagaaatta
     2101 aaacacatat cgaatatgag aaaacacatt tttaatgccg gaccttgtaa attatcggac
     2161 cgaacattgg aaaacactgc caaagcagta cttgaactcg gcaattccgg gcaatcaata
     2221 atggaagtat cgcaccgtgg aaaagacttc gaagcaattc tagcggatac agttgcttta
     2281 ttcaaagaaa ttcttgagat ccccgaaggc tatagtgttt tattcctcgg aggcggtgcc
     2341 agtatgcagt tttgcatgat accctacaat ttcatgaaat cgaaagccat gtacattaac
     2401 acaggaacat gggctacagc tgctattaaa gaagcaaaga tctggggcga ggtagttgaa
     2461 atttcatcga aagacagaga gtttaaatat attcctaaag gatatgctat tcccgatgac
     2521 gttgaatatc tgcacattac aactaacaac actataaagg gcactgaatt attaaaagac
     2581 cttgacgtaa aagttccttt gattgctgat atgagttccg atattatgag ccgtaaagtt
     2641 gatgtttcga aatacgttat gatttatgga ggtgctcaaa agaatcttgg ccctgccggt
     2701 gttacttttg taattatcaa gaacgagtat ttgaacaaat tagttgacag acctatccct
     2761 acaatgttga aatactctac acatatcgaa ggaggttcta tgtacaatac tcctccttgt
     2821 gttaatatat acgcgttgcg tgaaaccttg tactggatta aagaaatggg tggattggag
     2881 gtaatgaacc gaatggctga tgaaagagcc aattaccttt acaatgcaat cgacagcagt
     2941 aaaatgtttg ttggtaccgt tgataaagaa gaccgttcgc gtatgaacat ttgttttgta
     3001 atgcaggaag catacaagca cctcgaagat gacttcatta aatttgcctc atcgcggaac
     3061 attgttggaa ttaaaggtca ccgttcagtt ggaggcttca gggcatcaac ctacaatgca
     3121 tgtaccatgg acgatgttaa agctcttgtg aacgcaatga aagatttcga gaaagagcat
     3181 gtaaaataaa atgaaaccat aaattaataa aaaaaaccgg cacaaaccgg tttttttatt
     3241 ttatcgattt tgtatcgtaa taatccctaa gtgctagtgc gcgatgcctg acaatctgta
     3301 ccctgatatc ggctgcaaaa atctgaaaca ttgtaccatc catgttcgca tgcatagtgt
     3361 tggaaaaggt agtttcacta ttctgaaaag cataccaatg ttgttgcgat tcagtaagta
     3421 aaacaaactc cgtctcgttt agtacttctt tcagcaaaac taaatttttc tccatttcaa
     3481 gtgaccattc ataaaatgcc cgatctgcac attgtattga gccggcagtt gaataattat
     3541 cagggttttc gaggcaggca taatacatcg aatcgatagg gtttacttcg gttgtttgag
     3601 caaatagcgt aatgcccgaa aaaattgcaa tagggataag atacaatgat ttcatttctt
     3661 gggtggtatt taacaagtaa agatacacta aaaataaaga cattatatta ttgtaacaat
     3721 aaagaaacaa agtaagatac accgtcatat gtatcgaaat taagcttata ttgtatgcac
     3781 tgaaaaaaat cagacaacca tgaaagctaa aatcattttg tatgtagtaa tattagcagc
     3841 agtttttcaa caatcttgtg caccatgcta tgtccccaac gttgtgaatt caccaatgct
     3901 aactaccgac aagcaactta atgcctctgt gcatggtggc acttcgggtt ttgacatgca
     3961 aacagcttat tcaccggctg aacattgggg tgttatgcta aacggcagtt atatgaatca
     4021 aacagccgac agcagtgacg attaccacaa acatattttc gcagaagccg gtgtgggtca
     4081 tttcgtaaat ctcgggaaat tcggattact cgatgcttat gcaggcgcag gaatgggtag
     4141 aattaattca ctacaaacct ctggaatatt cggatcagaa gctcatgtaa tgagtgcaag
     4201 agtatttatt cagccgtcgg tttcatttgt taccgattac tttcagtctt cattatcgtt
     4261 gcgcactgtg atggtgcata tatggcaggg agccgtcagc gataacgggt atttttttga
     4321 acctacactt accataaaag ttggagtgcc tgcaataaaa ttagttgcac aggccggttt
     4381 atcacttccc gccaacagtc accatgttag ctttaattac gagccattta ttgtttcagt
     4441 aggattgcag acgcagttca acacagggta taaaaaaaga taaaccggat tgctccggtt
     4501 tatcgtgttg taaatattac catgcaaaaa gcgggaaagc gtgcatcaaa tcgtacaccc
     4561 gtttttttgt tgattcaata acttctggtt tgtcgaagtt cgatatcaca gtatcgatca
     4621 tttccacaat aacatccatg tggttttctt taagccctct tgttgtaatg gcaggagtgc
     4681 ccaggcgtaa ccctgaggtt tggaatggag agcgatggtc gaaaggcacc atatttttat
     4741 ttacagttat ttcggcctga acaagggcat tttctacctg cttcccgtta acatcgggga
     4801 attttgttct gagatcaatc agcatagaat gattatctgt tccaccgctg ataaccttat
     4861 atcctttttt aatcagcgaa tcagccataa ccgctgcatt ctttttcacc tgctgctgat
     4921 ataccctgaa cgatgggtca agagcttcgc caaaagccac cgctttcgaa gcaataacat
     4981 gctcaagcgg tccaccctgc atacccggga atactgctga gttaataatt tcagacattt
     5041 ttttcattac acctttggga gtggtttttc cccaggggtt atcgtaatct ttaccaatca
     5101 aaataatacc accacgcggt ccacgtaatg ttttgtgtgt tgttgaagta acaatgtgag
     5161 catatttaca gggattttca agcaaaccgg cagcaataag tccggcaggg tgcgccatat
     5221 caaccattaa gattgcacca attttatcag ctatctcgcg catacgttta taatcccatt
     5281 cacgactata ggcag
//
LOCUS       DAOOQP010000158         5259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_18909_length_5259_cov_5.031899, whole genome shotgun sequence.
ACCESSION   DAOOQP010000158 DAOOQP010000000
VERSION     DAOOQP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18909_length_5259_cov_5.031899"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLK75_11965"
     CDS             <1..475
                     /locus_tag="PLK75_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40003.1"
                     /translation="HRSWASTGREWDFGKFYNSDGFKRMLGSRVYFGKTNKHLNAETP
                     KAPFGWFASLQFGFTSFKIHNIEMYTFQGDYLNNISVIKRWPELNILFGRQFYLTQHL
                     ALEFYLGTSIYLQYYEKSTIIISGIPEEIGETTIRNYISGSIRPNLCLSLGYHFN"
     gene            complement(810..2261)
                     /locus_tag="PLK75_11970"
     CDS             complement(810..2261)
                     /locus_tag="PLK75_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40004.1"
                     /translation="MQKAIYSSLSVILLNLLCYTLISQESELNRTNHWFFGDSCHVDF
                     SGETPVAYNDGSLYTLEGCSAISDLYGNLLFYTDGMRVYDAQNEQMPNGYGLHGEFSS
                     LNSALIVPKPDDSKIYYIFTTPSYGGAGLNYSHMAYSVVDLSLREGLGDVTLKNIELL
                     PTSAEQLGAVHHKNETDYWVVGHELHNDKFYAFLVTENGVSQEPVVSEVGVYFQTIYE
                     YSVSGFKFSPSGCKAVSTYANLAMFQIFDFDNEHGKLFNPITINIPWPWGACFSPDCR
                     YLYIGMWDTESISNPGEKIFQFDISNTTAESIFASRKLISQDVAGSQAQMQLARDGKI
                     YCSIYQSEYIAVINEPNNYSHACDFVAEGLYLAGSKSLAGITNFVQSFVRPKDQYYAD
                     CPLYETIGENQSHIARLFPNPFSDKTILYFNKSWNNVTLTVFDASGKEVFQANNISGD
                     SYTINRNKLETGLYILQIQHNGIIIDSIKMLVN"
     gene            complement(2499..>5259)
                     /locus_tag="PLK75_11975"
     CDS             complement(2499..>5259)
                     /locus_tag="PLK75_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOY40005.1"
                     /translation="KNYDYCYVEVSTNGTDWTFLSQESYMGEGLYQNAYLFMQYQSEG
                     PCFMESSYPGWQNAAPDNNLWKVETFNLSDFAGEPEVWIRFRLHSDASYLRSGWLLDK
                     IIIYNETCQQPSNLTLINSSGYSALIGWEEEGAATEWELVYGQQGFYPVETGIFVSTT
                     SNQYNITGLSPETHYDVYVRSVCSDDNKSLWTGPFEFISNVPCTAIAGTSLIDADYQS
                     ISLEINPLGLETNWEILYGYSNFIAGEGNLVSVFDNTFMVSGLSDNTEYDFYIRSDCG
                     ANGYSDWVGPFTYATMVTCPPIVGIEVENITYDSFSFLIQAGSTETEWDITYCFQGED
                     PYTEGTLIEGNTNNPVTISDLEPETMYSVYIRANCGAFLGTAEPAGPYQITTLPLCPE
                     VINITASDVQCNSAMISWNAGSEETGWTIEFGEQGFAQGTGTVETLSSPYILLDNLSI
                     NTIYTIYIKANCGGLLGDSDWQHFDFKTACDTISEFPWAEEFAEWEAISQCWDLTGGT
                     NTVMHYNNSAVMANFWSWADGKTAYLTSQLIDKSLLVNPRIEFMWSHKFYSYMSPNDK
                     LELQVSDDDGGTWTNVWVKSGSAFESNDGAGTTSPGSYTTSEPISLANFGDYIKFRFI
                     FTSGYGSDAFIDNVMIYDVLCEPVVDIAIADISYDFAIVEWDNPTGNPVNMYYGLHGF
                     DPASEGNIIHYISANTESYYIFGLLPNTMYDVYIESVCNDVHSELVGPISFTTQAYST
                     ETDILGFSFGEYDADAADIDAVEKLVSLAVIEGTDVTSLAAIFNLSFGAKAYVNGVLQ
                     YSATTENDFSNPLLYLVVAQDEIHQTEWTIDVNILSDIIPETSHSISIYPNPATEYIY
                     LYTTSQMYPDEITLMSSDSRVVSSTRNCTQNTLISLEGTNPGLYLLRIAYSDGNLIWK
                     KFIKI"
BASE COUNT         1582 a         1063 c         1017 g         1597 t
ORIGIN      
        1 gcacaggagt tgggcaagca ctggcagaga atgggatttc ggcaaattct acaattctga
       61 tggttttaaa agaatgcttg gatcgagggt gtattttggg aaaacaaata agcatttaaa
      121 tgctgagacc ccaaaagctc cattcgggtg gtttgcatcg ctgcaatttg ggttcaccag
      181 tttcaagatt cataacattg aaatgtatac atttcaaggg gactacctga acaatataag
      241 tgtaataaaa agatggcctg aattaaatat tttatttggc aggcaatttt atctcaccca
      301 gcatctggca cttgaatttt atttaggtac ttctatctat cttcagtatt acgagaaatc
      361 gaccattatc attagcggaa taccagaaga aattggggaa acgacaattc gaaattacat
      421 atcaggatct ataagaccca atttatgtct aagcctggga taccacttta attgacttgt
      481 agcttatgtt cttaacacac tcgtatgttg ctgccgcata cacagcaata cctttgaaca
      541 attaatcagg gatactcaat acgcaacatt tgactttcat ggagcaagcg tggatgcctg
      601 aagcctaccg gaggatgtcc cttaacaata gatttttatt agattatagt ttgagttaaa
      661 cagtaagcgt ggacgcttac tgcaatgatg gggtatcttt gatgcctcaa caatggtgcc
      721 taggttatat ccggcagtca cttttgttgc attttcggca tatcggttaa tgctatggct
      781 tactaattac tgtgggtatt tgaaaaaggt tagttgacta gcattttaat agagtcaatt
      841 ataattccat tgtgctgtat ttgtaaaata tacaatcctg tttcaagctt gttgcgattg
      901 atggtataac tgtcgcctga aatattgttt gcctgaaaca cctcctttcc ggatgcatcg
      961 aaaacggtca gggttacatt attccatgat ttgttaaaat aaagaattgt tttgtcggaa
     1021 aaagggtttg gaaacaatcg ggcaatatgg gattgatttt ctccaatggt ttcgtacaac
     1081 ggacaatcgg cataatactg atctttcggt cttacaaaac tttgtacaaa attggttatt
     1141 cctgcaagag atttgctacc agccagatac aacccttctg ccacaaaatc acatgcgtgg
     1201 gaataattgt tgggctcatt aatcaccgct atatattcgc tctgatatat tgaacagtaa
     1261 attttaccat cacgtgccag ttgcatctgt gcctgcgaac ccgctacatc ttgtgaaatc
     1321 agttttcggg aagcaaaaat gctttcagca gttgtatttg atatatcgaa ctgaaatatt
     1381 ttttctccgg gattactaat actttctgtg tcccacatcc caatatataa atacctgcag
     1441 tctggtgaaa aacacgcacc ccatggccat ggaatattta ttgtaatagg gttaaatagt
     1501 ttgccatgct cattatcgaa atcgaaaatt tgaaacatag ccagatttgc ataggtagaa
     1561 actgccttac accctgatgg agaaaattta aagccactta ctgagtactc atatattgtt
     1621 tgaaaataga ctcccacttc agaaacaacc ggttcctgag aaacaccgtt ttcggtaaca
     1681 agaaatgcgt agaacttatc attgtgcaac tcgtgaccta ctacccagta atcggtttca
     1741 tttttatgat gtacggcccc caattgttcg gccgatgttg gcaacagttc aatgttcttt
     1801 aaagtaacat ctcctagtcc ttcccgcagc gacaaatcta caaccgaata agccatatga
     1861 gaataattca atcctgcgcc tccatacgaa ggagtggtaa aaatgtaata tattttgctg
     1921 tcatccggtt taggaacaat taatgctgaa tttaaagacg agaactctcc gtgtaagcca
     1981 tatccgttag gcatttgctc gttttgcgca tcgtaaaccc gcataccatc agtataaaaa
     2041 agcaggtttc cgtataaatc agaaatagcc gaacaacctt ctaatgtata taacgaacca
     2101 tcgttataag caacgggtgt ttcgcccgaa aaatcgacat gacaactatc cccgaaaaac
     2161 caatggtttg tacggtttaa ttccgattcc tgagaaatta gggtataaca aagcaaattc
     2221 agaagtatta cagataaaga gctataaatc gctttttgca tgattattta aattttaaca
     2281 agatatataa ctaaatatta acaaatttaa atataaaatg tattattaat atcattgtcg
     2341 acaaaaattt tataagtact aatatttatt tattttcaag ttaccaagtt atgtggcaat
     2401 gagaaatgca atattttacg aatcaacttg gtaaattatg ataatattat gtttgacctg
     2461 aagaaaaaat aaatctgttt ttgggtgttt tctcaggatt atattttaat gaattttttc
     2521 caaattaagt ttccatcaga atacgctatt ctgagcaaat ataagcctgg atttgttccc
     2581 tccagagaaa ttagcgtgtt ttgtgtgcaa ttgcgggtag aggaaacgac cctgctatcg
     2641 ctgctcatta gtgttatttc atccggatac atttgacttg ttgtgtacaa ataaatatat
     2701 tctgttgccg gattggggta gatgctaatg ctatgtgagg tttcaggtat tatatccgac
     2761 aaaatattca catcaatggt ccattcggtt tgatggattt cgtcctgtgc aacgaccaaa
     2821 tatagcaaag ggttgctgaa atcgttttcg gttgttgccg aatattgtaa tacgccattc
     2881 acgtaggctt tggcaccgaa cgaaagattg aatatcgctg ccagactcgt aacatcggtg
     2941 ccttcaatca ctgctaaaga aaccaacttt tccacagcat caatatctgc agcgtcggca
     3001 tcatattccc caaacgaaaa ccccagaatg tcggtttcgg tgctgtaggc ttgcgtagta
     3061 aagcttattg gccccacaag ttcggagtgc acatcattgc aaaccgactc aatgtaaaca
     3121 tcgtacattg tattgggcaa taatccgaaa atatagtatg attctgtatt tgcactgata
     3181 taatgaatga tatttccttc tgacgcaggg tcaaatccat gaaggccgta atacatgttt
     3241 accggattgc ctgtggggtt atcccattca acaattgcaa aatcgtagga aatatcggca
     3301 atggcaatat ccacaacggg ctcacagaga acatcgtaaa tcattacatt gtcgataaag
     3361 gcgtcagacc cgtatcctga tgtgaagatg aacctgaatt taatataatc gccgaaatta
     3421 gccaaagaaa tcggttctga tgttgtatag gatccagggc ttgtggttcc ggctccatca
     3481 ttcgattcga atgcactccc cgactttacc cacacgtttg tccatgttcc tccatcatca
     3541 tcgctgacct gaagttcaag tttatcgttg ggagacatat aggaataaaa cttatgagac
     3601 cacataaatt caatccgagg gtttaccagc aaagatttat caatcaactg acttgttaaa
     3661 tatgcggttt tcccgtctgc ccacgaccag aaattagcca ttaccgcgct gttattgtaa
     3721 tgcataaccg tattggtgcc accggtaagg tcccagcact gtgaaattgc ttcccattca
     3781 gcgaattctt cagcccatgg gaattccgaa attgtatcgc atgctgtttt aaaatcgaaa
     3841 tgctgccaat cggaatcgcc aagtaaaccg ccgcaatttg cttttatgta aatagtgtaa
     3901 atggtgttta tcgacaggtt gtcaagcagt atataaggag atgatagggt ttcaacggta
     3961 cctgttccct gagcaaagcc ctgttcgcca aattcaattg tccatcctgt ttcctctgaa
     4021 cctgcattcc agcttatcat ggcagaattg cattgcacat ctgaagccgt aatattgata
     4081 acttccggac acaaaggcaa ggtggtaatt tgatatggtc ctgccggctc agctgtgcca
     4141 agaaacgctc cgcagtttgc ccttatatat accgaataca ttgtttcagg ttcaaggtct
     4201 gaaattgtaa cagggttatt ggtattgcct tcaatcaggg tcccttctgt ataggggtct
     4261 tctccctgaa aacagtacgt aatatcccat tcggtttctg ttgaacctgc ttgtattaaa
     4321 aaactaaaac tatcgtaggt aatattctca acttcgatac caacaatagg tggacacgtt
     4381 accattgttg catatgtaaa gggacctacc caatccgaat acccgttggc gccgcaatca
     4441 gacctgatat agaagtcata ttcggtatta tcgcttaatc cagataccat gaatgtatta
     4501 tcaaaaaccg aaaccaggtt tccttctcct gcaataaaat tggaataacc atacaatatt
     4561 tcccaattcg tttccaatcc gagggggttg atttctaagc taattgattg ataatctgca
     4621 tcaattaagc ttgtacctgc gatggctgta catggcacat tactgataaa ctcaaacgga
     4681 ccggtccaca ggcttttatt gtcgtcagaa caaaccgaac ggacatatac atcataatgg
     4741 gtttccggac tgaggcctgt aatattgtat tggttgctgg tggtagaaac aaagattccg
     4801 gtttcaacag ggtagaatcc ctgctgaccg taaaccaatt cccattctgt agcggctccc
     4861 tcttcttccc agccaattaa ggctgaatat ccggatgaat taattaatgt aagattagag
     4921 ggctgctgac aggtttcgtt ataaattata attttgtcta ataaccaccc gcttcttaga
     4981 taggatgcat ctgaatggag ccggaacctg atccatactt caggttcgcc tgcaaaatcg
     5041 ctcagattga aggtttctac tttccataaa ttattatcag gtgcagcatt ttgccaaccg
     5101 ggatatgatg attccatgaa acatggacct tccgactgat actgcatgaa aagatatgca
     5161 ttctgataaa gcccttcacc catatagctt tcctgtgaca ggaacgtcca gtcggttccg
     5221 ttggttgata cctcaacata gcaataatcg tagtttttc
//
LOCUS       DAOOQP010000159         5254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_18933_length_5254_cov_6.357376, whole genome shotgun sequence.
ACCESSION   DAOOQP010000159 DAOOQP010000000
VERSION     DAOOQP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18933_length_5254_cov_6.357376"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLK75_11980"
     CDS             <1..969
                     /locus_tag="PLK75_11980"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOY40006.1"
                     /translation="IGLFRTEHMFYGKGADEPLFILRKMILASTDAERKAAIDELFPF
                     VKRDMKGTLEVMDGLPVTFRLLDPPLHEFAPHTPEKQAEIAKVIGISIEEVVKRVDKL
                     HETNPMMGHRGVRLGVTYPAITEMQFRALFEATAELLKAGKKPIPEIMVPVTCDVNEL
                     NVSKVIFDKVKKETEAKFGISEIKCLYGTMIEIPRACLLANNMAKTAEFFSFGTNDLT
                     QMTFGFSRDDVSGFMHDYLDNKIIPADPFQTIDQDGVGQLIEMAVVKGRDTRNNLKVG
                     ICGEQGGDPASVEFCFKNNLNYVSCSPFRVPIARLAAAQATIKYGK"
     gene            complement(1204..1938)
                     /locus_tag="PLK75_11985"
     CDS             complement(1204..1938)
                     /locus_tag="PLK75_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40007.1"
                     /translation="MKQKIIISILLALLMSGCRSIYYEATTPNIPVFDEGEIINGGVE
                     LSNHGFNSNMNFRILKYGVFQANASYCDWNFFLGGANSFYSFGFGGVYQSDKMHVSLV
                     TGYGKGWSHFSEGIMDHEFKGNGVFDQVYFQPALAFGNKYFKVGLAAKTALMQYKIIS
                     TTWGGPDNPGGLLLNHNYKCLSIEPVLFCRVNMNSFFYGTFYLGRNFFFSEIPFYHNM
                     SKTEVYIGFGVGLKMSKQMFHSNKSE"
     gene            complement(1940..2542)
                     /locus_tag="PLK75_11990"
     CDS             complement(1940..2542)
                     /locus_tag="PLK75_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40008.1"
                     /translation="MPAIEPNTKHRGAELLGISNDSAIPKRNAIKLQLSLSNFTLGYE
                     RVLTNKINFEFKCGYIWVPSLFFSDNYPGDNSSTNGALVKIGIKCVVKPKIFRKGAIS
                     QPQLNGLYLKPEIFNKLRMTLYSDNLETNYFENNGGLQLLFGYEFFVTKSLLIDLYAG
                     WGLNYRYVSSVNNSIIRLAPFKSCIYPNISLGICVGFSFK"
     gene            2781..3170
                     /locus_tag="PLK75_11995"
     CDS             2781..3170
                     /locus_tag="PLK75_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40009.1"
                     /translation="MKKSILLFFILTFGFSFFIYADQLQWLSKEQAEQAVQYLHDAKV
                     KQAVLWCACCDNDPAVKIKIKKAEIRYTGTGDYYEVIITGKLADGTPFSDAVDLAYFH
                     IKSGDQAYCFGAVLNFDCDPCTGPFPW"
     gene            3219..4541
                     /locus_tag="PLK75_12000"
     CDS             3219..4541
                     /locus_tag="PLK75_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40010.1"
                     /translation="MSIFNKLFGKTETQTDTLAAYTAGRYTDINKSKKQTEAWEQAKK
                     EFGEKNYIESYYNLFMYLNDPAKNSVTVNRSDNSIEFTLEQGSKMIKGIADNERFVAE
                     TRIASFETLNIAFLRKLMNMNYALQYCRFAIKDNDIYLKLTSKTLDASPNKLYYSLKE
                     LALKADKLDDALINEFAMLKPIDVNHIQNTAASVRETRYKYLKQWIETELEKASKLNE
                     DHLAGGISYLLLALLYRIDYLLVPQGRVLNDIEKINGIFYNPNENISPVERNRSMTEE
                     FKKLAAVDKEILLKEFYDIKATFGYVSATSHKTFYEFVLEQFKNTGWYYDNKHPDVVT
                     AIYEYIMGYAMFSFGLYPATIELIQLINNVLHNEFFKEMGLTNEYYSKTTGKFNIPLI
                     EKEIQRIMAKHRSDYPKLSFTLNKLGYTNMNEFLYTALNEITYLNFSK"
     gene            4547..>5254
                     /locus_tag="PLK75_12005"
     CDS             4547..>5254
                     /locus_tag="PLK75_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOY40011.1"
                     /translation="MANIFDHFKNVIIQIATPFGSGTGFYNHEHQVFITNYHVIEGCN
                     EVIISGKNFKKTTTKVLYFDPYFDLAFLEVPEGIAVETAPISEHQLTEGNTIFAIGHP
                     YGLKYTATKGIVSKAQRYYNEIAYIQIDAAINSGNSGGPLVEESGEIAGVNTFIIADG
                     ESLGFSLPAKYVRQALDEFEALNRVASTRCKSCKKVLSAGMIDSGYCSNCGATINLEI
                     MNPKPYIPAGAALNIEKM"
BASE COUNT         1721 a         1090 c         1006 g         1437 t
ORIGIN      
        1 atcggactgt tccgtaccga gcatatgttc tatggcaaag gagctgatga gcctctgttc
       61 attctccgca aaatgatact tgcttcaacc gacgcagaac gtaaagctgc tatcgatgaa
      121 ttgttcccgt ttgtaaaacg cgatatgaaa ggcactctcg aagtaatgga cgggctgccg
      181 gtaactttca gattgctcga cccgccactg catgagtttg ctccacatac cccagaaaaa
      241 caggctgaga ttgcaaaagt tattggcatc agcatcgaag aagtggttaa acgagttgat
      301 aaactccacg aaaccaaccc gatgatgggt caccgtggtg ttcgccttgg cgttacctac
      361 cctgcaatca ccgaaatgca gttcagggct ctcttcgaag ctacagcaga attgctgaaa
      421 gcaggtaaaa aaccgatacc cgaaatcatg gtacctgtta cctgcgatgt taacgaactc
      481 aatgtttcga aagtgatttt cgacaaggtt aaaaaagaaa ccgaagccaa attcggcata
      541 agcgaaatca aatgcctgta cggaaccatg atagaaattc cgcgcgcttg cctactggca
      601 aacaatatgg caaaaacagc cgagtttttc tcattcggca ccaacgacct tacccagatg
      661 accttcggtt tcagccgtga cgacgtaagt ggattcatgc acgattacct cgataataaa
      721 ataatccctg ccgacccatt ccagaccatt gaccaggatg gcgttggaca gcttatcgaa
      781 atggcagtgg taaaaggacg tgacacacgt aataacctga aagtgggaat ttgcggcgaa
      841 caaggcggcg atcctgcttc ggtggaattc tgctttaaaa acaatctgaa ttacgtaagc
      901 tgctcgccct tcagggttcc tattgcccgt ttggcagctg ctcaggcaac tattaaatac
      961 ggtaaataac attttctgaa tattgaaaag ccggtgtaac agccggcttt tttttactat
     1021 taattatcct gctttactgg cttttacaag tgcgattgta aataccacgg gaattattca
     1081 gtatttacat attaaccacc aacttgaact tgctgaaatt acatcagtta agcaaataag
     1141 aattttttgc tgttggtgac taagggaatg cagttgtaat tagtgaattt tgcaaataag
     1201 aatctactca ctcttatttg agtggaacat ttgctttgac attttcaagc ctacaccaaa
     1261 ccctatatac acttcagttt tagacatgtt gtgataaaac gggatttctg aaaagaaaaa
     1321 attccgcccc agatagaaag tgccgtagaa aaacgaattc atatttaccc tgcaaaacaa
     1381 aacaggttct attgaaagac acttgtaatt gtggtttagc aaaagaccgc ccggattatc
     1441 aggaccaccc caggtggttg aaatgatttt atactgcatt aaggctgttt ttgcagctaa
     1501 tcctacttta aaatatttgt ttccaaaagc taaagcaggt tgaaaatata cctgatcgaa
     1561 aacgccgttt cctttaaact catgatccat aattccttct gaaaagtgcg accaaccttt
     1621 accataacct gttaccaaac taacatgcat tttatctgat tgatatactc ctccaaaacc
     1681 aaaagaatag aatgaattcg ccccaccaag aaaaaagttc caatcgcaat aactcgcatt
     1741 agcttgaaaa acaccatatt taagaattct gaaattcatg ttgctattga acccatgatt
     1801 acttaattcg acgcccccat tgataatttc accttcatca aaaacgggta tattaggagt
     1861 ggtagcttca taatagatac ttcgacaccc cgacatcagt aaggcaagta gtattgaaat
     1921 aataattttc tgtttcatgc tatttaaaac tgaatccaac acatatacca agacttatat
     1981 ttggatatat acatgacttg aaaggagcaa ggcggattat tgaattgtta actgaactta
     2041 cataccgata attcaaaccc cagcctgcgt acaaatcgat caacaacgat ttagtaacga
     2101 aaaactcgta accaaacaat aattgcagcc ctccattgtt ctcgaagtaa ttagtttcca
     2161 ggttgtcgga atacagagtc atccttaatt tattaaaaat ctctggtttt agatataatc
     2221 cgtttaattg aggttggctt attgcgcctt ttcggaaaat tttgggttta actacacatt
     2281 ttattcctat tttcactaat gcgccattgg ttgaactatt gtcaccagga taattatccg
     2341 aaaaaaacag tgaaggaacc caaatataac cacatttgaa ttcgaaatta attttattgg
     2401 tcaacacccg ctcgtagccc aaagtaaaat tgctcaaact gagttgcagc tttatggcat
     2461 tccgttttgg aattgcacta tcattactaa ttcctaataa ttcggcacct ctatgctttg
     2521 tatttggttc tattgccggc acacaaaaac attcaccaca aaaaattgta ataaaaataa
     2581 gctgtaggaa tattttattt atcaaagcga atcgtatatg ttaatgcaaa tatactgaac
     2641 attaatgata gaacaactta taacacatac aaaggacggc aggctaagtc atagtcagat
     2701 ttctccaaaa taatagtagc caattatgaa aacagaaaaa gtttttataa tattgaattg
     2761 taatattcat taccgatatc atgaagaaat cgattctgct attttttata ctgactttcg
     2821 gattcagttt ttttatttat gccgatcagt tgcaatggtt gtcgaaagag caggctgagc
     2881 aggctgtaca atacctgcac gatgcaaaag tgaagcaggc tgttctttgg tgtgcctgtt
     2941 gcgacaacga ccctgccgta aaaataaaaa ttaaaaaagc cgaaatccgc tacaccggta
     3001 ccggcgacta ttatgaagtg ataatcaccg gaaaacttgc cgacggaact cctttttcgg
     3061 atgcagttga ccttgcctat tttcatatta aatcaggcga ccaggcatat tgcttcgggg
     3121 cagttctgaa tttcgactgc gacccatgta ccggcccatt tccctggtaa tttcagagtt
     3181 aaatacatac aaaccgataa agaacttcaa acaaaagcat gtcaatattt aataaattat
     3241 tcggaaaaac cgaaacacag actgatacct tagcagcata cactgcaggc aggtacaccg
     3301 acattaacaa gtcgaagaaa cagacagaag catgggaaca ggcaaaaaaa gagtttggcg
     3361 aaaagaacta tattgaatcg tactacaacc tgttcatgta tctcaacgat cctgcaaaaa
     3421 acagtgttac tgtgaaccga agcgacaaca gcattgagtt tacactggag caagggtcga
     3481 agatgataaa aggaattgcc gataacgaaa ggtttgttgc agaaacgcgt attgcaagct
     3541 tcgaaacact gaatattgca tttctgcgca aactgatgaa tatgaattat gccttgcaat
     3601 actgcaggtt tgcaataaaa gacaatgata tttacctgaa actgaccagt aaaacgctgg
     3661 atgcatctcc caataaattg tattattcac tgaaagagct tgccctgaaa gccgataaac
     3721 tcgatgatgc gcttatcaat gaatttgcta tgctgaagcc catcgatgtg aatcatattc
     3781 agaatactgc tgcatcggtg agagaaaccc gatataagta cctgaagcaa tggattgaaa
     3841 ctgagcttga aaaagcttca aaacttaatg aagaccatct ggcaggaggt atttcgtacc
     3901 tgttgcttgc gctgctttac cgcatcgact acctactggt gcctcagggc agggtgctca
     3961 acgatattga aaaaatcaac ggtatttttt acaatccgaa cgagaacatc agtccggtgg
     4021 aacgaaaccg ttcgatgacc gaagagttta aaaaactggc tgcagtcgat aaagaaatcc
     4081 ttcttaaaga attttacgat attaaagcca ccttcgggta tgtttcggct acctcgcata
     4141 aaacatttta cgagtttgtt ctggaacaat ttaaaaatac cggctggtat tacgacaaca
     4201 aacacccaga tgtggtaacc gcaatatatg agtacatcat gggatatgcc atgttcagtt
     4261 tcggcctcta ccctgccacg attgaactta ttcagctgat aaacaatgtg ctgcacaacg
     4321 aatttttcaa agaaatgggc ttaactaatg aatattacag caaaacaacc ggaaaattca
     4381 acattcccct aatcgaaaaa gagattcaac gaattatggc aaagcaccga agcgattacc
     4441 ccaaactcag ttttacgctg aataaactgg gatacaccaa tatgaacgag tttttataca
     4501 cagcactgaa tgaaattacc tatttaaatt tttcaaagta acagctatgg caaacatatt
     4561 cgatcatttt aaaaatgtaa ttatacaaat tgccacccca tttggcagtg gtaccggttt
     4621 ctacaatcat gaacatcagg tttttataac aaattatcat gtgattgaag gctgcaacga
     4681 ggtaataatc agcggaaaga attttaaaaa gaccaccaca aaggtcttgt attttgatcc
     4741 gtattttgac cttgcgtttc tagaggtacc cgaagggatt gctgttgaaa ctgcacctat
     4801 ttcggagcat cagcttaccg aaggcaatac tatttttgcc attggccacc cttacggact
     4861 gaaatacact gctaccaaag gaattgtttc gaaagcccag cggtattaca acgaaatagc
     4921 ctacattcag attgatgcag ccatcaattc ggggaatagc ggtggtccgc tggtggaaga
     4981 aagcggagaa attgccggag taaatacatt tattattgcc gatggcgaaa gtctcggatt
     5041 ctcgcttccg gccaaatatg tgcgtcaggc tcttgatgaa ttcgaagcct tgaacagggt
     5101 tgcttcaaca cgctgtaaat cgtgcaagaa agtactgtcg gccggtatga tcgactcggg
     5161 ctattgttcc aactgcggag ccacaattaa tttggagata atgaatccca agccatatat
     5221 ccccgccggt gcagcactga atatcgaaaa aatg
//
LOCUS       DAOOQP010000160         5215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_19129_length_5215_cov_4.495543, whole genome shotgun sequence.
ACCESSION   DAOOQP010000160 DAOOQP010000000
VERSION     DAOOQP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19129_length_5215_cov_4.495543"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PLK75_12010"
     CDS             complement(<1..1821)
                     /locus_tag="PLK75_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40012.1"
                     /translation="MRGCLTILSVLIFINSSVVVAQNLSDSVDCRNTINFGWCNVVGT
                     DTSDAVQGVCSDAEGNVYVTGYFNHTMQIGETILTSQGAKDIFIVKYSADGDFIWALS
                     AGGIDDDYGKAIACNSTGDIFVTGMFTGSALFDGEEMFAYALEDAFIVSYNGNGDMLW
                     FQPAGGWHADSGSDIKVDARDNVLVTGTFNEAMGIGSTTLIGNGQDDCFAAKFRSNGS
                     FLWAKKIGSSVNDESTSIAFDHDDNVFVTGVFTGTLETEAETLTASGTHDGFIAAFNA
                     VGEMQWIKQVGSDANNDRVNDVTADGDGNVIVAGFFDHDDQPVFVRKYDAQGTEIWTK
                     LTGGDADASAVSITSDLSNNLFVTGWFSGTAVFGNSMFGAMGNKDAFFIKISHDSEIL
                     MVKTMGSGNNDEGRDVCLDYLQNIIVGGLYSSNIYIEGSPYTSEGLTDGFVIKYNRLI
                     SMGNFTFSGIGCDDNNLCAQINMLQGAEPYSYYWSTGDATAQVCGLSPADYTVTVVDQ
                     NNCYIDTVITVLPSIYPEPYLPEQMNICPFDTVNLDAGEGMQTYLWSTGANTQFIEVS
                     GPGAFSVTVTNEYECELVQNITVSESAVIELFGIDTVFTCF"
     gene            complement(1824..2828)
                     /locus_tag="PLK75_12015"
     CDS             complement(1824..2828)
                     /locus_tag="PLK75_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOY40013.1"
                     /translation="MRRIAFIVFAFLVVHTVHSQNMYVHEQYVFERFLLNPAFTGLRE
                     GSSIRLTHRQQWTGIAEGPKTSFLSFNSRMGSQPIGIGGYIANDLNGPEQKIGFQLTF
                     AYHLILTAKRFDQTVLSMAMSFNGQYHILDETAFNPDIYDPLITYTRQSSFLPDFNAG
                     IMLSRNKYMFGVSVDHLIPSYDKLYNREIESLSPMYTHFHGAYVFGLSRGIDLKTFVN
                     VRTDFKAHNQMEVMVRASYAYGKKIQSAYMRNPNEFYVGFCYRHTLDYLNSAPLSLQP
                     TFGVKIGAFTFAYLYDMGLTAIQLYNYGSHQLSIGLFLVGDKTLNVGRNRVPIINTDF
                     "
     gene            complement(2830..3513)
                     /locus_tag="PLK75_12020"
     CDS             complement(2830..3513)
                     /locus_tag="PLK75_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOY40014.1"
                     /translation="MTLTDRIERFVAQLPDNVALVAVSKYQPVEIIQEAYNAGLRYFG
                     ENKVQEIVEKHHALPSDIQWHFIGHLQTNKVRKIASFITCIQSVDSVRLMEEIQVQAK
                     KNNRVIDCMIQFHIAREEAKYGFTFESFTDFLHSGALIGFSNLNIVGVMGMATFTEDM
                     SQIRSEFKKLAAYYNELKSVYFSNKQDFKEISMGMSDDWIIAVEEGTTIIRIGTAIFG
                     QRQKTNTLV"
     gene            complement(3625..4098)
                     /locus_tag="PLK75_12025"
     CDS             complement(3625..4098)
                     /locus_tag="PLK75_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY40015.1"
                     /translation="MKARNASYNDLETLTGFQIQMAYETEGIKLNEEVVRKGITAVIH
                     DSTRGKYIVVENEKKIIACCMITPEWSDWRCCWVLWLQSVYVEPAYRSTGVFKFLFNH
                     IKKQVINSSEYAGIRLYVDKRNQSAAAIYTKLNMNSDHYYMFEWLKNENDSVVQM"
     gene            complement(4085..5053)
                     /locus_tag="PLK75_12030"
     CDS             complement(4085..5053)
                     /locus_tag="PLK75_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOY40016.1"
                     /translation="MKINNHIQFILKLSIAIMAYLYVIYVIINAPDLNSVFTVPSGFG
                     RFQALLILLVFLLMILNWSIEGIKWSYLLKSCYNLSFKRSLRSVLAGTTAGMATPNRL
                     GEFAGRILFIPRENRANAALLSITGSFTQLFVTLFFGLAAFINLIIYVPASRGVFAVN
                     DWIIIAVSVACMALMLWFYGNTKLLARVVSKIGFLKNKLNFSKTDLVPGARKKWLVLL
                     LSAFRYVIFVHQFYIMIVLFGIDTSYVETMQAVMCIYLLMAIIPVISAGEPGVRGTVS
                     IVLFSLFTDQPATVFSASISLWLINLAIPALIGSFLLFQNKKNEST"
BASE COUNT         1589 a         1160 c          937 g         1529 t
ORIGIN      
        1 aaagcaagta aacactgtgt cgataccaaa cagttcaatt actgccgatt ctgatacagt
       61 tatattctga acaagttcac attcatattc gttggttacc gttaccgaaa aagctcccgg
      121 tccacttact tcgatgaatt gtgtgttggc accggtgctc cataaatatg tttgcattcc
      181 ctcacctgca tcaagattca ccgtatcgaa cggacaaata ttcatctgtt caggaaggta
      241 tggttcagga tatatcgagg gtaacactgt aatcacagta tcgatatagc aattattctg
      301 atccactaca gtaactgtgt agtcggcagg agataatccg caaacctgag ctgtagcatc
      361 gcctgttgac caataataac tataaggttc tgccccttga agcatgttta tctgtgcaca
      421 caagttatta tcatcacatc cgattcctga gaatgtaaaa ttgcccatgc ttatcaatct
      481 gttatacttg attacaaagc catcggtaag accctcactt gtgtatgggg agccttcgat
      541 gtatatgtta cttgagtaaa ggcctccaac aattatattc tgaagataat caagacaaac
      601 atccctgcct tcgtcattat tgccggagcc catagtttta accatgagta tttcggaatc
      661 gtgtgatatt ttaataaaaa aggcgtcttt gtttcccatg gctccaaaca tagaattacc
      721 gaaaaccgca gttcctgaga accatccggt aacaaaaagg ttattactga ggtcagaggt
      781 tattgaaact gccgatgcat cagcatcccc accggtaagt tttgtccata tttcagtacc
      841 ttgtgcatcg tattttctta caaatacggg ttgatcgtcg tggtcgaaaa agcctgcaac
      901 aataacatta ccatcgccat ctgccgtaac atcatttacc ctatcgttat tagcgtcgga
      961 gcccacttgc ttaatccact gcatttctcc aacagcatta aatgcggcta taaaaccgtc
     1021 gtgagttcct gatgcagtca atgtttctgc ttctgtttca agggtgccag tgaacacacc
     1081 tgttacaaat acgttatcat cgtggtcgaa agcgatagat gtggattcgt cattcacgga
     1141 ggagccaatt ttctttgccc aaaggaaact tccgttacta cgaaacttag cagcaaagca
     1201 atcgtcctga ccatttccta taagcgtagt agacccaatc cccattgctt cgttgaatgt
     1261 tccggttacc aaaacgttat cacgggcatc taccttaata tcggacccgg aatcggcgtg
     1321 ccatcctccg gcaggctgaa accataacat atcaccatta ccgttgtatg aaacaataaa
     1381 ggcatcctct aaagcatatg caaacatttc ttctccatca aacaaagccg atccggtaaa
     1441 catacctgta acaaaaatat cacctgtgct gttgcaggca atggctttgc cataatcatc
     1501 atcgattccg ccggcactta gtgcccaaat aaaatcccca tccgcactgt attttacgat
     1561 aaatatatct ttggcgccct gcgacgttaa tattgtctcg cctatttgca tcgtatgatt
     1621 aaaatagccc gtaacataaa cattaccttc agcatcggag caaaccccct gaactgcgtc
     1681 cgatgtgtcg gtgcccacga cattgcacca tccaaaattt attgtattac ggcaatcgac
     1741 tgaatcggaa agattctgtg caacaactac tgatgaattg ataaaaataa gtacggataa
     1801 tatagttagg cagcctctca tacttaaaaa tcagtgttaa taattggtac gcggtttcga
     1861 cctacattca gggttttgtc tccaaccagg aatagcccga tagaaagctg gtgagaacca
     1921 taattataca attgaatcgc agtaagaccc atatcgtaaa gataggcaaa agtaaatgca
     1981 ccaattttaa cgccaaatgt aggttgtaaa ctcaacggtg cagaattcag gtagtcaagc
     2041 gtatgtctgt aacaaaaacc cacataaaat tcgtttgggt ttcgcatata ggccgactga
     2101 attttctttc cataagcata cgaagccctt accattactt ccatctgatt atgcgcttta
     2161 aaatcggttc gcacattaac aaatgttttc aggtcaattc cacgcgacaa accaaaaaca
     2221 taagcaccat gaaagtgagt gtacattgga ctaaggcttt caatttcgcg gttatagagt
     2281 ttgtcataag aagggattaa atgatcgaca ctaactccga acatgtattt atttcgggaa
     2341 agcattattc ctgcgttaaa atcgggtaaa aagctgcttt gccgcgtata ggttataagt
     2401 gggtcataaa tatcgggatt aaaagctgtt tcatccagaa tatgatactg tccattgaac
     2461 gacatagcca ttgacaatac ggtttgatcg aatcgttttg cagtaagtat caggtgataa
     2521 gcaaatgtga gttgaaaacc tattttctgt tccggaccat tcaaatcatt tgcaatatac
     2581 cctcctatcc caattggttg gctgcccata cggctgttga agctgagaaa agatgtcttg
     2641 ggcccttctg caatacctgt ccattgctga cggtgtgtca gtcgaatgct ggatccttcc
     2701 ctcaacccgg tgaaagcggg atttaacagg aatcgttcga aaacgtattg ctcatgaaca
     2761 tacatatttt gcgaatgaac agtatgtacc actaaaaatg caaatactat gaaggctatt
     2821 ctcctcatat taaaccaacg tatttgtttt ttgtctttga ccaaaaattg ccgttcctat
     2881 acgtatgatt gttgtaccct cttcaactgc aattatccaa tcgtcggaca tacccatact
     2941 tatttcttta aaatcctgtt tgttactaaa gtaaactgat ttcaactcat tatagtatgc
     3001 agccagtttt ttaaattcac ttcttatttg actcatgtct tcggtaaaag ttgccatgcc
     3061 cataacacca actatattta gattcgaaaa gcctattagt gctccagaat gcaaaaagtc
     3121 ggtgaacgat tcgaaagtaa agccatattt cgcttcctca cgggctatat ggaattggat
     3181 catgcaatca atcaccctgt tgtttttttt agcctgaact tgaatttcct ccatgagccg
     3241 aacactgtca acgctctgga tacaagtaat aaatgaggca atttttctta ctttattggt
     3301 ttgcaaatgg ccgataaaat gccattgtat atcagaaggt aaggcgtgat gtttttcaac
     3361 tatttcctga actttgtttt ccccaaaata acgaaggccc gcattgtatg cttcttgtat
     3421 aatttcgacc ggctgatatt tcgacacagc aaccagcgca acattatcgg gaagctgtgc
     3481 aacaaatcgt tctattctat ccgtaagcgt catcgcagtc agtgtttacc ccacaaattt
     3541 aagcaaatta atgcgtgttt tacattggtc tgataaaact ctcaacatca atcaatcctg
     3601 aaaattagat attttttcta ttgcttacat ctgtacaact gagtcatttt cgttttttaa
     3661 ccattcaaac atatagtaat gatctgaatt catgttcagt tttgtataaa tggcagctgc
     3721 cgattgattt cttttatcca catacaggcg tatcccggca tattcactgc tattaatcac
     3781 ctgtttttta atatgattaa acagaaattt aaacacaccg gtgctacgat atgccggttc
     3841 aacatataca ctttgcagcc acaacaccca acagcatcgc caatcgctcc attcgggtgt
     3901 aatcatacag catgctataa tttttttctc attctcgacg acaatatatt ttcctcttgt
     3961 actatcatga ataaccgctg taataccctt acgcactact tcttcgttga gcttaatacc
     4021 ctctgtttca taagccattt gtatctgaaa cccggttaga gtctccaaat cgttgtatga
     4081 tgcattacgt gctttcattt tttttatttt ggaataacag gaatgaccca atcagggccg
     4141 gtattgcaag gtttatcagc cacaaagaga ttgatgctga aaacactgtg gcaggttgat
     4201 cggtaaataa agagaacagc acaatgctaa ccgttccacg cactcctggt tcaccagccg
     4261 agattaccgg gataatagcc attagtagat aaatacacat tacggcctgc atggtttcca
     4321 cataggaagt atctatcccg aataatacaa tcattatgta aaactgatga acaaagatta
     4381 catacctaaa agcactcagt aataatacaa gccatttttt tcgggctccg ggcactaaat
     4441 ctgttttgct gaaattcagt ttatttttca aaaagccaat tttcgaaacc actctggcaa
     4501 gcagttttgt atttccgtag aaccacagca tcagtgccat acacgcaaca gaaactgcaa
     4561 taataatcca atcgtttaca gcgaaaactc cacggctggc cggaacataa ataatcaggt
     4621 tgataaatgc tgccagcccg aaaaacaggg ttacaaacaa ctgtgtaaaa ctacccgtta
     4681 tacttagtaa ggctgcgttt gcacggtttt cccgcggaat aaaaagtatt cttccggcaa
     4741 actctcccaa acggtttggc gttgccattc ctgctgtggt tcctgctaaa acggacctaa
     4801 gagatctttt gaaagacaag ttatagcatg atttcaacag atacgaccat tttatgcctt
     4861 cgattgacca atttagtatc atcagcaaaa ataccaacaa tatcaaaagc gcctgaaatc
     4921 taccaaatcc tgaaggaaca gtgaagacac tattaagatc gggagcgttg ataataacat
     4981 aaataacata cagatatgcc attatcgcaa tactcagttt cagaatgaac tgtatgtgat
     5041 tattgatttt catcaggggg tttacgcatt ttcggaagaa gtactaccgg cggggatctt
     5101 attgatcaaa ggcagtgcta taagcgatat caatccggca cccaacaaca tcagtgtttg
     5161 tagcccgtga actacaaaag caaaaacagc cgcatcctct ttaacaatcc cgaaa
//
LOCUS       DAOOQP010000161         5182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_19277_length_5182_cov_5.695533, whole genome shotgun sequence.
ACCESSION   DAOOQP010000161 DAOOQP010000000
VERSION     DAOOQP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19277_length_5182_cov_5.695533"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1403)
                     /locus_tag="PLK75_12035"
     CDS             complement(237..1403)
                     /locus_tag="PLK75_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOY40017.1"
                     /translation="MDLKNFTIAYAYKSKKELKSALFVYRIITRPFLVRFGKLMLDTA
                     LTLRIPIKPFTGFMFRHFCGGEKLDEVINISDCIEKFNVKVIPDFSKEGASEESAYEE
                     LISEVKKVIDISAENPNIPFAVFKPTGLIQAEYLKQHVPIDHSEIIAYRSRLDEIFGY
                     AAFKKTKVFIDAEDYWYQDRIDEMVLDFMRKYNKNTPVVFTTLQMYRHDRLDYLKTLT
                     ALARREGFKLGIKFVRGAYMEKERKRALEGSYPDPIHPNKEITDKAFDDAITYSVENI
                     DTIDVFCGTHNEKSILNLVNEIEKQQIAKNDPRIWFSQLYGMRDNQTFNLAAEGYNVA
                     KYLPYGPIREVMPYLVRRAEENSSMGSQAGEEIRMLKLALQFRNPKNKTFHQVP"
     gene            complement(1508..4666)
                     /locus_tag="PLK75_12040"
     CDS             complement(1508..4666)
                     /locus_tag="PLK75_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOY40018.1"
                     /translation="MNRYLILVLLLLSVAAGYSQTWREYHALYTDYYNRQIYDSAMVF
                     LNKAVSDCEDRDATSDTTYVVMISELGNLYGLMKMHNECIETITKANKLIIKNQGKNS
                     LYAENCLVLAGIYSSMGDYPASEKQLKESLDVLEKIFGNQSPQYLRCYIELPRLYVKM
                     RKLGDADGMYEECRKIAEKIYGKKSKQYLDLCSEEGVYLKKRRKYTESEILLKDCVDL
                     RKEIAGANDTAFASDYENLAMLYDKMGKYADAEVLMVQCLNIRKPALGEKHPAYLQAV
                     ENLGAVYKNSGQYARAEPYYIELKETAESMYGNADTVYADRCNALGLLYAAMGNYKNA
                     EPLYFEAKVIREANNGNTHPEYAKIINNIAALYIKLFDYEAAKPLLTESLEIKKKTEG
                     TETASYANSCNNLAYVYMNMGEFNSAEKLFKEALKINETRFGKAHINYLKAGNNLGNL
                     YIEMKSYNAAEKILLEIKEIASQKLGVTHPYYAEICNNLAKLYRIKGDVTQAKSAYLE
                     TISTANTIISNNFGLLTEQEKEMYFAGFDKFYDGFNAFALQTISNDSAIAEYVYNNTI
                     KTKGMLLKSSTALRFSILSSNDSVLLAKYDNWMLLKRQISDLYAQPVYERGNELTKLE
                     GEANTLEKQLIKDSKLFADSETEKKLTWTDIKSNLNPSEAAIEFIDFIDGDSVIYCAL
                     IVRQNSTYPEMIRLFEEKQLQQIFGKFLMNNVKYINSLYGQQNQANTKLYNLIWKPIE
                     AKLEGCTDVYISPSGLLHKISFPALCREQNVFLCDVYDINMSGSTAGIIRPSRHPITD
                     IHAFVLGGVEYNTEATDNNVWAYLPGTKEEADSITGIMQQGQTEFNYQSNRKASELVF
                     KQDATGYNVLHISTHGFFYPDPEELLHSRIQKQEKPDEETRASANGDWGFGMYAFAMN
                     RNPLMRSGLVLAGANNVWNKGAFPQPLVEDGVLTASEVAQLNLINVDLVVLSACETGL
                     GDIRGSEGVYGLQRAFRLAGVDYIIMSLWRVADKETKEFMTCFYQNLLECKDVEIAFT
                     KARSTMRQKYDPYYWAAFVLVE"
     gene            complement(4757..4978)
                     /locus_tag="PLK75_12045"
     CDS             complement(4757..4978)
                     /locus_tag="PLK75_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40019.1"
                     /translation="MKTKTQPVIEKLRSLFFGAMLIGLTLGFSACEDYTYDDDDDDDE
                     DVIQINVNENEKSFEMDSLNNIDLNKPEE"
BASE COUNT         1432 a         1092 c          934 g         1724 t
ORIGIN      
        1 aaatagaagc gataaggaaa agaatacaat tttcggaatg cataaaaggt agcaacattt
       61 gcagcgtttt aatgattctt aatcaacgaa attggtaatt tgtagttaag aaccaacatt
      121 tctcattggc cacagtgtca attattaaat ttctagtggg ccgataatat ggctgtaaca
      181 agcctgttta agctttacat atgcttatca ttcaaaacat tttcaagttt aattgactac
      241 gggacctggt gaaatgtttt gtttttcgga ttgcgaaatt gcaatgcaag tttcaacatt
      301 cgtatttcct cgcccgcctg tgaacccatc gaggagtttt cctcagcgcg tcttaccaga
      361 taaggcatta cttcccttat tgggccatag ggaagatatt tcgccacatt gtatccttcc
      421 gcagcaagat taaaagtctg attatcgcgc ataccataca gctgtgaaaa ccaaatacgc
      481 gggtcgttct ttgctatctg ctgtttttca atttcattaa ccaggtttaa aatggacttt
      541 tcgttgtggg taccgcaaaa tacatcaatt gtatcgatgt tttctaccga ataagtaatt
      601 gcatcatcaa aagccttgtc ggttatttcc ttgttcgggt gtatcgggtc gggatatgaa
      661 ccttccaaag cccgttttcg ttctttttcc atataggcac ctcttacaaa cttaataccc
      721 agtttaaaac cttcgcgcct tgccaatgca gttaaagttt tcaaatagtc taacctgtcg
      781 tgtcggtaca tttgaagagt ggtaaaaact accggggtgt ttttgttata ttttctcatg
      841 aaatcaagca ccatctcatc gatcctgtcc tgataccaat aatcttcggc atcgatgaac
      901 actttggttt ttttaaatgc tgcgtaacca aatatttcat ctaaccgact acgatatgca
      961 ataatttcag aatgatcaat cggcacatgc tgcttcaaat actccgcttg gattaatcct
     1021 gttggtttaa atactgcaaa tgggatattg gggttttctg cagaaatatc gatgactttt
     1081 ttcacttcag aaatcaattc ctcataagca gattcttccg acgccccttc tttcgaaaaa
     1141 tcagggatca ctttaacatt gaacttttcg atacaatccg aaatgttgat gacctcatca
     1201 agtttttcgc caccgcaaaa atgcctaaac ataaaacctg taaacggttt aatgggaata
     1261 cgcaaggtta gggcagtatc caacatcagt ttcccaaaac gaactaaaaa aggccgtgta
     1321 ataatccgat aaacaaaaag cgctgatttg agctcttttt tgcttttgta ggcataagct
     1381 atagtaaaat tttttaaatc catacggttg tggtgatttc tgtaaaacta caaatatttt
     1441 gattatggca tgtaatcccc taaaaaacct gattttaaaa aaccatcggt tacagaaaca
     1501 cgctgtctta ttctaccaat acaaaagctg cccagtaata cgggtcgtat ttctgcctca
     1561 ttgtgcttct ggcctttgta aaggcaattt ccacgtcctt gcattccaac aggttttggt
     1621 agaaacatgt cataaattct tttgtttctt tatcggcaac acgccagagg ctcataatga
     1681 tataatcgac tcctgcgagt cgaaatgccc gctgcaaacc atacactcct tcgcttcccc
     1741 tgatgtcgcc caatccggtt tcgcaagccg acaataccac aagatcgaca ttgatgagat
     1801 tgagttgagc tacttcgctg gcagttaaaa ccccatcttc cacgaggggt tgaggaaacg
     1861 cccctttgtt ccaaacattg ttcgctccgg ccaaaaccag gcctgaccgc attaatggat
     1921 tgcggttcat tgcaaatgca tacataccaa acccccagtc gccattcgct gatgcgcgcg
     1981 tttcttcatc gggtttttcc tgtttttgaa tacgcgagtg caacaattcc tcaggatcgg
     2041 gatagaaaaa tccgtgggtt gatatatgaa gtacgttata accggtcgca tcctgtttga
     2101 aaaccaattc actagcttta cggtttgact gatagttaaa ttccgtttgc ccctgctgca
     2161 taattcctgt aatactgtcg gcttcttctt ttgtacccgg aagataggcc catacattat
     2221 tgtcggtagc ttctgtattg tattcaaccc caccaagtac aaaagcatgg atatcggtga
     2281 tgggatggcg gctgggccgg ataatgccgg cagtacttcc cgacatatta atatcataaa
     2341 catcgcagag aaatacattt tgctcacggc aaagtgccgg gaaactgatt ttatgcaaca
     2401 atcccgacgg tgaaatatac acatcggtac agccttcgag ttttgcctca atgggtttcc
     2461 atatcaagtt gtaaagtttg gtatttgcct gattttgctg accgtaaagt gagttaatgt
     2521 atttaacatt attcatcagg aacttgccaa aaatctgctg aagttgtttt tcttcaaaca
     2581 agcgtatcat ttcgggatat gtgctgtttt ggcgcactat gagtgcacaa taaatcaccg
     2641 aatcgccatc aatgaaatcg ataaattcaa ttgctgcctc gctggggttt aagtttgatt
     2701 ttatgtcggt ccatgttagt tttttttcag tttcggaatc agcgaaaagt tttgagtctt
     2761 taataagttg tttttcaagg gtattggcct ccccctcgag ctttgtcaac tcattgcctc
     2821 tctcatacac gggctgcgca tacaggtcag aaatttgtct tttcagcagc atccagttat
     2881 cgtattttgc aagcaatact gaatcgttag aactcagtat gctgaatctg agagcagtgg
     2941 atgatttcag caacattccc tttgttttga tggtgttgtt gtacacatat tctgcaattg
     3001 cggaatcgtt gcttatggtt tggagtgcaa aagcattaaa accatcatag aacttatcga
     3061 aacctgcaaa atacatttct ttttcctgtt cggtaagcag tccgaaatta ttgctaataa
     3121 tggtgttggc tgtgctaatg gtttcaagat acgccgattt tgcctgcgta acatcacctt
     3181 ttatcctgta taatttagca agattgttac atatttcagc gtaataggga tgtgttacac
     3241 ccagtttttg tgatgctatt tctttaatct cgagaagaat tttttcagca gcattatagc
     3301 ttttcatttc aatgtataaa ttccccagat tgttgccggc tttcagataa ttaatgtgag
     3361 cttttccaaa tcgcgtttca tttattttaa gggcttcttt gaagagtttc tctgctgaat
     3421 tgaattctcc catattcata tatacgtaag caaggttatt gcagctattt gcatatgatg
     3481 ctgtttcggt tccctcagtt tttttcttta tttccaggct ttcggtcagt agtggtttag
     3541 cagcctcata atcaaatagt ttaatataga gtgctgcaat attgttgatg atttttgcgt
     3601 attctggatg agtgttccca ttattggctt cgcgaataac tttggcttcg aaataaagtg
     3661 gttcagcatt tttgtaattc cccattgcag catacagcag gcccaaagcg ttacatctgt
     3721 cggcatacac tgtatcagcg tttccgtaca tagattctgc ggtttctttt aattcgatat
     3781 aatagggttc tgctctagca tactgacctg aattcttata cacagcaccc aaattttcaa
     3841 cagcctgcaa atacgccgga tgtttctcgc caagtgctgg ttttcttatg ttcaggcatt
     3901 gcaccatcag cacttccgca tccgcgtatt tccccatttt atcgtagagc attgccagat
     3961 tttcgtaatc acttgcaaac gctgtgtcgt tcgcccctgc aatttcttta cgcaaatcaa
     4021 cacaatcttt caaaagaatc tccgattcgg tatatttcct ccttttctta agatacactc
     4081 cttcttcaga acataaatcc aggtattgtt tgcttttttt gccatagatt ttttccgcga
     4141 tttttcggca ctcttcgtac ataccatcag catcacccag ttttcgcatt ttcacataca
     4201 ggcgtggcag ttcaatgtaa catcttaaat attgagggct ttggttgcca aatatttttt
     4261 ccaaaacatc gagcgattct tttaattgct tctcagaagc aggataatca cccattgatg
     4321 aatatattcc tgcaagtacc agacagtttt cggcatataa actgttcttt ccctggtttt
     4381 tgataataag cttatttgcc ttggtaatgg tttcgatgca ctcattatgc attttcatta
     4441 acccgtaaag gtttcccaat tccgatatca tgactacata ggtagtatcg gaagttgcat
     4501 cccggtcttc gcaatccgaa acggctttat taagaaaaac catcgcagaa tcatatattt
     4561 gcctattgta ataatcagtg tataaagcat gatattcgcg ccaggtttgg gagtatcctg
     4621 ccgctacgct taacagcaat aaaaccaata ttaagtacct gttcatatgt tatgaataaa
     4681 gtaattgcag catttgattt actgcaatca ctttaaaaaa ttaagtcctt acaaatataa
     4741 cacaaaaaaa cgagttttat tcttccggct tgttcaaatc gatgttattc aggctatcca
     4801 tttcaaaact cttttcgttt tcgttaacat taatctggat tacatcctcg tcatcgtcat
     4861 cgtcatcgtc gtaagtgtaa tcttcgcagg ccgaaaagcc aagcgttaag ccaatcagca
     4921 ttgctccaaa aaacagtgag cgcagtttct caattaccgg ttgtgtcttt gttttcatag
     4981 tagcgtattg tttagttaat aataggtttc tttatagaag cttaacaaaa atgtttcctg
     5041 acatccttta tatttttcaa atataataca aatttatatc aaacatatac ataaagcaaa
     5101 tattttacat gtttttttat gagcaattcc tgacttcgac aatgcgccac cctaaaaatt
     5161 ctcccaaaag ttttcttcaa ac
//
LOCUS       DAOOQP010000162         5067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_19836_length_5067_cov_5.612729, whole genome shotgun sequence.
ACCESSION   DAOOQP010000162 DAOOQP010000000
VERSION     DAOOQP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19836_length_5067_cov_5.612729"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLK75_12050"
     CDS             complement(<1..741)
                     /locus_tag="PLK75_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HOY40020.1"
                     /translation="MKIICVRGFVLSIMLLTCISVSQAFVISDFYTVSDKHVGRGKNC
                     IVEMVPFAYISQSDPDTIPPDITCSPDIEANVDPGECTAVVNYAYPIATDASFTFDSG
                     IETFDYTGTEQTWVVPEGVTYVTIEAYGAQGGNGEYHGSIGVGGLGGYSIGKLAVTPG
                     QTLFIYVGQQGPSNTGNNRLTAFNGGGAGYGDSNGIRGSGGGASDVRVGGNTFYDRVI
                     VAGGGGGACCYTNGGDGGGLSGGGGTGNF"
     gene            977..1918
                     /locus_tag="PLK75_12055"
     CDS             977..1918
                     /locus_tag="PLK75_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY40021.1"
                     /translation="MKKILVTGGAGFIGSHLCEKLLNEGNEVICLDNFFTGSKSNIVH
                     LLGNKYFELVRHDVTMPYFIEVDEIYNLACPASPIHYQYNPIKTIKTSVMGAINILGL
                     AKRIKAKVLQASTSEVYGDPTVHPQPESYWGNVNPIGSRSCYDEGKRCAETLFFDYHK
                     QNNVRIKVIRIFNTYGPRMHPNDGRVVSNFIVQALQGKNITIYGDGTQTRSFQYVDDL
                     LVGMTLMMNTPDSVTGPVNIGNPHEVTMLELAEMVLRLTESKSKLVFMALPEDDPIQR
                     KPDITIAKNLLNNWDPKVKLEDGLIETIEYFRKSLSL"
     gene            1915..2802
                     /gene="rfbA"
                     /locus_tag="PLK75_12060"
     CDS             1915..2802
                     /gene="rfbA"
                     /locus_tag="PLK75_12060"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOY40022.1"
                     /translation="MKGIILAGGAGTRLHPITKSISKQIIPVYDKPMIYYPLSTLMLA
                     NIRDILIISTPTDIGLYETLFGSGSQLGLNISYAIQPSPDGLAQAFIIGEKFIGNDKV
                     CLVLGDNIFYGQGFGHLLKQASQLEDGALVFGYYVNDPQRYGVAEFDKNWKVISLEEK
                     PENPKSNFAVTGLYFYSNDVVEKAKNLKPSKRGELEITDLNNLYLAENRLAIQLLGRG
                     FAWLDTGTPQSLLQAANFIETLEQRQGLKASCIEEIAYHRGFINKEQLLKLAHDLGKS
                     DYGKYLLKIANSENIPGWK"
     gene            2793..3353
                     /gene="rfbC"
                     /locus_tag="PLK75_12065"
     CDS             2793..3353
                     /gene="rfbC"
                     /locus_tag="PLK75_12065"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOY40023.1"
                     /translation="MEIIPTPIPGLLIIEPKVFGDPRGYFMETWSKAVFDKYNIPCSF
                     VQDNESMSNYGVVRGLHYQLEPYAQAKLVRVVSGSVYDVALDIRKGSPTFGKWFGLLL
                     SGENKKQFFVPRGFAHGFAVLEDKTIFSYKCDNLYNKEHERGISFADPALGISWPIDF
                     AKAIISEKDKHNPSFDQAEYNFTFRN"
     gene            3396..3902
                     /locus_tag="PLK75_12070"
     CDS             3396..3902
                     /locus_tag="PLK75_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOY40024.1"
                     /translation="MENKNLLDILKSAILMEKRGQALYEEVATKTKDPDVKRIFEIMA
                     DEEKLHAKMLGEQFKNFSAHGGFLKQNLSTSTADHEIADLVFSGKISKNIGAAGFEAA
                     AINAAIDMETRSIEVYRQSSQSAADPNEKALFQWLADWEGGHYELLLKLDNDLKEKIW
                     SDNQFWPF"
     gene            4038..>5067
                     /locus_tag="PLK75_12075"
     CDS             4038..>5067
                     /locus_tag="PLK75_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOY40025.1"
                     /translation="MKKLALFSISFLFAVSAAFTQTELDDRASVCFNKGWGFATKDSS
                     IMTNLRFRIQNRFSMFSMSDSDLSINETEFKVRRLRLRFDGFVFDHKISYLFQLAFSR
                     DDIDWDKNKFPSLVRDAMVFYKPISNLQIGFGQGKLPGNRQRVISSGQQQFADRSIVN
                     SNFTLDRDFGFFVSISQPVGIGLFRLRAAVTSGEGRNIPKGDANFAYTGRIEYLPFGI
                     FTNDGDYFEGDLDREPKPRLSLAGGYSFNKGAIRTGGQLGSFMDSGHDIMTLFADMVF
                     KFRGWAFSSEFIKRDIHGSAFVTLTDGTPAYIVTGYGINTQLSYCFANHIEIAARHSV
                     IIPDNEIRS"
BASE COUNT         1554 a         1070 c         1047 g         1396 t
ORIGIN      
        1 gaagtttccg gttccaccgc caccacttaa gcctccacca tctccaccat tggtatagca
       61 gcatgctccg ccaccccctc cggcaacaat gacacgatca taaaaggtat tgccaccaac
      121 tctaacatca gatgcaccac caccactacc cctaatacca ttgctatcgc cataaccagc
      181 cccacctccg ttaaaggcgg ttagcctatt gttcccggta ttagacggac cctgctgacc
      241 aacgtaaata aataatgttt gaccaggtgt aactgctaat ttcccaatgg aatatccccc
      301 aagtcctcca acaccaatac ttccgtgata ttctccattt cctccctgag ccccgtatgc
      361 ttcaattgta acataggtaa ccccttcggg tacgacccag gtttgttcag taccggtata
      421 atcgaaggtt tcaattcccg aatcaaacgt aaaagatgca tcagtcgcta tcggatatgc
      481 atagttcaca accgctgtgc attctcctgg atcgacatta gcctcaatat caggagaaca
      541 tgttatatca ggaggaatag tatcgggatc agattgtgaa atatatgcaa atggaaccat
      601 ttcaacaata caatttttac ctctaccgac atgcttatcc gaaacagtat aaaaatcgct
      661 tataacaaaa gcttgactta ccgaaataca agttaaaagc atgattgata aaacaaatcc
      721 cctcacacaa attattttca tgattcaatg agtttttaca tgtaaattta cgataaatat
      781 cgattacaac cgacttgcca gaacaaatta gttatgagca gagttgattt gataacttat
      841 gtagaactta aaaagcaaac tccgatagga agtcattgat tgcttctttt gaacaataat
      901 aataagatta aatctaactg aattaaataa gcactgttgt attgatttaa tctatttttc
      961 ttaattttgc catattatga aaaagatatt agtaaccggc ggtgctggat ttatcggatc
     1021 tcatctttgc gagaagcttc tgaatgaagg caatgaggtg atatgcctcg ataatttctt
     1081 tacaggttct aaaagtaaca ttgtgcattt gctcgggaat aaatatttcg agttggtgcg
     1141 acacgatgtt acaatgccct attttattga agttgacgaa atttacaatt tggcttgccc
     1201 tgcctcgcca attcactacc agtacaaccc tataaagaca attaaaacct cggtgatggg
     1261 tgctatcaat attctgggac ttgccaagcg cattaaagcc aaagtattac aagccagcac
     1321 cagcgaggtg tatggagatc ccacagtgca tccgcaaccc gaatcgtact ggggcaatgt
     1381 gaaccctatc ggaagccgtt cgtgctacga tgaaggaaaa cgctgtgccg aaaccctgtt
     1441 tttcgattat cacaagcaaa acaatgtccg tataaaggtc atccgtattt tcaacactta
     1501 tggtccgcgt atgcatccta acgatggcag ggtagtttct aatttcattg ttcaggcttt
     1561 acagggtaaa aacatcacta tttatggtga cggaacccag acgcgcagct ttcaatatgt
     1621 cgacgacctg ttggttggca tgacgttaat gatgaataca cccgattcgg taacaggccc
     1681 ggtgaatatt ggaaatccac atgaggttac tatgctcgaa ctggctgaaa tggttctgcg
     1741 tctgactgag tccaaatcga agcttgtttt catggcattg cccgaggatg atcccattca
     1801 acgcaaaccc gatattacca ttgcaaaaaa cctcttgaac aactgggacc ctaaagtaaa
     1861 actcgaagat ggcctgattg aaacaattga atatttcaga aaaagtcttt cattatgaag
     1921 ggaattattt tagccggtgg tgcaggcacc cgtttgcacc caatcacaaa aagcatttcg
     1981 aaacagataa tcccggttta cgacaaacct atgatttatt atccgttgtc gacactgatg
     2041 ctggcaaata tcagggatat cctgataatt agtaccccta ccgacatcgg cttgtacgaa
     2101 acattgttcg gatcgggaag tcaattggga ctgaatattt cttatgctat acaaccctca
     2161 cccgatggac tggcacaggc atttattatt ggtgaaaagt tcataggcaa cgacaaagtt
     2221 tgtctggtgc tgggcgataa cattttctat ggacagggat ttggtcatct gttgaaacag
     2281 gcttcccaac tagaagacgg agcacttgtt ttcgggtatt atgtgaacga tccgcagcga
     2341 tatggtgttg cagagttcga taaaaactgg aaagtgatca gccttgagga aaaacccgaa
     2401 aacccaaaat caaattttgc agttacagga ctatacttct atagtaacga tgttgttgaa
     2461 aaagcaaaaa acctgaagcc ttcgaaaaga ggtgaactcg aaatcaccga cctgaataat
     2521 ctttaccttg ccgaaaaccg gttggcaatt caactgctcg gacgtggatt tgcctggctt
     2581 gatacgggaa caccgcaaag ccttcttcag gctgcaaatt ttattgaaac actggagcag
     2641 cgacaaggtt taaaagcctc atgtatcgaa gaaattgctt atcacagggg gtttatcaac
     2701 aaagaacagc tgctgaaact tgcacatgat ctggggaaaa gtgattatgg aaaatatctg
     2761 ttaaaaattg caaactctga aaatataccc ggatggaaat aataccaaca cctatcccgg
     2821 gcttactgat aatagagccc aaagtctttg gcgaccctag gggatatttc atggaaacgt
     2881 ggagtaaggc tgtattcgat aaatacaata ttccttgcag ttttgtgcaa gataatgaat
     2941 cgatgtcgaa ttacggagta gtgagaggct tgcattacca attggagcct tatgctcagg
     3001 caaagctggt gcgggttgtt tcgggaagtg tttacgatgt ggcactcgac atccgcaaag
     3061 gttctcccac attcgggaaa tggttcggac tgttgctatc gggcgagaat aaaaagcagt
     3121 tttttgtacc cagaggcttc gcccatggat ttgccgttct cgaagacaag acaatattta
     3181 gctacaaatg cgataacctg tataataaag aacatgagag aggtatcagc tttgctgatc
     3241 cagctttagg aatttcatgg cctatcgatt ttgcaaaggc aatcatatcg gagaaagata
     3301 aacacaaccc cagcttcgac caagccgagt ataattttac attccgaaac taaacatttt
     3361 atcgtagtat ttgtttataa tcaaaacaac acaacatgga aaataaaaat cttctcgata
     3421 ttttaaaatc cgccatttta atggaaaaac gcggtcaggc actttatgaa gaagttgcga
     3481 caaaaaccaa agaccccgat gtaaaacgaa ttttcgagat tatggcagat gaagaaaaac
     3541 tgcatgcaaa aatgcttggt gaacaattta aaaacttcag cgctcatgga ggctttttaa
     3601 agcaaaacct atcgaccagt actgccgacc atgaaattgc agatttggtt ttttcgggta
     3661 aaatttcgaa gaacattgga gctgccggat ttgaagctgc cgctattaac gctgcaatcg
     3721 atatggaaac ccgctcaatt gaagtgtacc gccaaagctc acaatcagct gctgacccaa
     3781 acgaaaaagc gctgtttcaa tggctggccg attgggaagg aggccattat gaacttttgc
     3841 tcaaacttga caacgatttg aaagaaaaaa tatggtctga caatcaattc tggccattct
     3901 aattttttac catttcattc tgttgaggca tttatcagca aattagtata aattgctatg
     3961 taaacttaac ctaaacttaa ctatatcgtc aggttaagct attatcagct aaatactttt
     4021 gacgcaaaac ttattgcatg aaaaagttag ctctgttttc gatttccttt cttttcgcag
     4081 tttcggcagc atttacccaa actgagctcg acgaccgcgc aagtgtgtgt ttcaacaaag
     4141 gctggggctt tgcaacaaaa gacagcagta taatgacgaa cctgcggttt cggattcaaa
     4201 accgtttttc gatgttcagt atgtcggaca gtgatttatc aataaacgaa acggaattca
     4261 aagtaaggag acttcgcttg cgtttcgatg gctttgtttt tgatcataaa atcagttatt
     4321 tgtttcagtt ggctttttcg agagatgata tcgattggga taaaaacaaa tttcccagtt
     4381 tagtacgcga tgccatggta ttctacaagc ctatatcgaa tttgcagatt ggatttggtc
     4441 agggaaaact gccgggtaac cgtcaacggg ttatcagcag tgggcaacag cagtttgccg
     4501 accgaagcat agtaaacagt aattttaccc ttgaccgcga tttcgggttc ttcgttagca
     4561 tttcgcaacc ggttggaatt ggattattcc gtctacgagc tgcagtaacc agcggagaag
     4621 gcagaaacat tccgaaaggg gatgctaatt ttgcctacac cgggcgtatc gagtatttac
     4681 cttttggcat attcacaaac gatggcgact atttcgaagg tgatttagac cgggaaccaa
     4741 aaccccggct ttctcttgcc ggtggctaca gcttcaacaa aggagcaata agaacagggg
     4801 ggcaactggg cagctttatg gatagcggac acgatatcat gacacttttt gcagatatgg
     4861 tgtttaaatt ccgtggatgg gcattcagca gcgagttcat aaaacgtgat atacatggta
     4921 gtgcattcgt tacccttact gacggcactc ctgcatatat tgttaccggt tatggaatca
     4981 acacccagct gagttattgc tttgccaacc atatcgagat agccgcccgt cattccgtta
     5041 ttatacctga taacgaaatt cgttcag
//
LOCUS       DAOOQP010000163         5031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20018_length_5031_cov_5.686495, whole genome shotgun sequence.
ACCESSION   DAOOQP010000163 DAOOQP010000000
VERSION     DAOOQP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20018_length_5031_cov_5.686495"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLK75_12080"
     CDS             <1..620
                     /locus_tag="PLK75_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40026.1"
                     /translation="LPNLPYPEYRVPYNSLAALDSTHIFVAGGENYYKGLTSELEPGA
                     NQWIHHSFDNEIRDILFVDENLGFFAAYGQVLITEDGGNTFSYLEIDNEYFYDLEYFQ
                     NTIWLLGRYGKLYTSGDSGNSWTLQKEFGEMTLQDLWICNTDSYICGGGGFLLISRDQ
                     GESWYSVSGCGNTNLNSISVTPSGEVWVGGDDGKLFVLNRHRGAE"
     gene            797..1648
                     /locus_tag="PLK75_12085"
     CDS             797..1648
                     /locus_tag="PLK75_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40027.1"
                     /translation="MSINHLRAALLPLLLASVLSVYAGDSIPCGYNFLRMGIGFTKNS
                     VLDQVNTCLPYTGPGIGISLGYERCRKNCIEVGNDFAFGTLSPYNLPEYDNQVSFYYD
                     NFYAKYLWRLPIHKFFGLNLFAGPEIRIQFGMRINGAALANSVLAYDFATSTGVALML
                     TRDFSLHCLFKKAGDRKCISVSAKLSHPLLAMVHTPPYIGMPENLLQQNASLFDLSNH
                     YESVINRFINPDFCLSLSYNMKNGNALALHYRYSYFATYPEYNPTKAAYNYFGFSLLF
                     NLKKPCS"
     gene            1645..2661
                     /locus_tag="PLK75_12090"
     CDS             1645..2661
                     /locus_tag="PLK75_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY40028.1"
                     /translation="MKKIANLFLIFAGLASLGACERAVLDQPPSSKKDVFNFLWKSID
                     ERYSFFEMKHIDWDSVYTVYEPKITETMSEEAFFDTLSLLIDELRDGHSGINSFNNGH
                     QNVCFFFNSPENFNERIITDFYFKGYGNRTGAFQHAHIRHRNIGYIYYGSFSEDVTDE
                     DMEYILDYYSDAMGLIIDVRNNFGGTVENVYDIACHFTNEPVTVLGSQLKIGPGHEDF
                     TNITYTTIEPRGTFWTKPVIVLTNRKTYSAGSIFSLVMSQMPSVMVVGDTTGGGLGLP
                     IGTELPNGWQMHCAGSRILSPEGVCYELGVPPDVQQNMDPEQGYDDIIEKAADIIMFG
                     NYRD"
     gene            complement(2667..3218)
                     /locus_tag="PLK75_12095"
     CDS             complement(2667..3218)
                     /locus_tag="PLK75_12095"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOY40029.1"
                     /translation="MSLIHEQQIKEDTVIQVCRLMAVAARTAPKARGNDNLEIAILQG
                     ETIVQLSNKMEEISTAMQMPFFSRDAANLLLSQAVLLIGVKINTTGLNFCGYCGMETC
                     ENKQKFGNVPCAFNLIDLGIAAGSAATDTAEPVAAAHHIDNRVMFSAGKAALELGLMS
                     ENIRDIVAIPLSVQGKSPFFDRK"
     gene            complement(3223..4170)
                     /locus_tag="PLK75_12100"
     CDS             complement(3223..4170)
                     /locus_tag="PLK75_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY40030.1"
                     /translation="MKHVLLLDTAHSILTEKLTEKGFTVDEKPSMQRHELIDAAHLYN
                     GIILRSRITIDKEIIDKAVNLEFIGRIGSGMESIDTKYAAAKNIACFNSPEGNRDAVG
                     EHSIGLLLNLLNNICRANMEVKEGLWLRESNRGIEIKDRTVAIIGYGNMGSAFAARLS
                     GFGCKVIAYDKYKFGFSSEHVNEVSMDYIFAEADILSLHIPLTSETHYLVDSAFIAKF
                     AKPIVLINTSRGPVVKTAHLAEALINGKISSAALDVIEYEESSFEKTDDMLNHDAFRN
                     LISSGKVIITPHIAGWTIESKYKLAAVLAEKIIRFYQKE"
     gene            complement(4212..4571)
                     /locus_tag="PLK75_12105"
     CDS             complement(4212..4571)
                     /locus_tag="PLK75_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40031.1"
                     /translation="MKNTVPAFLVATLIFIACETIYNYDLNYTITAGNQATRNAIDVC
                     IPGSVNLRKGTIDNEMILIFRNLDASEQQISDSLRKDIQPANDTSIILNIDISALDPE
                     NGLTTYTYNHTYSLTGL"
     gene            complement(4568..>5031)
                     /locus_tag="PLK75_12110"
     CDS             complement(4568..>5031)
                     /locus_tag="PLK75_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40032.1"
                     /translation="TLEPSTNQDVELFKEETGISFPCFSNIDNEPVYEYFEVTYYPQI
                     HIICPDKRRAEISFFEMIESLGFCEPNYINQNQINSPFIVTHEYGKINITNSSSTSAF
                     CVITDVTGKTLYSGDLNPGSLCLPVNHTDSNFYILRFYSNGETYCKKIFIQ"
BASE COUNT         1420 a          977 c         1059 g         1575 t
ORIGIN      
        1 acttaccaaa cctgccatac cccgagtaca gggttccata taacagcctg gctgctcttg
       61 attcgaccca tatatttgtt gcaggtggcg aaaactatta taaaggatta acatcagaat
      121 tggagcccgg tgccaatcag tggatacatc atagtttcga taacgaaatc cgggatatat
      181 tatttgttga tgaaaacctt ggcttttttg ctgcctacgg acaggtgcta ataaccgaag
      241 atggcggaaa cacctttagt taccttgaaa tcgacaatga atatttttac gacctcgagt
      301 atttccaaaa tacaatatgg cttctcggac gttatggtaa gctctacaca tccggtgata
      361 gcgggaatag ttggactctc caaaaagaat tcggtgaaat gactttacaa gacttatgga
      421 tttgcaacac tgactcttac atttgcggtg gcggaggttt tcttcttata agccgcgatc
      481 agggtgagtc gtggtacagt gtttcgggat gtggcaatac aaacctgaac agcattagtg
      541 tgacaccctc gggagaagtc tgggtcggtg gcgacgatgg aaaactattt gtactaaacc
      601 gacatagagg tgctgagtaa accgttttcc ttcggggaac acttccataa ttaaataaaa
      661 atgctttttc cataattgga aagtaacatt tgaaattttc aaacaaaaga acttcttatt
      721 atttccagtt ctaagatgta gtttcaactt aaaacaggta ttttagcgta aaatattcat
      781 tctaaagcat caatatatga gtataaatca tttgagagcg gcacttctgc cattgttact
      841 ggcaagtgtg ctttctgttt atgcaggcga cagtattccc tgcggttaca actttttgcg
      901 catgggcata ggatttacaa agaactccgt actcgatcag gtgaacacct gtttgccata
      961 taccggcccg ggtattggaa tttcgctggg atatgaaagg tgcaggaaaa actgcatcga
     1021 agtgggtaat gattttgcat tcggaaccct atccccttac aacttgcccg aatatgacaa
     1081 ccaagtgagt ttttattatg acaattttta tgcgaaatac ttatggcgtt tacccataca
     1141 taaattcttt ggtttgaact tgtttgccgg ccccgaaata aggattcagt ttggaatgcg
     1201 gattaatgga gctgctcttg caaactcagt tttggcatac gactttgcta catcaacggg
     1261 cgttgcgctt atgttaacac gtgatttttc gctgcattgc ctttttaaaa aagcaggtga
     1321 tcgcaaatgt atttcggtta gcgcaaagtt gtcgcaccct ttacttgcca tggttcatac
     1381 gccaccttat attggaatgc ctgaaaacct attgcaacaa aatgcctctt tgttcgattt
     1441 gtcaaatcat tacgaaagtg taataaaccg atttattaat cctgatttct gcttaagcct
     1501 gagctacaat atgaaaaatg gtaatgcatt ggcattgcat taccggtata gctattttgc
     1561 aacttatccg gaatacaacc cgaccaaagc agcctataat tattttggct tttctctgtt
     1621 attcaatctt aaaaaaccat gctcatgaaa aaaatagcga atttgttttt gatttttgcc
     1681 ggacttgctt cattgggagc ttgcgaaagg gcggtattgg accagccgcc ttcatcaaaa
     1741 aaagatgttt ttaatttctt atggaaatcg attgatgaaa ggtacagctt tttcgaaatg
     1801 aagcatatcg attgggattc ggtgtacact gtgtatgaac cgaaaattac tgaaaccatg
     1861 agcgaggaag cctttttcga caccttgtcg ttactgattg atgaactgcg tgatgggcat
     1921 tcaggtatta attctttcaa caacggtcat caaaatgtgt gcttcttctt taacagtcct
     1981 gaaaacttta acgaaagaat tattacagat ttttatttta aaggttacgg gaaccgtacc
     2041 ggagcttttc agcatgcaca cataagacac cgaaatatcg ggtatattta ttacgggagt
     2101 tttagtgaag atgtaaccga tgaggatatg gagtatattc tggattatta ttccgatgcg
     2161 atgggattaa ttattgacgt caggaataat tttggaggta cagttgaaaa tgtgtacgat
     2221 attgcctgtc atttcacaaa cgaaccggta actgttttgg gatcgcagct aaaaatcggc
     2281 ccagggcatg aggattttac caatattacc tataccacaa ttgaacccag agggactttc
     2341 tggacaaaac cggtaattgt tttaaccaac cgaaaaacct atagtgccgg ttcaattttt
     2401 tcgctggtta tgagccaaat gccttcggta atggtggttg gcgacactac gggtggtggt
     2461 ttgggtttac ctattggaac ggaattgccc aacggctggc agatgcattg tgccggaagc
     2521 cgtattttat cacccgaagg tgtttgctat gaattgggtg ttcctcccga tgtacagcaa
     2581 aatatggacc ccgagcaagg ctatgacgat attattgaaa aagccgccga tattattatg
     2641 ttcggaaatt atagggatta gcttgcttat ttcctgtcga agaatggact ttttccctgt
     2701 accgacagtg gaatggccac aatatcgcgg atattttcgc tcatcagacc caattccaat
     2761 gcggccttcc cggctgaaaa cattaccctg ttatcgatgt gatgtgctgc agcaaccggt
     2821 tctgcagtat cggttgctgc agaaccggct gcaatgccca ggtcaattag gttgaatgcg
     2881 cagggtacat tcccaaattt ctgcttgttt tcgcaggttt ccatcccgca ataaccgcaa
     2941 aagttcagtc ctgttgtgtt aattttaact ccgataagca acacagcctg cgatagtaac
     3001 agattggcag cgtcgcggct aaaaaatggc atctgcatag cagtcgaaat ttcctccatc
     3061 ttgttggaaa gttgcactat ggtctcgcct tgaagaatag ctatttcgag gttatcattt
     3121 ccacgcgctt tgggtgctgt gcgggcagct actgccataa gcctgcaaac ctgaattacg
     3181 gtatcttctt taatttgttg ctcgtgtatt aaactcatag ggttattctt tttggtaaaa
     3241 cctgattatt ttttcggcaa gtactgctgc cagcttatat tttgattcaa ttgtccagcc
     3301 tgcaatatgt ggggttatta tcacttttcc ggacgatatt aaattacgaa aagcatcgtg
     3361 attcaacatg tcatcggttt tttcaaacga tgattcttca tattcaataa cgtcgagggc
     3421 agcgctcgaa attttcccat tgattagggc ttcggcaaga tgcgcagttt ttacaactgg
     3481 tccccttgat gtgttgatta gtacaattgg cttggcaaat ttggcgataa aagcagaatc
     3541 gacaagataa tgcgtttcag aagtaagtgg tatatgcaaa ctgaggatat cggcttcggc
     3601 aaaaatgtaa tccatactaa cttcgtttac atgttcactg gaaaatccga atttatattt
     3661 gtcgtaggca attaccttac agccaaaccc tgacaatcgc gcagcaaagg cagaccccat
     3721 attcccgtaa ccgatgattg ctacggttct atctttaatt tcaatgcccc gattggattc
     3781 acgcagccaa agtccttctt ttacttccat gtttgcacgg cagatattat taagaagatt
     3841 cagcagcaac ccaattgagt gctccccaac agcatcgcga ttcccttcgg gagagttgaa
     3901 acatgcaata tttttggctg ctgcatattt tgtgtcgatg ctttccatgc ctgaaccgat
     3961 tcgtcctatg aactctaggt ttacagcctt gtcgataatc tctttgtcga tggtgatgcg
     4021 gctgcgtaaa ataattccat tgtaaaggtg cgctgcatcg ataagctcat gtcgctgcat
     4081 agaaggtttc tcatcaacag taaacccttt ttctgtgagt ttttctgtaa gaatgctgtg
     4141 ggctgtatca agcagtaaaa cgtgtttcat tgaatcctga tttttcgggc taagtgagtt
     4201 ataaatactt tttataaacc tgttaatgaa taagtgtgat tataggtata tgtagttagc
     4261 ccattttccg ggtccaaagc actgatatca atattcaata ttatcgaggt atcgtttgca
     4321 ggttgaatgt cttttcgtaa ggaatcggaa atttgttgtt cacttgcatc taaatttctg
     4381 aatattaata tcatttcgtt gtcgatagtg ccttttctca aattaacaga cccgggaatg
     4441 caaacatcaa tggcattacg ggttgcttgg ttcccagcag taatagtgta attgaggtcg
     4501 taattataga tggtttcaca agctataaaa atcagagttg ctaccaaaaa tgccggaaca
     4561 gtatttttca ttgtataaaa atctttttac aataggtttc tccgttagaa taaaatctga
     4621 gtatataaaa attactatcg gtatggttaa caggcaagca taggctacca gggttaaggt
     4681 ctcccgaata aagagttttt cccgtaacat cagttattac acaaaaggcc gaagtagagc
     4741 tactattggt aatattaatt tttccgtact catgggtcac aatgaatggt gaattaatct
     4801 ggttctgatt aatgtaattt ggttcacaaa aaccaaggct ttctatcatt tcgaagaaac
     4861 ttatttcggc tcttctttta tcgggacaaa ttatatgaat ctgagggtaa taagttactt
     4921 cgaaatactc atacaccggt tcattatcaa tgttcgaaaa gcaggggaac gaaatacccg
     4981 tttcttcctt gaacaattcg acatcttggt tggtagaagg ttcaagtgtg c
//
LOCUS       DAOOQP010000164         4906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20631_length_4906_cov_4.985982, whole genome shotgun sequence.
ACCESSION   DAOOQP010000164 DAOOQP010000000
VERSION     DAOOQP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20631_length_4906_cov_4.985982"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLK75_12115"
     CDS             <1..213
                     /locus_tag="PLK75_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOY40033.1"
                     /translation="ILYMGDDLPDYRVMQRVGVATCPLDAAQEIKAISLYVSQYKGGE
                     GCVRDIIEQVLRTQGKWMTNEAFVLC"
     gene            249..1184
                     /locus_tag="PLK75_12120"
     CDS             249..1184
                     /locus_tag="PLK75_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOY40034.1"
                     /translation="MVALTMYAMLYGIAHPVLEYYGGEIVTSPLQFIFLVLSFVFLAA
                     AGYVINDYFDRKADLVNRPDTIVVGIHIKRRWAMVFHTVFNIIGVILGFAVAFSIGKW
                     WFGFLFIAFSLILWWYSSNLKKLPVAGNLTVAILGAVVPLTIAGFQYYGTIRVLGILP
                     VNISIAMKEIFDISVGFAVFAFMFNFIRELVKDMEDIEGDKAMYCKTIPIVLGIRLTK
                     YLASLLCFAGVFLVLLSYVAYIRNLSYMTNDFISIWYVPVLVALPLFYCGIRLIFSEK
                     KSDYKMISNILKLNMLTGILYTLVMFLIIISNTVK"
     gene            1181..1756
                     /locus_tag="PLK75_12125"
     CDS             1181..1756
                     /locus_tag="PLK75_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOY40035.1"
                     /translation="MTGKKIYNIILGSASPRRRALLEEMGIPFRIVRIDFPEENFPSH
                     LYREEIALYLAAEKSKHFGNLAENDLLITADTIVWHNDTVLGKPLHYSDAFQMLKRLS
                     NQCHDVITGVCLRSSEKTHCFFAETKVKFADVSNDDIKTYIEKYKPFDKAGAYGIQEW
                     IGLIGIEWVQGSYFNVVGLPTHRLFVELQNF"
     gene            1765..3924
                     /locus_tag="PLK75_12130"
     CDS             1765..3924
                     /locus_tag="PLK75_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY40036.1"
                     /translation="MKKLLFILIALISWLFSIAGEGMWIPLLLEKYNYEDMQAKGFRL
                     TPEDIYSINHSSLKDAVVIFGGGCTGEVISDQGLIITNHHCGFGTIQRHSSLEHDYLT
                     DGFWAQTKEEELSSPWLKVTFLVRMDDVTAQVLNGVSNKMTEAERDAVINKNIGEITS
                     RAVTGTHYEAVVESFYYGNQYFLFINEVFKDVRLVGAPPSSIGKFGGDTDNWMWPRHT
                     GDFSLFRIYSDPNGKPAAYSKNNIPLKPRKHMSISLKGVQEGDFTMVLGYPGSTEQYI
                     PSYAVDITMNQTDPNRISLREARLRVINQAMDNDRGIRIQYANKAASIANGWKKWIGE
                     VRGLKRLNAVHQKELFERAFQKWTASNRTLTQKYGDILSKYESLYTAAKPYYHAYDYL
                     AEAGFGIEVISFAGKFNSLYLAAKSGSSKEDLNGIIEKLKPGIAAFYKDYQAFIDHEV
                     MKSLLKMYYKNVPSAYLPTFLCKNFLQNSDEMIEMMADNAFEKSIFTNQARLDKFLLD
                     FKPKNYKKLELDPVFIMYRNLIDLYINKIEPVIADTENELDSLNRVYMKAQMEFMADS
                     LFYPDANFTMRLTYGKVSEYKPCDAVTYKWYTTLDGIIEKDNPEIYDYDVPDKIKELY
                     KAKDYGRYADADGKLHVAFIATNHTTGGNSGSPVLNADGELIGVNFDRNWEGTMSDIM
                     YDPSMCRNISLDIRYALFVIEKYAGADHLIEEMTIVE"
     gene            3939..4385
                     /locus_tag="PLK75_12135"
     CDS             3939..4385
                     /locus_tag="PLK75_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOY40037.1"
                     /translation="MNVNKVILIGRLGKDPIVSHPQPELTKAEFTLATTESYKKDNQW
                     VDITEWHNITAWRSSGLYAEKHLKKGMLVYVEGKIRSNKWQDSNGNQRTTYEIVADNL
                     QKLEKTPTDAQHDTKNTFTASKNTLEEPSPDAGLMNGPNESDIVPF"
     gene            4386..>4906
                     /locus_tag="PLK75_12140"
     CDS             4386..>4906
                     /locus_tag="PLK75_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HOY40038.1"
                     /translation="MYQNSTEILFVLLNFAKLNLSFYITGTLVVVVLIIISGMVSSSE
                     VAFFSLSPKDKLNLSKNKSKSGKTAYKLLLKPEKLLGTILVTNNFVNVAIVILVTYLT
                     GPLFSFIDSETIKVIVQLVIVTAILLLLGELLPKVYASKFPLKTALKLALPLNVASKI
                     FSPVVYILVRSTS"
BASE COUNT         1495 a          952 c         1007 g         1452 t
ORIGIN      
        1 attttatata tgggcgacga ccttcccgac tatcgggtaa tgcagcgtgt gggcgtggcc
       61 acctgtccgc tcgatgcagc ccaggaaatt aaagccattt cgctctatgt gtcgcaatat
      121 aagggtggcg aaggctgtgt gcgcgatatc attgaacagg tgttgcgcac tcagggtaaa
      181 tggatgacaa atgaggcatt tgtattatgc tgagtttctt acgattaata cggttcccaa
      241 atttggtaat ggttgcgcta accatgtacg ctatgctgta tggtattgcc catccggttc
      301 tggagtatta cggaggtgaa attgtcacca gcccgttgca atttatcttt cttgtgctgt
      361 cgtttgtttt ccttgcagca gctgggtatg taataaacga ttatttcgac cgtaaagccg
      421 atttggtaaa cagacccgat actattgtgg taggaataca cataaaacgc cgctgggcaa
      481 tggtttttca tacagtattc aatattatcg gagttatact cgggtttgct gttgcttttt
      541 cgattggcaa atggtggttc gggtttcttt ttatcgcttt ttcattgata ctatggtggt
      601 attcttcgaa ccttaagaaa ctaccagttg cagggaatct taccgttgcc atattaggcg
      661 ccgtggtgcc tcttaccatt gccggatttc aatattacgg aacaatccgt gttctaggta
      721 ttttacctgt caatatttcc attgcaatga aagagatttt cgacatttca gtaggatttg
      781 ctgtgtttgc attcatgttt aatttcatta gggagctcgt gaaggatatg gaggatattg
      841 aaggagataa agccatgtat tgcaaaacaa tccctattgt gcttggaata agattaacga
      901 aataccttgc ttcattactt tgttttgccg gagtctttct ggttttactg tcgtatgttg
      961 cttacataag gaacctctcc tatatgacca acgattttat cagtatctgg tacgttcctg
     1021 ttcttgttgc cctgcctctt ttttactgtg gtatcaggct tatcttttca gaaaaaaaat
     1081 ctgattacaa aatgataagt aatatcctga aattaaatat gctcacaggt atcctctaca
     1141 ctttggttat gtttctgata attatatcaa acactgtaaa atgaccggta aaaaaatata
     1201 caatattatt cttgggtcgg caagtccgcg tcgcagagcc cttctcgaag aaatgggcat
     1261 tccttttcgg atagtcagaa tagattttcc tgaagagaac ttcccctccc acttataccg
     1321 tgaagaaatt gcgctttatc tggctgcaga aaagtcaaaa cattttggca atcttgctga
     1381 aaacgacttg ctgatcaccg ccgataccat tgtgtggcat aacgatactg tactggggaa
     1441 gcccttacat tactccgatg cttttcagat gctgaagcgc ctcagcaatc agtgccacga
     1501 tgttattacc ggtgtttgtc tgcgtagctc agaaaaaaca cattgcttct ttgcagagac
     1561 caaagttaaa tttgccgatg tcagcaacga cgatattaaa acatatattg aaaaatacaa
     1621 acccttcgat aaagccggag cctatggtat tcaggagtgg attgggctga ttggcattga
     1681 atgggttcaa ggttcgtatt ttaatgttgt aggattaccg acacaccgtt tatttgttga
     1741 acttcaaaat ttttaaaagt tttcatgaaa aaattactat tcattttgat tgcattaatt
     1801 tcctggcttt tctcaattgc cggagaaggc atgtggattc ccctactgct tgaaaaatat
     1861 aactacgaag acatgcaggc taaagggttt aggttaacgc ctgaagatat ttacagtatc
     1921 aatcattcat cactaaagga tgctgttgtt atatttggag ggggttgcac cggtgaggta
     1981 atttcagatc aggggcttat tataacgaac catcattgtg ggtttggcac tattcagcga
     2041 cacagcagcc ttgagcacga ctatcttacc gatggttttt gggcacagac aaaagaagaa
     2101 gaattgtcaa gtccatggct caaagttact tttctcgtaa gaatggacga tgtaacagcc
     2161 caggtgctca atggcgtttc caataagatg acagaagcag aacgcgatgc tgttattaat
     2221 aagaatatcg gtgaaattac ttcaagagcg gtaaccggaa cccattatga ggcagttgtt
     2281 gaatcgtttt attatggtaa tcagtatttt ttatttatca atgaggtctt taaagacgta
     2341 cgccttgtgg gtgctcctcc gtcctccata ggtaaatttg gcggggatac cgataactgg
     2401 atgtggccac ggcatacagg cgatttttca ttattcagaa tatattccga ccctaacgga
     2461 aaacctgcag catattcaaa aaataacatt ccactgaaac ccagaaagca tatgagcata
     2521 tcgctgaaag gagttcagga aggtgatttt acaatggtac tcggatatcc aggaagtacc
     2581 gaacaataca ttccttcata cgcagttgat attactatga atcaaaccga tccaaacaga
     2641 ataagcctgc gcgaagcccg tctcagggta ataaaccaag ccatggacaa cgataggggt
     2701 atccgcatac aatatgccaa taaagccgcc tctattgcaa acgggtggaa aaaatggatt
     2761 ggtgaagtca ggggattaaa acgtctgaat gcagtacacc agaaagagct ctttgaacgt
     2821 gcatttcaga aatggaccgc ttcgaaccga actttgacac aaaaatacgg tgatatcctc
     2881 tcaaaatatg aaagcttata tactgccgca aaaccatatt atcatgctta cgattatctt
     2941 gcagaagccg gatttggcat tgaggttatc agttttgccg gtaaattcaa cagtttgtac
     3001 ttggctgcca aaagcggttc cagtaaagaa gaccttaacg ggataattga aaagctcaaa
     3061 ccgggtattg ctgcatttta caaagactat caagctttca ttgatcatga agtaatgaaa
     3121 tcgttgttaa aaatgtacta taaaaatgtt ccgtccgcat atttgccgac ctttttatgt
     3181 aaaaattttc tgcagaatag tgatgagatg attgaaatga tggctgataa tgcatttgaa
     3241 aaaagcattt ttacaaatca ggcacggctc gacaaattct tattggattt taaacccaaa
     3301 aactataaaa agctggagct cgatcctgtg ttcatcatgt atcgtaacct cattgattta
     3361 tacatcaata aaattgaacc tgtgattgcc gataccgaaa atgaactcga cagtttaaac
     3421 cgtgtgtaca tgaaagcgca gatggaattt atggccgaca gccttttcta tcccgatgcg
     3481 aactttacga tgcgccttac ttacgggaaa gtttcggaat acaaaccctg cgatgctgtt
     3541 acctataaat ggtataccac gctcgacggt atcatcgaaa aagataaccc cgaaatttat
     3601 gattatgatg tacccgataa aataaaagaa ttatataagg caaaagatta cggcagatat
     3661 gccgatgccg atggcaaact acatgtggca ttcattgcta ctaaccatac cacaggcggt
     3721 aattccggaa gtcctgtgct caatgccgat ggtgaactga ttggggtaaa ttttgaccgg
     3781 aactgggaag gaacgatgag cgacatcatg tacgatccaa gtatgtgccg caatatttca
     3841 ctcgacatta ggtatgctct ttttgtaatc gaaaaatatg ccggagcaga ccacctaatt
     3901 gaagaaatga ccattgttga ataataatac ctaattatat gaatgtaaat aaagtaattt
     3961 taatcggacg cctcggaaaa gatcccattg tatcgcatcc ccaaccagag ttaaccaaag
     4021 ctgaattcac tcttgcaaca accgaatcgt ataaaaaaga caatcaatgg gttgatatta
     4081 ccgagtggca taatattacc gcatggcgct cctcagggct atatgccgaa aaacatctaa
     4141 aaaaaggcat gctcgtatat gttgaaggta aaatccggtc aaacaaatgg caggacagta
     4201 atgggaatca acgaaccact tatgaaatcg ttgcggataa tctgcaaaaa ctcgaaaaaa
     4261 ccccaactga tgcccaacac gacacgaaaa acacttttac cgcatctaaa aatacactgg
     4321 aagaaccatc acccgacgca ggcctgatga atggaccaaa tgaatcagac attgtacctt
     4381 tttagatgta tcagaattca actgaaatat tatttgtact gctgaatttc gcaaaactta
     4441 acctgagttt ttatattaca ggaactcttg ttgtagtggt attaattatt atttctggaa
     4501 tggtttcttc atcagaagtt gcattttttt cgttatcacc caaggacaaa ctaaatttaa
     4561 gtaagaacaa atcaaaatcg ggaaaaacag cttataaatt actgcttaaa cctgaaaagc
     4621 tgttaggtac aattttggta accaataatt tcgttaacgt ggctattgtt attcttgtaa
     4681 catatcttac cgggccactg ttttccttta ttgacagcga gactataaaa gtgattgtgc
     4741 agcttgttat cgttactgca attctcctac tgttgggaga acttctcccg aaagtgtatg
     4801 ccagtaaatt cccgttgaaa acagccttga aacttgcact gccattaaat gtagcatcta
     4861 aaatattcag tcctgtagta tatattcttg ttcgttccac gagcag
//
LOCUS       DAOOQP010000165         4902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20658_length_4902_cov_4.079018, whole genome shotgun sequence.
ACCESSION   DAOOQP010000165 DAOOQP010000000
VERSION     DAOOQP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20658_length_4902_cov_4.079018"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLK75_12145"
     CDS             complement(<1..989)
                     /locus_tag="PLK75_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOY40039.1"
                     /translation="MKTLTITGQGLTIENVVEVARLGRKVELSADALERIKKCRAMLE
                     RKIEAHEIMYGVNTGIGEFSEVVLNDDQVQQFQKYLIYNHAAGIGEAAPLEHVRAAML
                     GRINVHAHGNSGIRPEITLTLVDMLNKGVTPYVCQKGSVGACGDLAPMSQIALLMMGE
                     GKAYYNGELLEGKDAMMKAGIPIPGLKARDGLATINGSNVLTGMSALFLYDANRWLKQ
                     AEIACAMSLEALKANMRPYTALLHEVRGFKGAIRSALAIEKCVSGGDLKEGRVKCKVQ
                     DAYSMRSTPQVIGAAHDALAYARSQVETELNGVGDNPIFFPDKELALSGANFQG"
     gene            1124..1999
                     /locus_tag="PLK75_12150"
     CDS             1124..1999
                     /locus_tag="PLK75_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HOY40040.1"
                     /translation="MRKCLLLFTVFCLLSVALHAQCPDLTEDDVYSFEYDGHYYEIIK
                     KVYSWQNACACAVSRGGYLTAIESAGEQNAIWQAITNPASGVSLTYVQVADGGSIAYI
                     WIGASDIIAEGTWKWDGDNDGDGVIFYEGQGANGAGDGQPIEDRYNNWGGTFTGTNEE
                     PDNFVNIQDAAAIGLTSWPNGSANPLGIAGEWNDITAPDAIYSIVEYDDNPNSILNMN
                     SNSYMMPYFSAAENRIIVDVSAVHYKLYDITGRQIASGSISGKSVPIKQWNGVYLIEL
                     ADREAKVRVHKIYIY"
     gene            2878..4872
                     /locus_tag="PLK75_12155"
     CDS             2878..4872
                     /locus_tag="PLK75_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOY40041.1"
                     /translation="MKKLIILLSLALSFGSLLAQNTDLYNLLTEACRFNDYEMAKMLI
                     EEGADVNEKDYNGYTPLHIAVERESAIELVRLLIDKGADVNAAISENDTYWPGYTPVL
                     LTLQNGYLEIAKLLIENGADVNAKTSGESNSIYIDPAYTFLSGYSALHLALKNEYFDI
                     ARLLIEKGADANAKTSNNDFYYPGYTPLHFAIQAGNTDIAKLIIKKGADLSVRLPETS
                     QFPNASVFEFSCVFLDTEISRLLISKNYYESFTIDYEYNEFRGSSPLHLAVLMNDIEL
                     VALILDIDTCVSKQNSLGLTAGQLAKNAGFEEAYDYITKPDYKLFVFYHSGNTNGMKD
                     VLKKNKSLYGLTNANGETVWDLAVMDNNIEVLQLLLKNKKLVNTVNSRNQNVLFTAIL
                     KNNIHLALSIIESGIDCNHRDNMGMTPYFLSKNLNNDTISNALLTKNVDTSVHIVPKL
                     TVNFGEPYFIWAVAWSHDDRLIALGYDNGSIQIFEASTGRLIRSLDRQNGSVIFLSFT
                     SDDQRLISVTGVKPDRYFIRSEDNSFPFVNSNIVVWDINYGFMLNKTITEQIVAAEYL
                     SDKSCIATVDYSRNNIKVWSCPTLDLISIVAFNDSEIKSIDYSSDSGTLMAAGGNNIA
                     FIDATNFDVTYVTDSSCYYSHTIINQLSAVFDIFCMTVIT"
BASE COUNT         1443 a          964 c          995 g         1500 t
ORIGIN      
        1 ccttgaaagt ttgcaccgga taaagccagt tctttatcag ggaagaatat tggattatcg
       61 ccaacaccgt tcaattctgt ttcaacctgt gatcgtgcat aggcaagtgc atcgtgagcg
      121 gcaccaatca cctgtggtgt tgagcgcatt gaatacgcat cctgaacctt acattttacc
      181 cttccttctt taaggtcacc ccccgaaaca catttctcaa tggcaagtgc tgaacgaatg
      241 gctcctttaa atccccgaac ttcatgtagt agggcagtat agggacgcat gttggctttt
      301 aaggcttcga gcgacatggc acaggcaatt tcagcctgct tcagccagcg gttagcatca
      361 tataagaaca gtgcactcat tccggtgaga acattgctgc cattgattgt tgccagtccg
      421 tcacgggctt tgagtccggg gataggaata ccggctttca tcatagcatc ctttccttcg
      481 agtaattctc cattgtaata tgctttccct tcgcccatca tcagtagtgc aatctgcgac
      541 atgggagcga ggtcgccgca tgctcccaca gaacctttct gacaaacata gggggttact
      601 cctttattaa gcatatcaac caaggtcagg gttatctcag gccgaattcc tgaattccca
      661 tgggcatgca cgtttatacg cccaagcatt gcagcgcgaa catgttctag cggagcggct
      721 tcaccaatgc cggcagcatg gttataaatc aggtactttt gaaactgttg cacctgatca
      781 tcgttcagca ccacttccga aaattcacca attccggtat ttacaccata cataatttcg
      841 tgtgcttcaa tttttcgctc cagcatggcg cggcactttt taattctttc caaagcatca
      901 gccgataatt cgactttacg ccccaaacgg gcaacttcta caacattttc aatcgttaaa
      961 ccctgacctg taattgtgag tgttttcatg gattattatc ttattaattt ttgttataaa
     1021 attaatttaa tttcataatt caatacatca aaccgcaatt atttatttgt ttttaagagt
     1081 aatattttgt attttagaca ttaattaacc aaaccaatca attatgagaa aatgtttatt
     1141 attgtttact gtattttgtc ttctttctgt tgcattgcac gcacaatgcc ccgacttgac
     1201 cgaagatgac gtttatagtt ttgagtatga cggacattat tacgaaatta taaaaaaggt
     1261 gtattcatgg caaaacgcat gtgcctgtgc tgtgagcaga gggggctacc taacggctat
     1321 tgaatcagcc ggagaacaaa atgccatttg gcaggcaatc accaatccgg catccggagt
     1381 ttcgttgact tacgttcagg tggccgatgg tggaagtatt gcatatattt ggatcggagc
     1441 ctcagatatt attgctgaag gcacctggaa atgggatggt gataacgatg gggatggagt
     1501 gattttttat gaaggtcagg gtgcaaatgg tgccggagat ggtcagccaa ttgaagatcg
     1561 ttataataac tggggcggaa catttacagg gaccaatgaa gagcctgata attttgtgaa
     1621 catacaggac gcagcggcta ttggtctgac ttcctggccc aatggttcag cgaatccgtt
     1681 agggattgca ggggagtgga acgacattac agctcccgac gccatttatt caattgttga
     1741 atatgatgat aacccgaatt caatactgaa tatgaattca aattcgtata tgatgcctta
     1801 tttttctgct gcggaaaaca gaataatagt tgatgtttct gcagtacatt ataagctgta
     1861 tgatatcaca ggcagacaaa ttgcttcggg gagtatttca ggaaagtctg ttcccatcaa
     1921 acaatggaac ggtgtttatc tcattgaatt ggcagataga gaagccaaag ttcgggtaca
     1981 caaaatttac atatattaat accaggaggg tataaaaaac aaaaggggaa cttctgttcg
     2041 aaattcccct tcgctgttgg tttcccaacc gcggccggtt actgttatta aaccggtgca
     2101 aaaccttgcg gcgtcgcacc caggcgcgtc ttccttgcgg tttctgcgcc ctcatcggta
     2161 aaaacattcc gtttctcctc gttccgaaga ccgaggcttg ccggatcagc atcgttttgc
     2221 tgacttgttt agggcctgca atcgtgcttt cggatgcttg accgggaaca tgtatccata
     2281 tcccggcgca gtgcctgtaa gaacaacttc gcgaacctgc ttcgtgcggg ggttagcccg
     2341 cgtcggcaat gcccgttact cctgctcaac ggcgctgtcg gcaactgatt ccttgcggaa
     2401 ttttcattct gtgaaagaac ccggatgcgc ttcgctaaac ttgttagggt aaagtccccg
     2461 caccccttgt tttacagact ggctttttta tcgctcacct gctttacaaa aatatgctgc
     2521 cgataccccg tttgcaagtt tttttaggtt ttagtataaa catgttttta acgcatgatt
     2581 tcaacaaatt gttaataatt gacattatgt taaatgtcag tatatcagat aaatatttgc
     2641 tttatttgct aatagttgcg tttgactatc gatatgttaa taaagtttga cgtgtgtttt
     2701 taaacaactg cagagaaaaa ttattgcaac tgacgcgata attcagcacg attttcttca
     2761 taaaatagga atttacaggt gaaaaaagtc agaccaaaaa caaaaataac ttgcttttat
     2821 gtataataat ctttagattt gaatagtaga agatatacct gaacagaaaa ccaattcatg
     2881 aaaaaactta tcattttgtt aagccttgca ttaagctttg gcagcctatt ggcgcaaaac
     2941 accgacttgt acaaccttct caccgaagca tgtagattta acgactacga aatggcaaaa
     3001 atgctaattg aagaaggagc ggatgtaaat gaaaaggatt ataacgggta cacaccgcta
     3061 catattgcag ttgagagaga atctgcgata gaattagtcc ggctccttat tgacaaaggg
     3121 gctgatgtga atgccgcaat atcggaaaat gatacctatt ggccaggtta tacccccgta
     3181 cttttaacat tgcaaaatgg ttatttggaa atagccaaac ttcttatcga aaatggggca
     3241 gatgtgaatg ctaaaacatc gggtgagtcg aatagtattt atatcgatcc tgcgtacaca
     3301 tttttgtcgg gttactccgc attgcatttg gctttaaaaa atgagtattt tgatattgcc
     3361 agacttctta tcgaaaaagg agctgatgca aatgccaaaa catcgaataa cgatttttac
     3421 tatcccggat acactccatt gcattttgcc atccaagcag gtaatacaga tattgcaaaa
     3481 cttatcataa agaaaggtgc tgacctttct gtccggttgc ctgaaacctc tcagttcccg
     3541 aatgcttctg tttttgaatt ttcatgtgtt tttcttgata ccgaaatttc acgcttactt
     3601 atcagcaaaa attattatga aagttttacc atcgactatg aatataatga gttcagagga
     3661 agctctcctt tacatttggc cgttttaatg aacgatatcg aattggtagc attaattcta
     3721 gacattgaca cgtgtgtttc caagcagaat agtcttggac tcacagccgg tcaacttgca
     3781 aagaatgccg gttttgaaga ggcttatgat tatattacta aaccagatta caaattattt
     3841 gtgttttatc attcgggtaa tacaaacggt atgaaggatg tacttaaaaa aaataaatca
     3901 ttatatggtt taacaaatgc caatggagaa acagtctggg atctggctgt tatggacaac
     3961 aatattgagg tactccaatt attattaaaa aataaaaagc tggtaaatac tgtcaattcc
     4021 cggaatcaga acgtgttgtt tactgctatc ttaaaaaata acatccatct tgctttatca
     4081 attattgaat caggtatcga ttgtaatcat cgcgataata tgggaatgac accatatttt
     4141 ctttcaaaga atctcaataa cgatacaatt tcgaatgcgt tgctgacaaa gaatgtcgac
     4201 acatcagtac atatcgtacc caagctgact gtgaattttg gtgagccgta ttttatttgg
     4261 gctgttgcat ggtcgcatga tgataggtta attgcattag gatacgacaa tggatcaata
     4321 caaatttttg aagcatcaac aggtaggtta atacgttcgc ttgatcggca aaatggatcg
     4381 gtcatatttc tatcatttac ttctgatgat caacgattaa tttctgtcac gggcgttaaa
     4441 cctgataggt attttatcag atcggaagat aattcctttc catttgtaaa cagcaatata
     4501 gtggtttggg acattaatta tggttttatg ctaaataaaa ctataaccga gcaaatagtt
     4561 gcagcagagt atttaagcga taaatcatgc atagcaacgg ttgactatag cagaaataac
     4621 attaaagtat ggagttgccc aacattagac ttgatttcaa tagttgcttt taacgattcc
     4681 gaaattaaat caatcgatta ctcatccgat tcgggaactc tgatggctgc tggaggaaac
     4741 aacattgctt tcattgatgc aaccaacttt gatgttacct atgtaacaga tagttcatgt
     4801 tattacagtc acacaattat taatcagttg tcggccgttt tcgatatatt ttgtatgact
     4861 gtaataacat gaactatctg ttacataggt aacatcaaag tt
//
LOCUS       DAOOQP010000166         4890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20723_length_4890_cov_5.168769, whole genome shotgun sequence.
ACCESSION   DAOOQP010000166 DAOOQP010000000
VERSION     DAOOQP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20723_length_4890_cov_5.168769"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLK75_12160"
     CDS             <1..270
                     /locus_tag="PLK75_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40042.1"
                     /translation="FSKSGNANQEQLQPKSENKSVSVKRQAAEETMKEQKAVVQKSQV
                     KINEVKAKVNNTGTESKSNNNPELKREKTSQAEKRMKTMSDSGSK"
     regulatory      407..498
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            565..2973
                     /locus_tag="PLK75_12165"
     CDS             565..2973
                     /locus_tag="PLK75_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY40043.1"
                     /translation="MKRIALIFALGLFCVTPLFSQKLSGTVSDENGNPLRDVNIIIKG
                     SYSGTTTDENGAYTLQLKRGKNLLVFTYLGFESTEKEVNSYGSDAVLNVMLFTTAILT
                     DEVVVSATRAGSKTPTAYVNMDENEIGERNSAQDIPYLLELTPSFVATSEAGNGVGYT
                     GFRVRGTDATRINVTINGIPFNDAESQSIFWVNMPDFCSSVSSIQIQRGVGTSGNGSA
                     SFGASLNMETDFTEKKPYAGVQFIAGSFNTFKESLVLGTGIADNGMSFDVRMSKLNSD
                     GYINYSGSDHSSFLISGAWRNQSTLIRANVIYGNEKTGISWWGVPSDSLQTNRTYNPA
                     GEYYDETGTRQYYKNQTDNYVQTHYQVLLTKQLGKFFSINSALHYTRGDGYYEQYEQD
                     QMLSEYGFINDSTSDMIIRKWMGNNFYGIVYAANYQKGRINAKLGGACNQYDGNHFGK
                     IIWMRSAYNSEINHEYYRNTGNKTDLCNYLKLDFQLNEAINLYGDVQYRYILYRMAGS
                     DDDLMPDNTQKILDQQHIFNFFNPKAGVFYEINTKMNTYFSFALGHREPTRANFEDAV
                     GDENSTPKPETLFDYELGYRYSDKFISAGINIYFMDYTDQIIPTGQKSSVGYDIMTNV
                     RNSYRCGIELTGSLLFFERLKLNMNVTLSRNKIRNFVLYADQYDADWNASYIATPLGE
                     TDIAYSPTLIGAVGLSWNAFKNFVATWSCKFVGKQYFDNTMSNDRVIEPYCINNFQLD
                     YNIFSTKTKELSLRFSVNNIFNVTYSSNAYGGVWFEQGVEKTWAYFYPQAGINFSGGI
                     NIRF"
     gene            3026..3607
                     /gene="nrfH"
                     /locus_tag="PLK75_12170"
     CDS             3026..3607
                     /gene="nrfH"
                     /locus_tag="PLK75_12170"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOY40044.1"
                     /translation="MKLMRFIQPFFPNQRWKVPVIILIGVITGLGCNIIYVSKAWTYI
                     SDDPETCVNCHIMATEYTTWRHSSHGRNTNCNDCHVPHDNVFRKYYFKAMDGMRHSAI
                     FTTRGYEQSIRMLDPGKKVVLENCIRCHGPLTEMVKANVEFKDTKTGNGRLCWECHRE
                     VPHGRVKSLSSTPYTEVPYPKSPVPEWLRKITE"
     gene            3620..>4890
                     /locus_tag="PLK75_12175"
     CDS             3620..>4890
                     /locus_tag="PLK75_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOY40045.1"
                     /translation="MKFNLSERISKRPWIGWALFLVTVVVVFVLGLFASSIIQRRTEA
                     TVKNVPLYALDENEPRNEMWGKAYPREYATYQQMSDTSFSSKFNGNAMRDALDEDPRM
                     VILFAGYSFAKDYNQPRGHIYAITDVRNTLRTGTPLTDTDGPQPSSCWTCKSPDVLRV
                     MKNIGPEGFYKTMWAGMLSEIVNPIGCANCHNASDQSLRIAQIPFIEAYQRLGKNAGD
                     ATLNERRTLVCAQCHVEYYFKGDGKYVTFPWDKGFSVEDIEKYYDEIAFADWVHPLSK
                     TPIIKAQHPDFELFMSSVHFDKGVSCADCHMPYKTEGSQKITDHKIQSPLNNITNSCM
                     VCHRDSEEELMHDVYERQEKVYEQKILLEDLLVKAHIETAFAISKGATSEQLKAIHMY
                     IRQAQWRWDFVSASHGGSFHAPLECMRIISSG"
BASE COUNT         1529 a          943 c         1080 g         1338 t
ORIGIN      
        1 ttttctaagt caggaaatgc aaatcaggaa cagttgcaac caaaatcaga gaataaaagt
       61 gtttccgtaa aaagacaggc tgccgaggaa accatgaaag aacagaaagc tgtggttcag
      121 aagagccagg tgaaaattaa cgaggtaaaa gcaaaagtca acaacacggg aactgagtca
      181 aaaagtaata acaaccctga attgaaacgc gaaaagactt ctcaagccga aaagcgaatg
      241 aagacaatgt cggattccgg cagtaaataa aaatattttt caatatttaa aataacagcg
      301 taaaggagta gttgcaatat attgctcctt tcgttgttat aaaacattgg atactcaaca
      361 tttcagtttt tagaaaagct aaagtatttt tatctttacc accgataaat cttgatgggg
      421 gtgcctgaag cggctgagat taaaccctta taacctgatc cgggtaatgc cggcgtaggg
      481 aattaagcaa ctcattttac cgtttcatac atcagctaat cagtttatta gatttaattt
      541 attgaaattt tagtttaact gaatatgaaa agaattgcct taatttttgc cctaggctta
      601 ttctgtgtaa cgcccttgtt ttcgcagaag ctgagcggta ctgtgagcga tgaaaacgga
      661 aacccgttgc gcgatgtgaa cattattatt aaagggagtt attcaggaac aacaaccgat
      721 gaaaatggag cttacaccct gcagctgaaa cgtggcaaaa acctattggt ttttacttac
      781 cttggttttg agagcactga aaaggaggtg aacagctatg gcagcgatgc tgtgctcaat
      841 gttatgctat tcacaacagc tattttgaca gatgaagtag tggtaagtgc aacgcgtgca
      901 ggatcgaaaa ccccgacagc ctatgtaaat atggacgaaa acgaaatcgg tgaacgaaat
      961 tcagcgcagg atattccgta cttactggag cttactccat cttttgttgc tacaagcgag
     1021 gctggtaatg gagtagggta tacggggttc cgtgtcaggg gaacggatgc aacccgtata
     1081 aacgtcacta tcaacggcat accattcaat gatgccgaat cgcaaagcat tttctgggta
     1141 aatatgccag atttctgttc atcggtaagc agtattcaga ttcaacgggg cgttggaaca
     1201 tcgggtaatg gttcagcatc attcggggca agcctgaata tggaaaccga ttttaccgaa
     1261 aaaaagccct atgccggcgt tcaattcatt gcaggcagtt ttaatacctt taaagaaagt
     1321 cttgttttag gaacgggtat tgccgacaat ggaatgtcgt ttgatgtaag gatgtcgaag
     1381 cttaacagcg atgggtatat caattattcc ggctcagacc acagctcatt tctgatatct
     1441 ggagcctggc gcaatcaatc gactctgata cgggccaatg ttatttacgg taacgaaaaa
     1501 accgggatca gttggtgggg agtgccctca gacagccttc agacaaaccg tacatataac
     1561 cctgccggag aatattacga tgaaacagga acccgacaat actataaaaa ccaaaccgat
     1621 aattacgttc aaacgcatta tcaggtgctg cttacaaagc aattgggaaa atttttcagc
     1681 attaattcag ccttgcatta cacgcgtggc gacgggtact acgaacagta tgaacaagat
     1741 cagatgcttt cggaatatgg gtttataaac gattcaacga gcgatatgat tatccgtaag
     1801 tggatgggaa ataattttta tggaattgtt tacgctgcca actatcagaa aggacgcatc
     1861 aatgccaaac ttggaggagc ttgcaaccag tacgatggta atcatttcgg aaagattatc
     1921 tggatgcggt cagcttacaa ttcagagata aaccatgaat actaccgcaa tacagggaat
     1981 aaaacagacc tatgcaatta tttaaaactc gattttcagt taaacgaagc tatcaattta
     2041 tatggcgatg ttcagtacag gtatatcctt taccggatgg ctggtagcga tgatgatctt
     2101 atgcccgaca atacccagaa aatactcgac cagcagcata tcttcaattt ctttaacccc
     2161 aaagccggtg tattttatga aatcaacaca aagatgaata cgtacttctc attcgcattg
     2221 ggccatcgtg agcctactcg ggcaaacttc gaagatgcag ttggcgatga aaacagtaca
     2281 cctaaaccgg aaacactgtt cgattatgag ctgggataca ggtattctga taaatttatt
     2341 tcagctggta tcaatatata tttcatggat tacacagatc agatcattcc tacaggtcaa
     2401 aagagctcag tcggttacga tattatgacc aatgtaagaa acagctaccg ttgtgggatt
     2461 gaattaacag gtagtttatt attttttgaa cgccttaaac taaacatgaa tgttacgctg
     2521 agccggaata aaatcaggaa ttttgtgctg tatgccgatc agtatgatgc cgactggaac
     2581 gcgtcgtaca ttgcaactcc attgggtgaa actgatatag catattctcc aacattaatt
     2641 ggagctgtag gcttgtcgtg gaatgcgttt aaaaattttg tagctacatg gtcatgtaag
     2701 tttgtgggta agcagtattt cgacaatacg atgagtaacg accgggttat cgaaccctat
     2761 tgtattaaca attttcaact cgattacaat atattctcaa caaaaacaaa ggagctgtca
     2821 ttacgatttt cggttaacaa tatttttaat gttacctatt catcaaatgc atatggtggc
     2881 gtttggtttg agcagggtgt tgagaaaaca tgggcatatt tttatccgca ggcaggaatc
     2941 aacttttcgg gtggtataaa tatcaggttc tgataaaaac tcttgattgc tttgaatatt
     3001 tagttataat actatctttg tattaatgaa attgatgcga tttattcagc ctttctttcc
     3061 caatcagcga tggaaagtgc ctgtgataat actgataggg gttatcactg gtttgggttg
     3121 taatattatc tacgtatcca aagcgtggac gtacatttcg gatgaccctg aaacctgcgt
     3181 gaattgccat atcatggcaa cagaatatac aacatggagg cattcttctc atggcaggaa
     3241 caccaattgt aacgactgtc acgttccaca tgataatgtt ttcagaaaat actatttcaa
     3301 agcaatggac gggatgcggc attctgcaat tttcactacg cggggctatg agcaaagtat
     3361 tcgtatgctt gatcccggca aaaaagtggt gctcgaaaac tgtatccgtt gtcatgggcc
     3421 tctcaccgaa atggtgaaag ccaatgtgga gttcaaagat acaaaaacag gcaatggtag
     3481 gttgtgctgg gaatgccaca gagaagtacc tcatggcagg gtgaaaagcc tttcatctac
     3541 cccatatacc gaggtgccat atccaaaatc gcctgtcccc gaatggttgc gaaaaattac
     3601 tgaataaaac aattttgata tgaaattcaa cttatccgaa agaatcagca aaaggccttg
     3661 gattggctgg gcactttttt tagtaacagt ggttgtcgta tttgttctgg gtttgtttgc
     3721 ttcatcaata attcaacgca gaactgaagc tacagtgaaa aatgtgccat tatatgctct
     3781 cgacgaaaat gaaccccgca atgaaatgtg gggtaaggct tatccgcgcg agtatgccac
     3841 ctaccaacag atgtcagaca cttctttctc ttcaaaattc aatggaaatg ccatgcgcga
     3901 tgccctcgat gaagacccac gtatggtgat tctatttgcc ggatactcat ttgcaaagga
     3961 ttacaatcaa cctagaggtc acatttacgc aattaccgat gtgagaaata ctcttcgcac
     4021 agggacgccc cttaccgata ccgatggacc acaaccatcc agctgctgga cgtgtaaaag
     4081 ccctgatgta cttcgtgtta tgaaaaatat cggacctgaa ggcttctaca aaaccatgtg
     4141 ggcaggaatg ctgagcgaga tagtaaatcc gattggttgc gccaattgcc ataatgcctc
     4201 cgatcagagc ctacgtatag ctcagattcc ctttattgaa gcctatcaga ggttgggtaa
     4261 aaatgccggt gacgctactt tgaatgagcg ccgaactttg gtgtgtgcac aatgtcatgt
     4321 ggaatattat ttcaagggag acggtaaata tgtaactttt ccgtgggata agggatttag
     4381 cgtagaggat atcgaaaaat attatgatga aattgctttc gccgattggg ttcatccctt
     4441 gagtaaaacg cccattatta aagcacagca cccagatttt gagctgttta tgtcgagtgt
     4501 gcattttgat aaaggggttt catgtgccga ttgccatatg ccttataaaa ccgaaggcag
     4561 tcagaaaatt accgatcaca aaattcaaag cccactcaat aatatcacca attcgtgtat
     4621 ggtttgccac cgcgacagcg aagaagaact catgcatgat gtgtatgaac ggcaggagaa
     4681 agtgtatgaa caaaagattt tgcttgaaga cctgctcgta aaagcacata ttgagacagc
     4741 atttgccatt tctaaaggcg caacttcaga acagctaaag gcaattcata tgtacatcag
     4801 gcaggcacaa tggaggtggg actttgtttc agcaagtcat ggcggctcgt tccatgctcc
     4861 cctggaatgc atgagaataa tttcgtcggg
//
LOCUS       DAOOQP010000167         4881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20756_length_4881_cov_5.173850, whole genome shotgun sequence.
ACCESSION   DAOOQP010000167 DAOOQP010000000
VERSION     DAOOQP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20756_length_4881_cov_5.173850"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLK75_12180"
     CDS             <1..329
                     /locus_tag="PLK75_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40046.1"
                     /translation="TVPKTGLQNSFELMRLMFADLRKGTGAVVISAAAGGGYALENED
                     IENGIFTYSLIQAFKKNKADVNRDGYISVSELRNYIFDKVNELSGGRQQPTSRKENLN
                     NDFTIW"
     gene            412..3729
                     /locus_tag="PLK75_12185"
     CDS             412..3729
                     /locus_tag="PLK75_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY40047.1"
                     /translation="MNAQKITLPISNVSAVMIFATMFCVAINTNCLSQVDVQNKTQVA
                     VVLPVGHTEYVKSCEFSSDGRFILTASLDKNIILWDVKTGYELKRYQGSPLGVMKAVF
                     SPDNRYIASVGWDLKIRLWNLETAALINTFGEGMNCVIFSKDGSKLYADSRDGRVGIY
                     NSASGEKTGEIKAHTGIINDMSVSPDGSRLFTVSADGSCKVWALPAMKLIHTFSIEKG
                     GIQDAVCGKSGLIAYGCSDGVVKLCNSDNFSQIREFKAHEASVVDIDFSADGKLLSTT
                     GWDGKLKIWDTFSAELISSVDAHKFLVYDAAFSPVGKYIATCGTDKDVKLWDVESGTL
                     LRYYTGHTTKVASIDADTDEKVMVAGLWDQIRFDDNRPVFWNLGNGSLEASGGSHSFM
                     LNAVALSAETEIAASVAEDTRVMLWNTHNGSLINENRIPQGGLKDVCFYNTEQKIAVA
                     CTDKTVRVLTQQGMELYQVLPCENKDPSCVDVDKNDRLLAAGFTDGTVMVWNAETGKL
                     ITEFAAHKDPVYDIKFSPGSDHLVTASYDKTAVVWSLPDFKKVSEFKGHEWIVSSIAI
                     AGDGTIVFTGSWDRQVCQWNMFTGELIRKYSGHSNYVSGITLSSNGKQLFSCSWDGSI
                     IAWDTQTGKKICTFISVDDNDWLVVTPDNFYMGSKDAARKVSFTSGMNSFGFEQFDLQ
                     YNRPDKVLEQLPWADSALIPVYRKAYEKRLIKSGVNESWFGPDFHSPEIRILNRSEIS
                     LITENNAIELHLILSDSVYKINTLNLWVNGVPYYGVSGKSVEGIADLKNYRVTIPLSY
                     GKNRIEAACMNEKGVFSLKDEIEIYQENNNPRKPDLYLISLAVSEFDDTRYNLKYTVQ
                     DGRSFIKCFSPPNNRFGHVYIDSLYNENCTRENLRNIRQKLMNTHVDDEVIVHVAGHG
                     LLDDKQDFYFAVSNINFKNPSVNGINYAEIESLLDSIPARNKLLLLDACHSGEVDEAS
                     EMAPVLTDNARTRGVVMYKLPVSELSTNDINLKNSFDLMREIFVDVRRSTGAVVISAA
                     SGAGFAIEREDLQNGIFTYCIVEAIEDLRADKNNDGSISVSELRDFVIKRVREISDGQ
                     QFPTSRKENLINDFRVW"
     gene            4221..4766
                     /locus_tag="PLK75_12190"
     CDS             4221..4766
                     /locus_tag="PLK75_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40048.1"
                     /translation="MNLYNSVAPPVIRVINALFKPLLLSFILLIGNAQTNAQESPGYT
                     GNSQNRIAPHAPDYNDPNYEVKKNEWIANYPEEYNALQPNGNQNTIVDVSLIPEASLK
                     APLLGPHAPDSDDPDFEAKKAEWIKNYPEEYNVLHRNSVITCNEAPQLITPSEIDNQL
                     GPLIELPASDIDPECVDNEDE"
BASE COUNT         1505 a          869 c         1093 g         1414 t
ORIGIN      
        1 atacggtacc caagacaggg ctgcaaaact cattcgagtt gatgcgcctc atgtttgccg
       61 atttacgtaa aggtacaggc gcagtggtta tttctgctgc tgccggtggc gggtatgccc
      121 tcgaaaatga ggatatcgag aacggtattt ttacctattc acttattcag gcatttaaga
      181 aaaataaagc agatgttaac agagatggtt atatctcggt ttctgaactc cgtaattata
      241 ttttcgataa ggttaacgag ctcagtggcg gacgtcagca acctacttcg cgcaaagaaa
      301 acctgaataa cgatttcaca atttggtagt tctttgtgtc aggcctgtaa tgatttacta
      361 cagttcattc gacttttaaa ataggtgttt tttactataa gtgttattcc tatgaatgcg
      421 cagaaaatta ctcttcccat ttcaaatgtg tcggcggtga tgatttttgc aacgatgttt
      481 tgcgttgcaa taaacaccaa ttgtttgtcg caggttgatg ttcagaataa aacacaggtt
      541 gcggtagtgc tccctgtcgg gcatactgaa tatgtaaaat cctgtgagtt ctcgtccgat
      601 ggcaggttta ttcttactgc ctctctcgat aaaaacatta ttctatggga tgtgaaaaca
      661 gggtatgaat taaagcgata tcagggatca cctcttggtg ttatgaaagc agtattttcg
      721 cccgataacc ggtatattgc cagtgtggga tgggacctta aaatacgact ttggaacctt
      781 gaaacggcag ctcttatcaa tactttcgga gaaggtatga attgcgtgat attttcgaaa
      841 gatggcagta agctatatgc tgatagccgc gatggaagag ttggcatcta caactcggcc
      901 agtggtgaaa aaaccggcga gataaaagcg cataccggaa ttattaacga tatgtcggtt
      961 tctcccgatg gcagccgatt gtttactgtt tcggctgatg gatcatgtaa ggtttgggcg
     1021 ctacctgcca tgaagctgat tcatacattt tcaatagaga agggcggcat tcaggatgcg
     1081 gtgtgtggaa aatcagggtt gatagcttac ggatgcagcg atggagtagt aaaattatgt
     1141 aatagtgata atttttctca aattagagag ttcaaggctc atgaagcaag tgtggttgat
     1201 atagatttct cagccgatgg caagctattg agcactaccg gttgggacgg aaaactgaaa
     1261 atctgggaca cattttcagc agagcttatt tcgtcggtcg atgcgcataa atttttagtt
     1321 tatgatgcag cattttctcc cgttggaaaa tatattgcta cctgtggcac cgataaggat
     1381 gttaaacttt gggatgttga atcgggaacc ctcttgcgtt attacacagg gcatacaaca
     1441 aaagtggctt caatcgacgc tgacaccgac gaaaaggtta tggtagcagg attgtgggac
     1501 caaatacgct tcgatgataa ccgaccagtg ttttggaatt tgggaaacgg ttctcttgaa
     1561 gcttccggtg gttcacattc atttatgctg aacgctgttg cgctgtcggc agaaaccgag
     1621 attgcagctt cggttgccga agatacacgg gtaatgcttt ggaataccca taatggcagt
     1681 ttaattaacg aaaatcgcat cccgcaaggc ggattaaaag atgtttgctt ctataataca
     1741 gagcaaaaaa tagcggtagc atgcaccgat aaaaccgtgc gggttttaac ccagcagggt
     1801 atggaacttt accaggtatt accttgcgaa aacaaggacc caagttgtgt tgatgtggat
     1861 aaaaacgatc gtttgcttgc ggctggtttt accgatggaa ccgtaatggt ttggaatgct
     1921 gaaactggca aattgattac tgaatttgct gctcataaag atcctgttta tgacattaaa
     1981 ttcagcccgg gtagcgatca tttggttact gcttcttacg ataaaaccgc tgtggtttgg
     2041 agtctgcccg attttaaaaa agtgtcagaa tttaaaggac acgaatggat tgtgtcatcg
     2101 atagccatcg cgggtgatgg aacaattgta ttcaccggat cctgggacag gcaggtttgt
     2161 caatggaaca tgtttacagg agagctaatt cgtaaatact ccggtcacag taattacgtt
     2221 tcagggataa cactcagcag taacggaaag caactttttt catgttcttg ggacggaagt
     2281 attattgcat gggatactca aacaggaaaa aaaatatgca catttatcag tgtcgatgat
     2341 aacgactggt tggtagttac ccccgataat ttctacatgg ggagcaagga cgcagcgcgc
     2401 aaggtatcgt ttacatcggg gatgaacagc tttggttttg aacagtttga tctgcagtat
     2461 aaccgacctg ataaagttct cgaacaattg ccatgggccg attctgcttt aataccggtg
     2521 tatcgtaaag cttacgaaaa gcgactgatt aaatcagggg ttaacgaaag ctggttcggc
     2581 cccgactttc atagccctga aatcaggata ctcaaccgca gcgaaatttc gctgataact
     2641 gaaaataatg caattgagct ccatcttatt ttatccgaca gcgtgtataa aattaatacg
     2701 ctgaacctat gggtgaacgg tgtaccttac tatggtgtgt caggaaagag tgtagaaggg
     2761 atagctgacc ttaaaaatta cagggttacc atcccgctaa gctatggaaa aaacaggata
     2821 gaggctgcgt gtatgaacga aaaaggagtc ttttcactga aggatgaaat cgaaatatat
     2881 caggaaaaca ataatcccag aaagcccgac ttatacctga tatcgcttgc agtttcggaa
     2941 tttgatgata caagatataa tttgaaatat acagttcagg atggaagaag ctttattaaa
     3001 tgtttttctc ctccaaacaa tcgctttggg catgtatata tcgattcgct gtataacgaa
     3061 aactgtaccc gcgaaaatct gcgtaatatc cgacaaaaac tgatgaatac tcatgtcgat
     3121 gatgaagtta ttgtgcatgt ggcagggcat ggcttactcg acgacaagca ggatttttat
     3181 tttgccgtaa gcaatatcaa tttcaaaaac ccttcagtca atggaatcaa ctatgccgaa
     3241 atagagagct tactggatag cattccggca agaaataagc tgttgctgct tgatgcatgc
     3301 catagcggcg aagttgacga ggcttccgaa atggcaccgg ttcttaccga taatgcccgc
     3361 acaaggggag tggtgatgta taaattgcct gtttctgaat taagcacaaa cgatatcaac
     3421 ctgaaaaatt cattcgacct gatgagggaa atatttgttg atgtgcggcg cagtaccggg
     3481 gctgtggtaa tttcagctgc cagtggagca ggttttgcta tagaacgtga ggacctgcaa
     3541 aacggaattt ttacctattg cattgtagaa gctatcgaag atttacgtgc tgacaaaaac
     3601 aatgatggca gtataagcgt gagtgagttg cgcgattttg tgataaaaag agtcagagaa
     3661 atcagcgatg gacagcagtt tccaacatcc cgcaaagaaa acctcattaa tgacttcaga
     3721 gtttggtaaa acctaaaaga caaatgccaa atgccaaaag acaaatgtca aaagtaatgg
     3781 attcagagtc gggtaaaaga atatttttgc tcagccaagg caatagatat ttatcgaaaa
     3841 aatacccaga aataccttta acatatttaa aataagtaat aaattctgat ggtattcata
     3901 tgtcttaaaa catgatgaat gtataagtaa ttgtgcaaca tggttttttt tgattttgag
     3961 tattctgatg aattcttaag gtgataggat tgtataactt gtaaattatt gaaattaatt
     4021 tatataatat gataatatat atattctaaa atcatgagat ggtaatgctg acaatgagag
     4081 gtaaccaacg gtctattggt ttataaaaag tatgtataaa aaacatataa atcgtcgggt
     4141 gactttttat ataatgatac ttttgtgttc cttttagttg tgtcagttac atttggtatt
     4201 aatgataatt tttcgttaag atgaatttat acaattctgt tgctcctcca gttattcggg
     4261 tgataaatgc tttgtttaaa ccattgctgc tttcattcat tctacttatt ggtaatgctc
     4321 aaactaatgc tcaggaaagc cccggataca ccggaaattc tcaaaaccgc attgcaccgc
     4381 atgctcccga ttacaatgac cccaattatg aagtcaagaa aaatgaatgg atagcaaatt
     4441 atcctgaaga atacaacgca ttgcaaccca atggcaatca aaatacgata gttgatgtgt
     4501 cgcttatacc cgaagcttcg cttaaagctc ctttgcttgg accccatgct cccgattcag
     4561 atgatccgga ttttgaagca aagaaggcag aatggattaa gaattaccct gaagagtata
     4621 atgtgttgca tagaaattct gtaataactt gtaatgaggc accacaacta ataactcctt
     4681 cagaaattga taaccaacta ggtccgctga ttgaattacc ggcttcagat atagaccctg
     4741 aatgtgttga taatgaagat gagtgagtaa aagtccatca ttaaatttat taataaagag
     4801 cgaaggggtg taaagaccta tagaaataaa ctactcccca ttctttagac cacttttttg
     4861 tattcttaat tgaaatctct a
//
LOCUS       DAOOQP010000168         4853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20895_length_4853_cov_3.135682, whole genome shotgun sequence.
ACCESSION   DAOOQP010000168 DAOOQP010000000
VERSION     DAOOQP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20895_length_4853_cov_3.135682"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2219
                     /locus_tag="PLK75_12195"
     CDS             <1..2219
                     /locus_tag="PLK75_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY40049.1"
                     /translation="SPTTGLNNPASLNPVATPSATTTYTITVQDNTGCTASDNVTITL
                     YPAATVNLGPDVQVCSSALPYTIDAGAGYTNYDWSVNAYDGSQTMPVSASGNYSVTVT
                     NSNGCTATDNINVTVVSNPVVNLGIDQTVCSEDLPFTLNAGPGFAVYDWNIDAYDGSQ
                     TMPVNTTGSYSVTVSDGSGCTGSDQISITVDPLMNITLVSVNPLCFGTATGSITANVT
                     GGTPAYQYYWSNGQTTPSISGLSAGVTYAVTVTDNIGCSVTASAVLTSPPQLVATVTT
                     QPTECGQAIGSATVSASGGAGGYTNLWNTSGNSDQIINLIPGTYSCTVTDANGCSVVD
                     SEVVGIYGSGDVVITLLQPVLCNGFTTGVLQGEMTDGTAPFQYSWLGLPQHTAVVNNL
                     PAGNYNITITDTYGCTGTASYTITEPPAIAATSEIVDVLCRGGNNGAITLTVTGGVSP
                     YVFQWSGGQTSSHISGLTAASYSVVITDMNNCTYQGFYTIEQPEKEMDLTLLTQDVIC
                     YNQNTGAAIANGDGGTPPYVVIWFSGGVAVGNGSEINSLPAGTYEVLVRDANNCEARR
                     FFTITEPAEISLEYSVTAATCMGNDDGQALISVIGGTQPYSFEWNTGDMLPLLEDATA
                     GQYVVSVTDANECTKMLNIYIPESNDLCLRIPNAFTPNDDGINDTWVIDYIDKYPLAQ
                     VYVFNRWGQKMYDKGNNQGPWDGKLEGKKVPTGSYTYIVDLRNGMEPFTGVVVVVH"
     gene            2344..2772
                     /locus_tag="PLK75_12200"
     CDS             2344..2772
                     /locus_tag="PLK75_12200"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOY40050.1"
                     /translation="MLVAKAGSIEHSGVVHEISEGTIKVKILVSSACSSCRANTMCGS
                     SSGEKIIEINQFDEPYSIGEEVMLRMHESLGFKAIVWGYLLPFVLVMICLIGLLLITG
                     NEALSGIISLIVLVPYYLVIFLLRDRFKRVFRFEISKIIN"
     gene            2779..3648
                     /locus_tag="PLK75_12205"
     CDS             2779..3648
                     /locus_tag="PLK75_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOY40051.1"
                     /translation="MNLILFTVITLLVLSAFLAIILYLVAQKFKVVEDPRIDEVEACL
                     PGANCGGCGSPGCRGFAEACVKADSLGSLFCPAGGNAAMAKIAQVLGQVAVEKDPMVA
                     VVRCAGSFEHRPKTNTYDSVKSCAVASMLYSGDTGCPNGCLGLGDCVAVCSFDAIYID
                     KNTGLPVVSDQKCTACGACVKACPKNIIELRLKGKKDRRIFVSCINTEKGGVAKKSCT
                     VACIGCGICMKNCAYDAIKIENNLAYIDFEKCKLCRKCVEVCPTNAIHELNFPPRKQK
                     AEENAISGETVNN"
     gene            3678..>4853
                     /gene="rsxC"
                     /locus_tag="PLK75_12210"
     CDS             3678..>4853
                     /gene="rsxC"
                     /locus_tag="PLK75_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOY40052.1"
                     /translation="MLKTFPKGGVHPAENKLSEGKNIIKLEIPKLVNIPVSQHLGAPA
                     QTLVNKGDTVKAGQIIAKPGGFVSTNIHASVSGIVTKVDKVLDSSGYKQTCITIETSG
                     DEWVEGVDLSDTLIRDIKLTDTEIRNKLLESGIVGMGGAGFPTHVKLTVPQGKQANYL
                     IINAVECEPYLTSDHQLMMEKAEEILVGALIMKKAIGAEKVIFGIENNKPNAIQHLKK
                     LASSYTDIEIQGLKVKYPQGGEKQLIRALVKREVPSGGLPIDVGCVVQNVGTAFAVYE
                     AIQKNKPLVERVVSVTGKSVMNPINVKARIGTPVDMLIEAAGGLPDDTAKVVNGGPMM
                     GKALSDTSVPVTKTTSGILILNDKSAKRPAEFSCIRCARCTHVFPMGLEPYLIKNMVL
                     "
BASE COUNT         1398 a          957 c         1126 g         1372 t
ORIGIN      
        1 ggtcgcccac aaccggacta aacaaccccg cctcactgaa ccccgttgcc acaccatcag
       61 caactaccac ctataccata acagtgcagg ataatacggg ttgcactgct tccgataatg
      121 taacaataac cctttaccct gcagctaccg tgaatcttgg tcccgatgtg caggtttgct
      181 cctcagcttt gccgtatacc atcgacgccg gtgcaggcta tactaactac gactggagcg
      241 ttaacgccta tgacggcagc cagactatgc cggtttctgc atccgggaat tattctgtta
      301 cggtgacgaa ttcaaatgga tgcactgcga ctgataatat taatgttact gtggtttcta
      361 atccggtggt aaatttgggt attgaccaga cggtatgttc cgaagacctt ccgtttacct
      421 tgaatgcagg tccgggattt gctgtttacg attggaatat tgatgcctac gacggaagtc
      481 agacaatgcc ggttaacacc accggcagct attccgtaac agtatccgat ggtagcggat
      541 gtactggttc cgaccaaata tcaattactg ttgacccact catgaatatt acactggttt
      601 cggtaaaccc tttgtgtttt ggaactgcta ccggctcaat aactgctaat gttacaggag
      661 gtactccggc atatcagtat tactggagca acgggcaaac cactccttca atcagcggat
      721 taagtgcagg ggttacttat gctgttacgg ttaccgataa cattggctgc tccgtaactg
      781 catcagcagt attgacttca ccaccgcagc ttgttgcaac tgtaactaca caacctactg
      841 aatgcggcca ggcaattggt agtgctacgg tttctgcatc aggaggagct ggaggttata
      901 ccaatttatg gaacacttcc ggaaattcag atcaaattat taacctgatt ccaggaacct
      961 attcttgcac tgtaactgat gcaaacggat gctctgtggt tgattctgag gttgttggaa
     1021 tatacggctc aggcgatgtt gtaatcactc tgttgcaacc tgtgctgtgt aatggcttta
     1081 ccacaggagt actgcaaggg gaaatgactg acggaacggc tccattccag tatagctggc
     1141 ttggactacc acaacatact gcggtggtaa ataatttacc ggcaggcaac tacaatataa
     1201 ccattaccga tacttacgga tgtacaggta cagcaagtta tacaattacc gaaccccctg
     1261 caatagcagc aacatccgaa attgttgatg tgctttgtcg tggaggtaat aatggggcaa
     1321 ttacgcttac cgttacgggt ggagtgtcgc catacgtatt tcagtggtca ggggggcaga
     1381 cttcaagcca tatatccgga ctcacagcag cttcttacag tgttgtaatt actgatatga
     1441 ataattgcac ttatcagggc ttctacacta tagaacaacc tgagaaagaa atggatctta
     1501 cgctactaac tcaggatgtg atttgctata atcagaacac aggagctgca atagccaatg
     1561 gagacggggg aacccctcct tatgtggtta tttggttttc gggcggtgtg gcagttggaa
     1621 acggatcaga aataaactcc ttacctgcag gtacttatga ggttcttgtg cgcgatgcca
     1681 ataattgcga agcgagaaga ttttttacca taactgagcc tgcggaaatc agccttgagt
     1741 atagtgttac agcggctacc tgcatgggta acgatgacgg acaagctctc atttcggtta
     1801 ttggcggtac acaaccctat tctttcgaat ggaatacagg agatatgctt cctttactgg
     1861 aagatgccac agccggacag tatgttgttt ctgttaccga tgccaatgaa tgtacaaaaa
     1921 tgctgaatat ctatattcct gaatcaaacg acctctgtct gagaatacca aatgcattca
     1981 ctcccaatga tgacggcatc aacgatacct gggtaattga ttatatcgat aagtaccccc
     2041 ttgcacaggt ttatgttttc aaccgctggg ggcagaaaat gtatgacaag ggtaataatc
     2101 agggcccctg ggatgggaaa ctggaaggca agaaagttcc taccggatca tatacttaca
     2161 ttgtagattt acgcaacgga atggagcctt ttacaggagt tgttgtagtg gtgcattaat
     2221 tgccagcgtg ttctcatacg ccttgtttaa aacgatttta aattagtaaa atcgcgtact
     2281 tttcatttaa aaacatatat tattacttgg aaataatttt acctttgaca tcttaaaatt
     2341 ttgatgttag tggcaaaagc cggaagcata gagcatagcg gagttgttca tgaaatatcg
     2401 gaaggaacaa tcaaggtaaa aatacttgtt tcttcagctt gttcgtcttg cagagccaat
     2461 acaatgtgtg gttcaagttc cggtgaaaag ataatagaga tcaatcagtt cgatgaacct
     2521 tactcaattg gtgaggaagt aatgcttcgc atgcacgagt cactgggttt taaagccatt
     2581 gtatggggat atctgttgcc ttttgtacta gtaatgattt gtttaatcgg gttgcttctg
     2641 attacaggaa atgaagcact ttccggaatt atttcactga ttgttctggt gccatattat
     2701 ttagtaatat ttctgttgag agatcggttt aaaagagttt ttcgttttga aattagtaag
     2761 attataaatt gatttgttat gaatctgatt ttgtttactg ttatcaccct attggttcta
     2821 agtgcatttc tggcaataat tctatatttg gttgcccaga aatttaaagt tgttgaagac
     2881 ccgaggattg atgaagttga agcatgcctt ccgggcgcta attgcggtgg ttgtggttct
     2941 ccaggatgcc gaggttttgc tgaagcttgt gtgaaagctg attcccttgg gtcgttgttt
     3001 tgccctgccg gaggcaatgc agccatggca aaaattgctc aggtgctcgg gcaagtagct
     3061 gttgaaaaag atcctatggt tgctgttgtt cgttgtgcag gtagttttga acacaggccg
     3121 aaaaccaata catacgacag cgttaaatca tgtgctgttg cttctatgct ctatagcggt
     3181 gacacaggat gcccaaacgg ttgccttggt ttgggcgatt gtgtggctgt atgctcattc
     3241 gatgctattt atatcgataa aaatacagga ctgccggttg tatccgacca gaaatgtact
     3301 gcatgtggtg cttgtgtgaa ggcttgtccg aaaaatataa tagaactgcg tcttaaaggg
     3361 aaaaaagacc ggaggatatt tgtatcatgc attaacactg aaaagggggg tgttgctaaa
     3421 aaaagctgca ccgtagcctg cattggttgt ggaatctgta tgaaaaactg tgcttacgac
     3481 gctattaaaa ttgaaaacaa tcttgcatat atcgattttg agaaatgtaa attgtgtcga
     3541 aaatgcgttg aagtatgtcc gactaacgca attcatgaac tgaatttccc accccggaaa
     3601 caaaaagcgg aagaaaacgc aatatcaggg gaaacggtaa acaattaata actaactctt
     3661 aaacgtaagg aggtaacgtg ttaaagactt ttccaaaagg cggtgtgcat ccggcagaaa
     3721 ataagttgtc agaagggaaa aatattatta aacttgaaat tccaaaactg gttaatatcc
     3781 ctgtttcgca acatcttggt gctcctgcac aaacattggt caataaaggt gataccgtaa
     3841 aggcgggtca gattattgcc aaacccggtg gctttgtttc aacaaatatt catgcttctg
     3901 tatcaggtat cgtgaccaaa gtcgataagg ttcttgatag ttcgggatac aaacaaactt
     3961 gtatcactat tgagacatca ggtgacgaat gggttgaggg tgttgatctg agcgatacct
     4021 taatcagaga tattaaactc accgatacgg agattcgtaa caagcttctc gaaagcggaa
     4081 ttgttggtat gggtggagca ggttttccaa cccatgttaa gttaaccgtg ccacagggaa
     4141 aacaggcaaa ttatctgatt atcaatgctg ttgaatgtga gccttacctt acttccgacc
     4201 atcagcttat gatggaaaaa gcagaagaaa ttcttgtagg cgctttgata atgaaaaaag
     4261 ccattggagc agaaaaagta attttcggta tcgaaaacaa taaacctaat gcaatacagc
     4321 atcttaaaaa actagcttca agctatactg atattgaaat tcagggtttg aaagtaaaat
     4381 atccacaggg aggtgagaaa caacttataa gggcgctggt taaacgtgaa gttccttcag
     4441 gaggattgcc tatcgacgtg ggctgcgtag tgcaaaatgt tggaaccgcc tttgcagtat
     4501 acgaagccat acaaaagaac aaacctttgg tagaacgtgt tgtttctgta accgggaaat
     4561 cggtaatgaa tccgataaat gtcaaagccc ggattggtac tccggttgat atgttaattg
     4621 aagccgcagg tggcttaccc gacgatacag ccaaagtagt taacggtggc ccgatgatgg
     4681 gtaaagcgct tagcgacact tctgtccctg taaccaagac aacctcaggg atattgatat
     4741 taaacgataa atcggcaaag cgtcctgcag aattcagttg tatccgttgt gcccgatgca
     4801 ctcatgtctt ccctatgggt ttggaaccct atctcattaa aaatatggtg ctg
//
LOCUS       DAOOQP010000169         4838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_20967_length_4838_cov_5.780054, whole genome shotgun sequence.
ACCESSION   DAOOQP010000169 DAOOQP010000000
VERSION     DAOOQP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20967_length_4838_cov_5.780054"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1065
                     /locus_tag="PLK75_12215"
     CDS             247..1065
                     /locus_tag="PLK75_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40053.1"
                     /translation="MRFLIIVFIGIGIFNCSLTPALGQGTSSGSLKAVLIVGYQEDGT
                     LDAIEKMDKIAKLLKENGVSVYKFYDSNADWNEITKVSGECSFFIYSGHGSRLGEDGN
                     VGGLCITTMVSTADILKDLHLKSNALVVFVSVCNGAGSSAGDDDDIGITEAKKRVISY
                     AAPFFKIGAAAYYANNYDNGPYNFLKDFFSGESLKKAYLNSTTTWTDVEFDEPFPGHP
                     DKTFSIASEQGGGIATRTTYINGVKKVEEITDPKGYEITYAGKPDFTIATMKLR"
     gene            1379..2851
                     /locus_tag="PLK75_12220"
     CDS             1379..2851
                     /locus_tag="PLK75_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOY40054.1"
                     /translation="MNVTIENILLVGSLLLFISIIVGKTSYKFGVPTLLLFLAIGMLA
                     GSEGIIGIHFNDAKIAQFVGVVALNFILFSGGLDTNWASVKPILREGIALSTAGVLLT
                     ALILGGFVWLITDFSFYESMLLGSIVSSTDAAAVFSILRSKSLALKSRLRPTLELESG
                     SNDPMAYVLTIAFLTLVINEDQSILSIIPMFLQQMILGALAGYAFGMFSRFIINKSNL
                     GFEGLYPVLVIALMFITFSATDFAGGNGFLAIYICAVYLGNQDIIHKKTIIKFYDGVA
                     WLMQIVLFLTLGLLVFPSQIIPYLGIGMLISVFLIVVARPVSVFLSLAFFKMKLRRRF
                     YISWVGLRGAVPIVFATYPLLAGIEVANIIFNIVFFISVTSVLIQGTTLSVIAKWLHV
                     SLPERIKPSSELDKFINEIPKSTMKEIVISTDCGAVNRRIVDLGFPKNSIIAMIKRNN
                     RYITPNGSTVIEPNDVLLVLSDNEESIEQVNLCLFQGEKS"
     gene            3209..4690
                     /gene="hutH"
                     /locus_tag="PLK75_12225"
     CDS             3209..4690
                     /gene="hutH"
                     /locus_tag="PLK75_12225"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOY40055.1"
                     /translation="MYVHIIKPESLRLSDLERILQKPTKLELSEESIHLIQKCRTFLD
                     RKLSNSDEPVYGINTGFGALCNISVSKESLAVLQKNLVQSHACGAGTMVEPDIVKIMM
                     VLKIHALSLGKSGIQLATVQKLIELFNHEIFPVVYELGSLGASGDLAPLAHMVLPLLG
                     SGKAHYKGRIYNTENLFTELNISPIELGSKEGLALLNGTQFMSAHGVVALILAGKLSR
                     IADSVAALSLDAYNGRPDPFDELLQQVRPHAGQIETARNIRKILKGSQLIGQKKMHVQ
                     DPYSFRCIPQVHGAVKDVISHVAGIVATEINSVTDNPTIFPDDNRIMSGGNFHGEPLA
                     MAYDYMAIALSELGSISERRLYRLISGQRGLPEFLVSNPGLNSGFMIPQYTAASVVSQ
                     NKQLCTPASVDSISSSNEQEDHVSMGANAATKLLKVCNNLKILLSIEFFAAAQAIEFR
                     RPAQTSPVLEAVLNEYRKQVHFVENDCLMHDEIQKTIDFLLNI"
BASE COUNT         1436 a          961 c         1008 g         1433 t
ORIGIN      
        1 tcccaattat ggtattgaat acgcatttaa gttcagaaac aaaaacagca gtaacacaca
       61 gtacgcgatt gatgtgatag gaaatgaagt actggggaaa agagattgta taatgagtca
      121 aaaggtaatt tctgtcaaaa aactcaactc ttcaagcacc tgaataaaaa gacatactta
      181 atagcacaaa atcacagcat actaaacgcc ttgcttttca cgttaaatgt aaagaaacaa
      241 aagaaaatga gatttctaat cattgtattt atcggaattg gcatattcaa ttgcagctta
      301 actccggctt tggggcaagg cacttcatcg gggagtttaa aagcggtgtt gattgttggt
      361 tatcaggaag atggcaccct cgatgcaatt gaaaaaatgg ataagattgc gaagctgctc
      421 aaagaaaacg gtgtaagcgt atataaattc tatgatagta atgccgattg gaatgagatt
      481 accaaagtgt cgggagaatg cagctttttt atttactccg ggcacggaag ccgattggga
      541 gaagatggca atgtgggagg tctgtgcatt accacaatgg tttctaccgc cgacattctg
      601 aaagacttgc atttaaaaag caatgcgctg gttgtttttg tttcggtatg caatggagcc
      661 ggttcttcag ccggcgacga cgatgatatt ggtattactg aagcgaagaa acgggtaatc
      721 agttatgcgg cacctttctt caagattgga gctgcagcgt attatgcaaa caactatgat
      781 aacggtccgt ataatttcct gaaagatttt ttctcgggag aaagcttgaa aaaagcgtat
      841 ctgaattcca ccacaacatg gaccgatgtt gaattcgacg aaccctttcc ggggcacccc
      901 gataaaacct ttagtattgc cagtgaacag ggaggtggta tagcgaccag aacaacatac
      961 attaacgggg tcaaaaaagt ggaggaaata accgacccaa agggatacga aattacctat
     1021 gccgggaaac ccgactttac gattgccaca atgaagttgc gttgaataga ttggcatagc
     1081 tgactcaggt caatgttcaa gttacttttt aattatcatc ttcttgatta accaaatttg
     1141 aaaccccata cggctccagg tagaatttat tcaaatttta ctggttccat gatttgttat
     1201 cacagggaat ataaaagatt caaaaaaata tcctattttt aagagttgaa atacatgaaa
     1261 actcagggta aaattatatg gatataatgc aaataaatag gaattgccat aaccttgatg
     1321 caaattttaa tgtcagcagg ttcataggat tgcaccaaat tactgaaaag cttgccctat
     1381 gaatgttact attgaaaaca tacttttagt tggttctctg ttacttttta tcagcataat
     1441 tgtgggtaag acctcctata aattcggcgt gcccacatta ttattatttc tcgctatcgg
     1501 tatgcttgcg ggttctgaag gtattatcgg catacatttc aacgatgcca aaatcgccca
     1561 atttgtaggg gttgttgctt tgaattttat actattttcg ggaggacttg ataccaattg
     1621 ggcatcagtg aagcccatac ttcgtgaggg cattgcctta tcgactgcag gagttttgct
     1681 tactgcatta atactgggtg gttttgtatg gctgattact gatttctcat tttacgaatc
     1741 catgcttttg ggctcaattg tgtcatcgac cgatgcagcc gcagtgtttt cgatacttcg
     1801 gtccaaaagc ctggctttaa aatcacgctt gcgacccact cttgaattgg aaagtggaag
     1861 caacgaccca atggcatatg tactgacgat agcatttttg acattggtta ttaacgaaga
     1921 tcagagtatt ctctccatta tccccatgtt tttacagcaa atgatattgg gagctctggc
     1981 aggttatgct ttcggtatgt tcagcaggtt catcatcaat aaaagtaatc ttggtttcga
     2041 ggggctctac cctgttctgg ttatagcatt gatgtttata acattctcgg ctaccgattt
     2101 tgcgggtggt aacggttttc tggcaatata catctgtgcg gtgtatttag gcaatcagga
     2161 tatcatacat aaaaaaacca tcatcaaatt ttacgatggc gtggcatggc tgatgcaaat
     2221 tgttcttttt ctcacactgg gattactcgt atttccttcg caaataattc cctatttggg
     2281 tattggcatg cttatttctg tattcctgat agttgttgcc aggcctgtta gcgtttttct
     2341 gagtctggca ttttttaaaa tgaaactccg gaggcgattt tatatctctt gggttggttt
     2401 gcgtggagcc gttcctattg tttttgcaac ctacccactt ctggcaggga tcgaagtggc
     2461 aaatattatt ttcaacatcg tgttttttat ttcggtaaca tctgtgctga tacagggcac
     2521 tactttatca gttatagcaa aatggcttca tgtttcgctc ccagaaagaa taaagccttc
     2581 ttctgaattg gataagttta taaacgaaat tcccaaatcg accatgaaag agatcgtgat
     2641 ctccaccgac tgtggtgccg taaaccgacg aattgtagat ctggggtttc caaagaactc
     2701 aatcatagcc atgataaagc gaaataaccg gtatattacc cccaacggct ctacagtgat
     2761 tgaaccaaac gatgttttgc tggtgctttc cgataatgaa gaaagtatcg aacaggtgaa
     2821 cctttgtttg tttcagggag aaaaatcgta attaagcaat ggggggctga ccgttaacga
     2881 atacaattaa aactattcct tcaaattcat tataaggatt taattcgcca cgaaaatcag
     2941 ttatggggcg agtactgtac tatcgtcagc gctccaaact aactttcttc aactgacgca
     3001 ttctaaaaaa ttcaaaatat caggtgtgct aataaatcag aattgttttc ggaatgataa
     3061 tctaatttaa aaaaaacata tagctttgta aaaatttcgt tatgtggatt attgatttca
     3121 tcatttgcac aaaccgtaag tcgttgtttt ggcgataaat ttcgttttgg agatcaatat
     3181 tggtaatcac tattaaaaat caaaaaaaat gtacgtacat attattaaac ctgagtcact
     3241 caggcttagc gaccttgaaa gaattctgca gaaaccaaca aagcttgaat tatcggaaga
     3301 atcaattcat ctgattcaaa aatgccgcac atttctcgac cgtaaattat ccaactccga
     3361 tgaacccgtg tatggtatca ataccggatt tggggcatta tgcaatattt cggtatcgaa
     3421 agaaagcctt gctgtattgc aaaaaaacct ggttcagtcg catgcatgcg gcgcggggac
     3481 catggttgaa cccgacattg taaaaatcat gatggtactg aaaattcacg ccttgtcgct
     3541 cggaaaatca ggcattcaac tggcaacagt gcagaaactg atagaattgt ttaaccacga
     3601 aatctttcct gtggtatatg aactggggtc gcttggagct tcgggcgacc tcgctcctct
     3661 tgcacacatg gttcttcctt tgctgggttc cggaaaggca cattacaaag ggaggattta
     3721 caatacggag aatcttttca ccgaactgaa tatttcccct attgagcttg gatccaaaga
     3781 aggactggct ttgcttaacg gaacacaatt catgagtgct catggtgttg ttgcattaat
     3841 tctggcagga aagctgtcgc gaatagccga cagtgtagcc gccctctcgc tcgacgcata
     3901 caatggccga ccagaccctt tcgatgaact cctgcaacag gtacgtccgc atgccggtca
     3961 gattgaaaca gcacgcaaca tcagaaaaat tctgaaaggc tcacaactca tcgggcaaaa
     4021 gaaaatgcat gttcaggacc cctactcatt tcgatgcata ccgcaggtgc acggagctgt
     4081 aaaagatgta atttcgcatg tagcgggtat tgttgctaca gaaattaatt ccgtgaccga
     4141 taaccctact attttccctg atgacaaccg aatcatgtcg ggaggaaatt ttcatggtga
     4201 accacttgcc atggcatacg attatatggc tattgcactc tccgagctag gtagtatcag
     4261 tgaacgccgc ctgtacagac ttatttccgg acaacgtggc ttacccgaat ttctggtaag
     4321 taatcccggt ttaaacagcg gatttatgat accccaatat acagcagcct cggttgtcag
     4381 ccagaataag caactgtgca cgccagcctc cgttgattcg atttcctcat cgaatgaaca
     4441 agaggaccat gtaagtatgg gtgcaaacgc tgcgaccaaa cttctcaagg tttgcaacaa
     4501 cctcaaaatc cttctgagta tcgaattttt tgctgccgca caagccattg aatttcgccg
     4561 ccctgcccaa acctcaccgg tgctggaagc ggttcttaac gaataccgta aacaagtaca
     4621 tttcgttgaa aacgactgcc tgatgcatga tgaaatacaa aagactattg actttctgct
     4681 gaatatttaa actgttttgt tttttcggtt ttcgctatca attactaaca acatcagtta
     4741 ttatttaatt cgaaattcgt ttgatttttc atacattttt ttacactgat gtccgataaa
     4801 aacttttaaa gaagccagcg cataagtctg gcttcttc
//
LOCUS       DAOOQP010000170         4719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_21623_length_4719_cov_4.783019, whole genome shotgun sequence.
ACCESSION   DAOOQP010000170 DAOOQP010000000
VERSION     DAOOQP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21623_length_4719_cov_4.783019"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLK75_12230"
     CDS             complement(<1..218)
                     /locus_tag="PLK75_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40056.1"
                     /translation="MNFDKLTDHLKSALANPLPGEDAQRLMTPASRQLEIRYFSVEPL
                     RKAAVLICLFPVGDRTHTILIERTQDAGP"
     gene            complement(237..1625)
                     /gene="gatD"
                     /locus_tag="PLK75_12235"
     CDS             complement(237..1625)
                     /gene="gatD"
                     /locus_tag="PLK75_12235"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOY40057.1"
                     /translation="MNNNDLFQGYKGKALELLMKYNVRVWGYAEMETTRGSFKGTVLP
                     RSINDDAEHIVIKVFTGYNIGVDVNTIISMQETGYKKANYAIPEKQFPYTEGLPHVKL
                     FGTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLETEKLFAVFSENMGPKQY
                     IALAHAIQREVENGIEGIVIGHGTDTLHHTGAALTFMCQNLPVPVVLVGSQRSSDRPS
                     SDAALNLMHAMKTAGTGDIAEVLVCMFGPTSDEYGFLHKGTRVRKMHSSYRSTFRTIG
                     DTPVATVTRNGVVPIKKEYNRRRNDRKMILKPYFNDRVTMLYYYTGMNPDILDAMIDL
                     GYKGIVIVGTGLGHVNKELYPAIERAQKKGVHIYMTLQTIWGYVQMFVYDTGRDMMSK
                     GIIPLGNMLPEVAFIKLSWALGQTDDPAEVKRLMLTPVNDEITEKEPYNGYLVYQGGV
                     PEVEDFVRKVRK"
     gene            complement(1652..3640)
                     /gene="gatE"
                     /locus_tag="PLK75_12240"
     CDS             complement(1652..3640)
                     /gene="gatE"
                     /locus_tag="PLK75_12240"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOY40058.1"
                     /translation="MKKAFDPYQNYLRTREKIGYVDRKNATQADYDRIGFMSGLEVHQ
                     QLKTKKKLFCHCPAGIFHADDEYNAEVIRHMRPTLSELGEYDGTALMEFKTRKNIVYR
                     INNQTACTYEVDDTPPFLINNEALDIAFQITMLCKMNLVGEVHVIRKQYLDGSIPTGF
                     QRTAILGVEGNIQLKKKTVRLMQLSIEEDSCREISDIGHWRIYKTDRLGMPLIETVTY
                     PDFVNPDEVKEGCDIIRFINRSTGNVRTGIGASRQDVNVSCKGGTRIEIKGVAHTKWI
                     PELTHVEAFRQYALLALRDELLRRFPDPTAWKPEAVQLDYDAFQVTYPPIKEARDRGD
                     KLYAVNLPGFKGIMSHFTQPTKMFADEISDRLKVIACIEKPHMIHTEDFEPVITDADM
                     EILKMMMKSNPDKDAIIVFWSPADDVKTALETIEERCRMAFLGVPNETRKSFEDGSTI
                     FERVLPGADRMYPDTDTAPIPIAESHLNELAKGLPTDINELKLMLNTWNVPEEMHLFL
                     LSKNLVPLIKRIEKDLKVDARRVAVMMAKRLKYVEGHYTKANDFKYDRLYDLIKFACD
                     EKLECPLMQRMLPVVFEHPKMDFHSVLTTIKFKRYSHEDILSKIDFLVRKFEPIGRNK
                     TRNNTINWIMGQLRPMAEGNISLTELSRAVSEKIDNLK"
     gene            3972..>4719
                     /locus_tag="PLK75_12245"
     CDS             3972..>4719
                     /locus_tag="PLK75_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOY40059.1"
                     /translation="MISVENEIVTSAKSLRSRLHAIPELSGEEILTAEVLAAYARENG
                     ADEVITQLADRSLAVIINGKSNGKTLMLRCDIDAIPSGSSAAHLCGHDGHSAVMAGLI
                     PFLKIHPPMAGRVILLFQAAEETGQGAKALLNDTHFLSMKPDFIIGMHNLPGFEKNSI
                     VFKNNEFAMASNGVLARLLGKKCHAAEPEKGINPAAAVADFIGELQRMNESVNTEANF
                     QFATITHIRVGETTFGTAAADAEIWITLRAS"
BASE COUNT         1252 a          997 c         1063 g         1407 t
ORIGIN      
        1 ggtccggcat cctgagtgcg ttcgataagt attgtgtgtg tgcggtcgcc aacagggaaa
       61 aggcatataa gcactgctgc cttgcgaaga ggctcaacgc tgaaatatcg tatttcaagc
      121 tgcctcgaag caggtgtcat aagacgctga gcgtcttcac cgggcaaagg atttgcaagt
      181 gccgatttta gatgatctgt gagtttgtcg aaattcaaca gtgcagtaaa tacttgttat
      241 ttccgaactt tccgaacaaa atcttcgact tcaggaacgc ctccctgata aacgaggtac
      301 ccattgtaag gctctttttc agtaatttca tcgtttacag gagtgagcat cagtcgcttt
      361 acttcggcag ggtcatcggt ttggccgaga gcccatgaaa gcttaataaa agcaacctca
      421 ggaagcatgt ttcccagagg aataatacct ttcgacatca tatcgcgtcc tgtgtcgtaa
      481 acaaacatct gaacataacc ccagatcgtc tgcaaggtca tataaatgtg aacgcccttt
      541 ttctgcgctc tttcgatggc agggtaaagt tccttgttga cgtgaccaag acctgtgcca
      601 acgattacaa tacctttata tccaaggtca atcatggcat ccagaatatc cgggttcatc
      661 cctgtgtaat aatacaacat agtaaccctg tcgttgaaat agggtttcag tatcattttg
      721 cggtcgttgc gacggcggtt gtattctttt ttgatgggaa caacaccatt gcgggtaact
      781 gtggctaccg gtgtgtcgcc gattgtacgg aatgtggaac gatatgaaga gtgcattttc
      841 cgaacacggg tacctttatg cagaaagccg tattcatccg atgtcggtcc aaacatacat
      901 accagaacct cggcaatatc acctgtcccg gcagttttca tagcatgcat aaggttcagt
      961 gctgcatcgg aactgggccg atcgcttgaa cgctgcgaac caacaagaac aacaggaact
     1021 ggtaggttct gacacatgaa agtgagtgca gcaccggtgt gatgcagtgt gtcggtacca
     1081 tgacctataa caatgccttc gatgccgttt tccacttctc tttgtatggc atgggctaag
     1141 gcaatgtatt gcttgggtcc catattttcg ctgaaaacag caaagagttt ttcggtttcc
     1201 agattacaga tatcagccaa ctccggcacc gcaccgtata gttctccggg agaaaaggcc
     1261 ggtataaccg ctcctgtacg gtaatcaagt cgtgaagcaa tagttccccc tgtcccaaaa
     1321 agttttacat ggggcaagcc ctctgtgtag gggaattgct tctcaggtat tgcataattg
     1381 gcttttttat agccggtttc ctgcatcgaa ataatggtgt tcacatcgac gccgatattg
     1441 taacctgtaa agactttgat cactatgtgc tcggcatcat cgttgataga acgggggaga
     1501 acagtgccct taaatgagcc gcgggtggtt tccatttcgg cataacccca tacccgaaca
     1561 ttgtatttca ttaacaattc aagtgccttg cctttgtatc cctgaaacaa atcgttattg
     1621 ttcatctgtt tacgaaatta ttgtgtaaat actacttcaa gttgtcgatt ttctcactca
     1681 cggcgcgact tagctcggta agcgatatgt tgccttcagc cattggtcgc aattgcccca
     1741 taatccagtt aatggtgttg tttctggttt tatttcttcc gatgggttca aatttgcgaa
     1801 ccagaaaatc aattttactg agaatatctt cgtgcgaata ccttttaaat ttaatggttg
     1861 tgagtacaga atgaaaatcc attttcggat gctcgaaaac taccggaagc atacgttgca
     1921 ttaacgggca ttcaagtttt tcgtcgcatg caaacttaat aaggtcgtac aaacgatcgt
     1981 atttgaaatc gttggcttta gtgtaatgac cttcaacata tttcaggcgt tttgccatca
     2041 taaccgcaac cctgcgggca tccactttca aatctttctc aatgcgttta atcagaggaa
     2101 caagattttt gctcaaaagg aataagtgca tttcttcagg tacattccat gtgttgagca
     2161 tcaacttcag ttcgttgatg tcggtaggca aacctttggc gagctcgttc aggtgcgatt
     2221 cagcaattgg aatgggagca gtatccgtat caggatacat tctgtcggca cccggcaata
     2281 ccctttcgaa aatagtagaa ccgtcttcaa acgatttgcg ggtttcgttg ggtacaccta
     2341 agaatgccat gcgacaacgc tcttcgatgg tttcgagagc tgttttaaca tcatcggcag
     2401 ggctccagaa tactataatc gcatctttat cggggttcga tttcatcatc atcttgagaa
     2461 tttccatatc tgcatcggta attacaggtt cgaaatcctc ggtatgtatc atgtgcggtt
     2521 tctcgatgca agcaatcacc ttgaggcggt cgctgatttc atcggcaaac atctttgtgg
     2581 gttgggtaaa atgcgacata attcctttga atcccggcaa attaacagca tacaatttat
     2641 cgccgcggtc acgtgcttct ttaataggag ggtatgttac ttggaaggca tcgtaatcga
     2701 gttgcactgc ttcaggtttc catgcagtcg gatcggggaa gcgtcgcagt aattcatcac
     2761 ggagtgccaa cagtgcatat tgacggaagg cttctacatg ggtaagctcg ggaatccatt
     2821 ttgtgtgggc aacgcctttg atttcgatac gtgttcctcc tttacaactt acatttacat
     2881 cctgcctcga tgcaccaatt cctgtgcgga catttcctgt gcttctgttg atgaatcgga
     2941 tgatgtcgca accttcttta acttcgtcag gatttacaaa gtcgggatag gttacggttt
     3001 caatcagggg catcccaagt cggtcggtct tatagatgcg ccagtgcccg atgtcggata
     3061 tctcacggca ggagtcttct tctatgctga gctgcatgag cctaacggtc tttttcttca
     3121 gttgaatatt gccttctaca cccagaatgg ctgtacgctg aaaaccggtt ggaatactgc
     3181 catcgagata ttgctttctg attacatgca cttcgcctac cagattcatc ttgcataaca
     3241 tggttatctg aaatgcaata tcaagggctt cgttgttgat aaggaagggt ggtgtgtcgt
     3301 caacttcgta ggtacaggca gtttggttgt tgatgcgata cacaatgttt ttacgggttt
     3361 tgaactccat aagtgctgtt ccatcatatt caccaagttc gctcaatgtg gggcgcatat
     3421 gccgtattac ttcggcattg tattcatcat cggcatgaaa aatacctgcc ggacaatgac
     3481 aaaatagctt tttcttagtt tttaactgct gatgaacttc aagccctgac ataaatccaa
     3541 tgcggtcata gtcggcctgg gtagcgtttt tgcggtcaac ataaccaatc ttttcacggg
     3601 ttctgaggta attctgataa ggatcgaatg cttttttcat gaatatccgt ttttacggtt
     3661 taaatttcga cctcaaaagt aaatttgttt tagcacataa acaaggtatt tttttaacat
     3721 tttgcggtaa aaagttatta attttaaccg catagctaca gaattttgaa aattatttat
     3781 aaaataaaat tattagctgt tttaggttgc aataattaat aatatatcga aaaattaatg
     3841 tgttttgtgg gttaaataca tgcagtcctt tttttttcag gttaatgaat tttggtagct
     3901 aatcatgatt tctttgagga aacaacaatt ttttactgct ttttattaat tttactaatc
     3961 aaattgaagg tatgatcagt gttgaaaacg aaatagttac aagcgcaaaa tcattgcgca
     4021 gccgtcttca tgcaataccg gaactttcgg gtgaggagat actgacggca gaggtgctgg
     4081 cagcctatgc ccgtgaaaat ggagccgacg aagtaataac ccaactggct gaccggagtc
     4141 ttgcagtaat aataaacgga aaatctaatg gcaaaaccct gatgcttcgc tgcgatatcg
     4201 acgctattcc ttcaggtagc agtgctgcac atctctgtgg tcacgacggg catagtgcag
     4261 taatggctgg tttgattcct ttcctgaaaa tacaccctcc aatggcaggc cgtgtaattt
     4321 tattgtttca ggcagcggag gaaaccggac agggagccaa agcattgcta aatgatacgc
     4381 actttttatc gatgaaaccc gactttatca tcgggatgca caatctgcca ggttttgaaa
     4441 agaatagcat tgtgtttaag aataatgaat ttgcgatggc ttcgaatggg gtactggctc
     4501 gtttacttgg aaaaaagtgc catgctgcag aacccgaaaa aggcattaat cctgcagcgg
     4561 cagtagcgga ttttattggc gagttgcagc gtatgaatga gtccgtcaat acggaggcaa
     4621 attttcagtt tgcaaccatc acgcatatca gggtaggaga aactactttt ggaactgctg
     4681 cagcagatgc cgaaatatgg ataactctca gggcttcca
//
LOCUS       DAOOQP010000171         4712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_21662_length_4712_cov_5.184239, whole genome shotgun sequence.
ACCESSION   DAOOQP010000171 DAOOQP010000000
VERSION     DAOOQP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21662_length_4712_cov_5.184239"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLK75_12250"
     CDS             <1..277
                     /locus_tag="PLK75_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40060.1"
                     /translation="VTNIPTGINGQTSSDLIAHIYPNPATDKLNIAFTGAGSKSISMT
                     NLLGQTVYRTETSEKQLSIDVSRYDKGMYFIVVYNNNQKSQVKVVIE"
     gene            complement(523..1884)
                     /gene="hisS"
                     /locus_tag="PLK75_12255"
     CDS             complement(523..1884)
                     /gene="hisS"
                     /locus_tag="PLK75_12255"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOY40061.1"
                     /translation="MSFKPSIPKGTRDFGPLAATQRNYIFETIRKVFELRGFRPIETP
                     AMENLSTLTGKYGDEGDKLLFRILNSGNFLAETDKTVVLQGNSQAVASQISEKGLRYD
                     LTVPFARFVVQHQNEIVFPFKRYQIQPVWRADRPQKGRYREFYQCDADIIGSSSLVNE
                     FEMILLIDNVFEKLNIKTHIHINNRKVLFGIAEAFGFGDSFQDFTVVIDKLDKIGSDN
                     VKAELTATGIDSTKADAILELLAFSGCNREKLAKLETIFTTSETGRKGLEEMQELFEY
                     ISNYNGNAKVIFDISLARGLNYYTGAIFEVKAIGHAIGSICGGGRYDNLTGIFGLPNV
                     SGVGISFGADRIFDIMEQSNLFPEHIESSAKIMFVNFGKTEQKAALEIIATLHKAGIS
                     AFLYPDTAKMKKQLQYADQLRIEYIAIIGEDELKNNNLSLKNMHTGEQTACTAKELIG
                     LLK"
     gene            complement(1895..2992)
                     /gene="ychF"
                     /locus_tag="PLK75_12260"
     CDS             complement(1895..2992)
                     /gene="ychF"
                     /locus_tag="PLK75_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOY40062.1"
                     /translation="MGLHCGIIGIANSGKTTLFNCMSSKKAEATSFAFATNKSNIGVI
                     SVPDERLYELEKFQKTEKIVHATVEIVDMPGLTKGSSKGEGVGNQFLADIRNADAIIH
                     VLRCFDDELLPHVDGSVNPVRDMETIDIELQIKDLESIEKKISRLEKVAKIGDKDAKH
                     HVEVLNIYKNHLENLGNARTAPVNPDDRSVVEDLSLLTDKPVLFVCNVDEASALNGNR
                     YTEAVKEALAGTDSKVLIIAAKLEADIAEFETKEERMAFLEDYGLNEPGVYKLIRAAY
                     DMLNLQSFFTVGPKEIKAWTIKKGMTAPEASGVIHSDLQRGFIRAEVMRYEDFVHYGS
                     EQSVKDAGKFKVEGKTYVVQDGDIMHIRFNV"
     gene            3169..4050
                     /locus_tag="PLK75_12265"
     CDS             3169..4050
                     /locus_tag="PLK75_12265"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HOY40063.1"
                     /translation="MKRIITLAILFAVLLSVFWACTTGGNNNQTRPVMYAMVNSVNWR
                     CADPHARISENSIVVYGTSANGQTIQLRILAGEKGEYALNMQTGHEGKFIPNMSDAVV
                     AYSTNSSTQGTGMAIINSINEESKTVSGSFYFKGYRATDGSFKSITDGTFSNVPYKFI
                     NTVDTSVFDNVMYATVSDESWVPNQITAVKNDTAIIITGTLSSSWESLTIILPLNTGA
                     GVHYITAEGPVIARFQQAFYTYVGTAGSCTISQHDTERQIIRATFFFNFVDNNNVTHS
                     ITAGNFEVMYIDESAEE"
     gene            complement(4187..>4712)
                     /locus_tag="PLK75_12270"
     CDS             complement(4187..>4712)
                     /locus_tag="PLK75_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOY40064.1"
                     /translation="EMAGMVITMVSVLSERRMNRLNNPALSVGLPAFLTKGAGMFSGL
                     MLSQYTADMQIVEQKLLSTPAAIQSIPAAADQEDFVSMGMNTALKNNQILDNAYGVLG
                     IEFMAAAQALDFRKEEIGKFGVGTTKAWEVVRRHVDFLDIDRPLHTDHNAMKKLVKSC
                     EILEEVEKVTGSLG"
BASE COUNT         1296 a         1064 c          949 g         1403 t
ORIGIN      
        1 cgttaccaat atccccacgg gtatcaatgg tcagacaagc agcgacctga ttgcgcatat
       61 ctatcccaac cctgccaccg ataaacttaa cattgccttc accggtgcag gcagcaaaag
      121 catcagtatg accaacctgc tgggacaaac cgtctatcgc accgaaacct ccgaaaagca
      181 gctaagcatc gacgtcagcc gctacgataa aggcatgtac ttcatcgtcg tatacaacaa
      241 taaccagaaa tcgcaggtta aggtggtaat tgagtagtac ttgctgtaac cgcccgtcaa
      301 atacagattg cttcatgttc gtacctcaca ttcgcaatga cgggttctcc cacaacgtca
      361 ttgcgagcgc agtgaagcac ttgtgctgag cgaagtcgaa gcaatctccg tacccgtagt
      421 cgctactatc attgcccgcc aaatgcctgt gttggcctgt cgaaattcag attgcttcat
      481 gttcgtacct cacattcgta tgacgggctt tccctcacgt atttatttca gaagaccaat
      541 cagttctttg gcagtgcagg cggtttgttc tccggtatgc atgtttttca ggctcaggtt
      601 gttgttcttc agttcatctt caccgattat ggcaatatac tcaatcctga gttggtcggc
      661 atattgcaat tgctttttca tctttgcagt atcaggatac agaaatgctg aaatgcctgc
      721 cttgtgcagc gttgcaatga tttctaaagc agccttctgt tcagttttcc caaagttcac
      781 aaacatgatt ttagcagaac tttcgatatg ctccggaaaa agattgcttt gctccataat
      841 atcaaatatt ctgtcggcac caaacgaaat acctactccc gaaacattgg gcagcccgaa
      901 aattccggta aggttgtcgt atcttccgcc gccacaaata cttccaatcg catgaccgat
      961 agctttaact tcaaatatag ctccggtata atagtttaac ccacgggcaa gcgatatatc
     1021 gaaaatcact ttggcatttc cgttatagtt ggatatgtac tcaaacagct cctgcatttc
     1081 ttcgagccct tttcttccgg tttctgaggt ggtaaatatt gtttcgagtt ttgcaagctt
     1141 ctcacgattg caccctgaaa atgccagcag ttccaatatt gcatcagctt ttgtgctatc
     1201 aataccggtt gcagtaagct cagctttaac attgtcgctg ccgattttat cgagtttgtc
     1261 gatgacaact gtgaaatcct gaaaactatc accaaacccg aaagcttcag caatgccgaa
     1321 aagcactttt ctgttattga tatgaatatg agttttgatg ttcagttttt cgaaaacatt
     1381 gtcaataagc agaatcatct cgaattcatt tacaagtgat gaggatccga taatgtcggc
     1441 atcgcattgg tagaattcac ggtagcgccc tttctgaggt cgatcggctc tccaaacggg
     1501 ttgtatctga tagcgcttga acgggaaaac aatctcattc tggtgttgta ccacaaagcg
     1561 ggcaaaaggc actgtaaggt catagcgcaa acccttttct gatatctgtg atgctacagc
     1621 ttggctgttt ccttggagga ctacagtttt atcggtttca gcaagaaaat taccggaatt
     1681 gagtatcctg aaaagcaact tatcgccttc gtcgccatat tttccggtaa gtgtggaaag
     1741 attctccatt gcaggagttt caatcgggcg gaacccacgc agttcgaaga ctttacgaat
     1801 agtttcgaaa atatagtttc gctgcgttgc cgcaagcggt ccaaaatctc ttgttccttt
     1861 aggaatggaa ggtttaaaac tcatacagct tgtattaaac gttaaatcgg atatgcatta
     1921 tatcgccgtc ctgcacaaca taggtttttc cttccacctt gaactttccg gcatctttca
     1981 ctgactgctc cgaaccgtaa tgaacgaaat cctcgtaacg cattacttca gccctgataa
     2041 agccccgttg taggtcagaa tgtatcacgc ctgaagcctc gggagcagtc ataccctttt
     2101 taattgtcca tgctttaatc tcttttggac ccacggtaaa aaacgactga agattcagca
     2161 tatcgtaggc ggccctgata agtttgtaca ccccgggttc gttcagtccg taatcttcga
     2221 gaaacgccat tcgttcttct ttagtttcaa actcggcaat atcggcttca agctttgctg
     2281 caatgattag aaccttagaa tcggttccag caagtgcttc tttaacagct tcggtatatc
     2341 ggtttccatt taaggcagat gcttcgtcaa cattgcaaac aaacaataca ggtttgtcag
     2401 taagtaacga aagatcttca acaacactgc ggtcatcagg atttaccggt gcagtgcgcg
     2461 cattcccaag gttttcaagg tgatttttat aaatattcag cacttctaca tggtgttttg
     2521 catctttatc accgattttc gcaacttttt cgagacggct gatttttttt tcgattgatt
     2581 ccagatcttt tatctgtagc tcaatatcga tggtttccat gtcgcgcaca gggtttaccg
     2641 aaccatcaac atgtggtagc aattcatcgt cgaagcaacg aagcacatga ataattgcat
     2701 cagcattacg aatgtcagca agaaactgat tgccaacgcc ttcgccctta ctggagcctt
     2761 ttgtcaaccc gggcatatca acaatttcga cagtagcgtg tacgattttc tcggttttct
     2821 gaaatttttc gagttcatat agcctttcgt caggaactga aattactccg atatttgact
     2881 tgtttgtggc aaaggcaaag ctcgttgcct cagctttttt actcgacata cagttgaata
     2941 gggtagtttt cccgctattc gcaattccaa ttattccgca atgtaatccc atacctgaag
     3001 caaacctaaa attaataaat tcgtctatgt gttgatgcaa aaataaccta ttcgcccgaa
     3061 tgcacaaaaa atataatttt catacacaat caaccgttca taaaattgat atgcatcacc
     3121 ttttgaagtg tcttaatatg tattattttt gttagcaatt tagaaaacat gaaaagaata
     3181 ataactttag ctatattatt tgcggttttg ctaagcgtat tttgggcatg cactaccggt
     3241 ggaaataaca atcagacccg tcccgtaatg tatgccatgg taaattcggt aaactggcgt
     3301 tgtgccgacc ctcatgcacg tatatcggag aacagtattg tggtatatgg cacttcagcc
     3361 aatggtcaga ccattcagct cagaatactt gccggagaaa aaggagaata tgccctgaat
     3421 atgcagaccg gtcacgaagg aaaatttatt cccaacatga gcgatgcggt agtcgcgtat
     3481 tctaccaatt ccagcacaca aggtacaggt atggcaatca ttaattcgat aaacgaagaa
     3541 tcgaaaacag taagtggctc attttatttt aaaggatatc gtgcaacgga cggctcattt
     3601 aagtcaatca ccgacggcac tttttcgaat gttccttata agttcattaa tactgtcgac
     3661 acttctgttt tcgataatgt aatgtatgca acggtttcgg atgaatcgtg ggtaccaaat
     3721 cagatcactg ctgttaaaaa cgatacagca ataattatta ccgggacatt atcgtcaagc
     3781 tgggaaagtt taacaataat tttgccattg aatactggcg caggggttca ttacattacg
     3841 gctgaaggtc ctgtaattgc tcgatttcag caggcatttt atacttatgt tggtacagcc
     3901 ggttcctgca ccattagtca gcacgataca gaacgtcaaa ttataagggc tacatttttc
     3961 tttaattttg ttgataacaa taatgttact catagcatta ctgcagggaa ttttgaagtg
     4021 atgtatattg acgaaagcgc tgaagaatag taagtatctt aaataagcga caggcagcct
     4081 gatacgctgc ctgttttttt atgtttgtat gaaatgttgc aagctttgat aattcagcgt
     4141 ctcaactccg ctcgatgacc agctcgacta ccgaatataa tgctgattaa ccgagtgacc
     4201 cggtaacttt ctctacttct tcgaggattt cacatgattt aaccagcttt ttcattgcat
     4261 tatggtcggt atgtagcgga cggtcgatat caagaaaatc gacatgacgg cgaaccactt
     4321 cccatgcttt tgtggtgcca acaccaaact tgccgatttc ttccttacgg aaatcgagtg
     4381 cctgagcggc agccatgaat tctattccca gtacaccata ggcattgtcg aggatttgat
     4441 tgttttttaa ggctgtattc attcccatcg aaacgaaatc ctcctgatcg gcagctgcag
     4501 gtatgctttg aatagcagca ggtgtgctca gcagtttctg ttccacaatc tgcatatcgg
     4561 cagtatattg gctcagcatt agtcctgaaa acataccggc acccttggta agaaatgcag
     4621 gcaatcctac gctcagggca gggttattca ggcgattcat acggcgttca ctgagaactg
     4681 ataccatggt tatcaccata ccagccattt cc
//
LOCUS       DAOOQP010000172         4712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_21666_length_4712_cov_3.781619, whole genome shotgun sequence.
ACCESSION   DAOOQP010000172 DAOOQP010000000
VERSION     DAOOQP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21666_length_4712_cov_3.781619"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2826)
                     /locus_tag="PLK75_12275"
     CDS             complement(<1..2826)
                     /locus_tag="PLK75_12275"
                     /inference="COORDINATES: protein motif:HMM:NF026223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-repeat neuraminidase N-terminal
                     domain-containing protein"
                     /protein_id="HOY40065.1"
                     /translation="MKYLLLFVTLSFFTISNSFSQQWLKNLPQNKSKNELTFFDYQNA
                     FYSYWDQYHVKDGYYYDNGEKKKARGWKQFKRWEYLMEKQVNPGTGEFPTTTAEEQVR
                     LYYKSHPQAKSSNGNWTFMGPNTSDGGYAGIGRLNCIAYHPTDNNTYWVGAPSGGLWV
                     TTNNGTSWTCLTNDNAVLGVSDIVIPSNFATSNTIYIATGDRDAWDNNSVGVLKSTDG
                     GATWNTTGLSFSANSYRMVNRLMLDPANDNIIIAATNNGVYKTTNGGTNWSLLSSNNF
                     IDMEYKPGSFNTLYGSTSSGDIFVSTNAGSSWTQALNLSEGERVELAVSANQPAWVYA
                     VVAASDNGLYGIYKSTNSGSSFAQVFSGSTKNLLGWESDGSDAGGQGWYDLSIAASPT
                     NASIVLVGGVNTWKSTNGGTSWAIANHWWGDGVPAAHADKHMLSYRSNGDLFECNDGG
                     VYISTNNGTSWTDKTDGIVISQMYKLSVAKTNQNEVITGLQDNGTKLIATGTWYDVKG
                     GDGMECLIDYTDLNIQYGTYVNGQIDRTTNHWGSATDISPNGEPNGAWVTPYIIHPTA
                     PQTIFVGYENVYKSTNRGDSWSVISTMNTSDKIRSMAIAPSNPSVLYVADPSTIWKTT
                     NGGTSWTTVTGTLPVSSASITYIAIKNDDPNTVWVTMSGYTNPGVYQTTNGGSSWTNI
                     SGGLPSIPVNTVVQNKLNTTAVELYCGTDYGVYYKSGSSNWTIFSNGLPNVIVNELEI
                     WYGATSANSKLRAATYGRGLWESNLFSNSAMTYVSCTTTQDVTANVAQNSVNQQVIGI
                     EIITNGDLSPLSATSFTFNTNGTTNPAGDVSNARLFYTGSVAAMSTSTQFGEAIVSPA
                     GSFTITGTQLLGSGINYFWLAYDITADAAIGNYIDAECTSLTITDVKTPTVTSPTGNR
                     RISVVYCDAGSPDVSYEYISGATIGNIN"
     gene            complement(2950..3393)
                     /locus_tag="PLK75_12280"
     CDS             complement(2950..3393)
                     /locus_tag="PLK75_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOY40066.1"
                     /translation="MDEKIENFILNLPDERKHAFMQLRDTIKHNIPKGFEEQLSNNMV
                     EYVVPHSIFPGGYHANPKAPLPFISIASQKNYISLYHMGLYADNELKQWFLSEYPKFI
                     NGKPDMGKGCIRFKKSGQIPYDLLAQLVSKVSVNQWIEKYLSIIG"
     gene            3587..>4712
                     /locus_tag="PLK75_12285"
     CDS             3587..>4712
                     /locus_tag="PLK75_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOY40067.1"
                     /translation="MNAIVFISLLMLSFGVFAIPKRFKYYYALLIMLISVAITSWWAL
                     QIFRGSGSFSEIGVSLPFLHSKFLLVIDRLSAFFILIINLTVTIGFLYAYGYLKPYFS
                     EKNSLRFSIHYFSYLWLYFSMLLVSTTRDGFSFLVVWEIMALSSFLLVIFDAEERSIM
                     KTGINYLIQMHIGMAFILTALLIVEKSTGTMSFDALPEYFSSHSNILVFCLFFIGFGI
                     KAGFVPIHTWLPQAHPAAPSHVSGVMSGVMIKMGIYGIVRVLFSVQQDLLVIGVIILI
                     ISVISGLFGVMMAIIQHDLKRLLAYHSIENIGIIGIGIGLGVIGMANHNTALIMLGFG
                     GGLLHVLNHSLFKALLFYNAGSVYHATHTRNIEKMGGLMKK"
BASE COUNT         1222 a          966 c         1034 g         1490 t
ORIGIN      
        1 attgatattc ccgattgttg caccggagat atattcatag cttacatcgg gcgaccctgc
       61 gtcgcaatac actaccgata ttcttcggtt gcctgtggga ctggtgacag taggcgtttt
      121 tacatctgtt atggttagcg aagtacattc tgcatcgata taattgccta tggcggcatc
      181 ggcagttata tcataagcaa gccagaagta gttaattccg gatcccagca actgagttcc
      241 cgtgatagta aaactgcctg caggtgaaac tattgcttct ccgaattgcg ttgatgtact
      301 cattgcagca accgaacccg tataaaacag acgtgcattc gaaacatcac ctgccggatt
      361 ggttgtgcca ttggtgttga acgtgaatga tgttgccgat aaaggcgata aatcgccatt
      421 agtaataatt tcaatgccga taacttgttg attaacacta ttttgagcaa catttgcggt
      481 tacgtcctgt gtggtggtac acgaaacgta ggtcatggcg gagttcgaaa aaagattgct
      541 ttcccaaaga ccccggccat acgtggcagc ccgtagttta ctattggctg aggttgctcc
      601 gtaccagatt tctagttcat ttacaattac attgggtaaa ccgttactga aaattgtcca
      661 attgctcgaa ccacttttat agtaaacgcc ataatcggta ccgcagtata actccactgc
      721 agtggtattg agtttgtttt gaaccacagt gtttaccgga atcgaaggta atcctcctga
      781 aatattggtc caggagctcc ctccgttagt ggtctgatac actccggggt tagtatatcc
      841 gctcatggta acccacacgg tatttggatc atcgttctta atggcaatgt atgtaatgct
      901 ggccgatgat acgggcagtg ttccagttac agtagtccat gaagtgcctc cattggtggt
      961 tttccatatt gttgaggggt cggcaacata taaaactgaa gggtttgatg gcgcaattgc
     1021 catggaacgg attttatcgg aggtgttcat tgtggaaata accgaccagc tgtcgccacg
     1081 atttgtcgac ttatatacat tttcgtaacc gacaaaaatt gtttgtggtg cagtagggtg
     1141 tatgatatat ggtgttaccc atgcaccatt cggttctccg ttcggagaaa tgtcagtagc
     1201 agaaccccag tggttagtag ttcggtcaat ttgcccgttt acataggtac catattgaat
     1261 attcaggtcg gtataatcta taaggcattc cattccatca ccgcctttta cgtcgtacca
     1321 cgttccggtt gcaataagtt tggtgccatt atcctgaagc cctgtgatca cttcgttctg
     1381 gttggttttg gcaacgctta acttgtacat ttgactgata acaatgccat cggttttatc
     1441 agtccaggaa gtgccgttat tggttgagat ataaactccg ccgtcgttac actcaaaaag
     1501 gtctccgttg ctgcgatagc tgagcatatg tttgtcggcg tgtgctgctg gcacaccatc
     1561 accccaccag tgatttgcaa tggcccacga cgttcctccg tttgtgcttt tccaggtatt
     1621 tacaccgccc acaagtacaa ttgatgcatt tgttggcgac gctgcaatcg agaggtcgta
     1681 ccagccctga ccacccgcat cgctgccgtc cgattcccag cccaaaaggt tttttgtgct
     1741 gccactgaaa acctgagcaa agcttgatcc gctgttggtc gatttataaa taccatacag
     1801 tccgttgtca gacgcggcaa caaccgcata tacccaggca ggttgatttg ccgacaccgc
     1861 taattcaaca cgctctcctt cagataaatt cagagcttga gtccaactac tgcctgcgtt
     1921 tgtgctcacg aaaatatcgc ctgagcttgt tgatccatag agtgtgttga agcttccggg
     1981 cttatactcc atatcaataa aattgttcga cgacagcaac gaccagttgg ttcctccatt
     2041 ggtggttttg tacacaccgt tgttggtggc tgcaataata atgttgtcgt tagccggatc
     2101 aagcatcaac cggttcacca tccggtagct gtttgcactg aacgacaatc ctgtggtatt
     2161 ccatgtagca ccaccgtcgg ttgatttcag cacgccaacg ctgttgttgt cccatgcatc
     2221 gcggtcgccg gtggcaatgt aaattgtatt ggaagtggca aaattcgatg gaattacaat
     2281 gtcgcttaca ccaagtactg cgttgtcgtt tgtaaggcat gtccacgacg tgccgttgtt
     2341 ggtggtaacc cataaaccac ctgatggtgc accaacccag taggtattat tatcggtcgg
     2401 gtggtaagca atgcaattca acctgccaat acctgcatac ccaccatcgg aagtgttcgg
     2461 gcccatgaaa gtccagtttc cgttgctcga ttttgcttgc ggatgacttt tgtaatataa
     2521 acgaacttgt tcttcagcag tcgttgtggg gaattcacct gttcccgggt ttacctgctt
     2581 ttccatgagg tattcccaac gcttaaactg cttccagcct ctggctttct tcttttctcc
     2641 attatcgtaa taatatccgt ccttcacgtg atactgatcc caataagaat aaaaagcgtt
     2701 ctggtaatcg aaaaaggtca gctcgttttt tgatttgttt tgtggcagat tttttaacca
     2761 ttgctgcgaa aatgagttcg atatcgtaaa aaacgaaagc gtaacaaata ataaaaggta
     2821 tttcatgaaa gccgtattaa gttaataatt cctcactcac caaaaatagc aaattttgca
     2881 ttatcgttga tagtatcgaa atgcgatttt aacatattac cataaaaata tgaacaatca
     2941 atgaaaaggt cagccaatta tggataaata cttctcaatc cactgattta ctgaaacctt
     3001 cgatactaat tgagccagca aatcataggg aatctgtccg gattttttaa accggataca
     3061 tcctttcccc atatctggtt taccgttaat aaatttggga tattctgaca aaaaccattg
     3121 cttcagctca ttgtctgcat atagacccat atgatagagg ctgatataat tcttttgcga
     3181 tgcaatgctg ataaaaggca gtggggcctt tgggttggca tggtatccgc ctgggaagat
     3241 actgtgaggt accacatatt caaccatatt gttgctcaac tgctcctcaa atccttttgg
     3301 aatattgtgt ttgattgtat cacggagttg cataaaggcg tgcttccgtt cgtctggtaa
     3361 attcaaaatg aagttttcaa ttttttcatc catcgtggta tgcttgttaa aagcgtaaaa
     3421 gtacaagaaa aattttgagt agcgaaaaat gaacctttga ttgattaaac gcaatcagag
     3481 agatgtgtat cccgttagta aaaattacat tctcaatttc atgttaacga acagtttgac
     3541 taaataaaaa aaatgtaatt ttggggcttt tcagaagtaa tacataatga atgcaattgt
     3601 tttcattagt ctgttgatgc tttcttttgg tgttttcgca attccgaagc gctttaagta
     3661 ttactatgct ttgttgataa tgctgataag tgtagccata acttcgtggt gggcacttca
     3721 aatatttagg ggatccggaa gcttctcgga gatcggtgtt agtctgcctt ttttacacag
     3781 caagtttctc ctggttatcg acaggctgtc ggcatttttt attttaatca taaaccttac
     3841 ggttaccatt ggctttttat atgcttatgg atatttgaaa ccatattttt ccgagaaaaa
     3901 ctccctgcgg ttttctattc attatttctc ttatctgtgg ttgtattttt cgatgctgct
     3961 cgtaagcact acaagagatg gtttttcgtt tctggtggtt tgggagatta tggcactatc
     4021 gtcgttttta ttggtgattt tcgatgcgga ggaacgcagt ataatgaaaa caggtatcaa
     4081 ttacctgatt caaatgcata ttggcatggc ttttatcctt acagccctgc tgattgttga
     4141 aaaaagcacc ggtacgatga gtttcgatgc attgccggaa tatttttcga gccattcaaa
     4201 catcctagta ttttgcctgt tttttattgg ttttggaatt aaagcggggt ttgttcctat
     4261 tcacacgtgg ttgccccagg cgcatccagc agctccatcg catgtgtcgg gtgtaatgtc
     4321 gggagtgatg attaaaatgg ggatttatgg tattgtacgt gttctttttt cggtacaaca
     4381 ggatttgctg gttattgggg tcataattct gattatatcc gttatttcgg gtttgtttgg
     4441 agtgatgatg gcaataattc agcatgattt aaagcgattg ttggcttatc acagcatcga
     4501 aaacattggt attattggaa ttggtatagg tttaggagta attggtatgg caaatcacaa
     4561 tacagcccta attatgcttg gatttggcgg aggcttgcta catgtgctga atcattcact
     4621 ctttaaggca ctgttgtttt ataatgcagg atcggtatat catgcaaccc acacacgcaa
     4681 tatcgaaaaa atgggagggt tgatgaaaaa aa
//
LOCUS       DAOOQP010000173         4692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_21785_length_4692_cov_4.768816, whole genome shotgun sequence.
ACCESSION   DAOOQP010000173 DAOOQP010000000
VERSION     DAOOQP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21785_length_4692_cov_4.768816"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="PLK75_12290"
     CDS             <1..1607
                     /locus_tag="PLK75_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40068.1"
                     /translation="VKAEVFATQDYTITVTVNAPPPTGLASGDYVWTGAISTDWNNSA
                     NWVAFNGSNFSIPGSVPATSNDVLFTTYGTSCGITSPVISSASGNCKDITIESGHTVV
                     MSNNKILNVQGNWTNDGTFTAGNGTVVFNGSAQQTILAGGSAFNNVTFSNTTSGTTDI
                     NIGEPMIINGSATFTNGVVYYTGTGSLTFGSSATSNSGGINSYVDGVVSKTGTSAFTF
                     AVGAGPVWAPIGIAAPSVNSIITADYNFTEGPLNWAAAYMCDESQMKYTSSVEHWDLT
                     TTSATPAVTLYWTDATRSGITDLTDLAVAHFNGTCWEYKGGTTSGDLSWGSITSNINF
                     SSYSPVSFGSKRGVNKLPVELTQFGARCGDDGILITWQTASETNNDYFTLEKSENAVD
                     FIQIAEIQGAGNSVDLIEYHFLDERYVSGTFYYRLKQTDFDGSSKYSNIVIANCYANG
                     SANPVVIAYPNPFNDELSVTAWNLPEGEITLTLTDLFGKDYYRKELKTDKGEFSITLF
                     LKDIAPAVYLLKITKGEFTRTIKVVK"
     gene            1692..3359
                     /locus_tag="PLK75_12295"
     CDS             1692..3359
                     /locus_tag="PLK75_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOY40069.1"
                     /translation="MKELWNRLYGDASDCSYNEMIGLLWLYSEYNRNNPYKKPKYWYR
                     EAVVYALYVDLFAGDFKTLISKLPYLQKLGVNCLWLLPVLDSPMKDAGFDVRNYNAIR
                     GDLLTAPENQNEFDDFLDAAHSSGINVIFDIAINHTSNTHPWFQDALSSTQSRYHDYY
                     IWNKDTELYKECRLIFEGMCPCNWEKAGEEYYFHRFFEFQPDLNYKNPRVFIEMVKIF
                     LFWLKKGVDGFRLDAIPYLWKTEGTDCENLPETHLIIKLFRAIIDMVAPETLLLAEAC
                     QPPKEVVKYMGDGDECHAGYHFPLMPQIFKAIAMQSSEPVINVLKPENTPALPESCQW
                     FLFLRLHDELSLEKVYVNEDDREFLHKFYCKKNEWDFRLGQGISARLSELMDHDPNKV
                     LLLYSIMFSLQGTPLIYYGDEFGKSNDLAYYLKKKTETGYADSRNMVRGYVEWEKTLT
                     EIAREGSIENVLFHGISNMLSVRQKIGIFGTASIEMLNFECKILSYWRTIGNNKILII
                     NNISGNDVKVELPLRVNSKYDLLGNTLAISKKTLLMKPHSYHWIAVE"
     gene            3431..>4692
                     /locus_tag="PLK75_12300"
     CDS             3431..>4692
                     /locus_tag="PLK75_12300"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOY40070.1"
                     /translation="MRISFILAILLLSFLQTVQSQTLSSDYHDPDLLLLNQEIHYSTF
                     ALVQGESTELGVPECSRDYTNRNYYARFLQSYQSAVQVIARFDNQTRFGMALYLVDGE
                     QYHELRCDIFDSSEASLIMTTAENPEGLDVIVRLWVLDDVIPQQVGLCALLVSDPPGE
                     PKLMTVNTTTYTVSQLVTDVLVTGCLQASNVTFQGGATSIGYFSNATPGLDFAEGIVM
                     STGSVLDAPGPNSSGSTSTITNSGSDPQLQTLMPGYTINDATVLQFDFVPASDQLEFQ
                     YVFGSEEYMEWVGLGFNDVFAFFLSGPNPSGGNYNNVNIALIPNTSIPVTIDNVNLNT
                     NSQYYVNGSSYIEYDGYTVTLTAQADVVQCANYHIKLAVGDCGDSSYDSAVFLKAGSF
                     TSGESYTMQAFNAWGSALHFYEGCSNYLV"
BASE COUNT         1401 a          883 c         1035 g         1373 t
ORIGIN      
        1 aggtgaaggc agaggttttt gctacacagg attatactat tacagtaact gtaaatgcgc
       61 ctcctccaac agggcttgca tctggagact atgtctggac cggagcaatc agtacggatt
      121 ggaacaactc tgccaactgg gttgctttta acggaagtaa cttttctata cctgggtcag
      181 ttccagcaac ttcgaacgat gtattattta caacttatgg aacaagctgc ggaatcacct
      241 cgcctgtaat ctcatcggcc tctggtaatt gcaaagatat tacgattgaa tcaggacata
      301 cggttgtgat gtcgaataat aagatcctta atgttcaggg aaattggacc aatgatggaa
      361 cctttactgc cggaaacggt actgttgttt tcaacggatc tgcgcaacaa actattctag
      421 caggtggaag tgctttcaac aatgttacct tcagtaatac tacttccggt actaccgata
      481 ttaacattgg agaaccaatg ataataaacg gatcagcaac atttacaaat ggggtggttt
      541 attatacagg cacaggatcg cttactttcg gaagttcagc tacttcgaac agtggtggta
      601 taaatagtta cgtggatggt gtggtatcga aaacaggaac aagcgcattt acttttgccg
      661 ttggagctgg cccggtatgg gcgccaatag gtattgcagc gccctctgtt aattcaatta
      721 ttacagctga ttacaatttc actgaaggac cgttgaactg ggcagctgca tatatgtgcg
      781 acgaaagtca gatgaaatac accagtagcg ttgagcactg ggatttgact accacatcag
      841 caacaccggc agttacttta tactggactg atgcaacacg aagcgggata accgacctta
      901 ctgatttagc ggttgcccac ttcaatggca catgttggga atataagggt ggaaccacat
      961 cgggagactt aagctgggga agtataactt caaacatcaa cttctcaagt tatagtcctg
     1021 taagcttcgg cagtaagcgt ggagtaaata agttgccggt tgagttgacc caattcggcg
     1081 caaggtgtgg cgatgatgga atccttatta cgtggcagac agcatcagag actaacaacg
     1141 actattttac tctggaaaag tcagaaaatg cggttgactt cattcaaata gccgaaattc
     1201 aaggtgcagg aaatagtgtt gatttgattg aataccattt tttggatgag cgatatgtta
     1261 gcggaacttt ctattatcgt ttgaaacaga ccgattttga cgggagttcg aaatactcaa
     1321 atattgtaat agctaactgt tatgcgaacg gctcagccaa tccggttgtc atcgcatatc
     1381 ccaacccgtt caacgatgag ctgagtgtga ctgcatggaa cctgcctgaa ggagaaatta
     1441 ctttgacttt aaccgattta tttggcaaag actactatcg caaggaattg aaaacagata
     1501 aaggagaatt ttcaattacg ctttttctta aagatattgc ccctgctgta tatttattga
     1561 aaataaccaa aggggagttt acccggacaa taaaagtggt aaaatagcat ctaattattg
     1621 ttttaacttt ggcgggtaat ttttagaatt cttggatagt gtttgtatgc aagagcgata
     1681 atttttccac tatgaaagaa ctgtggaaca gactatacgg tgatgcaagc gattgctcat
     1741 ataacgaaat gataggttta ctctggttgt attcagagta taacagaaac aatccttata
     1801 aaaagcccaa atattggtac cgcgaagcag ttgtgtatgc attgtatgtc gatttgtttg
     1861 ctggtgactt caaaacactc atttcgaagc taccctatct gcaaaaatta ggtgtaaact
     1921 gcctctggct tttaccggtt ctcgactcac caatgaaaga tgcaggcttc gatgtgagaa
     1981 attacaatgc tatccggggg gatctgctca ctgctcctga aaaccaaaat gaatttgatg
     2041 attttctgga tgccgcgcac tcttcgggta tcaatgttat attcgatata gcaataaacc
     2101 acacatcaaa tacccatcca tggtttcagg atgctttgtc ttcaacgcaa agtcgctacc
     2161 atgattacta tatttggaat aaggatacgg aattgtataa ggaatgtcgt ttaatttttg
     2221 aagggatgtg tccctgtaat tgggaaaaag caggggagga gtattatttc cacagatttt
     2281 ttgaatttca gcccgatttg aattacaaaa acccacgtgt tttcattgaa atggttaaaa
     2341 ttttcttatt ctggctgaaa aaaggtgtag atgggttcag gctcgatgcc attccttact
     2401 tgtggaaaac agagggtacc gattgcgaaa accttccgga aacgcatctc attataaaac
     2461 tttttagggc aataattgat atggttgccc ctgaaacact gcttctggca gaagcctgtc
     2521 aacctccaaa ggaagtggtt aaatatatgg gtgatggaga tgaatgccat gcaggctacc
     2581 atttccccct tatgcctcag atttttaaag caatagcgat gcaaagcagt gaaccggtaa
     2641 ttaatgtact gaaacccgaa aatacacctg ctttgcccga atcgtgccaa tggtttttat
     2701 ttcttagact tcacgatgag ttgagcctcg aaaaggtgta tgttaacgaa gatgacagag
     2761 agtttttgca taagttctat tgcaaaaaaa atgaatggga cttccggttg gggcagggta
     2821 tttcggcgag actatcggag ctgatggacc atgatccaaa taaggtgttg ctgctttatt
     2881 caattatgtt cagcttgcag gggacacctc tgatatatta tggcgatgag tttggaaaat
     2941 caaacgattt agcatattat ttaaagaaaa agactgaaac cggatatgct gattcacgaa
     3001 acatggtccg cggttatgta gaatgggaga aaacccttac tgaaattgcc cgtgaaggtt
     3061 ccatagaaaa tgtactattc catggtatca gcaatatgct ttctgtacgg cagaaaatag
     3121 gcattttcgg aacagcctca atcgaaatgc tgaattttga gtgcaagata ctttcctact
     3181 ggagaacaat tggtaataat aagatattga taattaataa catatccgga aatgatgtga
     3241 aagttgaact gccattaaga gtaaatagta aatatgattt attgggcaat accctggcta
     3301 tttcgaaaaa aaccttgtta atgaaacccc attcatatca ttggattgca gtagaataat
     3361 ttttattact atatttacag aaaaaagcaa cgaattgttg aaaaaaacgt caattcaaaa
     3421 aagtaatatt atgaggatat catttatact tgcaatcctt ttgctttcct ttttacaaac
     3481 tgtgcagagc caaacattaa gttcggatta tcacgacccg gacttgttgc tattgaatca
     3541 ggaaattcat tactcgacat ttgcattagt gcagggggaa agcaccgaac taggtgtacc
     3601 tgaatgtagc agagattata ctaatagaaa ttactatgca cgttttcttc agtcttatca
     3661 aagtgcagtt caggtaattg cgcggtttga caatcaaaca aggttcggga tggctttgta
     3721 tctggtcgat ggagagcagt atcatgaatt gagatgtgat atttttgaca gcagcgaagc
     3781 atcgcttatt atgactaccg ccgaaaaccc cgaaggattg gatgttattg tgcgtctttg
     3841 ggtactcgat gatgttatac ctcagcaggt tggcttatgt gccctactcg tttcagaccc
     3901 accgggagag ccgaaactta tgacggtgaa cacaacaact tatactgttt cgcaattggt
     3961 taccgatgtt cttgttaccg gatgtctgca agcatcgaat gttacttttc agggtggagc
     4021 taccagtatt ggatacttct ctaatgccac acccggactg gattttgccg aaggaattgt
     4081 tatgagtaca ggtagtgtgc ttgatgctcc cggacccaat tcatcaggaa gcacttctac
     4141 cattaccaat agtggttccg accctcaact acaaaccctg atgccgggat acacaatcaa
     4201 tgatgctacc gtgcttcagt tcgactttgt cccggcttct gaccagcttg aatttcagta
     4261 tgtatttggt tcggaggaat atatggaatg ggtgggttta ggttttaacg acgtatttgc
     4321 ttttttctta tctggaccca atcccagcgg aggtaactat aacaacgtga atatagcatt
     4381 gattcctaat accagcattc ctgtaaccat cgacaatgtt aacctgaaca cgaactcaca
     4441 gtattatgtt aatggcagct cttatattga atatgatgga tatactgtaa ccctgactgc
     4501 acaggctgat gtggtgcaat gcgccaatta tcacataaaa ctggcagtag gcgattgtgg
     4561 agatagttca tacgactctg ccgtatttct taaagcagga agtttcactt caggcgaaag
     4621 ctatactatg caggccttca atgcatgggg ttctgcattg catttttatg aaggctgtag
     4681 caattatctc gt
//
LOCUS       DAOOQP010000174         4429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_23428_length_4429_cov_6.808871, whole genome shotgun sequence.
ACCESSION   DAOOQP010000174 DAOOQP010000000
VERSION     DAOOQP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23428_length_4429_cov_6.808871"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="PLK75_12305"
     CDS             <1..1467
                     /locus_tag="PLK75_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY40071.1"
                     /translation="GVVNIITKSASSDTEALLYADGGSYNSYHGGLSLQIPVNKNVKV
                     YSLTDWNESDGLPLLLPTSRVGKMAYSHLSSIQRIAFRPTDKLELYLSSRLTKTNMLW
                     QNLINPSHLFMPDAGLSYRIGSRAVVNAKLAYTNWYSKTNNEQNRLLRPELWINYTIS
                     EKNHLVAGTDFSASYFARTSVSGHSRNDFGFYIQDEHRFNETLIAQASLRYDKVQHVA
                     GVISPKLSLLYSPIQSIRLRAVAGRGFHAPGLMELYELGYGHGGTAYRFGNPDLKPEY
                     NTNFSLGVEWNHADKLVAGITGFYSIIDNMIVPVYAGPWEQNPAVNVWMRQNILKANI
                     ATGEFSLRWQFMSNYGVFLNYTYSQNTHTDASQQLPYQPGSSAGIRLFGVQHVSKKLS
                     IEEYISVKAVHGRSAWNWKPSTGASSDDPNGLITELADYQKLDAGISLQYDKRYEVFF
                     NVYNILGQDIQNLDDALTQIDGEPWFNLGVKLRFSGTK"
     gene            complement(1481..4066)
                     /locus_tag="PLK75_12310"
     CDS             complement(1481..4066)
                     /locus_tag="PLK75_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOY40072.1"
                     /translation="MKKILFIVTVLAFASCNNNKNSFDSANYKQVDTDFEEVKKLAVH
                     RNDALFTVFEQQLNDEEIRYLKFLYAYMPLSDIADLDGAFFLAQVRTALEARGQFSWG
                     KTIPDDIFRHFVLPYRVNNEFPDSARQVFYQELKPRIENLSMHDAALEVNHWCHEKVN
                     YRGTDERTISPLDAVRSGFGRCGEESTFAVTALRSVGIPARQVYTPRWAHCDDNHAWV
                     EVWVDGKWYFMGACEPEADLNRGWFEAPATRAMLVHTRVFGKYTETENQMITKKKYSI
                     INVIDTYIKPVQYVVLVKDPSGAVVENAEVQFGLYNYAEFYPIASLNTNSKGLCSLNT
                     NHGTLLVSATKDNRTAYAAFRVDEDKSADTLVLILAPFTPAAFEENIDIIVPVESATA
                     KHNTEDDARNQCRVKEEDSLRNAVAATFPDDKLIFVFAETRDLNSMDIGEHIKMSCGN
                     YSEIMDFIDKAAAIDKEKCLGMLTILNEKDHRDARSNILLDHFNHSTNNGYSNDIYLN
                     YVLNPRISTEKMKAYRSYLQAQFSTGFADSCRSNPGLVADMVKKQIAIDDEWNYYGVA
                     ITPVGVHTMKIADKRSRNIYFVAVCRSFGIAARLNPVTYIPEYYFGNSWKEVLFDAPE
                     ETGCEKSASLVLKSEKKALKYYADFTIAHCENGKFTTLDYGWDTPLSELSMPLQLRPG
                     YYMLMSGKRLQNGDVLVWRKFFSVETGKTLSVDIIVRNEINKNKVIGKITNFSKIAGA
                     KQADYYLTVWIDTGKEPGKHILQELADNRNSFDNAPLAINLQCVNSYKSDANIGKYSL
                     PASCTFTQISPNPDFSIIALDKPATKSELPVVALCNAKGEILYYQSGYKVGMANQILN
                     LISGK"
     gene            complement(4191..>4429)
                     /locus_tag="PLK75_12315"
     CDS             complement(4191..>4429)
                     /locus_tag="PLK75_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40073.1"
                     /translation="PFDRTEYYNRVDYGITTGLGAAVPIGEKILLSFEIRNNTGIYTI
                     NKIIEDYYYPPIKTSSTNLLIGFSYKFGMRETIK"
BASE COUNT         1177 a          982 c         1014 g         1256 t
ORIGIN      
        1 ggtgtggtaa acatcattac aaaaagtgca tcttccgata ccgaagcctt gctgtatgcc
       61 gatggaggta gttataactc ttatcatggc ggattatcct tgcaaattcc ggtcaacaaa
      121 aacgtgaagg tatattcact caccgactgg aatgaatctg atggactgcc attactgctg
      181 cccacgagcc gtgtcggcaa aatggcctat tcccacctga gcagcattca acgcattgca
      241 ttcagaccaa ccgataaact tgaactctat ctcagtagca gattaaccaa gaccaacatg
      301 ttgtggcaaa atctgattaa ccccagccat ttgtttatgc ccgatgcagg cttgagttat
      361 cggataggaa gtcgtgctgt tgttaatgca aaattggctt acacgaactg gtacagcaaa
      421 acgaacaatg aacagaaccg tttattgcgg ccggaattgt ggataaatta caccatttca
      481 gaaaagaatc acctggttgc aggaactgat ttctcagcta gttattttgc ccgaacctcc
      541 gtgtcggggc atagtcgcaa cgattttggt ttttacattc aggatgaaca ccgttttaac
      601 gaaaccctga tagcacaggc ttcgcttcgt tacgacaagg tgcagcatgt tgccggggtt
      661 atcagtccca aactatccct gttatacagc ccgattcaaa gtattcgtct gcgtgctgtg
      721 gctggcaggg gatttcacgc gcccggcctg atggaattgt atgaattggg ttatgggcat
      781 ggaggaacag cctatcgttt tggtaacccc gatttaaaac ccgagtataa caccaatttt
      841 tcgcttggtg tggaatggaa ccatgccgat aaactggtag ccggaattac cggcttttat
      901 agtattatcg ataatatgat tgttccggtt tatgccggcc cgtgggagca aaacccggcg
      961 gtaaatgtat ggatgcgaca gaacattctc aaggctaata tagcaacagg ggagttcagt
     1021 cttcgttggc agttcatgag caattatggt gtgtttttaa attataccta ttcgcaaaac
     1081 acacacaccg atgcaagcca gcaattaccg tatcaaccgg gaagttcggc tggcatacgt
     1141 ctgtttggag ttcagcatgt atctaaaaaa ctcagcatcg aagaatacat cagtgtaaag
     1201 gctgtgcacg gacgaagtgc gtggaactgg aagccttcca caggagcaag ttctgatgat
     1261 cccaatggct taattactga gttagccgat taccagaaac tcgatgcagg catttcgttg
     1321 cagtacgaca agcgctacga agtatttttc aatgtatata atatccttgg gcaggatatt
     1381 caaaaccttg atgatgcact gacccagatt gacggagaac cctggtttaa cttgggtgta
     1441 aagctccggt tttcgggcac aaaataagga attttcgggt ttattttccc gaaatcaggt
     1501 tcagtatctg atttgccatg cctactttat atccgctctg atagtataat atttcacctt
     1561 tggcattgca tagtgccacc accggaagtt cggattttgt tgccggttta tcaagagcaa
     1621 tgattgaaaa atcaggattg ggcgaaatct gcgtaaatgt acacgaggca ggaaggctgt
     1681 atttcccaat attcgcgtcg gatttatagc tgttaacaca ttgcaggtta atcgctaacg
     1741 gggcattgtc gaaactgttg cggttatcgg ctaattcctg caaaatatgc ttgccgggct
     1801 cctttccggt gtcgatccag actgtgagat aatagtcagc ttgtttagcc ccggcaattt
     1861 tgctgaagtt ggtaatttta cctatcacct tgtttttatt tatttcgtta cgaacaataa
     1921 tgtccacact cagggttttg ccggtttcca cactgaaaaa tttacgccat accagtacat
     1981 cgccattctg caagcgcttg cccgacataa gcatgtagta gccggggcga agctgtaaag
     2041 gcatcgacag ttctgataat ggtgtgtccc aaccatagtc aagtgtggta aactttccgt
     2101 tttcgcagtg tgcaatggtg aagtctgcgt aatatttcaa tgcctttttt tcggatttca
     2161 gaaccagaga agccgatttt tcgcagccgg tttcttcggg tgcatcaaaa agaacttctt
     2221 tccagctgtt gccaaaataa tattccggaa tataggtcac aggattcaac ctggcagcta
     2281 tcccaaagct ccggcacacg gccacaaaat agatgttccg gctgcgctta tcggcaattt
     2341 tcatcgtgtg cacgcccacg ggagttattg caacgccata gtaattccat tcatcgtcaa
     2401 tggcaatctg tttttttacc atgtcagcca ctaggccggg attgctccgg cacgaatcgg
     2461 caaagcctgt actaaactgc gcctgcagat agcttcggta ggctttcatt ttttcggtcg
     2521 aaatgcgggg atttaacacg tagtttagat agatgtcgtt gctatacccg ttgtttgttg
     2581 aatgattgaa atggtcaagc aatatattgc tgcgtgcatc gcgatggtct ttttcgttga
     2641 gaatggtcaa catgccaaga catttttctt tatcaatggc agccgcctta tcgataaaat
     2701 ccatgatttc ggagtagttg ccgcagctca ttttaatgtg ttccccaata tccattgaat
     2761 tcaggtcgcg ggtttcggca aaaacaaaaa tcagtttgtc atcggggaat gttgcagcaa
     2821 cagcatttct cagagaatct tcctccttta cccggcattg gttgcgggcg tcatcttcgg
     2881 tgttgtgctt ggctgtagcc gactcaaccg gaacgattat gtcgatattt tcctcaaatg
     2941 cagcaggggt aaacggggcc agaatcaaca ctaaagtatc agcgctttta tcttcatcta
     3001 ccctgaaagc agcataggcc gtacggttgt cttttgttgc cgaaaccaga agtgtgccgt
     3061 gattggtgtt caggctgcac aatcccttgg aattggtgtt caggctggcg attggataaa
     3121 attcggcata attgtataac ccgaattgga cttcagcatt ttcaacaact gcgccggacg
     3181 gatcttttac caaaacaaca tattgcaccg gtttaatgta ggtgtcaatc acattgataa
     3241 tggaatattt ctttttggtt atcatctggt tttccgtttc ggtgtacttg ccaaaaacac
     3301 gggtgtgtac cagcatggcg cgggtagccg gtgcttcgaa ccaaccccgg ttgaggtccg
     3361 cctcaggctc gcaggctccc ataaaatacc acttgccgtc gacccagacc tcaacccatg
     3421 catggttgtc gtcgcagtgt gcccagcgcg gggtgtaaac ctgccgggca ggaataccca
     3481 ccgaacggag ggcagtaacg gcaaaagtag attcttcgcc acagcggccg aatccggagc
     3541 gaacggcatc gagtgggctg atggtgcgct cgtcggtacc ccggtaattc actttttcgt
     3601 gacaccagtg gttcacttca agagccgcat cgtgcatgct taaattttca attcttggct
     3661 tcaattcctg gtaaaatacc tgacgggccg aatcgggaaa ctcattattc acccggtagg
     3721 gtagcacaaa atggcggaaa atatcatcgg ggatggtttt accccacgaa aactgcccgc
     3781 gggcttcgag tgcagtccga acctgggcaa gaaagaacgc tccatcgagg tcggctatgt
     3841 ccgacaaggg catataggca tataaaaact tcaggtagcg gatttcttcg tcgttcagct
     3901 gttgttcgaa tactgtaaac agcgcatcgt tacggtgcac agcaagtttt ttcacctctt
     3961 cgaaatcggt atctacctgt ttgtaattcg ctgaatcgaa ggagttcttg ttgttgttgc
     4021 aagacgcgaa tgcaagtact gtaactataa aaagaatttt tttcattttt cgattgaatt
     4081 ttgcccaaaa aaagttaact gaattatata cacaaatcta aaactttata ctgagagaaa
     4141 aaatacatcg agctgtcgat gcagcaagga ttgaattact gtatcaagat ttatttaatt
     4201 gtctccctca tcccaaactt atacgaaaaa ccaattaata aattcgttga ggaggtttta
     4261 atgggtggat aatagtagtc ctctattatt ttattaatcg tataaatgcc cgtattgttc
     4321 cttatttcaa aagaaagtaa tattttttct ccaattggta ccgcagctcc cagccctgta
     4381 gtaataccat agtcaacgcg attataatac tctgtcctgt caaaaggag
//
LOCUS       DAOOQP010000175         4367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_23836_length_4367_cov_6.795918, whole genome shotgun sequence.
ACCESSION   DAOOQP010000175 DAOOQP010000000
VERSION     DAOOQP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23836_length_4367_cov_6.795918"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2606)
                     /locus_tag="PLK75_12320"
     CDS             complement(<1..2606)
                     /locus_tag="PLK75_12320"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOY40074.1"
                     /translation="MKKLFALLCMALIMKAVMGQTNYTRLSEHFEDETSLIRIRIDEP
                     LLQNPEGGYGYNLKTAIPFTSFSIGWQAGTNNYPAHTYTIVYKVHKPGLGWSQWKTDD
                     GYTRPGETATGAYFTDLLFGFDEWLHDSIEFYVHPPEGETITEMQLVLMDISSSIRPD
                     DEMHPITSEAKTCPEFPDIIPRSEWCGSYEECHNPTYTVVYRTPTHTVVHHGASPDSY
                     TDGYAIVRSYWNYHVNTNGWSDIGYNYLFDKYGNFFQGRHNPNLPNQDVHAAHAGYAN
                     TYSIGLNFLGNSDAVNTAPTEPQIQKCCEFLAWWYDYKGFDPLSSASILNQAGTEWIT
                     LPRICGHKDVNPGGTTCPGNTLYALLPEIRTRTNQVITDCTTPTDQDPPSTAITTDRL
                     WYNSSFQAAFSDADNPGGTGVKYSFYQIMDYNGTEWRANASTGFFNDNFSTAIHPDWT
                     NNSGTWAINSGHLKQSNETSTNTNLYASLSQQSGNIYLYHWQQKISGSGSDRRSGMHF
                     FCSDPTGTGRGNSYMVYLRADANTVQIYKYIDNSYSAAGAWYITEDFIIDPDIWYDVK
                     VILNTITGEISVYVNDTLAASATDASPLASGTAISLRTGGCQTEYDDVKVYTSRGNSV
                     TVVPANTANADIRYQSPSHSQEAGRIRSLLVDNAGNWSESVSKNVFVDWDMPASELPA
                     PEPWYTDDFQVNINDLDNLSGVEKGFYSVSDFDGTRWGSNPERGFVSNSFDQTLGTEW
                     TQQTGSWSIQSGALTQTDETLSNTNLWAYIRHDLSNRYLYEFDMKIDGSNANKRVGFH
                     FFSDNPSLPNRGNGYFVWFRLQSQDLEFYKVTDDVFSLEKYYDINIVAGQWYHISIVY
                     DRITGETYVYMN"
     gene            2841..3590
                     /locus_tag="PLK75_12325"
     CDS             2841..3590
                     /locus_tag="PLK75_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOY40075.1"
                     /translation="MPVLVEVCLESVQSAVIAEKAGAHRIELCSALDLGGITPSQGLI
                     RSVCEAVSIPVFVLIRPRQGDFIYNSTELSLMNRDIELAIEAGAKGIVSGVLDATGCV
                     DSKATQQLIRASGGLPFTFHRAFDRTNNPMSALSAIIELGADRILTSGQASNVKAGSE
                     LIRSLIDKAANQITIMPGGGLTPENVGCILQTGATEIHLSARAPVPGSTSQIVNSVRF
                     NQSIAGENLHYETSETTLKQLFEQINKIQHS"
     gene            3587..>4367
                     /locus_tag="PLK75_12330"
     CDS             3587..>4367
                     /locus_tag="PLK75_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY40076.1"
                     /translation="MKKIPQIILIIIFSVALASSCKVGKSMNLHKSNANTFIVDDKKP
                     KVSGTNEDSCDMIILRNGEEISAKVLEIGIHEIKFKKCDNLNGPTISILKNDVFMIKY
                     SNGTKEVMKSTDNFSTDNSDKTATIPCNINANTFDIGIEGSPSLIYLRGLIDYNYEPS
                     LGFSGGISFQYNFPKIISIRTNVSFERKGAIRRITYTDIMGETISELVEHSNFNYITL
                     PILIKASLGKHVKYFITAGAFGGYLLNAEINYNIQVTTNSGD"
BASE COUNT         1238 a          957 c          949 g         1223 t
ORIGIN      
        1 taattcatgt acacataggt ttcgccggta atacggtcat aaacaatgct tatgtgatac
       61 cattgacccg ctacaatatt tatatcgtag tatttttcaa gcgaaaaaac atcgtcggtt
      121 actttataaa actcaaggtc ctgactttgc aggcggaacc agacgaaata gccgttgcct
      181 cggtttggca agcttggatt atcactgaaa aagtgaaatc ctacccgttt atttgcattg
      241 gaaccatcta ttttcatgtc gaattcatac aggtaacggt tcgacaaatc gtggcggata
      301 taggcccata agttggtatt actcaatgtt tcatcggttt gagtcaaagc acccgactga
      361 atcgaccaac taccggtctg ctgcgtccat tcggtgccga gggtctgatc gaagctattc
      421 gaaacaaacc ccctttcggg attcgaaccc cagcgtgttc catcaaaatc agacaccgaa
      481 taaaaacctt tttctactcc gctcaggtta tcaagatcgt ttatgtttac ctgaaaatca
      541 tcagtatacc agggttcggg tgcagggagt tcgcttgcag gcatatccca atcgacaaat
      601 acgtttttcg aaacactttc cgaccagttc cctgcattgt caaccaacaa actgcgaatc
      661 ctacccgctt cctgggagtg cgacgggctt tgatagcgga tgtcggcatt cgcggtattg
      721 gccggaacca ccgtaacgga attaccccgc gaagtgtaca ctttcacatc gtcgtattcg
      781 gtttggcaac cgcctgtgcg taacgaaatg gcagttcccg aggccaaagg ggacgcatcg
      841 gttgccgaag cggcaagggt atcattaaca taaaccgaaa tctcaccggt aatggtattg
      901 agaataactt ttacatcata ccagatgtcc ggatcaatta taaaatcctc ggtaatatac
      961 catgcaccgg ctgccgaata agagttgtcg atatatttgt aaatctgcac tgtattggca
     1021 tctgcccgca gatataccat ataggaattt cctcgcccgg tacccgtggg gtcactgcaa
     1081 aaaaagtgca tacccgaacg gcggtcgctg ccacttcccg agattttctg ctgccagtga
     1141 tacagatata tgtttcctga ttgctggcta aggctggcat ataagtttgt atttgtgctg
     1201 gtttcattcg attgtttcag gtgaccggag ttgattgccc aggtgcctga attatttgtc
     1261 cagtcagggt gaatggccgt tgaaaagtta tcgttgaaga aacccgtcga tgcattggca
     1321 cgccattcgg ttccgttata gtccattatt tgataaaaac tgtatttcac tccggttccg
     1381 cccggattat cggcatcgga aaacgcagcc tgaaagctgc tgttgtacca taacctatcg
     1441 gttgtgattg cagttgatgg gggatcctga tcggttggcg ttgtgcagtc ggtaataacc
     1501 tgatttgtcc gggttctgat ttcaggtagc agagcatata acgtgttccc cggacaggtt
     1561 gtccctccag ggttaacatc cttgtgccca caaatccggg gcaaggtaat ccattcggtt
     1621 ccggcctgat tgagaatact tgctgaactc agcggatcaa agcctttgta atcgtaccac
     1681 caggccagaa attcacagca cttctgaatc tgcggctcag tcggggcagt gtttaccgca
     1741 tcggagttac ctaaaaagtt caacccgatg gaataggtat tggcataacc tgcatgggca
     1801 gcatggacat cctgattcgg caggttcggg ttgtgccttc cttgaaagaa gttaccatat
     1861 ttatcaaaca aatagttata accaatatca gaccacccat tggtgttcac atgatagttc
     1921 cagtaacttc gtacaatagc atatccatca gtgtatgaat cggggctggc tccgtggtga
     1981 accaccgtat gtgtgggtgt gcggtaaaca accgtgtagg tcgggttatg acattcttca
     2041 tacgatccgc accactcgga acggggaatg atatcaggaa attcaggaca tgtttttgct
     2101 tccgaagtta tggggtgcat ttcgtcatca ggtcgtatgg aactacttat atccattaac
     2161 accaattgca tttcggttat ggtttcccct tcgggtggat gaacatagaa ttcaatagaa
     2221 tcgtgaagcc attcgtcgaa accaaaaagt aaatcagtaa aatacgctcc ggtggcggtt
     2281 tctcccggtc gtgtgtaacc atcatcggtt ttccattgcg accaacccaa accaggttta
     2341 tgaaccttgt acacaatagt atatgtgtgt gccggataat tattggtacc cgcctgccag
     2401 cctatcgaaa acgaggtaaa agggatggcc gtctttaggt tataaccgta accaccttcg
     2461 ggattttgta aaagcggttc gtcaatccga attcgtatta aagatgtttc atcttcgaaa
     2521 tgctcgctga gccgggtgta attcgtctgc cccatgacgg ctttcattat taaagccatg
     2581 cataacaaag caaagagctt tttcatagtc catataattt acaatgcaat ttacgaaatt
     2641 agaattgaat ttcaaaagtc tttcaccaaa agtcctcgtt tgcaacgagg acttaattat
     2701 cggtgcattt ttatgcactg tattcatcgc attgcaaatg ccaagtgtaa acccgacctc
     2761 gttgcaaacg aggtcgaggt gtagttatat tagtagaaaa atgtacattt ctttctatct
     2821 ttatccaaaa ttttatcgtc atgcctgtac ttgtcgaagt atgtcttgag tcggttcaat
     2881 ctgctgtaat tgccgaaaaa gcaggtgccc accggataga gttgtgttca gccctcgacc
     2941 tgggtggcat cacgccatcg cagggactga ttcgttcggt atgtgaggca gtgtcaattc
     3001 cggtatttgt gctgatacgt ccgcgtcagg gagattttat ctataacagc actgaacttt
     3061 cgctgatgaa tcgcgatatc gagctggcca tcgaagccgg cgccaaggga atcgtttcgg
     3121 gtgtcctcga tgccacaggc tgtgtagata gtaaagccac gcaacaactt atccgggcca
     3181 gcggaggatt accctttaca tttcaccgcg cattcgatcg cacaaacaac ccaatgtcgg
     3241 ccctatcagc tattattgag cttggggctg acagaatact tacatcagga caagcgtcca
     3301 atgtaaaagc gggtagtgaa ctgattcgtt cgctcatcga taaggctgca aaccagatta
     3361 ccatcatgcc cgggggtgga cttacgcccg aaaatgtcgg ttgcatcctt cagaccggag
     3421 ctactgaaat acatctttca gcacgggctc cggtgcctgg ctccacaagt caaatcgtaa
     3481 actcagttag gttcaaccag agcattgcag gcgaaaacct gcactacgaa acttctgaaa
     3541 ccacattaaa acaacttttc gaacaaataa ataaaattca acactcatga aaaagatacc
     3601 acaaattatt ttaattatca ttttttcagt agcactggct tcatcatgca aggtgggaaa
     3661 aagcatgaat cttcacaaat cgaatgctaa tacttttatt gttgatgaca agaaaccgaa
     3721 agtatctgga acgaatgaag attcctgcga catgataatt ttaagaaatg gtgaagaaat
     3781 ttcggcaaaa gttttagaaa ttggaattca cgaaattaaa ttcaaaaaat gtgacaattt
     3841 gaatggtcca acgatttcaa ttcttaaaaa cgatgttttt atgattaaat attccaatgg
     3901 aacaaaagag gtaatgaaat caaccgacaa cttttctact gataattcag ataaaacggc
     3961 aacaattcct tgtaatatca acgcaaatac atttgacatc ggaattgaag gaagcccaag
     4021 cctgatttat ttgagggggt taatagatta taattatgaa ccctctttgg gcttttcagg
     4081 cggcatttca tttcaatata attttcctaa aattatttca atcagaacaa atgtttcctt
     4141 tgaaaggaaa ggtgcaatac gtcgtatcac atatactgat atcatgggag aaacaatcag
     4201 tgagctagta gagcactcga attttaatta tataacactc cccattctta ttaaagcttc
     4261 gctgggtaaa catgtcaagt attttattac cgccggtgct tttgggggct atcttttaaa
     4321 tgccgaaata aattataata ttcaggtaac aacaaatagt ggcgaca
//
LOCUS       DAOOQP010000176         4352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_23938_length_4352_cov_4.599488, whole genome shotgun sequence.
ACCESSION   DAOOQP010000176 DAOOQP010000000
VERSION     DAOOQP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23938_length_4352_cov_4.599488"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..859
                     /locus_tag="PLK75_12335"
     CDS             104..859
                     /locus_tag="PLK75_12335"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOY40077.1"
                     /translation="MQDLGKIINDYMPISLSEMDSVKLLNRLDKKYILPSSKLIEILR
                     ELIQDYYILQINDVRNCRYTTVYYDTDDFMLFRQHQNGKMNRYKVRFRRYEDSDVNFF
                     ELKFKNNKSRTIKERLETNTFKPELTRKKTEFLEKKTQFIAAEFKPVITIHYRRMTLV
                     SKEMNERMTIDTDLWFENNNARSDYNNLVIIETKQGGNIHSYASEVLRNYHIHGFSIS
                     KYCLGIVSLYNEVKKNRFKVKQMKINKICYECN"
     gene            846..1634
                     /locus_tag="PLK75_12340"
     CDS             846..1634
                     /locus_tag="PLK75_12340"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOY40078.1"
                     /translation="MNVIKLIISITLFVFLVSPPQLCYSQSSENSEIIENTSDFSVET
                     DKAKDKDKKDDKSKKEKDIIKLNLHFFGNLGIDILTMLIIILLFYLPGTKNRENIFSL
                     IMFNITIFLLTFVLNDVKISMGAAFGLFAVFSMLRYRTAGISMKDMTYLFVFIALGLL
                     GAISLDYYELGIINGIVIMFIFLLDGGILLRRELSKRIQYEIIENIKPERYNELLSDL
                     KKRTGLDIHRVTIGRVDFLKDSASITVFYRSNKNKNALSENKTE"
     gene            1615..2298
                     /locus_tag="PLK75_12345"
     CDS             1615..2298
                     /locus_tag="PLK75_12345"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOY40079.1"
                     /translation="MKIKLNKILLLLANVLFAASLTGYSQINDAGLWLSLGAEKKIKQ
                     ALSVNYTAEFRLNENFTELGTIYNDFGLEYRFNKNLSVGAAYRFIAKKQPENYYSFRH
                     RFYIDLNYKRTVGIVRLSGRLRLQNHYEDVFSSEQGWYPETLLRGRVGAKFNIPYPVK
                     PYATVEVMTPLSGTELFTLEKIRYRAGLEYELNRMHSFDLFYMFQHDYGSRIPRNDFV
                     VGMGYYFTF"
     gene            2375..4102
                     /locus_tag="PLK75_12350"
     CDS             2375..4102
                     /locus_tag="PLK75_12350"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOY40080.1"
                     /translation="MKNFFIRIAVIVIVLSVHICNATIAETIVIAENMYAIDETKKLV
                     LVNQDVATINLQWPNQKTGIILNDFYTFSEEISTVQIGVAYQAVKSSVNYTIYFSQLP
                     IIRIYNQGPIYDDPKVLAEFIMTETDQTTISSFVGIELRGNTSLWWDKHNYLIEFWTD
                     AQGANTHDVSLLGMRSDDDWNLQSLYNEPLRIRSVSTNDLWMNFGSLYYQQIEPEAKP
                     GIVMKYAELFLNGRYKGIYAVSERVDRKLLQIKKYDEDDDEIRGELYKGTEWANGTLF
                     NYADDFDNQSAYWSGFEYEYPEELIDWYYLHDFVDFVVNSTNDEFYATYESKFDVQSA
                     IDYYILMNVSVAVDNRGKNIFIARYDAGEPYFYVPWDLDGVFGIMWDGTNTSPNAGLL
                     SNGLYDRMLCDCDPNGFNQRMKSRWLELRESEITHESIMNIFETNYNYLLTNGAYERE
                     QIAWTTFSYGQSKLDYISQWTTIRLDYLDMRFAEPCVPAGIHAAADDNDFAMYPNPAS
                     DKVYITTNSGRNYYSVVVNDYSGKTVLQSAIHTNPAVLDVSVLQPGLYIVQLISDNGT
                     AIRKLIVNR"
BASE COUNT         1387 a          775 c          904 g         1286 t
ORIGIN      
        1 atacctacga ttccaagggt ttgcgtacag aaaaaagaga atataacagt gatggttcac
       61 ttaagtctat ccgtaaaatt tcgtacgaat actattagca gaaatgcaag acttaggcaa
      121 aattatcaat gattacatgc ccatttccct gagtgagatg gactcagtga aacttttgaa
      181 caggttagat aagaaatata ttttaccaag cagcaaactc attgaaatat tgcgtgagct
      241 gattcaagat tattatatcc ttcagattaa tgatgtcaga aattgtcgtt acacaacggt
      301 atattacgat accgatgatt ttatgctttt tcgtcaacac cagaacggca aaatgaatcg
      361 gtataaagtc cgctttcgaa ggtatgagga ttcggatgtg aatttttttg aactgaagtt
      421 taaaaataat aaatccagaa ccattaaaga aagacttgag actaatacgt tcaaacctga
      481 gcttacaagg aaaaagacag aattcctcga aaaaaagact cagtttattg ctgcagagtt
      541 caaaccggtg attacaatcc actataggcg aatgacactg gtgagtaagg agatgaacga
      601 aaggatgact attgatacag atttatggtt cgagaacaac aatgcaagat ccgactataa
      661 caatctggtg attatagaaa ccaaacaggg tggtaatata cactcgtatg cctcagaggt
      721 gttgaggaat taccatattc acggtttctc cataagtaag tattgccttg gtattgtttc
      781 actgtataat gaagtgaaga aaaacaggtt taaagtaaag caaatgaaaa ttaataaaat
      841 ttgttatgaa tgtaattaaa ctaatcatca gtattacgtt gtttgttttt ttggtgtcac
      901 cgccgcagct ttgctattct caaagctccg aaaacagcga aatcatcgaa aatacatccg
      961 atttttcagt tgaaacagat aaggcaaaag ataaagacaa aaaagatgac aaatcgaaaa
     1021 aggagaaaga tataattaag ctgaatctcc atttttttgg aaatctgggc atcgatatac
     1081 tcacgatgct tataatcatt ctattgtttt atttaccggg cacaaaaaac cgtgaaaaca
     1141 ttttttcgct gatcatgttc aacataacga tttttctgct gacttttgtg ttgaacgatg
     1201 ttaaaatatc gatgggggct gcattcggac tgtttgcggt attcagtatg ttgcgctatc
     1261 gcacagccgg tatttcgatg aaagacatga cttatttatt cgtgttcata gcacttggct
     1321 tattgggagc aatcagcctt gattattacg aattaggtat tattaacggg attgtgatta
     1381 tgttcatttt tctcctcgac ggaggtatcc ttttgcgacg tgagctctcg aaacgaattc
     1441 aatacgaaat aatcgaaaat atcaagcctg agcgctataa tgaattgctg tccgacctga
     1501 aaaaacgaac aggcctcgat attcaccgcg ttacaattgg cagggtcgat tttcttaaag
     1561 attcagcaag tataacagtt ttctacagaa gtaataagaa caaaaacgca ctaagtgaaa
     1621 ataaaactga ataaaatact cctgctgctc gcaaatgttt tatttgcggc ttctctaaca
     1681 ggttacagcc agattaacga tgccggactg tggttgtcgc tgggagctga aaaaaaaata
     1741 aaacaggctc tctcggtaaa ctacactgct gaatttcgct tgaatgagaa ttttactgag
     1801 ttgggaacaa tttacaatga tttcggactg gaataccgat tcaacaaaaa tttaagcgtt
     1861 ggtgctgctt acagatttat tgccaaaaaa cagcccgaaa attattactc atttcgtcac
     1921 cgtttttaca ttgacctgaa ttacaaaaga actgttggta ttgtgcgttt atcaggtcgc
     1981 ctgaggctgc agaatcacta tgaagacgta ttttcgtcgg aacagggttg gtatcccgaa
     2041 acattattgc gcggtcgtgt aggggcgaag ttcaatatcc cataccctgt taagccctac
     2101 gcaacggtag aagtaatgac cccgttgtcg ggaacagaac tgtttacatt ggaaaaaatc
     2161 aggtacaggg caggtttgga atacgaactc aaccgaatgc actctttcga tttgttttat
     2221 atgttccagc acgattatgg ttcaaggata ccccgaaacg attttgtcgt tggtatggga
     2281 tattacttta ctttctgaga gtacgccttt tggggtagtt aattaaaaaa atattatttt
     2341 tacttgctcg tttttgtttt tgccgggtag aattatgaag aactttttca tcagaattgc
     2401 ggttatagtc attgtgttgt cggtgcacat ttgtaatgct acaatagctg aaacaattgt
     2461 tattgccgaa aatatgtatg caatcgatga aaccaaaaag ctggttttgg ttaatcagga
     2521 tgtggcaaca ataaatctgc aatggccaaa tcagaaaaca ggtataattc ttaacgattt
     2581 ttatactttt tcggaagaaa tatcaacggt tcaaatagga gttgcatatc aggctgttaa
     2641 aagcagcgta aactatacaa tatatttttc gcagcttccg ataattcgca tttataatca
     2701 aggacctata tatgatgatc ctaaggtact tgctgaattt ataatgaccg aaaccgacca
     2761 gacaactatt agttcatttg ttggtattga actacggggc aacacctcgt tatggtggga
     2821 taagcataat tatctgattg aattctggac tgatgcacag ggagcaaata ctcatgatgt
     2881 ttcgttgctt ggtatgcgca gcgacgatga ctggaatctg cagtcgctgt acaacgagcc
     2941 gctgcggatt cggtcggtca gcaccaacga tttatggatg aatttcggat cgctttatta
     3001 tcagcaaatc gaaccggaag ccaaacccgg tattgtgatg aaatacgccg agcttttcct
     3061 taatggacgt tacaagggta tttacgctgt aagcgaaagg gtcgaccgta aactccttca
     3121 gattaagaaa tatgatgaag atgacgatga aatcaggggg gagttgtata aaggtaccga
     3181 atgggcaaac ggaactttgt ttaactatgc cgacgatttc gataatcagt ctgcttattg
     3241 gagcggattt gagtatgaat atcccgagga gcttatcgat tggtactatc tgcacgattt
     3301 cgtcgacttt gttgttaatt ctacaaatga tgaattctat gccacatatg aatcgaagtt
     3361 tgatgtgcaa agtgctattg attactatat cctgatgaat gtatcagtgg ctgttgacaa
     3421 taggggtaaa aacattttca ttgcccgtta cgatgcagga gaaccctatt tttatgtccc
     3481 atgggatctt gatggtgtgt ttgggattat gtgggacgga acaaatacaa gccccaatgc
     3541 aggtctcctt tcaaacggtt tatacgacag aatgctttgc gattgcgatc ccaacggatt
     3601 taaccaacgc atgaagagcc gctggctcga attgagggag tccgaaatta cccatgaaag
     3661 tattatgaat atatttgaaa ccaactataa ttacttgctt acaaatggtg cttacgagcg
     3721 ggagcaaata gcctggacca catttagtta cggacaatca aagctcgatt atatctcaca
     3781 atggaccaca ataaggctcg attaccttga tatgcgtttt gcggagccat gcgtacctgc
     3841 tggcattcat gcagcagctg atgataacga ttttgcaatg taccccaatc cggcaagcga
     3901 taaagtgtat attacaacca actcaggcag aaattattat agcgttgttg ttaacgatta
     3961 ttccggaaaa actgtattgc agtcagcaat acataccaac cctgcagtgc ttgacgtttc
     4021 ggttctgcaa ccgggtttat acattgttca gcttatctca gacaatggaa cagcgatacg
     4081 taaactgatt gtaaaccgtt aattaagttt ttataatcag ccagactgac gtaaccgaat
     4141 attggtttga actaatagtt aatgattttt gccaaagcaa ctattaccca aaagatgtgc
     4201 tacaggaata ccttaagata cgcaagttaa agctaagcgt caacacccgg tactattcgc
     4261 taaacgatgc ttgctgcaat aggagtgagg ttctatttta tataatactc cccgaaaatt
     4321 ggaccactgg ttaagttgaa taaaaacaat ta
//
LOCUS       DAOOQP010000177         4307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_24257_length_4307_cov_4.707197, whole genome shotgun sequence.
ACCESSION   DAOOQP010000177 DAOOQP010000000
VERSION     DAOOQP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24257_length_4307_cov_4.707197"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLK75_12355"
     CDS             <1..1068
                     /locus_tag="PLK75_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40081.1"
                     /translation="ALGSWDYVVLQEQSQMPSFPPEQVETEVFPYATILCDSIRAANP
                     CTIPLFFMTWGRENGDQTNCPYYEPLCTYEGMQLRLRDSYVQMAVDNEAEVAPVGMVW
                     KTVRDSLQDTLVIYAGDSGGHPSLLGTYLAACTFYASMFHKSPVGALVPEGLEADDVL
                     AVQEYASMIVFDSLDTWQIDTLTVRADFEAEYLTKDILVYGYLQNWSVNADSCYWDFG
                     DGEFEWQYPYNPGQWDMVFHNFPEEDYYDICLTAYAGCKTDNYCETIYVFPQKIEDYT
                     KSSRHFSIYPNPTVNNISITLDHNIEIQSIQIVDITGRTCILNHDKTSQFYDVSLLTP
                     GFYTVIVKSEGAVYKGNFLKL"
     gene            1144..2391
                     /locus_tag="PLK75_12360"
     CDS             1144..2391
                     /locus_tag="PLK75_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY40082.1"
                     /translation="MIFLRTIRDSVYFAFSSLITNKLRTFLSLLGITIGIFSIISVMA
                     VLDSLERTIRDNVSSLGNNTVYIQKWPWTFGPEYPWWKYINRPVPSLREYEFIKDNSQ
                     LSDACAFIVSTSRTVEYEGKSIDNTGVIVSTPEYDRIWKIELSNGRYFSASETHTGRP
                     VCIIGAEISEELFRGIDPVGKNIKIGGNKVNIIGVIQREGEDMFSNSHDRAVLLPMNY
                     GRTIFDVKNESMDPFITVTAKEGVNIDELQAELNVLMRTIRRLRPMEEEDFALNRASM
                     ISQGLDSIFSIIDLAGIIIGGFAILVGGFGIANIMFVSVRERTRIIGIQKAIGAKQYL
                     ILIQFLSEAVVLCLIGGLFGLLLVWIGTLIAAGSVEDIKFTISATNIITGLLISVGIG
                     IVSGFAPARAAARLDPVDAINRV"
     gene            2452..3021
                     /locus_tag="PLK75_12365"
     CDS             2452..3021
                     /locus_tag="PLK75_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY40083.1"
                     /translation="MYLFFDTETTGLPKNWKAPVTDLNNWPRLVQLAYLLYDVSGNKI
                     SGGEYIVKPEGFIIPADAARVHGITTEKAMRDGTPIAIVLTDFFDLINRSNYLVAHNM
                     SFDENIMGAEFLRNGFNNPIPKKHKICTMEKTVDFCKIEGRYGYKWPKLSELYFKLFK
                     TGFDEAHNAAVDINATAKCFWELRRLGKI"
     gene            3148..>4307
                     /locus_tag="PLK75_12370"
     CDS             3148..>4307
                     /locus_tag="PLK75_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY40084.1"
                     /translation="MAILKSFRSILNGLFKKQKNPACFAPFNNMTIEKSGKVTVCCYN
                     RSLVLGKYPDESLNNIWFGNTRKLLIEQFLIDRAPSSCYHCLTNCLSTDSPESKIFCS
                     RAWNLSRFSDYPVQIEFILDNICNLDCIMCSQFCSTSSTNEVALDVEKVDYDHRFLKE
                     LEPFLKSGNFFVFSGGEPFLIPVYKEIWKYIHKENPKAKIYVQTNGTILTEEIKHIIE
                     TYKMEISVSIDSVEKNTYEQIRRNASFEKTFHNLEYFLNYAETYKKTITLRITPSKIN
                     AYEIPEVLTYCNSKNMLLGLGIIESPCYLAIWSLPSSELEIIINNYRFIYIDDKTDNP
                     VIRRNNIIFSSYVNLVNKYYDKKKYNEQNKEIILSNMIKACEDIEKNFITNLS"
BASE COUNT         1343 a          730 c          886 g         1348 t
ORIGIN      
        1 gcccttggat cgtgggacta tgtagttctt caggagcaaa gccagatgcc ttcatttccc
       61 cctgaacagg tcgagacaga agtttttccg tatgctacaa ttctttgcga cagcatcagg
      121 gctgccaatc cctgtactat tcctttgttt tttatgacct ggggacgtga gaatggagat
      181 cagactaact gcccatatta cgagcctctt tgcacttacg aaggaatgca gttacgcctg
      241 cgcgacagtt atgtgcagat ggcggttgac aatgaggcag aagtagcacc ggtgggaatg
      301 gtttggaaaa ccgtacgcga cagcctgcag gatactcttg tgatttatgc aggtgattct
      361 ggagggcatc cgtcattgtt aggtacatac cttgctgcat gtacgtttta tgcttctatg
      421 tttcataaaa gccctgtggg tgcattggtc cccgaaggac ttgaagcgga cgatgtgctg
      481 gcagtgcagg aatatgcaag catgattgtt ttcgacagcc tcgatacatg gcaaatagat
      541 accctaaccg tcagagccga tttcgaagct gagtatctta ccaaagatat tttggtgtac
      601 ggttatttgc agaactggag tgtgaatgcc gactcctgtt actgggattt tggtgatgga
      661 gaatttgaat ggcagtaccc ttacaatccc ggtcagtggg atatggtttt tcataatttc
      721 cccgaagaag attactatga tatttgcctg actgcgtatg cgggatgcaa aaccgacaat
      781 tattgtgaaa cgatatatgt gtttccacaa aaaattgaag attacacaaa aagcagccgg
      841 cattttagta tctatccaaa cccaactgta aataatattt caataacatt ggaccataat
      901 atcgaaatac aatcaataca aattgttgat attacgggga gaacatgtat tctcaaccac
      961 gataaaactt cacagtttta tgatgtaagt ttgttgacac cagggttcta tacagtcatt
     1021 gtgaaatcgg aaggggccgt ttataaggga aatttcctga aattatagac gctgcaacta
     1081 attttaacaa cttattaatt gacctgtttc gaataatgtt tatttttgtt caaaaccact
     1141 tacgtgatat ttttacgcac catacgcgat agtgtttatt ttgcttttag ttcgctgatt
     1201 acaaataaat tgcgaacatt tttatcgctt ttaggtatca ctattggcat ttttagtatt
     1261 atttcggtaa tggcggtgct tgattcactg gaaaggacaa tacgcgataa tgtaagctct
     1321 ttgggtaaca acacggtgta cattcagaaa tggccatgga catttgggcc agaatatccc
     1381 tggtggaaat atattaaccg gcctgtgcca agcttgcgcg aatatgaatt cattaaagac
     1441 aattcacagc tttccgatgc ttgtgcgttt attgtatcta cttcacgtac agttgaatac
     1501 gaaggaaagt caatagataa cacgggtgta attgtttcca cacctgaata tgacagaata
     1561 tggaagattg aactgagcaa tggccggtat ttcagtgcat cggaaacgca taccggcaga
     1621 ccggtttgca taattggtgc cgaaatttca gaggaattgt ttcgcggcat tgatccggtc
     1681 ggaaaaaaca taaaaattgg tggaaataaa gtaaacataa ttggtgtaat acaacgcgaa
     1741 ggtgaggata tgttcagcaa tagccacgac agagctgttc tgttaccaat gaattatgga
     1801 agaactattt ttgatgtgaa gaatgagtcg atggacccat ttattacggt tacagcaaaa
     1861 gaaggggtaa atattgatga acttcaggct gaacttaatg tgttgatgcg tacgattagg
     1921 cgactgaggc ctatggagga agaggatttt gctctcaaca gagccagtat gatttcgcag
     1981 ggccttgata gtattttcag tatcatcgat ctggcaggaa taattatcgg gggctttgct
     2041 attttagttg gtggatttgg gattgccaac atcatgtttg tttcggtaag ggagcgtacg
     2101 cgaattatcg gaatacaaaa agctattggg gctaagcaat accttattct aattcaattc
     2161 ctttcggaag cggttgtgct ttgtcttata ggaggtttgt ttggcctgtt gctggtttgg
     2221 ataggcactt taattgctgc tgggtcggta gaagatatta agtttaccat ttcagcaaca
     2281 aatattatta ccggtttgct gatttcggtt ggtattggta ttgtctcagg atttgccccg
     2341 gcacgtgcag cagcccgtct tgatccggtg gatgctatca acagggttta gttcaacttt
     2401 catgattatt ccagagaaag taatcgatta tacacaacat aaaacaatac catgtatcta
     2461 tttttcgaca cagaaaccac cggcttacca aaaaactgga aagcccctgt aaccgacctg
     2521 aataactggc cccgcctggt tcaacttgcc tatttacttt acgatgtcag cggaaataaa
     2581 atttcaggcg gagaatatat tgtgaaacct gaagggttca ttattcctgc cgatgcagca
     2641 agagtgcatg gtataaccac tgagaaagcc atgcgggatg gtactcccat tgctatagtg
     2701 cttactgatt ttttcgattt gataaaccgg agcaattatt tggttgcaca taatatgtcg
     2761 tttgatgaga atataatggg tgcagaattc cttcgaaatg gatttaacaa cccaatcccg
     2821 aaaaaacata aaatttgtac catggaaaaa actgttgatt tctgcaaaat cgaaggccgt
     2881 tatggatata agtggccaaa gctttcggaa ctttatttta aattattcaa aactggtttt
     2941 gatgaggcgc ataatgccgc tgtcgacatt aatgcaactg caaaatgttt ttgggaattg
     3001 agaaggttag gtaaaatatg aattttatgc ctgaattcat aacatgaatt ctttctcaat
     3061 tgttttatag aatatctttt cattaggcat gtttattttt gtttactttg tgagaatgct
     3121 tctttaaaaa tttatatcta tgtatgtatg gctatattaa aaagtttcag atccatactt
     3181 aacggattat ttaaaaaaca aaaaaatccc gcctgctttg caccatttaa taatatgact
     3241 attgaaaaaa gcggaaaagt tacggtgtgc tgctataata gatctttagt tcttggcaaa
     3301 taccccgatg agtcacttaa taatatttgg tttggcaata cgagaaagct tttaatagaa
     3361 caattcctaa tcgatagagc cccttcatca tgttaccatt gtttgactaa ctgtttaagc
     3421 actgattctc ccgaatcaaa gatattttgt tcaagggcat ggaacttatc gaggttttct
     3481 gattatccgg tacaaataga atttatactt gataatatct gcaatttgga ttgtatcatg
     3541 tgttctcaat tctgttcaac atcttcaacc aatgaagtcg ctttggatgt agaaaaagtt
     3601 gattatgacc ataggttttt aaaagagtta gaaccttttc ttaaaagtgg taattttttt
     3661 gttttttctg gtggagaacc atttttaatc ccagtttata aagaaatatg gaagtatata
     3721 cataaagaaa accccaaagc gaagatttat gttcaaacaa atggaactat tcttactgaa
     3781 gaaataaaac atattatcga aacctataag atggaaattt ctgtttcaat tgattctgtg
     3841 gaaaagaata catacgagca gattaggaga aatgcaagct ttgagaaaac tttccacaac
     3901 ctagaatact tcttgaatta cgctgaaaca tataagaaaa caattacatt acgaatcaca
     3961 ccgtcaaaaa ttaatgctta cgaaatacca gaagttctta catattgtaa cagcaagaat
     4021 atgcttttgg gccttggaat tattgaaagt ccatgttatc tggcaatatg gtcgttacca
     4081 tcatcagaac ttgaaataat cattaataat tatagattta tatatattga tgataaaact
     4141 gataaccctg ttattagaag aaataatatt attttctcaa gctatgttaa tttggtgaat
     4201 aaatattatg ataaaaagaa atataatgaa caaaataaag agattatact ctcgaacatg
     4261 ataaaagcat gtgaggatat tgaaaaaaac tttatcacta atctatc
//
LOCUS       DAOOQP010000178         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_25292_length_4174_cov_4.193251, whole genome shotgun sequence.
ACCESSION   DAOOQP010000178 DAOOQP010000000
VERSION     DAOOQP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25292_length_4174_cov_4.193251"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3484)
                     /locus_tag="PLK75_12375"
     CDS             complement(<1..3484)
                     /locus_tag="PLK75_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40085.1"
                     /translation="MKKIQITIIAGLLLLAANITFAQTYNLSNTTVNTCSGTLYDSGG
                     ASGNYGNNENITFVLCSNNGSTLSLHFTAFNLAANDNISIYNGVGGLNNLLASGSGTT
                     LNGQTINSLVDCITIVFTSNASGVASGFAATITCATLCQGYTTEITSNPPITDTDSMH
                     LDICPGDGVTFTALSTFPNNGSEGYNQTTANTHYYWELLDGTGLSIIDDDGVGLTTLS
                     YTFTNPGGYTLRLRSTDVEGCWNMGEANLRIRVSIPPHFNGTSLSPNPICPGQTVNLN
                     GVVHTTPWEITVTDTQIVQECIDDNVGDVQEVCFQVNSFAPGQTIQSATDLESACINM
                     DHSFIGDLYIWVVCPNGQEASLHEYYNCNGAYFGVPNHADNCQPGTGWDYCWSMSGTQ
                     LITSVCSSGSSVPAGTYLPLPSGSSFSSLIGCPINGEWCIKFIDNWGQDDGIIFTCDI
                     NFNDNILPQSLWGFENSYVLGNNSPDAHWDGNNMIDNIGSIGSAQPLLAGTNVPYTFY
                     ATDNFGCTYDTTITVTVRPITDPNCCQMPNPSAGTAAAICDVCYNLSANQPVAGNNGI
                     WTQVSGPGTATFTNNTLYNTQVCINNIYGTYQFRWTEYYQGNQACSSSDTVNITFNET
                     FDPTVTPIDDMCASHEPVQVQAVDFGTLTCPSCPAGSFDPASGIFTPENAGPGNYTIT
                     NTITGPCAGAGASSTTFTVFDELHIVNFNDQECIYGTTPVYVTEWDMEGVDGPFTGNY
                     LVNGVTTSGHFEETHTSATNYAYTITDVNGCTNIPISGFRDCNCPSAGTMTTLQLQIL
                     CQGTCTGDYVGHNGDQIMLGNSLFEFMIHNGNNVPLAYSATTNFCQNQIPGFSYNTTY
                     YISAIAGYDNNGDGHPELTGCYSIAVGTPVMWLQNPTPYAGTDRDTCGLLIRLNGNPV
                     PSGMIGYWSSDCDFFPVGGSSVSTPNMLTMTNLCQTCTFSWNIVNGQCTASDDVTVNF
                     RCTPNPYAGADVTVCGTAVDLNGNQSIPGSTITWSGNGVSFNPVSAPATTATIQTYGT
                     YQFTMTEQNGSCWSQDQVNVTFVQGPTPTVSMNHDTVCGVTYTLQVYNVTGEGIWRAY
                     DTLGIQITPTYVPPSNAYTPNATVIIPAYSGLFNTINFCWVETNDVNGVQCEAEACVE
                     VTFSREPSASVGPDN"
     gene            complement(3488..3928)
                     /locus_tag="PLK75_12380"
     CDS             complement(3488..3928)
                     /locus_tag="PLK75_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40086.1"
                     /translation="MKKIKLAGIFLILLISGVAVAQSSIEPDPRLKAIYSEDNLQQMK
                     INTPVQLDVLNFNLDHSWFIVEDAIFDKVKDSPYLYYVDPETGFQSENKVTSIDFSSV
                     NIALYWVDQQYDRRVFYRVGDTGIVLGFYSSKEMVTMYNQSKNQ"
BASE COUNT         1181 a          873 c          922 g         1198 t
ORIGIN      
        1 agttatcagg accaacgctt gcggaaggtt ccctcgaaaa agtaacttca acgcacgcct
       61 cagcttcgca ctgcacaccg ttcacatcgt ttgtctcaac ccagcagaaa ttaattgtat
      121 tgaacaatcc gctatatgcg gggatgataa ccgtagcatt gggtgtgtaa gcattagatg
      181 gaggaacata agttggggtt atttgtattc ccaatgtatc ataagcacgc caaatacctt
      241 ctccggttac gttatacacc tgaagcgtgt aagtaactcc gcatacggta tcatgattca
      301 tgcttaccgt aggtgtaggg ccttgaacaa aagtaacatt aacctgatcc tgactccagc
      361 acgagccatt ttgttctgtc atagtaaact ggtatgttcc atacgtctga atggttgccg
      421 tggttgcagg agcgctcaca ggattaaagc ttaccccatt tcccgaccat gtaattgtgc
      481 tgccgggaat tgattggttc ccgtttaaat caactgcagt tccgcaaaca gttacgtcag
      541 cacctgcata aggattcgga gtacatctga aatttacagt aacatcgtcc gaggcagtac
      601 actgaccatt aacaatattc catgaaaaag tacatgtctg gcaaaggttt gtcattgtga
      661 gcatattggg tgtacttacc gaagatccgc caacagggaa gaaatcacaa tcactcgacc
      721 agtaaccaat cattcctgat ggaacaggat tgccgttcaa gcgaatcaga agcccgcagg
      781 tatcgcgatc ggtaccggca taaggggtcg gattctgtag ccacattacc ggagttccca
      841 cagcaatgct gtagcaccct gtaagctcag gatgcccgtc tccattgtta tcgtatcctg
      901 caattgcaga aatgtagtat gtcgtgttgt acgagaaacc cggaatctgg ttctgacaga
      961 agttagttgt tgcagaatag gccaacggaa cattattccc attatggatc ataaattcaa
     1021 aaagtgaatt tcccaacata atctgatcac cattatgtcc aacgtaatca cccgtgcagg
     1081 ttccctgaca caaaatttgc aactgaaggg ttgtcattgt tccggcactc gggcagttac
     1141 aatcgcggaa acctgaaatg ggaatgtttg tacagccatt aacatcagtt atagtgtaag
     1201 cataattggt tgcagatgtg tgggtctctt caaaatgccc ggaagtggta acaccattta
     1261 cgaggtaatt tccggtaaaa ggtccgtcaa caccttccat atcccattca gttacatata
     1321 cgggggttgt cccatagatg cattcctgat cgttgaaatt cacaatgtgt aattcatcaa
     1381 aaacggtgaa agtagttgaa ctggctcctg ctccggcaca agggcccgtt atggtgttgg
     1441 ttatagtgta gtttccgggt ccggcgtttt caggggtgaa gataccactt gcagggtcga
     1501 aagatcctgc cgggcacgaa gggcaagtca atgtcccgaa atcaactgct tgaacctgaa
     1561 caggctcgtg tgatgcacac atatcatcga ttggcgttac agttgggtca aatgtttcat
     1621 tgaaagtaat attaacagta tccgagcttg aacaggcctg attgccctga taatattcag
     1681 tccatctgaa ctggtatgtg ccataaatat tattgataca aacctgagtg ttgtaaagtg
     1741 tattgttggt aaaagtagca gttccgggac cgctaacttg tgtccaaata ccattgtttc
     1801 cggctacagg ctggtttgca cttaaattgt aacaaacatc gcaaattgca gcagctgttc
     1861 cggctgatgg gtttggcatc tggcagcagt tggggtcggt aattggcctc acggtaacgg
     1921 taatcgtagt gtcgtaggta cagccaaaat tgtcggttgc atagaatgtg tagggaacgt
     1981 ttgtacctgc aagtaaaggc tgtgctgacc caattgagcc gatattatca atcatgttgt
     2041 ttccatccca atgtgcgtcg ggagaattgt tcccaagtac atacgaattc tcgaaacccc
     2101 ataaactttg gggtaatata ttatcgttga agttaatatc gcaagtgaaa ataataccat
     2161 cgtcttgtcc ccagttatcg ataaacttaa tacaccactc cccgttgatt gggcaaccaa
     2221 taaggctgct aaaagagctg cctgatggca aaggcagata agtgccggct ggaacagaac
     2281 tgcctgatga acaaactgag gtaattaatt gagtgccgct catcgaccag caataatccc
     2341 aacctgttcc cggttggcag ttatcggcat gatttggaac cccaaagtag gcaccattgc
     2401 aattatagta ttcatgcaaa ctggcctcct gcccatttgg acaaacaacc caaatataaa
     2461 ggtctccgat aaaggagtga tccatattta tacaagctga ttcaaggtct gttgccgact
     2521 gtatggtttg ccctggtgca aatgaattta cctgaaaaca cacttcctga acgtctccca
     2581 cattatcgtc gatacactcc tgaactatct gggtgtcggt aaccgtaatc tcccaggggg
     2641 ttgtatgaac aaccccgttt agattgactg tctgccccgg gcatattgga ttgggtgata
     2701 gagatgttcc gttgaagtgc ggcggaatgg aaacgcgtat ccgcaaattg gcctctccca
     2761 tattccagca gccttccaca tcggttgatc ttaagcgtag cgtatatcca cctggatttg
     2821 taaatgtata tgatagtgtt gttaaaccaa cgccgtcgtc atcaataatt gaaagtccgg
     2881 ttccatcaag gagttcccaa tagtaatgtg tgtttgctgt ggtttgatta tacccttctg
     2941 aaccattgtt cgggaaagtg ctaagagcag taaaggtgac cccatctccc ggacaaatgt
     3001 caagatgcat ggaatcggta tctgttatag ggggattaga agtaatttca gttgtgtagc
     3061 cctgacataa agttgcacag gtaattgtag cagcaaaacc ggaagcaaca ccgctggcat
     3121 ttgatgtaaa aacaatcgta atacagtcca caagtgaatt aattgtttga ccgttaaggg
     3181 tagttccact tccgcttgca agaaggttgt ttaagccacc aacaccgttg taaatcgaaa
     3241 tattgtcgtt tgctgcaagg ttgaaggcag taaaatgcaa cgataatgtt gagccgttgt
     3301 tactacaaag tacaaaggta atattctcgt tgttaccata gttgccgctg gctccaccag
     3361 aatcgtaaag tgtaccgctg caggtattta ctgtggtatt gcttaaatta taagtctgag
     3421 caaaagtgat attggctgca agaagcaata atcctgcgat tatggtaatt tgtatctttt
     3481 tcatgactta ttggtttttt gattgattat acatagttac catctctttt gaagagtaga
     3541 aacctaagac tataccggta tcgcctactc tgtaaaaaac tctcctgtcg tattgttggt
     3601 caacccaata caaagcaatg ttaacactcg aaaagtcgat tgaagtaact ttgttttcgg
     3661 attggaatcc tgtttctggg tcaacatagt acaaatatgg gctatctttt actttatcga
     3721 aaatagcatc ttccacaata aaccacgaat ggtcaaggtt gaagttaagt acgtccagtt
     3781 gaaccggtgt gtttattttc atttgttgca gattatcctc gctgtatatt gccttcagtc
     3841 gtggatcggg ctcaatagag ctctgggcaa cagcaacgcc cgaaattagc aggattagaa
     3901 aaataccagc cagtttgatt tttttcataa tatttgattt agttttttat gcgatttaca
     3961 ctgattagac attcagagat taaaaatagt tgtccggata ttatattaaa gcagtttttt
     4021 ttgcaattcc gcagtatttt tattgtgcgg cataaaaata cataaaattc ttattcaaat
     4081 gaaaaaaaat tgcattacgt gcgtgtaagc gttgtatgct atttttctct catgaggtgc
     4141 attggtccct ggaactcgta ttccacatcg tcga
//
LOCUS       DAOOQP010000179         4104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_25863_length_4104_cov_4.322302, whole genome shotgun sequence.
ACCESSION   DAOOQP010000179 DAOOQP010000000
VERSION     DAOOQP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25863_length_4104_cov_4.322302"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..>4104
                     /locus_tag="PLK75_12385"
     CDS             199..>4104
                     /locus_tag="PLK75_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOY40087.1"
                     /translation="MRKLFYVLTTLFVIVCMSQPAFSVPAKPGLIEFTQPDGSTLKIY
                     LHGDEFVKWAETLDGYTLMFNSEGYYEYATRDLHGEMIPSGIVARDIEIRDAAAVAYL
                     INTPKGLFYNQEQMSYLQQIRNMYQAELKSTKAFPTTGDRKLICILIGYTDRPFTKTQ
                     ADFNALFNQINYTIGSATGSVKDYYLENSYGQFDLTVDVAGPYTASNTLAYYGTNDGS
                     NNDMYPRQLVAEAVNLADPDVDYSLYDNDDNGTVDGVYVIYAGYGEEAGAPSNTIWAH
                     AWSITPVNKDGVSISKYSCSSELRGTSGTNITRIGVICHEFGHVLGAPDYYDTDYSTN
                     GQYDGTGEWDMMANGSWNNNGATPAHHNAYTKIYIYHWAEAITLTTPQNIAMLNAEQN
                     ASSFYRYNTQTSNEYFLLENRQKIGFDYACPGVGMIIYHVDGSYIASHTSANNINAAS
                     HQGMFPMAANASTANGVMTGSGAVSTSGCPWPGTSAKTSFTDATTPNSKSWAGVNTAL
                     PITNITVNGNIIQFCFISCPPACTAPTTQVSGFSTSNIGDNQIQVNWNRGNGTGVIVL
                     ARQGSAVDSNPINSVSYTADPVYGTGMEVGNGNYVVYTGAGTNVTVTGLTPGTLYHFA
                     VYEYFIATYCYMLPGITGNAITTGVVPNFCDTLSQFCCTASLYTVTNSGAYAGYLSGT
                     NSYGFEGFAEYFEDHEPYNQVWGMRVYLGEVNNVTNPNVTFAMWDNANGVPTNQLATK
                     IIPLSTIQTAFTNRGYIDIDFDDTISAPANGFYLGLLEPGSAASGDTIAIVTNAQDEG
                     LNSGFTIYGSWTPYNDLFGGTFQHAIYAFACFDNSIPPVAGFVGTPRRVALGETVQFN
                     DASAGTDPTSWTWTFEGGSPASSADANPQVTYETLGFFDVSLTVSNTNGSNSITQTDY
                     IEVFDPNSTTAFSLDFEACTDFQVDNFDPWTTLDVDGKTTYGSQNFDFPNESYTGSFI
                     AFNSANTTPAATGWEAHGGNLCGICMAATTPPNNDWLMSTQIDLGENSSFSFWAKSIT
                     DQYGLERFIVYVSTTGNATTDFVKISEGTYVQAPVTWTQYTYNLSAFDNQSVYVAIRC
                     VSNDAYAFMIDDIEIHSVYVAPTCNFIADNTNVLEGSTINFTDLSTQMPTSWQWSFPG
                     GTPATSNEQNPSVVYNVPGTYDVSLTVTNSAGTDSETKSAYIMVVETPEVIVLWDFPN
                     SPDNNVADAGITTNLTKTIVPFGGVSDILYSAAGVTTRSIDGETWASGSGTKGWSVNF
                     STLGYGTLKLSFAQMSNNAASPRNFKIQYSLNGTSWTDLG"
BASE COUNT         1179 a          886 c          881 g         1158 t
ORIGIN      
        1 ataatattca gaaatttaaa ttacatacca gcaattgaca attaagaatt acttttgata
       61 ggtgatttgt gtgtcagtaa actatactga aatttttgtg tttattattg aatttgttgt
      121 gctgtaagct ttgtaatatt aaggtatgag tctttgtatc tctgatattt tcatattttc
      181 aaaaataatc ttcgttttat gagaaaactg ttttacgtat taactacgtt atttgtaatt
      241 gtgtgtatga gccagcctgc attctcggtt cctgcaaagc ctggtttgat tgaatttacc
      301 caaccggatg gatccacatt gaagatttat cttcatggtg atgaatttgt aaagtgggca
      361 gaaaccctcg atggctatac cctgatgttt aatagcgaag gttactatga atatgccacc
      421 cgcgacctgc atggcgaaat gattcccagc ggaattgtcg cccgcgatat tgaaataaga
      481 gacgcagcag cagttgctta tcttatcaac acaccgaaag gcttgttcta caatcaggag
      541 cagatgtcgt atctgcagca gatcaggaac atgtatcagg cagaattaaa aagcacaaag
      601 gcatttccaa caacgggcga tagaaagcta atatgtattc ttattggcta taccgaccgc
      661 ccattcacca aaactcaggc tgatttcaat gcattgttta atcagattaa ctatacaatt
      721 ggcagtgcta caggaagtgt taaagactac tatctggaga attcctatgg tcagttcgac
      781 ctgacagtag atgttgcagg tccctacaca gcttcaaata cactagcata ttatggaacc
      841 aatgacggta gcaataacga catgtatccc agacaacttg ttgcagaagc cgtaaacctt
      901 gctgaccctg acgtggacta ttctttgtac gataatgatg ataacggaac tgttgatgga
      961 gtatatgtga tctatgccgg atatggcgaa gaagccggag ctccgtcaaa tactatctgg
     1021 gctcatgcct ggtcgattac tcctgtaaat aaagacggtg taagcatttc gaaatattca
     1081 tgttcatccg agttaagagg aacaagcggt accaacatta cccgtatcgg ggtgatatgc
     1141 catgaatttg ggcatgttct gggtgcaccc gactactacg ataccgatta ctccaccaat
     1201 ggtcaatatg atggtaccgg tgaatgggat atgatggcaa acggttcatg gaacaacaac
     1261 ggtgcaacac ccgcccacca caacgcctat accaaaatat atatttatca ttgggcagag
     1321 gctataacac ttacaacccc tcaaaacatt gcgatgttga atgctgaaca aaatgcatca
     1381 agtttttaca ggtacaatac ccaaacgtca aatgagtatt ttttacttga aaaccggcag
     1441 aaaattggat tcgactacgc ttgccccggt gttggtatga ttatttatca tgttgacggg
     1501 agttatattg catcgcacac ctctgcaaac aacattaatg cagcaagtca tcagggcatg
     1561 tttcctatgg ctgcaaatgc atcgactgca aacggtgtaa tgacggggtc gggtgctgta
     1621 agtacatcgg gatgcccatg gcctggaact agcgcaaaaa caagcttcac cgatgcaaca
     1681 actcctaact ccaaatcatg ggccggagta aatacagctc tgcctattac caacattaca
     1741 gtcaatggca atattattca attttgtttt atcagctgcc cgccggcctg tacggctcca
     1801 actacccagg tgtcgggatt ttctacttca aatatcggag ataaccagat tcaggttaac
     1861 tggaacagag gcaatggaac cggtgtaatt gttctggcac ggcaaggctc tgcggttgac
     1921 tcgaatccga taaactcagt gagttataca gcggaccctg tatatggaac cggaatggaa
     1981 gtcgggaacg gcaattatgt tgtttacacc ggagcaggaa ctaatgtaac tgttacaggt
     2041 ttaactccag ggacattata tcattttgct gtttatgaat atttcattgc aacttactgc
     2101 tatatgctac ccggtataac agggaatgca ataacaaccg gtgttgtccc caacttttgt
     2161 gatacactga gtcagttttg ctgtactgca agtttgtaca cagttaccaa ttccggggca
     2221 tatgccggct atttgagtgg caccaacagc tacgggttcg aaggttttgc cgaatacttc
     2281 gaagaccacg aaccgtataa tcaggtttgg ggtatgcgtg tttaccttgg tgaggtcaat
     2341 aatgtaacaa acccaaatgt aacttttgcc atgtgggata atgcgaatgg tgttccaacc
     2401 aatcagctgg ctacaaaaat aattcccttg tcaacaattc aaacagcgtt tacaaatcgg
     2461 ggttatattg atattgattt tgatgatacc attagcgctc cggccaatgg cttttacctc
     2521 ggcctgctgg agccagggag tgctgcttct ggcgacacca tagccattgt taccaatgct
     2581 caggacgaag ggcttaattc cgggtttacg atttatggca gttggactcc atataacgat
     2641 ttattcggag gtacgttcca acatgcaatt tatgcatttg catgtttcga caacagcatt
     2701 ccacctgttg ctggatttgt aggtacaccc cgacgcgtag ccctgggaga aacggttcag
     2761 tttaacgatg cttctgccgg tactgatcct acttcatgga catggacatt tgaaggaggc
     2821 tcccctgctt caagtgctga tgccaatcca caagtaacat acgagacttt aggttttttt
     2881 gatgtatcgt taacagtttc taacactaac ggaagcaatt ccataactca gacagattat
     2941 attgaggttt tcgaccccaa cagtacaaca gccttttctc tcgattttga agcctgtacc
     3001 gattttcagg ttgacaattt cgacccctgg acaactttag atgttgacgg aaaaacaact
     3061 tacggatcgc aaaattttga ttttccgaac gaaagctata ccggttcatt tatcgcattc
     3121 aactcggcca acaccactcc ggcagccacc ggctgggaag cccatggagg caatctgtgt
     3181 ggtatatgca tggcagctac tacaccgccc aataacgact ggctgatgtc gactcaaatt
     3241 gacctgggtg agaattcttc gttttctttc tgggctaaat cgataaccga ccagtacggt
     3301 cttgaaaggt ttattgttta tgtatcaact accggtaatg ccacaaccga ttttgtaaaa
     3361 atatctgaag gcacttatgt acaggcacct gtaacatgga cccaatatac ctataaccta
     3421 tcggcattcg acaaccaatc ggtatatgtt gctatacgtt gcgtttcgaa cgacgcctat
     3481 gcctttatga ttgatgacat tgaaatacac agtgtatatg tggctccaac gtgtaatttt
     3541 attgccgata acacaaatgt tttggagggt tcaaccatca atttcaccga tttatcaacc
     3601 cagatgccta caagttggca gtggtcattc cccggtggaa cacctgcgac cagtaatgaa
     3661 caaaacccaa gcgttgttta caatgtacca ggcacttatg atgttagcct gactgtaaca
     3721 aactcagcag gaaccgacag tgaaacaaaa tcagcctata ttatggtggt tgagacccct
     3781 gaagtaattg tactgtggga ttttccaaat agtccggaca acaatgttgc agatgctgga
     3841 attactacca atctaacaaa aactattgtt ccatttggtg gtgttagcga cattctatac
     3901 tcagctgcag gtgttaccac ccgttcaatc gacggcgaaa catgggcttc cggttcagga
     3961 accaagggct ggtctgtgaa tttctcaaca ctgggttatg gcaccttaaa actttcgttt
     4021 gcacagatgt cgaacaatgc tgcgtctccc cgtaacttta aaattcaata cagcctgaat
     4081 ggtacaagct ggacagattt ggga
//
LOCUS       DAOOQP010000180         4104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_25864_length_4104_cov_4.178563, whole genome shotgun sequence.
ACCESSION   DAOOQP010000180 DAOOQP010000000
VERSION     DAOOQP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25864_length_4104_cov_4.178563"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLK75_12390"
     CDS             <1..758
                     /locus_tag="PLK75_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40088.1"
                     /translation="LWAFNFGNSQWESYPAYSGDGRHGHIAARKDSEMYIIGGYEGFS
                     AVNTIICYHTGSGTWSTLSKKGSFPEPFAYAGHSQIDNQVFIFGGATNSDVTGNAYLW
                     DLNEMNFAPLAVGPVRSDPGVTHVPGPSQDKDAQYQAFMLFGGSNMGLIEDETWYYYS
                     DIEIQSNISTCLGNPHIQVSQSGHQIFISSGTDILSGAEYQINDLQGHIFVKATITGQ
                     STCVDLPYACNGLFILTIKKNKIPVFITKLAVY"
     gene            complement(770..3358)
                     /gene="clpB"
                     /locus_tag="PLK75_12395"
     CDS             complement(770..3358)
                     /gene="clpB"
                     /locus_tag="PLK75_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOY40089.1"
                     /translation="MNFKDFTIKAQETVERAVTNAQQSQHQAIEPGHILKASFNTSEH
                     LTEHIWGLLGLDMQKFENILDSIVASYPKVQGGEVYMSRDTINAINKSRSFLKEFGDS
                     FISVELIILGIFHCGDTVSKMMRDMGIRDSDLRKAIQEMRKGNHVKTENSEDTYNALM
                     KYAINLNKRAIEGKLDPVIGRDEEIRRILQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIVAGDVPENLKTKQIFSLDMGSLIAGAKYKGEFEERLKAVVNEVIGSDGEIILFID
                     EIHTLVGAGKSEGAMDAANILKPALAKGDLRAVGATTLSEYQKYFEKDKALERRFQIV
                     MVEEPDEESAIAILRGLKERYENHHKVRILDEAIVSAVQLSARYITERFLPDKAIDLI
                     DEAASKLRLEMNSMPEEIDDLQRRIRQFEIEREAIKRENDKERILAISRHIENLSEEK
                     NILLAKWEMERTLIAKIQQRKQDIENYRTEAINAERVGDYGKVAEIRYGKINSAEMEI
                     EATQKELHKVQADSALIDEVVGADDIADIISKATGIPVARMVQSEREKLMKLEEYLHK
                     RVVGQDEAITAVSDAVRRSRAGLQDKNRPIGSFIFLGTTGVGKTELAKALAEFLFDDE
                     RFITRIDMSEYQEKHTVSRLIGAPPGYIGYEEGGQLTEAVRRKPYSVILLDEIEKAHP
                     DVFNILLQVLEDGRLTDNKGRTANFKNTIIIMTSNSGSHIISEKMQKLTAKNRDAIFE
                     ETKNDVLQLLRKQMRPEFLNRIDEIVMFTPLSRVEIQDIVRLQLQMVQVRLKAQGITF
                     DATEKAIAAIADAGFDEQYGARPLKRVIQKELLNYISKMIISGEIDIEKTIIADTDSK
                     GLKVYN"
     gene            complement(3473..4045)
                     /locus_tag="PLK75_12400"
     CDS             complement(3473..4045)
                     /locus_tag="PLK75_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOY40090.1"
                     /translation="MRKVLVIDNHDSFVHNIIGLLRQFDDVFVERMYNDNIVFSHLNN
                     FDTLLLSPGPGLPKQSNSLMKCIETCVNTHKILGVCLGHQAICEFYGAKLIQLPSIKH
                     GHKASITKTDSNDKLLKKLPRVFEAALYNSWGVSGIDFPEELLVSSVDESGNIMSVYH
                     KTLCIHGVQFHPESIVSNCGTTIVQSLLEL"
BASE COUNT         1081 a          908 c          760 g         1355 t
ORIGIN      
        1 atttgtgggc tttcaatttc ggtaattcac agtgggaaag ctatccggct tattcaggcg
       61 atggacgaca cggacatatt gcagcacgta aagactcgga gatgtatatt attggcggtt
      121 atgaaggatt tagcgcagta aacacaatta tatgctacca tacaggttca ggaacatggt
      181 ccaccttaag taaaaagggt tctttccctg aaccgtttgc gtatgccggt cattcacaaa
      241 tcgacaatca ggtttttata tttggcggag ctacaaattc cgatgttacc ggaaatgctt
      301 atttatggga tcttaacgaa atgaacttcg cacctcttgc tgtaggccct gtgcgctccg
      361 atccgggtgt aacccatgtc cctggcccgt cgcaggacaa agacgcccag tatcaggcat
      421 ttatgctttt cggaggctca aacatggggc ttatcgaaga cgaaacatgg tattactatt
      481 ccgatattga gatacaatcc aatatttcga catgtcttgg taacccacac attcaggtgt
      541 cacaatcagg gcatcagatt ttcatttctt caggcaccga catactttcg ggggctgaat
      601 atcaaataaa cgatttgcaa ggacacatct ttgtgaaagc aaccattacc ggtcaaagca
      661 cttgtgttga cttaccctat gcctgcaatg gtctgtttat tctaaccatc aagaaaaaca
      721 aaatacctgt tttcattacc aaactggctg tatattagca ataatttggt taattataca
      781 ctttcaagcc cttactatcg gtatcggcta ttatcgtttt ctcaatatca atttcgcccg
      841 agattatcat tttcgagatg tagttgagta attctttctg tatcacccgc ttcaagggtc
      901 gtgctccata ttgttcatca aatcctgcat cagcgattgc agctatagct ttctcagtag
      961 catcaaatgt aatgccttga gcttttagtc tgacctgaac catttgtaat tgtagtctta
     1021 caatatcctg tatctctact ctgctaagcg gtgtaaacat tacaatttcg tcgatacggt
     1081 ttagaaattc cggacgcatt tgtttgcgta gcaactgcaa aacatcgttt ttagtttctt
     1141 caaatattgc atcacgattt ttagcagtta acttctgcat tttttcgctg attatatggg
     1201 agccgctatt actggtcata atgataatgg tgtttttgaa gtttgcagtc cgtcccttat
     1261 tatcagtgag tcgtccgtct tcgagtacct gaagcagaat attaaaaacg tcggggtgtg
     1321 ctttctcgat ttcgtcgaga agaatcactg aatacggctt tcttctcact gcttcggtaa
     1381 gttgccctcc ttcttcgtaa ccaatatatc cgggaggtgc tccgatcaaa cgcgataccg
     1441 tatgtttttc ctgatactcg ctcatatcga tgcgtgttat aaaccgttcg tcgtcgaata
     1501 aaaattcggc aagtgcttta gcaagttcag ttttaccaac accagtagtt ccaaggaaga
     1561 taaacgagcc aatgggtctg tttttgtcct gcagccctgc tctgctgcgt cgcactgcgt
     1621 cgctgacagc agtaatagct tcgtcctgcc ctactactct tttgtgcaga tattcttcaa
     1681 gtttcataag tttttcgcgt tccgactgca ccatccgggc aactggaatg cctgttgctt
     1741 tactgattat atcggcaata tcgtcagcac ccacaacttc atcgatcaga gcactatcag
     1801 cctgaacttt gtgtaactct ttctgcgttg cctcaatttc catttcagcg gaattaatct
     1861 taccatatcg tatttcggcc accttaccgt aatcgccaac acgttcagca tttatagctt
     1921 ctgtacgata attctcgata tcttgtttac gttgttgaat tttggctatt aaagtgcgtt
     1981 ccatttccca ttttgcaaga agaatgtttt tctcttccga taaattctca atatgccggc
     2041 tgattgcaag aatacgctct ttatcatttt cgcgcttaat agcttcacgt tctatttcga
     2101 attgacgaat cctgcgttga aggtcatcga tttcttcagg cattgaattc atttcgagac
     2161 gaagttttga tgcagcttca tcaataaggt caatcgcttt atcgggtaaa aacctctcgg
     2221 taatatatcg tgctgaaagt tgtactgcag acactattgc ttcatcaaga attctgacct
     2281 tatgatgatt ttcgtagcgt tctttcaagc cacgtaaaat ggctatggcg ctttcttcat
     2341 cgggttcttc taccatcaca atctgaaaac gtctttctag agctttatcc ttttcgaagt
     2401 atttctgata ttccgataag gtggttgcgc ctactgctct taagtcgcct ttagcaagag
     2461 ctggttttag tatgttggcc gcatccatgg ctccttcgct tttacccgca cccaccagtg
     2521 tgtgtatttc gtcgataaac aatattatct ctccatcact cccgataacc tcgtttacca
     2581 ctgcttttaa tctttcttcg aattcgcctt tatatttagc ccctgcaatt aaagagccca
     2641 tgtcgagaga gaaaatttgt tttgttttca ggttttccgg aacatctcct gcaacaatac
     2701 gatgtgcaag cccttcagca atggctgttt tcccaacccc cggctcacca atgaggattg
     2761 gattgttttt agttcgtctc gaaagaatct gaaggattct cctgatttct tcgtcacgtc
     2821 cgattacggg atcgagctta ccttcaattg cccgcttatt cagattgata gcgtatttca
     2881 tcaatgcatt atacgtgtct tcagagtttt ctgtttttac atgattgccc ttacgcattt
     2941 cctgtattgc ctttctcagg tcactatcac ggatacccat gtcacgcatc attttcgaaa
     3001 cggtatcgcc acaatgaaaa atacctagga taatcaattc aacagaaatg aaactatcgc
     3061 caaattcttt aagaaagcta cggctcttat ttatcgcatt tattgtatct ctcgacatat
     3121 atacctctcc tccctgcact ttcgggtatg atgcaacaat gctgtctagt atgttttcga
     3181 acttttgcat atcaagtcca agcaaacccc aaatgtgttc tgtaaggtgc tcacttgtat
     3241 taaaagatgc tttgagtata tgtccgggct ctatggcctg atgctggctt tgttgagcat
     3301 ttgtcacagc cctttcaaca gtttcttgcg ctttaattgt aaaatccttg aaattcattg
     3361 ctttttattt ttagttcaaa gttgacagtc aaaagcattg ccaatttata taaaactgtc
     3421 ataatgacat tgtttgctta tacaaaacga cattatgaca gtattagtaa agctataatt
     3481 ccaaaagtga ttgtactatc gttgttccac agttgctaac aatcgattcg ggatggaatt
     3541 gtacgccgtg aatacaaagt gttttatggt ataccgacat gatatttcca gattcatcaa
     3601 cagagcttac caaaagctct tccggaaaat caatcccact aacaccccat gaattataaa
     3661 gtgctgcttc gaaaactcgc ggcagttttt taagcagttt atcgtttgag tcagtttttg
     3721 ttatcgatgc tttatgccca tgttttatac ttggcaactg aataagttta gccccataaa
     3781 attcgcaaat agcctgatgt ccgaggcata cgccaagtat tttgtgggta ttcacgcagg
     3841 tttctatgca tttcatcagg ctattacttt gcttcggcaa gcccggtccg ggagaaagca
     3901 ataatgtgtc gaaattgttc aagtggctga agacaatatt atcattatac attctttcaa
     3961 caaaaacatc gtcaaactgt ctcaggaggc caatgatgtt atgcacaaac gaatcgtgat
     4021 tatctataac caaaactttt cgcatactca gaaactgttt tgaattatac taataatatg
     4081 ataattaaca acaaaaagtt tata
//
LOCUS       DAOOQP010000181         4041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_26448_length_4041_cov_4.553939, whole genome shotgun sequence.
ACCESSION   DAOOQP010000181 DAOOQP010000000
VERSION     DAOOQP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26448_length_4041_cov_4.553939"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLK75_12405"
     CDS             complement(<1..762)
                     /locus_tag="PLK75_12405"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOY40091.1"
                     /translation="MLFNNTYYRAVWMEADNVFMIDQNVLPFRFSVICCKKYTDTCNA
                     IVNMTTRGAGSIGAAAGFAMAQACLQAKYENTADFLLKSRQQIESTRPTARNLFYATE
                     KVFNAALISVEQALVMAQKVADECADQGRRIGVNGNALLAAGIRVLTHCNAGWLALVD
                     YGSALAPIYEAHSCLKKPFVYVDETRPRGQGARLTAWELFHQGIDHKIIPDNAAAHFM
                     SQGAIDICIVGADRIAHNGDTANKIGTLEKALAAKY"
     gene            complement(838..1572)
                     /locus_tag="PLK75_12410"
     CDS             complement(838..1572)
                     /locus_tag="PLK75_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY40092.1"
                     /translation="MTKPLLHTVDLSVGYSAKKPVLSNVTVTVLPSELVMVAGRNGSG
                     KSTLLTTILGLLPALSGDILIKGRSIRKYSHAGRSRTLSYVPSKSNVINNFTIQQIIE
                     TGRAPYTGWNNRFSEADRFHVDEATANLGLSNILHRRLNEVSDGERQKAMIARAIAQD
                     APIIILDEPTAFLDYPTRIELIQILKMLTREMKKAIVLSSHDLDILLPVVDSIWFCSD
                     NGIVAEKADSMMQNNGFKMFFNKPSL"
     gene            complement(1575..2603)
                     /locus_tag="PLK75_12415"
     CDS             complement(1575..2603)
                     /locus_tag="PLK75_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOY40093.1"
                     /translation="MQKNQGNTVRFGILLVVTLVLLILDFQIGTTHISLSDLISFLFN
                     QRELDIQKISLIKEFRLTRVITAVLAGGGLSLAGLLMQSIFRNPLAGPYVLGVSSGAG
                     LGVAILVMGASIAGVNVFFLNSGIFAVFAAMAGAALVMLIVISASLRMRDNVTVLIIG
                     ILLGAVITALISVLQFFADSLSVKSFVIWGMGSLSALSFNNMVLLAVIILLSFIATFS
                     ICKPLNLFLLGEENASVSGVNTRKLRVVVFAITCVLAGTITAFCGPIGFIGIAVPHMS
                     RWMFKTASHFVLVPAVFLTGATVMLLSDMLSYSFGNAGVLPINAVTAVTGVPVIIYVV
                     IRNQKSYF"
     gene            complement(2615..3745)
                     /locus_tag="PLK75_12420"
     CDS             complement(2615..3745)
                     /locus_tag="PLK75_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY40094.1"
                     /translation="MKYLWCKPAVLLFWGTIVMLTSCTDSGENRKADDKPIKNKYARG
                     FEIIQQSQGYTLRVFDPAQNSKNNSYSYRLTRNSAKKNEIHVPVKRIICCSTSHVAFL
                     SALNEADKLTGISTGKLVFDTLVSGRFYRGEILDIGYETTFDMEVIVSLKPDVIFAYG
                     VDYNSLGSFQKIEAMGIPVVWIGEYLEPDILGRTEWLLFFSCFFDKLNYAHVQFDSIA
                     DRYNKLVTTATQHKGGRPLVITGLPWRGTWFVPGGKSYFSSLIRDAGGEYIFSKDTHT
                     ESIPVPLEDVFYQAQNVSVWLNPNNCVNKKEILVTDSRFDNFAPLHQAIICNNNKRMN
                     HNGGNDFWESGIIHPDIVLNDLIHIFHDTIINEDSLFYYRKL"
     gene            complement(3778..>4041)
                     /locus_tag="PLK75_12425"
     CDS             complement(3778..>4041)
                     /locus_tag="PLK75_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40095.1"
                     /translation="ENMINVDITVYPNPASDMFTLEFGDEFMGKDAVVSVRNILGSSV
                     IDISALNISGEQKIDISALSEGIYLVSINIEGKTYTKRISVVK"
BASE COUNT         1246 a          926 c          710 g         1159 t
ORIGIN      
        1 atattttgca gccaaggctt tttcaagcgt tccaatttta tttgctgtat ctccgttatg
       61 tgcaatacgg tcggcaccaa caatacatat atctatagca ccctggctca taaaatgtgc
      121 ggctgcatta tcgggaataa ttttatgatc gataccctga tggaacagtt cccatgcagt
      181 caaccgggct ccctgtccgc ggggtcgggt ttcatcgaca taaacaaatg gtttctttaa
      241 gcaagagtgc gcctcatata ttggtgccag agcactccca taatcaacaa gcgctaacca
      301 accggcattg caatgggtaa gaactcttat acctgctgct agcagtgcat tcccgttaac
      361 gccaatgcgc ctgccttggt cagcacattc atcagcaact ttctgcgcca tcactaacgc
      421 ctgttcaacg gaaatcaaag ctgcattgaa tactttttcg gtagcataaa acaaattccg
      481 agctgttggg cgggttgatt caatctgctg cctggatttt aataaaaaat cagcagtatt
      541 ttcgtattta gcctgcaggc atgcctgagc cattgcaaaa ccggcagctg ctccaattga
      601 cccggctcct cttgtagtca tgtttacaat tgcattgcat gtatcggtat attttttaca
      661 acaaatcacc gaaaaccgaa aaggcagcac attctgatca atcataaaca cgttatcagc
      721 ttccatccac actgcccgat aatacgtatt attaaacaac atgtttcagt tacaatttaa
      781 ttcatttttc acgatgcgat atacaattaa aaacactagc ctgataaatt aatcttttca
      841 taatgaaggt ttattaaaaa acattttaaa gccattattt tgcatcatac tatctgcctt
      901 ttcggcaaca attccgttat cgctgcaaaa ccagatacta tcaactaccg gtaaaagtat
      961 atcaaggtcg tgcgacgaca aaacaatagc ctttttcatt tctcttgtaa gcatttttaa
     1021 aatctgaata agttcaattc ttgtagggta gtcgaggaat gcagttggtt catctaaaat
     1081 aataatcgga gcatcctgag ctattgcccg ggcaatcatt gctttttgtc tctccccgtc
     1141 ggaaacttca ttcaaccttc tatgtaaaat attgctcaac cccagattgg ccgttgcttc
     1201 atcaacatga aacctgtcgg cttcagaaaa tcgattattc catcctgtgt aaggagcccg
     1261 tcctgtttcg ataatttgct ggattgtgaa gttgtttatg acatttgatt ttgatggcac
     1321 ataactcaag gtccgcgacc ttcctgcatg agaatattta cggattgacc tgccctttat
     1381 taaaatatct cccgataaag caggcagcag tcccagtatt gttgtgagca atgtgctttt
     1441 ccctgaacca tttcgtcctg ctaccataac caattctgaa ggtagcaccg ttacggtaac
     1501 attggataac actggtttct ttgcagaata tccgacacta aggtcaacag tatgcaataa
     1561 gggttttgtc ataatcagaa atatgatttc tggtttctta tcacaacata aattatcacc
     1621 ggaacacccg tgactgcagt tacagcatta ataggtaaca ctccggcatt accgaaggaa
     1681 tacgacaaca tatcgctcag cagcattacc gtagctccgg taagaaatac agcgggaaca
     1741 agcacaaaat ggctggccgt tttaaacatc caccgactca tatgtggtac tgcaatacca
     1801 ataaaaccaa tgggtccgca aaaagcagtg attgtgccgg caagaacaca agtaatcgca
     1861 aataccacca ctctcaactt acgagtattc acgcccgaaa cactggcgtt ttcctcgccc
     1921 agtaagaaca ggttcagcgg tttgcaaatt gaaaacgtag caataaacga aaggagtatt
     1981 ataacagcaa gtaataccat gttgttgaac gacagagccg aaaggctccc cattccccat
     2041 attacaaatg attttacgct taaactatcc gcaaaaaact gtaatacact aatcagtgcg
     2101 gtaattactg ctccaagtaa aataccaata ataagtacag taacattatc gcgcattcga
     2161 agtgatgctg aaatcactat tagcattacc agagcagccc ctgccatagc agcaaaaaca
     2221 gcgaaaatac cgctattaag aaaaaacaca tttacacccg caatcgatgc tcccattaca
     2281 agaatagcaa ctcccaagcc tgcaccggag ctaacgccca aaacataggg tcccgccagt
     2341 gggttcctga aaatggactg catcaacaat cccgccagag aaagccctcc acctgccaaa
     2401 acagcagtaa tcacccttgt aagtcgaaac tcttttatca gggatatttt ctgaatatcg
     2461 agttcccttt gattaaataa aaacgaaatt agatcgctta aactaatatg tgtagtacca
     2521 atttgaaagt caagaatcaa aagaacaagt gtaacaacca gcagtattcc aaaccgaacc
     2581 gtatttccct gattcttttg catacacaaa tgaactaaag ttttcggtaa taaaaaagag
     2641 agtcttcatt tattatcgta tcatgaaaaa tgtgaatcag gtcatttagc actatgtcag
     2701 gatgtataat tccggactcc caaaagtcgt taccaccatt gtggttcatt cgtttattat
     2761 tgttgcatat tattgcctga tgcaaaggag caaaattatc aaaccgtgaa tcggttacca
     2821 gaatctcttt tttgtttaca cagttgtttg ggtttagcca gacactcaca ttctgcgcct
     2881 gataaaatac atcttcaaga ggtacaggaa tactttcggt atgggtatct ttcgaaaata
     2941 tgtattcgcc accggcatcc ctgattaaag atgaaaaata ggatttcccg cccggtacaa
     3001 accatgtccc cctccaaggc aaacctgtaa taaccaacgg tctgccacct ttatgctgtg
     3061 ttgcggttgt taccaacttg ttgtacctat ccgcaatact gtcaaattgt acgtgtgcat
     3121 aatttagttt atcgaaaaaa cacgaaaaaa acaatagcca ctccgtacgc cccaaaatat
     3181 caggttccag atactctcct atccatacta ccggaattcc catggcttca attttctgaa
     3241 aactacccaa agagttatag tcgacgccat atgcaaagat tacatccggc tttaaactta
     3301 ctataacctc catatcgaat gtagtttcgt agccgatatc aagaatttcg ccccgataga
     3361 accgtcctga taccagggta tcaaatacaa gtttaccggt tgatattcct gtcagtttat
     3421 cagcttcatt aagagctgat agaaatgcca catgtgatgt tgagcaacaa ataattcgtt
     3481 ttacagggac atgaatttcg tttttttttg ctgagtttct ggtaagtcgg taggaataac
     3541 tattgttttt gctgttctgt gcaggatcga aaaccctcag tgtgtatcct tggctctgct
     3601 gtataatttc gaaacctcgg gcatatttat tctttatagg cttatcatct gctttccggt
     3661 tctcccctga atcggtacag gaagttagca ttactatcgt accccaaaaa agaagaaccg
     3721 ctggtttaca ccataaatat ttcattggcc tatttaattg aaaaataaga ataccggtta
     3781 ttttacaacc gatattctct ttgtataggt tttaccctcg atatttatgc tcacaaggta
     3841 tataccctcc gagagagccg aaatatcgat tttttgctca ccgctgatat tcaaagcaga
     3901 aatgtcaatt acagaactgc cgagaatatt tctaacactc acaacagcgt ctttacccat
     3961 aaattcatca ccaaattcca gcgtaaacat atcgcttgca gggtttggat aaaccgtaat
     4021 atcaacgtta atcatattct c
//
LOCUS       DAOOQP010000182         4024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_26620_length_4024_cov_4.569665, whole genome shotgun sequence.
ACCESSION   DAOOQP010000182 DAOOQP010000000
VERSION     DAOOQP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26620_length_4024_cov_4.569665"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLK75_12430"
     CDS             complement(<1..235)
                     /locus_tag="PLK75_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40096.1"
                     /translation="MKKLLIFIVPFIVAAGLTSCINGHDPAIVSMAFHERIPGIDKDY
                     LNGAILQFDANGNIIVNEISRETRSYALSESGLL"
     gene            complement(232..1089)
                     /locus_tag="PLK75_12435"
     CDS             complement(232..1089)
                     /locus_tag="PLK75_12435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY40097.1"
                     /translation="MRNYLILLLLTLFLWGCKNPVNSFKKASSGLEYRFITENPEASA
                     AKPDELLVITLDVLTENGDLLFSSRKSDRKYLKKLHEPAHPGGSLEDALAMMHLGDSA
                     VFRVDAYNYYTYTEKLEKLPDKVKKGDKIILHIRLDEIISEGNYNDLLVEKYHKDEAT
                     ELSLLADYLKRTNVTVKPTPSGLYYIELKKGSGVKPSPGQKVKVHYTGCFIDGSMFET
                     SLTSRPIEFTLGNKEVIDGWDEGIALMQTGGKARLIIPSRIAYGALGKDEILPYSTLV
                     FDVELLETK"
     gene            complement(1096..1653)
                     /locus_tag="PLK75_12440"
     CDS             complement(1096..1653)
                     /locus_tag="PLK75_12440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY40098.1"
                     /translation="MKFSNKYLLAMLVFPFLAIVSCNTGNNNVAVDNENYSYQDVKSP
                     KDMKDMNRELIDMQDEMIKSYITRRKWDMQQTKTGLYYQIYHTSPSGVMPVVGKKAEF
                     AYKTYLLDGTLIYSSDSTGNREMLIGHNTEETGIDEGLRMLHVGDKARFILHPHLAYG
                     VPGDGHKVPYRAILVYDIELINVND"
     gene            complement(1640..2665)
                     /locus_tag="PLK75_12445"
     CDS             complement(1640..2665)
                     /locus_tag="PLK75_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOY40099.1"
                     /translation="MALIPKNSNSIVKFKEYVLRANHAVIIPHENPDGDAIGSALAMY
                     HLFISLEKKAEVVCPNDFPAFLKWMPGNDSIVIASKEKQKAKALISEADLIICVDFND
                     ITRMAVIQPMIKKAGADVILIDHHPGPVAFYNLLIHSTQSSSTAELIFEATEKAGFLK
                     YANRNFAECIYVGIMTDTGSFNFNSSNSNTFRIVSKILKFGVNKDAAFDNVYNNFSAS
                     RMRLLGYALNKALVVLPELKTAYIKLTLKDLQDYGYQDGDTEGFVNYPLSVAGIKVSA
                     IFIERKTFIKVSFRSKNKFAVNQFAAEYFKGGGHINAAGGEYHGSLDEAVEFFTNKIA
                     LYTNEIF"
     gene            complement(2905..>4024)
                     /locus_tag="PLK75_12450"
     CDS             complement(2905..>4024)
                     /locus_tag="PLK75_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY40100.1"
                     /translation="GCAPLTVTFTDASTNNPLTWLWNFDGENSANTANTSFTFETPGI
                     YTVSLTVENLDGADVETLQITVFNNPVIQLGDDIEDCIGNTVNLNAGEDFSSYLWHDG
                     YEDQIYEATQDGDYYVTVTDIHGCTASDEINVFFNPVPEINLGEDQTICDYETLTLDA
                     GDNLLSYEWSTGSVDQILVVDEGGTYSVIIMNEYHCSSSDEIVVTEIAAPELVLPETL
                     PGCVDELFSYTLTGTYDYIIWSDETTEQSFNATWAEPGEYEVWATVGVGECESTDTIM
                     VIVEVCQSINGIDNTIGISLYPNPASTETTLEITGYTGLIYCELTDIQGKLLWECHSY
                     ISDEFIQTIDMSGYVAGMYLLQIRHNDQVMNMKLIHDK"
BASE COUNT         1123 a          846 c          823 g         1232 t
ORIGIN      
        1 acagtaagcc cgattccgaa agggcatacg aacgtgtttc gcgactgatt tcgtttacaa
       61 taatatttcc attagcatcg aactgcaata tggcaccgtt tagataatct ttatcaatcc
      121 cgggaatccg ttcatgaaaa gccatagaaa caattgccgg atcatggccg tttatgcacg
      181 atgtgaggcc ggctgctact ataaaaggca ctatgaaaat aagtagcttt ttcatttagt
      241 ttcaaggagt tcaacatcga aaaccagtgt ggagtagggc aatatttcgt ccttacccaa
      301 tgcaccatag gcaatgcgtg atggaataat cagccgggct tttccgccgg tttgcataag
      361 cgctattccc tcgtcccagc catcgatcac ttctttgttg cccagcgtaa attcaatggg
      421 tctcgatgta agcgatgtct cgaacataga gccatcaata aaacagccag tgtagtggac
      481 tttaactttt tgcccggggc tgggtttgac cccgctgcct tttttcagct caatatagta
      541 cagccctgat ggtgttggtt tcacagttac gttagtgcgc ttcaggtaat cggcgagaag
      601 actgagttcc gtcgcctcgt ctttgtgata tttttctacc agcagatcgt tgtagttacc
      661 ctccgaaatt atttcatcca ggcgaatgtg cagtattatt ttatcgcctt tttttacctt
      721 atccggaagt ttttcaagtt tttcggtata ggtgtaataa ttgtaagcat cgaccctgaa
      781 aacagcgctg tcgcctaaat gcatcattgc cagagcgtcc tcaagcgaac cgcccgggtg
      841 ggctggttcg tgcagctttt tcagatattt acggtcactc ttcctcgaag aaaacaggag
      901 gtcaccgttt tcggtaagca catccagcgt gattaccaaa agctcatcgg gttttgcagc
      961 cgaggcttcg gggttctcag tgataaaacg atattccaaa ccggaagaag cttttttgaa
     1021 tgagtttacg gggtttttac atccccataa aaaaagcgtt aataacaata atatcagata
     1081 attacgcata tataattaat cattaacatt aattaactcg atgtcataca ccaaaatagc
     1141 gcggtaaggc actttgtggc catcgccggg aacgccgtaa gcaagatgcg ggtgaagaat
     1201 gaatcgtgct ttatctccta catgcaacat gcgaaggcct tcatcgatac cggtttcctc
     1261 tgtgttatgc ccaattagca tttcgcggtt tcctgtggaa tcagacgagt agataagggt
     1321 tccatcgagt aaataagtct tgtaagcgaa ttcggctttt tttccaacca cgggcattac
     1381 cccagacggt gaagtgtggt aaatctggta atatagtccc gttttggtct gctgcatatc
     1441 ccatttacgg cgcgttatat aacttttaat catttcgtcc tgcatatcaa tcaattccct
     1501 gttcatgtct ttcatatcct tgggggactt gacatcctgg tagctatagt tttcgttatc
     1561 aacagccaca ttgttgtttc ctgtattgca cgatacaata gcaagaaaag gaaacacaag
     1621 cattgcaaga agatatttgt tagaaaattt catttgtgta taaggcaatt ttattagtaa
     1681 aaaattcaac agcttcatca agtgagccat ggtattcgcc acctgcagcg ttaatatgtc
     1741 cgccaccttt aaaatactca gctgcaaatt ggttcacagc aaatttattt ttcgacctga
     1801 aagatacttt tatgaatgtt tttcgctcga taaaaatggc agatactttg atacctgcaa
     1861 ccgacagtgg ataatttaca aagccttcgg tgtcgccatc ctgatatccg taatcctgca
     1921 aatccttcag agtgagtttt atatatgcag ttttgagctc aggaagcaca accaaagcct
     1981 tattcaatgc atagcccaaa aggcgcatgc gtgaggctga aaaattatta tacacattat
     2041 cgaatgcagc atctttgttt accccgaatt tcaggatctt tgacacaata cggaaggtgt
     2101 tgctgttgga cgaattaaaa ttgaacgaac cggtatcggt catgatgccc acgtaaatgc
     2161 actctgcaaa gttgcggttt gcatatttca gaaatcctgc tttctcggta gcttcaaaaa
     2221 taagctctgc cgttgaactg ctttgcgtgg aatgaatgag caaattatag aaagcaaccg
     2281 gcccggggtg gtggtcgata agaattacat cggcaccggc ttttttaatc atgggttgta
     2341 tcactgccat acgggtgata tcgttgaaat cgacacaaat tattaaatct gcctcgctga
     2401 taagtgcttt tgctttctgt ttttctttgg aggctattac aatactatcg ttgccgggca
     2461 tccattttag gaatgccgga aaatcgtttg gacatacgac ttctgctttt ttttcaaggc
     2521 tgataaatag gtgatacata gccaatgccg acccaatggc atcgccgtcg ggattttcat
     2581 gcggaatgat taccgcatga tttgcccgca gcacatactc tttaaacttc actatgctgt
     2641 tgctgttttt cggaatcaat gccatttttc aaactcgtat tatgatataa cctataaaaa
     2701 tagaattttg tttcattagt ttacaaaaaa aagtaaaaat gattattaat tatactgggt
     2761 agttttaaga aacaaaactt caccatatat tataagctat gatacccaga gttgactcca
     2821 ttgtttacgt gtgtaaaatt caacccacaa aaaatggtga aaagaatagt tggttttacc
     2881 attttttttt gggtttaata gcatttattt atcatgaatt aacttcatat tcataacctg
     2941 atcgttgtgg cggatctgta acaggtacat tcctgcaaca tagcctgaca tatcaatagt
     3001 ttgtatgaat tcatccgata tataactatg gcattcccac aacaatttac cctgaatatc
     3061 ggtcaattca caataaatga gacctgtata acccgttatt tccagcgtag tttcagtgct
     3121 tgccggattt ggatacaagc taattccaat ggtattgtcg attccgttta tcgattgaca
     3181 aacttcaact ataaccatga tagtatctgt cgattcgcat tcacctacgc caacggtagc
     3241 ccaaacttca tactcaccgg gttctgccca ggttgcatta aagctttgtt ccgtggtttc
     3301 atcggaccag ataatataat cgtaagtgcc ggtaagcgta tagctaaaga gctcatcaac
     3361 acagcccggt agtgtttcag gaagtacaag ctcaggagcc gctatttcgg taacaacaat
     3421 ttcgtcggag gaactgcagt ggtattcgtt catgataatt accgagtagg ttccaccttc
     3481 atcaactacc aaaatttggt caacagagcc ggtgctccat tcgtaggaca ataagttgtc
     3541 gcctgcatca agggtgagag tctcgtaatc gcagatggtt tggtcttcgc caaggtttat
     3601 ttccggaacc gggttgaaga aaacattgat ttcatcggat gcagtacatc cgtgaatatc
     3661 tgttactgta acataatagt cgccatcttg agttgcctca taaatctggt cttcgtagcc
     3721 atcatgccat aaataactgc taaaatcttc tcctgcatta agatttacgg tattgccaat
     3781 acagtcttcg atatcgtctc caagttggat aacaggatta ttaaacactg ttatttgaag
     3841 tgtttctaca tcagctccat caaggttttc aacagtgagg cttacagtgt aaatgcccgg
     3901 tgtttcaaag gtgaaagacg tgttggcagt gttggcagaa ttctctccat caaaattcca
     3961 taaccatgtg agcgggttgt ttgttgatgc gtcagtaaat gttactgtaa gcggggcgca
     4021 tcct
//
LOCUS       DAOOQP010000183         4018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_26675_length_4018_cov_8.618723, whole genome shotgun sequence.
ACCESSION   DAOOQP010000183 DAOOQP010000000
VERSION     DAOOQP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26675_length_4018_cov_8.618723"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PLK75_12455"
     CDS             complement(<1..1696)
                     /locus_tag="PLK75_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40101.1"
                     /translation="MQIPLNQFEQVIDEDILKRGLSYFKKGRVYEPEEISPDEFETIV
                     EGSEDYVVRYKIKNDIIIEFVCSCPFDSGPVCKHVAAVIFYLQQDQLELNKKSVMTRR
                     NQNHGSAKRKTIAEQINEMLEKASHEELKQFIRDITERNISFRNAFLMSFAQHNSHES
                     KEVYAKQVKSILSNAAGRDGFINWSASRLVGNAINNLLRSAEKQVESNNYRSAVFIST
                     AVMEQMTQALQFADDSNGDIGGSIDAAYDILWDIAHRPLPEDIRKQILEYCFQAFDKQ
                     IYSGWDWHLGILNIASELAHTKEDIERIFKQIESSNESDYEKEKTQELKYRLILKTQG
                     QNAASNFIEQHISNPCLRREAIVNAIENKDFEKANMLANDGVKHDLKEKPGLAKEWYD
                     WLLRIAQKQKNTEKVIEYARMLLIDNFMPLQDYFQILKENVSHEQWNEFVEEVIKDIS
                     KTKRWSNSGLIESILIKEEQWDKLLEQVKRHASLNSIEYYEKYLAQKFPDEVVELYAH
                     GIIEYVECNVGRPHYKQACRFIRKIIKLGARDKANEIISSLRKQYPQRKALMEELDK"
     gene            complement(2024..2314)
                     /locus_tag="PLK75_12460"
     CDS             complement(2024..2314)
                     /locus_tag="PLK75_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY40102.1"
                     /translation="MSTKKQIIFPKHKKFLEIFGENMKLARKRRRLTMVQVAERADIS
                     RNTLYLIEKGSSGVSLGAVFNVLRVLGLENDILKIAADDELGRRLMDMELLK"
     gene            2990..3427
                     /locus_tag="PLK75_12465"
     CDS             2990..3427
                     /locus_tag="PLK75_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40103.1"
                     /translation="METVVKKNGKATQNDVAIVSKTGIAIDKVKPETAVAEVKQPEPV
                     QVLAPVQPVEPPKPVLTLEQKIEKVETLKTLIEKRERLEASRKKLTAFVVGANQFSEN
                     IVLTDENGNTFKTSNTEVFAKVVDTIQNTLVSKIAEIENQIEF"
     gene            3481..3621
                     /locus_tag="PLK75_12470"
     CDS             3481..3621
                     /locus_tag="PLK75_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40104.1"
                     /translation="MKILSFIFNKAKAKKSSDLELLIAKNPEVKNLIKKLDLVVIKTS
                     KS"
     gene            3618..>4018
                     /locus_tag="PLK75_12475"
     CDS             3618..>4018
                     /locus_tag="PLK75_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HOY40105.1"
                     /translation="MNTNEIYERITNTIIELLEEHKASDYSKSWYSLSGELFARNIVN
                     KHVYNGINQLLLSFIKRKRQYPCNRWLTFKQMEKYNARIIKGSKAAMVVYTSVIYIDE
                     DTGRNITHTVEQLLQHGQSIEHLNLRKIGYL"
BASE COUNT         1152 a          837 c          757 g         1272 t
ORIGIN      
        1 ccttatccaa ttcttccatc agcgcttttc gttggggata ttgttttctc aaactagaaa
       61 taatttcgtt tgctttatct cttgcaccaa gcttgataat ttttctaata aaccgacaag
      121 cctgcttgta atgaggtctc ccaacattgc actctacata ctctataatg ccgtgtgcat
      181 ataattcaac aacctcatct ggaaattttt gggcaaggta tttttcgtag tattctatag
      241 aattaagact cgcatgtcgt tttacctgtt ctaaaagctt atcccactgc tcttctttta
      301 ttaagatgct ttcaatcaag cctgaattag accaccgttt tgttttagaa atgtccttta
      361 ttacttcctc aacgaactca ttccattgtt catgagaaac attttctttt agtatttgaa
      421 aataatcttg taaaggcatg aaattgtcaa tgagcagcat tcgggcatat tcaatcactt
      481 tttcagtatt cttttgcttc tgtgcaattc tcagtagcca gtcgtaccac tctttggcca
      541 gtccgggctt ttcttttaaa tcgtgtttca caccgtcgtt tgccaacata ttggcttttt
      601 caaaatcttt gttttcaatg gcatttacaa ttgcttctct tcttagacat ggatttgaaa
      661 tatgttgctc aataaagttt gaagctgcat tttgtccttg ggttttcagt ataaggcgat
      721 actttagttc ctgagttttt tctttttcat aatctgactc gttagaactt tcgatttgtt
      781 taaaaatacg ctcgatatct tctttggtat gtgctagttc tgaagcaata ttcaatatac
      841 ctaaatgcca atcccaaccc gaataaatct gtttgtcgaa tgcctgaaag caatactcaa
      901 gaatttgctt tctgatatct tcggggagtg gtctatgagc aatatcccaa agaatatcgt
      961 aagctgcgtc aatactcccg ccaatatcac cattgctgtc gtcagcaaat tgcagagcct
     1021 gagtcatttg ttccattact gccgtcgaaa taaatacagc acttctatag ttgttgcttt
     1081 caacctgttt ttcagctgat ctcaaaagat tattaattgc attccccaca agccttgatg
     1141 ctgaccaatt aataaatcca tcccttccgg cagcgttact cagtattgat ttcacttgct
     1201 ttgcgtaaac ttctttcgat tcatgggaat tgtgttgtgc aaacgacatt aaaaatgcat
     1261 ttctgaaaga aatatttctc tctgttatat cgcgaataaa ctgtttcagt tcttcgtgac
     1321 tcgctttttc gagcatttcg tttatttgtt cagctattgt tttgcgtttt gcggaaccat
     1381 gattttggtt tcttcttgtc attactgatt ttttgtttag ttccagttgg tcttgttgta
     1441 aataaaaaat tactgcagca acatgcttac aaactggtcc tgaatcgaat ggacaactgc
     1501 aaacaaactc gataattata tcgttcttta ttttgtagcg cacgacataa tcttcggagc
     1561 cttcaacaat tgtttcaaat tcatcagggc taatttcttc cggctcataa acgcgtcctt
     1621 ttttgaaata cgacaaacca cgtttcaata tgtcttcgtc aatgacttgc tcgaattgat
     1681 ttaaaggtat ttgcatctta attattttta ttttttattg actttgaatt tattctgaat
     1741 aaaatcgcca gcaattttga atatgataaa cattttggca tgctgaaagc ggtactataa
     1801 tgtttgatat tgtttacgaa tccatcccca aacctaacct caatttccca ataatccaaa
     1861 gcaaaaaaca atatctgaga tagatttttc attggcagtt gttttgcaca gtttctcaag
     1921 cgtttttgca cccgtgttgc tttcaaaatg tgttggtttg cgctactgaa tccgatagat
     1981 gatgtaattg ataacgcgaa ttggttggta aaggggtagg gggttattta agtaattcca
     2041 tatccatcag tctcctacct aactcgtcgt cagcagctat ttttaagatg tcgttttcga
     2101 gccccaatac acgcaataca ttaaataccg ccccaagcga tacaccggag cttcctttct
     2161 cgatgagata cagggtattg cgactgatgt ctgccctttc ggcaacctga accatggtaa
     2221 gcctgcggcg cttgcgtgcc agcttcatat tttcaccaaa tatctcaagg aattttttat
     2281 gctttgggaa tatgatttgt tttttcgtgc tcataacgta ctataatatg tgcaaatata
     2341 atctaaatgt ttgtaatata gtacaaagat tgttgaattt tgtaagtcat gtttaaacct
     2401 ttattttcaa atgaatttta gattcaaaat gaatgaacct cggttctatt tgaggtcgtt
     2461 atatgtcagt agagctggct ttttaatcca agacaaaagc caacatctga aatagatttt
     2521 tcataggcag ttgttttgta cctgtttctc aagcgttttt gcacccgtgt tgctttcaaa
     2581 atgtgtgggt ctgcgctgct gagtccggtc ggtatgtgtc tgcaaagctg ggaaaccgaa
     2641 ctgcccctca caacatagga taaatagata ttcctaagtg ttcagcttgc tgcgtcaggc
     2701 tttttcgtga tttcattctt cgtgtatgag ttgtttcggc tgaacaccaa ggactatctc
     2761 tgttgctgag ggtcagttgc ggcttccctg atcgcagcag aacatactcg acgaactcat
     2821 cagatgcatt atggaacatt ttgaaaaaat acagctatgt gcaaaaaagc agaaacaggc
     2881 agcaggaaaa aacctgaacg cctctcaaaa tctatcatct gcatttgtgt gcgggctgtc
     2941 gggtgcggac aaaaaattca tgtattcatt aaccattaaa tcttgtatta tggaaactgt
     3001 agttaaaaag aatggaaaag caactcaaaa cgacgttgca atcgtgtcga aaaccggaat
     3061 cgctatcgac aaagtgaaac ccgaaacggc tgttgccgaa gtgaaacaac ccgaaccggt
     3121 acaggtactc gctccggtgc aaccggtgga accgcccaaa cctgtgttaa ccctcgaaca
     3181 gaagattgag aaggtggaaa ccctcaaaac cctgattgag aaacgagaac gccttgaggc
     3241 aagccggaag aagttaaccg cttttgttgt gggggcaaac cagtttagcg aaaacatcgt
     3301 gctgacagac gagaacggca acacgtttaa aacctcgaac accgaagttt tcgccaaggt
     3361 ggtagatacc attcagaaca ccctggtaag caaaattgcc gagattgaaa accaaatcga
     3421 gttctaaacc atcggggcag gtccggttca tcccggggct gccccttaat tttttgcatc
     3481 atgaaaatac tgtcattcat attcaataag gcaaaagcaa aaaagtcgtc agaccttgaa
     3541 ttgctgattg ctaaaaatcc cgaagtgaaa aacctcatta agaaattgga cttagtagta
     3601 atcaaaacct caaaatcatg aacaccaacg agatatacga acgcatcacc aacaccatta
     3661 tagagctgtt ggaagagcac aaggcaagcg attattcgaa gtcgtggtac agtctgtcgg
     3721 gagaattgtt cgcccgaaac attgtaaata agcatgtgta caatggcatt aatcaactgt
     3781 tgctgtcatt catcaagcgc aaacgacaat atccctgcaa ccgctggctc actttcaagc
     3841 agatggagaa gtacaacgcc cgtatcatca aaggcagcaa ggcagcgatg gtggtttata
     3901 cttctgtgat atatattgat gaagatacgg ggcgcaacat cacccacacc gttgaacaac
     3961 tgctgcagca cgggcagagc atcgaacacc tgaacctgcg aaaaatcgga tacctgaa
//
LOCUS       DAOOQP010000184         3906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_27663_length_3906_cov_6.099714, whole genome shotgun sequence.
ACCESSION   DAOOQP010000184 DAOOQP010000000
VERSION     DAOOQP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27663_length_3906_cov_6.099714"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            480..1127
                     /locus_tag="PLK75_12480"
     CDS             480..1127
                     /locus_tag="PLK75_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40106.1"
                     /translation="MKKFFSLFIVFSIICVAVHAQVGQATTIYNGKLLFVKTIPSSAY
                     TVVGKTKVSNSNKDEAAAGMDPSGIKEVLVAVDKSIKKETKGKQAAFDGVIIHSPNKI
                     ELIKFKQGTLAENTACTVISKDYKKKCGKKDIYFLNKPTVAYTEVKEIEVKNFTGFGQ
                     MKMGKDDIDNFFNKLYERSCKEADDGVDFDAILMVDDNIAVRGYFEAKTLKLIKY"
     gene            1315..2553
                     /locus_tag="PLK75_12485"
     CDS             1315..2553
                     /locus_tag="PLK75_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY40107.1"
                     /translation="MINFEKYSLSNGLKVILHQDLTTPMVVVNVMYDVGARDENPERT
                     GFAHLFEHLMFGGSVNVPEYDRIVQKAGGENNAFTNNDITNYYVSLPSVNLEAGLWIE
                     SDRMLELAFSEKSLEVQRNVVVEEFNQRYLNQPYGDVMLHLRPLAYCVHPYRWNTIGE
                     KPEHIRNAVLEDVKEFFFAHYAPNNAILVVAGNFEPKGTRKLIEKWFGTLPARPTAAR
                     KLPIEPLQTEPRIINLERDVPQNALYRAYHMVKRLHRDYYATDLISDILSNGRSSWFF
                     ERLIKDYKLFSEIDAYIMGSIDPGLFLISGKPLPGKTYNECEAAIDEQLQMIISGSFT
                     DRELEKVKNRCIAARKLEWAGVLNKAIDLAYFELLGNAAMINAEEENYRSVTRKQVME
                     VAAALFSKQNCSTLYYNKTH"
     gene            2555..3835
                     /locus_tag="PLK75_12490"
     CDS             2555..3835
                     /locus_tag="PLK75_12490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY40108.1"
                     /translation="MSNFRIEGAPIYNFDARVLNRATARTLPCGIPVYHIGGNTMNVV
                     RIDFLFDAGSMYQNDKLVPQHCLSLLREGSENYSASGIADILDYHAVFSELSFNRNKA
                     GMGFYIDIASAAKVLPAITDFIFKPTFPQNELDVFNQNSFQKLKIEFEKVKFLAEKRI
                     FSKIYGLEHPLGAEIEPNDVFNVTRTQMMEFHRLHYSTGNCCIVVAGDTSEDLLNMLN
                     EALYPFCIDEISQIAKPEYSIKSSRDKFEYIQKPSATQAAIRMACLIDYDSFADYHML
                     KVLNTLLGGYFGSRLMKNIREDKGLTYGIGSSVQLSEGKALFSISAEVAGNMWQEALN
                     EINFELKSLSEMPISDEELNLVKKTMLGDKLAMFDGAFASSAVFRSLLEAGLDYTFVE
                     DSIHAIHQADENRLKTIASSMLQPEKMTVVVAGP"
BASE COUNT         1280 a          701 c          781 g         1144 t
ORIGIN      
        1 ttgtcatatc atcacaaatt tttaagaatg caaaaataat aaaaacaggg aatttaatat
       61 gagtatgcaa gaatcaatta tgctgaaata tgctcaaacg gtatgatatg aagttaataa
      121 gcatattact tgaaacgtac tttatttcgt atctaaaaaa caaaatttcc ggccagctat
      181 tccgcaaatc aattacgttt aacattatta ccaagcacct gctaattaaa attatttaca
      241 aaacatattt ttatacattc aacatatttt taaatttttt cagtaattat tgttacatta
      301 taatattggg ataattcgca atcatcgcga ttaaaaacaa agaagtatta attaatcatg
      361 ctgttaagaa ttttattaag tacatttgac ctgactgttg agagcaatta tgggttcttg
      421 agtttttgac agtcaatttt taatagtaac agaacccaaa acatattaag catttaccta
      481 tgaaaaaatt ttttagtctg tttattgttt tcagtattat ttgtgttgcc gttcatgctc
      541 aggtcggtca ggcaaccacg atatacaacg ggaaactcct gtttgtaaaa acaattccct
      601 ccagtgctta tactgtagtg gggaaaacta aggtatcgaa cagtaataaa gatgaagctg
      661 cagccggtat ggatccgtct ggcattaaag aagtgcttgt tgccgttgac aagtcaataa
      721 aaaaagaaac taagggaaag caggctgctt ttgatggagt gattattcac agtccaaata
      781 agattgaatt gattaaattt aaacagggta ctctggctga aaacactgct tgtactgtga
      841 tttcgaaaga ctataagaaa aaatgcggca aaaaggacat ttatttcctg aataaaccaa
      901 ccgttgccta taccgaagta aaagaaatag aagtaaaaaa ctttaccggt ttcgggcaga
      961 tgaaaatggg aaaagatgac attgataact tcttcaataa actgtatgag agatcctgca
     1021 aagaagctga cgacggtgtt gatttcgatg caatactgat ggttgatgac aatattgctg
     1081 tgagaggata tttcgaagca aaaacattga aactgataaa atattaatta atacagtgaa
     1141 caacgaaaac tgcaaccgca aaaaacttgc ggttgttttt tttcgctcaa cattttcaaa
     1201 atcgtttctg atattttcat ttctatatag aagtccgaaa taattaagaa ctatttttca
     1261 ggtgtttcaa tcagggagta ttgttttttt atctttgctt catatattca tgcaatgata
     1321 aatttcgaaa aatacagcct ttccaacgga ctaaaggtga tccttcacca agatttaaca
     1381 acaccaatgg tggtggttaa tgtaatgtat gatgtgggag ctcgtgacga aaatcctgaa
     1441 agaactggct ttgcccacct gtttgaacac ctgatgtttg gtggctctgt caatgtacct
     1501 gaatatgacc gcatcgtgca gaaagccggt ggtgaaaaca atgcttttac aaacaacgac
     1561 attacgaact attacgtcag ccttccatcc gtaaaccttg aagccggttt atggattgaa
     1621 tcagaccgta tgcttgaact ggcattctcc gaaaaaagcc tcgaagttca gcgtaatgta
     1681 gtggtagaag aatttaacca gcgttacctg aaccaacctt atggagatgt gatgttgcat
     1741 ttgcgccccc ttgcgtattg tgtgcaccca taccgatgga acacaatcgg agagaaaccg
     1801 gaacacatcc gtaatgctgt actggaagat gtaaaagaat tcttttttgc gcattatgct
     1861 ccaaataatg caatacttgt ggtagcgggc aattttgaac ccaagggcac ccgaaaactc
     1921 atcgaaaaat ggtttggcac cttacctgca agaccgactg ctgccagaaa attgccaata
     1981 gaaccgttgc aaacagagcc acgtataatc aatctagagc gcgatgttcc acaaaatgca
     2041 ctatacagag cctatcatat ggtaaaacga cttcatcgcg attattatgc aaccgattta
     2101 ataagtgata tcttgtcgaa tggcagatct tcgtggtttt tcgaaagact cattaaagat
     2161 tataaacttt tttctgaaat tgatgcgtat atcatgggaa gtatcgaccc cggactgttt
     2221 ttaatatcag gcaaacctct tccgggtaaa acttacaatg aatgcgaagc tgctattgat
     2281 gagcaattac aaatgataat atcaggtagc tttaccgaca gggaactcga aaaggtaaaa
     2341 aaccgctgca ttgcagcacg aaaacttgaa tgggccgggg tgctgaataa ggctatcgat
     2401 ctcgcatatt ttgaattgct tggtaatgcg gctatgatta atgctgaaga agaaaattac
     2461 cgcagcgtaa cccgaaagca agtgatggaa gttgcagccg cgcttttttc aaaacaaaac
     2521 tgcagtacgt tgtattacaa taaaacacac tgacatgagt aatttccgta tagaaggagc
     2581 tccgatatat aatttcgatg caagggtgtt gaatcgtgca acggcaagaa ccctgccctg
     2641 cggcattcca gtatatcaca ttggcggcaa taccatgaat gttgtgagaa ttgattttct
     2701 ttttgatgca ggttccatgt atcaaaacga taaacttgtc ccgcagcatt gtctatcgtt
     2761 gctgcgtgaa ggttccgaga actattcggc atccggaatt gcagacattc tcgactatca
     2821 tgcagtattt tcggaactgt cgttcaaccg caacaaagca gggatgggtt tttacataga
     2881 tatagcatcg gcagccaagg tgctacctgc aattaccgat tttattttca aacccacgtt
     2941 tccacaaaac gaactcgatg ttttcaacca gaacagtttt cagaaattaa aaatagagtt
     3001 tgaaaaagtg aaatttctgg cagaaaaacg gatattttct aaaatatacg gacttgaaca
     3061 tccactggga gctgaaattg aacctaacga tgtgttcaat gtaacccgca cccaaatgat
     3121 ggagttccac cgattgcatt attcaacagg gaattgctgt attgtggtag ccggtgatac
     3181 tagcgaagat ttattaaata tgctgaatga agcattgtac ccattttgca tagacgaaat
     3241 ctcacaaatt gcaaaaccag aatatagtat taaaagttcc cgtgacaaat tcgaatatat
     3301 tcaaaaacct tctgcaacac aagctgccat tcgaatggct tgtctcatcg actatgacag
     3361 ttttgccgat taccatatgc ttaaggtatt gaatacactg cttggcggat attttggatc
     3421 gcgattgatg aaaaacatca gggaagacaa agggttgacc tatggtattg gttcatcagt
     3481 gcagcttagc gaaggcaagg ctcttttcag tatttctgcg gaggttgcag gaaatatgtg
     3541 gcaagaggct ctcaacgaaa taaatttcga gctgaaatca ttaagtgaaa tgccaatttc
     3601 tgacgaagaa cttaacctcg taaagaaaac catgctgggc gataaactgg ctatgttcga
     3661 tggggcattt gcttcgtcgg cagtattcag aagtttgctg gaggcaggtc ttgattatac
     3721 gtttgttgaa gatagcattc atgcaattca tcaggctgat gaaaatagac ttaaaactat
     3781 tgcttcatca atgcttcaac cagaaaaaat gactgtagtt gtagcgggac cataatttct
     3841 ctaatttaac tttagtagtg gtgcgttaat tttttaatca aatagtgtag gttgttcttt
     3901 gacgtt
//
LOCUS       DAOOQP010000185         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_28289_length_3843_cov_6.244192, whole genome shotgun sequence.
ACCESSION   DAOOQP010000185 DAOOQP010000000
VERSION     DAOOQP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28289_length_3843_cov_6.244192"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLK75_12495"
     CDS             <1..824
                     /locus_tag="PLK75_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40109.1"
                     /translation="KLTGVIGPGGFWNLFSLICLTSIAPFAMPQLVQKFYAIRDQRSV
                     KIGMFASTFFALIIGGIAYFIGSTTHVFFSIEKTPHLFNNGIPIYDKLMPELLTSVIP
                     NSLVVVVLILILAASMSTLASLVLISSSSIAKDLYAGFINRNASDKRLTMLMRIMSGV
                     FIILAVLLALVEFDVIVEILGISWGAIGSFFLGPFIWGLFCKKVNRLGAVSSAIFGLG
                     TCLTLYFIGHSIPKGEVVAWYVTAPGAGTIGMFVSLLVNPIFSLFCCKGNCKVHG"
     gene            complement(972..2135)
                     /gene="dinB"
                     /locus_tag="PLK75_12500"
     CDS             complement(972..2135)
                     /gene="dinB"
                     /locus_tag="PLK75_12500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOY40110.1"
                     /translation="MSERTIAHLDLDTFFVSVERLINSRLNHLPVIVGGGSDRGVVSA
                     CSYEARKYAVHSGMPMKMARQLCPDAVVIRGDMDTYTRYSRMVTDIIAESAPVYEKAS
                     IDEHYLDLTGLDRFFGMQQWAHELRSRIIKETGLPMSLGMSVNKTVAKIATCESKPCG
                     EMYVQPSGIPGFLDPLSIRKIPMLGKQSYRLLRSMGVPDIRTLKLIPPEMMEKVLGKN
                     GRSIWEKANGIDPTPVIQYSERKSIGSEHTFEHDTTDVACIRSLLSGSVEKLCFKLRK
                     DQWLVSCITVKIRYSDFNTFTLQKRIAYTSFDHTIMKTTLELFEKLYNRRMLIRLVGV
                     RFSGLIRGTQQLNLFEDTPEMVNLYIAMDNIRKRFGTSALKRAGGIWQGRVSY"
     gene            2264..2953
                     /locus_tag="PLK75_12505"
     CDS             2264..2953
                     /locus_tag="PLK75_12505"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HOY40111.1"
                     /translation="MNTKRLIQIVILVSVVVSISAGIGGFYYIVDKHPWIVQKFKQIF
                     TCTEKDEKESSSKIKNDSYQLHRKAAKQTGLKAIQNDEQLNSYKKEGKLIQVNNLKGY
                     KIGKLTHSKAVLTPETYKTLQEIGLRFSQKAGNGNYFVVTSMTRTVKDQKKLTKNNVN
                     ATKNMSTHCYGCSFDISYIRFNGKKGNNKKLQDDLESILIDYQKDKRIYIVVEKRMHC
                     YHITVIPTLKT"
     gene            3050..3137
                     /locus_tag="PLK75_12510"
     tRNA            3050..3137
                     /locus_tag="PLK75_12510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3084..3086,aa:Ser,seq:cga)
     gene            3374..>3843
                     /locus_tag="PLK75_12515"
     CDS             3374..>3843
                     /locus_tag="PLK75_12515"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HOY40112.1"
                     /translation="MENKNSFSLMFFAIGSRANENGEIPLHLRITVNNRKVEMSLHRR
                     VDKSIWNPSAGMAMGNTKNAKIINSFLMSVQTTVYEHFKYLRETGRPITAEALRNAYL
                     GIEEEEKGPKVLELFKEHNDKLRLLEGIDYAPLTIERYETSYKHTQDYIRAKYN"
BASE COUNT         1112 a          841 c          835 g         1055 t
ORIGIN      
        1 caaagcttac cggtgtaatc ggccccggcg gattttggaa tttattttca cttatctgcc
       61 ttaccagtat cgctcctttt gccatgcctc agttggtgca aaaattttat gcaatacgcg
      121 accaaagatc ggtaaagata ggaatgtttg cttctacgtt ttttgctttg ataattggcg
      181 gtatcgctta ttttatcggt tcaaccacac atgttttctt ttcgattgag aaaaccccgc
      241 atttgttcaa caacggaatc cctatctacg acaagttaat gccggaattg cttaccagtg
      301 tcattcccaa ttcgctggta gtagtagtgc tgattctaat tcttgctgcc tcaatgtcga
      361 cgcttgcttc gctggttctg atttccagct cttcgatagc aaaggactta tatgcgggct
      421 ttatcaaccg taatgctagc gataaaaggc tgactatgtt gatgcgaatt atgagcggtg
      481 tgtttatcat tcttgccgtt ttgcttgctt tggttgagtt tgatgtaatt gtcgaaatat
      541 tgggtatttc gtggggagct atcggatcgt tctttttagg cccgttcatc tggggacttt
      601 tctgcaaaaa agtaaaccgg ttgggagccg tatcttctgc aatttttgga cttgggacat
      661 gcctgacatt gtattttata gggcacagca taccgaaggg agaagtggta gcgtggtatg
      721 taacggcgcc tggagcgggt actatcggca tgttcgtttc gctgttggta aacccgattt
      781 tcagcctatt ctgctgtaaa gggaactgca aggttcatgg ataaaccgag ccgttcatcg
      841 agcgtttccg agatgccggc tcgataatcc acgttaataa aacttgtgat ccctgaaatg
      901 ataatgcaaa agtttgaaag atgtcgcagg tttacaaacc gtttcggaca cctcgactac
      961 ggtgtccgaa actaataact cacccttccc tgccatatcc ctcctgccct tttcagtgct
     1021 gaggtaccaa agcgtttacg gatattatcc atagcgatat acaggtttac catttcgggg
     1081 gtatcttcaa acagattcag ttgctgggtt cccctgataa gtccgctgaa gcgcactccc
     1141 accagcctga tgagcatgcg gcggttgtag agtttctcga acagctccag cgtggttttc
     1201 attatcgtgt ggtcgaacga agtgtaggca atacgcttct gcagggtaaa ggtgttgaaa
     1261 tcggaatagc gtatcttcac cgtgatacac gacaccagcc actggtcttt acgcaatttg
     1321 aagcacagct tttcgaccga acccgacaaa aggctgcgga tgcaggccac atcggtggtg
     1381 tcgtgctcaa aggtatgctc gctgcctatc gacttacgct cggaatactg aatcacaggc
     1441 gtagggtcga tgccgttggc cttttcccag atgctgcgtc cgttcttgcc cagcactttt
     1501 tccatcatct caggcggtat cagcttcagc gtacggatat cgggaacacc catcgagcgc
     1561 agcagccggt aggattgttt gccgagcatg ggaattttcc gtatcgaaag cgggtcgaga
     1621 aagcctggaa tacctgatgg ctgcacatac atctcgccac agggtttcga ctcacaggtg
     1681 gcaatcttgg ccacggtttt gtttaccgac atacccaacg acataggcag accggtttct
     1741 ttaataatgc gagacctcag ctcgtgcgcc cactgctgca taccgaaaaa gcggtcgagt
     1801 ccggtaaggt cgagataatg ctcgtcgata ctggcttttt cgtacaccgg tgcactttcg
     1861 gcaataatgt cggtaaccat gcgcgaatag cgggtgtaag tgtccatatc gccacggata
     1921 acaacagcat ccggacaaag ctgcctggcc attttcatgg gcatacccga atgcaccgca
     1981 tactttcgtg cctcgtagct gcacgccgac accacgccac ggtcggagcc accgcctaca
     2041 atcacgggca ggtggttcaa acggctgttt atcagccgct ctaccgacac aaaaaaggtg
     2101 tcgagatcga gatgggcgat agtacgctcg gacattttgt ttaattttta agcacaacta
     2161 cgattacaat ataatcattt atttttcata aaacaacttt catgaatttt cctaataatt
     2221 tatattttta caaaagagtg aaacctgttc agataaattg aagttgaata cgaagcgcct
     2281 aatacaaatt gtaatacttg tttcggtagt agttagtatc tcggcaggta ttggcggatt
     2341 ttactacatt gtagataagc acccctggat agttcaaaaa ttcaaacaaa tttttacctg
     2401 taccgaaaaa gacgaaaagg agagctcaag caagattaaa aacgacagtt accaattgca
     2461 tcgtaaagct gcaaaacaga cgggattaaa agccatacaa aacgacgagc aactcaacag
     2521 ctataaaaaa gaaggcaaat taatacaggt aaacaaccta aaaggttata aaataggcaa
     2581 actcacccat agcaaagctg ttttaacacc ggaaacatat aagaccctac aggaaatcgg
     2641 gctcaggttt tcgcaaaaag caggcaatgg caattacttt gttgtaacat caatgacacg
     2701 cactgtgaaa gatcagaaga agctcaccaa aaacaatgtc aatgctacaa aaaatatgag
     2761 tacgcattgc tacggatgct ccttcgatat ttcgtacata cgttttaatg gcaaaaaagg
     2821 caataacaag aagcttcaag acgatttaga aagcatttta attgactatc agaaagacaa
     2881 aagaatttac atcgtcgttg aaaagcggat gcactgctac catatcactg taattccaac
     2941 actcaagaca tagttgcaac aaacaaaata ttttcgatat aaaaccggca gaagcgataa
     3001 tttgattttg tagtttcgct ataatgtgta cttttgcaca gaattttttg gagagatgcc
     3061 agagcggtcg aatggggcgg tctcgaaaac cgttgtccgc tttccgcgga cccagggttc
     3121 gaatccctgt ctctccgctg agacgaagtg caaaacgatg taaaagcctg aaaacaagtg
     3181 ttttaggctt ttttctttgg tgcgtaaaag tgcaatttta gcctatttta aactatagtc
     3241 cattgactaa tcgttgacaa tcatttttaa ttttttttgt caacggtcgg tgcccctttg
     3301 cactgatttg caacactttg cattttgaaa actgctccat aatgtgttct tttataactt
     3361 aaatttatgt attatggaaa acaaaaattc ttttagttta atgttctttg caatcggcag
     3421 tagagctaac gaaaacggtg aaataccatt gcatctgcgt attaccgtca acaacagaaa
     3481 ggtcgaaatg tcgctgcacc gacgcgttga caaaagcatc tggaatcctt ctgcagggat
     3541 ggccatggga aacactaaaa atgcaaaaat catcaatagc ttcttaatgt cggtgcaaac
     3601 aacagtatat gagcatttta aatatttacg tgaaaccggc agacccatta ccgcagaagc
     3661 tctccgaaat gcatacctgg gaattgaaga agaagaaaaa ggtcctaaag tactggaact
     3721 gttcaaagaa cacaacgata aattaagact cctcgaggga attgactatg cacccttaac
     3781 tattgaacgg tacgaaacct cttataagca tacacaagac tatatccgag ccaaatacaa
     3841 taa
//
LOCUS       DAOOQP010000186         3811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_28595_length_3811_cov_4.776358, whole genome shotgun sequence.
ACCESSION   DAOOQP010000186 DAOOQP010000000
VERSION     DAOOQP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28595_length_3811_cov_4.776358"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="PLK75_12520"
     CDS             complement(<1..1847)
                     /locus_tag="PLK75_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40113.1"
                     /translation="MNKYSIISLTLFFIFTVNLGVTQENCSVLKPISEKEQIELAGMP
                     QLTLPSQYKNKTLPVEIDNSTLIYYPPIFLQAGLCCGQAAAIALGYTYEMCRLRNQDA
                     SNISNNFPTHFTWNWENGGFGYYGVSYYHSLEVIRLVGHPTTETYGGTYSFGGGSRWM
                     SGYDSYYSGMHNRLVNAYAIKCTDEEGILTLKNWLNDHLDGSATGGVAFFYSQYQSPS
                     NVLAAGTPHAGERVIVSWGSSPNHGMNICGYNDEIKWDYNGDGQYTNHIDINSDGVVN
                     VKDWEIGAFKMANTYSSPYFSWMMYKTLADNSSQGGIWNNTVNVVCGKESYEPLLTYK
                     INLYYTHRKRIKIRAGMSTNTLDAIPRYIIEYPIVNYQGDDMGMQGGNEEEQKYFEFG
                     LDVTPFLNYISPGSEAKFFFEIVEDDPDVWGSGNIISFSLMDYTGSLVETPCSQTNVP
                     IANNTKTQLSIIKTIAYDPVNITTDVLPEAEVYHDYSYQLEADGGTAPYRWEFDIDYT
                     VATGSASMPSDITTAFSGSSLELPFSFNFYGQEYNKIYVSNDGYIDFSGDPYSLPYQH
                     ELMTTFINRKCIAACMVDMNANVYYKLGADYVSIRWVKANYIEAAVKIH"
     gene            complement(1852..>3811)
                     /locus_tag="PLK75_12525"
     CDS             complement(1852..>3811)
                     /locus_tag="PLK75_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40114.1"
                     /translation="KDYYYIDVSDEAGLLMLKAWVYDHGRGEMPGGMATFMTNIGWGG
                     DNYLPQGTPEAGYYTITKCGDNPLHSRTVVGYNDNICWDYNNDGQYTNNIDLNDDGVI
                     NVRDYEKGGFKLAESFGPEWQGHGGYLYIMYKCLADEFPLGGILNNEMIVIEPWPDYQ
                     PKYTAKIKLNHEYRGKVKLSIGISTNPEATTPDYEMDFPTINYQGGNNYMKGGETEDD
                     KYFEIGYDLERLLSYMPAGHEFRLFLIGREIDPNNGYNGSIQEFTIYDYTSEPETSFT
                     WTGNVPFVNGIISIPLNLSLDFDKPAITTESLPIFSANTNFNYNIASEGGTLPFTWSL
                     EAVCNTTTSAYTYDYYTDTKLSPETYFDSIITVDLPFQFPYNGETYNKIIVHTDGYIM
                     VGNDQFPWPYAKDFFEDFIQMERVIGPHMRFDFVCNPAYNDGIWLKDYNDHISIRWNV
                     SQQYLESWTDNNFGLSLYENGKIDFFYGSGFYIQNSTGVSAVSYGNDKDYFIIKKDEI
                     PQSPQRINITLYPTPENIQISDNGVLSGNIAFTEPYPIRVCLTDDNNITCKKDYSLLT
                     DIMENDSEEIHIYPNPSDTYLVITGDKADSISAITLVNANGKQIDSYSFENISDVRTI
                     DIRSLSSGCYILKIFLPNDILFSPFIKN"
BASE COUNT         1046 a          786 c          772 g         1207 t
ORIGIN      
        1 ttatgaattt taacagcagc ttcaatatag tttgccttta cccaccgtat ggacacatag
       61 tcggctccca gtttataata tacgtttgcg ttcatatcga ccatacaggc tgcaatgcat
      121 ttgcggttga taaatgtggt cattagttcg tgctgataag gcaacgaata tggatcgccg
      181 ctgaagtcaa tgtacccatc attcgataca tatattttat tatactcctg accgtagaaa
      241 ttgaaactga acggaagctc aaggcttgac cccgaaaatg ctgtggtaat gtcggaaggc
      301 atcgaagcac tacctgtggc aacagtatag tcgatatcga attcccaacg atatggggct
      361 gtcccaccat cagcttcgag ttggtaggaa taatcgtgat atacttctgc ttcgggcaga
      421 acatcggtgg tgatgtttac tgggtcgtag gcaatcgttt taattatgct gagctgtgtt
      481 tttgtgttgt ttgcaatcgg cacgttcgtt tgggaacaag gtgtctcaac aaggctaccg
      541 gtatagtcca tcaacgaaaa tgagataata tttccgctac cccaaacatc tgggtcgtct
      601 tcaacaattt caaagaaaaa ctttgcttct gaacctgggc taatatagtt cagaaatggt
      661 gtcacatcta atccgaactc aaaatatttc tgttcttctt cgttgccacc ttgcatcccc
      721 atatcatcgc cctgataatt tacaatcggg tactcaatga tatacctagg aatggcatcc
      781 aatgtatttg tcgacatgcc ggctcgtatt ttaattcttt tgcgatgggt gtagtataga
      841 tttatcttat aggtgagcaa aggctcatac gactcttttc cacatactac gttcactgtg
      901 ttgttccata ttcctccctg acttgagttg tcggcaagag ttttatacat catccacgaa
      961 aaatatgggg agctgtaggt gtttgccatt ttaaaagcgc ctatttccca gtctttaaca
     1021 ttcacaaccc catcgctgtt tatgtcgata tggttcgtgt actgtccatc gccattatag
     1081 tcccatttaa tttcatcgtt ataaccgcaa atgttcatac catggtttgg agaggagccc
     1141 cacgacacaa tcactctttc accggcatga ggagtgcccg ctgctaaaac attcgatggg
     1201 ctttggtatt gcgagtagaa aaaggcgacc cctccggtcg cagagccatc aaggtgatca
     1261 tttaaccagt ttttaagcgt aagaatacct tcctcatcag tacatttaat ggcatatgca
     1321 tttaccaacc gattgtgcat tccgctgtaa taactgtcgt aaccactcat ccaccgactg
     1381 cctcctccaa atgaataggt gcctccgtat gtttctgttg tgggatgccc taccagcctg
     1441 attacctcaa gactgtggta atagctaacg ccataatacc caaatcctcc gttttcccag
     1501 ttccatgtaa aatgcgtagg gaagttattt gaaatatttg aagcatcctg attccgcagt
     1561 ctgcacattt catatgtata gccaagtgca atggctgcag cctgtccgca acaaagacct
     1621 gcctgcaaaa atatgggagg gtaatatatt agtgttgagt tgtcaatttc aacaggtaat
     1681 gttttattct tgtattgtga gggaagtgta agttgtggca taccggcaag ctcaatctgc
     1741 tctttttccg atatgggctt aagcacactg cagttctcct gcgtaacacc aaggtttaca
     1801 gtaaaaatga aaaatagtgt tagtgaaatg attgagtatt tattcatact tctaattttt
     1861 aatgaatgga gaaaaaagaa tgtcgttagg caaaaaaatc ttcagaatat aacaacctga
     1921 gctaaggctt ctgatgtcta ttgttctgac gtcagatata ttttcaaaac tataagaatc
     1981 aatttgcttc ccgtttgcat tgaccaaggt aattgcagaa atggaatcgg ctttgtcacc
     2041 tgttattaca agatatgtgt cggaagggtt gggataaata tgtatttctt cggaatcatt
     2101 ttccataata tcggtaagca atgaatagtc ttttttacaa gtaatgttat tgtcgtcggt
     2161 aaggcaaacc ctaataggat agggctcggt gaaggcaatg tttccactga gtactccatt
     2221 gtcagagatt tgaatgttct cgggagtagg gtatagggtg atattgatcc gttgtggtga
     2281 ttgcggtatt tcatcctttt taattataaa ataatccttg tcgttaccat acgaaacagc
     2341 tgaaacccct gttgaattct ggatatagaa tcctgaacca tagaaaaaat cgattttccc
     2401 attttcgtac agactcaggc caaaattgtt atcggtccat gattcaaggt attgttgcga
     2461 tacattccac ctgatactta tatgatcatt ataatctttt aaccaaatac catcgttgta
     2521 tgctggattg caaacaaaat cgaagcgcat atgaggcccg ataacccttt ccatctggat
     2581 aaaatcctca aagaagtctt ttgcgtaagg ccaagggaac tgatcgttgc ctaccatgat
     2641 atatccgtcg gtgtgaacaa ttattttatt ataggtttct ccattatacg gaaattgaaa
     2701 aggtaaatca accgtaataa tgctgtcgaa ataggtttca gggctaagct ttgtgtcggt
     2761 ataataatca taggtgtatg cgcttgtagt ggtgttacaa acagcttcaa gactccaagt
     2821 gaatggcagt gtgcctcctt cggatgcaat attgtaatta aagttggtgt tagcactaaa
     2881 tatgggtagt gattcggtag taattgcggg tttgtcgaaa tccagactga ggttcagtgg
     2941 aatagatatt atgccattaa caaaaggaac attgcctgtc caggtgaagc tggtttcagg
     3001 ttcgcttgta tagtcgtata tggtgaactc ctgaatcgat ccgttgtagc cattgtttgg
     3061 gtcaatttcc ctgcctatta gaaaaagcct gaattcatgg cctgctggca tataacttaa
     3121 caatctttct aaatcgtatc cgatttcaaa atacttgtca tcttcggttt cacctccttt
     3181 catgtaattg ttcccacctt gatagtttat ggtcgggaaa tccatttcat aatcaggagt
     3241 tgttgcttca gggttggtgc ttatgccgat actgagtttt acttttccac gatactcgtg
     3301 gttcaatttt atttttgcag tgtattttgg ctgataatca ggccagggtt caattacaat
     3361 catttcattg ttcagaatac caccaagtgg aaattcatct gccagacatt tgtacataat
     3421 atacaaatat ccaccatgtc cctgccattc aggaccaaag ctctcagcaa gtttaaagcc
     3481 gcctttttcg tagtctctta cattaattac tccatcgtcg ttcaaatcga tgttgttggt
     3541 atattgcccg tcgttgttgt aatcccagca gatgttatcg ttgtagccca ctacggttct
     3601 tgaatgtaaa ggattgtcgc cgcacttcgt tattgtataa taccctgcct cgggtgtacc
     3661 ctggggtaaa taattgtcgc caccccaacc gatattggtc ataaatgttg ccataccccc
     3721 cggcatttcc ccgcggccat ggtcgtaaac ccatgccttt agcataagta aacctgcttc
     3781 atcagaaaca tcaatataat aatagtcttt a
//
LOCUS       DAOOQP010000187         3745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_29261_length_3745_cov_4.116260, whole genome shotgun sequence.
ACCESSION   DAOOQP010000187 DAOOQP010000000
VERSION     DAOOQP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29261_length_3745_cov_4.116260"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3745
                     /locus_tag="PLK75_12530"
     CDS             <1..>3745
                     /locus_tag="PLK75_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40115.1"
                     /translation="AVMGVYGSTMTSGGSGYTYANVTFTGGSPTVPATATANIVGGAV
                     TSITITNPGSGYLSRPTVTISGDGSGAVYANATLKVVSINITNGGAGYAILPTFSFST
                     SGSSSGAAATCVTTAGVLTGYSGLTGGAGYTSVPTITITTGTETTYGVYIGNTGTVRL
                     HNNTISAIQIGNDIPEYRHTFYAITSSGTAGVREINNNNVGSSTANAIAVGTPSTTSG
                     ICAFYGISNSATGSCQMIENTVKNCIAYGTTASATNYGMQKSGNSASFDMSNNTVENL
                     SSTANATMIGLLSSNASVSGTISNNVVNNLNSATTTNSVRGISVSAAGNVYDNTVTNI
                     VSASLTTGYSVGMLLSNGTVVNVYDNTFNTVQGNALTTGYVAGISITGGAANEVYRNK
                     IYDISSSSSSMSTGTVNGVVVSGTTTPMASIIYNNLIGDVKAPSASSSTDVVRGISVI
                     NTGTTSSTKLYYNTVNLNASSSGTNFSSSGIYHTTQTTATRASLEMINNLIVNESTPK
                     GTGKAVAYRRSSTTLNNYSTASNNNSYYAGTPGSSRLIFYDGTNSDQTIEAFKTRMST
                     RDQASVSNDAVFLSSTGADATFLHLDNDDNCRIDGKGKVIATYTSDYDSDPRDASYPD
                     IGADEVTTGYPANLATSPTTLPTNAYVWTGQTGTDTWATSSNWVQYNGSTFSIPGSAP
                     NATNANVYIRTYNTCVLENLPTVTVSSDFNCGNLIIESSGGLTVTGANKLIVNGYLTN
                     DGNLTASGSPTIQVNGNWTNNGTFTPATSTVLLGGTGATINTGGVGTGKSFYNLTVTK
                     TSGTATISTNNLDVDGNLSITSGTLSLSGSLSATIAGNWSHTGGNFTCNSSTVTFDGT
                     NSTITTTSTGTKSFYHVIIDDAAVLGSDVDINGNLTINNGKSFSDGSFYSMTLAGNWT
                     NNGTFTAGSVGTVTFDGSASAIIGGSSSDTFFKMALNKGSGLGLMLEVNVPITLSNTS
                     TSALTMTNGLLKINSDGELNLPAAGQTIGTGAGIEVNGGTLNGGSATMVNNGYFKLTS
                     GTVNIGAVAGNSFENRSNSRFYIESGIMNITGRLMFTAANTDAIISGGTLNLCTVGNT
                     SSSYGAFNMIGTANISITGTPLITFQNAGSGATPLDLNILSGTGTKNFSGGTLQFGNA
                     STPAGQNFKIKSEVPVFNIVVNGNNNPTATLTANLPGSNNVTINSGGTLNTSSYQLTL
                     SGDWTNNGTYTASSGTVNFAGTSQQEIQAGTGAFNNVIFSNTTT"
BASE COUNT         1128 a          784 c          819 g         1014 t
ORIGIN      
        1 gctgtaatgg gtgtgtacgg aagcacaatg acaagtggtg gtagtggtta tacgtatgcc
       61 aatgtaactt ttacgggtgg gtcaccaact gttcccgcta cagcaactgc aaatattgtt
      121 ggaggtgcag taacttcaat cactattacc aacccgggca gtggttattt atcaaggcca
      181 acggttacta tttcgggtga tggcagcggt gctgtatatg ccaatgcaac acttaaagta
      241 gttagtataa atattaccaa tggcggtgcc gggtatgcta ttttgccaac attcagtttt
      301 tcaacaagcg gttcttcctc tggtgcagct gccacgtgtg taactacggc aggagtgctt
      361 acgggctatt caggattgac cggtggtgca ggttacacct ctgttccaac tattaccatt
      421 actaccggaa ccgagacaac ctatggtgtt tatataggta acaccggaac agtgaggctg
      481 cataacaaca caattagtgc gatacagata gggaatgata ttccggagta ccggcatact
      541 ttttatgcaa ttacctccag tggtacagcc ggtgttcggg aaattaacaa taacaatgtt
      601 ggaagttcaa ctgcaaatgc tattgcggta ggaacaccgt ccacaacctc cggtatttgt
      661 gcattttacg gaattagtaa tagtgctaca ggctcttgcc agatgattga aaatacggtt
      721 aaaaactgta ttgcctatgg tactaccgct tcagcaacta attatggcat gcagaaatca
      781 ggtaattctg cctcattcga catgtccaat aatacagtcg aaaatcttag cagtacggca
      841 aatgcaacca tgataggctt gctgagttcc aatgcttctg tttccggcac tatttccaat
      901 aatgtagtaa ataatctgaa ttccgcaacc acaaccaaca gtgtgagagg aatttcggtg
      961 tcggcagcgg gtaacgtata tgacaacact gtaaccaata ttgtatcggc atcgcttaca
     1021 actggatatt ctgtaggtat gttgttatcg aacggaaccg tagtaaatgt ttatgataat
     1081 acatttaata cagtgcaggg aaatgccttg acaacgggat acgtagcagg gataagtatt
     1141 acgggaggtg ccgccaacga agtgtaccgc aataaaattt atgatatatc ctcatcaagc
     1201 agcagcatgt ctacgggaac agtcaacggt gttgtagttt caggaacaac tacccccatg
     1261 gcttcaatta tatataataa cctgattggg gatgtgaaag ccccatcagc cagctcatca
     1321 actgatgtag ttcgtggtat aagcgttatt aatacgggca ccacttcttc tacaaaatta
     1381 tattacaata ccgttaacct taatgcaagc tcctccggta ctaatttcag ttcatcgggt
     1441 atataccata caactcaaac aacggcaacc agagccagcc ttgaaatgat taacaacctg
     1501 attgtgaacg agtccacccc caagggaaca gggaaagcag tcgcctaccg caggtcttct
     1561 acaacactaa ataattattc tactgcctca aataataact catactacgc cggaaccccg
     1621 ggatccagcc gattgatatt ttatgacggg accaattcag atcaaacaat tgaggctttc
     1681 aaaacgagaa tgagcacacg cgaccaggct tctgtgagta acgatgcagt atttttaagt
     1741 tcaacaggtg ctgatgctac ttttttacat ctcgacaacg acgataactg tcgtattgat
     1801 ggtaaaggaa aagtaatagc aacttataca agcgattatg attcggaccc tcgtgatgca
     1861 tcttatcctg atattggcgc tgatgaggta acaacaggct atcccgccaa tctggccaca
     1921 agcccgacaa cattgcctac taatgcatat gtttggacgg gtcaaacagg aactgacacc
     1981 tgggcaacct cgagcaattg ggtacaatac aatggtagta ctttttcaat accggggagt
     2041 gctcccaatg ctactaatgc aaacgtatat ataagaactt acaatacttg tgttcttgaa
     2101 aatttgccca ccgttacggt gtcttctgac tttaattgcg gaaacctcat aatcgaatca
     2161 agtggtgggt tgacagttac aggtgcaaac aaactgattg tcaatggata tttgaccaat
     2221 gatggtaatt tgacagcctc aggaagtccg acaattcagg tgaatggcaa ttggaccaat
     2281 aacggaacat ttacccctgc aacaagtact gtacttctgg gtggcacagg tgcaactatc
     2341 aataccggag gtgtaggcac tggcaaatct ttctataacc tgaccgttac aaaaaccagt
     2401 ggaactgcaa ccatttctac taataatctt gatgttgatg gaaatctctc aattacttcg
     2461 ggaacacttt cattatcagg ttcgttgtcg gcaacaattg ccggaaactg gtcgcatacc
     2521 ggaggtaatt tcacatgtaa cagctctact gttaccttcg atggaactaa ctctaccatt
     2581 acaaccacca gtaccggtac aaaaagtttt taccatgtta ttattgacga tgcagctgtt
     2641 ttgggtagtg atgttgatat aaatggcaat ttaaccatta acaacgggaa atcattcagt
     2701 gatggttcat tttattcgat gacactggcg gggaactgga ccaataacgg aacatttact
     2761 gctggaagtg tgggaactgt aactttcgat ggaagtgctt cagcaataat cggaggcagt
     2821 tcttccgata cattctttaa aatggctctc aacaaagggt ccggattggg tttaatgctg
     2881 gaagtgaatg taccgattac gttgagtaat acttcgacat ccgccttaac aatgaccaac
     2941 ggattattga aaattaattc tgatggcgaa ttaaatcttc cggctgcagg gcagactatt
     3001 ggtacaggag ctggaattga agttaacggt ggaaccttaa acgggggtag tgcaactatg
     3061 gtgaataatg ggtatttcaa actgacttca ggtacagtaa acattggtgc ggttgcagga
     3121 aattcgttcg aaaacagatc aaactcacgg ttttatatcg aatcaggaat tatgaacatt
     3181 accggaaggc ttatgtttac cgctgcaaat accgatgcca tcattagtgg tggaacacta
     3241 aacctttgta cagttggtaa tacatcatca tcttacgggg ctttcaatat gattggaaca
     3301 gcaaatattt caattactgg caccccactg attactttcc agaatgcagg cagtggtgca
     3361 acgccacttg atttgaatat tctcagtggt acaggtacta aaaacttcag cggaggcacc
     3421 ttgcagtttg ggaatgcttc tactcctgcc gggcagaatt ttaaaatcaa aagcgaggtt
     3481 cccgtgttta atattgtcgt aaatggtaat aataacccaa cggctacatt aacagcgaac
     3541 cttcccggta gcaacaatgt tactatcaat tcggggggaa cactgaatac gtcgtcatac
     3601 cagttaacgc tttcaggtga ctggaccaac aatgggactt atacagctag ttcaggtacg
     3661 gttaattttg caggcacgtc gcagcaagag atacaagcag gaacaggagc atttaataat
     3721 gtcattttca gcaataccac cacag
//
LOCUS       DAOOQP010000188         3700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_29739_length_3700_cov_3.863649, whole genome shotgun sequence.
ACCESSION   DAOOQP010000188 DAOOQP010000000
VERSION     DAOOQP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29739_length_3700_cov_3.863649"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="PLK75_12535"
     CDS             complement(<1..1681)
                     /locus_tag="PLK75_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY40116.1"
                     /translation="MKKYLYLAIMFLAIISCKSGNDFKTIKLTYPETKKTDVTDTYFG
                     VIVADPYRWLENDTADEVENWVKEQNKVTSTYLEQIPFRKQIHQRLTKLWDYPKSSAP
                     TKKGNRYFLYKNNGLQNQNVLYYKNSLQGDDIELLDPNKLSSDGTVSLSSIGVSRDGK
                     YLAYAVSSSGSDWQEIYVRDIEKATDLKDNIKWVKFSGMAWFGDGFFYSRYPEPAKGS
                     EFSGVNEDNKVYYHKIGDSQSADQLVYEDESNSEINFSADVTDDEKYLILYASQSTSG
                     NALYYKDLANTNAPVKKIIDNFDNEYIVIGHYDGMFFVLTNDNAPKYKLISIDPKRSD
                     AKYRKDIIPEEEGVLQGVSVFGDKIIASYLIDAHSVVKVFDIKGKYLYDIDIPIIGTI
                     AGFSGERTDPETFYTVTSFTTPATIYRYDVEKNKSEIYQNPQIDFDATKYETKQIFYT
                     SKDGTKIPMFIVHKKGIALDGSNPTILYGYGGFNISLTPYFSIVRLLWLEQGGVFAMA
                     NLRGGGEYGEEWHKAGTLLNKQNVFDDFIAAAEYLIKQKYTSPTRLAIQGGS"
     gene            1909..3228
                     /locus_tag="PLK75_12540"
     CDS             1909..3228
                     /locus_tag="PLK75_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40117.1"
                     /translation="MKKFILYSLLAFISIALFGQNDDSTHVKTGWNFGLLPVVSYNSD
                     LGFQYGLLTNVYNYGDGKVFPKYYHSIYAEVSRYTKGSGIYRLFYDSEYLIPHIRLTA
                     DISYLPDQALDFYGFNGYNSVYNTRWTNDENISDYKSRMYYKHQRNIFRVKVDFQGKT
                     GIKHLNWAAGYDFLDVKIASVPIDKLNKGKDSTEMLPDVNLLYDDYVDWGIINDAEAD
                     GGMHHSVKVGAVYDTRDNEPCPMKGIWTELVLFNSFSNEFTFGKLAFTHRQYFTIVPK
                     NLSFAYRLGYQGIVYGDAPFYLLPYMIYSYLPSSSIDGLGGSKSVRGLIRNRLVADGV
                     VYGNVEFRWKFVRFNFIKQQWYLALNPFFDFGRTVQNRLIDVRNIPPTVNQSIYFDYG
                     AESFHFAYGAGLHIAMNENFVIAAEMGMPVDKRDGKMGIYIGMNWLF"
     gene            complement(3280..>3700)
                     /locus_tag="PLK75_12545"
     CDS             complement(3280..>3700)
                     /locus_tag="PLK75_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY40118.1"
                     /translation="LLFVFACGNKNTENKSSGSSSPSTNQQNETTGQEVYTFENVDEQ
                     PVFGDTEDALMMFVAKNTKYPPEAKENGIQGRVFVSFVIDEKGKVTDAAIQKGANEML
                     DAEALRVVNSLPAWKPAQKGGKAVKVSFVLPINFKLQ"
BASE COUNT         1029 a          767 c          716 g         1188 t
ORIGIN      
        1 tcgagcctcc ctgtatcgct aaccgggtgg gcgatgtgta cttttgcttg ataaggtatt
       61 cggctgctgc tataaaatcg tcgaaaacat tttgtttgtt cagtagggta cccgctttat
      121 gccactcttc gccatattct cctcctccac gcaaatttgc cattgcaaac actcctccct
      181 gttcaagcca caacagccga actatgctga aataaggtgt caaactgata ttaaaacctc
      241 cataaccgta cagaattgtc gggttacttc catcaagggc aatacctttt ttatgtacaa
      301 tgaacatagg tatttttgta ccgtcctttg atgtgtaaaa aatttgtttg gtttcgtatt
      361 ttgtagcatc gaaatcaatt tgtgggtttt gataaatttc cgacttgttt ttctccacat
      421 cgtagcggta tatggtggca ggtgtagtaa acgatgtaac cgtatagaaa gtttcaggat
      481 ctgttctttc accggaaaaa ccggcaattg tgccaattat tgggatatca atatcgtaca
      541 gatatttccc cttgatgtcg aaaactttta caactgaatg ggcgtcaatc agataagatg
      601 caataatttt atctccaaac acactcacgc cctgcagaac gccttcctct tcaggaatta
      661 tatcctttct gtatttagcg tctgaccttt tcgggtcaat ggatatgagt ttgtactttg
      721 gagcattatc attggtaagt acaaaaaaca tcccgtcata atgccctatt acaatgtatt
      781 cattatcgaa attatcgata attttcttaa caggtgcatt tgtattggca agatctttat
      841 aatacaatgc attgcccgaa gttgactgac ttgcataaag tatcaggtat ttctcatcat
      901 cggttacatc agccgaaaaa tttatttcag aattcgattc atcttcataa acgagctgat
      961 ccgccgactg tgaatcacct attttatgat aatacacctt attgtcttca ttaaccccac
     1021 taaactctga cccttttgcc ggttcaggat aacggctgta gaaaaaacca tcgccaaacc
     1081 atgccatgcc tgaaaacttc acccatttga tattgtcttt cagatcggtc gctttttcaa
     1141 tgtctctgac gtaaatttcc tgccagtcgg aaccgctgct cgaaactgca taagccagat
     1201 atttcccgtc acgtgaaaca cctatcgacg acaacgacac agttccatcg gatgaaagct
     1261 tgttcgggtc aagcagttca atatcatcac cttgcaggct gtttttatag taaagcacat
     1321 tctggttctg aagtccgttg tttttataca gaaaataccg attacctttt ttggttggag
     1381 ccgaagattt cgggtaatcc cataattttg taagacgttg gtgtatctgc tttctgaatg
     1441 gtatttgttc caaataagtt gaggtaacct tattctgctc ttttacccag ttctccactt
     1501 catccgcagt gtcgttttcg agccagcggt atgggtcagc aacaataacc ccgaaataag
     1561 tatcagtaac atcagttttt tttgtctcag gataggtaag cttaattgtt ttaaaatcgt
     1621 tgccactctt acacgaaatt attgctaaga acataattgc caggtataaa tattttttca
     1681 tagctctttt ttttgtaaaa atagcaatat tcaacagtgc tgcttctatg cttaacacat
     1741 ctttaacaaa atagcaactt ttaaaggcaa acgcattcaa tgcaatctaa ttatcatcag
     1801 tagccttaat tacatcgaca aatatgctga atgttaaaat tattgatgaa tttgcatgtg
     1861 tgttatctga aatttaacaa atacgttaaa tttgcccaaa tttgaattat gaaaaaattt
     1921 attctttatt cactgttggc ttttataagc attgcattgt ttggtcagaa cgatgactcc
     1981 acacatgtaa aaacaggctg gaactttggg ttgctgccgg tggtttctta taactctgat
     2041 ctcggatttc agtacggatt gctcaccaat gtgtataatt acggtgatgg taaggttttt
     2101 ccgaaatatt atcactctat ctatgctgaa gttagccgct atacaaaagg atcgggtata
     2161 taccgccttt tttatgattc ggaatatctg atcccgcaca tcaggctcac agcagacata
     2221 tcatatctgc ctgatcaggc ccttgacttt tacgggttca acggatataa ttcagtgtac
     2281 aacacccgct ggacaaatga tgaaaatatt tctgattaca agtcgcgcat gtactacaag
     2341 catcagagga atattttcag ggttaaagta gattttcagg gtaaaacagg tattaagcat
     2401 ctcaactggg ctgccgggta cgattttctt gatgttaaaa ttgcctctgt acctatcgat
     2461 aagctcaata aagggaaaga cagtactgag atgttacctg atgttaattt gctgtacgac
     2521 gactatgtag attggggaat tattaatgat gctgaagcag acgggggaat gcatcacagc
     2581 gtcaaagtcg gagcggtgta cgatacccgc gacaacgaac cctgcccaat gaagggaata
     2641 tggacagaat tggtactttt caacagtttc agcaatgaat tcaccttcgg gaaactggca
     2701 tttacccaca gacaatactt cacaattgta cccaaaaatc tatcgtttgc ttaccgtttg
     2761 ggatatcagg ggattgtgta tggtgatgcc ccgttttatc tgctgcctta tatgatatac
     2821 tcttacctgc cgtcttcatc tatcgacgga ctgggtggta gcaaatcggt gcgcggtctc
     2881 ataagaaacc gtctcgtagc cgacggagtt gtgtatggaa atgtggaatt tcgatggaaa
     2941 tttgtgcggt ttaattttat caaacaacaa tggtaccttg ccttaaatcc tttcttcgac
     3001 tttggacgca ccgttcaaaa caggttgatt gatgtgcgta atattcctcc tacggtgaat
     3061 cagagcatct attttgatta tggggctgaa agttttcatt tcgcttatgg agcagggctt
     3121 catatagcca tgaacgaaaa ttttgtaatc gctgctgaaa tgggtatgcc ggttgacaaa
     3181 cgcgatggaa aaatgggcat ttatattggt atgaactggc tgttttagtg ataggctcac
     3241 cttatttccc agattatcag gggaagatac atctgatttt tactgtaatt taaagttaat
     3301 aggcaaaaca aagctaactt tcactgcttt tccacccttt tgagcgggct tccatgccgg
     3361 taagctattc acaacacgca aagcctcagc atcaagcatt tcgttagcac ccttctggat
     3421 tgcagcatcg gtaaccttac ccttctcatc aattacaaaa cttacaaaca ctctcccctg
     3481 tatgccattt tcttttgctt cgggagggta ttttgtattt tttgcgacaa acatcatcag
     3541 cgcatcctct gtgtcgccaa ataccggttg ctcatccacg ttttcgaaag tatatacctc
     3601 ctgccctgtg gtttcatttt gttgattagt tgacggagac gaactaccgg aagatttgtt
     3661 ttcggtgttc ttgttgccgc atgcaaagac aaacagaagc
//
LOCUS       DAOOQP010000189         3699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_29746_length_3699_cov_6.344676, whole genome shotgun sequence.
ACCESSION   DAOOQP010000189 DAOOQP010000000
VERSION     DAOOQP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29746_length_3699_cov_6.344676"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..171
                     /locus_tag="PLK75_12550"
     CDS             40..171
                     /locus_tag="PLK75_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOY40119.1"
                     /translation="MSKSGVKLLGACGCCPHSIQTLKLGVEAKLMEDVPEVKEVVAV"
     gene            complement(210..1640)
                     /locus_tag="PLK75_12555"
     CDS             complement(210..1640)
                     /locus_tag="PLK75_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOY40120.1"
                     /translation="MKLKVFVLFAIFLGSIAFPATAQQNSQNKKTAKYVFLFIGDGMG
                     LAHISLTQAYLANKSGEIGMGALCMTEFPVVGLCNTSCSNKQTTCSAASGTALSTGSK
                     TGFGIIGKTADKTKNLSSITYSIKNAGCRVGLITTVSINHATPAAFYAHSDKRSDYYS
                     IGEQLCESNFDFFAGGGFYFNKGRDGLGADLYDLATRQGYTIIRDSVDTKHFFSKSKT
                     ILLNPVSLLESEMPLAIDRDEYGGYNLSNLVEAGIQTLTNDQGFFIMVEGGLIDWAAH
                     YKDAAAIVQEVLDFDKSIGVALDFYRKHPDETLIVVTADHETGGLSLGRSEFEYETDF
                     ARIDEQKNSASFVAPMLDTLIKNTASFTEVQNLLLQEFFADGFILSDAEIFELNYAYE
                     YACGLFSLDVESERMLYGGNQPVAAAAAQIINKRSGIGFTSYYHTGISVPVFAIGTGS
                     ELFGTYLDNTDIPMIILDCMRIKKIE"
     gene            1740..3206
                     /gene="cysS"
                     /locus_tag="PLK75_12560"
     CDS             1740..3206
                     /gene="cysS"
                     /locus_tag="PLK75_12560"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOY40121.1"
                     /translation="MEHKLYIYNTLTREKELFIPISEPYVGMYVCGPTVYGDAHLGHA
                     RPAINFDLLFRYLRYLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEALEPMEIV
                     QYYTDRYHRNMDALNVLRPSIEPRASGHIIEQIALTQRILDSGYAYVSNGSVYFDVEK
                     YNATYNYGKLSGRVLEELYSTTRDLEGQSEKRKSYDFALWKKASPEHIMHWPSPWSEG
                     FPGWHMECSAMGMKYLGEVFDIHGGGMDLMFPHHECEIAQSAAAMGKETVRYWMHNNM
                     ITINGQKMGKSLGNFITLFDIFTGNNSLLEQAYSPSTVRFFILMAHYRGTLDFSNEAL
                     QAAEKGLLRLMKGFTAIDKIKPSQKTSFSISEFEQKLQNALNDDLNSPVLIAHLFDGL
                     KTVNNLIVGTDTITEQDLIAYKNVYSTFLFDILGLELPTENNQQILGKVVDLLLNLRV
                     EAKNNKNYALSDQIRNELNKLGIVVRDSKDGFEWEISG"
     gene            complement(3236..>3699)
                     /locus_tag="PLK75_12565"
     CDS             complement(3236..>3699)
                     /locus_tag="PLK75_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY40122.1"
                     /translation="GEMIKNVWFSKSEYNESPFKFEAGTPDYVGAAALGSALDYIADI
                     GIDSIAKHEKKLLEYATDKLRNIDGFRIIGNASNKCSVISFLIGNTHPYDVGVLLDQL
                     GIAVRTGTHCAETVMQHYNISGTARISFGLYNSPEEIDIMTNSLARIVKML"
BASE COUNT         1115 a          726 c          790 g         1068 t
ORIGIN      
        1 tttgaagaaa acttttggga gaatttttag ggtggcgcat tgtcgaagtc aggagttaag
       61 ctgctcggcg cctgtggatg ttgtccgcat agtattcaga cccttaagct gggtgttgaa
      121 gccaaattga tggaagatgt acccgaagta aaagaagttg tggcagtata ggatactgtt
      181 caaattctta atgccgaacg aatcgatggc tattctattt ttttaatcct catacaatcg
      241 aggattatca tcgggatatc tgtgttgtcg aggtatgtcc caaacaattc actgcccgtt
      301 ccaattgcaa atacaggaac ggaaataccc gtatgataat acgacgtaaa acctataccg
      361 gatcttttgt ttattatttg cgctgctgca gctgcaacgg gctgattacc accatataac
      421 attcgttccg attcaacatc aagcgaaaac aaaccacagg catattcata cgcgtaattc
      481 aattcaaata tttcagcatc agaaaggatg aatccatctg cgaaaaactc ctgtaaaagt
      541 aagttttgca cctcggtgaa tgaggcagtg ttcttaatta atgtatctaa cataggggcg
      601 acaaaagaag ctgaattctt ttgttcatca atccttgcaa aatcggtttc gtattcgaat
      661 tcgctcctgc caagtgataa tccaccggtt tcatggtcgg cggttacaac aataagtgtt
      721 tcatccgggt gttttcggta gaagtcgaga gccacaccaa tagacttgtc aaaatctaac
      781 acttcctgta caatagcagc agcgtcttta taatgtgcgg cccagtcgat aagaccccct
      841 tcaaccataa taaaaaatcc ctggtcattg gttaatgttt gaatacccgc ttcaacaaga
      901 ttactcaggt tatagccacc atattcatca cgatctattg ccaatggcat ttccgattcc
      961 agcaggctga caggattcag taaaatagtt ttactcttcg aaaagaagtg ctttgtgtca
     1021 accgaatcac ggataatggt atatccctgg cgggttgcca aatcatataa gtccgcacca
     1081 agtccgtcac gacctttatt gaaataaaag cctccacctg caaaaaaatc gaagttagat
     1141 tcgcacagtt gttctcctat actgtaataa tcagaccttt tatcggaatg tgcataaaaa
     1201 gccgcggggg ttgcgtggtt aatacttacg gttgtgatta atccaaccct acaccctgca
     1261 tttttaattg agtaagtaat actggagagg ttttttgttt tatctgcagt ttttccaata
     1321 atcccaaatc cggttttcga acctgtcgac aaggctgtcc ccgaagctgc actgcatgtt
     1381 gtttgtttgt tgctgcacga tgtattgcac aagcctacga ccggaaattc ggtcatgcac
     1441 aaagcaccca taccgatttc gcccgacttg tttgccaagt aagcttgggt tagcgaaatg
     1501 tgcgcaagcc ccatgccgtc tccaatgaat aagaatacat atttggcagt ttttttattc
     1561 tgtgaattct gttgagccgt ggcaggaaaa gcgatgcttc ccagaaaaat ggcaaatagt
     1621 acaaatactt ttaatttcat aaaggataaa ttgagataat tccaatgcaa aaatagcagg
     1681 atttgtccga agagtattac atttgggaaa tcttaacaat aaaaaatatt ttgtgtctta
     1741 tggaacataa actgtacata tacaatactt tgacacggga gaaagagttg ttcattccta
     1801 ttagcgaacc ttatgtgggt atgtacgttt gcgggccaac tgtgtatggc gatgcgcatc
     1861 tcggacatgc cagaccagcc ataaactttg atctgttgtt tcgctatctg cggtatttgg
     1921 gctataaagt gcggtatgta cgaaacatta ccgatgtggg gcacttagaa aatgatgccg
     1981 atgagggaga ggataaaatt gcaaagaaag cacggctcga agcccttgaa cccatggaaa
     2041 tagtgcagta ctacaccgac aggtatcata ggaatatgga cgctctgaat gtacttcggc
     2101 caagcattga accacgcgct tccggacata taattgaaca aattgcttta acacaacgaa
     2161 ttcttgatag tggctatgct tatgtgtcga atggttctgt gtattttgac gtagagaaat
     2221 acaatgcaac ttataattat ggaaaactct cgggcagggt tcttgaagaa ttatacagta
     2281 caacacgtga tcttgagggg cagagcgaaa aaagaaaaag ctacgatttt gctttgtgga
     2341 aaaaagcttc gccagaacat ataatgcact ggccttctcc atggagtgag ggttttccag
     2401 gttggcacat ggaatgctca gcaatgggaa tgaagtatct gggagaagtt ttcgatattc
     2461 atggaggagg catggattta atgttcccgc atcatgagtg tgagattgct cagagtgctg
     2521 cggctatggg caaggaaaca gtaagatatt ggatgcacaa caatatgatt actataaatg
     2581 ggcaaaaaat gggaaagtcg ctgggtaatt ttattacctt attcgatatt tttacaggga
     2641 acaattcctt actcgaacag gcgtatagtc cttcaacggt gcgatttttc attctaatgg
     2701 cacactacag ggggacactc gacttttcga atgaagccct acaggctgcc gaaaaaggat
     2761 tgctccgact tatgaaaggt tttacagcta tcgataaaat taagccatca cagaaaacaa
     2821 gctttagtat aagcgaattt gagcagaaac tacagaatgc attgaacgac gatttgaata
     2881 gccctgtact cattgctcat ctattcgacg gattaaaaac tgtgaataat ctcattgttg
     2941 gaacggatac tattacagaa caggacctga ttgcttacaa aaatgtttat tccacatttt
     3001 tgttcgatat acttggactg gaattgccaa cggaaaacaa tcagcaaata ctgggtaagg
     3061 ttgttgattt attgctgaac cttcgcgttg aagcgaaaaa caacaaaaac tatgctcttt
     3121 ccgatcaaat cagaaacgaa ctgaataaat tgggcattgt tgttcgcgac agcaaagacg
     3181 ggtttgaatg ggagatttcg ggttagtgta atttgttcag atgaagtgga tgctattaaa
     3241 gcatttttac aatacgggct aaactatttg tcattatatc aatttcctcc gggctattat
     3301 atagaccgaa cgatatgcgt gcagttccac taatgttata atgttgcatc acggtttcgg
     3361 cgcagtgtgt gccggttctt acagctatac ccagttgatc aagcaaaacc ccaacatcat
     3421 atgggtgagt gtttccaata aggaaagaaa taactgagca tttatttgag gcattaccga
     3481 taattctaaa tccatctatg ttccggagtt tatcagtagc atattcgagc aacttttttt
     3541 cgtgtttagc tatagagtcg atgccgatgt cagcaatata atcaagtgcc gaccccaaag
     3601 ctgcagcacc tacgtaatcg ggagttcccg cttcaaattt aaagggactt tcgttgtact
     3661 cgcttttcga aaaccatacg ttttttatca tttctccac
//
LOCUS       DAOOQP010000190         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_29989_length_3676_cov_3.976526, whole genome shotgun sequence.
ACCESSION   DAOOQP010000190 DAOOQP010000000
VERSION     DAOOQP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29989_length_3676_cov_3.976526"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLK75_12570"
     CDS             complement(<1..488)
                     /locus_tag="PLK75_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40123.1"
                     /translation="MLRIYVIIVGLLMIDLTLFSQSEKRYSTNRKHLSLKAGEREYYA
                     KNLPDVVDNSEEIYFPPIYTQTHWVCNQSAASYYMYTYEHNRVKNIASTDEDAWFSVY
                     YPWNWFHGGYGWNGGHVTGTFNIGKNMGLPYLYQSPADLSRDSSIWVSGYDNYLSFMQ
                     NR"
     gene            565..2583
                     /gene="uvrB"
                     /locus_tag="PLK75_12575"
     CDS             565..2583
                     /gene="uvrB"
                     /locus_tag="PLK75_12575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOY40124.1"
                     /translation="MEFILVSDYEPTGDQPQAIRELTDGVFEGRRHQVLLGVTGSGKT
                     YTIANVIQNIQRPVLVLSHNKTLAAQLYSEFKNFFPNNAVEYFVSYYDYYQPEAYLPV
                     SDTYIEKDLAINDEIEKLRLSATSALLSGRRDVIVVSSVSCIYGIGNPDDFYANTIRF
                     SKGDVIGRDQLLRNLVDSLYFRSEIDFVRGSFRVRGDTVDIYLAYQDMALKIEFFDDE
                     VENIFSFDPVHGVIIEKFEQAIIFPANIFVTAKSRMQEAMIQIQDDLGLQVEYLKSIG
                     KRYEAKRLLERVTYDLEMILELGYCSGIENYSRYFDGRATGTRPFCLLDYFPGDYLMV
                     VDESHVTIPQVRGMYGGDRSRKENLVSYGFRLPAALDNRPLKFDEFEQLQNQVIYVSA
                     TPHDYELEKAEGIITEQLIRPTGLLDPEIHVRSSQNQIDDLLNEIRLSTANNERVLVT
                     TLTKRMAEELTKYLVKYQIRCRYIHSDVDTLDRVVILDELRKGLFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNARSLTQTAGRAARNVNGKVIMYASKMTDAMKRTINET
                     NRRRSKQIQYNTDNQIVPRSIVKTTETALSLSSRTEGSPADFVNAESDPGIAADPVVK
                     YMDKPALEKLLKNTKKQMEDAAAKLDFIEAARLRDELYILQKMISSKS"
     gene            complement(2707..>3676)
                     /locus_tag="PLK75_12580"
     CDS             complement(2707..>3676)
                     /locus_tag="PLK75_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY40125.1"
                     /translation="FTEVNWGCMGYETGANETLFGTGRANTELIFNVCPDQTAAANLI
                     YSQEYYGYFDWALPSRDELVEMYTKKEYIPGFGDFSYWSSSEDVTDPMNSAWCMIIGE
                     TSLSSKSITYNARPIRTFGLEDVYDYEGNSYGVVVIGTQVWMAENLRSTYFQNGEWIT
                     LTTDDTEWYSLIAGSPSYCYYDNMIDYLYSLGGLYNFYAAADSRNICPYGYHVPTDAE
                     WTTLVTYLGGEGLAGGALKVTGADYWGEPNIGATNSTNFNALGAGMRNGTTGLYDGML
                     MNTGYWTSTPTDVSSSWLRTLSFNNAAATRTSNDNHTGASVRCIRD"
BASE COUNT         1096 a          759 c          785 g         1036 t
ORIGIN      
        1 attctgtttt gcataaaact caggtaattg tcgtatcccg acacccaaat ggaagaatct
       61 ctcgataaat cggcaggcga ttgatataaa tatggcaatc ccatgttttt gccaatatta
      121 aaagtgccgg ttacatgtcc gccattccag ccatatcctc catgaaacca attccatggg
      181 taatacacac tgaaccatgc gtcctcatca gttgaggcaa tatttttaac ccggttatgc
      241 tcataggtgt acatataata cgatgctgcc gactggttgc atacccagtg ggtctgcgtg
      301 taaatgggag ggaaatatat ctcttcggaa ttatccacaa catcgggcag gttctttgca
      361 taatattctc tttcgcctgc tttcagtgat aaatgtttgc ggttggtgct ataccttttt
      421 tcggactgag agaacagggt caggtcaatc atcaacaacc caacaattat gacataaata
      481 cgaagcatta ataaatgata atgacatttc ttgcgaaaat aatatattat tgatttattt
      541 ttgtgaatta ttatatattg ttcaatggaa tttattctgg tatcggatta tgaaccaaca
      601 ggagatcaac ctcaggccat acgtgaactt actgatggtg tttttgaagg gcgtagacat
      661 caggtgttgc tcggagttac cggtagtggg aaaacctata caatcgctaa tgtaatacag
      721 aatattcagc gtccggtact ggtattgagc cataataaaa cacttgctgc ccagctttac
      781 tctgaattta aaaacttctt ccccaataat gcggttgaat attttgtttc ttattacgat
      841 tattatcaac ctgaagcgta tttgccggtt tctgatacct atatcgagaa agaccttgct
      901 attaatgatg aaattgaaaa actgcgcctc agtgccacca gtgcactgtt gtcgggcaga
      961 agagatgtga ttgttgtttc ttcggtatcg tgtatttacg gaattggcaa ccccgatgat
     1021 ttttatgcga ataccattcg ttttagcaaa ggagatgtta ttggcagaga ccagctgcta
     1081 cgaaatctgg ttgatagcct gtatttccgc tccgaaattg attttgtgcg tggaagcttt
     1141 cgggtaaggg gcgatacagt cgatatttat ctggcctatc aggatatggc tttgaaaata
     1201 gagttcttcg atgatgaagt ggagaatatt tttagcttcg accctgttca tggtgtaatt
     1261 attgagaagt ttgaacaagc aataattttt ccggcaaata tttttgtcac ggcaaagtcg
     1321 aggatgcagg aagccatgat acagatacag gatgaccttg gtctgcaggt cgaatattta
     1381 aaatccatag gtaagcgata cgaagcaaaa agattgctcg aaagggttac ttatgacctc
     1441 gaaatgatac tggaactggg ttattgcagt ggtatagaaa actattcgag gtactttgat
     1501 ggaagagcta cggggacccg tccgttttgc ctgcttgact atttccccgg agattatctg
     1561 atggtggtcg acgaaagtca tgttaccata ccacaagtgc gtggtatgta tgggggcgac
     1621 aggtcgcgaa aagaaaacct tgtttcgtat ggcttcaggt tgccggcagc cctcgataac
     1681 cgcccattga aattcgatga attcgagcag ctgcagaatc aggtaattta tgtaagcgca
     1741 acacctcacg actatgaact ggaaaaagcc gaaggtatta ttaccgaaca gctcatcagg
     1801 ccaacaggtt tactcgatcc tgaaatccat gtcagatcaa gccagaacca aattgatgat
     1861 ttgctgaacg aaatacgctt aagtacggca aacaacgaaa gggtgctggt aaccaccctg
     1921 actaaaagaa tggcagaaga actgaccaaa tacctggtaa agtatcagat tcggtgcaga
     1981 tatattcatt ccgatgtgga tacactcgat agggttgtca tcttagatga acttcgtaaa
     2041 ggtctattcg atgtgctggt tggagtgaat ctgttgcgcg aaggtctcga tctccccgaa
     2101 gtgtcgttgg tggcaatact tgatgccgat aaggaaggtt ttctcaggaa tgcacgttcg
     2161 ctcacgcaga cagccggccg tgccgcacgt aatgtaaacg gaaaggttat tatgtatgcg
     2221 agcaaaatga ctgatgccat gaaaagaacc atcaatgaga cgaatcgccg ccgaagtaag
     2281 caaatacaat acaataccga taatcagatt gttccgcgca gtattgtaaa aactactgaa
     2341 acagcattgt cgctatcttc aagaacggaa ggttctcctg ccgattttgt taatgcagaa
     2401 tcagacccgg gaatagctgc cgatccggtt gtgaaatata tggataaacc tgcattggaa
     2461 aaattgctca agaataccaa aaagcaaatg gaagatgctg cagccaaact cgattttatc
     2521 gaagcagcca gactcagaga cgaattatat attttgcaga aaatgattag ctcaaaaagt
     2581 taaaaaaact cgttccagca ggtgtagctt attaatctgc agctccctca tctttgatgc
     2641 aacgtacaga aaaacctttt gacatttcaa tgctaacatt gcttatgtta aacccaaaat
     2701 cagcaatcaa tcccggatac aacgaaccga tgcgccggta tgattatcgt tacttgtacg
     2761 tgttgcagca gcattgttaa aactaagggt cctcagccaa cttgaagata catcagttgg
     2821 ggtgctggtc cagtaacctg tattcatcaa catgccatcg tacaatccgg ttgttccgtt
     2881 acgcatacct gctcctaatg cattaaaatt cgttgaattt gtagcaccaa tattaggctc
     2941 tccccagtag tcggcaccgg taactttcaa agcacctcct gccaatcctt caccgcctaa
     3001 ataagtaacg agtgtagtcc attctgcatc ggtaggcaca tggtagccgt atggacagat
     3061 attgcgtgaa tcagctgcag cataaaaatt atataatccc ccaaggctgt agagataatc
     3121 aatcatatta tcgtaatagc aatatgaggg cgatccagcg attaacgaat accattctgt
     3181 gtcatcggtt gtgagggtaa tccactcacc attctgaaag taggtgcttc ttaaattttc
     3241 agccatccat acctgtgttc ctataaccac aaccccatat gagttgcctt cataatcgta
     3301 aacatcttca agtccgaaag ttctgatggg tctggcatta taggtaattg atttggagga
     3361 aaggctggtc tctccaataa tcatacacca tgcactattc attggatccg ttacatcttc
     3421 agaagatgac caataggaga aatcaccaaa ccccggaata tattcttttt ttgtgtacat
     3481 ttcaacaagt tcatctctcg atggaagtgc ccaatcgaaa tatccataat actcctgaga
     3541 ataaatcaaa ttggcagcgg ccgtctgatc agggcaaaca ttaaaaatta attccgtgtt
     3601 tgcgcgacct gttccaaaca gagtttcatt ggcaccggtt tcgtatccca tgcatcccca
     3661 gtttacctca gtaaag
//
LOCUS       DAOOQP010000191         3658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_30168_length_3658_cov_8.341937, whole genome shotgun sequence.
ACCESSION   DAOOQP010000191 DAOOQP010000000
VERSION     DAOOQP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30168_length_3658_cov_8.341937"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..799
                     /locus_tag="PLK75_12585"
     CDS             92..799
                     /locus_tag="PLK75_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40126.1"
                     /translation="MRIISWNCQGAFRNKTEPALQCKPDILVIQECENAEKIVFKDIH
                     PTDFFWYSDDGKKGLGLFSFSDYRFRLMDEFDPRFRYILPIEVLGTNFNFTLMAIWAM
                     NNKENRRARYIGQVWLAIQHYEKLLEGNVILMGDFNSNKIWDYKSRVGDHSDVVNKLE
                     ALNISSLYHLYYNQQQGEEKHQTLYMNRNIERSYHVDYCFASSYFKAKLKSLQIGDFN
                     EWLKYSDHCPLIVDFNS"
     gene            806..1648
                     /locus_tag="PLK75_12590"
     CDS             806..1648
                     /locus_tag="PLK75_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40127.1"
                     /translation="MFENYFFGNVYLYCLPEHPMKKEASSFKYLSLRKTNKNVKFSTG
                     ELVLLLYRKSSNIAPGIIGFCKVAGDVLTTKEIPKENRKYCNNQSLKQLPVSDIQCYG
                     GQNLIDEEMLYALPQMNRYGKYRVFAECSLISVPGSALSALNDYSKLWYIWRNPVSPE
                     RQHGFKQLFIHMQNEKIVFKKYHAMIHKVTQCAICGVEHKAFEKYSLPFFELHDAMVH
                     AYGKYEKPKYENYIPLCANCHNNEHEKIVMEKAVNSISNQSDGSSARLLTSWDIEYFK
                     EILK"
     gene            complement(1671..2174)
                     /locus_tag="PLK75_12595"
     CDS             complement(1671..2174)
                     /locus_tag="PLK75_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOY40128.1"
                     /translation="MNRTDLYIDLEWFFNQQIFLVGYAYSVTNYNQLYDENLNMYNIQ
                     RMLAPVDGYIYFYGPDIGMLEKNTGLDIRNNYRCVNLLKVFRDIMPGMDSYKLAHFEE
                     MFEINRSQRQYKSNIFKLAEDWNNPYKKQHVLKYNLEDVVNLVRLKKEIFSIYGIGEE
                     YLEGVRW"
     gene            2325..2510
                     /locus_tag="PLK75_12600"
     CDS             2325..2510
                     /locus_tag="PLK75_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40129.1"
                     /translation="MQEKKSYTPKELADEYNVCLRTFFTWISPIRQEIMAMNPNHKKR
                     ARVFIPKQVKRIREFLG"
     gene            2613..3173
                     /locus_tag="PLK75_12605"
     CDS             2613..3173
                     /locus_tag="PLK75_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40130.1"
                     /translation="MSKLKKIIIVVAVLLTLGIAWKLFRKVKDTDKLKIGDTSLKPTK
                     EIKKFTDLAEIFSSGLKVKGFIEIRNFSGRDYTLSQLNIDCFTPKTETLLAEQTNIIE
                     KNIVLKAKQTTNIPLEYTVNIVNALLMFKESEVLPADATVWQVISQPAQYWKEINMKK
                     LTMKLKGFIVAEGINLSINMDYPLYD"
     gene            3166..>3658
                     /locus_tag="PLK75_12610"
     CDS             3166..>3658
                     /locus_tag="PLK75_12610"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOY40131.1"
                     /translation="MTETYAITGKSTTLKQYADVHDTIAAIKRIVKKNAPVLYGLSAD
                     IEGRNAVATFRNIWDYVRENIRYKNDEPGKEQLRTPQRTLMDRTGDCDDMTILISALL
                     TNMGYDHELCIAAYKRPDQWQHIYPAVYNEYGNRYVIDCVPEIPYFNYEAQPIKNQII
                     VPMK"
BASE COUNT         1175 a          720 c          759 g         1004 t
ORIGIN      
        1 aggaagcgaa agtttccttt tttaatatca catattatcc tagttggtta attcttaaat
       61 aatcttttac ttttgataaa aattgccaat aatgcgaata atcagctgga attgccaggg
      121 tgcttttcgg aataagactg aacctgcgtt gcagtgcaaa cctgatatac ttgtgattca
      181 ggaatgtgaa aatgctgaga aaatagtttt caaagatatt catccaactg attttttctg
      241 gtatagcgat gatggaaaaa agggacttgg tttattctcg ttttcggatt atcgttttag
      301 gcttatggat gaatttgatc ctaggttcag gtatatatta ccaatagaag tgttaggtac
      361 gaatttcaat tttactctga tggcaatatg ggcaatgaac aataaagaaa atcgcagggc
      421 acgttatatc ggacaagttt ggttagcaat tcagcattac gaaaaacttt tagaaggaaa
      481 cgtgattctg atgggtgact tcaacagcaa taaaatatgg gattacaaaa gtcgcgttgg
      541 tgaccattct gatgtagtaa ataaactgga agcattgaat atttcaagct tgtatcatct
      601 ctattataat cagcaacagg gagaagaaaa acatcagaca ctttacatga accgaaatat
      661 tgaaaggtct taccatgttg actattgctt tgcatcatcg tattttaagg caaaactcaa
      721 gtccttacaa ataggtgatt tcaatgagtg gttaaaatat agtgatcact gcccgcttat
      781 tgttgatttt aattcgtagt ccgtaatgtt tgaaaattac ttttttggaa atgtttattt
      841 gtattgtttg ccagagcatc caatgaaaaa ggaagcgtca tcattcaaat atctatcatt
      901 gcgtaagaca aataagaatg tgaagttttc aactggagag ctggttctac tactgtatcg
      961 aaaaagttcg aatatagccc cgggtataat cggattttgc aaagtggcag gggatgtatt
     1021 aactactaaa gaaataccta aagagaatcg taagtattgc aataatcaat ctctaaaaca
     1081 attaccggta agcgatattc aatgttatgg cggtcagaat ctaattgatg aagaaatgtt
     1141 gtatgcactt cctcaaatga accgatatgg taagtatcgt gtatttgcag aatgcagttt
     1201 aattagcgta cctggttctg cattatcagc tttgaacgat tatagcaaat tgtggtatat
     1261 ctggcggaat ccagtgtctc cagaaaggca acatggattt aaacagctat ttatacacat
     1321 gcagaatgaa aaaattgtgt ttaaaaaata ccatgcaatg attcacaaag ttacccaatg
     1381 tgcaatttgt ggtgttgaac acaaagcatt tgagaaatat agcctgccat ttttcgaatt
     1441 acatgatgcg atggtacatg catacgggaa atatgaaaaa ccaaaatatg agaattacat
     1501 tcctctttgt gcaaattgcc acaacaatga acatgaaaaa attgtaatgg aaaaggcagt
     1561 aaattcaatt tcaaatcaat ctgatggtag ttcagctcgt ttattaacaa gttgggacat
     1621 tgaatatttt aaagaaatct tgaaataatt gtacttggct taagtgagat tcaccacctc
     1681 accccctcca aatactcttc cccaatccca tagattgaga atatttcctt cttcagccga
     1741 actaggttta ccacgtcttc gaggttgtat ttgaggacgt gttgtttttt gtaggggttg
     1801 ttccagtctt cggcaagttt gaagatgttt gatttgtatt ggcgttgcga acgattgatt
     1861 tcaaacattt cctcgaaatg ggctagtttg tacgagtcca tcccgggcat gatgtcacgg
     1921 aatactttca gcaagttcac acaacggtaa ttgttgcgga tatccagacc ggtgttcttt
     1981 tcaagcatcc cgatatcggg accgtaaaag taaatgtagc cgtccactgg tgccagcatg
     2041 cgttggatat tgtacatatt caggttctca tcatagagct ggttgtagtt ggtcaccgag
     2101 taggcatatc ccaccaggaa gatctgttgg ttgaaaaacc attcgaggtc gatgtagagg
     2161 tcagttcggt tcatagcaaa taataaatgt ttgaatgcaa attattttca ggaggtaaag
     2221 ctacatcaag attttcggaa gtggctgttc tccggtgcgt ttgcagcgaa atgtgtcgtt
     2281 ttgcgtagtt gatattcaga cgcttacatg tatctttgta gcgtatgcaa gagaaaaagt
     2341 catatacccc caaagagctg gccgacgaat acaatgtttg cttgcgtacc tttttcacat
     2401 ggatatcacc catacgccag gagatcatgg ccatgaaccc caaccacaaa aaaagagcaa
     2461 gggtatttat cccgaaacag gtaaaacgca tccgcgagtt tctgggttaa cggcaaacaa
     2521 cggcaaacaa taaccgttaa taccgataac acagttaacg gcaagcagag tttttcgaca
     2581 ccctaatatt gtgatgaaat ttcatcacga tcatgtcgaa actgaagaaa ataatcattg
     2641 ttgttgcggt cctcctgacg ctgggcatcg catggaaact cttcagaaaa gtaaaagaca
     2701 ccgacaaact caagattggc gacacaagcc tgaaacccac caaagaaatc aaaaagttca
     2761 ccgatctggc tgagatattc agttccggac tcaaggtaaa aggcttcatc gagataagaa
     2821 atttctccgg cagggactac accctgagtc aactgaacat cgactgtttc accccgaaaa
     2881 ccgaaacact gctggctgag caaaccaaca tcatcgaaaa aaacattgtg ctcaaagcca
     2941 aacaaaccac caatatcccg ctggaataca ccgtgaacat tgtgaacgca ttgctcatgt
     3001 tcaaagaaag cgaagtgtta cctgcagatg caacggtttg gcaggtcatc agccagccgg
     3061 cccaatactg gaaagagatc aacatgaaaa agctgactat gaaactgaaa ggcttcatcg
     3121 tagccgaggg catcaacctg agtatcaaca tggactatcc gctgtatgac tgagacctac
     3181 gccataaccg gtaaaagcac caccttgaag caatacgccg atgtgcacga caccattgct
     3241 gccatcaaac gtattgtcaa gaaaaatgcc cccgtattgt acggtctctc tgccgatatc
     3301 gaaggaagaa atgccgtggc cactttccgg aacatctggg attatgtgcg tgaaaacatc
     3361 cggtacaaga atgacgaacc cggcaaagaa cagctccgca ctccccagcg caccctgatg
     3421 gaccgtaccg gcgactgcga cgacatgacg atactcattt ctgcattgct caccaatatg
     3481 ggttacgacc acgagctgtg catagcagcc tataaaaggc ccgaccagtg gcagcacatc
     3541 tatccggcag tgtataacga atacgggaac cgctatgtga tagactgtgt acccgagata
     3601 ccttatttca attacgaagc acaaccgatt aaaaaccaaa taattgtacc catgaaat
//
LOCUS       DAOOQP010000192         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_30587_length_3623_cov_4.501682, whole genome shotgun sequence.
ACCESSION   DAOOQP010000192 DAOOQP010000000
VERSION     DAOOQP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30587_length_3623_cov_4.501682"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1169
                     /gene="mltG"
                     /locus_tag="PLK75_12615"
     CDS             123..1169
                     /gene="mltG"
                     /locus_tag="PLK75_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOY40132.1"
                     /translation="MKKKTRKIGLIILVFFLLILVAGGFTAYHFYKYIYAPNVVEGAA
                     PHFLKIPSGSGFDDVVEILSNEGLIIDTNSFIWVAEKKAYPERVKPGCYQLNPGMNNN
                     ELVNLLRSGKQSPVKLTFNNLRSLEQLAGKAGEVFEEDSAYIAQYLANKLVADSLGFN
                     GSTFIAMFIPNTYEFYWNTPASKFVERMHEEYNKFWTSNRRQKAEKAGLSLVEVSTLA
                     SIVEMETQKNDEKSRIAGVYMNRLQSGMLLQADPTVIYAVGDFSIRRVLLKHTEYDSP
                     YNTYKYKGLPPGPICMPSVSSIDAVLDYEIHDYFYFCAKEDFSGYHNFAKTLAQHNAN
                     ARKFHRPLNERNIY"
     gene            complement(1163..1534)
                     /locus_tag="PLK75_12620"
     CDS             complement(1163..1534)
                     /locus_tag="PLK75_12620"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY40133.1"
                     /translation="MKKILIPAGENSPEVLTDIQEGLFSITGVMCPENPIPFFTRLES
                     CFDEFVYICNREKTVVFQLIYFNSSSARLIYRFLKRISSVGNIRVAWYYEEGDTDLLE
                     SGQDYAELTGLSFDFVAYRPQ"
     gene            complement(1583..1861)
                     /locus_tag="PLK75_12625"
     CDS             complement(1583..1861)
                     /locus_tag="PLK75_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40134.1"
                     /translation="MDKPDIYCPQYSGVNLTKINYNTFKCNYCGCVILNSEMEEFSVK
                     SKNMQFHINAKKSGRKGNYEIAMDDNAKKQFKKIILFVALGMLVVFLF"
     gene            complement(1872..3005)
                     /locus_tag="PLK75_12630"
     CDS             complement(1872..3005)
                     /locus_tag="PLK75_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY40135.1"
                     /translation="MKNPLPEQVYSARVIPREADIRGDYFRDQTAIIHSTAFRRLKSK
                     TQVFYAPDNDHICTRIEHVLHVATISAAICKGLNMHGWELNSEMAYAIGLGHDLGHTP
                     FGHAGEKAINTYLGGGTSFAHEINSYRIVAHTANHGNGLNLTFGVRDGIICHNGERDE
                     QHISPDPSIKDLDLIKDRNVLPSSYEGCIVRLADKIAYLGRDLEDAVLAGIISEKIIP
                     EKIKSQLGTRNGKIINDLVIDLIEASKNSDTIGFSDEKYDLMQSLKEFNYKAIYFHSS
                     LQAYEKFCDKVIRQLYEHFEELFDKLQVNYSDYQNQVCNIDRQFGEYLQGMEVFYNNG
                     ENSGKQRITDFISGMTDTYCIDAARQINIPEPIVFLKKPKCNY"
     gene            complement(3007..>3623)
                     /locus_tag="PLK75_12635"
     CDS             complement(3007..>3623)
                     /locus_tag="PLK75_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOY40136.1"
                     /translation="TRNSSICSNSNSMIKSICVFCASSEDLDELYYEKATEFGRALGR
                     HKLNLIHGGGQIGLMGRLSKAALECSVHVTGIVPEALNKTGIVSEYDSRTIVTRDMMT
                     RKARMRKLADAFVALPGGFGTFEELLEIITLKQLKYHTKPIVILNVNNYFKYLLLQFE
                     TAYNEKFANQQYKQLFLVTDDIEEAIQYIINYRHQNVYDKYLKA"
BASE COUNT         1067 a          725 c          701 g         1130 t
ORIGIN      
        1 taggcttgta ttatcgcagg gctataatga gaagatgata attccactaa gcttatggaa
       61 gccttgtaat caattgaaaa atactatttt tgcagcaata aagccttggt aattcaattg
      121 caatgaaaaa gaaaacacgg aaaatagggt tgataatact tgtatttttt ctcctgatac
      181 tggttgctgg aggatttact gcatatcatt tctataaata tatatatgct ccgaatgttg
      241 tggaaggagc tgccccacat tttctgaaaa tcccctctgg ctcaggtttt gatgatgtcg
      301 ttgaaattct gagcaatgag ggtttaatta tcgatacaaa ctcatttata tgggttgctg
      361 aaaaaaaagc ttaccccgaa agggttaaac cgggttgcta tcaactaaat ccgggaatga
      421 acaataacga actggtgaat ttgcttcgtt caggaaaaca atccccggtg aaacttacct
      481 ttaataactt aagaagtctc gagcaacttg cgggaaaagc gggagaagtt ttcgaagaag
      541 attctgcata catcgcccag tatcttgcaa acaaacttgt tgccgattct ttgggtttta
      601 acggtagcac tttcattgct atgtttatcc cgaatactta cgagttctac tggaatacgc
      661 ctgcatcgaa atttgttgaa agaatgcacg aagaatacaa taaattctgg acttcgaata
      721 gaagacagaa agccgaaaaa gcagggcttt cgctggttga agtgagcacc ctggcatcta
      781 ttgttgaaat ggaaacgcaa aaaaatgatg aaaagtcaag aattgcaggg gtgtatatga
      841 acagattaca aagtggtatg ctgcttcagg ctgaccctac cgtaatatat gcagtgggtg
      901 attttagcat cagaagggta ctgctgaaac acaccgaata cgattcacct tacaatacat
      961 acaaatacaa aggtttacct cccgggccta tttgtatgcc atcggtttcg tcgattgatg
     1021 ctgtgcttga ttatgaaata cacgattact tttatttttg tgcgaaagaa gatttttcgg
     1081 gttaccataa ttttgccaag actctggctc agcataatgc caatgctcgt aaatttcaca
     1141 ggccattgaa tgaacgaaat atctactgag gacgatatgc aacaaaatcg aagcttaatc
     1201 ccgtgagttc ggcatagtcc tgccccgatt cgagtaaatc ggtatcacct tcttcgtaat
     1261 accacgcaac acgaatattt ccaactgagc taatcctttt taaaaacctg taaatcagcc
     1321 tcgccgaact gctgttaaag taaatgagct ggaaaacaac ggttttttcg cggttgcaaa
     1381 tgtaaacaaa ttcatcgaaa catgattcta gccgtgtaaa aaacggaata gggttttcag
     1441 ggcacattac tccggttatc gaaaacaacc cttcctgaat atcggttaac acttcgggcg
     1501 aattttcacc tgcgggtatt aaaatcttct tcatatcaat tagtttagag tattatttga
     1561 taataaatta tggtaaaaag tactaaaaca aaaataccac cagcattccc aaagcaacga
     1621 acagaattat ttttttaaat tgcttctttg cattatcatc cattgcaatt tcgtaattgc
     1681 cttttcgccc tgatttcttt gcattgatat ggaattgcat gtttttcgac ttgaccgaaa
     1741 actcttccat ttcggagttc aatataacgc atccgcagta attacattta aaagtgttgt
     1801 aattgatttt tgtaaggtta actccgctat actgagggca ataaatatca ggtttatcca
     1861 tttattaatt attaataatt acattttggt tttttcaaaa acacgattgg ctcaggtata
     1921 ttaatttgcc ttgcggcatc gatacaatat gtgtcagtca ttcctgaaat aaagtcagtg
     1981 attctttgct ttccgctgtt ttcaccattg ttgtagaaaa cctccattcc ctgtaaatat
     2041 tccccaaatt ggcggtcgat attgcatacc tgattttgat aatcggaata atttacttgt
     2101 agcttatcga ataactcttc gaagtgctca tagagctgcc tgataacctt atcgcagaat
     2161 ttctcgtaag cctgcaaaga gctgtgaaaa tatatggctt tgtagttgaa ttcttttagc
     2221 gattgcatca ggtcatattt ttcatcagaa aaaccgatag tgtcggaatt cttagaagct
     2281 tcaatcaaat cgataaccag gtcgttaatg atttttccgt ttcgggtacc aagctgcgat
     2341 ttaatttttt caggaataat tttctccgaa ataattcctg ccaacactgc gtcctcaagg
     2401 tctcttccca gataagcgat tttatcggca aggcgcacaa tacaaccttc ataggaggag
     2461 ggaagcacat tgcggtcttt tatcaggtca aggtctttga tgcttgggtc gggcgaaata
     2521 tgttgctcat ctctctctcc gttatggcaa ataatcccgt cacgtacacc aaaagtcagg
     2581 tttaatccat tgccatgatt tgccgtatga gctacgatcc tatagctatt aatttcatga
     2641 gcgaaagatg taccaccacc cagataagta ttgatggctt tttctcctgc atgaccgaaa
     2701 ggtgtatgcc ccagatcgtg tccgagccca atggcataag ccatttcgga gttcagttcc
     2761 cacccatgca tattcaagcc cttgcaaatt gccgctgaaa tagttgctac atgaagcacg
     2821 tgttcaatgc gggtgcatat atgatcgttg tcgggagcat aaaatacctg tgttttactt
     2881 tttagcctgc gaaaggcagt actgtgtatt attgcggtct gatctctgaa gtagtcgcca
     2941 cgaatatcgg cttctctagg tataacacgt gctgaataca cctgctcagg taatgggttt
     3001 ttcatatcat gcctttaaat atttatcgta cacattctga tgcctgtaat taataatgta
     3061 ttgaatggct tcctcaatgt cgtctgtaac taagaaaagc tgcttatact gctggttagc
     3121 aaacttttcg ttataggctg tttcgaattg caacagcaaa tatttgaaat agttgtttac
     3181 attcagaatt acaatcggct ttgtgtgata ttttaattgc ttcaaggtaa ttatttcaag
     3241 gagttcttca aaagtgccga atcctcccgg taaagcaaca aatgcatccg cgagttttcg
     3301 catacgggct ttgcgtgtca tcatgtcgcg tgtaacgatg gttctgctgt cgtattccga
     3361 aacaataccg gtcttattta aggcttcagg tacaattcct gtaacatgca cactacattc
     3421 aagagctgct ttcgacagcc gacccatcag gcctatctga cccccgccat gaattagatt
     3481 gagtttatgc ctgcccagag ctctgccaaa ttcagttgct ttttcgtaat acaattcatc
     3541 aagatcttcg ctcgaagcac aaaacacgca aatactttta atcatactgt tactgttact
     3601 acaaatggaa ctgtttcggg tag
//
LOCUS       DAOOQP010000193         3574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_31167_length_3574_cov_4.580278, whole genome shotgun sequence.
ACCESSION   DAOOQP010000193 DAOOQP010000000
VERSION     DAOOQP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31167_length_3574_cov_4.580278"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1559)
                     /locus_tag="PLK75_12640"
     CDS             complement(153..1559)
                     /locus_tag="PLK75_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOY40137.1"
                     /translation="MITTSKIHHRGEDRLKLDFAYDTSISDKIRQIPGAAWSRTHKAW
                     LIPDDAESRNQFGSLFPEITLPLVKNQPEIEDGSKTNEKDLAFYSNDLSMRTQVRILI
                     NYKYIRVKCPKNENDIQFFRSIKYCYWKPDGFYWEMPNSEDNYEMVLTHFNNRTYSFE
                     EENYVPANTEKKKSVSFELKVREISVDGLEKIEEQIRILRNWMNHKRYSDSTIDTYCE
                     SVRIFLKYVYPKTATEVEVDDMVLFVNDFIIGNRYSYSYQNQVVNGCKLFFREVIKSR
                     MDVEKFERPRREHKLPNVLSKGEIKAILTSSTNVKHRTMLSLIYACGLRRSELLNLKV
                     AAIDSNREVLYILNSKGKKDRVVPLSKSLMTMLETYIEMYKPKDWLFEGQIKGEPYSA
                     TSLSEVLGAAVLKANIKKPVTLHWLRHSYATHLLESGTDLRYIQELLGHKSSKTTEIY
                     THVSTKSLKLIRSPFDDL"
     gene            complement(1660..2832)
                     /locus_tag="PLK75_12645"
     CDS             complement(1660..2832)
                     /locus_tag="PLK75_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40138.1"
                     /translation="MKKLTLWACILLPAISIHAQIIPVYTEHFTSYDNVWKQCNNESG
                     TLSVTDSKYKIGTRLENGQWLHTIENKKIDAEHDFTIEAAMMHTGGAGNQFYGITYGH
                     SDDGNAYNFCISEEGKYAIIKNEQGTTEELASGTALLYINKADSAVNVLKITKTNGNC
                     MYYVNHSLIATLPFTVFFGPNLGFAVAGTQSVEVDYLSIAVELPNTVTGIAIPDGMVN
                     ILNEEFDATPNGWSKKCSSGSFNTGQGRAIVKCSGIGRCCSVYTETSIHQQGDFIVEA
                     LVSKTDGASYTSFGLMSGSENMEISYEWFLQSTGAFGFYVDYSGDTYDTPEVNTYNAP
                     NMLSIYKTGNLVKFYINGKEVFTTDYIASMFDEIGFVVCDPGTIAVENIQIWETKP"
     gene            complement(3076..3366)
                     /locus_tag="PLK75_12650"
     CDS             complement(3076..3366)
                     /locus_tag="PLK75_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOY40139.1"
                     /translation="MEKGGFVYIITNSNNTVLYTGVTNNLNRRVIEHKEKLSPKSFAS
                     KYNCNKLVYFEYFQSIEEAIFREKQLKAGSRKKKIDLINGINPTWDDLSIEN"
BASE COUNT          938 a          755 c          669 g         1212 t
ORIGIN      
        1 tattaaggtg gtatttccat tatcggggtc ttgtctttta agctgacgca caactagttt
       61 ataaaaacgg aaaaaccatt tttatacagc taaattctat tgtctttgta cggttcattc
      121 ttgtttttat ttcttccaaa tttagtcgtt ttttacaaat catcaaatgg agatctgata
      181 agttttaaac ttttggttga tacatgtgta tatatttcgg tagtcttgct gctcttatga
      241 cctaaaagct cctgtatata cctcaaatca gtgcccgatt ccaataaatg tgtcgcataa
      301 ctatggcgca accaatgtaa tgtaaccggt tttttaatat ttgcttttag caccgcagcc
      361 cctaaaactt ctgacaaact tgtcgccgaa tatggctctc ctttaatctg cccctcaaat
      421 agccagtctt tgggtttgta catttcaatg tatgtttcca gcatggtcat aaggcttttc
      481 gataatggaa ctacccgatc tttctttccc tttgaattca aaatgtatag gacctcccga
      541 tttgaatcaa ttgcagccac ttttaaatta agtagttcgc tcctgcgcaa accacaggca
      601 tatatcaaac ttagcatggt tcggtgcttt acgtttgttg aactcgttaa tattgccttt
      661 atttctcctt tactcaatac attcggaagc ttgtgttctc tccgtggccg ttcaaatttt
      721 tccacatcca ttcggctttt tatcacttcc ctaaaaaata atttacaccc gttaacaacc
      781 tgattttgat atgaataact gtatctgtta ccaatgataa aatcattgac aaacaaaacc
      841 atatcatcaa cttctacttc ggtcgcagtt ttgggatata catattttaa aaaaattcta
      901 accgactcgc aataggtatc gatggttgag tcactatacc gcttatggtt catccaattt
      961 cgaagtatcc tgatttgctc ttcgattttt tctaatccat cgacgctaat ttcccgtact
     1021 tttagttcaa aagaaacaga ttttttcttc tctgtatttg caggcacata attctcttct
     1081 tcaaaactat atgttcggtt gttaaaatgt gtcaaaacca tttcataatt gtcttcactg
     1141 tttggcattt cccaataaaa accatctggc ttccagtagc aatactttat actcctgaaa
     1201 aactgtatgt cgttttcatt tttgggacat tttacccgta tatacttata attgattaaa
     1261 atacgaactt gggttcgcat cgaaagatca tttgaataaa aagccaaatc tttttcattc
     1321 gttttgcttc cgtcttcaat ttcaggctgg ttttttacaa ggggcaaggt gatttcggga
     1381 aagagtgacc caaattggtt gcgtgactct gcatcatcgg gtatcagcca cgctttgtgt
     1441 gtccggctcc atgctgcccc cggaatttgc ctgattttat cggaaatgct tgtgtcgtaa
     1501 gcaaaatcaa gctttaaccg gtcttctccg cgatggtgta ttttactggt tgtaatcatt
     1561 gtttgtggcg gttatatttc gtattggtgg ataggtggaa taaatggtga acatataaac
     1621 ataatccatt ctttggcatt ttttttctgt gcctgagaat catggtttgg tttcccatat
     1681 ctgtatattt tcaaccgcaa tggttccggg gtcgcacacc acaaaaccta tctcatcgaa
     1741 catcgatgca atgtagtcgg tggtaaaaac ttccttaccg ttaatgtaga attttaccag
     1801 attgccggtt ttatatattg acagcatatt gggtgcatta tacgtattta cctcaggagt
     1861 atcgtatgtg tcacctgaat aatccacata aaaaccgaag gctccggtcg attgcaggaa
     1921 ccactcataa ctaatttcca tgttttcgga acccgacatt agtccgaagg aagtatagct
     1981 tgccccatcg gttttagaca ctaaggcttc cacgatgaaa tcaccctgct ggtgtattga
     2041 tgtttcggtg taaacactgc agcaacgtcc gataccgctg cattttacta tcgctctgcc
     2101 ctgaccggtg ttgaacgacc ctgaactgca ttttttactc cagccattcg gggtggcatc
     2161 gaattcttcg ttgagtatgt ttaccatacc gtcgggtatg gcgattcctg taaccgtgtt
     2221 cggtaattct acggcaatgc ttaggtaatc aacctctacc gactgagttc cggcaacagc
     2281 aaatccaagg tttggtccga aaaacacggt aaatgggagg gtggcaatca ggctgtggtt
     2341 cacataatac atgcagttgc cattggtttt ggtaatcttc agtacattaa cagcgctgtc
     2401 tgctttgttg atatacaaca acgctgtgcc tgaagcgagt tcttctgttg taccttgttc
     2461 gtttttaatt atggcgtatt tgccttcttc cgaaatgcag aaattatatg cattgccatc
     2521 gtcgctgtgc ccataggtga ttccatagaa ctggttgccg gctccccctg tgtgcatcat
     2581 ggctgcttca atcgtaaaat catgttccgc atcaattttt ttattttcta tggtatgcaa
     2641 ccactgcccg ttttccagcc gggtgccgat tttatatttg ctatcggtta cactcagtgt
     2701 accagattcg ttgttgcatt gtttccacac gttgtcgtac gatgtgaaat gctctgtgta
     2761 cacggggatg atctgtgcgt gaatgctgat tgcaggcaaa agaatgcatg cccaaagggt
     2821 gagttttttc atatgctgat agtttaccat ggttacggtg ttgaaccgtt gggttgattg
     2881 tttagcttca gccactaaat tatgtaaaaa atcgacacat tattcaacgc atacgatgaa
     2941 tagtaatttt attttcaatg acgctgggga gcccgtcaaa tacagattgc ttcatgttcg
     3001 tacctcacat tcgcaatgac gggcgcaccc aacgtcattg cgaacgcagt gaagcaatcc
     3061 acccatacat agacgttaat tctcaatact taaatcatcc caagtgggat taatgccatt
     3121 aatcaaatcg atttttttct tgcgagaacc tgcttttagt tgcttttctc gaaaaatagc
     3181 ttcttctata ctttgaaaat attcaaaata aacaagctta ttgcaattat actttgatgc
     3241 gaacgattta ggagataact tttctttatg ttctattacc ctcctgttaa gattattagt
     3301 aacaccagta tataaaacgg tattattgct gttggtgata atataaacaa aacctccttt
     3361 ttccatggaa taaaaatagt aattatttta taatacagtt tttaaactcg cccgtcaaat
     3421 gcagattgct tcatgttcgt acctcacatt cgcaatgacg ggcgcaatga gcctgtcatt
     3481 gtacagcctg cttcatcgca ctgcggacac agaggctact cgaagtgacc gcagtgacgg
     3541 gcggggattg taattacttc gtgctgttca gctc
//
LOCUS       DAOOQP010000194         3559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_31327_length_3559_cov_3.905822, whole genome shotgun sequence.
ACCESSION   DAOOQP010000194 DAOOQP010000000
VERSION     DAOOQP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31327_length_3559_cov_3.905822"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLK75_12655"
     CDS             complement(<1..378)
                     /locus_tag="PLK75_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40140.1"
                     /translation="MRLVPNTILHEKRCSGLKQLRYRIQVFAFILISGISYGQQSFFS
                     DMNYGLEYDLMAPRIGKWYSVNLSAWAGKQHFKHSLVFVNININSRHLTEESFSYDKL
                     TAGGYRLEYYFNPELKYFSLGLLL"
     gene            complement(535..>3559)
                     /locus_tag="PLK75_12660"
     CDS             complement(535..>3559)
                     /locus_tag="PLK75_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY40141.1"
                     /translation="TATQTYTATIIYPCDTMYASTTVQVINPPIVDLGPDFEITGFTA
                     PLDADMPSGNTGIWSYVTGSGPGTVTIGSPTNPHTIGTVSEFGTYTYTWTETSLAPNC
                     VSSDDIAITYFHIPTTDFYITPVLCFGDTAVITYTGNGFDWATYNWDFDGATVLSGTG
                     WGPYQVVWDYHGLHNVSLAITELNVTVDTVISVYVPPILDYEFTYSDDPCYHSCNGYA
                     QVDVTGGTPPYEYHWGSSTNIMNHLCAGQYIITVDDVNGCSFTQQFTINEPTQMTFDT
                     AYQNVDCFGNHTGWATVTANGGTPPYTYIWSDMFNGATHNDLAAGIYTVTIYDSNNCS
                     LFEQFHITQPGLLQIATSPSLAICENTSIQISAQQIGGVQPYTYHWNTGSGFSNGPPS
                     FEITPHQDVTYQVYITDGHNCQTPTNTIAITVSPTMSYELAFQNVSCNGVCDGHAEIT
                     VLGGIPPFSYSWASANHIYNNLCAGLYVLTVTDEIGCNVHTHFVITEPEQLEAYTTSS
                     PANCFNSNDGQAVVHVSGGTLPYTYLWPDFSNTNVMVNGAGSYVVTVIDAHNCRVEAT
                     TTITSPPLLVVQAIPDQVICHGGEATVSAQSAGGNGYTQFLWQGSDGSEYHTHLFHAT
                     PEATTTYALTVTDENGCTAAESVMVTVLPDLSIESIIANTDSICAGDSVIITLHATGG
                     NGGPYTLTLQNGITVPSPYKVFPEETTTYYFTLSDDCESPTVTDSVTIYVMPAPENEF
                     IVDKISGCEGDMFIFTETHENDGNTYSWNFGDGNFADIQNPYHIYQDPGIYSVSLTIT
                     NEFNCKTSVVISDAVEIFNKPEANFIATPEETTILNPLISFTNLSEDATYLFWFYGDG
                     DSTVYIHNPQHFYSETGEFQVQLVVENIHGCADTVYRTVNIIGEVTIYAPEAFTPNGD
                     GINDCFRLCGTMIDPYSFNMTVYDRWGERVFETDEYRTDVSCNACTSDSWDGTRGSRV
                     AGDKYLPMGLYYWYAEFRDIIGIKHEVSGMVRLIR"
BASE COUNT         1048 a          742 c          748 g         1021 t
ORIGIN      
        1 taaaagtaaa cccaaactaa aatactttag ttccggatta aaatagtatt ctaaccggta
       61 acctccggca gtaagcttat cgtaagaaaa agattcttcg gttaagtgac ggctatttat
      121 attgatattt acgaatacca gagaatgttt aaaatgctgc tttcctgccc aggcacttaa
      181 gttcacacta taccactttc cgatacgtgg tgccattaaa tcatactcca acccataatt
      241 catatcactg aaaaacgatt gttgtccgta tgaaattcct gaaatcagaa taaaagcaaa
      301 tacttgaatt ctgtagcgaa gctgttttaa accagagcat ctcttttcat gtaaaattgt
      361 attcggtact aaacgcatag agtaaaatca actattcgct aaaatcgaaa ctactactta
      421 ttaatttaat agattagtca aacaaaagta atgattatta cttaatctta ttgtaaatta
      481 catgaaatct atcaaccttc tgctgcaatg ataatatttt catcaaatag agtactatct
      541 aatcaggcgt accatacctg acacttcatg ttttattcca ataatatctc tgaattcggc
      601 ataccaatag tacaagccca taggcagata tttatcacca gcaacccttg atcctctggt
      661 accgtcccaa ctatcagaag tgcaggcatt acacgaaaca tcggttctgt actcatcggt
      721 ttcgaaaact ctttcacccc agcgatcata aactgtcatg ttaaaagagt aagggtcaat
      781 catagttccg cataacctga aacaatcgtt aataccatca ccattgggtg tgaaggcttc
      841 gggagcataa atggtaactt ctccaataat atttacggtg cggtacactg tatcggcaca
      901 tccatgtata ttttcaacaa cgagttgcac ctgaaattcg cctgtttcag aataaaaatg
      961 ttgtgggtta tggatatata ctgttgagtc accatcgcca tagaaccaga ataggtaggt
     1021 ggcgtcctca ctcagatttg taaatgaaat aagaggattt agtatcgttg tttcttcggg
     1081 cgtagcaata aagtttgctt cgggtttatt aaagatttca acagcatcgc taattacaac
     1141 actggttttg caattgaatt catttgtaat agttaagctt actgaataaa ttcctgggtc
     1201 ctgataaata tgatatgggt tttgaatatc agcaaaattc ccatcgccaa aattccagga
     1261 atatgtattt ccgtcattct catgggtctc ggtaaatata aacatatctc cctcgcatcc
     1321 tgaaatttta tcaacgataa attcattctc aggagccggc atcacatata tggtaactga
     1381 atcggtaact gtgggagatt cgcaatcatc ggacaatgta aaataatagg tcgttgtttc
     1441 ctcaggaaaa actttgtaag gagaaggaac tgtaataccg ttctgaagcg ttaaggtgta
     1501 aggacctccg ttacctccgg ttgcatgaag cgtaataatt actgagtctc ctgcacaaat
     1561 agagtcggtg ttagcaatga tgctttcgat tgaaagatcg ggtaacacag tgaccataac
     1621 actttcagct gccgtacatc cattttcatc agtcactgtg agtgcatatg tcgttgttgc
     1681 ttccggtgta gcatgaaaca aatgtgtatg gtattcggaa ccgtcggagc cttgccagag
     1741 gaattgcgta tatccgttac ctcctgccga ttgtgcactc acagttgctt cacctccgtg
     1801 gcatataacc tgatcaggta ttgcctgaac aacaagtaac ggcggagagg taatcgtagt
     1861 ggttgcttcc accctgcagt tgtgtgcatc tataaccgta acgacatatg agccagcacc
     1921 atttaccata acattggtat tggagaaatc aggccataag taagtatatg gtaaggttcc
     1981 accagaaaca tgcactactg cctgcccgtc gttgctgtta aagcagtttg cagggctgct
     2041 ggtcgtgtag gcttccaact gctcgggttc ggttattacg aaatgagtat gcacattgca
     2101 gcctatttcg tcagtaacag taagcacata caaaccagcg cacaaattgt tgtagatgtg
     2161 gtttgctgaa gcccacgaat atgaaaatgg tggtatccct cccaaaacag taatttcagc
     2221 atggccatcg caaactccgt tacagcttac attttggaat gccagttcat agctcattgt
     2281 tgggctaact gtaatggcta ttgtattggt tggagtctgg caattatgtc catcggtgat
     2341 atacacctga tacgttacat cctgatgtgg tgttatttca aaggacggag ggccattaga
     2401 aaacccgctt cccgtattcc agtgatacgt atagggttgc acccccccaa tttgctgggc
     2461 agatatttga atactggtat tttcgcagat ggctaggctt ggagaagtcg caatttgcag
     2521 caatccgggc tgcgtaatat ggaattgttc aaacaaagag cagttgttac tatcgtaaat
     2581 ggtaacggta tatattccgg cagccaaatc gttgtgcgtt gcaccattga acatatcgct
     2641 ccaaatatat gtgtaaggag gcgtaccccc gttggcagta acagttgccc atcctgtatg
     2701 atttccaaag cagtcaacat tctgatatgc ggtatcaaaa gtcatttggg tgggttcatt
     2761 tattgtaaat tgctgagtga atgaacagcc gtttacatca tccactgtaa taatatactg
     2821 tccggcacaa aggtggttca ttatgtttgt tgaactcccc cagtgatatt cataaggtgg
     2881 tgtgccaccg gttacatcaa cctgagcata tccgttacat gaatgatagc aagggtcgtc
     2941 gctgtaagtg aattcataat ctaatatcgg gggcacataa accgaaatca ccgtatcgac
     3001 agttacatta agttcggtta tggcaagact gacattgtgc aaaccatgat aatcccatac
     3061 cacctgatat ggtccccagc cggtaccact aagaactgta gctccatcga aatcccagtt
     3121 ataggtagcc cagtcgaagc cattgcctgt atatgttata acggctgtgt cgccaaaaca
     3181 caaaaccggt gtaatataga agtcggttgt cggaatatga aaatatgtaa tggcaatatc
     3241 gtcggagcta acacaattgg gggcaaggct tgtttccgtc caggtgtagg tgtaggtgcc
     3301 aaattccgaa acggtaccga tagtgtgggg gttcgttgga gagccaatcg taacagtacc
     3361 gggcccactt cccgtaacgt aactccaaat acccgtattc cctgaaggca tatcagcatc
     3421 gagaggtgcc gtgaatccgg ttatttcaaa atcgggaccc aggtcaacaa tcggagggtt
     3481 aatcacctgc acggtagtgc tggcatacat ggtatcgcag gggtaaataa tagtggctgt
     3541 gtaagtctgc gttgcagtt
//
LOCUS       DAOOQP010000195         3509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_31931_length_3509_cov_5.190504, whole genome shotgun sequence.
ACCESSION   DAOOQP010000195 DAOOQP010000000
VERSION     DAOOQP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31931_length_3509_cov_5.190504"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1365
                     /locus_tag="PLK75_12665"
     CDS             313..1365
                     /locus_tag="PLK75_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40142.1"
                     /translation="MRYLSNLLLLIATICSGNAGFSQHGTKDWEWEWAPVGAVWLYET
                     ANMGTNNVYAQYWYVRSAKDTLFHGHNCRKLEVERHYWPGFEPVKMPDRFTYQDGGNI
                     YFYNADIGEFVLSFSYDIQHGDTVTWQMPVFEDCINSFSLDSVFIWDAGFLSGEGMTY
                     TPYSVFMFSNIPIYSYKVGYSHNNCYAGEIGYYSTFLQHNGYGRFFDYYGIMSDIFEL
                     SILNEMQNSCMCYYDGNITINRASVSIYNDTIIYYQDSCLNYYNKFNSISSHTGEKLL
                     QVYPNPFTDLICVDLPASADGYGIRVYDLTGQLLFSAKIPGNSAPVNLSRLAPGTYLL
                     QIQTNHNVFTQRIIKN"
     gene            1372..2373
                     /locus_tag="PLK75_12670"
     CDS             1372..2373
                     /locus_tag="PLK75_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY40143.1"
                     /translation="MKRLFILSAFFLYAATSMAQTVSDTVIIAWKKKNLTTQQETLFD
                     TLQLQIYNLNDVRYFKKVTPGYTAWNNYYHIRCSASADTVFGILGKSGLFDTIYYDKK
                     VGVECSNPVATNDPLNNTYVNDMLGLPCAWSLTTGNPNHTVGLIDSDFQQNLHSDLWG
                     KVNDSYETGDFTSGGYYKGSVPPAPGTDSYHGLVMSGIIAAIAGNSLGLCGAAYNTKL
                     NLYDYSTAGSGNYHSEVTSALNFAAQNGEKVVNISLRYCAGENEDFVSFHNWVVNQGT
                     TVVYSAGNEREDYLGETSLMYEGVYEIPGIIIVSSVNEDNKTYIGANNSNYRCNRHF"
     gene            complement(2555..3181)
                     /locus_tag="PLK75_12675"
     CDS             complement(2555..3181)
                     /locus_tag="PLK75_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40144.1"
                     /translation="MVFKSTEGQHLYWKFVKYETIKEYVSGINYLSSQGINIKGIVCD
                     GRKGLFNALQCYPIQMCQFHQVAIVTRYITRNPKVQASIELKKLMHLLPQTDKESFWG
                     ALEQWHTKWEDYLNERTVNPDTGKSRYVHKRLRSAYRSLESNKRHLFTWYDNMELGLP
                     NTNNQLEGTFTDLKNKLRNHNGLSQKRKEKFINGFFKASVIKVSKQKS"
BASE COUNT         1031 a          693 c          676 g         1109 t
ORIGIN      
        1 tgtcaaagaa caaagaaatt tatttagtga ctaaatttta acgcatcagt agtaagtcgc
       61 aataaatagt tgccaactat ttacaaaaga gttgggttgg taaagaaata ctcttgttaa
      121 tacataccat ctttccttta ataaaaatgt ttatttttat aataaataga aaagtataat
      181 gaaggtaacc cgttgtgctt ttagtccaaa tttagtttaa ctttgtataa ctgtattaat
      241 tgaacgaaaa tgatggttct tgaaaaacaa acaggcgtgg ttgatcaaag ttaaatacaa
      301 agctaaggaa gcatgagata cttaagtaac ttactattat tgattgccac aatttgttcc
      361 ggcaatgccg gtttttctca gcatggcacc aaagactggg aatgggagtg ggcgccagtt
      421 ggggctgtat ggttgtacga aacagccaac atgggcacga acaacgttta tgcacaatac
      481 tggtatgtac ggtcggcaaa agacaccctt tttcacgggc ataactgccg taaactggaa
      541 gtcgaacgcc actactggcc cgggttcgag cccgtaaaaa tgcccgacag gtttacttac
      601 caggacggtg gcaacattta tttttacaat gccgacatcg gtgaatttgt actctcgttc
      661 agctacgata ttcagcatgg cgacacggta acatggcaaa tgcccgtgtt cgaagattgt
      721 ataaactcat tttctcttga ctctgtattt atttgggatg caggtttctt atctggagaa
      781 ggaatgacat atactcctta ttctgtgttt atgtttagta atattcctat ttattcgtat
      841 aaagtaggtt actcacataa caattgttat gcaggagaaa taggatatta ttctaccttt
      901 cttcagcata acggttatgg tagatttttt gattattatg gaataatgtc tgatattttt
      961 gaattatcaa ttttaaatga gatgcaaaat agctgcatgt gttattatga tggaaacatt
     1021 acaataaatc gtgcatcggt aagtatttac aatgatacca taatctatta ccaggactcc
     1081 tgtctgaact attacaacaa gtttaatagc atctcatcgc ataccgggga aaagttgctt
     1141 caagtttacc ccaacccctt tacagacctt atctgtgtcg accttcccgc ttcagccgac
     1201 ggctacggca tccgtgttta cgatcttacc gggcaactcc tcttttcagc taaaataccg
     1261 ggaaacagcg ctcccgtaaa cttaagccgg cttgctcccg gaacctacct cctgcaaata
     1321 caaacaaacc ataatgtctt tacccaacgt ataatcaaaa actaaagaat catgaaacga
     1381 ctatttattt tgtcggcatt ttttctttat gccgcaacgt caatggcgca aaccgtaagc
     1441 gatacggtta tcatcgcatg gaagaaaaaa aatctgacca cgcagcagga aacgctattc
     1501 gacaccttgc aattgcaaat atacaatctg aacgatgtac gatatttcaa aaaagttaca
     1561 ccgggctata ctgcatggaa taattactac catatcaggt gctcagcgag cgctgacact
     1621 gttttcggca tcctcgggaa atcaggtttg ttcgacacaa tttattatga taaaaaagta
     1681 ggggttgagt gtagcaatcc tgttgcaaca aacgatccgt taaacaacac ttatgtaaac
     1741 gacatgctgg gattgccatg cgcatggagc ctgactaccg gaaatccgaa ccataccgtt
     1801 ggtcttattg acagcgattt tcaacaaaac cttcactccg acttatgggg taaagtaaac
     1861 gattcctacg aaaccggcga tttcacatcc ggtggctatt acaaaggttc cgtaccgcca
     1921 gcacctggca ctgattctta ccatgggttg gtaatgtcgg gtattattgc ggctatagcc
     1981 ggtaatagcc tgggtctatg cggcgcagca tacaacacaa aacttaacct gtacgattat
     2041 tccacggcag ggtcaggcaa ttaccattca gaagtaacca gcgccctgaa ttttgccgct
     2101 caaaatggtg aaaaagttgt caatattagt ttgcggtatt gtgccggaga aaatgaagat
     2161 tttgtaagct ttcacaattg ggttgttaat caggggacaa ctgtggtata tagtgctgga
     2221 aatgagagag aagattatct aggagaaact tcacttatgt acgaaggggt ttatgaaatt
     2281 cctggaatta taatagtaag ctctgtaaat gaagataaca aaacatatat tggagcaaat
     2341 aatagtaatt atcggtgtaa tagacatttc taatgctttt ttttagtagt tagtctctaa
     2401 tagacatttc taatgctttt tattttcagc aatttctgtt tttaggttta tttggagtta
     2461 tatttgtcaa tcaggtaagc ggaaacaggc tctgctgggg agcaacctgt caggaatacc
     2521 ctgatttgtc gaagtatcag aaagctggca taggtcagct tttttgtttt gatactttga
     2581 taacagatgc cttgaaaaac ccatttataa acttttcttt tcgtttctgg cttagcccat
     2641 tatgattacg tagcttgttt ttcaaatctg taaatgttcc ctcaagctga ttgtttgtat
     2701 tgggcaaccc cagttccata ttgtcatacc aggtaaataa gtgccttttg ttgctttcca
     2761 aactccgata agcgcttcgt agtcttttat gaacatatct gcttttaccg gtatcaggat
     2821 taaccgtacg ctcgtttaaa taatcttccc attttgtatg ccattgttca agagcccccc
     2881 aaaagctttc tttatcggtt tgtggcaaca agtgcattaa tttttttaac tcaattgaag
     2941 cctgaacttt tgggttccga gttatatatc tggtaacaat tgccacttga tgaaactgac
     3001 acatttggat tgggtagcat tgcaaggcat tgaatagacc tttccttcca tcgcatacaa
     3061 ttcctttgat gtttatgccc tgagagctaa gataattgat ccccgaaacg tactccttaa
     3121 tggtctcata cttaacaaac ttccaataca agtgttggcc ttcggtgctt ttaaacacca
     3181 tgacaccaaa gtttctccca aaatatgtcg tatccatcag aataatactc tctttgtgtt
     3241 tatgaaagac ttgggtcaca ttaactttgt ctagtctgcg ttgaattgtt cgtacagaaa
     3301 ccccatagcg gtcagagagc tgtctatatg tctgtttccc ttctgtgtat tcttgccaaa
     3361 gtaattggtt gtttatacgc ttccctgcta aaaactgccg tttgcaatcg aaacatcgat
     3421 agttctgaac tccgttattt gtcccatttt tcttggtcct ttttgacccg caataaatgc
     3481 aaatttttta ttcatcgttt tcaaatgca
//
LOCUS       DAOOQP010000196         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_32350_length_3474_cov_4.028956, whole genome shotgun sequence.
ACCESSION   DAOOQP010000196 DAOOQP010000000
VERSION     DAOOQP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32350_length_3474_cov_4.028956"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLK75_12680"
     CDS             <1..847
                     /locus_tag="PLK75_12680"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOY40145.1"
                     /translation="VIITVTCNSSGGQPVSIENIKAVSEMSHAYGIPVLFDSARFAEN
                     AYFIKMREKAYSHLSIKRIVREMYKYGDMTTMSAKKDGIVNIGGFIACNDLELYRRAS
                     TFNIMFEGYVTYGGMSGRDMAALAVGLNESTEFDYLETRIKQVEYLGDKLISYGIPIQ
                     RPIGGHAIFVDASRILSKLPKEQLRAQTLGIELYLEAGIRGVEIGTLLADRDPETREN
                     RYPDLELLRLAIPRRVYTNNHMDVIAAALKNVFDRRDSITKGYNITWEAPIMRHFTVE
                     LEKVK"
     assembly_gap    1013..1112
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1537..>3474
                     /locus_tag="PLK75_12685"
     CDS             1537..>3474
                     /locus_tag="PLK75_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40146.1"
                     /translation="MKNLSLLLLAASILVCTSRVHAQEAVIQWQKCYGGSNIDNARCI
                     QQTIDGGYIVAGYTYSNDGDISGFHGASDIWVIKIDNVGNIIWQKCLGGSSSDVASTI
                     TQTLDDGYIVAGITFSNDGDVTGCSEDCNSWIIKLDNSGNIQWQNSILALYWAEIGSI
                     QQTIDEDYILCGTAMTDLENGYYDACIVKLDNTGNIQWYKFIGGFDDDYSNTIVLTTD
                     GGYIFSGYTESNNGDVSGNHGGQDAWVVKLDDTGNIEWQKCLGGSSNDKARSIQQTSD
                     GGYIVAGNTFSNDGDVSGNHSGNSDAWVIKLEDTGNIQWQKCLGSNGDEYAFSVQKTT
                     DGGFIVESNIYSNNADIWDYHGGGDAWVVKLENTGNIEWQKCLGGSDDDYAYSIQQTT
                     DGGYILAGYTDSNDEDVSGNHGGPNDAWVVKIIESNVSGLVFHDTNLNGIPDEGEQGI
                     AGHLVKLEPGPFYTFTNNEGKYFFRAEAGDNIVSYVPVNYWYATAEEDYQFSVISNTQ
                     IIDTLDFGIATRENSPDVSVYLTGSPTRAGFDTHYWISYKNQGTLTTSGTVNVAFDPL
                     LTFVSSTEAPTSQLGNVFTWNYGPMGPGVEHQFRVDFEVPGIDYLGDTLFSHVWITPL
                     DPDTCLVNNYDTIYQEITGSYD"
BASE COUNT          976 a          656 c          774 g          968 t
ORIGIN      
        1 tgttatcatt acagttacct gtaattcatc gggtgggcaa cctgtaagca tcgaaaacat
       61 taaagctgtt tcagaaatgt cacatgctta tggaattccg gttcttttcg actcggcacg
      121 ctttgccgaa aacgcttatt tcatcaaaat gagggaaaaa gcttactctc atctgagcat
      181 taagcgaatt gtccgtgaga tgtataagta tggcgatatg actaccatga gcgcaaagaa
      241 ggatggtatc gtaaatattg gagggtttat tgcctgtaac gatttggaac tttatcgtag
      301 ggcatctaca ttcaatataa tgttcgaagg ctatgttacc tacggtggta tgtcgggccg
      361 cgatatggct gccttagctg tcggtttgaa tgagagcacc gaattcgatt acctcgaaac
      421 gcgaatcaag caggtggaat atttgggtga taaactgata tcatacggca ttcccattca
      481 gcgcccgata ggcggacatg caatttttgt ggatgcatcg cgtattctgt cgaaactgcc
      541 caaggaacag ctcagagccc agaccttggg tattgaactc taccttgaag ccggaataag
      601 aggagtggag ataggcacgt tgcttgccga ccgagatccg gaaacccgcg agaatcgtta
      661 tcccgatttg gaactgctgc gtctggctat tccccgcagg gtgtacacca acaatcacat
      721 ggacgtgatt gctgctgccc tgaagaatgt gttcgaccgt cgcgacagta ttaccaaagg
      781 ctataatatc acatgggaag cgccaattat gcggcatttt accgtggaac tggaaaaagt
      841 taagtaattg gttctaacct gattcatttt aataaaagag gctgtcccgg aagggcagcc
      901 taacaacgtc attgcgagcg cagtgaagca cttgtgctga gcgaagttta agcaatctcc
      961 atacccgtag tcgctactat cattgcccgt caaatgcctg tgttggcctg tcnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaataca gattgcttca tgttcgtacc
     1141 tcacattcgc aatgacgggt tctcccaaaa cgtcattgcg agcgcagcga agcacttgtg
     1201 ctgagcgaag tcgaagcaat ctccataccc gtagtcgcta ctatcattgc ccgccaaatg
     1261 cctgtgttga gcctgtcgaa atacagattg cttcatgttc gtacctcaca ttcgcaatga
     1321 cgggttctcc cacaacgtca ttgcgagcgc aaagaagcac ttgtgctgag cgaagtcgaa
     1381 gcaatccacc ataacagagg catttcgcct attttaagaa ccccagttca ccttcttccc
     1441 agcaaattaa aatataaata actttaaaat gctaaggaaa atagatattt tatgtacatt
     1501 aattcgtata tttgccaata gctaacatgc caaagtatga aaaacctttc cctccttctc
     1561 cttgccgcaa gcatactcgt ttgtacaagc cgtgttcatg cacaggaagc cgtaattcag
     1621 tggcaaaaat gttatggggg tagtaacatt gataatgccc gatgcattca acagactata
     1681 gatggtggat atattgttgc aggatacacc tattccaatg atggtgacat ttctggattt
     1741 catggtgctt ctgatatatg ggttataaaa attgataatg tcggaaatat tatatggcaa
     1801 aagtgtttgg gtggtagtag ctctgatgtt gcttcaacca ttacacaaac tttagatgat
     1861 ggatatattg tggcaggtat aaccttttct aatgatggtg atgttacagg atgctctgag
     1921 gattgtaatt cttggattat aaaactggat aattctggca atattcaatg gcaaaatagt
     1981 atattggctt tatactgggc ggaaattgga tctattcagc aaactataga cgaagactat
     2041 atcctttgtg ggactgctat gactgatctc gagaatggct attatgatgc atgcattgta
     2101 aaacttgata atactggtaa tattcaatgg tataagttca taggtggatt tgatgatgat
     2161 tattcaaata ccattgtctt aacgacagat ggaggttata ttttttctgg ttatacagaa
     2221 tctaataatg gtgatgtttc gggaaatcat ggtggacaag atgcatgggt tgtaaaatta
     2281 gatgataccg gcaatataga atggcaaaaa tgtttgggtg gtagttcaaa tgataaagct
     2341 cgttccattc agcaaacatc cgatggcggc tatattgtag caggtaatac attctctaat
     2401 gacggagatg tttcaggaaa tcatagtgga aatagtgatg catgggttat aaaattagag
     2461 gataccggca atatccaatg gcaaaaatgc ttgggtagca atggtgatga atatgctttt
     2521 tcagttcaga aaactacaga tggaggtttt attgttgaaa gtaatatata ttctaataac
     2581 gcagatattt gggattatca tggaggaggt gatgcatggg ttgtaaaatt agaaaatacc
     2641 ggcaatatag aatggcaaaa atgtttgggt ggtagtgatg atgattatgc ttattccatt
     2701 cagcaaacta cggatggagg ctatattctg gcaggttata cagattctaa tgatgaagat
     2761 gtttctggaa atcatggagg gccgaatgat gcatgggttg taaaaattat tgaatcgaat
     2821 gtatctggcc tggtgtttca cgacacaaac cttaacggca ttcccgatga aggcgaacaa
     2881 ggtattgccg gacatttggt aaaactggaa ccgggtccgt tttatacctt tacaaacaac
     2941 gagggaaaat actttttcag ggctgaagca ggagataata tcgtaagcta tgtgccggta
     3001 aattactggt atgcaaccgc cgaagaagac taccagtttt cggtaatcag taatacccaa
     3061 atcatcgaca ctctagattt cggcatcgct acccgcgaaa actcgcccga tgtgtccgtt
     3121 tatctaaccg gctcacctac acgtgctggc tttgataccc attattggat tagctacaaa
     3181 aaccagggaa cgcttactac ttccggcacc gtgaatgtgg ctttcgaccc cttgctaaca
     3241 tttgtttcat caactgaagc tcccacttca caattaggca atgtctttac ctggaattac
     3301 ggccccatgg gtccgggagt tgagcaccaa ttccgtgttg attttgaggt tccggggatt
     3361 gattatcttg gtgatacgct gttttcccat gtatggatta ctccgcttga ccccgacacc
     3421 tgcttggtga ataattacga caccatttat caggaaatta ccggctcata cgac
//
LOCUS       DAOOQP010000197         3452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_32631_length_3452_cov_4.977039, whole genome shotgun sequence.
ACCESSION   DAOOQP010000197 DAOOQP010000000
VERSION     DAOOQP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32631_length_3452_cov_4.977039"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLK75_12690"
     CDS             complement(<1..844)
                     /locus_tag="PLK75_12690"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOY40147.1"
                     /translation="MYKNSTFNLFSKYIIRLLFFVSVFGMLSGNLYAISSESNNIFHK
                     NGGVCGAVDVPLWLDDTIVKDSITVHAPSNTIEHPVDYQSKDSCFVDLKNKRAYLYGE
                     AQVTYGDITLTAEYIMIDFADNTVFACGETDSAGSVFGKPVFVEGEETFKADTIRYNF
                     NSKKAIIKEVTTEFEGSFLHGGVTKKQADNEVHLIHGKFTTCNLDHPHYYFNISKAIM
                     IPDDKIVSGPAYLVIEDVPLPLGIPFGFFPNKKGSTSGIVFPEFGEEQNRGFFLRRGG
                     YYFAI"
     gene            897..2072
                     /locus_tag="PLK75_12695"
     CDS             897..2072
                     /locus_tag="PLK75_12695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOY40148.1"
                     /translation="MANIPKHVNIVFAFCLFFIMLALPYAKVSAQSAGSVSVKRVVID
                     PGHGGKDPGAVGAISKEKDLTLAISLKVGDLIKKSFPDVEVIYTRSTDEFVELHKRAQ
                     IANEKKADLFICIHVNAIAGGTAYGAETFVMGLSVTDENMRVAKLENSVILKEDNYQN
                     NYEGFDPNSPESNIMFSLYQNAFIEQSLKLSDKVQKYMKSVAGRYDRGVKQAGFLVLW
                     RTAMPSILIECGFITHKDEEKYMNSEEGQNQIAKAIFDAFSDYKKSLDNEGKEIKGSG
                     SQDSVTEKPENNTAIEKPISDNSFNFAEGIFFGVQVKASAKKIDISKDSFLSGKGLVY
                     EYEQGGLFKYVVAVSRDYESTNAEFVKLRGEVKDCFMVAFRNGERIPVIQAQKELKQ"
     gene            2076..>3452
                     /locus_tag="PLK75_12700"
     CDS             2076..>3452
                     /locus_tag="PLK75_12700"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOY40149.1"
                     /translation="MNKRRKYILFGFMAVFILAFSYWGFNYLKGADLFKKRNTFYAYY
                     NRIDGLGVGSPVMINGYKIGQVTKIDLLIEKGGILLVTLETMSDIQIPDSSVAEIFST
                     DLMGTKGIQFNLSDKTTYYNSGDTVIGTIEQSLKEQVSMQMLPLKSQAEDLMKEIENA
                     ITIITYIFNENTRQNLEKSFESIKTTLSYLESSSITLDTLLSSESSRLSRIFMHVEKI
                     TANLKDNNDKITNILTNLSQFSDTLVALNLGQTIYNANKAISDIAYITDKINRGEGTL
                     GMLVNNDTLYYNLEKASDDLDKLIVDLQRNPGKYVNFSLLHFGRSVSVINEEDLSKRD
                     QKYLDKQREKSRKEAQRNANTKKTTQTTNRSILDEPVEFDNNAPAYYMIQIIAGSNKL
                     NANSPEFKSYKNVKEIYNDGVYRYLLMPHTDATNTQKYLNLIKEDFEDAFPVGIYNGA
                     IVPYSQAIE"
BASE COUNT         1110 a          721 c          769 g          852 t
ORIGIN      
        1 tgatggcaaa gtagtagcct cccctgcgaa gaaagaaacc cctgttctgc tcttcgccga
       61 attcgggaaa cacaataccc gaagtagaac cctttttatt cggaaaaaaa ccgaacggaa
      121 tgcccagagg aaggggaaca tcttcgatta ccagataagc aggtcccgaa acaattttat
      181 cgtcgggtat catgatggct ttgctgatat tgaaatagta gtgcgggtgg tcgaggttgc
      241 aggtagtaaa ttttccgtga atcaaatgca cctcgttgtc tgcctgcttt ttggtaactc
      301 cgccgtgaag aaagctgcct tcgaattctg tggtaacttc tttgattatg gcttttttcg
      361 aattgaaatt gtagcgaata gtgtcggctt taaatgtttc ttccccttcg acaaacacag
      421 gcttgccaaa aacgcttccg gcagagtcgg tttcgccaca ggcgaatacg gtgttatcgg
      481 caaaatcgat cataatatat tctgctgtaa gcgtgatatc gccatacgtt acctgcgctt
      541 ctccatagag gtaagcacgt ttgtttttca ggtcaacaaa gcatgaatct ttcgactggt
      601 aatcgaccgg atgttcgata gtattcgatg gcgcatgaac cgtgatactg tctttcacaa
      661 tagtatcgtc gagccacagg ggcacatcaa ctgctccgca gacaccaccg ttcttatgaa
      721 agatattatt actttccgaa ctaatggcat aaagattgcc tgataacata ccgaaaaccg
      781 aaacaaaaaa taaaagccgt atgatatatt ttgagaacaa attgaaagtg ctatttttgt
      841 acaaatactt tttaaaatta atgcccaaaa ctaagcaaaa tttacactcg ttcaacatgg
      901 ctaacattcc gaaacatgta aatattgtat tcgctttctg tttatttttc ataatgctgg
      961 cattgccata cgcgaaagta tcagctcaga gcgccggtag cgtgagtgta aaaagggtgg
     1021 taattgaccc gggacacgga ggcaaagacc ccggtgccgt gggagctatt tccaaagaaa
     1081 aagacctgac gctagccatc tcacttaagg tgggcgatct gataaaaaag agtttccctg
     1141 atgtggaagt aatatacacc cgcagcaccg acgagtttgt tgaattgcat aaaagggctc
     1201 agattgccaa cgagaaaaaa gccgacctgt tcatctgcat acatgtgaat gccattgcag
     1261 ggggaaccgc ctatggtgca gaaacgtttg taatgggatt gagtgtaact gacgaaaaca
     1321 tgcgcgttgc taaacttgag aactccgtta ttcttaagga agacaactac cagaacaatt
     1381 acgaaggatt cgacccaaac agtcccgaat cgaacattat gttttcgctt tatcaaaatg
     1441 cctttatcga acaaagcctc aagctcagcg acaaagtaca gaaatatatg aagtcggttg
     1501 ccggacgtta cgaccgcggt gtgaaacaag ccgggttcct cgtgctttgg cgtactgcca
     1561 tgcccagcat acttattgaa tgcggtttta tcacccataa ggatgaagaa aaatacatga
     1621 acagcgaaga aggtcaaaac cagattgcaa aagcaatatt cgatgcgttc agcgactata
     1681 agaaaagcct cgataatgaa ggcaaggaga taaaaggatc cggctcgcag gattcggtta
     1741 cagagaaacc agaaaataat acggctatag aaaaacctat ttcagacaat tcttttaact
     1801 ttgcagaagg aatatttttt ggagtgcaag tgaaagcatc ggcaaaaaaa atagatattt
     1861 cgaaagacag cttcctgagc ggcaaaggat tggtatatga atacgagcag ggaggtttgt
     1921 tcaaatatgt ggttgccgtg agccgcgatt acgaaagcac caatgccgag tttgtaaagc
     1981 tgaggggtga agtgaaagac tgctttatgg tagctttcag aaatggggaa cgaatacctg
     2041 tgatacaggc gcaaaaggag ttaaaacagt aaactatgaa caaacgcagg aaatacatac
     2101 tattcggttt catggcagtg ttcatcctgg cattttcgta ctggggcttt aactatctga
     2161 aaggtgccga cctgtttaag aaacggaata ccttttatgc ctattataat cgcatcgacg
     2221 gtctgggcgt tggaagtccc gtaatgataa atgggtataa aatcggacaa gtcaccaaaa
     2281 ttgatttgct tattgaaaaa ggaggcatat tgctggttac cctcgaaacc atgagcgata
     2341 ttcagatacc cgattcgagt gtggctgaga ttttcagcac cgaccttatg ggtaccaaag
     2401 gcatacagtt taacctcagc gataaaacta cctactacaa ctcgggcgac acggtgatag
     2461 gcaccattga gcagagcctg aaggaacagg tgagtatgca gatgcttccg ctgaaaagtc
     2521 aggcagaaga cctgatgaaa gaaatagaaa atgccattac gattattacc tacattttta
     2581 atgaaaatac aaggcagaat cttgaaaaaa gtttcgaaag cataaaaact accttatctt
     2641 atctggagtc gtcgtccatc accctcgaca ccctactttc gtcggaaagt tcacgtttat
     2701 cgcggatttt tatgcatgtc gaaaaaatta ccgccaacct gaaagacaac aacgataaaa
     2761 ttaccaatat actgacgaat ttgtcgcagt ttagcgacac actggtagcc ttgaatctgg
     2821 ggcagaccat atacaatgcc aacaaagcca tcagcgatat tgcctacatc accgataaaa
     2881 taaatcgcgg agaaggaaca ttggggatgc ttgtaaacaa cgatacactg tactataatc
     2941 tggaaaaagc cagcgacgat ctcgacaaac tcattgttga cctgcagcgc aacccgggta
     3001 aatatgtaaa tttcagtttg ctgcacttcg gacgcagtgt gagtgttatc aacgaagaag
     3061 acctgagcaa gcgcgatcag aaatacctcg acaagcaacg ggagaagagc cggaaagaag
     3121 cccagcgcaa tgcgaacacg aagaaaacca cacaaacaac aaaccggagc attcttgatg
     3181 agccggtgga attcgacaat aatgcacctg cctactacat gattcagata attgcaggct
     3241 ccaataagtt gaatgccaat tcacccgaat tcaaatcgta taaaaacgta aaagaaattt
     3301 acaacgatgg ggtgtatcgt taccttctca tgccacatac cgatgccacg aacacccaga
     3361 agtatctcaa cctgattaag gaagattttg aggatgcatt cccggtcggc atttataatg
     3421 gcgctattgt gccttattcg caggcaatag ag
//
LOCUS       DAOOQP010000198         3449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_32666_length_3449_cov_5.642899, whole genome shotgun sequence.
ACCESSION   DAOOQP010000198 DAOOQP010000000
VERSION     DAOOQP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32666_length_3449_cov_5.642899"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1550)
                     /locus_tag="PLK75_12705"
     CDS             complement(111..1550)
                     /locus_tag="PLK75_12705"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOY40150.1"
                     /translation="MKKIILPLAFCLLSMLVFSQGYEIKLKVNGVHDTVLYLGHHFGD
                     KKYVIDTARIDSKGNCTFAGKEKLNHGIYIVVMPSLKMTYFEIIVGDEQHFSIETDTA
                     RYVDNMKIKGSAENKLFNDYQRKMAELQSKQLDYINLKSKYSDMPDSVKLYNEKIRDI
                     SEQRHQYMLNMVDKNSNTFFAKVLKSMIEIDVPDYPRDQNGNITDSTFQWRYYKDHYF
                     DNIDFNEAGLLRTPVFQGKLTYFFDKVVVPLPDSMKFESDRVIKMAYEGDTLMFRFVA
                     AHLLNYFETSKIMGFDEVFVHIAEQWYLSGKAYWGDSTFMAKLTERVLKITPNIIGNV
                     APDLKKMETYDETYMSLHAVDAEYTILVFWEPDCGHCKKEVPELFEKWRDTLSNMNVK
                     VFCLYTQYEKEKWVDFINEKDMIGSGWYNVWDGPYPHSNFRNFYDIYSTPVVYMLDRE
                     KRIVAKRIGVDSITEFIRFDKQRRAAEKE"
     gene            complement(1649..>3449)
                     /locus_tag="PLK75_12710"
     CDS             complement(1649..>3449)
                     /locus_tag="PLK75_12710"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOY40151.1"
                     /translation="YFAINDRVDLLLTGDIYSKGSFGLYARTNYKKRYKMNGSVDLSF
                     NNNRFGYRGLDNFRIDRLYSIKWKHNQDAKARPNSTFSADVNISSSAYDKWNSYNDAS
                     YMNTTKQSRISYSKVWPNTPFSLSMALAHTQNSTDSTVNLTLPDVTFNMSKIYPLKRK
                     SAVGKQRWYEKIGISYSAVLTNQATVREDSLLNITLDNLNNGIQHSIPVNTSLKVLKF
                     INITPAFQYTERWYSRSVEKLWQPDSVGSLTGDLVKNYTYGFNRAWDYKASCTMNTTV
                     YGMFQFKKGPVKAFRHVLTPSVSFIYYPDFGQPRYGYYDDYYRIQYNNNTGQYDTVSY
                     VYSVFEGLPYGTPTRGGAGSLSFNLGNTLDMKVRNRADTVSGEKKIKLLEGLSFNTNY
                     NIMADSLKWAPITMSGRTSLGKVSINFSSVFDPYAITRNEYGTPVRYNASEFSSSGKI
                     LRITSANLSVGFNFNAQSKTSKNQTAASQLYGYPDDYVDFDVPWNLRLSYNLRYSKPY
                     FDQTVTQSLQVSGDVNVTSKWKVSVSSGWDFVNDKITYTTINIYRDLHCWEASLQLIP
                     FGLHKSYSFKINVKAAMLQDLKLSKRRSWYDNF"
BASE COUNT          949 a          732 c          724 g         1044 t
ORIGIN      
        1 cggaaggcaa aggccgtcat cgaaaacgtc atgcaggcca ttactgacaa tgcaaacagt
       61 aataacttcc gacaatattt acgaggcatt tctaccatac aatgaagtag ttattctttt
      121 tctgctgcac ggcgttgttt atcgaaacga atgaattccg taatactgtc gaccccgatt
      181 cgttttgcaa caatgcgttt ctcacggtcg agcatataca ccacaggggt agaatagata
      241 tcgtaaaaat ttctgaaatt agaatgcgga tatgggccat cccacacgtt ataccaacca
      301 ctgccaatca tatctttttc attgataaaa tcaacccatt tttccttttc gtactgggtg
      361 tataaacaaa agaccttaac attcatgttc gacaatgtat cgcgccattt ttcgaacagt
      421 tcgggcacct cttttttgca atgtccgcaa tcaggttccc agaacaccag aatggtatat
      481 tcggcatcaa cagcatgcag cgacatataa gtttcgtcgt atgtttccat tttttttagg
      541 tcaggagcca cattgccaat gatattcgga gtgattttca acaccctttc ggtaagtttg
      601 gccataaatg tactgtcgcc ccaataggct tttccactaa ggtaccattg ttcggcaatg
      661 tgaacaaaaa cctcatcgaa acccataatt tttgaagttt cgaaatagtt gagcaggtgg
      721 gccgctacaa aacggaacat cagcgtgtcg ccttcataag ccatttttat aaccctgtct
      781 gattcgaatt tcatagaatc gggcaaaggg accacgactt tatcgaagaa ataggttaat
      841 tttccctgaa aaacgggggt tctcaacaaa ccggcttcgt tgaaatcgat attatcgaaa
      901 tagtgatctt tataatatcg ccattggaaa gtgctgtcgg ttatattacc gttttggtcg
      961 cgcgggtaat cgggcacatc aatctcaatc atcgatttca gtacttttgc aaaaaaagta
     1021 ttgctgtttt tatcaaccat attcaacata tactgatgcc tttgctcgct gatatctctt
     1081 attttttcgt tgtacaattt cacggaatca ggcatatcgc tgtatttgga ttttaggttg
     1141 atataatcga gttgtttgga ctgcaattct gccatttttc gctgataatc gttaaatagt
     1201 ttgttttcgg cagagccttt gattttcata ttatccacat acctggccgt atcggtttca
     1261 attgaaaaat gttgttcatc tcctacaatt atttcgaagt aggtcatttt taaactgggc
     1321 atcaccacga tataaatgcc atgattcagt ttttcttttc cggcaaaagt gcaattccct
     1381 ttcgaatcga tgcgggctgt gtcgattaca tattttttat cgccaaaatg atgcccgaga
     1441 tacaaaacag tatcgtgaac cccgtttact tttaacttaa tctcgtaacc ctgcgaaaat
     1501 accagcattg atagtaagca aaaggcaagc ggcaaaataa tctttttcat atcggatagg
     1561 ttttcaaaat ttcaaaatta ttaatagaaa cgcataaaat aataaaaggt atcgttaaaa
     1621 aacatttaaa ttttcaaaat gtgtgccatt aaaaattatc gtaccagctt cggcgcttcg
     1681 atagtttcag gtcctgcaac atagccgcct tcacgttaat tttaaagctg tatgatttgt
     1741 gcagaccaaa gggaatgagc tgcaaactgg cttcccagca atgcaggtcg cggtatatat
     1801 taatggtggt ataggtgatt ttgtcattga caaaatccca tccactcgaa acagatactt
     1861 tccattttga agtaacgttc acatcacccg atacctgaag gctttgtgta accgtctggt
     1921 cgaaatatgg cttggagtag cgcaggttat agcttagtcg caggttccat ggaacgtcga
     1981 aatcgacata atcgtcgggg tagccgtaaa gttgagaagc cgctgtctga tttttagagg
     2041 ttttactctg tgcattgaaa ttgaaaccaa cgctcaggtt tgcactggta attctcaaaa
     2101 ttttacccga agaggaaaat tccgaagcat tgtagcgtac aggagtacca tattcgttac
     2161 gggtaatagc atacgggtcg aacaccgaag agaaattaat gctgactttt cccagactgg
     2221 ttctgcccga catggtaatg ggtgcccatt tgagcgaatc ggccatgata ttataattcg
     2281 tattgaacga caagccttcc aacagtttaa ttttcttttc accagaaaca gtatcggcac
     2341 gattgcgaac cttcatatcg agtgtatttc ccagattaaa cgacagcgac ccagctcctc
     2401 ccctggttgg tgttccgtaa ggcaagcctt cgaacactga atacacataa ctcacagtgt
     2461 cgtactgccc cgtgttgttg ttgtactgaa tccggtaata gtcgtcgtag tagccatagc
     2521 gcggctgacc aaaatcggga taatatataa aacttaccga aggggtaagc acatggcgaa
     2581 atgctttcac cggtcccttt ttaaactgaa acatcccgta aaccgtggtg ttcattgtgc
     2641 atgaggcttt gtagtcccat gccctgttaa aaccataagt atagtttttc accaggtcac
     2701 ccgtaagtga acctaccgaa tcgggctgcc ataatttttc gaccgaccga gagtaccacc
     2761 gttcggtata ttgaaacgcc ggcgtaatgt tgataaattt cagtactttc agcgaggtat
     2821 tcaccggaat gctgtgctga ataccgttgt tcagattatc gagtgtgatg ttcagcagcg
     2881 aatcttcgcg aacagtggcc tgattggtaa gcacagccga gtagctgatg cctattttct
     2941 cgtaccaacg ttgctttcct actgccgact tacgtttgag cgggtaaatt ttgctcatgt
     3001 taaaagtaac gtcgggcaag gtcaggttaa ccgtactgtc ggtgctgttc tgggtgtggg
     3061 caagtgccat cgacaggctg aagggtgtgt ttggccacac tttactatag ctgatgcgcg
     3121 actgttttgt ggtgttcata tacgaagcgt cgttatacga attccatttg tcgtatgcgc
     3181 tggaactgat gtttacatct gccgaaaaag tactgttcgg acgggctttt gcatcctgat
     3241 tgtgtttcca tttaatgctg tacaaacggt cgattctgaa gttatcgaga ccacggtacc
     3301 caaagcggtt gttgttgaag cttaggtcaa cgcttccgtt cattttgtat ctctttttgt
     3361 agttggtgcg ggcatacagt ccaaaactcc ctttactgta aatatcgccg gtaaggagca
     3421 aatcgacacg gtcgttgatg gcaaagtag
//
LOCUS       DAOOQP010000199         3300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_34673_length_3300_cov_4.378120, whole genome shotgun sequence.
ACCESSION   DAOOQP010000199 DAOOQP010000000
VERSION     DAOOQP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34673_length_3300_cov_4.378120"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="PLK75_12715"
     CDS             complement(<1..1686)
                     /locus_tag="PLK75_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY40152.1"
                     /translation="MKKFWVLLVFLNFLVASSVLAQTVDRLCVDGRIYFKLKNEVPVN
                     VMHNNGIINPKDAYFLEDLIDKYKITDLVLPFISAESDILQRTFRMDFDNADMVDQLI
                     KELSQHPDIEYAEKAPLFFISLVPNDPYYNVSLSGGMWGSANSSWHLNLINASQAWDV
                     TPGDPDIVVAVLDNAIWIDHPDLANKVVSSIDLANGDNDPNPPDATYIWSHGTHSAGL
                     IAAETNNGIGVASIGNGISIMAVKLGDDASDGQSMAAGFEGIVWAADNGADVINMSWG
                     TPQFFQTMQNTVNYAYNKGCVLVGAAGNNGNGMETQVNPDIPINYVGYPAALPHVIAV
                     GSCDVGDNKSDFSNYGTWIDVLAPGGYASSGFLGIGAFAVLSTTYNTAGTVMDMLNGT
                     TGGAATFGVSGNYDLMQGTSMAAPVTSGLCGLMLSANPDLTPEELTFLLKSTCTNVNA
                     QNAEFIDSIGAGRINAYAAVVAASNAIAPLVADFTASSVSIPEGGSIDFTDLSIGTPV
                     SWEWAFEGAENPISNVQNPTNIVYNTAGVFQVSLTVTDAESNEDTEIKTYYIIV"
     gene            complement(1790..2464)
                     /gene="mnmD"
                     /locus_tag="PLK75_12720"
     CDS             complement(1790..2464)
                     /gene="mnmD"
                     /locus_tag="PLK75_12720"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOY40153.1"
                     /translation="MKLNTNTVFISDDGSKSVYNRLIDESFHSHSGALTESAYVYVFQ
                     GFSKFKSSVVNLLEIGYGTGLNAMLTYKANLLQRNIVQYHGVDTEILSDDILKALKSN
                     ASMLQIDNEYEFFVEHWNVASQIDDNFMLYKHKCSIHDFCLEEPFDLIYHDAFSPEKQ
                     PDMWSEELFQKLFTWLRPGGTLVTYSSKGIVKERLRSAGFVVKRLKGLPPKRHMLCAF
                     KPITPE"
     gene            2549..>3300
                     /locus_tag="PLK75_12725"
     CDS             2549..>3300
                     /locus_tag="PLK75_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40154.1"
                     /translation="MKNIFLTTLFVIATSTLWNTSVFSQISEGGTPPSFSELKSFEEI
                     DLVTIASPDLKDIQLLDDENEKNGTFYKISKLLDVDLNMANSGTWSTLQDGTEIWRLI
                     IESTDAKGLALHFDYFNIPVGAKFFVYNSDHSVVLGAFTSFNNTGNNATTIGILPGDE
                     MILEYVSPRAYSKSIGTSSVSEPDFQIASISYLFRGMEYFDPHPKSTGFGASDDCQVN
                     INCSEGTNWQTQKKGVARIYVVEDGYGGYCSGT"
BASE COUNT          994 a          705 c          644 g          957 t
ORIGIN      
        1 tacaataata tagtaagttt taatctctgt gtcttcattt gattcggcat cggtaacagt
       61 taaacttacc tgaaaaacac cggcagtatt gtaaacaatg ttagttgggt tctgcacatt
      121 gctgataggg ttttctgctc cttcaaaagc ccattcccat gataccggag taccaatact
      181 caaatcagta aaatcgatgc ttccaccttc cgggattgaa acagaagacg cggtaaaatc
      241 agccacaaga ggggctatag catttgacgc tgcaactaca gcagcatagg cattgattct
      301 tccagctccg atactatcga taaattctgc attttgagca ttcacatttg tacaagtact
      361 cttcaatagg aaagtaagtt cttccggagt taggtcgggg tttgccgaca acattaatcc
      421 gcataagcct gaagtaacgg gggctgccat tgaagtaccc tgcatcaggt cgtaatttcc
      481 tgaaacacca aatgtggctg caccaccggt tgtaccgtta agcatatcca tcaccgtacc
      541 ggcggtatta taggttgtac tgagaacagc aaaagcacca ataccaagaa agccacttga
      601 tgcatacccc ccgggggcca acacatcaat ccatgttccg tagttcgaga aatctgattt
      661 attatcgcca acatcgcacg aaccaacagc aattacatga ggtagggcag cggggtatcc
      721 cacatagttg ataggtatat ctgggttaac ctgagtttcc attccgtttc cattgttccc
      781 ggcagcacca acgagtacgc agcctttgtt gtaggcataa ttcacggtgt tttgcatggt
      841 ctgaaaaaac tgaggtgtgc cccacgacat attaataaca tcagcaccgt tatcggcagc
      901 ccaaacaata ccttcgaaac cggcagccat cgactgacca tcgctggcat catcacccag
      961 ttttactgcc ataatactga taccgttgcc tatggaggct acgccaatgc cattgttggt
     1021 ttcagcggct ataagtcctg ctgagtgtgt tccgtgcgac caaatataag tcgcatccgg
     1081 tggatttggg tcgttatcgc catttgcaag gtcgattgat gaaacaactt tatttgccaa
     1141 atcgggatgg tcgatccaaa tcgcattatc aagcacagca accacaatgt cgggatctcc
     1201 aggggttaca tcccaggcct gactggcatt aatcaggttt aggtgccacg atgaattggc
     1261 agagccccac attcctccac ttaacgaaac attgtagtat ggatcgttag gcacaagtga
     1321 gataaaaaac agaggagctt tttcggcata ctcaatgtcg ggatgttgcg ataattcttt
     1381 tatcaactga tctaccatgt cagcattgtc aaaatccata cggaacgtgc gctgtagtat
     1441 atcgctttca gcactgataa aaggcaacac caaatcagta atcttgtact tgtcgataag
     1501 gtcctcaaga aaatacgcat ccttagggtt aattatcccg ttgttgtgca taacatttac
     1561 cggaacctca tttttaagtt taaaataaat ccttccgtca acacacaaac ggtctactgt
     1621 ttgtgctaaa acgctacttg caaccagaaa attgaggaat acaagtaaaa cccaaaactt
     1681 tttcataata ctgttttttt attgttatta gttatttcta tacctcatac aaggcttcaa
     1741 atttacaaaa gtttgaatta tttttaaaaa aaatctttaa ctccttccac tattctggtg
     1801 ttattggttt aaatgcgcac agcatatgcc gtttaggggg taatcctttg aggcgcttca
     1861 caacaaagcc ggcacttcgc agcctttcct tgacaattcc cttggaggaa tatgtaacca
     1921 acgttccacc cggtcgcaac catgtaaaca gtttctgaaa aagctcttca ctccacatat
     1981 ccggttgctt ctcaggcgaa aacgcatcgt ggtatatcag atcaaatggt tcttcaaggc
     2041 aaaaatcatg aatactgcac ttatgcttgt ataacataaa attgtcgtcg atttgagatg
     2101 caacattcca atgttcaaca aaaaactcat attcattatc gatttgaagc attgaagcat
     2161 tgctcttcaa agccttgaga atatcatcag aaagaatttc tgtatctaca ccatgatatt
     2221 gcactatatt cctctgtaat aagtttgctt tataagtaag catagcattc aaacctgttc
     2281 cgtagccaat ctccagtaaa ttgaccacac tactcttaaa ttttgaaaag ccctgaaaaa
     2341 cataaacata ggccgattct gtcagagcac cactatggct gtgaaaactt tcatcaatca
     2401 aacggttata aacacttttt gacccgtcat ctgaaataaa cactgtgttt gtattaagtt
     2461 tcatagtatt gcaataattt ttttttgttg cctgtaaatg tatgtggaaa tttaataatt
     2521 tagctcagta aaatattaat taaaatttat gaaaaacatt tttttaacaa cgttatttgt
     2581 tatagcaaca tcaactctat ggaatacttc tgtattttca cagatttccg aaggaggtac
     2641 gccacccagc ttcagcgaat taaaatcgtt cgaggagatt gacctggtga ccattgcttc
     2701 accagattta aaagatattc agttactcga cgatgagaat gagaaaaatg gaacatttta
     2761 caaaatttcc aagttattgg atgtagattt gaatatggct aattcgggta catggagtac
     2821 gcttcaggat ggcactgaga tatggcgact gataattgaa agtaccgatg caaaaggatt
     2881 agccctgcat ttcgattatt tcaacatacc tgttggcgca aaattctttg tttataacag
     2941 cgatcacagt gttgtgctgg gggccttcac ttctttcaat aatactggta ataatgcaac
     3001 aaccattggg attctaccgg gtgatgaaat gattctggaa tacgtttctc cgcgtgcata
     3061 ttctaaatcg ataggtacaa gcagtgtttc agaacctgat tttcagattg caagcatatc
     3121 gtacttattc agagggatgg aatatttcga tccacacccc aaaagcacag ggtttggagc
     3181 gtctgacgat tgtcaggtta acatcaactg ctccgagggt accaactggc agacacagaa
     3241 aaagggggtt gcacgcatat atgtagttga agacgggtat ggaggttatt gttccggaac
//
LOCUS       DAOOQP010000200         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_35099_length_3270_cov_4.371695, whole genome shotgun sequence.
ACCESSION   DAOOQP010000200 DAOOQP010000000
VERSION     DAOOQP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35099_length_3270_cov_4.371695"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..2660
                     /locus_tag="PLK75_12730"
     CDS             168..2660
                     /locus_tag="PLK75_12730"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOY40155.1"
                     /translation="MQNKADSSSETSLFSVNEEKLTPGALSGAITEKLFYHLGRRPHN
                     ASMNDFYLAVSITVRDRLMERALLTADNAFSQKNQKIVCYLSAEFLMGPQLGANLIDL
                     GIEANMIKALKTFNISLEDVIAMEEEPGLGNGGLGRLASCYMDSLASVEIPAVGYGIR
                     YEFGIFDQEIRDGWQVEVTDKWLKNGNPWEIPRPDFAVYVNMGGYTEHTTDTNGVLRV
                     AWIPDNVIKGIPYDTPELGYKVQSCNFLRLWRSEAVESFDFSAFNHGDYFRAVNEKML
                     SENISKVLYPNDESSQGKELRLKQQYFFVSCSLQDMMRMHIEAGGNPAGFHKRYSVQL
                     NDTHPSIAVAELMRLLIDTYELDWETAWQVTTSTMCYTNHTLLPEALEKWALELFQKL
                     LPRHLEIIYEINHRFLMEVKQRFPGDICKLARLSIIDENKGKFVRMANLACAGSSSIN
                     GVAELHTRLLKTTVLPDWVDMYPDKFHNITNGVTPRRFIVLSNPGLTNLINDTIGNEW
                     PSNLNELRKLENFITDTSFLNRWQTVKTENKKYLAKLIKSKTGVSVNPESMFDIHVKR
                     IHEYKRQHLNILNILTRYIRIKSNTAINEAPRTYIFGGKAAPGYYMAKLMIKLINSVA
                     DLINNDTRINEFIKVVFYPDFNVTNAQPIYPAADLSEQISTAGKEASGTGNMKFAMNG
                     ALTIGTLDGANIEIREEVGHDNFFLFGLTAEQINETMKKGYDPSFIYRSNEELCEVIG
                     IMESGLLSSGDKELFKPLVQTLVSYDTFMLLADYPLYVACQDYVNQVWSNKNLWATMS
                     VRNVARIGKFSSDRSIREYCEKIWHVKPCSIQ"
     gene            complement(2668..2814)
                     /locus_tag="PLK75_12735"
     CDS             complement(2668..2814)
                     /locus_tag="PLK75_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40156.1"
                     /translation="MLGHKVYYGDYSYSGGNSITLINLSNVTPTIFMVRIQGMVLLEV
                     SDLL"
     gene            complement(2857..>3270)
                     /locus_tag="PLK75_12740"
     CDS             complement(2857..>3270)
                     /locus_tag="PLK75_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40157.1"
                     /translation="NYKVKIISSGEHCNAKFSDVACITVVNNTGKAAADNYNPSESET
                     IQQNNDGLFKRSEISLFPNPTNEIINIKALVEQEGDYCIEVLDILGFSVYMGTFVSSD
                     GEVSVSINLNQYPSAMYVVRITGKDTKLVARFIKN"
BASE COUNT         1039 a          665 c          633 g          933 t
ORIGIN      
        1 tttacaataa agatgaagaa acctatatcc tcagcaagga gtaggatttt ataaaggttt
       61 tatctaatta tttactttcc taaataatta taaggcaacc tactcagttg gggttcatat
      121 aagaagttat caactttttc gtatctttac ataaaatcct cagtaagatg caaaacaaag
      181 cagattcatc gtctgaaaca tcacttttca gcgttaatga agaaaaactt acccccggag
      241 cacttagtgg agcaatcacc gaaaaactgt tttatcattt gggtagaaga cctcacaatg
      301 cttcaatgaa cgatttttat cttgccgtat cgattacagt aagagaccgt ttaatggaac
      361 gtgctttact aacagcagac aatgctttct cccaaaaaaa tcaaaaaatc gtatgctacc
      421 tttccgcaga attcctaatg ggacctcagt tgggtgcaaa tctgattgat ctgggaattg
      481 aagcaaacat gataaaagca ttaaaaacat tcaatatcag tttggaagat gtaatagcca
      541 tggaagaaga acctggcctt ggtaatggcg gactgggcag actggcttca tgttatatgg
      601 attcacttgc ttccgtcgaa attcctgcgg ttggatacgg aattcgctat gagttcggca
      661 tttttgatca ggagatccga gacggctggc aggtcgaagt tacagataag tggcttaaaa
      721 acggaaatcc atgggaaata ccacgacccg attttgctgt atatgttaat atgggaggtt
      781 atactgaaca tacaaccgat acaaacgggg tacttcgagt agcatggatt cccgataatg
      841 ttataaaagg tattccctat gacacccctg agctaggtta taaagtgcaa tcgtgcaatt
      901 ttctgcgtct gtggcgatca gaagcagtag agtcgttcga cttttcagct tttaatcacg
      961 gtgactattt cagggctgtt aacgaaaaaa tgctttccga aaacattagc aaagtacttt
     1021 atccgaatga tgagtcaagt cagggtaaag agcttcggtt aaaacagcaa tatttttttg
     1081 tatcttgctc tctacaggat atgatgcgga tgcacattga agcagggggt aacccggcag
     1141 gttttcataa acgatactct gttcaactga atgacaccca tccttcgata gcagttgcag
     1201 agttgatgcg cttacttatc gatacgtacg agctcgactg ggagacagcc tggcaagtaa
     1261 ctacaagtac tatgtgttat acaaaccata ctctgttacc cgaagcactt gaaaaatggg
     1321 cgctagaact atttcagaaa ctacttccgc gccacttgga aattatatat gaaatcaacc
     1381 accgtttttt gatggaagtt aagcaacgtt ttccgggtga tatttgtaag cttgcacgcc
     1441 tttcgattat tgatgaaaac aagggaaaat tcgtcaggat ggcaaacctc gcatgcgcag
     1501 gcagctcttc aatcaatggc gttgctgagt tacacactcg tttgctaaaa accacagttt
     1561 tgccagattg ggtagatatg tatcccgata agtttcacaa tattacaaat ggtgttactc
     1621 cgcgccgatt catagtattg agcaatccgg gacttacaaa ccttattaac gacactatag
     1681 ggaatgaatg gccgagcaac ctgaatgaac tacgaaaact cgagaacttt atcacggaca
     1741 cttcatttct aaaccgttgg caaaccgtta aaaccgaaaa caagaagtat ttggcaaagc
     1801 taataaaaag taaaaccggc gtttcagtca atcctgaatc aatgttcgac attcatgtaa
     1861 agcgcattca tgagtacaaa cgccagcact tgaatattct taacatactt acacggtata
     1921 tccgcataaa aagtaatact gctattaatg aagcaccacg cacgtatata tttggaggta
     1981 aggctgctcc cggctactat atggcaaaac tgatgataaa actcatcaac tctgttgcag
     2041 acctaattaa taacgatacc cgaataaacg aatttataaa agttgttttt taccctgatt
     2101 tcaatgtcac caatgctcaa cccatttacc cggcagcgga tctgtcagag caaatatcta
     2161 ccgccgggaa agaagccagt ggaaccggaa acatgaaatt cgcaatgaac ggtgcattaa
     2221 ccattggtac cctcgacggt gctaacattg aaatacgtga agaagtgggt cacgacaact
     2281 ttttcctctt cggactaaca gccgagcaaa taaatgaaac aatgaaaaag ggctatgacc
     2341 ccagctttat ttatcgtagc aacgaagaac tttgtgaagt aatagggatc atggagtcgg
     2401 gattactctc cagtggagac aaagaacttt ttaaaccatt ggtacaaaca cttgtctcgt
     2461 acgacacatt tatgcttttg gccgattatc cgttgtacgt tgcctgtcag gattatgtta
     2521 atcaggtctg gagcaataaa aatctatggg caacgatgtc ggttagaaat gttgcccgaa
     2581 taggtaagtt ttcctccgat cgttcaatac gtgagtattg cgaaaaaata tggcacgtaa
     2641 aaccatgcag cattcaatag ccttgtttta caacaagtcg cttacttcta gtaacaccat
     2701 tccctgtata cggaccataa aaattgtagg agtcacattg cttaagttta tcagcgtaat
     2761 ggagttacca ccggaataac tgtagtcgcc ataatatacc ttgtgcccta gcatactatt
     2821 tcattatcat atttttacca cattatcaat aacaccttag ttttttataa aacgtgcaac
     2881 cagtttggta tcctttccag taattctaac aacatacatt gccgatggat actgatttag
     2941 gtttattgaa acacttacct ctccatcaga agatacaaaa gtacccatat atactgaaaa
     3001 accaagaata tcaagcacct caatacagta gtccccctcc tgctcaacca aggcttttat
     3061 attgattatt tcgttcgtag gattggggaa aagacttatt tcgcttcgtt tgaacaaacc
     3121 atcgttattt tgctgaattg tttcactttc cgatggatta taattgtcgg ctgctgcttt
     3181 cccagtatta ttaacaacag tgatacaagc aacatcgcta aatttcgcat tgcaatgttc
     3241 tcccgacgaa ataattttaa ctttgtaatt
//
LOCUS       DAOOQP010000201         3116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_37453_length_3116_cov_4.596864, whole genome shotgun sequence.
ACCESSION   DAOOQP010000201 DAOOQP010000000
VERSION     DAOOQP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37453_length_3116_cov_4.596864"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..517)
                     /locus_tag="PLK75_12745"
     CDS             complement(56..517)
                     /locus_tag="PLK75_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40158.1"
                     /translation="MNSFAQPLGSRLFISVDGLAPALKFGFEKVISDRLSLKTSLGAC
                     IVGPGLLSYNLYGTYIITRPEKTFGCTLHFGFPDNYIDVLTPMFSLGLGGGAGIYYRF
                     KNYSVLTFRLGIISGPSVDNGKYAILTLPNYGIEYAFKFRNKNSSKHTVRD"
     gene            975..1772
                     /locus_tag="PLK75_12750"
     CDS             975..1772
                     /locus_tag="PLK75_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY40159.1"
                     /translation="MKIALIADIHEDVVSLKDALRIAENETCDHIVCLGDIIGYPYRR
                     AAYNHTRNASECISLVKNNCSCVVKGNHDIFHTSSFPKHATGFRFPKEWYRLSDDEKL
                     SLSNGRVWHYSDDSSLYLSDSEIEYLNSLPEFEVHNCNGLHLSFSHYLYPNITGYESS
                     LKGVNADVKTHFLHMATHNSPIGFVGHIHPEGVGICYETNGNITSAWFGRFRFFTYGS
                     RNIRDYRCVVGLPALADNGRENGFSVFDTVNRSLNTISLNPNRRAHL"
     gene            1789..>3116
                     /locus_tag="PLK75_12755"
     CDS             1789..>3116
                     /locus_tag="PLK75_12755"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOY40160.1"
                     /translation="MIKLKTLIKNKFFQKIVVPATIALLLFIVTVFAFILPAFERNAI
                     NQKKNMLKELTSTAWSILDKYNKEYSTGKLGLEAAQSLAKEDIEALRYGTQKKDYFWI
                     TDMEPNMLMHPYMHELTGTPLQDYTDPDGKKLFIEAVEIAKKQGEGFISYKWQFMDDS
                     SRIVAKLSYVKKFEPWKWIIGTGIYLDDVQTEIARLRNQLLIILLLSTLLIATFIAFA
                     TWQSLLLENKRLMAEEQLRISQERYRALVEASSEGALLVLHSYIYANKTLLNMLDYDE
                     YEIEGFTVWDIIASRELQEKYSDFETLYNATDLSESFESFVTRKNQLKMRVMVNMSRI
                     TMAGNKALVVLIKEIPVTGHSDADSVPLNGESLVTFDFGNFGFFRSSFSRNSRFTEAT
                     QPAVKILGFRSKEEMMKTRIDDLFANKSELKSFTLQLLKYGFVNNKVLRL"
BASE COUNT          961 a          614 c          628 g          913 t
ORIGIN      
        1 taccttttga ctcattatac aatctctttt ccccggtact tcatttccta tcacatcaat
       61 cgcgtactgt gtgtttactg ctgtttttgt ttctgaactt aaatgcgtat tcaataccat
      121 aattgggaag agtcaatatg gcatatttac cattatcaac cgaagggcct gagattatcc
      181 ccaaccgaaa tgttaaaacg gagtagtttt taaaccggta atatattccg gcaccgcctc
      241 ccaatcccaa cgaaaacatc ggggtaagca catcgatata attgtcggga aacccaaagt
      301 ggagggtgca accaaaagtt ttctctggtc gcgtaataat gtaggtgccg tataaattgt
      361 aagataacaa accgggtccc acaatacatg ccccaagcga tgttttcagc gataaacggt
      421 ccgaaattac tttttcaaaa ccaaatttca gggctggagc aagtccgtca acagatatga
      481 acaatcggct tccaagaggc tgtgcgaaac tgttcatagt gaaaattgca ataaatacac
      541 aaagcaaggt tttctttaat cgttttatat tcattggttc accttgttat atcaatcact
      601 gcatataatt tatcgttttc aaaatgtgaa cattatttgt attcaaattt atctccgaat
      661 taaatcattc aacatcgaac ttataaagca aatgtattgc atttagtttt tatttcaata
      721 aaatttttaa gactgtaatc tactgattgt agtatttgat tagtgcaaac atgtaaccgg
      781 ctacctgaat aaaatgcttt gaatttgctg ttgattggga atgaaattat tgatatcttt
      841 accctgacaa taatgcggaa gaagttgttg cgcagggtta cagaaaatga attttaataa
      901 tgagtattga ataagaagca tagctgcgac aacaataagg gacctgggtt ctaaatgaaa
      961 atacctgaaa aacattgaaa attgcattga ttgccgatat tcacgaagac gttgttagcc
     1021 tgaaagatgc gcttcgaatc gccgaaaacg aaacttgcga tcatatagta tgtctgggcg
     1081 atattattgg ttacccttat cggagagctg catataatca tacccgcaat gcctctgagt
     1141 gtatttctct ggtgaaaaat aattgtagct gtgttgttaa aggtaaccat gatattttcc
     1201 atacttcaag ctttccaaaa catgcaacag gtttccgatt ccccaaagaa tggtatcgtt
     1261 taagcgatga cgaaaagctt tcattatcaa acggtagggt atggcattat agcgacgatt
     1321 cttcgttgta tcttagcgat agcgaaatag aatatctcaa ttcgctgccc gaattcgaag
     1381 tgcataactg caatggttta catttgtcgt tttcgcatta tctgtacccc aatattacag
     1441 gatatgaatc ctctctcaaa ggggtaaatg ccgatgtgaa aacacatttt ttgcatatgg
     1501 ctacccacaa tagcccaatt ggttttgtgg ggcacattca tcccgaagga gtgggcatat
     1561 gttacgaaac caatggcaac attacttctg catggtttgg caggttcaga ttttttacct
     1621 atggcagcag aaatatccgc gattatcgtt gtgtggttgg tttgcccgca cttgccgata
     1681 atgggcggga aaacggattt tctgttttcg ataccgtcaa ccgcagcctc aacacaattt
     1741 cgctaaaccc caacagaagg gctcatttat aaatcaaaaa atggtgttat gataaaattg
     1801 aaaaccctga taaaaaataa attctttcag aaaatagtag ttccggctac aattgctttg
     1861 ctgcttttta ttgttacggt gtttgcattt atactgccgg catttgaacg caatgccatt
     1921 aatcagaaga aaaacatgct taaggagctc acttctacag catggagtat tctcgacaaa
     1981 tacaataaag aatacagcac cggtaagttg ggtctggaag ctgcgcaatc tcttgcaaaa
     2041 gaagatattg aagctttacg ttatggaact cagaaaaaag attatttctg gataaccgat
     2101 atggagccga atatgctgat gcatccctat atgcacgaac taacaggaac ccccttgcaa
     2161 gattacaccg acccggacgg aaaaaaattg ttcattgaag ctgttgaaat tgccaaaaaa
     2221 cagggtgagg gttttatcag ttataaatgg cagtttatgg atgacagcag ccgcattgtt
     2281 gcaaaactta gttatgttaa gaagtttgaa ccttggaaat ggatcattgg tacagggatt
     2341 tacctcgacg atgtgcagac cgaaatagca cggctgcgca atcaattgct cataattctg
     2401 ctgctatcaa ctttgctgat tgccacattt attgcctttg ccacttggca aagcctgctg
     2461 ctcgaaaaca aacgactcat ggccgaagaa caacttcgca tatcgcagga gcgataccgt
     2521 gcattggtcg aagcatcatc cgaaggagct ttgctggtgc tgcacagcta tatatatgcc
     2581 aacaaaaccc tgctcaacat gttggactac gatgaatacg aaatcgaagg ttttacggta
     2641 tgggatatta ttgcctcccg cgagcttcaa gaaaaatatt ccgacttcga gaccctatac
     2701 aatgccacag acttgtccga aagcttcgag tcgtttgtta cccgaaaaaa ccaactgaag
     2761 atgcgtgtaa tggttaatat gtcgcggata actatggccg ggaacaaagc cttggtagtg
     2821 ttaattaagg aaatccctgt gaccgggcat tctgatgccg attctgttcc gctcaatggc
     2881 gaaagtctcg ttacattcga ttttggtaac ttcgggttct tccgttcgag tttttcaaga
     2941 aacagtcgtt ttaccgaagc cactcaaccg gctgtgaaaa tcctgggttt caggtcgaaa
     3001 gaagaaatga tgaaaacccg tattgacgat ttgtttgcca ataaatcgga attaaaaagc
     3061 tttaccttgc aattactgaa atatggcttt gtaaacaata aagtactccg actgtt
//
LOCUS       DAOOQP010000202         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_37787_length_3096_cov_4.025978, whole genome shotgun sequence.
ACCESSION   DAOOQP010000202 DAOOQP010000000
VERSION     DAOOQP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37787_length_3096_cov_4.025978"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLK75_12760"
     CDS             complement(<1..1239)
                     /locus_tag="PLK75_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY40161.1"
                     /translation="MIELTQEEKIQIRQELDVILNQCVRCSGEENVKLITHAFELACK
                     AHEKMRRKSGEPYITHPIAVAKIVAGEMGLGTKSVVSALLHDVVEDTDYTIDDIRNLF
                     GDRIASIVDGLTKISNVLDKDASLQAENFRKMLLTISEDIRVVFIKLADRLHNMRTLD
                     SMPEYKQIKIAGETMYLYAPLAHRLGLYSVKTELEDLALKYEHPLIYNEIQKKLSDTA
                     EERDKYIGTFVAPLQKKLNTNKIICSINGRPKSVYSIWNKMQKKNVPFEEIYDVFAVR
                     IVFVPLDDISEKEQCWKIFSLITDIYVPRENRIRDWITTPKANGYEALHVTVMGPQGK
                     WVEVQIRSQRMDEIAEKGYAAHWKYKNGESTESQLDAWLETVKEHLKSSSSDALDFLD
                     DFKTNLFASEIYVFTPKGKLV"
     gene            complement(1307..2833)
                     /locus_tag="PLK75_12765"
     CDS             complement(1307..2833)
                     /locus_tag="PLK75_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY40162.1"
                     /translation="MKHYRNIIFLGFMLLFSYTTAYSQVDIPDAPRFLSASVTPGVLP
                     GEVTLKWNPSDSADVAGYIIYQVVGSTSVTIDTVRGRLSKQYINVNSQASVQTETYRI
                     AAFDTLLYKSMITDPHTTMIISSDYERCEQSVTLSWSEYVGFSEGVNAYRLFRRANDT
                     QYSSVAVLEDTILEYRDENLIPGKTYCYYVEALDSDGNSSTSNETCIYLDGFASPAYL
                     IASSTVVEGDALLLGFTVDTAAEVSQYKLLRSDQIDGNFSIIETQPANGLGTVNFIDN
                     NIDVNSTVFYYKLASFDPCGKLSGYSNICCNIVASVDGNNTLEHYISWTPYRDFINSV
                     DIYTINASYGNSSAFNVGQTDLNNLDFHYNIEYYINLMHAQQKTITNRYCYYIEAFEN
                     VGNGPVIVQGYSKSNLACTTHAPVFYMPTAFCPGSFIEENRVFRPVMSFIENGSYEFT
                     VYSKWGLTLFQTNDPFEAWDGYIDTMRAPENTYLYKVAYKDANGNEINHIGSFVLHYD
                     "
     gene            complement(2906..>3096)
                     /locus_tag="PLK75_12770"
     CDS             complement(2906..>3096)
                     /locus_tag="PLK75_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40163.1"
                     /translation="PEYRDYSVGNYIYFKNTGYFKRMGINTLKAKSYHYRHSNYLIKM
                     GFKQTFLDGELVFIKTLN"
BASE COUNT          871 a          648 c          639 g          938 t
ORIGIN      
        1 taccagtttt cctttaggtg taaacacata tatttctgat gcaaaaaggt tggttttgaa
       61 atcatcgagg aaatcgagag catcggatga agagcttttt aaatgttcct ttaccgtttc
      121 gagccatgca tcaagctgac tttcagttga ctctccgttt ttatacttcc agtgagcagc
      181 atagcctttt tctgctattt cgtccatgcg ttgggaacgg atctgcactt cgacccattt
      241 cccctgtgga cccatcactg taacgtgaag agcttcataa ccattggcct tgggggtggt
      301 aatccagtcg cggatacgat tttcgcgcgg aacataaata tccgtgataa gcgaaaatat
      361 cttccagcat tgctcctttt ccgaaatgtc atcgagtgga acaaaaacta tcctgacagc
      421 aaatacatcg tagatctctt cgaacggaac attctttttc tgcattttat tccatataga
      481 atacaccgat ttgggtcttc cgtttatgct gcagattatt ttattggtgt tgagtttttt
      541 ttgcagagga gctacaaaag taccaatgta cttatcgcgt tcttcagcgg tatcgctgag
      601 ctttttttga atttcgttgt aaatgagcgg atgttcatac ttcaacgcta ggtcttcgag
      661 ttcggttttt accgaataca acccaaggcg atgtgcgaga ggggcgtaaa ggtacatggt
      721 ttcgccggca atttttattt gcttgtattc cggcattgaa tcgagggtcc tcatgttgtg
      781 taacctgtcg gcaagtttga taaaaacaac gcgaatgtct tcggatatgg ttagcaacat
      841 tttcctgaag ttttccgcct gaagcgatgc gtctttgtcg aggacattcg atattttggt
      901 caacccgtca acaatacttg caatacgatc accaaaaagg tttcttatat cgtcgatggt
      961 atagtcggtg tcttcgacca catcgtgcaa caaggcactt accaccgatt ttgttccaag
     1021 tcccatctcg ccggcaacaa tttttgcgac tgcaatagga tgggtaatat aaggttctcc
     1081 cgatttccgc cgcatttttt cgtgagcctt acaggccaat tcaaaggcat gggtaatgag
     1141 tttcacattc tcctcaccgc tgcaacgaac acactgattg agaatgacat ccagttcctg
     1201 acgtatttgt attttctctt cctgagttaa ttcaatcatg aatacaaaat ttctccaaac
     1261 aaatttacaa tatatttggt aactctacta tgtaatacgg gcgattttaa tcgtaatgca
     1321 gaacaaaaga gccaatgtga ttaatctcat taccatttgc gtccttgtaa gctactttgt
     1381 acaagtatgt attttcaggt gctctcatag tgtcgatata accatcccag gcttcaaatg
     1441 gatcattggt ctgaaatagg gtcaaccccc attttgaata tacggtgaat tcataactgc
     1501 cgttttcgat aaatgacatc actgggcgaa aaaccctgtt ttcctcaata aagctaccgg
     1561 ggcagaaagc tgtcggcata taaaacacag gggcatgtgt ggtgcaggcg aggttcgatt
     1621 tgctgtatcc ctgcactatt acgggtccgt taccgacatt ttcgaaggct tcgatgtagt
     1681 agcagtaccg gttggtaata gttttttgct gagcatgcat caggttgata taatattcga
     1741 tgttgtaatg gaaatccaga ttattcaaat cggtctgacc cacattaaag gcacttgaat
     1801 ttccataaga agcattaatg gtgtaaatat cgacactgtt aataaaatca cggtaaggtg
     1861 tccaggatat ataatgctcc aatgtgttgt taccgtcgac agaagcaaca atgttgcagc
     1921 aaatatttga atatcctgaa agttttccgc aagggtcgaa tgaggccagt ttataataaa
     1981 atacagtaga atttacatca atgttgttgt cgataaaatt caccgttccc agcccatttg
     2041 ccggttgtgt ttcgataatg ctgaaattac catcaatctg atcggagcgc aacagcttgt
     2101 attgactcac ttcagcagcc gtatcaacag taaaccccag caataaagca tcgccttcaa
     2161 caaccgttga gcttgcaata aggtatgcag gtgatgcaaa cccgtcaaga taaatgcacg
     2221 tctcattcga tgtagatgag ttaccatcgg aatcgagagc ttccacatag tagcaatagg
     2281 tttttccagg tataaggttt tcatcgcgat attcaagaat tgtatcttcg agcacagcaa
     2341 ccgaagaata ttgtgtgtcg ttcgctcttc ggaagaggcg gtatgcattc acaccttcgc
     2401 tgaacccaac atactccgac cagctgaggg taacactttg ttcgcagcgt tcataatccg
     2461 acgaaatgat catagtggta tgcgggtcgg ttatcatact cttataaagc agtgtatcga
     2521 aggcagctat ccggtatgtt tcggtctgta cagaagcctg tgaatttacg ttgatatatt
     2581 gcttcgaaag cctacccctt acagtatcaa tggtaaccga tgtagagccc actacttgat
     2641 aaataatata cccagccaca tcagccgaat cgcttgggtt ccatttcagt gtaacctcac
     2701 cgggaagcac acctggggta acactggccg ataaaaaccg tggggcatcg ggaatatcaa
     2761 cttgcgaata tgcagttgtg taagaaaaca acagcatgaa acccaagaat attatgtttc
     2821 tataatgttt catcgcgtgc aaaattaaac attatacaac tataaaacga aattttgcta
     2881 taaataggtt gttttcttgc gaaaattaat tcaaggtttt tataaaaacc agctcaccat
     2941 cgagaaatgt ctgcttaaaa cccattttaa taagatagtt gctatgccgg taatgatacg
     3001 acttcgcttt cagggtgttg attcccatgc gcttgaagta gcctgtgttt ttgaagtaaa
     3061 tataatttcc gactgaataa tcgcgatatt ccggtt
//
LOCUS       DAOOQP010000203         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_40836_length_2929_cov_4.955811, whole genome shotgun sequence.
ACCESSION   DAOOQP010000203 DAOOQP010000000
VERSION     DAOOQP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40836_length_2929_cov_4.955811"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>2929
                     /locus_tag="PLK75_12775"
     CDS             129..>2929
                     /locus_tag="PLK75_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40164.1"
                     /translation="MKQLFTIFAFILCVQLLLAQQPGDLKITDEVKHITSIKSAKAAG
                     DILWEVTFDEASPVWTFGQITGTKTWNVTDTTPNWGYTNPNYQGGSVPVPPLWLYMGW
                     NYVHEYSISGANFAWVDGISDLLGAGGNTPQICNTYIQFDGIDLTGVTAPKLSFYQDY
                     KGFNGDHCYIDFSINGGSSWQTLEINQEITGNAYAAPLFELFVGSYIANQSNVSIRLR
                     WETTGTTTSGYGYGWQVDDIRIVENALNDVKLVDARMNFFEYVDYTVAGQEPYFHSSS
                     HYGMIPNEQFSSEFANMWFNGIFKNNGMNSATPSFNVKVLNPQNEEIFNETVTATNNY
                     TTSQIDTLDLIEVDFSLPTGFTSGRYTIIYNIYVTGIEDTNPIDNTDTTYFYVSDNVF
                     ARDLGNMTSKTGPCTWLDGNMDGEMIGTNYLFLYNTEVTSIDVFISEQTTPGTEIIGH
                     VMEHNEADGTWIDISTTPLFEIQQNDIGSWVTLTLTDPAIIDLAGEDGKSVKAAIEFY
                     YGSEDNEIWIGVDPTVIDSFWGVTWFLVSGSSANGWYSFTNWANGGLGIRLLTDDENY
                     DCNIDVNMGDDINQCGGSVTLDAGSGFSSYTWNGVSGSRYLAVNTSGSYSVVVTDNMG
                     CTASDIINVTINPIPVVNLGDDISQCGGSVTLNAGAGFSSYNWNGSPGNQIYSVSSSG
                     LYTVVVSNSYGCTASDQIQVNINPIPTVNLGSDITQCGGSVTLDAGAGFASYIWNGVA
                     GTRYKTVSTSGNYTVIVGNSYGCTATDAVQVTINPAASVNLGNDIEQCGGSVTLDAGA
                     GFTSYTWNGVIGTRYKTINSSGNYSVVVQNSYGCSATDAITITINTVPTVSLSMTQES
                     NNGASDGSAMAIATGGTPPYQYLWSNDGVGETITNLTNGLYCVQVSDANGCSATECIT
                     VTVSGQSNPPTAQFSADQT"
BASE COUNT          883 a          542 c          640 g          864 t
ORIGIN      
        1 atgtcaaaga acaaagagat ttatttagtg actaaaattt aacgcatcag cagtatatta
       61 aaactgataa tattttttaa ttattttgaa aacaattgat tatcaatact aaattaacaa
      121 ttaataatat gaaacaatta tttacaattt ttgcatttat tttgtgtgta cagttattgt
      181 tagcacagca accaggtgat ttaaaaatta ccgatgaagt aaaacacata acgagtataa
      241 aatctgcaaa agcggcaggt gatattctgt gggaagttac ttttgacgaa gcctcacctg
      301 tttggacttt tggtcagatt accggtacaa aaacatggaa tgttaccgat accactccca
      361 actggggcta cactaaccca aactatcagg gtggcagtgt tcctgttccc ccactatggc
      421 tttacatggg gtggaattat gttcacgaat attcgatttc gggagctaac tttgcatggg
      481 ttgatggcat ttccgattta ctcggtgccg gtggaaacac tcctcagata tgcaatactt
      541 acattcagtt cgatggtatt gacctgactg gcgttactgc tcctaaatta tctttctatc
      601 aagattacaa aggtttcaac ggagaccatt gttatattga tttttcaata aatggtggat
      661 caagctggca aaccttggaa attaaccaag aaataactgg aaatgcgtat gcagcacccc
      721 tcttcgaact ttttgtcgga agttatattg ccaatcagtc aaatgtttct attcgtctcc
      781 gctgggaaac cacgggaaca actacttccg gttacggata cggctggcag gttgatgaca
      841 taagaatcgt tgaaaatgca ctgaacgatg taaaactggt tgatgcccgg atgaatttct
      901 tcgaatatgt tgattatacg gttgccggtc aggaacctta ttttcattca agctcacatt
      961 atggaatgat tccaaacgaa cagttttcaa gtgaatttgc caacatgtgg ttcaatggta
     1021 tattcaaaaa taatggtatg aactccgcaa ctcccagttt caatgttaaa gttctgaatc
     1081 cccaaaatga agagattttt aatgaaacgg taaccgctac aaataactat accacaagtc
     1141 agattgacac acttgattta attgaagttg atttttcatt acccacaggt tttacatcgg
     1201 ggaggtatac gattatttat aatatatatg ttactggaat tgaagataca aatccaattg
     1261 ataataccga tactacctat ttctatgtct ctgataatgt ctttgcacgt gaccttggaa
     1321 acatgacatc caagacaggc ccatgtacat ggttagatgg taatatggat ggtgaaatga
     1381 ttggtaccaa ttatttgttt ctttacaaca ccgaagttac aagcattgat gttttcatta
     1441 gcgaacaaac gactccagga acagaaatta tcggacacgt tatggagcac aacgaagcag
     1501 atggaacatg gattgacata tctacaactc ccttatttga aattcagcaa aatgatattg
     1561 gctcatgggt aaccctaaca ttaacagatc cggctataat tgaccttgcc ggtgaagacg
     1621 gaaaatcggt taaagcagct atcgaattct attatggatc ggaagataat gagatatgga
     1681 tcggggttga cccaaccgtt attgacagtt tctggggtgt tacatggttc ctggtttcag
     1741 gttcaagtgc caatggatgg tattcgttca ccaattgggc aaacggtggt cttggcattc
     1801 gcctgttgac agacgatgaa aactatgatt gcaatattga tgttaatatg ggtgatgata
     1861 tcaaccaatg tggtggcagc gttacattgg atgccggctc cggattcagc agttacactt
     1921 ggaatggggt atccggtagt aggtatcttg ctgtaaatac aagcggatcg tattctgtag
     1981 tcgttaccga taatatgggt tgtacggctt ccgatattat taatgttact attaatccga
     2041 ttcctgtagt aaacctagga gatgatatca gccaatgcgg agggagtgta acgctgaatg
     2101 ccggagccgg cttcagttca tataattgga acggtagtcc tggaaatcag atttattcgg
     2161 tatcctcatc ggggttatat actgtagttg taagcaacag ctatggatgt acagccagtg
     2221 atcagattca ggtaaatatt aatccgattc caacagtaaa tttgggatca gatattactc
     2281 aatgtggtgg gagtgtaacc ttggatgctg gagctggttt tgcttcctat atttggaatg
     2341 gagtggcagg tacccgctac aagactgtca gcacatcggg gaactacacc gtaattgttg
     2401 gcaatagtta cggatgcaca gccaccgacg ccgttcaggt aactataaac ccggcagctt
     2461 cggttaattt aggcaacgac attgaacaat gcggaggtag tgttaccctt gatgcaggtg
     2521 caggatttac atcttacaca tggaatggtg taataggtac aaggtataaa acaataaata
     2581 gctcggggaa ctattctgtt gtagtgcaaa attcttatgg atgctctgca actgatgcta
     2641 ttactattac tattaacacg gttcctacag tttcgttgag catgactcaa gaatcgaata
     2701 atggagccag cgatggttcg gctatggcaa ttgccacagg agggacgccc ccgtaccaat
     2761 atttgtggag caacgacggt gtgggtgaaa ctattacaaa tttaacaaac ggtttgtatt
     2821 gcgttcaggt tagcgatgca aatggatgct ctgcaacaga gtgcattaca gttactgtat
     2881 ctggacaatc gaatccaccc acagcccagt tttcggccga ccaaaccga
//
LOCUS       DAOOQP010000204         2908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_41206_length_2908_cov_4.464423, whole genome shotgun sequence.
ACCESSION   DAOOQP010000204 DAOOQP010000000
VERSION     DAOOQP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41206_length_2908_cov_4.464423"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2009
                     /locus_tag="PLK75_12780"
     CDS             <1..2009
                     /locus_tag="PLK75_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40165.1"
                     /translation="SSFSNVAGGTPNGATYTNPNTTSLTVANISLVGTYYYRLSATST
                     GSGCTSPAVSTAATVQVVADPSVTASGATTICSGLTANLSSSGSNGTGTMSYQWQYYN
                     GSTWVNTGTNSNSYTTQSLSVTTDFRCLYSGTGPGCTTATSNTVTVTVSTPNPSGLTS
                     GDYVWTGAVNTDWNNASNWVAYNGTNFIVPATVPAISNDVLFTTYGTSCGVSTPTISS
                     ASGNCHDFRIDAGHTVVMSNSQTLNVQGSWTNDGTFSAGNGTVVFNGSSQQAILAGGS
                     AFNNVTFSNTTVGTTDINIREPMIINGVALFANGVVYYTGTGSLLFGSSATSNYGAVN
                     SYVDGIVSKAGSSSFIFPIGEGVVWAPIGIAAPSASATISADYNFTAAELNWSASYMC
                     DESQMQYTSGVEHWNLTTSGPTPAVTLYWFDGSRSGIADLSDLAVAHFNGTCWEYMSG
                     TTTGDALQGTITSSVAFSSYSPVSFGSKHRLNPLPIELVFFDATCNNGRTDLEWQTAS
                     EINNDYFTIEKSLNGTDFERVAVITGAGNSNAIINYYYQDITPHNGTTYYRLKQTDYD
                     GTFVYSYMVAVECDRVDTGNPEISICPNPFTDQIIISGTGLKSDYATISIVDVLGKEY
                     VSVPAGITDSEFSLIITLSELPPAVYFVKVKSNDYVNSIKVVKE"
     gene            2167..>2908
                     /locus_tag="PLK75_12785"
     CDS             2167..>2908
                     /locus_tag="PLK75_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40166.1"
                     /translation="MQGSKKILGTKFQKIYSIGLFLMLGVLVFLLFPKQSHFAYDFKK
                     GSPWLGENLIAPFDFAISKTPEEIKAERDSIKASFIPYYVIDNTVDDTVIGQFVNALD
                     EFFPNQNDSHNIQETDENQFFVNRLPGNFRDALTQLLVTSVNSIYRKGVIPASDSLSD
                     FPNASINLVEGNINKGLQNRSEFYTPDEALSVLKNELISKLQLQFHSGDSLIPYMVEE
                     VFSVNFFKPNIVADVRTTYELLKQKYANL"
BASE COUNT          850 a          589 c          617 g          852 t
ORIGIN      
        1 ggtcttcgtt cagcaacgtt gccggcggca cacctaacgg agctacctat accaacccaa
       61 acactacgag tttgactgta gcaaatattt cgctggttgg tacatactat taccggctat
      121 cagcaacttc cacaggaagt ggttgtactt ctccggcagt aagcacagct gcaacagttc
      181 aggtagttgc tgacccatca gtcacggcat caggagcaac taccatctgt agcggattaa
      241 cagccaattt aagctcctcc gggtctaacg gaaccggaac catgtcgtat caatggcaat
      301 attataatgg atccacatgg gttaacacgg gtacaaacag caattcttac actacccagt
      361 cactgtccgt tacaactgat ttcagatgtt tgtacagtgg cacaggcccg ggttgtacaa
      421 cggctacttc aaataccgta acagtaaccg taagtactcc taacccatca ggtttaacat
      481 cgggagatta tgtttggaca ggcgctgtca acacggattg gaataatgct tccaactggg
      541 ttgcatataa tgggacaaac tttattgtac ccgcaacagt acctgcaatt tcgaatgatg
      601 tgttgtttac aacctatgga acaagctgtg gagtttcaac tccaacaata tcttctgcgt
      661 cgggaaattg tcatgatttc aggattgatg caggacatac ggttgtgatg tcgaacagtc
      721 agactttaaa tgttcagggg agctggacta acgatggtac tttttctgcc ggcaatggaa
      781 ctgttgtttt caatggctct tctcagcaag ctattctggc aggaggaagc gcattcaata
      841 atgttacatt tagtaataca actgttggta ctaccgatat taacattagg gaacctatga
      901 tcattaatgg agtagcgcta ttcgccaatg gcgttgtgta ttatactggt actggctctc
      961 ttctgtttgg cagctcggct acatcaaatt acggagcagt gaacagctat gtggatggaa
     1021 ttgtttcgaa agctgggtcc agttctttca tttttcctat tggtgaaggt gtggtttggg
     1081 ctccaattgg tattgcagca ccatcggcat ctgctactat tagtgctgat tataatttca
     1141 cggcagctga acttaattgg tctgcatcat atatgtgcga cgaaagtcag atgcaatata
     1201 ccagcggggt tgagcactgg aatctaacaa caagcggtcc aactcctgcc gtaacccttt
     1261 attggtttga tggcagcaga agtggtattg cagacctaag cgatcttgca gtggctcact
     1321 ttaatggtac atgctgggaa tacatgagcg gaacaactac cggcgatgca ttgcagggaa
     1381 ctattacctc ctcagttgca ttttcatctt atagcccggt aagctttgga agcaaacaca
     1441 ggctgaatcc cttgcctatt gagcttgttt tctttgatgc cacctgcaac aatgggcgga
     1501 cagaccttga atggcaaact gcatcagaaa taaacaacga ctatttcaca atcgaaaaat
     1561 cgttgaacgg aaccgatttc gaaagagttg cagttataac cggggcagga aacagtaatg
     1621 ccattatcaa ctattattat caggatataa caccacataa tggtactaca tattatcgcc
     1681 tgaaacaaac cgactacgac ggaacttttg tctactccta tatggtagca gttgaatgtg
     1741 accgggttga tacgggaaat cccgaaatct caatttgccc taatcctttt accgatcaga
     1801 taattatatc cggtacaggt ttgaaatcag actatgcaac aattagcatt gttgatgttc
     1861 tgggtaaaga atacgtttcg gttccggctg gcattactga cagtgagttc tcgctgatta
     1921 ttacactttc ggagttaccg cctgcggtgt attttgtgaa agtgaaatcg aatgattatg
     1981 taaattcgat taaagtggtc aaagaataaa atttgaaaaa ttttaaaagc gcccttcggg
     2041 gcgtttttgt tttcagttgt attgtatatg taataaatga aaaggtgctc cataagcttg
     2101 gttaaactct ctataacctt agcagcttga ataaataaaa ttttctattt ttacataaaa
     2161 tttactatgc agggatcaaa gaaaatatta ggaacaaagt ttcagaaaat ctactccatt
     2221 ggattgtttc taatgctagg agtactggtt tttttgctat ttccgaagca aagccatttt
     2281 gcatatgatt tcaaaaaagg aagtccgtgg ttgggtgaga atcttatcgc ccctttcgat
     2341 tttgcaataa gtaaaacgcc agaagaaatc aaagcggaac gcgacagcat aaaagcctcc
     2401 tttattccgt actatgtcat cgataatact gttgacgaca ccgtgatcgg gcaatttgta
     2461 aatgctctcg acgagttctt tcccaatcag aatgattcgc ataacattca ggaaacagat
     2521 gagaaccagt tttttgttaa tcgtctgccg ggtaatttca gggacgcact tacccaatta
     2581 ttggtgacat ctgttaatag catatacagg aaaggtgtta ttcctgcatc agattccttg
     2641 tcagactttc ccaatgccag tattaacctt gtggaaggaa atattaataa gggacttcag
     2701 aaccgatcgg aattctacac tcctgatgaa gcattgtcag tacttaaaaa cgaacttatt
     2761 tcgaaacttc agttgcagtt tcattcaggc gattcattaa ttccatacat ggttgaagaa
     2821 gtttttagtg ttaatttttt taaacccaac atagttgctg atgtaagaac aacttatgaa
     2881 ttactgaagc aaaaatacgc gaacctcg
//
LOCUS       DAOOQP010000205         2888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_41625_length_2888_cov_4.562301, whole genome shotgun sequence.
ACCESSION   DAOOQP010000205 DAOOQP010000000
VERSION     DAOOQP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41625_length_2888_cov_4.562301"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..510
                     /locus_tag="PLK75_12790"
     CDS             256..510
                     /locus_tag="PLK75_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40167.1"
                     /translation="MLSITSPKKSGALRDTRAHATKREGPQFTFVGFPFSPDSEEPID
                     MVFFRGGKHSKVVRYKVDDYNHNGKYPSDHLPVVVWLQME"
     gene            complement(800..2479)
                     /gene="ettA"
                     /locus_tag="PLK75_12795"
     CDS             complement(800..2479)
                     /gene="ettA"
                     /locus_tag="PLK75_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOY40168.1"
                     /translation="MSDDNKIIFSMVKVSKTFPPAKQVLKDIYLSFFYGAKIGVIGLN
                     GSGKSSLLKIIAGEDTAYNGEVVFSPGYSVGYLHQEPVLDKSKTVKEVVMEGVKEIAD
                     ILHEYEEINIRFAEPLDDDEMNRLIARQGELTELIDQHDGWELDSKLERAMDALRCPE
                     ADTPVEHLSGGERRRVALCRLLLQQPDVLLLDEPTNHLDAESVEWLEMHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSARLAREEKSESKRRKTLER
                     ELEWVRMAPKARHAKAKSRLNAYEKLLNTDVKDKEEKLEIYIPNGPRLGNKVIDAKAV
                     SKAYGDKLLFENLNFSLPPNGIVGVIGPNGAGKTTLFRLIMKQATPDSGTFDIGETVA
                     LAYVDQQHSEVDPEKSVFEVISGGTEEIMLGGKMMNARAYISRFNFSGTDQSKKTGVL
                     SGGERNRLHLAMTLKSEANVLLLDEPTNDIDINTLRALEEGLDAFAGCAVVISHDRWF
                     LDRICTHILAFEGDSQVSFFEGNYSEYEENRRKRLGNDTPKRIIYRKLMKH"
     gene            complement(2603..>2888)
                     /locus_tag="PLK75_12800"
     CDS             complement(2603..>2888)
                     /locus_tag="PLK75_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40169.1"
                     /translation="RRDTVSGINSIADNVFKIAYNNDTRDLYVSAFNGSDSINIAIYD
                     ANGKQMFTKTSRLYTSSSSERISLSAFTPGIYVVKVQYEEKSYTDSIVIY"
BASE COUNT          779 a          675 c          566 g          868 t
ORIGIN      
        1 ttgtgaaatc catgtcccaa atttaaaact ttccactaaa tttgcagtag aaccgtttaa
       61 attaataata tgaaccaatt tatttttgct aaaatattaa aaaaacaatt attattcgag
      121 aaaagtaaaa ttaatttgat tatttctaat taattgataa tcaatatata atatatacat
      181 tattctattt aaatccaaca taatgcttca gaaaacatca agccggcctt agatagtctt
      241 ccatctcgcc ctcatttgct atcaattaca tccccgaaaa agtctggcgc actgcgggac
      301 acacgggctc atgcaacaaa acgcgaaggg ccgcagttta cttttgttgg atttccgttt
      361 tcccctgatt ccgaagagcc catagacatg gtgtttttca ggggaggcaa gcattcaaaa
      421 gtagtgcgat ataaagttga tgactataac cataacggga aatacccgtc tgaccactta
      481 cccgtagtgg tttggctgca aatggagtaa gtagatgata tttgatgtag ggtagtaata
      541 gccagccccg aggtattgaa ataaccgccc gtaaatacag attgcttcat gttcgttcct
      601 ctcattcgca atgacgggct ccctaacgtc attgcgagcg aagcgaagca cctgtgctga
      661 gcgaagtcga agcaatctac caacacggag acgtatcatt tataaccgcc cgaaaatgca
      721 gattgcttca tgtccgttcc tctcattcgc aaagacgggc ggcttttacg ctctattgaa
      781 ccctaccaat gacagtcggt taatgcttca taagtttgcg gtatatgata cgttttgggg
      841 tatcattacc caatcgtttg cggcggtttt cctcatattc gctatagttg ccctcgaaaa
      901 agctcacctg cgaatcgcct tcaaaagcca gaatgtgggt acagatgcgg tcgaggaacc
      961 agcggtcgtg cgaaatcacc accgcgcatc ctgcaaaagc atccaaccct tcttccaatg
     1021 ccctaagtgt gttgatgtca atatcgttgg taggctcatc aagcagcaac acattggctt
     1081 ccgacttcaa ggtcatggca aggtgcaggc ggtttcgttc ccctcccgaa agcacccctg
     1141 ttttcttgct ctggtcggtt ccactgaaat taaacctcga aatatacgcc ctggcattca
     1201 tcatttttcc gccaagcata atttcttcag ttccgcctga aataacttcg aaaacgctct
     1261 tttcggggtc gacctcgctg tgttgctggt cgacatatgc caatgcaaca gtttccccaa
     1321 tatcaaaggt cccgctgtcg ggagttgcct gcttcattat cagtctgaaa agggtagttt
     1381 tcccggctcc gttcgggcca ataactccaa caattccatt cggcggaagc gaaaaattca
     1441 ggttctcaaa caagagtttg tcaccgtatg ctttactcac tgctttggca tcgataacct
     1501 tgtttccgag acgagggcca ttgggaatat aaatttcgag tttttcttcc ttatctttca
     1561 catcggtatt gagcagtttt tcgtaggcat tcagacgtga tttggctttt gcatggcgcg
     1621 ctttaggagc catccgcacc cattcaagtt cgcgttccag tgttttgcga cgcttgcttt
     1681 ccgatttttc ttcacgtgca aggcgggccg acttttgctc gagccaagac gaataatttc
     1741 ccttccatgg gataccttcg ccacggtcga gttccagaat ccatccggca acgttatcga
     1801 gaaaatagcg gtcgtgggta acggcaatca cagtaccctt atattgctga aggtgcattt
     1861 cgagccactc caccgattct gcatcaaggt ggttggtagg ttcatcgagc agtaaaacgt
     1921 cgggttgttg caacagcagc cggcataatg ccacacgcct tcgctcacct cccgacagat
     1981 gctctacagg agtatcggct tcaggacaac gcaaagcatc catagctctt tcaagcttcg
     2041 aatcaagttc ccagccatca tgctggtcaa tcagttcggt gagctcaccc tgacgtgcaa
     2101 taagcctgtt catttcatca tcatcgagcg gttccgcaaa ccttatgttt atttcttcgt
     2161 attcatgcag tatgtcagct atctccttta caccttccat gacaacttct ttaaccgttt
     2221 ttgacttatc gagaacaggc tcctgatgaa ggtatccgac actataaccg ggagaaaata
     2281 caacctctcc gttatatgca gtatcttctc ctgcaataat tttaagaagg gatgatttcc
     2341 ccgatccatt taatccgatt acaccaattt tagcaccata aaagaatgaa aggtaaatat
     2401 ctttcagcac ctgttttgcc ggtggaaagg tcttgctgac cttaaccatc gaaaagatta
     2461 ttttattgtc gtcgctcata tctatatttt ttttacgaag ataaaaagag tttggcagaa
     2521 tgcatattct ttgtagcgca actacagttt ttccagatta agtaaattat tacttttata
     2581 taagcaaatt aacttttttc tattaataga ttactattga atctgtataa cttttttctt
     2641 cgtactggac tttcacaaca tagatacccg gagtaaatgc cgaaaggctg attctttcgc
     2701 ttgaagatga tgtatacaat cgtgaagttt ttgtgaacat ctgttttccg tttgcatcgt
     2761 aaatggcaat gttgatactg tccgatccat tgaaagcact aacatagaga tcccttgtat
     2821 cgttattgta tgcaatttta aaaacattat ccgcaatact gtttattcct gaaacagtat
     2881 ctcttcga
//
LOCUS       DAOOQP010000206         2844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_42485_length_2844_cov_5.351380, whole genome shotgun sequence.
ACCESSION   DAOOQP010000206 DAOOQP010000000
VERSION     DAOOQP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42485_length_2844_cov_5.351380"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..579)
                     /locus_tag="PLK75_12805"
     CDS             complement(151..579)
                     /locus_tag="PLK75_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40170.1"
                     /translation="MKEQVDYYFISKITINDILNTGRTIKGFAYLTKKYLFVMPERVE
                     DMGGVIGKMCFDLPKFKELLTKINEVEPGLFEISMMEIVPPEYVMPLSNFDKFEVNVG
                     FFIFGGLSFKVKGKKLQSATIGSAKIRQQVKDFISDIKFQ"
     gene            complement(722..2002)
                     /locus_tag="PLK75_12810"
     CDS             complement(722..2002)
                     /locus_tag="PLK75_12810"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOY40171.1"
                     /translation="MLSEDLLQFVWQHRYWDHRYNTLTNGADFEVIDTGAPNYDSGPD
                     FFNAKIKVGNTVWAGNVEIHTSSSQWFRHNHHLDKAYDNVVLHVVYKHDREVTTGNGM
                     LLPVFEMKIPEYILGTYAFLLKQPDWPACSAMISSAENEMVRFWLNRCAVERLESKVG
                     LMYQRFRQFNNDWEALWLSQLGEAMGCKVNKEPFGLLMQRTPLKLLQKYSHDLFSLEA
                     LLFGQAGLLDDIEHDDYSVRLKTEYDFIKKKFGLQGLPGYLWKFARMRPGNFPTLRLA
                     QFAALVHKHPAMVTEMLEMKNYNHISSFFSAEVSEYWHTHFRFGAHVKHSFRYLGSTA
                     VDLILINVVVPFLFFYGKYNDSEEHAELALDWLSQTKAENNTFIRRWSELGIKPANAL
                     ESQALLHLRENFCLRHRCPQCNLGNIFLTSGHKN"
     gene            2065..2595
                     /locus_tag="PLK75_12815"
     CDS             2065..2595
                     /locus_tag="PLK75_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40172.1"
                     /translation="MKKTVSYLIAGIIGVAFLAACIWVYTSKGRNRKALNLKIKTGAA
                     IIALTSLISHSCVTVTCYDVAPMNYISPLDSITENNHIFINPSNDSTVDFAIDQPQVN
                     TITYSFNTEAGSNITSGNAIIRSGSLSGAYCEFAVPVPENISTGTYYLKVFGKNLTEV
                     DTSVYLTSVFVDIVQE"
BASE COUNT          911 a          569 c          496 g          868 t
ORIGIN      
        1 aaaaaaatac aatgtcagca tccgcatcat taaaggatat ccggctgtga aaatgaaatg
       61 gaacttatcg cttggaactt atttcaatat tttatttaac taagcgtatc taattatcta
      121 atagtaagga aataaataag aagtattaaa ttactgaaat tttatatcac tgataaaatc
      181 tttaacctgc tgacggattt ttgcactacc aattgttgcc gattgcaact tttttccttt
      241 aactttaaag ctcaatcctc cgaaaatgaa aaagccaaca ttcacctcaa acttgtcgaa
      301 atttgacaaa ggcatcacat attcgggggg aactatttcc atcatactaa tttcgaacaa
      361 acccggttca acttcattaa tttttgtaag cagttctttg aatttgggca agtcgaagca
      421 catctttccg atgactcctc ccatatcttc caccctctcg ggcattacaa acaagtactt
      481 ttttgtaagg taagcaaagc cctttatggt tctgccagtg ttaagaatat cgttaatggt
      541 aattttggaa ataaaatagt aatcaacttg ctctttcatg gtgttgtggt tttggtttat
      601 gacataaaat taaccatatt tataaaagta tcaaaaaatt agtgataaaa atatgttagc
      661 atttacaaat atcataagtg attaatattg attgcaaatg aatcattgct aaaggcattt
      721 gttaattttt atgcccagaa gtaagaaaga tgtttcctaa gttacattgc ggacagcggt
      781 gccgtaaaca gaaattctcg cgcaggtgca acaatgcctg cgattctaat gcatttgcag
      841 gtttaatacc cagctctgac caacgacgaa taaaggtatt gttttctgcc ttggtctgcg
      901 acaaccaatc aagagccagc tcagcatgtt cctccgaatc gttatacttg ccataaaaaa
      961 acagaaacgg aacgactaca ttaataagaa taaggtcgac tgcggtgctc cccaagtaac
     1021 ggaagctatg tttgacatgt gcaccaaaac ggaaatgtgt atgccaatat tcggaaactt
     1081 cagccgaaaa gaacgacgaa atgtggttat agtttttcat ttcaagcatt tcggttacca
     1141 ttgcagggtg tttatgcacc agtgcagcaa attgggccag gcgcaaagtt gggaagttac
     1201 cgggtcgcat acgtgcaaat ttccagaggt agcccggcaa cccttgcaaa ccaaattttt
     1261 tcttaatgaa atcgtattct gttttaaggc gtacagaata atcgtcgtgc tcaatatcat
     1321 cgagtaagcc tgcttgccca aacaataaag cttcgagtga aaacaggtcg tgtgaatatt
     1381 tctgcagcag tttcagagga gttctctgca tcagcaaccc aaaaggctcc ttgttcactt
     1441 tacatcccat tgcttcgccc agctgactca accaaagcgc ttcccaatcg ttgttgaatt
     1501 gacgaaaacg ctgatacata agtccgactt tactttcaag cctttcaaca gcacaacgat
     1561 tcaaccagaa acgtaccatt tcattttctg ctgaactgat catggcactg catgccggcc
     1621 aatcaggctg ttttaaaaga aatgcatagg ttcccaaaat gtattcaggt atcttcatct
     1681 cgaaaactgg aagcaacata ccattacccg ttgttacctc cctgtcgtgt ttgtacacca
     1741 catgcaaaac aacattatcg tatgctttat caagatggtg attatgacgg aaccactgcg
     1801 atgaactggt gtgaatttcg acattacctg cccaaaccgt atttccaacc tttatcttcg
     1861 cattgaagaa atcaggtccg gaatcgtaat tcggtgcacc ggtgtcaatg acttcgaaat
     1921 cagcaccatt ggtcagcgtg ttgtaccggt gatcccagta cctatgctgc caaacaaact
     1981 gtaacaaatc ttcgctcaac atgaatatta ttataattat cagagtaaaa ttaaatattt
     2041 tgtaacttgc atcaaatatt aagcatgaaa aaaaccgttt catacctaat agccggtatt
     2101 atcggggttg ccttcttggc tgcgtgtatt tgggtttaca catcgaaagg caggaacaga
     2161 aaagccctga acctaaaaat taaaaccgga gctgccataa ttgcattgac atctttaata
     2221 agccactcgt gtgttactgt aacctgctac gatgtggcac ccatgaatta tatttcccct
     2281 ctcgatagta ttacagaaaa caatcacata ttcattaacc caagcaatga tagtaccgtt
     2341 gattttgcca ttgaccaacc acaggttaac acaataactt atagtttcaa cactgaagca
     2401 ggatcgaaca ttacttcagg caacgctata atacgcagtg gcagtctatc gggagcgtat
     2461 tgcgagtttg cagttcctgt accggaaaat ataagtacag gcacatatta cttaaaagta
     2521 ttcgggaaaa atcttactga agttgatact tcagtttacc tcacctctgt ttttgtagat
     2581 attgtgcagg aataatacat taatacacaa atttcgaact cgttaggttt tcacaatgca
     2641 ttgtatatct gttaattgaa aaattggtcc gtttttggta caatagttgc aacaagttta
     2701 ttatattttt gttttagaag ttttttgaaa atctgaactt ttattttaaa aatttgttta
     2761 ttaatcataa atatcattaa atttgtatta aacctaggtt tcacttatta aaactaaact
     2821 atgaaaaaaa tcatcctttc aatt
//
LOCUS       DAOOQP010000207         2801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_43417_length_2801_cov_7.865623, whole genome shotgun sequence.
ACCESSION   DAOOQP010000207 DAOOQP010000000
VERSION     DAOOQP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43417_length_2801_cov_7.865623"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLK75_12820"
     CDS             <1..599
                     /locus_tag="PLK75_12820"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HOY40173.1"
                     /translation="QLLQHGQSIEHLNLRKIGYLKSYNVFNVACVEGLPEEFYHMDEL
                     EQLSEIERDERAESMIREIGAEIVFDAQNEAYYKPSEDKIYMPLPKQFVSKEAFLSVM
                     YHEIGHWTGAAHRLNRPISNAFANEFYAFEELIAELSSAFMLAYLGYESRITDNAAYI
                     ENWLSVMKNDTKFVIQAASQAQAASDYILQLANVEIAA"
     gene            856..1296
                     /locus_tag="PLK75_12825"
     CDS             856..1296
                     /locus_tag="PLK75_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40174.1"
                     /translation="MKTKILAFVCLSLFSLVATAQSALQVKDILVTPSIEIDSISGLP
                     VDNDVENFDVFFKVNDLSAISNVHILVGTSPNSGDILLIEANLLETDGNYHLVYNGFS
                     EMLIDNIIKVKISLNNLQYTSYSYISAYVSNASQQTSNVLIFTK"
     gene            1308..>2801
                     /locus_tag="PLK75_12830"
     CDS             1308..>2801
                     /locus_tag="PLK75_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOY40175.1"
                     /translation="MKKLFIISAILLIALSGFTQNIFVVTKNTDPNPFSDPYNNIDSL
                     CDPDMYGTLQWAVRKANDAVSPSRIEFNIPGTGIHEIELQSFLPQIIKPVELDATSQP
                     GYQPGNCAIKINGQGKFKSCFEIYNATVSISGFEITGFVTNAIWLNSSPNSLILNNKV
                     ILSSNSSIFSYAIGFAMSDNVTVKGNIISNVQTSDSINSYYGMYIKYSNYGVIGDTTT
                     DGANHISNCVTGILLAQSQNVKISGNSIFNNNTAILLMNGSNNNISPPVILSYANGIL
                     SGTAGPNNTIEIFGSTGPENANEYLASTVADGNGEWYVGVLTFHAYFVSAAISVDNNA
                     SELSEIMYNTMNYNCPDVLTENCNLICNSEMLCLENNSCGENTPPTGWFSSHGSCDYC
                     QNNNSLICGLGNSQTDDMIDYVAFFSLYNTPAYSSARSEGIYSFLNMGFISGVQYTLR
                     FTKCNNYNDDYSGRVVFYVKLISAEAIDYFQLFDYNVPNVPSQYSRTL"
BASE COUNT          842 a          564 c          558 g          837 t
ORIGIN      
        1 aacaactgct gcagcacgga cagagcatcg aacacctgaa cctgcgaaaa atcgggtatc
       61 tgaaaagcta caatgtgttt aatgtggctt gtgtggaagg tctgccggag gagttttacc
      121 atatggatga actggagcaa ctcagcgaga ttgaacgtga tgaacgagcc gaaagcatga
      181 ttcgggagat tggagccgag attgtgtttg atgcccaaaa cgaagcgtac tacaaaccct
      241 ccgaagataa aatctatatg cccctgccga aacagtttgt aagcaaggaa gcgtttttgt
      301 ctgtaatgta ccatgagatt ggacattgga caggcgcagc acacaggctg aaccgcccta
      361 tttccaatgc ttttgcgaat gaattttatg catttgaaga attgattgcc gagttaagct
      421 ctgcttttat gctggcatat ttaggctacg agagccgcat caccgacaac gcagcctata
      481 tcgaaaactg gctcagcgtg atgaaaaacg acaccaagtt tgtcattcag gcagcctcac
      541 aggcacaggc agcatccgac tacattttgc agcttgccaa tgtagagatc gcagcctgac
      601 acgaaaatca acacggttcc ggtcctcatg atcaggaacc ggtttcgcta cacccagacc
      661 tccgcagcca ctgcacccga cctgtggctt tttcgtgggg aaatatcaat aaaatttgtc
      721 aagccatctg taaatctttg gcaatgatgg tagtaattga caatttctaa tttttgttga
      781 taatttgcta gcacactatt tggtcttttt taattgattt tctactttta gccatatcct
      841 taatacatca cagccatgaa gacaaaaatc cttgcctttg tttgccttag cttattttcg
      901 ctagtggcaa cagcccaatc tgcattgcag gtaaaagaca ttttggtgac tccttcgatt
      961 gaaattgatt cgatttcggg tttacctgtc gataatgatg tcgaaaactt cgacgtattt
     1021 ttcaaagtga acgacctttc agcaatttcg aatgtacaca ttctggttgg gacttctcca
     1081 aactcaggtg atattttgtt aatcgaagca aatttattgg agactgatgg gaattatcat
     1141 cttgtgtata atggtttctc agaaatgctt atcgacaata tcataaaagt gaaaatatca
     1201 ctgaataact tgcaatatac gtcttatagc tatatttcgg catacgtatc aaatgccagc
     1261 caacaaacaa gcaatgtgtt aatattcacc aaataatcgc tatcaccatg aaaaaactat
     1321 tcatcatcag tgctatatta ttgatagcct taagcggttt tacccaaaat atttttgtgg
     1381 ttacaaaaaa cactgacccg aatccatttt cggatccata caataacatc gacagcctgt
     1441 gcgaccccga tatgtacggc acgctgcaat gggctgtacg aaaagcgaac gatgcggttt
     1501 cgccatcgcg catcgagttt aatattccgg gaacgggtat tcacgaaata gagttgcaat
     1561 cgtttttgcc ccaaataata aaaccggttg agttggacgc tacctcgcag cccggctacc
     1621 aaccaggcaa ctgcgcaatt aaaattaacg ggcagggtaa gttcaaatct tgtttcgaaa
     1681 tttacaatgc aactgtaagt atttcagggt ttgaaatcac gggatttgta accaatgcca
     1741 tttggctgaa tagctcaccc aattctttaa tactcaacaa taaagtaata ctaagcagca
     1801 atagcagtat ttttagctat gccattggct ttgccatgtc tgataatgtt actgtaaaag
     1861 gcaatataat ttcaaacgtt caaacatccg attcaattaa ttcttattat gggatgtaca
     1921 ttaaatattc caactacgga gttattggcg acaccacaac tgacggagca aaccatattt
     1981 cgaattgtgt aactggaata ttattagccc aatcgcaaaa tgtaaaaata tcaggtaata
     2041 gcatctttaa taacaatacc gcaattctgc tgatgaacgg atcgaataat aacatttcac
     2101 ctcctgtgat tctgtcgtat gccaatggta ttctttcggg aaccgctgga cccaacaata
     2161 ccatagaaat ttttgggagc acaggccccg aaaatgccaa tgagtatttg gcaagtactg
     2221 ttgctgatgg gaatggagag tggtatgtcg gtgtgttaac ttttcatgcc tattttgttt
     2281 ctgcggcaat aagcgttgac aacaatgctt cggaattgag cgaaattatg tacaatacga
     2341 tgaattacaa ttgtcctgat gttttaactg aaaactgcaa tcttatttgt aattctgaga
     2401 tgttatgcct tgaaaataat tcatgcggtg aaaatactcc gcctaccggt tggtttagca
     2461 gccatggttc atgtgactat tgtcaaaata acaatagcct aatttgtggg ttgggcaata
     2521 gtcaaacaga tgatatgatt gattatgtag cttttttttc cttatataat acccctgcgt
     2581 attcctctgc acgctcagaa ggaatttatt ctttcttaaa tatgggattt atttccggcg
     2641 tgcagtacac cttacgtttt acgaaatgta ataattataa tgacgattat tccgggagag
     2701 ttgtttttta tgtgaaacta atcagtgctg aagcaataga ctattttcaa ctttttgatt
     2761 ataatgtacc caatgttccg agccaatatt cccgaacttt g
//
LOCUS       DAOOQP010000208         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_46169_length_2677_cov_4.161709, whole genome shotgun sequence.
ACCESSION   DAOOQP010000208 DAOOQP010000000
VERSION     DAOOQP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46169_length_2677_cov_4.161709"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2677
                     /locus_tag="PLK75_12835"
     CDS             <1..>2677
                     /locus_tag="PLK75_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40176.1"
                     /translation="FASVGPWGTIYGANAADPGWSVQIYDCEGVDVGSLTRTTITFIG
                     QTDCGQATFVYDSGSINSAINDNSCSASTASLYIVPPGAPAGSYTVSSQIVNQEWTCS
                     AIPGWSSNTLSTQIVAGTEDFPPSTCIYSLTVYEQINVPGNIQCQTSAQQTFETLPAD
                     ATIEPVSPMCANAAPYQLEAEDGGGTWSCTSVPAAVENGTFYPDIAGPGNHTITYAIV
                     GPCSDNDQITITVYDNITVSNFTDECNDINTEYTISFDVTGSQGGPVSFLANFGSGFQ
                     SFTGSFTHNFPSGTIYNITVTDANQCSEFVYSGYHYCDCETYAGTMVSLEPILLCANE
                     CTDTDLHNGNHVLDGNDMVEFVLHNGAYPPTIYSQSNNTSFCMNDVPGGAQFGTTYYV
                     SAVAGDNVGGHVNAADICYNICIGTPVRWFPVPIAHINGTELDTCALSIHLSASPPDE
                     GMIAYWTANADFVPLGNSMISSPEIDVLVNTYADVTFTWHVTNGVCSGTDEIMVHFLQ
                     TPNAFAGNDTIVCGTCTDLSAVLSLPSSNGQWSGNGASFAASTNPGSEVCVTTYGTYV
                     FTWTEYNGDCFDQDNVTVTFVQEPTPTTSMNHDTVCGVTYNLVVYNSQNPGQWRAYDD
                     GVQIFPTYVNGTSQSTSTTVIIPPYDGLYNTIDFEWTEISNYGGLQCFGTASVSVTFA
                     REPSASVGPDNWEQVCGNCFTFAADTIGSGWAFGTWVAKDIICEFDDINDPHATVCID
                     SLGSFGDTAHVAVSFLWIMNNTGCTSIDTMYVTFYNEPIANAGVNDSICGYEYELHAY
                     YSIPLSGGYTPTGWWYFHDGPGVPSILNQSSPVTSVTVSAPGTYRFVWRETNTNMPSC
                     YTTDTVTIKFVEIPLIFAGDDFNVCGQCTTLNCTSA"
BASE COUNT          733 a          612 c          575 g          757 t
ORIGIN      
        1 tttgcctctg taggaccctg gggaacaatt tatggcgcca acgccgcgga cccgggctgg
       61 tcggtacaga tatacgactg tgagggtgtg gatgttggct cgcttacccg aacaaccatc
      121 acctttattg gtcagaccga ttgtggacag gcaacatttg tgtatgattc cggatcaata
      181 aactctgcca taaacgacaa ttcatgcagt gcatcaaccg cttctctata tatcgttcct
      241 ccgggtgcac ccgcaggatc atatactgta tcttctcaaa ttgtgaatca ggaatggaca
      301 tgcagtgcta tacccggatg gtctagtaac acactgtcaa cccaaattgt agcaggtacc
      361 gaagattttc cgccatccac ttgcatttat tcattaacag tttatgaaca gataaatgta
      421 cctggaaaca ttcaatgtca gacctcagca caacaaacat ttgaaacctt gccggctgac
      481 gctactattg aacccgtaag cccaatgtgc gccaatgcag caccatacca actggaagct
      541 gaagacggtg gcggaacatg gagttgcaca agcgttcctg ctgctgttga aaacggcact
      601 ttttatcccg acattgctgg acctggcaat catactataa cctatgcaat tgttggtccg
      661 tgtagcgata acgaccaaat tacaataacc gtttacgaca atatcactgt gagtaatttt
      721 actgatgaat gcaatgatat taatactgaa tacactatca gctttgatgt aacgggctca
      781 cagggtggac ctgtttcatt cctagcaaat ttcggatcag gatttcaatc attcacaggt
      841 tcttttaccc acaattttcc ttcaggcact atatataata ttacagtaac tgatgccaac
      901 caatgttcag agtttgttta ttccggatat cactactgcg attgtgagac ctatgccgga
      961 actatggtca gtctagagcc catattactc tgcgctaatg aatgcactga cactgacctg
     1021 cacaacggga atcatgttct cgacgggaac gatatggttg agtttgttct gcataatggt
     1081 gcatatcctc caacaatata ttctcaaagc aacaacacaa gcttttgtat gaacgacgtg
     1141 ccaggtggtg cccaatttgg aactacatat tatgtttcag cagttgctgg agataatgta
     1201 ggaggccatg tgaatgctgc tgatatttgt tataatattt gcatcggaac accagtacgt
     1261 tggtttccag ttccaatagc tcatattaac ggtactgaac tagacacatg tgccctttct
     1321 attcatttga gtgccagccc accggatgaa ggaatgattg catactggac agctaatgct
     1381 gattttgttc cactggggaa ctccatgatc tcaagccctg aaattgacgt tctggtaaat
     1441 acttatgctg atgttacctt cacatggcat gtaacgaatg gagtttgttc gggaacagat
     1501 gaaattatgg tacatttcct ccagacacca aacgcttttg caggcaacga tacaattgta
     1561 tgcgggacct gtaccgatct gagtgccgtg ttaagtttac ccagctcaaa cggtcaatgg
     1621 agcggtaatg gtgcttcgtt tgcagcttca acaaaccctg gatctgaagt gtgtgtaacc
     1681 acttacggaa cttatgtatt tacatggaca gaatacaatg gcgattgctt cgatcaggac
     1741 aatgttaccg ttacctttgt tcaggaaccc acgccaacaa catcaatgaa tcatgatact
     1801 gtttgcggtg ttacctacaa ccttgtcgtt tacaactccc aaaaccccgg gcaatggcgc
     1861 gcatacgacg acggtgtcca gattttcccg acttatgtaa acggtacaag tcaaagcaca
     1921 agtactactg taattatccc tccctacgat ggattatata ataccattga tttcgaatgg
     1981 actgagataa gcaattatgg tggattacag tgtttcggca cggcaagtgt atcagtaacc
     2041 tttgcccgag aaccttccgc aagtgttggt cccgataact gggagcaggt ttgtggcaac
     2101 tgcttcactt ttgcagctga cactattggc tctggctggg ctttcggtac atgggtagca
     2161 aaagatatca tctgtgaatt cgacgatatt aacgaccccc atgccacagt ttgtatcgat
     2221 tctttaggaa gtttcggcga tactgcccat gttgcagttt catttttatg gattatgaat
     2281 aacaccggat gtacgtctat cgatactatg tatgtgacat tttataatga gcccattgcc
     2341 aacgccggtg taaacgattc catctgtggt tacgaatacg aattgcatgc atattacagc
     2401 ataccactat ctggtggata tactcccaca ggctggtggt atttccacga cgggccgggt
     2461 gttccatcaa ttctgaatca aagcagccct gttaccagtg ttacggtttc agctccgggt
     2521 acataccgct ttgtatggag agaaacaaat accaatatgc caagctgcta cactaccgac
     2581 acggttacga ttaaatttgt tgaaatccca ctaatttttg ccggtgacga ttttaacgta
     2641 tgcggtcaat gcacaaccct gaactgtacc agtgcag
//
LOCUS       DAOOQP010000209         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_46841_length_2650_cov_3.440462, whole genome shotgun sequence.
ACCESSION   DAOOQP010000209 DAOOQP010000000
VERSION     DAOOQP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46841_length_2650_cov_3.440462"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2179
                     /locus_tag="PLK75_12840"
     CDS             <1..2179
                     /locus_tag="PLK75_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40177.1"
                     /translation="ESNEDTEIKTYYIIVGSSGSTAESAWILQHTHFTSPYRGVFQVD
                     IPDENTAWVLTYDGTGGTYTTDFARTADGGSTWIPGVLDIPSELKPGDISAIDDQHAW
                     LAAYNTAGGGGIYYTADGGETWTHQSTASFSGSSSFCNVVHMFNENEGYAMGDPESNE
                     FEIYTTANGGETWTRVNGANIPNPETDEMGWTGVQDAYGDIAWFGSSTGRIFRTSDKG
                     QTWQAFNTGEDNVSRISFWDDQNGIIICQVTNQSTGAIESWNMRKTSNGGETWSLINI
                     SGGYQFSDVSVVPGNLGMVVSVNISQTVGDNFSAISMDYGTTWEMLDDSIQYTCVEFL
                     NEENGWAGSFNLDAENGGIYKWAGNPGENPYFVSSPITEVDEMAVYTYNIEVIDPANG
                     TITINAASGDPLPEWLTLTDNGDGTAVLTGTAPEVSAPVENFPVVIEASNGTVDISQE
                     FTINVNTIDLAPIFTSEPITSNFVNVLYTYNITTSDPDGDDLVITAVQKPSWSTFVDN
                     GDGTAVLTGTPTTTSMLGFQVKLQVTDGIYTAEQNFSIQVTQNHAPEFTSEPVVSGTT
                     NTVYLYNITATDDDDQPITITATTLPEWASLTDNGDGTAIMTGTPLNPSTEGYPVVLQ
                     VSDGMLSTDQEFSIVVVNSSIIDFGMGMISLYPNPTVDVLYITNAMNSEYQLFDILGH
                     CLYSGVINTASFAIDVEGLSSGNYIVKLKNDGQYGVLRFEIVK"
     gene            2230..>2650
                     /locus_tag="PLK75_12845"
     CDS             2230..>2650
                     /locus_tag="PLK75_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40178.1"
                     /translation="MKVNVVIELVGLCLTGAGLFFKVRLQKYFIVFAGLLLITGGFVY
                     EANWIAVALCLLFGAFVTLNFVKTRRSFKEVRVVEIPLDDIYLNEFLRYYSKSIYNIF
                     PFFELFETHKAFLLVREMNVAGVLLTVQEGDVLNIDVD"
BASE COUNT          751 a          519 c          615 g          765 t
ORIGIN      
        1 cgaatcaaat gaagacactg agattaaaac ttactatatt attgtaggta gttcaggaag
       61 caccgccgaa agtgcatgga ttttacagca tactcatttt acatcccctt acagaggcgt
      121 atttcaggtt gatattcccg atgaaaatac ggcatgggta ctcacctacg atggaacagg
      181 aggtacttat acaacagatt ttgcacggac cgctgacggt ggtagcacct ggattcccgg
      241 agtgcttgat attcccagcg aattaaagcc aggagatatc tctgctatcg atgatcagca
      301 tgcatggctt gctgcctata acacagccgg tggcggtggt atttactaca ctgctgacgg
      361 aggtgaaaca tggactcatc agtcaacagc tagcttttct gggtcatcca gtttttgtaa
      421 cgtagtacat atgttcaacg aaaacgaagg ctatgctatg ggagaccctg aaagcaatga
      481 attcgaaatt tatactacgg caaacggagg tgaaacatgg acacgtgtaa atggtgccaa
      541 tattccgaac cctgaaacgg atgaaatggg ttggacaggg gtacaggatg cctacggcga
      601 tattgcatgg tttggctcat ccacaggtag aatattccgc acatccgata aaggccaaac
      661 atggcaggct tttaatacag gtgaagacaa tgttagccgg attagtttct gggacgatca
      721 aaatggtatt ataatttgtc aggtaaccaa tcaaagcaca ggagcgattg aaagttggaa
      781 tatgcgcaaa acttcgaatg ggggtgaaac atggtcactt ataaacatca gtggtggtta
      841 tcagttctct gatgtttcag tggttccggg aaatttaggg atggtagtaa gtgttaatat
      901 atcacaaacc gttggcgata atttctcagc aatcagtatg gattacggta ctacatggga
      961 aatgttggac gacagtattc aatatacctg tgtcgagttc cttaacgaag aaaacggctg
     1021 ggcaggttct tttaaccttg acgcagaaaa tggcggtatt tacaaatggg caggaaaccc
     1081 cggtgagaac ccttattttg tttcctctcc gataaccgag gttgacgaga tggcagttta
     1141 cacttacaac atcgaagtaa ttgaccctgc aaacggaact attaccataa acgcagcctc
     1201 cggtgatccg ctgcctgagt ggctcacact taccgacaac ggcgatggca ctgctgtgct
     1261 tactggaacc gctcccgaag tgagtgcccc cgtagaaaat ttcccagtgg tgattgaagc
     1321 cagcaatgga actgttgata ttagtcagga gtttacaatt aatgtgaata caattgacct
     1381 ggctccaata tttacatcag aacccattac aagcaatttt gttaacgtcc tttatacata
     1441 taatattact accagcgacc ccgatggtga tgatctggtg ataactgcgg ttcagaaacc
     1501 gtcgtggagt acgtttgttg ataatggtga tggaacagca gtgcttaccg gaacacctac
     1561 aaccacaagt atgttgggat ttcaggtaaa gcttcaggtt acggacggga tttatactgc
     1621 tgaacaaaat ttcagtattc aggttacgca gaatcatgcc cctgagttca cttcagaacc
     1681 tgtggtcagc ggtaccacga acaccgtgta tttatataac attactgcca ccgacgacga
     1741 cgatcaaccc attactatta cggcaacaac tttacccgaa tgggctagtc ttaccgataa
     1801 cggcgatggt acagcaataa tgacaggaac ccccctgaat cctagtaccg aaggatatcc
     1861 tgttgttctt caggtttccg acggaatgct ttctaccgat caggaattca gtattgtggt
     1921 tgtgaacagt tcgattatcg attttggaat gggaatgata agcctttacc ctaacccgac
     1981 ggttgatgtg ctttatatta ccaatgcaat gaactcggag tatcagttgt tcgatatact
     2041 tggacattgt ttgtattccg gtgtaatcaa taccgcttcg tttgcgattg acgttgaagg
     2101 cctttcatcc ggaaattata tcgttaaact taagaatgat ggacagtacg gagtgttacg
     2161 cttcgaaatt gtaaaatagt aactaacaat aatagcctgt acctttcggg tgcaggctat
     2221 ttgtttttta tgaaagtaaa tgttgtaata gaattggtgg gtttgtgcct gacaggcgcc
     2281 ggtttgtttt ttaaggttag gttacaaaag tatttcattg tatttgccgg cctgctttta
     2341 attaccggag gtttcgttta cgaagcgaat tggatcgctg tagcactttg tttattattt
     2401 ggggcatttg ttacactaaa ctttgtgaaa acgcggcgaa gttttaaaga ggttcgtgtt
     2461 gttgagattc ctctcgacga tatctatctg aatgaatttc tcaggtatta ttccaaaagc
     2521 atatataata tttttccttt ctttgagctc ttcgaaaccc ataaagcttt cttgctggta
     2581 cgtgaaatga atgttgccgg agtacttctc actgttcagg aaggagacgt attgaatatt
     2641 gatgttgatt
//
LOCUS       DAOOQP010000210         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_46880_length_2648_cov_6.729657, whole genome shotgun sequence.
ACCESSION   DAOOQP010000210 DAOOQP010000000
VERSION     DAOOQP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46880_length_2648_cov_6.729657"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..878
                     /locus_tag="PLK75_12850"
     CDS             54..878
                     /locus_tag="PLK75_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOY40179.1"
                     /translation="MKDIKKHVREAYSEIARDQKDKGESSCCAANSCCGSKYSIFSED
                     YSHLEGYNQDADLGLGCGIPTVFAGIKTGDTVLDLGSGAGNDCFIARKLTGETGEVIG
                     IDFTPEMVELAVNNLRKSGYTNVYFRLGDIEHLPVTANKADVVISNCVLNLIPDKSKA
                     FSEIFRVLKTGGHFSVSDIVLKGNVSNAVRSAVALYAGCISGALQYDDYLSKIFDAGF
                     SNIRVHSAKQVIVPDALMLQHISDEELMEFRNSGTEIMSITVSADKPKTCCCGCDC"
     gene            898..1965
                     /gene="arsB"
                     /locus_tag="PLK75_12855"
     CDS             898..1965
                     /gene="arsB"
                     /locus_tag="PLK75_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOY40180.1"
                     /translation="MPSAPRLKFLDRYLTLWIFLAMAIGVLSGYFFPVIADFWESLKS
                     SPESTTNIPIAIGLILMMYPPLAKVKYEELGDVFKNYKILTLSLVQNWIIGPVLMFAL
                     AIVLFKLFPGENNANLPYMYGIIMIGLARCIAMVIVWNDLAKGNTQYAAGLVAFNSIF
                     QVLFFSFYAYLFIAVMPGWFGIPISSAVEKITIGQIAESVFIYLGIPFIAGFLTRFIL
                     IRVKNKIWYHTKFIPKISPVTLIALLFTIVVMFSLKGEYIIKLPLDVVWVAIPLLIYF
                     VVMFLGSFAMSKLIGANYEQTVTLSFTAASNNFELAIAVAIAIFGMNSGEAFAAVIGP
                     LVEVPVMIALVNVALRFRRRF"
     gene            complement(2354..>2648)
                     /locus_tag="PLK75_12860"
     CDS             complement(2354..>2648)
                     /locus_tag="PLK75_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOY40181.1"
                     /translation="VSVPIYFATKNRKKAFWLSFLSGLAEPVGAIGGYFILRSFFSGS
                     MFGLIFAGVAGIMVYISLDELLPTAEEYGEHHIAIGGLIAGMAVMATSLLFFV"
BASE COUNT          759 a          542 c          573 g          774 t
ORIGIN      
        1 tattaatatc aatgagttat gactaaaaat aaaaaattaa cgcaccacta ctaatgaaag
       61 atataaaaaa acacgttcgg gaggcatatt cagaaattgc acgcgaccaa aaagataagg
      121 gagaatcatc ttgttgcgct gcaaattcat gctgtggcag taaatattct attttcagtg
      181 aggattacag tcatctggaa ggatataatc aggatgcaga tttgggattg ggttgcggaa
      241 tacccaccgt atttgcagga attaaaaccg gtgatacagt tttagacctt ggatcaggag
      301 caggcaacga ctgttttatt gcacggaaat taacaggcga aacgggtgaa gtgattggta
      361 ttgatttcac acccgaaatg gtggaactcg ctgtaaataa tttaagaaaa tcagggtata
      421 cgaacgtgta tttccgatta ggcgacattg agcatttgcc ggttacagcc aataaagcag
      481 atgtggtaat aagcaactgt gtattaaacc ttatcccaga taaaagtaag gcattcagcg
      541 aaatattcag ggtgttgaaa acaggaggcc acttttcggt aagcgacatt gtgctgaaag
      601 gaaatgttag taatgctgtg cgatcggctg ttgcattgta cgcgggttgc atttccggag
      661 ctcttcagta cgacgattat ctcagtaaaa tattcgatgc aggattcagc aatatccgtg
      721 tacatagtgc aaaacaagtt attgtacctg atgcattgat gctacaacat atcagtgacg
      781 aagaacttat ggaattccga aattcaggaa ccgaaatcat gagcattact gtttctgctg
      841 acaagccgaa aacatgttgc tgtggctgcg attgttaaat actaaaaata taatgccatg
      901 ccttctgccc ctcgtttaaa atttctggat cgttacctga ccctgtggat tttccttgcc
      961 atggcaatcg gtgtattgtc aggatatttt ttccctgtaa ttgctgattt ctgggagtcg
     1021 ttgaaatcgt cgcccgaaag cactaccaat atccccattg ccattgggtt aattcttatg
     1081 atgtaccctc cattagccaa agtaaagtac gaagagcttg gcgatgtgtt caaaaactac
     1141 aagattctaa cactttcgct ggtgcaaaac tggattattg gacctgtact gatgtttgcg
     1201 cttgccattg tgctatttaa gctgttcccg ggcgaaaaca acgccaattt accctatatg
     1261 tacggaatta tcatgattgg tctggcccgc tgcattgcca tggttatcgt ttggaacgac
     1321 cttgccaaag ggaacacaca gtatgctgcc ggattggtgg cttttaattc tatttttcag
     1381 gtgttgtttt tctcgttcta tgcttacttg ttcatcgcgg taatgccggg ttggtttggc
     1441 atcccaatca gcagtgctgt cgagaaaatt accatcgggc agattgccga aagtgttttt
     1501 atttacctcg gaatcccgtt tatagcaggt ttcctcacgc gctttatact tatacgggta
     1561 aaaaataaaa tctggtatca cacaaaattt atcccgaaaa tcagtccggt tacgcttata
     1621 gcattacttt tcactattgt tgtaatgttc tctttgaaag gtgaatacat tataaaacta
     1681 ccgctcgatg tagtgtgggt tgccattcct ttactgatat attttgtggt gatgtttctg
     1741 ggctcttttg ccatgagtaa actcatcggt gcaaattatg agcaaacagt taccctatcg
     1801 tttactgcgg ccagcaacaa ttttgaactt gctattgctg ttgccattgc catattcggc
     1861 atgaactcag gagaagcctt tgcggctgtt atcgggcccc ttgttgaggt tcctgtaatg
     1921 attgctctgg tgaatgtggc cttgcgattt aggaggaggt tttaacattc gttgaatagg
     1981 gacacgtcaa tcgcgctgca aatactttgt gtaaacggac ctcgttgcaa acgaagtcga
     2041 gatgagaata attccccctt caaatgggaa attatttaat tatgaacacg gctttccctc
     2101 ctgctccgag ttaccggaac gggactcgga aacgctgtac tcgacgggct cagcaaagga
     2161 acttcggcgt cacggctcca tctcgacttc atctcggctt cgccatgacc agctcgatga
     2221 ccagttcgac gaccgaagat tgcttggctg tgctcgcgag caaaacctaa taaaaaagaa
     2281 ccgtatttaa ttgcttgttt ctagttgaat aaaaatcgaa cttgtgtata aaaaaattaa
     2341 tggagcataa atattacaca aaaaacaaca agctcgttgc cattacggcc attcctgcaa
     2401 tcaaaccgcc tatggcaata tgatgttctc catattcttc cgctgttggt aacagttcgt
     2461 ccagcgaaat atacaccatt attcccgcaa ctcccgcgaa aataaggccg aacattgatc
     2521 cggaaaagaa acttcgaagt ataaaatatc ccccaatagc tccaacaggc tcggctagtc
     2581 ctgataaaaa cgacagccaa aatgcttttt ttcggttttt tgtagcaaag taaataggca
     2641 ccgagact
//
LOCUS       DAOOQP010000211         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_47018_length_2642_cov_5.673367, whole genome shotgun sequence.
ACCESSION   DAOOQP010000211 DAOOQP010000000
VERSION     DAOOQP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47018_length_2642_cov_5.673367"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2642)
                     /locus_tag="PLK75_12865"
     CDS             complement(<1..>2642)
                     /locus_tag="PLK75_12865"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOY40182.1"
                     /translation="TTICAGDEIVWETGLGSFYHEWNTGYNGGSALTIGGPYPEGNYR
                     VTVYDLLGCWATSDIINVQVDNFPNQISLGADRDECYGTNIGLISPPTGIVSYLWNDG
                     TTSSVIPATESGNYHVTVTNNRGCLGIDTVQISISGYAPVVDFDADTACLGEETHFTN
                     LSTITPPFEISEWSWDFGDSETSSEENPVHTYDAPGGYMVQLYASTDAGCNNFIQKEI
                     LVLPNPEAAFSVNLGLHHCLNSDVSFVNEANDPEITSWHWDFGDGSESNEENPQHVFT
                     TSGTFEVWQKVTNSNGCSDSTMLSLNVDTDFQLPEDFHCLTPASEAYFYNLNPLVIFS
                     WTPSSNTLYYRIEISDDAGFENIIWSSDITDNSYSQAIADTGTYYWRVFAFNMCNDFN
                     ETDVDSFFVKRFSPADISFWIDASEKVSVSDNKVTAWQDKSQNDYMGLQANAILQPEY
                     NVNDSLINNQASVQFENNYINFGSILPLDSIRDYYMFIKYDQFSNYGFVLSKGVNGEG
                     AFAVNNDADLCMMRVYYDNVITTTEWCMPEDSFFLVNVRMTPMEKIEVYHNLFKVSEF
                     PISVDLIGTNLFDLIIGNSQYGDYTFPMQGEIAEIIVFDTLLNYSGRMELMNYFRNKY
                     APPVNLGRDVIVTYNFCDTTISAEKPWFVDYSWSNGETSPSIILNSMQSGVYEVTVTD
                     IFGMTSSDEIEVNFPIVNQGLQDTTICSGNSIQWQALENDGYTYQWYGNASVESYIDI
                     NQAGNYAYIVTDSLGCRFYSDTVSIAISYFATEASLGPNDTSLCIGNRIMLSTEIEEA
                     ASYHWSNGSSEPFITIEAAGEYSVTVTSIYGCVAEDTINVSIHGTAPHADFEVAGLCE
                     KNLIVFSDMSEAMSGSLTQWEWSI"
BASE COUNT          761 a          588 c          523 g          770 t
ORIGIN      
        1 ttaatactcc attcccattg agtaagagaa cccgacatcg cctcactcat gtcgctaaaa
       61 acaatcagat tcttctcaca aagtccggca acctcaaaat cagcatgagg agccgttcca
      121 tgaatgctaa cattaattgt atcctcggca acgcatccgt aaatacttgt tacggtaaca
      181 ctatattcac cggcagcttc aatggtgata aatggttcac tgctcccgtt gctccaatga
      241 taacttgcag cttcttctat ttcggttgac agcatgatgc gatttccaat acataaactt
      301 gtatcattgg gaccaagcga ggcttctgta gcaaaatagc tgattgcaat cgagacagtg
      361 tcggagtaaa acctacagcc aaggctatcg gtcacgatgt acgcataatt accggcctgg
      421 ttgatatcaa tataactttc aaccgatgca ttcccgtacc actgataggt ataaccatcg
      481 ttttccagtg cctgccactg aatcgaattt ccgctgcaaa tggttgtatc ctgaagccct
      541 tgattaacaa tgggaaaatt cacttcgatt tcatcggaag aggtcatacc aaaaatatca
      601 gttacggtaa cctcatatac acccgattgc atagaattaa gaataattga tggagaggtt
      661 tcaccattac tccagctata atcgacaaac cagggtttct ctgcagaaat ggttgtatcg
      721 cagaaattat atgtaactat cacatcgcgt cccaggttaa cgggaggagc atatttatta
      781 cgaaagtaat tcatgagttc cattctcccg gagtaattca gcaaagtgtc gaaaacaata
      841 atttctgcaa tttcgccttg cataggaaat gtgtagtcgc catactggga attcccgatg
      901 attaggtcaa acaaattcgt tccgattaaa tcgacactaa tggggaattc gctgactttg
      961 aacaaattgt ggtatacttc aattttttcc atcggtgtca tccttacatt tacaaggaaa
     1021 aaactgtctt cgggcatgca ccactccgtg gttgtgataa cattatcgta atataccctc
     1081 atcatgcata aatcagcatc gttgttcacc gcaaaagctc cctccccgtt tacacccttc
     1141 gataatacaa atccatagtt gctgaactga tcgtacttga taaacatata ataatcgcgt
     1201 atgctatcga ggggcagaat gctaccgaaa ttaatataat tgttttcgaa ctgtaccgag
     1261 gcttgattgt ttatgagaga atcatttaca ttgtattcag gttgaaggat tgcatttgcc
     1321 tgaagtccca tataatcgtt ttgcgattta tcttgccatg cggtaacttt attatcggaa
     1381 acacttactt tttcagaagc gtcgatccaa aagcttatat cagcaggact aaagcgtttt
     1441 acaaaaaagc tatcaacgtc agtttcattg aaatcgttgc acatattaaa agcgaaaacc
     1501 ctccagtaat acgtgccggt atcagcaatt gcttgtgaat agctgttgtc ggtaatatca
     1561 gaactccata taatgttttc gaacccggca tcatcgctaa tttcgatacg ataatacaat
     1621 gtatttgaac ttggtgtcca actgaaaata accaacggat tcaaattgta gaaatatgct
     1681 tctgaagcag gggtaaggca atgaaaatcc tcgggtaatt ggaaatctgt atctacattt
     1741 aaacttagca ttgtactgtc gctgcagccg ttactgttgg ttactttttg ccagacttcg
     1801 aaagtcccgg atgttgtaaa gacgtgttgg gggttctctt catttgattc tgaaccatct
     1861 ccaaaatccc agtgccatga tgtgatttcc gggtcgttag cctcattgac aaatgaaaca
     1921 tccgaattca ggcaatgatg taaacccaga ttgacagaaa aagctgcttc aggattgggc
     1981 agaaccagta tttctttctg gataaagtta ttacacccgg catcggttga tgcgtacagt
     2041 tgcaccatgt aaccgcccgg tgcgtcatac gtatgaaccg gattttcttc cgatgatgtt
     2101 tcggagtcac caaaatccca actccactcc gatatttcga acggtggagt aattgtgcta
     2161 agattggtaa aatgggtttc ttccccaagg caagccgtgt ctgcgtcaaa gtcaacaacg
     2221 ggagcatatc ccgaaatgct tatttgtaca gtatctattc ccaggcatcc cctgttgttg
     2281 gtaacggtta catgataatt gcccgattcc gttgcaggaa ttaccgatga ggtagtgcca
     2341 tcgttccaca ggtagctcac aattcctgta ggtggtgaaa tcagcccaat gtttgttcca
     2401 tagcattcat cacggtcggc ccctagcgaa atctgatttg ggaaattatc aacttgtaca
     2461 ttgattatgt cgctggttgc ccagcatccc aataaatcat aaactgtaac tctgtagttg
     2521 ccttcgggat atgggccccc gatagttaac gcactacctc cgttatatcc agtgttccat
     2581 tcgtggtaga atgatcccag gccggtttcc catacgattt catcaccagc acaaatggta
     2641 gt
//
LOCUS       DAOOQP010000212         2635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_47180_length_2635_cov_6.556589, whole genome shotgun sequence.
ACCESSION   DAOOQP010000212 DAOOQP010000000
VERSION     DAOOQP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47180_length_2635_cov_6.556589"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLK75_12870"
     CDS             complement(<1..560)
                     /locus_tag="PLK75_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOY40183.1"
                     /translation="MDDANLLFAIGLTVFAGMATGIGSIMSLLSKKFNPKFLAASLGF
                     SAGVMIYVSLVEIFSKAKDSLVSEHGAQTGNIYTVVAFFTGIAVIALIDKLIPSFENP
                     HEIKNLEAKNLNPANSKKLLRIGLFTALAIAIHNFPEGLATFMGAINDPALGISIAVA
                     IAIHNIPEGIAVCGGIYLNKKNRKKA"
     gene            complement(815..1417)
                     /locus_tag="PLK75_12875"
     CDS             complement(815..1417)
                     /locus_tag="PLK75_12875"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOY40184.1"
                     /translation="MDLIQPLNVGIFLYDEVEVLDFAGPYEVFSTTMRNKENLFNVKT
                     ISENGNAIYARHGLKVLPDLSLQEANNLDILIIPGGCGVDEIENFNKEVFQWIKIQKR
                     KLKILASVCTGAFLLAESGLLNGRKATTHWMDIQRLQNDYPQIEVLRHVRFVDEGDII
                     TAAGISAGIDMSLFIVAKLFGYSIAANTAKRMEYAIDFKI"
     gene            complement(1602..1862)
                     /locus_tag="PLK75_12880"
     CDS             complement(1602..1862)
                     /locus_tag="PLK75_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40185.1"
                     /translation="MPLPKPHNCRPYCAACCTEPSISSPIPGMPKGKRAGERCIHLTT
                     ELLCDLIDSSDRPEVCTNFNFDPIICGDNREEALRIMAELEG"
     gene            complement(1881..>2635)
                     /locus_tag="PLK75_12885"
     CDS             complement(1881..>2635)
                     /locus_tag="PLK75_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOY40186.1"
                     /translation="LLATFRYGNAIKNGIPVTIAGKPNTGKSSLLNMLVNEDRAIVSN
                     IPGTTRDTIEEQAVIDGYFFRFIDTAGIRHTSNEIEIIGIERARKRIALSEIILLVAD
                     SGQTDAEIIESTNEIQALMDISAQHLIVLINKTDLSPERNYCLPFEVIPISATTGINI
                     EVLKTKLVQIVEKMKVAESDVIITGMRHAEALQNTKEALLRAQVGLYDGLPKDLLAQD
                     VRDAIHFLGAITGEINANEVLSSVFKNFCIGK"
BASE COUNT          749 a          572 c          492 g          822 t
ORIGIN      
        1 aatgcctttt ttctgttttt tttatttaaa taaattcccc cacagacagc aataccttca
       61 ggaatattat gaatagcaat ggctactgca atacttatcc ccaaagcagg gtcgtttatt
      121 gctcccataa aggtagccag accttccgga aaattgtgaa tggctatagc cagtgccgta
      181 aaaagaccaa ttctcaatag ctttttgcta tttgcgggat tcaaattctt cgcttccagg
      241 ttttttattt catgagggtt ctcgaacgat ggtataagtt tgtcgatcag tgctataact
      301 gctattccgg taaaaaatgc aaccacggtg taaatattcc cggtttgcgc cccatgctcc
      361 gaaaccagcg aatctttagc ttttgaaaat atctctacaa gcgaaacgta aatcattaca
      421 ccagccgaaa agcctaacga agcagctaaa aatttagggt tgaatttttt cgaaagcaac
      481 gacatgatgc taccgatacc agttgccatc cctgcaaaaa cggtaagccc tatagcaaac
      541 agcaagttcg cgtcatccat tgtcctataa aattagcaaa gcaatttata aattaaaacg
      601 gtacatttac tcatatgatt ataattttta catcatcgtt atgtaactaa atccacatgc
      661 atttgccgga aataagtaat tatatattct aatgaatagt gctcaacccg cacaaaagcc
      721 atctcgtttt ttgaaaacaa gcttgccatt ggaaaatctt atttatgcag gttaaaccgg
      781 agctgtcaat tgccagttgt tttcatacga ataattaaat cttgaaatca atggcgtatt
      841 ccattctttt cgcagtatta gctgctatag agtacccaaa aagttttgcc actataaaaa
      901 gcgacatgtc gatacctgca gatattccag cagcagtaat aatgtctcct tcatcaacaa
      961 accggacatg tcgtaaaacc tcaatttgag gatagtcgtt ctgcaatcgt tgaatatcca
     1021 tccaatgggt tgttgctttc cggccattga gtaagcctga ctctgccaac aagaaggctc
     1081 ccgtgcagac agaggccaat atttttaatt ttcttttctg aattttaatc cattgaaaca
     1141 cttccttatt aaaattctca atttcatcga caccacaacc tcccggaatt ataagtatat
     1201 ccaggttatt agcctcttgc aggctaagat cgggcagaac tttcaggccg tgccgggcat
     1261 aaatagcgtt accgttttca ctgattgttt tcacattgaa taggttttct ttattacgca
     1321 tggtggtgga aaaaacttcg tatggtccgg caaagtcgag cacttcaact tcgtcataca
     1381 aaaatatgcc tacattaagt ggttgtatta aatccattgt tttttaatca tgaaggcaat
     1441 ttataaaact tagaaataac ttctactttc aataggttat ttttttgttc attgaacgag
     1501 tgacaacagc atgcagggat tgggcatgat ttcgttttaa atagttccat tagtcgaatt
     1561 tatttctaca gggcatttca ttgcaagatc tcgctgtatt ttcagccttc aagttcggcc
     1621 atgatgcgca gtgcttcttc gcgattgtcg ccgcaaataa ttgggtcgaa attgaagttg
     1681 gtgcacactt cagggcggtc ggatgagtct ataaggtcac agagcaattc agtagtcagg
     1741 tgaatgcacc gctctcctgc ccttttcccc ttgggcatac ccggaatcgg tgaggaaatc
     1801 gatggctctg tgcaacaggc agcacaataa gggcggcaat tatgtggttt tggtaagggc
     1861 atggtagttc ttctgatttt ttatttaccg atacaaaaat ttttaaacac actgctcaac
     1921 acctcatttg cgttgatttc accggttata gctcccagaa aatggatcgc atcacgtaca
     1981 tcctgtgcaa gcaagtcttt tggcaaccca tcgtacaaac ctacctgggc ccgcaacaat
     2041 gcttctttcg tgttctgaag tgcctcagca tgacgcatac cggttatgat aacatcactc
     2101 tctgcaactt tcattttttc cacgatttgc accaattttg tttttagtac ttcaatatta
     2161 attcctgtgg ttgccgaaat ggggattacc tcgaacggca aacaataatt acgctcagga
     2221 ctcagatcgg ttttgttaat aagcactatc aaatgttggg cactaatatc cattagcgct
     2281 tgaatctcgt ttgttgactc aataatttca gcatcggtct gacccgaatc ggcaacgagt
     2341 aaaataatct ccgacaaagc aattctcttt cgggctcgct ctatcccaat aatttcgatt
     2401 tcgtttgatg tgtgtcgtat tcctgctgta tcgataaacc ggaagaaata gccatcaata
     2461 actgcctgct cttcgatagt gtcgcgtgtg gttcccggaa tattgctgac aatagccctg
     2521 tcttcattta ccagcatatt cagcaacgac gacttaccgg tatttggttt tccggctatg
     2581 gttacaggga taccattttt tatggcattc ccataccgaa aagttgccag taatt
//
LOCUS       DAOOQP010000213         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_53271_length_2420_cov_4.701057, whole genome shotgun sequence.
ACCESSION   DAOOQP010000213 DAOOQP010000000
VERSION     DAOOQP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53271_length_2420_cov_4.701057"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PLK75_12890"
     CDS             complement(<1..1419)
                     /locus_tag="PLK75_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40187.1"
                     /translation="MKKCPIIFLLLLCFGLCPELSTAQTWKELDSLSHELQYKRYYSD
                     AAEYAEDALQSAAKELGKTDTVYAYLLNRAMEVNTLSGNNKEAVKYGNAELSLRENTL
                     KQSKKKLVDCKIDLALAYTGAEDFTNAKKIIDDLLLFAATEEGESSLLYAQVCIGTGR
                     VYLRNKDYENAETWLQKAKSIFEKQSVLNSGSYAQALLFMARVYIDSRNEEKYDTAEM
                     LLLNSILITEKNFGAYSFQYKTNVDVLYDFYYYKYSRAPTRIYYDWKKLIASSPQNAL
                     GLKLTLIEEYFSYEVTKYDDYFEPILQEIYDSLSKTETKNPEQYALLCYLLCKVYTYQ
                     GDFEKAFAYCSEGLEVLEEISKRVIPSYASDFYKLATILYHGQGNFSTAIQSYLNSNN
                     VFLKQFDFSFDDLSCKFSMNLCSDLKAYFKQFTYDNAVFYYKLIEADKLLEYARYNSS
                     QKINYLKGALDNYESSCNLLKKN"
     gene            complement(1921..2127)
                     /locus_tag="PLK75_12895"
     CDS             complement(1921..2127)
                     /locus_tag="PLK75_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40188.1"
                     /translation="MGKLHLINVLRDIDNAETYKREVDGLFAAMKQLNLKESLLITDY
                     QEKEKIIEETVIHIVPLWKWLINK"
BASE COUNT          719 a          478 c          417 g          806 t
ORIGIN      
        1 gttctttttc aacaagttgc aactcgattc gtaattatca agtgcgcctt tcagataatt
       61 tatcttctga gaggaattgt atctggcata ttccagcagc ttatcagctt cgatgagctt
      121 ataataaaag actgcgttgt cgtatgtaaa ctgtttaaaa taagctttca ggtcggaaca
      181 tagattcata ctgaatttac agctcaagtc atcgaagctg aaatcaaatt gcttcaggaa
      241 aacattattt gaattcaaat aagattgtat tgctgttgaa aagttgccct gtccatgata
      301 caggattgtt gccagtttat agaaatcgga tgcataagag ggaattacac gttttgaaat
      361 ttcttctaga acttcaagcc cttcggaaca atatgcaaag gccttctcaa aatcgccctg
      421 ataggtataa accttacaga gcaaatagca tagaagtgca tattgttcgg gattttttgt
      481 ttcagttttg cttaatgaat catatatttc ttgcagtatg ggttcgaaat aatcatcgta
      541 tttcgtaact tcataactga agtattcttc gataagggtc aattttaacc ctaatgcatt
      601 ttgcggtgat gaagcaatca gctttttcca gtcgtaataa atgcgagtcg gagcccttga
      661 atatttatag taatagaaat catacaaaac atccacgttt gttttatatt gaaaagaata
      721 tgcaccaaag tttttttcag ttatcagaat tgaatttagt aaaagcatct ctgcagtgtc
      781 gtacttttct tcgttgcggg aatcaatata tacacgcgcc atgaatagca atgcttgcgc
      841 atagctgcct gaattgagta ccgattgctt ttcgaaaatc gacttagctt tttgcaacca
      901 ggtttccgca ttttcataat ctttattcct taagtaaacc ctaccggttc caatgcaaac
      961 ctgagcgtaa agcaacgagg actcgccttc ttccgtagca gcaaaaagca gcaaatcgtc
     1021 gattattttt tttgcgttcg taaaatcttc agcaccggtg tatgccaatg ccagatcgat
     1081 cttacaatcg acaagtttct ttttcgattg cttcaaggtg ttttcacgca ggctcaattc
     1141 agcgttacca tatttgacag cctctttgtt attccctgaa agcgtattta cttccatggc
     1201 tcggttcaac agatacgcat aaacggtatc ggtttttcca agttcttttg ctgctgattg
     1261 caacgcatct tctgcatact ccgcagcatc gctataatac cttttgtact gtaattcatg
     1321 tgataatgaa tctaattctt tccatgtttg tgcggttgat aactcaggac aaagtccgaa
     1381 acaaagcaat aacagaaaaa tgatcgggca ttttttcatg gcagagattt tgggtttccc
     1441 aaatataata aaaacgactt aaaatgaaaa attattaaaa cctatgatcc tcattatcac
     1501 aatatctggt tcagttcgcc agattggatt cagtaagcta taatctacca caccctccag
     1561 tcaaactcca gattctccta ccggctggtg aagcgctggc ggccgttggt tgcagttgat
     1621 aattcagcta caaataatca gaaaagttat cggggtttac aacatcgaat gtggcatcgg
     1681 ggtatgcact attttttgat tatttttttc gaaatacgag catcgcgata catgtattta
     1741 cataaattca ggtagctaaa atgcattaat tttgaagtct gacatagtat aagatttttt
     1801 atgtctgtat tatggtttga ttaatttgca aattcaatac atcctgcatt caaactctaa
     1861 attcacatac ctactgcttg cattaaagca aattatggac taaacacagc caagaatacc
     1921 tcacttatta ataagccatt tccaaagtgg cacaatgtgt ataactgtct cttcaataat
     1981 tttctctttt tcctgatagt ctgttattaa taaactttct ttcagattga gttgtttcat
     2041 agcagcaaat aaaccatcca cttctctttt ataggtttcg gcattgtcga tatcacgtaa
     2101 tacatttatc agatgaagtt tgcccaaaga cttaatcacc tcctaaatgc cgcgtaatct
     2161 attttaaaag tccttttctc atttgttttt ttctataaat acaaaaaggg gcatatatat
     2221 ccctataaat agatacaaat aatttttgat tggcatgttt ttataaaact atttcaatat
     2281 gtgaacaatc attccctacc acaccctcca gtcaaactcc agattttcct gccggcaggt
     2341 ggggcgctgg cgaccgttgg tgagacggtc cacctcaacg attacgtagc tgcgaatttc
     2401 gctcaggctt acctgcttgt
//
LOCUS       DAOOQP010000214         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_54462_length_2383_cov_4.261598, whole genome shotgun sequence.
ACCESSION   DAOOQP010000214 DAOOQP010000000
VERSION     DAOOQP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54462_length_2383_cov_4.261598"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PLK75_12900"
     CDS             complement(<1..1475)
                     /locus_tag="PLK75_12900"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HOY40189.1"
                     /translation="MMNKTNSPVAPVATKHVTTLSMHGHSRTDKYYWLNDRNNPAVIE
                     YLEAENNYLNTMMAHTALLQSKLYVEMIDRIKPEDASVPYIRNGYYYYIRYEEQKEYP
                     VYCRKKDVSGAQVEILLDGNEMAKNYSYFQIGDFSVSPDNTILAFSTDNVGRRLFAIN
                     FRNLVTGETYSETIPDTDGSIEWANDNHTIFYCLKDTVTLRAFEVKKHRLGSGINTDS
                     TIFTESDETFELSIGKTKSGKFITIESGSTLSSEIWFLDCNATEEKPKVFQPREKEHE
                     YTVDHAGKEFYIVTNWNARNFRLMKCSEKNTSKENWVEVIPHRMNVMLESFELFNDFV
                     VVNERSDGLNLFRVLSRDDNSSYYIDFTEEVYDIGFAENCESNSVKLRFQYSSLTTPA
                     SVFDFELKTKEKTLLKEQSVLGNFDKNNYETKRLYAKSKDNKNIPITIVYRKGISLNG
                     KNPLLLYGYGSYGYTIDPTFSAARLSLLDRGFIFAIAHVRGG"
     gene            complement(1653..>2383)
                     /locus_tag="PLK75_12905"
     CDS             complement(1653..>2383)
                     /locus_tag="PLK75_12905"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40190.1"
                     /translation="WIDGNRDGEMIATNYTFLYNTNITSMDIYIGSQTTAGTQIIGHV
                     MEYNDADETWVDISATALYEIQENVLGSWVNLTFTDPVAIDLAGEDGKSVKAAIEFYY
                     GSTSNKVWIGVDPTVKDSFWGVSWFLVTGSSANTWYSFTNWANGGLGVRLLTDDADLT
                     SVTPVATDNTTIYPNPSNGIINIDNIEGASVEVMNVLGQTVESIEIASAHNTIDMSSY
                     AAGTYVVRIVNGANVTTHKVNITK"
BASE COUNT          653 a          478 c          468 g          784 t
ORIGIN      
        1 cctccccgca catgggcaat ggcgaaaatg aagcctcggt caagaagact tagtcttgct
       61 gctgaaaaag tcgggtcaat agtataacca taactaccgt agccgtatag caacaatgga
      121 ttctttccgt tcaggctgat tccttttcga tacacgattg taatcggaat gttcttattg
      181 tctttcgatt ttgcatacag ccgttttgtt tcatagttgt ttttgtcgaa atttcctaat
      241 accgactgct cctttaacaa ggttttttct ttcgttttca gttcaaaatc gaaaactgat
      301 gcaggtgtgg taagcgaact atattgaaat ctgagtttta ctgagttgct ttcgcaattt
      361 tcggcaaaac cgatatcata tacttcttcg gtgaaatcga tataatacga cgaattatca
      421 tctcttgaaa gtaccctgaa cagattgagc ccatcggacc gttcgttcac cacaacgaag
      481 tcgttgaaca gttcaaaaga ttcaagcata acattcatgc ggtgcgggat tacttcaacc
      541 caattttctt tggatgtgtt tttttcagag catttcatta atctgaaatt tcttgcattc
      601 cagtttgtta cgatataaaa ctctttgcca gcatgatcga cagtatattc atgctctttt
      661 tcgcgaggtt gaaaaacttt gggtttttct tctgttgcgt tgcagtccag aaaccagatc
      721 tctgatgaca aggtacttcc tgactcaatt gtaataaatt tccccgattt ggttttccct
      781 atcgataatt caaacgtttc atctgactcg gtaaaaattg ttgaatcggt attaatgcca
      841 gaccccagtc tgtgtttttt gacttcgaaa gctcggagag taactgtatc ttttaaacag
      901 tagaaaattg tgtggttgtc gtttgcccat tcaatactcc catcggtgtc gggtattgtt
      961 tcggaataag tttcaccggt aacgagattc ctgaaattga ttgcaaacag tctgcggccc
     1021 acattatcag tactgaacgc taaaatcgta ttgtcagggc ttacagagaa atcaccgatt
     1081 tggaaatagg aatagttctt tgccatttcg ttcccatcaa gtagaatttc tacctgagcc
     1141 cctgaaacat ctttttttct gcagtacacc ggatactctt tttgctcttc gtatcgtata
     1201 taataatagt acccatttct tatatatggc actgaggcat cttctggttt aatccggtca
     1261 atcatttcta cgtagagctt cgactgaagt aaagcagtat gcgccatcat tgtatttagg
     1321 taattgtttt cagcctccag atattcgatt acggcagggt tattccggtc gtttaaccaa
     1381 taatatttat cggttcttga atggccatgc atgctcaggg tagttacgtg ttttgttgct
     1441 accggagcaa caggagaatt ggttttgttc atcataccgg aattcattga aatgactgat
     1501 taattatggt taatgtaggc tgtaatttca tgatacagag ataggctgat aaaaatagtt
     1561 gaagttttaa agatgtgcaa ttatttgggg aacatacgaa aataaaaaaa gctgtccttg
     1621 ggagacagct tttagaaaaa ctgtgaaagt gtttatttcg taatattcac tttgtgtgtt
     1681 gtaacgtttg caccattaac aatacgaaca acatatgtgc ctgcagcata tgaactcatg
     1741 tcgattgtgt tgtgagctga agcaatttcg atgctctcaa ctgtctgtcc aagtacattc
     1801 ataacttcta cacttgcacc ttcgatattg tcgatattga taataccatt acttggattt
     1861 gggtaaatag ttgtattgtc ggtagcaacc ggagtaactg atgtaaggtc agcatcatcg
     1921 gtcagcagac gtacaccaag tccaccgtta gcccagttgg tgaatgaata ccatgtattg
     1981 gcactagatc ctgttacaag gaaccagcta acaccccaaa aactgtcttt aacagttggg
     2041 tcaacgccaa tccatacttt attgcttgtc gatccgtaat agaattcaat ggcagcttta
     2101 accgatttac catcttcccc tgcaaggtca atagccaccg ggtcggtgaa tgtaaggttt
     2161 acccacgaac ccaatacatt ttcctgaatt tcgtaaagtg cagtcgcgga aatatcaacc
     2221 catgtttcgt cagcatcgtt atactccatt acgtgtccga taatttgagt tccggctgtt
     2281 gtttgagatc cgatataaat atccatgctg gtaatgttgg tattgtaaag gaatgtgtaa
     2341 ttggttgcaa tcatttcacc gtccctatta ccatctatcc agg
//
LOCUS       DAOOQP010000215         2284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_57948_length_2284_cov_3.934500, whole genome shotgun sequence.
ACCESSION   DAOOQP010000215 DAOOQP010000000
VERSION     DAOOQP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57948_length_2284_cov_3.934500"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1494
                     /gene="lpdA"
                     /locus_tag="PLK75_12910"
     CDS             103..1494
                     /gene="lpdA"
                     /locus_tag="PLK75_12910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOY40191.1"
                     /translation="MDFDILVIGSGPGGYVAAIRAAQLGLKTAVVEKAELGGICLNWG
                     CIPTKALLKSAQVFNYMKHSEDYGITIQGNAEPDFAKVIARSRAVAEGMSKGIQFLLG
                     KNKITVIKGFGKLKASNTVSVTDEAGQEKEYTSPHIILATGARSKELQGVKQDGLHII
                     GYREALSLQKKPASMIVIGSGAIGTEFAWFYHSMGTEVTLVEYLPNLVPLEDEEVSKQ
                     LERSFKKAGIKVLTGTAVENAVFNGNSCEVHVNTKKGQDVLHAEVVLSAVGVTTNLEN
                     LGLEALNIANEKGKILVDEFYRTNVEGIYAIGDIVHGPALAHVASAEAICCVEKIAGQ
                     HAEPVNYSNIPSCIYATPEIASVGMTEKQAIEAGFEVKIGKFPYSASGKASAAGNKDG
                     FIKLVFDAKLGELLGAHFIGDNVSEMIAEMVVARKLEATGHELIKSIHPHPSMSEAIM
                     EAAAAAYGEVIHL"
     gene            complement(1545..>2284)
                     /locus_tag="PLK75_12915"
     CDS             complement(1545..>2284)
                     /locus_tag="PLK75_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY40192.1"
                     /translation="SPVTVAISTTDVTCNGERNGAINLIATGGTLPYAYHWQYNGFES
                     AQQQLTSLQAGIYSFTVTDANHCELDSMATISEPSEMVVTYISTNPSCIGADDGYIEI
                     VASGGTFPYMYNWYQGSSDLPYIMGLIQGNYLITVVDANNCIRIIEQIVLTDDPIDCI
                     RIPNAFTPNNDGVNDTWIIENIDMFPAAVVQVFNRWGQKLFEAKGVDEPWNGYYDGKL
                     LPTGSYLYAVRLFNGEEPYVGIVTLVK"
BASE COUNT          690 a          468 c          517 g          609 t
ORIGIN      
        1 aaatccatgt cccaaattta aaactttcca ctaaatttgc agtagaacca ataatattaa
       61 ggaattcaaa aaacatcgta aattcgcata aaaatatata ctatggattt tgatatatta
      121 gttattggaa gcggacccgg tgggtatgtt gctgcaatcc gtgctgcgca actgggttta
      181 aaaactgctg tggttgaaaa agctgagctg ggtggcattt gcctgaactg gggatgtatt
      241 ccaaccaagg ccctgcttaa aagcgcccag gtatttaatt acatgaagca ctccgaagat
      301 tatggtatta caattcaagg gaatgcagaa cccgattttg ctaaggtaat agcccgcagt
      361 cgtgctgttg ccgaaggtat gagtaaggga attcagtttc tattaggtaa aaacaaaata
      421 accgttatca aaggttttgg aaaattgaaa gcaagtaaca ccgtttctgt aaccgatgag
      481 gccggccaag agaaagagta tacttcgccg catattattc tggctaccgg agcacgttca
      541 aaagagttgc agggagtgaa acaggacggg ttacatatta ttggataccg tgaagcacta
      601 tccctccaga aaaaacctgc ttcaatgatc gttataggca gcggtgctat tggaaccgaa
      661 ttcgcctggt tctaccatag tatgggtact gaagttactc tggtggaata tctcccaaat
      721 ttggtccctc tcgaagatga agaagtttca aaacaactgg aacgctcctt taaaaaagca
      781 ggtataaaag ttttaacagg aactgcagtt gaaaatgctg tatttaatgg gaatagctgt
      841 gaggttcatg tgaatacaaa aaagggtcag gatgttcttc atgcggaagt tgtgctttcg
      901 gctgtcggag taactacgaa tctcgaaaac ctggggcttg aggctcttaa tatcgccaat
      961 gaaaaaggaa aaattctggt tgatgaattc taccgtacca atgtggaagg tatatacgcc
     1021 attggtgata tcgtgcacgg acctgcgctg gctcatgtgg cttcggctga agctatctgc
     1081 tgtgtcgaga aaattgcagg gcaacatgca gagcccgtga attacagcaa tattccttca
     1141 tgcatttatg caactcccga aatagcatcg gttggtatga ccgagaaaca agcgattgaa
     1201 gctggttttg aagtaaaaat cgggaaattc ccctactcag cctctggtaa agcatcagca
     1261 gccgggaata aagatggttt tatcaagctg gttttcgatg caaaactggg tgaactcctt
     1321 ggcgcccatt ttataggtga caatgtaagc gaaatgattg ctgaaatggt agttgcccgt
     1381 aaactcgaag caacgggtca cgaacttatt aaaagcatac atccgcaccc gagtatgtcg
     1441 gaagctatta tggaagcggc agctgcagcc tatggtgaag tgatacacct ataataaggg
     1501 tgcttagcat ttcgatccct cagggtaacc gttatttctg cagcttattt aactagggta
     1561 actataccca catagggttc ttctccgtta aacaggcgca ctgcgtacaa gtaggaacct
     1621 gtaggaagaa gtttaccgtc ataatatcca ttccatggct catccacccc ctttgcctca
     1681 aatagcttct gcccccaacg attgaacacc tgaacaactg ctgccggaaa catatcgata
     1741 ttctcgataa tccatgtatc gtttacacca tcgttgttcg gggtaaaagc attggggata
     1801 cggatacaat caataggatc atcggtgagc actatctgtt ctatgattct aatacaattg
     1861 tttgcatcaa ctaccgtaat caggtaattc ccctgaataa gccccatgat atatggcaaa
     1921 tcggaactgc cttggtacca attgtacata tacggaaaag ttcctccgga tgcaacaatt
     1981 tcaatataac cgtcatcagc accaatacag gacgggttgg tgctgatgta ggttacaacc
     2041 atttcagaag gttccgatat ggtcgccatt gaatctaact cacaatgatt ggcatctgtt
     2101 acagtaaagg agtatatacc agcctgcaat gaagtaagct gctgctgtgc agactcgaag
     2161 ccattatatt gccaatgata tgcataaggt agcgttccac cggttgcaat cagattaata
     2221 gctccattcc gttctccatt acaggtaaca tcagtcgttg aaatggccac tgtaacgggg
     2281 gaaa
//
LOCUS       DAOOQP010000216         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_58283_length_2275_cov_5.633784, whole genome shotgun sequence.
ACCESSION   DAOOQP010000216 DAOOQP010000000
VERSION     DAOOQP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58283_length_2275_cov_5.633784"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLK75_12920"
     CDS             complement(<1..554)
                     /locus_tag="PLK75_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOY40193.1"
                     /translation="MNSVIKFVFKNKTVEIDFEKNQCFNPSITLLDYLRLTGNSKGVK
                     EGCAEGDCGACTVVIAETDKNGRLHYKAVDSCLMFLPMIHGKQLICVEDLVLAENGRQ
                     QLHPVQQCMVESDATQCGYCTPGFVMSVFALFKNNNNPTEQEIVNALSGNLCRCTGYQ
                     PIIDAAKKACVYNGIDHFSDDEKKV"
     gene            complement(625..>2275)
                     /locus_tag="PLK75_12925"
     CDS             complement(625..>2275)
                     /locus_tag="PLK75_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY40194.1"
                     /translation="SLQNLRVSRSRYSDVTISVGSSSDDIRYQNTAPGLYAASLYSVA
                     MDSAKNISEHEQQSMKIDWTAPAAVGNLCDGSAADIDLTQNLTQLSANWSASTDVNSG
                     ITAYYYAIGTSPGGTQLVNWTANGTNTSFTKTGLSLVPETTYYISVKALNGAGLYSDV
                     SVSDGVKAIVVPVCPDDISVCADAAPFALSGASPAGGSYSGINVSGGVFNPASAGVGE
                     YLIYYSLGSQSCSFIVSVNPLPVMDCPDNIYITINDDAFTLMEAYPSGGQFYYNDEQI
                     TVFDPAAFGVGNFEIFYKYITPQSNCQGVCSFFIFVYSDTQVTCPEDFGVCIGDSPFD
                     LEGATPPGGTYYLYSEIVSVFDPSYWGIDEHVVEYVYNDASCQFTITVNGLPEVICPL
                     DITLSQDDDPITLTGGMPEGGWYIVGWISATEFDPALYEPDYYDVTYNYYDPETGCFD
                     FCLFGITVTQGVFSNTQSTGWFHLSPNPNNGIFSISLNKTALPIKIVIYSINGKIMYE
                     SNEICEFTGNTINFTSKLLPGMYFVKISNSEGSYTTKMVVE"
BASE COUNT          711 a          516 c          415 g          633 t
ORIGIN      
        1 acttttttct catcatcaga aaagtgatct ataccattat agacacaagc ttttttagca
       61 gcatcgatta ttggttggta accagtacag cgacataaat ttcccgataa agcatttaca
      121 atttcctgtt cagtaggatt gttattgttt ttaaacaagg caaaaactga cataacaaaa
      181 ccgggagtgc aataaccaca ttgtgttgca tcactttcaa ccatacattg ctgcacgggg
      241 tgaagctgtt gcctgccatt ctccgctaaa acaagatctt ctacgcaaat taattgctta
      301 ccatgaatca taggtaaaaa catcaaacat gagtcgacgg ctttgtaatg caatctgccg
      361 tttttatcag tctcagcaat caccacagtg catgcgccac aatcaccttc agcacagcct
      421 tctttgactc cttttgaatt tccggttaaa cggagataat ccaatagagt gatgcttggg
      481 ttaaagcact gattcttttc aaaatcaatc tcaacggttt tgttcttaaa aacaaatttt
      541 ataacgctat tcatgtgcat tatttcttga caaaaatact ataccgtata catcaggcat
      601 tattacaaga tatccaaata agtcttattc tacaaccatt ttcgtcgtgt aactaccttc
      661 cgaattacta attttcacaa aatacattcc cggtaacagc ttactggtaa aattgatagt
      721 attcccagta aattcacaaa tttcatttga ttcgtacatt attttaccgt taatgctata
      781 aatcactatc tttatgggaa gtgctgtttt attcaatgat attgagaaga tcccattgtt
      841 tggattcggg gataaatgaa accatcccgt gctttgtgta ttagaaaata caccctgagt
      901 tacggtaatt ccaaataaac agaagtcaaa acaacctgtc tctggatcgt aataattata
      961 tgtaacatcg tagtaatctg gttcatacaa ggcaggatca aattctgttg ctgaaatcca
     1021 tccaactata taccaaccac cctcaggcat accgcctgta agcgttatag gatcatcatc
     1081 ctgcgataat gtaatgtcga gaggacagat aacctccgga agcccgttca cagtaatggt
     1141 aaattgacag ctagcgtcat tatacacata ttcaaccaca tgttcatcaa taccccaata
     1201 acttggatcg aaaactgaaa ctatttcgga ataaagataa tatgtacctc cgggaggagt
     1261 agcaccttca aggtcgaaag gactatcacc aatacacaca ccaaaatctt cgggacatgt
     1321 tacctgagta tcggaataaa caaaaatgaa aaatgaacaa actccctgac agttgctttg
     1381 gggagttatg tatttataaa atatctcaaa atttccgact ccgaaagccg cagggtcaaa
     1441 cactgtaatc tgttcatcat tataataaaa ctgaccccct gagggatatg cttccatcag
     1501 agtaaaggca tcatcattaa ttgtaatata aatattgtca gggcaatcca tcacaggcaa
     1561 cgggttcact gaaacaataa agctgcacga ttgagaaccg agtgagtagt atattaagta
     1621 ttccccgaca cctgcagagg ccggattgaa aacaccccct gaaacattta ttccgctata
     1681 gcttcctcca gcagggcttg caccactcaa tgcaaatggt gccgcatcgg cacaaaccga
     1741 aatatcatcg gggcaaacgg gtacaacaat cgcctttacg ccatccgaaa cagaaacatc
     1801 actatacagc cctgccccat ttaacgcctt tacgctgata tagtatgtgg tttcgggcac
     1861 aaggctcaga cccgtttttg taaatgaagt gttcgtacca ttcgctgtcc agttcaccaa
     1921 ttgcgtacca cccggactgg tcccgattgc ataataataa gctgtaattc ctgagttcac
     1981 atcggttgag gcagaccaat tcgccgataa ctgggtgagg ttttgcgtca ggtcaatatc
     2041 ggcagcagag ccatcgcaaa gatttccaac tgccgccggg gctgtccagt caattttcat
     2101 ggactgctgt tcgtgctccg aaatgttttt ggcggaatcc attgcaaccg aatacaggct
     2161 ggctgcatat aatccaggag ccgtattctg gtagcggata tcgtccgaag agctgccaac
     2221 actaatggtt acatcagagt aacgacttcg cgacaccctc aggttctgca atgaa
//
LOCUS       DAOOQP010000217         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_59150_length_2252_cov_5.654529, whole genome shotgun sequence.
ACCESSION   DAOOQP010000217 DAOOQP010000000
VERSION     DAOOQP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59150_length_2252_cov_5.654529"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="PLK75_12930"
     CDS             complement(<1..1602)
                     /locus_tag="PLK75_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40195.1"
                     /translation="MKKTSSMLKVVTLLGFTFFLVIIGAKDVFSQCANNSAGTIYPNA
                     SWQYTGYYGSMPYWTFTGVAGGVYSFSNCDATAEDTYMRVYDASWNQLAYNDDYGPYC
                     SSSNASLDWTCTSGGTYYVAICHYSCSNLSNTQRLTYKAVIPEMTVPTSSYNSYTTCS
                     GHLYDSGGSAGGYNNSQDGYTIIYPGSSNMKAQLTGSYTTESGWDYVYIYDGAGTGGT
                     LLFSGSGSGSIGTIVSSGANVPLTVRFTSDGSVTSTGFDFTISCVCVPSAAGVYGSGS
                     WLSYGYNGQDLNTYVGRITGITQTWNNLSANWGSNGATTECGSTNTDYFSTRHMMNYN
                     FTAGIYSIYYSTDDGVRISTDGGSSWNIVNAWDNTTHSGTVYVYLSGSTNIIADQREN
                     AGGAAITVTITPQCYISPTYGSSVWNTYGYNGSDLNTLYGQVTGITNTWNNLSGSWGS
                     GAPTTNCLSMPADNFSTRSMMNYNFTSGYYQFTYSSDDGIRISTNSGASWNVINYWDN
                     TTHSGSIMVYLSGSTNLIVDQREIAG"
     gene            1716..2015
                     /locus_tag="PLK75_12935"
     CDS             1716..2015
                     /locus_tag="PLK75_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOY40196.1"
                     /translation="MKKSRRKFSASFKAQVAIAALQERESLAELSERYGVHPNIISKW
                     KQEFLKNSARVFDKDEGQGNSAEIEKLYSKIGKLQVERDFLEKVYLMSGLGKSSK"
     gene            2012..>2252
                     /locus_tag="PLK75_12940"
     CDS             2012..>2252
                     /locus_tag="PLK75_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOY40197.1"
                     /translation="MMVKRNHKLSIRQQCELLEIPRSGLYYKPVKEQEENLHIMRLLD
                     EEYLKHPTHGVLRMQDFLLGLSIVVNHKKVRRLLRL"
BASE COUNT          705 a          473 c          442 g          632 t
ORIGIN      
        1 gcccgcgatt tccctctgat caacaattag gttggtgctt ccgcttaaat aaaccataat
       61 acttcctgaa tgggtagtat tatcccaata atttataaca ttccatgatg caccgctatt
      121 tgtacttatt cttataccgt cgtcggaact gtaggtaaat tgataatacc ctgaggtgaa
      181 gttataattc atcatagatc gggtgctaaa gttatcagca ggcatgctca aacaatttgt
      241 agttggggca cctgaacccc acgagccgct taaattattc catgtatttg taattcctgt
      301 tacctgacca tataaagtat ttaaatctga tccgttataa ccataagtat tccacacact
      361 gcttccataa gtcggtgaaa tatagcattg aggtgtaatt gttacagtaa tagcagcacc
      421 ccctgcattt tctctttggt ctgcaataat attggtactt ccgctgagat atacataaac
      481 ggttcccgaa tgtgttgtat tatcccatgc atttacgata ttccacgaag accctccatc
      541 agtactaatt ctcacaccgt catcagttga ataatagatt gaatagatgc cagctgtaaa
      601 gttataattc atcatatggc gtgtactgaa ataatcggtg ttggtcgatc cacattcagt
      661 agttgcaccg ttcgaccccc aattggcgga tagattgttc catgtctgcg taattccagt
      721 aattcttcca acgtaagtat tcaaatcctg accgttgtag ccatagctta accatgatcc
      781 agaaccatac actcccgcgg cagaaggaac acatacacaa gaaatagtaa aatcgaatcc
      841 cgttgaagta acagaaccat ctgaagtaaa cctaactgtt agcggaacat tggctcctga
      901 tgaaactatt gttccaattg aaccacttcc actaccgcta aaaagcaggg ttcctccggt
      961 tcctgctcca tcataaatgt aaacataatc ccagccggat tctgtagtat atgaaccggt
     1021 taattgagct ttcatatttg aactaccggg ataaataatt gtataaccat cctgagagtt
     1081 attgtaacca ccggctgagc caccgctatc gtataaatgt cccgagcacg tagtgtagct
     1141 attatatgaa cttgtaggaa cagtcatttc cggaatgaca gctttatagg ttaaccgctg
     1201 tgtattcgac aagttcgaac aactgtaatg gcaaatcgcc acataatatg ttccacccga
     1261 tgtacatgtc caatcgagtg aggcgtttga gctactgcag tacggtccgt aatcatcatt
     1321 ataggctaac tgattccatg aagcatcgta taccctcatg taagtatctt cggccgttgc
     1381 atcacagttt gaaaagctat ataccccccc tgcaacaccg gtaaaagtcc aatatggcat
     1441 agaaccataa tatccggtat attgccaact cgcattgggg taaattgttc cagcactgtt
     1501 gtttgcacac tgcgaaaaaa catccttagc accaataatt actagaaaaa aggtaaaacc
     1561 caatagggtc actaccttta acatcgatga agtttttttc atttgggaat ttttatatgc
     1621 cgtatcttta atggttttca tacaaatttt ataatactcc ccgaaatttg gaccactggt
     1681 taagttgaat aaaaacgatt aaatttacca gtattatgaa aaaaagcaga aggaagttta
     1741 gtgcaagttt caaggcacag gtagccattg cagcactcca agagcgagag agcctggccg
     1801 agttatcaga acgttacgga gtacatccga acataatcag taaatggaag caagagtttc
     1861 tcaagaactc tgcccgagta ttcgataaag atgaaggaca ggggaattcg gcagagatag
     1921 aaaaactata ttccaaaata ggcaaattac aggtcgagcg ggatttttta gagaaagtct
     1981 acttgatgtc cggtctggga aagagctcaa aatgatggta aaacggaacc ataagctcag
     2041 tatccgacaa cagtgtgagt tgttggaaat acccaggagt gggctttatt acaaaccggt
     2101 taaagaacaa gaagagaacc tgcatatcat gcgattgttg gacgaagagt atttaaaaca
     2161 tccgacccat ggggtgttac ggatgcaaga ttttctgttg ggattaagta ttgtcgtcaa
     2221 ccacaaaaag gttcgtcgat tactacgcct ga
//
LOCUS       DAOOQP010000218         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_75714_length_1911_cov_4.935345, whole genome shotgun sequence.
ACCESSION   DAOOQP010000218 DAOOQP010000000
VERSION     DAOOQP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75714_length_1911_cov_4.935345"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLK75_12945"
     CDS             complement(<1..355)
                     /locus_tag="PLK75_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY40198.1"
                     /translation="MELLQNKVVLITGGARGIGRSIAIAVAKQGAQVAFSDLRADENY
                     NRLLSELRSLGVKAEAFESNAASFTDAEKLINDVYAAFGRIDVLVNNAGITRDNLLMR
                     MTEEQWDMVINVNLKS"
     gene            497..1312
                     /locus_tag="PLK75_12950"
     CDS             497..1312
                     /locus_tag="PLK75_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOY40199.1"
                     /translation="MNADAILEKLVDIGIGYGPKLIGAIVILLLGLWIIKLFTKSLRK
                     MMNKRNTNKELRSFFLSLVNIALKVLLFVSVLGMVGIEVTSFIAILGAAGLAIGMALQ
                     GSLQNFAGGVIIILLKPFKVGDVLEAQGYLGIVKEIQIFSTILTTFDNKTIIIPNGGL
                     ATSSMTNYSTESLRRVDWLYQVAYGDDIDKVKALCTKLIENDSRILKEPAAFIAVNGL
                     KDSSVEVAVRVWVKSEDYWAVFHSMNENVYNTFRSEGLHLPYPQMDVHLHNSK"
     gene            1313..1744
                     /locus_tag="PLK75_12955"
     CDS             1313..1744
                     /locus_tag="PLK75_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY40200.1"
                     /translation="MATDSLSLQWVDGMEFTSLVNGHSITIDANSEFGGKNKGPRPKP
                     LMMLALAGCTGMDVISILHKMRVFPDSFKVNIETAITDEHPKHYTRMKVIYEFSGENL
                     PENKLKRAVELSEQQYCGVSAVYKKAMNLEFEIRLNNKVLA"
BASE COUNT          585 a          387 c          400 g          539 t
ORIGIN      
        1 ccgatttaag gtttacgttt ataaccatat cccattgctc ttctgtcata cgcatcagca
       61 ggttatcacg ggtaataccg gcattgttca ccagtacgtc gatgcgcccg aaggcagcat
      121 aaacatcatt aataagcttt tcagcatcgg taaatgaggc cgcattcgac tcaaacgctt
      181 cggcttttac tccgagactt cgcaattcag aaagaaggcg gttataattt tcatcagccc
      241 tcaagtcgga aaaagcaacc tgagccccct gctttgcaac tgcaatagcg atagatcttc
      301 cgatgcccct ggctccaccg gttattaaaa ccactttgtt ttgaagtaat tccatagtca
      361 gtaatttcag gtaaaaataa taaaatcaat ttcttttcta aaacattttc ccaggtatgg
      421 gataaacgca agtaatattg ctttaatatc tgcttttttt attaaattta cccaaataaa
      481 tcaaaactat atcgttatga atgcagacgc aattcttgaa aagcttgttg acattggtat
      541 tggctatggt ccgaaactta ttggagccat tgtaatcctt cttcttggtt tgtggattat
      601 caaactgttt acaaaatcgc tgcggaaaat gatgaataaa cgcaacacaa acaaagaact
      661 tcgttcattt tttctaagtc tggttaacat cgcgctgaaa gtactattgt ttgtaagtgt
      721 acttggtatg gtaggcatcg aagtaacatc gttcattgcc atattgggtg ctgccggttt
      781 ggctatcggt atggcacttc aggggagttt gcaaaacttt gcaggcgggg taatcattat
      841 cttgctcaag ccctttaagg tgggcgatgt gctcgaagcc cagggttacc ttggaatagt
      901 gaaagaaatt caaattttca gtaccatact tacaaccttc gacaacaaaa ccattattat
      961 accaaacgga ggtctggcaa ccagttccat gacgaattat tcaacagaat cgcttcgccg
     1021 tgtcgattgg ctctatcaag tggcctacgg cgatgatatc gacaaagtga aggctctatg
     1081 caccaagctg attgagaatg atagccgcat cttgaaagaa ccggctgcat ttatcgctgt
     1141 aaatggctta aaagacagct cggttgaagt tgctgtaagg gtttgggtaa aaagcgagga
     1201 ttattgggct gtgtttcatt ctatgaatga aaatgtgtat aatactttcc gaagcgaagg
     1261 tcttcatctg ccctaccctc aaatggatgt tcacttacat aattcaaaat aaatggctac
     1321 agattcttta tcactacaat gggtcgacgg aatggagttt acatctttgg taaacggcca
     1381 ttccattaca attgacgcca attccgagtt tggaggcaag aataaaggcc cccgtccgaa
     1441 accgctgatg atgcttgcct tagcaggatg taccggaatg gacgtaattt caattcttca
     1501 taaaatgcgt gttttccccg acagtttcaa ggttaacatc gaaactgcca tcacagatga
     1561 acacccaaaa cactacacac gcatgaaagt tatttatgaa ttcagcggcg aaaaccttcc
     1621 ggaaaacaaa ctgaaaagag ctgttgaact atcggagcaa caatactgcg gagtgagtgc
     1681 agtatataaa aaagcaatga atcttgaatt cgaaatcagg ttgaataaca aggtacttgc
     1741 atgaagcttt tattgatata ctgcagaagg ttttcgtatg acccggcagt taaaaccctt
     1801 gccgaagttg ctgatgaaac cgaagggaaa gaattcatca acgcgcaggt agcattcatt
     1861 caggtagaag aatcagatga aattaacagc catagtgtgg tggagaaact g
//
LOCUS       DAOOQP010000219         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_86288_length_1758_cov_5.578391, whole genome shotgun sequence.
ACCESSION   DAOOQP010000219 DAOOQP010000000
VERSION     DAOOQP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86288_length_1758_cov_5.578391"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLK75_12960"
     CDS             <1..546
                     /locus_tag="PLK75_12960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HOY40201.1"
                     /translation="HFIPKGTITWVQDKLDREFTPLEVMNMDGVYKDVLSKYTFRNNH
                     GNFVLCWDNARYTNHSFNSNCLTTAYDFEIAIRDIQPGEELTDDYGYLNLPVPMRAKP
                     EGTRRKVVHSDDIVKYYKVWDKKLISSFGDILKVAQPLRCLITDELWHKINRIAYGEE
                     HMDSILTCYYDEHHNSHSRLN"
     gene            complement(562..>1758)
                     /locus_tag="PLK75_12965"
     CDS             complement(562..>1758)
                     /locus_tag="PLK75_12965"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOY40202.1"
                     /translation="RTISWKRVMDMNDRSLRDVVIGLGGKAQGVPRETGFNITAASEV
                     MAILCLAESLKDLKERLGNIFIGYTYDNKPIFARDLKAEGAMAALLKDAIKPNLVQTL
                     ENTPAIIHGGPFANIAQGTNSIIATKMGLSLSDFVVTEAGFASDLGAEKFFDIKSQYG
                     GLNPNAVVIVATVRALKYHGGKSLSDLSQPDTIALNIGLANLDKHIENMQMFNFKPIV
                     AINRFASDSAEELQMIRKHCEEIGIRVAVNDAWEKGGKGALELAEIVAGEALSCRVCF
                     RPLYDFSWPVEQKIETIAKKMYGAAGVEYSLKARTQLKTITSLKLDKLPVCIAKTQNS
                     LSDDPKLKNRPHGFIVNIREIEIASGAGFIIPIAGEIMRMPGLPKNPSAELIDIDDNG
                     NISGLF"
BASE COUNT          475 a          405 c          365 g          513 t
ORIGIN      
        1 cactttattc ccaaaggtac tattacctgg gttcaggata agctcgaccg tgagtttaca
       61 ccacttgaag taatgaacat ggatggagtg tataaagatg tgctgagtaa atatactttc
      121 cgtaacaatc atgggaattt cgtattatgt tgggacaatg cacgttacac gaaccacagt
      181 ttcaattcaa attgtctgac cacagcctat gatttcgaga ttgccattcg cgatattcag
      241 ccaggagagg aactgacaga tgattacgga tatctgaacc tccctgttcc aatgagagcc
      301 aagcccgaag gaacacgacg caaagttgtg cattccgacg acattgtgaa atattataag
      361 gtttgggata aaaaacttat atcaagcttc ggcgatattc ttaaagttgc ccagcctctg
      421 cgatgcctta ttacagatga attatggcat aaaatcaatc gaatcgcata cggtgaagaa
      481 catatggata gtatactcac ttgctattac gacgagcacc ataactcaca cagccgccta
      541 aactagattg atttttctgt tttaaaacaa tccgctgata tttccattgt cgtcgatgtc
      601 gatcagttcg gcagacgggt ttttcggtaa tccgggcatc ctcattattt ctccggcaat
      661 gggaataata aatccggctc ctgacgctat ttctatttcg cgtatattga cgataaaacc
      721 atgtggtcgg ttttttagtt ttggatcatc cgaaagcgaa ttttgcgttt ttgcaatgca
      781 aacagggagt ttatccaatt tgaggctcgt aatggttttt agttgagtac gggctttcaa
      841 ggaatattcc acaccggcag caccatacat ttttttagca atggtttcta ttttctgctc
      901 taccggccac gaaaaatcgt ataatggtcg gaaacataca cggcacgata gggcttctcc
      961 ggctacaatt tctgccaatt ccagcgcacc cttccctcct ttttcccatg catcgttcac
     1021 agcaacccga attccaattt cctcgcaatg tttgcgtatc atttgcagtt cttcagcact
     1081 gtcgcttgca aagcggttaa tagctacaat gggtttaaaa ttgaacatct gcatattctc
     1141 gatatgcttg tcgagatttg ccaacccaat attcaacgcg attgtatcgg gttgcgacaa
     1201 gtcagaaagc gatttcccac catgatattt aagcgcacga acagtggcaa caatgacaac
     1261 agcattggga ttcaaacccc catattgcga tttgatatcg aagaactttt cagcacccag
     1321 gtctgatgcg aaacctgctt ctgtaacaac aaaatcgctc agcgataaac ccatttttgt
     1381 ggcaataatc gaattggtgc cctgggctat attggcaaaa ggtccgccat gaataattgc
     1441 aggggtgttt tcaagagtct gcaccagatt gggctttatg gcatccttca acaaggctgc
     1501 cattgcgcct tccgctttga ggtcacgggc aaatattggc ttattatcgt aggtgtaacc
     1561 aataaatata ttccccagcc gttctttcag gtctttcaga ctttcggcca ggcataggat
     1621 tgccattacc tctgatgcag cagtgatatt aaatccggtt tcgcggggga ctccctgagc
     1681 ctttccgcca agtccgatta ctacatcgcg caacgagcga tcgttcatgt ccatcactct
     1741 tttccagctg atggttcg
//
LOCUS       DAOOQP010000220         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2568
            NODE_94526_length_1662_cov_4.613566, whole genome shotgun sequence.
ACCESSION   DAOOQP010000220 DAOOQP010000000
VERSION     DAOOQP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864643
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,596
            CDSs (total)                      :: 2,557
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94526_length_1662_cov_4.613566"
                     /isolate="AMR_MDS_2568"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLK75_12970"
     CDS             complement(<1..267)
                     /locus_tag="PLK75_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40203.1"
                     /translation="MLNFLIVIFAVSLIYISVAERFRAYAKLIAAQGVLLMGIALLEL
                     KEVNTANLIFIVAETLIFKTIVVPYLLFKIIGRTGIYKVHAKALP"
     gene            complement(270..1190)
                     /locus_tag="PLK75_12975"
     CDS             complement(270..1190)
                     /locus_tag="PLK75_12975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOY40204.1"
                     /translation="MPGFGFIMILMAAFFFPGIILRTKSLASGRKGPGVLQPVKDIMV
                     LLRKGSVFSETSGIIFRIAPAITLASIVCAMLVIPFGDDKALISFDGDFLFFAYLLAL
                     GKFFAIIAALDTGSSFEGMGANREALYSMLVEPAFFILLGTFAMFTGYTSFSDIFTHF
                     YITGNHYVLIYSLIGIYLFIQIAMIENSRLPVDDPKTHLELTMVHEVMILDNSGFDKA
                     LIHIGTYLKFAVYGALMCVVLVPAKWNLVLQIALFFAIQFVFAVVIGLIESFRARNKM
                     AKNPKFILTLSAIALIAFVVVLILTGKLIN"
     gene            complement(1192..>1662)
                     /locus_tag="PLK75_12980"
     CDS             complement(1192..>1662)
                     /locus_tag="PLK75_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY40205.1"
                     /translation="APSGFTTLHSVSQISIIGGIFVVLVTVLLTWRHFHMKNKTVVAG
                     PTWGCGYTAPSPKLQYTATSYADNYLQLANPLLRSKVTSDTIEGEEYFPGVKRFETHS
                     KDVVKTAMTDAPVNKFLELLKKIAVMQTGQIQHYILYAFVFMLLIFVLTYFNLI"
BASE COUNT          565 a          357 c          309 g          431 t
ORIGIN      
        1 cggaagcgct ttagcatgaa ctttataaat accggttctg ccgataattt taaaaagtag
       61 atatggaaca acaatcgttt taaatatcag cgtttcagca acaataaaaa taaggtttgc
      121 agtatttacc tctttcagct ccagcaaagc aattcccata agcaaaacac cttgggcggc
      181 aatcagtttg gcataagcac gaaaacgctc cgcaacactg atgtaaatca ggctaacagc
      241 aaatattacg attaaaaagt tcagcataat cagttaatca gtttacctgt taatatcagt
      301 accaccacaa aagcaatcaa tgcaattgct gacagggtga gaatgaattt cggattttta
      361 gccattttat tcctagccct gaacgattca attaaaccaa ttactacagc aaatacaaac
      421 tgaatggcaa aaaacagggc aatctgaagt acaagattcc actttgcagg cacaagaacc
      481 acacacatga gggcaccata gacagcaaat ttaaggtaag tgccaatatg aataagggct
      541 ttatcgaatc cgctgttatc gagaatcatc acttcgtgca ccatagtgag ctccaaatgg
      601 gttttggggt cgtcaacggg taagcggctg ttttcgatca tagcaatttg tataaataga
      661 taaataccta tcagactgta tatcaacaca taatgattac cggtaatata aaaatgtgtg
      721 aatatatcgg aaaatgaagt atagcctgta aacatggcaa aagtacccaa aaggataaaa
      781 aacgccggtt ccacaagcat cgaatacaag gcttcacggt tagcccccat cccttcgaag
      841 ctgcttcctg tgtcaagcgc tgcaattatt gcaaaaaact tacccaaagc tagcaggtac
      901 gcaaaaaaca aaaaatctcc atcaaacgaa atcagtgctt tgtcatcacc aaaaggtatc
      961 accagcattg cacaaacaat acttgccagt gtaatagccg gagctatcct gaaaataatg
     1021 cctgaagttt cactgaaaac acttcctttt cgcaacagaa ccatgatatc tttcacaggc
     1081 tggagtacac caggaccttt tcgacctgat gccagactct ttgtacggag aattattccc
     1141 ggaaagaaaa atgctgccat caatatcatg ataaatccaa atcccggcat gttagatcag
     1201 gttgaaataa gttaatacaa aaatgagtag cataaacaca aatgcataca aaatataatg
     1261 ctgtatctgc cctgtctgca tcactgcaat ttttttcaac aattcaagaa atttatttac
     1321 aggggcatcg gtcatggctg tttttaccac gtccttgcta tgggtttcga agcgttttac
     1381 acccggaaaa tattcctcac cctctatggt atcggatgta actttactgc gtagcaatgg
     1441 atttgccagt tgcaagtaat tgtcggcata ggaagttgcc gtatattgta gtttcggtga
     1501 aggagctgtg taaccacatc cccaggttgg acctgcaaca acagttttat tcttcatatg
     1561 gaaatgacgc catgtaagca acactgtaac cagcactaca aatattccac cgataatgct
     1621 gatctgactt acactgtgca acgtagtaaa accggagggg gc
//
